LOCUS       DARNVM010000001        83963 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.001, whole genome shotgun sequence.
ACCESSION   DARNVM010000001 DARNVM010000000
VERSION     DARNVM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.001"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..87
                     /locus_tag="SI621_000001"
     CDS             <1..87
                     /locus_tag="SI621_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HEI4062773.1"
                     /translation="DKNCSYDYLLHPAISISKIIGGCGPTAQ"
     gene            complement(194..1279)
                     /locus_tag="SI621_000002"
     CDS             complement(194..1279)
                     /locus_tag="SI621_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEI4062774.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTRGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLIPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSTLKQVVDEEQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1291..2595)
                     /locus_tag="SI621_000003"
     CDS             complement(1291..2595)
                     /locus_tag="SI621_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HEI4062775.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNALYWLGLFVPA"
     gene            complement(2607..2960)
                     /locus_tag="SI621_000004"
     CDS             complement(2607..2960)
                     /locus_tag="SI621_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HEI4062776.1"
                     /translation="MKKIGVAGLQREQIKKNIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMVAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(2971..3849)
                     /locus_tag="SI621_000005"
     CDS             complement(2971..3849)
                     /locus_tag="SI621_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEI4062777.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMIDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAFVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3846..5072)
                     /locus_tag="SI621_000006"
     CDS             complement(3846..5072)
                     /locus_tag="SI621_000006"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEI4062778.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSAIWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITVNLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFITHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5072..6235)
                     /locus_tag="SI621_000007"
     CDS             complement(5072..6235)
                     /locus_tag="SI621_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HEI4062779.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAALPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6319..6681)
                     /locus_tag="SI621_000008"
     CDS             complement(6319..6681)
                     /locus_tag="SI621_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEI4062780.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFTLEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6698..7603)
                     /locus_tag="SI621_000009"
     CDS             complement(6698..7603)
                     /locus_tag="SI621_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4062781.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7764..8768)
                     /gene="trpS"
                     /locus_tag="SI621_000010"
     CDS             complement(7764..8768)
                     /gene="trpS"
                     /locus_tag="SI621_000010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEI4062782.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8761..9519)
                     /gene="gph"
                     /locus_tag="SI621_000011"
     CDS             complement(8761..9519)
                     /gene="gph"
                     /locus_tag="SI621_000011"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEI4062783.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGITPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9512..10189)
                     /gene="rpe"
                     /locus_tag="SI621_000012"
     CDS             complement(9512..10189)
                     /gene="rpe"
                     /locus_tag="SI621_000012"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEI4062784.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10207..11043)
                     /gene="dam"
                     /locus_tag="SI621_000013"
     CDS             complement(10207..11043)
                     /gene="dam"
                     /locus_tag="SI621_000013"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HEI4062785.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11150..12436)
                     /gene="damX"
                     /locus_tag="SI621_000014"
     CDS             complement(11150..12436)
                     /gene="damX"
                     /locus_tag="SI621_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HEI4062786.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKVASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12528..13616)
                     /gene="aroB"
                     /locus_tag="SI621_000015"
     CDS             complement(12528..13616)
                     /gene="aroB"
                     /locus_tag="SI621_000015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEI4062787.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13673..14194)
                     /gene="aroK"
                     /locus_tag="SI621_000016"
     CDS             complement(13673..14194)
                     /gene="aroK"
                     /locus_tag="SI621_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEI4062788.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14596..15834)
                     /gene="hofQ"
                     /locus_tag="SI621_000017"
     CDS             complement(14596..15834)
                     /gene="hofQ"
                     /locus_tag="SI621_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HEI4062789.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15746..16150)
                     /gene="hofP"
                     /locus_tag="SI621_000018"
     CDS             complement(15746..16150)
                     /gene="hofP"
                     /locus_tag="SI621_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HEI4062790.1"
                     /translation="MRGKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGANCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16140..16580)
                     /gene="hofO"
                     /locus_tag="SI621_000019"
     CDS             complement(16140..16580)
                     /gene="hofO"
                     /locus_tag="SI621_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HEI4062791.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAFWLMMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16564..17103)
                     /gene="hofN"
                     /locus_tag="SI621_000020"
     CDS             complement(16564..17103)
                     /gene="hofN"
                     /locus_tag="SI621_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HEI4062792.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNSLETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(17103..17882)
                     /gene="hofM"
                     /locus_tag="SI621_000021"
     CDS             complement(17103..17882)
                     /gene="hofM"
                     /locus_tag="SI621_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HEI4062793.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18002..20554
                     /gene="mrcA"
                     /locus_tag="SI621_000022"
     CDS             18002..20554
                     /gene="mrcA"
                     /locus_tag="SI621_000022"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HEI4062794.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20722..21282)
                     /gene="nudE"
                     /locus_tag="SI621_000023"
     CDS             complement(20722..21282)
                     /gene="nudE"
                     /locus_tag="SI621_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEI4062795.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21602..23737
                     /gene="igaA"
                     /locus_tag="SI621_000024"
     CDS             21602..23737
                     /gene="igaA"
                     /locus_tag="SI621_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HEI4062796.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEEHSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23802..24470
                     /gene="yrfG"
                     /locus_tag="SI621_000025"
     CDS             23802..24470
                     /gene="yrfG"
                     /locus_tag="SI621_000025"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HEI4062797.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24481..24882
                     /gene="hslR"
                     /locus_tag="SI621_000026"
     CDS             24481..24882
                     /gene="hslR"
                     /locus_tag="SI621_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HEI4062798.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24907..25785
                     /gene="hslO"
                     /locus_tag="SI621_000027"
     CDS             24907..25785
                     /gene="hslO"
                     /locus_tag="SI621_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEI4062799.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25919..27643)
                     /locus_tag="SI621_000028"
     CDS             complement(25919..27643)
                     /locus_tag="SI621_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEI4062800.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYGYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKSSL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            28022..29644
                     /gene="pckA"
                     /locus_tag="SI621_000029"
     CDS             28022..29644
                     /gene="pckA"
                     /locus_tag="SI621_000029"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEI4062801.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKSLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29761..30078
                     /locus_tag="SI621_000030"
     CDS             29761..30078
                     /locus_tag="SI621_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4062802.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30137..30433
                     /locus_tag="SI621_000031"
     CDS             30137..30433
                     /locus_tag="SI621_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4062803.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEEAPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30465..31817)
                     /gene="envZ"
                     /locus_tag="SI621_000032"
     CDS             complement(30465..31817)
                     /gene="envZ"
                     /locus_tag="SI621_000032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HEI4062804.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(31814..32533)
                     /gene="ompR"
                     /locus_tag="SI621_000033"
     CDS             complement(31814..32533)
                     /gene="ompR"
                     /locus_tag="SI621_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEI4062805.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32761..33237
                     /gene="greB"
                     /locus_tag="SI621_000034"
     CDS             32761..33237
                     /gene="greB"
                     /locus_tag="SI621_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEI4062806.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33334..35655
                     /locus_tag="SI621_000035"
     CDS             33334..35655
                     /locus_tag="SI621_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEI4062807.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            36095..36322
                     /gene="feoA"
                     /locus_tag="SI621_000036"
     CDS             36095..36322
                     /gene="feoA"
                     /locus_tag="SI621_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HEI4062808.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36339..38660
                     /gene="feoB"
                     /locus_tag="SI621_000037"
     CDS             36339..38660
                     /gene="feoB"
                     /locus_tag="SI621_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HEI4062809.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVNIELLATRKSVSSCCAASTTGDCH"
     gene            38660..38896
                     /gene="feoC"
                     /locus_tag="SI621_000038"
     CDS             38660..38896
                     /gene="feoC"
                     /locus_tag="SI621_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HEI4062810.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQALNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKVCLREWWALR"
     gene            39099..39992
                     /gene="rpnA"
                     /locus_tag="SI621_000039"
     CDS             39099..39992
                     /gene="rpnA"
                     /locus_tag="SI621_000039"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HEI4062811.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(40212..40982)
                     /gene="bioH"
                     /locus_tag="SI621_000040"
     CDS             complement(40212..40982)
                     /gene="bioH"
                     /locus_tag="SI621_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEI4062812.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQSLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            41020..41703
                     /gene="gntX"
                     /locus_tag="SI621_000041"
     CDS             41020..41703
                     /gene="gntX"
                     /locus_tag="SI621_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HEI4062813.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARSDKTLCPQCGLPA
                     THAHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRLLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41762..42337
                     /gene="nfuA"
                     /locus_tag="SI621_000042"
     CDS             41762..42337
                     /gene="nfuA"
                     /locus_tag="SI621_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HEI4062814.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42698..44014
                     /gene="gntT"
                     /locus_tag="SI621_000043"
     CDS             42698..44014
                     /gene="gntT"
                     /locus_tag="SI621_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HEI4062815.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44062..46143)
                     /gene="malQ"
                     /locus_tag="SI621_000044"
     CDS             complement(44062..46143)
                     /gene="malQ"
                     /locus_tag="SI621_000044"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEI4062816.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLEDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFADCWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKK"
     gene            complement(46153..48546)
                     /gene="malP"
                     /locus_tag="SI621_000045"
     CDS             complement(46153..48546)
                     /gene="malP"
                     /locus_tag="SI621_000045"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HEI4062817.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVENTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49169..51874
                     /gene="malT"
                     /locus_tag="SI621_000046"
     CDS             49169..51874
                     /gene="malT"
                     /locus_tag="SI621_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HEI4062818.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALINRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(51917..52933)
                     /gene="rtcA"
                     /locus_tag="SI621_000047"
     CDS             complement(51917..52933)
                     /gene="rtcA"
                     /locus_tag="SI621_000047"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEI4062819.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVHGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFICRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVAAEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVASFSL
                     HEQNIHSLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(52937..54163)
                     /gene="rtcB"
                     /locus_tag="SI621_000048"
     CDS             complement(52937..54163)
                     /gene="rtcB"
                     /locus_tag="SI621_000048"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HEI4062820.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGTIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54350..55948
                     /gene="rtcR"
                     /locus_tag="SI621_000049"
     CDS             54350..55948
                     /gene="rtcR"
                     /locus_tag="SI621_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HEI4062821.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PENEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLLQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(55930..56688)
                     /locus_tag="SI621_000050"
     CDS             complement(55930..56688)
                     /locus_tag="SI621_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HEI4062822.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56705..57535)
                     /gene="glpG"
                     /locus_tag="SI621_000051"
     CDS             complement(56705..57535)
                     /gene="glpG"
                     /locus_tag="SI621_000051"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HEI4062823.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADSRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57580..57906)
                     /gene="glpE"
                     /locus_tag="SI621_000052"
     CDS             complement(57580..57906)
                     /gene="glpE"
                     /locus_tag="SI621_000052"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEI4062824.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            58096..59601
                     /gene="glpD"
                     /locus_tag="SI621_000053"
     CDS             58096..59601
                     /gene="glpD"
                     /locus_tag="SI621_000053"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEI4062825.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYSARLRRRYPFLTESLARHYARTYGSNSELLLCNAGAIGDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(59816..60416)
                     /locus_tag="SI621_000054"
                     /pseudo
     CDS             complement(59816..60416)
                     /locus_tag="SI621_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60419..61411)
                     /locus_tag="SI621_000055"
     CDS             complement(60419..61411)
                     /locus_tag="SI621_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEI4062826.1"
                     /translation="MNKFTCVLLLGTALLAGCVDREGYYNSVREEDSHGLTSLRGQPE
                     LRYNDDWSRWPRVYGATALYPLYASAYYELVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGTATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDIKNPVNSLSEQQ
                     VKDIFSGKVSRWNKVGGGDEGIKIWQRPEDSGSQTVMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIIHAYMVTHENPTLETQKFVDWFLSPQGQQLVEDVGYVPIYDASSESSGQ"
     gene            complement(61437..62945)
                     /locus_tag="SI621_000056"
     CDS             complement(61437..62945)
                     /locus_tag="SI621_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEI4062827.1"
                     /translation="MMQNGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEFC
                     NLLFTPTIYLLLNVFMLTLGALLLFFSGRVWAGDSAPENREIAAWRQCLFLVPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFTVSFVGGEYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFILLLLGIVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYVVDAFMVTRDNMTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTMK"
     gene            complement(63071..65518)
                     /gene="glgP"
                     /locus_tag="SI621_000057"
     CDS             complement(63071..65518)
                     /gene="glgP"
                     /locus_tag="SI621_000057"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HEI4062828.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(65537..66970)
                     /gene="glgA"
                     /locus_tag="SI621_000058"
     CDS             complement(65537..66970)
                     /gene="glgA"
                     /locus_tag="SI621_000058"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEI4062829.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(66970..68265)
                     /gene="glgC"
                     /locus_tag="SI621_000059"
     CDS             complement(66970..68265)
                     /gene="glgC"
                     /locus_tag="SI621_000059"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEI4062830.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(68283..70256)
                     /gene="glgX"
                     /locus_tag="SI621_000060"
     CDS             complement(68283..70256)
                     /gene="glgX"
                     /locus_tag="SI621_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEI4062831.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(70253..72439)
                     /gene="glgB"
                     /locus_tag="SI621_000061"
     CDS             complement(70253..72439)
                     /gene="glgB"
                     /locus_tag="SI621_000061"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEI4062832.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(72712..73815)
                     /gene="asd"
                     /locus_tag="SI621_000062"
     CDS             complement(72712..73815)
                     /gene="asd"
                     /locus_tag="SI621_000062"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEI4062833.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYSHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            74007..74600
                     /gene="yhgN"
                     /locus_tag="SI621_000063"
     CDS             74007..74600
                     /gene="yhgN"
                     /locus_tag="SI621_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HEI4062834.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(74646..75941)
                     /locus_tag="SI621_000064"
     CDS             complement(74646..75941)
                     /locus_tag="SI621_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEI4062835.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAIASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVLVKPNQDVQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISMWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(75848..77944)
                     /locus_tag="SI621_000065"
     CDS             complement(75848..77944)
                     /locus_tag="SI621_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEI4062836.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRLS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(78084..79424)
                     /gene="gntU"
                     /locus_tag="SI621_000066"
     CDS             complement(78084..79424)
                     /gene="gntU"
                     /locus_tag="SI621_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HEI4062837.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(79428..79955)
                     /gene="gntK"
                     /locus_tag="SI621_000067"
     CDS             complement(79428..79955)
                     /gene="gntK"
                     /locus_tag="SI621_000067"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEI4062838.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(80094..81089)
                     /gene="gntR"
                     /locus_tag="SI621_000068"
     CDS             complement(80094..81089)
                     /gene="gntR"
                     /locus_tag="SI621_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HEI4062839.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(81313..82008)
                     /gene="yhhW"
                     /locus_tag="SI621_000069"
     CDS             complement(81313..82008)
                     /gene="yhhW"
                     /locus_tag="SI621_000069"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HEI4062840.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVYQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(82131..83168)
                     /locus_tag="SI621_000070"
     CDS             complement(82131..83168)
                     /locus_tag="SI621_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEI4062841.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            83503..>83963
                     /gene="yhhY"
                     /locus_tag="SI621_000071"
     CDS             83503..>83963
                     /gene="yhhY"
                     /locus_tag="SI621_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4062842.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFIDNAPAIKVYKKFGFEIEGTGKKYALRNGEYV"
BASE COUNT        20419 a        22892 c        20886 g        19766 t
ORIGIN      
        1 gataaaaact gctcttacga ctacctgctg cacccggcaa tttctatttc taaaatcatt
       61 ggcggttgcg gtcctactgc acagtaagtc tctgactgaa agcggctaac accggtttaa
      121 ttgcctgacg ccctcggggt tacccgaggg cgtatttatt tctgcattct tttcgaaaac
      181 atcatgcaca agattaaata ctggcaatac tctcacgcaa aatccgcagc accgtctctt
      241 caccgctgcg atttgggtta atacgaatcg cgtaatgttc gagttgcgga ttcgcctggc
      301 gaaacgttcc ggaaaggcga taaaagagcg gcgggatttc atatttcgac tctgcaccga
      361 ccgggtaagg caacgcgccg cgcttttgcg cctcttccag tactctggcg gcaatcggct
      421 gatgaaactc cacaatcaac accttcgact gcgcattggc aatcaccgcg cttttcactt
      481 ccgccactgc gccaccgtta agtaaagcca gcaaccgctc agataccccc gcctgcaccg
      541 cgtgcatcac tggcgcaaac accagcccac gcaagacttc cagcgcctga gcgccctgaa
      601 tctggctgcc gccggaatag agcgtggcgc gaatacggct aataacatcg gcatcgccta
      661 ccaccgcgcc aacaccctct ggcccaaaca gcttgaagca ggaaaaagtc gagacattcg
      721 ccccgcattc gcagccgatt cgcgctacct tcatcaccgc atagttgtca tcggttaacg
      781 ctggaacacc tgccgcgcgc aacgttgcca gcacgtctgc cagcacatag cggtcctgcg
      841 gctgctggcg cgtatgctgc accagcgctg catcaggttg ttcctcgtca acaacctgct
      901 tcagtgtcga cagatcattg aaatcagccg taatgagcgt tagccccatc tgctcaataa
      961 taacccgtgt cgtcgggtaa acaggcgcgt catgcaccag aagacgctgc cccggtatga
     1021 gcaaagctgc cagcgccgca cggatcgcgc cagtacccgc gccctgtacc agcgccgcgg
     1081 cctgtgcgtg aaacgcgtca gccagcacct gttccactcg ctgggtaatg cgcggttgat
     1141 tcagccccgg cgttaagccc aaatccccgc gggtaagaaa ctcgctgccg ggaaaatggc
     1201 ggcaaatcgt gtccaccagc gcaaactgct tttgctgcgc ctcaataagc gtcaggcttt
     1261 gcagaggaaa cgtcttcata ggtgctttcc ttaagccgga acgaacaggc caagccagta
     1321 aagagcattt agcagaatac cagttatcat tactgccacg actggtgccg ccattttctg
     1381 taccggacgg cccagggatt cgttgaggaa gtaaatggca actgcgatag agaatccggt
     1441 ataacccgcc attttaattg ctgcgaaaat cgaaccgacc agcaacgcca cttccatcag
     1501 catattcatg gcattacgga tgttatccga cgcattacgc accgacgggt agcgccccag
     1561 ccatttgccg atcgaacgaa gcagcaagac ttccgccgaa ataaccactg cgcctaatac
     1621 cgctgcaacc atcggattcg gcgagagata gcccaccgca taaacaaagg taaagcccgc
     1681 aactgcatac acaccagtgg ctaacgcggt ggtggcaatc aacggtacaa aacccagccc
     1741 gcgcataaat tccgccagag ctgcctggtt aatcagcgtt tgtgattgtt ccggcgttac
     1801 gcctgcggaa tatgctttct ccagtgtgaa gatcgacact tcactgccag caaaaatctt
     1861 catgctggca acggcggcaa tcaatgctcc cacgatggcg atatacggta agtttttgat
     1921 aatccgtgac gtgcgttctt caaacaccga aagcccgctg gcatcaatgt cattttcatc
     1981 acgatgacgc aggtcgtgag ttatcgcgat ccccagcagc atcaccatac caataaagat
     2041 ttcgatggat tcagggttaa gatgtggaaa atagcgcacg acgaccacac gggtcatcag
     2101 taccaccacg gcggcgatca gactttgctt ccagccaaac tggtagaaaa tcgccaccag
     2161 cgggaagagt gcaaaagctg aaaccaccgg cgagcttaat tcccccagac tacctaatac
     2221 atccaccggt agcgcggtca gcagctggtt tactggcaac agacaggtga ggatcaacac
     2281 gccccagata gcgccaagac caaacgccat caggctgttt atcgctagta cgccgaggat
     2341 atcggtagga agaaagagta accatgcgtt gagcagcccg gttttcaggg taaacgagat
     2401 ccccaccgag gccacaaaac caatgctcag accaaaagcg atactccccg cctcgcgacg
     2461 gttcatatag ccttcaatca gttgcggcag gatcgggcgg ataccgtcat gaaaaacggc
     2521 cgccgagcga tgcgccagaa gcgatgtcat acccgtcagg cacgccacca cgataatctg
     2581 aatatacaga tccatagtcg tgccttttat ttcagatggt taatcagcat cggaatcgcg
     2641 tgttcgacgt gctcaacgga aaggccaaac gccacttttc cttcagcgac cattttggcg
     2701 atatgctcgt ctttcgcttt aatgcccggc ttggcaatgg tgcaactttt gttatagcca
     2761 atcaccgcga tggcaatcga caatgccgcg cccgcaccgg tattacacgc gccgatgtag
     2821 taatccagtt gaccggattt cactttcatt gccgcttcca tgtcgttgtg aatgaaaact
     2881 tcaaaacagc caggagccgt cgcttcaatg ttttttttaa tctgctcacg ctgtaagcct
     2941 gcaacaccaa tctttttcat gagtctgtcc ttattggaaa aattgagagg gagtttcacg
     3001 taacatcaca tccacatcgg cctgactgaa tcctgactgg cgcaattgcg gaataaaggt
     3061 ggtcaataaa tagtcatagc cataaccacc gttggctttt aaatgggagc ggcgcgtaat
     3121 atccatcgac agcatgacgc ggttcagcaa accacggtca cgtagtgcgt ggagcatcgc
     3181 aatgcgcttt tcgtccgggt agtaactgtt cttgccgatg gtgtcgaact gcacaaacgc
     3241 gccgagatcg atcatcttca aaatgttgtc gaggttgtct ttcagatcgc agtgaccaac
     3301 ggtgacgcgc gaaagatcaa ctccgtgggc ttgcagtagc gccagttgtt ccagccccat
     3361 ggtgctaaaa gatgtatgcg tggagatcgg acgtccggtc tggttatgtg ccagcgcggc
     3421 ggcaataaac accttctctt ccagcggcgt aatcttccct tcgctggagc cgatctccgc
     3481 gatgatcccg gctttcagct ccgtgccatc gataccctgt tcaatttcat cgatcatctc
     3541 ctgcgccagt tcctgcacgc tgcgggtcgc cacatgttcc gggaaaaacg cgtcctggta
     3601 ataaccggta caggccacca cgttgatccc ggtttcgcgc attacatcga gcataaattg
     3661 cgcattgcgc cccatgtaac ggttagtcat ctcaatcaca ttacgcacgc cccgggccat
     3721 caggtcgttc atctcctggc aaatgaacgc atactgatca aggcggcagt ccacgttgtt
     3781 tttaaagccg gagagatcaa tatgcagatg ctcatgggcc aaggtgtaac ccgtcggatc
     3841 aaaactcata gggtgtctcc tgcaaaccgg agggaggaaa gaaaagagcg accattttgt
     3901 ggcggtggcg cacggaaaaa ttcacacacg gtagccccca catcagaaag cgtggtgcgc
     3961 acaccaagct gcgtagcgac cagcccttgc tgataaacca gcactggcac cacttcgcgg
     4021 gtatggtggc tgtgaccaat ggtcggatcg ttaccgtgat ccgccatcac gaccaggcaa
     4081 tcatctggct gcatcgcctc aataagccgg gcaaggttac ggtcaacgac ctgcaaacgt
     4141 tcggcataac gtgcgacgtc ctccgcgtga ccagcgaggt cggtttcctg aatgttggtg
     4201 caaataaacg ctgtctgatg ggtgataaat tcgtcgaggg tgatatccat aatccgctgg
     4261 ctatccacca ggttttgcca gctcacgcca taaggattgt tgacgatatc cgccacctta
     4321 cccaccagca cggttggcac gcccgcttca tacagttttt gcggcacctg cactttttca
     4381 tcaacaccgt agcccatatg cacgacctgg aagccgttgt cataagcgcc tgaacgcggc
     4441 gcattaatgc caataaagcg cccttcttta ctttctgcgg catcgagaat gcgttgactg
     4501 tcagttaaca ggccgccaaa tgtaatgacc cgaccgacct gtacctgctc acgcacgata
     4561 cgaccaattt tgattgcgtc gtcaaaagag atcacagaga gattgacggt gatgttatag
     4621 acctggccta aatccgcctc gagattatcg ccaatcgcaa ccgcctgatt gacccacaga
     4681 aattgcagat catcgccacg gcgctccacc tgccaaccgg cggatactaa tgcctgctca
     4741 acacggtcaa tcacatcgcg aaagggcatc ctcagcggcg gtaacggacg cgtgcctaaa
     4801 atttcctgat gccccataaa ggtatcgcca ccttcatgtt gcagctctgc cacaccccag
     4861 attgcggagt ctgacggctg catatcacct ggcgcataac ccaatgcgtt gattagcccc
     4921 agcttctcca gcgttggtag ctgcaaatgc ggcagctggc tcaggatgtg accacaagta
     4981 ttcgccccgg catcctgtgg acgcaccagc gtgacatctt tcattgcgcc tacgccaaag
     5041 ctatcaatca ctaacaccac aaatcgcgcc attacgcccc cagagagttt ccaagactgt
     5101 cataacgacc gacgatttct ggttcgccac ggtgaatacc ggacagcaac accacatcgc
     5161 tacgggtgac aaaaatctgc gtgcgaaaac agagcactac tgcactgctt accggaaact
     5221 cgcctgccag cggcagggtg tagtcgatac tgctgtcatc cacagttttg agattggttt
     5281 cagtaatctt ttgattttct ggcgtaaaca ccagcgcatg ttgcgcatga ccacgacggt
     5341 agtaaccgcc gccgtagcag tagctgtcgc cacgaaaatg atgggagatt tcacttaacc
     5401 agagcatcgc gatacgttca ggctgatcgc cctgctggtt tgccggaata gtgcccgtca
     5461 gcgcatgacc aggttcggca tgagtcacac cgtattccgc cagcaatggc agcgaagtgc
     5521 agctggtcgc agaaggtgcg ttcagttgct caatcgcaat accagatttc gccagttgat
     5581 cccgtgcctg tatcagcgtg tgaagattcg gtgtcggcaa aactttcccg gcagcctcat
     5641 cccaaagcag gcaagggaaa tgggttagtc cggctaaatg caaccccggc agtttcttaa
     5701 tttcagcaac cacctcgtgc agcgaatgct gtacaaaacc gctctcctgt cccggataaa
     5761 gaaaatcatc gtcgctataa actttgagca gcacagactg cacacgtccg gccttcaccg
     5821 ctgccgcaga aacttcccgc gctttgtcga gagtaaacac ggtgatgacg tcggtgccct
     5881 gtgcaacggc gtcaaccacc tgatgacaag ggatttgtac taaatgcccc tgatgcgcca
     5941 ccggcaaagc agcgcggcgc atgactcgcg cctctttgta atccaccgcc acaatgccgc
     6001 tgtagcccaa tgccagcagt ttttccgcca gccatggatt acgaccaaac tgcttagtca
     6061 tcagatacag ttcaatgccg taaagccgcg ccgtctcaat cagccgctta ccgttttcca
     6121 gtacctgatc cacgtcgatc acccagctgt ccggggcgat cttgccctgc tgccacaaac
     6181 ttagtgcagc ggaaatcagc gccggattct ggcgtttcaa tgcttctaca aacatcttgt
     6241 tgcctcgttt ctgttccttg aattaaatat tcattttttt gaatatttag gttgtgcgac
     6301 gtggatttgt cgggagcttc aaacctcttc gttagcagcc atccataatc cataaaggtt
     6361 ggccagcaaa tagccttctt cacacgggtt aacttccagt gtaaattcct tcaacaacac
     6421 ctgatgcaat tgcacaacgg cttgccagtg gctggattgc gccagttctg ccagcaattc
     6481 ggcgtccagc ggctctattt cttcaccacg gcgactgcgc atcagtgcgc tcgccatatg
     6541 tgtcatcgcc atcgttcctt gctcactacg caccggcaga tggcactctg tttccagtac
     6601 gttgacgacc tgcatcatgc ctttgcagat gtccttatca ataacgcctg catcgcaaag
     6661 caggttgagt ctggtttcca tatcagatgc ctttcgttta ataactcggt tcctgttccc
     6721 cactcaccac tcgttgttga tgggcgagca gggcgtgttt atcaacaact gcacgcagca
     6781 gttgttgcat cgggtaatca tcggcacgcg tcaggaccac ggcttcggtg gcctgtaaaa
     6841 atcgcggatc gtctgtgagc ggcgtcgctt ctaatcccag catggtcagt tcgttttccg
     6901 ccaccacgtt ccagataacc gcatcgacat cgcctttaac aatgcgttgt aaactctcgt
     6961 gataagagag atcgacacgt tccacatcac tatcgccaaa aaagacatcg gtcatgattt
     7021 tctgatccgc cgaacggtta tccagcccca cgcgcttcac gtttgcggac tcgcctttac
     7081 ggcaaatcaa ctggtgctcg ccaacgtagg tgtgcggccc cagctccagc gcgaggcata
     7141 accctttttg cgtgagataa ctttccgccg ccagtcggga aacaaccgcc atgtcataca
     7201 cgccgttaag taaacactcc acgcgaatat ccgcgccgcg catgtgcgca tagtaaaaag
     7261 gaataccatc aaactgagct ttcaatccgc tcgccaggcc ttcgtacaaa cgggtatagg
     7321 gcaagggcat cgcgcacacc acattgttga tatccacatg actcagcagc gctttgttat
     7381 ccatttccac cagataactg ccattacgcc cacggcgttc tatccgtatc gctccgcttg
     7441 attccagcgt tttcaacgcg gcctgcgtca ggccaacgga tgaacggcat tcagttgcca
     7501 gttcatctat ggttttcagt cgattaccgc acttttcacc caacaaataa cgggccagtg
     7561 tcgtgatgac gacgccttct tttttgataa acgttcgacg catagtaaat tttcaataaa
     7621 gtgaatattt atatcttcat aaaaatgaag ataagtggca actgcgagat gtggaaacgg
     7681 cgaggcgctt cacatttttt cggattattg caaaaagagg ataaaaaaac cgggtttccc
     7741 cggtttttgg cattaatgtt aaattacggc ttcgccacaa aaccaatcgc ttcgtacacc
     7801 gcttttagcg tacgggaagc gtgcgcgctg gctttttctg cgccatcttt catcacctgt
     7861 tgcaggaagg cttcatcgtt gcggaaacgg tgataacgtt cctgcaattc agtcagcata
     7921 ccggaaacgg catcagccac ttcgcctttc agatgaccat acatcttgcc ttcgaactgt
     7981 ttttccagtt ccgggatgct ctggcccgtt accgcagaaa ggatatccag caggttggaa
     8041 acgcccgctt tgttctgcac gtcgtaacga actaccggcg gctcgtcgga gtcggtgacc
     8101 gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccgat aacgttattg
     8161 cggttatcgt cagacttgga catctttttg gtcggctcca gcagcgacat tacgcgcgcg
     8221 ccagatttcg gaataaacgg ctccggcact ttaaagatct cgccatacag cgcgttgaaa
     8281 cgctgggcga tatcgcggct cagttccagg tgctgtttct ggtcttcacc caccggtacc
     8341 agattagttt gatacagcag gatgtccgca gccatcagca ccggatagtc aaacagacca
     8401 gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat gcggctcagt
     8461 tcgccgaagt aggtatagca gttcagtgcc cagcctaact gtgcatgttc cggcacgtgg
     8521 gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata caaggccagc
     8581 gtatccagcg tcgctttacg cagcttctgt gcatcctggc gcacggtgat cgcgtgttgg
     8641 tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg acgcagcgca
     8701 cccatgtagt taccaatggt caattcacct gagggctgtg cgccactaaa aacgatgggc
     8761 ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg gcagaaggtc
     8821 atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc cgtagttata
     8881 tccgtaggtt aagccaactg atgggcaacc tgccgctttt gctgcctgaa tatcattgcg
     8941 tgagtcgcca acaaacagca tctgttgtgg ggtaattccc atccgctcag ccaccagtaa
     9001 cagcgggtcc ggatgcggtt ttttgttttg cacatcatca ccaccaatca ccacgctgaa
     9061 gtatttagcg atatctaagg cttccagcag cggcgcgacg aacggcgtcg gtttgttggt
     9121 gaccaggccc aacggcaggc ctttagcctg caatgcgccc agcgtatcgg caacgtgcgg
     9181 gaacaaaaac gtcccctctt cggcaacctc gccatagtag cgatcgaata gtttacgcag
     9241 aatacgtact tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac ccattgtttt
     9301 acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca gaacatctgc
     9361 gccgttacca atccaggtta taacgcgttc ttcacctgcg acgggcaact ccagcgcata
     9421 cagcgccata tctactgcgg tagccaagcc aggagcactg tccaccagcg taccatcgag
     9481 atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg acttaccttt gccagttcac
     9541 tgcgcatttc atcaatgact tttttgtagt ctggctggtc gaagattgcc gaaccggcga
     9601 cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt gttcaccttc acgccaccgt
     9661 ccacttccag tcgaatgtca aagccagact cgtcgatacg acgacgtact tcgcgcagtt
     9721 tatccagtgt ttgcggaatg aaagactgac cgccgaaacc agggttgacg gacatcagca
     9781 ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc gccgggttaa
     9841 ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg tcaacatgct
     9901 cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa tcaggcacaa
     9961 tgcgatcgac cggtttcacc atcaggtgta cgtcgatagg ggcggtaatg ccgtagttac
    10021 gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacatagtgg ttatccatga
    10081 cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct tcacccaggc
    10141 gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc cgcttctcct
    10201 tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca gcagttcgtc
    10261 caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga cgacatgcaa
    10321 ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga tcagcactgg
    10381 aatatggcga tcaaccagac cttcagcgat ctccgccaga tgcgcttgtt gttcaagcgt
    10441 aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggcg cataaggcgg
    10501 atcgcaatag acgacggatg catcatctgc gcgcgccatg ctatcggcgt aagactcaca
    10561 gtagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt ccgggaaata
    10621 gggttttttg tagcggccaa acggcacgtt aaactcaccg cgcagattgt aacgacacag
    10681 gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga acggatcctg
    10741 gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg tttcgggaac
    10801 aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa tgttatagag
    10861 actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg tgttgagaaa
    10921 caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca aatgccgttt
    10981 aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc gatttttctt
    11041 catgctgact aactaattac accttctccg gctgtggaga aagctccgac agcatccagc
    11101 gcttcaggca actcccgcaa cgataaacca ttgcgggaga taaccttgat tacttcagat
    11161 cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctggaca tcggctggca
    11221 atgtagatac cgcttttttc gcctcttctt tcgaggcgta cacgccagaa accaggacat
    11281 accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga ttctctttct
    11341 tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga gtgtaatggc
    11401 tggacggtgc cgatttcaac gaaccaacat tacctgcggt cttccctcca gcggctggtg
    11461 ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagttgcg gtctcttttg
    11521 gtgctggcgt agaggtcgca gccggtgcct tcgtgctcgc cacaggagca gctggttcag
    11581 tacgcttcgg cgtctgtgct accggcttcg gctccgtttt cacggttgct tgcggttttt
    11641 tcggttcaat caccgcctgc tgacgagctg ggcgcgtagt ggccggacgt tcagcggttt
    11701 gcgttttcgc cgtgtcacgc gatgcattgc cattgcgaac aggcgcgacc gtcgcgggtt
    11761 cagtcggcaa cgtggaattg accgccacat tattcaactg ttgctgattt tgtggctggg
    11821 tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc gttgccaccg
    11881 gggtttgccc ttgagtcggc gtagaagaga tcggcggcag agaaacatcc tgctgagtat
    11941 tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg gtcgcattgc
    12001 cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagag gtcgaggggg
    12061 cttttagcgc agaaccgata ccgatgatca acagcaggag aaccagaatg ccgacgccca
    12121 tcatcatata ctgacgagaa gcgggtttac tggctacttt tttgcgctta cgcggacgac
    12181 gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct tcttcgagtt
    12241 ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca gtgtcatcaa
    12301 gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca gaacgttcag
    12361 aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc tcgtcttctg
    12421 gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg ttgcaattaa
    12481 cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta cgctgattga
    12541 caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac ttcactctta
    12601 ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt gtcacgcagc
    12661 atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg taacccagcc
    12721 cgcgtgagca gggttataat acgctgcgtt tcggcagaac taaactgccc gagacgttcc
    12781 gacgtccgcg ccgccatcac catacccgca gcaaccgctt caccgtgtaa ccaattgcca
    12841 taccccattt cagcttcaat ggcatgacca aaggtatgtc ccagattcag taaagcacgt
    12901 aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca acaacggcga
    12961 atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt ctcttccagc
    13021 cagttgaaaa acgcaccgtc aagaataatg ccgtatttga tgacttctgc cagccccgac
    13081 gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac tgaagcaggc
    13141 tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt tttgccgcca
    13201 acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg tacaccgcgc
    13261 tgataactcg ccgccgcaaa gccggtcaga tcgcccacta caccgccgcc aagcgccacc
    13321 agcgtagtat cgcgaccatg cggtttttgt aacaacgccg taaagacggt atcgagtaca
    13381 gccaggcttt tatactgctc gccgtcaggg aggataacgc tatcgacgtt aacacccgcc
    13441 tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc gttggtaacc
    13501 aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt aaacaaacca
    13561 gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct ctccataacg
    13621 cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag aattagttgc
    13681 tttccagcat gtgaataatc tggtttgcga ccactttagc gctttgatca tcagtacgaa
    13741 tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac gcttccagaa
    13801 cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc tgcgtgcgtg
    13861 caagttgctt ttcgatggtc gtttcaagat aaacgacaac gccacgtgcg gaaagacggt
    13921 tacgcgtttc gcgggatttc acagagccgc cgccagtagc cagcacaata ccctgtttct
    13981 cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg ccttctaaat
    14041 cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg gaatcgtaaa
    14101 attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg gcacccatag
    14161 gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta agactattcg
    14221 ttaatgataa acccgcttcg ctcagagagc gccgcaggac atgaactgaa acctcataag
    14281 atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg caactgaata
    14341 aatcacccat ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct accaccgctc
    14401 tggcgataaa tcaccgggta agattagcgt aaaaaaagac agcaaaatgc cgcctgaatg
    14461 ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat cggtactcct
    14521 tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcgta aaaacattac
    14581 cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc
    14641 gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc
    14701 aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc
    14761 caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat caatcgccaa
    14821 cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt
    14881 cagccggatg cgaccttttt gtaacaccgt gggcgtaact tccatcccca ggacggcctc
    14941 tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct
    15001 cccctgttta atgctggcag gctgaagatg agaggccagc agacgcggac tggcgataat
    15061 atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagtaaac gtccgttgat
    15121 gcgtccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt cgctgccaag
    15181 cgtggtgact tgcccaacgc caccagcttg ttgcgcatca gccaacgtcc atttcacgcc
    15241 taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc
    15301 gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg ttttgttatc
    15361 tcgcagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag
    15421 cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagcgtta tactgcgatt
    15481 ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctgacggg cgatattgtc
    15541 attctgccag gcaacggaat gcaccgagag aatgttgccc tcctgacgcg ttatcagtcc
    15601 ggcgcttttc actacagttt gtagtgcttg cttccaggga acatctgtta gatgtaacga
    15661 caccgtaccg ctgacgtctg gcgacaccac caggttcaac ttctcctgtt cagccagcgc
    15721 ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc
    15781 cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat
    15841 ctccttgccg ttgccacaac cagtgtggcg gttcgcagtt tgccccggtt cccagcgtta
    15901 gtgcgtctgg cgtcagctgt aagatcgtcc agccgttttc cagcacatcg ttttgctgca
    15961 cccgtcgcca ttttttttgc ccatctttta ttacaccgat gatgcgctcg cctcgcccta
    16021 ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct tccggcggtt
    16081 taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac aaccagcgtt
    16141 tacccctcat gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc ttccacgctt
    16201 aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc cgacggcact
    16261 gcttcccaca gcgttttcaa cgccaactca cctccctgcg cggatggatg ccaggaaacc
    16321 agttgcgcgc cggataactg aaaatccagt ggcgaaaagg gcagcgtttt ttcttcgctg
    16381 aagggagtgg cgtctaccag gcgatacagt gcagcccact gctggtgatg actcgcccgt
    16441 agtcgaatta atgcgtcgct ctcttcctgg tgtgtcgatg acagaaaaat gagcgtaact
    16501 aacatcatca accagaatgc ccagcaaagc tggcggaggc ggggtgatgt ggcgaaccac
    16561 cagtcaaaga gcatgtccat cgctaacctt ccttgttaac tgatactcaa attgccagcg
    16621 tccctgcgca tcctgctgcg tggccccccg ctgattgaga tgaaaagaag catcctggcg
    16681 gagtgacgtt tccagcgagt ttaacgcggt aatgcttgtt gtcagcccct tgatctccag
    16741 cgttccctgc tgccagctta tcgttgtcag ccaggcatgt tcgggtaaaa gcgccgccag
    16801 tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc tttgcaaacg
    16861 ctgttcgcgt aactgctggc gttccagcaa acgtggtttc gttatctgta agctgcgggc
    16921 gagttgttgt tccgcctgaa gcaaaacggt gtctacgcgt gcttcggcgt tgcttgtcag
    16981 acgcagtatt agcgttatcc cgacagccag cagcagaggc gcaacgaaca tcagcaacca
    17041 gaaacgaaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa ttggcgggtt
    17101 catcagtaca ccttcccaag cgccagcccc agcgcgatgg caaagtctcc acctggcggc
    17161 ggtagcggcg gctgacgaac agaaaccgcc tcccaggggt caaatccgcc ttcgccacat
    17221 atcgcgacgc tttcgggatc aacggataac gctgccgcca gctccttcgc gctagttatc
    17281 cccaccgcca gtttgcgccc ccagctatag cgcgtcgccc acagccactg ttcgttatca
    17341 cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaatcgctg taatgcactg
    17401 gcatccgggg tgatcgcgct aacatgaaca cgcaaccttt ctgccagagt aagcagcgtt
    17461 gccaactctt tgctttgcgc ggcagtcacg ttataggcgg gactgagtga gtcttcgctg
    17521 taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt cccggacagc
    17581 caggccgttt gctcccgctc accaagggac atcgacgggc gtggaaatga ccgctgtaat
    17641 gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc gcgactccac
    17701 ggtaacaacg tgttagccag ccgctgcgca tcaacaatcc gcccatcttt gataatgtcg
    17761 tgcgccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgcgcc ccgcacgata
    17821 gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat cttaaatgcc
    17881 attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg tctggcttgc
    17941 ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg ggaaatttcc
    18001 agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc tgggagcagg
    18061 ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg cgacattaaa
    18121 agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc tgatcgctca
    18181 atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccgccgg agatggtgaa
    18241 agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg acccggtggg
    18301 gatcttccgt gcggcaagcg tggcgctgtt ctccggccac gcgtcacaag gggcgagtac
    18361 cattacccag cagctggcga gaaacttctt cctcagtcca gaacgcacgc tgatgcgtaa
    18421 gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag acgagatcct
    18481 cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg gtgctgcggc
    18541 acaagtctat ttcggtaaaa cggtcgacca actgacgctg aacgaaatgg cggtgatagc
    18601 cgggctgccg aaagcgcctt ccacctttaa tccgctctac tcaatggatc gtgccgtcgc
    18661 gcggcgtaac gtcgtgctat cgcggatgct ggatgaaggg tatatcaccc aacaacagtt
    18721 cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga ttgctttctc
    18781 tgcaccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg gcgaaagtgc
    18841 ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtacagc aggccgcaca
    18901 gcaggcagta cgtaataacg tgctggacta cgacatgcgc cacggctatc gcggcccggc
    18961 aaatgtgctg tggaaagtgg gcgaatcggc gtgggataac aacaagatta ccgatacgct
    19021 gaaggcgctg ccgacctatg gtccgctgct gcctgccgca gtcaccagcg ccaatcctca
    19081 ggaagcgacg gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg aaggcgttcg
    19141 ctgggcgcgt ccttaccgtt cggatactca gcagggaccg acgccgcgta aagtgaccga
    19201 tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat ggtggctggc
    19261 acaagtgccg gaagtgaact cggcactggt gtcgatcaat ccgcaaaacg gtgccgttat
    19321 ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca cccaggcact
    19381 gcgtcaggta ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg ataaaggtct
    19441 gacgctggca agtatgttga acgatgtgcc aatttcccgc tgggatgcag gtgccggttc
    19501 tgactggcag ccgaagaact caccaccgca gtatgccggt ccaattcgct tacgtcaggg
    19561 actgggccag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg gcgtcgacta
    19621 cgctgccgaa tatctgcaac gcttcggctt cccggcgcaa aacattgtcc acaccgaatc
    19681 gctggcgctg ggttcagcgt cattcacccc aatgcaggtg gcgcgcggct acgcggtcat
    19741 ggcgaacggc ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa acgatcaggg
    19801 cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata ttccggtaat
    19861 ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag atgtcgctat
    19921 ctcccgcgag cagcagaatg tttctgtgcc aatgccgcag ctggagcagg caaatcaggc
    19981 gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca ctccgctggc
    20041 attcctgatt aagagtgctt tgaacaccaa tatctttggt gagccaggct ggcagggtac
    20101 cggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa ccggtaccac
    20161 taacagttcg aaagatgcgt ggttttcggg ttacggtccg ggcgttgtga cctcggtctg
    20221 gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg gagcgattaa
    20281 agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat gggacgctta
    20341 tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgccgc cgggtattgt
    20401 gacggtgaat atcgatcgca gcaccgggca gttagctaat ggtggcaaca gccgcgaaga
    20461 gtatttcatc gaaggtacgc agccgacaca acaggcggtg cacgaggtgg gaacgaccat
    20521 tatcgataat ggcgaggcac aggaattgtt ctgattaaaa aggcgcttca gcgccttttt
    20581 aagtttgctg acaaagtgca ctttgtttat accggatacg gcgtgaacac tttacccgtc
    20641 tagcaacatc tagaaaaatc aatatattgc aggagccgca caggcctgat aagcattatg
    20701 catcaggcaa acttcacgca tttacaaccg cccctgcccc ttcaaccatt cgcgcacgag
    20761 gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca aatccatcat
    20821 atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt ccagcgattc
    20881 cgggtagaga tcttgcgcta ccacgatatt cattttgctg gaaaagtaag acggtgccat
    20941 gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat cccacctctt cttttagctc
    21001 gcggttcgcg gcttcgaaga cgctttcacc cggatcaatt aatcctttcg aaaaacctaa
    21061 ttcgtaggat tcagttccca ctgcgtattc gcggatcagg atcaggtgat cgtccacaat
    21121 cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa cacgccgcac
    21181 gccattgctg aactccagat ccacgctttc gacggtaaac agtcgggaac gggctacagt
    21241 ttcaacattc agaatggtgg gtttttgtaa tgatttgctc atcgtgggat ctatgctgtg
    21301 aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt gcatgttatt
    21361 tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc aataggttga
    21421 aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga tcagcggtta
    21481 ctgtgatgtg cttaatgatg cttaagttaa actccacgct tgccgatagc caaccgcaga
    21541 atcatgtatt gtgtccggtg cgactgacca cgcctgacag actaagtaag atggggaaag
    21601 catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac ttgcgggatg
    21661 gctgataaaa gtgcgatcca gacggcgtca gctgccctgg accaacgcct tcgcggatgc
    21721 gcaaacgcgt aaactcacac ctgaagaaca tagcgccgtt gaaaattatc ttgagagcct
    21781 gacgcaggta ttacaggtgc ctggcccaac gggagccagc gcggcaccga tctctctggc
    21841 gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacgc gttacggcat
    21901 ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag tccacctgcc
    21961 ccccttctgg gaacagtaca tcaacgatga gaataccgtt gaactgattc ataccgattc
    22021 gctgccgctg gttatttcac tcaacggtca tacgctgcag gagtacatgc aggaaactcg
    22081 cggctatgcc ttgcaacctg ttccgtcaac acaggcgtcg attcgcgggg aagagagtga
    22141 gcaaatcgag ctactcaata ttcgcaaaga aacgcacgaa gagtatgcgc taagtcgtcc
    22201 gcgcgggctg cgtgaagcgt tgctgattgt cgcctcattc ctgatgttct ttttctgcct
    22261 gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcgttactgc tgctgggcgc
    22321 aggtctgtgg gggctattcg cgcccccggc aaaatcctcc ctgcgggaaa ttcattgtct
    22381 gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac agatcaacaa
    22441 tatttcactc ggtattatcg acctggtcta ccccgcacac tggcagccat acattgctca
    22501 ggatctcggt caacaaaccg atatcgatat ctatctcgac cgccacgtag tgcgtcaggg
    22561 gcgatatctt tcgctccatg atgaagtaaa aaactttccg ttacagcact ggctgcgcag
    22621 tacgattatc gctgcgggct cgctactggt gctgtttatg ctgttattct ggatcccgct
    22681 ggatatgccg ctgaaattca ccctctcgtg gatgaaaggc gcgcagacca ttgaagccac
    22741 cagcgtgaaa caactggctg acgctggcgt gcgggttggc gatacattgc gtattagcgg
    22801 tacgggaatg tgtaatattc gcacttccgg tacctggagc gcgaaaacca actcaccttt
    22861 tttaccgttt gactgctcgc agatcatctg gaatgacgcc cgctcattgc cattaccaga
    22921 atctgaattg gtcaacaaag cgacggcatt gaccgaagca gttaatcgcc agctgcaccc
    22981 taaaccggaa gatgaatctc gcgtcagcgc ctcattacgt tcagcaattc aaaaatccgg
    23041 catggtattg cttgatgatt ttggcgacat agtactgaag acagcggatc tgtgttctgc
    23101 caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca gtaaagactg
    23161 ggacgcgctg gtaaaacgcg ccaacgccgg gaagctcgat ggcgtgaatg tgttattacg
    23221 cccggtgagt gcggaatcgc tggataacct ggtggcaacc tccaccgcgc cgtttatcac
    23281 gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg gattcctgat
    23341 tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat cactttacga
    23401 ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc tgatgcatac
    23461 gccgtttaac gccgaaggta tcgtcacaaa aatcttcact gacgccaatg gtacgcagca
    23521 tataggcctt catccgatcc cggatcgttc cggcctgtgg cgctatctca gcaccacgct
    23581 gctgctactg acgatgctgg gtagcgccat ttataatggc gtacaggcct ggcgtcgtta
    23641 ccagcgtcat cgtactcgca tgatgaagat tcaggcctat tatgaaagct gcctgaaccc
    23701 gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg caggttatgc
    23761 taccctgtcg cgcaaattgc ttcactctgg agatttccct catgcatatc aacattgcct
    23821 ggcaggacgt agataccgtt ctgctggata tggacggcac gttgctcgac ctcgccttcg
    23881 ataattattt ctggcaaaag ctggtgcctg aaacatgggg cgcgaaaaac ggggttacgc
    23941 cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat acgctaaact
    24001 ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg atgaccaccg
    24061 agatgggacc gcgtgccgta ctgcgtgaag ataccattcc gtttcttgag gcactgaaag
    24121 ccagcggtaa gcagcgaatt ttgctcacca atgcgcatcc gcacaacctg gcggtaaaac
    24181 ttgagcatac cggtctggac gcacaccttg atttattact ttccacccac acatttggtt
    24241 atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt ctgaaagctg
    24301 aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg caatttggta
    24361 ttcgttactg cctcggcgtg actaatcctg actccgggat tgccgagaaa cagtatcaac
    24421 gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga aggagacgcc
    24481 atgaaagaaa aacctgctgt tgaggttcga ctggataaat ggctatgggc tgcccgtttt
    24541 tataaaaccc gcgcgctggc ccgtgaaatg attgaaggcg gtaaggtgca ttacaacggg
    24601 cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct gcgccaggga
    24661 aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc cgccagcgag
    24721 gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa aatggcgctg
    24781 gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga caaaaaagag
    24841 cgccgcgacc tgttacgatt taaacacggc gacagtgaat aactgtcacc tgcaagagag
    24901 atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaactt tgccgtgcgc
    24961 ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaatca cgattatccg
    25021 cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt aaccgctacg
    25081 ctgaagtttg atggtgatat caccgtacag ctgcaaggcg acggtccgat gaatctggcg
    25141 gttattaacg gtaacaacaa ccagcagatg cgcggtgtag cgcgcgtgca gggagaaatt
    25201 ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat caccatcacc
    25261 ccgagcgaag gtgaacgcta tcagggcgtg gttggtctgg aaggtgatac cctggcggcc
    25321 tgcctggaag attactttat gcgttctgaa cagctgccga cgcgcctgtt tattcgcacc
    25381 ggcgacgtag acggcaaacc ggctgcaggc ggaatgttgt tgcaggtaat gcctgcgcaa
    25441 aatgcccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat caaaaccgaa
    25501 gaattgttga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga agaagaggtg
    25561 acggtttacg atccgcagga tgtggagttc aaatgcacct gttcgcgtga acgttgcgcc
    25621 gacgcgctaa aaacgctgcc ggatgaagaa gtcgatagta tcctggcgga agatggcgaa
    25681 attgacatgc attgcgatta ctgcggtaac cactatctgt tcaatgcgat ggatattgct
    25741 gaaatccgca ataacgcctc tccggcagat ccgcaagttc attaatgagt atgtccggca
    25801 gtgagggctc tgccggattt tacgacttaa tggcctgatg cgctgcgctt accaggccta
    25861 tgaaataaat cgcaaattat tttggtcaga tacagcgtcg tatccgttaa aagcgttgtc
    25921 acttacttaa gtttatttcc agcgtttcac catctaccgt cagatcgcgc cacgctttcg
    25981 gcctcgtccc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    26041 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    26101 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    26161 gatcttgctc gaccaggacg caggcgtctt tttcaatgca ggtcatcacg ttatagcgat
    26221 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccaggg gcaattaaca
    26281 cattttcggc taatactttt tccgatatct gttgctgaag atgttgctct ccatctaacg
    26341 ccatcaacag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    26401 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catatagaga cttacctggt
    26461 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    26521 tgtccagcac cggagaatta agaagcacca gtattaccaa caccaataac gaaaccgcaa
    26581 ggttcacttt gccctgaaga acaagggggt tttgcccttt acgccagacg atactgacaa
    26641 aataccccag cgaccatacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    26701 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    26761 gcggagcagc cagcagagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    26821 acgatttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtca
    26881 acagcaaccc ggcggcggaa atgtggcgag aaatcgcgct caggcccgta aacggcaggg
    26941 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattaacg
    27001 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccgcca
    27061 gtgcggtaac cagacctaac gtgagataaa taaaccagtc ggttgcaaaa aagagcgttt
    27121 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    27181 tcaggccatt ggcgagaaaa atcaccagta atataagtac gttatgccat actgactggt
    27241 aaaaatagcc atagcggcta ctgtcattgc ggatgcgaag gctttgttgt atccacggta
    27301 gcaacaacat tgccatcagc agcagatagc agccaaaatc ccaaagcgcc ttttctgctc
    27361 tccaggggtt catgccatca atttgccact ttagccaacc gctcatccct agcgtggcaa
    27421 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaggagatc acggagaaga
    27481 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgagatttt
    27541 ttccggcaag ccaggcgatc agtaggtagc aaaccagacc ctgaagtaat ccggtagcaa
    27601 tcatccccca gcgtgttgcg ggtgaaagtt caacgttatc cacgttagtt atccagaatc
    27661 aaaaagtggg ttaattatcg catccgggcc gtagtatttt gcttttttca gaaaataatc
    27721 aaaaaaagtt agcgtggtga atcgatactt taccgattga atttgcatca atttcattca
    27781 ggaatgcgat tccactcaca atattcccgc cacataaacc aagatttaac cttttgagaa
    27841 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    27901 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    27961 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    28021 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    28081 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    28141 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    28201 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    28261 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    28321 aacctggcag catcttaaaa gcctggtgac caaacagctt tccggcaaac gtctgttcgt
    28381 tgtcgatgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    28441 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    28501 ggcaggtttc aaaccagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    28561 gaaagaacag ggtctgaatt ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    28621 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    28681 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    28741 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    28801 cccaaaacgc cgcctgattg gcgatgacga gcacggctgg gacgatgacg gcgtatttaa
    28861 cttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    28921 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    28981 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc cgatctatca
    29041 catcgaaaac atcgttaagc cggtttccaa agcgggccac gcaactaaag ttatcttcct
    29101 gaccgcagac gcctttggtg tgctgccgcc ggtttctcgt ctgactgccg atcaaaccca
    29161 gtaccacttc ctctctggct tcaccgccaa actggctggt acagagcgcg gcatcaccga
    29221 accgacgcca actttctccg cctgcttcgg cgcggcattc ctgtcgctgc acccgactca
    29281 gtacgcagaa gtgctggtga aacgtatgca ggcggcaggc gcgcaggcgt atctggttaa
    29341 caccggctgg aacggcactg gcaaacgtat ctccattaaa gatacccgcg ccattatcga
    29401 cgccatcctc aacggttcgc tggataacgc agaaaccttc actctgccga tgtttaacct
    29461 ggcgatccca acagaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    29521 cgcctctccg gaacagtggc aggaaaaagc tgaaacgctg gcgaaactgt ttatcgacaa
    29581 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaagct
    29641 gtaatcagct gaaatctcat tgacccctta tccaaacgga taaggggttt ttttatatct
    29701 aattagctac caatatgtac acggtacact tataattatc aatagaccgc aggatgcatt
    29761 atgttcacgt ttattgaact acaaggattt agcaaacgcc gccagttatt gttacctgat
    29821 gatgagttcc gggcttttca ggaactactc attcaagatc ccactgccgg tgacattatc
    29881 gcaggcacag gcagttttag aaaaattcgc tggaaccgtt ctggaatggg gaaacggggt
    29941 ggcattcgga taatttatta caacgtaaca cgcaatggcc gaatttatct ggcgttgatt
    30001 tatcctaaaa atgaacagga tgatttaaca gaagaacaaa gaaaggcatt gaaactattg
    30061 tccgagaagc tgctttaatg ttgaagctcc acaaattcca ccatggccga aatgccaacg
    30121 gaggcattga aaacagatga aagacgaatt atttgctgat cttcttgcca gcgcagaaga
    30181 aatggtacgc atcgaaaagg gtgaagaagc gcctgaacca gagcacgtcc atacttttag
    30241 cgaaatcgat gtaaaagcga tccgtgaagc aacgggttta cgtcagcaag attttgctgt
    30301 cgccgtaggg gtaagttatg acctggtcaa aagctgggaa accaaacgcc gtcaacctac
    30361 cggagcaccc cgaaaattgc tcctgctttt gcaagcgaat cctttcatta tcaatcaatt
    30421 gaaaacaatc taaaaacagg aggcaccttc gcctcccgtt ttatttaccc ttcttttgtc
    30481 gcaccctgcg cccgcgttac cggcacaggc agccaggcgc gaatggaaag cccgccccgt
    30541 tcgctggtgc caagctccag catcccgtta tggttatcca cgatacgctg cacaatcgcc
    30601 agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc cgcgaacaaa cggctggaac
    30661 aggtgcttac gttgttccgg cgcaattccc ggaccgtcat cttccacctg gaaccaggcg
    30721 cgattcggct ccgttccgct gctgactttg atccagccat tgccgtaacg ggcggcgttg
    30781 accaccatat tcgccaccgc acgtttgatc gacagcgggt gcattttcac ctcaatgctg
    30841 ccggggtaaa gcgcggtttc aatttcccgc tcatagccac tttcggcggc aatcacctca
    30901 ccgagcactg cgttgagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag
    30961 tcgataaact gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc
    31021 aggtagccat cctgttcgct catcatctcg gtagccagac gaatgcgcgt cagcggcgtg
    31081 cgcagatcat gacttacccc cgccatcagc agcgtgcgat catccgccag ttgcttaaca
    31141 ccagccgcca tatggttaaa ggcgcgggta acggaacgca cctccgaagc gccatattca
    31201 cgcagcggtg gcggtataat ccctttaccg acctgcaaag cagcatgttc gagatcaacc
    31261 aacggtcggt tctggatacg aataaacaac cacgccccgc ctatcgccaa tagcataatc
    31321 gccagcgtat agcggaacag cggagaaaaa tcgccctgat gaatttcggt cagcggcacg
    31381 cgcacccaga tattgggcga cagccaggtt ttcagccaga cgacaggcga acttttgttg
    31441 acctcaacgc gtacttccgt cgggccgccc agttgctgcg ccatctgatg gcttaagaat
    31501 tcatagtgtt gtgcccaacg cagacctgcc tcttcagcag cttcgttgga gtagagagag
    31561 atccccagct cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtaccg
    31621 tcctccagtt gcagtttgtc ggtcatcaac atacgcactt cgtacgcgag gactttatta
    31681 aactgctgga ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg
    31741 ctgacgaaca gcaaggtgac gatgagcaat aacgtacggg caaatgaact tcgtggcgag
    31801 aagcgcaatc gcctcatgct ttagaaccgt ccggtacaaa gacgtagccc agaccccaga
    31861 cggtctgaat gtaacgcgga tgcgccggat cttcttccac catgcggcgt agacgcgaaa
    31921 tctgcacgtc gatggagcgt tccattgcgg agtattcacg gccacgggca aggttcatca
    31981 gcttatcgcg ggagagcggc tcacgcggat ggctgaccaa tgccttcagt accgcaaact
    32041 caccgctggt gagcggcatc ggctcatctt cgcggaacat ttcacgcgta ccgaggttaa
    32101 gtttgaactt accgaaagca attaccgcct cttcctgtga cggcgcgccc ggcagttcgt
    32161 tggcctgacg acgcagcacc gcacgaatgc gggccagcaa ttcacgcggg ttaaacggtt
    32221 ttggaatgta gtcgtcagcg ccaatctcca ggccaacgat acggtccact tcttcccctt
    32281 tcgccgtcac cataatgatc ggcatcgggt tgctctggct acgaagacgt cggcaaatcg
    32341 acaagccatc ttcaccaggt aacatcaaat ccagtaccat aagatggaaa gattcacgag
    32401 tcagcaggcg atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg
    32461 tgagataacg ttccagcagc gcgcgcaggc gcatatcgtc atcgaccacc agaatcttgt
    32521 agttctcttg cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt
    32581 aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca
    32641 aagcatatta aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt
    32701 tgatatgatc acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag
    32761 atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat
    32821 ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc
    32881 gaccgcagcg aaaatgctga ctatcagtat aataaaaagc gcctgcgtga aatcgaccgt
    32941 cgcgtgcgct atctcactaa atgcatggaa aatctcaaaa tcgtcgatta ctccccgcag
    33001 caggaaggca aagtcttttt tggtgcgtgg gtggagattg aaaacgacga tggcgtgact
    33061 caccgtttcc gtattgtcgg ctacgatgaa atttttggtc gtaaagatta catctctatc
    33121 gattccccga tggctcgcgc attgttgaaa aaagaggtgg gcgatttggc ggtggtgaat
    33181 acccctgccg gggaagcgag ctggtatgtt aatgctatcg agtacgtgaa accgtaagga
    33241 agagtcttaa cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc
    33301 ctgatttttg atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt
    33361 attgcgggtg aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgac
    33421 gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat
    33481 gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga
    33541 cgtcaggcga tcctcaaatc catttccgag caaggcaaac tcactgacga gctggcgaat
    33601 gccatcaacg ccaccttaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct
    33661 aaacgccgca cccgcgggca aatcgccatt gaagcagggc ttgagccgtt ggccgatctg
    33721 ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg cacaatatat tgatgccgat
    33781 aaaggcgtgg cagataccaa agccgcgctg gacggcgcgc gctatatcct gatggaacgg
    33841 tttgccgaag atgccgcgct gctggcaaaa gtacgtgatt atctgtggaa gaacgcgcat
    33901 ttagtttcta cggtggtgag tggtaaagaa gaggaagggg cgaaattccg cgactatttc
    33961 gatcatcacg aaccgctgtc aacggtgcct tctcaccgcg cgctggcgat gttccgtggt
    34021 cgtaacgaag gcgtacttca gctttcgctg aatgccgatc cgcagttcga tgagccgcca
    34081 aaagagagct attgcgagca aatcattatg gatcatctcg gcctgcgcct gaacaatgcc
    34141 ccggcggata gctggcgcaa aggcgtggtg agctggacgt ggcgcatcaa ggtgctgatg
    34201 catctggaaa ccgaactgat gggcaccgtg cgcgaacgcg cggaagatga agcaatcaac
    34261 gtctttgccc gtaacctgca cgatctgctg atggcggccc cagccggact gcgtgcaacg
    34321 atgggcctcg atccgggtct gcgtaccggg gtaaaagtag cggtggtcga tgcaactggc
    34381 aaactggtgg cgaccgatac catttacccg cacaccggac aagccgcaaa agcagcgatg
    34441 accgttgctg ccctgtgtga aaagcataat gttgaactgg tggcgatcgg caacggtaca
    34501 gcttcccgcg aaactgagcg tttctatctc gacgtgcaga agcagttccc gaaagtgacc
    34561 gcgcagaaag tgatcgtcag cgaagctggc gcgtcggttt actcggcttc cgagctggca
    34621 gcgcaggagt tcccggatct cgacgtttcg ctgcgtggcg cggtgtctat cgcccgtcgt
    34681 ttgcaggatc cgctagcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag
    34741 tatcagcatg acgtcagcca gacgcagctg gcccgcaaac tggatgcagt agtagaagac
    34801 tgcgtaaacg ccgttggcgt cgatctcaac actgcttctg ttccgctgtt aactcgcgtg
    34861 gcgggcctga cgcgcatgat ggcgcaaaac atcgttgcct ggcgcgatga gaacgggcag
    34921 ttccagaacc gtcagcaact gttaaaagtg agccgtctgg ggccgaaagc cttcgaacag
    34981 tgtgcgggct tcttgcgcat taaccatggt gataacccgc tggacgcctc taccgttcac
    35041 ccggaagcct atccggtggt ggaacgcatt ctggcggcga cgcagcaggc actgaaagat
    35101 ctgatgggta acagcagcga gctgcgtaac ctgaaagcgt ctgactttac tgatgagaaa
    35161 ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg aaaaaccagg tcgcgatccg
    35221 cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa
    35281 ccgggtatga tcctcgaagg cgcggtgacc aacgtcacca actttggcgc gtttgtcgat
    35341 attggcgtgc atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa
    35401 gatccgcata ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct cgaagtcgat
    35461 cttcagcgta aacgtatcgc cctgactatg cgtctggacg agcagcctgg cgaaaccaac
    35521 gcccgtcgcg gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccgcgt
    35581 gggcgtgaag cgcagcctgc cggtaacagc gcgatgatgg atgcgctggc ggcggcaatg
    35641 ggcaaaaaac gttaaacgcc ctgatagaca aacgtaaacc tgccagataa agcgttcacg
    35701 ccgcatctgg caacgaacgc cttgcctgac ataaaagtgc cggagaatat ctccggcctt
    35761 tttattccac aaccaaactc tcaatatatc ccgttaatat ttatcacttc attaacaact
    35821 gaaaccttaa ttaaacatta accagtcctg gtaattcgct attcgaatta tattttcgct
    35881 gcgatataac cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc
    35941 acacatttta agtattgctg atagaaacca ttctcattat cattgtgttg ttgattattt
    36001 aatctctcct tcgttggcaa atcatctggt ctcatgtcgc tgtcaaacgc cccatgaggt
    36061 agttatccag ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg
    36121 gaaaatcact ggcttttccc gtgaaatcag cccggcatat cgccaaaaac tgctttctct
    36181 aggcatgtta cctggctcct cttttaatgt ggtgcgcgtc gctccactcg gcgaccccat
    36241 tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga
    36301 agtggaagcg gtttcctgtt aatacggtga taacaacaat gaaaaaatta accattggct
    36361 taattggtaa tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc
    36421 agcgtgtagg taactgggct ggtgttaccg tcgaacgtaa agaagggcaa ttctccacca
    36481 ccgatcatca ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat
    36541 cgcagacctc gctcgatgag caaatcgcct gtcactatat tctgagtggc gacgccgacc
    36601 tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac
    36661 tgctggaact cggcattccc tgcatcgtgg cactgaacat gctcgacatt gccgagaagc
    36721 aaaatattcg tattgaaatt gatgctctgt cggcgcgtct tggctgcccg gtgatcccgc
    36781 tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag
    36841 ctaacgagaa tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac
    36901 tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa
    36961 tgctggaagg cgatatctac agccgcgcct acgccggtga agcgtcgcag catcttgatg
    37021 ccgccctcgc ccgtctgcgt aatgagatgg atgatccggc gctgcacatt gccgatgcgc
    37081 gttaccagtg cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca
    37141 gccgtttcac cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt
    37201 tcctctttgt gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc
    37261 cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca
    37321 cgctccactt cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta
    37381 acaccgtgct gccactggtg ccgcagatcg gcatgatgta cctgttcctc tccttccttg
    37441 aggactccgg ctatatggcg cgtgcggcgt ttgtgatgga ccgtctgatg caggcgctgg
    37501 gcttgccggg gaaatccttt gtaccgctga tcgttggttt cggttgtaac gtaccgtcgg
    37561 taatgggcgc acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac
    37621 cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt atttgcggct gccttcttcg
    37681 ggcagaacgg tgcgctggcg gtcttctcgc tgtatatgct cggtatcgta atggcggtgc
    37741 tgacgggcct gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg
    37801 agctgccggt ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc
    37861 tgaaaggctt cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagtg
    37921 ctttcaacag cttctctctg agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg
    37981 cttccgtcag ccgggtgatc accccagtct tcaagccgat tggcgtgcat gaagataact
    38041 ggcaggcaac ggttggcctg tttacaggcg caatggcgaa agaagtggtg gtgggtacgc
    38101 tcaacaccct ctacaccgca gaaaatattc aggacgaaga gttcaatccg gcggaattta
    38161 acctcggtga agagctgttc agtgcggtag atgaaacctg gcagagcctg aaagacacct
    38221 tcagccttag cgtactgatg aacccgattg aagcgagcaa aggcgacggc gaaatgggta
    38281 ccggggcgat gggtgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc
    38341 tgattttcgt cctgctgtac gtaccgtgca tctcggtgat gggtgctatc gcccgtgaat
    38401 caagccgtgg ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcgctgg
    38461 caacattgtt ctatcaagtc gccagctaca gccagcatcc aacttacagc ctggtgtgca
    38521 ttctggcggt tatcctgttt aacatcgtgg ttatcggtct gctgcgccgc gcgcgtagcc
    38581 gggtgaatat cgaactgctg gcaacgcgca aatcggtaag cagttgctgc gcagccagca
    38641 ccaccggtga ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg
    38701 gggccgtatg gaagcggccc agataagcca ggcattgaat acgccgcagc caatgattaa
    38761 cgccatgctg caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga
    38821 cggctgcctc tctggcagtt gtaaaagctg cccggaaggc aaagtctgtc tgcgcgagtg
    38881 gtgggcgctg cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca
    38941 tccgggcttt tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg cgacgtaagt
    39001 tcctctgaaa gcgtctcgca gggatgaaaa ctggctaatg cacaggtgtg gagtggcgcg
    39061 tagagtcgcg gcattcaaat aacaggtgaa ggaacgccat gagcaaaaag cagagttcca
    39121 ccccacacga tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc
    39181 ttgcgtttca tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg
    39241 agtcgagcag ctttattgat gacgatctgc gtgaaagcta ttccgacgtg ctgtggtcgg
    39301 tgaaaacgga acaagggcca ggatacatct attgtctgat tgaacatcaa agcacctcaa
    39361 acaaactgat cgcatttcgc atgatgcgct acgccattgc cgcaatgcaa aatcacctgg
    39421 atgccggata caaaaccttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa
    39481 gcccctatcc ctattcgctg tgttggcttg attgtttcgc cgatcccaac ctggcaaggc
    39541 agctttatgc ctctgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca
    39601 tgcagcaccg acgtatggca ctgctggagt taattcaaaa acatattcgt caacgcgacc
    39661 tgatggggct ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac
    39721 aaatcaaagg gctgtttaat tacatactgc aaactggcga tgcggtacgt tttaacgatt
    39781 ttatcgacgg tgtggccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg
    39841 aaagattgcg gcaggagggg tttcagaaag gtatacgtac caacgcccta aatattgcca
    39901 aaaccatgct ggatgccgga gtctctcttg aggacgttct gcgatttacc agcctgacgg
    39961 ttgaagacct tgctgaaata aatcatcaat aacgtaaagg atggttttaa tgacagatgc
    40021 agaaagagaa tatcttatta gttacctgat tggttattta ttggatcgac gcctggaagc
    40081 cctggatatc gccaaaagaa tgctgtaatc cggagtccat cttgcagaca ccctgcgttt
    40141 taccggggtg tcagaagaag agttggctgc ggcgagtcag taaagttctg tctcgccatt
    40201 tcaaaagcca cctacaccct ctgcttcaac gccaccagca tgcgacaaaa ctcggccgga
    40261 tgcgaaataa acggcgcatg ggccgctttg gcgaagatgt atgattcgct gtgaggccaa
    40321 agtttatcca gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat
    40381 cgcaaaaacg gcatggacac gttttgcagc gactgacgga gatcgaccgt tttcaggatt
    40441 tccagcccgc cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc
    40501 agcgcccgcg catcctggcg ggccgtttca gtacccatgg tttgtaacgc caggaaccgc
    40561 tccactgtac gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt
    40621 tttatccccg gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc
    40681 gcctgaacac gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc
    40741 cagcctaacc aaatggcttt atcaggtgcc tgtcgcagca cggcttcggc catatcagca
    40801 agtgacatcg caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc
    40861 gtaaaatgcg agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat
    40921 ccgtgcaaca gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc
    40981 atccgttatt gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta
    41041 tgctggctat gccgaatgcc gctggcgtta ggtcattggg ggatttgttc ggtctgctca
    41101 cgcgccgccc gtagcgataa aacgttatgc ccacaatgtg gattacccgc tacacacgcc
    41161 catcttccct gcggccgctg cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt
    41221 gccgactatg cgccgccgtt aagtccgctt atccaccagc ttaagttttc ccggcgcagc
    41281 gaaatcgcca gcgccctggc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc
    41341 actggcttgc aactgccgga tcgcatcatc agcgttccgt tatggcagcg acgtcactgg
    41401 cgtcggggat ttaatcagag cgatttgctg tgtcagccgt tatcacgctt gttgcactgc
    41461 cgatgggata gcgaagccgt cacacgaaca cgggccactg cgacccagca ttttctcagt
    41521 gcccggctgc gcaagcgcaa cctgaaaaat gcctttcgcc ttgaattgcc tgtgcaaggt
    41581 cgccatatgg tgatagtgga tgatgtcgtc accaccggaa gtaccgtcgc agagattgcg
    41641 cagctgcttt tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg
    41701 tagagcctcg atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac
    41761 tatgatccgt atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga
    41821 agaagggaca caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg
    41881 cgtttcttat tgtccgccgg acgctgtaga agccaccgac actgccctga aatttgacct
    41941 gctgaccgcg tatgttgatg agttaagcgc tccttacctg gaagatgcag agatcgactt
    42001 tgttacagac cagttgggtt cccagttaac gctgaaagcg cctaacgcca aaatgcgtaa
    42061 agtcgcagac gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa
    42121 cccacagctt gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta
    42181 cgccattctg caatttggcg gcggctgtaa cggttgttcc atggtagatg tgacgctgaa
    42241 agaagggatc gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct
    42301 caccgaacac cagcgcggcg agcactccta ctactaagtt atcccctctt ttgtagattg
    42361 cccgatgcga cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggcg
    42421 cagcattgct ttggcaaatc cctcccttcg tttcctcgcg ataatatgac caacctctca
    42481 taatttaaat ttaccccgct ctggtgattc tcaaacgccg gatgttaccc gtatcattca
    42541 cgtgggtacc aaacatactc ctgacatctg actacaataa ttagttttgg tgggtatcag
    42601 tcgtggtgcc gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat
    42661 tgacgttacc cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat
    42721 cggtgtaatc ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct
    42781 cgtcctcgtg gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg
    42841 ctccatcaaa gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg
    42901 cgcaatgctg ggcaaaatgc tggcagactg tggtggcgca caacgtatcg ccaccacgct
    42961 gattgctaaa tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt
    43021 tggttttgcc ctgttctatg aagtgggttt cgtgctgatg ctgccgctgg tgtttaccat
    43081 cgcggcttcc gcgaatattc cactgctgta tgttggtgta ccaatggcgg ctgcactgtc
    43141 tgtgacccac ggcttcctgc caccgcatcc gggtccgact gcgattgcca ccattttcaa
    43201 tgccgatatg ggtaaaactc tgctgtacgg tactattctg gcaatcccga ccgtgattct
    43261 cgccggtccg gtttacgctc gcgtgctgaa aggtatcgat aagccgatcc cggaaggtct
    43321 gtacagcgcg aaaaccttca gcgaagaaga gatgccgagt tttggcgtaa gcgtctggac
    43381 ctctctggtc ccggttgtgc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa
    43441 aggtcacgct ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg ctacgctgat
    43501 tgccgtactg attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat
    43561 taacgacacg ctggtttctt ccatcaaaat cattgcgatg atgctgttga ttatcggtgg
    43621 tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat
    43681 gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg
    43741 tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct
    43801 gattgcaacg acgggtgtta gcccggagct gatggttatt gcggttggtt ccggtagtgt
    43861 gattttctct cacgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac
    43921 tatcggcgag accatcaagt cctggtcgat gctggaaacc attatctcgg tgtgcggtct
    43981 ggtaggctgt ctgctgctga atatggtgat ttgataacac cgactgccgg atgcatcgca
    44041 tccggcatca accgcaccct actacttctt cgctgccgct ctgcgccgtc tgtccaaatc
    44101 cttcagcaac ttgttcacgc catcatcggc aaacatcgcc tcaagcgttg cagagagctt
    44161 gcgccgccag tttttatact ggtaactggt gccgggaata tttaccggtt ccgccatatc
    44221 cagccagtct tctggctgta gccctaacag ggcactgtta ctgtcagcga tataacgttg
    44281 caaaccacga ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt
    44341 tggcaggcaa ccatatttat gcagagcatc cagcagtcct tgcttcgcca gttcgcgatc
    44401 ctgatacaga ccgcgcagta ccacttcatc cggatacaac cccagcgttt tgcccagcgt
    44461 tagatccccg ctctcccagt agccgcgtag cgtaggcagg tcgtgtgtcg ccgcaaccgc
    44521 catcgactgc tctgggtacg ctttcggtga gcggaatgtc ttctcgtggt cgttttcgaa
    44581 atagagcact ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg
    44641 tacggtaccg agatcttcac caatcaccat gcagcgatga cgtttacttt cgagtgccag
    44701 aatcgagagc agatcatcca ccggatagtg tacatacgcg ccctgatccg ccgtctcgcc
    44761 atacggtatc caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca
    44821 gttttgcata ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat
    44881 atgcgggtcc attggcggca atccccagtt ctgccccaac gggccgagga tatccggcgg
    44941 tgcgcctacc gacgctttca ggcaatacag ttcacggtcg caccaggttt ccgccccacc
    45001 ttccgctacg ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct
    45061 tatctcccag cagtcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc
    45121 gacgtcatca cgatgttctt cgcagaactg gcgcacttct ggtgaatcca cgttctgata
    45181 catctctggc catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg
    45241 tagtgcgtca aacgccgcct gccagaacag gctgtcgcct tgctcggcaa caaactggcg
    45301 aaacgcggtc atctgttcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc
    45361 tgtcattttt agggcggtaa ccgtggagta atcaacccag tcggcatccc gcgcctgttg
    45421 cagcgtctgt tgcgtggtcg gcaacttcca ccaggcctga gcctcttcgc taagatggaa
    45481 atcttcgacg gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct
    45541 atacgggctg gcactttccg gatttgccgg atagagcgca tgaatcgggt tcagaccgat
    45601 gaacgacccg ccacgttttg ccacatcctc aagcatcgct ttgagatcgc caaaatcccc
    45661 aataccccag tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt
    45721 ttgtttattc agcaacgcct gcggttcgta acagcgtttc ggtgccacaa tcacccggca
    45781 atgcgcgcgc tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg gcagcttcgt
    45841 cggtagattg aacgctttgc cccccgttac atggcctttg tactgcgttc cttcttcggt
    45901 ggtcagcagc cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct
    45961 ggtataaacc atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg
    46021 catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc
    46081 attgatgtaa ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc
    46141 catcgagctt ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg
    46201 atccgagcta aacataccgc agcgggcggt attgaggatc gccgcgcggg tccaggcctc
    46261 ctggtcgcgg tacagcacat ccacctgctt ttgtgcctct acataagcgg cgaaatctgc
    46321 catcaccaga tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc
    46381 atgcttatcg ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac
    46441 cttatctttc ttccgccatt tcaccgggtc gtagcctttg gccagaattg ccttcacttg
    46501 ttccacggta tgaccaaaaa taaagatatt ttcttcaccg actttctcag cgatttcaac
    46561 gttcgcacca tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc
    46621 ggtaccggaa gcttctttac ctgccgtcga aatttgttcg gagatatccg ccgccgggat
    46681 cagtttttcc gccgccgaaa cacaataatc cggcaagaac accaccttca acttgtcgcc
    46741 aaccagcgga tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatgttttt
    46801 cgccaggtag tagcctggtg ctgctttcgc gccgaagagg aagacgcgcg gtacgcgatc
    46861 agcctgcggg ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg
    46921 ctggcgtttg tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat
    46981 ctcaataccg gtacgaactt tcacaaactc tgccagacgg actttattcg cctgcttgat
    47041 ctcgcgatat tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg
    47101 atcgagatcg ttagcccact cttttttcag tgatttatcc aacagagccg ccagtgccgg
    47161 gttgcactgt ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt
    47221 cggccatagc tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc
    47281 cgcaacaccg ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt
    47341 gtcgtgcacc accgccagtt tggcccacac tttttcatcg cccggccagg tattttccac
    47401 cagcgtttta aagcgtgtat taatttcgtt aataatctgc atgtggcgcg gcagtaagcc
    47461 tttcaccagt ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta
    47521 agcgaatgtt ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat
    47581 cagcacgcgc agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac
    47641 ttcataatcc gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc
    47701 cgctaccgaa caggcacact ggaagtattg ctgcatcagg cgcagctttt taccggcagt
    47761 atggttgtcg tttggataga gaactttggt cagtttttcc gcgttgatgc cctgctgttc
    47821 ggcacgcagg aaatcaccat cgttaaattt agtcagatca aacggatgcg catgcgtcgc
    47881 ctgccacaga cgcagtggct gcgccacgcc attacgatag ccgacaacgg ggagatccca
    47941 cgcttgacca gtaatggtaa attccggctc ccagcgtccg tctttcgtca ctttaccgcc
    48001 aattccaacc tgcacatcca gtgcttcatt gtggcggaac cacggatagt taccacgatg
    48061 ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaat gactggcgga acaagccata
    48121 ttgataatta aggccgtagc cagtagctga ctgcccgaca gttgccattg agtcgaggaa
    48181 gcacgccgcc agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag
    48241 caagtcagtc aggttgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc
    48301 gagattcaac aggttgttgc ccgtcagacg accaatcaaa aactccattg agatgtagtt
    48361 aacatgtcgc tgattcgcca ccggtttggc aaatggctga gcacgcagca tttcggccag
    48421 tgcttcactc acagccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc
    48481 ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg
    48541 tgacatagga gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg
    48601 ctcgatgttg accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag
    48661 caaggaaatg tgatctcgac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc
    48721 aatgccatac agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc
    48781 aaaaagcgga aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa
    48841 tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt ttagatagca
    48901 ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac ataagggaaa
    48961 taccgtacaa cccagcattc acgctgtatc agcgtcgttt taggtgagtc attaataaag
    49021 atttggaatt gtgacagagt gcaaattcag acacataaaa aaacgtcatc gcttgcatta
    49081 gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa ataatatttc
    49141 ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaattaa gtcgtccggt
    49201 tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa
    49261 cttccggctg gcgctgatca caagtcctgc gggctacgga aagaccacgc tcatttccca
    49321 gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg ataaccagca
    49381 agagcgtttc gccagctatc tcattgccgc cgtgcagcag gcaaccaacg gtcactgtgc
    49441 gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac tcttcgccca
    49501 gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg atgactatca
    49561 tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc atcaaccaga
    49621 aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg
    49681 tgttcgtgat caactgctgg aaattggcag tcagcaactg gcatttaccc atcaggaagc
    49741 gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa gcagtcggat
    49801 ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct ccgcccggca
    49861 gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg ccagccatct
    49921 ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc gccattttct
    49981 gttgaaaagc gctattttgc gctcaatgaa cgatgcactc atcaaccgtg tgaccggcga
    50041 agaaaacggg caaatgcgcc tcgaagagat tgagcgccag gggctgtttt tacagcggat
    50101 ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggtaacttcc tgcgtcagcg
    50161 ctgccagtgg gaactggcgg cggagctgcc ggaaattcac cgtgctgccg cagaaagctg
    50221 gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct
    50281 gatgctgcgc gatattctgc ttaatcacgc ctggagtctg ttcaaccata gcgaactgtc
    50341 gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa atccgcagtt
    50401 agtgttattg caggcgtggc tgatgcaaag ccaacatcgc tacggcgaag ttaacaccct
    50461 gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc acgcagaatt
    50521 taacgctctg cgcgcccagg tagcgattaa cgatggtaat ccggatgaag cggaacggct
    50581 ggcaaaactg gcactggaag aactgcctcc gggctggttc tatagccgca ttgtagcaac
    50641 ctcagtgctg ggtgaagtat tgcactgcaa aggcgaattg acccgctcac tggcgttaat
    50701 gcagcaaacc gaacagatgg cgcgccagca cgatgtctgg cactacgcct tatggagttt
    50761 aatccagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt gggaaacgca
    50821 ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa tgcatgagtt
    50881 tctggtgcgc attcgtgcgc agctgttatg ggcctgggcg cggctggatg aagccgaagc
    50941 gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac agcttcagtg
    51001 cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg cccgtagcca
    51061 gctgaaccgt ctggaaaacc tgctgggaaa tggcaaatat cacagcgact ggatctctaa
    51121 cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg ccgctgccaa
    51181 ctggttgcgt catacggcta aaccagagtt tgcgaacaac cacttcctgc aaggtcaatg
    51241 gcgcaacatt gcccgtgcac aaatcttgct gggcgagttt gaaccggcag aaattgttct
    51301 cgaagaactc aatgaaaacg cccgcagtct gcggttgatg agcgatctca accgtaacct
    51361 gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc agcgtgtgtt
    51421 gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg tcatcgaagg
    51481 cgaagcgatg gcgcaacaac tgcgccagct gattcaactc aatacgctgc cggaactgga
    51541 acagcatcgt gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc ataaattcgc
    51601 ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc cagaactgat
    51661 ccgcaccagc ccgttgaccc aacgtgaatg gcaggtactg gggctgattt actccggtta
    51721 cagcaacgag caaattgcag gcgagctgga agtggcggca accaccatca aaacgcatat
    51781 ccgcaatctg taccaaaaac tcggtgtcgc ccaccgtcag gccgcggtac aacacgcgca
    51841 gaaactgctg aagatgatgg gatatggggt atgagttcag ccggatatta cgtcggatcc
    51901 ggcttacatc tttatatcat tcaatgctca cccgcgttac gccatcagcc tccaccagac
    51961 taaaccgcac tggcaagaaa cgctccacca ccgcgatatt ggtcagcaga tggcatgagg
    52021 gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggtagcacc agctggtcgg
    52081 cgagatattc ccccactgcc gccgggcttg ccaggtagcg tttcacctct ttcactaact
    52141 gcgcggctac cacttcggca ctgacgcgct tttcaccgac gacgaaaaag cgttcagtga
    52201 tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg cgcggcaggc
    52261 tatgaatatt ctgctcatgc agggaaaaac ttgccaccag cgtagcgatt tcacgctcag
    52321 caacatgcct cggcacgcca gctaatagca cctctccacg catctgcaca atgttcccgc
    52381 gctcgccaag ttgcaaggtg ttaaacaatg ccacgggcga gacttccgct gccaccaccc
    52441 cgcctccagc aggataaaaa ccgtggcgta ataatgtggt ttgctgatga atgcccattt
    52501 tcgccagcag cggctccagc acccgacaga taaaatccgc aggcggtgcc gacgggttat
    52561 cggtgccacc gctcacttca acacgcgaag gtccatcagc aaaccacagc gcgggcagca
    52621 ccgtttgcag taccagcgta caacttccgg cgctaccgat agcaaagcgg taatcgccgc
    52681 cgtgcacggt acctggccgg aagaccagac gctgcgaccc tagctccgcg ccttccaccg
    52741 ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc aacagccccg
    52801 gtttcgcccg cccggcacga atgccagtga tggtaaatgg ctggccagtt atcatcgaca
    52861 ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcgcca tccagcgcaa
    52921 tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta tagataactt
    52981 ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc atcgggattt
    53041 cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga atttgatctt
    53101 ccacgctgaa cagttttttc gctttggtac ggctcattac ccgcccggca ccgtggctgc
    53161 acgaacagaa cgattcttca tttcccagcc cacggacgat aaagcttttc gctcccatcg
    53221 accctggaat aattccatat tgacccgcac gcgcagacac cgcgccttta cgcgtcacgt
    53281 agatctcttc accaaagtgc tgttcttttt gcacatagtt gtgatgacag ttgatctctt
    53341 ccatcgccag cgtttgtggc tgtcttaccg ttttctgcgt aacgctctgc aacgccgtta
    53401 ccacgttttc catcatcgca tcgcggttaa ggctggcaaa aagctgcgcc caggccacgg
    53461 ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga tcacgcgacg
    53521 gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag taagtcccga
    53581 tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg tccgactcat
    53641 caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt ttatagttat
    53701 tggtattcag gaaacgggga tatttttgcg ttaaccactg ataaccggct tccagctcag
    53761 cccatttcgc atcgacgtta acaggcggat tttcccatgc gcctttatca cgtttacaac
    53821 gtccggtagt acgtccgtgc ggcacggctg tttcaatcgc ctgacgcagc tccgccaggt
    53881 tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca caaccgatat
    53941 ccacgcccac cgccgccgga ataatcgtcc ctttggttgg gatcacgcta ccaatggtgg
    54001 aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata aacggcatct
    54061 tcgccgtatt aataagttgc tgacgcgcat cggcctctac cggcacgcct ttggtccaca
    54121 tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt ttctcttttc
    54181 gttggtatgt cctgataatt gcaaccgtcg taccagaaaa ttaaataagc agcttaattt
    54241 tttaattcat tgtttttaaa aaaattatac ctttacgtcc gtaaacggag atttcccgca
    54301 aagccaattt accgataatg aaatatcgtc ttttataagg atatctaaga tgcgtaaaac
    54361 agtggctttt ggttttgtcg gaacggtgct ggattatgcc gggcgcggca gtcagcgctg
    54421 gtcaaaatgg cgtccgacac tctgtctatg ccagcaagaa tcgttagtta tcgatcggct
    54481 ggaattattg cacgacgccc gctcgcgctc gctattcgaa acgcttaaac gcgatatcgc
    54541 cagcgtttcg ccagaaacag aagtggtggg cgttgagata gaactgcata acccgtggga
    54601 tttcgaagag gtttacgcct gcctgcatga tttcgcccgt ggttacgcgt tccagccaga
    54661 aaatgaagat tatttaattc acatcaccac cggcacccac gtcgcgcaga tttgctggtt
    54721 tctactggca gaagcgcgtt acctgcccgc ccggttgata caatcttcac caccgcgcaa
    54781 aaaagaacaa ccgcgcggcg caggtgaagt gacgattatc gatctcgatt taagccgtta
    54841 taacgctatc gccagccgct ttgccgagga acgccaacaa acgctcgatt tcctcaaatc
    54901 aggcatcgcc acgcgtaacc cacacttcaa ccgcatgatt gagcagatcg aaaaagtggc
    54961 gatcaaatcc cgcgcaccga tcctgcttaa cggcccgacc ggggcgggta agtcatttct
    55021 ggcgcgacgc atctttgaat tgaaacaggc gcggcatcag tttagcggcg cgtttgtgga
    55081 aatgaactgc gccaccctgc gtggcgatac cgccatgtcg acgctgtttg gtcatgtgaa
    55141 aggcgcgttt accggggcgc gggagtcgcg ggaaggatta ttacgcagcg ccaacggcgg
    55201 gatgttattt cttgatgaga ttggcgaatt gggcgcagac gaacaggcaa tgctgctgaa
    55261 agccattgaa gagaaaacct tttatccgtt tggcagcgat cgccaggtga gtagcgactt
    55321 tcagcttatc gccggaacgg tgcgcgattt gcgtcagctg gttgccgaag gcaaattccg
    55381 cgaagatctg tacgcgcgga tcaatctctg gaccttcacc ctgccgggtc tactccagcg
    55441 ccaggaagat attgaaccga acctggatta tgaagtggag cgccacgcct cactcactgg
    55501 tgacagcgtg cgttttaaca ccgaagcgcg gcgcgcctgg ctggcctttg cgacatctcc
    55561 ccaggcaaca tggcgcggta actttcgcga actttctgcc agcgtcacgc ggatggccac
    55621 ctttgccact agcggacgca tcactctgga cgtggttgaa gatgagataa accgtctgcg
    55681 ctataactgg caggagagcc gtccctccgc gcttacacaa ctgctgggcg cagaggcaga
    55741 aaacatcgat ctcttcgacc gtatgcaact ggaacacgtt atcgctatct gccgccaggc
    55801 aaagtcgctt tctgctgccg gacgccagct ttttgacgtt tcgcgccagg gcaaagccag
    55861 cgtcaacgac gcagatcggc tacgcaaata cctggcgcgt tttggtctga cgtgggaagc
    55921 cttgcaggat cagcacagct ccagttgaat atgatggtcc gtcagcacct gcatgacgct
    55981 tactggcggc ggggcgtcgg tgtagacagc atcaaccatg ctgatgctcc ccatattgac
    56041 catcgcatta cggccaaatt tcgagtgatc gacaaccagc ataacgtggc gcgagttctc
    56101 aataatagcg cgtttggtgc gaacttcgtg gtaatcgaat tccagcaacg agccgtcgct
    56161 atcgatgccg ctgatcccca gaatgccgaa atcaaggcgg aactgggaga taaaatcgag
    56221 cgtcgcttcg cctatgatcc caccatcgcg gctacgtaat tcgccaccgg cgagaataat
    56281 acggaaatct tcttttacca tcaacgtgtt agcgacgttg agattgttgg tgacaatgcg
    56341 caaattgctg tgattaagca gcgcgtgcgc caccgcttcc ggggtggtgc cgatatcgat
    56401 aaacagcgtc gagccgttgg ggatttgctc cgccactttg cgggcgatgc gctctttttc
    56461 ttcggtctgg gtggccttgc gatcgtgcca cggggtgtta accgaactgg aaggcagcgc
    56521 cgcgccgcca tgatggcgca ggatcaggtt ttgctctgcc agctcattga ggtcacgacg
    56581 aatagtctgc gggctgacgg agaagtgctc caccagttcc tcggtactga cataaccttg
    56641 ctgtttaacc agttcgataa taccgttgtg acgttgtgtt tgtttcattt ataaatccct
    56701 ggaattattt tcgttttcgc gcattgagcg aatcaacaaa agccatcgct aaacccacgg
    56761 ctaacccggc gatgtgtgct ccgttcgcca tcgacatccc aaacaaatca aaccatccgg
    56821 cgacaatcca gatcagcgca aagataatta acccacgttg caggtaaatg ccactttgcg
    56881 gatcgcgttc gccacgtagc cagacgtagc ccatcagcgc atacaccacg ccagaaagcc
    56941 cgccaaacca cggcccgctg aatttttgct gcacatagcc gcttaacagg gcgctaatga
    57001 gagtaatgac aattagctta ccgctaccga ggcgtttttc caccgcaccg ccgagatacc
    57061 accaccagag caggttaaag aggatatgca tcagcgagaa gtgcattaac gcgtgggtga
    57121 agtaacgcca gaactcaaat ttcagtgttg gatcgaatgg ccaggccagc cataacatca
    57181 cttcctgatc gccgagaatt tgcatggcaa taaacaccac cacgcaggcg atcatcatca
    57241 cccaggttac cggacctgcg cgttcacgca aggcagcaaa gaaaggataa cggcgataat
    57301 gcaggccact gccggtatgg cccgcctgcc agctggccgc cagataacgc gaatctgccg
    57361 ggttttcgag aaaacgcgcc agctccgccc gtacgcgctc ggcctgggac tcatccgcca
    57421 gccagacgtc gctttggtta tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt
    57481 aatcaacaaa cgcctgcgcc acgcgggggt tagcaaaaga ggtaatcatc aacattgttg
    57541 ctgtcgctta ttccacacaa aaggggacag tataaagcgt tacgcgccgt acgccacctc
    57601 tgcgggaaac tgtcgctgcc aggcttcaaa gccgccgtca atgctataga ccacatcgta
    57661 gccctgttgc agcagatact gcgccgcccc tttgctgcta ttgccgtgat aacacatcac
    57721 catcaccgga gtgtcaaagt cgttatcacg cataaacgcg cccagtgtgt cgttggttaa
    57781 atggaaagcc tgcaccgcat gccccattgc gaaactctgt ggatcgcgaa tatcgaccag
    57841 caccgcctct ttttcctgca acttctggtg cgcgtcggca acgttaatac attcgaactg
    57901 atccatgcgt ctctctttct ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg
    57961 aagcggcaga taaacgccat aatgttatac atatcactct aaaatgtttt ttcaatgtta
    58021 cctaaagcgc gattctttgc taatatgttc gataacgaac atttatgagc tttaacgaaa
    58081 gtgaatgagg gcagcatgga aaccaaagat ctgattgtga taggtggcgg catcaatggt
    58141 gctggtatcg cggcagacgc cgctggacgc ggtttatccg tgctgatgct ggaggcgcag
    58201 gatctcgctt gtgcgacctc ttccgccagt tcaaaactca ttcacggtgg cctgcgctac
    58261 cttgagcact atgaattccg cctggtcagc gaggcgctgg ctgaacgtga agtgctactg
    58321 aaaatggccc cgcatatcgc cttcccgatg cgttttcgcc tgccgcatcg tccgcatctg
    58381 cgcccggcgt ggatgattcg gattggtctg tttatgtacg atcatctggg taaacgcacc
    58441 agcctaccgg gatcaaccgg tttgcgtttt ggcgcaaatt cagtattaaa accggaaatt
    58501 aagcgcggat tcgaatattc cgactgttgg gtagatgacg cccgtctggt gctcgccaac
    58561 gcccagatgg tggtacgtaa aggcggtgaa gtgcttaccc ggactcgtgc cacttctgct
    58621 cgccgcgaaa acggcctgtg gattgtggaa gcagaagata tcgataccgg caaaaaatat
    58681 agctggcaag cgcgcggcct cgtgaacgcc accggcccgt gggtgaagca gttcttcgac
    58741 gacgggatgc atctaccctc accttatggc attcgcctga tcaaaggcag ccatattgtg
    58801 gtgccgcgtg tgcatacaca aaagcaggcc tacattctgc aaaacgaaga taaacgtatt
    58861 gtcttcgtga tcccgtggat ggacgagttt tccatcatcg gcaccaccga tgtggaatac
    58921 aaaggtgatc caaaagcagt aaaaattgaa gagagtgaaa tcaattacct gctgaaagtg
    58981 tataacacgc actttaaaaa gcagttaagc cgggacgata tcgtctggac ctactccggc
    59041 gtgcgtccgc tgtgtgatga tgagtccgat tcgccgcagg ctatcactcg tgattacacc
    59101 cttgatatcc atgatgaaaa cggcaaagcg ccgctgctgt cggtattcgg cggtaagttg
    59161 accacctacc gcaaactggc ggaacatgcg ctggaaaaac tgacgccgta ttatcagggc
    59221 attggcccgg catggacgaa agagagcgtg ctgccgggtg gtgccattga aggcgaccgc
    59281 gacgattact cagctcgtct gcgccgtcgt tacccattcc tgaccgaatc gctggcacgt
    59341 cactacgcgc gcacttacgg cagcaacagc gagctgctgc tctgcaatgc gggggcgata
    59401 ggcgatctcg gggaagattt cggtcatgag ttctacgaag cggaactgaa atacctggtg
    59461 gatcacgaat gggtccgccg tgccgacgat gccctgtggc gtcgcacaaa acaaggcatg
    59521 tggctgaatg cggatcaaca atctcgtgtg agtcagtggc tagtggagta tacgcagcag
    59581 aggttatcgc tggcttcgtg atttaacgta aggtggtcag gtcagatttc aatctgacct
    59641 gagactgatg ataaacaaaa aaactgcctg atgcgctacg cttatcaggc ctacgtggtt
    59701 tatgcaatat attgaatttg catgattttg tagacaaaat aaggcgttca cgcagcatcc
    59761 ggcataaaca aagcgcactt cgtcaacaat ctgacctact ttttgattca aggaattacc
    59821 gcaaagccca cgcaatcata cccaacgtaa caaaactcag acacattacc cactcgcccc
    59881 acttcttcct ctgacttcta tttttgctac gccactggca ataatatccc aatgcaaaac
    59941 caagctgggc gcttatcgga ccgataaagt cagtccacgg ctgagaccca aattgaaaaa
    60001 attcaggggt aaccagacaa tggtcagaaa gaaattgcgc atattttcca gcaaagcaat
    60061 ttgcgctggc gcgacataga aaatgatatt ccacaggagc aaccagaaat aaactggtgc
    60121 aaataccaac caacaatgga gtctgctttt cagttcagat tgagctctgc aagaaaattg
    60181 ccaaccaacg caacacatgg cacagaaaaa aaaagtgcat attcagccaa taacccaagt
    60241 tggaattcca gaagtgaaat acgcctgaac atgagcggga tagagaacag tatttctccc
    60301 ataaaactca gcgtgataac acaagcagcc agcgcaacca ccgtcggcca caccaacaac
    60361 ggcacaataa caaaaccaag aattaacgga ggaatataaa gtctgatttt attcatcttt
    60421 attgtcctga tgattcggat gatgcgtcat aaatcggcac atagcccaca tcctctacca
    60481 attgctgtcc ctgcgggctt aaaaaccagt cgacgaattt ctgcgtttcc agcgtagggt
    60541 tttcgtgcgt caccatataa gcatgaatta tgtacggata agtgccgtta cggatatttt
    60601 cctctgtagg tgcaacgcca tttatagcca gcagtttgat ctgtttgcgc attttgagca
    60661 tattgtcatt catacgtgtg acgtaatagt ggaaggtgta tccaatagaa gatggtgtat
    60721 tttggtagtc ggcgacttcg gtaattaaac cgcccataag atcgataaca gtggattttt
    60781 tggctggcag cattggagta tcttgcataa ccagtccctt cataaccgtt tggcttccag
    60841 aatcttctgg ccgctgccag atttttatac cttcgtcacc gccgcccact ttattccagc
    60901 gactcacttt accgctaaaa atgtctttga cctgctgttc gctaagtgaa ttaaccgggt
    60961 ttttgatatc aacgataaag acaaaggctt cgcgggcgaa agcggtatat ttgagtttaa
    61021 cacctgcttc ttctgcacgt tttttctgtc cttccgacgg ttgtgcaaca aaaataaccg
    61081 tcgcggtacc cttaatcaga ctatcgtagg cttcagctgt tcgggtttgt tgcaaaccat
    61141 acgcctgcca ggccagcgag gttcgatcct tatcttttgg ctcgggtact aattcataat
    61201 acgcggaggc atacagcggg tataaggctg tagcgccgta cactctcggc catcttgacc
    61261 aatcatcgtt gtaacgtaat tcaggttgcc cccgcagaga cgtcagtcca tggctgtctt
    61321 cttccctgac gctgttatag tacccttccc ggtcgacaca tcccgccagt agcgccgtac
    61381 ctaatagtaa aacacaggtg aatttattca ttgcgctgat tcctttctct cgagcgttat
    61441 ttcattgttg gatacagcgg cacatagccc acatcttcca ccagactctg cccctgcggc
    61501 gttaaaaacc attcaaccag tttttgtgtt tctgacgtca tattgtcccg cgttaccata
    61561 aatgcatcga cgacataggg atatttgcca ttgcgaatat tttctgcagt cggtgcaata
    61621 ccgttaatcg ctagcagttt tatattttta tcggcattca tttgtgttgc gtaatagcgg
    61681 aaggtgtagc ctattgcgtt gtttgtatta cggtattcgg caacgacttt aatcattccc
    61741 tccatcatgc ttgccacttc ggtttcctgc ggcgagatca tgcggacatt tttcatgacc
    61801 tgcgattgca tcactgtctg gctgccagag tcttccgggc gttgccaggt ctggatctcc
    61861 tgatcgttac cgccaaccgt gcgccagttg gtaattgcgc cactaaaaat gtcacgcact
    61921 tgttgttcag tcagggaatt aaccggatta tccgcattga cgatgaaaac aaacgcttca
    61981 cgggcaaatg gcgtgtacag caaagtgacg cccgatttct cagcgcgttt tttttgccca
    62041 tcggaaggtt gcgccacgaa aataatatcg gcatcaccgt taacgatttt gttatatgct
    62101 tcttgcgtac gagagttatc cagataatcc caaacgtgaa aatcctctgg tattacactt
    62161 aatgcataaa atgcagaggc ataaatgggg tacgccgccg tagcgccatc gagtcgcggc
    62221 cagttttgcg taaattgaat ttgtggttta ccacgcagtg gcgtcagttg attattaagt
    62281 ttatcgggtc gccaggccca ggtatcaata ttttcacgca caccgacgcc gggattaagc
    62341 tgtttatata aataagcctg ataaccagcg acaataccca gtaagagaag aatgaacaga
    62401 agtaaattac ggcaacgtag accggaggtg ccggttaaag ggtgtctggt cggccagccg
    62461 tatcccagtg aaaaactgat atgcgcgcca acgggaataa tgactatcca ccagtactca
    62521 ccaccgacga atgagactgt aaaaacgccc aaccagggaa gcataaggtt tgccaaccaa
    62581 ccggctccca tctggcgaaa ttgataatct gccagatgta gcgtgattat ccagccaacc
    62641 agggttaata aagcgggaac taaaaaaaga cattgtcgcc aggcggctat ttctctgttt
    62701 tctggtgcgc tatcgcccgc ccacactcgc ccagagaaaa atagcagtaa tgcgcccagc
    62761 gtcagcataa agacgttgag tagaagataa attgttggtg taaagagtaa attgcaaaac
    62821 tcaagaatag cggcaagccc ggcccccagc atggcaacaa ccgctgccaa aaattgcgcc
    62881 agtatgggga tgccaaaaaa agtcattacc agcgaggtca aaatccattt tccgttttgc
    62941 attattcttt ccattcttgc tgaatggcga aattatactc cccgataacc ctcgccccct
    63001 ctggacactt ttcctaaata aaggcgaaaa aaaacgggac cttttggccc cgttctgttt
    63061 tttagcgaac ttacaatctc accggatcaa tatgccagat atgatcggcg tactctttga
    63121 tcgtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct tttgcggtcc
    63181 actcttcctg acgctcgtag agttcgtcga ctttatcctg acaatcgaca tagctgcgat
    63241 aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcgacc agatcgcgat
    63301 agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc acctgatgca
    63361 gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc agttcttcca
    63421 cttcttccgc tgtgttacca aagataaaga tattgtcagc accgacatga tccagcatct
    63481 cgacattcgc accgtccagc gtaccgatag tcagcgcgcc gttaagcgca aacttcatgt
    63541 tactggtgcc ggaagcttcc gtccccgcca gcgaaatctg ttcagataga tcggccgccg
    63601 gaatgatcaa ctgcgccagg ctaacactgt agttcgggat gaacacgact ttcagcttgt
    63661 cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa tgaataatgt
    63721 gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg cgcggtaccc
    63781 acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt gatcacgtgc aacacattca
    63841 tcaattgacg tttgtattcg tgaatacgtt taatttgcac atcgaacagc gctttcggat
    63901 ttaccaccac attcagctgc tgggcgatat actccgccag acgctttttg ttctccagct
    63961 tcgcctgatg caccgcgtga ttaaccattg ggaaatcaca gtgttgttgc agctcattaa
    64021 gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcaca gctgaaagcg
    64081 atgggttcgc taccgccagc cagcgacgcg gggtcacacc gttggtaacg ttggtgaaac
    64141 gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcagg ttagagtgca
    64201 gttccgatac accgttaact ttgtggctca ccacaaccgc cagccaggcc atacgcacac
    64261 gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta tcgttcggat
    64321 actgttcctg caaggtttta aggaaatagt cgttgatttc aaagatgatc tgcaggtgac
    64381 gcggcagaat tttacccagc atatcgactg gccaggtttc cagcgcctcg ctcattagcg
    64441 tgtggttggt gtaggagaag acctgacaac acacttcaaa cgcgtcgtcc cagctaaatt
    64501 ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagcaccgga tgggtatcgt
    64561 tgaggtgaat cgcaatttta tccgccaggt tatcgtaggt tttatgcaac tgataatggc
    64621 ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc agtcgcagct
    64681 cacgcccgga gtaggtggag tcgtccggat acagtacgcg agatacgttc tcggagtggt
    64741 ttttatcttc cactgccgcg aagtagtcgc cctggttgaa tttaccgagg ttaatttcgc
    64801 tactggcttg ggcactccac aaacgcaacg tgttggtcgc gtcggtgtcg taacctggga
    64861 ttatctgatc gtaagctacg cccagaatct cttcggtttc aatccagcgc gtttttttac
    64921 cttcctgctg aatgcgaccg ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt
    64981 cccacgggtt accgtattcc agccagtagt caggcgactc tttctggcta ccgttaacga
    65041 tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc ggcaacccta
    65101 acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg ttaccgaggc
    65161 ccgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct tccagtgcgc
    65221 cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggaaagcgta cggccaatca
    65281 aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg gcacggtttg
    65341 aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg ttcagccatt
    65401 catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg taagcgatag
    65461 agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat
    65521 aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac ttcgccgcga
    65581 cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac agtgaaggac
    65641 gagaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca ttactatctt
    65701 caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacaa tcagaaaccg
    65761 tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc aatccataaa
    65821 gttgcgttaa gccgcacggt tcaaaacgac tgggcaccag aatgacgtcc gcgccgccca
    65881 taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg ccggggtatt
    65941 ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg agtagcgcca
    66001 gttgcccgcc ctgctccaga agacccggta aggcctccag caccaggtcg agacctttct
    66061 ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca
    66121 ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg cgggtgtaac
    66181 gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg ccgttcagta
    66241 cgccggaaag acgcccttca cggtgacgct gttgcaacag accttccata ccgtaggcaa
    66301 actgcggttc ggtgatctcg cgagcgtagg ttgggctgac tgccgtaatg tgatcggcat
    66361 agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca tgaatattaa
    66421 agaacgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg ccttgatagg
    66481 ccaggttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc agatacgcag
    66541 gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa
    66601 gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaac acgttgtcgg
    66661 tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag agatgcggcg
    66721 cgtcaatcag gtaaatgcca accccgttgt aatgaccgaa caacagcgtg atatgtccgg
    66781 cgaaggtatc acgacgggat actacctgcg catcggtcac gccacggcga atatcgggaa
    66841 atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc
    66901 caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa catacatgta
    66961 aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc gcgttaccag
    67021 cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt tttcaccaat
    67081 caccatgcct tccggaataa cacaagcacg atcgatgacg cagcggcgca gacggcacga
    67141 gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga atgaattcac
    67201 gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca cacaaccgcc
    67261 ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca cgaatttcgc
    67321 tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca tatccagttc
    67381 cggcaccaca gaggccagat cgaggttcgc tttccagtaa gcttccagcg tacccacatc
    67441 gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga atgggtgtgc
    67501 ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt cgtggctgga
    67561 gttctcatcg cgatcgtctt cttccagcag ttcatacaga tagtcggcgt caaagacgta
    67621 gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggcg ggttagctgg
    67681 tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc caaatgcgga
    67741 ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg
    67801 gtcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca ggatcaccac
    67861 gtattccgct ttataacggc ggataatgtc gaggttttgg gtaactgcat ctgcggtgcc
    67921 gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga caaactcgtt
    67981 catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag tgtgggactg
    68041 gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag acagcgcaaa
    68101 gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct tattggttaa
    68161 atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt tcaatggcag
    68221 ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta actccttttt
    68281 tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc cgtaatcact
    68341 gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg taaaacaatc
    68401 tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag cagaatttgc
    68461 agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata tcgatttagc
    68521 caacggacat tgccgtcgcc ttcttcccac cagcgattct ccaccaaagc gggaatgcgc
    68581 ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag
    68641 tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg ctggctgtga
    68701 ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa cgttgttaac
    68761 agggcgtgaa tgctgtcgcg acgccgttca accaggtcaa gagtaccgcc taacccttct
    68821 ttaccatgat tgttactata attgttgttg gtcccgtcgc gattttcttc tccgtttgct
    68881 tcattatgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg
    68941 acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc gctggaggca
    69001 gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gaagccagaa acgacgggca
    69061 gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc cacctgataa
    69121 ccaccaggag caatatccca cggttcagca attaacttca cctgcgagag caccgggcag
    69181 ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg gcccatgact
    69241 gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata acgcaggcaa
    69301 gcgctggcat aatccactac cgccggatga ctcaaattga gcgtgttgcc acaaccggtc
    69361 cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttgtc gatcccacgc
    69421 agagagaata acggaccgtc gaggtccagt tccgcactat ggttgagcac gatatcaaga
    69481 atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc atccagcgcc
    69541 gtttctgggg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg gttataaccc
    69601 cagtagttac ttagacccat acgttgcagg cgtggttcgc tggcaaactg cgccaccggc
    69661 agcagttcca gcgcggtaat gcccaactgt ttcaaatagt tgatcatcac cggatgcccc
    69721 agggctttat aagtgccacg gatctcgacc gggatctccg gatgcaggta cgttaatcct
    69781 ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg ggcatcatct
    69841 tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc gttgtcgcga
    69901 tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaattc cccgtcaatt
    69961 tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc ctcggcgggt
    70021 tgccaggggc catgaacgcg ataaccataa cgcaaacccg gacgcgcatc cggcagataa
    70081 ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg gccattggcg
    70141 tcaaagatac acagttctac ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc
    70201 tgaccgtcgt aatgcgcgcc gaggggtgcg ggtttaccaa tggcgagttg tgtcattctg
    70261 cctcccgaac cagccaaata gtggccagcg gtggtagtgt caggcttaat gaatgctgac
    70321 gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgccgaca ttactgccgt
    70381 gatagtgcat ggaatcggta ttaaggattt cacgccattt gcccggctgg ttaatgccga
    70441 agcgataatc atgacgcggc accggagtaa agttactggc aacgatgatt tcgttaccct
    70501 ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc accagccatt
    70561 caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg taggtgaggt
    70621 tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct tccaacagat
    70681 gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg ttccccatga
    70741 acagcagttt cttgcccggg aatgcccaca tccagccgta gtaggcgcgc aggttagcga
    70801 atttctgcca tgcgtcgccc ggcatccggt cgagaatcga ttttttaccg tggaccactt
    70861 catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca
    70921 gtttatcgtg atgatactga cgataaaccg ggtcgagctt catgtagtcc agggtgtcat
    70981 gcatccatcc caggttccac ttgtaccaga agcccagacc gcccatgtcc tgcggacgag
    71041 aaacgccagg gaaatcggta gactcctccg ccattgtcac cgcaccggaa acctgctcac
    71101 caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg cgcccgccaa
    71161 attcgttcgg gatccactcc ccctctttac ggctgtagtc gcgataaatc attgacgcca
    71221 ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc gcgttaccga
    71281 caaggaagtt actgacttca cggcgaccat agttgtagat cagcgtattc cagtcctgat
    71341 gatagccttc gcgcggatcg ctgtgttcat acaagttcgt gccatcaaat tcggcaagcg
    71401 caaagtcatc ggtcgggaag tggcctggca cccagtcgag aatcacgttc agaccagctg
    71461 cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg cgggtcggtg
    71521 catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg ttaatgggca
    71581 gcagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga tcggccagct
    71641 cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggagccc aggtgaactt
    71701 cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca gtctgcacaa
    71761 ccttttccgg cagaccgcaa ataagagacg cggtttccgg gcgcatttgc gcttcgaagg
    71821 cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctca tatttataga
    71881 gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct ttacgcaggc
    71941 gcatcggatg acggcgaccg tcccagtagt tgaattgccc aaccaccgag acccgacggg
    72001 cgtttggagc ccagacagaa aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg
    72061 cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag gcatccattt
    72121 cctggattag cggaccaaaa cggtaaggat catctatcag gttttgctga ccatgccaga
    72181 caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacaccg ctaaagaatc
    72241 cacgggagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt tcaatcaccc
    72301 acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg gttttatgca
    72361 tccccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc
    72421 tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca tccaactgga
    72481 tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc tgtgcatcct
    72541 ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag ccacccttaa
    72601 agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg cttactcctg
    72661 tattacgcac tagcaggggc ggcatcgcgc cccagattta atgaataaag attacgccag
    72721 ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt cgcccacggt
    72781 aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta ccggcgtggt
    72841 cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc gatcgttcgg
    72901 aacgactttc gcccacggat tgtgcgcagc cagcagttct tccacggtcg gaatagacac
    72961 atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac
    73021 acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg tttccgcctg
    73081 ccctttccac tcttcgcggc tctgaccgtt atcaagctgt ttgtcgatcc acggaatcag
    73141 gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac gggttaaggt
    73201 tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt catctgccac
    73261 atggctatac agatggccca tctgggttaa taactcacgc atatgtcgcg caccaccgcc
    73321 ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg cgaataaacc
    73381 acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag tcctgatgcc
    73441 attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga tgatggcgtc
    73501 atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc cgctttcacg
    73561 aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa tgatatcgag
    73621 ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga
    73681 cggcgcagcc tgaccaagct gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc
    73741 gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc agccgataaa
    73801 accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa atgtgccggt
    73861 ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt gaaattaatc
    73921 aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg cagaaagtcc
    73981 gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca gttttattga
    74041 tcctgattat ggatccgctc ggaaacctac ctattttcat gtccgtactg aaacatactg
    74101 aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc ctggtgatgc
    74161 tggtgttcct gtttgcgggt gagaaaattc tggcatttct tagcctgcgg gcagaaaccg
    74221 tctccatttc tggcggcatc attctgtttc tgatcgccat taagatgatt ttccccagtg
    74281 cttcaggaaa tagcagcggg cttccggcag gtgaagagcc gtttatcgtg ccgttggcaa
    74341 ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct catcagtacc
    74401 cgaatcagat gggacatttg gtaattgctc tgctgctggc ctggggtggc acctttgtca
    74461 tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg aacgcacttg
    74521 aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc gacggcattc
    74581 gaatgtggat gaaggggtaa ggttcaaaca ggtgctgcac acaaccagag gcggcacctg
    74641 ttttattacc tgaactgata atgaccttcg cccaatcgga taacccttcc ctcttcttct
    74701 gcgcggttca gccacatact aatttgttcg atggttaaac cagtacaaga cgccagcctt
    74761 tcaatactta tcgattcctt tgcaaggatc gccagttccg ccacaaaaag ttgataaaaa
    74821 tcgacagggg tatgcgaggg catcactgcg taatttgcgt catcttcgtg cagcgaaaac
    74881 aattctggtt gagcgttacg gagtgctctg tcatacatca gcccaggaac gtctggcagt
    74941 ctgatcgtca cattctcagc ctgttcagca ctccatgacg ccccattttc tactaatcgt
    75001 gaattggctg attgcacatc ctgatttggt ttaaccagaa caggcagcca ctgatgtttt
    75061 aacgcctcca tcgctccggt tatcgttcca ccggtcattc ccgcacgtac aaccattgcg
    75121 ctttcagcaa ggcaataaat atatttattc cgcgccatcg cattgccgac ggtgaaacgt
    75181 acctctgggt aaaacggtga aatcaacacc aggttgcctg ctataagccc ttcacgccat
    75241 ttcactaacg tgctcgtttt taacaagcta tcagctaata cgccaacggc agttcctccg
    75301 gcctccagcg ccgacgctat tgcacattca tcaatacctc gcgcaccacc agagataacg
    75361 caaatcccct gttgggccag tttcgctgcc agttgttggg tatagcgcaa atcggcagtc
    75421 ggagcatctc gcgagccaac aatcgccata ccttccgctt gcagtaatgc tttattcccg
    75481 cagccaaata aaacgggagg tgcatccgtt cgcaatcggt ttttcaagcg aacgggataa
    75541 tcggcatctc cgcgggttaa gatccataaa ccggcgcgtt gccacttatc taccgccaac
    75601 gccagactat gaccacgcgc cagcaagcca agaatacgct cctgagaaat acgcggatcg
    75661 ctccagcctg taagtttctc ttgcggttgc ggcaccagaa gatcggcggg actgatacgt
    75721 tggtgcttca gccataatgc aaacttcccc cattcactat tactaagagg tttatactca
    75781 ctcgccgccg cgcgagaaaa atcgctggtt agcaggagag tcgcttgtgc attggctgaa
    75841 agattcatca attttttacc gaggtagaag caagggcaag aggataaacc gtcgggcaac
    75901 ctgcctggcg taacagtgct gtcagaactg tcagcgtcca tgcagaatcc acgatatcgt
    75961 caaccagcaa cgccggacct ggcatcaaag gaggggtaat cacaaacgcc ccgtcgagat
    76021 tttgacactg gtgaaaacgg ttttgctgca ttttctgcgg tggattgtcc acgacttttt
    76081 caatggcatc aataaaaggt aagccaagtt tcgccgccag tcgccgggca aaatcaggaa
    76141 ccaggtcgag gtgccttaat gaaggaacgc agcataccca ggttggttca ggatgcggat
    76201 tccagcgttg gcgaaccatt tccgcacatg cttctaccag ctcatcactt aagcgccccg
    76261 tttttttctc ctgtgctacc tgctttcccc acccggaatc tccccaacga gaaaggattc
    76321 gccccgtaga tccttgtaaa cccgcaggca agttcccttt aaatccgtat tgcgtaaaag
    76381 ccccagctgc cacctgttta ttgagattta acggcaggtc agcgtgttta ataaataaat
    76441 ttgccgcatg gagtaagccg ctatcaatgt cgggcgacaa taacggatgc tgaagacaac
    76501 tgctacattt accacaacgt tcggcacttg gctcatcgag tgcacggcgc agaaattgca
    76561 ttttgcactc cttggtcgtg atatagcttt caacgtctgc cagttcactt tttctctgat
    76621 tcatcaaatg gttaattcgt tcatgatcag gagaaaaact gacgatagtt ctgcgccata
    76681 atttctcggt atacaccaca ggcgatggat tttccgctac caataatttc agtgcttttt
    76741 ctatttgccc ataacgaaga ttcgtccgtt gttcaatgcc tcgtagggta agaccgtcat
    76801 tctcgctaag tacgttgagt atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac
    76861 ggaagaattg atgaatagcg cggtcttcac caccacaaag caatatgcca actgcggaat
    76921 ctatagcacg cccggcacgc cccacctgtt ggtagtaacc gacaatagac cccggcatct
    76981 gataatgaat gacgaaacct aaatcaggtt tatcaaaccc catacctaat gccgtcgtcg
    77041 cgaccagcac tttgatttta tttgccagca gtgcctgctc cagatattcc ctggcagtat
    77101 tagaatcttc cgcgccttca caagtcacgc cgctgtagta agcaaatgca ctgataccat
    77161 ttttccttaa ccactcggcg acaagttcag catcgcgagt cgtcagggta taaacaattc
    77221 ccgatttgga aaactgaggg ataaccgttg ctaaccatgc cagacgggat gactgttctc
    77281 ctaagactaa ggcatcaagc gccaaacttt cgcgagccag cgttccacgc tgaatcacaa
    77341 tgtcacccaa ttgctgacgg atatcctcaa caacacggtt attcgctgtc gcggttgtac
    77401 ccagaacagg ggtattcgca ggcagttggc gcaaaatatc taatatacgt cggtaatccg
    77461 ggcgaaaatc gtggccccaa tcagagatgc agtgcgcctc gtcgaccaca agcaagccaa
    77521 tacgatcggc tataggatat aaaaccgttt cgataaagtc ctggtttgcc aaacgttccg
    77581 gggaaaccag caagcaatcg acctcacctt gcagcaactt atcgctaatg cgctgccatt
    77641 cttccctgtt tgtagaattc aacgtttcag ccgtaatacc taaccgttca gcggcggcaa
    77701 cctggttacg catcagggca agcaaaggag agatgataat tgtcgggcca gcgccgcgat
    77761 cgcggaagat tttactggcg atgaagtaca cggcactttt cccccagccg gtgcgctgta
    77821 caaccagcag ttttcgccgc tgattaacca ctgcatctat ggcttcccat tgcccttccc
    77881 tgaaagtagc ggtggtattt cctaacatcc gctgtaaaag caattcagct ccgtgctttt
    77941 ccatctttat ctcctgcgat ctttaggcag tgattagaac ggattattag caggtaaatt
    78001 tgcgagagag atcaaaaaga tgaagagagg agataagaga aatgaattac tgcgagcaag
    78061 ctcgcagtaa tttgtcgcag taattaactc aacagctgga acgcaatcat cccaacgatg
    78121 gcaccaacag tgccgagtat ggtttccatc atggtccagg ttttcagcgt ttcggcttcg
    78181 gtcgcgccgg taaatttacc gaacaaccag aaaccggcgt cgttaacgtg gctgacaaca
    78241 atcgaaccac cggcgataca aatcgacagc gccgccattt gcgcaccgga gtagttcagt
    78301 tgttcaataa ccggcatcac cagtcccacc gccgttaaac aggcaacggt ggcagaaccc
    78361 tgaatgatgc gcactgcagc tgccagcacg aagcaggtga tggcaatcgg caggcccatg
    78421 ccggttaacg cttcgcccag tgccggacct acgccagagt caaccagcac ctgtttgaac
    78481 acgccgcctg caccaatcac cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa
    78541 atctccatca ctttgtcttt tggcatgccc tgacgcattg ccaggccata aatcgccacc
    78601 agacaagcaa ccagaatcgc ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg
    78661 gtagagcctt ctggcacaaa acgcgcggca atggttttca gccctaccag caccagcggc
    78721 aacaggatca gcgacaggct gaatccgaaa gatggcattt tgccttcgcc gagatgtggt
    78781 tcgctgatgt cgtcaggaat atgcagctcc acgtagcggc tgatgaaatt accccacagc
    78841 ggcccggcaa taatcattcc cggaattgcc gcacacaggc caatcaggat catccagcca
    78901 aagtcggcat tcatctgcga tgccagcagc attggcgctg gtccaggcac caggaacgca
    78961 gccgcggccg ccacgcctgc gaataatggg attaccagct tcaccaggtt cgtaccggtg
    79021 tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa tcgccacttc aaagaacagc
    79081 ggtagcgcac agaccagccc cgcaaggccg atggcataat gcgcgcggct gtgaccgaag
    79141 gatttgagca ttttgacggc aatctgatcg actgcgccgg tttcatgtaa gatcttgcca
    79201 aacatagctc ccagggcgac aaccaccgcc aggaagccga gggtgcctcc catccctttt
    79261 tccatcgtcg ctgcgatttt atcgagcggc ataccggaaa aaaggccagc ccccatggac
    79321 accaccatca aagccaggaa agcgtgcata cgcgccttca tgactaaaaa cagcagcagt
    79381 aaaacagacc ctactgctgt taaaacaagc gttaatgtag tcactactta tttgcctttt
    79441 ttaataacct caatggtgct tgccacaaca ccttccagcg gttgatcgat atccaccacc
    79501 agtacatcgg tttcgtccgc acccggctcc tgcagcgttt caaactgcgt caccaacatt
    79561 tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc tttcaatcac atcaaaatcg
    79621 cctttcaggt agatgaacga gagattcgga ttaccttcac gcagcaagtc gcgatagtgt
    79681 tttttcagtg cagaacagac gatcagcgac actttattgg tgcgctgcat agcaaacgcg
    79741 gcgtcgttca gcgcctgcaa ccacggtttg cgatcgtcgt cattcagcgg ttcaccagac
    79801 gccatttttt cgatattgcg ccttggatgg aggaaatcgc catcaagaaa cgcggcatga
    79861 agttgatgcg ccacttcact ggcgaccgca gatttgccgc tgcccgatac gcccatcaag
    79921 acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg tgcccccaca atacaagaaa
    79981 taaaaatgtt acgggtaact gttatcggta acattgtccg gccggacaat atcagaagca
    80041 atatccatcc gtgccttaag tgtataagtg tgagctactt caaatttgtg ggcttaaata
    80101 gatccgcccg gtgacaaggt gaaacctaaa tctaacattt tcggtgtcac agattcgcca
    80161 cgaatacgcg ccagcaggcg ttcagcgcca atactgccca tccgctcacg cggcgtcagt
    80221 acgctcgcca accgtggttc catcacctga ccaatgtcat gaccatggaa accggcaatc
    80281 gccatatcgt caggaacttt caaccccaga cgctggcatt caaacgccgc gccgaccgcc
    80341 aggtcatcat tagtacagaa cacgccatcc agctgcggat attcccgcct cgcctggcga
    80401 atcagttcaa taccggaaga gtaagaagaa gattgctcaa ccatcacgct atacggcacc
    80461 aggcctgcat ccagcatcgc ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg
    80521 agacgtgcgc cgagataggc aatgtggcga tgcccgcgag caataatggc ggtggtcatc
    80581 tggcgtgccg cttcaaagtt atcaaaaccg acggcaatat cgaggcacgg cgacttgctg
    80641 tccatcaact ccaccaccgg aatccccgcc acttcaatca tctttaaggt gcgtggcgtg
    80701 tgggtacgtt cggtgaggat cagtccatcg atattccatg agagcattga ttcgaggcgt
    80761 tcttgctcca tttccggttt ataaccgtag tgcgccagca tagtttgata accgtgcgcg
    80821 tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa cctggttggt gagagaaggt
    80881 aacaggacgc caatcgcccg gctggtggca ttagagagga tatcgggcgc gcgattggga
    80941 atataaccca gttcatcaag agcggcggca atcttgccgc gtagagcgac ggaaacctgc
    81001 tccgggttgc gtaaaaaacg gctgaccgtc attttggtca cgcctacacg gtcagccaca
    81061 tcctgaagta cgggtctttt ctttttcatc gtcctgaagg tacaaaagag atagattctc
    81121 ttagtttaac acggacgcat cagaaccttc ccggggaaaa cagtgcttgt ttacaattat
    81181 tagatataca ctaaatcaat tgcatcgcat tgtgctaacg acgcggcaaa accccggaag
    81241 cgtacatcag cacgcgaccg gagcgagtga ggaagcctgc aaaaaggcag gcttcaaaga
    81301 tgccgttgag ttttaaaccg gcggcagatc gaacagtaac acttcgctgt cgctatccgc
    81361 atgaatggag attgcctgct catcccagat tgccagacca tcgctggtcg aggctttcac
    81421 gccgttaatg gtgacattgc ctttcaccac ctgaatccag acgcggcgtt cagcggcaat
    81481 ctgatgcacc gactgctcat cttttaccag cgcccagcgg tacagttcca tatcctgata
    81541 cactttcagc gaaccatcac gcgcatccgg cgagagcacc agctgtttgc cctgtacggc
    81601 atcgaagcga cgctgttcat aacgcggcgt aataccgttt tcttcaggca tgatccagat
    81661 ttgatacaga tgcagacgct cggtgctgct tgggttgtac tctgagtgac gaatacccgt
    81721 acccgcactc ataatctgga actcacccgc cggaacctgc tctttattgc ccatgctgtc
    81781 ctgatgctca acagtacctt ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg
    81841 agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa
    81901 gcccataaag ttcggatcgt aatagttggc aaaagagaaa gtatgccagg agtccagcca
    81961 gccatgattt gcatgaccac gttcatttgc tttgcgtaag tagatcattt ttattcgccc
    82021 cctgaatgat ttcgatgggc ttagtgtgga cccgatccgc ctgggatgat agagggtgaa
    82081 aattgacccc tctgttcaaa aaaattgaac tattcgaggg gccatcaaac ttacttcgcg
    82141 agagtcactg tggagggaga ggcttgctca aatcctcgtt caaggatttc cagattggta
    82201 agaacttcag attccttgac gtaatttggc gcaccgttgg tgatggtttg atacaacgca
    82261 tcataaacgc gcccgtaatc gcccatctcc ggcttcatct cttctctgac cgtcacaccc
    82321 tcgtcattga catactccag cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc
    82381 ggcataatat tagccttgag gctggtttcc tgctggtcga taccgtattt aataaacgaa
    82441 cctttcttac cgtgaacgat aaatttcgga taatcgattt tcaccagatg gctggttttg
    82501 acgatggctt tcaggtcgcc ataaaacagc tgcgcttcaa aggtgtcgtc aggattggct
    82561 ttattacgca ggctgcggat gtcataagcg acgtgatccg ggcgaccgaa cagagaaata
    82621 atctggtcca tcgtatgcac accaagacca tagaacgcgc catcctgcgg cagcccaggt
    82681 ttggtttctg ccaccgggcg gtaatagtca aaatggcttt ccacttcgac aatctctccc
    82741 aacttgccac tttcaatcgc ttttttcgct gtcaggaagc aggaatcaaa acgacgattc
    82801 tgatacggcg tgacggtcag tcctttgctt ttcgccagcg caaacagctc cttcgcctgg
    82861 gcaagcgtcg gggtgaacgg tttttcgacc agcacatttt tcccggcttc cagcgcgcgc
    82921 ttcgcgtact cgaagtggct gtctgcatgg gtgcagacca caaccagctt aacatcggga
    82981 tcgtttagta cttcgtcgag atcgctggtg aaatggatat gagagtaaat gggggcctgt
    83041 tcttccggct tcgcatggcg gcgaaaaata tgcgcgacat gccagctatc cttgcggtta
    83101 agtacatacg gcagatggta acgggtggtg cttttgccga agccaataaa ggcgcagttg
    83161 atgaccatga tttcgtcctt tttaaggtga ttatttacac cttagcgcaa agcggacgtg
    83221 gttcctactg gagtgcgcat aacgaacaca agcacttccc gtggataaat tgaaaacgaa
    83281 agatcaaaaa aaaagccagc acccggctgg ctaagtaata ctggaagcaa tgtgagcaat
    83341 gtcgtgcttt caggttctcc gcgagggtct tcctgatcgc aagacaataa taatcattct
    83401 cattcgcact tgtccaacac tttttgcaaa aaaaatgcat ttgactcgca tttgaaagtc
    83461 aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtgata
    83521 cgccatgctg aaacgcgcga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg
    83581 tattgcaaca cgctacaggt gcctcatcct tccgatcata tgtggcagga gcgactcgcc
    83641 gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt gggccatctc
    83701 accattgacg tgcaacagcg cccacgccgc agtcatgttg cagattttgg tatttgtgtc
    83761 gactcccgtt ggaagaatcg cggcgtcgcc agcgccctga tgcgagagat gattgaaatg
    83821 tgcgacaact ggttgcgggt agatcgtatt gagctaacgg tgttcatcga taacgcgcca
    83881 gcaattaaag tctataaaaa atttggattt gagattgaag ggactggcaa gaagtacgca
    83941 ttacgtaatg gtgaatatgt cga
//
LOCUS       DARNVM010000002        77725 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.002, whole genome shotgun sequence.
ACCESSION   DARNVM010000002 DARNVM010000000
VERSION     DARNVM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.002"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..436
                     /gene="agaB"
                     /locus_tag="SI621_000072"
     CDS             <1..436
                     /gene="agaB"
                     /locus_tag="SI621_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HEI4062843.1"
                     /translation="LVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQKLMGITAETYG
                     FGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDVNVGNMHFSEGK
                     KQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            475..1278
                     /gene="agaC"
                     /locus_tag="SI621_000073"
     CDS             475..1278
                     /gene="agaC"
                     /locus_tag="SI621_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HEI4062844.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1268..2059
                     /gene="agaD"
                     /locus_tag="SI621_000074"
     CDS             1268..2059
                     /gene="agaD"
                     /locus_tag="SI621_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HEI4062845.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2060..2827
                     /locus_tag="SI621_000075"
     CDS             2060..2827
                     /locus_tag="SI621_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HEI4062846.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSHLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2877..3740)
                     /gene="rsmI"
                     /locus_tag="SI621_000076"
     CDS             complement(2877..3740)
                     /gene="rsmI"
                     /locus_tag="SI621_000076"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEI4062847.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3805..5841
                     /gene="lpoA"
                     /locus_tag="SI621_000077"
     CDS             3805..5841
                     /gene="lpoA"
                     /locus_tag="SI621_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HEI4062848.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPAATTAAVSAPANPSAELKIYDTSSQPLSQILNQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGIAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5799..6194
                     /locus_tag="SI621_000078"
     CDS             5799..6194
                     /locus_tag="SI621_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEI4062849.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6214..6804
                     /gene="diaA"
                     /locus_tag="SI621_000079"
     CDS             6214..6804
                     /gene="diaA"
                     /locus_tag="SI621_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HEI4062850.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6814..7389
                     /gene="yraP"
                     /locus_tag="SI621_000080"
     CDS             6814..7389
                     /gene="yraP"
                     /locus_tag="SI621_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HEI4062851.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7511..8551)
                     /locus_tag="SI621_000081"
     CDS             complement(7511..8551)
                     /locus_tag="SI621_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEI4062852.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPLTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVIAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGIAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLA"
     gene            complement(8624..9259)
                     /locus_tag="SI621_000082"
     CDS             complement(8624..9259)
                     /locus_tag="SI621_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEI4062853.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9387..9905
                     /gene="yhbO"
                     /locus_tag="SI621_000083"
     CDS             9387..9905
                     /gene="yhbO"
                     /locus_tag="SI621_000083"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HEI4062854.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9885..10328)
                     /locus_tag="SI621_000084"
     CDS             complement(9885..10328)
                     /locus_tag="SI621_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HEI4062855.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10379..10681
                     /gene="yhbQ"
                     /locus_tag="SI621_000085"
     CDS             10379..10681
                     /gene="yhbQ"
                     /locus_tag="SI621_000085"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HEI4062856.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10668..11171)
                     /locus_tag="SI621_000086"
     CDS             complement(10668..11171)
                     /locus_tag="SI621_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4062857.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11165..11689)
                     /locus_tag="SI621_000087"
     CDS             complement(11165..11689)
                     /locus_tag="SI621_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEI4062858.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11898..12893
                     /locus_tag="SI621_000088"
     CDS             11898..12893
                     /locus_tag="SI621_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEI4062859.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12902..13780
                     /locus_tag="SI621_000089"
     CDS             12902..13780
                     /locus_tag="SI621_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEI4062860.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13861..14868
                     /locus_tag="SI621_000090"
     CDS             13861..14868
                     /locus_tag="SI621_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEI4062861.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14986..16230)
                     /gene="mtr"
                     /locus_tag="SI621_000091"
     CDS             complement(14986..16230)
                     /gene="mtr"
                     /locus_tag="SI621_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEI4062862.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16384..18273)
                     /gene="deaD"
                     /locus_tag="SI621_000092"
     CDS             complement(16384..18273)
                     /gene="deaD"
                     /locus_tag="SI621_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HEI4062863.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18266..18346)
                     /gene="yrbN"
                     /locus_tag="SI621_000093"
     CDS             complement(18266..18346)
                     /gene="yrbN"
                     /locus_tag="SI621_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HEI4062864.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18453..19337)
                     /gene="nlpI"
                     /locus_tag="SI621_000094"
     CDS             complement(18453..19337)
                     /gene="nlpI"
                     /locus_tag="SI621_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HEI4062865.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19446..21581)
                     /gene="pnp"
                     /locus_tag="SI621_000095"
     CDS             complement(19446..21581)
                     /gene="pnp"
                     /locus_tag="SI621_000095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEI4062866.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21828..22097)
                     /gene="rpsO"
                     /locus_tag="SI621_000096"
     CDS             complement(21828..22097)
                     /gene="rpsO"
                     /locus_tag="SI621_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEI4062867.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22246..23190)
                     /gene="truB"
                     /locus_tag="SI621_000097"
     CDS             complement(22246..23190)
                     /gene="truB"
                     /locus_tag="SI621_000097"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEI4062868.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23190..23591)
                     /gene="rbfA"
                     /locus_tag="SI621_000098"
     CDS             complement(23190..23591)
                     /gene="rbfA"
                     /locus_tag="SI621_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEI4062869.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23755..26427)
                     /gene="infB"
                     /locus_tag="SI621_000099"
     CDS             complement(23755..26427)
                     /gene="infB"
                     /locus_tag="SI621_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEI4062870.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26452..27939)
                     /gene="nusA"
                     /locus_tag="SI621_000100"
     CDS             complement(26452..27939)
                     /gene="nusA"
                     /locus_tag="SI621_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEI4062871.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27967..28419)
                     /gene="rimP"
                     /locus_tag="SI621_000101"
     CDS             complement(27967..28419)
                     /gene="rimP"
                     /locus_tag="SI621_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEI4062872.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28626..28702)
                     /locus_tag="SI621_000102"
     tRNA            complement(28626..28702)
                     /locus_tag="SI621_000102"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28666..28668),aa:Met,seq:cat)
     gene            29050..30393
                     /gene="argG"
                     /locus_tag="SI621_000103"
     CDS             29050..30393
                     /gene="argG"
                     /locus_tag="SI621_000103"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEI4062873.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30401..32026)
                     /locus_tag="SI621_000104"
     CDS             complement(30401..32026)
                     /locus_tag="SI621_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEI4062874.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDFVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(32483..32569)
                     /locus_tag="SI621_000105"
     tRNA            complement(32483..32569)
                     /locus_tag="SI621_000105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32533..32535),aa:Leu,seq:gag)
     gene            complement(32584..32916)
                     /gene="secG"
                     /locus_tag="SI621_000106"
     CDS             complement(32584..32916)
                     /gene="secG"
                     /locus_tag="SI621_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEI4062875.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33144..34481)
                     /gene="glmM"
                     /locus_tag="SI621_000107"
     CDS             complement(33144..34481)
                     /gene="glmM"
                     /locus_tag="SI621_000107"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEI4062876.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34474..35322)
                     /gene="folP"
                     /locus_tag="SI621_000108"
     CDS             complement(34474..35322)
                     /gene="folP"
                     /locus_tag="SI621_000108"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEI4062877.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35412..37346)
                     /gene="ftsH"
                     /locus_tag="SI621_000109"
     CDS             complement(35412..37346)
                     /gene="ftsH"
                     /locus_tag="SI621_000109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEI4062878.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37446..38075)
                     /gene="rlmE"
                     /locus_tag="SI621_000110"
     CDS             complement(37446..38075)
                     /gene="rlmE"
                     /locus_tag="SI621_000110"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEI4062879.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38201..38494
                     /gene="yhbY"
                     /locus_tag="SI621_000111"
     CDS             38201..38494
                     /gene="yhbY"
                     /locus_tag="SI621_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEI4062880.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38650..39126)
                     /gene="greA"
                     /locus_tag="SI621_000112"
     CDS             complement(38650..39126)
                     /gene="greA"
                     /locus_tag="SI621_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEI4062881.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39374..40807
                     /gene="dacB"
                     /locus_tag="SI621_000113"
     CDS             39374..40807
                     /gene="dacB"
                     /locus_tag="SI621_000113"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HEI4062882.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVFIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40847..42019)
                     /gene="cgtA"
                     /locus_tag="SI621_000114"
     CDS             complement(40847..42019)
                     /gene="cgtA"
                     /locus_tag="SI621_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEI4062883.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42035..43000)
                     /locus_tag="SI621_000115"
     CDS             complement(42035..43000)
                     /locus_tag="SI621_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEI4062884.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKKLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43127..43384)
                     /gene="rpmA"
                     /locus_tag="SI621_000116"
     CDS             complement(43127..43384)
                     /gene="rpmA"
                     /locus_tag="SI621_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEI4062885.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43405..43716)
                     /gene="rplU"
                     /locus_tag="SI621_000117"
     CDS             complement(43405..43716)
                     /gene="rplU"
                     /locus_tag="SI621_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEI4062886.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43975..44946
                     /gene="ispB"
                     /locus_tag="SI621_000118"
     CDS             43975..44946
                     /gene="ispB"
                     /locus_tag="SI621_000118"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEI4062887.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45174..45452
                     /gene="sfsB"
                     /locus_tag="SI621_000119"
     CDS             45174..45452
                     /gene="sfsB"
                     /locus_tag="SI621_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HEI4062888.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45500..46759)
                     /gene="murA"
                     /locus_tag="SI621_000120"
     CDS             complement(45500..46759)
                     /gene="murA"
                     /locus_tag="SI621_000120"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEI4062889.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46814..47068)
                     /gene="ibaG"
                     /locus_tag="SI621_000121"
     CDS             complement(46814..47068)
                     /gene="ibaG"
                     /locus_tag="SI621_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HEI4062890.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47228..47521)
                     /gene="mlaB"
                     /locus_tag="SI621_000122"
     CDS             complement(47228..47521)
                     /gene="mlaB"
                     /locus_tag="SI621_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEI4062891.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47521..48156)
                     /gene="mlaC"
                     /locus_tag="SI621_000123"
     CDS             complement(47521..48156)
                     /gene="mlaC"
                     /locus_tag="SI621_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEI4062892.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48175..48726)
                     /gene="mlaD"
                     /locus_tag="SI621_000124"
     CDS             complement(48175..48726)
                     /gene="mlaD"
                     /locus_tag="SI621_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEI4062893.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48731..49513)
                     /gene="mlaE"
                     /locus_tag="SI621_000125"
     CDS             complement(48731..49513)
                     /gene="mlaE"
                     /locus_tag="SI621_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEI4062894.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49521..50330)
                     /gene="mlaF"
                     /locus_tag="SI621_000126"
     CDS             complement(49521..50330)
                     /gene="mlaF"
                     /locus_tag="SI621_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HEI4062895.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50540..51517
                     /locus_tag="SI621_000127"
     CDS             50540..51517
                     /locus_tag="SI621_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEI4062896.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPPDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51531..52517
                     /gene="kdsD"
                     /locus_tag="SI621_000128"
     CDS             51531..52517
                     /gene="kdsD"
                     /locus_tag="SI621_000128"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HEI4062897.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52538..53104
                     /gene="kdsC"
                     /locus_tag="SI621_000129"
     CDS             52538..53104
                     /gene="kdsC"
                     /locus_tag="SI621_000129"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HEI4062898.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLLPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53101..53676
                     /gene="lptC"
                     /locus_tag="SI621_000130"
     CDS             53101..53676
                     /gene="lptC"
                     /locus_tag="SI621_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEI4062899.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53645..54202
                     /gene="lptA"
                     /locus_tag="SI621_000131"
     CDS             53645..54202
                     /gene="lptA"
                     /locus_tag="SI621_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HEI4062900.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54209..54934
                     /gene="lptB"
                     /locus_tag="SI621_000132"
     CDS             54209..54934
                     /gene="lptB"
                     /locus_tag="SI621_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEI4062901.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54982..56415
                     /gene="rpoN"
                     /locus_tag="SI621_000133"
     CDS             54982..56415
                     /gene="rpoN"
                     /locus_tag="SI621_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEI4062902.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56438..56725
                     /gene="hpf"
                     /locus_tag="SI621_000134"
     CDS             56438..56725
                     /gene="hpf"
                     /locus_tag="SI621_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HEI4062903.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56843..57334
                     /gene="ptsN"
                     /locus_tag="SI621_000135"
     CDS             56843..57334
                     /gene="ptsN"
                     /locus_tag="SI621_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEI4062904.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57380..58234
                     /gene="rapZ"
                     /locus_tag="SI621_000136"
     CDS             57380..58234
                     /gene="rapZ"
                     /locus_tag="SI621_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEI4062905.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58231..58503
                     /gene="npr"
                     /locus_tag="SI621_000137"
     CDS             58231..58503
                     /gene="npr"
                     /locus_tag="SI621_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HEI4062906.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58716..59348
                     /gene="yrbL"
                     /locus_tag="SI621_000138"
     CDS             58716..59348
                     /gene="yrbL"
                     /locus_tag="SI621_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HEI4062907.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAAQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALTIALQKDLQPRESKTLTLTSREA"
     gene            complement(59345..60073)
                     /gene="mtgA"
                     /locus_tag="SI621_000139"
     CDS             complement(59345..60073)
                     /gene="mtgA"
                     /locus_tag="SI621_000139"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEI4062908.1"
                     /translation="MSKSRLTVFSFIRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVNMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(60070..60723)
                     /gene="elbB"
                     /locus_tag="SI621_000140"
     CDS             complement(60070..60723)
                     /gene="elbB"
                     /locus_tag="SI621_000140"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HEI4062909.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60953..63289)
                     /gene="arcB"
                     /locus_tag="SI621_000141"
     CDS             complement(60953..63289)
                     /gene="arcB"
                     /locus_tag="SI621_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HEI4062910.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63385..64314)
                     /locus_tag="SI621_000142"
     CDS             complement(63385..64314)
                     /locus_tag="SI621_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEI4062911.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64989..69449
                     /gene="gltB"
                     /locus_tag="SI621_000143"
     CDS             64989..69449
                     /gene="gltB"
                     /locus_tag="SI621_000143"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEI4062912.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69462..70880
                     /gene="gltD"
                     /locus_tag="SI621_000144"
     CDS             69462..70880
                     /gene="gltD"
                     /locus_tag="SI621_000144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HEI4062913.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            71064..72176
                     /locus_tag="SI621_000145"
     CDS             71064..72176
                     /locus_tag="SI621_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HEI4062914.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSTRNLWQFKNFYICFQRIEI
                     VQTLSAQFTPTILQTVSGESCHLAQLAKSFPLPWSTYVRLLSVKKADARSFYEKETLR
                     CGWSARQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLEFLELK
                     DEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLL
                     IVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAYYALAGLP
                     NTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(72236..72700)
                     /locus_tag="SI621_000146"
     CDS             complement(72236..72700)
                     /locus_tag="SI621_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEI4062915.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            complement(72697..73572)
                     /gene="nanK"
                     /locus_tag="SI621_000147"
     CDS             complement(72697..73572)
                     /gene="nanK"
                     /locus_tag="SI621_000147"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HEI4062916.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTHAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(73569..74258)
                     /locus_tag="SI621_000148"
     CDS             complement(73569..74258)
                     /locus_tag="SI621_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEI4062917.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(74306..75796)
                     /gene="nanT"
                     /locus_tag="SI621_000149"
     CDS             complement(74306..75796)
                     /gene="nanT"
                     /locus_tag="SI621_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HEI4062918.1"
                     /translation="MSTSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(75905..76798)
                     /gene="nanA"
                     /locus_tag="SI621_000150"
     CDS             complement(75905..76798)
                     /gene="nanA"
                     /locus_tag="SI621_000150"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEI4062919.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(76920..77711)
                     /gene="nanR"
                     /locus_tag="SI621_000151"
     CDS             complement(76920..77711)
                     /gene="nanR"
                     /locus_tag="SI621_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HEI4062920.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        18970 a        21125 c        19388 g        18242 t
ORIGIN      
        1 tctggttcac ggtcaggttg gcgtgacctg gacatccacc atcggtgcaa atctgctggt
       61 agtcgtggat gatgttgtcg ctaacgatga tattcaacag aaattaatgg gtattaccgc
      121 ggaaacctac ggctttggca ttcgtttctt tactatcgaa aaaaccatta acgtcatcgg
      181 caaagctgca ccgcatcaga agatcttcct gatttgccgt acgccacaaa cggtacgtaa
      241 attggtagaa ggtggtattg acctgaaaga tgtcaacgtc ggcaatatgc atttctcgga
      301 agggaaaaag caaatcagca gtaaagttta tgtcgatgac caggatctca cggacttacg
      361 ttttattaaa caacgtggcg tgaatgtttt cattcaggac gtccctggcg atcaaaaaga
      421 acaaatccct gactaaatct aaaatcgcct taatattggt ttgaggtaat aaaaatgcat
      481 gaaataaccc tacttcaggg attatccctg gcggcgttag ttttctttct ggggattgat
      541 ttttggctgg aagccttatt tttattccgc ccgataatcg tttgtaccct aactggcgct
      601 attctcggtg atattcagac tggcttaatt accggtggcc tgacagagtt ggctttcgcc
      661 ggattaaccc ctgcaggtgg tgttcagccg cccaacccga ttatggcggg tctgatgacc
      721 actgtcattg catggtctac gggcgttgat gccaaaacgg caattggtct tggcctgccg
      781 tttagtttgt taatgcagta cgtcattctg ttcttctatt ccgctttctc attatttatg
      841 accaaagccg ataaatgcgc aaaagaggcg gatacggcag cgttttcccg acttaactgg
      901 acaacgatgc tcatcgtcgc ttcagcgtat gcggtgattg ctttcctctg tacttacctg
      961 gcacaagggg cgatgcaggc gctggtgaaa gcgatgcccg cctggctgac ccacggcttt
     1021 gaagtggctg gcggtattct gcctgccgtt ggttttggct tgctgctgcg cgtgatgttc
     1081 aaagcgcaat atatccctta cctgatcgcc ggtttcctgt ttgtttgcta catccaggtc
     1141 agcaacctgt tgccggttgc cgtactgggc gcaggctttg cggtgtatga gtttttcaat
     1201 gcgaaatccc ggcagcaagc gcaaccgcag cccgttgcca gtaaaaatga agaagaggac
     1261 tacagcaatg ggatctgaaa tcagtaaaaa agatatcacc cgtctgggct ttcgttcgtc
     1321 actgctgcaa gcgagcttta attacgaaag gatgcaggcg ggcggtttta cctgggcgat
     1381 gttgccgatc ctgaaaaaga tttataagga tgacaaaccg ggcctaagcg cagcgatgaa
     1441 agataacctc gaatttatta atacccaccc gaatctggtc ggattcctga tggggttatt
     1501 aatttcgatg gaagaaaaag gagaaaaccg cgacaccatt aaaggcctca aagtggcact
     1561 gtttggccca atcgccggga ttggcgatgc gattttctgg ttcactttgt tgccgattat
     1621 ggcggggatt tgctcatcgt ttgccagtca gggaaacctg ctggggccga ttctattttt
     1681 cgccgtttac ctgcttatct ttttcctgcg cgtcggctgg acccacgtcg gttattcagt
     1741 cggcgtgaag gcgatcgata aagtgcgaga gaactcgcag atgattgccc gttcggcaac
     1801 catcctcggg atcacggtaa tcggcgggct gatcgcttcg tatgtgcata ttaacgtggt
     1861 gacatcgttt gccatcgaca gtacccacag cgtcgcactg cagcaggatt tcttcgataa
     1921 agtcttcccg aacattttac cgatggccta caccctgttg atgtattact tcctgcgggt
     1981 gaaaaaagcg catccggtgc tgttaatcgg cgtgactttt gtgctctcta ttgtttgttc
     2041 tgcattcggc attttgtaaa tggaacgagg cactgcgtct ggtggtgcct ctttactaaa
     2101 ggaatttcat cctgtgcaaa cccttcaaca agttgaaaac tatacggcgt taagtgaacg
     2161 tgccagcgaa tatttattgg ccgtgatccg tagcaaaccg gatgccgtga tttgcctggc
     2221 gaccggagcc acgccattac tgacgtatca ttatctggta gaaaaaatcc accagcagca
     2281 ggttgatgtc agccatctca ccttcgtgaa gctcgacgaa tgggtggatc tgccattaac
     2341 tatgcctggc acctgcgaaa ctttcctaca acagcatatc gtgcagccgc tggggctacg
     2401 tgaagaccag ctcatcagct ttcgctccga agagataaat gagacagagt gcgaacgggt
     2461 aacgaacctg attgcgcgca aaggcggcct ggatttatgc gttctcggat tggggaaaaa
     2521 cggtcatctt gggctgaacg aaccgggaga aaacctgcaa ccggcctgcc atatcagtca
     2581 acttgatgcc agaacacagc aacatgagat gttaaaaacc gcgggccgtc ccgtgactcg
     2641 tgggatcacc ttaggcctga aggatattct caatgcccgc gaagttttgt tactggtgac
     2701 tggcgaagga aagcaggatg cgacagatcg ttttctcacg gctaaagtct ctaccgctat
     2761 cccggcttca tttttatggc tgcataacta tttcacatgc atgattgatg agatgtgtcg
     2821 tcgttaactc gcctgctaca ggcgttgcaa cgctattcct gctgcgacgc ctgtaatcac
     2881 tctccctgct gctccagtgc atacttatac agcgcatttt tcttcacgcc gtgaatttct
     2941 gctgccagcg ccgccgcttt tttcagtggc agttctgcct gtagcagcgc cagcgtgcgc
     3001 agggcatcgg cgggtaagtc ttcttcctgt gctttatggc cttcgacaat cagtaccatt
     3061 tcgcctttgc gacggttttc atcttctttg acccacgcca gcagctcgcc aacgggcgcg
     3121 ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca gaacgacgta gcgggattcg
     3181 cctaataccg caacgatatc ttccaggcta tctaacagac ggtgggtaga ttcataaaaa
     3241 atcagcgtgc gcggctccgc ttcaatggct tttagcgcat cacggcggcc ttttgatttg
     3301 gcaggtaaaa agccttcata acaaaaacga tcggatggta aacccgcggc gcttaacgca
     3361 gtgatagcag cacacggccc gggtagcggc accacgcgaa tccccgcttc acggcaggta
     3421 cgcaccagat ggtagccagg atcgttaatt agcggcgttc cggcatcgga aaccagcgca
     3481 atgttttgcc cctcttgcag cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg
     3541 tcgtgcagcg caaacaaccg agcattaatc ccaaaatgtt gcagcaataa accggtgtga
     3601 cgcgtatcct cggcggcaat cagatcaacg gcctgtaata cctccaacgc acgctgggtg
     3661 atatccgcca gattgccgat tggcgtcggt acaatgtaaa gctggccctg agaattatcc
     3721 gccgattggt gttgtttcat tgtgtcgtcc gtattgccga tttaatattg agcattgcgt
     3781 aaaaaaaata tcactggata cattatggta ccctcaacat tttctcgttt gaaagccgcg
     3841 cgttgtctgc ctgttgttct ggcagccctg attttcgccg gttgtggcac ccatactccc
     3901 gatcagtcca ctgcttatat gcagggcacg gcgcaggctg attctgcctt ttacctgcaa
     3961 cagatgcagc aaagttctga tgataccagg atcaactggc aattactcgc cattcgtgca
     4021 ctggtgaaag aaggtaaaac cggacaggcg gtggagttgt ttaaccaact accgcaagaa
     4081 ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg cagagattaa actggcgcag
     4141 aaagattttg ctggcgcgca aaacttgctg gcgaaaatca cacctgcaga tttagaacaa
     4201 aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca gccaggggcg tccttccatt
     4261 gatttactgc gcgcgttaat tgctcaggaa ccgctgctcg gcgcgaaaga aaagcagcag
     4321 aatattgacg ccacctggca ggcgctctcc tccatgactc aggaacaggc gaatacgctg
     4381 gtgatcaatg ccgacgaaaa tattctgcaa ggctggttgg atctgcagcg catctggttt
     4441 gataaccgta acgatcccga catgatgaaa gccgggatcg ccgactggca gaaacgttat
     4501 ccgaacaacc cgggcgcgaa aatgctgcca acgcagttgg ttaacgtaaa agcgtttaaa
     4561 cccgcatcga ccaacaaaat cgccctgctg ttgccactga atggccaggc agcggtattt
     4621 ggtcgcacta ttcagcaagg ctttgaagcg gcgaaaaata tcggcactca gccagtggcg
     4681 gctcaggtag ctgccgcacc tgccgcagac gtagcagaac aacctcagcc gcaaaccgtg
     4741 gatggcgttg ccagcccggc acaagcctcg gttagcgatc tgaccggtga acaacctgcg
     4801 gcccagccgg tgcctgtaag cgccccggcg gcaaccaccg cagcggtaag cgcacccgcg
     4861 aatccatccg cagagctgaa aatctacgat acatcatcac aaccacttag ccagatcctc
     4921 aaccaggttc aacaggatgg cgcgagtatt gtggtcggtc cgttgctgaa aaataacgtt
     4981 gaagagttgc tgaagagcaa caccccgctg aacgtgctgg cactgaacca gccggagaat
     5041 atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac cggaagacga agcgcgcgat
     5101 gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc tggtgctgat cccacgcagt
     5161 tcattgggcg atcgcgtagc caatgcgttt gcgcaagagt ggcagaaact gggtggcggc
     5221 accgttctgc aacaaaaatt tggttccacc agcgaattac gcgcgggtgt taacggcggt
     5281 tctggtattg ctttaacggg tagcccgatt actcccagag cgacaaccga ctccggcatg
     5341 acgaccaaca atccaacgct gcaaaccacg ccaaccgatg accagttcac caataatggc
     5401 ggtcgtgtcg atgcggtgta cattgtggca acgccgggtg aaatcgcttt tatcaaaccg
     5461 atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc tgtacgccag ttcccgcagc
     5521 gcacaaggca tcgcaggccc ggatttccgt ctggagatgg aaggcttgca gtacagcgaa
     5581 atcccgatgc tggcaggcgg taatctgccg ttaatgcagc aggcgctcag tgcggtgaac
     5641 aacgattatt cgctggctcg catgtatgcg atgggcgtcg atgcctggtc gttggcaaat
     5701 catttctcac aaatgcgcca ggttcagggt tttgaaatca acggtaatac cggaagtctg
     5761 acggctaacc cggattgcgt gattaacagg aagttatcat ggctacagta ccaacaaggt
     5821 caggtagtcc ccgccagtta accaccaaac agaccggcga tgcgtgggaa gcacaagcgc
     5881 gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc taacgtgaac gagcgtggcg
     5941 gcgagatcga tctgataatg cgtgaaggcc ggatcaccgt ttttgtcgag gtgcgttacc
     6001 gccgctctgc gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa caacacaaat
     6061 tattacagac tgcccgcttg tggctcgcgc gtcataatgg gagttttgat actgtggatt
     6121 gccggttcga tgtggtagcc ttcaccggga atgaggttga gtggattaag gatgccttta
     6181 atgaccactc ataattaagg tttaaggatt agcgtgcaag aaagaattaa agcttgcttc
     6241 actgaaagca ttcaaactca aattgcggcg gcagaggcgc ttccggatgc catctcccgt
     6301 gcagccatga cgctggttca gtctctgctc aatggcaaca aaatcctctg ttgtggtaat
     6361 ggaacttccg ctgccaatgc acagcatttt gctgccagca tgatcaaccg tttcgaaacg
     6421 gagcggccca gcttacctgc cattgcacta aatactgata atgttgtctt aacggcgatt
     6481 gccaacgatc gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct gggtcatgcg
     6541 ggagatgtat tgttagccat ttccacccgt ggcaacagcc gcgatattgt aaaagcggta
     6601 gaagctgccg ttacgcgtga tatgaccatt gtggcattaa ccggctatga cggcggtgaa
     6661 cttgcaggtt tgttagggcc acaggatgtg gagatccgca ttccttcgca tcgtagtgct
     6721 cgcattcagg aaatgcatat gctgacggta aattgcctgt gcgatctgat cgataacacg
     6781 cttttccctc accaggatgt ttaaggagaa tacatgaagg cattatcgcc aatcgcagtc
     6841 cttctttccg cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg taccgctgct
     6901 gtaggtacca aagccgcaac tgacccacgc agtgtcggca cccaggtgga cgatggtacc
     6961 ctggaagtgc gcgtaaacag cgcattgtcg aaagacgaac agattaagaa agaaacccgt
     7021 attaatgtca cggcgtatca gggcaaagtg ctgctggttg ggcagtcacc aaacgctgaa
     7081 ctttcggctc gcgctaaaca aattgcgatg ggcgtagacg gtgccaacga agtgtataac
     7141 gagattcgtc agggccagcc gattggtctg ggcgaagcgt ctaacgatac gtggatcacc
     7201 accaaagtgc gttcgcagct cttaaccagc gacctggtga aatcgtccaa cgtgaaagtg
     7261 accaccgaaa acggtgaagt gttcctgatg gggctagtga ctgaacgtga agcgaaagcg
     7321 gcggcagata ttgccagccg ggtaagcggc gtgaagcggg taaccacggc gtttacgttt
     7381 attaaataag ccattgatcg gtcgttgaag taaatattca caccttagcc gacctgttcc
     7441 cctcacccta accctctccc caaaggggag aggggaccgt ccagtgctgt attcaggagc
     7501 atccaacatc ttaggccaac agcgccagcc cgccgacaat cacacctgac actgccacca
     7561 tcgcccccgt cagccataag gctttcgccg ggaacgcttt acgcaacata atcagtgacg
     7621 gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcgatt cccatgcctg
     7681 ccagcatcat cgtttgtaca attggaattt ctgctgccgt tggaatgaca aacaagcatc
     7741 ccgctaccgc catcgcaatc acccacatca ggctgttatc gacagcacca tcagcatggg
     7801 ggaataacca gacgcgagcg gcacccaaca cgagtacggc aaggatataa accgggatcg
     7861 tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaatccg ccctgcgctt
     7921 ccggtatgtc aatttcgacc ggtgcctgcg ttagcggtgt ttcacgtacc catttttgca
     7981 ccagcgtcgc aatcagcaac accatcacca gccccgcgac cagacgaatc gccgcaaacc
     8041 cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacaac ggattcccca
     8101 tccagaatgc cagcgccccg cccatcgata cctgttggcg acgcattccc gccgcgaccg
     8161 gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc
     8221 cgcgaaagcg cgattgccca agcgtacgca gcaaccagtc acgcgggatc agcacctgaa
     8281 tcaacgaccc gagcagtacg cccagcaccg ccgctttcca taccgcgagg aaatagatca
     8341 tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac
     8401 cgatactgtg ggtttcggca gccgtaaacg ctttgccgta gtaaggttgc catttcacat
     8461 accagagacc agcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg
     8521 ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat
     8581 tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc
     8641 gtaattctga agagcttaaa atcgtcacac cttcatgctc aggccgcatc gcctctgcca
     8701 gcatgacccg cgccacgtct cttgcatcaa tggatttcca gttacccggt aacaaacgga
     8761 acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agcaacatcg
     8821 aagggcgagc aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc
     8881 ccttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca ctgactacca
     8941 gcatatgctg cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat
     9001 cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa
     9061 acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc ggtcagttgc ggatcatggg
     9121 gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa
     9181 ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg
     9241 taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccccggc
     9301 tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtggga aattcgcctc
     9361 atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg
     9421 aatttgagga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag
     9481 tgattaccat tgaaaagcaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagtg
     9541 tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac
     9601 caggcggtca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcact ttcacccgtg
     9661 attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ttgctgatca
     9721 gcgccgatgt gattcggggg cgcaaactga ccgcagttaa accgatcatt attgatgtta
     9781 aaaacgcggg cgcggaattt tacgatcagg aagtcgtcgt cgataaagat cagctggtta
     9841 ccagccgcac accggacgat ctgccagcgt ttaaccgcga ggcattacgc ctgctcggtg
     9901 cctgagttac gtagccagat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg
     9961 atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcgt
    10021 cgattgtacg cctggcgtgc gaggtcgctt tcctcacctt ccagtctgcg gatctcacct
    10081 ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccagca
    10141 actgccgcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag
    10201 aaggcaactt tctgcgcatc aaaaagataa aaggcattgg cgcaccacaa ttccccttcc
    10261 tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat ggtgatgagt
    10321 gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat
    10381 gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata ccgggatcac
    10441 cacagatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg
    10501 cgggaaagga gaactaacgc tggcgttttc cgcgccagtg ggcgatcgtt cgctggcact
    10561 acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga
    10621 aggcgcaggg tttgcagaac tgttgagcag cctgcaaacc ccaaagatta aaagcgattg
    10681 aagtgctcgt gatactcaac cagaccggtt acgccgttca gcgcgtcatc cgctaaacga
    10741 tgtacctgga aggcgctttc agtgcccggc cagcggcaac gcagatcgtg atgcgccgcc
    10801 agctcaaagc cgaaacgact gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag
    10861 ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca
    10921 cggtattttt catcaaccgc caacggtgcc atgccgaccc actgcaggtc ttcgccctgc
    10981 acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc
    11041 agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca
    11101 ctttcgaatg aacgacgcag cagggcatca atacccggcg catcaatggg aatttctact
    11161 cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg
    11221 cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca
    11281 gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca
    11341 ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca
    11401 gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggcta acgaccagtt
    11461 tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc
    11521 agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat tgccagctta
    11581 agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca
    11641 acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc
    11701 cctgtttcaa caatcatcct attttgccat attagaaaaa taacatagcg gtataaatca
    11761 acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa
    11821 ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc
    11881 aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg
    11941 cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat actaatgccc
    12001 gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc
    12061 atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcacccg gacggttacg
    12121 cccgttggca gcgcgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg
    12181 ccgacctcgc catgctggag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt
    12241 cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg
    12301 ttgcacgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa ctggcgcggg
    12361 tcacgcctgt accactggaa gtctttgctt tcggcagcct gtgcattatg tcggaaggcc
    12421 gttgctatct gtcgtcatat ctgacgggtg aatcgcccaa caccgtcggt gcatgttctc
    12481 cggctcgctt cgtgcgctgg cagcaaacgc cgcaggggct ggaatcccgc ctgaacgaag
    12541 tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgctg tgtaaagggc
    12601 gttatctggt ggacggcgag cgctatcacg cgctggaaga acccaccagt ctcaataccc
    12661 tggaactgct gccggagtta atggcagcga atattgcttc ggtgaaaatt gaaggccgcc
    12721 agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt
    12781 gtaaggccga tccgcaaaac ttcgtaccgc aaagcgcgtg gatggagacg ctcgggtcga
    12841 tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaaaagc
    12901 aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc tggaagaatt
    12961 ttatcagcag gccgccgcca gcagcgccga cgtgatttat cttggtgaag cggtatgcag
    13021 caagcgtcgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcac tcgccggaag
    13081 tggtaagcag attgtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga
    13141 actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt
    13201 gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa
    13261 cgcggtgaca ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga
    13321 gctttcccgc gactggttgg tgaatctgct taatcagtgc gatgagctgg gcattcgcaa
    13381 ccagtttgaa gtggaagtcc tgagctacgg tcatctgccg ctggcctact ccgcccgctg
    13441 ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta
    13501 tccgaacggg cgcaacgtgc tatcgcagga aaaccaacaa gtgtttgtac tcaatggcat
    13561 tcagaccatg agcggctacg tttacaacct cggtaacgag ctgacatcca tgcaggggct
    13621 ggtcgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcaatgc tcgacgcctt
    13681 ccgcgccaat gaaaatggcg ctgcaccact gccgttgacg gcaaacagcg attgtaacgg
    13741 ctactggcgg cggctggccg ggctggagct gcaagcgtaa gtatatagct cactttgtta
    13801 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    13861 atgactgata aaaccattcc gttttcgcta ctcgatctgg cccccatccc cgaaggttct
    13921 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    13981 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    14041 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    14101 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaatacg
    14161 ctctatccgg ggcgaatcga tttggggctg ggtcgtgctc cgggtagtga ccagcgaacc
    14221 atgatggctc tacgtcgtca tatgagcggg gatattgata atttcccccg cgatgtcgcg
    14281 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    14341 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    14401 gcgcagcttg gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat gttgttccag
    14461 gcgctgcatc tttatcgcag taacttcaaa ccgtcggctc ggctggaaaa accatacgcg
    14521 atggtgtgca tcaatattat cgctgccgac agcaaccgcg acgctgaatt tctgtttacc
    14581 tcaatgcagc aagcctttgt gaaactacgc cgtggcgaaa ccggacaact accgccaccg
    14641 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    14701 agtatgtcgt tggtaggcga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc
    14761 gaaaccgacg ccgatgagat tatggttaac gggcagattt tcgatcacca ggcacgcctg
    14821 cattcgtttg aactggcgat ggatgttaag gaagagttgt tgggatagtg tgtcttaacg
    14881 cgggaagcct tatccgagat ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    14941 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    15001 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa ataccagaat
    15061 cagcataatc atcggcttgc cgccccagac gcggaatttc gggctaccaa agcgtttacg
    15121 cgatgcgcgg gccaacagcg ccggaacaat tgccgcccag atggtagccg ctaagccagc
    15181 ataaccaatg gcgtacagga atccgttagg aaacagcagg ccacccacaa caggcggggc
    15241 aaaggtcagc aacgctgttt tcaaacggcc catagccgag tcgtcgaaac caaacagatc
    15301 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    15361 tgagaacacg accagcagaa gatccaggct acggctgttc agcacgccgc ttaatgcctg
    15421 taccagcaca tcaatattac cacccttctc ggcaatgcca ataaactcag gacgagggat
    15481 gttgcccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    15541 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggcttggcac
    15601 gttaccgtga taaccaaacg acgccagaca gaacggcagg gtcatcaaca gatacggtgc
    15661 ataagacgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    15721 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agcacaatcg ccgtcatgcg
    15781 gctgacggct ttagtgctca accacaccac aaacgccacc agcagtgcaa aaccaaaacc
    15841 cgctgcccgt gccgggacgt tcaatgacat ctctgcgaag gtatgatgca gaatcgaacc
    15901 gctggcagaa atataggcat aggtcaggat ataaagcaca aaggcaatgg aaatgccgtt
    15961 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaagc tcgaaccgat
    16021 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    16081 aaagatcagc gccgccattg accagaagaa ccacgccccg gacatgacca caggcagaga
    16141 aaacatccct gcgccaataa tggtgccgcc gatgatcacc acgccgccaa gcagcgacgg
    16201 tgacgtttgg gtggtggtta gtgttgccat gagggcttct ctccagtgaa aaatagtgcg
    16261 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    16321 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgtttagcga
    16381 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    16441 gccttcacga cgttcgccgc taaaacgacg accatcacca cgaccacctt cacggcgttc
    16501 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    16561 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    16621 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    16681 gatggtggag tgagaagcaa acagcttgat gttaccgatg taacggctgc tgatgtcgcc
    16741 ttcgttagca atcgcgccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    16801 aatgcggtac agctgcatgt caccaacatc acgacgttca cgacgcggac gatcttcacg
    16861 gtcgccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    16921 ttcacgtttc ggacgcatcg gcgcatctgg cggaacaatc agagtacgtt caccctgtgc
    16981 cattttcagc agtgcggcgg ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    17041 ctgaattttg ctcagcagtg cacggtattg atccagatcg ctgctttcca gctgctgctg
    17101 tactttagcg gcgaattttt ccagacgacg tttgcccagc agttctgcgt tcggcagttc
    17161 aacttccgga atggtcagct tcatggtgcg ttcaatgtta cgcagcagac gacgctcacg
    17221 gttctcaacg aacagcagcg cacggccagc acggcccgca cgaccagtac gaccgatacg
    17281 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    17341 aacgtccagg ccacgggccg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    17401 cagtcgttcc agtgtctgct cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    17461 attgtagccg ttacgctcaa gagcttctgc cacttccaga gtcgcgtttt tggtacgaac
    17521 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    17581 acgcataccc caaacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    17641 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    17701 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatagt
    17761 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    17821 gctcagttta gaaaggtcca gagtgccacg tttcaggtga tccagcagac ggcccggagt
    17881 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    17941 gccgtacagg gcaaccacgt ttacgccgcg catgtgttta gagaaatccg tcattgcctc
    18001 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    18061 ctcaggatca agattctgca gcagaggtaa agagaatgct gcagtttttc cgctccccgt
    18121 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    18181 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    18241 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    18301 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    18361 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    18421 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    18481 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    18541 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    18601 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    18661 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    18721 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    18781 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    18841 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    18901 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    18961 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    19021 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    19081 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    19141 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    19201 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    19261 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    19321 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    19381 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    19441 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    19501 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc cagaactttc
    19561 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    19621 atctgagaga tgtgtaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    19681 tcaacgatac gggtcacttt accattgtag acgcggccca cttcgatttc tgcagtgatc
    19741 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    19801 gtaccgtcat cttcgatttc gatggtggtg ccagtttctt cggtcagggc acggataaca
    19861 gagccgcctt taccgataac gtctttgatc ttgtccgggt tgatcttgat ggtatggata
    19921 cgcggagcga actcagagat atcgccacgt ggcgcgttga tcgcctgttc cattacgccc
    19981 aggatatgca gacgcgcacc tttagcctgg ttcagtgcaa cctgcatgat ctctttggtg
    20041 ataccttcaa ttttgatatc catctgcaat gcagaaatac cgtcgcggga acccgcaact
    20101 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    20161 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cagcagcttt gatcggcaca
    20221 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    20281 gattcggtga tttcagacac cacacgtacg gtgtacggga atttgtccat atccggcatg
    20341 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    20401 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggtg
    20461 tcagtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgtgcagt acccagcgtt
    20521 gcggtaacca gcgcctgagt ttcaccacgg gtaaacagcg cagaaccgtg agtacgcggc
    20581 agcacgccag tacgcacatc cagaccacga atcatatctt tttcacgacc gtcgatacgc
    20641 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    20701 cccagttcgt tttcgtccag ggtttcatct tcagcaagca gcgtcgcgat ggtttcagat
    20761 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcgctc
    20821 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    20881 tgccagtccc aacgcggttt gccggcttct ttcaccagtt cattgatgtt ctgaataaca
    20941 acctgctgtt gttcatgacc gaacactact gcgcccagca tctggtcttc gctcagcagt
    21001 tcagcttcag attcaaccat cagtacagcg gcttcagtac ccgcaacaac cagatccagt
    21061 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    21121 acgcgggcag caccaatcgg accattgaac ggaataccag acagagacag tgctgcggaa
    21181 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    21241 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    21301 agacgcgcga tcagggtttc gccttcgctt gggcggcctt cacgacggaa gaagctaccc
    21361 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    21421 tgacctggtt tggctttttt ctggccaaca acggtaacaa ataccgcggt gtcatccatg
    21481 ctaaccataa cagcagcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    21541 tgttgaccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    21601 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    21661 aatgacaacc ctaacccagc ccaatgtggg taaagcctct cattagccgc gcgaacctct
    21721 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    21781 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    21841 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    21901 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    21961 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    22021 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    22081 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    22141 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    22201 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    22261 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    22321 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    22381 aaccgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    22441 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cggctggaat
    22501 atcctgctgt tcggcttgtt caaccagttc acgcagatgt tccagggtca ccatccgttc
    22561 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    22621 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    22681 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    22741 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt tgccctggta
    22801 tttcagtgct gaatacatcg atgggatctg ttcgatatcg ccacggaaag tatccagtgc
    22861 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccatcggc
    22921 gtcagacgta tcggtacgct gtccaagacg cgcaatcacc cgatagcgtt tgtcggagtc
    22981 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa atcggcaaca tgccggtcgc
    23041 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    23101 ttgcagcgca tcgttgctgg acataccctg aggtttatcc agcaacaaaa cgccgttaat
    23161 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    23221 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatacg catcccttca
    23281 accagcgagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    23341 cccagcaggc tgcggatgaa accagaagct tcctgcaacg ctttgatgcc cgctttaacc
    23401 gcgtcttcat ctttgtcgtt gaggaacgtt acatatactt tggcatacgc caggtcgcga
    23461 gacatttcga caccggaaac ggtggtcatc atgcccagac gaggatcttt aatttcacgc
    23521 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    23581 tctttcgcca taataaattc tcctgacaaa aaaggggcca tagccccttt ttaaaaatta
    23641 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgcgagcgt catatcaggc
    23701 cacggctgcc ggatgcggcg taaaagcctt atccggccta caacccagac aaccttaagc
    23761 gatggtacgt tggatttcga tgatctcgaa tacttcgatc acatcgccag tgcggacatc
    23821 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    23881 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cgcgcagaac
    23941 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    24001 aaatttcggt gatttgaaca cgtcacgaac ttccgccaga ccgataatct gttgtttcag
    24061 ttccggagac agcataccgc tcattgccgc tttcacttcg tcgatcaggt tatagatgac
    24121 ggagtagtaa cgcagatcca ggctttccgc ttcaattact ttacgtgcag aggcatcagc
    24181 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtag cgtcggtttc
    24241 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    24301 cagcaaggag tcggagatag cttccacaga accctgtacg tctgccttca ggacgatatt
    24361 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    24421 ctgacgcgcc agtttaactt cgcggaattt accctgacga tagagtgcaa cttcacgcgc
    24481 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggaacgc cggacaggcc
    24541 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    24601 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    24661 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    24721 aaccgcaccg ctcgccatgc ctttacgtac cgctttcagt tccagaactt ccgcctgcag
    24781 caggatagcg tccagcagct catcgatacc agtacccgct ttcgcagaca catgtacgaa
    24841 ctggctttca ccgccccact cttccggcag gatgccgtac tgggagagtt cgttcttaac
    24901 gcgatccgga tcggcttctg gtttatcgat cttgttcact gcaacgacca ccggaacctg
    24961 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc attacgccgt cgtcggcagc
    25021 aacaaccagg actacgatgt ccgttgcctg cgcaccacga gcacgcattg aagtaaacgc
    25081 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    25141 accaatgtgc tgggtaatgc cgcccgcttc gccagaggcc actttcgttg aacgaatgta
    25201 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    25261 cggttcagcc gctgcacccg tgtcacggtc gctcattacc gcttcttcca gctcgttttc
    25321 acgacgcagg ataactttgt ggcccatctc ttcggcaacc agctgtgcgg tttcctggtc
    25381 gatgacctgg ttgatggtcg ccattgcgcc cagcttcatc atcgctttga tgacctgaga
    25441 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    25501 gtcacggtta acggcctgag caggcttctg gaagccttgc tgcagcgaag aacctttacg
    25561 ttttccgccc ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    25621 agcgtgtttg ttgcctttct tcggacgcgc agctttcgcg ttacgaccac ggccacggcc
    25681 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    25741 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    25801 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gacgcgcttc
    25861 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    25921 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    25981 tttcgcttgt tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt cagctttttg
    26041 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    26101 ctgcgcttgc tcttccgcag caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    26161 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    26221 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    26281 caggtggtca atcaaagtct gtttctcttg tgcggacaca gagtcgtcag cagacttccg
    26341 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    26401 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    26461 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt
    26521 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    26581 aacgccacgg gcggccagtt tgaatgccaa atcacgatct atcccttcaa ggttcagcag
    26641 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    26701 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    26761 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    26821 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    26881 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    26941 accgctcagc tgcgaagcca gacgcacgtt ctgaccatta cggccaatcg cctgcgccag
    27001 gttaccggct tcaacggcga tatccatggt gtgcttatct tcatccacca cgatagaagc
    27061 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    27121 gatatcgata cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat
    27181 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    27241 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttccgg
    27301 cacttcaatg cggaacagtt cgatcagcat ttccggcttg gaacgggtga cgaacagttg
    27361 cgcgccacgc gcttccgggc gaacggaata gagcacgcca cgaacgcggt cgccaggacg
    27421 gaagttttca cgcggcagca tatcttcgcg caggatcacg gcttcagcgt tgttgcccag
    27481 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    27541 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    27601 aacctgtttt gccgtctggg tggtgatacg gtcaaaggta acagactcaa tctgatcttc
    27661 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    27721 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    27781 gcttttgcga tcgatctgta cgcgaacgtc gatttcttgt tcatattttt tctttgttgc
    27841 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    27901 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    27961 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    28021 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    28081 ccatttacga cggttttgta ccgccatgcg gagaaccaga gtcacctctt ctccgacgaa
    28141 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    28201 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    28261 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    28321 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    28381 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    28441 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    28501 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    28561 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    28621 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    28681 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    28741 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    28801 gtgatccatg ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccggttc
    28861 actttcattt gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca
    28921 aaaaacacac taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat
    28981 tttgcataaa aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg
    29041 tgttatttta tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct
    29101 ttttccggcg gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt
    29161 ccttatgcat atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct
    29221 cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg
    29281 gtggccgaag gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg
    29341 acctatttca acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcc
    29401 atgaaagaag atggcgtgaa tatctggggt gacggcagca cctataaagg aaacgatatc
    29461 gaacgtttct atcgttacgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg
    29521 cttgatactg actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt
    29581 gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct actccacgga ctccaacatg
    29641 cttggtgcaa cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc
    29701 aatccaatta tgggcgtgaa gttttgggat gagagcgtga aaatcccggc agaagaagtc
    29761 acagtacgct ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac
    29821 gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac
    29881 cagattgaaa accgtatcat cgaagcaaaa agccgcggca tttatgaagc ccctgggatg
    29941 gcactgctgc acattgcgta tgaacgtttg ttgaccggta ttcacaacga agacaccatt
    30001 gagcagtatc acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg acgttggttt
    30061 gactcccagg cgctgatgct gcgggactct ctgcaacgct gggttgccag ccagatcact
    30121 ggtgaagtta ctctggagct gcgccgcggg aacgattatt caatcctgaa taccgtctca
    30181 gagaacctga cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg
    30241 ccagatgatc gtatcggtca attgaccatg cgtaacctgg atatcaccga tacccgcgag
    30301 aaacttttcg gttatgccaa aactggcctc ctctcctcct ctgccgcttc gggcgtgccg
    30361 caggtggaga atctggaaaa taaaggccag taattcgaca tcaaccctgc ccttcagggg
    30421 tggggttacc ttgcttatcc tcagtaaact cctttcttag catcacaaaa tcgaaaacat
    30481 catggtttgc atctacaacc cgtgagtctc ctttatagtg tgcaatcact tcctccagcg
    30541 tttgcggctg ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtacg
    30601 cagtggaaaa gatatcgttt tccgttgtgc gatccacgcc atccccaaga acggggctat
    30661 accagataaa catcgggaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat
    30721 agacgttctt cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg
    30781 cgcggcgatc ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg
    30841 agttgtcgta gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat
    30901 aggcgctaca agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct
    30961 gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa caattcatca aatcctctga
    31021 catagactgt ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa
    31081 aagcggattg cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa
    31141 tgttgtcggg gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg
    31201 gaaccgacag cgcagtgtaa ggtgcaccgc ttattgcttg attaaacagt ttgatctgtt
    31261 ttctttgtgc ttcgacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt
    31321 cgacacgtac agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg
    31381 ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg
    31441 ctaaagcaaa ataattgaga ttgaaaaacg gtgtataggt agcaaatcgc gacgctagta
    31501 tatatggact gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa
    31561 ataaactgcc cgaaatgaca atcagcaata atattcctgt cacttttttc gtcggcaagg
    31621 aaacatcata ttttattatt actgcaaaaa aaagaaggga taaaaaggca aaagcacata
    31681 gataagggat atacatcccc agcattttga caacttcatc cggatcactc tgcagcacgc
    31741 taatcgcaaa tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac
    31801 tcatgtctgc cgctgtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg
    31861 atgatatacg tttaacaacc aggatggtca gaacgaaaaa cagcaatcgc tgaaccatat
    31921 gcgctccgga agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca
    31981 accaatgaga tttaaaactt ctaacaaatt tgttgaatac tgtcatccag aaatccaagt
    32041 tcacaaccaa tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa
    32101 ctctgatcca tatcagaccg aaacactgtt agttttttct ttttaaatga tgcggccatt
    32161 gttctcagta attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca
    32221 tcagtagtta tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg
    32281 taccggaact ggagttgaca caataaagtg ccaattatgt cagcagaagg gaaaagttta
    32341 taactaaggc gtaccggcac catcgtttca aagtaccagc tacgagtaaa gcaactggac
    32401 gagatacaga tacctgacaa ccattcctca gaccaggacc aaaacgaaaa aagacgcttt
    32461 tcagcgtctc ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt
    32521 gggcactacc acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac
    32581 tttttagttc gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt
    32641 tttggccggc gcactcaggt tttcccattc gctaccttta ttggttttgt tactattgat
    32701 gttacccagc accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt
    32761 catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga
    32821 ggctcccata tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc
    32881 cacaataagg aaaactacca aaagagcttc gtacataatc aacctgttcc ttgcggagtt
    32941 gccgcgtacc aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg
    33001 gtgtgaatac taaccaaagc gaatgacctt cgcaagggca attttatcgc attgtatcaa
    33061 ctgcggaaaa aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc
    33121 tttttagcca gttatctaac gctttaaacg gcttttactg catcggcgat acggtgtgca
    33181 aattcagtca cctgcgcttc gtcttcacct tccaccatca cgcgaattaa cggctcggtg
    33241 ccggatttac gcaacagcac acgtccacgg ctacccagcg cagcttcaac ctctgcggtc
    33301 acggctttaa ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgcacgtta
    33361 accagaatct gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta
    33421 cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta
    33481 tccagcagga tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt
    33541 ttttccagta cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc
    33601 agcgccagtt caagccccat gttgctcatc aatgtaccca cagcgccacc acgcagctgg
    33661 ccctgacgaa gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg
    33721 ccttcatggt caaccataat cacgcgatcg ccgtcgccgt caaaggcaat accgagatcc
    33781 gctttttcag ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca
    33841 ttgatgttta caccgttcgg ctcacaaccg atagcgataa cgttcgctcc cagttcacgc
    33901 agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt
    33961 tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgca
    34021 gcatcaacga tacggctggc tttacccagt tctgccgaat caacacagct gatctctttt
    34081 tccatttccg cttcgatggc ctcttctact gcatccggta gtttggtgcc gtcgatagag
    34141 aaaaatttaa tgccattatc gtagaatggg ttatgcgatg cagatatcac aatcccagcc
    34201 tctgcgcgga aagtccgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag
    34261 agcgcggaaa gaccggcagc cgccagaccc gcttccagtg ctgactccag catatagcca
    34321 gaaatacgtg tgtctttacc aataatgatc ttacgggagc cgtggcgcgc cagcacttta
    34381 cccgcggccc aacccagctt aagcacaaaa tcaggggtga tcggcgcatc ccctacacga
    34441 ccacgaatcc catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc
    34501 agacagagtg gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg
    34561 aatgatatgc gcgccttgca tagcggcaat gaccgcacaa gccagactac cgctcagacg
    34621 ctcggacggc cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa
    34681 cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga
    34741 gagattttta ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc
    34801 ctgctcgcaa cgtgctattt gctcaataaa atagcgattc acttctgcaa agacatcgtc
    34861 atacttcggg gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg
    34921 taaaccggtt tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcgtt
    34981 aataatgtga gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatctac
    35041 cgagatccag acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa
    35101 ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat
    35161 gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt
    35221 atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg
    35281 atggctaagg tccagtgaag taccctgggc aaagagtttc atgatgttat ccctggtatg
    35341 aattaataag aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct
    35401 gatgcgggaa cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta
    35461 cgcggttcat cgaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa
    35521 gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca
    35581 atctgcggtg cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata
    35641 tccatattgt cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc
    35701 acttcctggt cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta
    35761 cggccgagga acacttcacc ttcttcttcc gcgtacagca gtggccccaa tttctcagag
    35821 aagccccact gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggat
    35881 gcaccggtag aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac
    35941 agcgtagaaa tctggctttc cagtttctga cggctggcgc tgatagcgtc gccctcaggc
    36001 aagaagaaag tcacacccag agcacgaccg cgtgggataa tcgtcacttt gtgcaccgga
    36061 tcgtgttccg gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat
    36121 tctttctgcg cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct
    36181 ttcgctttct cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt
    36241 gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca
    36301 atgattgccg cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc
    36361 tgctcacgac cgcgaacatc tggcaaacca accacaacct gacggtcgaa acggccagga
    36421 cgcagcagcg ccgggtcgag aacgtccgga cggttagttg cggcgataac gatgatacct
    36481 tcgttacctt cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca
    36541 tcgtgaccac cgcccagacc tgcgccacgc tggcggccta cggcgtcgat ttcatcgata
    36601 aagatgatac acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca
    36661 cccacaccga cgaacatttc gacgaagtca gaaccggaga tagtaaagaa cggaactttc
    36721 gcttcgcctg caatcgcttt cgccagcagc gttttaccgg tacccggagg accgaccatc
    36781 agaacgcctt tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac
    36841 tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc
    36901 gttttgatct gatcttcagt cagcatgcgc gctttgctct taccaaacga catggcacct
    36961 ttgccaccgc cgccttgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc
    37021 atcgggaacc aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg
    37081 acaaccttga cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg
    37141 taagtggtat aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc
    37201 gcttcacgaa cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg
    37261 ccattagact cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc
    37321 agccagagta ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt
    37381 gttaaaaaca gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct
    37441 cccggttagg gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcacgagaa
    37501 gagtccggct tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca
    37561 tcgaaacctt cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca
    37621 cgacacattt ctagcgccag ttccaccaga tacatggcac gggggatatc caccgccggt
    37681 gttccgctca tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg
    37741 cgctccagca gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca
    37801 ccaacgatag gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt
    37861 tgggtgacca catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt
    37921 cccggtttaa agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa
    37981 cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag
    38041 cggctggagc tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca
    38101 tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta
    38161 gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca
    38221 aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa
    38281 tggtttgacc gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat
    38341 caaggtgaaa atcgccaccg aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt
    38401 gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc
    38461 aactaaagaa cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg
    38521 ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg
    38581 ataaaaggcc gcatagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt
    38641 gggtaattct tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt
    38701 tttgatgacc acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt
    38761 tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg
    38821 ttcttcgtca gaatcgagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt
    38881 gttgggcatt ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt
    38941 gatacggcct tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt
    39001 caggtcgcca tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga
    39061 tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc
    39121 ttgcatttgt tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg
    39181 ttcggcctgc ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt
    39241 acaagtccca ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga
    39301 agggtcatcg tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg
    39361 ttagcgcgag attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt
    39421 cagtgttcag gccgcaaatg ttgatgagta cattactcaa ctccccgctg gtgccaacct
    39481 tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca
    39541 gatggcgctg cccgccagta cgcagaaagt gattactgca ctggcggcgt tgattcaact
    39601 cggccccgat tttcgtttta ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt
    39661 acttaagggt gacttagtgg cgcgatttgg tgccgatccg acgttaagac gtcaggatat
    39721 tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt
    39781 tatagatacc tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat
    39841 gacacaatgc tttagcgctc cgcctgccgc cgccatagtt gaccgcaact gtttctccgt
    39901 ctcgctctac agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta
    39961 ccccgttacg atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata
    40021 ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt acgctgaccg gatgcctgcc
    40081 gcaacgttct gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg
    40141 ggcaattctg aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg
    40201 ccagactcag gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca
    40261 cgatctgctt aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg
    40321 catgataggc catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt
    40381 gcgtcagatc ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg
    40441 ttcagggctt tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata
    40501 cattgcccaa cacgacaatg aacttaactt tatctccatg ctgccgctgg cgggctatga
    40561 cggctctttg cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtatcagc
    40621 gaaaaccggt tcgttgcagg gggtatataa cctggcggga ttcattacca cggcgagcgg
    40681 gcaacgaatg gcgtttgtgc aatatctttc cggctatgca gtagaacccg cggatcagcg
    40741 taatcgccgt attccgttag tgcgatttga aagccgtttg tataaagata tttatcagaa
    40801 taattagtca aaaagaaacc ccggcgcatg gccggggttt tgctgattaa cgcttgtaaa
    40861 tgaactcaac gccttcttcg tcgtcttcgt cccagtcgtc atcccagtcc tcatcatcct
    40921 cttcagcaat ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct
    40981 ctggctgttt cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat
    41041 cccagcagag atctttcacg cccagtccgc tcgccgcaga gatcagataa tatttatctt
    41101 cccaacccag cgcctcagcg atcgctttcg ctttctcttc ggcttctgcc ttatccagca
    41161 ggtcgatctt gttgaagact aaccaacgcg gtttcgctgc cagatcctgg ctgtattttt
    41221 ccagctcgct gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga
    41281 tatcgatgag gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cgaatgccca
    41341 gacctgcgcc ttctgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct
    41401 tttcgttgtc cattcgtacc acaccaagac ttggcactag ggtggtaaac ggatagtccg
    41461 ccactttcgg tttagccgct gagacagcgc ggataaatgt cgatttaccc gcgtttggca
    41521 tccccaacat accgacgtca gccagcagca tcagctccag cagcagctcg cgcttatcgc
    41581 ccggcgtgcc gttggttttc tgccgtggcg tacggttaac ggacgatttg aagcgggtat
    41641 tgcccagacc gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttggtcatat
    41701 cgcccatggt ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaattg
    41761 tgacgtcttt accgcgctta ccggtacagt cgcggcttgc gccattctga ccacgctctg
    41821 cgcggaaaga tttttcaaaa cgataatcga taagcgtgtt caggttctcg tcggcttcca
    41881 tccatacgtc accgccgtca ccgccgtcgc cgccatccgg gccgcctttc ggaatatact
    41941 tttcgcggcg gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg
    42001 cttcatcaac aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata
    42061 ccaccgttgt atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg
    42121 cgcccgcaac cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga
    42181 agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga
    42241 gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc
    42301 catatcctac caatgtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa
    42361 gctgcgctat cactccaggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca
    42421 gtaaaaacag gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat
    42481 aactcaccca aaccgtcgcc gcaccgaccc caaagataac tccccaggtg taatcggtga
    42541 gctttgtaaa tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca
    42601 tcaatgcccc tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa
    42661 ccatcatgcc aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt
    42721 gcaaggatga gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca
    42781 aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg caacttcttc ttcaccgcaa
    42841 gaatggcacc aaggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt
    42901 ccatcacctc cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg
    42961 tgagcgccaa aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg
    43021 catttgcgga ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg taaaaagccc
    43081 cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga
    43141 tgctgataaa tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt
    43201 tagcaaacag agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac
    43261 cacgttgacg aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc
    43321 ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg
    43381 ccatttgaaa tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa
    43441 ccactgacga tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt
    43501 aactttctcg ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa
    43561 aggaacgccg attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac
    43621 agtttcgcca gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg
    43681 gtgttgttta ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac
    43741 cttttcaatg attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa
    43801 acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg
    43861 gtaacgttta tctgtgccat tttttcagta caatcaccct atattcctaa ccataaaccc
    43921 taagttgcct ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat
    43981 ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt
    44041 gagcagctta attccgacgt ccaactgatc aatcagttag gctattacat cgtcagcggc
    44101 ggcggtaaac gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag
    44161 ggaaatgcgc atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgctg
    44221 cacgacgacg ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgccgca
    44281 tttggcaatg ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg
    44341 atgaccagcc tcggctcgct caaagtgctg gaagtcatgt cagaagccgt aaacgtcatc
    44401 gcagaaggtg aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac
    44461 tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc
    44521 gggattctgg ctggctgtac gccggaggag gagaaaggcc tgcaggatta tgggcgctat
    44581 ctcggcactg ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag
    44641 ttaggtaaaa acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat
    44701 gcaatgcatc atggcacacc agaacaggca cagatgattc gtaccgccat cgaacagggt
    44761 aacggtcgcc atcttctgga accggttctg gaagcaatga acgcttgtgg atctcttgaa
    44821 tggacgcgtc agcgtgccga ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc
    44881 ccggacaccc cgtggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat
    44941 cgttaatccc ctccccgcat cccgcgcagc gcgcgggatg gttctagtaa attccataaa
    45001 aacacttatt cagctctata acacctgaaa aatagggctg tacatcttta tatattctga
    45061 atattcacac tctttacagg aactttttag aacaatacgc catcaggagt atagtgatgc
    45121 ccgacagaag aaatgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa
    45181 gtaatttcat tgactggcat cccgctgaca tcattgcggg tttgcgcaag aagggaacgt
    45241 caatggcggc ggaatctcgc agaaatggtt tgagttcctc aacgctggcg aacgcattat
    45301 cgcgcccctg gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctgga
    45361 ttatctggcc atctcgctac catgatccgc agacccatga gtttatcgac agaacgcagt
    45421 tgatgcgtag ctacactaaa ccgaaaaaat aagtggtctg gcggtagccc cgcgaacggg
    45481 gctgccagct ctcagacaat tactcgcctt tcacacgctc aatatttgca cctaaagcgc
    45541 gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg
    45601 tcgtcccttc cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg
    45661 ccataacctg tgcgccagaa agtttttcaa caccgtgaca aataacggta ttgctttcga
    45721 tttcggcgtg cgcgcccata cggctcagct ctggcacatg cataaagcgg ttttcaaaga
    45781 ccgtttcggt gatgaagccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct
    45841 gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt
    45901 tgccatgcat atccaggcta atccagtctt cgccgacttc gatgtctgct ccagcgtcac
    45961 gcagtttcgc cagcacggcg tccagagtat ctggctgcgc gttacggcag ataattttgc
    46021 cgcgagagat cgccgccgcc accaggaaag taccggtttc gatacgatcc ggcagcacgc
    46081 gatagacacc gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac
    46141 cgctaatttt cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac
    46201 gcgctgcgtt ttcaataatc gtggtgcctt ccgccagggt tgcagcacac atgatggtca
    46261 ccgttgcgcc aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga
    46321 cggaagcttt aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgagaccag
    46381 aaatgtgtag atcaaccgga cgtgcaccaa tcgtacaacc gccaggcagt gaaacttgcc
    46441 cctgaccaaa gcgcgctacc agcggcccca gcgcccagat agaagcacgc atggttttaa
    46501 ccagatcgta aggtgcgcag aatacattaa catcgcgggc atcaatatgc acagaaccat
    46561 tacgttctac tttcgcaccc agctggctta gcagcttcat tgatgtatcg acgtctttca
    46621 gtttcgggac gttctggatc tctaccggtt cttccgccag tagtgcggca aaaaggatag
    46681 gcagagcagc atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct
    46741 gaacacgaaa tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg
    46801 aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat
    46861 acgctttgat cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat
    46921 agaccgtctg ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa
    46981 cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca
    47041 acacgctctg aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg
    47101 aaacatctta gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca
    47161 gacaaggctc gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg
    47221 aaaaaattta acgaggcaga acatcagcag gcaaattata caatttcgcc agggtataca
    47281 ctttgtcgtt taccccctga agcgtcacgt tgttgccctg ctttttcgcc agatcgataa
    47341 gatggagcag cagtgccagt ccacccgtat ccacgcggga cacacggcta agatcgatgc
    47401 aggtaatccc cttcaccgct tcctcacgca tttcccaaag cggtagcaga acgtcctgat
    47461 ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca
    47521 ttattttttc tcttccagag tgattttctg ttgagaaatc gatttcagtt gtgcagtcag
    47581 gccgtcgata cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat
    47641 actgacgcct tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg
    47701 ccactggaag tccagacgca ccggcgggcg gccattcggg tcaataatgg taacgcgaat
    47761 aggcacaatg gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg
    47821 atacatcgcc agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta
    47881 agcatcacgt tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc
    47941 gtatttcacc tgtacgtatg gcaacagttc ctgatcgaca atggtacgca gataatccgg
    48001 gttggcccga atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc
    48061 gtccatcagc ttatacggat tggtctggtc tgccgcggtt gccgcactca gcggtgcaat
    48121 caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt
    48181 cgttgtaccc acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc
    48241 actattctta ttgtcatcgc ctttactacc gtaaaggaac tgaccaataa gatcttccag
    48301 caccatcgca gacttggtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag
    48361 ttccgggtct tcaaaaccga cgtttaatgc cagatattgt tcccccagca ggccggaagt
    48421 acgaatgctc agcgaactgg tatcaggaat gtggttataa cgttgttcaa tttccagcgt
    48481 tacgcgcggc agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac
    48541 accaccaatg ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata
    48601 aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa
    48661 cagcgccgcc agtagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt
    48721 ttgcatgaac tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag
    48781 ccagagacga gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga
    48841 tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct
    48901 taatcagaca gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga
    48961 agaacccgct atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca
    49021 cggcgacgaa aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag
    49081 aaataacccg acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag
    49141 cgcgcatcag gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg
    49201 cggcaaccac cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg
    49261 tttccgcact ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga
    49321 acacgccaga aactacaata atcagcatcg acaggacgcc gacattatag agctggcgca
    49381 ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta
    49441 acccggcccg cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg
    49501 catttaacag catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca
    49561 ggatagcgga acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga
    49621 tcaggattcg cctgcaacgc ctgggcacta ccatgagcga caattttttt gtccgccagg
    49681 atccaggcgt gatcggcaat acttaacact tccggcacat cgtgagaaac caccacacaa
    49741 gtcacaccca acgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg
    49801 ggatcttgcc caacaaaggg ttcatcaaac atgatgagat ccggctccag cgcaatcgca
    49861 cgcgccagcg ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc
    49921 gctccacgca gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg
    49981 ggaagttggg tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg
    50041 aacaacgccc cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa
    50101 cgagacatcg ccggaatatt ctcaccatcg aaaagaatct caccatgatc tggtgcgatt
    50161 tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc
    50221 gtgatcttcc ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc
    50281 gtaaaactga catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct
    50341 tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta
    50401 cagaatatta cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata
    50461 aagtcaaaat taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata
    50521 cctgaagaaa ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt
    50581 ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg
    50641 catcccgccg ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat
    50701 catcgtctcg cttgccgcgt ctttgcacga acaacgcgat ttagccgttg gtacagccct
    50761 cggctcaaac attatcaata tattgctgat cctcggtctg gctgcgctgg ttcgtccttt
    50821 taccgtccat tctgatgttc tacgccgtga attaccctta atgttgttgg tcagcgtggt
    50881 ggccggttcc gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt
    50941 tctggctgtg ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg
    51001 gactgacagc ctgaccagag agcagcttgc agagttgccg cctgacggtg gattgcccgt
    51061 cgcgttttta tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt
    51121 tgataacgcc acggtgctgg cgaattactt tgccatcagc gagttgacga tgggtctgac
    51181 ggcaattgct atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa
    51241 aggtgaaaac gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat
    51301 cgtgttgggt ttacccgcgc tgataacgcc aggagagatt gatccactgg cgtacagtcg
    51361 tgactacagc gtgatgttgc tggtgagcat tatttttgcg ttgctatgct ggcggcgctc
    51421 cccgcaaccg ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc
    51481 gatgttgtac tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg
    51541 tagagttaca accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac
    51601 gtgaatgcct ggcggagctt gatcaataca tcaatcagaa tttcacgctt gcctgtgaaa
    51661 agatgttctg gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcatattg
    51721 ggcgcaaaat ggcggcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg
    51781 gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca ggatgtggtg attgctatct
    51841 ctaactcagg tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg
    51901 taccgttaat ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgttc
    51961 atctgtgtgt taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca
    52021 ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct
    52081 ttactgccga agattttgcg ctctcacatc caggcggcgc actgggtcgt aaacttctgc
    52141 tgcgcgtaaa cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca
    52201 gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg
    52261 atgacaatat gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata
    52321 tgggcgtgga tgttcgtcag ttaagtattg ccgatgttat gacgccgggg ggaatacgcg
    52381 tgcgccctgg cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct
    52441 ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc
    52501 gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct
    52561 tgcgacctgt tacggccctg tcagcgccga cgttatagca aaagcagaga acattcgtct
    52621 gctgatcctc gatgtcgatg gcgtactgtc agatggcctg atttatatgg gcaataatgg
    52681 cgaagagctg aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc
    52741 tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc
    52801 cacattgggg atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga
    52861 tttgctggaa aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat
    52921 cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg cgcatccact
    52981 gttgctcccg cgcgccgatt acgtgacgcg cattgctggc ggtcgtggtg cagtgcgtga
    53041 agtttgcgac ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat
    53101 atgagtaaag ccagacgttg ggttatcatt gtgctatcac tggcggttct ggtgatgatc
    53161 ggcattaata tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc
    53221 acctataaaa gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat
    53281 cgattgattg ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag
    53341 ccggtactta ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa
    53401 gccaagctga ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactt
    53461 gtgccggact ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg
    53521 caggatgtta cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt
    53581 ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca
    53641 tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct
    53701 ggccgccagc attccggcat ttgccgtaac cggagacact gatcagccga tccacattga
    53761 atcggaccag caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat
    53821 cgtcacccag ggcaccatca aaattaatgc cgacaaagtg gtcgttaccc gtccgggcgg
    53881 cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca
    53941 ggacaatggt aaacccgttg aaggtcacgc ttcccagatg cactacgaac tggcaaaaga
    54001 ttttgtcgtt ctgacgggta atgcttatct gcagcaggtc gatagcaaca ttaagggcga
    54061 taagatcact tacctggtga aagagcagaa aatgcaggct ttcagcgaca aaggtaagcg
    54121 cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc
    54181 ggcacagaag aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa
    54241 agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat
    54301 tgtcggtctg ctggggccaa acggtgccgg taagaccacc actttctaca tggttgtagg
    54361 cattgtgccg cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc
    54421 tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgccg caggaagcct ccattttccg
    54481 tcgcctcagc gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc
    54541 tgaacaacgt gaagaccgcg cgaacgagct gatggaagag tttcacattg aacacttgcg
    54601 tgacagcatg gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc
    54661 actggctgcg aatccgaaat ttattctgct cgacgaaccg tttgccgggg ttgacccgat
    54721 ctcggttatc gacattaaac gcatcattga gcacctgcgc gacagcggcc tgggcgtact
    54781 gatcactgac cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag
    54841 tcaggggcat ttgatcgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa
    54901 gcgtgtatac cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag
    54961 caggagagta cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg
    55021 gcgatgacgc cacagctcca acaggcaatt cgtctgttgc agttgtcgac gctggaactt
    55081 cagcaggagc tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat
    55141 gaagaaatcg acacccgcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc
    55201 gaacaaaaag agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc
    55261 gctggtacac catccggcac cagcggtgac tacattgacg acgagctgcc ggtctatcag
    55321 ggcgaaacga cgcagacctt gcaggattac ctgatgtggc aggtcgagtt gacaccgttt
    55381 tctgacactg accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat
    55441 ctgactgtcc cactggaaga tattctcgaa agtatgggcg atgaagagat tgacatcgac
    55501 gaagttgaag ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt ggcagcaaaa
    55561 gatctgcgtg actgtctgct gatccaactc tcccaattcg ataaaaccac gccatggctg
    55621 gaagaggcca gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc
    55681 actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc
    55741 cagtcgctcg atccgcgccc cgggcagtcg atccagacgg gcgaacctga gtatgtcatt
    55801 ccagatgtgc tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc
    55861 attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat
    55921 ggtgacagcc agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg
    55981 gaaagccgta acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc
    56041 ttctttgagc aaggcgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct
    56101 gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca
    56161 cgaggcattt ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc
    56221 gaagcttcct ccacggcgat tcgtgcgctg gttaagaaat taatcgcggc ggaaaaccca
    56281 gcgaaaccgt tgagcgacag caagttaacc tctttgctgt cggaacaagg tatcatggtg
    56341 gcacgccgca ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt
    56401 aaacagctcg tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa
    56461 taacgtcgag atcaccgagg cactgcgcga atttgttaca gccaaatttg ccaaacttga
    56521 gcaatatttt gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca
    56581 cacctcagat gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca
    56641 ggacatgtac gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca
    56701 taaagataaa ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt
    56761 tgtgcggcac aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt
    56821 gggcaggttc ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt
    56881 tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag agcaaaaaac gcgccctgga
    56941 aatcatcagc gagctggcgg cgaaacaact tagtctgccg cctcaggtgg tttttgaagc
    57001 tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca
    57061 tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaaacgcc
    57121 tatagctttc gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc
    57181 ggcagaccaa actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga
    57241 caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat
    57301 tacggatacc gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg
    57361 ttgtgaggag aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa
    57421 atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt
    57481 agtgttgtta cccgatctgg ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag
    57541 cattgatgtt cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct
    57601 gcctgacgct ttctcaccgc aactactgtt cctggatgcc gaccgtaata ccttaattcg
    57661 tcgttacagt gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag
    57721 tgctatcgac aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt
    57781 cgatacctca gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg
    57841 taaacgtgaa cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc
    57901 tatcgatgca gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa
    57961 actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga
    58021 agtacacaat tttatctacc agacgcgaag ctatcttgag ctatggttac ctatgctgga
    58081 aaccaacaac cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc
    58141 ggtgtatatt gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc
    58201 acgccatcgt acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca
    58261 aacaagctgg gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcaaggtttt
    58321 gacgcagaag tgctgttacg taatgacgaa ggtaccgagg ccgaagccaa cagcgttatt
    58381 gcactgctga tgctggattc tgccaaagga cggcagattg aagttgaagc gaccggtcca
    58441 caggaagagg aagcactggc cgccgttatc gccctcttta attctggttt tgatgaagat
    58501 taatcttcat cactttgaca ttcaaatccc ttcaagctcc cccgacaata agaaaagcgc
    58561 gtactgaaat cgttctcaat caacgtcatt tgtacatttc gtgcgctttt cactttcaga
    58621 agaacattaa gaaaacctta agaagcattg tttaggtttc gtttaagtta atcgaccata
    58681 ctggagatcg tcagaaaata tttccaggag atggcatgat tcgcttatct gaacaaagtc
    58741 ccctgggcac cggaagacat cggaaatgtt atgcgcatcc ggaagatgcc caacgctgta
    58801 tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga gatccgccgt gagttaaagt
    58861 actacgcgca tcttggtcgc cggttaaaag actggagtgg aataccgcgc tatcacggta
    58921 ccgttgagac ggattgcgga accggttatg tctacgatgt gattgcagat tttgacggca
    58981 aaccctccat tacgctgacc gaatttgctg cacaatgccg ttacgaagaa gacattgcac
    59041 aactgcgcca gctactgaaa cagctgaagc gttatttgca ggataaccgt atcgtgacga
    59101 tgtcgctgaa gccgcagaat atcctctgtc accgcatcag tgagtcagag gttatccccg
    59161 tcgtctgcga caacattggt gaaagcacat tgattccact ggcgacctgg tcgaagtggt
    59221 gttgtctgcg taagcaagag cggttatgga aacgatttat cgcccaaccg gcgctgacta
    59281 ttgccctgca gaaagatctg caacctcgtg aaagcaaaac gctgaccctc acttcgcgcg
    59341 aagcttaatc cagctggtgt tgttgcataa acgactcacc gcctaactga tacatctgcc
    59401 gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg cgcggagact ttgaaacgaa
    59461 gtggattagg taatacagct gccagtaatg cagcttccga ccgggtaagc ttgctcgcgg
    59521 gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa cacaccgtcg ccaaattcgg
    59581 cgatattcag gtaaaccgtc aggatacgct ttttgctcca gaccgtttct atccccagcg
    59641 ttaatccggc ttccagccct tttcgaaccc agctacgccc atcccataaa aagagatttt
    59701 tggccgtctg ctgagaaatc gttgatgcac cgcgaatacg gttttcattg cgttcgttgt
    59761 gcgccagggc ttgctcaatg gaagcgacat caaagcccca gtgctcagga aacttctgat
    59821 cttctgcggc aatcaccgcc agtcccatcc acggcgagat ttgatccatg ttgacccagt
    59881 cagaatgtgc cacgtaacga aaattgccat gcagccaggc gctgacttgc cgctcgacca
    59941 ttaccgcaga gaaaggaaca ggcgcaacgc taaacaacgc aatgccccca ccccagaaaa
    60001 cggcgaggac aaccattaac cgcagaagaa aacggcgaat gaaactaaac accgttaagc
    60061 ggcttttact cattccgcca ggaccagcac gcgggaaacc agcttatcaa tgccgctcgc
    60121 cgcttctgca atgttctgcg ccagcatata tgccggggtg gtgacaatct tattgtcttc
    60181 atcaaccacg atatcatcga caggacacgg cacatgctcc gcgcccatct cttccagcac
    60241 ttctgcggta tcgatatcag taccgatggt caaacgcagc gggaaatcga aaattttcgg
    60301 cagcatcgcc ggggcaatac acataaaacc aagcggtttt ccggcctgat gcatcgcttg
    60361 tgccagcgcc tttaattcac ggtcaacggt gcattcgcta ccaagactgg caaaattgct
    60421 taaattcttc gccgcgccaa aaccccccgg cacaatcaac gcatccagtt cagcggcatc
    60481 ggcctgggcc agaggacgga tttcaccgcg cgttattcgt gccgcttcaa tcagcacatt
    60541 gcgcgtttcc gtcatcgctt cgcccgttaa atggttgata acatcaacct gctgcttatc
    60601 cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc
    60661 ttcatgaatt tcagaaccgt catagacgcc gcatccgctc agaattacgc caattttctt
    60721 cattgtgatc atcctttcgc aattgactga aacacattaa tttttttaat aaaaatgcta
    60781 cgcatcacac atttaactga ttcatgtaac aaatcattta agttttgcta tcttaactgc
    60841 gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc ataataattt acggcgcagc
    60901 caagatttcc ctggtgttgg cgcagtattc gcgcaccccg gtctagccgg ggtcattttt
    60961 tagtggcttt tgccacccac gctttcagta cttctacgtc gtgacgccac tcttctttca
    61021 tctcttcaat ccattcaccg acgttatctt cccaggcggg aaggtcagga gactgaattt
    61081 gctgacccag ctgttgcaga tggcgtaacc ccactgaccc cgccgcacct ttaattttat
    61141 gtccttcctc aacaatgcct tttttatcct gcgccgtcag attcgactcc agcacgctga
    61201 catagcccgg catcatcttc tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta
    61261 caagttcgag atactgttcc agcatgggaa tatcgagcaa tgcttctgat ttactgttct
    61321 cttctgtcgt caccgtactc tcctcatcat cctgggtatc ccagaatttc ttgatcatcg
    61381 cggttagcgc cggaacagaa agcggcttac tcagcacatc atccattcca gcattgaggt
    61441 actcttgctt gtctttcagc acgttagcgg ttaaggccac cagcggcggt aaatcctcgc
    61501 gcggataacg cttcgtcagt tcacgagaga tatccagccc ggtcatatct ggcaactgaa
    61561 tatccagcaa taccaggtcg tattcgcccg gtttaaacat ctccagcgcc gccttgccgg
    61621 tcatggcgac atcaacgctg ttacctaatt tttccagcac agaacgcgcg acaatcacgt
    61681 tcagttcaat gtcttccacc agcagcacat tcagcgccgg taaaggcata tcgtcttcat
    61741 caaacgcatc atcgacctct tcagccaccg acggtgcgtg gatcgtcaac gtaaaggttg
    61801 aacctttacc ctgttcgctg gtaaccgtaa tatcgccgcc catatttttc gccagacgac
    61861 gagaaacggc cagaccaata ccggtgccgg tggcaggttt accgccatga ctgtctttca
    61921 cctggtaata catggcaaag attttatcca gctcatcctg cggaatgccg ataccagagt
    61981 cttccacttc aaaatgcagc atatcgcctt catcgtagcg cacgcgcacg gtaacctggc
    62041 cttgctgggt gaatttgacg gcgttactga tgagattcca caggatctgc cgtaaacgcg
    62101 tcccgtcggt aatgacctga tgcggtaatg gcagcgtagg ctccagatta aagcgcaatc
    62161 ctttttgttg cgcctgcaat gcggagagat tttccagatc ggcaaggaag ctggtgaaat
    62221 caaccggctg attatcaagc tggaccttac gccgttccat cttatccatg tcgataatat
    62281 cgttgaagat attcccgagc gtgacggccg aaacatggat ggttttgaga tatttttcct
    62341 gctcggcggt gagttcggta tccagcagaa tgcggctcag gccgacgata ccattcagcg
    62401 gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt tttgtcgcgg ctggcccgtt
    62461 caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg accaaaaccc atcaaaccgt
    62521 gacgtttacc cacgcggtcg tagtacggca ctttacggat ttcaaaacag gctttgcgcc
    62581 cgtccgggta atccagccac tgttcatagg tcagtgacac attatgacgg aacacttttt
    62641 catcggtttc aatgactttt gcggcggctt ccggtgagta aacatcagca ggtttcaggt
    62701 gaaccagttg tttttcgctt tttccggtca gcagctccat cgcgcggtta cagccggaaa
    62761 actctttatc ttcgttacga taaaaaacca ggtcgggtga agcatcaaga aaggaacgta
    62821 agaatgagga ttgctgttcg agctgaattt gtgtctcttc gcgctcttta atttcaattt
    62881 tcagttggcc gaaggtttcc tgcagttctg cttccgcttt ttcacgaacg gcgatttcct
    62941 gatttagctg ggcaatatta tcttttaact gaacgttgag gctcaaatcg cgctcgcgca
    63001 tctcctccag tttttgcacc agccgtgaca gacgttgccg tgactcctcc agttgctcga
    63061 cgaccaccga tagaaagtag accgcccacg gcgtaatcag caaaccaaag aagatagaac
    63121 gaataacatc aatgctttcg acctgaccat gcagcaccat ggttaccgcc atttgtacca
    63181 caatggcaag aacgacgagg gccagcgcca gcaacattga gaagcgcacc agacctaact
    63241 tcatcatcag gtcaacataa tactgcgcca gcagacgaat ttgcttcatt agggaattcc
    63301 ttcacgacaa cctgccccaa taatacccaa ttctgacagt tacgttataa attgtgtgag
    63361 aaatgcggaa ttcccctcaa tggtctactc cgttggaggt agccacgggc gtcccagcgc
    63421 cgatccctgt acgccatgtt cattcagata gcgatccagc tcgaccattc ccgtccatcg
    63481 attttcgcac cacagcggag caagcaacgt cgggcgacgg gcgctggcgg aaatgcggtg
    63541 gtagatcact tccggtggcg tatggcgaat catttctccg gcagtgagcg tgtaatcctc
    63601 cagttcaata ccattcaaac gccccgcttc ccaggctttc gccataatgc tgccttttac
    63661 tatatgcagc ggatgcagct ttatgccgtc cacgccggtt tcaaccactc gctccagcgt
    63721 ttgcaaacat tcagcctgcc cttcgccagg caggccgaca attaagtgag aacacacctt
    63781 cagcccgcgc tcacgcgcca gctgcgtcgt gcgctgataa caggcaaaat catgaccgcg
    63841 attgatgcga tgtagcgttt tgtcgtgcgc ggtttgtagc cccagctcca gccacacttc
    63901 gtagccctgg tccttatatt cgcaaagcag atccaacacc gcatccggca cgcagtccgg
    63961 gcgggtacca acacacaaac cgacaatatt ggcctggctc accgcctgct gatacataga
    64021 acgcagcacc tgaacttccg caaaggtgct ggtatacgcc tgaaagtagg ccagatagcg
    64081 tttagcgcgg ttaactaaat tcgcctggtg cgccagttgt tcggcaatgg aacgatgttg
    64141 ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg cagccaccac gcccgatggt
    64201 accgtcacgg ttagggcagc taaaaccgcc gtgcagcgtc agcttatgca ccttttgccc
    64261 ataacgacgg gtgagatcac caccaaacat attgactaat ttctgtaact gcataatctg
    64321 atagaccgcg ccttgaaaag aggccaaagc ctgccatttt tagctcaatt cggcgatgac
    64381 ctggatcaat cgtcccggcc tgcttttatc aactgcataa tcaatcaaaa ttaccgaaat
    64441 ttcatgcata atcacataaa tcacttttgc ttatcttgag tcagattttt ttatctcctt
    64501 atggatttta ggcaaaaaca gtagcatgaa acgtcattac caattaaggc agtataaaat
    64561 gctggttttc tcgtcagttc aaggcaggat aagggttaac acgcctttat gacagtcagg
    64621 aattggctgt ttctctaacg actccccttt tgaccttaaa tagaaaatct attttaattt
    64681 cagttaatta ataaagaatt ttgcgctaaa gcacatttct gtaccaataa gcttgccatt
    64741 tgacctgtat cagctttccc gataagttgg aaatccgctg gaagctttct ggatgagcag
    64801 cctgctcatc atatttatgc agtaattgag atcccctctt caccgtatta accgatgcga
    64861 aaaggacaac aagggggcga atgcgaggcg cgcgtatgac acgcaacccc cgtcgccacg
    64921 ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt cccgcagagc ctgggggagg
    64981 ttcacgatat gttgtacgat aaatcccttg agagggataa ctgtggtttc ggcctgatcg
    65041 cccacataga aggcgaacct agccacaagg tagtgcgtac tgcaatacac gcactggccc
    65101 gcatgcagca ccgtggcgcg attctcgccg atggtaaaac cggtgacggt tgcggcttgc
    65161 tgttacaaaa accggatcgc ttttttcgca tcgttgcgca ggagcgcggc tggcgtttag
    65221 caaaaaacta cgccgtcggg atgctcttcc tgaataaaga tcctgaactc gctgctgccg
    65281 cacgccgcat cgttgaagaa gagctgcaac gcgaaacgtt gtcgattgtg ggctggcgtg
    65341 atgtccccac taacgaaggc gtgctgggtg aaatcgccct ctcctctctg ccacgcattg
    65401 agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg cgatatggag cgccgtctgt
    65461 ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga caaagacttc tacgtctgta
    65521 gcctgtcgaa tctggtgaac atctataaag gtctgtgtat gccggcggat ctgccgcgct
    65581 tctacctgga cctcgcggac ctgcgtctgg aatcggccat ttgcctgttc caccagcgct
    65641 tctccactaa caccgtaccg cgctggccgc tggcgcagcc gttccgctac ctggcgcata
    65701 acggtgaaat caacaccatc accggcaacc gccaatgggc gcgtgcgcgt acctataaat
    65761 tccagacgcc gctgatccct gacctgcacg acgccgcgcc gttcgtcaac gaaaccggtt
    65821 ctgactccag ttccatggat aacatgctgg aactcctgct ggcaggcggg atggatatca
    65881 tccgtgccat gcgtctatta gtgccacctg cctggcagaa caacccggat atggacccgg
    65941 aactgcgcgc cttctttgac tttaactcca tgcatatgga gccgtgggat ggcccggcag
    66001 gcatcgtgat gtccgatggt cgttttgccg cctgtaacct cgaccgtaac ggtctgcgtc
    66061 cggcgcgcta cgtcatcacc aaagataagc tcatcacctg cgcctctgaa gtcggtatct
    66121 gggattatca gcctgacgaa gtggtcgaaa aaggccgcgt ggggccaggc gaactgatgg
    66181 ttatcgacac ccgcagtggg cgcattctgc actcggcaga aactgatgac gatctgaaaa
    66241 gccgccatcc atataaagag tggatggaga aaaacgtccg ccgactggta ccgtttgaag
    66301 atctgcccga tgaagaagtg ggtagccgcg aactggacga cgacacgctt gccagctacc
    66361 agaaacagtt taactacagc gcggaagagc tggactccgt aattcgcgta ctgggcgaaa
    66421 acggtcagga agcggtcggt tcgatgggcg acgacacccc attcgccgtg ctctccagtc
    66481 agccgcgcat tatttacgat tacttccgcc agcaatttgc ccaggtcacc aacccgccaa
    66541 tcgacccact gcgtgaagcg catgttatgt cgctcgccac cagcatcggg cgtgaaatga
    66601 acgtcttctg tgaagcagag ggccaggcgc accgtttaag ttttaaatcg ccgattctgc
    66661 tctactccga tttcaaacag ctcacgacga tgaaagagga gcactaccgc gcagatacac
    66721 tggatatcac ctttgacgtc accaaaacca cgctcgaagc gacggtcaaa gagctgtgcg
    66781 acaaagccga aaaaatggta cgtagcggca ctgtgctgct ggtgctctcc gaccggaata
    66841 tcgctaaaga tcgcctgccg gttccggctc cgatggcggt tggcgcgatc cagacccgtc
    66901 tggtcgatca aagcttgcgt tgcgatgcca acatcatcgt cgaaaccgcc agcgcccgcg
    66961 atccgcacca tttcgccgtg ttgctgggct tcggtgcgac ggctatttat ccgtaccttg
    67021 cctatgaaac gctgggccgc ctggtagaca cccacgcgat tgccaaagat tatcgtaccg
    67081 tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta caaaatcatg tccaaaatgg
    67141 gcatctccac catcgcctct taccgctgct cgaaactgtt tgaagcggtc ggtctgcacg
    67201 atgatgtcgt aggcctgtgc ttccagggcg ctgttagccg cattggcggg gcgagctttg
    67261 aagacttcca gcaggatctg ctgaatctgt cgaaacgtgc ctggctggcg cgcaagccca
    67321 tcagccaggg cggcctgctg aaatacgtgc acggcggcga ataccacgcc tacaacccgg
    67381 acgtggtgcg cacactgcag caggcggtac aaagcggcga gtatagcgac tatcaggaat
    67441 acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg cgatctgctg gcaattacgc
    67501 cgggtgaaaa cgcagtcaac attgccgatg ttgaaccggc aagcgaactg tttaaacgct
    67561 tcgataccgc tgcgatgtct atcggcgcgt taagcccgga agcccacgag gcgctggcgg
    67621 aagcgatgaa cagcatcggc ggtaactcga actccggtga aggcggcgaa gatccggcgc
    67681 gctacggcac taacaaagtg tcgcgcatca agcaggtggc gtccggtcgc ttcggtgtta
    67741 ctccggcgta cctggtcaat gccgacgtca ttcagattaa agtcgcccag ggcgcgaagc
    67801 cgggcgaagg cggtcaattg ccgggtgata aagtcactcc ttacattgcc aaactgcgct
    67861 attcggtgcc cggagtgacg ctgatctccc cgccgccgca ccacgatatc tactctatcg
    67921 aggatttagc gcagctgata ttcgacctca agcaggtcaa tccaaaagcg atgatctccg
    67981 tgaagctggt ttccgaaccg ggcgtaggca ccatcgcgac tggcgtggca aaagcttatg
    68041 ccgatttaat caccatcgca ggctatgacg gcggcaccgg cgcaagtccg ctttcatcgg
    68101 tgaaatacgc aggctgtccg tgggaactcg gcctggtgga aacccaacag gcgctggttg
    68161 ccaacggttt acgtcataaa attcgtttgc aggttgatgg cggcctgaaa acgggcgttg
    68221 atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg cttcggcact ggcccaatgg
    68281 tcgcgctcgg ttgtaaatat ctgcgaattt gccacctgaa caactgcgcc acgggtgtag
    68341 caactcagga tgacaaactg cgtaagaatc actatcacgg cctgccgttc aaagtgacga
    68401 attactttga gtttatcgcc cgtgaaaccc gcgagctgat ggcacagctg ggcgtaacac
    68461 gtctggtgga tctgattggt cgcaccgacc tgctgaaaga gctggacggt ttcaccgcca
    68521 aacagcagaa actggcgctg tcgaagctgc tggagaccgc cgagccacat gcaggtaagg
    68581 cactctactg caccgaaaac aacccgccgt ttgataacgg cctgctgaac gcgcagttgc
    68641 tgcaacaggc aaaaccgttt gttgatgaac gccagagcaa aaccttctgg ttcgatatcc
    68701 gcaacaccga ccgttctgtc ggcgcatcgc tttcaggcta tatcgcccag acgcacggcg
    68761 atcagggtct ggcagccgat ccgatcaaag cgtacttcaa cggcaccgca ggccagagct
    68821 tcggtgtgtg gaacgcgggc ggcgtggaac tgtacctgac cggtgatgcc aacgactatg
    68881 tcggtaaagg catggcgggc ggcttaatcg ccattcgccc tccggttggc tcagccttcc
    68941 gcagccatga agcaagtatt atcggcaaca cctgcctgta tggcgcgacc ggtggtcgtc
    69001 tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg gaactccggt gctatcaccg
    69061 tggtagaagg cattggcgac aatggctgtg aatatatgac gggcggtatt gtctgtattc
    69121 tgggtaaaac cggcgtgaat ttcggcgcgg gtatgaccgg aggcttcgct tacgttctcg
    69181 atgaaagcgg cgatttccgc aaacgcgtta acccggaact ggtcgaggtc ttaagcgttg
    69241 acgatctggc gatccatgaa gagcatctgc gcggtcttat caccgagcat gtgcagcata
    69301 ccggttccca gcgcggtgaa gagattctgg cgaactggtc aaccttcgcc acgaaattcg
    69361 cgctggttaa accgaagtcc agtgatgtaa aagcactgct gggtcaccgt agtcgtagcg
    69421 cagcagagtt gcgcgtgcag gcgcagtaag gggtagcaac aatgagtcag aatgtttatc
    69481 aatttatcga cctgcagcgc gttgatccgc caaagaaacc gctgaagatc cgcaaaattg
    69541 agtttgttga aatttacgag ccgttttccg aaggccaggc caaagcgcag gctgaccgct
    69601 gcctgtcgtg cggcaaccca tactgcgagt ggaaatgccc ggtacacaac tacatcccga
    69661 actggctgaa gctcgccaac gaggggcgta tttttgaagc ggcagaactg tcgcatcaga
    69721 ccaataccct gccggaagtg tgcggacgtg tctgcccgca agaccgtctg tgcgaaggtt
    69781 cctgcaccct gaacgatgag ttcggcgcag tgaccatcgg caacattgag cgctatatca
    69841 acgataaagc gttcgagatg ggctggcgtc cggatatgtc cggcgtgaaa caaaccggta
    69901 aaaaagtggc gattatcggc gcaggcccgg cgggtctggc gtgcgcggat gtcctgacgc
    69961 gcaacggcgt aaaagcggtc gtcttcgacc gtcacccgga aattggcgga ttgctgacct
    70021 tcggtattcc ggctttcaag ctggaaaaag aggtaatgac gcgccgccgt gaaatcttca
    70081 ccggcatggg tattgaattc aaactcaata ccgaagtggg ccgcgacgta cagctggacg
    70141 atctgctgag tgattacgat gccgtgttcc ttggcgtcgg gacttatcag tcaatgcgcg
    70201 gcgggctgga aaacgaagac gccgatggcg tgtacgcagc gctgccgttc cttatcgcca
    70261 acaccaaaca gttaatgggc tttggcgaaa cccgcgaaga accgttcgtc agcatggaag
    70321 gcaaacgcgt ggtggtactt ggcggtggcg acactgcgat ggactgcgtg cgtacgtctg
    70381 tacgccaggg agcgaagcac gttacctgtg cctatcgtcg tgatgaagag aacatgccag
    70441 gttcccgccg cgaagtgaaa aacgcgcgag aagaaggtgt agagttcaaa ttcaatgtcc
    70501 agccactggg gattgaagtg aacggtaacg gcaaagtcag cggcgtaaaa atggtacgta
    70561 ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc ggagatcgtg gcaggttccg
    70621 aacatatcgt tccggcagat gcggtgatca tggcgtttgg tttccgccca cacagcatgg
    70681 aatggctggc gaaacacagc gtcgagctgg attcgcaagg tcgcatcatc gccccagaag
    70741 gcaacgacaa tgccttccag accagtaacc cgaaaatctt tgctggcggc gatatcgtcc
    70801 gtggttccga tctggtcgtt accgccatcg ccgaaggtcg caaagcggca gacggcatta
    70861 tgaactggct ggaagtttaa gctctgtccc agcgccgcct cctcagaaaa gagtcggcgc
    70921 tgaaattctc ctttcgttct ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata
    70981 cgcacatctg gattgttacc ggcagatgcc cggcgtatag ctgattccat tattcaacac
    71041 tgcgccatat taagggacag ataatggaat cgctctcaga aggaaccaca gcaggctacc
    71101 agcaaatcca cgacggcatt attcatctgg tcgatagcgc ccgaacggaa acggtacgta
    71161 gcgttaacgc gttaatgacc gcgacgtact gggaaattgg ccgacgaatt gtcgaatttg
    71221 aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg
    71281 atttaagtct aaggtataag cgtggatttt ccaccaggaa tttatggcag tttaagaatt
    71341 tttatatttg ttttcaaaga attgaaattg tgcagacact gtctgcacaa ttcaccccca
    71401 caattctcca gactgtgtct ggagaatctt gccatttagc gcaactcgcc aaatccttcc
    71461 ccctgccctg gtccacttac gtccgtttgc tttctgttaa aaaagctgat gctcgcagct
    71521 tttatgagaa agagacactc cgctgtggct ggtctgctcg tcagctcgaa cgacaaattg
    71581 cgacccagtt ttatgagcgg acactactgt cacatgacaa atcagccatg ctgcaacaac
    71641 acgctcctac cgagacgcat attcttccgc agcaggcgat acgcgatccc tttgtgctcg
    71701 aatttctgga attgaaagat gaatactcag aatccgattt tgaggaggcg ctgatcaacc
    71761 acctgatgga tttcatgctg gaacttgggg atgattttgc ctttgttggt cgacagcgaa
    71821 ggttacgcat tgatgacaac tggtttcgtg tcgatctgct gtttttccac cgccgtttac
    71881 gctgcctgct aatcgtcgat ctaaaagtgg gcaaattcag ctatagcgat gccggacaga
    71941 tgaatatgta tctcaactac gccaaagagc actggacact accggatgaa aatccgccca
    72001 tcggtctggt tctctgtgca gagaaaggag ccggcgaagc gtattatgca ctggctggtt
    72061 tgcctaacac cgttctggcg agcgaatata agatgcaact acctgatgaa aaacgactcg
    72121 cggatgaact cgttcgaaca caggcggtgc tagaggaagg ctatagacgc cgttaatttc
    72181 agcggtttcg aattctgcag acagtgtctg cagaatcttt aaaacgaact aagttttaag
    72241 ccattaaatc agccttaacc ttcaccacaa cctttttaat ctcatcaggt tcgccgacaa
    72301 cgcatcctgg tttatgcggt tctcccggca taaacacggc gaacattccc ggttttaaga
    72361 tgatggcttg ctcgttctca atggcgctgc aaagctggta atcatcctca tggtggaact
    72421 cttcacactg acgcgcagtg cctgccgtgc caaacagaat ccgttcctca ccgtttaaca
    72481 acagctggat atcaatgtat tgctcgtgca attccgcttt tttctcgacg ggcgattgag
    72541 tgttaaacgt catgacattc ataaagatat tgtcgccctg taattcgtaa cgacccggtg
    72601 ctttttcttg cggcctggcg gctaaggcta gtgttaacgc gtcctgtaac gcaggatgca
    72661 atccggcaga cggtaatgac tgtacttcac ccatcatcat aatttttctc cctgggccaa
    72721 cagcgcagcc ccaagtaaac ctgcatcgtg gcggtagtgc gccgccagta aatcaacatg
    72781 aaatgccgct ggctcctgcg ccagatacgt ttccaccagc gccagatacc cctctgccag
    72841 accaacgctg ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat
    72901 cagccttgca agcgtacgtg cggaacggtg aatcagttgc tgcgcctgct catcgccctg
    72961 cccggcatgc gtgaaaatag ttttcgcatt cgcaccagcc aactcccctt gcgccgccgc
    73021 cgcaatgccg cgaccagaag caatcgcctc cacgcaacct gtgcgtccac agccgcagac
    73081 tggaccatgc ggatcggcaa gcgtatgccc gatgtgcccc gccagaccgc cagggccggt
    73141 aagcagtttg ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaacac
    73201 catctcggtt acatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc
    73261 gttaatggca atggtcggca aattggtaag ttgttccagc gttttgacta acggaaagtg
    73321 tagtaatcca ccgagattat gcggattaag cgccagcaaa ctgccgtcgc gaataattcc
    73381 ggttgaagcg atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa
    73441 ggcatcacgc aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg
    73501 gatctgccca tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc
    73561 cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca
    73621 agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatccgcc
    73681 gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc gacatccggc gtcgctcaac
    73741 gttttcacca gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc
    73801 gtagtgccaa taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag
    73861 cagtcggtca tcgccagtaa accgtgatgg tgaatacgtg ccagcaacgt ttcaacaggc
    73921 accggacgcg ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc
    73981 gcatcaacat cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc
    74041 acaatcccga taattggcac gctcactacc gcacgcgtgg cttgcagatt tgccacacct
    74101 tcaatgcgaa tggcaaccgc gcccgcctgc tctgccgcta atgccatggc ggcgacgatt
    74161 tcgggtttat cgagcgggct gtccggaacc ggctggcagg agacaatcag gccaccgtta
    74221 gcagcgattt tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc
    74281 ccggtacaga gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc
    74341 ggccaaatgg cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg
    74401 cttccgggcg cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca
    74461 ccacaaatgt cagactgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag
    74521 cgatcaacgc gccgatgatt ggggccagtg caccgcccaa tgcgccgacg ttgtaggtaa
    74581 agcccaggcc cgctgcacgc tggtcggtat cgaaataacc gccaatcagt ttcggtaaga
    74641 tcccggcgat cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga
    74701 cgtttgcgcc gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcaggctac
    74761 aaacgtacgc tttgcgggtt cccagccagt caccgaggaa gccacctacg cagcatccca
    74821 ccgccgcgcc aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca
    74881 gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa
    74941 acaacacgac caccatcagc attacgcccg ttggccagcg tttgcccgta ctttgcacca
    75001 taaagctgat aaagattgcg gcgcataaca gcccaagaac agcgacgatc gcggcatttt
    75061 gcaggttacc ggcgaagcaa aaccacagcg cagtagccgc cgccagtgtc attacgatat
    75121 tggcaatgcg atgttcacca cggtagagaa tatctaccat cgtgcgtact ggtgctttac
    75181 cagcatgttt ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa
    75241 agatgattgg caaaatgccg ataaagaaca gcgcacgcca gccccagacc ggaaccacca
    75301 ggctatagac ctgagcggca acgacggccc ccacagagaa gccggaaatc agaaaaccac
    75361 tggctttatt acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt
    75421 attcacccgc catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg
    75481 gcgcaaagcc gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca
    75541 gacgacgccc gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga
    75601 taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccttgta
    75661 cttcggtgag taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc
    75721 aggcagcgga aaatgcccgc cattgtgcac gattgagatg gcgataccac gggatattct
    75781 gggttgaagt actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga
    75841 aaattgtagg gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa
    75901 caactcaccc gcgctcttgc atcaactgct gggccaacgc cttcagttct ggcagatatt
    75961 tttcatctac cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    76021 gcaggaccgt tttcaggccg cggaatacgc ccgttttgat cagtaaatca atgactttat
    76081 tacattcggt ttgcagcttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacaa
    76141 tcccctgata acgccagccc atgatgttgt aggtactacc gataccacca tcagcgcccg
    76201 ccagaagacc agaggcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt
    76261 cacgacggat ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc
    76321 caggcaacgt aacaagtgtg ttgatttgat ccagggtcag ttttacccca ctcagggctg
    76381 gaatgttgta caccaccatc ggcaacccat ccgctgaatc aataattgcc cgatagtgat
    76441 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    76501 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    76561 cgtgggcgat gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt
    76621 cacgctcgga aaggctttgt acaaaggctt cgccggtcga accacccacg tataaaccgt
    76681 caatgccctg ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt
    76741 gttgttgatc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    76801 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    76861 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat
    76921 tatttctttt tgttggtggt ctgaccgaag gcgtgccagg tagcagagac gctgttgaga
    76981 tgcgattgca acgcacgatc ggcttcgtca ggatcatgac gccggatcgc gtcaacgatc
    77041 gcaatatgct gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt
    77101 ggacgtgcgg cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    77161 ccggggatct ccgccagaac gcggtggaaa tcaacgtctg agcgaatgaa tgccgcgttg
    77221 ttatccagcg actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    77281 gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    77341 aaatgggcaa tcccgccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    77401 atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt cgccgttgtt tatctgcacc
    77461 agacctttgc gttttaacgc tgccagcgct tcgcgcaccg aaggacgccc gacgttaaag
    77521 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    77581 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    77641 ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcag tttgcgaatc aaatgcgttc
    77701 ataaggccca ttctgtaagg tcagt
//
LOCUS       DARNVM010000003        76923 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.003, whole genome shotgun sequence.
ACCESSION   DARNVM010000003 DARNVM010000000
VERSION     DARNVM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.003"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            351..854
                     /gene="dps"
                     /locus_tag="SI621_000152"
                     /gene_synonym="pexB"
     CDS             351..854
                     /gene="dps"
                     /locus_tag="SI621_000152"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEI4062921.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1258..2004
                     /gene="glnH"
                     /locus_tag="SI621_000153"
     CDS             1258..2004
                     /gene="glnH"
                     /locus_tag="SI621_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HEI4062922.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2143..2802
                     /gene="glnP"
                     /locus_tag="SI621_000154"
     CDS             2143..2802
                     /gene="glnP"
                     /locus_tag="SI621_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HEI4062923.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2799..3521
                     /gene="glnQ"
                     /locus_tag="SI621_000155"
     CDS             2799..3521
                     /gene="glnQ"
                     /locus_tag="SI621_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HEI4062924.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3638..5863
                     /gene="ybiO"
                     /locus_tag="SI621_000156"
     CDS             3638..5863
                     /gene="ybiO"
                     /locus_tag="SI621_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HEI4062925.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLSAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEDIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAAPLPPGEPT
                     L"
     gene            complement(5860..6786)
                     /gene="rlmF"
                     /locus_tag="SI621_000157"
     CDS             complement(5860..6786)
                     /gene="rlmF"
                     /locus_tag="SI621_000157"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HEI4062926.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSVQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7062..7322
                     /locus_tag="SI621_000158"
     CDS             7062..7322
                     /locus_tag="SI621_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4062927.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7587..9869
                     /locus_tag="SI621_000159"
     CDS             7587..9869
                     /locus_tag="SI621_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HEI4062928.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9911..10588
                     /gene="ybiX"
                     /locus_tag="SI621_000160"
     CDS             9911..10588
                     /gene="ybiX"
                     /locus_tag="SI621_000160"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HEI4062929.1"
                     /translation="MMYHIPGVLSPKDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10662..10928
                     /gene="ybiI"
                     /locus_tag="SI621_000161"
     CDS             10662..10928
                     /gene="ybiI"
                     /locus_tag="SI621_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HEI4062930.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            11193..11453
                     /locus_tag="SI621_000162"
     CDS             11193..11453
                     /locus_tag="SI621_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4062931.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11593..12678)
                     /gene="hcxB"
                     /locus_tag="SI621_000163"
     CDS             complement(11593..12678)
                     /gene="hcxB"
                     /locus_tag="SI621_000163"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HEI4062932.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(12819..13781)
                     /gene="ybiB"
                     /locus_tag="SI621_000164"
     CDS             complement(12819..13781)
                     /gene="ybiB"
                     /locus_tag="SI621_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEI4062933.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(13809..15959)
                     /gene="dinG"
                     /locus_tag="SI621_000165"
     CDS             complement(13809..15959)
                     /gene="dinG"
                     /locus_tag="SI621_000165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEI4062934.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVGNGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(16130..16411)
                     /locus_tag="SI621_000166"
     CDS             complement(16130..16411)
                     /locus_tag="SI621_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEI4062935.1"
                     /translation="MRLEWKPMSLSDREQIMEYISKDNPLAAIALDNQFEAIAEQACE
                     RPEMYRAGRVAGTHEAVVRPHYVLVYQVEGDTLSVLRVLHTALQWPPAE"
     gene            complement(16411..16851)
                     /locus_tag="SI621_000167"
     CDS             complement(16411..16851)
                     /locus_tag="SI621_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4062936.1"
                     /translation="MAPETIDHSTLHKLVESGVVDAAHVIGTQGGWSLTVKYGHTERP
                     LAAQRSRQIRLFKRLETVVNYLKDVGIARFEVDASNYDPDGQKKTTRPDRAEALKRAH
                     EAAAYDAWFREQVQAAIDDPRPALSHEEAKSLFAARKKALLKGD"
     gene            complement(17008..18372)
                     /gene="rhlE"
                     /locus_tag="SI621_000168"
     CDS             complement(17008..18372)
                     /gene="rhlE"
                     /locus_tag="SI621_000168"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HEI4062937.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            18601..19272
                     /gene="cecR"
                     /locus_tag="SI621_000169"
     CDS             18601..19272
                     /gene="cecR"
                     /locus_tag="SI621_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HEI4062938.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            19275..20270
                     /gene="hlyD"
                     /locus_tag="SI621_000170"
     CDS             19275..20270
                     /gene="hlyD"
                     /locus_tag="SI621_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HEI4062939.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            20263..21999
                     /locus_tag="SI621_000171"
     CDS             20263..21999
                     /locus_tag="SI621_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4062940.1"
                     /translation="MNDAVITLNGLEKRFPGMNKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFSRLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21992..23125
                     /locus_tag="SI621_000172"
     CDS             21992..23125
                     /locus_tag="SI621_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4062941.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            23136..24242
                     /locus_tag="SI621_000173"
     CDS             23136..24242
                     /locus_tag="SI621_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4062942.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(24204..24614)
                     /locus_tag="SI621_000174"
     CDS             complement(24204..24614)
                     /locus_tag="SI621_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HEI4062943.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24747..25508
                     /locus_tag="SI621_000175"
     CDS             24747..25508
                     /locus_tag="SI621_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEI4062944.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            25505..26746
                     /gene="clsB"
                     /locus_tag="SI621_000176"
     CDS             25505..26746
                     /gene="clsB"
                     /locus_tag="SI621_000176"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEI4062945.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDAHIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26746..27702
                     /locus_tag="SI621_000177"
     CDS             26746..27702
                     /locus_tag="SI621_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEI4062946.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27738..28127)
                     /locus_tag="SI621_000178"
     CDS             complement(27738..28127)
                     /locus_tag="SI621_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4062947.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGMVATAELALVIIMD
                     AALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(28332..29036)
                     /locus_tag="SI621_000179"
     CDS             complement(28332..29036)
                     /locus_tag="SI621_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEI4062948.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(29173..29625)
                     /gene="moaE"
                     /locus_tag="SI621_000180"
     CDS             complement(29173..29625)
                     /gene="moaE"
                     /locus_tag="SI621_000180"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEI4062949.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(29627..29872)
                     /gene="moaD"
                     /locus_tag="SI621_000181"
     CDS             complement(29627..29872)
                     /gene="moaD"
                     /locus_tag="SI621_000181"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HEI4062950.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(29865..30350)
                     /gene="moaC"
                     /locus_tag="SI621_000182"
     CDS             complement(29865..30350)
                     /gene="moaC"
                     /locus_tag="SI621_000182"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEI4062951.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(30353..30865)
                     /gene="moaB"
                     /locus_tag="SI621_000183"
     CDS             complement(30353..30865)
                     /gene="moaB"
                     /locus_tag="SI621_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEI4062952.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(30887..31876)
                     /gene="moaA"
                     /locus_tag="SI621_000184"
     CDS             complement(30887..31876)
                     /gene="moaA"
                     /locus_tag="SI621_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEI4062953.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDAHQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(31864..32008)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            32273..33181
                     /gene="yvcK"
                     /locus_tag="SI621_000185"
     CDS             32273..33181
                     /gene="yvcK"
                     /locus_tag="SI621_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEI4062954.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLNEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(33334..35355)
                     /gene="uvrB"
                     /locus_tag="SI621_000186"
     CDS             complement(33334..35355)
                     /gene="uvrB"
                     /locus_tag="SI621_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEI4062955.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(35934..36611)
                     /gene="bioD"
                     /locus_tag="SI621_000187"
     CDS             complement(35934..36611)
                     /gene="bioD"
                     /locus_tag="SI621_000187"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEI4062956.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(36604..37359)
                     /gene="bioC"
                     /locus_tag="SI621_000188"
     CDS             complement(36604..37359)
                     /gene="bioC"
                     /locus_tag="SI621_000188"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEI4062957.1"
                     /translation="MTTVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(37346..38500)
                     /gene="bioF"
                     /locus_tag="SI621_000189"
     CDS             complement(37346..38500)
                     /gene="bioF"
                     /locus_tag="SI621_000189"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEI4062958.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKLSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDSLLEVLHDNG"
     gene            complement(38497..39537)
                     /gene="bioB"
                     /locus_tag="SI621_000190"
     CDS             complement(38497..39537)
                     /gene="bioB"
                     /locus_tag="SI621_000190"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEI4062959.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39624..40913
                     /gene="bioA"
                     /locus_tag="SI621_000191"
     CDS             39624..40913
                     /gene="bioA"
                     /locus_tag="SI621_000191"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEI4062960.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRLDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            40972..41448
                     /locus_tag="SI621_000192"
     CDS             40972..41448
                     /locus_tag="SI621_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HEI4062961.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLARASITAMFS"
     gene            41600..42883
                     /locus_tag="SI621_000193"
     CDS             41600..42883
                     /locus_tag="SI621_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HEI4062962.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLESNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGNVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(42953..45214)
                     /locus_tag="SI621_000194"
     CDS             complement(42953..45214)
                     /locus_tag="SI621_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEI4062963.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGSIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(45397..46830)
                     /locus_tag="SI621_000195"
     CDS             complement(45397..46830)
                     /locus_tag="SI621_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEI4062964.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGGDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(46906..47958)
                     /locus_tag="SI621_000196"
     CDS             complement(46906..47958)
                     /locus_tag="SI621_000196"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEI4062965.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFTEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPTELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            48142..49095
                     /locus_tag="SI621_000197"
     CDS             48142..49095
                     /locus_tag="SI621_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4062966.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKVGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLVKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(49136..50131)
                     /gene="pgl"
                     /locus_tag="SI621_000198"
     CDS             complement(49136..50131)
                     /gene="pgl"
                     /locus_tag="SI621_000198"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEI4062967.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            50286..51104
                     /gene="ybhA"
                     /locus_tag="SI621_000199"
     CDS             50286..51104
                     /gene="ybhA"
                     /locus_tag="SI621_000199"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HEI4062968.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLAKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIARFIYSHLI"
     gene            complement(51105..52163)
                     /gene="modC"
                     /locus_tag="SI621_000200"
     CDS             complement(51105..52163)
                     /gene="modC"
                     /locus_tag="SI621_000200"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HEI4062969.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(52166..52855)
                     /gene="modB"
                     /locus_tag="SI621_000201"
     CDS             complement(52166..52855)
                     /gene="modB"
                     /locus_tag="SI621_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEI4062970.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(52855..53628)
                     /gene="modA"
                     /locus_tag="SI621_000202"
     CDS             complement(52855..53628)
                     /gene="modA"
                     /locus_tag="SI621_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4062971.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVIAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(53795..53944)
                     /gene="acrZ"
                     /locus_tag="SI621_000203"
     CDS             complement(53795..53944)
                     /gene="acrZ"
                     /locus_tag="SI621_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HEI4062972.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            54073..54861
                     /gene="modE"
                     /locus_tag="SI621_000204"
     CDS             54073..54861
                     /gene="modE"
                     /locus_tag="SI621_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HEI4062973.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAMLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAIAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            54929..56401
                     /gene="modF"
                     /locus_tag="SI621_000205"
     CDS             54929..56401
                     /gene="modF"
                     /locus_tag="SI621_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HEI4062974.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            56664..57680
                     /gene="galE"
                     /locus_tag="SI621_000206"
     CDS             56664..57680
                     /gene="galE"
                     /locus_tag="SI621_000206"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEI4062975.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            57690..58736
                     /gene="galT"
                     /locus_tag="SI621_000207"
     CDS             57690..58736
                     /gene="galT"
                     /locus_tag="SI621_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEI4062976.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESKDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDGQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            58740..59888
                     /gene="galK"
                     /locus_tag="SI621_000208"
     CDS             58740..59888
                     /gene="galK"
                     /locus_tag="SI621_000208"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEI4062977.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            59882..60922
                     /gene="galM"
                     /locus_tag="SI621_000209"
     CDS             59882..60922
                     /gene="galM"
                     /locus_tag="SI621_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HEI4062978.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGSTLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            61125..61877
                     /gene="gpmA"
                     /locus_tag="SI621_000210"
     CDS             61125..61877
                     /gene="gpmA"
                     /locus_tag="SI621_000210"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEI4062979.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(62043..63095)
                     /gene="aroG"
                     /locus_tag="SI621_000211"
     CDS             complement(62043..63095)
                     /gene="aroG"
                     /locus_tag="SI621_000211"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEI4062980.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGTPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            63411..63791
                     /locus_tag="SI621_000212"
     CDS             63411..63791
                     /locus_tag="SI621_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HEI4062981.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            63905..64846
                     /gene="zitB"
                     /locus_tag="SI621_000213"
     CDS             63905..64846
                     /gene="zitB"
                     /locus_tag="SI621_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HEI4062982.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREISEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(64843..65562)
                     /gene="pnuC"
                     /locus_tag="SI621_000214"
     CDS             complement(64843..65562)
                     /gene="pnuC"
                     /locus_tag="SI621_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEI4062983.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASMEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(65600..66643)
                     /gene="nadA"
                     /locus_tag="SI621_000215"
     CDS             complement(65600..66643)
                     /gene="nadA"
                     /locus_tag="SI621_000215"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEI4062984.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(66921..66996)
                     /locus_tag="SI621_000216"
     tRNA            complement(66921..66996)
                     /locus_tag="SI621_000216"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66961..66963),aa:Lys,seq:ttt)
     gene            complement(67030..67105)
                     /locus_tag="SI621_000217"
     tRNA            complement(67030..67105)
                     /locus_tag="SI621_000217"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67070..67072),aa:Lys,seq:ttt)
     gene            complement(67252..67327)
                     /locus_tag="SI621_000218"
     tRNA            complement(67252..67327)
                     /locus_tag="SI621_000218"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67292..67294),aa:Lys,seq:ttt)
     gene            complement(67331..67406)
                     /locus_tag="SI621_000219"
     tRNA            complement(67331..67406)
                     /locus_tag="SI621_000219"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67371..67373),aa:Val,seq:tac)
     gene            complement(67458..67533)
                     /locus_tag="SI621_000220"
     tRNA            complement(67458..67533)
                     /locus_tag="SI621_000220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67498..67500),aa:Lys,seq:ttt)
     gene            complement(67536..67611)
                     /locus_tag="SI621_000221"
     tRNA            complement(67536..67611)
                     /locus_tag="SI621_000221"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67576..67578),aa:Val,seq:tac)
     gene            complement(67647..67722)
                     /locus_tag="SI621_000222"
     tRNA            complement(67647..67722)
                     /locus_tag="SI621_000222"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67687..67689),aa:Lys,seq:ttt)
     gene            complement(67887..68678)
                     /gene="cpoB"
                     /locus_tag="SI621_000223"
     CDS             complement(67887..68678)
                     /gene="cpoB"
                     /locus_tag="SI621_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HEI4062985.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(68688..69209)
                     /gene="pal"
                     /locus_tag="SI621_000224"
     CDS             complement(68688..69209)
                     /gene="pal"
                     /locus_tag="SI621_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEI4062986.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(69244..70536)
                     /gene="tolB"
                     /locus_tag="SI621_000225"
     CDS             complement(69244..70536)
                     /gene="tolB"
                     /locus_tag="SI621_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEI4062987.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(70669..71967)
                     /gene="tolA"
                     /locus_tag="SI621_000226"
     CDS             complement(70669..71967)
                     /gene="tolA"
                     /locus_tag="SI621_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEI4062988.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAVADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAA
                     AEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAG
                     SGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPE
                     GGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(72032..72460)
                     /gene="tolR"
                     /locus_tag="SI621_000227"
     CDS             complement(72032..72460)
                     /gene="tolR"
                     /locus_tag="SI621_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HEI4062989.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(72464..73156)
                     /gene="tolQ"
                     /locus_tag="SI621_000228"
     CDS             complement(72464..73156)
                     /gene="tolQ"
                     /locus_tag="SI621_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HEI4062990.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(73153..73557)
                     /gene="ybgC"
                     /locus_tag="SI621_000229"
     CDS             complement(73153..73557)
                     /gene="ybgC"
                     /locus_tag="SI621_000229"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEI4062991.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(73707..74000)
                     /gene="ybgE"
                     /locus_tag="SI621_000230"
     CDS             complement(73707..74000)
                     /gene="ybgE"
                     /locus_tag="SI621_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HEI4062992.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(74000..74113)
                     /gene="cydX"
                     /locus_tag="SI621_000231"
     CDS             complement(74000..74113)
                     /gene="cydX"
                     /locus_tag="SI621_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEI4062993.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(74128..75267)
                     /gene="cydB"
                     /locus_tag="SI621_000232"
     CDS             complement(74128..75267)
                     /gene="cydB"
                     /locus_tag="SI621_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEI4062994.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(75283..76851)
                     /gene="cydA"
                     /locus_tag="SI621_000233"
     CDS             complement(75283..76851)
                     /gene="cydA"
                     /locus_tag="SI621_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEI4062995.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        18562 a        20515 c        19418 g        18428 t
ORIGIN      
        1 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
       61 ttctgcatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtctt
      121 tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag ccaataaata
      181 agaattgttc ttatcaatat aactaaccca ttgaattttt attagttttg tttttcacgc
      241 ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta
      301 tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg
      361 ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca
      421 gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt
      481 ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg
      541 aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg
      601 cagtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaactccgc
      661 tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagag ctggctgacc
      721 gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca
      781 ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt
      841 ctaacatcga ataaatccat cgttgatggt gcagaacttt agtacacgat aaaagcggct
      901 tcctgacagg aggccgtttt gttttgtagc ccgcctcaac gcacttattt agtgcatcca
      961 tctgctatcc ccagctggtt aagtaaattt tttgtatcca catcatcaca caatcgttac
     1021 ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt
     1081 ttagcgcacc agtttggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg
     1141 tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt gcaaaactgg cacgattttt
     1201 tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg
     1261 aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat
     1321 gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa
     1381 cagggcgata aatacgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagaactg
     1441 aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc
     1501 aaaaacgtcg atctggcgct ggcgggcatc accattaccg acgagcgtaa aaaagcgatc
     1561 gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac
     1621 gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggcactggc
     1681 tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatt
     1741 gataacgcct atatggaact gggcaccaac cgcgcagatg ccgttctgca cgatacgcca
     1801 aatattttgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct
     1861 ctggaagcac agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa
     1921 gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa
     1981 tggttcggta ctgaaccgaa ataataacgc tacgcctgta aagcgcactg gcagttccct
     2041 ctcccctctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta
     2101 ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt
     2161 gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg
     2221 gtcctcggtt tggctggcgg tctggtgatc ggattgctgg caggttttgc acgcaccttc
     2281 ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct
     2341 atcgtcgtcc aggtgatgtt tatttatttc gccctgccga tggcgtttaa cgacttacgc
     2401 atcgacccgt ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg
     2461 gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt ttcgtgaagc aggactggcg
     2521 ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt
     2581 atgctgccgc cgctgggtaa ccagtggatc atcagtataa aagatacctc gctgtttatt
     2641 gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc
     2701 gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctgagc
     2761 tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct
     2821 ccaagcattt tggtccaacc caggtgctgc acaatatcga tttgaacatt gctcagggcg
     2881 aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca
     2941 acaaactgga agaaatcact tccggcgatc tgattgtcga tggcctgaag gttaacgatc
     3001 cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc
     3061 tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc gtgcgtggcg
     3121 cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggcctggcag
     3181 aacgcgctca ccactaccct tccgagcttt ctggtggtca gcaacagcgt gtggcgattg
     3241 cccgtgcgct ggcggtaaaa ccgaaaatga tgctgttcga tgaaccgact tccgctcttg
     3301 acccggaact gcgccatgaa gtgctgaagg ttatgcagga cctggctgaa gaagggatga
     3361 cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cgcctgatct
     3421 ttatcgacaa aggccggatt gcggaagatg gcgatccgca ggtgttaatc aagaacccac
     3481 cgagccagcg cttgcaggaa tttttgcagc acgtctctta acttctcagc ctccctttcc
     3541 cccgaaaggg aggcacacca gattcctctc atttaaaatc gcccctcccc cagcatctat
     3601 acttatcttt ttgctctgtt ttctcactgg aggagtcatg cggtggatcc tgttcatcct
     3661 cttctgcctg ctgggcgcac ctgcccacgc ggtatccata cccggcgtta caaccacaac
     3721 gacaacggac tcaacgactg aaccggcccc ggaaccggat atcgaacaaa aaaaagcggc
     3781 ctatggcgca ctggcggatg tgctggataa tgacacctcg cgtaaagagt tgatcgacca
     3841 gttgcgcacc gtcgccgcga cgccccctgc ggaacccgta ccgaagatcg tgccgccgac
     3901 gctggttgaa gagcaaaccg tgctgcaaaa agtcaccgaa gtcagccgcc attatggtga
     3961 agccctttcc gcccgcttcg gacaacttta tcgcaatatc accggctccc cgcataagcc
     4021 gtttaatcca caaaccttca gcaatgcgtt gacccatttt tcaatgttag cggtattagt
     4081 gtttggtttt tactggctga ttcgtctgtg cgcactgccg ctgtatcgca aaatgggcca
     4141 gtgggcgcgg caaaaaaatc gcgaacgcag caactggttg cagcttccgg cgatgattat
     4201 cggggcgttt attatcgacc tgctgttact ggcactgaca ttatttgtcg gtcaggtttt
     4261 aagcgacaac ctgaatgcgg gcagtcgcac catcgctttc caacaaagtt tgtttctcag
     4321 cgcctttgcc ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt
     4381 ggcggaactg cgcccgttca cgatacagga cgagagcgcc cgttactgga gtcgccgcct
     4441 gagctggtta agcagtttga taggttatgg cctgattgtg gccgtgccga ttatctctaa
     4501 tcaggtgaat gtacaaattg gcgcgctggc gaacgtcatc attatgctgt gcatgaccat
     4561 ctgggcgttg tacctgatct ttcgtaataa aaaagagatt acccaacatt tgctcaactt
     4621 tgcggagcat tcgctggcct ttttcagctt gtttatccgc gcctttgcgc tggtgtggca
     4681 ctggctggca agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa
     4741 cagcctgaaa tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc
     4801 atttgtttcc ggtatgtttt cccgctggct ggcaaaaacc atcaccctct cgccacatac
     4861 tcagcgtaac tatccagaac tgcaaaaacg gttgaatggc tggctgtcgg cggcgctgaa
     4921 aacggcgcgt attctgacag tctgcgtggc ggtaatgttg ctgttgagcg catggggatt
     4981 gttcgatttc tggaactggc tgcaaaacgg cgcggggcag aaaactgtag atatcctgat
     5041 ccgtatcgca ctcattcttt tcttctcggc ggttggctgg acagtgctcg ccagtttgat
     5101 cgaaaaccgg ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac
     5161 cctgctgacg ctgtttcgta acgcgctggc ggtgattatc agtaccatca ccatcatgat
     5221 tgtgttgtcg gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gtgcattggg
     5281 gctggctatc tcttttggtt cgcaaacgct ggtgaaagat attatcaccg gggtatttat
     5341 tcagtttgaa aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac
     5401 tgtggaacgg atgtcgattc gctccgtggg cgtgcgtcag gataccgggg cgtatcacat
     5461 cattccgtgg tcttcgatta ccacctttgc taactttgtc cgcggcattg gttcggtagt
     5521 ggcaaattac gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga
     5581 tgcggtaacg gaattgatgg aaaacgaaga tattcgcggg ctgattattg gtgaaccgaa
     5641 ctttgccggg attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac
     5701 gctgccactc aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt
     5761 cgacctggcg ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc
     5821 gaccccggct gcaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa
     5881 acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc
     5941 ctgggccatc tcttttttaa ccaccttcac cgcgcccacg tccgtcagag cacgatacaa
     6001 cggcggtaag ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa
     6061 gcctttgctc tcttcaatca tctttttgat aaaggcgact tcaccgcctt cacaccacaa
     6121 ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg
     6181 tttacgctca ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa
     6241 ggtcgcgtcg tattgctcgt ttttatggat aatgccgtta aaaatcgccc cactctcttt
     6301 ttgccgacgc agacgaatgg cgcggttaag ccccggatta gcactgatga tcgcctgcac
     6361 actgcttaac gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac
     6421 gccaatcagc ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc
     6481 tggaattgtt ccgcttgctt cggccagtaa atcggcaagg tgatgaatat aatccgcccg
     6541 gcccggtact ggtgggcaga gaaaaccgtc cgggatatcc cagttcgcta cggcgtaaaa
     6601 atgggccagc aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct
     6661 ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag
     6721 cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc
     6781 ggacatagca ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa
     6841 tatctggctg cgtttatcat ttctaaaaac cttcacatgg ctatggttta tggcaattca
     6901 ccataaaaat catcatcatg catgttattg atttgtctta ttttgtaaaa atatatcgtt
     6961 caaaaaataa gcgcatctct gtcaattttt tgcgtcattt tcatcgcgca ggcgacctac
     7021 acttatctgg cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac
     7081 tactgattgc caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga
     7141 gtaacctgga cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa
     7201 acctaagcga tctggaggat aaactggcag aaaaagcgcg cgagcaaggc gcgaaaggtt
     7261 atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat
     7321 aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa
     7381 aaaacatcaa atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat
     7441 agaaaatcgc gccaagtgat aatgcttatc aaaattatta tcacttgcac gaacactatc
     7501 acgagattaa cagtggcatc gcatccgcag agaggctttc tcgtggcagt gaaaatttca
     7561 acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat
     7621 ttcattcgct cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac
     7681 tcgccgccga agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt
     7741 cgctttatgc gccacaacaa tctgcagatc cgaaattctc gcgtccggta gcggatacta
     7801 cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta
     7861 ccgacgcact caaaaacgtc cccggcgtgg gtgcgttttt tgcgggtgag aacggtaact
     7921 ccaccactgg cgacgccatt tatatgcgcg gtgctgatac ctctaacagt atttatattg
     7981 atggcattcg cgatatcggc agcgtctcgc gcgacacctt caataccgag caggtcgaag
     8041 tgattaaagg gccgtccggc accgactacg ggcgcagcgc accgacaggc tcgatcaata
     8101 tgatcagcaa gcagccgcgc aatgattccg gcattgacgc ctccgccagt attggtagcg
     8161 cctggttccg ccgcggcacg ctggacgtca atcaggtcat tggtgatacc accgcggcgc
     8221 gcctgaatgt gatgggcgaa aaaacgcacg atgccggacg cgacaaagtc aaaaatgagc
     8281 gttacggcat cgccccttct gtcgcttttg gccttggtac agcgaatcgt ttgtatctta
     8341 attatctgca tgtcacccag cacaacacgc cagacggcgg cattccgacc atcggtttgc
     8401 cgggctattc tgccccatct gcgggaacgg cggccctgaa tcattccgga aaagttgata
     8461 cgcataactt ttacggcacg gattccgatt acgacgattc gaccaccgac accgctacca
     8521 tgcgttttga gcacgacatc aacgataaca ccaccattcg caatactacc cgttggtcgc
     8581 gcgtgaagca ggattacctg atgacggcga ttatgggtgg ggcgtcgaat attacccagc
     8641 ccaccagcga tgtgaatagc tggacctggt cacgcacggc gaatactaaa gatgtgagta
     8701 ataaaattct caccaaccag accaacctga cctcgacgtt ctataccgct tctatcggtc
     8761 atgatgtcag taccggcgtg gaatttaccc gtgaaacgca gactaactac ggcgttaatc
     8821 cggtgacgtt acctgcggta aatatttatc atcctgacag cagcattcat cccggcggcc
     8881 tgacgcgcaa cggcgcaaac gccaatggtc agacggatac cttcgcaatt tacgccttcg
     8941 atacgctgca aatcacccgt gattttgagc tgaacggcgg gatccgtctg gataattatc
     9001 atactgaata tgacagtgcc accgcctgcg gcggcagcgg acgcggtgcc atcacctgcc
     9061 cagctggtgt ggcaaaaggt tctccggtca ccaccgtcga caccgccaag tcgggcaatc
     9121 tggtgaactg gaaagccggg gcgctgtatc acctgacgga aaacggtaat gtctatatta
     9181 actatgccgt ttcccagcag cctccgggcg gcaacaactt cgcccttgcg cagtctggca
     9241 gcggtaacag tgccaaccgc accgatttta aaccgcaaaa agccaacacc agcgagattg
     9301 gcaccaaatg gcaggttctg gataaacgcc tgttgctcac cgccgcgctg ttccgtactg
     9361 atatcgaaaa tgaagttgag caaaatgatg acggaactta ctcgcaatac ggtaagaaac
     9421 gcgtcgaagg ctatgagata tccgtggccg ggaatatcac tcccgcgtgg caggtgattg
     9481 gcggctatac ccagcaaaaa gcaaccatca aaaacggcaa agatgttgcc caggatggtt
     9541 cctcatcgct gccgtatacc ccggagcacg cctttacctt atggagccag tatcaggcaa
     9601 ccgatgatat ttccgttggt gcgggcgcac gctatatcgg cagtatgcat aaaggttctg
     9661 acggcgcggt gggaacgcca gcgtttaccg aaggttattg ggtcgccgac gctaaactgg
     9721 ggtatcgggt taatcgcaat ctcgacttcc agctaaacgt ctacaacctg tttgataccg
     9781 attacgtcgc ctcaatcaac aagagcggct accgttatca cccgggcgag ccaagaacct
     9841 tcttgctcac ggccaatatg catttctgat tcagatgtgg ggcgcaggcc ccactttttg
     9901 gagaacttgt atgatgtacc acattcccgg cgtgctatcg ccaaaggacg tcgcccgttt
     9961 tcgcgaacaa ctggaacaag ccgaatgggt ggatggacgc gtcaccaccg gcgcacaagg
    10021 tgcgcaagtt aagaacaatc aacaggtaga cacccgcagc acgttatacg ccgccctgca
    10081 aaatgaggtg ctgaacgcgg ttaaccaaca tgctttgttc tttgccgcgg ccttgccgcg
    10141 taccctttcc acgccgctgt ttaatcgcta tcagaacaat gaaacttatg gttttcatgt
    10201 ggatggcgca gtacgcagtc acccacaaaa cggctggatg cgcactgacc tttctgccac
    10261 gctgttttta agcgatccac aaagctacga tggcggcgaa ctggtcgtta atgacacctt
    10321 cgggcaacat cgggtaaaac ttccggcagg cgatctggtg ttgtatccct ccagcagctt
    10381 gcattgcgtg acacccgtaa cccgcggcgt acgagtggca tcatttatgt ggatccagtc
    10441 gatgatccgt gatgataaaa agcgcgccat gctgtttgaa ctggacaaca atattcagtc
    10501 gctgaaaagc cgctacggcg aaagtgaaga gatcctgtcg ctgctcaatc tttatcataa
    10561 tctgctgcgg gaatggtcgg agatctgacg cttgaactgc ccgagttcgt ccctatatta
    10621 gctacactta actgtacaag tattgatatg gggaggacga tatggcatcc ggttgggcta
    10681 acgatgacgc cgtcaacgaa cagatcaaca gtacaattga agatgcgatt gcccgcgctc
    10741 ggggtgaaat tccgcgtggc gaaagcctgt atgaatgtga agagtgcgga gcccccatcc
    10801 cgcaagcccg tcgggaagcc attcctggtg tgcgcttatg cattcattgt cagcaggaga
    10861 aagatttaca aaaaccagct tatacaggat ataatcgcag aggttcgaaa gacagccagt
    10921 tacgttaact acaactggcg ggatgtatag cgagtataat ttgtattttg cgtggcattc
    10981 cgtgccttta acgctgtcgt gactgccacg ctgcataaaa caagtccata agttcaacaa
    11041 ataaatggat tatcagtatg ttagatatca atcaatttat ttgaacaagg cggtcaattc
    11101 tcttcgattt tatctctcga aaaaaaacgt gatactcatc acatcgacga aacaacgtca
    11161 cttatacaaa aatcacctgc gagagattaa ttatgaaaac tatcaatact gttgttgctg
    11221 ctatggctct ttcaactctg tcatttggcg tgtttgccgc ggaaccggta acggcatccc
    11281 aggcacagaa catgaataaa atcggcgtgg tttctgccga tggcgcatcc accctcgatg
    11341 ccctggaagc gaaactggct gagaaagctg cggcagctgg ggcgagtgga tacagcatca
    11401 cttctgccac caacaacaat aaattaagcg gtactgcggt aatttataag taattcgtaa
    11461 gccggatcag gcgtttacgc tacgtccagc acaagtattt ccctgtctgt ttgccgacag
    11521 acgcatatgc tctaaccctc attgatccta tgttaccctt gtttgcccgt ccgccactgg
    11581 acgggctttt ttttagctgg ctaactgctg acagaaagcc tgcaacgtct cttccggcat
    11641 accaaccttt cgcgccgcat cacaaatggc ctgccagctt cccgcatcca atggaatccc
    11701 ctgctcctgc cgttcgcgac gcgtgttaac ttcccactcg cccggtagca aaatcggctt
    11761 atcgtcatca tgcggcgagg ctttcaccca ctcggcaaag gcttcggtct gcgcgctaca
    11821 atccggcgcg ccgaacagtt ccgggttgat gatgatagtg gtcatgcagt taagaatggc
    11881 atcgggactg gtttgtaacg ttttctgatg cgtcgtttta ccgccggaaa gcgccccacc
    11941 aagaatttca cacatcgctg caagggcgta gcctttatgt tcggcaaagg tcaacagcga
    12001 acccaacggc gactcctgca ttaccgccgg attggtcgtc ggcatgccgt taacgtcaat
    12061 caggcaacct ggcggcacgg ggacgccttt atgccaggcg acgcgggttt tgccaaatgc
    12121 gatggcgctg gtggcgtaat caagcaacag cgggaaatta tctttacgag ggaaaaccac
    12181 acagaacgga ttggtgccaa agcggctgtc gcgaccgtgg aacggcgcga ccattggaat
    12241 accgaccacg ctaacaaagt ggatagagac aaaccccgcc gctgcacact gctccgccca
    12301 gtaaccgata cggccgatat gatgcgagtt atggagcgcc acggcggcaa tgccgtgctg
    12361 atgcgctttc tcaatcccca gcgccatcgc ttcatgtgcc gcgacctgac caaatgcgcg
    12421 atcgccatcg agcgtgaccg ccgcccccgc ctctttaacg attttggcat gatggttaat
    12481 ttgcaggtgc ccctgactcc aggagcgcac atagcttggg atcatgccaa taccatgtga
    12541 atcatgccct gccaggtttg ccgcgattaa atgatcggca actaatttcg cttcttgttc
    12601 ctcactaccc atctgacgaa atacagcctg aataaaactg tgcagcgtct gagcatcaaa
    12661 gcgatgacca ctttccatgg ttaactcctg tttatgttat gtgtttgttg tttttttatg
    12721 ctgcacgccg ggcgcgggaa ggtcaataaa gatgcaggaa tttcatattg tggtggggta
    12781 aaagccggat gacatggctc atccggctga gatgaaaatt aaaatgcctg attaacacgc
    12841 gccaggccgt cgctgatagt tgccgcttcc cccgtagcca ccaggcagca agccatctgg
    12901 attttcagcg attcgggaat cggttcgctg ccagccaggc aacgctcaat ccactgcgcc
    12961 gtggtttccg gatcttttgc ttgtggcagt aactcgctac cagcagggtc ctgtttttca
    13021 tacagcaccc gcatcccttc acggtcaatg agattgatct gcgggcagcg ctgcggatta
    13081 gcatacactt cgccttcagt accatgcatt agtaacgccc gcccgccgat atcgctaaag
    13141 aacttcgcga ctcgcccgat gtattccgga tgcgaaacgc tggaaagacg cagtgcctca
    13201 ccttcggcaa atggcgtcgc cagtttcgcc agggtatgtg cactgttacg cacgcccatc
    13261 cgccagcgca tcgccagttg tttttccagc ggcgggcaaa acgcgccgac aggcataaac
    13321 accggttgat gttcgtcgag cttcgcctgc gcctgcccgc cgtgcaacgt tggcgtaata
    13381 cccatcagtt cgaaaatggt ttctgtcagc acgcgggttg gatcttcgct aaccccgtga
    13441 accaccacag gaaaaccgag tttgtgcagg agaatcgcca acaacggcgt caggttggcc
    13501 tgtttacgcg cgccgttgta actggggatg acaatcggca tcggcttgtc tgcaggcggc
    13561 gtcagcttga tggtgtgatt ttgcatggct tcgtaaaagc cgagcatctc tgcttcccct
    13621 tcccctttga tacgcagcgc aatcaatacg ccgcccaact caaggtcagg gacttcacca
    13681 ttgagcatat gggcatacag accgcgcgca gtatcccggt ctaaatcgcg cgcgtggttt
    13741 ttcccgcgcc cgatctcttt aatgattttg cgatagtcca tttacgactc cttacctgac
    13801 tcacatcatt aacgccgccg acggcgtggg gatttcgttt tttctttctt tttaactata
    13861 ccttcaggga cttccggttg ctctatcgga aataccggta atgcatccag tagccgcttg
    13921 ccgtagtttt tggtgagcaa gcgtttatcg taaatcacca cttcgcccca gcaaccgtgg
    13981 cttcgaatca gtcgcccaac ctgctgaatc aggttaaacg aggcgctcgg caggctttgc
    14041 acctcaaacg gatagcggtt gaggcttttc agccattccc cttcggtgat caccacgggg
    14101 ctgtcgatgg gcggaaaagc gattttgtgg atatgcacct ggctgagcag atcgcctttc
    14161 agatcaagcc cttcggcaaa tgactgtaag cccaccagca cgctgcgctc accgttgccg
    14221 acgcgtttgc ggtgcagttc aactaaacgg taacgcggct gatcgccctg caccagcaac
    14281 atcaggcgta aatccgtcac atagtcgaga aaccgctgca tcgcccgtcc gctggcaaac
    14341 agtaccaaca taccgagatg ttttttgctc tccacctgct cacggaaaaa ggctgccatt
    14401 tcagcaatgt gctgctcttc gttgtcgatg gaaggctcaa agcgcatccg gggaataaca
    14461 attttgccct gttcgcagtg gttaaagggc gaatccagcg ccacaaagcg gtcgcccgct
    14521 ttctctttca gaccactcat ctcctgcaaa cgcgaaaaac tgttcagcga acgtaaggtc
    14581 gcggaagtga caataatgtg cggaatactg cgccacagca gcctttccag ctgatcgctg
    14641 acacgaatac ccacacagtg aaaccagaga tgcagctgcc cttcgcgctc ttcccgcgtc
    14701 gcccatttgg tcaccggcgc accggaagat tgcgccagag acgccagccg ccagagtttg
    14761 ctttgcgcct cgaacatccc cagcgcgcga ttcatctgca aaatcaaccg atgcagacgt
    14821 acaatgtcat ggctgccggt tttctcactt aaatcgttaa gaaataactc cgccaggcca
    14881 cgcagcatct cggtgagttt tgccagccgc tggcagatct ccagcacttc atctggcagt
    14941 tcgcccatcg caaaacggtg ctctgcctcc tgcccggcag gcatgtagag attgagaatg
    15001 ttgtttaacg aggcgataag ctcatacaac tcttcacaat gcgcattcaa acgttcaggg
    15061 atcgccagcg gcgggatggt cttcgggcga aactgctcca tgcaggtagc gaccagtttc
    15121 gtgaacaagt ccaactgtag tcgataccac ggcgcggtga tttcggcact catctccagc
    15181 gcatcccgcg ccacatctgg caggtgatga ccttcgtcca gcaccagcag caaatttttc
    15241 gggtcaggca atacggcttc gctttccatc gccgccatca ccagcgcatg gtttgccacc
    15301 accacttccg cttcctgaat ctcccgacga gcgacaaaaa acgggcattc gcgatagtaa
    15361 taacagttgc ggttgaggca gctggctttg tcggtactta aacgacgcca gagatcgtca
    15421 tctatagcga tatcagtatg atcgcgcaga ccatcccatt tataagtgtc gagatcgccc
    15481 ttcagcttcg cacaacgttt ttgctcttcc tgattatttg gcgtcagttc gtcgtcaaga
    15541 aacgccagca gatcctgttg cgtgggttca gtgctggcga gcgccgtcag attacgcggg
    15601 caaacgtagc gcccacgccc aaaagcggca gtgaatttaa gatcgggaat gatctttttc
    15661 agcagcggta aatctttgct gtagatctga tcctgcaatg ccacgttggc ggtactcacc
    15721 accagcgttt tttgctcttc gcgggcaatg gcgatgccgg gaatcaaata agagagcgtt
    15781 ttcccaacgc cggtgggggc ttcaatcgcc agatgccgcc cttcttctcc ggccagcgtt
    15841 ttggcgacgt ccgcaatcat ctgccgctgc ggcgcacggg gaataaagtc ggggatctgt
    15901 tcctgaagcg ccttatacca ggcggcaatt tgcgctttaa gcgcggcggt taatgccatg
    15961 agaaaacctg aaatactgta taaacagcca atattgtggc atttttcggt tttcaggggt
    16021 aatggttttt ctgtttctgg aaagcggctc gcaataacgt tttccggatg tgaagttttc
    16081 tcgcggcaga agaaacatca ttgtgcagca aaagacattg tacatcagct tattccgctg
    16141 gcggccattg cagagcggta tgtaaaacac gcagtaccga gagcgtatcg ccctccactt
    16201 gatagacaag cacataatgc ggcctgacaa cggcttcatg tgtaccagcg actcgtccgg
    16261 cccggtacat ctccgggcgt tcacaggcct gttccgcaat ggcttcaaat tgattatcaa
    16321 gggctattgc cgctagcgga ttatctttac tgatgtattc catgatttgc tccctgtcag
    16381 acagggacat tggcttccat tccagacgca ttaatccccc ttcagcaagg cttttttacg
    16441 ggcagcaaat agacttttgg cctcttcatg agagagtgcc gggcgtggat cgtcaatggc
    16501 ggcctgcacc tgctctcgaa accaggcatc atacgcggca gcttcatgag ctcgttttaa
    16561 tgcctcagcc ctgtctggtc gggtcgtttt cttctgaccg tccgggtcgt aattagaggc
    16621 gtcgacttca aaccgggcaa taccaacatc tttcaggtag ttgacaaccg tttccagccg
    16681 tttgaacagg cggatttgcc gactgcgttg tgctgcaaga ggccgttcag tgtggccgta
    16741 tttcacggtc aacgaccagc ctccctgggt gccaataacg tgtgctgcat ccactacgcc
    16801 agattcaacc agtttatgta aggttgagtg atcgatcgtt tctggtgcca taatgcgcta
    16861 acctctttgt atacagcatg caatttataa ttaaagatag agcaattaat aataaattgc
    16921 agcaccttga tcgtaaattg cccaggataa aattgcggta gaaagagact tgttgcgaag
    16981 atgccgggca tcgcccggca taaaagatta ctgcgcagcg gcaggtttac gcggacggcg
    17041 gcgacgttgt tgttcgcctg ccggtttggc atcgccgaga cgacgagacg gtttttctgc
    17101 gggcttcgcg ttaccagact tcgcaccact ttccgcacgg cgtggttgct gttgaccgcg
    17161 accaccacct tgcccacgac cgccgccgcc acgttgctgg cgaccgttct ggatcggttc
    17221 ggctttgatt gacgggtccg gctcataacc cggaatcgca atgcgcggga tctctttttt
    17281 cagcagtttt tcgatatcac gcagcagttt gtgttcatca acacacacca gcgacaacgc
    17341 ttcaccggta gcagccgcac gaccggtacg cccgatacgg tggacataat cttccggtac
    17401 gtttggcagt tcatagttga ccacgtgcgg cagctcttca atatccaggc cgcgcgcagc
    17461 gatgtcagtt gccaccagta cacgaatatc gcccgattta aaatcagcca gcgcacgggt
    17521 acgcgcacct tgcgatttat tgccgtggat cgccgcacta cggatgccat ctttattgag
    17581 ctgttcagcc agatggttag cgccatgttt ggtacgggta aacaccagca cctgctgcca
    17641 gttccctttc ccaatcatgt gcgacaacaa ttcgcgtttg cgtttcttat caacaaagtg
    17701 aacatgctgg gtcacctgat cagacgcggt attgcggcgt gccacttcga tttccagcgg
    17761 gttgtgcaac agtttttccg ccagagcttt aatatcgtca gagaaggtcg cggagaacaa
    17821 caggttctgg cgcttcgcag gtagttttgt taacacgcga cggatatcgt ggataaagcc
    17881 catgtcgagc atgcggtcag cttcatcgag gacgaggatt tcaacctgat ccagcttcac
    17941 cgcattctga tgttccaggt ccagcagacg tcccggcgtt gcaaccagca catcaacgcc
    18001 gccgcgcaac ttcatcatct gcgggttaat gctgacaccg ccaaacacca ccagcgaacg
    18061 aatgttcagg tatttgctgt aatcacggac gttttcgcca atctgcgccg ccagttcacg
    18121 ggtcggggta agaatgagcg cacgtaccgg acgacgccct ttggcgtgcg gctggcgagt
    18181 gatcaggtgt tgcaacagcg gcagcgtaaa gcccgctgtt ttgccggtgc cagtctgggc
    18241 gctagccatc aggtcgcggc cttccagcac cgcagggatc gcctgctgct gaataggggt
    18301 gggttcacgg taaccctgct cggcaacggc gcgcaggata tcagggctta aacccaaaga
    18361 atcgaaagac ataactactc cgaaccgccc cgaccgttac cggtgtagtt ttcagggaga
    18421 tactgaaaag gaaaatgaca aaaaccacaa tgtcatgagg gagcggagtg tagcagtttt
    18481 tgtgacgcag cgcataaatt atccctgtga ctggcgttgc taaaattctt agccatactg
    18541 gacaactccc atatggagtc ataatcttaa tcaatcattt gattaagaat ggactgtgcg
    18601 atgaataatc ctgccatgac aatcaagggt gaacaggcga aaaaacagct gattgctgcc
    18661 gcactggcgc agtttggtga atatggaatg aacgccacca cccgcgagat agccgcccag
    18721 gccgggcaga atatcgctgc catcacctac tacttcggtt cgaaagagga tttgtacctc
    18781 gcctgcgccc agtggattgc cgattttatt ggcgagcagt tccgtccgca cgccgaagag
    18841 gccgaacgct tgttcgcaca accacagcca gatcgggctg ccatccgtga actgatcctt
    18901 cgcgcctgca ggaacatgat taagctgctc acccaggatg acactgtcaa cctcagcaaa
    18961 tttatctccc gtgagcagct ctctcccacg gcagcctatc acctggtgca cgaacaggtg
    19021 attagtccgc tacacagcca ccttacacgc ctgattgccg cctggaccgg ctgcgacgcc
    19081 aatgataccc gcatgatcct tcatacccat gcgttgattg gtgagattct ggcgtttcgt
    19141 cttggcaaag aaacaattct gttacgtacc ggctggaccg cgttcgatga agaaaagacc
    19201 gaactgatta accagacggt gacctgtcat atcgacctga ttttgcaagg attatcgcaa
    19261 aggagtttgt agtgatgaaa aaacctgtcg tgatcggact ggcggtagta gtacttgccg
    19321 ccgtggttgc cggaggctac tggtggtatc aaagccgcca ggataacggc ctgacgctgt
    19381 atggcaacgt ggatattcgt acggtgaatc ttagtttccg tgttgggggg cgcgttgagt
    19441 cgctggcagt ggacgaaggt gatgcgatca aagcgggcca ggtgctgggc gaactggatc
    19501 acaagccgta tgagattgcc ctgatgcagg cgaaagcggg cgtttcggtg gctcaggcgc
    19561 agtatgacct gatgcttgcc gggtatcgcg atgaagaaat cgctcaggcc gccgcagcgg
    19621 tgaaacaggc gcaagctgcc tatgactatg cgcagaactt ctataaccgc cagcaaggac
    19681 tgtggaaaag ccgcaccatt tcggcaaatg acctggaaaa tgcccgctcc tcgcgcgacc
    19741 aggcgcaggc aacgctgaaa tcggcacagg ataaattgcg tcagtaccgt tccggtaacc
    19801 gcgaacagga tatcgcccag gcaaaagcca gcctcgaaca ggcgcaggcg caactggcgc
    19861 aggcggagct gaatttgcag gactcaacgt tgatagcccc gtctgatggc acgctgttaa
    19921 cgcgcgcggt ggagccaggc acggttctca atgaaggtgg cacggtgttt accgtctcgc
    19981 taacacgtcc agtgtgggtg cgtgcctatg ttgacgaacg taatcttgac caggcccagc
    20041 cggggcgcaa agtgctgctt tataccgatg gtcgcccgga caagccgtat cacgggcaga
    20101 ttggtttcgt ttcaccgact gctgaattta ccccgaaaac cgtcgaaacg ccggatctgc
    20161 gtaccgacct cgtctatcgc ctgcgtattg tggtgaccga cgccgatgat gcgttacgcc
    20221 agggaatgcc agtgacggta caattcggtg acgaggcagg acatgaatga tgccgttatc
    20281 acgctgaacg gcctggaaaa acgctttccg ggcatgaaca agcccgccgt cgcgccgctc
    20341 gattgcacca ttcatgccgg ttatgtgacg gggctggtag ggccagacgg tgcgggtaaa
    20401 accacgctga tgcggatgct ggcgggatta ctgaaacccg acagcggcag tgccacggtg
    20461 attggctttg atccgatcaa aaatgacggc gcgctgcacg ccgtgctcgg ctatatgccg
    20521 cagaaatttg gtctgtatga agatctgacg gtgatggaga acctcaatct ttacgcggat
    20581 ttgcgcagcg tcaccggcga ggcacggaag cagacttttt ctcgcctgct ggagtttacg
    20641 tctcttggcc cgtttaccgg acgcctggcg ggcaagctct ccggtgggat gaaacaaaaa
    20701 ctcggcctgg cctgtaccct ggtgggcgaa ccgaaagtgt tgctgctcga tgaacccggc
    20761 gtcggcgttg accctatctc acggcgcgaa ctgtggcaga tggtgcatga gctggcgggc
    20821 gaagggatgt taattctctg gagcacctcg tatctcgacg aagccgagca gtgccgtgac
    20881 gtgttactga tgaacgaagg cgaattgttg taccagggag aaccgacggc cctgacacaa
    20941 accatggccg gacgcagctt tctgatgacc agcccgcacg agggcaaccg caaactgttg
    21001 caacgggcat tgaaactgcc gcaagtcagc gacggcatga ttcaggggaa atcggtacgt
    21061 ctgatcctca aaaaagaggc cacaccagac gatattcgcc atgccgacgg gatgccggaa
    21121 atcaacatca acgaaactac gccgcgtttt gaagatgcgt ttattgattt gctgggcggt
    21181 gccggaacct cggaatcgcc gctgggtgca atattgcata cggtagaagg cacacccggt
    21241 gagacggtga tcgaagcgaa agaactgacc aagaaatttg gtgattttgc cgccaccgat
    21301 cacgtcaact ttgccgttaa acgcggggag atttttggtt tgctggggcc aaacggcgcg
    21361 ggcaaatcga ccacctttaa gatgatgtgc ggtttgctgg taccgacctc tggccaggcg
    21421 ctggtgctgg gcatggatct gaaagagagt tccggtaaag cgcgccagca tctcggctat
    21481 atggcgcaaa aattttcgct ctacggcaac ctgacggtcg aacagaattt acgctttttc
    21541 tctggtgtgt atggcttacg cggtcgggcg cagaacgaaa aaatctcccg tatgagcgag
    21601 gcgttcggcc tgaaaagtat cgcctcccac gcgaccgatg aactgccatt aggttttaaa
    21661 cagcggctgg cactggcctg ctcgctgatg catgaaccgg acattctgtt tctcgacgaa
    21721 ccgacgtccg gcgttgatcc catcacccgc cgtgaatttt ggctacatat caacagtatg
    21781 gtagagaaag gcgtcacggt gatggtcacc acccacttta tggatgaagc ggaatattgc
    21841 gaccgcatcg gcctggtgta ccgcgggaaa ttaatcgcca gcggcacacc ggacgatttg
    21901 aaagcgcagt cggccaacga tgagcaaccc gatcccacta tggagcaagc ctttattcag
    21961 ttgatccacg actgggataa ggagcatagc aatgagtaat ccgatcctgt cctggcgtcg
    22021 cgtgcgggcg ctgtgcgtca aagagacacg gcagatcgtt cgcgatccga gtagctggct
    22081 gattgcggta gtgatccctc tgctgctgct gtttattttt ggctacggca ttaacctcga
    22141 ctccagcaag ctgcgggtcg ggatattgct ggaacagcgt agcgaagcgg cgctggattt
    22201 cacccacacc atgacaggtt cgccctacat cgacgccacc atcagcgata accgtcagga
    22261 actgatcgcc aaaatgcagg cggggaaaat tcgcggtctg gtggttattc cggtggattt
    22321 cgccgaacag atggagcgcg ccaacgccac cgcaccgatt caggtgatca ccgacggcag
    22381 tgagccgaat accgctaact ttgtgcaggg gtatgtcgaa gggatctggc agatctggca
    22441 aatgcagcga gcggaggaca acgggcagac ttttgaaccg cttattgatg tacaaacccg
    22501 ctactggttt aacccggcgg cgattagcca gcacttcatt atccccggtg cggtgaccat
    22561 catcatgacg gtcatcggcg cgattctcac ctcgctggtg gtggcgcgag aatgggaacg
    22621 cggcaccatg gaggctctgc tctctacgga gattacccgc acggaactgc tgctgtgtaa
    22681 gctcataccc tactatttcc tcgggatgct ggcgatgttg ctgtgtatgc tggtgtcggt
    22741 gtttattctc ggcgtgccgt atcgcgggtc gctgctgatt ctgtttttta tctccagcct
    22801 gtttttactc agtaccctgg ggatggggct gcttatttcc acgattaccc gcaaccagtt
    22861 caatgccgct caggtcgccc tgaacgcggc ttttctgccg tcgattatgc tttccggctt
    22921 tatttttcag atcgacagta tgcccgcggt gatccgcgcg gtgacgtaca ttattcccgc
    22981 acgttatttc gtcagcaccc tgcaaagcct gtttctcgcc gggaatattc cggtggtgct
    23041 ggtggtgaac gtactgtttt tgatcgcttc ggcggtgatg tttatcggcc tgacgtggct
    23101 gaaaaccaaa cgtcggctgg attagggaga agagcatgtt tcatcgctta tggacgttaa
    23161 tccgcaaaga gttgcagtcg ctgctgcgcg aaccgcaaac ccgtgcgatt cttattttac
    23221 ccgtgctgat tcaggtgatc ctgttcccgt tcgccgccac gctggaagtg actaacgcca
    23281 ccatcgccat ctacgatgaa gataacggcg agcattcggt ggagctgacc caacgttttg
    23341 cccgcgccag cgcctttact catgtgctgc tgctgaaaag cccacaggag atccgcccaa
    23401 ccatcgacac acaaaaggcg ttactactgg tgcgtttccc ggctgacttc tcgcgcaaac
    23461 tggatacctt ccagaccgcg ccgttgcagt tgatcctcga cgggcgtaac tccaacagtg
    23521 cgcaaattgc cgccaactac ctgcaacaga tcgtcaaaaa ttatcagcag gagctgctgg
    23581 aaggaaaacc gaaacctaac aacagcgagc tggtggtacg caactggtat aacccgaatc
    23641 tcgactacaa atggtttgtg gtgccgtcgc tgatcgccat gatcaccact atcggcgtaa
    23701 tgatcgtcac ttcactttct gttgcccgcg aacgtgaaca aggtacgctc gatcagctac
    23761 tggtttcgcc gctcaccacc tggcagatct tcatcggcaa ggccgtaccg gcattaattg
    23821 tcgccacctt ccaggccacc attgtgctgg cgattggtat ctgggcgtat caaatcccct
    23881 tcgccggatc gctggcgctg ttctacttta cgatggtgat ttacggttta tcgctggtgg
    23941 gattcggtct gttgatttca tcactctgct cgacacaaca gcaggcgttt atcggcgtgt
    24001 ttgtttttat gatgcccgcc attctccttt ccggttacgt ttctccggtg gaaaacatgc
    24061 cagtatggct gcaaaacctg acgtggatta accctattcg ccactttacg gacattacca
    24121 agcagattta tttgaaggat gcgagtctgg atattgtgtg gaatagtttg tggccgctac
    24181 tggtgataac ggccacgaca gggtcagcgg cgtacgcgat gtttagacgt aaggtgatgt
    24241 aacttcttat ctttcgccag caaagacacc accgccgggc cggcaaggat tgccagccct
    24301 gcaatcgcca gcaaaaagtt gttttccagt acacgggaca atagccacag cacaatcagc
    24361 gctgcgccaa aataccaggt cgtggtcagc tcttccagca cgtctgccac ctcacgccag
    24421 cgttgctcgg ggtggcgctg aaacaccagc atcatcacta ccgtgacaca atacagtgcg
    24481 cataatccca cgccgacgtg aaccagagtc ttactcatcc agcccaccac cagcagcgcc
    24541 actaccagta aatgcaacat aatctgccag caacttagac ccgttgcgac acgaacacgt
    24601 tgttgccact tcatggcttc tctcctgaag aatttatctc tacttactca gagtaccgaa
    24661 ctttacggaa aattccgtaa gaccgcacct ttttgtgacg ccgactacac tatttcaatg
    24721 agataagagg caaagacagg aaaattatgc ccgatcaaac acaacaattt tcgttcaagg
    24781 tgctcaccat caatattcac aaaggcttta ccgcgtttaa ccgacgcttc attttgccgg
    24841 aacttcgcga cgccgtgcgt accgtcagcg ccgatattgt ttgcctgcag gaagtgatgg
    24901 gcgcgcacga agttcatccg ctgcatgtgg aaaactggcc cgatacctcg cactacgagt
    24961 ttctcgccga cactatgtgg agcgattttg cctacggtcg caatgccgta tacccggaag
    25021 ggcatcacgg taacgccgta ctgtcgcgct atcccattga acattatgaa aaccgcgatg
    25081 tttcggtcga tggtgcggaa aagcgcggcg tgctctactg ccgcattgtg ccgccgatga
    25141 ccggaaaagc gattcatgta atgtgcgtac atttgggcct gcgtgaggcg caccgtcagg
    25201 cgcagcttgc gatgctcgcc gaatgggtga atgagctacc ggacggcgaa ccggtattgg
    25261 tggcgggtga tttcaatgac tggcggcaaa aagctaatca tccgttaaaa gtgcaggccg
    25321 gactggatga gatttttacc cgcgcccacg gacgcccggc gcgcacgttt ccggtgcaat
    25381 ttcctctact acgactggac aggatctacg tcaaaaatgc cagcgccagc gcgccaaccg
    25441 cgttgccgct gcggacatgg cgacaccttt ctgatcatgc ccctttaagt gcggagattc
    25501 atttatgaaa tgtagctggc gcgaaggcaa taagatccag ttgctggaaa acggcgagca
    25561 atattatccc gcggtgttta aggcgattgg cgaggcacaa gaacgcatca ttcttgaaac
    25621 gtttatctgg tttgaggatg acgtcggcaa acaactgcat gcggcactac tggcagcagc
    25681 gcaacgcggg gtaaaagcgg aagtcttact tgatggctac ggttcgccgg atctcagcga
    25741 tgagtttgtc aatgaactga cggccgctgg cgtggtgttc cgctactacg atccccgccc
    25801 tcgccttttt ggtatgcgca ccaatgtgtt tcgccggatg catcgcaaaa ttgtggtgat
    25861 cgacgcgcat atcgccttta ttggcgggct gaattactcc gccgagcata tgtccagcta
    25921 cggtccagag gctaaacagg attacgcggt gcgccttgaa gggccgattg tcgaagatat
    25981 tctgcaattt gaactggaaa acctgccagg acagagcgcg gcacgacgct ggtggcgacg
    26041 tcatcacaaa gcggaagaga accgccagcc gggagaagcg caggtattgc tggtctggcg
    26101 cgataacgaa gaacatcgcg atgatattga acgtcactat ctgaaaatgc tcactcaggc
    26161 acggcgggaa gtgattatcg ccaacgccta cttcttcccc ggctatcgat ttttacacgc
    26221 cttgcgtaaa gcggcacggc gcggggtgcg gatcaaactg atcattcagg gcgaaccgga
    26281 tatgccgatt gtcagagtcg gcgcgcgctt gctgtataac tatctggtta aaggcggcgt
    26341 tcaggttttt gagtatcgcc gccgcccgct ccacggcaaa gtggcgttga tggacgatca
    26401 ctgggcgacg gtagggtcca gtaatctcga tccgctcagt ttgtcactga atctcgaagc
    26461 aaatgtcatc atccacgatc gtcattttaa ccagacgctg cgcgataatc tgaacggcat
    26521 tatcgccgca gactgccagc aggtggatga aaccatgctg ccgaaacgca cctggtggaa
    26581 tctgaccaaa agcgtgctgg ccttccactt tttacgccac ttcccggcgc tggtcggctg
    26641 gcttccggca cacacaccac gtctggcgca ggttgacccg cccgctcagc cgacaatgga
    26701 aacgcaggat cgggtagaaa ctgaaaacac gggggtaaaa ccctgatgag taaatcacac
    26761 ccacgctggc gcttagcaaa gaagatcctc acctggctgt tttttatcgc ggtgatagtg
    26821 ttgctggtgg tctacgccaa aaaggtggac tgggaagagg tctggaaggt catccgcgac
    26881 tacaatcgcg ttgcgttgct cagtgcggtc gggctggtgg tcgtcagtta tctgatttac
    26941 ggctgctatg atctgctcgc ccgcttttac tgcggtcaca aactggcgaa gcgccaggtg
    27001 atgctggtgt cgtttatctg ctacgccttc aacctgacgc tcagtacctg ggtcggcggc
    27061 attggtatgc gctatcgttt gtactcccgg ctagggttac cgggcagcac tattacgcgg
    27121 attttctcgc tcagtattac caccaactgg ctgggctata ttttactggc agggattatc
    27181 tttaccgcag gcgtggtgga gttgccggat cactggtatg tcgatcaaac cacgctgcgt
    27241 attctcggca ttggcttatt gatgattatc gcggtttatt tgtggttttg cgctttcgcg
    27301 aagcaccgcc atatgaccat caaaggacaa aagctggtgc tgccttcatg gaaatttgcc
    27361 ctcgcccaga tgctgatttc cagcgttaac tggatggtaa tgggggcgat tatctggctg
    27421 ttacttggtc aaagcgtgaa ctatttcttt gtactgggcg tgttactggt tagtagtatt
    27481 gctggcgtca tcgtgcatat tccggcgggg attggtgtgc tggaagcggt atttatcgcg
    27541 ctactggctg gggagcatac ctcgaaaggg agcattatcg ccgccctgct cgcttatcgc
    27601 gtcctgtatt actttatccc gctgctgctg gcgctgatct gttacctgct actggaaagc
    27661 caggcgaaga agctacgagc gaaaaatgaa gcggcgatgt gaagtttagt taagttcttt
    27721 agtatgtgca tttacgacta atgaaaaaaa cgcgtatgcc tttgccagac aagcgttata
    27781 gctatcgcgg caagtatccc aaagaagccg ataaaatagt aatagagtgt taatgcacct
    27841 gtaaaagcgc aacgaaccgg actccacaac tcttcgctat ggcattttcc tgccagttca
    27901 aggagcttat ttcgtccatg cgagataatg cctgaccata acactatcat taagcaactt
    27961 attacccaaa cgggtcgttc actcattaat gcggcatcca tgatgatcac cagggccagc
    28021 tctgcggttg ccaccatacc caaagcggta atgagccata taccgtttaa tatcctggcc
    28081 tcgtgctgaa aatctaattt attgctgttt tgcgagtatg attccattct tttagttgtc
    28141 cttagaaaaa acattaatat tcaccatcaa taaatttaaa accacgcata caaaatattt
    28201 ttgtatttat ggatacggaa aacacttccg ctcggccata aggcatattt tatacctggt
    28261 acttgttaag tgtcgttttt agtaagaaaa aaatcccgac acatggccgg gatattgatg
    28321 gtgaaaaaga attaacggcg gttgccgaag atccgcaaca acatcaggaa caagttgatg
    28381 aagtccagat acaaggttaa cgcgccaaga atggaatatt tgcgcaggtt cgacgtgtcg
    28441 cgggtatcaa tctgctcacc catatttttc aatttctgag tgtcatacgc cgtcaatccg
    28501 acaaagacaa tcacgccgat gtaggtaact gcccacatca atgcttcgct tttcagccag
    28561 aagttgacca gcgatgccag cacaatgccg attaacgcca taaacagcat attgccaaag
    28621 ccgcttagat cgcgcttcgt ggtgtaaccg tacaggctca ttgcgccaaa catcccggca
    28681 gtaacgacga aagtactggc gatagaagca gcggtataga caatgaaaat actggaaagc
    28741 gtaagacccg tcagcgccga ataaagcata aagagcatcg tcgttacacc tgcgctcagc
    28801 ttttgaatca tcgctgataa cacaataacc aatgctaatt gcgcgatgat cagaccgatt
    28861 aaaaagacac ggttagtgaa caacagctcc atcacggccg cggaattagc cgcataccag
    28921 gcaacaaatg cggtcagcaa caagccaacg gtcatccagc catagacttg cgccatataa
    28981 gtttgcaagc cagcccgggg ttgtacaatt gaatcagaac gtgggaatct gtccatgacg
    29041 atctcctgaa gatataagga atatcttaag gatactgcaa aatgatgagg ctgtgcatcg
    29101 acgcagcgta aacgcatgta ctgagcggtg aaattgccgg acgcagcggt gccttatccg
    29161 gctaacaaaa aactaccagc gttttgccgc ctgctgatcg ctctcccgag cttcgaccca
    29221 gcggtcgcct tccggcgtgg cttcgcgctt ccagaacggt gcgcgggttt tgagataatc
    29281 cataataaac tgcccggctt caaacgcact gctgcgatgc gcactggtga caccgacaaa
    29341 aacgatttca tcgcccggcc ataattcccc gatgcggtga atcaccgtga cgcgccccag
    29401 cggccagcgg ttacgcgctt catcgacaat ttctgccagt gctttttcag tcatccccgg
    29461 atagtgttcg agggttaatg ctttgacgct gtcgccaagg ttatggttgc gcaccttacc
    29521 ggtaaaggtg actaccgcac cgtcttcatc acgctccgcc agccacggat actcttctcc
    29581 tacgctgaat ggctgcgggc cgacaacaat tttggtttct gccatcttaa cctccggtta
    29641 ccggcgggaa gaaagccact tcgtcgccgt cagtcagcgg atggtcaaga ctcaccagcg
    29701 tctggttgac ggcagccagc aatttgccat cttccagcgc cagcgcccag cgatcgctct
    29761 gcgcagccag atgttggcgt aacgcttcaa cggttgggaa atccgcagcc acttcggttg
    29821 catctgttcc caccaactcg cgcacctggg cgaaaaaaag aactttaatc atccgcttcc
    29881 accttaaagt cacccgactt gccgccgctc ttcgccagca aacgtaccgg accaattacc
    29941 atatcttttt gcaccgcttt gcacatgtca taaatggtca gcgccgccac ggaggccgcg
    30001 gttaacgctt ccatttcgac accggttttc ccggtcaggc ggcataaggt ttctatacgt
    30061 acccgattgt gctccggctc ggcctgtaaa ttgacttcaa ctttgctgag catcagcggg
    30121 tgacacagcg ggatcagatc ccaggtgcgt tttgccgcct gaataccggc gatacgcgca
    30181 gtggcaaata cgtcgccttt gtggtggcga ccatcaataa tcatcgccag cgtctcgctg
    30241 cgcatggtga caaaggcttc cgcccgcgct tcacgcacgg tttccgcttt ggcggagaca
    30301 tccaccatgt gtgcttcgcc agcggcgttg atatgggtca gttgcgacat acttatttct
    30361 tcaaatgtgg atggaaattg catggacgtg tacgggcatc gagctgcggc gcgatgatat
    30421 tttcccacgc ggtgcggcac gctttggtcg aacccggcat ggcgaaaatc agcgttttgt
    30481 tagcgacgcc cgctaccgca cgagattgca acgttgaggt gccaatctct tcaaacgaca
    30541 acatccggaa cacttcacca aaaccttcaa cttcacggtc gaacaacggc agcagcgctt
    30601 cgggagcctg atcaccttcc gtcaggccag taccacccgt aatcaatacc acttgtacat
    30661 cgtcgctggc gatccacgca gatacctgag cgcgaatagc gtagcggttt tctttcacaa
    30721 tggctttatc gacaacgtga tggcccgctt cctgcgccga atcgcgcaga tagtgaccgg
    30781 aggtatcgtc ttcttcacca cgacgattag aaaccgtaag aatagcaata cgggtcggga
    30841 taaattcagt gcttacctga ctcatctgat ctctcctttt gacgttttag ccgccaatgt
    30901 acgataagtt ttgcgtaata ccggtgttgt tttgatgcag gaaatgggtc tgtttcttct
    30961 cccgcagcgc cgctgaaata cgcgcttcca gcgcctgttg ctgggtatcg tcttccagca
    31021 gatcgcgcag gttaacgccg ccttcaccaa acaggcagag atggagttta ccaatggagg
    31081 aaacgcgcag gcggttgcaa gtggcgcaga agtctttttc atacggcatg ataaggccaa
    31141 tctctccggc gtaatccgga tgacaaaaga cttgcgcggg accgtcgctg cgttgacgta
    31201 attggtggat ccagccgcga cgcagtagct cgtcacgcag aacctgacca gagatgtgat
    31261 gcttacggaa gagctcgctg ccctcacccg tttccatcaa ttcgatgaaa cgcagctgga
    31321 tagggcgatg ctggatccag ttcagaaagg tgtcgagctg atgatgatta acatcacgca
    31381 tcagcacggt attgactttg accttttcaa aaccggcctc aaatgcggca tcgatccctg
    31441 ccatgacctg gttgaattta tcctgcccgg taatggcatg aaactggtga gcgtcaaggc
    31501 tatcaacgct gacgttgatg ccagtaagtc cagcatcgcg ccagttcgcc acatcgcgtt
    31561 ccagacggta accattggtg gtgaccgcaa tctggcggat agcgtcgttt tcccgcacag
    31621 cggcgatgat atcggtaaag tcgcggcgta aagacggctc accacctgtc agacggactt
    31681 tttcggtgcc aagactggcg aaggcgcgcg taacccggcg aatttcatcg acggtaagaa
    31741 agcctttatt ggtgacgccg ctcggtttgt agccatccgg caggcagtag gtgcaacgaa
    31801 agttacacac atcggtaatc gacaggcgca agtagtaaaa cttacgcgca aatgcatcag
    31861 tcagttgtga agccatgtac acctttccag atacgggagg cgaagtcatt tcttccttcg
    31921 ccctggtggc aatctttacg agcattgtca cggccaaagc accgtatcag ttgacccagg
    31981 tgcagaggct agagtgttta gtggttaagc cgatactagc gtgtaaatga gatttttacc
    32041 atccacactt tcgctatgta atcatgtata tagcgtcatg atcgtgctat ttcgctacag
    32101 aataagggaa aatggcggtt ttactttgat atacatcatc aggaaggcat agcgtacatc
    32161 gtcctttaga ggtagaagaa aatagtttgt atcctcaata ttggcaggtt aattgctgtt
    32221 tccccgcaat ttgcgctact gtagcgcgga taatctgact ccaggaaact atatgcgcaa
    32281 tcgtacgctg gctgaccttg atcgtgtcgt tgctctcggc ggagggcatg gactgggacg
    32341 cgttctctca tcactttcgt ctttgggttc tcgtttaacg ggtatcgtca ccaccacgga
    32401 taatggtggc tcgacggggc gtattcgccg ctcagaaggc ggtattgcct ggggcgatat
    32461 gcgtaactgc ctcaaccagc tgataactga acccagcgtc gcttccgcaa tgtttgaata
    32521 tcgtttcggc ggtaatggcg aactttccgg acacaacctt ggaaacctga tgctgaaggc
    32581 actggatcac cttagcgtgc gacctcttga agcgatcaat ctgattcgca atctgctgaa
    32641 agtggacgca catttgattc cgatgtcgga gcatcctgtt gatctgatgg cgattgacga
    32701 tcaggggcat gaagtttacg gcgaggtcaa tatcgaccag ttaactgcgc cgattcaaga
    32761 gttattgtta acgccgaatg tacccgcaac ccgtgaggcg gttcacgcta tcagtgaagc
    32821 ggatctcatc attattgggc ctggcagttt ttacaccagc ctgatgccaa ttctgctgct
    32881 gaatgaaatc gcacaagcat tacgccgcac gccagcgccg atggtctata tcggcaatct
    32941 ggggcgtgag ttaagtttac ctgcggctaa tctgaagctg gaaagcaagc tggcaattat
    33001 ggagcagtat gttggtaaaa aggtcattga tgcggtcatc gtcgggccca aagtggatgt
    33061 ctcggcggtg aaagagcgga ttgtgattca ggaggtgctg gaggccagcg atattccgta
    33121 tcgtcatgac cgccagttgc tgcatagcgc gctggaaaag gcattacagg cactgggtta
    33181 atgtggccga gattgctgac caggcttctg gtgcaatgcg acctgttccc ctcaccctaa
    33241 ccctctctcc ggaggggcga ggggaccgtt cgagcacttt tttgacttga tcgttaatct
    33301 gagtcggaag tacattccgg ctttttcatc ttgtcacgac gcggcaataa acagctcacg
    33361 cagctgatgc aactggtcac gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg
    33421 ttgcatcatc aacccttcca gttcatggat cttctgctgc aacgctttag gcgacatatc
    33481 catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct tcgctttggt
    33541 tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca agccttgcgg
    33601 cgtaatgccg tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc
    33661 aatcgctttc gccattgatg gggtgatctt atcgccgtag agaatcgctt taccgttaac
    33721 gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc
    33781 tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac cttcgcgcag
    33841 taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat
    33901 acgttcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag
    33961 atattcagtg agatcttccg ccatccgctt ggtcagagtt gtaaccagta cgcgttcgtt
    34021 aattgccgct cgctgacgaa tctccgaaag aagatcatcc acctgtgttg ctaccggccg
    34081 cacttcgata atcgggtcga gtaagcctgt tggacgcacc acctgatcca ccacatcacc
    34141 accggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc
    34201 taatgcttcg aactcttcaa atttcagcgg acggttatcc agcgctgatg gcaggcggaa
    34261 gccgtactcc accagagtct ctttacgcgc ccggtcaccg cgatacatgc caccaatttg
    34321 tggaatggtg acgtgagatt cgtcgacgac cagcagccca tcggcaggca ggtaatcaaa
    34381 cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat
    34441 ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag
    34501 ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg cggccagttc
    34561 ttctttgatc tcctccatcg cctgtacgat gcgctcacgt ggcgtgacgt agtgcgtttt
    34621 cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggttagcg ggtcaaataa
    34681 cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc
    34741 tgccgggaag atatctatca cctcaccacg aacgcggaaa gtaccgcgct ggaatgcctg
    34801 atcattacga gcgtattgca gctccgccag tcggcgcagg atcgcgcgct gatcgataat
    34861 catgccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata
    34921 aatcgcggaa acagacgcca ccacaaccac atcacgccgc tccagcatcg ctttggtagc
    34981 ggacaaacgc atctgctcaa tatgttcgtt taccgaggca tctttctcaa tgaaggtgtc
    35041 ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga aatattccac
    35101 cgcgttttcc gggaagaact ctttcatttc gccgtacagt tgggccgcca gcgttttgtt
    35161 gggcgccagt accatggttg ggcgctgaag gtcagcaatg acattggcga tggtgaaggt
    35221 tttcccggag ccagttacac cgagtaacgt ctggtgcgcc aggccatctt ccagcccctc
    35281 ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt
    35341 gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac
    35401 tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttt ctcaccataa
    35461 tatttctgtt acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag
    35521 ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag
    35581 atgagtgatg acagttttat ggcaagagat gcctcttcag tgactgccat tgcaattatc
    35641 taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca
    35701 agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca
    35761 ggaatagtgg ataactgtct gcagccccta tcccccgccg cctgggcaat tcccacattc
    35821 accgcatgat gcggaggtaa aatttttttc gtgatttttt tagaatttaa ttggttaaat
    35881 tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aagtacagga tgactacatt
    35941 aaggcaagat tgatgtactt tccggttgcc gcattttctg gattttctgc aagccaggga
    36001 atttcgccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata ttcagcgtga
    36061 cgttttcccg gaggcgtgac atcgttcgcc acccaacccg ccagagtcag tccggcgtgt
    36121 tgtattgcct gtgccgtcaa catcgcgtga ttaatacagc cgagtttcac accaactacc
    36181 agtatcaccg gcagttgttc ctgtgttacc caatccgcaa aagtgaaagt gtcagaaagc
    36241 ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg ttcaagcgcg
    36301 cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgtgc gctgatgatg
    36361 tgcggcgaag tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc cagctgcagg
    36421 ctgctgttgc gctgtaacgc cagcgcgtcg ctattacgta agccttccgg ggtcttttcg
    36481 ctgccagagg cgaccggttt ataacctgcc gtccggtagc ctactgcctt tgcggcttgt
    36541 aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt gacaaaataa
    36601 cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat atcgcccctg
    36661 tttttgtggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata tccgcgggtc
    36721 gcgcccttca tgaagatgcg tggcaccgat gcctttcagc gaacgcatgg cactgagcgc
    36781 atcatcaaac cacagcgtga tgggctgaat atgatgttga taatgcacgc cgttcagtga
    36841 ctgttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt ccaccgcctg
    36901 ccacgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg cgaccacgcc
    36961 tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac cgcaccactg
    37021 caccgcgaga ttgctccatg caagatcgaa ggttgcagtc gctaacggca gggattcgat
    37081 atctcccgcc agataatggt ctgcggcatc cttctggcgt gcctgaacaa gcattggcgg
    37141 cgagagatct aacgccgtca cctgcgcgtg acgttcccgc caatgacggc tcatccagcc
    37201 aggtccacaa cccgcatcca gtacgtgggt gtatttacgc tgtgggagca ttgccagtaa
    37261 gacgtcagca ctctggcgct gtagatccgc atgttgctca tagtgcgcgg ctgcccgacc
    37321 aaatgccgct gcaatggctt gtttattaac cgttgtcatg cagcacctcc agcagactat
    37381 cgatatcctg catttcatgc gctgcggtta gcgttaagcg cagtcgcgca gtaccagcgg
    37441 gtacggttgg cgggcgaatc gccgtgaccc agcaaccttg ctggcgcagt ttttctgcca
    37501 gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctc catgaatcag
    37561 caagcgtaaa cggcaaatcc tgcactccgg cacgaaaacg cgtaatgagt gccgccagtt
    37621 tttcgcgccg tgcatcaccc tcatcactgc gaatgaccgc cagcgacgca cgtaatgcct
    37681 gcgcctgagc gggcggcata ctggtgctgt agataagatg gcgggcgaat tgcagcagat
    37741 aatccgccac cgtattggag caaagcaccg ctgccccgct gacgccaaat cctttgccaa
    37801 aagtcaccac cagcaattct ggttttacct tttgcagcca gcagctgcca cgcccctgct
    37861 ccccgataac gcccgtgccg tgggcgtcat cgaccatcaa ccagccatcg tgctgttgcg
    37921 ttacctgctg gatttcctcc agtggcgcac tatcgccgtc catgctgaac acgccttctg
    37981 tcactaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga tgagtgacat
    38041 cgttatgagc aaaacggcga agcggcgacg ggcttaaact tgcagcctcc agcaatgagg
    38101 catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg gtaataactg
    38161 cctggttagc ggcaaaacca gagataaaca gcagtgcccg cgaatagccc agccactcgg
    38221 ccagttcttc ttccagcgcc tgatgcgcca cgctataacc gctgacgtga ccggagccgc
    38281 cgctaccgac gccaaattgc tccgcactca gcttccaggc acggataatt tgcggatgat
    38341 ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatcg tccgccacca
    38401 gccagcgtcc ggctccttgc gccactggat aacggcgacg cagggcatcg gcagcacgcc
    38461 gcgcatcgag cgccgcgttg attttctcct gccagctcat agtgctgccg cgttgtaata
    38521 ttcgtcagtg tccggggtca tcagcgcctg ctccagacgc tgctgttgtt cgttatcgcc
    38581 cgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt gcaggtcttt
    38641 atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg agtttgcgcc
    38701 agccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg cagaaaggcg
    38761 tacgtaagag gtcggcatca tgatccgcgc aactgcaatg gtgcgaataa aatcaaaggc
    38821 atcgacatca tcgttatcag caagcggcgt gcctttcacc ttcaccagca tgttgattgg
    38881 tacgctttcc ggcggcgttg gcaggtttgc cagttgcagc aataatccgg cgcgatcttt
    38941 tactgtttcg cctaagccca caatgccgcc cgagcagact ttgatcccgg cgtcgcgcac
    39001 tttttccagc gtatcgaggc gttcctgata ggtacgcgtg gtgatgatat tgccgtaaaa
    39061 ctccggcgag gtgtcgaggt tgtggttgta gtaatccagc ccggcgttcg caaggcgctg
    39121 cgcctgagat tcactcaacg tacccagcgt catacacgcc tccagcccca tcgcttttac
    39181 tccctgcacc atttgttcca ggtagggcat atcgcgttcg tggggattct tccacgccgc
    39241 gcccatgcag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact ccagcacctg
    39301 ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc tttgcgggca
    39361 gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaaagtgc tgacctgcac
    39421 ctgacgtgga tcgaaatgct gacgatgcac ctgctgcgct tcaaacagca gatccagcaa
    39481 cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt gagccatagg
    39541 gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag actcgtaaac ctaaatcttt
    39601 tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac caacgccata
    39661 tctggcaccc atacacatcc atgacctccc cgctaccggt ttatccggtg gcgagcgccg
    39721 agggttgcga gctaattttg tctgacggca gacgccttgt tgacggtatg tcgtcctggt
    39781 gggcggcgat ccacggttac aatcacccgc agcttaatgc ggcgatgaaa tcgcaaattg
    39841 atgctatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatt gagctgtgcc
    39901 gcaaattggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg gactccggct
    39961 ccgtagcggt agaagtggcg atgaaaatgg cgttgcagta ctggcaggcc aaaggcgaag
    40021 cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt ggcgcgatgt
    40081 cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg ccagaaaact
    40141 tgtttgctcc cgccccgcaa agccgcctgg atggtgaatg ggatgaacgc gatatggtgg
    40201 gctttgcccg cctgatggcg gcgcatcgtc atgaaatcgc cgcagtgatc attgaaccga
    40261 ttgtccaggg cgcaggcggg atgcgcatct accatccgga atggttaaaa cgaatccgca
    40321 aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc ggatttggtc
    40381 gtaccggcaa actgtttgcc tgtgaacatg cagaaatcgc gccggatatt ttgtgtctcg
    40441 gtaaagcctt aaccggcggc acaatgaccc tttccgccac gctcaccacg cgcgaggttg
    40501 cggaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact tttatgggca
    40561 atccgctggc ctgcgcagca gcaaacgcca gcctggcgat tctcgaatct ggcgactggc
    40621 agcatcaggt ggcggctatt gaagggcagc tacgcgagca acttgccccc gcctgtgacg
    40681 ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg cgtggtcgaa accactcgtc
    40741 cggtgaatat ggcagcgctg caaaaattct ttgtcgaaca gggtgtctgg atccggcctt
    40801 ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag ttgcagcgtc
    40861 tgaccgcagc ggttaatcgt gcggtgcagg atgaaacatt tttttgccaa taacgggaag
    40921 ttagcgtgag ggtttctggc tacactttct gcaaacaaga aaggagggct catgaaactc
    40981 atcagtaatg atttacgcga tggcgataag ctgccgcatc gtcatgtctt taacggcatg
    41041 ggttacgatg gcgataatat ttcaccgcat ctggcgtggg atgatgttcc tgtgggaacg
    41101 aaaagttttg ttgtcacctg ctacgacccg gatgcgccaa ctggctccgg ctggtggcac
    41161 tgggtagttg ttaatttacc cgcggatacc cgcgtattac cgcaagggtt tggctctggt
    41221 ctggtagcaa tgccagacgg cgttttgcag acgcgtaccg actttggtaa aaccggatac
    41281 gatggcgcag cgccgccgaa aggcgaaacc catcgctaca tttttaccgt tcacgcgctg
    41341 gatgtagaac gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg gtttaacgtt
    41401 cattttcact ctctggcgag agcctcgatt actgcgatgt ttagttaatc actctgccag
    41461 atggcgcaat gccatctggt atcacttaaa ggtattaaaa acaacttttt gtctttttac
    41521 cttcccgttt cgctcaagtt agtataaaaa agcaggcttc aacggattca tttttctatt
    41581 tcatagcccg gagcaacctg tgaacacatt ttcagtttcc cgtctggcgc tggcattggc
    41641 ttttggcgtg acgctgaccg cctgtagctc aacaccgccc gatcaacgtc cttctgatca
    41701 aaccgcgcct ggtacctctt ctcgcccgat tctgtcggca aaagaagcgc agaatttcga
    41761 tgctcaacac tattttgcat ccctgacacc aggtgctgca gcgtggaatc cttccccgat
    41821 taccctgcct gcacaacctg actttgttgt cggcccggcg ggtactcaag gtgtaacgca
    41881 taccacgatt caggcggcgg tagatgcggc aattatcaag cgtaccaaca agcgccagta
    41941 tattgccgtg atgcctggtg agtatcaggg aacggtgtat gtccctgccg ctccgggtgg
    42001 aattactctg tacggtacag gtgaaaaacc gattgatgtg aaaattgggc tttcccttga
    42061 tgggggcatg agcccagccg actggcgtca cgacgtcaac ccacgcggca aatatatgcc
    42121 gggtaaacca gcgtggtata tgtacgatag ctgccagagc aaacgcagcg acagcattgg
    42181 cgtgctctgt tctgcggtct tctggtcaca aaacaatggc ctgcaactgc aaaacctgac
    42241 catcgaaaac acactgggcg atagcgtaga tgcgggtaac catccggcgg tggcactgcg
    42301 tactgatggc gacaaagtgc agatcaataa cgtcaatatt ctcggtcgcc agaatacctt
    42361 ctttgtcacc aacagcggtg tgcaaaaccg tctggaaagc aaccgtcagc cgcgtaccct
    42421 ggtgactaac agctacattg aaggggatgt ggatatcgtt tctggtcgcg gcgcagtggt
    42481 gttcgataac accgaattcc gcgtggtgaa ctcccgtacc cagcaagaag cgtatgtgtt
    42541 tgcaccggct acgctgtcca acatttacta cggtttcctc gccgtaaaca gccgtttcaa
    42601 tgcttccggg gatggcgtgg cgcaactggg ccgctcgctg gatgttgatg ccaataccaa
    42661 cggtcaggta gtgatccgtg atagcgccat caacgaaggt tttaacacgg cgaaaccgtg
    42721 ggccgatgcg gtgatttcca atcgtccgtt tgcgggtaat actggcaacg ttgatgataa
    42781 cgacgaagta cagcgcaatc tgaatgacac taactacaac cgcatgtggg aatacaataa
    42841 ccgcggcgtg ggtagcaaag tggttgcaga ggcgaagaag taaaaaagaa aacccgatag
    42901 tctgaaccat cgggttaatg aaactgggcg ctatatttca tagcgccttt ttttacatct
    42961 gacggttttt attgaagtta atcaaactac ccgccttgat aatctcgcgc tcttcggccg
    43021 tcaggctttc catgtaaagc gtaatttccg ttaccggcgc atcttcatgg atcacataac
    43081 ctttaaacgt cgtacccgga ttatccagcg ctgctttaat gccagggatg taaatgtaat
    43141 cacctacgtc aaagttcggt gcttccgcca tctgcagcgg taacatcccc cagttgatga
    43201 cgttagaacg atagcgttta gtcgcgtact cctcggcaat attcgccaga ccgccaatca
    43261 cacgctgaca actcgccgcc tgttcacgcg cagacccatc gcctggtttc accgcataaa
    43321 ccatgctgcc aatttcagtt tgcagcggat caatatgctc ctgaccagca atctgcttaa
    43381 tgcgcgcaaa cacctctgtc agctcgctga catttcccgc cagacgctga ttttccagct
    43441 cagcagtcgc tttacttcta ccaacataac ctggatcgcg gcgtgacagg gtaaactccg
    43501 ccagaccaat cggatttgaa cgataagaag aggtttcacc ggaaggaatc agttcgtcgg
    43561 tggtggtcac ttcgtcgagg atcttcgagc acactttgag gacgatattg tcagtcagcg
    43621 cacccaattc tggccagtct ttaatgttcg gtccgtaaat cagcggttgc tgggtcgccc
    43681 ctttcacaaa gccctgataa acacggtttt tatacggcgt tacatcgaag gcgtactccg
    43741 gcacgttgtc ccagcaatcc agctcgctgg cagaggttaa atagccaccg tttgccgcag
    43801 tcgccgcaat agaacgagcg tccatcaacg ccaccgctga catctgccca ttagctggct
    43861 tagagccttc gcggttcggg aagttacgcg tggtgtggcg aatactcaaa ccgttgttga
    43921 ttggcgtatc gcccgcgcca aagcatgggc cgcagaacgc ggttctgatg attgagcctg
    43981 cgccaatcaa atctgcgacc acaccttttt tggcgagatc cataaacacc ggctgtgatg
    44041 acgggtaaac cgccagcgag aaggtgtcat tgccacagga ttgaccgcgc agtgcattcg
    44101 ccgccgcgat gacgttttcg tagttaccgc cagaacagcc cgcgataatc ccctgctgca
    44161 ctttcaggcg accgttttcg actttatcca gcagcgagag tttggcttta ccgtgcgcca
    44221 cgcgttcgga ctcaatttca atctcacgca gaatgtcggt caggttctgg ttcagcgtgt
    44281 cgatttcata gacgttgctc gggtggaacg gcagcgcaat cattggtttg atggcgctta
    44341 aatcaacgct gatgcagcca tcgtagtacg ccattggttg agggttaagc tggcagtaat
    44401 cctggccgcg accgtgcaac gccagccagt tatggacttc ttcatcggtt tgccagacag
    44461 aacttaaaca ggtcgtttca gtggtcatca cgtcaacgct gttgcggaaa tcggtagaga
    44521 gcgctgcaac accgggacct acgaactcca tcactttgtt tttgacgtaa ccgtttttga
    44581 acacggcacc gatgatagcc agcgccacat cctgcggccc cacatacggc gcaggttttc
    44641 cggtcagatg caccgcaacc acgcccggat agtcgatatc ccaggtgtca ttaagcagtt
    44701 gttttaccaa ctcgccgccg ccctcgccaa ccgccattgt ccctaacgca ccgtaacggg
    44761 tatggctgtc tgacccgagg atcattttgc cgccgcctgc catcatctca cgcatatatt
    44821 gatggatgac cgcaatgtgc ggaggcacaa aaataccgcc ataacgctgg gctgccgata
    44881 aaccaaaaac atgatcatca ccgttaatag tgccgccgac ggcgcagagt gagttatggc
    44941 agttggtcag cacatacggc agcgggaaac gttccatacc ggacgcttta gcggtctgta
    45001 caataccaac aaaggtaata tcgtgagagg ctaatgaatc aaacttaatt ttaagtttat
    45061 ccatatttcc ggacgtatta tgagaagaga gaatagacca ggcaatagtg ccttttttgg
    45121 cttcttcttt tttaatttcg ccggtgaaat gttcttcggc aattatttcg ttattactgg
    45181 cgagaaacac gcctttttca gataacttga tcatcaaatg ctccagatat ctgcgcaccg
    45241 gaaagtgcgc agaaagataa taaatggata tactctaaat aattcgagtt gcaggaaggc
    45301 gacaagagag tgaatcccca ggcgcttaca taagtaagtg actggggtga gcgaacgcag
    45361 acgcagcaca tgcaacttga agtatgacga gtatatttac agcatgttcc agccgatcag
    45421 catattccac caccacacgc cgagggtgat atgcaccagg aaggttaata tcgtcagtac
    45481 cgcaccgacc aaccaccagg atttaatatc gttgtaaccc acgccaaaga tgaccggacc
    45541 tgccgcgccg ccatagtgag taaccatgcc gccataggag ttagagaaca acagtgccag
    45601 cgcggttaac attaacggtg cgccggagac attcgccagc atggcaaata ccggtaacat
    45661 ggcaacgata taggcactac cagaagcgaa gaaataacgc acgatgatgc tgaggaaaat
    45721 aataacgaag aaagcaacgt tcccgtgacc atcaaatgcc aggttatttt taaagacttc
    45781 agctaaccat tcgaagaatt taactttcga taataaggag cttaagccga taataccgcc
    45841 gtaccagatt aaggtattcc agccgccttt atttttaacc acgtcttccc aggtaacgat
    45901 acccagcagc agcatagttg ccataacaac gattgccacg gtggattcat caccccccag
    45961 agacttactg aaaatccagc ccagcagcgc cagcacaaag acaccgagca gcattttttc
    46021 gcggattttc atcggtccca gttcggcaag gcccgcttta gcgatggttt tgttatccac
    46081 cttcttaatt tctggtggat acatggtgta aatcaccagc ggggtgacca gcagcataat
    46141 gatgccaggc aatccggcgg ctagcgccca tccaccccag ctaatttgca ggtgcagaat
    46201 gtcgttgatc attttcagcg ccagaatgtt ccccgccatt gcggtaaaga acatatagct
    46261 ggtggttttg gtgaccatgt aaatggacat catcaggtaa tgtccgacac gacgcggact
    46321 tttttccggt tctgacccca aagccaccgc cacgctgttg atgatcggta acacaatgcc
    46381 gcccgcacgc gcggtgttag acggtgttgc cggagccagt accagatcga gaaataccgt
    46441 aacgtaaccc agacccaggg tagtgctacc aattttacca atcagcagat aggcaatacg
    46501 tttacctaag cctgtggtta caaacgcggc gcttaaagta aacgcagaaa acaccagcca
    46561 ggtggtaccg gaagagtaac cgcttaatac ggcggtggtt ttaaattccc cacctgataa
    46621 gttacccacc actaccatcg atgcggcgac ggcaattaac agtacgacag gttccgggaa
    46681 aggcttgata accagcccca caatggccgc caggtaaata ccaaaaagca cccacgccag
    46741 ttcgcttaat cctgctggag ctggcataaa gccaataata catgggattg ctaatatcag
    46801 aattagcttc cataacgatt tcttattcat ttaatatcct gttctttctg tatgactcat
    46861 acagaagtcc tttattgatt gtccttatcc tgacaacgaa ttttttcagg gaagataaac
    46921 ttcaccggaa aatatttttc tggtcgtccg aataacagat gcgcgtaacg tcgtggcatc
    46981 ctgaccttca ttacttaaat gaacgtcgag cgcaccactg gggtgttcaa tattgatatt
    47041 gccgtatcct acagaaggga cgatttgtcg ggtgacggtg ccttccaatg cacaactact
    47101 ggaaatagca atagcaccgg ttatcgccaa agcgcgatgg caagaatgcg gcataaaata
    47161 acgcacatta attgccccgc ctttctgcgc tggagaaata agtacaggtt tagggataac
    47221 catattactg acatcgccta agcccattgc tttacccgct tgtagacgga tagattcaat
    47281 gcgagctaat aatgctttgt cggcatccaa ctccgtcggt aattcatagc ctgtttttcc
    47341 cagatattca gcaggaataa tgacaactgg catcgccata tcgatacagg tcaccgggac
    47401 atcgtcaaaa taatcaatct gattatccgt cgggaagact tttccggttt tggttccggc
    47461 ggcattcagg aaagtgagcg caaccggtgc ggcagtaccc ggtacgccgt caattctggc
    47521 gctgccctcg tactcgacaa caccatttgg cgtttgcaca tcagcttcgg tgaacgtacc
    47581 cgtattgacg ttgcggatac gtacgcgggt aactggcgaa gtcgctgcaa tcaaaccatt
    47641 ttcaatggcg aatgccccaa cgccagacag catattgccg cagttaggcg tggtatcgac
    47701 acgttgctca tggacgataa cttgagcaaa cagataatcg acatctgcac gcggatcgct
    47761 ggagcggcta ataatggcga ctttactggt cagcggatta ccgccgccaa taccgtcaat
    47821 ttccagatcg ttaccggaac ccataattgc catcaatatt ttatcgcgct gcgtttgatc
    47881 ttcgggtaga tgttccgcta acaggaacgc gcccctcgag gttccacctc gcatcatcac
    47941 gcagggtatt tttttcataa taatgccctc aatttaaaaa cataacttat tgcgatatgg
    48001 ttacattaag ggcaaagcat ctctaatgac tttagctcaa cgattaataa cattttttca
    48061 ttaatttaag ttggttaaat gcagttaaaa tttttaacgg tcagccaccc aaaatcgacg
    48121 cttccatttt ccggagtaat aatgaagcat gaattatcaa gtatgaaggc atttgtcata
    48181 ctggcagagt ccagttcatt taacaatgcc gctaaattac tcaatattac gcaacctgca
    48241 ttaacgcgaa gaataaaaaa gatggaggaa gatttacata tccaactatt tgagcgtaca
    48301 actcgcaagg ttacgttaac aaaagtagga aaaagattgc tcccggaggc gcgggaatta
    48361 ataaagaaat tcgatgaaac gctttttaat attcgtgata tgaatgctta tcatcgtggt
    48421 atggtgacat tagcgtgtat tccgaccgca gtattttatt ttttaccgct ggcaatcggt
    48481 aaatttaatg agctgtatcc caatattaaa gtgcggattc tggaacaagg cacgaataat
    48541 tgcatggaat cggtgctgtg taacgaatca gactttggca taaacatgaa caacgtcaca
    48601 aattcatcca tcgattttac cccactggtc aacgagccgt tcgtgctggc ctgtcggcgt
    48661 gaccatccat tggtcaaaaa gcagcttgta gaatggcagg agttagtggg ttacaaaatg
    48721 attggcgtac gctcctccag cggcaaccga ctgttgatag aacaacagct agccgacaag
    48781 ccctggaagc tggactggtt ttacgaagtg cgtcatcttt cgacgtcatt aggactggtg
    48841 gaggcaggac tggggatttc ggcgcttcct ggtctggcaa tgccccatgc gccctattct
    48901 tcgattattg gtatcccact ggtggaaccc gttatacgtc ggacattggg gattattcgc
    48961 cgcaaagatg ccgtgctttc tccggcagct gaacgctttt ttgccttgct gattaatttg
    49021 tggactgacg ataaagacaa tttgtggacc aatatcgtcg aacgccagag acatgcgtta
    49081 caggagatag gttaatacac cggcgcagga gagactgcgc cgggtaaatc agcggttagt
    49141 gtgcgttaac caccacccac attggtccct gcccgaccgc atagcggcct ttttcatgca
    49201 gtagcccctg ctcgccaaca atttcgtata ccgagatgtg gtgagatttt tgcccggcgg
    49261 caatcagata cttgccgctg tgatcaacat tgaagccgcg cggctgggtt tccgttggct
    49321 ggaagccttc tttactcaac acgctgccat cttccgaaac gctgaaaacg gtaatcaggc
    49381 tggcggtacg gtcgcaggcg tataaatgac gaccatccgg ggtgatatga atatcagccg
    49441 cccaacgggt gtcggagaag ttttccggca tcatatccag cgtctggaca cattcgatat
    49501 tgccgtgcgg atctttcagt tcccagacat ccactgagct gtttaactca ttgacgcaat
    49561 acgcatattg ttcgtttgga tggaatacca tatgacgcgg gccggcccct tcaacggtag
    49621 tcacttccgc agggtcctgt gccaccagat gaccatcatc gctgaccgta aacagacaaa
    49681 tgcgatcctg ctttaatgcc ggaacccaca gcgtacggtt gtccggtgag atattggcgg
    49741 aatggcaacc atccagcccc tcgaccacat cgacgacgcc cactggcagg ccatcttcca
    49801 gacgcgttac gctcacgtta cccgcattgt aagaaccgac aaagacaaac tgcccctggt
    49861 gatcggtgga aatatgcgtc gggctacccg gtagcgcaga ctctgcagca aaggtcagcg
    49921 cgccatcgtc cggggcgata cgatacgcca ggacgcgaaa ctcagggcgc acaccaacat
    49981 agagataacg tttgtccggg ctgaccacca tcggctgcac ctgccccggc acatcgacaa
    50041 cctgtgtcag cgtcagtgcg ccttcatgat tcaggttcca gacgtgaatt tgctggctct
    50101 cagggctggc gatataaact gtttgcttca tgaatgctcc tttgcattta gcaactgact
    50161 gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc agaattttgt
    50221 ccgatagtgg tatcggtgta ccattcgcgc agaagaaaat tcttaacctt aatctggaat
    50281 acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg accccgaaaa
    50341 agaccttgct tccatcatcg atagaagccc tggcccgcgc tcgcgaagca ggctatcaat
    50401 taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag gcgctggcgc
    50461 tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat gcaaaaacag
    50521 tgctggaagc ggacccaatg cccgttaata aagccctgca actcattgag atgctgaatg
    50581 aacaccacat tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg
    50641 ggcatgtcat tcgcacgtct aactgggcgc aaaccctgcc gccggaacag cgtccgactt
    50701 tcacacaagt cgcttctctg gctgaaacgg cgcaacaagt taacgccgta tggaagttcg
    50761 ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac
    50821 tgggacttga gtgtgaatgg tcctggcacg atcaggttga tattgcacgc ggcggcaaca
    50881 gcaaaggtaa acgtttggcg aaatgggttg aggcacaagg ttggtcgatg gaaaacgtcg
    50941 tggcattcgg cgataacttt aatgatatca gtatgctgga agccgctggt acaggcgtgg
    51001 cgatgggcaa cgccgatgat gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca
    51061 ccaccgacag cattgcccgg ttcatttata gccacctgat ttaatcaggc ggttatcgac
    51121 acacttttaa tttgcgcgta cagccacagg cctggtttga tcgccagttc atccctggcc
    51181 cacgggctga tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacctgg
    51241 ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc
    51301 tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct
    51361 tgcagcggtt cgtccagctt attgacccac aaatgctgat cgcccagcgc cagcgcggtc
    51421 atcgcgtaat gcggatgatg ctcaagcacc gtcactttca gaatgctact ttgttgctct
    51481 ttcggcagcc acggattcat cacgctactg ccccacactt cctccagcgc gccaaaggct
    51541 ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc
    51601 gaatggctga catacaacat cggaatgttg atttcccgtg tcagccgttg cagataaggc
    51661 aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat
    51721 tccggtgctg tcagcaaagc ccgaccaatc gccacgcgct gtttttcgcc tccggacagg
    51781 ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac
    51841 tgatcgacca tacttttcga catgccgtag cgcagattgc cacgcacttt gtaatgcggg
    51901 aacagccgtg catcctgaaa aacatagcca acgcgacgct tttctggcgt caggcagata
    51961 cctttttcgg catcatttag tacccgccca ttgaggacaa tccgcccttt ttgcgggcgc
    52021 gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata
    52081 gcagtgatgc cattggcggg cagcgtttca ttaatggtca ggcaatggtt gcccaacgtc
    52141 tgggaaaaat tcagttccag catgattagc gccccgcccg ttcacggctg attctggcca
    52201 gccattctga aatcaacagg gagatcatcg ccagtgcaat agaaataatg cacagtctcg
    52261 ccgcgccact ttccccgccg ggagtctgga tcagggtata catggcagaa ggaatggttc
    52321 gcgtttcacc aggaatgttc gacacaaagg tgatggttgc accaaactca ccgagagaac
    52381 gagcaaaagc cagtaccgta ccaacaataa tgcccggtaa ggtcagcggt aacgtgatag
    52441 taaagaaaac gcgccagcgc ccggcaccca gtgttcttgc ggcctgttcc agtttgacat
    52501 caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg
    52561 cagcgagaac cgcgccgcgc cagctaaacg cgaaagaaag accaaaccag tcatacagac
    52621 gttcaccgat aaatccgcgc cgtcccatcg aaactaataa taagtaaccg acgaccacgg
    52681 gcggtaacac cagcggtaga tgcagtacgc tatcgagcag agctttgccc ggaaacgtgc
    52741 aacgtaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg
    52801 aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt
    52861 ggttgtaaat ccgtaacgtt taaagatttc cgcagcctgc ggccccttca gataatcata
    52921 aaatgctatc actgtcgcat tattatgccc ttccacaaca gcaaccggat attccacttt
    52981 tttatgtgaa tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc
    53041 agaaccgtaa acaataccca gcggcgcttc attgcgttcg accaacgcca gcgcgccacg
    53101 aacatcttcc gccggagcca gtttcgggga gagcgtatcc catgcgccca gtttttgcag
    53161 tgcttctttt gcataaatgc cagcagggac atgttccgga tcgccaaccg cgaggcgacc
    53221 gccattcagc agcgaagtcc agttagtttt gctgtcgatg gtgaaatctt tctgctcgct
    53281 ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctggcgcg aagccgtatc
    53341 gatcgctttt ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc
    53401 cgcaggcgca cccgcttcaa tctgacgggc caaagtagat gacgaagcga aagaagaaac
    53461 cacatccacg cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag
    53521 tgatgcggcg gcgaacaccg tgattttccc ttcatctgcc agtgcattgc cagcaacagc
    53581 gaaagagagt gccgccccgg taaacaagtt caaccattta cgagccattg gtaacccctt
    53641 aatctaacgt tatgtaggcg tcaatataac ggcaagttcg gaattttccc agcagttatt
    53701 tctaaccttt gtgaggtaga tgagaaaaaa tatcggcagg aaaagcagga agttgagaaa
    53761 aagaaaatgc ccgactaagc gggcattcag ggaatcaatg attttgtccg ggctggtctt
    53821 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
    53881 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
    53941 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcac agaaaagcac
    54001 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc
    54061 caggatattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
    54121 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
    54181 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgaaatgaat
    54241 cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggcggcgcg
    54301 atgctgactc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
    54361 caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
    54421 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc
    54481 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga tggaaaaaca
    54541 cgcctgaaag tcgcaattac cgcacaaagc ggcgcgcgtc tggggctgga tgaaggcaaa
    54601 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcgatcgcg
    54661 caaaacgccg acaaccaatt accgggtatt attagtcata ttgagcgtgg cgcagagcag
    54721 tgcgaagtaa taatgtcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
    54781 gaagcgactt ctcttcagca aggacagaat gtcacggcct actttaatgc cgacagcgtg
    54841 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
    54901 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
    54961 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
    55021 ttgggcgttt gtcggttcga atggaagcgg gaaatcggcc ctggcccgcg cgctggcggg
    55081 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
    55141 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcag cggaataaca ccgatatgct
    55201 tagtcctggc gaagatgaca ccgggcgtac cacggctgag atcattcagg atgaagtaaa
    55261 ggatactccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
    55321 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
    55381 gtcggagcct gacttgttga tccttgatga accgttcgat ggcctggatg ttgcctcacg
    55441 tcagcagctg gctgagctac tcgcctcgtt acatcagtcc ggtattactc tggtactggt
    55501 gctcaatcgc ttcgatgaga taccagaatt tgtccagttt gctggcgtgc tggcggattg
    55561 tacgttagcg gaaactggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
    55621 ggcgcatagc gaacagcttg aaggtgtaca actgccggag ccggatgaac cttcagcacg
    55681 tcacgcctta cccgccaacg aaccgcgcat tgtgctgaac aatggcgtgg tttcttataa
    55741 tgatcgcccc attcttaata accttagctg gcaggtgaat ccaggtgaac actggcaaat
    55801 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
    55861 gcaaggttac agcaacgatt tgacgctttt cggacgacgt cgcggcagcg gcgaaaccat
    55921 ctgggatatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
    55981 cagtactact gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
    56041 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
    56101 taaacgcacg gctgacgctc cgttccatag tctttcctgg gggcaacaac gcctggcgct
    56161 gatcgtccgc gcgctggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
    56221 gcttgatccg ctcaatcgcc agcttatccg ccggtttgtt gatgtgctga ttagcgaagg
    56281 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
    56341 tcgtcttgag ttcgtgccag acggcggact ctatcgctat gtgctgacaa aaataaattg
    56401 agtcggtagt gctgaccgtg ccggaggcgg cctaagcgcc ctctccggcc aacggttcga
    56461 cgcatccagg catgaaaccg cgtctttttt ttcagataaa aagcgcaatc attcataaac
    56521 cctctgtttt ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat
    56581 tccactaatt tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata
    56641 ccataagcct aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg
    56701 gaagtcatac ctgtgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc
    56761 tctgtaacag taagcgcagc gtactgcccg ttatcgagcg tttaggcggc aaacatccaa
    56821 cgtttgttga aggcgatatt cgtaacgaag cgttgatgac cgagatcctg cacgatcacg
    56881 ctatcgacac cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac
    56941 cgctggaata ttacgacaac aatgtcaacg gtactctccg cctgattagc gccatgcgcg
    57001 ccgcgaacgt caaaaacttt atttttagct cctctgccac cgtttatggc gatcagccca
    57061 aaattccata cgttgaaagc ttcccgaccg gcacaccgca aagcccttac ggcaaaagca
    57121 aactgatggt tgaacagatc ctcaccgatc tgcaaaaagc ccagccggac tggagcattg
    57181 ccctgctgcg ctacttcaac ccggttggcg cacatccgtc gggcgatatg ggcgaagatc
    57241 cgcaaggcat tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg
    57301 actcgctggc gatttttggt aacgattatc cgaccgaaga cggtactggc gtacgcgatt
    57361 acatccacgt aatggatctg gcggacggtc acgtcgtggc gatggagaaa ctggcgaaca
    57421 agccaggcgt acacatctac aacctcggcg ctggcgtagg cagcagcgtg ctggacgtgg
    57481 ttaatgcctt cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg
    57541 agggcgacct tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc
    57601 gcgtgacgcg cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc
    57661 cacagggata tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac
    57721 atcgccgcta caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc
    57781 gcccctggca gggggcgcag gaaacgccag ccaaacaggt gttacctgcg catgatccag
    57841 attgcttcct ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg
    57901 ggacttacgt tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag
    57961 aaagcaaaga tccgctgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct
    58021 tttcaccgga tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg
    58081 tcaaaacctg gcaggagcaa accgcagaac tggggaaaac atacccatgg gtgcaggtct
    58141 ttgaaaacaa aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg
    58201 caaatagctt cctgcctaac gaagctgagc gtgaagaccg cctgcaaaaa gaatattttg
    58261 ccgaacagaa atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc
    58321 gtaccgttgt cgaaaccgaa cactggttag ccgtagtgcc ttactgggct gcctggccgt
    58381 tcgaaacgct actactgccc aaagcccacg ttttgcggat caccgatttg accgacggcc
    58441 agcgcagcga tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc
    58501 agtgctcctt cccctactct atgggctggc acggcgcacc gtttaatggc gaagagaatc
    58561 aacactggca gctgcacgcg cacttttatc cgcctctgtt gcgctccgcc accgtacgta
    58621 aatttatggt tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg
    58681 cagcagagcg tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa
    58741 tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc
    58801 acaccattca ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg
    58861 gtttcgttct gccctgcgcg attgattatc aaaccgtgat cagttgtgcg ccacgcgatg
    58921 accgtaaagt tcgcgtaatg gcagccgatt atgaaaatca gctcgacgag ttttcccttg
    58981 atgcgcccat tgtcgcacat gaaaactatc aatgggctaa ctacgttcgt ggcgtggtga
    59041 aacatctgca actgcgtaac aacagcttcg gcggcgtgga catggtgatt agcggcaacg
    59101 tgccgcaggg tgccgggtta agttcttccg cctcactgga agtcgcagtc ggaaccgtat
    59161 tgcagcagct ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggccaggaag
    59221 cagaaaacca gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg
    59281 gcaagaaaga tcatgccttg ctgatcgatt gccgttcact gggtaccaaa gcagtctcca
    59341 tgccgaaagg tgtggctgtc gtcattatca acagtaactt caaacgtacc ctggttggca
    59401 gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag
    59461 ccctgcgcga tgtcaccatt gaagagttca acgctgttgc gcatgaactg gacccaatcg
    59521 tggcgaaacg cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg
    59581 cgctggagca aggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta
    59641 tgcgcgatga tttcgaaatc accgttccgc aaattgacac tctggtagaa atcgtcaaag
    59701 ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg
    59761 tcgcgctgat cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg
    59821 aagcaaaaac aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac
    59881 agtgctgaac gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt
    59941 gcgtaacaac gcagggatgg tagtcacgct gatggactgg ggttcgactt tactttccgc
    60001 ccgtattccg ctttccgatg gcagcgtccg agaggcgctg cttggctgcg ccagcccgga
    60061 atgctatcag gatcaggccg catttctggg ggcctctatt ggtcgttatg ccaaccgcat
    60121 cgccaatagc cgttatacct ttgacggtga aaccgtgacg ctttcaccaa gtcagggcgt
    60181 taaccagctg cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca
    60241 gaatgatcgt caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg
    60301 taatctcggc gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta
    60361 tcgcgccaca gttgataaac cttgcccggt gaatatgact aatcacgtct atttcaatct
    60421 tgacggcgag cagtctgacg tgcgcaatca caagttgcag attctggcgg acgaatatct
    60481 gccggttgat gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacgtcttt
    60541 tgatttccgc agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa
    60601 agtgaaaggt tacgatcacg cgttcttgtt acaggccaaa ggcgatggca agaaagtggc
    60661 ggcgcatgtc tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctccggc
    60721 tctgcaattc tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta
    60781 cgccgactgg caagggctgg ctctggaaag cgaatttctg ccggacagcc cgaaccaccc
    60841 tgaatggccg caaccagact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga
    60901 atatcagttt attgctcagt aatgttaagc cctcgtcatg agggctttat cctcatattg
    60961 ttcaaatcac cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact
    61021 gcgacactat tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat
    61081 aatgagaatt attatcatta aaagatgatt tgaggagtaa gtatatggct gtaactaagc
    61141 tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt
    61201 acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga
    61261 aagaggaagg ttacagcttt gactttgctt acacctctgt gctgaaacgc gctattcata
    61321 ccctgtggaa tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga
    61381 aactgaacga acgtcactac ggtgcgttgc agggtctgaa caaagcggaa actgctgaaa
    61441 agtatggcga cgagcaggtg aaacagtggc gtcgtggttt tgcagtgact ccgccggaac
    61501 tgactaaaga tgatgagcgt tatccgggtc acgatccacg ttacgcgaag ctgagcgaga
    61561 aagaactgcc actgacggaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga
    61621 atgaaactat tctaccgcgt atgaagagcg gtgagcgcgt aatcatcgct gcacacggta
    61681 actcgttacg tgcgctggtg aaatatcttg ataacatgag cgaagaagag attcttgagc
    61741 tgaatatccc gaccggcgtg ccgctggttt atgagtttga cgagaacttc aaaccgctga
    61801 aacgctatta tctgggtaat gctgacgaga tcgcggcgaa agcagcagcc gttgcgaacc
    61861 agggtaaagc gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc
    61921 ggcttttttg tttgtcgttg ctaacaatgg agcacattgc ctgatgctac gctgcgcgtc
    61981 ttatcagggc tacggtgatt catcggatat gccactctga cggcgcatcc gacaattaaa
    62041 ccttacccgc gacgcgcttt tactgcattc gccagttgac gtaacagagc atcggtatct
    62101 tcccagccga tgcaggcatc agtgatgctc ttgccatagg ccagcggttc cccactctcg
    62161 aggctctgat tgccttccac cagatggctt tccaccatca cgccaataat ggccttttcg
    62221 ccaccggcaa tctgctggca aacgtcagca caaacatcca tctgcttttt gaattgtttt
    62281 gacgagttag catggctgaa atcgatcatc acctgtgctg gcaggcctgc tttgttcagc
    62341 ccttctttca cttcagcaac gtgcttcgcg ctgtagttag gctctttacc accgcgcaga
    62401 atgatatggc aatcgccgtt accgctggta ttcacaatcg ccgaatgccc ccatttcgtt
    62461 acggacagga agcagtgcgg cgtaccggcg gcattaatgg catcgatagc cactttaatc
    62521 gtaccgtcgg tgccattttt gaagcctacc gggcaagaaa gccctgatgc cagttcgcgg
    62581 tgcacctgcg attccgtagt acgtgcgcca attgcgcccc agctcatcag gtcagcgaga
    62641 tattgtgggg tgatcatatc caggaattca cccgccgctg gcagaccgct gtcgttaata
    62701 tcgagcagca atttgcgggc aatacgcaga ccgtcgttga tctggaagct gttatccata
    62761 tgcggatcgt taatcagccc tttccagccc accgtagtac gcggcttttc aaaatagacg
    62821 cgcatcacga tttccagctc atcttgcagc tcttcacgca gcgtcagcaa gcgagcggca
    62881 tactctttag ccgcgacagg atcatgaatt gagcacggac caatcaccac caacaggcga
    62941 tcatcattac ctttcaggat tttatggatc gcttttcggg catgggcgac cgtattcgcg
    63001 gcattttcag tagcggggaa tttttccagc aatgcgacag gaggaagtaa ctctttgatt
    63061 tctttgatgc gtaaatcgtc gttctgataa ttcatgtctg ttccagtgtt gccatactta
    63121 tcttagtgaa tgcaatactt ccaatctata tcttccgtca gaatgtgtaa acggggtttt
    63181 acactatgaa cggattaatc ctggaattag caagaaaaac gccagattgt cgcgaaaaac
    63241 gagatctctc ctacaatttc taactgtaac tcctttgttt gttaattatt tcaagattct
    63301 ctgctgggta tctttgcttg gctgaaatct taaaccattg ccttatattc acctgcaaat
    63361 gcactgttgg aagaggttat ccgacataac gaccataaca ggagcatcct atgaaaatga
    63421 caaaactggc cacactattt ttgactgcca ctctaagcct tgccagcggt gccgcactgg
    63481 ctgccgatag cggagcgcaa agcaataacg gccaggcaaa cgccgcagct gatgcgggcc
    63541 aggtagcccc tgatgcccgt gaaaatgtcg cgccaaataa cgtcgacaat aacggggtaa
    63601 ataccggttc tggcggcaca atgctgcatc cggatggttc ttcaatgaac aatgacggca
    63661 tgaccaaaga tgaagagcac aagaacacca tgtgcaaaga tggtcgctgt ccggatatca
    63721 ataaaaaggt acaaactggt gatggcatca acaatgatgt cgataccaaa accgacggta
    63781 ccacgcagta accctgcgaa atagccaata atgggagagc ggagtctctc ccttttaatt
    63841 tttcccttct aaaagcagta tgatgtcata aagttaagtg agaataatta agggatgact
    63901 cgtcatggcg cactcacact cacacacttc ctcacacctg ccagaagata ataatgctcg
    63961 tcgcttgttg tatgctttcg gcgtgactgc cgggtttatg ctcgtagaag tcattggtgg
    64021 ttttctttcc ggttctctgg cattgctggc cgatgcgggt catatgttga ccgatactgc
    64081 cgccctgctt ttcgccctac tcgcagtgca attttcccgt cgtcctccca ccattcgcca
    64141 cactttcggt tggctaagat tgaccacgct cgcggcgttt gtgaatgcca tcgccctggt
    64201 ggtgattact attttgattg tctgggaggc gatagaacgc ttccgcactc cgcgtccggt
    64261 cgagggcggc atgatgatgg caattgccgt ggctgggctg ctggcaaata tactttcttt
    64321 ctggttactt catcacggca gcgaagagaa aaacctcaac gtgcgagcag cggcactgca
    64381 tgtgctcggg gacctgctgg gttcggttgg ggctattatt gccgcgttga ttattatctg
    64441 gaccggctgg acacctgctg accccattct ctcaatactg gtgtcgcttc tggttctgcg
    64501 tagcgcgtgg cgactgttga aagatagcgt gaatgaatta cttgaaggtg caccggtatc
    64561 gctggatatc gccgaactga agcgtcgtat gtgccgggaa atctcggaag tccgcaatgt
    64621 gcaccatgta catgtatgga tggtaggcga gaagccggtg atgacgctgc atgtgcaggt
    64681 gatcccgcca cacgatcacg atgccttgtt agatcagatc caacactacc tgatggatca
    64741 ctatcagatt gagcacgcca ctattcagat ggaatatcaa ccttgtcatg ggccagactg
    64801 ccacctgaat gaaggggtgt ctggccattc acatcatcac cattaatggg acagcgcgcg
    64861 tgaacctctt tcgcgtgcgc tgttgatcca catccggctg ccgttaagcg caataaaggt
    64921 caggatgatg tactccagag acatggcgta aacgccctga agtgcaaaaa taacgacgct
    64981 aatcacgtta ataatcaccc acaacagcca gttttccaca tacttacgcg tcatcagaat
    65041 cattgccacg atggataaca ccatcatgca tgaatcccag aacgggaaag cgtccggctg
    65101 tagttcaggc atcgcaacct gtaatcctaa tgcttgcatg atcatgactg ccacgcgggt
    65161 caaaaatgca aacaccggat tgataaatac cgtcatcaga ccaatcgaaa caacgcaaac
    65221 cgccaaccag ctgagcgcct tcggcaatgg caaccagcga attttcaact ccgcctcgtt
    65281 ctgactggtt tgtcgcgacc acgcatacca accgtaaata ttcgcggcaa agaaaaacac
    65341 ctgtaatagc aggctggcat acagctgaat ctgaaagaaa ataatgccaa acaaggtgac
    65401 gttgatcagg ccaaagaagt agttgctgat cttctccata ctggcaaggc caatacacag
    65461 caacccagcg atcgtgccta ccgcttcgat ccatgagaga tcataaccgc ctgctcctat
    65521 tggtatatgt accaggatat tctgcacact aaaaaaatcc atctttcccc caaagcgtaa
    65581 cgttacgcct tattattcgt tatccacgta gtgtagccgc aaaatccagc atacgattga
    65641 gcggcaccag cgccctctct cgcagccttt catcaacatg aacctcgtga ttgcttcctt
    65701 cctgttctaa tgcctctgcg atggcctgaa ggccattcat ggccatccac ggacaatgcg
    65761 cgcagctgcg gcaggttgca ccctcacctg cggttggtgc ttccagtaac tctttatctg
    65821 gcaccgcctg ctgcattttg tagaaaatac cccgatcggt tgccacaata agcctctgat
    65881 gtggcaatgt tttcgcagca gcgatcagtt gactggtgga accgaccgca tccgccatat
    65941 cgacaatagc ttgtggtgat tctggatgca ccagtatggc agcatccggg tattcttctt
    66001 gcaagcgggt taacgcctga gtcttaaatt catcatgcac aatgcaggca ccctgccagc
    66061 ataaaatgtc tgcgcccgtc tgtttttgca cgtaacgccc cagatgtttg tcgggagccc
    66121 agatgatttt ttcacccaaa ctatccagat gatcaataag ttcgacggca atgcttgaag
    66181 ttaccaccca atctgcccgc gcttttaccg cagcagaagt gttggcgtag acaacgacag
    66241 tatgatcggg atgggcatcg caaaatgcgt taaattcttc aacagggcag ccgagatcca
    66301 gtgaacattc agcctgaagt gtcggcatca gaattgtttt ttccggactg agaattttgg
    66361 cggtttctcc cataaatctc accccagcga ctaacaacgt agaagcggga tgctttgcac
    66421 cgaagcgcgc catttccaga gaatcagaaa tacagccacc ggtttcttct gccagttgtt
    66481 gaatttcggg atcggtatag taatgggcaa ccatcaccgc attacgttct tttagcagac
    66541 gttttatctt ctcgcggtaa tacgcttttt catcaatgct taacggcgtc ggcttcgggg
    66601 ggaaaggata aatcgccgtg tctggatcaa acatcacgct catcttacca tctcgtttta
    66661 caggcttaac gttaaaaccg acatgagcgt aatattcgct gttatcggtg aaatttgttt
    66721 tatatgctaa acaagatagt cgaatggtgg ggaaaagtca ctcgaattac gtatgagatg
    66781 acgaatgcca cactttgcgt cttattcctc cgacgtagag ttgtttacgc agcaagcgcg
    66841 gagtatgtca tcagggttaa gaattgaaat tgagagatgc ctgaatagca aaaaagcgcc
    66901 tttagggcgc ttttttacat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    66961 aaaagtcaac tgctctacca actgagctaa cgaccccttg cgggattatg ctgctttaat
    67021 cattcaggat ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
    67081 gctctaccaa ctgagctaac gacccatacg ggtgttacct gaaagatttc tactcggaac
    67141 caatctttaa aattggtggg tcgtgcagga tgactcgctt cgctcgccct tcgggccgtt
    67201 gctggcgcaa cgttatcctt cacgcttaac atctgagttt gatgttaaaa ttggtgggtc
    67261 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    67321 acgacccgag tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    67381 tgctctccca gctgagctaa tcacccgata ctgcgctgga tactacaaac tacttccacc
    67441 gaacccaccg taattagtgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
    67501 agtcaactgc tctaccaact gagctaacga cccggtggtg ggtgatgacg ggatcgaacc
    67561 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactacg
    67621 ctggatacta caactaacta ggttagtggt gggtcgtgca ggattcgaac ctgcgaccaa
    67681 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccactttttc gttgctttcg
    67741 gtttgtttga tatcccgtgg caacggcggc atatattact gatttcagac ttgagcgcaa
    67801 caaaaatttc gatgtagatc actcaactgc ttatgattcg cacgacacga ccagaaataa
    67861 tgcgacttct ggtcgtgtgt tatgcattac atcgcgttaa gacgtttttg tgcctgttta
    67921 gcgccatcag taccagggta tttactgata acctgctggt atacggcttt cgcttttgcg
    67981 gtatcacctt tgtcctgcat gatgacgccg actttaaaca tcgcatctgc agcctttggt
    68041 gacttcggat agtttttcac taccgaagca aaataatacg ccgcatcatc ttttttaccc
    68101 ttgttgtagt ttaactgacc cagccaataa ttggcgtttg gcaggtaagt tgaatcaggg
    68161 taatttttga tgaaattctg aaatgccacc attgcgtcat cctggcggga tttatcctgc
    68221 accagcgcaa tagctgcatt gtaatccgtg tttgcatcac cgcttttcac cggcgcgcca
    68281 gcattcgcag taccagcatc agctgtcggc gtcgttgatg ccgccgcacc gctttgatcg
    68341 ccgctggttg attgcgccgc tgcaccaccg ctgctgaggc tatcgatctg caacaggatc
    68401 tgcttttgcc gctccacgac ctgattcagt tgatactgat tttcctgaat ctgaccacgc
    68461 agggaatcaa tatcggattg attatcagag agttgttgct ggagttgggt taaaagctgg
    68521 ctgtgagcat ttgaaatacg ctcaagttga gtgacgcggt cttcgaccga gcctgagccg
    68581 acactactga ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc aaccagtaac
    68641 gacagactca atagttgatg tctgaagtta ctgctcatgc aattctctta gtaaaccagt
    68701 accgcacgac ggtttttgga gtatgccgct tcgtcatgac ccagtactgc aggtttttct
    68761 ttaccgtaag aaacgatgga gatctggtct gcagaaacgc ctttaccctg caggtacatc
    68821 ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt attccggagt accacgttcg
    68881 tccgcgtgac cttctacggt gactttgtaa gacgggttgc tacgcaggaa gtttgcgtgt
    68941 gcatccagca tttgagcgaa gtcagaacgg atatcgtact tgtccagatc gaagtaaacg
    69001 atgttgttct gctgcagctg ttgcatttgc agacgagcct gctcttcgga agacatgttg
    69061 ccattgccgc cgttcgcatc cataccagtg ccggcaccca gcatgccttc gctgccgtca
    69121 ttgctggcgt tcttgttgga agaacatgcc gcaattgcca taacaggcag agcaatcatc
    69181 agccctttca gcactttgtt cagttgcatt tcaatgattc ctttactatt caattaatta
    69241 ttatcacaga tacggcgacc aggcagggaa tttgacctgt ccatcagttg ccggaagacg
    69301 cgctttgaaa cgcccatctg tagaaaccaa attcagcacg gatcccatcc cctgagaaga
    69361 gctgtagatt accatagtgc cgttaggtgc cagacttggc gtttcatcca ggaacgtgga
    69421 cgacagaact tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct gctgcccacc
    69481 attggagctg accattacca taaatttacc gtcgctgctg acatccgcat cctggttctg
    69541 cgaaccttcc caggtaatac gttgtggcgc accgccgttg atattcactt tataaacctg
    69601 tggacgaccg gcctggtcag aagtaaatgc caggttctgg ctgtccggga accaggtcgg
    69661 ttcggtattg ttactgcgac catccgtcac ctggcggatc tgaccagaag ccaaatccat
    69721 tacgtacagg ttcagactac cggttttcga caaggcgaat gccagtttgc tgccgtctgg
    69781 cgagaatgca ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta cagcgccatt
    69841 tgccagcgtt tggatgacca gcgcggaacg accgctttcg aaggtcacat aagccagttt
    69901 agaaccgtct ggtgaccacg ccggagacat cagcggctgc ggtgaacggt gaacgacaaa
    69961 ctggttgtaa ccgtcatagt cagatacgcg cagttcatac gggaactgac cgccgttggt
    70021 ctgaacaacg taggcaatac gggtacggaa cgcaccttta atgccggtca gtttttcaaa
    70081 cacttcatca ctggcggtat gaccagcata acgcagccac tgcttgttca ctttgtacga
    70141 gttctgagca agtacagtac ccggtgcgcc gccagtgtca acaagttgat aagcaacatt
    70201 gtaagaacca tccggattcg gagtgacctg accgacaact acagcgtcaa tacccagcgc
    70261 ggaccatgca gctggttgta cttcctgcgc actacccggc tgctgtggca gacgagcacg
    70321 atctaacgga ttaaatttac cgctgttacg caagtcagca gcaacgatgc cgccaatatc
    70381 ttcaggtgcc gcaccaggac ccgcccactg gaaaggaaca acaccaatag gacgaccgga
    70441 atctacaccg ctgtcgatca caatgcggac ttcagcatgc agaactgatg cccacagtat
    70501 gagaaaacca aatgctactc gtaatgcctg cttcatcata tctcccttat ctggaccgat
    70561 ggcccacgat aatttagcag aatgttaaca aactaaaata cacaaaacta ccagaacccc
    70621 gtggcaaccg gtgcctgatg ttgaccgtcg gaacagtcaa catcgcgatt acggtttgaa
    70681 gtccaatggc gcgtttttga acacttcata tactgcctgg cttggtggtt tcgggatctt
    70741 cgcaagttta gctgctgcca acgcagcctg acaaagtgcg ggatcgccac cttcaggttt
    70801 gatatccagt aacataccat cgggtgccag ttttatgcgc agcgtacagg ttttgcctgc
    70861 ataggacgat gcgtcataga acttactttc gatagcagat ttaatctgcc cggcatagtt
    70921 attgatatcg gcccctgatg cgccattgtt tttagtatta ccactcccgg ctggcgaagc
    70981 attgttccct ttcgcccctc cccccgtttt cggtgcattc ttaccagagc ttagctcacc
    71041 gaaaatatca tctgcctctg cggcagcttt tgctgcagcg gctttttcag ctgctgcttt
    71101 tgcagctgcc gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc
    71161 tgccttttca gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc
    71221 cgctttctta tcagctgcag ccttttcagc agccgctttc ttctcagctg cagccttttc
    71281 agcagccgct ttcttctcag cttctgcttt ggctttttca gcagcttcag ttgccgcttt
    71341 ctttcttgct tcagctgcag ctgcttctgc cgcttccgct ttcttcttca gtgccgccgc
    71401 ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt
    71461 tttcttcgcg tctgcaaccg ctttcttcgc agcttcttcc gcagctttag catccgcttc
    71521 ggccttcgct ttagcatctg ccgccgcttt cgctgccgct tcttccgctt gcttctgctt
    71581 taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgttcctgag cagctaaccg
    71641 ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc
    71701 agcagcctgc tgttctttca tcttgcgctg ctcatcagaa cgcttcgcgc ttgattcctg
    71761 gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat
    71821 ggatgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagat
    71881 cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt
    71941 gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc
    72001 gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg
    72061 atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat
    72121 cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt
    72181 ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt
    72241 actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct
    72301 gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg
    72361 ccataaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg ttgatttcgg
    72421 acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct
    72481 cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt
    72541 aattcagttc cagtttgttt acgcgctggt tgagacggtt ataagccatt accgccggaa
    72601 ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca
    72661 tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga
    72721 ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt
    72781 gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt
    72841 ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc
    72901 cgctgtaaaa gatttgttcc gaaccagtca gattatcccg tttcccctga ctctcttgat
    72961 agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcacgcg
    73021 ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaggatgcg attgaaaaac
    73081 caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga
    73141 tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt
    73201 cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc
    73261 ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt
    73321 ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg
    73381 taccacaaag gcaacgcgtt cagccatcag cgcctgttga ctgaagtgat gatgacgcag
    73441 catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc
    73501 ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt
    73561 acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat
    73621 tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc tggagcggcc
    73681 cataaatttt taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga
    73741 acaatatcgg caagcaaagg gcaaaaaatc ccttgccaga gaaccttctg cggacgaaag
    73801 cccacgccgt gaataacacc agcacagacg gcccacatca gcaataaacc atgccagatt
    73861 tccagttcac tggtcttcgc ggcgaaacgt gatgggtccc aaaacataca tcctgctaac
    73921 agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag
    73981 gttgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgct
    74041 caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg
    74101 cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc
    74161 ttctttggtg atacggccga acattttcca gtaacaccag gcggtgtaga gcagaatgat
    74221 cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc
    74281 atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat
    74341 tgcgataccg gcagtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca
    74401 cgcagcttta tccatacgag cagtcaggat ggtcagcagc ggcagaacta cacccagtgc
    74461 cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg
    74521 aaccacttct ttattcaacg ggttagaggc tgcgtaatgg tccattgttg atttcacaac
    74581 ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaagcaga ccagtgtcac
    74641 cagtgccgcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat
    74701 ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc cagccagcag
    74761 gccgaacggg ttaagcagct ggaagaagtt accggtgtag tacagacgca gatattcatc
    74821 aacgttgaac ggtacgccct gcaacaggtt accgaatgct acaccaatca ccagcggcgg
    74881 aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat
    74941 cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat
    75001 cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc
    75061 gccgcctgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcga tggagttaat
    75121 cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat
    75181 gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat
    75241 aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt
    75301 agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg
    75361 tgcaaacttg aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat
    75421 cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gagttcgcca cagctgtcgg
    75481 cagcacttca ccgatagccc acggttggcg gccatattca gccacaaacc agcccgcttc
    75541 tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagcagcc attttttctc
    75601 gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca gcagcaggaa
    75661 gccacacgcc accatgatac ggaacgcgaa gtacagcggc gctacacgcg gaatggagtc
    75721 tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt
    75781 cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgcctg
    75841 gtcggtagaa ccagaacgca gttgctcgag cagagagtac gccttcatcc cgttacgaat
    75901 gcgttcttca tgctgcacca tcagttcttt caggccgata actggagtat ccacggaacg
    75961 cgttgcaatg atacccagcg cgtaagggat ctggatcgca aatttgttcg tctcctcttc
    76021 ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt cccactcggc
    76081 ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtaaccag attcatcacc
    76141 cagaacaata acagacagaa cggcagccat accgaagctg gcagcgatag caaaggagcg
    76201 tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa
    76261 catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg
    76321 gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc
    76381 ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt
    76441 tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca
    76501 accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat
    76561 tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc
    76621 gaactggaac tccatagtca gaccggtagc cacacccaga gcgaagttga taccaaacaa
    76681 cttgccccag aacttggtca tatctttata aatctgtttg ccggaaagga cgtagaccgt
    76741 ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta
    76801 catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc
    76861 ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc
    76921 caa
//
LOCUS       DARNVM010000004        65017 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.004, whole genome shotgun sequence.
ACCESSION   DARNVM010000004 DARNVM010000000
VERSION     DARNVM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.004"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..278)
                     /gene="folK"
                     /locus_tag="SI621_000234"
     CDS             complement(<1..278)
                     /gene="folK"
                     /locus_tag="SI621_000234"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEI4062996.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPR"
     gene            complement(275..1693)
                     /gene="pcnB"
                     /locus_tag="SI621_000235"
     CDS             complement(275..1693)
                     /gene="pcnB"
                     /locus_tag="SI621_000235"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEI4062997.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRSRRPRKRAPRREGTA"
     gene            complement(1732..2658)
                     /gene="gluQRS"
                     /locus_tag="SI621_000236"
     CDS             complement(1732..2658)
                     /gene="gluQRS"
                     /locus_tag="SI621_000236"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEI4062998.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAADTILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2695..3150)
                     /gene="dksA"
                     /locus_tag="SI621_000237"
     CDS             complement(2695..3150)
                     /gene="dksA"
                     /locus_tag="SI621_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEI4062999.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3328..4032)
                     /gene="sfsA"
                     /locus_tag="SI621_000238"
     CDS             complement(3328..4032)
                     /gene="sfsA"
                     /locus_tag="SI621_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEI4063000.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4047..4577)
                     /gene="thpR"
                     /locus_tag="SI621_000239"
     CDS             complement(4047..4577)
                     /gene="thpR"
                     /locus_tag="SI621_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEI4063001.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4651..7080
                     /gene="hrpB"
                     /locus_tag="SI621_000240"
     CDS             4651..7080
                     /gene="hrpB"
                     /locus_tag="SI621_000240"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEI4063002.1"
                     /translation="MSSLPVAAVLPELLTALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMVVRAKSDDEAATAAKIAAILEEPPRVG
                     NCDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQIMRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7174..9708
                     /gene="mrcB"
                     /locus_tag="SI621_000241"
     CDS             7174..9708
                     /gene="mrcB"
                     /locus_tag="SI621_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HEI4063003.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9928..12186
                     /gene="fhuA"
                     /locus_tag="SI621_000242"
     CDS             9928..12186
                     /gene="fhuA"
                     /locus_tag="SI621_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HEI4063004.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEIQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGIARSANAQQKGAEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDDDALKASPCLSIPQSEWNHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVEHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPDPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWAEQSSFNRDYGNKSD
                     RDDKQFTWRGGVNYLFDNGVTPYFSYSESFEPASLTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVLTGALYQLTKTNNLMADPNNPNFSIEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12237..13034
                     /gene="fhuC"
                     /locus_tag="SI621_000243"
     CDS             12237..13034
                     /gene="fhuC"
                     /locus_tag="SI621_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HEI4063005.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13034..13924
                     /gene="fhuD"
                     /locus_tag="SI621_000244"
     CDS             13034..13924
                     /gene="fhuD"
                     /locus_tag="SI621_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HEI4063006.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            13921..15903
                     /gene="fhuB"
                     /locus_tag="SI621_000245"
     CDS             13921..15903
                     /gene="fhuB"
                     /locus_tag="SI621_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HEI4063007.1"
                     /translation="MSKRIALFPALLLALLVIVAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15938..17218)
                     /gene="hemL"
                     /locus_tag="SI621_000246"
     CDS             complement(15938..17218)
                     /gene="hemL"
                     /locus_tag="SI621_000246"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEI4063008.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17443..18864
                     /gene="clcA"
                     /locus_tag="SI621_000247"
     CDS             17443..18864
                     /gene="clcA"
                     /locus_tag="SI621_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HEI4063009.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18888..18953
                     /locus_tag="SI621_000248"
     CDS             18888..18953
                     /locus_tag="SI621_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063010.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            18946..19290
                     /gene="erpA"
                     /locus_tag="SI621_000249"
     CDS             18946..19290
                     /gene="erpA"
                     /locus_tag="SI621_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEI4063011.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19337..19960)
                     /locus_tag="SI621_000250"
     CDS             complement(19337..19960)
                     /locus_tag="SI621_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEI4063012.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(19998..20798)
                     /gene="btuF"
                     /locus_tag="SI621_000251"
     CDS             complement(19998..20798)
                     /gene="btuF"
                     /locus_tag="SI621_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HEI4063013.1"
                     /translation="MAKSLFRALVALSFLVPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20791..21489)
                     /gene="mtnN"
                     /locus_tag="SI621_000252"
     CDS             complement(20791..21489)
                     /gene="mtnN"
                     /locus_tag="SI621_000252"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEI4063014.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21573..23090
                     /gene="dgt"
                     /locus_tag="SI621_000253"
     CDS             21573..23090
                     /gene="dgt"
                     /locus_tag="SI621_000253"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HEI4063015.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLEEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23220..24644
                     /gene="degP"
                     /locus_tag="SI621_000254"
     CDS             23220..24644
                     /gene="degP"
                     /locus_tag="SI621_000254"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HEI4063016.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24799..25956
                     /gene="cdaR"
                     /locus_tag="SI621_000255"
     CDS             24799..25956
                     /gene="cdaR"
                     /locus_tag="SI621_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HEI4063017.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26010..26396)
                     /locus_tag="SI621_000256"
     CDS             complement(26010..26396)
                     /locus_tag="SI621_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HEI4063018.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26709..27533)
                     /gene="dapD"
                     /locus_tag="SI621_000257"
     CDS             complement(26709..27533)
                     /gene="dapD"
                     /locus_tag="SI621_000257"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEI4063019.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27564..30236)
                     /gene="glnD"
                     /locus_tag="SI621_000258"
     CDS             complement(27564..30236)
                     /gene="glnD"
                     /locus_tag="SI621_000258"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HEI4063020.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRHLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30298..31092)
                     /gene="map"
                     /locus_tag="SI621_000259"
     CDS             complement(30298..31092)
                     /gene="map"
                     /locus_tag="SI621_000259"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEI4063021.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31460..32185
                     /gene="rpsB"
                     /locus_tag="SI621_000260"
     CDS             31460..32185
                     /gene="rpsB"
                     /locus_tag="SI621_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEI4063022.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32443..33294
                     /gene="tsf"
                     /locus_tag="SI621_000261"
     CDS             32443..33294
                     /gene="tsf"
                     /locus_tag="SI621_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEI4063023.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33441..34166
                     /gene="pyrH"
                     /locus_tag="SI621_000262"
     CDS             33441..34166
                     /gene="pyrH"
                     /locus_tag="SI621_000262"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEI4063024.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34458..35015
                     /gene="frr"
                     /locus_tag="SI621_000263"
     CDS             34458..35015
                     /gene="frr"
                     /locus_tag="SI621_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEI4063025.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35107..36303
                     /gene="ispC"
                     /locus_tag="SI621_000264"
     CDS             35107..36303
                     /gene="ispC"
                     /locus_tag="SI621_000264"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEI4063026.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36492..37250
                     /gene="ispU"
                     /locus_tag="SI621_000265"
                     /gene_synonym="uppS"
     CDS             36492..37250
                     /gene="ispU"
                     /locus_tag="SI621_000265"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HEI4063027.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37263..38120
                     /gene="cdsA"
                     /locus_tag="SI621_000266"
     CDS             37263..38120
                     /gene="cdsA"
                     /locus_tag="SI621_000266"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEI4063028.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWSVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38132..39484
                     /gene="rseP"
                     /locus_tag="SI621_000267"
     CDS             38132..39484
                     /gene="rseP"
                     /locus_tag="SI621_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HEI4063029.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWTFEPDKEDPITSLGIRPR
                     GPQIESVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39514..41946
                     /gene="bamA"
                     /locus_tag="SI621_000268"
     CDS             39514..41946
                     /gene="bamA"
                     /locus_tag="SI621_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEI4063030.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42068..42553
                     /gene="skp"
                     /locus_tag="SI621_000269"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42068..42553
                     /gene="skp"
                     /locus_tag="SI621_000269"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HEI4063031.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42557..43582
                     /gene="lpxD"
                     /locus_tag="SI621_000270"
     CDS             42557..43582
                     /gene="lpxD"
                     /locus_tag="SI621_000270"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEI4063032.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSRIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43687..44142
                     /gene="fabZ"
                     /locus_tag="SI621_000271"
     CDS             43687..44142
                     /gene="fabZ"
                     /locus_tag="SI621_000271"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEI4063033.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44146..44934
                     /gene="lpxA"
                     /locus_tag="SI621_000272"
     CDS             44146..44934
                     /gene="lpxA"
                     /locus_tag="SI621_000272"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEI4063034.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44934..46082
                     /gene="lpxB"
                     /locus_tag="SI621_000273"
     CDS             44934..46082
                     /gene="lpxB"
                     /locus_tag="SI621_000273"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEI4063035.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46079..46675
                     /gene="rnhB"
                     /locus_tag="SI621_000274"
     CDS             46079..46675
                     /gene="rnhB"
                     /locus_tag="SI621_000274"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEI4063036.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPSRPIAGLN
                     DSKKLSEKRRLALCEEIKDKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46712..50194
                     /gene="dnaE"
                     /locus_tag="SI621_000275"
     CDS             46712..50194
                     /gene="dnaE"
                     /locus_tag="SI621_000275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEI4063037.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVMRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50207..51166
                     /gene="accA"
                     /locus_tag="SI621_000276"
     CDS             50207..51166
                     /gene="accA"
                     /locus_tag="SI621_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEI4063038.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51264..53405
                     /gene="ldcC"
                     /locus_tag="SI621_000277"
     CDS             51264..53405
                     /gene="ldcC"
                     /locus_tag="SI621_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HEI4063039.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53462..53851
                     /locus_tag="SI621_000278"
     CDS             53462..53851
                     /locus_tag="SI621_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEI4063040.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53916..55214
                     /gene="tilS"
                     /locus_tag="SI621_000279"
     CDS             53916..55214
                     /gene="tilS"
                     /locus_tag="SI621_000279"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEI4063041.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRAENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55263..55523)
                     /gene="rof"
                     /locus_tag="SI621_000280"
     CDS             complement(55263..55523)
                     /gene="rof"
                     /locus_tag="SI621_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HEI4063042.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55510..55710)
                     /locus_tag="SI621_000281"
     CDS             complement(55510..55710)
                     /locus_tag="SI621_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HEI4063043.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55876..56421
                     /locus_tag="SI621_000282"
     CDS             55876..56421
                     /locus_tag="SI621_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEI4063044.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56418..56840
                     /gene="arfB"
                     /locus_tag="SI621_000283"
     CDS             56418..56840
                     /gene="arfB"
                     /locus_tag="SI621_000283"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEI4063045.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56854..57564
                     /gene="nlpE"
                     /locus_tag="SI621_000284"
                     /gene_synonym="cutF"
     CDS             56854..57564
                     /gene="nlpE"
                     /locus_tag="SI621_000284"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HEI4063046.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLTARGHSEKPMLLSLEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57595..58419)
                     /locus_tag="SI621_000285"
     CDS             complement(57595..58419)
                     /locus_tag="SI621_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HEI4063047.1"
                     /translation="MDKPKVHCRLLLPCFLFLSACTVDISQPDSSATAVDAGAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSHGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58472..60190)
                     /gene="proS"
                     /locus_tag="SI621_000286"
     CDS             complement(58472..60190)
                     /gene="proS"
                     /locus_tag="SI621_000286"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEI4063048.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60301..61008)
                     /gene="tsaA"
                     /locus_tag="SI621_000287"
     CDS             complement(60301..61008)
                     /gene="tsaA"
                     /locus_tag="SI621_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEI4063049.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61005..61409)
                     /gene="rcsF"
                     /locus_tag="SI621_000288"
     CDS             complement(61005..61409)
                     /gene="rcsF"
                     /locus_tag="SI621_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HEI4063050.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61527..62342)
                     /gene="metQ"
                     /locus_tag="SI621_000289"
     CDS             complement(61527..62342)
                     /gene="metQ"
                     /locus_tag="SI621_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HEI4063051.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62382..63035)
                     /gene="metI"
                     /locus_tag="SI621_000290"
     CDS             complement(62382..63035)
                     /gene="metI"
                     /locus_tag="SI621_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HEI4063052.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63028..64059)
                     /gene="metN"
                     /locus_tag="SI621_000291"
     CDS             complement(63028..64059)
                     /gene="metN"
                     /locus_tag="SI621_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HEI4063053.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64246..64818
                     /gene="gmhB"
                     /locus_tag="SI621_000292"
     CDS             64246..64818
                     /gene="gmhB"
                     /locus_tag="SI621_000292"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEI4063054.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15305 a        16196 c        17971 g        15545 t
ORIGIN      
        1 cgcggccccc agcgttccgc tttacggacg cgaccttgtt gtaactcaat acgttgtgtg
       61 tggttgagta actcttcagg tgcaagagag gtttccagcg ccacggctgc gtttaagtaa
      121 tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa acgaagaaac ggcaagaatg
      181 tggctttcag ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag
      241 gccagattgc tgcctatggc aatatacgcc actgtcatgc ggtaccctca cgacgcggtg
      301 cgcgtttgcg tggacgacga gaacggcgac gcggtgacgg ctcttcatcc agctcgttga
      361 gcatcccttt ttggtctggt ggcgcggaaa cctggaactc accccaccat ttcacaagac
      421 gctgcagttc agcgttacgt tcaacttcag ctcgcaaggc caacaggtca taagccgcac
      481 ggaacttagg gtgctccagc agtttccagg cgcgtttacc ctgacgtcgg gacatacgca
      541 actgcaactg ccagatatcg cgggttaacg tcgtcagacg tttcgggatc gccagtgaac
      601 ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc
      661 cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa
      721 acaggaacgc cgggttcacg cgcatatcgt tatggatacg cgtatcggta ttcttcagca
      781 cctgttcaat gatccgctcc atcgggctgt cgccattttc cgtgaagtag cgggtaatgg
      841 tcgggaacag cggctggaac aggtgatatt cacacagcag cttataggtt tcgtaaccgt
      901 agcccgcttg cagcagtttc aaagattctt caaacagacg tgccggtgga atatcgttca
      961 gcagggtagc caggcgaggg atcggttctg cggtttcagg actgatacgc atacccaatt
     1021 tggcggcaaa acgtaccgcg cgcagcatcc gtaccggatc ttcacggtag cgcgtttccg
     1081 ggttaccaat cagacggata acgccgtcct tcagatcctt catgccgcca acgtaatcac
     1141 ggacggtaaa atccgctacg ctgtaataca ggctgttgat agtgaaatcg cggcgctggg
     1201 cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa cataccgttt tgcccgcgtt
     1261 gggaggtcgt gcggtcgctg acgttacctt cgtgatgtcc acggaaggtc gcaacttcga
     1321 taatctccgg gccaaacatc acatgagcca gacggaaacg gcgacccacc aggcggcagt
     1381 tacggaacag tttacgtacc tgttcaggcg tggcgttagt ggttacgtca aaatctttcg
     1441 gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg
     1501 ctttattgag cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag
     1561 catgctgctc acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc
     1621 tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc
     1681 tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga
     1741 cgcatttgag aatgttgaat ttacaattgc cgactcaggc acggcggtaa gccgccagtt
     1801 tttgacggct gactgaagga tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg
     1861 ctgacccaga aaatgaagtg ccgcgattaa caccgggcgc ggatcgcctt tcggcaacgc
     1921 aggcgcatga ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggtaaatg
     1981 aatgtaatct ggcactttcc agccaaaaag ctggtacagc gagatttgcc gcactgtcgg
     2041 ttcgatcaaa tctgccccgc ggacaatttc gctcacgccc tggaagtgat catccaccac
     2101 caccgccagg ttataggcaa acaaaccatc acggcgatga ataataaaat cttcccgtgc
     2161 cagtttttcg tcggcgtgaa taatgcctcg cagcaggtca gtaaactggg tgaccggatg
     2221 ctgctggcgg atacgtactg cggcgttgtc tggtccatga tgtaattccc ggcaatgacc
     2281 gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgtg caggtgcagt aataacttaa
     2341 tccttgctca tgtaaccagg cgagtgcttc gcgataggcg tggtgacgtt gcgattgcca
     2401 gagaacatcg ccgtcccagt gcaggccgta atgttccagc tggcgcagga tagtatctgc
     2461 ggcaccggga acttcacgag gcgggtcgat atcttcaatg cgtaccagcc agcgaccttg
     2521 cctagcgcga gcctgtaaat agctgccaag cgcggcaatc agagagccaa aatgaagctc
     2581 gccggaagga gagggggcaa agcggccaat atagtgtgtg tctgtcatct ctttgaacaa
     2641 aaaataaggc gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc
     2701 agccatctgt ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt
     2761 cgggcgcgct tccagacggc gaataccaat ttcaacaccg caggattcgc agtagccgaa
     2821 atcttcgtct tccacttttt tcagcgtttt ctcgatcttt ttgatcagct tacgctcgcg
     2881 atcgcggtta cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc
     2941 cgggaagttg gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag
     3001 ttgattacgc catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata
     3061 ctcttcgccc ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga
     3121 cgatgtttta cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc
     3181 cccatgttcg ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat
     3241 tatctaaacg taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga
     3301 atgtaaatta accagttact tactctacta caatgtaacc ggtaatgatt ttttaagagc
     3361 catgccttcg gcagaaagtt ccgctttgta agccagaatt tctacccccc tctgttgagc
     3421 ttctgacaat agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat
     3481 ggctgaatgc agcacggcaa aaaagataac cgcacgctgg ccttcagccg ctacgctcat
     3541 caactcccga aggtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc
     3601 gttttccgct aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg
     3661 caacataaag tcaatacggc tgcgttctgc gccgtattta acttcgcttt tcagcgagct
     3721 atagcctgac agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc
     3781 ccaaagcgta ttgacgcaaa taattgcgcc gctctggctt tgagtcaatt cccaggtgtg
     3841 tgggtatttc cgtttggtgt tgtctgaagt cgaataccag acggtatcgc caggcgttgc
     3901 acaaccggtc atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt
     3961 gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg
     4021 agaaaattcc attacgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg
     4081 tagcgtgtgc gtccacgcgc aaacgaggag gcgtaaaggg tgaactccgt caccgtatac
     4141 gaccagttaa aacctggcgg cgggattgtc accgcctcgc tggcgtcgcg caataaggta
     4201 atatgtggat gaaacggacg attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg
     4261 agcatattcg ccagctggat taagccgcgt ggtggctgac gcatccctaa ccacaccaca
     4321 cgcgaacgca gccattgacc ggcgtcatca agcgtgagtg tgaaacccgg ttgacgaatc
     4381 cgcccggcta aaagagaaag cgccttctct ttctcggcgc tcacttcgcc taaaaatgcc
     4441 agagtcagat gcaaattatc ggcggcgacc ggacgtcccg cgtcaggtgg gaagtgtgtg
     4501 gcgcgccagt ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacagc
     4561 cgttgcggtt cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtgcc
     4621 gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta
     4681 cctgaattac ttaccgctct cgattgtgca tcgcaggtgt tattaagtgc gccgaccggg
     4741 gccgggaaat caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa
     4801 attatccttc tggagccgcg tcgtctggcg gcgcgtaacg ttgcgcaacg actggcggag
     4861 ctgcttaacg aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc
     4921 gggccgaata cccgcctgga agtggttacc gaaggcgtgc tgacgcgcat gatccaacgt
     4981 gacccggaac tgagcggtgt tggattggtg atcctcgatg aatttcatga gcgtagcttg
     5041 caggcggatt tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt
     5101 aaactgctga ttatgtcggc tacgctggac aacgaccgct tgcagcaaat gctgccagaa
     5161 gcgcccgtca tcatctcaga agggcgctcg tttccggttg aacgccgtta tttaccactg
     5221 cccacgcatc agcgttttga cgaagccgtt gcggtagcca ccgccgaaat gctgcgtcag
     5281 gaaagcggat cattactgtt atttttacct ggcgtcggag aaattcagcg tgtgcaggaa
     5341 caactggctt cgcgcatcgg cagtgatgtg ttgctctgcc cgctgtatgg cgcgttgtcg
     5401 ctgaacgatc agcgaaaagc gatccttccg gcaccgcaag gaatgcgcaa agtggtgctg
     5461 gcgaccaata ttgctgaaac aagtttaacc atcgaaggta ttcgtctggt ggtggattgt
     5521 gcccaggagc gtgtggcgcg ttttgatccg cgcacggggc ttacgcggtt gattactcaa
     5581 cgcgttagtc aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt
     5641 atctgcctgc atttaatcgc caaagaacaa gcagaacgcg ctgcggcgca aagtgaaccg
     5701 gagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc
     5761 gatccggcgc agatgagctg gctggatcaa ccgccagcag tgaatttact ggccgcgaaa
     5821 cgcctgttac ggatgctggg ggcgctggac ggtgaacggc ttagtgcgca ggggcaaaaa
     5881 atggcggcgc tgggtaacga tccgcgttta gcagcaatgg tggtgagagc gaagagcgac
     5941 gacgaagctg ctaccgctgc aaaaattgcc gctattctcg aagagccgcc acgggtgggc
     6001 aattgtgacc tgggcgtggc gttttcgcgc aatcaacccg cctggcagca acgtagtcag
     6061 caactgttaa aacgtttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg
     6121 ctacttgccg gggcgtatgc cgatcgcatt gctcgtcgcc gtgggcaaga tggacgctat
     6181 caactggcga acggcatggg ggcgatgctc gatgccgacg atgcgctaag ccgccacgaa
     6241 tggttgattg caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg
     6301 gcgctgccgg tcgatattga tgaattagta caacgctgtc cgcagctggt acagcagtcg
     6361 gacaccgtgg aatgggatga cgcgcaaggc acgctgaaag cctggcgtcg gctgcaaatc
     6421 ggtcagttga cggtgaaagt gcagccgctg gcgaaaccct cggaagacga gttgcatcag
     6481 gcgatgctta acggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg
     6541 gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgactggcca
     6601 gcggttgatg atgagagttt gttggcgacg ctggaaacgt ggctactacc gcatatgagc
     6661 ggcgtgcatt cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta
     6721 cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg
     6781 acgggaagcc ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga
     6841 atgcaggaga tgtttggtga ggccaccaat ccgacgatcg ctcaggggcg cgtgccgctg
     6901 gtgctggagt tgctttcccc tgcccaaagg ccgctgcaaa tcatgcgtga tttgagcgcc
     6961 ttctggaaag gagcgtaccg tgaggtgcaa aaagagatga aagggcgtta tcccaaacat
     7021 gtctggccgg acgacccggc aaacacagca ccaacgcgac gaacgaaaaa gtattcgtga
     7081 agatcaaagt aaatttgaga gatatcttct tctgtcttgc aacagaagaa cagaaaatcg
     7141 ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg cgagccaatt
     7201 ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg tcgttacgaa
     7261 gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa
     7321 ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctgctg
     7381 ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta tctcgatcaa
     7441 aaaattcgca gccgtattga tggtaaggtc tggcaactgc ccgcggcagt ttatggccga
     7501 atggtcaacc ttgagccaga catgaccatc agcaagaacg agatggtgaa gctgctggag
     7561 gcaacacagt atcgtcaggt gtcgaaaatg acccgtcccg gcgaatttac cgtgcaggcc
     7621 aacagcattg agatgattcg ccgtccgttt gatttcccgg acagtaaaga agggcaggtg
     7681 cgtgcgcgtc tgacctttga tggcgatcat ctggcgacga tcgtcaatat ggagaacaac
     7741 cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc ctcgccaaac
     7801 ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt ggatactttg
     7861 ctggcgacag aagaccgcca tttctacgag catgatggga tcagtctcta ctccatcgga
     7921 cgtgcggtgc tggcaaacct gaccgccgga cgcacggtgc agggtgcgag tacgctgacg
     7981 caacagctgg tgaaaaacct gttcctctcc agcgagcgct cttactggcg taaagcgaac
     8041 gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg
     8101 tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg
     8161 gcgagcttgt attactttgg tcgtccggta gaagaattga gcctcgatca gcaggcgctg
     8221 ttggtgggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg
     8281 ctggagcgac gtaacctggt gctacgtttg ctgcaacagc aacagattat tgatcaagaa
     8341 ctctatgaca tgttgagtgc acgtccgctg ggtgttcagc cgcgcggtgg ggtgatctct
     8401 cctcagccag cctttatgca actggtgcgt caggagctgc aggcaaaact gggcgataag
     8461 gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg
     8521 gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa gttgagcgat
     8581 cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc gatggtcggt
     8641 ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt
     8701 tctcttgcaa aaccagcgac ttatctgacg gccttaagcc agccgaaaat ctatcgcctg
     8761 aacacgtgga ttgcggatgc gccaatcgcg ctacgtcagc cgaatggcca ggtctggtca
     8821 ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt ggatgcgttg
     8881 acccgttcga tgaacgtgcc gacggtgaat ctggggatgg cgctggggct gcctgcggtt
     8941 acggagacct ggattaaact gggcgtaccg aaagatcagc tgcatccggt tccggcaatg
     9001 ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca gaccatcgcc
     9061 agcggtggta accgtgcacc gctttctgcg ctgcgttcag tcatcgcaga agatggcaag
     9121 gtgctgtatc agagcttccc gcaggcagaa cgcgcggttc cggcgcaggc ggcatatctg
     9181 acactatgga ccatgcagca ggtggtacaa cgtggtacgg ggcgtcagct tggggcgaaa
     9241 tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt agatacctgg
     9301 tttgcgggca ttgacggcag cacggtgacc atcacctggg tcggccgtga taacaaccag
     9361 ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta tctggctaac
     9421 cagacgccaa cgccgctaaa tcttgttccg ccagaagata ttgcagatat gggcgtggac
     9481 tacgacggca actttgtttg cagcggtggt atgcgtgtct tgccggtctg gaccagcgat
     9541 ccgcaatcgc tgtgccagca gagtgagatg cagcagcagc cgtcaggcaa tccgtttgat
     9601 cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga gcagaaagac
     9661 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaacg tctaagcgtg
     9721 acataccgga tggcgagtag ccatccggta aaataacatc ccacccaaag tattaaccct
     9781 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
     9841 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
     9901 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
     9961 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10021 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10081 gaaagcgcat gggggccagc tgcaactatt gcggcgcgac agtcagctac cggcactaaa
    10141 accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc cgaagagatg
    10201 gcgctgcatc agccgaagtc ggtaaaagaa gctcttagct acactcctgg cgttgccgtg
    10261 ggaactcgtg gcgcatccaa cacttacgat tacctgatca tccgcggatt cgccgctgac
    10321 ggccaaagcc agaacaacta tctgaatggc ctgaagatgc agggcaactt ctataacgat
    10381 gcagttatcg atccgtatat gctggagcgc gccgaaatca tgcgtggtcc ggtatccgtg
    10441 ctgtacggga aaagcagccc tggcggcctg ttgaacatgg tcagcaagcg cccgactaca
    10501 gaaccgctga aagaaattca gtttaaagcc ggtactgaca gcctgttcca gactggtttt
    10561 gatttcagtg atgcgctgga tgatgacggc gtttactctt atcgtctgac tggtattgcg
    10621 cgttcagcca atgctcagca gaagggggca gaagagcagc gttatgctat tgctccagcg
    10681 ttcacctggc gcccggatga taaaaccaat ttcactttcc tttcttactt ccagaacgaa
    10741 ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc gctgccgaac
    10801 ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta ttcacgtaat
    10861 gagaagatgg tgggctacag cttcgatcac gaatttaacg acacctttac tgtgcgtcag
    10921 aacctgcgct ttgcgcaaaa taaagtctcg caaaagagcg tatatggcta cggcatgtgc
    10981 tcggatccgc tgtataccaa agacgacgat gcactcaagg cgagtccatg tttaagtatc
    11041 ccgcagtcag aatggaatca tacactgact cgtcagtacg ttatcgataa tgagaaatta
    11101 gaaaacttct ctgttgatac tcagttgcaa agtaaattcg caaccggctc ggttgaacac
    11161 actctgctga ccggcgttga ctttatgcgt atgcgtaatg atattgactc ctggttcggt
    11221 tacgccggtt ccgttgcacc gtctgatatc tataatttag accgtagtga ctttgatttt
    11281 ggtgctcacc cggatccgtc gggcccatac cgcgttttgc ttaaacagaa acaaaccggt
    11341 ctgtatgttc aggatcaggc gcagtgggat aaggtgctgg tgactctggg tggtcgctat
    11401 gactgggcgg aacagtcatc tttcaaccgt gattacggta ataaatccga tcgtgatgac
    11461 aaacagttca cctggcgtgg tggcgtaaac tacctgttcg acaacggggt aacgccttac
    11521 ttcagctaca gtgagtcgtt tgagccagca tctttgacag atgcaaacgg tgatctgttt
    11581 gcaccttcga aaggcaaaca gtatgaagtt ggtgtgaaat atgtgccgga agatcgccca
    11641 attgtgctga cgggcgcact gtatcagctt accaaaacca acaacctgat ggcggatccg
    11701 aataatccca atttctcgat tgaaggcggt gagattcgcg ctcgtggtgt agaactggaa
    11761 gcaaaagcgg cgctgtcggc gagtgttaac gtggttggtt cttataccta taccgatgcg
    11821 gaatacacta ccgatactac ctacaaaggc aatacgcctg cacaggtgcc aaaacacatg
    11881 gcttcgctgt gggctgacta taccttcttt gacggtccgc tttcaggtct gacgctgggc
    11941 accggtggtc gttatactgg ctccagctat ggtgatccgg ctaactcctt taaagtggga
    12001 agttatacgg tcgtggatgc gttagtgcgt tatgatctgg cacgagtcgg catggctggc
    12061 tccaacgtgg cgctgcatgt caacaacctg ttcgatcgtg aatacgtcgc cagctgcttt
    12121 aacacctatg gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt
    12181 ttctaatttc tcttttgggg cacggatttc cgtgcccgtt tcacaagttg gctgttatgc
    12241 aggaatacac gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc
    12301 ccgggcgcac gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc
    12361 tgattggtca caacggttct ggtaaatcca ctctgctcaa aatgcttggt cgtcaccagc
    12421 caccgtcgga aggggagatt cttcttgatg cccaaccgct ggaaagctgg agcagcaaag
    12481 cgtttgcccg caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgacgg
    12541 tgcgtgaact ggtggcgatt ggtcgttacc cgtggcatgg cgcactaggg cgctttggtg
    12601 cggcagatcg cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc
    12661 atcggctggt cgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg
    12721 tggcgcagga tagccgttgt ctgttgctcg acgaaccgac ctcggcgctg gatatcgccc
    12781 accaggttga cgtgctggcg ctggtgcatc gtttaagtca ggaacgtggt ctgacggtca
    12841 ttgccgtgct gcacgatatt aatatggcgg cgcgctactg tgattatctg gtcgccctgc
    12901 gcggcggtga aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg
    12961 aaatgattta tggcatcccg atgggtattc tgccgcatcc ggcgggtgct gcacctgtga
    13021 gttttgttta ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat
    13081 ggcgctttct ccgttgttat ggcagatgaa tactgcccac gcggcggcta ttgatcccaa
    13141 tcgtattgtg gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc
    13201 ttacggcgtg gcggatacca tcaactatcg cctgtgggtc agcgaaccac cattgccgga
    13261 ctcagtgatc gacgtcggtt tgcgcacaga acctaacctt gaactgctga ccgaaatgaa
    13321 accatcgttt atggtctggt cggcaggata tggcccttca ccagaaatgc tggcacgtat
    13381 tgcgccgggg cgtggattta acttcagcga cggcaaacag ccgctggcga tggcgcgtaa
    13441 atcactgacg gaaatggcag atttacttaa cctgcaaagc gcagcagaaa cgcatttagc
    13501 gcaatatgaa gactttatcc gcagcatgaa cccccgcttt gtgaagcgtg gtgcacgccc
    13561 gttattgctg acgacgctta tcgatccgcg ccatatgttg gtcttcggtc caaacagctt
    13621 gttccaggaa attcttgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt
    13681 ctggggcagt accgccgtca gtatcgatcg tctggcggcg tataaagacg ttgatgtgct
    13741 ctgttttgat cacgacaaca gcaaagacat ggacgcccta atggcgacgc cgctgtggca
    13801 ggccatgccg tttgtccgcg ccggacgctt tcagcgcgtt ccggcggtct ggttctatgg
    13861 cgcgacgctt tcggcaatgc actttgtacg tattctggat aacgccatcg gaggtaaagc
    13921 gtgagtaaac gaattgcgct tttcccggcg ttattgctgg cgctgctggt gattgtcgca
    13981 gcggcgctca cctggatgaa cttttcgcaa gcgctgccgc gtagccagtg ggcgcaggct
    14041 gcctggtcgc cggatatcga tgtcatcgag cagatgattt ttcactacag cttgctgccg
    14101 cgtctggcga tttcgctgct ggtgggcgcg ggcctggggc tggtgggtgt gctgtttcag
    14161 caagtgctgc gtaacccgct ggcggaaccg acgacacttg gcgttgctac aggcgcgcaa
    14221 ctggggatta ccgtcactac gctctgggcg atccccggcg cgatggcgag ccagtttgct
    14281 gcgctggcag gggcttgtgt ggttggctta attgtctttg gcgtcgcgtg ggggaaacgg
    14341 ctgtcgccgg taacgctgat tctcgcgggg ctggtagtga gcctttattg tggggcaatc
    14401 aatcagttac tggttatctt ccatcatgac cagttgcaaa gcatgttcct gtggagcacc
    14461 ggaacgctga cgcaaaccga ctggggcggc gttgagcgtt tatggccgca gctgctgggc
    14521 ggcgtgatgc tgacgttact gctacttcgt ccgttaaccc tgatggggct tgatgatggc
    14581 gtggcgcgca acctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg
    14641 atagtcatca gtgcgctgct ggtgaacgca gtggggatta tcggctttat cgggttgttc
    14701 gcgccactgc tggcaaaaat gctgggggcg cggcgtttgt tgccacggtt gatgctggcg
    14761 tcgctgattg gtgcgttaat tctgtggctt tctgatcaaa tcatcctctg gctgactcgc
    14821 gtgtggatgg aagtttccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg
    14881 tggctgctgc cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc
    14941 gcggctgaac gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg
    15001 gctgtggtag tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg
    15061 gcgttgctcg aggatttaat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg
    15121 ggcgtcatgc tggcggtggc gggctgtatt attcagcggc tgaccggaaa cccgatggca
    15181 agcccggaag tgctggggat tagctccggc gcggcgtttg gcgtggtgtt gatgctgttt
    15241 ctggtgccgg gtaatgcctt tggctggctg ttacctgcag gcagtctcgg cgcggcggtg
    15301 acgctgttga tcattatgat cgccgccgga cgcggtggat tttccccaca ccgcatgtta
    15361 ctggcgggga tggcgttaag taccgcgttc accatgcttt tgatgatgtt gcaggcaagt
    15421 ggtgacccgc gaatggcgca agttctgacc tggatttccg gttcgaccta caacgcgacc
    15481 gatgcgcagg tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg
    15541 tgccgccgct ggctgaccat tttaccgctg ggaggtgata ccgcacgagc cgtggggatg
    15601 gcgctgacgc cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac agcgactgcg
    15661 acaatgacca ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg
    15721 atgggctttc gacggacgat gccacacatc gtaatttcgg cgctggtggg aggattactg
    15781 ctggtgttcg ctgactggtg tgggcggatg gtgctgtttc cattccagat cccagcgggg
    15841 ctgctgtcga cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt
    15901 taattgaatg cctcgcccgg tgggcgaggc aaaatcatca caacttcgca aacacccgac
    15961 gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacagac ataaagcccg
    16021 cttcaaacgc tgacggtgcc agataaacac cttcgtccag catcatatgg aagaaacgct
    16081 taaagcgttc cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg
    16141 taaagaaaat accgaacatg ccgccaacgt ggtttaccac cagcggaatt ccggcttctt
    16201 ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccagc gtttcgtgaa
    16261 cgcccggctg cgcgacttca ttcagacagg cgaaaccagc agccatcgca atcgggttac
    16321 cggaaagcgt acccgcctga tagaccggac cggtcggggc cagcgcatcc attacatcac
    16381 gacgaccacc gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtga
    16441 gatccggttc cacaccgtaa taatcctgtg cgccagctag cgctacgcgg aaaccggtca
    16501 tcacttcatc gatgatcagc aacgcgccaa attcgtcgca cagcgcgcgc agacctggca
    16561 ggaactctgg cagcggtgga acgcagttca tattgcctgc caccggctcg acgataatac
    16621 aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaggccaga tcgttataag
    16681 tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgaattt ggctggccta
    16741 acgtgagtgc gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac
    16801 cttcaaattt aataattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca
    16861 ttgtcgcttc agtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg
    16921 tcaccagttg cgccattttc acttccattt cggttggtgc accaaagctt aaaccacgct
    16981 cggcggcttc aatcacggca ttgcggatcg ccggatggtt atggcccagc accatcggcc
    17041 cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt
    17101 ccgctttttc gataaacagt ggagtaccgc ccacgccagt aaaggcgcga acaggggagt
    17161 tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg
    17221 agggttcctg attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt
    17281 aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa
    17341 aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa
    17401 aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc
    17461 tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt
    17521 gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc
    17581 gggctggctg cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca gcgtatgggg
    17641 gcgctggtac atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg
    17701 gcggtgctgg cgatgttcgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt
    17761 tcggggatcc cggaaattga aggggcgctg gaagatcaac gtccagttcg ctggtggcgt
    17821 gtgttgccgg tgaaattctt tggcgggctg ggaacactcg gcggaggcat ggtgctgggc
    17881 cgcgaagggc caaccgtaca gatcggcggc aatatcggtc gcatggtgct tgatattttc
    17941 cgcctgaaag gtgatgaagc gcgccatacg ctgctggcaa ccggtgctgc ggcggggctg
    18001 gctgcggcct ttaacgcccc gctggcgggt attttgttta ttatcgaaga gatgcgtccg
    18061 cagtttcgct atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc
    18121 attatgtacc ggatttttaa tcatgaagtt gcattgattg acgtcggtaa actttctgac
    18181 gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc
    18241 cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc
    18301 aatattacca aatgggtact gatgggcggt gcaattggcg gtctgtgtgg attgctgggg
    18361 tttgtggcac cagcaacgtc gggcggcggc tttaacctga ttcctatcgc taccgcaggg
    18421 aatttcagca tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactc
    18481 tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg
    18541 ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca ccttgaggcg
    18601 gggacatttg ctattgccgg aatgggggca ttactggcgg catctattcg cgcgccgtta
    18661 acggggatca ttctggttct ggagatgacc gataactacc agctcatttt gccaatgatt
    18721 attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatactcg
    18781 gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc
    18841 gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta
    18901 ttgggttaga atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta
    18961 gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac
    19021 gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc
    19081 cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag
    19141 ggcgttggcc tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac
    19201 tataccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc
    19261 tgcggttgcg gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc
    19321 ggcacatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat
    19381 gccaacgaat agccgccagt cgaatcaata gcgtcacgac catgcccatc atactggctg
    19441 tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac
    19501 aggcagttgc gtagatttct gtacgtaaaa tcatggggat ctcgcgggcc agtacatcac
    19561 gaatgatccc gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg
    19621 cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt
    19681 ccagcaccgg caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca
    19741 gcatgctggt taccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc
    19801 cgtgatccag cgccatgtcg cgaattgttc cgccgcctac tgcggttacc acgcccagta
    19861 ccagaacacc aaaagggtcc atacgcaatt ttccggctaa caaaacgccg gagatggcaa
    19921 ataccgctgt gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca
    19981 cctgaaaagg tcaggcgcta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg
    20041 aggataatac gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt
    20101 ttgagctgtt caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg
    20161 acaatcgcct gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc
    20221 cggctgtctt taaagatgtt ttctccgcca caaacttcga gtacctggtt ctgaatcgac
    20281 tcttttccac tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt
    20341 ttatcagcat attgcgcttt caattgcgcg tattggtcca gcaaggattg tgctgcttgt
    20401 tcagccttgt ctggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt
    20461 tcaatgcttg tcgcatcgac ccacatcact tttattccca gcgaagccag ctggtcaacc
    20521 tgccgctcgg cattacctcc acgccaggca atcaccagat cgggtttcag cgccacaatg
    20581 cgttccagat tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga
    20641 tagtcggaat agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg
    20701 ttggcgggag aaagcgtgat gacgcgcggc gcggcgttga gccacagcgg cacgagaaaa
    20761 gacagggcga ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga
    20821 ctcaaccatc aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg
    20881 agactgttga tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt
    20941 gtggcagaca tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt
    21001 gtggcggatt ttcgccagac caacagaacc attgatgaaa gcgtcgccgc taacaatcag
    21061 gccacgtaca gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt
    21121 atcgtcagct ttgaagcctg ccgggcagcc tggtaactga ccgtattcat aaccaaatgc
    21181 cgtgacatcc gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcag
    21241 cgttggtgcc aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc
    21301 caacagcaga gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc
    21361 aacctcggtt ccattcagtt ggccggtata gatttcgcaa ccgcccagac tgatagtttg
    21421 acggttttcg attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc
    21481 gattttcata gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta
    21541 cggggaagcg tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa
    21601 taaactggca tcgtcgttac cgttcaccac agggcgttaa aaccgaacat gagatcctgc
    21661 gtatcttcga gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac
    21721 aaaagaccca ggtttttcca ctggagcgca atgctgccgt gcgcacgcgt cttacccact
    21781 cgatggaagt ccagcaggtg gggcgctaca tcgccaaaga aattttaagc cgtctgaaag
    21841 agcttaaatt gctggaagca tacggcctgg aagaactgac cggccccttt gaaagcattg
    21901 ttgagatgtc atgccttatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag
    21961 cggcgataaa tgactggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc
    22021 tgactgacga tcgctgcagc gtggcagcac tacgtttacg ggacggggaa gaaccgctta
    22081 acgagctgcg gcgcaaaata cgtcaggact tatgtcattt tgaggggaat gcacaaggca
    22141 ttcgtctggt gcatacattg atgcggatga atctcacctg ggcacaggtt ggcggtattt
    22201 taaaatatac tcgtccggcg tggtggcgtg gtgaaacgcc tgagacacat cactatttaa
    22261 tgaaaaagcc gggttattat ctttctgagg aagcctatat tgcccggttg cgtaaagaac
    22321 ttaatttggc gctttacagt cgttttccat taacgtggat tatggaagct gccgacgaca
    22381 tctcctattg tgtggcagat cttgaagatg cggtagagaa aagaatattt accgttgagc
    22441 agctttatca tcatttgcac gaggcgtggg gccagcatga gaaaggttcg ctcttttcgc
    22501 tggtggttga aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag
    22561 atcagttttt tatgtattta cgggtaaaca ccctaaataa actggtaccc tacgcggcac
    22621 aacgatttat tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg
    22681 aagatgccag cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg
    22741 tgtttagcca tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat
    22801 tattagagat ttatcgtcct ttattaagcc tgtcgttatc agactttact gaactggtag
    22861 aaaaagaacg ggtgaaacgt ttccctattg aatcgcgctt attcaacaaa ctctcgacgc
    22921 gccatcggct ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc
    22981 cgctatggga atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg
    23041 acctctatgc ctgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt
    23101 gtaaagacga acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta
    23161 taaaacgaat ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca
    23221 tgaaaaaaac cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc
    23281 cgctctctgc aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc
    23341 ttgcaccgat gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca
    23401 caaccgttaa tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc
    23461 cgttctgcca ggaaggttct ccgttccaga gctctccgtt ctgccagggg ggccagggcg
    23521 gtaatggcgg cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg
    23581 ccgataaagg ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag
    23641 tccagctgag cgatggtcgt aagttcgacg cgaagatggt tggcaaagat ccgcgctctg
    23701 atatcgcgct gatccagatc cagaacccga aaaacctgac cgcaattaag atggcggatt
    23761 ctgatgcact gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg
    23821 agacggtaac ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact
    23881 acgaaaactt catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg
    23941 ttaacctgaa cggtgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca
    24001 acatcggtat cggttttgct atcccgagca acatggtgaa aaacctgacc tcgcagatgg
    24061 tggaatacgg ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactctg
    24121 aactggcgaa agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc
    24181 ctaactcctc cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaatg
    24241 gtaagccgat cagcagcttt gccgcactgc gtgctcaggt gggtaccatg ccggtgggca
    24301 gcaaactgac cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc
    24361 agcagagcag ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg
    24421 agatgagcaa caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc
    24481 cggctgcgca gatcggcctg aaaaaaggtg atgtgattat tggcgcgaac cagcaggcag
    24541 tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga
    24601 acattcagcg cggcgacagc accatctacc tgttaatgca gtaatctccc tcaacccctt
    24661 cctgaatacg ggaaggggtt ctccttacaa tctgtgaact tcgccacaac tccatacatc
    24721 ttcatcatcc tttgggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc
    24781 gtgcataatg gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc
    24841 gtggcacgta ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgcgggcga
    24901 attatcggca gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta
    24961 ctttcacagg gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg
    25021 cggcaaggga ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg
    25081 actggtgaac cagagaacct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg
    25141 atgctggaac agtcgcggtt gatgcacctg ttggcgcagg atagccgttt gcgggaagaa
    25201 ctggtgatga acctgattca ggctgaggag aatacccccg cacttactga atgggcgcaa
    25261 cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt
    25321 cagcttggcg tggacagcgc aatggcggag ttacagcaac tgcaaaacgc gctgactacg
    25381 cctgagcgta ataatctggt ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg
    25441 gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg
    25501 attacccgca tgaaagagta cggccagctg cgttttcgcg tttcgctggg taactatttt
    25561 accggaccgg gcagtattgc ccgctcttat cgtacggcga aaacgacgat ggtggtgggt
    25621 aaacagcgaa tgccagaaag tcgctgctat ttttatcagg atctgatgtt gcctgtgtta
    25681 ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg
    25741 aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat
    25801 gtgcaaccgc tggctacgtc aaaggcgctg tttattcatc gtaatactct ggagtatcgg
    25861 cttaatcgta tatcggaact gaccgggctg gatttgggca attttgatga caggttgctg
    25921 ctgtatgtgg cgttgcaact ggatgaagag cggtaggtcg atgacaaaat tacagccggg
    25981 tgagacttta cccggcccaa agaatggcat tatttacgcg tcagtttttc cagatcggct
    26041 tcaatctcgc tgatcttatt ggttactact gactccagat gacgtaagtc gtcgaggatc
    26101 ttacgcttaa gatcaacttc gctgcggtca cgctggcaga tttgatctaa ctcatcaatg
    26161 atatagcgaa gattagggct aatctcctgc acttctttat aaccctgacc cacaccatcg
    26221 gcgacgaccg ttttacgctg acgcggatat ttaaacttca cgctcttggc gaaaaactcg
    26281 cctttgtctt tctggaaata gattttcaga atatcgttgt tggcttcctg ccggaggctg
    26341 taacgatcaa tttcttcagg attggtaata cccagacttt tcagattgtc gtacatagcg
    26401 ttacctcaaa atgagtcagt aaattgtgct tattttagca tttggcctgg cccgcccggc
    26461 tcgactgtga tcgcagaaag cctttttctt ccagacttct atcatggcgt aaaaatcaaa
    26521 aaattacctg ctttattgtg gcgatattcc atgggatgga ttagcacatt tatctttgtt
    26581 ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta tttaaaaaag gggaagaaga
    26641 atgagtagga cgaattattc tgaaccagaa agcaatcggg cagcgtcgtg ctgcccgtgt
    26701 gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc
    26761 gcgtcaactt tcttaacgat aaccgcacag taaagactgt atttgccatc tttcgacggc
    26821 agattgcctg aaacaaccac agatcctgcc ggaacgcgac cgtagtggat ttcgccggtt
    26881 tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatagagat gacggaacct
    26941 tcttcgacaa tcaccccttc aaccacttca gagcgcgcgc cgatgaagca gttatcttca
    27001 ataatggtcg ggttagcctg cagcggctcc agcacgccgc cgatacccac gccaccggaa
    27061 aggtggacgt ttttaccaat ctgcgcacag gaaccgacgg tcgcccaggt atcaaccatc
    27121 gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg
    27181 gcaatgaacg cgccctgacg cactgccgct ggtggcacga cgcggaagcc ttctttctgg
    27241 aaacgtgctt catcgtagtt agcgaatttc atcggcactt tgtcgaagta gcggctttct
    27301 gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac
    27361 cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc gccggaatcc
    27421 agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcatt ggctggcgtg
    27481 atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa
    27541 ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac
    27601 cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt
    27661 atgaataaat cttctactcg ttcgccaatg gttgtaattc gggcgccatg aagcgaaatt
    27721 cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggttggtc gagcgcgata
    27781 agctcaagga acgatttgcg gtcggtatgg gtcggcaaaa atgtcacttc agtttcaaca
    27841 gtaaagtgac gtagtttggc cggttggcga cgtggctgcg gtggctgcca gctactttgt
    27901 gtcagtacct gctccagacc aaatcgaata acttcatggc gatctgcgga cagcgggctg
    27961 ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt
    28021 tgtgcgtcgt ggacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga
    28081 taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcggacta
    28141 agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgcgcatg ccaggccagt
    28201 tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc
    28261 gcctcttcgt cgatgttatc catgcgcagt agtgccagcg cctggagttg gtgatggcga
    28321 acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggcg
    28381 aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg
    28441 catatatcgg ccactgtcag gcataccaga taacgaagtc ggttttctgt ttgcacttct
    28501 tcggcaaact gcttgatcac ttccgggtcc tgaatatcgc ggcgctgagc tgttaccgac
    28561 atcagcaagt gctggcgaac cagccaggcg accagttgcg tctcgcgcga gttcaaccca
    28621 tggagttcgg caaaatgcac tacatcctga gcaccgagaa tagagtggtc gccgccgcgt
    28681 cctttggcaa tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc
    28741 ggccagacgt ccacgcacaa cggatggcgt tggcgcgttt cttcactggc aaaactctcc
    28801 agtttcagca tcacgcggat agtatgttca tccaccgtat aggcgtggaa caggtcaaac
    28861 tgcatttgcc cgacgatatg cgaccattgc ggcatatacg cgccgagtac gctatggcga
    28921 tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt
    28981 tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgcaga
    29041 tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac
    29101 atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc
    29161 gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg
    29221 aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta
    29281 acgcggaagt aatccttcat catccgctcg accggttcgt taccttcacc actgtaattc
    29341 aaacgctggg cgacgctaag ctggcgatca aacagcaggc gattatcgta acggctgacg
    29401 accagatgca gggcaaagcg aatacgccac aatatatgca gacattcgtt taattccgct
    29461 cgctccgctg aggttaagaa gccaaacccg accatttcat ccagcgatgt tgcgccaaaa
    29521 tgacggcggg ccacccattg cagcgtgtgg atatcgcgca agccgccagg gctacttttg
    29581 atgtctggct caaggttgta gctggtgccg tggtaacgct gatggcgctg gttctgttct
    29641 tcaactttcg ctgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt
    29701 tgcagttcga ggaagagcgc tacatcgcca attaataagc gggattcgat taaattggtg
    29761 gcgacggtta aatccgataa tccttccagc atgcactctt caagcgtgcg cacgctatga
    29821 ccgacttcca gcttcacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga
    29881 tcatccggga gctttttacg gcttaaaatc agtaaatcga tgtcggaaag tggatgcagc
    29941 tcgccacgac cataaccacc gacggcaacc aatgccaggt cggcaatctg gctgaatccc
    30001 gcttcaatcc ataatcgttg caggagctgg tctatgaact cggtacgtgc ctcaatcaac
    30061 tgttctgcgg agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata
    30121 tgggctttta tcccacagac ggttaattca tcacggggcc aggcacatgg attttgcggt
    30181 tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg
    30241 cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta
    30301 ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca
    30361 gccgttatca gtcaccacaa tagtatgctc gtattgtgca gacaagctgc gatctttggt
    30421 ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg
    30481 ctcgatggtg aaggtcatcc ccggtttcag cacgacgttg gtttcacggg agtcatagtg
    30541 cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccacaat attcacgaac
    30601 gacggagaag ccttctgctt cgacaaactt ctgaattgcc gcaccgattt cgcgcagatt
    30661 aatgccaggt tttaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag
    30721 acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa
    30781 accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttggc
    30841 gtcgtccggg ataccgtggc acaccacttc attaatagaa atgcaaacgg atttcggata
    30901 gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca
    30961 gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag
    31021 cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat
    31081 tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg
    31141 ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc
    31201 actgaaggtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt
    31261 attggtgtcc gcgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat
    31321 acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca
    31381 tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt
    31441 ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt
    31501 tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc
    31561 gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct
    31621 ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg
    31681 cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca
    31741 tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct
    31801 gaaagacctg gaaactcagt ctcaggacgg tactttcgac aagctgacca agaaagaagc
    31861 gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat
    31921 gggcggtctg ccggacgctc tgtttgtaat cgatgctgac catgaacaca ttgctatcaa
    31981 agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga
    32041 cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct
    32101 gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga
    32161 agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt
    32221 gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga
    32281 gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca
    32341 ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt
    32401 gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca
    32461 tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca
    32521 ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct
    32581 attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc
    32641 gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac
    32701 gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact
    32761 gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt
    32821 ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat
    32881 cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt
    32941 aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc ggaagatgta
    33001 tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt
    33061 aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa
    33121 gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg
    33181 aaagagcata acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag
    33241 aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatcg
    33301 aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct
    33361 tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt
    33421 gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc
    33481 ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac
    33541 tggatcgcat ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg
    33601 tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg
    33661 ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg
    33721 cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt
    33781 gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc
    33841 tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta
    33901 tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg
    33961 atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc gaagtgctcg
    34021 aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac
    34081 cgattcgtgt tttcaacatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa
    34141 aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc
    34201 gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg cagtttggtc
    34261 acggccagcg cgcagcaacc ggagcgtaca caagtgcgtg aggatggcga gcactgcccg
    34321 gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa
    34381 tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt
    34441 tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca
    34501 aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca
    34561 gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg cgtcagctgg
    34621 caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt
    34681 ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg
    34741 gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca
    34801 aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg
    34861 cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc
    34921 gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc
    34981 tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct
    35041 cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga
    35101 tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg
    35161 gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat
    35221 gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat
    35281 gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc
    35341 ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg
    35401 gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa
    35461 accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc
    35521 gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag
    35581 agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg
    35641 gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg
    35701 gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg acgtaaaatt
    35761 tccgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggctg
    35821 tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca
    35881 atggtgcgct accaggacgg cagtgttctg gcgcagctgg gtgaaccgga tatgcgtacg
    35941 ccaattgctc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccactcgat
    36001 ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg
    36061 aaactggcga tggaggcgtt cgaacaaggc caggcagcga cgacagcatt gaatgccgca
    36121 aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgccgcg
    36181 ttgaatttat ccgtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg
    36241 ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc
    36301 tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt cagtgatata
    36361 gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttac
    36421 acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg tcagggaata
    36481 agaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca gcgcatggct
    36541 gtcgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag caagggaaga
    36601 ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg
    36661 ccaacaacgg tattgaggcg ttaactctgt atgcctttag tagtgaaaac tggaaccgac
    36721 cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgcttgat agcgaagtaa
    36781 agagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt cgctttaact
    36841 cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agccgggaat accggtctga
    36901 cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga gtcaggcaac
    36961 tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa gagatgctaa
    37021 accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg actggggggg
    37081 agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt tactttacag
    37141 atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat gcctttgcta
    37201 atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga tgggggtcgc
    37261 ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt
    37321 tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct ggtggtctgc atgctggcag
    37381 cgtgggaatg gggacagctt agcggtttta ccactcgctc gcagcgagta tggttggcgg
    37441 tgttatgtgg gttattgttg gcgctgatgc tttttctgtt gccggaatat caccgaaata
    37501 ttcatcaacc gctggttgaa atctcacttt gggcttcgct gggttggtgg agtgtcgcgc
    37561 tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct aaaacattgc
    37621 gcattatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg gcgttacggg
    37681 cctggcacta cgacgagaat cattacagtg gcgcaatatg gctgctctat gtcatgatcc
    37741 tggtatgggg cgctgactct ggcgcatata tgtttggcaa attgtttggt aaacataaac
    37801 tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga ctcgcgactg
    37861 cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct cccgtcacct
    37921 tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg accgagagta
    37981 tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga cacggtggta
    38041 ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc ttgttgttac
    38101 tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg atttggcttc
    38161 gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt tctgggttgc
    38221 ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg cgctctggcg
    38281 gcgaactgat aagctcggca ccgaatatgt tatcgccctg atcccgctgg gcggttatgt
    38341 caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc atgccttcaa
    38401 taataaatct gtcggccaac gagcggcgat tattgccgca ggtccggttg caaacttcat
    38461 ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg tacgtccggt
    38521 ggttggcgaa atagcagcca attcgatagc tgcagaagca caaattgcac caggtacgga
    38581 actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt
    38641 cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca gcgaccaacg
    38701 gcgggatgta aagctcgatt tacgtcactg gacgtttgag cctgataaag aagatccgat
    38761 aacttcgctg gggattcgtc ctcgtgggcc gcaaattgaa tctgtactgg aaaatgtgca
    38821 gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga
    38881 tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata acccgggtaa
    38941 atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat taatcccgga
    39001 gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga aagtcattcc
    39061 tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc
    39121 cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa aattgatcac
    39181 cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg gggctgggat
    39241 gacagcggaa ctcggggttg tttattacct gccgtttctt gcgcttatta gcgtgaactt
    39301 agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc tgttccttgc
    39361 gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt gttatcgcat
    39421 tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt tctctcggtt
    39481 atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt gctcatagcg
    39541 tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt gaaagatatt
    39601 catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgc
    39661 acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct gtttgctacc
    39721 ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt tcaggtaaaa
    39781 gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa agatgacatg
    39841 ctgaaacaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc
    39901 attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa atatagcgcc
    39961 agcgttaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc
    40021 caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca tgctttcacc
    40081 accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg gaacgtggta
    40141 ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct gcgcagctac
    40201 tatctggacc gcggttatgc ccgtttcaac atcgactcta cccaggtcag tctgacgcca
    40261 gataaaaaag gtatttacgt cacggtgaac atcaccgaag gcgatcagta caagctttct
    40321 ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca gctgactaag
    40381 atcgagccgg gcgagctgta taacggcacc aaagtgacca aaatggaaga tgacattaaa
    40441 aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac
    40501 gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg tttctacgtg
    40561 cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg
    40621 cgtcagatgg aaggtgcatg gctggggagc gatctggtcg atcagggtaa ggagcgtctg
    40681 aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc gggtagcccg
    40741 gaccaggttg atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt caacttcggg
    40801 attggttacg gtactgaaag tggcgtgagc ttccaggctg gtgtgcaaca ggataactgg
    40861 ttaggtacgg gttatgctgt tggtatcaac gggaccaaaa acgattacca gacctatgct
    40921 gaactgtcgg taaccaaccc gtacttcacc gtagatggcg taagcctcgg tggtcgtctc
    40981 ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa caagagttat
    41041 ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg tgcaggtctg
    41101 ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg
    41161 tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa aacggacgac
    41221 ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt cccgacagat
    41281 ggttcacgtg tcaacctgac tggtaaagtg accattccgg gatcggataa cgaatactac
    41341 aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa atgggttgtt
    41401 ctgggtcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat gccgttctac
    41461 gagaacttct atgccggtgg ttccagcacc gtccgtggct tccagtccaa taccattggt
    41521 ccgaaagcag tttacttccc gcatcaggct agtaattatg atccggacta tgattacgaa
    41581 tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac
    41641 gctatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag cgataagtat
    41701 gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga tacaaactgg
    41761 gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat ccgtatgtct
    41821 gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc ctacgcccag
    41881 ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat cggtaaaacc
    41941 tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa
    42001 tataagatcg ccgggacacg caaagaactg caccctccgg tgcaaatggg atggtaagga
    42061 gtttattgtg aaaaagtggt tattagctgc aggtcttggt ttagcactgg caacttctgc
    42121 tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc aggtagcgca
    42181 gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca
    42241 gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga aagcgggcag
    42301 cgatcgcacc aaactggaaa aagacgtgat ggctcagcgc cagacttttg ctcagaaagc
    42361 gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca aactggttac
    42421 tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc tggttgttga
    42481 tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg acgtactgaa
    42541 acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc agttggatgc
    42601 agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc aatctgcaca
    42661 aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag gcttgtgcca
    42721 ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt
    42781 agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata ccacgccgca
    42841 gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa
    42901 cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg ataacgtgat
    42961 tatcggtgcc ggttgcttcg taggtaaaaa cagcagaatc ggtgcaggtt cgcgtctctg
    43021 ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga tccagtccgg
    43081 aacagtggta ggtgcagacg gctttggtta tgccaacgat cgtggtaact gggtgaagat
    43141 cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggcgcat gtactaccat
    43201 cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg ataaccagtg
    43261 ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg gcgtcattat
    43321 ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg taatcaacgg
    43381 gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga tgcgtcccat
    43441 cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa
    43501 aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat cgcttgagcg
    43561 caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta cggcctgtct
    43621 cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt tgacaggaag
    43681 agtatcttga ctactaacac tcatactctg cagattgaag agattttaga acttctgccg
    43741 caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg
    43801 cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt ccctggaaaa
    43861 ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg tattctggcg
    43921 tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg tattgacgaa
    43981 gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt cactttcgaa
    44041 aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgacgg taaagtagtt
    44101 tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat tgataaatcc
    44161 gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt cgattggcgc taacgctcac
    44221 attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac cgtactgaaa
    44281 tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat ttatcagttc
    44341 gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac ccgcgtggaa
    44401 atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac agtccagggc
    44461 ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca cattgcgcac
    44521 gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc gggtcacgta
    44581 tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt ctgcatcatt
    44641 ggtgcgcatg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc tccttatgtc
    44701 attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc
    44761 ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat ttatcgtagc
    44821 ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac atatccggaa
    44881 gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg
    44941 aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat atcctggggg
    45001 ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc ccgctttgtt ggtgttgccg
    45061 ggccacgaat gcaggctgaa ggctgcgaag cctggtacga aatggaagaa ctggcggtga
    45121 tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt gccgatctga
    45181 caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg cccgacttca
    45241 atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc
    45301 catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc accgatctgg
    45361 tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct
    45421 ttatcggtca taccatggct gatgccatgc cattagatcc agataaaaat ggtgcccgtg
    45481 atgtgctggg gatcccttac gatgcccact gtctggcttt gttgccgggc agccgtggtg
    45541 cagaagtcga aatgcttagt gctgatttcc tgaaaacggc tcagcttttg cgccagacat
    45601 atccggatct cgaaatcgtg gtgccgctgg tgaatgccaa acgccgcgag cagtttgaac
    45661 gcatcaaagc tgaagttgcg ccagacctgt cggttcatat gttggatggg atgggccgtg
    45721 aggcgatggt cgctagtgat gcggcactac tggcgtcggg gacggcagcc ctggagtgca
    45781 tgctggcgaa atgcccgatg gtggtggggt atcgcatgaa gcctttcact ttctggttgg
    45841 cgaagcgtct ggtgaaaact gattatgtct cgctgccaaa tttgctggcg ggcagagagt
    45901 tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa actggctgcg gcgctgttac
    45961 cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt gaactgcatc
    46021 agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag ttagcacaat
    46081 gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag tcggacgcgg
    46141 gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccatcgc gcccgattgc
    46201 cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct gtgaagagat
    46261 caaagataaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa tcgacgaact
    46321 gaatatttta cacgcgacca tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc
    46381 gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc cttcgatggc
    46441 agtggtgaaa ggcgatagcc gcgtaccaga aatcagtgct gcgtctatcc tggcgaaagt
    46501 gacgcgcgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg gttttgccca
    46561 acacaaaggg tatccaactg cttttcatct ggaaaaactg gctgaatatg gcgcgactga
    46621 acatcatcgt cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt
    46681 gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg
    46741 ggtgcacagc gactactcga tgatcgatgg tctggccaaa accgcaccgc tggtaaaaaa
    46801 ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc tttgcggtct
    46861 ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg gggcagattt
    46921 taatgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtgc tggcggcgaa
    46981 caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc gcgggtacgg
    47041 tgcagctggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct
    47101 tctttccggc gggcgcatgg gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct
    47161 ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt ttctcgagct
    47221 gatccgcacc ggcaggccgg atgaagaaag ctatctgcac gcggcggtgg aactggcgga
    47281 agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc tttatcgaca gcagcgactt
    47341 tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg atcctaaacg
    47401 cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt gcgagctgtt
    47461 tgccgacatc ccagaagccc ttgccaacac cgttgagatc gccaaacgct gtaacgtaac
    47521 tgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga gcaccgaaga
    47581 ttatctggtc atgcgtgcaa aagagggcct ggaagagcgt ctggcatttt tattccctga
    47641 cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga ctgaacttca
    47701 ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat ttatccagtg
    47761 gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg gttcactggt
    47821 ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc tgttcgaacg
    47881 tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct gtatggagaa
    47941 acgcgaccag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg tatcgcagat
    48001 tatcaccttc ggtacgatgg cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg
    48061 gcatccgtac ggctttgtcg accgtatctc gaaactgatc ccgcccgatc cggggatgac
    48121 gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag cggatgaaga
    48181 agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta acgccggtaa
    48241 gcacgccggt ggggtggtta tcgcaccgac caaaattacc gattttgcgc cgctttactg
    48301 cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg aatacgccgg
    48361 actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact gggcgctgga
    48421 gatgatcaac aagcggcggg cgaagaatgg cgagccgcct ctggatatcg ccgcgatccc
    48481 gctggatgac aagaaaagct tcgacatgct gcaacgctcg gaaaccactg cggtattcca
    48541 gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact gcttcgaaga
    48601 tatgatcgca ctggtggcac tgttccgccc cggtccgttg caatcaggga tggtggataa
    48661 ctttatcgac cgtaaacatg gtcgcgaaga gatctcctat ccggacgtgc agtggcagca
    48721 tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc aggaacaggt
    48781 catgcagatt gcccaggtgc tttctggtta taccctcggt ggcgcggata tgctgcgtcg
    48841 tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat ttgctgaagg
    48901 tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc tggtggagaa
    48961 attcgctggt tacggattta acaaatcgca ctctgcggcc tatgctttgg tgtcatatca
    49021 aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa tgaccgccga
    49081 tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga tggggctgaa
    49141 aatcctgccg ccggatataa actccggtct ttaccatttc cacgtcaacg acgacggcga
    49201 aatcgtgtat ggtattggcg cgatcaaagg ggtcggtgaa ggtccgattg aggcgatcat
    49261 cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg cccgaaccga
    49321 caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg cgtttgaccg
    49381 tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggc gatgcgttaa aagcggcaga
    49441 tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggtgtgc tggccgaaga
    49501 gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc aggtggtatt
    49561 agatggggaa cgtgaaacgt taggtctgta cctgacggga caccctatca atcagtattt
    49621 aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg
    49681 tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg
    49741 cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt
    49801 gtttactgac gccctggata aataccagca attgctggaa aaagaccgca tacttatcgt
    49861 cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt
    49921 gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct cgctgacgga
    49981 caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaac cccaccgctc
    50041 tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc ggttgcgttt
    50101 tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc gtggcctcat
    50161 tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga gtctgaattt
    50221 ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc tgactgcggt
    50281 tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc gtctgcgtga
    50341 aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc agattgcgca
    50401 actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg catttgatga
    50461 atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg tcggtggtat
    50521 cgcccgtctc gatggtcgtc cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa
    50581 agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca aagcactgcg
    50641 tctgatgcaa atggctgaac gctttaagat gccaatcatc acctttatcg acaccccggg
    50701 ggcttatccg ggcgtgggcg cagaagagcg cggtcagtct gaagccattg cacgcaacct
    50761 gcgtgaaatg tctcgcctta gcgtaccggt agtttgtacg gttatcggtg aaggtggttc
    50821 tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat acagcaccta
    50881 ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg acaaagcgcc
    50941 gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga aactgatcga
    51001 ctccatcatc ccggaaccgc tgggtggtgc tcaccgtaac ccggaagcga tggcggcgtc
    51061 tctgaaagcg cagctgctgg cggatctggc cgatctcgac gtgttaagca ctgaagattt
    51121 aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg caaatgttct
    51181 gaaaagggtc acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag
    51241 gaaggatttt ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct
    51301 tttataaaga tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga
    51361 ttatctggcc acaaaacagc gttgatttgc tgaaatttat tgaacataac cctcgaattt
    51421 gcggcgtgat ttttgactgg gatgagtaca gtctcgattt atgtagcgat atcaatcagc
    51481 ttaatgaata tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg
    51541 tgcaggatat gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcagaagata
    51601 tcgccattcg tatgcgtcag tacaccgacg aatatcttga taacattaca ccgccgttca
    51661 cgaaagcctt gtttacctac gtcaaagagc ggaagtacac cttttgtacg ccgggtcata
    51721 tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct gttttatgat tttttcggcg
    51781 ggaatactct taaggctgat gtctctattt cggttaccga gcttggttcg ttgctcgacc
    51841 acaccgggcc acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga
    51901 gttatatcgt taccaacgga acatcgacgt ctaacaaaat tgtgggtatg tacgccgcgc
    51961 cgtccggcag tacgctgttg atcgaccgca attgtcataa atcgctggcg catctgttga
    52021 tgatgaacga tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg
    52081 gtgggatccc gcgccgtgaa tttactcgcg acagcatcga agagaaagtc gctgctacca
    52141 cgcaagctca atggccggtt catgcggtga tcaccaactc cacctatgat ggcttgctct
    52201 acaacaccga ctggatcaaa cagacgctgg atgtcccgtc gattcacttc gattctgcct
    52261 gggtgccgta cacccatttt catccgatct accagggtaa aagtggtatg agcggcgagc
    52321 gtgttgcggg aaaagtgatc ttcgaaacgc agtcgaccca caaaatgctg gcggcgttat
    52381 cgcaggcttc gctgatccac attaaaggcg agtatgacga agaggcgttt aacgaagcct
    52441 ttatgatgca taccaccacc tcgcccagtt atcccattgt tgcttcggtt gagacggcgg
    52501 cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc
    52561 tgcattttcg caaagaggtc cagcggctgc gggaagagtc tgacggttgg tttttcgata
    52621 tctggcaacc gccgcaggtg gatgaagccg aatgctggcc cgttgcgcct ggcgaacagt
    52681 ggcacggctt taacgatgcg gatgccgatc atatgtttct cgatccggtt aaagtcacta
    52741 ttttgacacc ggggatggac gagcagggca atatgagcga ggaggggatc ccggcggcgc
    52801 tggtagcaaa attcctcgac gaacgaggga tcgtagtaga gaaaaccggt ccttataacc
    52861 tgctgtttct ctttagtatt ggaatcgata aaaccaaagc aatgggatta ttgcgtgggt
    52921 tgacggaatt taagcgctct tacgatctca acctgcggat caaaaatatg ctacccgatc
    52981 tctatgcaga agatcccgat ttctaccgca atatgcgcat tcaggatctg gcgcaaggga
    53041 tccataagct gattcgtaaa cacgatcttc ccggtttgat gttgcgggca ttcgatacct
    53101 tgccggagat gatcatgacg ccacatcagg catggcagag gcagattaaa ggcgaagtag
    53161 aaaccattgc gctggaacaa ctggttggta gggtgtcggc aaatatgatc ctgccttatc
    53221 caccgggcgt accgctgctg atgccaggag aaatgctgac caaagagagc cgcacagtac
    53281 tcgattttct actgatgctt tgttccgtag ggcaacatta ccccggtttt gaaacggata
    53341 ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt acgagtccta aaaatggcgg
    53401 gataacttgc cagagcggct tccgggcgag taacgtgctg ttaacaaata aaggagacgt
    53461 tatgctgggt ttaaaacagg ttcaccatat tgcgattatt gcgacggatt atgcggtgag
    53521 caaagcgttt tactgcgata ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc
    53581 gcgcgactcg tggaaagggg atttggcgct taatgggcaa tatgtgattg agcttttctc
    53641 atttccgttc ccgccggagc gccccagccg accggaagct tgcggcctgc gtcatctggc
    53701 ttttagcgtt gatgatatcg atgcggcagt ggcgcacctt gaaagccata acgtgaagtg
    53761 tgaagccatc cgtgtcgatc catacacgca aaaacgcttc acgttcttta acgatccgga
    53821 cgggctgccg ttggaactgt atgagcagta aggcttgtca tcgccgcatt tgcccggtaa
    53881 cgtgccgggc attgctactg taaaatcgca ccatcatgac actcacgctc aatagacaac
    53941 ttctcacctc acgccagatt ctggtggcct ttagcggcgg gcttgactct accgtactgc
    54001 tgcatcagtt ggtgcagtgg cgggcggaaa atccgggcgt cactctgcgc gctatccatg
    54061 tgcatcacgg tttaagtgcc aatgccgatg cctgggttag gcattgcgag aacatctgcc
    54121 aacagtggca ggtgccgctg gtggtcgagc gcgtgcaact tgcgcaagaa ggactgggta
    54181 ttgaggccca ggcgcggcag gcgcgttatc aggcatttgc ccgcaccttg ttgcccggtg
    54241 aagtgctggt caccgcgcaa catctcgacg atcaatgcga aacctttctg ctggcgctaa
    54301 aacgcggtag cggccctgcc gggctttcgg ctatggggga agtttcggag tttgccggaa
    54361 cacggcttat ccgcccgttg ctcgcccgca cgcggggaga actggtgcag tgggcgcgtc
    54421 agtatgattt acgctggatt gaagacgaaa gtaatcagga tgatagctac gatcgtaact
    54481 tcctgcgcct gcgcgtggtg ccgttattgc agcagcgttg gccgcatttt gccgaagcaa
    54541 cggcccgcag cgctgcgctt tgcgctgaac aagagagcct gttagatgaa ctgctggcgg
    54601 atgatatagc gcactgtcaa aagccgcagg ggacgctgca gattgcgcca atgctggcga
    54661 tgagtgatgc ccgccgcgcg gcgattatcc gccgctggct ggcagggaag aatgcaccga
    54721 tgccttcccg cgacgcgttg gtgaggatct ggcaggaagt ggcgctggcg cgggaagatg
    54781 cttccccctg tttacgtctg ggcgcgtttg aaatccgccg ctatcagtcg caactgtggt
    54841 ggattaaatc cgttgccggg caaagcgaaa ccattgtgcc gtggcagacg tggctacaac
    54901 cgctggaatt accggcggga ctgggaagtg tacagcttac agcgggaggc gatattcgcc
    54961 ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc gccaggattg ctgcatatta
    55021 tcgggcgcaa cggcggacgt aagctgaaga aaatctggca agagctgggt gtgccgccgt
    55081 ggctacgtga caccacgcca ctgctgtttt atggcgaaac gttgattgcg gcggcagggg
    55141 tatttgtgac gcaagaaggt gtggctgaag gtgagaaagg cgtcagtttt gtctggcaga
    55201 aaacgcttag ttaagtgaaa gccggataag acgcttcaaa cgtcgcatcc ggcgaaagtc
    55261 aatcaggact cgctcaccac caccgtaccg atttccgggt ggctaaagct ggtaatttta
    55321 tccagacgca gctcgcgggt tgcaccagcg gcctcgacga ccagatactc cacatttttg
    55381 cgggagacta aatcgctggc cttcgcctgc aatttttcgc catctttcag ctcaagtgtc
    55441 agcattaaat gatgctggca ggcgagctca agattatcgt aatcatcaca attgattggt
    55501 tgatacgtat cattcattga cataatcgct caccagtaag tttgccgcag cgtatgctgc
    55561 tttttccctg acagcctcag aaagggcgtc gtcggcagcc atttcattca gcactttcaa
    55621 aacgcagccc agcgcgtccg gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg
    55681 cttgcgtatt aactcacaat atttttccac atgccctcct gtcagcactc tgacttaacc
    55741 gtggatgcaa gtctaagcct acgaagataa actctgtttc gcaaggtgac tataccacac
    55801 tcatttctgc aatatcagcg ccgcaactgc acgtattccg ttacaatggc ctcctgattc
    55861 aaaaggagtt ttcttatggc gcttaaagcg acaatttata aagcgacggt taatgtggcc
    55921 gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa
    55981 acccaggagc gcatgatgct gcgcctgctg gcgtggctga aatatgctga tgaacgtctg
    56041 caatttaccc gtggtttgtg tgccgatgat gagccggaag cgtggctgcg taacgatcac
    56101 ctgggcattg atttgtggat tgagctgggg ctgccggatg agcggcggat taagaaagcc
    56161 tgcacccagg ccgcagaagt tgcgctgttt acctataata gtcgggcggc gcaaatctgg
    56221 tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc tttccatctg gtatctggac
    56281 gatgaacaac tggcgaaagt aagcgccttt gccgatcgta ccatgacgct gcaggcaacg
    56341 attcaggatg gcgtgatctg gttatcggat gataagaata atctggaagt gaacttaacc
    56401 gtctggcaac aaccttcatg attgtgattt cccgacatgt tgctatcccc gatggtgagc
    56461 ttgagatcac cgccattcgt gcgcagggcg cgggcgggca gcatgttaat aagacctcaa
    56521 cggctattca tctgcgtttt gacattcggg cgtccagcct gccagagtat tacaaagagc
    56581 gtctgctcgc cgccagccat catttgatca gcagtgatgg cgtgattgtc attaaggcac
    56641 aggaataccg caatcaggag ctcaaccgcg aagcggcgct ggcccggctg gtagcagtga
    56701 ttaaagattt aacaacagaa caaaaagccc gacgacccac gcggcccacc cgcgcatcaa
    56761 aagagcgcag actggcatcg aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag
    56821 tgcgcagcgg tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg
    56881 atggctgtaa tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg
    56941 ctttctccgg cgcaggctgc cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg
    57001 ccgtgtgccg attgcgaagg gatcgaaacc tctttgttcc tcgaaaaaga tggaacctgg
    57061 gtgatgaatg aacgctatct tggtgcgcgt gaagagcctt cctctttcgc ttcctacggt
    57121 acatgggcgc gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat
    57181 tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg aaggtaatcc gattgaatcg
    57241 cagttcaact atacgctgga accggcgcaa tccagtttac ctatgacgcc gatgaccctg
    57301 cggggcatgt atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa
    57361 cgtttcatgg tagcgaataa cgcagagctg gagcgtggct acctgactgc gcgcggtcat
    57421 agtgaaaaac cgatgttact gtcattagaa gggcacttta cgcttgaggc taatccggat
    57481 accggtgcgc caaccaaagt attagcgccc gatacggcag gtaaatttta cccgaaccag
    57541 gattgcagta gtttggggca gtaaattgaa agccggagca ttctccggct ttcatcactg
    57601 agtcagccca acaaagcggc ccgccttgat ataaatccca ggcttcaggt gcccaacata
    57661 ctgtaactgc gtttctggct taaacgcaga gacatcgcca gtacgcatat gcatcagatc
    57721 ggcagggcta atccagcccg attgcgccag tgtaagtgga tgcccggctt tggcaaatgc
    57781 atcagtgaca aattccgaac aaaaccacga ctttttgtct ccttcgccca cactgcttaa
    57841 ctgcgctttc gccaggccgc tgacgcactg ttgacgaaaa tcctcggaga acggattcag
    57901 tgagcacatc tggcgagtta ccataaaggg aataaattcg acaatgccgc gatagttgta
    57961 accgctatct ttaattttgt tggcgaacgc ggtgatttct gtggcttgtt gcggggtaag
    58021 atccgggact cgtaggacga aaagcttatc gctatgcttc atcgcttttt tgagggaaac
    58081 aatctggacg ccagcgcctg tcgcttctgc aacgttattt tcacccagat agattgcaac
    58141 gtgactcaca gaggaagtgc tgaagacgcg gattccaaat gaggttaccc catggcttga
    58201 ggagaacagc aaatcgccgg gtttgagatc aggttccgtt atttctttta ttgattgttc
    58261 ggtaaaagag ctttgatgct ggaatttgac tgcccatgtt ttcgccccgg catctaccgc
    58321 tgtggctgat gagtccggct ggctgatatc tacggtacag gccgaaagga aaagaaagca
    58381 ggggagaagc aggcggcaat gcacctttgg tttatccatt ttatacagtc catgtaaaaa
    58441 agggccctga aattcaggac cctttctggc atcagccttt aatctgtttc accagatatt
    58501 cgacgatgtc accagtctta attaactgtt tctcgccgtt gcgacgatat ttatattcga
    58561 tatcgtcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt
    58621 ccatatcagc aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac
    58681 cttgtgcacg cagttcgctg tacagtttct cggccagctc ctgtacgcgg aaggatttgt
    58741 gcatgttcat cggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc
    58801 cgcgttcgtc atagttctgc tcaattgccg cagccacaac gcgcgttacc ccgataccgt
    58861 agcaacccat cgtcaggatt tgattacggc catcttcacc ctgaacggag gctttcagtg
    58921 cttcggagta cttggtaccc agctggaaga tgtgaccgac ttcgatacca cgtttgatca
    58981 gcagcgtacc ctgaccatcc gggcttggat cgccagccac cacgttgcgg atatcagcaa
    59041 tttctggggt agcgacatcg cgatcccagt tgataccgaa gtagtgttta ccatcgatgt
    59101 tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc actggaatcg
    59161 gcatgtttac cggacccagt gaacccggac cggctttaac cacggcacga atttcttctt
    59221 cggtcgcgaa agtcagcggg cttgcaacct gcggcagttt ttctgcttta acttcgttca
    59281 gctcgtgatc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaaccgctt
    59341 taaccagcag agtcttcacc gttttctcaa tcggcagatt gaactgttca accagttccg
    59401 cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg
    59461 gttctttcgg cgcgatagct tctgccagct caatgttcgc tgcatagtca gaggtgtcgg
    59521 agaagaccac atcgtcttca ccgctctgcg ccagcacctg gaattcgtga gaggcgctac
    59581 cgccgataga accggtgtcg gcctgtacgg cgcggaaatc cagccccatg cggctgaaga
    59641 ttttgctgta ggccgcatac attgcatcgt aggtttcctg taaggattcc tgagaagtat
    59701 ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgccg aaacgcggac
    59761 gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag
    59821 agctcagctc gttacgaatc aggtctgtaa taacttcttc atgagttggg ccgagtacga
    59881 acggacgctc gccacggtca acaaaacgca gcagttccgg accgtactgt tcccaacgac
    59941 cactctcttg ccacaaatcg gctggctgaa ccaccggcat cgacacctcg atcgcaccgg
    60001 cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg
    60061 gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct
    60121 gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc
    60181 tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg
    60241 acaaaaaaag tgatttagtt taccagtgca aaagaaatgt caaaagagaa gggcgcgaat
    60301 ttaacgcggt tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt
    60361 aaaatcatgc agccagacgg cataggtttt gcccgtttcc tcacctttac gataggccgg
    60421 gcgcgggtcc tgcgccagta cttcgcggat aaacagcgtt aactgcggat aacgtttctc
    60481 cagcgtcaaa agctgctttt cgacctctgc ggtaaagctc accgccatct ctgccgctgg
    60541 cgcactttgc gcatagctgg cactggcatt tggaagcgat tcggcaaagg ggagatacgg
    60601 tttgatatcc accaccggcg taccatctac cagatccaga ctgccgagct tcagaatcac
    60661 gctgtcttta tggcaaacaa cctctttcag ctctaccagc gacataccaa tcgggttagg
    60721 gcggaaagtg gagcgcgtag cgaaaacacc cattctggca ttaccgccga ggcgcggtgg
    60781 acgcacagtc ggacgccagc cgccttccat cgtttgatga aagacgaaaa ggatccataa
    60841 atggctgaac gcttccaggc cgcgaacggc atcggcctgg ttgtagggag caatgagatg
    60901 cagttcgccg ttggcgcttt ttaccagacc tggctggcgc ggaacggcga atttttcttt
    60961 atagggcgag cgaataacgc ctatttgctc gaactggaaa ctgctcattt cgccgtaatg
    61021 ttaagcgcag aaccgataca tacagcctga cgatagcagc ctggcgtacc gctggtgact
    61081 tcgcagctat gcagtaatac cgcattggcc ttcattttcg aggcgttgat ttgcatccgc
    61141 ttacgtgcgg ttggaatgct cggcggagag tcctgattgg aggcctggca agaatcgcca
    61201 ctgacttcac cgagatcgcg gaacggtttg ccgactaatt cttctgcatt ggtataaatt
    61261 cggaccggcg tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg ctggggtgca
    61321 gtgctttgaa cgggttcgac aggggatctg cttaacatgg aacagccgct tagcatgagt
    61381 gctactaaac agatcggtaa agcacgcata gtatttcctc aatgtatgat caaaacgtca
    61441 atattgaatc aggagcttgt aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat
    61501 tacaaattgt ggaaacagcc taaaaattac cagcctttaa cagcgccgcc gttaaacact
    61561 ttgtttgctg cttcgtaaac ttcgtcagac tgataagcct ggacgaattt cttcacgttc
    61621 tcggcgtctt tgttatcttc acgcgtcacg atcaggttta cgtacgggga gtctttatct
    61681 tcgacaaaga taccgtcttt cgccggagtc aggccaatct ggctggcata ggtggtattg
    61741 ataactgcca gagcgatttg cgcgtcgtcc agagagcgag gcagctgcgg tgcttccagc
    61801 tcaacaattt tcagattttt tgggttctca acaacatcaa gaacggtcgg cagcaggcca
    61861 acgccatctt tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaagg
    61921 ttagttgggt cgtttggcac ggcaacctgc gaaccatcct gcaattcatc cagtgatttg
    61981 attttcttgg agtaaccagc aatcggataa acaaaagtgt tgcctactgc gaccagtttg
    62041 tagccacgat ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg
    62101 atatcgcctt tgctcaatgc ctcgtttggc agaacatagt cgttgaaggt taccagctca
    62161 acgtccagac catatttgtc tttcgcaact ttctgcgcga cttctgcaac ctgctgttcg
    62221 gcaccaacaa tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct
    62281 accaatgcca gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc
    62341 ataccttatt ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc
    62401 cggacgatgc ggtcgcctgc gaactgaatt aaataaacca gaatgaccag caataccagt
    62461 accgtattca tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct
    62521 aaaccaccgg caccgactgc accacccatc gcggaataac cgaccagggt aataagggtg
    62581 atagttgccg cattcaccag acccggcaac gcttccggta acagcacttt acggacgatc
    62641 tgcattggcg tggcacccat tgcgcgggaa gcttcaatta acccggttgg gatctccagc
    62701 agagcgttct cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatc
    62761 gctgcctgca aaccaatcga tgtaccgaca ataacgcggg taaacggaat catccatacc
    62821 agcaagataa tgaacgggat agaacggaaa atgttcacaa tcgcagaaac ggtacgatac
    62881 agcttcgcgt tagcaataat ttgccccggg cgcgtaacat aaagcaggac gccgaccggc
    62941 agaccaatca caaacccaaa aaatccggat acgaaggtca ttgccagcgt ttcccatacg
    63001 ccacgaacca gcagccacat catcggctca gacataaccc agtacctcta cttttacatg
    63061 gtgttcctgc agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt
    63121 cagcatgatg ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt
    63181 gttgacgttg aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt
    63241 aaactccaga cgcagcatcg gcacgcagtc agtgaatggc tccgcttgca gacgctcctg
    63301 ataatcttcc gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt
    63361 tttcggatgc gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattactgat
    63421 aaccgccacg caatcacaaa tgcgcttcac aacgtccatt tcgtgggtga tcaacagaat
    63481 cgtcaaaccc agacggcggt taatgtcttt caacagttcg agaatagaac gtgtcgttgc
    63541 cgggtccagc gcgctggtgg cttcatcaca cagcaatact ttgggattgc tggctaatgc
    63601 acgggcaatc gccacacgtt gtttctgccc accggaaaga ttcgacgggt agctatcatg
    63661 cttatcgcca agaccaacca atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt
    63721 cggtgtattg tccagctcca gcggcagagc cacgttgcca aaaacagtac gcgaagagag
    63781 caggttaaaa tgctggaaaa tcataccaat ctggcggcga gctttggtca actcggattc
    63841 tgacagcgtg gtcagttcct gtccatcgac cagcacgcta ccctcggttg ggcgctccag
    63901 caggtttaca caacgtataa gcgtactctt acccgcgcct gaggcaccga taacgccata
    63961 aatttgtcca gctggcacat gcaggctgac gttgttcaat gcctggatgg tgcgggtgcc
    64021 ctggtggaac actttggtga tattcgaaag ttttatcatt gattatttat tatcgtcatt
    64081 aagttagtcg tggcatttcg aatgcctgga acgggcaacg ccgtcaatga aatggatgtt
    64141 aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag agcactttct tgctggtcga
    64201 aacatgcgat actagcgtca catgccttat taaggagcta taaaggtggc gaagagcgta
    64261 cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta tgtccatgag
    64321 atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg
    64381 ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa atttactgaa
    64441 gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg cgatgtcgat
    64501 ctggatggta tctattattg cccgcatcat ccgcagggta gtgttgaaga gtttcgccag
    64561 gtctgcgact gccgcaaacc acatccgggg atgcttttat cggcacgcga ttatttgcat
    64621 attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca ggcagcggct
    64681 gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctgttac gcctgaagca
    64741 gaaaacgcgg cggattgggt gttaaatagc ctggcagacc tgccgcaagc gataaaaaag
    64801 cagcaaaaac cggcgtaatg attaaaagat gagcggttga aataaaaatg catttttccg
    64861 cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg atgtgaatca
    64921 cttcacacaa acagccggtt cggttgaaga gaaaaatcct gaaattcagg gttgactctg
    64981 aaagaggaaa gcgtaatata cgccacctcg cgacagc
//
LOCUS       DARNVM010000005        63862 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.005, whole genome shotgun sequence.
ACCESSION   DARNVM010000005 DARNVM010000000
VERSION     DARNVM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.005"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            33..482
                     /gene="alaE"
                     /locus_tag="SI621_000293"
     CDS             33..482
                     /gene="alaE"
                     /locus_tag="SI621_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HEI4063055.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(519..863)
                     /locus_tag="SI621_000294"
     CDS             complement(519..863)
                     /locus_tag="SI621_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HEI4063056.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1015..1344
                     /locus_tag="SI621_000295"
     CDS             1015..1344
                     /locus_tag="SI621_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEI4063057.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1401..2735)
                     /locus_tag="SI621_000296"
     CDS             complement(1401..2735)
                     /locus_tag="SI621_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEI4063058.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEVALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVLPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            2823..3254
                     /locus_tag="SI621_000297"
     CDS             2823..3254
                     /locus_tag="SI621_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEI4063059.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3463..3708
                     /gene="nrdH"
                     /locus_tag="SI621_000298"
     CDS             3463..3708
                     /gene="nrdH"
                     /locus_tag="SI621_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEI4063060.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3705..4115
                     /gene="nrdI"
                     /locus_tag="SI621_000299"
     CDS             3705..4115
                     /gene="nrdI"
                     /locus_tag="SI621_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEI4063061.1"
                     /translation="MSQLVYFSSSSENTQRFIERLRLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRHPQNA"
     gene            4088..6232
                     /gene="nrdE"
                     /locus_tag="SI621_000300"
     CDS             4088..6232
                     /gene="nrdE"
                     /locus_tag="SI621_000300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEI4063062.1"
                     /translation="MATTPAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     ITTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYAEL
                     VANERIRKKYINTRDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFAHTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERSETFAGFKQSRYANGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6242..7201
                     /gene="nrdF"
                     /locus_tag="SI621_000301"
     CDS             6242..7201
                     /gene="nrdF"
                     /locus_tag="SI621_000301"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEI4063063.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPT
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7558..8760
                     /gene="proV"
                     /locus_tag="SI621_000302"
     CDS             7558..8760
                     /gene="proV"
                     /locus_tag="SI621_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HEI4063064.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8753..9817
                     /gene="proW"
                     /locus_tag="SI621_000303"
     CDS             8753..9817
                     /gene="proW"
                     /locus_tag="SI621_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HEI4063065.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9875..10867
                     /gene="proX"
                     /locus_tag="SI621_000304"
     CDS             9875..10867
                     /gene="proX"
                     /locus_tag="SI621_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HEI4063066.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11320..12504
                     /locus_tag="SI621_000305"
     CDS             11320..12504
                     /locus_tag="SI621_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063067.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMI
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12628..13365
                     /gene="ygaZ"
                     /locus_tag="SI621_000306"
     CDS             12628..13365
                     /gene="ygaZ"
                     /locus_tag="SI621_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HEI4063068.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLKSVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13355..13690
                     /gene="ygaH"
                     /locus_tag="SI621_000307"
     CDS             13355..13690
                     /gene="ygaH"
                     /locus_tag="SI621_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HEI4063069.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13781..14311
                     /gene="emrR"
                     /locus_tag="SI621_000308"
     CDS             13781..14311
                     /gene="emrR"
                     /locus_tag="SI621_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HEI4063070.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14438..15610
                     /gene="emrA"
                     /locus_tag="SI621_000309"
     CDS             14438..15610
                     /gene="emrA"
                     /locus_tag="SI621_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HEI4063071.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15627..17165
                     /gene="emrB"
                     /locus_tag="SI621_000310"
     CDS             15627..17165
                     /gene="emrB"
                     /locus_tag="SI621_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HEI4063072.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17229..17744)
                     /gene="luxS"
                     /locus_tag="SI621_000311"
     CDS             complement(17229..17744)
                     /gene="luxS"
                     /locus_tag="SI621_000311"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEI4063073.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17894..19450)
                     /gene="gshA"
                     /locus_tag="SI621_000312"
     CDS             complement(17894..19450)
                     /gene="gshA"
                     /locus_tag="SI621_000312"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEI4063074.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDIAGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSKELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19523..19951)
                     /locus_tag="SI621_000313"
     CDS             complement(19523..19951)
                     /locus_tag="SI621_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4063075.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19948..20514)
                     /gene="yqaB"
                     /locus_tag="SI621_000314"
     CDS             complement(19948..20514)
                     /gene="yqaB"
                     /locus_tag="SI621_000314"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HEI4063076.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20662..20738)
                     /locus_tag="SI621_000315"
     tRNA            complement(20662..20738)
                     /locus_tag="SI621_000315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20702..20704),aa:Arg,seq:acg)
     gene            complement(20841..20917)
                     /locus_tag="SI621_000316"
     tRNA            complement(20841..20917)
                     /locus_tag="SI621_000316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20881..20883),aa:Arg,seq:acg)
     gene            complement(20980..21056)
                     /locus_tag="SI621_000317"
     tRNA            complement(20980..21056)
                     /locus_tag="SI621_000317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21020..21022),aa:Arg,seq:acg)
     gene            complement(21255..21331)
                     /locus_tag="SI621_000318"
     tRNA            complement(21255..21331)
                     /locus_tag="SI621_000318"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21295..21297),aa:Arg,seq:acg)
     gene            complement(21335..21427)
                     /locus_tag="SI621_000319"
     tRNA            complement(21335..21427)
                     /locus_tag="SI621_000319"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21391..21393),aa:Ser,seq:gct)
     gene            complement(21744..21929)
                     /gene="csrA"
                     /locus_tag="SI621_000320"
     CDS             complement(21744..21929)
                     /gene="csrA"
                     /locus_tag="SI621_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEI4063077.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22164..24794)
                     /gene="alaS"
                     /locus_tag="SI621_000321"
     CDS             complement(22164..24794)
                     /gene="alaS"
                     /locus_tag="SI621_000321"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEI4063078.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24922..25422)
                     /gene="recX"
                     /locus_tag="SI621_000322"
     CDS             complement(24922..25422)
                     /gene="recX"
                     /locus_tag="SI621_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEI4063079.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25491..26552)
                     /gene="recA"
                     /locus_tag="SI621_000323"
     CDS             complement(25491..26552)
                     /gene="recA"
                     /locus_tag="SI621_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEI4063080.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26632..27129)
                     /gene="pncC"
                     /locus_tag="SI621_000324"
     CDS             complement(26632..27129)
                     /gene="pncC"
                     /locus_tag="SI621_000324"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HEI4063081.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27274..28359)
                     /gene="mltB"
                     /locus_tag="SI621_000325"
     CDS             complement(27274..28359)
                     /gene="mltB"
                     /locus_tag="SI621_000325"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEI4063082.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28615..29178
                     /gene="srlA"
                     /locus_tag="SI621_000326"
                     /gene_synonym="gutA"
     CDS             28615..29178
                     /gene="srlA"
                     /locus_tag="SI621_000326"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEI4063083.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            29175..30134
                     /gene="srlE"
                     /locus_tag="SI621_000327"
                     /gene_synonym="gutE"
     CDS             29175..30134
                     /gene="srlE"
                     /locus_tag="SI621_000327"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEI4063084.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            30145..30516
                     /gene="srlB"
                     /locus_tag="SI621_000328"
                     /gene_synonym="gutB"
     CDS             30145..30516
                     /gene="srlB"
                     /locus_tag="SI621_000328"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEI4063085.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30520..31299
                     /gene="srlD"
                     /locus_tag="SI621_000329"
     CDS             30520..31299
                     /gene="srlD"
                     /locus_tag="SI621_000329"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HEI4063086.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31405..31764
                     /gene="gutM"
                     /locus_tag="SI621_000330"
     CDS             31405..31764
                     /gene="gutM"
                     /locus_tag="SI621_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HEI4063087.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31831..32604
                     /gene="srlR"
                     /locus_tag="SI621_000331"
                     /gene_synonym="gutR"
     CDS             31831..32604
                     /gene="srlR"
                     /locus_tag="SI621_000331"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HEI4063088.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            32597..33562
                     /gene="gutQ"
                     /locus_tag="SI621_000332"
     CDS             32597..33562
                     /gene="gutQ"
                     /locus_tag="SI621_000332"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HEI4063089.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33559..35073)
                     /gene="norR"
                     /locus_tag="SI621_000333"
     CDS             complement(33559..35073)
                     /gene="norR"
                     /locus_tag="SI621_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HEI4063090.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35260..36699
                     /gene="norV"
                     /locus_tag="SI621_000334"
     CDS             35260..36699
                     /gene="norV"
                     /locus_tag="SI621_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HEI4063091.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSANTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36696..37829
                     /gene="norW"
                     /locus_tag="SI621_000335"
     CDS             36696..37829
                     /gene="norW"
                     /locus_tag="SI621_000335"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HEI4063092.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFTEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEIGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCTE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(37907..40147)
                     /gene="hypF"
                     /locus_tag="SI621_000336"
     CDS             complement(37907..40147)
                     /gene="hypF"
                     /locus_tag="SI621_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEI4063093.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAASLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TTSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RILHAA"
     gene            complement(40300..40827)
                     /gene="hydN"
                     /locus_tag="SI621_000337"
     CDS             complement(40300..40827)
                     /gene="hydN"
                     /locus_tag="SI621_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HEI4063094.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40976..41986)
                     /gene="ascG"
                     /locus_tag="SI621_000338"
     CDS             complement(40976..41986)
                     /gene="ascG"
                     /locus_tag="SI621_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HEI4063095.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SQ"
     gene            42246..43703
                     /gene="ascF"
                     /locus_tag="SI621_000339"
     CDS             42246..43703
                     /gene="ascF"
                     /locus_tag="SI621_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HEI4063096.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            43712..45136
                     /gene="ascB"
                     /locus_tag="SI621_000340"
     CDS             43712..45136
                     /gene="ascB"
                     /locus_tag="SI621_000340"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HEI4063097.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARICFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDVGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(45215..45634)
                     /locus_tag="SI621_000341"
     CDS             complement(45215..45634)
                     /locus_tag="SI621_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4063098.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(45631..45942)
                     /locus_tag="SI621_000342"
     CDS             complement(45631..45942)
                     /locus_tag="SI621_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEI4063099.1"
                     /translation="MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(46109..46579)
                     /gene="hycI"
                     /locus_tag="SI621_000343"
     CDS             complement(46109..46579)
                     /gene="hycI"
                     /locus_tag="SI621_000343"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HEI4063100.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(46572..46982)
                     /gene="hycH"
                     /locus_tag="SI621_000344"
     CDS             complement(46572..46982)
                     /gene="hycH"
                     /locus_tag="SI621_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HEI4063101.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46979..47746)
                     /gene="hycG"
                     /locus_tag="SI621_000345"
     CDS             complement(46979..47746)
                     /gene="hycG"
                     /locus_tag="SI621_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HEI4063102.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47746..48288)
                     /gene="hycF"
                     /locus_tag="SI621_000346"
     CDS             complement(47746..48288)
                     /gene="hycF"
                     /locus_tag="SI621_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HEI4063103.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(48298..50007)
                     /gene="hycE"
                     /locus_tag="SI621_000347"
     CDS             complement(48298..50007)
                     /gene="hycE"
                     /locus_tag="SI621_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HEI4063104.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(50025..50948)
                     /gene="hycD"
                     /locus_tag="SI621_000348"
     CDS             complement(50025..50948)
                     /gene="hycD"
                     /locus_tag="SI621_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HEI4063105.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50951..52777)
                     /gene="hycC"
                     /locus_tag="SI621_000349"
     CDS             complement(50951..52777)
                     /gene="hycC"
                     /locus_tag="SI621_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HEI4063106.1"
                     /translation="MSAISLINSGVAWFAAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATSAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52774..53385)
                     /gene="hycB"
                     /locus_tag="SI621_000350"
     CDS             complement(52774..53385)
                     /gene="hycB"
                     /locus_tag="SI621_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HEI4063107.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(53510..53971)
                     /gene="hycA"
                     /locus_tag="SI621_000351"
     CDS             complement(53510..53971)
                     /gene="hycA"
                     /locus_tag="SI621_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HEI4063108.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            54183..54533
                     /gene="hypA"
                     /locus_tag="SI621_000352"
     CDS             54183..54533
                     /gene="hypA"
                     /locus_tag="SI621_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEI4063109.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            54537..55409
                     /gene="hypB"
                     /locus_tag="SI621_000353"
     CDS             54537..55409
                     /gene="hypB"
                     /locus_tag="SI621_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEI4063110.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            55400..55672
                     /gene="hypC"
                     /locus_tag="SI621_000354"
     CDS             55400..55672
                     /gene="hypC"
                     /locus_tag="SI621_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HEI4063111.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55672..56793
                     /gene="hypD"
                     /locus_tag="SI621_000355"
     CDS             55672..56793
                     /gene="hypD"
                     /locus_tag="SI621_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEI4063112.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56790..57800
                     /gene="hypE"
                     /locus_tag="SI621_000356"
     CDS             56790..57800
                     /gene="hypE"
                     /locus_tag="SI621_000356"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HEI4063113.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57874..59952
                     /gene="flhA"
                     /locus_tag="SI621_000357"
     CDS             57874..59952
                     /gene="flhA"
                     /locus_tag="SI621_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HEI4063114.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            59997..60425
                     /locus_tag="SI621_000358"
     CDS             59997..60425
                     /locus_tag="SI621_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEI4063115.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(60464..60808)
                     /locus_tag="SI621_000359"
     CDS             complement(60464..60808)
                     /locus_tag="SI621_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEI4063116.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYETLFTYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(60885..61019)
                     /locus_tag="SI621_000360"
     CDS             complement(60885..61019)
                     /locus_tag="SI621_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063117.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            61095..63656
                     /gene="mutS"
                     /locus_tag="SI621_000361"
     CDS             61095..63656
                     /gene="mutS"
                     /locus_tag="SI621_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEI4063118.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            63762..>63862
                     /locus_tag="SI621_000362"
     CDS             63762..>63862
                     /locus_tag="SI621_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEI4063119.1"
                     /translation="MPSTRYQKINAHHYRYIWAVGDIHGDYQLLQSR"
BASE COUNT        15137 a        17226 c        16527 g        14972 t
ORIGIN      
        1 atcccattac gcttttatta aggagcatta gcatgttctc accgcagtca cgcttgcgtc
       61 atgcagttgc agatacgttc gcgatggttg tttactgttc tgtcgtgaac atgtgtattg
      121 aagttttcct ctccggaatg agcttcgaac aatcttttta ttccagattg gtagcgattc
      181 cggtgaacat cttaattgca tggccatacg gtatgtaccg cgatctgttt atgcgcgcag
      241 cacgcaaagt tagcccgtcg ggctggataa aaaatctggc ggatatcatg gcttatgtga
      301 cgttccagtc accggtgtat gtggcgatct tgttagtggt gggcgcagac tggcatcaga
      361 ttatggcggc ggtcagttca aacatcgttg tttcgatgtt gatgggggcg gtttatggct
      421 acttcctcga ttattgccgc cgactgttta aagtcagccg ttaccagcag gtaaaagcct
      481 gacccttctt ttcgcgactg gcatcgccag tcgcgtcttt aactggcttc gccaaataaa
      541 ccattcaaat aacgttcaag cgcaacacgc gaactaaagc catgcgggat accgtaatgc
      601 tcatgacgtt cacccgctaa atagagcgga aattgctggt aatgatcgca agttttaata
      661 tcggaagcgg gttcagccag tgttgaacta cgttctttta atgttggatg aatcaccagg
      721 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
      781 ccataagctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
      841 acctcgtctg gtcgtaaata catatttgat cctcgttatt atcaaccgcg ggcctacctt
      901 acctgattgc gcattcacaa cgctttcaga aaagtccata aagccgcgat gaacctcagt
      961 taagaaaatt atggtctaca ctgaaaatta catcgaattc taatggggga tcatatgttt
     1021 aaccgaccga accgcaacga cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat
     1081 caattagctg acagcctcga atctgtattg aagtcctggg gcagcgacgc caaaggggaa
     1141 gccgaagccg cacgcagtaa agcgcaggct ctactgaaag aaaccagggc gcgaatgcat
     1201 ggtcgtactc gcgtccagca agccgcgcgt gatgccgttg gctgcgcgga ttcttttgtt
     1261 cgtgaaagac cctggtgtag cgtgggtaca gcagctgcgg taggtatttt tatcggcgca
     1321 ctgttaagca tgcgcaaatc gtagtgcaat aatgataata aatacgcgtc tttgaccccg
     1381 aagcctggct tcggggtttt ttagcttaag gctttacgta attgttgtgc tacctgtcgc
     1441 gccatacctt cgttaacgat attagtaaac gagagcagta atccaccttc cccacttgaa
     1501 cggatacgcc agtcgctaag tgcctgcacc gcaaggcctg cagcattggc tttacgcgca
     1561 tgggcaatat catcgcctat cattctgatc accatctgga taccaccttt ctgcggcaca
     1621 acctgaaatc cttgctgcgt caacgcttgc tcaatccatt gccgacgttg ggcataatgc
     1681 tgacgcattt tcttcagatg ccgccagaaa tgcccctcgc gaagaaagtc tgccagtgtg
     1741 ttctgccata gaacaggtac cgcacatgga gccagtgacg cctggtggcg gaattgtgca
     1801 atttgcttca ctggcaacac cagccacgca cagcgcaatg caggaaatag cgctttgctg
     1861 aatgttccgg cataaattac ccgctgcggt gcgtcgagac tttttaacgc cggtaacggc
     1921 ttaccgtgat agcgaaactc actgtcataa tcatcttcaa taatccatgc ctgactacga
     1981 tctgcccatt ccagtatctg atgcctgcgc gctaaagaga gcgccacacc cagcggactt
     2041 tggtgtgctg gtgttatcag ggcaaaacgc gcatcaggat aattttgtat tccgcttgtg
     2101 atatccagtc cattgtcatc aaccggcaca ggcaaaattt ccacatcatg gcgagtgaca
     2161 atcgggcgaa tcagtggaaa gccgggatct tctatccaca tcccgtttcc cggtttcgcc
     2221 agcgcgtgca gaattaaagc tattgaggcc gcataaccgt gagtgataaa gacctgctct
     2281 ggctgacaat caatcccacg tgaaacgcgt aagtaatcaa ctattgcctc gcgcaacgcc
     2341 acctcaccgc agacatcgcc taacgccaaa tcaaagcgcg tctgggtacg aaggcgacgc
     2401 cccatcaccc gcgcccacag ctctcgcgga aagagatcga gtgcgggtaa tcccatttga
     2461 aatggcaacg gttgctgact ttcaccggca aaagctacaa cttgtacagt agattgttgc
     2521 ggatatatcc gctcactgac aaatgtgcca gcctgtcccc gcctgatcag ccatccttgc
     2581 gccaccagct cagcatacgc attttcgaca gtggatcgag acacccccag ctcctgcgac
     2641 caggtacgac ttgaaggcag ccttgcgccg ggtttcagtt ccccttgctc aatagccgtt
     2701 tttaactgac gggcgatatc ctgatagcgc ggcatatggt ctgctttttt aggtaattta
     2761 tggctctctt tagcagtcca ttatagtgct acattgcggg cgttaataag aggagaaact
     2821 gtatgactac attacgtcag ccttactacg aacttagccc ggcagtgtat aacgcactag
     2881 tgcaggccaa aacggcactg gaaaatagca cgctggatac cacgttgatg gagttagttt
     2941 atttgcgcgt ctcgcaaatc aacggctgcg cattttgtct ggagatgcac agcaaagcat
     3001 tgcgcaaatc cggcgtgcca cagcacaaac tggacgccct ggcaggttgg cgcgtaagcc
     3061 atcattttga tgaacgcgag cgggcggcgc tggcatgggc ggaatcggta accgaaattg
     3121 ccagaaccca tgcggaagac gaggtttatc agcctttgct tgagcatttc agcgcagcag
     3181 agatcagcga cttaacgttt gccatcgggc tgatgaattg ttttaaccgt ctggccgttt
     3241 ccatgcggat gtaacttctt gatgccgggt attcgcccgg caaaaaaaca caaaatcccc
     3301 taccccgttg agccattcat cggtggcgta atcaatctaa aattcccata tcttgtatgg
     3361 ttgaatcttt tttcaactac atctagtatc tctgtatcaa cagagagaca acccgacgcg
     3421 tatcatcgcg ccgtatcttc attttaaacg gaaatacgaa taatgcgcat tactatttac
     3481 actcgtaacg attgcgttca gtgccacgcc accaaacggg cgatggaaaa ccggggcttt
     3541 gactttgaaa tgattaatgt cgatcgcgtt cctgaagcgg cagaagcgct gcgtgctcag
     3601 ggctttcgtc agttgccggt tgtgattgca ggcgatctta gctggtctgg tttccgcccg
     3661 gacatgatca atcgtctgca tccagcgcca cacgctgcca gtgcatgagc cagctcgtct
     3721 acttttccag cagctccgaa aatacgcagc gttttatcga acgtttaagg ctgcccgcgg
     3781 tgcgcatccc gctcaatgag cgggaacgga ttcaggtaga cgagccttac atcctgatcg
     3841 tgccctctta cggcggcggc ggtacggctg gcgcggtgcc acgacaggtc attcgctttt
     3901 taaacgacga gcataaccgg gcgttgctgc gcggcgttat tgcttcgggt aatcgcaact
     3961 ttggtgaggc gtatggccgc gccggagatg tgattgctca gaaatgcggc gtgccgtggc
     4021 tgtaccgttt tgaactcatg ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa
     4081 ccgaattttg gcaacgacac ccgcagaacg cctgacgcag gaaacgatgg attaccacgc
     4141 gctgaatgcg atgcttaacc tttacgatag cgcaggtcgc attcagttcg ataaagatcg
     4201 ccaggcagta gacgccttta taacgacgca tgtgcgtccg aacagtgtgg ccttcagtag
     4261 ccagcagcag cgcctgaact ggctggttaa cgaaggttac tatgatgaaa gcgtccttaa
     4321 tcgctactct cgcgattttg tcattacgct gtttgcccac gcgcacgcca gcggttttcg
     4381 tttccagaca ttcctcggag catggaagtt ttacaccagc tatacgttga agacattcga
     4441 cggtaagcgt tatctggaag attttgccga tcgagtcacg atggtggcgc tgacgctggc
     4501 acaaggcgat gaaacactgg cgacgcaact gaccgatgag atgctgtcag gacgctttca
     4561 gccagccacg ccgacattcc tcaactgcgg taagcagcag cgcggcgaac tggtttcctg
     4621 ttttctgctg cgtattgaag acaatatgga gtcgattggc cgggcggtaa attccgcact
     4681 gcaactgtcg aaacgcggcg gcggcgtagc atttttgctg tcgaatctgc gagaagcggg
     4741 cgcgccaatt aaacgtattg aaaatcaatc atctggcgtg attccggtga tgaaaatgct
     4801 ggaagacgca ttttcctatg ccaaccagct cggcgcgcgt cagggggcag gtgcagtcta
     4861 tttacatgct catcatcccg atattctgcg ttttctcgat acaaaacggg aaaatgccga
     4921 cgaaaaaatc cgcattaaaa cactgtcgct gggcgtggtg atcccggata tcactttcca
     4981 tctggcaaaa gagaatgcgc agatggcgct gttttcgcct tatgacgtag agcgagttta
     5041 tggcaagccg tttgctgata tcgccatcag tgaacactat gccgaactgg ttgccaatga
     5101 acgcatccgc aaaaaataca tcaacacccg tgatttcttc cagcgactgg cagaaatcca
     5161 gtttgagtcc ggctatccct acatcatgta tgaagacacg gttaaccgtg ctaaccctat
     5221 cgccgggcgc ataaatatga gcaatctctg ctcagaaatt ttgcaggtta acagcgcctc
     5281 agagtatgac gagaatctcg actatgcccg cacaggccat gatatttcct gcaatttagg
     5341 ttcgttgaat attgcccaca ccatggattc ccccgacttt gcccacacgg tagagactgc
     5401 cgtgcgcggt ttaacggcag tatcagatat gagtcatatc cgcagcgtgc cgtccatcga
     5461 agccggaaat gccgcctcgc acgccatcgg actggggcag atgaatttac acggttatct
     5521 ggcgcgagaa ggcatcgctt atggatcgcc ggaagcactc gatttcacca atctctattt
     5581 ctataccatc acctggcacg cactgcgtac ctcgatgttg ctggcgcgcg aacgcagtga
     5641 aaccttcgct gggttcaaac agtcacgcta tgccaatggt gaatatttca gccaatatct
     5701 gcaagggaac tggcagccga aaacggcgaa agttggcgaa ctgtttgccc gtagcggtat
     5761 tacgttacct acccgtgaga tgtgggcgca actgcgcgac gacgtgatgc gctacggcat
     5821 ttacaaccag aatcttcagg cggtgccgcc aaccggttct atctcttata tcaaccatgc
     5881 tacgtcgagt attcatccga ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg
     5941 acgcgtttac taccctgccc cgtttatgac taacgagaat ctggcgctgt atcaggatgc
     6001 ttacgaaatc ggcgcagaaa agatcatcga cacctacgcg gaagcgactc gccatgtcga
     6061 tcagggtttg tcgctgacgc tgtttttccc cgataccgcc accactcgcg atatcaacaa
     6121 agcgcagatt tacgcctggc gcaagggtat caaaacgctc tattacattc gcctgcgtca
     6181 gatggcgctg gaaggcactg aaattgaagg ctgcgtctcc tgtgcacttt aaggaatatc
     6241 tatgaaactc tcacgtatca gcgctatcaa ctggaacaag atatctgacg ataaagatct
     6301 ggaggtgtgg aatcgcctga ccagcaattt ctggctacca gaaaaggtgc ctctgtcgaa
     6361 cgatattcct acctggcaga cattaactgt cgtagaacaa caactgacga tgcgcgtttt
     6421 tactggcctg acgctgctcg acacgctgca aaatgttatc ggcgcgcctt cgctgatgcc
     6481 cgatgcgctc acgcctcatg aagaggcggt attatcgaat atcagcttta tggaagcggt
     6541 tcatgcccgc tcttacagtt cgattttctc gacgctgtgc cagaccaaag atgtcgatgc
     6601 cgcctacgcc tggagtgaag aaaacgcgcc gttgcagcga aaagcacaga ttattcagca
     6661 acattatcgc ggggatgatc cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt
     6721 tttgttctat tccggtttct ggctgccgat gtatttttcc agccgcggaa agctaaccaa
     6781 taccgcagac ctgatccgtc tgattatccg cgatgaagca gtccacggtt attacatcgg
     6841 ctataaatat cagaaaaaca tggaaaagat ctcccaggca caacgtgaag agttgaagag
     6901 tttcgccttc gatttgttgc tggaacttta tgacaacgag ttgcaataca ccgatgagct
     6961 gtacgccgaa accccgtggg ctgacgatgt gaaagcgttt ctctgttaca acgccaataa
     7021 ggcgttgatg aatctgggct acgaaccgtt atttcccgca gaaatggcgg aagtgaatcc
     7081 ggcaatcctc gccgcgcttt cgccgaatgc cgatgaaaat cacgatttct tttccggttc
     7141 aggctcttca tatgtgatgg gaaaagcggt cgaaacagaa gatgaagact ggaatttctg
     7201 agggtgttat ttttcaaaaa tatcactacc cgcagcaggg aaataatttc cgccaaatag
     7261 ctttttatca cgcaaatatt ttgtggtgat ctacactgac actctgttgc attattcgcc
     7321 tgaaaccaca atattcaggc gttttttcgc tatctttgac aaaaaatatc aacttttctc
     7381 gattcactct cagcccttat atcacgggaa attccggcga tttgctctca tcaatattca
     7441 tgccacattt gccgtcaggg gttgcctcag attctcagta tgttagggta gaaaaaagtg
     7501 actatttcca ttgggtaatg tatcgacata aacaaataac aggaatcttt ctattgcatg
     7561 gcaattaaat tagaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagct
     7621 ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac tgggctatcg
     7681 cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga
     7741 ttatccggtt cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc
     7801 cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt
     7861 gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg
     7921 accgtgctgg acaatactgc gttcggtatg gaactggccg gaattaatgc cgaagaacgc
     7981 cgtgaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac
     8041 ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt
     8101 aatccggata tattattaat ggacgaagcc ttctcagcac tcgatccatt aattcgcacc
     8161 gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt
     8221 tcccacgatc tcgatgaagc tatgcgtatt ggcgaccgaa ttgccattat gcaaaatggt
     8281 gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc
     8341 cgtaccttct tccgtggcgt cgatattagc caggtgttca gtgcgaaaga tattgcccgt
     8401 cgtacaccga acggcttaat tcgtaaaacc cccggcttcg gcccgcgttc ggcactgaaa
     8461 ttattgcagg atgaagatcg cgaatatggc tacgtcatcg aacgcggtaa taagtttgtc
     8521 ggcgctgtgt ctatcgattc gcttaaagcc gcgttaacgc agcagcaagg tcttgatgcg
     8581 gcgctgattg atgcgccgtt agcggtcgat gcacaaacgc ctcttagcga gttgctctct
     8641 catgtcggac aggccccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc
     8701 atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga
     8761 tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcacagt ccgcagacgc
     8821 ctggggtaca ccggcgactg caccgactga cggcggtggt gctgactggc tgaccagtac
     8881 gccagcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc
     8941 gctcgacagt tgggtcactg aagggatcga ctgggtcgtt acccatttcc gtcccgtctt
     9001 ccagggcgtg cgcgttccgg ttgattacat cctcaacggt ttccagcaat tgctgctggg
     9061 tatgcccgct ccggtggcga ttatcgtttt cgctctcatc gcctggcaaa tttccggtgt
     9121 cggaatgggc gtggcgacgc tggtttcgct gattgccatc ggcgcaatcg gtgcctggtc
     9181 acaggcaatg gttactctgg cgctggtgct aaccgccctg ctgttctgta tcgtcatcgg
     9241 tttgccgttg gggatctggc tggcgagaag tccacgagcg gcgaaaatta ttcgtccact
     9301 gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtaccaatcg tcatgctgtt
     9361 tggtatcggt aacgtgccgg gcgtagtggt gacgatcatc tttgctctgc cgccgattat
     9421 ccgtctgaca attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc
     9481 attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac
     9541 cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg tcatcgcctc
     9601 gatgattgca gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat
     9661 ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgaccgcct
     9721 gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg
     9781 ccctgttggt ttgctgaccc gcccattcat taagtaactc tgcacttgcc cggtgacgcc
     9841 gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac
     9901 agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat
     9961 tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc tgctggtcag
    10021 tcgtgcactg gagaaattag gttataccgt caataaaccc agcgaagtgg attacaacgt
    10081 tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccgct
    10141 gcatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggagtatt
    10201 tgttaacggc gcggcacagg gttacctgat cgataagaaa actgccgacc agtacaaaat
    10261 caccaacatc gcgcaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga
    10321 cggtaaagcg gatttgactg gctgtaaccc tggctggggc tgcgaaggtg cgatcaacca
    10381 ccagcttgcc gcgtatggac tgaccaatac cgtgacgcat aatcagggga actacgcagc
    10441 gatgatggct gacaccatca gtcgttacaa agagggtaag ccggtgtttt actacacctg
    10501 gacgccgtac tgggtgagta acgaactgaa accggggaaa gatgtggtct ggttgcaggt
    10561 gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa
    10621 ttacggcttc ccggtcagta ccatgcatat cgttgccaac aaagcctggg ccgagaaaaa
    10681 cccggcagca gcgaaactgt ttgccattat gcagttacca gtggcagata ttaacgccca
    10741 gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc acgttgatgg
    10801 ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca
    10861 gaagtaaata ttgataaccg cgcagataat gctcatgtga gtaaaacaat tgcagcgatg
    10921 gttttaatcc ggctgaaaat cgatgagacg ttttctgaag gccggggcga cgcgcaggga
    10981 tgcgcggcga gggcgcgtct tacagggatg ttacctcgcg cccgtacccg atagccgtaa
    11041 gaaataagtc gaatgtaccg cgtagcggcg attttgtgcc aaaagtttgg gtcgccaggg
    11101 tggtggcgac tgagccaccc tggcacgttc accagcaaaa tagtgacaaa gtagctataa
    11161 tctggaagtg aacgtaacat ttgccttagt ggcatatttc accttcaaat accctctccc
    11221 cattacccgc acattttaac aatctatctt tcgccccata ttcatcaaca tccgctatta
    11281 ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc taatcatgag
    11341 cttagcccgg cactgatcgt actgatgtct atcgccaccg gtctggcggt cgccagcaac
    11401 tattacgccc agccattgct cgacaccatt gcgcgtaact tttccctttc cgccagttcg
    11461 gcgggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctgct gtttcttgtt
    11521 cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcagcg
    11581 ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatcatcct cggtacggca
    11641 ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct
    11701 tcaccggata aacgcggcaa agtggtcggc accattatga gcgggctgct gttggggatc
    11761 ttgctggcac gaaccgttgc cgggttgctg gcaaacctcg gcggctggcg caccgtcttt
    11821 tgggttgcct cggtgttaat ggcactgatg gcgctggcat tatggcgtgg tctgccacaa
    11881 atgaaatcag aaacccacct caactaccca cagttgcttg gttccgtttt cagcatgttt
    11941 atcagcgata aaattctgcg cacccgcgcg ttgctgggct gcctgacctt tgccaacttc
    12001 agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat
    12061 ggtgtaattg gtctgtttgg acttgcggga gctgccggag cgttgggtgc tcgtccggcg
    12121 ggcggttttg ccgataaggg taaatcgcac cacaccacta ctttcggtct gctgctgcta
    12181 ttactttcat ggctggcgat ctggtttggc cacacttccg tactggcctt gattatcggc
    12241 atcctggtgc tggatctcac cgtgcagggc gtgcatatca ctaaccagac ggtaatttat
    12301 cgaatacacc ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt
    12361 ggcggtgccg ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc
    12421 gtttgtctgg ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt
    12481 catcgtcagg aagctgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata
    12541 agggtgttaa gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa
    12601 tattaacaat cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt
    12661 tcggcgacct tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg
    12721 gtggcctttg cgttcggtct gaatgcgacc cgtctgggat tctctcctct caaaagcgtt
    12781 tttttctcct gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca
    12841 gccgggagta gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt
    12901 ttgtatggcc cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc
    12961 ctgtgggcat ttggcctgac ggatgaggtt tttgccgccg caaccgcaaa actggtacgc
    13021 aataatcgcc gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg
    13081 tgggtatttg gtacggtaat aggggcattc tccggcagcg gcttgctgca aggatatccc
    13141 gccgttgaag cagcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc
    13201 tctttccagc gcaaacaatc tctttgcgtt accgcagcgt tagttggtgc ccttgcaggc
    13261 gtaacgctat tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact
    13321 gcgttaatcc aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg
    13381 gttactggtt ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg
    13441 taatgcccgc ccaaccaaac gtggcgcgat aggcatattg ctcgacacca ttggcatcgc
    13501 ctcgatatgc gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg
    13561 tttcgtgccc acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag
    13621 cattatcatc ccaacactgc ttagtgcgct ggcctacggg ctcgcctgga aagtgatggc
    13681 gattatataa cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg
    13741 tcgttactat atcggctgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc
    13801 cattgaacaa atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat
    13861 ccttctgacc cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat
    13921 gctgaaggct caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca
    13981 ggaaaaccac agtattcagc cttctgaatt gagttgtgct cttggatcat cccgtaccaa
    14041 cgcgacgcgt attgccgatg aactggaaaa acgcggttgg attgaacgtc gtgaaagcga
    14101 taacgatcgc cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga
    14161 ggttttacca ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga
    14221 aaaagatcag ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca
    14281 agacggtgtg gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt
    14341 ataaaagaaa aaatgactgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg
    14401 gctcagccga tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca
    14461 aaccccgcag caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac
    14521 cttgctcttt ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca
    14581 tttcgaagaa accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt
    14641 gtctggcagc gtgacgaaag tctgggccga taacaccgac tttgtaaaag aaggcgatgt
    14701 gctggtcacc ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact
    14761 ggcttccagc gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa
    14821 tattgaggtg caaaaaatcg cactcgcgaa agcacaaagc gactacaacc gccgtgtgcc
    14881 gctgggcaat gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgttac
    14941 cagtgcccag gcacaactgg acgtcgcgat tcaacaatac aatgccaatc aggcgatgat
    15001 tctggggact aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa
    15061 cgcctggctg gcgctggagc gtactcgtat tgtcagtccg atgaccggtt atgtctcccg
    15121 ccgcgcggta cagcctgggg cacaaattag cccaacgacg ccgctgatgg cggtcgttcc
    15181 agccaccaat atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat
    15241 cggtcagccg gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa
    15301 agtggttggt ctggatatgg ggacaggtag cgcgttctca ctgcttccgg cgcaaaatgc
    15361 gactggtaac tggatcaaag tcgttcagcg tctgcctgtt cgtattgaac tggaccagaa
    15421 acagctcgag caatacccgc tgcgtatcgg tttgtccacg ctggtgagcg tcaataccac
    15481 taaccgtgac ggtcaggtac tggcaaataa agtacgttcc acaccggtag cggtaagcac
    15541 cgcgcgtgaa atcagcctgg cacctgtcaa taaactgatc gacgatatcg taaaagctaa
    15601 cgctggctaa tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac
    15661 tggtcattat gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca
    15721 ttgctaacgt ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccagggaa
    15781 cgtgggtaat cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc
    15841 tggcaaagcg cgtcggggaa gtgaaactgt tcctctggtc caccatcgcc tttgctattg
    15901 cgtcgtgggc gtgtggtgtc tccagcagcc tgaatatgct gattttcttc cgcgtgattc
    15961 aggggattgt cgccgggccg ttaattccgc tttcgcaaag tctattgctg aataactacc
    16021 cgccagccaa acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gttgcgccaa
    16081 tttgcggccc gatcctcggc gggtatatca gtgacaatta ccactggggc tggatattct
    16141 tcatcaacgt accgattggc gtggcggtgg tgttgatgac actgcaaacc ctgcgcggac
    16201 gtgaaacccg caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg
    16261 gtatcggtag cctgcagatt atgctcgacc gcggtaaaga gctggactgg ttttcatcac
    16321 aggaaattat catccttacc gtggtggcag tggtggctat ctgcttcctg attgtctggg
    16381 agctgaccga cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca
    16441 tcggctgttt gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc
    16501 cgcagttgtt gcaggaggtc tacggttaca cggcaacgtg ggcaggtctg gcctctgcgc
    16561 cggtaggaat tattccggtg atcctgtcgc cgattatcgg ccgcttcgcg cataaactgg
    16621 atatgcggcg gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg
    16681 cctatacctt tgaaccgggt atggattttg gcgcgtcggc ctggccgcag tttatccagg
    16741 ggtttgcggt ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac
    16801 cggaacgact ggcggcggca tcgagcctct ccaactttac gcgtacgctg gcggggtcta
    16861 tcggcacgtc gataaccaca accatgtgga ccaaccgcga gtcgatgcac catgcgcagt
    16921 taaccgagtc ggtaaacccg tttaacccaa acgcccaggc gatgtacagt caactggaag
    16981 gactgggaat gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg
    17041 ggctgattat ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtcttgc
    17101 tggggctggt gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggtggtgcgc
    17161 actaagtaca actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag
    17221 tttactgact agatgtgcag ttcctgcaac ttctctttcg gcagcgccag ttcttcgttg
    17281 ctgttgatgc gcacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac
    17341 gagtgcatct ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc
    17401 tgcactttca gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct
    17461 ggcgtaccaa tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata
    17521 atctctacac cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg
    17581 gtatggatcc ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac
    17641 acggtgattg cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct
    17701 tccatccggg tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta
    17761 atttttttaa aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg
    17821 cgcatttgtt atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc
    17881 tttttctttt ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg
    17941 cttccatttc ctgctgacgg cgttcagacg cctcgcgctc ggctacaaaa tcctcttcgc
    18001 gcagaatttc caacggctct tcacgcagca gattacggta cgcttcagca aacgctttgc
    18061 ctgttccgcc aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat
    18121 cgggattatc gaagcaggcg accagttcat cacacacttt ctgatacgct tcgccgccgt
    18181 taatgctatc cagcgtttgc gcaacgcgtt tcagatcgcg gaacagatct ttacccacct
    18241 gcggtaacgg gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac
    18301 cttcgaggat cacccggttc cagttaacgc gtgtacaggc aagttcttta ctgctcattt
    18361 ccggtgcatc agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt
    18421 catctacacc aatcggcgag aatgggttga tgtccagcga acgcacttca atatattcaa
    18481 tgccgccacg taacagcgca tcagaaggcg actcgccgct gcgggtaacg cgttttggac
    18541 gaatcggcgc gtacagttcg ttttcaatct gcagcacgtt gctgttgatt tgcagcctct
    18601 taccatcttt ctcaatgcca atcttggcgt actcttccga tggcgttttg attgcctgtt
    18661 taaggcccgc tacgtactcg taaagatcgt tgaaggtaat acctagattg ctttgcgatt
    18721 tattggtata gccgagatca ctcaaacgaa gagaggtcgc atacggcagg taatacatac
    18781 cgcactcggt tttctcaaac ggcagcgatg ttggttttcc ttgcaggaaa gaagaacaaa
    18841 tcgccggaga cgcgccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga
    18901 taacgcggaa atagcccgca gaaattttct ctttggcatc agcgcccgcg atatcaccac
    18961 acttcgcttg ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt
    19021 gcatcagcgc gccgtagcga tttttcagcc cttcacggta cagcgtttta aagcgtccgg
    19081 tgttagaagt gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca
    19141 tacttaacgg ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc
    19201 gcataaaggt cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg
    19261 cttccgcaaa atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat
    19321 gacctgttgt tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac
    19381 gctgtatccc ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt
    19441 ccgggatcaa attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt
    19501 gaccatatgt gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg
    19561 caactgcaac atagcgtaac gctttaccaa ggcacaaaaa aaagattacc ggcccccagg
    19621 agatgcgcat ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta
    19681 ataatagcgt gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg
    19741 atgttttacg caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat
    19801 tacccattgt tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg
    19861 ccaccagcac gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa
    19921 acaacgagaa aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc
    19981 tgctgcacgg gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgtgtcgg
    20041 ttgcacgccc atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt
    20101 gacgtgatca gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa
    20161 tgcctcagcg atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca
    20221 gcttttcacc acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac
    20281 tgcttctgtt ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat
    20341 aatcgcctga gcaatacgcc aggtgggcga gccattaagc gcaatcatcg cctgaacatc
    20401 gtactgaaga ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctcggt
    20461 atccaggatg gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt
    20521 ctcgcaagtc gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg
    20581 cgaaggagaa gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg
    20641 aaggaaattt tttggagaag atggtgcatc cgggaggatt cgaacctccg accgctcggt
    20701 tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaaact tattttactg
    20761 ctgatattga taccgctact aaagccatat caaataagaa aatccatctt aaccatcatc
    20821 actgatgatt acggagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt
    20881 cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaattta ttttactgct
    20941 gatattgata ccgctgctaa agccatatca agtaagagat ggtgcatccg ggaggattcg
    21001 aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg
    21061 ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc aagtaagaga
    21121 tggtgcatcc gtgaggatta ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa
    21181 cgttatcctc cctggtgcct gcggttatta ccgccacctc ttccatacct tcatcgctga
    21241 aagtacgtag aatatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
    21301 cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc
    21361 cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca
    21421 cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc
    21481 gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca cacttttatc
    21541 gtttgcacac ttcacgctca attagtctgt aaaaacgtca aaaagagtgt tttatcaaca
    21601 gaaaaacgga ggtctgtcag atagcagtaa tgcaaaaaaa tggagactta agttgaatga
    21661 acgggagtaa agcgaaaaga caatggagtg aaggagatat tttgaggggt gcgtctcacc
    21721 gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc agcctggata
    21781 cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac gccaatacgt
    21841 acctgattgc cctttacccc taaaactgtc acggtgacct catccccaat catgagggtc
    21901 tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta aaagagtcgg
    21961 gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca atgacaaaca
    22021 cattacatgt aagcagtcat ggcattacat tctgttaaac ctaagtttag ccgatataca
    22081 caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc
    22141 cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc tttcacactg
    22201 gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt
    22261 ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc tgctttcaca
    22321 cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc ggctaccgtt
    22381 gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat ggtacgcaac
    22441 attttcggct caacaccgct aagctcgcta accaacagct taacaccatt aacatcaatt
    22501 gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag ctgttgtaac
    22561 tctttttcca gctgacgcgt acgttccagt actgagcgca ctttatcagc cagattattg
    22621 ctatcgcctt tcagcagatg cgcgacttcg tttaagcgat cgctgtctgc atgaacggtg
    22681 gcgatagcgc cttctccggt taccgcttcg atacgacgaa cgcctgcagc agtacccgat
    22741 tcagagatga tgcggaacag accaatatca ccagtgcggc tggcgtgagt accgccacac
    22801 agctcggtgg agaaatcgcc catgctcagc acgcgtacgc gctcatcata cttctcgccg
    22861 aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc
    22921 ggcaagttac ggcgaatctg tgcgttcacc aggtcttcga ccgcacgaat ctcttctggt
    22981 ttcatcgctt cgttgtgtga gaagtcgaag cgcagcactt tgtcgttaac cagtgaacct
    23041 ttctgcgata catgagtacc cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg
    23101 gagtgattca gacgaatacg ggcgcgacga gcctcatcaa catcagcctg caccgcgtcg
    23161 cccactttca gagaacccgc agcaagttta ccgatgtgac caatcgcctg gccgtatttc
    23221 tgcgtatctt ccaccgcaaa ggagaagtta gcgcctttca gttcgccttt atcaccaacc
    23281 tgaccgccgg attccgcata gaatggcgtt tgatccagca cgaccacagc ttcctggcct
    23341 gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt gccgttcagt
    23401 tccagatggt catagccttt aaattcagat gcgctgtcaa cacggatcat tgcgttgtaa
    23461 tcggcaccaa agccgctggc ttcgcgcgcg cgacgacgct gctcttccat tgctgcttcg
    23521 aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt caggtcaacc
    23581 gggaagccat aggtgtcgta cagacggaaa gccgtttcgc catccagcgt atcaccagaa
    23641 agttttgcca gctcttcatc cagcagcgcc agaccgcgct ccagagtacg ggcaaactgc
    23701 tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc
    23761 gcagagccca ttacgtcgat cagcggacca accagtttgt agaagaaggt ttctttcgcg
    23821 cccagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac ataaccacgg
    23881 ttttcattgg acggcattac gccatccgcg atcaggaacg cacaagaacg aatgtggtca
    23941 gcgattacgc gcagcgattt attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc
    24001 tggatcagcg tgcggaacag gtcgatgtca tagttagagt taacgtgttg cagcaccgca
    24061 gcaatacgct ccagacccat accggtatct acagacggct tcggcagcgg ttccatcgtg
    24121 ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat gtagcgatcg
    24181 ccgtcttctt ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag
    24241 atttcggtgc acgggccgca cggaccagtg tcacccatct gccagaagtt gtcagatgcg
    24301 tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat ccctacttct
    24361 ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca cagacgctct
    24421 ttcggcaggg caaaccattt ttcactggtc agcagttccc atgcaaactg aatggcatcg
    24481 tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt atgatgacgc
    24541 gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg
    24601 gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc cttgaactgg
    24661 ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat ggggtaccag ggagctgctg
    24721 gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg gatctcagcg
    24781 gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgccac cagctccagc
    24841 gtgtcttaac cgccgggctg gtaactgaaa agtgggaata agataagttt tcttgactgg
    24901 gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc caaatatcct ggatatcttc
    24961 catcagatag ccacgataga gcagaaaacg ctggatctta actttttctg aaaagacagt
    25021 tggcaaaggt tcgccatatt ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat
    25081 gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct gattcagttc
    25141 ctgacgaata cgcgcaggtc cataaccttt gcggctacgg ctggcgataa agcgcgcaac
    25201 aaatcggctg tcatcgagat agccatgttc atggcaccag gcgataacgc gctcgtaatc
    25261 ttctgccgtg gcatcaatct cttccgggcc atttttgccc ataatcggtg ccgcgagttt
    25321 acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa
    25381 caggcgagca tatgccgggc gacgggacgt tgattctgtc atggcatatc cttacaactt
    25441 aaaaaagcaa aagggccgca gatgcgaccc ttgtgtatca aacaagacga ttaaaaatct
    25501 tcgttagttt ctgctacgcc ttcgctgtca tctacagaga aatccggcgt tgagttcggg
    25561 ttgctcagca gcaactcacg tactttcttc tcgatctctt tcgcggtttc cgggttatct
    25621 ttcagccagg cagtcgcatt cgctttaccc tgaccgatct tctcaccttt gtagctgtac
    25681 cacgcacctg ctttctcgat cagcttctct ttcacgccca gatcaaccag ttcgccatag
    25741 aagttgatac cttcgccgta gaggatctgg aattcagcct gtttaaacgg cgcagcgatt
    25801 ttgttcttca ccactttcac acgggtttcg ctacccacca cgttttcgcc ctctttcacc
    25861 gcgccgatac gacggatgtc gagacgaaca gaggcgtaga atttcagcgc gttaccaccg
    25921 gtagtggttt ccgggttacc gaacatcaca ccaattttca tacggatctg gttgatgaag
    25981 atcagcagcg tgttggactg cttcaggtta cccgccagct tacgcatcgc ctggctcatc
    26041 atacgtgccg caaggcccat gtgagagtcg ccgatttcgc cttcgatttc cgctttcggc
    26101 gtcagtgccg ccacggagtc aacgacgata acgtctactg caccagaacg cgccagggcg
    26161 tcacagattt ccagtgcctg ctcgccagtg tccggctggg agcacagcag gttgtcgata
    26221 tcgacgccca gtttacgtgc atagattggg tccagcgcgt gttcagcatc gataaacgca
    26281 caggttttac cttcacgctg cgctgcggcg atcacctgca acgtcagcgt ggttttaccg
    26341 gaagattccg gtccgtagat ttcgacgata cggcccatcg gcagaccacc tgccccaagc
    26401 gcgatatcca gtgaaagcga accggtagag atggtttcca catccatgga acggtcttca
    26461 cccaggcgca tgatggagcc tttaccaaat tgtttctcaa tctggcccag tgctgccgcc
    26521 aacgctttct gtttgttttc gtcgatagcc atttttactc ctgtcatgcc gggtaatacc
    26581 ggatagtcaa tatgttctgt tgaagcaatt atactgtatg ctcatacagt atcaagtgtt
    26641 ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca gtagcctgac gacgcaccgc
    26701 atcacggtca ccgctgaagc attcccgccg ggtaatacct tcgccgcggg cggtggcaaa
    26761 agcaaaccag acggtgccga caggcttctc ttcactgccg ccatccggcc cggcgatacc
    26821 actaatagac acagcataat cagcacgagc cgctttcagt gcgcctatcg ccatttccac
    26881 cacgacaggt tcgctcaccg cgccatgctg cgccagcgtc tcttcgcgta caccgatcat
    26941 ttgcgctttc gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg cggaactgcc
    27001 ggcaatatcg gtaatcactt tcgctaccca accaccggta caagactcag cagttgttac
    27061 ggttgcgcca cgggctttca gcgcctgccc aacctgttcg cttaactgca tcagttcact
    27121 gtcagtcatc acaacctctg tcagtcaaaa atttatgccg gacaagatag cactttcgtc
    27181 aggaagatgg tgttggcgtt gcaattttac gaggaggatt cagaaaaaag ctgattagcc
    27241 agagggaagc tcacgctccc ctcttgcaaa tagttactgc acgcgcgcca gcgccacggc
    27301 ttgtcctaac tgccagaccg ccattgcgta atgggtactg tggttgtaac gggtgatggt
    27361 gtagaagttc ggcagaccgt accagtactg gtaaccggta ccgacatcca gacgcagcag
    27421 gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt aaacctgccg cggcaagctg
    27481 cgaaatgctg tacttggttt tgaagccatt tggcaagcct ggagcctgac cgtttgccat
    27541 caccgcaacc tgatcacctt tcacccagcc gtgcgctttg aaatagttcg ccacactacc
    27601 aatggcatca accggatccc acaggttgat atgcccgtcg ccgctgaaat ctaccgcgta
    27661 ttgtttgtaa gacgacggca taaactgtcc gtagcccatc gccccggcaa aggaaccttt
    27721 cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc aggaaagttt ccagctcgcc
    27781 agagaaatac tccgcgcggc gtgggtagtt aaatgacagc gttgccagtg catcgaggat
    27841 gcgagttttc cccatcacgc gcccccagcg cgtttcaacg ccgataatcc cgacgataat
    27901 ttccggcggt acgccataca cctgccacgc gcgattcagc gcatcttcat actgattcca
    27961 gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat tttttgcgat aacgcagcca
    28021 tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt tatccatcag
    28081 ccgcagtacc gaatccagac gtttcgcctg ggagagaatt tcctgcaact gctgacgatc
    28141 gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg gcattcgggt tattagcgaa
    28201 atcgccgccc atctgcatca cattgtgctg cggctcaagc aggaagccgc cagacggcgt
    28261 tccggtggtc gtttcagtct cagtaggttt tggcttgctg ctacaggcgg caagcaacac
    28321 aaaaagggga agcaatgtta cataacgacg cttgaacatg aggggtccat ttaacagatt
    28381 caaccagggg caagtatggt aaagcatcac gccccgcaca aggaagcggt agtcactgcc
    28441 cgatacggac tttacataac tcaactcatt ctcctcgcta tccttttatt taaactttca
    28501 agttaaaata tttatctttc attttgcgat caaaataaca cttttaaatc tttcaatctg
    28561 attagattag gttgccgttt ggtaataaaa caataaatcc tgaaggagag aacaatgata
    28621 gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg cggagaggtg
    28681 tttaccggga tggtgaccgg cattcttccg ctgttgatta gcctgctggt tatcatgaac
    28741 gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg ttgcgccggt
    28801 aaccctgttt cccgttacct gctgctaccg tgcattggca cgtttgtctt ttgcaatccg
    28861 atgaccttaa gcctgggtcg ctttatgccg gaaaaataca aacccagcta ctacgcggcc
    28921 gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc aggcgaactg
    28981 tttgtttatc ttggcattgc cagcggtctg acaacactga acctgccact tggcccactg
    29041 gcggtgagtt atctgctggt tggtctggtc accaacttct tccgcggctg ggtgaccgat
    29101 ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg
    29161 gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc
    29221 cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc
    29281 ctgcgattgt tgacaaactg gcacagctta ctggctggca ggctattgac ggatttaaag
    29341 aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc ggcacattac
    29401 gctgcggcat ttatccgaaa cggcgtattc ccaccattaa tatccattcg acgggcaagt
    29461 ccggtccgct ggcgcagtat attgtggaag atatttatgt ctctggcgtg aaagaagaaa
    29521 acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt gactatgaca
    29581 ccagcaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga atgggtatgg
    29641 gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac catcgacact gtattaaaaa
    29701 ccattctgcc gtttatggcg ttcgtctcgg cgcttatcgg catcattatg gcttccggtc
    29761 ttggcgactg gattgcccac ggtcttgctc cgctggcgag ccatccactg gggctggtca
    29821 tgctggcgct catctgctcc ttcccgctgc tttcaccttt cctcggccca ggcgcagtta
    29881 tcgcacaggt tatcggcgta ttgattggcg tgcagattgg cctcggcaat attccgccgc
    29941 atctggcttt acctgcactg tttgccatca acgcgcaggc ggcctgcgac ttcatcccgg
    30001 tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc ccttctgttc
    30061 tggtgagccg ctttttaacc ggcgcgccaa ctgtactgat cgcctggttt gtctccggtt
    30121 ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat cacccgtatc
    30181 ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg tgaaggcgcg
    30241 cctgcggatc tcgaagaata ttgcttcatt cattgccacg gcgagttgaa aggtgcactc
    30301 catcccggtt tgcaattttc gctcgggcag catcgctatc cggtgaccgc cgttggcagc
    30361 gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga tggtttaaac
    30421 gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tccccgacga tatcgcgccg
    30481 ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc
    30541 atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat
    30601 cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca agaaattaac
    30661 gccgaatatg gtgaaggaac agcgtatggt tttggtgctg acgccaccag cgagcaaagc
    30721 gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt gctggtctac
    30781 agcgccggaa ttgcaaaagc agcctttatc agcgacttcc agctcggcga ttttgaccgt
    30841 tcgctacaag tgaatctggt gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg
    30901 atccgcgacg ggattcaggg gcgcattatt cagatcaact cgaaatccgg caaagtgggc
    30961 agcaaacaca actctggtta tagcgccgcg aaatttggtg gcgtcgggct gactcaatca
    31021 ctggcgctgg atctggcgga gtacggcatt acggtgcatt cactgatgct cggtaatctg
    31081 ctgaaatcgc cgatgtttca gtcgctattg ccacaatatg cgaccaagct gggtatcaaa
    31141 ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat
    31201 caggatgtgc tgaatatgct tctgttctac gccagtccta aggcgtcgta ctgcaccgga
    31261 cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga gatccgttaa
    31321 ggatctccgc gagactaata aaatgcctga tgcgctacgc tcatcaggcc tacaggactt
    31381 ccgccactac attaaggaaa agttatggta tccgcactca tcaccgtcgc cgttatcgcc
    31441 tggtgtgcgc aactggcctt aggcggttgg caaatttctc gttttaaccg tgctttcgac
    31501 acactatgcc agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt taaaccgcgg
    31561 gtcgtggtcg ccatcgcgct agacgatcag caacgcatcg tcgacacctt gtttatgaaa
    31621 ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa ttaccggtat gcatgcgggt
    31681 gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc tctatcattg
    31741 gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc gaagttatca
    31801 ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca ggccgccatt
    31861 ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca atactttgac
    31921 accacaggca caaccatccg caaagatctg gtcattctgg aacatgccgg aaccgtcatt
    31981 cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat cgatcataaa
    32041 actctcatca acacccacaa gaaagagctg attgcagaag ctgccgttag ttttatccat
    32101 gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt tcccctgctc
    32161 tcgcgcttta ataacatcac agtgatgacc aacagcctgc atatcgtcaa tgcgctatcc
    32221 gaactggata acgaacaaac tatcctgatg ccaggcggaa catttcgtaa aaaatcggcc
    32281 tcatttcacg ggcaactggc agagaatgcc ttcgagcatt tcaccttcga taaattgttt
    32341 atgggcaccg acggcatcga tctcaatgcg ggcgtaacca cgttcaacga ggtttatacc
    32401 gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcaga ctcatcaaag
    32461 tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa gctaattacc
    32521 gacgcaggta tcgatccggc gtttcgtcag gcgctggaaa agaaagggat cgatgtgatc
    32581 ataaccggag agagcaatga gtgaagcact actgaacgcg ggacgtcaga cgttaatgct
    32641 ggagttacag gaagcaagcc gtttaccgga acgtctgggc gatgattttg ttcgcgccgc
    32701 caatatcatc ctgcactgcg aaggtaaagt ggtggtttcg ggaattggca aatcgggcca
    32761 cattggtaag aaaatcgccg caacgcttgc cagcaccggc actcctgcct tttttgtcca
    32821 tccggcagag gcattgcacg gcgacctggg gatgatcgaa agccgcgatg tgatgctgtt
    32881 tatctcttac tccggtggcg cgaaggaact ggatctgatt attccgcgtc tggaagataa
    32941 atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc tggcggcgaa
    33001 agcggtgctg gatatttccg tagaacgcga agcctgcccg atgcaccttg cgccgacttc
    33061 cagcactgta aataccctga tgatgggcga cgcgctggcg atggcagtca tgcaggcgcg
    33121 cggttttaat gaagaagatt ttgcccgttc acatccagcc ggtgcgctgg gcgctcgctt
    33181 gctgaacaaa gtgcatcatc ttatgcgtcg tgacgatgcc atcccgcagg tggcgttaac
    33241 cgccagcgtg atggacgcga tgctggaact tagccgcact ggtctggggc tggtggcggt
    33301 atgtgacgct caacaacagg tacaaggcgt ctttaccgac ggtgatttac gtcgctggct
    33361 ggttggcggc ggcgcactca ccacgccagt taatgaagcg atgacgacag gcggcaccac
    33421 attacaggcg caaagccgtg ccatcgacgc caaagagatc ctgatgaagc gcaaaatcac
    33481 tgccgcaccg gtggtggatg aaaacggcaa actcaccggc gcaattaacc tgcaggattt
    33541 ctatcaggcc gggattattt aatccttcag ccccagacgt ttcgccagcc gatgcaggtt
    33601 ggcgacgtcg gtttccagca tccgcgcaca ggcggcccag ttgtgatgat tttgcgccag
    33661 cgcctgacga atggtttcac gctggaacgc ttctgtcgct tcacgcaggt tttgcttaac
    33721 aacgggcacc gccgccactt ctggcggcgg caacgtcacc tcaggaaaag caaaatgttg
    33781 cgcctcaaga atcacttcat cgccgctgcg ggttgctctc gccagcacta ccgcccgatg
    33841 aatagcatgt tccagttcac gaacgttccc cggaaaattg tagtgttgca gtaaatttcg
    33901 cgctccggca cttaatacca cgcgggagag ccccagccgc aaacgacact gctcgcagaa
    33961 ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca ctgaaagtgg
    34021 aaacacgctc aggcggtgga acaagtcagc gcgaaatcgc cctgccagta cctcttcgcg
    34081 taagtcacgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactgc ggtcatcgcc
    34141 aacgcgctga atatcgccat actgcaacac ccgcagcagc ttggcctgca atgccaacga
    34201 caactcgccg atctcatcga gaaacagcgt gccgttatcc gccatttcaa acttcccgct
    34261 gcggttactg atagcgccag taaacgctcc tttcacatgc ccgaacagtt cactttccgc
    34321 cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg cccgtggcga
    34381 ggcttcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac cgctgatcag
    34441 gacgttgaga tcggacgccg ccacaatctc aatctctttt ttcaattgcg tcatacctgg
    34501 cgacagaccg atcatctgcg tctgtttcac tgcttcaaac ggcgcggcat cgcctggcag
    34561 catattctga ctttccagtt gttcaatcag caacgcattg cttaacgctc ccgccgccag
    34621 cgcggcaatc agccgtaact cttcgtcact gaaaacatcg aactgatcgg gctgcatccc
    34681 gtcgagcgtc aatgcgccga tcaggttttg tccggcaaac aatggcagac caacgcaggc
    34741 gtgaaccttc agactctcct gcccgggaat caaaccgtca tagggatcgg gcaattcgct
    34801 gtctgcggga aagcgcacca cgtccccggc gcgggcaatc gcttccagcc gtggatgccc
    34861 ttccagcgca aagcgtctac cgagtacatc cttcgccagc ccgtcgatgg caagcggaat
    34921 aaactgccgc gagtcataac gtagcaacgc agaggcatca cactccagca cctgacgcag
    34981 cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca attcgatggc
    35041 gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa tttgactata
    35101 gatattgtca tatcgaccat ttgattgata gtcattttga ctactcatta atgagcataa
    35161 ttttatttat agagtaaaaa caatcagata aaaaactggc acgcaatctg caattagcaa
    35221 gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt ggtgaaaaat
    35281 aacattcatt gggttggtca acgtgactgg gaagtgcgtg attttcacgg cacggaatat
    35341 aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa aaacgtgctg
    35401 atcgacaccg ttgaccataa attcagccgc gaatttgtgc agaacctgcg taatgaaatc
    35461 gatctggcgg atatcgatta catcgtgatt aaccatgctg aagaggacca cgccggggcg
    35521 ctgaccgaac tgatggcgca aattcccgat acgccgattt actgtactgc caacgctatc
    35581 gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt gaaaactggc
    35641 gacacactgg atatcggcaa cggcaaacag ctcatttttg tcgaaacgcc aatgctgcac
    35701 tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tgctattcag taacgatgct
    35761 ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca gacggagctt
    35821 ttcgagcagt gccagcgtta ctacgccaat atcctgacgc cgttcagccg cctggtaacg
    35881 ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc gacctctcac
    35941 ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa atgggcggcg
    36001 gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa tacccgcatg
    36061 atggccgatg ctatcgccca gggtattgcg gaaaccgacc cacgcgtggc ggtgaaaatt
    36121 ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca atgtcttccg ctcaaaaggc
    36181 gtgctggtcg gcacttctac gatgaataat gtgatgatgc cgaaaatcgc cgggctggtg
    36241 gaggagatga ctggtttacg cttccgtaac aaacgcgcca gtgctttcgg ctctcacggc
    36301 tggagcggcg gtgcggtgga tcgtctttcc acacgcctgc aggatgcagg tttcgaaatg
    36361 tcgcttagcc tgaaagccaa atggcgaccg gaccaggacg ctctggagtt atgccgtgaa
    36421 cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag cacggtgaat
    36481 acggtggtta aagaagaaac ctctgccaac acgacggctg acctcggccc acggatgcag
    36541 tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg agccaatgca ggacgttgcg
    36601 ccaggaacgc cgtggagtga agtcccggat aacttcctgt gcccggaatg ctctctcggc
    36661 aaagacgtct ttgacgaact ggcatcggag gcaaaatgag caacggcatt gtgatcattg
    36721 gttcgggctt cgccgcccgc caactggtga aaaatattcg caaacaggac gccagtattc
    36781 cattaactct gattgccgcc gacagcatgg atgagtacaa caaaccggat ctcagccatg
    36841 ttatcagcca ggggcaacgt gccgatgacc ttacccgcca gacggcgggt gaatttaccg
    36901 agcagtttaa tctgcacctg tttccgcaca cctgggtgac ggatatcgat gccgaagccc
    36961 atgtggtgaa aagtcagaat aatcagtggc aatacgacaa gttagtgctg gcaaccggag
    37021 ccagcgcctt tgtcccgcca gtgcctgggc gtgagttaat gctgacatta aatagtcagc
    37081 aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg acgcgtattg attgttggcg
    37141 gtggcttgat tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa gcggtcacgc
    37201 taatcgacaa cgctgccagt attctggcat cgttaatgcc accggaagta agcagccgct
    37261 tgcagcatcg gttgacggag attggcgttc atctgctgtt aaaatctcag ttgcaggggc
    37321 tggaaaaaac ggattctggc attctggcaa cgctggaccg ccagcgcagc atcgaagtgg
    37381 atgcggtaat tgccgccacc ggactgcgcc cggaaaccgc cctggcacga cgcgccgggc
    37441 tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat gccgatattt
    37501 atgcgctggg cgattgcacg gaaattaacg gtcaggtatt gccgttcctc cagccgattc
    37561 aacttagcgc gatggtgctg gcaaaaaatc ttctcggcaa taacacgccg ctgaaactcc
    37621 ctgcgatgct ggtgaaaatc aaaacgccag aattaccgct gcatctggca ggcgaaaccc
    37681 agcgtcagga tttacgctgg caaattaata ccgaacgcca gggaatggtg gcgcgcggtg
    37741 ttgatgatgc cgaccagctt cgcgcctttg tggtcagtga ggatcggatg aaagaggcgt
    37801 ttggattgtt gaaaacgttg ccggtgtagg catggttttc tccctcgccc aaagggcgag
    37861 gggactgtcc gcgctctttt tacgcttagt tacgccacat ccgccactat gcagcatgca
    37921 ggatgcgcgc cgcagcaata accccctgcc ccagagacaa gccgccatca cccgccggta
    37981 aactctgcgg aaagagtaat gtgaaatcag cgagataatg cgccaggcgt gtacgcagca
    38041 atcggttatg aataacccca ccgctaaata ccagcgtagt gataccgcgc atcgtggcct
    38101 gctcacgcat caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc cacgcgcgtt
    38161 gattaaccgg tgcctgccag ttcagccact gctgccagaa agtggcgaga tccagttgat
    38221 tgtccaccag cggcatcgtc accggatgcg tcactccgtc gcatgaggct gcgagcgcct
    38281 ccagagcaca agccgcttca ccttcataac ttaacgtggc tggcgcacag cccagtgccg
    38341 ccgccacagc atcgaacaaa cgcccacacg atgacgccag cggcgcgtta attccacgct
    38401 caatggcccg cgccagcacg ctccagtttt gctgttgcac acttgtcgtt tcagggtaat
    38461 tctgccactc cggcacaaag cgcaggcact gcgccagcag gttacgccac ggctgctttg
    38521 cagccaaatc gccccccgga agcgccaccg caggcaagcc gcccaggtgc tcacattcgc
    38581 gatagttcac ccgcaggcat tcgccgcccc acaaagcgcc gttctccccc ataccgatac
    38641 cgtcgagcgt caacgcaatg acatcaccgc catccagcgg ccactgatgc tctgccagac
    38701 tcgccgccgc atgagcatga tgatgcagca ccgtttgcgt cggcagattc atttcgcgtg
    38761 cccactggct ggagacatag cccggatgcg cgtcatgcac aacgtattgc ggagtgaaat
    38821 cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg catctggatg ccatcgtcac
    38881 ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc gcgcaccagg cagaaggtgt
    38941 ttttcagatc cgcgccgaga cacagcacag gcggaacatt tttaaagccc ggaggcaaag
    39001 ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat ttcgccgctt tcgcgcacca
    39061 ccgaatcatc catccgctgc acgatgtcgc ggttatgtag caagaatccg tcggcaatgc
    39121 cctgcaaatc cgccagcgcc tgttcgttgc tgatagccgg tggtttaccg ctcaggttgc
    39181 cggaggtcat caccagcgga cattgcagtt cctgtaacag caaatgctga agcggattcg
    39241 caggcaacat caccccaact tcgttaaggc caggggcgat atcatcacaa agctcaggaa
    39301 cgtatttttt atccaccagc acaatcggcg cggcgggtgt ggtgagcaac tggcgcgcag
    39361 cgtctggtaa accttctgcc actggcaaca tgaccgccag cggtttcgcc gggcgatgtt
    39421 tgcgcgcccg aagtgtcgcc accgcgttac tgttacgtgc atcgcaggca agatgaaatc
    39481 cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata gctgcctgta
    39541 atgccgcctc ttgttccgca tgttcaccat gacttaccca ttcaagatgt gggccacact
    39601 ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg tactctttgt
    39661 cacaagccgg acacaacgga aacgccgcca tcacggtaaa cgggcggtcg taaggcatgg
    39721 cgcgaataat ggtgaaacgc gggccacagt gagtacagtt gataaacgga taacgataac
    39781 gccgctcgcc tggggtattc atttcggcaa gacaggcagg gcaagtagcg gcatcgggaa
    39841 caatttgcgt attcatggtg ccgcctgtgc tctggcgtat agtgaactcg gtgggcagtt
    39901 gtgaccagat aaacggctca cgctcgacgc tatcaatacg cgccagcggc gggcagtgct
    39961 gatgcaactg cacaagaaac gtttccgggt cttccagcag ccggacttct acgccatcgc
    40021 cgtcattaca gacatcgccg tgaagattta attgctgtgc cagctgccag acaaacggac
    40081 gaaaaccgac gccctgcact ttgccacgaa tacgcagttg gacaccgcaa gatgtgtttt
    40141 ttgccattga gttattcccg ccatcatgaa ttgcgcaacc cgccctgccg gacacgacag
    40201 cgtcgtatcc ggcaatcaca ggtcggcgat accgcccgct ccgtattcta cgaatatttc
    40261 cgggaattcc tttgatgcca gaacagttct gtaagatttt tagaacatca gcgccgtacg
    40321 gcggcgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa tcagcgcatg
    40381 ggtcgggcaa gccgccatac acgccgggcc gtcttcacga tggttacaga ggtcgcattt
    40441 attggcttcg gctttgtcag cccgtacatt cagccctgcg ccgctgttgc gaatcaccgg
    40501 acgtaccacc acttccatcg cgccatacgg gcaagccaca acgcaggttt tgcaaccaat
    40561 gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg ctgatagcac catttgggca
    40621 gacgttagcg cacggtgcat cttcacactg acggcagact gttgccgtag aaatgttcac
    40681 acctttaatg acatggattc gcggtaaaaa agtttccggg gtcagcgatg cacagtcctg
    40741 attctcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa tacatttact
    40801 cgcgtcagca atgatgaaac ggttcatcaa attctccagc aatgacagtt aatgcgccga
    40861 tacattcaca aatcatgcca gtttttaatt tactgttatt taaagaaatt aaattctgta
    40921 atgcaggaaa aacgatgtca tcgacactag tgacgatgac atgtgatgac aatgtttatt
    40981 gcgaaagagc aatgagggag ccgcggcgga tcagttttcc gctgaaggtt ttcggcggtg
    41041 agaaatcccc accatcgagc ataaaaatca gccgtccaat aatttcctga atcatctcag
    41101 tcaccggaat ttttacgctg gagagcgccg gaacgatata gggggcaatg gcgatatcat
    41161 cgaatccgat aactgacacc tgctctggca ccgctacgcc gcgctcgtgt aacgctttca
    41221 tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta gccccacgtt
    41281 cgagcaacgt ttctacccct tcggccccgc tggcaggcgt ccatttaccg ttagcgataa
    41341 gtttttcatt gagcgcaata ctatgctgcg ccagcgcgtc tttatacccg gcaagacgtt
    41401 caatgctggt gggagaatcc atcgagccgg taaggaaagc aatctcctga tgtccggcgt
    41461 ttatcaactc tgccacggcg ttaaagctgg tctgtttatg atcgcaccag acgctatggc
    41521 tgctgttttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgt gcgtcaatga
    41581 tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg tcgcagcgca
    41641 aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta ccatctgcca
    41701 atagcaactg tcgccctttc tcttccgcca ttcgcgcggc atgaaagagt aattcactaa
    41761 aataaatgcc gtggtaaagc gtgttggtca cgaccagccc cagcgtctga gtactcttcg
    41821 ccgacagatt gcgcgccagc aagtttgggc ggtaaccgct ctcttctacc gcctgaaaca
    41881 cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg gaaacggtcg
    41941 cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt
    42001 tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc aatcggcggg
    42061 aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg aacaattaac tctcttgctt
    42121 atttagtgac aactattcat gattttgtga aaccggtttc ctaattccgt ttcagcatcg
    42181 gcatttttcc gtcacgtcga ctgataacaa ctacatctac cctactgata acaggataaa
    42241 atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca ctgggcggcg
    42301 ttgataacat ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt atcaaagatg
    42361 accagcttat cgacagtccg acgttgaaaa ccatctccgg cgtgctcggc gtggtacgta
    42421 gtgacaacca gtgccaggtg attatcggca ataccgtttc acaagccttt caggaagtcg
    42481 tcagcctgct gccgggagat atgcagcccg cacagcccgt gggtaaaccc aaacttacgc
    42541 tacgtcgcat tggtgcgggg atcctcgatg cgctgatcgg caccatgtca ccgctgatcc
    42601 cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag atgagcggcg
    42661 tgctgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac ggtgctttct
    42721 tcttcctgcc gctgatggtc gcggcttctg ccgccatcaa atttaaaacc aatatgtcgc
    42781 tggcgattgc catcgcgggt gtgctggtac atccgagttt tattgaatta atggcgaaag
    42841 cggcccaggg tgaacatgtc gaatttgccc tgattccggt caccgcggtg aaatacacct
    42901 acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg
    42961 acagcattac accggcggtg actaaaaact tcctcaagcc aatgctgatt gtgttgattg
    43021 ccgcaccgct ggcaattctg ctgattggcc cgattggtat ctggatcggt agcgccattt
    43081 cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc attatgggcg
    43141 cgctgtggcc tctgctggta atgaccggga tgcaccgcgt ctttacgcca accatcattc
    43201 agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc
    43261 tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg gaactgcgcc
    43321 agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa ccggcgttat
    43381 atggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc ggttttattt
    43441 gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg ccggggctat
    43501 ttaccagcgt gcagttcttc gatccggcga atccaatgag catcgtctgg gtgttcgcgg
    43561 tcatggcgct ggcggtggtg ctgtcgttta tcctcacact gttgctcggc tttgaggata
    43621 ttcctgttga ggaagcgact gccgaggcgc gaaagcatca gagcgcacaa ccgaccgtcg
    43681 ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag gttttttatg
    43741 gggcggcgcg cttgccgcca accagtctga aggtgcgtac cgtgaaggtg gcaaagggct
    43801 gaccaccgtc gatatgatcc cacacggcga gcatcgaatg gcggtgaaac tggggctgga
    43861 aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga cggattttta
    43921 tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaaag tgttccgtac
    43981 ctcaattgcc tggagccgcc tcttcccgca gggcgatgaa ctgacgccca accagcaggg
    44041 cattgctttt taccgttctg tctttgaaga gtgtaaaaag tacggtatcg aaccgctggt
    44101 cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct cctggcgtaa
    44161 ccgcaagctg gtggagtttt tcagccgcta cgccagaatc tgctttgaag catttgatgg
    44221 tctggtgaaa tactggctaa ccttcaatga aatcaacatt atgttgcata gcccgttctc
    44281 cggcgcgggt ctggtgtttg aagaaggcga aaatcaggat caggtgaaat atcaggctgc
    44341 gcatcaccaa ctgattgcca gtgcattagc caccaaaatc gcccatgagg ttaacccgca
    44401 aaatcaggtg ggctgtatgc tggcgggcgg taacttctac ccttacagtt gcaagccgga
    44461 agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg atgtgcaggc
    44521 gcggggcgcg tatccggctt actctgcccg cgtattccgc gaaaaagggg taaccatcga
    44581 caaagcaccg ggcgatgatg agatcctgaa aaacaccgtc gattttgtct ctttcagcta
    44641 ttacgcctca cgctgcgcct cggcggagat gaacgccaac aatagcagtg cggcgaacgt
    44701 agtgaaatcg ctgcgtaacc cgtatctgca agtgagcgac tggggctggg gtattgatcc
    44761 actcggtctg cgtatcacca tgaatatgat gtacgaccgt tatcagaagc cactgtttct
    44821 ggtggaaaac ggcctcggcg caaaagatga atttgctgcc aatggtgaga ttaacgatga
    44881 ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga ttgcagatgg
    44941 cattccgctg atgggctaca ccacatgggg ctgtattgat ttagtttccg cctctacggg
    45001 tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt gacgacgtag gcaacggcac
    45061 gctgacgcgc acacgtaaga aatcgttctg gtggtataaa aaagtaattg ccagtaatgg
    45121 tgaagattta gagtaagtaa ctaccgtcgc acgaaccagg aaagcccgat aagcgttgcg
    45181 catcgggcaa tcttgggttt ggcatcagtc aaaatcaaag aaaccattcc ggcttaacgc
    45241 caaatcgggc tgaaagtgcc ttaatatggg atatcgtcag ggatcgttgc ccagacaaaa
    45301 tctgactgac cagagattta gagccgattt cgttcttcaa atccgcatag gtcaaattat
    45361 gttgatcaat cagcgtacga agtaaggcca cgccaaccgg cattgctgca acggctttgt
    45421 cgaactctgc aaacttttcg ttgttgtttt catattctgc aatgcggctc gccagaaagt
    45481 cgataagtgg attggtgtca tcttcttcaa tcaaatattc gaccagttcc agtgcttcac
    45541 gataatcatc ttctgagtca ctgcctccca gaaaggggac tgcattgacc agttctcttg
    45601 ttgctttaac agcgcgtgca gcattagcag tcattctttg ttctctctgt agtaacgtgt
    45661 cagtttgtca tactctgcgt gattcgttat gtgcttcaca aagaaccgct tattaacaaa
    45721 gtttatgtag gcaataaccc taagattgtt cccgccgaca tccagaacgt accatttgtt
    45781 gcgatattta aaattgtcga ggttaggaaa cgccgttcgc atttcttctg gtgtagaaaa
    45841 atccgtttcc ttaataaccc gatacaatga gtgtagagcc agagcgtcgt taggatattt
    45901 gcgcgcacac tcttcaaatg gcgctttcga aatgatgtgc attttaactt ccttattagc
    45961 gcctgaatga ctaaacctgg actaccattc cttcggattt atttttctat agtagttgat
    46021 cacacgggtg ttcacaatat gtaaacaccc gtctacacgt cacaattgca cgtccaaatt
    46081 ccaaaacgtg aacatgtttg tctacgaact actcttcttc caccgctaac tgcgcgaatc
    46141 cgccattccc ttcccagcct tccagtcgtt gataaacggt ttctacggcg tctttaatcg
    46201 gctgggtcat cgggtagtaa aagccgacga tatccggctg gatgccgagg aaaatcactt
    46261 cgccaatgtc ttctttcaac tggtcgataa ggtagttcaa cggcatgtta tgggtagtca
    46321 tcataaacat ctcggcgata tcatccggat cgatgatgcg gatctcgccg gggtttagcc
    46381 ccatatccgt ggcgtcgaca atcaacagtc gtgtcgggcg cagttcgcga atagcgacga
    46441 tgtcgttttc cggtgcgcta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg
    46501 cacacttttc cgccagcagc ggacctgcgc catcatcgcc catcatgcta ttgccaacac
    46561 agagtaaaac gtcagtcacg caatctcctc accatcaaat agatggcgtt ttcctgatga
    46621 atatcatgca gcatacttaa cagcattgta ctccattctt tctgctgcga agtttgcgca
    46681 gcccgggctt tatcgaatgc gttggccagc atcggcacat ggttaatgtc gatgacgatc
    46741 tcaccatatt tcggcacgcc ttccattttg cggcgggctt cgctgcccgc ctccagagtg
    46801 gcaatccatg ccagatattc atcccacgga caggtgagcg ccgcttccag gcaatcgata
    46861 accccaaggt ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc
    46921 gtggcatcgt tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc
    46981 atcggatacg cgcctcttca acaacatgat tcagatggct gacaatctcg ttcagacgcg
    47041 gatcgttttc cgcttccagc cagcgtgcaa cctgctcttc gccctgccct aactgtgtca
    47101 ggaagtcatc ggcaatctgg cgaccgtaac ggtaacccgc caggcggcga gcttcgcgat
    47161 ccactttcac gcgcagcggc tgcaccatat cgccatgcag gatctccgcc ggttgttcat
    47221 cctgctcacc cggcccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa
    47281 agccgtacag cgtggcggca ggcgttggcg ggcagccagg gatataaaca tccaccggga
    47341 cgattttatc cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccgctgttac
    47401 cgcaggcacc gtaggagata caaattttcg ggtccggcgc ggactgccac gcacgcagcg
    47461 caggggatcg cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg
    47521 gtgaaggaac gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgaa agcgtggcga
    47581 aaatttcgat ttcgcagccg ttgcagccgc cgcagtccac gcggtaaacg taggcagaac
    47641 gtttgatttt tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatcg
    47701 ggaccggaat gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt
    47761 catatggcga gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc
    47821 cgggcaagtc tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttgag
    47881 cagcgcaatg gcgtagtcga tctctttctg gacggcgaaa ggacgattgc agacgcgaca
    47941 gttgcacagc gcgaagcggg actgttgcag gaagtcttct ttcttccaca ccgccagttc
    48001 gtactcttgc gacagcttaa tcgccgccgt cgggcaaact tcttcgcagc gtccacagaa
    48061 gatgcagcgc ccgagattaa actcccaggc aagctctcct gtggcgaggt cagtttcaac
    48121 cgttaaggcg tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg
    48181 gttctgctct ggcttaccac ggaagttttt atcaaccgca atcggctcca gcggataaga
    48241 cgaggtcgcc gtgccggttt tgataacttt tttgataaag gtaaacatgg cgattcctta
    48301 tttcagcggc gagtttttac gctcaatgct gtaacgctcg agttctttgt acggcaccac
    48361 tttgctcttc ttcttacgca catcgaccac ggtcatgcgg tcggtacagg agtagcaagg
    48421 gtccaggcta ccgataatca gcggcgcatc ggaaacggtg ttgccgcgca gcatgtagcg
    48481 cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt
    48541 gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc gcttcggcaa agcccagcgc
    48601 aaaacggtgc ggaatatagg taaagccttc caccatcagc ggaccgcccg gcaggttatc
    48661 cagaccgtag tcgatcatgt tcagcgcggt atagacctcg ttgatacgca ctttcagacg
    48721 ggaaataacg tcgcagccct gctcgctgtg gacttccatt ggcagcaggc cataaccaac
    48781 aaacgggtga tcggcacggg tatcgcgggc gtgaccgctg gcgcggacca tcgggccaac
    48841 gttactgaaa tcgcgggcga tttccgggtc cagacgacca atgccgacag tacgctgttc
    48901 catgttcggt gtgctcagca gcacatccac cagctcctgc acttcacgac gcatctgttg
    48961 cgccaactgg cgggtctgga tcatgtcatc tttcagcaga tcgcgacgaa ttccgccgat
    49021 caggttcagg ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt tcatggaggt
    49081 ttcacgcacg cggaagaact gcataaagcc ggagtcgaag ccggtaaagt gacaggcgag
    49141 gccgaggttg agcagatgcg agtgcaggcg ttctacctcc agcagaatgg cgcggatcat
    49201 ctgcgcacgt tctggcacct gaatacccat cgcgttttcc accgacgtgg tgtaggcggt
    49261 gctgtgagca aagccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttgta
    49321 acccatgcgg gtttccgcca gtttttccat gccgcgatgg acatagaaca ggcggtagtc
    49381 ggcgtcgata atgttttcgc catcgacgaa cagacggaag tgacccggtt cgtcggaagt
    49441 gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa
    49501 ctcgtaggtt tcagcatcgg tagtcggtgc cggacgttga cgataatcca tgctgtcttt
    49561 acgcagcgga taaagttcat ccggccagtc atccggcagc accagacgac gttcatccgg
    49621 cagccctacc ggaatcaaac cgtacatatc gcgcacttca cgctcgcccc ataccgccgc
    49681 cggaacgcgc ggcgttacgg acggatattc cggtttgttg gcgtcaactt caacgcgcac
    49741 ggttatccag catttggtgc ccttctccat cgacagcacg tagtaaacgg cgtaatgacc
    49801 attaagtttg cgttcgtcgt taccaaacag caccgacagc cagcccccct gcttgtagta
    49861 gagaaactcc accacttccg gcaggtagtt caccttcacg gtcacggtca gctgatcttt
    49921 ggtctgccag gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cagcgagata
    49981 atgttgacct aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag
    50041 gagacgaacg ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg agtaatatca
    50101 agacgcagac gggccatgct gttttcgaac agcgcgataa ccaggacgcc aaccaccagt
    50161 ttgacgatgg caatcaccag cgccagcagc agcccaccga cggtgaaggt ttccatttgt
    50221 ccccacggaa taaacacccc gacgaacatc tgcaacacca ccagctgttt caggctgata
    50281 ccccatttca tgacgccaaa gccgctgccg ctatactcag agagcggacc ttcctgcaac
    50341 tcctgctcgg cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaat
    50401 gcacaggcac aaagcgccag caccagcgga atgctctggc tcagcggcca gtgataaacg
    50461 gtgtcggtga tgttgctgat gttggtggaa ccggcaacct gtgcggcgac ccacagacca
    50521 agcagcagca tcggttcaac cagcacgcca agcatcgctt cacggctcgc gccgatagcg
    50581 gtaaacgggc taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag
    50641 agatacagta aagtgatcaa atcgcccagc tgcggcagcg gagaaccaac ggtcaccacc
    50701 ggcagcgcag tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac
    50761 acccagccgg aggcatccgg gccaacgctc tggcggccca gcagtttgat aatgtcgcga
    50821 tactcctgca acacgcccgg cccgcgacgg ttatgcaagc gggcgcgcgc cacgcgggtt
    50881 ataccggaga gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa
    50941 acactcattc tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca
    51001 gcgccatccg gcggaacagc aacgcactcc cctcgcactg ccagcccggt accagcgaca
    51061 ccggattcag ccatttgcgc agtttcagca ccggcgcaaa cgcctgtttc accggcatgg
    51121 caaaaccgtg agcagtaatc accattgatt tttcatggtc gtagccgcac acccaggccg
    51181 cgccgcggga acgcgacggc aaacgatcgc ctttgcaaat cgccataatg atgaatggca
    51241 gcagcgggca ggcaatcagc agcaacgtga tcatgggttg agaaacggtg gtgttagcag
    51301 gctccagcgg cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac
    51361 cgccaattac gcagcaaatc gccagtgcca ccacgcttac gctcatcagg agcggcgcac
    51421 tggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt
    51481 tcgccataca catcaccgcc agcgcaccgg taattgccag acccactgcg agcagcggcc
    51541 ccagcagacg ggcaacaaac gcgccactat tgctcagttt gaagaaggat tgatagataa
    51601 cccattcccc ggcaaacccg ttcagcggcg gcaacgcagc cattgccatc agcccgacta
    51661 acatggcgat ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc
    51721 gatgaccggt acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc
    51781 tatggttaag cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt
    51841 cgagcgcgat acccgttacg ccagcgccca gccccagcag gataatgccg atattctcca
    51901 gggtgtggta agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga
    51961 cgaacgcggt gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac
    52021 cgcccagcag cgacagggtt aaaatgccca gcaggccaat tttcatgact accgtagaaa
    52081 acaacgcggc agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg
    52141 gaataatccc ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga
    52201 gcggcagctg ttgcatacgc atatccagca ggcgcagatc cagcgtgccg taacgctgcc
    52261 acagcagcca gcaggcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca
    52321 gtttgccctc tttgctgttg ctggtgagga acaccgcaca cagggccatg atttcggcca
    52381 ttaccacgaa catgccgagg ttgctggcga tgacggcaca gacggcggca gccatcaaca
    52441 tattgatctg caagccgttg catttcacct gcgcgtggcg atgccagtca atgttgtaga
    52501 ggctgacaaa cagaccgcac aggccgagcg taatcagcca aatcgcgtta agcggagaga
    52561 tttgcacatc gtagcttacc agcgacagcg caccgctcac gccaaccgca ccagtcagca
    52621 cagtgaagcc cgcggctgcc gtatacaggc tacccaccgc gccgccaatt ccggctatcc
    52681 agccgcttag cgccttttga aaagaaaaga gaaatgccag aacagcggcg gcggcaaacc
    52741 acgccacgcc actattgatc agggaaattg cgctcattta gcctctccac tttgagcctg
    52801 ctgaaacaag gtgagatcgc caaagtccgt gttaaaagtc agctcacgct tacgtttgct
    52861 gacgcgggcg atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac
    52921 gcaggccgga ccttgttcat caaagctaca aaggtcacat ttgacggcga tcgcgcgaat
    52981 acctggcacc cagtcaagca atgtgctgac acgcgccgga gcaggcggtg ccggtggcgc
    53041 tttcggggta ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc
    53101 gccaaacggg caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg
    53161 cacggcccca tcaacgcggg tgatggcgtt aaccgggcaa accaccgcac agggcgcatc
    53221 ttcacagtgg tgacagagct gcggcgcaga ttctttttca ttgagcatca ctcgcaggcg
    53281 cggcattgat tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaggtgtg
    53341 gcagccgata cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg
    53401 tgattgtcat ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac
    53461 gcccatcccc aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg
    53521 ggctttgtcc ctttaccagt tggcttaaat tcaccggcac attgttttca atggcggtgt
    53581 ataagctgtc gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat
    53641 cgcggatgtt gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc
    53701 gctgtttgtc ttcaccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc
    53761 catccgccag ggtgacgtaa atgcgaaaat gttcaaaaag gacgaaacag agttctttgt
    53821 ccggcgcgca gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca
    53881 gcgaattgca gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat
    53941 cggctttctc gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc
    54001 agctttaagc attttctgtg ccaactttta attcattgtt atgaaacaga tttttaagat
    54061 aaagatgaat ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg
    54121 cagggataat caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg
    54181 atatgcacga aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa
    54241 aacacggcgc aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg
    54301 aaaccagctc tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt
    54361 gtaaactgca cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg
    54421 tgacgctact gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga
    54481 ttgtggcaga cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt
    54541 gtacaacatg cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc
    54601 attccgcgtt tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg
    54661 gcattaaagc gcctgaattt acccccagcc agactgaaga aggcgatctg cattacggtc
    54721 atggcgaagc gggtactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa
    54781 ttgacgtgct ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc
    54841 gcaagcaact ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga
    54901 cggaaacctt aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggcgaccagc
    54961 aaaccgtgaa cgatgccgcg cgcattcgcg ctaccggcac accagcgatt caggtgaata
    55021 ccggtaaagg ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgcctgccac
    55081 tggacgataa cggtattctg tttatcgaaa acgtcggcaa cctcgtgtgc ccggccagct
    55141 tcgatctcgg tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac
    55201 cgctgaaata tccgcatatg tttgccgcag cttcgctgat gctgctcaac aaagttgact
    55261 tgctgccgta tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc
    55321 cagaaattga aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga
    55381 actggctgga gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg
    55441 caaccaggcg aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg
    55501 cagctgcgat gaaaacggtc agccacgcgt aggccagtgg gtactggtcc acgttggctt
    55561 tgccatgagc gtaattaatg aagccgaagc gcgcgacact ctcgacgcct tacaaaacat
    55621 gtttgacgtt gagccggatg tcggtgcgct gttgtatggc gaggaaaaat aatgcgtttt
    55681 gttgatgaat atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt
    55741 gcttcacatc tctcttacac agccgaacgc cctctgcgga ttatggaagt gtgtggcggt
    55801 catacccacg ctatctttaa attcggcctc gaccagttac tgccggaaaa cgttgagttt
    55861 atccacggtc cgggttgtcc ggtgtgtgta ctgccgatgg gtagaatcga tacctgcgtg
    55921 gagattgcca gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg
    55981 gggaaacagg gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac
    56041 tcgccgatgg atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc
    56101 ggcttaggtt ttgaaaccac catgccgacg acggccatca ctctgcaaca ggcgaaagcg
    56161 cgtgatgtgc agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc
    56221 agtttgctgg aagagccgga taacggtatc gacgcgtttc ttgcgcccgg tcacgttagt
    56281 atggttatcg gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg
    56341 gtcgctggtt tcgaacccct tgatctactg caaggcgtgg tgatgctggt ggagcagaaa
    56401 atagcggccc acagcaaggt agagaatcag tatcgtcggg tggtgccgga tgccggtaac
    56461 ctgctggcgc aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc
    56521 ggcttaggcg tgattgaatc ttctggtgta cacctgacgc cggattatca acgattcgat
    56581 gccgaagcgc atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcacgttgt
    56641 ggcgaagtat taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt
    56701 aatcctcaaa ccgcgtttgg tgcgctgatg gtttcctccg aaggagcgtg cgccgcgtgg
    56761 tatcagtatc gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggtagcg
    56821 gcggccaggc gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt
    56881 ggctggcaga gcaggaagat caggcacgtc ttgatctggc gcagctggta gcggaaggcg
    56941 accgtctggc gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta
    57001 atatcggcaa gctggcgatt tgcggaaccg cgaatgacgt tgcggtcagt ggcgctattc
    57061 cgcgctatct ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag
    57121 ccgtagtgac cagcatggca gaaaccgccc gcgcggcagg cattgccatc gtcactggcg
    57181 atactaaagt ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg
    57241 gcgcaattcc ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattgc
    57301 tggttagcgg tacactcggc gaccacgggg cgactatcct taacctgcgt gagcagctgg
    57361 ggctggatgg cgaactggtc agcgactgtg cggtactaac gccgcttatt cagacgctgc
    57421 gtgacattcc cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag
    57481 ttcatgagtt cgcggcagcc tgcggttgcg gtattgaaat ttcagaagcg gcactgccgg
    57541 ttaaacctgc tgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca
    57601 acgaaggcaa actggtgatc gccgttgaac gcaacgcggc agagcaagtt ttggcagcgt
    57661 tacattccca tccactgggg aaagacgcgg cgctgattgg tgaagtggtg gaacgtaaag
    57721 gtgttcgtct tgccggtctg tatggcgtga aacgaaccct cgatttacca cacgccgaac
    57781 cgcttccgcg tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg
    57841 caccgctttg cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc
    57901 ggacaacaag ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg
    57961 ctgtgtgagg ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt
    58021 gtgttgtggc aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc
    58081 cccattaaat atgaagacga aactgttctg gcacacggtc cggtacgcag cattttgtcg
    58141 cgccctgata cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggcc
    58201 gcaggtgggc tataccccaa atttggtcac tattgcctga tgccactggc ggcggaaggg
    58261 catatttttg gtggctgtga atttattcgt tatgacgatc gcccctggag cgaaaaagag
    58321 ttcaatcgtc tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc
    58381 cgcgtcgtca ataatgtcga ctatgagttg ttatgccggg agcgcgataa cttccgcatc
    58441 ctggttgcca ttaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa
    58501 gtcgccaaag aaatccatta ctacttcgac attgacgata tcagcatcgt cttacgcagc
    58561 caccgtaaaa acaaactcaa catctactcc actcactatc ttgataaaca gcatcccgcc
    58621 cacgaacaga gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagcaaa
    58681 gagatgctgc tgattaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg
    58741 ttcgacacct ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac
    58801 accatgctgg gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat
    58861 ctgaatttac tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc
    58921 tatcaggaaa tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag
    58981 cagctcaaca atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac
    59041 agcgtactta aacaagttga aatggtggcg caaagcgaca gtaccgtgct gattctcggt
    59101 gaaactggta caggtaaaga gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat
    59161 aatcgccgca tggtaaaaat gaactgcgcg gcaatgcctg ccggattgct ggagagcgat
    59221 ctgtttggtc atgagcgtgg ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt
    59281 gaactggcgg ataaaagctc cctgttcctc gacgaagtgg gcgatatgcc actggagtta
    59341 cagccgaagt tgctgcgtgt attacaggaa caggagtttg aacgcctcgg cagcaacaaa
    59401 atcattcaga cggacgtacg tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc
    59461 gccgaccgtg agttccgtag cgatctctat taccgcctga atgtattccc gatccacctg
    59521 ccgccactac gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa
    59581 attgcccgtc gtctcgggcg caatatcgac agcattcctg ccgagacgct gcgcaccttg
    59641 agcaacatgg agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcggta
    59701 ttgctaacac gcggcaacgt gctgcagctg tcactgccag atattgcttt accggagcct
    59761 gaaacgccgc ctgccgcaac ggttgtcgcc caggagggcg aagatgaata tcagttgatt
    59821 gttcgcgtgc tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt
    59881 ctggggctga aacgcacgac cctgctgtca cggatgaagc gactgggaat tgataaatcg
    59941 gcattgattt aacagatgtg gttatactcg atcaacgttc aatgaaagga ggcagtatgg
    60001 cagtttcagc aagaaatcaa ttaaccggaa cagttagcgc agtagcaatg ggcgctgtga
    60061 atgatgaagt agaactgacg ctggcaggtg gtgcaaagct ggtggcaatt gtgactcaca
    60121 gcagccagca agcgctgggt ctggcgaaag ggaaagaagc gattgccctg attaaagccc
    60181 cgtgggtaac gctggcaacg gaagactgtg gactgaaatt ctctgctcgc aaccagtttg
    60241 ctggcagcgt gtcgacaatc accgaaggcg cagtgaacgc cactgtccat atcaaaacgg
    60301 atgcgggttt tgaaattgtg gcagtggtaa caaatgaaag tcaggatgaa atgaaactca
    60361 ccactggctc gcgcgttatt gcattgatca aagcatcagc catcctgatt gccaccaaag
    60421 cgtaagcgcc ttatttgccg gagagtatgg ctctccggca gatttatttc tttggacgat
    60481 atttttctgg caactccggc accggacgct tgtcatcgat gagatgacgc acggttaaga
    60541 tcggatgacg ccacagcatt cgcggcccag cccaacgcat aatctgtttc atctcttcac
    60601 gctttgcagg ctggtaacag tgcaccggac actgcttaca ggctggtttc tcttcgccga
    60661 acacacattt atccagccgc ttttgcgcgt aagtaaacaa cgtctcataa tgctccggct
    60721 ccgctgacgc ctgcgggcat ttcgcttgat aaagatcgat cattttttta atcgtcagtt
    60781 tttcacgaga gatacgcttg ccggacatgc tgcctccatc tcattaagat gcatttatat
    60841 tacaacttaa tcttaaaggg cactatgact ctaaagaaga agggttagcc aaccgataca
    60901 attttgcgta cttgcttcat aagcatcacg caaaagctgc aaaacagcat ctttcccgga
    60961 accagcatca agaactcgcc gttcgcttct tcccctgaaa tgattaactc cggtatcatg
    61021 tgcgccttat gtgattacaa cgaaaataaa aaccatcaca ccccatttaa tatcagggaa
    61081 ccggacataa ccccatgagt gcaatagaaa atttcgatgc ccatacgccc atgatgcagc
    61141 agtatctcaa gctgaaagcc cagcatcccg agatcctgct gttctatcgg atgggtgact
    61201 tttacgaact gttttatgac gacgcgaaac gcgcctcgca gctgctggat atttcactga
    61261 ccaaacgcgg tgcttccgcg ggagagccga tcccgatggc ggggatcccc taccatgcgg
    61321 tggaaaacta cctcgccaaa ctggtgaatc agggcgagtc cgttgccatc tgcgaacaaa
    61381 ttggcgatcc ggcgaccagc aaaggtccgg ttgagcgcaa agttgtgcgt atcgttacgc
    61441 caggcaccat cagcgatgaa gccctgttgc aggagcgtca ggacaacctg ctggcggcta
    61501 tctggcagga cagcaaaggt ttcggctacg cgacgctgga tatcagttcc ggtcgttttc
    61561 gcctgagcga accggctgac cgcgaaacga tggcggcaga actgcaacgc actaatccgg
    61621 cggaactgct gtatgcagaa gattttgctg aaatgtcgtt aattgaaggc cgtcgcggcc
    61681 tgcgccgtcg cccgctgtgg gagtttgaaa tcgacaccgc gcgccagcag ttgaatctgc
    61741 aatttggcac ccgcgatctg gtcggttttg gcgtcgagaa cgcgccgcgc ggactttgtg
    61801 ctgccggttg tctgttgcag tatgcgaaag atacccaacg tacgaccctg ccgcatattc
    61861 gttctatcac catggaacgt gagcaggaca gcatcattat ggatgccgcg acacgtcgta
    61921 acctggaaat tactcagaac ctggcgggcg gtgcggaaaa cacgctggct tcagtgctcg
    61981 actgcactgt aacgccaatg ggtagtcgta tgctgaaacg ctggctgcat atgccagtgc
    62041 gcgatacccg cgtgttgctt gagcgccagc aaactattgg cgcattgcag gatttcaccg
    62101 ccgagctaca gccggtgctg cgtcaggtcg gcgacctgga acgtattctg gcacgtctgg
    62161 ctttacgaac tgctcgccca cgcgatctgg cccgtatgcg tcacgctttc cagcaactgc
    62221 cggagctgcg tgcgcagtta gaaaatgtcg atagcgctcc ggtacaggcg ctgcgtgaga
    62281 agatgggtga gtttgccgaa ctgcgcgatc tgctggagcg ggccattatc gacacaccgc
    62341 cggtactggt gcgcgacggt ggtgttatcg catcgggcta taacgaagag ctggatgagt
    62401 ggcgcgcgct ggctgacggc gcgaccgatt atctggagcg tctggaagtc cgcgagcgtg
    62461 aacgtaccgg cctggacacg ctgaaagtcg gatttaacgc ggtgcatggt tattacattc
    62521 agatcagccg tggacaaagc catctggcac cgattaacta catgcgccgc cagacgctga
    62581 aaaacgccga acgctacatt attccagagc taaaagagta cgaagacaaa gttctcactt
    62641 caaaaggcaa agcactggca ctggaaaaac agctttatga agagttgttc gacctgctgt
    62701 tgccgcatct ggaagcgttg caacagagcg cgagcgcgct ggcggaactc gacgtgctgg
    62761 tgaacctggc agaacgggcc tatacgctga actacacctg cccgaccttt attgataaac
    62821 cgggtattcg catcaccgaa ggccgccatc cggtggttga acaggtgctg aatgagccgt
    62881 ttatcgccaa cccgctgaac ctgtcgccgc agcgtcgcat gttgatcatt accggtccga
    62941 atatgggcgg taaaagtacc tatatgcgcc agaccgcact gattgcgctg atggcctaca
    63001 tcggcagcta tgtaccggca caaaaagtcg agattggacc tatcgatcgc atctttaccc
    63061 gcgtaggggc agcggatgat ctggcatccg ggcgttcaac ctttatggtg gaaatgaccg
    63121 aaaccgccaa tattttacat aacgccaccg agtacagtct ggtgctgatg gatgagattg
    63181 ggcgcgggac gtccacttac gatggtctgt cgctggcgtg ggcgtgcgcg gaaaatctgg
    63241 cgaataagat taaagcgttg acactgtttg ccacccacta tttcgagctg acccagttac
    63301 cggagaaaat ggaaggcgtc gccaacgtgc atctcgatgc gctggagcac ggcgacacca
    63361 ttgcctttat gcacagcgtg caggatggcg cggcgagcaa aagctacggc ctggcggttg
    63421 cagctctggc cggcgtgcca aaagaggtta ttaagcgcgc acggcaaaaa ctgcgtgagt
    63481 tggaaagcat ttcgccgaac gccgccgcta cgcaagtgga tggtacgcaa atgtctttgc
    63541 tgtcagtgcc agaagaaacg tcgcctgcgg tcgaagcact ggaaaatctc gacccggatt
    63601 cactgactcc gcgtcaggcg ctggagtgga tttatcgctt gaagagtctg gtgtaataac
    63661 aattcccgat agtcttttgc tatcgggaat attaacgaca acagacgaat caaataaaaa
    63721 caccctgtat aataggaaat cttattttac agggtaaaac catgccatct acacgctatc
    63781 aaaaaattaa tgcccatcac tatcgttata tatgggccgt tggtgatatt catggtgact
    63841 atcaattatt acaatcccgc tt
//
LOCUS       DARNVM010000006        62646 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.006, whole genome shotgun sequence.
ACCESSION   DARNVM010000006 DARNVM010000000
VERSION     DARNVM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.006"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            45..1076
                     /gene="ynaI"
                     /locus_tag="SI621_000363"
     CDS             45..1076
                     /gene="ynaI"
                     /locus_tag="SI621_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HEI4063120.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(1127..2740)
                     /gene="mppA"
                     /locus_tag="SI621_000364"
     CDS             complement(1127..2740)
                     /gene="mppA"
                     /locus_tag="SI621_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HEI4063121.1"
                     /translation="MKHSVSVTCCALLISSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3077..3976)
                     /gene="pgrR"
                     /locus_tag="SI621_000365"
     CDS             complement(3077..3976)
                     /gene="pgrR"
                     /locus_tag="SI621_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HEI4063122.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            4114..5034
                     /locus_tag="SI621_000366"
                     /pseudo
     CDS             4114..5034
                     /locus_tag="SI621_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            5105..5743
                     /locus_tag="SI621_000367"
     CDS             5105..5743
                     /locus_tag="SI621_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4063123.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            6034..6762
                     /gene="mpaA"
                     /locus_tag="SI621_000368"
     CDS             6034..6762
                     /gene="mpaA"
                     /locus_tag="SI621_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HEI4063124.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(6737..7702)
                     /gene="ycjG"
                     /locus_tag="SI621_000369"
     CDS             complement(6737..7702)
                     /gene="ycjG"
                     /locus_tag="SI621_000369"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HEI4063125.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEDGVKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            7821..8327
                     /gene="tpx"
                     /locus_tag="SI621_000370"
     CDS             7821..8327
                     /gene="tpx"
                     /locus_tag="SI621_000370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEI4063126.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(8371..9912)
                     /gene="tyrR"
                     /locus_tag="SI621_000371"
     CDS             complement(8371..9912)
                     /gene="tyrR"
                     /locus_tag="SI621_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HEI4063127.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10060..11121)
                     /locus_tag="SI621_000372"
     CDS             complement(10060..11121)
                     /locus_tag="SI621_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEI4063128.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRVQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11118..12515)
                     /locus_tag="SI621_000373"
     CDS             complement(11118..12515)
                     /locus_tag="SI621_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEI4063129.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            complement(12634..12948)
                     /gene="pspE"
                     /locus_tag="SI621_000374"
     CDS             complement(12634..12948)
                     /gene="pspE"
                     /locus_tag="SI621_000374"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HEI4063130.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(13023..13244)
                     /gene="pspD"
                     /locus_tag="SI621_000375"
     CDS             complement(13023..13244)
                     /gene="pspD"
                     /locus_tag="SI621_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HEI4063131.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(13253..13612)
                     /gene="pspC"
                     /locus_tag="SI621_000376"
     CDS             complement(13253..13612)
                     /gene="pspC"
                     /locus_tag="SI621_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HEI4063132.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(13612..13836)
                     /gene="pspB"
                     /locus_tag="SI621_000377"
     CDS             complement(13612..13836)
                     /gene="pspB"
                     /locus_tag="SI621_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEI4063133.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(13890..14558)
                     /gene="pspA"
                     /locus_tag="SI621_000378"
     CDS             complement(13890..14558)
                     /gene="pspA"
                     /locus_tag="SI621_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEI4063134.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDNGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            14725..15702
                     /gene="pspF"
                     /locus_tag="SI621_000379"
     CDS             14725..15702
                     /gene="pspF"
                     /locus_tag="SI621_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEI4063135.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(15822..17087)
                     /gene="puuE"
                     /locus_tag="SI621_000380"
     CDS             complement(15822..17087)
                     /gene="puuE"
                     /locus_tag="SI621_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HEI4063136.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDDHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQCLTNTL
                     IDAKESVPSIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(17125..18405)
                     /gene="puuB"
                     /locus_tag="SI621_000381"
     CDS             complement(17125..18405)
                     /gene="puuB"
                     /locus_tag="SI621_000381"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HEI4063137.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(18407..19894)
                     /gene="puuC"
                     /locus_tag="SI621_000382"
     CDS             complement(18407..19894)
                     /gene="puuC"
                     /locus_tag="SI621_000382"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HEI4063138.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADFMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYTEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLDLLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(20044..20601)
                     /gene="puuR"
                     /locus_tag="SI621_000383"
     CDS             complement(20044..20601)
                     /gene="puuR"
                     /locus_tag="SI621_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HEI4063139.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(20628..21392)
                     /gene="puuD"
                     /locus_tag="SI621_000384"
     CDS             complement(20628..21392)
                     /gene="puuD"
                     /locus_tag="SI621_000384"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HEI4063140.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVL
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            21604..23022
                     /gene="puuA"
                     /locus_tag="SI621_000385"
     CDS             21604..23022
                     /gene="puuA"
                     /locus_tag="SI621_000385"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HEI4063141.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            23171..23335
                     /locus_tag="SI621_000386"
     CDS             23171..23335
                     /locus_tag="SI621_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063142.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            23325..24710
                     /gene="puuP"
                     /locus_tag="SI621_000387"
     CDS             23325..24710
                     /gene="puuP"
                     /locus_tag="SI621_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HEI4063143.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRAPVSET"
     gene            24844..25089
                     /locus_tag="SI621_000388"
     CDS             24844..25089
                     /locus_tag="SI621_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HEI4063144.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            25402..27045
                     /gene="sapA"
                     /locus_tag="SI621_000389"
     CDS             25402..27045
                     /gene="sapA"
                     /locus_tag="SI621_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HEI4063145.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKPDEVKKP"
     gene            27042..28007
                     /gene="sapB"
                     /locus_tag="SI621_000390"
     CDS             27042..28007
                     /gene="sapB"
                     /locus_tag="SI621_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HEI4063146.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITDFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            27994..28884
                     /gene="sapC"
                     /locus_tag="SI621_000391"
     CDS             27994..28884
                     /gene="sapC"
                     /locus_tag="SI621_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HEI4063147.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLILGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            28884..29876
                     /gene="sapD"
                     /locus_tag="SI621_000392"
     CDS             28884..29876
                     /gene="sapD"
                     /locus_tag="SI621_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HEI4063148.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            29878..30684
                     /gene="sapF"
                     /locus_tag="SI621_000393"
     CDS             29878..30684
                     /gene="sapF"
                     /locus_tag="SI621_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HEI4063149.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(30733..31896)
                     /locus_tag="SI621_000394"
     CDS             complement(30733..31896)
                     /locus_tag="SI621_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEI4063150.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSGVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(31905..33278)
                     /locus_tag="SI621_000395"
     CDS             complement(31905..33278)
                     /locus_tag="SI621_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEI4063151.1"
                     /translation="MLRRSLIFLVLLSAGCVSLEPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTHVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSEALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQALLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(33282..36389)
                     /locus_tag="SI621_000396"
     CDS             complement(33282..36389)
                     /locus_tag="SI621_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEI4063152.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSTPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(36389..37510)
                     /locus_tag="SI621_000397"
     CDS             complement(36389..37510)
                     /locus_tag="SI621_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEI4063153.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTVRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(37659..38225)
                     /locus_tag="SI621_000398"
     CDS             complement(37659..38225)
                     /locus_tag="SI621_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEI4063154.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            38703..39491
                     /gene="fabI"
                     /locus_tag="SI621_000399"
     CDS             38703..39491
                     /gene="fabI"
                     /locus_tag="SI621_000399"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEI4063155.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELAKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            39635..40762
                     /locus_tag="SI621_000400"
     CDS             39635..40762
                     /locus_tag="SI621_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HEI4063156.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTTELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALICARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            40830..42764
                     /gene="rnb"
                     /locus_tag="SI621_000401"
     CDS             40830..42764
                     /gene="rnb"
                     /locus_tag="SI621_000401"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HEI4063157.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHIDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(42841..42945)
                     /locus_tag="SI621_000402"
     CDS             complement(42841..42945)
                     /locus_tag="SI621_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063158.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            42999..44984
                     /gene="pdeR"
                     /locus_tag="SI621_000403"
     CDS             42999..44984
                     /gene="pdeR"
                     /locus_tag="SI621_000403"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HEI4063159.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEILLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            45131..45304
                     /locus_tag="SI621_000404"
     CDS             45131..45304
                     /locus_tag="SI621_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063160.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            45394..46143
                     /locus_tag="SI621_000405"
     CDS             45394..46143
                     /locus_tag="SI621_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4063161.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            46412..46630
                     /gene="osmB"
                     /locus_tag="SI621_000406"
     CDS             46412..46630
                     /gene="osmB"
                     /locus_tag="SI621_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HEI4063162.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(46756..47082)
                     /gene="yciH"
                     /locus_tag="SI621_000407"
     CDS             complement(46756..47082)
                     /gene="yciH"
                     /locus_tag="SI621_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEI4063163.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(47082..47819)
                     /gene="pyrF"
                     /locus_tag="SI621_000408"
     CDS             complement(47082..47819)
                     /gene="pyrF"
                     /locus_tag="SI621_000408"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEI4063164.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQASAAGVDYM
                     VIGRPITQSVDPAQTLKAINASLQRSA"
     gene            complement(48011..49180)
                     /gene="lapB"
                     /locus_tag="SI621_000409"
     CDS             complement(48011..49180)
                     /gene="lapB"
                     /locus_tag="SI621_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEI4063165.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYALYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(49187..49495)
                     /gene="lapA"
                     /locus_tag="SI621_000410"
     CDS             complement(49187..49495)
                     /gene="lapA"
                     /locus_tag="SI621_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HEI4063166.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(49644..50408)
                     /gene="pgpB"
                     /locus_tag="SI621_000411"
     CDS             complement(49644..50408)
                     /gene="pgpB"
                     /locus_tag="SI621_000411"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HEI4063167.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPVEENREIAQREQES"
     gene            50578..51168
                     /gene="ribA"
                     /locus_tag="SI621_000412"
     CDS             50578..51168
                     /gene="ribA"
                     /locus_tag="SI621_000412"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEI4063168.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(51232..53907)
                     /gene="acnA"
                     /locus_tag="SI621_000413"
     CDS             complement(51232..53907)
                     /gene="acnA"
                     /locus_tag="SI621_000413"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEI4063169.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIYALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGIAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSEVISIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLIGEEKIDIVDLQSLQPGATVPVTFTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(54071..54166)
                     /locus_tag="SI621_000414"
     CDS             complement(54071..54166)
                     /locus_tag="SI621_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063170.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(54280..54447)
                     /locus_tag="SI621_000415"
     CDS             complement(54280..54447)
                     /locus_tag="SI621_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063171.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(54450..54578)
                     /locus_tag="SI621_000416"
     CDS             complement(54450..54578)
                     /locus_tag="SI621_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HEI4063172.1"
                     /translation="MPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKVWG"
     gene            complement(54909..55883)
                     /gene="cysB"
                     /locus_tag="SI621_000417"
     CDS             complement(54909..55883)
                     /gene="cysB"
                     /locus_tag="SI621_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HEI4063173.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(56093..58690)
                     /gene="topA"
                     /locus_tag="SI621_000418"
     CDS             complement(56093..58690)
                     /gene="topA"
                     /locus_tag="SI621_000418"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEI4063174.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            59070..59321
                     /locus_tag="SI621_000419"
     CDS             59070..59321
                     /locus_tag="SI621_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HEI4063175.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(59357..60406)
                     /gene="sohB"
                     /locus_tag="SI621_000420"
     CDS             complement(59357..60406)
                     /gene="sohB"
                     /locus_tag="SI621_000420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEI4063176.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNISEQYKEMKEELAAALMDTHQQKQWHKTQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            60626..61384
                     /locus_tag="SI621_000421"
     CDS             60626..61384
                     /locus_tag="SI621_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HEI4063177.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            61381..61971
                     /gene="cobO"
                     /locus_tag="SI621_000422"
     CDS             61381..61971
                     /gene="cobO"
                     /locus_tag="SI621_000422"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEI4063178.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVKALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(62023..62331)
                     /locus_tag="SI621_000423"
     CDS             complement(62023..62331)
                     /locus_tag="SI621_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063179.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFIHILTVMNNLT
                     INYGTETSPELAGINYDDANALITELILFIEDVDAENHEAYPGHLNEITRILNFAIAA
                     "
     gene            complement(62342..>62646)
                     /locus_tag="SI621_000424"
     CDS             complement(62342..>62646)
                     /locus_tag="SI621_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063180.1"
                     /translation="ASKNSKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEAL
                     NAFIDKGDYLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
BASE COUNT        15259 a        16439 c        15253 g        15695 t
ORIGIN      
        1 gtttccggca tgacgacaat tatctggagg agcctgataa aacaatgatc gctgaactgt
       61 ttacaaataa tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca ttgattctga
      121 tgagcttttg gtttcgccgc ggaaatcgta aaagaaaagg ttttttattc catgcggtgc
      181 aatttttaat ctacaccata attatcagtg ctgttggtag catcattaat tatgttatag
      241 aaaactataa actcaaattt atcaccccag gcgttatcga ttttatctgt acgtccctga
      301 ttgcggttat tctgacgatt aagctatttc tgctgattaa tcagtttgaa aaacagcaga
      361 ttaaaaaagg tcgcgatatc accagtgcgc ggattatgtc gcgtatcatc aaaatcacca
      421 ttattgtggt gcttgttctg ctttatggcg aacatttcgg catgagcctt tctggcttgt
      481 tgacctttgg tggtattggt ggtctggctg tcggtatggc cggtaaagat attctgagta
      541 acttcttttc cgggattatg ctctatttcg accgtccttt tagtattggc gactggatcc
      601 gttcaccgga cagaaatatc gaaggtacag tagcggaaat tggctggcga attaccaaaa
      661 ttacgacctt tgataatcgt ccattgtacg taccgaactc gctgttttcg tcgatcagcg
      721 tagaaaatcc tggacgaatg accaaccggc gcattaccac gaccattggt ttacgttatg
      781 aggatgcggc aaaagtgggc gttattgtcg aagccgtacg tgagatgctg aaaaatcacc
      841 cggccatcga ccagcgacaa accttactgg tttatttcaa ccagtttgcc gattcgtcat
      901 taaatattat ggtttattgc tttaccaaaa ccacggtatg ggctgaatgg cttgctgcac
      961 agcaagacgt ttatttgaag attatcgata ttgtacagtc acacggcgcg gactttgcct
     1021 tccccagcca gacgctgtat atggataaca ttacaccgcc ggaccagggt cgctaaatca
     1081 gacatcactg gcgttatcca caagggtaac gccaattccc atcacatcaa tgcttcacaa
     1141 tatacatagt ccgactgtac gccacatctt ccgggttatt aatgggataa cctttcagcc
     1201 acggcttgat taatcgtcca ttggtatatt gataaattgg tgcaatcggt gcttgctcca
     1261 tgaggatttt ttctgccgca ttgtaatcgg cattacgcgc tttaacggta ttttccgttg
     1321 atgcctgggc cagtactttg tcgtatgccg gattgttaaa gcgtgaaata ttccctgaat
     1381 gcgttgacgt taacaacgta aggaaagtgg agggttcatt ataatcccct acccatgagg
     1441 cgcggataac atcaaaattg ccggtgttac ggctatcgat ataggttttc cattcctgat
     1501 tctgcaattt aacatctacg ccaaggttct ttttccacat cgatgctaca gcaatcgcaa
     1561 ttttttgatg gttttctgaa gtgttataca aaagcgtcag cttcagcggt ttttgcggac
     1621 cataaccagc ggcgctcaac aaagtttttg cctgcgcatt cagttcttcc tgactcattt
     1681 gttcaaacgg cgaaggctcc ggcgtaaaac cggcagtaac atctggtgta aaatgccacg
     1741 ctggcttttc ccccgtcccc aacacttttt cggtcatcag gcggcgatct atcgtcatac
     1801 ttaatgccag gcgaacgcgc tgatcggccg tcggcccttt ttgcgtgtta aacgcataat
     1861 aataggtccc gagctgcggc ggcgtataaa cctgccccgg aatatccttc aacagcttct
     1921 gatacatatt tttcgggaag gattcggtga tatcaatatc ccccgcgagg taacgcttag
     1981 tggctgcgga ttcctgatta attggcagga aggtcacttt ttgcagtacc gttttggcgt
     2041 tatcccaata atgggtattc ggtaccacga ccagtttttc attgactacg cgctctttaa
     2101 gaacataagc gccattgccg atcagttttc cgggtttcgt ccactcttta ctgctttcta
     2161 cgttggcttt ttgcaccggg aagaaggcaa agttagcggt taaattcaca aaccacggca
     2221 acggtttatc aagctgaatt ttcaaagtat gggcatcaac tgcggtgacg ccaagctggt
     2281 caggcgtagc tttaccatca ataatcgcct gtgcgttgtt gattcccgcc agcgcggcaa
     2341 accatgcaaa tggcgatagc gttttcgggt ccaccagacg ctgccagctg tagacaaaat
     2401 cttgcgccgt taccggtgtg ccatccgccc attttgcgtt atcgcgcagg gtaaaagtcc
     2461 agatacggtt gtcattactt ttccactgag tcgcaacgcc ggggacaatc tcccctttct
     2521 cgttctgatt caccagtcct tcaaacagat cgcgaatgac ctgaatctct ggcaggccca
     2581 cggctttagc gggatccagc gacgcaggct catctttaat gtggcgcacc aactcctgct
     2641 tctctgccag tactgtgccg ctcggaactt ctgcagcata cgaaagagaa atgctgctga
     2701 tcaacagcgc acaacacgtg actgaaacag agtgcttcat aagtttccct ctaaccgaca
     2761 ttaaaattga tgcgtaatta tttgtttcgc cttaaagaaa tgcaaacaac ttccatcaaa
     2821 aaagtgacaa aggaagcatc aaatgtttta agtatcaata tatcaaatca gatttagtgt
     2881 aaataatagt ctccaaaatg cggtaaagca catagaactc cgaacactac gttcgggctt
     2941 cgcacgttca aacccgtttt cggtatcata atcagcatct gtcgccattg tgcgaagaat
     3001 aaaaaataac caaaacgttg aaaggaactg agaatacgtt aagagaagaa ttaaattaat
     3061 gtatcagcaa gtagtgctaa acagcacctt tatacttcag cctatctata aataatgaga
     3121 atgccgagcc agcatgtcga cggtgtggat agtacaggtg ataaccgggt aaatctggtg
     3181 tgaatttatc caaaacacgt atcagttttt tttctttaat agcccgctca acctgatcat
     3241 aaggtagata cgccaatcca tgcccatcaa ttgcagcatc aatgatcagg tctatcgtat
     3301 tcagtaaaag ctgaccttcc atgcgaacac gaacttcacg tccaccgcgt attaatctcc
     3361 agcgatttgc tgtacccgat gtgggaagat acaaattaat tgcctgatga tctattaatt
     3421 gtgacactga cgttggagca cttcgacgag aaaaataatc cggtgagcca acaatagcca
     3481 ttggtatatc tggcccgatt cgaatggcga tcatatcttt atccatctcc ccacccagac
     3541 ggacgcctgc atcaaaacgt tcagaaacga catcggtcaa accataatca atggtaagct
     3601 ggatatcaat ttcaggatgc gatttcagga atgtgcgcat agctggtaac aatatcgttt
     3661 ttgctgcatg ttctacagta gtaatacgta ttgtcccgga tgggcggttc tgcagatcgc
     3721 tcagggatgt cagggcagaa tctatgtcat gcaacatagg gccaagaaca gacaaaagat
     3781 gctcgcccgc ttcagtgggt acaacgctgc gcgtggttcg cgtcagaagc cgcaatccta
     3841 atcgttcttc tatacggcgc actatctggc ttaaagctga ttgcgccatg ctcaggcggg
     3901 ctgctgcgcg agtgaagcta cgctcctctg caacgacgac aaacgccatc agatcagcga
     3961 tttcttcacg cttcattcag tatctccata caatctatta atcatttaaa tgataggtct
     4021 aatcataatt tagggtctaa tcaacttaat gatcaatatc tatagtgata tcaccaaccg
     4081 ccaaaacgaa ccgacaactc catgaggaac ggaatgatga ataataaagt cagcttcact
     4141 aacagcaata atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat
     4201 gaaacccgtc agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa
     4261 acggccggaa cctatgccga aaaactggcg gaaaagggat ttgtcactat tgcttatgac
     4321 gcatcttatc aaggtgaaag cggcggcgag ccgcgtcagt tagaaaaccc atacattcgt
     4381 actgaagacg tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc
     4441 cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag
     4501 gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgt
     4561 aatggttggg aaaacaatgt gaaatctatt gatgccctac catacgttga cgcgggttca
     4621 aacgcccgaa ccagcgatat tagcagcgga gaatacgcca ccatgccact ggcaccaatg
     4681 aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacccct
     4741 cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt
     4801 acctatgatg cttaccatat ggcggaagtg tacctgactc agccgataca gattgtggcg
     4861 ggcagccagg cagggagcaa atgaatgagt gacgacctgt acgatcgagc atcaagccag
     4921 gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagtc
     4981 tatgtggctg aagcaatttc agtattagcg ccgttctttg aggaaactct gtaatgaaac
     5041 atattcaaat ccgtaatagt gatatggcct ggcatattgc tgccaataat ctgggaaaag
     5101 tttcatgaaa aatgtactga ttcttggtgc tggcggtcag atagcccgcc atgtgattaa
     5161 tcaactggca gataaacaaa ccattaaaca aactttattt gcccgtcagc cagcaaaaat
     5221 ccacaaaccc tatccaacta atagcaagat tattatgggg gatgtactga atcatgcagc
     5281 actgaagcag gccatgcagg gacaggatgt tgtgtatgcc aaccttacgg gggaagattt
     5341 agatattcag gcaaattcag tgatcgctgc gatgaaagcc tgtgatgtta aacgtctgat
     5401 ctttgttctt tccctcggta tttatgatga agttccagga aaatttggcg aatggaataa
     5461 tgcagttatt ggtgaacccc taaaaccatt ccgcagggct gcggatgcaa ttgaggcttc
     5521 tggactggaa tatacaattc ttcgccctgc ctggctgact gatgaagata ttattgatta
     5581 tgagcttacc tcacgcaatg aaccattcaa aggtacgatc gtatcacgta agagtgtcgc
     5641 agcattaatt acagatatca tcgataaacc tgagaaacac attggtgaga atataggcat
     5701 caatcaaccc gggactgatg gcgataagcc attcttcatg taaatcaatt atatagctac
     5761 catcgtactg tactatgtgc agtacgattt attaaactta ttaaggtgaa ctgatcaatc
     5821 agatgcgatt aacatgtgta gaaccagtaa gaataaaata cgccatgtat ataacattac
     5881 gacaattcta atatctgcac atcaatgaaa atgcaaacaa cttccggcaa aaaagtgata
     5941 caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat ttgcacaaca
     6001 ctgcctgtaa tacttcctga accagagacg atcatgaccg taactcgccc acgcgccgaa
     6061 cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg cgcgccgtta
     6121 atctggtttc ctgcccctgc agccagccgc gaaagtggtt tgattctggc tggcactcac
     6181 ggtgatgaaa actcttcgat cgtcacgctc tcctgtgctc tgcggacgtt gacaccttct
     6241 ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagtt agggttacgg
     6301 gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaattg gaaggaaggt
     6361 gaaaccgttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt gctgacaggt
     6421 gacaaacctg gctctgaacc tgaaacccag gcgctgtgcc agcttataca ccgcattcaa
     6481 cccgcctggg tggtctcatt ccacgatccg ctggcctgta ttgaagatcc cagacacagt
     6541 gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag cgtcggttat
     6601 gaaacacctg gctctttcgg tagttggtgt gcggatctca acctgcattg catcaccgca
     6661 gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc gatggcaaac
     6721 ttgctgcgct ggcatcctaa agatgcaatt cgcccgttgt gaactgaagt gccggttcca
     6781 catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc ggcaccagcg
     6841 gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc agactaaaac
     6901 cttgtgcacg tgcttcagtc gccagcgcca gcgcttccgt caggccaccc gttttatcga
     6961 gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta cgagtatgac
     7021 aactttcatc tgcacaaatc ggtaacggat gaataaaatt ctccaacgcc gcatcgtcct
     7081 gcgcaggaag cggttgttca agcatcgcaa cgcctaaatc cgccaatagc tggcaacgcg
     7141 ccgccaaccc ttctgcacgc caggattcat ttgcatcaac gatcagcgtc gcatcgggca
     7201 cagcggtgcg aattgccacc atccgctcac tgataagatg gttatccagc ttcactttca
     7261 gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga tcaggcgtac
     7321 cgatgactac cgtctgtgca gtggtgactg tctcgggaag cgttatgccg atcaaatcag
     7381 ccagcgattg ctgctgtttt cgcgccgcca gatcccacaa tgcacaatcc agcgcattac
     7441 gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct ttctctaatt
     7501 gcggcacgac gctcataatt tgcgccatta ccgaggcatc actttcgcca tagcgcggat
     7561 aaggcgtaca ttcgcccgta cctttgacgc catcttcttc cagttcaacc accaccacgc
     7621 gcgcttcact gcgacttccc cgagcaatca caaacggggt atgtaagggc cagccttcct
     7681 cgaatacctt aacggttctc atctttactc cttgttgtcg cggtggtctg cggattaagc
     7741 gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac gttaactata
     7801 tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac ccggttacag
     7861 tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc gtggcaaaag
     7921 atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg aacattttcc
     7981 cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaatcaa ctggcaaccg
     8041 agatcgacaa caccgttgtg ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt
     8101 tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt aatgctgaat
     8161 tcctgcaagc ctacggtgtg gcaattgctg atggcccact gaaaggtctg gcagcgcgtg
     8221 ccgttgtggt tattgatgaa aatgacaatg tgattttcag ccagctggtg gatgaaatca
     8281 ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataagtt aatatgcctg
     8341 atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct gactcagacc
     8401 gtattcccgc aacttattgg caatcgcggt atgcgaaacg cccaggcgtt ttgccagttt
     8461 gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa aacggctggt
     8521 gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg cggcgtcata
     8581 atccggtaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg
     8641 ataaatagcg ttctttaact gccgcacatt tcccggccac gcataacgcg taagtacagt
     8701 attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac
     8761 gaacagctcg gttaacggca tgatgtcctg cggacagtca cgtagcggcg gcagattaag
     8821 cgtcaacacg ttcagacgat aataaagatc ttcacggaac acgccttttt gcaccagttc
     8881 gacaagattc ttctgcgtcg cgcaaatcac ccgcacatcg acatgcacct catggtcttc
     8941 gccaacccga cggaaagtgc catcattaag gaaacgcagt aatttcgcct gcatccgtgg
     9001 tgacatctca cctatttcat ccaacagcac cgaaccaccg ttcgcctgct caaagaaccc
     9061 tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt ccggtataga
     9121 cgcacagttc agcgccagat agggtttgcc cgcgcgtggg cttgcctgat gacaggcata
     9181 tgcaaagaga tcttttccgg tgccggtatc accggttatc agcaatggcg cgcttagcat
     9241 cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg cgacaatttg
     9301 actgaaggcg ctgacgtcct gggcggcgac attttgcaac tgacgaccca tacgaatcgt
     9361 tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat cctgaagata
     9421 aacaggcgta atctccatca ggaaattctg tccattaata acgacatgct cgttatggga
     9481 atcctgcggt tcgctttcca gccagcgtaa aaaattaaag ccgttaatca attgcgcggc
     9541 ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc
     9601 catatccact ttgcttttca tatcgacaga gagcacaggt tcaggcaacg cttccagtaa
     9661 cgcgctcaac gccagatgct cacgttcaga aggcatccac gggacagtac ggacatcggt
     9721 aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa actccagttc
     9781 agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta aatcaatgcc
     9841 tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac
     9901 ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg atatgacact
     9961 atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga aaaacgggca
    10021 cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc tgggcgtttt
    10081 gcctttttgc agagtttctt tcacctgacc gataagctga cgacggaaat ccccgaggcg
    10141 tggcttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt taatccccag
    10201 tcgtgccgtc agaagtcctg caccaatccc ctgagctgcg cgggtagaca aacgcgcagc
    10261 gagatcttgc gacatccagt ccatccccac ttcgcgaacc agttcgctgg ctccggcgaa
    10321 agcgatattc agcaatacca gcttgaacag gcgcaaacgg ctgtaatacc ccagttcaat
    10381 gccatacagc gtggcgatgc gattaatcaa acgcagattg cgccaggcga taaatgccat
    10441 atccaccagc gccagcgggc taacggcgat cattaacgtt gattccgctg ccgaacggct
    10501 gatttcgcgc cgtgcctggg catctaaaac cggctggacc agatgagcat acaggctgac
    10561 cacttcacga tcgttctgcg tttcatggat tgaggcatac cagcgttgca gcgccggatg
    10621 cgactgatca atacccgcct gctgcgccag tttttcgcag aatgcacggc ctttacccgt
    10681 gccgtgactg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg
    10741 ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa tcaatgcccc
    10801 agcggcacat ccacccagcg ccacccagtc ctgggtttgc caggcgttca ttgtccactg
    10861 tacaccctgc ccgacaacgc ttgcgccaaa cagagccaac ccgcccatca ccattttgcg
    10921 ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt gcccctcttc
    10981 ttcctgcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt cgtcaaaggt
    11041 ctgctgcacc ctgaattttg gattctgatc gacctccagt ggaccgtcga aatcaatacg
    11101 tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag cgcagcatca
    11161 aggcgaatat gcggcagcgg tttgtcgaca tccatcacct gcggacgaaa cgcctcaaac
    11221 tggaagcctt gcttatccca gaacgcctga ccaggcaaac gtgcgggaac ttcgccagga
    11281 taaacagtga gcggtgcgcc atcactgagt cgattaccgc gcagcgccgg gattttctca
    11341 ccgttaacat caataatgcc gctggtggtc gcctgaactg acgccagccc caggcagtcc
    11401 atgctgatcc cttcgaacgc cgcattttgc caggcatcct gaattagttg ttgcagtaat
    11461 gaaaccatat tggcgtgctg atcgatggtc acatggtctg ctttagtcgc agcaaacaat
    11521 agcttatcga taaccggcga aaacaaacgc cggaacaggg ttcgctgccc gtagtggaaa
    11581 ctttgcatca gctgcgtcag cgccagacgc atatcattaa atgcctgtgg cccactgttg
    11641 agaggctgca ggcaatccac cagcacaatc tggcggtcaa agcgcagaaa atgattctta
    11701 tagaacccct tcaccacctt ctcgcaataa taattaaagc gctcgcgcag cattccggca
    11761 ttggtatgct tatcggcctg cgccagtttg gactcgcccc aggcatcgac atccggccac
    11821 gggaagaatt gcagcgcggg tgctcctgcc atatctcccg gcaagacaaa gcgcccaggc
    11881 tgaataaagt gcaacccctg ctgtttgcag tggtggagat actcggtcca ggcggcggca
    11941 atgtctgcca gccggttttc gtcggcaggc gctagcgggt ccagcccttc gcacatcatt
    12001 cgccatttcg ccgaccattc gccgcgctga ccattgagta agcccgtcat ctggcgcgac
    12061 cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc ggggtaatcc
    12121 acaatttcca gatacagcgt ggaggtgtcc ttaaagtggc gtagcagcga atcgttcgat
    12181 ttaaaacgta acgccaggcg aatttcactg acgccgcgcg taggcgtcgg ccaggcggga
    12241 ggatcgccat ataactgcgc caacccttca tcataggtga agcgcggaat gccaaagtca
    12301 cgctggggaa tgcgttttac gcccagcagg cgctcttcac gcaccgcact taaaagcggc
    12361 aaacgtgctc cggcatgaat attgagcaac tgattaacca tcgcggtgat aaacgctgtt
    12421 ttgccgctgc ggctaagtcc ggttacagcg aggcgcagat gtctgtcgac accccgattc
    12481 accagcgcat taagttcatt tttaagtcgc ttcattgctg tcctgtgcca gaaagctgta
    12541 gaaaggatta gatactggag ataataatgt gggttaattt tgatttatca aatgagtaag
    12601 cggcgttgtt tacaccgctc aataagtaat ttgttaacct ttgaccttcg gcattgcgat
    12661 gtctttcagg ccgccggcgt tctcaacgtg ggtatatccc atctcgctaa ggatctcttt
    12721 tgcctgccct gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
    12781 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ctccctgaac
    12841 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
    12901 gggcagtgaa aacaccagtg tcagagcaag taagcctttt ttaaacatgt aagcgtcctt
    12961 ctatggatga aaattgccat ccataatatg ccttagcttt aacaattttt gcgaaactcc
    13021 cctcaccttt tataacgctg cgccagttta ttggcagccc gacttaacag cggttccagc
    13081 gccacggcca gcaacatttt cagtggtcgg cgagcaactg attttatcgc ccagccagct
    13141 acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa
    13201 ccaggcttca ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt
    13261 gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta
    13321 aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattca ctgctggaag
    13381 gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga
    13441 tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac
    13501 gcaccagttt tactggtaca tcaaaatagt tggcaatccc ggcgcagacg ccacggacca
    13561 tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc
    13621 cctccagttc ggatgttctg catcaagaat ggattccagc gcctgaatac gttcgcgcat
    13681 ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact
    13741 gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa
    13801 aatggttaac ggaatagcca gaaatagcgc gctcataagg actccttaga cgatgagcgg
    13861 cggcgcgttc agacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
    13921 aattgtgcca gttgctcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc
    13981 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaattcga
    14041 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccattgtc cagctgacga
    14101 cgcacatcgc gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg ctggcgagcg
    14161 cgtgtttcgc tcaatttgtt ttccagctcg ccgatctctt tcttcatgcg tgccagcgta
    14221 tcgtccacca gcgtcacttc atgttccagg ctcttaatca gatcggtcag tttctgtttt
    14281 tcaattaacg ccgcacgcgc cagatcctct ctctctttca gcagcgccag ttcggctttt
    14341 tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt
    14401 tctgccagcg cacgcgccga agtagaacgc acttcaacca atgtatcttc catctcctgg
    14461 atcatcaaac gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg
    14521 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg
    14581 cgttcaggat tgtcctgctg aactattaat acaatttgcg tgccaatttt ttatcttttt
    14641 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgagt tagcgaatta
    14701 cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact cggtgaggcg
    14761 aacagctttc tcgaggtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg
    14821 ctcatcatcg gcgagcgcgg cacaggtaaa gagctgattg ccagccgtct gcattatctc
    14881 tcctcccgtt ggcaggggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
    14941 ctggattccg aactgtttgg tcacgaagcg ggcgcgttta ccggtgcgca aaaacgtcat
    15001 ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca
    15061 ccgatgatgg tgcaggagaa attattgcgc gtgattgagt acggtgaact ggagcgcgtt
    15121 ggcggcagcc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc
    15181 ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc tcgaccggct ggctttcgat
    15241 gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat ggcagaacac
    15301 tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc
    15361 gccagagaaa cattgctgaa ttatcgctgg ccgggaaata ttcgtgaatt gaaaaacgtg
    15421 gtggaacgtt cagtatatcg ccacggcacc agcgattatc cgcttgatga cattattatt
    15481 gatcccttta aacggcgtcc gcctgaagac gctatcgccg tttcagaaac cacctcgctt
    15541 ccaacactgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga gttgctgcaa
    15601 ctcagtttgc aacaagggaa atataaccag aagcgcgcag ctgaattgct ggggttaacc
    15661 tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg ggcaatacaa
    15721 cttgccggat gcggcgtgaa tgctttgtcc ggcatacata atcaatacgt cgtttgcagg
    15781 cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgtt cagcgcatcc
    15841 tgcaatattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaaa
    15901 cgaatcacgt tgccgtatgc gccacaggtc agcagaagca gcccctgcgc cagcgcgcgt
    15961 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
    16021 gccgcaatca tagaccccag accgcgtacc gccgcgatgg acggaacgct ttctttggca
    16081 tcaatcagcg tgtttgtcag acactggccc agttgattcg cgcgttcgca gagtgattct
    16141 ttgtcgataa tgttgagcac cgcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag
    16201 gtaccaccaa gcccgcccgg cgcgggtgcg tccataatgt tggcattacc gaccacgccc
    16261 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaaatccgg cttatcggcg
    16321 tagtgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac ttcatcggca
    16381 atcatcacaa tgccgtgatc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggt
    16441 gcaacgttga aaccgccttc gccctgcacc ggttcgaaaa taatcgccgc cacctgcttc
    16501 gcttcaatgt ccgatttaaa caagcgttcg atggcgtcga gggagtcctg cgttgaaatg
    16561 ccgtgtaaat ctgacggata aggtacgtga tataccgaac cggggaacgg gccgaagccg
    16621 attttgtacg gcgcgacctt tccggtcagc gccatagtca tatatgtacg accgtggaag
    16681 ccgccgctaa acgcaatcac gccagggcgt ccggtatggg cgcgggcgat tttcaccgcg
    16741 ttctcaaccg cttccgcacc ggtggtgaag aacgcagttt tagcctgccc gctcaccggg
    16801 gcaagggcgt taattttctc cgccagcgtg acgtagcttt cgtacggcac aatctgatac
    16861 gccgtgtggg taaactgttg caattgctgc tccaccgccg cgaccagatc agggtggcga
    16921 tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tatattcgtt gccctctaca
    16981 tccttcagcg tagcgttttc tgccgactgg gcgaagaagt tacacatcac gccaaccccg
    17041 cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
    17101 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc
    17161 cgcgcccatc gcggtaaacg gaacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg
    17221 cagattggcg aaggcgtcga agcgttcggc gtcaccgcgc agcagttcag caatcaaacg
    17281 cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt
    17341 ggtatcaagg cgaccgaact gcggcatgcg cgacaaggtc agcaggaagt tgcccgtcca
    17401 gcggtaatca attttcacgc ccttcagctg cgggaaggtt ttcagcagtt tcggcaccac
    17461 aaggcgctca acgtcatccg ggtcgcgcgc gccgtagacc acaccgccgc cgtacagcag
    17521 gcggttatcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
    17581 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcagtgg tgatcacctg
    17641 cgtgccgcac ggcatgctgc gtttcgccag ttccggctct actttatcgc ccagatacgc
    17701 attcccggcg acaatcacat acttcgccgt cacctgacca ttggcagtcc gcaccacggc
    17761 tggcgtggtg tgctggatct gcgtcacggc ggaaagctca tacacgcgcc ctccgttcag
    17821 gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgtc caccgctgtg
    17881 atccagaagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc
    17941 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttccagagt
    18001 cgcgagctgt ttatcattca tcgccacaaa caggccgccg gggcggtagt cgcaatcaat
    18061 ctgataacgt ttgatacgtt cgcggatgat ctcgccgcct tcgaacatca tgctgccgag
    18121 catacgggcg gtgtccatgc cgtagctttt ttcaatgacg tcgatgtcgc ggctgtagga
    18181 gttcacaagc tgcccgccgt tgcgtccgct tgcgccgaag ccgatgcgtg aggcttcaag
    18241 aaccaccacg tcaaagcccg cttccgccag atgcagcgcg gaggaaagcc cggtatagcc
    18301 gccgccgacc acgcaaacat cgcaggaaat cgactcattc agcgtgtcga atggtgcgta
    18361 tttattcgca ctggcggcgt aataactgct ggtatgttcg gtcatttcag gcctccaggc
    18421 ttatccagat agttttcagt tcggtgaatt tttcaagggc gtgcagggat ttgtcgcgac
    18481 cattgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
    18541 tgacaaagac ggaaccggct ttcaggcgac ggctcatgcg gtgcgcgcgg gagaggtcgc
    18601 gcgtccatac tgccgcgcca agtccgtact ggctgtcgtt agcaagctgt agcgcctgtt
    18661 cttctgatgt gaaacgcgtg accaccagca ctggaccgaa aatctcttcg cggcttaagg
    18721 acgcattcgg gtctacatcc acaaagatcg tcgggccgat ggcggcagcc agctcggcgt
    18781 tacggccatc cagcagcagt tgccctttgc tttcgccttc acgaataaag ctatggaccg
    18841 agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc
    18901 ccggctgcca gttttgcgcc tgctgtttta acaggtctaa gaattcatcg gcgatgctct
    18961 cttccagcaa caggcgcgtt ccggcgatac acacctgccc ctggttgtag aagatgcctg
    19021 ctgcggtcgc gctcgccgct ttttgcaaat ccggacagtc agcgaaaacg atgttggcgc
    19081 ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct gtcgcctgca tctttcagca
    19141 gctgtttccc ggtacgggtc gaaccggtaa aggcgatggc atcgatatcg ttatgacgcg
    19201 acagcgcctg ccccgcttca tgaccaaaac ccgtcaccac gttcaacacg ccatccggca
    19261 gacctgcttc tttcgccagc ccggcgagac gaatcgcact gagcggtgat ttttcagacg
    19321 gttttaggat cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca
    19381 gcagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca
    19441 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atagcctcgg
    19501 tgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcaggctg tgacgaatcg
    19561 gtttgccggt gtcgagcgtt tccagcagcg ccagctcttc ggcgttggct tccataaaat
    19621 cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
    19681 gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
    19741 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaagtt tcattttccg
    19801 ccgcagcagt atattcaccg ttaataaata agcgggtttc aatggcgaga cttaacgctt
    19861 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
    19921 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc ccgtttattt
    19981 ctggcgacat tctttataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    20041 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
    20101 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
    20161 atcctgacca ttaatcgtca gaacaatttc accttccagt acagtgccta tttcctcacc
    20221 ctgatgctta attctttccc cagtggttgt gcccggctgg taagtttcaa agatcatcgc
    20281 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
    20341 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    20401 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    20461 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    20521 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    20581 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    20641 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
    20701 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    20761 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    20821 ctaacgacct tcgccccttg tccatgcaga gagtttaccc aaaagttgct acattcaggt
    20881 aacaacgcag acagtaatcc cccctcttca accagaactt catgcgacgg cgcatattgc
    20941 tgctccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    21001 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    21061 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    21121 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    21181 ggactaccag gaagataaat accatcgagt ttcggcaaaa gttgttcaag taatgacggt
    21241 tccgccagcg catgtggcag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    21301 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    21361 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
    21421 tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    21481 ttttcatttt tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg
    21541 aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    21601 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    21661 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacacaatat
    21721 gttgatgttt tacttaccga tttgaatggt tgcttccgtg gaaaacgtat tcctgtttca
    21781 agccttaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt
    21841 ctgggtaatg tagtagaaga agcgggcctg ggtcaggaaa tgggcgagcc ggatcgtacc
    21901 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tataggtcag
    21961 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    22021 cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggtcgt agcggtagag
    22081 ctggagttct atttactgga tcgccagcgc gacgctgaag ggtatctgca accgccctgc
    22141 gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    22201 cacttcgccg acgtgctcaa tgatattgat gagctggcgc agttacagct gattccggca
    22261 gatggcgcgg tcgccgaggc ctcgccaggt cagtttgaaa tcaatcttta ccataccgat
    22321 aacgtgctgg aagcttgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca
    22381 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    22441 gggatgcata tccatatcag tatgcaaaat aaccgtggcg agaacgtgct ttctgacgcg
    22501 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
    22561 tcatcaatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
    22621 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccc
    22681 tgcggcgatc gccataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaacccg
    22741 tatctggtga tggcggcgat ttttgccggt attttgcatg gccttgataa cgaactgcca
    22801 ttacaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
    22861 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    22921 cgcttctgcc atgtctatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    22981 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aatacccctt gcggggttgt
    23041 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    23101 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    23161 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
    23221 tcctgcgact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
    23281 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aactatggct attaattcac
    23341 cactgactat tgctgcgcaa tctggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    23401 aggtggtgat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
    23461 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
    23521 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    23581 cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat
    23641 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
    23701 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
    23761 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
    23821 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
    23881 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
    23941 cgattattac cggggcgacg attgtctgtt tctccttcct cggtttcgat gcggtgacca
    24001 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    24061 cggtatacgg cggcgttatc tttatcgctg cgtcgttctt tatgcagctg ttctttcccg
    24121 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgcta tacgtcggcg
    24181 gcaagctctt ccagtcgatt ttcctctgta ccacgtttgt gaacacgtta gcgtctggcc
    24241 tggcgtcgca cgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
    24301 cggagcgcgt gtttggctat gtgcacccga aatggcggac tccggcactg aacgtcatta
    24361 tggtcgggat tgtcgcgttg tcagcgctgt tctttgattt agtcaccgcg acagcattga
    24421 ttaacttcgg tgctctggtg gcatttacct tcgtaaatct gtcggtgttt aatcatttct
    24481 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
    24541 tggttggcgc gctgacggtg ggtgtgctgt gggttaatct cgagtcaacg tcactgacac
    24601 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    24661 gcaaagtgcc gttgtatgat ggtgacagag cgccggtgag cgaaacgtaa tagtcggatg
    24721 cgcggcgcat tatcctgcct gagggtaatg caccgcgcca ttaactataa aaagatgtat
    24781 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccagggggaa
    24841 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    24901 tgcgcagagc ctgtcgcaga cgctgaacgc acaccgctgg ccctctactt ccagttactg
    24961 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    25021 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    25081 aatgagtaat tatttcattg cagacgctcg gtcagtatcg ttgcccggat aaagcgttca
    25141 cgccgcatcc ggctaacaat gacgcgcact gcagcgtgct ttaatgggtt tctcacgatc
    25201 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    25261 acgaatgttg aacagatggc gacgtcacct gttcaacgac caaccacacg gccctcatta
    25321 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
    25381 tacataaaat ccattaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    25441 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgat
    25501 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    25561 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
    25621 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagttct cgacaacggc
    25681 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    25741 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    25801 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    25861 gccgataacg tgaaaagcgt ccgcaaactg gataatcata cggttgagtt ccgactcgcc
    25921 cagccggatg cttctttttt gtggcacctg gcgacccatt atgcatcggt catgtcggca
    25981 gaatatgccc ggaagttaga gaaagaagat cgtcaggaac aactcgaccg tcagccggtc
    26041 ggcactggac cgtatcagtt gtcggaatac cgcgccgggc aatatattcg cttacaacgt
    26101 catgatgatt tctggcgtgg taagccgttg atgccacaag tggtggtgga tttaggctct
    26161 ggcggtaccg gacgtctgtc gaaactcctg accggggagt gcgacgttct ggcctggcct
    26221 gctgccagtc agctatccat tttgcgtgac gacccacgct tgcgtttaac gctgcgtcct
    26281 gggatgaacg tcgcctatct ggcattcaac accgccaaac cgccgctaaa taatcccgct
    26341 gtccgccatg cgctggcact ggcgattaat aaccagcgtc tgatgcaatc catctattat
    26401 ggtacggctg aaacggcagc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    26461 gctaaaatta ctgaatataa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    26521 gaaaacttaa cgctgaaact gtgggtacca acacgttcgc aggcgtggaa ccccagtcca
    26581 ctgaaaactg ccgaattgat tcaggcggat atggcacagg ttggcgtaaa agtggtgatt
    26641 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    26701 ttatccggtt gggcgactga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    26761 tgcgcggcaa ttcattcgca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    26821 gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa
    26881 gcgcagagta ttctggcgca ggaattaccc attctgccgc tggcgtcgtc attgcgcttg
    26941 caggcctatc ggtatgatat caaaggtctg gtacttagtc cgtttggtaa cgcctccttt
    27001 gctggggtgt atcgcgagaa accggatgag gtgaaaaaac catgattatc ttcaccttac
    27061 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    27121 gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    27181 ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg
    27241 ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt
    27301 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    27361 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    27421 cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    27481 tcgatctgct ctacgaagtg aaaccgatta ccgattttgc gttgattgat gcctggcttt
    27541 cggactcacc atggcgggat gaaatggtca tgagcgcaat ccgccatatg atattgcccg
    27601 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg actgatgcgt atcagcacta
    27661 tcgaagtgta tgaccagaac tatgttaaag cagcggcgac ccgcggtttg tcgcgcttta
    27721 ctatcttgcg tcgccacgtt ctgcataacg cgttacctcc ggttattcct cgcctgggct
    27781 tacagttttc taccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    27841 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    27901 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    27961 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    28021 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    28081 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    28141 gcgccttacg gcatcgatca gcaatttctc ggttatcaat tattgccgcc gtcatggtcg
    28201 cgctatggcg aagtttcttt cttccttggg actgacgacc tgggacgcga tgttttaagc
    28261 cgtttactga gcggagctgc gccaaccgta ggcggcgcat ttgtggtgac gcttgccgcg
    28321 acgatctgcg gcctgatact gggcaccttc gccggcgcga cgcacggcct acgctcagcg
    28381 gtgcttaacc atattctgga taccttactg gcgatccctt ccctactgct ggcaattatc
    28441 gttgtagcgt ttgccggacc gagtttgtct cacgccatgt ttgccgtctg gctggcgctg
    28501 ctgcctcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    28561 gttatcgctg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    28621 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    28681 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    28741 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    28801 ggtgcggcaa ttatgattag cgtgctgttg gttaacctcc ttggcgacgg tgtccgtcgt
    28861 gcaattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    28921 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgcgt aagtatgacc ttaaccgaag
    28981 gtgaaatccg cggtcttgtt ggcgaatccg gttccggcaa aagtttgatt gcgaaagcaa
    29041 tttgtggggt gaataaagat aactggcgtg ttacagctga tcgtatgcgt tttgatgata
    29101 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaagct ggttggtcac aacgtgtcga
    29161 tgattttcca ggaaccgcag tcgtgtcttg atccttcaga acgagtgggt cgccagttga
    29221 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    29281 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    29341 gcagttttcc ctatgagttg accgaaggcg aatgtcagaa agtgatgata gccattgcac
    29401 tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa
    29461 cccaggcgca aatctttcgc ctcctgacgc gtctcaacca aaacagcaat accactattt
    29521 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    29581 actgcggtca aactgtggaa accgcgccaa gtaaggagtt ggtgacgatg ccacatcatc
    29641 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    29701 gtcgcctcaa tacgctgccc ggcgctatcc cactgctgga acagttaccg attgggtgtc
    29761 gtctggggcc gcgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    29821 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    29881 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg
    29941 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    30001 acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg
    30061 ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct gcattttggc
    30121 gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    30181 aatccccgtc aacgtatctc gcaaatcctg gattttccac tgcgtctgaa caccgacctg
    30241 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    30301 gatcacgtca gttactatcc gcatatgttg gcacccgggc aaaaacagcg tctggggctg
    30361 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcctcactg
    30421 gatatgtcga tgcgttcgca gttgattaat ctgatgcttg agttacagga aaaacagggc
    30481 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    30541 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgccgatgt gctggcatcg
    30601 ccgctgcatg aactcaccaa acggctgatc gccggtcatt ttggcgaggc attgacggcg
    30661 gatgcgtggc gtaaggaccg ttagtgttct gcacctcatc ctgacagggc ccccctcagg
    30721 atgaggtgtt cattactttt gattgtcgat tctggtgaga gcaaccagca atgccagcgt
    30781 ataacacacc gtaattgcca gactcatttt taacagtgtt tctccgccaa accccgacaa
    30841 tggcgcggct ataccgccga agacaaacat gctcatcccc atcaacgcag acgccgttcc
    30901 agattcctgc gtccctactc cactcatagc ctctgccccg ccaacagtgc cgatgccgct
    30961 acaaaatgca atagtgaaaa ataatgccac cagtgccgga atgggcaatt gtgtccatgc
    31021 gcataagacc gtgagcaatg cacacaaaat tgccgctatc aggccacctc gcaacaatgt
    31081 catcgcgtta atccgtcgcg ccaggcgcga gaagatccat gaagcaatga taagtccgat
    31141 gccgttaagg ccaaacacca ggctaaattg cattggacta aagccaaatt ccttctgcaa
    31201 cacgaacgac gaagagccga tatatgcaaa caaaccggcc agcataaaac tttgaatcag
    31261 gcaaaagcgc ataaaacggc ggttctgcac cacgcttcgt ccggttaata gcaatgatga
    31321 gcccctttta ttttctggca aggtctcatt aataaataac aggcagccca gcaacagtac
    31381 ggtgctaatt tcagccatta cccagaataa agtgcgccag tcaaaagtgc tgacaatgta
    31441 cccgcccagc actggcgaca acaccggtgc caggccattc accgtcatta acagcgcaaa
    31501 aaactgggtc aacgttactc cctgatattt gtcacgagca atagaacgag agagcaccga
    31561 accacccgcc cccgcgatcc cttgaataaa acgccagacc accagccagt aaatattgtt
    31621 cgttgtcgcg cacaaaatgg aagaaacaat aaacaatagc aacgacaaga ttaacggacg
    31681 tttacgccct attttgtcgc tcagagggcc aaataacagt tgtccgacgc caagaccgat
    31741 cagcgatgcg gtcagggtta attgcgttat ggtcgtggtt gccgccagtt gctcagacat
    31801 ctccggcaaa gccggcaaat aaagatcggt acacatcggg ccgataccgg ctaaaagacc
    31861 aagaatcaat gcccatgaaa gagagactct agccatatta ttccttactt gagtgatgac
    31921 atgccgccgc ccagcgactg ccagagcgtg attcggtttg taaaatcagt cagttgtagt
    31981 gccagcagcg cctgctgtgc gctccacaag ctgcgctgcg ccgtcaacac ggtcaggtaa
    32041 tcaccaacac ccgcctgata gcgacgcaag ccaacatcga ccgtttgttg ctcagcttta
    32101 acatactggc gctgtgcatc cagttgctct tcaagcgtgg tccgccgcgc cagtgcgtta
    32161 gcgacatctt taaatgcgct ctgaacggtt ttttcatagg tggcaatgag tccacgcttt
    32221 tgtgcttctg cgtaacgaag ctgcgccaga ttacttccgc cagtgaacag cggtaatgtg
    32281 accgatggcg taaatgacca gatctgcatt ccgtggctga acaacgacga caatgcttca
    32341 ctgccaacac cagcgctggc agtcagcgaa atcgtcggaa aaaagttagc ccgcgccgcg
    32401 ccaatatcgg cattggcgct cttcaaatta tgttccgctt cctggatatc cgggcgacgc
    32461 agcagaacgt cagatgacac tcccgcagga accagactta tcatctgttc cggcaggctt
    32521 tccagtgttc ccggtagcaa attttcctca agcgttgtac ctgccagtaa gttcagcgca
    32581 tttttatcct gcatcacgtg tgtctgatag ctggcaacgc tggcgcgcgc ttgctgataa
    32641 acactcatcg cctcactgac atctgtcgct gccgccgtgc caacctgttg ctggcgctgg
    32701 ataattttca atgagttttc tgcactggcc atcgtttctt ttgccagggc caggttgctg
    32761 ttatcagccg ccagtgtcag ccaggcggtg ctgatttcag caatcagcgt taaacgagtg
    32821 ttctgggctg taaattcact ggcaagccag gtttcccgcg ccgcccgaga taaactctga
    32881 ttacgaccaa acagatctaa cgtaaaactg gacaccgtgc cgtcagcctc ggcagtggtt
    32941 tccgtaccat tcgccagcga acgactgcgg gttgaactta aggccgcatt caccgtcggg
    33001 aataacgacg cattagtttg cccatagagc gcccgtgctg agtcaatatc agcaatcgct
    33061 ttttgcacat cgcggttact gttcagtgca atagtcacca cctgctgcaa tcggggatcg
    33121 tggatcacct gctgccagtc atggctgatt gcctttccct ggccctgtgc tcccggcaag
    33181 gtcgccggga ttggggattc aggcgtgctg tagtggggct cgagcgagac acatcccgca
    33241 ctcaacagca ccagaaaaat aagagaacga cgcaacataa cttactcctg acggcgcggt
    33301 ttaccggcaa acaaacgttt caccagtaca aaaaacagag gaacaaagaa aatagccagc
    33361 aacgtagcgg tcagcgtacc gccaataatg cccgtaccaa tggcgatgcg gctgttcgcc
    33421 cctgcgccgg ttgctatcgc cagcggcatt acccccgcaa taaacgccag cgaggtcatg
    33481 atgattgggc gtaaacgagt ctgcgccgcg cgtaatgccg cacgactcaa ggaatagccc
    33541 tctgcaaccg ccgcttcagc aaattccaca atcagaatgg cgtttttcga cgacaagccg
    33601 atagtggtta atagcgccac ctggaagtaa acatcgttgt ttaaatcacg catccaggcc
    33661 gccagcgccg cgccaagaag ccccagaggg atcaccagaa tgaccgagaa cgggactgac
    33721 cagctttcgt acagtgctgc aaggcagagg aaaaccacca gaatagaaac tgcatacaga
    33781 ctcaatgcct gaccgctggc cagtttctcc tgcaatgaca aaccactcca ggcccaggtt
    33841 gttcccgcag gaagactgtt tgccagttcc tccatttttg tcattgcatc gccggaacta
    33901 aagccagtcg cattttcccc ctgaatttca taggctgcag aaccgttgta gcgcaccagt
    33961 ctttccggtc catacagcca gcgggtggtt gcaaaagcag agaatggtgt catggcgtta
    34021 tcgctaccgc gcacaaacca tttacctaag tctgacggcg tggaacgaaa ttcactgtcg
    34081 ccctgaatgt agactttttt cacacgacca cggtcaataa agtcattcac ataagtaccg
    34141 ccccacgcac tggacagggt gtcggtgaca tcattcaaac tcaaccctaa tgacaccgct
    34201 ttattactat caatatctac ctgcaattgc ggcatttgtg gcaaatcatt ggcacgcaca
    34261 gaatgtaact ccggactttg attcgctttt tctatcaact gattacgcat ttgcagtagt
    34321 gtttcgcgat cggttccacc gttagctaac aattcaaacg taaaaccatt gctttgcccc
    34381 agcccatcaa cggctggcgg cgtcatcgca aataccgtgg catcacgaat tgtgcccagc
    34441 tctttggttg cccgtagggc gatagcctgg gcggtgtttt ctgccccttt acgttgagac
    34501 cagtttttca acgaaacaaa cgccatcccg gtgttctgtc cgctgccgct aaaactgaaa
    34561 ccatcaacgg taaaaatgac atcggtattt gctttctcat taatcaggaa ccagtcaaca
    34621 atctggcgat tcacttctgc tgtacgggca gcggtcgccc cggccggcag cgtgtactgc
    34681 accatgattt cgccctggtc ttcagtgggt aagaaactgc ccggcagttt ccacatcatc
    34741 agggccattc caccacccag tacgacataa agccccatcg ttcgagcagc acggcgcagg
    34801 acataaatta cgcctcgctg atatttatct tcagtacggc ggtaaaagcg gttaaatgcg
    34861 ccgaaaaagc cttttttatg tggcggaaca tgctggagga cggaaccgca cagggcggga
    34921 gtcaaggtca atgccactac cacggaaagc agcattgcgg agataatggt tatcgaaaac
    34981 tgacgataaa ttacccccgt agatccccca aagaaggcca taggcaggaa tacggctgac
    35041 aacaccagcg caatggcaac cagtgcgcca gaaatctcgc ccattgattt ttccgtggct
    35101 tcacgcgcgg gcaacccttc gtcacgcata atacgttcga cgttctccac caccacaatg
    35161 gcatcgtcca ccagcaagcc gatcgccagc accattgcaa acaacgtcag ggtattaatc
    35221 gaatagccaa acaacgcaag aacgccgaat gtgcccagca aaacgacggg gacagctaac
    35281 gccggaatca gtgtggcacg caggttttgc aggaataaat acatcacgca aaccaccaat
    35341 acgatggctt caaacagcgt ctgaattacg tcctctacag agattttaat aaattcagtg
    35401 ctgtctttcg ggtatgcgat gtcgtatccc tgcggcatgt tccgctggaa ttcggcaatc
    35461 ttatccttga ccagcgtcgc cgtattcagc gcattcgcac cgggcgaaag cattacagca
    35521 accccggcag acgggtgacc gttaagtttg ccgatagcgg tataatcttc actgcccatc
    35581 tcaacgcgag ccacatcttt tatacgaaca actgcgccgt ctgactggct tttcacgata
    35641 atatttttga actgatccac cgtctgcaag cgagactgcg cacgtaccgt tgcggtcagt
    35701 tgctgagtat tcggtgaagg caatgcccct attttcccgg cagtaacctg cacgttttgc
    35761 gcttcaatag cactttgcac gtctgaaggc attagcgagt aagacgccag tttggccggg
    35821 tcaagccaga tgcgcatagc gtattccgcg ccaaagactt gcagactccc cacaccttca
    35881 acacgcgcca gcggatcctg aacgttacta accaaccagt cggcgatatc cgaactggag
    35941 gctttgtcag tggtgtcata cacggcagca atcagcaaaa agttgctttg tgatttctcc
    36001 accgtaacac ccgtttgctg cacttcgctg ggtagacgcg actccgcctg ctgaatttta
    36061 ttctgcacct gaacctgtgc agtatctgga tcggtacctt gttcaaaggt cacattaata
    36121 ctgaccgaac catcagagct gctggttgag ctgaaataga gtaaattatc aagcccagtg
    36181 agttgttgct cgataacctg agtcacgctg ttctccagcg tttcggcaga agcaccagta
    36241 taagtggctg aaattttaat ggtcggtggc gcaacgtcag gatattgcgc gacgggcaat
    36301 gtgcgaatag ccagaatccc cgccaacata atcaaaatgg cgataaccca ggcaaagacc
    36361 gggcggcgaa caaaaaagcg cgaaaacatc aggcgttgcc tccattagcc tgaacttcaa
    36421 cagccttgac ggtctgccct gaagtgactt tggcagaacc ttcaacaatc agtcggtcgc
    36481 cgttgtgcag gccgttcagc accagccatt tatcaccata cgtttctccc gtttcgagcg
    36541 ttcgctgctc tactttattg tctttattca ccaccagcgc agttgcattg cctttagcat
    36601 cgcgcgtgac gccctgttgc ggcgcgagaa tagcgtcttc catcacgcct tcatcgacac
    36661 gagcgcgaac aaacattccc ggtaataact gctgttgtgg attggggaaa atcgcccgta
    36721 atgtcaccga accggtagac tcatctacag cgacttcggt gagttccaga cgcccttttt
    36781 cgctgtaggt tgtgccatct tccagaataa gtgagacgct catggtgtca ctgttggtcg
    36841 ccagtgactg tttacgcaga cgtaataaat cgacactgga gcgagtgagg tcgacataca
    36901 ttgtatccag accacgaaca gtcgttaacg ctgtatcttg cgacgcggtc accagtgcgc
    36961 caggggttac cgacgaaatg ccaatgcgcc ctgaaatcgg tgcggttacc gtggtccagt
    37021 caagattaat gcgcgcagtt tccagtgcgg cttttttcgc ctctacactg gctttatctt
    37081 gtgcacaggt agactgagca tcatcagcat cctgttgtga aacaccgttc tctttcacca
    37141 gtcgggcata acgctgcgct ttctggcaat cggcttttac cagtgcctgt gcttgttgta
    37201 atgctgcccg agcttcattc catgcagcct gataactggc cgcatcaatc tggtagagcg
    37261 gctgtccagc cttgaccaga tcaccttcct taaataagcg tttctggata attcccccaa
    37321 cctgcggacg tacttcagca ctgagcgcag cactggtgcg tccggttaat tcactgacta
    37381 ccgaaaccgg ttgagatttg actgtaacaa cgccgacttc tgtttctgac ggggatgaat
    37441 tgttactttg cgtattatca caacccgata agagcagcat tgccactaca gatgttgcta
    37501 tatacttcat tattttctcc agggtgagaa ataccctcct cattaaaatt aatgaggata
    37561 tgtcagggat attcagttat aacgaacccg catcgcgaaa tgaatttcgc aatgaaataa
    37621 gggggattaa tatcccccgc tattggttaa ttctgttctt atttatttat caacatacta
    37681 acaatatcct gatatatttc ctgcagatgt tgtggatcgc ttttttgcgg tgttaaacga
    37741 cgataaaccg taccttccat catggtagca aacacttcaa cgcagcaacg aatgtgctcg
    37801 tcgctaagat ggggatgcat tcttttcata tgtgcacaac cgttagcgaa cattctggcg
    37861 tcagcttctt caagcattgc cataacccgc gggttgcgtg tggcttctgc agccacttcc
    37921 agcatcagcg cgtcgtcgtc ctcatttaag gatttacgca aggcaaggac ttccgggaag
    37981 tgatcggtac gcgcgtcaat gtccatttgg gcaatacgaa agtcgatgat acggcgaacc
    38041 atctcttcaa taatggcatc tttgttggcg aagtaacgat atatctgacc cacgctaagc
    38101 tgagcttcgc tggcaatttg cgacatactg gcggcatgaa acccgcagcg acgaaaacac
    38161 cgacgggcgg cgttaatgat ttcatcctga cgctgtttgt ggcgtatctc cagtttgctg
    38221 gtcatttctg tctcctggcc tatcacttgg tgagaatgat cgttctcatt tttgctgatt
    38281 atacgtttct ccaaagtaat ggaaagtatt tttatcttat tcatggtgca tattctgata
    38341 atgaagagat tgtggagatt ctgtgaagat agttgacaat attaagttgg ggtcaattca
    38401 tcagaaaggc tgctacttgt tgtcgccagt tctgttttca tcatcaaact tatcggagct
    38461 aatacgaata gtttcagagt cagattctat aagatattcc cccaacctac cccttgcagg
    38521 cgtcaagagt agataacgca aaatgctctg tacctcagct tctctccggt acagaaagcg
    38581 caactatagc cacccacagc caggttgatt ataataaccg tttatctgtt cgtactgttt
    38641 actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag
    38701 ctatgggttt tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca
    38761 tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc
    38821 agaacgacaa actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg
    38881 ttctgcagtg cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactggcga
    38941 aagtttggcc gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcaac
    39001 tggatggtga ctatgttaat gccgttaccc gtgaaggctt caaaattgcc cacgacatca
    39061 gctcctacag cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg
    39121 ccctgctgac cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg
    39181 gtctggcaaa agcgtctctg gaagcaaacg tgcgctatat ggcgaacgcg atgggtccgg
    39241 aaggtgtgcg tgttaacgcc atctctgcgg gtccgatccg tactctggcg gcttccggta
    39301 tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg
    39361 ttaccattga agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta
    39421 tctccggtga agtggtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg
    39481 aactgaaata atcgttctgt tggtcaagac gggcggcgtt ctgccgcccg ttatctctgt
    39541 tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca
    39601 ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa
    39661 aagccactgg tatcatgaaa cgcaatccag tactgcggaa tatgacgttc tgcctctggt
    39721 cccggaagcc gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac
    39781 gctggccccc ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc
    39841 tgacccgctt accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtcgacggc
    39901 cctgacggtt gctcaggttt gcggcgtcca acggttatgt aactactatt cggcgcgact
    39961 tacgccgctc cccgggcctg attcctccag ggaaagtaat catcggttgg cacaaatcac
    40021 gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca
    40081 tctgaatgac gtcggtctta ctgtctggga ctgtgtgatc attaatcaaa tcattggttt
    40141 tattggcttt caggcgcgga ccattgcgac atttcaggct tatctcgggc atccggtacg
    40201 ctggttaccc ggtctggaga tacaaaacta tgccgacgcg tcactgtttg ctgatgaatc
    40261 aatacgctgg cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc
    40321 aactacagaa ctttgccaac tggccgaaac actctctctc caccctattt cactttccct
    40381 tctcgaaaag ttgttaaaca gcacacgggt taatacacag ccggataatc agcttgcggc
    40441 gttgatatgc gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc
    40501 aaatgaatat aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg
    40561 ggctgaccgt cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc
    40621 acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac
    40681 gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat
    40741 cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc
    40801 cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc
    40861 ttaaacagca actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa
    40921 aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga
    40981 tgaaaaaagt catgcatggc gaccgaatta tcgcggttat ccacagtgaa aaagaacgtg
    41041 aatccgcaga gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc
    41101 agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc
    41161 cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcagttgccg
    41221 aaatgcgccg ccatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca
    41281 tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg
    41341 aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag
    41401 atctgaccgc gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg
    41461 cccttttcgc taaggcgttg ccggatggca aacttcagct gattgtggcg attgccgatc
    41521 caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca
    41581 ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct
    41641 gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg
    41701 atggcaccat tgaagataat atcgaattct ttgctgccac catcgaatcc aaagcgaagc
    41761 tggtgtatga cctggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg
    41821 aagcaattgc cgaacaagtc cgtttgttag cgcaaatttg ccaacgccgc ggcgagtggc
    41881 gtcataacca cgcactggta tttaaagatc gcccggatta ccgctttatt ctcggtgaaa
    41941 aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaatcgt atcgtcgaag
    42001 aagcgatgat tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg
    42061 gcatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc
    42121 tgaaaaccca cggtctgcat atcgatgccg aagaagtgct cacgctggac ggtttctgca
    42181 aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct
    42241 tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggagg
    42301 catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc
    42361 tgaaagcggt tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa
    42421 tggccgagcg tcgccgtctc aaccgaatgg cagaacgtga tgttggcgac tggttatacg
    42481 cacgcttcct gaaagacaaa gccgggaccg acacccgttt cgcggcggaa attgtcgata
    42541 tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg cgctattgcc tttattccgg
    42601 cacctttctt acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac
    42661 aaattaaagg tgaaacggct tacaaagtga ctgacgttat tgacgtcacc attgccgaag
    42721 tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc
    42781 attcctcatg aatgggccgt ttatttcccc gctccacctt cgtcatatcc cggcagattt
    42841 ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat
    42901 aaaaaaaaca atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt
    42961 taaccgcgga ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca
    43021 caacgttgta caactttctc ggatcgcaca atccatactg gcggttaacg gaaagcagcg
    43081 atgttttgcg cttttctacc accgaaacca cagaacctga acgtacgttg cagttatctg
    43141 ccgaacaggc tgctcgcatc agggaaatga cagtcatcac ttccagtctg atgatgagtc
    43201 tgaccgtcga tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg
    43261 aatgggctgg caacgcgtcg gcatggcatg acacaccggc ggttgctcgt gatttatcac
    43321 acgggctttc ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg
    43381 acagtcgggg gaatatccag cgcttcaatc ggttatgtga agattacaca gggttgaaag
    43441 aacacgacgt cattgggcaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg
    43501 catccaggcg caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt
    43561 ggataccaac acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg
    43621 gcagtggcaa aaacgagatt ttattaatct gttccggcac cgacattacc gaagagcgcc
    43681 gcgctcagga gcgactgcgc attctggcaa ataccgacag tatcaccgga ctgccgaatc
    43741 gtaacgcaat gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg
    43801 gggttgtgta tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt
    43861 ttggtgacca gctattacgc gacgtgtcat tggccatttt aagctgtctg gagcatgacc
    43921 aggttttggc gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa
    43981 gcgcgctgga agcaatggca tcacgaattt tgactcgctt acgactcccc ttccgcattg
    44041 gtttaattga agtttatacc agctgttcag tgggtatttc actctctccc gaacatggtt
    44101 cggacagcgc ggctattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg
    44161 gacgaggaca attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct
    44221 ggctggatac caacttgcgt aaagcactgg aaaacgatca gttggttatt cactatcaac
    44281 cgaaaatcac ctggcgtggc gaagtgcgca gtctggaagc tctagtacgt tggcagtcac
    44341 ctgaacgtgg gttgattcca ccgttggact tcatttccta cgccgaagag tcagggctca
    44401 ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg ccaggtggca aagtggcggg
    44461 ataaaggtat taacctgcga gtggcggtaa acatttctgc acgtcagctc gccgatcaaa
    44521 ccattttcac cgccctgaaa caggttctcc aggaactcaa ttttgaatac tgccctattg
    44581 atgttgaact gacggagagc tgcctgattg agaatgatga actggcactt tctgttattc
    44641 aacaatttag ccaactaggt gcgcaagtgc atctggacga ttttggtacc ggctactctt
    44701 cactttcgca actggcgcgc tttccgatcg atgcgattaa acttgaccag gtttttgttc
    44761 gagatattca caaacaacct gtatcgcagt cgctagtccg ggcgatcgtc gctgtggccc
    44821 aggcattgaa tcttcaggtg atcgctgaag gtgtggagag taaaaaggaa gatgcttttt
    44881 taaccaagaa cgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg
    44941 tcgccttcga acgttggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat
    45001 acttagcgat atattccgtc attatatatc gtattactgg atgataatta gccgttgctg
    45061 ttaaacactt ctcttttagc ggcaattttt gcattattcc ttttccactt ttgctataag
    45121 gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt
    45181 agatatcctt gtggcaggcg attaccactc tgcgatccat aatctcgaga tacttaaagc
    45241 agaactcttg cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat
    45301 ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt
    45361 atttagtatt ataacgttat aagaattaca gcgatgaatt cccgacaaca aacaattcta
    45421 cagatggtca ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt
    45481 tctgaagtta ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt
    45541 gcacatggct ttgcagtatc gcttgatagt gatgacgtcg aaactcgtat gatgagcaac
    45601 tatacgctga agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc
    45661 atctttatcg aaaatggcag cagcaatgcc ctgctcgccc ggactctggg cgagcagaag
    45721 aaaaatgtca ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct
    45781 tgtgaagtta ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggtcct
    45841 ttgacacgcc agtgcatcca acaggtgcat ttcagcaaag catttattgg tattgatggc
    45901 tggcaacctg aaactgggtt taccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc
    45961 gtgctggaaa aagagtgcga agcgatagtc ctgactgaca gctcgaaatt tggtgctgta
    46021 cattcatact ccatcggccc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata
    46081 cgcgccagcg atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt
    46141 taaccatttc ccttctctac ggatgatttg ctgttttgca aatcatccgc tctaagatga
    46201 ttcctggttg ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat
    46261 tatcagcgat ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcgg
    46321 atttcacgga ataatttcac caggcttatt cttagctatt atagttatag tgagcttact
    46381 tccgtgaatc ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc
    46441 ggctgttctg gcaattaccc tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg
    46501 agaccgtaac accgcaattg gcgcgggtgc aggtgcatta ggcggtgcag tactgaccga
    46561 tggcagtacg ctgggtacat taggtggtgc tgccgtcggt ggtgttattg gtcaccaggt
    46621 cggtaaataa tcgtaaataa atgtcgatta tcgactcgca attataatac gcttgattct
    46681 gttcagtacg aaataactga ataatacaca cagtaaaata ttcgaaagcc acggatatat
    46741 ccgtggcttt ttatgttaac cgcctgcgag ttttaccttc atccctttcg cttcaagtag
    46801 tgattttaat aaatcacgct tatcgccctg gatttcaata actccatctt taactgctcc
    46861 gccgcagccg cattttttct tcagttccgc tgcaagtttt gtcagttcgg catcatcgag
    46921 atcgacaccg gtaatcaagc aaacgccctt acctttacgt ccactggtct gacgctgaat
    46981 acgcaccaca ccgtcgcctt tagggcgtac aggggccgct tttggctcat caatacgtcc
    47041 ggtttccgtt gagtagacca gacggctgtt ggaatcactc atcatgcact ccgctgtaaa
    47101 gaggcgttga tcgctttcag cgtctgcgct ggatctaccg attgcgttat cgggcgacca
    47161 atcaccatat aatcaacacc agccgccgac gcctgttctg gcgtcataat gcggcgctgg
    47221 tcaccagctt cactcccctg cggacgaatg cccggcgtaa ccagtttgaa ctcctgacca
    47281 aatacctgtt taaagcgcgc agcttcctga gcagaacaca ccacaccatc aaggccacat
    47341 ttttgcgtca gtgctgccag acgttctgca taatctgcag gtgacagtgt cacgccaaga
    47401 tcggccaggt cgctggcttc catgctggtc aatactgtca cagcaatcaa aagcggtgca
    47461 tctttgccaa acggcaccag tgcctcacgc gctgcggtca tcatacgcgc cccaccggag
    47521 gcatgaacat tcaccatcca cacgcctaag tcagctgcag cagcgacagc gtgcgctgca
    47581 gtgttgggaa tatcgtgaaa tttcaggtcg aggaagatat caaaaccccg ttgttgaagt
    47641 tcgcgtacaa attgaggtcc aaacagcgta aacatctctt tgccgacttt cagacgacaa
    47701 tcgcgtgggt cgatcttgtc gacaaaggcc agcgcgtcat cacgattatg ataatcaagg
    47761 gcaacaacca caggagaatt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg
    47821 accagacctt cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt
    47881 ctacccgtgg aaataaaaaa ttaacagttg cgatttctcc cctggcaggc ttttctgcgc
    47941 ctgagttacc ggagcacaat ggaacatcaa caattagtat gttgtaacta aagtcggatt
    48001 tttttaaaaa ttacaggcca tcaagaccgc gaatcggttt aatggttgac caggcccgac
    48061 aagacggaca atgccagtag agagcgtatg cggtaaaacc acatttctgg cagcgataac
    48121 gaggcttact acgcaccttc tcgccaacca tgtcacgcag caccatcaga ctctctttgg
    48181 cacgcccttc ttccgcttca tttaagtggt aatccataag cttatggaac acacgcatgg
    48241 tcggatgacg ctgaagctga cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg
    48301 cttcgatgat gtccgcaagc atcagttcag catcggcacc ggtgttctct tccaccgcgc
    48361 gctgcaggaa ctctgcccat tcggcagttt tacccaactg ctggtagcag gtttgcagca
    48421 tttccagcgt ttcgctgacc agttctctgt cctgggatat cacgcgttgc agactttcga
    48481 cggctttggc gtattctcct ttcgccataa atacgcgtcc catcatgatg gatacgcggg
    48541 cgctgttttt atctgccgcc gcaccttttt ttaacaaggc catggcacga tcgagatcgt
    48601 cgctggccat atgctgcagg gctaactcac agtagaaatg ggcaatttcg acgcgctgtt
    48661 tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcgctgg
    48721 tagcctggta gatttgtagc aactgttgca gcgcgccaat gcggaagtca gtttcatcgg
    48781 tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcggccatg taatcacgcc
    48841 ccagttgttg aatcgccaac agacgctgtt cataggtcag cgaggcgctt tccattaggg
    48901 tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg
    48961 taaggtgggc ttcaacggta cccgtatcct ctttaagcat atcgagaaac aggtctaccg
    49021 ctttatcctg ttgattacta agcagaaagt taacccccgc tacgtaatca cgcgacaagc
    49081 ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg
    49141 cagcggctac aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgccg
    49201 ctgacgaatg cggcgctaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct
    49261 ttattttacg ttcagcgcgc gccagggaaa cacgaactcg cagccagaac agaccgcaaa
    49321 tcagccaacc gatagcaaac cccgcagcaa acaataccgc cagcaatgtg gaaatacggt
    49381 actccccttg cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacctaacg
    49441 tgaccgaaat cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac
    49501 ttcccgttat gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa
    49561 gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc attgttgatg atttaagggc
    49621 taaattcgga aaatcaacca gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt
    49681 tcttccacag gtggtgttaa tggcccacaa attcgttgcg caagccacgt tgccaccgcc
    49741 accagcgccc acgaaatcaa cgtagctacc accagatcgc gtggccaatg catcccgagc
    49801 agcaggcggc ttcccatgac tcccgttgcc cagaccaaca agatagcaat ggttaacgtt
    49861 cgacgacgcg gccacagcaa accaacggcc agcagagccc aactggcagc aaacatcgtg
    49921 tgaccggaag gaaaggcaaa ccccgtctct ttttgccagt gtgaacgcaa atattgtggg
    49981 atatttttct cttcagccaa ctgttctttc actagatttc cgcgttctgc tcgctttaaa
    50041 gtgtagaact catcaaccgg aatatgatgt attttttcca gccagataac aaaaggtcgt
    50101 ggttcctgga ctttgtcttt gatccaggat ttaactcctt gtcccacaag gattgccgcc
    50161 gccagaatgg caaataacat aatggcagcc ttaatgcgaa aacgcagaca ccagagaaac
    50221 cagccgaata aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc
    50281 caaaaagccg cttttagtag ccaactttgt tctccaggtt gccaacgcca gccagaaatc
    50341 catacggcta ctggcataac aagcaatagt gcagctccca ctgcggtacg tctggcaatc
    50401 gaacgcatgg cctctccttt ttgataagtc ccacaatcat aactgaaaat gccagttcca
    50461 ggaaaaattg acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac
    50521 cccttctcgt tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg
    50581 cagcttaaac gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg
    50641 ggatttgaag aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc
    50701 gggcataccc cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc
    50761 agcttgcgct gcgattgtgg cttccagctc gaagcggcac tgacgcaaat tgccgaagaa
    50821 ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa
    50881 atccgcgctt atgcactgca ggatcaaggt tacgataccg tagaggctaa ccaccagtta
    50941 ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc
    51001 gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagca
    51061 gggattaata ttgttgaacg cgtaccgttg attgtaggtc gtaaccccaa taacgaacat
    51121 tatctcgata ccaaagccga gaaaatgggt catttgctga acaaataacc ctcttgcatt
    51181 gtgtaattca tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactc gttacttcaa
    51241 catattacga atgacataat gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc
    51301 ggtgtcgata cgacaacggc agggtacgac ttcctggcta ccatccgcgc gcgtaaacgt
    51361 caccggaacc gtcgcgccgg gttgtagact ttgcagatcg acaatatcaa tcttctcttc
    51421 cccgattagc cctaacgttt tacgcgtcac gccttgcgga aattctagcg gcaggatgcc
    51481 catgccaatt aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat
    51541 tccaagcaga cgaggacctt ttgccgccca gtcacggctg gagcctgatc catactcttt
    51601 cccggcaatc accgccagcg gcgtttgctc ctgcttatag cgcatcgcgg catcataaat
    51661 agagatgacc tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat
    51721 ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg
    51781 ccgcgaaccg taggagttaa agtcttttcg ctcaacaccc cggccttgta gatatcgacc
    51841 cgctgggctg tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc
    51901 cccaagcatt gcgaggatcc gcgcaccgtg aatatcttcc acaggtgctg gtgttgcctg
    51961 catttcatca aagaaaggcg ataagcgaat ataggttgaa tcctcctgcc aaccgtaggt
    52021 atcggatcgt gcgacgttaa tttccttcca ctctgctatg ccttcaaaaa cttctgcgta
    52081 ctctttacgg aacatttctg tggagacttg ttctaccgca cgggcaattt cttgtgccga
    52141 tggccagata tctttcagat ataccggatc gcctttgcga tcgtgaccaa taggctcaga
    52201 tgcgaggttg atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc
    52261 cagccagttg gttttaacca gcggatggat acggccttca aagttacggt tgccggacag
    52321 cactgcaccg acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcagcgg
    52381 cccagagtta ccaatacagg tggtacaacc atatcccaca aggttaaacc ccagttcgtc
    52441 gagatacggt gtcaatttcg cttttgccag atagtcagaa acaactttcg agcctggtgc
    52501 cagcgacgct ttgacccacg gttgccgctt gaggcccaga gttacggctt ttttcgccag
    52561 caagcctgcg gccatcagca cacttgggtt agaggtattg gtgcacgacg ttatcgctgc
    52621 aatgaccaca gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg
    52681 ctggcgatct ttatgcgtgg catttacttc cagttcgtta ctggcggcaa atgcttttgg
    52741 tacatcgggc agtgcaacgc gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac
    52801 gtcattcata tccagttcta acgtactggt aaaaatcggt tcatcgcccg ggttacgcca
    52861 catgccctgc gctttggcgt atttttcgac caactcgacc tgatcttcac tgcgcccgct
    52921 taaacgcatg taatcgaggg tgacagcatc gattgggaag aagccacagg tggcaccata
    52981 ttctggcgac atattggcaa tggtggcgcg atccgccaac ggtagtgaat ccagaccatc
    53041 accataaaat tcgacgaatt tccccaccac gccatgtttg cgcagcattt gggtaacggt
    53101 gagaaccagg tctgtggcgg taataccttc acgtaatttt ccggtgagtt taaagcccac
    53161 tacgtccggg ataagcatgg aaaccggctg acctaacatt gcggcttctg cttcgatccc
    53221 ccccacgccc caccccagca cgccaaggcc gttgatcatg gtggtgtgcg agtcagtacc
    53281 aacgagtgta tccggataag caatccattc accgtcctgc aattcgctcc acactgcttt
    53341 gccgagatat tcgaggttaa cctgatggca aatgcctgtg cctggcggca cgacgctaaa
    53401 acgactgaac gcctgctttc cccatttcag gaacacataa cgttcgtggt tgcgctccat
    53461 ttccaggcgc acgttttctt caaatgcctc atcatcacca aaacgatcga cggtcaccga
    53521 gtggtcaatg accaggtcga ccggtgagag cgggttaacc tttgcagtat cgccgccgag
    53581 gcgtttaacc gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc
    53641 ctgcatcagc acccttgccg ggcggtaggc aatttcacgg tcagcatggg catttttcag
    53701 ccatcccgcc agcgcgtaga tatcctcttc ggtaaccgag ttaccatcct gccagcgcag
    53761 caggttttcg agcaaaactt tgagtgactt aggcaaacgg gtgatatcgc ccagtgattt
    53821 ggcagcaagc ggcaggctgt agtagtggta agttttatct ttggcctgca acgtgtcctt
    53881 actggcttct cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct
    53941 gattctcttc agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa
    54001 cccaaattga taaaagagga gaaagcttgt ggcaaaacaa atgaaaaccc cgccgcagcg
    54061 gggtcaggga tcagtgagta atgatccaga ggagttcggc ccagaagagc atcgacagag
    54121 aaaatgcgcc catccaggac caatatttca tatttgtgtt gatcataggc tacaccttta
    54181 acggttatta ctttgtgttg agataactca acctgttatt gctgcggtgt attaaaagcc
    54241 ctgtaattca ttaaaaatgg gtaataagaa tgatgataat tattgtttcg gctgatcatc
    54301 ttcagcaaac agttctataa aggcttgctg ctgcgatgtt agttgccagt cgtctggaat
    54361 aacgacggaa cattcccgtt tcgtttctgt ttcattgtca ctatgctggc ataatggtga
    54421 cgactccagt tgctcctgac cgaccatagt tatccccaca ctttccaaat cagcagtgcg
    54481 acaaccaccc agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt acgttttaat
    54541 tcaggatcgc ggcggggttc ctgatttcca gaaggcattg ctaacctcat ataatcgcca
    54601 tcacttatca gaaagacgtt gaacgattgg tgtaattaat catacgatga gacaaatcct
    54661 aaagaaactt tagtcaaaaa tccagcgaat ttacgaatcg tctccagtga ataattcaat
    54721 cttttgacct gaaatagata tttgaaaact ggagtttgcg aggcgggtaa ttagacacta
    54781 ctttacaggt aatttaccct ataaatatga gggataaaaa tattgtgcac ggtatacaaa
    54841 taagcaacaa aagagaatat attccggcac cttcgctaca taaaaggtgc cgaaaataac
    54901 gcaagaaatt atttttccgg cagttttata tctttaaaca tgacctcaat ttcttcatta
    54961 gagcgcaatg cgacagccgc atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga
    55021 atgaaatcat acatataact gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta
    55081 ctgtggctga agatatcgtg agcatcaacg cgcaccaggt cgggatcggc gaccggatcc
    55141 accgccatgc tggcaatgac ccctaccccc agccctaacc ggacgtaagt tttaatgacg
    55201 tcagcatccg ttgccgtgaa gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta
    55261 tccagctctg aacgtccggt aaagccgaag gtatatgtca ccaacggata ttgcgccagt
    55321 tcttcaatgg taatggcttt tttgcctgcc agcgggtgat ccggagtgac tacaatagcc
    55381 cgattccagt ggtagcacgg taacatcact agatcttcat acagatgcaa cgcttctgtg
    55441 gcgatagcga aatcggcatt gcctttagag acagcatcag caatttgtgt cggcgagccc
    55501 tggtgcatat gcaaagaaac gcggggataa cgctcaataa agcctttgat gacgtttggt
    55561 aacgcgtagc gtgcctgggt atgcgtggtg gcgatataca gcgagccttt atccggccag
    55621 gtgtgctctc cggcgaccga ttttatggca tcaactttcg acaggacttc acgagcgata
    55681 cgaattattt cttgtcctgc tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa
    55741 atttgaatgc ctagctcgtc ttccagcatt ctgacttgtt tactgatccc gggttgtgat
    55801 gtgtaaagtc cttccgccgt tgatgagaca ttcaggttat gattgaccac ctcaacaata
    55861 tagcgaagtt gttgtaattt catgttaaac catccactta gacgccatca gaacatcgtt
    55921 ttttcgtcac acctggcggg tgactaatac gatttaataa taaaaaaggt gctaactata
    55981 accactatat catttatagc cttagtgtat aggtgtgaag aaaaaataat atgcaataaa
    56041 aaagggccgc tttcgcgacc ctttgtttat aaaaacctga cagaattaaa ggttattttt
    56101 ttccttcaac ccatttgcca tcaacataaa atgctgacca gccagtcgcc tttccgtctt
    56161 tttccgaaga gacatattgc tgtttggttt tacggctaaa gcgaaccatg gtcttattac
    56221 cttccggatc ctgctgtggc gcatcggcca gataacgcag tttttccggc agacggtcgc
    56281 ggaagcgata aagctcttcc accagtggcg cacgtgtttc acgcgatttc gggaaggtat
    56341 tggccgccag gaacacaccg gcagcaccgt cacgcagcac gaaataagca tctgattttt
    56401 cgcacggcag ctccggtaat ggcaccggat cttctttcgg tggtgccact tcgccgttac
    56461 gtaaaatctt acgcgtgttt ttacactctt cgttagtgca ggccatgtat ttaccgaaac
    56521 gccccatttt caggtgcatt tcagagccac atttttcaca ctcaacgatc gggccgtcat
    56581 aacctttaat gcggaattcg ccctcttcga tctcgtaacc gtcgcaggtt gggttattac
    56641 cacagacatg caacttacgt ttcggatcga tgagatagct gtccatcgcc gtgccgcatt
    56701 tcgggcaacg acgttttgcg cgcagcgcgt tggtttcagc gtcttcgcct tccagcacgt
    56761 tcagcacttc gttttccggc accaggttaa tggtggtttt gcaacgctct ttcggcggca
    56821 gcgcatagcc agaacagcca aggaataccc cggtgctcgc tgtgcgaatc cccattttgc
    56881 gaccacaagt cgggcagtca atgctggtca gaaccatctg gttcgggcgc atccccccct
    56941 cttccggatc tttttcagct ttatctaact gctgggtgaa atccgagaag aagtgatcca
    57001 gtacagcttt ccactctgct tcgtgatttg ccacctggtc gaggctgttt tccatctgtg
    57061 cggtgaagtc gtagttcatt aactcacgga aattttcttc aaggcgatcg gtgacaattt
    57121 cacccatttt ttccgcgtag aaacgacgat tttctactcg cacatagcca cgatcctgaa
    57181 tggtcgaaat gatcgacgca taggtagacg gacgaccgat gccgcgtttt tccagctctt
    57241 taaccaacga tgcttcactg aaacgcgctg gcggcttggt aaagtgctgg gctggtgtca
    57301 gttcaacgag tgtcaaagca tcgcctttat cgactgccgg taagatacga tcttcatcgc
    57361 ctttacgcag cgcaggcatc actttcgtcc agccatcaaa gcgcaaaata cgaccgcgtg
    57421 ctttcaggcg gaaatcgccc gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg
    57481 tcatctggca ggcaacgaac tgacgccaga ttaactggta cagtttctgc gcatctgctt
    57541 ccatatcctt cagcgattcc gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg
    57601 cttcctgtga gttttctttg ctggcgtact gattcggact ttccggcaga tatttcttac
    57661 caaaattatc gctgatataa ccgcgaacca tatttaccgc gtcctgactc aggttggtgg
    57721 agtcggtacg catgtatgtg atatagccag cttcatacaa acgctgcgcc atcatcatgg
    57781 tttttttcac gccaaaacca agacgggtgc tggcagcttg ttgcagcgta gaggtaataa
    57841 aaggagcgcc aggtttactg gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg
    57901 ctttttccag cagacttacc gcagcctgag tttgttcttt gttgaccgga cggaacggtt
    57961 tgtcgttctg atgagtcacc tgcaacgcca acgcttcacc agatggcgtg gtcgtgctgg
    58021 catcgacttc ccagaactct tccggtacga acgctttaat ttcacgctca cgctcgacca
    58081 ccaggcggac cgccaccgac tgcacacgac cggcagacag accacgagcg atctttttcc
    58141 acagcagcgg cgaaaccata taccccacca cgcggtccat aaagcgacgc gcctgctggg
    58201 cattaacacg atcaatattc agctcacctg gtttgttaaa tgcctggcgg atcgcgtttt
    58261 tagtaatttc gttaaacacc actcggctat agcgcgcatc atcaccacca atcacttccc
    58321 gcaggtgcca tgcaatggct tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt
    58381 cggctttttc agccagttgt ttcagttcag agacgacctt ctctttacca ggcaacactt
    58441 catagtgcgc ctcccaattg tgccacgggt caacccccat acggttgacg agagcgccac
    58501 gttcatcctt tttaggcttt ttagccgtct tggtggaggt agagtcggca ctctttttgg
    58561 cagctgagcc actggtcggc aaatcgcgga tgtgaccgac gctggatttc accacgtagt
    58621 cactacccag atacttgttg atcgttttgg cttttgccgg ggactcaacg atgacaagag
    58681 ctttacccat attcacctta cctaatttga ttcttccagg aatgcgtcgc acgttgataa
    58741 accttccact ggcaacgagt taccgatatg gactcgctct ctgcggatat caaccccttg
    58801 tcgtgactct gttcacgaaa attcaggtga ctgagttaac aggtcttggc ctacaggcgc
    58861 tatagcacga cggaattttg gtcgaatgtc aagcaaatct attgccagat tgacgaaagc
    58921 gtcacactgt acctgataaa atccgatacg caactttatt agcatgcaaa atccaaagtt
    58981 gccagtaagg cgctcttgcc accagcgtgg tatatttgtc tgcaagccat gcctggcgta
    59041 gactaatgtc cttgtttaag gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg
    59101 agacgctgtt gaaagaggcc aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga
    59161 ttgaagccac cggcgtcacg cagcgtaatg gcgtgctggt atttaccggc gactactttt
    59221 tagatgaaca aggattgcca acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc
    59281 tggcccatgt gctttcagaa aaatatcacc tggttgatta aagcaaaacg cgaggtctta
    59341 gcctcgcgtt tgtcttttac atcaatggct tttgaccccg ctgccaccag cgtagcaaca
    59401 atcgatcggc gctctctgcc gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca
    59461 tatagcgtac attgaccact tcacggcctt ccatcaggct aagaataact tcatcactgg
    59521 tgttgatttc atcaaccagg cctttctcta ccgcctgttg tccgtaccag tgttcacccg
    59581 ttgccacctg ttcaatatcc agagacggac gcatacgctt cacaaaatct ttaaataact
    59641 gatgcgtttc gttcagctct tcgcggaatt tctcccgccc ttcttcggta ttttcaccca
    59701 gcaacgtcag cgtgcgctta tactgcccgg cggtgtgcag ttcgatatca atatctttgc
    59761 ttttcaggaa gcggttaaag ttgggcattt gcgccaccac gccaatagaa cccacaatag
    59821 caaacggtgc ggaaacaatt ttgtctgcca cacaggccat catgtaaccg ccactggcag
    59881 caactttgtc taccgtaaca gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg
    59941 aagccgccag cccgtaacca tgcaccatgc caccagggct ttccagacgt agcacaacct
    60001 gatcctgcgg tttgaatgct gcgagtaccg ccgttatctc ttcacgtagc gagttcactt
    60061 catgggcgtc catgctgcct ttaaaatcca gcacccagac gcgaggttta ctgtcagttg
    60121 ccacctcgcc cagtttggct ttcgcttttg ctgctttcgc ttcttgtttg tgctttttct
    60181 tctgcgtttt gtgccactgt ttttgctgat gagtgtccat cagcgccgca gccagttctt
    60241 ctttcatctc cttatactgt tcgctgatat tgttgacccg taactcgcca cgctggcgtt
    60301 tattacgttg agcaacattg acaataatgg cggcaatcgc cgcaatcgct agcacaacgg
    60361 taacgatttt cgccaaaaac aaaccatatt cagacaacaa ttccacgagt ccccaccttg
    60421 gttaaacaac gctttggctc acagtttaca acaggcatgt taagccgtct cgcagagatg
    60481 ttattgcagc gaaagtatga caaagtatcc attttgcggt gttatcgccc caggctgatt
    60541 gaaatgccct tctgtttcag gcataaagcc ccaaagtcat aaagtacact ggcagcgcga
    60601 cagacaacgt cgttgaggag tagccatgca ttaccagcca aaacaagatt tactcaatga
    60661 tcgcattatc ctggtgacgg gagccagcga tggtattggc cgtgaagccg cgatgacgta
    60721 tgcacgctat ggtgcgacag tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt
    60781 agccagccac ataaacgaag aaactggacg tcagccacag tggtttattc tcgatttgct
    60841 gacctgcacg tccgaagatt gccaacaact ggcacagcgc attgccgtta attatccgcg
    60901 tctggatggt gttttgcata atgccggatt gctcggcgat gtttgcccaa tgagcgaaca
    60961 aaatccgcag gtctggcagg acgtcatgca ggtcaacgtt aatgccacct ttatgctcac
    61021 ccaggcactg cttcctttat tactcaaatc ggacgccggt tcactggtct ttacttcatc
    61081 aagcgttgga cgtcagggac gagccaactg gggcgcatat gcagcgtcga aatttgccac
    61141 cgaagggatg atgcaggtac tggccgatga atatcagcag cgcctgcgcg tcaactgcat
    61201 taacccaggc ggtacgcgca ccgcaatgcg ggccagcgcc ttcccgaccg aagatccaca
    61261 gaaacttaaa acacccgctg atatcatgcc gctctacctc tggctgatgg gcgatgacag
    61321 ccgccgtaaa accggcatga cctttgacgc ccaaccgggc cgtaaaccag gaatttccca
    61381 atgagtgatg aacgctacca acagcgtcag cagcgagtga aagaaaaagt cgatgctcgt
    61441 gtggcccagg cccaggatga acgcggtatt atcatcgttt ttaccggcaa tggaaaaggc
    61501 aaaaccaccg cggcatttgg tacggcaact cgtgcagtag gccatggcaa aaaagtgggt
    61561 atcgtgcagt ttattaaagg cacctggcct aatggcgagc gcaatctact ggagccacat
    61621 ggcgttgagt ttcaggtgat ggcaacgggc tttacctggg atacacaaaa tcgcgaatct
    61681 gataccgcgg cctgccgtga agtctggcaa catgccaaac gaatgctggc cgatccttca
    61741 ctggatatgg ttttgctcga tgagctgact tatatggtgg cgtatgatta tttgccactt
    61801 gaagaagtgg tgaaggcgtt aaatgaacgt ccacatcagc aaaccgtcat tattacgggt
    61861 cgtggttgtc atcgggatat acttgaactg gcggatacgg taagtgaatt acgccccgtg
    61921 aaacatgcgt ttgatgccgg tgtaaaagcg cagataggga ttgactacta agtgaaagca
    61981 cctcaaaaga agcctcttga ggtgctttaa tcatgattat attcaagcgg caatagcgaa
    62041 attcagaata cgtgtaatct cattcaaatg tcccggatat gcttcatgat tctcagcgtc
    62101 aacatcttca ataaataata tcagttcagt aattaacgca ttagcatcgt cataatttat
    62161 cccggctaac tctggcgaag tttcagtacc atagttaatc gtcaggttgt tcataactgt
    62221 gaggatatgg ataaaatatt tatgacaaac attacatgtg gttttattac ccaaagtatt
    62281 atacagcata tcaagtaatg ccagtattct acggttttgc acatcgttca taaaaagcct
    62341 cctataacat tattccgctg gcaataagta attttgccag cccctcagac gcctttaacc
    62401 cactaatgac ctgtgcgcgt aatatttcat ttaatctttt ttgttcagct gccgtgcgtg
    62461 ttttattaac gtataagctt tttagataat cacccttatc tataaaggca tttaaagcct
    62521 caatcgcagg catggcaggt gccgatgcct gtagtctggt ttcattcaca ttgattactg
    62581 aacttagaaa ctccttcctg tttttcagag tgttaaactc tgtattctta gagtttttag
    62641 atgctg
//
LOCUS       DARNVM010000007        61650 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.007, whole genome shotgun sequence.
ACCESSION   DARNVM010000007 DARNVM010000000
VERSION     DARNVM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.007"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            118..744
                     /locus_tag="SI621_000425"
     CDS             118..744
                     /locus_tag="SI621_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HEI4063181.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            765..2867
                     /locus_tag="SI621_000426"
     CDS             765..2867
                     /locus_tag="SI621_000426"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HEI4063182.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2880..3518
                     /locus_tag="SI621_000427"
     CDS             2880..3518
                     /locus_tag="SI621_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HEI4063183.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDHADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIES
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3582..4250
                     /locus_tag="SI621_000428"
     CDS             3582..4250
                     /locus_tag="SI621_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEI4063184.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHYFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4247..5032
                     /locus_tag="SI621_000429"
     CDS             4247..5032
                     /locus_tag="SI621_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HEI4063185.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWFVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5036..5848
                     /locus_tag="SI621_000430"
     CDS             5036..5848
                     /locus_tag="SI621_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063186.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5860..7464)
                     /locus_tag="SI621_000431"
     CDS             complement(5860..7464)
                     /locus_tag="SI621_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HEI4063187.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPGWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDKNPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7590..7895)
                     /locus_tag="SI621_000432"
     CDS             complement(7590..7895)
                     /locus_tag="SI621_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEI4063188.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLEPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8003..8079)
                     /locus_tag="SI621_000433"
     tRNA            complement(8003..8079)
                     /locus_tag="SI621_000433"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8043..8045),aa:Val,seq:gac)
     gene            complement(8084..8160)
                     /locus_tag="SI621_000434"
     tRNA            complement(8084..8160)
                     /locus_tag="SI621_000434"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8124..8126),aa:Val,seq:gac)
     gene            8468..9724
                     /locus_tag="SI621_000435"
     CDS             8468..9724
                     /locus_tag="SI621_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HEI4063189.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGVTQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRV
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITDAATQVHLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9765..11138)
                     /gene="mdtK"
                     /locus_tag="SI621_000436"
     CDS             complement(9765..11138)
                     /gene="mdtK"
                     /locus_tag="SI621_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HEI4063190.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11353..11994
                     /gene="ribE"
                     /locus_tag="SI621_000437"
     CDS             11353..11994
                     /gene="ribE"
                     /locus_tag="SI621_000437"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEI4063191.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12034..13182)
                     /gene="cfa"
                     /locus_tag="SI621_000438"
     CDS             complement(12034..13182)
                     /gene="cfa"
                     /locus_tag="SI621_000438"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEI4063192.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIHVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13473..14684)
                     /locus_tag="SI621_000439"
     CDS             complement(13473..14684)
                     /locus_tag="SI621_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HEI4063193.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14797..15729
                     /locus_tag="SI621_000440"
     CDS             14797..15729
                     /locus_tag="SI621_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4063194.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15726..16751)
                     /gene="purR"
                     /locus_tag="SI621_000441"
     CDS             complement(15726..16751)
                     /gene="purR"
                     /locus_tag="SI621_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HEI4063195.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17050..17139
                     /gene="ynhF"
                     /locus_tag="SI621_000442"
     CDS             17050..17139
                     /gene="ynhF"
                     /locus_tag="SI621_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HEI4063196.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17305..18474
                     /locus_tag="SI621_000443"
     CDS             17305..18474
                     /locus_tag="SI621_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063197.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18620..19201)
                     /gene="sodB"
                     /locus_tag="SI621_000444"
     CDS             complement(18620..19201)
                     /gene="sodB"
                     /locus_tag="SI621_000444"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEI4063198.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19329..20156)
                     /gene="mepH"
                     /locus_tag="SI621_000445"
     CDS             complement(19329..20156)
                     /gene="mepH"
                     /locus_tag="SI621_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HEI4063199.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20491..20838
                     /gene="grxD"
                     /locus_tag="SI621_000446"
     CDS             20491..20838
                     /gene="grxD"
                     /locus_tag="SI621_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HEI4063200.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20889..25505)
                     /locus_tag="SI621_000447"
     CDS             complement(20889..25505)
                     /locus_tag="SI621_000447"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEI4063201.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDAQLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAVHFE
                     STSGATSNRVQNSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECSARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKDELLAPEIFQALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25598..26245)
                     /gene="rnt"
                     /locus_tag="SI621_000448"
     CDS             complement(25598..26245)
                     /gene="rnt"
                     /locus_tag="SI621_000448"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEI4063202.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26348..26755)
                     /gene="gloA"
                     /locus_tag="SI621_000449"
     CDS             complement(26348..26755)
                     /gene="gloA"
                     /locus_tag="SI621_000449"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEI4063203.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26836..27933)
                     /gene="nemA"
                     /locus_tag="SI621_000450"
     CDS             complement(26836..27933)
                     /gene="nemA"
                     /locus_tag="SI621_000450"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HEI4063204.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27970..28569)
                     /gene="nemR"
                     /locus_tag="SI621_000451"
     CDS             complement(27970..28569)
                     /gene="nemR"
                     /locus_tag="SI621_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HEI4063205.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIVLLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28672..28911
                     /locus_tag="SI621_000452"
     CDS             28672..28911
                     /locus_tag="SI621_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEI4063206.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28960..29856
                     /locus_tag="SI621_000453"
     CDS             28960..29856
                     /locus_tag="SI621_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEI4063207.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29937..30458
                     /gene="sodC"
                     /locus_tag="SI621_000454"
     CDS             29937..30458
                     /gene="sodC"
                     /locus_tag="SI621_000454"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HEI4063208.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30459..32471)
                     /locus_tag="SI621_000455"
     CDS             complement(30459..32471)
                     /locus_tag="SI621_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEI4063209.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSGARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32471..33328)
                     /locus_tag="SI621_000456"
     CDS             complement(32471..33328)
                     /locus_tag="SI621_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEI4063210.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33331..33567)
                     /locus_tag="SI621_000457"
     CDS             complement(33331..33567)
                     /locus_tag="SI621_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEI4063211.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33768..34202
                     /gene="slyA"
                     /locus_tag="SI621_000458"
     CDS             33768..34202
                     /gene="slyA"
                     /locus_tag="SI621_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HEI4063212.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34249..34716)
                     /gene="slyB"
                     /locus_tag="SI621_000459"
     CDS             complement(34249..34716)
                     /gene="slyB"
                     /locus_tag="SI621_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HEI4063213.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            34990..36099
                     /gene="anmK"
                     /locus_tag="SI621_000460"
     CDS             34990..36099
                     /gene="anmK"
                     /locus_tag="SI621_000460"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEI4063214.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36197..36526
                     /gene="mliC"
                     /locus_tag="SI621_000461"
     CDS             36197..36526
                     /gene="mliC"
                     /locus_tag="SI621_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HEI4063215.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36585..37241
                     /gene="pdxH"
                     /locus_tag="SI621_000462"
     CDS             36585..37241
                     /gene="pdxH"
                     /locus_tag="SI621_000462"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEI4063216.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37370..38644
                     /gene="tyrS"
                     /locus_tag="SI621_000463"
     CDS             37370..38644
                     /gene="tyrS"
                     /locus_tag="SI621_000463"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEI4063217.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38706..39566
                     /gene="pdxY"
                     /locus_tag="SI621_000464"
     CDS             38706..39566
                     /gene="pdxY"
                     /locus_tag="SI621_000464"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HEI4063218.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39610..40215)
                     /gene="gstA"
                     /locus_tag="SI621_000465"
     CDS             complement(39610..40215)
                     /gene="gstA"
                     /locus_tag="SI621_000465"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEI4063219.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40320..41822)
                     /gene="dtpA"
                     /locus_tag="SI621_000466"
     CDS             complement(40320..41822)
                     /gene="dtpA"
                     /locus_tag="SI621_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HEI4063220.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42433..43068)
                     /gene="nth"
                     /locus_tag="SI621_000467"
     CDS             complement(42433..43068)
                     /gene="nth"
                     /locus_tag="SI621_000467"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEI4063221.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKIDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43068..43763)
                     /gene="rsxE"
                     /locus_tag="SI621_000468"
     CDS             complement(43068..43763)
                     /gene="rsxE"
                     /locus_tag="SI621_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HEI4063222.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHLTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43767..44387)
                     /gene="rsxG"
                     /locus_tag="SI621_000469"
     CDS             complement(43767..44387)
                     /gene="rsxG"
                     /locus_tag="SI621_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEI4063223.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(44391..45449)
                     /gene="rsxD"
                     /locus_tag="SI621_000470"
     CDS             complement(44391..45449)
                     /gene="rsxD"
                     /locus_tag="SI621_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HEI4063224.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHDIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45450..47576)
                     /gene="rsxC"
                     /locus_tag="SI621_000471"
     CDS             complement(45450..47576)
                     /gene="rsxC"
                     /locus_tag="SI621_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEI4063225.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGSEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIHQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAVREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAKPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEKVDPRKAAVE
                     AAIARAKARKLEQQKANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(47569..48147)
                     /gene="rsxB"
                     /locus_tag="SI621_000472"
     CDS             complement(47569..48147)
                     /gene="rsxB"
                     /locus_tag="SI621_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEI4063226.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPTRMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48147..48728)
                     /gene="rsxA"
                     /locus_tag="SI621_000473"
     CDS             complement(48147..48728)
                     /gene="rsxA"
                     /locus_tag="SI621_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEI4063227.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48805..49245)
                     /locus_tag="SI621_000474"
     CDS             complement(48805..49245)
                     /locus_tag="SI621_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HEI4063228.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49331..49546)
                     /gene="ydgT"
                     /locus_tag="SI621_000475"
     CDS             complement(49331..49546)
                     /gene="ydgT"
                     /locus_tag="SI621_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HEI4063229.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49819..49944)
                     /gene="blr"
                     /locus_tag="SI621_000476"
     CDS             complement(49819..49944)
                     /gene="blr"
                     /locus_tag="SI621_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HEI4063230.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50187..51227
                     /locus_tag="SI621_000477"
     CDS             50187..51227
                     /locus_tag="SI621_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEI4063231.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGQRLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51263..52264)
                     /gene="add"
                     /locus_tag="SI621_000478"
     CDS             complement(51263..52264)
                     /gene="add"
                     /locus_tag="SI621_000478"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEI4063232.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVSFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52368..53540)
                     /gene="malY"
                     /locus_tag="SI621_000479"
     CDS             complement(52368..53540)
                     /gene="malY"
                     /locus_tag="SI621_000479"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HEI4063233.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWAEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53550..55142)
                     /gene="malX"
                     /locus_tag="SI621_000480"
     CDS             complement(53550..55142)
                     /gene="malX"
                     /locus_tag="SI621_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HEI4063234.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKTNNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGVPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55317..56345
                     /gene="malI"
                     /locus_tag="SI621_000481"
     CDS             55317..56345
                     /gene="malI"
                     /locus_tag="SI621_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HEI4063235.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEEAHSRNLII
                     PARLIAPK"
     gene            56457..57224
                     /gene="hdhA"
                     /locus_tag="SI621_000482"
     CDS             56457..57224
                     /gene="hdhA"
                     /locus_tag="SI621_000482"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HEI4063236.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57445..58035
                     /gene="uidR"
                     /locus_tag="SI621_000483"
     CDS             57445..58035
                     /gene="uidR"
                     /locus_tag="SI621_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HEI4063237.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58425..60236
                     /gene="uidA"
                     /locus_tag="SI621_000484"
     CDS             58425..60236
                     /gene="uidA"
                     /locus_tag="SI621_000484"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEI4063238.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQLWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDFYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60233..61606
                     /gene="uidB"
                     /locus_tag="SI621_000485"
     CDS             60233..61606
                     /gene="uidB"
                     /locus_tag="SI621_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HEI4063239.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
BASE COUNT        15503 a        15254 c        16113 g        14780 t
ORIGIN      
        1 tatcgggtta gggaaaatat ctggtgttgg ggacagagtc agcagcgtat tagttgaatg
       61 cttaatatca gactgctgaa ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg
      121 aacccggttg atcgtccact attagatatt ggcttaacgc gattagaatt cttgcgtata
      181 tcaggaaaag gccttgctgg tctaacaatt gcccctgcgt tactgtcact tttgggttgc
      241 aaacaggaag atattgatag cggcacagta gggttgataa atacacccaa aggggtgctg
      301 gtgacccagc gagcgcgatg cacgggttgc caccgctgtg aaatctcttg taccaacttc
      361 aacgatggct cagtagggac attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt
      421 ggcgacaacg gggttggctc tggcggcggc ctgtatggcg atctcaacta taccgcggac
      481 acctgccgac aatgcaaaga gccgcaatgc atgaacgtct gcccgattgg tgcgattacc
      541 tggcagcaga aagaaggctg tattaccgtc gatcataaac gctgtattgg ctgtagcgcc
      601 tgtactacgg catgcccgtg gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg
      661 aaatgtgtgt tatgcggtga atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc
      721 gagtggaaag atattactgt gtgaatcttg caaaggaaaa aatcatggct aacggttgga
      781 caggtaatat attaagagtc aatctcacga caggaaatat taccctcgaa gattccagta
      841 agtttaaaag ttttgttggt ggtatgggct tcggctacaa aattatgtat gacgaagtac
      901 cgccaggcac gaaacctttc gatgaagcga ataaattagt ctttgctacc ggcccattaa
      961 ctggatctgg tgccccctgt agttctcgcg taaatatcac ctcactttct acttttacca
     1021 aaggaaattt agtcgtcgat gcccatatgg ggggcttttt tgcagcgcaa atgaaattcg
     1081 ctggatacga cgtcattatt atcgaaggga aagcgaaatc accggtatgg ctgaatatta
     1141 aagatgacaa agttagcctg gaaaaagcgg atttcttatg gggaaaaggt acgcgcgcaa
     1201 ctacggaaga gatttgtcgc ttgaccagcc ctgaaacctg tgtggcggct attggtcagg
     1261 ctggggaaaa ccttgttcct ctctctggca tgttgaatag ccgtaaccac agcggtggtg
     1321 cgggaactgg cgcaataatg ggttcgaaaa acctgaaagc gattgcggtt gaagggacga
     1381 aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct caacgattac atgatgactg
     1441 aacttatcgg tgcgaataac aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt
     1501 attcagatcc caagtcacgt tggacagcac gtaaagggct gttttggggc gcggctgaag
     1561 gtggtccgat tgaaacgggg gaaattccgc caggcaatca gaacacggtc ggctttcgta
     1621 cctataaatc cgtttttgac ttaggtccgg cggcagagaa atacacagta aaaatgagcg
     1681 gctgccactc ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt
     1741 ttggcgtgcc tggcaccggt ggtaacactt gtgtagcaaa ctttgtccat accaccatct
     1801 tcccaaacgg gccgaaagat tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg
     1861 ttggtctgaa tctattcgat gactacggcc tatggtgtaa ctatgggcag ctgcatcgcg
     1921 actttactta ttgttacagc aaaggggtgt tcaagcgtgt tctaccagct gaagagtatg
     1981 cggaaattcg ctgggatcaa ctggaagcgg gtgacgttaa cttcattaaa gatttttact
     2041 accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tatgccatcg
     2101 cagaacgctg gaatttgggt gaagagtact ggggctacgc gaaaaataaa ctctggtcgc
     2161 cgtttggcta tccagttcac catgccaatg aagcgtcagc gcaagtcggt tccattgtta
     2221 actgtatgtt caaccgtgac tgcatgacgc atacccatat caattttatt ggttccggtt
     2281 tgccattgaa actgcaacgt gaggtggcga aagagctttt tggttctgaa gatgcttacg
     2341 atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat taaatatgct aagtggtcgc
     2401 tgttgcgggt ctgtttgcat aacgccgtca cactgtgcaa ctgggtctgg ccaatgaccg
     2461 tttcgccgct gaaaagccgt aattatcggg gcgatctggc gcttgaagcc aaattcttca
     2521 aagcgatcac tggcgaagag atgactcagg aaaaattaga tttagctgca gagcgtatct
     2581 ttacgttgca tcgtgcctac acggtaaaac tgatgcaaac caaagatatg cgtaatgaac
     2641 acgatcttat ctgttcctgg gtattcgaca aggatccgca gatcccggtc tttactgaag
     2701 gtactgacaa aatggaccgt gacgatatgc atgcttcgct cacgatgttc tacaaagaaa
     2761 tgggctggga cccacagctt ggttgtccga cccgtgaaac attgcagcgt ctggggctgg
     2821 aagatattgc cgccgatctg gcagcacaca atctactgcc tgcgtaagga atgggtgaga
     2881 tgaaccatca ggatgaattg ccgttagcga aagtcagcga agttgatgag gcgaagcggc
     2941 aatggttgca ggggatgcgt caccccgttg atacggtaac cgaaccagaa cctgctgaaa
     3001 tcctggcaga gttcattcgt cagcattctg cggctgggca gctggtggcg cgcgccgtgt
     3061 ttctttcgcc accctattcg gttgcagaag aggaactgtc agttttgctg gaaagcatta
     3121 agcagaacgg agaccacgcg gatatcgctt gtttgacggg aagccaggat gattactatt
     3181 actcaactca ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg
     3241 acatttgtcg cgccattgca cacgccgttc gttttgagtg ccagacgtat cctcgtcctt
     3301 acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag atcgaatccg
     3361 cgatcgccgc gatggatatt gcacctgaat atgctgatat acggcaggtg gagtcatcga
     3421 cggcggtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggcg tatggcttat
     3481 gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcagggag atgtcatgga
     3541 tagggtggac agttgcagcc actgcattag gggataatca gatgtccttc actcgacgca
     3601 aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag
     3661 cgaacacgag gcaagaaaag aaagttcggt atgccatgat tcatgacgag tcacggtgca
     3721 atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa
     3781 gccgtttatc gatagcacac attccagtca ccgataacga caacgaaacg cagtatcact
     3841 attttcgcca gtcatgccag cactgtgaag atgctccttg catcgacgta tgcccgacag
     3901 gggcgtcgtg gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag tgcatcggct
     3961 gcagttattg cattggcgct tgtccttacc aggttcgcta ccttaaccct gtcactaaag
     4021 tggcagataa atgtgatttc tgcgctgagt cccgactggc aaaaggcttt ccgcccattt
     4081 gcgtcagcgc ctgtccggaa cacgcattga tctttggtcg cgaagatagc ccagaaattc
     4141 aggcgtggtt gcaggaaaat aaatactatc aatatcagct acctggtgcc gggaaaccgc
     4201 acctgtatcg ccggttcggt caacatttga ttaaaaagga aaatgtatga acccgtcgca
     4261 acatgctgaa cagtttcaga gccagttggc gaactatgtg ccacagttca ctccccaatt
     4321 ttggccggtg tggttgatca ttgccggact attgctggta gggatgtggt ttgtgctggg
     4381 gctgcatgcc ttgcttcgtg ctcgtggcgt gaagaaatca gccaccgacc atggtgagaa
     4441 ggtttatctt tacagcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt
     4501 attgttgctg gcaagtgggc tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa
     4561 aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctggcatgtt ggctcggctt
     4621 tgttctgatc aatgccgttg ggggtaatgg tcaccattat cgcattcgtc gtcaggggtg
     4681 gctggagcga gcggcaaaac aaacgcgatt ctatttgttt ggcattatgc agggggaaga
     4741 acatcctttc ccggcaacaa cacagtctaa atttaatccc ttacagcagg ttgcctatat
     4801 tggtgtcatg tatggattgc tgccgttgtt actattgacg gggctgctgt gtctctatcc
     4861 gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc
     4921 tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg
     4981 tacgccacac gaaaccttta aaagcatggt cgatggctat caccggcact aacgaatgat
     5041 cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggttcct
     5101 gcgccatttc ccacgcggcg gtagttatgc cgatattcat catgcactga ttgaggaagg
     5161 atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga
     5221 aaacttcgcg catcaggaag tttcctctat gcaaaaattg gccacagatc ccggagagag
     5281 accattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga
     5341 taatgccaga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt
     5401 gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgaac gcgcacgggt
     5461 tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagtagtc ggattgcgaa
     5521 taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg
     5581 tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaaca gcggcgacaa
     5641 tgtacacatc attgccagcg gtgaagattc aacaatagtg agcacgggcg tggtcgactc
     5701 aattattctc gggccagggg gaagcgccgc actggcatat catgacggcg aaagagttcg
     5761 cttcgccgtc gccattgaag gtgaaaacaa tattcgagcc ggagtccgct atcgtcttaa
     5821 tgaacagcat cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgcct
     5881 acgtgcacgg gaggcaggtt ttacgacagc ccgagccatc gcctcaccaa tttctgcaca
     5941 tgccgtaagt ccctgaacga aaggctggtc gtgcattaac cagggcaacg cgccgaacgc
     6001 tacgcgccca cgaatatctt cgggttgctg taacgtataa tcttcgccaa catcagggtt
     6061 tttatcccct gttttctgta attgttcgcg tagtccaggg aaaggaatat ctttcacttt
     6121 aagcggacgc tgcccacggg catcaataaa aacgtcaaac gagtagctgt tgtcttccgt
     6181 tttcaggacg gtgcgcgact cattaatttc catttcgtag tcttcaccga gggcgagaat
     6241 atgaatgatt cccgcttcac gtaaggccag tagtcgacga atagactctg aagggattgc
     6301 cgcataatta tcgatgaaaa cccgggcaag gcctttactg aaccgtttat ggtcgtgttc
     6361 attcagatgt ggaacaattt cctgtactgc ttcatgcagg cgcagaatga cataacgcca
     6421 gggaacagta tgtttttctc gtttattgcg ttcgacttcc tggagatttt tttctgccca
     6481 gtcaaatggg tcgcgttgtt tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt
     6541 caggctctct aaggctatgc gttggctcca gcctggatca gcaaacttga tttcctctac
     6601 tatcaatcta aatacccgat ccaaaaggcc atattcgcct ttttgaatct cagcatttaa
     6661 tgcttgatca gtgacgatgt gtaagggctc gtagggaata gggcaataga aatcggcttc
     6721 gggtaaaata cccgtgcgcg acattaacgt aatatttagc ttttcacttg cgttatcgcg
     6781 gtgaaaaatg acgtgttgtt tatcatcttc aatgaacgaa ccatgctgaa tagccactgc
     6841 cattgccgca tccagtccgc tcaaggatgt tcccataata cccacgttac acgcatcgac
     6901 ctttgcttcc atcaggcctg accacgggct gggaaagtac gttctggttg cttcttcttc
     6961 atcaggccag acgtgacccg tggcaatcac cgctaaatca aacgtctcgc tgggtaaatc
     7021 ctgatttgta gcgagcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga
     7081 ttcataaaca gccaccgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg
     7141 atcgcggaaa tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc
     7201 ttttttaacg ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata
     7261 cgtacaataa atcggcggta tttcaatact ggcaatattt gccagcatca gttttgagtt
     7321 ttcctcatca ctgtatggca tcccgacacc ggcttcgtca gcctgctcga agatagaaat
     7381 agaaagtgga gtttgttgct gtagaagcga gaataaggtg tagatccccg taggcccggc
     7441 acccacaata gcaatttttt tcattgttac ttacctgtac gcattctttc gaatttagtg
     7501 ttatcagata agtttggttg aaatttttag aatttgagaa atttcagaca gggatgaaaa
     7561 gggcgctaac cgcccttggt taaaatctgt caggcgagtt ttgcctgatt gatttgactc
     7621 agtggctcat tgacatcgaa aactttggcg acaacttcct caacgccgag atttttcagt
     7681 cgggcagtat gtttttccag ataggcaagg gcgctttttt catcagtgaa aaggtagatc
     7741 ccaccggctt cgtggttctt ttcactttct gtccatacct tccacagaaa accaggttcc
     7801 tgattaatcg actcagcaag cggttcaagc tgctcagcca ttgcgtcgcc aaatgggcca
     7861 ttaaaagcaa aatgaagttg taacaaagtt gccatagcga ttctccttct gataacgcat
     7921 acgaagacag tgtaaatctt ttaatgtatt ggatacagag aagaaacaga gggaaaaagg
     7981 agaagaccag attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gccccccacc
     8041 atgtcaaggt ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga
     8101 ctcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc
     8161 aacgttgtag gtgacaacgg ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc
     8221 aaaacgtatt tttctgcggt atttcgaccg tttgcagagc ttttaagcaa attggctata
     8281 ttttgttgat ttgcaagggc gatttttatt caggatcgca tttacatctg acacaaccca
     8341 ataatctgtg tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga
     8401 taaatccttc gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga
     8461 ataaatcatg ggatctgatg cgaaaaactt gatgagcgac gggaacgtgc aaattgttaa
     8521 gaccggcgag gtcattggcg tgacgcaact tactgaaggt gagttaattg ttgaagctgg
     8581 cggaagagcc gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg
     8641 gatcgccaaa tgcacacagt acggcaacaa tggcacgcta tcggtcagcg acggtgccat
     8701 tgccacagat attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt
     8761 taatggccgc catcccgaag gtgaattcag cgttgataaa ggttatgcct gcggtttgtt
     8821 gctggaaaat ggcggtaacc tgcgtgtact ggaaggccat cgcgcggaaa aaattattct
     8881 cgatcaagag ggtggcctgt tggtcaatgg gacaacctca gcggtcgtgg tagatgaagg
     8941 tggtgaattg ttggtgtatc caggtgggga agccagcaat tgtgagatta atcagggcgg
     9001 cgtttttatg ctggcgggga aagccagtga tacattgctt gctggtggca ccatgaataa
     9061 tctcggtggt gaagactctg acactattgt tgagaatgga tccacttatc gtctggggac
     9121 ggatggtctt cagctctaca gttccggtaa gacgcaaaac ctgtccgtta atgtgggtgg
     9181 tcgggctgaa gtgcatgccg gtacgctgga aaatgcggta atacaaggtg ggacagtgat
     9241 cctgttgtca cccaccagcg cggacgaaaa ttttgtcgta gaggaagatc gcgtaccggt
     9301 tgaactgacc ggtagtgttg cattactgga cggcgcttca atgattattg gctatggcgc
     9361 agatctgcaa caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag
     9421 tacggtaaaa ggtgacagtg tcactttcag tgttggtaac atcaatctca atggcggaaa
     9481 actgtggctg atcactgatg cggcaacgca ggtgcatctg aaagtgaaac gcctgcgtgg
     9541 agagggagcc atttgcctgc aaaccagtgc gaaagaaatt tcacctgact tcatcaatgt
     9601 gaaaggggaa gttaccgggg atatacacgt tgagataaca gatgccagtc ggcaaactct
     9661 gtgtaacgca ctgaaactac agccagacga agacgggatt ggcgcaacgc tccagcctgc
     9721 gtaaaaacac aggcgcaacc cgtgaaggtt gcgccttgtc tttactagcg agctgctcgt
     9781 tgcagaatga tggctgacgg cagacgttgc aggaaccgca tacgcagcat catcataatg
     9841 gctgccgacg tcaggccaat aataaagcct atccagaagc ctgctggccc cataggttca
     9901 acgaccagat cggtcagtgc cagaatatag ccgcttggca agcccagcac ccagtaagcc
     9961 gtaaaggtaa tatagaaaat ggaacgcgta tctttatatc cacgcaaaat cccactgcca
    10021 atcacctgga ttgagtcaga aatctgatat accgccgcca gcaacatcaa atgcgcagcc
    10081 agcgttacaa cctcgggatt gtcgttgtac aacagggcga tttgctcccg cagtgaaacc
    10141 gtgaaaatgg ccgtcagggt tgccatacag acacccacca taagcccggt ccgcgcagcg
    10201 gtttgcgcat ccagcgttga gccctgaccc agacgataac ctacgcggat ggttaccgct
    10261 gccgccagcg acatgggaag tacgaacatc agtgaactaa agttcagggc aatctgatgt
    10321 cctgcgacat caacaatacc gagcggagac actaacagag ccacgacggc aaacagcgtt
    10381 acttcaaaga acagcgccag cgcaattggc aaaccgagtt gaatcagtcg tttcataacc
    10441 gcaggatcgg gcttcgcagt gcctttttcg ttacgaatat cgcgcatcga gcgggcgcgt
    10501 ttaatgtaag aaaccatggc aaggaacatg acccaataca ccgcagcagt agccacgcca
    10561 caaccaacgc caccgagctc aggcataccg aaatgaccgt aaataaagat atagttcacc
    10621 ggaatgttca ccagcaggcc gataaagccc atcaccatac ccggcttggt ttttgccaga
    10681 ccttcacact ggttacgggc aacctggaag aaaagatagc ccggcgcgcc ccatagcaac
    10741 gcacgcagat aacccacggc tttgtccgcc agagccggat cgatgttttc catggagcgg
    10801 ataatgtagc ctgcattcca cagcaccagc ataatgagaa cggaaacaaa acccgccagc
    10861 cagaaaccct gtcgcacctg atgcgcaatg cgctcacgtc gaccggaacc atttaattgc
    10921 gcgataaccg gcgttaatgc cagcagcagt ccgtgaccaa agaggatcgc cggaagccag
    10981 atagaagtac cgatagcgac cgccgccatg tcggtggcac tatagccgcc cgccatcacg
    11041 gtatcaacaa aacccatcgc agtttgggcg atttgcgcga gaatcaccgg gattgctaat
    11101 gctaataaca gacgcgcttc actgatatac ttctgcacgt gaacaccttt tatttgtagt
    11161 tatatgagag actaaaaagc cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc
    11221 agtctattgt agcgaggatt tattaattct ccagtgaaaa aatagccatt cttgcccctt
    11281 cactggtaag ggcgttttcc acctgctatt gtgctgaaca gaatgtctta actgatttca
    11341 ggagttgtaa gtatgtttac ggggattgta cagggcaccg caaaactggt gtcgattgac
    11401 gagaaaccaa attttcgcac gcatgtggtg gagttacccg accacatgct ggacggcctg
    11461 gaaaccggtg cgtccgtggc gcataacggt tgctgcctga ccgtaacgga aattaacggc
    11521 aaccatgtca gttttgacct catgaaagaa acgttacgca ttaccaatct tggcgattta
    11581 aaagtggggg attgggtaaa cgttgagcgt gcggcgaaat tcagcgatga aattggcgga
    11641 cacttaatgt caggtcatat tatgaccacc gctgaagtcg cgaaaatatt aacctcggaa
    11701 aataatcgcc agatctggtt taaagtccag gatagtcagt tgatgaaata tatcctgtac
    11761 aaaggattta ttggcatcga cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt
    11821 ttttgcgtcc atttaattcc ggaaacactg gaacgcacga ctcttgggaa gaaaaaactc
    11881 ggcgcacgcg tcaacattga aatcgatcca caaactcagg cagtggtaga tacggtagaa
    11941 cgagtgctgg cggcacgaga aaatgccatg aatcaaccag gtacagaagc ctgatctgaa
    12001 atcgcccccg gagagggggc gatagaatag cctttagcga gccactcgaa ggccgttttc
    12061 aacaccgcgt gagaacacga cctgccagag ctgaatatca cgggcgcgga aagcacctgc
    12121 acaggcattc agataatagg taaacatccg tttaaagcgt tcactgtagt tatccgcaat
    12181 ttctggccat gcggcgagga atcgttcata ccacgccatc aacgtagtat cgtaatcagc
    12241 accgaagtta tgccagtctt ccatcacaaa gtggggttcg ctggactgag caatctggcg
    12301 tacagagggc aggcaaccgt tcggaaaaat atatttatta atccagggat caacattcag
    12361 atcggttttt ttcgaaccga tagtatggag caggaatatg ccttccggtt tcaaattacg
    12421 atccaccacc gcaaaataag tatcgtaatt tttcggtccg acgtgctcga acatccccac
    12481 agaaacaata cgatcaaact ggtcgttcag gtcacgataa tcttgcagca aaatggtgac
    12541 atccaggcct tcgcagcgtt cctgagccat tttttgctgt tcggcagaaa tggtgacgcc
    12601 aaccacgctt acgtcataat tagatgccat gtagtgcgcc agtccgcccc agccgcagcc
    12661 aatatccagt acgcgcatcc ctggttttaa ctgcaatttt tcacaaatca ttttgagctt
    12721 cgcctgctgg gcagattcca gattatcggc atctttccag taagcgcagg aatattgcat
    12781 gaagggatca agcatgcggc tgaacaagtc attacccaaa tcgtaatgct ctttgccgac
    12841 tatccaggca cgttttttac tctgcagatt gaagagacga gcgccggcaa tacgcagcgt
    12901 gtctttgaaa tgatggggga gttggttctc gagacctgcg cgtaagactt tgctaaaaaa
    12961 catatccagt cggtcacatt cccaccagcc atccatataa ctttcgccta accccaacga
    13021 tccttcctgc agaacgcgtt taaaaaaatc ggggtttttc acatgaatat ccgccggggc
    13081 agaaccgtta atggctatac cggcacggct aagtaattcg ttggcgatac ggtaccagtt
    13141 gtcatccggt acactgactt cttctataca cgatgaactc atagtttctc catcactggt
    13201 cggtgatcag aaccttaaaa cagcgtagac gctttttggg ctttgtgaga aatctcgcag
    13261 aaaaaaccgc gagtctataa tccgacgtag aacagaggga aaggaggaac ccttgccgaa
    13321 atgaccatcc atagcgagtc gggaacgatc ctgtccccgt aaaaacataa tatttatcgt
    13381 agctttaaca actcgaaatt agtataggcc gctaatttca atcatacaac aaaattttgt
    13441 tagcagccta agtataagca tatcgatata gatcagtgtg attcgctatg agcgacttcg
    13501 gcattgccat gattctggca gtctacttct tcacaacgtt gcatcatgta acccagagca
    13561 accagcacta ctgttgataa catcacgctg gtggtggtga gcaatggcgt gctgatactg
    13621 ataagccagg aaactaccag acttgcgagg aagcacagac ccagttgtag agtgttctgc
    13681 aacgctgcgg cgcgaccagt tgcatgtggg aagggacgca gcgcctgggc gacaacaatc
    13741 gggtagatcg cgccattggc aatcgccatc acacagaatg ggatcaggat ttcgactagc
    13801 gacacatggc taatgaagcc cgcagcccag gtcgcaatga cgctgacagc aaacagtacc
    13861 agcaaccacg gtaataactg cttgccttgc catttctgca gtgcggcgcg acagccataa
    13921 ccaccaatca gaaacgcgat agtttgcggg acataactta aaccaataac tgccgggctg
    13981 tagcccattt cactaaggat gaacggtgag ccggtcagcc atgcaaaaaa actggctgaa
    14041 caggccgcat atatcagcac gttgccgcga taggttttag aacgtagcag gtcggtaaag
    14101 gtcagaccat cctgactatt gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc
    14161 agaatcagca ccacggtaat ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg
    14221 accagcagcc agcttcctaa cagaggagcc agtgccggag atagacccac cagcggcatg
    14281 atggtcgcaa aaatacggtt aactttctgt gaaggataat aatccgtcac taacgcttgc
    14341 cagataaccg ccgcggcgca gacacccaca gcctgtacaa aacgcaatac cagcagcgta
    14401 gcggcgtttt ctacccacag catccccaga ctacctaacg caaaaattgt caggccgatt
    14461 aataataccg gtttacgacc ataacggtcg gagagcggcc cccacagaag ctgggctgcg
    14521 gcaaaaccgg caaggaacag actaaggctg gcactgacag cagacgcagg cgtttgcagg
    14581 tcggcctgta tggcggcgaa agcaggcaga tacatatcgg ttgccagaaa accgagtacg
    14641 ctcaaacccg ccagccagac taaaaatctt ttcccaggtt gcatgtatat tctctaaatc
    14701 tctcaattaa acaccgccgc aaagtgtagg gagtgaaatt agtgttgtga aacggtaata
    14761 tttgatgact ggtttcaaaa attttgcagg cagactatgt ggtcagaata ttcactcgaa
    14821 gttgttgatg cggtagcgcg taatggtagt tttagcgctg cggcacagga gttgcatcgc
    14881 gttccttctg cggtcagcta taccgtgcgt cagctggaag agtggctggc ggtgccgctg
    14941 tttgaacgac gtcaccgtga tgtggaactg accgctgccg gggcgtggtt tctcaaagaa
    15001 gggcgctctg ttgtcaaaaa aatgcagatc acccggcagc aatgtcagca gatagcgaac
    15061 ggctggcgcg gtcagttagc tatcgcagtg gataatattg tcaggccgga acgtacacgg
    15121 cagatgatcg ttgattttta tcgccatttt gatgatgtcg aactccttgt ctttcaggaa
    15181 gtgttcaacg gtgtctggga tgcgctttcc gacgggcgcg tggaactggc gattggcgca
    15241 acacgcgcga ttccggtagg cggtcgttat gccttccgcg atatggggat gctaagctgg
    15301 agttgcgttg ttgccagtca ccacccgctg gcgttgatgg atggcccgtt cagcgatgat
    15361 acgttgcgca actggccgtc gttggtgcgc gaagacacct cgcgaacgct acctaaacgt
    15421 attacctggt tgctggataa ccaaaaaaga cttgtcgtgc ctgactggga atcatcggca
    15481 acctgtatct ctgcaggatt atgcataggg atggtgccaa cacatttcgc caaaccgtgg
    15541 cttaatgagg ggaagtgggt ggcactagaa ctggaaaacc ccttcccgga ttcggcatgt
    15601 tgtctgacat ggcagcaaaa tgatatgtcg cctgcgttaa cctggttgct tgagtatctc
    15661 ggcgatagtg agacgctgaa taaggagtgg ctgcgggagc cggaagagac tcccgcaacg
    15721 ggtgattaac gacgatagtc gcggaacggg ccgtcagcca cggagcggcg ttcaatcaac
    15781 cgcggatgca cttcaatgga ttgcggttct tcgcgtttat tgacgatacg atccaacagc
    15841 atattgaacg cagtttcgcc cagcgaatct tttggctgat ggatcgtggt cagcgccggc
    15901 gtaaaatagc gggcgttgcg cacgttatca tacccgatca gcgaaacatc ctgcgggacg
    15961 cgcagaccca tttcatcagc agcacaaagt gcgcccattg ccatgatatc gccaccacag
    16021 aagacggcag tagggcgatg cggctgcgac aggatttgct gcatggcgcg ataaccggat
    16081 tcaggttcaa agtcaccctg cacaatccag ctttccggca ctttgatcat cgcttcttcc
    16141 atcgccttca taaaaccggc aaggcggcct gcgccggtgt tacgttccag tgggccaggg
    16201 ataacgccga tttcgcggtg accgcgttca atcagataac gcccggccat gtagccgcct
    16261 tcgaacgcgt tatcaatgac cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc
    16321 accaccatcg ggatatggcg atactcttcc agcatcgcca gcaacggctc tgggtactca
    16381 gaacacatca ccagcagacc atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc
    16441 tgtttctcaa gattgttcca cgcattgccc agaatcaggg tgtaaccttt ctggaagcaa
    16501 tttttttcaa ccgcttcaat gatctcggca aaataggccg cttcgctgct ggtcgccagc
    16561 aaaccgatag acttggtgtg gttaaccttc agactacgcg ccaccgcgct aggggagtag
    16621 tgtaattctt tgatcgctgc ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt
    16681 ttgttgatca cgtgtgacac agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt
    16741 attgttgcca ttccatttca ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta
    16801 aacgtttgcc tttacacacc ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga
    16861 cacggagcgt caggaagggg tcaatggcca gtacgcaaat agtgtaagcg tggaattttg
    16921 gccgatcttg acgaaaaggg gaagtggtaa aaacacttat cattataaat gacgggagat
    16981 tttttcatca cagtgtgtaa aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga
    17041 gaaaacttga tgagcaccga ccttaaattt tcactggtaa caacgattat cgtcctcggt
    17101 ttgatcgtgg ccgtgggttt gactgccgcg ctgcactgat ttcctctcga gggagcgcgt
    17161 tctccctcgc atctccttcc tgtttgttag caaaatggca aaagtgtttt ctctttttgt
    17221 tattttcatt tttttgtgat tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat
    17281 gacgcgaaga ggtggagata aggcatgaaa attaactatc cgttgctggc gctggcgatt
    17341 ggcgcgtttg gtatcgggac aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg
    17401 cgcggtgtgg atgtctcgat tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc
    17461 gtaatggttg gcgcgccgct gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg
    17521 ttgattttcc tgatggcgat tttcacgctc ggcaatgtac tttccgccat cgcgccggat
    17581 tatatgactc tgatgctttc acgcattttg accagcctga atcacggagc attttttggt
    17641 ttgggttcag ttgtggccgc aagcgtggtg ccaaaacata aacaggccag cgcagttgcc
    17701 actatgttta tggggttaac cctggcaaat atcggtggcg tgccggcggc gacctggttg
    17761 ggtgaaacca tcggctggcg gatgtcattt ctggcaacgg cggggctggg agtgatttca
    17821 atggtaagtc tgttcttctc attacctaaa ggtggcgcag gggcgcgacc tgaagtgaaa
    17881 aaagagctgg cggtattaat gcgtccgcag gtgctgtctg cattgctgac gacggtactg
    17941 ggagctggtg caatgtttac cctctacacc tatatctctc cggtactgca aagtattacc
    18001 cacgcaacgc cggtgttcgt tacggcaatg ctggtgctga ttggtgtcgg attctctatt
    18061 ggtaactatc tcggcggcaa actggcagat cgttcagtta acggcacgtt gaaaggcttt
    18121 ttgttgctgc tgatggtgat tatgctggca atcccgttcc tggcccgcaa tgagttcggc
    18181 gcagctatta gcatggtggt gtggggcgct gcaacctttg cggtcgtacc gccgttacag
    18241 atgcgcgtga tgcgtgtcgc cagtgaagcg ccaggtctgt cttcatcagt caatattggt
    18301 gcctttaatc ttggaaatgc gctgggagca gctgctggtg gtgcggtaat ttccgctggg
    18361 ctgggataca gttttgtgcc ggtgatgggg gcgatagtcg cgggactggc attattgctg
    18421 gtgtttatgt cagccagaaa acaacctgag gcagtttgcg ttgccaacag ctaattaaac
    18481 aaaaacgcag aaggataacc ttctgcgtta cagttgctgg caacgtgcga tttatcttgc
    18541 cggatgcggt gttaacgctt catccgagtt ggcatggtaa gcgaagcgca tcaggcaatg
    18601 ttgcatttgc catcagttat tatgcagcga ggttttttgc tacgaattcc cagttcacca
    18661 gcgcccagaa gtgctccaga tagccaggac gtgcattgcg atagtcgatg taataagcgt
    18721 gttcccagac atcaacggtc agcagtggag tcgcatcggt ggtcagcgga gtacccgcgt
    18781 tagaggttga aacgatagcc agtttgccat cgctgttttt caccagccag gtccagccag
    18841 aaccaaagtt tttgattgct gcatcagtaa actgtgcttt gaaatcggca aagctgccaa
    18901 aagatgcggc gatagcttca gcgacttttc cagtcggttc gccacccgcg ttcggtgcca
    18961 ggcagttcca gtagaaagta tggttccaga cctgagctgc gttgttgaat acgccacctt
    19021 cagagctgcg aataatctct tccagtgatt taccttcaaa cgcggtacct ttaatcaggt
    19081 tgttcaggtt agtgacataa gtctgatgat gcttgccgta gtgatactcg atagtttccg
    19141 cagaaatgtg cggtgccaga gcatctttag catatggtag tgcaggtaat tcgaatgaca
    19201 ttgctactct cctttattat taatttgcat acgtacaata gccttattgt gcgtatggat
    19261 agggtagcaa attaaaaggt gagacaaaag atgaacttac cctgttgccg taacaacagg
    19321 gtaaagtttt agcgaagtgt ttttggagtc attacccggc gagcgccaac atagtggcgc
    19381 tgccagtagt cttcactgag agaagtgatt tgaatttcct gacccgtacg cggtgactga
    19441 ataaatttgc cgttgccgac atacacgccg acatgatcgg ctgtaccgcg tccctgagta
    19501 cggaagaaga ccaggtcgcc gtttttcagc tcactacgtt cgataggtgc tgcatcacgc
    19561 aggtgataca tttcattcgc cgtacgcgga atacgaattt tcaccaaatc tttataagcg
    19621 taataaacca ggccgctgca atcaaaaccg gtacgcggtg agctgccacc ccaacgatat
    19681 ggcttaccaa tttgctgcat cagtttattc attgccactt ttgtggcttt ttgcaccttc
    19741 gctttgtgtg cgtcggcaag ggttcctgac gcggcatttt tgactttcac acaatgcgat
    19801 ttatagccct tacgtttggt acacttttcg gtaaagctgg ctgaggccgt tttattgacg
    19861 gcgtgtttgc tgcgagtttt ggcgttttta gaagcggtaa tggaagattt tttaacggaa
    19921 gaagcggttt tgcttttggt cgtactttta ctggcggctt ttttcgctgt tttctggctg
    19981 gctttctggg tctttttggt ggttgccgtg cttttctttt tatctgcttt tttggtgata
    20041 tgagtggtag cagaactctc cctggcttgc tttgaagcat gggccatagg tgttaaaggc
    20101 agggtggtaa aaagtaaagc acagagcgtg atcgaaatac ggtttatccg cgccactgag
    20161 cgtccctctg ttaaggcagg tctatcatgc ctgcgtctga tttacaaaac ttgatgattc
    20221 taatgcatag aagaagagaa agttaatagc ttttattgtt aaaaaatacg tttcgtcggc
    20281 gagcgcaaaa aaatgcagca gtgaaagcta aaaatggtta taaatacgcc agtttgccgt
    20341 gcataaggga agaaacgaat atttgaaatg caacaaatag agcaaaggga taaaatagca
    20401 aaagcgtgat gaaaatgtta ttttccgatg ccgcactgac ccgctacaat gtcagattac
    20461 tgccgtaaag aagtttgagg aagcaagaca atgagcacca ctatcgaaaa aatccaacgc
    20521 cagattgctg aaaacccgat cctgctgtac atgaaaggtt caccgaaact gccgagctgc
    20581 ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt
    20641 gatattctgc agaatccgga cattcgtgcg gaactgccga aatatgctaa ctggccgact
    20701 ttcccgcaac tgtgggttga cggcgagctg gtcggcggtt gtgatatcgt gatcgaaatg
    20761 tatcagcgtg gcgaactgca gcagctgatc aaagaaactg ccgctaaata caagtctgaa
    20821 gagccggacg cggaataatc cgtcctgcag tactgacgga tgcgaacggg catccgtcag
    20881 ttctttatct atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc
    20941 agcgtaaaca tcggcgtttg ccggaccggt agatcattca cttctgttag cgtaaagcgc
    21001 agccgtggtt cgcgacgcag tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat
    21061 tcatcttttt cctgccagac cagcattttt ttgccgcctt gcgccagata gagtaacaat
    21121 ttgccgccag aaacgaccac cagcgcaccc gcccgacgcg ttggaaccag cgttgccgga
    21181 tgtgcaggcc agggcagaag atttccccac acattagccg gatcgttggc ggagagcgcc
    21241 actggtgtat agtggcgcgt ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata
    21301 gtcagacgtt cagcgaattg cgcacccccc agaccttcta caaaacgacc tcgcataatt
    21361 cgcccggagt cttccatact tcgacaaagc gtttgcatcg atggaaaccc gccagggata
    21421 ttttcggcta tcaccgcctg acgactgatg atgccgtagc ggtcgagcat attttccgcc
    21481 agcgccagca tcctttcggt atcgtttagt ggctccacct gcaataacga ccagcgtcca
    21541 gcaagatttg gtgtgttgta agatacccgt ggcgagacgg gttgtgcata gacaggacgc
    21601 ccacgacgag cccggtgact gcggcgagtc gaggtgcgtg cgttggaact gctgcgggtg
    21661 agggcgcgta acggtgccca aatgtcgctg gtgatgacgc cttgccagac cagcgcccat
    21721 aacgcctctt gcatggctga gggatcaacc gattcgccga ttttatcgcg tatccgttgg
    21781 ctgatttgtt gtgcaaacca ggctcctccg tcagccagaa cagcgattat cgcttgctgc
    21841 agcgccgaac gattcgcctg atctgcttcg gcgggagtga acgattctgc ggcatattcc
    21901 tgtagatgca gtgccaccag accgtcatct tcacccagct ttttttgccc cgaccagata
    21961 accgcaccgg ttgccagtaa ttcatcgagc atttctggcg aatagtcgcg tacgcgggca
    22021 ggcaggatct ggctttccca gagtgaggcg ggtaaaccaa cgccagcaag ctgttcgatc
    22081 acccgcatca cgccatccac gccctcataa acgcctggcg atgttgaggc aaagagtgcc
    22141 gggctaccat cggtggcggg taatacgccc tgacgttcca gcagcaatcg cgcataggtc
    22201 gtggccgcaa cgggacgtgt cgcttctctg gcggcttgca gcgagcgcaa acgcagacga
    22261 cgaaatactt catcgctcac ccagatgtct tgttgcagat tcatcaccag cccctgttcg
    22321 cgcagttgct gaagttgctc ttcgacaatg gcaataccga gactaaactc atgagccagt
    22381 tgttcagacg tgaccagagc atgagcccgg agatagcgca gaaagaggtc gcgaagcggg
    22441 taactgactc ggtgcaaata aatctctggc agtgactctg gtagtcgtac gccgagagca
    22501 tcgcgcagcc tggcggcatc atccatacag gcaagacgct cctgcccgct aatcatggtc
    22561 gggaagattc gttttactgc aagaagattt tccagatacg acgcaatctc ttcactgctg
    22621 cctgtatgtc gttgcgccag gtcttcaacg gtcattggcc ccagttcgcg cagcaggtcg
    22681 aacaatcctt cttcaccttt cgctcttctg ccaggagcca gtcgttgcaa ctcttcttcc
    22741 acctggcgaa tgacctgcgg gtcgagtaat tcccccggat cgacctgtcc gagtagattg
    22801 cgcagtaact cgctgtccag cgacagtacg gatgcccggc gctctgccag cggggcgtcg
    22861 ctctggtaca taaattccgc gacatagccg aacaataaac tggcggcgaa aggcgaaggc
    22921 gtagtggtcg ttacatcgga tatttgaatt tcgccaccgt tcaggcgacg catcaaacgt
    22981 tccagcgcgg gaagatcata aacatcttgc agacattcgc gtagtgtttc gagaatgacc
    23041 ggaaaatccg gatatccctg agcgatttcc agcaactgac tggcgcgcag ccgttgttgc
    23101 cagagcgggg tgcgatggcc cggcgtgcgg cccggcatta ataatgcccg cgcggagcat
    23161 tcgcgaaaac gggcggcgaa aagtgccgag ctgcctaccg cctcgcggac aatttgcagc
    23221 aacttttctg gttcaaacaa aaaaatcgcg gcatcaggca atttaccatc ggtgtcagga
    23281 atgcgcgcaa caatgccgtc atcactggca accacagacg cgtcagcgcc ccatagtgca
    23341 tgtattcgcc cggcaatcgc cagcgcccag ggttcatgca cccgtcttcc atagggagag
    23401 tgcaaaataa tacgccagtc accaatttca tcacggcacc gttcgaggac cagatgacga
    23461 ctacctggaa cgatgcccgt cgcgttgcgc tgctcgtcaa tcaacccctg aatattggcg
    23521 atcgtatttt cttctgccaa ccacggggga atagtcccgg aaaagaacgc gccatccgcc
    23581 agtaaatgga gaaaatcgcc gatcatctcg cctaattctg ccggacgtcc gttaccttca
    23641 ccacgccaga agggaaggcg ggcagaacga cctggagcag gagtcacaat cacctgatcg
    23701 cgggtgattt gctggatccg ccatgaggta gcgccgagag tgataatgtc gttcacccgc
    23761 gactcatata ccatctcctc atccagttca cccacccgcc gcgaaccggc ctgttcttca
    23821 ccttcgggta ataacacgct atacatgcca cgatccggaa tggtgccgcc gctggtaacc
    23881 gccaacaatt gagcgccagg tcgggcggtc aatatcccgg tctcccggtt ccagaccagt
    23941 ttggggcgaa aagcagaaaa atcgccagag ggatagcgcc cggaaagcat atccagcgtg
    24001 gcgtcaaaaa cacgtcttgg cagatctttc cacggtgcgg cacggcgtac gcgggagtac
    24061 cattcgtcta cctgtaatgc atccatcgcc gcggcggcaa cggtttgctg cgcaaggacg
    24121 tcgagtggat tatgcggcgg tgtcaggttt tccagcctgc ctgcgaacat acactctaca
    24181 atgactgcgg aatcgactaa gtcacgcctg gttcggggga aaaaaagccc tttggagata
    24241 ccacctacct ggtgtccggc acgtccaatg cgttgtaacc cactggcaac agaaagcggc
    24301 gttgccacct gaatcaccag atccaccgcg cccatatcaa tccccagttc gagactggag
    24361 gttgcgacca cgcagcgtaa ttcacccgat ttcagcgcct gttcggtgat tgctcgttgt
    24421 tctttagaga cggagccgtg gtgtgagcgg gcaataaaaa tgtcgctatt ttgtacacgg
    24481 ttagaggttg cgccggaggt cgactcgaaa tggaccgcat caacggcgat agacggggaa
    24541 cgctgtaagc gtgcggcgta aagctcattc aatcgtgccg tcagtttttc cgccagcccg
    24601 cgtgaattag taaagacaat ggtcgagcga tggcgcaaaa cttcatcaag gatacccgtt
    24661 tcaatatatg gccagatgga gccttcccgg ccggcatggc tgtcttcgcc ggtgccgctg
    24721 gcgaccgatg agacatcatc catattggcg accggtacga caatccgtat ctgcggatgg
    24781 cgcattgcgg gcgggttgac taccgtaacc gggcgatcgc cgccaagaaa tgctgccaca
    24841 tcgctggctg aacgcacagt ggcagaaagg ccaattcgct gtgctgaggt gtggagctgc
    24901 gcatcgagcc gctccagact taacgccaga tgcgcaccac gtttactgcc tgctaccgcg
    24961 tggacttcat caataattac cgtttcgacg ccgcgtagcg tttcgcgcgc gcgggaggtc
    25021 agcatcagat agagtgattc gggtgtggtg atcagaatat ccggcggatt acgggtgagt
    25081 ttgctgcgtt cctgtgcagg cgtatcgcca gtacggatcc ccacgcgaag attgacttcc
    25141 gtttcgccgc gccgccgccg ttcatcggca ataccctgta acgggatctg caagttgcgc
    25201 tgaacgtcgg tgcccagagc tttgatcggt gaaatataga ggatgcgtga ggttttacgc
    25261 ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga gctgatcgag ggcgtagaga
    25321 aatgctgcca gcgttttccc ggagccagtc ggtgcaatca ccagcgcatg ttcgcctcgc
    25381 gccgccacat gccaggtttg cgactggaca gcggtcggct gtttaaaggc gcgaagaaac
    25441 cagtcgcggg tcgccggtga aaacacgtcc ggcaggggcg atgaagggtt tagattatct
    25501 gccattaacg ctattccccg tgaatatcat gcatgatgcg tggaatagcc tcactataga
    25561 tgcctgaatc attgcatgtc atcaggcatc gactcgatta cacctcttcg gcggcaggta
    25621 gcggccagcc tcccagacgt ttccagcggt tgacgatttc acaaaacagc acagcagtgc
    25681 gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc gaagtccatg ccagcggtct
    25741 ggcaagcctt tgacaatacg gtttgtccga gtgccagccc ggcaagtgca gcagtgtcaa
    25801 aagtggcgaa agggtggaac gggttacgtt tcagtgaggc gcgttctgcg gcggccatca
    25861 taaagctgtg atcgaaattg gcgttgtgcg ccaccataat ggcgcggtta cagccgctcg
    25921 ctttaatgcc tttacgtaca actttaaaaa tttcgtgcag cgcctcgtat tcgctgaccg
    25981 cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc gagggcctct ggctgcaaat
    26041 ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt gtccggcatc agccagcctt
    26101 gttcatccat tttcagggtg atggcggcaa tctcaagcag cgcatcggtt ttggcgttaa
    26161 atccggctgt ttcaacatcg atcacaacag gataaaaacc acgaaaacgg tcgcacagac
    26221 cggtaagttg agcgttatcg gacatctgat tctcttaata cggataaatt gcagcgcgca
    26281 ttatgacaaa tattacttgt cgatgcagta aagatgcggg cgcgaggagt tcacgcccgg
    26341 caggagatta gttgcccaga ccgcgaccgg cgtctttctc ttcgattaac tcaattttgt
    26401 aaccgtccgg atcttccaca aacgcgataa ccgtagtacc gccttttacc ggacccgctt
    26461 cacgggtgac gttacccccg ttttgacgga ttttttcgca cgcttcagcg gcgttatcaa
    26521 cgctaagcgc gatgtgacca taagcagtgc cgagttcgta tttatccacg ccccagttgt
    26581 aggtcagttc aatcaccgct tcttcggttt ccgggccgta gccaacaaac gccagtgagt
    26641 atttgtattc cgggttttcg ctggtacgca gcagtttcat gcccagcact ttggtataaa
    26701 aatcgatgga gcgttgcaaa tcgccaacgc gcagcatggt atgaagaaga cgcatcttta
    26761 tatcctcaaa atggctaaca gattcaaaat gttgttttag tatagcggcg gctttgcgcc
    26821 gctcgcaatg ttggattaca gcgtcgggta atccgtgtag ccttccgcac cgccgccata
    26881 gaaactttca gcacgctgtg gattaagctc agctttgcgc tgcaagcggg cgaccagatc
    26941 cgggttcgca atccagtcac gaccaaatgc caccgcatca attaaccctt tgccaatcag
    27001 cgtttcggct ttttctaccg tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc
    27061 gcgtactttt tcgcggaacg catcagtata cggttcacct cccgcccaat ctggttctga
    27121 catatgcaga taagcaatgc cgcgtttacc cagttgttca atcagataca gtgcatcggc
    27181 ttcttcattc gggccgttat ccgtgttctg gaaagtaccg attggcgaaa cgcgaatgcc
    27241 aatgcgatcg gccccccatt cttcaatccc tgcatcgacc acttccagca ccagacgtgc
    27301 gcgattttcc acgctgccgc cgtactgatc ggtacgatgg tttgaagaag gagaaaggaa
    27361 ctgatgcagc aaataaccat gagcagagtg gagctctacc agatcaaaac cggcttcacg
    27421 cgcgttagca atggcctgac ggaaatcatt gacgataccc ggaatctctt ccagttcaag
    27481 cgcacgcggc atggatgttt caacacggat cgcctgacca ttttcatcgc gcagagaagt
    27541 acgtgttccc gcgctaagtg cggaaggcgc taccggtgcc tgaccgccag gttgcaggct
    27601 ggcgtgagaa atgcgtccgg tgtgccacag ctgtacggcc atatgaccat tttcagcatg
    27661 aacgccagcg gtaatttttt tccatgcggc aatttgttcc ggactatgga tgccaggtgc
    27721 accagcatac ccttttgcct gggcagaaat ttgcgtggct tcgctaataa tcaaaccggc
    27781 actggcacgt tggcgatagt attccgccat caacggggta gggatgtcac ccggttcaat
    27841 actgcgcaga cgcgtcagcg gtgccataaa aatacggttt gccgccgtga tcgcgcccac
    27901 tttcagtggg gaatacagtt tttcagatga cataaaggct cccggtcgtc tggtgataaa
    27961 aaatgcctgc taaacggcag gcgtcgcaat aatggttttt acatgtgcca gcgcgttttc
    28021 cagtggctcg gaattgcgcg aaattttggc ctgcagattc gcacccagcc atagtgcgta
    28081 aagcacttgt gcctgttgca gcggttcgcc acaaaaggtt aaacaatggc tatcacggcc
    28141 attttccagc gcctgcgaga gcagaacgat cacgccgcga gcgcctttat ccatcgcgct
    28201 acgcatatct tctgacagat cgcacacttc ggcagagagt tttactgtca ggcaaccact
    28261 gatggttcca tgttggcaaa actggttcag tgtttgctgg taataagcca gtatgcggtc
    28321 gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt cgctggtgat atgcggcgta
    28381 atggcgctca agcatggcaa cgccaaacgc ttctttagag cgaaagtagt gatagaagga
    28441 ccctttcggc acttcagcag tttttagtaa ttcgcttagc cccatcccgg tgaatccacg
    28501 ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg cgagtatcat gttcggtgtg
    28561 tttgttcatg cgttggagtg tagtagacca gtcggtctag tgcaagcaaa gttgctactt
    28621 tgggcattaa cagcttcaat aatggtaata atcttatttg aggagggcga agtggcggag
    28681 caattagagt tttttcccgt ccagagcccg tgccggggaa tttgccagtc tgacgaacgc
    28741 gggttttgtc gcggctgttt tcgtagtcgg gatgagcgtt ttaactggaa taaaatgagc
    28801 gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc gattaatgcg caaattacgc
    28861 gcaaataagc cagcgtcatc agacgaaccg gagcaaccat cactcttttg agcctaatct
    28921 tgcgtatact cctctcatat ctgataagag gaagcgatta tggttcagcg tattactatt
    28981 gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct actggcgatt gatggactgg
    29041 aatatgtccg cccgccagct ggtcagtttt attgaagagc atctggatct cggcgtgacc
    29101 accgtggacc atgctgatat ttatggtggc tatcagtgcg aagcggcgtt tggcgaggca
    29161 ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg tcagtaaatg cggtatcgcg
    29221 acgaccgcgc gtgaagaaaa cgtcattggt cattacatca ctgaccgcga tcacatcatt
    29281 aagagcgccg aacagtcgct aattaatctc gcgaccgatc atctggatct gctgttaatc
    29341 caccgaccag acccgttaat ggatgccgat gaagtggcgg acgcgtttaa acatctgcat
    29401 cagagcggca aagtgcgtca ttttggcgta tcgaacttta cgcctgcgca atttgcgctt
    29461 ttgcaatcac gcctgccgtt tacccttgcc actaatcagg tggaaatatc cccggtacat
    29521 cagccgttac tgttggatgg cacgctcgac caactgcaac aactgcgtgt tcgtccgatg
    29581 gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg attatttcca gccgctgcgt
    29641 gatgaactgg ctgtggtggc ggaggagtta aacgcgggct cgattgaaca ggtggtttac
    29701 gcctgggtat tacgtttacc gtcgcagccg ctgccaatta tcggctcagg taaaattgag
    29761 cgcgtacggg cagctgtcga agctgaaaca ctgaaaatga cccgtcaaca atggttccgt
    29821 atccgtaaag cggcattggg ttacgacgta ccgtaagcac ttttaggaat agccgccgtt
    29881 caaaaatgtg tcactggttt acacttattc aggaatgcac aatgaacgga ggtcctatga
    29941 aacgttttag tctggccatt ctggcgctgg ttgttgcgac cggcgcacaa gctgccagtg
    30001 aaaaagtcga gatgaacctc gtcacgtcgc aaggggttgg gcagtcaatt ggtagcgtca
    30061 ccattactga aaccgataaa ggtctggagt tttcgcccga tctgaaagca ttaccccccg
    30121 gtgaacatgg cttccatatt catgccaaag gaagctgcca gccagccacc aaagatggca
    30181 aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg
    30241 aagggccaga aggtgccggg catttaggcg atctgcctgc actggtcgtc aataatgacg
    30301 gcaaagctac cgatgccgtc atcgcacctc gtctgaaatc actggatgaa gtcaaagaca
    30361 aagcactgat ggtccatgtt ggcggcgata atatgtccga tcaacctaaa ccgctgggcg
    30421 gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt aagaggccag cgtaccttgc
    30481 ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc gccagacaat tgccgccagc
    30541 tcacgggcgg caggttgatg atggcgggca aggctgtcgc aaatccgctg taattcttca
    30601 agtgtggccg ccagtgattt ttgctgaacg ccacgctcac tcatcactcc gcgcaacagt
    30661 gaaatacagt tatcccgtac tcgtgataac ggatcggaac gcgattccca gtcgcgtaat
    30721 tgccagacaa catgagaaca gttcagcagc actacacccc agcgtaataa ccaacggcga
    30781 gccagcgcat cctggctgtt actcaactga ctgacgtgat gataagtgag cgattcaaat
    30841 tcgctttcac tcagtgttgg atggcggctt agctgatcga caaaatcccg gcgcagcgcg
    30901 cgaatatgac gacggctttt acgagcaccc gaccctggac gcaaaatggc gaacgctaac
    30961 cacgccagcg caacgccaac gatttttgcc agattatcgt taagaaaatc agcaaaatca
    31021 tacaccgggg gattagtgac agcgataaac gaacccataa aaacaatcag ttgcccccac
    31081 agtgcggcaa atttgggcat ctgcaattta agaagctgca ttgtcgccag cagtggaaag
    31141 agaaacagta aaaattgcca cagatcgcta atctggacca tcaggccaaa tttgaccaca
    31201 aagctgaata gcgaaagtaa caccagagtg cgcatcagta gtgacaacga cttaaacggt
    31261 gctgcgacgg cggagtagag tacgcagcta attgctgcca gcgttaatgc attggcaccg
    31321 gcatcccatt gcgaagcaat actccatgcg ccaatcatca tcaacgtaca aaatgtacgc
    31381 agtccgctcc acatagcttc ggcgttatcg gtatgacgag ccaggccgga tgtccgtggg
    31441 agtctggtat gatcatccac accgctttgc agacgatgta attcctgact actttgcaga
    31501 taaaggcggc aaaaataacg tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg
    31561 ttggtcgggc gtagcggggc gataatacgt gcgacggtgt aaacatctgt ttgcgaactg
    31621 gcgagcgccg tcagcagctg ttcgagaatt tctcgtgtgg cacatggcgg tgagggccag
    31681 ttgagcaaca tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagc
    31741 agcgcattaa ggcgtgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata
    31801 cggagcaaat tcatggtcag tatctgtcca atcaccccct catgtgctgc acgaatggca
    31861 tcggttgttt caggctgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt
    31921 ttcaatgcgg ttaaaagggc agtagcatcg gaactgctcg gcaggatcat catcatcatg
    31981 ccgccgcaca aaataccgac aatcacctcg caaacgcgcg cctgagcgat atcccacagc
    32041 tggctggcct cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc
    32101 agttgaaatg catacgcgac attattcgtg aagtgcgcac aggcccaggt acaaaagcca
    32161 agccacgccg ccatgctcaa aagaaaaaac cacggctcat tgagcgtatg tccggcaaga
    32221 agcagtgccg caatggctcc gagcaaactg ccagcgatgc gtccgaggct tttgctgatg
    32281 acaccgccaa cggtgggaaa gctaaccacg gcagccgagg tcatcgccca atagggttca
    32341 tccagattta aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac
    32401 gcataacgcc attgcgccag cgtggccctg aaccagggca aattgcgcaa ggaccatgac
    32461 gatgcgttca ttattgacct acggcaatgc tacaggtggt gcccgacacc agcgtgacat
    32521 cgcctgggac tttatccagc gcaaagcgga cgggaacgcg ttgggcaagt cgaacccagg
    32581 ggacgttagg tttcacatcc ggaataaggc ttgagtcact ttcaacgctc tgatcataaa
    32641 tcgcccgacc gatactggaa acgtgaccct gtaacgtttt gttatcacta tagagggtaa
    32701 tttgtgctgg tgcaccttcg cggatatggc gcaacttggt ttcttcaaaa taaccaatga
    32761 cgtaaaacga gtggctgtcg acaagggcaa acagcggttt cccggtgtca gcgtaatcgc
    32821 cgatgcgggt agtgaggtta gtcacccatc cactcaccgg ggcgcgaatt tccgtttgcg
    32881 ccagttgcca ttgtgcctgt ttcagcgtcg cctgtgcggc atcaacactg gcctgcatcg
    32941 ctttaacatt gaggttagcg gtatcgagtt cttcggcaga aataaaattt tgtgagagat
    33001 gacgacgacg attcgcttcg ttattggctt ttgcgaggtc agattgcgct ttcgccagct
    33061 gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt atcgatcgtg agcagaagat
    33121 cccccgcgtt aaccagttgg ttgtctttta tattcagctc aacaatacgt cccgacacct
    33181 gaggggtgat agaaacctgt tcagcgcgta ttttcccgtc gcgtgtccac ggagattgca
    33241 tgtaataatt ccacagccac catccggcaa gaaccgcaac taccgctaca atgattgttg
    33301 agaaatactt aattgtttta attgacatag ttaccacgca atcagtattg ccagggcaag
    33361 gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag ggatgccaga tgtcaccggc
    33421 gtagatccag ccacgaagca ggcggtgtac gacaagccag atgacgaatc caaacgcgaa
    33481 ggctttgaaa aacggaggaa agtagacgga cgcaccgaac accagatctt gtaagggcaa
    33541 tcctgttgca ttgagcataa acttcacaaa gtagattcct ttacgaccga atatattgcg
    33601 ttagattaat aaatattctt taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt
    33661 ctgacggtaa ccaaatgcag caatacattt gttttagcaa tacaattgct gcacactatt
    33721 ctaaaagccg cataatatct tagcaagcta attataagga gatgaaattg gaatcgccac
    33781 taggttctga tctggcacgg ttggtgcgca tatggcgtgc tctgatagac catcgcctga
    33841 aaccgctgga gttaacacaa acccattggg ttacgttaca caatatccat cagttacctc
    33901 cagaccagtc gcaaattcaa ctggcaaaag cgattggcat cgagcagcca tcactggtcc
    33961 gtactctgga ccaactggaa gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc
    34021 gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc gctgatcagc gaaatggaag
    34081 ctgttattaa caaaacccgc gcggaaattt tacatggcat ctccgcagag gaactggaac
    34141 aactgattaa gctcatcgca aaacttgagc ataatatcat tgagttacag gccaaagggt
    34201 gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc aaacttattt agcgcgggga
    34261 aacggtcacc tgactgccat tgctggccag tacgacacgt tggcccggag agaaacgagt
    34321 gttgccttgt ttctgtacca ccatgatggt attaccatcg tctttacgaa tttccagctc
    34381 gacaccctgc gttttgttca ttgcactctg tacgccctga ccggctacgc cacctgcaac
    34441 agcacctgct gcagtagcca gagaacgccc ggttccgcca ccaacagtat tccccaggaa
    34501 accaccaaga acagcaccgc caattgcgcc gataacgttg gaatcatcac cgccctgaat
    34561 ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca ttctgtactt gtttcgcttc
    34621 agaagcggta taaacatccc ctgacagggt gtcgttatta acacaaccga caagagacag
    34681 acctaccatt gaaacaacca atacgcgttt aatcattgaa aaatctcctg ttcaccatga
    34741 aacgccacgc aagcatccct catggtcaaa gtatatggca tatgtgaatc catggttaca
    34801 tgttctacca aatatgagca aatcataatg aaagtaggat caaccaatta taaacaaaat
    34861 attcatgatg attattgatt tgaagataat gttttactac caacggtaaa aatcggtcat
    34921 tccacatagt attcacgaaa agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa
    34981 ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt caggcaccag ccttgatggt
    35041 gttgatgttg tgttggcgac aattgatgaa caccgggtcg cacagctggc aagtttgagc
    35101 tggccgatcc cggtgtctct gaaacaggct gtactggata tttgccaggg ccagcagctt
    35161 acactttcgc agtttggaca gcttgatact caactcgggc gactttttgc tgatgcggtc
    35221 aatgccttgc ttaaggaaca aaacctgcag gcgagagata tcgttgcgat cggctgtcac
    35281 ggtcaaaccg tctggcatga accgacgggc gtggcaccac acactttaca gattggcgat
    35341 aacaatcaaa ttgtggcacg caccggaatt acggttgtcg gtgattttcg ccgtcgcgat
    35401 attgccttgg gaggacaagg cgcaccgctg gtacctgcgt tccatcatgc gctgctggct
    35461 cacccaaccg agcgacgaat ggtgctcaat attggcggca tcgcaaatct gtcactgctc
    35521 attcctgggc agccggttgg gggctacgat accggtcctg gtaacatgct gatggatgcc
    35581 tggatctggc gtcaggcagg taaaccttac gataaagatg ccgagtgggc acgggcgggt
    35641 aaagtcattc tcccactgct gcaaaatatg cttagcgacc cgtatttctc gcaacctgca
    35701 ccgaaaagta ccggacgcga atactttaac tacggctggc tggagcgcca tttgcgccat
    35761 tttccgggtg ttgatccccg agatgtacag gcgacactgg cagaactcac cgccgtgacc
    35821 atttctgaac aagttttgtt gagcggtggc tgcgaacgat tgatggtatg tggtggagga
    35881 agtcgtaatc cgctactcat ggcgcgtctg gcggcattac tgccaggcac agaagtcacc
    35941 accaccgatg ccgttggcat tagtggcgat gacatggaag cattggcttt cgcctggctt
    36001 gcctggcgga cgctggcggg attaccagga aatctgcctt ccgtcactgg cgcaagccag
    36061 gagacggtac tcggggctat tttccccgct aacccgtgat atcagagtta actgaatttt
    36121 tcttaccgcg tctgccgtta cacttggaac gttagggagg gcgtattgcc ctccagacca
    36181 ggaaagtctt cgggatatgt ctatgaaaaa actgttactg atctgtttgc cggtccttct
    36241 caccggctgt agcgcgttca atcaactggt tgagcgcatg caaaccgata cgctggaata
    36301 ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat ccgcgccagg aagtcagttt
    36361 tgtttacgat aatcaactac tgcatctcaa acagggcatt tctgcgtctg gtgcacgtta
    36421 caccgacggg atctatgttt tctggtcgaa aggcgaggaa gcgactgtct ataaacgcga
    36481 ccgcatcgtc ttgaataact gtcagttaca aaatccacag cgttgagatt tttccagggg
    36541 cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa aaccatgtct gataacgacg
    36601 aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa aggcggatta cgccgccgcg
    36661 atcttcccgc cgatccatta accctttttg aacgctggct ctctcaggcg tgtgaagcca
    36721 aactggcgga ccctaccgcg atggtggtcg ctaccgtgga tgaacatggt cagccttatc
    36781 agcgcatcgt tttactcaaa cattacgacg aaaaaggcat ggtgttttac accaacctcg
    36841 gcagccgtaa agcacatcaa atcgaaaata atccgcgcgt tagcctgctg ttcccgtggc
    36901 atacccttga gcgccaggtg atggtgatcg gtaaagcgga acgactttcg actctcgaag
    36961 tgatgaaata ttttcatagt cgtccccgtg atagccagat tggtgcatgg gtttcgaagc
    37021 agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga
    37081 agtttcaaca gggcgaagtg ccattgccga gtttttgggg cggttttcgc gtcagccttg
    37141 aacagattga gttctggcag ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc
    37201 gtgaaaatga tgcgtggaag attgatcgtc ttgccccctg aaaagatgca aaaatcttgc
    37261 tttaatcgct ggtactcctg attctggcac tttattctat gtctctttcg catctggcga
    37321 aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg gagattttga tggcaagcag
    37381 taacttgatt aaacaattgc aagagcgggg gctggtagcc caggtgacgg acgaggaagc
    37441 gttagcagag cgactggcgc aaggcccgat cgcgctctat tgcggcttcg atcctaccgc
    37501 tgacagcttg catttggggc atcttgttcc attgttatgc ctgaaacgct tccagcaggc
    37561 gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt ctgattggcg acccgagctt
    37621 caaagctgct gagcgtaagc tgaacaccga agaaactgtt caggaatggg tggacaaaat
    37681 ccgcaagcag gttgccccgt tcctcgattt cgactgtgga gaaaactctg ctatcgcggc
    37741 gaacaactat gactggttcg gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa
    37801 acacttctcc gttaaccaga tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga
    37861 agatcagggg atttcgttca ctgagttttc ctacaacctg ttgcagggtt atgacttcgc
    37921 ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt ggttctgacc agtggggtaa
    37981 catcacttct ggtatcgacc tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac
    38041 cgttccgctg atcaccaaag cagacggcac caaatttggt aaaactgaag gcggcgcagt
    38101 ctggttggat ccgaagaaaa ccagcccgta caaattctac cagttctgga tcaacactgc
    38161 ggatgccgac gtttaccgct tcctgaagtt cttcaccttt atgagcattg aagagatcaa
    38221 cgccctggaa gaagaagata aaaacagcgg taaagcaccg cgcgcccagt atgtactggc
    38281 ggagcaggtg actcgtctgg ttcacggtga agaaggttta caggcggcaa aacgtattac
    38341 cgaatgcctg ttcagcggtt ccttgagtgc gctgagtgaa gcggacttcg aacagctggc
    38401 gcaggacggc gtaccgatgg ttgagatgga aaagggcgca gacctgatgc aggcgctggt
    38461 cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa accatcgcct ccaatgccat
    38521 caccattaac ggtgaaaaac agtccgatcc tgaatacttc tttaaagaag aagatcgtct
    38581 gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa
    38641 ataatgcatt aagtggaaag ggggagtgag aaatcactcc ccctggtttt tatacaggga
    38701 acatgatgaa aaatattctc gctatccagt ctcacgttgt ttatggtcat gcgggtaaca
    38761 gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc
    38821 aattttctaa tcacactcaa tacggcaaat ggactggctg cgtgatgccg cccagccatt
    38881 taaccgaaat tgtgcaaggc attgccgcca ttgataaatt acacacctgt gatgccgtat
    38941 taagtggcta tctgggttcg gcggagcagg gggaacatat cctcggtatc gttcgtcagg
    39001 tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa
    39061 aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt gcgtcacggt ttgcctgcca
    39121 gcgatatcat tgcaccgaat ctggttgagc tggagatact ctgtgagcat ccggtaaata
    39181 acgtcgaaga agcggttctg gcagcgcgtg aactcattgc gcaagggcca caaattgtgt
    39241 tggttaaaca cctggcgcgg gctggctaca gccgtgaccg ttttgaaatg ctgctggtta
    39301 ccgccgatga agcctggcat atcagccgtc cgctggtgga ttttggtatg cgccagccgg
    39361 taggtgttgg tgatgtgacg agcggtttac tgctggtgaa actgcttcag ggggcaacgc
    39421 tgcaggaggc cctggaacat gtgaccgctg cagtatacga aatcatggtg accaccaaag
    39481 caatgcagga atatgagctg caagtggttg ctgctcagga tcgtattgcc aaaccagaac
    39541 attatttcag cgcaacaaag ctctgaaata tctcatttcg gcccacaacg acctgtgggc
    39601 cgatggctat tactttaagc cttccgctga cagcgcgtct tgtacttccg gacgttcagc
    39661 catacgttgc ataaatgccg caatgtgctc taacccttcc agattcagtt tcaccgcgta
    39721 tgcccagcgc agaaccgtaa acagataggc atcagcaatt gtaaatcgtt gcccgcagat
    39781 ccagtgctca tccttcagag catcgttcac atattgcagc ttcttctcca gctgcgcgcg
    39841 aactgttggt ttgtactctt ccggtgtatc agggcgaaac agaggtgtga agcctttatg
    39901 cagctcggtg gcgatgtaat tcagccattc gatggtttta tagcgggaaa tactgtttac
    39961 cggtgccagc aactggcggt cggggacgct gtcggcaaga tactgcataa ttgctacgcc
    40021 ttccgtcagc aaagtaccat cgtccagcag caatgcaggc acctgtccct tagggttaac
    40081 ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt aaatccacac tgacgagcgt
    40141 aaaatccttt ccgctctcac gcagggtgat atgggaagcg agagagcagg cgcccggttt
    40201 gtagaacaat ttcatcggta actccttttg tctgtggttt aaagtatggt agtgcgttgc
    40261 tgaacaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt ccctgaattt
    40321 tacgctacag cagctttcgc cgctttgtct gcagcgtcat cctgcgtcat gcggtgcagt
    40381 ttcggcgcgg tcagcagcat cagtactgca ataacggcag tagcgacacc aatctgcaag
    40441 aatacgcgac catagacttc cagtgacatc agtggatcgg taacgttatc cggcacagcc
    40501 atcatacccg caacataacc accaatcagg tttgcaccgg cagtggtcag gaaccagcta
    40561 cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc cagacccaga
    40621 ccagagatca taagttcccc gatactctgc aggccatagc ttgcgaccag ccagcttaca
    40681 gacacgatac ctgcgtcaga cgcgaatttt gcacccagcg gcagaatcag gaacgcacca
    40741 gaacacatca ccataccgat tgcaaactta gttggcatcg gcagggtatc gcccatcttg
    40801 ttatagatag cggccagaat cgggctacca atgatgatcc agaacgggtt cagtgcctga
    40861 tactgctcag gttctacggc cagacccaga atggagtgct caacgttacg aatcgcaaag
    40921 aagttcagtg acgttggcat ctggctgtac agcacgaaga agataatggc ttcgagcatc
    40981 aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta
    41041 ccgaagataa ccacgatacc aaaggcaaca acgcccagcg ccatacgcgc aacttcctga
    41101 ttgtgcagca gccaggtggc gatagcgatc agtgccacaa caccaataat ggttagcagc
    41161 aggttacggt agttgatagg ctcgaagtct ggttttgaac cgtactgctt aacccagcgt
    41221 tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa cgcaaacgca
    41281 acactccagc cgtacttcgc ggccagccac ggggtggcga tcatagagaa gaaagagccg
    41341 atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgcgggtc gtttttctca
    41401 tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc gaccgcaata
    41461 gccgccatac ccatataaac gataccggcg tcgtgaccag accaggcaac cagagcataa
    41521 ccaatcgcca gcacaatagc gccaagcata attacgcgtt tagtacccag taccttgtca
    41581 cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga agagaaaagg
    41641 gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc cataattcct
    41701 tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag atagaacgcc
    41761 ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg gtttttggtt tgcagtggac
    41821 acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg ttatgcctaa
    41881 aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt cactatcttg
    41941 cactttcaga caagtacttt gtaataatct gttacatata actgaggcgg ggaattcaac
    42001 cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc agattttttc
    42061 actaccctga gagggggatg atggcttaac ggcgatatgt tctgctattt agcgtttttc
    42121 ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt
    42181 gttgcgtgtg taaatttaaa tgattttaac ctagttcttg caatgttttt acattgccac
    42241 agcatcgtga tcttgatcac gaatgaatag aaaatttcgc tattaaaaaa acaattaacc
    42301 tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa tggcatcatt
    42361 gacgttaaaa agatgtttta aaagaggata aagaaaggtt atgaatgggg taatcggtgt
    42421 tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt caataataca
    42481 agagccacag cgaggcttgc gggcaataca ggtataacgc ccgtgcagga tcaaccaatg
    42541 gtggcagtcg attttaaact ctgccggaac cactttcagt agcttttctt ctacctgttc
    42601 gacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt gcgtgtcgac
    42661 agcaatagtc ggccagccga atgcagtgtt taatacgacg ttggctgttt tacgacctac
    42721 accaggcagg gcttcaagcg cagcacgatc ttccggaacc tcgccattat gctgttccag
    42781 taagatacgg caggttttga tgatattttc tgctttgctg ttataaagcc caatggtttt
    42841 gatataggtt ttcacccctt cgacgcccag ttcaagcatc gctgcaggtg tattagccac
    42901 cgggtagagt ttcgccgtcg ccttattaac actgacatcg gtcgcctgag cggaaagcag
    42961 tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcggtggtgg gatgaggatt
    43021 gttctcacgc aggcgagtga ggatctccag gcgttttgct ttgttcatca gacattccct
    43081 gtttcaccgt ttggcaatgc acgttcggca gctgcttcag tacggcgctt tttcattctt
    43141 tcatcaatca ggtattttcc tgccagcatc agtcccaggc caataaatgc acctggtggc
    43201 agcatcgcca gcaggaaagg ggagtcggtg tggaaaattt ccacgcgtaa tacttttgcc
    43261 cagctaccta acagcgcatc tgcaccgtca aacaacgtgc cattgccgat tatttcgcgt
    43321 agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt
    43381 gccgaaagcg ccggaccttt tttggcggcg aaggcttcag cgcggcccac aacgatacag
    43441 ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa ggcgtaggcg
    43501 ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta aatgggaatg
    43561 cggatctcgg ctggcgtcaa gtgacgcagc gtcgaaatgg tcaagttggt cagcgtcagt
    43621 accagcgtag tcgcaagtcc taaacccaga gcgttagtgg cagtggacgt gaccgccaat
    43681 agcggacaga ggccgagcag ctggaccagc gcagagttgt ttttccacaa cccctgaaca
    43741 ataacgtctt taatttcgct cacgctttat tctccacagg caggaagttg agaaagttgt
    43801 gacggtaacg tctgagcgta caatccggcg cgttttaccg cattaaccac cgcgcgggga
    43861 gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc
    43921 gcatcatccg caccactgat ttttttaccc gcaaaatggg tgatccagtc agaaaggcgt
    43981 agttcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg cgttccaagt
    44041 accgtgccgt taaaatcggc tcccaccagc agctgaatcg caccggaata gccatctggc
    44101 gcggttgttt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat gtaaacccga
    44161 tgctcacctt tacctaactc tggcgcagtg accagatagc aactctgtgc cagcgcattg
    44221 ttatagcgtt cggctggcag cacctgatca aataacgcct tttgttgcag actggcctgt
    44281 tcagcaatcg tcgttttggt catctggttg atggccgcag ttaaccctgt tgaacccgct
    44341 gcaaatagtg ccagtgtaat gccgtgtttt cggatagttt tcagcatggt ttaccctttg
    44401 cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc
    44461 agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat caaccagact
    44521 aataagcccg ccagcgcgcc gaaaataaga cgaccacgat tggtcgtaga agcggtaacc
    44581 gggtcagtca aaataaagaa tgcgccgagc atagtcgcac cagacagcag atgaatttgc
    44641 ggtgatgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt
    44701 actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa ccacacgccg
    44761 ccagccagcc aggcgagatt tacccattgc caaccagcgc ccgccagaat accgctgtag
    44821 atcggatatt gcataatctg ttcaaccgaa tgaccggcac gcacagaggt tttaaatgta
    44881 tccagcggtg tcgcctgact aatgccatca atacctaagc gtagtgtgtt catatcacca
    44941 ccactggcgg tatgtccgct gaaaataacc tgtatggcgt cgataaaacc agggatgttg
    45001 accgcaatgt catgcggcgg taaccagctg gtcatctgca ccgggaagga gatcagtaag
    45061 accacataac caatcattgc cggattaaac gggttttgcc ccagaccgcc atacaactgt
    45121 ttagcgatga tcaccgcaaa caccgtaccc agtacgacca tccaccatgg cgcgaggggg
    45181 ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt caacgttgcg
    45241 gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagc actgaccgac
    45301 gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct
    45361 ggcacagctg cgagcaacac caacagcata atgcgcgatg tttggcgctg gttatgggta
    45421 taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt ttgctgggcg
    45481 gcttttttgg cctgaacgcg ggcaatagcc gcagcaactg ccgctttgcg cggatcaatc
    45541 tgttcttctg gttctgcgtt agcctgttgc tgttccagct tgcgtgcttt ggcacgggca
    45601 atagcggctt cgacggcggc tttgcgcgga tcgacctgtt cttctggttc cgcattggcc
    45661 ttttgctgtt ccagcttgcg tgctttggcg cgggcaatag cggcttcgac ggcggctttg
    45721 cgcgggtcaa ccttttcttc tggctccgca ttagcctgtt gctgctccag cttgcgcgct
    45781 ttggcgcggg cgatagcggc ttcgacggcg gctttgcgcg ggtcaacctg ttcttctggc
    45841 ttcgcattag cctgttgctg ctccagcttg cgtgctttgg cgcgggcgat agcggcttca
    45901 actgcggttt tacgtggatc agcaacggtt gctgcgtcgt tagtttgctg cagttctgcc
    45961 tgtttcgctc tggcttgcgc tttacgggct tctcgtactg caataattgc actgttatcc
    46021 gggcgttcgc ccgctttaat cacaatcggc tgtgtagcct gggcctgttt ctctttcacc
    46081 cgcgccagag cggcagcaat cgcatcttta tctttggctg caggttgaac ggctgcgctc
    46141 ttatgtcgtt caaggcgagc cgctttttcg cgctccagac gagcctggcg cgcttcgaaa
    46201 cgcgctttgg cttctgcggc gcgcttttct tcctgatgaa tagccgcaat ttcagctttt
    46261 tcctgacgga aatattgcac caggggaata ttgctcgggc aaacccacgc gcaagcccca
    46321 cattcaatgc agtcggcaat gttatgcgtg gtagctttat cgtgttgctg acctttgctg
    46381 aaccagtaca actgttgcgg caaaagatcc gcagggcagg cgtcagcaca ggcgctacac
    46441 cggatgcagc tttgttcttc ctgtggttcg ccaagctcat tggcagaggg agccaacaga
    46501 cagttggtaa tctttacgac cgggacatcc agccatggca aggtaaagcc cattagcggg
    46561 ccgcccataa tcaccatttg atcggcagag gggcagaatc cggcgtcatt caataaatga
    46621 cgcactggcg tccccagccg tgcccagacg ttgcccgggc gagcgattgc ttcgccagtc
    46681 agggttacga cacgctcggt aatcggctcg ccatcaataa cggcacgttt cactgcataa
    46741 gcagtgccga cgttttgcat taatacgccg atatcggatg aacgcccgcc atgtggaacc
    46801 tgcttcccgg tcagaatgta ggttaattgt ttagcaccgc cagaaggata tttagttgga
    46861 atcacccgca gagaaatatc gtgagagtcc gccagcaccg cgcggagcat ggaaatcgcc
    46921 tgcggtttgt tatcttcaat gccgataaga atttcgcgtg gctgcagaat atgcgcaaga
    46981 atgcgaatac cctctacgac ctgagccgcg caatcctgca tcaaacggtc atcggcggta
    47041 atgtacggct cgcactcagc agcgttgata atcaacgttt caatcttatc tccgccgccc
    47101 tgcaatttaa cgcctgtcgg gaatcccgca ccacccagcc cggcaacacc aaactgatga
    47161 atgcgctcga ttaactcttc gcgactgcga gagcgataat cgtcccagcc gtcgcgcggg
    47221 atccagcagt cttcgccatc agcatcaata atcacgctta attcagctaa agctgaagga
    47281 tgagccgtag agtggggcgc aatagccgta acggtacccg aggtgggcgc gtgaactggc
    47341 agcattttgc cgcgaccacg ggtaagcggc tggccgcgca gtactttatc gccgacgcta
    47401 acgcacaact caccttcaga gccaatatgt tgtttcagtg gtatgacaaa acgctgcgcc
    47461 aggggtacct ggcgcagggg cgtaccgttg gactgggttt tcatctccgg tggatggatg
    47521 ccgccgttga aatcccagat tttatttttt ctgaatgcag agaataactt aagcatggtg
    47581 ttccacggga atgatacgca cgggaatggt gttcagatcc catttccagg agtcaggtgt
    47641 ttctgcaacc ggttgcaacg agatgcagtg cgtcgggcac ggatcaacac ataaattgca
    47701 gcccgtacag agatcactca ttaccgtatg catggcgcgg gtagcgccaa cgatggcgtc
    47761 taccggacac gcctgaatac atttagtgca gccaatacag ttattttcat caataaccgc
    47821 caccatccgc gtaggcgtta gctcttgcgc ttcgccatcc agcggctgtg gctcgacatt
    47881 aagcaactca gcaattttta gcatcacagc ttcgccacct ggggcgcagc ggttaatttt
    47941 ttcaccgtta cagctgatgg cttccgcgta ggggcgacag ccgggataac cgcactgacc
    48001 acactggctc tgcggtaaga tttcgtcaat tttctcaacg accggatcgt cttccaccgc
    48061 aaaacggcgg gaggcataac ccagaatggc gccaaacgcc aggcccagca ggctcacggc
    48121 ggcaacggca atccagatag cattcattac aacttcacca aaccactaaa gcccataaag
    48181 gccagagaca taagacctgc ggtaattaac gcaatggcat taccgcgaaa aggtgccggg
    48241 acatcagcca cagcaaggcg ttcgcggatg gcggcgaaga gcaccatcac cagcgagaaa
    48301 ccgacagcgg cggaaaaacc gtacagcgcc gactgcaaga aattgtgccc gagattgata
    48361 ttcagcaacg ccacgccgag cactgcacag ttggtggtga taagcggcaa aaaaatcccc
    48421 agcagacggt aaagcaccgg gctggttttg cgcaccacca tctcggtgaa ctgcacaacc
    48481 acagcaatca ccagaataaa cgccagggtg cgcaggtaaa tcagattaag cgggatcaaa
    48541 atccacgtat cgataagcca ggcgcaaata gacgccagcg tcatcacaaa cgttgttgcc
    48601 agccccatgc ccatcgcggt ttccagcttt ttggaaactc ccataaacgg acagagaccg
    48661 agaaacttga ccagtacaaa gttattgacc agtacagttc cgacaaacag taacaggtag
    48721 tcagtcattt ttcggcctga aataaaaaag ccgcctatta tccgttaatc cagagcaggc
    48781 gacaacaggt taactgtaag gttattacgg attcacaaac gtcgctttca cccgcgacga
    48841 gcgtttaaaa taggggacga tcaatgcggt tgccaacaaa gtaaacagta actggcgaac
    48901 ggctatgcca tcttccacgg gtgaaaaagc gaacgctttt actgccagca atacggaaat
    48961 aagcagccag ataatatagt gtttaggaac acaacgccga cgtttaaaga aagcgatggt
    49021 caaccacagt gtgtaatacc acatggcgat agcggtaata aaagaaacgc cccataagat
    49081 ttgtgtcgtc agtgcttgtt cgccgagcgt ttgaaatgtt tgcggagaag ataacgcagc
    49141 ggtatacagc aacaacgcca gcgtcgtact taacagagcg actaaaagcc aggccaatgg
    49201 gccaagcaac cagcctccaa tacgttgtgg cgttgtggtg gtcataggtt ctccagaata
    49261 gcgacgcaaa caggcaaatc taattttagg ggcgttatta taaggctttt ataaccgatc
    49321 gctactttct ttattggaca tagtgccaga cggacttcgg tacctggccg aggtcaaaca
    49381 aacgcccgcc agaaaccagc tctgcgcgac ggtgatcggc agcacgatac atattgatca
    49441 gttcctgatc gtccgtcagg gtgtaattaa gatggtcgta gagtttttcc agactttcga
    49501 gtgaactgat tttgcgaaat tttaataagt agtcctgaac agtcataaaa ggtccataaa
    49561 ataattaata ccaaaggggt aggtattatt agtgatgata aaatttcacc aaaagtgaac
    49621 gcttgtgtta ttacaataat aagaatgcgc tgatggcaaa tattcaggga tgaaaaactg
    49681 ctctgaaaag agtaacgcgc tttactatct ctgacaatca ccaacaacat cgaacaagat
    49741 aataaattcc tggtttaata tccgacaagt gaaaacatgc acccggacgg gcagcatgtc
    49801 gctctacaag cacagagctt acttgtgttg taccgtagta ctctgttcag gtggctgata
    49861 gttgtcaatg tgactcgcca cgccaagaag aatgactgaa acgacaagaa cgatccaacc
    49921 tgttaattca ataagacgat tcattacagc ccacatctct gttgattgat ccattaactt
    49981 cagggggtaa atgttactta gcaataatag ctccagcaag atttttactg aggtttttgc
    50041 gatatttagc ttttgtcgtt ggaaaattcg ctattttttt gacttaagtt aaacaacatc
    50101 ccttattgct ggcaggttat taaactgttg agcgagggta aaggatagtg tcaaatagcc
    50161 atcatacttc aatgagaggc aatgacatga gcgacaacat ccgtgttggg ttgattgggt
    50221 atggttatgc gagcaaaacc ttccatgcgc ccctgattgc gggcacgccc gggctggaac
    50281 tggcggtaat ctccagcagc gatgaaacaa aagtaaaagc cgactggcca acggttacgg
    50341 ttgtctctga gccgaagcat ctgtttaacg atcccaacat agacctgatt gtcatcccta
    50401 cacccaacga tacccatttc ccgttagcca aagcggcgct tgaggcgggt aaacatgtgg
    50461 tcgttgataa accctttacc gtgacactgt cacaagcgcg agagctggaa gcgctggcaa
    50521 aaagcctggg gcgtgtgctg tctgtattcc ataaccgtcg ctgggatagc gatttcctga
    50581 cgctaaaagg tttgctcgcg gaaggcgtac tgggtgaagt tgcttacttt gagtctcatt
    50641 ttgaccgctt ccgtccgcag gtgcgcgatc gttggcgtga acagggcggc ccaggcagcg
    50701 gtatctggta cgatttagca ccgcaccttc ttgatcaggc cattacgcta tttggtttac
    50761 cggtcagcat gacggtagat ttggcacagt tacggcccgg agcgcagtcg accgattatt
    50821 tccacgccat cttgtcctat ccgcagcggc gagtcatttt acacggtacc atgctggcag
    50881 ccgctgagtc agcacgttat atcgtgcatg gatcccgagg cagttatgta aaatatggcc
    50941 tcgatccaca ggaagaacgt ctgaaaaatg gccagcgtct gccgcaggaa gactggggct
    51001 acgatatgcg tgatggcgta cttacccgcg tggaaggtga ggaacgtgtc gaagaaacgc
    51061 tgttgacagt accagggaat tatccggctt actatgcggc tattcgtgat gcgttaaatg
    51121 gcgatggtga aaatccggtt ccggcaagtc aggcaatcca ggtaatggag ttgattgagc
    51181 tgggcatcga atccgccaaa catcgcgcga cgctgtgcct tgcgtgaaaa gaggatggtg
    51241 caattagcac catccatttt tgttacttcg cagcgacttt ttctcgcagt gcgcgttttt
    51301 cctctgcgct gaggaaagcc atttccagac cattaatctg cgcctggcgg atttgctcgc
    51361 gggataaccc agcagccggc gcggcaacgg tatattcgtg aatgatatcc actccctgta
    51421 cgccgggatc gtcagtgtta atgctggcac gaatgccatg ctcaaggaac gttttcagcg
    51481 gatgtgccgc cagctctgcc acggtgctgg tctgaatatt ggaggtcaga caggattcaa
    51541 taccaatttg ctgctcggcg agaaaatcca tcagcgcccg atcttcaatg gcttttacgc
    51601 catgtccaat acgttccgcc cccagttcac gaatcgcctg ccagatgctt tccggcccgg
    51661 cagcttcgcc tgcatggacg gtaatatgcc agcccgcatc acgcgcgcgg ttgaagtgag
    51721 aaaggaacag acttcccggg aaaccaagtt catcaccggc taaatcaagt gcggtaatct
    51781 ggtctcggtg ggctaaaaag gcctccagct cttgctgaca ggcggcttca ccgaaggtcc
    51841 ggctcataat gccgataagc ttcgcctgta caccaaaggt gcggcaaccc tcacgtacgc
    51901 catcgatcac cgcttcgaca acacccgcta caggcagctg atgtgccatt gccatgtagc
    51961 ccggtgaaaa acgcagctcg acatagtgca ggccgttacg ggctgcatct tcaatgtttt
    52021 caaatgacac gcggcgacag gcatcaagag aggcgagaac tttaacgccc cagtcaagtt
    52081 tggtcagaaa gctcacaaga tcgggttcgt tggcaatgac ctgaacgtgg ggaatcagtg
    52141 tttctaggga ttgtgcagga agcgagatat tatactggcg gccaagttca agaatggtct
    52201 ggggacgaat gttgccatca aggtggcgat ggatatcagt taatggcagg gtggtatcaa
    52261 tcatggtcgc actctttttc tggatataaa agtgcgcacc attataaaaa caaagcaggg
    52321 taaaaatcca cgattgcgca acgatttttt acgttgcgca attggggtta acgaacagcg
    52381 cggatggcgt taattaatcc agcaacacct ttttccagtt tcgaacgcgg gcacccggca
    52441 ttgagacgga caaaaccacg accttcttca ccgtaggtat accccggcat gatcgcgacc
    52501 ttctcttgct cgataagcgc tttttgcaac gcgttgtcgt caatattcaa cggacgtaga
    52561 tcaatccagg ccagataagt ggattgcggg atctgccagt tgagttcagg gaacgcggcg
    52621 ttcattttat ctgcgatata cgtcaggtta tctttcagat agacgcgtaa ggcatccagc
    52681 cacggcgcgc cttgctgata ggcggcgata tgggcagtta acgccagtac tgaaggggaa
    52741 gaaaggccat cacgtccttt cagtgccgat aaataggcat cacggctgct gctattttct
    52801 ataatcccgt aagcaccggt cagggcggga atattgaaac ttttcgatcc cgacgttagc
    52861 aatgcccagt ctccgcgcgc cacattactc cagggaatat gcggctgctc ggcccaaacc
    52921 atatccatat ggatttcatc ggaaataacc cgcacaccat gacgctcgca caggtcagcc
    52981 atgatctcca gttcatcgca cgtccacact ttcccggtag gattctgtgg gctacacagg
    53041 agcataattt tacattctgg tttcgccaac acggcttcaa gcttgcccat atcgcaaaac
    53101 cagccgtcag cccgcttctc taaagcgacg ggcattactg tgcgctggtt accttcaatg
    53161 gccttgtaaa atgcgtcata ggcgggtgtg tggatcacca cgccttcacc tgtttcagac
    53221 cactgacgaa tcagttctga aaccatatag ataacagaag ggccatacac caccgactgc
    53281 gtatcaattg cggtgtaatg ctgggtggaa aaccagtggg caatagccgc gagaaactca
    53341 tcgtttttcc agcggctgta gccaaacacg ccgtgcatca ggcgctgatt cagcgcctcg
    53401 ataatgcagg gggcagtggc aaaatccatg tctgaaatcg tgaacggtaa caggtcagca
    53461 gtgccgaaac ggtcagcgac ataatcccac tgcgtacacc atgtgccatg acgatccacg
    53521 acctttgaaa aatcgaacat atcttatcct tatgcctgga cagtatgcat cagaccggcc
    53581 atttcatctt ttactgactg aacttgtggc ccgataacaa cctgcaggtt atgttgatta
    53641 agttgtacta cgccaattgc ccgattgtcc ttcagtgcct gcacattaac aagcgacata
    53701 tctttcacag acaaacgcag acgggtaatg cagttatcga ggctgacaat attgtcggca
    53761 ccgcctaagg cttcgaggat tgccggaacg ttgtaacctg atttacccgg cacaccggca
    53821 acggcttttt cgattgagct ggcaacttcg ctatcgcgcc ccggggtttt cagattgaag
    53881 cgggtgatag cgaaacggaa gatgacgtag taaacgacaa accagattgc cgccaccact
    53941 ggcaccatgt accacttggt tgacagacca tgcaaaatac cgaacaccac gaagtcgatg
    54001 atattgccgt cggtattacc gatggtgacg ccgagcacag acatgacggt gaagccgagg
    54061 ccggttaaca gcgcgtggat gacatacaga actggcgcta cgaacaggaa caggaattcc
    54121 agcggttcgg tcgtgccgcc aacgacgcag gcgatcaggc cagaaatcag cagaccttta
    54181 attttatggc gattttccgg gcgcgcgcag tgatacatag ctaacgctgc acctggcaga
    54241 ccgccgagaa acgcaggcat tttaccttgc gaaaggaaac gcgtggcgct ttcagaaaaa
    54301 ccgtgagtgg tcgggcaact caattgcgcc tggaagatgg tcagtgcgcc gctgacggtt
    54361 tgaccgcaga cttcctgcgt gccgcctgcg tcggtaaagc gaattaatgc caccagaatg
    54421 tgatgcagac caaacggcaa cagcagacgt tcaccggtac caaacagcat cggtccgaaa
    54481 tcacccgcac tgttgatcat atggcccagg ccgctaatac ccatggcgaa aatcggccag
    54541 actaatggaa tcaccagacc gacaaggccc atcaccagcg aggagatgat tggtacgaag
    54601 cgcgtaccgc cgaagaatgc cagcgcatcc ggcaggcgga tattatggaa tcgctcatgc
    54661 agcatccaga cgataatacc ggcgatcacc gcaccgagga tcccggtatc gatcgactgg
    54721 atcccaagaa tgctctgtat gttattggtt ttcagaacgg cggcgtctgt ggttggcaga
    54781 atgcctttat tggttaacca gaagtttacc gcgaggttca ttaccgcata accgacgaag
    54841 ccagcgaatg ccgctacgcc tttgttttcg cgtgccaggc ccagcgggat ggcgatacag
    54901 aacatgactg gcaggaaact aaaagcaaac gagccaatct tactcatcca ggtaaagata
    54961 gcctgcaaca cggggttgcc caggaccggg attagggtga tgacatcgtg gctactaagg
    55021 gaactaccaa tgccgagcat aatgccgcag aacgacaata atgccacggg taacatgaag
    55081 gttttgccta actgctggaa gaactcccac agcgtgactt tcggtgctgt tttcgccgtc
    55141 ataaaacgac tcctcgtaga gaacaatctg gattcagtaa attgcgcgaa aagataaaac
    55201 gttttatcaa attttagtga ggcataaatc acattacgca acgataatag cgggtataag
    55261 ataaataaaa ggtaaaacgt tttatctgtc acataatcag ggagtaggtc atctgcatgg
    55321 ctaccgccaa aaaaataacc attcatgatg ttgcgctggc tgcgggcgtg tcggtaagta
    55381 ccgtttcgct ggtgcttagt ggcaaagggc gaatctctac cgccacagga gaacgcgtta
    55441 acgccgccat tgaagagctg ggatttgtgc gcaatcgcca ggcgtcggcg ctgcgcggcg
    55501 ggcaaagcgg cgtcattggt ttgatcgtcc gtgatttatc tgcgccgttt tacgccgaat
    55561 tgacggccgg attgacggaa gctctggaag cgcagggacg gatggtgttt ttgcttcacg
    55621 gcggtaaaga cggcgagcag ctggcacaac ggttttcact gttactgaat cagggggtcg
    55681 atggtgtggt aattgccggg gctgcaggaa gcagcgatga cctgcgacgg atggcagaag
    55741 aaaaagctat cccggtgatt ttcgcttccc gtgccagtta tcttgatgat gttgatacgg
    55801 ttcgcccgga caacatgcag gctgcacagt tgttgacgga gcatctcatt cgcaatgggc
    55861 atcagcggat cgcctggctg ggagggcaaa gttcctcatt aacccgtgca gaacgggtgg
    55921 ggggctattg tgcgactcta ctaaaatttg gcctgccgtt tcacagtgat tgggtgctgg
    55981 agtgcacttc cagccagaag caagccgcgg aagctatcac ggcgctttta cgtcataacc
    56041 cgaccatcag tgccgtggtt tgctataacg aaactattgc gatgggggca tggtttggtt
    56101 tgctgaaagc agggcggcaa agcggggaaa gcggagtcga tcgttacttt gagcaacagg
    56161 tttcgctggc ggcatttacc gatgcgacac caaccacact tgatgatata cccgttacct
    56221 gggccagcac gccagcgcgg gaacttggta ccacacttgc ggatcgcatg atgcaaaaaa
    56281 tcactcatga agaggcgcat tcacgcaatc ttattattcc cgcccggctc attgcgccga
    56341 aataatcctc tctttatctg ctatacctgg tagtgtcctt tcctcaaggt taatgataaa
    56401 aaatgtggca cacaggcggt gccttttatt tttactgaag ttcacaggag gtttatgtgt
    56461 ttaattctga caacctgaga ctcgacggaa aatgtgccat catcacaggt gccggtgcag
    56521 gtattggtaa agaaattgcc attacattcg cgacagctgg cgcatctgtg gtggtcagtg
    56581 atattaacgc cgatgcagct aatcatgttg tagatgaaat tcaacaactg ggtggtcagg
    56641 catttgcctg gcgctgcgat attacttccg aacaggaact ctctgcactg gcagactttg
    56701 ccgtcagtaa gctgggtaaa gttgatatcc tggttaacaa cgccggtggc ggtgggccta
    56761 aaccgtttga tatgccaatg gcagattttc gccgcgctta tgaactgaat gtattttctt
    56821 ttttccatct gtcacaactt gttgcgccag aaatggaaaa aaatggcggt ggcgttattc
    56881 tgaccattac ttctatggcg gcagaaaata aaaatataaa catgacctcc tatgcatcat
    56941 ctaaagctgc ggccagtcat ctggtcagaa acatggcgtt tgacctgggt gaaaaaaata
    57001 ttcgggtgaa tggcattgcg ccgggggcaa tattaaccga tgccctgaaa tccgttatta
    57061 caccagaaat tgaacaaaaa atgttacagc acacgccgat cagacgtctg ggccaaccgc
    57121 aagatattgc taacgcggcg ctgttccttt gctcgcctgc agccagctgg gtaagcggac
    57181 aaattctcac cgtctccggt ggtggggtac aggagctcaa ttaatacact aacggaccgg
    57241 taaacaaccg tgcgtgttgt ttaccgggat aaactcatca acgtctcagc taaataactg
    57301 gcagccaaat cacggctatt ggttaaccaa tttccgagtg aaaagtatac gaatagagtg
    57361 tgccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
    57421 attcacgcta actataatgg ttttatgatg gataacatgc agactgaagc acaaccgaca
    57481 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
    57541 tcgatgaaag ccatctgtaa atcttgcgcc attagtcccg ggacgctcta tcaccatttc
    57601 atctccaaag aagccctgat tcaggcgatt atcttacagg accaggagag ggcgctggcc
    57661 cgtttccggg aaccgattga agggattcat ttcgttgact atatggtcga gtctattgtc
    57721 tctctcaccc atgaagcctt tggacaacgg gcgctggtgg tcgaaattat ggcggaaggg
    57781 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cacggaattt
    57841 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
    57901 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
    57961 gaagatctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtatc
    58021 ttaaccgcat cctgatcctc tctctctttc cgcgggctgg tgacaactga gcccgcgttt
    58081 catatcgtaa tttctctgtg caaaaattat ccttcctggg ttcggagaat tccccccaaa
    58141 tattcactgt agccatatgt catgagagtt tatcgttccc aatatgctcg aacgaacgtt
    58201 cgattgctta ttttgtggct tatgtcaacg ctgttttaaa gattaatgcg atctatatca
    58261 cgctgtgggt agtgcagttt ttggtttttt gatcgcggtg tcagttcttt ttatttccat
    58321 ttctcttcca tgggtttctc acagataact gtgtgcaaca cagaattggt taactaatca
    58381 gattaaaggt tgaccaatat ttttatctta atgaggagtc ccttatgtta cgtcctgtag
    58441 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
    58501 aaaactgtgg aattgatcag ctttggtggg aaagcgcgtt acaagaaagc cgggcaattg
    58561 ctgtgccggg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgtgggca
    58621 acgtctggta tcagcgtgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
    58681 tgcgtttcga tgcggtcact cattacggca aagtgtgggt aaataatcag gaagtgatgg
    58741 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
    58801 aaagtgtacg tatcaccgtt tgtgtgaaca atgaactgaa ctggcagact atcccgccgg
    58861 gaatggtgat taccgatgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
    58921 actatgccgg gatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
    58981 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
    59041 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
    59101 ctggacaagg caccagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
    59161 aaggttatct ctatgaacta tgcgtcacag ccaaaagcca gacagagtgt gatttctacc
    59221 cgctgcgcgt cggcatccgg tcagtagcag tgaagggcga acagttcctg atcaaccaca
    59281 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggatttgcgc ggcaaaggat
    59341 tcgataacgt gttgatggtg cacgatcacg cattaatgga ctggattggg gccaactcct
    59401 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
    59461 tcgtggtgat tgatgaaact gcagctgtag gctttaacct ctctttaggc attggtttcg
    59521 aagcgggcaa caagccgaaa gaactgtata gcgaagaggc agtcaacgga gaaacccagc
    59581 aggcgcactt acaggcgatt aaagagctga ttgcgcgtga caaaaaccac ccaagcgtgg
    59641 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
    59701 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgtgtcaatg
    59761 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
    59821 accgttatta cggttggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
    59881 aaaaagaact gctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
    59941 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
    60001 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
    60061 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
    60121 acaagaaggg gatcttcacc cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
    60181 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatgaatca
    60241 acaactctcc tggcgcacca tcgtcggcta cagcctcggt gacgtcgcca ataacttcgc
    60301 cttcgcaatg ggggcgctct tcctgttgag ttactacacc gacgtcgctg gcgtcggtgc
    60361 cgctgcggcg ggcaccatgc tgttactggt gcgggtattc gatgccttcg ccgacgtctt
    60421 tgccggacga gtggtggaca gtgtgaatac ccgctgggga aaattccgcc cgtttttact
    60481 ctttggtact gcgccgttaa tgatcttcag cgtgctggta ttctgggtac cgaccgactg
    60541 gagccatagc agcaaagtag tgtatgcata tttgacctac atgggcctcg ggctttgcta
    60601 cagcctggtg aatattcctt atggttcact tgctaccgcg atgacccaac aaccacaatc
    60661 ccgcgcccgt ctgggcgcgg ctcgcgggat tgccgcttca ttgacctttg tctgtctggc
    60721 atttctgata ggaccgagca ttaagaactc cagtccggaa gagatggtgt cggtatacca
    60781 tttctggacg attgtgctgg cgattgccgg aatggtgctt tacttcatct gcttcaaatc
    60841 gacgcgtgag aatgtggtac gtatcgtggc gcagccgtca ttgaagatca gtctgcaaac
    60901 cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt gcgctgtgtg tgctgatttc
    60961 gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc tatgtgttaa atgataccgg
    61021 gctgttcact gtgctggtac tggtacaaaa cctggtcggt actgtggcat cggcaccgct
    61081 ggtgccgggg atggtcgcga ggatcggtaa aaagaatacc ttcctgattg gcgctttgct
    61141 gggaacctgc ggttatctgc tgttcttttg ggtttcggtc tggtcactgc cggtggcgtt
    61201 ggttgcgttg gccatcgctt caattggtca gggcgttacc atgaccgtga tgtgggcact
    61261 ggaagctgat actgtagaat acggtgaata cctgaccggc gtgcgaatag aagggctcac
    61321 ctattcacta ttctcattta cccgtaaatg cggtcaggca atcggaggtt caattcctgc
    61381 ctttattttg ggattaagcg gatatattgc caatcaggtg caaacgccgg aagttattat
    61441 gggcatccgc acatcaattg ccttagtacc ttgcggattt atgctgctgg cattcgtcat
    61501 tatctggttt tatccgctca cggataaaaa attcaaagaa atcgtggttg aaattgataa
    61561 tcgtaaaaaa atgcagcagc aattaatcag cgatatcact aattaatatt caataaaaat
    61621 aatcagaaca tcaaaggtgc aactatgaga
//
LOCUS       DARNVM010000008        61459 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.008, whole genome shotgun sequence.
ACCESSION   DARNVM010000008 DARNVM010000000
VERSION     DARNVM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.008"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            134..805
                     /locus_tag="SI621_000486"
     CDS             134..805
                     /locus_tag="SI621_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEI4063240.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            815..2011
                     /locus_tag="SI621_000487"
     CDS             815..2011
                     /locus_tag="SI621_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEI4063241.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPS
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSATGT
                     EKLSEA"
     gene            1989..2570
                     /locus_tag="SI621_000488"
     CDS             1989..2570
                     /locus_tag="SI621_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEI4063242.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQTYLGAQVRTSNHRLDQQPVSVRTPEGIIATECDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2572..3345
                     /locus_tag="SI621_000489"
     CDS             2572..3345
                     /locus_tag="SI621_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEI4063243.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3533..3808
                     /gene="frmR"
                     /locus_tag="SI621_000490"
     CDS             3533..3808
                     /gene="frmR"
                     /locus_tag="SI621_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HEI4063244.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3843..4952
                     /gene="frmA"
                     /locus_tag="SI621_000491"
     CDS             3843..4952
                     /gene="frmA"
                     /locus_tag="SI621_000491"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HEI4063245.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5045..5878
                     /gene="fghA"
                     /locus_tag="SI621_000492"
     CDS             5045..5878
                     /gene="fghA"
                     /locus_tag="SI621_000492"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEI4063246.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6004..6543)
                     /locus_tag="SI621_000493"
     CDS             complement(6004..6543)
                     /locus_tag="SI621_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HEI4063247.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6645..7856)
                     /gene="mhpT"
                     /locus_tag="SI621_000494"
     CDS             complement(6645..7856)
                     /gene="mhpT"
                     /locus_tag="SI621_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HEI4063248.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8032..9045)
                     /gene="dmpG"
                     /locus_tag="SI621_000495"
     CDS             complement(8032..9045)
                     /gene="dmpG"
                     /locus_tag="SI621_000495"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEI4063249.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGTHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9042..9992)
                     /gene="mhpF"
                     /locus_tag="SI621_000496"
     CDS             complement(9042..9992)
                     /gene="mhpF"
                     /locus_tag="SI621_000496"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HEI4063250.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVQYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9989..10798)
                     /gene="mhpD"
                     /locus_tag="SI621_000497"
     CDS             complement(9989..10798)
                     /gene="mhpD"
                     /locus_tag="SI621_000497"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEI4063251.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10808..11674)
                     /gene="mhpC"
                     /locus_tag="SI621_000498"
     CDS             complement(10808..11674)
                     /gene="mhpC"
                     /locus_tag="SI621_000498"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HEI4063252.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11692..12636)
                     /gene="mhpB"
                     /locus_tag="SI621_000499"
     CDS             complement(11692..12636)
                     /gene="mhpB"
                     /locus_tag="SI621_000499"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEI4063253.1"
                     /translation="MYAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12638..14302)
                     /locus_tag="SI621_000500"
     CDS             complement(12638..14302)
                     /locus_tag="SI621_000500"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEI4063254.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14493..15326
                     /gene="mhpR"
                     /locus_tag="SI621_000501"
     CDS             14493..15326
                     /gene="mhpR"
                     /locus_tag="SI621_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HEI4063255.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLH
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15403..16485
                     /gene="lacI"
                     /locus_tag="SI621_000502"
     CDS             15403..16485
                     /gene="lacI"
                     /locus_tag="SI621_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HEI4063256.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16608..19682
                     /gene="lacZ"
                     /locus_tag="SI621_000503"
     CDS             16608..19682
                     /gene="lacZ"
                     /locus_tag="SI621_000503"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEI4063257.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDECWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYVEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPVLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEVPLDVAPQDKQLIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADILADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSVQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19734..20987
                     /gene="lacY"
                     /locus_tag="SI621_000504"
     CDS             19734..20987
                     /gene="lacY"
                     /locus_tag="SI621_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HEI4063258.1"
                     /translation="MYYLKNTNFWMFSLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21053..21664
                     /gene="lacA"
                     /locus_tag="SI621_000505"
     CDS             21053..21664
                     /gene="lacA"
                     /locus_tag="SI621_000505"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HEI4063259.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRNKQYYYKDYKVEPSV"
     gene            complement(21752..22933)
                     /gene="cynX"
                     /locus_tag="SI621_000506"
     CDS             complement(21752..22933)
                     /gene="cynX"
                     /locus_tag="SI621_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HEI4063260.1"
                     /translation="MRDHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLPALDHSVQPTIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMGWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22939..23409)
                     /gene="cynS"
                     /locus_tag="SI621_000507"
     CDS             complement(22939..23409)
                     /gene="cynS"
                     /locus_tag="SI621_000507"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEI4063261.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGTKLDLDEDAILLLQMIPLRGCIDNRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23440..24099)
                     /gene="cynT"
                     /locus_tag="SI621_000508"
     CDS             complement(23440..24099)
                     /gene="cynT"
                     /locus_tag="SI621_000508"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HEI4063262.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPIA
                     A"
     gene            24209..25108
                     /gene="cynR"
                     /locus_tag="SI621_000509"
     CDS             24209..25108
                     /gene="cynR"
                     /locus_tag="SI621_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HEI4063263.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGGNESR"
     gene            complement(25241..26524)
                     /gene="codA"
                     /locus_tag="SI621_000510"
     CDS             complement(25241..26524)
                     /gene="codA"
                     /locus_tag="SI621_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HEI4063264.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26514..27773)
                     /gene="codB"
                     /locus_tag="SI621_000511"
     CDS             complement(26514..27773)
                     /gene="codB"
                     /locus_tag="SI621_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEI4063265.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(28010..29896)
                     /gene="prpE"
                     /locus_tag="SI621_000512"
     CDS             complement(28010..29896)
                     /gene="prpE"
                     /locus_tag="SI621_000512"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEI4063266.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29936..31387)
                     /gene="prpD"
                     /locus_tag="SI621_000513"
     CDS             complement(29936..31387)
                     /gene="prpD"
                     /locus_tag="SI621_000513"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEI4063267.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(31421..32590)
                     /gene="prpC"
                     /locus_tag="SI621_000514"
     CDS             complement(31421..32590)
                     /gene="prpC"
                     /locus_tag="SI621_000514"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEI4063268.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRRRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32635..33525)
                     /gene="prpB"
                     /locus_tag="SI621_000515"
     CDS             complement(32635..33525)
                     /gene="prpB"
                     /locus_tag="SI621_000515"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEI4063269.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            33764..35350
                     /gene="prpR"
                     /locus_tag="SI621_000516"
     CDS             33764..35350
                     /gene="prpR"
                     /locus_tag="SI621_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HEI4063270.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(35451..35726)
                     /locus_tag="SI621_000517"
     CDS             complement(35451..35726)
                     /locus_tag="SI621_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4063271.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35873..36544
                     /locus_tag="SI621_000518"
     CDS             35873..36544
                     /locus_tag="SI621_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEI4063272.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36561..36806)
                     /locus_tag="SI621_000519"
     CDS             complement(36561..36806)
                     /locus_tag="SI621_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063273.1"
                     /translation="MAVQLFITLLNQIPLLSSLQSETLPLFGYSGRGRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(37219..38034)
                     /locus_tag="SI621_000520"
     CDS             complement(37219..38034)
                     /locus_tag="SI621_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063274.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38277..39326)
                     /gene="yahK"
                     /locus_tag="SI621_000521"
     CDS             complement(38277..39326)
                     /gene="yahK"
                     /locus_tag="SI621_000521"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HEI4063275.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQVGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39413..40369)
                     /locus_tag="SI621_000522"
     CDS             complement(39413..40369)
                     /locus_tag="SI621_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063276.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(40366..41337)
                     /locus_tag="SI621_000523"
     CDS             complement(40366..41337)
                     /locus_tag="SI621_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063277.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGGMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(41330..42814)
                     /locus_tag="SI621_000524"
     CDS             complement(41330..42814)
                     /locus_tag="SI621_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEI4063278.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSIPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42863..43846)
                     /locus_tag="SI621_000525"
     CDS             complement(42863..43846)
                     /locus_tag="SI621_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4063279.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMENLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(44100..44510)
                     /locus_tag="SI621_000526"
     CDS             complement(44100..44510)
                     /locus_tag="SI621_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEI4063280.1"
                     /translation="MTIKEKISQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQTEGCYSPEMDVIFEEYALIDVV"
     gene            complement(44808..46190)
                     /locus_tag="SI621_000527"
     CDS             complement(44808..46190)
                     /locus_tag="SI621_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEI4063281.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTISRSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(46200..47150)
                     /locus_tag="SI621_000528"
     CDS             complement(46200..47150)
                     /locus_tag="SI621_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HEI4063282.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(47246..48664)
                     /locus_tag="SI621_000529"
     CDS             complement(47246..48664)
                     /locus_tag="SI621_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEI4063283.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48664..50211)
                     /gene="fdrA"
                     /locus_tag="SI621_000530"
     CDS             complement(48664..50211)
                     /gene="fdrA"
                     /locus_tag="SI621_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEI4063284.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGPHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLKFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEAHDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(50201..51064)
                     /locus_tag="SI621_000531"
     CDS             complement(50201..51064)
                     /locus_tag="SI621_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEI4063285.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCREDIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(51104..51709)
                     /locus_tag="SI621_000532"
     CDS             complement(51104..51709)
                     /locus_tag="SI621_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEI4063286.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLMAAGA"
     gene            51967..52464
                     /locus_tag="SI621_000533"
     CDS             51967..52464
                     /locus_tag="SI621_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEI4063287.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKTGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52556..53488
                     /locus_tag="SI621_000534"
     CDS             52556..53488
                     /locus_tag="SI621_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4063288.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(53530..54618)
                     /gene="pdeL"
                     /locus_tag="SI621_000535"
     CDS             complement(53530..54618)
                     /gene="pdeL"
                     /locus_tag="SI621_000535"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HEI4063289.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(55496..57529)
                     /gene="betT"
                     /locus_tag="SI621_000536"
     CDS             complement(55496..57529)
                     /gene="betT"
                     /locus_tag="SI621_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HEI4063290.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            57658..58245
                     /gene="betI"
                     /locus_tag="SI621_000537"
     CDS             57658..58245
                     /gene="betI"
                     /locus_tag="SI621_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEI4063291.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVDGNFDETQVS
                     NAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            58259..59731
                     /gene="betB"
                     /locus_tag="SI621_000538"
     CDS             58259..59731
                     /gene="betB"
                     /locus_tag="SI621_000538"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEI4063292.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNNELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            59745..61415
                     /gene="betA"
                     /locus_tag="SI621_000539"
     CDS             59745..61415
                     /gene="betA"
                     /locus_tag="SI621_000539"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEI4063293.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSTAGYYVANGRPVRAKK"
BASE COUNT        14751 a        16638 c        15632 g        14438 t
ORIGIN      
        1 cctgaaagcc ccttattttt cagctatcag taatttttaa cacttaaaaa ttagcatctg
       61 tctgtgccag agattacgtc tctgcgggca tcgttgtgct tgtagtataa taaataaaat
      121 ggagtgtcat taagtggata aggttttaga ttcagccatc ctttcttcgg caaataaaag
      181 aaagggtatc cttgctattg gcgcacatcc tgatgatata gaattgggct gtggcgcgtc
      241 gcttgctcgt cttgcgcaaa aaggaattta tatcgcagcc gtggtgatga ctaccggcaa
      301 ctctggcaca gatggaataa tcgatcgcca tgaagaatcg cgcaacgccc taaagatatt
      361 agggtgccac caaactattc atcttaattt tgctgacacc cgcgcgcatt tacagctcaa
      421 tgatatgatt tccgccctgg aagatatcat taaaaatcaa attccttctg atgttgaaat
      481 catgcgggta tataccatgc atgatgccga ccgccatcag gatcatctcg ctgtttatca
      541 agcttcaatg gttgcctgcc gcactattcc acaaattctc ggctacgaaa ccccgagtac
      601 ctggctttca tttatgcctc aggtttttga atccgttaaa gaagaatatt tcacagtcaa
      661 acttgctgca ttaaaaaaac ataaaagcca ggaacgacgc gattatatgc gtcatgatcg
      721 cctgcgtgca gttgcacaat ttcgcgggca acaggtcaat agcgatctgg gtgaaggctt
      781 tgtaattcat aaaatgattc tttgagcaaa ttgcatgaaa acctggatat ttatatgtat
      841 ggccgtagca atattgctat ggttcctgag tacgttaagg cgtaagccca gccaaaaaaa
      901 aggttgtatt gacgccatta tacctgcgta taacgaaggc ccgagtctgg cacagtcact
      961 ggataatcta ctgcgtaacc cttatttttg ccgggtaatt tgcgttaacg acggctccac
     1021 ggacaatacc gaagcggtca tggcggaagt caaacgcaaa tggggcgacc gctttattgc
     1081 cgtcacgcaa aaaaataccg gtaaaggtgg tgcgctgatg aatggcctca attacgccac
     1141 ctgcgaccag gtttttttaa gtgatgccga cacctatgtt ccgcccgatc aagacggaat
     1201 gggctatatg cttgcagaaa tagagcgcgg tgctgatgcc gtaggcggca ttccctctac
     1261 tgcgttgaaa ggcgcgggtc tgttaccgca catccgcgcg accgtaaagt tgccgatgat
     1321 tgttatgaag cgcacgctac agcagctcct gggcggcgca ccgtttatta tcagcggtgc
     1381 ctgcgggatg ttccgtactg atgtattgcg taagttcggt ttctctgatc gtactaaagt
     1441 cgaagacctt gatctcacct ggacattggt ggcaaacggc tatcgtattc ggcaggcgaa
     1501 tcgctgcatc gtatacccac aggaatgcaa cagcccgcgt gaggaatggc gtcgctggcg
     1561 gcgttggatt gtgggctacg cggtctgtat gcgcctgcat aaaagacttt tatttagccg
     1621 cttcggtatc ttcagtatat ttcctatgct gttggttgtg ctttatggcg ttgggattta
     1681 tctcactacc tggtttaatg aattcatcac caccgggccg catggtgtgg tgttggcaat
     1741 gtttccgctt atctgggtcg gcgtagtttg tgttattggt gcttttagcg cctggtttca
     1801 tcgttgctgg ttgttggtgc ctttagcgcc gctttccgtt gtgtatgtat tattagctta
     1861 tgccatctgg attatttatg gacttattgc cttttttact ggacgcgaac ctcagcgcga
     1921 caaacccacc cgctattccg cactggtgga ggcgtcaacc gcttattccc aaccttctgc
     1981 tacaggaact gaaaaactat ctgaagctta atgttaaaga gaaaaatatt cagattgcag
     2041 accaggtaat tattgatgaa actgcaggtg aagttgttat cggcgcgaat acccgtattt
     2101 gtcacggtgc cgttattcag ggtccggtag tgattggcgc aaactgcctg ataggtaatt
     2161 atgcgtttat tcgtcctggc acaataatca gcaatggcgt aaaaattggt tttgccaccg
     2221 aaattaaaaa tgcggttatt gaagcggaag caacgattgg tccgcaatgc tttatcgccg
     2281 actcggtagt tgcaaaccag acatatttgg gcgcacaagt acgtaccagt aatcatcgtc
     2341 tggatcaaca gcccgtatct gttcgaactc cagagggaat tatcgctacc gaatgcgata
     2401 aattaggttg ttatatcggg cagcgttcac gccttggcgt acaagttatt attttgcctg
     2461 ggcgaattat ttccccgaac acgcaacttg gcccgcgcgt gattgtagaa cgtaatttac
     2521 ctaccggaac ttattcactc cgacaagaac ttatccgtac aggagattaa aatgataaaa
     2581 cggacgttat tagcggcagc aatttttagc acattgcccg cttatgccgg gttaacttcc
     2641 attaccgcgg gctacgattt taccgattat tctggcgatc atggcaaccg taatttagcg
     2701 tatgctgaac tggtggcgaa agttgaaaac gcaacgctgc tttttaatct ttcccagggg
     2761 cgtcgtgatt atgaaactga acatttcaat gccactcgcg gtcagggtgc ggtctggtat
     2821 aaatggaata actggctgac aacccgaacg ggtattgcct ttgcggataa tacgccggtc
     2881 tttgcccgcc aggattttcg tcaggatatt aacctggcac tgttgccaaa aacgcttttc
     2941 acgaccggtt atcgctacac gaaatattac gatgatgtcg aagtcgatgc ctggcaaggc
     3001 ggcgtatcac tctatactgg cccggtgatc accagctatc gctataccca ttatgactcc
     3061 agcgatgcag gtggtagtta cagcaatatg atttccgtgc gtctgaatga cccgcgcggc
     3121 actggttata cgcaactatg gctaagccgc ggaacaggtg cttacaccta tgactggacg
     3181 ccagaaacac gctacggcag catgaagagc gtcagtttgc aacgtattca accgctgact
     3241 gagcaactta atctcgggct gacggcaggt aaagtgtggt acgacacccc aaccgatgat
     3301 tataacggtc tgcaacttgc agcccatctg acctggaaat tctgattcct tctgccgccc
     3361 gctatccggg gcggccttcc ctgccgatta gccccacccc ctttcctctt tgttttccga
     3421 ccacattcac cggataaatt ttattctcca gtgttatata ctataggggg gtatgcattg
     3481 acatatagaa taccccccta tagtatattg catgcagatg atgaggtgcg aaatgcccag
     3541 tactccggaa gagaagaaaa aggtccttac tcgagttcgt cgtattcggg ggcagattga
     3601 tgctctggaa cggtcgctgg agggtgatgc cgaatgccgt gccatactcc aacagatcgc
     3661 tgccgttcgg ggcgcggcta acgggctgat ggcagaagtg cttgaaagcc atatccggga
     3721 aacgtttgac cgaaatgact gctacagccg cgaagtcagc caatccgttg acgacactat
     3781 tgaactggtt cgtgcctatc ttaaatagct gaatctatta ccatattgag gaagagcgag
     3841 agatgaaatc acgtgctgcc gttgcatttg ctcccggtaa accgctggaa atcgttgaaa
     3901 ttgacgttgc accaccgaaa aaaggtgaag tgctgattaa agtcacccat accggcgttt
     3961 gccataccga cgcatttacc ctctccggcg atgacccgga aggtgtattc ccggtagttc
     4021 tcggtcacga aggggccggc gttgtggttg aagtcggtga aggcgtaacc agcgtcaaac
     4081 ctggcgacca tgtgatcccg ctttacaccg cagagtgcgg cgagtgtgag ttctgtcgtt
     4141 ccggcaaaac caacctctgt gttgcggttc gcgaaaccca gggtaaaggc ctgatgccag
     4201 acggcaccac ccgtttttct tacaacgggc agccgcttta tcactacatg ggatgctcta
     4261 cattcagtga atacaccgtg gtcgcggaag tgtctctggc caaaattaat ccagaagcaa
     4321 accacgaaca cgtctgcctg ctgggctgtg gcgtgacgac cggtattggc gcggtacaca
     4381 acacggctaa agtccagcca ggtgattctg ttgccgtatt tggtcttggc gcgattggtc
     4441 tggcagttgt tcagggcgcg cgtcaggcga aagcgggacg gattatcgct attgatacga
     4501 acccgaagaa attcgatctg gcgcgtcgct tcggtgctac cgactgtatt aacccgaatg
     4561 actacgacaa accgattaaa gatgtcctgc tggatatcaa caaatggggt atcgaccata
     4621 cctttgaatg catcggtaac gtcaacgtga tgcgtgcggc gctggaaagt gcgcaccgcg
     4681 gctggggtca gtcggtgatc atcggggtcg cgggttccgg tcaggaaatc tccacccgtc
     4741 cattccagtt ggtcactggt cgcgtatgga aaggttccgc gtttggcggc gtgaaaggtc
     4801 gttcccagtt accgggtatg gttgaagatg cgatgaaagg tgatatcgat ctggaaccgt
     4861 ttgtcacgca taccatgagc ctggatgaaa ttaatgacgc cttcgacctg atgcatgaag
     4921 gcaaatccat tcgaaccgta attcgttact gattgcccgc aggtttaccc cgtccagttc
     4981 aggcggggtt cttaatactc tccccgggca gccgtccggg ggattaaccg tgagataatg
     5041 actgatggaa ctcattgaaa aacatgccag ctttggcggc tggcaaaatg tgtatcggca
     5101 ttattcccca tcactgaaat gtgaaatgaa tgtcggcgtc tatctcccac caaaagccgc
     5161 aagtgaaaaa ttgccggtgt tgtactggct ttcagggctg acttgcaacg agcagaattt
     5221 cattactaaa tcggggatgc agcgttacgc ggcggagcac aacattattg ttgttgcgcc
     5281 agacaccagt ccgcgaggca gtcatgtcgc agatgctgac cgttacgatc tcgggcaagg
     5341 tgccgggttt tacctgaacg caacgcaagc gccgtggaat gaacattaca aaatgtatga
     5401 ctatatccgc aacgagctgc cggatttagt gatgcaacat tttccggcaa cggccagaaa
     5461 gtctatctca ggtcattcta tgggcgggct gggtgcactg gtgctggcgt tacgtaatcc
     5521 agatgaatat gtcagcgtct cggcgttttc gcccattgtc tccccatcgc aagtaccgtg
     5581 gggacagcaa gcctttgccg catatcttgg tgaaaataaa gaggcctggt tggattacga
     5641 tccggtgagc cttatttcac aaggtcaacg tgttgcggaa atcatggttg atcaggggtt
     5701 gagtgatgat ttttacgcag aacagctgcg gactccaaat cttgaaaaga tctgccagga
     5761 gatgaatatc aaaacgttaa tccgttatca cgagggttat gatcacagct attattttgt
     5821 ctccagtttt attggcgagc atattgccta ccacgccaat aaactgaata tgcgttaata
     5881 atagtgcacg actgccggat gcggcgtgaa cgccttatcc ggcctccgga tggcgcggga
     5941 atttgtaggc atgataagac gcggcagcgt cgcatcaggc atctgcgcac gacttatcta
     6001 tgtttaccac atcaaatcat caggcacttt gaagtcggca tacggatcgt cttcatcctg
     6061 ctcttcggcg ctgagcgcgc tgtgcaacac aatactgctg gcatcacgct gagcaatttt
     6121 atcggcaacg ctggcgggaa taatggcgta ttcaccttcg ctattgttat cgaccaacaa
     6181 gcgggcaatc gccagacgac cattaatcaa ctgcgcttgc gtgagcttat cgacaaagat
     6241 tttcttaatc agattaccgt cagtgaagtt aaaaccaata tcgccattgg caatggtgat
     6301 tcggttcatt tcgataagct gcttcacctg agctttatat tctttcgcca acgccgcttg
     6361 tttttgctgc tcgctaagct gtttatcacg ctcaagctgt gcctttttgt tttcttctac
     6421 cgccgcccga gcttcacgcg cctgaacacg cgattttttc gccgttctct cgaccttcgc
     6481 cgcttttttg ctggtgacta atcctgcttt gagcaattgc tcttgtaagg taagttttgc
     6541 catctttgtt tccaaacccg ctgaataatt tttagcgagt atacctgtaa acgccgtagg
     6601 tgtattgggg gcaagtccct ttcgccccta cttaataagg cgcgtcaggc atcggcgcac
     6661 ggctgcattc gtgatctccg gctcatcaaa ataaacactg ccaggccagc aacaagaata
     6721 cccggcgcag aagcggccat tacgccaacc gtgccagtgc ctaatgccag cattttcccg
     6781 gccagtaacg gaccgctcat agcccccaga cgccctaccg ccacggctgt tcccacacct
     6841 gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg catacaaaac gctttgccca
     6901 cctgtcgcaa acaaccccgc gacgaatccc gccagcagca taccgttaaa tgacgacacc
     6961 gttcccagcg ccagcagcga agctaacatg ccgctataaa tcagtagcga catggttact
     7021 ggacgcagct tatccatcaa tgcgcccaac attaacgtcc cgcttgccgc ccccatttgc
     7081 agtgcaaaca tcacccctgc cgcctgcgat ggctggaatc cttgctccac caaaagcagc
     7141 ggtagccagt tgatcaacat gtagaccacc agcagagtga agaaataaca caaccacagc
     7201 agcagcgtcg cggttgccgt ttctggcgca aataacgcac gcagtggtgg cgcagcctgt
     7261 ttttcgccag cgaaaaccgc cgactccggc agccagcgca ttaatagtgg caccagaatc
     7321 aacggcacta caccaccgac ccaaaacacc gtttgccagg ctaagtttgc ccccgcgaaa
     7381 cccagtgtcg ccgccagcgc cgcgccaatg gggacaccgc aatacatcag gctcactgcc
     7441 gtcccacgaa aacgtggacc cgcggcttca gacgtcaggg cgatcagatt cggcaacgcc
     7501 gcccccagcc cgacaccggt catcagtcgc gcaaagacca gtgaggggaa atcccaggca
     7561 atcgccgttg ccagtgagaa caaaccaaac agcgcaactg agctaattaa aatacgctta
     7621 cgaccataac ggtccgccag cattccgcca accagcgcgc cgggtagcaa accgagtatt
     7681 ccggcgctga atatccagcc catttgcatt ttatcgagtg cgaaagcctg ggcgatgcca
     7741 cccgccgcaa tgccagccgc ctgaagatcc agtccttcca tcagagcgac caaaaaacaa
     7801 agcccgatag tcagcatcag gcgggttgaa gatgatgaag gggtacgagt cgacataaga
     7861 tgaacctcgt tgcgagtgca ggtgaatcgc ggctgataca ttgtcagccg cgtaatcaga
     7921 accatttaat gactgcctac gggcgaaata taggccggat aaggcgttta cgccgcatcc
     7981 ggcagtcgtg ctgggatggg catacaaaat gcacgttctc ggcagtcatc attatttgtt
     8041 gttgcgcaga tccagcgcca cgtcgacgat catatcctcc tggccgccaa ccatccggcg
     8101 cttgcccagc tcaacgagaa tatccaccgc gcttaagcca taacgcgccg ccgccgtttc
     8161 acagtgacgc aggaagctgg agtaaacacc ggcatatccc agcgccagcg tttcgcgatc
     8221 gactcgtacc ggacgatcct gcaacggacg caccaggtcg tcagcggcat ccattaacgc
     8281 atagagatcg gtcccatgct gccaacccag tttatccgcg gcggcaataa acacttccag
     8341 cggcgcgtta cctgcgcccg cccccattcc cgcgagactg gcatcgattc ggtcgcagcc
     8401 ctcttccacc gccgcgatag agttcgccac gccaagactc aggttatggt gagcgtgcat
     8461 gccggtttgc gtttctggtt tcagcactgc tttcagggcg cggaaacggt cacggatatc
     8521 gctcatgttc atcgcaccgc cagaatccac cacataaata caggtcgcac cgtagccttc
     8581 catcagcttt gcctgctttg cgagattctc cggcgtggtc atatggctca tcatcagaaa
     8641 accaacggtg tccattccga gctcgcgggc atactgaata tgctgggcgg aaacatcggc
     8701 ttcggtacag tgcgttgcca cacgaaccac ccgcgcgcca gcctgccagg catttttcaa
     8761 atcgtgaata gtgccgattc caggcagcaa caacgtcgcg attttggcgt gcttcaccac
     8821 atccgccgcc gcttcaatcc attcaaggtc gctatgtgtg ccgaaaccat agttaaagct
     8881 ggacccctgc aaaccgtcgc catgggccac ttcaatcgaa tccacgcggg catcgtccag
     8941 tgctttggca atctggcgga cattttccag cgaatactga tgacgaatgg cgtgcatacc
     9001 gtcacgcaac gtgacgtccg agatataaag ttttttaccg ttcatgccgc ttctcctgcc
     9061 ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca gcgcactgga agtcataatg
     9121 tcgaggttgc ccgcataggc aggcagataa tgcgctgcgc cttcgacttc cagccagacc
     9181 gccgttttca gcccggagaa ctgccccacg cctggtaaat tgaccggttt atcctgcggg
     9241 atgacttcaa actgcacgcg ctgtttcagg cgataacccg gtacgtaagc ctgcaccgcc
     9301 tcagccattt cattgattga ggcttcgata tcatcttgtg aagcttcatc gctcaataca
     9361 tacaccgtgt cacgcatcat caacggtggc tctgctgggt taagcacaat aatcgccttc
     9421 ccttttgccg cgccacccac cacttcaatg gcgcgcgaag tggtttccgt aaattcatcg
     9481 atattggcac gcgtgccagg tccggcagat ttactggcaa tagaagcgat aatttcggcg
     9541 tattgaacac gcgccacgcg tgaaactgcc gccaccattg gaatggtggc ctggccgccg
     9601 caggtgacca tgttgacgtt cagttgatcg acattcgcct cgagattaac gaccggcacg
     9661 cagtaggggc cgatggcagc aggcgtcagg tcaattaagc gaatatccgg tttcgcttcg
     9721 cgcaaagcgg catcgttttt cacatgcgca cccgcgctgg tcgcatcaaa tacaatgtcg
     9781 atatcagcaa attcaggcat gttcatcagc ccgatcaccc cttcatgggt ggtggcgacg
     9841 cccatacgtc tggcacgcgc cagaccgtcg gactgaggat caatgccgac catcaccgcc
     9901 atctccagat gctgaccgtg acgcaaaatt ttaatcatca gatcggtgcc aatgttgcca
     9961 gaaccgataa tggcgacttt acgcttactc atgacagact tccttttggg gctgcgcttg
    10021 aaaatgtcgc agcaactgaa cctatgcctt cgatatgggc ttcaaaacga tcgcccgcat
    10081 tcaccgccac catcggacct aatgccccgg taagaatgat atctccggcg cgcagcggtt
    10141 cacccaggct ggccattttg cgtgccagcc agacggcggc attaagcgga tgtcccaggc
    10201 attcgctgcc gcgcccgctg gaaacctctt cgttattacg cgtcatcttc atggcgcagt
    10261 ttttcaggtc taaccccgcc ggacgttgcg ccggaccgcc gatgacatac actccacagg
    10321 aggcgttatc tgccacggta tcgacaaact gaatcgacca gtcgcgaatg cggctcccca
    10381 ccacttccag cgccggaagt acccattcaa tggcgttata caattcgtcg aaggtgatat
    10441 cggttgcggg tagatcgcgg ttcaacacca gtgcaatctc cgcttcaatg cggggttgca
    10501 gaacacggga aaaaggaatg gtttcgttat cgccataaca catgtcggca aataatgtcc
    10561 cgaaatccgg ttgatcaacg cccagttgtt gctgcacttt cggatgcgtc agccccactt
    10621 tgcgccccac cactcgccgt ccctgagcga cgtcatgttg cacattgatg tgctgaatgg
    10681 cgtaggccgc ttcagcgtta tcgataccaa tcagatcgcg cagcggcgca atcgcttcgc
    10741 cctgctctgc ggcgcggcgt aaatccgccg ccagttgctc aagagtatgc ttcgtcatga
    10801 ccgttcctta agggcgtgcg aggaaattca gcaccagttg attgaaagcg tcggcatgtt
    10861 cccactgcgc ccagtgacca cagtcgcgga agatatgcag ttcagaaccg gcaatgccgg
    10921 acagcagacg cagacccgca tccatcggca caaagcggtc gttgcgcccc cagacaatca
    10981 gggtttgcgc tttgatttcc gccagacgtg ggccaaaatc cgggaactgt ttcggattgg
    11041 cttccaggct cttaacgaag ttttccaggt gatcgcggcg cgacagcata ttattcaggc
    11101 gcgcttcaaa cagggcgtcg gtcaaatcgc tggtatcaaa aacgaagata tccatcatca
    11161 gcttcaggtt ttcgatagtc ggctgacgat aaagctgatt cagtcgctta ataccttcgg
    11221 ttggcatcgg cgtaaacaaa ctcatgccgc ccgtaccgcc gcccatcagc accagtttgc
    11281 cgacgcgctc cggccagtta agagtgaacg ccacagaact gtggccgccc atcgagttgc
    11341 ccagcaggtg gattttggcg atatccagtt gatccaccac gcttttcagg attcgcgcat
    11401 taagatccga tcgcgaacca ctattaacga tcgaatcgct cttaccccaa cccggacaat
    11461 ccagcaggat cacccgatag cccgcctcta ccagcggatc gatattgcgg ctgaagttcg
    11521 cccagccagt agcacccggg ccggaaccat gcagcaggac aacagtttcg tcgccttgtc
    11581 cgcagtcatt aaaatggatg cgcagcgttt tacccgcttc ttctacattc agaaaacggc
    11641 tggtggcggc ttcggtttgt ggctgataac tcatcatctt ctcctgcata ttcagttctc
    11701 tgttctggcg cttaacgagc caaatccggc aatccactcc gggattgggc ggtaataacg
    11761 cccttcgcta cgccagttgc caaacgcaga aatagcggca aaagcggcga cccaggtttt
    11821 gatttcatgt gtcgactttc cggcaatggc ggaaagctct tcgttactga cggcatccag
    11881 ttcctgtatg cgtccctgct ccagcaaagt catgaactgg ttatcccaaa tcgggttgag
    11941 cggatgcaga gttctctgat cctcaacaaa cttctcagcg gcgctaatca cccgctgctg
    12001 acgcaattca cgctcagtgg cgggtaaatc tttcccgctc cccaacagac ggtcgcgcat
    12061 atgggcatcg gctttcgcca gttctggcac cggcggctga tgggaaagcc caccggaccc
    12121 aaggaacagc acgcgtttat tgagagtgct ggtgaaacgt ccaatggctt cccccaacat
    12181 gcgggtacgc tggaaaccgg gcagcggtgt ggcgacaccg ttgatgaaca caggcagaac
    12241 cggcacctta tccagcccac cgagcaggaa ctccagcggc tgggcgaacc cgtggtccac
    12301 ctgcatacag taagaaacgg caagatcgat cccgctcttc atgacggcat gcgcacaggc
    12361 ctccgccagc tccacaggca cgggcagctc tcctgccgca ctgccgaaat caccaattgc
    12421 cgtcgctcca acgcctaaac agaacggtgg catcacgtca tagaaaaagc cgttgtagtg
    12481 atctggcgca aacagcacca ccagttcagg ggagaaagcc gcaatacgct cgcgggcgct
    12541 ggcaatcacg ccattgacct catcgagcac ctcttgcgcc gggtcgacat accccaccag
    12601 cggcgagtgg gaaagacagt gaagataagc gtacatatca ggctaccttt tcgacagaaa
    12661 cgtcggcatc agggcgggtc agcgtcatca ccgacgccag tttattcagg gtcttgccca
    12721 gggtttgcgg aatggcggtg gcggcaacaa agcgatccgg gcgcatcacc accagcgaag
    12781 cattgtgttg cgcgaaccag ctacgcaggc gaccttgcgt atcgcccacg cgtagtacgc
    12841 cgtcgtggtt atcctgtgcg gtatgaattt gcacttccgg caccacctga atgaagcgtg
    12901 tgcccaacgc gcgccactgc tggatttgct cgtcgctcat cccccacagt ggattgcatc
    12961 cccagccaat taccgcgaag ttcgcgccga tcgcgttatc gagcagcgtc acgtcgccgt
    13021 tttccagcgt gactttcggc tgaataaaca tcttgccgac cggagagtgc ttcgcttcgc
    13081 cctcacgcac cagcgcaccg ccgtaatatt gcggcatcgg cttgaagcgc atttcgagga
    13141 agtagcgttt tactggcggc agataattca acagccagga aacgccgtca cgtaacgtac
    13201 cctgccagcg tttcggtgga gccagcacgt tgcccgccgt cacggacagg tcaatcatcg
    13261 ctttggcgtg atcgcgacgt tcttgttgat aggtatcgag cagcgcatcg cgggctttcc
    13321 cctggataac caacgccagt ttccatgcga ggttaaaggc gtcgcgcata ccactgttat
    13381 agccctgccc ctgccacacc ggcatgatgt gcgcggcatc gcccgccagc agtacgcgat
    13441 caatacgaaa acgttgcgcc agtcgcgcgt tgtgggtgta gacacgctgg cgaatcaatt
    13501 caacattgtc cggattaggc agcactttgc ttaacagctt gcgcatattt tgcggctcac
    13561 gcagctgttc ttcggtttct cccggcatca ccataaattc aaagcgacgt accgcatgag
    13621 gcagcgcggc agaaacatac gggcgcaccg gatcacaaca caaatagata tgcggcgtac
    13681 ttaacggatc gttggcgata tctaccacaa tccactgatt tggcgcagtt ttaccttcaa
    13741 acggcacatt cagggtgcga cggacaaaac ttgccccacc atcacaggcc accagccact
    13801 gggctttgac cgtttcccgc tgcccttctg ccgttttcag gtgcaaggtc acttcgtcat
    13861 cttgctggct gaaggcctcc agctcgcggg aaaacaggca gcgcacattc ggaaaacgcg
    13921 acaatccttc cagcatcacc gcatcgacct gaggctgaat aaaggcgtta cggcgcggcc
    13981 agccaaattc atcggtcatt ggctgaatat cagcaaaaca gcgacctttt ggggtgagaa
    14041 aacgcatcgc gtgccacggc gtagtgtgcg gcagaacatt atcgaccagg ccaaccgact
    14101 gcatggtgcg cagcgcctcg tcatcaatac caatcgcacg cgggtagtcg atcaacttat
    14161 cgagtttctc caccaccagc acgtcaatgc ccatctggcc gagatagttc gccatcatca
    14221 gcccgaccgg accggcacca gcgatcgcca cctgaacgct atggttaaca gcaggctgga
    14281 tgtcagggtg ttgtattgcc atttcagtac ctcacgactc ggacaaaatg ccgttgcgcg
    14341 cacagtacag cgcaacttat tttgttaaaa acatgtaaat gattttttat tgtgcgctca
    14401 gtataggaag ggtgttttcg gctacaatca aaacatgccc gaatgtgcac caggtgcacc
    14461 acgttgtttt aaatatataa atgtcaatta atatgcagaa caatgagcag acggaataca
    14521 aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat aaacttgatg
    14581 gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc actgtgcggc
    14641 gactgctgga gacgctgcag gaagagggat atgtccggcg tagtccctcc gatgatagtt
    14701 ttcgtttgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa cagtggattt
    14761 ctgcactggc ggccccgctg ctgggcgatc tgttgcgcga agtggtttgg ccaacagatg
    14821 tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac tacccaccgt ttcagccgct
    14881 tatcctttca ccgggcaatg gtcgggcgac ggttaccgct tctgaaaacc gcctcgggcc
    14941 tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgaa atgttagcct
    15001 cccgccccgg tgatgactat caactggcac gggaaccgtt aaagctggaa gccattctgg
    15061 cgcgcgcgcg caaagagggt tacggacaga actaccgcgg ctgggatcag gaggagaaga
    15121 tcgcctctat tgccgtaccg ctgcacagtg aacaacgggt gattggctgt ctgaatctgg
    15181 tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt ccggcgctgc
    15241 aacgggtggc aaaacagatc gaagaagggg ttgaatcgca ggctattctg gtggccggac
    15301 ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt tcgtggtatg
    15361 gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgcta
    15421 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
    15481 gccagccacg tttctgcgaa aacgcgggaa aaagtagaag cggcgatggc ggagctgaat
    15541 tacattccca accgcgtggc acaacaactg gcgggcaagc agtcgttgct gattggcgtt
    15601 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
    15661 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
    15721 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
    15781 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcttta
    15841 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
    15901 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagca
    15961 ggtccattaa gttctgtctc ggcacgtctg cgcctggcgg gctggcataa atatctcact
    16021 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagcgccat gtccggtttt
    16081 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
    16141 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
    16201 gatatctcgg tagtgggata cgacgatacc gaagacagct cgtgttatat cccgccgtta
    16261 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
    16321 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
    16381 aaaaccaccc ttccgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    16441 atgcagctgg cacgacaagt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    16501 tgtgagtcag ctcactcatt aggcacccca ggctttacac tttatgcgtc cggctcgtat
    16561 gttgtgtgga attgtgagcg gataacaatt tcacacagga tacagctatg actatgatta
    16621 cggattcact ggccgtcgta ttacaacgtc gtgactggga aaaccctggc gttacccaac
    16681 ttaatcgcct tgcggcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    16741 ccgatcgccc ttcccaacag ttgcgcagcc taaatggcga atggcgcttt gcctggtttc
    16801 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
    16861 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcctatctac accaacgtga
    16921 cctatcccat tacggtcaat ccgccttttg ttcccgcgga gaatccgaca ggttgttact
    16981 cgctcacatt taatattgat gaatgctggc tacaggaagg ccagacgcga attatttttg
    17041 atggtgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
    17101 acagccgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga aaaaaccgcc
    17161 tcgcggtgat ggtgctgcgc tggagtgacg gcagttatgt ggaagatcag gatatgtggc
    17221 ggatgagcgg cattttccgt gacgtctcgt tgctgcacaa accgaccaca caaatcagcg
    17281 atttccatgt tgccactcgc tttaatgatg atttcagccg cgcggtactg gaggcagaag
    17341 ttcagatgtg cggcgaactg cgcgatgagc tgcgggtgac ggtttctttg tggcagggtg
    17401 aaacgcaggt cgccagcggc accacgcctt tcggcggtga aattatcgat gagcgtggtg
    17461 gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg tggagcgccg
    17521 aaatcccgaa tctctatcgt gcagtggttg aactgcacac cgccgacggc acgctgattg
    17581 aagcagaagc ctgcgatgtc ggtttccgcg aagtgcggat tgaaaatggt ctcctgctgc
    17641 tgaacggcaa gccggtgctg attcgcggcg ttaaccgtca cgagcatcat cctctgcatg
    17701 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
    17761 ttaacgccgt gcgctgttcg cattatccga atcatccgct gtggtacacg ttgtgcgacc
    17821 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
    17881 atcgtctgac cgatgatccg cgctggctac cagcgatgag cgaacgcgta acgcgaatgg
    17941 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
    18001 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
    18061 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
    18121 cgcgcgtgga tgaagaccag cccttcccgg cggtgccgaa atggtccatc aaaaaatggc
    18181 tttcgctgcc tggagagacg cgcccgctga tcctttgcga atatgcccac gcgatgggta
    18241 acagtcttgg cggcttcgct aaatactggc aggcgtttcg tcagtacccc cgtttacagg
    18301 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
    18361 cgtggtcggc ttacggcggt gattttggcg atacgccaaa cgatcgccag ttctgcatga
    18421 acggtctggt ctttgccgac cgcacgccgc atccggcgct gacggaagca aaacaccagc
    18481 agcagttttt ccagttccgt ttatccgggc gaaccatcga agtgaccagc gaatacctgt
    18541 tccgtcatag cgataacgag ctcctgcact ggtcggtggc gttggatggc aagccgctgg
    18601 caagcggtga agtgcctctg gatgttgctc cacaagataa acagttgatt gaactgcctg
    18661 aactaccgca gccggaaagc accggacaac tctggcttac ggtacacgta gtgcaaccga
    18721 acgcgaccgc atggtcagaa gccggacaca ttagcgcctg gcagcagtgg cgtctggcgg
    18781 aaaacctcag cgtgacactc ccctccgcgc cccacgccat cccgcatctg accaccagcg
    18841 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
    18901 ttctttcaca gatgtggatt ggcgatgaaa aacaactgct gacgccgctg cgcgatcagt
    18961 tcacccgcgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
    19021 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagcg gcgttgttgc
    19081 agtgcacggc agatatactt gccgacgcgg tgctgattac gaccgctcac gcgtggcagc
    19141 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtgttcaaa
    19201 tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggca cggattggcc
    19261 tgacctgcca gctggcgcag gtcgcagagc gggtaaactg gctcggatta gggccgcaag
    19321 aaaactatcc cgaccgcctt actgcggcct gttttgaccg ctgggatctg ccattgtcag
    19381 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
    19441 tgaattatgg cccacaccag tggcgcggcg acttccagtt caatatcagt cgctacagcc
    19501 aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
    19561 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtgt
    19621 cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
    19681 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
    19741 atttaaaaaa cacaaacttt tggatgttca gtttattctt tttcttttac ttttttatca
    19801 tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa
    19861 gtgatacggg tattattttt gctgctattt ctctgttctc gctattattc caaccgctgt
    19921 ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt attaccggca
    19981 tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactgtta caatacaaca
    20041 ttttagtagg atcgattgtt ggtggtattt atcttggctt ttgttttaac gccggtgcgc
    20101 cagcagtaga ggcatttatc gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg
    20161 cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc atcatgttca
    20221 ccatcaataa tcagtttgtt ttctggctgg gttctggctg tgcactcatc ctcgccgttt
    20281 tgctcttttt cgccaaaacg gatgcgccct cttccgccac ggttgccaat gcggtaggtg
    20341 ccaaccattc ggcatttagc cttaagctgg cgctggaact gttcagacag ccaaaactgt
    20401 ggtttttgtc actgtatgtt atcggcgttt cctgcaccta cgatgttttt gaccaacagt
    20461 ttgctaattt ctttacttcg ttctttgcca caggtgaaca gggtacgcgg gtatttggct
    20521 acgtaacgac aatgggcgaa ttacttaacg cctcgattat gttcttcgcg ccgctgatca
    20581 ttaatcgcat cggtgggaaa aacgccctgc tgctggctgg cactattatg tctgtacgta
    20641 ttattggctc atcgttcgcc acctcagcgc tggaagtggt tattctgaaa acgctgcata
    20701 tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc cagtttgaag
    20761 tgcgtttttc agcgacgatt tatctggtct gtttctgctt ctttaagcaa ctggcgatga
    20821 tttttatgtc tgtactggcg ggcaatatgt atgaaagcat cggtttccag ggcgcttatc
    20881 tggtgctggg tctggtagcg ttgggcttca ccttaatttc cgtgttcacg cttagcggcc
    20941 ccggtccgct ttctctactg cgtcgtcagg tgaatgaagt cgcttaagca attaatgtcg
    21001 gatgcggcgc gagcgcctta tccgaccaac atatcaggac ggaatgatcg caatgaacat
    21061 gccaatgacc gaaagaataa aagcaggcaa gctatttacc gatatgtgcg aaggcttacc
    21121 ggaaaaaaga cttcgtggga aaacgttaat gtatgagttt aatcactcgc atccatcaga
    21181 agttgaaaaa agagaaagcc tgcttaaaga aatgtttgcc acggtagggg aaaacgcctg
    21241 gatagaacct cccgtctatt tctcttacgg ttccaacatc catataggcc gcaattttta
    21301 tgcaaatttt aatttaacca ttgtcgatga ctacacggta acaatcggtg ataacgtact
    21361 gattgctccc aatgttactc tttccgttac gggacaccct gtacaccatg aattgagaaa
    21421 aaacggtgag atgtactctt ttccgataac gattggcaat aacgtctgga tcggaagtca
    21481 tgtggttatt aatccaggcg tcaccatcgg ggataattct gttattggcg cgggtagtgt
    21541 cgtcacaaaa gacattccac caaacgtcgt ggccgctggc gttccttgtc gggttattcg
    21601 cgaaataaac gaccggaata agcaatatta ttacaaagat tataaagttg agccttcagt
    21661 ttaaataagc actgaaataa atgtcggatg cgccactggg cacatccgac aataatttac
    21721 tcttccatca tctggcgaaa caagcgtcgc atcatgcctc tttgacccac agctgcggaa
    21781 aacgtgctgg tgcaaaacgc agggttatga tcatcagccc aacgacgcac agcgcatgaa
    21841 atgcccagcc catcaggtaa ttgccgctga tactacgcag cacgccagaa aaccacgggg
    21901 caagcccggc gatgataaaa ccgatcccct gcataaacgc caccagcttg ccagcaatag
    21961 tcggttgcac agagtgatcg agagccggca gcaaacagag cggaaatgcg ccacccagac
    22021 ctaacccaca caccatcgcc cacaataccg gcaattgcag cggcagccag ataaagccgc
    22081 agaaccccac cagttgtaac gccagcgcca gcatcaaaag tttgcgccga tcctgatggc
    22141 gagccatagc aggcatcagc aatgcccctg cggcttgccc aagcgtcatc aatgccagca
    22201 aggaaccgct gtactgcgcg ctggcaccaa tctcaatata gaaagcgggt aaccaggcaa
    22261 tcaggctggc gtaaccgccg ttaatcagac cgaagtaaac acccagcgtc cacgcgcggg
    22321 gagtaaatac cacgcgaacc ggagtggttg ttgtcttgtg agaagaggcg acctcgcggg
    22381 cgctttgcca ccaccaggca aagagcgcaa caacggcagg cagcgcccac caggcgagtg
    22441 tttgatacca ggtttcgcta tgttgaacta accagggcgt aatggcggca ccaagtccac
    22501 cgccgcccat cagagccgcg gaccacagcc ccatcaccag tggcgtacgg tgctgaaacc
    22561 gccgcttaat taccgaaggc atcactgcct gaatgatgcc gatccccact ccaccaagca
    22621 gtgcgctgct aagcagcagc gcactttggg ggtaaagctc acgcatcaat gcaccgacgg
    22681 caatcagtaa caaactgatg gcgacgctgc gacgttcgct gacatgctga tgaagccagc
    22741 ttccggccag cgccagcccg cccatggtaa ccaccggcag agcggtcaac agggctgcca
    22801 cgctaaagct cattccactc gcctgacgca attgcggtag cagtggtccg acggaggtga
    22861 gcagtggtcg catattaaga ccaatcagca ccagaaccag cagcatcgtg aacgtctttt
    22921 gatggtcgcg catggttgtc agaacggttt ggtcggcaga tatttgccat ctaaggtgat
    22981 gaccgcgcgt tcgccacctt ccggatccgc cactttctta acgtcgagtt tgaagttaat
    23041 cgcgctgata atgccatcgc caaacttctc atgaaccaac gctttcaggg ttgtgccgta
    23101 cacctgcaac atttcataga aacgatacat cgttgggtcg gttggaatac ggttatcaat
    23161 gcagccacgc agtggaatca tctgcaacag tagaatggcg tcttcgtcga gatccagctt
    23221 cgttccgacc aggcgggcgg cgtcggcagg aagcgcctgc tgccccagca aagccgcggt
    23281 tacaaaggct tctgccagac cggtgccgtc ggcaatctcg gcaaatgaga gatctttttt
    23341 agctttgctg agcaaaatgg catcggcaag atcaagacga atattgcggt taatttgcga
    23401 ctgaatcatg gtggatctcc tgatggttta aaaataaggt tacgctgcga tcggttggcg
    23461 tagcggtatg gcacaaacgc gaggattagc ggccagtggc acaaactggc gggttgcgcc
    23521 gtcaaaagct gcgatgctgc cgctttcaat gtcgtagacc cagccgtgca gggcgatccg
    23581 cccctcttcg agcgccaggc gcaccgatgg atgagtttgc aaattagcca actgagcaat
    23641 gacgttttca cgtaccatcg ccgcagcttt tgacggtaaa tcgggatgcg ggcgcgcctc
    23701 attaacgacg cgggctgaat cggcataacg cagccagtga gagacggcag gcatatggtc
    23761 catgcactgg cagctggcaa tagcggtcat tgcgccacag ttggaatgac cgcaaatcac
    23821 aatgtcagat acccgaagcg cagcgacggc atactccacc gaagcagaaa cgccaccggg
    23881 ttccggcccg taggaaggga cgatattgcc cgcgttgcga ataacgaaca gatcgccagg
    23941 ctcacgttgc gtcaccagct cagggaccag acggctgtcg gagcaggaga taaaaagtgt
    24001 gcgcgggctt tgctgtgtcg ccagctgttt aaacaaggct tcccgcttcg gaaatgcctc
    24061 gcgctggaat ttaaggaatc catcaataat ctctttcacc gttaacctct gtctgtctct
    24121 ggaatgagag gcagactact cgccgattgt cataaggtaa aagtctcatt tatgatgagt
    24181 tctattggat ttacttatag gttggcgaat gctctctcga catatcaatt actttcttgc
    24241 cgtggctgaa catggcagct tcacccgtgc cgccagtgcg ttgcacgtct cccaacctgc
    24301 gctttcccag cagattcgcc agttagagga gagtttaggc gtgccgctgt ttgaccgtag
    24361 cgggcgaacg attcgtctca ctgatgcagg agaagtctgg cgacagtacg ccagccgggc
    24421 gttacaggaa ctgggggcgg gtaaacgggc gattcatgat gttgccgatc tgacgcgagg
    24481 atcgctgcgt atcgccgtca ctcccacctt tacaagctac tttatcggcc ctttaatggc
    24541 ggatttctat gcgcgctacc ccggcatcac gctccagcta caggaaatgt cgcaggagaa
    24601 aatcgaagat ctgctttgtc gcgacgagct ggacgttggg attgccttcg cgcctgtgca
    24661 ttcgccggag ctggaggcaa ttcctttact gacagaaagt ttagcgttag tcgtggcgca
    24721 acatcatccg ctggctgcct gtgaacaggt ggcgttgagt cgcttgcatg atgaaaaact
    24781 ggtcctgctc agcgcggaat ttgccaccag agagcaaatt gaccactact gcgagaaagc
    24841 ggggctacat ccacaggtgg tcattgaggc gaactcaatt agcgcggttc tggagctgat
    24901 tcgccgcact tccctttcca cattgttacc agcagcgatt gccacacaac atgacgggct
    24961 taaagcgatt tctcttgccc cgccactact ggagagaacg gcggttttgt tgcggcggaa
    25021 aaatagctgg cagactgccg ctgcgaaggc atttttgcac atggcgttgg aggaatgtgc
    25081 ggatgttggc ggaaatgaat cgcggtaacc actgacacaa ttgcctgatg cgctacgctt
    25141 atcaggccta cgagttcagt gctctacgta ggccggataa ggcgttcacg ccgcatccgg
    25201 cataaactac gctcgctttg tcaacacaca gcccagccga tcaccgtttg taatcgatgg
    25261 cttctggctg ctccagatat acggtggttt gtgccggttg tgtgctggca atcaccttgc
    25321 cgccacgtac cgaataacgt accggaacct gacggcgcag cgcatcaaac ccattttcag
    25381 ccggcaggat aatcaggttg gcgctgtttc cggcggcaat gccataatcc tgcaaattca
    25441 acgtccttgc gctgtggtgg gtgattaaat tcaggccatc gttaatctgc ccgtagccca
    25501 tcaactggca aacatgcagc cccatatgca gcacttgcag catattcgcc gttcccagcg
    25561 gataccacgg atcgaagaca tcatcgtgac caaagcagac gttaatgccg gactccagca
    25621 tctctttaac gcgcgtgatg ccgcgacgtt ttggatacgt atcgaaacgt ccttgcagat
    25681 gaatattgac cagcgggttg gcgacaaagt taataccgga cattttcagc aagcggaaca
    25741 ggcgtgaggt atacgccccg ttataggagt gcattgccgt ggtgtggctg gcggtgactc
    25801 gcgcgcccat gccttcacgg tgcgccaggg ctgcaacggt ttcgacaaag cgcgactgtt
    25861 cgtcatcaat ctcatcacag tgaacgtcga tgagacggtc gtatttttgc gccagggcga
    25921 aggttttatg cagcgactcc acgccgtatt cacgggtaaa ttcaaaatgc ggaatcgccc
    25981 ccactacgtc tgcccctaag cgtaacgcct cttccagcaa cgcttcaccg ttgggatacg
    26041 acaaaatccc ttcctgcggg aaggcgacga tttgcagatc aatccacggc gcgacttcct
    26101 gcttcacttc cagcattgct ttcagcgcag tcagcgttgc atccgaaaca tcgacatggg
    26161 tacgcacatg ctgaatgccg ttggcaatct gccatttcag cgtttgccag gcgcgttgtt
    26221 tcacatcgtc atgggttaat aacgctttgc gctcggccca gcgttcaatg ccttcaaaca
    26281 gcgtgccgga ctgattccag ttcggttgtc cggcggtttg cgtggtgtcc aggtgaatat
    26341 gtggctccac aaacggcggt ataactaaac cttgttcggc atccaggctg ttttcagtta
    26401 tgggcatcac cccggattgc gcatcaatgg cgctgatttt tccgtccagc agatgaatct
    26461 gccacagccc ctctttgccg ggtaaccggg cgttaataat tgtctgtaaa gcgttattcg
    26521 acactgttag cctccacatg cgtcattgca gccgtcgttt tgcgattcaa aatcgggtta
    26581 aggatcagat agctcagcgc gccacctaat accgcgttga ccggaacaat tcccggtaac
    26641 cagtggcctg cggcaatccc caaggcgacc gccagaatcg ccacccaatt gacactcatc
    26701 atacgcgtgg tcgcaaagtg ctcatagcgg cgacggttca tcagatagtc agcgatgatc
    26761 acgcccccta ccggaggaat agctgccgaa aggaaggtca gccagccgac aaaattgtta
    26821 tacaaccata atgcgcagac cgtaccgata ataccgttaa tcaccgaaag ggttttactc
    26881 gacataccgg taatgttggc gaaacctaaa cccgacgcat aaagtgcgtt atcgttggtg
    26941 gtccagatat tcagccccag caccacaatc gcaggcagca gcagaccctg agcaatcatc
    27001 acatcagaga tatccgccat ccccagtgcc gccgcgcccg ctgcaccgaa aataaacatc
    27061 aacgagttgc cgaggaaaaa ggccaccatc gccactagca ccgccagttt ggcattacga
    27121 ccaaaccgga caaagtcagc ggtgagcgtg cccgcactga taaatgaccc cacaaccagc
    27181 gccagcgcga cattgaaatc taacggttgt gcgggaacga ccgcttttaa tgcgtccagg
    27241 ccgcccatgc cgttaacggc caaccacacg gaataaccgc caagacaggc gatagccgga
    27301 actgcaatca ccgaaagaac cgttagcgcc gaaatgccaa agaagacggt gacggtcatc
    27361 agtaaaccgg aaacggcaat cagcaaatta atatccagcc cagttgcctt gcccaccgga
    27421 atggcaaaca tcgccacgcc aacgccaaac cagccaacct gggtgccgcc cagcagcagt
    27481 gaaggcagcc atgagccttt aacaccaaaa gagaagcgag caagaagatg agtggtcagg
    27541 ccggtttttg cgccaatgta accaagaaat gaagtgtaaa taccgaggag aagattaccg
    27601 atgaggactg cgaggaagaa atcatgatag ctaagaccgg ttccgagagt gccgccggtc
    27661 cacatactgg cggaaaagaa ggttaatccc agcatgacga acgttaatgc caatacccct
    27721 ttccgcgccg actgcgggac tggcccctgg ctaaagttgt tatcttgcga cacgaaattc
    27781 cccctttttg ctgtttgaaa cccaaaaaat ccgccgcatt ctattcatct gaagataaaa
    27841 agcaatcgtt ttcgtgggga aatatatttt ttatatgaat gagtgcaaaa aggtggggga
    27901 aaaattatcg gtgtaaaaac agtgggtagc cataataaga cgcggtaagt gtcgcatcag
    27961 gcatcggcgc atggtgtcgg atgcgccaga cgacgcatcc gacaataacc tactcttcca
    28021 tcgcctggcg gatctgatcc aacgacgcag gatcatcaat ggtcgtcaga tccccaggat
    28081 cgcgtccttc acaaatcgcc tggatcgtgc ggcgcagcat ttttccggat cgcgttttcg
    28141 gcaattgcga gacaaaccag acgtgcgccg ggcgaccaaa gttgccaatc tggctgtcca
    28201 ccagcgccat aatcgccttc tcttgcgagt gcgccacctc acggtcttcc agactgtcgc
    28261 tctctttcgg aatgacaaac gccaccgcca cctgcccttt cagcgcatct ttcaccccaa
    28321 ccaccgccac ttcggcaacg cccggatgac tggagatact ctcttcaatc tcgcgcgtcc
    28381 ccagccgatg cccggcaacg ttaatcacat cgtcagtgcg cccgagaata aagtgataac
    28441 cgtcagcgtc acggatgccc cagtcaaaag tggcgtacac cggacgggaa aacagcgacc
    28501 agtacgtctt cacaaagcgg tcgtcgtcgc cccaaatggt ctgaatacag cccggcggca
    28561 gcggcccttc taccaccagc atccctttct cgttgacgcc acacggttcg ccggtgactt
    28621 cattaagcaa ctgcacgtta tagccataca tcggcacgcc ggggcttccc agacgcgtcg
    28681 gcctgtcgtc cagaccgcga gcaatcgcca taatcggcca gccggattcg gtctgccagt
    28741 agttgtcgat gaccggcaca tccagcgtat tgctcaccca actggcggtc ggctcgtcca
    28801 gcggttctcc agccagatag agcacttcca gcgacgagag atcgtgtttg cgaatttcag
    28861 cggtagggaa ttttttcagc acgcgaatgg cggtcggcgc tgagaacatg cggctaacct
    28921 gatatttctc gacgattttc caccacacgc cgcagtccgg ccaggtcggt aatccttcgt
    28981 aaacgatagt cgccatcccc gccagcagcg gcgcgtagac gatatacgaa tgccccacca
    29041 cccagccgat atccgaagca caaaagaaca caccgcccgc tttgccgcca aaaatggtgt
    29101 ccatcgaggt cgccagcgcc accgcatatc cgccaacgtc acgctgcacg cctttaggtt
    29161 tgccggtcgt gccggaggtg tagagaatgc aggaggtttc gttggattcc agccacgcca
    29221 ctggcacccg cgcgccgatg tgttgatggc gcaacgacgc gaaatcgaca tcccgcccgc
    29281 tgacgcgcgc cattttcgcc agcccacgat ccaccagcaa aacatggcgt ggctggtgct
    29341 gcgcctgact tatcgcatcg tcgagcaatt ttttatacgg aatgattttg ccaccgcgcg
    29401 ctccggcatc agccgagaca atcagcaccg gtttagcgtc atcaattcgc gccgccacgc
    29461 tgtgcgaggc aaatccccca aacaccaccg agtgaatagc gccaatgcgc gcgcaagcca
    29521 gcagagtaat atgtgcttcg gcaatcatcg gcatatacac cagcacccga tcgccgcgct
    29581 gcacacccag cgagcgcagc attgaggcca ccgcattcac ttcgtcatgt aactggcgga
    29641 aggtaaaggt gcgctcttct tctgtttccg aagagacggc aatcagcgcc agcgcctctg
    29701 gctgtttctc cagccagcgg tcgatagcgt tgtggcacaa gttggttcgg ccttcacaaa
    29761 accaacgggc aaacggcggg ttgctgtggt cgagcgtttg cgtaaagggc gtctgccagt
    29821 caatacgccg ggcctgctcg gcccagaact gctccggttc gttaatcgaa cgctgataaa
    29881 attcgctaaa agacataatg ctctcctgtt gaacttacgc cttaccgccg tttacttaaa
    29941 tgacgtacag gtcgagatac tcattgaccg gcatctgttc caggcgagct ctgtcgagag
    30001 aaacctccag aatgcgctgt tgctggcgag tcgggaactg gcgcgcgaga ttgattttga
    30061 atttatcgac cagtttcgga atgccatcct gacggcggcg agcatgacca atggggtact
    30121 ccaccaccac ttcttcaaat cgcgtaccgt cggtgaactc aagggttatg gcattggcga
    30181 tggcacgttt ttccgggtcg tggtagtcag cggtaaatgc cggatcttca aagcaattga
    30241 tcttctcgcg cagggcgtcg atgcgtttat cttgcgcaac gttgtcctcg taatctgccg
    30301 ccgttaagcg tccgaacagc agcgggatcg ccaccatgta ctgaatgcag tggtcgcggt
    30361 ctgccgggtt attgagcggt ccttttttgt cgatgatgcg aatacaggct tcgtgggtgc
    30421 gaatggtcac tttttcgata tctgccgccg ttttgcctgc tgcctgcatc tgttcataga
    30481 gcgtcatcgc tgcttcaact gccgtctggg agtggaactc cgccgggaag gagattttga
    30541 acagcacatt ttccatgacg taagaaccgt acgggcgctg gaagcggaac gattcacctt
    30601 tgaaggagac gtcgtagaag ccccacacag gcgcagtcag ggctgacggg taacccattt
    30661 cgcccgtttt cgccatcagt gccagacgta ccgcgcggga agtggcatcg cccgccgccc
    30721 aggatttacg cgtgccggtg ttcggcgcat ggcgataggt gcgcagcgac tgaccgtcca
    30781 cccacgccag cgaaacggcg ttgagaattt cctcgcgggt caggccgagc atttcggcga
    30841 ccacggcggt ggaagccact ttcactaaca gaacgtggtc gaggccgacg cggttaaagg
    30901 agttttccag cgcgatgcag ccctgaattt catgggcttt gatcattccg gtcagcacct
    30961 gcttcatggt caacggcgct ttgccgctag cgaccgcgtt gcgcgaaagc cagtccgccg
    31021 ttgccagaat gccgccgagg ttgtcggaag gatgccccca ctccgccgcc agccaggtat
    31081 cgttgaaatc gagccaacgg atcatcgcgc cgatgttaaa tgccgcctgg acggggtcga
    31141 gctggaactg agttccgggg acgcgcacgc cgttagggac gacagtgcca ggaacaattg
    31201 gccccagcag ttttttacag gccgggtatt cgagagcttc cagaccgcag ccgagagtgt
    31261 cgagcaggca gtaatgtgcg gtgtcgtagg ccactttaga gctgatttcg tagttcatca
    31321 cgtaatcgac gatatcaacg atttcacgat caaattccgg gcggatgttg ttgatttgag
    31381 ctgacattgg gtacgtttcc ttattgttat tcgtagaggt ttactggcgc ttatccagcg
    31441 cgacaaactg gcggtcttcc ggtccaacat aattggcgga aggacggata attttgttgt
    31501 cctgacgttg ttcgataatg tgcgccgccc agccggtgac gcgggcgata acaaacagtg
    31561 gtgtgaacat ctcggtggga acgcccatca tgttgtagga aacagcggag aaccagtcga
    31621 gattggggaa catctttttg ctctcccaca tcaccgtttc aaggcgatcg gcgatgttgt
    31681 acatcttcag cgaaccgcct tcctgcgaga gctgcttcgc cacgcgtttg atcacctgat
    31741 gacgcgggtc ggcgatggtg taaaccggat gaccaaaacc aatgaccact tctttgtttt
    31801 ccacgcgcct gcggatatcg gcttcggctt cgtccggtgt ttcgtagcgt tgctggatct
    31861 ccagcgacac ttcattcgcc ccgccgtgtt ttggcccgcg cagtgcgcca atcgcgccaa
    31921 taatcgcgga atacatatca gagccagtgc ccgcaatcac ccggctggta aaagtggagg
    31981 cgttaaactc gtgctcggcg tacagcacca gcgaaatatg catcgccttt tcccagcttt
    32041 gcgacggctt ttcgccatgc agcagatgca ggaagtgacc gccgatggaa tcgtcatcgg
    32101 tttctggctg gatgcgttcg ccgttgtggc tgtagtgata ccagtagaga agaatcgagc
    32161 taagcgacgc caacagtttg tcggcaatat cccgcgcacc agagacggta tgaccctctt
    32221 tttctggcag cgtgcaaccg agcgcggaga cgccggtgcg cataacatcc atcgggtgcg
    32281 acgccgcggg taaggcttcc agcacggtac gcacgttagc tggtaaaccg cgcagggctt
    32341 tcagtttcgt tttgtaggcg gcgagttcgt cacgggtcgg cagtttgccg tggatcagca
    32401 ggtgcgccac ttcttcaaat tcgcaatgtt ccgccagatc aagaatatcg tagccgcggt
    32461 aatgcaggtc gttgccgctt ttacccacgg tgcagagcgc cgtattgccc gccggaacgc
    32521 cggatagtgc cacagatttt ttcggtttaa tgacatgggt actgttttgc aggatcgttg
    32581 tgtcgctcat gttgtcctcg tcatttgtag ggtcgagata tttttcgttg tgttttattt
    32641 cgcctggcta cgggcaaaca ggtcgtcgag cttctcttcg tactggtagt agttgatgct
    32701 ttcgtacagc tcgttgcggg tttgcatggt gtcgatgaca cttttctgcg tgccttcctg
    32761 acgcaggacg ttgtagacat gttcggcggc gcggttcatg gcgcgaaacg ctgaaagcgg
    32821 gtacagcgcc attgcaacat gggcgctgcg taattcgtcg gtggtaaaca gcggtgttgc
    32881 gccaaattcg gtgatgttgg cgaggatcgg cacctgcacc gcatcggcaa actggcggta
    32941 catggcgagt tcggtaatcg cctccgggaa caacatctcg gcacccgctt caacataggc
    33001 ctgcgcacgc tcgatcgccg catccagccc ctctaccgcc agcgcatcgg tgcgcgccat
    33061 gatcacaaaa tcaggatcgg ttttcgcatc caccgccgcg cggatccgat ccaccatctc
    33121 ctctttcgag acgatcgctt tgttcggacg atgaccgcag cgtttcgcac caacctgatc
    33181 ttcaatatgc agtcctgccg caccggcttt aatcatcgat ttcacggtgc gcgccacgtt
    33241 aaaagcagaa gaaccaaaac cgatatccgc atccaccagc agcggcagcg aacaaacgtc
    33301 ggtgatacgg cgaatatctg tcagcacgtc atcaagggta gaaataccga gatccggcag
    33361 ccccagcgaa cctgccgcca cgccaccgcc ggaaagataa atcgcctgat atccggcacg
    33421 ctgcgccagc agcgcatggt tggcgttaat ggtgccaaca atctgcaatg gattttcttt
    33481 agtcagtgca gcgcgaaacg ctttacctgg agagtgtaga gacatagccc atcctttgtt
    33541 atcaacttgt tatttgcgtt gataaacaca aagcaagggg tgtgccacaa ttgccttaat
    33601 aaaattaaat gtttaatttc aatttgttag aaatatagtc atgaagtaat ccgcaaatat
    33661 gcgtttcagt taacgtttca ggcaatgttt caccgcgttt cattgcaaca attatgaaac
    33721 aagactaaac ccaatattcg gtttcttaac tttgcggtgc gctatggcac atccaccacg
    33781 gctgaatgac gacaaaccgg ttatctggac ggtatctgta acgcgcctgt tcgagctgtt
    33841 tcgcgatatc agcctcgagt ttgatcatct ggcgaacatt acccctatcc agcttggctt
    33901 tgaaaaagcg gtgacctaca tccgcaagaa actggccagc gaacgctgcg acgccatcat
    33961 cgctgctggc tctaacggcg cgtacctgaa aagccgcctg tcagtgccag ttattttgat
    34021 taaaccgagc ggctacgatg tgttacaggc actggcaaaa gccggaaaac tcacctcttc
    34081 tatcggcgtt gtcacttatc aggaaactat tccggcgctg gtggcgtttc aaaaaacctt
    34141 taatttgcgc ctcgaccaac gtagctacat taccgaagaa gacgcgcgcg ggcagattaa
    34201 cgagctgaaa gctaacggca ccgaagcggt ggtcggcgcg gggctgatta ccgatttggc
    34261 agaagaagcc ggaatgaccg gaatttttat ctattccgcc gccaccgtgc gccaggcatt
    34321 cagcgatgcg ctggatatga cgcgcatgtc gttacgccat aacactcacg atgccacccg
    34381 taacgccctg cgtactcgtt acgtgctggg cgatatgctc ggtcaatcac cacagatgga
    34441 acaggtgcgg cagactattt tgctgtatgc ccgctccagt gcggcggtgt tgattgaggg
    34501 ggaaacgggg acgggcaaag agctggcggc ccaggcgatt catcgggaat attttgcccg
    34561 ccacgatgcg cgacagggca aaaagtcgca tccgtttgtt gccgtcaact gcggggcgat
    34621 tgccgaatcg ttgctggaag cagagctgtt tggctatgag gaaggggcgt tcaccggctc
    34681 gcgacgcgga ggtcgcgccg ggctgttcga aatcgcccac ggtggcacgc tgtttcttga
    34741 cgagataggc gaaatgccgc taccgttgca gacccggctg ctgcgggtgc tggaagaaaa
    34801 agaggtcacc cgcgtcggcg ggcatcagcc tgttccggtg gatgtgcgag tcattagcgc
    34861 cactcactgc aatctggaag aagatatgcg gcaagggcag tttcgccgtg atctgtttta
    34921 tcggctgagt attttgcgtc tgcaattgcc accactgcgc gagcgggtgg cggatattct
    34981 gccgctggcg gaaagctttt tgaaagtgtc tctggcggcg ctctccgccc cgttttctgc
    35041 cgcattacgc caggggttac aggcaagcga aaccgtgctg gtacactacg actggccggg
    35101 caatattcgt gaactgcgca atatgatgga gcgactggcg ctgtttttaa gtgtggaacc
    35161 gacgccggat ttaacgccgc aatttttgca gctgctactg ccggaactag cgcgcgagtc
    35221 agcgaaaatt cccgctccac gcttactgac accacaacag gcactggaga aatttaaagg
    35281 cgataaaaca gcagcggcga attatttagg cataagccgg acgacgttct ggcggcggct
    35341 gaaaaactga actgccgaat gcagcgcgaa cgctttatcc ggcataaatt tcaatagatg
    35401 atgatcaccg ataaacttac gcatcgggga tgtttacata ggtttcagaa ttacttcttc
    35461 ttataaatat ttgccgtgcc gtgaatctta ttgtcagttt gaccagaggt cagcaccaac
    35521 acatcagccc ctttttcatc ggcttttttg attaaatctt cttttgcatc tgcagtcgac
    35581 atttcattac tggttgaaat atcacctatt ttttcatatt gcgattcaac tttttcaaat
    35641 tccgctttgg tcatcaattc agcggcatag acattggtaa cggcaaacgc taacgcaccg
    35701 actaacattt tagagattat tttcatatta cggctccaga gttgttatca cactatcacc
    35761 caccgacatt attgggttga ttaataatta gttcagtcag ggcataaacg ccagtaaccg
    35821 gaaataagac actttgtgat ctctctcgca cccgattatt tttggtagaa atatgatgca
    35881 gttagttcac ttatttatgg atgaaatcac tatggatcct ttgcatgccg tttacctgac
    35941 cgtaggactg ttcgtgatta ctttttttaa tccgggagcc aatctctttg tggtagtaca
    36001 aaccagcctg gcttccggtc gacgcgcagg ggtgctgacc gggctgggcg tggcgctggg
    36061 cgatgcattt tattccgggt tgggtttgtt tggtcttgca acgctaatta cgcagtgtga
    36121 ggagattttt tcgcttatca gaatcgtcgg cggcgcttat ctcttatggt ttgcgtggtg
    36181 cagcatgcgc cgccagtcaa caccgcaaat gagcacacta caacaaccga ttaacgcccc
    36241 ctggtatgtc ttttttcgcc gcggattaat taccgatctc tctaacccgc aaaccgtttt
    36301 attttttatc agtattttct cagtaacatt aaatgccgaa acaccaacat gggcacgttt
    36361 aatggcctgg gcggggattg tgctcgcatc aattatctgg cgagtctttc ttagtcaggc
    36421 gttttctttg cccgcagtgc gtcgtgctta tgggcgtatg caacgcgttg ccagtcgggt
    36481 tattggtgca attattggtg tattcgcgct acgcctgatt tacgaagggg tgacgcagcg
    36541 gtgagcagat aatttactct ctacgtaatc aacctgattt gccgaatccc gaccatcggc
    36601 gatgatagcc cccgtaatgc gtggtcctta tttccgataa gtttacttga gtctttagct
    36661 tcccatttca gcccactctc ccctgtgtgg gcttttttca ttgctctgcc ccgtcctgaa
    36721 tagccaaaca atggcaatgt ttcactttgc agggatgata ataaggggat ctgattcaga
    36781 agagttatga aaagttggac cgccatcgag gcctcgaacc ccgtaccata caacaatcct
    36841 ggttgtcgtc tgctcttcct gatgagctaa tggcggtttt tgatatgagc cttacagcat
    36901 attttctgtc aggcttgagt gcgatgcgta agatacagaa aagaccccac acaaacatga
    36961 tggaattatg atttttttca tatgaatgtc aacccacaca ttcacaaaac atctgcatga
    37021 gatcattttt tatacaccgt atagctgcga aaccactgct gatgcttttt taagcgaagc
    37081 gtcgccgatc acaacaaaat atcggcgaaa aaggcggtgg aatgccattt ggcatacgga
    37141 tatacagatg aaatgattac ctgctttgcg attcactctc tggtggaata acaactcgca
    37201 ggtggaaccc tttctgaact aaagagtgta gcggatttta acgtgcttat ttacaggata
    37261 atatctgtca cctaattcct gcactctatt agatatccat tgatttaatc gcgcatcatc
    37321 tctgtatgtt tttacgtcca gaacagagag caataacatc accagtgatt caggaaaaac
    37381 agggtagata ttttttaagt gtaataataa tccatgcgca agcgcctgct taatgggtgc
    37441 gtcggtctcc ggaatagacg acatttcgcg aagccatttt tccacatgca tacactcttt
    37501 ccctttaggg aaaagtctca gcaaaggcat atggtgttca agaagaatac atttgcaatc
    37561 tgttcgcact gaatcgataa actgcactaa tttcttaagc ccggtaaagc gtccagcctg
    37621 cacgtttgct ttaatatcaa gaaacgcatc gtcctggtca tatacccaaa ctgtcccttt
    37681 ttgattttca agtatgcgaa aaacaaactt aaccgggtga tcagggtaat ccatccagtc
    37741 ataaaaccca gggaaatcat taaactgcac atttatggtg agtgcgttat cgtctgacca
    37801 ctcacagtct cgtacggcag ccatgagatg aatgagtttg tcgtcatcga ctaaatcatc
    37861 acgacaatca taaggataat cataatattg tttaatcaaa accttgaata tatcgacaca
    37921 ggagaccagg gagcttttga tatcctctgg taactgattg ttttttactg tattaagtat
    37981 gctttgttgc gtatatggca tcaaattatt gtgcggagct tttaatgata tcatcgcgat
    38041 ctctcttaaa taaaaataaa cgtcgcagat aataaagccc tgattaaacc aggcactatc
    38101 agaaatcgct cataactaac gtgtgaagta ttgtgtactg gagggagtta atttaacaat
    38161 ttgaatttac gttataggtt ttactgctac tttgatatta tttcgctctc cttaactaaa
    38221 aagttaagtg gagagcgaag ttaatatgtt aaaccacagg gtatttatta attttttcag
    38281 tctgttagtg tgcgattatc gataacaaaa cgatatttca catctcctcg cagcattcgc
    38341 tcataggctt cattaatttg atcggcccga atcatctcta tatcagccac gatgccatgt
    38401 tcggcgcaaa aatcgagcat ctcctgagtt tctggaatgc cgccaatcat agaaccggct
    38461 atcgcacggc gtttcatgat caggttgaaa acttccggcg atttatgcgg tgtcgcaggc
    38521 gcaccaacca gcgtcatggt gccatcacgc ttcagcaagg tggtaaaatc gtcgagatta
    38581 tgtggcgcag ctactgtatt caaaatgaaa tcgaaactct tcacatgagc cgccatctca
    38641 tcggcattgc gtgagttaac aacttcatcg gcccccaggg cttttgccgc ttcgcgtttt
    38701 gactcagaag tggtaaatgc caccacatgc gcccccatcg cgtgggccag cttaatcccc
    38761 atatgtccca gaccgccgat gccgaccaca cccacttttt tacccggacc gacctgccag
    38821 tgacgtagcg gcgaatacgt ggtgatccct gcacacaaca aaggtgccac cgccgccagc
    38881 tgctcttgcg ggtgacgaat acgcagaaca tatcgctcat gaacgacgat ctgttgtgag
    38941 tagccgccca gagtatggcc cggttcgtcc ggcgtcggcg agttataggt gccggtcatg
    39001 tgatcacagt agttttccag cccgtcttca cactcttcgc aatgtttaca actgtcgaca
    39061 atacagccga caccgaccag atcgcccggc gcatattttt ctacctgatc accaacggct
    39121 accacgcgcc ctacaatttc atggcccggc acgcagggat aaaccgtccc cgcccactcg
    39181 gaacggacct ggtggagatc ggaatggcag acgccacagt aagcgatttc aattttgaca
    39241 tcatgcggtc ccggttcacg ccgggtgata tccatcggtt caagtggttg tttagcggaa
    39301 tatgcaccaa cagctttgat cttcattgtg tttactcctg attagctatg tgtatttggc
    39361 gatgcctgat atgtcatcgc ggcaaaacgc gtccattgaa tatagccaat atttaaacca
    39421 cccgatccag ccccaacatg ccgcgagcct gaatgaaaca gagcagcaac gttccccata
    39481 aggccatcgt caggtagggg cttataccga gaatattgaa gccgctttca ataatttgca
    39541 gaagaaacag cgccagcacc ataccaatta tccgcccgct gccgccgtcc ggattaaccc
    39601 cgccgagtac tgccgcgagg attgacacca gcaagtacga ttcgccataa gaggccttag
    39661 ccgagttgag tttcgacatc atcaaaaatg ccgctaccgc acaaagcaag gcggaaatga
    39721 cataaaccca aatcagcacc cgacgagtgt taatgccgga gtaaagtgtt gctcgttcgt
    39781 tagaaccaat cagataaatg gatttaccga gcggcgtttt ctccagcaat atccacagcc
    39841 ccagcgccac tgcggcaaac aaccacatcg gtagcggaat accgaaccac tgtgcatgat
    39901 tcaaccacag cacccatgag ggataattgg caatggcgct accgcctgtc accagaatat
    39961 ttaccccttt gagcaacgtc atcatcccga gtgttgcaag aattggcgac actcgaatac
    40021 cagcaatcaa tacgccgtta cataaaccga ttatcaccgc cgcgccagcg cccgccagca
    40081 gcgtagcgac aaccgtagca atacccggtg gatactgagt cgccacccac gccatcacca
    40141 gtgaacaggc gttagcagtg gctataattg acaagttaat gccaccgcaa agcatggtca
    40201 ccgccatagc caacgccaga atgcccagca cgggcatttg cgaagcgacg gactggaaat
    40261 tacttatcga ccagaaaatc tccggcatag taatgctgaa cgcggcaaca gttagcacca
    40321 gcaggccgat caagtaaaac tcaacgttat tacgccacga ttttttcata gcagactccg
    40381 gttctgatgc tgactccacg ccgtggcgct aatgctggca acgatgatga tgccggtgat
    40441 caatgtttgc cagtaagacg agactcccaa taaatttagc ccgttttgca tcactgccaa
    40501 caacaccacg ccgagcaaag taccggttaa cgtgccgcgc ccgccgagca aactagtgcc
    40561 gccaagcacc accgcagcca gtactgtcag ctcataaccc agaagagaat cgggggcgac
    40621 agtcatcacc gtccacgact gcactacacc cgcagcgcca gacatcaatc ccatatagcc
    40681 gtagacgaaa agttgcagtt tcagcacgct aaagccgatg cgactggcgg attcacggtt
    40741 cccgccaagg gcataaattt tgcgccctac actggtgaaa ttcatgataa acgctgtcag
    40801 cagcaccacc gtaatcatgg cgatcagcgg cagaccaagg ccatagtcat agccgtcagc
    40861 atcggtgtac ttgaacaaca ttcccccctg ctcaaaccac ggcggaaagt cgtacagcca
    40921 cacaccttta ctcaaccata acagcaggcc atagaaaata ttcagcgtcg aaatggtgat
    40981 gatgatagac ggcacccgta ggcgattaac cagtaaggcg ttaattaagc caagtaaaat
    41041 gccgatgccg cccgccagtg caaacgcaac agcaaagttg ccaccaattt tttgcagcag
    41101 cagcaccatg ccgtattgcg caatgatggt cattgccgga aacgaaatat caatcccacc
    41161 ggaaattaac accacaaaca aaccacaggc gagaatagtc aacatggcat agttattggc
    41221 gagatcgtac aaattaccga atgtcagaaa ctcgtcgctg cgccaggcaa ggccaacaaa
    41281 cagcaccaca atcagtaatg ccagccagaa ttcatggcgt tttttcagtt cagccattta
    41341 ccacctccgc aatcctggcc tgagaactgc tgtcaggcag gaagctatgg gtgatctgac
    41401 ctttttgcat taccaaaatc cggtggcttt gataccacgc ttcttcaatt tcatcgcaga
    41461 tcatcaatac cgcaatgcca tgagccgcca gatcgctgat aatgccgtat attcctgctt
    41521 tgttggcgat atcaacaccg acagtcgggc tatcgagaat caacagtcgt ggcccaatcg
    41581 ccagccattt ggcgatcgat actcgctgcg cgttaccccc agaaagcgta ttcaccggga
    41641 gatgtgggtc agagactttg atggtcaatg ccttgattag cctgtccacc aggtcacgca
    41701 ctttagcttc actcagaaag ccgaagcgat cttttacctt gtgaaaaaca gtgctaatga
    41761 tgttgtcttc aatagattgc gccatcacca aaccgcgcga catgcgatct tccgaaacat
    41821 aaccaatacc gtggcggatg gcgtcctggt tactatgcag tgtaaccagc tggccgttga
    41881 tgaggatctc gccagcatcc ggtcgggtca taccaaacag actcagacat aattccgtgc
    41941 gccccgcacc aagcaggcca acaatcgaca ccacttcgcc cgcttccacc cgcaagttaa
    42001 tattttgata ttccccatga cgacttaaat tacgcacctc cagcactggg atgctggatt
    42061 ttccctgcca caactctcgc acctgatatt caaactcctg gccggtcatc aggaaaccga
    42121 gttgttttgt ggtcatctct gcagccggaa aggtcccgac cagctcacca tctttcagca
    42181 ctgaaatacg gtcggaaact tccatcactt cttccagacg atgactgacg aagaccacgc
    42241 agatcccgcg ttcccgcaat tgatgcacca cctgtaataa cccttgcact tcctgatggg
    42301 tcagcgaagc ggttggttca tccatcacga tcaaccgtgc gtcctgcgcc agtgcacggc
    42361 aaattgccac cagctgacag cgggcaattg aaagctcaga aacgagcgta tccagtggca
    42421 atgtgacgtt aatactggtc attgccgcct gcgccacctc acgcagacga cggcggttaa
    42481 caaacagacc gtggtgatag tgattcacgg cgatattctc ccagacactc aggttaggaa
    42541 ataaagagag gtcctgataa atcacctgaa tcccctgcgc caccgaagcg gcgggtgtca
    42601 gctttgacca gtttttccca ccaagcgtaa tttccgctcc ctcatccggg cgataaacac
    42661 cggacataat tttaatcaac gtcgatttac cacagccgtt ttgcccggca aggcagtgca
    42721 cctcacccga cataaagtcg agagaaacat cgcgcagcgc tcgcgtggca tgaaacgttt
    42781 tgttgatatg acgaagggaa aggaaggttt ccatagcatc ccctggcaat aagaatcggg
    42841 gctttcgccc cgaagaaatg gattaataaa gtgagtcgat attgtctttg ttgaccagta
    42901 ataccttatg gaaacggata attcgcttat ccatatctac gtcggcctta ccgagattct
    42961 ccatttctaa tccaggtttg atctcttcac ccttcagcaa cgtgctggcg acggccgcga
    43021 gtgcgtaacc tgcagaggcc ggatcgtaag taatcccttc ggtgatatca ccacttttaa
    43081 tcagtgatgc cgcctgtgaa gggatcatca tgccatagac tgcgacttta tttttcgccc
    43141 gtttctcttt cactgcacgt cccgcgccaa tcggaccgtt tgaaccaaag gagacaaccg
    43201 ctttcaagtc aggataggtt ttcatcaggt ccagtgtggt acgacgtgag tcatccacac
    43261 tctcggcaac cggcatgcgg cgggtaactt catgcatatc cgggtaatgc tctttctggt
    43321 atttcaccag caagtcagcc cataagttat gctgcggcac ggtcaaacta cccacgtaaa
    43381 tcacatagcc gcccttgcca cccatgcgtt tcgccatatg ctcaacatat tcagcggcaa
    43441 atttttcgtt atcaatgatt tcgatatccc agttagcact tggctgaccg ggggattcgt
    43501 tggtcagaac cacaattccg gcatctcgcg cttttttgaa taccggttcc agcacgttgg
    43561 catcgtttgg cacaattgta attgcattaa ccttacgggc gattaaatcc tcaatgattt
    43621 taacctgttg cggagcatca gtacttgaag gccccacctg tgaggcatta acaccaaagg
    43681 ctttacccgc ctcgaccaca ccttcgccca tgcgattaaa ccacggcata ccatcgactt
    43741 tagagatatt caccacgact ttttccgctg cctggagcgg cgcagaaatt agcgcagcgc
    43801 ctaataacag cgaagacacc atattgataa caaaacgttt attcatcatt tcagcctcat
    43861 tgcagggtag tcaggaagtg ttcgcccaaa ttagcgcgct ggagagaata agaattttcg
    43921 aatattttta tgcagaataa acagccctat ttcaggcagc tattaaaaat ttggttataa
    43981 atatggacaa aacgggcagt aacaataagt gtgacaatca tcacgtaaaa aacagcaaaa
    44041 taaacaaaat tagcgatact ttatgtttgt gagcttattt cgcgtagaaa aatagttatc
    44101 tacacaacgt caataagggc atattcttca aagataacgt ccatttctgg tgaatagcat
    44161 ccttctgtct ggaaaaaacg acggtgttca ttacgccaat attcaagact gaggtcacct
    44221 tcaccttcct tacgggcaag ctctgcggtg acatcagaaa agcgcagtaa atgcaggcca
    44281 atcactctga taacacagac aggctcacca cggctatttt ctactatctt atactcacca
    44341 atcatcggaa atgcgttgtc ttcaatacat cccgccagcg aaccacagga agctgtttta
    44401 acacctgtcg caatcaacgt tgcgaggtga tcggccaacg ctgctgaatc gccaaaagta
    44461 caaaaggaag catggggata tttttgtgag attttttctt ttatggtcat gagaatattt
    44521 tcgcgattac ggaaaaaaat cacatcttaa ttactgttga taaccatcag caagaggatc
    44581 attggttttt ctatgtttaa tcttttttat ccgaagccgg tcacatttat ggcttgataa
    44641 atacctttgt taaaactcac ttttgtgtat ttctctcaaa taatcccctg gaaattatgc
    44701 cgggagatta taaagttcag gatcattcat tagcaaacca ttgctgcccc actccctggg
    44761 gctttaaatc tcttacgatt caccattgca ggcgtttcaa atgcgtatca acctgccaca
    44821 ctcccccaca ccagttgccc tttatggaac gtcgccgttc tcggcgagat acgcgccacc
    44881 gcctcggcgg aacaggaggc gtccaccagc acaaagctgg catcatcctg cgctttgggc
    44941 cacacgcgct cgcctttttc gttgagcggc aatacatcgc cagtggctaa aaacagcgag
    45001 cgggagaggt tctgttcgtt aggacgaata tagagctgcg cgtacagatt ggctttttcc
    45061 agcatgtcgc ccaggccgta aggcgaccag tggtcgataa cgctgtcggt gccggtcatc
    45121 acttttacgc ctttgtcgtg caactgtttg agcggcatat gcagcgtgcc aatcggcacc
    45181 gtcgaggcga tagaaatttg ctgcgccgcc atccggtgcg ccagttcatc tacctgttgc
    45241 tcattgagcg ttgccagcgc aaaggcgtgg ctgatggtca gcttgccctt cagttgcggc
    45301 gttttctcca ccgtttcaac catataattg atggctgcca cgcctgccgg agtggtttcg
    45361 tgcaggtgaa tatcaacgcc tttgtcgtag tccagcgcaa tctggaacat ggtgtcgagg
    45421 gatttttcca tcgcgccatc aacactggtc gggtccagcc cgccgacgta atgcgccccc
    45481 gcctgcatcg cttcgcgcat taagggttct gatttcgaca gcagcaaacc gtgctgcggg
    45541 aaggcgacaa tttcacactc gaagcccgcc tgacgtcgcg ccagaaccgc ctgcaaattt
    45601 tgcagatttt tcaggccgga aaccggttcg atattgcagt ggctgcggga aatggtagtg
    45661 cctttcgact gcaataaatc aatcagtttt tccgcccttt cctgagtgta cggttgcaac
    45721 tccggcagca ttttctgctc gagtttgatc atgtcctgga tggtggtgcc tgccggacga
    45781 ttgagcgagc gccacggccc gccgtagaag gttttgtcga gatgaatatg catgtcgcgg
    45841 gtggtgggca gcatcagctt accgccagcg tcatagtgcg gcagcgtggc gtccggatgc
    45901 tgattgttct cgcgcagtgc aacaattttg ccgttctgaa tttccacagt ctggcgcgcc
    45961 gtgcgggtct gcaccgccac gccattttcg tagtcaaaac cggtttccag caacacgttg
    46021 tcgagatagt agtgcgggtc agtgattttc atggtgttat tcgcgtcgca gtgggttgtg
    46081 ccagagactg ccgcatgggc gagcggcaca gaggtaccaa acagcgcggc ggcggtgacc
    46141 atcttaccgc tctggctcag aaactcacgg cggctattgc tttctttcat cttacgtcct
    46201 tacgttttaa taatatgggt gcccgtttca ccgcgcagcg ccgcaggcag gcattccggc
    46261 gtggtgataa tcacttcttt gccgccctgt tccaggaacg tcaggctggc gatgattttt
    46321 ggcaacatgc tgccaggcgg aaaatgccct tcctgcatat agcgggtcat ggtggcaata
    46381 tccacccgat cgagcgcctg ctgctgcggt ttgccaaagt gaatacacac tttttcgacg
    46441 ccagtggtga tcaccagaat gtcggcgtga atttcacggg ccaatagcgc ggtagagaga
    46501 tctttgtcga taaccgcgtc cacgctttgg taatcccccg cttcactgca caccaccgga
    46561 attccgccac cgcccgcgcc aatcaccaca aagccttgtt ggatcagcgc cttaatggca
    46621 ggtgcttcta caatacgttg cggttccggc gaggcgacca cgcggcgata gccccgtccg
    46681 gcatcttcaa caaaacgcca gtcagggttt gccttttgta attcgtcacg ctggctgtca
    46741 ctaaagaatg cgccgatggg cttggtggga tgagcaaaac ccggatcgtt tttatccact
    46801 tccacctgag tcaccacggt gacggctttt ttctcgccat gacgcgccag tcggttgttc
    46861 agcgcctgtt ggatcaagta gccaatgccg ccttgcgtat ccgccacaca gttcgccagc
    46921 ggcgttaagg gcagcccttc gcgctcgtgg gcaatctccg cgcggcgtaa atccaaaccg
    46981 acctgcggtc cgttgccgtg ggtcagcaca atgtcgtaat cggaagccag catttccagc
    47041 accgtatcgg cgacggcttt caccgcctcc gcctgatgct caatcgactg gctggcgtta
    47101 tctttgataa tgctgttgcc accaatggca acgaccacaa gttctttcat attgttatgt
    47161 ccgctacaga gggtgatggg gttatgagag tgaggttgcc cctctcccca gaaggaagaa
    47221 gggatcgtaa ggtgcagttg gagggtcaga cgccgtaacg ttcgcaccag ccaagaatgg
    47281 ctttctcgaa gcaggcgaat ggtggatgca caatgcccgc gccaatcatg ccaacgcccg
    47341 catctttatg ggcaatggcg gtgttgatca ccggcagaat gccgctactg cccacgcggg
    47401 tgatgtcgat tgccgacggc acgcccataa aaccgagcag cggaatggtg acgttggggt
    47461 tttcaccgag agtgatttcg cgcatctgac gggagaaatc gatagcctct tccaccgtgc
    47521 cgcccaccag cgcgacgatc gcgggcgcgg tagccatagc aaatccacca atgccgtagg
    47581 tttcggtgat ggcgctgtcg ccaatatcca gccccgaatc ttccggctta taaccggcaa
    47641 acatcgggcc gatcacctgc tgcgccggac cggtaaacca ttgccccggt aatccgctga
    47701 cccgcaggcc gaactcgacg ccgttacgcg ccatcgtggt gaccacggtg ctgtattcga
    47761 tgccgtgcgc cgcatccatc gccgctttac acatcgccat ccacgtcggg ccggagaagt
    47821 agtcgctgct ggcgacaaac tcaaacactt cgcgctgttg ctcgacggaa taacctgcct
    47881 gaataatccc cggcgtcagc gcctgaatca gcagcgtcgt cccggcgtta ttacggttat
    47941 ggcattcatc gcccatatgc agcgcctgcg ccagcattaa gcgcagatcg atttcgccga
    48001 taattttcat cgcgtcgcgc agcattggcc cctgcacatc gcgcatccag ttgaggcggt
    48061 caatcacgct ctggtcgtta gcgcccatac gcaaaatctt cgccatctgc tcgctcatat
    48121 tggtgtaagc gatgttgccg taggttttgt tttttacgat gtgcataaac atcgaggccg
    48181 aggtaacacc cgccatcgat cccacacagt cgtgttcatg gcacggcgag aaggtgatct
    48241 ccccggaggc cgccaagtca gccgcctcgt cgagatcttt cgccagccct tcgaacacca
    48301 gcgccccggt gaccgcgcct ttcattgcgc cgcacatttt ttcccaggtc actggcggcc
    48361 cggcgtgaag aatggttttc ggcgtcattc ccggcaccac gttaatcgcc tgatcaaaac
    48421 caatcagcac cggatgcgac tggataatgc gctccagcgc ctgctggtta gcggcggcga
    48481 ttttgtctgc cagcggcgaa tcggcaatgt tatccagcgc ctgcaccacc tgcatattgc
    48541 cctgccccgg cggcgtccag tcgagttggg tcacttctac atgctgcttt ttcaaatcat
    48601 cgctaaacat ggcgatgccg acgttaataa cgttcaacgg ttggctaaac agtgactggc
    48661 tcatcaggct tcctcccctt tgcagataaa ttcacgcgcc agcaatccgg tattggtgct
    48721 gctgcttgcc agaattaccc cagcatcaag cagcatctgg ctttgttgtt ctaacgatgg
    48781 cgtatcgaga tcggtaccca gcacataggc gagaatgatc aactcgcgcc cttcggcagc
    48841 ggcgatcgct ttcgcctctt tgatcgcctc gatggtggaa ccgaccggat cttcatgcga
    48901 tccaaacccg agcacaaaat ccatcacgat caccgccact tctggatcgt gcgcctcttc
    48961 gatcaagcgg ctgatacggt tggtggggtc aatcattgga tgcggcttgc cgttggtgaa
    49021 gtcgtcatcg ccaaagtcga ggaaggtgtg tttgatgctg cggttgatat cttgcaggcg
    49081 gaattcagga tcgggctgaa tgttgctgta gacatcgcca tgtttttcca tcaccgcgaa
    49141 catggtttcg tcacacagcg taccgccgca gaacaggcca cgaatgtatt tctgttgcgg
    49201 cttcaggcga gcgcgcacgt cagcaatcaa tggctggttc agggtatgca gatcgagatg
    49261 ttcctgtttc acgccggaga gcatcaccgc tttcagcgcc gcttctttgc tgccgcgggc
    49321 aaactttagc ccctgctcat ccactggcgt ttcgccacga ccgaggaagc agaccaccac
    49381 cggcttgcgg caggcgcgtg cgcgttccag cactttgcgg gccaccgcag gcgcaggcgg
    49441 tttggagata agcacaataa tttcagtttg cggatcgttt tccagcatcc cgatggcgtc
    49501 gagcatcatc aggccgccga ttttctcgct caggtcgcgc ccgccggtgc cgatcagttg
    49561 cgaaacgccg ccgccaaatt catggatacg gacgctcagc tcctggctac cggtgccgga
    49621 tgcgccaaca atgccgatgt tgccgcgacg cacggcgtta ccaaagcaga gcgccgcgcc
    49681 gttgataatc gccgtaccgc agtctggccc catcatcagc agcccttttt cgtgggccaa
    49741 ctgtttgagc gccagttcat cttcaactga gacgttatcg gaaaacagca tcacgttaag
    49801 atcgttttgc agcgcctgac gcgcttcgcg agcggcaaac agaccgttga ccgaaatcac
    49861 cgccaggtta ctttccggga tatgcttttt ggcgctggca atagtggcgt aacgcgcctc
    49921 gtgcgggccg ctttgcgctt tgctgttgaa cagttcttca atctccacca gcaactgctc
    49981 gttgtctgcg cccgatttac cattgatgac aatcatcagg tcaccgtttt tcgcctgctc
    50041 cagctccggc gtcagcagcc ctaaattctt cagcacgcct ttgttcattt cggtcgccat
    50101 cgccacaaat gcctgctcga cgccgtcaag tttatttgca cgcgtggaga tagacatcag
    50161 cgagacagaa tcaaaatagg tattcggttt aatgactatt ttaactgaca taattccctc
    50221 cgtaggtttg gctcagcgca caaccatcgg ccatgccata cagcatgtcg cagccggaac
    50281 tggagccaat atgtttaatt ttttctattg cctgagttgc gttcccaggg atgtcataaa
    50341 taatgttatg aataaaacga tgaatatctt cccggcagcg ttgttgtaat gcggcttcca
    50401 gcgttatggc acttaataac gtggtattat ttctgccgcg ttgcagaccg agataaaatt
    50461 cctctttgta tttttccgcc ggatgcccag gaataaataa aataagcgcc agacctgtta
    50521 aataatcgtc ggctgatggc gtaaggccaa tccctaagcc catcatactg gcgaccgctg
    50581 aggcgatatt ttgtttatcg tttacggcct gaataactgc ccgtcgttta atatgtaatt
    50641 ctttatttaa cgcctgataa aacggattat cgccgcaata attaaacagg gtttcatttt
    50701 cccccaggtg ctgatgaata atgtcatgcc actgttgcca ggcgataagg tgaaaattca
    50761 cagcggtcat ttgcaaggtt ggggacagcc aacgccgaca acgactcatc tctatatgaa
    50821 gatgttgacc aaccgtaatg ccttgatcgt caaactgaac cttatcacca tgccggaaca
    50881 gaccatcaaa gtgagtgagt gccaaccgac aactgtttgg cgcattgtcg tactcttcac
    50941 aaagcagcgt cagcaactgc tggcgggccg gaagtgcgat atttaccgct ctggcaaaga
    51001 cctgttcaac ctgtccttgc ccccgctgcg ccagaaaatc agcatccgca gttagcgccc
    51061 acacacaccg tctttctggt gtgttgatag caagcctccc ggtttatgca cctgcggcca
    51121 tcagtaactg cgcaatctct tcaaagcctc gttcccgcgc cagttccagc ggcgttttgc
    51181 catatttatc cgtcatgtgc gggctggcac cgtgttccag caataactgg acaatcgcct
    51241 gctgtttaat cccgccatca ttaagcacaa ttgcttccag tagcggcgtc cagccaacat
    51301 ggttggtctg gttaacgtta atctccgtat gcgccaaaag ctcttttaca atacttaaat
    51361 ggcctttttc acaggcaggc gtcaggccga caccgccaaa acgggtgacg caattaagat
    51421 cgggtttagc cggtaaaata atccgtaaca gggttaaatc gccggtcagg cagctaatta
    51481 aaaaaggatt tgaacatatt tgatcttgct tattaatatc tgctccggca tcaattaatg
    51541 cctgaacgca agcatattgc tgatataaac ttgccagcgt aattgccgtt ttcccctgac
    51601 gatcgcaggt attaatatcg acacccagcg caaggcaggt ttttacttta tcgatatcac
    51661 cctgttgcgc agccaataaa tagtcggcag gtagattttt tatagtcatt cacattcccc
    51721 tggtttttgt ggctgttttc gatgaattga gaatagcaac gataatctga taaaagaacc
    51781 cgcttaaatt taattcgccg tatttcgcct ttttgttgaa tgatgttcaa caatcttttc
    51841 gcagtgtgcg gtagtgcaat atttgtttct ttgacccgat accctttcac tcaatcaggt
    51901 tttatttctc tatagtctgc acatgtaaga gtagctttcg tccatgtcta accagagggg
    51961 aacaagatga acggactcac ggcaacgggt gtcaccgtcg gtatttgtgc agggctttgg
    52021 cagttagtgt cttcacatgt tgggctctct cagggatggg aattattagg aaccattggc
    52081 ttcgtcgcct tttgcagttt ttatgccgca ggcggcggta aaacggggtt tatcagaagc
    52141 ctggcggtta attattccgg gatggtgtgg gcgtttttcg ccgcgctggc tgccggttgg
    52201 ctggcctcgg tgagtggatt gtccacgttc tgggcgagcg tgattatgac tgtacccttc
    52261 tcggcggtag tggtctggca aggacgtttc ggtttattgt cttttattcc cggaggcttt
    52321 ctcggcatga cgctattttt tgccagcggg atgaactgga cggtgacatt actgggtttt
    52381 ctggcgggta actgcgtggg gataatttca gaatatggcg gacaaaaatt aagcgaagcg
    52441 acgactaaac gcgacgggta ttaaaagtta ttcaaaaatg attatctatc tttaaccgat
    52501 acctctacag tcatggctgg agaaattcat agtagataat ggcgggaatg gtttaatgaa
    52561 ctcaattttt accgaagaga atctgttggc atttactacc gctgcgcgtt ttggcagctt
    52621 cagtaaagcc gcagaagagt tgggtttaac cacttccgcc attagctaca ccatcaagcg
    52681 aatggaaaca gggctggatg tggtgctgtt cactcgcagt acccgaagca ttgagttaac
    52741 ggagtccggg cgttattttt tccgcaaagc taccgacctg ctgaatgatt ttcacgccat
    52801 caaacgcagc atcgatacta tttcgcaggg tatagaagca cgggtgcgca tttgtattaa
    52861 ccaactgctt tatacgccca aacacaccgc acgcttatta caggtactga aaaagcagtt
    52921 tcctacctgc cagattaccg tcaccaccga agtgtataac ggcgtctggg atgcgattat
    52981 taataatcag gcgaatatcg ctatcggcgc acccgacacg ctgctggacg gtggcggcat
    53041 tgattacacg gagattggcg cgattcgctg ggcttttgcc atcgccccgg atcacccgtt
    53101 agcctttgtg ccggagccga tcgccgaaag ccaactgcgt ctctacccta atatcatggt
    53161 ggaggacacc gcacatacga ttaataaaaa agtgggctgg ttgctgcatg ggcaggagtc
    53221 aattctggtg ccagatttca acaccaaatg tcagtgccag attttaggtg aaggaattgg
    53281 ttttttgccg gattacatgg tccgtgaggc gatggcgcaa tccctgctgg ttacccgaca
    53341 aatccataat ccgcgccagg attcgcgcat gttactggcg acccagcact ctgccaccgg
    53401 tcaggtgacg cagtggatta aaaaacaatt ttcgccaaat ggcatattaa cggggatcta
    53461 tcaggattta ctgcatcggg agaattaatc gctgccaact tcccgatgcg ctgtgaatag
    53521 cggtttacat caaccacctg ctttcattac ccattcagag ataaatttat tacccggtac
    53581 tggcggagag tacaaatagc cctgcaaaaa gtgaacgcct ttgccgatca ttaaatccgc
    53641 ctgctcctgg gtttctaccc cttccgccac gatactcaga ccaggcttac gcgccagttc
    53701 gacaatattg tccacaatat gaccggatat ttcgtccacg ctcgccattt gcacaaatga
    53761 cttatcgatc ttaataaaat cgaccgggaa cgcctgcaag taacgatagg tcgcataacc
    53821 cgtaccaaag tcatccagcg caaaggtaat gttgtgctga tgaaggctgt caaatatcgc
    53881 tctggcttct ggcgttaccg gaatagggtt acgttccgtt agctcgagaa ccagtttgat
    53941 tttatcgtta cctaatttct taaccaggtt caggcattct ttttcaaatc ccgcagccaa
    54001 aaaacaaccc gccgagacgt tgatgccaat atggaaattg tccggcagca aatgttttac
    54061 cggcatcaga atatccgcag tctgtttcat caactggcga gtcattatga cgataagacc
    54121 agatgactcc gccagaggaa taaactgatc cggtgggata attcccgttt gtggatgttc
    54181 ccagcggaca agcacctcac agcccgtcag taccccagtc tgcgcgcaga aaaccggttg
    54241 gatccacggt ttaaattcat ggttttccaa cgccagactg atggcttcag gcgtgacgat
    54301 atggtgatag tgattatcat taatatattt gtgactatta ttacccgtgg cggatatgat
    54361 ctccggattg tactgaaaga aaatatcccg ccagaaagta atatcgctct gaatccccag
    54421 tttctcaaag agctgtttct tttgatgtga aatggtcttt gcactgcgat ttctgtactg
    54481 tgatatttct gttacagaca ttccctgcat cagcagcttg agtagcagtc gttctttctc
    54541 ggatacgcta ccaggcaatg acaaatgaac cgttgtaccg aactcttgca gaaaaacacg
    54601 aaaatcacat gaattcatga acacaccttt tatcttttat cgctgttcag tatgagtgat
    54661 tacctgtgga ttcctgagca cacataaggg gtaaccataa acatcagcga tgccatacgc
    54721 ataacatccc acatcactat caaaaagatt atctttacct ctgatgctcg ctcaaaaaca
    54781 gttacgctaa ataccttaaa aaggtaatca caagtcaaac tcaggagata atcacgcaag
    54841 aatgcaatat taaaattcag atactcattt ttggtaataa agaagtagtt ggactatttc
    54901 aatatttata atttcccact acgtattgac gcccattcat gagtaaaggc gcattatatg
    54961 aatttaacaa tccagatcaa ctgaaaatac ctcaacctca taaacacata gcgtcaacat
    55021 tcaatgacat aatgagtaaa gatgttggtt atgcatacac gtcattactc tatgcctatc
    55081 ccttaacaac ctgcttttta aggctgaatc cattcagcaa tagatagcca acattataac
    55141 aatgacaata aaaacaatat ttaaaacaat caataaaata tcagacacag acaattaaac
    55201 cttaaacaac aaacagttac attgatttta gttgatttgc gctacgcgtt tcccatgaat
    55261 aaagcgtcat ttcaaattag caccaatatt tacattaaat aagactaagc ttattgaaca
    55321 ttcacaacag tccaagcgta ggttttgggc ttcaatagtt cttttctttt ttattatctg
    55381 ttgcctggca atcatcaatc gatactaagg gcttaactgt acacagatat aacgtatcaa
    55441 gaggtaagag aaaccgcgcc atatgcagac atggcgcggt tttatgcaat aacaatcacg
    55501 cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatggata aagttaaggt
    55561 gccgctcgta ctgatcgaga atatcggtga tcacctgctc tttgctgtag tccatcaggt
    55621 cgttgccctg gctgccttct aacaggaaag tttccagccg gtagtaggtc gatttaccgc
    55681 tacgtgcgcg gtaggtaaag cccggcaccg aatattgctg cggccaaatc tgatagacaa
    55741 agttttgctc ttcgcccata tgcaccaaca aatccagatg ccccaactgt tgtccctctt
    55801 ccggcggcag gctttttagc tccacgtacg cgccgcgcaa ccgcagctcc tgcgccactt
    55861 cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc gtgcccggat
    55921 aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc cccagcggtc
    55981 gcggcgcggt atcacggttg gcactttcac ggcggtaatc ttctaccttc agagatttat
    56041 acaaccccgc catcacgaag aaaatcacaa agctgaacgg cagccccatg attaccgtgg
    56101 tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc aggccaatcg
    56161 ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct ttaagctgcg
    56221 aggtgaaatt ccccagcacc agcgccccgg agtccgccga ggtcacataa aacagcaggc
    56281 cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atactgcgcc agcaggctgt
    56341 agaagccgcg ctccggatgg accatcgctt cctcggcaaa tgccgcgccg ccgtggatga
    56401 tttcatacag cgcgctattg ccgaacaccg agagccataa cagcgtgaag gtaaacggaa
    56461 taatcaacgt gcccagcacg aactggcgaa tggtacgccc acgcgagata cgcgccagga
    56521 acaggccgac aaacggcgac catgccaccc accatgccca gaagaagagc gtccagttat
    56581 tcatccactc aaccggacgg tcgaaggcaa aactgttgag cgtcatgccc ataaagcgat
    56641 tcacatagtc gccaacgttc agcaccagcg cattaagcag gaacgaggtg tcgcccataa
    56701 acagcacgaa caggatcaac cccagcgcca gcgcaacgtt gagttccgat aacacgcgaa
    56761 tgcccttatc gacaccggag gtgacagaga tcgtggcgat gatcaccgac aaggcgatca
    56821 gtgccgcttt cgccgccatt gaatcgggaa tatcaaacag tacgctcaag ccatagttaa
    56881 gctgtaccac accgataccg agcgtagtgg caataccgaa gatggtgccg attaccgctg
    56941 caatatccac tgaatgacca atcggcccgt taatccgttt accgaagatc gggtacagcg
    57001 ccgagcggat ggtgagcggc aaattataac gatagctaaa gtatccgagc gccatgccca
    57061 tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag accatcgcct
    57121 gacgcgcagc ctcaatcgtc tgacccgcgc cttccggcgg ctgcatatac tgcgttaccg
    57181 gttcggctac ggagaagaac atcaggtcga taccgatccc ggcagcaaac agcatcgccg
    57241 cccaactcag cagactgaat tccggtttgg attgttctgg cccgagcttc accgaaccaa
    57301 aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc agcagatagt
    57361 accaaccgaa ggttttagag acccagtcca gcgtgcgtcc aatccacagg gccgagaagt
    57421 cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggaa gtgtaaaaca
    57481 ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc cagtatcccc
    57541 actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt ttatattgaa
    57601 cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt ggcgaaaatg
    57661 cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac gctggaagca
    57721 ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg tgcaggcgtt
    57781 tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga agcaaccatg
    57841 cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc gattacatgc acttccgcag
    57901 ggcagtgcag agcagcgatt acaggcgatt gttgacggga atttcgatga aacgcaggtc
    57961 agtaatgctg cgatgaaagc ctggttggcg ttttgggcca gcagtatgca tcagccaatg
    58021 ctttaccgtt tgcagcaggt gagcagccgc cgcttgttgt cgaatctggt gagcgaattt
    58081 caccgcgagt taccgcgcca acaggcacag gaagcgggct acggccttgc cgcgctgatt
    58141 gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc tctcgcacac
    58201 tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag gagacgtgat
    58261 gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg ccaccagcgg
    58321 tcgcaccttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg tgcaggccgc
    58381 cgggcgtgag gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa aaatctgggc
    58441 ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg gccgttgata ttctgcgtga
    58501 acgcaataac gaactcgcaa aactggaaac cctcgacacc ggaaaagcgt attcggaaac
    58561 ctcaaccgtc gatatcgtta ccggcgcgga cgtgctcgag tactacgccg ggctgatccc
    58621 gtcgctggaa ggcagccaga tcccattgcg tgagacgtca tttgtctata cccgccgcga
    58681 accgctgggc gtagtggcag ggattggcgc atggaactac ccgatccaga ttgccctgtg
    58741 gaaatccgcc ccggcgctgg cggcaggcaa tgcaatgatc ttcaaaccga gcgaagtcac
    58801 cccgcttacc gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc cggacggtgt
    58861 atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg atcatccggg
    58921 cattgccaaa gtgtcattta ccggcggtgt cgccagcggc aaaaaagtga tggctaactc
    58981 ggcagcttcc tccctgaaag aagtgaccat ggaactgggc ggtaaatcgc cgctgatcgt
    59041 tttcgacgat gcagatctcg atctcgccgc tgatatcgcc atgatggcga acttcttcag
    59101 ctccggtcag gtgtgtacca acggcacccg cgtcttcgtt ccggcgaaat gcaaagccgc
    59161 atttgaacaa aagattctgg cgcgcgttga gcgcattcgc gcgggcgacg ttttcgatcc
    59221 gcaaactaac tttggcccgc tggtcagctt cccgcatcgc gataacgtgc tgcgttacat
    59281 cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac tgaaaggcga
    59341 tggcttcgat aacggcgcat gggttgcacc gaccgtgttc accgattgcc gcgacgaaat
    59401 gaccatcgtg cgcgaagaga tcttcgggcc ggtgatgtcg cttctcactt atgaatctga
    59461 ggacgaagtg attcgccgcg ccaatgatac cgactacggc ctggcggcgg gcatcgtgac
    59521 ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta tttgctggat
    59581 caacacctgg ggcgaatccc ctgctgagat gcccgttggc ggctacaaac actccggcat
    59641 tggtcgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt ccatccaggt
    59701 tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa tttgactaca
    59761 tcattattgg tgccggctca gccggcaacg ttctcgctac ccgtctgact gaagatccga
    59821 acacaaccgt gctgctgctt gaagcaggcg gcccggacta tcgctttgac ttccgcaccc
    59881 agatgcccgc cgcgctggca ttcccgctac agggtaaacg ctataactgg gcctatgaga
    59941 cggaaccgga accgtttatg aacaaccgtc gcatggagtg cggacgcggt aaggggcttg
    60001 gcgggtcatc actgatcaac ggcatgtgtt acatccgtgg taacgcgctg gatctcgata
    60061 actgggcgca agaacccggt ctggagaact ggagttatct cgactgcctg ccctactacc
    60121 gcaaggccga gactcgcgat gtaggcgaga acgactacca cggtggcgat ggcccggtga
    60181 gcgtcactac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt gaagcgggcg
    60241 tgcaggcagg ctacccgcgc acggacgatc tcaacggcta tcagcaggaa ggttttggcc
    60301 cgatggatcg caccgtcacg ccgcatggcc gtcgcgccag caccgcgcgt ggctatctcg
    60361 atcaggccaa atcgcgccct aacctgacca ttcgtactca cgccatgacc gatcacatca
    60421 tttttgacgg caaacgcgcg gtgggcgtcg agtggctgga aggcgacagc accattccaa
    60481 cccgcgcgac agcgaataaa gaggtgctgt tatgtgcagg tgcgatcgcc tctccgcaga
    60541 tcctgcaacg ttccggcgtg ggtagcgctg aactgctggc ggagtttgat attccgctgg
    60601 tgcatgattt acccggcgtc ggcgaaaatc ttcaggatca cctggagatg tacctgcaat
    60661 atgagtgcaa agaaccggtt tccctctacc ctgccctgca gtggtggaat cagccgaaaa
    60721 tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac tttgaagcag
    60781 gcggatttat tcgcagccgt gaggaatttg cgtggccgaa tattcagtac catttcctgc
    60841 cagtggcgat taactataac ggctcgaacg cagtgaaaga gcacggcttc cagtgccacg
    60901 tcggatcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc gacccgcacc
    60961 agcatccagc gattctgttt aactacatgt cgcacgagca ggactggcag gagttccgcg
    61021 acgcaattcg catcacccgc gagatcatgc atcaaccggc gctggatcag tatcgtggcc
    61081 gcgaaatcag ccccggtgtc gaatgccaga cggatgagca gctcgatgag ttcgtgcgta
    61141 accacgccga aaccgccttc cacccgtgcg gtacctgcaa aatgggttat gacgagatgg
    61201 cagtggttga cggcgaaggc cgcgtacacg ggctggaagg cctgcgcgtg gtggatgcgt
    61261 caattatgcc gcagattatc accggcaatt tgaacgccac gactattatg attggcgaga
    61321 aaatggcgga tatgattcgc gggaaggatg cgttgccgag gagcacggcg ggatattatg
    61381 tggcaaatgg gaggccggtg agagcgaaaa aatgagtcgt gatgtgaact gactcaggaa
    61441 cctatcagat tgagagtta
//
LOCUS       DARNVM010000009        60939 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.009, whole genome shotgun sequence.
ACCESSION   DARNVM010000009 DARNVM010000000
VERSION     DARNVM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.009"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..335)
                     /locus_tag="SI621_000540"
     CDS             complement(<1..335)
                     /locus_tag="SI621_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEI4063294.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNTVKNWIKGYRK
                     SGNEAFIRR"
     gene            complement(440..2395)
                     /gene="topB"
                     /locus_tag="SI621_000541"
     CDS             complement(440..2395)
                     /gene="topB"
                     /locus_tag="SI621_000541"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEI4063295.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPGLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(2400..3443)
                     /gene="selD"
                     /locus_tag="SI621_000542"
     CDS             complement(2400..3443)
                     /gene="selD"
                     /locus_tag="SI621_000542"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEI4063296.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3560..4111)
                     /locus_tag="SI621_000543"
     CDS             complement(3560..4111)
                     /locus_tag="SI621_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HEI4063297.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSALLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4272..6128
                     /gene="sppA"
                     /locus_tag="SI621_000544"
     CDS             4272..6128
                     /gene="sppA"
                     /locus_tag="SI621_000544"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEI4063298.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAIGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADISITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6294..7310
                     /gene="ansA"
                     /locus_tag="SI621_000545"
     CDS             6294..7310
                     /gene="ansA"
                     /locus_tag="SI621_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEI4063299.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKVHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7321..7962
                     /gene="pncA"
                     /locus_tag="SI621_000546"
     CDS             7321..7962
                     /gene="pncA"
                     /locus_tag="SI621_000546"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEI4063300.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(8055..9413)
                     /locus_tag="SI621_000547"
     CDS             complement(8055..9413)
                     /locus_tag="SI621_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063301.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSVRLPPLTLNQSNSKANVINGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9531..10289)
                     /locus_tag="SI621_000548"
     CDS             complement(9531..10289)
                     /locus_tag="SI621_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4063302.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10426..11406)
                     /gene="ydjG"
                     /locus_tag="SI621_000549"
     CDS             complement(10426..11406)
                     /gene="ydjG"
                     /locus_tag="SI621_000549"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HEI4063303.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDQQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(11416..12363)
                     /locus_tag="SI621_000550"
     CDS             complement(11416..12363)
                     /locus_tag="SI621_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEI4063304.1"
                     /translation="MDNLDIICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRVGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSFVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12368..13204)
                     /locus_tag="SI621_000551"
     CDS             complement(12368..13204)
                     /locus_tag="SI621_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEI4063305.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDTDIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(13225..14268)
                     /locus_tag="SI621_000552"
     CDS             complement(13225..14268)
                     /locus_tag="SI621_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEI4063306.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14285..15664)
                     /locus_tag="SI621_000553"
     CDS             complement(14285..15664)
                     /locus_tag="SI621_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063307.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESHGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            complement(15691..16767)
                     /locus_tag="SI621_000554"
     CDS             complement(15691..16767)
                     /locus_tag="SI621_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEI4063308.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAINVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(17137..17409)
                     /locus_tag="SI621_000555"
     CDS             complement(17137..17409)
                     /locus_tag="SI621_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HEI4063309.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIVMFK"
     gene            complement(17451..17864)
                     /gene="msrB"
                     /locus_tag="SI621_000556"
     CDS             complement(17451..17864)
                     /gene="msrB"
                     /locus_tag="SI621_000556"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEI4063310.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQSKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18206..19201
                     /gene="gapA"
                     /locus_tag="SI621_000557"
     CDS             18206..19201
                     /gene="gapA"
                     /locus_tag="SI621_000557"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEI4063311.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19285..20169
                     /locus_tag="SI621_000558"
     CDS             19285..20169
                     /locus_tag="SI621_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEI4063312.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(20220..21074)
                     /gene="yeaE"
                     /locus_tag="SI621_000559"
     CDS             complement(20220..21074)
                     /gene="yeaE"
                     /locus_tag="SI621_000559"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HEI4063313.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEMVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     EKTALDMV"
     gene            complement(21164..21910)
                     /gene="mipA"
                     /locus_tag="SI621_000560"
     CDS             complement(21164..21910)
                     /gene="mipA"
                     /locus_tag="SI621_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HEI4063314.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22346..24280
                     /gene="yeaG"
                     /locus_tag="SI621_000561"
     CDS             22346..24280
                     /gene="yeaG"
                     /locus_tag="SI621_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HEI4063315.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24393..25676
                     /locus_tag="SI621_000562"
     CDS             24393..25676
                     /locus_tag="SI621_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEI4063316.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            25823..27298
                     /locus_tag="SI621_000563"
     CDS             25823..27298
                     /locus_tag="SI621_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEI4063317.1"
                     /translation="MIRSTRVSLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            27479..28969
                     /locus_tag="SI621_000564"
     CDS             27479..28969
                     /locus_tag="SI621_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEI4063318.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRTICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            29012..29515
                     /gene="yeaK"
                     /locus_tag="SI621_000565"
     CDS             29012..29515
                     /gene="yeaK"
                     /locus_tag="SI621_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HEI4063319.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(29516..29620)
                     /locus_tag="SI621_000566"
     CDS             complement(29516..29620)
                     /locus_tag="SI621_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063320.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            29790..30236
                     /locus_tag="SI621_000567"
     CDS             29790..30236
                     /locus_tag="SI621_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEI4063321.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(30193..31014)
                     /gene="nimR"
                     /locus_tag="SI621_000568"
     CDS             complement(30193..31014)
                     /gene="nimR"
                     /locus_tag="SI621_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HEI4063322.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTSTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            31111..32292
                     /gene="nimT"
                     /locus_tag="SI621_000569"
     CDS             31111..32292
                     /gene="nimT"
                     /locus_tag="SI621_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HEI4063323.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     FLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDTNGDWSIPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            32347..32694
                     /locus_tag="SI621_000570"
     CDS             32347..32694
                     /locus_tag="SI621_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEI4063324.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(32716..32970)
                     /gene="yoaF"
                     /locus_tag="SI621_000571"
     CDS             complement(32716..32970)
                     /gene="yoaF"
                     /locus_tag="SI621_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HEI4063325.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGIANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            33153..34178
                     /gene="dgcP"
                     /locus_tag="SI621_000572"
     CDS             33153..34178
                     /gene="dgcP"
                     /locus_tag="SI621_000572"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HEI4063326.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            34211..34309
                     /locus_tag="SI621_000573"
     CDS             34211..34309
                     /locus_tag="SI621_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HEI4063327.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            34312..34386
                     /gene="yoaJ"
                     /locus_tag="SI621_000574"
     CDS             34312..34386
                     /gene="yoaJ"
                     /locus_tag="SI621_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HEI4063328.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(34445..34693)
                     /locus_tag="SI621_000575"
     CDS             complement(34445..34693)
                     /locus_tag="SI621_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEI4063329.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(34841..35023)
                     /locus_tag="SI621_000576"
     CDS             complement(34841..35023)
                     /locus_tag="SI621_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HEI4063330.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(35027..35386)
                     /gene="yeaR"
                     /locus_tag="SI621_000577"
     CDS             complement(35027..35386)
                     /gene="yeaR"
                     /locus_tag="SI621_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HEI4063331.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(35559..36197)
                     /gene="leuE"
                     /locus_tag="SI621_000578"
     CDS             complement(35559..36197)
                     /gene="leuE"
                     /locus_tag="SI621_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HEI4063332.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(36324..37247)
                     /gene="dmlR"
                     /locus_tag="SI621_000579"
     CDS             complement(36324..37247)
                     /gene="dmlR"
                     /locus_tag="SI621_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HEI4063333.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            37350..38435
                     /gene="dmlA"
                     /locus_tag="SI621_000580"
     CDS             37350..38435
                     /gene="dmlA"
                     /locus_tag="SI621_000580"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HEI4063334.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            38686..40296
                     /gene="yeaV"
                     /locus_tag="SI621_000581"
     CDS             38686..40296
                     /gene="yeaV"
                     /locus_tag="SI621_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HEI4063335.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEAPEKTPVLPAGTVFKGDN"
     gene            40328..41452
                     /gene="yeaW"
                     /locus_tag="SI621_000582"
     CDS             40328..41452
                     /gene="yeaW"
                     /locus_tag="SI621_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HEI4063336.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEEQLPGLGAKVLEACPEVHDLKLAARFTTLTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            41507..42472
                     /gene="yeaX"
                     /locus_tag="SI621_000583"
     CDS             41507..42472
                     /gene="yeaX"
                     /locus_tag="SI621_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HEI4063337.1"
                     /translation="MSDYQMFEVQVSQIEPLTEQVKRFTLVATDGKSLPAFTGGSHVI
                     VQMSDGDNEYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAAHLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(42519..43634)
                     /gene="rnd"
                     /locus_tag="SI621_000584"
     CDS             complement(42519..43634)
                     /gene="rnd"
                     /locus_tag="SI621_000584"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEI4063338.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(43716..45401)
                     /gene="fadD"
                     /locus_tag="SI621_000585"
     CDS             complement(43716..45401)
                     /gene="fadD"
                     /locus_tag="SI621_000585"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HEI4063339.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRNALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(45606..46187)
                     /gene="yeaY"
                     /locus_tag="SI621_000586"
     CDS             complement(45606..46187)
                     /gene="yeaY"
                     /locus_tag="SI621_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HEI4063340.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(46227..46922)
                     /gene="tsaB"
                     /locus_tag="SI621_000587"
     CDS             complement(46227..46922)
                     /gene="tsaB"
                     /locus_tag="SI621_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEI4063341.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAGDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(46980..48890)
                     /locus_tag="SI621_000588"
     CDS             complement(46980..48890)
                     /locus_tag="SI621_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEI4063342.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            49022..49366
                     /locus_tag="SI621_000589"
     CDS             49022..49366
                     /locus_tag="SI621_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEI4063343.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            49728..50087
                     /locus_tag="SI621_000590"
     CDS             49728..50087
                     /locus_tag="SI621_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HEI4063344.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(50207..50386)
                     /locus_tag="SI621_000591"
     CDS             complement(50207..50386)
                     /locus_tag="SI621_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HEI4063345.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            50460..51821
                     /gene="pabB"
                     /locus_tag="SI621_000592"
     CDS             50460..51821
                     /gene="pabB"
                     /locus_tag="SI621_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HEI4063346.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NTRRAWLESQQFSPQEDFALTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HTTYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            51825..52403
                     /locus_tag="SI621_000593"
     CDS             51825..52403
                     /locus_tag="SI621_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEI4063347.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            52587..53951
                     /gene="sdaA"
                     /locus_tag="SI621_000594"
     CDS             52587..53951
                     /gene="sdaA"
                     /locus_tag="SI621_000594"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEI4063348.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVIYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            54082..55680
                     /locus_tag="SI621_000595"
     CDS             54082..55680
                     /locus_tag="SI621_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEI4063349.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(55684..57240)
                     /gene="yoaE"
                     /locus_tag="SI621_000596"
     CDS             complement(55684..57240)
                     /gene="yoaE"
                     /locus_tag="SI621_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HEI4063350.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            57703..58674
                     /gene="manX"
                     /locus_tag="SI621_000597"
     CDS             57703..58674
                     /gene="manX"
                     /locus_tag="SI621_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEI4063351.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            58737..59537
                     /gene="manY"
                     /locus_tag="SI621_000598"
     CDS             58737..59537
                     /gene="manY"
                     /locus_tag="SI621_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HEI4063352.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            59550..60401
                     /gene="manZ"
                     /locus_tag="SI621_000599"
     CDS             59550..60401
                     /gene="manZ"
                     /locus_tag="SI621_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HEI4063353.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            60457..60915
                     /locus_tag="SI621_000600"
     CDS             60457..60915
                     /locus_tag="SI621_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HEI4063354.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT        15267 a        15536 c        14623 g        15513 t
ORIGIN      
        1 cagcgcctga taaaggcctc attaccagat ttgcggtagc cttttatcca gtttttaacc
       61 gtgttgtggc tgggaatgtt aaaccgggca gctacccggg gtagagactc agaatgcccg
      121 agagcataaa gaaccacgca aagtttatct tcaggagaat agctacgctt gtgtctgcaa
      181 tccagtgccc gaggaccgtg aagaagaaaa agattaatcc agtgtgagag gctggtgtga
      241 gaaagttgaa agagttttgc cgtagaaatg atgcctgcat gaccagccag gtaatgattt
      301 acagcggcaa gttttacttc aaatgaatgt ttcataaact gcaccttcag atgtcagatt
      361 atgtccaaca tttggggtgc agttcaaaac gccttatccg gcaataaaaa tcttcagtat
      421 cagatggttg aaagaacctt tatgttatca ctccattgcc cgcctcttct gatggcgggc
      481 tttttttcgg cgctctcttg cgtggcgaat cctttttctt cccttctcca ccgccgggag
      541 ccattattcc ccgaaactgc cttaccgacg tgcgctttgc ctgatcaatc aactggtaca
      601 atgtccccac cagcggctgc ataaagtcct gatagcgaca ctgcttttca ctgatttgcg
      661 tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt cgccatctct ggcagcgaat
      721 ggaataatgc ttttccggcg tcggtggagt ggatatagcg cccttttttg gtcaggaagc
      781 ctcgcttgaa cagcagttca ataatcccgg cgcgagtcgc ttctgtcccc agaccatcgg
      841 tcgcacgaag gatctttttc agatctttgt cctgcacaaa acgtgctatc ccggtcatcg
      901 ctgaaagcag tgttgcatcg gtaaaatggc gcggcggctg ggtttgccgc tctaccactt
      961 cacctttttc acacagcaac tcatcgcctt tcgccaccac aggcagcggc gtgccgtcgt
     1021 tttcttcatc gcgctctttg ctgcctaaca gtgtgcgcca gcctgcttca gcaagaaaac
     1081 gggctttagc gacaaatttg cctttggcaa tgtccagttc gataacacac ttgcggaaca
     1141 ccgcatccgg gcagaactgc atcaagtact gacgggcaat caggttatag accttcgctt
     1201 cgttctccgt caggttgatc gcagaactcc gtgcggtcgg gatgatggcg tggtgcgcat
     1261 cgaccttttt gtcatcccag cagcggttgc gtatatctgg atcgaccact ggctgcggta
     1321 acaaccccgg tgcatgaacg ctgatggcat tcatcactgc gtggcgtccg gcaaaatgct
     1381 cttccggcag atagcgacaa tcggaacgcg gataagtgat tagcttgtgc gtttcgtaca
     1441 acttctggca gatatcaagc acgttctgcg cgctcagacc aaagcgtttt gccgcttcaa
     1501 tctgcaacgc cgaaagcgaa aacggcagcg gtgcggattc tgattcccgt ttatcgttat
     1561 agctggtgac aatagccggt tggccgctga tgcggttgac cacatgctcc gccagcggac
     1621 gatgtaacaa gcgcccttct tcatcctgat aaggttcaca cgcttcgctc ggttgccaga
     1681 tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc tttgacttca aagaaatctt
     1741 tcgccacgaa gttttcaatc tcttcgtcgc ggcgcaccac cagtccgagt acgggcgtct
     1801 gcacgcgtcc cacggaaagt acgccctgat aacccgcatt gcgaccgaga atggtgtaag
     1861 cgcgggtcat attgatgccg tacagccagt cggctcgggc tcgcgccagt gcagaaacgc
     1921 acaacggcac aaactcactg ttggaacgaa gacggtcgat cgcccgctca accgcctgcg
     1981 ggttcaggtc gtttatcaag caacgctgca cctgctggcg cttttccggt gccagttgca
     2041 gatagtccag cacttcatcc accagtaatt gcccttcgcg atccgggtca ccggcgtgaa
     2101 cgatttcgct ggcttcatgc aggaagcgtt tgatgacatt aagttgtttg gtcacggagg
     2161 gtcggggctg taattgccac ttttccggga caatcggcaa atccgcaaga ttccagcgcg
     2221 catagcggct gtcgtaggcg tctggctgcg cctgctcaag caggtgaccg atacaccagg
     2281 tcaccacctg accattaccg cactcgataa agccatcgcc tttccggtgc ggtttgggca
     2341 ggacatcagc aatggcacgc gccagactcg gtttttcggc aataaacaac cgcattgaat
     2401 taacggatct caaccatggc acgaccgccg cgcgctggca ccagttcgcc aatcgccgtc
     2461 agttcaatgc caaactcggc ggctgtagct ttaacctcat tttctgcttc cggcatgacc
     2521 gccagcagca gaccgccaga agtttgcgga tcgcacagca gatcgcgcac ttcacgcggc
     2581 atttcaccca tcagatgacc gtagctggca aagttacgtt cggtgccgcc agggactgcg
     2641 cccaacttaa tgtactcttc aacccccggc agtttcggga tcgcttcata gtcgacgcgc
     2701 gcctgcacac cagcaccctg acacatttcg cttaaatgcc ccagcagacc aaagcccgta
     2761 acgtcggtca tcgcttttac gccttcgatg ttggcaaagg acgcgcctgc gatgttcatc
     2821 cggcacatca cttccgtcgc cagtccctga tgttctggtt tcagcagtga ttttttctca
     2881 gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga acagtttaca tccggcttgt
     2941 gcggtactgt ttttcttcac tcgctcggtc ggtacgatcc ccgttaccgc cagaccaaaa
     3001 atcggctccg gcgcatcgat ggagtgaccg ccagccagcg caatacccgc ctgacgacat
     3061 gcatagcgtc caccttcggt cacttcgcgg gcaatttccg gggaaagttt attaatcggc
     3121 cagccgagga tcgcaatcgc cataatcggc ttgccaccca tcgcgaagat atcgctgatg
     3181 gcgttagtcg ccgcaatgcg gccgaaatcg aaaggattat caacgatcgg cataaagaag
     3241 tcggtggtac tgataacgct ggtgccattg cccagatcgt acaccgccgc atcgtcgcgg
     3301 gtttcattac ccacaagcaa attcggatca acaaacttcg cctgctcact gtgcaggatg
     3361 gtttccaaca cttttgggga aattttacag ccgcaaccag ctccgtggct gtattgggtc
     3421 aaacgaatcg agttctcgct catggacatc tcctgtcaat gcaatccggg tatggtaacc
     3481 ctcattccgt gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc tcacaatcca
     3541 gacagtttcg cgacaattat cagaaataag tgacaaacgg cgtcgggtcc gggacgttaa
     3601 tcgacgtaga tgctttcagc tgcggcgtac caaggtagag aaaaccgaca attttatcct
     3661 gctcacggca accgaatgct tcacgcacta ccggactttc agttaatgcg ccactgcgcc
     3721 agatgccgcc aaacccctgg gcaactgctg ccatttgcat cgccatgacc gcgcatcccg
     3781 cagacatttc ctgttcccag cgcgggactt tatgattttc ttcgcatttt gccaccaccg
     3841 ttatgatgag cggtgcgcgg aacggcgcat tacgcgcttt gtcgatagct ttgtcatcac
     3901 taccggcagc aatggccccc tgttccaata atgcgctgaa acgctcgcgc ccttcccctt
     3961 caatcacaaa aaaatgccac ggttgcatgg acttatggtc cggcgcacgc atacccgcac
     4021 gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc agccaggcgg gaggcgctac
     4081 ggcgattgat caatagttcg agtgcatcca tttgattaac tcctgtcgtg atatttattc
     4141 acaaaattaa cacgagagtg gattttgtta cagcacagtc cgcaattcct gctgacaagt
     4201 gccggttggg tcattacgat aaccacatct attgcgcctg tgacaggtgt gacctttagt
     4261 tgggagaata catgcgaacc ctttggcgat ttattgccgg attttttaaa tggacgtggc
     4321 gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt ctttattttt ctcgtgctgg
     4381 ttggtgtggg gatctggatg caggtcagtg gtggtgattc gaaagaaacg gccagtcgtg
     4441 gcgcgctgct gctggacata tctggtgtga tcgtcgataa acccgacagt tctcagcggt
     4501 ttagtaaatt aagccgccaa ctacttggtg ccagttccga tcgtctgcag gaaaactcac
     4561 tgtttgatat cgtcaacacc attcgccagg cgaaggacga ccgcaatatc accggtatcg
     4621 tgatggatct gaaaaacttc gcaggcggcg accagccgtc tatgcagtac atcggcaaag
     4681 ccctgaaaga gtttcgtgac agcgggaaac cagtttatgc cataggcgag aactacagcc
     4741 aggggcaata ttatctcgcc agtttcgcca ataaaatttg gctgtctccg caaggcgtgg
     4801 ttgatctgca cggctttgcc accaacggtc tgtactacaa atcgttactg gataagctga
     4861 aagtttccac ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa ccgtttattc
     4921 gtgatgatat gtcaccggca gcccgcgaag ctgacagccg ctggattggt gagctgtggc
     4981 aaaactatct gaatactgtt gccgctaacc ggcagatccc tgctcagcag gtattccctg
     5041 gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg cgataccgcg aaatatgcgc
     5101 tggaaaacaa gctggtcgat gcactggcat cgagtgcgga aatcgaaaaa actctgacca
     5161 aagagttcgg ctggagcaag actgataaaa attatcgcgc catcagttat tacgattacg
     5221 cattgaaaac gccggcagat accggtgaca gcatcggtgt cgtcttcgct aatggcgcaa
     5281 ttatggatgg cgaggaaact caggggaatg ttggcggtga taccactgcg gcacaaatcc
     5341 gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct gcgtgttaat agcccaggcg
     5401 gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg gcagcgggta
     5461 agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg tggttactgg atttccacac
     5521 cagctaatta cattgtggct aaccccagca ccctgaccgg ttctatcggt atcttcggcg
     5581 tgatcactac cgtagaaaat agtctggatt cgattggtgt tcataccgat ggcgtctcaa
     5641 cttcaccgct ggcagatatt tccatcacca aggcactgcc gccggaagcg cagcagatga
     5701 tgcagttaag cattgagaat ggctataaac gctttatcac gctggttgct gatgcgcgtc
     5761 attcgacgcc ggagcagatt gataaaatcg cccagggcca cgtctggacc ggtcaggatg
     5821 caaaagctaa cgggctggtc gatagtctcg gtgatttcga tgatgcggtc gccaaagcag
     5881 cagagctggc aaaagtgaaa cagtggcatc tggaatacta cgttgatgaa ccgaccttct
     5941 tcgacaaagt gatggacaac atgtctggtt ctgtccgggc aatgttgcca gatgcgttcc
     6001 aggccatgtt acctgcaccg ctggcctcgg tagcctctac cgttaaaagt gaaagcgaca
     6061 agctggcagc gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc tgcgccaacg
     6121 tgcgttaagt cttgtactga gtggccgaca gatcgtcggc cacatttttt ttacctcgac
     6181 gaatcctctt cccgctgtct cgccccatat ttccttatcc gcatagtatc aggtgcgctc
     6241 cccctgcctc acgtatatac ttttgctctt tcgatatcat tcatatcaat atcatgcaaa
     6301 agaaatcaat ttacgtcgcc tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg
     6361 gttatattcc ggtgtcaggt catctacagc gccaactggc gctgatgccg gaattccatc
     6421 gcccggagat gccagatttc accattcatg aatatacgcc gctgatggac tcttcagata
     6481 tgacgccaga agactggcag catattgctg aagatattaa agtgcattat gacgactatg
     6541 atggttttgt cattctgcac ggcaccgaca cgatggcgta taccgcctct gcgctgtcgt
     6601 tcatgctcga gaatctcggt aaaccggtta ttgtgacagg gtcacaaatc ccgctagctg
     6661 agttacgctc tgacggacaa attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc
     6721 ctatcaacga agtaactctc ttttttaata atcgacttta tcgtggcaac cgtaccacca
     6781 aagcccatgc cgatggtttt gatgcgtttg cctctccaaa ccttcctccg ttactggaag
     6841 caggtatcca tattcgtcgt ttgaatacgc cacccgcccc gcacggtgaa ggggaattga
     6901 tcgttcatcc aatcacccca caaccgattg gcgtagtgac gatttatcca gggatttctg
     6961 ctgacgtcgt gcgcaatttt ctgcgccaac cggtgaaagc attgattctg cgctcatatg
     7021 gcgtgggtaa tgcgccacaa aacaaagcct tcctgcagga attacaagaa gccagcgatc
     7081 gcggtattgt ggtggtcaac ctgacacaat gtatgtccgg taaagtgaac atgggtggtt
     7141 atgccaccgg taacgccctc gcccatgccg gcgttattgg cggtgcagat atgactgtag
     7201 aagccacact aaccaaactg cattacctgc ttagccagga actggatact gaaaccattc
     7261 gcaaggccat gagccaaaac ctgcgcggcg aactgacgcc ggatgattaa ggagactgca
     7321 atgccccctc gcgccctgtt actggtcgat ttacaaaatg atttctgtgc tggtggcgcg
     7381 ctcgccgtgc cggaaggtga cagtacggtg gatgtcgcta accgcctgat tgactggtgc
     7441 cagtcgcgcg gtgaagcggt tatcgccagt caggactggc acccggcgaa tcacggcagt
     7501 tttgccagtc agcacggtgt agagccttat acgccaggcc aactcgacgg tttgccacaa
     7561 accttctggc cagatcactg tgtgcagaac agtgaaggcg cacaattaca tccgttactg
     7621 aaccaaaaag cgatcgcagc ggtgttccat aaaggcgaaa atcctttagt tgacagttac
     7681 agtgcctttt ttgataacgg ccgtcggcag aaaaccacgc tcgatgactg gttacgcgat
     7741 catgaaatcg atgaattgat cattatgggc ctggctactg actattgcgt gaagtttacc
     7801 gtactggacg cgttacagtt aggttataag gttaatgtga ttaccgatgg ttgtcgtggt
     7861 gtgaatatcc agccacagga cagtgcgcac gcgtttatgg agatgtcagc agctggggca
     7921 acgctatata cgctggcaga ctgggaagag acgcaggagt aattttatgc tggcctacaa
     7981 ttctgtactg ccattgtagg ccagataaaa cacgtcagtg gtacatctgg caattgacgc
     8041 catcaacgaa agatttaatt cacctcagaa atctcttcca atgacacctt ccgcgtttcg
     8101 atgccaaaaa tggagagaac cagcgcacag agtactaaca tcacgccaag cactataaac
     8161 accgtgattg acccataatg cgttaatagt gccgcaacgc cataaggcgt gaagaccgcg
     8221 acgatccgtc cgacggcatt aacgaaacct gaaccacgca ggcgtaaatg cgttggccaa
     8281 agctccggga tataaaccgc cgacgcgaag caaacataca tgtataaaaa gaagatcatc
     8341 accaggccat agattaaaat cgcccactct gtagtctgaa ttgaatagat atagcctaac
     8401 acggcaataa taataagtaa ggcggagcca aataatcgac gaggaaaatg atcaataatt
     8461 aatgccgcaa taaatattcc taccggagcg ccaatcataa taacagcagt cattaatatt
     8521 gatttatcaa catcaatgcc ggagttaaca aatatggtcg gtatccatac ggtgatggta
     8581 taaagtgaaa tgttcattgc aattaaaaca gtaatcgcga ctaatgtacg tcgtaacatt
     8641 tcccctttaa acagcagcca gaaagtacca ttgattacgt tagccttgct gttgctctga
     8701 ttcaaagtaa gcgggggtaa acgaacactc ttctctcttt caatttgctg ctctacttca
     8761 cgaagttggc tttctgcacc tgcgatttgt cctttccccg ccaaccatcg aggcgactca
     8821 ataaagtatt tacctgagag aaaccaggct aacagtatgc caataccacc cagcagaaac
     8881 attatccgcc aactaaaaaa agcgataacc accacgccta tcgccgcaga caacatgggc
     8941 gaccagttac cgacaaatga gagccgcgcg gaccattttc cacgcaccgt cgcggggata
     9001 aactccgtaa acgaagcata gccaaccata atcagcgccc ccattcctgt gcccatcagg
     9061 aagcgaaaga agatgagcca gtacatatca gggacaaaag cggcccctgt tgccgcaata
     9121 ccgacgatga gaagatttat gcgaaaagcc ctgcgccgcc caaaataatc accgatgaac
     9181 ccaccggtaa gagagccgat gaaataacca aacattaatg ccgaggtaaa ggcggcattg
     9241 aggaaattat ttgaccatcc attgcttacc agctttgcta aaacaacatt gcctgaataa
     9301 ctcaaaaaac ccgttaataa cagactaaag cttataatac caaatatacg ataatgaaac
     9361 cgggccaaag gcaagcggtc cagtcttgcg ccaatttgat catattgttc cattaggtgc
     9421 ctcggatttg ctaaaaaggt agcaattgcg gcaaattcac ttgccgcatc tttatcatca
     9481 ttttattttc caggcgcagg aacaaataat attgacaacg tgtgcttaaa ttaccacacc
     9541 agctggatat tattgtcttt gcaaaatgct atccactctg cacccggtga tttatcagtt
     9601 attatgtaat taatatgact aaagtcagct aactggacaa aagctttgcg atcaaactta
     9661 gagtgatcaa ccaataacgc aacctctgtc gcctgacgga tcattgtctt tttgatttca
     9721 gcttccgctt cgttagagtc cagcgcgccg ctgttaatat ctagaccttt acaactcatt
     9781 accatgatat cgacatggta gcggctgatg atctctttag taattcttcc ttgcagggaa
     9841 agtgtatttt tgtttagttc cccacccgtt gaaacgactt taatttctga ctgagccaga
     9901 acatgaatcg cttccgccga gttggttagc aacgtcaggt cactacggtc ctgtaacaat
     9961 ttgagcaatt ccattacggt actactggaa tcggctgcca tggtggtttt attgtcgata
    10021 aagggtagtg ccttgcgtgc aataagctgc ttctcttcat aaaacgatga agcgcgctta
    10081 taaaaatgga tattctccgt caacatcgct gtatttaaaa cagcaccacc ataggttctg
    10141 gtcaaaaagc cttcatcttc cagcttctca agatcgcggc gaatggtttc ttcggttacc
    10201 tgaaaaatcc cactcaaatt tgagactgtc acctttttat cgttggcaac catttgctta
    10261 attgcctgaa tcctgtcttt tgccgccacg attacacccc tgtatctttt tacatcacat
    10321 tagcacgatt atcgcataat tgatgtttac tttcaaaata acctgtttga atcacagatt
    10381 ttcatcacag ttttcacaga aacagaggtg aatcgtgttg agtatttaac gctccagggc
    10441 ctctgccatt tccctcatca atgttgcgtc tgcatccgat aagttgatat tcaatgccgc
    10501 gacattttcg cgtacctgtt ccggtgcagt agccccacta agaatggaaa ttaaatcact
    10561 ctgttttaat atccacgcca gtgccagagt gggaattgtg cactgataac gagcacagag
    10621 tggctgccac tgttcaagca tatcaatcac tttcagcatg ttttcacgct ggaaccagac
    10681 tttatttgcc cgagcgccgc ccgggacgta atcacgagtg atggtaccgg tcaacaatcc
    10741 ctgctctaac ggggaataaa cctgtaccac aatgccatta tcacgacata gcggcagcag
    10801 ttcgctttcc attgcccggt cgaggatact gtatttcgcc tgaataatat ccagttcacc
    10861 atattgcaga tactcgcgga tatggtcagc atcgacgtta gcagcgccta tagcgcgaat
    10921 tttcccttcg gcctttaact cattgagcac tgcgacagtt tcagcgatcg gcgtaaaaaa
    10981 tggcggcacc gactgccagt gcgtcatgta gatatcgatg taatcaatac ccagacgttg
    11041 caagctggct tctacctctt cgcggataga ttccggggaa aggtttttat acaactgccg
    11101 atcgccaact ttgttgaata aacttccttt tcgttcccag acaatgccgc atttggtttc
    11161 tactacaacc tgttcacggg gcagtttttt taacgcctga ccgacgataa cttcactatt
    11221 gccaaagtta tatcctggcg cagtatcaat cagattaatg ccacaacgat gggcttcaag
    11281 aatcgtatca atacatattt gctgatcgag atcgccattc catgcaggac cgccgccaat
    11341 ggcccatgtc cccaacccca ttcgcgaaag cgtaatatcc gttgtgccta aaggtatctt
    11401 tttcatttgc cttcattatc cttcgtattc ttccagcaat tgttcgacca gctttctgtt
    11461 ttttacgccg gtggtggcac cgacgcttag aaccgagata gccgccgttg cattggcaaa
    11521 gcgtgcgcat tcacgcagat ttttgccttc taacagagcc gctataaagc cagaagcaaa
    11581 gttatcgccc gcgccaatag tgtcgatggc ggttattcct gcgaccgccg gcaccttcat
    11641 cgtcatgtca ccacgcttga taaagcagcc gtctttaccc gttttaatca ccaccgtttt
    11701 tacgccgcac gcaagaaagc agtcagcaat ttcatccagt gtctctttcc cggtgagtaa
    11761 ttttgcctcg gcaaaattag gaaacagata atcgacaaaa ctcaatgctt cgcaaatatc
    11821 atccagcgtt tcattcaacc gtggtttgat catatcggca cagataatca tctgccgggc
    11881 tttagcttgc gtaaaaattt ctgttagcgc tttaccatcc aatagaggac tgttgaaaat
    11941 actggccagt gataataatt tcgcctgaga aaaccgcgca aaatcaacat cgtcaatatt
    12001 caatttccac agactaccgt tacggtttgt gacaaacgtc cgctcgccat cctccgtcac
    12061 cagtccaacg ttaatagagg tatctatgct gacgtcctgc ttcaggcttt gaatatcaat
    12121 attctctttg cggcaatggt cgagaataaa ttgtcctgcg gcatctttac caacacgact
    12181 cattaatgct gtgcgatggc ccagacgaga aataattgtt gcttcattta ttgcatcacc
    12241 cccggtggtc attgcgattc tttcaagagg ataagaatcc acatcaaaga tatttttact
    12301 gaccggttgc aatggaatat caacaatagc ggcacctata caaataatgt cgagattatc
    12361 catatcatta ttccgctttg ccatcagaac cgaacagttt aattttttcc aatgcccgtt
    12421 cttttacagc tttacgtact tcacgttcta aatgcaggaa aggctgatcc tggttttctt
    12481 tcactgctac cattgcggcc tgacagagct ccgtatgaat gttgattttc gcgataccca
    12541 acgaaattgc agttttaatg tcggtatcac tgatccccga tgcgccgtgc aaaaccagcg
    12601 gaacagaaac ggcatcgcgt acgcgtttga cgacctcaaa gttcaattgt ggctctgacg
    12661 tataaacccc atgctggttg ccgatggcga cagccaatga gtcacagccc gtacgttcaa
    12721 caaattcagc cgcctggtca ggatcggtat agtgatagcc cgccagcgct tcttcataaa
    12781 cggtttcatt accgacatgc cctaattctg cctctaccgg aatacccagc ggatggaaga
    12841 aatcaacagc ctctttggtt aaacgaatat tttcttcgaa atcaaatgcg gaagcatcac
    12901 gcattaatga attcatacca tgagtccagg cgttatgaat aatttccata ctccgaccat
    12961 gatcccaatg agttattacc ggcaccgttg ctttttttgc cattgatacc atcatgtgag
    13021 agaaatcttc aaatgaggtg ttcccgacaa aacctgtacc aaaagaaata ataaccggtg
    13081 atttcgcttc ttcggctgcg tcgataacgc ccatcaacat ttctgcattc catacgttaa
    13141 aatgggcaat tgcataatgt ttatttgtag catcgttttc ccaatatctg atatctgcga
    13201 gcatattctg ttctcccgta ttaattaatc gctaatttta ataacgcctt taataatgtc
    13261 gcgtttgttg ttaaccgact cttcaaatgc ctgttgtaca tcctgataat cgtaaatatg
    13321 tgtcaccatc gatttcacat cgaatcgccc tgaggaaata gcttcaatcg tgaccggata
    13381 acgattggca tagcggaata ctgtctgaat agtgacttcg cgattgattt tgaggaaatt
    13441 gattgccgaa tcgcccggta cagtaccaac aatcataatt ttaccgccgc gcattaccag
    13501 ataaggtgcc tgtttaacgg tgaccgcaga acccgctgtt tcgaaaacaa tatctgcgcc
    13561 catttcttcg gtaaattgct gacagcgtgc aatagtgtct tcttttgcgc cgttaataac
    13621 cactgtcgcg ccgagctgtt ccgccattgt cagacgtttt tccagtacat caacgacggc
    13681 aatatccgta gctcccaggc atttgcacgc ttgcaacgtc atcaaaccaa tacaacccgc
    13741 tcccagaata attatcttct tacccggttt aacatctgcc agcatcgcgg catgcatccc
    13801 gactgcggca ggctccacca gcgccccttc catcgtgtcc atattgtcgg gcagtttgta
    13861 agtaaagctc tccggatgac acagatagtg cgttaatgcg ccgcggtagt taggttgtgt
    13921 cgccataaaa tcaacgtccg ggcagatgtt atatttgcct tccagacagt aacgacagtg
    13981 accgcaagga acgccaggtt cgatatttac ccgatccccc ggtttaaatt tgcgcacgcg
    14041 gcttcccaca gccacaaccg tcccggcgca ttcatgaccc aggccaattt cttgatttgg
    14101 gtctttaggc ggaataaacg ggcctgattc gaaaccatgt acatctgaac cacaaatacc
    14161 gacatattcc actttaatca aaacttcatc ttctttaggc acaggtattt ctgctgaaat
    14221 aattttcatt gtgcccggca cctgcaatat tgcttttgaa tttttcattt tgaatccacc
    14281 agtattattt attgactact gcatcaatac ttaaactttc aacggagaca cctttggttt
    14341 caattccaat ggtggcgata gcaattgcga caataattga aaccgccccc agaagaataa
    14401 agactcccgt tacgccataa ctactgagca gcactgcaac ggcataaggt gcggcaatac
    14461 cactgattcg ccctaccgca ttcgccagac cggagccacg taatttggcc tctgtcggcc
    14521 agatttcagg gacatacact gccgaggcat agcaaacgta catatagacg aaagtaatca
    14581 ggaagaaacc aatcagcgtt atgagcaaca tactggtttg cagtgaatag atatatccga
    14641 gcaccgcaat caggattaat agccccacac ccattgtttt acgcggaatt ttatccatca
    14701 ccagcatggc aataaaaata ccaaatggcg cgccaaacat actcatggta tttaaaacaa
    14761 tcgagtcttt taaattaatc ccctgggtca tgaatatcgt tggcaaccag ttaattaatg
    14821 tgtactgcac aacgttcatg gcaatcagca cacaagaacc taatatcacg cgtttcagta
    14881 atactcctgt cagtaaggct gaatacggca ccgcttgtgg cgctttaccg tcatcagcaa
    14941 taaccacggg cggcaaaggt ttacccgtct ggcgtatgac gccttcttct atactccgca
    15001 tgactttctc ggcttcctga taccgtccgt gcgattccag ccagcgcggg gattcaggaa
    15061 agtagcgcca ggccagcgcc gtagcgataa gcgacaatat tgcagggata agcagttgta
    15121 ctcgccagtt ccactctgca ctaatcagcg gcgtgagtcc catcgcaatc aatgaacaga
    15181 gcggatatga ccagttgcca ataaaggaaa cccgactgga ccacgttccg cgatttctac
    15241 cgggcatata ttcggtgaaa ccagcaaaca gcgtcaccag taaagctccc agcccaacgc
    15301 ccatcacaaa acggcaggca ataaggaaat ccatattcgg tgaaaaagca ccgaccacca
    15361 tcgaggcaat atgaatggcc tcatagagga tgaacgcatt tctgcgaccg gttttgtcac
    15421 cgatgatgcc gccaaccagc gcaccgagaa acattccggc ggtcgtgatt gctgagaatg
    15481 tggcagtggt ggaattatct gtccagccca acgctttcag ctgcgcgagg atcaagccac
    15541 caacggcatt actccagcag acaagcaagc caaacgcgac gatggcaaac attgacgaat
    15601 gccagcggca atccggtaag cgatccagcc gtgcaccaca atgcggtttt gttatctgtt
    15661 ccatgcgata acgttccttc aggagagtag ttattcatca aagtcgtaag tcatgatcac
    15721 tttgattgcg gttttatcga ccatcgcatc aaacccttcg cgccattgcg acaggccgat
    15781 acgatgcgtg atcatcggtt tgacgttgat agcgccgctg gctaatagcc ggagagcgtt
    15841 acgccatgag gtggagtcat aggccatatg cccaatgatg cttttgttcc aggcggtaat
    15901 gtcattaatc gagaaatcaa gaggtttgaa gcccattcca acgcgtacca cttccccgtt
    15961 cgggcggagc atttcgatgg cttgtttcaa tgcgatattg gcaccggagc attcaatcac
    16021 cagccccaga ttgtctttgc cacatatttg ctggcagcgc gccaccacat cttcggtaga
    16081 accatttact actgccgtcg cacccagttc ttttgcaacc ggaaagcgga ccgccacatc
    16141 ttcttgcaga ccaacgacga cgatatttac cgcccccata atccgcgcca tttgtacgga
    16201 gaacagcccg agtgggccag tgccgatgac gacaacatcc tgaccaggaa ggaatttcga
    16261 ttgctgcgcg atggatttat aggcattaca gatagggtca agtacggctg cgtcctcata
    16321 atcaacacca tcagggattt cccacaatgc atgacgatga attttgagaa tttcaccagg
    16381 aaccagacaa tatttggaaa aaccaccgcc ccaggtattg ttatccagac caaggtttac
    16441 tttttctgta caacacagaa aatcaccttg ttcacaggcc ggacaaacgc cgcagacgtg
    16501 accgctgtta tccgacacga cgcgttgccc cactttccag tctttgactt tttcaccaac
    16561 ctgcgcaata caacctgcga actcatggcc gcggatagag ttaaactcat cagaaccgct
    16621 atcgacattg tagtgcttca tgtctgcgcc gcagattgcc gcggctttaa tttcaatcac
    16681 tacatcttcc gggccacaaa tgggttgtgg cacatcgatc atcttgtagc cgccaaaggc
    16741 tttgccaaac cgagccagtg ctttcattgc gtcatcctct gtgttttgtt ttgcttagat
    16801 ggtcatcata cttacccaca tgatttttga ggtcaacatt aaatctgtaa aacacagata
    16861 atgatctgcg ttttacaact cagatcacaa ttacgcagaa aatggctggg tctgttgcag
    16921 ggtgatggaa ggtggaacgc aaaatgagca aatttgagag ggattaccgg ccaaataggt
    16981 ctgagactta agttcaggag aagtcattca ggcgtgaacg cgactgcaag agaccgggct
    17041 gggacttagc caggtcgggg cgagataatt gagatgagtc gtggggttat tgttggctat
    17101 cccctttata accgcggcaa aaaggggaca aaccacttac ttaaacatga caataggctt
    17161 tgctgagata ccaaaatctt cttttaactg ttgtttgctc ttcataacca tctgaccatt
    17221 tgtgtcaatt gtcatatgct gagcttcggt attatggcgg gcttgccaca gcatcaccaa
    17281 ttgcaggcag ttctcttttt gttcctcggt cagcgcaacg ccatctggcc acttacccag
    17341 ttcaacggct gtcgacaaac gctggtatac ctcaggcgtc atgctgttga taatgtcatc
    17401 aagattcata atttttccgc tcctgtggaa taagttgctg aatcgttttt tcaaccgttg
    17461 atttcttcgc cgttttcacc atcggtaaag cgtaaagagg ctgagttaac acaataacgt
    17521 tcacccgttg gctgcggccc gtcggggaag acatgcccca gatgggcatc acagttaccg
    17581 caacgaattt ctatgcgctg cattccatgt gacaagtctt tgatataacg aatggattct
    17641 tcactcaccg gttcgtagaa actgggccag ccacagccgg aatcatactt ggattgggaa
    17701 tgaaacagcg gggcatcgca gatcaaacag tgatatacgc cgtcacgctt gttatgcagt
    17761 aaacgacccg taaatggcgg ctctgtccca tgattctgcg tcacgtagaa ctgcatctcg
    17821 gacaaatttt ttttcagttc ttctgccgaa ggtttattag ccatttgctc acatctcact
    17881 ttaatcgtgc tcacattacg tgactgattc taacaaaaca ttaacaccaa ctggcaaaaa
    17941 tttgtcctaa acttgatctc gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt
    18001 tttatcgtaa ttgcccttta aaattcggga cgccgacccc atgtggtctc aagcccaaag
    18061 gaagagtgag gcgagtcagt cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat
    18121 tgacacgatt ccgcttgacg ctgcgtaagg tttttgtaat tttacaggca accttttatt
    18181 cactaacaaa tagctggtgg aatatatgac tatcaaagta ggtatcaacg gttttggccg
    18241 tatcggtcgc attgttttcc gtgctgctca gaaacgttct gacatcgaga tcgttgcaat
    18301 caacgacctg ttagacgctg attacatggc atacatgctg aaatatgact ccactcacgg
    18361 ccgtttcgac ggtaccgttg aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat
    18421 ccgtgttacc gctgaacgtg atccggctaa cctgaaatgg gacgaagttg gtgttgacgt
    18481 tgtcgctgaa gcaactggtc tgttcctgac tgacgaaact gctcgtaaac acatcaccgc
    18541 tggcgcgaaa aaagtggtta tgactggtcc gtctaaagac aacactccga tgttcgttaa
    18601 aggcgctaac ttcgacaaat atgctggcca ggacatcgtt tccaacgctt cctgcaccac
    18661 caactgcctg gctccgctgg ctaaagttat caacgataac ttcggcatca tcgaaggtct
    18721 gatgaccacc gttcacgcta ctaccgctac tcagaaaacc gttgatggcc cgtctcacaa
    18781 agactggcgc ggcggccgcg gtgcttccca gaacatcatc ccgtcctcta ccggtgctgc
    18841 taaagctgta ggtaaagtac tgccagaact gaatggcaaa ctgactggta tggcgttccg
    18901 cgttccgacc ccgaacgtat ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac
    18961 ttacgagcag atcaaagctg ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct
    19021 gggctacacc gaagatgacg tagtatctac cgatttcaac ggcgaagttt gcacttccgt
    19081 attcgatgct aaagcgggta tcgctctgaa cgacaacttc gtgaaactgg tatcctggta
    19141 cgacaacgaa accggttact ccaacaaagt tctggacctg atcgctcaca tctccaaata
    19201 agttgagatg acactgtgat ctaaaaagag cgacttcggt cgctcttttt tttacctgat
    19261 aaaatgaagt taaaggactg cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa
    19321 caaatctccc ctgtcctctc ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat
    19381 catccccagg taaaagcctc ttttgcatta cagggcgcac accttctctc gtggaaacct
    19441 gcgggtgaag aagaagttct gtggttgagc aataacacac cgttcaaaaa tggcgtcgct
    19501 attcgcggtg gcgtaccggt ttgctggccg tggtttggtc cggctgcaca acaaggtctg
    19561 cctgcgcacg gttttgcccg caacctgccg tggacgctga aatcacatcg tgaagatgct
    19621 gatggcgtag cgctgacttt tgaattgaca caaagcgaag agacgaaaaa attctggccg
    19681 cacgacttta cgctgttagc gcatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa
    19741 gcacacggcg aatttgaaac cacctctgcc ctgcatacct actttaacgt gggtgatatc
    19801 actaaggtaa gcgtcagtgg actgggcgat cgcttcattg ataaagtgaa tgacgcgaaa
    19861 gaagatgtac tgaccgacgg tattcagacc ttccctgacc gtaccgatcg cgtgtatctg
    19921 aatccgcaag attgcagcgt gattaatgat gaagcgctga atcgtattat cgccgtaggc
    19981 caccagcatc atctgaacgt tgtaggctgg aatccgggac cggcgctttc agttagcatg
    20041 ggcgacatgc cggacgatgg ctacaagacc tttgtttgtg tagaaacggc ttacgcttca
    20101 gaaacgcaaa aagtgaccaa agaaaaacct gcacatctgg cgcaatccat tcgcgttgcg
    20161 aaacgttaat ttgcgttaat gttgtgtgcc gggtgcgatg catccggcac gtatcaacat
    20221 cacaccatat ccagcgcagt tttttctttt ggtgccggat atgccttatc cagcatagct
    20281 aattccgctg atgaaagttc gacctcaagc acagccgcat tttgttggac atgggcaatc
    20341 gtggccgctt ttggaatcgc catcacaccc tgatgactga tcacccacgc caacaatact
    20401 tgtgccgcgc tgatattgtg agcatgtgca atttcgttga ctaccgcgtt ttttaacagc
    20461 ccattgcgca accgcccggc ctgggctaac ggactgtaag ccatcaccgg catctgctgt
    20521 ttctggcacc agggaagtag atcgtactca atccctcgtg aaccgagatg gtaaagcacc
    20581 tgattagtgg cacactgatt cccccccggc agctgccaga gttcctgcat atcggcataa
    20641 tcaaggttag aaacgcccca gcggcggatt ttgccctggg cgatcaattt ttccatcgct
    20701 gcgacagtct cttcgagagc gaaactgcca gaccagtgta ataagtagag atcgagataa
    20761 tcagtattga gacggcgtaa actggcttcg catgcattta tcgctttttg cccgccagca
    20821 ttccacggat agactttaga gacgagaaag accttctctc gcagaccggt taatgcttcc
    20881 ccaaccacca tttcggcacc gccctcggca tacatttcgg cggtatcaat gagggttaaa
    20941 ccgagctcaa tgcccgcgcg tagtgcagct acttctgttt tacgctgact ggcatcttcg
    21001 cccatatacc atgttccctg ccctatggct ggcagtgaga catcgccact aaattgaatc
    21061 attttttgtt gcattgtttc ctcccaggta ttgcaccacc gtaatgcaaa acagggcatt
    21121 acgccctgtt tttatgcaca aaatgccctg gaacgatgca ttatcagaat ttgtaggtga
    21181 tcccggtaga aatcaggcca gtccaggatt tatccaccat tgggctgtca gtaacttcat
    21241 cagacagacg ggtgtagcgc gcggtaccgt aaacactcca gtcgccgagg aagttgtagc
    21301 tggcgctcag ctccaggtaa gggctccagc tgtcgttcgg gttatagcca cgcagaccgc
    21361 tgcgagcgga ctctttgcgc gatacgccat aatagtattc gttctggttt tcgctgttcc
    21421 actgcacacc gatacccggg gtcacggtca ggccgccgtt ggtgtaacga tacaaccagg
    21481 ccatatccca gacaatgccg ttgctgttat ccagggtatc gccagccagg gtggtacgca
    21541 ggtaaccgta ctgggtaaag tgagcataag acagaccagc catcatggtg ctcttacggt
    21601 catccaggtg acgcatttgg tgatcgccac tgtctttcgc tttgaagtaa agcggcgacc
    21661 aataagcggt aattgaaagt ttgtccgttg cgtcattcca caggtagtaa ccaccaccta
    21721 agccacggaa ccagaagtta tcgccttcat agttgattac cggtactggg taaacatcgg
    21781 tatcgtaatc tttatatggg tgttcaacga cacctacgcc tgcgcccagg gaaaatttac
    21841 cttcagcgtg cgctacgcct gcagacgttg cgataagcac tccaagtgcc agaagtttga
    21901 gtttggtcac aattaatcat tccttaaaca aatgtttagc gggcgacaaa gtttacccgt
    21961 caatacatcg aaacccaacc tttttacgtt ttcatttttt aaagtaactg tttaattttc
    22021 ctgacgcgga tgacaccgcg cttacagcca aatgaattta gcgttactgg cgaccctggt
    22081 ctttacatta attatgcaaa atttatggat gagttgttga tatgccattg aaattaagaa
    22141 agccgtgcag gcaagttttc catttgccat ctacgcttaa ttttgaaggt gtatcaccgg
    22201 gcacgttgtt ctcatcgtcg ataaaatggc atgagagttg ctgtgtttta gcaagagacg
    22261 tcgttcagtt tacctcttcc gggagcctct actattcata tgaacggctc ttaacctgtg
    22321 ctaaaaaacg aaaggacggc ataccatgaa tatattcgat cactatcgcc agcgatatga
    22381 agctgccaag gacgaagagt tcacactgca ggagtttctt accacttgtc ggcaagatcg
    22441 cagtgcttat gccaacgcgg ctgagcggct attgatggct atcggtgagc ctgtcatggt
    22501 cgatacagcc caggaaccca gactttctcg actcttttct aaccgggtca ttgcacgtta
    22561 tccggcgttt gaagagtttt acggcatgga agacgcgatt gaacagattg tctcttatct
    22621 gaaacacgcg gctcaggggc tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt
    22681 gggtgggggt aaatcatcgc ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat
    22741 ttatgtattg agcgccaacg gtgagcgtag cccggtcaac gatcatccgt tctgtctttt
    22801 caatccgcag gaagatgcgc agattctgga aaaagagtat ggcattcctc gccgttatct
    22861 cggcaccatc atgtcgccgt gggcggcaaa acgcctacat gaatttggtg gcgatatcac
    22921 taagttccgg gtagtgaagg tctggccgtc aattttgcaa caaattgcta tcgccaaaac
    22981 ggaacccggt gatgagaaca accaggacat ctccgcgctg gttgggaaag tcgatattcg
    23041 taaactcgaa cactacgcgc agaatgaccc ggacgcctac ggctattccg gtgcactgtg
    23101 ccgcgccaac caggggatca tggaattcgt tgagatgttt aaagcaccga ttaaagtgct
    23161 gcatcccttg ctaaccgcca ctcaggaagg taactacaac gggacggaag gtatctccgc
    23221 cctgccgttc aacgggatta ttctcgccca ctcgaacgaa tccgaatggg tcactttccg
    23281 taataacaaa aacaacgaag ccttcctcga tcgtgtttac atcgtgaagg tgccgtattg
    23341 cttgcgcatt tccgaagaga tcaaaatcta cgagaaattg cttaatcaca gtgaattgac
    23401 tcacgccccc tgcgcccctg gcacgctgga aacactgtca cgtttctcca ttctttcgcg
    23461 cctgaaagag ccagaaaact ccagcattta ttcaaagatg cgggtttatg atggtgaaag
    23521 tctgaaagac accgatccca aagccaagtc gtatcaggaa tatcgtgact acgccggtgt
    23581 cgatgaaggg atgaacggtc tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt
    23641 caacttcgat catgtagaag tggcggcaaa cccggtccat ctgttctacg tcctggaaca
    23701 gcagatcgag cgcgagcagt tcccgcaaga gcaggcagaa cgctatctgg agttcctgaa
    23761 aggttacttg atcccgaaat atgccgagtt tatcggcaaa gagatccaga cggcctacct
    23821 tgaatcctat tccgaatatg ggcaaaacat tttcgaccgt tatgttacct acgcggattt
    23881 ctggattcag gatcaggagt atcgcgatcc agataccggg cagctgtttg atcgcgagtc
    23941 tcttaacgcc gagctggaga aaatcgagaa accggcgggg atcagtaatc caaaagattt
    24001 ccgcaacgag attgttaact tcgtactgcg cgccagagcg aataacagcg gacgcaatcc
    24061 gaactggacc agctatgaaa aactgcgcac ggttatcgag aagaaaatgt tctccaatac
    24121 cgaggagctg ttgccggtta tttcgtttaa cgccaaaacg tcaaccgacg agcaaaagaa
    24181 acacgacgat tttgtcgacc gtatgatgga aaaaggctac acccgtaaac aggtgcgttt
    24241 actgtgcgaa tggtatttgc gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca
    24301 tgctaccggg cctacaacga cagcgaaccg tgggccagag aagcggcaac acaggcgtag
    24361 catacagttg gcaaatgtag tacggggggc atatgacctg gtttattgac cggcgtctga
    24421 acggcaaaaa caaaagcatg gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa
    24481 ttaaacagtc gatctccgag gccattaata agcgttcggt gactgacgtc gacagcggcg
    24541 agtctgtatc cattcccacg gaagatatta gcgaaccgat gtttcatcag gggcgtggcg
    24601 gtctgcgcca ccgcgtgcat ccgggcaatg accatttcgt ccagaatgac cgaattgaac
    24661 gtccccaggg tggcggcgga ggttccggca gtggtcaggg ccaggccagc caggatggtg
    24721 aaggtcagga tgaatttgtc tttcagattt cgaaagatga gtatcttgat ctgctctttg
    24781 aagatttggc cttaccgaat ctgaaacaaa accaacaacg ccagctgacc gaatataaaa
    24841 cgcatcgtgc gggttatacc gctaacggcg ttccggccaa tatcagtgtt gtgcgttcat
    24901 tgcagaactc actggcacga cgcacagcca tgacggcggg caagcggcgg gaacttcatg
    24961 cgctggaaga aaatttggcc atcatcagca acagtgaacc tgcgcaacta ctggaagagg
    25021 aacgtctgcg caaagaaatt gcagaattac gtgccaaaat tgaacgcgtc cctttcattg
    25081 acaccttcga tttacgttac aagaactacg agaagcggcc cgatccctcc agccaggcag
    25141 tgatgttttg cctgatggac gtttccggtt caatggatca atccactaaa gatatggcta
    25201 agcgttttta tattctgctg tatctgttcc ttagcagaac gtataagaac gtggaagtcg
    25261 tatacatccg ccatcatacc caggcgaaag aagtcgatga acatgaattt ttctactcgc
    25321 aggaaactgg cggcaccatt gtttccagcg ccctgaaact gatggatgag gtagtgaaag
    25381 agcgttataa cccggcacag tggaatattt acgcggcaca agcatcggac ggcgataact
    25441 gggccgatga ctctccgctt tgccatgaaa tcctggcgaa aaaattatta cccgttgttc
    25501 gttattacag ctatatcgaa attacccgtc gcgctcatca gacactgtgg cgagaatatg
    25561 agcatctgca atctactttc gacaactttg cgatgcagca tatccgcgac caggatgata
    25621 tttatccggt gttccgtgaa ctgtttcata aacaaaatgc aacagctaaa gactaaaact
    25681 atcagccggg tcattatcgc ctggctgatt tttagctcac tgtaaattat ctcatttatt
    25741 acatacatta gcttacaatc gctttaaata tgacagcata acctttacac aatttagttc
    25801 cagaaaacaa tcattcggaa aaatgattcg gtcaacacgt gtttccctgg ggttattctt
    25861 taaatatttt ttatcgttaa cgaaaattga tcttggtcaa aactatatat ctctgccatc
    25921 aataaaatcc agcactcaca ttgctctcct ttttatggct tctatgggta cacaaaaatt
    25981 aaaagctcaa ggctttttta ttttcagctt attgctgacg ttaattttat tttgcattac
    26041 taccttatat aacgaaaaca cgaatgtaaa actcatccca cagatgaatt acctgatggt
    26101 tgttgtggct ttgtttttcc ttaacgccgt catttttctt ttcatgttaa tgaagtattt
    26161 cactaacaaa caaattttac caacactcat tttaagcctt gcatttttaa gtggccttat
    26221 ctatttagtt gaaaccattg taattatcca taaaccaatc aacggcagta cactgatcca
    26281 gacaaagtcg aatgatgttt ctattttcta tattttccgc caactcagtt tcatttgttt
    26341 aacctcgctg gcgctctttt gttatgggaa agacaacatc cttgacaaca ataagaaaaa
    26401 aactggcatt ctgttactgg cgctgattcc ttttttagtt tttccccttc tggcacacaa
    26461 tctgagcagt tataacgctg actattcttt gtatgtcgtc gattactgtc cggacaacca
    26521 tactgcgacc tggggaatca actatacaaa aatattggtt tgtctgtggg catttttact
    26581 gttctttatt atcatgcgta cacgattagc cagcgaacta tggccgttaa tagcattatt
    26641 atgtctggca tcgctatgct gcaacttact tctactaact ctggatgagt ataattacac
    26701 catctggtat atcagtcgcg ggattgaagt ctccagtaaa ctgtttgttg tgtcttttct
    26761 gatttataac atttttcagg aactgcaact ctccagcaaa ctggcagttc atgatgtgct
    26821 gaccaatatt tataatcggc gctacttttt caacagcgta gagtcattat tgtcgcgacc
    26881 tgttgttaag gacttctgtg tcatgctggt tgatattaat cagttcaaac gcatcaatgc
    26941 ccaatgggga catcgtgtgg gtgataaagt gctggtttca attgtcgata ttatccagca
    27001 aagcatccgc cccgatgata ttttagcgcg actggagggt gaggtgtttg gcttgctatt
    27061 taccgaactc aatagtgccc aggcaaaaat cattgcggaa cgtatgcgta aaaatgtcga
    27121 actcctgacc ggctttagta acagatatga tgttcctgag caaatgacca tcagtattgg
    27181 cacggttttt tcaacgggtg acacacgtaa tatctcgctt gtcatgacgg aagcagataa
    27241 agccttacgc gaagcgaaaa gcgagggggg caacaaagtg attattcatc atatttaagt
    27301 gcaaaaatat tcagagccat gctttttgcg tggcttttgc atacaattta ttactattac
    27361 ccctaaattt cccctcccca gcggcgcaga gatgagtata attagcgccc ctgtgccagg
    27421 ccgcaatcga actttatctg gttttctcgt ttcactaacc gaaggagtgc catttatcat
    27481 gaaattgcac catagaatgc tccggcattt tatcgccgca agtgtcattg tgctgacatc
    27541 ttccttcctt atttttgaac ttgtcgccag tgacagagca atgagtgcct atctgcgcta
    27601 tatcgtgcag agagcagact cctcctttct ttatgataag tatcagaatc agagtattgc
    27661 cgcgcatgtg atgcgcgctc tcgctgctga gcagtcggaa gtgtcgccag aacagcggcg
    27721 caccatctgc gaggcttttg agtctgcaaa taatacccat ggcttaaacc tgactgccca
    27781 taaataccct ggcttacgcg gcacactaca aaccgcatcc actgactgcg acacaattgt
    27841 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
    27901 tgattacggt tcaggtcttg ggatggccga agagaaattt cactattatc tcgatctcaa
    27961 tgaccgctat gtctattttt atgagccggt taatgttgag tactttgcga tgaataactg
    28021 gtctttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
    28081 cgggcgtacc gttttatcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
    28141 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
    28201 caaaaacaat ctgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
    28261 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
    28321 taatctgttc cagtatgtca gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt
    28381 atccattgat attctttact ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt
    28441 gacggcgtta attttgctga atatggtgcg gatgcacttc cgtttatacc aaaatgtgtc
    28501 gcgagaaaat attagtgatg cgatgacagg actgtataat cgcaaaattt taacccctga
    28561 actggagcag cggttgcaaa aactggtgca atccggttct tcggtgatgt ttattgcaat
    28621 tgacatggac aagttaaagc aaataaatga caccctcggt catcaggagg gggatttagc
    28681 gattacgtta ttagctcagg cgattaaaca gtcgattcgt aaaagtgatt atgccatccg
    28741 actcggtggc gatgaattct gcatcattct agtcgattcg acgccgcaaa ttgcagcaca
    28801 actgcctgaa cgtatcgaaa aacgtctgca acatatcgcg ccgcagaaag agatcggctt
    28861 ctcttccggt atttacgcga tgaaagaaaa cgatacgtta catgatgcgt ataaagcttc
    28921 cgatgagcgt ttatatgtca ataagcagaa caaaaacagc cgttcatgat aaccttctat
    28981 ggttgtttgc ttgtaatctc aggagcatga aatgactgaa atggctaaag gaagcgtgac
    29041 gcatcagcgg ttaatcgctt tattatcaca agaaggtgct gacttccgcg tcgttaccca
    29101 tgaagcggta ggtaaatgtg aagcggtgtc agaaattcgt ggcaccgctt taggccaggg
    29161 ggctaaggca ctggtatgta aagtcaaagg caatggtgta aatcaacatg ttctggcgat
    29221 cctcgcggcc gatcaacagg ccgatctgag ccaactcgcc agccatatag gcggattacg
    29281 cgcttcgttg gccagcccgg cggaagtcga tgaactcaca ggctgtgtct tcggcgcgat
    29341 cccccctttc agcttccatc caaaactcaa actggttgcc gaccccctac tcttcgaacg
    29401 atttgatgaa atcgccttca atgcaggcat gctggataaa tccgttattt tgaaaaccgc
    29461 ggattatttg cgcattgcgc aaccagaact ggtcaatttc cgccgcactg cgtaactagc
    29521 ctgtccgttc gataagaaga acggacaaaa ccagcacaac agcaatggca aaaaacgatg
    29581 acgtgataat cagagtctcg acgaacattt gatcgttcat agtatgcccc cgggagatag
    29641 ctgttttcac gttattgtta gcgtgcgcaa atggcagttt gatgacagat cgcctgattt
    29701 tttatgcaaa aaacgtaaag attttctact tccttcctgc agcaagcgta aagtaagcag
    29761 gcttattatt ctttggcaag gaaaccacga tgtttgatgt cactttgctg atcctgctcg
    29821 ggttagctgc gctgggcttt atcagtcata acaccactgt cgccgtttca attctggtgt
    29881 taatcattgt ccgcgtcaca ccgttaagca cctttttccc gtggattgaa aaacaggggc
    29941 taagtatcgg gataatcatt ctgacgattg gcgttatggc ccccatcgcc agcggcacgc
    30001 taccgccctc aaccctgatt cactcgtttc tgaactggaa atcgctggtg gcaattgcag
    30061 taggcgttat tgtctcctgg ctaggtgggc gcggcgtgac gttaatgggc agccagccgc
    30121 aactggtcgc cgggctgctg gtgggcactg ttttgggcgt ggcgctgttt cgcggcgtac
    30181 cggttggccc gcttattgcc gcgggtctgg tttcgctgat tgtggggaaa cagtagttaa
    30241 tcccgcaata tatcggcctg gcgtttgccc caggcctttc ttaaacatag taataaaggc
    30301 cgttgttgaa tcatacccca gcgtatgagc aactttctgt acagtatcgc ctttcactaa
    30361 tccctgaagc gccataatca gctgtaactg ctgacgccac tggcgaaaac tcaaccccgt
    30421 ctctttcact atcagtcggg caagattgcg ttcactcatc gcaaaaaagc gagcccattg
    30481 ccccaatgac ccccactcga cgggctcttt cgccatcatc tccaccatcg cgcgaatttt
    30541 aggatgagaa gaaacgggta ggtgcagctg ttgttgtggc tgctgaggga gttcatcaaa
    30601 caggacctga attaatctgc gggtcatcgg ctcagcacgc tgagtcgacg ttctgtttgc
    30661 cagggttaaa attaattccc ggcataaggg cgatattttc aatgtgcaac aggttgtcgg
    30721 cattgtcact gcgctgggtt cgataaacag aaaacagagt tcagcgttag ctgtcacttg
    30781 attgctatgt tccaccccgc ccggtatcca aaccgcatat tgcggtggca ccatccacaa
    30841 agcattttcc accgtacagg taattgcacc atgtagcgcc agtatcagct gtcctttgcg
    30901 gtgctgatgt acaggactgg ttaactcatc cgttccggcg tgaatacaaa atgcgaccgc
    30961 ggcttcatgg tgtcggtcgg gttcatagcc attgagattc aacctgtgca tcattttgtc
    31021 cgaatttagc gataatttgt cattttagct tgattcaaca taacaataaa aacggtaagg
    31081 tacagcctcg tttgtaacaa tgagaagcat atgacctgtt caacttcatt aagcggcaaa
    31141 aacaggattg tccttatcgc tggcattcta atgattgcca caacattacg cgtcaccttt
    31201 accggcgcag caccgttact ggatacgatt cgttccgctt actcgctgac gacggcgcaa
    31261 accggcttat tgaccaccct gccattatta gcctttgcgc taatctcacc tctggctgcc
    31321 ccggtggcgc gacgttttgg tatggaacgt agcctgtttg ccgcgttact tttgatctgt
    31381 gctggcatcg caattcgctc tctcccttcg ccttacttct tatttggcgg tacagcggtc
    31441 attggcggtg ggattgcatt aggcaatgtc ttactgccag gattaattaa acgcgatttc
    31501 cctcattccg tcgccagact taccggcgca tattccctga caatgggagc tgcagcggca
    31561 ctgggatcgg ctatggtcgt gccgctggct ttgaacggtt ttggctggca aggcgcgttg
    31621 ctcatgctga tgtgttttcc tctgctggct ctttttttat ggctgccaca gtggcgaagt
    31681 caacaacatg caaatttgag tacctcacgc gccttacata ctcggggtat ctggcgttct
    31741 ccgcttgcct ggcaggtaac attgtttctt gggatcaact cactggtcta ttacgtgatt
    31801 attggctggc ttccggcgat cctcatcagt cacggctata gcgaagcaca ggcgggttca
    31861 ctgcatggtt tgctgcaact agccacagca gctcccggtt tgctgatccc acttttctta
    31921 catcatgtga aagatcagcg tggtattgca gcgttcgttg ccttgatgtg cgcagtgggc
    31981 gcggttgggc tctgctttat gccagcgcac gcgatcacct ggactctgct tttcggtttt
    32041 ggttccggcg caacaatgat actggggttg acgttcattg gtcttcgggc aagttctgcg
    32101 catcaggcgg cggcactctc ggggatggca caatccgtcg ggtatttgct ggcagcctgt
    32161 gggccgccgc tgatgggtaa aatacacgat actaacggtg actggtccat accattgatg
    32221 ggtgttgcca tactttcact actgatggcg attttcggac tttgcgccgg gagagacaaa
    32281 gaaattcgct aatatccggt gctatagtga cgtgaccaat catgcgtgga agggagaaca
    32341 aacacgatga atattcagtg caaacgcgtt tatgatccgg cagaacagag cgatggttat
    32401 cgcgtactgg tcgaccgcct ctggccgcgt ggtatcaaaa aaaccgattt agcccttgat
    32461 gagtgggata aagaaatcac gccgtcaacg gaattgcgca aagcctttca cggcgaagtc
    32521 gtcgattttg caacctttcg cgagcaatat cttgcagaac tggcgcaaca cgagcaagaa
    32581 ggaaagcggc tggcggacat cgccaaaaaa cagccgctga ccctgctcta ctcagcaaaa
    32641 aacaccacgc agaaccatgc gctggtgctg gccgactggc tacgtagctt gtgattttag
    32701 tacagcatcc ggcggttatt tttcaccagc cggatggtcc cgtcgccaca atgcccattc
    32761 atcaatcgtt tcaccgcccg gtaatttgca attgttgctg accccttgcg ctgtctgcac
    32821 tggaatgagc gtcccgccct tctgctggca atagaccgac gccggatttg ctataccaat
    32881 ctgcggcggt ttgggtgctt ctggctgaga aggggttgaa caaccagcca ggaccagcaa
    32941 gcaaggcaga acaaaactga taattttcat ttattgatct cacatattta tccagactta
    33001 gagtatcgcg gtatcgtttt gttttgcagc actattttta ttacattcac tcaaatcata
    33061 ttacgccttg tttcatcttt gttgatgatg ttttatcatg cctgcaaaga ttaaataatc
    33121 agcatttacc cgccgtatcc tggagttgtt tcgtgtcaga tcagattatc gcccgcgtct
    33181 cgcaatccct tgccaaagaa cagtcactgg aaagtctggt ccgacagctt ctggagatgc
    33241 tggaaatggt cactgatatg gaatcaacct acctgaccaa agtagatgtc gaagcgcgcc
    33301 tgcagcatat tatgtttgcc cgtaacagcc agaaaatgca catcccggag aattttaccg
    33361 tctcgtggga ttactcgtta tgcaaacgcg ccattgatga aaactgcttt ttcagcgatg
    33421 aagtccctga ccgttggggc gactgtattg cggcacgcaa tcttggcatc accacatttc
    33481 tgagcacgcc aattcactta ccggatggat cattctatgg cacgctttgc gccgccagta
    33541 gtgagaagcg ccagtggagt gaacgcgcgg aacaggtttt acagttattc gccggactga
    33601 ttgcacaata tattcaaaaa gaggcactgg ttgaacagct gcgcgaagcc aatgctgcgc
    33661 tgattgcgca atcgtatacc gactcgttaa ccgggctacc gaatcggcgg gcgatttttg
    33721 aaaacctgac gacgctgttt tctctcgccc gacatcttaa ccataagata atgatcgcgt
    33781 ttatcgatct ggataacttc aaattaatca acgatcgttt tggtcataat agtggtgatt
    33841 tgttcctcat tcaggttggc gagcgcctta atacgctcca gcaaaatggc gaagttattg
    33901 gtcgtctcgg cggtgatgag tttttggttg tttcactgaa caacgagaat gcggatattt
    33961 cgtctctgcg agaacgtatt cagcagcaaa tacgcggaga atatcactta ggtgatgttg
    34021 atttgtatta tcccggtgcc agtcttggca tagtagaagt cgatcctgaa acgaccgatg
    34081 cagacagtgc cctgcatgct gccgatatcg cgatgtatca ggagaaaaaa cacaaacaga
    34141 aaacaccttt tgtcgcgcat ccagcgctac attcctgagg cgtattcaca tccttttgat
    34201 tggtgataac atgcgaatcg gtattatttt tccggttgta atctttatta cagcggtcgt
    34261 atttttagca tggtttttta ttggcggcta tgctgccccg ggagcataaa gatgaaaaaa
    34321 acaacgatta ttatgatggg tgtggcgatt attgtcgtac tcggcactga gctgggatgg
    34381 tggtaacgtc acatctagaa aatagcaaag gctgcctgtg tgcagccttt gtgcaattta
    34441 cgcgttaact tttaatcttt ctgtagataa acagcacgac aatcgcgcca ataacggcaa
    34501 ccacgaagct gccaaaattg aagccatcga ctttaccaaa gccaaacagc gtgctgatcc
    34561 atccgccgac tacggcaccg actatcccca gcaggatagt cataaagaat ccaccaccat
    34621 ctttacctgg catgatccac ttcgccagaa taccggcaat aagcccaaaa ataatccatg
    34681 acagaatgcc cattgtttcc tcacttatct gttttgcatt agcgggttag tcgctgataa
    34741 aaagcatagc acaacatcgg gagggcaaga tttgtgacga gcatcacgga ggattttttt
    34801 gcgatggcgc agaaattgcg ccatcaacga tcagtgataa ttaccaacca caaacatcat
    34861 gttcgttttc cgtgtcataa gaacgcacgg tattcaccag atcttttatc acttcagccg
    34921 ccacttctgg caccagcaaa gtcatcggcg tctctgtttc ataatcgaca gaaacgccat
    34981 tgctgttatt ggtgacggtc acggtatacg ttgctttgcc catgattcat ttcccgttat
    35041 gaatgacttt ccgttgttgc gcaccttcca tcaggacttc aggggcaacg aagaagtcaa
    35101 tgttgaaata agtatcgtca gtcatggctt caatgttgtg ccacttttct ggagggaaca
    35161 ccgcaaactg ccccgcttcg ataaggatca cctgatcagg ctctgcactg tgttcatcag
    35221 ggtagccgag atatttgacc gccccatgca taacggaaag gcgtgggtaa actcccgggc
    35281 gcgttccttt atcaagatga cgttcgaata ttccggcagg cgcagtttgt ttattccaga
    35341 aaggcgttga gcgcgtatga atataattct gtgggatttg cagcatcctt ttccctcctt
    35401 cggtgaatgc gctgaaaatg gtttattcca gccgtttcag ggtacgcctg ataatttgca
    35461 ttttaaatac catttattgg ttacttttta gcaccatatc agagaagaat cagggaggat
    35521 tacagatggg aagcccgcga caacgcgggc tgaaagcatc aggattgcag cgtcgccagt
    35581 cgggcggcga aacccacgaa catcaaacca atcagtgagt tgcccacttt agccagtttc
    35641 tttttggtac gtatgtactg tgtgacaaaa gcaccagaaa taatcaggaa gctcaaatag
    35701 cagaaactca ccagttccag cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg
    35761 gcattaacat cgataaactg tacgaaaaac gacacataga acaaaatggc tttcggatta
    35821 gtcaggctca aaattaacgc gcgtttaaaa atggcaccgt attgaggctc atcggatttg
    35881 gcctcgttat ttttaccctt cagggtcgcg tagagaattt tactccccag atagagcaaa
    35941 taaaacgcac caagataacg tacaatgttg aataatatcg gggtggtctt aattaatgtc
    36001 gccactccag cccatgccag aaacatcaat accgcatcgc caataaatac accgcaggcc
    36061 gcaagataac cgcctttcat accgctactg acgctatttt tgagtacaaa cagggtattt
    36121 ggccctggca ccaacacaat aaaaatggcc ccaaccagat aggtccagta attcagaacc
    36181 ccgtattcag cgaacacatt aacctcttta attatctttc gatcatgcgc gattaaaggt
    36241 gaatatgcta accaatctgt agcggcttag aaaggagaaa atcaggtttt aacccgatat
    36301 caacccgata attgaatcat taatcaggca tgctccagtg aaaaattcgg gtagtgctcg
    36361 gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt cgccagcctt
    36421 gaaacataaa cggcccagac gttcgctggc tggtaatatt ccggtagcac ttgtactaaa
    36481 tgaccactgg caatgttttc gctaacatcc caccaggagc gcagagcaat cccctgccca
    36541 tcgagacacc actgatggac aatttctcca tgattagacg acagtgggcc ggtaacttta
    36601 atcgcatggt ggccttcttt attgcgtaat tgccagacac caaaaggatg atcgcgctct
    36661 ttaatgacca ggcacggcaa tgcagaaaga tcggttaaat gtttcggcgc gccatgttgc
    36721 gcaataaatt ccggcgacgc gcagagaatg cggtaattgg tcgccagttt gcgggcgatt
    36781 aaattagggg cgatatcatc gccaatgcga atatcgagat cgacaccttc attgaccaaa
    36841 tcgaccaacc ggtcttccac atcaaaacgt aattccagtt gcggatacgc cttcgccagc
    36901 gctgataacg ccggagccac tacctgtcgt ccaaaaccaa agctgctgat aatacgcagc
    36961 atcccctgcg gcacctgacg cacgtcagaa agttcgtcca tcatttgacc gacatcctgc
    37021 aaaatccgct gcgcccattc ataaatccgc tctccttctt cggtaatggt gacgcggcgg
    37081 gtggtgcggt gtaacaacac cacgtttagc gtttgctcca gcaaggcgat gcgcttgctg
    37141 acgaacgccg gagaaacgcc cagttcttcc gccacggcgg caaaaccagc ccggcgagcc
    37201 accagcataa agacgcgcaa atcgttcagc agcggtaaat tattcatgat tcgtgtttta
    37261 tgtttcacca gttacaggga ttaattcctt ttcagtcaat tataggatgg tgatgttgtc
    37321 aattttgatg gtcaggaagt gagaacccaa tgatgaaaac gatgcgtatt gctgcgatcc
    37381 cgggagacgg gattggcaaa gaagtccttc ctgaagggat tcgcgtgtta caggctgccg
    37441 ctgagcgctg gggcttcgcc ttgagttttg agcaaatgga gtgggcgagc tgcgagtatt
    37501 acagccatca cggtaaaatg atgcccgacg actggcatga gcaacttagc cgtttcgacg
    37561 ccatctattt tggtgccgtc ggctggccgg acaccgttcc ggaccatatt tcgttgtggg
    37621 gttcgctgct gaaatttcgt cgtgaattcg accagtacgt caacctgcgc ccggttcgtc
    37681 tctttcctgg cgttccctgc ccgctggcgg gaaaacagcc tggcgacatc gatttttacg
    37741 tggtcaggga aaacaccgaa ggcgaatatt cctcgctcgg cggcagagtg aatgaaggta
    37801 cagagcatga agtcgtcatt caggaatcgg tatttacgcg tcgtggtgtc gatcgcattt
    37861 tgcgttatgc cttcgaactt gcgcaaagcc gcccacgtaa gacgctaact tctgccacta
    37921 aatcgaacgg tttagccatc agcatgccgt actgggatga gcgagtggaa gcaatggctg
    37981 agaattaccc ggagatccgc tgggacaagc agcatattga cattctctgc gcacgttttg
    38041 tgatgcagcc ggaacgcttc gatgtggtag tggcgtccaa tttgtttggc gatatccttt
    38101 ccgatcttgg cccggcctgc accggcacca ttggcattgc cccatccgcc aacctgaatc
    38161 cggaacgcac tttcccgtcg ctcttcgagc cagtccacgg ttccgcgccg gatatctacg
    38221 ggaaaaacat tgctaaccct atcgccacaa tttgggccgg tgcaatgatg ctcgattttc
    38281 tcggcaatgg cgatgagcgt ttccagcaag cgcataacgg tattctggcc gcgattgaag
    38341 aagtgattgc tcacgggccg aaaacacctg atatgaaagg cagtgccacc acgccacagg
    38401 ttgccgacgc gatttgcaaa attattttgc gttaaggtca aaccagttta tttgaaccgc
    38461 gtcactgacg cggttttttt attcgttctt tgcagtaaat aacctgcgtc atttcacctt
    38521 ttattgtttc cgtttcgtgt tttatggctt tccgtattct taattgttta atttatgtaa
    38581 catgcaaata atcaatttgt taaacacaac atacaactca ttaccctgca aataattaca
    38641 actggcaaaa gccagagacg ggcttttatg cccaggagat gactcatgat gagcaatgtt
    38701 aagaaaaaag atgtgccgct gataagcatc agcctggtgg ccattctttt catcgcagct
    38761 gcattaagcc ttttcccaca acaatcggcc gacgcggcca acgcaatata cacttttgtt
    38821 acgcgtacgt taggttccgc cgtacaggta ttggttttgc tggcaatggg gctggtgatt
    38881 tatttagcca ccagtaaata cggcaatatt cgtcttggcg aaggaaaacc ggaatacagc
    38941 acgctctcct ggctgtttat gtttatttgt gccggtttag gttcttctac gctttattgg
    39001 ggggttgctg aatgggccta ttattatcaa acgcctggat taaatatcgc accgcgttca
    39061 caacaggcac tcgaatttag cgttccctac tccttcttcc actggggcat cagcgcctgg
    39121 gcaacttata cgctggcctc attaatcatg gcttatcact ttcatgtgcg gaaaaacaaa
    39181 gggctgagcc tttccggcat tattgctgct attaccggcg ttcgcccgca aggcccgtgg
    39241 gggaaactgg tcgatttgat gttcctgatc gccactgtcg gcgcactgac catttccctt
    39301 gttgttaccg cagcaacctt tacccgtggg ctttccgcgc tgaccggttt acccgataac
    39361 tttaccgtac aggcatttgt gatcctgctt tccggcggca ttttttgcct aagctcgtgg
    39421 attggtatca acaacggttt gcaacgtcta agcaaaatgg ttggctgggg cgcgttcctg
    39481 ctgccattac tggtgctgat tgtcggccca accgaattta ttaccaacag catcatcaat
    39541 gccatcggcc tgaccacgca aaacttcctg caaatgagct tattcaccga tccgcttggc
    39601 gatggttcat ttacccgcaa ctggaccgtt ttctactggc tgtggtggat ctcttacaca
    39661 cctggcgtag caatgtttgt cacccgcgtt tcccgcggtc gtaagattaa agaagttatc
    39721 tggggactga tcctcggcag caccgtcggt tgctggttct tcttcggcgt aatggaaagc
    39781 tatgccattc accagtttat caatggcgta atcaacgtcc cacaggtgct ggaaacactg
    39841 ggcggcgaga cagctgtgca gcaagttctg atgtcgttgc cagccggtaa attgttcctc
    39901 gccgcatacc tgggcgtgat gattattttc cttgcctcgc atatggatgc ggtggcctac
    39961 accatggcgg caaccagtac gcgtaatctc caggaaggtg acgatcctga ccgtgggctg
    40021 cgtcttttct ggtgcgtggt gatcactctg atcccgcttt ccatcttgtt taccggtgct
    40081 tcgctggaaa cgatgaaaac caccgtcgtg ctcacagccc ttcccttcct cgtcatttta
    40141 ctggtgaaag tcggtggatt tattcgctgg ctgaaacagg attacgccga cattccggct
    40201 catcaagttg aacattatct cccgcagaca ccggttgaag ccccggaaaa aacgccagtg
    40261 ctccctgcgg gaaccgtatt caaaggcgac aactgagcgc catcatccta acgataaagg
    40321 taaccctatg agcaatctga gccctgactt tgtactaccc gaaaattttt gcgctaaccc
    40381 gcaagaggcg tggaccattc ctgcccgttt ttataccgat cagaacgcct ttgaacacga
    40441 aaaagagaac gtcttcgcca aaagctggat ttgcgtcgct catagcagcg aactggcgaa
    40501 tgccaatgat tatgtgacgc gtgagatcat tggcgaaagc atcgtgctgg tacgtggtcg
    40561 tgataaggtt ttgcgcgcgt tctataacgt gtgtccgcac cgtggtcatc agttgttgag
    40621 cggtgaagga aaagcaaaaa atgtgattac atgcccgtat cacgcatggg cattcaaact
    40681 cgatggcaac ctggcccatg cacgtaactg cgaaaacgtc gccaatttcg atagcgacaa
    40741 agcgcaactg gttccggtgc gtctggaaga atatgcagga ttcgtcttca tcaacatgga
    40801 ccctaacgcc accagcgtag aagagcagtt acccggtctg ggcgcgaaag tgctggaagc
    40861 ctgcccggaa gtccacgatc tgaaactggc ggcccgcttt accaccctca cgcctgccaa
    40921 ctggaagaac attgtcgata actatctcga gtgctatcac tgtggtccgg cgcatccagg
    40981 tttctccgac tccgtacagg ttgatcgtta ctggcacacc atgcacggta actggacgct
    41041 gcaatacggt ttcgccaaac cgtcagaaca gtctttcaaa ttcgaagagg gcacggatgc
    41101 ggcattccac ggtttctggc tgtggccgtg cacgatgctg aacgtcaccc caatcaaagg
    41161 gatgatgacg gtcatttatg aattcccggt ggattctgaa actaccctgc aaaactacga
    41221 tatttacttc accaatgaag agttaaccga cgagcaaaaa tcgctgattg agtggtatcg
    41281 cgatgtgttc cgtccggaag atttacgtct ggttgaaagc gtacagaaag ggctgaaatc
    41341 gcgtggctat cgtggtcagg ggcgcatcat ggccgacagt agcggtagcg gcatttccga
    41401 acatggtatc gcccatttcc ataatctgct ggcgcaggta tttaaggact aatgacatcg
    41461 gcggcggtat tttccgccgc tgggctgatt ttgatggagt acagcaatgt cagactatca
    41521 aatgtttgaa gtacaggtga gccaaattga accgcttacc gaacaggtga aacgcttcac
    41581 gctggtggca accgatggca aatcattacc tgcgtttacc ggaggaagtc acgtcattgt
    41641 gcagatgagc gatggtgata acgagtacag caatgcgtat tcactactga gttcgccgca
    41701 tgacacctct tgttatcaga ttgccgttcg gctggaggaa aactcgcgcg gcggttcccg
    41761 ctttttgcat cagcaggtaa aagtgggcga tcggttaacg atttcaacgc ctaataacct
    41821 gtttgcgcta attccctcgg ccagaaagca tctgtttatc gcgggcggca ttggtatcac
    41881 ccctttcctg tcgcacatgg cagagttgca acacagcgac gtcgactggc agctacatta
    41941 ctgcacgcga aatccagaaa gttgcgcatt tcgtgatgag ctagtccagc atccgcaggc
    42001 tgagaaagtc catttgcatc attcatcaac cggaacacga ctggaattag cgcgattatt
    42061 ggcggatatc gaacctggca cacatgttta tacctgtggc cccgaggcgc taattgaagc
    42121 ggtaagaagt gaagctgcgc atctggacat cgccgccgat acgctgcact ttgagcaatt
    42181 tgctatcgaa gacaaaaccg gcgatgcatt taccctggtg cttgcccgtt ccggtaaaga
    42241 gtttgtggtg ccggaagaga tgactatttt gcaggttatt gaaaataata aagcagcgaa
    42301 agtggaatgt ttatgtcgtg aaggggtatg cggaacctgc gaaacggcaa tactggaagg
    42361 tgaagctgac catcgggatc agtattttag cgatgaagag cgtgctagcc agcaaagtat
    42421 gttgatctgt tgttcgcgtg cgaagggtaa acgcctggtg ttggatttgt agtttgccga
    42481 agccggactg agcgcgccca gtccggcttc ggaagatttt actgcggata ttcctgcaat
    42541 aaattgtgta atgcttccgc catcagctca ccacgccagc cggaaatcag ctccggcaaa
    42601 ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat
    42661 gccagcaatt cggcgctgat cttgtgcgtt tcgctcacgt cagtaatcag cgacttaatc
    42721 gctttaaacg ctttacgata acccggcatg tccatcaggt taagcatcgg ctgcggtaag
    42781 gcctcttccg gcaatgtctg cgctttttcc accagcgcca gcagcgtttt accgtgaaag
    42841 cggatttcgc taccggataa gcccaggctg tccagttcgc ctaaacttcc cggcatataa
    42901 cgcgctaccg accacaaatg ctcttcacgc acgacaaagt tcaccgccag atcgcgctcc
    42961 cgcgccttgc gcagtcgcca gtcggctaac agttgcagac aggccagttg gcgtgtgcgt
    43021 aactgccagg cattggtaat atcacgccag gcatcttccg gtgcaacgac ttcctgacga
    43081 cgcatttgca tcaggcggca ttcatccagc gccgcaggta gccagccgga ggcctccgtt
    43141 tctaccataa gctttgcggt gatcggtaac agataccaga catccgccgc tgcgtattca
    43201 cactgacgtt cggtcagcgg tctggccagc cagtcggtgc gcgattcact cttgtccagc
    43261 gtaacgccgg aatactcttc caccatggaa gcgaaacccc atgacatcgg gcgtccgcag
    43321 aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa gacattgagg
    43381 aacacttcca gatcttcact gcccgcatgg agaaattttg tgatggacgg atcgcgcagg
    43441 atcgctttca gcggtgacca gtcggtgatc ccgagaggat caattagcgc cagatgctcg
    43501 ccatcgaaaa gttgaattag acccagttgc gggtaatagg tgcgcgtacg aacaaattca
    43561 gtatccaggg ctatcgccgg aaaggcacgg acggcttcac acaaagaagc cagcgcatcg
    43621 tccgtggtaa tcatttggta attcaaattg tattctcttt agtgggcgtc aaaaaaaacg
    43681 ccggattaac cggcgtctga cgactgactt aacgctcagg ctttattgtc cactttgccg
    43741 cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga cgttagattt cggtaactca
    43801 tcacgaaact ccaccagctt cggtactttg tagcccgtga gctgacggcg gcaaaaagtc
    43861 accagtgact cttcggtaag cgatggatct tttttcacta cgaagatttt caccgcttca
    43921 ccactggagc cggaaggtac gccaacagcc gcgacttcct gtacgccagg atgctgcatg
    43981 acgacatctt caatctcgtt gggatagacg ttaaaaccgg aaaccagaat catgtctttt
    44041 ttgcgatcga caatacgcag gaatccttct tcatccatca ccgcgatgtc gccagtgtgt
    44101 aaccagccat ttttgatgat ctcatcggta gcatccggac gctgccagta acccagcatc
    44161 acctgcggtc ctttgacaca aagctcgccc ggttgacccg gtggtacttc gttgtcgtcg
    44221 tcatccacca gtttggcttc cgtcgatggc accggcaaac cgatgctacc gctatgataa
    44281 tcaatatcat acgggttaac gctgaccagc ggcgcacact cggtcaggcc ataaccttcc
    44341 agcagatact gtccagtcag tttcacccaa cgttctgcca ccacttgctg aacaggcatc
    44401 cctccgcctg cggaaagatg cagactggag aaatccagct gctggaactc tttattgttc
    44461 agcaacgcat tgaacaaggt gttaacgcca gtgatagcgg taaacggata tttcgctaac
    44521 tcttttacca accctggaat atcgcgcggg ttagtgataa gcaggttctg cccacccagt
    44581 tcgataaaca gcaggcagtt aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc
    44641 accaccagct ctttgcccgg atgcaacagc ggaccatagg tcgcgttaac ctgttccagg
    44701 ttcgccagca tattgcggtg agtcagcatc gcgcctttcg ccacaccagt ggtaccgccg
    44761 gtgtattgca ggaaagctaa atcttccggc accagttcgg gtttgacgta ctgcatccgg
    44821 tagccgttgt gcagggcgtt acgaaatgaa atggcatctg gcagatggta tttcggcacc
    44881 agacgcttga tgtatttaac aacgaaattg actaccgtgc cttttgccgt agatagctga
    44941 tcgcccatac gggtcagaat tacgtgctga acggcggttt tatcaaccac tttttccaga
    45001 gtgtgggcaa agttagacac gataacaatc gccgatgcgc cgctatcgtt aagctgatgc
    45061 tcaagttcac gcggggtata caacgggtta acgtttacga caatcatccc ggcacgcaaa
    45121 atgccaaaca gcgccaccgg atattgcaat aaattaggca tcatcaacgc aacgcgatcg
    45181 cctttcttca gtcccaaccc ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc
    45241 agtttgcgga aggtcatcac ctcccccata ttcacaaacg cgggttgatc ggcgtagcgc
    45301 gcgaccgact gctcaaacat atctaccaga gattgataac ggtcagggtt gatctccgtc
    45361 ggaacgtccg cgggataacg gttaagccaa accttcttca attcttcacc tctaaaatgc
    45421 gtgttcgtcg tcatcgcaac cccaaatgat atacatgccg ttaacataat attaactcat
    45481 cataccagct tgataattac ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta
    45541 tctttaccgt aagaatgcag aaacagcgga ccagccgctg tttctttttc tttaaaaaca
    45601 agcaattatt cagttacaac agtttgtact ctcgcggggc cgggattata ccagccccat
    45661 ccaccgtagc catagggcca gccacgaccg ccataaaacc atggatcaat cggctgaggc
    45721 ggcataatca cctgctgggt taaatgccaa cgtttgtaac ccgttacctg catcaccata
    45781 aatttatagg gcgtgttgcc gattttgccg tcaaccgcac cggtgattgg cccaactacc
    45841 gtaaccagtt gtccacggaa atctaccggg tccagaaaac cgttcacatc agcataaatg
    45901 cgaccgcgag aaggttctcc cagcgtcggt ctggctccgc tgtccagtgg tacggtggca
    45961 atttccaggc gggttttccc ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt
    46021 gcctcctgac caacgtacag ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc
    46081 gtgggactgc tgcctttaat ggcgtccggc acagtgacac aaccgctcag cattagcgca
    46141 aacgtacctg ccagtatgcc tttgataacg ttcttttgaa ccgccatgat gcgactcctt
    46201 tttttctcag ggcatactct taagattcat tctttgcccg gaagtttctt ccatgcgacg
    46261 ttgttacgta aataaaccgg ttcggcatgt tctaccgcca ccgttttacc ctcggcaaac
    46321 atctgacaag caatcggcag catatctcct gcagcgggga gtaacacttc gccatcgcgc
    46381 aaaaccagcc cgctctcttt accgagatcc ggccaggctt gccagcccgt ccctaccgtc
    46441 acccattcgc cggaaagctg ttgcattcgt tcatgaacga gttcgggttt gagtacggct
    46501 tcggtttctt caccgtgcca gataccgttt tcatcacgct gatattcggc ccagtaaact
    46561 tcgcccattc gcgcgtcaat ggctgccagc acgcgtgttg cgccgttttt gcgccacgcc
    46621 ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg gtaattccgc gccaagcgcc
    46681 agcccttgcg cgatgccaat accaatgcgc acgccagtaa agctaccggg gccgcgcccg
    46741 taagccagag cgttaatatc agtcagggaa gttccgctgg tggtcaggat atcctgcacc
    46801 atcggtaaga ttcgttgagt atgttcacga gggcaaagct caaaatgagc gttgaccgta
    46861 ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc
    46921 atgggtcttc gtgcttagat caataaaaag gcgcgcatca taccatactc catcacaaat
    46981 tacctggagg atggtatcgc aaggaaacga accgcacggg caatgtcacg ggtgcgcggc
    47041 gctggcggca gactggcgag aaacgtcgcg ccgtaagggc gcatcaccag ccgattatcg
    47101 caaatcacca aaacccctcg atcgtcggcg tcgcgaatca atcgccctac tccctgcttg
    47161 agagtaatga ccgcatccgg cagctgtact tcatcgaacg ggtcgcctcc gcgcaaacga
    47221 caatcttcca tgcgcgcttt taatagtgga tcatccggtg aggtaaacgg caacttgtcg
    47281 ataatcacca gcgacaatgt atcgccacgc acatccaccc cttcccagaa gctgctggtt
    47341 gccacaagaa gcgcgttacc ggcgctgaca aattgctgca acagttgccc tttgctggtt
    47401 tccccctgca acaaaacggg aagcgtcatg gtagcgcgga actgctcagc cagatcgcgc
    47461 atcatggcgt gcgaggtaca aagcataaaa caacgaccgt tgttagcttc gatgatcggt
    47521 cgcagcattg ccgccagttg ccgtgcggaa cccggttgat tagtttgcgg caggttgcgc
    47581 ggcacacaga gtaacgcctg gcggctgtaa tcaaaggggc tgggtaacag caacgactcc
    47641 gcctgttcga tgccaagccg cgaggtgaaa tgatgcagat cgtcattcac cgacagcgtt
    47701 gccgaggtaa aaatccagct acctggcttt tgcgccatta actctttgaa tttatccgcc
    47761 acgctaagcg gcgtcagcgc gagagtgaaa tggcgcgagg tacattcgta ccagtagctg
    47821 taacccggct gattaatctc ttttagccgt ttgagtcgcg tgcgatataa cgtggcgcgt
    47881 tcaaatgccg catccagcaa ggcggaacgc cccagcgaaa gtttcgccac gtcataacaa
    47941 agttccaggg tgtcatcgag cagtaaaaat gcccgctgaa tttgcggatt agctaacagc
    48001 tcacgcaggt taccgcgata accaggctca ccgagttgca ggcgaaaatc ctgcgcactc
    48061 tgggcaagac ggtcagcgca cttttgtaac tgctgggtgt cttttaattc ggtgcggtag
    48121 gcgatggtga tgtcttttgc caggtcgagc agttgtcgac tggagagtga ctgaccaaaa
    48181 tactggctgg caatgtccgg tagctggtgg gcttcgtcga agatcatgac gtccgcttcc
    48241 gggatcagtt cgccaaaacc gctctctttc accaccatat ccgccagaaa gagatgatgg
    48301 tttaccacca ccacatcggc gtccatcgct tttttgcgtg ctttgaccac aaagcaatct
    48361 ttatacatcg ggcagtcgct gccaagacag ttgtcgttgg tgctggtgac cagcggccac
    48421 gcctgtgaat cttccgccac gctgacgcag gtgctgatat caccatcgac tgtttgatta
    48481 gaccaggagc gcagcaggat cacatcgctt aagatttgta ccggcagatc gccccccgcc
    48541 agcgcctgct gttcgagacg ttcgaggcag aggtagtttg agcgcccttt cagcaacgcc
    48601 acattgcccg tgtatttcaa tgcctttgag actgttggca aatcgcggct gtagagctga
    48661 tcctgcaacg cttttgagcc ggtcgagata atgactttct ttttcgcccg cagcgcagga
    48721 gccaggtaag cgtaggtttt gcccgtaccg gttcctgctt ccaccaccag cggctggcct
    48781 ttttctatcg cctgggtgac ggctaccgcc atctgtcgct gtggttctcg cggcttaaag
    48841 cctggtatcg ctttcgccag ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct
    48901 gttgatttga acagggatta tgtcaggatg agggcgcttt cgccagttga agtgatgacg
    48961 gcgacctcac attgtggcag tctttgcagc acgaaaatgg aagtataatg aggacaaaat
    49021 gatgactatc gttcgtatcg atgctgaagc ccgctggtct gatgtggtga tccacaacaa
    49081 cacgctctac tacactggtg taccggaaaa cctcgacgcc gatgctttcg agcaaactgc
    49141 caacacgctg gcgcagattg acgccgtgct ggaaaaacag ggcagcaata aatcgagcat
    49201 tctggatgcc accattttcc tggccgataa aaacgacttc gcggcgatga ataaagcgtg
    49261 ggatgcctgg gttgtcgcgg gtcatgcgcc ggtgcgctgc acggtacaag cgggtttgat
    49321 gaacccgaag tataaagttg aaattaagat tgtggctgcg gtgtaagctt tatcgaagca
    49381 aaataagtca gacgataatt tatcgataat actggtcggt tttacataaa tcgaccagag
    49441 attaagatat tactgttgat gctcctgcta attcattatt tatttttata ttttcaattt
    49501 tagctataaa gctaactata taaataaaca taaccatgcc gttgtgttcg cccttcgatt
    49561 agcatctctg gttttctttc ttagctgaac aatttggtta tagcgtgaga actgaccgtc
    49621 aattttctct ctcttactgg ataacacacc atgagctgct acctttaaaa cacctgaatg
    49681 tcaacaggtt aactcgtgcc atatcgttat aatccctttg atattagatg caaattaagg
    49741 tcatatatag ccttatcgat aacatggtta attttaagga taagaatatg cctgctgtaa
    49801 tagataaagc cctggatttc attggtgcca tggatgtatc agcaccaaca ccaagttcga
    49861 tgaatgaaag cacggcgaag ggaatcttta aatatttaaa agaactggga gtacccgcga
    49921 gtgccgctga tattacggcg cgagctgacc aggaaggctg gaacccgggg ttcacggaaa
    49981 aaatggttgg atgggcaaaa aaaatggagt ctggtgaacg cattgtgatt aaaaatcctg
    50041 aatacttttc aacatatatg caggaagaac taaaagcact ggtctaagtt aaatttatat
    50101 cagcataaat gggtcaggac gcttttaatc acatatgaaa agcgtccttt ttctcacaac
    50161 caaataacca ttgacttcta taacgaaagc gatacggata agtgtgttac tcgtcttcat
    50221 cctcaaaacg cgccacgatt cgctcgccgg aatggttggc acgcagctct tctgccacca
    50281 acgcgattgc ctggccgctg ctcatccctt gtgccatcag ttcctggatc cgctcgacgg
    50341 ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc aaacattgtt aactcctgct
    50401 aaattgttgg cgctaattat ttcatgctac ccggcacata gccagtagag tcaggactga
    50461 tgaagacgtt atctcccgct gtgattactt taccctggcg tcaggacgcc gctgaatttt
    50521 atttctcacg cttaagccac ctgccgtggg cgatgctttt acactccggc tatgccgatc
    50581 atccgtatag ccgctttgat attgtggtcg ccgagccgat ttgcacttta accactttcg
    50641 gtaaagaaac cgttgttagt gaaagcgaaa aacgcacaac gaccactgat gacccgctac
    50701 aggtgctcca gcaggtgctg gatcgcgcag acattcgccc agcgcataac gaagatttgc
    50761 catttcaggg cggcgcgctg gggttgtttg gctacgatct gggccgccgt tttgagtcac
    50821 tgccagaaat tgcgcagcaa gatatcgttc tgccggatat ggcagtgggt atctacgact
    50881 gggcgctggt tgttgaccac cagcgtcaaa cagtttcttt gctgagtcat aatgatgtca
    50941 atacccgtcg ggcctggctg gaaagccagc aattctcccc gcaggaagat ttcgcgctca
    51001 catccgactg gcaatccaat atgacccgcg agcagtacgg cgaaaaattt cgccaggtac
    51061 aggaatatct gcacagtggt gattgctatc aggtgaatct cgcccagcgt tttcatacga
    51121 cctattctgg cgatgaatgg caggcattcc ttcagcttaa tcaggccaac cgcgcgccgt
    51181 ttagcgcttt tttacgtctt gaacagggtg cgattttaag cctttcgcca gagcggttta
    51241 tcctttgtga taatagtgaa atccagaccc gcccgattaa aggcacgcta ccacgcctgc
    51301 ccgatcctca ggaagatagc aaacaagcag aaaaactggc gaactcagcg aaagatcgtg
    51361 ccgaaaatct gatgattgtc gatttaatgc gtaatgatat cggtcgtgtt gccgtagccg
    51421 gttcggtaaa agtaccagag ctgttcgtgg tggaaccctt ccctgccgtg catcatctgg
    51481 tcagcaccat aacggcgcaa ctaccagaac agttacacgc cagcgatctg ctgcgcgcag
    51541 cttttcctgg tggctcaata accggggctc cgaaagtacg ggctatggaa attatcgacg
    51601 aactggaacc gcagcgacgt aatgcctggt gcggcagcat tggctatttg agcttttgcg
    51661 gcaacatgga taccagcatt actatccgca ctctgactgc cattaacgga caaatatact
    51721 gctctgcggg cggtggaatt gtcgccgata gccaggaaga agcggaatat caggaaactt
    51781 ttgataaagt taataagata ttacgccaac tggagaagta agacgtggaa taccgtagcc
    51841 tgacgcttga tgatttttta tcgcgctttc aacttttgcg cccgcaaatt aaccgggaaa
    51901 ccctaaatca tcgtcaggct gccgtgttaa tccccatcgt ccgtcgaccg caaccggggt
    51961 tgttgctgac tcagcgttcg attcatctgc gtaaacacgc cggacaagtg gcattccctg
    52021 gaggcgcagt cgatgacacg gacgcatcag ttatcgccgc cgcgctgcgc gaagctgaag
    52081 aagaggtcgc tataccgcct tccgccgttg aggttatcgg cgtgctgccg cccgtcgata
    52141 gcgtcactgg ctatcaggta accccagtgg tcggcattat cccgcccgat ctgccgtatc
    52201 gcgccagtga agatgaagtc tcggcggtgt ttgaaatgcc gctcgcccag gcattacatc
    52261 tgggtcgtta tcacccttta gatatctacc gccgtggcga ttcacatcgg gtatggctgt
    52321 cctggtacga acagtatttt gtatggggaa tgaccgcagg cataattcgt gaactggcgc
    52381 tgcaaattgg tgtgaaaccc tgactatact tatctttaca tctacaaaac actacttgag
    52441 acaatcatcg caatattagt taaatcgcgg tttttgatta gtttaattca tgtgaatagt
    52501 taagctagtc gccgcgttcc ctcttacact atgcgctgtt attagttcgt tactggaagt
    52561 ccagtcacct tgtcaggagt attatcgtga ttagtctatt cgacatgttt aaggtgggga
    52621 ttggtccctc atcttcccat accgtagggc ctatgaaggc gggtaaacag ttcgtcgatg
    52681 atctggtcga aaaaggctta ctggatagcg ttactcgcgt tgccgtggac gtttatggtt
    52741 cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc cattattatg ggtcttgcag
    52801 gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg ttttattcgc gacgtagaag
    52861 agcgcgaacg tctgctgctg gcacagggac ggcatgaagt ggatttcccg cgcgacaacg
    52921 ggatgcgttt tcataacggc aacctgccgc tgcatgaaaa cggtatgcaa atccacgcct
    52981 ataacggcga tgaagtcatc tacagcaaaa cttattattc catcggcggc ggttttatcg
    53041 tcgatgaaga acactttggt caggatgctg ccaacgaagt gagcgtgccg tatccgttca
    53101 aatctgccac cgaactgctc gcgtactgta atgaaaccgg ctattcgctg tctggtctcg
    53161 ctatgcagaa cgaactggcg ctgcacagca agaaagagat cgacgagtat ttcgcgcatg
    53221 tctggcaaac catgcaggca tgtatcgatc gcgggatgaa caccgaaggt gtactgccag
    53281 gcccgctgcg cgtgccgcgt cgtgcgtctg ccctgcgccg gatgctggtt tccagcgata
    53341 aactgtctaa cgatccgatg aatgtcattg actgggtaaa catgtttgcg ctggcagtta
    53401 acgaagagaa cgccgccggt ggtcgcgtgg taactgcgcc aaccaacggt gcttgtggca
    53461 ttgttccggc agtgctggct tactatgacc actttattga atcggtcagc ccggacatct
    53521 atacccgtta ctttatggca gcgggcgcga ttggtgcatt gtataaaatg aacgcctcta
    53581 tttccggtgc ggaagttggt tgccagggcg aagtgggtgt tgcctgttca atggctgctg
    53641 cgggtcttgc agaactgctg ggcggtagcc cggaacaggt ttgcgtggcg gcggaaattg
    53701 gcatggaaca caaccttggt ttaacctgcg acccggttgc agggcaggtt caggtgccgt
    53761 gcattgagcg taatgccatt gcctctgtga aggcgattaa cgccgcacgg atggctctgc
    53821 gccgcaccag tgcaccacgc gtctcgctgg ataaggtcat cgaaacgatg tacgaaaccg
    53881 gtaaggacat gaacgccaaa taccgcgaaa cctcacgcgg tggtctggca atcaaagtcc
    53941 agtgtgacta atacttctta ctcgcccatc tgcaacggat gggcgaattt atccccggtt
    54001 tctcgtctgc tgtaatattc cccactacac ttccactgtt gcgtcaggcg tttgtcgcca
    54061 tacgcttaca gggtggcccg catgcaaaaa gcacaacgga tcattaaaac ctatcgccgt
    54121 aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc tcgcttcgac cctgagcgtg
    54181 cgatttattt cacagcgtaa cttaaatcaa caacgggtag tacaattcgc caatcacgct
    54241 gtagaggaat tagataaagt actgcttccc ctacaggcag gtagcgaagt cttgcttccg
    54301 ctgattggtc tgccctgctc tgtcgcccat ttgccattac gtaaacaggc ggcaaaactc
    54361 caaactgtgc gatccattgg cctggtgcaa gacggcacac tttattgctc cagcattttt
    54421 ggttatcgca atgtgcccgt cgtggacatt ctggctgaac ttcctgcacc gcaaccactt
    54481 ttacgcctga cgatcgaccg tgccctgatt aaaggcagtc cggttttgat tcaatggaca
    54541 cctgcagcgg gcagtagcaa tgctggggtc atggagatga ttaacatcga cttactgacg
    54601 gcaatgctcc ttgagccaca actgccgcaa atcagtagcg ccagcctgac ggtggacaaa
    54661 cggcatttgc tctatggtaa tgggctggta gattcccttc cgcaacctga agacaatgaa
    54721 aactatcagg tttcttcgca acgctttcct tttaccatta acgttaatgg tccgggggct
    54781 acggcgctgg catggcacta tcttccaaca caattaccgc tggcggtgct gctaagttta
    54841 ctggtgggct atatcgcctg gctggcgacc gcttaccgga tgagtttttc ccgcgaaatc
    54901 aatctgggcc tggcgcaaca tgagttcgaa ttgttctgcc agcctttgct taatgcgcgc
    54961 agccagcaat gtattggtgt agagattttg ctgcgctgga acaatccacg tcagggctgg
    55021 atttcaccgg atgtgtttat tcctatcgcg gaagaacatc atttaattgt gccactgacc
    55081 cgctatgtga tggcagaaac catccgtcag cggcatgttt tcccaatgag tagtcagttt
    55141 catgttggca ttaacgtcgc acccagtcat tttcgccgtg gtgtgctgat aaaagatctc
    55201 aatcagtact ggtttagcgc tcacccgatt cagcaactga tcctcgaaat caccgaacgc
    55261 gatgccttac tggatgttga ttatcggatt gcccgcgagc tgcatcgtaa aaacgtcaaa
    55321 ctggcgattg atgacttcgg cacaggcaat agttcatttt cctggcttga aacattacgt
    55381 cctgacgtgc tgaaaattga taagtcattt accgcagcta taggttctga cgcggttaac
    55441 tcgacggtga ccgatatcat catcgcgctg gggcaaagac tgaatattga actggtggcg
    55501 gagggcgtgg aaacgcaaga acaggcgaag tatttgcgcc gtcatggcgt gcatattttg
    55561 caagggtatt tgtacgcaca gccgatgccg ctacgtgatt ttcccaaatg gctggcgggc
    55621 agccaaccgc cgcccgcccg gcataatgga catatcacgc ccattatgcc gttacgttaa
    55681 cgcttactca tcttcatcgt gcgccggttg ctctttaaca atgcgaacca gatcaacacg
    55741 ataatcattg gcttcaatga tggtgatatg cagtggccct acatcaatca catcgcccac
    55801 acggggaatg tgaccatttg ccgagatcac gaggcccgcg accgtcgcga tatcatcgtc
    55861 atcggcaagg tgctcaacat caagcgcctg ctgcaaggca tgcaaatctg taccgccttt
    55921 taccagccag ccgtcaccat cggtaatgat ttccggcgtt tcgtctgcgt ccgggaattc
    55981 acccgcaatg gcttccagca catccagcgg cgtgaccaga ccttgtacca caccaaactc
    56041 gttggtcacg ataacaaagc tcccgcgagc acgacgcagc acgcccagca ggttgatcgg
    56101 gtcaagggtt tccggaacga taattgccgg agacgccgaa gctatcgccg ccacatcaac
    56161 gccctcttcc agcgccacca gcagttcttt agcacgtacg ataccgatga tttcatccag
    56221 ttcaccgcga cataccggga acagactgtg cggtgaggag agcagctgct cgcggatttc
    56281 atcgaccccg agattagcgt caacccagct tatttcaccg cgcggcgtca tgatcccgcg
    56341 cagagaacgc gacgccagcg tcaatacgcc gttaatcatg taacgttctt cttcggcaaa
    56401 cgcaccttcc gggatcggca tcggcatcgg gttatcggca tcgtgctgaa cattggcctg
    56461 acgtttcccg cccatcaaac gcaggatggc atcggcagta cgcgcacgca gcggcaaagt
    56521 cgactggtga cgaataaagt tgcgacgcgc aatctggtta aacacttcga tgatgatcga
    56581 gaagccaatc gcggcataca ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac
    56641 cagactcaga cctatcatta acaggaagct cagacagagc accaccaccg ttggatgctg
    56701 gttaacaaat cgcgtcagcg gtttcgatgc cagcagcata accgccatcg caatcactac
    56761 cgccgccatc atcaccggca gatggttaac catccctact gccgtaatta ccgcatccaa
    56821 cgagaagacg gcgtcaagga tgacgatctg tgtgacgacc acccagaaac tggcgtagcc
    56881 tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt tcatgcagtt cggttgttgc
    56941 tttgaacagc aagaatatcc ccccgaacaa cataatcagg tcgcgtccgg agaaggaaaa
    57001 atccataacg gtaaatagcg gtttggtcag cgtgaccatc catgaaatca gcgacagcag
    57061 ccccagacgc ataatcagcg ccagtgataa tcccagcaaa cgcgctttat cgcgttgttt
    57121 tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg ttatcgatac ccagcacaat
    57181 ttcgagaaca acaagcgtga gtagccccgc ccaaattgag gggtccatta agaattccat
    57241 gacaagctcc tgcttaagga atagctattc gacgccagaa ataatgcagg cgtaacgaca
    57301 aaatgcaaac gaaaggtgcg gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg
    57361 ctgaaatgac gtcggtgacg atccatactg cgggctactg ccctatactc catggttgtt
    57421 aaacgggagt taaacatatc agagacgcct ctgatttggc aaagatttac cttcctttgc
    57481 aaacgaatgt gacaaggata ttttaccttt cgaaatttct gctaatcgaa agttaaatta
    57541 cggatcttca tcacataaaa taattttttc gatatctaaa ataaatcgcg aaacgcaggg
    57601 gtttttggtt gtagccctta tctgaatcga ttcgattgtg gacgacgatt caaaaataca
    57661 tctggcacgt tgaggtgtta acgataataa aggaggtagc aagtgaccat tgctattgtt
    57721 ataggcacac atggttgggc tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc
    57781 gagcaggaaa acgtcggctg gatcgatttc gttccaggtg aaaatgccga aacgctgatt
    57841 gaaaagtaca acgctcagtt ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt
    57901 gatacatggg gaggcagccc gttcaatgct gccagccgca ttgtcgtcga caaagagcat
    57961 tatgaagtca ttgcaggcgt taacattcca atgctcgtgg aaacgttaat ggcccgtgat
    58021 gatgacccaa gctttgatga actggtggcg ctggcagtag aaacaggccg tgaaggcgtg
    58081 aaagcactga aagccaaacc ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca
    58141 aaagcggctc caactccggc aaaaccaatg ggaccaaacg actacatggt tattggcctt
    58201 gcgcgtatcg acgaccgtct gattcacggt caggtcgcca cccgctggac caaagaaacc
    58261 aatgtctccc gtattattgt tgttagtgat gaagtggctg cggataccgt tcgtaagaca
    58321 ctgctcaccc aggttgcacc tccgggcgta acagcacacg tagttgatgt tgccaaaatg
    58381 attcgcgtct acaacaaccc gaaatatgct ggcgaacgcg taatgctgtt atttaccaac
    58441 ccaacagatg tagagcgtct cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt
    58501 ggtatggcat tccgtcaggg taaaacccag gtgaataacg cggtttcggt tgatgaaaaa
    58561 gatatcgagg cgttcaagaa actgaatgcg cgcggtattg agctggaagt ccgtaaggtt
    58621 tccaccgatc cgaaactgaa aatgatggat ctgatcagca aaatcgataa gtaacgtatt
    58681 gtgttgatta tcactcagtt ttcacactta agtcttacgt aaacaggaga agtacaatgg
    58741 agattaccac tcttcaaatt gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat
    58801 caatcctcga tgaatttcag tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg
    58861 ttcttggcga tatgaaaacc ggtattatta tcggtggtac gctggaaatg atcgcgctgg
    58921 gctggatgaa catcggtgct gcagttgcgc ctgacgccgc tctggcttcc atcatttcta
    58981 ctattctggt tatcgcaggt catcagagca ttggtgcagg tattgcactg gcaatccctc
    59041 tggccgctgc gggccaggta ctgaccatca tcgttcgtac tattaccgtt gctttccagc
    59101 acgctgcgga taaggctgct gataacggca acctgacagc gatttcctgg atccacgttt
    59161 cttctctgtt cctgcaagca atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg
    59221 ttggtaccag cgaagtacag aacatgctga acgcgattcc ggaagtggtg accaatggtc
    59281 tgaatatcgc cggtggcatg atcgtggtgg ttggctatgc gatggttatc aacatgatgc
    59341 gtgctggcta cctgatgccg ttcttctacc tcggcttcgt aaccgcagca ttcaccaact
    59401 ttaacctggt tgctctgggt gtgattggta ctgttatggc agtgctctac atccaactta
    59461 gcccgaaata caaccgcgta gccggtgcgc ctgctcaggc agctggtaac aacgatctcg
    59521 ataacgaact ggactaacag gtgagcgaaa tggttgatac aactcaaact accaccgaga
    59581 aaaaactcac tcaaagtgat attcgtggcg tcttcctgcg ttctaacctc ttccagggtt
    59641 catggaactt cgaacgtatg caggcactgg gtttctgctt ctctatggta ccggcaattc
    59701 gtcgcctcta ccctgagaac aacgaagctc gtaaacaagc tattcgccgt cacctggagt
    59761 tctttaacac ccagccgttc gtggctgcgc cgattctcgg cgtaaccctg gcgctggaag
    59821 aacagcgtgc taatggcgca gagatcgatg acggtgctat caacggtatc aaagtcggtt
    59881 tgatggggcc gctggctggt gtaggcgatc cgatcttctg gggaaccgta cgtccggtat
    59941 ttgcagcgct gggtgccggt atcgcaatga gcggcagcct gttaggtccg ctgctgttct
    60001 tcatcttgtt taacctggtg cgtctggcaa cccgttacta cggcgtagcg tatggttact
    60061 ccaaaggtat cgatatcgtt aaagatatgg gtggtggttt cctgcaaaaa ctgacggaag
    60121 gggcgtctat cctcggcctg tttgtcatgg gggcattagt taacaagtgg acgcatgtca
    60181 acatcccgct ggttgtctct cgcattactg accagacggg caaagagcac gttaccactg
    60241 tccagactat tctggaccag ttaatgccag gcctggtacc actgctgctg acctttgctt
    60301 gtatgtggct actgcgcaaa aaagttaacc cgctgtggat catcgttggc ttcttcgtca
    60361 tcggtatcgc tggttacgct tgcggcctgc tgggactgta agactgttgt acactaccgg
    60421 ggccttttgg ccccgttttt tttatctgga ggattaatga caatcacgga cctggtactg
    60481 attcttttca tcgccgcact cctggccttc gcgatctacg atcagttcat catgccccgc
    60541 cgtaacggcc ccactctgct ggcaattcct ttgctccggc gtggtcgcat cgatagcgtt
    60601 atcttcgtcg gattgattgt cattcttatc tataacaacg tcacgaatca tggcgcgcta
    60661 ataacaacat ggttattaag tgcactggct ctgatgggtt tttatatatt ctggatccgc
    60721 gttccgaaga tcatctttaa acaaaaaggt tttttcttcg ccaatgtctg gattgaatat
    60781 agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt tggtgatgca attagaacag
    60841 cgtcggctgt taatccgcgt tcgaaatatc gacgatctgg aaaaaattta taagcttctc
    60901 gtttcaactc aataagttat gaatttagcc aaagctatg
//
LOCUS       DARNVM010000010        55192 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.010, whole genome shotgun sequence.
ACCESSION   DARNVM010000010 DARNVM010000000
VERSION     DARNVM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.010"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(121..1593)
                     /gene="der"
                     /locus_tag="SI621_000601"
     CDS             complement(121..1593)
                     /gene="der"
                     /locus_tag="SI621_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEI4063355.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(1711..2889)
                     /gene="bamB"
                     /locus_tag="SI621_000602"
     CDS             complement(1711..2889)
                     /gene="bamB"
                     /locus_tag="SI621_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEI4063356.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(2900..3520)
                     /gene="yfgM"
                     /locus_tag="SI621_000603"
     CDS             complement(2900..3520)
                     /gene="yfgM"
                     /locus_tag="SI621_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HEI4063357.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3538..4812)
                     /gene="hisS"
                     /locus_tag="SI621_000604"
     CDS             complement(3538..4812)
                     /gene="hisS"
                     /locus_tag="SI621_000604"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEI4063358.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNCTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(4923..6041)
                     /gene="ispG"
                     /locus_tag="SI621_000605"
                     /gene_synonym="gcpE"
     CDS             complement(4923..6041)
                     /gene="ispG"
                     /locus_tag="SI621_000605"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEI4063359.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFEQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6068..7081)
                     /gene="rodZ"
                     /locus_tag="SI621_000606"
     CDS             complement(6068..7081)
                     /gene="rodZ"
                     /locus_tag="SI621_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HEI4063360.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(7366..8520)
                     /gene="trmG/rlmN"
                     /locus_tag="SI621_000607"
     CDS             complement(7366..8520)
                     /gene="trmG/rlmN"
                     /locus_tag="SI621_000607"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HEI4063361.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8670..9101)
                     /gene="ndk"
                     /locus_tag="SI621_000608"
     CDS             complement(8670..9101)
                     /gene="ndk"
                     /locus_tag="SI621_000608"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEI4063362.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9250..11562)
                     /gene="pbpC"
                     /locus_tag="SI621_000609"
     CDS             complement(9250..11562)
                     /gene="pbpC"
                     /locus_tag="SI621_000609"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HEI4063363.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPTNLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLMDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            complement(11563..16524)
                     /gene="yfhM"
                     /locus_tag="SI621_000610"
     CDS             complement(11563..16524)
                     /gene="yfhM"
                     /locus_tag="SI621_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HEI4063364.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSEKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIQVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSVSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGVSRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDVINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGQSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            16731..17576
                     /gene="sseA"
                     /locus_tag="SI621_000611"
     CDS             16731..17576
                     /gene="sseA"
                     /locus_tag="SI621_000611"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEI4063365.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNATFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGAFNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            complement(18076..18852)
                     /gene="sseB"
                     /locus_tag="SI621_000612"
     CDS             complement(18076..18852)
                     /gene="sseB"
                     /locus_tag="SI621_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HEI4063366.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(18971..20254)
                     /gene="pepB"
                     /locus_tag="SI621_000613"
     CDS             complement(18971..20254)
                     /gene="pepB"
                     /locus_tag="SI621_000613"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HEI4063367.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20499..20699)
                     /gene="iscX"
                     /locus_tag="SI621_000614"
     CDS             complement(20499..20699)
                     /gene="iscX"
                     /locus_tag="SI621_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEI4063368.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20711..21046)
                     /gene="fdx"
                     /locus_tag="SI621_000615"
     CDS             complement(20711..21046)
                     /gene="fdx"
                     /locus_tag="SI621_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEI4063369.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21048..22898)
                     /gene="hscA"
                     /locus_tag="SI621_000616"
     CDS             complement(21048..22898)
                     /gene="hscA"
                     /locus_tag="SI621_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEI4063370.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22915..23430)
                     /gene="hscB"
                     /locus_tag="SI621_000617"
     CDS             complement(22915..23430)
                     /gene="hscB"
                     /locus_tag="SI621_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HEI4063371.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23526..23849)
                     /gene="iscA"
                     /locus_tag="SI621_000618"
     CDS             complement(23526..23849)
                     /gene="iscA"
                     /locus_tag="SI621_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEI4063372.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23866..24252)
                     /gene="iscU"
                     /locus_tag="SI621_000619"
     CDS             complement(23866..24252)
                     /gene="iscU"
                     /locus_tag="SI621_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEI4063373.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24280..25494)
                     /gene="iscS"
                     /locus_tag="SI621_000620"
     CDS             complement(24280..25494)
                     /gene="iscS"
                     /locus_tag="SI621_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEI4063374.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25606..26094)
                     /gene="iscR"
                     /locus_tag="SI621_000621"
     CDS             complement(25606..26094)
                     /gene="iscR"
                     /locus_tag="SI621_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEI4063375.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26364..27104)
                     /gene="trmJ"
                     /locus_tag="SI621_000622"
     CDS             complement(26364..27104)
                     /gene="trmJ"
                     /locus_tag="SI621_000622"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HEI4063376.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27223..28026
                     /gene="suhB"
                     /locus_tag="SI621_000623"
     CDS             27223..28026
                     /gene="suhB"
                     /locus_tag="SI621_000623"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HEI4063377.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28171..29025
                     /locus_tag="SI621_000624"
     CDS             28171..29025
                     /locus_tag="SI621_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4063378.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAENAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESCSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29216..30496
                     /gene="csiE"
                     /locus_tag="SI621_000625"
     CDS             29216..30496
                     /gene="csiE"
                     /locus_tag="SI621_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HEI4063379.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALTGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30488..31627)
                     /locus_tag="SI621_000626"
     CDS             complement(30488..31627)
                     /locus_tag="SI621_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HEI4063380.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(31787..32677)
                     /gene="hcaR"
                     /locus_tag="SI621_000627"
     CDS             complement(31787..32677)
                     /gene="hcaR"
                     /locus_tag="SI621_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HEI4063381.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTV"
     gene            32813..34174
                     /locus_tag="SI621_000628"
     CDS             32813..34174
                     /locus_tag="SI621_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEI4063382.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDSIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            34171..34689
                     /locus_tag="SI621_000629"
     CDS             34171..34689
                     /locus_tag="SI621_000629"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEI4063383.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            34689..35009
                     /locus_tag="SI621_000630"
     CDS             34689..35009
                     /locus_tag="SI621_000630"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HEI4063384.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            35006..35818
                     /gene="hcaB"
                     /locus_tag="SI621_000631"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             35006..35818
                     /gene="hcaB"
                     /locus_tag="SI621_000631"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HEI4063385.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            35828..37030
                     /locus_tag="SI621_000632"
     CDS             35828..37030
                     /locus_tag="SI621_000632"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HEI4063386.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSLQLQQVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHADDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAILGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     RGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRTIRKWIQSGKTFNAKLLID
                     EDIALKSL"
     gene            37127..37549
                     /locus_tag="SI621_000633"
     CDS             37127..37549
                     /locus_tag="SI621_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEI4063387.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(37596..38468)
                     /locus_tag="SI621_000634"
     CDS             complement(37596..38468)
                     /locus_tag="SI621_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEI4063388.1"
                     /translation="MTIYTLSHGPLKLDVSDRGGVIEGFWRDATPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVNNQGTETLPFGTGLHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPCQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(38480..39574)
                     /locus_tag="SI621_000635"
     CDS             complement(38480..39574)
                     /locus_tag="SI621_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEI4063389.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLSQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(39607..40605)
                     /locus_tag="SI621_000636"
     CDS             complement(39607..40605)
                     /locus_tag="SI621_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063390.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(40630..42141)
                     /locus_tag="SI621_000637"
     CDS             complement(40630..42141)
                     /locus_tag="SI621_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEI4063391.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(42164..43147)
                     /locus_tag="SI621_000638"
     CDS             complement(42164..43147)
                     /locus_tag="SI621_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEI4063392.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGAKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(43244..46525)
                     /locus_tag="SI621_000639"
     CDS             complement(43244..46525)
                     /locus_tag="SI621_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEI4063393.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGTVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDQTEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHNEKCLMVAMLASAGLGEVAHYESARAELTAINPAWPKAALFTTVMPFI
                     FNSVH"
     gene            46643..47830
                     /locus_tag="SI621_000640"
     CDS             46643..47830
                     /locus_tag="SI621_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEI4063394.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLR"
     gene            complement(47900..49153)
                     /gene="glyA"
                     /locus_tag="SI621_000641"
     CDS             complement(47900..49153)
                     /gene="glyA"
                     /locus_tag="SI621_000641"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEI4063395.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            49510..50700
                     /gene="hmpA"
                     /locus_tag="SI621_000642"
     CDS             49510..50700
                     /gene="hmpA"
                     /locus_tag="SI621_000642"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HEI4063396.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKVLGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(50745..51083)
                     /gene="glnB"
                     /locus_tag="SI621_000643"
     CDS             complement(50745..51083)
                     /gene="glnB"
                     /locus_tag="SI621_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HEI4063397.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(51144..52478)
                     /gene="glrR"
                     /locus_tag="SI621_000644"
     CDS             complement(51144..52478)
                     /gene="glrR"
                     /locus_tag="SI621_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HEI4063398.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(52468..53181)
                     /gene="qseG"
                     /locus_tag="SI621_000645"
     CDS             complement(52468..53181)
                     /gene="qseG"
                     /locus_tag="SI621_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HEI4063399.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAINTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYNDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(53346..54773)
                     /gene="qseE"
                     /locus_tag="SI621_000646"
     CDS             complement(53346..54773)
                     /gene="qseE"
                     /locus_tag="SI621_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HEI4063400.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLVDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADGAVELENVELAPLVEMV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT        13021 a        15316 c        13780 g        13075 t
ORIGIN      
        1 gccgttatcc tgatcaagca catgctgaca ttgtggacaa tgcagttcca tatgaatttc
       61 tccggtagcc attccctcta cattcataga gggaatggca gataaaatac ttacggataa
      121 ttatttattt ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt
      181 cagggtgtta cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg
      241 cgatcccatt acgtccagcg atttgcggaa gtagttcatc aagtaacgct tgtaggaatc
      301 aggcaggtct ttcacctgat taccgtgaat caccacaatc ggcgggttat aaccaccggc
      361 gtgggcatat ttcagcttca cacgacgacc gcgtaccaac ggcggttggt gatcttcaac
      421 agccatcgtc atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata
      481 cgcttcacgt actgattcaa acagattacc aacaccactg ccgtgcaagg ctgagataaa
      541 atgcacacga gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc
      601 tttcacttcc tggctcaggc catcccactt attcaccaca atgacaagtg agcgcccact
      661 attgagaata aagcccagca gggagagatc ctgatcggaa ataccttcgc gcgcatcaat
      721 cactaacatc accacgttgg catcttcaat ggcctgcaac gttttgatta ccgagaattt
      781 ctctacagca tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata
      841 ctcacgtcca tcgcgttcca ttggaatgta gatactgtca cgcgtcgtac caggcatatc
      901 ataaacaaca acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt
      961 cggacgaccc acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc
     1021 ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag tattcggcgt cttcgtcgac
     1081 ttcttcttgc ggtgcgagat cttccatcca cggcagcagc acgtgctcca gcagacttaa
     1141 tacgccacga ccgtgagacg cggcgatcgg gtagatttcc cctaaaccaa gcgagtagaa
     1201 atcaaccacc gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg
     1261 tttttcacgg gagcgcagat gtttggcaat cgcttcatcc gccggcatca ggcccgcgcg
     1321 cgcatccacc ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc
     1381 catacgtgtc tctacaccgt cttcggtgcc atcaatccca ccagtatcaa tacaaataaa
     1441 ctcgcggcct tcaatttctg cacgaccgta cttacggtca cgagtcagac ccgggaaatc
     1501 cgcaaccagc gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt
     1561 agggcgcccg acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat
     1621 cagacaacgc acgctatatt cgtgtcgttg ttaaaaacag gaaaacggcc cctgtccagg
     1681 agccgttttc aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc
     1741 ttttgcctgg atcagcagtt tgccatcagc ggcaaccggt tcagtctgga aaccggaact
     1801 atcaactttt tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcagataacc
     1861 ttcgctgtca cctaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg
     1921 caacagatcg ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg
     1981 gtcattttga tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc
     2041 cagttcgcgt ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt
     2101 ataggccagc gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag
     2161 acggtcaatt tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc
     2221 ctgttccatc agcactgcgc tgacgcgacc attatcgcca cccacgacgg ccgcaccaaa
     2281 agccgttgcc ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt
     2341 ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat
     2401 taacaccaga ccgtcgctga ccaccggacg cgaaagtgct tcacccgcga ctttagtttg
     2461 ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat
     2521 gtagacatgc ccaccagaca cggtcacacc gccagaaagt aatgcaggct ctttagagaa
     2581 ccagccatct ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag
     2641 cgcttttact aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg
     2701 aagattggaa tagaagttgc caatgccgct accaacggaa gtgctccacg ccgtggtcgg
     2761 cgtaaactgg ttttcaacgg tcggcaatgg ggacatcttt accacatctt cttcgctgtt
     2821 aaacagcgaa cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg
     2881 caattgcatc gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc
     2941 tcagtgccgg ggtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct
     3001 tatcaccttt gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt
     3061 cacctttgat ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgaacctgaa
     3121 cgcgagcaag acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc
     3181 cctgttgtaa ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa
     3241 gttccaaaga agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg
     3301 ccggaatgct atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag
     3361 aagcggagcg tgcagaatca acctgatggc tgttccagta gcgccagcca attagtgcgc
     3421 caacgcccaa aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa
     3481 ccgcttctac ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta
     3541 acccagtaac gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc
     3601 agagcgcaaa tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac
     3661 agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc
     3721 gtggttggtc atcaatttca cgcccggtaa ttcatcacgc agacgctctg ctaatgccat
     3781 agccgcagat tgtgtatcag cacctgaagc caccaggtat atatcgacaa caggatcggc
     3841 cttaaattcc ggattaacgg cctgaactaa caatacaaga cgttcgaggc ccatcgcaaa
     3901 accgacagcc ggtgtagcac gaccacccag ttgttccacc agaccgtcat aacgaccgcc
     3961 tgcacacacg gtgccctggg aaccgaggct gttagtcacc cactcgaaaa cggtacagtt
     4021 atagtaatcc aggccacgca ccagacgctg gttgacggtg taagcgatcc ccgcgctctc
     4081 aagcagtttg cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcgcctaa
     4141 tgccggagcg tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag
     4201 cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag
     4261 gaatgccacc agcgcatcgc ggtaattcgc acgagcttcc agcgaaccga tagagttcag
     4321 ctcaagtgtt acgtgctcgg aaatacccag cgcgcgccac cagcgggcag tgagcataat
     4381 cagttcagcg tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa
     4441 ctgacgataa cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag
     4501 acgctgttcc tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt
     4561 cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt
     4621 ttcaaccacg tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat
     4681 cggcaagcgg atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat
     4741 gcgctgccag atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat
     4801 gttttttgcc acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca
     4861 atgtttcggg agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt
     4921 tattattttt caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc
     4981 gcacgaatgc gcgcttccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc
     5041 acgccatctt catagaggcc gcttttcttg ttaccgccgg tgacgccgag tgtagaaacc
     5101 agcgcctcac ctgggccatt caccacacag ccgataatcg aaacgtccat cggagtgatg
     5161 atatcttcca ggcgttgctc cagcgcgtta accgtaccga taacatcaaa ctcctgacgc
     5221 gaacaggttg gacaggcgat gaagttgatc ccgcgcgaac ggatacgcag cgatttcaaa
     5281 atatcgaaac cgactttgat ctcttcaacc ggatcggccg ccagcgatac gcgcagcgtg
     5341 tcgccaatgc cttcagacag cagcagacct aaaccaatgg cggatttaac cgccccgctg
     5401 cgcgcaccac ccgcttcggt gatccccaga tgcagcggct gatcgatctg ttttgccagc
     5461 aaacgataag actcaacagc gaggaagacg tctgacgctt tcacgctgac tttgaactgt
     5521 tcgaagttca agcgatcgag atgatcaaca tgacgcatgg cggattccag caacgcctgc
     5581 ggcgtcggtt cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca
     5641 atacggatcg gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca
     5701 ttaccgatat tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc
     5761 gcaatgcgat agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg
     5821 agtttgaacg cttctgccgc gtccatcgtc ggtactgaga cacggacgat atcagcgcca
     5881 acacgttcca acgccttgat ttgattgacc gttgcttcga cgtctgtcgt acgcgtattg
     5941 gtcatggact gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata
     6001 cgtgttgatt ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac
     6061 ccgtctgtta ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag
     6121 ttctgataaa acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg
     6181 gcgcaccaat tttcagttta tacggtgcct ggccggttaa attcaaatta ccgtctttac
     6241 gctgcatccc gctaaacagt tttttaccgg tagcatcagt gacctccagc cagcaatcgg
     6301 cagtaaagtt catcaccagc gcattcggat cagccgccgg cgtggtcacg ccagcctgat
     6361 cggttggcaa cggcgcagca ccatctggcg ttgttgttgc cgttggtgcc ggggtcgcgg
     6421 cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg
     6481 gtgctggcgc agttacggca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg
     6541 gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc
     6601 cctgctcgct attactgctc aattccgccg aagattgatc ggccatagtg gtgatctctt
     6661 cctgctgagc tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca
     6721 acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt tttgcggcgt ttaccgaggg
     6781 aaaaactctg catcggcgca acttttgcag cccgaagtgg agcctgcttt tccagccctg
     6841 gcagcagttc ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca
     6901 ggaatgttga agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa
     6961 ccttcaggca aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat
     7021 tacgcaggcg agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca
     7081 ttcgctgcag gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac
     7141 accgcgaaac atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg
     7201 tgacaaagcc cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa
     7261 gtcagcaaaa acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata
     7321 aaagattacg ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat
     7381 atcgatggct tcaccctgca tccgtttacg cagggtacgt ttcgtacggt cgataacatc
     7441 gcccgccaac tgaccacagg cagcatcgat atcatcacca cgagttttac gcacaatagt
     7501 ggtgaaaccg tagctcatca gcacttttga gaaacggtcg atacggctgt tcgaactgcg
     7561 tccatacggc gcgccaggga acgggttcca cgggatcagg ttgatcttac acggcgtatc
     7621 tttcagtagc tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat
     7681 cacgtattca atagtgactc ggccctgatt ggcgttggat ttctccagat aacggcgtac
     7741 cgcagcaagg aacgtttcga tattgtactt tttgttgatc ggcacaattt cgtcacgaat
     7801 ttcgtcgttc ggcgcgtgca gggaaattgc cagtgcaacg tcgatcatat cgcccagttt
     7861 atccagcgcc ggaactacac cggaagtgga aagcgtcacg cgacgtttag acaggccaaa
     7921 accgaaatca tcaagcatga tttccatcgc cggaacgaca ttgttcaggt tgagcagcgg
     7981 ctcgcccatg cccatcatca ctacgttagt gattggacgc tgaccggtga cttttgctgc
     8041 gccgacgatt ttcgctgcac gccacacctg gccgataatt tccgataccc gcaggttgcg
     8101 gttaaagccc tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga
     8161 agagacgcag agcgtggcac ggtcgtcttc cgggatatac accgtttcga cgcgctgatc
     8221 gccaacggca attgcccatt taatggtgcc gtcagatgaa cgctgttctt caaccacttc
     8281 cggtgcgcgg atctccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt
     8341 catctcatca aagttgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg
     8401 gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc tgctgacggt tgagatccag
     8461 caggttgatt tttccatctt tcgtggtgac gttttcaggt gtgactaatt gttcagacat
     8521 atgctattcc ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc
     8581 cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc
     8641 cacgtttgca cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg
     8701 ccgaagaaat aagcgatttc gcgagcggca gattcgacgg aatcagaacc gtgggtacca
     8761 ttttcggtca ggctgtcagc gtaatcagca cgcagagtac cggccagtgc gtttgccgga
     8821 ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt tttcaccctc cagcacggaa
     8881 accacgatcg ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg
     8941 tgttcagcat aaaagccacg agcctgttca acggtcaggt gcagcatttt ggtgccgaca
     9001 attttgaacc ctgcagcttc aaagcgcgca aagatattac ctatgacgtt ttttgctacc
     9061 gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca tttttacctc tgtaaattgt
     9121 tctgttgttg tctgtaccag cgtacgagat ggcgcggatt ataatgagca acagggccgt
     9181 tgactattga tgaaggtaac atttttttaa aataaaccta gtttaagcaa caatcaggga
     9241 caaaacagac tattgcatga caaatttcac tgtcgcgatt tgccccacct catccatcac
     9301 cagcaattga taatcgccct tatccatcaa atgcagggtc acgttgcgcc cgcgttcagt
     9361 taacggttcg ccattcagaa accaccagcg ttcacctgcc cctccacttg attgcaacgg
     9421 caaagtcgct tccgctgcgc ccggtaaacg tttaataatc gcgccatcgc gaacacctgt
     9481 cagctgcagt gggagttgtg catcgtgacc gtatggcggg caaattgtcg aggccggtgg
     9541 taagcgcaca gcgcggcgtt ctgatgcggg caaccagggt tccagcggta gcggccagac
     9601 atttatcatt tcttgtcgcg cctgagggca atcagcggca acacgtttgc cgttttcatc
     9661 cagccagatg gggaaacgaa tgccattaat gccttcctgc tccggcagta atagagttgg
     9721 tggctgactc ccgtccagca gccaggttgc caggcggcgg cggcagttac cgtcaccttc
     9781 cggcaaagac tgtccgcccg gccagcagat aacgccacga cttacagagt caggacgtgg
     9841 gtcttccggc agattcgcac tgcgcgacag taagatatta ttaacctgat tcaacaatgg
     9901 tacggcgctg gcaaagccaa actgaccaac aacgggcgtg ccgtccggtc tgccagtcca
     9961 gatcccaatg acatagcgag cgttaacccc aatcgcccag gcatcacgat agccatagct
    10021 ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag gcaccatccg gcaagggttg
    10081 cgcttcatca gccataatcc ggcgaatgat ccacgcagcc cccgacgaca ttaaaggccg
    10141 ttcaagcagc ggatcgtcag gctgtaagcg caatttacct gccttgccgt ggcgagcaaa
    10201 cgcggtatac gctgccgcca tatcttccag ttttgcacca gcgccgccga gaatgagcga
    10261 aagattcggc gcagcgccgt tgggcagata caacggcaat ccaacattgc gtaattttgc
    10321 cgcaaaccgt ttcggtccat aggcttccag cacctgcaca gcaggtaaat tcagcgagcg
    10381 caccagcgcc tcgctcatgc tgatcgggcc atgaaaaccg ctatcaaagt tacctggtcg
    10441 ataatcaccg gtgcgccggg gaacgtcttg cagcagtgat gccgggtgga tcaagccttc
    10501 atccagcgcc agaccataaa caaacggttt gagcaccgat cctggcgatc ggatcgcatt
    10561 gaccatatca acatgaccaa agcgtgaatc atcgttgaga tcaacagaac ccacccagcc
    10621 gcgaacacgc atatcggtat gatcaaccac gatcatcgcc agtgagctgc gcggtggtaa
    10681 tcgtcctttc cagttttgcg ccagttcttc cagacgtcgt tgaagaccgg catccagcgt
    10741 agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg cgcgaaaaca gcggtgccag
    10801 ttgcggcatt tgtcggggtg ccagccagat gggttcttcc cttgattctt ttacctgctc
    10861 acgggaccac acaccttgcg cagccatccg ttcgagcact ttattacgcg cggcttcggc
    10921 acgttccggc cagcgatccg ggcgaagacg gctgggagct tgcggcaaaa ccgccagcat
    10981 tgccgcctcg gaatagctta aattcgtagg cgattttccg agataagccc aacttgccgc
    11041 accgatcccc tgcaacgtac cgccaaacgg ggcacggtta agatacaagg tcagaatttc
    11101 acgcttagac agatgccact ccagttgcaa cgcgcgccag agctggcgaa ttttgccgcc
    11161 aaatgttttg gggtgaggat caagcagacg agcaacctgc atagtgagcg tgctgccacc
    11221 ggaaataacc cgtcccgaag tgagatcttg ccatgctgcg cgcgccaccg agaatggatt
    11281 cacccccgga tgcttccaga accagcgatc ttcataattg atcagcgctt caaggtaacg
    11341 tggagaaaca tcttcgattg ttaccggata acgccagatg ccttccgcat cggcaaagcg
    11401 ccagagcggc gtcccatcct gcgccacaac cactcgtgcg ggattgactt catgcagcgg
    11461 tagaggccag agtttatctg ctccccacgc tgcaagaaaa aggagaaagg gcgcagctgc
    11521 cagcgttatc cagcagccgc gtttggttaa caggcgaggc atttacggtc tgacaatcag
    11581 cagatcttca gccgcgccag tcgcccgcca ttggggaaca tacattgatt ccaccatcgg
    11641 ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc agatacacca gcgttaccgg
    11701 ttggtattca tcaacggcaa ccgccgccac aaagcgatca tcacggaact caatgtgctt
    11761 aatgctcgcc tgctgcatct ggttcagtaa gttctgcact tcgccaccac tttgctccag
    11821 gctggcgcta ccgttcgcca gattctggtt ttccagttcc agacccgcag gcagcagatc
    11881 cacgactaac gcatccggca cgctgttact ggcttttacc tgcaaccaga ccagcaccag
    11941 atcgccgcta cgtaacgagt ccagcgattt actctgacca tcagtaccaa gaatatgacg
    12001 ctcgatttgc agcacattgt tcgccggtaa aggtgcggat tgcggataac cgctggcatc
    12061 cacacgcagc cataacggct gatcgccact gttggtcacc tgcaaggtgg caagttgatc
    12121 gctattcaga ttgctgttta gcgttttctc gcctgtcagc ggctcagctg agaaagaggt
    12181 ttgcgcctgc catttacccg gtaaatcctg aattgtacgg gcagccagga acaacgcgtt
    12241 actttcctgg gtcgatagcc agcgttcacc aaatgcctgc tgcgaaagcg tgttcagcaa
    12301 agtgttctgc tcatcgggta gcagtttatt ttcttccagc aaggagagca tcaacgcgtt
    12361 gtcgcgcagt ggactaccgt aatcacccag ccatatccgc tcatcactat tacgcggcgt
    12421 tttcagcgcc aacgcaattg cttcttcacc acgcgtcgca tcacccatgg ttttcagcgc
    12481 aacgccaagt tgcagcagcg gtaaaccaga agcggcatct gcacgatgct cccagatttc
    12541 acgcagcgca cccagtggtg ctttttgctg acgggccagc accagcgcag cgtaagactg
    12601 tacggcgaat ttactggctt tgagattatc cgcgtacggg atcgacatca tgcccggatc
    12661 ttgtaaatag cgcagcagac gctcatttcc ccggttaatg acgtctgtcg gcacgctgta
    12721 cccctgctca cctgcgcgga ccaggaaatc catcacgtaa gccgtcagcc agtactcttc
    12781 gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca cgttgcattt gcagcaaacg
    12841 ggaaacgccg atatcgaccg atgcacggcg tttctcatca ctgtcgcctt tgatgcccaa
    12901 cgcctgcagt tgggctgcgt tggtataaag tgacgggaac aagccgctgg cggtttgctc
    12961 aagacagccg tacggatacg cttttaactc tttgatataa cgtgcgatgt tcagcggtgg
    13021 tttgccgctc aacaacaatt gcccttccag tgtaacaggc gagaagtttt gcaatccatc
    13081 cgccggaatc gcccatgtct caccaggctg caacgccgta ccgtaattaa ccgtttgtgc
    13141 cgggaacgcc ggacggacgc cgattttcca ctgcttatgc tgatcggcaa cggtttcacc
    13201 cggtaacgct aacccgctaa tggtggcctg aatttctcca tcgccataac ccggcaatgc
    13261 tcgcaccggg ataaacagcg tagtacgcac acctggcgct aattcaacgg ctgcgggtga
    13321 aacgctgacc agttcaagca aaccactggc ggtcagggca acgttcagtt tttgcggttt
    13381 atcggtaaga ttagtgatat ccagcgtcag acgcgaggta tcgccactcg ccataaagcg
    13441 cggcatgttc agttcagcaa tcaccggtgc ggcaactatc actttgcttt cgttgctacc
    13501 gaagtcatct gccgtccagg cttgcgccat gacgcgtagt tcaccgttaa aatcgccaat
    13561 cggcagtgta accgagcctt cgccctgttc gttgagcgtt accggcagcg cctgctgcgc
    13621 gacaatattg acgtgattga ccggcggttt accaccacgt ttcagctcat caccatcgcc
    13681 accaaaacgc agagctgcca gacgcccctg accttcaatg acctgaccgt aaatatcgta
    13741 aatgtctgca ccatagcgtt tctgaccaaa gaacgcctgc cacggatcgg gggtaacgta
    13801 atcagtaata ttcagcacac cgctatcaac ggcagacacc agcacattca cctgttttgg
    13861 catctcacca tttttattgc tggctttaat tttcacggtt aatggctgat tggggcgcat
    13921 tttggtgggg gtttccagcg ccagatcgag acgacggttt tcatcgccaa gcggcagatg
    13981 caacacccca actgcgcgtt ttggcgtcgc agagcgagat ttatcgccag gacgtaccac
    14041 cagcgtactt aaatacagat catgacgatt ccaggtttta tcgaccggaa tcgtcagatc
    14101 cagcccttga gccggaacat caatctcttg ccaccacagc ggcccttcac tggactcgac
    14161 catcgcataa cctttacccg ccgttggcgc ggcgatatgc aacttaatgg tgtcgccagg
    14221 gcgataactg gctttatcca gtttcagcgt gacacggtcc ggacgcactg cgccgctccc
    14281 gtcgctgttg tcctgccagc tatagccagc ccagaaacgc acactactga ccgcttcatt
    14341 cggcgctttg acttccagac gataagcgcc ccactctacc gggaaactga ccttgccggt
    14401 ttcgtccgct ttcagatcca gagtttgttc attttcaatc agatcttttt gatcaaactg
    14461 tgattgccag ccttcatctt ctgaccagtt ccagtagtaa tcgcggcgtt cgcgaatcag
    14521 gcgcacctgc aagcccgaca cggctttttt cacgccttgc gcatcgctat aaacgatgtc
    14581 aaaagcggcg ttactgcctt catcaacaat gggctgtttc accgtgctgt cagtgcgata
    14641 atcgtaaacc gatttcgagg cgaactgcgg acggatcccc ggcaatgcat cggcaggcca
    14701 gatagcctgc tcagcgcggc gcgtcaccgg gcgaccgccc gattccaaca aactaccctg
    14761 gaaaataacc tgtaatgggg aatgcgtttc cttccactgg ctttctgtag aaacttcgcc
    14821 acgcccttta tcatccagcg tcaactgaac ttcatccagc gtgcgggaaa gattttcggc
    14881 agctatatcg ccgaattcga aaccaggtaa ggctgacaca gcttcacgca gtgggcgcag
    14941 gaaaagttgc ccttgcaaag tattaccatt agcaggcgca ccatacaggt agtaccccac
    15001 cacggaaaat ctcacttcat ctttcggcgt tagcggggtt ttctcaccag tcagattcag
    15061 cgccatgcgc tctggcataa aatcttcgac gtggaaatcc cacatccggt actggttatc
    15121 gcccgtgtta gcgcgaatat gccacatacc ggttgccgca ttgctatcga gtggccaggt
    15181 aaagtggtag aggccattct ccggctgact aacgacgctc ctgagtacct gcccgtcggg
    15241 tttaatcacg tctaacttga tgggttgatc gggcaatgct tttccatctg catcacgcaa
    15301 taaaccattg aggatgactg tttcaccagg gcgataaaga tcgcgcgggc caaacatgaa
    15361 aaactgtttg ctatagcctg gcgcgccagc aatgttaaat tctgctaagt ccagcgccgg
    15421 aagttttaaa tcgagtagcg ttgtctgacc gtctttacgc gccagcagta atgccgcgtt
    15481 tttatcattt tccagctgca catgcccctg agcatcactg gttgcctgag tcagagtctg
    15541 ccctttctca tttaataaag agacttgtat tccttgctgg gccgcgccgt tttccagact
    15601 ttgggtaaag atatccagac gattgtgata acggtgagct gaaacgccga tatcacttaa
    15661 cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca gcctgattca tcacagccag
    15721 atacacgcct gcctgctgaa gcggtttgat atcacccagc ggcagcaata atttttcacg
    15781 ggtgttacgc gcaggattga gatcaaaccg tccggtataa accagatccg ccatctgcag
    15841 cagtttttca gactgccagt tcgccagcga attgcggtat tcccattggc taatgaatgc
    15901 aggcagagat tcgggcttca cacggaagaa gttaacatca acattgttaa cgttgagcgc
    15961 cattaccggc agcccttcaa taactttacc aggtaacaac gaaccacggc tggcaaaacc
    16021 gacgctgggt tggatatcgc gggtagttat agttttttcg taatctttac tgaaggttgc
    16081 gttgttgagt gctttgacct ccttgccaat agtgacgatc aaatcacgtt tcggttcgag
    16141 gtggcgtaaa cgcaactctt taagattatc tgacagctcc caggcaccat ccactttgcc
    16201 gcttttctta tcgacgacat ggataacgcg tgagaaatcc tgatccgggt cgagaggaat
    16261 ggagaacgtc agcaccagcg tggctgcgcc atcaagttga acttcagaga gatccagcaa
    16321 tgtcagcgcc ttaccggcac tctgttgggc cagtttttgt cgctccggcg cggaaagttt
    16381 tgctgaactc acgttttctg aagaggccgc tttagtaact tcagaaggcg catctttttt
    16441 caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc agcgccagca ttagcatgca
    16501 ggcggctacg cgtaactttt tcatttttca tccctgccac aatggcccgt tagcaacgtc
    16561 gaataattat tatgcgtgag aatttacgtt atgtaattca gtatcacgcc catacaatcc
    16621 gaattttagt gagaattgtg gctcagacag ccaaatcagc ctcttttgct tgttgcattt
    16681 cactaaacat ccgacaattt agcctaaccc cggcaaaaat ggagatgcct atgtccacga
    16741 catggtttgt aggagccgac tggctcgccg aacatattga tgacccggaa attcagatta
    16801 tcgatgcccg catggcgtcg cctggacagg aagatcgtaa cgttgctcag gagtatctga
    16861 atggacatat tcccggcgca gtgttttttg atatcgaagc gctttctgat cacacttccc
    16921 cgcttccgca catgctgccg cgcccggaaa cgttcgccgt ggcgatgcgt gagttaggcg
    16981 ttaaccagga taagcacctg attgtctatg acgaaggtaa tcttttctca gccccacgag
    17041 catggtggat gctgcgcacc tttggtgtag agaaagtgtc gattctgggg ggtggacttg
    17101 caggctggca gcgcgatgat ctgctgttag aagaaggtgc agtagagctg ccggaaggag
    17161 agtttaacgc cacgtttaat cctgaagccg tggtgaaagt caccgatgtg ttattggcaa
    17221 gccatgaaaa tacggcgcaa attattgatg cccgcccggc tgcacgtttt aacgcagaag
    17281 ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc cggcgcgttt aacgttccgt
    17341 ggacagaact ggtacgtgaa ggcgaactaa aaacgaccga tgaactggat gcgatatttt
    17401 ttggtcgcgg cgtaagttat gacaaaccta ttatcgtcag ttgcggttct ggtgtaacgg
    17461 cagccgtggt tttgttggca ctcgcgacgc tggatgtgcc aaacgtgaaa ctgtacgacg
    17521 gcgcatggag tgaatggggc gcacgggcag atttaccggt tgagccattg aaataagctt
    17581 tttacaggca ataaaaaacc gccgaatttg gcggtttttt attgctggtc tggttcgcgg
    17641 cctttccagc tggttgactt gtgttgctgg agcaacgcag gcaccccaca gtgttgaaat
    17701 actctctttt atgcctggct atgtcaagat aataaacaga gtaattattc aaacatgaga
    17761 gtattattcg tgacggccct tttcgccgtc tcgcaaacgg gcgctggctt taggaaagga
    17821 tgttccatga ccgtaaatgc agacacccca cagtgcttgc tatcgcggca atatcgccag
    17881 tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat gaagatacgg tgcttttgta
    17941 tcgtacttat tgtttctggt gcgctgttaa ccgaggtaaa taataaccgg agtctctccg
    18001 gcaacaattt actggtggtt aacaaccttc agagcagcaa gtaagcccga atgccgccct
    18061 ttagggcggc atattttaga ttatccggtt ctgtttaaag tcacgcaaaa aaccacccca
    18121 gcgacgttca tagaatggca caatatgttc ggtaataaag tggctaattc ctttttcccc
    18181 ttttttcacc tgacaaatat cgatgggttc atcgccaggt aatgtatcgg tcgctacact
    18241 tcccgccgcc tgaataattt cttcgatatc accatcggct tcaatgccaa taagtaaatt
    18301 aggctgtgcc tcttcgttct ctttaattga acaaataaaa gcacgcttca ccggcttaat
    18361 agttttaaat aaggtggtga gtgaatcaat catttgtgct ggcggctctg cgacttccga
    18421 taatatcagc gattcaccgc cctccagtac ttcctggctg ctcagcggat ttccttcttc
    18481 accaatcaac aaactgattt cacgcggcat aaattcttta ccggttggca gtttggcatt
    18541 aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg cgaacgggca ttacgacaaa
    18601 tgcctgttcg tcttcaaccg cctgttgaag tgcttctaac gaggtaaaaa aaggaatgac
    18661 gctggtgccg tcttcttttt cccagtgctg taaatcaagc gcgctatctt caaccacagc
    18721 ctcgccctgc gccgccgtac ccggcaccca gacggtggat tccagtagag tacggaaaaa
    18781 ggccgggcgg tgcgccggtt cggttgctgc tttttccagc agatcttcaa gttcgttttt
    18841 tgtttcggac ataagaacca caattcattc aacgttcggg gcacaagtgc ctgatgcgct
    18901 acgcttatca ggcctacaag ggaatctcaa tctattggat tttcgcaatt ttgtaggcca
    18961 aataagacga ttacgccgtt aacagattag caatcgtgcg cacaccaagt cccgtagcac
    19021 ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc
    19081 agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg
    19141 ccgggtacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc
    19201 ggtggaactc cgccagcggc agacgccaga acggttcatt ctcctgcgcg gcactcgcca
    19261 gcaagcgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg
    19321 cagttttcgc cgccccggtg agggtcgccg catcaatgat cagttccggt ttctgcgcac
    19381 tggcatcaat caggccatcg gccagcacca gacgcccttc cgcatcagtg ttcatcactt
    19441 caactttttt accgttgcga taggtaataa tatcgcccag tttgaacgcg ttgccgctga
    19501 tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg
    19561 caaacgccag cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca
    19621 taaatgcggt ctgtttgata ctgtagccgc cggaatcaaa ggtgatgcct ttaccaacca
    19681 ggcacgcgta cactggcgct tctttatcgc cagtcgggtt gtaatccagc gccagcaata
    19741 ccggagaacg ttctgaaccg cgtccgaccg tgtgcagccc catataacct tgatcacgca
    19801 gatcttcgcc tttggtgatc cggtaagtca cacgatcgcc cgcgacattg ctgatcagat
    19861 caacagcacg ctgtgccagt tgcgatggcc ccagttcttc cgccggtgcg ttgatggtgt
    19921 cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctgccgttgg gcatcgtcca
    19981 gatccggcca ctccacttta cgtgtgcctt tcggggcttt gtaaccttgc cagaatgccc
    20041 agcagcgatc cgcatcccag tcttcaccgc ttaactgaac atgcttgatg cccagaccgt
    20101 caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg
    20161 taatgccatc attattaatg ctgtaagtcg ctttttctcc ccagcgcgcg tcggcaggtt
    20221 gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg
    20281 ccgccagttg gcagccccag gatttttccg ctaaatgatt cgcgttgcag gacaaaacgc
    20341 attgcgtttt gaacagcgct tgcgctggcc ccaaaggggt gaggccaaaa ggccgaatca
    20401 cgcggcaagt gagtgaagcc ccaggagcat agataactat gtgactgggg tgaacgagcg
    20461 cagccaacgc atctgcggcg tgaagcatga cgcggaaatt attcggcttc gtccagccag
    20521 actaacaaaa tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct
    20581 tccagatcgc aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg
    20641 ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat cggtccactt aagtcccata
    20701 ctaacctctg ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgaccacta
    20761 aatcttcgtc ggtaacgcgc gcctggcagc ttaaacggct ttccggctcc agtccccagg
    20821 ctttgtccag catgtcgtct tcctgctctg agctttccgg cagcgagtca aaaccttcgc
    20881 gaacgatgca gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcga
    20941 taccgttacg cagcgcaacg tcgagaatgg tttcaccact attcgcttcc agaacagcgc
    21001 catcagggca gagatcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc
    21061 acggaatggc ctttcagcgc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc
    21121 tgggtttgtt tgtctacgtt tttaatcgct tgttcgatag cgtcaacatc atcgccctgc
    21181 gccacttcac tcaggtgagc ggcagcatcg tcaatgacct gacgctctgc ggtgcttaac
    21241 agcgcggcat cagcagccag cgcgccgtgc agactttcca gcacacgcgc cgcttctact
    21301 ttttgttctg ccagcatacg ggcttttacg tcctgctcgg catagctcat tgagtctttg
    21361 atcatcgaag cgatttcgct atcggtcaga ccataagacg gtttgacctg aatagacgcc
    21421 tcaacgccgg tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg
    21481 aacgttacgc gaatatgcgc gccgccagcc ggtagcgccg ggataccacg cagtgcaaaa
    21541 cgcgccagcg agcggcagtc ctgcaccagt tcacgttcac cctgcatcac atggatagac
    21601 attgccgtct gaccatcttt aaaggtggtg aaatcctggg cgcgggccac cggaatcgtg
    21661 gtattacgcg ggatcacttt ctccaccagg ccgcccatgg tctcgaggcc cagcgacagc
    21721 gggatcacat ccagcaacag catttcgctg tccggcttgt tacccaccag aatatccgcc
    21781 tgaatcgccg cgccaatagc gacgacttta tccgggtcga tagaagtcag cggtggacga
    21841 ccgaaaaatt cgcccacccg ttcacgcacc agcggcacgc gggtagaacc gccaaccatc
    21901 accacttcca gcacttcatc agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc
    21961 agtaaggttc gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg
    22021 ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat cgctcagcgc gattttggct
    22081 gcaatggcgg catccagcag ttcacgctga acgcggttat cgctacgatc aggaatgccc
    22141 gcctgctcgc gaatataatc cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa
    22201 tcaccgccgg tcgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca
    22261 aacgtcccgc cgccgaggtc ataaacagcg atcacgcctt cctgaccgga atccagcccg
    22321 taggcgatag ccgcagcggt cggttcgtta agtaagcgca gaacatgcaa ccccgccaga
    22381 cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata
    22441 acgacaccat ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg
    22501 atgtccgcag aaacgcgcac cgggttcagc agccccgccg ccgtttcaat catcggcagg
    22561 ccgttttcgc tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc
    22621 agcgagcgcc ccatcaggcg tttaacagaa ctaatggtgt tggcggtatc gagcgcggcg
    22681 ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagacggc
    22741 agcaggtgac ggccttcatg gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc
    22801 accagcgagt tggttgtgcc caggtcgata ccggccgcca aacgacgctg atgcggcgca
    22861 gcactcaaac caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa
    22921 atcgagcagt ttttcttcga gttgttcggc actgcttcgc agtttatcga gaaaacgcag
    22981 cttacgcacg gtatccgccg ccgcgtccca cgtctcgttg tctaactgtt caaccatcaa
    23041 ctgatggcgg gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc
    23101 atctttcgcc tgttcgatct cgtccagttc ttcgcgcagc tccagctgtt ccatcaggaa
    23161 cgcggtatcg cgcacagtat gctgctcgct ggcgagatca aaaccgtgca aagaaagcaa
    23221 atattccgcg cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggttgcaga
    23281 ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg gcgaatttat caggatgata
    23341 ctgacgttgt agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt gataacgggc
    23401 aggcaagcca aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat
    23461 aacggcgaat caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat
    23521 gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat ctttgacgtt cgggttggtg
    23581 aatttaaacc cttcgttcag gccttctttt acgaagtcca gctgcgtacc gtccagaaat
    23641 tgcaggcttt tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc
    23701 ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac ctgaacatcc ggaggttctc
    23761 acgcccagac gcaggccaaa ccctttgccg cggttagcca gaaaggtatt tactcgcgct
    23821 gctgcactgt cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg
    23881 tttgctttta tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg
    23941 aattttcacc ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgcgc
    24001 ttcgtcgaga gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcaga
    24061 accgcagccg taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat
    24121 ctgcaacttc attacgtcgc cacaggccgg tgcacccacc atgccgctgc cgacgttctc
    24181 gtcgttgttg tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc
    24241 gctgtaagcc attataaatt ctcctgattc cgataccgat taatgatgtg cccattcgat
    24301 gctgttcaga tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg
    24361 accgatggat ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg
    24421 acctaaagag aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag
    24481 cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ccgcgaggtc
    24541 tttcagcgcc ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt
    24601 cggcgcgccg tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt
    24661 ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc
    24721 gatgcgatag gcttcgccca taccaacgat ctggtgaaca ggcagagtgc cggaacgcat
    24781 accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg
    24841 cacatacagc gcaccgatac ctttcgggcc atagattttg tggccggaga atgacatcag
    24901 gtcaactttc aactggctca ggtcgatagg cagtttgccc acgctctggg ttgcatcaac
    24961 gtgatagata atgccgcgag cacggcacat ttcgccgata gccgcgatat cctgcaccac
    25021 gccgatttcg ttatttacgt gcatgatgga aacgaggatg gtgtcgtcac gcatcgctgc
    25081 ttcaagttct ttcaggtcga taatgccatt acgctgcggt gccagatagg tgacttcgaa
    25141 accttcgcgc tccagctgac ggcaggtatc cagtaccgct ttgtgttcgg ttttgctggt
    25201 gatgatgtgc ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc
    25261 agattcggtt gcaccagagg taaagacgat ttcacgcggg tcagcgccga ccagatctgc
    25321 aatctgatta cgggcgatat caaccgcttc ttcagcctgc cagccgaaac ggtgagaacg
    25381 ggaggccggg ttaccaaagg ttccgtccat cgtcataaac tgcatcattt tctcggcaac
    25441 acgcgggtcc accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct
    25501 ataaactccg tacatcactc aatgcaagga accaggctac cggctggatg tacgaccgtg
    25561 tttacggagc gcgatacgcc ccggcctgat tctgaatact ttttattaag cgcgtaactt
    25621 aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga
    25681 cacatccagc acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc
    25741 ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg
    25801 gcagccgcct ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac
    25861 ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg
    25921 tacgctggaa accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag
    25981 ggaaattccc tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag
    26041 cgcaacgtca agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta
    26101 cttcacctca aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata
    26161 gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt
    26221 cgggtattta accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc
    26281 gacgtgaacg ccttatccga cctgaggctc tgtgcactgc aggcctgata agacgcgtta
    26341 agcgtcgcat cagacaacca ctattattcg gccttattac ctttattctg ctgctctata
    26401 gaagccagaa tcccgcgcag gatattcaat tcctggcttt ccgggcgcgc acgggtaaac
    26461 aaacgacgca atttattcat cacctgcccc ggatggtttt cacgaataaa accggttgcc
    26521 agcagcgttt gttccagatg accataaaaa cgctccagat catcgaccag cggatacggc
    26581 gtctcttcat gctcgacttg ttcgccattt tcctgagttg ccagccaggc catgcgcact
    26641 tcataggcaa taacctgaac cgccatcgcc agattcagcg aactgtattc cgggttagcg
    26701 gcaatcgcga catgataatg gcatttctgc aactcttcat tggtcaagcc gacgcgctcg
    26761 cgaccaaaaa ccagcgccac cggggtattt gccgcttcag cgacgctttt cagaccgcat
    26821 tcgcgcgggt cgagcatcgg ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc
    26881 aggctacaac cggctaacgc ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca
    26941 tcgctggccc ctgctgccag ggcaatcgcc tgggagtcag gtttcaccag tggattaacc
    27001 agccacagat tggttaatcc cattgttttc atggcacggg caacagaacc catattgccg
    27061 gtgtgtgacg tctccaccag cacaattcga atattttgca gcattgtctt tcttcgtcta
    27121 aagattattc gcgcatctta tcataaaacg aagacagatg ccgatctcgc tgctatactc
    27181 tgcgccgttt tcccgttctt taacatccag tgagagagac cgatgcatcc gatgctgaac
    27241 atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg ccaaaaacta tgaaaccccg
    27301 gacgctgtag aagcgagcca gaaaggcagt aacgacttcg tgaccaacgt agataaagct
    27361 gccgaagcgg tgattatcga cacgattcgt aaatcttacc cacagcacac catcatcacc
    27421 gaagaaagcg gtgaacttga aggtactgat caggatgttc aatgggttat cgatccactg
    27481 gatggcacta ccaactttat caaacgtctg ccgcacttcg cggtatctat cgccgttcgt
    27541 atcaaaggcc gcactgaagt tgctgtggtt tacgatccta tgcgtaacga actgttcacc
    27601 gccactcgcg gtcagggcgc acagctgaac ggctaccgtc tgcgcggcag caccgctcgc
    27661 gatctcgacg gtactattct ggcgaccggc ttcccgttca aagcaaaaca gtacgccacc
    27721 acctacatca acatcgtcgg caaactgttc aacgaatgtg cagacttccg tcgtaccggt
    27781 tctgcggcgc tggatctggc ttacgttgct gcgggtcgcg ttgacggttt ctttgaaatc
    27841 ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc
    27901 gtcagcgact tcaccggtgg tcataactac atgctgaccg gtaacatcgt tgctggtaac
    27961 ccgcgcgttg ttaaagccat gctggcgaac atgcgtgacg agttaagcga cgccctgaag
    28021 cgttaatgac tctggcgggt gatgtcactc acccgccctc gcctttcagg cgctattccg
    28081 aaatacttcc tcaccgcttt actttctttc gccactctcc caccatcttt ccccgattaa
    28141 tggatgataa agaagtcgca aggaaaggtt atggcgctgc cagtgaacaa acgcgttctc
    28201 aaaattctgt ttattctctt tgttgttgcc ttctgcattt atttaatacc gcgcatcgcc
    28261 atcaattttt tctattaccc tgacgacaaa atctacggcc ccgatccctg gtcagccgaa
    28321 tcagtagaat tcaccgctaa agatggcact cgcctacatg gttggtttat cccttctgca
    28381 actggccccg ctgaaaatgc cattgccacc attattcatg ctcacggcaa tgccggaaat
    28441 atgtccgccc actggccgct ggtcagttgg ttacccgagc gtaatttcaa cgtttttatg
    28501 tttgattatc gcgggtttgg taaatcaaaa ggcacgccgt cccaggccgg attgctggac
    28561 gatacgcaaa gtgccatcaa tgtggtgcgc catcgcagtg atgtaaaccc acaacggctg
    28621 gtgctgttcg ggcagagtat tggcggggcg aatattctgg atgttattgg tcagggtgat
    28681 cgtgaaggca tacgtgcggt gatcctcgac tccacatttg cctcttatgc gaccatcgcc
    28741 aaccagatga tccccggcag tggctactta cttgatgaga gttgcagcgg cgaaaattac
    28801 atcgccagcg tcagcccgat cccgctttta ctgattcacg gtaaagctga tcacgttatc
    28861 ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag agccaaaacg gttgatccta
    28921 atcccggatg gcgaacacat tgatgctttt tccgatcgtc acggcgatgt ttatcgcgaa
    28981 cagatggtgg attttatcct tagcgcgttg aatccgcaga actaacccat gatcgctagc
    29041 acgataatca ttcacaaaac caccttaaga catgctaatc cactggtcag aacagtttaa
    29101 gatgagaaaa attctgtgac gcttgccaac atttctgatg attagcattc ccttcgccat
    29161 ttccatgagc aaactttagc tattcttatc aattatgctt atgggagatc tacagatgat
    29221 gcctacgctt gctccaccat ctgtcctttc ggctccccag cgccgctgcc agatcttgct
    29281 gacactcttt cagccggggt tgaccgccac cacggcaact ttcagcgagc ttaatggtgt
    29341 ggatgatgat attgccagtc ttgatatcag cgaaacagga cgtgagatcc tgcgctatca
    29401 tcaactcaca ctgactactg gttatgacgg tagctaccgg gttgaaggta cagtgcttaa
    29461 ccaacgtttg tgtttatttc actggctacg acgtggtttc cgtctgtgtc cgtcatttat
    29521 taccagccat ttcacccccg ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa
    29581 cttttacgac gataccaatc tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa
    29641 acgctttgaa acacgcgata ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca
    29701 acaccacgct ggaattacgc ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg
    29761 ccttgaattt caggtagcgc aggaaattgg acgccactgg cagcgtcggg cgctccagcc
    29821 tgtaccgcct gatgagccac tgttcatggc attacttttt tccatgttgc gggttcccga
    29881 tccactgcgg gatgcgcatc agcgggacag acaactgcgt cagtctatca aacgtctggt
    29941 aaaccatttt cgtgagctgg gaaatgttcg tttttatgat gaacaggggt tgtgcgatca
    30001 gctttatacc cacctcgccc aggcgttaaa tcgcagtttg tttgccatcg gtattgataa
    30061 caccctgccg gaagagttcg cccgcctgta tccacgcctg gtgcgcacca cccgcgcggc
    30121 gctgaccgga tttgaaagtg aatacggcgt ccatctttct gatgaggaaa gcggtctggt
    30181 cgcggtgatt tttggtgcct ggctaatgca ggaaaacgac ctgcatgaga aacagattat
    30241 tctactgacc gggaatgata gtgagcgaga agcgcagatt gagcagcagc tacgcgaact
    30301 aacgttactg ccgctcaata ttaaacatat gtcggtaaag gtatttttgc agacaggcgc
    30361 tccgcgcagc gcggcactga ttattgctcc ttataccatg ccgttaccgc tcttttcacc
    30421 accgctgatc tatacggacc tgacgttgac aacacatcaa caggagcaga tccgcaaaat
    30481 gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa cattgccgga agcgccacca
    30541 gcgccataac ccagaacacg ccgtggccca gatattgata caggaaaccg gcgaaaacgg
    30601 tcatgatcgc aatactgccg cccatcgcga cggcagagta aaccgcctgt aaacggatga
    30661 cttcgctacc ctggcgagcg gcaatatagc gcatggcggc caggtggcag accgtgaagg
    30721 tgccacaatg cagaatttgc accactatca accacggcaa cgccgtagtt gctcccataa
    30781 tgccccagcg cactacgccg caaatcgctg agatcaacag catatcgcgt gcactacaac
    30841 ggcggaaaag tttattactc agcgcaaaga taatgacttc cgccaccacg cccagcgacc
    30901 acaaatagcc caccgccgag gccgagtagc cagcagcctg ccagtaaatg gcgctaaaac
    30961 cgtaataggc cgcatgcgcc ccctgcaata aacaaacgca ggccagaaag cgccagttct
    31021 ggcgaaccaa cgccaaccac gcagaccaac cggtgctctc ctgctggcgg cttgcccctt
    31081 gtggctgaat cgtcggacgg atgagaaagc cgagcagcat ggatgccacg cccaacgtca
    31141 acagcgcgag gatcacccga taatcaaaca tactgaccag tttgcccgtc agcgccgagc
    31201 caatgacaaa cgctaccgag ccccacagtc gcactttgcc gtaatcaagc gggaactgct
    31261 tttgccacgt attcgccagt gcatcggtca acggtaccag cggtgagaaa aagaggttaa
    31321 agccaatcat caccagcatc agccacgcta cgtgcgcccc cgcccagaat gcgacagcaa
    31381 agagaagtgt cagcagtgcc agcacgcgca aggcggaaat caggcgagaa ggatcgctga
    31441 cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac caaacctgcc cccaataaca
    31501 ggccgatggt ttctggcgtt aaaccaatcc ccttaagcca gacgctccag aaaggtagaa
    31561 aaatgccgta actaaaaaag tatgtgaaat agccgagcgc caaccagcgc gtggattgca
    31621 aaaccatgag tccctcccgt caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa
    31681 gttgaaaaat aagaatattc gagcctgctt cccgatgtct gtaacaccag ttcacaagca
    31741 ctaaaaaact gcgttccgcg acgcatgttg caaaaataat gcgtaattat accgttacgc
    31801 ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa tgccggcttc atctcccctt
    31861 tcttccacgc catcagtaaa gcaatggaag gcacatttcc ggcaatgggt cgaaatacca
    31921 cctggccggt attaaaatta ttcatataac cgggtatcaa agtgacgccc agccccatcc
    31981 ccaccagatt catggtcacc agaatattcg ttgccacctg gacgatattt ggctggctgt
    32041 tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga accggaatac gctggatcag
    32101 tactgacgaa attcacgcca tccagttgcg cggcagtgat ctctttttca tgtgctaaag
    32161 ggtgatcaac cggtaacacc accactaatg gttcgtcaaa aagctccaga taatcaatct
    32221 ccgggctata aacaggatgg cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt
    32281 cttcctgttc cgtggtgatt aaactcacca gctcaattaa ggtgtctggc tgtctgagac
    32341 gaaacatcgg taatactttt ggcagtaaat tcacttccgc cgatggcaca aagccaatgg
    32401 ttaattgtct gtcttcctga acaattttcc gcgcccgtaa tttggcattt tccgcttgtt
    32461 cgaggattgc cagcgcgtcc tggagaaaac attctcccgc cgccgtcaac gcgactttgc
    32521 gcttatccct caccagtaac ggaacaccga cacagttttc aagatcgcgg atctggctgc
    32581 ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg
    32641 ccactgcgac gaaatagcgt aaatgccgta gttccatcac cttccccttg ttatcgaaaa
    32701 aacgtctcag ctggtagaaa ttaactattt cacatattag caaccaacca gcaacatcct
    32761 tatggcacaa aaatagaagg tcaatacatc ttatctttca ggattaaaaa atatgaccac
    32821 accctcagat ttgaacattt accaactgat tgacacccaa aacggtcggg tcactccgcg
    32881 tatttatacc gacccggata tttaccaact ggagcttgag cgcattttcg gtcgttgctg
    32941 gctatttctc gcccacgaaa gccagatccc aaaacctggt gatttcttta acacctacat
    33001 gggggaagat gcggttgtcg tagtgcgtca gaaagacggc agcattaagg cgtttctcaa
    33061 ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt
    33121 tacctgtccg tatcacggct ggtcttatgg cattaacggc gagttgatcg atgtaccgct
    33181 ggaacctcgc gcctacccac aagggttgtg taaatcccac tggggactaa acgaagttcc
    33241 ttgtgtggag agttataaag ggctgatttt tggcaactgg gataccagcg caccaggcct
    33301 gcgtgattac ctgggtgaca ttgcctggta tctggatggc atgctggatc gtcgcgaagg
    33361 cggcaccgaa attgtcggcg gtgtacaaaa gtgggtgatc aactgcaact ggaaattccc
    33421 ggcagagcag ttcgccagtg accaatatca tgctctgttc agccatgctt ctgccgttca
    33481 ggtattaggg gcgaaagatg atggcagcga taagcgcctc ggtgatggac aaaccgcccg
    33541 tccggtatgg gaaaccgcca aagatgcgct gcaatttggt caggacggtc acggtagcgg
    33601 tttcttcttt actgaaaaac cggatgctaa tgtctgggtc gatggcgcag tttcaagcta
    33661 ttaccgcgaa acctatgccg aagcagaaca acgtttaggt gaagttcgcg ccctgcgtct
    33721 ggcgggtcat aacaatatat tccccacgct ttcatggcta aacggcactg ccacactccg
    33781 cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa
    33841 agccgcctcc gatgaagtta aagccgcttt cgaaaacagc gccactcgtg cttttggtcc
    33901 tgctggtttt ctcgagcagg atgactcgga gaactggtgt gaaatccaga aattgctgaa
    33961 aggtcaccgc gcccgcaaca gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg
    34021 tcgcgacgac agcattccag gcattactaa ctatatcttc tcagaaacgg ccgctcgtgg
    34081 aatgtaccaa cgctgggccg atctcctgag tagcgaaagc tggcaggaag tgctcgataa
    34141 aaccgccgct taccagcagg aggtgatgaa atgagtgcgc aagtttcact ggagttacat
    34201 caccgcatta gccagtttct ctttcacgaa gccagtctgc tggatgactg gaaatttcgt
    34261 gactggctgg cacagctcga cgaagagatt cgttacacca tgcgcaccac ggttaacgca
    34321 caaacgcgcg accgccgcaa aggcgttcaa ccgccgacaa cctggatttt taatgacacc
    34381 aaagaccagc tggaacggcg aatcgcccgt ctggaaaccg gcatggcctg ggcagaagaa
    34441 ccgccgtcac gcactcgtca cttaatcagc aactgccagg taagcgaaac cgacatccca
    34501 aacgtatttg ccgtgagggt gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa
    34561 acattctatg ttggaacgcg tttcgacaaa gttcgccgtc tggaggacgg gaactggcgc
    34621 ttgctggaac gggatatcgt cctggatcaa gcggtcatct cctcccataa cctgagtgta
    34681 ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc
    34741 tccggatcga tacctcgccc gtcatcgccc tgttcaacgt aggcggcgag ttttatgcca
    34801 ttaacgatcg ttgcagccat ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg
    34861 ccacggtgga gtgcccgctg cacgccgcca gtttttgcct gaaaacgggg aaagcgttat
    34921 gcctgcccgc caccgatccg ctaaccactt atccagtaca cgttgaaggt ggtgacatct
    34981 tcatcgactt accggaggcg cagccatgag cgatctgcat aacgagtcca tctttattac
    35041 cggcggcgga tcgggattag gcctggcgct ggtcgagcga tttatcgaag aaggcgcgca
    35101 ggttgccacg ctggaactgt cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga
    35161 acatattctg gcggtggaag gtaacgtgac ctgctatgcc gattatcaac gcgcggtcga
    35221 tcagatcctg actcgttctg gcaagctgga ttgttttatc ggcaatgcgg gcatctggga
    35281 tcacaatgcc tcactggtta acactcccgc agagacgctc gaaactggct tccacgagct
    35341 gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat
    35401 cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc gcctggtatc ccggcggcgg
    35461 tggcccgctg tacaccgcca gtaaacatgc cgcaaccgga cttattcgcc aactggctta
    35521 tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg tgtggtatgg ctagcgacct
    35581 gcgcggccca caggcgctcg ggcaaagtga aacctcgata atgcagtctc tgacgccgga
    35641 gaaaattgcc gccatattac cgctgcaatt tttcccgcaa ccggcggatt ttacgggtcc
    35701 gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc
    35761 tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg gctgggctgg atctttaagg
    35821 aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc gggcaagcgg cggcaatggc
    35881 tgcggcctcg ctacgccagc aagggttcac cggtgagctg catctgtttt ccgatgagca
    35941 acatcttcct tatgaacgcc ccccgctctc gaaatccatg ttgctggaag attccctgca
    36001 gctacagcag gtgttacccg ctcactggtg gcaggaaaac aatgttcatc tgcattccgg
    36061 tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta gtgttaacca acggcgaaag
    36121 ctggcactgg gatcagcttt ttatagcaac cggcgcggca gccagaccgc tgccgttgct
    36181 tgatgcactg ggagaacgct gctttaccct gcgccatgcc gacgatgccg ccagactgcg
    36241 agaagttctg cagcccgaac ggtcagtcgt gattgtcggt gccggaacta ttggtctgga
    36301 actggctgcc agcgccacgc agcgtggatg taaggtgaca gtgattgaac tggcggcaac
    36361 tgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccatcagca
    36421 ggctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt
    36481 agaactgacg ctgcaaagtg gcgaaacgct tcaggccgat gtggtgattt acggtattgg
    36541 tatcagcgcc aacgatcaac tggctcgcga ggccaacctt gatactgcca atggcattgt
    36601 cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat
    36661 cacccgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca
    36721 ggcgcaaatt gccgctgccg caatattagg gctaccgctt ccgcgactgc cgccgccgtg
    36781 gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga
    36841 ctggatttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg
    36901 cgtgcttatc ggtgcagtca cgctgaatca gggccgtgag attcgcacaa tccgcaaatg
    36961 gatccagagc ggcaaaacgt ttaatgcgaa actgctgata gatgaggaca tcgcgcttaa
    37021 atcactgtaa ccaggataat tagcgaatat ctcaacgcct ggggcgtggc gaggtaccag
    37081 agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga actcactacg
    37141 ttattttgat tttggtgctg cacgccccgt tttgttatta attgcccgta tcgccgtggt
    37201 cttaattttc attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata
    37261 tatggcctcg ctgggcgcgc caatgccaat gctggcagcg attattgcgg tagttatgga
    37321 agtgcccgcc gcaatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt
    37381 tattttctac acgctgggta cagcggtaat tggtcaccat tactgggata tgaccggcga
    37441 tgcggttggg ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcattcct
    37501 gctgttggca attaccgggc cgggggcaat ttctctcgat cggcgttaaa aacatgccgg
    37561 atgcggcgtg aacgccttat ccggcatttt ataaattaca gtaacgcaac ccgcaacgac
    37621 atctctgaag ttgttgattc ccctggtgca agcgcaatga gatcaccccc ttccgggcga
    37681 tgatggtcat ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct
    37741 ttatcaaacg ccgggtcgga aacaaagatg aaataacatg gtgcaggtgg cgtcgtttcc
    37801 atgatgatgg cataaccctc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca
    37861 aagccattgt ttacccactg gcacggcaac ggcgcgagct ggttgaaatc cagctcctgc
    37921 ggtagctgct cgcaaaactc acccgccagc cactgctccc gctccagcca ataaccgctc
    37981 gcctgcgcct gaatccgcgt ttgtggtgac aacggaaaat aaggatgcaa gccggtacca
    38041 aatggcagcg tctctgtccc ttgattgttg acagagagcg tcaccgtcag cgtatctgcc
    38101 gttaaatgaa acgcctgact tacccgatag tgataaacac cgctgcgatg ttcatacacc
    38161 agacacaagc tatcttcgct gcgcgagaca cattgccact gccccagcca gccgtcgccg
    38221 tgcagatagt gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag
    38281 acaaaacgat tgccgctcac ccggtttgca aacggcacca gcggaaaaca ggaggcatcg
    38341 gttgccacac cgcttttttt accgggacgt aagagcggcg tcgcgtcacg ccagaagcct
    38401 tcgatgacgc cgccccggtc agaaacgtcc agctttagcg gcccgtgaga taaggtatag
    38461 atggtcatca gctgactcct taatccggga agttaatcac aactttcccg catttaccgc
    38521 tcgccatcag cgcataggca tctcctgcct gttccagcgt gaagcgatgg gtaatggcat
    38581 tacgcggcca cagtttccag tcagtcaaat cgtgggcaca tttttccata tggaacaaac
    38641 tggttaccca ggagccaata atccgccgtt gatgatgcat cagatcggcg ctaacctcga
    38701 actccacttt tccggtttcc ccgatgtaaa ccacccgtcc ccagtcagca gtggattgca
    38761 gtgccagcaa gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccgccgt
    38821 gggtgagttc ggcgataatc tgcgacagac cttcggtcgt tgctaaatag ccgtgatcca
    38881 tcacccctaa ctgtttcgcc atcgccagac gttccggcag catatcaacg ccgatgatcc
    38941 tttttgcacc gcgacctttt gccagcatca tcgccatcat gccgaccggc cccagaccga
    39001 ccaccagcac gttatcgctg ccggacactt cgccgcgcaa aatcccttca taggccgtgc
    39061 caacgccgca actaataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca
    39121 gatctttttc ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag
    39181 ccgcttttcc tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac
    39241 cacagccaga aatgtgatac accagcacac ggtcgccctc tttaaaatgg cgacagcctt
    39301 gccccatcgc cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg
    39361 gtttatcggg tgccgctgcc gtggcacggt gttgatgata gatatagtgg acatcacttc
    39421 cgcaaatccc ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg
    39481 caacttcccg cagatcgacg gtcgaatttc ctggtaaata agctgccagc atcgttttca
    39541 taaatccctc attaacaata cgattaattt tcatccctgc cgcacccgcg ccaggggcgt
    39601 taaggtttag cgtttcgctt tactgctacg ctgggtcagc aagatattcg ccagcaccgc
    39661 caccacgatg atgacgccgc gcaccacctg ctggaaaaag gagttaatcc cgagcagcac
    39721 cagaccgtta ccgattagcg taatcaccaa cacaccaagc aatgtaccaa acagggaacc
    39781 gcgacctccg gaaagtgccg taccaccgac cacgaccgcg gcgatgacgt caaactccag
    39841 accgtttgcg gcacctgcgt taccggaacc gaggcgcgcc gccaacaaaa tgccggtcac
    39901 cgccgctaat aatcccgaaa gggtaaagat aagaatgcgc acccgacgaa cgttgatgcc
    39961 gcacaactgc gccgccgtag cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt
    40021 tttgcggctg atgaacacaa acagcgcaaa caacaccatc atgattagcg cggataccgg
    40081 cacaccgaga aattgtcccc ccagccagtc cagcacttcg ttttcgtcaa tcggcactgg
    40141 caaggcgttc gtcataaaca gccccattcc gcgcagggcg ctccataaac ccagcgtggc
    40201 aacaaaactt ggcacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc
    40261 gcctaacagc aacaccagca gacaagcaat cgccagcgga acctcaaatt gcagcaaaaa
    40321 tgccaggcac accgagacaa aagccaccat cggcccaacg ctgacatcaa tttccccgga
    40381 gataataatc agcgtcatcg cccaggcggc gatcccaatg gtggcggcat cgcgcagcac
    40441 gttcatctgg ttattcaatg agataaaacc cggcgcgttc agagagaaga ccagataaag
    40501 aatggcgatc accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag
    40561 cgagacgctc ttgccctgcg gcaatggtaa tgacgaagca gacatcttca ggttcctcgg
    40621 taaaattcat cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggagc
    40681 gtgaaactcc tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg
    40741 caactcctcc acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg
    40801 gacaatacgg taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag
    40861 caacaaaatc tggctggcag catagaccca acgaccgatc accacttttt gctgattgcc
    40921 accagaaagc gtgccgatgg gcgtttcgct actggcggcc ttgaccgtca tccgctgcat
    40981 cacctcttcg gtcaggcggc ggatggtgga ccattgcagc acaccgttgg cgctgatttt
    41041 ttgccgattg gtcagcactg tattttcgtc aacgcccagc cagggaatga tccccgcttc
    41101 tttgcggttt tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg
    41161 agtgattttc tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaactat
    41221 ggctttcaac aattcactgc gccctgcccc cagcaggcca gcaatgccga gcacttcgcc
    41281 acgacggagc gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc
    41341 cagcacagcc tgatcgacaa tttcctgagg ggaaaccggc gcaatatcaa cgtgatcgcg
    41401 cccgagcatt agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc
    41461 cacctgacca tcgcgcataa cggtggcaca ggaggcaatg cggcggattt cttccatccg
    41521 gtggctgaca taaatcaccg ccacgcccag tgccgacatc tttttaaccg cgctgatcac
    41581 cagttcaact tccgcactgg caagcgaact ggtaggttca tcaagaatga ccacgcgcgg
    41641 ctcgcccttc atcacccgcg caatttccac cagttgcttt tgcgccgggc ttagcgttga
    41701 aacaagttgt tcagggctaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg
    41761 cgccatttgc aggtaatcaa tcatgccgtt gcggcgtggc cactgaccga ggcagaggtt
    41821 ttccgccacc gtcagtccct ccaccagact taactcctga taaaccgcac gaacccccag
    41881 ttcagcggca cggcgagtca gcgtagcttc gtcaccttcc agtcgcgtct cgccaatcca
    41941 gatatcaccg ctatccggac gttcactgcc ggtaagcatt cgaatgaggg tcgatttgcc
    42001 cgcgccgttt ttgcctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt
    42061 atccaacgca acgacgccgg gataacgctt atttcctgcc accacttttg ctaccgggac
    42121 tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa
    42181 cgtgcgttgc cagccactgt ttaccgtctt ccgttttggt atagagatcg ataggcacct
    42241 gaatcacttt tgcaccgtcg gcttgtttat tgataacctt caaagtttgc gcgaaaacag
    42301 cattgcccat tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca
    42361 gctcctgagc aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat
    42421 tttgattacg taccgctttt accgcgccga gtgtcgcacc gcccgattcc cccataatgg
    42481 cgttgagatc cggcgtggag ataattagtt tttcaccaac ggaaatcgct ttatctaaaa
    42541 cagtcccttc ctgattagcg actatttgcg cgccgggaac gcgggatttt aatacttctt
    42601 caaatccttt acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt
    42661 tcggctggtc aattttattg gcaataaaat aatcggcggc agcgttaccc agttttttac
    42721 caaattccag cggatcgccg accagatacg ccgagacata tttatcgaca cctttttgat
    42781 taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac
    42841 gactactgcc attttcagac actgccgata aaataatggc atcgacatta cgcgccacga
    42901 gggtatcaac aaaggtactt tctttcgaaa tatcaccctg ggcgttagtt tcaattaact
    42961 gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcacg ccagcgtaat
    43021 atccctgggt atcaaggtat attgcgccaa tggtcatttc tttatccgca gccctggcaa
    43081 atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt
    43141 taggcatagt acctcctgta gggtttttat ttacaacggc ttattttaat tatttgagcg
    43201 tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acggagttaa
    43261 aaataaaagg catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg
    43321 tcaattcagc gcgagcagat tcatagtgcg caacctcccc gagtcccgcg gacgccagca
    43381 tcgccaccat caggcatttt tcattatgct gctgttgtaa atcgccatac agcgacaaca
    43441 ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg
    43501 cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca
    43561 gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtgaatgt
    43621 taatggtgcg atcgccagtc gccgccagac gtaaacaacg catcgcttca gtttgatcgc
    43681 cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta
    43741 aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct
    43801 gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact
    43861 gactggtgat cttcccttcc ccgccttccc acgggtggaa tttgcgcgtg gcgagaatgt
    43921 ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt
    43981 cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg
    44041 tggctccgct taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg
    44101 caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt tgcttattcc
    44161 acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac
    44221 gttgccataa agtaatggct ttgttgtagc tgcgcttgtt gtagtagaag caggccagta
    44281 aatgacgggc aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat
    44341 tcgggaaacg gacaaactgt gggaagacat caatggcttt tgtgactagt tcgccgcgtt
    44401 cagctttcgg cagcaagctg gcttgcaagt aaagcggcag cgtgcgctgg cagtccagtg
    44461 cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca
    44521 tcagggcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc
    44581 tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca
    44641 gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat
    44701 gcaggcaaag cacttcctga ttggttgggc aggcgcgaag actttgttgg caaaaatcca
    44761 gaccagcgtc gaagtgacca ttacgcgccg ccagccgtgc cagaccataa tagccacctg
    44821 ctttactgtt gccgctccag accgcccgcc agaaatcctc ttcggcttgt tgatattgcc
    44881 cctgacgttc gtaagcactg gcgcgaatca aactcgcctg tccgcactgc ggatttttgt
    44941 ttagcgcatg tgcgcgtttc agcgcctgag tggcatacgc caccgcttgc gggaaatctg
    45001 ctcggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc ggatccagcg
    45061 ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat
    45121 gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt
    45181 tggcgacgtc cggcaacggc aactcttgcg gctgatgttc ctgataactc agtatgatat
    45241 tgccgtcggc atcggagagt tcaatggtca gcctttcagg attgataccg tgcaacacgc
    45301 cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc
    45361 cgatttcgcg gatcgccaga cggtatcctt tcaacggaga gatggcatac agcccccact
    45421 caatcccccg ctcgctacgc tggagtttaa tcaccgcatc gcgggaggcg ttttgcacca
    45481 tgcccaaaga atggtaaggc aggaaatact gctcaaaacg cttctcttcg taagcatcaa
    45541 gccaggtaaa atcgggctgg ttatcggcaa aaataccagt catcagttcg atatacgggc
    45601 cgttattgtc ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccagctcc
    45661 actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat
    45721 cgtgacacca cgcgccaaca aaatcgtact gtgatttttc agccatatat gaggttggaa
    45781 caggcacatt tttatagcga gaaatgtcca ctccagcgga gtagtccact ttgtagtaag
    45841 tgccggtggc gatggggaaa gcagagacgg cccgtttgcc gtgatcaaac accgccgtta
    45901 catccggcgg gaagacgctc tgatgccctt cacccccttt cactgccggg ttggcccacc
    45961 acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagcgccg
    46021 cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
    46081 cgcctaccca caccgtctgt gcgccatctt catgggcttc gagggtgaaa tcgacgggca
    46141 taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg
    46201 gccccagcag ccccaccagc gccggtttaa tgacttcatt atgatagaca aaatcgcgtt
    46261 gtttcacttt atcccacgcg cgatgcaccc gaccgcccaa ctccggcagg atcatcactt
    46321 tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca
    46381 gcgtatcggt cacgccgtag ggataaaccg tgccggacga tccctgataa acgcgatttt
    46441 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
    46501 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
    46561 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
    46621 aactaaacaa ggtttagtga agatgagagc ctgcattaat aatcaacaga ttcgccacca
    46681 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
    46741 ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
    46801 cgaaaaacgg gtggtgaata tcgacgatga aagccagacg cgcgggcata gtagcggtac
    46861 atggctgatt gcgccggaag gtgactggac gctgtgtctg aacgtaacgc ccaccagtat
    46921 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggt gaatttgagt atttacagat
    46981 tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
    47041 taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacggtc aggttgatcc
    47101 ggtgactggc gtgtcgcaaa ccatgccgca agcgccgtgg gcaacaccgg ttgaggtgaa
    47161 atatctgctg gaagagaagc tgggcattcg ggtgatggtc gataatgact gcgtgatgct
    47221 ggcgctggcg gaaaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt
    47281 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gctgtttgta
    47341 tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgcgg
    47401 acgttatggc tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac aggcgcgggt
    47461 atggctaaaa tcacaaccgg ttaatactca gcttgatcct gaaaaactga caacagcgca
    47521 gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc
    47581 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    47641 gttgtacggt cgcagttgtg cctttggtga gaactggctt aataccatta ttcgccagac
    47701 gggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacga aaattggctt
    47761 tgggcaatta agccgcgcac aacaggtgct ggggattggc tatttgtatg ttgaggcgca
    47821 gttacgatag atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttttttc
    47881 aacagattaa ccgtttcgct tatgcgtaaa ccgggtaacg tgcacagatg tcgagaactt
    47941 tacctttgat gcgctcaata accgcttcat cattgatgct gtccagcacg tcacacatcc
    48001 agccagccag ttctttcgct tcggcttctt taaagccacg acgggtaatc gccggagtac
    48061 ctacacgaat accggaggtc acaaacgggc tcttcggatc gttcggtacg ctgtttttgt
    48121 tgacggtgat gttagcacgg cccagagcgg cgtcagcttc tttaccggtc agatttttat
    48181 caaccagatc aaccaggaac aggtggttat cagtaccgcc ggaaaccact ttgtagccgc
    48241 gctcgaggaa cacttctacc atcgctttag cgtttttagc gacctgctgc tggtaagttt
    48301 tgaactcagg ctccatcgct tctttcagag caaccgcttt accggcgatt acgtgcatca
    48361 acggaccgcc ctgaccacca gggaaaacag cagagttcag ttttttgtac agctcttcac
    48421 taccaccttt cgccaggatc aggccaccgc gcggacccgc cagggttttg tgagtggtgg
    48481 tagtcactac gtgagcatga ggaactgggt tcgggtaaac gccagcagca accaggcccg
    48541 caacgtgcgc catatcaacg aacaggtaag caccgatgct gtcagcgatt tcacgcattt
    48601 tcgcccagtc caccacgccg gaatatgcag agaagccacc gataatcatt ttcggtttgt
    48661 gttctttggc ttgtttttcc agatcggcgt agtcgatatg accgctagca tcgataccgt
    48721 aaggaacgat gttgtacagt ttaccggaga aattaaccgg agaaccgtga gtcaggtggc
    48781 cgccatgcgc caggttcata cccagaacgg tatcacctgg ttccagcagc gcggtgtaca
    48841 ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt agcgtagtca gcaccgaaca
    48901 gttctttcgc acgatcgatc gccagttgtt caacgatatc aacatactcg caaccgccgt
    48961 agtagcgttt gcccggataa ccttcagcat atttgttggt cagctgagaa ccctgcgcct
    49021 gcattacgcg cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc tcttcctgac
    49081 gtactttttc ctgctccata gcctgccaca gttcggcatc ataatcggca atgttcattt
    49141 cacgctttaa catccgcatc tcctgactca gctaacaata aaatttttgg cctttatagg
    49201 cggtcctgtt ggacaacggc gaacagtata accgaatcat tgtgcgataa caggtcttga
    49261 caaaggaatt tacgcaaacg attaccttca ggctgcgcaa ggctttggag aataaagagc
    49321 ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca aatttttcac ttcatcacat
    49381 tctttctgaa aaacaccaaa gaaccattta cattgcaggg ctatttttta taagatgcat
    49441 ttgagataca tcaattaaga tgcaaaaatt tgagatacat caattaagat gcaaaaaaag
    49501 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
    49561 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
    49621 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
    49681 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
    49741 gtagaaaaaa tcgcgcagaa gcacaccagt ttccagatca aaccggaaca gtacaacatc
    49801 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
    49861 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
    49921 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
    49981 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
    50041 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
    50101 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
    50161 cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggctgca caatcacgcc
    50221 aatgttggcg atgtcgtgaa actggtcgct ccagcaggtg atttttttat ggctgtcgca
    50281 gatgacacac cagtgacgtt aatctctgcc ggtgtcggtc aaacgccaat gctggcaatg
    50341 ctcgacacac tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
    50401 aatggcgatg ttcacgcctt tgctgatgaa gttaaggtac tggggctgtc actgccgcgc
    50461 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
    50521 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
    50581 cagttctatc tctgcggccc ggttggcttc atgcagtttg ccgcgaaaca gttagtggat
    50641 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtga
    50701 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
    50761 tcttcttcac cggtacggat acgaatgacc cgtgccacgt caaagacgaa gattttaccg
    50821 tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg
    50881 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
    50941 ccgcggtaca gctcggtatg gcctttctgg cggccaaaac ctttcacctc agtgaccgtc
    51001 atgccggtaa tgccgacttc agccagtgct tcgcgaacat cgtccagctt gaagggttta
    51061 ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc
    51121 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
    51181 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg
    51241 ttgcctttgg tgatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc
    51301 tcaacaaagg ttggcagcgc cgtgttttcg ccctccagcg cctgctctac taatgcatcg
    51361 ctaatcaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgt
    51421 acattacccg gccagctagc ggtcatcagg cgtttcatcg catcggtgga gaacgcgcgg
    51481 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg
    51541 tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag gcggtaatag
    51601 agatcttcac ggaattcccc gcgcgccatc gcttttggca ggtcgcggtg agtggcggaa
    51661 ataatccgca cgtcgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
    51721 tgcaacacgc gcagcagttt aacctgcaac ggtgcgggca tatcgccaat ctcatcgaga
    51781 aatagcgtac cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
    51841 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcacca
    51901 cagttaatag caataaatgg tttgctattg cgcgggctag cgttgtggat agcctgggcg
    51961 aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctaacgtc tgattgcgcc
    52021 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aatcgcctcg
    52081 cgccagcgtt catcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat
    52141 agcgcgtctt tatcgacagg cttggtgagg aaactgaaca ctccctgctg tgttgcagca
    52201 acggcatcgg gaatagaacc atgtgcggta agaataatta ccggcattcc cggctgcact
    52261 ttctggattt cagcaaacag ctgcatacca tccatttcat ccatccgcag gtcgctgata
    52321 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcaccact ttccgccgtg
    52381 accacgctgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
    52441 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
    52501 tggcgcgggc ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
    52561 ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg tgagttccag
    52621 ttgctgctgt agaacgtgat gttgctggcg caaagtatcc agctcgctgt cgctggactg
    52681 ctggagtttg ctgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
    52741 gcgccaaagt tgatacagag ggcgaacctg tgccgggatt tcggtactta gcgcatcaat
    52801 gcgcgcgacc agctggcggc gctcatacgg cgtaattttg gcatcggcga gcaaaatccc
    52861 ttgtttaaag gtattttgcc agctgccgtc gttatattgg cgagcttgct gacgcgactg
    52921 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    52981 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    53041 ttgataaacc ggaatttttt cttctgctgg cgtattaatg gctggcttat tgtgactctg
    53101 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
    53161 tcgttgaaaa atgtgtcgca tattcaccag acttaatgcc tatcccagtg ggcgtaattg
    53221 ttgcaggcag tttggacatg gacagcgcgg agaaaccgga gcgtacacgt cgtacgtgag
    53281 gatttcgagc actgcccggg gccgaactgg caaataaaat agcctgatgg gataggctgt
    53341 tagcattatt tcgtgttttt cgacgacggc aattcaatgc ggaaacaaac gtcttgcccg
    53401 ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt
    53461 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccttg aaaaaagggt
    53521 tcgaagatca tggcgcgttc ctcttgcgga atgggtgtgc ctgtattgat gacatcaata
    53581 taaacccgcg caccatgtaa gctgctgcga aggcaaatgt taccggattc agccccgtag
    53641 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
    53701 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
    53761 tgagcagaaa ccaccatttc caccagtggc gctaactcaa cattctccag ttccaccgca
    53821 ccgtccgcct gtttacggtt gtaatcaagc agttgttcga tcaacttttg caaattgcgg
    53881 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccaacaacc
    53941 tggtcagcca gtaattcagt gccctcacgc atactcgcca gcggcgtttt taattcatga
    54001 gataaatgtc ttaagaattg atgacgttgg gattccagcc atgacaggcg ctcacttaac
    54061 caaagaatac gttgcccaac cgagcgtaac tcactcggtc cactgaacga aacgctattg
    54121 cccagagaac gcccttctcc caaccggttg atcatcctct cgatattttt taccggaccg
    54181 ataatcatcc gcgtgaaaag cagtaccatc accagactca ccagaaatag caccagcgat
    54241 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
    54301 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttccaga
    54361 cgcgcggcgg cggcggcatc gggaccgctg ttgttacact gaagttgagc cagattgttc
    54421 aagtcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
    54481 agcatttcgc tataacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
    54541 agcacgcaat actgacggta gctgcgctcc atttccagtg ccgcgttggt catcgcttca
    54601 ctgcgccggg catcaacaag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    54661 ttcaggcttt gccaggcttg ccatgccagc accaacaggg gtagcagaat cagcaaaaat
    54721 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    54781 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    54841 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    54901 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    54961 gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactggtta
    55021 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgct
    55081 gctttgaggg atttattgct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    55141 aaagagacaa cttaagataa acttattaga taatattaaa atcaataagt ta
//
LOCUS       DARNVM010000011        50278 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.011, whole genome shotgun sequence.
ACCESSION   DARNVM010000011 DARNVM010000000
VERSION     DARNVM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.011"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..580
                     /gene="comR"
                     /locus_tag="SI621_000647"
     CDS             <1..580
                     /gene="comR"
                     /locus_tag="SI621_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HEI4063401.1"
                     /translation="FDRDAALDKAMKLFWQHGYEATSLADLVEATGAKAPTLYAEFTN
                     KEGLFRAVLDRYIDHFAAKHEAQLFCEEKSVETALADYFAAVANCFTSKDTPAGCFMI
                     NNCTTLSPDSGDIGNTLKSRHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCII
                     QGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(642..1181)
                     /locus_tag="SI621_000648"
     CDS             complement(642..1181)
                     /locus_tag="SI621_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEI4063402.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDGNGQLILNNKV"
     gene            complement(1408..2712)
                     /gene="ndh"
                     /locus_tag="SI621_000649"
     CDS             complement(1408..2712)
                     /gene="ndh"
                     /locus_tag="SI621_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HEI4063403.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3043..3585)
                     /locus_tag="SI621_000650"
     CDS             complement(3043..3585)
                     /locus_tag="SI621_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4063404.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3608..4633)
                     /gene="nagZ"
                     /locus_tag="SI621_000651"
     CDS             complement(3608..4633)
                     /gene="nagZ"
                     /locus_tag="SI621_000651"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEI4063405.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVCQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4644..5468)
                     /gene="thiK"
                     /locus_tag="SI621_000652"
     CDS             complement(4644..5468)
                     /gene="thiK"
                     /locus_tag="SI621_000652"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HEI4063406.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5449..6090)
                     /gene="lpoB"
                     /locus_tag="SI621_000653"
     CDS             complement(5449..6090)
                     /gene="lpoB"
                     /locus_tag="SI621_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEI4063407.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6104..6481)
                     /locus_tag="SI621_000654"
     CDS             complement(6104..6481)
                     /locus_tag="SI621_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEI4063408.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6484..6843)
                     /gene="hinT"
                     /locus_tag="SI621_000655"
     CDS             complement(6484..6843)
                     /gene="hinT"
                     /locus_tag="SI621_000655"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HEI4063409.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7177..9366
                     /gene="fhuE"
                     /locus_tag="SI621_000656"
     CDS             7177..9366
                     /gene="fhuE"
                     /locus_tag="SI621_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HEI4063410.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIVLALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9426..10859)
                     /gene="ptsG"
                     /locus_tag="SI621_000657"
     CDS             complement(9426..10859)
                     /gene="ptsG"
                     /locus_tag="SI621_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HEI4063411.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11153..11950)
                     /locus_tag="SI621_000658"
     CDS             complement(11153..11950)
                     /locus_tag="SI621_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEI4063412.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(11961..12965)
                     /gene="holB"
                     /locus_tag="SI621_000659"
     CDS             complement(11961..12965)
                     /gene="holB"
                     /locus_tag="SI621_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEI4063413.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHYGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(12962..13603)
                     /locus_tag="SI621_000660"
     CDS             complement(12962..13603)
                     /locus_tag="SI621_000660"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEI4063414.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13593..14615)
                     /gene="yceG"
                     /locus_tag="SI621_000661"
     CDS             complement(13593..14615)
                     /gene="yceG"
                     /locus_tag="SI621_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HEI4063415.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVSSERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14618..15427)
                     /gene="pabC"
                     /locus_tag="SI621_000662"
     CDS             complement(14618..15427)
                     /gene="pabC"
                     /locus_tag="SI621_000662"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEI4063416.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVIPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15547..16788)
                     /gene="fabF"
                     /locus_tag="SI621_000663"
     CDS             complement(15547..16788)
                     /gene="fabF"
                     /locus_tag="SI621_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEI4063417.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(16876..17112)
                     /gene="acpP"
                     /locus_tag="SI621_000664"
     CDS             complement(16876..17112)
                     /gene="acpP"
                     /locus_tag="SI621_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEI4063418.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17323..18057)
                     /gene="fabG"
                     /locus_tag="SI621_000665"
     CDS             complement(17323..18057)
                     /gene="fabG"
                     /locus_tag="SI621_000665"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEI4063419.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGTQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18070..18999)
                     /gene="fabD"
                     /locus_tag="SI621_000666"
     CDS             complement(18070..18999)
                     /gene="fabD"
                     /locus_tag="SI621_000666"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEI4063420.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19015..19968)
                     /gene="fabH"
                     /locus_tag="SI621_000667"
     CDS             complement(19015..19968)
                     /gene="fabH"
                     /locus_tag="SI621_000667"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEI4063421.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20036..21106)
                     /gene="plsX"
                     /locus_tag="SI621_000668"
     CDS             complement(20036..21106)
                     /gene="plsX"
                     /locus_tag="SI621_000668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEI4063422.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21187..21360)
                     /gene="rpmF"
                     /locus_tag="SI621_000669"
     CDS             complement(21187..21360)
                     /gene="rpmF"
                     /locus_tag="SI621_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEI4063423.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21412..21933)
                     /gene="yceD"
                     /locus_tag="SI621_000670"
     CDS             complement(21412..21933)
                     /gene="yceD"
                     /locus_tag="SI621_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HEI4063424.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22132..22716
                     /gene="yceF"
                     /locus_tag="SI621_000671"
     CDS             22132..22716
                     /gene="yceF"
                     /locus_tag="SI621_000671"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HEI4063425.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22828..23787)
                     /gene="rluC"
                     /locus_tag="SI621_000672"
     CDS             complement(22828..23787)
                     /gene="rluC"
                     /locus_tag="SI621_000672"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HEI4063426.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24360..27545
                     /gene="rne"
                     /locus_tag="SI621_000673"
     CDS             24360..27545
                     /gene="rne"
                     /locus_tag="SI621_000673"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HEI4063427.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVTEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVKPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            27678..27971
                     /locus_tag="SI621_000674"
     CDS             27678..27971
                     /locus_tag="SI621_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEI4063428.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            27968..28462
                     /locus_tag="SI621_000675"
     CDS             27968..28462
                     /locus_tag="SI621_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEI4063429.1"
                     /translation="MIQAPEPLIARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(28557..29510)
                     /gene="flgL"
                     /locus_tag="SI621_000676"
     CDS             complement(28557..29510)
                     /gene="flgL"
                     /locus_tag="SI621_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEI4063430.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(29522..31165)
                     /gene="flgK"
                     /locus_tag="SI621_000677"
     CDS             complement(29522..31165)
                     /gene="flgK"
                     /locus_tag="SI621_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEI4063431.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(31231..32172)
                     /gene="flgJ"
                     /locus_tag="SI621_000678"
     CDS             complement(31231..32172)
                     /gene="flgJ"
                     /locus_tag="SI621_000678"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEI4063432.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTAAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32172..33269)
                     /gene="flgI"
                     /locus_tag="SI621_000679"
     CDS             complement(32172..33269)
                     /gene="flgI"
                     /locus_tag="SI621_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEI4063433.1"
                     /translation="MIKFLSALILLLVTTAVQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(33281..33979)
                     /gene="flgH"
                     /locus_tag="SI621_000680"
     CDS             complement(33281..33979)
                     /gene="flgH"
                     /locus_tag="SI621_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEI4063434.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKS
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34032..34814)
                     /gene="flgG"
                     /locus_tag="SI621_000681"
     CDS             complement(34032..34814)
                     /gene="flgG"
                     /locus_tag="SI621_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEI4063435.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34951..35706)
                     /gene="flgF"
                     /locus_tag="SI621_000682"
     CDS             complement(34951..35706)
                     /gene="flgF"
                     /locus_tag="SI621_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEI4063436.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35726..36931)
                     /gene="flgE"
                     /locus_tag="SI621_000683"
     CDS             complement(35726..36931)
                     /gene="flgE"
                     /locus_tag="SI621_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEI4063437.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSDPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36956..37651)
                     /gene="flgD"
                     /locus_tag="SI621_000684"
     CDS             complement(36956..37651)
                     /gene="flgD"
                     /locus_tag="SI621_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEI4063438.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37663..38067)
                     /gene="flgC"
                     /locus_tag="SI621_000685"
     CDS             complement(37663..38067)
                     /gene="flgC"
                     /locus_tag="SI621_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEI4063439.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(38071..38487)
                     /gene="flgB"
                     /locus_tag="SI621_000686"
     CDS             complement(38071..38487)
                     /gene="flgB"
                     /locus_tag="SI621_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEI4063440.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38643..39302
                     /gene="flgA"
                     /locus_tag="SI621_000687"
     CDS             38643..39302
                     /gene="flgA"
                     /locus_tag="SI621_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEI4063441.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            39378..39671
                     /gene="flgM"
                     /locus_tag="SI621_000688"
     CDS             39378..39671
                     /gene="flgM"
                     /locus_tag="SI621_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HEI4063442.1"
                     /translation="MSIDRTSPLKPVSTVQPRENTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39676..40092
                     /gene="flgN"
                     /locus_tag="SI621_000689"
     CDS             39676..40092
                     /gene="flgN"
                     /locus_tag="SI621_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HEI4063443.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(40132..41667)
                     /gene="murJ"
                     /locus_tag="SI621_000690"
     CDS             complement(40132..41667)
                     /gene="murJ"
                     /locus_tag="SI621_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEI4063444.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41777..42700)
                     /locus_tag="SI621_000691"
     CDS             complement(41777..42700)
                     /locus_tag="SI621_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEI4063445.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKKQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42702..43349)
                     /locus_tag="SI621_000692"
     CDS             complement(42702..43349)
                     /locus_tag="SI621_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEI4063446.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPVVTAMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(43360..43944)
                     /gene="rimJ"
                     /locus_tag="SI621_000693"
     CDS             complement(43360..43944)
                     /gene="rimJ"
                     /locus_tag="SI621_000693"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HEI4063447.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44180..45388
                     /gene="mdtH"
                     /locus_tag="SI621_000694"
     CDS             44180..45388
                     /gene="mdtH"
                     /locus_tag="SI621_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HEI4063448.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            45452..46099
                     /gene="grxB"
                     /locus_tag="SI621_000695"
     CDS             45452..46099
                     /gene="grxB"
                     /locus_tag="SI621_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HEI4063449.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            46233..46793
                     /locus_tag="SI621_000696"
     CDS             46233..46793
                     /locus_tag="SI621_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4063450.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46899..47945
                     /gene="pyrC"
                     /locus_tag="SI621_000697"
     CDS             46899..47945
                     /gene="pyrC"
                     /locus_tag="SI621_000697"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEI4063451.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGGMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLIDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRNGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48019..48264
                     /gene="dinI"
                     /locus_tag="SI621_000698"
     CDS             48019..48264
                     /gene="dinI"
                     /locus_tag="SI621_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HEI4063452.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48554..48808
                     /gene="bssS"
                     /locus_tag="SI621_000699"
     CDS             48554..48808
                     /gene="bssS"
                     /locus_tag="SI621_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HEI4063453.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48923..50041
                     /gene="solA"
                     /locus_tag="SI621_000700"
     CDS             48923..50041
                     /gene="solA"
                     /locus_tag="SI621_000700"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEI4063454.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            50089..50202
                     /locus_tag="SI621_000701"
     CDS             50089..50202
                     /locus_tag="SI621_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HEI4063455.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12168 a        13517 c        12498 g        12095 t
ORIGIN      
        1 gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcaac acggttatga
       61 agcgacttct cttgcggacc tcgtcgaagc gaccggagcc aaagcgccca cgctatacgc
      121 ggaatttacc aacaaagagg ggttatttcg cgctgtactc gaccgctata tcgatcattt
      181 tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggaga ctgcgctggc
      241 tgactatttt gctgccgtcg ccaactgctt taccagcaaa gacaccccgg ctggctgctt
      301 tatgatcaac aactgcacca ccctctcccc ggattccgga gatatcggca atacgctgaa
      361 atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac gacaagcgcg
      421 cggggaaatc ccgacccact gtgacgtgac gcatctggca gaattcctta attgtattat
      481 tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactga tgcagattgc
      541 ccgaacgact ttgcgtttat ggccggaact ggtgaaataa ggcgcaaaaa aagcccctca
      601 acctgagcga atgaggggct aaattgcaga gtacagcctt gttatacttt gttattcaaa
      661 atcagttgcc cgttgccatc taacgggatc tgcgtacccg gatcgcggtc catgcggatt
      721 ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt ttctgacttg
      781 tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc ctggagagag
      841 ccctggatct ggttaccggc atagccacct cccagcgccc ccacaacagt ggcgacatct
      901 ttaccgcgac caccgccaaa ctgatgccct atcacgccgc cagcaacagc gccgagcacc
      961 gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt gacgttgcga
     1021 cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg
     1081 tattgcgggc cacgttcaaa cacgttcaga ctggccactg ccgctacgcc caacgcagct
     1141 gcgacaccaa tcccgatacc cgccaacatt gatttattca cgggaacacc tccttctttg
     1201 ctgttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc
     1261 gcgggaatgg ctggaaatac ttagggattc ggaggagtct gaaggaatgc ctgatgcgac
     1321 gcttaacgcg tcttatcagg cctaccgctc gtaccatttg tccagggcgg ataaggcgtt
     1381 tacgccgcat ccgccagtac aagccgatta atgcaacttc aaacgtggac ggataacgcg
     1441 gttaatactc cccaccagca tcattaatcc ggttttaaag taaccatgca gcgcaatctg
     1501 gtgcattcgg tatagcgaga tgtatacaaa gcgcgcaatt cgtccttcaa tcatcattga
     1561 gccgcgcgtc aggttaccca tcaggctacc gacggtggag aagttcgaca gcgataccag
     1621 agaaccgtga tctttatact gataattttt cagcggctta ccgttcatct gcgccagaat
     1681 gttgttcatt gcgcaggttg ccatctggtg tgcggcctgc gcacgcggcg gaacaaagcc
     1741 cccttccgga cgcgggcatg acgcgcagtc gccaatagcg tatatgtctg gatcgcgagt
     1801 ggtttgcagc gtcggttcca ccaccagctg gttgatacgg ttggtttcaa gaccaccgat
     1861 atctttcagg aagtctggtg ctttgatccc ggctgcccac accatcagat cagcctcaat
     1921 atattcacca tctttagtat gcaggccgcc ttcatcagca ctggtgacca tggtttgcgt
     1981 cagcacgcga acgccaagtt tcgttaactc attatgggcc gcagcagaga tacgtggcgg
     2041 taatgcaggc aaaatacgtt cgcccgcttc taccagcgtt acgttcaggg cttcgttggt
     2101 cagaccttta taaccgtagc tgtgcagttg cttgaccgcg ttgtgcagtt cagcggagag
     2161 ctctacaccc gtcgcgccgc cgccgacaat cgcaatgttc actttgccat tcgcgcccag
     2221 gttggcggag tatttcagga acagattcag catctcctgg tggaagcgac gcgcctggtg
     2281 cgggttatcg aggaaaatgc agttctcttt gacacctggc gtattgaaat cgttagaggt
     2341 gctacccagc gccattacca gggtgtcata ggcgatttta cgttccggaa ccagcaattc
     2401 acctttttcg tcgcgcagtt ctgcaatagt gattgttttc gcttcacgat caatatcaat
     2461 tacggaaccc agctggaact ggaaaccatg attgcgcgca tgggccagat agctcaacgc
     2521 atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc agcagcggtt tccacaggtg
     2581 gctgtggtta cgatcgacca gcgtgatttt ggcttttttc ttgcgtccca gcttatgccc
     2641 cagctgtgtt gccatttcca gcccaccagc accgccgccg acaatcacaa tttttttcaa
     2701 tggcgtagtc aacgtgaccc ccttaaattt attaaccaat tgttaattaa tggttattgg
     2761 catagctctt aattaacaac aggttacagc aatgaaaatg ttcttcgcta ttgagaataa
     2821 catgaatggt gcattggtca taccaaaatt gatgttaatc aagttttgtt gctgaaaaga
     2881 taggcgactt taccgggcga gatcaaaaaa cagagtttgt tgtttattat ttgagctaat
     2941 ttatgagagt ggtatagcgt gaggggtgta tggaggacgt ttggtaggcc tgataaaatg
     3001 cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga gggttttgaa
     3061 cgctttaatg cgctgtaaat gcggggagat attcttgaat ttgtgcgtct gctcttcgtc
     3121 ccagacaatc tcgtaataat gatgcagctc ttcagatgtc cgctggctgt taagcgcttc
     3181 atcattacgc gacaaaatca ccaggcaacg atcgcgattc ttttcgcgga agttggtcac
     3241 acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt ccatgttctc
     3301 ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa aaccaatccg
     3361 ttccgcccag tatccgccca gaccaacgcc gcaaatcagc ggacgctcgt cgacgttcag
     3421 ttgcaacatt ttgtccactt ctttgagcag atgctgcata tcatgtttcg gatgccgcgt
     3481 actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga ctttctcgtg
     3541 gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt cctcacataa
     3601 gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga ttcagacggg
     3661 cgctgatcgc tttccagcga gccgagtcca tcagttcctg tcgcgaaaat gaacctttat
     3721 gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac acgctgacgg
     3781 cccctttacg attattgcag accaggatca tatcgcaacc cgcgtccagt gatgcctgcc
     3841 cgcgttcggc ataactgccc ataatcgcgg caccttccat cgataaatcg tcagagaaaa
     3901 tcacgccatc aaaacccagt tcctgacgca aaacggtttt cagccaatag ggagaaccgc
     3961 tcgccggacg cggatcgaca tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt
     4021 tattttcgcg gattaaggaa ctgaagaccg acatatcttt agcgcgaatc tccgcttgcg
     4081 gacgtgggtc acacggtgtt tctttgtgtg agtctgccgt tactgcaccg tgtcctggga
     4141 agtgtttccc ggtagttttc attccggctt catgcatacc atcaataaac cggctggcaa
     4201 ttgccagggc tttttcggga tcggcatgat aagaacgctc gccaatcgcc gcgctgatat
     4261 gcccgacgtc cagcactggc gcaaagctga tatcaatatc catagcaatc atttcgctgg
     4321 ccatcaacca acctgcctct tgcgccagtt ttccgccttc ttccattccc aacagcgcag
     4381 caaatgattg tgccgctggc aagcgggtaa aaccttcacg aaaacgctgc acgcgtccac
     4441 cttcctgatc aaccgccacc accagatgat tgcgcgaagc tgcgcggatc tggcacacca
     4501 gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc cctcccacca
     4561 gcggatgcgc cagtatttca cgctcttccg cgtccagttc gtaaccttcg acatccaaca
     4621 ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca ggtgtcatcg
     4681 gccagcctga taaattgttg atcgccggtt tgtcgccaac ggtactcaaa ccaccctgct
     4741 ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg cgccggataa
     4801 atcttcgcgc gagtggcata gtcattgacc aattgccggt gctggtcagt attttccacc
     4861 cacaccgccg ccagttccag cgcgatatca ccatctcctg catactccca gtcgatgagt
     4921 tttaaccctg acgcgctatg cactaaattt ccggcgtgga catccatatg caatggactt
     4981 aagcgtaaag gccgtggttc ccgcgctttg cgcagacgtt ttaaccttcg cagccaaccc
     5041 actgtccgcc gcgccggatc gctttgctgc cagtacagtt ccagtaacgg caacagcgtt
     5101 attcgccagc caaaacgcgg ttgttgatgt agataataca gcaagcctgc cagttcgttg
     5161 gtatccggca aatacgtttt tacctcgccg ggcagatagt cgaccaccat ccagtcacgg
     5221 agatataaat gcggcttcgg tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg
     5281 cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg
     5341 ccctgatgtt caatgagaaa actcccgcca ctaagcccac tattaaacgt cgtgacggga
     5401 tgaaattgcg ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga
     5461 aacggcacct ttacctgacc agataatttc gcccgtctgc accagcatca gctgcatttg
     5521 tagggtcgga gcgttaacgt tgccagaggc actggagtat agcacgtaat gagcgccgac
     5581 attgcgggca atgcctatgg ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc
     5641 taactgttgc tttgccatcg acagctgctg ggcggaaacc agggtaaatt taccgttatt
     5701 agccagcgca tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg
     5761 gttgttaacg ctatcaacca gcaggacgct acccgcagtc acgccgtcag ccccaagcat
     5821 cttactgacc atcggctgca ttgcaccatt ccagtcataa tggcgaatat gcggcgcagg
     5881 cggtgcagtt tgatcttcgt gctcaattgg gcctggctgc tgcgggatcg tcggcaccga
     5941 gggcactgtg gggacaggct gttgtggctc ggctggttgt tccggcgctg gtttcacttc
     6001 ttctaccggt gcaggttcac gttgccccac acacccggcg agaaacatcg ccagcgcggt
     6061 aatcaaggcg tagcgactca tttttgtcat caagattcac cccttacaaa tatagataaa
     6121 gtctgacttt gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg
     6181 cgggaatggt aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat
     6241 accagtaaaa acgataatga acggtaacgg gttcttgcct ttcgttataa agcgttgagg
     6301 atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag
     6361 ccaataaaga tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg
     6421 aacgacagcc caccagcact aacaacgcca gagacaccag cccaaagcat ccttttctca
     6481 tcgttacaga cctttatgcg ccagcattgg tcccagcgga cggccaccca acaagtgcat
     6541 atggatgtgg taaacctctt gtccgccgtg gcggttggtg ttcatgatca gacgatagcc
     6601 atcttcggca ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag
     6661 cgcctgctca tgctcagcag acacgtcgtt cacagtcgga atgaggatat tcggaatgat
     6721 cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg
     6781 gtagacgata tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc
     6841 cacgacattt tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg
     6901 cgagtataga gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa
     6961 gcaaaaaatc gttttgctgc cgtatatatc gccattattc ccatttctgc ctgcgaatgc
     7021 gctgaaaaga cattctctgt ttggtttaca cgccaaacat gaatgcgtat atttctcatt
     7081 tgcatttaca tacaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc
     7141 atgagcctga cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg
     7201 gataatcaat atcaagccat caccaaaccg tcactacttg ccggttgcat tgtactggca
     7261 ctattacctt ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt
     7321 tcagccacag ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcaggt
     7381 accaaaatgc agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag
     7441 cggatggaag atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc
     7501 agcaaaagtc aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat
     7561 aactatatgg ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca
     7621 ctttctgata tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg
     7681 accgggacgg gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt
     7741 gaatttaaag gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg
     7801 gatttacaaa gcccactcac cgaagatggt aaaatccgcg cgcgaattgt cggcggctac
     7861 cagaataacg actcatggct ggaccgctac aacagtgaaa agaccttctt ctccggcatt
     7921 gtcgatgctg atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt
     7981 gatgttaata gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac
     8041 agttacgatc gcgcacgcag taccgcacct gactgggcgt ataacgataa agagatcaac
     8101 aaggtcttta tgaccctcaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc
     8161 acccactccg aagtcgaatt tgacagtaaa atgatgtatg tcgatgccta tgtaaataaa
     8221 gcggatggta tgctggttgg gccatacagt aattatggac ctggctttga ttatgtcggc
     8281 ggcaccggtt ggaacagcgg caaacgtaaa gttgatgcgc tggatttgtt tgctgacggt
     8341 agttatgaat tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa
     8401 aacaatcgtt acttcagttc atgggcaaac atcttcccgg atgaaattgg cagtttctac
     8461 aactttaatg gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat
     8521 accacacata tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
     8581 ctgatcctcg gcgctcgtta taccaactgg cgggttgata cgctgactta cagcatggag
     8641 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
     8701 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
     8761 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
     8821 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
     8881 ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc
     8941 agtaaaggtg tggaatttga actcaacggc gcaattaccg acaactggca gctgacgttt
     9001 ggcgcaacgc gctatattgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
     9061 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacggtc
     9121 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacgcc gtatggcacc
     9181 ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg
     9241 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
     9301 gtggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
     9361 ttctgaataa cacctgtaaa aaaggcagcc atccggctgc cttagtctcc ccaacgtctt
     9421 acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta
     9481 ccgaaaatcg cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
     9541 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
     9601 gcgtcgaggt tagtaatatt ttctttacca ccaaatgcag caaccagagc cggtgccatt
     9661 tcgctggtac ctgtcgcttt tgcatcttca gtcgcatctt cacgacccgg cgttttcaga
     9721 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caattgcata accgataccg
     9781 acgatcggga acagccacag tttgctgctg ttaccagaca gaacaatgaa gtcgatcaga
     9841 ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg
     9901 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg
     9961 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    10021 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    10081 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    10141 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatttgc
    10201 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg
    10261 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    10321 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    10381 gccaggccag aaatgatcgg cacaaaacgt ttaccggcaa agaagccaag atactcaggc
    10441 agcttaatac ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga gataatcccc
    10501 ccgagtacgc cagtatccgc caggtgttta gaggcgattt cttcagcagg taaatgcagt
    10561 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc
    10621 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccaatcgc aaaaatcagt
    10681 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    10741 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg cagtacggat
    10801 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    10861 attgagagtg ctcctgagta tgggtgcttt tttacgttct cacgcgtggc aaggggggag
    10921 agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
    10981 cagtgccgat ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac
    11041 attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc caattttcgg
    11101 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
    11161 tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct
    11221 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
    11281 ccatcgcagg ttgattctct tttcctcgat gcggcaccgg cgcaaggtaa ggtgagtcag
    11341 tttctaccag taaccgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat
    11401 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
    11461 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
    11521 cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt atggacgata accggcttgt
    11581 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgccgt actttagttt
    11641 ccggcgtgta ataataatcc aacccggttt cacccagcgc aacaacgccc tcttctgccg
    11701 ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
    11761 aagaaaatac gacgttgtca cgttcgccta ccagatcccg catgtgtaaa taacccggta
    11821 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
    11881 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
    11941 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
    12001 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgacg
    12061 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
    12121 gaaagatggt ttgccagttc gacgaccagc cccggcacat caacattggt cacctgcgca
    12181 gcgccataat ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc
    12241 ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga ttgcacgcta
    12301 tatgccagcg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
    12361 agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtagtgc atcctgtgac
    12421 attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa
    12481 tgtaaccgac aacgactacg taatgttgcc agtaagcgtt caggctcgcg ggtagccagg
    12541 aaaaaccaag tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
    12601 tcagtcagta aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcgtgc
    12661 tcatttaact tttcggtgac ttcacgtacc gcatcaatgc ccagcgcatt tttccctttt
    12721 tcgggagcca gggtgtaata atcgggatgc gtgccagcct gcatcaactg acatccacga
    12781 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    12841 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagcagcgc atggtgacct
    12901 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    12961 ctcatgcgtc caactccttc acccagttgg tcacggtagt gcggattgca tccatcacgg
    13021 cctccagcgg ctgggtggca tcaatggtat gaatgctttt atcttgtgct gccagttcca
    13081 gatagcgggc gcgggtgcga ttaaagaaat cgaaagattc ttgctcaata cgatccagct
    13141 cgccgcgcgc acgcgcgcgt tttaagccaa cctccggggt aacatcgaga tagagcgtta
    13201 agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    13261 taccacgtcc tccaccctga taggcttgag tggagagatc gtggcgatcg ccaatcaccc
    13321 aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    13381 aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    13441 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca
    13501 tgtcgcggat acccagttgc tcgagcgtct caaccaccac gttacgcgcg gtagttttgc
    13561 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    13621 ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca
    13681 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    13741 agcgaatccg cccccggcgt cgctatcgca cctggcggca aaccggtaat ggtataggtg
    13801 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    13861 atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    13921 aataccgagg caacctgatc gcgttcgctg gaaacggcgg tttctttttc gataattgat
    13981 gccatcgtca ccagctggtt tttatcttta tagggcagac cgtccgcacg cccttcccag
    14041 gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg
    14101 gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    14161 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt gtgcttgata
    14221 tacggggcct cacgcaattg cttgaggtaa tcgctcagac gcatcccttc taccagtcgc
    14281 agagggaact gtgcttcttt accgctttcc agcaatttca gcatctcgcg cacggtcatc
    14341 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    14401 agccattgga aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcaccgagc
    14461 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    14521 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    14581 agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    14641 gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg
    14701 gtatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcctcca
    14761 gcgaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg atacagaatt
    14821 gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttgccct
    14881 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    14941 gcgcctcatc agcgtttgtc tgctcaagat gagagcggat caatacttgc tcaagccgat
    15001 taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    15061 acgtcatccc ctcgttacgc aaacggtcgt aatgtgcggg atagaccgta acggagagaa
    15121 tccgcgttgc cggtccgctg ttcaatgtgc tgtatcctcg cccgccacta ccgcgactga
    15181 tcaccacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc tctagttcaa
    15241 gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga
    15301 tatgcgccga taacaaactg actttgccgt cgataactct ggcggtggta aaacaaccat
    15361 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttatga ccgttaatta
    15421 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    15481 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttataag ggtggaaaat
    15541 gacaatttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt
    15601 acacagcgtg tattccattc cgctaacctg acgcgcttcg tgcggtacga aatccagatc
    15661 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    15721 caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    15781 tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg
    15841 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    15901 actcgcttca atgcctgcat cacgcagagc atttgccatc gccagagctg cgcctgcgcc
    15961 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    16021 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ctagcatacc
    16081 ggcaccatcg cccagtacga aaccatcacg ctctttatcc cacgggcggc tcgccgcttg
    16141 cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg
    16201 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    16261 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    16321 cgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    16381 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    16441 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    16501 ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga caattccata
    16561 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    16621 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    16681 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    16741 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    16801 gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    16861 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    16921 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    16981 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    17041 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    17101 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    17161 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    17221 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    17281 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    17341 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    17401 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    17461 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    17521 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    17581 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    17641 cacagaaccg atagtgataa tgcgaccatg acgctttttc atcatagcgc gcattaccgc
    17701 ttttgacaga cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc
    17761 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    17821 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    17881 attcaacatc agacctttgc cgttggcacc taaataatca ctgatcgcct gagtgccatt
    17941 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    18001 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    18061 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    18121 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc actttgcccg ggccgacttc
    18181 atagagatgt tctacgcctt gcgctgccat gtattcaaca gacttcgtcc actgaaccgg
    18241 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    18301 atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac
    18361 tgccagtttg tcagctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa
    18421 cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caactgcttc
    18481 tttatgacca gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    18541 accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc
    18601 cgccatagcg cccgttcctt ccggtacggc ttcttgcata aacttaccgc gcatctcaac
    18661 cagacgcacc gcatcagcga aatcaatcac gccagcgcat accagtgcgg agtattcccc
    18721 caggctgtga ccggccatca ttgccggtgc tttaccaccc tgctgctgcc atacgcgata
    18781 cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    18841 agctggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    18901 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    18961 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    19021 acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg
    19081 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    19141 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca tgccgagttt
    19201 tttcgctgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    19261 agaacggtca agattattcg ccgtcagcgt ctcatcaacg atgtgcgcca gttctgttac
    19321 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    19381 acggtcagca ttaggcagcg tcagcaactc accatagcta ccgtcggcat gcagatgggt
    19441 ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    19501 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    19561 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    19621 ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccagcat
    19681 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    19741 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    19801 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    19861 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    19921 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    19981 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    20041 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    20101 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    20161 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    20221 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    20281 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    20341 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat
    20401 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    20461 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    20521 tactgtcgag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    20581 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    20641 tactgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    20701 gcggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    20761 ttgccagccc catcagcgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    20821 ctttcaccag ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg
    20881 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    20941 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    21001 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    21061 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    21121 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    21181 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    21241 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    21301 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    21361 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    21421 ctttaagctg gctaatacgg caaatgggtt tggcttttgc gcttcttcag gcagttcacc
    21481 aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    21541 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    21601 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    21661 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    21721 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    21781 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    21841 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    21901 atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    21961 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    22021 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    22081 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    22141 cttattttag cctccacttc gccctggcgt cgcgctcttc tggaaaaact gcaaatctct
    22201 tttgaatgtg cagcaccaga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    22261 ttggtgcttc gactggcaca agaaaaagca caatctctgg cgtcacgtta tccggatcat
    22321 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    22381 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    22441 actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt
    22501 gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg taaagagcat
    22561 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattactct gtttgagcgt
    22621 ttagaggggc gtgatcctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    22681 ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg
    22741 tcgcatccgg caatcggtgc tggcggcctg atgcggcgta aacgccttat caggccaacc
    22801 gcccattatc atgcttatat gctcgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    22861 cttcaaacca tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    22921 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgccgg cttcagtgag
    22981 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    23041 ctgtgtatgc acacggatct gatgagtacg ccctgttacc gggctacaac gcaccagggt
    23101 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    23161 gacacgcacg atacgttcgc cgctttgcag aatatttttc agtaacggcg cttgaacgct
    23221 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt
    23281 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    23341 acctgaggtg tcccggtcaa gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    23401 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    23461 ggaaggttta ttcagcacca ggatatggtc atcttcatat aagatgacgt ccgccagcgc
    23521 tgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    23581 tgggatacgt acttcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    23641 cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    23701 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    23761 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    23821 gccactcatt tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca
    23881 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    23941 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    24001 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    24061 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    24121 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    24181 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    24241 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    24301 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    24361 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    24421 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    24481 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    24541 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    24601 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    24661 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    24721 gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc
    24781 gtcgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    24841 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc
    24901 aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    24961 gcccagctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt
    25021 acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac
    25081 gccagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    25141 gcgagatccc gctgttcagc cactaccaga tcgagtcaca gattgagtcc gccttccagc
    25201 gtgaagttcg tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaaccg
    25261 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta
    25321 acaccaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    25381 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    25441 accgtctgcg tgaagcggtg cgtcaggacc gggcgcgtat tcaaatcagc catatttctc
    25501 gctttggcct gctggaaatg tcccgtcagc gtctgagccc atcactgggt gaatccagcc
    25561 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgttgtcgc
    25621 tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacactcag gaagttcacg
    25681 ccattgttcc tgtgccaatc gcgtcttacc tgctgaatga aaaacgttct gcagtgaatg
    25741 cgattgaaac tcgtcaggac ggcgtgcgct gtgtgattgt gccaaacgat cagatggaaa
    25801 ccccgcacta ccatgtgctg cgcgtgcgta aaggggaaga aactccaacc ttaagctaca
    25861 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    25921 gtaagcgtcc tgaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagctc
    25981 caaccccagc tgaacctgcc gcgcctgttg tagccccagc acctaaagct gcaacggcaa
    26041 caccagcatc tcctgcacaa ccagggttgt tgagccgctt cttcggcgca ctgaaagcgc
    26101 tgttcagcgg tggtgaagaa accaaaccgg ccgagcaacc agcaccgaaa gcagaagcga
    26161 aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg
    26221 agcgccgcga tacccgtagt gaacgtacag aaggcagcga taatcgcgaa gaaaaccgtc
    26281 gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggctg
    26341 aggtaacgga aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc
    26401 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc aaaggcgctg aatgttgaag
    26461 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    26521 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    26581 tcgcaccagt ggttgaagaa actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc
    26641 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    26701 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    26761 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    26821 caacccaatc gccaatgccg ttgaccgtag cgtgcgcatc tccggaactg gcctctggca
    26881 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaggtcgaa gagcagcgcg
    26941 aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    27001 aacctgttgt tagcgcaccg gttgttgaag aagtgactga agttgtagaa gcccccgttc
    27061 aggttgccga accgcaacct gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    27121 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg
    27181 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg
    27241 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    27301 tcgtcgccga agtcgcagca gaagttgaaa cggtagccgc ggttaaacct gaggtcaccg
    27361 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    27421 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgctg
    27481 caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    27541 agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcaggg
    27601 tagcatcttt gttgtaatga cattagcgtt acatgcgctg tataattatc atccatgcaa
    27661 agccgaagga tatatttatg ccagcagcca ataacattcc aaccaggcgc gaatctttaa
    27721 atttgcgcat aaaacccgca gaacgcgatc ttattgaccg ggccgctaaa gcccgaggaa
    27781 aaaatcgcac cgaatttgtc cttgaggccg ccagagccgc agctgaagag gcgctcattg
    27841 agcagagaat tttcaggatc gattctgatg attaccagct atttttgaat cgtctggatc
    27901 agccccccgc gcccaatgct gcattacgca aaaccatgca aacacccgcg ccgtgggagc
    27961 aaaaaaaatg attcaggccc ccgagccact cattgcaagg catcaattca cctctttttg
    28021 ctcgggagta gaaacgatgg ataactggct aaagcagaga gccctaaaaa atcagcttgc
    28081 cggcgcatca cgtacatttg ttagctgtga tacgtatagc aacgttttgg catattattc
    28141 gcttgcttcc agcgccgttg aaacgtatgt cgcgacagga cgatttcgcc gaaatatgcc
    28201 agaaccgatc cctgttgtag tacttggtcg tctggcaata gataaatcat tacagggtca
    28261 gggcatcggt cgtgcgatgg tcagagatgc cggtctacga gtactacagg cggctgaagt
    28321 cattggtatt cgcggaatgt tggtccatgc gttatcagac caggcccgtg agttttatct
    28381 gcgagtgggg tttgaaccat cgcctgtcga ttctatgata ctgatggcaa cattggctga
    28441 tttgcaggaa tgtcttaaat agttcgttat gtctcatgca aaaaaatgcc cgccccggcg
    28501 atcccagagc aggcagagaa aacataccca gtttcatgat atgttttaaa gcgagcttat
    28561 ttgttgagct ggaacagcga caatccctgc atatcggtaa atgctttata cgatgcctgc
    28621 aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga
    28681 tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc cagcgactcc
    28741 agttcgttca gctgcgtgcc taattccgcg cgaacagtca gcacattgtt cagcgagttt
    28801 ttcagtccgc ggttggtttt atccagtgcc gcagcggcgg tttctttatc cgcttcgcta
    28861 tccgcgaccg gcgtttttag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt
    28921 tcagaagcgc taccgtctgg ttccgctacc gcgttgctgg taatactgtc gaaaattttg
    28981 tcacccgtgt gccctatcac catcgaacgc gaagcatcga cctgttgttt aatactttct
    29041 gctccgcctt cgtatttgcc atccgcctca ctaaacggcg cagtttctgt tttataaccg
    29101 gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga
    29161 agcccctgaa tatccgtagc cagcgaggca cggtcatcgt cactcaaggt gccattgctg
    29221 gcgtagacaa ttttttcctg agcattctgg atagcggtgg tgacctggct aagtacactc
    29281 tcttccagtg acactttttg agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgc
    29341 gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcatc tgaagggtta
    29401 acgactcgct tacccgtcga catctgttcg ccgtacttca tccattctgc ctgagaattg
    29461 gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc
    29521 cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat
    29581 tcgcgaggta atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac
    29641 cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttga gtggtgctac
    29701 tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt
    29761 taaaggattt cgcaccgccc accgttttac tgttgctttg cagatcgagc agggcctgac
    29821 cgttgcggtt gtcgctatca cccgcatctt ctgcgctcgc catggcgatt ttcgcttcgt
    29881 cggtgattaa tacatccatg ttgacgatgg cgtcacttac cggtttcagc gtgaagctgt
    29941 cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt
    30001 tggcatccgg cgtcaccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat
    30061 tattatcgaa cgagattttg taatctgtcg ccagtaccgc ggaggcatca gttaccgttg
    30121 caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag
    30181 caaagaaatc ttcaccggca tcgccgttag catcaaatcc ggctttgtgt tgggtgttga
    30241 aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tcaagatcct
    30301 gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct
    30361 caatattgcc tgccgtccca tcaacataag cgacagtcgt acgagaaggg tcggcgctgg
    30421 aaggaactgc cgcaagttgc cgcgccgtac ttccctgaac cagtgagtag ccattggcca
    30481 tcgtgatgtt ataagtgcca ccatcctgaa cgctgacttc tacgccaaca atctggttta
    30541 attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc cctgccccca
    30601 cgcctgtcag gcgggagatc tgatcgttca ggctggcgat ttgtttagcg taattgttga
    30661 tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg cgcagatatt
    30721 gatcggtggt tttaaactga ttcaccaatc cttctgattt cccaatcagc gcctggcgcg
    30781 ctgccggatc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct
    30841 gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac atctgctcat
    30901 agcgggcggt caggccgcta ctttgcgtct gcgcagcacg taactggttg gtaataaacg
    30961 catcatactc acgctgcaca ccagaaacgt agacgccatt gccaacccag ccgccagcgc
    31021 ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg gcaacgttat
    31081 agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc agtccgctca
    31141 tggcgttatt gatcaagctg gacatggagg ttccttataa gccttcaatt acagagtatt
    31201 atcggcagcg ccccgccgga cttgagttat tcagaacaga ttatcaatgt tcatgctgta
    31261 ggttttgctc accttgtcgc ttatcgattt catctgctga atcatgttgg tgagtttgcg
    31321 ggcatagtga ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc cctgctccgc
    31381 actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgc gttaacagcc caacgtaatc
    31441 cgacaatgct tccagatacg agctgtagac ccgaaacttc gcttttactt tcttcgcttc
    31501 gccattttca tattcagtcg tggtgatttc agtgactggc cctttccagt tgccagaggc
    31561 tttgacgcca aacaggttat agctcggctc gccgttttca cggcggattt gccgttgtcc
    31621 ccagccagat tccagcgccg cctgagcgag gatcaaatga tgtggcacac cgctttgctg
    31681 gcttgccagt tgggcgggca acgagagttg cgcgaggaat gctttactgt cacccggcag
    31741 cgaatcatcg tagttacgtg gtacggcctt ttgcaccagc tgcgaaagcg tctgattttg
    31801 ataacgcacc acggtttcga gcgggaattt catcggtgct gctgccgtgg gctcctctgg
    31861 caatggttgt tctggcgtca tctgtttaac catcatctct gccagcccca gacctttgcc
    31921 cgccgtcatc tgttgggcaa tctgctggtc atacatactg gtatacaggc gagtgtgctc
    31981 gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac
    32041 gaacatccct tccacctggc gggccaccgg acggatattt gccgccggat cttcgcccgc
    32101 tttcgcctta agttcgttga gcgattgtgc atcccaggcc gcacttgcca gtagtttgct
    32161 gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact ttgcattgat
    32221 tgcagtatgg acatcagatc catcggcgta gcgcccagcg cattgagcgc acgcaccacg
    32281 ttattgaggc tggcgctaga acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg
    32341 atctgcgttt gtggagtaac cacagtctgc ccaccaccaa acggtgtatc tggctggctg
    32401 acattggcct gacgattaac tgttactgag agattcccct gcgctaccgc gcagctgtcg
    32461 agggtcactt cgcgattcat caccaccgaa ccagtgcgcg agttaatcac tactttagcg
    32521 tcctgcgggg taacattaac ctgcatattc tggatatcgg caaggaaacg gacctgggaa
    32581 ctgttgccac tcggtacgcg cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca
    32641 tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatactgaa atcttcgtcg
    32701 ttaagttgca aattaagggt attcccgacg ccaaactggc tgggcaattc acgttcaata
    32761 accgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact gctaccgcca
    32821 gcggaggccc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata cacctgactg
    32881 tcaacgccct taagcggtgt catcaataac gtaccgccac gcagactttt ggcatttccc
    32941 atggaagaaa ccaccacgtc aatggtttgc ccctgacgtc caaacggtgg aagtgacgcc
    33001 gtcaccatta ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg aacggtaatt
    33061 cccagttgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg agttgtctgg
    33121 tcacctgtac catcaagccc caccaccagg ccatagccaa tcagtgagtt ttgccttacc
    33181 ccctgaacac tggtgagatc gcgaatacgc tcagcctgaa ccgccgtcgt aaccagtaga
    33241 agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc gacagattaa
    33301 ggaagaaacg ttgcaaccag cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt
    33361 attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg ctgatagtgc
    33421 gtggattaac tacaccagag aagcgaataa attcggtacc ctgattaatg gcgatctgtt
    33481 tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg
    33541 tgccgctaaa ggtattgctg gcattggccc cgccttttcc gttgaacgtg ttaccaccgg
    33601 aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc ggcacagtat
    33661 caaagccaaa attagattta ccgtcacggc tggcattcgc agaggagctt ttgctggcgc
    33721 tgacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt cgacgatctt
    33781 caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata gaaccgttgg
    33841 cgacgggcgt cggaccggga accggttgtg cactggtcgc cccctgtacc agcggcgtgg
    33901 agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg gcataagtat
    33961 gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca gtaaaccggt gaatctgcca
    34021 ccggttatgc cttagagttg cgtcagtttt tgcagcatct gatcggtggt ggataccgct
    34081 ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc ttccgccacg
    34141 ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt caggcccggc
    34201 gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt agaggttttc gccaatactc
    34261 tccagtccgg tatcattcat aaaggtggtg agattgagct gcccaacctg aaccggagct
    34321 gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat acttaacgca
    34381 ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac cagctgcccg
    34441 ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgagccatc tggcaacatc
    34501 acctggaaaa agccctgccc tttaatcgcg acatctttgc tgttattggt ttgcgacagg
    34561 ttgccctggc tgtgtaagcg ttcagtggcg accgggcgta cccccgtacc gatttgtaat
    34621 ccggatggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat ggtttgataa
    34681 agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt tggtactaac gtttgccagg
    34741 ttgttggcaa tgacgtccat attggtttgc tgggcatcca ggcccgtttt ggcgatccat
    34801 aatgaactga tcataaaatg tcctgtagtg gattagtgac aagcagagca gaagcacgcg
    34861 acggtgcgaa tttcccctca ccctaacctc tcccaaaatg ggcgagagaa tagtccgcgc
    34921 gcttttattg caatgtcatc aatcgtttaa ttaactcatc gacaacagtt ggttggcacg
    34981 gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct
    35041 ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaactcccga
    35101 catcacacgc aaggttggat ctgcctgcag taccggccca cgcgtggcct ggctttccgc
    35161 gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag
    35221 tttcagacgc cccactggcg caaccgtatt tgccggatcg cccggattca gcgccgagat
    35281 tgtgccatcg gcagcaatag tgatttccgc cccttccggc acagcaattg gccccgcctc
    35341 gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc
    35401 attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccagc catcctgctg
    35461 caacgcaacg tccagcgggc gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc
    35521 cggcgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    35581 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    35641 ggttaccgcc tgttgattca acgtctggct ggctgctccc atcgcggtat aaattgcgtg
    35701 atccattgag ctatcccgtc agcgattagc gtaagttaac cagcgtgttg aggatctggt
    35761 cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga
    35821 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa
    35881 agttgcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    35941 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    36001 ccagcagttg ggtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt
    36061 gataactcac cagatcgccc ggtttgtagc cgttctgggt ggttgccaca atgttgttag
    36121 caccggtatt ttgctgcatg gagttgagga aactcaggct aaacgtggcg ggatctgcgc
    36181 cgttaattgc gccagtggaa atattcgctg ttggatctga ggtcagaacg ccattggcat
    36241 taaacaccaa tgttgtcgct gtcttcgcaa tgctgtttgg atcactacta tcctgggtgt
    36301 agacctgcca gttattatcc ccggtcttca caaagtagac gctcatgtca tgagcattac
    36361 cctgactatc gaaaacagtc accgaacctt ttttgttata gctatccgca ttgctggcat
    36421 caaatgcgtt aacagaggga agcggatcac tggaattcag gttgatctgc atcgacgccg
    36481 tggtggtagt tttcgctgcc atcagggtat tcggaatcga aatattggtc ggattcgccc
    36541 cttgctgaat agtcggcggc gtaccggttg ccgggtaacc cgtcagctgt aaaccttgca
    36601 tattcaccag gttacggttt tcatccagct taaattgtcc gttacggctg tagaacaccg
    36661 aaccgttgct gtccaccaga cggaaaaaac cgttctggct gatagcaacg tccagacctc
    36721 gcccggtgtt ggtggtcgtg ccatcggtaa agtcctgagt gataccggca acttttaccc
    36781 ccagtcccac tttcgaaccg gcaaacatat cggcaaaaga ggccgtgcct gatttaaagc
    36841 cgtaggtggc ggagttggcg atattgttgc caataacatc gaggttggtg gcggcagcgt
    36901 ttaatccgct aaccgcttga gaaaaggcca tgactgactc ctgaaatgtg aaggcttaaa
    36961 ttatctgccg tacttcgtcg agggtggtgg tgccgtaagt gccgagatcc agcgtattac
    37021 cgttgttgcc gcggatcaca ccctgcacca gagcaaactg cagcggctgg gcaaccagtt
    37081 gtgtaccacc gttactggcg ctaatcgcta cattgtaaga accgttcggc gcagtgctgc
    37141 cgtcagtcaa cgtaccgtcc caggtgaaac tgtggactcc ggcagtcagt tcaccgatat
    37201 caatggtacg gacaaccgca ccgtttttat cggtgatggt ggccgtaact ttgtctgccg
    37261 cctgttgcag ctcaacacca aacggcgtgg tcgtggtcac agccccttct tcactgcctg
    37321 ttcccgcaag aacagtggtg ccggggatca tcacgccgtg accgatcagg ttactggcct
    37381 gtaacgactg gctgttatca atctgtccgg aaatagatcc gagcgtggta ttgagttttt
    37441 caatcccgct gaccgtgctg atttgtgcca gttgcgacgt cagctcgttg ttttccattg
    37501 gattggtcgg gtcctggttt ttcagctgcg ccaccaacaa agtcagaaaa ctgctttgta
    37561 aatctgcggc gttgctgccc gtgagcgaac tactactggt ggtgctgacg ccggtatttg
    37621 tcggatcggt ggtggttacc gcaatggaca tagctttctc ctttattgac cgagcgtaag
    37681 ggttttcagc atcatgcttt tcaccgtgtt gagcacttca acattggcct gatagctgcg
    37741 tgacgccgac atggtgttaa ccatctcccc gacgacatca acgtttggca ttttcacgta
    37801 gccctttgca tccgccagcg gattacccgg ttcataaacc agtttgtccg gggcctgact
    37861 ttctataaca tcggcaactt ttacgccgcc tgtcgcagca cctggcgcag cgttaacctg
    37921 aaataccacc tgttttgccc gatatggctg tccatcggga ccggtcacgc tatcagcatt
    37981 cgccagatta ctggccgcca cgttcaggcg ctgggactga gcagttaacg ccgacccggc
    38041 gatatcaaaa atattcagca gtgccatccg ttaatttccg ctctgtaaaa cgttcatcat
    38101 gcctttgatt tgcccgctca acgcgctaag gctcatctgg tattgcaggc tgttatcggc
    38161 aaactgggtg cgttcgcgat ccatatcgac ggtattaccg tcaagcgaag gctggtccgg
    38221 aatacggtat tgcagttctg cggtaggagg tgtcagcgcc tgcgccggaa tgtgttgcgt
    38281 tgaggtcatc gtcagtgcaa ccacgcttgt tgcatcccgt ccacgttgca tgactttttt
    38341 aagttcactg gcaaaatcga tatcgcgcgc ctgataacca ggggtatcgg cattggcgat
    38401 gtttgctgcc agcacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg
    38461 taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca
    38521 gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta
    38581 tgccgttgat ggtcattgcg gacaggtaca attcacgttg tagatatggc tggggggcga
    38641 aaatgctggc aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagtg
    38701 cggcgagcaa tctcacatcg caattgcaca ccttttttag cgcccagctc gcgggggtaa
    38761 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc
    38821 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    38881 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    38941 ccgcaatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgcg
    39001 gacggctgga caccttgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    39061 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    39121 ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag tggtgatggg tttagcgcca
    39181 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    39241 tatcgggaca ggtggtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    39301 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    39361 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg
    39421 ttcaaccgcg cgaaaacact gacgcgccgg taacgaacac ccgggcggca aaaacaaccg
    39481 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    39541 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    39601 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    39661 agagtaactg atcgtatgac gcgtcttgca gagatcctcg accagatgtc cgctgtgctt
    39721 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    39781 ggcagccagt tgcaatggat tacagaacaa aaaagctctc tgctggcgac gctggattac
    39841 ctcgaacagt tacgcaggaa agaacccaat gcagcaaata gcgttgatat tagtcaacgc
    39901 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    39961 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    40021 gaaccgacgc tatatggagc gaacggtcag acctcaacaa cccatcgcgg cagtaaaaag
    40081 atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatacatt gttacaccgt
    40141 ccggcgggca aactctttaa ctttgaagcc cagtaccgcc agtgcagcaa agtacgcagc
    40201 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    40261 ccactccggc atgatatgta acatacctaa aagcacgccc gacatcacca gtaccgccac
    40321 caccagacgc aacagaaacg ccatccagcc aggttgcggg gtaaagattt tctgcttacg
    40381 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    40441 cccggcatgt ttcaacggac cgataaacgc aaggttcatc aattgcgtta aaatcagcgt
    40501 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    40561 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    40621 catcagcgca tcaaaggcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    40681 tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcagccc
    40741 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    40801 cggcagcaaa atcgtgccaa gcgccacgcc cagtacaccg gacggaaact ccattaagcg
    40861 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    40921 gatgattaag gagatctgac tcacagagac gccaaggatc gccggtccca tctgtttcac
    40981 cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    41041 cttcaggtgc ggtagctgat acaccagctg caggacgccg ccgaccgtaa cagcccacgc
    41101 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    41161 gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg tattcagaat
    41221 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    41281 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    41341 cacccaaggt gcagcgagca tgcctgcgac cgtcacaacc gccagcgcga gtgtcaacag
    41401 accagaaaca tacgagacaa agacccgcgt ggcgtcttca ccctgcttgc ttttgtattc
    41461 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    41521 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    41581 ggcgacaatt gcgtcacgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    41641 ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc
    41701 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg
    41761 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    41821 gcgccagcac tgcctgttcg ccggcggttt gcggaactgt ctgattttgc acgcattcaa
    41881 tgaagtgccg cgcacagccg acaaatccac gttgctcaag cgtactctgc caaccaggaa
    41941 tcggtttatt cactacaccc tgcccacgct cctcacgcca ttcgcgcata tccgtaatgt
    42001 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctggcttccg gcacggcgat
    42061 gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    42121 cgccagcgtc attagtcagt agcgtaccgc catccagaga ggctttaccg cccgacaacc
    42181 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    42241 gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgct
    42301 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    42361 ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt
    42421 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg
    42481 cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg
    42541 cataaggaat gcgccagctg tcacaaattg gcagggcttt cgcgcgcgta ggcgaccacg
    42601 caccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgcc ttttgcgcga
    42661 tgccgcctaa ccccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    42721 agcaacgaat caagacgctg tttcagttct gccacttcga tttccagagc ttcgacgcgg
    42781 gcctgtaaat caccgtcaac cgcgttcgac atagccgtta ccaccggctg atcttcaacc
    42841 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    42901 accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc aacttccgcc
    42961 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    43021 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca
    43081 aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcacc
    43141 gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc agattcactc
    43201 agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact
    43261 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aatcacgcga
    43321 gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    43381 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    43441 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    43501 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    43561 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    43621 accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg caacgccaat
    43681 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    43741 ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    43801 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg caagacgcca
    43861 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    43921 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    43981 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccagaa tgccattttc
    44041 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    44101 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    44161 ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcc aggaacctgg gtaaatattt
    44221 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    44281 tatccgcttc gttgatcaaa tgggctgggc cgccgtaatg gtcggtattg ccctcggcct
    44341 acgccagttt attcagcaag gtctgggtat tttcggcgga gcaattgccg accgctttgg
    44401 tgccaaaccg atgattgtta ccggtatgct gatgcgtgcc gccggattcg ccacaatggg
    44461 tatcgcccac gaaccgtggc tactgtggtt ttcatgcctg ctctcgggac tcggcggcac
    44521 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacagcgtgg
    44581 tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt
    44641 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg cagttttgtt
    44701 tgtgctgtgt gcagcgttca atgcgtggtt gttaccggca tggaaactct ccactgtacg
    44761 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    44821 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    44881 ggtcaacgac gtggcgggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    44941 tctgtcgtta acgttgctct atcctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    45001 acatcggttg atggctgggc tgttgataat gtcattaagc atgatgccag tgggcatggt
    45061 cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    45121 cgagcctgcg cgtgaaacct taagtgcttc gctggcagac gcaagagctc gcggcagcta
    45181 tatgggattt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggcgg
    45241 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    45301 cattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc
    45361 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    45421 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    45481 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    45541 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    45601 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gacatcgttc actatgtcga
    45661 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    45721 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    45781 tgatgagttt tctactcctg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    45841 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    45901 tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    45961 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    46021 gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    46081 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    46141 gttttcagtt ttttctgctg gcgcgtttat cgctggtgac acatgctgcg ttgtgtcagg
    46201 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    46261 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    46321 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    46381 gcgtggctga cgcccatatt gttctgacaa acctgaccag ccaaattggt cgcgaagagc
    46441 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagcc
    46501 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    46561 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    46621 cggtgatgca aacgttgctc ccctatttga accaggcatt acgcaattac tttaaccagc
    46681 aacctgctta cgtcctgcgc gaagatggca gccagggcga agcaatggcg aagaaactgg
    46741 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttactgat taatcacgag
    46801 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    46861 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    46921 taaagatccg ccgaccagac gactggcacc ttcacctccg cgatggcggc atgttaaaaa
    46981 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    47041 cgcccgtgac caccgttgag gctgccgtgg cttatcgcca gcgtattctt gacgccgtac
    47101 ctgccgggca cgatttcacc ccgctgatga cctgttattt gatagattca ctagacccta
    47161 atgagcttga gcgtggattt aacgaaggcg tgttcaccgc tgccaaactt tacccggcaa
    47221 acgcaaccac taactccagt cacggcgtga cgtcagttga cgcaatcatg ccggtacttg
    47281 agcgcatgga aaaaatcggt atgccgttac tggtgcatgg tgaagtgaca catgcagata
    47341 tcgacatttt tgatcgtgaa gcgcgcttta ttgaaagcgt gatggaaccg ctgcgccagc
    47401 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    47461 tccgtaacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    47521 gcaaccatat gctggttggt ggcgtgcgtc cacatctgta ttgtctaccc atcctcaaac
    47581 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgcgtattcc
    47641 tcggtacgga ttctgcgccg catgcacgtc atcgcaaaga gagtagctgc ggctgcgcgg
    47701 gctgcttcaa tgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    47761 ctctgcagca ctttgaagca ttctgttctg taaacggccc acagttctat ggcttgccgg
    47821 tcaacgacac attcatcgaa ctggtccgcg aagagcaaca ggttgctgaa agcatcgcat
    47881 tgactgatga cacactggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    47941 aataaaaatc ctagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat
    48001 attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    48061 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    48121 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    48181 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    48241 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg gtcgccccgg
    48301 cttttttatt ttacttacaa atttcgagta caaaatttct acacttcctc aaaataattg
    48361 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    48421 gcaaaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    48481 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    48541 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    48601 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    48661 aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
    48721 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    48781 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    48841 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    48901 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    48961 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    49021 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    49081 ttatggtgaa ggcgaaaagt atgtcccgct ggttctccgc gcgcaaacgc tgtgggatga
    49141 actctcccgc cacaacgaag aagatcccat ttttgtacgc tctggcgtca ttaaccttgg
    49201 cccggctgat tccacatttc tcgccaacgt cgcccacagc gccgaacaat ggcaactcaa
    49261 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccggaaatac gcgtcccgga
    49321 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    49381 aacctggatc caactggcga aggaagcggg ctgtgcgcag ctgttcaact gcccggtcac
    49441 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gctgacggtg agtatcaggc
    49501 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    49561 ccagcccgta cgcaaagtat ttgcctggta tcaggccgat ggtcgctata gcgtgaagaa
    49621 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    49681 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    49741 tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg
    49801 caatgtcttg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    49861 gcctgacgaa gattttatta tcgatactct acccggccac gataatacac tgctcattac
    49921 cggtctgagc gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    49981 tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    50041 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    50101 ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt tctctgggga
    50161 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
    50221 actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgag attattcc
//
LOCUS       DARNVM010000012        49540 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.012, whole genome shotgun sequence.
ACCESSION   DARNVM010000012 DARNVM010000000
VERSION     DARNVM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.012"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..452
                     /gene="zapC"
                     /locus_tag="SI621_000702"
     CDS             <1..452
                     /gene="zapC"
                     /locus_tag="SI621_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HEI4063456.1"
                     /translation="SRFARKMLTPDAFSPAGFCVDDAALYFSFEEKCRDFNLSKEQKA
                     ELVLNALVAIRYLKPQMPKSWHFVSHGEMWVPMPGDAACVWLSDTHEQVNLLVVESGE
                     NAALCLLAQPCVVIAGRAMQLGDAIKIMNDRLKPQVNVDSFSLEQAV"
     gene            complement(449..1558)
                     /gene="ycbX"
                     /locus_tag="SI621_000703"
     CDS             complement(449..1558)
                     /gene="ycbX"
                     /locus_tag="SI621_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HEI4063457.1"
                     /translation="MATLTRLFVHPVKSMRGIGLTHALADISGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            1802..3910
                     /gene="rlmKL"
                     /locus_tag="SI621_000704"
     CDS             1802..3910
                     /gene="rlmKL"
                     /locus_tag="SI621_000704"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEI4063458.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            3922..5829
                     /locus_tag="SI621_000705"
     CDS             3922..5829
                     /locus_tag="SI621_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4063459.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            5959..7212
                     /gene="pqiA"
                     /locus_tag="SI621_000706"
     CDS             5959..7212
                     /gene="pqiA"
                     /locus_tag="SI621_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HEI4063460.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7217..8857
                     /gene="pqiB"
                     /locus_tag="SI621_000707"
     CDS             7217..8857
                     /gene="pqiB"
                     /locus_tag="SI621_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEI4063461.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            8854..9417
                     /gene="pqiC"
                     /locus_tag="SI621_000708"
     CDS             8854..9417
                     /gene="pqiC"
                     /locus_tag="SI621_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEI4063462.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9673..9840
                     /gene="rmf"
                     /locus_tag="SI621_000709"
     CDS             9673..9840
                     /gene="rmf"
                     /locus_tag="SI621_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HEI4063463.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9910..10428)
                     /gene="fabA"
                     /locus_tag="SI621_000710"
     CDS             complement(9910..10428)
                     /gene="fabA"
                     /locus_tag="SI621_000710"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HEI4063464.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10497..12257)
                     /locus_tag="SI621_000711"
     CDS             complement(10497..12257)
                     /locus_tag="SI621_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HEI4063465.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLPSELLKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12443..12895
                     /gene="matP"
                     /locus_tag="SI621_000712"
     CDS             12443..12895
                     /gene="matP"
                     /locus_tag="SI621_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HEI4063466.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(12970..14022)
                     /gene="ompA"
                     /locus_tag="SI621_000713"
     CDS             complement(12970..14022)
                     /gene="ompA"
                     /locus_tag="SI621_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HEI4063467.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14379..14888)
                     /gene="sulA"
                     /locus_tag="SI621_000714"
     CDS             complement(14379..14888)
                     /gene="sulA"
                     /locus_tag="SI621_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HEI4063468.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVLASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15107..15736
                     /gene="sxy"
                     /locus_tag="SI621_000715"
     CDS             15107..15736
                     /gene="sxy"
                     /locus_tag="SI621_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HEI4063469.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15699..17861)
                     /gene="yccS"
                     /locus_tag="SI621_000716"
     CDS             complement(15699..17861)
                     /gene="yccS"
                     /locus_tag="SI621_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEI4063470.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILTFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17871..18317)
                     /locus_tag="SI621_000717"
     CDS             complement(17871..18317)
                     /locus_tag="SI621_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEI4063471.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18440..20494
                     /gene="helD"
                     /locus_tag="SI621_000718"
     CDS             18440..20494
                     /gene="helD"
                     /locus_tag="SI621_000718"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HEI4063472.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWRRWSSEMSEIASGV
                     LQQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWCKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20526..20984)
                     /gene="mgsA"
                     /locus_tag="SI621_000719"
     CDS             complement(20526..20984)
                     /gene="mgsA"
                     /locus_tag="SI621_000719"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEI4063473.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21080..21742)
                     /locus_tag="SI621_000720"
     CDS             complement(21080..21742)
                     /locus_tag="SI621_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HEI4063474.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            21915..22328
                     /locus_tag="SI621_000721"
     CDS             21915..22328
                     /locus_tag="SI621_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEI4063475.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22373..22690)
                     /gene="hspQ"
                     /locus_tag="SI621_000722"
     CDS             complement(22373..22690)
                     /gene="hspQ"
                     /locus_tag="SI621_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEI4063476.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22748..23938)
                     /gene="rlmI"
                     /locus_tag="SI621_000723"
     CDS             complement(22748..23938)
                     /gene="rlmI"
                     /locus_tag="SI621_000723"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HEI4063477.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWIFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24033..24311
                     /gene="yccX"
                     /locus_tag="SI621_000724"
     CDS             24033..24311
                     /gene="yccX"
                     /locus_tag="SI621_000724"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEI4063478.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSEELTDFRIR"
     gene            complement(24308..24637)
                     /gene="tusE"
                     /locus_tag="SI621_000725"
     CDS             complement(24308..24637)
                     /gene="tusE"
                     /locus_tag="SI621_000725"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HEI4063479.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24728..25387)
                     /gene="yccA"
                     /locus_tag="SI621_000726"
     CDS             complement(24728..25387)
                     /gene="yccA"
                     /locus_tag="SI621_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HEI4063480.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25594..25681)
                     /locus_tag="SI621_000727"
     tRNA            complement(25594..25681)
                     /locus_tag="SI621_000727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25645..25647),aa:Ser,seq:tga)
     gene            26108..27226
                     /gene="hyaA"
                     /locus_tag="SI621_000728"
     CDS             26108..27226
                     /gene="hyaA"
                     /locus_tag="SI621_000728"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HEI4063481.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            27223..29016
                     /gene="hyaB"
                     /locus_tag="SI621_000729"
     CDS             27223..29016
                     /gene="hyaB"
                     /locus_tag="SI621_000729"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HEI4063482.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELVSVQVR"
     gene            29035..29742
                     /gene="hyaC"
                     /locus_tag="SI621_000730"
     CDS             29035..29742
                     /gene="hyaC"
                     /locus_tag="SI621_000730"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HEI4063483.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            29739..30326
                     /locus_tag="SI621_000731"
     CDS             29739..30326
                     /locus_tag="SI621_000731"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HEI4063484.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKISLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            30323..30721
                     /gene="hyaE"
                     /locus_tag="SI621_000732"
     CDS             30323..30721
                     /gene="hyaE"
                     /locus_tag="SI621_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HEI4063485.1"
                     /translation="MSNDTPFNALWQRMLARGWTPISECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            30718..31575
                     /locus_tag="SI621_000733"
     CDS             30718..31575
                     /locus_tag="SI621_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HEI4063486.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLVLWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPS"
     gene            31709..33253
                     /gene="appC"
                     /locus_tag="SI621_000734"
     CDS             31709..33253
                     /gene="appC"
                     /locus_tag="SI621_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HEI4063487.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQVAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            33265..34401
                     /gene="appB"
                     /locus_tag="SI621_000735"
     CDS             33265..34401
                     /gene="appB"
                     /locus_tag="SI621_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEI4063488.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGAIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            34414..34506
                     /gene="cbdX"
                     /locus_tag="SI621_000736"
     CDS             34414..34506
                     /gene="cbdX"
                     /locus_tag="SI621_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HEI4063489.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            34586..35896
                     /gene="appA"
                     /locus_tag="SI621_000737"
     CDS             34586..35896
                     /gene="appA"
                     /locus_tag="SI621_000737"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HEI4063490.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAHSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(35884..36096)
                     /gene="cspH"
                     /locus_tag="SI621_000738"
     CDS             complement(35884..36096)
                     /gene="cspH"
                     /locus_tag="SI621_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HEI4063491.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            36382..36594
                     /gene="cspG"
                     /locus_tag="SI621_000739"
     CDS             36382..36594
                     /gene="cspG"
                     /locus_tag="SI621_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HEI4063492.1"
                     /translation="MSNKITGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            36605..36793
                     /locus_tag="SI621_000740"
     CDS             36605..36793
                     /locus_tag="SI621_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEI4063493.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            36768..36998
                     /gene="ymcE"
                     /locus_tag="SI621_000741"
     CDS             36768..36998
                     /gene="ymcE"
                     /locus_tag="SI621_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HEI4063494.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            36988..37161
                     /locus_tag="SI621_000742"
     CDS             36988..37161
                     /locus_tag="SI621_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HEI4063495.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(37210..38283)
                     /locus_tag="SI621_000743"
     CDS             complement(37210..38283)
                     /locus_tag="SI621_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEI4063496.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETTGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRSV
                     ECTGCMSCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(38355..41099)
                     /gene="torS"
                     /locus_tag="SI621_000744"
     CDS             complement(38355..41099)
                     /gene="torS"
                     /locus_tag="SI621_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HEI4063497.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTSVSQYSDLLALY
                     QQDSEISHRLQTLTQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIHLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPTKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAHAIETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLILFKQHALPLLDEIDIARASQNSEKIK
                     RAAHQLKSSCTSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALKAWLNKKDLNAI
                     "
     gene            41182..42210
                     /gene="torT"
                     /locus_tag="SI621_000745"
     CDS             41182..42210
                     /gene="torT"
                     /locus_tag="SI621_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HEI4063498.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(42183..42875)
                     /gene="torR"
                     /locus_tag="SI621_000746"
     CDS             complement(42183..42875)
                     /gene="torR"
                     /locus_tag="SI621_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HEI4063499.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            43005..44177
                     /gene="torC"
                     /locus_tag="SI621_000747"
     CDS             43005..44177
                     /gene="torC"
                     /locus_tag="SI621_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HEI4063500.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            44177..46723
                     /gene="torA"
                     /locus_tag="SI621_000748"
     CDS             44177..46723
                     /gene="torA"
                     /locus_tag="SI621_000748"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HEI4063501.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDVAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFVDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYSGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            46720..47319
                     /gene="torD"
                     /locus_tag="SI621_000749"
     CDS             46720..47319
                     /gene="torD"
                     /locus_tag="SI621_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HEI4063502.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVSELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWEWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(47412..47717)
                     /gene="cbpM"
                     /locus_tag="SI621_000750"
     CDS             complement(47412..47717)
                     /gene="cbpM"
                     /locus_tag="SI621_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEI4063503.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(47717..48637)
                     /gene="cbpA"
                     /locus_tag="SI621_000751"
     CDS             complement(47717..48637)
                     /gene="cbpA"
                     /locus_tag="SI621_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HEI4063504.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            48897..>49540
                     /locus_tag="SI621_000752"
     CDS             48897..>49540
                     /locus_tag="SI621_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063505.1"
                     /translation="MGSNIHGISCTANNYLKQAWHNIKKEHEKNQTYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTKIYSGVSG
                     GMTYTIQYVQDIDVVRVSLPGRASESITDFKGYYWYGFMEYIENINACDDVFSEYCLE
                     DENISVQPERLNTPGIFNLDAGIDLSGISFIQSEINKTYELKYAPVDGDGYCLL"
BASE COUNT        12171 a        12337 c        13199 g        11833 t
ORIGIN      
        1 gctcacgttt tgcgcgcaaa atgttgactc cagatgcctt ttctcccgca ggcttttgcg
       61 ttgacgacgc cgcgctctat ttctcatttg aagaaaaatg ccgcgatttt aatttatcta
      121 aagaacaaaa agccgaactg gtgcttaatg cgctggtagc aattcgttat ctcaaaccgc
      181 aaatgccgaa aagctggcat tttgtttccc atggtgaaat gtgggtgccc atgccgggcg
      241 atgcggcctg cgtttggttg agcgacactc acgagcaagt caatttgctg gtcgttgaat
      301 ctggcgaaaa tgccgcacta tgcctgctgg cacaaccctg cgttgttatt gcgggtcgcg
      361 cgatgcagct gggcgacgcc atcaaaatca tgaacgacag gctgaaaccg caggttaatg
      421 ttgacagctt cagcctcgaa caggcagtct aacgcgtcag cttaagcgca gtcttcggta
      481 cacagctaca gcaaagaatg gtgccatcat cgcccattgc tgattttttc agcggcgtga
      541 cttcgccttc taaaagctga acacggcaac ttccacaaat gcccgcgcgg caagaataag
      601 ggatacgaat tccctgattt tctaattgct ccagcaacac ttgttggtta tttccacgaa
      661 atgcctgtcc ctgccagtca atatctacat ttgcgtccgg ttgttgcgtg atgttggcgg
      721 tatcatcagc ggcagctgcg ccgtaaattt ttgccggagc cgttgccaga atttccacct
      781 catcgccaac gcgaatcacg ccgctattac gggcaattaa attctgacca aaatcgacat
      841 cgccgttatc ctgggcagtg cggaaagatt gcaatgtttt taatggttcg cctgccggat
      901 gtttttgccc tttttctggg ctgacggtgg tgaaaataca gcggctacaa ggtttaacca
      961 catcaaatac cacatcacca atgcgaatca ctttccagcg atcttcttcc caggctgacg
     1021 cgccggaaac caccagatta gggcggaatt gctccatttt tacactggcc ggacaacgtt
     1081 gttggagatc acgtaacgag gcttcgttag caagaaggta aggatagcca tcagcaaatg
     1141 acagaggtac agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc acccagcgca
     1201 actgcacttc gcgggagaaa aatccactta gccatttgtt gatcgcgtct ggtgcaattc
     1261 gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa
     1321 aacgaacata tgcgctactg ccatctggtg cggtgagatg caagccatca tgcacaggtg
     1381 aaggagtaaa ccgtaccatc tggggaaact ggcgagcggt aataaacgta ccgtcaggtt
     1441 ccgtgatcat aaagatgcga tcgaaggcca ggccactgat atctgccagg gcatgtgtaa
     1501 gaccaatgcc gcgcatcgat ttaacaggat gaacaaaaag ccgggttaat gtcgccacga
     1561 tgcggtcctc aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt attagctata
     1621 atgcgcaaca attttcttag cgcctgtccc attaggctat tttatttgtc agtttggccc
     1681 agggaagtgc tcgaaatcct cacgtacttg tgtacgctcc ggtttctccg cgctgtccat
     1741 gtccagactg actgcaacaa ttacgcctac tgcggtaggc tcttagagta aaagtgacga
     1801 tatgaattct ctgtttgcca gtacggcccg tgggctggaa gagctgttaa aaactgaact
     1861 ggaaaacctg ggggccgttg aatgccaggt ggttcagggt ggggtccatt tcaagggcga
     1921 cacacggctt gtttaccaga gcctgatgtg gagccgcctg gcctcgcgta ttatgttgcc
     1981 gctgggcgag tgtaaggttt acagcgattt agacctctat ctcggtgttc aggcgatcaa
     2041 ctggacagag atgtttaatc ctggcgcgac cttcgctgtc cacttcagtg gtttgaatga
     2101 caccatccgc aacagtcagt acggtgcgat gaaagtgaaa gacgcgattg tcgatgcttt
     2161 cacgcggaaa aatctgccgc gtccaaatgt tgatcgcgat gcgccggata ttcgcgttaa
     2221 cgtctggctg cataaagaaa ccgccagtat cgcccttgat ctcagtggtg atggtttaca
     2281 tctgcgtggc tatcgcgatc gtgctggtat tgcgccgatc aaagaaaccc tggcagccgc
     2341 gattgttatg cgatccggct ggcagccagg aacaccgctg ctcgatccga tgtgtggttc
     2401 cggtacgttg ttgattgaag cggcgatgct ggcgaccgat cgcgcaccag gcttgcaccg
     2461 tggacgttgg ggctttagcg gctgggcaca gcatgatgaa gccatctggc aggaagtgaa
     2521 agcagaagcg caaacccgcg cccgtaaagg cctggctgag tatagctctc atttttacgg
     2581 ttcggacagc gacgcacggg tgattcaacg tgcgcgcact aacgcccgtc ttgcggggat
     2641 tggtgaactg atcacctttg aggtgaaaga tgtcgcgcaa ctgaccaatc cgctgccgaa
     2701 agggccgtac ggtacagtgt tgagcaaccc gccttacggc gaacgtctgg acagcgaacc
     2761 ggcgctgatt gcgctgcata gcctgctggg ccgaatcatg aaaaaccagt tcggcggctg
     2821 gaatctctct ttattcagtg cctcgccgga tctgttaagt tgcctgcagt tgcgtgcaga
     2881 caaacagtac aaagcgaaaa acggtccatt agactgcgtg cagaaaaatt accacgttgc
     2941 cgaaagcaca ccagacagca aaccggcaat ggtggcagaa gactacgcca accgtctgcg
     3001 taagaacctc aaaaaattcg agaagtgggc gcgtcaggaa ggcattgaat gctaccgcct
     3061 gtacgacgcc gatctaccgg aatacaacgt tgccgttgat cgttatgccg actgggtggt
     3121 ggtgcaggag tatgcgccgc caaaaactat tgatgcgcac aaagcgcgtc agcgtctgtt
     3181 cgatattatc gctgcaacca tttcggtact ggggattgcg ccaaacaaac tggtgctgaa
     3241 aacccgtgaa cgccagaagg gcaaaaatca gtaccagaaa ctgggcgaga agggcgagtt
     3301 tctcgaagtt accgaatata acgctcactt gtgggtgaac ctgacggatt acctcgatac
     3361 cggtctgttc ctcgatcacc gtatcgcccg tcgtatgctc ggtcagatga gcaaaggcaa
     3421 agatttcctc aacctgttct cttataccgg cagcgccacc gtgcacgcgg gattaggcgg
     3481 tgcacgcagc actaccaccg tggatatgtc gcgtacttat ctggagtggg cagaacgcaa
     3541 cctgcgtctg aatggcctaa ccgggcgtgc gcatcgcctg attcaggccg attgcctggc
     3601 gtggctgcgt gaggctaatg aacagttcga tctgatcttt atcgatccac caaccttctc
     3661 taactcgaaa cgaatggaag atgcgtttga tgttcagcgc gatcatctgg cgctgatgaa
     3721 agatttgaaa cgtctgctgc gtgcaggtgg gacgatcatg ttctcgaaca acaaacgcgg
     3781 tttccgtatg gatcttgacg gactggcaaa actgggactg aaagcacaag aaattacgca
     3841 aaaaacgctc tcccaggatt tcgcccgtaa ccgccagatc cacaactgct ggctgattac
     3901 cgcagcctga aaggaatagt aatgtcatta atcagtatgc atggcgcatg gctgtcgttc
     3961 agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg aagataacga acgtgtttgt
     4021 ctggtgggcc gcaacggcgc aggcaaatcg acgttaatga aaatcctcaa ccgtgaacaa
     4081 gggctggatg acggtcgcat tatttacgag caagatttga ttgtagctcg tctgcaacag
     4141 gatccgccgc gtaacgttga ggggagtgtt tatgatttcg ttgccgaagg cattgaagaa
     4201 caagcggaat acctgaaacg ctatcacgat atttcgcgcc tggtgatgaa cgacccgagc
     4261 gagaaaaatc tcaacgaact ggcgaaggtt caggaacagc tggatcacca caacctgtgg
     4321 cagctggaaa accgcattaa cgaagtgctg gcgcaactgg ggttagatcc taacgttgcg
     4381 ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat taggacgcgc gctggtgagc
     4441 aatccgcgcg tattgctgct cgacgaaccg actaaccacc tggatatcga aaccatcgac
     4501 tggctggaag ggtttctcaa aaccttcaat gggacgatta ttttcatctc ccacgaccgt
     4561 tcgtttatcc gcaatatggc gacgcgtatt gttgatctcg atcgcggcaa gctggtgacc
     4621 tatccaggaa attacgacca gtacctgctg gaaaaagaag aagccctgcg cgtggaagaa
     4681 ctacaaaatg ccgagttcga tcgcaaactg gcgcaggaag aggtgtggat ccgccagggg
     4741 atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg ccctgaaagc aatgcgtcgc
     4801 gagcgtggcg aacgtcgcga agtgatgggt accgcaaaga tgcaggtgga agaggccagc
     4861 cgctccggta agatcgtttt cgaaatggaa gacgtttgct accaggtcga cggtaagcaa
     4921 ctggtgaaag atttttctgc ccaggttcta cgtggcgaca aaattgccct gattggtccg
     4981 aatggatgcg gcaaaaccac gctgctgaaa ctgatgctcg gtcagcttca ggcggacagc
     5041 gggcgtattc acgttggcac caaactggaa gtggcttatt tcgatcagca ccgtgcggaa
     5101 ctggatcccg ataaaacggt gatggataac cttgctgaag gtaagcaaga ggtgatggtt
     5161 aacggcaagc cacgccatgt tttgggctat ttgcaggact tcctgttcca tccgaaacgg
     5221 gcgatgacgc cggtacgtgc gctttctggc ggtgagcgta accgcttgtt gttggcgcgt
     5281 ttgttcctca aaccaagcaa cttattgatt cttgacgaac cgaccaacga tcttgatgtt
     5341 gaaacgctgg aactgctgga agaactgatc gacagctacc agggcacggt attgctggtg
     5401 agccacgatc gtcagtttgt cgataacacc gttacagaat gctggatctt cgaaggcggc
     5461 ggtaaaattg gtcgttatgt cggcggttat catgatgccc gtggtcagca agaacagtat
     5521 gtggcgctca aacagcctgc ggtgaaaaaa atcgaagaag ccgccgcgcc aaaagcagaa
     5581 actgtaaaac gcagcagtag caaactaagc tataaattgc agcgcgaact ggagcagcta
     5641 ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc tacagacgca ggtggcggat
     5701 gcttccttct tcagtcagcc gcatgagcag acgcaaaaag tgctcgccga tatggctgct
     5761 gcagagcagg agctggagca agcctttgaa cgctgggagt atcttgaagc gttaaaaaat
     5821 ggtggctgat cgcaaaatag tcgatttaaa cagagacagc tgacaacggt aaaattgcct
     5881 gatgaactgg gtttatcagg catgaaaacc aacactttgt tagtcatctg taaaatagcg
     5941 catcattaag gagtaccaat gtgcgaacat catcatgccg cgaagcacat cctgtgctcg
     6001 cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg gtcagaaagc ggcatgtccc
     6061 cggtgtggca caacgttaac cgtggcgtgg gatgcccctc ggcagcgtcc caccgcctat
     6121 gcgttggctg cactgttcat gctgttgctg tccaacttgt ttccttttgt gaatatgaac
     6181 gttgcaggag ttaccagtga aattacatta ctggaaattc ccggcgtgct tttttctgag
     6241 gactacgcca gcctcggcac ctttttcctg ttgtttgtgc aactggttcc cgcgttttgt
     6301 ctgataacca ttctgttact ggtgaatcgc gcggaattac cggtccgttt aaaagagcaa
     6361 ctggcacggg tgctttttca actcaaaacc tggggaatgg cggagatttt cctcgcgggt
     6421 gtgctggtca gtttcgttaa actgatggct tacggcagca ttggtgtagg cagcagcttt
     6481 ctcccatggt gtttattttg tgtcctgcaa ctgcgcgctt ttcagtgcgt tgatcgtcgc
     6541 tggttatggg atgacatcgc cccgatgcca gaactgcgcc agccgctaaa accaggcgtc
     6601 acggggatac gtcaggggct gcgttcatgc tcctgttgta cggcaatcct tcctgctgac
     6661 gaacccgtat gcccgcgctg tagtaccaaa gggtacgttc ggcgtagaaa cagcctgcaa
     6721 tggacactcg cgctgcttgt tacgtccatc atgctgtatc ttccggctaa tattttgccc
     6781 atcatggtga cggatttatt aggctcgaag atgccgtcga cgattctcgc tggggtcatc
     6841 ctgttatgga gcgaaggatc ttatcccgtc gctgcggtta tctttctggc cagtattatg
     6901 gtgccaacgt taaagatgat cgccatagcg tggttgtgtt gggatgccaa agggcatggc
     6961 aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg ttgagtttgt aggccgctgg
     7021 tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc tggtgcgtat gggaggttta
     7081 atgagtattt atccggcaat gggtgcatta atgtttgctt tagtcgtcat aatgacaatg
     7141 ttttctgcta tgacgtttga cccacgtttg tcgtgggatc gtcaacctga atcagagcat
     7201 gaggagtcct gacagtatgg aatctaataa tggggaagcc aaaatccaga aagtgaagaa
     7261 ctggtctccc gtgtggatat ttcctatcgt cacggcgctc attggggcct gggttctttt
     7321 ttatcattac agccatcagg ggccggaagt gaccctgatc accgcgaatg cggaaggaat
     7381 agaaggtggt aaaaccacca ttaaaagccg tagcgttgac gtcggcgtgg ttgaaagcgc
     7441 cacactggct gatgatttga cgcacgttga aatcaaagcg cggctgaatt ccggtatgga
     7501 aaaattgctg cataaagata ccgtcttttg ggtggttaaa ccgcagattg gtcgagaagg
     7561 gattagcggc ctgggaacgc tgctgtctgg tgtttatatc gaactgcagc caggcgcgaa
     7621 aggcagcaaa atggataaat acgatttgct ggactcacca ccgttggccc cgcctgatgc
     7681 gaaaggtatc cgtgtgattc tcgatagcaa aaaagccggg cagctctcgc caggagatcc
     7741 cgtgctgttc cgtggctatc gggtaggttc ggttgaaacc agcaccttcg atacgcaaaa
     7801 acgcaatatc agctatcaac tgttcatcaa tgcaccttat gaccgactgg tgaccagcaa
     7861 tgttcgcttc tggaaagata gtggcattgc ggttgatctg acgtcagcgg ggatgcgtgt
     7921 ggagatgggc tcattgacaa cgctgcttag tggcggtgtc agctttgatg tgccggaagg
     7981 tctggattta gggcagccag tggcaccgaa aacagctttt gttttgtatg atgatcagaa
     8041 gagcattcag gattcgttgt acaccgatca cattgattat ctgatgttct ttaaagattc
     8101 ggtacgcggt ctgcaaccgg gagctccggt agaattccgg ggtattcgcc tgggtaccgt
     8161 aagcaaagtg ccattctttg cgccgaatat gcgtcagaca tttaacgatg attaccgtat
     8221 tccggtactg attcgtatcg agccagagcg gctgaaaatg cagcttggcg aaaatgcgga
     8281 tgttgttgag caccttggcg aattgttgaa acgtggttta cgcggatcgc tgaaaaccgg
     8341 aaacctggtc actggtgcac tgtatgttga tctcgatttc tatccaaata cgcctgcaat
     8401 aaccggtatt cgtgaattta atggttatca gattatcccg accgttagcg gcggtctggc
     8461 gcaaatccag caacgactga tggaagcgtt ggataagatc aacaaactgc cattgaatcc
     8521 gatgattgaa caggcaacca gtacgctttc tgaaagtcag cgcacaatga aaaacctgca
     8581 aacgacgctg gatagcatga acaagatcct cgccagccag tcgatgcagc agttaccgac
     8641 ggatatgcag tcaacgttgc gtgaattgaa tcgcagtatg cagggcttcc agcctggctc
     8701 cgcagcctac aacaagatgg tggcggatat gcagcgactt gatcaggtgt tgcgagaact
     8761 gcaaccggtg ctgaaaacgc tcaacgagaa gagtaacgcg ctggtatttg aagcgaagga
     8821 caaaaaagat ccagagccga agagggcgaa acaatgaaaa agtggctagt gacgattgca
     8881 gcactgtggc tggccggatg cagctccggc gaaattaata aaaactatta ccagttacct
     8941 gtggtgcaga gcggtacaca aagtaccgcc agccagggta atcgcctgtt atgggtagag
     9001 caggtcactg ttcctgacta tctggcggga aatggtgtgg tttatcaaac cagtgatgtg
     9061 aagtatgtga ttgccaacaa caacttgtgg gccagcccat tggatcaaca gttgcgcaac
     9121 accctggttg ccaacctgag cacgcaactg cccggctggg tggttgcctc ccagcctctg
     9181 ggaagcgccc aggacacgct caatgttacc gtaacggagt ttaacggtcg ctatgatggc
     9241 aaggtcattg tcagtgggga gtggctgttg aaccaccagg gacaactgat caaacgtccg
     9301 ttccgtctgg aaggggtgca aactcaggat ggttacgatg aaatggttaa agtgctggcc
     9361 agtgtctgga gtcaggaagc cgcttctatt gcgcaagaga taaagcgtct accttaatta
     9421 taaagatttg taaatataac cgcctccggt atgttgcctg aggcggtttt tttgtttcta
     9481 aggtgcggaa aaagttgttc ctcttcacat tttttgtaca actgacatgc ccgtgtagct
     9541 cacaaatatg acagtggcgt gaattttgcg cattgacggc agttatgatt cgcggtattg
     9601 cttaactgtg attgcacatt tagtaatcac tgttttcttt tccaccagaa accagtatga
     9661 gggaaacgag gcatgaagag acaaaaacga gatcgcctgg aacgggcaca tcaacgtggt
     9721 tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc cctatcagac gctgaatcaa
     9781 aggtcacaat ggctgggagg ctggcgagaa gccatggcgg acagggtagt aatggcctga
     9841 ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt tcgccttttg atactctgtc
     9901 tgattataat cagaaggcag acgtatcctg gaacagaccg actttcaggt cgctggcggt
     9961 atagatcaaa cgaccatcaa ccagcacttc gccatccgcc aggcccataa tcagacgacg
    10021 gttaacaatg cgtttaaagt gaatacggta ggtcactttt ttcgctgtcg gcagtacctg
    10081 accagtgaat ttcacttcgc caacgcccag cgcgcggcct ttaccttcgc cgcccagcca
    10141 gccgaggtag aaccctacca gctgccacat tgcgtccagg cccaggcatc ccggcataac
    10201 cggatcgcca ataaagtggc atccgaagaa ccacagatcc ggattgatat ccagttctgc
    10261 ttcaacatac cctttgtcga agttaccacc cgtttcggtc attttgacca cacggtccat
    10321 catcagcatg ttcggtgctg gcaattgcgg gcctttagcg ccaaacagtt caccgcgacc
    10381 agaggcaaga aggtcttctt ttgtatagga ttcgcgttta tctaccatgt tctctgtaag
    10441 ccttatttta ttgaagcacg caggatagct aacacgtgta cgctgaacaa gtccgatcag
    10501 ttcggaataa accagttcag ccaacgtaat ggccatggaa aacggtgacg tccttcctgt
    10561 tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca gcgtcgtttg gccttcgcca
    10621 tcccacacca gatttaataa taacggcagt gcgtcagtca catcgtctac tgcccagatg
    10681 gtgaatttgc cttcctctac cgctttcaac agttcactgg gaagacttaa atggcgaacg
    10741 ttagcagtgg ggataatgac accttgtttc ccggttaact cacgttgctg gcaaatagca
    10801 aagaagcctt cgattttctc atttaaacca ccgaccggct gggcacgacc gaactgatcg
    10861 actgaacctg tgatagcgat actctgattc accggcacat cggcgagggc gcttatcagg
    10921 gcgcagagtt cagccatcga ggcactatcg ccatccactt cactgtatga ctgctcaaat
    10981 gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta gttccgacat caggaacgcc
    11041 tgcatgatca tcatcccttt cgcatggata ttgccgccaa gctccgcttt gcgttcgatg
    11101 tcggtgaact caccatcgcc aatatgcaca acgcagctaa tgcgagaagg ttcgccaaaa
    11161 gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt taatttgccc gatgcgttcg
    11221 ccttcggttt caatcaggat ttgctcctga aggatctcat cctgcatgcg ttcagcgagg
    11281 aaaccttcgc gccattcacg ctgctgtagc attaagttta gctgctcgcc ggagaaggtg
    11341 tcgccatcgc acagggaggc gacctcttta cactggcgga ggatccactg cgggctaagc
    11401 ggaagtgttt cttgttcacc ggtgtagcgt gctgcttcgc ggataagcac cggccaggca
    11461 tccgctcccg gtgcaggtaa gtgattatgt ctggcggtca atgttaccca gcgacaccac
    11521 tggcttactg actccgcatc gacaatctgc agagtatctt caaattcgct ataaatagcc
    11581 tgctctgaaa gctctggttc catctcctgg aaatcagcca atgattcgcg ttcgcctacc
    11641 agaatgactt tcagcttcaa tggcatcgaa ggcacagaga cggggagagg gcgcgactca
    11701 tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat ttttcagccg catccacagc
    11761 agaggttgcg ccagcagtgt acgcaaagag atgatgagaa taccgccatt tgcctgatgc
    11821 accagacctg gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc aaagagttgt
    11881 tccgcttcta cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt atcatctgca
    11941 ctcactgcgt tacgtaagcg gatggtgtgc ccggaaacct catagtgacc gcccaccagt
    12001 tggcctgcat cgctttgtag cgcacgcgcg gcatcggcaa taagattcag atactcagac
    12061 tcttccgggg ccttcgccag cataaaagag gaggatgctc gcgtatgcag caactgctcc
    12121 agcgcaaatt gcagtcgcgg ttgagtatca ctgaataaag gatcgttttc gtcaatcaaa
    12181 tgtggctgag caaatatttc ctgatagcta tcggtatcag gaaccaggtc acgccatgca
    12241 agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg tcaaagccgc gattataccg
    12301 taaccggcac tgcagcacac gtagaaagca ccgacaatac tcctggcatg ggcgttaaag
    12361 ctcacaggat ggagattctt ttcttcactg gcccaaaaag ctgatattct gtaaagagtt
    12421 acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg aaagcggttg
    12481 gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaatcaccc gttacataga
    12541 agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctggaaaatg aacccgtgct
    12601 ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca tgaagcaaac
    12661 cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc gcaaaaaatc
    12721 gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcgcagc gtcgcggtaa
    12781 aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag agaaatacgc
    12841 gaataaaatg tcttctttga agcaggatct gcaggcattg ctgggtaagg aataactgac
    12901 gaaagtcagt tcaattttct atagacgaaa aaaaaccccg cagcagcggg gtttttacca
    12961 gacgagaact taagcctgcg gctgagttac aacgtctttg atgcctttaa cttcgatctc
    13021 tacgcgacga tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca
    13081 ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagattt tgtctgccgg
    13141 gataccttta gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg
    13201 gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt
    13261 cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt
    13321 cagggttgct ttgttgaagg tgaacagaac gtcagacttc agagtgaagt gcttggtctg
    13381 tacttccggt gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa
    13441 acggtaggaa acacccaggc tcagcatgcc gttgtccgga cgagtaccga tggtgtgtgc
    13501 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    13561 atactcaaca ccgccagcga agaccggaga aacgccggta tcgtggtctt taaaagatgc
    13621 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    13681 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    13741 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    13801 catttcaaag ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    13861 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    13921 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    13981 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    14041 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    14101 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    14161 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    14221 agtcttgtat aaggtacgtt taatcttttt tgtcagcgac aatttacaga agaaaatcgc
    14281 ggaaaccgct tcagacaagc ctccgcaagg aaaattagtc acgactgaaa gcattggctg
    14341 ggcgacaaaa aaagttccag gattaatcct aaatttactt aatgatacaa attagagtga
    14401 atttttagcc cggaaagttg tctcgttgcg tgagaggatg cgcttaccgg acgcataata
    14461 aaccccatag cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc
    14521 aaatcatctg ccaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc
    14581 attgactcca cggtgtggca aggggagagc tggctaatct gcatcacttt cgttaagggt
    14641 agcccagatg ccagaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc
    14701 cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg
    14761 ggctgatctt cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag
    14821 acacgcgcaa ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa
    14881 gtgtacataa tcaatccagc ccctgtgagt tactgtatgg atgtacagta catccagtga
    14941 caacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga
    15001 aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc
    15061 gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc
    15121 ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg
    15181 atcattgttt ggtagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga
    15241 tggtgagttg tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc
    15301 tgtttggctg acatataaaa agtgtggccg atccgttacc ctcaattact atcgggttga
    15361 tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc
    15421 agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat
    15481 gtcttttcat ctggaagcga ttctcgggga ggtggggatt aaggatgtac gggcgttacg
    15541 tatacttggg gcaaaaatgt gttggttgcg actgcggcag caaaacagtc tggtgacaga
    15601 aaagattctg tttatgcttg aaggggccat tatcggcatt catgaagctg cgctcccggt
    15661 ggcacgccgc caggagcttg cagaatgggc tgactctctt acgccgaaac aggagtttcc
    15721 tgcggaactt gagtaatctg gcgttgcaga cgaccaatct ctggcagtaa tgcaatcaat
    15781 aacccaactt gttgtacgac cagaggttct ttgctgtctg cccgcggttc aagttgctgc
    15841 atccgctgtt tcaggccagc taatgcctga ttgacgcgtt cttcatcagc aggttgatga
    15901 tgtagcgcgt catcaacata gcaaactgcg tcatcaagaa acgtcagaat ttcaggatta
    15961 gttaactgct cccggtgagc accgagggct gagatatagc tggtaaacgt atggttaagg
    16021 cacagcaacc gaaacgcggc ctcgcgaatt tgcggggtaa cgttcggctc gctggacata
    16081 tttgatacca ccgacgccag ttcagcatca cggttgtgtg catcgcggcg ggcaatacga
    16141 tacgccagac ggttatcacg cccctgatgg tattgctcca gtatggcatc gagatagcga
    16201 cagttggcct cggtggcacg ttcgagcatg cgcggcagat tgcgaaactt ccagtcaggc
    16261 cagatgtagc tcaccgccgc ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga
    16321 ggtaacgcca cttcaaaacc ttcacccagc aagttaaaac aaagtagcac caaaagtgtg
    16381 atgaacatcg ttgcatgggc gtattgcacg ttacggaagg caaaaaagag cacgccggtg
    16441 ataaccagta gcaccagttg cccttccagt gacggtacaa accacagcac aggaatgcca
    16501 atggcgatac ctaccagcgt accaataatc cttaacttca ggcggtggcg cgtggcgtta
    16561 tagtttggct ggcagacaaa caaacttgtc agcaagatcc aatacccgtg atgcattccg
    16621 gttatctgaa tgatggcgta gccgaagcac aacaccagcg acattcttac cgcatgacgg
    16681 aagagggcgg attccggcgt gaagtgacgg ctaagacgca gccagatatc actcaatccg
    16741 tgcgggctgt catcagcgag ctcattttcg tcattattat ggggcagtgc ctgggcctgt
    16801 tctgattcaa ttgttgccag ttgggcatca atggcgcgta aattgttcag caaaaatccc
    16861 agtgttttga gtaaatcggc aggtgcgccg ttatcgcgca tccgctccag cgcagcatca
    16921 atatgcgtaa aagcgcgctc gaaatgcgga tcatgttgat aaggctgacg caacaaaata
    16981 cagcgtgaca gttgctggca cgcctggccc tgcatcgaca tcagccgctg aaaacggaac
    17041 agcacgtcgc tgtggcgaaa atgttcacgc aatgtttgat actgaatatg agaagagctg
    17101 gcacgctcgt gaatatcctg cgcgacgaag taataatgca gcgtgcgacg cgttccccgt
    17161 tgaccacgat cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc
    17221 atcagctgac cgttggcgag agccaaatcg tacagcggtg cctggctttc atcttcaata
    17281 tcaggatcaa acatgcgcga cttgagctca aggtaacgcg ccagttgttc atagcaacgc
    17341 gccaggttgt cctgcagcgg gcggaccggg aacagtagat gaccaataag tgtcaggacg
    17401 ttgtaccaga cggcaccggc cagcagatac atcggctgct gataccagtg ctcatacagt
    17461 gatgttccca acatagtgta aatggcgatc agcaatgcac cgaaggcaat tgttgcatag
    17521 cgttgaccca gaccgccgag caaaatgaag ccgctggtag agagcgttaa gccaatcgca
    17581 aatagccagg gccagggaaa cagcaattct actgaggccg aggcgataaa aaagcagaac
    17641 agcgtaatga tgaggttacg caaacgtccc gccagtcggt catcgagatc ggtcagcgcc
    17701 gctgccacca tccccagcgt tagcggaatc gtcagtttta catcacccag ccaccacgga
    17761 aacgctgtgg ttccacaaag cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc
    17821 caggtatagc gtttgagcaa aggacttagc ataaaggcca tattagtgga ttattcaaaa
    17881 cgacgacgtg cattggcttc acgcgcagct tgcgctgttt ctaccgatac cacgcgacga
    17941 ccgaccggcc atagtgcaat agcggcaatt ttaaagttcg caatgccgac aggaatgcca
    18001 atgatggaaa tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca
    18061 aagaaaatca gccagaaaat attcaatacc gtaccgccag tatttagcag cacatttttg
    18121 ccagccgggt tcagttcatc gacatgaata gcttcattgc cataaggcac cagagacagt
    18181 ttagtgatct cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc
    18241 agagtcgcca acagccagcc cagagtggtg gcaaatccgc caagcacaaa gttcagaatg
    18301 ttcaaaacgg tacgcataaa acctcgcttt actgtggttt tcagtaatgg gcggcaattg
    18361 taacgttttt ttgggctgga gcacgttttc tctgacggtt acactgataa gaaatcattt
    18421 cgtgtggatc tacagagtca tggaactgaa agcgacaacg cttggaaaac gtctggcaca
    18481 gcacccttac gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca
    18541 cgaatacctc attcctttca atcaattact ggcgattcac tgtaagcgcg gtctggtgtg
    18601 gggcgagctg gaatttgtac tgccgggcga aaaagtggtg cgtctgcacg gtactgaatg
    18661 gggcgagacg cagcgttttt accatcatct tgatgctcac tggcggcggt ggagtagcga
    18721 gatgagcgaa attgcatctg gtgttttaca gcagcaactg gatttaattg ccacgcgcac
    18781 cggagaaaat aaatggctga cgcgtgagca aacctctggc gtgcagcaac aaatccgcca
    18841 ggctttgtcg gcgttgccgt tgccggttaa ccgactggaa gaattcgata actgccgtga
    18901 ggcgtggcgt aaatgtcagg cctggttgaa agatattgaa agcgctcggt tgcagcataa
    18961 tcaggcatat accgaagcca tgcttaccga gtatgcggat tttttccgcc aggtcgagtc
    19021 ttcaccgctg aatccggcgc aggcccgggc agtcgttaat ggcgagcatt ctctgttagt
    19081 gctggcaggt gcaggaagcg gaaaaacgtc ggtgctggta gcccgtgcag gctggttgct
    19141 ggcgcgtggt gaagcgtccc ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc
    19201 tgaagagatg gacgagcgta ttcgagaacg actacatacc gaagacatta ccgcacgcac
    19261 atttcatgcg ctggcgctgc atattattca gcagggcagc aaaaaagttc cgatagtcag
    19321 caaactggaa aatgataccg ctgcccgtca tgaacttttt attgctgagt ggtgcaagca
    19381 atgcagcgaa aagaaagcgc aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca
    19441 gtggtcagtg ccagaaggta acttctggga tgatgaaaaa ttacagcgtc gtcttgcctc
    19501 acgcctcgat cgttgggtaa gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat
    19561 tgccagtgca cccgaagaga ttcgcgatct gttcagtaaa cgtatcaagt tgatggctcc
    19621 gttattaaaa gcctggaaag gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct
    19681 tattcaccag gcgattgtga ttctggagaa aggtcgcttt atcagcccgt ggaagcatat
    19741 tctggttgat gaatttcagg atatctcgcc gcagcgcgca gcgttgttag cggcattacg
    19801 caagcaaaac agtcagacga cgttgttcgc cgttggtgat gactggcagg cgatttatcg
    19861 cttcagcggt gcgcaaatgt cgctcaccac cgctttccat gaaaactttg gtgaaggtga
    19921 tcgctgcgat ttagacacga cttaccgttt taacagtcgt atcggtgagg tggcaaaccg
    19981 gtttattcag cagaacccag gccagctgaa aaagccgcta aacagcttaa ccaatggaga
    20041 caaaaaagcc gtcacgttat tggatgagag ccatcttgac gctttgctgg ataagctctc
    20101 tggttatgcc aaaccggaag agcgcattct gatcctggcg cgttaccatc acatgaggcc
    20161 tgccagcctg gaaaaagcgg caacacgctg gccgaagttg caaatcgact ttatgaccat
    20221 tcatgccagc aaagggcaac aggcggatta cgtcatcatc gttggcttgc aggagggaag
    20281 tgatggtttt ccggctgcgg cgcgggagtc gattatggaa gaggcgctac tgccaccggt
    20341 tgaggatttc ccggacgctg aagaacggcg gttaatgtac gtggcgctga cccgggcacg
    20401 ccatcgggta tgggcactgt ttaacaaaga gaatccctct ccctttgtgg aaatactgaa
    20461 aaatctggat gtgccggtgg cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg
    20521 caatattact tcagacggtc cgcgagataa cgctgataat cggggatcag aatatcgacc
    20581 gcgtcgttga aatgcggcga ctggattatg aagtctgccg ttgccacgtt ggtggcgacc
    20641 ggaatgttcc ataccgtcgc cagacgcagc aaggctttca cgtcaggatc gtgcggcacg
    20701 gcatttagtg gatcccagaa gaaaatcaat acatcaattt tcccttctga gatcaatgcg
    20761 ccaacctgct ggtcaccccc cattgggcca ctcaacatcg cgttgacgtt catgccggtc
    20821 gcgcgggaaa ttaagttacc ggtagtgcct gttgcataca gtacgtgttg ttccagtaac
    20881 ggttgatgcc gttccaccca gctcatcagc atttgtttgc agtgatcgtg tgccaccagc
    20941 gcaatatgtt tccgcgcagg taaagtgcga gtcgtcagtt ccataatgta catccgtagt
    21001 taactttcct acagattact gtaagcactt atcgctgcaa gataaagacc gaaaaagcct
    21061 gcgcacaggc acaaaaatct caggaagatg gttgtttttc cgcccattgc aggaaagtat
    21121 ttcgcgtttg tgggtcagcc agtttaaacc aatacttcag ccgttgttct gtgagcacct
    21181 gagactgcgg tggaatagca tccagctcgg aaacccccga aagcagcgta ctgtcatctg
    21241 ccatcatcgt ggcaaattgc ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa
    21301 cctcgacctc ataatcaatc gtttttgccg ttgaggtaat ggcgagaata tccagcttta
    21361 ccggaatcgg cgtcgcatcg ccatccaaca attcaaggcg cggcgcagca tcaaaatggt
    21421 tagcttcccg ctcattctcc agacgaggca ggcgaaaatt gacctggttg atgagctggg
    21481 tattaaaact gaccaccagc ggtggggaga tatacagccg ttcttcacta ttggaaagat
    21541 gaatcgtctt ctcaacgcga aacactaact gatgcggtcc gttatccagt tcaatgctgt
    21601 ccgcaccgcg cagcagagag ctggaaacct ttttaccgtc gagtaccaga agatcgacat
    21661 cggttgaaag ccgcagcgtg gtggcaaaaa cggataccgg caaacataat gcaatcaagg
    21721 tggtcacgat gccggttttc ataggtagct cctgtcaaaa gaccgcccga ataataatgt
    21781 gtcaaaattt ttctttaatc atacttacta acatatagac atatttcccg cttttgctct
    21841 cattcattcg tattagctgc atggttggca tgtcggcttt gtcgtacact ttgttaaaca
    21901 gtctggagga aataatgaaa gaaaccgata ttgcaggcat tttaacgtcc actcacacca
    21961 ttgcgctggt gggggcaagc gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc
    22021 tactcgacca ggggtatcac gtcattccgg tttcgccaaa agttgctggc aaaacactgc
    22081 tgggccagca ggggtatggc acgctggcag aagttcctga aaaagtcgat atggtcgatg
    22141 ttttccggaa ttcagaagca gcgtggggcg tagcgcagga agcgatcgcc atcggcgcga
    22201 aaaccctctg gatgcaactg ggggtgatta atgagcaagc ggcagtactg gcacgggatg
    22261 ccggattaaa tgtagtgatg gatcgctgcc cggctattga gatccctcgc ctgggcctgg
    22321 ccaaataaaa aatccccgga aggcaaaaac cttccgggga tttgttcagg gattagttac
    22381 gcagacgcgg ggcctggagt tgtttgcgga tggtctgcgc cagctcatcc atggatggct
    22441 gttcaggatg ttcatcctgc aattcgctac ttagctgggc ttcagccagg taggtatgaa
    22501 ccggtaggcc gttatcgtcc tccatcacca cgtgatacca cggagcggcg cgaagctcgt
    22561 cattcaccgc cagctcatca ggcgacggtt cagagagcga ataaaccggg tcgatatcca
    22621 cgaccactcc gagataacct aacagggaat ggcggacctg ctggccgata ccgaatttgc
    22681 tggcaatcat agtcacctcc cgggaaatct ttacctttga tataagggta aaattccaca
    22741 tttcaagtta catgacgcga caggcaaacc ctttcagata tagcccttcc ggataggtag
    22801 cgatcaccgg atgatcggct gcctgacgga actgctctat aaattgtaca tcacgaccgg
    22861 catcaattgc ggcatccgcg atgattttct gaaataaatc gctggtcatc aggccagaac
    22921 aggagaaagt caggagaact ccgccttcat tcagcaactg aatcgccagc atgttgatgt
    22981 ctttatagcc acgacacgcg cccatcaact ggcttttatt ctcaacaaac ttcggcgggt
    23041 ccatcacgat aacgtcaaat ttttcaccgc gatcgcgata agtacgcagc aatttaaaga
    23101 catcatcacg gacaaactca gccttgctca gatccagttt gttcagctca acgttctgcc
    23161 gtgcaatatc cagcgcttcc tgggaggtat caacgctgac aacctggctg caaccgccca
    23221 tcagtgccga tacggcgaaa ccaccggtat aggagaaaca gttcagcaca cgtttatttt
    23281 caacgtagcg gcgggtagcc aggcggctgt cacgctggtc caggtagtag cccgttttgt
    23341 gcccgtgctg tatgtccacc agcagcttca tgccgtgttc ttcaatcggc agcagggcag
    23401 gcggtagctc tccggtaacg gggccctggg tcagttccat cccttctttt ttacgtaccg
    23461 caacgtcgct gcgatcgtaa atcgcacatt ccgggtacag cgtttgcagg gcactaatta
    23521 atgctgcgcg ctgatattct gcgccagcac tcagcaattg cagcaccaga aaattaccga
    23581 aacgatcgat agtaataccc ggcaggccat cagattcacc ggcgattaaa cgatagctgt
    23641 cgaggccatc tttttgcgcc agccagtcac gccatttttg tgcttgttgc aaacggcggg
    23701 aaaaaaaagc aatgtcgata gattcattcg ggtcaaacgt ccagacgcgc gcccggattt
    23761 gcgaagctgg cgaataagcg ccgcgtgcta accattttcc ctgatgatca acaatatcga
    23821 tggtttcccc gaggctggct ttaccttcca tgcgggcaac ggccccggaa aagatccacg
    23881 gatggcgacg aagtaatgat ttttcgcgcc ctttggctaa cactaaacgt acactcataa
    23941 tttacttttc tgtcgatgca aagaaatggg cgtcattgtc cggagttttc catggatttg
    24001 caacgcgtcc attaaggata aggagagcag caatgtcgaa agtctgcata attgcctggg
    24061 tttacgggcg agttcagggc gttggatttc gctataccac gcagtacgaa gcgaaaaaac
    24121 tgggattagc cgggtacgcc aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg
    24181 gtgaagaagg gcaggtagaa aaattaatgc agtggttaaa gagtggcggc ccgcgttctg
    24241 cgcgtgtaga acgggtgctc agcgagccgc atcatccctc ggaggaatta accgattttc
    24301 gtatccgtta aatacattta accggttttg gcaggccagc aattttggtg gcttgcttag
    24361 ccggaccttt cggaaacagt cggtataaat agcggctgtt acctttttct tcgccaaatt
    24421 tattcgccat cgcttttacc agcatacgaa tcgccggaga agtattgaat tccagataga
    24481 aatcacgcac aaaacgcacc acttcccagt gttctggcga cagcgaaatc ccttcgttct
    24541 ctgcaatcac cactgccagc ggctcactcc actggctgct ttctttgaga tagccttcgg
    24601 tatccgtttc tatctcttta ccttcgaaga tcagcataat tattactacc ttaatcagac
    24661 tggcggcagt gtaacaaaaa acaaagcccc gcataaagcg aggctcggta agtaaccctg
    24721 ggcgagatta atcgcggctg gcgaagccca gaatgctcag caggctgacg aagatgttgt
    24781 acagcgaaac atacaggcta accgtggcac gaatatagtt cgtttcaccg ccatgaatga
    24841 tgttgctggt ttcaaacaaa atagcgccag aggagatcag aatgaagacc gcgctgatcg
    24901 ccagatgcag agcaggcagt tgcaggaaga tattagcaac cataccaatc agcaccacca
    24961 caatacccgc catcagcata ccgccgagga acgacatatc tttgcgggtg gtcagcacat
    25021 atgcagagca gcagaagaac actaacgccg ttccgcccag tgccatagcg attacgtcac
    25081 ccattccggc agacagatag gtattcagaa ttggtccgag gatataaccc agaaaaccgg
    25141 taaaggcgaa tgcggagata atcccggtcg gcttattcgc cgttttatag gtcaggaaca
    25201 tcaaaccata catacccacc agcgtcagaa tcagacccgg agatggcagc atcagcaccg
    25261 tactggcagt tgcggtaatc gcagaaaagg ccagcgtcag gctcagcaga aaataggtat
    25321 tacgcagcac cttatgggtg ctaagcagcg atgtacggtc atgtgaagaa ctaacaatac
    25381 gatccattga gtcactctct atgacagatg taattaatta agcagcataa tgataatgcg
    25441 taagggcacc cagaagtttt acccatcttt acgcatttga tctggaacag gtttaacagc
    25501 ggattatcag gtcattaagc aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa
    25561 ggggttagca tttagctaac cccttatctt atttggcgga agcgcagaga ttcgaactct
    25621 ggaacccttt cgggtcgccg gttttcaaga ccggtgcctt caaccgctcg gccacacttc
    25681 cggaatgacg cgcactataa acatcccgat gcggcgtgta aacccctaat ttgtttgttt
    25741 gcctgaaaaa cagacaaaag tgcattgata gcgtgaaata acagcagatt gatcatttca
    25801 tcaccatgaa ttccttctct tttactcgtt tagcaaccgg ctaaacatcc ccactgcccg
    25861 gcaaaaagaa aaataggtcc atttttatcg ctaaaagata aatccataca gtttgtattg
    25921 ttttgtgcaa aagtttcact acgctttatt aacaatactt tctggcgacg tgcgccagtg
    25981 cagaaggatg agctttcgtt ttcagcatct cacgtgaagc gatggtttgc cttgctacag
    26041 ggacgtcgct tgccgaccat aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac
    26101 gtgcgatatg aataacgagg aaacatttta ccaggccatg cggcgtcagg gcgttacccg
    26161 gcgcagcttt ctcaaatatt gtagtctggc tgccacgtcg ctgggcttag gcgcgggaat
    26221 ggcaccaaag attgcctggg cgctggagaa caaaccgcgc attccggtgg tatggatcca
    26281 cggtctggaa tgcacctgct gtaccgaatc ttttatccgc tccgctcacc cgctggcgaa
    26341 ggacgtcatc ctttccctga tttccctcga ttatgacgat actttgatgg ctgccgccgg
    26401 aacccaggcg gaagaagtct ttgaagacat catcacgcaa tacaatggca aatatatcct
    26461 cgcagtagaa ggtaatccgc cgctgggcga gcaggggatg ttctgtatca gcagcggacg
    26521 accgtttatt gagaaactca aacgtgccgc tgccggagcc agtgcgatta tcgcctgggg
    26581 aacctgcgcg tcctggggct gcgtgcaggc cgcgcgaccc aatccgacgc aggcaacgcc
    26641 tatcgacaaa gtcatcaccg acaaacctat tatcaaagta cctggctgcc cgccgatccc
    26701 ggatgtgatg agcgccatca ttacttacat ggtgaccttt gatcgcttgc cagatgtcga
    26761 cagaatgggc cgtccactga tgttttatgg tcagcgtatc cacgataaat gttatcgccg
    26821 cgctcacttc gacgccggag agttcgtcca gagttgggat gatgacgctg cccgcaaagg
    26881 ttactgcctg tacaaaatgg gctgcaaagg gcctaccacc tataacgcct gttcctccac
    26941 acgctggaac gatggcgttt ccttcccgat ccagtcaggt cacggctgcc tgggttgtgc
    27001 ggaaaatggt ttctgggatc gcggttcgtt ctacagccgc gtggtcgata ttccacaaat
    27061 gggtactcat tccaccgccg acaccgtcgg tttaaccgcg cttggcgtgg tggcagcggc
    27121 tgttggtgtg cacgcagtcg ccagcgccgt tgaccagcgc agacgtcata accagcaacc
    27181 tacagaaacc gaacatcagc caggcaatga ggataaacag gcatgagcac tcagtacgaa
    27241 actcagggat acaccatcaa taatgccgga cgccgcctgg tggtcgaccc gattacgcgc
    27301 attgaaggcc acatgcgctg cgaagtgaat attaacgatc agaatgtgat caccaatgcc
    27361 gtctcctgcg gcaccatgtt tcgcgggctg gagatcatcc tgcaagggcg tgacccgcgc
    27421 gatgcgtggg cgttcgttga acgtatctgc ggcgtctgta ccggcgtaca cgctctggct
    27481 tcggtctatg ccatcgaaga tgctatcggc attaaagtgc cggacaacgc taatatcatc
    27541 cgcaacatta tgctggcgac gctctggtgc catgaccatc tggtgcactt ttatcagctt
    27601 gccgggatgg actggatcga tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc
    27661 gaactggcgc aaagtctctc ctcttggccg aaatcatccc ctggctattt cttcgacgta
    27721 caaaatcgcc tgaagaaatt cgttgaaggc ggccagttgg ggatcttccg caatggctac
    27781 tgggggcacc cgcagtacaa actgccgcca gaagccaacc tgatgggctt tgcccactat
    27841 ctcgaagctc tcgatttcca gcgtgaaatt gtcaaaatcc acgctgtctt tggcggtaaa
    27901 aacccgcatc caaactggat tgtcggcggg atgccttgcg ccattaacat tgacgaaagc
    27961 ggcgcggtcg gggcggtcaa tatggaacgc ctgaacctgg tgcagtcaat cattacccgc
    28021 acggcggatt tcattaacaa cgtgatgatc cccgacgcct tagccattgg tcagttcaac
    28081 aaaccgtgga gcgaaatcgg cacgggtctt tctgataaat gcgttctcag ttacggcgcg
    28141 ttcccggata tcgccaacga ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg
    28201 attaacggcg acttcaacaa tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag
    28261 gagtttgtcg accacgcctg gtatcgatat cccaacgatc aggtcgggcg tcatccgttc
    28321 gatggcatca ccgacccgtg gtacaacccc ggcgatgtca aaggcagcga taccaacatt
    28381 cagcagctga atgaacagga acgctactcg tggattaaag cgccacgctg gcgcggtaac
    28441 gcgatggaag tggggccgct ggcacgcacg ttaatcgctt atcacaaagg cgatgctgcg
    28501 accgttgagt cggtcgatcg catgatgtcg gcgttgaaac tgccgctttc cggcatccag
    28561 tcaacgttgg gccgcatttt gtgtcgcgcg cacgaagcgc agtgggccgc aggtaagttg
    28621 cagtatttct tcgataagct gatgaccaat ctgaaaaacg gcaatctcgc cactgcttcc
    28681 actgaaaaat gggaacctac aacctggccg acagagtgcc gtggcgtcgg ctttaccgaa
    28741 gcgccgcgcg gggcgttagg ccactgggct gccattcgcg atggcaagat tgatctctat
    28801 cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg atcctaaagg gcaaattggc
    28861 gcttatgaag cggcgctgat gaacaccaaa atggcgatcc ccgagcaacc gctggagatc
    28921 ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt caacacacgt gctgggcgac
    28981 gacggtagcg agctggtctc cgtgcaggtg cgttaacagc gaaggagaat catcatgcaa
    29041 cagaaaagcg acaacgttgt cagtcactat gtctttgaag cacccgtgcg gatatggcac
    29101 tggctgacgg tgttatgcat ggcggtgttg atggtcaccg gttactttat cggcaagccg
    29161 ctaccttccg tcagcggcga ggcgacgtat ctgttctata tgggctacat caggttaatt
    29221 cacttcagcg ccgggatgat ttttaccgtg gtcttgctga tgcggatcta ctgggctttt
    29281 gttggcaatc gttactcccg cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg
    29341 cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat
    29401 ataggccata accccatcgc ccaggcggcg atgttcggct atttcctgat gtcagttttt
    29461 atgatcatca ccggctttgc gctgtacagc gaacacagcc agtacgctat ttttgcgccg
    29521 ttccgttatg tggtggaatt tttctactgg acgggcggca actcaatgga cgttcacagc
    29581 tggcatcggc tggggatgtg gctgattggc gcgtttgtga tcggtcatgt ctacatggcg
    29641 ctgcgtgaag atatcatgtc cgacgacacg gtgatctcca ccatggtcaa cggctaccgt
    29701 agccacaaat ttggcaaaat aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg
    29761 taatggggct gggcaacctg ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac
    29821 ggctgtatgc ccattaccac tggcccgagt atgtggagat tgtcgatggc ggtactcagg
    29881 gactgaactt gctggggtat gtcgaaagcg ccagtcactt gttgatcctc gatgccattg
    29941 actacgggct ggaaccagga acgctgcgaa cctatgccgg agaacgcatt ccggcttatc
    30001 tcagcgcgaa gaaaataagc ctgcaccaga acagcttctc cgaagtgctg gcgctggcgg
    30061 atatacgtgg tcatttgcca gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg
    30121 acgactacgg cggtagcctg agcgaactgg cgcgggagca actgcccgct gcggaacaat
    30181 cggcgctggc gcagctggct gcgtggggaa ttgtgccgca accggctaat gaatcgcgct
    30241 gtctcaatta tgactgtctg tcgatggaaa attacgaagg cgttcgcttg cgtcagtaca
    30301 ggatgaggct ggaggaacag ggatgagcaa cgacacacca tttaatgcgt tgtggcaacg
    30361 aatgctggcg cgtggctgga cgccgatcag tgaatgccgt ctcgacgact ggcttacgca
    30421 agcgccagac ggcgtggtgt tattaagcag tgacccgaaa cgcacgccag aagtcagcga
    30481 taacccggtc atgattggcg aattactgcg cgagtttccc gactatacat ggcaggtagc
    30541 gattgctgac cttgagcaga gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc
    30601 cgccacatta gtatttaccg gcggaaacta tcgcggcgtg ctgaatggta ttcacccgtg
    30661 ggcggaactg ataaacctga tgcgcggact tgtcgaaccg cagcaggagc gtgcctcatg
    30721 agcgaaactt ttttccatct gctggggcca ggaacgcaac cgaacgatga cagtttcagc
    30781 atgaatccac tgccgatcac ctgtcaggtg aatgatgaac cgagtatggc agccctggag
    30841 caatgtgctc acagcccgca ggtgattgcg ctattaaacg agttacaaca tcaactaagc
    30901 gagcgccagc cgccgctggg cgaggtgctg gcagtcgatc tgttaaatct caacgccgac
    30961 gatcgtcact ttatcaatac gcttctcggg gaaggggaag tgtcagtgcg cattcagcag
    31021 gctgacgaca gtgaaagtga aatacaggag gcgatcttct gcggattatg gcgggtgcgc
    31081 agacgtcgcg gcgacaagtt gctggaggac aaactggagg ctggctgcgc gccgctggta
    31141 ttgtggcagg cggcaacgca aaacgtcttg ccgacagatt cgctgttacc gccgcccatt
    31201 gatggcctga tgaatggcct accgttggcg catgagttac tggcgcatgt acgtaacccc
    31261 gacgcgcagc cgcacagcat taatctgacg caattaccca tcagcgaggc tgatcggctt
    31321 tttctctcac gtctctgtgg gccgggaaat attcagattc gtaccattgg ctatggcgag
    31381 agctatatca acgccacggg gttacgccat gtctggcatt tacgctgtac ggacacctta
    31441 aaaggcccgt tactggaaag ttatgaaatc tgcccaatac cggaagtggt gctggcagcg
    31501 ccagaagatt tggtcgactc tgcgcagcgg cttagtgagg tatgtcagtg gctggcggaa
    31561 ggggcaccgt cgtaaaaaga cggtaagtat agctttcagg cttatgaata tcgcaatcgg
    31621 cgaatacctc tggtcgtaga gtttcaggat aaagagggag atctaccatt aacgggttat
    31681 ttttctctct tcgcctacag gagtgcgcat gtgggatgtc attgatttat cgcgctggca
    31741 gtttgctctg accgcgctgt atcacttttt atttgtaccc cttaccctgg ggctgatttt
    31801 tttgctggct atcatggaaa ccatttacgt ggtcaccggc aaaacaatct accgcgatat
    31861 gacgcgcttc tggggtaagc tcttcggtat caactttgct cttggcgtgg ctaccggcct
    31921 gacgatggag tttcagtttg gtactaactg gtcattctat tccaactatg tgggcgatat
    31981 ttttggcgca ccgctggcaa tggaagcatt aatggccttc ttcctcgagt ccacctttgt
    32041 cgggctgttc ttcttcggct ggcagcggct gaataaatac cagcacctgc tggtgacgtg
    32101 gctggtggcg ttcggttcaa atctctctgc gttgtggata ttgaatgcca acggttggat
    32161 gcaatacccg accggtgcgc attttgatat cgacaccctt cgtatggaaa tgaccagctt
    32221 cagcgagctg gtctttaacc cggtcagcca ggtgaaattt gtgcacaccg taatggcggg
    32281 ctacgtgacc ggggccatgt ttattatggc gatcagcgcc tggtatttac tgcgcggacg
    32341 ggagcgcgat gtcgcattac gctcgtttgc catcggttcc gtcttcggta ctctggcgat
    32401 tatcggtacg ctgcaactcg gagacagttc ggcgtatgaa gtcgcgcaag tacaaccggt
    32461 taaactggcg gcaatggaag gggagtggca aacggaacct gcacctgcac cgttccatgt
    32521 ggttgcctgg ccggaacagg atcaagagcg taacgccttt gccatcaaaa ttcccgcgct
    32581 gctagggatc ctcgccactc actcattaga taaacccgtg ccgggtctga agaatttgat
    32641 ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat
    32701 atcgcagggc aatcgtgagc cgcatgtgtt gcaggcattc cgggagctgg aaggcgacct
    32761 tggttacggc atgttgctct cccgctatgc gccggatatg aatcatgtca cagccgcaca
    32821 gtaccaggtg gcgatgcgtg gcgcgatacc tcaggttgcg ccggtattct ggagtttccg
    32881 catcatggtg ggctgtggtt ccctgctgct actggtgatg ctgattgcgc ttgtccagac
    32941 gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc
    33001 gctgccgtgg attgcgattg aagccgggtg gtttatgacc gagtttggtc gtcagccgtg
    33061 ggcgatacag gacatcttac cgacatactc cgcgcactcc gccttaacca caggacaact
    33121 ggctttctca ctgatcatga tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta
    33181 cctgatgcag aaatatgccc gtctggggcc gagcgcgatg cagagtgaac aaccgacgca
    33241 gcaacagggg taaaggagaa aatcatgttt gattatgaaa cattgcgctt catctggtgg
    33301 ctgctgattg gcgtgatcct ggtagtcttt atgatctccg atggatttga catggggatc
    33361 ggctgtctgc tgccgctggt ggcgcgtaat gatgatgaac gtcggatagt gataaacagc
    33421 gttggcgcgc actgggaagg caatcaggtc tggttaatcc tcgcaggtgg ggcattattt
    33481 gccgcctggc ccagagtgta tgcagcggcg ttttccggct tttatgtggc gatgatcctg
    33541 gtgctgtgct cgctgttctt ccgcccgctg gcctttgatt atcgcggaaa aattgccgat
    33601 gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg gcagtctggt gccgccggta
    33661 gtcttcggta ttgccttcgg caacttgctg ctcggcgtgc cgtttgcctt cacaccgcaa
    33721 ttacgcgtgg aatatctcgg cagcttttgg caactgctga cgccattccc tttattgtgc
    33781 ggattgctca gccttgggat ggtgattttg caaggtggcg tctggttaca actaaaaact
    33841 gttggtgcga ttcatctgcg ttcacagctg gcgaccaaac gcgccgcact gctggtgatg
    33901 ctctgctttt tgctggcggg ttactggctg tgggtcggta ttgatggctt tgtactgctc
    33961 gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac tggtggcagt gctacctggt
    34021 gcctggatga ataatttcgt cgaatcgccc gttctgtgga tcttcccgct gctggggttc
    34081 ttctgcccat tgctgacggt gatggcgatt tatcgtggtc gcccgggttg gggatttttg
    34141 atggcgtcat tgatgcaatt tggcgtgatt ttcacggcag gcatcacgct gttccccttt
    34201 gtcatgccgt caagcgtgag tccgatctcc agcctgacgt tgtgggacag tacttccagt
    34261 cagctgacgc tgagcatcat gttggtaatc gtgctgatat ttttgcccat tgtgttgctc
    34321 tacactctct ggagctacta caaaatgtgg gggcgcatga caacagaaac tctccgccgt
    34381 aacgaaaacg agctgtacta aggagcagga acaatgtggt atttactttg gttcgtcggc
    34441 attttgttga tgtgttcgct ctccaccctt gtgttggtat ggctggaccc gcgattgaaa
    34501 agttaacgaa cgtaagtctg atgcggcgca ttagcgtcgt atcaggcaat caataatgtc
    34561 ggatatgaaa agcggaaaca tatcgatgaa agcgatcttg atcccatttt tatctcttct
    34621 gattccgtta accccgcaat ctgcattcgc tcatagtgag ccggagctga agctggaaag
    34681 tgtggtgatt gtcagtcgtc atggtgtgcg tgcaccaacc aaggccacgc aactgatgca
    34741 ggatgtcacc ccagacgcat ggccaacctg gccggtaaaa ctgggttggc tgacaccgcg
    34801 cggtggtgag ctgatcgcct atctcggaca ttaccaacgc cagcgtctgg tagccgacgg
    34861 attgctgacg aaaaagggct gcccgcagcc tggtcaggtc gcgattattg ctgatgtcga
    34921 cgagcgtacc cgtaaaacag gcgaagcctt cgccgccggg ctggcacctg actgtgcaat
    34981 aaccgtacat acccaggcag atacgtccag tcccgatccg ttatttaatc ctctaaaaac
    35041 tggcgtttgc caactggata acgcgaatgt gactgacgcg atcctcagca gggcaggagg
    35101 gtcaattgct gactttaccg ggcatcggca aacggcgttt cgtgaactgg aacgggtgct
    35161 taatttcccg caatcaaact tgtgccttaa ccgtgagaaa caggacgaaa gctgttcatt
    35221 aacgcaggca ttaccatcgg aactcaaggt gagcgccgac aatgtctcat taaccggtgc
    35281 ggtaagcctc gcatcaatgc tgacggagat atttctcctg caacaggcac aaggaatgcc
    35341 ggagccgggg tggggaagga tcactgattc acaccagtgg aacaccttgc taagtttgca
    35401 taacgcgcaa ttttatttac tacaacgcac gccagaggtt gcccgcagtc gcgccacccc
    35461 gttattggat ttgatcatgg cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg
    35521 tgtgacatta cccacttcag tgctgtttat tgccggacac gatactaatc tggcaaatct
    35581 cggcggcgca ctggagctca actggacact tccaggtcag ccggataaca cgccgccagg
    35641 gggtgaactg gtgtttgaac gctggcgtcg gctcagcgat aacagccagt ggattcaggt
    35701 ttcgctggtc ttccagactt tacagcagat gcgtgataaa acgccgctgt cattaaatac
    35761 gccgcccgga gaggtgaaac tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat
    35821 gtgttcgttg gccggtttta cgcaaatcgt gaatgaagca cgcataccgg cgtgcgctct
    35881 ccatcaagac aaataaacat ttgccgctgt tggtcctcgc aggccattta cgcggcaaaa
    35941 ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg cggaaatatg
    36001 gacctggact tctttacgac cgtcggaggg gataatgaat cctttgccgc ttttgcgatc
    36061 aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact gaaaatactg
    36121 cgctgcacta tacggggtta ataaaataaa gccagcgata tttaagaccg ccggacggct
    36181 aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg tttgaaagac
    36241 agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta tccccttctt
    36301 ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa agccctgttt gccgaaaggc
    36361 ccaaaatgaa ggaagtaaaa tatgtctaat aaaattactg gtttagtaaa atggtttaac
    36421 gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt tttcgtccat
    36481 ttcaccgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa agttgaattt
    36541 tctattgagc aggggcaacg tggccctgcg gcagcgaacg ttgttacgct ctaaggttgc
    36601 cgttattact caacatctcc atttccgctg tccatgttgt catggttcac agtaccgcac
    36661 atcggcattt gatgtgacgg agcgaaatcc tttaggggca aagtgtattt tttgtaaatc
    36721 gacgatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg cgccgctgga
    36781 tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc ttatttttct
    36841 tcaacgctgt atgtatagta agcgataacc tgttgattat tgaatctttc ggggagatgg
    36901 cttataacat ttcttacctg accagggtac cgggaaccaa caccttactg gcgtgttgct
    36961 gtcttttaag accagaagag gttaacagtg aatattgaag agttaaaaaa acaagccgaa
    37021 acggaaatcg ctgactttat cgcgcaaaaa atcgccgagc tgaacaagaa tacagggaaa
    37081 gaagtctctg aaattcgctt caccgcacga gaaaaaatga ccgggcttga aagttatgat
    37141 gtcaaaatca aaataatgtg attttgtgaa catcaccccg tgcgaggtga tgttccgctt
    37201 gttgctaatt tagtgcccaa tcattggcgc ttgcggaatt aagcgtcggt acaattcctc
    37261 cggcaccggg ctttgccata ctcccgcata cattgcgtaa ccaatcaccg caaacataat
    37321 ccccagaacc agtagcgtca ttaaccagcc agacaacgca aaggcttttt tatttgctgc
    37381 aggtttttgc agtgaaaagg tcaatgttga ggctaccgga catgactcta cgcaactcat
    37441 acaaccggta cattccacgc ttcgtacctg aattaattta tcgaccggga tccgtgatgg
    37501 gcaatttttt gcgcatttgc cacagtcgat acaactttcg gcattgcgac gaatcttaaa
    37561 cggcgataat agcgaaacca cgcccatcag cgcgccatat gggcaaagat aacgacacca
    37621 ggcatggcga ataaacaggc tggcaatcag caaaacggtc acgctgatta atgtcgcggt
    37681 ccccatatga cgaaagaaat cgagcatttt aacgtccatc accacgctgt agggcgacaa
    37741 cataaaatag tgaatcgcct gagcgggcat caataacgca atatagagaa aaaaactcaa
    37801 cagcaaatac ttcacgccgc gcagaggaat atccagccag cggggaagga cacattgccg
    37861 accaaacagt ttgttaccga gatcgccgat taattcagaa agcgtaccaa ccgggcataa
    37921 ccatgaacaa aaggcctttt ttagtaatag actgatgacg ataaaagcga ccaataacag
    37981 catcgcggcg gcgtggacgg acggtaactg gcctgttgca aggctatatt tcagattcat
    38041 cagaccggca atcggtagcc agccttcgat gcccccgggc ctggcgacaa atgtcgtact
    38101 acctgtcgtt tcgtaatagc gcacccaata ccagaacgtg atggcaatat aaatattaat
    38161 tgccaacagc aataattgcg tcgctttacg ccaggtcgtg gcattgcgcc agtcattcca
    38221 cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag cgggttcttc ttttctctgt
    38281 catgattttg ccagtccgtt aagttgcata ccaaatgcca ctattctagt tgttcttaac
    38341 tggctgatat tgattcaaat cgcgttcagg tctttcttat ttaaccatgc tttcagagcg
    38401 gcaacactgc gtgtaatttc ttcgtgtgga aggggggcag ataatggctg ctgctccagt
    38461 tgtgcgcaaa gctggctggc gctacgcatt cccagactcg tgcaactgct ttttagctga
    38521 tgtgcggcac gctttatttt ttcgctgttc tggctggcgc gggcaatgtc gatttcatcg
    38581 agaagcggca gggcatgttg tttaaataat atcagccatt cgtggatctt ctccgtcccc
    38641 atcaactgag catcttcatt gagttgcgat acatccagcg gttgatcgtt attgacttgc
    38701 agttggagat agtgcgccag taactgaccg agcacttcac gcggcaccgg tttagggata
    38761 atcccgcgaa ataatgaact ggtacgctgg cgcaacgttt cgtcgatgac atgagcgcta
    38821 aagccaatca aaaccagcga cggatattgc tgtgccagtt gccgggcaag cgtaatgccg
    38881 tcgatatcgg gcagatcaaa atccaccagt gcggcagcaa acggttcgct attttgcaac
    38941 gtctctatcg cgtgtgcggc atttcctacc gcaacgacct gcgccccact ggtgttcagc
    39001 atctcgacgg taattcgttg ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg
    39061 tccagacgca ccgcctgatt gactgttttt ggcacgggag cagtggcaac acgtaacggc
    39121 aagcgtaaac aaaaacagct tccaacttcc ggcgtgctgg tggcgctcag ttcgccgccc
    39181 atcgcctggg ccagacggct actgatggtc agccccagcc cggtgccgcc gcgtttgccg
    39241 ctaacctgaa caaatggctg gaagatttca gccagtttcg tgggatcaat accgcagccg
    39301 ctgtcttcca cttcgaccag ccattgttcg ccatcagtgc gactacgcag gataatgtgc
    39361 ccttcgtcgg tgaaacgcag agcgttgctc aacaggttgg taatgacctg acgaatacgc
    39421 cgcggatcgc ccattaacgc agtcggtaca tcgtcggcaa ttgctgttgc caggtgaatc
    39481 gggcgacctt tcacccgtcc gctcattaat tgcagggtac tttccagcag cgggcgcggt
    39541 tcaaagggtt catcgctgac cgaaacattc ttgccgcctg cttcgatagc cgaataatcg
    39601 agaatatcgt tgaggatggt cagcaacgat tcaccagagt cagtaattgc gcgcaaatca
    39661 tcgcgctggg cgttaagtgc ggggttatct gccagcagct gtgcggtgcc gagaataccg
    39721 tacagcggtg tgcggatctc atggctcatc gccgccagaa acgccgattt tgcctggctg
    39781 gctttttctg cttccgcccg tgcctgtcgg tgttctatca ccagttcctg caattcagct
    39841 gtacgcgctt tgacctgtgc cgccagctgt tcgcggtggc gattcagtgc atgaacactg
    39901 ctgcgaaacg catccatcag ccgcccgatg gtgtccagct cccgcacgcc agcggtttcc
    39961 ggaaaagggg agtcgatatc accgtccagt aagcgttgca gcgcctgcgt ttgttcggca
    40021 agtggacgcg tgactgagcg ataaaccacg cgccagagga tcagaatcag tgcgcaaagt
    40081 gaaaccatcc ccagcaataa caggctgtac tgcccgcgtg cactggcttt ttccagatgc
    40141 gccagaccgt gctgattacg cagctcaatg gtgtcgacca gttgactgac ttcgctacta
    40201 aactgcgcga actgggcgat gttattttgc gtgagagttt gcaggcgatg actgatttca
    40261 ctgtcctgct gatacagcgc cagcaaatcg ctatattggc taacggaagt taacgttgtt
    40321 gcaacctgag cacgaactcc cggatcttca atgcgtattt gccgacgttg cagaattttc
    40381 accgcattat tgagctgctt ttccagcgtt ggcgcatttt tctggatctg ttccagcccc
    40441 agattcatca ccatttgctg cacccgcagg gcgctaaggc gcagttcatt catctggtta
    40501 acatactcaa gatcgatatc aatcagccga tcgagtgcac tttctgcggc ctgacgctga
    40561 tgttgttcga tcaaatcgta aatcccggcc tgggtcgctc cggcggaggt tgccgcatta
    40621 ttcgcctgac cttgcgccag gcgtgcgatc tcatcggcgg cggcgactat ctgctgactg
    40681 agtcgctgtt gttgctggcg cagttgcaaa cgctgcccca ccagttcccc ttgctgacgt
    40741 aacgaacggg agatctcctg ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc
    40801 cgtaacgctt gcagcaacgc attaatcttc aggctttgtg cggtgagcat tcgcccctgc
    40861 gcctgccaca tcttttcgtt atcggcactg gtcaggttct gcgcggcgaa aagttcccag
    40921 gcgctggctt cgctcaactg gcgcgccata ttcatggtag gaatcaatgc ctgagtgttg
    40981 tctttttcca cctggctgat aaagcgcagg ttgtaccatc ccaccagggt actggtcagg
    41041 gttaacagcg ccatcagggc aaagcccatc cagagtcttc gggtcagggt taaattcacg
    41101 gtcggtgcac ttaaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
    41161 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
    41221 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca ttacacggta
    41281 caagcctcta cgccgcttaa agccaaacgc gcatggaaac tgtgcgcgct ttatcccagc
    41341 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
    41401 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
    41461 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
    41521 tcatttcccg acctgcaaaa gcaggtagca agtctgccgg tgatcgaact ggtaaatgct
    41581 attgatgctc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
    41641 cctgggcgat atctggtgca atgggcgcac ggtaaaccac tgaacgtgct gttgatgccc
    41701 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
    41761 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgt
    41821 aacctgttgc aggagatgct ggagcgccat ccagaaatcg acgtcgttgc cggaacggcc
    41881 attgcggcag aggcggcaat gggggaaggg cgtaacctga aaacgccgct taccgtggta
    41941 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
    42001 gccagcgatc agatggtctg gcagggggaa ctggcggttg agcaggccat caggcaatta
    42061 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
    42121 gccgaccgtg aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
    42181 tatcagcaca catcagcggc taagaaataa ccttcgccat gttgcgtcac cagtaaatcc
    42241 gcgctgagtt tatggcgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
    42301 tccacccgac gcgcagagag catacgcagc agacgttcac ggctaagaat ttcgcccgga
    42361 ttcgtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
    42421 tcccgctcca gcgtatggcg cgacacattc aggcaataac cggcaaagcg atagcagttg
    42481 tcctgtgtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
    42541 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
    42601 agcccaacaa tacggtcaat ccgatcgctg cgtcccgtaa ccagaataat ccccaccgtt
    42661 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggcaa attaatatcc
    42721 agcagaatta aatccaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
    42781 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaaca
    42841 ggctcatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
    42901 tatctgttca tattctgccg taagccgttc atcctgacca gtgccgctgt tcatatttac
    42961 tcattaagat cgcttcacta aaccataatt ttacaggggc tattatgcgg aaactctgga
    43021 acgcgctacg ccgacccagt gctcgttggt cggtactggc gctggtcgct attgggattg
    43081 tgattggcat tgcgctgatt gtattgccac acgttgggat caaagtcacc agcacaaccg
    43141 aattttgtgt cagttgccac agtatgcaac cggtgtatga agaatataaa cagtcggtgc
    43201 atttccagaa cgcctccggc gtgcgagctg aatgccatga ctgtcatatc ccgccggata
    43261 ttccaggcat ggtgaagcgc aaactggaag cgagcaacga tatctaccag acctttattg
    43321 cccactctat tgatacacct gaaaaattcg aagccaaacg cgcggaactt gccgagcgtg
    43381 aatgggcgcg aatgaaagaa aacaactcgg caacctgccg ctcctgccat aactacgatg
    43441 cgatggatca tgcgaagcag catcctgaag cggcacgtca gatgaaggtg gcagcgaaag
    43501 ataatcaatc ctgcatcgac tgtcataaag gtattgccca ccagttgccg gatatgagta
    43561 gtggcttccg taagcagttc gatgagctgc gcgccagtgc taatgacagt ggcgacacac
    43621 tgtactccat tgatattaag ccgatttatg cggcgaaagg cgataaagaa gcctctggtt
    43681 ctctgctgcc tgcctcggaa gtgaaagtcc ttaaacgtga cggcgactgg ctgcaaattg
    43741 aaattacagg ctggacggaa agcgccggac gtcagcgtgt actcacccaa ttccctggca
    43801 aacgcatctt tgttgcctcg attcgtggcg atgttcagca gcaggtgaaa acgctggaga
    43861 aaaccaccgt tgccgacacc aataccgagt ggagcaagtt gcaggccact gcgtggatga
    43921 agaaaggcga catggtaaac gatatcaaac cgatctgggc ttacgcggat tcgctgtaca
    43981 acggcacctg taaccagtgc cacggcgcac cggaaatcgc ccactttgat gctaacggct
    44041 ggattggcac gctcaacggc atgattggct ttaccagcct cgataaacgt gaagaacgca
    44101 ccctgttgaa atatctgcaa atgaatgcgt ctgacaccgc aggtaaggct cacggcgata
    44161 agaaggaaga aaaataatga acaataacga tctctttcag gcatcacgtc ggcgttttct
    44221 ggcacaactc ggcggcttaa ccgtcgccgg gatgctgggg ccgtcattgt taacgccgcg
    44281 ccgtgcgact gcggcgcaag cggcgactga ggctgtcatc tcgaaagagg gcattcttac
    44341 cgggtcgcac tggggggcta tccgtgcgac ggtgaaggat ggtcgctttg tggcagcaaa
    44401 accgttcgaa ctggataaat atccgtcgaa aatgatcgcc ggattgccgg atcacgtaca
    44461 caacgcggcg cgtattcgtt acccgatggt acgcgtggac tggctgcgta agcgccatct
    44521 gagcgacacc tcccagcgcg gtgataaccg atttgtgcgc gtgagctggg atgaagccct
    44581 cgacatgttc tatgaagagc tggaacgtgt gcagaaaacg cacgggccga gtgccttgct
    44641 gaccgccagt ggttggcagt cgacggggat gttccataac gcttcgggga tgctggcgaa
    44701 agcgattgcc ttgcatggta atagcgttgg tacgggcgga gattactcta ccggtgctgc
    44761 gcaggtgatc ctgccgcgcg tagtcggttc gatggaagtg tatgaacagc aaacctcctg
    44821 gccgctggta ttgcagaaca gcaaaaccat tgtgctgtgg ggctccgatt tgctgaaaaa
    44881 tcagcaagcg aactggtggt gcccggatca cgatgtttat gaatattacg agcagctaaa
    44941 agcgaaagtc gccgccggtg aaattgaggt catcagcatc gatccggttg tcacatccac
    45001 ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc aaactgacgt
    45061 gccgctgcaa ctggctctgg cgcatacact gtacagtgaa aacctgtacg acaaaaactt
    45121 cctcgctaac tactgtgtag gttttgagca gttcctgccg tatctgctgg gtgagaaaga
    45181 cggtcagccg aaagatgtcg catgggctga aaaactgacc ggcattgatg ccgaaaccat
    45241 tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa attattgctg gctggtgcgt
    45301 tcagcgtatg cagcacggtg aacagtgggc gtggatgatt gtcgttctgg cggcgatgct
    45361 ggggcaaatt ggcctgccag gtggtggctt tggttttggc tggcactata acggcgcagg
    45421 cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt cgattccgcc
    45481 tgttcacgac aacagtgact acaaaggtta cagcagcacc attccgattg cccgttttgt
    45541 cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg taaaactgcc
    45601 gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc agcagatcaa
    45661 ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata accagtggac
    45721 ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg agcgtaacga
    45781 tctcgaccag tacggcaacc actctaaccg tggcattatc gccatgaaac aggtcgtgcc
    45841 gccgcagttc gaggcgcgca acgacttcga tattttccgc gagctgtgcc gtcgctttaa
    45901 tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac gcatctggca
    45961 ggaaggtgta caacagggca aaggacgcgg cgttcatctg ccagcgtttg atgacttctg
    46021 gaataacaaa gagtatgtcg agtttgacca tccgcagatg tttgttcgcc accaggcatt
    46081 ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg agatctactc
    46141 gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt ggtttgagaa
    46201 aatcgaacgc tcccacggtg ggcctggctc gcagaagtat ccgttgcatc tgcaatctgt
    46261 gcatccggat ttccgacttc actcgcagtt atgtgagtcg gaaactctgc gtcagcaata
    46321 tacggtagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg cgcgcggtat
    46381 tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg ctggggcagt
    46441 ggtttctgac cgctatgcac ccggcgtggc gcgaattcat gaaggggcat ggtacgatcc
    46501 agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg tgttgaccat
    46561 cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg cacactacgc tggtggaaat
    46621 tgaaaaatac agcgggacag tggagcaggt aacggcgttt aacggccctg ttgagatggt
    46681 ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct gacagcacaa
    46741 cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact ggacgatgaa
    46801 caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttctttgct gaaaagtgaa
    46861 ccgccgctcg cagcggcggt gagtgagctg gaaaaccgta ttgccactct gacagtacgt
    46921 gacgacgccc gtctggaact ggccgctgac ttttgcggtc tgtttctgat gaccgacaaa
    46981 caagcggcgc tgccttatgc atcggcctac aaacaggacg agcaagagat taaacgcttg
    47041 ttagttgagg cggggatgga aaccagcggc aatttcaacg aaccggcaga tcatctggcg
    47101 atctatctcg aattgctcag ccatctgcat ttttcgctgg gagaggggac cgttcctgcg
    47161 cgaagaatcg acagtttgcg gcaaaaaaca ctgacggcac tgtgggaatg gttaccagag
    47221 tttgctgcgc gttgccatca gtacgacagt ttcggttttt acgcggcact aagccagtta
    47281 ttgctggtgt tagtggaatg cgaccaccaa aacagataac gtcgtttgtt ggcctgataa
    47341 gacgcgttta gcgtcgcatc aggcatcagg gcgcagttgc cggatgcgtc atcacatccg
    47401 gcaaacacac atcacggatg agctacaaac cgggaaagcc gctggcgtag caggcggttt
    47461 tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg ccaccgcgat ccccggccag
    47521 tccagagcca gttcatgacg caggcgtact gcgcgttgca ccacgatggc ggcatggtcg
    47581 tcaaataccc aggttgtttc ctggatctca cgcggttcaa ccacccccaa accgacaatt
    47641 tcattcaact cctcttcaga gatgccggta tgcaggcaaa attcggtaat agtaaaagtc
    47701 accgtaacat tagccatcat gctttccccc aatctttacg tggatcaaaa gacgactggg
    47761 cgtctgccag ttgctgccac agcgcggcag tgttttcatc cggtttcggc ggcatcacga
    47821 ttttcagtac cgcatacaga tcgccggtct gttttttgct caccagacct ttgcctttaa
    47881 cgcgcaatcg ttgccctgcc tggctgccag gcgggatagt cagcaaaatg ctttctttca
    47941 gtgttggaac ggtgacttta gcacccagcg ccgcttccca cgggctaacc ggcaccacaa
    48001 tttccagatc ctggccgaca atatcaaaca gcggatgtgg cgcaatatga atcaccagcc
    48061 acaaatcgcc attcgggccg ccattttcgc caggcgtccc ctggcctttc aggcggatgc
    48121 gttgaccatt gccgacgccc gccgggatct tcacattcag cgttttcggg atttcctgtt
    48181 cgatcatgcc aaaggcgtta taaaccggca ggttatagct gatggtacgc ttatgctcag
    48241 taagcgtttc ttcgaggaat accgccactt cgatttcaat atcgtggccg cgtgtggcgg
    48301 ggcgttgacg gctctggcgg gcatgctgac cgaaaattga cgagaagata tcgtcaaaat
    48361 cttcggcgtt aaaactctga ccgtctccat gctggaactg acggctaaat tgcggatcgt
    48421 tgcgatgttg ccacatctga tcatactcag cgcgacgttg ttcatcactt aacacttccc
    48481 aggcttcagc gacctctttg aagcgggctt cggcatccgg ttctttgctg acatcaggat
    48541 ggtatttgcg ggccagtcga cgataggcgg tcttgattgt cttgagatcg tccgtcggtt
    48601 ttacgcccat gatggcgtaa taatccttta attccatagc gttatctcgc gtaaatcaac
    48661 acaaattgaa ggaacccctg taaggtaact tctataagtg tagggtaatc ctcaaaattt
    48721 catatgccaa cacagaatat gttattgaaa tcatcgcgga gaggaggtcg cctcaaggtg
    48781 gggtgctgaa catattttaa acaggtgaaa aagggtgagc gatttttgat agttgaacca
    48841 ggcactttaa gttttactag tgcgtcatta tttattaaat tttataggcg ctatatatgg
    48901 gtagtaatat acatggaatt agttgcactg caaataatta tttgaaacaa gcctggcaca
    48961 atataaaaaa agagcacgaa aaaaatcaaa catattcaat cacgcttttt gaaaacacac
    49021 tggtgtgttt tatgcggttg tataaagaaa tcagacgtca aaaagctgaa gactacattc
    49081 catgcctgga atgcgaatca ctagaaaaag aatttgagga aatgcagaat gataatgatc
    49141 tatcattatt tatgagaaca ttgcgtacta atgatacaaa aatttattca ggggtttcag
    49201 gaggtatgac atatactata caatatgttc aagatattga tgttgttaga gtgtccttgc
    49261 cgggcagagc ttcagagtct atcacagatt ttaaaggtta ttattggtat ggctttatgg
    49321 aatatattga aaacattaat gcgtgtgatg atgttttttc tgagtattgt ttagaagatg
    49381 aaaatataag tgtccagcca gaacggttaa atacgccggg aatatttaat ttagatgctg
    49441 gtattgattt gtctggtata tcttttattc agagtgaaat taataaaact tatgaattaa
    49501 aatacgctcc agtagatggc gatggatatt gtctgttaag
//
LOCUS       DARNVM010000013        48356 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.013, whole genome shotgun sequence.
ACCESSION   DARNVM010000013 DARNVM010000000
VERSION     DARNVM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.013"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            339..1556
                     /gene="alaA"
                     /locus_tag="SI621_000753"
     CDS             339..1556
                     /gene="alaA"
                     /locus_tag="SI621_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HEI4063506.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1640..2239
                     /gene="yfbR"
                     /locus_tag="SI621_000754"
     CDS             1640..2239
                     /gene="yfbR"
                     /locus_tag="SI621_000754"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HEI4063507.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2298..4130)
                     /locus_tag="SI621_000755"
     CDS             complement(2298..4130)
                     /locus_tag="SI621_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEI4063508.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4217..4867)
                     /gene="hxpA"
                     /locus_tag="SI621_000756"
     CDS             complement(4217..4867)
                     /gene="hxpA"
                     /locus_tag="SI621_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HEI4063509.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4878..5372)
                     /locus_tag="SI621_000757"
     CDS             complement(4878..5372)
                     /locus_tag="SI621_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEI4063510.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5455..5910)
                     /locus_tag="SI621_000758"
     CDS             complement(5455..5910)
                     /locus_tag="SI621_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HEI4063511.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6248..7450
                     /gene="ackA"
                     /locus_tag="SI621_000759"
     CDS             6248..7450
                     /gene="ackA"
                     /locus_tag="SI621_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEI4063512.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7525..9669
                     /gene="pta"
                     /locus_tag="SI621_000760"
     CDS             7525..9669
                     /gene="pta"
                     /locus_tag="SI621_000760"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEI4063513.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9859..11379
                     /gene="yfcC"
                     /locus_tag="SI621_000761"
     CDS             9859..11379
                     /gene="yfcC"
                     /locus_tag="SI621_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEI4063514.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11412..11954)
                     /gene="yfcD"
                     /locus_tag="SI621_000762"
     CDS             complement(11412..11954)
                     /gene="yfcD"
                     /locus_tag="SI621_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HEI4063515.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12012..12563)
                     /gene="yfcE"
                     /locus_tag="SI621_000763"
     CDS             complement(12012..12563)
                     /gene="yfcE"
                     /locus_tag="SI621_000763"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEI4063516.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12619..13263)
                     /gene="yfcF"
                     /locus_tag="SI621_000764"
     CDS             complement(12619..13263)
                     /gene="yfcF"
                     /locus_tag="SI621_000764"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEI4063517.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13399..14046
                     /gene="yfcG"
                     /locus_tag="SI621_000765"
     CDS             13399..14046
                     /gene="yfcG"
                     /locus_tag="SI621_000765"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HEI4063518.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14103..14465
                     /gene="folX"
                     /locus_tag="SI621_000766"
     CDS             14103..14465
                     /gene="folX"
                     /locus_tag="SI621_000766"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HEI4063519.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14486..15379
                     /locus_tag="SI621_000767"
     CDS             14486..15379
                     /locus_tag="SI621_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEI4063520.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15427..16317)
                     /gene="rpnB"
                     /locus_tag="SI621_000768"
     CDS             complement(15427..16317)
                     /gene="rpnB"
                     /locus_tag="SI621_000768"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HEI4063521.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLERGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16514..17287)
                     /gene="hisP"
                     /locus_tag="SI621_000769"
     CDS             complement(16514..17287)
                     /gene="hisP"
                     /locus_tag="SI621_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HEI4063522.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17295..18011)
                     /locus_tag="SI621_000770"
     CDS             complement(17295..18011)
                     /locus_tag="SI621_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063523.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVMALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18008..18694)
                     /gene="hisQ"
                     /locus_tag="SI621_000771"
     CDS             complement(18008..18694)
                     /gene="hisQ"
                     /locus_tag="SI621_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HEI4063524.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18784..19566)
                     /gene="hisJ"
                     /locus_tag="SI621_000772"
     CDS             complement(18784..19566)
                     /gene="hisJ"
                     /locus_tag="SI621_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HEI4063525.1"
                     /translation="MKKLVLSLSLVLAFSSATVAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19787..20569)
                     /gene="argT"
                     /locus_tag="SI621_000773"
     CDS             complement(19787..20569)
                     /gene="argT"
                     /locus_tag="SI621_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HEI4063526.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20835..21404)
                     /gene="ubiX"
                     /locus_tag="SI621_000774"
     CDS             complement(20835..21404)
                     /gene="ubiX"
                     /locus_tag="SI621_000774"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEI4063527.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADVETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21499..23016)
                     /gene="purF"
                     /locus_tag="SI621_000775"
     CDS             complement(21499..23016)
                     /gene="purF"
                     /locus_tag="SI621_000775"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEI4063528.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23053..23541)
                     /gene="cvpA"
                     /locus_tag="SI621_000776"
     CDS             complement(23053..23541)
                     /gene="cvpA"
                     /locus_tag="SI621_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HEI4063529.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23709..24371)
                     /gene="dedD"
                     /locus_tag="SI621_000777"
     CDS             complement(23709..24371)
                     /gene="dedD"
                     /locus_tag="SI621_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HEI4063530.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24361..25629)
                     /gene="folC"
                     /locus_tag="SI621_000778"
     CDS             complement(24361..25629)
                     /gene="folC"
                     /locus_tag="SI621_000778"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEI4063531.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25699..26613)
                     /gene="accD"
                     /locus_tag="SI621_000779"
     CDS             complement(25699..26613)
                     /gene="accD"
                     /locus_tag="SI621_000779"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEI4063532.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26769..27428)
                     /locus_tag="SI621_000780"
     CDS             complement(26769..27428)
                     /locus_tag="SI621_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4063533.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27511..28323)
                     /gene="truA"
                     /locus_tag="SI621_000781"
     CDS             complement(27511..28323)
                     /gene="truA"
                     /locus_tag="SI621_000781"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEI4063534.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28323..29336)
                     /locus_tag="SI621_000782"
     CDS             complement(28323..29336)
                     /locus_tag="SI621_000782"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEI4063535.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29402..30538)
                     /gene="pdxB"
                     /locus_tag="SI621_000783"
     CDS             complement(29402..30538)
                     /gene="pdxB"
                     /locus_tag="SI621_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HEI4063536.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAIPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQVGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30637..31632
                     /gene="flk"
                     /locus_tag="SI621_000784"
     CDS             30637..31632
                     /gene="flk"
                     /locus_tag="SI621_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HEI4063537.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDIQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVC"
     gene            complement(31629..32807)
                     /locus_tag="SI621_000785"
     CDS             complement(31629..32807)
                     /locus_tag="SI621_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063538.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFVLLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33072..34292)
                     /gene="fabB"
                     /locus_tag="SI621_000786"
     CDS             complement(33072..34292)
                     /gene="fabB"
                     /locus_tag="SI621_000786"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEI4063539.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34451..36457
                     /gene="mnmC"
                     /locus_tag="SI621_000787"
     CDS             34451..36457
                     /gene="mnmC"
                     /locus_tag="SI621_000787"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEI4063540.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAQWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQALPLPCATPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     KNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAIKAG"
     gene            complement(36513..36791)
                     /locus_tag="SI621_000788"
     CDS             complement(36513..36791)
                     /locus_tag="SI621_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HEI4063541.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36825..37373)
                     /gene="epmC"
                     /locus_tag="SI621_000789"
     CDS             complement(36825..37373)
                     /gene="epmC"
                     /locus_tag="SI621_000789"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HEI4063542.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLENGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37373..38182)
                     /locus_tag="SI621_000790"
     CDS             complement(37373..38182)
                     /locus_tag="SI621_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEI4063543.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38182..39006)
                     /gene="mepA"
                     /locus_tag="SI621_000791"
     CDS             complement(38182..39006)
                     /gene="mepA"
                     /locus_tag="SI621_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEI4063544.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGLGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39010..40095)
                     /gene="aroC"
                     /locus_tag="SI621_000792"
     CDS             complement(39010..40095)
                     /gene="aroC"
                     /locus_tag="SI621_000792"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEI4063545.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNVDVKTDIPRW"
     gene            complement(40130..41062)
                     /gene="prmB"
                     /locus_tag="SI621_000793"
     CDS             complement(40130..41062)
                     /gene="prmB"
                     /locus_tag="SI621_000793"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEI4063546.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41228..41779
                     /gene="smrB"
                     /locus_tag="SI621_000794"
     CDS             41228..41779
                     /gene="smrB"
                     /locus_tag="SI621_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HEI4063547.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(41849..42712)
                     /locus_tag="SI621_000795"
     CDS             complement(41849..42712)
                     /locus_tag="SI621_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HEI4063548.1"
                     /translation="MKIMRWILGLLISLFFSTAVQANVIVATMYTPIGYASYTTKITY
                     YLDVLTPDSVNHGVYETPNNTGLITGWIPLKSWTGPGPAPSLKVISMTAISQSSCPGL
                     AEYDSRAQRTMWTCYEIRMEVWKEDSSVHGCPWLVSTHADSIDLIDPAPGTYSGRTVS
                     NSSCPPIPLGPYDVSWNESRVVRDKTLALQSTGGIIEKTLSTYLMKDGKLCDGSKFGD
                     TDDRGAYCRWVSQMLTFTSSGCDNAKVTVTPNRHPITDKELHDMVLRVDTTSRQPIDS
                     TCRFQYVLNML"
     gene            complement(42714..43259)
                     /locus_tag="SI621_000796"
     CDS             complement(42714..43259)
                     /locus_tag="SI621_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4063549.1"
                     /translation="MNKLKKYQCIRVQTLLLGGVLVLASHSALAANNLHFYGNLMSKT
                     CTPVEQAGTLAEVQFADISYKDLMVRGQSDRVPVVFRLKDCRGPAQYSVKVTLTGTED
                     SGQPGFLALDDTSGAQGVGIGMETTDGTAVAINNTTGMTFVLNDGNNSLNFRAWLQAK
                     EGRDVTPGDFTAQLTATFEYI"
     gene            complement(43256..43735)
                     /locus_tag="SI621_000797"
     CDS             complement(43256..43735)
                     /locus_tag="SI621_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4063550.1"
                     /translation="MKRLSLILLSAFCTITHAAPEDITFTGTLIEPPVCTVSNGDDIE
                     IQFIDVIIDNIDGVNYRKDVPYQITCDPDITDDAWVMTLTWTGTQTNYNDAAIQTDVT
                     GLGIELQQNGQPFKLNTPLNVTPANLPKLKAAPVKASDATLTDGNFSAYATLQVDYQ"
     gene            complement(43732..44223)
                     /locus_tag="SI621_000798"
     CDS             complement(43732..44223)
                     /locus_tag="SI621_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4063551.1"
                     /translation="MKRLLLAGLGGVLLTGSALAGETKSVPLTLRVLVDPPPPCTVKG
                     SNVDFGDVIIKKINGTEYRKDAGYTLNCANSLSDDLQMQFKGTTTTINGETVLSTGIT
                     GFGIRIENAADNSLFAIGDNTWTPFSVSSQPQLKAVPVKQNGVTLTGAQFNASMTMVV
                     DYQ"
     gene            complement(44239..44988)
                     /locus_tag="SI621_000799"
     CDS             complement(44239..44988)
                     /locus_tag="SI621_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEI4063552.1"
                     /translation="MTKYLSAKTLTTVLSLLAATVSFSAQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKLPYLAQSWIEDEKGVKISSPLAVLPPVQRIEAMMNGQVKIQGLPDIH
                     KLPADRESMFYFNVREIPPKSNKSNTLQIALQTRIKLFWRPKALEKNTTQNPWQHKVT
                     LTHNGQEFTVNNPTPYYVIFSNASAQKNGNPAAGFSPIVMSPKTSAPLNVKMESVPVL
                     TYVNDYGARMPLFFKCTGNSCQVDEEQSRKG"
     gene            complement(45008..47647)
                     /locus_tag="SI621_000800"
     CDS             complement(45008..47647)
                     /locus_tag="SI621_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEI4063553.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNALADDTIQFDGRFLDLKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNQPLPDDYDIYWYATENDPNKSYACLSPELV
                     AQFGLKEDIAKNLQWIRDGQCLNTALLAGTEISGDLGQSALLVSVPQAYLEYTDSEWD
                     PPSRWDDGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNLRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALLGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDDDRARTGNDKEMY
                     TVTYNQNFTDARVSVYLNYSHHTYWDRQDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSNQVSLYHRIDDASHYTLSAGTS
                     ENHSSVDGYYSHDGTMAKVDLSANYHEGQYTSAGISLQGGATLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATK
                     SVVQATLTEGAIGYRKFAVISGEKAMAVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSSPVIPHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(47731..48297)
                     /locus_tag="SI621_000801"
     CDS             complement(47731..48297)
                     /locus_tag="SI621_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4063554.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMATTFTGNPYSTN
                     ADNYSLSNMDNGTEIPNVSLVIGDQHGTGYALGAEIKQPIVKDSSTGKGKPKQTLNFK
                     AWLVGETDAVTPTPAPFETLTTFQITYL"
BASE COUNT        11870 a        12978 c        12024 g        11484 t
ORIGIN      
        1 ggaaatcatc acgctgttaa actctccgta atgcggaaat atcatcatca cgattcatta
       61 gtgaattctt aaacgaggca ttttcaaaca attatcttac agataaaaaa accacactca
      121 caattaagaa tcagaacaat tcactacata acattaatta ttaagcatat acacctcatt
      181 attttgccat tattaagtta ttaacagcac aatcaagcct tcccctctgg caaaatctta
      241 ttctgcagac cttcaaaacg ccgtcctggg ggagtacatt gttctaagct gacttccacg
      301 gcagggagtg gcgataacag caaaaaaggt caagattcat gtcccccatt gaaaaatcca
      361 gcaaattaga gaatgtctgt tacgacatcc gtggtccggt gctgaaagaa gcaaaacgcc
      421 tggaagaaga aggtaacaag gtactgaaac tgaacatcgg caatcctgca ccattcggtt
      481 ttgacgcgcc agatgaaatc ctcgttgacg tgattcgcaa cctgcctacc gctcaagggt
      541 attgcgattc caaagggctt tactccgcgc gtaaagccat catgcagcac taccaggctc
      601 gtggcatgcg tgatgttacc gtggaagata tttacatcgg caatggtgta tcggagctta
      661 tcgttcaggc aatgcaggca ttgctgaaca gcggggacga aatgttggtt cctgcacctg
      721 attacccact atggaccgcg gcggtttcgc tctccagcgg taaagcggtg cattatcttt
      781 gcgatgaatc ctctgactgg ttcccggacc ttgatgatat tcgcgctaaa attacgcctc
      841 gtacgcgtgg gatcgttatt atcaacccaa ataacccaac cggcgcggta tattccaaag
      901 agcttttaat ggagatagtg gagattgcac gtcagcataa tctcattatc ttcgccgatg
      961 aaatttatga caaaattctc tacgacgacg ctgagcatca ctcaattgcg ccgctggcac
     1021 ctgatctgct gaccattacc tttaacggcc tgtcgaaaac ataccgcgtt gcaggcttcc
     1081 gtcaggggtg gatggtgttg aacgggccga aaaaacacgc caaaggctac atcgaaggtc
     1141 tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga
     1201 ctgcactagg cggttatcag agcatcagtg aatttattac tcccggcggg cgtctttatg
     1261 agcagcgtaa ccgcgcgtgg gaattgatca acgatattcc gggcgtttcc tgcgtgaaac
     1321 cgcgcggtgc gctgtacatg ttcccgaaaa tcgacgccaa acgctttaat attcacgacg
     1381 atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt tctgttggtg caagggacgg
     1441 cattcaactg gccgtggccg gatcacttcc gcattgtcac gctaccgcgt gtcgatgata
     1501 tcgagctgtc tttgagtaag ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt
     1561 aatttcactg ccggagattg catccggcag tgttatcccg ccacaatgac ctgatgatgt
     1621 catcatacat aaggtcacta tgaaacagag ccattttttt gcccatctct cccgcctgaa
     1681 actcattaac cgctggccgc tgatgcgcaa cgtgcggact gaaaatgtgt ccgaacacag
     1741 tttgcaggta gcgatggtcg cccatgcgct ggcagctatc aaaaatcgga aatttggcgg
     1801 taatgtcaac gccgaacgta tcgctttact ggcgatgtac cacgatgcct cagaagtgct
     1861 caccggcgat ctccctactc cggtgaaata cttcaattcg caaatcgctc aggagtacaa
     1921 ggcgattgaa aaaatcgctc agcaaaaact ggtcgatatg gttccggaag agctgcggga
     1981 tatctttgcg ccgttaattg acgagcatgc atatagcgat gaagaaaaat cgctggtgaa
     2041 acaggcagat gcactgtgtg catatctgaa atgtctggaa gaactcgcgg cgggaaataa
     2101 tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt gaagcgcgcc gcagccagga
     2161 gatggactac ttcatggaag tctttgttcc cagcttccat ctttcgctcg atgagattag
     2221 ccaggattca ccgctgtaag cagccggagt ctgcgtcgca tcaggcaata agcgccggat
     2281 gcgacatcag gctcttgtca aaacggaaac agcatcggga tcatcaccac acaaaccgcc
     2341 atcacgataa tggtgaacgg tacccccaac ttcacaaagt cactgaagct gtaatttccc
     2401 ggacctaaaa ccagtgtgtt aaccggtgaa gaaaccggcg tcataaaggc ggcggatgct
     2461 gccatcgcca cgaccatcgc aaatggataa ggcgacacac ccatcgtttt ggcagcagcc
     2521 agcgcaatcg gagccatcaa caccgccgtt gcggtattag agataaatag cccaataacc
     2581 gccgacaaga caaacaaaca acccagcatc atatgtggcc cgtaaccgcc gccaatgtcc
     2641 atcagccctt tcaccgccag cgcgacacct cccgttttct gtaatgccac agcaaacggc
     2701 atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg atttataggc gctttcagca
     2761 tcgatacagc ggaatttccc catcagcagg caggcgatga tagcggcaac aggattagga
     2821 atttcatcgg tcagcattaa cgccaccatc aacaccaggc aaaaaatggc atgaggtgcc
     2881 tgactgtgtg ctggcgatgc ttcactcact tcttctggta agttcagcgc cacaaagtca
     2941 cgcccttgtt ttgccagcat accgatcagt ttccagttac ccacaaccag gatgatatcg
     3001 cccagcagca gaggctcatc cgccagcgaa ccttccatcg cctcgccatt gcgctttagc
     3061 cccaccacat tcagtccgta gcgggtacga aaaccaattt cgcgcaccga tttaccaata
     3121 agttctgact caggaattaa tgaaatctct gccatgccca catcaagtgc ctggtcagaa
     3181 aaatactcgc cgcgcagtac catcggctcc agcaattgct cactacaaaa ttgccggaga
     3241 tcgacatcag ccgcagacat atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc
     3301 ccattaacgt tcacgataac gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta
     3361 gcgccataac gctcacgtaa tttgagatcg tccagccgtt gaccaatcat tggcgatccg
     3421 gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat attcacggat aagatcgcga
     3481 aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg tatccccttt cagcatgaaa
     3541 cgcatcacta acatatacaa aatacccagc accagcacga ccaggccaat aggtgttacg
     3601 ctaaagaaac taaaaccatg atagccttca cgcagcaatt cactgttgac taccaggttc
     3661 ggcggcgtcg ccaccagcgt catcatgccg cttatcagcc cggcaaaact taacggcatc
     3721 atcaggcgcg acggcgacgt ttgcatacgc atggcaacgc ttaacaccac gggaataaag
     3781 atagcgacaa cgccggttga actcataaac gcgccaagcc ccgcgacggt cagcatcaac
     3841 aaaaccaaca ttttgatttc actgttgccc gccactttga ccagccatgt ccccattacg
     3901 gtggcaacac cggtacggac caaaccatcg ccaataatga acaaggcggc aatcaggaca
     3961 acgttaggat cggaaaagcc ggaaaatact tcggggaccg tcagcgttcc gcttaatgca
     4021 aacgcgacaa taacaaacaa agcgaccgca tccatacgca ctctgcccgt cgcaaacaag
     4081 acgatggcaa cggccagtaa tgaaagaacc caaatcaatt caccgttcac aacttatcct
     4141 tgttaattga gggggatgac ttgattctgc cataaaaaag ccccgacgag acggggctaa
     4201 atcatgatca ggtgtttcac tgaataataa catcgccatt tggctgtttg gtcacagtaa
     4261 tttgctccag actgtggagg accaaatcga cctcattcag gcgcggggta tctgccggag
     4321 cgttaaccgc aatgacatga caacccgccg ccaggccaga aagcacgcca gcgggagcat
     4381 cttccaccac cacacactcc tgcggcgcaa gccccagcag ctgcgcgcct aacagatacg
     4441 catcaggttc tggttttccg cgcttcactc gctcagcggt tacaaacacc tctggtgcgg
     4501 gaagcccagc tactttatgg cgcgctcgcg ctaccggcat ggagccagaa gtcacaatgg
     4561 cccacggaat acctgcttta ttcaaatgat tgagtaaggc gattgccccc ggaagcgcgg
     4621 taataccctc ggtttccgtg gcctcgattt gctccagaca cgtaaactcg gcggcaatat
     4681 cagcctcgga tttgcccgcc ataaaatggc gcagagaggt gatcgcctgt ttaccgtgaa
     4741 tgaaagccag cacctcttcc ggcgctaacc catgacgtct ggcccagttg ctccacgccc
     4801 gctctaccgc aggcagggaa tccaccagcg ttccatcaag atcaaacaga aaacctttgc
     4861 accgcacgcg gacctcctca ggcgttgata atttgattaa tttcgttggc gctcaaatgg
     4921 tactgacgcg ggcaggcatg ccacacatta agcatgcgct gatatttttc ccacattggc
     4981 gtctgggcgt taaaaccgtg agttccagcg tcaaaatggg tatagcgccc ttccacatta
     5041 accataaagc ggacataacc gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag
     5101 gtgacacgac gttcatcgat ggattgctgg tcctgcaaat tagaccagga aacatgcaac
     5161 gcatgataca tctccataat gtcgatgata gtgcggcagg tttcttcttt cagctcgcca
     5221 aactcgcgat ccagttcacg catctgtaat ccataaccac gctcaatgat tgtttgcagg
     5281 cgacggtaac gttcagcatt tgccggatcg agcatagtca tcatcttgta ctggttagac
     5341 aaaataagac gttgcgcgtt ggtcatttcc attgttgact cctgtatcac tctactacgg
     5401 tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg taatcagggg tcaattacaa
     5461 atcatcaagg aaagttttat ccagttgttt gaaggcgcgc ttaagcgtgt cagctaatgc
     5521 ctggtaatca ggcttgcctt caacgggtgc caacacctgt ccagactcct gcaatttacc
     5581 gcgaacttca taaaaccagt tgaggatagc agggggtaat ggcgttacag aacgcttgcc
     5641 cagccaccac aatccctgca tgggtaaact taaggcgaac agcgcagtgg caactgccgg
     5701 cccaagctga ccgcccaggg caatctgcca gcagagagta aatacggcga tcggcggcat
     5761 aaaacggatc gcataacgcg tcatcttgat aacgcgattt tcgacaaaga ccggggcaag
     5821 gcgtttttcc agcggccacg tctttgagta atgctgtccc cggcgaaaca agctaaaaaa
     5881 attaacagaa cgattatccg gcgttgacat gcttcacctc aacttcacat ataaagattc
     5941 aaaaatttgt gcaaattcac aactcagcgg gacaacgttc aaaacatttt gtcttccata
     6001 cccactatca ggtatccttt agcagcctga aggcctaagt agtacatatt cattgagtcg
     6061 tcaaattcat atacattatg ccattggctg aaaattacgc aaaatggcat agactcaaga
     6121 tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat gttaatcata aatgtcggtg
     6181 tcatcatgcg ctacgctcta tggctccctg acgttttttt agccacgtat caattatagg
     6241 tacttccatg tcgagtaagt tagtactggt tctgaactgc ggtagttctt cactgaaatt
     6301 tgcaatcatc gatgcagtaa atggtgaaga gtacctttct ggtttagccg aatgtttcca
     6361 cctgcccgaa gcacgtatca aatggaaaat ggacggcaat aaacaggaag cggctttagg
     6421 tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt aatactattc tggcacaaaa
     6481 accagaactg tctgcgcagc tgactgctat cggtcaccgt atcgtacacg gcggtgaaaa
     6541 gtataccagc tccgtagtga tcgatgagtc tgttattcag ggtatcaaag atgcagcttc
     6601 ttttgcaccg ctgcacaacc cggctcacct gatcggtatc gaagaagctc tgaaatcttt
     6661 cccacagctg aaagacaaaa acgttgctgt atttgacacc gcgttccacc agactatgcc
     6721 ggaagagtct tacctctacg ccctgccgta caacctgtac aaagagcacg gcatccgtcg
     6781 ttacggcgcg cacggcacca gccacttcta tgtaacccag gaagcggcaa aaatgctgaa
     6841 caaaccggta gaagaactga acatcatcac ctgccacctg ggcaacggtg gttccgtttc
     6901 tgctatccgc aacggtaaat gcgttgacac ctctatgggc ctgaccccgc tggaaggtct
     6961 ggtcatgggt acccgttctg gtgatatcga tccggcgatc atcttccacc tgcacgacac
     7021 cctgggcatg agcgttgacg caatcaacaa actgctgacc aaagagtctg gcctgctggg
     7081 tctgaccgaa gtgaccagcg actgccgcta tgttgaagac aactacgcga cgaaagaaga
     7141 cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg aaatacatcg gtgcctacac
     7201 tgcgctgatg gatggtcgtc tggacgctgt tgtattcacc ggtggtatcg gtgaaaatgc
     7261 cgcgatggtt cgtgagctgt ctctgggcaa actgggcgtg ctgggctttg aagttgatca
     7321 tgaacgcaac ctggctgcac gtttcggcaa atctggtttc atcaacaaag aaggtacccg
     7381 tcctgcggtg gttatcccaa ccaacgaaga actggttatc gcgcaagacg cgagccgcct
     7441 gactgcctga tttcacaccg ccagctcagc tggcggtgct gttttgtaac ccgccaaatc
     7501 ggcggtaacg aaagaggata aaccgtgtcc cgtattatta tgctgatccc taccggaacc
     7561 agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg caatggaacg caaaggcgtt
     7621 cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg gtggcgatgc gcccgatcag
     7681 actacgacta tcgtgcgtgc taactcttcc accacgacgg ccgctgaacc gctgaaaatg
     7741 agctacgttg aaggtctgct ttccagcaat cagaaagatg tgctgatgga agagatcgtc
     7801 gcaaactacc acgctaacac caaagacgct gaagtcgttc tggttgaagg tctggtcccg
     7861 acacgtaagc accagtttgc ccagtctctg aactacgaaa tcgctaaaac gctgaatgcg
     7921 gaaatcgtct tcgttatgtc tcagggcact gacaccccgg aacagctgaa agagcgtatc
     7981 gaactgaccc gcaacagctt cggcggtgcc aaaaacacca acatcaccgg cgttatcgtt
     8041 aacaaactga acgcaccggt tgatgaacag ggtcgtactc gcccggatct gtccgagatt
     8101 ttcgacgact cttccaaagc taaagtaaac aatgttgatc cggcgaagct gcaagaatcc
     8161 agcccgctgc cggttctcgg cgctgtgccg tggagctttg acctgatcgc gactcgtgcg
     8221 atcgatatgg ctcgccacct gaatgcgacc atcatcaacg aaggcgacat caatactcgc
     8281 cgcgttaaat ccgtcacttt ctgcgcacgc agcattccac acatgctgga gcacttccgt
     8341 gccggttctc tgctggtgac ttccgcagac cgtcctgacg tgctggttgc cgcgtgcctg
     8401 gctgccatga acggcgtaga aatcggtgcc ctgctgctga ctggcggcta cgaaatggac
     8461 gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg gcctgccggt atttatggtg
     8521 aacaccaaca cctggcagac ttctctgagc ctgcagagct tcaacctgga agttccggtt
     8581 gacgatcacg agcgtatcga gaaagttcag gaatacgttg ctaactacat caacgctgac
     8641 tggatcgatt ctctgactgc cacttctgag cgcagccgtc gtctgtctcc gccagcgttc
     8701 cgttatcagc tgactgaact tgcgcgcaaa gcgggcaaac gtatcgttct gccggaaggt
     8761 gacgaaccgc gtaccgttaa ggcagccgct atctgtgctg aacgtggtat cgcaacttgc
     8821 gtactgctgg gtaatccggc agagatcaac cgtgttgcag cgtctcaggg tgtagaactg
     8881 ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg aaagctatgt tggtcgtctg
     8941 gtcgaactgc gtaagaacaa aggcatgacc gaaaccgttg cccgcgaaca gctggaagat
     9001 aacgtagttc tcggtacgct gatgctggaa caggatgaag ttgatggtct ggtttccggt
     9061 gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc agctgatcaa aactgcaccg
     9121 ggcagctccc tggtatcttc cgtgttcttc atgctgctgc cggaacaggt ttacgtttac
     9181 ggtgactgtg cgatcaaccc ggatccgact gcagaacagc tggcagaaat cgcgattcag
     9241 tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg ttgctatgct ctcctactcc
     9301 accgggactt ctggtgctgg tagcgacgta gaaaaagttc gcgaagcaac tcgtctggcg
     9361 caggaaaaac gtcctgacct gatgatcgac ggtccgctgc agtacgacgc tgcggtaatg
     9421 gctgacgttg cgaaatccaa agcgccgaac tctccggttg caggtcgcgc taccgtgttc
     9481 atcttcccgg atctgaacac cggtaacacc acctacaaag cagtacagcg ttctgccgat
     9541 ctgatctcca tcgggccgat gctgcagggt atgcgcaagc cggttaacga cctgtcccgt
     9601 ggcgcactgg ttgacgatat cgtctacacc atcgcgctga ctgcgattca gtctgcacag
     9661 cagcagtaat ctcgtcatca tccgcagctt tgcgctgcgg atatctgaac cggaaatagt
     9721 cactatttcc ggttttttat tctcttaatt tgcattaatc ctttctgatt atcttgctta
     9781 gctgcgctgc atcaatgaat tgcgccatct cactttgcat acttaccact ttgttttgtg
     9841 caagggaata tttgcgctat gtccgcaatc actgaatcta aaccaaccag aagatgggca
     9901 atgcccgata cgttggtgat tatctttttt gttgccattt taaccagtct tgccacctgg
     9961 gtagttccgg tgggcatgtt tgacagtcag gaagtgcagt atcaggttga tggtcaaaca
    10021 aaaacacgaa aagtcgtaga tccacactca tttcgcattc tgactaacga agcaggcgaa
    10081 cctgagtatc accgcgtaca gctgtttacg acgggcgatg aacgcccggg cctgatgaac
    10141 ttcccgtttg aagggttaac ctcaggatcg aaatacggga cagccgttgg catcatcatg
    10201 tttatgctgg tgattggcgg cgcgtttggc attgtgatgc gtacaggaac cattgataac
    10261 ggtatcctgg cgcttattcg ccatacccgc ggaaatgaaa ttctctttat tcctgcgctc
    10321 tttattctgt tttcacttgg cggcgcggta tttggtatgg gagaagaggc cgtcgccttt
    10381 gccattatca tcgcaccgct aatggtccgg ctaggctatg acagtattac caccgtcctg
    10441 gtgacctata tcgccacgca aatcggtttt gccagttcgt ggatgaaccc gttttgtgtg
    10501 gttgttgctc aggggattgc cggcgttccg gtgctttctg gctccgggtt gcgcatcgtg
    10561 gtgtgggtta tcgccactct gattggcctg atctttacca tggtgtacgc ctcacgagtg
    10621 aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc gcttctttcg tgaaaagcag
    10681 gcggatgttg aacaacgtcc gtttaccttt ggtgactggc tggtattgat tgtcctgacc
    10741 gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg cctggtttat tccagaaatt
    10801 gccagccagt tcttcaccat gggtctggtg attggcatca tcggcgttgt tttccgcctt
    10861 aacggcatga cggttaatac catggcttca tccttcaccg aaggggcgcg aatgatgatc
    10921 gcccctgccc tgctggtagg tttcgccaaa gggattttgc tgctggtcgg taatggtgaa
    10981 gcgggtgatg ccagcgtgtt aaataccatc ctcaacagca ttgccaatgc cattagcggt
    11041 ctggataacg cggtcgcggc ctggtttatg ttgctcttcc aggcggtatt taatttcttc
    11101 gtgacgtccg gttctggtca ggcggcgtta accatgccgt tactggcacc gcttggcgat
    11161 ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc agtttggtga tggcttcagc
    11221 cacatcattt acccaacctc agcttcgtta atggcgacgc tcggtgtgtg ccgggtggac
    11281 ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac tgctgtttat tatgtccagc
    11341 gtcgtagtga tcggcgctca gttgatgggc taccactaaa aatgttaaga gccgcaattg
    11401 cggctctttt ttcattctgc cgtttcagtc tctacggctt catttttggc attgcgcttc
    11461 atccacaacg ccagcgcttt cagcgagtct ggagtgaact catcgcagcg tgcggtgatt
    11521 tcttccggtg tcagccagca aacttcactg acttcatctt cctgtagggc gaagggaccg
    11581 tgagagacgc aactgaacaa cgcgccccag acacggcaat ttttatcttc gaaatagaac
    11641 tgcccgtgct cggcaaaggg gacaccggca atgcccaact cttcttccgc ttcgcgacgc
    11701 gcggattcca gcagttgctc atcggcctgg actacaccgc ctgcggtcgc atctaacatg
    11761 ccgggtaaaa agtcttttgt ctcggtacga cgctggacca gaattttgcc catgccatca
    11821 tgcacgacga tgtaagttgc acgatgacgc agacactgtg cccgcatttg ttcccggctg
    11881 gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca cccattcagt acttgccaaa
    11941 cgacgctgtt ccattatcag gaaaccttct ttttctggcg ctcttacggc gcattttgag
    12001 ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga tactttgatc attgagtgcg
    12061 attacactaa gtacgtcatt atccagcatg ccataactcg ccggattacc gcctttcgga
    12121 atactcaccg agccggggtt gaagtggaaa atctcccccc gctgttctgc cactggcaga
    12181 tgggtatgac cgtacaccag cacatcgttc tggtttaaag caggcagatt ttccggccca
    12241 aacagatgac catgcgtcaa aaacagacgt tgtttttcca gtaatacctg ttgccacggc
    12301 gcggttatcg ggaaatgcaa aagcatttga tccacttcgc tgtcgcagtt gccgcgcaca
    12361 gcgataacct tatgtgccac ttcattaagc cgttcagcga ctttggctgg cgcgtaaccc
    12421 tccggtaaag cattacgcgg gccgtgattc aacacgtcgc caaggatcac cagccactgg
    12481 gcaccgcttt gggcaaacaa ctccagaaca cgttccgtcg ccggtaacga cccatgaatg
    12541 tccgatgcaa acatcagttt catcactcac tcctcgtcga aaaaacgtaa gctatgatac
    12601 tggattctgt cgttgctatc agccagattg cttcgccgaa agtgcaataa aacgctggac
    12661 agacgctcgc tgccactgga atgtcgcata atccaccagg cgttccggca cctcatcgcc
    12721 atggagtacc aggcggttaa tcattagcgc cagatcagta tcagcaatgc accattcacc
    12781 aaataaattc ggctgaccca gtgctaacaa atgttctgcc atcgcgaaca gtttctctgc
    12841 actggctttt ccctccgccg ttaatggcgc ttttttcgca cccgcaaaga caacatccgt
    12901 tggacgttcc tcgcggatgg gcatcagatc gctacgcagc caggcctgaa tctgtcgtgc
    12961 acgcgcacga ttttctaaat caagcggata aatgcgttcc caggtcggtg gcgcaaatcg
    13021 atcttccaga tactccgcaa tggcagaaga ttcactcaac tcaaaatcat caatttgtaa
    13081 taacggcaca cggcgtgtct gaccgtaacc ttgccacgtc ggctgcaaat gttcaccgct
    13141 gtcgaggtcg atggtcttga tatgaaacga caggcctttt tcctgcaacg ccacccaggc
    13201 ggataacaca taaggggaga aaaaatgggc atctgaccaa agcgtgatag cgggtttact
    13261 cattatgtcc tcggcaataa agggggctgg ctatcaaact atagagcctt tttgtcactg
    13321 tcacgtgacg aatactcacg caagtcgccg cgctttctat acttgtggtg ttcagatgat
    13381 tagacaaccg gagctgcaat gatcgatctc tatttcgccc cgacaccaaa tggtcacaaa
    13441 attacgctgt ttctcgaaga agcagggctg gattatcgct tgattaaggt agacctgggg
    13501 aaaggcggtc agtttcgccc ggaatttttg cacatttcgc ctaacaacaa aattccggca
    13561 attgttgatc attctccagc cgatggcggc gaaccgctaa gcctctttga gtctggtgcc
    13621 attttgttgt atctggctga gaaaacagga ctctttttga gtcatgaaac gcgtgagcgc
    13681 gccgccacat tacagtggtt attctggcag gtaggcggac tggggccgat gcttgggcag
    13741 aatcatcatt ttaatcacgc tgccccccaa accattcctt acgctattga acgttatcag
    13801 gttgaaactc agcgtctgta ccatgtactg aacaagcggc tggaaaactc gccctggctg
    13861 ggaggtgaga actacagcat tgcggatatt gcctgctggc cgtgggttaa tgcctggact
    13921 cgccagcgaa ttgaccttgc aatgtatccg gcagtcaaga actggcatga gcggatccgt
    13981 tcgcgccctg ccaccggtca ggcactgcta aaagcacaac tcggtgatga gcgttcggat
    14041 agttaacaga aacaggttct cgtgtattat ttcattctaa gtaaaacaac ggagaacctg
    14101 caatggcaca acctgccgct attattcgta taaagaacct tcgtttacgt acgtttatcg
    14161 gaattaagga agaagaaatt aacaaccgtc aggatattgt tatcaatgtg acgatccact
    14221 accccgccga taaagcgcgc actagcgaag atatcaacga tgcgctgaat tatcgcaccg
    14281 taacgaaaaa cattattcag catgtagaga ataaccgttt ctctttgctg gaaaaattaa
    14341 ctcaggatgt gctcgatatc gcacgtgaac atcactgggt gacgtatgct gaagtggaga
    14401 tcgataaact gcacgcgctg cgctacgctg attcggtatc catgacctta agctggcagc
    14461 gttaaccgcc atatcgggag gctgcatgaa catagtgatc accggaggga cgggattaat
    14521 tggtcgccat ttgattccac gtttgctgga gctgggccat caaatcacgg taatgacgcg
    14581 taacccgcag aaagccagtt ccgttctcgg ccctcgggtg acactatggc aagggcttgc
    14641 cgatcaaagc aacctcaacg gcgttgatgc ggtaatcaat ctggccggag aaccgattgc
    14701 tgataaacgc tggactcacg agcaaaaaga gcgtctctgc caaagccgct ggaatatcac
    14761 gcaaaaactg gtcgatttga ttaatgccag cgacacgcca ccgtcggtac tcatttccgg
    14821 ctcggcaacg ggctattatg gcgacttagg tgaagtggtg gttaccgaag aggaaccgcc
    14881 gcataacgaa tttacccata aactctgcgc ccgctgggaa gaaattgcct gccgggcaca
    14941 aagtgacaaa acgcgagtgt gcctgctgcg taccggcgta gtgttggcac cggatggcgg
    15001 tattctcggt aaaatgctgc cgccgtttcg tcttggtctg ggcgggccga ttggctccgg
    15061 tcggcagtat ctggcctgga ttcatattga tgatatggtc aacggcattc tctggctgct
    15121 ggataacgag ctgcgtggac catttaatat ggtttcgccc tacccggtac gcaatgaaca
    15181 atttgcccat gcgctcggtc atgcgctgca tcgcccggcc attttgcgcg tccccgcgac
    15241 cgccattcga ctgttaatgg gcgaatcttc agtactggta ttaggtggac aacgcgcgct
    15301 gcctaaacgg ctggaagaag cgggttttgc gtttcgctgg tacgatttag aagaggcgct
    15361 ggcggatgtt gttcgctgat gtggtttact gcaaacatcc gccagttaac tcctggtgtt
    15421 acagaattag tggctttgcg cgataagatc gtctggtgaa agtcgggtca ccatcataac
    15481 taactctctg tctaaacctc tctccagcat ctcctgagca atacgcaggg cttcttcgtg
    15541 tttgccctgc attgcgcctt cttcacgtaa tctgtcagca atagtcatca gtttctcctt
    15601 ttcttgtggt gcgcgttccg ctatctcacc aataaatgca cggaaacgct gggcatctcc
    15661 cgtttgtaat acataattaa acagggcttt tagctgcctg tcattagtgt ttcctgtaac
    15721 tagcagcgaa acaatttggt cgactaatcc caacagatcg cgctgacgaa tatgtttctg
    15781 aattaactcc aacagcgcca ttttgcgatg ttgcataatc tcgtcatccg gcaccacggt
    15841 aatatccacc agcggaaaag ccgatgaata tattttgcgg gctatagcag gctcggcaaa
    15901 ttcatcaagc cagcagagtg aataaggata aggacttctg caaccatgat aaaacagcat
    15961 cgggatcacc aatggaagct ctttatagcc tgcatcaaga tggttttgca tcgccgcaat
    16021 ggaatagcgc atcatgcgaa aagccattaa ttcttccggc ttactttggt gctctatcac
    16081 tacataaata taacccgctc cctcctgcgt tttcacagac cacaagaggt cggaataata
    16141 ttgccgcagg tcttcatcaa taaaactgtt tggttccagt ttaagcgttg ttaaatcaca
    16201 cagtttgcgc agcggcgcgg gaagatgaat atcaataaaa tcccgcgcgg tgtctggatg
    16261 gcgtaaaaaa gatttaaata ccgcatcatg cggcgtggaa gttgtcgata ttgtcatggc
    16321 gttccgtcac ccctcaaatt taagataacg cgacaataac cacgattaac acccaatgca
    16381 gcaggctgtt tttatctttg gacagcgaat tcagaactat ttttagcgga ctgcaacttt
    16441 tacatttcag cggaagctga ctcgcaaatt ccccttgaaa atactccggc agcgttagcc
    16501 accggagggg gaattatttc agcgatccct taaggaactg ttgcagacga gggctttgcg
    16561 ggttaccaaa taactgttct ggcgcaccct cttcttctat tttcccctga tggaggaaaa
    16621 tgacgtgagt agaaacatgg cgagcaaagc ccatttcgtg agtcactacc accatggttt
    16681 tcccctcttc tgccagttgc tgcataatac gtaacacctc gcctaccagt tcaggatcga
    16741 gcgccgaggt aggttcatca aacagtaaaa cttccggttc cattgccagc gcccttgcga
    16801 tggaaacacg ctgttgctga ccaccggaca gatgcaccgg gtatttcccc tgcgcacgtt
    16861 cgtctatccc aacttttgcc agatacttca ccgcccgctc gcgcgcttcc tgcttgctca
    16921 ggcccaacac ctgaatcggc gcttccatga cgttttccag caccgtcata tggctccaga
    16981 gattgaagtg ctggaatacc atcgtcaggc gtgtgcgcag taagcgcagc tgatttttat
    17041 cggcgacttt gagttgaccg tctttgtcgc gcaccagatt gatcgtctgg ccattgacca
    17101 cgatcgatcc ttcactcggt ttttcgagga agttaatgca gcgcagaaag gtacttttcc
    17161 ccgatcccga cgatccgatg atgcttatga catctccggc attcgcttgc agtgagaccc
    17221 ctttcagcac ttcgtgttcg ccgtagcgtt tgtgcaaatc gataacgttt aatttattct
    17281 cggacatcgt attctcagtg cgttgaagaa ggtttcacat gctgcaacca gcgtttttcc
    17341 gctctgcgaa agaggctgat caggacataa gagatgatta aatagagcac cgcggcaatg
    17401 ccgaaggcgg taaaaggttg atacgtggcg gcgttaatat cgcgggctat tttcagcaga
    17461 tccggcacag tagcggtgaa agccagcgca gtagagtgca gcatcaggat cacttcgttg
    17521 ctgtaggccg gtaacgcaat acgcagcgcc gatggcaaaa taatgcagcg atacatttta
    17581 aaggtcgaga agccataggc tctggcggct tcgatttccc catgcggcac tgaacggatc
    17641 gctccggcaa aaatctcggt agtgtaagcg caggtgttga gcgtcagcgc cagcacggta
    17701 cagttcaggc cactgcgaaa aaaggcgtta aggaactcgg ttcccttaac aatttccagc
    17761 gtgtacatgc cggaatagaa caccagcaac tgaacataca gcggcgtacc gcgaaaaata
    17821 tacgtaaata accagattgg aaactggatg tatttattac ttgagacgcg accaatcgcc
    17881 agaaacagcg ccatgactcc gcctatcact accgacaaaa tcagcagcca cagggtgatc
    17941 gccacgccag taaagcgata accgtcagtc cacagcagcg gtttccagta ttcatgtaag
    18001 atttcgatca caggtcagcc ctcttcacac ccacggagta gcggcgctca aggaacagca
    18061 gcacaccatt ggaaacagtg gtgaaaacca ggtaaatcac gccacagacg atggcgaaat
    18121 agaacggttc ccaggtactt ttgcctgcca gctgcgtggc tttgaccaca tcttccaggc
    18181 cgagtaacga aacgagtgcg gtagatttga ggatcacctg ccagttgttg ccaatgcctg
    18241 gcagggcgta acgcatcatc gccggaaaca tgatccgccg aaacacttgc ccacgagtga
    18301 aaccgaacgc cgtcgccgcc tctatatgtc ctttcggcac tgccataaaa gcgccacgga
    18361 acgtttcggt aaaataagca ccgtagataa aaccgagagt aataatacca gcgaccatcg
    18421 gatcgatatc aatctgcccg acgcccatcg cctccgtcac cgtgtttagt gcaatctgca
    18481 aaccgtagaa aattagcagc atcaacacca agtccggcac gcctcgaatc agcgtggtgt
    18541 acccttcgaa aatcagcccc gaaagccgat tttgcgagag cttaccgcca gcgccaatta
    18601 aaccgatgat tacagcgagc actacagagc tgatagccag ctccagcgtg acgagcgcac
    18661 cctgtaaaat aacacctgaa aacccataca acatgctgcc tgtcctgtcg tgtgtggtga
    18721 attaccgcct cttgtctccc tccggttacc cgaagggagc ggactttctt tcaccgatgg
    18781 cgattaacca ccataaacat caaaatcgaa gtactttttc gctaatttct cgtaagtacc
    18841 gtcagcgcgc atttcggcaa aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt
    18901 acgcaggccc atgccggtcc ctacgccaaa cagtttttca tctttaacag acgggccacc
    18961 gaatttgtaa tctttaccga cgggttgttt gaggaaacct tcgctggcag cgacctcatc
    19021 ctggaacgcg gcatcaatac gtccggcagt caggtcagaa taaatattgt cctgcccttg
    19081 ataagaaacg atttcaatgc cttttggtgc ccagtgttcg ttaccgaacg tctcctgggt
    19141 ggtgccctgc aatacgccta cccgtttgcc tttcagcgac tcgactgtcg gctgaatgtc
    19201 ggaatttttc gccaccacca gacgagaatc ggcagcgtac agtttgtcgg tgaaggctat
    19261 ttcttgctga cgtttttccg taatggaaag cgatgacatg atggcgtcaa tcttcttcgc
    19321 ttttaaggac gggattaacg catccagtgg gttttcgaca aacgtacatt gcgtattgat
    19381 gcgtttgcac aactccttcg ccagatcgat atcgaagcca accagttcgc cttgtgaatt
    19441 ttttgattca aatggcgcat aggtcgggtc ggtaccgatg cggatgtttt gcggaatcgc
    19501 agcaaacgcc acagttgcgc tggagaaggc cagaaccaga gagagcgata gcaccagttt
    19561 tttcataatg tcctcaactg acagtctttt tatagggaat atttacaggt ttgttacact
    19621 tatcgtgcca taaaattgac catcaaggca aaatatcccg ccccgttaga cgtattattt
    19681 ttcattaaaa atgcttaagt atgcatttca atgcaccacg atagtgcaac aaacctggcg
    19741 tgcaccatga tgatcattcc atcaggtaca gcttccctgc gacgtatcag tcaccgtaga
    19801 cattaaagtc aaaatacttt ttcgccatct tgtcgtaggt gccgtcctga cgcagctcac
    19861 caagcgcctt attgaaggca gccgtcagtt cagcatcatc tttacgtagc cctacaccgg
    19921 tgccgtcacc gaagtatttt ttgtctttca ctgatgagcc agcaaaggcg aagtctttac
    19981 cagcaggttg cttgaggaat ccttcgctgg cagcaacttc atcttgtaac gcagcatcca
    20041 gacgtcctgc agccagatcg gaatagacca aatcctggtt ggcataggcc accacatcca
    20101 cacctttgct acgccaggtc tcgttagcgt aagcttcctg ggttgatccc tgcagcacac
    20161 caacatgttt acctttcagt gaatccagcg ttggctgaat cggcgaacct ttggcggcaa
    20221 tcaaacgaga atctgcggcg tacagcttgt cggagaaagc aatctcctgc tgacgtttat
    20281 cggtaatgga aagcgacgaa ataatggcgt cgattttttt cgctttcagt gaggggatca
    20341 gcgcgtcaaa gtcactggca acccaggtac atttcacctg catccgtttg cacatctcgt
    20401 taccgagatc gatatcaaag ccaacaaaat cacctttagc atctttcgat gagaacggcg
    20461 cataggtggt atcagttccg atacgtaccg tctccggtag cgccgcatag ctggaagccg
    20521 ctgcggagag accgactaac aaagacagag cgagaatcga cttcttcata cataaccctc
    20581 aagtgaaaat ggctttttta tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc
    20641 aggtcttatg ccatcttgac gacaacagta acattcaacg ttaaatatgt taataagacg
    20701 ttgcattatt gtcctgaagt tgaagatagc aggtatagcg gttgaatcgc agcgtttcga
    20761 ttgaatggca gaaacaaaat gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca
    20821 gggcaacagc ggagttatgc gccctgccag cgggcaaaga gatcttcagg aagcgttatg
    20881 gcgaattgat cgagtacacg gttaaccgtc tgatttatca catcatcaag ggattgcggg
    20941 cgatgataaa acgccggaac gggaggcata atcaccgcac cgatttctgc tgcctgagtc
    21001 attaaacgca gatggcctaa gtgcaatggt gtttcacgca cgcagagcac cagcgggcga
    21061 cgctctttca gcacaacatc tgccgcacgg gtcagtaagc catcagtata gctatggaca
    21121 atgccggaca gggttttgat cgaacagggt aaaatcacca tccccagcgt ctggaaagaa
    21181 ccagaagaga tgctggcagc aatatcgcgc gcatcgtgcg tcacatcagc taacgcctgc
    21241 acttcgcgca gggaaaaatc cgtttcaaga gataaggtct ggcgcgcggc ctggctcatt
    21301 accagatgcg tttcaacatc tgcgacatcg cgcagaacct gtaataagcg cacgccataa
    21361 atcgcgccgc tggcaccgct gatgcccaca ataagtcgtt tcataaatag ttgccctgtc
    21421 agacttgcag gcagactttg caggatttcg gcgggagttg caagtcaggg tgccagaccg
    21481 gcaccctgag cgaaggcatc atccttcgtt atgcatttcg agattttcca cttcgttctg
    21541 acgttgcacg gctttggcgt catcattacg taaagtatca aggaaatcga ggtagccctg
    21601 atcaacatct ttggtgacgt agacgccgtt gaacaccgag cattcaaact gctggatatc
    21661 cgggttttca gcgcgaacgg cttcgatcag atcgttcaga tcctggaaaa tcaacccgtc
    21721 agcaccgatg atctggcgaa tttcatctac ttcgcgaccg tgagcgatca gttccgtggc
    21781 gctcggcata tcaataccat aaacgttcgg gaagcgaatt tccggtgccg cagaagcgag
    21841 atacactttc ttcgctccgg cttcgcgtgc catctcgata atctgctcag aagtggtgcc
    21901 acggacgatg gagtcgtcga ccagcaggac gtttttatcg cggaactcgg cgcggttggc
    21961 gttcagttta cggcgcacgg acttacgacg cagctgctga cccggcatga tgaaggtacg
    22021 gcctacatag cggtttttaa cgaagccctg acggtacggt ttgcccagaa tacgcgcgat
    22081 ttccagcgcg atatcacaag aggtttccgg gatcgggatc accacgtcga tatccagatc
    22141 ttcccattcg cgggcaattt tctcgcccag tttcgtgccc atattcacgc gggcgctgta
    22201 aacggaaatt ttgtcgatga acgagtccgg gcgagcaaag tatacatact caaacaggca
    22261 cggattgctg accggattgt cagcacattg acgggtaaac aactgccctt cttcagtgat
    22321 gtaaatcgct tcgccaggcg cgacgtcacg caggaaatca aagcctagtg tatcgagcgc
    22381 aacgctttcg gaagcgacca tatattctgt gcggttctcg tcaatatcac gttttcccag
    22441 taccagcgga cgaatcccgt ttggatcgcg gaaagcaacc ataccatggc cgataatcat
    22501 cgccacgcag gcatacgcgc cgcggattaa gcggtttgtg gcagcaatgg cagcgaaaat
    22561 attgtcggct tccagcgggt agtggcggaa gttgtccagc tcgctggcga agatattaag
    22621 cagaatttcc gagtcggaag tggtgttgat gtggcggcgt ttttcttcaa acagtttttt
    22681 acgtaactcg tgagcattgg tcagattgcc gttgtgggca agcgtaatgc catacggaga
    22741 gttaacgtaa aacggctgcg cttcagaggc gctggagctg ccagccgtag ggtaacgcac
    22801 atgaccaatg cccatattgc cctgcaaacg ctgcatatgg cgagcttcaa atacatcgct
    22861 caccagcccg ttcgctttac gcaaacggaa gcagttattg gcatctatgg tgatgatgcc
    22921 ggcggcatcc tgaccgcgat gctgaagcac cgttaaggca tcataaatcg actggttaac
    22981 cggcataaca ccggcgatac cgacaatacc gcacatacgt ctttttcctc gttaagccac
    23041 atctcagagc acttacgctc tgggcaagaa acttgacgag ctttgcagat aatcaaaaaa
    23101 ccatctgatg ataaaactga actgcgggat cagctgtgat ttgctccagt cttcgctttt
    23161 cgacaccccg gtaaaggagt cgagaaagaa gagaatggcg gcaacaatca acacaccgcg
    23221 caacgcaccg aaacagacgc ccagcacccg atcggtgcct gacaaccccg ttttctccac
    23281 caactggcct atcacgaagt tcacgatagc accaacaatc agggtagcga taaacagtac
    23341 cgcgatggca atcccatttc gaaccagttc gtcttcaaag cccgtaaacc agactgacag
    23401 gtaagtgtag taatgactgg caacaaagaa agcacaaccc catgtcacca gcgataacgc
    23461 ttcacgaaca aagccgcgga tcaggctaac cagagaggaa aaagcaatca ccgcgattat
    23521 ggcgtaatca atccagacca tatgtgtccc acgattttac gccctgtcat cctgttcggg
    23581 gcgcattcta acagaaaaag aaaacgtttg cgtagggatt tccttcccac gcatcaataa
    23641 aaatggcgct gaaaaaatat tcaacgccat cgacttttta tgcctttgcg gcatcgggca
    23701 agaccgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagctgcttg
    23761 agctcaccca gcgatccttt cagcttctct ttggaagcat caggcccaac cagaatacgg
    23821 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    23881 agcttaccga caatctcatt cactttatcg gcatttttca gcgcgcccag ttgcacaaca
    23941 taagctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    24001 gcaggtggtg cttcaacctt cggtttaggc gactccaccg gtttcggctt cggtggggcg
    24061 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    24121 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    24181 ggcagcgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttggc
    24241 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    24301 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    24361 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    24421 cgaaccacac accagcacgg tatcttccgc tttagcgtcc gccattgcgg catcccatgc
    24481 ctgcgccacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    24541 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    24601 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    24661 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    24721 gacatcaaaa ataacacgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    24781 gctggcaatc ccgtcgcgaa tggcattttc actgacctcc agcccgctgg cacgcagtgc
    24841 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    24901 gccgtgagca tcgctaaacg tccagtcatg atcggtgacg gaatagttcc actcaacgcc
    24961 ccgacgttgt aacagtgcac ttttttcctg cgccacatca gcaatggtag aaggcatttc
    25021 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat
    25081 gctttcgcga tctggcccca gccagtcggt atgatccagc gcaatgctgg ttacaaccgc
    25141 gacatcggca tcgacaatat tggttgcgtc cagacgacca cccagcccta cttccagaat
    25201 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    25261 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    25321 cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacgca ccagatgcgg
    25381 tgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    25441 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac
    25501 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    25561 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    25621 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    25681 ccctgccctt atcagttatc aggcctcagg ttcctgatct ggtaccggtg gcaccactac
    25741 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatact
    25801 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ccttctcgat
    25861 caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct gttcgataac
    25921 acgcggaccg gcaaagccga ttaacgcttt cggttcagcg atgttgagat cgcccagcat
    25981 ggcgaaactt gcagaaacac cacccatggt cgggtcggtc agcacggaga tgtacggcaa
    26041 gccgcgctcc tgcatttttg ccagcgccgc agaggttttc gccatctgca tcagcgacat
    26101 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagtg ggcagttatc
    26161 ttccagcgcc tgctcaacgg cacgcacgaa acgtgcacct accacagacc ccatggaacc
    26221 gcccataaag gcgaactcga atgccgcagc gacaactggc attccataca gggtgccttt
    26281 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    26341 atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccagctc
    26401 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    26461 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    26521 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    26581 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    26641 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    26701 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    26761 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt ggcgaataaa ttcgaaaaca
    26821 cctggcaata ttgaaacaaa aataatgcca acaatcatca gtttaaggtt gctctgaatg
    26881 aaggggagcg ttccgaagaa ataacccgcg taggtaaaaa tcagtaccca tagcaacgcg
    26941 ccgatgacgt taaaagcggc gaaatgacga tacgacatgt ggcccattcc cgcaacgaac
    27001 ggcgcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    27061 ttctcgtaaa actgatgagt cttatcaaga taactgcgac gaaagatttt cgaatccgga
    27121 ttactgaaca gtttgtcacc aaataaccgt ccaatggtgt agttcaccgc gtcacccaca
    27181 atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt
    27241 gatgccaacg caccggcaac aaacagcaat gaatcacccg gtaaaaacgg cgttaccacc
    27301 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccatactcc
    27361 gcgaccagtt ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    27421 aggtccatat tagcctatgc cttgtactcg tcatcttaca ggctgtaact acgttggcta
    27481 cagcctgaaa tgttccgagc attattcttg ttagtccgcc agaaatagcg ggcccattgg
    27541 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    27601 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    27661 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    27721 atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac gcgtgacatt
    27781 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    27841 gaaatcattc tcgcccagca agcattgcgc ggcccgatgc atccgttcag cgtccagcgg
    27901 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    27961 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    28021 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca acgtccacgc
    28081 agcgtctttg cgctgcgcgg tcgtttcgaa atgcacaacc tgcccggtgc cgtgtacacc
    28141 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    28201 cttttccagc ttctcctgca cactgcggac ttcgttctgc cgttgccagc cgtaatactt
    28261 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt gctggtcgga
    28321 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    28381 ccaaagcgaa cgttatcggc caccgaccag aactgaactt gctccggcat accgtagtca
    28441 ttacgtacgc agccaacgga aagatgcggc gtaccagaag catcccccac ctgagtcggg
    28501 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    28561 tctgctgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    28621 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat ttttcgtact
    28681 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcag taacggcagc
    28741 atgttgaacg ccagctgacg cccgaagaaa tcttcttcat caatcggaat gccgttgagc
    28801 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    28861 atcaggctgg taacgctgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    28921 gccagcagtt ggctggtcag actgtccggt acggcgatga cattccggtt gcggtaatcg
    28981 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcaggttc gagagcaaac
    29041 aatccgctgc tatcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    29101 gtagcttctt tgcctgcgac aaaaaacgcc agctgcgcct gcgtccagtc gaattcagcg
    29161 gcatcctgca cggtgattgt cttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    29221 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    29281 gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    29341 gtttactcca gaaatagcaa agctccctgc caatattaca gcagagagca tgaagaagag
    29401 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcgc
    29461 tggcgtcatc acaaatcaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    29521 gcagcttatc gaactcaccc ggtattccgg cgactttacg cagcggtgcg tcatcgcggc
    29581 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    29641 gcgtaatgcg gccaaactct ggcgcgggca gcaacgtatc cagcgcaaca tgctgttcat
    29701 gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca
    29761 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tttcagcaat tccacgttga
    29821 gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct tcgttcagac
    29881 aggtcaatag ggcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    29941 ctggtttcag gctacggatc agtttttcat ccgccagatg cagcgttttg tacggaccat
    30001 ctttaaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat tcatccagcg
    30061 agcggaaatc cccctcatcc ccacggtcgg cgcgaggcgg atcgcaaagc aaggttgtaa
    30121 tccctaacgc ttccagtcgc gcctgtaaac ggcgaccaac gttaccaacg cccacgatcc
    30181 ccactgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agtgaggaga
    30241 aaacatattc caccaccgca atcgcattac agcctggtgc cgcggaaaaa ccaattccca
    30301 cctgctttaa ccacgcttca tcgacatggt cggtccccgc ggtggcagtg ccaacaaatt
    30361 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    30421 catccgcgtc tgccagttga gccacgggga ttggacgccc gggaatcgcg gtcacctcgc
    30481 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    30541 ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag
    30601 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    30661 cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    30721 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    30781 caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    30841 tcaccgctgc tttcgcgtca ctttcctgcc gctgagcata atctggcgca acgtctgctg
    30901 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatctacgt
    30961 ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    31021 ctgaatcagc tctcaccgga gcaattaaaa accattctaa ccctgttgca ggaagggaag
    31081 atggttattc cgcaaccgca gcagcgcgag gcgacagatc gtccgttatt accggcggag
    31141 cacaatgcgc tcaaacagct ggtgaccaaa cttgcggcgg caacgggaga acccagcaag
    31201 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    31261 aaactgttta accatctggt gacctggctg caggcgcgtc agacgctaag ccagcaaaat
    31321 acgccgacgc ttgaatcact ccagatggcg ctaaaacaac ctttagatgc cagtgaactg
    31381 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    31441 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    31501 ccgcgagata tacaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    31561 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    31621 ctggtttgtt aaccccgacg aaacgacagt atcgtgacaa taattcccag caccgcagag
    31681 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgcaagc
    31741 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    31801 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    31861 catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcacctgg ggcttgccag
    31921 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    31981 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcacc gccaaacgcg
    32041 gtaagagtaa agcccgccat tgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    32101 ccgataaccg caaaaccaac gccttgtagt gccagaccta accctggttt ccagataagc
    32161 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    32221 ccgttacagg cccacgccag taagggtaat gccattgtgg tgagcgccag tgcggcaaaa
    32281 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    32341 gccattccgt tccatgacat cactttgccg gaatgttttg gcccaacgat gcctaatccc
    32401 caggtcagag cgcccgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg
    32461 accaacagga caaatttgaa cggtgcggag acgggcaaaa tcgccgccaa cagcagcgcg
    32521 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    32581 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatgccgacg
    32641 gcaataccga ccatggtgtt gccatagccc agttcatgat gaacaaacag cgggataacc
    32701 ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc
    32761 gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    32821 atgcagcgca aggcgaggaa tttccccgtc tcatctctct ggtttcaggg ttacggtgcg
    32881 ttggcaggat ttaacgcgca cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    32941 tggaacaggc ggcgattgtc aatgatgtga aaaagggaac catcgggttc cctttggcgt
    33001 tagtgccgga ggccgcattg gcgcgtaatg tcggatgcga cgctgacgcg tcttatccga
    33061 cctactccaa attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    33121 ccgaagctgt tagacataac ggtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    33181 agacccgcag cctgttcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    33241 tccagcatca gcagagagta gatagcttcc tgtacgccag cagcgcccag agagtgaccg
    33301 gtcattgctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagcc
    33361 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gggagttcag gtagtcgatt
    33421 ggggtatcaa caccgtgcat cgccatcttc atgcaacgca ctgcgccttc gccagacgga
    33481 gcaaccatgt ctgcgccatc agacgttgcg ccgtagccaa cgatttcagc atagatgtga
    33541 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgcctgcg
    33601 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    33661 tatttggtag acagcgcacc catcgcgtcg aattcgcaag ccatttccca gcacagctct
    33721 tcgccgccac cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    33781 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    33841 ttaaacggcg tggcgagaca ggcagaaacg ccagatgcca tcgctttagt gaccacatac
    33901 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    33961 cgcggggaac caccaccgga acctgcaatc aggcccacac gtgggttgtt ctgataagct
    34021 tccggggaga ggccagcatc tgcgattgcc tgctccatgg aaaggaatgc atagatggat
    34081 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    34141 ttgccccata cgtggctacg catgccggag tccttcagct cttgagagaa agtgatccct
    34201 gagcgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaacg
    34261 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcactatagt
    34321 aagtttcgaa tggcacaata gcgtacactt gtacgccgaa caagtccgat cagacgatta
    34381 atagagaaat ttgcgcagcc tgacacacat cgttaagatc gtcagactgc ccgtcagacg
    34441 agtaacttac gtgaaacact acgccataca acctgccaac ctcgaattta atgctgaggg
    34501 tacacctgtt tcccgagatt ttgacgacgt ttacttttcc aatgacaacg gactggaaga
    34561 gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg aacatccaca
    34621 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    34681 gcaggcattt gatcagtttc gcgaagcaca tccgcaagcg caattacaac gtttacattt
    34741 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagccc atcaacactg
    34801 gccggaactg gctcagtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    34861 ttgccatcgt ttattactcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    34921 taatgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    34981 ggacggcttt gcgccagcga aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    35041 ggcaagactg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    35101 ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aaaggctttg ggcgcaaacg
    35161 agaaatgctt tgtggagtga tggagcaagc attacccctc ccctgcgcca cgccgtggtt
    35221 taaccgtacg ggcagtagca aacgggaagc ggcgattatc ggcggcggta ttgccagcgc
    35281 gttattgtcg ctggcgctat tacggcgcgg ctggcaggta gcactttatt gcgcggatga
    35341 agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ttgtatccgt tattaagcaa
    35401 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    35461 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    35521 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg aagaactggc
    35581 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttacgaaaa attgcagcgg
    35641 tattacttat ccgcaaggtg gctggctgtg tccggcagaa ctgacccgta atgtgctgga
    35701 actggcgcaa cagcagggtt tgcagattca ttatcaatat cagttacagg atttatcccg
    35761 taaggatgat tgttggttgt tgaattttgc aggtgatcag caagcgacgc atagtgtggt
    35821 ggtactggcg aatgggcatc aaatcagccg tttcagccaa acgtcgtctc tgccagtgta
    35881 ttcagttgct ggtcaggtca gccatattcc gacaacgccg gaactggcaa agctgaagca
    35941 ggtgctgtgc tatgacggtt acctcacgcc gcaaaatccg gcgaatcaac accattgtat
    36001 tggtgccagt tatcatcgcg gcagcgaaga tacagcgtac agtgaggacg atcagcagca
    36061 gaatcgccag cggttgattg attgtttccc gcaggcgcag tgggcaaaag aggttgatgt
    36121 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccact cgcgatcatt tgccaatggt
    36181 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgctgg cggagcagaa
    36241 agataaggcg gtaagcgcgc cggtttatga cgatctcttt atgcttgctg ctttgggttc
    36301 gcgcggtttg tgttctgccc cgctgtgtgc tgagattctg gcggcgcaga tgagcgacga
    36361 accgattccg atggatgcca gtacgctggc ggcgttaaat ccgaatcggt tatgggtgcg
    36421 gaaattgttg aagggtaaag cgattaaggc ggggtaatct actttggcag tgtttgccgg
    36481 atgcggcgtg aacgccttat ccggcacatt acttactgct gtgacgcctg ctgaaacagg
    36541 ttttcccaca tatcggtcac cagtgcctgg tcacgcggcg acaattcacc ggcaccaatg
    36601 gctttttcca gactctggct aacggttgta tgtaccgcct gggcggagtg gtcatcacca
    36661 ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac
    36721 tcatcatcac tggcatggtc aaccataccg tcgattaatg ccagaatgcg tgattcaaac
    36781 tccgcgatca tcttctttcc tcattgtaaa acgtggcgca ggcttcagtt gagcgcttcc
    36841 ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg taatgcttta
    36901 atgaagcgtg ccgggcgttc aggaatgccg ttttccagat aatccatcac ctgtgcatgt
    36961 acccgccgtt gaaataccac gcggtccggt tcgaaatccc cttccagatt gtcgcaactg
    37021 acattaaacg gatatcccgc cgccacacag aacaaccagt ccagcgcctg cggcttcact
    37081 tcaacatctt caaactggct ttgcgtttgg gcatcgcgtc cgtcgggaca ataccagtag
    37141 ccgaaatcaa ccagctcacg gcgcgctttc ccggcaatac accagtgcga aatctcatga
    37201 attgcgctgg cataaaagcc atgagcaaag acgattcggt tatacggtac ttccgcatca
    37261 gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa atcatcggca
    37321 aagcagctat taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca
    37381 tccccaacca gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca
    37441 ccgccgagac gataacgatc atcgggcgga tcagcttttg tcctttgctc aacaccagtc
    37501 gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat cacgaatcct gtcgcccaaa
    37561 tcactttgcc accgagaata aacagcagta aaccgccgat atttgacgtt gcgttgagta
    37621 atttggcgtg agccgtggct ttggcgaggt tgaatccgca cagcgtaaca aaggccagcg
    37681 cgtaaaacga tcccgctgcc ggaccaaaga agccgtcata aaaaccgacg cagccaccgg
    37741 caattaacgc gaagggtaaa ccgtacatcc gacgctggcg atcttcttca cccagctttg
    37801 gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga
    37861 catcagcctg aacatactgc accagcaatg cgccgctcat tgagccgaca aaggtcatgg
    37921 cgatattgag tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg
    37981 cggaaataga gccaccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca
    38041 tccccgccgc catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga
    38101 taaatcccgc cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa
    38161 acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg
    38221 cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cccggttttg gaggttcaaa
    38281 ccagctttgc agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca
    38341 ctccagacta tcggcaggac aacgtaatcg tacatgcata tgcgcgcgat gctggaacca
    38401 gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt
    38461 aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa
    38521 gctgaaaatt tctggcttcc acagcgtcgg aacaacgtgt ttaccgtcgc gggaaactaa
    38581 atctaacgct tgcgggcgca agagctgcgc ggaggtccag cgtgtttttg gcagttgcag
    38641 gaagatgtcc acatccagtc cggtctggtg gctggcgtga ccgccgttga aacgaccacc
    38701 agcaggcatc cccatatcgc caatcagcac cgtgcccaag cccagattgc tcacctggct
    38761 actcagacgc tggataaaca tcaccagatc cggatgtccg aaatagcgac gctgatcggt
    38821 acgcatgacc tgataatgtt cggactgtat cggcagcgtg tcagcgccga cgatacaacc
    38881 attagaaaaa ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca
    38941 cggcgtcgct gccaggctgg cgctactggc aagcagggcc agcagcgcaa tcgcggtttt
    39001 gttcattttt taccagcgtg gaatatcagt cttcacatcg acattttgcg cccgttgccg
    39061 taacaggtga tccatcaaaa cgatcgccag catcgcttct gcgatcggca ccgcacggat
    39121 cccgacacaa ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt
    39181 aatggtgcgc cctggcacgg taatgctgga ggtcggcttc agcgccatat gggcaatgat
    39241 ttgctgcccg ctgctgatac cgccaagaat gccgcccgca tggttgctct ggaaaccgtc
    39301 tttggtgatt tcgtcgcggt tctggctgcc acgcagcgcc accacgtcaa aaccgtcgcc
    39361 aatttccacg cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag
    39421 gcggtcaaag accggctcgc caagtccggc gggtacgcca ctggcaacga cggtgacttt
    39481 cgcgccaata gagtcaccct cttttttcag cgcgcgcatc agttcatcta acgcgtcaat
    39541 tttgtccgga tccgggcaga agaatggatt ttgctcgacc tgcgaccagt ctttgatttc
    39601 cagcggaatg tcgcccatct gggtcaggca gccgcgaatc tcaatgccaa atttctcggc
    39661 gagatatttt ttggcaatcg cccccgccgc cacgcgcatg gcggtttcgc gggcggaaga
    39721 acgtccaccg ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcggcatg
    39781 gcctggacgg aaaacgtctt taatggcgct gtaatcctga gaacgctggt cggtattttc
    39841 gatcaacaag ccaatgctgg tgccggtagt aacgccttca aaaacaccgg agagaatttt
    39901 gacctgatcc ggctcacgac gctgggtggt gtagcgcgat gtcccagggc gacgacgatc
    39961 aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat
    40021 gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat
    40081 tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat
    40141 aaatcgcgaa atgttcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat
    40201 cgccgccgtt atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat
    40261 gtaccatgct gttgccgact tcacaaatca gtacgccatc atcagcaaga taatctgccg
    40321 cgttaccgag aatgcgacgc gtcagtttca ggccgtcagt gccagatgcc aggcccagtt
    40381 ccggctcgtg acggtactca tttggcaggt cggacatatc ttccgcatcg acatacggcg
    40441 ggttagtgac aatcaggtcg tactgcactt tcggcaagtc gcggaacaga tcggagcgaa
    40501 tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcagca accgccagcg
    40561 cgtcgggaga gatatccacc gcgtcaactt ccgcatccgg gaaggcataa gcacaggcaa
    40621 tggcgatgca gccgctaccg gtacacatat ctaaaatatg ctgcggttgc ttgctgataa
    40681 gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt
    40741 catcgacgta aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa
    40801 tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg gtgtttttcg ctggaggtca
    40861 gacgcgcggt gcgcatatct tccgggatat ccagcggcag gtagagcgaa ggcaacacca
    40921 gctgtacggc ttcatcccac gggttgtcag tgccgtgacc gtaccagata tttgccgcgc
    40981 tgaagcggct caccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt
    41041 catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc
    41101 tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac
    41161 gtttaaaacg atcgattgcg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga
    41221 cagataaatg aaaaagaaaa caacacttag cgaggaggac caggcgctgt ttcgccagtt
    41281 gatggcgggg actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaagaa
    41341 aatcagcgaa gtgccggtga aacggttgat tcaggagcag gccgatgcca gccattattt
    41401 ttccgatgaa tttcagccgt tattgaatac cgaaggtccg gtgaaatatg ttcgcccgga
    41461 tgtaagccat tttgaggcga agaagttacg ccgtggcgat tattcaccgg agctgttttt
    41521 ggatttgcac ggtttgacgc agctgcaagc caagcaggag ttaggcgcat tgattgccgc
    41581 ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat ggtcatggga agcatatttt
    41641 gaagcagcaa acgccgctgt ggctggcgca acatccgcat gtgatggcat ttcaccaggc
    41701 accaaaagag tacggtggtg atgcggcatt gctggtgttg attgaagtgg atgagtggct
    41761 gccaccggaa ttaccgtgaa tgctggcatc gaatgcctga taagacgctt cagcgtcgca
    41821 tcaggcatca gtgcacaacg acgagctact acagcatatt cagtacgtac tggaatcgac
    41881 aggtagaatc aataggttgc cgcgaggtgg tatccacgcg aagcaccata tcatgaagct
    41941 ctttgtcggt aatggggtga cgatttggtg ttaccgtcac tttggcattg tcacagcccg
    42001 atgaggtgaa ggtgagcatc tgcgacaccc aacgacaata agcaccacga tcgtcagtat
    42061 caccaaattt gctgccgtca cacagtttgc cgtctttcat caggtaggtg gagagcgttt
    42121 tttcaattat cccgccggta gactggagcg ctaacgtctt atcacgcaca acccgacttt
    42181 cattccagga gacatcataa ggccctaacg gtataggtgg acaagaagag ttactgactg
    42241 tgcgtccgga ataggttccc ggagcaggat caataagatc aatgctatct gcatgtgtcg
    42301 ataccagcca cgggcagcca tgaacgctgg agtcttcctt ccagacttcc attcgtattt
    42361 cataacaagt ccacatcgtc ctttgcgcac ggctgtcata ctctgcaagc cccggacaag
    42421 atgattgtga tatcgctgtc atcgatatga cttttaatga tggtgcaggg ccaggtcccg
    42481 tccagctttt caaaggtatc catccagtga ttaaaccggt gttatttggt gtctcataaa
    42541 caccgtgatt gacgctatcg ggggtaagaa catcaagata ataagttatc ttcgttgtgt
    42601 agcttgcata ccctatcggt gtatacattg ttgcaacaat gacattcgcc tgaactgctg
    42661 tactgaagaa aagtgatatc agcaggccca aaatccagcg cattattttc atcttaaata
    42721 tactcaaacg ttgcggttaa ctgggcggta aaatcaccag gtgttacatc acgcccctct
    42781 tttgcctgta accatgctct gaaattaaga ctgttattgc catcattcag cacaaaggtc
    42841 atccccgtcg tgttattaat agcaaccgca gtaccgtcag tagtttccat accaatgcct
    42901 acgccctgtg caccagaggt atcatccaga gccagaaatc ccggttgccc actgtcttct
    42961 gttccggtca gggtaacttt tacgctgtac tgcgctggcc cacggcaatc tttcaggcga
    43021 aaaaccaccg gtacacgatc ggactgaccg cgcaccatca aatctttgta gctgatatca
    43081 gcaaactgta cctctgccaa cgttccagcc tgttccaccg gagtacaggt tttgctcatc
    43141 aggttgccgt aaaaatgcaa attattagct gccagtgctg aatgactcgc cagcaccagc
    43201 acaccgccca acagaagggt ttgcaccctg atacattgat atttcttcag tttgttcatt
    43261 gataatccac ctgcaatgtg gcgtaagcgg aaaagttacc gtcagtcagc gtggcatcgg
    43321 aagcttttac aggtgcggcc ttcaacttcg gcaggttggc gggtgtcacg ttaagcgggg
    43381 tattcagctt aaagggctga ccgttttgct ggagttcaat ccctaatccc gtgacatccg
    43441 tctgaatcgc cgcatcgtta tagttggtct gtgtaccggt ccaggttagc gtcattaccc
    43501 aggcgtcatc ggtaatatca gggtcacacg ttatctggta cggaacatct ttgcggtaat
    43561 taaccccgtc aatattgtcg ataatgacgt cgataaactg gatctcaata tcatcaccat
    43621 tgctgacggt gcagaccgga ggttcgatca gcgtacccgt gaaggtgata tcttccggtg
    43681 cggcatgcgt tatggtgcaa aaagcggata ataaaattaa cgagagtcgt ttcattggta
    43741 atccaccacc attgtcatgc tggcgttaaa ctgcgcgcct gtcagcgtca cgccattctg
    43801 tttgactggc actgctttta gttgtggctg gctactgacg ctaaatggag tccaggtatt
    43861 gtcgccaatc gcaaacagag agttatcggc tgcattttca atacgaatgc caaacccggt
    43921 aattcccgtg cttaacaccg tttcgccatt gattgtggtg gttgtgccct taaactgcat
    43981 ctgaaggtca tcgctcaggc tattggcaca gttcagggta taacctgcat ctttgcgata
    44041 ttcagtacca ttgatttttt tgatgatcac atcaccaaaa tcgacgttac tgccttttac
    44101 ggtacaagga ggtggtgggt ctaccagtac gcgcaatgtc aatggcacac ttttcgtttc
    44161 accggcaaga gcactgcccg taagcagcac tcctcccagc ccggccaaaa gtagtcgttt
    44221 catctcacac tcccggcctc agcctttgcg gctctgctct tcatcaacct ggcaactgtt
    44281 accagtgcat ttgaagaaca gcggcatccg tgcgccatag tcgttgacgt atgtcagcac
    44341 cggcacactc tccatcttca cgttcagcgg ggccgaggtt tttggcgaca tcactattgg
    44401 cgagaaaccg gctgccggat tgccattttt ctgtgcgctg gcattactga aaatgacgta
    44461 atacggcgtc gggttattga cagtaaattc ctgaccgttg tgggttaagg tcactttatg
    44521 ctgccacggg ttttgcgtgg tgtttttttc cagagctttc ggtcgccaga aaagtttgat
    44581 acgcgtctga agagcgattt gtagcgtgtt ggatttgttc gatttcggcg gaatttcgcg
    44641 cacgttgaaa tagaacatcg attcccgatc ggcaggtaat ttatgaatat ccggcaggcc
    44701 ctgaatttta acctggccat tcatcattgc ttcaatgcgc tgtacaggcg gcaataccgc
    44761 cagcggcgag ctgatcttga cgcccttttc atcttcaatc cagctttgcg ccagataagg
    44821 cagtttgggg tcgttattac gcagcgtcac gctgatagat ttatcgcttt cattgaaaat
    44881 cagccgggta cgatcgggcg ttactgacgc ctgagcagaa aaacttaccg ttgctgccag
    44941 taacgacagt accgttgtca atgttttggc agagagatat ttagtcatgg atcattcctt
    45001 aatcacgtta ttggttcgat gaaacagaca tcggcggttc cattggtgtc acctgttgtg
    45061 tctgctcctg aatgacgggt tgaatatcat gcggtatgac gggagaactt cccccctttt
    45121 gctggcatgg caacaacaag ccattgaaca ggtcattcgg taacgggtct ggcaggctga
    45181 tatcgcagtg agattcaccc tcccagaaaa cggtcatatg ttcacccggt ttaacgcctg
    45241 ccagataaac attaccttcg tcatcaacca accctacttg ttgttgatta tcgtttttca
    45301 cttccgcccc aaatggcgga tggctgccat cctgcaagcg cagcactgcc atcgccttct
    45361 caccgctgat taccgcaaat ttacggtagc caatagcgcc ttccgtcagc gttgcctgaa
    45421 ccactgattt tgtagcttcg gcgttttccg gcagtttgtt caaatcgata taagcctggt
    45481 tgcggtagta atcattaaca tctgccacca ccgctttgcc gaacatgttg gtataaacgg
    45541 cggaaccatt accttcaacc ggcacattag caacgccatc agcgtcgatt aacagacgtg
    45601 taccgcccat gttttgtgta cgatgcagtg ccccgccatg agcggtcagc gttgccccgc
    45661 cctgtaaaga gatccctgct gaggtgtatt gcccttcatg gtagttagca ctgagatcaa
    45721 cttttgccat agtgccatcg tggctgtagt agccatccac gctgctgtgg ttttcgctgg
    45781 ttccggcgct taacgtgtag tggctggcgt catcaatgcg gtgatacaga ctcacctgat
    45841 tgctgtcggc accgctgcca tagttgccgt tgtaactgat ggtgctgttg tcgccccacg
    45901 gcaaacttaa ggacagataa acacccttgt cggcactttt atcgtattcg tagcgataac
    45961 cggtcagaga aacgctcagg ttgcgaaggc ttcctaaatt gaaataatga gaaagcatca
    46021 tgttgtagtt ggtctgatcc tggcgatccc agtaggtatg gtgggaatag ttgagataga
    46081 ccgaaacgcg cgcatccgtg aagttctggt tataggtgac cgtatacatc tctttgtcgt
    46141 taccggtgcg cgcccggtcg tcgtcgttcg catccagata ctcgctcatg gtcatgtagt
    46201 ttttttcaga gaagcggtat ccagcaaacg tcacccgact gttgagttca tcaaaatctt
    46261 tggcatagct gacgcgataa gagttgccgt ccagcgtttt gttgccatac acgctgttat
    46321 cgtcgagttg aacacgagag tgagtgacat caaacgctaa cgcgcctaac agcgccaggt
    46381 cacgaccgag tcccagcgcc gccgactggt agttttcatc ggctaacgca ccgccgtaga
    46441 gcgaccagcc attggcaatc ccccaggatg cctcgccgcc cgagaagaag ctacctgcaa
    46501 cctgatggtc ccagttttgt ggacgcccca ttgtcacttt ataacgcacc tggccggggc
    46561 gagtcaggaa cggcatggac gctgtgctga cgtcgtattc ttgtacctgg ccgttttgtt
    46621 cttcaatgcg gacatgcagc gtgccagaga cggagtcgcc aatatcctga atgcggaatg
    46681 gccctgcggg cacctgggtt tcgtaaatga cacggcccat ttgggttaca gtcactttag
    46741 cggtagtgtg tgcaacacct gaaatatcgg gggcgtagcc gcgcaggttt ggcggtaaca
    46801 tttgatcatc ggtactgatg ctgccgccaa tgtaactgaa gccatcaaaa atatcggagt
    46861 tgagataatc ttcgcccagc gaaagtttgg cttttaatga cggcaaggcg cgccaggcgt
    46921 agtaacgact ccactcccag tttttttgcg taccgctatc gtcagtgtcg ccgtcatcgt
    46981 tactgcgggt atgctgataa tcgctctgcc agtcagctcg taagcgccac ggcccaacgt
    47041 taacgccgac cgtaccatta ccgctgatgt cgttagtgtc atcaccccca ttttcatgtc
    47101 gggtttgcgc gttgatactg taatcggcaa taagcccagg gataccgtca tcccagcgcg
    47161 aaggcgggtc ccactcgctg tcggtatatt ccagataagc ctgcggtaca gaaacaagga
    47221 gtgcagactg gcctaaatcg ccgctaattt ctgtacctgc taaaagtgcc gtattcagac
    47281 actgaccatc gcggatccat tgcaaattct tcgcaatatc ttccttcaga ccaaactggg
    47341 caaccagctc tggcgataag caggcatatg atttattcgg atcgttttca gttgcgtacc
    47401 agtagatatc gtagtcgtca ggtaacggct gattatttac atgaacgcgt aagttatatt
    47461 ttcccggttc tacatagcct ttttgcgaaa aacggcccaa atcaattttg gtattgcctt
    47521 ttaaatcaag aaatcggcca tcaaactgaa tggtatcgtc tgccagggca ttaaatggac
    47581 ttccagaaat tgccagagca atccaccacg aaagcccccg taaacggaga aaagaatgtt
    47641 ctggcatcgg cgtatccatc catgattttt tataatttag ttatttggta gttataaacc
    47701 ccataacttt gcagctatgg gggaaataca ttagagatac gtgatctgga acgtcgtcag
    47761 cgtttcaaac ggtgccggtg ttggtgttac agcatccgtt tcacccacta accatgcttt
    47821 aaagttcaga gtttgtttag gcttgccttt tcccgttgaa ctgtccttca cgataggttg
    47881 cttaatctct gcccccagtg cataacctgt accgtgctgg tcaccgataa ccaggctaac
    47941 gtttggaatt tcagttccat tatccatgtt agacagagaa tagttatctg cgttagtgga
    48001 gtacggatta ccggtgaatg tggtagccat tgtcgtttct gtattgaaat tacagtcctg
    48061 taaacgaatc tggaaatctt ttggtgtggt tgttttgccg ccggttaaag aacccgtacc
    48121 gatgctgccc atatccactt ccagtttatg atcgtcagga acaattgagc acggggagtc
    48181 ttcaattgtg ccgtaaaatc tcactgtacc gttgttccct gcggcaacga ctgatgctga
    48241 agtcagcgcc cccatgagca ttgccgtggc aactgctgct tttacaaact tactcattat
    48301 ttgtccttaa atcataaata attagaatat aagccaactg aaatattgac acagag
//
LOCUS       DARNVM010000014        46284 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.014, whole genome shotgun sequence.
ACCESSION   DARNVM010000014 DARNVM010000000
VERSION     DARNVM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.014"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            310..4053
                     /locus_tag="SI621_000802"
     CDS             310..4053
                     /locus_tag="SI621_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEI4063555.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPIEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     ILLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAKKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATFERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            4050..5588
                     /gene="narH"
                     /locus_tag="SI621_000803"
     CDS             4050..5588
                     /gene="narH"
                     /locus_tag="SI621_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEI4063556.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5585..6295
                     /gene="narJ"
                     /locus_tag="SI621_000804"
     CDS             5585..6295
                     /gene="narJ"
                     /locus_tag="SI621_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEI4063557.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLCARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6295..6972
                     /gene="narI"
                     /locus_tag="SI621_000805"
     CDS             6295..6972
                     /gene="narI"
                     /locus_tag="SI621_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEI4063558.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7157..7288)
                     /locus_tag="SI621_000806"
     ncRNA           complement(7157..7288)
                     /ncRNA_class="other"
                     /locus_tag="SI621_000806"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7336..7467)
                     /locus_tag="SI621_000807"
     ncRNA           complement(7336..7467)
                     /ncRNA_class="other"
                     /locus_tag="SI621_000807"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7514..7645)
                     /locus_tag="SI621_000808"
     ncRNA           complement(7514..7645)
                     /ncRNA_class="other"
                     /locus_tag="SI621_000808"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7692..7776)
                     /locus_tag="SI621_000809"
     tRNA            complement(7692..7776)
                     /locus_tag="SI621_000809"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7740..7742),aa:Tyr,seq:gta)
     gene            complement(7811..7895)
                     /locus_tag="SI621_000810"
     tRNA            complement(7811..7895)
                     /locus_tag="SI621_000810"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7859..7861),aa:Tyr,seq:gta)
     gene            complement(8054..8896)
                     /gene="purU"
                     /locus_tag="SI621_000811"
     CDS             complement(8054..8896)
                     /gene="purU"
                     /locus_tag="SI621_000811"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEI4063559.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(8946..9404)
                     /locus_tag="SI621_000812"
     CDS             complement(8946..9404)
                     /locus_tag="SI621_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEI4063560.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARYTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9517..10422
                     /gene="rssA"
                     /locus_tag="SI621_000813"
     CDS             9517..10422
                     /gene="rssA"
                     /locus_tag="SI621_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEI4063561.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10514..11527
                     /gene="rssB"
                     /locus_tag="SI621_000814"
     CDS             10514..11527
                     /gene="rssB"
                     /locus_tag="SI621_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HEI4063562.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            11729..12637
                     /gene="galU"
                     /locus_tag="SI621_000815"
     CDS             11729..12637
                     /gene="galU"
                     /locus_tag="SI621_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEI4063563.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(12782..13195)
                     /gene="hns"
                     /locus_tag="SI621_000816"
     CDS             complement(12782..13195)
                     /gene="hns"
                     /locus_tag="SI621_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HEI4063564.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            13799..14416
                     /gene="tdk"
                     /locus_tag="SI621_000817"
     CDS             13799..14416
                     /gene="tdk"
                     /locus_tag="SI621_000817"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEI4063565.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(14719..17394)
                     /gene="adhE"
                     /locus_tag="SI621_000818"
                     /gene_synonym="adhC"
     CDS             complement(14719..17394)
                     /gene="adhE"
                     /locus_tag="SI621_000818"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEI4063566.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            17871..18518
                     /gene="ychE"
                     /locus_tag="SI621_000819"
     CDS             17871..18518
                     /gene="ychE"
                     /locus_tag="SI621_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HEI4063567.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(18676..18972)
                     /locus_tag="SI621_000820"
     CDS             complement(18676..18972)
                     /locus_tag="SI621_000820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063568.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLLIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            19256..20887
                     /gene="oppA"
                     /locus_tag="SI621_000821"
     CDS             19256..20887
                     /gene="oppA"
                     /locus_tag="SI621_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HEI4063569.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            20973..21893
                     /gene="oppB"
                     /locus_tag="SI621_000822"
     CDS             20973..21893
                     /gene="oppB"
                     /locus_tag="SI621_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HEI4063570.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            21908..22816
                     /gene="oppC"
                     /locus_tag="SI621_000823"
     CDS             21908..22816
                     /gene="oppC"
                     /locus_tag="SI621_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HEI4063571.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            22828..23841
                     /gene="oppD"
                     /locus_tag="SI621_000824"
     CDS             22828..23841
                     /gene="oppD"
                     /locus_tag="SI621_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HEI4063572.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            23838..24842
                     /gene="oppF"
                     /locus_tag="SI621_000825"
     CDS             23838..24842
                     /gene="oppF"
                     /locus_tag="SI621_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HEI4063573.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGNYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(24895..25224)
                     /locus_tag="SI621_000826"
     CDS             complement(24895..25224)
                     /locus_tag="SI621_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HEI4063574.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(25259..26719)
                     /gene="cls"
                     /locus_tag="SI621_000827"
     CDS             complement(25259..26719)
                     /gene="cls"
                     /locus_tag="SI621_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEI4063575.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            26862..27035
                     /locus_tag="SI621_000828"
     CDS             26862..27035
                     /locus_tag="SI621_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HEI4063576.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(27090..28343)
                     /gene="kch"
                     /locus_tag="SI621_000829"
     CDS             complement(27090..28343)
                     /gene="kch"
                     /locus_tag="SI621_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HEI4063577.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(28644..28940)
                     /locus_tag="SI621_000830"
     CDS             complement(28644..28940)
                     /locus_tag="SI621_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEI4063578.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            29164..29883
                     /gene="tonB"
                     /locus_tag="SI621_000831"
     CDS             29164..29883
                     /gene="tonB"
                     /locus_tag="SI621_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HEI4063579.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(29923..30321)
                     /gene="yciA"
                     /locus_tag="SI621_000832"
     CDS             complement(29923..30321)
                     /gene="yciA"
                     /locus_tag="SI621_000832"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEI4063580.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(30426..30965)
                     /locus_tag="SI621_000833"
     CDS             complement(30426..30965)
                     /locus_tag="SI621_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEI4063581.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATALVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(30995..31738)
                     /locus_tag="SI621_000834"
     CDS             complement(30995..31738)
                     /locus_tag="SI621_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HEI4063582.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(31766..31855)
                     /locus_tag="SI621_000835"
                     /pseudo
     CDS             complement(31766..31855)
                     /locus_tag="SI621_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            32095..32733
                     /gene="ompW"
                     /locus_tag="SI621_000836"
     CDS             32095..32733
                     /gene="ompW"
                     /locus_tag="SI621_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HEI4063583.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(32793..33299)
                     /locus_tag="SI621_000837"
     CDS             complement(32793..33299)
                     /locus_tag="SI621_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEI4063584.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKEMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(33345..33845)
                     /locus_tag="SI621_000838"
     CDS             complement(33345..33845)
                     /locus_tag="SI621_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEI4063585.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(33931..34110)
                     /locus_tag="SI621_000839"
     CDS             complement(33931..34110)
                     /locus_tag="SI621_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEI4063586.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(34491..35297)
                     /gene="trpA"
                     /locus_tag="SI621_000840"
     CDS             complement(34491..35297)
                     /gene="trpA"
                     /locus_tag="SI621_000840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEI4063587.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(35297..36490)
                     /gene="trpB"
                     /locus_tag="SI621_000841"
     CDS             complement(35297..36490)
                     /gene="trpB"
                     /locus_tag="SI621_000841"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEI4063588.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(36502..37863)
                     /gene="trpCF"
                     /locus_tag="SI621_000842"
     CDS             complement(36502..37863)
                     /gene="trpCF"
                     /locus_tag="SI621_000842"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HEI4063589.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEIQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDARAAYDAG
                     AIYGGLIFVTTSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(37864..39459)
                     /gene="trpD"
                     /locus_tag="SI621_000843"
     CDS             complement(37864..39459)
                     /gene="trpD"
                     /locus_tag="SI621_000843"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HEI4063590.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(39459..41021)
                     /gene="trpE"
                     /locus_tag="SI621_000844"
     CDS             complement(39459..41021)
                     /gene="trpE"
                     /locus_tag="SI621_000844"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEI4063591.1"
                     /translation="MQTQKPTLELLTCEGTYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(41043..41136)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(41113..41157)
                     /gene="trpL"
                     /locus_tag="SI621_000845"
     CDS             complement(41113..41157)
                     /gene="trpL"
                     /locus_tag="SI621_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HEI4063592.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            41295..42176
                     /gene="yciV"
                     /locus_tag="SI621_000846"
     CDS             41295..42176
                     /gene="yciV"
                     /locus_tag="SI621_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HEI4063593.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            42173..42793
                     /locus_tag="SI621_000847"
     CDS             42173..42793
                     /locus_tag="SI621_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEI4063594.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            42821..44716
                     /locus_tag="SI621_000848"
     CDS             42821..44716
                     /locus_tag="SI621_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEI4063595.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QLDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNKWPF
                     EIYKTRFSLQISNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPALQTTNIFSHLLLPSTSSLLIWFPCLLMVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVVLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAERITPSLTAAVSAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            44927..45802
                     /gene="rluB"
                     /locus_tag="SI621_000849"
     CDS             44927..45802
                     /gene="rluB"
                     /locus_tag="SI621_000849"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HEI4063596.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            45972..>46284
                     /locus_tag="SI621_000850"
     CDS             45972..>46284
                     /locus_tag="SI621_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063597.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KE"
BASE COUNT        11595 a        11091 c        11865 g        11733 t
ORIGIN      
        1 tggcttattt cgccggattt cattaagagc cattaatatg ttacccatgg ggaatactcc
       61 ttaataccca tctgcataaa aatcttaata gtttaaataa ctacaggtat aaaacgtctt
      121 aatttacagt ctgttatgtt gtggctgtta attatcctaa aggggtatct taggaattta
      181 ctttattttt catccccatc actcttgatc gttatcaatt ctcacgctgt ttcagagcgt
      241 taccttgccc ttaaacatta gcaatgtcga tttatcagag ggccgacagg ctcccacagg
      301 agaaaaccga tgagtaaatt cctggaccgg tttcgctact tcaaacagaa gggtgaaacc
      361 tttgccgatg ggcatggcca gcttctcaat accaaccgtg actgggagga tggatatcgc
      421 cagcgttggc agcatgacaa aatcgtccgc tctacccacg gggtaaactg caccggctcc
      481 tgcagctgga aaatctacgt caaaaacggt ctggtgacct gggaaaccca gcagactgac
      541 tatcctcgta cccgtccgga tctgccaaac catgaacctc gcggctgccc gcgcggtgcc
      601 agctactcct ggtatcttta cagtgccaac cgcctgaaat acccgatgat gcgcaaacgc
      661 ctgatgaaaa tgtggcgtga agcgaaggcg ctgcatagcg atccgattga ggcatgggct
      721 tctatcattg aagacgccga taaagcgaaa agctttaagc aggcgcgtgg acgcggtggt
      781 tttgttcgtt cttcctggca ggaagtgaac gaactgatcg ccgcatctaa cgtttacacc
      841 atcaaaaact acggcccgga ccgtgtcgct ggcttctcgc caattccggc aatgtcgatg
      901 gtttcttacg cgtcgggtgc gcgttatctc tcgctgcttg gcggtacttg cttaagcttc
      961 tacgactggt actgcgacct gcctcctgcg tctccgcaaa cctggggcga gcagactgac
     1021 gtaccggaat ctgctgactg gtacaactcc agctacatca tcgcctgggg ttcaaacgtg
     1081 ccgcagacgc gtactccgga tgctcacttc tttactgaag tgcgttacaa aggcaccaaa
     1141 actgttgccg tcacaccgga ctacgctgaa atcgccaaac tgtgcgatct gtggctggca
     1201 ccgaaacagg gcaccgacgc agcaatggcg ctggcgatgg gccacgtaat gctgcgtgaa
     1261 ttccacctcg acaacccaag ccagtatttc accgactatg tgcgtcgcta caccgacatg
     1321 ccgatgctgg tgatgctgga agaacgcgac ggttactacg ccgcaggtcg tatgctgcgt
     1381 gctgctgatc tggtggatgc gctgggccag gaaaacaatc cggaatggaa aactgtcgcc
     1441 tttaatacca atggcgaaat ggttgcgccg aacggttcta ttggcttccg ctggggcgag
     1501 aagggcaaat ggaatcttga gcagcgcgac ggcaaaactg gcgaagaaac cgagctgcaa
     1561 ctgagtctgc tgggtagcca ggatgagatc gctgaggtgg gcttcccgta ctttggtggc
     1621 gacggcacgg aacacttcaa caaagtggag ctggaaaaca ttctgctgca caaactgccg
     1681 gtaaaacgcc tgcaactggc tgatggcagc accgcactgg tgaccaccgt ttatgatctg
     1741 acactggcaa actacggtct ggaacgtggc ctgaacgacg ttaactgcgc aaccagctat
     1801 gacgatgtga aagcttacac tccggcgtgg gccgagcaaa tcactggcgt ttctcgtagc
     1861 cagattatcc gtattgcccg tgaatttgcc gataacgctg acaagacgca cggtcgttcg
     1921 atgattatcg tcggtgcggg gctgaaccac tggtatcacc tcgatatgaa ctatcgtggt
     1981 ctgatcaaca tgctgatttt ctgcggctgt gtcggtcaga gcgggggtgg ctgggcgcac
     2041 tatgtaggtc aggaaaaact gcgtccgcaa accggctggc agccgctggc gtttgctctt
     2101 gactggcagc gtccggcgcg tcacatgaac agcacctctt atttctataa ccactccagc
     2161 cagtggcgtt atgaaaccgt gaccgcagaa gagttgttgt cgccgatggc ggataaatcc
     2221 cgttataccg gacacttgat cgactttaac gtccgtgcag aacgtatggg ctggctgccg
     2281 tctgcgccgc agttaggcac taacccgcta actatcgctg gcgaagcgaa aaaagcgggc
     2341 atgaatccgg tggattacac cgtgaaatcc ctgaaagagg gttctatccg ttttgcggca
     2401 gaacaaccag aaaacggtaa aaaccacccg cgtaacctgt tcatctggcg ttctaacctg
     2461 ctcggttctt ccggtaaagg tcatgagttt atgctcaagt acctgctggg gacggagcac
     2521 ggtatccagg gcaaagacct ggggcagcag ggcggcgtga agccggaaga agtggactgg
     2581 caggacaacg gtctggaagg caagctggat ctggtggtga cgctggactt ccgtctgtcg
     2641 agcacctgtc tctattccga catcattttg ccgacggcga cctggtacga aaaagacgac
     2701 atgaatactt cggatatgca tccgtttatt cacccgctgt ctgcggcggt cgatccggcc
     2761 tgggaagcga aaagcgactg ggaaatctac aaagccatcg cgaagaaatt ctccgaagtg
     2821 tgcgttggtc atctgggtaa agaaactgac atcgtcacgc tgcctatcca gcacgactct
     2881 gccgctgaac tggcgcaacc gctggatgtg aaagactgga aaaaaggcga atgcgatctg
     2941 atcccaggta aaactgcacc gcacattatg gtcgtagagc gcgattatcc ggcaactttc
     3001 gaacgcttta cctctatcgg cccgctgatg gagaaaatcg gtaacggcgg taaagggatt
     3061 gcctggaaca cccagagcga gatggatctg ctgcgtaagc tcaactacac caaagcggaa
     3121 ggtccggcaa aaggtcagcc gatgctcaat accgcaattg atgcggcaga gatgatcctg
     3181 acgctggcac cggaaaccaa tggtcaggta gctgtaaaag cctgggctgc cctgagcgag
     3241 tttaccggtc gtgaccatac gcatctggcg ctgaataaag aagacgagaa gattcgcttc
     3301 cgcgatattc aggcacagcc gcgcaaaatc atctccagcc cgacctggtc tggccttgaa
     3361 gatgaacacg tttcttacaa cgccggttac accaacgttc acgagctgat cccgtggcgt
     3421 acgctctctg gccgtcagca gctgtatcag gatcaccagt ggatgcgtga tttcggtgaa
     3481 agcctgctgg tttatcgtcc gccgatcgac acccgttcgg tgaaagaagt gatgggccag
     3541 aaatccaacg gcaacccgga aaaagcgctc aacttcctga cgccgcacca gaagtggggt
     3601 atccactcca cctacagcga caacctgctg atgctgactt taggtcgcgg tggtccggtg
     3661 gtctggctga gtgaagccga tgccaaagag ctgggtatcg ccgataacga ctggattgaa
     3721 gtcttcaaca gcaacggtgc actgactgcc cgtgcggttg tcagccagcg tgttccggca
     3781 gggatgacca tgatgtacca cgcgcaggaa cgtatcgtta acctgcctgg ttcggaaatt
     3841 acccaacagc gtggtggtat ccataattcg gtcacccgta tcacgccgaa accgacgcat
     3901 atgatcggcg gctatgccca tctggcatac ggctttaact actatggcac cgtaggttct
     3961 aaccgcgatg agtttgttgt agtgcgtaag atgaagaaca ttgactggtt agatggcgaa
     4021 ggcaatgacc aggtacagga gagcgtaaaa tgaaaattcg ttcacaagtc ggcatggtgc
     4081 tgaatctcga taagtgcatc ggctgccaca cctgttcagt tacctgtaaa aacgtctgga
     4141 ccagccgcga aggcgtggaa tacgcgtggt tcaacaacgt ggaaaccaag ccgggccagg
     4201 gcttcccgac tgactgggaa aaccaggaaa aatacaaagg cggctggatc cgtaaaatca
     4261 atggcaaact gcaaccgcgc atgggtaacc gcgccatgct gctgggtaaa atcttcgcta
     4321 acccgcatct gccggggatc gacgattatt acgagccgtt cgattttgat tatcagaacc
     4381 tgcataccgc gccggaaggc agcaaatcgc agccgattgc ccgtccgcgt tcgctgatta
     4441 ccggggaacg gatggcgaaa atcgaaaaag ggccgaactg ggaagatgac ctgggcggtg
     4501 agtttgacaa gctggcgaaa gacaagaact tcgacaacat ccagaaggcg atgtatagcc
     4561 agttcgaaaa caccttcatg atgtatttgc cgcgcctgtg cgaacactgc ctgaacccgg
     4621 catgtgtggc gacctgcccg agcggtgcga tttacaagcg tgaagaagat ggcatcgtcc
     4681 tgatcgacca ggataaatgc cgtggctggc gtatgtgcat caccggatgc ccatacaaaa
     4741 aaatctactt caactggaag agcggtaagt ctgagaagtg catcttctgc tatccgcgta
     4801 ttgaagcggg tcagccgacc gtgtgctctg aaacctgtgt cggtcgtatc cgttatctcg
     4861 gcgtgctgtt gtacgatgcc gacgccattg aacgtgcagc cagcaccgag aacgagaaag
     4921 atctctacca gcgtcagctg gaggtgttcc tcgatccgaa cgatccgaaa gtcatcgagc
     4981 aggcaattaa agacggtatt ccgctgagcg ttattgaagc cgcacagcag tcgccggttt
     5041 ataaaatggc gatggaatgg aaactggcgc tgccgctgca tccagaatat cgcacactgc
     5101 cgatggtctg gtacgtgccg cctctgtctc cgattcagtc tgcagcagac gcgggtgagc
     5161 tgggcagcaa cggcattctg ccagacgtcg aaagcctgcg tattccggta cagtatctgg
     5221 cgaatctgct gaccgccggt gataccaaac cggtactgcg cgcgctgaaa cgtatgctgg
     5281 cgatgcgtca ttacaaacgt gctgaaaccg ttgacggtaa agtcgatacc cgtgcgctgg
     5341 aagaggtcgg tctgaccgaa gcccaggcac aggagatgta ccgttatctg gcgattgcta
     5401 actacgaaga tcgctttgtg gtgccgagca gtcatcgtga actggcgcgg gaagccttcc
     5461 cggagaaaaa tggctgcggc tttacctttg gtgatggctg ccacggttca gataccaaat
     5521 tcaatctgtt caacagccgc cgtatcgatg ccatcgacgt aaccagcaaa acggagccgc
     5581 acccatgatc gaactcgtga ttgtatcgcg tctccttgaa tatccggatg ctgctttatg
     5641 gcagcatcaa caagagatgt ttgaggcaat tgctgcgtcg aaaaatctgt cgaaagaaga
     5701 tgcccatgcg ctgggcattt tcctgcgcga tttaacgacg atggatccgc tcgatgccca
     5761 ggcgcagtac agcgaactgt tcgatcgtgg tcgcgccacg tcgctgttgc tgtttgaaca
     5821 cgttcacggg gaatcccgcg atcgcggtca ggcgatggtg gacctgctgg cacagtacga
     5881 gcagcacggt ttgcagttaa acagccgcga attgcccgac catttgccgc tgtatctgga
     5941 gtatctggcg cagctgccgc aaagcgaagc cgtggaaggt ttgaaagata tcgcgccgat
     6001 tctggcattg ctctgcgcgc gtctgcaaca gcgtgaaagc cgttatgccg tgctgtttga
     6061 tctgctgctg aaactggcga ataccgcaat cgacagcgac aaagtggcgg aaaaaattgc
     6121 cgacgaagcg cgcgacgata cgccgcaggc gctggatgct gtctgggaag aagagcaggt
     6181 taaattcttt gctgacaaag gctgcggtga ttcagcaatc actgctcatc agcgtcgctt
     6241 tgccggtgcc gtcgcgccgc aatatctgaa tatcaccacc ggaggacagc actaatgcaa
     6301 ttcctgaata tgttcttctt tgatatctac ccgtacatag ccggggcggt cttcctgatt
     6361 ggtagctggc tgcgttatga ctacgggcag tacacctggc gcgcggcgtc cagccagatg
     6421 ctggatcgca aagggatgaa cctggcgtcg aacctgttcc atatcggtat tctggggatt
     6481 tttgtcggtc acttctttgg tatgctgacg ccgcactgga tgtatgaagc ctggctgccg
     6541 attgaagtga aacagaaaat ggcgatgttt gctggtggtg ccagcggcgt gctgtgtctg
     6601 attggcggcg tgctgttgct gaaacgtcgt ctgttcagcc cacgcgtgcg tgcaacgact
     6661 accggagcgg atatcctgat cctgtctctg ctggttatcc agtgcgctct gggcttgctg
     6721 accattccgt tctccgccca gcatatggac ggtagcgaga tgatgaaact ggttggctgg
     6781 gcgcagtcgg tagtgacctt ccacggtggc gcttctcaac accttgatgg tgtggcgttt
     6841 atcttccgtc tgcacctggt gctggggatg acgttattct tgctgttccc gttctcacgt
     6901 ctggtgcaca tctggagcgt accggtggag tatctgacac gtaagtatca gctggtgcgc
     6961 gctcgtcact aagcgaattt tagtttgcat agaccccgct tcggcggggt ttttttatgg
     7021 gcacggtacg gggtgagttg tcggatgcgc ttcgcttatc cgacctacag gggaggatat
     7081 tgtaggtccg gtaaacgcgg tgccattggg tagaattagc tcggagtaac aggttttgag
     7141 tatttacagg aagtgatggt ggtgggggaa ggattactca gcgctgcgcg cttcaccctt
     7201 cgggtcgttg cctgcggcaa cgctttctcg ctggcgctcg agtcgcacct tggtcgaagc
     7261 ttctcatcct tccccgcatg ggcagaatat ttaattgcgg attcgtttga gagttcacgg
     7321 acttttgaaa agtgatggtg gtgggagaag gattactcag cgctgcgcgc ttcgcccttc
     7381 gggtcgttgc ctgcggcaac gctctctcgc tggcgctcaa gtcgaacctt ggttgaagct
     7441 tctcatcctt ccccgcatgg gcagaatatt tgattgcgga ttcgtttgag aattccgggg
     7501 cttttgaaag ttatggtggt gggggaagga ttactcagcg ctgcgcgctt cgcccttcgg
     7561 gtcgttgcct gcggccacgc tctctcgctg gcgctcgagt cgaaccttgg tcgaagcttc
     7621 tcatccttcc ccgcatgggc agaatattta atcgcggatt cgtttgagag ttcacggact
     7681 tttgaaagtg atggtggtgg gggaaggatt cgaaccttcg aagtcgatga cggcagattt
     7741 acagtctgct ccctttggcc gctcgggaac cccaccaggg gtaattcaaa ttttgaggta
     7801 atgcttgaga tggtggtggg ggaaggattc gaaccttcga agtcgatgac ggcagattta
     7861 cagtctgctc cctttggccg ctcgggaacc ccaccacggg gtaatgcttt tactggcctg
     7921 ctcccttatc gggaagcggg gcgcatcata tcaaatgacg cgccgctgta aagtgttacg
     7981 ttgagaaaaa tgaactggtt gggtaatttt catccgtaac ggattaaagg taaccagtta
     8041 tttttgctgg cgattaaaga ataatcgtcc gattaccgta aacaaagacg cgctgcgcca
     8101 gtactttgta gagtgcgcga cttaagacgt ttttctcgac gtcacgacct gcgcgcatca
     8161 tatcttcagc tgtgtaggta tgatcgacat gaataacgtc ctgcatgatg attggacctt
     8221 cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat aatcttcacg ccacgttcat
     8281 aggcctggtg ataaggacgt gcgccgataa acgctggcag gaatgaatgg tgaatattga
     8341 tgatcttatt cgggaagcgt gccacaaatt ctggcgttaa tacccgcata tacttcgcca
     8401 gcaccacgta gtcaggctga taagcatcaa tggcatccgc catcttttga tcgtgctcgt
     8461 tgcggcttaa cccttcatgg cttaccagct caaacggaat atcaaaacgc tcaaccagag
     8521 aacgtaaagt atcgtggtta ccaataactg ccgcgatttc gacatccagg ccgccgtaat
     8581 tggctttcat caacaaatcg ccgaggcaat gcgcctcttt agtgaccaga atcactatcc
     8641 ggcgacgacc ggcaggattc agctcacgta cggagccttc tggcaatgcg ctgtcgagat
     8701 ccgccagcag ggtggaatca ttaaaaattc cttccagttc cgtgcgcata aaaaagcgcc
     8761 cggtacggtg atcaacaaat tcattgttct gtacgatatt taactcgtgc ttgtagcaaa
     8821 tattggtaat acgtgcgatc agaccttttt ggtccggaca aatagtacgc agaactttac
     8881 gttggagtga atgcattgct ggaaaaacct tgttgagagt gtttgctaaa ccgtaccgtc
     8941 aaccattatt ggccgcagca ctttttaaat tttttacctg aaccacaagg gcagggatcg
     9001 ttgcggccaa actgcggacg tgtaccgtca atatagtacc attgaccgtt ttctttcaaa
     9061 aatcgcgaac gttcaatgat tgcacctgtt ttgccacctt cggtatatcg cgcaacaaaa
     9121 ctgacaaatc cgatattgtc gacgtcctgc caacaatgtt caaatacggt caatcctaac
     9181 cattcagtat gcgcaaatcc ggcaatcaat tcggcacgta acgccgccgc tccacaagat
     9241 ggatgccagg tctttattaa ataatctgcg tctttcatca caaaagcgca gtaacgcgaa
     9301 cgcatgagat gttctggatc aggtgcaacc ttttcaccag acacataagg gtggcaacat
     9361 aggctatact cgacagcact accacaggga caaagctgag acacaaataa tctccctgga
     9421 aacaataacg gcgtattaac cgcctgagta gcactatgtt aaccgagcag tagcgatgtt
     9481 gctacgattg cattccaggg gaatcttgcg ggaataatga gaaagataaa aatagggctg
     9541 gcgctgggat ctggcgcggc gagaggttgg tcgcatattg gcgttattaa tgcgctaaaa
     9601 aaagtgggta ttgaaattga tatcgttgca ggatgttcaa ttggttcgct ggtgggcgct
     9661 gcctatgcat gcgatcgact atctgcgctg gaagattggg tgacctcttt cagttattgg
     9721 gatgttttac gcctgatgga tctctcctgg cagcgcggtg ggttactgcg cggcgagcgt
     9781 gtcttcaatc aatatcgcga aataatgccg gaaacagaga tcgaaaattg ttcccgtcgc
     9841 ttcgcggctg ttgccaccaa tttaagtact gggcgcgaat tatggtttac tgaaggcgat
     9901 ctccatcttg ctattcgtgc atcatgcagt attccaggac taatggcacc cgttgcgcat
     9961 aacggctact ggctggttga tggcgctgta gttaacccaa ttcctatttc gctcacgcgt
    10021 gcattgggtg ctgatattgt gatagcggtc gacctgcagc acgatgctca tttgatgcaa
    10081 caagatctgc tctcctttaa tgttagtgaa gaaaatagcg agaatggtga ttctctgccg
    10141 tggcatgcgc gtctgaaaga aaggttgggc agcataacga cacgtcgggc ggtgacagcg
    10201 ccaacggcaa cagagattat gaccacttct atccaagtgc tggagaaccg ccttaaaagg
    10261 aaccgcatgg caggtgatcc gcccgatatt ctgattcaac ctgtttgccc gcaaatatct
    10321 acgcttgatt tccatcgcgc gcacgctgcc attgcggctg ggcagctggc agtggaaaag
    10381 aaaatggatg aacttttgcc gttggtacgc accaacattt gaccagaatt tttatctaca
    10441 cttaagttaa ttctgacagg cgcaggtggc aatagcatgc cactattgag taaagccagt
    10501 caggggagag aacatgacgc agccattggt cggaaaacag attctcattg ttgaagatga
    10561 gcaggtattt cgctcgcttc tggattcgtg gttttcctca ttgggagcga caacggtact
    10621 ggcggctgat ggggtggatg cccttgagtt gctgggaggt ttcactccag acctgatgat
    10681 atgtgatatc gcgatgccac gaatgaacgg gcttaaactg ctggagcata tacgtaacag
    10741 aggcgaccag acacctgttc tggtgatatc tgccactgaa aatatggcag atattgccaa
    10801 agcgttacgt ctgggcgttg aagatgtttt gctgaaacca gttaaagatc tgaatcgctt
    10861 gcgcgagatg gtttttgcct gtctctatcc cagcatgttt aattcccgcg ttgaggaaga
    10921 ggagaggctt tttcgcgact gggatgcaat ggttgataac cctgccgcag cggcgaaatt
    10981 attacaggaa ttgcaaccgc cggttcagca ggtgatttcc cattgccggg ttaattatcg
    11041 tcaattggtt gccgcggaca aacccggcct ggtgcttgat attgccgcac tttcggaaaa
    11101 cgatctggca ttttattgcc ttgatgtcac ccgagctgga cataatggcg tacttgctgc
    11161 cttgttatta cgcgcattgt ttaacggatt attacaggaa cagcttgcac atcaaaatca
    11221 acggttgcca gagttgggcg cgttattgaa gcaggtaaac catttactcc gtcaggccaa
    11281 tctaccgggg cagtttccgc tattagttgg ctattatcat cgcgaactga aaaatctcat
    11341 tctggtttct gcgggtctga atgcgacatt aaatactggc gaacaccagg tgcaaatcag
    11401 taatggtgtt ccgttaggca ctttaggtaa cgcttatttg aatcaattga gccagcgatg
    11461 cgatgcctgg caatgccaaa tatggggaac cggtggtcgg cttcgcttga tgttgtctgc
    11521 agaatgagca aacgataacg cgggctaaat ttgcattacc tgctaatgtc ggctggtgtt
    11581 actatcgtcg ccattcgtat aagtaattgt cttaattatg ctaactcgcc tccttttcag
    11641 aacttagccc cttcggggtg ctgatatact gggatgcgat acagaaatat gaacacgttc
    11701 aaaacacgaa cagtccagga gaatttaaat ggctgccatt aatacgaaag tcaaaaaagc
    11761 cgttatcccc gttgcgggat taggaaccag gatgttgccg gcgacgaaag ccatcccgaa
    11821 agagatgctg ccacttgtcg ataagccatt aattcaatac gtcgtgaatg aatgtattgc
    11881 ggctggcatt actgaaattg tgctggttac tcactcatct aaaaactcga ttgaaaacca
    11941 ctttgatacc agttttgaac tggaagcaat gctggaaaaa cgtgtaaaac gtcaactgct
    12001 tgatgaagtg cagtctattt gtccgccgca cgtgactatt atgcaagttc gtcaggggct
    12061 ggcgaaaggc ctgggacatg cggtattgtg tgctcacccg gtagtgggtg atgaacctgt
    12121 ggctgttatt ttgcctgacg ttattctgga tgaatatgaa tccgatttgt cacaggataa
    12181 cctggcagag atgatccgcc gctttgatga aacgggtcat agccagatca tggttgaacc
    12241 ggttgctgat gttaccgcat atggcgttgt ggattgcaaa ggcgttgaat tagcgccggg
    12301 tgaaagcgta ccgatggttg gtgtggtaga aaaaccgaaa gcggatgttg cgccgtctaa
    12361 tctcgctatt gtgggtcgtt acgtacttag cgcggatatt tggccgttgc tggcaaaaac
    12421 ccctccggga gctggtgatg aaattcagct caccgacgca attgatatgc tgatcgaaaa
    12481 agaaacggtg gaagcctatc atatgaaagg gaagagccat gactgcggta ataaattagg
    12541 ttacatgcag gccttcgttg aatacggtat tcgtcataac acccttggca cggaatttaa
    12601 agcctggctt gaagaagaga tgggcattaa gaagtaacat ccgtatcggt gttatccacg
    12661 aaacggcgtt gagcaatcga cgccgttttt ttatagctta ttcttattaa attgtcttaa
    12721 accggacaat aaaaaatccc gccgatggcg ggatttttaa gcaagtgcaa tctacaaaag
    12781 attattgctt gatcaggaaa tcgtcgaggg atttaccttg ctcatccatt gcttttttga
    12841 ttacagctgg agtacggccc tggccagtcc aggttttagt ttcgccgttt tcgtcaacgt
    12901 agctatattt tgccggacgc tgagcacgtt tagctttggt gccagattta acggcagcaa
    12961 ggctattcag cagttcgttc gggtcaatac cgtcagcgat cagcatttcg cgatattgct
    13021 gcagtttacg agtgcgctct tcaacttcag cagcagccgc gctttcttct tcgcgacgtt
    13081 cgttaacaac aacttctaat ttttccagca tttcttccag cgtttcaagt gtacattctc
    13141 ttgcctgcgc acgaagagta cggatgttgt tcagaatttt aagtgcttcg ctcattgtag
    13201 taatctcaaa cttatattgg ggtggtttgt tgaggtaata atagagcctt aaattcagtt
    13261 gtgcaatagc caggaatgta aggaattcaa aattgttctt tattttgtgc cgccaataaa
    13321 tatcttttca taaaattagc cagaaaagac gcggcatata gccctatttg cgccgatgat
    13381 ttcgcagcac gtgaggttaa aacttcctga ttcatgtcac attttatggg gagattatcg
    13441 taggctgacg acctttcagt cttctgtatt agtcgtgttt acgagaattc cctattaagc
    13501 gaatgatgaa aagtagaaca gtcgcaataa gagcagggac ttagtattgc actatctcca
    13561 ggaggtcaac agagggctat tacttgcgca acaggttaaa gattgtgaat agttaccagc
    13621 agtcatttac ccgcttataa caagcgaggc agttgtaatg atagctcaga aggattatgc
    13681 aaggcttcgt aagggagaac gcatatacca cttctgtgca tactgttgag ctgaaaaact
    13741 gacgaattat gataaactcc agccaacttt aattcatatc attgagggcc tgtggctgat
    13801 ggcacagcta tatttctact attccgcaat gaatgcgggt aagtctacag cattgttgca
    13861 atcttcatac aattaccagg aacgcggcat gcgcactgtc gtatatacgg cagaaattga
    13921 tgatcgcttt ggtgccggga aagtcagctc gcgtataggt ttgtcatcgc ctgcaaaatt
    13981 atttaaccaa aattcatcat tatttgatga gattcgtgcg gaacatgaac agcaggcaat
    14041 tcattgcgta ctggttgatg aatgccagtt tttaaccaga caacaagtat atgaattatc
    14101 ggaggttgtc gatcaactcg atatacccgt actttgttat ggtttacgta ccgattttcg
    14161 aggtgagtta tttattggca gccaatactt actggcatgg tccgacaaac tggttgaatt
    14221 aaaaaccatc tgtttttgtg gccgcaaagc aagcatggtg ctgcgtcttg atcaagcagg
    14281 cagaccttat aacgaaggtg agcaggtggt tattggtggt aatgaacgct acgtttctgt
    14341 atgccgtaaa cactataaag aagcgttaga agtcggctca ttaacggcta ttcaggaaag
    14401 gcatcgccac gattaataag aatttcttta ctgacagggt gagcagggcg cttttatcct
    14461 gtcagttcgt tttactcact tcatccggtg tatataccct tctcgacagt tctttaacgc
    14521 cactataccc ctcacagagc gcttttacag tgacattgac ttgtgacctg taaagtacaa
    14581 tatcgctgtg tgtaggcgtt atacatcgcc gtaaatatga tgaaggcaaa tgctgtcggt
    14641 ttatgggaaa gttgctttgg gtaaaacaaa aaaacggccc cagaagggac cgtttatgtt
    14701 gccagacagc gctactgatt aagcggattt cttcgctttt ttctcagctt tagccggagc
    14761 ggcttctttt ttcgctgcag tttcaccttc tacataatca cgaccgtagt aggtatccag
    14821 cagaatctgt ttcagctcgg agatcagtgg gtaacgcggg ttagcgccgg tgcactggtc
    14881 atcgaatgca tcttcagaca gtttatccac attcgccagg aagtctgctt cctgaacgcc
    14941 agcttcacgg atagatttcg gaatacccag ttcagctttc agcgtttcca gccatgccag
    15001 cagtttctcg atcttagcag cagtacggtc gcccggtgcg ctcagaccca ggtggtcagc
    15061 aatttcagca taacgacggc gagcttgcgg acggtcatac tggctgaatg cagtctgctt
    15121 ggtcgggttg tcgttcgcgt tgtagcgaat aacgttacaa atcagcaggg cgtttgccag
    15181 tccgtgcgga atatggaact gggaacccag tttgtgcgcc attgagtgac atacacccag
    15241 gaaggcgttc gcaaacgcga tacccgcgat agttgctgca ctgtgaacac gttcacgcgc
    15301 taccggattt ttagaccctt cgtggtagga cgctggcaga tattctttca gcagtttcag
    15361 tgcctgcaga gcctgaccat cagagaactc agatgccagt acagaaacat aagcttccat
    15421 ggcgtgagtt actgcgtcca gaccaccgaa agcacacagg gacttcggca tgtccataac
    15481 caggttggca tcgacaatcg ccatatccgg ggtcagcgca tagtctgcca gcggatattt
    15541 ctgaccagta gcgtcgtcag ttacaaccgc aaacggagtg acttcagaac ctgtaccaga
    15601 agtggtggtg acagcgatca ttttcgcttt cacgcccatt ttcgggaact tgtagatacg
    15661 tttacggata tccataaagc gcagcgccag ttcttcgaag tgagtttccg gatgttcgta
    15721 catgacccac atgatcttcg cggcgtccat cggggaacca ccacccagcg cgataatcac
    15781 gtctggtttg aaggagtttg ccagttctgc acctttacga acgatgctca gggtcgggtc
    15841 agcttctact tcgaagaaga cttcagtttc aacgcctgct gctttcagta cggaagtgat
    15901 ctgatcagca taaccattgt tgaacaggaa gcggtcagtc acgatgagcg cacgtttgtg
    15961 gccatcagta atcacttcat ccagcgcgat tggcagggag ccacggcgga agtagataga
    16021 tttcggaagt ttgtgccaca acatgttttc agctcgctta gcaacggttt tcttgttgat
    16081 caggtgtttc ggaccaacgt tttcagagat ggagttacca ccccaggaac cacaacccag
    16141 agtcagggaa ggtgcgagtt tgaagttata caggtcaccg ataccaccct gagaagccgg
    16201 ggtgttaatc aggatacgag ccgttttcat tttctgaccg aagtaagaaa cgcgagccgg
    16261 ttggttatcc tggtcagtgt acaggcaaga ggtatgaccg ataccgccca tagctaccag
    16321 tttctctgct ttttctaccg cgtcttcgaa atctttagcg cggtacattg ccagagtcgg
    16381 ggacagtttt tcatgtgcga acggttcgct ttcatcaaca acggtcactt caccgatcag
    16441 aatcttggtg ttttctggta cagagaagcc tgccagttca gcaattttat aggctggctg
    16501 accaacgata gccgcgttca gcgcaccgtt tttcaggata acgtcctgaa cagctttcag
    16561 ctctttaccc tgcaacagat agccgccatg ggttgcgaaa cgttcacgta ctgcgtcata
    16621 aacagagtca acaacaacaa cagactgttc agaagcacag attacgccgt tgtcgaaggt
    16681 tttggacatc agcacagatg caactgcacg tttgatatca gcagtttcat cgataacaac
    16741 aggcgtgtta cccgcgccta caccgatagc tggtttaccg gagctgtatg cggctttaac
    16801 catgcccgga ccaccagtcg cgaggatcag gttgatgtct gggtggtgca tcagtgcgtt
    16861 agacagttca accgaaggtt gatcgatcca gccgatcaga tctttcggag caccggcagc
    16921 gatagcagcc tgcagaacga tatcagctgc tttgttggtt gcatcttttg cacgtgggtg
    16981 cggggagaag ataatggcgt tacgggtctt caggctgatc agcgatttga agatagcagt
    17041 tgaagtcggg ttagtggtcg gaacgatacc gcaaataata ccgattggtt cagcgatagt
    17101 gatggtacca aaagtgtcgt cttcagacag aacaccacag gttttttcat ctttataggc
    17161 gttgtagata tattcagaag caaagtggtt tttgatcact ttatcttcga cgatacccat
    17221 gccggattcg gcaacggcca ttttcgcgag tgggattcga gcatctgcgg cagccagagc
    17281 ggcggcgcgg aagattttgt ctacttgctc ttgagtgaaa ctggcatatt cacgctgggc
    17341 tttttttaca cgctctacga gtgcgttaag ttcagcgaca ttagtaacag ccataatgct
    17401 ctcctgataa tgttaaactt ttttagtaaa tcatctgctc gaatacgaga gtatagtcag
    17461 tgcggtgatg atttgcttaa ccgatgaaaa tcaaaagctt actcgcgctc acactcactg
    17521 tgatttacta aaagagttta aacattagag ttattatctc taatgcgtca cttccaggtg
    17581 gcgtaagcaa gattactcac ttctgggtac tgattacgtg atccaaatca aatttttgca
    17641 aagctgacac ctttcagcat cgcttttcgc cattatagct aacagttaat aaattgtagt
    17701 atgatttgtt ggctacatta tcatgttttg cacaaccaga taacaataac gaatgatagc
    17761 aattttaagt agttaggagg tgaaaaatgc tgtcaaaagg cgtattgtca gcgcgtcttt
    17821 ttaaccttat ttatggctaa cattatccgg cttttgcttc ggagctaacc gtgattcaga
    17881 ccctttttga ttttcccgtt tacttcaaat ttttcatcgg gttatttgcg ctggtcaacc
    17941 cggtagggat tattcccgtc tttatcagca tgaccagtta tcagacagcg gcagcgcgaa
    18001 acaaaactaa ccttacagcc aacctgtctg tggccattat cttgtggatc tcgctttttc
    18061 tcggcgacac gattctgcaa ctttttggta tatcaattga ttcgttccgt atcgccgggg
    18121 gcatcctggt ggtgacgata gcgatgtcga tgatcagcgg caagcttggc gaggataaac
    18181 agaacaagca agaaaaatca gaaaccgcgg tacgtgaaag cattggtgtg gtgccactgg
    18241 cgttgccgtt gatggcgggg ccaggggcga tcagttctac catcgtctgg ggtacgcgtt
    18301 atcacagcat tagctatctg tttggtttct ttgtggctat tgcattgttc gctttatgtt
    18361 gctggggatt gttccgcatg gcaccgtggc tggtacgggt tttacgccaa accggcatca
    18421 acgtgatcac gcgtattatg gggctattgc tgatggcatt ggggattgaa tttatcgtta
    18481 ctggtattaa ggggattttc cccggcctgc ttaattaatt cctttcaaat gaaacggagc
    18541 tgtcatgctc cgtttacttc gtcattattt ttactttgtt cccgcgcagt tatcaaaagc
    18601 aaaaggaatg ggtgaaaata ttcttctcaa attacagttg gttataagga tttccttaac
    18661 tgcttctcct caccatcatg ttattttcgc cacatcataa tcctgggctt gctgaagaat
    18721 aattgaaatg atattagtaa ttccactgcc tttggtagag gaaagtgcta aataataatc
    18781 aattgttaaa ttattgtgca tttcactact ggaactgtaa tcagaaaaga gagacatgct
    18841 taaccaatct ctatttgatt gaattaaaag atgtttgtta agacatggat gcaagctata
    18901 gattctgata cggtcaataa aagagaattg cttaacaatt ttgcaaaatg tattggcgag
    18961 taagaaccgc atttggtact ttccgggcaa ccgccagacg attctttatt ggtaatgaga
    19021 ataattaaca attaaagagc gtcgcgaaag aataatgtgt ctcgacaggg gagacacagt
    19081 acgaatcgac ataaggtgat cgtctgaatc accagaataa ataaagtcgg tgatagtaat
    19141 acgtaacgat aaagtaacct gacagcagaa agtctccgag cctgtgcagg gtcccaatcc
    19201 gggattacac atgctggtta ataccagtaa ttataatgag ggagtccaaa aaacaatgac
    19261 caacatcacc aagagaagtt tagtagcagc tggcgttctg gctgcgctta tggcagggaa
    19321 tgtcgcgctg gcagctgatg tacccgcagg cgtcacactg gcggaaaaac aaacgctggt
    19381 acgtaacaat ggttcagaag ttcagtcatt agatccgcac aaaattgaag gtgttccgga
    19441 gtctaatatc agccgagacc tgtttgaagg cttactggtc agcgatcttg acggccatcc
    19501 ggcacctggc gtcgctgaat cctgggataa taaagacgcg aaagtctgga ccttccattt
    19561 gcgtaaagat gcgaaatggt ctgatggcac gccagtcaca gcccaagact ttgtgtatag
    19621 ctggcaacgt tctgttgatc cgaacaccgc ttctccgtat gccagttatc tgcaatatgg
    19681 gcatatcgcc ggtattgatg aaattcttga agggaaaaaa ccgattaccg atctcggcgt
    19741 gaaagctatt gatgatcaca cattagaagt caccttaagt gaacccgttc cgtacttcta
    19801 taaattactt gttcacccgt caacttcacc ggtgccaaaa gccgctatcg agaaattcgg
    19861 cgaaaaatgg acccagcctg gtaatatcgt caccaacggt gcctatacct taaaagattg
    19921 ggtcgtaaac gaacgaatcg ttcttgaacg cagcccgacc tactggaaca acgcgaaaac
    19981 cgttattaac caggtaacct atttgcctat tgcttctgaa gttaccgatg tcaatcgcta
    20041 ccgtagtggt gaaatcgaca tgacttataa caacatgccg atcgaattgt tccagaagct
    20101 gaaaaaagag atcccggacg aagttcacgt tgatccatac ctgtgcactt attattacga
    20161 aattaacaac cagaaaccgc catttaacga tgtacgtgtg cgtaccgcac tgaaactggg
    20221 tatggaccgc gatatcattg ttaataaagt gaaagcgcag ggcgacatgc ccgcctatgg
    20281 ttacactcca ccgtatactg atggcgcaaa attgactcag ccggaatggt ttggctggag
    20341 ccaggaaaaa cgtaacgaag aagcaaaaaa actgctggct gaagcgggtt ataccgcaga
    20401 caaaccgttg accatcaacc tgttgtataa cacctccgat ctgcataaaa agctggcgat
    20461 tgcagcctct tcattgtgga agaaaaacat tggtgtaaac gtcaaactgg ttaaccagga
    20521 gtggaaaacg ttcctcgaca cccgtcacca gggcactttt gatgtggccc gtgcaggctg
    20581 gtgtgcagac tacaacgaac caacttcctt cctgaacacc atgctgtcga acagctcgat
    20641 gaataccgcg cattataaga gcccggcctt tgacagcatt atggcggaaa cgctgaaagc
    20701 gactgacgag gcgcagcgca cagctctgta cactaaagca gaacaacagc tggataagga
    20761 ttcggccatt gttcctgttt attactacgt gaatgcgcgt ctggtgaaac cgtgggttgg
    20821 tggctatacc ggcaaagatc cgctggataa tacctacacc cggaatatgt acattgtgaa
    20881 gcactaatgg caatacgtgg ggcaggcgtg tcctgctcca cggtgtctga tttttatcgc
    20941 attacagaag gcacaggcca gaaggtaggg caatgttaaa atttattcta cgtcgctgtc
    21001 tggaagcgat tccgacgcta tttattctta ttactatttc gttcttcatg atgcgcctcg
    21061 cgccgggaag cccttttacc ggcgaacgta ctttaccgcc agaagtgatg gccaatatcg
    21121 aagcgaaata tcatcttaat gatccgatca tgacacagta tttcagctac ctgaaacaac
    21181 tggcgcatgg cgatttcggt ccatcgttta aatataaaga ttattcggtc aacgacctgg
    21241 ttgcatccag ttttcccgtt tctgccaaac tgggagccgc agcatttttc cttgcggtaa
    21301 tactgggtgt tagtgctggc gttattgccg cattaaaaca aaacaccaaa tgggactata
    21361 ccgtgatggg gctggcaatg accggggttg ttatccctag ttttgtggtt gcgccattat
    21421 tagtcatgat atttgcgatc attttgcatt ggctgccggg cggtggctgg aatggtgggg
    21481 cgcttaaatt catgatattg ccgatggtgg cgttgtcact cgcctatatc gccagtattg
    21541 cgcgtattac ccgtggctct atgattgagg ttttacactc caactttatt cgtactgccc
    21601 gggcgaaagg gttacctatg cggcggatca ttttacgcca cgcattaaaa cctgctctgt
    21661 tacccgtgct ctcctatatg ggccccgcat ttgtcggcat tattaccggt tctatggtta
    21721 tcgaaaccat ttatggtttg ccggggattg ggcaactgtt cgttaatggc gcattgaacc
    21781 gtgactattc cttagtgtta agcctgacta tcctggttgg tgcgttaacc attttgttta
    21841 atgccattgt cgatgtgcta tatgcggtta tcgacccgaa aatccgttac tgatactgga
    21901 gctcgcgatg atgttaagta agaaaaacag cgagacgctg gaaaatttca gtgaaaagct
    21961 ggaggtcgaa gggcgcagct tgtggcagga cgcacgtcga cgttttatgc ataaccgtgc
    22021 ggcggttgcc agtctgatag tgctggtgct gatcgcgtta tttgtaatcc tggcaccgat
    22081 gctttcgcag tttgcctatg acgatactga ctgggcgatg atgtccagcg ccccggatat
    22141 ggagtccggt cactactttg gtactgactc atccggtcgc gacctgcttg tacgcgttgc
    22201 gattggcggg cgtatctcac tcatggtcgg tgttgctgcg gcactggtgg ctgtggtcgt
    22261 cgggacactt tatggttcgc tttccggtta tctgggcggt aaagtggatt cggtaatgat
    22321 gcgtctgctg gaaatcctca actccttccc attcatgttc ttcgtcattt tgctggtgac
    22381 ctttttcggt caaaacatcc tgctgatttt cgtggcgata ggcatggttt cctggctgga
    22441 tatggctcgt attgtgcgtg ggcaaaccct gagtctgaag cgcaaagagt ttattgaggc
    22501 ggcacaagtt ggcggtgtat cgacgtcggg cattgttatt cgccacattg tgccgaatgt
    22561 actcggtgtg gtggtggtct acgcatcact tctggtgccc agcatgatcc tctttgaatc
    22621 tttccttagc ttcctggggt tgggtacgca agagccgtta agcagctggg gtgcattgct
    22681 gagtgatggc gcgaactcga tggaagtctc tccatggtta ctgttgttcc cagcgggatt
    22741 cctcgtggtg acactgtttt gtttcaactt tatcggcgat ggcttgcgtg atgccctcga
    22801 cccgaaagat cgttaaggag tgcagccatg agcgtaattg aaactgcaac tgtgccgctc
    22861 gcacaacaac aggctgacgc actgctgaac gtgaaagatt tgcgtgtcac ctttagtacc
    22921 ccggacggcg acgtcacggc ggtcaatgat ttgaattttt ccctacgtgc cggagaaacg
    22981 ctgggcattg taggtgagtc tggttcgggt aaatcgcaaa ctgcatttgc gttgatgggc
    23041 ctgttggctg ccaacggacg tattggcgga tcggcaacct tcaatgggcg tgaaatcctc
    23101 aatttgccag agcatgaact caataaactg cgcgctgaac aaatctcaat gatttttcag
    23161 gacccaatga cttcgttgaa tccctatatg cgtgtcggtg agcagttgat ggaagtgctg
    23221 atgctgcata agaacatgag caaagctgag gcgtttgaag agtcggtgcg gatgctcgat
    23281 gcggtaaaaa tgccggaagc gcgtaaacgc atgaaaatgt acccgcacga attttctggc
    23341 ggcatgcgtc agcgagtcat gattgcgatg gcattgctat gtcgacctaa gctgctgatt
    23401 gcggatgaac ccactactgc gctggacgtc accgtacagg cacagatcat gacgctatta
    23461 aatgaactga agcgggaatt taataccgcc atcattatga tcacccacga tcttggcgtg
    23521 gtggcgggga tctgcgacaa agtgctggta atgtacgccg ggcgcacgat ggaatatggc
    23581 aatgcgcgcg atgtctttta tcaacccgtt catccttatt ctatcggttt gctcaacgcg
    23641 gtgccgcgtc ttgatgcgga aggtgaaaca atgttgacca tcccgggtaa tccgccaaac
    23701 ttgctgcgat taccgaaagg ttgcccgttc cagccacgtt gtccgcatgc gatggaaatt
    23761 tgtagtagcg caccgccgct ggaagagttt acgcctggcc gtctgcgtgc ttgctttaaa
    23821 ccggtggagg aactgttatg aatgctgtaa ctgaaggaag aaaagtcctc cttgaaatcg
    23881 ccgatctgaa agtgcacttt gaaatcaaag atggcaaaca gtggttctgg caaccgccga
    23941 aaacgctcaa agccgtcgat ggtgtcactc ttcgcctgta tgaaggggaa actttaggtg
    24001 tggtagggga atcgggatgc ggtaagtcca cctttgctcg cgccatcatc ggtttggtca
    24061 aggcgaccga cggtcatgtt gcctggttag gtaaagagtt gctgggcatg aagcccgatg
    24121 aatggcgtgc cgttcgcagt gatattcaga tgattttcca ggatccgttg gcatcgctta
    24181 acccgcgtat gaccatcggc gagatcatcg ctgaaccact gcgtacttat catccgaaaa
    24241 tgtcacgcca ggaagttcgc gagcgcgtga aggcgatgat gctgaaagtc gggttattgc
    24301 ctaacctgat taaccgctat ccgcatgagt tctctggtgg gcagtgccag cgtatcggga
    24361 ttgctcgtgc tcttattctt gaaccgaagc tgattatctg tgatgagccg gtgtcggcgc
    24421 tggacgtgtc aattcaggcg caggtggtca acctgctcca gcagctgcaa cgcgagatgg
    24481 gattgtcatt aatttttatc gctcacgacc tggccgtggt aaaacacatt tccgatcgtg
    24541 tgctggtgat gtatctcggc catgcggtag aactggggaa ctatgatgag gtctaccaca
    24601 atccactaca tccttacacc cgggcattga tgtcggcagt ccccatacct gatccggatc
    24661 tggagaagaa caaaaccatc cagttactgg aaggggaatt accgtcgccg atcaacccgc
    24721 cttccggttg tgttttccgt acccgttgcc cgattgccgg tccggagtgc gccaaaacac
    24781 gtcctgttct ggaggggagt ttcagacacg ccgtttcttg cctgaaagtc gatccgcttt
    24841 aaacacaata agggctgaca gttgtcagcc ctttttcacg ccgaaagcga ttatttattc
    24901 ccgccagatg atatggcaca gtttgtgatc tttttcgcgg cataacagaa tgcgggcgaa
    24961 aacatcgttg atttcaccat cttcactgtc cgccagacca atcaccactt cggcaaaaaa
    25021 gtccgggttc aaatcgaaat caacatgttc ctgccagtct tctgccggat cgaataactc
    25081 cgcgccgccg cgctcttcaa actgaagatt gaacagcaga acatcagctg gatcgagatt
    25141 gtcggcagcc agttcgagaa aaatatcgta ggcctgctca agcgtttcat cttcagtcag
    25201 gcgattgttt agatccatat ccatagtcac tacctgttta acctctgttg gcgacgtttt
    25261 acagcaacgg gctgaagaag taaaacagtc gctcggcgac acgttgccat aatggacgtt
    25321 ttagccataa acgggcatcg agcagacgag aacgcgaaat ataatcgtcc tgaacggcgg
    25381 cgaggtcagc accaaaccct ttatcgtcga ttgccagggt aatctcgaaa ttcagccaca
    25441 gactacgcat atcaaggtta acggtaccaa ccagacttaa ttcgccatcg accagcacgc
    25501 tcttggtatg caataacccg ccttcaaact gataaatttt aaccccagca gccagcagtt
    25561 ccgtaaagaa cgcgcgactg gcccagccaa ccagcattga atcatttttt cgcggaagga
    25621 taatactgac atccaccccg cgctgcgccg ccgtgcaaat cgcatgaagt aaatcatcgc
    25681 tgggcacaaa gtagggcgtc gtcatgatca aatattcacg tgccgaataa gccgcagtca
    25741 gtaatgcctg gtgaatgaga tcttccggaa agccggggcc agaagcaatt gtgtgaatgg
    25801 tgtgaccgct ggcctgctca aacggcataa tattgacatc tggtggtggc ggcagaatac
    25861 gtttcccggt ttcaatttcc cagtcgcagg aataaataat ccccatcgcg gtagcgatgg
    25921 gaccttccat acgcgccatc agatcaatcc attgccctac gcccgcatct tgtttgaagt
    25981 agcgagggtc gaccatattc atgctgccgg tgtacgcgat gtaattatcg atcatgatca
    26041 tcttgcgatg ttggcgcagg tccatacggc gtaaaaacac acgcatcaga ttgaccttta
    26101 aggcttcgac cacttcaata ccggcattac gcattagctc gggccagggg ctgcggaaaa
    26161 aagccacact cccggcggag tcgagcatca atcggcagtg aatgccgcgt cgcgcagcgg
    26221 ccattaatga ttcagccacc tggtccgcca tgccgccggg ctgccagata taaaacacca
    26281 tctcaatatt atggcgcgcg agctggatgt cgcggattaa cgcctgcatc acatcatctg
    26341 actcggtcat cagttgtagc tgattccctt tgacccctgc gatcccctga cgacgctcgc
    26401 aaagcttgaa taatggcgca gcgacactgc tattttcttc ggcgaagata tgcttacagg
    26461 ctttaaggtc gttaagccat tttgcggtgg aaggccacat cgctctggcg cgctcagcgc
    26521 ggcgtttgcc taaatggagc tcgccaacgg caagataggc aataattccg actaacggca
    26581 gaatgtaaat aatcaacagc caggccatcg cggagggaac tgcgcgtcgt ttcattagaa
    26641 tgcgtaaagt tacgcctgca atgagcaacc agtatcccag aatggccaac caactcacca
    26701 acgtataaac ggttgtcata gattagaaat ccttttgaaa gcgcattgtt atgagtttac
    26761 gcatatggat tcatctggca aataaaaacg cgggaaaagc actggtcagc gtcgggggcg
    26821 ggggtataat gaccattctg ttattgcata gagtagttaa catgaagcgg agtagaacgg
    26881 aagtggggcg ctggcgcatg cagcgtcagg ctagccgacg taaatcgcgt tggcttgagg
    26941 ggcaatcgcg ccgaaatatg cgtatccaca gcatcaggaa gtgcattcta aacaaacagc
    27001 gtaactcgtt attgtttgcg atctacaata tctaaatgta ctgggcacca ttgcggtgcc
    27061 caataagttt actcttccgt ctttaaactc tatttttgcg ccgattcttt actgtcagct
    27121 ttcgtttctt gttcatcgtt atcgctgaaa atgccatgac cggagttcaa caacattgaa
    27181 acgagcatat cattattaat ctcttcacca tttaaaactc gcgccagaat ttcgctgcca
    27241 aacagttgcg gcgagagaat gatatccgga tgtaccatct taatcttatt caggtttttg
    27301 ctatcactga cggcgagaac tgttttgaca tcactgctca tatcttttgc cgagagtaca
    27361 acaaacgcgt tatctgcatc gttatcactc agcgccagaa tggcccggca acgatcgatt
    27421 cccgctttct tcaagactga actgtcatta ctgtcaccgg ggataacatc agcgttatcg
    27481 cctaagcgtt gctcaagttg cttgatatca tcttcaggca agttgctgat aaccgttacg
    27541 ttttgtccgc gttgattcag ttgcaggatc gtattgatgg cgagaatcga atgtccgcaa
    27601 acaataaaat gatctttacg atgcattgta tgattgtttc cttttacaag tttgttgaat
    27661 cccccgcgga taagcggacc aaaaattgag gtcatagagg tggcaaaaac ggtaatgccg
    27721 gaaataatga ccgaaatagt gaacaatcgt gctgattcag aaacagggac aatatcgcca
    27781 tagccgacgg ttgacatggt ttctatcgaa aaatagaacg cggtcatcaa actttctatt
    27841 cgcggattaa aaccttcgct taaataaagc gcaccgtagg tggagtaaaa cagtaacgtc
    27901 gtgaaactaa taaatgcgaa aattgtcccg gctgcggcgc tactgtggga gaagtctttg
    27961 cgcagtatca gcaaaaacac cagcgtaaaa atgcaaaatc caatactaaa tttcagccag
    28021 ggataaaaat gcagggtgta aattagcgct atcaacaaca aaatgatact gattgcccat
    28081 gcgagctttg cccggaacag taagccaatg gagttcagga caagaaagac gccgagcata
    28141 aagagtgggg cgtttgccag caaggagaga tccaactctg aaaatgcttt gatatggaaa
    28201 atatctagca aattgaccga cataccatag attgttttaa aaataagcaa tccatttaaa
    28261 aagacggcca gcgcgagaat gtcgtgccgt agggtcaccc ataaattagt tgctgtttgt
    28321 ttgaatgtag cccagtgact cactgatacc tctgtaacca caattttgtt taattatctg
    28381 agcgtgcaca ttttctgaac atacatgcag cgcgtgaatg tgttaaacaa tagtagatga
    28441 ctttatccgc agtaatcaaa ggttttgagt gagcaactct gcgtcaggaa agggcatttt
    28501 tcctgaatat gcttacatca tataaagaaa tacagataaa gttattatct gcttgtgggg
    28561 gtgaatgcac tgaccgacct ataaggaaag gccaaacaag aacacggttg caaaaaccgt
    28621 gcccttaaat attgaatctc tattcagaac actttcttaa atggtttcac tgaaacgtgt
    28681 tcatagactc ctgccgctac gtacgggtca gcatcggccc aggcctgagc tgcttccagc
    28741 gattcaaatt cagcaataac ggttgagcca gtaaatcccg cagcccctgg atcgttactg
    28801 tctaccgctg gcattggacc agctgtcaac aaacgacctt catcatgcag taactgtaaa
    28861 cgtgctaaat gtgccggacg aacggaaagg cgtttttcga ggctatcagc tttgtcttga
    28921 gcgtaaataa catacaacac gggcacaact ccttgttcgg gaaagttgta aagtacgtta
    28981 tttgaaaggg cgaagatctg caacggaaag atgatgtctt tgttaagacc atgcataaag
    29041 taaggtcaat tacgccaaaa atgacatttt cactgatcct gatcgtcttg ccttattgaa
    29101 tatgattgct atttgcattt aaaatcgagg cctggttttt ctactgaaat gattatgact
    29161 tcaatgaccc ttgatttacc tcgccgcttc ccctggccga cgttactttc ggtctgcatt
    29221 catggtgctg ttgtggcggg tctgctctat acctcggtac atcaggttat tgaactacct
    29281 gcgcctgcgc agccaatttc tgtcacgatg gttgcgcctg cggatctcga accgccacaa
    29341 gccgttcagc cgccaccgga gccggtggta gagccagaac cggaacctga gccgatcccc
    29401 gaaccgccaa aagaagcacc ggtggtcatt gaaaagccga agccgaaacc taagccaaaa
    29461 ccgaagccgg tgaaaaaggt acaggaacag ccaaaacgcg atgtcaaacc cgtagagtcg
    29521 cgtccggcat caccgtttga aaatacggcg cccgcgcgcc cgacatcaag tacggcgacg
    29581 gctgcaacca gcaagccggt taccagtgtg gcttcaggac cacgcgcatt aagccgtaat
    29641 cagccgcagt atccggcacg agcacaggca ttgcgcattg aagggcaggt taaagttaaa
    29701 tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa tcctctcagc caagcctgcg
    29761 aatatgtttg agcgtgaggt gaaaaatgcg atgcgcagat ggcgttatga gccgggtaag
    29821 ccaggcagtg ggattgtggt gaatatcctg tttaaaatta acggcaccac cgaaattcag
    29881 taagcataaa gtcaaaagcc tccgaccgga ggcttttgat tattactcaa caggtaaggc
    29941 gcgaggtttt ccttcaggat caaccgcgac atacttaaat aatgcttctg tcgctttata
    30001 gcgttgccca atgggttcag acgcgacttt tttcacccac acttcaatat taatgctaac
    30061 cgatgtcgtc cctttctgga cacagcgtgc atagcagcac accacatcgc cgaccgcaac
    30121 cggtcgtaag aaagtcattc cttcaacccg cacggtcact acgcgaccgt gggcaatttc
    30181 tttcgccaga atagcgccgc caatatccat ttgtgacatt aaccaaccac caaagatgtc
    30241 accattggca ttggtatcgg cgggcatggc taaagtacgt aaaacaagat cgccctgagg
    30301 gacgttatgt gttgtagaca tggtaaaacc gacttaaaag gaaaatcaca ggcgcgatgc
    30361 tactatgatt ttcctgagga gaacagagga gatacagcgc cagccccgaa ggactggcag
    30421 tctggttagg atttatcttc ctgcggcatg tggcggtaga tatagatacc gcttaacaat
    30481 gtgaagatta aggtaagggc ggtcaggcca aagactttaa agttgaccca aatattttgc
    30541 ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa ggataaagaa aacagcccag
    30601 gccagattca gcttcgacca taccggttgc ggcagcgtga gttctttacc cagcatccgc
    30661 tgaattagcg gctttttcat cacccattgg ctgactaaca gggcacccgc aaacagggca
    30721 taaatgacag taaccttcca tttaataaac tcatcattgt ggaagaacag ggtcaagcca
    30781 ccgaagacga ccaccagaac aaaagtgatc agggccatct tctcaacctt acgaaagcga
    30841 acccagctat atataagcac aagcgccgtg gcgacgatca gcgccgcagt agccgcatag
    30901 atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg gtaaaaaatc aagaaactgc
    30961 ttcattttac gattccgtaa tcaaatgctt aaggttattg gcgaatcaac atatacaggc
    31021 ggaacagata gatgagcagt atggctgaaa tcaggttgct caaggtgttc gcaagtacgg
    31081 caccaatttc cggggttaat gcggcaaaag aagaggcaaa aagcagcaac agtgtttttg
    31141 ccagcaacca gctcagtact gcgggtgcca ccagacgcat attcgcccaa gtcagccgca
    31201 tactgctacg catcgaggca aaaacgccca ttttgtcctg taccaacatc accggtgcca
    31261 gggccagtaa aatggccatg ataattcccg gaacgaccac cagcatgatg ccaatctgta
    31321 ctaaaagggt agtcagaaaa atcagaataa ataactttgg caatatcggc gcactggcac
    31381 caatagctcg cagcgcactg actctctgac ccgcagacac cagctggata attaatatta
    31441 cgcctccggc gagaatggcg ttaccgatta atcctgaaaa agtggacgcc gctgaagcct
    31501 gcagcaaaat ttgttgctgt tccggtgaca tattctgaac caggtcgaac aacccactac
    31561 tgccgctaac gggcacgccg tcattgagct gcgcaagctg tgcatcactg ggtgagaaaa
    31621 catgccctaa caccactgtg ataaacgcac atagcaacga taccaacaga atggtcataa
    31681 attgattacg gaagaaattt ccggtgtcac ggtatacgga ctgcgccgtg atagacatgc
    31741 actctccttg agttttatgc aggtgttaat tagcgggcaa ttgtaccctg gttaagcctc
    31801 gggtggcagc atcaggtctt gtatggaaaa gcatatcttt gtaaagcgga ggtaattgct
    31861 ggccttcagg cgtgggcaaa ccgcggcaag tggcgaacga gttaatattt gcgtagcgaa
    31921 aatatttaaa aattgattta aatcacatta accaggattc tcaatgcaac ttctaaatta
    31981 atccagatca ataaatggtg aattatcata tgtaatgtga tctatgtagg atcatttgtt
    32041 actccaatgt aggtatattc gtcacgtttt tataaccata acgacggagc ggatatgaaa
    32101 aagttaacag tggcggcttt ggcagtaaca actcttctct ctggcagtgc ctatgcgcat
    32161 gaagcaggcg aattttttat gcgtgcaggg tctgcaaccg tacgtccaac agaaggtgct
    32221 ggtggtacgt taggaagtct gggtggattc agcgtgacca ataacacgca actgggcctg
    32281 acgtttactt atatggcgac cgataacatt ggtgtggaat tgctggcagc gacgccgttc
    32341 cgccataaaa tcggcacccg ggcgaccggc gatattgcaa ccgttcatca tctgccgcca
    32401 acactgatgg cgcagtggta ttttggtgat gccagcagca aattccgtcc ttacgttggt
    32461 gcaggtatta actacaccac cttctttgat aatggattta acgatcatgg caaagaggcg
    32521 gggctttccg atctcagtct taaagactcc tggggagctg ccgggcaggt gggggttgat
    32581 tatctgatta accgtgactg gttggttaac atgtcagtgt ggtacatgga tattgatacc
    32641 accgccaatt ataaactggg cggtgcacag caacacgata gcgtacgcct cgatccgtgg
    32701 gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac cccgtaatat
    32761 acggggtcaa taaggacatg gtataaaggg tattatttct tcgcttctac gccatcagtt
    32821 tcagagcgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc
    32881 atttcttttt cctcatttaa aatcgcttcg atggttggaa ttgaagctgt atcaccggca
    32941 ttttttgctg ctgctaatag tgaggtgtaa caggcgattt caaattgctc gaagacatat
    33001 ccgctaatag agcctttgac tatttcatca gaagggaata taccaccgat tgactgccca
    33061 agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc attacgatca
    33121 agaatagttt ccagttgaac aatctggttt ttggtttcac taagatgttg ttcaatacga
    33181 gcgcgtagtt caggataatt atctatacga ctagccatgg attcaagcat agattcggct
    33241 tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat aatgttcaat acgattcatg
    33301 aaaatgtcct ctttataaaa tgactaaaag ttaaagttca tatttcaggc tttattttcg
    33361 gctttcttat ttacgttatt aagtgccaga tcagtcagtt tgatgtcggt ggccttttct
    33421 tcttcaagag tttctttcag aagcttcgct gctttacggt aacctaattg ttcagccagc
    33481 gtcgctaatg tcccgtaact ggcgatctca tagtgctcga ctttctgcgc tgcggcaatc
    33541 agtgcggcat cacgcacttc gtttttctcg gtgctttcga taacctcatt agcttcttca
    33601 atgagacctt ccattgccac acatttcatg cgcttaattt tcaggttcga ttcagattcc
    33661 acaacttgat caatacgttc aatctgtcca tgagtttcct cgaggtgcgc atgaaaagcc
    33721 tgacttaatt tttcgtttga tgttgctctt gcgagttttg ccagtgcccg ggttaattgt
    33781 ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata catcttcaat ggtcttcata
    33841 ttcatatttt tctccagtga aatcactgcg agccatacgg cccgcagatg caattatctt
    33901 tatcagctga tattaataat taacggtgaa tcaggatttg cctgatttat taccaccgct
    33961 ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc
    34021 gccgctatgc tgaccgcctt tacggccagc gtcggatgcc ttttcacggt cttcggcgaa
    34081 atttcctgaa ccaccacgat gttcggccat gttatttctc ccgttgcgtt gcattgtttc
    34141 attaatatga gtgttgtgtg tcgacactca ttaaaattag tcgctaatga aaattagtca
    34201 aattaagcgc aacgagaaga aagagggaaa atatattatg aggaacattc tggatatatt
    34261 aacaattgcc tgaggaataa gtgacttaga gaaaattatt gactttactg gtgttatgtt
    34321 gcgggattaa tttgtttgat tatattactg ttgggctgaa aaaagacgta agtttacgtt
    34381 cgacaggggt aagcgaaacg gtaaaaagat aaataataaa tgaatttagg atttttccag
    34441 cttcgttaaa gacagttaaa atgccgccag cggaactggc ggctgtggga ttagctgcgc
    34501 gtcgccgctt tcatcggttg tacaaaagct ttcagtgccg ccagcatttt ctctggctca
    34561 ttaatatgtt gctcgatgat tttaacgatg gccgaaccag aaatcgcgcc cgcagctcct
    34621 gcatcaatcg ctgcttttac ctgatccggg gcggaaatac caaagccctg caatggaggc
    34681 gcagcattgt attctttcag cttcgtgacc agatgattga ggggtaacgc ggcgcggttt
    34741 tctgcgccgg tcacgcccgc acgtgacaat aaataggtgt atccacgacc gtaggaggct
    34801 atctggcgca gcaaatcatc gtcggcattc ggcgggcaaa taaagatagg tgcgacatta
    34861 tgacgcaacg cggcctggcg gaagggcgcg gactcttcca ctggcacatc ggcaaccagc
    34921 accgaatcga cgccgacttt ctcgcactgg gcataaaact catcaatgcc tttgttaaac
    34981 accaggttgg catacatcaa aaggccgatg ggaatggtcg ggtgcttctg gcgaatcagt
    35041 gccagcatct caaagcactg tgccggggtc acacctgccg caaaggcacg cagtgtggcg
    35101 ttttgaatcg tcgggccatc cgccagtggg tcggagaagg ggatacctaa ctccagcgcg
    35161 tcagcaccgg cttcaattag cgtgtcgata attttcagcg actgctcaat gcccggatca
    35221 ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct ccttcaactg ggcaaacaga
    35281 gattcgtagc gttccatcag atttcccctc gagctttcaa aatatcgtga acggtgaaga
    35341 tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag ctgctctttt tccgggtttt
    35401 cgcgcatcat tttcagcgca tgggccaggg cgtgggagga ctccagcgcc gggatgatcc
    35461 cttcgtgcag gcagagcgtt ttgaaggctt ccagtgcttc atcatcggta attgacacgt
    35521 aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg gccgacagac gggaaatcca
    35581 gtccggcgga gatggagtaa gattcttcaa tctgcccgtc ttcggtttgc atcatcggcg
    35641 ctttcatacc gaaatagatg cccacacgac catgttttaa cggtgcgccg tgctcgccag
    35701 tttcgatacc gtgaccgcca ggctccacac caatcaggcc gacgtcggtt tcgttgatga
    35761 aatcagcaaa catgccgatg gcattcgaac cgccgccaac acaggcgata accgcatccg
    35821 gcaggcgacc ttctctttcc agaatctgcg ctttcgtttc ttcgccaatc atccgctgaa
    35881 actcacgcac aatggtcggg taaggatgcg ggccagccgc ggtgcccagc atatagtgcg
    35941 cggtttcata actgccggac cagtcgcgta gcgcctcatt acaggcatct ttcagcgtcg
    36001 cggaaccgct atgcaccggg atcacttccg cacccattaa gcgcatacga aaaacgttag
    36061 gcgactggcg ctcgacgtct ttggcaccca tataaatgcg acatttcagg ccgagcaggg
    36121 cgctggcaag ggccgacgcc acgccatgct gaccggctcc ggtttcggcg atgatttccg
    36181 ttttacccat ccgcttcgcc agcaaagcct ggccgagcac ctggttggtt ttatgcgcgc
    36241 cgccgtgcag caaatcttcg cgcttgagat acagcgtggt gttagtgcca gccgtaatgt
    36301 tctggcattt ggtgagtgcg gttggacgcc cggcatagtt tttcagcagg tcgttgaact
    36361 gagcctgaaa ttcaggatct ttttgcgcgc tgacaaaagc ttcttccagc tggcgcagag
    36421 caggcatcag gatttgtggc acgtacatgc cgccaaactc accaaaatac gggttaagta
    36481 atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc tggaaaaccg aggccaaaag
    36541 acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa ttaaaatcaa gtccggcgca
    36601 gccggtttgt gccgcttcca cgcagttatc tgcgcctaag ccccccgcca gcagaacgtt
    36661 gccaagcgat tgaccgttta acagtgacca gtcgaaacgt tgaccgcttc caccctggcc
    36721 gttgtctaaa acatatttat caacgtgctg aagctcgcgg gcgggcaggg tttcaccgac
    36781 gcttaatgct ttccagatgg caacgtgtgc tggcagagct tcacgcagcg tatcgatata
    36841 cagctgatct tcattaccat gcagttgcac tgccgccagc gataacacct tagctttgtc
    36901 caccacatcg gcaatatcgt gattgcggaa cacgccaaca tactgcaacg gtgccgcagc
    36961 catcacttcc tgcgcctgtt caacgttgac gcaacgcggt gacgttgtaa caaaaatcaa
    37021 cccaccgtaa atcgcgcccg cgtcataagc ggctctagca tcttgcccac gcgtcaggcc
    37081 acatacttta ttctcaccca gcaacacccg gcgcacggcg gcgttcaaat cgtcatgggc
    37141 catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg cttaactcgc gcacctgagc
    37201 gtaagtattg atgccggatt cgctgattac cgtcacgttg tgccccagtt tcggcgcaag
    37261 ctcgcgggta cggttgagat caatcgacaa atcacgtaga tcgcggttgt tgatgccaac
    37321 gacctttgcc cccaatgcaa tggcgcgctc cagttcctct tcattactga cttcggtaag
    37381 cacgcccatc tccagactgt gggcgacggc ggcaaggtgg cgatattgtt cgtcatccag
    37441 tactgaaagc attaataagc aggcatcggc ctggtaatag cgcgccagat agatctggta
    37501 agggtcgata atgaagtctt tacataaaat cggctgcggg gcgatttggc tgacgattgg
    37561 gaggaaatca aagctcccct gaaaatattt ctcatcagtc agcaccgaaa ttgccgaagc
    37621 gtaatgttta taaatggcgg caatgcgtgc cggatcgaaa tcatcacgga tcacgccttt
    37681 tgacggcgag gctttcttgc actcaagaat aaacgccgtg cgtgcaccct gtagcgcatc
    37741 ataaaaatgt cgcgtgctcg gctgaatctc attctgaaaa ctggccagcg gttgctgctg
    37801 tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt ttcgctaaaa cggtttgcat
    37861 catttaccct cgtgccgcca gtgcggtgac tctgtcgtaa gcggaaccac tgcgcagtac
    37921 ctcaagaaca gtttgcgcat tggcttgcag atcttcatgg ccatgcaggc gcattaacat
    37981 ggcgacattc gccgcgacgg ctgcttcatg ggcggcgtcg cctttacctt gtaacaagcg
    38041 tgttaaaatg tcacggtttt cttccggtgt tccacctgcc agttgctcct ggtggtaggg
    38101 ggtcaggcca aaatcttcag cggtcaattg atagctctta atttcgccgt catgcagttc
    38161 ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc ccgccgctgt gcaccactgc
    38221 cgcgcgttga taccccagca cgcgcaaggt ttcggcaatc ggtagcacca gttccggact
    38281 ataaacacca attaacgcca gcggaggatg cgccgggtta atcagtggcc ccagcacgtt
    38341 gaacagggtg cgggttttca gttgctggcg aaccggcatc gcgtggcgaa atccggtgtg
    38401 atatttcggc gcaaagagga aacagacgcc taactcatcc agcgcctggc gcgatttatc
    38461 ggcgttcata tcaagattaa taccgaatgc cgccagcaga tccgacgaac cggatttact
    38521 ggagacacta cggttgccgt gtttcgccac tttcagccca caggccgcgg cgacaaacgc
    38581 actggcggta gaaatattga tactgttgct gccgtcacca ccggtgccga cgatatcggc
    38641 aaacagataa tccgggcgcg ggaatggcgc agcattttcc agaagggcag ttgctgcccc
    38701 ggcgatttcg ttcgggtgct cgccgcgaat tttcatgctc accagcgccg ccgcgagctg
    38761 ttctggcttc agctcgccac gcaccaccgc tgaaaacagc tggtggcttt cttgctggct
    38821 aagcgtctgc gcctgataca gtttttccag aatcggttgc agcgtgttag tcggttccag
    38881 tttctgctgc gcccaggcca atgtttgttc cagcaggcga gcgccctggg tagtaagaat
    38941 ggattccgga tggaactgga atccacaaac gcgatctgca tcgtgacgca ccgccatcac
    39001 catgccatta aaatgggcgt tgatggttaa accggccgga atgttactgc caaccagcga
    39061 gtgataacgc gccactggca gcgggtttgt taatccggca aacatcgcct gaccgtcatg
    39121 ttcaatgctg gaggctttac cgtgaagaat ttcgcccgct tgaccgacat accccccata
    39181 agcttcgaca atcgcctgat gaccgaggca aatgccaata attggcagct tgccgcgcag
    39241 gcgggtgagg agttccggca tacaaccggc ttcacttggc acaccggggc caggagaaag
    39301 catcagcacc ggattgctca ttgtcgccag gcgttcaatt aaggtctgcg ccggaatatg
    39361 gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac tgatctgcca ggttgtaagt
    39421 aaaagagtcg atattatcga gcagcagaat gtcagccatc agaacgtctc ctgtgcatga
    39481 tgcgcggtgg caatagcgcg cagtacagcg cgggctttat tacgggtttc gtcggcttcc
    39541 gactgcggaa cagaatcaag taccacgcca gcaccggctt gcacggtggc gataccgttt
    39601 tccaccagcg ccgagcggat cacaatgcag gtgtcgagat cgccatgcgc ggtgaaataa
    39661 cctaccgcgc cgccgtagct gccgcggcga cgaccttccg cctcggcaat taactgcata
    39721 gcgcgtactt tcggcgcacc gcttaacgtc cccatattca tacaggcgcg gtaagcgtgc
    39781 agggcgtcga gatcgtggcg cagctcaccg accacgcggg agaccaggtg catcacgtaa
    39841 gagtaacggt caactttggt gagatcggcg acgtagcggc tgccgggggt gcaaatgcgt
    39901 gccagatcat tacgggcgag atccaccagc atcagatgtt cagaaagctc tttatgatcg
    39961 gtacgcattt ccagttcgat gcggctgtcg aggtctctgt ccagcgagcc atcggcgcga
    40021 cgaccgcgcg ggcgtgttcc ggcaatcggg tagatctcaa tctggcggct ggtggcgtca
    40081 tacttgagcg aactttccgg cgacgcgcca aacagggtga aatcattatc ctgcataaaa
    40141 aacatgtacg ggctgggatt actctttttc agcacgtaat aggccgccag cggtgacggg
    40201 cagggcagag agaaacggcg agatggcacc acctggaaaa tttctccagc gcgaatcgct
    40261 ttttgcaaca aacgcactac gccaccgaac tcttcatcgc tctgattaca ttcacaacgc
    40321 atatgcggca cggaaaccac cggcagcggt ggcgcggctt cggtcagctg ctggcgaaga
    40381 tcgttcaggc gagcagtgag acgttgtttt tcttcttcat tcggagcaaa caggctggcc
    40441 tgaatgcggg tgcttttttt ctgatggtca atcaccatca gcgtttctgc gagataaaag
    40501 cagaaatcag gacagttatt ttccgctgcc agttgcggta aatcttcaaa tcccgccaca
    40561 aggtcataag agaacaggcc gccgaagaac atggcttctc gttcttcctt cggtacattc
    40621 aacagattct gcaataaacg gaaagcgtca aaaacagaaa gggagcataa gcgggcgtct
    40681 tcatccagca gtggactgac aggggggaag cgcagcacac ggcagtttgg tgattgttca
    40741 ctttccacac ccgcaggcag ggcgttatcc agtagtgcca gcagggcttc gccattgccg
    40801 gaaagtgcct gaattgtgac agtgtcacct aatgctgtaa tgcgcagcgc actgtctatc
    40861 agcagcaggc tttttaaatc atctttgctg tcgatatctg cggattccag cagcagcgtt
    40921 gctggacgat tcccacacaa ctggtgaaaa agcgcagtcg ggttgtcgcg ataagtgcct
    40981 tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca ttgttattct ctaattttgt
    41041 tcaaaaaaaa gcccgctcat taggcgggct gggtatctga ttgctttacg catggtgaat
    41101 acactgcccg tttcaggaag tgcgccacca acctttcagt acgaaaattg ctttcattgt
    41161 cgataccctt tttacgtgaa cttgcgtact agttaactag ttcgatgatt aattgtcaac
    41221 agctcatttc agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag
    41281 aacgggagtg cgccttgagc gacacgaatt acgcagtgat ttacgacctg cacagccata
    41341 ccacagcttc cgatggctgc ctgacgccag aagcattggt gcaccgtgca gtcgagatgc
    41401 gcgtcggcac cctggcgatc accgaccatg acaccacagc tgcaatctca ccagcaagag
    41461 aggaaatttc acgttcggga ctggcgctga atcttattcc cggcgtggaa atttccacgg
    41521 tctgggaaaa tcatgaaatt catattgtcg ggctgaatat tgatattact catccgctaa
    41581 tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca gcgggcgcag ctgattgctg
    41641 aaagacttga aaaagcgcaa atacctgggg cgctggaagg cgcgcaacga ctggcgcagg
    41701 gcggtgcggt gacgcgcggt cattttgcgc gtttccttgt ggagtgcggc aaagccagtt
    41761 caatggcgga tgtctttaaa aaatatctgg cgcgcgggaa aaccggatac gttccgccac
    41821 agtggtgtac aatagaacaa gctattgatg tcattcatca ttctggcggt aaggcggtgc
    41881 tagctcatcc tgggcggtac aatcttagtg ctaaatggct gaaaagattg gttgcgcatt
    41941 ttgccgaaca ccacggcgac gcgatggaag tcgcgcagtg ccagcaatcg cccaatgaac
    42001 gcacccagct ggccacactt gcgcgtcagc atcatttatg ggcatcacaa ggatctgatt
    42061 ttcatcagcc gtgcccgtgg attgaactgg gtcgtaaact ctggctgcct gcaggcgttg
    42121 aaggcgtctg gcagctatgg gaacagccgc agaacaccac agagagggaa ttatgagcca
    42181 gtttttttat attcatcctg ataacccgca gcaacgtctg atcaaccagg cggtggagat
    42241 cgtgcgtaaa ggcggggtga ttgtttatcc aactgattcc ggctatgcgc tcggctgtaa
    42301 aattgaagat aaaaacgcca tggagcgtat ttgtcgtatt cgccagctgc cggacggtca
    42361 caactttacc ctgatgtgtc gcgatctttc tgagctctcg acctattcat tcgtggataa
    42421 cgttgcgttt cgtttaatga aaaacaacac gccgggcaac tacaccttca tcctgaaagg
    42481 gacgaaggag gtgccacgcc gtctgttgca ggaaaaacgc aaaaccatcg gtatgcgtgt
    42541 gccttctaac ccgattgcgc aggcgctgct tgaagcattg ggcgaaccga tgctttccac
    42601 atcactgatg ctgccaggta gcgaatttac cgaatccgat ccggaagaaa ttaaagaccg
    42661 tctggaaaaa caggtagatt tgattattca cggcggctat ctcgggcaga aaccaacaac
    42721 ggttattgat cttactgatg atacgccggt cgtggtgcgt gaaggcgtag gtgatgtgaa
    42781 gcctttctta taacaaattg cgttccacgg atggaagact atggcaggga agtttcgctg
    42841 cattttgctg ttgatagcag ggctgtttgt atcatctcta agttatgcag aaaacacgga
    42901 gatcccttct tatgaagaag ggatctcgct ctttgatgtt gaagccaccc tgcaactgga
    42961 tggggtgctc gacatcaaag agaacattca ttttcaggcg cgaaatcagc agattaagca
    43021 cggattttat cgtgatttac cacgactatg gatgcagcct gatggtgacg ctgcactgct
    43081 gaactatcat attgttggcg tcacccgtga tggtgttcct gaaccctggc atcttgactg
    43141 gcatatcggt ttaatgagta ttgtcgtggg cgataaacaa cgtttcttgc ctcaaggtga
    43201 ctatcattat caaattcatt atcaggttaa aaatgctttc ctgcgtgagg gagattctga
    43261 tctgttaatc tggaacgtga ccggtaacaa gtggccgttt gaaatttata agacccgatt
    43321 ttctctccag atctctaata ttgcgggtaa tccatttagc gaaattgatc tctttaccgg
    43381 agaagagggc gacacatatc gaaatggccg catccttgag gacggaagaa ttgaatcccg
    43441 cgatccgttt tatcgtgaag acttcacggt cctctatcgc tggcctcacg ctttacttag
    43501 caatgccccg gctctacaaa cgacgaatat tttcagccat cttcttttac cctccacgtc
    43561 atcgttgtta atttggtttc cgtgtctgct catggtttgt ggatggttat atctctggaa
    43621 gcgcaggccg caatttacgc cggtagatgt gattgaaacc gatgtcattc cgccagatta
    43681 cacgcccggc atgttacgtc tcgatgcgaa gctggtttac gacgataaag gtttttgtgc
    43741 cgatatcgta aatctgattg tgaaaggcaa aattcatctg gaagatcagt atgacaagaa
    43801 ccagcaaatc ctgattcgtg ttaatgaagg cgcgaccaga aataatgcgg tattactgcc
    43861 cgcagagcag ttattactgg aagcgttatt tcgtaaaggc gataaggtcg ttcttacggg
    43921 gagacgcaac agagtgttac gcagggcatt tttacggatg cagaaatttt atctgccgcg
    43981 taaaaagtct tcgttttacc gacctgatgc gtttttgcaa tggggcggaa tggcaatatt
    44041 ggcggtcatt ctctacggta acctgagtcc cgtaggctgg gcaggaatga gtctggtggg
    44101 cgatatgttt attatgatct gctggcttct tcctttttta ttttgttccc ttgagctttt
    44161 gtttgcccgc gatgatgaca agccttgcgt taatcgtgta atcatcactt tgtttttacc
    44221 actgatttgt tcaggtgtgg ccttctattc tctctatatc aatgtcggag atgtattctt
    44281 ttactggtat atgccagcgg gttattttag cgctgttgtc ctgaccggtt atctcactgg
    44341 catggggtat atttttctgc caaagtttac ccaaactggg cagcaacgtt atgcccacgg
    44401 tgaagctatc gttaactatc ttgcgcgtaa agaggcggca acacacagtg ggcggcggcg
    44461 gaaaggggaa acacggaaac tggattacgc gttgttaggt tgggcggtct cggcaaacct
    44521 tggtagagaa tgggcagaac gtatcacccc atcactcaca gcggctgtta gcgccccgga
    44581 aattgcccgt agtggcgttt tattctcatt gcagacgcac ctgagttgcg gggcctatac
    44641 cagtttgttg gggcgaagct attccggtgg tggtgctggc ggcggtgcgg gtggcggagg
    44701 cggtggtggt tggtaaccaa atcgacgtta atcgaaacaa gctttaacca taaagatagt
    44761 gtagtatgtt gcgcctcaaa gccaggccga taaacgtcga gtcgtttact taaggcctga
    44821 agagttcaaa cagactttac tatcagtgag tcagatgtca gttctgactc actgttgcac
    44881 aaaagcatat cagacgcctg ggaaggcgac accaaaagga agctctatga gcgaaaagct
    44941 acagaaagtg ctggcgcgtg ccggccacgg ttctcgccgt gaaatcgaat ctattattga
    45001 agccggtcgt gtgagtgttg atggcaaaat tgctaaactc ggcgatcgcg ttgaagttac
    45061 ccctggattg aaaatccgta tcgatggtca ccttatttcg gtacgtgagt ccgctgaaca
    45121 aatttgtcgc gttctggcct attacaaacc ggaaggtgag ttgtgtaccc gtaacgatcc
    45181 ggaagggcgt ccaacggtgt ttgaccgtct gccaaagctg cgcggtgcgc gctggattgc
    45241 cgtgggtcgt ctggacgtta atacctgcgg tctgctgctg tttaccaccg acggtgaact
    45301 ggcaaaccgc ttaatgcatc caagccgtga agttgaacgt gaatatgccg tgcgtgtatt
    45361 tggtcaggtt gacgacgcga aactgcgtga tttgagtcgt ggcgtgcagt tggaagatgg
    45421 tccggcagcc tttaaaacca tcaagttcag cggcggcgaa gggatcaacc agtggtacaa
    45481 cgttactctg accgaaggcc gtaaccgtga agttcgtcgt ctttgggaag cggttggtgt
    45541 gcaggtaagc cgcctgatcc gtgttcgtta cggtgatatc ccactgccga aaggtctgcc
    45601 acgcggtggc tggacggagc tggacctcgc ccagactaac tatctgcgcg aactggtgga
    45661 gctaccgcca gaaaccagct ctaaagtcgc tgtagaaaaa gaccgtcgtc gtatgaaggc
    45721 gaatcagatt cgtcgagcgg ttaagcgtca cagtcaggtg agcggcggtc gtcgttctgg
    45781 tggacgtaat aataacggtt aatttaagat aagtataaaa taaaggccct gcggggcctt
    45841 ttcttcattt gaagcatgat aaatagtcgc agtattttaa tttaatttca tacttgctta
    45901 tgaataatac ccggaaatag ccacattact atttaattat ctgagataaa ctcaacgtca
    45961 agaggtgggt tatgattaga tatagtggaa tatttctatc gctgttaatg ctttcaggtt
    46021 gtgcaactga agcattgaaa aacgaacaag ctgaaactgt tattactgcg ggtaacaaag
    46081 cagtagcggc gaatgcaagt ttctttaaac aatactatgc ttctcgtgaa aacttccttg
    46141 ttaatttcta cgcgactaat ccggattgtg ctctgcgacc tgattatacc attgatatag
    46201 gcggtgatgg gaatggcgga aaattatgcc agcgttcagg gccaaataca aaacaagtac
    46261 aattagtccc tttcgagaaa gaaa
//
LOCUS       DARNVM010000015        45345 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.015, whole genome shotgun sequence.
ACCESSION   DARNVM010000015 DARNVM010000000
VERSION     DARNVM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.015"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(3..284)
                     /locus_tag="SI621_000851"
     CDS             complement(3..284)
                     /locus_tag="SI621_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HEI4063598.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQYCGQLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(547..1656)
                     /gene="apbC"
                     /locus_tag="SI621_000852"
     CDS             complement(547..1656)
                     /gene="apbC"
                     /locus_tag="SI621_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEI4063599.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1788..3821
                     /gene="metG"
                     /locus_tag="SI621_000853"
     CDS             1788..3821
                     /gene="metG"
                     /locus_tag="SI621_000853"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEI4063600.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGNTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWHGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3962..7759
                     /locus_tag="SI621_000854"
     CDS             3962..7759
                     /locus_tag="SI621_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HEI4063601.1"
                     /translation="MRHFIFQDEKSHKFWAVEQQGNELHICWGKVGTKGQNQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENALANVHIPPITKATPEVETSPESKNKRPWVADDAV
                     IPVTGDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQLAF
                     AELQRRILNNQQTGSIQSDAALLWNLWNSYSTDELVDDLVIRCGLETTVEIALLALQL
                     KYKPVKADVTTIIPPDLKAESLPNWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDAQWRKALVKLITATPDL
                     VTDVIPWVNAKAAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIPVFD
                     LPVLPIPAVTDITPGITELISHTDISRFSEIAQYQSSQQTLFTDLPLIEKESWETSFI
                     PFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHNNAN
                     KSWNLSALCYLPGQQAISFLNQIIIEERYSGEKEILAVFGSTAIPAFMTCLQRDHQRL
                     WIFTLFIGASELALPMAQRLQKKMAYKDAINWLANNPRHAAAGLLPLALGKPCQNREY
                     ARQAFRLLVKLNQRETIEEIGRRYNQPDVLAALATLFDSDPLEEYPAKIAPLPGFYQF
                     TLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITSYAGLATIKETFTHESLADFGWDL
                     YTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVYGLDVLADI
                     GSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDNSGS
                     LILDFGPRQFTVGFDETLKPVVHDANGKVLKDLPKPNQSDDQTLATDSVNLFKQLKKD
                     VHAIASQQIDRLEQDMCQRRRWTAEQFRLFLVEHPLVRHLTRRVLWGVYTEENTLIAC
                     FRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESATAFGQIYADYEKLPPFRQLDR
                     GYYHLTDNERDTHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGALE
                     LETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELINDIES
                     LFD"
     gene            7772..11566
                     /locus_tag="SI621_000855"
     CDS             7772..11566
                     /locus_tag="SI621_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HEI4063602.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEHAENATSKHTRTVTKSLELSDFSTIIQEQPSVTPEF
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQEIDLINSPKTTKLPSPVMAVLAALELHCTRYKER
                     ADVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPISYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLISEFKVFHSPTGHYWHLGILTTL
                     PLEKAVRAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARTWLLKYPEHAITGLLSAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNAMLALDPLDNHPTKIPTLPAFYQPSIWTRPVLKAN
                     AQSLPDSALQHLGEMLRFHQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAISSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPALGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYST
                     ADDDLFTLQEGDISVGIPHVLEISPTDAVAFGQLFADYELLPPFRQLERNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPADLSAEQVLSKLWLWEGKAESYGWGSNSTQEAQFSVLDDITASELINDIEAL
                     FE"
     gene            11576..15208
                     /locus_tag="SI621_000856"
     CDS             11576..15208
                     /locus_tag="SI621_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEI4063603.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRSWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPWTHFHSKEWLKVVAIDPRAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPTRPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMPWLSTQARSVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTVPRLDLAPF
                     ELTPQVYWQPGERERLAATESARYFSTESLAERMEHKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRREKSIWNLYLLAQLPREIALTFWLRINEKKHLFAGEDYFLSIFGLDALPGLL
                     LAFSYRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPT
                     RIPKAPDFWQPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTQMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEQLADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYENAF
                     VYDLGEYRLVLKFSSGFNHYNVDSKALMSFRSLHVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15269..15589
                     /locus_tag="SI621_000857"
     CDS             15269..15589
                     /locus_tag="SI621_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063604.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIEK"
     gene            16165..17242
                     /locus_tag="SI621_000858"
                     /pseudo
     CDS             16165..17242
                     /locus_tag="SI621_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            17253..19532
                     /locus_tag="SI621_000859"
                     /pseudo
     CDS             17253..19532
                     /locus_tag="SI621_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19525..20661
                     /locus_tag="SI621_000860"
     CDS             19525..20661
                     /locus_tag="SI621_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEI4063605.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGCSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20658..22658
                     /locus_tag="SI621_000861"
     CDS             20658..22658
                     /locus_tag="SI621_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEI4063606.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQTTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPSEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     DACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNNRLNCAQEMARRDSVGEVPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     VFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22783..23244
                     /locus_tag="SI621_000862"
     CDS             22783..23244
                     /locus_tag="SI621_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HEI4063607.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23286..23756)
                     /locus_tag="SI621_000863"
     CDS             complement(23286..23756)
                     /locus_tag="SI621_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEI4063608.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23803..24522)
                     /gene="yehT"
                     /locus_tag="SI621_000864"
     CDS             complement(23803..24522)
                     /gene="yehT"
                     /locus_tag="SI621_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HEI4063609.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRLERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24519..26204)
                     /gene="btsS"
                     /locus_tag="SI621_000865"
     CDS             complement(24519..26204)
                     /gene="btsS"
                     /locus_tag="SI621_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HEI4063610.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26426..27157
                     /gene="mlrA"
                     /locus_tag="SI621_000866"
     CDS             26426..27157
                     /gene="mlrA"
                     /locus_tag="SI621_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HEI4063611.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27217..27324
                     /locus_tag="SI621_000867"
     CDS             27217..27324
                     /locus_tag="SI621_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HEI4063612.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27305..28036)
                     /gene="yehW"
                     /locus_tag="SI621_000868"
     CDS             complement(27305..28036)
                     /gene="yehW"
                     /locus_tag="SI621_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HEI4063613.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(28041..28967)
                     /gene="yehX"
                     /locus_tag="SI621_000869"
     CDS             complement(28041..28967)
                     /gene="yehX"
                     /locus_tag="SI621_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HEI4063614.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(28960..30117)
                     /gene="yehY"
                     /locus_tag="SI621_000870"
     CDS             complement(28960..30117)
                     /gene="yehY"
                     /locus_tag="SI621_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HEI4063615.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(30124..31041)
                     /gene="osmF"
                     /locus_tag="SI621_000871"
     CDS             complement(30124..31041)
                     /gene="osmF"
                     /locus_tag="SI621_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HEI4063616.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31190..33487)
                     /gene="bglX"
                     /locus_tag="SI621_000872"
     CDS             complement(31190..33487)
                     /gene="bglX"
                     /locus_tag="SI621_000872"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEI4063617.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLEAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDATRHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33683..35398
                     /gene="dld"
                     /locus_tag="SI621_000873"
     CDS             33683..35398
                     /gene="dld"
                     /locus_tag="SI621_000873"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEI4063618.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35437..36369)
                     /gene="pbpG"
                     /locus_tag="SI621_000874"
     CDS             complement(35437..36369)
                     /gene="pbpG"
                     /locus_tag="SI621_000874"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEI4063619.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36543..37130)
                     /locus_tag="SI621_000875"
     CDS             complement(36543..37130)
                     /locus_tag="SI621_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEI4063620.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     DGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37300..37878
                     /locus_tag="SI621_000876"
     CDS             37300..37878
                     /locus_tag="SI621_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4063621.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(37997..38758)
                     /locus_tag="SI621_000877"
     CDS             complement(37997..38758)
                     /locus_tag="SI621_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4063622.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGVQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38811..40247)
                     /gene="mdtQ"
                     /locus_tag="SI621_000878"
     CDS             complement(38811..40247)
                     /gene="mdtQ"
                     /locus_tag="SI621_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HEI4063623.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40471..40554
                     /gene="yohP"
                     /locus_tag="SI621_000879"
     CDS             40471..40554
                     /gene="yohP"
                     /locus_tag="SI621_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HEI4063624.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(40927..41874)
                     /gene="dusC"
                     /locus_tag="SI621_000880"
     CDS             complement(40927..41874)
                     /gene="dusC"
                     /locus_tag="SI621_000880"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HEI4063625.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            42113..42511
                     /locus_tag="SI621_000881"
     CDS             42113..42511
                     /locus_tag="SI621_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEI4063626.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42508..43203
                     /locus_tag="SI621_000882"
     CDS             42508..43203
                     /locus_tag="SI621_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HEI4063627.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43333..44217
                     /gene="cdd"
                     /locus_tag="SI621_000883"
     CDS             43333..44217
                     /gene="cdd"
                     /locus_tag="SI621_000883"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEI4063628.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44367..45086
                     /gene="sanA"
                     /locus_tag="SI621_000884"
     CDS             44367..45086
                     /gene="sanA"
                     /locus_tag="SI621_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HEI4063629.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45089..45328
                     /locus_tag="SI621_000885"
     CDS             45089..45328
                     /locus_tag="SI621_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HEI4063630.1"
                     /translation="MDVQHFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT        11199 a        12010 c        11314 g        10822 t
ORIGIN      
        1 aattaatcgt agcgatttaa cacgattcga cgtttgctat cgctacctgc aacaaccact
       61 tctgtcgtta cacctttgac tggcgaagaa acctgatgac gggtatcaac atcatacgtt
      121 aactgaccac aatactgttg aggttgacca ttaaccatag ccatactgca tgttggggga
      181 ctgatcctgc cattaatcgt taatgtggca gtctgtgaag agaacgcggg agaagccatt
      241 ccataggcaa gaaaaataat tcctgacagc cagtacttat tcattgaacg ttatccctgt
      301 agtaaaggtt atgcctggat agaatgagtg cataacaaac tatagctgta catccactac
      361 acagccacga aggatgataa tgaagcattg cctgtacgat caatcgactt tatagagttt
      421 cggacgaagg tccgcagaat atttgcagca ttaaataagt gttcattaat gcggcaaaaa
      481 caaatacacc gcatcaatgt aacatctctg cggtgtatga aaaacaacca aaaaggttaa
      541 tacgcgttag accgcgcgga aggaaatctc gccaggaatg acttctccct gccaataaag
      601 ctgggctgca acgcggtcag ccagctggcg gtagatagcg gtaaattcgc tctccggacg
      661 gctgataacc gttggcgtgc ctttatcgag atcttcacgt aaggagatgt gtaacggcat
      721 ctgccccagc aactgcgtgt gatatttctc ggccagtttc tctgcaccac cggtaccgaa
      781 aatcggttca tggtgaccac agttactgca aatatgcaca ctcatgttct cgacaatacc
      841 gagcaccgga acctcgactt tctcgaacat cacaatgcct tttttcgcat cgatcaacgc
      901 aatgtcctgc ggagtggtga ccactaccgc gccagttact ggtatgtttt gtgccagtgt
      961 caattgaatg tcaccggtgc ctggcggcat atcaagcacc agatagtcga gatccggcca
     1021 caaggtttcc tgcaacatct gcattaacgc cttgctcgcc atcggaccac gccacaccat
     1081 cgcattgtcg tcagtgacca gataaccaat tgaattggtt gccagtccat gagacataat
     1141 cggtgccata tgggtaccgt ccggcgaagt tggacgctga ttttctgcac ccagcatggt
     1201 gggtattgat gggccataga tatcggcgtc cagaatgccg actttcgccc cttccgccgc
     1261 cagtgccagc gccaggttta ccgccgtgga ggatttaccc acgccgcctt taccggagct
     1321 gaccgcaatg atgttcttca cgccgttaat tcccggctga ttcttcacgc gttttaaggt
     1381 ggcgatattg tgcgacagtt tccagtcgat agctttcgcc ccggtgatgc gcagcagttc
     1441 ggcgctacat tgctctttta actcttcgaa cgcgctatgc cagacgaatg gcataaccag
     1501 ttccacatgc aacgtgtcgt ccatccaggc gacatggtgc aacgctttca gcgtggtgag
     1561 gttatgcttc agggttgggt gctgaaaatt ggccagtgtc ccggcgacca ttgcgcgcaa
     1621 ggcttctggt gatttggcct gggattgttc gttcatcccg actccttttt tgtatagata
     1681 aaccatcagc tgatagttta cctgaagaat atagagaagt acttacttaa catttttcca
     1741 tttggtacta tctaacccct tttcactatt aagaagtaat gcctactatg actcaagtcg
     1801 cgaagaaaat tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc cacctcggcc
     1861 atatgctgga gcacatccag gctgatgtct gggtccgtta ccagcgaatg cgcggccacg
     1921 aggtcaactt catctgcgcc gacgatgccc acggcacacc gatcatgctg aaagctcagc
     1981 agctcggtat caccccggag cagatgattg gcgaaatgag tcaggagcat cagactgatt
     2041 tcgcaggctt taacatcagc tatgacaact atcactcgac gcacagcgaa gagaaccgcc
     2101 agttgtcaga acttatctac tctcgcctga aagaaaacgg ttttattaaa aaccgcacca
     2161 tctctcagct ttacgatccg gaaaaaggca tgttcctgcc ggaccgtttt gtaaaaggca
     2221 cctgcccgaa atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc tgcggtgcga
     2281 cctacagccc gactgaactg atcgagccga aatcggtggt ttctggcgct accccggtaa
     2341 tgcgtgattc tgaacacttc ttctttgatc tgccctcttt cagcgaaatg ttgcaggcat
     2401 ggacccgcag cggtgcgttg caggagcagg tggcaaacaa aatgcaggag tggtttgaat
     2461 ccggcctgca acagtgggat atctcccgcg acgcccctta cttcggtttt gaaattccga
     2521 acgcgccggg caaatatttc tacgtctggc tggacgcacc gattggctac atgggttcct
     2581 tcaagaatct gtgcgacaaa cgcggcaaca ccaccagctt cgatgaatac tggaagaaag
     2641 attccaccgc cgagctgtac cacttcatcg gtaaagatat cgtttacttc cacagcctgt
     2701 tctggcctgc catgctggaa ggcagcaact tccgcaagcc gactaacctg tttgttcatg
     2761 gctatgtgac ggtgaacggc gcgaagatgt ccaagtctcg cggcaccttt attaaagcca
     2821 gtacctggct gaatcatttt gatgctgaca gcctgcgtta ctactacact gcgaaactct
     2881 cttcgcgcat tgatgatatc gatctcaacc tggaagattt cgttcagcgt gtgaatgccg
     2941 atatcgtgaa taaagtggtt aacctggcat cccgtaacgc tggctttatc aacaagcgtt
     3001 ttgacggcgt gctggcaagc gaactggctg acccgcagtt gtacaaaacc ttcacggatg
     3061 ccgctgaagt gattggtgaa gcgtgggaaa gccgcgaatt tggtaaagca gtacgcgaaa
     3121 ttatggcgct ggctgacctg gctaaccgct atgtcgatga acaggctccg tgggtggtgg
     3181 cgaaacagga aggccgcgat gccgacctgc aggcaatttg ctcgatgggc atcaacctgt
     3241 tccgcgtgct gatgacttac ctgaagccgg tactgccgaa actgaccgag cgtgcagaag
     3301 cattcctcaa tacagaactg acctggcatg gtatccagca accgctgttg ggccataaag
     3361 tgaatccgtt caaggcgctg tataaccgca tcgatatgaa gcaggttgaa gcactggtgg
     3421 aagcatctaa agaagaagta aaagccgctg ccgcgccggt aactggcccg ctggctgacg
     3481 atccaatcca ggaaactatc acctttgatg acttcgccaa agttgacctg cgcgtggcac
     3541 tgattgaaaa cgcagagttt gttgaaggtt ccgacaaact gctgcgcctg acgctggatc
     3601 tcggcggtga aaaacgcaat gtcttctccg gcattcgttc tgcttacccg gatccgcagg
     3661 cactgattgg tcgtcacacc attatggtgg ctaacctggc accacgtaaa atgcgcttcg
     3721 gtatctccga aggcatggtg atggctgccg gtcctggcgg gaaagatatt ttcctgctaa
     3781 gcccggatgc cggtgctaaa ccgggtcatc aggtgaaata atctcctttc aaggcgctgt
     3841 atcgacagcg ccttttcttt ataaattcct aaagttgttt tcttgcgatt ttgtctctct
     3901 ctaacccgca taaatactgg tagcatcagc attcaactgg ataaaattac agggatgcag
     3961 aatgaggcac tttatctttc aggacgaaaa atcacataaa ttctgggcgg tggagcaaca
     4021 gggaaacgag ttgcatatct gttggggaaa agttggcacc aaagggcaaa accaggtaaa
     4081 aagttttgcg gatgctgcgg cagcggcaaa agcggagctt aagctgatcg cagagaaaac
     4141 gaaaaaaggc tatgtggaaa atgctttagc aaacgtgcat atccccccca tcaccaaagc
     4201 cactcctgaa gttgaaacct cccctgagag taaaaacaaa cgcccatggg tggctgatga
     4261 tgcagtcatt ccggtaactg gcgatattaa tcgatttgct tttcctcatc gctctcgccc
     4321 cagagagatc aattatctgt acaaagatgg ccaaatatgg aagcgcatcg cgaataacac
     4381 cagggcatat gaccccgaca ataattaccg ctcttatcct gaaaactggc agctggcgtt
     4441 tgccgagtta caaaggcgaa ttctgaataa tcaacaaaca ggaagtatac aatctgatgc
     4501 ggcattgctc tggaatttat ggaactctta ctctacagat gaactggtcg atgacttagt
     4561 cattcgctgt ggtctggaaa ctaccgttga aatcgccctt ctcgctcttc aactcaaata
     4621 taaaccagta aaagccgacg tcaccaccat tattccccct gatctcaaag cagaatcact
     4681 accgaactgg catcaacgtc tatgtcatca tctttcactc gcttcagaag atgagtggca
     4741 acgctgtgta gataaagttc tcgctgctat tccctcgcta tccccagcac ggcaaccttt
     4801 tgcggcgtta ctgctccctg aacgcccgga tattgccaat gctatggcgc tacgttatgc
     4861 agatcaaaac gttccggcga tgacctggtt aagactggtg gtaaccgatg acgccgctct
     4921 gactacagca gaaaaatacg acttcccacc gttgtatcat gattttcgcg cttatctggc
     4981 aatgttgctg gcaaataatg gggtgcgcgg tgtaagccgg atacttcttg agtttacgga
     5041 agagcattct gacaatccca cttatctctt cgagcgcatc agcgagactg aagatcttgt
     5101 caaatggcta tggaaaacga atcacccgga tgcgattcaa attctgatcc tcggtgtaat
     5161 tggcaagaaa aagcacctgg aatacttaag caaagcctgt caaaaacatc ccgctgcggc
     5221 tcttgccgct tatgcaactt tactggcaat acatgaagat gcacagtggc gtaaagcgct
     5281 tgtcaaactg attaccgcca cgccagattt agtcactgac gtcattccct gggttaatgc
     5341 taaagcggca ggcatcttat ctgaatgtcg tactcagtta gtcgctgaag aatgtgaata
     5401 cgccactgcg gatatgttgc cggagctgtt tgtcgctcca ccgtgggtaa taaataagaa
     5461 aacgaatgta atacccgtat ttgatttacc ggtgcttccc atccctgcag tcactgacat
     5521 cacgccagga attacagagt taatcagtca tactgacata tccagattca gtgaaatcgc
     5581 gcaatatcag tcatcacagc aaaccttatt taccgacctg ccactaattg aaaaagaaag
     5641 ctgggagaca tctttcattc cttttacacc agaacaacaa atcctttggc aattaggttt
     5701 taatgaatgg ctgcattgcg aagatgactt acatgaaaaa aaatatatac cgcaaagcgc
     5761 tgttgatgcc ttgttgcgct ttgattttcc agcactaaaa gctgagtttg cgaaatatca
     5821 caacaatgcc aacaaaagct ggaatctatc tgcgctttgt tacttaccgg gacaacaggc
     5881 gatttcattt ctcaaccaaa taatcattga ggagcgatat tcaggtgaga aagagattct
     5941 ggcagttttt ggtagtacgg ccattcctgc gtttatgacc tgcttgcaac gagatcatca
     6001 acgactgtgg atattcaccc tctttattgg ggccagtgaa ctagccttac caatggcgca
     6061 gcggttacag aaaaaaatgg cttacaagga tgccattaat tggctggcaa ataacccgcg
     6121 ccatgccgcc gccgggctac tgcctttggc gctgggcaaa ccgtgccaaa atcgggaata
     6181 tgcccgccag gcattccgcc tgctagtgaa attaaaccag cgagaaacga ttgaagaaat
     6241 cggccggaga tacaaccaac ctgatgtttt agctgcgctg gcaacattgt tcgatagcga
     6301 tccgctggaa gaatatcccg ctaaaatcgc cccactgccc ggcttttatc agttcacctt
     6361 atggcgcaga ccacggctta aaagtaacaa cctgcctctg ccagatgacg ctatgcgcca
     6421 cctgggcaca atgttgagct ttccacgcga cattacctcc tacgctgggc tggcgaccat
     6481 caaagagacc ttcacccacg aatcactggc tgactttggc tgggatctct acacggcatg
     6541 gacagaagct ggcgcacccg caaaagaaaa ctgggcattt acttcgcttg ggattttagg
     6601 caatgacgac accgcacgta aattaacccc gcttatccgt gcctggccgg gtgaatccca
     6661 gcataaacgc gcggtttacg ggctggatgt attagctgat attggcagcg atgttgcgct
     6721 aatgctgctt aatggcatcg cgcgaaaaat taaattcaaa gcattacagg aacatgcccg
     6781 cgagaaaatc aacatagttg ctgaaaaccg tgggctgact atggctgaac tggaagaccg
     6841 cctggctcca gatttagggc ttgataacag tggctcgctg atactggatt tcggcccacg
     6901 tcaattcacc gttggttttg acgaaacctt aaaacctgta gtgcacgatg caaacggcaa
     6961 agtcctgaaa gatttaccca aaccaaatca gagcgatgat caaacactgg caactgactc
     7021 cgttaacctc ttcaaacaat tgaagaaaga tgtgcacgcc attgccagcc agcagattga
     7081 tcgtctggag caggatatgt gccagcgcag acgctggacg gcagagcagt tccgcctgtt
     7141 tctggtggag catccgctgg tacgtcactt aacccgacgg gtgttatggg gggtatatac
     7201 tgaggaaaac acgctgatcg cctgcttccg ggtcgcagaa gacagcactt acagcgatgc
     7261 gcaggatgag ttattcacgc tgccagcagg aaacatcggt ataccgcatg tgctggaaat
     7321 atctcctgaa tccgccactg catttgggca aatttatgct gattacgaaa agcttcctcc
     7381 ctttcgccaa ctcgatcgtg gttattacca ccttactgat aatgaacgtg atacccatga
     7441 attgatacgc tggcaaggac gtctttgcca ggccgggcgc attgtcgggc tggaacgtcg
     7501 aggctggcaa cgtctggaag aaagcggcag cgtctatgcg atgcgcaaat ctacccctta
     7561 tggcgctctc gaacttgaaa cagaacattt ttcattaatt tatggcgaaa cggggtatag
     7621 cgacctgcta cctgttgaaa gcgtaaaaat cacttcacca ggggaacgct acagtacaca
     7681 gccctcgctc acgttctccg cgcttgatgc catcaccgcc agcgaactca ttaacgacat
     7741 cgaatcactt tttgattaaa aggactttgc aatgaaaacc tatatttatc aggacgaaaa
     7801 atcacataaa ttctgggcgg tggagcagca gggaaacgaa ctgaatatca gttggggcaa
     7861 agtcggcacc aacgggcaaa gtcaggtaaa aagctttgca gatgctgcgg cggcggaaaa
     7921 agcggaactc aagctgattg cggagaaaac gaaaaagggg tatgtggaac atgcggagaa
     7981 tgctacatcg aaacatactc gaacggtaac gaaatctctc gaattatctg acttttctac
     8041 catcattcag gaacagccct cagttacacc agaattctac gtggctgaca aaaatacaga
     8101 cgttatttta ccgtggctgg caaaagatat tgctgtcgtt tttccgcttg aagtcgtacc
     8161 caccacctta agccatcgcc gctttcccgg agatcctgtt acacctgtag ataaattaac
     8221 ccagttacgt cgcttagcct gtggcgtgtc gcaacgggat aacaaaacag ctacatttga
     8281 cttcagcgcc tgtactttag agtggcaaaa cttcatcgct caggagatcg atctgattaa
     8341 cagcccgaaa acgacaaaat taccatcacc ggtaatggct gtactcgcgg cacttgaact
     8401 gcactgcacg agatataaag agcgtgcgga tgttatggat cagattattc aggaaggcgg
     8461 tctggaatat gctactgatg taataataca tcttcaacaa atatctattg aatgggatta
     8521 tgtcaacaat aatattgttt tcctgtcgtc tggcatttca cctgattatt tgcagcaata
     8581 ttccagcttt gaattacgcc tacgtaaaca tttatcactg gcggaagagt ctctctggca
     8641 aaaatgtgca caaaaactta ttgccgcagt tccacatatt ccagaatggc ggcaaccatt
     8701 aattgctttg ttattgcccg aaaaaccaga aattgcacat gaaattgccc agcgtctact
     8761 ggggcaaaag aaattaccct cgctggagtg gctaaaaata gtggcgactg atgagcacat
     8821 tcttgcctca ttagaaaaat atcacgagcc atatgccatt tttgatgatt actattgtgg
     8881 tgcgatatgg tcagccaccg tattacagga gcaaggtgtt gcggccctgc cccgatttgc
     8941 tccctatgtc gcaagtgact actgcgccga tgtgttgcgt catatcaatc atccgttcgc
     9001 attaacactg cttatacgtg tagcagggca tactaaacgc tgtcacgatc gaatgacgaa
     9061 agcctgcgct gcgttcccac acgcagcact ggcagcactg gcggaacttc tagtgcaaaa
     9121 agaagagaat agttggcgca ttatgctaat gacgatgctt atctcgcaac cgacactagc
     9181 agaacaggtc attccctggc tttcgacacc cgcagttgca gtgctgaaat catgccagca
     9241 acaactgaca cagccctcaa accatgccag cgccgatcta ctgccggcca tagtagtctc
     9301 cccaccatgg ctttcgaaaa agaaaaaatc gccgattcca gtgcttgatt tagcaccatt
     9361 aggtattgag ccaatcagtt atctgacaga agaaatcagt aatcaacttt tggcgaaata
     9421 tatctggtat tcaaaacaca tcacggttag tcatgaagaa agtactgcca atctgttggc
     9481 aaggatgggt tttcaacgac ggatcgctgg tacatatatt aaagctcccg aagcggtagt
     9541 tgaggcatgg ctaaatgaag attattcaac tttaataagt gaatttaagg tgtttcattc
     9601 acctaccggg cattattggc atttggggat tttgacaaca ttgccgctgg agaaagcagt
     9661 aagagcatgg aatgccctta ctctatctcc gcataccgat accgaatacg ccatgttaca
     9721 ttttggactc aaaggattac ctgggttagt aaactcactt gcacgctatc cacaggaagc
     9781 cttgcccata acgaattact tcgcagcgag tgagctggca cctgccgtcg cccgcgcctt
     9841 caacaaactc aaaaccctac gacaagacgc ccgcacctgg ctgctgaaat acccggaaca
     9901 cgccattacc ggcctgctgt ctgcggcgct cggcaaagcc ggtgaagcac aggataacgc
     9961 ccgcgctgca ttgcgtatgc ttaccgaaaa cggtcatcaa ccattactgc aagaaatcgc
    10021 ccggcgttat aaccagccgg aagtaacaga tgcggtgaac gctatgcttg cgctcgatcc
    10081 cttagataat cacccgacaa aaatccccac tcttccggcc ttttatcagc catcgatctg
    10141 gacccgcccg gtattaaaag caaatgccca atcactgccc gatagcgcac tccagcacct
    10201 cggtgaaatg ctccgcttcc atcaggagga ggcgctgtat ccgggattat tgcaggtgaa
    10261 agatgcctgt accgccgact cactggctga atttgcctgg gatctgttta ccgcctggca
    10321 gaccgctggc gcgccgtcga aagagagttg ggcgttcact gcgttaggcg ttctcggtaa
    10381 cgatgacacc gcccgcaaac tgacgccatt aatacgcgcc tggcctggtg aatcccagca
    10441 taaacgcgcc accgtcgggc tggatatcct cgccgctatc agtagtgata tcgccctgat
    10501 gcagcttaac ggcattgccc agaaactgaa attcaaagca ttacaggagc gggcgaaaga
    10561 gaaaattgcc gacattgccg aaagtcgcga actgacggta gcggagcttg aagatcggtt
    10621 agcaccggct ctcggtctgg atgataacgg ttcgctgctg ctggattttg gcccacggca
    10681 gttcaccgtc agctttgatg aaaccttaaa accgtttgtg cgcgatgttt ccggcagccg
    10741 cctgaaagac ttacctaaac ccaataaaag cgatgatgaa tcacaggcaa acgatgctgt
    10801 taaccgctac aaattgctga aaaaagatgc gcgtaccgtc gccgcccagc aggtagcaag
    10861 gctggaatcc gccatgtgcc tgcgccgccg ctggtcgccg gaaaactttc agctcttcct
    10921 ggttgagcat ccgctggttc gtcacttaac ccgccgtctg atttggggcg tttatagcgc
    10981 cgaaaaccag ctacaggcgt gctttagggt ggcggaagat aacagctaca gcaccgctga
    11041 cgatgatctt ttcaccttgc aggaaggcga tatctctgtt ggcattcctc acgttctgga
    11101 aatatcaccg acggatgctg tcgcctttgg tcagcttttt gccgactacg aactgctacc
    11161 accgttccgt cagcttgaac gtaacagtta cgccctgaca gaagccgagc gcaacgccag
    11221 cgaactgacc cgctgggcag gcagaaaatg cccgagcggc cgggtcatgg ggctggcaaa
    11281 taaaggctgg ataaagggcg aaccacagga tggaggctgg atcggatgga tgatcaaacc
    11341 tctcggtcgc tggtcgttaa tcatggaaat cgacgaaggt tttgcggtgg gtatgtcacc
    11401 agccgatctt agcgctgagc aggtcttaag caagctgtgg ctatgggaag gcaaagcgga
    11461 aagctatggc tgggggagta attcaactca ggaagcgcag ttctccgtgc tcgacgacat
    11521 caccgccagc gaactcatta acgatattga agccctgttt gaataaggaa agagcatgga
    11581 caaggaatta ccgtggctgg cggataacgc ccaagtggaa ctgaaatata aaaaaggcaa
    11641 aacgccgctc agccatcgca gttggccggg cgaaccggtg cctgttatca ctgaaagcct
    11701 catccagaca ctgggtgatg aattgctaca aaaagctgag aagaaaaaaa acattgtctg
    11761 gcgttatgaa aatttttcac tggagtggca gtcggccatt acgcaggcca tcaacttgat
    11821 tggcgaacac aaaccctcag ttccggcccg gacaatggcg gcgctagcct gtatcgcgca
    11881 aaatgatagc caacagttgc tcgacgaaat cgtccaacaa gagggactgg aatatgcgac
    11941 tgaggtggtg attgcacgcc agtttattac acgatgttat gaaagtgatc ctctgctagt
    12001 gacattgcag tatcagaacg aggattatgg ctatggttat cgatcagaaa cctataacga
    12061 attcgatctc cgactgcgta agcatctctc tctggcagag gaaagcagct ggcagcgttg
    12121 cgccgacaaa ctcattgccg cactgccagg aataaccaaa gttcgccgcc cttttattgc
    12181 tctgatcctc ccggaaaaac cagaaatcgc caatgagttg gtaagccttg aatgcccgtg
    12241 gactcatttt cattctaagg agtggttaaa agttgttgct attgacccca gagcggtgag
    12301 aaaactcgaa cgctactgga gccaggatat atttagcgat cgggaagcca gctacatgtc
    12361 gcatgaaaac cacttcggct acgcggcctg cgctgccctt ttgcgcgaac aaggactggc
    12421 agccgttcca cgcctggcaa tgtatgccca taaagaagat tgcggcagtc tgctggtaca
    12481 aattaaccat ccgcaggtca tccgcaccct gctgttagtt gctgataaaa acaaacccag
    12541 cctgcaacgt gtagctaaat acagtaaaaa tttcccccat gcgacgcttg ccgcactggc
    12601 agaactgctg gcgctaaaag aaccacctac gcgccctggt tatccaatca tcgaagacaa
    12661 aaagctgcct gcacagcaaa aagcacgtga tgaatactgg cgtacgctgt tacaaacgct
    12721 gatggcatcg cagccacaac tggcagaaga ggtgatgccg tggttaagta ctcaagcccg
    12781 gtcagtgctg aatagttatt tatcggcacc gcctaaaacg gttattgata gcaccgataa
    12841 cagccaaatg cctgaaatcc tcgtttcacc accgtggcgt agtaagaaaa aaatgacagt
    12901 tccacgtctt gatttggcac cgttcgaatt aacgccgcaa gtttactggc aaccaggcga
    12961 acgagagagg cttgccgcca ctgagtctgc ccgttatttc agcacggaat ctcttgcgga
    13021 acgcatggaa cataaaagtg gacgagttgt actacaggag ctgggttttg gagatgatgt
    13081 atggctgttt ctgaattata tactccccgg aaaactggat gctgcacgca attcactcat
    13141 tgttcagtgg cattactacc aggggcgggt tgaagagatc ctgaatggct ggaactcccc
    13201 ggaagcacaa ttagcagaac aggcgcttcg cagcggtcac gtagaagtgt taattaacat
    13261 atgggaaaat gacagctact cacgctatcg tcgggaaaag agtatctgga acctgtattt
    13321 attggcacag ttgccgcgtg agatagcttt gactttctgg ctacgtatca atgagaaaaa
    13381 gcatctgttc gcgggtgagg actattttct cagtatcttc ggattggatg ctctaccagg
    13441 tctgctgttg gctttttcat atcgtccaaa agaaacattt ccgttaattt taaattttgg
    13501 cgcaacagaa ctggccctgc ccgttgcccg cgtctggcga cgtttcgcgg cgcagcgtga
    13561 tctggctcgc cagtggattt tacaatggcc ggaacatacg gctactgcac ttatccctct
    13621 tgtcttcacc aaatccagcg ataatagcga agctgcatta cttgccctac gtttacttta
    13681 cgaacaggga catggtgaat tgctgcaaac cgcggcaaac cgctggcagc gtacagatgt
    13741 atggcctgcc ctggagcatt tacttaaaca gagtccaata gagatttacc cgacacgcat
    13801 tccaaaagcc cctgatttct ggcagccggc aatgtggtcc aggccgcgcc ttatcacgaa
    13861 taatcagcct gttaccgatg acgcactgga aattatcggc gaaatgctgc gctttacaca
    13921 gggcggacgt ttttatagcg gactggaaca actgaaaact ttctgccagc cgcaaacgct
    13981 ggcagctttt gcctgggatc ttttcaccgc gtggcaacaa gctggtgctc ccgcaaaaga
    14041 caactgggca tttctggcgt taagtctctt tggcgacgaa agcacggcac gggatctgac
    14101 gacacagatc cttgcctggc cacaggaagg caaatctgcc cgtgccgtca gcggcctgaa
    14161 catacttact cagatgaata atgatatggc actgatacag ctgcatcata tatcgcaacg
    14221 ggcgaaatcc cgccccttac gtgataacgc ggcggaattt cttcaggtgg tcgcagaaaa
    14281 tcgagggcta agccaggaac agttagcgga cagattagtc ccaacactgg gtcttgatga
    14341 tccgcaggcg ttgatttttg attttggtcc acggcaattt accgttcgct tcgatgaaaa
    14401 ccttaacccg gttatctttg atcagcaaaa cgttcgccag aaaagcgttc cccggttgcg
    14461 cgccgatgac gatcaactga aagcacccga ggcactggcc cgactaaaag ggctaaaaaa
    14521 agatgccact caggtgagca aaaacctgct cccgcgtctt gaaactgccc tgcgcaccac
    14581 ccgacgctgg tcgctggcag attttcattc tctgtttgtt aatcatccct ttacccgtct
    14641 ggttactcag cgattaatat ggggtgttta tccggcaaat gaaccgcgtc gtttactcaa
    14701 cgcctttcgt gtggccgctg agggggagtt ctgcaatgag caagatgagc caattgacct
    14761 gcctgccgat gctctgattg gcattgccca cccgttagaa atggcagcag aaatgcgcag
    14821 tgagtttgca cagctttttg ccgattacga aattatgccg ccttttcgcc aattggcacg
    14881 gcgcacagta ctgctcacgc ctgacgagtc agccagtaat agtttgaatc gctgggaagg
    14941 taaatctgct accgttgggc aacttatggg aatgcgatac aaaggctggg agtcaggcta
    15001 tgagaatgca tttgtctatg acctgggcga gtaccggctg gtccttaagt tttcatccgg
    15061 ttttaaccac tacaatgttg atagcaaagc gctaatgagc ttccgttctc ttcatgtata
    15121 ccgtgacaat aaatctgtca cttttgccga acttgatgtg tttgatttga gtgaagcgtt
    15181 aagcgcaccc gacgtcattt tccattaaaa cacagcgggc agtgatgcga ctgcccgtta
    15241 ttaacacgac ctgccaccgg gaaaagcgat gatcgtgcaa aaagagctgg ttgctattta
    15301 cgattatgag gtccctgtac ctgaagatcc gttttccttc agacttgaga tccataaatg
    15361 ctctgaatta tttacaggtt ccgtctaccg actggagcga ttccggctac atccaacatt
    15421 tcatcaacgt gatcgagagg atgtcgaccc gctaataaat gatgcgttga tttatataag
    15481 agatgagttt attgatgagc gaaaattacg gggcgaatca ccagaaacgg taatagccat
    15541 ttttaatcgc gaactacaga atatattcaa ccaagaaata gaaaaatagc tttattaaag
    15601 ggggaaacgg tattccccct tagatgcgtt tcaatttgcc cattacttca acaaatcaat
    15661 caaccgtaac agagccacta atagctccag tacggcaatg agcacggtta acttttgtgt
    15721 cagcgtgtcc atccgctctc cttattaagg agcgcaacac cagcacctta cccttacact
    15781 gcctgtcgat actggtttac gtatgttgga ccacgatgtt gcactccgga tcagggcacc
    15841 acctgcagca ccacccgtcc ggtagccagg acttaaaatt ggtccgatcc ggcgcaacgt
    15901 cccaaccgtc tggattataa atctcattat cttaattgca acggggtcca ccgatggtta
    15961 taattcgttc gttggaacac gatgttgcac aggctgcggt gttgggcctg aaaatagtaa
    16021 ataaaagaac ctgcctcacc agcaggtttt tttatttact gtgatctgct ttccagatat
    16081 ttttcgctca aacaactaat gcgccaaaca tttattgcgc gtaaaatatc gtttatttca
    16141 ttaatacatt tcagggatga atatatgtca ccgcagaata atcatctgca gcgtccgcct
    16201 gctgctgtgt tatacgccga tgaactggca aaattaaaac aaaatgataa cgcaccttgc
    16261 ccgcccggtt ggcagttaag tttgcctgcg gcccgtgctt ttatccttgg cgatagcgcg
    16321 caaaatatta gccgtaaagt ggtgattagt ccctccgctg tcgaacgtat gttagtcact
    16381 cttgccaccg ggcgcggttt gatgttggtg ggggaaccgg gcaccgcaaa atctctcctg
    16441 tctgaattac tggcaaccgc tattagcggc gatgccgggt taaccatcca gggcggcgca
    16501 tctactactg aagatcaaat ggaactacgc cctgcttatc aaccatggtc cgtctacgga
    16561 agcactggtc cctgccccgc tttaccaggg aatgcgcgac ggcaaaatcg tccgttttga
    16621 agagataacg cgtacaccgc tggaagtaca agactgtctg ttaggtatgc tttctgacag
    16681 ggtgatgacg gtgccggaac tcaccggtga agccagtcag ctctatgcgc gggaaggctt
    16741 taatattatt gccactgcca atacccgcga tcgcggcgtc aatgagatga gtgccgcgct
    16801 aaagcgtcgc ttcgattttg aaaccgtctt tccgattatg gatttcgccc aggagctgga
    16861 actggtcgcc agtgcttcgg cgcgtttgct ggcccatagc ggtattccgc ataaagtgcc
    16921 tgacgcggta ctggagttac tggtccgcac cttccgcgat ctgcgtgcca acggcgaaaa
    16981 gaaaacgtca atggatactc tgacggcaat tatatccacc gctgaagccg tgaacgtggc
    17041 ccacgcagtg ggcgtccgcg cctggttttt agcgaatcgc gcaggtgagc cagccgatct
    17101 ggtggactgt attgccggaa ccatcgttaa agataacgaa gaagatcgcg cgcgtctgcg
    17161 ccgttacttc gaacagcgtg tcgccacaca taaagaagct cactggcagg cttattatca
    17221 agcccgccac cgcctgccgt gaggaaagat gcatgagcga gccgttaatt gtcggcatcc
    17281 ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt aatcgaaagc cagcggccac
    17341 gatacgtgtt gattgaaggc ccggctgatt ttaatgaccg ggtagacgaa ctgtttttag
    17401 cccaccagct tccggtagct atttacagtt attgccagta tcaggacggt gcagcccccg
    17461 ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga gtggcaggcg ctacaagccg
    17521 cacgtcgtat tcaggcacaa acttacttca tcgatttgcc ttgctgggcg cagagtgaag
    17581 aagaagacga atcacccgat acgcaagatg acagccagac cttactgctg cgtgccaccc
    17641 gcatggataa cagcgacacc ctgtgggatc acttgttcga agatgaaagc cagcaaactg
    17701 cattaccctc tgcgctggcg cactattttg cacaactgcg gggcgattcc cccggagatg
    17761 ccctcaatcg tcagcgcgaa gcctttatgg cccgctggat tggatgggcg atgcagcaaa
    17821 acaatggcga cgtgttagtc gtctgcggcg gctggcatgc tccggcactg gcaaagatgt
    17881 ggcgcgaata cacgcaggaa attaacacgc cggaattgcc ctagctggca gatgccgtta
    17941 caggttgtta tctcaccccc tacagtgaaa agcgccttga tgtgctggca ggataccttt
    18001 ctggtatgcc tgccccggtc tggcaaaact ggtgctggca gtggggccta cagcaggccg
    18061 gtgaacaact gctaaaaact atccttaccc gtttgcgcca gcacaaattg cccgcttcta
    18121 ccgctgatat ggctgccgct catctgcatg cgatggcgct ggcacagttg cgcggtcata
    18181 cactaccgtt acgcactgac tggctggatg ccatagcagg ctcgctgatt aaagaagcct
    18241 tgaacgcgcc gttgccgtgg agctatcgcg gcgttattca tcccgatacc gatccgattc
    18301 tgctaacgtt gatagacaca ttagcgggtg acggattcgg taaacttgcc ccttctacgc
    18361 cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga acgtaccgga atctctcttc
    18421 cggcggagct taccttaaat cgctttaccc ccgatggact ggcgcaaagt caggtgttac
    18481 atcggttggc aatactggag atcccagggg ttgtgcgcca gcagggaagt acactgtcac
    18541 ttgcaggcaa cggtgaagaa tgctggaaat taacccgccc gcttagccag catgcggcat
    18601 tgattgaggc cgcctgcttt ggtgccacac tccaggaagc cgcacgccat aaattagaag
    18661 ccgatatgct ggacgcgggt ggaatcagca tcatcaccac atgtcttagc caggcggcgt
    18721 tagcgggtct ggcgtctttc agtcaacaat tactggagca acttacatta ttaatcgccc
    18781 aggaaaatca atttgccgaa atgggccagg cgctggaagt gctatatgcc ttatggcggt
    18841 tagatgaaat cagcggtatg caaggcgcgc agatattgca gacgacgtta tgcgcggcta
    18901 tcgatcgcac actctggctg tgtgaatcca acggcaaacc ggaggaaaag gagtttcacg
    18961 ctcacctgca tagctggcag gcgctttgcc atatcctgcg cgatctacat agcggcgtta
    19021 atttacccgg cgtttcgctt tctgcggcgg tagccttact ggagcgccgc agtcaggcga
    19081 ttcatgcccc ggcgctggat cgcggcgcgg ctcttggcgc actaatgcgt ctggaacatc
    19141 ccaacgccag tgccgaagcg gcgctgacaa tgctggcgca attatccccg gcacaatccg
    19201 gcgaagcgct gcacggtttg ctggcgctgg cccgccatca actggcctgt cagccggcat
    19261 ttatcgccgg tttcagtagt catttaaatc aactgagtaa cgacgatttt attaacgccc
    19321 tgcccgattt acgcgcggcg atggcctggc taccaccacg agaacgcggg acgctggcgc
    19381 atcaggtgtt tgagcattat caactggcgc aacttcccgt ttcggcactg caaatgccgt
    19441 tgcattgtcc accgcaagcc attgcacatc atcaacaact cgaacagcag gcactggcat
    19501 cgctgcaaca ctggggagtt ttccatgtct gaactgaacg atcttctgac cacccgtgag
    19561 ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa cgaccctttg tgggctggat
    19621 gacaatgccc ggcagataga ccacgcgctg gagtggctgt atgggcgcga tcctgaacgg
    19681 ctccagcgtg gtgaacgctc cggtggatta ggttgctcaa atctcaccac ccctgagtgg
    19741 atcaacagta ttcacacgct gtttccgcaa caggtgatcg agcggctgga aagcgatgcc
    19801 gtgctgcgct atggcattga agatgtggtg acgaatctcg acgtactgga acgtatgcag
    19861 ccttctgaaa gcctgctacg cgccgttttg cacaccaaac atctgatgaa tcccgaagtg
    19921 ctggctgccg ctcgccggat agtgcgccag gttgttgaag agattatggc gcgactggca
    19981 aaggaagttc gccaggcttt ttctggtgtc cgcgatcgcc gccgtcgctc atttattcca
    20041 ctggcgcgta atttcgattt caaaagtacg ctgcgtgcca acctgcaaca ctggcatccg
    20101 caacacggca agttgtatat cgaatccccc cgctttaaca gccgcattaa gcgccatagc
    20161 gatcaatggc aactggtctt actggttgat caaagcggat cgatggttga ctcggtgatc
    20221 cactcagcgg tgatggcggc ctgtttatgg cagttacccg gtattcgtac ccatctggtg
    20281 gcgtttgaca ccagcgtggt tgatctcacg gcagacgttg ccgatccggt agagttatta
    20341 atgaaagtgc aattaggcgg cggaaccaat atcgccagtg ccatggaata tggtcggcaa
    20401 cttattgaac aaccagcgaa aagcgtcatt atcctcgtga gcgattttta cgaagggggt
    20461 tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg
    20521 ggactggcgg cgctcgatag caccgcaaca ccttgctatg accacgatac ggcccaggcg
    20581 ctggttaatg tcggcgcaca aatagccgcc atgacaccgg gcgagctggc atcatggctt
    20641 gcggagaatc ttcagtcatg aattcactac gtccggaatt attagaactg acaccgcagg
    20701 ccctgacagc gttaagcaat gccggttttg ttaagcgcag tcttaaggaa ctggaaaatg
    20761 gcaacgtccc ggagatcagc catgagaacg gcgctttaat cgccaccttc agtgacggtg
    20821 tccgtaccca gctggcgaac ggtcaggcac tgaaagaggc tcagtgcagc tgcggggcca
    20881 gcggcatgtg ccgccatcgc gtgatgctgg ttttaagtta tcagcgactt tgcgccacca
    20941 cccagactac ggaaaaagaa gaagagtggg atccggcaat ctggctggaa gaactggcta
    21001 ccctgcccga tgccacccgc aaacgcgcac aggcactggt cgctaaaggc atcaccattg
    21061 agttgttctg tacgccgagc gaaattccct ctgcccgctt accgatgagc gatgtacgtt
    21121 tttattcccg cagcagcatt cgtttcgctc gctgtgattg tattgaagga acactttgcg
    21181 aacatgtcgt actggcagta caggcctttg tccaggccaa agcgcagcaa gcggaattta
    21241 ctcatttaat ctggcagatg cgcagcgaac acgtcacatc atctgacgat ccgtttgcca
    21301 acgatgaggg ggacgcgtgt cgtcaatatg ttcagcaatt aagccaggcg ttatggcttg
    21361 gcggcatcag ccagccgctt attcattacg aggctgcttt cagccgcgcg cagcaagcgg
    21421 cggaacgctg caactggcgc tgggtgagtg aatcgctacg gcaactgcgc gccagcgttg
    21481 atgccttcca cgcccgcgcc agccactatc atgccggaga atgcttacgt cagcttgcgg
    21541 cattaaacaa tcgattaaat tgtgcacaag agatggcccg gcgcgacagt gttggtgaag
    21601 ttcctcccat gccgtggcgc acggtcgttg gcgctggcat tgccggagaa gcaaagcttg
    21661 atcatctgcg gctggtgtct ttaggtatgc gttgctggca ggatattgag cagtatggtt
    21721 tacgcatctg gtttaccgat cccgacaccg gcagtatttt gcatctttcg cgtagttggc
    21781 cgagaagcga acaggaaaac tcaccggcag cgacgcgtcg gctgtttagt tttcaggctg
    21841 gcgcactggc tggtgggcaa attgtttcac aagcagcaaa acgcagtgcc gatggcgagc
    21901 tgctgttagc tacccgcaac cgcttgagta gtgttgttcc actgtcgcca gacgcctggc
    21961 aaatgttgag tgcgccgttg cgccagccgg gcattgtggc tttgcgtgaa tatttacgcc
    22021 agcgtccccc cgcctgcata cggcctctta atcaggtgga taacttattt attctgccag
    22081 tcgctgagtg tatttcactc ggttgggata gcagccgcca gacgctggac gcgcaggtaa
    22141 tcagcggcga aggtgaagat aatctgctga cgttatccct tccggcatct gcctgttctc
    22201 cttttgccgt tgaacgcatg gcggcgcttt tgcaacaaac agacgacccc gtgagtctgg
    22261 tttctggctt tgtcagcttt gtagaggggc aattgacact ggaaccacgg gtaatgatga
    22321 ccaaaacccg cgcctgggcg ctggacgcag aaactgcgcc tgtggcaccg ctaccttctg
    22381 ccagcgtttt gcctgtgccg tctaccgctc atcagttgct gatgcgctgc caggcgttac
    22441 ttattcaact gctccataac ggctggcgct atcaggaaca gagtgctatt ggtcaggcag
    22501 agttgctggc gaatgacctc accgcggtgg ttttttatcg gctggcacat gtgttggcac
    22561 aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttacttt
    22621 gcgaacaatt attccccatg cttcagcaac aaggatgaaa tagtgctttt tactaagagt
    22681 tctactccag ttccggactg ctcacgtcac ggtattaggc atatcctata tagctcctgg
    22741 tgagagtcac cagtttcttg attaaataaa atggagtttt acatgaaggc tttcaataag
    22801 ctgttttccc tcgttgttgc atctgttctg gttttctctc ttgctggctg cggtgacaaa
    22861 gaagaatcga aaaaattcag cgccaatctg aacggtactg aaattgccat tacctatgtc
    22921 tacaaaggtg acaaggtgct taagcaatct tctgaaacca aaattcaatt tgcctccatt
    22981 ggtgcaacca ccaaagaaga tgctgccaag acacttgagc cgttaagcgc caaatacaaa
    23041 aacatcgcgg gtgttgaaga aaaattaacc tataccgata cctacgcgca ggaaaacgtg
    23101 actatcgata tggaaaaagt ggatttcaaa gccctgcagg gtatttcagg aatcaacgtt
    23161 tctgctgaag atgccaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc
    23221 gctggtttta aagaagtgaa ataatctgtc ggcggccatg tttttgcatg gccgccatac
    23281 ccgtcttagc ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa
    23341 aattgatcgc cgcattcgcg gaagttttta tgatccggtg cacgcatcat cgccgtaatt
    23401 tccggcatcg aaacgcggaa ctgctgttcg gtgaggatcg ccagaatgtc atcggtcttc
    23461 agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc
    23521 tccagtgccg gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaag
    23581 gaagacaaaa caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag
    23641 acggcgatct gttccgcggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca
    23701 ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg
    23761 ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc
    23821 ttttaagctt ttcagataac gacggctgac cggcacggtt aagccattac gcaaaatcaa
    23881 ctcggcctgg ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata
    23941 ctgacgatgg cagcgtagta gtggtgtacg actctccagg gtacgtaatg tcaattcggt
    24001 aaagccctct ttcccttcgt gactggtaac gtagacaccg ctcatccgac tgctgacaaa
    24061 tgctacatct ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt
    24121 cagcgcctgt tgattttccg gtaacagcga aacatcctgc ttgctgcgct ccagacgcaa
    24181 tcgcgccagc gttttctcca gtcgcgcttc atcaattggc ttcagcagat aatcaaaagc
    24241 atgttcttca aaggctttaa tggcgtattc atcaaacgcg gtgagaaaaa cgatatacgg
    24301 acgatgttcc gggtcgagca tccccaccat ttccagacca ctgatgcgcg gcatctggat
    24361 atcaagaaac agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ccacggcgtt
    24421 tgaacactct ccaacgattt caatatcgct ctgctcctgc aaaaatacgc gcaggttctc
    24481 ccgtgctaac ggctcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt
    24541 agtcgtaacg ttattcgggt gtaactatca ggctcacagg cgacgcttat cccatagtca
    24601 tcgccaaacc gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg
    24661 gttaccggtt gatacaaacc ggcgttgtct tcgatctcca gcatcaaatg ttgcccctca
    24721 cgtcgggcgc tgattgccac tcgccctgta tccagcagtt gtgatgtccc atgtttaata
    24781 gcgttttcca ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc
    24841 ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa
    24901 gcgttcacat gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt
    24961 ttgcggaaaa aagtggaaag atactgcacc agctggctgg cctgttcgct gtcgcggcgg
    25021 atcaccgctt taatggtgtt aagcgcatta aacaaaaaat gggggttcac ctgggcgtga
    25081 agcagtttga tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg
    25141 atctgcgccg aaagcagttg cgcaatcccc tcacccagcg tgcgattgat tgaactgaat
    25201 aaacggtttt tggcttcata caatttgatg gtacccatca cccgctgatt ttcaccacgc
    25261 aacggaatta ccagcgtcga ccccagtttg cattgcggat gcaaagagca acgataaggt
    25321 acttcgttgc catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtgtaagtc
    25381 gaagaaatcg gtttgccggg taaatggtga tcgtcaccaa ttccggtaaa ggccagcaat
    25441 ttctctcgat cggtaatcgc gactgcacca atatccagct cctgatacag cacctgtgcc
    25501 actttcatgc tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggcagcc
    25561 acttttagcg cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg
    25621 agcaatatac gcataaacag cgccgcgccg acggtattgg tgaccatcat tggcgcagca
    25681 atattactca ccagacgcac cgcatcttca taaggtcggg cgattgccag gatgatcaac
    25741 atttgcacca tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta
    25801 tcagtgcgcc cgcgacggat caggatgctg tgtaccaggc cgccgagcaa cccttcaacg
    25861 atggtcgaga tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg
    25921 ccggtcagac caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgcg
    25981 cctatcgcac gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg
    26041 atgcagaaga tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc
    26101 tgcattaacg gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg
    26161 cacatctgct gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact
    26221 tccctttaaa acgcgtaaca tacattgcct gcgtttaact ttctttgaac tcttgcagaa
    26281 aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat
    26341 agatgcacaa aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg
    26401 ggacctcgcg agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct
    26461 ttgtgatatt aaccctgtca cgttacgcgc gtggcagagg cgttacggat tgctgaaacc
    26521 gcaacggaca gacggcggcc atcggctgtt caacgatgcc gatattgacc ggatccgcga
    26581 gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa gttaaaatgc tgctcagtaa
    26641 tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacttacct
    26701 gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc
    26761 cgcccagacg ctcaccacac atctgtttat tcctctgcgc cgacgacttc agtgccaaca
    26821 accgactctc caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatcgccat
    26881 ttgcctggct tcggcacgta aaaaacaggg taaagatgcg ttggtggttg gctggaatat
    26941 tcatgatacc acccgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat
    27001 tgatgtcctc gcccactcgc tcaatcaact acgccctgaa ctgttcgaag gccgtacatt
    27061 gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag cagcaactca ccagttggca
    27121 ggaacaaggc catgatattt tcccactcgg catttaatga ttcgttaaca aatgcgcttt
    27181 actgtacaat cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc
    27241 atcgccctgt tcctgtttat ggcgttaggc ggaattggtg gcgtcatgct cgcaggttat
    27301 acctttattt tgcgtgctgg ctaagcgcct gcaccagcct ttcaaacagg cggtctgcaa
    27361 tgatcgccgc cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc
    27421 taagcccgat gatgatcggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg
    27481 ccgtaccaat gttgataatc accgaagttc gcacgcccgc cagaatcacc ggagccgcca
    27541 gcggtagctc gaccttacgc agtcgctgac cacggctcat tcccatacct ttcgcaactt
    27601 ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt tgcctgcagg acgggcagca
    27661 caccataaag gatcaaggcg ataatcgcgg gttgcagacc aaacccaatc accggaacgg
    27721 caatcgccag caccgcgacg ggcggaaaag tctgtccaac ggcggcaata gtttccacca
    27781 gtgggcgaaa ttccgcgccc cacgggcgag taacaataat tcctgcacca gtgccaatga
    27841 tcaccgcaaa caaactcgaa attcccacca gccagaaatg agccagtgcc agagcagcaa
    27901 aactttcttg ctgataaacg ggtcgtggca gttgtgggaa caaggcagca aacagcggct
    27961 ggctgtaagg cagccagaaa atcagcgcca caaacagagc aatgagccag aacagcggat
    28021 cgcgcaacat cttcatacgt ttacgcctcc accagcagat cctgaaaatg cagcgtgccg
    28081 caaggctggc cctgcgtgtt caccaccggc agcacctcgc atccccgcgc gacaaacagg
    28141 gagagcgcat cgcgtagagt catctcttct gccagcgcct caccttctgc ccgttcttcg
    28201 cgacgcacgt aatccgccac gctacgtaac gaaagcaggc ggacacccag ttcactatgc
    28261 ccaaaaaact ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag cggattgccc
    28321 tgctgcacca cttcaccgtg atccatcaac accagatgtt ctgccagtcg tagcgcctca
    28381 tcaatatcat gagtgaccag cacaatggta cgccccagca aacggtgtat gcgcgtcatc
    28441 tcttgttgca acgcgccgcg cgttaccggg tccagcgcgc caaaaggttc atccatcagt
    28501 aagacttgcg gatcggcagc cagtgcgcgc gccactccca cacgttgctg ctgaccgcca
    28561 gaaagctgat gcggataacg ctcacgcaaa tttggctcca gccccagtag cgccattaat
    28621 tcgtcgatac gatcgtcaat ccgcgcccgc gaccattttt gtaattgcgg cacggtggca
    28681 atattttgcg ccacgctcca gtggggaaac aggccaatag attgaatggc atagcccatc
    28741 cggcggcgca actccagcac tggcagcgag cgaatttctt ctccggcaaa gcggatcacg
    28801 ccgctgtcat gctccaccag gcggttaatc attttcaggg tggtggattt accggagcct
    28861 gatgtaccaa tcagcaccga aaaactcccc tcctgaaaat tgagattgag atcgttaacg
    28921 gctttttgtg cgccgaacag tttgctgaca tggctaaatt caatcattac gtttcacctt
    28981 cagcagtgcg ataagcaaat cgaacagcgc atcgatcagc accgccagaa caattaccgg
    29041 gatcaccccc agcaacacta aatcaatggc actgcttagc agcccctgga aaaccagcgc
    29101 accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc atgccgacgg tttgcaccat
    29161 caccacccgc aggctacgca gaaataccgg taacgccagc ggtaactgaa catgcaggaa
    29221 tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg
    29281 gttcaagccg actaccacgc cgcgcaccag cggcagcaag gcatagagca ccagcgcaat
    29341 cagtgcgggt gtcattccgg ttcctgctat gccgagctta cccagccacg gaaaggccgt
    29401 aaccagtccg gcaagcggcg caatcaacaa gccaaagagc gccaccgaag gcacggtctg
    29461 aatgacattg agtagcgaaa aaatcgcccc ctgccgcgca gtggaaaagt agcaccagac
    29521 gcccaacggc acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg
    29581 ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg tattctttca ttagcgagag
    29641 atcgttaagc gtgccggagt acagcaacca cagcggaata atggcaatct gcatatgcaa
    29701 caaccagcgc cacagcggat gcgtggagat tcggcggatg gcatcgctac aggccagcaa
    29761 tgccagcgcc gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc
    29821 attttgtgcc agttgtgtcg ccgcctttcc tgctccccac accagcaata cgaagacgaa
    29881 ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg ggaataaaac aggctgtcat
    29941 ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc ggccacagct gccagagatg
    30001 acgcccctca cccgaaacta aacgattagg cgcgtaactg ataaatggca gcgccgctgc
    30061 gattgccgtc agcaacagca gcagcgccag aacaggatta atacggaaat aagtcacggg
    30121 aaattacttc gtccaccctt tttgtttcag gtagtcggca gccacttttt tggcatccag
    30181 tccttccaca gcaatgctgg cattcagttg ctgcaatgtt ttttcatcga ggctggcgaa
    30241 gactggctgt agccactgtg ccatttgtgg atactctttc aacaccgact cacgcaccac
    30301 tggtgcaggc gcgtagatag gttgcacgcc ttgcggatca cttaaggttt gcagccccag
    30361 cgccgcgacc gggccgtcag tgccgtaagc catcgccgca ttaacgccag aggtttgctg
    30421 ggcagcggct ttgatcgtca ccgccgtgtc gccaccagcc agtgacagca actgatcctg
    30481 accgagctta aagccatagg ctttttcaaa cgcgggtaac gcatcggcgc gttcaataaa
    30541 ttctgccgag gccgccagtt tgaaggtgcc gccctctttc agataacgac tcaggtcggc
    30601 aagcgaagtg agtttgtttt tctctgccac atcctgacgc acggcgatag tccaggtgtt
    30661 atttgcaggc gcgggcgtca gccagattaa cttgttttgc tctgcatcga gttttttgac
    30721 tttctcgtag ccttgcccgg catttttcca tgccgcatcg ttttcatctt taaagaaaaa
    30781 cgcgccattg ccggtatatt ccggatagat atccagttca ccggaagtaa tcgccccgcg
    30841 caccacggga gtcgttccca gttgcacttt atttaccgta ggaacaccgt gactttccag
    30901 cacctgcaaa atgatattgc cgagcagcgc gccttcggta tcgatttttg aaccgacctt
    30961 aacgggggaa gccgcctgta gcggcaggct cacggctgcc aacaaaacca atgaacctgc
    31021 ccagagcttt gagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat
    31081 taagagaaaa gcatagttga taatgacagg gttagcctgt ctgggcggat tcagttgcag
    31141 aatcagataa atacttaaag ggcgcagacg tcgcgccctt actaaagcat tacagcaact
    31201 caaactcgcc tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag
    31261 gctcggcgtc atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga
    31321 agctgacggt ctgggtttcg cccggtttca gggtgatttt ctcaaagcct ttcagctgct
    31381 tcaccggacg actcatggaa gccgtcacat cctgcaagta catctgcact accgtcgccc
    31441 cttcgcgctt accggtgttc gtcacctgca cactggcggt cactttgccg tcacgcttca
    31501 tggtcggcgc agaaagtttc acatcagaga cggtgaaagt ggtatagctc agaccatagc
    31561 cgaacggata aagcgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct
    31621 tatcggcgtt atacgggcga ccggtattca gatggctgta gtacacaggg atctgcccga
    31681 cagaacgcgg gaaggacatc ggcagcttgc cggacgggtt gtaatcgcca aacaatacat
    31741 cggcaatcgc attcccccct tcagtccccg caaaccaggt ttccagaatc gcatcagcct
    31801 gctgatcttc tttcaccagc gccagcggac gcccattcat cagcaccagc accagcggtt
    31861 taccggtggc tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg
    31921 tacggctgga agcctcgtgc gccatcccct gtgcttcacc gaccacagcc accaccacat
    31981 cagattgttt cgcggtttgc accgcttcat caatcatctc ttgcggcgaa cgcggatcga
    32041 ctttgaccgc ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt
    32101 tagccccttt ggcatacagc actttaccgt tttcaccgac ggcattttta atcccggtca
    32161 gcacggtcac ggattgatcg gcaacccctg ccgccgacca gctgcccatc acgtcacgct
    32221 tgctgtctgc cagcgggcca accaccgcaa tggttgcgga ttttttcagc ggtaacgttt
    32281 cgagacggtt tttcagcaac accaggcttt cgcgcgccac ttcacgcgct tctttgcggt
    32341 gcaggcggct ttcggcattg gtatccaccg ggtcagactc tttcggaccg agatggctgt
    32401 acgggtcgtt aaacaacccc atatcatatt taacgttcag tacatgacgg gtagcgtcat
    32461 ccagctcttc catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtagt
    32521 actcgtcgct catactcatg ttgatgccgg atttcagcgc cacgcgcacc gcatcttccg
    32581 ggtctgccgc cgtgccatgt ttaatcagct ctttgattgc gccgtgatcg gaaacggtga
    32641 tgcctttaaa gccccactgg tcgcgcagaa catctttcag cagccaggag tcggaggtgg
    32701 ctggcgtacc gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcctccagcc
    32761 ccgctttgta cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt
    32821 tgtactcttt accgccttct accgcgccgt aagcggcaaa gtgtttgacg ctggtcatca
    32881 ccgagtagcg atctgccgga cttttaccct gcatcgcttc caccatggtt ttccccatta
    32941 ttgaggtgag atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc
    33001 gcgagacatc gaccattggt gcccaggtca tattcaggcc atcatctgcc gcttcataag
    33061 cagagacacg cccgactgtt ttcaccgcat cgaggttaaa agacgaggcc agaccgaggc
    33121 taatcgggaa caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt
    33181 tcaggcggct taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg
    33241 tgttgaaaat cgccccaacc tgcccgtctt tgatcatctc gcggatcgcc tctttcgggt
    33301 tatccgggcc gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct
    33361 taagcagttc ggtgacgaac gcatcccgcg cttcaggcgt taatggatgg ttaccgaata
    33421 aatcatccgc cagtgctggc tgcagagcca gactcaccgc gattcctact gaacatagcc
    33481 atttcatatg gatttttctt cctttgttgc ccgacgtggc agcgaaaatg gtgcaaaaac
    33541 cgtagtttgc cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt
    33601 ttcaaaatat tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt
    33661 tcgccaccac aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt
    33721 gaatgaactt gcccgactgg tgggttcttc acacctgctc accgatcccg caaaaacggc
    33781 ccgctatcgc aagggcttcc gttctggtca gggcgatgca ctggctgtcg ttttccctgg
    33841 ctcactactg gaattgtggc gggtgctgaa agcctgcgtc accgccgaca aaattattct
    33901 gatgcaggca gccaatacag gtctgaccga aggctcgacg ccaaacggta acgactatga
    33961 tcgcgatatc gtgatcatca gcaccctgcg tctcgacaag ctgcacgttc ttggcaaggg
    34021 cgaacaggtg ctggcctatc cgggcaccac gctctattcg ctggaaaaag ccctcaaacc
    34081 gctgggacgc gaaccgcact cggtgattgg atcatcgtgt ataggcgcat cggtcatcgg
    34141 cggtatctgt aataactctg gtggttcgct ggtgcaacgt ggcccggcgt ataccgaaat
    34201 gtcgttattc gcgcgtataa atgaagatgg caagctgacg ctggttaacc atctggggat
    34261 tgatctgggc gaaacgccgg agcagatcct tagcaagctg gatgacgatc gcatcaaaga
    34321 tgacgatgtg cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg
    34381 tgatattgaa gccgacacgc ctgcacgtta taacgccgat ccggatcggt tatttgaatc
    34441 ttctggttgc gccgggaagc tggccgtctt tgcggtacgt cttgatacct tcgaagcgga
    34501 aaaaaatcag caggtgtttt atatcggcac caaccagccg gaagtactga ccgaaatccg
    34561 ccgtcatatt ctggctaatt tcgaaaatct gccggttgcc ggggaatata tgcaccggga
    34621 tatctacgat attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg
    34681 caccgacaag atgccgttct tctttaatct caaggggcgc accgatgcga tgctggagaa
    34741 agtgaaattc ttccgtccgc attttaccga ccgtgcgatg caaaaattcg gtcacctgtt
    34801 ccccagccat ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct
    34861 gttaaaaatg gcgggcgatg gcgtcggtga agccaaatcg tggctggtgg attatttcaa
    34921 acaggccgaa ggcgatttct ttgtctgtac gccggaggaa ggtagcaaag cgtttttaca
    34981 ccgtttcgcc gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga
    35041 agacattctg gcgctggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt
    35101 accgccggag atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta
    35161 tgtcttccat caggattaca tcgttaaaaa aggcgtggat gtgcatgcgt tgaaagaaca
    35221 gatgctggag ctgctacagc agcgcggcgc gcaataccct gccgaacata acgtcggtca
    35281 tttgtataaa gcaccggaga cgttgcagaa gttttatcgc gagaacgatc cgaccaacag
    35341 tatgaatccg gggatcggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa
    35401 attacggatg gcagagtatc gccatccgta attcacttaa tcgttctgtg ccgtctgccc
    35461 cgccgccgcc atttgggcgg ctttttgttt tttatagctc aacgctgctg ccggaacagg
    35521 catcacttta ccggtttcaa tccaggtacg caggcggctg gcatcggcaa aatgggtata
    35581 tttgccaaac gcatccatca ctaccagcgc caccggttta ttattgataa ccgtacgcat
    35641 caccagacaa tggcccgccg cattggtaaa gccggttttg gttagctgga tattccagtt
    35701 atcgcgatac accagatggt tagtattgcg gaacggcagc gtatacgtcg ggttagagaa
    35761 ggttgccata tcttcccggg tagtacttaa ctgcccgatc aacggatatt gtttgctggc
    35821 aatgagcagt ttggttaagt cacgggcagt tgaaacgtta tgcaccgata gtccggtcgg
    35881 ttcaacaaag cgcgtgttgt tcattccgag cgatttcgct ttcgcattca tcgccttaat
    35941 aaaggctttg taaccaccgg gataatggtg cgcaaggctt gccgccgcgc ggttttctga
    36001 agacatcagc gccagcaaca gcatatcttt acggctgatt tcgctgttca gtcgtacgcg
    36061 cgaatagacc cctttcatct ccggcgtctg gctgatatcc acttttagtt tttcatccag
    36121 cggcagtcgt gcatccagca cgaccatcgc ggtcattaat ttgctgatag acgcaatcgg
    36181 acgcaccaga tccgggtggt tcgaatagat cactttgttg gtattcagat caacaatcat
    36241 cgcgctaccg gaggcaattt ccggttgtga agcggtggta gcggctgccg ttttcgcaac
    36301 ggcctgcggt gcaaaaggca cagccagcat cagggccagg ctaaataaag aaactcgaaa
    36361 tttcggcatg atgagcattc atatagtggt tcacgcgcac gggttgcgca ccgccggagt
    36421 aaggatttac tgaggctagc gacgccatca taacgagcaa aaagtgcgat cgtcaaagga
    36481 gaatcgtgag gaaatgctgc attgctgaca tttacgccag caatgcaaca caaaagaact
    36541 ttctagaaca aacgataacc gtagccccat agtataacgg taagggcgag cagcacttcc
    36601 agtaccagca cgccaatcgc cagcgtcgaa ctggagaagc taagtccatc ctctttgttg
    36661 atattcagga agctcggaat accgaggtaa agcaggtagc cggtgtaaaa cagcgccacc
    36721 gtgccgacca gcgcacacaa ccagaccagc ggataaagcg ccaccagacc gcttaaaaac
    36781 agcggagttg caacgtagcc cgcaaagacc atgcagtgcg caagtgacgg acgctgcgga
    36841 taattgcgcg ccatccacca gatgacccgc cccatcaccg cgaccccagc cagcataacg
    36901 ccataaaaca agacagccag cgccagtccg gtaaaccagg ataacttcag gatagtgcca
    36961 tcgccaaaat tccagccaat ttgtgtggtg ccaatgaagg cgcaaatcac cggaatcgcc
    37021 gccatcagca aaacgtggtg ggtgtagtga tgagaaatcg tttcgttttc gcgattaatc
    37081 acctgcattt cacgatcggg atgggaaaac agtccccaga catggctcat accgccccct
    37141 tgttgtgagt tcatgaacct gacagttcaa gtataagtca gcttgtgatt attttttgtt
    37201 cgctcatgta atttgcaccg tctgatgtac gtcctttttc agcgtgaact attcacaggg
    37261 tgtatgctta caggcaagca aagggagaca gactggccta tggatctcaa tacactcatc
    37321 tcacaatatg gttatgccgc gctggtgatc ggtagcctgg cggaaggtga aaccgtgact
    37381 ttgctgggag gcgttgcggc acatcagggg ctattaaagt tcccgctggt ggtactttct
    37441 gtggcgcttg gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg gcgctttggc
    37501 ggcaagctat tacgccgttt ctcgaaacat caggataaaa ttgagcgggc gcagaaactt
    37561 atccaacgcc atccgtatct gtttgtcatt ggtacgcgtt ttatgtatgg ctttagggtg
    37621 attggcccga cgctgattgg tgccagccag ctgccgccga aaatctttct gccactgaat
    37681 atccttggcg catttgcctg ggcgttgatt tttaccacta tcggttacgc tggtggtcag
    37741 gtgattgcgc cgtggttgca caatctcgac cagcatttga agcactgggt ctggttgatt
    37801 ctggtcgtgg ttctggtggt aggcgtgcgc tggtggctga aacgacgcgg gaagaaaaag
    37861 ccggataatc aggcgtaaag cagttgccac agataagcat tcacgccaca tccggcaccg
    37921 ggtgttccat gcctgatgcg acactggcgt gtcttatctg gcctaaccca atgaaatatc
    37981 cacgcacctc actgcgctat tctgggttga actgcggatt cgccagcata aagccgccat
    38041 ccactatcaa cgactgccca gtggtgtaat ttgcgccctc cgaacaaagc cacaccacca
    38101 ggctggcaat ctcatgcgtt gcgccaaaac gccgcaaggg aatcgaaggc tccgcgccgg
    38161 gcttcacgtc gccatcatcc atgccgttca ttggcgtggc gatcgcccca ggtgcaaccg
    38221 cattcaccaa aatcttatgc ctgaccagct ccagcgccat cgctttggtt aacccgccga
    38281 gcgcatgttt agcggctgtg taggcgctgg catccggcaa cggcgtatgt tcatgcaccg
    38341 acgtaatgtt gatgatgcga ccaccctgcc cttgtttcac catctggcgg gcagcaattt
    38401 gcgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    38461 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    38521 gccccagccg ttgaatgagt ttctccagcg cctgcacacc ttctggcaga ttgccgagat
    38581 ccagttgcac gatctccgca cgtacgccgt ggctaaccac ctcacgcgcg gtatcttttg
    38641 ccccttcttc atcagagtgc caggtaatac caatatcaaa cccctgctgc gccagtaata
    38701 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcgacc tgtgccatcg
    38761 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcaacgtttg
    38821 taacccccgc ccaacgcacc agtgagttga atggaagcat ccagccactg cccttgcaat
    38881 aacaggccat tggcccgctc acgcagcgcc gggattctgg cttcgctgac ccgggaaccc
    38941 gcaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ctacacgcaa ggcatcgcgc
    39001 tcaatttgcg cctgatgctg gtttttctcc gccagtgtct gaacctgact agctgcccgc
    39061 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    39121 tcggcttttg cgatatcgag gttggcgtta agacggccac tatcgaaaat gggtagcgtc
    39181 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    39241 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    39301 tcaatagtgc ttaacgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    39361 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    39421 ggattaagct tcaatgcctt tgtctggtga tttgtaagtg cgcttaaccg tgcctcaata
    39481 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    39541 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtggcg
    39601 ataatggtgt tctgctcttt ttcgatttgc tgtaagacag tgtttaacgc cgcctgggtt
    39661 tgccactccc agtacagacg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    39721 tccgccgccc gtgctttaac cgtacccagg cgagcagtaa cctccgcccg attctttccc
    39781 cagatatcga gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacggaccg
    39841 gtcgtacctg cggccggatc gttcaaagca aacggcccca ttaacccttc tgccgacatt
    39901 ttttgccgct ccatatccgc agaaaagtcg agctgcggac catcctgagt ggcaactgcc
    39961 ttcgcctggg cttcagccag ttgaatgcgt tgttcagcca cctgcatatc cggtgcgttc
    40021 tgtagtgcgt tattaattaa tgaagtgagt tgattatcgt gatactccag ccaccattgg
    40081 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg cacagcgggc
    40141 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    40201 aacagcggaa aacaggcgat ggctggataa aaagaatcac gattcatggg ggaataatca
    40261 ggtaagaaaa ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    40321 caatacatca gtggcaataa aacaacatat tcagaaaaat atacactaag tgaatgatat
    40381 cttccgattt atcttaatcg tttatggata gcggcaaagg gcttcgtttt ttcctatact
    40441 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    40501 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    40561 ttaatgtcat cagatccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    40621 tgtcatgtgc attgcttcat ataatcactg gcgcaaggag cgcgcagggg gcgaccaatc
    40681 gccgccgccc cctgcacccc caggctctgg cgaacaaaat cgccgctgcg cggtgccctc
    40741 ggcttatccc ttacggctac cgggtcgggc gcgaggtaac atccctgtaa aacgcgccct
    40801 cagcccacat ccatgtgggc tgccccggcc ttcggggaac gcctcggcga ttttgacgcc
    40861 accaaacaac cgtgcggcct attgataaag agctaacaca ttgtcaaaaa acatcactat
    40921 ggttttttag agtttctcga tatcaattgc ctgaatagcc cttgcaatat caggggaatt
    40981 attcaacacc cgaacatgct gaaataattc cgttgcttca tcgtattctt tacgcaaata
    41041 actcaaccac tgtttaatcc gcgcaacgtg atataacccg gtatcgccct gcttttccag
    41101 acgggtatat ttttgcagca aagcaactac ctccggccac ggcattcgcg gttcgttata
    41161 ttttaccacc cggctcaggt tgggaatatt gagcgccccg cgaccaatca tcactgcgtc
    41221 gcatccgctg atcgtcatgc attgttgcgc actctgccag tcccagattt caccgttggc
    41281 aatcaccgga atattcagcc gctggcgaat ctcgccaatc gcctgccagt caatatgctc
    41341 cgcgcggtaa ccctgctctt tcgtccgccc atgcaccacc agctctgtag caccagcctg
    41401 ttgaaccgca tcggcgattt caaatttctt ctcaccgctg tcccatccca gacgcacttt
    41461 cacgctgacg ggcaaatgcg ccggtacagc ttcacgcatc gcttttgcac cctggtagat
    41521 gagttcggga tctttgagta acgtcgcccc accgccgcta ccgttaaccg ttttcgacgg
    41581 gcagccgcaa ttgagatcca cgccccagga acctaactcc accgcacggg cggcgttctc
    41641 tgccagccat tgtgggaact gacctaacaa ctgcacgcgc accagcgtac cggaaggtgt
    41701 ccggctggca ttttgtagct cagggcaaat gcgatgaaag acttttattg gcagcagttg
    41761 atccaccacg cggacaaact cggtgatgca cagatcgtag tcgttaactt cggtcagcaa
    41821 ttcacgcacc agagagtcga gtacaccctc catcggtgcc agtaacacac gcatatcatc
    41881 acccgcaaaa aaatgaggcg ctatgttagc gcctatggtc agcggattaa aggtctgcaa
    41941 agagggattg ctgcaaactg caatccctct ctatgcttga tgtatgcctg agttcgctgc
    42001 tcattgctta atgcccggga gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc
    42061 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    42121 gacactgaac attatctggc aatatttacg tgcttttgtc ctgatttatg cctgcctgta
    42181 tgcaggcatt ttcattgctt cactgctacc ggtaaccatt ccgggcagca tcatcgggat
    42241 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcaaaatggg tcaatccggg
    42301 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    42361 gcaatatttt gatttgctcc gcgcacagtt tggcccggta gtggtttcct gtgcagtcag
    42421 tacgctggtg gtttttctgg tggtgagctg gagttcgcaa ctggtgcacg gtgaacgtaa
    42481 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    42541 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccttgctt
    42601 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac cggcatctct
    42661 tacgacagct actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    42721 gcgctggcct atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    42781 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc tgtggcattg
    42841 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct
    42901 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtc
    42961 attttcgtcg gcatcctcgg cgctgtgttt ggccatacat tgcttaatgc gatgcgtatt
    43021 cgtaccaaag ctgcgcgcgg tctggcgatg gggacagcct cgcacgccct cggtacggcg
    43081 cgctgcgccg agctggatta tcaggaaggt gcgtttagtt cgctggcact ggtgttatgc
    43141 gggataatta cttcgctgat cgcaccgttc cttttcccga ttattctggc agtaatgggc
    43201 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    43261 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    43321 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    43381 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    43441 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    43501 cttccgctgg cggcggcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    43561 gcgcgcggtg tgagcggaac ctggtatttt ggtgccaata tggaatttat tggtgcgaca
    43621 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa
    43681 aaagcgcttg cagcgatcac cgttaactac acgccttgtg gtcactgccg tcagtttatg
    43741 aatgaactga acagcggtct ggatctgcgt attcatcttc cgggacgcga ggcacacgcg
    43801 ctgcgtgact atctaccgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    43861 atggacgagc aggatcacgg ctatgcgctg acgggtgatg cgctttctca ggcggcgatt
    43921 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    43981 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    44041 ctgccaccgt tgcagggggc gttaattctg ttgaatctca agggttatga ttacccggat
    44101 atccagcgcg cggttctggc agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc
    44161 tccgcaacgc tgaaagctct cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc
    44221 tggtgccgga tgcggcgtga acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa
    44281 atcccggcaa acagtttgcc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    44341 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    44401 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    44461 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg
    44521 gcacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    44581 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    44641 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg
    44701 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    44761 cacgcaaagt tttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    44821 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    44881 cgaaggatat gctgtcagta cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg
    44941 acctttatat ttttaaacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    45001 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacgcccgaa cagttacttg
    45061 aattgcaaaa gaaacaagga aagtagttat ggatgtacag catttttttg tcgttgccgt
    45121 ttttttcctc attccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    45181 gattgataaa cgggttaaaa atgcaccgga accggtgtat gtctggcgag caaaaaatcc
    45241 cggactcttc ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    45301 gattgtttat cttattttct atcgttaata ttttctcttt tctgt
//
LOCUS       DARNVM010000016        43533 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.016, whole genome shotgun sequence.
ACCESSION   DARNVM010000016 DARNVM010000000
VERSION     DARNVM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.016"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            128..1222
                     /gene="gcvT"
                     /locus_tag="SI621_000886"
     CDS             128..1222
                     /gene="gcvT"
                     /locus_tag="SI621_000886"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEI4063631.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1246..1635
                     /gene="gcvH"
                     /locus_tag="SI621_000887"
     CDS             1246..1635
                     /gene="gcvH"
                     /locus_tag="SI621_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEI4063632.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEAGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1754..4627
                     /gene="gcvP"
                     /locus_tag="SI621_000888"
     CDS             1754..4627
                     /gene="gcvP"
                     /locus_tag="SI621_000888"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEI4063633.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTDQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAGEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4793..6226)
                     /gene="bglA"
                     /locus_tag="SI621_000889"
     CDS             complement(4793..6226)
                     /gene="bglA"
                     /locus_tag="SI621_000889"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HEI4063634.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            6271..6582
                     /locus_tag="SI621_000890"
     CDS             6271..6582
                     /locus_tag="SI621_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEI4063635.1"
                     /translation="MQPNDITFFQRFQDDILAGRKIITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6746..7405
                     /locus_tag="SI621_000891"
     CDS             6746..7405
                     /locus_tag="SI621_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEI4063636.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(7482..8462)
                     /gene="ygfZ"
                     /locus_tag="SI621_000892"
     CDS             complement(7482..8462)
                     /gene="ygfZ"
                     /locus_tag="SI621_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HEI4063637.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8705..8971
                     /gene="sdhE"
                     /locus_tag="SI621_000893"
     CDS             8705..8971
                     /gene="sdhE"
                     /locus_tag="SI621_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HEI4063638.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            8952..9359
                     /locus_tag="SI621_000894"
     CDS             8952..9359
                     /locus_tag="SI621_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEI4063639.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9399..9920)
                     /gene="fldB"
                     /locus_tag="SI621_000895"
     CDS             complement(9399..9920)
                     /gene="fldB"
                     /locus_tag="SI621_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HEI4063640.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10032..10928
                     /gene="xerD"
                     /locus_tag="SI621_000896"
     CDS             10032..10928
                     /gene="xerD"
                     /locus_tag="SI621_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEI4063641.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            10953..11663
                     /gene="dsbC"
                     /locus_tag="SI621_000897"
     CDS             10953..11663
                     /gene="dsbC"
                     /locus_tag="SI621_000897"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HEI4063642.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11669..13402
                     /gene="recJ"
                     /locus_tag="SI621_000898"
     CDS             11669..13402
                     /gene="recJ"
                     /locus_tag="SI621_000898"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEI4063643.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13493..14591
                     /gene="prfB"
                     /locus_tag="SI621_000899"
     CDS             join(13493..13567,13569..14591)
                     /gene="prfB"
                     /locus_tag="SI621_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEI4063644.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14601..16118
                     /gene="lysS"
                     /locus_tag="SI621_000900"
     CDS             14601..16118
                     /gene="lysS"
                     /locus_tag="SI621_000900"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEI4063645.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16161..16709)
                     /gene="idi"
                     /locus_tag="SI621_000901"
     CDS             complement(16161..16709)
                     /gene="idi"
                     /locus_tag="SI621_000901"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEI4063646.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16764..16834
                     /locus_tag="SI621_000902"
                     /pseudo
     CDS             16764..16834
                     /locus_tag="SI621_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16831..16956)
                     /locus_tag="SI621_000903"
     CDS             complement(16831..16956)
                     /locus_tag="SI621_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063647.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(16958..18406)
                     /gene="uacT"
                     /locus_tag="SI621_000904"
     CDS             complement(16958..18406)
                     /gene="uacT"
                     /locus_tag="SI621_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HEI4063648.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18842..20761
                     /gene="ygfT"
                     /locus_tag="SI621_000905"
     CDS             18842..20761
                     /gene="ygfT"
                     /locus_tag="SI621_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HEI4063649.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20761..21249
                     /locus_tag="SI621_000906"
     CDS             20761..21249
                     /locus_tag="SI621_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEI4063650.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(21285..22652)
                     /gene="ghxQ"
                     /locus_tag="SI621_000907"
                     /gene_synonym="ygfQ"
     CDS             complement(21285..22652)
                     /gene="ghxQ"
                     /locus_tag="SI621_000907"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HEI4063651.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22688..24004)
                     /gene="guaD"
                     /locus_tag="SI621_000908"
     CDS             complement(22688..24004)
                     /gene="guaD"
                     /locus_tag="SI621_000908"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEI4063652.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24022..25422)
                     /gene="xanQ"
                     /locus_tag="SI621_000909"
     CDS             complement(24022..25422)
                     /gene="xanQ"
                     /locus_tag="SI621_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HEI4063653.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVILVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25587..28457)
                     /locus_tag="SI621_000910"
     CDS             complement(25587..28457)
                     /locus_tag="SI621_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HEI4063654.1"
                     /translation="MIIHFTLNGALQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28454..29233)
                     /locus_tag="SI621_000911"
     CDS             complement(28454..29233)
                     /locus_tag="SI621_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HEI4063655.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALCEALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29284..30612)
                     /gene="ssnA"
                     /locus_tag="SI621_000912"
     CDS             complement(29284..30612)
                     /gene="ssnA"
                     /locus_tag="SI621_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HEI4063656.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIDDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30615..33713)
                     /gene="ygfK"
                     /locus_tag="SI621_000913"
     CDS             complement(30615..33713)
                     /gene="ygfK"
                     /locus_tag="SI621_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HEI4063657.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQNEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLSDVFMIGDIQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34035..34613)
                     /gene="mocA"
                     /locus_tag="SI621_000914"
     CDS             complement(34035..34613)
                     /gene="mocA"
                     /locus_tag="SI621_000914"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HEI4063658.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDI
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34717..35487
                     /gene="yqeC"
                     /locus_tag="SI621_000915"
     CDS             34717..35487
                     /gene="yqeC"
                     /locus_tag="SI621_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HEI4063659.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWLVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGEKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            35535..37160
                     /locus_tag="SI621_000916"
     CDS             35535..37160
                     /locus_tag="SI621_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HEI4063660.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37201..38133)
                     /gene="arcC"
                     /locus_tag="SI621_000917"
     CDS             complement(37201..38133)
                     /gene="arcC"
                     /locus_tag="SI621_000917"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEI4063661.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38181..39566)
                     /gene="hyuA"
                     /locus_tag="SI621_000918"
     CDS             complement(38181..39566)
                     /gene="hyuA"
                     /locus_tag="SI621_000918"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HEI4063662.1"
                     /translation="MRVLIKNGIVANADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39619..40830)
                     /locus_tag="SI621_000919"
     CDS             complement(39619..40830)
                     /locus_tag="SI621_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEI4063663.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40888..42084)
                     /gene="dpaL"
                     /locus_tag="SI621_000920"
     CDS             complement(40888..42084)
                     /gene="dpaL"
                     /locus_tag="SI621_000920"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEI4063664.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42142..43329)
                     /gene="ygeW"
                     /locus_tag="SI621_000921"
     CDS             complement(42142..43329)
                     /gene="ygeW"
                     /locus_tag="SI621_000921"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HEI4063665.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10556 a        10942 c        11467 g        10568 t
ORIGIN      
        1 ttcggtggta agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat
       61 gctgggtttc gtggtgataa tttcgccata gaaaagttgt cagccccgct tattcaatga
      121 ggacaagatg gcacaacaga ctcctttgta cgaacaacac acgctttgcg gcgctcgcat
      181 ggtggatttc cacggctgga tgatgccgct gcattacggt tcgcaaatcg acgaacatca
      241 tgcggtacgt accgatgccg gaatgtttga tgtgtcacat atgaccatcg tcgatcttcg
      301 cggcagccgc acccgggagt ttctacgtta tctgctggca aacgatgtgg cgaagctcac
      361 caaaagcggc aaagcccttt actcggggat gttgaatgcc tctggcggtg tgatagatga
      421 tctcatcgtc tactacttta ctgaagattt cttccgcctc gttgttaact ccgccacccg
      481 cgaaaaagac ctctcctgga ttacccaaca cgctgaacct ttcggcatcg aaattactgt
      541 tcgtgatgac ctttccatga tcgccgtaca agggccgaat gcgcaggcaa aagctgccac
      601 actgtttaat gacgcccagc gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca
      661 ggcgggcgat ctgtttattg ccaccaccgg ttacaccggt gaagcgggct acgaaattgc
      721 gctgcccaat gaaaaagcgg ccgatttctg gcgcgcgctg gtggaagctg gcgttaagcc
      781 atgtggcctg ggcgcgcgtg acacgctgcg tctggaagcg ggcatgaatt tgtatagcca
      841 ggagatggac gaaactattt ctcctttagc cgccaatatg ggctggacta tcgcctggga
      901 accggcagat cgtgacttta tcggtcgtga agctctggaa gcgcagcgtg aacatggcac
      961 agaaaaactg gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt
     1021 acgttttact gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg gtactttctc
     1081 cccaacgctg ggttatagca ttgcgctggc gcgcgtgccg gaaggtattg gtgaaacggc
     1141 gattgtgcaa attcgcaacc gtgaaatgcc ggttaaagtg acgaaacctg tttttgtgcg
     1201 taacggcaaa gccgtcgcgt gatttacttt tttggagatt gattgatgag caacgtacca
     1261 gcagaactga aatacagcaa agaacacgaa tggttgcgta aagaagccga cggcacttac
     1321 accgttggca tcaccgaaca cgctcaggag ctgttaggcg atatggtgtt tgttgacctg
     1381 ccggaagcgg gcgcaacggt tagcgcgggt gatgactgcg cggttgccga atcggtaaaa
     1441 gcggcgtcag atatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgatgcactg
     1501 agcgattctc cggaactggt gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc
     1561 aaagccagcg atgaaagcga actggaatca ctgctggatg caaccgcata cgaagcattg
     1621 ttagaagacg agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggaacat
     1681 ttacgcctca ccctcactct cttcgtaagg agagaggttc acaattcact gcacgtttca
     1741 ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aacagcggcg cttttattga
     1801 acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg ttggcgcaca
     1861 atcgttaaac gcgctgaccg accagattgt gccgaaagat attcagcttg cgactccgcc
     1921 acaggttggc gcaccggcga ccgaatacgc cgcactggca gaactcaagg ctattgccag
     1981 tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc agctaccgcc
     2041 ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtata ctccgtatca
     2101 acctgaagtc tcccagggcc gccttgaagc actgctcaac ttccagcagg taacgctgga
     2161 tttaaccggg ctggatatgg cctctgcttc tcttcttgac gaagccaccg ctgccgccga
     2221 agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct tctttgttgc
     2281 ttctgacgtt catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa cctttggttt
     2341 tgaagtgatt gtcgatgacg cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct
     2401 gttacagcag gttggtacta ctggtgaaat tcacgactac accgcgctta ttagcgaact
     2461 gaaatcacgc aaaattgtgg tcagcgtcgc cgccgatatt atggcgctgg tgctgttaac
     2521 tgcgccgggt aaacagggcg cagatattgt ctttggttcg gcgcaacgct tcggcgtgcc
     2581 gatgggctac ggtggcccac acgcggcatt ctttgcggcg aaagatgaat acaaacgctc
     2641 aatgccgggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat
     2701 ggcaatgcag actcgcgagc aacatattcg ccgtgagaaa gcgaactcca acatttgtac
     2761 ttcccaggta ctgctggcaa acatcgccag cctgtatgcc gtttatcacg gtccggttgg
     2821 cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg cgggcctgca
     2881 acaaaaaggt ctgaagctgc gccatgcgca ctatttcgat actttgtgtg tggaagtggc
     2941 cgacaaagcg ggcgtgctgg cgcgtgccga agcgggtgaa atcaacctgc gtagcgatat
     3001 tctgaacgcg gttgggatca cccttgatga aacaaccacg cgcgaaaacg tgatgcagct
     3061 tttcagcgtg ctgttgggcg ataaccacgg gctggacatc gacacactgg acaaagacgt
     3121 ggctcacgac agtcgctcta ttcaggctgc gatgctgcgc gacgacgaaa tcctcaccca
     3181 tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc actcgctgga
     3241 gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca ccatgaaact
     3301 gaacgccgcc gccgagatga tcccgatcac ctggccggaa tttgccgaac tgcacccgtt
     3361 ctgcccgccg gaacaggctg aaggttatca gcagatgatc gcgcagctgg ctgactggct
     3421 ggtgaaactg accggttacg acgccgtttg tatgcagccg aactctggcg cacagggcga
     3481 atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga
     3541 tatctgcctg atcccggcct ctgctcacgg cactaacccg gcttccgcgc atatggcagg
     3601 aatgcaggtg gtggttgtgg cgtgtgataa aaacggcaac atcgatctga ctgatctgcg
     3661 cgcgaaagcg gagcaggcgg gcgataacct ctcctgcatc atggtgacct atccttccac
     3721 ccacggcgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg
     3781 tcaggtttac cttgatggcg cgaacatgaa cgctcaggtt ggcatcacct cgccgggctt
     3841 tattggtgcg gacgtttccc accttaacct gcataaaact ttctgcattc cgcatggcgg
     3901 tggtggtccg ggtatgggac cgatcggtgt gaaagcgcat ctggctccgt ttgtaccggg
     3961 tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcggttt ctgcggcacc
     4021 gttcggtagt gcctctatcc tgccaatcag ctggatgtac atccgcatga tgggcgcaga
     4081 agggctgaaa aaagcaagcc aggtggcaat tcttaacgcc aactatattg ccagccgtct
     4141 gcaggatgcc ttcccggtgc tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat
     4201 tctcgatatt cgcccgctga aagaagaaac cggcatcagc gagctggata ttgccaagcg
     4261 cctgatcgac tacggtttcc acgcgccgac catgtcgttc ccggtggcgg gtacgctgat
     4321 ggttgaaccg actgaatctg aaagcaaagt ggaactggat cgctttattg atgcgatgct
     4381 ggctatccgc gcagaaattg atcaggtgaa agccggtgtc tggccgctgg aagataaccc
     4441 gctggtgaat gcgccgcaca ttcaaagcga actggtcgcc gagtgggcgc atccgtacag
     4501 ccgtgaagtt gcagtattcc cggcaggtgt ggcagacaaa tactggccga ccgtgaaacg
     4561 tctggatgat gtttacggcg accgtaacct gttctgctcc tgcgtaccga ttagcgaata
     4621 ccagtaattt actgattcga ctatcttata aaggcgcttc ggcgcctttt tcgcttgtta
     4681 ataaagtgcg ctttgtgtgt ttcggctgag gcgtaatata aaatattgcg agctcaatat
     4741 attaaaagag tgccaggcct gatgagcgga gcacatcagg cacatcggct aattacagat
     4801 tctcgccgtt gctggcaatc acctctttgt accagtcaaa gctcttctta cgtgaacgcg
     4861 acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt
     4921 actgcccggt ggtgaacgac acacagtcga tgcagcccca cggcgtgtag cccatcaggt
     4981 ccacgccatc gtaagtcacc gcttttttca tctcttcaat atgggcgcgc aggtagtcaa
     5041 tgcggtaatc gtcattgatg ctgccatcat cttccacttt gtcgtaagcg ccaaaaccgt
     5101 tttcgacaat aaacagcggc ttctggtaac gctcatacag ctcgcaaagc gcgtaacgca
     5161 gaccaaccgg atcaatctgc cagccccagt cagacgcttt aacatacggg ttcggtacgc
     5221 tgccttcaaa accagagatc gcatcgccgg tgccgccttc ggccttcacc gcgttggtca
     5281 tgtaatagct gaagccgaga taatcgcagg tgccttcgcg cagtacatcc agatcgccgt
     5341 cttccatttt gatgttaaat ccgcggcgtt cccactcgtt caacacatag gacgggtaat
     5401 agccacgcaa ctgcacatcg gtaaagacgt aacgttcgcg catcgactcc tgggcaaaca
     5461 tcacatcgtc cgggttacag gagtaaggat agagcggcac catcgccagc atacaaccga
     5521 ctttcatctc cgggttgatg cgatgcgcgg ctttcaccgc cagggcgctg gcgacaaact
     5581 ggtgatgcag cacctgatac atcgtctctt ccgggttttc atgctcggta tacaccacgc
     5641 cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta atttcgttga
     5701 aggtcatcca gtatttgact ttatgcttat agcgttcaaa taccacttcc gcgaaacgga
     5761 caaagaaatc aaccacttta cggttggtcc agctaccata ttgctgcacc agatgcagcg
     5821 gcatttcaaa gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc agttcatcga
     5881 tcatatcatc gtagaacttc agcccttctt cgtttggctg agcttcatcg ccttttggaa
     5941 aaatgcgcgt ccaggcgatg gaagtacgaa aacatttgaa gcccatttcg gcaaatagct
     6001 tgatgtcctc cttgtagtga ccataaaaat caacggcttc atggtttgga tagtatttgc
     6061 caggcacaac ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg gtcaaaacgt
     6121 cacaaatgct cggccctttg ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa
     6181 ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca
     6241 gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac tttttttcaa
     6301 cgtttccagg atgacattct ggctgggcgt aaaatcatta ccatccgcga cgagtctgaa
     6361 tcgcacttca aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt
     6421 tgcacgattg aagtcacggc aacctcaacc gtaacgctgg atacgctgac ggaaaaacat
     6481 gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga catctatccc
     6541 gaccagacac agttttatgt gattgaattt aaatgtcttt aaattgaact cacgcctgtt
     6601 agttgaaatt tcgcatttaa tttcatgatt tttcggagat taatctcaaa tcagaattta
     6661 ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg tatacatgtg
     6721 cgtgtacgtt tttccggagt aagttatggt tcagaagccc ctcattaagc agggatattc
     6781 gctggcagag gaaattgcca acagcgtcag tcacggcatt gggctggtgt ttggtatcgt
     6841 tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca cggcgataac
     6901 cagttacagc ctctatggag gcagtatgat cctgctgttc ctcgcttcga cgctctatca
     6961 cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccact gcgctattta
     7021 tctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt ctccgttagc
     7081 gcgcgggttg atgattgtta tctggagcct ggcattgctg ggtattctgt ttaaactgac
     7141 catcgcgcac cgattcaaaa ttttatctct ggtgacctat ctggcgatgg gctggctgtc
     7201 gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta ccttactggc
     7261 ggtaggcggt gtggtttatt cgctcggggt gattttctac gtctgcaagc gcattccata
     7321 caatcatgcc atctggcacg gctttgtgct cggcggtagt gtgtgccact ttctggcgat
     7381 ctatttgtat attgggcagg tgtaattggc gtgcctttgt aggcctgaaa agacacacca
     7441 gtgtcgcatc aggcgataaa tgcctgatgc gatacagaga tttactcttc cagcgaatat
     7501 ggcagtggct caatacgcaa tgtattcgca tcgtcacgca cgcggaagat gctgtccggt
     7561 tccatatcgt tattcatgac aacctgcacc acaacctgac catcttccag tttgaccgca
     7621 gccagcaccg tacctgtacg acgccagttc tcgcccattt tcagctctaa gtcttcacca
     7681 gcttccggca gtcggctggc gctacccttc agcaaccaga gcgcgcgttt gttggcacca
     7741 cggaattttg ctcgcgccac catctcttgt ccggtatagc agcctttctt aaagctgata
     7801 ccgcccagcg cctgaaggtt ggtcgcctgg gggataaact gcccgctgtt ggcggcatca
     7861 atcaccggaa aacccgcttc aatgtttaat gccagccact gttggctatt gttcagttcc
     7921 gcttcaccgc gcagtttatc ggtcagcata ttggcagtag cttcatcggt tacgatcagg
     7981 aaacgttctg ccgggtgttc aaaccatagc aacgtggtcg cgccttcttt gactacctgt
     8041 ttttctctcg aaggtagttc actaaagaga tttgccaacg cggcgcgcgc ctgaaaaccg
     8101 gcaacaccaa gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga gaacaccgca
     8161 tattttttca gttcagtcag ctgcggttca cgcacgctgc gccgttcaat ccatgcaaag
     8221 ccatcgccgt cgcggaacag acgtaaattg ctccacattt tacctttggc gtcgcaatgg
     8281 gcggcgagca ggtgctgatc ttcagtcatc tggctgacat ctgctgtcac ctggccttgc
     8341 atatattttt cgctgtccgc gccagtaata gtggcaagcg cccagtcatc aagcgtcatc
     8401 agcgtcagtg gcaaacgggc agaagccgta ggctgacggg gaggaaaagg tgtaaaagcc
     8461 ataatagagt cctgagttgc ttaacggcgt attaatcact aatggtaaaa gagctatgag
     8521 gcaatgcaag tgctttcact gcgcgatctg tgccttctgc gaggcgactt ccaaaaaaat
     8581 gaagcccttg aattgttgtg aattaatttc gcgatcgcga ttgtgtttac tgatattccc
     8641 gtaaaagaag tcgaaaaaca cgttacaata gccgggtact ggattttcgt gagacaggaa
     8701 gaagatggac attaacaaca aagcccgcat tcattgggca tgccgccgtg gtatgcgcga
     8761 actcgatatt tcaatcatgc cgtttttcga acatgagtac gacagcttaa gcgatgacga
     8821 aaaacgcatc tttattcgtc tgctggaatg tgacgatccg gacctgttta actggctgat
     8881 gaatcacggt aaaccggccg atgcagaact ggaaatgatg gtccgactca tccagacacg
     8941 gaaccgggaa cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc gcgcagtggc
     9001 tttccttgct gatccacggg ctggttgccg ctgttatttt actcatgccc tggccgctca
     9061 gttacacccc gttatggatg gtgttacttt cgctggtggt gtttgattgc gttcgcagcc
     9121 agcgacgcat taatgctcgc cagggggaaa ttcgcctgtt gatggacggg cgtttgcgtt
     9181 ggcaagggca ggagtggagc atcgtcaaag cgccgtggat gattaagagc ggcatgatgc
     9241 tgcgtttacg ttctgacagc ggaaaacggc aacatttatg gctggcagct gacagcatgg
     9301 acgaagccga atggcgggat ttacggcgga tattgttaca acaagagacg caaagataaa
     9361 gatgacggca aagcatatgt gctaagccgt cagtgacatc aggcgtaatg ctctgccatt
     9421 tcgttgagga tttgctcgca ccagctctga atacgctcgt cgctaaggtc atactggtta
     9481 gtttcatcca gcgccagacc cacgaacagt tgcccgtcag caatcaccgg tttcgggctg
     9541 gtaaattcat atccttccgt tggccagtag ccgacgaact tcacgccttt ggtcgagagt
     9601 ttgtcatgca gcataccgag cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca
     9661 agcccataca gcgcaacaat tttaccttcg aggttcaggt cgtcgagctg atcccagacg
     9721 gcttcccagt cttcctggat ttcgccaaaa tcccaggtcg ggatacccag aatgagcaca
     9781 tcgtactgct ccattaattt cggggagtcg tccttgaggt tatgtaaggt caccagttct
     9841 gggccgataa tatcgcggat tttttctgcc gccatttcgg tgtaacaggt gctggaaccg
     9901 taaaaaagac ccatattcat aacgtaagct tctcgattct gctgtggctt ttgcggctag
     9961 tgtatcagaa tcgctttagt cacggcacaa taaggcataa tggatcaaat gagtgaccat
    10021 aaggggcgca agtgaaacag gatctggcac gcatcgagca gtttcttgat gctctgtggc
    10081 tggaaaaaaa tctggctgaa aatacgttga acgcttaccg tcgcgatctg tcaatgatgg
    10141 tggagtggtt gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac gatttgcagg
    10201 cattactggc agaacggctg gagggcgggt ataaagccac cagctcagcg cgtttgctga
    10261 gtgcagtgcg ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa gatgatccca
    10321 gtgcgcatct cgcttcaccg aaattgcccc agcgtttgcc aaaagattta agcgaagcgc
    10381 aggtcgaacg tttattacag gcaccattaa ttgatcagcc actggagcta cgcgataaag
    10441 ccatgcttga agtgttgtat gctaccggac tgcgtgtctc tgaactggtc gggctgacaa
    10501 tgagtgatat cagcctgcgt cagggcgtgg tgcgggtcat tggtaaaggc aacaaagagc
    10561 gcctggtgcc gttaggtgaa gaggcggttt actggctgga aacctatctg gaacatgggc
    10621 gtccgtggct gttgaatggt gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc
    10681 agatgacgcg acagaccttc tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg
    10741 acagcgaaaa gctgtcaccg catgtgttgc gtcacgcttt tgccactcac ttattaaatc
    10801 atggtgcgga tttacgcgtg gtgcagatgc tactgggcca cagcgatctc tccaccacgc
    10861 aaatttatac gcatgtcgct accgagcgtc tgcgacaact tcatcaacag catcacccgc
    10921 gggcgtgatg tctgaaaaga acaggaaaat ttatgaagaa aggttttatg ttatttacct
    10981 tgttggcagc gttttcaggc tttgttcagg ctgatgacgc ggcaattcaa caaacgttag
    11041 ccaaaatggg tatcaaaagt agcgatattc agcccgcgcc cgtagctggc atgaagacag
    11101 ttctgactaa cagcggtgtg ttgtacatca ccgatgatgg taaacatatc attcaggggc
    11161 caatgtatga cgttagtggc acggctccgg tcaatgtcac caataagatg ctgttaaagc
    11221 agttgaatgc gcttgaaaaa gagatgatcg tttataaagc gccgcaggaa aaacacgtca
    11281 tcaccgtgtt tactgatatt acctgtggtt actgccacaa actgcatgag caaatggcag
    11341 actataacgc gctggggatc accgtgcgtt atcttgcttt cccgcgccag gggctggaca
    11401 gcgatgcaga gaaagaaatg aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg
    11461 atgatgtgat ggcaggtaaa agcgtcgcac cagccagctg cgacgtggat attgccgacc
    11521 attacgcact tggcgtccag cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg
    11581 gcacacttgt tccgggttac cagccgccga aagacatgaa agaattcctc gacgaacacc
    11641 aaaaaatgac cagcggtaaa taattcgcgt gaaacaacag atacaacttc gtcgccgaga
    11701 agtcgatgaa acggccgact tgcccgctga attgcctccc ttgctgcgcc gtttatatgc
    11761 cagccggggt gtgcgtagcg cgcaggaact ggaacgcagt gttaaaggca tgctgccctg
    11821 gcagcaactg agcggcgtcg aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg
    11881 aacgcggatt attgtggtcg gtgatttcga cgccgacggg gccaccagca cggctctaag
    11941 cgtgctggcg atgcgctcgc ttggttgcag caatatcgac tacctggttc caaaccgttt
    12001 cgaagacggt tacggcttaa gtccggaagt agtcgatcag gctcatgccc gtggcgcgca
    12061 gttaattgtc acggtggata acggtatttc ctcccatgcg ggggttgaac acgctcgttc
    12121 gttgggcatt ccggttattg ttaccgatca ccatttgcca ggcgacacat tacccgcagc
    12181 ggaagcgatc attaacccta acttgcgcga ctgtaatttc ccgtcgaaat cactggcggg
    12241 cgttggcgtg gcgttttatc tgatgctggc gttacgcact tttttgcgcg atcagggctg
    12301 gtttgatgaa cgcggcatcg caattcctaa tctggcagaa ctgctggatc tggtagcgtt
    12361 ggggacagtg gcggacgtcg tgccgctgga cgctaataat cgcattctga cctggcaggg
    12421 gatgagtcgc atccgtgccg gaaagtgccg tccggggatt aaagcgttgc tggaagtggc
    12481 caatcgcgat ccacaaaaac tcgccgccag cgatttaggt tttgcgctgg ggccacgtct
    12541 caatgctgcc gggcgactgg acgatatgtc cgttggcgtg gcgctgttgc tgtgcgacaa
    12601 catcggcgaa gcgcgcgtgc tggcaaatga actcgatgcg ctaaaccaga cgcgaaaaga
    12661 gatcgaacag ggaatgcaag ttgaagccct gaccttgtgc gagaaactgg agcgcagccg
    12721 cgacacgcta cccggtgggc tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg
    12781 tattctggct tcgcgcatca aagagcgttt tcatcgtccg gttatcgcct ttgcgccagc
    12841 tggtgatggt acgctgaaag gttccggtcg ctccattcag gggctgcata tgcgtgatgc
    12901 gctggagcgg ctagacacgc tttaccccgg catgatgctc aagtttggcg gtcatgcgat
    12961 ggcggcgggt ttgtcgctgg aagaggagaa attcgaactc tttcaacaac ggtttggcga
    13021 actggttact gagtggctgg acccttcgct attgcaaggc gaagtggtgt cggacggtcc
    13081 gttaagtccg gccgaaatga ccatggaagt ggcgcagctg ttgcgcgatg ctggcccgtg
    13141 gggacaaatg ttcccggaac cgttgtttga cggtcatttc cgtctgttgc aacagcggct
    13201 ggtgggcgag cgtcatttga aggtgatggt cgaaccggtc ggcggcggtc cgctgctgga
    13261 cgggattgct tttaatgtcg ataccgccct ctggccggat aacggcgtgc gcgaagtgca
    13321 actggcttac aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat
    13381 cgacaatatc tggccaattt agcgtcatct tctctataaa aaagagcgtg gattgggtac
    13441 aatcccgctc ttatcaccgc attttgacta gctcaataaa agaaatcaga ccatgtttga
    13501 aattaatccg gtaaataatc gcattcagga cctcacggaa cgctccgacg ttcttagggg
    13561 gtatctttga ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc gagctggaac
    13621 agccggatgt ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag cgttcctccc
    13681 tcgaagccgt tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc
    13741 tgctggaact ggctgtagaa gctgacgacg aagaaacctt taacgaagcc gttgctgaac
    13801 tcgacgccct ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat
    13861 atgacagcgc cgactgctac ctcgatattc aggcggggtc tggcggtacg gaagcacagg
    13921 actgggcgag catgcttgag cgtatgtatc tgcgctgggc agaatcgcgt ggtttcaaaa
    13981 ctgaaatcat cgaagagtcg gaaggtgaag tggcgggtat taaatccgta acgatcaaaa
    14041 tctccggcga ttacgcttat ggctggctgc gtacagaaac cggcgttcac cgcctggtgc
    14101 gtaagagccc gtttgactcc ggcggtcgtc gccacacgtc gttcagctcc gcgtttgttt
    14161 acccggaagt tgatgatgat atcgatatcg aaatcaatcc ggcagatctg cgcatcgacg
    14221 tttatcgcgc gtccggcgcg ggcggtcagc acgttaaccg taccgaatct gcggtgcgta
    14281 ttactcacat cccgaccggg atcgtgactc agtgccagaa cgaccgttcc cagcacaaga
    14341 acaaagacca ggccatgaag cagatgaaag cgaagcttta tgaactggag atgcagaaga
    14401 aaaatgccga gaaacaggcg atggaagaca ataaatccga catcggctgg ggcagccaga
    14461 ttcgttctta tgtccttgat gactcccgca ttaaagatct gcgtaccggg gtagaaaccc
    14521 gcaacacgca ggccgtgctg gacggcagcc tggatcaatt tatcgaagca agtttgaaag
    14581 cagggttatg aggaaccaac atgtctgaac aacacgcaca gggcgctgac gcggtagtcg
    14641 atcttaacaa tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgt gagcagggga
    14701 ttgccttccc gaacgatttc cgtcgcgatc atacctctga ccaattgcac gcagaattcg
    14761 acggtaaaga gaacgaagaa ctggaagcgc tgaacatcga agtcgccgtt gctggccgca
    14821 tgatgacccg tcgtattatg ggtaaagcgt ctttcgttac cctgcaggac gttggcggtc
    14881 gcattcagtt gtacgttgcc cgtgacgatc tgccggaagg cgtttacaac gagcagttca
    14941 aaaaatggga cctcggcgat atcctcggcg cgaaaggtaa actgttcaaa accaaaaccg
    15001 gcgaactgtc tatccactgt accgagctgc gtctgctgac caaagcactg cgtccgctgc
    15061 cggataaatt ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt tatctggatc
    15121 tcatctctaa cgatgaatcc cgcaacacct ttaaagtgcg ctcgcagatc ctctctggta
    15181 ttcgccagtt catggtgaat cgcggcttta tggaagttga aacgccgatg atgcaggtga
    15241 tccctggcgg tgccgctgcg cgtccgttta tcactcacca taacgcgctg gatctcgaca
    15301 tgtacctgcg tatcgcgccg gaactgtacc tcaagcgtct ggtggtcggt ggcttcgagc
    15361 gtgtattcga aatcaaccgt aacttccgta acgaaggtat ttccgtacgt cataacccag
    15421 agttcaccat gatggaactc tatatggctt acgcagatta caaagatttg atcgagctga
    15481 ccgaatcgct gttccgtact ctggcacagg atattctcgg taagacggaa gtgacctacg
    15541 gcgacgtaac gctggacttc ggtaagccgt tcgaaaaact gaccatgcgt gaagcgatca
    15601 agaaatatcg cccggaaacc gacatggcag atctggacaa cttcgactct gcgaaagcga
    15661 ttgctgaatc tatcggtatc cacgttgaga agagctgggg tctgggccgt atcgttaccg
    15721 agatcttcga agaagtggca gaagcacatc tgattcagcc gaccttcatt actgaatatc
    15781 cggcagaagt ttctccgctg gcgcgtcgta acgacgttaa cccggaaatc actgaccgct
    15841 ttgagttctt tatcggtggt cgtgaaatcg gtaacggctt tagcgagctg aatgacgcgg
    15901 aagatcaggc gcagcgcttc ctggatcagg ttgccgcgaa agacgcaggc gacgacgaag
    15961 cgatgttcta cgacgaagat tatgtcaccg cactggaaca tggcttaccg ccgacagccg
    16021 gtctgggaat tggtatcgac cgtatggtaa tgctgttcac caacagccat accatccgcg
    16081 acgttattct gttcccggcg atgcgtccgg taaaataagc attacgttat gctcacaacc
    16141 ccggcaaccg tcggggtttt ttatttaagc tgggtaaatg cagataatcg ttttctggct
    16201 tcgcgatttg tcgcctgcat caccatccac ggactgaacg cccacggcgt agcatcaata
    16261 ccgcgtaaaa ccgctgctaa atcacaccat tgataatcca tcacttcatc atcgttgatc
    16321 tgtaacgcac tggtcgtgcg tgcggcaaat accggacaca cttcattttc cacaatgcca
    16381 ttcggatcgg tggcgcggta gcgaaagtca ggatagatag attcaggagg cgtaatttct
    16441 acgccaagct cataacggca acggcggatc accgcgtctt cgttgctttc tcccagttgt
    16501 gggtgcccac aaaccgagtt agtccacacg ccaggccacg cttttttgct aagggcgcgg
    16561 cgggtaacta ataattgccc tttggcatta aacagccaac tggaaaacgc gagatgtaag
    16621 agggtgtctg ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac tccctgtgca
    16681 ttcaataaaa tgacgtgttc cgtttgcata atatctcaca tgtaattctg atcactcatc
    16741 attatagata atagaagagt gaaatgatta accaagtgag cgtttatcga caaccgcccg
    16801 ttttagcgga tgtcgacagg taaaaaccat ttaaaaaagc agcatcatat atttgaagcg
    16861 actttcaatg gccattgcaa cacaatcaga aatgacagga atagagagag taaaaagcaa
    16921 cagcagactg aatatagcgc gcattaaaag attcatatta ttctccatgc tcatttttct
    16981 tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga
    17041 cggattcctt caccaggtca gcatgatgct ggtagccatt aaagaagacg ttcagcacaa
    17101 cggcgctaag ggttgcgagc ataatgccgc tatgcagcaa cggttgcagt acggccggta
    17161 acttagaaaa gaaatcgtga gagagcgttg gcgtcatgcc aacaccgaga ctgatcgcca
    17221 caatatagag gttgtaacgg ttagtggtgt agttacagcg cgacagaatt cgaatccctg
    17281 tcgccagtac catgccgaac atcactagcc ccgcgccgcc cagcacaaat tgcggaatcg
    17341 aggccaccag caccgccatt ttgggcacca tgccgaacag gatcaaaatg ataccggaag
    17401 caatgcacac ccagcggcta tgaacgcgcg tcacgctaac caggccaacg ttttgagaaa
    17461 atgacgtgtg ggggaagctg ttaaacgttc cgcctatcat tgtccctaca ccatcgacgc
    17521 gcagcccgcg aataatatcg tgtgaagaga gtttacgacc gactatttca cccagtgcca
    17581 ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg
    17641 aaacgggatc gaaaatcggc ataccaaatg acatcggcgt gacaatcgca aaccatgaag
    17701 catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata
    17761 caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca
    17821 agataaaaat taagacggca aaggagatac ctaaataaac gggattacca tattgcggat
    17881 tccctttacc tccggcggcc cagtcaatac ccacctgaat gatgctaagc ccgatagaag
    17941 taatcaccac accggtaacc agtggcggga ataaaggcat caagcgaccg ataagtggcg
    18001 ctaataatgt ggtgataaaa cccgcggcga tagtggcacc gaatatcccc agcaggccga
    18061 tatccgggtt cataccaata gctatcattg gtgttacagc ggcgaaggtc accgacataa
    18121 tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga
    18181 tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcttctttt gataggccga
    18241 gtcggtcacc aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt
    18301 gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc
    18361 ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt
    18421 tcgcccgcca gagtaaaaag caaccgttta tgttcatgaa actgtctaaa cacatgctcg
    18481 tggagtgcca gtttttgcga ggaaggggag ttagagacag gaattgcgga ggcaatcaca
    18541 taatcgtggt taaaccacga aaatgagaag agaacctgac aatggaatta tctgtaattt
    18601 tctcatattg atatttcatt aatcattctg gcatgtcgca ctctcgcatt taatcgtttt
    18661 tatctggata gcattctttt gatcggcaat gttgaaataa tgaaagcggc atagattgag
    18721 gggcaataat ttgaactcag ttgcaaaatt ataattaaaa cggtcatgct acctaatata
    18781 tgcccgttta ttgcatcgcg aaatttgaga agacataact cattaaatga aagggatgca
    18841 aatgaataag tttatcgctg ctgaagctgc ggaatgtata ggctgccatg cttgtgaaat
    18901 tgcctgtgcg gtggcacata atcaagaaaa ctggccgctg agtcacagtg actttcgacc
    18961 gcgtatccac gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc atcactgcaa
    19021 caatgcccct tgcgttacgg cttgtccggt taatgctctg actttccagt ccgatagcgt
    19081 acaactggac gagcaaaaat gtattggttg taaaagatgc gcaatcgctt gcccctttgg
    19141 cgtcgttgag atggtcgata cgattgcaca gaaatgcgac ctttgtaacc agcgcagttc
    19201 cggcacgcaa gcctgtattg acgtctgccc aacacaggcg ttacggctga tggacgataa
    19261 agggttgcag cagataaagg ttgcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc
    19321 agacgctcag ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga
    19381 taaaatttca gcgagtgaac ggaaaaccca ctttggcgaa atctattgcg ggctggatcc
    19441 acaacaagcg acttatgaga gtgaccgctg tgtttattgt gccgaaaaag ctaactgcaa
    19501 ctggcattgt ccgctgcata acgctattcc ggattacatc cgtctggtac aggaaggaaa
    19561 gattattgaa gcggcagaac tttgccacca gaccagttcc ttacccgaaa tctgcggcag
    19621 ggtatgtcca caggaccgtc tttgtgaagg tgcatgtact ttgaaagatc actctggcgc
    19681 agtctctatc ggtaatctgg aacgctacat caccgatact gcgctggcga tgggctggcg
    19741 tcccgatgtc agcaaagttg ttccccgtag cgaaaaagtg gcggtgattg gcgctggacc
    19801 tgcaggatta gggtgtgctg atattctggc gcgcgcggga gttcaggttg atgtctttga
    19861 tcgccatcca gaaattggcg gtatgctgac ttttggcatt cctcctttca aactcgataa
    19921 aacggtatta agccagcggc gagagatatt caccgcaatg ggaatcgatt tccatctcaa
    19981 ctgtgaaatt ggccgcgata tctctttcaa cgaattaacg gcggaatatg atgctgtttt
    20041 cctcggcgtg gggacttacg ggatgatgcg agcagatctg ccgcatgaag atgcgcctgg
    20101 tgtcattcag gcgttaccgt tcctgaccgc ccatacccgc cagctcatgg gattgccgga
    20161 gtctgcagag tatccgctga cggatgtgga aggtaagcga gttgtggtat tgggcggtgg
    20221 cgatacgaca atggattgtt tgcggacttc catccgcctt aatgctgcca gcgtgacctg
    20281 tgcgtatcgt cgtgatgaag tcagtatgcc gggttcgcgc aaagaggtgg tcaatgcccg
    20341 cgaggaaggt gttgagtttc agttcaatgt tcagccgcaa tatatcgctt gtgatgaaga
    20401 tggacgctta acggcggtgg gcctgattcg taccgctatg ggtgagccgg ggccggatgg
    20461 ccgccgtcgt cctcgtccgg ttgcgggttc agagtttgaa ttgcccgccg atgttctcat
    20521 tatggccttt ggtttccagg cacataccat gccgtggttg cagggcagcg gaattaaact
    20581 cgataaatgg ggcctgattc aaaccggcga cgtcgggtat ttacccaccc agacgcatct
    20641 gaaaaaagtc tttgctggtg gtgatgcagt tcatggcgca gatctggttg tcactgcaat
    20701 ggccgcagga aggcaggcgg cgcgcgatat gttaactctg tttgatacga aggcatcgtg
    20761 atgaaatcgt taattatcgt taatccggct gactgtattg gctgccgtac ttgtgaagtg
    20821 gcctgtgtgg tcgctcaccc ttcagaacag gagttgaatg ccgatatctt tttaccccgg
    20881 ctgaaggtac aacggctgga tagcatcagt gcgccggtga tgtgccatca gtgtgaaaac
    20941 gccccttgtg ttggcgcttg tcccgtgggg gcgctgacga tgggcgagca ggtggtgcag
    21001 gcaaattctg cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc gtttggaatg
    21061 atcaccattc agtcattgcc gggggatacc cggcaacaaa tcgtgaaatg cgatctctgt
    21121 gaacagcggg aagaggggcc agcctgcgtt gaatcttgcc cgacacaggc gctgcagttg
    21181 ctgactgaaa gagaactcag gcgagtccgc cagcagcgta ttgttgccag cagtgagaat
    21241 cctctgtgaa agaaatctcc gcgctgaaag gcgcggagaa tctattagat tgcccaacca
    21301 cccgcgtaaa acgcgaccag tgcggcagta atgatcaccg taccaatatt cagcttttgc
    21361 cattcgcgcg caaagacgcg acccaccacc agtgttacaa agcccagcat aataccggta
    21421 acgatattac aagtcagaac gatgaacacg gcacacacca ggccagccat tgcgtcaata
    21481 aaatcgttga aatccagctt cgagacgttg cttaacatca gcaaacccac gtacatcagt
    21541 gcgggagctg tggcataacc ggggatcaga aacgataacg gtgataaaaa cagaatcatc
    21601 aggaacaaca caccaaccac ggttgcggtt aaaccggttt tccccccggc ggcggttcct
    21661 gccgctgatt cgatataaac cgccgcgggc gctgcgccca ccaggccgga gaatattgaa
    21721 cttactgagt cactggtcag ggctttgccg ccgttgatga tctggttgtc tttatccaac
    21781 aaattcgcct gaccggcgac ggcacggatg gtgccagtgg cgtcgaacac tgcggtcatc
    21841 accaatgcca gtacactcgg aagtacagtt ggctggagtg cgcccataat atcgaggctg
    21901 aaaatcagag acttaccatc ttcgccagtc aggcttggca tcgccaccag accgtggtac
    21961 ttcaccgctg ggtcaaagat taagccgatg atcgaaattg caataatcac caacaagatc
    22021 ccgccgggta cgcggcactt ttccagaccg aagatgaccg ccagccccag taagctcatc
    22081 atcaccggga aggaggtaaa ggcaccgagc gccactggca agccttcaat cgggtttttt
    22141 ataaccattc ccacaccgtt agcagcaatc agcagcagaa acaggccgat accgatacct
    22201 gtaccgtgag cgatccccat cggcaaatta cgtaagatcc aggtacgcac accggttacg
    22261 gaaatagcgg tgaagatgac gcccatcaga aagactgcgc ccagtgcgac aggaacgcta
    22321 atttgttgcc cgagtaccag actgaatgcg gtaaacgccg tcaaggaaat agcgcaacca
    22381 atcgccattg gcaaattggc ccataacccc atcagcaacg agccgaagcc cgcgaccaga
    22441 caggtggcaa caaacacagc tgcgggagga aaacctgctt tgcccagcat tcccggaacg
    22501 acgataacgg aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga
    22561 acggtactgc cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt
    22621 gcgtccggtg tttgtaggat gtctccagac ataatgatgt cctcacgagg atgttaaagt
    22681 tttattatta gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac
    22741 ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat
    22801 agcgcagctg ctgtagcgga gtggcagtgg gttccatcac cacgaaatca gcctctttgc
    22861 caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg
    22921 ccaggtaaaa cgcttcatat gccgagaggc gatagccttg taattgcaat accttgtagg
    22981 cttcgttcag cgtttgcagc atgttaaaag ttgttccggc accgatatcc gttcccatgc
    23041 ccactttgac tttcttctgc catgcttttt tcaagttgaa taagccgctg ccgaggtaaa
    23101 ggttggaggt cggacagaaa gcaatgctgg atttggtttc gctgagacga tcccactctt
    23161 tttcttcgag atggacgcag tgagcaaaga cgcagttttt accggtcagg ccgtactgat
    23221 ggtaaacatc aagataacca tcatggtcag gataaagcga tttcacccag gcaatttcat
    23281 ctttgttttc acagagatgg gtatgtaccc atgtatccgg atattcttct ttcaggcgtt
    23341 gcgccatcgc catctgttca ggagatgagg taggggcgaa gcgtggcgta atcgcataca
    23401 acagacgacc atttttgtgc cagcgttcaa tcagttcttt gctttggtga tagctgcttt
    23461 cggcagtgtc gagcagataa tccggtgcgt ttcggtccat catcacttta ccggcaatca
    23521 tacgcatatt gatatgactg gcggcttcaa acagcgcatc aacggattgt ggatgaacag
    23581 tgccaaacac cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg
    23641 acatctcgcg ggcgtactct aaatcctcat aacgacgttc agtagggaag gtgtgtttat
    23701 tcaaccactc cagcaattgt tcaccatagg cccccaccat ttcactttgc ggataatgga
    23761 tatgtgtatc gataaagccc ggtactatca gtttgccgcg atagtcgcgc acgcgaatgg
    23821 tgtcaggaat ttgatgcttt ccgtcttccc attcgccaaa ccattccact tttccctgtt
    23881 taatgagtaa taaaccatcc tcaataaacc gcagcgcaga ggcaatttct tccgggttat
    23941 cgaccgtacg ggtgacatca ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg
    24001 acatcattga ctcctttact gttaatccat ctcttccgct gaggtaatac caggcagaac
    24061 gttttcctgt cggtagccac cagggaggat aatatttaat aaaatcgcag ttaacccgcc
    24121 agcacaaata gggttttcaa ctaatacata aatagaggct ggcaatattt taaaaatttc
    24181 gggatcgtag gagacgccaa gtcctaaacc taaagaagtg gcgacaataa gtgtttctcg
    24241 gcgctttaaa ccgttggtga tgatgatgcg aatccctgcg atggcaatca tggaaaacat
    24301 caacgtcatt gcgcctccca gaactgccga gggaatggtc gtaaaaaagc cgccaatcat
    24361 cggaaataag ccgaggataa ccagtattac cgcgatggtt cgcccgacat aacgtgaagc
    24421 gacgccagtc atctgaataa ccccattatt ttgcgcaaac gtggttaatg gtaatgaacc
    24481 gacagcggag gcgataacag aaaccagacc atctgccagc acgccgcctt tcagtcggga
    24541 ctgatactct tccccctgaa tgggacggcg ggaaaccatt gccgtggcgg tgatatcgcc
    24601 gacagcttcc agcacgctaa gcagataaat cgtgccaacc accaggaact gatggaagct
    24661 aaaactaaag ccgtatttga acggatgcgg gatggtgatt aacggcaaat tgcgcatact
    24721 gctgaaatcc accatgccca ggcataacga tgcgatatag ccgacacata gcccaatggc
    24781 gatcccaccc atgcgtagca acggactgcg gcagcagtta aagccgatca ccacaatcaa
    24841 aaccaataag ccaacgccga gatgttcgta attgccgaac gtaccgctgc ttttggctgc
    24901 aaatcctcca ccaaaatcaa taatgccgac tttaatcagg cttaagccga tcatcagcac
    24961 cacaatgccg ctgacggtag gcgtaataac ccgacgtaaa tagggcagga taaaagaaga
    25021 gccgacaacc agaaatgcgc caacgaagga gacgccgagg agcgacgaca tgattaactc
    25081 ttcgtgaaaa ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg taacaaatga
    25141 aaaattgact gactgaattg aaagtaaacc agaaccgacg atgccgtagc ggtttacttg
    25201 taaccaggta ccaataccag aggcgatcat cgccatagaa acaagatagg cagttgtttc
    25261 agcggaaagc tgtaaggccg cacccacgat taacgcgggg gttaccatcg gaacgaaaat
    25321 tgccaacaga tgggtaatgg cacctacgag agcctgatga aagggagggc gatcctccag
    25381 ttcaaatata aggtcagaac ctgcatggtt tatatcagac atcccttcac tccttttaaa
    25441 gtaaatttga tcatctgaat tagtaaaata aattcccacg aatttggggc gcaggacgcc
    25501 ttgagaacgg cttatctcat tttcgcagtc actatttagt catcgttcaa acagagaaac
    25561 aggccgccga agcggccatt tattacttat attttttcca gcgcggtgag tattttctcc
    25621 ggtgtgaaat gccattcgcg taaccagatg ccgcaggcat cgtgaattgc ggtagcaatc
    25681 gccggagcgg cgccatttac accgatttcc gagatcgatt tcgccccgaa tgggccgact
    25741 ttatcgtcgc tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt
    25801 gcgccgtaac tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata aatgatctct
    25861 tcgctcatac tgtggccgat agcacgcagg gtggcaccgt agatttgtcc cagcgctaac
    25921 tctggattga ccggtgtacc gcagtccagc aaggcgtaga atttatccag gcggatttca
    25981 cccgtacgcg tgttgacggc aacttcagcg aagtttgcgc catacgggaa ggcgaaatca
    26041 ggcgtgatat aactgccagt tcccaccagt gaaccaaagc cggtgccggt ttcgccttta
    26101 tgggctatct ctccgaaact aacttcgcct ttcttgccgc gaacgacgcc cggcgttgct
    26161 agttgaacat ctgccactgg ctcacccaac atttgcgcgc cgtggaacag gattttctcc
    26221 cgcagatttt ccgctgccaa acgcgcagcg ttacccgaga agcaagtacc ggacgaggca
    26281 tatgcgcctt tatcaaacaa cgcatgatcg gtatcaccgg agataacatg cacgtcctgc
    26341 ggggggcagt gcagcacttc tgctgccagt ttcgtcacca cagtatccag gccagtacca
    26401 atatccgcac cgccagaatg aacgataaag gtaccgtctg attccagttt gatcatgcag
    26461 ttagcctgat cgatatctgg gatccctgat ttctgcatga taatggcgac gccgcgaccg
    26521 atgtgccagt caccgttttg tggttttggt gaagaccatt ggatcatctc gcgaccctga
    26581 cgcaggatct cttccagtgc gcagctggcg gcggaaggaa cggaggtcgg cgctttacct
    26641 tcaccgattg caccgagaat tttcagctct tgcccttcgt gtacccggtt acgttcgata
    26701 atttccagtt ggtcgatctg taactgttca gccagttccg ctaatgccat ggtgatagcg
    26761 aagttacctt tcggtgcgcc ataaccctga taagcaccat ttgggcaaat gttgctgtag
    26821 taggtggtga cctggaaatc gacgttatcg catggatata acggcagcga cagcgccggt
    26881 ccgttacacg gtacggtgag tgagtggtta ccgtaagggc cagtgttggc gcggaaatcc
    26941 atcttcactg ccgtcaggcg accatctttt ttcgcgccca gtttgacggt gactttcgct
    27001 acgtgacgag aggtgttagc aataaactct tcttcacggg tgtagcggaa aagtaccgga
    27061 cgcccggtca cgcaagttgc ccaggcgcac acttcttcca gcaggatgtc ctgtttagag
    27121 ccaaaaccgc cgcctacacg ttctttaatg acatgaactt tatgttgttt catgcccacg
    27181 aggcgcgcga cctggcggcg taagtgccat ggtacctggg tggaggcgtg gatcaccaga
    27241 cgatcgccgt ccatacgggt aaagcagata tgtgtttcag tcgggcactg ctgcgcctgc
    27301 gttgagttat aggttcgctc aatgatcaca tcggcatcgg caaagccttt gtccatatcg
    27361 ccaatatgac catgaatact ggcagcgata tttttgcgtg ggcgagaacc gatcgggaag
    27421 ttgatgatca tatgctcgcc gcgctgggct gcatggctgt tatcatcttc cagagtatct
    27481 ggcgcaccag caacatacac caccggttca tcgtgcacga caggcgcatc ttccgccatt
    27541 gcctcgtcaa tcgacattac cggcttaagc acttcatatt caacatcgat gagcttcagt
    27601 gcttcgagcg caatgtcttc actttcggct actaccgcag caacgcgatc gccgacgtga
    27661 cgcattttct tgccgaacat acggcggtca agcggtgatg gttccggtgc gctctgacca
    27721 cccggggtat agtaaatatc cgggcaattc aggtgagtaa taacgtgaac gacgcccggt
    27781 aaggcttcag ctttgctgac atccagatga gtaatcagtg cgtgagcgtg tgggctacgt
    27841 aacattttaa tgacgcaggc gtcagccgtt acgcggtctt caacatagca gggttttgcc
    27901 tgcaccattt tcgcggcatc agttttagga taatgcttgc caatgacttc aaggtcgtca
    27961 cggaaagtcg gagcgatatc aatggtggcc tgcggattat ttttacgtgc aaccgccagt
    28021 tcaatgacct gatagtattg ctgccagcca gcatcgcggc taaacagacc tgagagcgcg
    28081 tcgtcgatct cttcgcgggt aggtgcgtcg atgcgatcga gaagatcggt gataatcaga
    28141 gccgcagctg gatcgttata accagactgc accacgccaa catcaaccat ggcctgttga
    28201 accagactta actcgttcca tttgcccaga gattctgcgg tacgaatatc tgccttctct
    28261 aactgtgcgg cgataagcaa ggacgcgtta acgatattac cgttaaagat aattgcgtca
    28321 gaaccggcaa acccaaaacc atcatcactg ttgcgtacag agtgcattcc catgttaaac
    28381 aacagctttt gcacgttttc gcctggatta acggttagct cctgaagcgc gccatttaaa
    28441 gtaaagtgga tgatcatacg gcttcctccc cagcctgttg gcagtcggca tacaggtcgg
    28501 ctaccagaac tcccgtgata tagcgtttat aggccacgct gccccgcaaa tcttcctgcg
    28561 ggaaaatggc gttggcgaca gcctgttcaa gtgcattgcc ttccagattt tgagtttcaa
    28621 catcatgcag acgcagtgct ttactggcta cgccatccag cgcaattcgc ataccgtcgt
    28681 ggtctgttat tgcaacggct gccgtcacga cggttaaacc tgcctgagaa cggctaattt
    28741 tgcgagtcgc acaggtgcga tacggatctt taatgataat ttcggttaac aggcgatcgc
    28801 atgggcaggc caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa accagttcag
    28861 catccagtgc cagcaggacg ggaagcagca ccgactcttc ctgccgggcg gcgatttcac
    28921 caccaatggt cgactgatta cgaacatggc gtgagtaaac aaaaccgagg gcttcacaca
    28981 gtgctgcagg aataaatcgc gcatcacgca gtggctgcaa gcgagacatt gcgccaattc
    29041 gtagtgcacc gttatcccag tcaatccagt ccagctccag atcctgcaag gaaatggcaa
    29101 tctttttatc ggtacgggtt ggtgtagcgt tgagtttgct gcccccggcg aaccagacgg
    29161 cttcatcctg gtagcggcgc ttcagttcca gcgcctgttc gactgagtcg ggtctgaaaa
    29221 attgttcaat catcgtcttt ccttaatttc ccctcttctc tgaaagaaga ggggagctgt
    29281 tatttatgcc agcgcatcca tccgacgcca catactggca gcggcttttc tggcttgcgc
    29341 ataaatggaa tcgcaatcga agttaaactg acggtcttca tagaccatca caccattgac
    29401 catcacgctg tgaacgctgc ctgagcccat accgaaagcg atatgcccgg cgatattgtc
    29461 tgccagcagc ggcgtcggcg agttgtaatc gcaaatggtt aaatcggctt tgtaaccggc
    29521 ttccagaagc ccaaatttcg cgccaaagtt gcggctcatc agttcgttgc cgttagccag
    29581 tgctttggca aaactgtcag gccacagcgg gccacccgca tcgcgatgtt taaagaaggc
    29641 aaatttcatc tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg
    29701 gatgtcgcta aggtgatggt tgtagccgac atggttgttc atgtttgaac gggcgttatg
    29761 caccaggaac gcatcgcgct gattgagtag ggcgatgtca tctttcgaca agtacagccc
    29821 atgagcgacc agcgttttgc tgtcgatgag atcgaattgc gccagtcgtg ccagcaggtc
    29881 tttgccgtac caatggtgac tgtaggaaac gtcgtaaagg tcttccgcag cgtgaatatg
    29941 caaaccacga cctgtgcttt tcacggcttc acgcagcatc tccagaccgg catccggcac
    30001 ggtaaacggc gcgtgagcgc cgatatgagc ttccaccaga tacggctcgg ttgctgcttt
    30061 cttcgcctca tcaatctgac gggcgaagcg gatgttttct tctacacctt cctgcaactc
    30121 tttgatgccg ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt
    30181 taaaaatgcg ttgcgcaatg tggagagcga cccgtcgata tacgccggag aggcatggtg
    30241 atcgataacc gatgtacatc cactcttaat cgcttccagg gaacaaatca gtccgctgta
    30301 atagagcgac tcttcatcaa gggcgcgatc gagtcgccac cagagatttt tcagcgttga
    30361 gatgaaatcc gggcaggggg cgatgtttgc cataattccg cgggacagcc ccgagtaaaa
    30421 atggttgtgc gagcagacaa ttcccggcat cacaatccga ccatgcatct ctttgtagct
    30481 ggcatcggga tagcgttgcg tcagggcatc gtcgatagcg acaatcacat cattttcgat
    30541 ggcgatatca acgccttcct gcactttcgc cgggtgtaac tgcactgcgg tgacattctt
    30601 cagaatcaac atgattacac ctccacgcgg cccagcagat aatgatgatg ctggtggaca
    30661 tggctaatga tgcggcacat atcgttcagc tccggtggta cgttgttgaa ctgaccttcg
    30721 ctgtcgatgt ttaacaccca gctttggtta ttcagacgta cgcgtacccg gcaatcttcc
    30781 acaaggaagc ctgggttgct gctgttatca aagtcttgcg acaggctgaa gacggtgatt
    30841 ttgtctttgt acggtttacc gttccacggg cagaattggg cgcagttgcc acattcgtta
    30901 cagtaagcgt cgaggtgcag cgtctggaaa cggttctgga agcctgggac cgcaatggat
    30961 acgttggcgc ggttcgggca gacatccaca cacttgctgc aaacgtagtt acattcaagg
    31021 cagcgagcgg cttcctgcgc gacaaacgca tcacgatcgt cactgttcac cagagtgaca
    31081 gagatatcgc ctttacgttg atagatttcc gccggattga cgttattcca gtatttatcg
    31141 ttctggtggg aacggatatt ttcccgactc aggatggcat cggtcgcccg acgcgcggtt
    31201 ccgacagcag cgacaatgga ggatggtccg cgctgtatgt cgccgatcat aaagacgtca
    31261 ctcagacgcg tttcgccatt atggtcgacg tctggccagc cgtttttgtc cagcggcacg
    31321 cccatcgcat tcagggcttc agtatcctgc tgttcaccaa tggcggtgat caggctgtct
    31381 acatgcagtg ttactgtttc gttggtttca accggacggc gacgaccttt ctcatcaggt
    31441 tcgccaagcg acataacgcg caaggttaag gtgccatcag catcgaaacg ttccggatta
    31501 ttcaggaaac ggaactccac gccgtcgtgc aacgcttctt catactcttc gcgccatgct
    31561 ggcatctctt gcagtgaacg acggtaaacg accgttgctt tttctacgcc tggaacgcgt
    31621 aacgccgcac gagcgcagtc cattgccgtg ttacccgccc cgacaacgac cacatgtttg
    31681 cccagcttga gcgctgtgcc cttgttgtat tcacgcagga aggggagtga tttccagaca
    31741 ttttggttgt cgcccgccag tttcacaccg ctatttttat cagtgccggt ggcaatcaga
    31801 acatagtgga agccctgatt ttttaactgt tcaacggtca aatcgggtga gcagccatac
    31861 tcaaatttta cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa ctctgcagga
    31921 atacggaact gaggaatgat atttttcacc acgccgcccg cattggcttc gcgttcaaac
    31981 agcgtaaccg gatggcccgc tctggcaagg aagtaacctg ctgccagacc cgccggacct
    32041 gcaccaatca cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc
    32101 ttatattcat cccaaccttt ttccagcgcg acttttttca gttcgcggat attcagcgca
    32161 ctgtcgtaat ccaggcgggt acagttgtat tggcactggt gatcgcaaat atgaccggta
    32221 atggcgggca gggcgttgcg ttgatagatg agttccagcg cgtcggcata gcggtgttcg
    32281 ccaagcagac ggatgtattc cggaatatct tgcttgatag cgcaggcagt aacacagggg
    32341 gcaacgtagc aatcggtcag cggcaggtct tcagccactt caatacgctc ttctggcttc
    32401 cagtgtttct gggtgtattc catggttaac gcgtctgctg ccagtctgtt cagtcgttcg
    32461 acgtcaacgt ggttaagctc ccaggcatct gagccttcca gctcgcgcat gcaggcactt
    32521 aagcgcagat agccgccagg tttcagcagg tcggttgcca tagtaatagg gcgaatacca
    32581 gtatcaaaaa tatcgcggat agtcagctga ctggcaccac cggaataaga aatgggcagt
    32641 ttgccgtcaa aggcgcgaga gagaactgct gcaacgttga tggagagcgg gaacagcgca
    32701 cggcctgaca tatacatctc ttcaccgggc aatgcgcctt tattgttgat ggtgccgaga
    32761 gtgttagtca gttttacgcc aaagccgagt gatttttctt ttgccagtgc catcaggcgt
    32821 tccagcattt ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc ttcttttaag
    32881 ccgatgtaac cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta ccccagtaag
    32941 gtcgggttga gtttcacaaa ggtgttgagc cctttttctt ccagcatgta gcggcaaatg
    33001 gcttcgattt catgcggagg acagccgtgc atggtggaga gggtaacccc ttgtaccatg
    33061 ctggtgggga tgcgagcggg taaggcttgc aagctttcgc gtttttcctg caatccgtgg
    33121 cgggctaaaa atgcctcatt ctggagtagc ttattaagtg catcgcggta ttgagcgaat
    33181 ttcggatggt cagatgcgtc catcatattg tcgatgaact gctgcatcgg cggttgctta
    33241 ataccttcga ggttgtaacc gacgctcata ttaaagatga acgatttacc ggaatcagaa
    33301 ggctggaaca tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag
    33361 gctttaagca gggtaaattc ggtagaccat tcggtgttaa agcactcgtc ttcggcatcg
    33421 atacagggct tttccagctc caggcggtca agaatttgga cggtttttag ttcgatgaag
    33481 cgtccgccag tcagccagga ggtgacgatg ttttgcgcaa gttgcgtgtg cgggccagcg
    33541 gcagggccga cgggagtggc acaggtttca ccgaagacgc taacagtttt accttttacg
    33601 ggtgagtaaa actgttgctc gggaatacca aagattgagc gttgttgttg gtattcatca
    33661 aatatgcgcg tcaaaagttc ctcaaacgga atgggacgca taatatcccc cataaccctc
    33721 tccttagctt atacagatat cattgagtga ccggtgttga gaaatccggt ggtttagggg
    33781 gatagagcaa caatcgcacc actcttgcca ggtttggcgg gaaatataaa aaaatgtgag
    33841 gcggctcgaa acttaatggc tttttgtgaa tgaactcaca ggtgccaaat gagaattaaa
    33901 atcactcctg aaaattatta ttgccagccc atttatcact ttttctcttt tctcaaaatc
    33961 agtgattcct tatcattatg ataaagagtg ctggagggat tagatatttg aacgcaatcg
    34021 tttaactacg ttttctaaat ttcagtatac cttttcttcg ctgtaataaa gtcgtccgga
    34081 gtatcaatat ctaaaattat ttctgcattt tctatttcga cggaataatg ttctcccata
    34141 aggagtgcct gacgcatatt ggtgacattg ggtcgctgga ttgcctgcat caggcatggt
    34201 tttgacacta aaatcggatg gccggggatg ccattatgga gtggcagtat tgcgccatca
    34261 tttcgtaacg accagatttt tctaaaaata tcgatggtga gggttggtat atctccgtgg
    34321 gtgagaaaac aatgttctgt ttgcaccgcg ggtactgcgg cctttactga tgtcagtaaa
    34381 ccctgcgcat aatctgggtt gtggataata gtgatattgc tctggttcgc ataacgttcg
    34441 tgcagttcat taccacgata gccggtgact aaaataattc ggctacaaaa ctgcaacgca
    34501 tttttgatac ttgtatcaag aattgttccc tgttgccagg gtaacatcat tttccattgc
    34561 cccattcttg atgataatcc tgcggcggta attatacagt cgatggctga catagtgatt
    34621 ccattattta caggagctaa taacgcctga taattgcgtt acttcctgat ggattacgtt
    34681 gagggtaatt tatcccagag aggtccataa agacgcgtga aaagtataat tgacccatcg
    34741 gcattattca ttgatttagg cgcgcagaaa cgtcccactg tgatttctat tgttggtgct
    34801 ggggggaaaa ccagcttgct tttttggctg gcagaactgt tccaggcgag cggcaggcgt
    34861 gtattaatca ctacgacgac acatatgttt atgccaacat ctcactggct cgtggttttc
    34921 tgtcgtgatc ccgccatgct tcctcatgcg tcttttatat cccccatttc attttgtttt
    34981 cacagctgga aagcgaacca gggaaaagtg cagggattta cgccagaagc gattgatgca
    35041 ctggtacaac gaccagagtg tgacgtaatt ctcattgagg cagatggctc gcgtggaatg
    35101 ccgttaaaag cgcctgatga gcacgaacct tgcataccta aaagcagttg ctgcgtgatt
    35161 gctgtgatgg gaggacatat tttgggtgag aaagtgagca cagaaaatgt ccatcgctgg
    35221 tcgcagtttg ctgatattac ggggttaaca cctgatgcac ccttgcaact gagcgatctc
    35281 gttgcgctgg ttcgccaccc tcagggagcg tttaaaaacg taccacaagg ttgtcggcga
    35341 gtctggttca ttaaccgttt ttctcaatgt gagaatgcga ttgcgcaaag cgagctcctt
    35401 caaccgctgc aacaacacaa cgtagaggca atctggctgg gcgatataca agaacatcct
    35461 gcaatcgcgc gcagatttgt gaattagcgg cccgggtatt ccgggttact gaaaggatat
    35521 ccattgaggt tggtatgaat attttcacag aggctgcaaa actcgaagag caaaattgtc
    35581 cgtttgcgat ggcgcaaatt gttgatagcc gaggctcaac cccccgccat tctgcacaaa
    35641 tgttagtgcg cgccgatggt tctatcgtcg gtacaattgg tggcggaatg gttgagcgga
    35701 aggtgattga agagtcgctt caggcattgc aggaacgtaa gccgcgatta ttccatggac
    35761 gtatggctcg taacggtgcg gatgctgtcg ggtcagattg tggaggtgca atgtcagtat
    35821 ttatcagcgt ccatggtatg cgtccacgtc tggtgttgat tggtgcgggg catgtcaacc
    35881 gggcgatagc ccagagtgcg gcgctattag gatttgatat cgccgttgct gatatttatc
    35941 gcgaaagcct caatcctgaa ctattcccac catcaaccac gcttctccat gctgagtcgt
    36001 ttggtgcggc agtggaagca ctggatattc gccctgataa ttttgtcctg attgccacga
    36061 ataatcagga tcgtgaagcc ctcgacaaac tcattgaaca acccattgca tggctggggt
    36121 tgctggcaag tcgccgcaag gttcagcttt tcctgcgcca attgcgtgag aaaggcgtgg
    36181 ctgaagaaca tattgcccgt ttacatgcgc ccgttggtta caacataggt gcggaaacgc
    36241 cgcaggagat cgccatcagc gtgctggcag aaatattaca ggtgaaaaat aacgcgccgg
    36301 gtgggctgat gatgaagcct tctcatcctt ccggacacca gctggtggtg attcgcggtg
    36361 cgggggatat cgccagtggt gtagcgctac gtctgtatca tgcgggtttt aaagtgatca
    36421 tgttggaagt ggaaaaaccg acagtgattc gttgtaccgt ggcgtttgcc caggccgtgt
    36481 tcgatggcga aatgacggtc gaaggcgtca ccgcccgcct ggcaaccagc tctgcggaag
    36541 cgatgaaact taccgaacgc ggattcatcc ctgtgatggt agatcccacc tgttcattgc
    36601 ttgatgaact gaaaccgctt tgcgtggtgg acgctattct ggcgaaacag aatttgggta
    36661 cgcgggcaga tatggcacca gtaacaatcg cgcttgggcc gggctttact gcagggaagg
    36721 attgtcatgc ggtaattgaa acaaatcgcg ggcactggct cggtcaggtg atttactcag
    36781 gttgtgcgca ggagaatacc ggtgttcccg gcaatattat ggggcatacc acccgacgag
    36841 tgatccgcgc tcctgctgca ggcattatgc gatccaacgt gaaattaggc gatctggtga
    36901 aagagggcga tgtcattgcc tggattggtg agcatgaaat taaagcaccg ttgacgggga
    36961 tggtgcgtgg cttgttgaac gacgggctgg ccgtggtcgg tggttttaaa attggtgata
    37021 tcgatcctcg tggtgaaacg gctgatttca ccagcgtttc tgataaagcc cgggcgattg
    37081 gcggcggcgt acttgaggcg ttaatgatgt tgatgcatca gggcgttaaa gcgacaaaag
    37141 aagtgctgga agtggcttaa atgaaaatgg ccggatggta ttacccatcc ggcctttaaa
    37201 ttactgacaa atcaccgttc cggttttgcc ttcaatccct tctttcgctt tgctcaatac
    37261 ggtaatcagt gcttcacgac ctgcgcgaga gcgagcaaac gaggcggcag cttccacttt
    37321 aggcagcata gaacctttcg caaaatgacc ttcttcgata aagcgttcag catcacttaa
    37381 cgacaggcga tcgagccact gttcattctc ttttccgaag ttaatggcca ctttttctac
    37441 cgcagtcaga atgatcagca tatcggcatc aatcatttct gctaaacggg cgctggccca
    37501 gtctttatcg ataaccgcgc tggcaccacg cagatggtta ccttcacgaa taacagggat
    37561 accaccaccg ccaacggtga tcaccacctg tccggcatct accagagctt taaccgtttc
    37621 tttttcaatg atatcaaccg gttttggcga agcaacgaca cgacgatagc cgcgacccgc
    37681 atcttctttc agagtgtaac cctgttttgt cagtagctct gcttcttgct cggtaaagaa
    37741 cgaaccgatc ggcttggtcg ggttgaggaa tgccggatcg ttagcatcga cttcgacctg
    37801 ggtaaccagc gttgctacag gtttattaat gccacgagaa agcagttctt ctcttaaggc
    37861 gttttgtaga tcgtaaccaa tataaccctg gcttaaggca acacagacag acatcggcag
    37921 cattggcgag tgcgcttcag tttttgcggc agcttcaaag gcctggttaa tcatgcccac
    37981 ctgtggcccg ttgccatgag tgacgatgac ctcatgtccc tgggcaatta aatcaacaat
    38041 tgcctgagaa gtaattttta ccgctttcat ttgtccggcc aggtcatcgc ccagcgcatt
    38101 cccgcccagg gcgagaacaa ttttcttact catttttcct cactcatcat tcttgattgt
    38161 gtatagatcc ctggcagtgg ttagagcacg ggagggacaa acggtttgcg tcgcaggaaa
    38221 cgacctcgcc cggctttgcc tgtaaaggtg ccgtcgcaga aaatcgtttc accacgggat
    38281 aaggttctga caatcgcgcc ctgacaggta aacccctccc atggcgagta gtcagcgttg
    38341 tcgtggagat ggcgatgctg aatttgttgg ctctgacgtg ggtcgataat caccacgtca
    38401 ccatcggaac cgggcgctaa tatccctttt tgcggccaca ggccaaacag cctggcgggc
    38461 attgcactgg ttaactcaac aaaacgctcc ggtgagatac gtcccgtcat cacgccactg
    38521 gagaacagca gttgcatgcg gttctccaca ccgggtaagc catttgggca gcggctgaaa
    38581 tcgcctttag aaatttgcag gcgctgggcc atcgagaagg tgcagtgatc ggttgccacc
    38641 acgtcaatcg caccatcgct gatgccacac cacagtttgt cctgctcgcg tacgttacgc
    38701 agcggtgggc taagaatgaa cttcatgcca tcttctgtat cgtaactgcg ttcgtccaac
    38761 aggagatatt gtgggcaggt ttcaacccag actggctggt gattcgcacg ggcaagacgc
    38821 agataatcca gacctaagcc gttagacagg tgcacgatat agagcggggc gttaccggca
    38881 atttgtgcca ggttaatcat gcgggcgatg gcttccgctt cgcattccag aggtcgactc
    38941 aaggcatgat aacgcggcgc ggttaaccct gcggcgataa actccgcccg cttgctggcg
    39001 atagctgcgt cattttccgg gtgtacggtg gtcagcgcgc cggactcatg caagcggcgt
    39061 aatgcctgca acacttcatc atcgttgagt ttatattgat aggttaaata gagtttaaaa
    39121 ctgctcagtc cttcctcaac catcatcgga atttcgtcga ggatagcgtg attaatatgt
    39181 tgaatgacac cgtgaaagct gtagtcgatg actgctttgt gggcggcata accgcgataa
    39241 acttccagtt gatggcgtaa ccggcagcca tttgggccaa atcccatatg gtcaataatg
    39301 gttgttgtac cgccacacgc agcagcgcgg gtaccggtaa aaaaatcatc acaactgcgc
    39361 gcgatgccga catcaatatt gaaatgcgta tggacatcca cgccgccagg gaaaacgtaa
    39421 cagccagagg catcaatttc ttcgcaaggg agttgcggcg aaatatcggt acccaactgg
    39481 cgaacaatcc cgctttcaat cagcaaatcc tgcttcgcct gtccatctgc attagcaaca
    39541 atgccgtttt ttatcaatac gcgcatagca aactccaaaa accctccggc gaaccggagg
    39601 ggaataagtg aagagtaatt attcggttgc cagccagctt aatgggattg cagcgtacat
    39661 agcggcacag gttaccaggt gagatttcca ggttttttcg tttggcgcgt gcgcttccgg
    39721 ttctttacca gggccaaaac cgatgaccgg aatgccgtgg cgacccatga tggatacgcc
    39781 attagtcgag aaggtccact tatcaacgac aggcgcttta ccaaacagcc cttcataagc
    39841 attcaccagt gctttaacgg tgaagtgatc ttcttccact ttccaggtcg ggaagtagca
    39901 ttcggttggg taaaccaggc cagtccagga tgggcggtcg tagttgtaca tagaaacaac
    39961 cgcgttagct ttctgtactg caggcagggc gcggatctcg tccagcgcgc cttcccaggt
    40021 ttcgccccag gtcagacgac ggtcgattga aactgcgcag ctgtctgcga cagcgcaacg
    40081 gcttggggag gtgaagaaaa tttcagaaac ggtgagggtg cctttgccga ggaattcgtc
    40141 ataacccaga cgttgggaga gttcttgtaa ttcgccaaga attggaccca ttttgaaaat
    40201 ggcgttatca ccacgttctg gtgccgaacc gtggcagcta acaccctgaa catcaatgcg
    40261 aatttccata cgaccgcgtt gaccacggta tacctggcag tcggttggtt cggtactgac
    40321 cacaaattcc gggcgaatgc cggattgttc aataatgtat tgccagcaca gaccgtcgca
    40381 gtcttcttcc tgcacagtac cggtgaccag cagggtatat tcatcttcca gaccgagatc
    40441 tttaatgatt tttccggcat ataccataga cgccatgccg ccttcctggt cggaagtacc
    40501 acgaccaccg atcagctcgt cggtttccat gccttcatac ggatcgaagt cccagttttt
    40561 gatgttgcca atgccgactg tatcgatatg agcgtccatt gccaccagac gcgggccgtg
    40621 gccgatataa ccgagaacat tgcccattgg gtcgatttca actttatcga agccgacttt
    40681 ttccatctct tctttaatac gatgtactac gcgtttctcg tcgcagcttt cactgggaat
    40741 agcaaccata tcgcgcagga agcgagtcat gtctgcctgg taatcttttg ctttttcaag
    40801 aatcagtttg aatggaatat tcttagccat tatttttctc cagatttttt actccccgtt
    40861 ttgcagggag cagttccaat agggtgatta aggtgctaca gcgtgtttgc cttcccagac
    40921 aacttcgcgg tagtgcttca cgtcggtgtc gccttcagtg ctgataacca gcaccacggc
    40981 atctttgttc agcgccagtt tttccatcag gctttgacgt tgcgggtgat aatgaaccgc
    41041 tgcgagaacg cccaaaccga cagcgccgga ttcaccggag atgatgcgcg ggtcgttgcc
    41101 atacggatta cccagcacgc gcatacctaa tgcggcaacg ctgtcctggc aggagatgaa
    41161 ttgggtggcg cagttacgta ggatttccca gcccagtggg ttaggttcgc cgcaggccag
    41221 gcccgccatg atggtggcca tatcaccgcc aacgttgacg atgtcgcctt tgacgccgga
    41281 gcgataaata cagtcagctt tgtcaggttc aacaataatg ctgtgcagat tttgcgggct
    41341 atagacgtcg accagataac ccagcacacc accggccatt gcaccgacac cggcttgcag
    41401 cagaacgtgt gtcggggtga cgcccatttc acgcatttgc tcgacggctt catccgccag
    41461 tgttgcgtag ccttgcatga tccaggttgg gattttggtg taaccttccc atgccgtgtc
    41521 ctgtaccact tcccagccgt gctgctgcgc gtgttgcatg gtcaggcgaa cggtatcgtc
    41581 atagttcata tccgtgacga tgcactcggc accgaggttt agaatggcgt caacgcgttc
    41641 ctgagcagaa cctttcggca tgtaaatcac cgcattctgt ccgagttgct gtgctgccca
    41701 tgccacaccg cgcccgtggt tgccgtcggt ggtagtcgcg aaagtcattt tttcgccgat
    41761 ggcatttttc aggtgctcaa atgacagcgt ttcgatatca agatgatatt tttcacacaa
    41821 taattgagcg atggcgtacg caccgccgag cattttgaag gcgttcagac cgaatcgttt
    41881 tgattcgtct ttgacgagaa ttttctttac accaaacagg tttgcgagat cgtccagcgc
    41941 acaaagcggt gttgggtgat aaccagcaat tttctggtgg aactggcgtg ccagttttgc
    42001 ctggctttgc gaaaaaagcg gtgatgtttc gccgttgaaa aatttgttat cggcgatatc
    42061 aatcttcaat gagaaaacgg acatatccta tccttttggc ctctggaaat agagggccga
    42121 ccagctgatc ggcccgaggg gttatttcac gcgttcttgc gcttctttca gcagttgctc
    42181 gagcagtgca cctggtttgg cgtatttacg ggacaggatc atcgcggcga tgatataagg
    42241 cttccagctg gcttctttgt aggtagcgat acggtatttt tcgaatacgc cttcagtcac
    42301 ttcaccttct ttgcaggata cgccgctgat atcagctggc aggcagtgca tgtacagggc
    42361 ttcgccatca cgggtcagtt ccatcatctc ttcagtacaa tgccagtctt tgtgttgcgc
    42421 gttctgtgcc agacactgtt tttccagtgc ttttaagcct tcgtgatcgt tcgcacgcag
    42481 caattcagta cgctcttcca tcactttgta aggtgcccat gacttcggat aaacgatgtc
    42541 tgcgtctttg aaggcttctt ccatgctggt gacctgacgg aagctaccac cggaggcttt
    42601 agcattgttt ttagcaactt caacgacatc cgggatcagg tcgtagcctt ccggatgggc
    42661 cagggtgaca tccataccga agcgggtcat cagacctatg atgccttgcg gtacagagag
    42721 cggtttgcca tagcttgggg agtaggccca ggtcatggcg atttttttgc ctttcaggtt
    42781 ttccagcgaa ccaaagtgtt cacgcaacca tgccaggtca gccatagact gagtcgggtg
    42841 gtcaatatcg cattgcaggt tcactaaagc cggacgctgc ggcagcacac cctgcttgta
    42901 gccgtcatca agtgcagcgc caacttcacg catataagcg ttgcctgcgc ccagatacat
    42961 atcgtcgcga ataccgatag cgtcagcgca gaaggagatc atattggcgg tttcacgcac
    43021 ggtttcgccg tgagcgattt gtgatttacc ttcgtcaaga tcttgttgtg caaggccgag
    43081 caggttaagc gcggaagcat aagagaagcg ggtacgggtg gagttgtcgc ggaatacgga
    43141 aatacctaat ccactattaa agactttggt tgagatgttt tcagcacgca gtgcttttaa
    43201 cgcggcggca acgtccagta cttgtttcag ttcgtctggc gtctgttccc acgttaacaa
    43261 aaaatctttt tcatgcaggt gagaggtcag cgaattgata tccttaatca gctcattaac
    43321 agttttcatc ataaagtccc gatagtggaa tgacaggcaa agtggtttat ttcactgtga
    43381 cgtgccaaag tgattggtgt tttcacagct atacctggga ctaacaataa gtgtgccaga
    43441 attatccacg cggctgcgaa aggctaattt gacggataat gtgtggggtt gatcacatat
    43501 tgatgccagt ttccggttgt ttttaaaata cga
//
LOCUS       DARNVM010000017        42485 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.017, whole genome shotgun sequence.
ACCESSION   DARNVM010000017 DARNVM010000000
VERSION     DARNVM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.017"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            112..198
                     /gene="leuL"
                     /locus_tag="SI621_000922"
     CDS             112..198
                     /gene="leuL"
                     /locus_tag="SI621_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HEI4063666.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            291..1862
                     /gene="leuA"
                     /locus_tag="SI621_000923"
     CDS             291..1862
                     /gene="leuA"
                     /locus_tag="SI621_000923"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEI4063667.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            1862..2953
                     /gene="leuB"
                     /locus_tag="SI621_000924"
     CDS             1862..2953
                     /gene="leuB"
                     /locus_tag="SI621_000924"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEI4063668.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            2956..4356
                     /gene="leuC"
                     /locus_tag="SI621_000925"
     CDS             2956..4356
                     /gene="leuC"
                     /locus_tag="SI621_000925"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEI4063669.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDNAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4367..4972
                     /gene="leuD"
                     /locus_tag="SI621_000926"
     CDS             4367..4972
                     /gene="leuD"
                     /locus_tag="SI621_000926"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEI4063670.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(5080..5211)
                     /gene="sgrT"
                     /locus_tag="SI621_000927"
     CDS             complement(5080..5211)
                     /gene="sgrT"
                     /locus_tag="SI621_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HEI4063671.1"
                     /translation="MRQFYQYYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5300..6958
                     /gene="sgrR"
                     /locus_tag="SI621_000928"
     CDS             5300..6958
                     /gene="sgrR"
                     /locus_tag="SI621_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HEI4063672.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     EIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETISNFASHPIGTGPYGVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLESLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7455..8291)
                     /locus_tag="SI621_000929"
     CDS             complement(7455..8291)
                     /locus_tag="SI621_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4063673.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMVNLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNCDDYSKYFTGAVDEKARDRFTHRLKK
                     FNELKEHAKDPEMMQARGSISPDNMLCVSFYIGNIEIYTQKLQLGISPSTGGIDLSNA
                     YLSGISLNGACLRKADLSNAEMNKISLCSSNLLGADLHGAKMNNAKVISSDLSDTNLS
                     DTDMSDTDLDDTLLRNAKMDNTILNNAYMEDTNVRGINLSKADLSGQDSAKLRSRGAI
                     ID"
     regulatory      8532..8631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8662..9645
                     /gene="thiB"
                     /locus_tag="SI621_000930"
     CDS             8662..9645
                     /gene="thiB"
                     /locus_tag="SI621_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HEI4063674.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9621..11231
                     /gene="thiP"
                     /locus_tag="SI621_000931"
     CDS             9621..11231
                     /gene="thiP"
                     /locus_tag="SI621_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HEI4063675.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPTRAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11215..11913
                     /gene="thiQ"
                     /locus_tag="SI621_000932"
     CDS             11215..11913
                     /gene="thiQ"
                     /locus_tag="SI621_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HEI4063676.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTSTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKARASALLGITG"
     gene            complement(12027..12791)
                     /locus_tag="SI621_000933"
     CDS             complement(12027..12791)
                     /locus_tag="SI621_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4063677.1"
                     /translation="MQALLEHFITQSTVYSLIAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(12877..13755)
                     /gene="araC"
                     /locus_tag="SI621_000934"
     CDS             complement(12877..13755)
                     /gene="araC"
                     /locus_tag="SI621_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HEI4063678.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14094..15794
                     /gene="araB"
                     /locus_tag="SI621_000935"
     CDS             14094..15794
                     /gene="araB"
                     /locus_tag="SI621_000935"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEI4063679.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15805..17307
                     /gene="araA"
                     /locus_tag="SI621_000936"
     CDS             15805..17307
                     /gene="araA"
                     /locus_tag="SI621_000936"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEI4063680.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQVHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17476..18171
                     /gene="araD"
                     /locus_tag="SI621_000937"
     CDS             17476..18171
                     /gene="araD"
                     /locus_tag="SI621_000937"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEI4063681.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18246..20597
                     /gene="polB"
                     /locus_tag="SI621_000938"
     CDS             18246..20597
                     /gene="polB"
                     /locus_tag="SI621_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HEI4063682.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     IYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFKLEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RTLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20762..23668
                     /gene="rapA"
                     /locus_tag="SI621_000939"
     CDS             20762..23668
                     /gene="rapA"
                     /locus_tag="SI621_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEI4063683.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23680..24339
                     /gene="rluA"
                     /locus_tag="SI621_000940"
     CDS             23680..24339
                     /gene="rluA"
                     /locus_tag="SI621_000940"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HEI4063684.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24552..24730
                     /locus_tag="SI621_000941"
                     /pseudo
     CDS             24552..24730
                     /locus_tag="SI621_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24747..25508)
                     /locus_tag="SI621_000942"
     CDS             complement(24747..25508)
                     /locus_tag="SI621_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4063685.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGKMRWVAEIYGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRDCYFGKKTIIDSNENLYMKPGWNANLDGATLDGAILDGATVDGATRLYAEVI
                     IINKITPKKIVTEGVATKQSTAEKLLPTQLLNDPE"
     gene            complement(25803..26618)
                     /gene="djlA"
                     /locus_tag="SI621_000943"
     CDS             complement(25803..26618)
                     /gene="djlA"
                     /locus_tag="SI621_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEI4063686.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26873..29227
                     /gene="lptD"
                     /locus_tag="SI621_000944"
     CDS             26873..29227
                     /gene="lptD"
                     /locus_tag="SI621_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEI4063687.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29280..30566
                     /gene="surA"
                     /locus_tag="SI621_000945"
     CDS             29280..30566
                     /gene="surA"
                     /locus_tag="SI621_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HEI4063688.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30566..31555
                     /gene="pdxA"
                     /locus_tag="SI621_000946"
     CDS             30566..31555
                     /gene="pdxA"
                     /locus_tag="SI621_000946"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEI4063689.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     NRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            31552..32373
                     /gene="rsmA"
                     /locus_tag="SI621_000947"
     CDS             31552..32373
                     /gene="rsmA"
                     /locus_tag="SI621_000947"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEI4063690.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32376..32753
                     /gene="apaG"
                     /locus_tag="SI621_000948"
     CDS             32376..32753
                     /gene="apaG"
                     /locus_tag="SI621_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEI4063691.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32760..33608
                     /gene="apaH"
                     /locus_tag="SI621_000949"
     CDS             32760..33608
                     /gene="apaH"
                     /locus_tag="SI621_000949"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HEI4063692.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(33605..33721)
                     /locus_tag="SI621_000950"
     CDS             complement(33605..33721)
                     /locus_tag="SI621_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEI4063693.1"
                     /translation="MTHETATAPVNALGTKFCDASAHRTLIKGALDFMLDGI"
     gene            complement(33718..33906)
                     /locus_tag="SI621_000951"
     CDS             complement(33718..33906)
                     /locus_tag="SI621_000951"
                     /inference="COORDINATES: protein motif:HMM:NF013965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEI4063694.1"
                     /translation="MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFI
                     RIRPPERLNTILLLVDGS"
     gene            complement(33909..34142)
                     /locus_tag="SI621_000952"
     CDS             complement(33909..34142)
                     /locus_tag="SI621_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEI4063695.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(34228..34707)
                     /gene="folA"
                     /locus_tag="SI621_000953"
     CDS             complement(34228..34707)
                     /gene="folA"
                     /locus_tag="SI621_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEI4063696.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34899..36761)
                     /gene="kefC"
                     /locus_tag="SI621_000954"
     CDS             complement(34899..36761)
                     /gene="kefC"
                     /locus_tag="SI621_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEI4063697.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36754..37284)
                     /gene="kefF"
                     /locus_tag="SI621_000955"
     CDS             complement(36754..37284)
                     /gene="kefF"
                     /locus_tag="SI621_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HEI4063698.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37392..38723)
                     /locus_tag="SI621_000956"
     CDS             complement(37392..38723)
                     /locus_tag="SI621_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063699.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38782..39069)
                     /gene="fixX"
                     /locus_tag="SI621_000957"
     CDS             complement(38782..39069)
                     /gene="fixX"
                     /locus_tag="SI621_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HEI4063700.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIIVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(39066..40352)
                     /locus_tag="SI621_000958"
     CDS             complement(39066..40352)
                     /locus_tag="SI621_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEI4063701.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDESSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40403..41344)
                     /locus_tag="SI621_000959"
     CDS             complement(40403..41344)
                     /locus_tag="SI621_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEI4063702.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVCVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41359..42129)
                     /locus_tag="SI621_000960"
     CDS             complement(41359..42129)
                     /locus_tag="SI621_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HEI4063703.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEGQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAT
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVEA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIVAFAENLRKVI"
BASE COUNT        10215 a        11516 c        11032 g         9722 t
ORIGIN      
        1 tgacggatgc agaactcatg ctggcgggac gtttttatta cgtcagggtt gacatccgtt
       61 tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac
      121 atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg
      181 agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg
      241 tttttttatg cccgaagcga ggcgctcaaa aagagacaag gacccaaacc atgagccagc
      301 aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga
      361 gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg
      421 aagtcgggtt ccccgtctct tcgccgggtg attttgaatc agtgcaaacc atcgctcgcc
      481 aggttaaaaa cagccgcgta tgcgcgttag ctcgctgcgt ggaaaaggat atcgacgtgg
      541 cggccgaatc tctgaaagtc gccgaagcct tccggattca tacctttatt gccacttcgc
      601 caatgcacat tgccaccaag ctgcgtagca cgctggacga ggtgatcgaa cgcgctatct
      661 atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg
      721 gacgcacccc aattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca
      781 ccaccatcaa catcccggac accgtgggct acaccatgcc atttgagttc gccggaatca
      841 tcagcgggct gtatgaacgc gtacccaaca tcgacaaagc cattatttcc gtacataccc
      901 acgacgattt gggcctggcg gtcggaaact cactggcggc ggtacatgcc ggtgcacgcc
      961 aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcc ctggaagaag
     1021 tgatcatggc gatcaaagtt cgtaaggata ttctcaacgt tcataccgcc attaatcacc
     1081 aggaaatatg gcgcaccagc caattagtta gccagatttg taatatgccg atcccggcaa
     1141 acaaagccat tgttggcagc ggcgcattcg cacactcctc cggtattcat caggatggtg
     1201 tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt ttgaaccaaa
     1261 tccagctgaa tctgacctct cgttcgggac gtgcggcagt aaaacaccgc atggatgaga
     1321 tggggtataa agaaagtgaa tataatttag acaatttgta cgatgccttc ctgaagctgg
     1381 cggacaaaaa aggtcaggtg tttgattacg atctggaggc actggccttc atcggtaagc
     1441 agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg
     1501 atattgccac tgccgccgtc aaactggcct gcggcgaaga agtcaaagca gaagccgcca
     1561 acggtaacgg tccggtcgat gccgtctatc aggcgataaa ccgcatcact gactataacg
     1621 tcgaactggt gaagtacagc ctgaccgcca aaggccacgg taaagatgcg ctgggtcagg
     1681 tggatattgt cgccaactac aatggtcgcc gcttccacgg cgtcggcctg gccaccgata
     1741 ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgcgccgcag
     1801 aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt
     1861 gatgtcgaag aattaccata ttgccgtttt gcccggagat gggatcgggc cggaagtgat
     1921 gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc gcatcaccac
     1981 cagccattac gatgtaggcg gcgcagccat tgataaccac gggcagccgc tgccgcctgc
     2041 gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gtggtccgaa
     2101 gtgggaacat ttgccgccag accagcaacc agaacgcggc gcactgttgc ctttgcgtaa
     2161 acacttcaaa ttattcagca atctgcgtcc ggcaaaactg tatcaggggc tggaagcatt
     2221 ctgtccgctg cgtgctgaca ttgccgctaa cggcttcgac atcctgtgtg tgcgcgaact
     2281 gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa
     2341 agcgtttgat accgaggtgt atcaccgttt tgagattgaa cgtatcgccc gcatcgcgtt
     2401 tgaatctgct cgtaaacgtc gccataaagt gacgtctatc gacaaagcca acgtgctgca
     2461 atcctctatt ttatggcggg agatcgttaa cgagatcgcc acggaatacc cggatgtcga
     2521 actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt
     2581 tgacgtcctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat
     2641 cactggctca atggggatgt tgccttccgc cagcctgaac gaacaaggtt ttggtctgta
     2701 tgaaccggca ggcggctccg caccagatat cgcaggcaaa aacatcgcca acccgattgc
     2761 acaaatcctg tcgctggcac tgctgctgcg ttacagcctg gatgccgatg atgcggcttc
     2821 cgccattgaa cgcgccatta accgcgcatt agaagaaggc attcgcaccg gagatttagc
     2881 ccgtggcgct gccgccgtta gtaccaatga aatgggcgat atcattgccc gctatgtggt
     2941 agaaggggtg taatcatggc taaaacgtta tacgaaaaat tgttcgacgc tcacgtagtg
     3001 tacgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg
     3061 acctcaccgc aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggg
     3121 aaaaccttcg ccactatgga tcacaacgtc tctactcaga ccaaagatat taatgcctgc
     3181 ggtgaaatgg cgcgcatcca gatgcaggag ctgatcaaaa actgcaaaga atttggcgtc
     3241 gagctgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag
     3301 ggcgtcacat tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc
     3361 gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaacg
     3421 caaaccctga aacagggtcg tgcgaagacc atgaaaattg aagtccaggg caaagccgcg
     3481 ccgggcatta ccgcgaaaga tatcgtgctg gcaattatcg gtaaaactgg cagcgcaggc
     3541 ggcaccgggc atgtggtgga gttttgcggc gaagcaatcc gtgatttaag catggaaggt
     3601 cgtatgaccc tgtgcaatat ggcaatcgaa atgggcgcga aagccggtct ggttgcaccg
     3661 gacgaaacta cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc
     3721 gacaacgccg ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc
     3781 gttgtgactt tgcaggcaga agaaatttca ccgcaggtca cctggggcac caatccaggt
     3841 caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa
     3901 cgcgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggtat tccgctgaca
     3961 gaagtggcta tcgacaaagt gtttatcggt tcctgcacta actcgcgtat tgaagattta
     4021 cgcgcggcgg ctgaaatcgc caaagggcgg aaagtcgcgc caggcgtgca ggcactggtg
     4081 gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga caaaatcttt
     4141 attgaagccg gttttgaatg gcgcttgcca ggttgctcaa tgtgtctggc aatgaacaac
     4201 gatcgcctga atccgggtga gcgttgtgcc tccaccagca accgtaactt tgaaggccgc
     4261 caggggcgtg gagggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg
     4321 accggacatt tcgccgacat tcgcaacatt aaataaggag cacaccatgg cagagaaatt
     4381 tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat
     4441 catcccgaaa cagtttttgc agaaagtgac ccgtacaggt tttggcgcgc atctgtttaa
     4501 cgactggcgt tttctggatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt
     4561 cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct gcggttcctc
     4621 gcgtgagcac gcgccatggg cattgaccga ttacggtttt aaagtggtga ttgccccgag
     4681 ttttgctgac atcttctacg gcaacagctt taacaaccag ctgctgccgg tgaaattaag
     4741 cgatgccgaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga ttcattttga
     4801 tgtagacctg gaagcgcaag aggtgaaagc gggagagaaa acgtatcgct ttaccatcga
     4861 tgccttccgc cgccactgca tgatgaacgg tctggacagc atcggcctga cattgcagca
     4921 cgacgacgcc attgcgtctt atgaagagaa gcaaccggca tttatgcgtt agtttgtaaa
     4981 ccgtccggca ctcaatcgcc aaaaagaaaa aagccagcag ataatcactg ctggcgtggc
     5041 gattaacacc aatactcagt cacacatgat gtccgcaaat cagtagtcag aatgactccc
     5101 ctcccactgc atcagttctt caagcatagt taatcgttgt gggacgctta accaacgcaa
     5161 ccagcacaac ttcgctgtcg cggtaaaata gtactgataa aactgacgca tggggcacct
     5221 ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt gctgtctttt
     5281 gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca tccgcctgtg
     5341 gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag cgttattgag
     5401 ctgctcgcgt cgtcatatgc gcaccctgct caacaccatg caggatcgcg gctggctgac
     5461 gtgggaagcg gaagttgggc gcggtaaacg ctcgcgtctg acattcctct acaccgggct
     5521 ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg atcaactagt
     5581 gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc tgggacgcag
     5641 cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc gtaatctgct
     5701 gcccggcagt gcattgcgcc gttccgaaac ccatatcgcc cggcaaatct tcagttcgct
     5761 aacgcgcata aatgaggaaa atggggaact ggaagcggac atcgcccacc actggcagca
     5821 aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc atcatggtcg
     5881 tgaactggaa atggacgatg tgatcgcctc tttaaaacgg atcaatacgc tgccgctcta
     5941 ttcgcatatt gctgaaattg tatcgccgac gccctggacg ctggatatcc acctcacgca
     6001 gccggaccgc tggttgccgt tactgctggg acaagttccg gcgatgatcc tgccgcgcga
     6061 atgggaaacc atcagtaact ttgccagcca tcccatcggc accggtccgt atggggtgat
     6121 ccgcaacagc accaatcaac tgaaaattca ggcattcgat gacttcttcg gttaccgggc
     6181 attaatcgac gaagttaacg tctgggttct accggaaatt gccgacgagc cagccggagg
     6241 gctgatgcta aaaggtccac agggcgagga aaaagagatt gaaagccgcc tggaggaagg
     6301 ttgctactat ttactgttcg acaaccgcac ccatcgcggg gcgaatcagc aagtcaggga
     6361 ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg aacagtacca
     6421 gcaactgtgg ttcccggctt atggactgct cccacgttgg caccatgctc gtccgacaca
     6481 ttgcgaaaaa cccgccgggc tggaaagcct caccctgacc ttttatcagg atcatattga
     6541 gcatcgagtg attgccggga tcatgcagca gattctggca agtcaccagg tcacgctgga
     6601 aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg atatctggct
     6661 taacagcgcc aactttacgc tgccgctgga tttttcgcta ttcgcgcacc tgtgcgaggt
     6721 gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc gctggcgcaa
     6781 tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagcaaag caatggtgcc
     6841 gcttatccac cactggctga ttattcaggg acaacgcagt atgcgcggcc tgcgtatgaa
     6901 caccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccgcctgatc cagagtagct
     6961 ctcgcctttt ggcggtgatg agcgcgtttt ttatattcag acggaatgaa taaaatacat
     7021 tacttttata ttttctctgt tttatgcatc gtctgattag agtcgcaaat aagattatct
     7081 cgaatcgtgt gcgattcatt gtctgaaaaa tgacgactcc gcactcactg aaaacaaggc
     7141 agagtctgga ttcgacttca gactccaact taaatttctt ttgccagaat aaaaatacac
     7201 gctcataatt tcagtaacac gctgttgttt gaggctaatc agacgagatt ttatcttgcg
     7261 tgggtgtggg atgttgatgt taagtgacag gcatatgaaa attagcatac cgggtaggat
     7321 tcagtatttc tgcgcaatat tgttttatat aaaaaataag tcagtgtgtt ttttacctta
     7381 ttttatatat agtggtcagc accaacttaa aaaagaaagg gcgttcctgc cccttcccta
     7441 tttattattc tggctcaatc aataattgcg ccacgactgc gcaatttagc gctatcctgg
     7501 ccggaaaggt cagccttgct caggttaatc ccgcgcacgt tagtatcttc catataagca
     7561 ttgttcagga tggtattgtc catttttgca ttgcgcagga gtgtatcgtc caggtcagta
     7621 tcggacatgt cagtatcgga caggttagta tcggacaggt cagagctaat aactttggca
     7681 ttgttcattt tagcaccgtg caggtcagca ccgagtaggt tagaactgca cagactgatc
     7741 ttgttcattt cagcattgct caggtcagcc ttgcgaaggc aagcaccatt aagggaaatc
     7801 ccggaaaggt aagcattact cagatcaatg cccccggtac tggggcttat tcccaattgc
     7861 aacttctgag tatatatctc tatatttcca atataaaaac tgacacataa catattatct
     7921 ggcgagattg agcccctcgc ctgcatcatt tccggatctt ttgcgtgttc tttgagttca
     7981 ttaaattttt ttagccggtg ggtaaaccta tcacgcgcct tctcgtcgac agccccggta
     8041 aagtattttg agtagtcatc acaattctgg aaataaaatg catccttcag aatttgcctt
     8101 gccgcagtct gggaatgttt atctgaccgg aagagattgc gtaacgtctc ccatagctct
     8161 tcaaagatat tcatattctc ggtctttttg accaggttaa ccatcctgat aatctgagaa
     8221 ttagtaaggc ctggatcttt agtggtatta cttgcaggga tactaatctg ttctgggttt
     8281 cttactttca tgctgatagc tccactctga gttaaaaact cagaataagc gtcctgaata
     8341 agaatactgt caaaaatcgc tcattcacgc aataagccgt tgcattaccg attttgactg
     8401 tgctaaggga ttctttcggt tcaaagcttc ccagctatgc aaatacgtcc ttgatagctg
     8461 aacgaatatt ggctttttta atacaccttc tctatggatt gctggttgat aacaaaatca
     8521 ctacactaac gccgttctca acggggtgcc acgcagacgc gtgcgctgag aaaatacccg
     8581 tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca agtcctttgc
     8641 cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca
     8701 gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt cgccgccgac
     8761 tgggggcctg gtccaaagat caaaaaagct tttgaagccg actgtaactg cgaactgaaa
     8821 ctggtggcgc tggaagatgg cgtttcgctg ctcaaccgtc tgcggatgga aggcaaaaac
     8881 agtaaagccg atgtggtgct ggggctggat aacaacctgt tagatgccgc cagtaaaacc
     8941 ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg cggctggaat
     9001 aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga caagaacaaa
     9061 ctgaaaaatc cgccgcaaag cctgaaagag ttggttgaaa gcgatcaaaa ctggcgggtg
     9121 atttatcagg atccgcgcac cagcacgccg ggactgggtc tgctgttgtg gatgcaaaaa
     9181 gtctatggcg atgacgcccc acaagcctgg cagaaactgg cgaagaaaac ggtcacggtc
     9241 accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga tctggtactg
     9301 agttacacca cctctccggc ttatcacatt ctcgaagaga agaaagataa ctacgccgcc
     9361 gcgaacttca gcgaaggtca ctatttgcaa gtggaagtcg ccgcccgcac cgctgccagc
     9421 aagcagccag agctggcgca aaaattcctc cagtttatgg tttctccggc ttttcagaat
     9481 gcgatcccaa ccggcaactg gatgtatccg gtggcaaacg tcacgctgcc tgccggtttt
     9541 gaacaattga ccaaaccggc aaccacgctg gagttcacgc cagccgaagt ggcggcacaa
     9601 cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc ggctggttaa
     9661 ttccaggtgt aagcgccgct acgcttgtgg tggcggttgc gctggcggcg tttctcgccc
     9721 tgtggtggaa cgcgccgcag ggtgactggt cggcagtctg gcaggacagc tacctgtggc
     9781 atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct gtcgtacccg
     9841 cgatattcct cgcccgcgcg ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc
     9901 gtctgtgcgc aatgaccttg atcctcccgg tgctggttgc tgttttcggc attcttagcg
     9961 tctatggtcg ccagggctgg ctggcatcgc tctgccaatc gctcggtctg gagtggacct
    10021 tttcgcccta cggcctgcaa ggcattttgc tggcccatgt gttttttaat ctgccgatgg
    10081 cgagccgttt attacttcag gcactggaaa acattcccgg cgaacaacgt caacttgccg
    10141 cccagcttgg gatgcgtggc tggcacttct ttcgtttcgt cgaatggccg tggttacggc
    10201 gacaaatccc gccagttgct gcgcttatct ttatgctctg tttcgccagc ttcgccaccg
    10261 tgttgtcgct ggggggcggt ccgcaggcga ccactatcga actggctatt tatcaggcgc
    10321 tgagttacga ctacgatccc acccgcgcgg cgatgttggc gctgatccag atggtgtgtt
    10381 gccttgggct ggtgctgctg agtcagcgat tgagtaaggc cattgcgcca ggcaccacgc
    10441 tgctgcaagg ctggcgcgac ccggacgatc gtctgcatag ccgcatttgc gacacggtgt
    10501 taattgtgct ggcgctgctg ctattgctgc caccgttact ggcggtgctc gtcgatggga
    10561 taaatcgcca gttgccggaa gtgctggcgc agccggtgct gtggcaggcg ctgtggacct
    10621 cgttgcgtat tgcgctggcg gcaggcgtat tgtgcgtagt gctgaccatg atgctgctat
    10681 ggagcagtcg cgaacttcgg gcgcggcaga aaatgctggc cggtcaggcg ctggaaatga
    10741 gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc tttttactgc
    10801 tcaacaacac tatcggcctg ccgcaatctg ctgacggcat tgtgattttc accaatgcgt
    10861 taatggcgat cccttatgcg ctgaaggtgc tggaaaaccc gatgcgcgat atcaccgcgc
    10921 gttacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcacgctta aaagtggtgg
    10981 agctgcgcgc cctgaaacgc ccacttgcgc aggcactggc ctttgcttgt gtgctatcga
    11041 ttggtgattt tggcgtggtg gcgttgttcg gtaacgatga tttccgcacc ctgccgtttt
    11101 atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc gcgttaattc
    11161 tgctgctact ctgttttctg ctgtttaccg tgattgaaaa actaccgggg cgaaatgtta
    11221 aaactgactg atatcacctg gctttaccac catttgccga tgcgttttag cttaacggtg
    11281 gaacgaggcg agcaggtggc gatcctcggg ccaagcggcg cgggtaaaag taccctgctg
    11341 aatttgatcg ccggttttct gacgccagcc agcggttcgc tgactatcga cggcgttgat
    11401 cacacctcta cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga gaacaacctg
    11461 ttcagtcacc tgacggtcgc gcagaacatc gggctggggc ttaatccagg attgaagctg
    11521 aacgcggcac aacaggagaa aatgcacgct atcgcccgcc agatggggat tgataattta
    11581 atggcgcgat taccgggcga gctttctggc ggtcagcgac agcgcgtggc gttagcgcgt
    11641 tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg
    11701 gcgttacgtc aagagatgtt gacgctggtg agcacgagct gccagcagca aaaaatgacg
    11761 ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg ctcggtggtg
    11821 gtcgccgacg ggcgcatcgc ctggcagggt atgaccgatg agttgttgag cggtaaggcg
    11881 agagcttcgg cactattggg gattacgggt tagtatgcac tgtcggatgc ggcgtgaacg
    11941 ccttatccga cctacggaca gtgcgttttg taggcctgat aagacgcggc aagcgtcgca
    12001 tcaggcaacc cgcacaaggc tatcccctaa cccccaacca ctttacgcaa aatatcgata
    12061 tacatcggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa
    12121 atcaacggcg tcagccacaa caaacgaccg cgcgacaaat aatgacttaa acgatcggtt
    12181 gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc
    12241 gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc
    12301 gcacccgcca gaatccccgg caggaagtaa aacggtggcc acagcaggca gccgataata
    12361 ttcggcgtaa taaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc
    12421 ggacgcgtcg ggccaacaaa acgaccgacc agaatggtga acatgctgtg ttgatgcaac
    12481 gcatgttcag ttttatcaag tagtgctttg tttttcttca gaaatgacca gcgatgcaat
    12541 ggctttttaa aacgccagcc cagccagaaa gagatccagt cgcccagcag gcaaccgacg
    12601 atccctgcca gccaggcgtg ccagaaactt aactcgccgc tgccaatcag cgcccccagc
    12661 cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag
    12721 gccaccaaca ccaccgctat caatgaatac acggtggatt gggtaataaa gtgttccagc
    12781 aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
    12841 ctccgtcaag tcgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
    12901 attcactttt tcttcacaac cggcacggaa ttcactcggg ctggccccgg tgcatttttt
    12961 aaatactcgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtagcgat
    13021 aggcatccgg gtggtgctca aaagcagctt cgcctgacta atgcgttggt cctcgcgcca
    13081 gcttaagacg ctaattccta actgctggcg gaacagatgc gacagacgcg acggcgacag
    13141 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
    13201 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaattgc
    13261 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
    13321 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggttggtg
    13381 cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc
    13441 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
    13501 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
    13561 gacaaattct cgtccctgat ttttcaccac cccctggccg cgtatggtga gattgagaat
    13621 ataacccttc attcccagtg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
    13681 cggcgttaaa cccgccacca gatgggcgtt aaacgagtat cccggcagca ggggatcatt
    13741 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
    13801 ttgcatcaga cattgccgtc accgcgtctt ttactggctc ttctcgctaa tccaaccggt
    13861 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
    13921 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
    13981 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
    14041 ttcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga
    14101 ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca
    14161 ccggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcagaaa gggcaatttt
    14221 gtgatgcccc aaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag
    14281 cggcgctgaa aaccgtgctt gcagagctta gcgccgagca gcgcgcagct gtggtcggga
    14341 ttggcgttga cacaaccggc tcgacgcccg caccgattga cgcggacggt aacgtcctgg
    14401 cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg aaagaccaca
    14461 ccgcggttga agaagcggaa gagattaccc gcttatgtca cacgccgggc aacgttgact
    14521 actcccgcta tattggcggt atttattcca gcgaatggtt ctgggcaaaa atactgcatg
    14581 taactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact
    14641 gggtgccagc tctgctttcc ggtaccacgc gcccgcagga tattcgtcgc ggacgttgca
    14701 gcgccgggca taagtcgcta tggcacgaaa gctggggcgg cctgccgcca gccagtttct
    14761 ttgacgaact ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgatacct
    14821 ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc
    14881 ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag
    14941 gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg
    15001 ccgacaaaca gagcgttggc gagcgggcag tgaaaggtat ttgcggccag gttgatggca
    15061 gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggc gatatctacg
    15121 cctggttcgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag catccggaac
    15181 tgaaagagca aatcaacgcc agccagaaac aactgcttcc agcgctgacc gaagcatggg
    15241 ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac ggccgccgca
    15301 caccgaacgc taaccaacgc ctgaaagggg tgattaccga tctgaacctc gctaccgacg
    15361 ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg
    15421 agtgctttac cgatcagggg atcgccgtta ataacgtgat ggcgctgggc ggcatcgcgc
    15481 ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg
    15541 ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt cgctgccgtc gccgcgaaag
    15601 tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtcgag aaaaccctgc
    15661 aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat cagcaatggg
    15721 cgatgagcgc cgaacaacat tatcttccaa cttccgcccc ggcacaggct gcccaggccg
    15781 ttccgactct ataaggacac gacaatgacg atttttgata attatgaagt gtggtttgta
    15841 attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag
    15901 cacgtcgtta atgcgctgaa tacggaagcg aaactgccct gcaaactggt gctgaaaccg
    15961 ctgggcacca cgccggatga aatcaccgct atttgccgtg acgcgaatta cgacgatcgt
    16021 tgcgccggcc tggtagtgtg gctgcacact ttctccccgg ccaaaatgtg gatcaacggc
    16081 ctgactatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc ggcgctgccg
    16141 tgggatagca tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag
    16201 ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg tcactggcag
    16261 gataaacaag tacatgagcg tatcggctcc tggatgcgtc aggcggtatc taaacaggat
    16321 acccgtcatc tgaaagtctg ccgttttggc gataacatgc gtgaagtggc ggtcaccgat
    16381 ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg ggcggttggc
    16441 gatctggtgc aggtagtgaa ctccatcagc gatggcgatg ttaacgcgct ggtcgatgag
    16501 tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgccagaac
    16561 gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca aggtggcttc
    16621 cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc tggtctggcc
    16681 gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaactgcc
    16741 gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg
    16801 gaggactaca cctatcactt cgagaaaggc aatgacctgg tgctcggctc ccacatgctg
    16861 gaagtctgcc cgtcgatcgc cgcagaagag aaaccgatcc tcgacgttca gcatctcggt
    16921 attggtggta aggacgatcc tgcccgcctg atcttcaata cccaaaccgg cccagccatt
    16981 gtcgccagct tgattgatct cggcgatcgt taccgtctgc tggttaactg catcgacaca
    17041 gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcgctgtg gaaagcgcaa
    17101 ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca ccataccgtt
    17161 ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca cgacattgaa
    17221 atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcgct gcgctggaac
    17281 gaagtgtatt acgggtttcg tcgctaagta gccgcatcag gtgtgtaacg cctgatgcga
    17341 cgctgacgcg tcttatcagg cctacacgct gtgattttgt aggccggata agcaaagagc
    17401 atccggcatt gttttccccc tcgccccttt ggggagaggg ccggggtgag gggaaacgac
    17461 ctctcaggag tcaacatgtt agaagatctc aaacgccagg tattagaagc caatttggcg
    17521 ctgccaaaac ataacctggt cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag
    17581 cgcggcgtct ttgtgatcaa accttccggc gtcgattaca acgtcatgac cgctgacgat
    17641 atggtcgtgg tcagcatcga aaccggtgaa gtggttgaag gaacgaaaaa gccctcctcc
    17701 gacacgccaa ctcaccggct gctctatcag gcattcccgt ccattggcgg cattgtgcac
    17761 acacactcgc gccacgccac catctgggcg caggcgggtc agtcgattcc agcaaccggt
    17821 accacccacg ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca
    17881 gaaatcaacg gtgaatatga gtgggaaacc ggtaacgtca tcgtagaaac cttcgaaaaa
    17941 cagggtatcg acgcagcaca aatgcccggc gtcctggtcc attctcacgg cccgtttgca
    18001 tggggcaaaa atgccgaaga tgcggtgcac aacgccatcg tgctggaaga agtcgcttat
    18061 atggggattt tctgccgcca gcttgctccg cagttaccgg atatgcagca aacgttgctg
    18121 gataaacatt atttacgtaa gcatggtgcg aaggcatatt acgggcagta ataactgtat
    18181 aaaaccacag ccaatcaaac gaaaccaggc tataatcaag cctggttttt tattggattt
    18241 ttagcgtggc gcaggcaggt tttatcttaa cccgacactg gcgggatacc ccgcaaggaa
    18301 ccgaagtttc cttttggtta gcgacggaca acgggccgtt gcaggttacg cttgcaccgc
    18361 aagagtccgt ggcctttatt cccgccgatc aggttccccg cgcccagcat attttgcagg
    18421 gtgagcaagg ctttcgcctg acgccgctgg cgttaaagga ttttcatcgc cagccggtgt
    18481 atggccttta ctgtcgcgcc catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag
    18541 gtggcgttac catctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt
    18601 ttatcacctc accggtgtgg gtcgagggtg atatacgcaa tggcgctatc gttaatgccc
    18661 gtctgaaacc gcatcccgac taccgtccgc cgctcaagtg ggtttctatt gatattgaaa
    18721 ccacccgcca cggtgagctg tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt
    18781 atatgctggg gccagagaat ggcgacgcct ccgcgctcga ttttaaactg gaatatgtcg
    18841 ccagtcgccc gcagctactg gaaaaactca acgcctggtt tgcgactcac gatcccgatg
    18901 tgatcatcgg ctggaacgtg gtgcaattcg atctacgaat gctgcaaaaa catgccgagc
    18961 gttatcgtat tccgctgcgg ctggggcgcg ataacagtga gctggagtgg cgcgagcacg
    19021 gctttaaaaa cggcgtcttt tttgcccagg ccaaagggcg actgattatc gacggtatcg
    19081 aggcgctgaa atccgcgttc tggaatttct cttcattctc gctggaaacc gtcgctcagg
    19141 agttattagg cgaaggaaaa tctatcgata acccgtggga tcggatggac gaaattgacc
    19201 gccgtttcgc cgaagataaa cctgcgctgg caacttataa cctgaaagat tgcgagctgg
    19261 tgacgcagat cttccacaaa actgaaatca tgccgttttt actcgaacga gcgacggtga
    19321 acggcctgcc ggtagatcgg cacggggggt cggtggcagc gtttggtcat ctctattttc
    19381 cccgaatgca tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca
    19441 gccctggcgg ctatgtgatg gattcgcggc ctgggcttta tgattcggtg ctggtgctgg
    19501 actacaaaag cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcggactgg
    19561 tggaaggcat ggcgcagcct gatccagagc acagcaccga aggctttctc gatgcctggt
    19621 tctcgcggga gaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg
    19681 aagccaaacg tcagggtaat aagccgcttt cgcaggcgct gaagatcatt atgaatgcct
    19741 tttatggcgt gcttggcacc accgcctgcc gcttcttcga tccgcgcctg gcgtcgtcga
    19801 tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc gttgattgaa gcgcagggct
    19861 acgacgtgat ctacggcgat accgactcaa cgtttgtctg gttgaaaggc gcacattcgg
    19921 aagaagaagc ggcgaaaatt ggtcgtacac tggtgcagca cgttaacgcc tggtgggcag
    19981 aaacgctgca aaaacaacga ctgaccagcg cactggaact ggagtatgaa acccatttct
    20041 gccgttttct gatgccaacc attcgcggag ccgataccgg cagtaaaaag cgttatgccg
    20101 ggctgattca ggagggcgat aaacagcgga tggtgtttaa agggctggaa accgtgcgca
    20161 ccgactggac gccgctggcc cagcagtttc agcaggagct gtacctgcgt atcttccgca
    20221 acgagccata tcaggagtac gtccgcgaaa ccatcgacaa actgatggcg ggtgaactgg
    20281 atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat cagcgtaatg
    20341 ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc
    20401 ccttgcaata tcagaaccgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc
    20461 cgctggacta ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac
    20521 ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc
    20581 aacttgggct attttgagca aaaaaaagag ttcgccagat accattttga tgcgtgacga
    20641 atgctttgcc atccagtacc atagcgccct ttccattcct ggacctgaat aacaccacta
    20701 cctcataagc acggtagcgg gtggttattg cctgcaatta aagatataga gccgaacaca
    20761 tatgcctttt acacttggtc aacgctggat cagcgataca gaaagcgaat tgggacttgg
    20821 aaccgttgtc gcggtggatg cgcgaactgt cactttactt ttcccatcta ctggtgaaaa
    20881 ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg atgttcaacc ctggtgatac
    20941 cattaccagc catgacggct ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt
    21001 gctgacctat atcggtactc gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt
    21061 tttccttgat agcaaactgg tgttcagcaa accgcaggac cgtctgtttg ccgggcagat
    21121 tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt
    21181 ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc ctgatcccgc accagctcaa
    21241 catcgctcat gatgttggcc gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg
    21301 tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc
    21361 tgaacgtgtg ctgattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct
    21421 gcgccgtttc aacctgcgct ttgctctgtt tgatgatgag cgttatgccg aagctcagca
    21481 cgatgcttac aacccgttcg ataccgaaca gctggtgatt tgctcgctgg attttgcccg
    21541 tcgtagcaaa cagcgtctgg aacatctctg tgaagccgaa tgggatctgc tggtggtcga
    21601 tgaagcccat cacctggtgt ggagcgaaga tgcgccgagc cgcgaatatc aggccattga
    21661 acaactggca gagcatgtgc cgggcgttct gctgctgacc gcaaccccgg aacagctggg
    21721 gatggaaagc cacttcgccc gtctgcgtct gctggacccg aaccgtttcc acgattttgc
    21781 gcaattcgtt gaagagcaga aaaattatcg tccggttgcg gacgccgttg ccatgctgct
    21841 ggcaggtaac aagctgagca atgacgaact gaacatgctt ggcgagatga tcggcgagca
    21901 ggatatcgag ccgctgttac aagcagcaaa cagcgacagc gaagatgccc agagcgcccg
    21961 tcaggagctg gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa
    22021 cacgcgtaac ggtgtgaaag gcttcccgaa acgcgagctg cacaccatta agctgccgct
    22081 gccgacgcag tatcagacgg ctattaaagt ctccggcatt atgggcgcac gcaaaagtgc
    22141 ggaagatcgc gctcgcgata tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga
    22201 taacgccacc tggtggaact tcgatccgcg cgttgagtgg ctgatgggct acctgaccag
    22261 ccatcgctct cagaaagtgc tggtgatctg cgctaaagct gccactgcgc tgcaactgga
    22321 gcaagtactg cgcgaacgtg aaggtatccg cgctgctgtg ttccacgaag gcatgtcgat
    22381 tattgagcgt gaccgcgctg ccgcctggtt tgctgaagaa gacaccggtg cgcaggtact
    22441 gctgtgctcg gaaatcggtt ctgaaggacg taacttccag ttcgccagcc acatggtgat
    22501 gtttgacctg ccattcaacc cggatctgct ggagcagcgt attggtcgtc tggatcgtat
    22561 cggccaggcg cacgatattc agatccatgt gccttatctg gagaaaaccg ctcagtcggt
    22621 gctggtgcgc tggtatcacg aaggtctgga tgcattcgag cacacctgcc cgaccggacg
    22681 cactatttac gatagcgtat acaacgatct gattaactat ctggcttcac cggatcaaac
    22741 cgaaggcttt gacgatctga tcaaaaactg ccgcgagcag cacgaagccc tgaaagcaca
    22801 attggaacag ggccgtgacc gcctgctgga gatccattcc aacggtggcg aaaaagccca
    22861 ggcactggca gaaagcattg aagagcagga tgacgatacc aacctgatcg ccttcgccat
    22921 gaacctgttc gatattatcg gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct
    22981 gacaccgtcc gatcatatgc tggtgccgga cttccctggc ttgtcggaag atggcatcac
    23041 catcaccttc gaccgtgaag tggcgctggc gcgtgaagat gcgcagttta ttacctggga
    23101 gcatccgctg atccgcaacg gtctggatct gatcctttct ggcgataccg gtagcagcac
    23161 gatttcactg ttgaaaaaca aagcgttgcc ggtaggtacg ctgttggtgg aactgattta
    23221 cgtggtcgaa gcccaggctc cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc
    23281 ggtacgtatg ctgctggata aaaacggcaa caacctggcg gcgcaggtgg agtttgaaac
    23341 ctttaaccgt cagcttaacg cggttaaccg tcacaccggc agcaaactgg ttaacgccgt
    23401 gcagcaggat gttcacgcta tccttcagtt gggtgaagca cagatcgaga aatctgcccg
    23461 tgcactgatt gatgccgcac gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg
    23521 tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac gacgaactga ccgccattga
    23581 gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca ggctggcgtc tggatgccct
    23641 gcgtttgatc gttgtgacgc atcagtaacg gagccgaaaa tggggatgga aaactacaat
    23701 ccaccgcagg aaccctggtt ggttatcctg tatcaggatg accacattat ggtggtcaac
    23761 aagccgagcg gtttgttgtc tgtgccgggt cgtctggaag agcacaaaga cagcgtgatg
    23821 acgcgcattc agcgtgatta tccgcaggcg gaatcggtgc atcgtctgga tatggctacc
    23881 agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa acgccagttc
    23941 cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc atcccctgcg
    24001 gaaggtctgg tagatctgcc gctgatttgc gactggccaa accgcccgaa acagaaagtc
    24061 tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag tggtggagta tgcggcggat
    24121 aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat
    24181 atgctggcgc tgggtcatcc gattctcggc gatcgttttt atgcatcacc ggaagcgaga
    24241 gcgatggcac cgcgtttgtt gctacatgca gagatgttga cgattaccca tccggcgtat
    24301 ggcaatagta tgacgtttaa agcgccagcg gatttttaac ctttcctgta cgtttatacc
    24361 ctcatcctaa gggctgcctc ttgtacataa atccccgagt ccggggattt tttgtttctg
    24421 ccaaactgct ttcagttgtt gcgtgtggct acgctttcca ttcggtaatt cccttcctgc
    24481 tccacagcga atttttgaac tttttcttca cccgttgatt gtctctatta tttcatttta
    24541 tcgggtctgt tatggtcctt tctgattgtt attcatgggc taattagcag tctggttggc
    24601 gatgcccatc tcggtgatcc gcgtcgtgca cgacgccttg catcaccagc ctcttctcta
    24661 gctcatcatg caggtctttc taattgtgaa atcatcccac gctactgcta aggttgaagg
    24721 ggcgttccag ccccttcaac cttagcttat tctggatcat tcaataattg cgttggcaat
    24781 aatttttcag cagtactttg tttagtagca actccttcag taacaatttt ttttggggtg
    24841 attttattaa taataattac ctcagcatat aggcgggtag caccgtccac ggtagcaccg
    24901 tccaggatag caccgtccag ggtagcaccg tccaggttag cattccaccc aggtttcatg
    24961 taaaggtttt cattgctatc tattattgtt tttttaccaa agtaacaatc cctcatgtca
    25021 acgccgggaa ggtaagcctc tccaagatca acgcccaagt tacctgggcc tttatgcagc
    25081 tgcaacgtct gagtacatat ctccttattt tcaatgaaaa aagtgacact taactcgata
    25141 tcaccgtaga tctcggcgac ccagcgcatc ttgccctttg cgaccatctc atccggattt
    25201 tttgcgcatg ttttgagttc attgaataat tccagactat ggataaactt ttctttctca
    25261 ttctcgttga cagccccagt aaagtatttt gaggcgttga tgttgccagc ctttttattc
    25321 acgaaattga acagaagttt ataagcctca gtctgtttat ctgaccagaa cagatgacgt
    25381 aacgtctccc acaagcgttg aaagatattc atcttcacgt ccatcttgtc cccgctcacc
    25441 atcttataaa tatcgttatt gctcatattt aaaagtgtag ccggcatgcc tggaactgat
    25501 actttcatgg taatagcctc gcattgagtt aaaaactcat gatgcgcgct ctgaataaat
    25561 atactttcaa aaatcgctca ttcagatatg tgtttttaat ttttaagacg taaaaatata
    25621 attccgctcg tcgtaaagct ctcaacctta agcagggtca acgcgataaa accaacagaa
    25681 accctcccgt aaaccgtgta ttgagcctgt ccaccactct gagtaaacgc cttttctcaa
    25741 agaagtgacc gccagggcgg ccacctttaa tcgtgggcag ttactcagca tcatttacag
    25801 ggtcatttaa accctttctg ctgttttatc agctcatagg cctgctgaat ttcctgcgct
    25861 ttctgcttcg ccatctccat catctctggc ggcaaacctt tcgccaccag cttatcggga
    25921 tggtgttcac tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc
    25981 ttcacgccca gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag
    26041 ttaccaccgc cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg
    26101 cgcaaaaact ggtcaaactg ggcgcgggag atgcctaatt cttcagcaat aacatacagc
    26161 accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc
    26221 agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt
    26281 tcgcgcagcg ggtaattgtc tgattttccc acccggaacg cgttttgcgc cgcagtacgg
    26341 gaagcgccat gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcctcc
    26401 gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac
    26461 agcgcctgac gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca
    26521 aacatatggc caattaacag gcctaacact acgccccaaa agccgccgcc catcagtaag
    26581 gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg
    26641 gatatcaggg ctatatccca caatataaag gtgcttttac cgttttccgg ctgcggtcaa
    26701 ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca
    26761 ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc
    26821 tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa
    26881 acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact
    26941 ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt
    27001 acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta
    27061 cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca
    27121 ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac
    27181 cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa
    27241 aggctgggcg aatctgaaca ccaaagatac caatgtctgg gaaggtgatt accagatggt
    27301 gggtcgtcag ggccgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac
    27361 cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt
    27421 aggtagcgaa attattcatg accgcgaaga acaagttgcg gagatctgga acgcccgctt
    27481 taaggtgggt ccggtaccga tcttttatag cccctatttg cagttgccgg tgggtgacaa
    27541 acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact actttgagtt
    27601 ctaccttcca tattactgga acatcgcgcc aaatatggat gccaccatca cgccgcatta
    27661 tatgcatcgt cgtggcaata tcatgtggga gaacgaattc cgctacctct cccaggcggg
    27721 cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag atgaacaccc
    27781 gaacgatgac agttcacgtc gttggttatt ctactggaac cactccgggg tcatggatca
    27841 ggtgtggcgt ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgattt
    27901 cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca gcgttggcta
    27961 tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggtct tcagcgagca
    28021 gaacaccagt agctactcgg cagagccgca gttagacgtt aactactacc agaatgatgt
    28081 tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca ccagagacga
    28141 catgcctgaa gcaacccgtg ttcacctgga accgaccatc aatttgccgc tctctaataa
    28201 ctggggcagc atcaataccg aagcgaagtt gctggcaacc cattatcagc aaactaatct
    28261 tgactggtat aactccagaa acacaaccaa gctggacgaa tccgttaacc gcgtaatgcc
    28321 gcaatttaaa gttgacggca aaatggtctt tgaacgcgat atggaaatgc tggctccggg
    28381 ttatacccaa acgctggaac cgcgcgcgca gtatttatac gtgccgtatc gcgatcagag
    28441 cgacatctat aactacgact cgtctctgct gcaatctgac tactctggcc tgttccggga
    28501 ccggacttac ggcggtcttg accgtattgc ctccgctaac caggtgacga ccggtgtcac
    28561 atctcgcata tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta
    28621 ctatttcacg gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac
    28681 gggctcactg gtgtgggcag gcgatactta ctggcgtatc tccgagcgtt ggggattgcg
    28741 tggcgggatt cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga
    28801 ataccgtcgg gatgaagacc gtctggtaca gctgaattac cgttacgcca gcccggaata
    28861 tattcaggct acgctgccta agtactattc cacagctgag caatataaga atggtatttc
    28921 gcaggtaggt gccgtcgcca gctggccaat tgctgatcgt tggtcaattg ttggggccta
    28981 ctactacgac accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc
    29041 ctgctgctat gcaattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa
    29101 acaacatgcg gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc
    29161 caactacggt ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac
    29221 tttgtgattt gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta
    29281 tgaagaactg gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg
    29341 ccccccaggt agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg
    29401 acgttgatgg attaatgcag tcggtaaaac tgaacgctgc tcaggcaagg cagcaacttc
    29461 ctgatgacgc gacgctgcgc caccaaatca tggaacgttt gatcatggat caaatcatcc
    29521 tgcagatggg gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg
    29581 ctaacatcgc gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg
    29641 atggtctgaa ctacaacacc tatcgtaacc agatccgcaa agagatgatt atctctgaag
    29701 tgcgtaacaa cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg
    29761 cgcagcaggt gggtaaccaa aacgacgcca gcactgagtt gaacctgagc cacatcctga
    29821 tcccgctgcc ggaaaacccg acttctgatc aagtgaacga agcggaaagc caggcgcgtg
    29881 ccattgtcga tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg
    29941 ccgaccagca ggcactgaac ggcggccaga tgggctgggg ccgtattcag gagttgccgg
    30001 ggatcttcgc ccaggcatta agcaccgcga agaaaggcga cattgttggc ccaattcgtt
    30061 ccggcgttgg cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct
    30121 cggtgaccga agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg
    30181 aacaggcccg cgtgaaactg gaacagattg ctgccgatat caagagtggt aaaacgactt
    30241 ttgctgccgc agcgaaagag ttctctcagg atccaggctc tgctaaccag ggcggtgatc
    30301 tcggctgggc tacaccagat attttcgatc cggccttccg tgacgccctg acccgcctga
    30361 acaaaggtca aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc
    30421 tggatacccg taatgtcgat aaaaccgacg cggcacagaa agatcgtgcg taccgcatgc
    30481 tgatgaaccg taagttctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg
    30541 cctacgttaa aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg
    30601 cgagcccgcc gggattggcc cggacttagt tgtccagctt gcacagcgtg agtggccggt
    30661 cgaactggtt gtttgtgccg atgccactct ccttaccaac cgggcagcga tgctcggttt
    30721 gccgctcacc ctccgccctt attcccccaa ctcccctgca caaccgcaaa ctgcgggcac
    30781 attaacgcta cttcctgtcg cgctacgtga atctgtcact gcggggcagt tagcggttga
    30841 aaatgggcat tatgtggtgg aaacgctggc gcgagcgtgt gatggctgtc tgaacggtga
    30901 atttgctgcg ctgatcacag gccccgtgca taaaggcgtc attaacgacg caggcattcc
    30961 gtttaccggt cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat
    31021 gctggcgacc gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat
    31081 cgcagatgct atcacccctg cgcttttgca cgaagtgatt gctattttgc atcacgattt
    31141 gcggaccaaa tttggtattg ccgaaccgcg cattctggtc tgcgggctga atccgcacgc
    31201 gggcgaaggc ggtcatatgg gtacggaaga gatagacacc attattccgg tgctcgacga
    31261 gctgcgggcg caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc
    31321 gaaatatctc gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt
    31381 gctaaaatac cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg
    31441 cacatcagtg gaccacggca ccgcgcttga actggcggga cgtggcaaag ccgatgtcgg
    31501 cagttttatt acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat
    31561 cgagtccacc agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag
    31621 ttcgtgatcg acagcattgt ttctgccatt aacccgcaga agggccaggc gatggtcgaa
    31681 atcggccccg gtctggcggc attgaccgaa ccggtcggcg aacgtctgga taagctcacg
    31741 gtcatcgaac ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg
    31801 aaactgacta tttatcagca ggatgcgatg acctttaact ttggtgaact ggccgagaaa
    31861 atgggtcagc cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg
    31921 ttccatctgt ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagag
    31981 gtggtgaatc gtctggttgc aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg
    32041 gcgcaatact attgcaacgt gatcccagtg ctggaagtac cgccgtcagc ctttacacca
    32101 ccacccaaag tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg
    32161 gttaaagatg ttcgcgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa
    32221 accattcgta acagcctcgg caacctgttt agcgtcgagg tgttaaccgg aatggggatc
    32281 gacccggcga tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat
    32341 ctggcggaga acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta
    32401 ttcaggttca aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt
    32461 ttgcttatac cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt
    32521 actggctgat caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg
    32581 gcgtccagcc acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg
    32641 aaaccccgct gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt
    32701 tcagcatcga cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata
    32761 tggcgacata ccttattggc gacgttcatg gttgttacga tgaactgatc gcattgctgc
    32821 ataaagtaga atttacccct gggaaagata ccctctggct gacgggcgat ctggtcgcgc
    32881 gcggccccgg ttcactggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc
    32941 tggtgctggg caatcacgat ctgcatctgc tggcggtatt cgccgggatc agccgcaata
    33001 aaccgaaaga tcgcctgaca ccgctgctgg aagctccgga tgccgacgag ctgcttaact
    33061 ggctgcgtcg ccagcctctg ctgcaaatcg acgaagagaa aaagttggtg atggcccacg
    33121 ccgggatcac gccgcagtgg gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag
    33181 cggtgctgtc gagtgactcc tatcccttct tccttgatgc catgtacggc gatatgccaa
    33241 ataactggtc accggaattg cgggggctgg gaagactgcg gtttatcacc aatgcattta
    33301 cccggatgcg tttttgcttc ccgaacgggc aactggatat gtacagcaaa gaatcgccgg
    33361 aagaagcccc tgccccactg aaaccgtggt ttgcgattcc tggccctgtc gctgaagagt
    33421 acagcatcgc ctttggtcac tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt
    33481 acgcgctgga tactggctgc tgctggggtg gtacattaac ctgcctgcgc tgggaagata
    33541 aacagtattt tgtccagccg tcgaaccggc ataaagatat gggtgaggga gaggcggccg
    33601 cgtcttaaat cccgtcgagc ataaaatcta atgctccttt aatcaacgtt cggtgcgcgc
    33661 tggcatcgca aaatttcgtt cccagcgcgt taactggagc agttgcagtc tcatgcgtca
    33721 tgagccgtct accagtaaaa ggattgtgtt aagccgttct ggagggcgaa tacggataaa
    33781 tcgttcaata ggtgttaatg gaataacaat actgcgatta atctcaccaa taatgtcgct
    33841 ggtaacatcg ataacaaagg gaaacgcggc gttactgccg cgactgcggt ataccgtaaa
    33901 ttgcatcgct agaagctccg gtactcatca gagaaacatc cggtttcatc agccaattga
    33961 tttaacgcag cgatagcttc tgcgttctct tcacgccaac gtgttgctgc atgtttttta
    34021 agttcagcat tgagcgcaac cgtaagggtg gcgctcatat ttaagcctgc atcacgagcg
    34081 cgattgacta actcacggtc gacggtgacg gtcacacttt gtgtggtacg ttttgcagtc
    34141 atgatatcgt gctcctgtac aatttatacg tctagtaaac acggatatct gcacgtatca
    34201 gtgatgccgg aattctaata tacaaactta ccgccgctcc agaatctcaa agcaatagct
    34261 atgagagttc tgcgcatcag cgtcgtggaa ttcgctgaat accgattccc agtcatccgg
    34321 ctcgtaatcc gggaaatggg tgtcgccttc cacttctgcg tcgatatgcg tcagatacag
    34381 tttttgcgct ttgggcagga actgctcata aacgcgacca ccgccaatca ccatgatttc
    34441 tggtacgtca ccacacgccg cgatggcttc atcaaccgat ttcacccacg taacgcgatc
    34501 gtcagtaccc ggttgactgc tgaggataat atttttgcgt cctggcagcg gacgaccgat
    34561 ggattcccag gtatggcggc ccataattac gggtttattt aaagtgttgc gtttaaacca
    34621 ggcgagatcg gcaggcaggt tccacggcat ggcgttttcc atgccgataa cgcgatctac
    34681 cgctaacgcc gcaatcagac tgatcattga gatttcccga taaaaaaaat tgtcgccact
    34741 atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga aaattttata
    34801 ttctgctggc gagtccacgc tctctccctg gactcgccgc attacaatga aacaaaaaca
    34861 aacagttagc tgtaaagtgt gatttacgtc actctttatt aggatgaggg tttcgtttcc
    34921 ggttcatccg ccatgttgcc ggtatgttta ccttcttctg ttccctgcca gccatgtcgt
    34981 tgaattaatg acagatgttc gcggtcctcg gtaatgatct cacttaacat cgcgctggtg
    35041 cgtttataga ccgccgcgcg ggctttggtg tcgttctcaa ccattgccat ctcttccacc
    35101 atctgaatat taaagcggcg gaacacatcg gcacgttctc gcgcttcata cggccccaga
    35161 cctaaacttt ccagtgccag acgcccggtt ttcagcgcac cttcaaaggt ttctcgctct
    35221 ggcttttcaa cgcctgcctg acgcaaacga atgtagtgat cgacatcgcg ggcgcgggca
    35281 ataatctgca aatgcgggaa atgttctttc accatctccg tcagttgcag gttggtttgc
    35341 ggatcgtcga tggcgttaat cagcacttcc gctttcgccg ctccggcaga ttccagtaaa
    35401 tccatccgcg tggcatcgcc ataaaacact ttcataccaa atttacgcaa ggtttcgata
    35461 tggtccggat cgtgatcgag caccaccatt ttcaccccgc tggagagcag taaacgtccg
    35521 gtgatctgcc caaaacgacc gaatccggca atgattaccc gcggctgttc ttcgtcgatc
    35581 tcatcggctt cacgcgcctc ttcagtagaa gattgctcaa ggcgattgag gatcaccagc
    35641 agaatcggcg ttgccgccat cgacagcgcc accgccaggg tcagcgattt cgcccactcc
    35701 ggctccagca cattcgccat ctgcgccgca ccaaatacca caaaggcaaa ctcactaccc
    35761 tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg gcacctgcaa cggtcgggca
    35821 atcagccaca acatggcgat tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc
    35881 aatggatttt caagcagcgt gccaaagtct atcgacatgc caacaccgat gaaaaacagc
    35941 cccagcagca aacctttaaa cggttcgata tcgctttcca gcgcatgacg gtattccgag
    36001 cttgccagca gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc
    36061 agcagcaaac caaagccaaa cacgaggaat aacgccacgg cactaaacac ttcccgcaag
    36121 ccagagcggg caacaaaacg cagcgccgga cgcgtgacat agcgccccag caataccacc
    36181 agcaccagcg cacccgccac ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag
    36241 gcactgctcg ctgccagtag cgggatcatc gccaccagcg ggatcgccgc gatatcctgg
    36301 aacagcagca ccgcaaaggc actgcgaccc atttgcgtca ccatcagatt gcgttcattc
    36361 atcgcctgca tggcaatcgc cgtagaggag agcgccagcg tcatgccgat caattccgcg
    36421 acctgccagc gcagtccaag caacatacag aacagcccca gcaggccgcc gcaaatcacc
    36481 atctgcaatg cgccgccgcc gaacaccgcc gcacgcagct tccacagcct ttgtggatcg
    36541 agttcgaggc cgataataaa cagcatcagc accacgccaa tctcggcaaa gtgcagaata
    36601 gattcggcat cggtcaccag tcgcagcccc cacgggccaa taatgcagcc agcgatcagg
    36661 tagccaagta ccgatcccag accaagacgt accgcaatgg gtacaatcag cgccgccgaa
    36721 ccgagataaa tcagcgcctg aatcagcgta tggctatcca tgatgtgcct cctgccattc
    36781 cagcagacgt tgcttatagt gacgcgcctg cccttcgagg gtttcgtcgt cacaaataaa
    36841 ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg cagtagattg ccgtcgcctg
    36901 tagcggctgt gacagcacat caaagcccgg atgcgcacca atttcaaaat ggctttcccc
    36961 gccgccggtc gtcaccgccc acagcaaatg tttgccatgc agcgccgtgc cgccgtgacc
    37021 gtaagcccag ccgtgggaga agactttatc gatccaaagt ttaagaagcg gaggaatgct
    37081 gtaccactgc atcgggtgct gccagacgat cagatcggcg cgagacagcg cctcctgctc
    37141 ggcggcaata tcgatattga agtcaggata gagttgataa agagagcgaa tttcgacgcc
    37201 ttccagcgtc cttgcctgtt caagcatccg tttattcgca tgggaatgat gcggatacgg
    37261 atgcgcataa attataagaa tcataggtag cccgcgtgat tacgcctttg ttttcccaca
    37321 aagtgtagtc atatattcac gcggcttata gtcaataata ttgattcaga aaagagagaa
    37381 atctgaacaa attaccccat tctctgcccg cggatcgtca tattgctggt ctgcgccagt
    37441 gacatccctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata
    37501 cccgccccca ttaacatcgt gttactgata ccgtaattat tgataaagat cggcagtgcc
    37561 caggtcgaaa caatagtgcc aatacggctt aaggacataa tcacgcccac ggcagaggcg
    37621 cggatatccg tcgggaagag ctcattagga tagagccact gcaaattacc cggcccgcca
    37681 gagaaaaagg catacaccgc aaaggccatc actaccagcc agatccccat atccgggatc
    37741 agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc
    37801 cgtccggcag tgtttaacca cagcatcggc ggaatacagc cgagcataaa gaacaggcta
    37861 atcaccacgt tccccagcgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt
    37921 tgcgggccaa aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca gatggcggca
    37981 acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc
    38041 ggctgctctt catcgaaagc caccggttcg ccaaacagtt tgatcatcat ctcttcgcac
    38101 tcttttactc gccctttgcg taacaaccag cggggagatt caggcagttc gaatcgacca
    38161 atcaaaatca acaaacaggg gatcgctgcg ctacccagca tccagcgcca accaccttcc
    38221 acatcataaa gccagtaacc gaccagatcg gcgcaggtcg cgccgacata ccacatggca
    38281 gcgataaagc tgatggaaaa cgcccgctga cgggtactgg agaactcagt gatcatcgaa
    38341 gtggcgatgg gataatctgc accgatgaca atgccgatga gtacccgcat cactaacagt
    38401 tcgacggggg atgagacaaa catcgtcgcc accgatatca cgccgatggc gatgatatcg
    38461 atgaggaaca ttttgcgccg tccgacttta tcagagatat agccaaacag cgatgtgcca
    38521 acgaacagcc cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc gtccagtttc
    38581 agcgccggag tcagttgctc cagcgccacg ccaatcatta ccagtacata accatccaga
    38641 aacggaccac cgcttcccca cagcaaaatg cggcggtgaa tagaagagaa tttaagatcg
    38701 tcaaagtttc tggacggttg catgggttat tcctgttttt ttgcaatgag agtcagtacg
    38761 gcgttacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg
    38821 gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag
    38881 acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg
    38941 gcacgctttc accagcagtt ccagcgcctg tttatcggca tcggccttca caataatgtg
    39001 cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg
    39061 agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact
    39121 ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg
    39181 aacaggtcac gcgccacgcc caccgccagt tccgggtagc cgctaaacat gcgtgggtta
    39241 tcaaggaacg ccggtagttt ctggtacata cgcatatcgc gcagcgggcc actctcaaga
    39301 tgctgacgat attccgccag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc
    39361 acggtttttg ctgcggcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa
    39421 ccgaggttca tacacattcc ggcggcatca ccggcaatca gtacgccgtc accgaccagc
    39481 tccggcagca tgttgatgcc tgcttccggc actacgtgag cggaatattc caccagcttg
    39541 ccgcctgcga tcagaggtgc aacggccgga tgttgtttga aatcttccag catttgcggc
    39601 accgattttt ttgcgtcatg cagatgatgg agaccacaaa ccagccccag cgacagggta
    39661 ttttcattgg tataaaggaa gccgccgccc atcaggccat cggtggggga acccgcaaat
    39721 aggcaagccg caccctgatt accctgcaac tgaaaacggt cttcaataac cgacttcggt
    39781 aactcgatca gttccttcac gccaaccgcc acatccgtcg gtttgacgcg ttttgccatc
    39841 cccagctttt cggcaaggat ggaattcacc ccgtcagcaa ggatcaccgt tttcgcttca
    39901 atcacatcgc catcggcttc tacaccgacg actttgccat cgcgctgtac gaggttatcg
    39961 acacgaatcc cggtaattaa ctgcgcgccc gcttcttcgg cctgctccat caaccaggca
    40021 tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gcgaggattc gtcaccgttg
    40081 cagtagtcca tagtcatcgc tgacttttcc gtcataaacg cgagtttttc atgggtgatc
    40141 aggcgttcta cgggggcgga ttcggcgaaa cctggaataa tgtgttccag actgtgggca
    40201 tagagacgcc cgccagtaac gttcttggca cctgcggaat tgccgcgctc gataactaac
    40261 acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag cccagcacca
    40321 acgatgatgg cgtcaaagat atcttcggac ataagagctc cctgtcagcg gcagggccaa
    40381 aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct
    40441 tcacggcgtc gccaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt
    40501 tgttgatggc gaaaatggtt tgcgacgcgt tagcgccaac catgtgctgg atctgcccgg
    40561 agatccccac cgccaggtac agttcaggtt tcagcatcag gttggagata ccgacatatc
    40621 gttcgtgctc catccatttt tcgttttccg ccaccggacg agaacaggcc aactccgcac
    40681 ctatcgcctt acaaagctgt tctgccagcg caatgttctc tttgcttcca atgccgcgac
    40741 cgacgctgac caccagacgg gctttgtcga gatcgacgct gttgctctgg cgcgcctggg
    40801 ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag
    40861 tgcgtgacgc gtctggctga gccgcatcaa acgtgccgct gctgatggtc agtaccgcat
    40921 acggcgtggc aatgcgttct tcgccaatcg ccaggccgcc gtaaaccatg tgtttcactg
    40981 tcgctttacc gtcctgtacg cagacggtgc tggcatcgtt agacaccgcc gctttaaggc
    41041 gataacccag ttttgccgcc agtaatttgc cgcgacgggt attcggcagc agcaccaggc
    41101 cgtctgcgcc gtgctggcga atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc
    41161 gctcgtccgg tttgccgttt aatttccaga catgattagc accgagctgg attgcctgtg
    41221 cgccgtcggc atcattgagg acaaaggtgt tgatttgatt agctaaagcc tgcgcaccgt
    41281 tcatcagttc cggcagacga gaaggggtat cgctgaatac ccagacttga gaaaacgtgt
    41341 tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaacg cgacgatctg
    41401 ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg gtgcggcaac
    41461 ctgttgttca gaccaggctt ccacggcgtt aaagccgata tccgccgctg accatacctg
    41521 gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat
    41581 atcagtggaa acagcaacaa ccgcaggcag cggaatgctt aaggtttctg tttcatcttc
    41641 cagttcgcgc tcaacggtga gggtatctgc cgtcagggag ataattttgc tgacgccgtt
    41701 aactgccgga atattgagga tttcgcccac cagcaaacca acctgctggg cataaaggtc
    41761 ggaagaacca tcgccacaga ggatcaaatc aaagccagct ttctgtgccg cggcagccag
    41821 tgcagtcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag
    41881 ttcatccggg ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc
    41941 gcccacactt aaggccgtca cctgcccctc tgctgcctgt tgctttagct ggcaagccgc
    42001 ttcaatagcg ttgagatcgt attggcttat tttggcatcg gctttactga agtctaacga
    42061 accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat
    42121 aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat
    42181 tacagatatc acgcccttta atttggaaat aaaactttaa tcaccaatat tgaaaatgtc
    42241 acacgcagat aaatccaact ttcaatattg ttaagtccct caccaatatt gaaaacatgg
    42301 cgtagcaaaa agaaattttc aatattgctt tatggatcac caatattgaa agtcagatgc
    42361 tattttttta ctcacaatca aactcatcac attgataaac aatgaatatt tacacaaaga
    42421 cgccctgttt ttacccgata aaaaactgtg actaacatca cagaacgcag ccaattgaat
    42481 accca
//
LOCUS       DARNVM010000018        40757 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.018, whole genome shotgun sequence.
ACCESSION   DARNVM010000018 DARNVM010000000
VERSION     DARNVM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.018"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            298..1830
                     /gene="pntA"
                     /locus_tag="SI621_000961"
     CDS             298..1830
                     /gene="pntA"
                     /locus_tag="SI621_000961"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEI4063704.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            1841..3229
                     /gene="pntB"
                     /locus_tag="SI621_000962"
     CDS             1841..3229
                     /gene="pntB"
                     /locus_tag="SI621_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEI4063705.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTSIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3254..4288)
                     /gene="tqsA"
                     /locus_tag="SI621_000963"
     CDS             complement(3254..4288)
                     /gene="tqsA"
                     /locus_tag="SI621_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HEI4063706.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            4700..5065
                     /gene="mdtJ"
                     /locus_tag="SI621_000964"
     CDS             4700..5065
                     /gene="mdtJ"
                     /locus_tag="SI621_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HEI4063707.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            5052..5381
                     /gene="mdtI"
                     /locus_tag="SI621_000965"
     CDS             5052..5381
                     /gene="mdtI"
                     /locus_tag="SI621_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HEI4063708.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(5420..6241)
                     /locus_tag="SI621_000966"
     CDS             complement(5420..6241)
                     /locus_tag="SI621_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEI4063709.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDRLDQLSQK"
     gene            complement(6341..6424)
                     /locus_tag="SI621_000967"
     CDS             complement(6341..6424)
                     /locus_tag="SI621_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063710.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(6517..6852)
                     /locus_tag="SI621_000968"
     CDS             complement(6517..6852)
                     /locus_tag="SI621_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HEI4063711.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(7249..8502)
                     /locus_tag="SI621_000969"
     CDS             complement(7249..8502)
                     /locus_tag="SI621_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063712.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            8609..9502
                     /locus_tag="SI621_000970"
     CDS             8609..9502
                     /locus_tag="SI621_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4063713.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            9637..10857
                     /gene="mlc"
                     /locus_tag="SI621_000971"
                     /gene_synonym="dgsA"
     CDS             9637..10857
                     /gene="mlc"
                     /locus_tag="SI621_000971"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HEI4063714.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            10982..11677
                     /gene="bioD"
                     /locus_tag="SI621_000972"
     CDS             10982..11677
                     /gene="bioD"
                     /locus_tag="SI621_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEI4063715.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(11630..12922)
                     /gene="clcB"
                     /locus_tag="SI621_000973"
     CDS             complement(11630..12922)
                     /gene="clcB"
                     /locus_tag="SI621_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HEI4063716.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTIQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13081..13695)
                     /gene="dmsD"
                     /locus_tag="SI621_000974"
     CDS             complement(13081..13695)
                     /gene="dmsD"
                     /locus_tag="SI621_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HEI4063717.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASFAPLVTAFQTQSDETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMQQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(13738..14592)
                     /locus_tag="SI621_000975"
     CDS             complement(13738..14592)
                     /locus_tag="SI621_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HEI4063718.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARITFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(14594..15211)
                     /gene="dmsB"
                     /locus_tag="SI621_000976"
     CDS             complement(14594..15211)
                     /gene="dmsB"
                     /locus_tag="SI621_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HEI4063719.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(15222..17645)
                     /locus_tag="SI621_000977"
     CDS             complement(15222..17645)
                     /locus_tag="SI621_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HEI4063720.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(17706..20132)
                     /locus_tag="SI621_000978"
     CDS             complement(17706..20132)
                     /locus_tag="SI621_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HEI4063721.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(20331..20636)
                     /locus_tag="SI621_000979"
     CDS             complement(20331..20636)
                     /locus_tag="SI621_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEI4063722.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            20744..21454
                     /locus_tag="SI621_000980"
     CDS             20744..21454
                     /locus_tag="SI621_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HEI4063723.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMHMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTFLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(21457..22017)
                     /gene="speG"
                     /locus_tag="SI621_000981"
     CDS             complement(21457..22017)
                     /gene="speG"
                     /locus_tag="SI621_000981"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HEI4063724.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(22052..22393)
                     /locus_tag="SI621_000982"
     CDS             complement(22052..22393)
                     /locus_tag="SI621_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HEI4063725.1"
                     /translation="MKITLSKRIGLLAFLLPCAMALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            22528..22854
                     /locus_tag="SI621_000983"
     CDS             22528..22854
                     /locus_tag="SI621_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEI4063726.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            23060..24274
                     /gene="rspA"
                     /locus_tag="SI621_000984"
     CDS             23060..24274
                     /gene="rspA"
                     /locus_tag="SI621_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HEI4063727.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            24286..25305
                     /locus_tag="SI621_000985"
     CDS             24286..25305
                     /locus_tag="SI621_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HEI4063728.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            25363..25473
                     /locus_tag="SI621_000986"
     CDS             25363..25473
                     /locus_tag="SI621_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEI4063729.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(25493..26773)
                     /locus_tag="SI621_000987"
     CDS             complement(25493..26773)
                     /locus_tag="SI621_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEI4063730.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLPDNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(26808..27059)
                     /locus_tag="SI621_000988"
     CDS             complement(26808..27059)
                     /locus_tag="SI621_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HEI4063731.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(27132..29603)
                     /locus_tag="SI621_000989"
     CDS             complement(27132..29603)
                     /locus_tag="SI621_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEI4063732.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQEKRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENVTVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPSAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYKGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSTNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHS
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(29697..29888)
                     /gene="ydfD"
                     /locus_tag="SI621_000990"
     CDS             complement(29697..29888)
                     /gene="ydfD"
                     /locus_tag="SI621_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HEI4063733.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(29885..30073)
                     /gene="dicB"
                     /locus_tag="SI621_000991"
                     /gene_synonym="ftsT"
     CDS             complement(29885..30073)
                     /gene="dicB"
                     /locus_tag="SI621_000991"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HEI4063734.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNTVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            30489..30776
                     /locus_tag="SI621_000992"
     CDS             30489..30776
                     /locus_tag="SI621_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063735.1"
                     /translation="MRFRVVCLLLTTSGEVVSFLFRITKKEIYMTKEEFVSYIFDKTV
                     EMYAATYGSCNPLNKPEGKDDFDKIYRFLEDRYIKRLEDAGIKSPVKSPLS"
     gene            complement(30745..31122)
                     /locus_tag="SI621_000993"
     CDS             complement(30745..31122)
                     /locus_tag="SI621_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063736.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPGVLSGEICTEISSELLPVIREAICRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
     gene            complement(31122..31274)
                     /locus_tag="SI621_000994"
     CDS             complement(31122..31274)
                     /locus_tag="SI621_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HEI4063737.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDDTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            complement(31467..31874)
                     /locus_tag="SI621_000995"
     CDS             complement(31467..31874)
                     /locus_tag="SI621_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4063738.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            31952..32179
                     /locus_tag="SI621_000996"
     CDS             31952..32179
                     /locus_tag="SI621_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEI4063739.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            32163..32684
                     /locus_tag="SI621_000997"
     CDS             32163..32684
                     /locus_tag="SI621_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063740.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            32665..33630
                     /locus_tag="SI621_000998"
     CDS             32665..33630
                     /locus_tag="SI621_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063741.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRHPGAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            33671..34072
                     /locus_tag="SI621_000999"
     CDS             33671..34072
                     /locus_tag="SI621_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEI4063742.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(34291..36078)
                     /locus_tag="SI621_001000"
     CDS             complement(34291..36078)
                     /locus_tag="SI621_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HEI4063743.1"
                     /translation="MDDINISLDELNTIDLSPLIQSKLPVTCFDITKYLSSLGDGRAE
                     IRLLTHICGFHFRPENPQVPFGPCFQSSQGRSAIPDDINGLSLEVLSQFCPTIELPEL
                     QARIADTLWVRKIGGIRFPLLAVRAYYASSIAIMTSQGTWVSALERLERALRLCCFFR
                     KNTDFRDEFDQLSAHLLAEYERTREQGDSPYPLRLLQLDIDCKVSEPSFIAQELLFLT
                     KNYLAQKLFSFAVDACKTAIPIANRCCDRDTQFEFWRLLADTHLEESKFQDGGMISAA
                     CMQNAIEALANIPGTRIERLALYEEMRDYQIESRHQMSILQSPPQDISEIVHQAKSRV
                     VGRDLFDMVFRLAMLVCRPTSIERLKAQAIEQKANSIAWMFGSTHIDHEGMTLARIPA
                     GLGIDDANGAVIWPIMMTRMRIDHELAVAGQIIPATDEITMKYPISEAFFRDMFINHP
                     FIPFGHEEFFIKGMVCGFNGDFMTACHVLIPQIENSLRYVAKIKGEEPSQLHGDGSQE
                     RNGLKGLLDNPLIIEAFGVDIIGNLQALLVDKIYGELRNQLAHGYVPAGYYNQPPCIF
                     AWWLVLHILMNPTARYWQATYGQESEAQT"
     gene            36752..36907
                     /gene="hokD"
                     /locus_tag="SI621_001001"
     CDS             36752..36907
                     /gene="hokD"
                     /locus_tag="SI621_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HEI4063744.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            37124..37375
                     /locus_tag="SI621_001002"
     CDS             37124..37375
                     /locus_tag="SI621_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063745.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            37442..37720
                     /locus_tag="SI621_001003"
     CDS             37442..37720
                     /locus_tag="SI621_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063746.1"
                     /translation="MARNVKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            37722..38771
                     /locus_tag="SI621_001004"
     CDS             37722..38771
                     /locus_tag="SI621_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HEI4063747.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKPPQEKTVVSINVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALATGVLA"
     gene            38785..39537
                     /locus_tag="SI621_001005"
     CDS             38785..39537
                     /locus_tag="SI621_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEI4063748.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(39712..39914)
                     /gene="cspF"
                     /locus_tag="SI621_001006"
                     /pseudo
     CDS             complement(39712..39914)
                     /gene="cspF"
                     /locus_tag="SI621_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            40213..40428
                     /gene="cspB"
                     /locus_tag="SI621_001007"
     CDS             40213..40428
                     /gene="cspB"
                     /locus_tag="SI621_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HEI4063749.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT        10319 a         9542 c        10222 g        10674 t
ORIGIN      
        1 tagtgattcg tgccggggcg atgtctcgtt ttacccgacc gccgaagaca attatcagtc
       61 tttatccggc gttctaaggt gtttatccca ctatcacggc tgaatcgtta atattttgcg
      121 agttcacgcc gaaatactga tttttggcgc tagatcacag gcataatttt cagtacgtta
      181 tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac atgagcggct
      241 tgtgtgactc ctgacacagg caaaccatca tcaataaaac cgatggaagg gaatatcatg
      301 cgaattggca taccaagaga acggttaacc aatgaaaccc gtgttgcagc aacgccaaaa
      361 acagtggaac agctgctgaa actgggtttt accgtcgcgg tagagagcgg cgcgggtcaa
      421 ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt agaagggaat
      481 agcgtctggc agtcagagat cattctgaag gtcaatgcgc cgttagatga tgaaattgcg
      541 ttactgaatc ctgggacaac gctggtgagt tttatctggc ctgcgcagaa tccggaatta
      601 atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga tggactctgt gccgcgtatc
      661 tcacgcgcac aatcgctgga cgcactaagc tcgatggcga acatcgccgg ttatcgcgcc
      721 attgttgaag cggcacatga atttgggcgc ttctttaccg ggcaaattac tgcggccggg
      781 aaagtgccac cggcaaaagt gatggtgatt ggtgcgggtg ttgcaggtct ggccgccatt
      841 ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg acacccgccc ggaagtgaaa
      901 gaacaagttc aaagtatggg cgcggaattc ctcgagctgg attttaaaga ggaagcgggc
      961 agcggcgatg gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga aatggaactc
     1021 tttgccgccc aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat tccaggcaaa
     1081 ccagcgccga agctaattac ccgtgaaatg gttgactcca tgaaggcggg cagtgtgatt
     1141 gtcgacctgg cagcccagaa cggcggtaac tgtgaataca ccgtgccggg tgaaatcttc
     1201 actacggaaa atggtgtcaa agtgattggt tacaccgatc ttccgggccg tctgccgacg
     1261 caatcctcac agctttacgg tactaacctc gttaatctgc tgaaactgtt gtgcaaagag
     1321 aaagacggca atatcactgt tgattttgat gatgtggtga ttcgcggcgt gaccgtgatc
     1381 cgtgcgggcg aaattacctg gccggcaccg ccgattcagg tatcagctca gccgcaggcg
     1441 gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc accgtggcgt
     1501 aaatacgcgt tgatggcgct ggcaatcatt cttttcggct ggttggcaag cgttgcgccg
     1561 aaagagttcc ttgggcactt cactgtgttc gcgctggcct gcgttgtcgg ttattacgtg
     1621 gtgtggaatg tttcgcacgc gctgcataca ccgttgatgt cggtcaccaa cgcgatttca
     1681 gggattattg ttgtcggagc actgttgcag attggccagg gcggctgggt tagcttcctt
     1741 agttttatcg cggtgcttat agccagcatt aatattttcg gtggcttcac cgtgactcag
     1801 cgcatgctga aaatgttccg caaaaattaa ggggtaacat atgtctggag gattagttac
     1861 agctgcatac attgttgccg cgatcctgtt tatcttcagt ctggccgggc tttcgaaaca
     1921 tgaaacgtct cgccagggta acaacttcgg tatcgccggg atggcgattg cgttaatcgc
     1981 aaccattttt ggaccggata cgggtaatgt tggctggatc ttgctggcga tggtcattgg
     2041 tggggcaatt ggtatccggc tggcgaagaa agttgaaatg accgaaatgc cagaactggt
     2101 ggcgatcctg catagcttcg tgggtctggc ggcagtgctg gttggcttta acagctatct
     2161 gcatcatgac gcgggaatgg caccgattct ggtcaatatt cacctgacgg aagtgttcct
     2221 cggtatcttc atcggggcgg taacgttcac ggggtcggtg gtggcgttcg gcaaactgtg
     2281 tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga acctggcggc
     2341 tctggtcgtt tccttcctgc tgctgattgt atttgttcgc acggacagcg tcggcctgca
     2401 agtgctggca ttgctgataa tgacctccat tgcgctggta ttcggctggc atttagtcgc
     2461 ctccatcggt ggtgcagata tgccagtggt ggtgtcgatg ctgaactcgt actccggctg
     2521 ggcggctgcg gctgcgggct ttatgctcag caacgacctg ctgattgtga ccggtgcgct
     2581 ggtcggttct tcgggcgcta tcctttctta cattatgtgt aaggcgatga accgttcctt
     2641 tatcagcgtt attgcgggtg gtttcggcac cgacggctct tctactggcg atgatcagga
     2701 agtgggcgag caccgcgaaa tcaccgcaga agagacagcg gaactgctga aaaactccca
     2761 ttcagtgatc attactccgg ggtacggcat ggcagtcgcg caggcgcaat atcctgtcgc
     2821 tgaaattacc gagaaactgc gcgctcgtgg tatcaacgtg cgtttcggta tccacccggt
     2881 tgcggggcgt ttgcctggac atatgaacgt attgctggct gaagcaaaag taccgtatga
     2941 catcgtgctg gaaatggacg agatcaacga tgactttgct gataccgata ccgtactggt
     3001 gattggtgct aacgatacgg ttaacccggc ggcgcaggat gatccgaaga gtccgattgc
     3061 tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg attgtcttta aacgttcgat
     3121 gaacactggc tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca cccacatgct
     3181 gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac cctgacggcc
     3241 tctgcggagg ccgtcactct ttattgagat cgcttaacag aacggcgatg ctttgacctc
     3301 ccgctgtttg ttcaagcgca attttgacaa taattgtcaa cggcacggaa agcagcatac
     3361 ccaccggtcc taacaaccat ccccaaaaaa tcaacgacaa aaataccacc aatgtggaaa
     3421 gccccagccc acgccccatg atgcgtggct ccagaatatt gccgaagacc agattaatca
     3481 gcagatatcc cgccagcacc agcaacgctt cgtagaagcc attaaacacc agtacctgag
     3541 cgataggggg aattgccgcg agaactgaac caatattagg gatgtaatta agcgcaaagg
     3601 ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc gcccaggcga
     3661 ccaggccggt gatgatgctg atggctgttt tcagcaccag ataatgagaa acactgtcaa
     3721 tcgcacgttg aatcgccgcc atcccttcaa ccggacgcgc catcatttgc tgaaattttc
     3781 cgggcaattg tggcacttcg agcagcataa acagcaccgt cagcagcaat aaaaatattg
     3841 atgacatggc attagataac tgcgtcaata agttggtgag caacgtcatc gccgcgttcg
     3901 gatcaatgta atgcgccagt tggtcaactg agacatcaat ccctacgcgt tgcaacaacg
     3961 gttcaatcgc ttgcagcggc gtcataatag agttgcgata ttgcggtaac gtccgcgtca
     4021 actcgttgag tgcggaaccc agataagcta acagcaacac catcgccatc acgatgatgg
     4081 tcatcaaaat cgacaccgcc agtacacgcg gcactcgcca gcggaccatg tgttgcacca
     4141 gcgggttaag aataacagca ataaataatg cgagaataaa cggcacgatg atctcggcgg
     4201 caaaacggat accgcagaga ataatgacca gcattcccaa cataatgacg atttttaggc
     4261 cattgagcgt gatgatcggc tttgccatgc ttgtccccgt tttttcttcc tttgctaata
     4321 ataatgtctt tttcgctccg tcatcataac gaaaatcaaa ccgctccggt aaagaagtga
     4381 aaatattttg agttaattct taagctatga tacaaatcag gcgtgttcaa ctaccgagga
     4441 caattatcat ccgcgatgac gagaagcaac actgcggata attgtaatat tatggacaat
     4501 ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac tatcttgctc
     4561 gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcttaa gcttacgcta
     4621 tgcgcggggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat taaaataatt
     4681 ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg ctattgctac
     4741 agaaattacc ggtacgctgt ctatgaaatg ggcgagcgtc agtgagggaa atggcggctt
     4801 tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg ccgttaaaaa
     4861 gatcgcctta ggcgtggctt atgcgctgtg ggaaggtatc ggtattttat ttattacctt
     4921 gtttagtgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg ggttaaccac
     4981 cctggtggcc gggattgtat tgatcaaatc aggtacccgt aaagcgcgta aacctgaact
     5041 ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ctggctggca ttggcaatcg
     5101 tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt cgcaaaatat
     5161 ttggcttgct ctccctggcg gcggtgctgg ctgcctttag tgcgctttct caggccgtta
     5221 aagggatcga cttgtctgtc gcttatgcat tgtggggcgg gtttggtatt gccgccacgt
     5281 tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg attggcctgg
     5341 tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaacacgc tgcccgcgct
     5401 gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcctgtcg cgaaaaccgg
     5461 taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga gccgaacttt
     5521 gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa ccgctgtcac
     5581 ccggcaaggt atcgcactga tgcgacatca ccgacgtttg cgcccagcca gtcacttcac
     5641 agttttgatg actgtacaac gtatcgagat gatcttcagg gtatcccgcc tgagtcactt
     5701 tacgacctgc cgcttttaat gcggcggtaa gcgcggcttt atctccctca aataacggca
     5761 atggtgtaat gccagaaggc ggattacgta gcacaatcaa accgaagtcc cacggcgcag
     5821 ctgcgggagg aacaatccaa ccatccccat ctgcttttaa ccgctttccc agtgtcggat
     5881 caacgcggcc ttctatgtcg tggatctcat agcgccaaag acctttattt gacacgaaac
     5941 gcagcgctac cgctttatcg gctttaccct ttggcggcgt caataaacag tgtcctgccg
     6001 ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg gccgtttcca
     6061 gttggccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca cgatcatcat
     6121 gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct ggtttatctg
     6181 caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg gttgtacgca
     6241 tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa tactatagac
     6301 gggacgggat gaaagtggga gtaaaatcag ataactacga tcaggaaaga taaaaacggg
     6361 cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga taacggccca
     6421 ccatactacc ctccgctaaa ggcggtgcta aacgttatcc gcttagcacc gtaattttca
     6481 ggcgcgaggg ggcgcgccag cattactatt gataacttac gctgcgggtt gtgcagcagg
     6541 ttttgccggt tgctgatggc tgtgtttctt ggcgtgtttc ttcgctgcct gcgctttttg
     6601 ttcaggagct ttctgttcag ctttggcatt tttatgatgc tttttagccg cctgcgcttt
     6661 ctgggcaggt gctgctttat gctgtttttt atgatgtgtg gttttcgcag gcgctgcttt
     6721 ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg cggcagaaga
     6781 cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca taccctcaat
     6841 ttgttttttc atttaacccc actgcggggc cgttgaaata actatatccc tgggaatggc
     6901 agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct gaataaatta
     6961 cagcgttgat aataacgtat tgtgacttaa gggaaattta gctaccaata atagtagtct
     7021 tgatcgggtt aactatttta ctacttacaa agcggtagaa taactgcgca tcagtaataa
     7081 atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat gacactagat
     7141 gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac gttggtttga
     7201 gcaggcaata agtccggatg ggtatttacc gcagtccgga cttatttttc aggcgtgcag
     7261 acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag caataaatgc
     7321 gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc cggcaatact
     7381 cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag cgcgttttgc
     7441 gcgggggccg atccagctgc tggctactga gtgggctgcg aagaatccgg ctgagaagag
     7501 taacattccg gcaaagatca gccacagcga gctgaataag gtcatcaata aaccaaacag
     7561 cataaccccc gtcgaaaaca acatcaccgg accgcgccca tagcgggtgg tcatggttcc
     7621 ggcttttggt gagctccatg taccggtcaa ataagccagc gataataagc caaccacggc
     7681 ctggctgaca tgccaggggg agagcatcaa ccgatagccg atgtaattaa acagcgtgac
     7741 gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc ggtcacgcca
     7801 gtgcagacga aagttgataa acaacgtctt aggtcgcagc gaagtcgggc gaaaatggcg
     7861 tgattcaggg aggattttcc agaacattaa cgccgaggcc agcgcgaaac aaccgattgc
     7921 cgccagcgca attcgccagt tgaaaaagtc cgtgaagaca ccgctaatta agcgtccgct
     7981 catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca cgaaactggg
     8041 gtggatttcc tcgctaagat aagtcatgcc aacagctgct acgccactta acgaaagccc
     8101 aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgtcg aaagtaacgt
     8161 acaaatggag gccaacagta gcgccgtgac catcactggt ttgcgaccaa tggcatcgga
     8221 taacgggcca gtaaacagca aaccgatagc caacatcgcc gtggaaatgg acagtgaaat
     8281 actactgttc gcgggcgtta agccaaactc ctgtgaaagc accggaagga taggttgcac
     8341 acaatagaga agtgcgaagg ttgccagtcc ggcagagaac agcgccaggg tgacgcgcat
     8401 aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt gcttgtcagt
     8461 gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc cttgctaaat
     8521 atgcctgtgg atcaggctta tgaatagatt aggaaaatcg aattgttctg tctaatatat
     8581 taataatctc atataagatg ttttaaatat gaatattgaa cttcgtcatc tgcgttactt
     8641 tgttgcggtt gcagaagagc tgcatttcgg gcgcgccgct gcccgcctga atatttcgca
     8701 accaccgcta agtcaacaga ttcaggcgct ggagcaacaa attggtgccc gactgctggc
     8761 acggaccaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg tagatagtcg
     8821 gcaaatcttg tctatggtgg atgatgctgc cgctcgcgct gaaaggctgc atcagggcga
     8881 agcgggggag ttgcgcattg gttttacttc gtccgctcct tttattcggg cagtgtccga
     8941 tacgttatcg ctgtttcgcc gtgattatcc cgatgtccat ttacaaaccc gcgaaatgaa
     9001 cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat tgctgcgtaa
     9061 cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc ttatggcgat
     9121 gatcccgcac gatcatcccc tggcaaataa cccgagtgta acgctggctg aactggcgaa
     9181 agaacccttt gtcttttttg atccgcacgt agggacaggg ctgtatgacg atattctcgg
     9241 gctgatgcga cgttacaatt tgacgcccgt catcactcag gaggtgggtg aggcaatgac
     9301 catcatcggt ctggtttccg ctggtctggg tgtttcaatt ttgcctgcgt catttaaacg
     9361 tgttcagctc aacgaaatgc gctgggtgcc gattgctgaa gaggatgctg tttctgaaat
     9421 gtggttggtc tggccgaaac accatgaaca aagtccggct gcgcgtaact ttcgtattca
     9481 tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa agcccgaaaa atgtgctgtt
     9541 aatcacatgc ctaagtaaaa atttgacgac acgtattgaa gtgcttcacc atagcctaca
     9601 gattatttcg gagcgcgaaa atatagggag tatgcggtgg ttgctgaaaa tcagcctggg
     9661 cacattgatc aaataaagca gaccaacgcg ggcgcggttt atcgcctgat tgatcagctt
     9721 ggtccagtct cgcgtatcga tctttcccgt ctggcgcagc tggctcctgc cagtatcact
     9781 aaaattgtcc gtgagatgct cgaagcacac ctggtgcaag agctggaaat caaagaagcg
     9841 gggaaccgtg gccgtccggc agtggggctg gtggttgaaa ctgaagcctg gcactatctt
     9901 tctctgcgca ttagtcgcgg ggagattttc cttgctttgc gcgatctgag cagcaaactg
     9961 gtagtggagg agtcgcagga actggcgtta aaagatgact cgccattgct ggatcgtatc
    10021 atttcccata tcgatcagtt ttttatccgc caccagaaaa aacttgagcg tctgacttcg
    10081 attgccatta ccttgccggg aattattgat acggaaaatg gtattgtaca tcgcatgccg
    10141 ttctacgagg atgtaaaaga gatgccgctc ggcgaggcgc tggagcagca taccggcgta
    10201 ccggtttata tccagcatga tatcagcgca tggacgatgg cagaggcctt gtttggtgcc
    10261 tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc acaacgtggg ggcgggcgtc
    10321 attaccgatg gtcatctgct acacgcaggc agcagtagcc tcgtggaaat aggccacaca
    10381 caggtcgacc cgtatgggaa acgctgttat tgcgggaatc acggttgcct cgaaaccatc
    10441 gccagtgtgg acagtattct tgagctggca cagctgcgtc ttaatcaatc catgagctcg
    10501 atgttacatg gacagccgtt aaccgtggat tcattgtgtc aggcggcatt gcgcggcgat
    10561 ctgctggcaa aagacatcat taccggggtg ggcgcgcatg tcgggcgcat tcttgccatc
    10621 atggtgaatt tatttaaccc acaaaaaata ctgattggct caccgttaag taaagcggca
    10681 gatatcctct tcccggtcat ctcagatagc atccgtcagc aggcccttcc tgcgtatagt
    10741 cagcatatta gcgttgagag tactcagttt tctaaccagg gtacgatggc aggggctgcg
    10801 ctggtaaaag acgcgatgta taacggttct ttgttgattc gtctgttgca gggttaacat
    10861 tttttaactg ttctaccaaa atttgcgcta tctcaatttg ggccaggaaa gcataactta
    10921 gactttcaag gttaattatt ttcctggttt atatttgtga agcataacgg tggagttagt
    10981 gatgctgaag cgtttcttta ttaccggtac agacacttct gtagggaaaa cggtggtttc
    11041 ccgcgcattg ctacaagcgt tagcctccca gggaaaaacg gttgcgggat ataaacccgt
    11101 agcgaagggg agcaaagaga cacccgaagg gctgcgtaat aaagatgccc tggtgttgca
    11161 gagtgtttca accatcgaac tgccttatga agcagttaat cctatcgcgt taagcgaaga
    11221 agaaagtagc gtggcgcaca gttgcccaat caattacacc ctcatttcaa acggcctggc
    11281 aaacctgacc gataaagtcg atcatgtggt ggtagaaggg actggcggct ggcgcagtct
    11341 gatgaatgat ttgcgtccgc tctctgaatg ggtggtgcag gaacaactgc cggtattgat
    11401 ggttgtcggt attcaggaag gttgcattaa ccatgcacta ctaacagctc aggcgattgc
    11461 caacgacggg ctgccgctca ttggctgggt ggctaaccga atcaatccag gactggcgca
    11521 ttatgcggaa atcattgatg tgctgggtaa aaaacttccg gcaccgctca ttggtgaact
    11581 gccttatctg ccgcgcgccg aacagcgtga actggggcaa tacatccgct tagctatgct
    11641 gcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt ccgcgaaatt accgacgcaa
    11701 ttacgcaggc aatcaataaa ccggggagta gctgatattc cccggtcatt tcacatatca
    11761 tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa cagtgtcgcc atcccggtca
    11821 atccgagtaa aagcgtaatt tcttcgccat cagggaacca taatcccagg ctacgaccat
    11881 acaacatgcc aatggcaaga ccgataaata gcgtcggtgt aaagacacca ccgggcgcgc
    11941 cagaaccact actcgccagc acggcaaaca gtttacagag gaagatcccg gcaatgatca
    12001 ttaacagtgg cggagcggtt aaaaaggatt gtacggtgct atagccgttg ccccacactg
    12061 caggtgtgaa cagggaaagc agacccacga tcaacccgcc cagtgccagt tgccagggcg
    12121 gcgcaagttt gagactcaca aatccacgat gacaggcgtt cattaacgtt aacaacagtg
    12181 gtccgcacag acctgccagc acacctgtac tgataatcag cgcatagtca cgagcctgaa
    12241 tcgtcactga gagttgtacg ctgtagagta acgcgtcgct atgattaatc agattgctaa
    12301 ccagcaacgc cacgacagcg gaaataatca ccgggccgag agaggccaac atcatagtgc
    12361 caaacagcac ttcggctata aataaactgc cagcaagcgg ggcacgatag gccgcagcca
    12421 ttcccgccgc ggccccacag gcgatccata atttccactc ctggcgtggc gtaaaacgtt
    12481 gggcaaaaca ggaggcggca agggcagcta aaagaatcat cgcaccttcg cgaccaattg
    12541 cactgccgct ggttactacc agcagagagg caagcgattt aaccaggctt gctgcgtaat
    12601 caaactgccc atcggtttgc aacgcttcca tgtaatcggt cggcgcatga gggcgttgct
    12661 gggtaaattt ctgccagccc atcagcaaca aacccgccgc cagtccgccg agcgccggag
    12721 ttagcagccg tcgccagggg gaaagatttg tcgctgcatt gaccagactg ccggagtcat
    12781 tattgaggaa caaccactcc agtagcagca ttgcatgacg aaacccggca acggcaaagg
    12841 ccgcaagaat accgacgact gttgcgataa gcagacggcg gaacatagtg cgcagatccg
    12901 gatagatgtg tagatgatgc atgagcagaa atgggcaatg aaacacgggc gactattttg
    12961 cggcaaaaga ggccagctgc aaaagcagtg cgtgctttaa gagataaaaa aacgtgacgc
    13021 tgtaacctat tattgccgaa ttgcgcgtaa tcgtcaccat ccggcaatat tacggtgatc
    13081 ctatcgaaag agtggtttaa ccgctacagg aattaacagt tgtgattgcc attgcgccag
    13141 cgttaagcga gccagttcac ccagtgcacg gtagaaaggg tgttctgctt tttcgataaa
    13201 aacgtcaaga aatcgtgttg accacggaaa aaggtgccat gccagcaatt cttcgcattc
    13261 tgtctggcga ccattctccg ccaaccacgc agccatcaac agcagtgaac caaaatgatc
    13321 ttccggttcg ttttgctgca tttcaaactg aatgcctttc tcgcgcatcc actgacgaag
    13381 tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc agccagaccg atccccacgg
    13441 cggtgacggc agtgcccacg ggccgacaaa caaacgctgc caggcctggg cgtgagtctc
    13501 gtcactctgt gtctgaaaag ccgtcaccag cggcgcgaat gacgcttctg gtagaggcca
    13561 ctgagtttcc cagccgtcac gggtgagtac cgcaaccagg ggggcggctt ccgcactctc
    13621 tggagcgtaa taaaacagcg cgcccaacac gcgcgccgcg acagaaaaat tatcttgctg
    13681 tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt tgccccgcac caatttgtta
    13741 acctgcaata gccattccta ccgtcatgtg caggccataa aagagcacac gcccgatcat
    13801 ctcaccgcca agtatgagga ttagccccag aacaagtccg gcaacgtgag gttcacggcg
    13861 acgaatgagc ggacaaagcc agcatcctag acctgcgcat aacaatacca cacgccagac
    13921 ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt tgtacggatg agtgaataga
    13981 cgccagagac aaaccttgca gcacaatcac cccggcacaa gcgatcagcg ccagaacgct
    14041 aatgatggca aagggtgtgg tattaaatgt aatgcgggct gcccgcagaa ttgtggcagc
    14101 aagtattgga ccgctcaaca atactgtcag gaagaaagcc agcgtagtgt aaccgttatg
    14161 ccaggttggc actgtgtcga tttgatacac gcaggtcatc atccagacga aaatgacgcc
    14221 gagcgccatg ctgacaagta accagagttt ccccaacgct tgcggcattt taccgatgac
    14281 cgccaccagc caccataatc cgccaacggc aaaaaatatg gagccggcag caatctcgtt
    14341 actcagacca gaagctccga ttcgattcag cgagttaaac gcccgcagtg gggatcccag
    14401 gtgcatgaca gaggcaataa acccgatgcc catcaacagc cagaggaaaa acatgccacg
    14461 aacaatgtgc tgacggtctg cgtcattttt ggcggcgaac cagccaattc cgctaacaat
    14521 cagcgcgcct actacgcatt gacccaatac ggtaaagata accagtggcc attcatgcca
    14581 tccatttccc attttacact tcctccggat tagccagata accagtggta tcgccggttg
    14641 ggcggctgtt ggcgttaggt ttgataacaa tattgggttt tgtgaaatgc gcgcgcggca
    14701 gtggcgcgac ggcagccaga gtgccgtgct tctggcgcag ctcttcaatt ggaccgaact
    14761 caagtgcacg cagcgggcag gattcgacac atatgggttg tttcccctcg gcgacgcgcg
    14821 aataacaacc atcgcacttc gtcatgtgcc ctttttcagc attgtactgt ggcgcgccgt
    14881 acggacaagc catgtggcag tagcggcagc cgatacaaac atcttcatca accaccacaa
    14941 agccatcttc acgcttatgc attgcgccgc tggggcagac ttttgtacat gcggggtcat
    15001 cgcaatggtt acaggagatg gagagataat aagcgaacac gttctggtgc cagacgccat
    15061 tatcttcctg ccagtcgccg ccagcatatt cataaatacg gcggaaactg acttccgggc
    15121 caagatcttt aaaatctttg cacgccagtt cgcaggtttt acagccagta cagcggctgg
    15181 aatcgataaa aaatccatat tgtgtggtca tgggctactc cttaaacctt ttcgatctgg
    15241 acaagattgc tgtgcgacgg gtttcccttt gccagcggtg aagggcggtg agaggtcaga
    15301 atattgatac tgccgccgtg atcgacccgg tcaccaaaca tatccgcttt aagccacgca
    15361 ccttgcccga tggcggtaac gccaggcaga atacgcggag tcacttttgc ggcaatcagc
    15421 atttctccat tattgttaaa tacccgcacg gtatcgccat gacggatacc gcgtgcctga
    15481 gcatcaatgg ggttgatcca cacctcttgt gggcaggcct gctgtaacac atcaatattg
    15541 ccgtagctgg agtgggtacg cgctttgtag tggaagcccg ttaactgcag tggataggtt
    15601 ttccgcaggg gatcgtccca gccatcaaaa cctggggtat acgcaggaag gggatgaata
    15661 atttcatctt ttttcaattc ccaggtatct gcaatcttcg ccagtcgttc agaataaatt
    15721 tcgattttcc ccgaaggtgt tttcaacggg tttgcctgtg gatcttcacg gaatgcgcgg
    15781 aaagcgacgt agtgttcttc cgggcatttt ttcttaaaga tcccggtcgt tttcatctcc
    15841 tcgtagtcgg gcatctcagg gttacgttcc ttcgttttcg catggagata tttgatccat
    15901 tcatgctgac tgcggccttc agtaaaggtt tgataaacgt ctggtcctaa gcgtttggcg
    15961 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    16021 gcgaggatca catagcccat attcccggca gattcgtgag agataaggtc ttcttgctct
    16081 gttggcatca ggtcgggcaa caggatatcg caatacttag ccgaggcagt catgaagtgg
    16141 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    16201 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    16261 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttc cgtaccatgg
    16321 tcgatggcat ctgtccatgt aaagacggaa atctgcgttt taacaggatt ctcgagcatc
    16381 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    16441 atgccgacgt taccggtgag aacggaaagc atggcaatag cgcgggacgt ttgctcgccg
    16501 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    16561 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    16621 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    16681 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    16741 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgctcca
    16801 tcggtgccag ggcgaatggg cagccattca tcttcacgtc cggcagctgt gtcgttataa
    16861 cgtggatcga tgacgatcat gcgtgcattt gaacgttcgc gggcttgctc gacgtagtaa
    16921 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    16981 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    17041 gcggcactga tttgagcggt actgtagctg ccatagcgac tgagaaaacc accgcaagag
    17101 ttcatcagac ggtacgggac gtttgagttg gtgatgtttc cgccatctac gcctgttcca
    17161 tagagaacat gtacagcctc attgccgtaa tctttaagga tccggcgaag attatcactg
    17221 atggtatcca gggcttcatc ccaacttatc cgttcaaatt taccttcacc gcgcttgccg
    17281 acgcgcttca tgggatattt caacctatca ggatgattca tccgtcggcg gatagagcgc
    17341 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    17401 tccacccagt acacggtgtc atctttcaca tgcaaacgta acagacagcg gctcccgcag
    17461 ttaacggtgc aggaactcca gaccgctttc tcttctaccg gtgtctctgc cgctcggacc
    17521 atttgggaaa atggcagagt gaaagcactg cttgccagcg caagactgcc aagtgcggag
    17581 gttttcatca gacttctacg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    17641 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    17701 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    17761 ggtgaagggc gcagagtggt tagcgtattc acacagccgc catggtcgat tttatcgcca
    17821 gacatattgg cctcgtgcca ggctccctgg cccatagcgc taaccccagg gagaatgcgt
    17881 ggtgtcactt tggctggtag ccgaacttcg ccacgatggt tatacacccg caccatatcg
    17941 ccgttggcaa tcccacgttt ctgtgcgtct atagggttga tccacacctc ctgacggcag
    18001 gcagccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    18061 ccaaacagtt gcagtgggaa ggtactacgt tcaggggagt cccagccttc aaaggttgag
    18121 gcataaaccg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    18181 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    18241 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatgac cattgggatc tttacgctta
    18301 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    18361 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    18421 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttgcgttcg
    18481 aatttttcgc tggtgactgg ctgaaggaaa atgagatatc ccatgttacc ggcgtagtcg
    18541 ttaggaataa tatcttcctg ctcaacggtc atcaggtctg gcagcagaat gtcggcatat
    18601 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    18661 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taaccagggt atttcccgcg
    18721 tagttccaga tgaacttaat gggcacatcc agtttatctt tgccgcggac gccgtcgcgg
    18781 attgccgtca tttgcgggcc atgatcgata gcatccgtcc agctgaagca ggagattgac
    18841 gttttgaccg gattatccag caccggtagg cgttctatgg taatggtata ggtcgattca
    18901 cgcgcgccac tattcccgcc gctgatgccg acattgcccg tcaaaatagg caacatagca
    18961 atagcgcgtg cggtcagctc accgtttgcc tggcgttgcg gcccccagcc ctggcagata
    19021 taagcgggtt ttgctgtgcc aatttcacgc gccagtttga tgatacggtc caccgggata
    19081 ccggtaattt gcgaagccca ctgcggcgtt ttcgctgttt tatcgtcacc ttcaccaaga
    19141 atataggctt tatagtgacc attttttggt gcatctgcgg gtaaggtttt ttcgtcatag
    19201 ccgacgcagt atttatcaag aaaaggttga tcaacgagat tttcgttaat caatacccag
    19261 gcaatacccg caaccagcgc ggcatcggtg cccgggcgaa taggtagcca ttcgtcttca
    19321 cgaccagcag ctgtatcggt atatcgcgga tcgataacaa tcatttttgc gttcgatttc
    19381 tcgcgcgctt tttcaagaag ataagtgatg ccaccgccgc tcatgcgggt ttctgccggg
    19441 ttgttaccaa acatcacgac cagcttgctg ttttcaatat ccgtggtgct gttgccatca
    19501 ttactgccgt aggtgtaggg catggcacag gaaatttgcg cggtgctgta ggagccatac
    19561 tggttgagtg aaccgccgta gcagttcatc aggcgtttga ccgccgaggc ggatggcgaa
    19621 gagcgggtca tattgccgcc aacgatccct gaagagtact gaatatatac agcctcattg
    19681 ccatattgtt cgacggtttt tttcaggcta ctggcgatag tatccagggc ttcatcccag
    19741 ctaatccgtt cgaatttgcc ttcgccgcgt ttgcccacgc gtttcattgg gtaattcaag
    19801 cgatcgggat gattaatacg ccgacggatg gagcgaccgc gcaaacaggc acgtacttga
    19861 tgattgccat actcgtcgtt gccggtgttg tcagtttcca cccaggtcac ttcattatct
    19921 ttaacatgca gacgaagtgc acagcggcta ccacagttga cggaacaggc accccagacc
    19981 actttctcgc tggcctgttg taccgctgcc gctgcactgc gcagggtgaa cggtaaagaa
    20041 aaaccgcctg cagccagcgc cagagaacct atcgcggtag atttaacgag tgttctgcgg
    20101 ctgatgccca ccatttgttc atttttggac ataactcact ccctgttctt tatcgttata
    20161 taaaggttta tatattgaat atttagcgcg ctaacaatag agggagtcta cctattttgg
    20221 gttaagaatt attaatccat atcaatagaa gggtatgagt aataaggtgg gattatgttg
    20281 tatgttcaaa tcgccggatg tgtcgtatcc ggcgtgcagt cgataatgtg ttactgcggt
    20341 tcggcaggcg cgccatcctg ggtagactgc gcgggagcag agacgttacc gctggtggtg
    20401 cgggtgtaga gaattttatg cgtatcatta gcgcaatgcc cgacgacctg ggaatcaggc
    20461 tgatcaacct ggtcattggg tacaatactt aacgtgaagc tgctttcggg tacgccatta
    20521 ttgataatgc gctgtgatat atcgctctgt atgcgctcac aggatcccgg cgcggcgagt
    20581 accgcgggtg aggcgagggc gagcagaagc gcggcacagc aggttgagag tttcatcata
    20641 agctccttac gcgaagataa cttctttaag catagcattt aacgtgtaaa gtactgtatt
    20701 tgctactatg attgagaatc atctctactc tctggtgact gttgtgaaat acaaattact
    20761 gccatgctta ctcgcgatat tcctcacagg atgtgaccgc acagaggtaa cactttcatt
    20821 tacccctgag atggccagtt tctctaatga attcgatttt gatccgctgc gtggtccggt
    20881 aaaagatttc actcagacat taatggatga gcaaggtgaa gtgacgaaac gtgtttctgg
    20941 gactttgtcg gaagaaggct gttttgattc actcgaatta ctggatctgg aaaataatac
    21001 cgtggttgcc ctggtactgg acgccaatta ttaccgtgat gcccagacac tggagaaaag
    21061 agttcgtttg cagggaaaat gccagctagc agaattacct tctgcagggg tgagttggga
    21121 aaccgatgat aatggctttg tgattaaagc cagcagcaaa cagatgcata tggaatatcg
    21181 ctatgatgat cagggttatc cgctgggtaa aaccacgaaa agtaacgaca aaacattatc
    21241 tgtcagcgct acgccatcaa cggatccgat caaaaaatta gattacacag cggttacttt
    21301 tctgaataat caacgagttg gtaatgtaaa acagagctgt gaatatgaca gtcacgccaa
    21361 tccggtggac tgtcagctaa tcattgttga tgaaggagta aaacccgccg tcgaacgggt
    21421 ttacaccatc aaaaatacta tcgattatta ttaatgctat tgtgcggtcg gcttcaggag
    21481 agtctgaccc ggtgttttgt gctctgccag atactgatgc tggaatatgc acatgcgaat
    21541 ggcattccga tattgaccat taataaagaa ctcatgcatc aattcacctt caaccgtaaa
    21601 gccaagcttg cggtaaatgt gaatcgcttt ttcattctct ttatcgacga tcagatacag
    21661 cttatagaga ttaagaacgg tgaagccata gtccattgct aattttgcgg cacgcgttgc
    21721 cagacctttc ccctgatatt ccggggagat aattatctga aattctgcgc ggcgatgaac
    21781 atggttaatt tccaccagct ccaccagacc ggctttttcg ccgtcacatt ccaccacaaa
    21841 gcgccgttcg ctctgatcgt gaatatgctt atcgtacaga tcagagagtt caacaaaggc
    21901 ttcgtagggt tcctcaaacc agtaacgcat cacactggcg ttattgtcga gttgatgtac
    21961 atagcgtaaa tcttcacgct ccagcgggcg tagcttaaca ctgtgggcgc ttggcataac
    22021 gtgtccttac attccttaaa tcaataacag gttagggggt aataacgcgg ccagttcgac
    22081 ggtccaggca gcgcaaagta tttggctccc agtaggcatt gatgttggcg ctttgctcac
    22141 atttatcgcg gttatcaaaa gcggcgtcgg ctttatccca ctctttttca gtgcgtttat
    22201 tcactttctg gcgcagattg cgcgtgtcat tccattgctc tttttccata gcggcgtgct
    22261 ggcggctttg cgcactgtcg ccagactcaa tcaccagttt gttagtttcg gcatgaacag
    22321 ttgtgctcaa tgccattgcg caaggcagca gaaaagcgag caggccaatt cgtttgctga
    22381 gagtaatttt cataattcat tccctgtatg aatgattaaa ggtgattcta caccatccac
    22441 tgcgaacgca aaacgtacca ggagggtgtt tatattgatg atattatgtc gccctataac
    22501 tatacatgat gtcaataagt gacaaagatg attaaaacaa cgttactatt ttttgctact
    22561 gcgctgtgtg aaattattgg atgctttctg ccctggttgt ggttaaaacg aaacgccagt
    22621 atctggctgt tgcttccggc ggggatttct ctggcactgt ttgtctggtt gttaacgttg
    22681 catcctgcgg cgagtgggcg tgtttacgcg gcttatggtg gcgtttatgt ctgcacggcg
    22741 ttgatgtggc tgcgcgttgt ggatggcgtg aaactgagtc tttatgactg gacgggggcg
    22801 ttgattgcgc tttgcggcat gttgatcatt gttgcgggct gggggcgcgc gtaaggacat
    22861 aaatccattt tatcaataag atacaaggaa gtgtcagctg acaaaaggta ttctatttca
    22921 tcttttgtca accattcacg gcgcaaatat acgccttttt ttgtgatcac tccggctttt
    22981 ttcgatcttc atacttgtat ggtagtagct cagttgcgta gatttcatgc atcacgacaa
    23041 gcgatgcaag gaatcgaaca tgaagatcgt aaaggctgaa gtttttgtta cctgtccggg
    23101 gcgtaatttc gtcacattaa aaatcaccac tgaggacggt attacgggcc ttggggatgc
    23161 caccctcaat ggacgtgagc tttccgtggc ctcttatttg caggatcacc tttgtccgca
    23221 gcttattggt cgcgatgcac accgtatcga agatatctgg cagtttttct ataaaggtgc
    23281 ttactggcgt cgcggtccgg tgacgatgtc ggccatttca gcggttgata tggcgctgtg
    23341 ggatattaaa gccaaagctg ccaacatgcc gctttaccag ttactcggcg gcgcgtctcg
    23401 tgaaggggtg atggtttatt gccataccac cggtcacagt attgatgaag ctctggatga
    23461 ttatgcccgt catcaggagc tgggattcaa agccatccgc gtgcagtgcg gaatccctgg
    23521 tatgaaaacc acctacggca tgtcgaaagg caaaggtctg gcttatgaac ccgcaaccaa
    23581 aggacagtgg ccggaagagc agttgtggtc gacggagaaa tacctcgatt tcatgccgaa
    23641 attgtttgac gcggtacgta acaagtttgg ttttaatgaa catttgctgc atgacatgca
    23701 ccatcgctta acgcctattg aagcggcgcg ctttggtaaa agcattgaag attatcgcat
    23761 gttctggatg gaagacccga cgcctgcgga aaaccaggaa tgcttccgtc tcattcgcca
    23821 acataccgtc acacccatcg cggtgggtga agtcttcaac agcatctggg actgcaagca
    23881 actgattgaa gagcaactta tcgattatat ccgcaccacg ctgacccatg caggcggaat
    23941 taccggtatg cgccggattg ccgattttgc ttcgttgtat caggtacgta ctggctcaca
    24001 cggtccttcc gatttgtcac cagtctgcat ggctgcggcg ctgcactttg atctgtgggt
    24061 ccccaatttc ggtgtccagg aatacatggg ttattccgaa caaatgcttg aagtcttccc
    24121 gcacaactgg actttcgata acggctatat gcatccggga gacaaaccgg gtcttggcat
    24181 cgaattcgat gaaaagctgg cggcgaaata tccctatgaa cctgcttatc tgccagtcgc
    24241 acgtctggaa gatggcacgc tgtggaactg gtaaggagta agataatgaa aagcatttta
    24301 attgaaaaac cgaatcaact ggcaattatc gaacgcgaaa tacccacccc gtcagcgggt
    24361 gaagtacgag taaaagtgaa acttgccgga atttgtggtt cagatagcca tatttatcgt
    24421 gggcataatc cttttgcgaa atatccgcgc gtcattggtc atgaattctt tggcgtcatt
    24481 gatgcagtgg gtgaaggcgt ggaaagcgcc agagtcggtg aacgcgttgc tgtcgatccg
    24541 gtggtcagct gtgggcattg ctatccgtgc tctataggta agccgaacgt ttgtacgaca
    24601 ctggctgtat taggtgtgca cgctgacggt ggtttcagtg aatatgccgt ggtgccggca
    24661 aaaaatgcgt ggaaaattcc tgaagcagtg gccgatcaat atgcggtgat gatcgaacct
    24721 tttaccattg cggctaacgt taccggtcat ggtcaaccga ctgaaaatga taccgttctg
    24781 gtttacggtg ccggtccaat cggcctgacg atcgttcagg tattaaaagg cgtctataac
    24841 gttaagaatg tgattgttgc cgatcgcatt gatgaacgac tggaaaaagc gaaagagagc
    24901 ggggcagact gggcgattaa taacagccag acaccgcttg gcgagatttt cgctgaaaaa
    24961 ggcataaagc cgacattaat tatcgatgcg gcttgtcatc cttcaatcct gaaagaagcc
    25021 gtaacgctgg cttctccagc ggcacgtatt gtattgatgg gcttctccag tgaaccgtct
    25081 gaagtgattc agcaaggaat taccggaaaa gaactctcta ttttctcttc acgcttaaat
    25141 gcaaataaat tcccggtcgt tatcgactgg ttaagtaaag ggttaattaa accagaaaaa
    25201 ctaattaccc atacgtttga tttccagcat gttgctgatg ccattagttt atttgaacag
    25261 gatcaaaagc attgctgcaa agtcttactc actttttctg aataatacca ataacggcga
    25321 gtaagtagta cgcatcttac ctctttttta gagataacca ttatgacaat agaaaaacac
    25381 gaaagaagca ctaaggattt ggtgaaagca gcagtatcgg gatggctggg cactgcgctt
    25441 gaatttatgg atttcaagag tcatgcgtgt taactatttg ataaatatta aattaatttt
    25501 tcattgcttc gttatggggc atggttgggg caaactcgct taactgtgta tttaacaaag
    25561 ctacctgtgc attattgttt tcagacatcc actttccgta tacctgaaat accatttgcg
    25621 catctgcatg gcccatctgg tttgctataa atgccgggtt agcaccagct gtcagcgacc
    25681 agcaggcata agtatgtctc gactgatatg attttcgatg gcggagtccg gcacgtttta
    25741 tcgctgcgtc ccacatctgc cttattgagt caacggtaaa atggtcacca taatttttta
    25801 ctctcgctga cacttcaggt tgaaaaacaa aggtgcattt ttgtttttct gttctgccat
    25861 actctctgag gtgaacatca atgatatgct ctttgctcag tctcgttaat gtcatctgac
    25921 tccggagagc gtcgattgct ggcttaataa gatgaatgac ccgattggtt cccgcctgtg
    25981 tttttggtac cgtgaaacgg tcttttgcta aatttctcct gatcatcatt gttccatttt
    26041 tcagatctat gtcctcccat ccaagtgcac acagctcacc agggcgaacg ccagtataaa
    26101 cagaaacaca ccataaattt tttgcttgct gatttctgca cgcatcgata agacggataa
    26161 attcctcccg cgaaagagga tccggaatgg ttcttgattc ctttaatggc gagatcccct
    26221 taaacggatt atctggcagg taaccgttat caacaccaaa ctggaacacg gcgttaagat
    26281 ttgtcatgta attatttaca gttacagccg atctccctgg ttgtgtaaca atatagttac
    26341 ttttggggat ctggtatcca gtcagtagct ctttacgaac ctccagtaat ttttctttat
    26401 taatcgatga ggcaagattt ttttcaccga ttatgctcag gatatttttg atgacggcac
    26461 ggtatgtgtt gagtgatgtt ttggcgactt cagtttcttt cagtgccaga aatttttcag
    26521 ccagttcttt tatggttaaa tcttgtcggg cctcaccaaa tttttccaga ttgcgtgagg
    26581 agggaaactg ttttgcatag tcgaaaacac cagtttttat tgcgtaacaa acagaggagc
    26641 gtagttcacc tgcaacgcgc ctgttttttg ctgtgtcagg aacccccaga ttttccctga
    26701 ctcttacgcc tttataaaca aaccagatac gtaatttccc tccatggttt tccacgcctg
    26761 tcggatattt catttcaact tctctcatta gttagtgtgg cttttagtca agtaagatga
    26821 cgtcttggtc tcgctgatgc ctggcgctca atccagcgat caatttcttc caggttgtaa
    26881 aagcatggac tgttatccca tggcataccg tcatgagcga catgcttata ttcccttcct
    26941 tccataaacg atttttcccg ggcctttttt aacgtacctt tttttattcc tttcagcgca
    27001 attaactgct cttcggatac ccatttgccg ggagagacaa tcatgattac ttcgctcatc
    27061 gatttcttta tctcttacat cagacgagcg ccggttgcag aataccagtc acaaccggcg
    27121 acagttgaac attaagaatc agcctgactc gggatcagtt tttgccagat aactgaaacg
    27181 tattttgcct ggtaacgggc gtcatcaagt gcattatggc gctcaccttc gaatggaata
    27241 gccgttctgg catcgaagtc tatggctttc cccagctcaa cgattgtgcg tacatcgcga
    27301 tcgttgtagt aacgccacgg gcaggggatc ccctgccgtt cgtatgaacg gcgcaaaatc
    27361 gtgttgtcga agttggctcc atttccccaa acctgaacaa aaaattcacc ggagttttcg
    27421 tcgataaatt cccgcaattg taacagtgca tcatctaacg ggatttcatc ggtcataatg
    27481 gcagattgcg cttcacgtga ttgcttaagc caccatttaa tggtgtcccg atcaatgact
    27541 ccgccagcag tttccagatc gatagtctta ctaaattccg gtcccatatc tccggtttgc
    27601 ggatcgaaaa atattgcacc tattgagatg atcggggcat caggattttt tcccatggtt
    27661 tcaaggtcga tcattagatg gtcacacgtc ctgctggtgg atgtgatttc gtgatgaccg
    27721 ttcaccttaa ttgagtgatc tgccgtctcg ccagtttcat tatcgctatt gtgatgctga
    27781 ttgccgccag tgttctcctt gtgtggatgt tcagcgcctt ccatttcctc cggatcatct
    27841 tcctgaactt caacctgata ctcttcatcg aatgtttctt ggtatgttgc gtcgcccatc
    27901 accgcgccac aatcagggca gttgccgccg ccggtctgac cgcaggcggt gcagactttt
    27961 tccacttcct gttgcgccac tggttcaggc tgtttcgttt ctggctcgtt ttgtaacgca
    28021 tttgtgctgt tttgttccgc tttttggtag ttccgttccg attcatgctg gttctggttc
    28081 acagaatcgc gggtctggat ccccttaacc catttcggat cattcgggtc actaatccct
    28141 tcaacaaatt caccacgtga tgcagcaagc aacttatcgg cgtcaggctg gctgatattg
    28201 gctgcctgca taattttgtt tacttcgtca gcggtaactt ttatcggctc tggttgttct
    28261 gaatcttcag cggtatctac attttgcggt aagcccttgt atgtgccatt ttttcgggca
    28321 aaatattctt cttttgtgat ttcagtggcg ccagcagcca gtgccttatc cagaccagaa
    28381 agtttgtttg cgcgaccgta tttttctccg tccttatctg cgaagaggaa atagaacggc
    28441 ccctcacgct ctacagatgg ttcagcttcc ggcgcggttt cattttttgg gatatcagat
    28501 acctcagttt ccactgcatc agtttgtgtt tctgatgact ggagaacatc aacagtgccc
    28561 aggtctgttt cttcattctc aaacacgccc tttgtcgtca ggtattcgca gatatatttg
    28621 ttcagtgcta tgggatcttt gtgaatgtcg atcggacgct cacggacaag gccaaaaata
    28681 gtctggcggt cgtagcgaag ggcatcaggc tgtttgcgca ttgatgccga gatacgcttc
    28741 cagtcttcgc ggtcgttgtc gataacttct ttttttgccc agcgatggat gctgccgtca
    28801 atgtttccgg catccacatc accaggccag agagcgtagg ccagttcgtc atccagtgtt
    28861 ttccatgtct gcttgtattc gcgatgaatg gcagcaatga ccgggctgat ttttcctgtt
    28921 gaattttcag tgtactgttg attggctctg gcgcgggcga gatcaacaac agacgtgtat
    28981 tttccggttt ccttgcgttc accttcgcga cgttttttcc agatgcgcat ctctgcctga
    29041 atttcgggcc atttagcact aggaatacat ttatgcttaa cccacccaat ggcgtgcaac
    29101 ttaagctccg gatacatggc gttaacttct ggcattttca tcaacgcttc aacgatatgt
    29161 ccgtcgaatg ttgccatgtc ttcctgcaac aattcctgtg cgctaataac catatcaacg
    29221 gtgacgtttt cacatgtgtc gaacttaacc atgacagcgt tctgtacttc aggggccagc
    29281 ttgtcaaaag tgacgttcat cggatcggat tcagtctcaa ccgggacaaa agaagcagac
    29341 tcctcatccc agcgtttttc ctgcatatat tcagcatccc atgaatcgag ggcagggcgg
    29401 ggtatgccag gtttatcctc gcagacaata aatttataag cgcagtcctg agcagccgga
    29461 taatgttcca ggaactgcca gtgaaatttt gctcgagcac ggcgttcgtc gccagcttca
    29521 atggctgtgg ctacagccac agcgccttct tcccttgttg ccagttcgtc aggaatagcg
    29581 gcgcaaataa agactttact cattttgttt taacctcatg acagatttaa ggatgaacaa
    29641 atccctgcca ttgctggcat ataagaatga aaccggatat ttattacgga actgttttaa
    29701 agacctgccg ggatttcgat attatcctgg tgaataactt tatcgaccgg gtaacagtta
    29761 ccgggaattt tctgttcggt tgctgcagtc atacactcct gcattgtcct gtgaacactg
    29821 actgcaatat caactggctc tccggaaaca agaaaaactg tcagaacaag cgcaaatgct
    29881 gaattcattg tgcacatcct tttggcatca gacgtaaacg agccagcatt gaaacaatgc
    29941 atattttatt taatagctcc cgttcttgtt ttctcttgtt aatggcatct tcagtaaata
    30001 cagtgttact gatagtgaca ccaatttcaa aacaaccttc agacgtatta acgtttggta
    30061 ataacgtttt cattatcgcg tcctcaacaa tgaattttgt gatgcagtgc ctggtgcctc
    30121 caggtgacgt taaccagtta acaattaacg ccggatacag agaatccacc cataacactg
    30181 tttttggttt taactgttcc gcgtgcgctt agccgcattc accgcatcac aaaattcact
    30241 ttaaaaacgg cggcagagca gtcacggagt aaaactgata ccgccaaacg tcaccagaaa
    30301 attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca acctgacaac
    30361 ccggtgtcct caacggggaa ggaataaccc cgccatactt accgccgcgc catttcgcgg
    30421 gttgccacaa ccggaagcgc acggtcgaat taaatttaac gacaccgtac agtgagacga
    30481 acttcgccgt gcgctttcgt gttgtgtgcc tgcttttaac cacgtcaggc gaggtggtat
    30541 ccttcttatt ccgaataacc aagaaggaaa tctatatgac taaagaagaa tttgtctctt
    30601 atatttttga taaaacggtt gaaatgtatg ccgctactta cgggtcctgt aatcctctga
    30661 ataaaccaga ggggaaagat gatttcgaca aaatttaccg cttcttggag gaccgctata
    30721 tcaaaaggtt agaggacgca gggatcaaat ccccagtgaa gtcaccattg tcctgagaac
    30781 ttgcaggacg tcatgatcgt aacttccatc caaaccgcga cggcaaattg cttctcttat
    30841 taccggaagc agttcgctgg aaatctcggt gcatatttca cctgataata cgcctggctc
    30901 aagtgagaat attggtgaac ttacggtctt ggtctcgaca gtttcagagt cagtgccaac
    30961 attataaagc tcaacgaaag cggtcttgat tttccgggcc agatcttttg ctggctcgct
    31021 tgcaatatct ttcccgattt ctcgcagcac agaatgcaat gtatgagctg ctgttttctg
    31081 tacatcagac ggtaaatctt taaattccat cgtcagcctc atcagtcagt gtttctggct
    31141 aaccagcaac gcgcgccaga ttcggtttta aacgttttgc ttttggtata tgtcatcgcg
    31201 gtgaacgtat cgtcctggtt ggggaacacg ccacatacca gagattcgct gttgccaaga
    31261 tcgatagtat ccatgctgac ctcatttccc cttaacgccg gggtagcgga actgtttgct
    31321 gagaacaccg tgcggtttgt tgatggaggt aatttagttt tctcattatt tttcgtcaag
    31381 gttttttgat gagaaaactc aagtattgac gcaagataaa gccaatgcat tgaaatgtaa
    31441 ggctttaaaa ttttgtgaag ggatgattat tgatgcttgt tgcgtttgcg agcctctaat
    31501 agctcggtga acaggcgatt aaaattctca acgcgggcgc ggagttcgct gagttgtgct
    31561 tgttgctctg atttaggaag tgcgcgatac aaacgcagca tctctaactc atcttccgat
    31621 aagtctaagg cactatcgag tgaaacaggg ggagctggtg ttttgtcctc gtcgccaaac
    31681 agtatccagg ttggtgaaca ttgcaatacc tcggcgaggc gatgcaaatt ttgcccgcgc
    31741 ggggctgtat ggtcactttc ccataatgaa attgatgagc cagatacgcc agcagctttg
    31801 cttaaaccgt tttgacttaa gcctacctgc ttacgtcttt ctctaatgcg ttgacctaaa
    31861 gttttctcgt tcatatttag atatcttaat aacccttgac ttgagattcc ttgagtgatt
    31921 agcattgaga aaactcaata ttggaggtgc gatgtttaaa tcagacgtaa ttaattttta
    31981 cggtacgaaa gccaaagtag cgaaagctgc tggcgttgac ccatctgctg tttctcaatg
    32041 gcaagagctg gttcctgaag gtcgcgcgat gcgtctacag gaggcatctg gcggcgagct
    32101 tctgtatgat cccaaggttt atgacgaata tcgtaagacg aagcgggcgg ggcggttgaa
    32161 caatgaaaat cactcctgaa caggctcgtg aggctctgga tgcctggata tgtcgaccag
    32221 gaatgacaca ggagcaggcg acgatattaa tcactgaagc attctgggct ttgaaagagc
    32281 gcccgaacat cgatgttcag cgtgtcacag atgaaggtgg cgcggttgat cagcgagcgc
    32341 ttggcgttaa tcgagtgaag atattcgaac gctggaaggc tatcgacacc agggataagc
    32401 gtgaaaagtt cacggcgcta gtgcctgcaa ttatggaggc tatccggatt aatgatttca
    32461 ggttgtatcg tgaaattagt gacggaaaaa gcatcacgta catgatcgcc gggttaaaca
    32521 aagaatatgg cgatgtggtg gagtccggac tgctttttgc tgatcctgcc gtagtggatc
    32581 gtgaaactga cgaacttata gaaaaagcaa ttgctttcaa acttgcgtat cgacagcaat
    32641 accaacaaaa agctggatgg aattatgagc cttctttttg ctgaacgccc actggttata
    32701 aacacacagc ttgcgatgaa gattggctta aatgaagcca ttgtgttgca gcagttgcat
    32761 tactggttga gagataccaa ttccggcatg gaatgtgatg gtgttcgctg gatttacaac
    32821 acaacggaac aatggctgga acagttccca ttctggtcag agtcaacgtt aaagcgcgcg
    32881 tttgcaagtc tgaaaacgct ggggcttttg cgttgtgaaa agctcaataa atcaaagcgc
    32941 gatatgacca atttctacac gattaactat gggagcgagc ttttagatga tggcaaattg
    33001 agcgaatcca tcggttcaaa atgcgccgct ccatcaggtc aaaatgacac gatggaagag
    33061 gtcaaaatga aacgctccat tggttcaaaa cgacccaatg tcatcgggtc aaaatggccc
    33121 gatgatccta cagagaatac aacagagatt actacagaga ataaaaacac ttttcgtccg
    33181 gaagcttcgc aaccggaccc gcagacggct gaacaggatt ttttaatccg gcaccctggc
    33241 gcagttgtgt ttagtgcgaa aaaacgccag tggggtagcc aggaggatct ggcgtgtgcg
    33301 cagtggatat gggggcggat cgtgggtctc tacgaacagg ccgccagtga tgatggcgag
    33361 atcatgcgac caaaagagcc taactggact gtctgggcca atgatgtgcg cacaatgcgg
    33421 atgctggatg gcagaagcca cagacaaatt tgtgaaatgt ttggtcgggt acagcgggat
    33481 ccattctggg taaaaaacat catgagcccg tcaaagctcc gcgaaaaatg ggacgaactg
    33541 gtcatccgcc tggggcgttc acctgtacag cgttgtgtta atcatatttc tgaaccggat
    33601 acagaaattc cgcctggttt cagaggataa gttttgattt caggtcatga ggtaatttta
    33661 agggggactt gtggcaaaag tttttacaca agaagagcgg gaaaaaatta aagggcaggt
    33721 ggtggaactc gtgcgccaga gcggtcgtga gacgttacgg caactggaag ctaaaacagg
    33781 tgcgactaga tatctgatga gcgttcttgc cagagagctg gtagccagtg gcgatgtata
    33841 caactccggc tacgggttat tcccgtctga acaggcgcgt aaggactggc aaaacgcccg
    33901 caaaaaactc tcgagggcaa aggtgaagaa aacagctgtg gttgatccgg accttatctg
    33961 gtcgttacct gatggagaaa tacgtcgcta cgacagtcgc ctaaacataa tctgtcgcga
    34021 gtgccggatg agtgaagtta tgcagcgcat actggcattt tatcagggat aatgttaggt
    34081 attttagacg ttactagatt aaaaagcatt agttcaggag tgaattgaca ttctcatttt
    34141 tcatggcaca gggtagatct ggcgtggttg tccgctttgt gccaaaagcg gacatcgaat
    34201 aatttcagca gcactcaacc ttattaacca aatcgcctca actcatatgg acatgcatcc
    34261 ggaagtgcca acttcgtaaa cttattcact ttaagtctga gcctcactct cttggccata
    34321 agtggcttgc cagtatctcg ccgtagggtt cattagaatg tgcaatacaa gccaccatgc
    34381 aaaaatgcag ggaggttgat tgtagtaacc ggcaggcaca tatccgtgag ctagttgatt
    34441 tcgtaattca ccatagattt tatctaccag taaggcctga agattgccaa ttatgtctac
    34501 gccgaaagct tcaataatca gtggattatc tagtaatccc ttgagtccat ttcgctcttg
    34561 cgaaccgtcc ccgtgaagct gcgatggttc ctcaccttta attttagcca catatctcag
    34621 actattttcg atctggggaa tcaacacgtg gcatgccgtc ataaaatctc cattgaaacc
    34681 gcaaaccata cccttgatga aaaactcttc atgcccaaac gggatgaagg gatgattgat
    34741 gaacatatcg cgaaaaaacg cctccgagat tggatatttc attgtgatct catctgtagc
    34801 aggaataatt tgcccagcta ctgccaactc atggtcgata cgcattctcg tcatcattat
    34861 gggccaaata actgccccat ttgcatcatc aatccccaat cctgcgggta tgcgagcaag
    34921 tgtcattccc tcatgatcaa tatgtgttga tccaaacatc caagctatgc tgttggcctt
    34981 ttgttctatt gcttgagcct taagtctctc aatgctggtt ggccgacaga ccagcatcgc
    35041 aagtctgaaa accatatcaa acaaatctct tccgactacc cgactcttgg cttgatggac
    35101 aatctcacta atatcttgag ggggagactg aaggatagac atctggtgac gggattcgat
    35161 ttggtaatca cgcatttctt catacagcgc aagacgttct attcgggttc ctggaatatt
    35221 agccagtgct tcaatagcat tctgcatgca ggctgccgaa atcataccgc catcttggaa
    35281 ttttgactct tccagatgtg tatcggcaag aagccgccag aattcaaact gtgtatctct
    35341 gtcacagcac ctattcgcaa tcgggattgc ggttttacag gcatcgacgg caaatgaaaa
    35401 taatttttga gccagatagt tttttgtcag aaacaggagt tcctgggcaa taaaagatgg
    35461 ctcactcact ttacagtcga tatcgagttg caggagtcgc agcggataag gtgaatcgcc
    35521 ttgttctctg gtacgttcat actctgctag tagatgtgcc gaaagctgat caaattcatc
    35581 cctgaaatca gtattctttc gaaaaaaaca acacagccta agcgcacgct ctaggcgttc
    35641 taaagcacta acccacgtcc cttgcgatgt cataatagct atactggacg cataataagc
    35701 acggactgcc agcaaaggaa aacggattcc accaatttta cgaacccaaa gtgtatcagc
    35761 aatacgagct tgaagttcgg gtagctctat cgtcgggcaa aattgagaaa gtacctccag
    35821 cgacaacccg ttaatgtcat ccggaattgc agatctacct tggctgcttt gaaagcacgg
    35881 accaaatggt acctgtggat tttcgggacg aaagtgaaaa ccgcaaatat gggttagcag
    35941 tcttatctcc gctcttccat cacctaggct agaaagatac tttgtgatat caaagcaggt
    36001 aactggcagc ttactctgga ttaaggggga taaatcaata gtgttaagtt cgtcgaggct
    36061 gatattaata tcgtccatgt cgtcaccttg atgatttgtt aatgcggggc aacattaata
    36121 aatgtacttg agaccgcaat ttgtcgatgg agccaacatt tataactatg tgcttctacc
    36181 ctctgaattt ttacatgtga attgctagct tgaatagctc tatcattata tacgtggcac
    36241 taaattatgg ctgtttttcg ctcaaagcgg actagaaggt tagcttgtgt cggactttgc
    36301 gtattaaaag aagtgctggt ggtgactagt ggttgtgccc catttccaca gaaaaaatca
    36361 gagaaactat acccaatagt tgtattgaat cactgacgag acagcctcat attcatcagg
    36421 actggtctac gtccaataca ggaggttgtg gtgctggttc tcaaatgtgc gctggctatt
    36481 acggctgtaa tggcgattta ttgtcttgct attgttctta tggatcgcct ttctgactga
    36541 tttcatattg gcgaggtaac ggtagttaag tagaatggct gcgggtgctt gaggctatct
    36601 gcctcgggca tgaacaccaa aggcagatag agaaaagccc cagttaacat tacgcgtcct
    36661 gcaagacgct taacattaat ctgaggccaa tttcatgcta gacacatgta ggttagcctc
    36721 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
    36781 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
    36841 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga
    36901 ggagtaagag acccggcgag ggagaaatcc ctcgccacct ctgatgtggc aggcatcctc
    36961 aacgcacccg cacttaaccc gcttcggcgg gtttttgttt ttattttcaa cgcgtttgaa
    37021 gttctggacg gtgccggaat agaatcaaaa atacttaagt agcgcgcagg gataagaggg
    37081 atggtccctt aaaggggaga gctaattatc cggaaggatt ctgatgatga acatcgaaga
    37141 actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa atggaaatct
    37201 tgttagtcat taccgcatta tgtgtttgcg aaagaatggg gctgcgttaa ttaattttgt
    37261 ggatggtcga gtgacagacg gatttatctt gcgcgaaggt gagtttgtca cttcattaca
    37321 ggcactgaaa gagatcggaa taaaagcagg cttttcagct tttgcagaag aataaactca
    37381 tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc ggagaaaacc
    37441 gatggcacgc aacgtaaaat attacaattc tgataattcg cccgttcttg cctgcacgca
    37501 cgggcggtat tctcacgcat tcaagtctga atggttccag caccctccat gcactgcaga
    37561 acaggccgaa tggctgattc attcttaccg caggcgcggg ttcgaggtta agaaagctct
    37621 cagtctcgac tatcggcact ggataatctc tgtcaggctg ccttattccg aacgcccacc
    37681 acgtgcgtcc cgcactttcc agcaacggat ctggaggtaa cgtgcgggta ttacttagac
    37741 ctgttctggt gcctgagctt gggctggtgg tccttaagcc gggccgtgaa tccatacaga
    37801 tatttcataa tcctcgagtg ctggtggaac cggaaccaaa aagcatgcgt aatctgccat
    37861 ccggagtcgt tcctgccgtt cgccagccgc tggcggaaga caaaacattg ctgccgtttt
    37921 ttagtaacga acgggtgatt cgtgctgctg gcggcgttgg cgcattgtct gactggctat
    37981 tacgtcatgt tacatcctgc cagtggccta atggcgatta ccatcacact gaaacagtca
    38041 ttcaccgtta tggtaccggc gcaatggtgt tgtgctggca ctgcgacaac caactgcgtg
    38101 accagacatc ggaatcactg gagctgcttg ctcaacaaaa tctgacagca tgggtgattg
    38161 acgtcatccg tcacgcaata agcggtacgc aggagcggga attatctttg gctgaattat
    38221 cctggtgggc ggtctgcaat caggtggtgg atgcactacc tgaggctgta tcgcgtcgtt
    38281 cgctgggatt accagcggaa aaaatctgct cggtgtaccg cgaaagcgac atcgtaccgg
    38341 gagagcagac cgccaccagc atattgaaac aacgcacaaa aaatcttgca ccgttgcctt
    38401 acgcccacca gcaacaaaaa ccaccacagg aaaagacggt ggtaagcatc aacgttgatc
    38461 cagagtctcc ggaatctttc atgaagctgc ctaaacgtcg ccgctgggtt aaggagaaat
    38521 acactcgttg ggttaagaca cagccgtgtg cttgctgcgg tatgccagcc gacgatccgc
    38581 atcatctgat tggtcacggg cagggcggaa tgggaacaaa agcacatgat ctctttgtgt
    38641 tgcctttgtg cagaaagcat cacaacgagc tgcatacgga tacagtggca tttgaagaga
    38701 agtatggctc ccaactggag ctgatatttc gttttatcga tcgcgcgctg gcaactggcg
    38761 tactggcgta agtggagaac gagcatgaac cttgaagcct taccaaaata ttactcccca
    38821 aaatctccaa aattgagcga tgacgctcca gcgacaggca ccggttgttt aacaattacg
    38881 gatgtaatgg cagcgcaggg gatggtgcag tcgaaagcac cacttgggtt ggccttattt
    38941 ctggcaaaag ttggtgttca ggacgctcag tttgcgattg aaggcctgct aaattacgcg
    39001 atggcactgg ataacccgac attgaacaaa ttgagtgaag aaatccggtt acagattatt
    39061 ccttacctcg tgaattttgc ctttgctgat tactccaggt ctgcggcaag taaggctcgc
    39121 tgtgagcatt gttcaggtac gggattttat aatgtattgc gcgaagtggt gaaacactac
    39181 agacgcgggg aatctgtaat caaggaagaa tgggtgaagg aactatgtca gcattgccat
    39241 ggtaagggcg aagtcagcac agcgtgcaga gggtgtaagg ggaaagggat tgttctggat
    39301 gaaaaaagaa cccggtttca tggcgtaccg gtatataaga tttgtgggcg ttgtaatgga
    39361 aaccggttta gtcgtttacc gaccacgctg gcacgacgtc atgtccagaa gctggtacca
    39421 gacctgaccg attatcagtg gtataagggg tatgcggacg tcattggtaa actggtaaca
    39481 aagtgctggc aggaagaagc atacgcggaa gcgcaattga ggaaggtgac gagataaatg
    39541 atttttgctg aagatggcga catgatgttt gcatttttca aaaaacatgg ataagatttt
    39601 ctcaacgatg ggctttgtgt atccgacgtt tagaaaaaag tagaaaaccc gcttataagc
    39661 gggtttttgt gctttaaatg gggcaataga gatattgaat ctcatcccgg gataaacatt
    39721 ggcagttgaa ggtccacgcg aaccatttat ccagcaaaat tccacgcgta atcctgtggt
    39781 aatttcttct gcatctcgaa gattgagagc tgaaacgtga agctgggcat cgatacgcca
    39841 tcggatggga atataagacc tttgctgctt ttgtagtcaa agtttttgac aattcctgtc
    39901 attttagggg acagaaaaac tccttaatac tgataacctg gtgcaccata cacacgttcc
    39961 tggagaaaac tacttttttg ataggattga aggtggctgg atgtctaaaa taaacattgc
    40021 ttcatatgtt caactatgag ttaatgactg cgtcggtttg aagaacagac gatatacgaa
    40081 gtagtttact aaagcagttc tcatttcagg tgttattcac ttattccttc tttgagtctc
    40141 tccaattaag tacgaagtcg tttctgttat gcaaaccatt tatgccgaaa ggctcaagtt
    40201 aaggaatgta gaatgtcaaa taaaatgact ggtttagtaa aatggtttaa cgctgataaa
    40261 ggtttcggct ttatttctcc tgttgatggt agtaaagatg tgtttgtgca tttttctgcg
    40321 attcagaatg ataattatcg aaccttattt gaaggtcaaa aggttacctt ctctatagag
    40381 agtggtgcta aaggtcctgc agcagcaaat gtcatcatta ctgattaaaa ttcatcgctc
    40441 gtctgtatac gataacgaag aaggctgatg cctgagtaga gatacggaca gagtagtgaa
    40501 tattggatct ttttaataaa aagtaaggag gtccaataca tgaaacaatg gctagcatat
    40561 ttggcaaaat cttaatcagg aaaagtatgc taaccattgt ggtgaagtgc aggtttgctg
    40621 catgaatagt tttacagcag aagctaactg ctggcatggc aaaacaaagt gcgtaagtgg
    40681 atgactccca caaaaagcac cacaatctca aacccgctca ggcgggtttt ttattatctg
    40741 ctttaaatat attatta
//
LOCUS       DARNVM010000019        39710 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.019, whole genome shotgun sequence.
ACCESSION   DARNVM010000019 DARNVM010000000
VERSION     DARNVM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.019"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            139..3867
                     /gene="yfaL"
                     /locus_tag="SI621_001008"
     CDS             139..3867
                     /gene="yfaL"
                     /locus_tag="SI621_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HEI4063750.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVIDSCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMNNNASGGAVFLQQGAEFSLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSDNVAGGYGGAIYSSGTNDTGVVDLSVTNA
                     VFRNNIANDGKGGAIYTINNDVYLSEVIFDNNQAYTSTSYSDGDGGAIDVTDNNSNIT
                     HPSGFTIINNTAFTNNSAEGYGGAIYTNSVTAPYLIDISVDDSYSQNNGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSG
                     DLVLNANNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQS
                     GTSYNDGLEVSGSGGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVIDAGDGLVSLANN
                     NSYLGTTQIASGTLVVSDNSQLGNTDENRQLIFTDSQQQSEMEITADVDTRSEAAGHG
                     RDIEMRADGEVAVDAGVDTQWGGLMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFKTGADQDIQSIDVTSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVVIN
                     PITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEATPPSPPDPDPTPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQAFVMERHDHA
                     GGDGQTLNLRVIGGNYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGADGEWMLGAVGGY
                     SDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFNNDVSE
                     QDDGTDHYHSSGIIASLEAGYQWLPGRGVVVEPQAQVIYQGVQQDDFTAANHARVSQS
                     QGDDIQTRLGLHSEWRTSVHVIPTLDLNYYHDPHATKIEEDGSTISDEAVKQRGEIKV
                     GVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3994..4716)
                     /locus_tag="SI621_001009"
     CDS             complement(3994..4716)
                     /locus_tag="SI621_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HEI4063751.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4863..7490
                     /gene="gyrA"
                     /locus_tag="SI621_001010"
     CDS             4863..7490
                     /gene="gyrA"
                     /locus_tag="SI621_001010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HEI4063752.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7639..9327
                     /locus_tag="SI621_001011"
     CDS             7639..9327
                     /locus_tag="SI621_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HEI4063753.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKTQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPDAAWQWLPITWQPL"
     gene            9324..9947
                     /locus_tag="SI621_001012"
     CDS             9324..9947
                     /locus_tag="SI621_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HEI4063754.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKVHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9971..14485
                     /locus_tag="SI621_001013"
     CDS             9971..14485
                     /locus_tag="SI621_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEI4063755.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKDFKTGEAVSGKLQLLYPDGEPVK
                     DASVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSDAVSDDKGNVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTSAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAIPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVIDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMSGDGLVGQGSAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESQLADA
                     VANVLTMTLLKKAQLKSTVMPKVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14486..16135
                     /locus_tag="SI621_001014"
     CDS             14486..16135
                     /locus_tag="SI621_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HEI4063756.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVDSGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSDKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWGQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     SCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16140..16916
                     /locus_tag="SI621_001015"
     CDS             16140..16916
                     /locus_tag="SI621_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HEI4063757.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGVIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16990..18174)
                     /gene="atoB"
                     /locus_tag="SI621_001016"
     CDS             complement(16990..18174)
                     /gene="atoB"
                     /locus_tag="SI621_001016"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEI4063758.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18205..19527)
                     /locus_tag="SI621_001017"
     CDS             complement(18205..19527)
                     /locus_tag="SI621_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEI4063759.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVVGLTLF"
     gene            complement(19524..20174)
                     /gene="atoA"
                     /locus_tag="SI621_001018"
     CDS             complement(19524..20174)
                     /gene="atoA"
                     /locus_tag="SI621_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HEI4063760.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVCAKTEARFEVAADLNTQRGDL"
     gene            complement(20174..20836)
                     /gene="atoD"
                     /locus_tag="SI621_001019"
     CDS             complement(20174..20836)
                     /gene="atoD"
                     /locus_tag="SI621_001019"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HEI4063761.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSCLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21032..22417)
                     /gene="atoC"
                     /locus_tag="SI621_001020"
     CDS             complement(21032..22417)
                     /gene="atoC"
                     /locus_tag="SI621_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HEI4063762.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTSPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22414..24240)
                     /gene="atoS"
                     /locus_tag="SI621_001021"
     CDS             complement(22414..24240)
                     /gene="atoS"
                     /locus_tag="SI621_001021"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HEI4063763.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24407..27256
                     /gene="rcsC"
                     /locus_tag="SI621_001022"
     CDS             24407..27256
                     /gene="rcsC"
                     /locus_tag="SI621_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HEI4063764.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRTDGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GIDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(27342..27992)
                     /gene="rcsB"
                     /locus_tag="SI621_001023"
     CDS             complement(27342..27992)
                     /gene="rcsB"
                     /locus_tag="SI621_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HEI4063765.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28009..30681)
                     /gene="rcsD"
                     /locus_tag="SI621_001024"
     CDS             complement(28009..30681)
                     /gene="rcsD"
                     /locus_tag="SI621_001024"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HEI4063766.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQALIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31420..32514
                     /locus_tag="SI621_001025"
     CDS             31420..32514
                     /locus_tag="SI621_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HEI4063767.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKVDGLHYF
                     SDNDSKDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNEPESDNSSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYDSDNFMQQRGNGFATYRNTDFFGL
                     VDGLDFAVQYQGKNGSAHGEGMTTNGRDDVFEQNGDGVGGSITYNYEGFGIGAAVSSS
                     KRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDY
                     KINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            32626..33681
                     /gene="apbE"
                     /locus_tag="SI621_001026"
     CDS             32626..33681
                     /gene="apbE"
                     /locus_tag="SI621_001026"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HEI4063768.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKTQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LSVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEDDSF
                     KTWMSPQFKSFLISEKN"
     gene            33755..34819
                     /gene="ada"
                     /locus_tag="SI621_001027"
     CDS             33755..34819
                     /gene="ada"
                     /locus_tag="SI621_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEI4063769.1"
                     /translation="MKNTTCLTDDQRWQSVLARDPNTDGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQTWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIHGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34819..35469
                     /gene="alkB"
                     /locus_tag="SI621_001028"
     CDS             34819..35469
                     /gene="alkB"
                     /locus_tag="SI621_001028"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEI4063770.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35545..37188
                     /gene="yojI"
                     /locus_tag="SI621_001029"
     CDS             35545..37188
                     /gene="yojI"
                     /locus_tag="SI621_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HEI4063771.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYTPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGNPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            37406..39052
                     /gene="mqo"
                     /locus_tag="SI621_001030"
     CDS             37406..39052
                     /gene="mqo"
                     /locus_tag="SI621_001030"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEI4063772.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(39202..39690)
                     /gene="eco"
                     /locus_tag="SI621_001031"
     CDS             complement(39202..39690)
                     /gene="eco"
                     /locus_tag="SI621_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HEI4063773.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         9677 a         9775 c        10679 g         9579 t
ORIGIN      
        1 atgacgcact gatattattc atgaaataat aacaaaatag ccattgcacc gggttgaaca
       61 atttacaaaa gaaaaataac ccatatcgta taatgcgatt aaatatgccg tcttatagaa
      121 aatgatggtc agattaatat gcggattatt tttctacgca aggagtattt atctttactc
      181 ccgtcaatga ttgcatctct tttctctgct aacggtgtcg cggcggtcat tgattcatgt
      241 cagggatatg atatcaaagc gagttgtcac gccagcaggc aaagcctttc aggcattacg
      301 caggactgga gcatcgccga tgggcaatgg ctggtttttt cggatatgaa caataacgcc
      361 agcggtgggg ccgtattttt gcaacaagga gcggagtttt ctctatcgcc agaaaatgaa
      421 actggaatga ctctgtttgc caataatact gtctcaggag aatataataa cggcggggca
      481 atatttgcta aagaaaactc aacgctgaat cttactgatg ttattttttc cgataacgtt
      541 gcaggtggct atggtggcgc aatctattct tctggtacta acgataccgg tgtcgtcgat
      601 ttaagtgtca ctaacgccgt gtttcgcaat aacatcgcta atgacggcaa aggtggtgca
      661 atttatacca tcaacaatga cgtctattta agtgaagtta tttttgataa caaccaggca
      721 tatacatcaa caagttacag tgatggcgat ggaggggcaa tcgatgtcac cgataataat
      781 agcaatatca cgcatccgtc aggtttcaca ataataaata acaccgcatt tacaaataac
      841 tctgctgagg gatatggtgg ggcgatttat accaatagcg tgacggctcc ctatcttatt
      901 gatatttcag ttgatgacag ctacagccag aacaacggcg tgttagtgga tgagaacaac
      961 agcgcagcag gttatgggga tggtccttcc actgcggcgg gcggctttat gtatctcggc
     1021 ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttatcgg caatacagag
     1081 aatgacggag ctgttgactc tattgctggt accggggtaa tcaccaaaac aggttccggc
     1141 gatctggtac ttaatgcaaa taacaatgac tttactggcg agatgcagat agaaaacggt
     1201 gaagttaccc tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
     1261 gatccgcaag actgctacgg tctgacgata gggagtattg atcagtatca gaatcaggct
     1321 gagctaaacg ttggctcgac ccaacaaact tttgtgcacg cattgacggg ctttcagaat
     1381 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggcag ttttgctggc
     1441 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctacgt gctggcaggg
     1501 gcacagtcga tggcgctaac tggcgatata gtcgttgatg atggcgcggt gcttacgctg
     1561 gaaggcgacg cggcagatct tgccgctctc caggacgatc cgcagtcgat cgtgttaaac
     1621 ggcggcgtgc ttgatctctc tgatttcgcc acctggcaga gcggcacatc atacaacgat
     1681 ggccttgaag tcagcggcag cggcggtacg gttatcggca gtcaggatgt ggtggatctc
     1741 gcaggcggcg acaatttgca tatcggcggc gacgggaaag atggcgtcta cgtggtgatc
     1801 gatgcgggcg acgggctggt tagcctggca aacaataata gttacctggg caccacgcaa
     1861 atcgcttccg gtacgctggt ggtgagcgac aactcgcagc ttggcaatac cgacgaaaac
     1921 cgtcagctca tctttactga tagccaacaa caaagtgaga tggaaattac cgctgacgtt
     1981 gatactcgtt cagaggcagc aggtcacggg cgtgatattg aaatgcgcgc cgacggtgaa
     2041 gtggcggttg atgcgggggt agacacgcag tggggcggac tgatggctga cagcagcggg
     2101 cagcatcagg atgagggtag cacattgact aaaacggggg cgggtacgct ggaactgacc
     2161 gccagcggta cgacgcagtc ggcggtacgt gtcgaagaag gcaccctgaa aggcgatgtt
     2221 gcggatatct tcccttatgc ttcgtcgctg tgggtcggtg acggggcaac gttcaagact
     2281 ggcgcggatc aggatattca gtcaattgat gttacttcca gcggcactat cgacatcagc
     2341 gatggtacgg ttttgcgcct gaccgggcag gatacttccg tcgcccttaa tgcctcactg
     2401 tttaacggcg atgggacgct ggtgaatgcc accgatggcg tgacgttgac aggcgagctt
     2461 aacaccaacc ttgaaactga cagcctgact tatctttccg acgtaacagt taatggcaat
     2521 ctgaccaata cgtccggtgc ggtcagcctg caaaatggcg tcgccggcga cacgctgacg
     2581 gtaaacggtg attataccgg cggtggtacg ctactgctcg atagcgaatt aaacggcgat
     2641 gactcggcaa gtgaccaact ggtgttgaac ggtaatactg ctggcaacac gaccgtggta
     2701 attaatccca ttacgggtat tggtgagccg acatctacag gcattaaagt ggttgatttc
     2761 gcagccgatc caacgcaatt tcaaaacaat gcgcagttca gtctggcggg cagcggctac
     2821 gtcaatatgg gagcgtatga ctacacgctg gtggaagata acaacgactg gtatctgcga
     2881 tcgcaagaag caacgccacc atcgccacct gatccagacc cgactcctga tccaacaccc
     2941 gacccggaac ctacgcctgc ctaccagccg gtgttgaatg ccaaagttgg tggatatttt
     3001 aataacctgc gggcggcaaa tcaggcgttt gtgatggagc gacacgatca cgctggtggc
     3061 gatggtcaga cgctgaattt acgtgttatc ggcggaaatt atcattacac agcagcgggg
     3121 caactggctc agcatgaaga cacttctacg gtgcagctta gcggcgactt gtttagcggg
     3181 cgctggggcg cggatggcga gtggatgctt ggggcggttg gtggctacag tgataaccag
     3241 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     3301 gttggtctga cctcaagctg gtttcagcac ggtaatcaga agcagggggc ctggctggat
     3361 agctggctgc aatacgcgtg gtttaacaat gatgtttccg aacaagacga tggcacagat
     3421 cattaccatt cgtcggggat tatcgcctcg ctggaagcgg ggtatcagtg gttaccgggg
     3481 cgtggtgtgg tggttgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     3541 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gtgatgatat tcaaacgcgg
     3601 ctgggtttac acagcgaatg gcgtacctct gttcatgtca taccaacatt agatctgaat
     3661 tattatcacg atccccatgc gacgaaaatt gaagaagatg gcagcactat cagtgacgaa
     3721 gcggtaaaac aacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     3781 tccctgcgtg gtagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     3841 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc ggcactattg ccggatgcgg
     3901 cgtaaacgcc ttatctggcc tacgggagca tttgtaggcc tgataagacg cggcaagcgt
     3961 cgcatcaggc attggcgcgg caaaacaaca acctcactta ttctgcgtgt gcagcatata
     4021 gttcacgtcc acgccggggc cgagcttaaa ggaattagtg atcgggttat aatgcagccc
     4081 ggtcatatga cgctctttga ggcttgtctg atccacccag cccagcagtt ctgccggttt
     4141 tataaatttc ttcacgtcgt gcgtgccttt gggcaccatg cgcaaaatat attcagcacc
     4201 aaccaccgcc atcagccacg acttgccgtt gcggttaagc gtcgagaaaa aaacatcgcc
     4261 gcccggtttc accagttgcg cacaggctct aaccactgac tgcggatcgg gaacgtgctc
     4321 cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg cgtgctcttc
     4381 cacggtttcc tgcacgtact ctacctgaat gccactttcc agcgcgtgca actttgccac
     4441 ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc gcgccatact
     4501 ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa ataaaccgcc
     4561 agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcagcg gtttgaactc
     4621 accttcaaga tcccaccagc gggaggcgac agcttcaaat ttagcgatct cttcgtggtc
     4681 tacgttatgg tttaccggcg atttttcggc attcattggc acttctactc cgtaattggc
     4741 aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt acctcaaact
     4801 gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg gatagcggtt
     4861 agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag ctgaagagct
     4921 cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat gtccgagatg
     4981 gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc aatgactgga
     5041 acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa taccatcccc
     5101 atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc tcgctgcgtt
     5161 acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct gcggcggcaa
     5221 tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc gatctcgaaa
     5281 aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc gacgtcatgc
     5341 caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta ggtatggcaa
     5401 ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg tatatcgatg
     5461 atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac ttcccgacgg
     5521 cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt cgcggcaagg
     5581 tatatatccg cgcccgtgct gaagtggaag ttgacgccaa aaccggacgt gaaactatta
     5641 tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag attgcggaac
     5701 tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct gacaaagacg
     5761 gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg ctcaacaacc
     5821 tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca ttgcaccatg
     5881 gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt caccgccgtg
     5941 aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt gctcatatcc
     6001 ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg atccgtcatg
     6061 cgccgacgcc tgcagaagcg aaaactgcgc tggttgctaa tccgtggcag ctgggcaacg
     6121 ttgccgcgat gctggaacgt gcgggcgatg atgctgcgcg tccggaatgg ctggagccag
     6181 agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag gcgattttgg
     6241 atctgcgttt gcagaaactg accggccttg agcatgaaaa actgctcgac gaatacaaag
     6301 agctgctgga tcagatcgca gaactgctgc gtattcttgg cagtgccgat cgtctgatgg
     6361 aagtgatccg cgaagagctg gaactggttc gtgaacagtt cggtgacaaa cgtcgtactg
     6421 aaattactgc caacagcgcc gacatcaacc tggaagatct gatcactcag gaagatgtgg
     6481 ttgtgacgct ctctcaccag ggctacgtga agtatcagcc gctttctgaa tacgaagcgc
     6541 agcgtcgtgg cgggaaaggc aaatctgccg cacgtattaa agaagaagac tttattgacc
     6601 gactgctggt ggcgaacacc cacgaccata ttctgtgctt ctccagccgt ggtcgcgtct
     6661 attcgatgaa agtctatcag ttgccggaag ccactcgtgg cgcgcgcggt cgtccgattg
     6721 tcaacctgct gccgctggag caggacgaac gtatcaccgc gatcctgccg gtgaccgagt
     6781 ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg tactgttaag aaaaccgtcc
     6841 tcaccgaatt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg gttgaaggcg
     6901 atgagctgat cggcgttgac ctgaccagcg gcgaagatga agtaatgctg ttctccgccg
     6961 aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc aacaccaccg
     7021 gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc gtgcctcgtg
     7081 gcgatggcgc aatcctcacc gcaacgcaga acggttatgg taaacgtacc gcagtggcgg
     7141 aatacccgac taagtcgcgt gcgacgaaag gggttatctc catcaaggtt accgaacgta
     7201 acggtttagt ggttggtgcg gtgcaggtag atgactgcga ccagatcatg atgatcaccg
     7261 atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtgggc cgtaacaccc
     7321 agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg caacgtgttg
     7381 ctgaaccggt tgacgaggaa gatctggata ccatcgacgg cagtgccgcg gaaggggacg
     7441 atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa ttttacttct
     7501 tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct gcaaagtctg
     7561 ggcttttgtc gtattagggc gcggtaaagt tttgctgtgc tcataaaaaa tggctggctt
     7621 tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg gttctatggt
     7681 cttgtaggtt tgggcgcaat agtactgctt tccgctggcg tctgggcgtt gcaatatgct
     7741 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     7801 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagacctc
     7861 cttaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     7921 catgccgatc gtctgggcat tgaaggcagc attcgccgca ttgtctatga acacgatctc
     7981 acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc ggcgctatgg
     8041 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     8101 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     8161 atcagtagta ttaagctaaa tagtgaaacc atcccggttt atcagttgcg ctataacggc
     8221 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagtacggat
     8281 atgctgttta aagatgacca gcaggatacc gaagccacgg cgatcgcaag tgatttgttg
     8341 agcggtaaaa aacgctggca agccagcttt ggcctggaag agcgtactgc cgaaaaaacg
     8401 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg ggtttggcta ccagcgatta
     8461 atgccttctt ttgctggcgt acgcttcgaa atgagcaacg acggctggca tagctttgtg
     8521 gcgttaaatg atgaatccgc aagcgtagat gccagtttcg attttacgcc ggtatggaac
     8581 agtatgcctg ccggggccag cttctgtgtg gcggtgccgt attcgcatgg tattgccgaa
     8641 gagatgcttt cgcacatcag ccaggaaaac gacaagctga acggggcgtt agatggtgca
     8701 gccgggctgt gctggtatga agactcaaaa ttacaaaccc cgctgtttgt cggtcagttt
     8761 gatggcactg ccgaacaggc acaactgcct gggaaactgt ttacgcaaaa tattggtgcg
     8821 cacgaaagca aagcgccaga aggtgttttg ccggtaaacc agactcagca gggcgaagcg
     8881 caaatctggc gtcgcgaagt gagttcccga tacggccagt atccgaaaac acaggcggcg
     8941 caaccagatc aactaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     9001 acgctgcttt tctccctcga tgacacgctg gttaataacg cgctgcaaac actgaataaa
     9061 acccgcccgg caatggtgga tgtaataccc actgacggca tcgttccgct ctatatcaat
     9121 ccacaaggcg tggcgaaact gctgcgtaac gaaacgctga ccagtctgcc gaagaatctc
     9181 gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga cgctttatct
     9241 caacaaccgc gttatgtcat gaagctggca cagatggaac ccgatgccgc ctggcagtgg
     9301 ctacccataa cctggcagcc gctatgaggc acggactgct ggcgctgatt tgctggctgt
     9361 gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca gtcggggctg tttcgcgcct
     9421 ggtttgtgcg tattgcacag gaacagctcc gacaggggcc aagtccgcgc tggtatcagc
     9481 aggattgtgc gggcctggtg cgattttcgg cgaacgaggc gctgaaagtt cacgacagca
     9541 aatggctaaa aagtaacggc atagcaagtc agtatttgcc gcctgaaatg acgctaacac
     9601 ctgaacagcg tcaactggcg caaaactgga atcaggggaa cggaaaaacc ggcccctatg
     9661 tgaccgcgat taatttgatt cagtacaaca gccagttcat tggccaggac ataaaccagg
     9721 cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta atggtctgga
     9781 tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac ggaatgcgcg
     9841 cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc aacgattcca
     9901 atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag cgtgcttgtc
     9961 cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt ccagcaacta
    10021 tgcgccgccc gccgggggaa cattcttttt gctcgctgac agcagtttta gcagcagtga
    10081 agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc agatggaaga
    10141 gtacggcggt gtggacgttc gtctgtatcg tattcctgac ccgatggcat ttttgcgcca
    10201 gcagaaaaat ctgcatcgca ttgtggttca accgcaatat ctgggcgacg ggctgaacaa
    10261 cacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtga tgcagcgtac
    10321 tttttcttct cagtctcggc agaatgtgac tcaggcgtta cccgaattac agctcggcaa
    10381 tgccattatt aaaccttccc gttatgtaca gaacagccag ttttcaccgc tgaaaaaata
    10441 tccactggtg gaacagttcc gttacccact atggcaggct aaaccggtcg agccgcagca
    10501 aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg gtaacatcta
    10561 tattcctctc ggcaaacaag agccgggact gtacctcgtc gaggcgatgg tcggtggata
    10621 tcgggcgacg acggtggtat ttgtctctga taccgtggcg cttagcaaag tgtcaggtaa
    10681 cgagctcctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagccgg gatcagagat
    10741 cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca gcggtacctt
    10801 gcagttacaa catatatcgc cggaacgttc atacattctg ggtaaagatg ctgaaggcgg
    10861 cgtttttgtc tccgagaatt ttttctacga aagcgaaatc tacaacaccc gcttgtatat
    10921 ctttaccgac cgccctctgt atcgtgcagg cgatcgtgtt gatgtaaaag tgatgggccg
    10981 cgagttccac gacccgttgc attcatcccc catcgtcagc gccccggcga agctttcagt
    11041 gctggacgcc aacggcagtc tgttgcaaac catcaatgtc acgctggatg cgcgcaatgg
    11101 cgggcagggg agtttccgcc tgccagaaaa tgccgtcgcc ggaggttatg agttacgcct
    11161 ggcttaccgc aaacaggtct atagcagcag ttttcgcgta gccaactaca tcaaaccaca
    11221 ttttgagatt ggcttagccc tcgacaaaaa agatttcaaa actggcgaag cggtcagcgg
    11281 caaactgcaa ctactctatc cggatggcga accggtaaaa gatgccagcg tgcagttgag
    11341 tttgcgcgct cagcaacttt cgatggttgg taacgatttg cgttatgccg gacgtttccc
    11401 ggtgtcgctg gaaggtagcg acgctgtttc tgacgataaa ggcaatgtag cgttaaacct
    11461 tcccgccgcc gataaaccga gccgctattt actgaccgtc tccgccagcg atggcgcggc
    11521 atatcgtgtt actactacca aagagatcct cattgaacgc ggtctggcgc attactctct
    11581 aagcacctcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct
    11641 ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag accgcacgag
    11701 ccatagcgga gagctaccgt caggcggcaa atcctttacc gtcaatttcg ataaacctgg
    11761 caactacaat ctgacattac gcgataaaga cggcttaatt ctcgctgggt taagtcatgc
    11821 cgtcagcggt aagggcagca cggcgcatac tggtacggta gatatcgtgg cagataaaac
    11881 gctgtaccag cctggcgaaa ccgcgaagat gctgattacc ttcccggagc caattgatga
    11941 agcattattg acgctggaac gcgatcgcgt tgaacagcag tcgctgcttt cgcatccggc
    12001 aaactggctg acgttacaac gtttaaacga tacccagtat gaagcccgcg taccggtgag
    12061 caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaacg gtcagtacag
    12121 ttttcagaac gccgggatca aagttgccat tccccagctg gatatccggg tgaaaacgga
    12181 caaaacccat taccagcctg gtgaactggt taatgtcgaa ttaacctcgt tgctgaaagg
    12241 taaacctgtt tctgcgcagc taacggttgg cgtgatcgat gaaatgatct acgcgctgca
    12301 accggaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta acaatgtgcg
    12361 taccagctcc agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg agccggttgc
    12421 gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc cacggcgtga
    12481 agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag gcaaagcgta
    12541 tttcacgttc ctgatgcctg attcgttaac ccgctggcgc attaccgcgc gtgggatgag
    12601 cggcgacggg ttggtcgggc aggggagtgc ttatctgcgt tcggaaaaaa atctctacat
    12661 gaagtggagt atgccaacgg tgtatcgcat gggcgacaaa ccggcagcag gactgtttat
    12721 cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag gcgctgagat
    12781 gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctgacgc agaacattca
    12841 gcaatctggc ttgttaagcg cagaactgca acaaaatggg caagtgcagg acagcattag
    12901 cacaaaactg tcttttgtgg ataacagctg gcccgttgaa cagcagaaaa atgtcatgct
    12961 cggcggtggc gataacgcgc tgatgttgcc cgagcaggcg agcaatatcc ggctacaaag
    13021 tagtgaaacg ccgcaggaga ttttccgtaa caatcttgat gcgttagtcg atgaaccgtg
    13081 gggtggcgtg atcaacaccg gtagccgtct gatcccgctc agtctcgcct ggcgttcgct
    13141 tgccgatcat caaagtgccg ccgctaacga cattcgtcag atgattcagg ataaccgtct
    13201 gcggctgatg caactggcgg ggcccggagc gcgctttacc tggtggggtg aagatggcaa
    13261 tggtgacgcc ttcctgacgg catgggcatg gtacgccgac tggcaggcca gccaggcgct
    13321 cggcgtaacg caacaaccgg aatactggca gcatatgctc gacagttatg ccgagcaggc
    13381 agataacatg ccgttattgc atcgggcgct ggtgctggcg tgggcacagg agatgaatct
    13441 gccgtgcaaa acgttgttga aagggttgga tgaagctatc gcccggcgcg gaactaaaac
    13501 tgaagatttc tctgaggaag acacccgcga tatcaatgac agcctgatcc tcgatacacc
    13561 ggaatctcaa ctggcagatg cggtggcaaa cgtcttaacc atgacgttgc tgaaaaaagc
    13621 gcagttgaag tccacggtga tgccaaaggt tcagcaatat gcgtgggata aagcggcaaa
    13681 cagcaatcag ccgctggcgc acacggttgt gctgctcaat agcggtggtg acgctaccca
    13741 ggcggccgct attttaagtg gtttgaccgc tgagcaatcc actattgagc gcgcgctggc
    13801 catgaactgg ctggcgaaat atatggcgac aatgcctccg gttgtgttgc ctgcgcctgc
    13861 gggcgcatgg gctaaacata agttaactgg agggggcgaa tactggcgtt gggttggtca
    13921 gggcgtgccg gacattctct cttttggtga cgaattatcg ccgcaaaatg tgcaggtccg
    13981 ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt ccggtgaccg ttgaacgcca
    14041 gttgtatcgg cttatccctg gtgaagaaga gatgagcttt actctgcaac cggtgaccag
    14101 caatgagatt gacagcgatg cgctgtatct cgatgaaatc acgcttacca gcgagcagga
    14161 tgcagttctg cgctacggtc aggtggaagt accgctccca ccgggagccg acgttgagcg
    14221 cacaacatgg ggcatttcgg tcaataaacc caacgccgcg aaacagcagg ggcaattgct
    14281 ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg gtgccggtga aagaactgac
    14341 gggaacggtc actttccgcc atttgctgcg cttctcgcaa aaagggcaat tcgttctgcc
    14401 tcctgctcgt tatgtgcgtt cctatgcacc tgcacagcaa agtgttgcgg cagggagtga
    14461 atggaccggg atgcaggtga aataagtgaa ctggcgcaga atcgtctggc tattggcgtt
    14521 ggttactctg ccaacgcttg cggaggagac gccgttacaa ctggtgctgc gcggtgcgca
    14581 gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc aaagcctcga cattacctga
    14641 ctcgctcacc acgccgttag ggagtttgtg gaaactgtat gtttacgcct ggctggagga
    14701 tacccatcaa ccggagcagc cctaccagtg tcgcggaaac tccccggaag aggtttattg
    14761 ttgtcaggct ggggagagta taacgcgcga taccgcgctg gtacgttcct gtgggctcta
    14821 ttttgcgccg cagcggttgc atattggcgc agatgtgtgg gggcaatact ggcaacagcg
    14881 tcaggcacct gcgtggttag cgtctcttac tacgctgaag ccagaaacct cggtaacagt
    14941 taaatcgctg cttgattcat tagcgacgtt acctgcgcaa aacaaagctc aggaagtctt
    15001 actggatgtg gtgctggacg aggcgaaaat cggtgtcgca tccatgctcg gtagtcgggt
    15061 acgggtgaaa acctggagct ggtttgccga tgataaacag gaaatccgtc agggtggttt
    15121 tgctggctgg ttaaccgatg gtacaccgct gtgggttact ggcagtggca ccagcaaaac
    15181 ggtactaacc cgttatgcga cggtgcttaa tcgggttttg ccagtgccga cgcaggttga
    15241 cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga aaatcacagc
    15301 ggaaaagagt acgacggcag taaaacccgg cgtacttaat gggcgctatc gtgtgacgtt
    15361 tactaatggt aaccacatta cgtttgtcag ccacggtgag acgaccttac tgagcgataa
    15421 gggcaaactg aaattgcagt cgcatctcga tcgtgaagag tacgttgcca gagttctgga
    15481 tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg cgattcgcac
    15541 ttttttacag caaaacgcca atcgggaagg cgattgcctg accatccccg acagcagcgc
    15601 cacgcagcgt gtttctgctt cgccagcgac cacgggggcg cgaacgatga ccgcctggac
    15661 gcaggatctc atctatgcag gcgatccggt tcattatcat ggcagtcgcg ctaccgaagg
    15721 gacactttcc tgggggcaag ccacggccca ggcggggcag ggagagcgtt acgaccagat
    15781 actcgcgttt gcttatcccg acaacagcct tagccgctgg ggagcgccac gctcaagctg
    15841 ccagttatta cccaaagcga aagcctggct ggcgaaaaaa atgccgcagt ggcggcgtat
    15901 attacaaggc gagacggggt acaacgaacc agacgtgttt gcggtctgtc gtttagtctc
    15961 aggtttcccc tataccgatc ggcagcagaa acggctgttt attcgtaact tctttacgct
    16021 tcaggatcgg ctcgatttaa cccatgagta tctgcacctg gcctttgatg gttatcccac
    16081 cgggcttgat gagaactata tcgaaacgct gacccgacaa ttattaatgg actaatgcta
    16141 tgcgaaagat ttttcttccg ttgttactgg tggcgctttc gcctgtcgct cacagtgaag
    16201 gtgtgcagga agtcgagatt gatgcaccgc tctctggctg gcatccggca gagggcgaag
    16261 atgccagttt ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg gcagatgatc
    16321 aaaatatctc agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg ggtaaagttc
    16381 agcagggccg gctggtggtc aacggtgcca gtatgccgca gagaattgaa tccgatggtt
    16441 catttgcacg cccttatatt ttcactgaag gcagcaacag cgtgcaggtc atcagccctg
    16501 atgggcaaag ccgacaaaaa atgcagtttt actcaacgcc gggcgctgga gtgatccgcg
    16561 cgcgtttacg gctggttctc tcctgggata cggacaatac cgacctcgat cttcacgttg
    16621 taacacccga tggcgaacac gcctggtacg gtaacaccgt gctgaaaaac agtggtgcac
    16681 tggatatgga tgtcacgacg gggtacggac ccgagatttt cgccatgcca gcgccgattc
    16741 acggacgtta tcaggtgtat atcaactact atggcggacg cagcgaaacg gaattgacga
    16801 ccgcccaact gacgctgatc accgatgaag gatcggtcaa tgagaaacag gaaactttca
    16861 ttgtaccgat gcgtaatgct ggcgagttga cgctggtgaa aagttttgac tggtgattaa
    16921 tggggttacc ttcgccgtcg atgttcaaga aaacgcccga taactttcgc tatcgggtgt
    16981 ttttattgat taattcaacc gttcaatcac catcgcaatt ccctggccgc caccaatgca
    17041 cagtgttgcc agccccagcg ttttatcgcg cgcctgcatg gcatgtaata gtgtgaccag
    17101 aatacgagca ccactggcac cgataggatg cccgagcgcg atggccccgc cgttgacatt
    17161 cactttctca gaatcaaagc ccagggtttt cccaacggca aggaactgtg cagcaaatgc
    17221 ttcattagcc tcaatgagat caatatccgc cagttgcagc cccgccagtt gtaacgcttt
    17281 ttgtgtggca ggtactggcc ccatacccat caatgcgggg ggcacgccac cgctggcata
    17341 acttttaatg cgagccaggg gggtaaggcc tgctgccagc gccgcagatt cttccataat
    17401 caccagagcg gcagcaccgt cgttaatacc agacgcgttc ccggcggtga ctgttcctgc
    17461 tttatcgaag gccgggcgca atgcgcctaa cgcttcagtc gtagaatccg ctttcgggaa
    17521 ttcgtcttga ctgaagacga aggttttctt ccgagtgaca acatttaccg ggacgatttc
    17581 ggctgtaaaa gcaccggact caattgcggc tgccgcttta cgctgtgaat gtagcgccag
    17641 ttcatcctgc atttctcggg taattccgta ctctttggcc acgttctcgg cggtaatccc
    17701 catatgataa ccatgggtgg cgcacatcag accatcgcgc aggattacgt cataaacctg
    17761 tccgtctcca agacgataac cagagcgtgc ttttgcatcg agtaagtagg gcgctaaact
    17821 catattttcc ataccccccg ccacaatgct ttgcgcctga cctgcctgaa ttgcctgggc
    17881 ggcaagcgcc acacttttaa gacccgaacc acacacttta ttgaccgtga atccgcacac
    17941 cgtttctgcc agcccgcttt ttaacagtgc ctgacgtgcc ggattttgcc ccaacccggc
    18001 ttgcaacacg ttacccataa tcacttcatc aatgtgtagt gaatcgattt ttgcacgttc
    18061 aatggcggct ttaattactg tcgcccccag gtcgatggcg ctggtggaag cgagtgaacc
    18121 gttaaaacta ccgatagcag tacgtaccgc actgacgatg acacaatttt tcatttttta
    18181 ttccttctgt ctgtagggtt gccgtcagaa cagcgttaaa ccaacgacga aaatgacgcc
    18241 ggagaagagc agggcagtga tgcaatagcc catgatgtcg cggacaccga gtccggcgat
    18301 tgccagtgct ggcagcgccc agaatggttg tgccatgttc atccattgct cgccgtaggc
    18361 gatcgccatt accgatttac cgagatcagc gcccagcgcc tgggctgcgg gtatcacgaa
    18421 aggtccctga ataacccagt gaccgccgcc agacggaacg gcgaagttaa tcagtgcaga
    18481 actaaaaaag gtcattaccg ggaaggtgtc tttgttcgca acattgatga agaattcggt
    18541 aatgagtccg cccagaccgg agtgctccat catcagttgg atcccagcgt agaaggggaa
    18601 ttgcaccaga ataccggcag tactgcgtgc tgccgcgctg atagcacgca tataagccat
    18661 tggcgtttta tgtagcagca gacccgcaat cataaacatc aggttgacgg tattgatggt
    18721 gatgttgaag ccatgttcgc tgaagtacat cgcaaggtaa gcgataccga gtgcgccgat
    18781 gatcaacgtc agaatgcggc tttcttccag tcgctcggat ggtggggcat ctttcggtag
    18841 ctgcttttgg aaatcagcct cttccatgag tagtttcgga tcgatactca ccacgtcaga
    18901 cggttttggc atcatcatgc gggtgataaa tggcatcacc acaatcaacg ccacagtgat
    18961 gaaaatgtta aaaccactga acagagtatc gcccaccggg atcagcccgg cgatatgctc
    19021 aaccgggttg cccggtgttg cagccaacag aggcattgag ccagagaaac cgccacccca
    19081 ggtgagaaaa ccaatgtagg cgcaggcaat gagcaacgga taatcagaac cgggtactcg
    19141 gcgggcgact tcacgggcaa acattgcgcc gacaaccaaa ccaaatcccc agttgatgac
    19201 acaagcgact gaaccgaaga aagtaaccag catgacgccc tgtacgggcg tctttgcggc
    19261 ggaggcggca gtacgcagca aactttttac cggagcagag ctggcaaggg catgaccggt
    19321 aacgatgata agcgccatct gcataccaaa cgccagcaag ttccagaaac cgtcacccca
    19381 cattttcacc atgctgatcg gcgtttgtgg tgttaaccaa agcgcgatca cgaaagttag
    19441 caatgtcagc aacatggcaa agatcagtgg atcgggaagc caccggctga caaaacgcgt
    19501 cataaaacgc gatatgcgac caatcataaa tcaccccgtt gcgtattcag atcggcggca
    19561 acttcaaacc gagcttctgt tttggcacac acggtggcta aatcacaccc gtcggcaatt
    19621 tcggtgagcc acattttgcc gtcaataaaa cgaaagacag ccagttcagt aaccagcata
    19681 tgcaccgcat gttgcgcagt gagtggcatg gtgcagcggc gcaaaatttt tgctgaacca
    19741 tctttggcac aatgttccat ggcgatgatc actttgcgcg acccggtcac cagatccatc
    19801 gcgccaccca taccgggcac cattttccca ggcactaccc agttcgcgag gtttgcttct
    19861 tcgtctactt gcaaaccgcc gagcacgcag gcatcaatat gaccgccgcg gattagcgca
    19921 aatgacatgg cgctatcaaa catggctgca ccgggtaaaa caccgcacgg ttgtccgcca
    19981 gcgttcacca gatctggatg cgctgtcgtg accgggccta aaccgaggaa gccgttttcc
    20041 gattgcagag tgatatgaat accctccggt aaataattgg cgaccattgt gggtaaaccg
    20101 atccctaagt taacgatgtc accatcacga agctcttgcg ccacacggcg cgcaatacgt
    20161 tgtttcgcat ccattatttg ctctcctgtg aaacgatgat gtggtcgata acggcaccag
    20221 gggtgacaat atggtcaggt tgcagctcgc cggtttcgac cagttcatct ggctctacca
    20281 gcgtgatatc agccgcaagg gctatcaggg ggttaaagtt gcgggcgcta agttgatagg
    20341 tcaggttgcc aagtgtgtcg caacgatgag cgcgaattag cgccaggtcg gcgcgcagtg
    20401 ggcgttcgag cagccaggtt ttaccgtcga gtgtcagtgt ctgtttgcct tcctctacga
    20461 cggtgccgac acccgttggg gtgagaaaac caccaagtcc agctccgcca cagcgaattt
    20521 gctcgattag cgtaccttgc ggcaccagaa cgacgtccat ctcaccagat atcatgcgcc
    20581 gacctgtttc cgggttggtg ccgatatgtg aagcaatcac tttgcggact cgaccattga
    20641 cgatgagcgg accgatgccg gtatcaacaa acgcggtatc attggctatc aatgtcaggt
    20701 cgcgaacacc tgattccagt aatgcttcaa ccaggcagga tggagtgcca atccccataa
    20761 atccgcccac catgatggtc atgccgtcac gaaagaagcc ggtggcatct tgtaatgtca
    20821 tcaattttgt tttcattttt tatccctctt gcatacaccg ttgtgggtac agtcagggaa
    20881 tagcaagggg agtgccagaa tgagattatt gggttttgtt atttaactaa ttgaatatga
    20941 agggataaat tggatgagga tttataagga gaatgagcgg ctatgcagaa aattgcgcac
    21001 tgtgcaaatt tctgcatagc aagttttggc gttatacatc cgccggatcg ataccgtatt
    21061 cctggagttt atacatcaat gcacggcgac tgatgcccag cattaaagca gtgcgggttc
    21121 ggtttccttc ttgttgttcc agcacttcca taatgatgcg tttttcgacg cgtttaattt
    21181 cctcttttaa attacgctct ccgacagggg atgtttttac ctcgccagca ttacagactg
    21241 gctgacgaat ctgcggcgga agatcctcag aaaaaatgat cgggcctgag ttcatcacga
    21301 cggcgcgttc aataacgttg gaaagctctc gaatatttcc cggccatgac caggcggtaa
    21361 gcagtgacat tgccatcgga tcgatgtcga taatatcgcg ctgattctca ctactgaatt
    21421 tttgcaaaaa gtgattagct aacagggaaa tatcttcccg gcgatcgcgc agaggcggca
    21481 gtattaaatg aataacgtta aggcgataaa agagatcttc acggaaggtg ccttctttta
    21541 ccattgcctg caagtcacgg ttggtggcag caatgatgcg gatatcaact tttatggtct
    21601 gatggccgcc aatccgttca aattcccgtt cctgcagaat gcgtagtaat ttggcttgca
    21661 gtacgagcgg catttcgcca atttcgtcga ggagcagagt accttcgttg gcacgttcaa
    21721 ataatccctg acgcaaggtt tgtgcaccag taaatgcacc tttttcatga ccaaacagtt
    21781 cactttcgag caacgattcc ggcagcgccg cgcagttgac tttaatgaac ggcccctttg
    21841 cccgccgcga attgtagtga atcgctctgg caatcaactc tttcccggtg ccgctttcac
    21901 cgctaatcaa gacgctggcc tgagaaaggg caattttggc ggtgtctttg cagatgtcca
    21961 tcatcgccgg gctgttggtg agaatgtgcc cccattgcca gctagtgctc agtgcctggt
    22021 gcagatggcg gatctctttt ttcattgact ggagttgtaa agcgcgctga acgattaaat
    22081 tcaactcatc gagatcaaac ggtttgataa catagtcgaa ggccccgcag cgtagcgctt
    22141 cgacggcggt ttccacttca gcataggccg tcatcagaat aacgggggtc cgtgtctcat
    22201 ggctgcgcat ctccttgagt gccttgatgc cgtccatctc tggcatgcgg atatccatca
    22261 acaccacgtc agggtgaata tcagcaaaca ggtgtaatgc tgtgcgtccg ttgttcgcac
    22321 aatgtgtttc gaaaccttgt agtgcaaaag cggtgctcag catacggcga acattatctt
    22381 catcatccac aataaggatg cgattaatag cagtcataca gtctgatttc cctgcgggtt
    22441 gatcggtaaa ataagcgtga aggttgcgcc gtagcccggc aaactggcga cgcgaatatc
    22501 accctgatgg gcattaatga tgcgttgact taacgccaga ccaagcccgg ttcctgaggc
    22561 tttggtggtg aaaaagggat cgaagatctt tttttgcagc gagagatcaa tgccacagcc
    22621 gttgtcctct atcgaaatgg cctgttgtga gtcgctgtat tgccaggttt gaatgcgaat
    22681 tttccctcgt gcgctgatag cctggacggc attgatcagg atattcagta gtacctgttt
    22741 gagcagttca cgatcggcgt taatcgggtt taattcatta tccagttcgc ttatgaagtc
    22801 gacccgcgct tgtacgccgg cggtttgtac cagtaccaga gtttcttcaa ccaatgcatt
    22861 gagactgact tgttgccatt gactgtggcg tggacgtgaa aattcgagca attgctgaat
    22921 aactttgtta attgaatcga tttctttgag tactacggac agatattcct gatgtattag
    22981 gtcgctggtt tgttggcgca agatctgtac ataaccacga atagccgtta gcggattacg
    23041 tacttcatgc gcgacgccag ccatcagctc acccagtgtg gcgaggcgtt ctgcttgcgc
    23101 catgcggcgc tgggtttctt tgcgggcagt taaatcagag aaaatcacca aagcacctat
    23161 catttcacca tgcgtgttat gaatacgact ggtagtgaca ctgagttcaa tggtgcggtc
    23221 acggcctgga aaactgatct ccagcgctac atgttcggtg ccatgttcca gcgtatccag
    23281 tactggactg tagaactgag tattgtcgaa caacatggag taaggctgcc ctaccagttc
    23341 atgacgttga tagccagtga tgacttctgc tgccgggttc atggtggtta catcaccctg
    23401 gcggtcaatg gcaatgacgc catcggcagc gttttcaata atcagatcgt taagtgtccg
    23461 cgtttcacgc agtgcctggg cgaggttgtt aacactctga ctgatttgcc ccatttcacc
    23521 gggcaattgt ggtaatcgag tgggaatatt ttgcgccaga gtcgagaggc catcggtgat
    23581 gatatcaata ttggcgctca ggcgacggga gaaaaggaca atcaacagca ggcttatcag
    23641 caaaccggcg gtgagcacaa tgataatcct cacatccatt ttccaggcct ggcggcgaat
    23701 atcttcggtt aattcattgg cccagatata gccgaggatt tcaccattac gctcaatggg
    23761 gatcattgaa ttcaaaatat cgccgcgcac ctgcctgcct gaataaacca aaggggtatt
    23821 tgtacgcatg acttcgcgac cagggtgatc tgcggcaatg gtaacgccga cattattctg
    23881 atatagcgct gaaggcgcgt aggtgattat cgcatccagt gttttgttgt aataaccagc
    23941 gccgatgcca gggaaggcgt gagtgatatt ttcggtaatg ggggcaagtt ctgcatttaa
    24001 tgcgcggata cgctcctcac gtggtaagtc gatgtagaga tcatagcgat cgcctagtgc
    24061 ctgattaagc aggttgacca cggcagaaag ttttttctct ttttcagata agactgctga
    24121 acgtccttct gtttctacga tataaccaat agtaagcgtt gggacaatga ccatcaggat
    24181 tgccatcagg atcatttgat tgcgtaagcg gcgtggataa atccacttca tataatgcat
    24241 gctgaaatcc actagtcttg tccggtatat gacgattatc agaggttaag gtgatgattt
    24301 ctcggcggtg tatcatattc cagagaagag agaacattgc ggtaacacgc ttttaccgct
    24361 accttaacca cactccatcg gtcacctgag gcggagcttc gcccctttga aataccttgc
    24421 ttcttttcgt acaaccctga aagcctcgcg ctacatgttc agagcattgg cgttagtgct
    24481 ctggctgttg attgcttttt catccgtttt ttacatcgtt aatgcgttac atcagcgaga
    24541 atcggaaatt cgtcaggaat ttaatctgag ttccgatcag gctcagcgct ttattcaacg
    24601 tacctctgat gtgatgaaag agctgaagta catcgctgaa aatcgcttat cggcagaaaa
    24661 cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat gtgcctgcgt ttgaaccgct
    24721 gtttgccgac tccgattgtt ccgcaatgag taacacctgg cgaggctctc tggagtcatt
    24781 ggcgtggttt atgcgctact ggcgcgataa tttctctgcg gcttacgatc tcaaccgggt
    24841 atttttaatc ggcagcgata acctctgcat ggccaatttc ggtctgcgtg atatgccagt
    24901 ggaacgcgat accgcgttga aagcgttgca tgaacgcatt aataaatatc gaaatgcacc
    24961 acaagatgat agtggcagta acctctactg gatcagcgaa ggtccgcgcc ctggcgtcgg
    25021 gtatttttac gcgttgacgc cagtttatct ggcgaaccgg ttgcaggcgc ttttgggtgt
    25081 cgagcagact atccggatgg agaatttttt cttacctggt acgttgccga tgggggttac
    25141 cattcttgat gaaaatggtc ataccctgat ttcgcttacc ggaccagaaa gcaaaattaa
    25201 gggcgatcct cgctggatgc aggaacgctc ctggtttggc tatacggaag ggttccggga
    25261 gctggtgctg aagaaaaatc tgccaccctc atcgctaagc atcgtgtatt cggtgccggt
    25321 tgataaggtg ctggaacgca ttcgcatgtt gatccttaac gcaattttgc tgaatgtgct
    25381 tgccggagct gcattgttta ctctcgcgcg gatgtacgag cgacgtattt tcattccggc
    25441 ggaaagcgac gccctgcgac tggaagaaca tgagcagttt aatcgcaaga ttgtcgcctc
    25501 cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc gtcaatattt taagtaacga
    25561 actggcgcat acctatctca atatgcttac gcatgaggat cgccaacgac tgacgcaaat
    25621 tatctgtggg cagcaggtca attttgttga tgtcctgacc agcaacaata ccaatctgca
    25681 aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg gccatttgtg tgctggtgga
    25741 tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag atggcacaag cagcggaaca
    25801 ggcgagccag tcaaaatcga tgttccttgc caccgtcagt catgagctgc gaacgccgct
    25861 gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa gagttaccga aaggcgtcga
    25921 tcgtctggtg acggcaatga acaactcttc cagcctgttg ttgaaaatta tcagcgatat
    25981 tctcgatttc tcgaagattg aatcggaaca gttgaagatc gaaccgcgtg agttttcacc
    26041 gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg ctggtggtac gcaagcagtt
    26101 aggcttgtac tgctttattg aaccggatgt gccagtggcc ttaaatggcg acccgatgcg
    26161 tttacagcag gtcatctcca acctgttgag taacgccata aaattcaccg ataccggctg
    26221 tatagttttg catgttcgca ctgatggcga ttatctctct atccgtgttc gcgataccgg
    26281 cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat cccttcttcc aggtcggaac
    26341 gggcgtacag cgtaacttcc aggggaccgg tctgggtctg gcgatttgtg aaaaactgat
    26401 cagcatgatg gacggcgata tctcggtaga ttcagaaccg ggaatgggta gccagtttac
    26461 cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa aaaggcgtgg aagggttgag
    26521 tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc tgtcagttcc tggaaaccag
    26581 tttgcagcgc agcggcatcg tcgttacaac atacgaaggg caggaaccga ctcccgaaga
    26641 tgtgttaatt actgacgagg tagtgagtaa aaaatggcag ggtagagcgg tagtgacctt
    26701 ctgtcgtcgc catattggta ttccgctgga gaaagcgcca ggggagtggg tacacagtgt
    26761 ggctgccccg catgagctac cggcattgtt ggcgcgtatt tatttgatcg agatggagag
    26821 cgacgatcct gctaacgctc tgccgttaac ggacaaagcg gtcagcgata acgacgatat
    26881 gatgattctg gtcgtggatg atcatccgat taaccggcgt cttctggcag atcagttggg
    26941 atcgttgggc tatcaatgta aaaccgcgaa tgatggcatc gatgcgctta atgtacttag
    27001 caagaatcat attgatatcg tgcttagcga tgtcaacatg ccaaatatgg atggttaccg
    27061 cttgacgcaa cgcattcgtc agttgggact gacgttgccg gtaatcggag taactgctaa
    27121 tgcgttggct gaagagaagc agcggtgtct ggagtctggt atggacagct gcctgtcgaa
    27181 gccggtaacg ctggatgtga taaaacagac gctaacggta tatgccgaga gggtcaggaa
    27241 atcgcgggat tcgtaggacg gataaggcgt ttacgccgca tctggcattc agtgcaaatg
    27301 ccagatgcga cgctgacgcg tcttatctgg cctacagatg attagtcttt atctgccgga
    27361 cttaaggtca cagaagagag ataattcagc agagcaatat cgttctcgac acccagcttc
    27421 atcatcgcag atttcttctg gctactgatg gttttgatac tgcggttcag ctttttagcg
    27481 atctcggtca ccaggaagcc ttcagcaaac aggcgcagaa cttcactctc ttttggcgag
    27541 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    27601 ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    27661 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    27721 ttgttgttca tggtcagaac gatgatcgac aggcttggga aatggcgctt gatgtacttg
    27781 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    27841 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    27901 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    27961 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    28021 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    28081 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    28141 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    28201 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    28261 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    28321 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    28381 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    28441 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    28501 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    28561 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    28621 ttcttcctct tcaacttccg ggtcagctgc gagcattttg atatgcacag agtagcgtgt
    28681 accaagccca tcccgcgttt taatgtttaa atggccgccc agtttacgtg ccagttgatc
    28741 gctcagccag aatgccagcg ggtccgcctt gccatagcga tcgttttggg tctggttgat
    28801 aaacgggaag tgcaaattat ccatttcatg aatgcttacg ccttctccgg tgtccagaat
    28861 gcggaacgtc aggcggtctt cggaggactc atcctgatca acctcaaggg tgatttttcc
    28921 caattgtgtt gaggtcacgg catattgcat cagtagcagc aaaatgcgtc gtaaggcatc
    28981 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    29041 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaag cctgcacgga
    29101 gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta actgtatttc
    29161 atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcgtt
    29221 gagtttagcc gcgctctctg ccagggactg tgcgggttct ttcagcgcat cgccaatgtt
    29281 tttcataaag gtcatccgcc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    29341 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    29401 cgcaacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    29461 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    29521 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    29581 gccgagcggc agcagtgaga ctatctcttc attgattgcc cgtaaaatgc gcaattcgtt
    29641 attgaccgcc gtgttgggta gactttctgt actgcgactg gagaaatggc ggaatgtggt
    29701 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    29761 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    29821 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    29881 cgtcccttct ttctcattat cattgtttcc cgttgccgtc gcgtctggct caaggcggaa
    29941 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacaac
    30001 cgttgccaga tgtcccggct ggttaaaggt ggtacgcaag gtaaagtaat gaccgttctg
    30061 ccaggccagg cggcgcatgt tagaaaagct ttcgcgttca tcgagggcgt tggcctgttg
    30121 caacatctct gcacgacgtg aatcaacaat gtcgctgacg gtcgattctt taaatccgga
    30181 ggtgagatct ttgaggggca gggttgagat cagcaccaga ctgttatcct gaccattcag
    30241 gtaatacatc gaccacggta cattttctgc gccccacaat gtatccagat aagtggacat
    30301 ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    30361 gcggcgcggt ttttccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    30421 gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg
    30481 ccaggtatcg acgcgctttt ggattgcgtg ggtaatgtcg acaatctggt aacttttatc
    30541 tttcagccag gcgttaacgg cgctttgtac cattactccc atcgtcacca gtaacacaat
    30601 gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc
    30661 cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg
    30721 gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga ttctctattc
    30781 tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc attttgctgt
    30841 acaaataacc tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa
    30901 ttatgctaat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt tctgtggtag
    30961 cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt
    31021 tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg
    31081 atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa
    31141 tacttaagaa taagttattg attttaccct tgaattatta ttgcttgatg ttaggtgctt
    31201 atttcgccat tccgcaataa tcttaaaaag ttcccttgta tttacatttt gaaacatcta
    31261 tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca
    31321 tttttgggga gaatggacct gccgactgat taatgagggt taatcagtat gcagtggcat
    31381 aaaaaagcaa ataaaggcat ataacagagg gttaataaca tgaaagttaa agtactgtcc
    31441 ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg ctgctgaaat ttacaacaaa
    31501 gacggcaaca aattagatct gtacggtaaa gttgacggcc tgcactattt ctccgataac
    31561 gactctaaag atggcgacaa gacctacatg cgtcttggct tcaaaggtga aactcaggtt
    31621 actgatcaac tgaccggtta tggtcagtgg gaatatcaga ttcaaggtaa cgaacctgaa
    31681 agtgacaata gttcctggac ccgtgtggca ttcgctggtc tgaaattcca ggacgttggt
    31741 tcttttgact atggtcgtaa ctacggcgtt gtttacgatg ttacttcctg gaccgacgta
    31801 ctgccagaat tcggtggtga tacctacgat tctgataact tcatgcagca gcgtggtaac
    31861 ggtttcgcga cctaccgtaa caccgacttc ttcggtctgg ttgacggtct tgattttgct
    31921 gttcagtatc agggtaaaaa cggtagtgct catggcgaag gtatgaccac taatggccgt
    31981 gatgatgtat ttgagcagaa cggtgacggt gttggtggtt ctattactta caattatgaa
    32041 ggctttggta tcggtgctgc agtttccagc tccaaacgta cctgggatca gaacaacact
    32101 ggcctgatcg gtactggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct
    32161 aacaacatct acctggctgc tcagtacacc cagacctaca acgcaactcg cgtaggttcc
    32221 ctgggttggg cgaacaaagc acagaacttc gaagctgttg ctcagtacca gttcgacttc
    32281 ggtctgcgtc cgtccctggc ttacctgcag tctaaaggta aaaacctggg tcgtggctac
    32341 gacgacgaag atatcctgaa atatgttgat gttggtgcga cctactactt caacaaaaac
    32401 atgtccacct acgttgacta caaaatcaac ctgctggacg acaaccgctt cacccgtgac
    32461 gctggcatca acactgatga catcgtagct ctgggtctgg tttaccagtt ctaatctcga
    32521 ttgatatcga acaaagggcc tgcgggccct tttttcattg ttttcagcgt acaaacacag
    32581 ttttttggtg tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt
    32641 acccgcgtgg cactgctggc tgccgcgctc ttctttgttg gttgcgatca aaaaacacaa
    32701 cccgccaaaa cccacgctac tgaagttacc gttcttgaag gcaaaaccat gggtaccttc
    32761 tggcgtgcca gcattccggg cattgacgcc aaacgcagtg ccgaacttaa agaaaagatt
    32821 cagacccagc tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg
    32881 atgcgattta acgactcgca aagtttatcg ccgtggccgg taagtgaagc gatggccgat
    32941 atcgtcacca cctcgctgcg cattggcgca aggaccgatg gtgcgatgga tataaccgtc
    33001 gggccgctgg tgaatctgtg gggctttggc ccggaacaac agccggttca aattccgagc
    33061 caggaacaga tcgatgcgat gaaagccaaa accggcttac agcacctgtc ggtcattaat
    33121 cagtcgcatc agcaatatct gcaaaaagac ctgccggatt tatatgtcga tctctccact
    33181 gtcggtgaag gttatgcggc ggatcacctg gcacgcttga tggagcagga agggatttcc
    33241 cgctatctgg tatcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgaaggccag
    33301 ccgtggcggg tagcgattca aaaaccaact gataaagaaa atgcggttca ggccgtggtg
    33361 gatatcaacg gtcatggcat tagcacctct ggcagctacc gtaactatta cgaactggac
    33421 ggcaaacgtc tttcccatgt tatcgatccg caaaccgggc gtcccatcga acacaatctg
    33481 gtatccgtga cggtgattgc tccgacggcg ctggaagccg atgcctggga tactggcttg
    33541 atggtactcg ggccggagaa agccaaagag gttgttcgcc gggaagggct ggcggtttat
    33601 atgatcacca aagaagacga tagctttaaa acctggatgt caccacagtt taaaagcttc
    33661 cttatcagcg aaaaaaatta aagcgcaaga ttgttggttt ttgcgcgatg gtgaccgggc
    33721 agactaaagg ctatccttaa ccagggagct gattatgaaa aacaccacat gtttaactga
    33781 cgatcaacgc tggcaatctg tcttagcccg cgacccgaac accgacggcg aattcgtttt
    33841 cgccgtgcgc accacgggta tcttttgccg tccgtcttgc cgcgccagac atgccttgcg
    33901 ggaaaatgtc tccttctacg caaatgccag cgaggcactc gccgccggat ttcgcccctg
    33961 caaacgttgt cagccagaca aagccaatcc ccggcaacat cgccttgata aaattaccca
    34021 cgcctgccga cttctggaac aggaaacgcc tgtaacgctg gaagccttag ccgaccaggt
    34081 ggcgatgagt ccgttccatc tgcatcggtt gtttaaagcg actaccggaa tgacgccgaa
    34141 agcctggcaa cagacctggc gcgctcgtcg tttgcgcgag tcgttagcga aaggggagag
    34201 cgtgacgacg tctattctta acgccggatt ccccgacagc agcagttact accgcaaagc
    34261 tgatgaaacg ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt
    34321 gcgttacgcg ctggctgatt gtgagctggg tcgttgcctg gtggcagaaa gcgagcgggg
    34381 gatttgcgcg atattgctgg gcgatgatga cgcgacacta atcagcgagt tgcagcagat
    34441 gtttcccgct gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt
    34501 gatcgccagc ctcaatcaac gcgatacgcc gctgacgtta ccgctggaca ttcacggcac
    34561 tgcttttcag caacaagtct ggcaggcact gcgcacgata ccttgcggtg aaaccgtcag
    34621 ttatcagcaa ctggctaacg ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc
    34681 ctgtgccgcc aacaagctcg ctatcgttat accttgtcat cgggtggtcc gtggtgatgg
    34741 cacactttcc ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc
    34801 tgagaatgag gagaggtaat gttggatctg tttgccgatg ctgaaccgtg gcaagagcca
    34861 ctggcggctg gtgcggtaat tttacggcgt tttgctttta acgctgcgga gcaactgatc
    34921 cgcgatatta atgatgttgc cagccagtcg ccgtttcgcc agatggtcac tccaggcgga
    34981 tataccatgt cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa
    35041 ggttatctct attcgcccat tgatccgcaa acaaataaac cgtggcccgc catgccgcag
    35101 agttttcata atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca
    35161 gatgcttgtc ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa
    35221 gacgaaccgg atctgcgcgc gccgattgtt tctgtttctc tgggcttacc cgcgattttt
    35281 caatttggcg gcctgaaacg aaatgatccg ctaaaacgtt tgttgttgga acatggcgat
    35341 gtggtggtat ggggcggtga atcgcggctg ttttatcatg gcattcaacc gttgaaagcg
    35401 gggtttcatc cactcaccac cgactgccgc tacaacctga cattccgtca ggcaggtaaa
    35461 aaagaataaa aataagaatt attattgctg tgtgcgcgaa gatgtttaaa ctgcgggctg
    35521 caattcattg tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc
    35581 tggccattta tcagtgtgat ggcgctaagc ctcgccagtg cggcattagg cattggctta
    35641 attgctttta tcaatcagcg ccttatcgaa acggcggata ccagtctgct ggtgttgccg
    35701 gagtttctgg gattattgct gctgttgatg gcagtcactc tcggatcgca actggcgctc
    35761 accactttgg ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctg
    35821 gatacccatg ttgagcgcat tgaacaactt ggtagcgcct cgttgttggc ggggttaacc
    35881 agcgatgtgc gcaatatcac cattgctttt gtgcgtctgc cggaactggt gcaggggatc
    35941 attctcacta tcggttcagc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta
    36001 acggctatct ggatggcgat caccatctgg ggcggttttg tgctggtggc gcgggtgtac
    36061 aaacatatgg cgaccctgcg tgaaaccgaa gataagctgt acacggattt tcaaaccgta
    36121 ctcgaagggc gcaaagagct gactctgaac cgtgaacgcg ccgagtatgt gtttaacaac
    36181 ctctacactc ctgatgcgca agagtatcgc catcatatta tccgcgcaga caccttccat
    36241 cttagtgccg tgaactggtc aaacatcatg atgctgggcg caatcggcct ggtgttctgg
    36301 atggcgaaca gtctcggttg ggctgatacc aacgttgccg cgacctattc gttgacgctg
    36361 ttattcctgc gtacgccgct gctttcggcg gttggcgcat taccgacgct gctgacggcg
    36421 caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg
    36481 cgcccgcagg cgtttcccaa ctggcaaacg ctggagctgc gtaacgtgac gtttgcttat
    36541 caggataacg cgttttccgt tggtccgatt aatctcacca tcaaacgtgg cgagctgctg
    36601 tttctgattg gcggcaacgg tagcggaaaa tcgacgctgg cgatgttgct gacgggcttg
    36661 tatcagccac aaagcggcga aatcttgctg gatggcaacc ctgtcagcgg cgaacaaccg
    36721 gaagattatc gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg
    36781 ctggggccgg aggggcaacc cgctaacccg caactggttg agaagtggct ggcgcagctg
    36841 aaaatggctc ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa
    36901 gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg
    36961 ctggatgaat gggcggcgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg
    37021 ctgccgctga tgcaggagat gggtaaaact attttcgcca tcagtcatga tgatcattac
    37081 tttatccacg ccgaccgcct gctggagatg cgcaacgggc aacttagcga gctgacgggc
    37141 gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct
    37201 gacctggcgg actgctgccg ccgggtcaat atgctaaaaa tcccttcatt tctaataacc
    37261 ctataattaa tcaacgaaat tataatgttt ctaaaattag aatataattt ataaacatta
    37321 tttaaatgtt gttgcttaag tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag
    37381 cgactaaaag taaggcatta acaagatgaa aaaagtgact gccatgctct tctcgatggc
    37441 cgtggggctt aatgccgttt cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac
    37501 tgatgtactg ttgattggcg gcggcattat gagcgccacg ttggggacct atttacgcga
    37561 gctggagcct gaatggtcga tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag
    37621 ttcgaacggc tggaacaacg ccggaaccgg gcattctgca ctgatggaac tcaattacac
    37681 tccgcaaaac gccgatggca gcatcagtat tgaaaaagca gtcgccatta acgaagcatt
    37741 ccagatttcc cgccagtttt gggcacacca ggtcgaacgt ggtgtgctgc gaaccccacg
    37801 ttcatttatt aataccgttc cacatatgag ctttgtctgg ggcgaggata acgtcaattt
    37861 cctgcgcgcc cgttacgccg cgttgcaaca aagctcgctg tttcgcggta tgcgttactc
    37921 tgaagatcac gcgcagatca aagagtgggc accgttagtg atggaagggc gcgatccgca
    37981 acagaaagtg gcagccacgc gtacggaaat tggtactgat gtgaactatg gcgagatcac
    38041 ccgccagtta attgcttcct tgcagaagaa atctaatttt tcgctgcaac tcagcagcga
    38101 agtccgcgcc ctaaagcgta atgacgataa cacctggacc gtcactgttg ccgatctgaa
    38161 aaacggcact gcacagaaca tccgcgccaa atttgtcttt atcggcgcgg gcggtgcggc
    38221 gctgaagttg ttacaggaat cggggattcc ggaagcgaaa gactacgccg gtttcccggt
    38281 gggcggtcag ttccttgttt cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt
    38341 ttacggtaaa gcatccgttg gcgcaccacc gatgtcggtt ccgcatatcg atacccgtgt
    38401 tctggacggt aaacgcgtcg tgctgtttgg gccatttgcc accttctcaa ccaaattcct
    38461 caaaaacggt tcattgtggg atctgatgag ttccaccacc acctctaacg tgatgccgat
    38521 gatgcacgtc gggctggata atttcgatct ggttaaatat ctggtgagtc aggtgatgtt
    38581 gagtgaagag gatcgttttg aagcgttgaa agagtactat ccgcaggcga aaaaagagga
    38641 ctggcgtttg tggcaagcgg ggcagcgcgt gcagattatc aagcgtgatg cagagaaagg
    38701 tggcgtactg cgtctgggta ccgaagtcgt cagtgaccag caagggacta ttgccgcgct
    38761 gctgggggca tcgccaggag cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa
    38821 agtatttggt gatcgcgttt ccagcccgca atggcaggct acgttgaaag cgatcgttcc
    38881 gtcttatgga cgcaagctga acggtgatgt agcggcaacg gagcgcgagt tgcagtacac
    38941 cagcgaagtg ctggggctga aatacgacaa gccgcaggca gcagatagta cgccgaaacc
    39001 gcagttgaaa ccgcaacccg ttcaaaaaga agtggcggat attgcgttgt gatgatgtgc
    39061 cacatccggc atggtatgcc ggatgtggcg tatgcagata agacgcgtca gcgtcgcatc
    39121 agtcaaccgg ctcgggcgtt agatgtcgga tgcggcgtga ccgccttatc cgacctacgg
    39181 ggtgccgcac ttcacagcag tttagcgaac taccgcgttg tcaattttct cttccgcctt
    39241 ccagacgcgg tacttcacat ccacattgtc tggcgtatac accacgatcg gcagcttgct
    39301 gttgtaacgc agcattccag catcgcccag atacgcggtg acaaatttct tctctttttt
    39361 gccatccggg caggccatca tcgtcgaaac cggcgagcta actttatcaa agacataata
    39421 atcatagccc cagccttcca gcgttttgct ttccagcttc ccgccgagac gatgcaaatt
    39481 gcaatcgact tccagcgtct gaccgattaa cagttctact ttcagggtag attcatcttc
    39541 ttgcggggtt aactgaatca cctggcgctt catgcctttt tcagcttgtg gataaggcgc
    39601 gattttttcc agtggctgga cgctttctgc cgcccaggcg gaagtggtgg cgaaagcggc
    39661 aaacaatact gcaggtagaa tggtcttcat ttttttgctt tccttcttca
//
LOCUS       DARNVM010000020        39526 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.020, whole genome shotgun sequence.
ACCESSION   DARNVM010000020 DARNVM010000000
VERSION     DARNVM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.020"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(130..312)
                     /locus_tag="SI621_001032"
     CDS             complement(130..312)
                     /locus_tag="SI621_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063774.1"
                     /translation="MPCNTVNPDIFYCSIFNRLQEYLVKGFVMDDERLKNPPVGSSVL
                     NASAICAPACAVFICR"
     gene            complement(337..431)
                     /locus_tag="SI621_001033"
     tRNA            complement(337..431)
                     /locus_tag="SI621_001033"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Sec,seq:tca)
     gene            724..2106
                     /locus_tag="SI621_001034"
     CDS             724..2106
                     /locus_tag="SI621_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HEI4063775.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQNVQN"
     gene            2116..4434
                     /gene="yicI"
                     /locus_tag="SI621_001035"
     CDS             2116..4434
                     /gene="yicI"
                     /locus_tag="SI621_001035"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEI4063776.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWK
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTESG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            4729..6282
                     /locus_tag="SI621_001036"
     CDS             4729..6282
                     /locus_tag="SI621_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEI4063777.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLEQWRITVEKRAGAGVMLDSKNITDLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            6309..6779
                     /locus_tag="SI621_001037"
     CDS             6309..6779
                     /locus_tag="SI621_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEI4063778.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIAQLLVETSPQKIIDLFSQYAQSDVY"
     gene            6797..7111
                     /locus_tag="SI621_001038"
     CDS             6797..7111
                     /locus_tag="SI621_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEI4063779.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAAEISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            7136..8227
                     /locus_tag="SI621_001039"
     CDS             7136..8227
                     /locus_tag="SI621_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEI4063780.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            8239..9090
                     /locus_tag="SI621_001040"
     CDS             8239..9090
                     /locus_tag="SI621_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEI4063781.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            9171..10031
                     /locus_tag="SI621_001041"
     CDS             9171..10031
                     /locus_tag="SI621_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEI4063782.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            10079..11011
                     /locus_tag="SI621_001042"
     CDS             10079..11011
                     /locus_tag="SI621_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEI4063783.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGVALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10992..11672
                     /locus_tag="SI621_001043"
     CDS             10992..11672
                     /locus_tag="SI621_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEI4063784.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGAAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRAVKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNHFLEPGGRFPAIEEDEPVKWLL
                     CSEYAVLR"
     gene            complement(11715..13424)
                     /locus_tag="SI621_001044"
     CDS             complement(11715..13424)
                     /locus_tag="SI621_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEI4063785.1"
                     /translation="MKFIGKLLLYIFIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAVNATKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(13545..14936)
                     /gene="xanP"
                     /locus_tag="SI621_001045"
     CDS             complement(13545..14936)
                     /gene="xanP"
                     /locus_tag="SI621_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HEI4063786.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            15216..16421
                     /gene="gltS"
                     /locus_tag="SI621_001046"
     CDS             15216..16421
                     /gene="gltS"
                     /locus_tag="SI621_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEI4063787.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(16455..18536)
                     /gene="recG"
                     /locus_tag="SI621_001047"
     CDS             complement(16455..18536)
                     /gene="recG"
                     /locus_tag="SI621_001047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEI4063788.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18542..19231)
                     /gene="trmH"
                     /locus_tag="SI621_001048"
     CDS             complement(18542..19231)
                     /gene="trmH"
                     /locus_tag="SI621_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HEI4063789.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(19238..21346)
                     /gene="spoT"
                     /locus_tag="SI621_001049"
     CDS             complement(19238..21346)
                     /gene="spoT"
                     /locus_tag="SI621_001049"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEI4063790.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(21365..21640)
                     /gene="rpoZ"
                     /locus_tag="SI621_001050"
     CDS             complement(21365..21640)
                     /gene="rpoZ"
                     /locus_tag="SI621_001050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEI4063791.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21695..22318)
                     /gene="gmk"
                     /locus_tag="SI621_001051"
     CDS             complement(21695..22318)
                     /gene="gmk"
                     /locus_tag="SI621_001051"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEI4063792.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            22576..24258
                     /gene="ligB"
                     /locus_tag="SI621_001052"
     CDS             22576..24258
                     /gene="ligB"
                     /locus_tag="SI621_001052"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HEI4063793.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGNETRDVMMPPLNGAVIHPVAHTGVRK
                     MADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTASGPLANSTLQGEIFLKREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELTTAGFTLTQTYTRAVKNADEVARVRNAWWKAKLPFVT
                     DGVVVRAAKEPESRYWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRVALNASDERS
                     WSQLLLSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(24255..24872)
                     /locus_tag="SI621_001053"
     CDS             complement(24255..24872)
                     /locus_tag="SI621_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEI4063794.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(25163..25987)
                     /gene="dinD"
                     /locus_tag="SI621_001054"
     CDS             complement(25163..25987)
                     /gene="dinD"
                     /locus_tag="SI621_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEI4063795.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENSVKITEKK"
     gene            complement(26208..27071)
                     /locus_tag="SI621_001055"
     CDS             complement(26208..27071)
                     /locus_tag="SI621_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEI4063796.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27198..27914
                     /gene="rph"
                     /locus_tag="SI621_001056"
     CDS             27198..27914
                     /gene="rph"
                     /locus_tag="SI621_001056"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEI4063797.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            27980..28621
                     /gene="pyrE"
                     /locus_tag="SI621_001057"
     CDS             27980..28621
                     /gene="pyrE"
                     /locus_tag="SI621_001057"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEI4063798.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(28658..29254)
                     /gene="slmA"
                     /locus_tag="SI621_001058"
     CDS             complement(28658..29254)
                     /gene="slmA"
                     /locus_tag="SI621_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEI4063799.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(29361..29819)
                     /gene="dut"
                     /locus_tag="SI621_001059"
     CDS             complement(29361..29819)
                     /gene="dut"
                     /locus_tag="SI621_001059"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEI4063800.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(29797..31017)
                     /gene="coaBC"
                     /locus_tag="SI621_001060"
     CDS             complement(29797..31017)
                     /gene="coaBC"
                     /locus_tag="SI621_001060"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEI4063801.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31189..31857
                     /gene="radC"
                     /locus_tag="SI621_001061"
     CDS             31189..31857
                     /gene="radC"
                     /locus_tag="SI621_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEI4063802.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            32074..32310
                     /gene="rpmB"
                     /locus_tag="SI621_001062"
     CDS             32074..32310
                     /gene="rpmB"
                     /locus_tag="SI621_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEI4063803.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            32331..32498
                     /gene="rpmG"
                     /locus_tag="SI621_001063"
     CDS             32331..32498
                     /gene="rpmG"
                     /locus_tag="SI621_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEI4063804.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            32596..33405
                     /gene="mutM"
                     /locus_tag="SI621_001064"
     CDS             32596..33405
                     /gene="mutM"
                     /locus_tag="SI621_001064"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEI4063805.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(33444..33923)
                     /gene="coaD"
                     /locus_tag="SI621_001065"
     CDS             complement(33444..33923)
                     /gene="coaD"
                     /locus_tag="SI621_001065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEI4063806.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(33931..35208)
                     /gene="waaA"
                     /locus_tag="SI621_001066"
     CDS             complement(33931..35208)
                     /gene="waaA"
                     /locus_tag="SI621_001066"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEI4063807.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            35621..36679
                     /gene="rfaQ"
                     /locus_tag="SI621_001067"
     CDS             35621..36679
                     /gene="rfaQ"
                     /locus_tag="SI621_001067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HEI4063808.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLVCVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            36676..37800
                     /locus_tag="SI621_001068"
     CDS             36676..37800
                     /locus_tag="SI621_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEI4063809.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEVE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            37793..38590
                     /gene="rfaP"
                     /locus_tag="SI621_001069"
     CDS             37793..38590
                     /gene="rfaP"
                     /locus_tag="SI621_001069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HEI4063810.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            38606..>39526
                     /gene="waaO"
                     /locus_tag="SI621_001070"
                     /gene_synonym="rfaI"
     CDS             38606..>39526
                     /gene="waaO"
                     /locus_tag="SI621_001070"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HEI4063811.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVSYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDAIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNY"
BASE COUNT         9471 a         9598 c        10522 g         9935 t
ORIGIN      
        1 acaggtcagt ttttttcgcc agctgttaaa ggaatagaat cgtcgtataa aaaaggtgtt
       61 ctggggcgta tgaaacaaca tcttcacctt aatggcgata cagttggctg agagccagca
      121 gggggtaaat cacctgcaaa taaacacggc acatgctggc gcgcatatcg cggatgcgtt
      181 gaggacagaa gaaccgacag gcggattctt cagccgctca tcgtccatca caaaaccttt
      241 gaccaggtat tcctgaagcc gattgaatat tgagcagtag aaaatatctg gattgacagt
      301 attacaaggg attggagaac gtcagaagag gtattttggc ggaagatcac aggagtcgaa
      361 cctgcccggg accgctggcg gccccaactg gatttgaagt ccagccgcct caccggagac
      421 gacgatcttc cgcgcctcga ttgctacatg gaggcggggc gcattatagc tacttccttg
      481 agtttctaca tcccccagat cagttttttc cgcctaatac acgcagcgta ttatcacgga
      541 agcattataa ttcatataaa acccatattt tacaatgata tatgagcgga attaacgcta
      601 ccacgatcac ataaaacaag aatcataaat taataaccag atatcggaat attcgctctc
      661 acagggatgg tttacaaaat gcgttatcgg tctcagcacc cctatagcat caaggaaaag
      721 cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg gttatggcat gggagacgcc
      781 gccagccaca ttattttcga taacgtaatg ttatatatga tgttctttta taccgatatt
      841 tttggcattc ctgccggatt tgtcggaact atgtttttgg tcgctcgtgc actggatgcg
      901 atttccgatc cttgcatggg gttgttggcc gatcgaacgc gctctcgctg gggtaaattt
      961 cgtccgtggg tactgtttgg cgcactgcca tttgggatcg tctgtgtact ggcctatagc
     1021 acgccagatc tcagtatgaa cggaaaaatg atttatgcag caattactta caccctactt
     1081 accttacttt ataccatcgt taatatccct tactgcgcat tgggtggtgt aatcaccaat
     1141 gacccgactc agcgtatctc gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc
     1201 atgctctcta ctgtgctgat gatgccgctg gttaatttaa ttggtggtga taataaacca
     1261 ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg cattcatgat gctggcattt
     1321 tgtttcttta ccactaaaga acgcgttgaa gcaccaccta caacgacgtc tatgcgggaa
     1381 gatttacgtg atatctggca aaacgatcag tggcggattg tcggtttact gaccattttc
     1441 aatattctgg cggtgtgcgt acgcggcggg gcgatgatgt attacgtcac atggattttg
     1501 ggtacgccgg aagtgtttgt cgcttttctt accacttatt gcgtgggtaa cctgattggt
     1561 tccgcactgg cgaaaccgct gaccgactgg aaatgtaaag tcactatctt ctggtggacg
     1621 aacgccctgc tggcagtgat tagcctcgcg atgttctttg ttcctatgca ggccagcatc
     1681 actatgtttg tcttcatctt cgtgattggt gtgttgcatc aactggtgac acctatccag
     1741 tgggtaatga tgtccgatac cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc
     1801 gggatcagtt ttgctggcac gctgtttgtg ctcaagctgg ggttggcctt cggcggcgct
     1861 cttatcggct ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc
     1921 gccacgatta gcatcattat cgcgctattc acgattgttc cggcgatctg ttatttgctg
     1981 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
     2041 gaacagctgg ctcagggcaa acgccgttgc cagcaacaat tcacctctca aaatgtgcag
     2101 aactaaggaa cggcaatgaa aattagcgac ggaaactggt tgattcaacc tggcctcaat
     2161 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg gtaatgaaat ggtggtctat
     2221 gctgcccctc gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg
     2281 cgctttttct ccccacagga aggtattgtc ggtgtacgga ttgagcattt tcagggggcg
     2341 ctgaataacg gtcctcatta cccgctcaat attttgcagg acgtgaaggt cacaatcgaa
     2401 aacacagaac gttacgctga gtttaaaagt ggcaacttaa gtgcgcgtgt cagcaaaggt
     2461 gagttctggt cactggattt tctgcgtaac ggcgaacgta ttaccggtag tcaggtgaaa
     2521 aataatggct acgtgcagga cacgaataat caacgaaatt atatgtttga gcggctggat
     2581 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgcgct ggtgcgcaat
     2641 ggccagacgg tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa
     2701 aatatcccgt tctatatgac taaccgtggc tatggggtac tggtcaatca tcctcaatgc
     2761 gtctcttttg aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa
     2821 tatctcgaat actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc
     2881 cgttttactg gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact
     2941 tcatttacca ccaactacga cgaagcgacg gtaaacagtt ttatcgatgg tatggcggaa
     3001 cgcaatctgc cactgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg
     3061 tgcgattttg agtgggaccc gctgactttc ccggacccgg aagggatgat ccgccgcctg
     3121 aaagcgaaag ggctaaaaat ctgcgtctgg attaacccct atatcgggca aaaatcccct
     3181 gtctttaaag agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgttatgg
     3241 cagtgggata aatggcagcc aggtctggcg atttatgact ttaccaatcc ggatgcctgc
     3301 aaatggtacg ccgacaaact gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc
     3361 gactttggcg aacgtattcc aaccgatgtt cagtggtttg acggttccga tccgcagaaa
     3421 atgcataacc attatgcgta catctacaac gaactggtgt ggaaagtgct caaggaaacc
     3481 gttggcgagg aagaagccgt cttgtttgct cgctcggcct ccgttggtgc gcagaaattc
     3541 ccggtacact ggggtggtga ctgttacgct aactacgaat caatggcgga aagcctgcgc
     3601 ggtggtttgt ctattggcct ttcaggtttt ggtttctgga gccacgatat cggcggcttt
     3661 gagaataccg cgccggcgca cgtttacaaa cgctggtgcg cgtttggttt gctctccagc
     3721 catagccgtt tacacggcag caaatcttat cgtgtgccgt gggcctacga tgatgagtcc
     3781 tgtgatgtgg tgcgcttctt cacgcaactg aaatgccgca tgatgccgta tctgtatcgt
     3841 gaagctgctc gtgcgaacgc gcggggtacg ccgatgatgc gggccatgat gatggagttt
     3901 ccggacgatc cggcttgtga ttaccttgac cgtcaataca tgttaggcga caacgtgatg
     3961 gttgcgcctg tgttcactga atcgggcgat gtgcagttct acttgcctga aggtcgctgg
     4021 acacacctgt ggcacaacga tgaactcgat ggtagtcgct ggcataaaca gcagcacagc
     4081 ttcctgagtc tgcccgttta tgtgcgtgat aacaccctac tggcgctggg caacaacgag
     4141 caacgtcccg attacgcgtg gcacgaaggc acggcattcc agctctttaa tctacaagac
     4201 gggcatgaag ccatctgtga agtgcccgct gctgacggtt ccgttctttt caccctgaaa
     4261 gcggcgcgta ctggcaacac gattactgtg actggtgcgg gcgaggcgaa gaactggaca
     4321 ctgtgcttgc gcaatattgt gaaagtaaat ggtctgcaag gcggttcgca ggctgaaagt
     4381 gagcagggac tggtggtgac tcctcaaggg aatgcgctga caattacgtt gtaatctatt
     4441 gaagaatgcc ggatacgaat ggttttgtgt cgtatccggt aaataatctc acatctggta
     4501 ataaatattt cttccatcgt atttgcgagc atgatcacga ttattcatca cgaaaatgtg
     4561 atctgtgccg aaaaatatct attaaccgcc ccgttttgtt ttatacatgt ccggactacg
     4621 gacagatttt agtattgttg tgaaattatt ctgctttata tttaccctca gagatggcaa
     4681 ctgaagctct tccacatggg gcttcttaat ctgtgataag ggtaaaaaat gcaaatgatt
     4741 acctcgcgac aaaacagatt attacgattc cttttaccac gaagggaata tacgactatt
     4801 gttacgattg ccggctattt aaatgtttcg gaaaaaacca ttcaacgtga tttacgttta
     4861 cttgagcaat ggctggaaca atggcgaata accgttgaga agcgtgctgg cgcgggtgtg
     4921 atgttagact cgaagaatat tactgatttg ctgcatcttg atcatttgtt gggagcagaa
     4981 tgtgaagaga ttgatggtgt aatgaataat gccaggcgcg ttaaaatagc gtcgcagtta
     5041 ttaagtgaaa caccgaatga aacgtcgatc agtaaattgt cagagcgcta ttttatcagt
     5101 ggagcctcta ttgttaatga cctgagagtg attgagtcct ggcttgcgcc gttggggtta
     5161 tcattgatcc gcagcccaag tggtacgcat attgaaggta gtgaagggca agtccgacag
     5221 gcaatggcat tactgattaa cggcattatt aaccataatg agccgcaagg tgtcgtgtat
     5281 tcacgtctgg atcccggaag ttataaagca ttagtccatt attttggaga agaagaggtg
     5341 ttatttgtcc agtcattgtt actggatatg gaaaatgaat taagttggtc tttgggagag
     5401 ccttattacg ttaacatttt tactcacatc cttattatga tgtatcgcaa cacgcacggg
     5461 aatgcgttat ccagagaaga agatcaaacc aggcaatatg atgaaaatat ctttaatgtt
     5521 gccagtcaga tgattcataa gatagaacaa cgaattgcac atacattgcc cgatgatgaa
     5581 gtctggttta tttatcaata tatcatttca tcaggtgtgg cgattgatgg acaaaaagat
     5641 gtgagcatta tttcacatat gcaggccagc aatgaagcgc gtctgattac ctggcgttta
     5701 attacggtat tcagtgacat cgtggactgc gattttagtg aagacagcgc attatatgat
     5761 ggcttgttgg ttcatattaa gccgctgatt aaccgactaa attatcgcat tcatatccgt
     5821 aatccattgc tggaagatat taaagcagaa ttagcggatg tctggcggtt gacgcaatat
     5881 gtggtgaatc aggtatttaa aacctggggt gagaatgcag tgagcgagga tgaagtaggt
     5941 tacctgaccg ttcattttca ggctgcgatg gagcggcaaa ttgcccgtaa acgtgtatta
     6001 ctggtctgtt caaccggaat cggaacttcg catctactga aaagccgtat tctgcgagca
     6061 tttcctgaat ggacgattgt tgatgttatt tcagcagcga atttatcaca ggttttgcct
     6121 gacaatatcg aactgattat ttcgaccatt aatttgccta cagtcactat gccggtcgct
     6181 tatgttaccg ctttttttaa tgatgccgat attaagcggg tcactgaaat ggtgattacg
     6241 gaaaaattac atcatgcgac atctcgggtc gttgaaattt aatgtcagaa ttttatgagg
     6301 tatgacgaat ggacataaca aaaatcctga atacgaatcg cgttatttta gatatgcaag
     6361 cgacgaataa agcggaggcg attgaagaat taactaatct tctcaaaaaa gatggtgcga
     6421 tcgattgtcg ggaaaccttt attaaagatg tctggcagcg cgaagcggaa ggctcgactg
     6481 gttttgaaaa tcatattgcg atcccgcatg gtaaatcttc tgcggtaatt aataccacgc
     6541 tggctatcgg gcgtacccgg caggatattc cctgggaaac actggatggc agtagcgtac
     6601 gctgtattat tttatttgcg gtacgtcttg aagatcaaaa taccactcat atccgtttac
     6661 tttcccaggt cgcgggcgcg cttgcagatg atgacatcat agcgcaattg ctggttgaga
     6721 cgagcccgca aaaaatcatt gatctcttta gtcagtatgc acaatccgat gtctactaaa
     6781 taacagggag tatatcatga atattgtctg tgttgccgcc tgcacggcgg gtattgctca
     6841 cacctatatt gctcgtgaaa agttaattaa aggggcaaaa gcactgggcc acaccattaa
     6901 agttgaaacc cagggaacca ttggtacaga aaacgaatta caggcggcag agatctctgc
     6961 tgctgacgtc gtgattcttg ctgtggatgt gaaaataaaa ggagaagaac gttttaccaa
     7021 taaacgcatt gtgcgagtaa agacagagat cgtcattaaa tcgcccgttc aatttcttga
     7081 aaaggtcgaa aaatcactgg gaaataaatg atccttatac tggagggatc ttaatatgaa
     7141 agaaaataaa attccggttt cacaggaaat taaaaaacat ttattaaccg gcatctcgtg
     7201 gatgataccc cttattgtgg ccgccgggat atgtattgcg ttggggcaag ttcttggcgg
     7261 tacagatgtt ggtgaaaaaa caggaacaat cccgtggatg cttaatcaga ttggcggctg
     7321 gggaatgggg ctgattgtgc cgctgattag cgcggcaatt gcctattcta ttgccgatcg
     7381 tcccggattt gccccgggcc tgattgtcgg ctttctctgt gggcaaatcc ataccggatt
     7441 tattggcggt atgttgggtg gtttcctggt gggctacacc attttgctac ttaaacgcta
     7501 tattcgcctg ccgcagtcga tgcaaggact catgccaatc atggtgttac cggtattgag
     7561 caccattatt ggtggtctgt taatgatgac gctgattggt aaacccatcg cctggttaca
     7621 agaagcactg attcatttgc tcgagtcgat gcagggcgga tcgcgcttcc tgatgggggc
     7681 catacttggc gcgatggcaa ccttcgattt cggtggtccg gtcaacaaaa ccatgtcgct
     7741 gttttccgat ggcttgctgg tcagtggcgt ctacggacca gaagccgtga aatttgtcgg
     7801 ctcaatcatc cctccgtttg gcatcacact ttcgttctta ctgacacgcc ataaatacac
     7861 ccgtgcggag cgcgaggcgc tgaaagccgc gtttccgatg gggatctgca tgatcactga
     7921 aggagtcatt ccgatcgccg cgcgtgacct gttgcgagta gtgggatctt gtgtggtggc
     7981 atcggcggtc gcgggcggct taatcatggt gtggggcgtg gagagtccgg ttccacatgg
     8041 cggcatgttt gttgtgcctc tgttcaccca tcctctgctg ttttgcctgg cgctgggtat
     8101 aggcacggtg atttgtggcg tgatgctttc actctggaag aaaccggtaa cggaacgcga
     8161 cgaagagttt gacgaactga gcgatcaaaa gctgaaagat gaagagatca ctttcaccct
     8221 cgaataaccg gaggccctat gtttgccgat atgaaaagta tggtgatgaa agcctggcat
     8281 gaacattacg ccttgctggc gatcaactgt atgaatctgg aaagtgctca cgcagctatc
     8341 cgcgtggcgg agaagaatcg cgctccgatc attctcaatc tctatcaggg gcatctggcg
     8401 cattttcctg cacccgtcgc ggcggcagtg gttaaaacgc tcgcggaatc tgcctcagta
     8461 ccggtcgctc tggcgcttga tcatggcaaa aatccggact gtattcgtca ggcgtttcgt
     8521 gctggtttca gcggattaat gattgatgct tctgcttttc ccctggagga aaacgtgcgc
     8581 cagaccaggg cggtggtgga actcgccgcc agcgcgcagc tctgcgtgga aggcgaactg
     8641 ggccatctgg cagatgcgcc gcgttacgac caggccgcca atgcagacct gatgacgcaa
     8701 cccactgatg tggaaccgtt tatcgcgcag acaggcattg atctactggc cgtctcggta
     8761 ggcacggcgc acggcatgta cgcgcctggt gttgtacctg ctattgattt tcagcgtctg
     8821 gcggagattt tccactgctc gagcgtgcct ctggcattac atggcggtag cggtacgccg
     8881 ttcgatcagc tccagctctg tacgacgctg ggggtagcaa aaatcaacgt gggggcggcc
     8941 atttttgaac gggggaaatc cgcgctcctg cataccctct gtcacgatat atccattgaa
     9001 ctggtggacg cgctgaaagc tatggaactg gccttcgaag aagcgattac accgtattta
     9061 caggcgagcg gctctatagg caaagcctga ttcattttct gtcatgacct ttatgccagt
     9121 gcaataacgc accggcaggg cggactacac gctgaaactg gagatttaac atgttagttt
     9181 cgatgaaaga attactgcaa cctacccgcc agcacggctt tgccattggc gcatttaatg
     9241 tggcggataa ctgtttcctt cgcgcagtcg tggatgaagc agaagcgaca aatactccga
     9301 cgattattgc catccatccc agcgaacatg attttgtggg cgatgccttt ttcgctcacg
     9361 ttcgcgaaat tacccagcgt agtccggtcc cgtttgtact gcacctcgat catggtgcct
     9421 ctgtagagca tgttttgcgt gcgattcgct gcggatttac ctcagtaatg atcgacggtt
     9481 cgctgctgcc gtatgaagaa aacgtggcgc tgaccgcaga agtggttcgc ctcgctcacg
     9541 cggttggagt gtcggttgag ggtgaactgg gaaccatcgg acaaacagga acgtcggtag
     9601 aaggtggtgt ttcacaagtg acctacacag atccggcgca ggccgccgat tttattgctc
     9661 gtacaggtgc cgacacactg gctgtggcga ttggtacagc gcatggcatc tatccaaaag
     9721 ggatgcagcc gaagctgcaa atggacattt tgcgcgacat tgccgggcgc attgatattc
     9781 cattggtgtt gcacggtggt tcggcaaatc cggacgcgga gattgctgaa tcagttacgc
     9841 tcggcgttgg caaaatcaat atctccagcg atatgaaata cgcctatttc cagaaagtgc
     9901 gtgaaattct cgccaaagag agctggtggg atccgaacgt catctatccg gatgcgatta
     9961 acgcggcgcg ggaagtcatt cgccacaaaa tgaaactgtt cggttcgctc ggtaaagcat
    10021 cactgtacta agccagaagg gcgggatgac ccgcctttta cgcaacaagg aacgagatat
    10081 gcactatctg gggttggata ttggtgggac caaaatcgcc gccgtcgtca tggatgcgca
    10141 tggctgggag attcgccgtt accgctgccc gacgcaaaag tcgacatatc aacaatttgt
    10201 ctcatgcgtt gtggcgctta tcgagcagat taggcgggac gttcaacgac cgatgctgac
    10261 aggggtcgcc ttacccggca gtatctcgcc actcactggc ctgattaaaa acgcgaatat
    10321 tcaggtgatt aacggtcatg cgttacaggc tgatttgcag caattgcttg ggcaaccggt
    10381 ggtgatagcc aatgatggta actgtttcgc gctatcagaa gcttgcgacg gtgccgggca
    10441 agattatgac gtagtatttg gtattacgct tggtacaggc tgcggcggtg gcattgccat
    10501 caagcaacga ccgtttatag gggcctgggg aaatgctgcc gaatgcggtc atatcacgtt
    10561 gccaggctat atggagcagg aagatggtcc atcagtcagt tgctattgcg gcaaacacaa
    10621 ctgcgtggag tcgtttgttt ccggcagcgg ttttagtgaa cgctatcaac agatgactgg
    10681 taacttgctc actcctgcgg cgattgtcac cctggcacaa cgtggtgatg cttgtgccat
    10741 gcagcaggtg gcacgttttc gccaacagct tgcccgcacg ctggcaacca tcgttaacgt
    10801 tgttgaccct ggcgtgattg tcatcggcgg cgggctttcg aatgtggaac tgcttatcac
    10861 cgatctgaac gcagaagtcg ctccactggt tttcaccgat caattcacca ctcccattgt
    10921 aaaagcacag cacggcgaca gtagcggaat gcgtggcgct gcctggcttg ctatgcgcaa
    10981 cggagaagcc aatgaaacgt tcacaaatta attatgttat tgataaagca catgccattg
    11041 cgcagacctt ccgcgtttgt ttgccagagt tcgcctattt tacggtggat gactggctgc
    11101 agcgggaacg ggataactgg caagaagtgg tcgacttaca gctcggttgg gatatcaccg
    11161 attttggtcg tggtgatttc aaccaaacgg gactaacact gctaacgatg cgcaacggcg
    11221 cgctgggggc ggcggcatat ccaaaaccgt atgctgagaa aatgttgcag atccaacaag
    11281 atcagcagac tccctggcat tttcacaccc acaaaatgga agatatcctc aatcggggcg
    11341 gtggagactt atgcatgcag ttggcctggg ccaacgaagc caatctctgt gatgatcagc
    11401 gggttattac cgtgagtgtt gatggtcagc ggcgtgccgt gaaacctggt gaaacgctgg
    11461 tgctaaagcc ggggcagggc atttgcctgc cgccgcgtct gtatcatcgc ttctgggcgg
    11521 aaaaagcgtt tgttctcggc tgggaaattt cgatggtaaa tgatgaccaa catgataacc
    11581 attttcttga gccgggagga cgttttccgg cgatagaaga agatgaaccg gtgaaatggt
    11641 tattgtgtag tgagtatgct gttttgcggt aatagcgggg acgctaacgt tgatatccgt
    11701 tagcgcccgc gtatttactg caccggctcc gtcgatgtaa cttcgccact ggaaacgacg
    11761 cctgcattca tggtttgagt cacttgctgc tttgtggcgt tcacggcatg caattgcccg
    11821 ctaaccgtag gtttcagcgg gatattggcc tgaatatcgc cgctggcggt aagctgaata
    11881 ttgccgtcgc cagtcaacgg taattcaggc catccccact gttgcaaaat attcaccggc
    11941 acaccgcgtc cattcaggct gatatgggtc tgacgttgtg gcgtttgtga aacactggcg
    12001 gtggcttcca gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg
    12061 ctgttggcgg tcagcgccag cgatgggcga cgaacatcaa cacgattaaa tgtcgcggcg
    12121 gcggcattca gattcgccga accactccag acgccccatt tatgatcggt aaccagcgtc
    12181 aggttggcac cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca
    12241 atgatcagat tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt
    12301 gttgtttcca tccacaactg ttgccagttt tttggcaagg tatattccag tccggcaatt
    12361 gccgcatcat cgaggatcag cgttttcccg tcgcgttgcc agtttcctga cgttctgacc
    12421 ataccccctt cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc
    12481 gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct ggcgttcatc
    12541 gacagtttgc cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg
    12601 agatcgaggt cggtcaccgc ccagtccgga ccttgcaaac gagcatcgat cacttccagg
    12661 cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt
    12721 tcgctttgta gacggatatc cgccatgcgc agattttcca cttgccagct gccgtcggcg
    12781 ttacgctgcg cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc
    12841 gtaacgcgat cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg
    12901 agcgaaagcg atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct
    12961 tctggtgacc acggaaccac gccgccattt acccgctgcg cgctcagttt ccattcgcta
    13021 ttcgggctat taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc
    13081 gcggtctggt cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt
    13141 ggttcggtca gttgccgact gcttagcgca atgtcgacac tttttgccac cagggtcgcg
    13201 ggctggccat cacggccgaa cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa
    13261 cggtgatcca tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg
    13321 atatgttctg ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac
    13381 agagcgatga aaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg
    13441 aaatgcacat aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa
    13501 agaggtgaga ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt
    13561 gggaaaatca gattcagcac gatggcagta ataccgcccg cggcgatccc ggaggagagc
    13621 aggtttttca gccactcagg ggcaaactgc aaaatcagcg gctgctgaga gacgcccaga
    13681 ccaaccgcca gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ctcacgagac
    13741 acaatgcgca caccggaggc ggcgatggtg ccaaacatta caagtgttgc gccgcccaga
    13801 accggttctg gaatatgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc
    13861 aacatcagcg cgacgacaaa accgacatag cggctggcaa ccccggtcaa ctggatcact
    13921 ccgttgttct gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag
    13981 ttcaggccgt ttgccagcac gccgcctttc aggcgtttca tgtacagtgg accggacact
    14041 ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag tgaagtgatc
    14101 ataaagacca gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc
    14161 ggcgttggca ccataatcag ttcttgcgtc atcggttcgt tgctttctgg caacatgccc
    14221 ataaaccacg ccagcgcata tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag
    14281 taagggttac gttgacggtt aagcaggata attaaggcta agaccacgcc tgccagcagc
    14341 agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggaa
    14401 gtcagcccaa cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc
    14461 ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtgcaactt
    14521 gccagcatca acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtc
    14581 ttcagcgctg tgccgcccat aatcagcggg gcaacaaagt tgaagctggt gccctgaata
    14641 gacaacaacc cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg
    14701 gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc
    14761 tggcagatta atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag
    14821 gcggcaaaca gagtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg
    14881 ttttgagtct gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa
    14941 aacaatcccg tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt
    15001 gccacatcct catctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt
    15061 atttttctgc ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa
    15121 agcataactc gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga
    15181 gtatgaaaga gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa
    15241 cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct
    15301 ttttgaagaa atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc
    15361 tactagtact gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc
    15421 cgttaatgct ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg
    15481 ccggtgggcg tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa
    15541 atgccattgg cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg
    15601 ccggttctat tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca
    15661 ttgaacgtta tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc
    15721 tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca
    15781 cgccgaacgg tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg
    15841 gacgcatgat cacctcgttg gtgctgattg aaactatcgc gctgattgct atctgcctga
    15901 cggtggggaa aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg
    15961 tctgtgtcct gtttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc
    16021 gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga
    16081 tggcgctgat ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga
    16141 ttctggtggt acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga
    16201 tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggtca ttgtggtttt ggcctcggtg
    16261 caacgccaac ggcaatcgcc aacatgcagg cgatcactga acgttttggc ccgtcgcaca
    16321 tggcgttcct ggtagtgccg atggtcggtg cgttctttat tgatatcgtt aacgcgctgg
    16381 tgattaagct gtatttaatg ttgccgattt ttgcgggcta accgatgaag cggcggtctt
    16441 ctcaccgccg ccttttacgc attcgagtaa cgttccgtct ccggcatcca gcgttctatc
    16501 agggcttttg cttgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc
    16561 gggatcatcg cctgatcgcg cagtaaatcc gccactttaa actcagcatt acccgtctga
    16621 cgcgtaccta acagttcacc cggtccgcga atctctaagt ctttctgtgc aatcacaaaa
    16681 ccgtcgttgc tgtcgcgcag cacttgcagc cgaatttgcg cggttttgga gagcggcgtt
    16741 ttgtagagca gcacgcagtg agaagccacc gcaccacgac ctacacgccc gcgaagctgg
    16801 tgcaactgcg ccagacccag acgctccggg ttttcgataa tcatcaggct ggcgttaggc
    16861 acatcaacgc cgacttcaat aacggtagtg gcaaccagca ggtgtaactc gccttgttta
    16921 aatgatgcca tgacggcctg cttctcggct ggtttcatcc gtccgtgtac cagaccaaca
    16981 ttcaactctg gtagcgccag tttcaactct tcccaggtag cttccgccgc ctgcgcttcc
    17041 agtaactctg actcttcaat gagtgtgcac acccagtacg cctgacgccc ttcagtcata
    17101 caggcgtggc gcacgcggtc gataatgtcg ttacgacgcg tatccgggat tgccactgtc
    17161 gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag atcggcatac
    17221 gcggtcatcg ccagcgtgcg ggggatcggc gtcgcggtca tgatcaactg atgcggatgg
    17281 aagccctgct gctggccttt ctcccataac gccagacgct gatgcacgcc aaaacgatgc
    17341 tgttcgtcga taatcaccag cgccagaccg ttaaactgca cctgttcctg gaagatggcg
    17401 tgtgtaccga caatcatctg cacctgaccg ctggcgatgg cttcctgctg tgccagtcgc
    17461 gctttacctt tctgcttacc ggcgagccag cccacttcga taccgagcgg tgcaaaccag
    17521 ttgcggaagt tattcgcgtg ctgctcggcg agtaattcgg ttggtgccat caatgccacc
    17581 tgtttgccgt gggcaatcgc acgcaacgcg gcgagggcgg cgaccagcgt tttaccggaa
    17641 cctacatcgc cctgcaccag gcgcatcatc ggcgcatcca gcgccatatc gcgctcgatc
    17701 tccgccacca cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggctgc gaggagttta
    17761 tttttcagcg cgtcattggc gctaagcggc tgggcatgga aacgttgtgc accagcgcgt
    17821 aacgccaaca tgctgaggtt gtgcgcaagc agttcttcca gaatcagacg gcgttgtgct
    17881 ggatgctgcc cggtttcgag atcgctaagc tgtagcgtcg gcggcgggcg gtgcaaagtg
    17941 cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag gagttcttca
    18001 atggcgcagg tgtcgagcag atccagcgcc tggtcggtta atttacgcag cgtggcctgc
    18061 tttacgcctt cggttgttgg gtaaaccggc gtaagcgttt cctgcaactc tggcgtactg
    18121 agatcgccct gcacgcggta ttccgggtgg atcatctccg cgccatattt accgcgcttt
    18181 gcttctccat aggccagcac gcggcggccc gtcgccaggc tatttttcat tgccgcgttg
    18241 aagttgaaaa agcgcatggt gagaatgccg gaaccgtcgc tgatctggca ggtcatcatc
    18301 cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt ggcataaacg
    18361 cccggtagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg caggggaagg
    18421 tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagcttgtt actaagcgct
    18481 gcaccaacgc ccgttaggga actgagcggg acagcatcta acaggcgacc tttcatggta
    18541 cttaccctgc agcttgcata gtggcccacc agtcggcatc ggcttcaatt tcgccctgct
    18601 gattgacgtg ggggtaaggc aggcctttgc gttttgcgac tttcgccagc accggatagc
    18661 cgccttcaaa cagcaggcgt tgttgctctg cttccggcaa catgctgttt tcacgcaggt
    18721 acatgcccgc attttgccgc tggcgctggg cttcgtaaag aatgagggct gaggcaacgg
    18781 aaacattcag cgattgcacc atgccaatca tcggaatgat gatgtcccga tccgccaggg
    18841 ccaacgcttc ctgcgtgatg cctgttttct cctgtcccat caaaatacag gtcgggcgag
    18901 tgtaatcaat ttcgcggaaa tcgacagcgt tatcagaaag atgggttgcc agaatctgca
    18961 tgccctggcc tttcagatga gcgacggcat cgccaatggt gcggtgcgtt ttcacctgta
    19021 cccagctgtt actacccgca gctgccgaag ccatggtgcg catccggcta ccaggccaga
    19081 cagcatgaac ttcatgtaca ccaacggcat ctgcggtacg aataatcgca gaaacgttat
    19141 gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg gcgagcattt
    19201 cgcagatacg tgcataacgt gttgggttca taaaacatta atttcggttt cgggtgactt
    19261 taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc acacggtcac
    19321 gggcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac
    19381 tttgaatatt cgaggtggtg gtgttaattg ccgctgtcag gtttgccagc gcgccctgat
    19441 gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct ttatcccatt
    19501 ccacagccat aaacttctct ggctctttct ggtagccacg gatgttacgg caggattcat
    19561 ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg ccaggaatag
    19621 ggcggcagca tttcgcaaag gtgatcagca cgccatcggc acctttaatg ggcagatgtc
    19681 cgtgactttg ggttgccggt ggaatggagg cgtccccatg ttgcagattt ttcgcgacca
    19741 ccacgctcat tgcgttacca aggccgattt cagccagcag atcgtcaagc gttgccagct
    19801 tcatgcggtc cagctcgcgc tgaatatttt cctgcgggat ttcattgagc ttgcggctgc
    19861 cacccaacgc atggttgagc agacgacggc ccaggcttac agaatcatca cgcttgaggt
    19921 ttttcagcaa ttgacgaatt ttggcgcgcg ctttcgagct aacgacaaag ttcagccacg
    19981 cggcattcgg gcgagcgccc ggtgcggtaa taatttcaac ggtttgaccg ctggtcagcg
    20041 gctgcgacag cgggtaaggc tggcggtcga cacgcgcgcc cacgcaggca tgaccgatat
    20101 cggtatgcac cgcataagcg aagtcgacgg gcgttgcacc ggcaggcagc tctacaatgc
    20161 gcccttccgg tgtgaaaacg taaatttcat ccgggaagag atcggattta acgctctcga
    20221 taaattcaaa cgagctaccg gcactctgtt gcagctccag caggctttgc atccagcgct
    20281 gggcgcggat ttgtgcggta gtactggttt cgccgtgctc tttataagcc cagtgcgcgg
    20341 caacacccat ctctgccatc tgatccatat cttcggtacg gatctggacc tcaaccggca
    20401 cgccgtgcgg gccaatcatc gaggtgtgca aagactgata gccgttcgct tttggaatgg
    20461 cgatgtagtc tttcacgcga cccggacgcg gcttgtacag gctatgcatt tgcccgagca
    20521 cgcgataaca ggtgtcggaa tcattgacga tcacgcggaa ggcgtagatg tccatgattg
    20581 agtgaaaacg ctgctctttg agcaccattt tgcagtagat cgaataaaga tgcttttcgc
    20641 gaccactgac gcggcacggt attcccgctt cctgcaaacg cccttcgatt tcagaaagaa
    20701 tcttctggat catctcttta cggttgccgc gcgcggcttt caccacttct ttgattacgc
    20761 gataacggtt gggatacagc gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt
    20821 ggatacctaa acggtgcgcc agcgggctgt aaatttcgag tgtttcacgg gcgatgcggc
    20881 gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt
    20941 tgatgagaat gacgcggata tcctgcacca tcgccataat catcttgcga aagttttcgg
    21001 cctgcgcctc tttcttatcg cggaacttga gcttatcaag tttcgacacc ccctctacca
    21061 gctcggcgac gcttttacca aaaagctgtt ccatatcctg ataggtggcg ggtgtatctt
    21121 caatcacgtc atgcagcagc gccgccatta gcgtttcata gtcgagtttc atctcggcca
    21181 gaatgcaggc aaccgctacc gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc
    21241 cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt tggtcttccg
    21301 gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc gacccgcttt
    21361 gtgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg gcttcctgct
    21421 cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga ccttcttcga
    21481 tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc ggatcctttc
    21541 cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca aaacggttac
    21601 caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag ctccacaggt
    21661 gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat ttgctgatta
    21721 aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga ataatggtct
    21781 tcaaatcggt caacgcggta tcgaagtcat cattcacaat caggtaatca tattcggcgt
    21841 aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct tcgctgtcct
    21901 gaccgcgacc gcgtagacgg cggtccagtt caattttgga cggcggtaaa ataaagatac
    21961 tccgcgcgtg cggcatcttc tggcgaattt gctgcgcacc ctgccagtcg atatcgagaa
    22021 aaacatcgac accggtcgcc aggacttgct caatggcctc acgcgaagtg ccatagtaat
    22081 taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct ttaaattcat
    22141 catgattaac aaagaaataa tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg
    22201 tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa gcctgaatca
    22261 ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct tgagccatga
    22321 gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta tacacggcgc
    22381 gggcatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc
    22441 tgcttccgca tttgcctgtc attcactgca ataaagacga ttctcaggaa gcgtcctcgc
    22501 agactccgtt tttgccactc ggctttgctt tcatcacgtt gtatatctgc ctgactatct
    22561 acagcggagg tgatgatgaa agtatggatg gcgatattaa taagtatctt gtgctggcaa
    22621 tcatcggcgt gggcagtctg tccggcctgg tcgccagcca gagcacagga agaaatttcc
    22681 cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaagaagg gaaaagtgag
    22741 gtggaagacg gtatttacga tcagttaagc gcccgtctta cgcaatggca acgctgtttt
    22801 gggaacgaga cccgcgatgt gatgatgccg cctttaaacg gtgcggttat acatccggtt
    22861 gcccatacgg gtgtacgcaa aatggcggat aaaatcgcat taagtctgtg gatgcgcgaa
    22921 cgtagcgatc tttgggtgca gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg
    22981 gacgggaaac tgaacaaagc aatcagtcgc ggcaatggcc tgaaaggcga ggactggacg
    23041 cagaaagttc gcttaatttc cgctgtgccg caaaccgcta gcgggccttt agccaacagt
    23101 acgcttcagg gggaaatatt tctcaaacgc gaggggcata tccaacaaca aatgggggga
    23161 ataaatgccc gcgcaaaagt tgctggtctg atgatgcgcc aggacgatag cgacacgctg
    23221 aattctctgg gcgtttttgt ctgggcatgg ccggatggac cgcagttaat gaccgatcgt
    23281 ttaaaagagc tgactaccgc tggttttact ctaacgcaga cgtatacccg tgcggtgaaa
    23341 aatgctgatg aagttgcgcg cgtacgcaat gcgtggtgga aagcgaaatt acccttcgtc
    23401 accgatggcg tggttgtgcg agcggcgaaa gaacccgaat cccgctattg gctaccaggc
    23461 caggcagaat ggttggtggc ctggaaatat caaccggtag ctcaggttgc cgaagtgaag
    23521 gcgattcagt ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc actcgcacct
    23581 gtcatgcttg atgataaaaa agtccagcgg gtgaatattg gttccgtcag gcgctggcag
    23641 gagtgggata ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca gggtattcct
    23701 cgcattgatg atgtggtgtg gcgcggtgca gaacgcacaa aaccgacacc gccagaaaac
    23761 cgctttaact cgttgacctg ctactttgct tctgatgttt gtcaggaaca gttcatttca
    23821 cgcttagtct ggctggggtc aaaacaggtt cttgggctgg atggcattgg tgaggccggc
    23881 tggcgcgcgc tgcatcagac tcatcgcttt gagcatatct tttcctggct tttattaacg
    23941 ccagagcaat tacagaacac gccggggatc gcgaaaagta aaagtgcgca gttatggcat
    24001 cagtttaatc tggctcgtaa gcagcctttt actcgctggg tgatggcaat gggaataccg
    24061 ctaacccggg tggcgcttaa tgccagtgat gaacggtcct ggtcgcaact tttattgagc
    24121 acggagcagt tctggcagca gctgccgggg actggatcgg ggcgcgccag acaggttatt
    24181 gaatggaagg aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc ccagcagatc
    24241 acaggttttg aaccttagtg tccttcgtgg ctgtagtaaa agaccggtaa tcccagctta
    24301 aggcgtagcg ccagtaaacg ggcaaaaaag ccgaatacca gggtggagat aattaccaca
    24361 tcatgattag aaacatagtg ttgcagcgcg atgtacagca cggcggaggc aaatgagaca
    24421 ccggcataca attctttctg gaataccagc gggatgcgtt tacagaacat atcgcgcaaa
    24481 acgccgccaa atacgccggt cgtcacagct gcgacaacgg caatgatcgg gccgtgtccc
    24541 atatccagtg caacctgtgc gccaatgata gaaaagacca ccagtccgag cgcatcgagc
    24601 accagaaaca ctttgcgcag gtaaggcatt acaggagcaa cgatagtggt gagtactgcg
    24661 gcggtagcga caataatcac atattccggg tgtttgaccc agccgagcgg atagtggccc
    24721 agcagaatat cgcgcactga ccctccgcca attgccgtgg cggtcgcaat aataattacg
    24781 ccaaatgtat ccatgcgccg tcgtccggcc gccagcgccc ccgtcatggc ttcggcagta
    24841 atgccaacca aatacaaaat gtgtaacagc atggttatct ccctaaaaat ctgtctggca
    24901 gggtagcgat ttgtacgcat tgtcacgatt gagatttttt aaggtgagag gaaattgata
    24961 atttttagtt atgtgaaatc gttgttttct gtagcgtaga tgcattcttc tgacttcttg
    25021 tctgagaaaa tacataagaa aaagtaggtt aaaactgagg ggtggaaaag aaaggtgcaa
    25081 tgttagccat tgtgttcacc gcacatggac tttggcttac gcatcgggta tctcaatcaa
    25141 caaaaagagt ttttagtttc ggctattttt tctctgtaat ttttacactg ttttccaact
    25201 gcttgatgct gatctgcggg gtcggcaact cctcaggcat ggttccgcca agttcctgaa
    25261 tggtttgcct caccttgcga cccacgtcaa agtgtgtggt gttagcttgc tgttttgaat
    25321 tcacgccatc ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga ttagccgcca
    25381 gttctgttga acccatatga tccaggatct tctgactctt tttcagcccc ttccgctgat
    25441 ggatggcttt ctgatctaat ccaccataca gcccccggta accatgattc tgaaatatgg
    25501 cgaaatcgat agctgtcgct accccagctt gctgcgctgc ctcaactaat tgtttgttat
    25561 gttctttcaa ctcatttctc agaaaaagac gcttttcgtc ttcacgaagt tgcctgaaag
    25621 cttcatcgtc agccagctcc tgtcgtcggg tctggatagc aaaataagtt tgccctgccg
    25681 caataaccgg tttcgcaggg tcgccgtttt gtactaccag gtaacaggca tagcgggaga
    25741 gtacgatgtc ttttaatgct cttttggcat ttgagccaat tttgaccata tcgaggatct
    25801 cctcgaaatg gtcatcaatt gtgtgaccac tattcagaca agcttctttg gcgcgcgtta
    25861 atacaggtat aaagtgacga tactctgaat aacccagtag tttcccaagt tgtcttgctg
    25921 accattgttc tgctccgttt gcattaatca gctttatctc ttcaaaaggt tgatgatgtt
    25981 cgttcattgc ccactccatg tgatatcgat tgcaaggaaa gtaagtctac aactgtgttt
    26041 atatacagtt gtgagtatct cacgttctca ctgcgctggg caaaaaaaca tcgcaaaatc
    26101 tggagcaggc acgcaaaaca gaattgaatg ttgcggggaa gaaaaatgcc gctctgtttt
    26161 agtggcggca tttgtgaagg cggaaaagta agaattggcg ttacgagtta ctcgatattc
    26221 tggatctgct cgcgcatctg ctcaatcaac actttcagct caatcgcgga gtttgtcact
    26281 tcggcattga tagacttcga cgcaagagtg ttcgactcgc ggttgaattc ctgcatcata
    26341 aagtccagac gacgaccaac ggcttctttt ttcttcagaa tgttgtaggt ctctttgaca
    26401 tgcgcttcta ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccaac
    26461 tcctgctcca gacggttgtt ttccaactga acctgagcat cttccagctt cgcgaccaga
    26521 cgctcacgct gccattgcag gatttccggc atatgggcgc ggactttgac cacttcggcg
    26581 gtgacgcctt ccagacgctg ctcgatcaat gctttcagcg cctgaccttc ggtttcgcgc
    26641 gcgacgataa agtcgtccag cgtaccatcc aacgccgcga gaatttcagc ggcaatcgcg
    26701 tcgagatcct gctcctgggc tgccatcacg cccggccagc gtagaatatc aaccgggttg
    26761 atttcccctt cgtcactctg catttttacc cagttcgcgg cagttaccag ctgtttagcc
    26821 agtttttcgt tgaggatcag ctcaccttgt gcgctaacat ctggttcata gcgcagggta
    26881 cattccactt taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac aggttcaagg
    26941 ctacggaact gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc
    27001 atttcccagg ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata
    27061 ctgcggatca tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat
    27121 gccttgtcag gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc
    27181 aagccggaga tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta
    27241 ccctgactcg taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata
    27301 ccaaagtatt gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg
    27361 gccagggctg gatcaccgca gagtacggca tgctgccacg ttctacccac acccgtaacg
    27421 cgcgtgaagc ggcgaaaggt aagcagggtg gacgcaccat ggaaatccag cgtctgatcg
    27481 cccgtgctct tcgtgcggca gtagatttga aagcgctggg tgagttcact attacgctgg
    27541 actgcgacgt gcttcaggct gatggtggca cgcgtaccgc ttcgattacg ggtgcctgcg
    27601 tggcgctggc agatgcgcta cagaagctgg tggaaaacgg caagctgaaa accaacccga
    27661 tgaaagggat ggtagccgca gtttctgtcg ggattgtgaa cggcgaagcg gtttgcgatc
    27721 tggaatacgt tgaagactct gccgctgaaa cggacatgaa cgtggtgatg actgaagacg
    27781 ggcgcatcat tgaagtgcag gggacggcag aaggcgagcc gttcacccat gaagagcttc
    27841 tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg
    27901 cgctggcaaa ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag
    27961 taagatgagg agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttag
    28021 caagcaggtg ttaaagtttg gcgagtttac gctgaaatcc gggcgcaaaa gcccctattt
    28081 cttcaacgcc gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc
    28141 tgaagcattg gtggattccg gcattgagtt cgatctgctg tttggccctg cttacaaagg
    28201 gatcccgatt gccaccacaa ctgccgtggc gctggcggag catcatgacc tggacctgcc
    28261 gtactgcttt aaccgcaaag aagcaaaaga ccacggtgaa ggcggcaatc tggtcggtag
    28321 cgcgttacaa ggacgcgtaa tgctggtgga tgatgtgatc accgccggaa cggcgatccg
    28381 cgagtcgatg gaaattattc aggccaatgg cgcgacgctt gctggcgtat tgatttcgct
    28441 cgatcgtcag gaacgggggc gcggcgagat ttcggcaatt caggaagttg agcgtgatta
    28501 caactgcaaa gtgatctcta tcatcaccct gaaagacctg attgcttacc tggaagagaa
    28561 gccggaaatg gcggaacatc tggcggcggt taaggcctat cgcgaagagt tcggcgttta
    28621 aagaaactcg ccggatgaaa agtcatccgg cgtcatatta ctgcaactgt gcggcaatta
    28681 gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt aaactcgctg cggacaaagc
    28741 gtgacagcat accttcacag aaggccagga tctggcttgc cagcagggtt tcatcggtgg
    28801 tgtaaccttc accctcacgc attctctttt cgcgcaatac ctgacgcaac tgcgcttcaa
    28861 tacgctcgaa cagctggttg atgcgccctt gcaggcgatc ctgttcaaac attagtgcat
    28921 gaccagtgag gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca
    28981 caatcagacg caggcgcgct gtggtgtcct tctcatcttt cagaatcaga ttgatgcgag
    29041 taatcaggct atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga
    29101 agtggcgata cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga
    29161 tacgttggct tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc
    29221 gacggttcct tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc
    29281 acttgccagg cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac
    29341 gttattcgga tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg
    29401 tcggtggcgt cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc
    29461 atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg
    29521 gaaatcatca actggccctg atagtcagaa tcgatcaatc ctaccaggtt accaagcacg
    29581 ataccgtgct tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg
    29641 gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg
    29701 gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg
    29761 agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata
    29821 acgggtcacg atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa
    29881 gactttatct ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg
    29941 agttggctgg gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg
    30001 ccgggcgtat tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg
    30061 gtcttttagt gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa
    30121 ttcatcaccc tgcgtggcct gctttttgat tttctctggg gccacggtag ctgcgcgata
    30181 atccgccacg gcggcgcaac cgataaaaat attttgctgc tgtacagaag cattcacggc
    30241 cgcttccatt tccagcgcag tcatcacatc aacacgttta acaaacggtg gcgtcggtag
    30301 tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat
    30361 agcaaaaccc atcttgccgg agctgtgatt agagatataa cgcacgggat cgagcggttc
    30421 acgcgtcggg ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa
    30481 atgcgccacc gccatatcca caatggttaa cggatcgagc attcgcccag gaccgatatc
    30541 accacaagcc tgactgccac tgtctggccc ccagatgagc aaaccacggg aagcaagcac
    30601 ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac
    30661 ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg ccattccggc
    30721 agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt
    30781 acccagctca atatggccca tagcggcttc ggctgctggg tccagcagac tgtcggaaac
    30841 gggataacca gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt
    30901 catggctacg cggacgtcgg ccccgcgctc gcgcaaacga cgcaccagtt caggggtttt
    30961 ataggcagca ataccgccgc tgacgccgag aacgattttt ttaccggcca ggctcatgat
    31021 gatttttcct gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg
    31081 tgccttcacc tgagaatcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat
    31141 ttaacggaac ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat
    31201 gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc cttaacggat
    31261 gtcgagctgc tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg
    31321 gcaaaagaga tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgaa
    31381 caatttagtg gtgttcatgg aattggcgtg gcgaaatttg cccagttaaa ggggattgct
    31441 gaactagcgc ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt actcagcccg
    31501 gagatgacgc gggaattttt acaaagccag ctcacggggg aggagcggga gatctttatg
    31561 gtgatctttc tcgactcaca acaccgggtt ataacgcata gccgtctttt ttccggcacg
    31621 ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaatgcc
    31681 tcggcgctga tccttgcaca taatcaccct tcgggttgtg ctgaacccag taaagcggat
    31741 aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac
    31801 catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc
    31861 gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag
    31921 cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt
    31981 tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga
    32041 agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc
    32101 aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc
    32161 ctgccgaacc tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg
    32221 cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct
    32281 gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag
    32341 gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta
    32401 cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc
    32461 gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt tccgctttga tgtaacaaaa
    32521 aacctcgctc cggcggggtt ttttgttatc tgcttgcccc catattgact gcatctgttc
    32581 attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa
    32641 ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc gcaacggacg cttgcgctgg
    32701 ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcggcgg
    32761 gctaaatatc tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct
    32821 ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca tgttgatttg
    32881 gtgatgagca acggtaaagt gctgcgctac accgatccgc gccgctttgg tgcctggctg
    32941 tggaccaaag agctggaagg gcacaacgtg ctggcccatc ttggaccgga gccgcttagc
    33001 gacgatttca acggtgagta cctgcatcag aagtgcgaga agaaaaaaac ggcgattaaa
    33061 ccgtggctga tggataacaa gctggtggta ggggtaggga atatctatgc cagcgaatct
    33121 ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcagagtgt
    33181 gaattgctgg ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggcggtaca
    33241 acgctgaaag attttctgca aagcgacggt aaaccgggct atttcgctca agaattgcag
    33301 gtttacgggc gaaaaggcga gccgtgtcgt gtgtgcggta cgccgattgt ggcgactaaa
    33361 catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattgat gcgcgccgga
    33421 tggcatacca tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc
    33481 tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa
    33541 gagataaacg accactcttt cgacggcatc agaaacacac tttccagttc cggcattaag
    33601 tggcgattca tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca
    33661 cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg
    33721 accacttcca cgttccccag atgcgcggtt gcctgctgtg ccagcgccac acgctcttcc
    33781 agagtaaaca tcggtttttt actggggctg gcggcaatcg ccagaataac gtgatcgaac
    33841 atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc
    33901 ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc
    33961 cagcagttga agcaggcgct gtagcgcgcc ctggttttga tacagtactt caacggcatg
    34021 acggccatag aaactacggt aatcggcatc ggtgagtaaa gaggaaacct cttttgcaag
    34081 cgtagtggca tcggtaacgg taatcagccc gcttgcctgt tccagccgcg cgcaaatgtc
    34141 tttaaagtta aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag
    34201 cggattatgc ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc
    34261 gtacagtaac atcagctcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaagg
    34321 gacttcccct gaagagcgtg tgatatagct tagtccagcc tggcggacaa ggttaatcgc
    34381 atccgggaag cgttccggat gacggggtac caggatgagc aataaattag ggaattgctg
    34441 taacaatgcc tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag tgctggtggc
    34501 aatccatacc gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt tagcagccag
    34561 ttgcggcgtt acagaaatat caaatttcag gctaccggta acggtcacct gattattttt
    34621 tgcgcccagc gccacaaaac gcgcgccatc ttcttcattt tgcgcagcaa tcagtgtaat
    34681 acgacgcagc aagcggcgga cgaatttacc cagtttggcg taacctgcgg ccgagcgggc
    34741 agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag
    34801 gttaggccat agttcggttt ccataatcaa caccagttta gggtcgactt tattcaggaa
    34861 acgattaagt gcatcgggca ggtcatacgg cagataaacg tgctgaacat ccttcccgaa
    34921 agccgattgt acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa tcggtaaatc
    34981 aggataacga tgacgcagcg cgcgcaccaa cgggattgcc gccagagttt caccgacgga
    35041 gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc
    35101 accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat
    35161 cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg
    35221 acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg
    35281 tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt
    35341 atttttatgc gattcattga tttctgcagt acgtgcagga aggaatcgca gagcaacagt
    35401 gaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg
    35461 tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt gatacactag cgcgtcaata
    35521 tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg
    35581 cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag
    35641 aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag
    35701 ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat
    35761 cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc
    35821 aaaagcctca gaaaaaatcg ccaacttttt tcatctcatc aaggtattac gtgccaataa
    35881 gtatgacctt atcgtcaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt
    35941 aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa
    36001 aagtttcacc catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt
    36061 gctgacacca ttgggacttg attcgttggt gaagcagaca accatgagtt acccgcctgc
    36121 aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt
    36181 tatccaacct acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt
    36241 gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga
    36301 cgatctggtc tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct
    36361 ggccggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat
    36421 tggcgttgat tccgcaccgg cgcatattgc cgcagcagtt aatacgccgc tgatatcgct
    36481 gtttggtgcg acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg
    36541 ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct
    36601 ttcggttatt ccggcggcag atgtcattgc tgctgtcgat aaattactgc cctcctccac
    36661 gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt
    36721 ttgcagcgcg attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg
    36781 gtttataccc agtcgtggga aggcgaatgt cctgatgtat ttgaactgat caaagtgccg
    36841 gttaaatcgc ataccaatca cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat
    36901 ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca aaatgccggg gctggacgtt
    36961 tattatgccg ctgatgtttg ttatgccgag aaagtagcgc aggaaaaagg ctttttctat
    37021 cgcctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggc
    37081 aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat
    37141 cagactgaag tggagcgttt tcatattctg ccaccgggga tttatcctga tcgtaaatat
    37201 agccagcagc cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa
    37261 caatatttat tgttgcaggt tggttcagac ttcacgcgta aaggtgtcga tcgttccatt
    37321 gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattgctata tgttgttggg
    37381 caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt
    37441 cacttcttct cggggcgcaa cgatgtctcg gaattaatgg cggcggcgga tttattactg
    37501 catcctgcct accaggaagc cgcgggaatt gtactgctgg aagcgataac tgcaggatta
    37561 ccggtactaa caacagccgt ttgtggctat gcgcattata ttgtcgacgc caattgcggc
    37621 gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta
    37681 acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa
    37741 gatttataca gtctgccaga gaaagcggcg gacatcataa cgggtggtct ggatggttga
    37801 acttaaagag ccgtttgcca cgttatggcg cggtaaagat ccttttgagg aagttaaaac
    37861 cttgcagggt gaggtatttc gtgaactgga aactcgccgc actctgcgct ttgaaatggc
    37921 gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa
    37981 tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg
    38041 actgcgggat gtcggcgttg atactatgta tggggtggca ttcggcgaaa aaggcattaa
    38101 tccgctcacc agaacctcgt ttattataac cgaagatctg acaccaacca tcagtctgga
    38161 agattactgt gctgactggg cgactaaccc accagatgtt cgcgtaaagc gtatgcttat
    38221 taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg
    38281 ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat
    38341 ttcagtaatt gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc gttggcgcga
    38401 taaagatctt attggacttt atttttcttc gatgaatatt ggcctgactc agcgggatat
    38461 ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa gacattatca agcaggaaca
    38521 agggctgctg tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattagaaa
    38581 atcgttgtaa tttccggaga gaaatatgag tgcccactat tttaatccac aagagatgat
    38641 caataagaca atcatcttcg atgaaaggcc agcggcgtca gtggcatcat cattccatgt
    38701 ttcttatggc attgataaaa actttctttt tggttgtggt gtttcaatca cgtcagtttt
    38761 gttacataac aacgacgtga gttttgtttt ccacgttttt attgatgcta tccctgaagc
    38821 cgatatccag cgtttagccc aattggcgaa aagctatcgt acctgtatcc agatccatct
    38881 agtaaattgt gaacggctta aggcattacc gacgaccaaa aattggtcta ttgccatgta
    38941 tttccgtttt gtaattgcag attactttat tgatcaacaa gataagatcc tttacctgga
    39001 tgctgatatc gcctgtcagg gaaacttaaa gccgctgata acaatggatc ttgccaataa
    39061 cgttgctgct gttgttactg aacgcgatgc taactggtgg tcgttacggg gtcaaagtct
    39121 gcagtgtaat gaacttgaaa agggttactt taattcaggt gtcctgttaa ttaatacact
    39181 agcgtgggcg caggagtccg tttctgctaa agcgatgtcg atgcttgctg ataaagccat
    39241 cgtttcccgt ttaacctata tggatcaaga tatccttaat cttatcctgt tagggaaagt
    39301 taaattcatt gatgctaaat acaatacgca atttagttta aattatgaat taaaaaaatc
    39361 atttgtttgt ccaattaatg atgaaaccgt attaattcat tatgtcggcc cgacaaaacc
    39421 ctggcattac tgggccggtt atccaagtgc gcaacctttt atcaaagcca aagaagcatc
    39481 gccctggaaa aatgaaccgt taatgcggcc agttaactca aactat
//
LOCUS       DARNVM010000021        38869 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.021, whole genome shotgun sequence.
ACCESSION   DARNVM010000021 DARNVM010000000
VERSION     DARNVM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.021"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..675
                     /locus_tag="SI621_001071"
     CDS             <1..675
                     /locus_tag="SI621_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063812.1"
                     /translation="AMDKFLISFSLKDNGAEYIMTLRGSGFEYEEIPITINEYNSFMD
                     FKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQAFGDVSLGYAVLDDIVSKLKR
                     GKFDLQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLE
                     DEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITEEKICEKYKSTIEAFKKK
                     QLALPV"
     gene            complement(765..1319)
                     /locus_tag="SI621_001072"
     CDS             complement(765..1319)
                     /locus_tag="SI621_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HEI4063813.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMGLLQKLLK"
     gene            1644..1850
                     /locus_tag="SI621_001073"
     CDS             1644..1850
                     /locus_tag="SI621_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HEI4063814.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1912..3255)
                     /locus_tag="SI621_001074"
     CDS             complement(1912..3255)
                     /locus_tag="SI621_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEI4063815.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3578..4216)
                     /gene="msrA"
                     /locus_tag="SI621_001075"
     CDS             complement(3578..4216)
                     /gene="msrA"
                     /locus_tag="SI621_001075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEI4063816.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4422..6155
                     /gene="tamA"
                     /locus_tag="SI621_001076"
     CDS             4422..6155
                     /gene="tamA"
                     /locus_tag="SI621_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HEI4063817.1"
                     /translation="MRYIRQLCCVSLLCLSGAAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6152..9931
                     /gene="tamB"
                     /locus_tag="SI621_001077"
     CDS             6152..9931
                     /gene="tamB"
                     /locus_tag="SI621_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HEI4063818.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLDCLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQIQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9934..10275
                     /locus_tag="SI621_001078"
     CDS             9934..10275
                     /locus_tag="SI621_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEI4063819.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIGNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10487..10738
                     /gene="chpS"
                     /locus_tag="SI621_001079"
     CDS             10487..10738
                     /gene="chpS"
                     /locus_tag="SI621_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HEI4063820.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            10732..11082
                     /gene="chpB"
                     /locus_tag="SI621_001080"
     CDS             10732..11082
                     /gene="chpB"
                     /locus_tag="SI621_001080"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HEI4063821.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVWMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(11286..11816)
                     /gene="ppa"
                     /locus_tag="SI621_001081"
     CDS             complement(11286..11816)
                     /gene="ppa"
                     /locus_tag="SI621_001081"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEI4063822.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12126..13082
                     /gene="ytfQ"
                     /locus_tag="SI621_001082"
     CDS             12126..13082
                     /gene="ytfQ"
                     /locus_tag="SI621_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HEI4063823.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13214..14716
                     /locus_tag="SI621_001083"
     CDS             13214..14716
                     /locus_tag="SI621_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEI4063824.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIDREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTL
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAQRFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14730..15752
                     /locus_tag="SI621_001084"
     CDS             14730..15752
                     /locus_tag="SI621_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063825.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            15739..16734
                     /gene="yjfF"
                     /locus_tag="SI621_001085"
     CDS             15739..16734
                     /gene="yjfF"
                     /locus_tag="SI621_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HEI4063826.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16767..17765)
                     /gene="fbp"
                     /locus_tag="SI621_001086"
     CDS             complement(16767..17765)
                     /gene="fbp"
                     /locus_tag="SI621_001086"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEI4063827.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            17941..19314
                     /gene="mpl"
                     /locus_tag="SI621_001087"
     CDS             17941..19314
                     /gene="mpl"
                     /locus_tag="SI621_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEI4063828.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDALADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19464..20015)
                     /locus_tag="SI621_001088"
     CDS             complement(19464..20015)
                     /locus_tag="SI621_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEI4063829.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20109..21461
                     /gene="pmbA"
                     /locus_tag="SI621_001089"
     CDS             20109..21461
                     /gene="pmbA"
                     /locus_tag="SI621_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEI4063830.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21644..22030
                     /gene="cybC"
                     /locus_tag="SI621_001090"
     CDS             21644..22030
                     /gene="cybC"
                     /locus_tag="SI621_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HEI4063831.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            22222..22464
                     /locus_tag="SI621_001091"
     CDS             22222..22464
                     /locus_tag="SI621_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEI4063832.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            22454..22744
                     /locus_tag="SI621_001092"
     CDS             22454..22744
                     /locus_tag="SI621_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4063833.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGKRENSAVYEDATKRLDE"
     gene            complement(22745..23209)
                     /gene="nrdG"
                     /locus_tag="SI621_001093"
     CDS             complement(22745..23209)
                     /gene="nrdG"
                     /locus_tag="SI621_001093"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HEI4063834.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(23389..25527)
                     /gene="nrdD"
                     /locus_tag="SI621_001094"
     CDS             complement(23389..25527)
                     /gene="nrdD"
                     /locus_tag="SI621_001094"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEI4063835.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25921..27576)
                     /gene="treC"
                     /locus_tag="SI621_001095"
     CDS             complement(25921..27576)
                     /gene="treC"
                     /locus_tag="SI621_001095"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEI4063836.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRVLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27626..29047)
                     /gene="treB"
                     /locus_tag="SI621_001096"
     CDS             complement(27626..29047)
                     /gene="treB"
                     /locus_tag="SI621_001096"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HEI4063837.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(29166..30113)
                     /gene="treR"
                     /locus_tag="SI621_001097"
     CDS             complement(29166..30113)
                     /gene="treR"
                     /locus_tag="SI621_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HEI4063838.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKVHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIETLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            30298..30351
                     /gene="mgtL"
                     /locus_tag="SI621_001098"
     CDS             30298..30351
                     /gene="mgtL"
                     /locus_tag="SI621_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HEI4063839.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            30492..33188
                     /gene="mgtA"
                     /locus_tag="SI621_001099"
     CDS             30492..33188
                     /gene="mgtA"
                     /locus_tag="SI621_001099"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEI4063840.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIQIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLTGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(33394..33780)
                     /gene="ridA"
                     /locus_tag="SI621_001100"
     CDS             complement(33394..33780)
                     /gene="ridA"
                     /locus_tag="SI621_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HEI4063841.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33853..34314)
                     /gene="pyrI"
                     /locus_tag="SI621_001101"
     CDS             complement(33853..34314)
                     /gene="pyrI"
                     /locus_tag="SI621_001101"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEI4063842.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(34327..35262)
                     /gene="pyrB"
                     /locus_tag="SI621_001102"
     CDS             complement(34327..35262)
                     /gene="pyrB"
                     /locus_tag="SI621_001102"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEI4063843.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(35266..35400)
                     /gene="pyrL"
                     /locus_tag="SI621_001103"
     CDS             complement(35266..35400)
                     /gene="pyrL"
                     /locus_tag="SI621_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HEI4063844.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35681..36076)
                     /locus_tag="SI621_001104"
     CDS             complement(35681..36076)
                     /locus_tag="SI621_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEI4063845.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPAQ"
     gene            complement(36207..36920)
                     /gene="bdcA"
                     /locus_tag="SI621_001105"
     CDS             complement(36207..36920)
                     /gene="bdcA"
                     /locus_tag="SI621_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4063846.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36991..37584
                     /locus_tag="SI621_001106"
     CDS             36991..37584
                     /locus_tag="SI621_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEI4063847.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLIKE"
     gene            37729..38181
                     /gene="tabA"
                     /locus_tag="SI621_001107"
     CDS             37729..38181
                     /gene="tabA"
                     /locus_tag="SI621_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HEI4063848.1"
                     /translation="MIIGNIHNLQSWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            38304..>38869
                     /locus_tag="SI621_001108"
     CDS             38304..>38869
                     /locus_tag="SI621_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEI4063849.1"
                     /translation="MSKISGWNFSQHITSADNFKQKSEVINTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIP"
BASE COUNT         9451 a         9607 c        10415 g         9396 t
ORIGIN      
        1 gccatggata agtttcttat cagtttcagc cttaaagata acggtgccga atatataatg
       61 acattgcgag gcagtggatt tgaatacgaa gaaattccca taacaataaa tgagtataac
      121 tcattcatgg atttcaaaaa ccgcgagttt ccgttagaac aaaacagaag attgtatgcc
      181 tgggatattc tgcagaaaaa acagtctgac ataccggaaa ggattaaagg ttatattcgc
      241 caggcgtttg gtgacgtgtc tttaggttat gctgtattgg atgatatagt aagtaaatta
      301 aaacggggta aatttgacct ccaaatacca ggaggtggaa ttaaagaatg cgatggatgg
      361 tatatttacg aaaaaataat tgatgataat tttgccatag tcatagaatc tttgggcttt
      421 gcgcttaaaa tctatggagg ggatgaacgt tttcgtaatg gttcctctgt tgttttggag
      481 gatgaagatt actctcttat ttataatttt ctggttaatg caggttgcca acaagtcgaa
      541 ttagccgaac aagtggatgc gatagtaagt gcgaatctgg ccgcagatag tgatattacc
      601 gaagaaaaga tttgcgagaa atataaatcg accattgagg cgtttaaaaa aaagcaacta
      661 gcgttaccag tatgagttcg gcgtaagaac tcagaggctt aattccccta tcccatagat
      721 aacgataggg gaaaattatt tttgcagaat ttatgttatt gccattattt gagcaacttt
      781 tgcagcaggc ccatcacttg ctgtacctct tccggtgtga gcgccccgtc tttggcccat
      841 tgcacgcgac cgtctttatc cagcaccact acagcggaac tttcttcatc cagctgccag
      901 gcaccgcgcg cgacgccatt gctatcgaca ataaactgcg accagggata aagcttttta
      961 ttactctcca gactgctgcg cacaaacatg ccggaacccg gaattgcgtc gtcggtgtta
     1021 acaatggtgg tggtctggta acgatcgtgc ggtaactttg ctgatttaat cgcttcaatc
     1081 agcgtcgcgt ttttctcttt tgcagaggtg cgaccagcaa tatgttgcag tactcgcact
     1141 tttcccacta actgcgcgct gttccaggtt ttgtagctaa actgatcttt atcaagcacc
     1201 aactcgcccc gatcggtaat gccaatcggc ggcactcgct gaccggtttc gaactgatgt
     1261 gcggaagcca tcatcggcaa cagcaggcag gtgagtgcca gaatcttgcg tagggtcatg
     1321 gtgtttcctt cttatgatat gcaggtgatc cgaccacttg ggtctgagtt ttaatcatat
     1381 gtgctattta tcgaatttcc cgcaagtgtg atgccagttt gcggtcaagc gcacaaatca
     1441 tatgaaaaat gaatgcttat actgaagacc acgcttcggt aaaaagataa ttctgaataa
     1501 ttgtaacctt taggtaaaaa aagttatacg cggtggaaac attgcccgga tagtctatag
     1561 tcactaagca ttaaaatttg cgcctcataa tagttgggcc gattgtggca ccgcacaggc
     1621 gtaatactca gcaggagata acaatgaaaa ttttccaacg gtacaaccca cttcaggtgg
     1681 cgaagtacgt aaagatcctg ttccgtggac ggttatacat caaggacgtt ggcgcttttg
     1741 aatttgataa gggtaagatt cttatcccaa aagtgaagga taagctgcat ttgtcagtga
     1801 tgtccgaagt taaccgtcag gttatgcgtc tgcaaacaga gatggcttaa ccaaagtgct
     1861 atgcagtaat aagacggctc ctgattcagg agccgttgat gtttctgggg gttacgcgac
     1921 gctttcttct ttatctttag cgtcaggcag ttttggcgaa agggcggtgg ccttgctgtc
     1981 gattcgggtc accagcagct ggtcgatgcg gtagttatcg atatccacca cttcaaattt
     2041 gtagccggcg aatttcaccg aatcggtgcg tttcgggatc ttacgcagca taaacatcat
     2101 aaagccgccg atggtttcgt agttgcccga ctgcgggaac tcgtcaatat ccagcacgcg
     2161 catgacgtcg tcaatcggag tgccgccgtc aatcagccat gagttctcat cacgggcgac
     2221 aatctgctct tccagcccct gaccgaccag atcgcccatc agcgtggtca tcacgtcgtt
     2281 gagggtgatg atccccacta ccagcgcgta ctcgttcatg atcaccgcga agtcttcacc
     2341 tgcggtttta aaactttcca acgcctctga aagggttaac gtatccggca caatcagcgt
     2401 gttgcggatt tgtacgccgc tgttcagtgc caggctttgg ttagccagca cgcggttcag
     2461 caggtcttta gaatcgacat agccgatgat gtggtcaata tcttcattac agacgaggaa
     2521 cttagagtgc ggatgttccg ccaccttatt cttcagactt tgctcatctt cgtggagatc
     2581 aaaccaaatc acgttttcac gcggtgtcat tgacgacgga acggtacggg attccagctc
     2641 aaagacgttt tcaatcagct cgtgttcctg tttacgtaac acgcccgcca gcgcaccagc
     2701 ttccactacc gcgtagatgt catcagaagt gatgtcatct ttacgtacca ttggcagttt
     2761 gaaaatacgg aagatcatgt tcgccagtcc gttgaagaac cacaccagcg gggtgcaaac
     2821 gtacaggcag aagcgcatcg ggttgatgat acgcaaagcc acagcttctg gcgcaatcat
     2881 accgatgcgt ttcggggtta aatccgcaaa caggataaac atgccagtca ctaacgagaa
     2941 agagagaata aagctcagtt gctcagagag ttctgccgac atatagcggg agaacaggct
     3001 gtgaaaagct ggagaaaatg ccgcatcacc gacgataccg ccgagaatcg ccactgcgtt
     3061 cagaccgatt tggaccacgg taaagaacat gccgggattt tcctgcatat tcagaacgcg
     3121 ttgggcattt atattgcctt catcagccag cagtttaagt ttgattttgc gtgaagcggc
     3181 aagcgagatc tcggacatcg agaagaacgc acttacagcg atcaagcaga gtatgactaa
     3241 aatactgttt aacatatctt atccgacctt tcaggccaga tcctcggaag gggaagttga
     3301 ttaatttgtg tgaaatacac attgaaagcc gattcgaaga aagtgaaccg gcattttcag
     3361 tggctagtat agcgtaaggt actgtaaagc cgccagaggg ttaaaattca gactgctgat
     3421 aaatgaaaaa ctgcctgatg cgctatgccg atcaggccta cgcagtcccc acaatatatt
     3481 gattttggac gattttgtag gcctggcaag gcgtttacgc cgcatccggc atagacaaat
     3541 agcactttat caacaatcta catttgtacc cgtaacgcta tgcttccggc ggcagacaga
     3601 cgccaattcc gccaattcca cagtaaccat acgggttttt atgcagatat tgctggtggt
     3661 catcttcggc ataataaaac ggtgtggcgt tcgcgatttc cgtggtgatg tgacgatcgt
     3721 catcggcggc aagcatcgcc gcctgaaaac gttccagact ggcgcgagca gcagcatcct
     3781 gttctggggt cagtggataa atcgctgaac gatactgcgt gccgtggtca ttgccctgac
     3841 gcattccctg ggcgggatcg tgattctccc aaaatacttg tagcaactgt tcatagctga
     3901 tgacggaagg atcgtaaaca atgcgtaccg cttcggcatg acccgtatca ccggagcaca
     3961 cttcccgata agtcggattt ggcgtatagc cgccggtata gcctgcggcg gtgctgtaaa
     4021 cgccgggtaa ctgccagaac agacgctcca caccccagaa acaacccatc gcaaaaatgg
     4081 caatctccat tccgtcaggt acattggtca ttgagtgacc gttgaccgca tgcagtgtgg
     4141 ctacgggcat cggggtgtta cgtccaggca gggcatcggc gggggaaacc agatgctttt
     4201 tatcaaataa actcatggtg tcgctctccc gaaaatcggt cattggggtt aaggttgtaa
     4261 caagaggcgt atttgcacac aataaccgct gtgaataggt ctaaagtaaa acataagaaa
     4321 tatttggggt ttagtctgct ttttaatcca tattactgga tttttgttaa gccgtttaac
     4381 ggcgttccag gggcaggaaa aaaggatatt caggagaaaa tgtgcgctat atccgacagt
     4441 tatgctgtgt aagcttactc tgcttaagcg gagctgccgt cgccgcgaac gtccgtctac
     4501 aggtcgaggg gttatcggga cagctggaaa agaacgttcg tgcgcagctt tctacgattg
     4561 aaagtgatga agtgacgcca gaccgtcgct ttcgcgcacg cgtcgatgat gccatccgcg
     4621 aaggtctgaa agcgctgggt tattaccagc cgaccattga atttgatctc cgtccaccgc
     4681 caaagaaagg gcggcaggta ttgatcgcca aagtcacgcc aggcgtgccg gtgttaattg
     4741 gcggcaccga tgtggtattg cgcggcggcg cgcggaccga taaagactat ttgaaattgc
     4801 tcgatactcg cccggctatt ggtacggtac tgaaccaggg cgattatgaa aatttcaaaa
     4861 agtccttaac cagcattgcg ttgcgtaaag gttatttcga tagcgaattt accaaagcgc
     4921 agctgggcat tgcgctcggc ctgcataaag ccttctggga tattgattat aacagtggcg
     4981 aacgttaccg ctttgggcat gtgacctttg aaggatcaca aatccgcgat gaatacctgc
     5041 aaaatctggt gccgtttaaa gagggcgatg agtacgaatc gaaagatctg gcggaactga
     5101 accgtcgact ttctgctacc ggctggttta attcggtggt ggtggcacca caatttgata
     5161 aagcgcgcga aacgaaagta ttaccattga cgggcgtggt ttcgccgcga actgaaaaca
     5221 ccatcgaaac cggggtcggt tactctacgg acgtagggcc acgtgttaag gcaacgtgga
     5281 aaaaaccatg gatgaactcg tacggtcata gcctgaccac cagtaccagt atttccgcgc
     5341 cggaacagac cctcgacttc agctataaaa tgccactgct gaagaatcca ctggaacaat
     5401 attatttggt gcagggcggt tttaagcgca ctgacctgaa cgataccgag tctgactcca
     5461 ctacgctggt ggcttctcgc tactgggatc tttccagcgg ctggcagcgt gccattaacc
     5521 tgcgctggag tctcgaccac tttacccaag gtgaaattac caacaccacg atgctgtttt
     5581 atcctggggt gatgattagc cgcacgcgtt ctcgcggtgg cctgatgcca acctggggcg
     5641 actcgcaacg ctactctatc gactactcca acacggcctg gggctcagat gtcgatttct
     5701 ccgttttcca ggcgcagaac gtctggatcc gcacactgta cgatcgccat cgttttgtta
     5761 cacgtggcac gctgggctgg attgaaaccg gtgatttcga caaagtaccg ccggatctgc
     5821 gtttcttcgc cgggggcgat cgcagtattc gcggctacaa atacaaatca atcgctccga
     5881 aatacgccaa cggtgacctg aaaggggcct cgaagttgat aaccggatcg ctggagtacc
     5941 agtacaacgt gaccggaaaa tggtggggcg cggtgtttgt cgatagcggc gaagcggtaa
     6001 gcgatattcg ccgcagcgac tttaaaaccg gtaccggggt cggcgtgcgc tgggaatcgc
     6061 cggtcgggcc aatcaaactc gattttgccg taccggtcgc ggataaagac gaacacgggt
     6121 tacagtttta catcggtctg gggccagaat tatgagttta tggaaaaaaa tcagcctcgg
     6181 cgtggttatc gttatcttac tgttgctggg atcggtggcg tttctggtgg gcaccaccag
     6241 cggcctgcat ctggtattta aagcggcgga tcgctgggtg ccaggactgg atattggcaa
     6301 agtcaccggc ggctggcgcg atctcacctt gtctgacgtt cgttatgagc agccaggcgt
     6361 ggcggtaaaa gcgggcaatc tgcatctggc ggttgggctg gattgtcttt ggaacagtag
     6421 cgtttgtatt aatgacctgg cgctgaaaga cattcaggtc aacatcgaca gtaaaaaaat
     6481 gcctccttct gaacaggttg aagaagagga agatagcggc ccgctggatc tctccacgcc
     6541 atatcccatc accctgacac gggtggcgct ggataacgtc aacatcaaga ttgatgacac
     6601 cacggtatcg gtgatggact tcacctccgg cctgaactgg caggagaaaa ccctgaccct
     6661 gaaaccgacg tcgctgaaag gcctgttgat tgctttgccg aaagtggcgg aagtggcgca
     6721 ggaagaagtg gtcgaaccga agattgaaaa tccgcagccg gaggaaaagc cgctcggcga
     6781 aacgctgaaa gatctctttt ctcgcccggt attgccggaa atgaccgacg tgcatttgcc
     6841 gcttaacctg aacattgaag agtttaaggg cgagcagctg cgcgtgacgg gcgacacaga
     6901 catcaccgtg cgcaccatgc tgctgaaagt gagcagtatc gacggcaata ccaaactgga
     6961 cgcgctggat atcgactcca accaggggat tgtcaacgcc agcggcacgg cgcagctatc
     7021 agacaactgg ccggtggata tcaccctcaa cagcacactg aacgtggagc cgttgaaagg
     7081 tgaaaaggtg aagctgaaag tgggcggcgc gctgcgcgaa cagctggaga taggcgttaa
     7141 cctttctggt ccggtggata tggatttacg cgcccagacg cgactggcgg aagccggatt
     7201 gccgctcaac gtggaagtga acagcaaaca gctttactgg ccgttcactg gagagaagca
     7261 gtatcaggcg gatgatctga aactgaaact taccggcaaa atgaccgatt acacgctctc
     7321 tatgcgcacg gcagtgaagg gacaggagat cccgccagcc accattaccc ttgatgccaa
     7381 aggtaatgaa cagcaggtca atctcgacaa actcaccgtc gcggcgctgg aagggaaaac
     7441 tgaactcaaa gcgttgctcg actggcagca ggccattagt tggcgcggtg agctaacgct
     7501 taacggcatt aacaccgcca aagagttccc ggagtggccg tcgaaactca atggcttgat
     7561 taaaacccgc ggtagcctgt acggcggcac ctggcagatg gaggtgccag aactgaagct
     7621 gaccggtaac gtcaaacaga acaaagtgaa cgttgacggc acgctgaaag gcaacagtta
     7681 tatgcagtgg aagatcccag ggctgcatct ggaactgggg ccaaacagtg ccgaagtgaa
     7741 aggcgagctg ggggtaaaag atctcaatct tgatgccacc atcaacgcgc cggggctgga
     7801 taacgcgctg ccggggcttg gcggtacagc gaaagggctg gtgaaagtac gcggcacggt
     7861 ggaagcgcca caactactgg cagatatcac cgcgcgcggc ctgcgctggc aggaactttc
     7921 cgtggcgcag gttcgcgtgg aaggcgatat caaatccacc gatcagattg cgggtaaact
     7981 tgacgtacgc gttgagcaaa tctcgcagcc ggatgtaaat atcaacctcg tcactctgaa
     8041 tgccaaaggt agcgaaaaac agcacgagct acagttgcgg attcagggcg aaccggtttc
     8101 cggacagctt aatctggcag gaagttttga tcgcaaagaa gaacgctgga agggaacact
     8161 tagcaatacc cgcttccaga cgccggtcgg cccgtggtcg ctgacccgcg atattgcgct
     8221 ggattatcgc aacaaggagc aaaaaatcag catcgggcca cactgctggc ttaacccgaa
     8281 tgcggaactg tgcgtgccgc aaaccattga tgccggggct gaagggcgtg cggtggtgaa
     8341 tctcaaccgc ttcgacctgg cgatgctgaa accgtttatg ccagaaacca ctcaggccag
     8401 cggtatcttc acgggtaaag cggatgtcgc ctgggacacc acgaaagagg ggctgccgca
     8461 gggcagtatc accctttcgg ggcgtaacgt gcaggtaacg caaaccgtca acgatgcggc
     8521 gctgcccgtg gcgtttcaga cgctgaatct gacggcggag ttgcgtaaca accgtgccga
     8581 attgggctgg accatccgcc tgaccaataa cggccagttt gatggacagg tgcaggtgac
     8641 cgatccgcaa ggccgccgta atcttggtgg caacgtcaat atccgtaact tcaaccttgc
     8701 gatgataaac cctatcttta cccgtgggga aaaagctgcg gggatggtga gtgccaactt
     8761 acgtctgggt ggtgatgtgc aaagccccca gttgtttggg cagatacagg ttacgggtgt
     8821 ggatatcgac ggcaacttta tgccgtttga tatgcagccg agccagcttg cggtcaactt
     8881 taacggtatg cgctcgacgc ttgccggtac cgtgcggacc cagcagggtg agatctacct
     8941 gaacggcgac gccgactgga gccagattga aaactggcgg gcgcgggtaa cagcgaaggg
     9001 cagtaaagtg cggatcaccg tgccgccgat ggtacgaatg gatgtatcgc cagatgttgt
     9061 attcgaggct acaccaaacc tgtttaccct cgatggtcgc gtggatgtcc cgtgggcgcg
     9121 catcgtggtg cacgatctgc cggaaagtgc ggtgggcgtc tccagcgatg tggtgatgct
     9181 taacgataac ctgcaaccgg aagaggcaaa aacggcgtcg attccgatta acagcaacct
     9241 gattgtccac gtcggcaaca atgtgcgcat tgacgccttt ggcctgaaag cgcggctgac
     9301 gggcgatctt aacgtcgtac aggataaaca agggctgggc ctgaacgggc agatcaacat
     9361 ccctgaaggg cgtttccatg cctatggtca ggatctgatt gtgcgtaagg gtgagttact
     9421 gttctctggt ccgccggatc aaccgtatct taatatcgaa gctattcgta acccggatgc
     9481 tacagaagac gacgtaatcg ccggagttcg cgtcactggt ctggcggacg aaccgaaagc
     9541 ggagatcttc tctgacccgg ccatgtcgca acaagctgca ttgtcttatt tgctacgtgg
     9601 acaagggctg gagagcgatc agagcgacag tgcggcgatg acctcaatgc tgattggtct
     9661 gggggttgca caaagtggtc agattgtggg taaaatcggc gagacgtttg gcgtaagcaa
     9721 tttagcgctc gacacccagg gagtaggcga ctcctcccag gtggtggtca gcggctatgt
     9781 attgccaggt ctgcaagtga aatacggcgt gggtatattt gactctatag caacactcac
     9841 gttacgttat cgcctgatgc ctaagctata tctggaagcc gtgtctggtg tagaccaggc
     9901 actggatttg ctctatcagt tcgagtttta gcaatgcgaa tatttgtcta cggcagttta
     9961 cgccacaaac aaggcaacag tcactggatg accaatgccc agttactggg cgatttcagt
    10021 atcggtaact accagttgta tagcctgggc cactatccag gcgcagttcc ggggaacgga
    10081 acagtacacg gtgaagttta tcgtattgac aacgccacgc tggccgaact tgatgccttg
    10141 cgcaccaggg gcggtgaata cgcgcgccag ttgattcaga cgccgtacgg gagtgcatgg
    10201 atgtacgttt atcaacgacc cgtggatgga ttaaagctaa ttgaaagcgg cgactggtta
    10261 gacagggata agtaaccata cgcatacgcc accttcgggt ggcgttgttt tttgcgaaac
    10321 gactcgcatt ctgttttgta attccctcac cttttgcttt tctctccgag ccgctttcca
    10381 tatctgttaa cgcataaaaa actctgctgg cgttcacaaa tgcgcagggg taaaacgttt
    10441 cctgtagcac cgtgagttat actttgtata acttaaggag gtgcagatgc gtattaccat
    10501 aaaaagatgg gggaacagtt caggtatggt cattcctaat gttgtgatga aagaacttaa
    10561 cttacggccg gggcagagcg tggaggcgca agtgagcaac aatcaactga ttctgacacc
    10621 catctccagg cgctactcgc ttgatgaact gctggcacag tgtgacatga acgccacgga
    10681 acttagcgag caggatgtct ggggtaaatc cacccctgcg ggtgacgaaa tatggtaaag
    10741 aaaagtgaat ttgaacgggg agacattgtg ctggttggct ttgatccagc aagcggccat
    10801 gaacagcaag gtgctggtcg acctgcgctt gtgctctccg ttcaagcctt taatcaactg
    10861 ggaatgacgc tggtggcccc cattacgcag ggcggaaatt ttgcccgtta tgccggattt
    10921 agcgttcctt tacattgcga agaaggcgat gtgcacggcg tggtgctggt gaatcaggtg
    10981 tggatgatgg atctacgcgc ccggctggca aagcgtattg gtctggctgc gggtgaggtg
    11041 gtggaagagg cgttattacg tttgcaggcg gtggtggaat aaggtgtgtt tatttatcgc
    11101 gggcataaaa aacccctact aaccgaagcc cggcgttcag ggttattgcg ctatactcgt
    11161 catacttcaa gttgcatgtg ctgcgactgc gttcattcac cccagtcact tacttctgta
    11221 agctcctggg gattcactca cttgtcgcct tcctgcaagt tgaattattt agagtatata
    11281 agaacttatt tattctttgc gcgctcgaag gaggcaacga tttcagcttt agcggcttct
    11341 gcgttttccc agccttcaac tttcacccac ttgccttttt cgaggtcttt gtagtgctcg
    11401 aagaagtgag cgatctgtgc tttcagcagt tcaggcagat cgttaacgtc tttaatgtga
    11461 tcgtattctt tgctcagctt gctgtgcgga accgcaacca gtttcgcatc ttcaccggct
    11521 tcgtcggtca ttttcagaac gccaaccgga cggcaacgga tcacagaacc cggctgcagc
    11581 gggtacggag tcgggaccag tacgtcaacc ggatcaccgt ccagagacag ggtgtggttg
    11641 atgtaaccgt agttgcacgg atagaacatc gcggtggaca tgaagcggtc aacgaacagt
    11701 gcgccgctct ctttgtcgat ttcgtatttg atcggatctg cgttagccgg gatctcaata
    11761 acaacgtaga tgtcttccgg cagatcttta cccgcaggga cgttgagtaa gctcatgtct
    11821 gtttccttta aaaatatgtg gcaaacaagt gccgagtatt atagccaact cgcgccgaat
    11881 gtcttcgctt gttttcttct tcattttcct ttttagccag ttttaagatg gtttaaagag
    11941 caggaaaatc cataaccgta gccgcatttt tcatagtgag atgaaagcga ttacaaactt
    12001 gtgattaacg ttttatttac ttttttgaag tgtgatgtaa cgcaatccgt tacataacga
    12061 attgtctata gttttttcgc gaacatcttt tcaccaataa taattacccc gacgaggaca
    12121 accctatgtg gaaacgctta cttgtagtct ctgcagtctc ggcagccatg tcgtctatgg
    12181 cgttggctgc tccattaacc gttggatttt cgcaggtcgg atcggaatcc ggctggcgcg
    12241 ccgcagaaac caacgtggcg aaaagtgaag ccgaaaagcg cggaattacg ttgaaaattg
    12301 ccgatggtca gcaaaagcag gaaaaccaga ttaaagcggt acgttccttc gtcgcgcaag
    12361 gggtggatgc gatctttatc gctccagtgg tagcgaccgg ttgggagccg gtattaaaag
    12421 aggcgaaaga tgccgaaatc ccggtcttct tgcttgaccg ttccatcgat gtgaaagaca
    12481 aatctctcta tatgaccact gtcaccgccg acaacatcct cgaaggcaag ttgattggtg
    12541 actggctggt aaaagaagtg aatggcaaac catgcaacgt ggtggagctg cagggcaccg
    12601 ttggagccag cgtcgccatt gaccgtaaga aaggctttgc cgaagccatt aagaatgcgc
    12661 caaatatcaa aattatccgc tcgcagtcag gtgacttcac ccgcagcaaa ggcaaagaag
    12721 tgatggagag ctttatcaaa gcagaaaaca acggcaaaaa catctgcatg gtttacgccc
    12781 ataacgatga catggtgatt ggcgcaattc aggcaattaa agaagcgggc ctgaagccgg
    12841 gtaaagatat cctcacgggt tccattgatg gcgtaccgga tatctacaaa gcgatgattg
    12901 atggcgaagc gaacgccagt gttgaactga cgccgaacat ggcaggtcct gctttcgacg
    12961 cgctggagaa atacaaaaaa gacggcacca tgcctgaaaa gctgaccctg accaagtcca
    13021 ccctttacct gcctgatacc gcaaaagaag agttagagaa gaagaaaaac atggggtatt
    13081 gaggggcatc aatgcctaat gccgacttgt gggccgccgg ataaggattc acgccgcatc
    13141 cggcggttgt gcgagatgcc tgatgcgacg ctgttgtgtc ttatctggcg cacgctttac
    13201 aacgggggaa actatgaata ctaaccaaca ccaggaaatc ctccgcaccg aaggattaag
    13261 taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc gccgtggtga
    13321 aatcatggcg ctgctcggtg aaaacggggc gggaaaatca acgctaatca aagccttaac
    13381 cggtgtctac cacgccgatc gtggcaccat ctggctggaa ggccagacta tctcaccgaa
    13441 aaataccgcc cacgcacaac aactcggcat cggcaccgtc tatcaggaag tcaacctgct
    13501 acccaatatg tcggtcgctg ataatctatt catagatcgc gaacccaaac gtttcggcct
    13561 tctacgccgt aaagagatgg aaaagcgcgc caccgaactg atggcatctt acggtttctc
    13621 cctcgacgtg cgcgaaccgc tcaaccgctt ttcagtggcg atgcagcaaa tcgtcgccat
    13681 ttgccgggct atcgatctct ctgccaaagt gctgatcctc gatgaaccca ctgccagtct
    13741 cgacactcag gaagtagagt tactgtttgg cctgatgcgt cagttgcgcg atcgcggcgt
    13801 cagcctgatc ttcgttactc actttctcga tcaggtctat caggtcagcg atcggatcac
    13861 cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacc cgcgagctac cgcagatcga
    13921 actggtaaaa atgatgctgg ggcgcgagct ggacacccac gcgctacagc gtgccggacg
    13981 aacattgctg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa aaggaacgct
    14041 cgcaccgttt gatctcgaag tacgccccgg cgagatcgtt ggcctggcag gcttgctggg
    14101 atcaggacgt accgaaaccg ccgaagtgat cttcggtatc aaaccagctg acagcggcac
    14161 ggcgttgatc aaaggcaaac agcaaaccct gcgatcgcca catcaggctt cggtactggg
    14221 tatcggcttc tgcccggaag acaggaaaac cgatggcatc atcgccgccg cctcggtgcg
    14281 ggaaaatatc atcctcgctc tccaggccca gcgcggctgg ctacgaccga tttcccgcaa
    14341 agaacagcaa gagattgccc aacgctttat ccgccagctt ggcattcgca caccttcaac
    14401 tgaacaaccg attgaatttc tctccggcgg caatcagcaa aaagtgttgc tttcacgttg
    14461 gctactgacc cgaccgcaat ttctgatcct cgacgagcca acgcgcggaa ttgatgttgg
    14521 tgcccacgcc gagatcatcc gcctgattga aacgctgtgc gccgatggac tggcgctgct
    14581 ggtgatctcc tccgagctgg aagagctggt gggctatgcc gaccgggtga ttatcatgcg
    14641 cgatcgcaaa caggtagcgg agatcccgct ggcagagctt tccgttccgg cgatcatgaa
    14701 cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tctcccggac actacgccgc
    14761 cgaaaaggcg ctttcgctgg ccaacaggaa tgccgcagct ggtagcacta ttgctggtgc
    14821 tgctggtcga cagcctggta gccccgcatt tctggcaggt ggtgcttcag gatgggcgtt
    14881 tgttcggtag ccctatcgac attcttaacc gtgcggcccc cgttgcgcta ctggcgattg
    14941 gcatgacgct ggttatcgcc actggtggga ttgatctctc cgtaggggcg gtgatggcta
    15001 tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg attgttttgt
    15061 taagcgccct gggcactggc atcctggcgg gattgtggaa cggcatactg gtagcgatcc
    15121 tcaaaattca gccgtttgtt gccaccctga tcctgatggt cgccgggcgc ggcgtggcgc
    15181 aactgatcac ctccggacag atcgtcacgt ttaactcgcc ggatctctca tggtttggca
    15241 gcggatcgct attgttcctg ccaacgccgg tcattatcgc ggtgctgacg cttctcctgt
    15301 tctggctgtt gacccgcaaa acggcactgg ggatgtttat cgaagccgtt ggtatcaata
    15361 ttcgggcggc aaaaaatgcc ggggtaaaca cgcgaatcat cgtcatgctc acctacgtgt
    15421 tgagcgggct atgtgcggcg attgcgggca ttatcgtggc ggcggatatt cgcggtgccg
    15481 atgccaataa cgccgggtta tggctggaac tggacgccat tctcgcggta gtgattggcg
    15541 gcggatcgct gatgggcggg cgctttaacc tgctgctttc ggtggtgggg gcactgatta
    15601 ttcagggaat gaacaccgga attttgcttt ctggatttcc gccggagatg aaccaggtcg
    15661 taaaagcggt ggtggtgctt tgcgtgctga ttgttcagtc gcaacgcttt atcagtctga
    15721 ttaaaggggt acgtaaccgt gataaaacgt aatttgccac tgatgatcac catcggcgtc
    15781 tttgtgttgg gttatatcta ctgtctgacc cagtttcccg gttttgcttc cacaagagtg
    15841 atctgcaata tcctgaccga taacgctttt ctggggatca tcgccgttgg catgaccttt
    15901 gtgatcctct ccggtgggat cgatctctcc gtcggttcgg tgatcgcctt tactggcgtg
    15961 tttctggcaa aagtgattgg cgacttcggc ctctcgccgt tgctggcatt tccgctggtg
    16021 ctggtaatgg gctgtgcctt tggcgcattt atggggcttt tgatcgacgc cctgaagatc
    16081 ccggcattta tcattaccct ggcgggaatg ttctttttgc gtggcgtcag ctatctcgtt
    16141 tcggaagagt cgatcccgat aaaccatcct gtttatgaca cgctctcaag ccttgcgtgg
    16201 aaaatccctg gcggcggtcg cttaagtgcg atggggctgc tgatgctggc ggtggtggtt
    16261 atcggcatat tcctcgcgca tcgtacgcgt tttggtaatc aggtgtacgc cattggcggc
    16321 aacgcaacgt cggcgaacct gatggggatt tccactcgca gcaccactat tcgcatttat
    16381 atgctctcta ccggactggc aacgctggcg gggattgtct tctcgattta tacccaggcc
    16441 ggatatgcgc tggcgggcgt aggtgtggaa ctggacgcta tcgcctcagt ggtgattggc
    16501 ggtacgcttt tgagcggtgg cgttggaacg gtattaggga cgctttttgg cgtggcgatt
    16561 cagggactga ttcagactta cataaacttt gatggcacgc tgagctcatg gtggacgaaa
    16621 atcgccatcg gtattttgtt gtttattttt atagcattac agcgtggatt aacggtgctg
    16681 tgggagaatc gtcagagttc gcctgtgaca agagtcaaca ttgcgcagca ataaaaacgc
    16741 ctctccgtgt ggagaggcgc aggagattac gcgtccggga actcacggat aaagcgttcg
    16801 acatcttcaa ccatatggtc gttgccgaca aagaacgaac ggcgctggtg cagggtttcc
    16861 gggatgatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc ttgttccgcc
    16921 aggaatgcca tcgggttgca ctcatacagt aaacgcagtt taccgtccgg gtggctggcg
    16981 gtgcttgggt agagataaat accgcctttc agcaggttac ggtggaaatc cgcgaccagt
    17041 gaaccgatat aacgtgaggt ataagggcgg ttggtggact tatcttcttc ctggcagaat
    17101 ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt gatggagtag
    17161 gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac gccgagcgaa
    17221 ggatcgtaag taaaggcgtg aacaccgcat ccggtggtgt aaaccagcat ggtagaggag
    17281 ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa atcttcttcc
    17341 gttaccggcg tgccgacagg cgtaacgcgg cggtagatgg agaaaatggt accgacagag
    17401 acgttaacat cgatgttgga cgagccatcc agggggtcca tcagcaccac gtattttgcg
    17461 tgttcacagc cttcaaagac gacaatctca tcttcttctt cagaggcaat gcctgcaacg
    17521 atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc gagtttctgc
    17581 tgaacctcgc cctgcacatt ctcagcaccg ctggcaccca ggatatcaac cagtcctgct
    17641 ttgttgatat cgcgatggat aatcttggcg cccagtttta ttgccgacag caaagcagtg
    17701 agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc acctaacgtt
    17761 ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat cttaacaaat
    17821 aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt acggattatc ctgaaatgcg
    17881 tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg atagtgacgt
    17941 atgcgcattc atattttagg aatttgtggc acgtttatgg gcggtctggc gatgctggcg
    18001 cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc gatgagcacc
    18061 ttacttgaga agcaaggcat tgaactgatt cagggttacg atgccagcca gctcgaaccg
    18121 cagccggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg tgtggaagcg
    18181 gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga ttttgtgctg
    18241 cgcgaccgct gggtgctggc cgttgccggt acacacggca aaactaccac cgcgggaatg
    18301 gcgacctgga ttctggaaca gtgcggttac aaaccgggat ttgtgatcgg cggtgtgccg
    18361 gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat cgaagcggat
    18421 gagtatgact gcgccttctt cgacaaacgc tctaaatttg tccattactg cccgcgtacg
    18481 ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct gaaagcgatc
    18541 cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggccgtat tatctggccg
    18601 gaaaacgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga gcaggagctg
    18661 gtgggcgagc agggtcactg gcaggcgaaa aagctgacca ccgatgcttc cgaatgggaa
    18721 gtcttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtagg cgaacataat
    18781 atgcacaatg gcctgatggc gattgcggcg gctcgccatg ttggtgtagc gccggcagat
    18841 gccgctaacg cgctgggttc gtttattaat gctcgtcgcc gtctggagtt gcgtggtgaa
    18901 gcgaatggcg tcacggtata tgacgatttt gcccatcacc cgacggcgat tctggcaacg
    18961 cttgcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt gctggaaccg
    19021 cgctcgaata ccatgaaaat ggggatctgc aaagacgatc tggcaccttc attaggtcgt
    19081 gccgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc agaagtggca
    19141 gaagcctgcg ttcagcctgc acactggagc ggcgatgtcg atgcgctggc agatatggtg
    19201 gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaatggcgg ttttggtggg
    19261 atccatcaga aactgctgga tgggctggcg aagaaagcgg aagccgcgca gtaattctga
    19321 agccttgcat gtgtgtatgc cggataaggc gtttacgccg catccggcat ttgagcatag
    19381 tacctgatgc gacgcttgat gcgtcttatc aggcctacag gtgcaccgca tccggcaaac
    19441 catactcaca tcaacaacga aaattaccct tcgttctccg ctaactcgcg cagatactgg
    19501 aaaatctggc gtgcggattt cggcggctta ttcccttctt tctctttctt cgcgttacgg
    19561 atcagagtac gcagttgctg acgatcggca tcgggccaca gattcaatac ttcagcgatg
    19621 gcgtcatcac cctggtcgat cagacgatcg cgcaggtttt ccagtttatg gaacagcacc
    19681 acctgctgat tatggcgatt tttcagctta tccagcgcct gacggatcgg ctcaacatcg
    19741 cgctggcgca gcatcttacc gatgagctgc aactggcggc ggcgaccttc catcttaata
    19801 cgctgggcga gctcaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg
    19861 tttttcccca gatcaacaat ttccgcgcca aggcgtttta gctcctcggc atcacgttta
    19921 atttcacttt tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga
    19981 acgtcgtcga gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct
    20041 aatgttacca gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc
    20101 tctacattat ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc
    20161 tggaagaagc agtttcgact gcgctggagt tggcctcagg caaatcggac ggtgcagaag
    20221 ttgccgtcag caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg
    20281 ttgaatttaa tagcgatggc gcgctgggga tcactgttta tcaccagaac cgcaaaggta
    20341 gcgcatcgtc taccgattta agcccgcagg ccattgcccg caccgtacag gcggcgctag
    20401 atattgcccg ttatacctcg ccagatccct gtgccggcgt ggcagacaaa gagctgctgg
    20461 cctttgacgc accggatctc gacttgttcc accctgcgga agtttccccg gatgaagcca
    20521 ttgaactggc ggcccgcgca gaacaggcag cattgcaggc ggacaaacgc atcaccaata
    20581 ccgaaggcgg cagctttaac agccactacg gtgtcaaagt ttttggcaac agccacggca
    20641 tgttgcaggg ttactgctca acgcgtcatt cgctctccag ctgtgtaatt gccgaagaaa
    20701 atggcgatat ggagcgtgat tacgcctaca ccattggtcg tgcgatgagc gatctgcaaa
    20761 cgccagagtg ggttggggcc gactgtgctc gccgcacttt atcgcgtctg tcaccgcgta
    20821 aactctccac catgaaagcg ccggtcattt tcgccaatga agtggcgacc ggtctgtttg
    20881 gtcacctggt cggcgcgatt gccggcggtt cggtgtaccg caaatccacc ttcctgctgg
    20941 attctctggg caaacagatc ctgccggact ggctgaccat tgaagagcat ccgcatctgc
    21001 tgaaagggct ggcgtcgacg ccattcgaca gcgaaggtgt gcgcaccgag cgccgcgata
    21061 tcgtcaaaga tggcatcctg actcagtggc tgctgaccag ctattcggcg cggaaactgg
    21121 ggctgaaaag caccggacat gcgggcggta ttcacaactg gcggattgcc ggacaagggc
    21181 taagcttcga acaaatgctt aaagagatgg gcaccgggct ggtggtgacg gagctgatgg
    21241 gccagggcgt gagtgccatt accggtgatt attcccgtgg tgcagcgggc ttctgggtag
    21301 agaatggcga aattcagtat cctgttagtg aaatcaccat cgcaggtaat ttgaaagata
    21361 tgtggcgcaa tattgtcacc gtcggtaacg atattgaaac acgcagtaat atacagtgtg
    21421 gttctgtgct gttgccggag atgaaaatcg ccgggcagta aaaaactggc gcgacctgcc
    21481 gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat
    21541 gagggccggg gagcgtactc gcagtaggtg acccggtcga atgagcgtag ccgtcgatga
    21601 ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgta aaagcctgtt
    21661 agctattctt gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga
    21721 agacaatatg gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc
    21781 gcaagtcaaa gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcgac
    21841 gccgccgaag ctcgaaggca aatcaccgga cagcccggaa atgaaagact tccgccacgg
    21901 tttcgacatt ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt
    21961 aaaagaagcg caggccgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa
    22021 gtatcgttaa ttccttattg ggcggtgaaa cgttaccgcc caacgctttc ttctcccttc
    22081 gtaaaatcac cgcaatatct atcttcacca cggcatattc ctgcgtgtcg tcctcatttt
    22141 tgcgaggcgt tttccagaaa tgaatgtaat gcatatgtaa tgctataatg cattacactt
    22201 ttcataatta ccggaggcat aatggccaca cttaacgtcc gcctggatga caaactgaaa
    22261 aatgaggctt atgcggtgct ggaaaaatta aatatcacgc ctactgaagc agtgcggctg
    22321 ctgtttcagt acgttgcgga aaatggacga atgccggtaa aaacgctcac tgtcagtgat
    22381 agtgaagaag ccttgttacg cactgtcagg gaacgactgg ccaacccaca agaggccatt
    22441 aaggtccgac tggatgaact atgagctggc gtttgatccg cgggcgctga aagagtggca
    22501 aaaactgggc gtgacagtcc gggaacaatt taaaaaaaag cttgaggatg tgctgaaaag
    22561 gccgcgcaat ccatcggcta aactgcggga tttaccggac tgctacaaaa ttaagctgcg
    22621 cacacaggga tatcgcctgg tttatcaggt taacgataaa gagttgctcg tgttggtgat
    22681 agctattggt aagagagaga actcagccgt ttatgaagac gcgactaaac ggctggatga
    22741 gtgactaacg caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt
    22801 cttttaaatc ctgcacaaat ttgccgtcga ccagtacgtt aatcaaatca acaacctgca
    22861 tttgcgcagc gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg
    22921 gacattcggc gcggatgcgt tgtaccagtt tcagaatatc cggcacgttt tgcggatgca
    22981 gcggatcgcc gccggagagg gaaatcccct ggcgtttgat gcgagtgtca ttcagatcgt
    23041 taatgatctg gtcttccatt gctttggtaa atggctgacc ggaatttacc cgccatgtgc
    23101 ttttgttata gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag
    23161 tgccggggcc gttgacgatg tcgacaggat agtactgatg aaagttcatt ttcgcttctc
    23221 aattgacgcc atgactgctc tggtgcgtac gggcgaagtc ccccgatgct acggccacct
    23281 tgcgacgaca ccaactggcc agaggcaatt cattatgtcg ggaatcagga aggggcacgg
    23341 aacggtaccc tctcccttga gggagagggt tagggtgagg gggaaggatt aacctatctg
    23401 cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccgg cgttaaatgg
    23461 acgtgcatcc gggctaccta aatatccgca cacgcggcga gtcaccgaca cacgggaggc
    23521 gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcaa actcgccggt
    23581 aaagccacac tcgtagcatt catcgatcgg tgtgttggtg ccgtaatacg gtacatgctg
    23641 atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt ttggatactc
    23701 gccatagcaa atgaagccac cgttcgccag tggcgggtaa ggcgcttcaa agtcgatctt
    23761 gtcgtacggg ttcaccttct tctccacatc gaggtggaaa ctgttggtgt agtaaccttt
    23821 atcggtcacg cccggcacca cgccaaactc agcggtatcg agacggcaga agcggtcgca
    23881 caggttttca ctcggcgtgc tgtagagact gaagccataa cccgtttctt ctttccactg
    23941 atccactgcc tgacgcagac gttcaacaat cgcaatacct ttcgcgcgca gctgctcgtt
    24001 gtcgtaaaca tgctcgccgc cgaacagcgc gttaatggtt tcgtggatgc cgatgtaacc
    24061 cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acgtcgtcgt cagcattcag
    24121 acgcacgcca caagcgcctt ccatatagag gatcggggct acgcgcgctt tcacgccttc
    24181 gagacgagcg atacgggtca tcagcgcctt acgtgccagc accagacgtt catccagcag
    24241 cttccagaag gtggcttcat cgccttttgc ctccagagca atacgcggca ggttcaggct
    24301 gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat tttcccacac
    24361 gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgactt tcactacctg
    24421 atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg ccagctgttt
    24481 gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg caaacaccag
    24541 tttcgggaac accgccgttt tacggttttt accgaggcct gcgatacggt tacgcaggat
    24601 agattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa aggtcacaaa
    24661 cggcgtttga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg actggaaggc
    24721 atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg ggatgttcca
    24781 ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg ccagcacttc
    24841 atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctggg caataatctg
    24901 cgcagttaca gcggttgccg tagagatcga cttcggcggt tcaatctcgg cgttccccat
    24961 tttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga acatcgggaa
    25021 gaacggtgag taatcgagat cgtgatagtg aatatcacca cgttcatgtg cctgcaccac
    25081 gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg ccagcaggtc
    25141 gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta acgaggagtt
    25201 ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt cacgttcaat
    25261 gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg gacccgacat
    25321 cagctggttt tcaactgcgg tctggatctc attgatatcc acctggttgc ggccctgcat
    25381 ctgctcgctg acaaccgcag caacagtggc gcaataatcg gcatcatcga cttccgctgc
    25441 tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca ctttgcagcc
    25501 gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac aggttatcca
    25561 cagaaattgg gaaagacgtt ttccggtttt tttgtttcgt tttccggtgc tttccgcaat
    25621 cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt agcaattata
    25681 gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat agtgctttgc
    25741 atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag cgtacggatg
    25801 acgggcgcta cagcgatatg taaatttttt aatgaatttt ttgattataa aatcaacaaa
    25861 aacaacatac tgacaggcaa aaccccggaa tgacaggcat taccggggct tagcgaagat
    25921 ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggcac agggttgtgg
    25981 tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgagcattt tccctggctg
    26041 ccagggttgg gtctcacggc taaggttggc aatgaccaac aaggtttgcc ccttccattc
    26101 acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat cgccccatgt
    26161 caggacggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa acaccgagga
    26221 ctcatcggtc agcgcagctt ctacgttgat ttcctgatag ttatcgccca gaccaatcca
    26281 cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg gtgtgcgact
    26341 gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac gaccatcgtt
    26401 gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa tgcgcgtgaa
    26461 atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc cctgcatgcc
    26521 atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt catcaccaaa
    26581 gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc atgccacgtt
    26641 gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt caggtttagc
    26701 cagcgtccat ttctcaccac cgggataatc gaccttcagg tgatgaaaat taaaggtcat
    26761 cgacaattca ctgcctgtca gagccgcgta tcgctggcaa tgctcaaggc tggtggagga
    26821 catttcacct acggtcatta acacgcgtgg cgtaaacaca tcgcggttca tctcgtgcaa
    26881 aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat ccaggtcttc
    26941 agggtaacgc ggatctttgg agatcagatt caccacatcc agccgcaacc cgtcgacccc
    27001 acgatcggcc cagaactcac agactttttt cagctctgcg cgtaccgctg gattctccca
    27061 gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc tttccgcatg
    27121 ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg tttctggttc
    27181 gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg cctcgcgaaa
    27241 ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac gaatcccgcg
    27301 cgattttgcc tgtgtcacca gttcgtcaaa atcgtccagc gtgccgtagg tgggatcaat
    27361 cgccgtatag ttcgctacgt cgtaaccgtt atcaacctgg ggagagacat aaaagggggt
    27421 tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgctgga taacgccacg
    27481 taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa tctggtagat
    27541 aacgccgttt tgccaccagt ggggaagatg agtcattacg ttattcctgc aaatgcgagg
    27601 gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg cggtatttcc
    27661 gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc attgccagcg
    27721 caaacacctg ccagtagctc ggttgaatcg agagaattcc cggcaggccg cctacgccga
    27781 tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca gaaccaatca
    27841 tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacatagca ggctcagtga
    27901 ctccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg tgcttgcggc
    27961 tggaaatgat aatgcctatc acggcggaac cctgagcgat attcgacagc gcaatcagcg
    28021 gccacactgg cgtaccaccc atgctttgaa tcatctgcaa atcaatagca agcgtggtct
    28081 gatgtacgcc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc gcgccaatcg
    28141 gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg ccaatcatgc
    28201 gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc gaacagacgg
    28261 gcaccaccac cagatagagg taatccggca cgatgcgctt aagacgagtt tcaataacgc
    28321 ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc actttggcga
    28381 tgctgaacat gccaaagtcc catacttccg gcagctgctg cccgagcaga taagcgttca
    28441 tcagctgtgg agaaaccagc gttacgccaa gcacgatacc gaggatcggt gtaccgccca
    28501 tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc gcttcaccga
    28561 tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc agcgtctgac
    28621 cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa ccaccactaa
    28681 tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac tgctcatgcc
    28741 atttcatatt ctgccgtgcg gcttttttta cctgctcttt atcaacctgc gcctgtccgg
    28801 ttgacgctat cagtgcttga tagtaatcac ccacgttggt gccaattacc acctgaaatt
    28861 gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct ttcggtctgg
    28921 cattggccgg ttggttgagg acaaagcgca gacgagtaat acagtggctc accgtcgcaa
    28981 tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg tttattttgc
    29041 tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat attgcgcgga
    29101 aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta tcgttcagga
    29161 aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg cccggttacc
    29221 tgcgcgatca actggcaggc cgcctggcgt ccagcttcgg cgtaaccggg atctacggtg
    29281 acgatctccg gatggaggaa tttcattaac ggcgtattac cgacgctcgc cagttgcaag
    29341 gtctcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt gtcggttgcg
    29401 cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttctcata gccttgcttc
    29461 atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcactttgca gaacgccagg
    29521 taggcttcgt gacgtcgctt accggtcgtc acgtcactat gcggcacgcc gagataactg
    29581 atattacgat gcccctggtc atacagccgt tgcatcagga tcttgattgc cccttcgtcg
    29641 tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag cgatgactgc
    29701 cagtgggcta acatttcttc tgttatgccg gtaaaaccga acagcactac gccgtcgata
    29761 ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gcggggaaaa ctgactttcc
    29821 atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg aacggcgaga
    29881 ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc gctttgccca
    29941 cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac tgcttcaaca
    30001 cgctcgcgcg tgcgctggct cacgccgctt tcgttattca gcacccggga aaccgtagat
    30061 ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg catcctgttg
    30121 tcccgtaacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc agcaatgggt
    30181 aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat agatgctacg
    30241 aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg aggttatatg
    30301 gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta agcccgtctt
    30361 ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct cagctttatt
    30421 accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca gacagtgcgg
    30481 agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta ccttcccgtc
    30541 tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg gtcccgccgt
    30601 ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg tggaaaacgt
    30661 tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc gaacaacatg
    30721 gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggttcattta tgggtctgct
    30781 atcgcaaccc ctttaatatt ttactcacca ttctcggcgc tatttcttac gccacggaag
    30841 atttatttgc cgcgggtgtt atcgcgctaa tggtcgctat ttctacgttg ctgaacttta
    30901 ttcaggaagc acgttccact aaagcggcag atgccctgaa agcgatggtc agcaataccg
    30961 cgacggtgct gcgcgtaatt aacgacaaag gcgaaaatgg ctggctggag atccagatcg
    31021 accagctggt gcccggcgat attataaaac tggcggcagg ggatatgatc ccggcagatt
    31081 tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc ggtgagtctc
    31141 tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatcca ctggagtgcg
    31201 acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca atggtgattg
    31261 ctacaggagc caacacctgg tttggtcaac tgacggggcg tgttagtgag caggaaagcg
    31321 agccgaatgc ctttcagcaa gggatcagcc gcgtcagtat gctgctgatt cgctttatgc
    31381 tggtgatggc accggtggtg ctgttaatca atggttacac caaaggcgac tggtgggaag
    31441 cggcgctgtt tgcgctttcg gtcgcggtag gcttaacgcc agaaatgttg ccgatgattg
    31501 ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg atcgtcaagc
    31561 atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat aaaaccggca
    31621 ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt aaaaccagcg
    31681 aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccgggctt aaaaacctgc
    31741 ttgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg gccagccgtt
    31801 ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg ccggatgtcg gtggtagtgg
    31861 cagaaaatac cgaacaccat cagctggttt gcaaaggcgc attgcaggaa atcctcaatg
    31921 tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccact cgatgacacc atgctgcgta
    31981 agattaagcg ggttaccgat acgctgaatc gtcaggggct gcgcgtggtt gcggtggcga
    32041 cgaaatacct gccagcgcgt gaaggcgatt accagcgagc ggatgaatcc gacctgatcc
    32101 tcgaaggata tattgctttc cttgatccgc caaaagagac aactgctccg gcactgaagg
    32161 cattaaaagc gagtggcatt accgtaaaaa tccttactgg cgacagtgag ttagttgcag
    32221 cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt agtgatattg
    32281 aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg tttgctcgcc
    32341 tgacgccgat gcataaagaa cgcattgtga ccttactgaa gcgcgaaggg catgtggttg
    32401 gctttatggg cgatggtatt aatgatgcgc ccgccttacg tgctgcggat atcggcattt
    32461 ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg ctggaaaaaa
    32521 gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc aacatgctga
    32581 aatacatcaa aatgacggcg agctctaact tcggtaatgt gttcagtgtg ctggtggcga
    32641 gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag aacctgctgt
    32701 acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa attcaaaagc
    32761 cgcagcgttg gaatccggcg gatttggggc gctttatgat cttcttcgga ccgatcagct
    32821 cgatcttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc aacacgccgg
    32881 aaacgcaaac gctgttccag tcgggctggt ttgtggtggg cttactgtcg caaacgctga
    32941 ttgtgcatat gatccgcacc cgtcgtgtac cgtttattca gagctgtgca tcgtggccgt
    33001 taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt tcaccgctgg
    33061 ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg gttgcgattc
    33121 tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt cgttacggct
    33181 ggcagtaaag aataaactgg gcatggtggc ccgaatccat attgcagaca aagtgagctt
    33241 tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat aaattgcagg
    33301 aatcacgtag gcctaataag cgtagcgcat caggcgattt tgcgtttgtc atcagactta
    33361 atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga tcgcttcgat
    33421 ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg ccgggaaggt
    33481 ggcgttgtgt tcggtgaaga aggcttcgta ggtagcatta acggttgcga agtcgttcag
    33541 atcttttaca aacacggtag ttttaacgat gtcgcccact ttcaggccag cggcttcgac
    33601 gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacgtctg ccggtacttc
    33661 gcccgttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc ccagatcaac
    33721 gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag ttttgctcat
    33781 gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc agacttttat
    33841 taccaaccgc aattaattgg ccagcaccac attatgagaa aactcttttt cacagtattt
    33901 gcatttgagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg aaaccggttc
    33961 ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc gctccggcag
    34021 acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg tggcttgcgg
    34081 tgcatacaat gccagttggt ctacttgatc ttcactcaaa aaggtatttt cgattttgat
    34141 cagatctttg cggcccatct cgccagaagg caggttcaga ccaatggtga tgcgctgatc
    34201 cgtttcggtc agcttgaaca aactcaacag cttaaaaccg atctgggcgg ggatatggtc
    34261 aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg tcatctctat
    34321 ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct ggcgagcgaa
    34381 aatcccgttg cctgcctgct ggaagtacca ggcgtgcggc gttttatcaa catcggtggc
    34441 aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg cgttgtggag
    34501 atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg ggtccagacg
    34561 ctctttttgc acgcgagtca tgtacaggat gtctacttcc gccatcactt cttcaataga
    34621 gctgtgcaga ctccaggcga tccctttttc atcgagcata tcaaggatgt attgcggcat
    34681 tgccaacgcg tccggcgcga tgaagtaaaa acggttgccg tcgaacttcg ctaacgcctg
    34741 agtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga cgtggagatt
    34801 gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt gcgtcggatg
    34861 ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaatt cggtggccag
    34921 gcgcgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag tgctgataac
    34981 cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg cgctgtcgga
    35041 gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga ggcgggtacg
    35101 ggtagaagct tcgaagaaac agctggcaat gactttgtgc ttcaacagct ctggttgcgg
    35161 gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc gactaaggtc
    35221 gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc tcctgacgcc
    35281 tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg ggaagaaaaa
    35341 cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac actgaaccat
    35401 acatcctccc ggcaaattgt ccggcattat actcatcgtc agaagcggtg caagcatttg
    35461 atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat acatgcattt
    35521 ttatttgata tgattctggg ggagaactgt cgttcggtat caataattgc agacatttcc
    35581 tgcttttttc tttttcactt tcacgcaatc agatatgcat tttattccat tcatccgact
    35641 tatagggcgg agtgtgaaaa cgaacggcta acactattgc ttactgcgca gggatgcgcg
    35701 ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc gccgtccagt
    35761 ttggataagg tggggcgcta aatatttcat ttttgaccgt catgatgtct tcaaattgtt
    35821 tttctggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg catcccgcag
    35881 cagccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga aaatcgggtt
    35941 ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa tcgccggaac
    36001 gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca gggaaaacag
    36061 cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa gatttatatt
    36121 taccgggagc cgcttttagc ggacgacgta agtaaacaaa gcccagacat catggataat
    36181 ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat ggtatgcatc
    36241 gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc agcgacctct
    36301 tccggttgcc catgtctttt gatggccata aaaccatgca acatatcgcg cattggcccg
    36361 ttggcgggat tagcgtcggt atctattggc cctggctgga cgacgttgat ggttatccca
    36421 cgcggtccaa aatcacgggc cagcccgcgt gccatgcctt gcagggcaga tttactggcg
    36481 gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc gatgattaag
    36541 atacgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata aggagcatga
    36601 atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc atcgccaaag
    36661 acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg aacgacatca
    36721 ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt ctcatgtgcc
    36781 aggcgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc tccatcggtg
    36841 acgaaacgac gtacgatagc ggcaccgatt ccacggctac caccgaggat gagaactgtc
    36901 ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc attacaaaac
    36961 tgttttaact ttctgtcaac aagttttgta atggtcacta aaaaacaatc tcgcgttcca
    37021 ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt gctttttcac
    37081 caaaaaggtt tcgatgctgt cagtgttgct gaagttactg attatcttgg tattaacccc
    37141 ccgagcctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt actcaatgaa
    37201 tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg tccggtaggc
    37261 gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa cggcggctgc
    37321 gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg tgatattgcc
    37381 gttcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag gcggcatcca
    37441 caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct ttctgcgaag
    37501 gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc gggggaagcg
    37561 ataaaaactc ttatcaagga gtgatgctgg ccttgatccg aaaggcggga acgcgcctgc
    37621 cgataagttg tgataagaca ataattcacg cgttaaggct agcggaattg atcatctttt
    37681 cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat gatcatcgga
    37741 aatattcata atcttcagtc gtggctaccc caggagttac gccaggcgat tgagcatatc
    37801 aaagcacacg ttacggcaga aacgcccaag ggcaagcacg atatcgaagg caatcgtctg
    37861 ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc ggagtaccat
    37921 gcccgctatc tcgacattca gattgtgtta aaaggtcagg aaggcatgac cttcagcacg
    37981 caacctgcag gcaccccgga taccgactgg ttagcagaca aagacatcgc atttttgccg
    38041 gaaggcgttg atgagaaaac agttatcctt aatgaaggtg attttgttgt gttttatccg
    38101 ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg caaagcagta
    38161 gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt tacggcgagt
    38221 ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg cttagacgtt
    38281 cttattaact taaggagttc gtcatgagca aaatatcagg ttggaatttt tctcaacaca
    38341 ttacatcagc cgacaatttt aaacaaaaaa gtgaagttat aaatacctgg cgtgttggaa
    38401 tgaataattt tgcccgtaat gccgaggggc aggataatac aagaaatatc cttgatccta
    38461 agacattttt ggagtttttg gtaaaaatat ttaccctggg ttatgtggat tttagcaaac
    38521 gctccaatga agcgggaaga aatatgatgg ctcatattga gtcctcatct tatatcaaaa
    38581 ataatgatgg cagtgagata atgaagtttg ttatgaataa tcctgaaggg gaacgagcag
    38641 atttatctaa ggtggaaata gaaattacac tttccactat cactactatg gggactcgtc
    38701 aaggacatac ggctattata tttcaacaat ctgatggttc gactaatcgt tatgaaggga
    38761 agtcctttga aagaaaagat gagagttcat tacacctgat tactaacaag attctggcgt
    38821 gttaccaacg cgaagctaac aaggaaatag ctcgtctatt aaatattcc
//
LOCUS       DARNVM010000022        38230 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.022, whole genome shotgun sequence.
ACCESSION   DARNVM010000022 DARNVM010000000
VERSION     DARNVM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.022"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(78..269)
                     /locus_tag="SI621_001109"
     CDS             complement(78..269)
                     /locus_tag="SI621_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HEI4063850.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            287..439
                     /locus_tag="SI621_001110"
     CDS             287..439
                     /locus_tag="SI621_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063851.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            621..2864
                     /gene="pdeF"
                     /locus_tag="SI621_001111"
     CDS             621..2864
                     /gene="pdeF"
                     /locus_tag="SI621_001111"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HEI4063852.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2903..4444)
                     /gene="ppx"
                     /locus_tag="SI621_001112"
     CDS             complement(2903..4444)
                     /gene="ppx"
                     /locus_tag="SI621_001112"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEI4063853.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4449..6515)
                     /gene="ppk1"
                     /locus_tag="SI621_001113"
     CDS             complement(4449..6515)
                     /gene="ppk1"
                     /locus_tag="SI621_001113"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEI4063854.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6686..7324)
                     /gene="purN"
                     /locus_tag="SI621_001114"
     CDS             complement(6686..7324)
                     /gene="purN"
                     /locus_tag="SI621_001114"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEI4063855.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAIVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7324..8361)
                     /gene="purM"
                     /locus_tag="SI621_001115"
     CDS             complement(7324..8361)
                     /gene="purM"
                     /locus_tag="SI621_001115"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEI4063856.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8686..9312
                     /gene="upp"
                     /locus_tag="SI621_001116"
     CDS             8686..9312
                     /gene="upp"
                     /locus_tag="SI621_001116"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEI4063857.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9398..10687
                     /gene="uraA"
                     /locus_tag="SI621_001117"
     CDS             9398..10687
                     /gene="uraA"
                     /locus_tag="SI621_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEI4063858.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10737..11483
                     /gene="hda"
                     /locus_tag="SI621_001118"
     CDS             10737..11483
                     /gene="hda"
                     /locus_tag="SI621_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HEI4063859.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11589..11948)
                     /gene="arsC"
                     /locus_tag="SI621_001119"
     CDS             complement(11589..11948)
                     /gene="arsC"
                     /locus_tag="SI621_001119"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEI4063860.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11969..13432)
                     /gene="bepA"
                     /locus_tag="SI621_001120"
     CDS             complement(11969..13432)
                     /gene="bepA"
                     /locus_tag="SI621_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HEI4063861.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13645..14706
                     /locus_tag="SI621_001121"
     CDS             13645..14706
                     /locus_tag="SI621_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEI4063862.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14744..15592)
                     /locus_tag="SI621_001122"
     CDS             complement(14744..15592)
                     /locus_tag="SI621_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HEI4063863.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNVMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15614..17626)
                     /gene="hyfR"
                     /locus_tag="SI621_001123"
     CDS             complement(15614..17626)
                     /gene="hyfR"
                     /locus_tag="SI621_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HEI4063864.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLAHIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSVSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17656..18069)
                     /gene="hyfJ"
                     /locus_tag="SI621_001124"
     CDS             complement(17656..18069)
                     /gene="hyfJ"
                     /locus_tag="SI621_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HEI4063865.1"
                     /translation="MAEECREIVFWMLRKKFIASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18062..18820)
                     /gene="hyfI"
                     /locus_tag="SI621_001125"
     CDS             complement(18062..18820)
                     /gene="hyfI"
                     /locus_tag="SI621_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HEI4063866.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18817..19362)
                     /gene="hyfH"
                     /locus_tag="SI621_001126"
     CDS             complement(18817..19362)
                     /gene="hyfH"
                     /locus_tag="SI621_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HEI4063867.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDYQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDEPLVAKEQL"
     gene            complement(19372..21087)
                     /gene="hyfG"
                     /locus_tag="SI621_001127"
     CDS             complement(19372..21087)
                     /gene="hyfG"
                     /locus_tag="SI621_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HEI4063868.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKIVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21077..22657)
                     /gene="hyfF"
                     /locus_tag="SI621_001128"
     CDS             complement(21077..22657)
                     /gene="hyfF"
                     /locus_tag="SI621_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEI4063869.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHGLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLLIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22662..23312)
                     /gene="hyfE"
                     /locus_tag="SI621_001129"
     CDS             complement(22662..23312)
                     /gene="hyfE"
                     /locus_tag="SI621_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HEI4063870.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFVAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23324..24763)
                     /gene="hyfD"
                     /locus_tag="SI621_001130"
     CDS             complement(23324..24763)
                     /gene="hyfD"
                     /locus_tag="SI621_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HEI4063871.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGGVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24780..25727)
                     /gene="hyfC"
                     /locus_tag="SI621_001131"
     CDS             complement(24780..25727)
                     /gene="hyfC"
                     /locus_tag="SI621_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HEI4063872.1"
                     /translation="MRQTLCDGYLVIFALAQAVTLLVLTPLFTGISRQIRARMHSRRG
                     PGFWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFHFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISKTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKLGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25738..27756)
                     /gene="hyfB"
                     /locus_tag="SI621_001132"
     CDS             complement(25738..27756)
                     /gene="hyfB"
                     /locus_tag="SI621_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEI4063873.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVMVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAIDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVTSGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWEHAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27756..28373)
                     /gene="hyfA"
                     /locus_tag="SI621_001133"
     CDS             complement(27756..28373)
                     /gene="hyfA"
                     /locus_tag="SI621_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HEI4063874.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28626..29096)
                     /gene="bcp"
                     /locus_tag="SI621_001134"
     CDS             complement(28626..29096)
                     /gene="bcp"
                     /locus_tag="SI621_001134"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEI4063875.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29096..29668)
                     /locus_tag="SI621_001135"
     CDS             complement(29096..29668)
                     /locus_tag="SI621_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HEI4063876.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29814..30692
                     /gene="dapA"
                     /locus_tag="SI621_001136"
     CDS             29814..30692
                     /gene="dapA"
                     /locus_tag="SI621_001136"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEI4063877.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30709..31743
                     /gene="bamC"
                     /locus_tag="SI621_001137"
     CDS             30709..31743
                     /gene="bamC"
                     /locus_tag="SI621_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEI4063878.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31956..32669
                     /gene="purC"
                     /locus_tag="SI621_001138"
     CDS             31956..32669
                     /gene="purC"
                     /locus_tag="SI621_001138"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEI4063879.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32837..33700
                     /locus_tag="SI621_001139"
     CDS             32837..33700
                     /locus_tag="SI621_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEI4063880.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33715..35730
                     /gene="tmcA"
                     /locus_tag="SI621_001140"
     CDS             33715..35730
                     /gene="tmcA"
                     /locus_tag="SI621_001140"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HEI4063881.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPVAENHCFPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     VHFAPRTDWHPATGTPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVIAAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIVGALQYTHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35804..36502
                     /gene="ypfH"
                     /locus_tag="SI621_001141"
     CDS             35804..36502
                     /gene="ypfH"
                     /locus_tag="SI621_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEI4063882.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGNDDVIEMM"
     gene            complement(36583..36783)
                     /locus_tag="SI621_001142"
     CDS             complement(36583..36783)
                     /locus_tag="SI621_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HEI4063883.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36811..37938)
                     /gene="dapE"
                     /locus_tag="SI621_001143"
     CDS             complement(36811..37938)
                     /gene="dapE"
                     /locus_tag="SI621_001143"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEI4063884.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVEWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37942..>38230)
                     /locus_tag="SI621_001144"
     CDS             complement(37942..>38230)
                     /locus_tag="SI621_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEI4063885.1"
                     /translation="NIDYRFHDYRVDGLDSELLNGFINELGWEALLNTRGTTWRKLDE
                     TTRNKIIDAASAATLMTEMPAIIKRPLLCTPGKPMLLGFSDSSYQQFFHEV"
BASE COUNT         9297 a         9889 c        10078 g         8966 t
ORIGIN      
        1 gctttgataa tcccaaagag caaaagcatc cgacagcgag gtgccgttat gttctgtgaa
       61 agtggagaag gtaaccgcta acgttgtacc ggtgattcga cggagagtgt ggattgctga
      121 gcttcttttt tgctctgatg gtgctgccat gcaccgacgg acgagtagat aaaacggcca
      181 aagaagaaga taaaactgat gagcagtacg atacgggtca tgcgactgtt aaatcggtgt
      241 cgttttcgca tactggttgc ctgactcaca aaaggttcct tgaagtatgt cccacgctgt
      301 ggacggtact tacattaagg cacaacagga caaaatggtc aattcttcgt tatgtaaaaa
      361 agcgtcagtg gatacatatt ttaatgttat ggaagttaat ttaattattt acaatgatga
      421 tgtaataatg atgaattaac atgataaacg taataattca ttaatcaggg ttattttgtt
      481 tgatatatat caattggaac tttcatatga cacttagaat caatgctctc tatagtgatg
      541 aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct tcaggatgtg
      601 ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata cgtgataaat
      661 ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc cacattaata
      721 ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctctg gctctgatga
      781 tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcgcctta gggatttttg
      841 tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct aattttatta
      901 tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt aacaacgttt
      961 cacatggtga tacccgttta atttcccagc gtatattctg gcagattgtg tttcctgcaa
     1021 cgctgtttct gatacttttc cagtttgctg cgtttgtagg attactggcg agcagagaaa
     1081 atctggtcgg cgtcatgcct tttaacctcg ggaccttaat caattatcag gccttactgg
     1141 tgggcaatct gattggtgtc ccgctgtgct acttcatcat tcgtgtagtg cgaaatccgt
     1201 tttatttacg tagttattat tcgcaattaa aacagcaggt tgatgtcaaa gtcaccaaaa
     1261 aagagttcgc actctggcta ctggcattag gtgcgttgtt gttgctgtta tgcatgccgt
     1321 taaatgaaaa aagcacaatt tttagcacca attatacctt gtcattattg ctgcccctga
     1381 tgatgtgggg agccatgcgc tatggttata agctgatttc gctgctctgg gcggtcgtgt
     1441 tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc tataccacgc
     1501 agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat tatatggctg
     1561 tactggcaac ccgtcagaga gcggtagtca gacgcattca gcggcttgcg tatgtggacc
     1621 cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat gccccctggt
     1681 ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaaaaac tatggcatca
     1741 tgctacggat tcaatacaag caaaaacttt ctcactggct gtcaccattg ctggaaccgg
     1801 gtgaagatgt ttatcagcta tcgggtaacg atctcgcgct gcgactgaat acagaatcgc
     1861 accaggagcg cattaccgca ctggatagcc atctcaagca atttcgtttc ttttgggatg
     1921 gaatgccgat gcaaccgcag attggcgtca gttactgcta tgtgcgctcg ccagtgaatc
     1981 atatctactt actgctggga gagctaaata cggtcgccga actttccatc gtgaccaacg
     2041 ccccagaaaa tatgcagcgt cgcggggcaa tgtatttgca acgcgaattg aaagataaag
     2101 tcgcgatgat gaatcggcta cagcaggcgc tggaacacaa ccattttttc ctgatggccc
     2161 agccgattac cggtatgcgt ggcgatgtct accatgaaat tcttctgcgt atgaaaggtg
     2221 agaatgatga actgatcagc cccgacagct tcttgccggt cgcgcacgaa tttggtttat
     2281 cgtcgagtat cgacatgtgg gtcattgagc atacgctgca atttatggct gaaaacagag
     2341 cgaagatgcc cgctcaccgt tttgcgatta atctgtcgcc aacctcggta tgtcaggctc
     2401 gttttcccgt tgaagtcagt cagctgctgg ctaaatatca aattgaagcg tggcaactta
     2461 tttttgaagt caccgaaagt aatgctctga caaatgttaa gcaggcgcaa atcaccttgc
     2521 agcatcttca ggaattaggc tgccagattg cgattgatga tttcggcacc ggctatgcca
     2581 gctatgcgcg gcttaaaaat gtgaatgccg atttgcttaa aattgacggc agttttatcc
     2641 gcaatattgt gtcaaatagt ctggattatc agatagtggc gtcgatttgc cacctggcgc
     2701 gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa cgaagagatc cgcgaggcgg
     2761 tgctctcttt ggggatcgat tatatgcagg gttatcttat tggtaagccg caaccgttaa
     2821 ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc ctgaataatg cgggccgaca
     2881 tttctcgtcg gcccgcaaag cattaagcgg cgatttctgg ggtactttct tcttcaattt
     2941 tcaaccgcca gccagccacg ccttcccagt attcttgctc cttttccaga tcaagcagta
     3001 ccagcgcatt ctgactaaac cagtcatgcg ggaaacgcag tgtccagtga ctgtcatcgg
     3061 taatcagcgt caatgttggc ggtgtagtgg ttgcctgacg ttgattattg agtaatacgc
     3121 caaggcgcaa tagctgaatc agtggcagga actgtttctt cttaaacaag gtaaagcgcg
     3181 gcagatcgtc gagcttaatc gctttacggt gatagcgcac cagcgtcgcc atcatcaact
     3241 gctgttcctg attaaaaccc ggcaagtcgc tgttttgcag aatataagct gagtggcgat
     3301 gcaaaccgct gtggttgata ttcaacccga cctcatgcag catggcggcc catcgcagta
     3361 gcgcctccag ttgcggatgc gccagcttcg gttgctgttc ccgccactgt tcgtacattt
     3421 gcatagtggt atccagtacc cgtcgggcct gttcgctgtc gatgtgatac tggttggcga
     3481 ggctgctggc ggtgcgacta cgcacatcct gatgacggaa acgtccttcc atttcataca
     3541 gtacgccttc gcgtaacgcc ccgtcagaaa ggcgcagttc acggatggct aaagcatcaa
     3601 acacaccgca taaaatcgcc agtcccggaa cgaagactgt tttccgctct tcggaaagac
     3661 ccggtaaact cagcgatgcg aaattacggt gacgtaaaac ttcttttacc agtttttcca
     3721 gacgttccgg ggtaattatc ccgtctttct cgcccatttc cattaacact tcatgggcgg
     3781 cttttatggt accggaagcg cccattgcca cgttccagcc ctgaatacgg aattgccagg
     3841 ttaaagtttc cagtttttgt gctgccgcca tgcgagcgcg ctgaaaattc tctttattga
     3901 tgaccccgcc agggaagtaa agctgggcaa agctgacaca acccatccgg cggctttcaa
     3961 cgagaatcgg ttcgaaattt tcaccaatca ccagttccgt agatccgccg ccaatatcaa
     4021 taaccagttt gcgacctttc tccggttggg tatgttccac gcccataaaa atcagacggg
     4081 cttcttcatt accggaaata atttcaatcg ggtagggaat gaccttttcc gcgcgtttca
     4141 gaaagtcagt ggcgttcagc gcctgacgca gtgtatgggt gccaactata cagacgctgg
     4201 caggagaaaa cccttgcagc cgttcggcaa acagcgacag acagttcaaa ccgcgcgtca
     4261 ttgcctcttc actcaacata ttatctggcc ccaggccgtc cgccagatgc acccgctgtt
     4321 tcaggcggcc aataatctgc atggcaccat ctaccacacg ggctatgacc atgtgaaaac
     4381 tgtttgaacc aagatcgacc gcagcaaact cctgcggacg aggggattta tcgtgtattg
     4441 gcatagggtt attcaggttg ttcgagtgat ttgatgtagt cgtaaatcgc caactgcgcc
     4501 cgtactttgc ggcgattgcc gcggggaacg tagcgattac tgagttcttt atcgatataa
     4561 cgtgctttga ctgtatcgct gaacaatatg tcgatgatgt ccagcacccg ctgcttcagg
     4621 cgcggatcga gcagtggcgt cgccacttca atacgataat caatattgcg cgtcatccag
     4681 tcggcggaag aaaggtagac ctttttatcg ccgccatttt caaaaatata aacccggtca
     4741 tgttcaaggt aacggtcaac aatactgatg gcacgaatgt tgtcgctaat gccttccaga
     4801 ttggggatca gcgaacacat gccgcgaacc agcagattaa ccggtacgcc ggagctggag
     4861 gccgcataca gacgatcaac caggccttta tcgacaaggt tatttagctt cagggtgata
     4921 ccactgggca gcccttgctg cgcgttggcg atctcgcggt ccaccatttc atacaacagg
     4981 cggcgggagt tttgcggcga caccattaaa taatcaaatg tcaccggacg gtatgggttt
     5041 tcaataaagt tgaacacccg ccgtacttcg ttggtgatgc gcgcatcggc ggtcagcaac
     5101 gaatagtcag tataaagacg cgcggttttt tcgttaaagt tcccggtccc gatgtgtgcg
     5161 taacgcacca cttcaccgtt ttctttacgt gaaatcagga acagtttggc gtgaattttc
     5221 agccccggcg cagagaagat aacgtgcacg cctgcttcgg tcaggcgctt cgcccagtga
     5281 atgttggctt cttcgtcgaa acgcgcctgt aactcaacca ccacggtgac tttcttaccg
     5341 ttgtgtgcgg cgtggatcat cgagtcgatg atgcgtgaat ctttcgccac gcggtaaatg
     5401 ttaattttaa tcgccagtac gctcgggtcg aacgaagcct gacgcagcag ttccagcaca
     5461 tgctcaaagg tatgataagg ataatagagc aacacatcgc gttcgcgaat ggcatcaaaa
     5521 ccattgcgga actgggcttt atcaaaccaa atgtggcgta aacgcggcag tggtttgttc
     5581 accagattgg ctttgccgac attggggaaa ttaataaagt ctttaaaatt atgataacga
     5641 ccgccgggga cgatggagtc gtagcgggaa atagtcagtt tttcgcgtaa cacttcaacc
     5701 agcgcattgg gcatatcgcg ctgataaaca aaacgcaccg gctcagcagt taaacgctgc
     5761 ttgagactgg aagacatcaa ctccatcagg ctggcttcca tctcatgcac taaatcgtat
     5821 tcggcatcgc gggtcatctt cattgaatag gcattcagtg cgtcataatc aaagaagcct
     5881 ttgaaaatat catcaaggca gtaacgcaga atgttatcca gaagaatcat cggcttgcgt
     5941 cgacgcggcg cttctggcgg taaattcaca aagcgcggca ctttatctga tgggatctcc
     6001 agcagcgcgt aacggatggt atcgccacgg ataatttcca ccgccagata ggtgtaatca
     6061 tctttcagga actgcactaa gtcagtgtca ggattgatta aaatcggcgt aatgtgctga
     6121 cgcagatact gcttaaaata atgacgcagc cagttttgtt gattgacgga gagctggcgt
     6181 tcattaatca ggaagatctg gttgcgcgcc atctccagca gtaactcgtt gtagaggccg
     6241 tcgaattcct gatcggcttt cagcacccgg gactgaattt tgcccagtaa atggcgggaa
     6301 tgagagttgg agccttgttc ttcgctaata atgatgcgtc gcttcagttc agcgaagcgg
     6361 actttataga actcatcaag gttattggaa tagatcccca ggaaacgcat cctttcaatc
     6421 agcgggttag atttgtccgc cgcttcctga agcacgcgtt cattgaacga taaccaactg
     6481 agctcttttt cgatgtatag cttttcctga cccattacca cttttactcc gttttattca
     6541 cgggacactg cctggatatt atggcgagca atattggcag atgtccaact atgccagaaa
     6601 agggggaaaa aaactgctgg agcttgcgat ttacaggtga attgaaaaac gccagcggca
     6661 gagctggcgc tttaactacg gggcattact cgtcggcagc gtagccctgc ggcggcagac
     6721 gttgaccatc cagccacgcg gcgttttcgt gcattttcag acgaccatcg gcaaaccagc
     6781 taatcaccag tggataaata gcgtgttcct gggtttgcac gcgggcggtg atgtcatcct
     6841 ccgtatcacc agcaaatacc gggactttcg cctgtaaaat aaccgggcca ccgtccagtt
     6901 catcggtgac gaaatgcacc gatgtaccgt gctcttcatc gccattttcc agcgcctgac
     6961 gatgggtgtg taatccggga tatttcggca gcagagaagg gtgaatgttc agcaaacgcc
     7021 cggcatagtg ggagacaatc gccgggctga gaatgcgcat aaaaccagcc agtacgacca
     7081 catcgggtgc gtacatgtcg atttcatgaa tcaactcccg gtcataggct tcacgactgt
     7141 caaacgcgct ggcgatgagc gtatgcgttg caatacccgc ctggcgggcg cgttcaaggc
     7201 cgaacgcgtc ggccttattg ctgaaaactg cccgtacggt gcctttaatt ttattggttt
     7261 tgcaggcgtc aataattgcc tgcaaattac ttccgttgcc ggaaataagc accacaatat
     7321 tcattattcg ataaccacgc gttgttcgga atcagaggct ttgatgatac cgattttcca
     7381 cgcgttttca ccgttggcat tgagcagggc gagggctttg tccacttccg gagcaggcag
     7441 ggcaataatc atcccgacgc cgcagttgaa ggtgcgatac atttcatggc gctcaacgtt
     7501 acctgccgtt tgcagccagt tgaacacttc cggccactgc caggatgatt catcaatcac
     7561 tgcctgagta ttatctggca atacgcgcgg aatgttttcc cagaagccgc cgccggtcag
     7621 gtgcgcaata gcatgcacat cgaccttttc aatcaactcc agcacggact tcacgtaaat
     7681 gcgggtcggt gccagcagat gatcggctaa tggcttacca tcaagttcgg tggtttgcgg
     7741 atcacaaccg ctgacttcaa gaattttgcg caccagcgaa tagccgttcg agtgcggacc
     7801 gctggagccg agtgcaatca gcacatcgcc atcgctgact ttagagccgt cgatgatttc
     7861 tgatttttct accacgccaa cgcagaaacc agcgacatcg taatcttcac cgtgatacat
     7921 ccccggcatt tctgccgttt cgccacccac cagtgaacag cccgattgca gacaaccttc
     7981 cgcgatgccg ctgatcaccg ctgaagcggt atcaacatcc agttttccgg ttgcgtaata
     8041 gtcgaggaaa aacagcggtt ctgcaccttg caccaccagg tcattaacgc acatggcgac
     8101 cagatcaata ccaatggtgt cgtgacgttt taagtccatc gccagacgca gcttggtacc
     8161 tacgccgtca gtgccggaaa ccagcacggg ttcacgatat ttttgcggca atgcacacag
     8221 cgcaccgaag ccgcccagac cgcccatcac ttccggacga cgcgttttct tcactacgcc
     8281 tttgattctt ccaaccagag cattacccgc gtcaatatca acaccggcat ctttgtagct
     8341 aagagaggtt ttatcggtca ctgcttgggt ccccacgcgt tacttgcggt agaaaaataa
     8401 aattcggcgc aattctaaca gggaaagcaa acgtttgcga gactgcttta cacaaccttt
     8461 ttgcacgtct tttccccagg cgcgcggcga aagaatactt gtaccagggc aaaggatcgt
     8521 tttcggatgg aataatcttc ttctataacc atttgaatat aaaataactt tatctcaaac
     8581 cgttatcatt ttgactaaag tcaacgaaaa taatattgcc gccttgaaga aaggaggtat
     8641 aatccgtcga ttttttttgt ggctgcccct caaaggagaa agagtatgaa gatcgtggaa
     8701 gtcaaacacc cactcgtcaa acacaagctg ggactgatgc gtgagcaaga tatcagcacc
     8761 aagcgctttc gcgaactcgc ttccgaagtg ggtagcctgc tgacttacga agcgaccgcc
     8821 gacctcgaaa cggaaaaagt aactatcgaa ggctggaacg gcccggtaga aatcgaccag
     8881 atcaaaggta agaaaattac cgttgtgcca attctgcgtg cgggtctggg gatgatggac
     8941 ggtgtgctgg aaaacgttcc gagcgcgcgc atcagcgtcg tcggtatgta ccgtaatgaa
     9001 gaaacgctgg agccggtacc gtacttccag aaactggttt ctaacatcga tgagcgtatg
     9061 gcgctgatcg ttgacccaat gctggcaacc ggtggttccg ttatcgcgac catcgacctg
     9121 ctgaaaaaag cgggctgcag cagcatcaaa gttctggtgc tggtagctgc gccagaaggt
     9181 atcgcagcgc tggaaaaagc gcacccggac gtcgaactgt atactgcatc aattgaccag
     9241 ggactgaacg agcacggata cattattccg ggcctcggcg atgccggtga caaaatcttt
     9301 ggtacgaaat aaagaataaa aataattaaa gccgacttta agagtcggct tttttttgag
     9361 taaagcgcct ataacacata atacagagga taatactatg acgcgccgtg ctatcggggt
     9421 gagtgaaaga ccgccacttt tacagacaat cccgcttagt ttgcaacatt tgttcgccat
     9481 gtttggtgca accgtcctgg tgcccgtctt atttcatatt aacccggcga ctgtactgtt
     9541 atttaacggt ataggaacac tgctgtatct cttcatctgt aaagggaaaa ttccggctta
     9601 tcttggttcc agctttgcct ttatttcacc ggtattgtta ctgttgccgt tggggtatga
     9661 agtcgcgctg ggcggcttta ttatgtgcgg cgtgctgttc tgcctggttt cttttatcgt
     9721 gaagaaagcg gggaccggct ggctggacgt gctgtttcca cctgcggcaa tgggcgcaat
     9781 cgttgccgtc atcggtctgg agctggcggg cgtagctgcc ggtatggcag gtttactccc
     9841 ggctgaaggg caaactccag actccaaaac catcatcatc tccattacca ccctggcggt
     9901 tacggtttta ggttccgtgc tgtttcgtgg tttcctggca attatcccga ttttaattgg
     9961 cgtgctggtg ggctacgcgc tctctttcgc aatgggaatt gtcgatacca cgccgattat
    10021 taatgctcac tggtttgcgc tgccaactct ctatacgccg cgcttcgagt ggtttgccat
    10081 tctgactatt ctgccagcgg cgttagtggt tattgccgaa cacgtagggc acctggtggt
    10141 aacggctaat atcgtcaaaa aagatctgct gcgcgatcca ggtctgcacc gttcgatgtt
    10201 tgctaacggc ttgtcgaccg tgatctccgg cttctttggc tccacgccaa ataccaccta
    10261 cggggaaaac attggcgtga tggcgatcac ccgtgtttac agtacctggg ttatcggcgg
    10321 ggcggcgatt ttcgctatcc tgctttcctg cgtcggtaaa ctggctgctg ctatccagat
    10381 gatcccattg ccggtgatgg gcggcgtttc gctgctgctt tatggtgtca tcggtgcttc
    10441 cggtattcgt gttttgatcg aatcgaaagt ggattacaac aaagcacaga acctgatcct
    10501 gacttccgtg attttgatca tcggtgtcag tggcgcgaag gtaaacatcg gcgcggcgga
    10561 gctgaaaggt atggcgctgg cgaccatcgt cggtatcggc ttaagtttga tcttcaaact
    10621 gattagtgtg ctgcgccctg aagaagtggt gctggacgca gaagacgcgg atataacaga
    10681 caaataatgt accgaccggg cagtgttgct gcccggttca aacatcatgg gattctgtgg
    10741 taaacttctc gcgattttgt gaaatcctgg ttgaggtatc tctgaacaca ccggcacagc
    10801 tctctttgcc actttatctt cctgacgacg aaacctttgc aagtttctgg ccgggggata
    10861 actcctcttt actggccgcg ctgcaaaacg tgctgcgtca ggaacatagc ggttacatct
    10921 atctctgggc acgcgaaggc gcggggcgca gccatctgct gcacgcggct tgcgcggaat
    10981 tgtcgcagcg tggcgatgcg gtgggctatg tcccgctgga taaacgcacc tggtttgttc
    11041 cggaagtgct cgacggtatg gagcatttgt cgctggtctg tatcgacaac attgagtgta
    11101 ttgcaggcga tgagttgtgg gagatggcga ttttcgatct ctacaatcga attctggaat
    11161 cgggcaaaac acggttgtta atcaccggcg atcgtccacc gcggcagttg aatctgggat
    11221 taccggatct cgcgtcgcgt ctcgactggg ggcagatcta caaattgcag ccactttctg
    11281 atgaagataa gttgcaggcg ctacagttac gcgcgcgttt gcgtggtttt gaactgccgg
    11341 aagatgtggg gcgtttcttg ttgaagcggc tcgacagaga gatgcgcacg ttatttatga
    11401 cgttggatca gttggatcgt gcgtcgatta ccgcgcaacg taagctgacc attccgtttg
    11461 tgaaagaaat tctgaaactg tagacaagac gtttattgtc ggatgcgcac ccatagtatt
    11521 cgtcggccgg ataagacgcg gcaagcgtcg catccggcaa tgtgcttcaa ctagcgatgg
    11581 agcacttctt aacccacgat ttccagcacc tgctccggtg gacgaccaat tcgcgctttg
    11641 ccattcgcga ccacaatcgg gcgctccatt agcttcggat tatccaccat tgcctgaatc
    11701 agcgcctctt cgctaagcga actgtcagcc aggttcagtt ctttataaag atcctctttc
    11761 tggcgcatca attcacgagc gctattcatc cccagcattt tcagcaaatc acgcagcgtt
    11821 gccgcatcgg cgggtgtctc aaggtagagc accacttctg gctccacgcc gttttctttc
    11881 agcaggttca gcgtttcccg gctctttgaa cagcgtgggt tatggtagat ttttacctgt
    11941 ttggtcattg ttcttccttt aatgcgaatt acatcttggt ataaggctta aagcgttcct
    12001 gcagctggcg caactggtcg atgcgcgcat cgtaacgtgc ttgttgcagg ctgcctaatt
    12061 tcacctgcga actggcgcta ctcaacaagg aaatagcctg atcgagtcgc ccggcgagcg
    12121 cataaccttc tgctcgcgca gccagttcct gatcgcggtt atttagcgcg gcttccgcct
    12181 gtgccagcaa atcccagccg ttgctgtcat ctttattatt aaaggtgtag cgattcagaa
    12241 tattggccgc ttcttgtggt tgaccgcctt gcagataagc gttcgccagg ttgagctgca
    12301 acaccggatt ggtgcgtaaa tcacgggcat ttttcagacg attgatagcg tcattggctt
    12361 tgttttgccc aagatcgata tcagtagcca gatcgagata ccatgcgttg ccaggttctg
    12421 ccgccagtaa cggttgcagc gttttacgcg cctcgtcgta tttattggct tccatcgcct
    12481 gtaaagcacg accatattgt gctgctcgtt gctgacgaac gtttcctttc gcccattcat
    12541 ccagcaaatc actggtgagc tggttacgtc cggaattata catccccagt gtgcgcgctt
    12601 tcgccagata gaaatcttcc gacgactgca ccaccatcgg acgcatctga ttagcacggt
    12661 tgcgggcatc tgccagacga ctttccggca acgggtgagt caataaaatt tccggcggac
    12721 gcgaggagta acgcgcctga tcgagcaatt tttcgaggaa ggtcggcatc gcctgcggat
    12781 cgaatcccga gcgttgcagc acctgaatac caatgcggtc cgcttcctgt tcattttgct
    12841 gggtgaaact gatcatcccc tggcgcgttc ccgccagtgt accggtcagc gcagccatcc
    12901 ccgcctgcgg actggccatc gccagtaaaa tagaacctaa tgcgccgacc caggtcagcg
    12961 gcgcgctgcg ctgctgatct tccatcgctc gcgccaggtg acgttgggtg acgtgggaga
    13021 tttcgtgcgc cataactgaa gccagttgac tttcgttatc ggaataacgg aacagggcag
    13081 agtgcagcac cacgttgccg ccaaagaaag caaaggcgtt aatttcgtcg ttgttgatca
    13141 gaaagaaatg aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc atacccagcg
    13201 aattaatata ttgcgttaac agcgggtcat taattaacgg cgcgctgccg cgtagctggc
    13261 ggacataata gtcgcccatc tgcatttcct ggccaatgga aagcgtgctt cctgcggagg
    13321 ttcccatatc cggcaaggtg tctgcgctgt cggcaaacgc cggggctacc tgaccaatgg
    13381 tcatagcagc aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac ataacctcta
    13441 tcctgtattt ctgaagatag tcatttgacc gatactgtga aatatcgttc ccaccgcgcc
    13501 gcctttgagt gtaacggcga agattagccc actcaaatga cttttcagtt tcctgaatcc
    13561 gtcataaaaa gcgaagtctt ttttgtgaca tttcgataca attgcggatc gcaatcccgc
    13621 tcttgaggtt cagggaaggt ctttatgctc gaaatgttga tgcaatggta tcgccgccgt
    13681 tttagcgacc cggaagcgat tgccttgctg gttattttag ttgccggatt tggcattatc
    13741 tttttcttta gtggcctgct tgctccgttg ctggtggcta ttgtgctggc ctatttgctg
    13801 gaatggccaa ccgtgcgcct gcaatctatt ggctgctccc gccgctgggc gacgtcgatt
    13861 gtattggtgg ttttcgtcgg tatattgcta ctgatggcgt tcgtggtatt gcctatcgcc
    13921 tggcagcagg gcatctactt aattcgcgat atgccgggga tgctcaataa gctttctgac
    13981 tttgccgcca cgttgccgcg ccgctatccg gcgttgatgg atgcgggcat tattgatgca
    14041 atggccgaaa atatgcgcag tcggatgctg accatgggcg attcggtggt gaaaatttcc
    14101 ctcgcctcgc tggtcggttt gctgaccatt gccgtctatc tggtgctggt accattgatg
    14161 gtcttcttcc tgctgaaaga caaagagcag atgctgaacg ccgttcgccg ggtgctgcca
    14221 cgtaaccgtg gactggcagg acaggtgtgg aaggagatga atcaacaaat caccaactat
    14281 atccgcggca aagtgctgga gatgatcgtg gtggggatcg ccacctggct gggattctta
    14341 ctcttcgggc tgaactattc gctgctgctg gcggtgctgg tcggcttctc ggtacttatt
    14401 ccgtacattg gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct attccagttt
    14461 ggtgctggca cggaattctg gagctgcttc gcggtgtatc tgatcattca ggcgctggac
    14521 ggcaacctgt tagtacctgt gttgttctcc gaagcggtta acctgcatcc gctggtcatt
    14581 attttatcgg tggtgatctt cggtggtttg tggggattct ggggcgtatt cttcgccatt
    14641 ccgttggcga cgctgatcaa agccgtgatt catgcctggc ccgatgggca aatcgcgcaa
    14701 gaataatctt cagcccgacg aactgtttcg ccgggctgat tttttagggt tcctgacgta
    14761 aataaatagc ccgataacaa atactcacca gcactgcacc gccgataata ttacccagca
    14821 tcaccgggag cagattggcg gtaataaaat ggctgaccgt cagtgccgga aaattgtctg
    14881 cgctactgtg cgccagttgc cagaaggggg gaggggcgaa atggcgaatg gcaatggcga
    14941 acggaatcac aaacaaattg gcgatacagt gctcaaagcc acttgcgaca aacagggtga
    15001 tgggcaaaat catggcgacg attttgtcgc ataatgaacg tccgcaataa ctcatccaca
    15061 gcgccaggca aaccatcaca ttgcacataa tgccgaggct gactgattca gtaaatgtat
    15121 gatgcatttt gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg ttttcactca
    15181 tcaccagccc ggaaaaccag attaacaaac tgaaacaggc aatacctgcc agattaccgc
    15241 aggccaccag aagtgcatta atcagccatg ttcgccagct aataacgccc cgactttttg
    15301 ccatcaccgt cattaccgac gaggtgaaca ggctggtgcc gcaaaccgct agcaaaataa
    15361 agcctagtgt aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa gacggggcat
    15421 cggcgataac ggaaaggtaa aaagtaaatc caatcgccat aaacacgcca gcggacatcg
    15481 ccattaaaaa gaccgatact ttgctgcggg caattttatg ggcagcaatc cgttcagcga
    15541 ttgccgcttt tctggcgctc aactgcaagt cgaaagagag tttgtttctc atgcatactc
    15601 ctgcaaaaac agattacaac acctcgcgaa ccgagatccc cagccgctgc attcgtgaca
    15661 gcagcgtggt gcgcttcatc cctaatcgcg tcgccgcgcc acggggcccg gcaacaatgc
    15721 cattggtttc tcgcaatacc tgaacaatgc gctgacgttc ttcttcgtca ttctctggcg
    15781 tcgtcgggtg cagcaactgt gccattgaac tgcgaagcgc tgaatcttca tttagcgtcg
    15841 gcagtaaacg gctttgtcgg acattaagat gtaaattcag actgttaccg cgagtcagta
    15901 gtaccgctcg ctcaatcacg ttttccagct cgcgcacgtt gcccggccaa tcccacgaca
    15961 tcaactggcg tagtgcctcg gtcgggatgg cgtcaattgc gcgattcata tggcgcgcca
    16021 ttttttgcgt gaaatgtttt gctaaaagag ggatatcttc cggacggtcg cgcagcggcg
    16081 gcaattccag tgggaagaca ttcaggcgat aaaagagatc gctgcgaaac tggcgatctt
    16141 caaccatttg ccacaaatca cggttggtgg cggcaatgac tctgacattc accgggatcg
    16201 ttctactccc gccgagacgc tcaatctccc gttcctgcaa tacgcgcagc agtttaggct
    16261 gaagttctaa cggcagatcg ccaatttcat cgagaaacaa cgtgccgcca tcggcaattt
    16321 caaaacgacc acgatgggta ttaatcgcac cagtaaatgc ccctttgtca tgaccgaata
    16381 actcactttc cagaagactg gcggggatgg cagcgcagtt gattttgacc agtggcttgt
    16441 cgcgtcgtgg gctaagttga tggatcgctc tggcgatcac ctctttgccg gttccggttt
    16501 caccacaaat caacaccgta ctgacgctct tcgccacaat atctacctgc tggagcaaat
    16561 cttccattgc ctggctttga tagataatgt caccgacgcc cagattcgaa aggagctgct
    16621 cgctaagctg gtggttttct tgctgcaagc tttcctgcaa atcggtcatg ctgcgccagg
    16681 catcggcatt gccaacggca atagcgatgc gatcggcaat atgttgtagt agctggcagt
    16741 tttcctcact aaagagagtg gaagaggtat gcgccagcaa caatgcgccc ggtgtatggt
    16801 tgccaaaggt gagcggtatc aggagcgcag attcgcagcc gttagatgca agtaagtgta
    16861 ataacgggtc gcgttgccag agaaacagat cgtctgcacg gtgcgtcaag gtcggttgtc
    16921 gggtttgtag cgtttgtgtc agcaatacac tttcgccagg catacagcgt ggcagacacg
    16981 cacaatgtga ggcagaaaga tcgctgcacc acaggctgaa cttctcgttc tttcgatgat
    17041 cgcccagtac catactgact gaagccagac cgaaaaaatg atggatctca cgagcgacgt
    17101 cagcgatcag atcgtcgaga tcaagatgtg acaacactgc attagtgata tccaccagta
    17161 cccgatgatg atcgcgatct ttctccagca aacgcagcgc cacctgctgt tcttcatcat
    17221 tgagccatga ttgcagaacc ggagtgatta tgtgtgccag ccagttaaac agtgtgcaat
    17281 cctggggagt aaaacggctg ccgtgagtgc gaataaagcg tagctccgcc attccactac
    17341 tgccgagaag gacggcgtga cgcgaataat cgaccatctc accctgatac tctgttgttg
    17401 ccgtcacatc gctgccatca tcatggtagc ggtaatagaa gttgcttttg agaacatgca
    17461 gttcaataag ttgtaccggg ctgaacggcg gcggcagcgg gatgaaggcg cgtaataaag
    17521 acgccttgcc gtgtttctgc gctaaccgct ccgcgagcat tccgtttacc gcttcaattg
    17581 ttattccttg cggcggggca aacatcgcct cgtctgacat agccatcaac agtccttatt
    17641 aatgagaaat ccttctcacg caatttttct ggccatcaga tagatggccg gttcctgcac
    17701 aatttcatcc aacagatgaa tgaattgaat gctacgcgcc tgccacgccg acgtcgcgtc
    17761 atctgccagt ggcgcgaatg cccgggtcaa cagggcggtg tggctggcgt caataacaat
    17821 ctcaccaaaa gtcatcaccc ccagcatctt acgtcgggct tgctcctctt cgaccagtgc
    17881 aagccaatct tcgtattccg tcagtgggca gcggaaggcg acattcagac aatcaatcac
    17941 gccaacgtga tggccgatag ccaacgagta atacattacc tgggagctgt gttccggcat
    18001 ctcgtcgcta ctggcgataa atttttttcg cagcatccag aaaacaattt ccctgcactc
    18061 ctcagccatg taacccccgg agaacgcgaa agagttgctg aacgatacta ttgagacgcg
    18121 gatcgtcggc ttcgcgcaac caggtgttaa cccgatttcc tgtgggatcg tcgcttaaat
    18181 ggcgcaggag tcgatcgcaa atttctcgcc cttgacgata gcccgccagc cgcctcgctt
    18241 ctcgctcaat ggcgatacgc tgtgatggcg ggatctgcgg ccacagcggt tgcatggtca
    18301 ccccggtggg atcgcgataa tccaccgcgt gaatcttctg ttgcagcaaa ccgagcgcca
    18361 cggcgaaacc gtgaatggtg gcggccggtg ttggcgggca gccggggatc caaacatcaa
    18421 tggggacaat ggtgtcgctg ccgccccaga cgctgtagag atcgtggaaa ataccgccgc
    18481 cgacaccaca cgcgccgtag gaaacacaga ttttatgatc gggggcagac tcatatgccc
    18541 gaagtgcagg catacgcatc gcccgggtga ctgcgccagt aaataacaaa atatcggcgt
    18601 gacgcggtga tgaaacaacc ttaatgccaa aacgttctgc gtcgaatacg ggtgtgatgg
    18661 cggcaaaaat ttcgatttcg caggcgttac agccgccgca atcgacgcga taaacgtagg
    18721 ccgagcgacg gatatcctgt agcaaatgct gcttcatctt ttgcgtttgc tcgtccagcg
    18781 ttatgggctg gctgacatgt tgcgtaagca ctggactcat agctgctcct tagccaccag
    18841 cggttcatct gtgtcgtcgt tgatcagcgt cgcgcgttgt ttgcattccg ggcagacgct
    18901 cgcttgcgcc cgcaacattt cgcggttttg tggtgcattt tgttgctgtg ccaacagttc
    18961 tgcagccagt gcgacggttt tttgcggggc aaacgggcgt tcgcaacggc tgcaacgttg
    19021 tagatggaac gtcgcgcggg tatagagatc ggctttattg gtgacggtca gttcaaagtt
    19081 attggtaagc tgaatggctc tggtcgggca cacttcttca caacgtccgc agtaaataca
    19141 acgtcccaga tagagctgcc aggtgcgcgt attttgctgg tagtcggtct ggatagtcag
    19201 cgcatttgcc ggacaagcac aggcgcaggc accgcaggca atacattggc tgggcatcag
    19261 gtccggtttc ccgcgaaagc ctgggctgac ctccagtggt gcgaagggat atttcaccgt
    19321 cgcggttccg gcgcgcataa tagttttcag taacttcagc atcttctgtc cttacttcag
    19381 cggcgaacgg ttacgatcaa tgccgtagcg ttcgatctct ttatacggca cgatttttga
    19441 ctggcgcttg cgcacatcaa ccagcgtcac acggtcggta caggagtagc agggatcaag
    19501 gctaccgata attagcggtg cgtcagaaac ggtattgccg cgcagcatgt aacgcagcac
    19561 tggccagttg gcgtaggtgg cggcacgaca gcgccagcgg aacaattttt ggttatcacc
    19621 gagcatgctc cagtgcacgt cttcaccacg tggcgcttca acaaagccca gcgcgaatgc
    19681 gtgaggttta tagctaaagc cttcggtcag cagtggggta tctggcatgt tgtcgagggc
    19741 aaattccagc attgccagcg aatcaaacgt ctctttgaca cggaccatca cgcgggagaa
    19801 gacatcgccg ccggtaaagg taaacagcgt ttttggaata ttgccgtagt cggcgtaggg
    19861 gtgatcaaag cgcaaatcac gggcaaaacc actgccgcgg atcagcggcc cgacagggct
    19921 ataatcacgg gcgatttgtc ggtcgagaat gccaatgccc tgagtgcgtt gttccatatt
    19981 cggcgtggca agcagcatct ctaccagctc cgacacgtcg gcgcgcatct cgcgcactag
    20041 tttcagcgtt tgcagacgtt gctctttgag aatatcgcgg cgaacaccac caatcagatt
    20101 cagaccgtag gttttacgcg acccggtcag caattccgcc atcgtcatcg acttttcccg
    20161 cacgcggaaa aattgcataa agccggtatc aaaaccaaca aaatgacagg agaggccaag
    20221 gttgagcaaa tgactgtgca gccgttcgac ttccagcaga atcgagcgaa tagtatgcgc
    20281 tcgttgcggc acctcaatcc ccagtgcatt ttcaaccgag ttggtatagg ccacactgtg
    20341 ggcaaaacca caaatcccac acacgcggtc ggataagaag gtcacttcgt tgtagcccat
    20401 ccgcgtttct gccagtttct ccatgccgcg atggacataa aacaggcggt aatcagcatc
    20461 gacaatttgc tcgccatcca caaacaagcg gaagtgacct ggttcatcgg aggtgatatg
    20521 cagcgggccg acagggatca cccgcgcatc gctgttgccc tcgttgataa acggatacgt
    20581 ttcggtatca gtcgtcggtt ctgggcgcag tcgataatcc atcgcgtctt tgcgcagcgg
    20641 atgcatatct tccggccagt catcgggcaa caccagccga cgctgatccg gcaggccaac
    20701 cggaatcagc ccgtacatat cgcgaatttc tcgctcgccc cagaccgcgg cagggacgcg
    20761 cggtgtgact gacggaaact cccgactgtc ggcatcgacc agtgccttca ccacgatcca
    20821 gcatttttcg gccccttcca tagaaagggc gtaataaacc gcgtaatgac cgttaagtgt
    20881 ccgctcgtcg ttgccaaaca ggaccggaag ccagccatca tgttgataat aaagataatg
    20941 tacaacgtca ggcagcagat tgattttcac cgtaatggtg acctgttcag gcgtttgtcg
    21001 ctcttcatcc agcaccgcgc cggggaactg cgttttcagg gcggcgagaa tcgcttcgcc
    21061 acgatttgac gatgaattaa cgttcactgt gtttctccga aacagatgcg gtgcctgaag
    21121 gcaaaaaatc atgccaggtg ctacgttgtg caggcaggtc gtgcgtccca gagaggacta
    21181 tagtggaagc gcccgccagg atcctgatga caggttgtgg aatatgcgtt cccatcgcca
    21241 gcatcatgac cagcagaatc accattggcg aggtggtcaa ccagccaagt tcaccccggt
    21301 taacggcctg cggcggtttc gccattaata cccgtgcagc catccgtacc aggcccgcca
    21361 gcaccagcgt taacagcaat aacagcagga cgataagcag cagatgatta cgcgccagtc
    21421 cggcggtaac ggtcataaat tcgctaagaa aaatgttgaa gggcggcatc ccggccagcg
    21481 ccagcgcacc gccgccaaac aacacggcgg taaatggcat gattttgagc atcccacaga
    21541 cgacgttgag atcgcgcgtg ccgtacttga gcagtacatt gccggaaccg cagaacaaca
    21601 gcgttttggc cagactgtgg tttaaggtgt gcagcagcgc ggcaaaaatt cccagcggcc
    21661 cgccaatgcc taacgccacc gcgaccagtc ccatgttctc cacgctggag tacgccagca
    21721 gacgcttaat gtcccgctgt accagaatga aaaatgccgc cacggcaacc gacaacatgc
    21781 cgaagatgag caacaaccgg ttggggaaat cgctgccgat ggcctggcaa ataatgatgt
    21841 aatagcgaat aagcaccaac agcgcgcagt tcagcaatac ggcggagagc agggcgctga
    21901 ccggactcgg cgcttcactg tgagcatctg gcagccaggc gtgcatgggg aatagcccgg
    21961 ttttggtgcc aaagccaatt agcacaaaca caaaggccaa cagcattaat gttgggtcaa
    22021 gcaaggacga ttgcttaaga acctcgctcc agaatatcgc catttctgcc tgcggcataa
    22081 cgctggcggc gttggcgtat accagcacgg taccgaacag accaaaagcg acaccaacag
    22141 tacaaataat gatgtacttc catgcagctt ccagcgatga acgctgaccg taaatgccta
    22201 ccagaaacgc cgagcttaag gtggtggctt caattgccgc ccacatcaca atcaggttat
    22261 tgctggtaac aaccagcagc atggtgaaca aaaacagatg gaagaaaccg tagtaatcgc
    22321 acagcgtaac gggtgaaagc tcgccgtgtg ccacttcatg acgcatgtag ccaatcgagt
    22381 aaacaccggt gagaaagcca atcacaccaa gaatggcgag gaacaaaccg cccagaccat
    22441 caatgtgaag ccacagtccc gcagcgaata tttctcccgc atcagcggca gtttggacca
    22501 cccacagtgc cagaatcagc agcagtgtga tccctaagcc atgtagtact gtcaccgtgc
    22561 gagtcgcaga gtgcccccgt ttccggcagg caaaacagag cagcgaaaaa agcagcggcg
    22621 tgagcaggag taaagcgaac atcacagaat aactcatctc attatccctt cagcgcggtc
    22681 aagttattca cgtccagcgt gccgtgggta cgccatattt ttcttgccag taacaccatc
    22741 acaatgacgg cgaagatggc gtcggtagcg atacctattt ccaccagttc cggtgctcgc
    22801 caggcaagaa gcgccagcac cagatgggag ccgttttcca tcaggcagta accaaaaatt
    22861 tgccgcagga tattgcgctg gctgacaatg cacagcaggc caagcagaaa atgacctaac
    22921 gccaccgcca gcgccggttt cagcccggta gccatcggta gcttcacggg ctgaacgaca
    22981 aatgcgcaaa gcaggacaat taacgccgcg agcagtgtca tcattgccgg accgaataac
    23041 gctttttccg ggatgttctg gggaatattt cgtgcagcgt aagtcatgat taacggtacc
    23101 agcagcactt tggtgataaa ggcgctggcg gaccagatca gcagttgctc tgcgacgaac
    23161 aggcacgaga gggtggcgaa aatagacacc agcaccagcg actggcaggc gtaaaatccg
    23221 catgacaggc gatagctttt gacgctaatc acaaacagcg atgtcagcat catcagtccc
    23281 gccagattat ttacgatcat agaaccggtc attcatcatc tccttactgc aaccaggtcg
    23341 cggcgattac gctggaaatc agcgacatca caatcagtac aatcaacacc aggcgcattg
    23401 atcctggcag cggtgcggca tcggcgacgg cctcgctcgg tttgccaggc acgacgcgac
    23461 caaaccagcg aataaaccag gcgaaactgg cgaccgattc aatcatcagc agaatcatgg
    23521 cgggcagcag gatccagtac tccactgaca acgcaaaacc ggcggcaaac agcgggaatt
    23581 tactgaagaa gccgttgaac ggcggtacgc cggtaatcgc cagcgctgcc acgcagaaac
    23641 ccacgcctgg caacggcagg gtgtgcaata cgccacgcag acgcggcaac aagcgcgtgc
    23701 cgcagctgta actcagcgca cctgctacaa ggaaaaatag gcttttagcg aacgcgtggt
    23761 tgacgatgta ggcgatgctg ccctccagcg ccagccgcga gccgaagatg gagagcgaca
    23821 agccgaaaaa catccagcca agttgagtga tggtcgacca ggccagcaac cgcttcatat
    23881 cctgctgtgg caaatacatc aggaagccat aaagaatggt gaccagtgcc ataaccatgc
    23941 caacgccgcc aatcacatgc gggatattgc cgccgtcgat aatggcgcgg gcaaaaatgt
    24001 aaacgcccac tttcaccatc gatgcggcgt ggagataggc gctgatcggt gttggcgctt
    24061 ccattgcatc cggtagccac gcttgcatcg gtagctgggc cgatttcccc cacgcggcaa
    24121 acaggatgcc accataaacc agatagcgtg cgtcgccgtg taactcgctc atcgcgctga
    24181 gcgcgaacgt tccggtctgc aaaaacagcg tggcggcggc aagatacaac cccaacgaac
    24241 cgatatgagt gataagcaac gcttttagtg ctgaacgctg cgctttatcg ctctggtaat
    24301 aactgatcaa cgcccaggag cagccgcccg taatttcaaa aaacaacaac tgaccgagca
    24361 gcgtcgagga gagtaccagt cccgccatcg cgccgataaa aaccagcaaa aatgcgtaat
    24421 aacgattcgt gccgttatgc gggtgttcgc gatttttatc cgtcagataa cccgtggagt
    24481 agatcgtgac cagcaaaccg agaaacacca ccacaaacag aatcagcgta ctcacgcgat
    24541 caatgaccag gccaaacaac gccacacccc ccacgttgac caacgtcaac gtgacggcaa
    24601 ctttatcggc ctgataaaac gcggagatca gtgacaacat gcacagcgtg gtcagcgcgg
    24661 cgaacaaaac cccccattcg gcggcccgac gttgtggcga aaacgaaacg accagtgcgc
    24721 cgataaaagg cagcaataac gtcgtcagag caagattttc catattccgt cagtgctcct
    24781 tacagaccgg ttaaccagaa gacccatgca agcgcagcaa ggctgaagcc aagccaggtc
    24841 acatggtgaa tgagtaaaaa tcgcccgcgt gccagcgtgt tttctgcgat tgaggccagt
    24901 acaaaaatca gcaaaacctt gagcagcgta acgacaagtg aagtcagcag gcaggcgaga
    24961 gaaagctctt gcgcgcgccc aaagggcaga aacagggcca caaacagtga tgccatcacg
    25021 acctgtttca gccccagccc caacttcgcc agcgccagcc cggcaccgga atattcggtc
    25081 agcgggcctt cctgtaattc ctgttctgct tcagcaacat caaagggaat ttttcccatc
    25141 tcaatgaagc aggcaaaacc acaggccagt aacgccagta cggtggttag cggcgagttc
    25201 cagcccgtcg ccagcgtctt gctgatcatc tcgatatgcg tggaatccgc tatcagcgcc
    25261 aataccagca gtgagagaat gagcatgggt tcgaccagaa tgccgagcgt caactcgcga
    25321 ctggcaccga ctcccgcaaa cgggcttccg gtatccagcc cggaaagagc aaagaaaaaa
    25381 tgaaacaggg caagaagata gataagggtg atcagatcgc cgccgcccgc aaaaggggaa
    25441 acagtaataa atagtggcag ggccatcgcc agcaccagca tgctgctgat taatacccac
    25501 ggcatcaggc ggaacatcag acctgaagat gtcggcgcaa cttcctggcg tttaaacagt
    25561 ttgtggatat cgcgataatc ctgccagaac cccggtccgc gacgggagtg cattcgcgcg
    25621 cgtatctgcc gggaaatccc cgtaaaaagc ggggttagca ccagcagagt cacggcctgt
    25681 gctaacgcaa aaatgaccag atatccgtcg caaagagttt gtctcatggt gatttcctta
    25741 gacggcaata gcgattagca gcacaaccag tgcggcgacc acatacaggc aatagagacg
    25801 aaagtcgcca ctttgcagat gctgtatttc tttggccagc cgttgggtgg cgctcacgat
    25861 ggggcggatc acccgctcat cccagaaggg ttctgtgctc tgagccctgg cggtgacgtg
    25921 cgcaagacct ttgttcagcc tcagcgtagg gtcgagctgt tgtcgtagac gaaatagcgc
    25981 acaaaagacc acacgcagcg gctgcatcac gccattgcct gacggggcca tcgcatgttc
    26041 ccagccgtag ccgcatgccc aggcatctcc agtgcgacga aacgctgcgc ggcgcgaacg
    26101 acaccacagc cagtaaaggc caggcagcaa aggcatcgcc agtagtaaca gcaacagtaa
    26161 tgagggagtg acccgtgtat gaaacgtgcc gggtacaagt gctattccgc tggtgacagt
    26221 gacgggaggg gtattggtaa acgcatgggc aatgtgcatt atcttcggtg ccagccaact
    26281 ggcactaagc gcaatgaata cgcagagtgc cgcgagcagt agcatggcga cgatcatcgt
    26341 atttggcact tcctgtgcct cttcagcgtg tgtactgcgc ggcgcgccac agaaagtaat
    26401 accgtacatt ttgacgaagc acattactgc cagcccacca gtgactgcca gcatcaccat
    26461 agcaatagga cccgcaagtt gtagcgctac ggcttccaca cgacttagtg agaacagcga
    26521 ctgccaggtg taccattcgc tgataaaacc attcagcgga ggaatggctg atatcgcgag
    26581 gcaaccaatc aggcatgctg cggctgtcca cggcatccgt ttcgccagtg cccccatttt
    26641 ttccatgtcg tgggtatgca aacgcgaaat aatcgcaccc gcgccgagaa acagcagccc
    26701 tttgaacagc gcatggttga gcagatgaaa cagcgcgccg agcagtccaa caacggtgag
    26761 cagcgggtcg tgcagtgaca gaccgaccat cgccacaccg accgccagca aaataatgcc
    26821 gacgttttcg acggtgctcc aggccagcag ccgtttgata tcctgttccg ccagcgcata
    26881 cagcacgccc aggagcgcgg agattgcgcc gatcgccatc accagaatgc cccaccacag
    26941 cggcaaaccc gtttgcgcca gcagatcaat cgctactttc aggatgccga aaataccgat
    27001 tttgaccatt acgccagaca tcaacgccga agcgtgcgat ggtgcggcag ggtgagcgcg
    27061 tggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg ccaaaaccga aaaaggccag
    27121 caggaacacc gccgacgcca gccccggaga aagtgaaagc gtgcggaaac tggcaaaatc
    27181 gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc ataatcagca ccgatccggc
    27241 gtgggcgata aaaaagtaga gcatgccagc gttgatcgac gttttatcct gcctggcaat
    27301 gaccagaaac caggaagaca gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt
    27361 gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc
    27421 cgccgcgcct ttaccctcgt attcgcgcat ataagtcaat gaatagagcg aacaaaccac
    27481 caccagcaag gagataacca tcaccataaa tgccgagagg ctatccattc gcagtgttaa
    27541 atcggcaaag tcaaaaggcg tggcaaaacg ggcgactaaa gttacgcctg catgtaattg
    27601 cgtaattccg ctgatgatgc caatcacgcc acccaccagc gatgtgatgc cggaaagctt
    27661 aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc agactggcaa acagatagag
    27721 gatcagtgac caggttaata attgcagggc atccattagc gttgctcctg agtgagggaa
    27781 aggggatcct ccccgccatt ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc
    27841 tgacgttgca ggctatcgtc ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg
    27901 caggccggac cttctggcaa gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca
    27961 cccggttccc agcgcagcaa agcatgttgg gtcggcgcgt tttcttcgcc gtctttgagt
    28021 gagccgtcag cctgaaaaac atattgcgca ggggcattca ccggacggct tcctgaagcg
    28081 ctgattgcgc caaatgggca gaccacggcg caaagcttgc agccaataca gaggctttcg
    28141 ttgagttgga tcgcatcgtc cctctgggag atggcattga ccgggcagac ctgcaggcaa
    28201 ggggcttcct cacagtgatg acacacgaca ggggcagtga gtgttgacgt cttcgccagg
    28261 gccaggcgcg ggtgttgctg taaaccttgc gttttatgca cgtccgaaca ggcagcgaga
    28321 caggtattac atcctgtaca ccacagtggt tcggccacca caaagcggtt catgtcggct
    28381 ccttgaaaat gatatgaaac attgcatttc atattgcata aaggatgcca gtctgattaa
    28441 tttaatgtat tcaataagtt atgaattaac tgtattctga acggtacaaa aatgatggtc
    28501 attaacgttc agcaaaaaat gaattcgaca cccgtgtcgc aggggaaagc gagaatgtga
    28561 tcttgatcac gagaagtgag gtgtgctgac cgcaagcgca gccagcacgg aatggagcaa
    28621 agtaatcagg cgtgttcttt cagccagttc agcacaacgt cgtggtgatt gctggttttg
    28681 aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa tcaggaagct gatgcgatga
    28741 ataccatcgt aggttttgcc catgaaggac ttttcacccc agacgccgaa ttgttcgcac
    28801 acctggtggt cctcatcaga caggagcgta aagttaagca gctctttttc cgcaaaacgg
    28861 gagagttttt cgggtttatc ggtgctgata cccagcacat caacgcccgc ttttttcaac
    28921 tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac agccgggggt catggctttc
    28981 gggtagaaat aaaccagaac acgctgtccc tggaagtcgg tcaaattaac ttgttctccg
    29041 tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg ctttcagtgg attcattact
    29101 taactccatc ctgttcatca tgttgggaat aattgacgac gttaatactg ccttgtgcat
    29161 tgagttctgt acatagggct ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg
    29221 ggctgtgggc ggttatctga atatgcaact gcgcagcccg ttcattttca gcaggttgcg
    29281 tgcgcgacac cagctccgca atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt
    29341 caattaaatg cggggagtct gccacatcga cctgaaccca gacagatgct ggcattggcg
    29401 gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag atccagttct gcacctttca
    29461 acggtaacgt tgattcaatc agagtaatgg cattccatga accggaaagc agcatgataa
    29521 acgtgaactc ttctcccagc atcgccaggc gactgtcttc aatattacag ccgcaactac
    29581 tgacatgacg ggtgatggtg ttcacaattc cagggcgatc ggcacccaac gcagtgatca
    29641 ccagataatg ttgcgatgac agtgtcaaac tggttattcc tttaaggggt gagttgttct
    29701 taaggaaagc ataaaaaaaa catgcataca acaatcagaa cggttctgtc tgcttgcttt
    29761 taatgccata ccaaacgtac cattgagaca cttgtttgca cagaggatgg cccatgttca
    29821 cgggaagtat tgtcgcgatt gttactccga tggatgaaaa aggtaatgtc tgtcgggcta
    29881 gcttgaaaaa actgattgat tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg
    29941 gcaccactgg cgagtccgct accttaaatc atgacgaaca tgctgatgtg gtgatgatga
    30001 cgctggagct ggctgacggg cgcattccgg tgattgccgg gaccggcgct aacgctactg
    30061 cggaagccat tagcctgacg cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg
    30121 taacccctta ctacaatcgt ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg
    30181 ctgaacatac tgacctgccg caaattctgt ataatgtgcc gtcccgtact ggctgcgatc
    30241 tgcttccgga aacggtgggc cgtctggcga aagtaaaaaa tattatcgga atcaaagagg
    30301 caacagggaa cttaacgcgt gtaaaccaga tcaaagagct ggtttcagat gattttgttc
    30361 tgctgagcgg cgatgatgcg agcgcgctgg acttcatgca attaggcggt catggggtta
    30421 tttccgttac ggctaacgtc gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag
    30481 aagggcattt tgccgaggca cgcgttatta atcagcgtct gatgccatta cacaacaaac
    30541 tatttgtcga acccaatcca atcccggtga aatgggcatg taaggaactg ggtcttgtgg
    30601 cgaccgatac gctgcgcctg ccaatgacac ctatcaccga cagtggtcgt gagacggtca
    30661 gagcggcgct taagcatgcc ggtttgctgt aaagtttagg gagatttgat ggcttactct
    30721 gttcaaaagt cgcgcctggc aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc
    30781 tgtagttctg actcacgcta taagcgtcag gtcagtggtg atgaagccta cctggaagcg
    30841 gcaccgcttg cggagcttca tgccccggct ggaatgattt tgccggtgac ctccggtgat
    30901 tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta aggcgctgga cattcgtcca
    30961 ccagcccagc cgctggcact ggtttctggc gcgcgtaccc agttcacggg cgataccgct
    31021 tcattgctgg tggaaaatgg tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg
    31081 caggcgaaaa actacaccat cacccaacgt gatgatgctg gtcagacact gaccaccgat
    31141 tgggtacaat ggaaccgtct ggacgaagac gagcagtatc gtggtcgtta tcaaatctct
    31201 gttaagccgc agggttatca gcaggcggtt acggttaaac tgctgaacct ggaacaggcg
    31261 ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca gcacggagat gatgaacgtt
    31321 atttccgccg gtctggataa atctgccact gacgccgcga acgctgcgca aaatcgtgcc
    31381 tccaccacta tggacgtaca aagtgcagct gatgacaccg gtttaccgat gctggtcgta
    31441 cgcgggccgt tcaatgtggt ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg
    31501 aaagtgaccg acagcacccg ttcgcagggt aacatggccg taacttacaa gccgctgtct
    31561 gacagcgact ggcaggaact gggcgcgagc gatccaggcc tggcatccgg tgactataaa
    31621 ctgcaggttg gcgatttaga taaccgcagc agcctacagt tcatcgatcc gaaaggtcat
    31681 actctgactc agagtcagaa cgacgcgctg gtagctgtct tccaggctgc gtttagcaag
    31741 taaaaataca gggctggagt catccggccc ttttttctga tatgatacgc aaacgtgtgc
    31801 gtcggcagga aaacgcgatt ttagcggtaa ttcgcacgaa atttgtttgt cggacgtcgt
    31861 tcggataaag cgttcacgcc gcatccgaca aaacatccgg cacaccagac agcaaaagat
    31921 tttaaaacgt taattcacac ccaggagtga taaagatgca aaagcaagct gagttgtatc
    31981 gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga cctgttggtg ctcgaattcc
    32041 gcaatgatac gtcagcaggg gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg
    32101 tgaacaacaa gttcaactac ttcattatga gcaaactggc tgaagcgggt atcccgactc
    32161 aaatggagcg tctgctctcc gataccgaat gtctggtgaa aaagctggat atggtgccgg
    32221 ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag
    32281 aaggtattga gctgaacccg ccgctgttcg atctgttcct gaaaaacgac gccatgcacg
    32341 atccgatggt caacgaatct tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg
    32401 cgcgtatgaa agagctgacc tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg
    32461 ctggtctgat tttggtcgac tttaaactgg aatttggcct gtacaaaggt gaagtggtac
    32521 tgggcgatga gttctccccg gacggcagcc gcctgtggga caaagaaacg ctggagaaaa
    32581 tggacaaaga ccgtttccgc cagagcctcg gtggcctgat cgaggcctat gaagccgtcg
    32641 cccgccgcct gggtgtacag ctggactgat ttttctgctc atcatcttgc cgtgcagctg
    32701 gcacggcaag acaaccgctc tcggaagatg tgcattgaac gctcattcct ccgccatttc
    32761 atcccgcttt cctgtggtaa tcttcctctg aaccacctac gatcatttct ataatgaata
    32821 tattgttgag gtatctatgc gttggcaagg gcgacgtgaa agtgacaatg ttgaagacag
    32881 acgcaacagc tctggtggtc cgtctatggg cggtcccggt tttcgcctgc caagcggtaa
    32941 aggcgggctg attttactga tagtcgtgct ggttgcaggc tactatggcg ttgatttaac
    33001 cgggttgatg accgggcagc cggtttctca acagcaatca acacggtcaa ttagtcctaa
    33061 tgaagacgaa gccgcaaaat tcacctcggt gattctggca accacggaag acacctgggg
    33121 acagcaattc gagaagatgg gtaaaaccta tcagcaaccg aagctggtca tgtaccgtgg
    33181 aatgacgcgc accggctgcg gggcgggcca gtccattatg ggaccgttct attgcccggc
    33241 ggatggcacg gtttatatcg atctctcctt ctatgatgac atgaaagaca aacttggcgc
    33301 ggatggcgat tttgcccagg ggtacgttat cgcccatgaa gtcggtcatc atgtgcagaa
    33361 actgttaggc atcgagccga aagttcgtca actgcaacaa aacgcgacgc aggcggaagt
    33421 gaaccgctta tctgtgcgta tggaactcca ggccgactgt tttgccggtg tctgggggca
    33481 tagtatgcag cagcaaggtg ttctggaaac cggcgatctg gaagaggcgc tgaacgcggc
    33541 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    33601 tttcactcat ggcacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    33661 tgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc agggatggct
    33721 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    33781 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    33841 ttacctggcg actggctgtg gatttcgccg cagccagtcg ctgaaaacca ctgttttccc
    33901 tcggcgctac aaactttact tgggcgcgaa ttccggcatg cggtattcga cgcccgccac
    33961 ggctttgatg ccgctgcctt tgcagcactt agcggaacgt tgaaagcggg aagctggctg
    34021 gtgttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    34081 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgtgta
    34141 cttacggcga ataacgacgc catcctctgg cggcaaaacc agccgttcac gttggtgcat
    34201 tttgctcccc gtactgactg gcaccccgcg accggcacac cacaaccaga acaacagcaa
    34261 ctcttacagc agttactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    34321 cgcggtaaat cggcgttggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    34381 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    34441 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    34501 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    34561 ttgttaacca ctacggtgca gggctacgaa ggcaccggac gtggtttttt gctgaaattt
    34621 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac aaccgatccg ctgggcgcag
    34681 ggatgcccgc tggaaaaaat ggttagtgag gcactggtgt ttgacgatga aaacttcacc
    34741 catacaccac aaggcaatat cgtcattgcc gcatttgaac agacgttatg gcgaagtgag
    34801 ccagaaacgc cgttaaaggt ttatcaatta ttgtctggtg cgcactaccg gacttcgccg
    34861 ctggatttac gccgcatgat ggatgcacca ggtcaacatt ttttacaggc ggctggcgaa
    34921 aacgagattg ccggagcgct gtggctggtg gatgagggcg gattatctca agaactcagt
    34981 caggcggtat gggcgggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    35041 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcgggttag ccggatagca
    35101 gttcatcctg ctcgtcagcg ggaaggcaca gggcgacagc ttattgttgg tgctttgcaa
    35161 tatacgcatg acctcgacta tctttcggtg agttttggtt acaccgagga gttatggcgt
    35221 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    35281 ggttgctata cggcgatggc gctgttaccg atgagtgatg cgggtaaaca gctggctgaa
    35341 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    35401 cttcctgttg atccactaaa cgatgccgtc ctttctgacg acgactggct tgaactggcc
    35461 ggttttgctt tcactcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    35521 caaaccagcg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa cgccagtgac
    35581 gcgcagttat gtaccacact taaactttca ggccgtaagc tgttactggt ccgtcaacgc
    35641 gaagaagccg cgcaggcgct gttcgcactt aatgatgttc gcactgagcg tctgcgcgat
    35701 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    35761 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    35821 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    35881 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    35941 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    36001 ttcggtgcag ggtattacgg aagataatcg ccaggcacgt gtggatgcca tcatgccgac
    36061 gtttattgaa acggtgcgct actggcagaa acagagcgga gtaggggcta acgccacggc
    36121 actaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    36181 ccttgcttcg cgcgtcattg cctttaacgg acgctatgcc agcctgccgg aaaccgcgtc
    36241 tacagccacg acaattcact tgattcatgg tggtgaagat ccggttatcg atctggcgca
    36301 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    36361 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    36421 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcaatga
    36481 tgatgtgatt gaaatgatgt aataataacc gggtaggcct gataagactg tatcaggcat
    36541 catggccgga tgtggcgtga acgccttatc cggccgctca gcttacttct tcggttgatc
    36601 ctttttcggc cagtcgtcgt catcgtccca cttatcgttg aaatcacgat gcgggggaag
    36661 ctccggcttg ttggcgagaa atttcttatg gtcgacgcgc ttgagatctt taatcacgtt
    36721 cagcaggacg cctaccaaaa agacaatcac cagaatccac caatatttag ccagccagtc
    36781 catgcttatt tcctcttgca gaaccactca tcaggcgacg agctgttcca tgatacgttg
    36841 atacatacgg gcaagtagct gcaggtcggc agcgttcaca cattcattaa ttttatgaat
    36901 agtggcattg accggcccga gttccaccac ctgcgccccc atgcgggcaa taaagcgccc
    36961 gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt tcattatagt gctcaaccgc
    37021 gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa aatggctgcc cggaaagcca
    37081 ccattccacc gtataacgca gttgatgttt ttcaagcagg gcaagtacct gcgctttgat
    37141 catctcatca gtcagttcgg tgctgaagcg gaagttaaac tgtacaaaca gttcacccgg
    37201 aataacgttg ttactgcccg ttcccgcctg aatattggca atctgcatac tggtcgccgg
    37261 gaagaattca ttgccctgat cccactcaat agccactaat tcattaagga aaggtgctgc
    37321 gcgatgtacc ggattgtcag ccagatgtgg gtaggcaaca tgcccctgca cgccatgaat
    37381 agtgaggttg caggttaacg atccgcgacg accatttttc accacatcac ctaccacttc
    37441 gatactcgac ggttcgccaa ccaggcagta atcgagacgc tcattacgtg ccatcaacgc
    37501 ttcgacgact tttaccgtac cgttatgggc actggcttct tcatcagaag tgatcagaaa
    37561 tgccagtcgc cccgtatggt tgggatgttg tgcgacaaag cgttctgccg ctaccaccat
    37621 cgccgccagc gagcctttca tatctgccgc accgcgcccg aataacatgc catcacgaat
    37681 ggtgggttca aacggcggat tgatccaacg atcggcgtcg ccaggcggca ccacgtcggt
    37741 atgcccggca aaggccaacg tttcaccttg cccacgccat gcccaaaaat tctgcgtatc
    37801 ggcaaaatcc atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc
    37861 ctggcatcct gcgtcatcag gactcaggga agggcggcga ataagctgtt gtgtcagctc
    37921 aataaccggg cacgacatag actacacctc atggaaaaat tgctgataac tggaatcact
    37981 gaaacccagc agcataggct taccaggcgt gcagagcaat ggacgtttga taattgcagg
    38041 catttcagtc attaatgtcg ccgcagaggc cgcatcgatg attttattgc gggtggtttc
    38101 gtccagttta cgccaggttg taccacgggt gttgagtaac gcttcccagc ctaattcgtt
    38161 gataaaaccg ttcaataatt cgctgtccag cccatcgacg cggtaatcat gaaaacgata
    38221 atcgatgtta
//
LOCUS       DARNVM010000023        38204 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.023, whole genome shotgun sequence.
ACCESSION   DARNVM010000023 DARNVM010000000
VERSION     DARNVM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.023"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..144)
                     /locus_tag="SI621_001145"
     CDS             complement(<1..144)
                     /locus_tag="SI621_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEI4063886.1"
                     /translation="MIDLENQEREIINLMFSQGISWITAVRIRHKLSLAEVSKMLGIS
                     INSL"
     gene            complement(176..1192)
                     /gene="lplA"
                     /locus_tag="SI621_001146"
     CDS             complement(176..1192)
                     /gene="lplA"
                     /locus_tag="SI621_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HEI4063887.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPEITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(1220..1864)
                     /locus_tag="SI621_001147"
     CDS             complement(1220..1864)
                     /locus_tag="SI621_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HEI4063888.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRNDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            1970..2938
                     /gene="serB"
                     /locus_tag="SI621_001148"
     CDS             1970..2938
                     /gene="serB"
                     /locus_tag="SI621_001148"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HEI4063889.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            2987..4369
                     /gene="radA"
                     /locus_tag="SI621_001149"
     CDS             2987..4369
                     /gene="radA"
                     /locus_tag="SI621_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEI4063890.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4390..5622
                     /gene="nadR"
                     /locus_tag="SI621_001150"
     CDS             4390..5622
                     /gene="nadR"
                     /locus_tag="SI621_001150"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HEI4063891.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(5848..6033)
                     /locus_tag="SI621_001151"
     CDS             complement(5848..6033)
                     /locus_tag="SI621_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063892.1"
                     /translation="MPLINRIVMPPMTRSRAGDVATDIMADYYAQRASAGLIISEGTQ
                     ISRSAAHNFPRHADLLR"
     gene            complement(6082..6294)
                     /locus_tag="SI621_001152"
     CDS             complement(6082..6294)
                     /locus_tag="SI621_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4063893.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            complement(6452..6868)
                     /locus_tag="SI621_001153"
     CDS             complement(6452..6868)
                     /locus_tag="SI621_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEI4063894.1"
                     /translation="MAKTTVSIEIPASADAVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            complement(6894..7691)
                     /locus_tag="SI621_001154"
     CDS             complement(6894..7691)
                     /locus_tag="SI621_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4063895.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQRGV
                     DDAMAAIATQTPTASLRGVVADLSTEDGVAALIAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            complement(7711..8721)
                     /locus_tag="SI621_001155"
     CDS             complement(7711..8721)
                     /locus_tag="SI621_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4063896.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     RLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMRRQNLVAENAPLP
                     TR"
     gene            8855..9742
                     /locus_tag="SI621_001156"
     CDS             8855..9742
                     /locus_tag="SI621_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001484212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4063897.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPTDSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     FNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            complement(9897..11564)
                     /gene="ettA"
                     /locus_tag="SI621_001157"
     CDS             complement(9897..11564)
                     /gene="ettA"
                     /locus_tag="SI621_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEI4063898.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVNNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            11775..13712
                     /gene="sltY"
                     /locus_tag="SI621_001158"
     CDS             11775..13712
                     /gene="sltY"
                     /locus_tag="SI621_001158"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HEI4063899.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            13802..14128
                     /gene="trpR"
                     /locus_tag="SI621_001159"
     CDS             13802..14128
                     /gene="trpR"
                     /locus_tag="SI621_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HEI4063900.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(14122..14637)
                     /gene="yjjX"
                     /locus_tag="SI621_001160"
     CDS             complement(14122..14637)
                     /gene="yjjX"
                     /locus_tag="SI621_001160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HEI4063901.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            14689..15336
                     /gene="gpmB"
                     /locus_tag="SI621_001161"
     CDS             14689..15336
                     /gene="gpmB"
                     /locus_tag="SI621_001161"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HEI4063902.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(15333..16202)
                     /gene="robA"
                     /locus_tag="SI621_001162"
     CDS             complement(15333..16202)
                     /gene="robA"
                     /locus_tag="SI621_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HEI4063903.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            16413..16886
                     /gene="creA"
                     /locus_tag="SI621_001163"
     CDS             16413..16886
                     /gene="creA"
                     /locus_tag="SI621_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HEI4063904.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16899..17588
                     /gene="creB"
                     /locus_tag="SI621_001164"
     CDS             16899..17588
                     /gene="creB"
                     /locus_tag="SI621_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HEI4063905.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            17588..19012
                     /gene="creC"
                     /locus_tag="SI621_001165"
     CDS             17588..19012
                     /gene="creC"
                     /locus_tag="SI621_001165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEI4063906.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            19070..20421
                     /gene="creD"
                     /locus_tag="SI621_001166"
                     /pseudo
     CDS             19070..20421
                     /gene="creD"
                     /locus_tag="SI621_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
     gene            complement(20481..21197)
                     /gene="arcA"
                     /locus_tag="SI621_001167"
     CDS             complement(20481..21197)
                     /gene="arcA"
                     /locus_tag="SI621_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HEI4063907.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            21293..21433
                     /locus_tag="SI621_001168"
     CDS             21293..21433
                     /locus_tag="SI621_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063908.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            21833..22519
                     /locus_tag="SI621_001169"
     CDS             21833..22519
                     /locus_tag="SI621_001169"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEI4063909.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            22733..22798
                     /gene="thrL"
                     /locus_tag="SI621_001170"
     CDS             22733..22798
                     /gene="thrL"
                     /locus_tag="SI621_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HEI4063910.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    22734..22853
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            22879..25341
                     /gene="thrA"
                     /locus_tag="SI621_001171"
     CDS             22879..25341
                     /gene="thrA"
                     /locus_tag="SI621_001171"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEI4063911.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            25343..26275
                     /gene="thrB"
                     /locus_tag="SI621_001172"
     CDS             25343..26275
                     /gene="thrB"
                     /locus_tag="SI621_001172"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEI4063912.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            26276..27562
                     /gene="thrC"
                     /locus_tag="SI621_001173"
     CDS             26276..27562
                     /gene="thrC"
                     /locus_tag="SI621_001173"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEI4063913.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            27776..28072
                     /locus_tag="SI621_001174"
     CDS             27776..28072
                     /locus_tag="SI621_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HEI4063914.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(28112..28888)
                     /gene="yaaA"
                     /locus_tag="SI621_001175"
     CDS             complement(28112..28888)
                     /gene="yaaA"
                     /locus_tag="SI621_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEI4063915.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(28958..30388)
                     /locus_tag="SI621_001176"
     CDS             complement(28958..30388)
                     /locus_tag="SI621_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEI4063916.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMALIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            30667..31620
                     /gene="tal"
                     /locus_tag="SI621_001177"
     CDS             30667..31620
                     /gene="tal"
                     /locus_tag="SI621_001177"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEI4063917.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            31735..32322
                     /gene="mog"
                     /locus_tag="SI621_001178"
     CDS             31735..32322
                     /gene="mog"
                     /locus_tag="SI621_001178"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEI4063918.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(32357..32923)
                     /gene="satP"
                     /locus_tag="SI621_001179"
     CDS             complement(32357..32923)
                     /gene="satP"
                     /locus_tag="SI621_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEI4063919.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(33072..33785)
                     /gene="msyB"
                     /locus_tag="SI621_001180"
     CDS             complement(33072..33785)
                     /gene="msyB"
                     /locus_tag="SI621_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HEI4063920.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(33811..34215)
                     /locus_tag="SI621_001181"
     CDS             complement(33811..34215)
                     /locus_tag="SI621_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HEI4063921.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKVARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            34591..36507
                     /gene="dnaK"
                     /locus_tag="SI621_001182"
     CDS             34591..36507
                     /gene="dnaK"
                     /locus_tag="SI621_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEI4063922.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            36596..37726
                     /gene="dnaJ"
                     /locus_tag="SI621_001183"
     CDS             36596..37726
                     /gene="dnaJ"
                     /locus_tag="SI621_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEI4063923.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(37830..37982)
                     /gene="mokC"
                     /locus_tag="SI621_001184"
     CDS             complement(37830..37982)
                     /gene="mokC"
                     /locus_tag="SI621_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HEI4063924.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         9190 a         9628 c        10382 g         9004 t
ORIGIN      
        1 tagagaatta atggagatac ctaacatttt acttacttca gcaagtgata gtttatgcct
       61 gatacgcacg gcagttatcc aggatatacc ctggctgaac attaaattga taatctcccg
      121 ttcctggttt tccagatcga tcatatttcc ttccctggat attttaatca gcgagttacc
      181 ttaccgcccc agccatccat gccgataact cccgtagctc tttttcctgt tccgggaagt
      241 caaccaacag cgcttcgcac tcctgttgca gcatatctgc gcggtacagg cagccttgca
      301 gtcgcccggc gagggcttcc agcggcgcgg ggttgaggct gtcggtaaac acctgggcgc
      361 gggtgatatg gcctttttca acgtcgaaat gcagttccac accgccccag gtaaagcgtt
      421 catccagcag atgcgagaat gccggagcct gaccgaagtt ccattcccag ctactctggc
      481 gggcaaaggt ttcggcgaag ttcggcaggt ctggcgtttt gtccggggag atgatttccg
      541 cttccacgcg ctcgccataa tgggcgaaaa aggcctcggt tatggcctcg caaacctgct
      601 catgggtaat ttccggcagc aattcggtga ggttggtcac gcgggaacgt accgacgtaa
      661 tgcctttcgc cgccagtttc tttttatccg gattgagata gtttgccagg cggctaaggt
      721 cggcattgag cagcaaggtg ccgtggtgga agccacgatc tttggtttcg cgataggccg
      781 agcctgagac tttgcggtcg ccttcggcgg ttttcaccac cagatcgtta cgtccggacg
      841 cttcggcgct gacgccgagc gcgttcagtg cattgagcac aatcgacgtg gagatggttt
      901 tatcgtactc cggcttgcca gccataaagg taaaacaggt attgccgaga tcgtggaaca
      961 ccgcgccgcc accgctactg cgccgcgcca ggcggacgtt atcttcttcc atccgccggg
     1021 tattacactc tttccacggg ttctgtgcgc gaccaatcac taccgtgtcg gcattgcgcc
     1081 agagaaacag aacgcgctgc gtggcgggca tctggcgaaa aatacactcc tccaccgcca
     1141 ggttaaacca cgggtcgtaa gagtcagaga tgagcaggcg taatgtggac ataacgattt
     1201 cctttcttgt agtaatgggt cactcttttt tctcgctttc ttcctcttcc ggcaccggtt
     1261 tgctggcggt taacaggaag ggcgattgct gccagcgggt gcgtttaccc tgtagcagcg
     1321 tgcgggtcag tactacgccg attgccagtg agagcagcaa catcaggcgt aaaatgttag
     1381 tggtgttatc cacctgttgg gcttcggtgg cgagcgtatg ggtgtcgagc gtcaggcgca
     1441 gatagccgag cggtccgttt ttcccggcaa taggttcgac aatctgctgg ttaaaatagc
     1501 cgcccgcttt tttgccgtcg agcgccaacc ggtcgcgcac ttcgacgctt tcgccagaac
     1561 gggcgataag atcgccttgt tcgtcataca cgcccgcgtc gaggatgcgg ctttcatccg
     1621 ttaactgatc gaggatcgcc tgaatgcgtt tttcatccgg tgagtcgtta cgcatcagcg
     1681 gtgcaacgtt cagcgtcacc tgacgcgcca gggtgcgggc cagttcttcc agctgtggat
     1741 tacgctgtcg ctggtggttt tgactaaacc atgacgctcc ctgcatcagc gccactaaca
     1801 aggcaagaca gaacaggaca atcactgccc gatgcagccg gaatttcagt tttgtgcgag
     1861 ccatcttcca ccctttgaaa atttgagact taatgttgcc agaagcaatg gatacaaggt
     1921 agcctcatgc gttattttcc ctgcttcgaa cgattttaca ggagccttaa tgcctaacat
     1981 tacctggtgc gacctgcctg aagatgtctc tttatggccg ggtctgcctc tttcattaag
     2041 tggtgatgaa gtgatgccac tggattacca cgcaggtcgt agcggctggc tgctgtatgg
     2101 tcgtgggctg gataaacagc gtctgaccca ataccagagc aaactgggcg cggcgatggt
     2161 gattgttgcc gcctggtgcg tggaagatta tcaggtgatt cgtctggcag gttcactcac
     2221 cgcgcgggct acgcgcctgg cccacgaagc gcagctggat gtcgcgccgc tggggaaaat
     2281 cccgcacctg cgcacgccgg gtttgctggt gatggacatg gattccaccg ccatccagat
     2341 tgaatgtatt gatgaaattg ccaaactggc cggaacgggc gagatggtgg cggaggtaac
     2401 cgaacgggcg atgcgcggcg aactcgattt taccgccagc ctgcgcagcc gcgtggcgac
     2461 gctgaaaggc gctgatgcca atattctgca acaggtgcgt gaaaatctgc cgctgatgcc
     2521 aggcttaacg caactggtgc tcaagctgga aacgctgggc tggaaagtgg cgattgcttc
     2581 cggcggcttt actttctttg ctgaatatct gcgcgacaag ctgcgcctga cagccgtggt
     2641 agccaatgaa ctggagatca tggatggtaa atttaccggc aatgtgatcg gcgacatcgt
     2701 tgacgcgcag tacaaagcga aaactctgac tcgcctcgcg caggagtatg aaatcccgct
     2761 ggcgcagacc gtggcgattg gtgatggagc caatgacctg ccgatgatca aagcggcagg
     2821 gctggggatt gcctaccatg ccaagccaaa agtgaatgaa aagacggaag tcaccatccg
     2881 tcacgctgac ctgatggggg tattctgcat cctctctggc agcctgaatc agaagtaatt
     2941 gctcgcccgt catcctgcgg gcggcacagc attaacgagg tacaccgtgg caaaagctcc
     3001 aaaacgcgcc tttgtttgta atgaatgcgg ggccgattat ccgcgctggc aggggcagtg
     3061 cagcgcctgt catgcctgga acaccatcac cgaggtgcgt cttgcagcgt cgccaacggt
     3121 ggcgcgtaac gagcgtctca gtggctatgc cggtagcgcc ggggtggcaa aagtccagaa
     3181 actctccgat atcagccttg aagagctgcc gcgtttttcc accggattta aagagttcga
     3241 ccgcgtacta ggcggtggcg tggtgccggg aagtgccatt ctgattggcg gtaacccagg
     3301 tgccgggaaa tccacattgc tattgcaaac gctgtgcaaa ctggcccagc agatgaaaac
     3361 gctgtatgtc actggcgaag agtcgctgca acaggtggca atgcgcgctc atcgccttgg
     3421 cctgccgacc gacaatctca atatgttgtc ggaaaccagc atcgagcaga tctgcctgat
     3481 tgccgaagaa gagcaaccga agctgatggt aattgactcc atccaggtga tgcatatggc
     3541 ggatgttcag tcatcgcctg gcagtgtggc gcaggtgcgt gaaacggcgg cttatctgac
     3601 gcgcttcgcc aaaacgcgcg gcgtggcgat tgtcatggtt gggcacgtaa ccaaagatgg
     3661 ctcgctggct ggccctaaag tgctggaaca ctgtatcgac tgttcggtgc ttctggatgg
     3721 cgatgccgac tcccgttttc gcaccttacg cagccataaa aaccgcttcg gcgcggtgaa
     3781 tgagctgggc gtcttcgcga tgaccgaaca ggggctgcgt gaagtcagca acccttctgc
     3841 catcttttta agccgcggtg atgaagtgac ctccggtagc tcagtgatgg tagtgtggga
     3901 aggaacgcgt ccgctgctgg tggagatcca ggcgctggtc gatcactcga tgatggcgaa
     3961 tccacgccgc gtcgcagtag ggctggagca aaaccgtctg gcaatcctgc tggcggtgct
     4021 gcaccgtcac ggtgggttgc aaatggccga tcaggatgta tttgttaacg tggtcggcgg
     4081 cgtgaaggtg acggaaacca gcgccgactt agcgttactg ctggcgatgg tttccagcct
     4141 gcgcgacaga ccgctgccac aggatctggt ggtgtttggt gaagtcgggc tggcagggga
     4201 gatccgcccg gtgcccagcg gccaggaacg aatctctgaa gcggcgaaac acggttttcg
     4261 ccgggcgatt gttccggcgg ctaatgtgcc gaaaaaagcg ccggaaggga tgcagatttt
     4321 tggcgttaaa aaactctccg acgcgcttag cgtgttcgac gacttataat gagagatacg
     4381 gagggagata tgtcgtcatt tgattacctg aaaactgcca tcaagcaaca gggctgcacg
     4441 ctacagcagg tagctgatgc cagcggtatg accaaagggt atttaagcca gttactgaat
     4501 gccaaaatta aaagccccag cgcgcaaaag ctggaggcgt tgcaccgttt tttggggctt
     4561 gaatttcccc ggcagaagaa aaccattggt gtcgtattcg gtaagttcta cccgctgcat
     4621 accggacata tctaccttat ccagcgcgcc tgtagccagg ttgacgagct gcatatcatt
     4681 atgggttttg acgatacccg cgaccgcgcg ttgtttgaag acagcgccat gtcgcagcag
     4741 cccaccgtgc cggatcgtct gcgctggtta ttgcaaacct ttaaatatca gaaaaatatc
     4801 cgtattcatg ctttcaacga agagggcatg gagccatatc cgcacggctg ggatgtgtgg
     4861 agcaacggca tcaaaaagtt tatggctgaa aaggggatcc agccggacct gatctacacc
     4921 tcggaagaag ccgatgcgcc acagtatatg gaacatctgg ggatcgagac ggtgctggtc
     4981 gatccgaagc gtacctttat gagtatcagc ggtgcgcaga tccgcgaaaa cccgttccgc
     5041 tactgggaat atattcccac cgaagtgaag ccgttctttg tgcgtaccgt ggcgatcctt
     5101 ggcggcgagt cgagcggtaa atccaccctg gtaaacaaac ttgccaatat cttcaatacc
     5161 accagtgcgt gggaatatgg tcgcgattat gtcttttcac acctcggcgg tgatgagatc
     5221 gcattgcagt attctgacta cgataaaatc gcgctgggac acgctcaata tattgatttt
     5281 gcagtgaaat atgccaataa agtggcgttt attgacactg attttgtcac tacccaggcg
     5341 ttctgcaaaa agtacgaagg gcgggagcat ccgttcgtgc aggcgctgat cgatgaatac
     5401 cgttttgatc tggttatctt gctggagaac aacaccccgt gggtggcgga tggtttacgc
     5461 agcctcggta gttcggtgga tcgcaaagag ttccagaact tgctggtgga gatgctggaa
     5521 gagaacaata tcgaattcgt gcgggttgaa gaggacgatt atgacagccg tttcctgcgc
     5581 tgcgtggagc tagtgcggga gatgatgggg gagcagagat aaccgtgacg aaacggctca
     5641 aaggcgaggt ataaaataag ttttttgatg agttgaaaac ctctctggaa gagactgtcg
     5701 agattagaaa gaacctgtac gggtgacccg ctacgaaatt gcggatgcta aggctattcg
     5761 tgagcaactt catactccaa ccgaccggtt gcgtagccag aatgcaatca aatctgacag
     5821 tctgcttcgg cgcgcgcttt ggtgtattta ccggagtaaa tcagcgtgcc gtggaaaatt
     5881 atgcgcagcg cttctgctga tttgcgtacc ttcgctgatg ataagtcctg cactggcgcg
     5941 ctgcgcgtaa tagtccgcca ttatgtcagt cgcaacgtca cccgcgcggg aacgcgtcat
     6001 cggtggcatt acaatacggt ttattaatgg caacgcattc agcgcatatt tagcaaatta
     6061 cgtaatgggt ccctcgcgcg attatttata cagttcggca ctcatggtga cggtattgcc
     6121 catgcgagcg ctggtaattt tatattcaga ggcgttcatc tctttcgctt tctgcgcaat
     6181 aactgcttcc gcagaatcca gtgttgtacc ggttgctgtc acgctttgcg ccgatgcgtt
     6241 aaacgccgcg acagagaaaa gagaagctgt aaaaattgcg atgatcgttt tcatgtttat
     6301 attccagagt gtcttaattg tttggcgttt attaacgcga tggggagaga atacgcctgc
     6361 tgcctgataa cataaatgat acaaatgaca atagactatt ctgattttgt taataatcag
     6421 acgccggacg ttccgacgcc tgtcgatcgc atcaggcaac aaagttgtcc ttcagcgcct
     6481 tgagaccatc ccgatagatg ccggtaaaca gcgcttcggc ctcttcgttc gtcacattca
     6541 ccggggtgaa ttcaccgaac cattccacac gggaaacctg gctgtcggcg gtttcatgaa
     6601 cggtaatggt ggagaggtaa tccaccaccg ggaacggtgc ctgaataatg gaataggtat
     6661 aactgcgttg acggttatca aacgcttcca gacgttcaac cacagtgccg ccgtcagagg
     6721 ttgtcagcga acgaacgcga ccgccttctg tcactacgct tttcgggatg aacggcagcc
     6781 agtctggcag cgcatcaaag ccgcccatca attgccatac ggcgtcggca gaagccggaa
     6841 tttcgatcga tacagttgtt ttagccatga taattttctc ttaatttata gggttacata
     6901 gtcagggtgt cgacgacacc gccatcgacg cgcagggctg cccctgtggt tgccgaggaa
     6961 agcggtgacg cgagataaac cgccagattc gctacttctt ccacatccgc cgcacgctgg
     7021 atgatggagc tcggacgcga ggttttaaca aactcatcgg cctgttcacg cgcactgcgt
     7081 cccgattgtg cagcggcatc cgccagcatc tctttcaggc catcggtaaa ggttggccct
     7141 ggcagaatgg cgttgacggt cacaccggtc cccgccagtc gtttcgccag accatgagag
     7201 accgccagat tcgcagcttt ggtgacgccg tagttgatca tgtcgccagg aattgccacg
     7261 ccggattcgg acgagacaaa gataatccgc ccccagccct gttccgtcat gcctggcgca
     7321 tagtgacgcg ccagacgaac gccggagagg acgttgacgt tatagaagtg catccactca
     7381 tcatccggaa cgctaaagaa atctttatcg ttgaaaatgc ccagattatt gaccagcaca
     7441 tcagccgttg ggactgcagc gatcagcgca gccacgccgt cttcggtgct gagatcggcc
     7501 accacgccac gcagtgacgc cgtgggagtt tgtgtcgcaa tcgctgccat ggcgtcatca
     7561 acgccgcgct gtttacggcc gacgacaacc accgttgcgc ctgcttttgc cagtccggcg
     7621 gcgataccct gcccaatgcc ggaggttgag ccgctgacca ccgcgacttt acctgtcaga
     7681 tccagtttca tcgttttctc accaggttga ttaacgggtc ggcagcggag cgttttccgc
     7741 aaccagattt tgacggcgca tttctgccca gaattcagcc ggaacaggcg cattcagtgc
     7801 agccagatct tctgccatac ggccaggacg gctggagcca ggaataaccg ctgcaacggc
     7861 cgggtttgcc agcgcgaact gcagcgcagc tgctttaacg ttcacattga aacgtgtggc
     7921 aatggcgtta atttgcgcca ctttctgctg gatggccgca gaggctttct gatattcaaa
     7981 gtgctcgccg cctgccagta caccagagct gtacggaccg cccacaacaa tatccacgtt
     8041 ctgttccagt gcttctggca tcaggcgttg cagcgcacgt tcgtgatcca gcagggaata
     8101 acgccccgcc agcaggaacg catccggctt cggttcgtcc agcgccagcg tcagttcgca
     8161 aggttcgacg cggttcacac ccagtcccca ggctttaatc acaccttcgt cacgcaaacg
     8221 tgtcagtgca cgaaacgcac cggtacgggc aatgttgaac tgctccagcc agctgtcacc
     8281 gtagaagtcc tgcgccgggt cgtggatcca gacaatgtcc agacggtctg ttttcaggcg
     8341 tttcaggctg tcttcaatcg agcgcagcgt ggcgtcagca gagtagtcat tcagaatttt
     8401 gtttttcagg ccgtgttcga acaggccacc tttttcaccg aaatcccgtt ttgccggatc
     8461 ttccatttcg tccagcatga tgcgaccgac tttgctactc agaacgtatt catcacgtgg
     8521 atactgagac agcgcttcgc ccatgcggat ttcagaaagg ccagagccgt acagcggcgc
     8581 ggtatcaaag taacgcacgc ccaggttcca ggcttcgctc acggtggcgt gggcctcttc
     8641 ttccggaatg gcacggaaca tgttgccgag tggtgcgccg cctaagccaa ttttgccggg
     8701 aagtaaattt ttgatgctca taagatgtcc tcaattattc ggtttgggtt agggcgtttt
     8761 tttgctatcg ccctgtgacg tggatcacaa taccttgcga ggttgtgact tgataggaca
     8821 taattcgtct gacttaagtc ctttgaggtc taacatgatt gatttacgcc aactgaagta
     8881 cttcgtggtg gtcgcagaag aagagcacgt gggacgcgca gccgagcgat tacatatctc
     8941 gcaatcaccg ctgagccgcc agattgcaca actggaagaa cggctggggc tgacgctgtt
     9001 tgagcgtaat cagcagcgca ttcgcctgac cgctgatgga cagacattcc ttgccgaaac
     9061 acgttcgttg ctgacacacg ccaaccgcct ggagtcgtta ggcaaacgcc tgggacgggg
     9121 ggaagatggg ggcttgtgta ttggctatat cgaaaacgcc atgcattccg gtgtactttc
     9181 tacggcgtta cgtacgttac gtgtatcgcg tccgaatgtg catatcgctc tttacagcca
     9241 accaccggcg acccagattg aaggcctgcg ccagcgcagt ctcgattttt ctctggtcga
     9301 tacgccgccc accgacagcg agcaggatct ggaatatatt caggttctgg atgatcgaat
     9361 gttgttggcc ttaccgcaaa atcatcctct ggcgcaggag gaaaaccttg tcgcgtcaca
     9421 acttggcgat gagcagtgga ttgcggtgat tcataacgac gatgcgcatt ctcgcgatca
     9481 ttttgtcgcc gcctgtgtta acgccgggtt taatcctgat attcgcctgg aagcgggtga
     9541 acccctcacg gcattaggtt tggtcgctgc gggattaggt ctggccttaa ttcaacatag
     9601 tctgcgtcat aatgcgccgg aaggggtggt attacgtgaa ttgccgtgga tgaattattc
     9661 aacgcaatta tgggcagcct ggcataaagc caaccaacgt ccgctggttt cccatttccg
     9721 ccagattttg cgcggtgaat aaaacgttaa gcgggaacca tgagttcccg cttaatttga
     9781 gatgtcagtc cgcatctgag ctaatacgct taaagaaaat tctgcctttt ccggggacta
     9841 atcatttaac taataccgga tgcgatgctg gcgcgtcgca tccggcattt tacgcattac
     9901 ttcgcaatac gcttgtactt gatacgcttc ggctccagcg cgtctgcgcc cagcgtgcgt
     9961 ttcttgtact cttcgtactc ggtaaagtta ccttcgaaga actcaacttt accttcatcc
    10021 tggtagtcca ggatgtgcgt ggcgatacgg tcgaggaacc aacggtcgtg cgagataacc
    10081 atcgcgcagc ccgggaactc cagcagggcg ttttccagcg cacgcagggt ttcgatatcc
    10141 aggtcgttgg tcggttcgtc gagcagcagc atgttgccgc caacttgcag cagcttcgcc
    10201 agatgcagac gaccgcgctc accgccggag agttcaccaa cgcgtttacc ctgatcaacc
    10261 cctttaaagt taaagcggcc aacgtaggca cggcttggca tctcggtgtt gccgatcttc
    10321 atgatatcca gcccgccgga aacttcttcc caaacggttt tgctgttatc cattgagtca
    10381 cggaactgat caaccgatgc tagtttcacc gtttcaccta aagtgatggt gccgctgtcc
    10441 ggctgttcct gaccggagat catacggaac agggtcgatt tacccgcacc gttcggaccg
    10501 atgatcccga cgatcgctcc tttcgggatc gagaagctca ggtcatcaat cagcagacga
    10561 tcgccatagg atttacgcag gttgttgact tccagcactt tatcgccaag acgcggtccc
    10621 ggtggaataa acagttcgtt ggtttcgtta cgtttctgat attcggtgct gttcagctct
    10681 tcaaaacgcg ccagacgtgc tttacctttc gactgacggc ctttagtccc ctgacgtacc
    10741 cactccagct ctttctcaat cgacttacga cgcgcagctt cttgtgaagc ttcctgcgcc
    10801 agacgttgat ctttctgctc cagccaggag gagtagttac cttcccacgg aataccttca
    10861 ccacggtcca gttcgaggat ccagcccgcc acgttatcga ggaagtaacg gtcgtgggta
    10921 atcgccacaa cagtgccttc gaagtcgtgc aggaagcgtt ccagccacgc cacggattcg
    10981 gcatccaggt ggttcgtcgg ttcgtcgagc agcagcatgt ctggtttttc cagcagcagg
    11041 cggcacaacg ccacgcgacg acgctcacca ccggagaggt tagcgatttt cgcgtcccag
    11101 tccggcagac gcagcgcatc cgccgcacgc tccagctgaa cgttcaggtt gtgaccgtcg
    11161 tgagcctgaa tgatctcttc cagacggcct tgttcagcgg ccagcttgtc aaaatcggca
    11221 tccggatcgg cgtacagcgc atacacttca tccaggcgtt tcagggcgtt aaccacttca
    11281 gaaaccgctt cttcaatgga ctcacgcacg gtgtgttccg ggttcagctg cggttcctgc
    11341 ggcagataac caatcttgat atccggttgc ggacgcgctt cgccttcgat gtctttatca
    11401 atgcccgcca taatgcgcag cagggtggac ttacccgcgc cgttcagacc caggacacca
    11461 atttttgccc cagggaagaa actcagagag atgtttttca aaatatgacg tttcggcgga
    11521 acaactttgc cgacacgatg catggtataa acgaattgag ccacgttgga cttcgcctct
    11581 atgtttatcg tgatgatgag ttttcaaagg cgaagtgtag cctttttccc cgcctaatcc
    11641 cagccggtcg atcaccctcg cagtaaaagt aaaaaagtgt ccgtaacgtg gcgtaaacgg
    11701 caatgactgg ttagcataaa tctattacgc ggcatgacgc tgcattgatg tatttacact
    11761 tagaggatgc gcttgtggaa aaagccaaac aagttacctg gcggctgttg gctgccggtg
    11821 tctgtctgct gacggtcagc agcgtggcgc gagccgactc actggatgag cagcgtagcc
    11881 gctacgccca aatcaagcag gcctgggata atcgacaaat ggatgtggtc gaacaaatga
    11941 tgcctggact gaaggattat ccgctttatc cctacctgga ataccgtcag atcaccgatg
    12001 acctgatgaa tcaaccggcg gtgacggtca ctaactttgt tcgcgctaac cccacgcttc
    12061 ctcccgctcg cacgctgcaa tctcgtttcg tcaatgaact ggcgcggcgt gaagactggc
    12121 gtggcttgtt agcctttagc ccggaaaagc ccggaactac cgaagcgcaa tgtaattact
    12181 actatgcgaa atggaatacc gggcagagtg aagaagcctg gcaaggggcg aaagagctgt
    12241 ggctaaccgg caagagccag cctaacgcct gtgacaagtt atttagcgtc tggcgtgcgt
    12301 caggtaaaca agatccgctg gcgtatttag agcgtatccg tttggcgatg aaagcgggta
    12361 acactggact ggtaacagtg ctggcagggc agatgcctgc cgattaccag actatcgcct
    12421 cggcaatcat ttcactggcg aacaacccta atacggtact gaccttcgcg cgtacaaccg
    12481 gagcgaccga ttttacccgt caaatggcgg cggtggcgtt tgccagtgtg gcgcggcagg
    12541 atgctgagaa tgcacggctg atgatcccat cgcttgccca ggcgcagcag cttaatgaag
    12601 atcagattca ggagctgcgc gatatcgtcg cctggcgttt gatgggcaac gatgtcaccg
    12661 acgagcaggc gaaatggcgc gatgacgcca ttatgcgctc gcaatccacg tcgttaattg
    12721 aacgccgtgt gcgaatggcg cttggcaccg gcgatcgtcg cggcctgaat acctggctgg
    12781 cgcgtttgcc gatggaggcg aaagagaaag atgaatggcg ttactggcag gcggatttac
    12841 tgctggaacg cggacgtgaa gctgaagcaa aagagatttt gcatcaactc atgcaacagc
    12901 gtggtttcta cccgatggtt gcagcacaac gcatcggaga agagtatgag ctgaagattg
    12961 ataaagcgcc gcagaatgtt gacagcgccc tgactcaggg gccggagatg gcgcgcgtgc
    13021 gcgagttgat gtactggaat ctcgataaca ccgcgcgtag cgagtgggcc aatctggtga
    13081 agagcaagtc aaaaacagag caggcgcaac tggctcggta tgctttcaat aaccaatggt
    13141 gggatcttag cgttcaggca acgatcgccg ggaagctttg ggatcatctg gaagaacgat
    13201 tcccgctggc ctacaacgat cttttcaaac gttacaccag tgggaaggag atcccgcaaa
    13261 gctatgcgat ggcgattgcc cgtcaggaga gtgcctggaa tccgaaagtg aaatcgccgg
    13321 taggggccag cggcctgatg cagattatgc cgggcacggc gacccatacg gtgaagatgt
    13381 tctctattcc tggttatagc agcccaggac aattgctgga tccggaaacg aacatcaaca
    13441 ttggcaccag ttacctgcaa tatgtttatc agcagtttgg taataaccgt attttctcct
    13501 cagcagctta taacgccgga ccggggcggg tgcgaacctg gcttggcaac agcgccgggc
    13561 gtatcgacgc agtggcattt gtcgagagta ttccgttctc cgagacgcgc ggttatgtga
    13621 agaacgtgct ggcttatgac gcttactacc gctatttcat gggggataaa ccgacattga
    13681 tgagcgccac ggaatgggga cgtcgttact gatccgcacg tttatgatat gctatcgtac
    13741 tctttagcga gtacaaccgg gggaggcatt ttgcttcccc cgctaacaat ggcgacatat
    13801 tatggcccaa caatcaccct attcagcagc gatggcagaa cagcgtcacc aggagtggtt
    13861 acgttttgtc gacctgctta agaatgccta ccaaaacgat ctccatttac cgttgttaaa
    13921 cctgatgctg acgccagatg agcgcgaagc gttggggact cgcgtgcgta ttgtcgaaga
    13981 gttgttgcgc ggcgaaatga gccagcgtga gttaaaaaat gaactcggcg cgggcatcgc
    14041 gacgattacg cgtggatcta acagcctgaa agccgcgccc gtcgagctgc gccagtggct
    14101 ggaagaggtg ttgctgaaag atcactgata caccgcatta tgaaacggac tcagcgccag
    14161 gatcaccgcc tggtgataga cgctggcgcg ggtaagtttc ccggcggtaa acacgccgat
    14221 cgccccttcc ttacgaccaa tctcatcaat accggtatag cgcgacatca cgggaccgag
    14281 cgcctcgcct tcacgcactt tttccagaat caccgcaggt aacggcaaag tagccgaacg
    14341 cgcctcgccg cgctggctgg cgttttcaat caccacccaa ctgaaggtgc tgtcgccatc
    14401 aatgccagct tcaatcgcca cccaaaaatc agcctctgga tgtaaacggc gggcatttgc
    14461 tacccgattt cgtgcgccag cgcgcgtttc ctcactgcca aagggctgtt ccggtacacc
    14521 gctctcgacg gcaacggatg caatatggca ggatccttcg ccgaagatct cgtgaaatgc
    14581 ctgcagaatg gcctgaattt tagcgggatt ggtggtcgca cagacaactt ggtgcataat
    14641 cagcattact cagaaaatta acgttacagc agtatacgga aaaaaagcat gttacaggta
    14701 tacctagtcc gccacggtga aacgcagtgg aacgccgagc gacgtattca gggccagtct
    14761 gacagcccgc tgaccgccaa aggtgagcaa caggcgatgc aggtggcaac ccgtgccaaa
    14821 gagcttggca ttacgcatat catcagtagc gatttaggac gcacccggcg tacggcggaa
    14881 atcatcgccc aggcctgcgg ctgtgacatc atctttgatt ctcgcctgcg tgaattaaac
    14941 atgggtgtgc tggaaaaaag acatatcgat tctctgaccg aagaagaaga gaactggcgt
    15001 cggcagctgg tcaatggcac cgttgacggg cgtattcctg aaggcgagtc aatgcaggag
    15061 ctcagcgatc gcgtcaatgc ggcactggaa tcttgccggg acttaccgca gggaagccga
    15121 ccgctgctgg tgagccacgg tattgcactg ggatgcctgg tgagtacgat tctcggatta
    15181 ccagcatggg cagagcgtcg cttacgtctg cgtaactgtt ctatttcgcg cgtggattat
    15241 caggaaagcc tgtggctggc gtctggttgg gtcgttgaaa ccgcagggga catctcgcat
    15301 ctggacgccc ctgcattaga tgagctgcag cgttaacgac ggatcggaat cagcagttca
    15361 cagcgcagat taattgggcg atctcccgct ttggcatctt ctgccgggta gtatcgctca
    15421 atatcctgac ctttacggcg cgtcaggttg agcattggca tacacgttcc gtataccgtc
    15481 aggataaact cctgcacgcc ggttcccaga ccttcatagg taaacatcac atattcgccg
    15541 ccctgcagca tcaccggatg ccccgtcagt acatagccat ctgcctgatc ctgggctaac
    15601 gcggtggtat agaatacttc ttgttcgtcg tctttatcct gactcggacg cgtttcattc
    15661 aggccgtaga gcaccggcgg aatggtcggc gcgttgccga gaaaatcgtg ccagaactga
    15721 taacgcattt catggcggaa atcagagatt tgctccagcg aacaggagta gctctgggta
    15781 acaccaatca gcggcgtatc ttccagggtg acaaatttgt gctctggcat agtgaattca
    15841 cccaggcgta gcggcgggcg aataccaaag gcgctccatt caggagaacg gcggtaaagt
    15901 gcaggagtct gggcaaactg cttcttgaat gcgcgggtaa atgtctgttg agagtcgaag
    15961 cggtattgca gcgcgatgtc cagaatcgga cgcgcagtca ggcgtagtgc gaccgccgat
    16021 ttcgacaaac gacgagcacg aatatacgcg ccaatagcat ggccagtgac atctttaaac
    16081 attctctgta agtgccactt ggaataacct gctttcgccg ctacattgtc gagcgacagg
    16141 ggctgatcca gatgaccttc cagccagatt aaaaggtcgc gaataatgcc ggcctgatcc
    16201 ataaaatatc ctcatccttt caacaacgag cacctgacat caggtaattg gataatagca
    16261 ttttttgctg ttttagcatt cagtgttttt ttcttagtag agtatgtttt aggacttgac
    16321 ggaaataaaa gtattgagat tttgttctta atcaatatgt tatttaccgt gacgaactaa
    16381 ttgctcgtgt aatagataaa aatggtaaca atatgaaata caagcatttg atcctgtctt
    16441 taagcctgat aatgctgggg ccattggctc atgcagaaga gattggttcg gtcgacaccg
    16501 tatttaaaat gatcggcccg gatcacaaaa ttgttgtgga agcctttgac gatcccgatg
    16561 tgaaaaatgt cacctgttat gtgagccggg cgaaaaccgg tggtattaaa gggggattgg
    16621 gcctggcaga agatacctct gatgcggcca tttcttgtca gcaagtcggg ccgattgaac
    16681 tgtcggatcg tattaaaaac ggcaaagctc agggcgaggt agtattcaaa aaacgcacgt
    16741 ccctggtctt taagtcgtta caggtcgtgc gcttttatga tgccaaacgc aacgcgctcg
    16801 cttatctggc ttactccgac aaagttgtag aaggttcgcc gaaaaacgcg attagcgcgg
    16861 ttcctgtcat gccgtggcgg caataacaga ggcgatttat gcaacgggaa acggtctggt
    16921 tagtggaaga tgagcaaggg atagccgaca cgctggtcta catgttgcag caggaaggtt
    16981 ttgccgtcga ggtctttgag cgaggcttgc cggtgctgga taaagctcgc cagcaggtac
    17041 ccgacgtcat gattctcgat gttggtctgc cggatattag cggctttgaa ttgtgccgcc
    17101 agttactggc gctccatccg gcgttacctg tactgttcct gacggcccga agtgaagagg
    17161 tcgatcgcct gcttgggctg gaaattggcg ctgacgacta tgtggctaaa ccgttttcac
    17221 cccgcgaagt gtgcgccagg gtgcgcacct tactgcgtcg ggtgaagaag ttctcgacgc
    17281 cgtctcccgt catccgtatt ggacattttg aattgaatga acccgcggcg cagatcagct
    17341 ggtttgacac gccattaacg ctgacgcggt atgagttttt attgctgaag acgttactca
    17401 agtcgccagg ccgcgtctgg tcccgccagc aactgatgga tagcgtgtgg gaagatgcgc
    17461 aggacaccta cgatcgcacc gtcgataccc acattaaaac gttgcgtgcc aagctgcgcg
    17521 ccatcaaccc cgatctttca ccgattaata ctcatcgcgg catgggatat agcctgaggg
    17581 gcctgtaatg cgtatcggca tgcggttgct gctgggctat tttttactgg tggcggtggc
    17641 agcctggttc gtactggcca tttttgtcaa agaagttaaa ccgggcgtgc gaagagcaac
    17701 ggaggggaca ttgatcgaca ccgcaacgtt gctggcggag ctggcgcgtc ccgatttgct
    17761 ctctggggac ccaacgcatg ggcaactggc gcaggcgttt aatcagctac agcatcgccc
    17821 gtttcgcgcc aatatcggtg gcattaacaa agtgcgcaat gaatatcatg tctatatgac
    17881 cgatgcgcag ggcaaagtat tgttcgattc ggcaaataaa gccgttggac aggattattc
    17941 gcgctggaat gacgtctggc taacgttgcg tggtcagtat ggtgcgcgca gcacgttgca
    18001 aaatcctgcc gatcccgaaa gttcggtgat gtatgttgcc gcgccgatta tggacggctc
    18061 gcggcttatt ggcgttttga gcgtaggcaa accgaacgcg gcgatggctc cggtcattaa
    18121 acgcagcgag cggcgaattt tatgggccag cgccattttg ttggggattg cactggtgat
    18181 tggcgcaggc atggtttggt ggatcaaccg ctccattgcc cggctcactc gctatgctga
    18241 ttccgtcact gacaataagc ccgttcctct tcccgatctc ggtagtagcg agctgcgtaa
    18301 actcgcgcag gcgctggaaa gtatgcgcgt gaagctggaa gggaaaaact atattgagca
    18361 gtatgtttac gcattaactc atgagctaaa aagcccactg gcggcgattc gtggagcggc
    18421 ggaaatttta cgcgaaggtc cgccgccgga agtggtggct cgttttactg acaacattct
    18481 gacgcaaaat gcgcgtatgc aggcattggt agaaacgtta ctacgccagg caagactgga
    18541 gaatcgtcag gaagtcgttc tgactgctgt tgatgtggcg gcattattcc gccgcgtcag
    18601 cgaagcgcgc accgtgcagt tggcagaaaa aaaaatcact ctgcatgtta cgcccaccga
    18661 ggttaacgtt gctgctgaac cggcgttact ggagcaggcg ctgggaaatt tactggataa
    18721 cgccatcgat tttacccccg agagcggttg catcacgcta agcgccgaag tggatcagga
    18781 acacgtcacg cttaaggtgc tggataccgg tagtggtatt cctgactacg cgctttcacg
    18841 tatttttgaa cgcttttact ctttgcctcg tgcaaatggt caaaaaagca gcggtctggg
    18901 gttggcgttc gtcagtgagg tcgcccgttt gtttaacggc gaagtcacgc tgcgcaacgt
    18961 gcaggaaggt ggcgtgctgg cttcgcttcg acttcaccgt cacttcacat agcttcaaat
    19021 tcttcccaca tagccttcgt atcctgctgc cattgcaaag gagaagacta tgttgaaatc
    19081 ccccctgttc tggaaaatga ccaccctgtt tggtgcagta ttgctgttgt tgattccgat
    19141 aatgctgatt cggcaggtga ttgtcgaacg tgctgattac cgtagcgatg tggaagatgc
    19201 gattcgccaa agtaccagcg ggccgcaaaa actcgttggg ccgctcatcg ctattcctgt
    19261 gaccgagctt tatacggtgc aggaagagga taaaaccgtg gagcggaaac gaagttttat
    19321 ccatttttgg ttacctgagt cattgatggt tgatggcaat cagaacgtgg aagaacgcaa
    19381 gatagggatt tataccggtc aggtctggca cagtgattta acgctaaaag ccgatttcga
    19441 tgtttcgcgt cttagcgaac tcaacgcgcc aaatatcacc ttaggcaagc catttattgt
    19501 gattagcgtg gggatgcgcg tggtattggt gtggtgaaag cgcctgaagt taacggaacg
    19561 gcgctgacca ttgaaccggg caccggctta gagcaaggcg ggcagggcgt gcatatccct
    19621 ttacccgaag gggactggcg caagcagaac ctgaagctga atatggcact gaatttaagc
    19681 ggtaccggcg atctttctgt ggtgcctgcc gggcgtaata gcgaaatgac cttaaccagc
    19741 aactggccgc atcccagttt tttaggtgat tttctaccag ccaaacggga agttagcgag
    19801 tcaggttttc aggcgcaatg gcaaagcagc tggtttgcta ataatctcgg tgagcgtttt
    19861 gcttcaggca atgataccgg ctgggaaaac ttccccgcgt ttagcgtcgc agtgacgacg
    19921 ccagccgatc aataccaatt aacggaccgg gcgactaagt acgccattct gctgattgca
    19981 ctgactttta tggcgttctt tgtttttgaa acgctcaccg cgcaacgttt acacccaatg
    20041 caatatttgc tggtggggct ttcattggtg atgttttatt tgctcttgct ggcgctttct
    20101 gaacatatcg gttttaccgt ggcatggata atagccagtc tgattggggc gttaatgaac
    20161 ggtatttatt tgcaggcggt attgaaaggc tggcgcaata gcatgttgtt taccctcgcg
    20221 ctgttgttgc tggatggtgt gatgtgggga ctgctcaact ctgccgatag cgcgctgttg
    20281 ttgggaacca gtgtgctggt ggtggcgctg gccggcatga tgtttgtgac ccgtaatatc
    20341 gactggtatg cgttttcact gccgaaaatg aaagccagta aagaagttac aacggacgat
    20401 gaattacgta tctggaaata aggttgaaaa ataaaaacgg cgctaaaaag cgccgttttt
    20461 tttgacggtg gtaaagccga ttaatcttcc agatcaccgc agaagcgata accttcaccg
    20521 tgaatggtgg cgatgatttc cggcgtatcc ggcgtagatt cgaaatgttt acgaatacgg
    20581 cggatcgtca cgtctacagt acggtcgtgc ggtttcagct cacggccggt cattttcttc
    20641 agcagttcag cacgggactg aattttgcct gggttttcac agaagtgaag catggcgcgg
    20701 aactcgctgc gcggcagctt gtactgctcg ccatcagggc cgatcaacga acggctgttg
    20761 atgtccagtt cccaaccatt gaacttgtag ctttcaacgc tacgacgttc ttcgctgaca
    20821 gtacccagat tcatggtacg ggacagcagg ttgcgtgcac gaatagtcag ttcacgcggg
    20881 ttgaacggtt tggtgatgta gtcatctgca ccgatttcga ggccgagaat tttatcgact
    20941 tcgttgtcac ggccagtcag gaacatcaac gcaacattcg cctgctcgcg cagttcacgc
    21001 gctaacagaa gaccgttctt acccggcaga ttgatatcca tgatcaccag gttgatgtca
    21061 tattcagaga ggatctgatg catttccgcg ccatctgtcg cttcgaaaac atcatagcct
    21121 tccgcttcga aaatactttt caacgtgttg cgtgttacca actcgtcttc aacgataaga
    21181 atgtgcgggg tctgcatgtt tgctacctaa attgccaact aaatcgaaac aggaagtaca
    21241 aaagtccctg acctgcctga tgcatgctgc aaattaacat gatcggcgta acatgactaa
    21301 agtacgtaat tgcgttcttg atgcactttc catcaacgtc aacaacatca ttagcttggt
    21361 cgtgggtact ttccctcagg acccgacagt gtcaaaaacg gctgtcatcc taaccatttt
    21421 aacagcaaca taacaggcta agaggggccg gacacccaat aaaactacgc ttcgttgaca
    21481 tatatcaagt tcaattgtag cacgttaaca gtttgatgaa atcatcgtat ctaaatgcta
    21541 gctttcgtca cattatttta ataatccaac tagttgcatc atacaactaa taaacgtggt
    21601 gaatccaatt gtcgagattt attttttata aaattatcct aagtaaacag aaggatatgt
    21661 agcatttttt aacaattcaa ccgttagtac agtcaggaaa tagtttagcc ttttttaagc
    21721 taagtaaagg gctttttctg cgacttacgt taagagtttg taaattcgca ccgcgtaata
    21781 agttgacagt gatcactcgg ttcgcggtta tttgatcaag aagagtggca atatgcgtat
    21841 aacgattatt ctggtcgcac ccgccagagc agaaaatatt ggggcagcgg cgcgggcaat
    21901 gaaaacgatg gggtttagcg aactgcggat tgtcgatagt caggcgcacc tggagccagc
    21961 cacccgctgg gtcgcacatg gatctggtga tattattgat aatattaaag ttttcccgac
    22021 actggctgaa tcgttacacg atgtcgattt cactgtcgcc accactgcgc gcagtcgggc
    22081 gaaatatcat tactacgcta cgccagtaga actggtgccg ctgttagagg aaaaatcttc
    22141 atggatgagc catgccgcgc tggtgtttgg tcgcgaagat tccgggttga ctaacgaaga
    22201 gttagcgttg gctgacgttc ttactggtgt gccgatggtg gcggattatc cttcgctcaa
    22261 tctggggcag gcggtgatgg tctattgcta tcaattagca acattaatac aacaaccggc
    22321 gaaaagtgac acaacggcag acaaacatca actgcaagct ttacgcgaac gagccatggc
    22381 actgctgacg actctggcag tggcagatga cataaaactg gtcgactggt tacaacaacg
    22441 cctggggctt ttagagcaac gagacacggc aatgttgcac cgtttgctgc atgatattga
    22501 aaaaaatatc accaaataaa aaacgcctta gtaagtattt ttcagctttt cattctgact
    22561 gcaacgggca atatgtctct gtgtggatta aaaaaagagt gtctgatagc agcttctgaa
    22621 ctggttacct gccgtgagta aattaaaatt ttattgactt aggtcactaa atactttaac
    22681 caatataggc atagcgcaca gacagataaa aattacagag tacacaacat ccatgaaacg
    22741 cattagcacc accattacca ccaccatcac cattaccaca ggtaacggtg cgggctgacg
    22801 cgtacaggaa acacagaaaa aagcccgcac ctgacagtgc gggctttttt ttcgaccaaa
    22861 ggtaacgagg taacaaccat gcgagtgttg aagttcggcg gtacatcagt ggcaaatgca
    22921 gaacgttttc tgcgtgttgc cgatattctg gaaagcaatg ccaggcaggg gcaggtggcc
    22981 accgtcctct ctgcccccgc caaaatcacc aaccacctgg tggcgatgat tgaaaaaacc
    23041 attagcggcc aggatgcttt acccaatatc agcgatgccg aacgtatttt tgccgaactt
    23101 ttgacgggac tcgccgccgc ccagccgggg ttcccgctgg cgcaattgaa aactttcgtc
    23161 gatcaggaat ttgcccaaat aaaacatgtc ctgcatggca ttagtttgtt ggggcagtgc
    23221 ccggatagca tcaacgctgc gctgatttgc cgtggcgaga aaatgtcgat cgccattatg
    23281 gccggcgtgt tagaagcgcg tggtcacaac gttaccgtta tcgatccggt cgaaaaacta
    23341 ctggcagtgg ggcattacct cgaatctacc gtcgatattg ctgagtccac ccgccgtatt
    23401 gcggcaagtc gtattccggc tgatcacatg gtgctgatgg caggcttcac cgccggtaat
    23461 gaaaaaggcg aactggtggt acttggacgc aacggttccg actactccgc ggcggtgctg
    23521 gctgcctgtt tacgcgccga ttgttgcgag atttggacgg acgttgacgg ggtctatacc
    23581 tgcgacccgc gtcaggtgcc cgatgcgagg ttgttgaagt cgatgtccta ccaggaagcg
    23641 atggagcttt cctacttcgg cgctaaagtt cttcaccccc gcaccattac ccccatcgcc
    23701 cagttccaga tcccttgcct gattaaaaat accggaaatc ctcaagcacc aggtacgctc
    23761 attggtgcca gtcgtgatga agacgaatta ccggtcaagg gcatttccaa tctgaataac
    23821 atggcaatgt tcagcgtttc cggcccgggg atgaaaggaa tggtcggcat ggcggcgcgc
    23881 gtctttgccg cgatgtcacg cgcccgtatt tccgtggtgc tgattacgca atcatcttcc
    23941 gaatacagta tcagtttctg cgttccgcaa agcgactgtg tgcgggctga acgggcaatg
    24001 caggaagagt tctacctgga actgaaagaa ggcttactgg agccgctggc ggtgacggaa
    24061 cggctggcca ttatctcggt ggtaggtgat ggtatgcgca ccttgcgtgg gatctcggcg
    24121 aaattctttg ccgcgctggc ccgcgccaat atcaacattg tcgccattgc tcagggatct
    24181 tctgaacgct caatctctgt cgtggtaaat aacgatgatg cgaccactgg cgtgcgcgtt
    24241 actcatcaga tgctgttcaa taccgatcag gttatcgaag tgtttgtgat tggcgtcggt
    24301 ggcgttggcg gtgcgctgct ggagcaactg aagcgtcaac aaagctggct gaagaataaa
    24361 catatcgact tacgtgtctg cggtgttgcc aactcgaagg cactgctcac caatgtgcat
    24421 ggcctaaatc tggaaaactg gcaggaagaa ctggcgcaag ccaaagagcc gtttaatctc
    24481 gggcgcttaa ttcgcctcgt gaaagaatat catctgctga acccggtcat tgttgactgc
    24541 acctccagcc aggcagtggc ggatcaatat gccgacttct tgcgcgaagg tttccacgtt
    24601 gtcacgccga acaaaaaggc caacacctcg tcgatggatt actaccatct gttgcgtcat
    24661 gcggcggaaa aatcgcggcg taaattcctc tatgacacca acgttggggc tggattaccg
    24721 gttattgaga acctgcaaaa tctgctcaat gctggtgatg aattgatgaa gttctccggc
    24781 attctttcag gttcgctttc ttatatcttc ggcaagttag acgaaggcat gagtttctcc
    24841 gaggcgacta ctctggcgcg ggaaatgggt tataccgaac cggacccgcg agatgatctt
    24901 tctggtatgg atgtggcgcg taaactattg attctcgctc gtgaaacggg acgtgaactg
    24961 gagctggcgg atattgaaat tgaacctgtg ctgcccgctg agtttaacgc cgagggtgat
    25021 gttgccgctt ttatggcgaa tctgtcacag ctcgatgatc tctttgccgc gcgcgtggcg
    25081 aaggcccgtg atgaaggaaa agttttgcgc tatgttggca atattgatga agatggcgtc
    25141 tgccgcgtga agattgccga agtggatggt aatgatccgc tgttcaaagt gaaaaatggc
    25201 gaaaacgccc tggcctttta tagccactat tatcagccgc tgccgttggt gctgcgcgga
    25261 tatggtgcgg gcaatgacgt tacagctgcc ggtgtctttg ccgatctgct acgtaccctc
    25321 tcatggaagt taggagtctg acatggttaa agtttatgcc cctgcttcca gtgccaatat
    25381 gagcgtcggg tttgatgtgc tcggggcggc ggtgacacct gttgatggtg cattgctcgg
    25441 agatgtagtc acggttgagg cggcagagac attcagtctc aacaacctcg gacgctttgc
    25501 cgataagctg ccgtcagaac ctcgggaaaa tatcgtttat cagtgttggg agcgtttttg
    25561 ccaggagctg ggtaagcaaa ttccagtggc gatgaccctg gaaaagaata tgccgatcgg
    25621 ttcgggctta ggctccagcg cctgttcagt ggtcgcggcg ctgatggcga tgaatgaaca
    25681 ctgcggcaag ccgcttaatg acactcgttt gctggctttg atgggcgagc tggaaggccg
    25741 tatctccggc agcattcatt acgacaacgt ggcaccgtgt tttcttggtg gtatgcagtt
    25801 gatgatcgaa gaaaacgaca tcatcagcca gcaagtgcca gggtttgatg agtggctgtg
    25861 ggtgctggcg tatccgggga ttaaagtctc gacggcagaa gcccgggcta ttttaccggc
    25921 gcagtatcgc cgccaggatt gcattgcgca cgggcgacat ctggcaggct ttattcacgc
    25981 ctgctattcc cgtcagcctg agcttgccgc gaagctgatg aaagatgtta tcgctgaacc
    26041 ctaccgtgaa cggttactgc caggcttccg gcaggcgcgg caggcggtcg cggaaatcgg
    26101 cgcggtagcg agcggtatct ccggctccgg cccgactttg ttcgctctgt gtgacaagcc
    26161 ggataccgcc cagcgcgttg ccgactggtt gggtaagaac tacctgcaaa atcaggaagg
    26221 ttttgttcat atttgccggc tggatacggc gggcgcacga gtactggaaa actaaatgaa
    26281 actctacaat cttaaagatc acaatgagca ggtcagcttt gcgcaagccg taacccaggg
    26341 gttgggcaaa aatcaggggc tgtttttccc gcacgacctg ccggaattca gcctgactga
    26401 aattgatgag atgctgaagc tggattttgt cacccgcagt gcgaagatcc tctcggcgtt
    26461 tattggtgat gaaatcccgc aggaaatcct ggaagagcgc gtacgcgcgg cgtttgcctt
    26521 cccggctccg gtcgccaatg ttgaaagcga tgtcggttgt ctggagttgt ttcacgggcc
    26581 aacgcttgca tttaaagatt tcggcggtcg ctttatggca caaatgctga cccatattgc
    26641 gggcgataag ccagtgacca ttctgaccgc gacctccggt gataccggag cggcagtggc
    26701 tcatgctttc tacggtttgc cgaatgtgaa agtggttatc ctctatccac gaggcaaaat
    26761 cagtccactg caagaaaaac tgttctgtac attgggcggc aatattgaaa ctgttgccat
    26821 cgacggcgat ttcgatgcct gtcaggcgct ggtgaagcag gcgtttgatg atgaagagct
    26881 gaaagtggcg ctggggttaa actcggctaa ctcgattaac atcagccgtt tgctggcgca
    26941 gatttgctac tactttgaag ctgttgcgca gctgccgcag gaagcgcgca accagctggt
    27001 tgtctcggtg ccaagcggaa acttcggcga tttgacggcg ggtctgctgg cgaagtcact
    27061 cggtctgccg gtgaaacgtt ttattgctgc gaccaacgtg aacgataccg tgccacgttt
    27121 cctgcacgac ggtcagtggt cccccaaagc gactcaggcg acgttatcca acgcgatgga
    27181 cgtgagtcag ccgaacaact ggccgcgtgt ggaagagttg ttccgccgca aaatctggca
    27241 actgaaagag ctgggttatg cagccgtgga tgatgaaacc acgcaacaga caatgcgtga
    27301 gttaaaagaa ctgggctaca cctcggagcc gcacgctgcc gtagcttatc gtgcgctgcg
    27361 tgaccagttg aatccaggcg aatatggctt gttcctcggc accgcgcatc cggcgaaatt
    27421 taaagagagc gtggaagcga ttctcggtga aacgttggat ctgccaaaag agctggcaga
    27481 acgtgctgat ttacccttgc tttcgcataa tctgcccgcc gattttgctg cgttgcgtaa
    27541 attgatgatg aatcatcagt aatatctatt cattatctca atcaggccgg gtttgctttt
    27601 atgcagcccg gcttttttgt gaagaaaata tggagagaaa cgacagggaa aaaggagaaa
    27661 ttctcaataa atgcggtaac ttagagatta ggattgcgga gaataacaac cgccgttctc
    27721 atcgcgtaat ctccggatat cgacccataa cgggcaatga taaaaggagt aacctgtgaa
    27781 aaagatgcaa tctatcgtac tcgcactttc cctggttctg gtcgctccca tggcagcgca
    27841 ggctgcggaa attacgttag tcccgtcagt aaaattacag ataggcgatc gtgataatcg
    27901 tggctattac tgggatggcg gtcactggcg cgaccacggc tggtggaaac aacattatga
    27961 atggcgaggc aatcgctggc acccatatgg accgccgcca tcgccgcgcc ataacaagca
    28021 caatgatcat cgtggcgatc atcgtccggg gcctgacaaa catcatcgct aaatatcaat
    28081 gccggattgc catccggcat tgactacaaa cttaacgctg ctcgtagcgt ttaaacacca
    28141 gttcaccatt gctggaggaa gcttcatcaa agaagtaacc ttcgctatta aaaccagtca
    28201 gttgctctgg tttggtcagc cgattttcaa taatgaaacg actcatcaga ccgcgtgctt
    28261 tcttagcgta gaagctgatg atcttaaatt tgccgttctt ctcatcgagg aacaccggct
    28321 tgataatctc ggcattcaat ttcttcggct tcaccgattt aaaatactca tctgacgcca
    28381 gattaatcac cacattatcg ccttgtgctg cgagcgcctc gttcagcttg ttggtgatga
    28441 tatctcccca gaattgatac agatctttcc ctcgggcatt ctcaagacgg atccccattt
    28501 ccagacgata aggctgcatt aaatcgagcg ggcgcagtac gccatacaag ccggaaagca
    28561 ttcgcaaatg ctgttgggca aaatcgaaat cgtcttcgct gaaggtttcg gcctgcaagc
    28621 cggtgtagac atcaccttta aatgccagaa tcgcctggcg ggcattctcc ggcgtgaaat
    28681 ctggctgcca gtcatgaaag cgagcggcgt tgatacccgc cagtttgtcg ctgatgcgca
    28741 tcagcgtgct aatctgcgga ggcgtcagtt tccgcgcttc atggatcaac tgctgggaat
    28801 tgtctaacag ctccggcagc gtatagcgcg tggtggtcaa cgggctttgg taatcaagcg
    28861 ttttcgcagg tgaaataaga atcagcatat ccagtccttg caggaaattt atgccgactt
    28921 tagcaaaaaa agagaatgag ttgatcgata gttgtgatta ctcctgcgaa acatcatccc
    28981 acgcgtccgg agaaagctgg cgaccgatat ccggataacg caacggatca aacaccgggc
    29041 gcacgccgag tttacgctgg cgtagataat cactggcaat ggtatgaacc acaggcgaga
    29101 gcagtaatat ggcggtcaaa ttggtaatag ccatgcaggc cattatgata tctgccagtt
    29161 gccacatcag cggaagactt agcaaggtgc cgccgatgac cgttgcgaag gtgcagattc
    29221 gcagacacca gatcgcttta gggttgttca ggcgtaagaa gaagagattg ttttcggcat
    29281 aaatgtagtt ggcaacgatg gagctgaagg caaacagaat aaccacgagg gtaacaaact
    29341 ccgcacccca ggaacccatt aacacccgca tcgccttctg gataagctga ataccttcca
    29401 gtggcatgta ggttgtgccg ttacccgcca gtaatatcag catggcgctt gccgtacaga
    29461 tgaccagggt gtcgataaaa atgccaatca tctggacaat tccttgcgct gcgggatgcg
    29521 gaggccagga cgcagccgcc gctgccgcgt ttggcgtcga ccccattccc gcctcattgg
    29581 aaaacatact gcgctgaaaa ccgttagtaa tcgcctggct taaggtatat cccgccgcgc
    29641 cgcctgccgc ttcctgccag ccaaaagcac tctcaaaaat agaccaaatg acgtggggaa
    29701 gttgcccgat attcattacg caaattacca ggctggtcag tacccagatt aacgccatca
    29761 acgggacaaa cccctgcatg agccgggcga cgccatgaag accgcgagtg attgccagca
    29821 gagcaaagac agcaagaata atgcctgtca ccagcggagg aaaatcaaaa gaaaaactta
    29881 gggcgcgggc aacggcgttc gcctgaactc cgctgaaaat tatgccgtag gcgatgagca
    29941 aaaagacggc gaacagaacg cccatccaac gcatccccag cccgcgcgcc atataccatg
    30001 ccggtccgcc acgaaactgc ccattgacgt cacgttcttt ataaagttgt gcaagggaac
    30061 attcggcaaa cgaggtcgcc atgccgataa acgcggcaac ccacatccag aagacggctc
    30121 caggtccacc ggcggtaata gccagcgcaa cgccggccag gttgccgcta cccacgcgtg
    30181 ccgcaagact ggtacacaat gactgaaatg aggttaaacc gcctggctgt ggatgaatgc
    30241 tatttttaag acttttgcca aactggcgga tgtagcgaaa ctgcacaaat ccggtgcgaa
    30301 aagtgaacca acaacctgcg ccgaagagca ggtaaatcat taccgatccc caaaggacgc
    30361 tgttaataaa ggagaaaaaa tctggcatgc atatccctct tattgccggt cgcgatgact
    30421 ttcctgtgta aacgttacca attgtttaag aagtatatac gctacgaggt acttgataac
    30481 ttctgcgtag catacatgag gttttgtata aaaatggcgg gcgatatcaa cgcagtgtca
    30541 gaaatccgaa acagtctcgc ctggcgataa ccgtcttgtc ggcggttgcg ctgacgttgc
    30601 gtcgtgatat catcagggca gaccggttac atccccctaa caagctgttt aaagagaaat
    30661 actatcatga cggacaaatt gacctccctt cgtcagtaca ccaccgtagt ggccgacact
    30721 ggggacatcg cggcaatgaa gctgtatcaa ccgcaggatg ccacaaccaa cccttctctc
    30781 attcttaacg cagcgcagat tccggaatac cgtaagttga ttgatgatgc tgtcgcctgg
    30841 gcgaaacagc agagcaacga tcgcgcgcag cagatcgtgg acgcgaccga caaactggca
    30901 gtaaatattg gtctggaaat cctgaaactg gttccgggcc gtatctcaac tgaagttgat
    30961 gcgcgtcttt cctatgacac cgaagcgtca attgcgaaag caaaacgcct gatcaaactc
    31021 tacaacgatg cagggattag caacgatcgt attctgatca aactggcttc tacctggcag
    31081 ggtatccgtg ctgcagaaca gctggaaaaa gaaggtatta actgtaacct gacactgctg
    31141 ttctccttcg ctcaggctcg tgcttgtgcg gaagcgggcg tgttcctgat ctcgccgttt
    31201 gttggccgta ttcttgactg gtacaaagcg aataccgaca agaaagagta cgctccggca
    31261 gaagatccgg gcgtggtttc tgtatctgaa atctaccagt actacaaaga gcacggttat
    31321 gaaaccgtgg tgatgggcgc aagcttccgt aacatcggcg aaattctgga actggcaggc
    31381 tgcgaccgtc tgaccatcgc accggcactg ctgaaagagc tggctgagag cgaaggggct
    31441 atcgaacgta aactgtctta caccggcgaa gtgaaagcgc gtccggcgcg tatcactgag
    31501 tccgagttcc tgtggcagca taaccaggat ccaatggcag tagataaact ggcggaaggt
    31561 atccgtaagt ttgctgttga ccaggaaaaa ctggaaaaaa tgatcggcga tctgctgtaa
    31621 tcattcttag cgtgaccggg aagttggtca cgctacctct tctgaagcct gtctgtcact
    31681 cccttcgcag tgtatcattc tgtttaacga gactgtttaa acggaaaaat cttgatgaat
    31741 actttacgta ttggcttagt ttccatctct gatcgcgcat ccagcggcgt ttatcaggat
    31801 aaaggcatcc ctgcgttgga agaatggctg acatcggcgc taaccacgcc gtttgaactg
    31861 gaaacccgct taatccccga cgagcaggcg atcatcgagc aaacgttgtg tgagctggtg
    31921 gatgaaatga gttgccatct ggtgctcacc acgggcggaa ctggcccggc gcgtcgtgac
    31981 gtaacgcccg atgcgacgct ggcagtagcg gaccgcgaga tgccaggctt tggtgaacag
    32041 atgcgccaga tcagcctgca ttttgtacca actgcgatcc tttcgcgtca ggtgggtgtg
    32101 attcgcaaac aggcgctgat ccttaactta cccggtcagc cgaagtcgat taaagagacg
    32161 ctggaaggtg taaaggacgc tgagggtaac gttgtggtgc acggtatttt tgccagcgta
    32221 ccgtactgca ttcagttgct ggaagggcca tacgttgaaa cggcaccgga agtggttgct
    32281 gcattcagac cgaagagtgc aagacgcgaa gttagcgaat aaaaaaatcc ccccgagcgg
    32341 ggggatctca aaacaattag tgcgattcac caatcggcag aacggtgcga ccaaactgct
    32401 cgttcagcac ttcacccatc gccagataga ttgcgctggc accacagatc agaccaatcc
    32461 agccggcaaa gtggatgatc gcggcgttac cggcaatgtt accgatcgcc agcagggcaa
    32521 acagcacggt caggctaaag aaaacgaatt gcagaacgcg tgcgcctttc agcgtgccga
    32581 agaacataaa cagcgtaaat acgccccaca gacccaggta gacaccaagg aactgtgcat
    32641 ttggcgcatc ggtcagaccc agtttcggca tcagcagaat cgcaaccagc gtcagccaga
    32701 aagaaccgta agaggtgaat gcggttaaac cgaaagtgtt gccttttttg tactccagca
    32761 gaccagcaaa aatttgcgcg atgccgccgt agaaaatgcc catggcaaga ataataccgt
    32821 ccagagcgaa ataacccacg ttgtgcaggt taagcagaat ggtggtcatg ccgaagccca
    32881 tcaggcccag cggtgccgga ttagccaact tagtgttgcc cataattcct caaaaatcat
    32941 catcgaatga atggtgaaat aatttccctg aataactgta gtgttttcag ggcgcggcat
    33001 aataatcagc cagtggggca gtgtctacga tcttttgagg ggaaaatgaa aattttcccc
    33061 ggtttccggt atcagacctg agtggcacta accatccggc gcaggcaggc gatttgcagt
    33121 acggctggaa tcgtcacgcg ataggcgctg ccgctgaccg ctttaacccc atttagtgcc
    33181 gcgcctacag ggcctcccag ccccgcgccg cgcagcaaac catgcccaag tacgctcatt
    33241 gctgcgtggg tgcgtaaaat gcgggtcaat tggctggaaa gcaaatgcga cacgcctttt
    33301 gccaataatt tgtctttcat cagcagcggc agcagctctt ccagctcatt caccctggca
    33361 tcgactgcgt gcagaaactc ctgcttatgt tcctcgtcca ttttcttcca ggtattacgc
    33421 agaaattgtt ccagtaactg ttgctcaatt tcaaacgtag acatctcttt gtcggctttc
    33481 agcttcaatc gctttgaaac atcgagcaaa atggcccgat acaatttacc gtgtccacgc
    33541 agtttgttgg cgatactatc gccaccaaaa tgttgtaatt ctccggcaat cagctgccag
    33601 ttgcggcgat gttgctcggg atgtccttcc atcgatttaa acagttcgtt gcgcatcagt
    33661 acgctggaga ggcgagtttt gcctttttca ttatgggtga gcagccgggc gaaatttgcc
    33721 aattgttcct cactacaatg ctggagaaaa tccagatctg aatcattcag gtaattaaca
    33781 ttcatttttt gtggcttcta tattctggcg ttagtcgtcg ccgataattt tcagcgtggc
    33841 catatccgat gagttaaccg tatgaccgga aaaggtgatt tttgagacgc agcgtttatt
    33901 gtcgttatcg ctgttaatgt tgatccagtc agtggtttgc ccttctttta tttctgaagg
    33961 aatattcaga ctctgactgg cgctacgggc gactttgaaa taaaccgatg caccgcttaa
    34021 ctgcaaatcg ccatggtcgg ctgagagttg tatgcgttta acaattcggc aaacaggaag
    34081 tttcagcgcc agatcgttgg tttcgttacg tggcattgca atgacgccga ggattttatg
    34141 gtcgtttgcc tgcgccgtgc agcacagcat caggctaatc gccaggctgg cggaaatcgt
    34201 aaaaacggat ttcataagga ttctcttagt ggggagaggt aggggatgaa tacccactag
    34261 tttactgctg ataaagagaa gattcaggta cgtaatattt tctttttatt acaatttttc
    34321 tatgaatgtc ttggctgtca ttcattctct atatgaataa aattgctgtc agtttcgtgt
    34381 cttgttctgg catatcgcga aatttctgcg caaaagcaca aaaaattttt gcatctcccc
    34441 cttgatgacg tgggttacga ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa
    34501 aaatgaaatt gggcagttga aaccagacgt ttcgccccta ttacagactc acaaccacat
    34561 gatgaccgaa tatatagtgg agacgtttag atgggtaaaa taattggtat cgacctgggt
    34621 actaccaact cttgtgtagc gattatggat ggcaccactc ctcgtgtact ggagaacgcc
    34681 gaaggcgatc gcaccacgcc ttctatcatt gcctataccc aggatggtga aactctggtt
    34741 ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa
    34801 cgcctgattg gccgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg
    34861 ttcaaaatta ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg
    34921 gcaccgccgc agatttctgc tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac
    34981 ctgggtgaac cggtaactga agctgttatc accgtaccgg catactttaa cgatgctcag
    35041 cgtcaggcaa ccaaagacgc aggccgtatc gctggtctgg aagtaaaacg tatcatcaac
    35101 gaaccgaccg cagctgcgct ggcttacggt ctggacaaag gtactggcaa ccgtactatc
    35161 gcggtttatg acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt
    35221 gacggcgaaa aaaccttcga agttctggct accaacggtg atacccacct gggtggtgaa
    35281 gacttcgaca gccgtctgat caactacctg gttgaagaat tcaagaaaga tcagggcatt
    35341 gacctgcgca acgatccgct ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa
    35401 atcgaactgt cttccgctca gcagaccgac gttaacctgc cgtacatcac tgcagacgcg
    35461 accggtccga aacacatgaa catcaaagtg actcgtgcga aactggaaag cctggttgaa
    35521 gatctggtaa accgttccat tgagccgctg aaagttgcac tgcaggacgc tggcctgtcc
    35581 gtatctgata tcgacgacgt tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag
    35641 aagaaagttg ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct
    35701 gtagcaatcg gtgctgctgt tcagggtggt gttctgactg gtgacgtaaa agacgtactg
    35761 ctgctggacg ttaccccgct gtctctgggt atcgaaacca tgggcggtgt gatgacgacg
    35821 ctgatcgcga aaaacaccac tatcccgacc aagcacagcc aggtgttctc taccgctgaa
    35881 gacaaccagt ctgcggtaac catccatgtg ctgcagggtg aacgtaaacg tgcagctgat
    35941 aacaaatctc tgggtcagtt caacctggat ggtatcaacc cggcaccgcg cggcatgccg
    36001 cagatcgaag ttaccttcga tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat
    36061 aaaaacagcg gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat
    36121 gaaatccaga aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa
    36181 gagctggtac agactcgcaa ccagggcgac catctgctgc acagcacccg taagcaggtt
    36241 gaagaagcgg gcgacaaact gccggctgac gacaaaactg ctatcgagtc tgcactgact
    36301 gcactggaaa ctgctctgaa aggtgaagac aaagccgcta tcgaagcgaa aatgcaggaa
    36361 ctggcacagg tttcccagaa actgatggaa atcgcccagc agcaacatgc ccagcagcag
    36421 actgccggtg ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa
    36481 tttgaagaag tcaaagacaa aaaataatcg ccctataaac gggtaattat actgacacgg
    36541 gcgaagggga atttcctctc cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc
    36601 taagcaagat tattacgaga ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcaa
    36661 aaaggcctac aaacgcctgg ccatgaaata ccacccggac cgtaaccagg gtgacaaaga
    36721 ggccgaggcg aaatttaaag agatcaagga agcttatgaa gttctgaccg actcgcaaaa
    36781 acgtgcggca tacgatcagt atggtcatgc tgcgtttgag caaggtggca tgggcggcgg
    36841 cggtttcggc ggcggcgcag acttcagcga tatttttggt gacgttttcg gcgatatttt
    36901 tggcggcgga cgtggtcgtc aacgtgcggc gcgcggtgct gatttacgct ataacatgga
    36961 gctcaccctc gaagaagctg tacgtggcgt gaccaaagag atccgcattc cgactctgga
    37021 agagtgtgac gtttgccacg gtagcggtgc aaaaccaggt acacagccgc agacctgtcc
    37081 gacctgtcat ggttctggtc aggtgcagat gcgccagggt ttctttgccg tgcagcagac
    37141 ctgtccacac tgtcagggcc gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg
    37201 tcatggtcgt gttgagcgca gcaaaacgct gtccgttaaa atcccggcag gagtggacac
    37261 tggagaccgc atccgtcttg cgggtgaagg tgaagcgggc gagcatggcg caccggcagg
    37321 cgatctgtac gttcaggttc aggttaaaca gcacccgatt ttcgagcgtg aaggtaacaa
    37381 cctgtattgc gaagtcccga tcaacttcgc tatggcagcg ctgggtggcg aaatcgaagt
    37441 accgaccctt gatggtcgcg tcaaactgaa agtgcctggc gaaacccaga ccggtaagct
    37501 gttccgtatg cgcggtaaag gcgtcaagtc tgttcgcggc ggtgcgcaag gtgacttgct
    37561 gtgccgcgtt gtcgtcgaaa caccggtagg tctgaacgag aagcagaaac agctgctgca
    37621 agagctgcag gaaagcttcg gtggcccaac cggtgagcac aacagcccgc gttcaaagag
    37681 cttctttgat ggtgtgaaga agttttttga cgacctgact cgctaacctc cccaaaagcc
    37741 tgcctgtggg caggccaggg taaaaatagg gtgcgttgaa gatatgcgag cacctgtaaa
    37801 gtggcgggga tcactccccg ccgttgctct tactcggatt cgtaagccgt gaaaacagca
    37861 acctccgtct ggccagttcg gatgtgaacc tcacagaggt cttttctcgt taccagcgcc
    37921 gccactacgg cggtgataca gatgacgatc agggcgacaa tcatcgcctt atgctgcttc
    37981 attgctctct tctccttgac cttacggtca gtaagaggca ctctacatgt gttcagcata
    38041 tagggagcct cgggttgatg gtaaaatatc actcggggct tttctctatc tgccgttcag
    38101 ctaatgcctg agacagacag cctcaagcac ccgccgctat tatatcgctc tctttaacca
    38161 attctgtttt atcgattcta atcctgaaga cgcctcgcat tttt
//
LOCUS       DARNVM010000024        37416 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.024, whole genome shotgun sequence.
ACCESSION   DARNVM010000024 DARNVM010000000
VERSION     DARNVM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.024"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            3..365
                     /locus_tag="SI621_001185"
     CDS             3..365
                     /locus_tag="SI621_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063925.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKNMNKGSDE"
     gene            complement(550..1008)
                     /locus_tag="SI621_001186"
     CDS             complement(550..1008)
                     /locus_tag="SI621_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEI4063926.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1005..1922)
                     /locus_tag="SI621_001187"
     CDS             complement(1005..1922)
                     /locus_tag="SI621_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HEI4063927.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2068..2745
                     /gene="fetA"
                     /locus_tag="SI621_001188"
     CDS             2068..2745
                     /gene="fetA"
                     /locus_tag="SI621_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HEI4063928.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2732..3511
                     /gene="fetB"
                     /locus_tag="SI621_001189"
     CDS             2732..3511
                     /gene="fetB"
                     /locus_tag="SI621_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEI4063929.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3574..4428)
                     /gene="cnoX"
                     /locus_tag="SI621_001190"
     CDS             complement(3574..4428)
                     /gene="cnoX"
                     /locus_tag="SI621_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HEI4063930.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4489..5298)
                     /gene="ybbO"
                     /locus_tag="SI621_001191"
     CDS             complement(4489..5298)
                     /gene="ybbO"
                     /locus_tag="SI621_001191"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HEI4063931.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5288..5911)
                     /gene="tesA"
                     /locus_tag="SI621_001192"
     CDS             complement(5288..5911)
                     /gene="tesA"
                     /locus_tag="SI621_001192"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HEI4063932.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5882..6568
                     /locus_tag="SI621_001193"
     CDS             5882..6568
                     /locus_tag="SI621_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4063933.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6565..8979
                     /locus_tag="SI621_001194"
     CDS             6565..8979
                     /locus_tag="SI621_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4063934.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLSLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIIAGNW
                     PPKADEVSMEEGLAKRLNVGLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLHTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(9692..10786)
                     /gene="mnmH"
                     /locus_tag="SI621_001195"
     CDS             complement(9692..10786)
                     /gene="mnmH"
                     /locus_tag="SI621_001195"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEI4063935.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVDGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLHLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDVQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(10855..11781)
                     /gene="allS"
                     /locus_tag="SI621_001196"
     CDS             complement(10855..11781)
                     /gene="allS"
                     /locus_tag="SI621_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HEI4063936.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            12011..12493
                     /gene="allA"
                     /locus_tag="SI621_001197"
     CDS             12011..12493
                     /gene="allA"
                     /locus_tag="SI621_001197"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEI4063937.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            12571..13386
                     /gene="allR"
                     /locus_tag="SI621_001198"
     CDS             12571..13386
                     /gene="allR"
                     /locus_tag="SI621_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HEI4063938.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            13476..15257
                     /gene="gcl"
                     /locus_tag="SI621_001199"
     CDS             13476..15257
                     /gene="gcl"
                     /locus_tag="SI621_001199"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEI4063939.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            15270..16046
                     /gene="hyi"
                     /locus_tag="SI621_001200"
     CDS             15270..16046
                     /gene="hyi"
                     /locus_tag="SI621_001200"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEI4063940.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            16146..17024
                     /gene="glxR"
                     /locus_tag="SI621_001201"
     CDS             16146..17024
                     /gene="glxR"
                     /locus_tag="SI621_001201"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEI4063941.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            17194..18648
                     /locus_tag="SI621_001202"
     CDS             17194..18648
                     /locus_tag="SI621_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HEI4063942.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAENTGEQKVTG"
     gene            18709..20070
                     /gene="allB"
                     /locus_tag="SI621_001203"
     CDS             18709..20070
                     /gene="allB"
                     /locus_tag="SI621_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEI4063943.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            20127..21428
                     /locus_tag="SI621_001204"
     CDS             20127..21428
                     /locus_tag="SI621_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HEI4063944.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            21450..22595
                     /gene="glxK"
                     /locus_tag="SI621_001205"
     CDS             21450..22595
                     /gene="glxK"
                     /locus_tag="SI621_001205"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HEI4063945.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(22724..23509)
                     /gene="allE"
                     /locus_tag="SI621_001206"
     CDS             complement(22724..23509)
                     /gene="allE"
                     /locus_tag="SI621_001206"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEI4063946.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(23520..24755)
                     /gene="allC"
                     /locus_tag="SI621_001207"
     CDS             complement(23520..24755)
                     /gene="allC"
                     /locus_tag="SI621_001207"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HEI4063947.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(24777..25826)
                     /gene="allD"
                     /locus_tag="SI621_001208"
     CDS             complement(24777..25826)
                     /gene="allD"
                     /locus_tag="SI621_001208"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HEI4063948.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            26143..27810
                     /locus_tag="SI621_001209"
     CDS             26143..27810
                     /locus_tag="SI621_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEI4063949.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVSGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARPDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALALQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            27820..29079
                     /locus_tag="SI621_001210"
     CDS             27820..29079
                     /locus_tag="SI621_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEI4063950.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMDRGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQSIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQDLANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            29090..29905
                     /locus_tag="SI621_001211"
     CDS             29090..29905
                     /locus_tag="SI621_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEI4063951.1"
                     /translation="MTIIHPLLASRSAPNYCQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDTRSGRQFFACSDNLQLVTTAVSVNYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLMALGHTSGADTLLGFWLGQQLLQGKP"
     gene            29902..30795
                     /locus_tag="SI621_001212"
     CDS             29902..30795
                     /locus_tag="SI621_001212"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEI4063952.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(30932..31999)
                     /gene="purK"
                     /locus_tag="SI621_001213"
     CDS             complement(30932..31999)
                     /gene="purK"
                     /locus_tag="SI621_001213"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEI4063953.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFEMHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFN"
     gene            complement(31996..32505)
                     /gene="purE"
                     /locus_tag="SI621_001214"
     CDS             complement(31996..32505)
                     /gene="purE"
                     /locus_tag="SI621_001214"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEI4063954.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(32622..33344)
                     /gene="lpxH"
                     /locus_tag="SI621_001215"
     CDS             complement(32622..33344)
                     /gene="lpxH"
                     /locus_tag="SI621_001215"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEI4063955.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRQVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(33347..33841)
                     /gene="ppiB"
                     /locus_tag="SI621_001216"
     CDS             complement(33347..33841)
                     /gene="ppiB"
                     /locus_tag="SI621_001216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HEI4063956.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            34015..35400
                     /gene="cysS"
                     /locus_tag="SI621_001217"
     CDS             34015..35400
                     /gene="cysS"
                     /locus_tag="SI621_001217"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEI4063957.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(35436..35957)
                     /locus_tag="SI621_001218"
     CDS             complement(35436..35957)
                     /locus_tag="SI621_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEI4063958.1"
                     /translation="MPTVVTHAAVPLCIALGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWVFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(36065..36277)
                     /gene="ybcJ"
                     /locus_tag="SI621_001219"
     CDS             complement(36065..36277)
                     /gene="ybcJ"
                     /locus_tag="SI621_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HEI4063959.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(36279..37145)
                     /gene="folD"
                     /locus_tag="SI621_001220"
     CDS             complement(36279..37145)
                     /gene="folD"
                     /locus_tag="SI621_001220"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEI4063960.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         8649 a         9422 c        10191 g         9154 t
ORIGIN      
        1 aaatgaatga atttgagaag atttttaatg aaatgaatct ggaccgggca ttgctaccga
       61 tcctttttcg ttcgaaccgg tcaacagtgt ggaaatattt gagtggtgat agtacggctc
      121 ctgcctcagc tatgtcactg attatgctgt tgcagttaat ccagaaacga aaccccgatc
      181 tgttagccga atggttaacg ctttctgatt tcaccattcc gccagaagtt tacctggacc
      241 agcctgatta ctggaaaggg tgggtgtata cgcaacacaa ggtcaataaa aacgtcctgg
      301 aatatctaaa aaaacattat ccggatgaag accaaaaaaa catgaataaa ggtagtgacg
      361 agtaatttgc caatgccagt taatatagcg gaggcggcct acgattcggg cgcagacttc
      421 caggcatgat aagacgcgcc cgcgccgcat caggcatcag cgcataaccg ccggatgcgg
      481 cgtaaacgcc ttatccggcc tacaattcgg gcatattttg taggcatgaa aagacgcatc
      541 agcgtcgcat caggcgatga ccgcacggat aatcagcgtt atcccttcta tcgcaatcac
      601 ctcaacatgc gtacctgcac tgagatcctc gctggcgctg acaggccatg aactgtcgcc
      661 gacgcgcata tgacctcgcc cattgaccag cggtgattcc agcacaaaac gtcgaccaat
      721 cagctgctgc ccccgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg
      781 cgacaaccat ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattactcc
      841 ttgccactcc caacccagcg gcaccagcca gactaccagg ccggtaatca ccgcggccac
      901 gccgctccac aacaaataac catttccgcc cagcatctcg gctgccagca acaacccgcc
      961 gagactgagc cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt
     1021 ttgttggcgc tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg
     1081 gcctctaatg gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct
     1141 tcggtgtatt tctgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct
     1201 tcagacacca ttttggtggc gcgggcttct gcttcggcag aacgttcacg cgcttcagcc
     1261 tgtaaaaacg ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct
     1321 tcagctttga ggatttccgc ctgacggatc ccttccgctt cgaggatgta agcgcgtttg
     1381 gtacgttctg ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg
     1441 tcgcgaattt caatacgggt gactttaatc ccccacgggt tagtggcttc atcgacaata
     1501 cgcagcaggc gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa
     1561 cccagcacgg tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg
     1621 acttcatagg cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta
     1681 acgttggcgt tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc
     1741 atattgatct tgcgaccaat gcgatccata aacggcacca ccagacttag cccaggctgt
     1801 aacgttttgg tatagcgacc aaagcgttct actgtccact gatagccctg cggcacgatt
     1861 ttgacgcccg cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc
     1921 atcggaaaaa cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa
     1981 attatacctg attactgaaa gagagttccc ccttattcct gggaaggata aactgttttt
     2041 agtaaaaatc agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa
     2101 aacgtaggat atctggcggg tgatgcgaag attcttaata acatcaattt ttcgctgcgt
     2161 gctggcgaat ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa
     2221 atagttgctt cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc
     2281 agcacactaa agccagaaat ctaccgccag caagtctctt actgcgccca gacaccgacg
     2341 ctgtttggcg atacggtata cgataatctg atctttccct ggcagatccg taaccagcag
     2401 cctgacccgg ccatttttct cgattttctc gaacgcttcg ctttaccgga cagcattttg
     2461 acgaagaata tcgccgagct atctgggggt gaaaaacaac gcatctcatt aattcgtaac
     2521 ctgcaattta tgccgaaggt tttattgctg gatgaaatca ccagtgcgct ggatgaaagt
     2581 aataaacata acgttaatga gatgatccac cggtatgtgc gcgagcaaaa tattgccgtg
     2641 ctgtgggtga cacacgataa agacgaaatt aatcatgcgg ataaggtgat tacactgcaa
     2701 ccgcatgccg gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc
     2761 attggcactg gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact
     2821 ggcgctggag aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt
     2881 cggctatgtg ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt
     2941 gttatttatc tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa
     3001 agcttttatc tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct
     3061 gattctctct gggtcgattg aatttatccc gatgcaggtg atccctatcg ccgggatgat
     3121 agccggtaac gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtgat
     3181 tagcgaacag caacagattc aggagaaact gagtcttggc gcgacgccga aacaggcttc
     3241 agcgatattg attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa
     3301 aacggttggc ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga
     3361 tccggtgaag gcgattaaat atcagattat ggtgaccttt atgctgctct caactgccag
     3421 cctgtctacc attattgcct gctatttaac ctatcgtaaa ttttataatt cgcgccacca
     3481 gttggtggtg acgcaattga agaagaaatg atgcaatgcc ggatgcgcag catcgcatcc
     3541 ggcgttgtgg ttcatgtgcc gatcaacgga aactcaatac aacaacgcat acagctggcg
     3601 gcgatacttc gacgccagtg catcacctgt acccagcgca gcgaggatct cctggaacgt
     3661 tttacgcgtc tgaccgtctg cggcagtgag atctttacgc agatgcccaa acagtaactc
     3721 cagcgcctct tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc
     3781 ggcatcttct ggattctctg ccacctgctg ttgcagctgt tgaatttccg gcgtatcagc
     3841 cgcctgcttc agcagttcga tttgcgccac cagcccctga tagcgggtgt cctgatcctg
     3901 taacggaatg gttttcagca cggcttcagc atcttcagaa cggttcagcg caatcagcgt
     3961 ttctgccagc agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag
     4021 caatggcagg gcgtcggtgt aattgccttc ttgcatcagt tgcatcgcct gctgtgcttt
     4081 cagttcttct tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg
     4141 cggcccctgg aagccgtcta ccggttgccc gttctggaac agatacacgg tcggaatcgc
     4201 gcgcagacca aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat
     4261 aaattgcccg ttgtactgcg ccgcgaggct ttccagaatt ggggttaact gcaaacagtg
     4321 ctggctacgt tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac
     4381 ctgttgcagg ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc
     4441 tgttgtcgat attttctttg acatgggggc ttaagcgcgc gcttcaactt acccctgcaa
     4501 tattttgtcc atcacgcgcc ccggcagcag gcgcttaagc accattactg cccaggtcac
     4561 cagcgttacc ggatagcgca tcttcggttt ctcgctaata aaagcatggc gcactttgtc
     4621 gaccaccgct tccggcccca acgtaaagcg ggcggcgatg ccgggatttt cgacaggttt
     4681 atcactttgc gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat
     4741 caggctaact ttaattccgc tgtggcgtag ctccatgcgc agcgcatccg accacgcctc
     4801 cagcgcatat ttactggctg cgtaagcgcc gcgacccggc gtggagatta atcccatcac
     4861 cgatgaggtc atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat
     4921 ggtgagctgg tgtgcgccga aaaagttggc agaaaactgc tgttccatct gcgcacggct
     4981 gatggtggaa agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca
     5041 attatcggtc agggcgatca cttcgtcggc tgcgcgatca acactttctg gtgaatccag
     5101 atcgatcaac acgccggtaa atcccatgct gttcatgcgc tcaacatcat ccggtttccg
     5161 gcaacctgcc agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat
     5221 tccactggaa catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc
     5281 cgttgcttta tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa
     5341 taaacggctg ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga
     5401 ggtagacctc ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg
     5461 ggtaaatggc gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt
     5521 gcattaacaa tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt
     5581 gctcggtttg ttgtggctga aaaccacgca aaccgtcatt gccgcccagt tcaaccagca
     5641 cccagcgcgg ctgatgctgt ttcagcagag ccggaaggcg cgccagccct tgttgcgagg
     5701 tgtcgccgct gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca
     5761 aggcaggcca ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa
     5821 tcaataacgt gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca
     5881 aatgccagcg gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga
     5941 gcatgaactc tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc
     6001 actggtgggt gagtcgggat cgggtaagtc aaccttgctg gcaatcctcg ccgggctgga
     6061 tgacggcagc agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga
     6121 agcgcgggca aagttgcgcg cgaagcacgt aggttttgtt tttcagtcat ttatgttaat
     6181 tcctaccctt aacgcgctgg aaaacgtcga gcttccggcg ctgctgcgcg gtgagagtag
     6241 cgcggaaagt cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct
     6301 tgatcatctt ccggcacagc tttccggcgg cgaacagcaa cgagtggcgc tggcgcgagc
     6361 ctttaacggt cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca
     6421 gacgggcgat aaaattgccg acctgctgtt ttccctcaac cgtgagcatg gcaccacgtt
     6481 gattatggtg acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt
     6541 gaacgggcag ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgctcg
     6601 ccgtcgctat taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg
     6661 ggcaatatca gcgatcgcat ggagaagggc ttaagccagc aaagccggga gtttatggcg
     6721 ggcgatcggg cgttgcgcag ttcgcgcgaa gtgccgcaag cgtggctgga ggaagcgcaa
     6781 aagcgcggcc tgaaagtcgg caagcagctg actttcgcca ccatgacatt tgcaggcgac
     6841 acaccgcagc tggcgaacgt caaagcggtg gatgatatct atccgatgta tggcgatctg
     6901 caaaccaatc cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg
     6961 atggcactgc ttaacctgaa aacgggcgac accatcgacg tgggtgatgc caccttgcgg
     7021 attgccggag aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg
     7081 cgtctgatga tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc gggaagtcgg
     7141 gtcacctggc gctataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg
     7201 ttgttacctc agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg
     7261 ctggggcgct cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg
     7321 ctgctggcag tggcagcggt ggcggtggcg atgaatcatt actgtcgcag tcgctacgat
     7381 cttgtggcga tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc
     7441 ggtcagtggt tgatggtgct ggcgctttca gccgttaccg gcggggcgat aggcctgttg
     7501 ttcgaaaacg tgttgatggt gctgctcaag cccgttctgc ctgccgcact accgccagcc
     7561 agcctctggc cgtggctatg ggcgctgggc accatgacgg ttatctcgct gctggtgggg
     7621 ctacgaccgt accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg
     7681 gtagcgaacg tctggccgct caagttttat ctgccgattg tcagtgtggt ggttgtgctg
     7741 ctgctcgccg gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta
     7801 gtactggctt tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg
     7861 acgctgaaat cgctatctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca
     7921 acgttaagcc agctttcggc attttcgctc tcctttatgc tgttggcact gctgctggtg
     7981 ctgcgtggcg atctgctcga ccgctggcag cagcagctac ctccagaaag cccgaactac
     8041 tttttaatta acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat
     8101 cagatagtcc cggaatcgtt ttatccggtg gtgcgggcgc ggctgacggc gattaacgat
     8161 aagccgacag aaggtaatga agatgaggcg cttaaccgcg aactcaatct tacctggcaa
     8221 aatacgcggc ccgatcataa tccgattatc gccggtaact ggccgccaaa agccgatgaa
     8281 gtgtcgatgg aagaggggct ggcgaaacgc ttaaacgttg gcctcggcga tacagtcacc
     8341 tttatgggcg atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg
     8401 gaaagtctgc ggcctaattt ctattttatt ttcccggaag gggcattaga cgggcaaccg
     8461 cagagctggc ttaccagttt ccgctgggag aatggcaatg gcatgttgac gcaactcaac
     8521 cgccagttcc cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag
     8581 gtgctggagc aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac tgcctgcggt
     8641 atgttgctgt tgctggcaca ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg
     8701 gtgtggcgca cactcggtgc ggggaaaaaa ctgctgcaca ccacgttgtg gtgtgaattc
     8761 gccatgctcg ggtttgtttc tggcctggtg gccgcaattg gtgcggaaac ggcgctggca
     8821 gtgttgcagg cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt
     8881 ctgccgtgca gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg
     8941 gttaagggta aggcgctgtt caggcagttt gcgggatgat gacaggtgtt agattgcctg
     9001 atgcgctacg cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg
     9061 taggccggat aaggcgttta cgccgcatct ggcatgaaca gtatgcattt tggcaataaa
     9121 tatgaaacca actgtcatgt cggttttttt aaaggccgaa ggtggcaaac cttcggcctt
     9181 agctacgttt cctcagttat tcaataaact gacgaatcag ttccagcagc gcgattagcg
     9241 ccttaagaac gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag
     9301 aaagacaatc acagcttacc cttacgttgc ctgtaactat gattttcttc atgtgctaga
     9361 tgaggactgc ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag
     9421 gacttaaaag tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc
     9481 gggattgtat cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt
     9541 gctagatgag gactgtttca tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa
     9601 aaaaaccgac tgtcatgtcg gtttttttat atctgtattc tggtgcaacc attctgttct
     9661 cacgacaatc gccgtttcag aataccgccc atcactgcgc cttaacccat tccgccactt
     9721 ccgcccactc accgcgaaag acaacttttt cagctttttt ctcaagctga tagcgataca
     9781 tcgggtcgta atattcttca agtaacggca ccagccaggc cagatgaccg tcggtgctgc
     9841 cggtggtgag ttgcgttgtc agcgcagcat ccagccttgc agccagttcg ttatatcgct
     9901 gtaaccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg caatactcct
     9961 gccagccctg tacgtcgccg tacgcgtggg taaaatcatg atgcatacgc aagaaatact
    10021 cttcgttcag gcgttcaaga cggatctcaa acggatcttc taccaccgca atcgccgcct
    10081 gagtcattcg ctcgcgcagg cattccggca ggtgattcga accgatcatc cggctttcat
    10141 cttccagcac ccacaggtgc aaatcctgac gggcgtcggt ttttagcatt tcggcagcca
    10201 gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa cgtgcggcca aacgccgaac
    10261 cgcgatgacg cgccagccct tccagatcaa caccgttcgg ctgttgctgc actaacagcg
    10321 ttttaccgct gccggtacaa ccgccaatca gcactatcgg tttttgtgcc agttcaatgg
    10381 tcgcctgaat cgcggtctgg cgcagtgcct tataaccgcc gtctaccagc ggataatcaa
    10441 tccccgcttc atgcaaccag cgttgcacaa tatgtgaacg ttgaccgccg cgggcgcagc
    10501 agagaatacc ttgcggattt tgcaggcacg ctgcccgcca ggcgtccata cgctgctgac
    10561 gaatttcacc cgccaccagt ttatgaccca gcgccagcgc cgcgtctgac ccttgctgtt
    10621 tataacaggt gccaacggcg gcgcgttcat cgttattcat taacggcagg ttgatagcgg
    10681 caggcattgc gccttgctca aactcgatgg gggcgcgaac atcaataatg ggcgtatcag
    10741 caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac
    10801 ctcaaatcag cttgcacgct attttacgcg ccaacgcgca aggaaacttg atttttaact
    10861 gcgtgggttg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa
    10921 ggtcacaata tcttctaccg ctttgccgct gccaaatttg cgccatgcca gactcaatgg
    10981 cgaaggcggg cgcatcgttg ggattacccg gctgaccagt tgttgattat cgatcattga
    11041 ctggcaaagt gattttggca aaaaaccaat gccaacgccc gccagatggg cggcgatttt
    11101 cgtttccata tcaggaacaa taatctcttt ttgccctggc aatcgccagg cgacgcgttt
    11161 ggttaaggtg cgggcgctgt cttcaatatt gaccgccgga aagcgacgca actgagcttc
    11221 tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa agcgccattg
    11281 caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcggttc ccgtgacgcc
    11341 gatagccagt gaaaaacctt cgtacaatag cgagtcccag acgcccatat agatttgccg
    11401 ggagatgtgg aactgggtaa aggggtaacg ttcattcagc cacgccagca gctgggcgac
    11461 ggcctgggga ttgtagagca ggttgttgat gacaatattc acctggcgtt ccacgccatc
    11521 attcacctgt tgcagctcgc tgggcatact ttccagccag ctcagccagt ctctggcctg
    11581 agaaagtaga tgctcgccag ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag
    11641 cgctactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt
    11701 acataatcgt tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg
    11761 caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag ttgcgtgatt
    11821 ttcttatcct ctgatttatc agtattttta catgataacc ctgttcaatt tgtggactaa
    11881 atctagtttt ggaaaaatat tccaactttt gtgttgatgt tgttctctta aggttttaga
    11941 ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaatggaat tatgttacaa
    12001 ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta gtgcttatgg
    12061 cgacgtaatc gaaacgcaga aacgggattt tttccatatt aacaatggac tggtggagcg
    12121 ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta tcagcattaa
    12181 ccgcgcgcaa ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc atccgctggg
    12241 tactcaggcc tttatcccga tgaaaggtga agtgtttgtg gtggtcgtgg cgttaggtga
    12301 cgacaaacca gacctgtcaa cgctgcgggc gtttattacc aacggcgaac agggggtgaa
    12361 ttaccatcgt aacgtctggc atcatccact tttcgcctgg cagcgcgtca ccgattttct
    12421 gaccatcgat cgcggcggca gtgacaattg tgatgttgaa agtattcctg aacaggaact
    12481 ctgttttgcg tgacgcctgc aaccgacttg cataagataa actaattgtt cattgtttat
    12541 gctcacttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg cggcaggcca
    12601 ggacaggcgg agcctgtggc acagaagggc gcacaggcgt tagagcgggg aattgcgatt
    12661 ctgcaatatc tggaaaaaag tgggggaagt tcatcggtta gcgatatttc tctcaatctg
    12721 gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaggcagc ggattttgtc
    12781 taccaggaca gtcagttagg ctggtggcat ataggattag gtgtttttaa cgtcggtgcg
    12841 gcgtacatcc ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg ccgcctgatg
    12901 ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc agtattaatt
    12961 ggtcagttag agtgtaaatc gatggtcagg atgtgtgcgc cgctgggcag tcgtctgccg
    13021 ctgcatgctt ccggtgcggg caaggcgctg ctttatccgc tggcggaaga ggagttgatg
    13081 agcatcattc tgcaaaccgg tttgcagcag tttacgccaa cgacgcttgt ggatatgccc
    13141 accttgctga aggacctgga acaagcgcgt gaactgggct ataccgtaga taaagaagag
    13201 catgttgtag gcctgaattg catagcttca gcaatttacg atgatgtagg tagtgttgtt
    13261 gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt tgtcagtcag
    13321 ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact gaaagcacat
    13381 ccatagtgtc tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa tttgaaagtt
    13441 ggaaaatttt tccaattaat agaggtagga ataaaatggc aaaaatgaga gccgttgacg
    13501 cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt ccgggagctg
    13561 caatcaatcc gttctattca gcgatgcgta agcacggcgg tattcgtcac attctggcgc
    13621 gtcatgtgga aggtgcttcg cacatggcgg aaggttatac ccgcgcaacg gcagggaata
    13681 tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc gcgctctatt
    13741 ccgcttctgc tgattccatt cctattctgt gcattaccgg ccaggcaccg cgcgcccgtc
    13801 tgcataaaga agattttcag gccgtagata ttgaagcaat tgctaaaccg gtcagcaaaa
    13861 tggcggttac agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag gcgtttcacc
    13921 tgatgcgttc tggtcgcccg ggtccggtgc tggtggattt accgttcgac gttcaggttg
    13981 cggaaatcga gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa cctgctgcca
    14041 gccgtatgca gatcgaaaaa gctgtagaaa tgttaattca ggccgaacgt ccggtgattg
    14101 ttgccggggg cggggtaatc aatgctgacg ctgctgcact gttacaacag tttgctgaac
    14161 tgaccagcgt tccggtgatc ccaacgctga tgggctgggg ctgtatcccg gatgaccacg
    14221 aactgatggc cgggatggtg ggtctgcaaa ccgcgcatcg ttacggtaac gcaacgctac
    14281 tggcgtccga catggtgttt ggtatcggta accgttttgc gaaccgtcat accggttcgg
    14341 tagagaaata caccgaaggg cgcaaaatcg ttcatatcga tattgagccg acgcaaattg
    14401 gccgcgtgct gtgtccggat ctcggcattg tctctgatgc taaagcggcg ctgacactgc
    14461 tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt aaagaatggg
    14521 tcgccgactg ccagcagcgc aaacgcactt tgctgcgcaa aacccacttc gacaacgtgc
    14581 cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc gatgtttgtt
    14641 atgtcaccac cattggtctg tcacaaatcg ctgcggcaca aatgctgcat gtctttaaag
    14701 accgccactg gatcaactgt gggcaggcag gcccgttggg ctggaccatt ccggcggcgc
    14761 tgggcgtgtg tgccgctgat ccagagcgca aagtggtggc gatttccggt gacttcgact
    14821 tccagttcct gattgaagaa ttagccgttg gcgcacagtt caacattccg tacatccatg
    14881 tactggtcaa taacgcctat ctgggtctga ttcgccagtc gcagcgcgcg tttgatatgg
    14941 attactgcgt acagctcgcg ttcgaaaata ttaactccag cgaagtgaac ggttacggcg
    15001 tcgaccacgt aaaagtagcg gaaggtttgg gttgtaaagc gattcgcgtt ttcaaaccgg
    15061 aagatatagc accagccttt gaacaggcga aagccttaat ggcgcaatat cgggtaccgg
    15121 tagtcgtgga agttattctc gagcgtgtga ccaatatttc gatgggcagc gaactggata
    15181 atgtcatgga atttgaagat atcgccgata acgcagtgga tgcaccaact gaaacctgct
    15241 tcatgcacta tgaataaggg agataaataa tgttacgttt ctctgctaat ttatcgatgt
    15301 tatttggtga atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt ggttttcgcg
    15361 gcgttgaatt tatgttccct tatgactacg acattgaaga attaaaacag gtgctggcga
    15421 gtaataaact cgaacatacg ctgcacaatt taccggcagg tgactgggcg gcgggcgagc
    15481 ggggtattgc ctgtattcct ggccgtgaag aagagtttcg ggatggcgta gcagcagcga
    15541 ttcgttatgc ccgtgcgctg ggtaataaaa aaattaactg tctggtcggt aaaacgccgg
    15601 ctggtttcag cagtgaacag attcacgcaa cgcttgtaga aaacctgcgt tatgccgcga
    15661 atatgctgat gaaagaagat attttattac tgattgaacc tattaaccat tttgatattc
    15721 ctggtttcca tctcaccgga actcggcagg cgctgaaatt gattgatgat gttggttgct
    15781 gcaatttgaa aattcagtat gacatttatc acatgcagcg gatggaaggt gaattaacca
    15841 acactatgac gcaatgggcc gataaaattg gtcacctgca aattgccgat aatccgcatc
    15901 gcggcgaacc ggggaccgga gaaattaatt atgattatct ctttaaggta atcgaaaatt
    15961 ctgactacaa cggttgggtt gggtgtgaat ataaacccca aaccaccacg gaagccggtt
    16021 tacgctggat ggatccgtac cgttaaaacg taacgctatt cagacaatgc ttttttaggc
    16081 cgctaagttg gcaggggatc gtgttgtctg aattcaggaa aagcgaaatt taaaagaggt
    16141 taattatgaa actgggattt attggcttag gcattatggg tacaccgatg gccattaatc
    16201 tggcgcgtgc cggtcatcaa ctgcatgtca caaccattgg tccggttgct gatgaattac
    16261 tgtcactggg tgccgtcagt gttgaaactg cgcgccaggt aacggaagca tcggacatca
    16321 tttttattat ggtgccggat acgccacagg ttgaagaagt tctgttcggt gaaaatggtt
    16381 gtaccaaagc ctcgctgaag ggcaaaacca ttgttgatat gagctccatt tccccgattg
    16441 aaactaagcg tttcgctcgt caggtgaatg aactgggcgg cgattatctc gatgcgccag
    16501 tctccggcgg tgaaatcggc gcgcgtgaag ggacgttgtc gattatggtt ggcggtgacg
    16561 aagcggtatt tgaacgtgtt aaaccgctgt ttgaactgct cggtaaaaat atcaccctcg
    16621 tgggcggtaa cggcgacggt caaacctgca aagtggcaaa tcagattatc gtggcgctca
    16681 atattgaagc ggtttctgaa gccctgctgt ttgcttcaaa agccggtgca gatccggtac
    16741 gtgtgcgcca ggcgctgatg ggcggctttg cttcctcacg tattctggaa gttcacggtg
    16801 agcgcatgat taaacgcacc tttaatccag gttttaaaat tgcgctgcac cagaaagatc
    16861 tcaacctggc actgcaaagt gcgaaagcac tggcgctgaa cctgccaaac accgcgacct
    16921 gccaggaatt gtttaatacc tgtgcggcaa acggtggcag ccagttggat cactctgcgt
    16981 tagtgcaggc gctggaatta atggctaacc ataaactggc ctgatacccg caataaaaat
    17041 ggccgatatc agaaaatgaa tcggccagca atattaaaaa aagaaagcag ccaaagatgt
    17101 tgcttcagta ttaaaaataa tatttttatt ttatttgttc ctcatagcta gattaaaaca
    17161 gcgttattcg atacgtgaaa ttaagaggga tttatggaac atcagagaaa actattccag
    17221 caacgcggct atagcgaaga tctattgcca aaaacgcaaa gccagcggac ctggaaaaca
    17281 tttaactatt ttaccttatg gatgggttcg gttcataacg ttcccaatta tgtgatggtc
    17341 ggcggctttt ttattctcgg cttgtctacc tttagtatta tgctggcaat tatcctcagc
    17401 gcctttttca ttgccgcggt aatggtatta aacggtgctg cgggcagtaa atacggtgtg
    17461 ccttttgcca tgatcctgcg tgcttcttac ggcgtacgtg gtgcactgtt tcccggatta
    17521 ttaagaggcg ggattgctgc cattatgtgg tttggcctgc aatgttacgc tggatcgctg
    17581 gcctgcttga tcctgattgg caaaatctgg ccgggatttt taactctcgg tggtgatttt
    17641 accctgttag ggctttctct accgggctta attactttct tactcttctg gctggtcaac
    17701 gtcggaatcg gtttcggtgg tggcaaagtt ttaaataaat tcactgccat tcttaacccg
    17761 tgcatctata tcgttttcgg cggtatggcg atttgggcga tttcgctggt cgggctcggt
    17821 ccaatctttg actacattcc gagcggtatt cagaaagcag aaaacagtgg cttcctgttc
    17881 ctggtggtga ttaacgcggt agttgcggtc tgggcggcac cggcagtgag cgcatccgac
    17941 tttacgcaaa acgcccactc gtttcgtgag caggcgctgg ggcaaacgct cggtttagtt
    18001 gtggcctata ttctgtttgc ggtcgccggg gtatgtatta ttgccggagc cagtattcac
    18061 tacggcgctg acacctggaa cgtgctggat attgttcagc gttgggatag cctgtttgcc
    18121 tcgttctttg cggtactggt tattctgatg acgaccattt ccactaacgc caccggtaat
    18181 attattccgg ctggttatca gattgccgct attgcaccga cgaaactgac ctataaaaac
    18241 ggcgtactga ttgccagtat tatcagcctg ctgatctgcc cgtggaaatt aatggaaaat
    18301 caggacagta tttatctgtt cctcgatatt atcggcggaa tgctgggtcc ggtaattggt
    18361 gtcatgatgg cgcattattt tgtggtgatg cgtgggcaaa ttaatcttga tgaactgtac
    18421 accgcacctg gcgattataa atattacgat aacggtttta acctcactgc gttttcagta
    18481 actctggtgg ccgttatttt atctcttggc ggtaagttta ttccctttat ggaaccgtta
    18541 tctcgtgttt catggtttgt cggcgtcatc gtcgcctttg cggcctacgc cttattaaag
    18601 aaacgtacta cagcagaaaa cacaggagaa caaaaagtca caggttaatt aatcccgata
    18661 ttgaacattg agttaaaaaa ccaatctgta ttttacaagg agtttgttat gtcttttgat
    18721 ttaatcatta aaaacggcac tgttatttta gaaaacgaag ctcgcgttgt agatatcgcc
    18781 gttaaaggcg gaaaaattgc tgctatcggt caggatctgg gcgatgcaaa agaagtgatg
    18841 gatgcgtctg gtctggtggt ttcgccgggc atggttgatg cgcacaccca tatttctgaa
    18901 ccgggtcgta gccactggga aggttatgaa accggtactc gcgcagcggc aaaaggtggt
    18961 atcaccacca tgatcgaaat gccgctcaac cagctgcctg caacggttga ccgcgcttca
    19021 attgaactga agttcgatgc tgctaaaggc aagctgacta tcgatgcggc gcaactcggt
    19081 ggcctggtgt cttacaacat cgaccgtctg catgagctgg atgaagtggg cgttgtcggc
    19141 ttcaaatgct tcgttgcgac ctgtggcgat cgcggtatcg acaacgactt ccgtgacgtc
    19201 aatgactggc agttcttcaa aggtgcgcag aagctgggcg aactggggca gccggtgctg
    19261 gtgcactgcg aaaacgcact gatctgtgat gcactgggcg aagaagcgaa aagtgaaggt
    19321 cgcgtaactg cccatgacta tgtggcttcg cgtccggtat ttaccgaagt ggaagcgatt
    19381 cgccgcgtac tgtacctggc gaaagttgcc ggttgccgtc tgcacatttg ccatatcagc
    19441 agcccggaag gtgttgaaga agtgactcgt gcacgtcagg aaggtcagga tgttacctgt
    19501 gaatcctgcc cgcattactt tgtactggat accgatcagt tcgaagaaat cggtactctg
    19561 gcgaagtgtt caccgccgat ccgcgatctg gaaaaccaga aaggcatgtg ggaaaaacta
    19621 tttaacggtg aaatcgactg cctggtttct gaccactcac catgcccgcc ggaaatgaaa
    19681 gccggcaaca tcatggaagc atggggcggt attgccggtc tgcaaaactg tatggacgtg
    19741 atgttcgatg aagcggtaca gaaacgcgga atgtctctgc caatgttcgg caaattaatg
    19801 gcgactaacg cagcagatat tttcggtctg cagcaaaaag gccgtatcgc cccaggaaaa
    19861 gatgccgact tcgtcttcat tcagcctaat agcagctatg ttcttaccaa tgacgatctg
    19921 gaatatcgcc acaaagtcag cccgtatgtt ggccgtacca ttggcgcgcg tatcacgaaa
    19981 accatcttac gtggtgatgt gatttacgac attgaacagg gcttccctgt tgcgccgaaa
    20041 ggtcaattta tccttaaaca tcagcagtaa tctggcccct gcaatgcccg tccttgcggc
    20101 gggcattctc cggttaaggt gtgtttatgt tcaattttgc agtcagccgc gaaagcctgt
    20161 tatcaggatt tcagtggttt ttctttattt tttgcaacac tgttgtggtt cctcctacgc
    20221 tactttctgc ttttcagttg ccgcaaagca gcctgcttac gctcacgcaa tatgcttttc
    20281 ttgctaccgc actggcctgc ttcgctcagg cgttctgcgg tcatcgtcgc gctattatgg
    20341 aagggccggg tggcctgtgg tggggaacca tccttacgat cacccttggc gaagcatcgc
    20401 gcgggacacc gatcaacgat atcgctacca gcctggcggt gggaattgcg ctctctggcg
    20461 tgctgacgat gttgattggt tttagcggat taggtcatcg tctggcgcgg ttatttacgc
    20521 cgtcggtgat ggtcttgttt atgttgatgc tgggcgcgca gctgaccact atctttttca
    20581 aaggtatgct cgggctgccg tttggcatag ccgacccgaa ttttaaaatt cagttaccgc
    20641 cgttcgcgct ttcggtcgcg gtgatgtgcc tggtactggc gatgattatc tttctgccgc
    20701 aacgttttgc ccgttatggc ctgctggtcg gtacgataac cggctggttg ttgtggtact
    20761 tttgctttcc ttcttcgcat tcgctctccg gtgagttgca ctggcagtgg ttcccgctgg
    20821 gcagtggagg tgctttgtcg ccggggatta ttctgacggc ggtgattaca ggtctggtaa
    20881 atatcagcaa tacctacggt gcgattcggg gcacggatgt tttttatccg cagcagggcg
    20941 cggggaatac gcgttatcgt cgtagctttg tggcgaccgg atttatgacg ctgataaccg
    21001 taccgctggc ggtaattcca ttttcaccgt ttgtttcatc cattggttta ttaacccaga
    21061 ctggcgatta cacgcggcgt tcgtttattt atggcagcgt tatttgcctg ctggtggcgc
    21121 tggttccggc actcacacga ctgttttgca gtatcccttt acccgtgagt agcgcggtca
    21181 tgctggtttc ttatctgcct ttactctttt ccgcgctggt gtttagccag caaataacct
    21241 ttaccgctcg caatatttat cgactcgcat tgccgttatt tgtcggcata tttttaatgg
    21301 cattaccgcc tgtgtatctg caagaccttc cattaacgct tcgtcctctg ctcagtaacg
    21361 gcttactggt cgggatttta ctggctgttc ttatggataa tcttattccg tgggaacgca
    21421 tcgaataatt tgttgaaaaa ggattgataa tgaagattgt cattgcgcca gactcgttta
    21481 aagagagctt aagtgcagaa aaatgttgtc aggcaattaa agccgggttt tcgaccctct
    21541 ttcccgatgc gaactatatc tgtttgccga tagcggatgg cggcgaaggg acggtggatg
    21601 cgatggtcgc cgcgacgggc ggcaacatcg tgacgcttga agtctgcggg ccgatgggcg
    21661 aaaaagtgaa tgctttttat ggccttaccg gcgacgggaa aacagcggtg attgagatgg
    21721 cggctgcaag tggtctgatg ctggtcgcgc ctgaaaagcg taatccgttg ctggcctcca
    21781 gttttggtac gggggagtta attcgtcatg cgctggataa cggcattcgc catattattc
    21841 tcggcatagg cggcagtgcg acggtcgacg gcggtatggg catggcgcag gcgcttggtg
    21901 tgcgttttct cgatgccgac ggtcaggtgc tggcagctaa cggcggcaat ttagcgcgcg
    21961 tggcaagcat tgagatggat gaatgcgatc cgcgtctggc gaactgccat attgaagtcg
    22021 catgtgatgt tgataatccg ctggtagggg cacgcggcgc ggcggcggtg ttcggcccgc
    22081 aaaaaggggc aacgccggaa atggtcgaag agctggagca ggggctgcaa aattacgccc
    22141 gtgttttaca acagcaaact gaaatcaacg tctgtcagat ggcgggcggc ggcgcggcgg
    22201 gcggtatggg tattgcggcg gcggtattcc tcaatgcgga tattaaaccg ggtattgaga
    22261 ttgtactgcg ggcggtcaac ctggagcagg cggtgcaggg tgcagcgctg gtgatcaccg
    22321 gggaagggcg catcgattcg caaacggcag gtggtaaagc gccgctgggc gtggcgtcgg
    22381 tggcgaagca gtttaatgtc ccggtgattg ggattgctgg cgtattgggt gatggcgtgg
    22441 aagtggtgca ccagtacggc attgacgcgg tgttcagcat tttgccacgt ctggcacctt
    22501 tagccgaagt gctcgccagc ggtgaaacca atctcttcaa cagcgcacga aatattgcct
    22561 gcgccattaa aataggtcag ggaattaaaa actgactctt gccattagag cggatgcatt
    22621 ttatcccgca tccgttttta cctgaattta ccgcaaaaaa agccacggta taaaccgtgg
    22681 caaaatccaa catagctaaa aataatcagg cgagtggtat gacttaaatt tctacgtcgc
    22741 ggttgcaatc ttttgagtaa atataactaa acgcttcacc acgccctacg ccataaccgg
    22801 cctgtaagga ataagcgccc ataaagatgt aatcgccttt tttcaccggg atccagttat
    22861 tgtcgaggtt ataaaccccc tgaccggaaa gaatataggc accgtgttcc tggacgtgag
    22921 tttcgatata accgtggctg gcacctggcg caaaagagag gatatgcatg ttcatatcaa
    22981 aacctaactc tttgggcaga aaatccagca gaataacatc gtccatgcct tcataatgaa
    23041 tgcgttcgag ttcgctggca ttgccagaaa ccagccacgg cgcatgacct tctaccggaa
    23101 catagcgacg cttatataaa aagatttggc tgtcttcggc ctgggcgtta acaaacgtca
    23161 ttaaggagcc tggtgggcaa taaagatagc cgccttcgct taaggcaaat gttttacctt
    23221 cggctttggc agtgatattt ccagagatca catacaggaa cgtttcaatg ccttcgcccc
    23281 cgaagccctg ttggttgcca ccgttttgat gcagtgtgac cagataatca acaaaagagg
    23341 cacccagctt tggcgtggag aggattgtcg cgtcacaatt ttcaaagccc ggaataatat
    23401 tttttaccag accgtctggt gttaacagtg cgaaattacc gtgtttaaca atcgcacggt
    23461 tagccagtaa atcatcgcgg taaccggtga cgttatttaa atatcccatt tatgactcct
    23521 tatttctgcc aggcaagttg ataaagcatg agtgccaacg ttttgacccc ttcggcaagg
    23581 tcggtaatat tggtgcgttc cgccgggtta tggctgatcc cgttgatgct ggggataaaa
    23641 atcatgcagg ttggtacgcg aggcgcgaaa atttgcgcgt cgtgcccggc accactgtgc
    23701 atcacccggt aattcagttt ttctctttca cacaattctg tcagggtggc gaccagttcc
    23761 ttgttcatcg gcacgggttc ttcgtccatc cataaatcga tatcaatacc aatgtccatt
    23821 tcatcgcaaa tcgcccgcat gtcgttttct aactgttggg tgaaatcgcg cagcacggca
    23881 gcatcggtat gacgacaatc aatggtgaac gtggttttac ccggcaccac attcaccgta
    23941 ttcgggcgcg gctctacttt gccaaaggtc agaaccagcg gatcgcccat ttttttcgct
    24001 ttttcgacag actgatggca aatgcgactg aaagcgtaaa ccgtatcacg acgataaccc
    24061 atcggcgtgg tgcctgcatg gtttgattcg ccgttcagcg tcaccgtata acgacgctgc
    24121 ccgacaattg cattcaccac gccaattgac tgcccattac tttccagcac acagccctgt
    24181 tcgatatgca gttcgacaaa ggctttaata tcctgacgcg gagttagtgg ggcgttcggc
    24241 agagtaaatc cacaagcctt catcgcatcg acaaaactat ttcctttggc atcacagata
    24301 ttccgcacgt cgtcaggatt cgccagccca aagatatttt tactgcccca gaagacatac
    24361 gggaagcggc tgccttcttc ttctgccatc gccaccactt cgaccgtacg taacggcgcg
    24421 ccgtattgcg ttttcaacca gtcaattgcc agccacgccg ccagcgcgcc gaattgcccg
    24481 tcgaggttac cgccgttaac cacggtatcg atatgcgaac cgctcagaac cacttcctgt
    24541 ggatattcgg tgccactcag gcgaccgtat aaatttccca cttcatcgaa acgtgtttcc
    24601 agcccgcttg ctgccattct ttttttaaat tgctgctggg tttccagcca ttccggcgaa
    24661 taaagtaaac gggtcatacc acccgttggg tcagcgccaa aagaggaaag ccagggcagc
    24721 gtttcttcta tggcttgacg gaaatgtgta atcataagaa agtcctgtct caataattat
    24781 tgcgcaaagg gatttttcgt ttcgtatgac gtgttataaa gtgcgtcgga aattaaatac
    24841 tggtaaatat catcaacaat ttcgatgcct tcgacggcgg ctttgcgttg tttaatatcc
    24901 tgatcctgtc cgggataata aacttggtta aaaccgggcg cgggggttat ggcatttaat
    24961 tcgcgcatgg tctggctaag atgttgacgg aatagttcgc tggaggagaa aaagttcggg
    25021 ttaataacga tatgtaattg ccccaaatta cgcccggcgt gtaaatcgtc atacatcgaa
    25081 ctaacctgtc gcccgaacgg taagccgagt aagacgcctg agaggacgtc aatcatcatc
    25141 atcaggccat accctttcgg cccggcggcg gggagcagag catgtaccgc gaacggatcg
    25201 gttgttggtg caccgttttt atcgaccgcc caggtatccg ggatagacat attacgcgag
    25261 cgggcgtcga gcacttttcc ccatgcctgt acggtagtcg ccatatcaaa ggtaaggatc
    25321 tcgtcgcctt ctcccggcgc ggcaaaggcc agtgggttag taccgtagta aatttccgcg
    25381 ccgccaaacg gcaccaccat tggatcggac tggcacatcg aaatgccaat taatccggcg
    25441 cgggctgcct gctgcacaaa ataagagatt gcgccgctgt gacccatccg gctgataccg
    25501 accaccgcaa cgccattttg ctgggcggtt ttgatggcat gttccatacc cattttcgcc
    25561 gcgacctgtc cggcggcatt gtcggcatgt aaaattgccg agcacggccc ggtttcctca
    25621 agacgaaact ccggttcgcg gttggtgccg ccttttgaaa tgcgttccgc gtagtattcc
    25681 acgcgcaccg cgccatgaga gtggatccct ctggcatcgg cgtaaaccaa tacttcagcc
    25741 acggttgcag cgtgctcacg ttttaaccca gcctggcaga gtttattctc aattagctgg
    25801 tggagtgttt cccgactgat tttcatctgt cttccttttt aacgacggtg tgaagcatga
    25861 ctgcaattaa catacaggga aaatatctgg attatgtgat ccagacaggc aaaaaaatat
    25921 agttagaatt tatttgataa tccgctcgct tttaacctgg tttttaaaac aaccacgctt
    25981 attaaaaaat aacacgtaat agcatggcgg ttatttgaat tcttttgtta ataatccctg
    26041 tgtgatattc atcaccttat ttacccgttg tcatcgatac cgtaatcgcc acattaacac
    26101 tgctcgtgca attgccatgg gtgcaatttt taaggagttg ttatgatcca cgcctttatt
    26161 aaaaaagggt gttttcagga ttcggtcagt ttaatgatta tttcacgaaa actaagcgaa
    26221 tcagaaaatg ttgatgatgt ttccgtaatg atgggtacgc ccgccaataa agcgttatta
    26281 gataccacag gtttctggca tgacgatttt aataacgcca cgccgaacga tatttgcgtg
    26341 gcaattcgta gcgaagcggc ggatgcgggg atcgcgcagg cgattatgca gcagcttgaa
    26401 gaggcgctaa aacaactggc gcaggggtca ggcagcagcc aggcgttgac gcaggtgcgt
    26461 cgctgggaca gtgcctgtca gaaattaccc gacgccagtc tggcgctgat ttcagtggct
    26521 ggcgagtatg cggcggagct ggcgaatcag gcgctggatc gcaacctcaa cgtgatgatg
    26581 ttctctgata acgtcacgct ggaagatgaa atccaactta aaacccgcgc gcgggaaaaa
    26641 ggcttgctgg tgatggggcc ggactgcggt acgtcgatga ttgccggcac gccgctggct
    26701 tttgctaacg tgataccgga aggcaacatt ggcgtcattg gcgcttccgg taccgggatt
    26761 caggaactgt gttcgcagat tgcgctggca ggtgagggaa ttactcacgc gattggcctt
    26821 ggcgggcgcg atctcagccg tgaagtgagc ggcatcagtg cgctaacggc gctggaaatg
    26881 ctcagtgcag acgagaaaag cgaagtgctg gcatttgttt caaaaccacc tgccgaagct
    26941 gtgcgtctga aaattgttaa tgccatgaaa gcaaccggca aaccgacggt ggcgctgttt
    27001 ttaggttata ccccggcggt ggcccgcgac gagaatgtct ggtttgcctc ctcgctggat
    27061 gaggccgcac gcctggcttg cctgctttca cgcgtcacgg cgcgacgtaa cgcaatagca
    27121 cctgtcagca gcggatttat ttgcggtttg tataccggcg gtacgctggc tgctgaagcg
    27181 gcgggattac ttgccggaca ccttggcgtg gaagccgacg atacccatca acatggcatg
    27241 atgctcgacg ccgacggaca tcagatcctc gacctgggcg atgattttta caccgtcggg
    27301 cgtccccatc cgatgatcga cccgacctta cgcaacctgt taattgccga tctcggcgct
    27361 aaaccgcaag tgcgcgtgtt gctgcttgat gtcgtgattg gcttcggtgc gactgccgat
    27421 cctgccgcct cgctggtgag cgcctggcaa aaagcctgtg ccgcgcgtcc agacaatcaa
    27481 ccactgtatg ccattgccac ggtgacaggc accgaacgtg acccgcaatg ccgctcgcaa
    27541 caaatcgcca cgctggaaga tgcggggatc gcggtcgtga gttcgctgcc ggaagccacc
    27601 ttgctggcgg cagcgttaat tcatccgctc tcgcctgcca cacagcaaca cacaccgtca
    27661 ttactggaaa acgtcgccgt gattaacatc ggattacgca gctttgcgct ggcgctacaa
    27721 agcgccagca aaccggttgt gcattaccaa tggtcgccag tcgccggcgg caataaaaaa
    27781 ctggctcgtt tattagaacg tttgcaataa ggggttccca tgtttacatc agtggcgcaa
    27841 gccaatgctg cggttatcga acaaattcgt cgcgctcgtc cacactggct ggatgtgcaa
    27901 ccggcttctt cacttatcag cgaactaaac gagggcaaaa cactgcttca cgccgggccg
    27961 ccaatgcgct ggcaggagat gaccggaccc atgaaagggg cgtgcgtggg cgcatgtctg
    28021 ttcgaaggct gggcgaaaga tgaagcgcag gcgctggcaa tactggagca gggggaagtg
    28081 aacttcattc cttgtcacca tgtgaatgcc gtagggccaa tgggcggtat tacttctgcc
    28141 agtatgccga tgctggtggt cgagaacgtg accgacggca accgggcgta ctgcaacctc
    28201 aacgaaggta tcggcaaagt gatgcgtttt ggcgcttacg gcgaagatgt cctgactcgc
    28261 catcgctgga tgcgcgatgt gttaatgcca gtattaagcg cggcgctggg gcgcatggat
    28321 cgcggtatcg atctcacggc aatgatggcg cagggcatta ccatgggcga tgagttccat
    28381 caacgcaata ttgcttcctc agcactgtta atgcgtacgc tggccccaca aattgctcgc
    28441 cttgatcatg ataaacagag catcgccgaa gtgatggatt tcctcagcgt gaccgatcag
    28501 ttcttcctta acctcgcgat ggcttactgc aaggcggcga tggatgctgg tgcgatgatc
    28561 cgcgcaggca gcatcgtcac ggcaatgacc cgcaacggca atatgttcgg gattcgggtc
    28621 agcgggctgg gcgaacgctg gtttactgcg cctgtaaaca ctccgcaagg tctgtttttc
    28681 accggcttct cgcaggatct ggcgaacccg gatatgggcg atagtgcgat taccgaaacc
    28741 tttggtatcg gaggtgcggc aatgatcgca gcgcctggcg taacgcgctt tgtcggtgcg
    28801 ggtggcatgg aagcggccag agcggtatct gaagagatgg cggaaattta ccttgaacgc
    28861 aatatgcagt tgcagatccc aggctgggat tttcagggcg cgtgcctggg gctggatatt
    28921 cgtcgcgtgg tagaaaccgg cattacgcca ctcatcaata ccggtatcgc ccataaagag
    28981 gcggggatcg ggcagattgg cgcaggcact gtgcgggcac cgttggcctg ctttgaacag
    29041 gcgctggaag cactggctga aagcatgggg ataggttgag gaacgcgcaa tgacgatcat
    29101 ccatcctctg cttgccagcc gtagcgcacc gaattattgc cagtcctggc ggttagcggg
    29161 agtgtggcga cgggcgatta acctgatgac ggaaagcggc gaactgttaa cgctgcatcg
    29221 tcagggtagt ggtttcggcc ccggaggatg ggtgcttcgc cgcgcgcaat tcgatgcgtt
    29281 atgtggtgga ttatgcggca atgaacgacc gcaggttgtg gctcaaggga ttcgcctcgg
    29341 gcgtttcacg gttaaacagc cacagcgtta ttgtttgctg cgtattacgc cgcctgcgca
    29401 tcctcaaccg cttgcagctg catggatgca acgcgcggag gaaaccgggc ttttcgggcc
    29461 actggcgttg gcggcaggcg atccgctgcc cgctgaatta cgccagtttc gtcactgttt
    29521 tcaggccgcg ctcaatggcg tgaagaccga ctggcggcac tggctgggta aaggtccggg
    29581 attaacgcca agtcatgatg acacgctgag cggaatgctg ctggcggcct ggtattatgg
    29641 cgctttagat acgcgctccg gtcgccagtt ttttgcctgt tccgacaatc ttcaactcgt
    29701 taccacagcg gtgagcgtta attatttacg ttatgccgcg caaggatatt tcgcctcgcc
    29761 actcctgcac tttgttcatg ctctgagttg cccgaaacgt accgctgttg cgattgattc
    29821 gctgatggcg ctggggcata cgtcaggggc agatacgctg ctggggttct ggcttggtca
    29881 acaattatta caaggaaaac catgaaaaca ctggttgtgg ctcttggggg caatgccttg
    29941 cttcagcgcg gtgaggcgct gacggcagaa aatcaatatc gcaatatcgc cagcgctgta
    30001 cccgcactgg cgcgcctggc ccgttcttat cggctggcga ttgttcacgg caacgggcca
    30061 caggtggggc tgttggcgtt acagaatctg gcgtggaaag aggtagaacc gtatccgctg
    30121 gatgtgctgg tggcggaaag ccaggggatg attggctata tgctggcgca gagcttgagc
    30181 gcacagccgc agatgccgcc tgtcacgacg gtgctgacgc gcattgaggt ttcgcctgat
    30241 gatccggcgt ttttgcagcc agagaagttt atcggtccgg tttatcagcc agaagaacaa
    30301 gaggcactgg aagcggctta cggctggcag atgaaacgtg atggtaaata tttgcgccgg
    30361 gtggtggcgt ctccgcaacc gcgtaaaatt ctcgacagcg aagccatcga gttgttgctc
    30421 aaagaggggc atgtggtgat ttgcagtggc ggcggcggtg tgcctgtgac ggatgacgga
    30481 gcagggagtg aagcagtgat tgataaagat ctcgccgctg cgttgctcgc cgagcagatt
    30541 aacgccgatg ggctggtgat cctcaccgat gctgatgcgg tatatgaaaa ctggggaacg
    30601 ccgcagcaac gtgccattcg ccatgccaca ccggatgagt tagcgccatt tgccaaagcc
    30661 gatggttcga tggggccgaa ggtaacggcg gtgagtggct atgtcagaag ccgtggcaaa
    30721 cctgcgtgga ttggggcgtt atcgcgaatt gaagagacac tggcgggcga agcggggacc
    30781 tgtatttcgc tgtagtgatg agagcatccg gcaatcggcg caccatgcct gatgcgatgc
    30841 ttgacgcgtc ttatcaggcc tacaatgggt accggagcgg taagccggat aaggcgttca
    30901 cgccgaatcc ggcaagaata tgagcaccag gttaattgaa cttactctgc gcccacatca
    30961 cgccgctggc atactctggc ggcagcagcg ggattaaggc ttccagcgtc gcggtcagac
    31021 gcgatgtgtc gctgtcggtc aaattcagat gccccacttt acgccccgga cggacttctt
    31081 tgtcgtacca gtgcagatgt accagcggca gtttcagcca gtcataattc acatcgctac
    31141 caatcaggtt gatcatcacc gacggactat tcactaccgg ttgcggtaac ggcagatcgg
    31201 taatcgcccg cagatgcatc tcaaactggc tgatgctggc accgttttgt gtccagtgac
    31261 cgctgttatg cacacgcgga gccagttcgt tgatcagcag accttgcggg gtgacaaaac
    31321 actccatcgc catcacgccc acatagccca gctcctgcat aatcgccgac agcatctctt
    31381 cggcctgcgc ctgctgctgc gcgttggcct gcgggaaagc gacgctggtg cgcaaaatac
    31441 cgtcctgatg caggttatgc gtcagcggat aaaacacggt gctaccatca aagccgcgcg
    31501 cgccaaccag cgacacttcg ccagagaagt taatgccctg ctcgacaata cattcgccgt
    31561 aacactctgc cggtaactgt tcggtttcat cggcacgtaa acgccattga ccgcgaccgt
    31621 catagccacc agtgcgacgc ttaacaatcg ccagctcacc taaacgatca aacaccgcag
    31681 gccactcgct gcgatcggca agtaactgcc acggtgcggt cggcaggtga agcttgtcga
    31741 aaagctgctt ctgagtcaga cggtcggcaa taatcgggaa cacatcgcgg ttcacaaatg
    31801 ccggatggcg cgccagctcg cgggttaatg cggtttccgg ccagcgttcg atctcagcgg
    31861 taatcacgct ttgttgaaaa ggcaccgccg ccggttcagc gtccagcccg accggccaga
    31921 cagcaatgcc taacggttcg cctgcctgac gcagcatacg gcctaactgc ccgttaccga
    31981 ggacgcaaac ctgtttcatg ccgcacctcg cgggtccggg ttttccagca cttcatcagt
    32041 ttgtgctttg cgccagtcat tcagacgctg gtgcaattct ttatcatgag tcgcaagaat
    32101 ttgtgctgcc agtaacgccg cgtttgccgc gccagcttta ccaatcgcca gtgtacccac
    32161 cggaatgccg cgcggcattt gtacgatgga gtagaggcta tcgacaccgc tcagtgcggc
    32221 gctctgtact ggcacgccca gcactggcac cagcgttttg gcggcaatca tgccaggcag
    32281 gtgcgctgcg ccgcctgcgc ccgcaataat cacctgataa ccgttctctt cggcactttc
    32341 ggcgaagctg aacagtttat cgggggtgcg gtgagcagaa accacttcaa cgtggtgcgg
    32401 gacattcagg atttcgaaga tttcggcggc gaactgcatg gtagcccagt cgcttttgga
    32461 ccccatcacg atggcgacac gcgccggatt attgcgggaa gacatgcgtc ttaaaactcc
    32521 tgtggtgcac aactctcggc tttagagggc acagagaata gcacggaaag agagcaagga
    32581 aaacggttgc gtggctgtga aatcagcaaa gttgcgggta tttaaaacgg aaaatgaatc
    32641 agctcaacgt catccgccgt gactttcacc attgaacctt ccgtatgcca ggcacccagt
    32701 accacgcgga aagcaggttg ctgattggcg ataagttcat gtactgccgg gcgatgggta
    32761 tgcccgtgaa tcagccattg cacctgatgt ttttccatcg cactgaccac cgcgttttgg
    32821 ttaacgtcca tgatcgccag cgatttactg ctgttggctt ctttgctgtt cgctcgcatt
    32881 cgcgcggcaa tgcgtttgcg cacaaacaac ggcagggcga ggaatagcgt ttgcagccag
    32941 ggtttgtgga ctttggcgcg aaaagcctga taaccggcgt catcggtgca cagcgtgtcg
    33001 ccatgcataa tcaacacctg gcgaccatac agttcgagca ccttttcttc cggcaataac
    33061 gtcatgccgc tttcacgggc aaagcgtttg ccgagcagaa aatcacggtt gccatgaatg
    33121 aaataacagg gaacgccgga atcggacacc gctttgatcg ctgccgccat ctggcgatgg
    33181 agtgggttgg gatcgtcgtc gccaatccac gcttcaaaca gatcgccaag aatatacagc
    33241 gcgtcggcct tgcgggcttc ccccgctaaa aaacgcagaa aaccggcggt gatcgccggt
    33301 tcttccgcgc agagatgaag atctgcaata aagagtgtcg ccacgattac tcgctaacgg
    33361 tcacgctttc aatgataacg tcttcttttg gcacgtcctg gtgcataccg ctacgaccgg
    33421 ttgccacacc tttgattttg tctaccacgt ccatgccttc aaccacttca gcaaacacgc
    33481 agtaacccca accttgcagg ctttcgccag agaagttcag gaagtcgtta tcaaccacgt
    33541 tgatgaagaa ctgtgcagtt gcagagtgcg gtgcctgagt acgggccatt gccagtgtac
    33601 cacgggtatt tttcagaccg ttgttggctt cgtttttgat cggttctttg gtggcttttt
    33661 gtttcatgcc cggttcaaaa ccgccgccct gaatcataaa gccgttgata acacggtgga
    33721 aaatggtgtt gttgtaaaaa ccttcgcggc agtagtccag gaagttttta actgtttcag
    33781 gtgctttatc gtcaaaagtt ttgatgacaa tatcgccgtg attggtgtgg aaagtaacca
    33841 tttttgcatc ctgttccgtt tgattggtgc ttcaacccgg ttcgggtcat atatagggtg
    33901 gtgttatagc ataaccgcac gatcggatca tctcgcaatg tatgctgatt cgcgcgggaa
    33961 atatgggtat tatacgcaac tcaattaccc acacatgtct aaacggaatc ttcgatgcta
    34021 aaaatcttca atactctgac acgccaaaaa gaggaattta agcctattca cgccggggaa
    34081 gtcggcatgt acgtgtgtgg aatcaccgtt tacgatctct gtcatatcgg tcacgggcgt
    34141 acctttgttg cctttgacgt ggtcgcgcgc tatctgcgtt tcctcggcta taagctgaag
    34201 tatgtgcgca acattactga tatcgacgac aaaatcatca aacgcgccaa tgaaaatggc
    34261 gaaagctttg tggcgctggt ggatcgcatg atcgccgaaa tgcacaaaga ttttgatgct
    34321 ttgaacattc tgcgcccgga tatggagccg cgtgcgacgc accatatcgc cgaaattatt
    34381 gaactcactg aacaactgat cgccaaaggt cacgcttatg tggcggacaa cggcgacgtg
    34441 atgttcgacg tcccgaccga tccgacatat ggcgtgctgt cgcgtcagga tctcgaccag
    34501 ctgcaggcag gcgcgcgcgt tgacgtggtc gacgacaaac gcaacccgat ggatttcgtt
    34561 ctgtggaaga tgtcgaaaga gggcgaaccg agctggccgt ctccgtgggg cgcgggccgt
    34621 cctggctggc acattgaatg ttcggcaatg aactgcaagc agctgggtaa ccactttgat
    34681 atccacggcg gcggttcaga cctgatgttc ccgcaccacg aaaacgaaat cgcgcagtcc
    34741 acctgtgccc atgatggtca gtatgtgaat tactggatgc actccgggat ggtgatggtt
    34801 gaccgcgaga agatgtccaa atcgctgggt aacttcttta ccgtgcgcga tgtgctgaaa
    34861 tactacgatg ctgaaaccgt gcgttacttc ctgatgtcgg gccactatcg cagccagctg
    34921 aactacagcg aagagaacct gaagcaggcg cgtgcggcgc tggagcgtct ctacactgcg
    34981 ctgcgcggca ccgataaaac cgttgcgcct gcgggtggtg aagcgtttga agcgcgcttt
    35041 attgaagcga tggacgacga tttcaacacc ccggaagcct attccgtact gtttgatatg
    35101 gcgcgtgaag tgaaccgtct gaaagcagaa gatatggcag cggcgaatgc aatggcatct
    35161 cacctgcgta aactttccgc tgtattgggc ctgctggagc aagaaccgga agcgttcctg
    35221 caaagcggcg cgcaggcaga cgacagcgaa gtggctgaga ttgaagcgtt aattcaacag
    35281 cgtctggatg cccgtaaagc gaaagactgg gcggcggcag atgcggcgcg tgaccgtctg
    35341 aatgagatgg ggatcgtgct ggaagatggc ccgcaaggga ccacctggcg tcgtaagtaa
    35401 ttgcgctatt gccggatgcg agttttcgca tccggttatc gcctgcgcca ccacaacatt
    35461 cccatcagta gcatccccgg caaccacacc cacattaatt cagaaataat cacctgatgc
    35521 ccgtacggcg tggtgtaacg agacaatgca aacggcgcga ctttaatcac ctgccagggg
    35581 gcgaaaaagc gttcatctga ccacggccac agccagccaa cccctttacc accagtggtt
    35641 acagaatcca gcaagctgtg cgatagcaac gagacggtta aaaacaccca gcagcgaatc
    35701 agcccagccc tgaaccatcg gcgtccaatc agcacacata acagcgggac aacaaacgca
    35761 aacaccagcg aatgggtaaa cccgcgatga ccaaaaacat tgccataagc aacgccaaat
    35821 ttaaacgaca acacgtcggc atcgggcagc atcgccagga tgattccggc aaataacaga
    35881 cgcggaggga tgactttcga acccaaccct aaagcaatgc aaaggggaac ggcggcgtgc
    35941 gtaacaacgg ttggcatgat ggtcgcttcg gcaaaatgtc gatgctatca gcatggatga
    36001 acggggcgta gagggcaaaa gtctgaaaag agaaccggcc tgttgttaca ggccgggaaa
    36061 gggatcaggc aacaacctgt acgctgtgac cggcaaaact cactgtctgc ccggcaacga
    36121 ttttgcagcg tttgcgcgtt tcaaccgcac cgtcgacttt cacctggcct tcggcaatcg
    36181 cgattttcgc ctgcgcgccg ctttcgctcc agccttccag tttcagcaag tcgcacagct
    36241 caacgtgcgg atgtttacct aaagaaaatg tcgccatgtt actcaccctg tggatcatga
    36301 tattcaacgc acgcctgtag cgtgttttca atcagcgtgg caaccgtcat cgggccaacg
    36361 ccgccgggaa caggcgtaat gtatgaggcg cgtttagccg cgtcttcaaa cacgacgtcg
    36421 cccacaactt tgccattttc cagacggttg atgccgacat caatcacaat tgcgccttct
    36481 ttgatccagt caccgggaat aaagcctggc ttaccgacgg caacgatcaa tagatcggca
    36541 ttttctacgt ggtgacgcag atttttagtg aaacggtgag tcacggtggt ggtgcaacct
    36601 gccagtagca gttccatgct catcgggcgg ccaacgatat tcgatgcgcc aatcaccacg
    36661 gcgttgaggc cgaaggtatc aatgttgtaa cgctcaagca gcgtgacgat accgcgcggg
    36721 gtgcagggac gcagacgcgg cgcgcgctgg cacagacgac cgacgttgta aggatggaaa
    36781 ccgtccacgt ctttgtccgg atgaatacgt tccagcactt tgacgttatc aatacccgcc
    36841 ggtaacggca gttgaaccag aatgccatca atggtgttgt cggcattcag cgcatcgata
    36901 agctccagca gctccgcttc gctggtggtt tccgggaggt cataagagcg ggagacgaac
    36961 cccacctctt cacaagcctt gcgtttgctt gcgacataaa tttgcgatgc agggttacta
    37021 cccaccagca caacggccag tcctggtgcc cgcagtccgg ctgcaatacg cgcctgaact
    37081 ttttgagcaa cttcagagcg cacctgctgc gcaatcgttt taccgtcaat aatctttgct
    37141 gccatcagag agaggattcc atctgttacg taggtcgaag gggatgtgcc tattttgtca
    37201 gaagcggggc gcgctgtcag gtttcgtttc agatttatcg cgtgaagcga cctcttgcga
    37261 aggtgatgcg caccgtcgct gagattgaaa gcttcatttt tcgtccatga tggcgttgta
    37321 aatctggaac tgacttattt ccttgtcaaa ggattaagat aatttaagaa atacctgaca
    37381 atataaaaag aattttcagc ctggtaattt accgct
//
LOCUS       DARNVM010000025        37113 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.025, whole genome shotgun sequence.
ACCESSION   DARNVM010000025 DARNVM010000000
VERSION     DARNVM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.025"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            103..1761
                     /locus_tag="SI621_001221"
     CDS             103..1761
                     /locus_tag="SI621_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEI4063961.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1758..2714)
                     /locus_tag="SI621_001222"
     CDS             complement(1758..2714)
                     /locus_tag="SI621_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEI4063962.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2865..3980
                     /gene="macA"
                     /locus_tag="SI621_001223"
     CDS             2865..3980
                     /gene="macA"
                     /locus_tag="SI621_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HEI4063963.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKSGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3977..5923
                     /gene="macB"
                     /locus_tag="SI621_001224"
     CDS             3977..5923
                     /gene="macB"
                     /locus_tag="SI621_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HEI4063964.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5996..6220)
                     /gene="cspD"
                     /locus_tag="SI621_001225"
     CDS             complement(5996..6220)
                     /gene="cspD"
                     /locus_tag="SI621_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HEI4063965.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6543..6863
                     /gene="clpS"
                     /locus_tag="SI621_001226"
     CDS             6543..6863
                     /gene="clpS"
                     /locus_tag="SI621_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEI4063966.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6894..9170
                     /gene="clpA"
                     /locus_tag="SI621_001227"
     CDS             6894..9170
                     /gene="clpA"
                     /locus_tag="SI621_001227"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEI4063967.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9513..9647)
                     /locus_tag="SI621_001228"
     ncRNA           complement(9513..9647)
                     /ncRNA_class="other"
                     /locus_tag="SI621_001228"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(9871..9958)
                     /locus_tag="SI621_001229"
     tRNA            complement(9871..9958)
                     /locus_tag="SI621_001229"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9922..9924),aa:Ser,seq:gga)
     gene            complement(10212..10430)
                     /gene="infA"
                     /locus_tag="SI621_001230"
     CDS             complement(10212..10430)
                     /gene="infA"
                     /locus_tag="SI621_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEI4063968.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10715..11419)
                     /gene="aat"
                     /locus_tag="SI621_001231"
     CDS             complement(10715..11419)
                     /gene="aat"
                     /locus_tag="SI621_001231"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEI4063969.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11461..13182)
                     /gene="cydC"
                     /locus_tag="SI621_001232"
     CDS             complement(11461..13182)
                     /gene="cydC"
                     /locus_tag="SI621_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HEI4063970.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13183..14949)
                     /gene="cydD"
                     /locus_tag="SI621_001233"
     CDS             complement(13183..14949)
                     /gene="cydD"
                     /locus_tag="SI621_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HEI4063971.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFSTLLAHRQEEI"
     gene            complement(15072..16037)
                     /gene="trxB"
                     /locus_tag="SI621_001234"
     CDS             complement(15072..16037)
                     /gene="trxB"
                     /locus_tag="SI621_001234"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEI4063972.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16582..17076
                     /gene="lrp"
                     /locus_tag="SI621_001235"
     CDS             16582..17076
                     /gene="lrp"
                     /locus_tag="SI621_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HEI4063973.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17211..21278
                     /gene="ftsK"
                     /locus_tag="SI621_001236"
     CDS             17211..21278
                     /gene="ftsK"
                     /locus_tag="SI621_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEI4063974.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAQEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQSAQQSYYAPA
                     PEQSVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPVAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKTPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21437..22048
                     /gene="lolA"
                     /locus_tag="SI621_001237"
     CDS             21437..22048
                     /gene="lolA"
                     /locus_tag="SI621_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEI4063975.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22059..23402
                     /gene="rarA"
                     /locus_tag="SI621_001238"
                     /gene_synonym="mgsA"
     CDS             22059..23402
                     /gene="rarA"
                     /locus_tag="SI621_001238"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HEI4063976.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23493..24785
                     /gene="serS"
                     /locus_tag="SI621_001239"
     CDS             23493..24785
                     /gene="serS"
                     /locus_tag="SI621_001239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEI4063977.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25024..27468
                     /gene="dmsA"
                     /locus_tag="SI621_001240"
     CDS             25024..27468
                     /gene="dmsA"
                     /locus_tag="SI621_001240"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HEI4063978.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENMVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27479..28096
                     /gene="dmsB"
                     /locus_tag="SI621_001241"
     CDS             27479..28096
                     /gene="dmsB"
                     /locus_tag="SI621_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HEI4063979.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28098..28961
                     /gene="dmsC"
                     /locus_tag="SI621_001242"
     CDS             28098..28961
                     /gene="dmsC"
                     /locus_tag="SI621_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HEI4063980.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28996..29622)
                     /locus_tag="SI621_001243"
     CDS             complement(28996..29622)
                     /locus_tag="SI621_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEI4063981.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29937..31085
                     /locus_tag="SI621_001244"
     CDS             29937..31085
                     /locus_tag="SI621_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4063982.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31295..32725
                     /locus_tag="SI621_001245"
     CDS             31295..32725
                     /locus_tag="SI621_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEI4063983.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32726..32836)
                     /locus_tag="SI621_001246"
                     /pseudo
     CDS             complement(32726..32836)
                     /locus_tag="SI621_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(32935..33675)
                     /gene="pflA"
                     /locus_tag="SI621_001247"
     CDS             complement(32935..33675)
                     /gene="pflA"
                     /locus_tag="SI621_001247"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HEI4063984.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(33867..36149)
                     /gene="pflB"
                     /locus_tag="SI621_001248"
     CDS             complement(33867..36149)
                     /gene="pflB"
                     /locus_tag="SI621_001248"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEI4063985.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36204..37061)
                     /gene="focA"
                     /locus_tag="SI621_001249"
     CDS             complement(36204..37061)
                     /gene="focA"
                     /locus_tag="SI621_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HEI4063986.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9074 a         9073 c         9934 g         9032 t
ORIGIN      
        1 cagacatttg ccatgcttaa atgtgatgtc atcacgtatt agcatggcct ttcccgttat
       61 actgccagcg taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt
      121 gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga tgctggaaca aaacaacgtc
      181 ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta
      241 tcgccagaat cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat
      301 atcaacgggc gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc
      361 cgacatcggg ttcgccgcta tcggccgctg gaagcgtgct ggacgccatg caccgatggc
      421 tatcaccgta ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca
      481 ctgcgcagtt ttctcgataa agacggacat ccgattgatg tcgaggatat taacgaccag
      541 gcacgccatc tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc
      601 cgtattcgta acggcacggt gccaaatgtc cctaatgtgg aagtcaccgc gcgccagctc
      661 gatttcctcg cccgtgagtt atcctcacat ccgcaaaatc tctctgatgg gcagattcgt
      721 cagggacttt ccgcaatggt gcaactgctt gagcattatt tctctgagca gggggccgga
      781 caggcgcgat atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat
      841 ctggatatca tcaaccggat gattgaccga cctggtgggc gctcgtatcg ggttattttg
      901 ctcggcctgt ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcc
      961 cgtccattgt tgctgatcga agatccagaa acccgtttac accccattat gctttcagtt
     1021 gcctggcatc tgttgaatct tctgccattg cagcgcattg ccaccaccaa ctcgggtgag
     1081 ttgctttcgt taacgccggt agagcatgtt tgccgactgg tacgtgagtc ctcgcgcgtt
     1141 gccgcctggc gtctggggcc gagtggcttg agtaccgaag atagccgacg catatccttt
     1201 cacattcgtt ttaaccgtcc gtcatcgctg tttgcacgct gctggctgct ggtggaaggg
     1261 gaaacggaaa cttgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc
     1321 gaagggatca aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc
     1381 cggcgaatgg ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat
     1441 gccgctacgg tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg
     1501 gcgttaccgg cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc
     1561 caccgcgtgg cgcaaatccc ggaaaatgta ccgatgaatc tgcgcaaaat tatctcgaaa
     1621 gcgatccatc gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt
     1681 cgtggtgtgg attccgtacc gacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg
     1741 gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg
     1801 agcatctcat agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct
     1861 tttcgtgcta tctgtgccgg aaggcggtaa tggcgttcag catcacatac gccgccaacc
     1921 gactcccaga aagcgttgta atcggcatgg atcttgcctt ctttatcgcg ataacgcagg
     1981 ctgcggtaaa tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg
     2041 gcaaacagac aggctgcttc catcacaagg cgtttgggaa atagcccgtg gcaggctttc
     2101 gtcgcattct ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata
     2161 aacattgttc ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata
     2221 ccttcgctgt tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg
     2281 gtcagctcaa gtgtgaactg ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg
     2341 ggggtattca aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa
     2401 tggtaacgta acgcctccag caattgctta cggctaagat tcgccgcaag gtaagggcga
     2461 tgcagacgca caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg
     2521 gaaagttcgt tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat
     2581 ttctggcgaa aactacggcg atgccagaat ttacccggcc gacactgtcc acgtgccaga
     2641 ctaagaaaaa gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc
     2701 tgcgacatat tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt
     2761 aaccaaacat caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt
     2821 gaagttaatg acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa
     2881 ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat tgccgggctg attacgttat
     2941 ggagaattct taacgcaccc gtgccgactt atcagacgct gattgtgcgc cccggtgatt
     3001 tacagcaaag cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gatgtgggcg
     3061 cgcaggtcag cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag
     3121 accagctttt aggggttatt gatcctgaac aggctgaaaa ccagattaag gaggtggaag
     3181 caacgctgat ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc
     3241 gggtgacgta ttcccgtcag caacgtcttg cacaaacgca agcggtttca cagcaggatc
     3301 ttgataccgc cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc
     3361 aaatcaagcg caatcaggcc tcactcgata cggctaaaac caatctcgat tacacccgca
     3421 tcgttgcccc gatggccggg gaagtcacgc aaatcaccac tctgcaaggc cagacggtga
     3481 ttgccgcaca acaagcaccg aacattctga cgctggcaga tatgagcacc atgctggtaa
     3541 aagcgcaggt ttctgaagcg gatgtaatcc acctgaagtc ggggcaaaaa gcctggttta
     3601 cggtgcttgg cgatccgctg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc
     3661 cggaaaaggt taacgacgct attttctatt acgcccgttt tgaagtcccc aaccccaatg
     3721 gtttgctgcg gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg
     3781 tgctgacgat ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca
     3841 aattgttgcg taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata
     3901 ccgatgttga gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca
     3961 aaccaggagc tgcacaatga cgcccttgct cgaattaaag gatattcgtc gcagctatcc
     4021 tgccggtgat gagcaggttg aggtgctgaa gggcatcacc ctcgatattt atgctggtga
     4081 gatggtggcg attgtcggcg cttcgggttc cggtaaatcg accctgatga atattctcgg
     4141 ctgcctggat aaggccacca gcggcaccta tcgcgtagcc ggtcaggatg ttgccacgct
     4201 ggacgccgat gcgctggcgc aactgcgtcg cgaacacttc ggctttattt tccagcgtta
     4261 ccatttgctt tcgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg
     4321 tcttgagcgg aaacagcggc tgcttcgcgc ccaggagtta ctgcaacgac tggggctgga
     4381 agaccgtaca gagtattacc cggcacagct ttcggggggg cagcaacagc gcgtcagcat
     4441 cgcgcgggca ttgatgaacg gcggtcaggt aattcttgcc gatgaaccaa ccggcgcact
     4501 ggacagccat tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca
     4561 tacggtgatt atcgttaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga
     4621 aattcgcgac ggcgaaattg tgcgcaatcc tcccgccgtt gaaaaagtga atgttgctgg
     4681 cgggacggaa cctgttgtca acacggcctc tggctggcgg caatttgtca gcggttttaa
     4741 cgaagcgctg acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac
     4801 catgctgggg attattatcg gtattgcgtc ggtggtttca attgtcgtgg tgggcgacgc
     4861 cgccaaacaa atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta
     4921 tcccgggaaa gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga
     4981 cttaatcgcc atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa
     5041 cctgcgcctg cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga
     5101 ttattttaat gtctatggca tgaccttcag tgaagggaac acctttaatc aggagcagct
     5161 gaacggtcgt gcgcaggtgg tggtgctcga cagtaatact cgccgccagc ttttccccca
     5221 taaagcagat gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg
     5281 tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc
     5341 ttacagcacg atgtccgggc gagttatggg ccagtcgtgg ctgaactcca ttactgtcag
     5401 ggtgaaagaa ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact
     5461 gcgccacggt aagaaggatt tctttacctg gaacatggac ggcgtcttga aaaccgttga
     5521 aaagaccaca cgtactttac aactgtttct gacgctggtg gcggtgattt cgctagtggt
     5581 gggcggtatt ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat
     5641 tggcattcgc atggctgtag gtgcgcgagc aagtgatgtg ttgcaacagt tcctgatcga
     5701 agcagtactg gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc
     5761 tttcacctta cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct
     5821 gctggcgttt ctctgctcga cggtcaccgg gattttattt ggctggttac ccgcacgaaa
     5881 tgcggcacga ctggatccag tagatgctct ggcacgagag taatttttga gataaaaatg
     5941 ccagccgatc gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg
     6001 cgactgccgc ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct
     6061 ggtggacatc aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa
     6121 tggtggaata atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc
     6181 ctttggcatt gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa
     6241 tcttatacaa gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc
     6301 aactctagaa atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag
     6361 ggggcaggta tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat
     6421 gtcgctgatg ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa
     6481 ataagctctt gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga
     6541 caatgggtaa aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg
     6601 acgcgctaaa accgccatct atgtataaag tgatattagt caatgatgat tacactccga
     6661 tggagtttgt tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc
     6721 aattgatgct cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg
     6781 ttgcagaaac caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc
     6841 tgtgtacgct agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca
     6901 atcaagaact ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg
     6961 agtttatgac cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg
     7021 cgctggaagc gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg
     7081 aacaaaccac acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga
     7141 gttttcagcg tgtgctgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg
     7201 ttaccggtgc aaacgttctg gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc
     7261 tgttgcgtaa acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc
     7321 gtaaagacga gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag
     7381 ctggtgggga ggaacgtatg gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg
     7441 gcggaatcga cccactgatt ggtcgtgaga aggagctgga gcgtgctatt caggttctct
     7501 gccgtcgccg taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga
     7561 ttgcggaagg tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact
     7621 gtacgattta ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact
     7681 ttgaaaaacg ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt
     7741 ttattgatga gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg
     7801 cggctaacct gatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa
     7861 cctatcagga gttcagcaac attttcgaga aagaccgtgc tttggcgcgt cgcttccaga
     7921 aaattgatat tactgaaccg tcgatcgaag aaactgttca aatcatcaac ggcctgaaac
     7981 cgaagtatga agcgcaccac gatgtgcgtt ataccgcaaa agcggtgcgt gcagcggtag
     8041 agctggcggt gaaatacatt aacgatcgtc atctgccgga taaagccatt gacgtcatcg
     8101 acgaagcggg cgctcgcgca cgcctgatgc cggtaagcaa acgcaagaaa accgttaatg
     8161 tggcggatat tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc
     8221 agagtgaccg cgataccctg aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggtc
     8281 aggataaagc cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc
     8341 acgaacataa accggtcggt tcgttcctgt ttgccggccc taccggggtc gggaaaacag
     8401 aggtgacggt acagctttcg aaagctttgg gcattgagct tctgcgcttt gatatgtccg
     8461 agtatatgga acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt
     8521 ttgatcaggg cggtttgctg actgatgcgg tcatcaagca tccacatgcg gtgctgctgc
     8581 tggacgaaat cgagaaagcg cacccggacg tgttcaatat tctgttgcag gtgatggaca
     8641 acggtacgct gaccgataac aacggacgca aagcggactt ccgtaacgtg gtattggtga
     8701 tgaccaccaa cgctggggta cgagaaactg agcgcaaatc gattggtctt atccaccagg
     8761 acaacagcac cgatgcgatg gaagagatca agaagatctt tacgccggag tttcgtaacc
     8821 gtctcgacaa cattatctgg ttcgatcatc tctccaccga cgtgatccat caggtagtgg
     8881 ataaattcat cgtcgagttg caggttcagt tggatcagaa aggtgtttct ctggaagtga
     8941 gccaggaagc gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc
     9001 cgatggcgcg tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgttgtttg
     9061 gttcgctggt ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga
     9121 cttatggatt ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc
     9181 aggtaggttg gtgaagtccg taatctcgaa agaggttacg gactttttgt ttgtaggctg
     9241 gggcggtgaa aaccctattt ttggaggtga aggtaagttg ttgataatta atggtgctgg
     9301 aaggtaagaa taaaaaaggg tggcagcagg aaaatgagat ggttttgctt tattaataac
     9361 ggactaaacg tgtagtattt gagttcactg ccgtacaggc agcttagaaa gccaaccgaa
     9421 agaaaaactc gatttttata ccgttcactg ccgtacaggc agataaaatg cgaaaaaaaa
     9481 ctcgtacttt cgtacgagct tttctttaaa tatggcggtg aggggggatt gactcgcttc
     9541 gctcgccctg cgggcagccc actcactgtg ttcgtggtct gtccaactgg ctgcgccagt
     9601 tgtcgaaccc cggtcggggc ttctcatccc ccctggagtg caatatgcga aaaaaaagct
     9661 cgcactttcg tacgagctct tctttgaata tggcggtgag ggggggattg actcgcttcg
     9721 ctcgccctgc gggcagccca ctcactgtgt tcgtggtctg tccaactggc tgcgccagtt
     9781 gtcgaacccc ggtcggggct tctcatcccc cctggagtgc aatatgcgaa aaaaaaagct
     9841 cgcactttcg tacgagcttt tctttaaata tggcggtgag ggggggattc gaacccccga
     9901 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
     9961 attgttttgc taccaaacct catgggtggc aacggggcgc tactataggg agttggagta
    10021 aaacggtcaa gaagaatttt aatgataatt attgtttgct catactgtaa acaagttgtg
    10081 cagtatatct acatcgagac aagttatgga cttatacttc caaagtactt catacatatc
    10141 acaaaataaa aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg
    10201 cggtaaaaca atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc
    10261 agttcaacag tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg
    10321 gagatgtgtg cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc
    10381 aacgtttcaa gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct
    10441 ggggtatcac taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa
    10501 agatttgcgc gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct
    10561 aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg
    10621 acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat
    10681 tataacactc tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcacc
    10741 gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattgaggt
    10801 aatcccggcg gggaatttcg caggcaccaa gcgacgctgt gtgatcgtta agtacctggc
    10861 agtcgataag cttaccgcca tgaccaataa attcctcaca gaataccaga agcgccgttt
    10921 tagacgcatt ttccatccgg ctgaacatgg actcaccaca aaatagcgtt ccctgggcca
    10981 cgccgtacat accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatgac
    11041 cgagttcgtg aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt
    11101 cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat
    11161 agggcgagcg tttatgaaat cgcttcatac tacggctgat atgcagtgat tcaggccaaa
    11221 gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc gccgggagaa aaccacggaa
    11281 aaataccacg ctggtaagcc attaacaggc gtgcagggct aagatcgccc ccaagtgcca
    11341 gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag
    11401 aaagctgaac caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc
    11461 ttacaaaccc tgcttaaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt
    11521 accttgctca ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc
    11581 gcgaagtcga tgggtgacca ttaacaccgt tttctcatgc atcatttctg caagcaattc
    11641 aaggatctga ctttcggttg tggcatctaa gccttctgta ggttcatcca gcaataccag
    11701 cggcgcatca tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggagag
    11761 ctggcgtccg ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag
    11821 accaacgcga cgtaagatct ccgccagagc ctcatcacta ctgccaggcg aggcgagtaa
    11881 cagattatca cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt
    11941 ctgtcgtaga gtcgcttcat tcaggctggc tatggggcta ccgttaagca aaatctcgcc
    12001 ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt
    12061 tcgcccgaga atcgctatat gttctccggc gtttacctga agagaaatcc ctttaagtgc
    12121 ctgttgggat tgctccggat aagtgaactg aacatcccgt aacgtcaacg aaacgcgatc
    12181 ggcaacacga gtttgggtat cagggaaggt gacttccggt ttttgatccg ttaagtcagt
    12241 gatccgaacg gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc
    12301 cagtgcttca aacgcggcta acgcgcagaa gacaaacagg gcaattaacg cgccgggttg
    12361 agcattgccg ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc
    12421 aatgagcagc attatcgctt gcgacaatgc ggtgagttca gattgacgtc gttgcgcttc
    12481 cagccactga atttcggtat tctctaactg cgtgcgataa cgatcgctgg caccaaaaat
    12541 ggtcagctca gcttgccctt gcagccaggc cgtcagttgt tggcgatact gcccgcgaag
    12601 atgagtcaga ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa
    12661 aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa
    12721 cccgattgtc accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag
    12781 gtaaagatga tcgagcgtat caacatccgc caccacgcgg ttgagcaatt cgccctgacg
    12841 atagcgcgcc agtccggcag gggagagggg cagcaatttg ctgaaggtat aaatgcgcag
    12901 atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat agcgcccggc
    12961 agtacgggtg attgctgcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta
    13021 cagtccggca acccccgcta ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag
    13081 accgatactg gcgagcagcg tgacaattgc cagcacaata ccaagactta acatccattt
    13141 atgacgttta tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg
    13201 atgggccagc aatgtggaga aaggaccacc agccacgctt aattccgcgt aacgtccttg
    13261 ctcaataatc tgaccatcct gcattaccca aatgacatcc cagtcagcaa gatcttctaa
    13321 ctggtgggtg accattaacg ttgtctggcg cagagaggcg gcattaagcg cctccattac
    13381 gcgctgttca ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca
    13441 gggatttagt aatgctcggg ccaccgccac gcgctgtgcc tgccccacgg aaaggcgggc
    13501 agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg agcggtagaa actcactgac
    13561 ccaggcgtta tccagcgctg tttgtaactc gtgttcgctg gcatcaggtc gtgccagtag
    13621 tacgttatcc cgtagtgttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg
    13681 tttacgccat gattccggtg ataaatcgcg taattctatc ccgttgattc gtagcgatcc
    13741 ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg
    13801 accaaccaac acggcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt
    13861 tttaccttct ggtgatgtaa taaacagctc ctcggcttca atggtcaccg gatcggtcga
    13921 tgctaattcc gcctcaccac gctgcggatg ggcaagcggg gtttccataa acgttttcaa
    13981 actgtcagct gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa
    14041 tggctggaaa aactccggcg caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc
    14101 agtatcatag tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac
    14161 cagagcaatt gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagccg
    14221 tagcacttcc attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc
    14281 agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgagaaaatg
    14341 cccacttaag cgagcaagag cgagaaagtt acgtcggtta gcatcggcag cccccattcc
    14401 aaccagcgcc ataaacaacg gaattaacgg agcagtgccc agcagaatga gcgccgcagc
    14461 ccagttagag gggaagatag ccaccacaat cagcaacggc accgacactg ccagcgccat
    14521 ttgcggtagg tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt
    14581 cgcccagctc cccgccggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc
    14641 gagaacctga cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc
    14701 gcgtaaccag accacccatg cgcgaagtac aaaggtcaga accagtaacg taaagggaag
    14761 cagcagggct tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa
    14821 ccaggcctgg gcaatgatca atatgccgct cacaaagccc agcagacgag aaatattcag
    14881 ccaacgttgg gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacggga
    14941 tttattcatt gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa
    15001 cgaggaaaaa ataaaggcga cccatagtcg catggtgtcg ccttctttac ttttgttact
    15061 gatttgtaaa attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct
    15121 gccatgcagc ctgtaccagc agatgtaata gcctggcgat aaatgtgatc catcacgtcg
    15181 cctgcggcaa agacgccagg gatgctggtc tgggtggcat taccatgaat acccgactgt
    15241 actttgatgt agccgttttc cagttccagc tgcccttcga aaatggcagt attcgggctg
    15301 tgaccgatag caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc
    15361 gtatcgcgca gacgaacgcc agtgacgccc atctgatcgc cggtcacctc ttccagcgta
    15421 cggttggtgt gtagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg
    15481 attttttccg cgcggaaacc gtcacggcgg tgaatcagat gaacttccga agcgatgtta
    15541 gacagataca gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg
    15601 ttgcgataga agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaacgct
    15661 tcctcagaag gaaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg
    15721 caagtgtatt cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg
    15781 ttgatatgat caaaaatgat ctcagtttca aatttggtgg catgttcgtg catgcgctcc
    15841 attaataacg gaccggtcag atcgtttgga tcgccaggcc agttttccac ttccgtggtg
    15901 gtggtcagtt ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc
    15961 gccgcataga cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg
    16021 tgtttggtcg tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg
    16081 gaatttacag acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag
    16141 catcgataag caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct
    16201 ttataagccg attaaatgat gaataaacgc ccctagtaat gaatatctgg catgttgtac
    16261 taaaaatcga tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga
    16321 aaaaaaacag tattcttata tgcgcataac catgcatgta aataccatgt ttaccgtgct
    16381 agtgaaatct acgtatggcg tggacagacg ccattcgtga tgtcgatagc agccacaagg
    16441 caacggtctt ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat gatttcggtc
    16501 tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg gtcagacagg agtagggaag
    16561 gaatacagag agacaataat aatggtagat agcaagaagc gccctggcaa agatctcgac
    16621 cgtatcgatc gtaacattct taatgagttg caaaaggatg ggcgtatttc taacgtcgag
    16681 ctttctaaac gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa
    16741 agacaagggt ttattcaggg ctatacggcg ctgcttaacc cccattatct ggatgcatca
    16801 cttctggtat tcgttgagat tactctgaat cgtggcgcac cggatgtgtt tgaacaattc
    16861 aataccgctg tacaaaaact tgaagaaatt caggagtgtc atttagtatc tggtgatttc
    16921 gactacctgt tgaaaacacg cgtgccggat atgtcagctt accgtaagtt gctgggggaa
    16981 accctgctgc gtctgcctgg cgtcaatgac acacggacat acgttgttat ggaagaagtc
    17041 aagcagagta atcgtctggt tattaagacg cgctaacacg gaacaggtgc aaaatcggcg
    17101 tattttgatt acactcctgt taatccatac agcaacagta ctggggtaac ctggtactgt
    17161 tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg agagcctttc ttgagccagg
    17221 aatacactga agacaaagaa gtcacattga caaagttaag tagcggccgc cgccttctgg
    17281 aggcgttgct gatccttatt gtcctgtttg ccgtctggtt gatggctgcc ttactaagct
    17341 ttaacccttc ggaccccagc tggtcgcaaa cggcctggca tgaacctatc cataatttag
    17401 gtgggatgcc cggtgcatgg ctggcagata cgctgttctt tatttttggc gtgatggctt
    17461 acaccattcc cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat cagtccagcg
    17521 acgaatacat tgattatttt gccgtttcgc tacgcatcat tggcgttttg gcgctcatcc
    17581 ttacctcctg tggtctggcg gcaatcaacg ctgacgatat ctggtatttt gcctccggtg
    17641 gcgtcattgg cagcttacta agcactacgc tacaaccact gctacacagt agcgggggaa
    17701 ctattgcgct gctctgcgtt tgggctgcgg gtctgacgct gttcaccggt tggtcatggg
    17761 tgaccattgc tgaaaaactc ggcggctgga ttttaaacat tctcaccttc gccagtaatc
    17821 gtacccgtcg cgatgatacc tgggtcgatg aagatgagta tgaagacgac gaagagtatg
    17881 aagatgaaaa tcacggcaaa cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgt
    17941 tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc gatggggcgg caaacagatg
    18001 ctgcgttgtt ctccggtaag cggatggatg atgaagaaga gattacctac actgcacgcg
    18061 gtgtggctgc cgacccggac gacgtcctct tttcgggcaa tcgtgcaacg cagccagaat
    18121 atgacgaata cgatccatta ttaaacggtg cgccaattac cgaacctgtt gctgtagcag
    18181 ctgctgctac cacggcgaca caaagctggg ctgcgccggt tgaacctgtg actcagacgc
    18241 cgcctgttgc ctctgttgat gttccacctg cacaacctac agtagcctgg cagcctgtac
    18301 cggggccaca aacgggagag ccggttattg ctcctgcaca ggaaggttac ccacagcagc
    18361 cacaatatgc gcagcctgca gtgcaatata atgagccgct gcaacaacct gtacagccac
    18421 agcagccgta ttatgcgcca gcagcggaac aacctgtgca acaaccttac tacgctcctg
    18481 cggcggaaca acctgttcag cagccgtatt acgcaactgc gccggaacag tctgcgcaac
    18541 agtcgtatta cgcccctgcg ccagaacaat cggtggcagg taacgcctgg caagccgaag
    18601 agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc
    18661 cagtcgctca ggagccgttg taccaacagc cgcaacctgt tgaacagcag cctgttgtgg
    18721 agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tattttgaag
    18781 aagtggaaga gaagcgtgcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc
    18841 ctgaaccggt taaagaacca gaaccgatca aatcttcgct gaaaacacct tctgttgcag
    18901 cagtacctcc agtggaagcc gctgccgctg tttcccctct ggcatctggc gtgaaaaaag
    18961 cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata
    19021 gcgctggacc gcgtccacag gtcaaagagg ggattggccc gcagttgcca cgaccgaaac
    19081 gtatccgcgt gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc
    19141 gtgcggcaga agaaaaagcc cgtgaagctc agcgcaatca gtacgattcc ggcgatcagt
    19201 ataacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga
    19261 cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtgaac gcagaagatg
    19321 cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt
    19381 attccggcga acaaccggct ggggcgaatc cgttcacgct ggatgatttt gaattttcgc
    19441 caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca attgttgaac
    19501 ctgtacagca gccgcaacaa ccgattgcac cgcagcaaca gtatcagcag ccgcagcaac
    19561 cagttgcgcc gcagccgcag tatcagcagc cgcaacagcc ggttgcaccg cagcagcaat
    19621 atcagcagcc acaacaaccg gttgcgccgc agcagcaata tcagcagccg caacagccgg
    19681 ttgcacagca gccgcaatat cagcagccgc aacagccagt tgcgccgcag ccgcatgata
    19741 ccctgcttca tccgctgttg atgcgcaatg gtgacagccg tccgttgcat aaaccgacga
    19801 cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
    19861 cctttgcgct tgaacaaatg gcgcgtctgg tggaagcgcg tctggctgat ttccgtatta
    19921 aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa cttaacctgg
    19981 caccgggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt
    20041 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    20101 taccgaataa aaaacgacaa accgtttatc tgcgcgaagt attggataac gccaaattcc
    20161 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    20221 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaagt
    20281 ctgtcggggt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    20341 gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc
    20401 tgttaacgga agtcgttacc gacatgaaag atgctgccaa cgctttgcgc tggtgcgtaa
    20461 atgaaatgga acgtcgttat aagctgatgt ccgccctagg tgtgcgtaat ctggcgggtt
    20521 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    20581 agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaaccg tacattgtgg
    20641 tgttggtgga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctta
    20701 tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaacgcagc
    20761 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    20821 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac
    20881 tgctgggtat gggggatatg ctttactctg ggccgaactc cacgttgccg gtacgtgttc
    20941 atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg
    21001 gtcgcccaca gtatgttgat ggcatcacct ccgacagtga aagcgaaggt ggcgcgggcg
    21061 gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    21121 aaaaacgcaa agcatcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    21181 cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    21241 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcatcgt aggccggata
    21301 aggcgcggta gcgttgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc
    21361 ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccggtcg ggagtgacgt
    21421 aatttgagga ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt
    21481 agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag
    21541 cagcttccac gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga
    21601 aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata tgactcaacc
    21661 tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga
    21721 gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc
    21781 ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt
    21841 cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga
    21901 tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact
    21961 gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg
    22021 cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt
    22081 tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt
    22141 atatcggcca acaacatttg ttggctgcgg ggaagccgtt gccgcgtgct atcgaagccg
    22201 ggcatttgca ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg
    22261 aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtt acttctggcg
    22321 tgaaagagat tcgcgaggcg atcgagcgcg ctcggcaaaa ccgcaatgct ggtcgccgca
    22381 ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc
    22441 cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg
    22501 aacttaactc ggcactgctt tcccgtgccc gtgtctatct gctgaaatcc ctgagtacag
    22561 aggatattga gcaggtgctt actcaggcga tggaagacaa aacccgtggc tatggtggtc
    22621 aggatatagt tctgccagat gaaacgcgac gcgccattgc tgaactggtg aatggcgacg
    22681 cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg
    22741 gtaagcgggt cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct
    22801 ttgataacaa aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg
    22861 gtagtgcacc cgatgcggcg ctgtactggt atgcgcgaat tattaccgct ggtggcgatc
    22921 cgttatatgt tgcgcgtcgc tgtctggcga ttgcgtctga agatgtgggt aatgctgatc
    22981 cacgagcgat gcaggtggca attgcggcct gggattgctt tacccgagtt ggcccggcgg
    23041 aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg
    23101 ctgtctacac tgcgtttaaa gccgcgctgg cggatgctcg cgaacgtccg gattatgacg
    23161 tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg
    23221 aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tatttcccgc
    23281 cggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg
    23341 gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt
    23401 aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat
    23461 ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg
    23521 agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc
    23581 tgggcgctct tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg
    23641 agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc
    23701 ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg
    23761 atgctttaca ggctgaaatt cgcgatatcg cgctgacaat ccctaacctg ccagcagatg
    23821 aagtgccggt aggtaaagat gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc
    23881 gtgagtttga ctttgaagtt cgtgaccatg tgacgctggg tgaaatgcac tctggcctcg
    23941 actttgcagc cgcagttaag ttgaccggtt cccgctttgt ggtaatgaaa gggcagattg
    24001 ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct
    24061 acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc
    24121 tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca
    24181 gtaactatgc gctgatccca acggcagaag ttccgctgac caacctggta cgcggtgaaa
    24241 tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg
    24301 aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag
    24361 ttgaaatggt gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg
    24421 gtcatgcaga aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca
    24481 ctggcgacat gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac
    24541 agaacaccta ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta
    24601 tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg
    24661 gttctggtct ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg
    24721 atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg
    24781 gctaataccc aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt
    24841 tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta
    24901 cctatctctt tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata
    24961 gaaataatta cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc
    25021 attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg
    25081 gtaaaaacga cagcgatcgg cggcctggca atggcaagca gcgcgttaac attacctttt
    25141 agtcggattg cgcacgctgt tgatagtgcc cttccggcaa aatcagacga aaaggttatc
    25201 tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgttgtggac
    25261 ggtgaaatta aatatgttga aacggataat accggcgatg acaactatga cggcctgcac
    25321 caggtgcgcg cctgtctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc
    25381 ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc
    25441 tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc
    25501 aacgagtcta tctatctgaa ctatggtacc ggtacgctgg gcggcaccat gacccgctcc
    25561 tggccgccgg gaaataccct ggtcgctcgc ctgatgaact gctgcggcgg ttatctgaac
    25621 cattacggtg actactcttc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc
    25681 tgggcagatg gtaacagccc gtcggatatc gaaaacagta aactggtagt gctgtttggt
    25741 aataaccctg gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggcg
    25801 cgccagaaat ctaatgctcg catgatcatc atcgatccgc gctataccga caccggtgcc
    25861 gggcgcgaag atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt
    25921 ctggcgtatg tcatgatcac cgaaaacatg gtggatcagg cattcctcga taaatattgc
    25981 gttggctacg acgagaaaac actgcctgcc agtgcgccga aaaatggtca ctataaagct
    26041 tatattctgg gtgaagggcc agatggcgta gctaaaacgc cggaatgggc ctcgcaaatc
    26101 accggtgttc cggcagacaa aatcatcaaa ttggctcgtg aaatcggtag taccaaaccg
    26161 gcgtttatca gccaaggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt
    26221 gctatctcga tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc
    26281 gcgcgtgaag gttcatacag cttaccgttt gtgcgtatgc cgaccctcga aaacccgatc
    26341 cagaccagca tttctatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgaca
    26401 gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg tgccgatcaa aatgatctgg
    26461 aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc
    26521 cttcaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatca
    26581 gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgca
    26641 ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg
    26701 cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt
    26761 gagcaacagt ttactgaagg ccgtacacag gaagagtgga tgcggcatct gtatgcccag
    26821 tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt
    26881 aaaaagcgcg acccacaagg gcaccacgtt gcttataaag ctttccgtga agatcctcag
    26941 gcaaatccac tgaccacgcc atcgggtaaa attgagattt attcgcaggc gctggctgac
    27001 attgccgcga cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctatacg
    27061 ccgggctttg aaagttatca ggatccgctg aacaaacaat atccgctgca gcttactggc
    27121 ttccactaca aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcggct
    27181 tgccgtcagg aaatgtggat caacccgctt gatgcccaaa aacgcggtat caacaacggc
    27241 gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca
    27301 cgaatgatgc cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa
    27361 cgtgtcgata agggaggttg tatcaacgta ctgaccactc aacgtccgtc tcctctcgct
    27421 aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat
    27481 gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga
    27541 gctggcctgt aaagactata aagatctgac gccggaagtc agcttccgcc gcatttatga
    27601 atatgctggt ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta
    27661 ctatctgtcg atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag
    27721 tggtgcgatg cataaacgtg aagatggttt tgttgtagtc gatgaagatg tgtgcattgg
    27781 ctgccgctac tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg
    27841 ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gaggggaaaa agccgatctg
    27901 tgttgaatct tgcccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa
    27961 acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat
    28021 tgtgatcaaa cccaatgcca acagccgccc gaccggggat accactggct atctggcaaa
    28081 cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg
    28141 tcttcgggca atgtgtagcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg
    28201 acctgcgagc agaagcccag cagcgtgtta ttgcctgcat gtttggttta tgggtgctga
    28261 tgggcattgg ctttatcgcc tctatgcttc atcttggttc accgatgcgc gcctttaact
    28321 cgctcaaccg ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt
    28381 ttgctgttgg tggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc
    28441 gtacgctctg gctgatagtg acgatggttc ttggcgtcat ctttgtctgg atgatggtgc
    28501 gtgtgtataa cagcattgat accgttccga cctggtacag catctggacg ccgatgggct
    28561 tcttcctgac gatgtttatg ggcggcccgt tactgggtta cctgttgctg agtctggctg
    28621 gcgtcgatgg ctgggcgatg cgtctgctgc cagccatttc tgtactggcc ctggtagtga
    28681 gtggcgtggt gtcagtgatg cagggcgcag agctggcgac tattcatagc tctgtgcagc
    28741 aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg
    28801 ctgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc
    28861 tactttccgt atcattcatt ctgcttctgg caggggaatt aatcggtcgt ggcgtattct
    28921 atggtttgca tatgaccgtg gggatggccg tcgcaagcta atcataacaa ccggggtttc
    28981 ggccccggtt ctcttttatt tctgcttcgt taacgtgtca taactggtca tcaaattacg
    29041 ataatcagga atatggttag agaacaacgt cgccagtcct tcaatatcat tacgccagtc
    29101 gcgatgcagc tcgcaagcca cgccgaacca tgtcatcaat tgcgcaccag cttgcgacat
    29161 gcgatcccac gccgaatgcc gggtaatttc attaaaggtc cctgacgcgt cagtcacgac
    29221 aaagacgtca aacccttctt caatggctga aagcgccggg aatgccacgc aaacttcggt
    29281 taccacaccg gcaataatta actgtttttt acctgtcgct ttgactgctt ttacaaaatc
    29341 ttcgttatcc caggcgttaa tattgcccgg gcgagcaatg taaggtgcat cggggaattg
    29401 tgctttcagt tctggaacta atgggccgtt aggaccggtt tcaaaactgg tggtaagaat
    29461 agttggtaag ttgaaatact tagccaggtc acccagcgcc agcacgttat ttttaaactt
    29521 atcgggttcg atatcccgta caagggaaag taaaccagcc tggtgatcaa caagcaaaac
    29581 ggcagcatca tttttatcaa gacgaacata cggtttggtc atcctcttct cctttcgaga
    29641 gtcagtggta cgtctgaaat cggtcagacg cgatgcattg ctctgaaagc atagacggga
    29701 aatatgagtt tgctgtgacc atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt
    29761 tagatcaata aaaacaatca gatgagatta atcgtctctc cagcattagc gttatgaatg
    29821 agtaatataa atcgataata cctccgctgc gatatgcgcg tattttcacc cattgacaat
    29881 gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt
    29941 ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga
    30001 tcgcagtgtt aaatacactc gtgccgcttt ggctcgccca ggaacacatg tccacatggc
    30061 aggtaggcgt tgtcagctca tcctatttta ccggcaacct ggtcggtaca ttgctgacag
    30121 ggtatgtcat taagcgcatt ggctttaacc gcagctatta tctggcctcc ttcatttttg
    30181 ccgctggctg tgccggcctt ggcctgatga ttggattctg gagctggttg gcttggcgtt
    30241 ttgtcgccgg cgtcggctgt gccatgattt gggtggttgt tgagagcgcg ctgatgtgca
    30301 gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg
    30361 gaacgttttt aggccagtta ctggtcagca aagtttcaac cgagctgatg tccgtattgc
    30421 cgtgggttac aggtttgacg ttggcaggga tcttaccgct gttgtttacg cgtgtgctga
    30481 atcagcaggc tgaaaaccat gattcgacgt caattacgtc aatgctaaaa ctccgtcagg
    30541 cgcggcttgg cgtgaatggc tgcattatct caggaatcgt tctgggatct ctatatggcc
    30601 tgatgccgct gtacctcaat cacaaagggg tgagcaatgc cagcattggt ttctggatgg
    30661 cggtactggt cagtgcgggt atccttggac aatggccgat tggacgtctg gcggataagt
    30721 ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt cattctcggc agtatcgcga
    30781 tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc
    30841 tatatccggt ggcgatggcc tgggcttgcg agaaagttga acatcatcaa ctggtggcga
    30901 tgaaccaggc cttactgttg agctataccg tgggaagtct gcttggcccg tcatttaccg
    30961 ctatgctaat gcagaatttc tccgataatt tattgtttat catgatcgcc agcgtatcgt
    31021 ttatctattt gctgatgctg cttcgcaacg ccggtcatac gccgaaaccc gttgctcacg
    31081 tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac actctgcttt tttgtttctt
    31141 ctatctgact tgctttattc caaattttat tcgtttaaaa ataaaatgtg cagcaggtta
    31201 taattttgca tttcgctatt tccgcacttc ttatttgccg cgcataatcc ctcgttttac
    31261 agatgcccct ttaattttgg cgaaggattt gtctatggct gggaatgttc aggaaaaaca
    31321 gctgcgatgg tacaacattg cgctgatgtc ttttatcact gtctggggtt ttggcaacgt
    31381 tgttaataac tacgccaacc aggggctggt ggttgttttt tcatgggtgt ttatctttgc
    31441 gctctatttc acaccttatg cgctaattgt tggtcagtta ggctcgacct tcaaagatgg
    31501 gaagggcggg gtcagtacct ggattaaaca cacgatggga cccggactgg cttacctcgc
    31561 cgcgtggacc tactgggtag tgcatattcc ctatctggca caaaaacccc aggcaatact
    31621 gattgcgctc ggttgggcga tgaaaggcga cggttcgtta atcaaagaat attcagtcgt
    31681 agcgttacag gggttaacgc tggtgctgtt tatcttcttt atgtgggttg cttcacgcgg
    31741 tatgaaatcg ctgaaaatcg tcggttctgt ggcagggatt gcgatgtttg ttatgtcgct
    31801 cctgtatgtg gcgatggcgg taaccgcgcc tgcgattact gaagtgcata ttgcgaccac
    31861 aaacattacc tgggaaacgt tcattcctca tatcgacttt acctacatta ccactatttc
    31921 aatgctggtt ttcgcggttg gcggagcaga gaagatttct ccttacgtta atcaaacgcg
    31981 caacccagga aaagaatttc caaaagggat gttatgcctt gcggtgatgg ttgcggtttg
    32041 tgccattctg ggctcactgg cgatggggat gatgtttgac tcgcgtaata tcccggatga
    32101 cttaatgact aacggtcagt attacgcctt tcagaagctg ggcgagtatt acaacatggg
    32161 taatacttta atggtgattt atgccattgc gaataccctg ggacaagtag ctgcgctggt
    32221 attctcgatt gatgcgccgc ttaaagtgtt attaggcgat gctgacagca aatatattcc
    32281 agccagttta tgtcgtacca acgcttctgg tacgcccgtt aatggctatt ttctgaccct
    32341 ggtgctggtg gcgatcctga taatgctgcc gactctcggc attggtgata tgaacaatct
    32401 ctataaatgg ctgttgaacc ttaattcggt agtgatgccg ctgcgttatt tatgggtatt
    32461 tgttgcattt attgcagttg ttcgcctggc gcagaaatat aagccggaat atgtctttat
    32521 tcgtaataag ccgctggcaa tgaccgtcgg aatttggtgt tttgccttta ccgcttttgc
    32581 ctgcctgact ggtattttcc cgaaaatgga agccttcacc gcagagtgga cattccagtt
    32641 ggcgctgaat gttgcaacgc cgtttgtgct ggtgggatta gggctgatat tcccgctgct
    32701 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
    32761 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
    32821 ggtaatacat ttggaagctg tgctgtaagg gcgttagaaa tagagctctg aattatttat
    32881 gcagcgcgga ataaaaaagc ggcaactcaa aaaagttgcc gctttacggg gaaattagaa
    32941 cattacctta tgaccgtact gctcaagaat gcctttcacg cgttccatgg tttctttctt
    33001 cggtggttta acaccatcaa gtttgtattc ttcacccatc gccacccatt tgtgtttgcc
    33061 cagttcgtgg taggggagaa gctcgatttt ctcaacgttg cccatatcac gggtaaattc
    33121 accgaggcga tgcgctgaat cgtcatcgtc agaccagcct ggcacaacaa cgtagcggat
    33181 ccacaccttc acatttttgt tcgccagata tttggcgaac tccagcgtgc ggtggtttga
    33241 aactccaacc agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag
    33301 gtcggttact tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt
    33361 gtccagacag gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac gaacaaactc
    33421 agcttgcagg attgcttcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa
    33481 gtggcgatag gtcaccacct ctttcattaa atcttcaacg gtaacttctt taccgccatg
    33541 cgtgtcccag gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa
    33601 ggtgataaag cgaatacccg ggccgtctac ggttccacag gattcaaagg agtgaatgcg
    33661 accaataact gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc
    33721 gcagctcgaa ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac
    33781 aaaaaaggct ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt
    33841 tactgtacga tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg
    33901 tcctgctgct gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg
    33961 gtcagctgcg gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc
    34021 acgttaacgt tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc
    34081 atcagaccag ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg
    34141 atagagaagg tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa
    34201 gtcagagagg ctactgcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg
    34261 aacggcgcgc cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg
    34321 ttagaagtga tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc
    34381 tgaattttct tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatcg
    34441 ttgttaccaa actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg
    34501 tcacgaatcg gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac
    34561 agaccagcga taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga
    34621 gaggcttcgt agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac
    34681 tgtttagcca gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagttcagg
    34741 agatcgcctt tgatcggctc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg
    34801 ccgccgttga ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc
    34861 atttgtttac caacgatcat cgggcttacg cagcaagcga tagcgtagtc atcgttgttg
    34921 aagtccggac gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact
    34981 ttagcagcga atttcttgaa gttcagcggc agtttttcgg accacagaat ggtcatgttc
    35041 ggttccggag acggacccat ggtgtacagg gtgttcagga aacggaagct gtttttggta
    35101 accagggtac gaccgtcgag gcccatacca ccgatagatt cggttgccca gatcgggtcg
    35161 ccagagaaca gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg
    35221 accaggtggt caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca
    35281 cgttcgatgt acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc
    35341 tgagacttaa cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg
    35401 gtagccggac cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga
    35461 gcgcggtgct gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg
    35521 ttttccagat cagcctgcag agaggtgaac tgtgcaagtt tatctttcat caggtagtcg
    35581 ataccgtaca gcgcaacgcg acggtagtca ccgatgatac gaccacggcc ataagcatct
    35641 ggcagaccgg tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg
    35701 aacacgccct ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc
    35761 agttcgcggt tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata
    35821 agagcacgtt tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttgttg
    35881 atgtagccag cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa gtcaactggc
    35941 gcgtgagtgc ggttttccag tttaacgcct tccattactt tgtcccacag ggtggtggtc
    36001 gcttcagtag cgccagccag gaaggactcg tcaccctcgt acggagtgta gtttttctga
    36061 atgaagtcac ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct
    36121 gtggctaact tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta
    36181 tttactgcgt acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag
    36241 tatgtcaacc caaccaacaa accaccgccg ataatattac cgatcgtaac cggaatcagg
    36301 ttatcagtga tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca
    36361 gcggtccaga attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac
    36421 atgtttgcga tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata
    36481 atgaacgctt tatccatcag gctgcggcca gaataactca tccatactgc cagacatacc
    36541 atcaggtttg ccaggatacc aaggcagacg gcctcaataa aagtatggtg cactttgtgg
    36601 tcggcggttt gtaggacgtt tagtccccat tgaccattcg cggtcatata ctcgccggaa
    36661 agccacatta aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc
    36721 cagtttttcg ccaactgacc ccaggtgatt cgcccactcg ccttagcaac aacaatcaac
    36781 actgtggaag taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag
    36841 caaatgccgc caaccagttt tgccatgccg aagggcattg ttcctgtgcc agtggttgct
    36901 gtgatataga agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa
    36961 gtcttaagcg gatgtttcgt tgctttatag acacctgcct cttcggccac tttggccatt
    37021 gctgcaggaa gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta
    37081 agtcggcgac gagatactaa caaagcatta tag
//
LOCUS       DARNVM010000026        35803 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.026, whole genome shotgun sequence.
ACCESSION   DARNVM010000026 DARNVM010000000
VERSION     DARNVM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.026"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1303
                     /gene="aroP"
                     /locus_tag="SI621_001250"
     CDS             <1..1303
                     /gene="aroP"
                     /locus_tag="SI621_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HEI4063987.1"
                     /translation="LGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGE
                     MVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIP
                     TWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNGGPQA
                     TVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQVI
                     YRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVY
                     NSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESA
                     FGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAV
                     LVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH"
     gene            complement(1346..2200)
                     /gene="ampE"
                     /locus_tag="SI621_001251"
     CDS             complement(1346..2200)
                     /gene="ampE"
                     /locus_tag="SI621_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HEI4063988.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKLETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2197..2748)
                     /gene="ampD"
                     /locus_tag="SI621_001252"
     CDS             complement(2197..2748)
                     /gene="ampD"
                     /locus_tag="SI621_001252"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEI4063989.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVNKETT"
     gene            2836..3729
                     /gene="nadC"
                     /locus_tag="SI621_001253"
     CDS             2836..3729
                     /gene="nadC"
                     /locus_tag="SI621_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEI4063990.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            3933..4373
                     /gene="ppdD"
                     /locus_tag="SI621_001254"
     CDS             3933..4373
                     /gene="ppdD"
                     /locus_tag="SI621_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HEI4063991.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDSGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4383..5768
                     /gene="gspE"
                     /locus_tag="SI621_001255"
     CDS             4383..5768
                     /gene="gspE"
                     /locus_tag="SI621_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEI4063992.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGF
                     YGRTALFEVLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            5758..6960
                     /gene="hofC"
                     /locus_tag="SI621_001256"
     CDS             5758..6960
                     /gene="hofC"
                     /locus_tag="SI621_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HEI4063993.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLIALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTLALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDTMSGMG"
     gene            complement(6995..8038)
                     /gene="guaC"
                     /locus_tag="SI621_001257"
     CDS             complement(6995..8038)
                     /gene="guaC"
                     /locus_tag="SI621_001257"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEI4063994.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8194..8238)
                     /locus_tag="SI621_001258"
     CDS             complement(8194..8238)
                     /locus_tag="SI621_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4063995.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8263..8883
                     /gene="coaE"
                     /locus_tag="SI621_001259"
     CDS             8263..8883
                     /gene="coaE"
                     /locus_tag="SI621_001259"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEI4063996.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            8883..9626
                     /gene="zapD"
                     /locus_tag="SI621_001260"
     CDS             8883..9626
                     /gene="zapD"
                     /locus_tag="SI621_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEI4063997.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQTQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9636..9833
                     /gene="yacG"
                     /locus_tag="SI621_001261"
     CDS             9636..9833
                     /gene="yacG"
                     /locus_tag="SI621_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEI4063998.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(9921..10319)
                     /gene="mutT"
                     /locus_tag="SI621_001262"
     CDS             complement(9921..10319)
                     /gene="mutT"
                     /locus_tag="SI621_001262"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEI4063999.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMSE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10379..13084)
                     /gene="secA"
                     /locus_tag="SI621_001263"
     CDS             complement(10379..13084)
                     /gene="secA"
                     /locus_tag="SI621_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEI4064000.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13146..13658)
                     /gene="secM"
                     /locus_tag="SI621_001264"
     CDS             complement(13146..13658)
                     /gene="secM"
                     /locus_tag="SI621_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HEI4064001.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(13889..14806)
                     /gene="lpxC"
                     /locus_tag="SI621_001265"
     CDS             complement(13889..14806)
                     /gene="lpxC"
                     /locus_tag="SI621_001265"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEI4064002.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(14907..16058)
                     /gene="ftsZ"
                     /locus_tag="SI621_001266"
     CDS             complement(14907..16058)
                     /gene="ftsZ"
                     /locus_tag="SI621_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEI4064003.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16119..17381)
                     /gene="ftsA"
                     /locus_tag="SI621_001267"
     CDS             complement(16119..17381)
                     /gene="ftsA"
                     /locus_tag="SI621_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEI4064004.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17378..18208)
                     /gene="ftsQ"
                     /locus_tag="SI621_001268"
     CDS             complement(17378..18208)
                     /gene="ftsQ"
                     /locus_tag="SI621_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEI4064005.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18210..19130)
                     /gene="ddlB"
                     /locus_tag="SI621_001269"
     CDS             complement(18210..19130)
                     /gene="ddlB"
                     /locus_tag="SI621_001269"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEI4064006.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19123..20598)
                     /gene="murC"
                     /locus_tag="SI621_001270"
     CDS             complement(19123..20598)
                     /gene="murC"
                     /locus_tag="SI621_001270"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEI4064007.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEAQHD"
     gene            complement(20652..21719)
                     /gene="murG"
                     /locus_tag="SI621_001271"
     CDS             complement(20652..21719)
                     /gene="murG"
                     /locus_tag="SI621_001271"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEI4064008.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAVKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQSQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21716..22960)
                     /gene="ftsW"
                     /locus_tag="SI621_001272"
     CDS             complement(21716..22960)
                     /gene="ftsW"
                     /locus_tag="SI621_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEI4064009.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(22960..24276)
                     /gene="murD"
                     /locus_tag="SI621_001273"
     CDS             complement(22960..24276)
                     /gene="murD"
                     /locus_tag="SI621_001273"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEI4064010.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24279..25361)
                     /gene="mraY"
                     /locus_tag="SI621_001274"
     CDS             complement(24279..25361)
                     /gene="mraY"
                     /locus_tag="SI621_001274"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEI4064011.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25355..26713)
                     /gene="murF"
                     /locus_tag="SI621_001275"
     CDS             complement(25355..26713)
                     /gene="murF"
                     /locus_tag="SI621_001275"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEI4064012.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARIKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26710..28197)
                     /gene="murE"
                     /locus_tag="SI621_001276"
     CDS             complement(26710..28197)
                     /gene="murE"
                     /locus_tag="SI621_001276"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEI4064013.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLAELLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCTGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28184..29950)
                     /gene="ftsI"
                     /locus_tag="SI621_001277"
     CDS             complement(28184..29950)
                     /gene="ftsI"
                     /locus_tag="SI621_001277"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HEI4064014.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(29966..30331)
                     /gene="ftsL"
                     /locus_tag="SI621_001278"
     CDS             complement(29966..30331)
                     /gene="ftsL"
                     /locus_tag="SI621_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEI4064015.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30328..31269)
                     /gene="rsmH"
                     /locus_tag="SI621_001279"
     CDS             complement(30328..31269)
                     /gene="rsmH"
                     /locus_tag="SI621_001279"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEI4064016.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31271..31729)
                     /gene="mraZ"
                     /locus_tag="SI621_001280"
     CDS             complement(31271..31729)
                     /gene="mraZ"
                     /locus_tag="SI621_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEI4064017.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32331..33335)
                     /gene="cra"
                     /locus_tag="SI621_001281"
     CDS             complement(32331..33335)
                     /gene="cra"
                     /locus_tag="SI621_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HEI4064018.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33417..33503)
                     /locus_tag="SI621_001282"
     CDS             complement(33417..33503)
                     /locus_tag="SI621_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HEI4064019.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33515..34006)
                     /gene="ilvN"
                     /locus_tag="SI621_001283"
     CDS             complement(33515..34006)
                     /gene="ilvN"
                     /locus_tag="SI621_001283"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEI4064020.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34009..35733)
                     /gene="ilvI"
                     /locus_tag="SI621_001284"
     CDS             complement(34009..35733)
                     /gene="ilvI"
                     /locus_tag="SI621_001284"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEI4064021.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQIAHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8522 a        10058 c         8806 g         8417 t
ORIGIN      
        1 gctgggtggc gcgataggga ccgggctatt cctgggtagc gcctccgtaa tacagtccgc
       61 agggccaggg attatcctgg gttacgccat tgctggtttt atcgcctttc tgatcatgcg
      121 tcagctaggt gaaatggtgg tcgaagaacc tgtcgcaggc tcctttagcc actttgctta
      181 taaatactgg ggcagttttg ccggtttcgc ctctggctgg aactactggg tactgtacgt
      241 tttagttgcc atggcagagc ttactgctgt gggtaaatac attcagttct ggtatccaga
      301 aatcccaacc tgggtttctg ccgccgtatt ctttgtggtg atcaacgcca tcaacctgac
      361 caacgtaaaa gtgtttggtg agatggagtt ctggtttgcc attatcaaag ttatcgcagt
      421 ggtagcgatg atcatcttcg gcggctggct gctattcagt ggcaacggcg gcccgcaggc
      481 gaccgttagc aacctgtggg atcaaggtgg tttcctgccg cacggcttca ccgggctggt
      541 gatgatgatg gcgattatca tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc
      601 agcagaagct gataacccgg agcaaagtat cccgaaagca actaaccagg ttatctaccg
      661 catcctgatt ttctatattg gttcgttagc cgttctgctc tcgctgatgc cgtggacccg
      721 cgttactgct gataccagtc cgtttgtact gatcttccac gaattgggcg atacctttgt
      781 ggcgaatgcg ctgaacatcg tggtactgac tgctgcgctc tccgtgtaca acagctgcgt
      841 atattgcaac agccgtatgc tgtttggtct ggcacagcag ggcaacgcgc caaaagcgct
      901 ggcgtctgtc gacaaacgcg gtgtaccggt taacactatt ctggtgtctg cgctggttac
      961 ggcattgtgc gtgctgatta actaccttgc accggaatcc gcattcggac tgttaatggc
     1021 gctggtggta tctgcactgg taatcaactg ggcgatgatt agcctggcgc atatgaaatt
     1081 ccgtcgcgcc aagcaggaac aaggcgtggt aactcgcttc cctgctctgc tttatccgct
     1141 gggtaactgg atctgcctgc tgtttatggc ggcggtactg gtgattatgc tgatgacccc
     1201 aggaatggcg atttcggtat acctgatccc ggtatggctg atcgtgttag gtatcggcta
     1261 tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat taatctctct acgccctcac
     1321 ccatacaggg tgagggcaat aatctttaca ccaacgcccc gtaaatcgtc agtagcgcaa
     1381 tcaccaccac gaccacgaac gaggttttct tcgccatgga aaccgctgcc ttcggcgtct
     1441 ccagcttatc gacatgcggt tcacgcgcca gagagaactg cgccagacgc gttaatacct
     1501 gatattgcga agtatggaaa tcacccagcg aagcaaacca ggccggtaac gctttctccc
     1561 catgaccgat caaggcatat accacacccg caagacgaac cggcacccaa tccagtacat
     1621 gaagcacggc atcaatgccg gactgtaaac gatgatgcgg cgtctggtaa cgcgccagcc
     1681 agtattgcca tgcacgcaaa aacgcatacc ccatcagcgt aacgggtccc caggttcccc
     1741 ccacaatcag ccagaacagc ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat
     1801 tttgcagctc acgcaaatac tcacgttcat cgcagcctgc cgggacgccg tgaatcatgg
     1861 tgagttcgcc agccatcgtg gcacgggcat ggctatcatt acgtgaagca gctgtcagat
     1921 aagcatgata atgaagacga actttacctg cgccaataca cagcaaacca atcagcagcc
     1981 acaccagcag cgtgggaacg ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag
     2041 tcacgcccat cgcaataatg gtcatgccta atgtgcgccc gagagaaaaa tgtttcaccc
     2101 gccgaaagaa cgcttcaaga cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc
     2161 gctcgaaaat taacaccagt aaggttgtaa atagcgtcat gttgtctcct tgttgaccag
     2221 cgcacgaaac cgtgcccaat caaatgcagg accgggatcg gttttccgat ccggcgcaat
     2281 atcacaatgg cccgtcatgt tattagcgat atctggatag cgatcaatca gtgcccgcgt
     2341 aaccgccgca agctgttgat actgcgcatc ggtatacgcc agcgtatcgg tgccttccag
     2401 ctcaatccca atagaaaaat cattgcagcg ttcgcgcccc tgatactgag agactccggc
     2461 atgccaggca cgtttatcga aaggaacata ctggactatt tcaccatcgc ggcgaatcaa
     2521 acagtgagcg gagacgcgca aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc
     2581 aatagttcca gtgaataatg cgtcgatcca cggaccgcca aactcgcctg gcggcaggct
     2641 aatattgtgc accaccagca gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg
     2701 tgagggaacg cggcgcgcgc caaccagcca cccctgttct aacaacatgc aggatctcct
     2761 tatatgtggt gctaataccc ggttcagagt agcatgtttc taccttatga ttcgttagct
     2821 atctggagtt ttaacatgcc gcctcgccgc tataaccctg acacccgacg tgacgagctg
     2881 ctggaacgca ttaatctcga tatccccggc gcggtggccc aggcgctgcg ggaagattta
     2941 ggcggaacag tcgatgccaa caatgacatt acggcaaaac ttttaccgga aaactctcgc
     3001 tctcatgcca cagtgatcac ccgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa
     3061 gaggtgttta ttcaactggc aggcgacgat gtcaccataa tctggcatgt ggatgacggc
     3121 gatgtcatca acgccaatca acccttgttc gaacttgaag gcccatcccg cgtgctgtta
     3181 acgggcgaac gcactgcgct taattttgtg caaacccttt caggggttgc cagtaaggta
     3241 cgccactatg tcgaattact ggagggcacc aacacgcagt tgttggatac gcgcaaaacc
     3301 ttacccggcc tgcgttcagc tctgaaatac gcggtacttt gcggcggcgg agcgaatcac
     3361 cgtctggggc tttctgatgc ctttctgatc aaagaaaacc atattattgc ctccggctca
     3421 gtgcgccagg cggtcgaaaa agcatcctgg ctgcacccgg atgcgccagt agaagtcgaa
     3481 gtagagaatc tggaagaact tgatgaagcc ctgaaagcag gtgccgatat catcatgctg
     3541 gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta
     3601 ctggaagtgt ctggcaacgt cactgacaaa acactgcgcg aatttgccga aacgggcgtg
     3661 gactttatct ccgttggtgc actaactaaa cacgtacagg cactcgacct ttcaatgcgt
     3721 tttcgctaat tgctaacaga tttatccgct ccatcgcggg cggataaatc tccccttccc
     3781 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
     3841 tcggaagctg ctttcccgtt tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa
     3901 agtagcgcca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
     3961 tcgaactgat ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt
     4021 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
     4081 gtaccgccgt agagttgtgc gcgctggaac atggtggatt aaatacctgc gacagtggca
     4141 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
     4201 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacaccgg
     4261 gttgggataa cgcaaacggc gtcaccggtt ggacgcgcaa ctgcaatatt caaagtgaca
     4321 gcgcattgca gcaagcctgc gaagatgtct tccgttttga tgacgccaac taaggagcgg
     4381 caatgaatat tccacagctc accgccctgt gtctgcgtta tcagggagtc ttgctggatg
     4441 ccagcgaaga ggtggttcat gttgcggtgg tcgatgcccc ttcgcatgag ctactggacg
     4501 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
     4561 aaggtcacgc cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc
     4621 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
     4681 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
     4741 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc
     4801 tgggcaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
     4861 tggcaggaaa cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
     4921 tggtattaag attgttacag caggtgagtc aggcactgga tgtcaacacg cttggaatgc
     4981 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
     5041 taacaggccc tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaacgctga
     5101 atactgctga tattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac
     5161 taaaccagac gcaaatccat ccgcgtgccg gtcttacctt tcagggcgtt ttgcgtgcgt
     5221 tattgcgcca ggaccctgac gtcattatga tcggagagat ccgcgatggc gaaacagcag
     5281 agatcgctat taaagcggcg caaaccggtc acctggtgtt gtctacccta cacaccaact
     5341 ccacctgcga aacgctggtg cgtttacagc aaatgggcgt tgctcgctgg atgctctcat
     5401 cagcgcttac gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc
     5461 gacagcaagg ggagccaatc catattccag acaatgtatg gccgtcgccg ctgccccgct
     5521 ggcaagcccc tggttgtaga cactgctacc acggttttta tggtcgtacg gccttatttg
     5581 aagttctacc cataacaccg atcatacgcc agcttatttc cgctaatacc gacgttgaat
     5641 cgctggaaac acacgcccga caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg
     5701 ccgtggagca aggcttaacc acctttgaag agttaatccg cgtattgggg atgccgcatg
     5761 gcgagtaagc aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg
     5821 atgctatggg ctgagagccg tgctttgctg ctcatagcac tacagcaaca gatggttacc
     5881 ccacttagcc tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg
     5941 gaagtcattc atcaactggc gacgctactc aaagccgggt taacgctttc tgaagggctg
     6001 gcactgctgg cggaacagca tcccagtaag caatggcaag cgttgctgca atcgctggcg
     6061 cacgatcttg aacagggcgt tgccttttcc aatgccttat taccctggtc agaggtattt
     6121 ccgccgctct atcaggcgat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc
     6181 tgctttgaac tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca
     6241 gcgttacgtt atcccattat cattttagcg atggcaatca tggtggttgt ggcaatgctg
     6301 cattttgttc taccggagtt tgccgctatc tataagacct ttaacactcc gctaccggcg
     6361 ctaacgcagg ggatcatgac gctggcaagc tttagtggcg aatggggttg gttgctggtg
     6421 ctattcggct ttctgctggc gatagccaat aagctgctga tgcgccaccc aaccaggctt
     6481 atcgcacggc agaaattgct gttacgcatc ccgattatgg gttcactgat gcggggacaa
     6541 aaactcacac agatctttac gattctggcg ctgacacaaa gtgcaggcat tactttttta
     6601 cagggcgtag agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca
     6661 caaatccagc acgatatcag taacggtcaa cccatctggc tggcgctaaa aaatgccggt
     6721 gagtttagcc cactctgttt gcaattagtg agaacaggag aggcatccgg ctcgctggat
     6781 ctcatgttag acaacctcgc ccatcatcat cgggataaca cactggcgct ggcggataac
     6841 ctcgcagcct tactggaacc ggcgttgctg atcataacgg gaggaattat cggtacgctg
     6901 gtggtggcaa tgtatctgcc cattttccat ttaggcgata caatgagtgg aatgggataa
     6961 cgctggcgtg ttgctccacg ccagcgttgg gagattacag gttgttgaag atgcggtttt
     7021 cttgttccag cacacggata aatgtggtgc gcttggtcag ctctttcagg cgtgaagccc
     7081 caacgtatgt acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg
     7141 ggcctcgcag cggcagctta acggttttac cttctgctgc gcgatattcc gcaacgccgc
     7201 caacgtgacg tttcatcgca gactcggagc tcatgccgta gaacagcata aatttctcgc
     7261 cgttctcctc aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca
     7321 tgacgaaatc ggcaccgccg ccaaaggctt tcgcgacatc gcccggcgtg gtgcagccac
     7381 cgtcgctgac gatcattccg cccagaccat gcgcagcatc ggcacattcg atcaccgcag
     7441 aaagttgcgg ataaccgacg cctgttttga cgcgagttgt acaaacagaa cctgggccaa
     7501 tgccaacttt aacgatatca gcaccggaga ggataagctc ctcacacatt tcaccagtca
     7561 ctacgttgcc agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact
     7621 gcacgaagtg ttcggaataa ccattcgcca cgtcaataca aacgaaattt aatgccgggt
     7681 tcaggtcgag aatctgttta gttttttcga aatccgcatc tgatgtaccg gtagaaacca
     7741 tcacatgttt cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag
     7801 aatagtgttt atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa
     7861 atgtgcctac ggtgtccata tttgcggcga taatcggcac gccggaccag ctctgacctg
     7921 aatgtttgaa ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc
     7981 gtttagggcg gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt
     8041 gcggattcct ggggttaatg gctataaggg taagagcgca actccagtga cgttatcata
     8101 cgcactaatc aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata
     8161 aatttacctg gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cgctcatttt
     8221 taacgttttg ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc
     8281 ttaacgggag gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga
     8341 attaacgtca ttgatgccga tattattgcg cgtcaggtgg ttgaaccagg cgcacctgcg
     8401 ctacatgcca ttgctgatca ctttggcgct aacatgattg ctgctgatgg aacattgcag
     8461 cgccgggcct tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgcc
     8521 ctgctgcatc cgctgattca gcaagagacg caacaccaga tccagcaagc tacttccccc
     8581 tatgtactat gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga
     8641 gtgctggtgg tggatgtcag cccagaaacg caacttaagc gcaccatgca gcgcgatgat
     8701 gtaactcgcg agcatgttga acaaatcctt gctgctcagg caacgcgcga agcccgcctt
     8761 gccgtggcag atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt
     8821 gcccgcctgc acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg
     8881 taatgcagac ccaggtcctt tttgaacatc cactaaatga aaaaatgcgt acatggctgc
     8941 gcattgagtt tttgattcag caactcaccg ttaatctacc catcgttgac cacgctggcg
     9001 cgctgcattt cttccgtaat gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc
     9061 gcactgagct gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg
     9121 tgcctggcgt ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga
     9181 gcgtattaat ttccgcgccg cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc
     9241 tggtgcgtca gcgactgagc atcccaggcg gctgttgcag ctttgattta cctacattgc
     9301 acatttggct gcatctaccc cagacgcagc gcgacagcca ggtagaaacc tggattgcca
     9361 gcctgaaccc gctcacccag gcacttacca tggtgctgga tttaattcgc cagtcggccc
     9421 ccttccgtaa acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact
     9481 tgctgcgtct gaatctgtcg ctcgattcac agctttatcc gcaaatttcc ggtcataaga
     9541 gccgttttgc cattcgtttt atgccgctgg acagtgaaaa cggacaggta ccggaacgtc
     9601 tggatttcga actggcctgt tgctaaggag taaaaatgtc agaaactatt acggtgaatt
     9661 gcccaacctg cgggaaaacg gtggtgtggg gcgaaatcag cccgtttcgg ccattttgtt
     9721 ccaaacgttg ccagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca
     9781 gcagtggcga tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacattttg
     9841 tctgatgcga cgctggcctg ataagacgcg ccagcgtcgc atcgggcatc ttgtgcaaat
     9901 gttggatgcg gcataaacgc ctatccgaca tatacacgct taagcttcgc aagtaccggt
     9961 tcattggctg gcggaaaatc atcggcattc agaccgacca gcgacatcca ctcaccgggt
    10021 tgcccttctt taccccacgg ctccccttcc cagctttcga ccagccagaa ccacagagtt
    10081 atatgcctgt ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgcggggta
    10141 atcccgactt cctcctgaag ttcacgcacc accgcctgtt ccggcgtttc gctcatttca
    10201 attttaccgc cgggaaactc tagtttattc gccatgtgcg catctgctgc gcgacgggtt
    10261 ataaagattt cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt
    10321 gtcttaaacc tataaaaaag gcgcagaatc ctgcgccttt tacttcaact gttagctttt
    10381 attgcaggcg accatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt
    10441 tacgtcctac tttgcgctca ccggtttgcg ccgccagtgc agctgcagct gcagagtcgt
    10501 catcctgatg gctaagctgc tgcatctgcg ctaaacgctc ggcttccata cgacgctgtt
    10561 gttccagctc ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa
    10621 cttcatattt caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt
    10681 cctgcttcgg atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca
    10741 tcgccgccag gtgctctttc cacagagagt caagcgtttg cagcatgacg cctttttcga
    10801 agtgacgcat catctctgca ccaaccactt cttctttacg ctgatacact tcgatggact
    10861 gcgccagaat gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact
    10921 cggcaattgg caaatcaagg tcgaagtcgt tcttcagacg ttcctgcagc cccggaatat
    10981 cccacatttc ttccagcgac tgtggcggaa tgtaggcatc aatggtcgct ttgaacacat
    11041 cttcacgaat gctgttgatg gtttcactca catcgctgac atccagcagt tcgttacgct
    11101 gggagtaaat ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac
    11161 gaatgtcgaa gttacgactt tcaactttac gctgggcatt ggcaatcgct ttagtcaccc
    11221 acgggtgttc aatggcttcg cctggcttca tacccagttt acgcatcatg ccggatactc
    11281 ggtcggaagc aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac
    11341 cagcatcccc ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt
    11401 gacgctcggt accgatgata tgcaggccac ctgctgccag taccgcatcg tgacgtacct
    11461 gccagtcggc tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt
    11521 ctgcctgcca gctaccaccg agcacaatat ctgtaccacg acccgccatg ttggtcgcga
    11581 tagtcaccgc agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt
    11641 tggcgttcag gacgttgtgc ttaataccgg ctttggtcag ttcgtttgat accagctccg
    11701 atttttcgat ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga
    11761 tatcttcaat gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat
    11821 ctttacgaat cattggacgg ttggtcggaa caacgacggt atccagcttg tagatggagc
    11881 tgaattcgaa agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac
    11941 ggaagtagtt ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac
    12001 cttctttcgc ttccacagcc tggtgcagac catcggacca gcgacgcccc tgcatggtac
    12061 gaccggtgtg ttcgtcaacg atgataactt caccatcttt aacgatgtaa tcgacgtcgc
    12121 gggtaaacag cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgtttg
    12181 ccggagagta cagagactcc ccttcatcca tgatgccttc tttcaccagc agttcttcaa
    12241 tcaggaccag accacgttcg gtcaggttca cctgacgaga tttttcgtcc accgagaagt
    12301 ggccttcgcc ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa
    12361 ttttattcac gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg
    12421 gtgtacgagc ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt
    12481 tacgctgtac acgttcttca gggctgaacg ccatgttatc gcgcaggtag tcaaagccgt
    12541 attcgttgtt cgtaccgtaa gtgatgtcag ctgcgtaagc ttcacgcttc gccggtgctg
    12601 gcatgcccgg caggttgata ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt
    12661 tttcggcgtc acgttgcgcc aggtagtcgt tgacggtaac aacgtgtacg cccttaccgg
    12721 ttaatgcgtt caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca
    12781 tttcggcgat gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac
    12841 gcataccaaa gacgcgctta cttgcctcac gcaccacggc gaaagcttcc gggatcagat
    12901 tttccagcac ttcgcctttt tccagacgtg cacgaaactc tgcggttttc cctttcagtt
    12961 cttcgtcgga gagtttttcc atctccggtt ccatggcatt gatgatgtta accactttgc
    13021 gcatccggcg cagggtgcga tcgttacgac taccgaaaac tttagttaat aatttgatta
    13081 gcataataaa atctcaaacg ccccgcgttg cggagtcaat aaaatgaagt aaaggtttat
    13141 tgttgttagc tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg
    13201 ggcgtgctga attttgcttg cggggtaaaa tgcgcataat caatgcgata acccttttca
    13261 gacggcgtgc cttcctgggt caacagcgcg ctgagcgtat ccagtaatgc aagatgttgc
    13321 gcctgaagag gcaaagattc ttcagcaacg ggcagcgttt gcggtgccat tgcgaaggaa
    13381 agatgacgga ttaccgtgcg aatggcatgt tgatgccagt aatcaacgga atagttcgaa
    13441 ttcgggcggc gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa
    13501 ggctcgtggt tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc
    13561 gcaggcaaac ctaaactcgc cgcgaccatc cctaataaga gatgcggcca gaagtagcgt
    13621 ttaccaaact gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgtat
    13681 tcaaacgtat ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa
    13741 gataagttgt gcgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatcgttctt
    13801 ataaaattca aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt
    13861 cgaattttat acgacagtat aaatgtcgtt atgccagtac ggctgaaggc gctttgaagg
    13921 ccaacggcag ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgttttgcaa
    13981 ggacagcctg cagcagtttg ttattcagtg catgaccaga tttataagcg gtaaatgcac
    14041 caataatatt gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtgacgca
    14101 caaattcgtc ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga
    14161 tggcacaatc gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac
    14221 gcatgaaacc gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt
    14281 tcatcgcata gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat
    14341 ccagcgaaaa accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa
    14401 cagtctcttt gatgcgaaca aatttcttgg cgcagttcag ctcgtcgata ccggcgtcaa
    14461 gcagcaggta tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa
    14521 cttcgataac aatgttatcg atgcccaagc ccgcgagagc agcattgagg tgctctacgg
    14581 ttgaaatccg tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag
    14641 atttggcatc ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc
    14701 cggtgttggc cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga
    14761 cacccgtcgc ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc
    14821 aaattaccta tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc
    14881 ctcgaaaccc aaattccagt caattcttaa tcagcttgct tacgcaggaa tgctgggata
    14941 tccagataat ctggctcttt cgcagtttgc ggcgcattgt cattcacgac tttagcaacc
    15001 ggcttctgct cctgggtcag cggagccatc ccgtgctgct ggtagcgatc catcactggc
    15061 tgctgaacct gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct
    15121 gtcgcaacaa cagttacgcg cagctcgtca ttcatatccg ggtcaagaga agtaccgata
    15181 accacagtcg cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca
    15241 tccagacgca ggtcgaagcc cgccgtgatg ttaaccagca cgccgcgtgc gccagacagg
    15301 tcgatatctt caagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct
    15361 tcaccgctcg ccacgccaga acccatcatt gcgtagccca tttcagacat tacggtgcgt
    15421 acgtctgcaa agtccacgtt catcaaaccc ggacgagtaa tcagttcggc gataccttgc
    15481 acagcgcctt tgagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg
    15541 cccagaactt tcagcagttt gtcgttcggg atagtgatca gagagtccac atgtttggac
    15601 agttcagtga tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa gttgaaaggc
    15661 ttagtgacga cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactgga
    15721 gctgcgcctg taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct
    15781 tccagcgccg cacgcagtgc atcgcgatcc tcatcagccg cattacggcc aacttctgga
    15841 ttagcgccag cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc
    15901 gctgttttac gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaatg
    15961 cgctcgcgca ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta
    16021 atcaccgcgt cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct
    16081 gtcgcctgag gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa
    16141 ctattgagtc gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct
    16201 tcaccgttaa gatgtgactc ttttccatag tgaagcaatc ccaccgccgt cgaataatac
    16261 ggctcctgag cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc
    16321 gtatgaaaca cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccagtt
    16381 aatacaatgc ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac
    16441 tgcaatatct cttcgttgac caggttgagc agctcggtat agcgcggctc gatcacctct
    16501 gccagggtct gacgttgcag actccgcggc ggacgaccgc ctacgctcgg cacttccacg
    16561 ctctcatctt ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg
    16621 gcgtcgcttg gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca
    16681 taaggaatta ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat atccattgta
    16741 ccaccaccga tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa
    16801 taacttgatg ccagtccggc aaatatcagt tggtcaactt tcagcccaca acgttcaacc
    16861 gctttgacga tgtttttcgc catatcgttg tgacatgtga tcaggtgcac ttttgcctgc
    16921 atccgcacgc ccgaaagtcc taccggattc ttgatccctt cctgatagtc aatcgcatac
    16981 tcttgcggga tcacatgcag cacacgatgc tcatcgcgca cacgcaccga tttcgcggta
    17041 tggacgacgt tttccacatc ttcttgcgtc acttcttctt cagaaatagg caccatacca
    17101 atttcattct ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga
    17161 caatctgcca tcaattctgc ctggtcaatg gcacgttgta cgcacttgac cacggattcg
    17221 aggtcgttca ccccgccttt atccatacca cgtgacgggc agctgcccac gccaatgata
    17281 ttgaccatac cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatc
    17341 tccagtccta ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct
    17401 gattttgttg ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag
    17461 agtcataacg caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta
    17521 aaaccggata aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat
    17581 tgagcttaat atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt
    17641 ccttcagagt aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca
    17701 cttcattagc gctgccttcc gggccataca gcattggaag cacctgcttg ctggtgcgat
    17761 ctggcggcac gctgaaggtg tttccttccg cgtctaccat gtgttgatca ttccaccgcg
    17821 caatcggcac atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc
    17881 tcacctgctt aatccacggc aggcgttgtt ctatttgcgt ctggatgatg ttgacatcct
    17941 gggtcataaa ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac
    18001 gcgtgtaatg gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt
    18061 ccatccagcc caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga
    18121 aaaggatccc cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt
    18181 ttcgcgtgtt cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc
    18241 aactgcgaga agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc
    18301 atacccggtg aggtattggc ttccagcaga taaaactgtc catcgctgtc cagcataacg
    18361 tcaatacgtc cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc
    18421 aaattggcct cttgtgacgc ttccagacct gcggggcaga aatactgtgt ctcatcagag
    18481 agatacttcg cctcataatc atagaaggtt ccggacggtt gaatacgtat tgacggtaaa
    18541 atttcttcac cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat
    18601 acttcttcat cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact
    18661 acttttgaca ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaacct
    18721 aaggcagaaa tttctgctaa ctgcttatca ctcaggcctt tttcaaactc tgcgcgggtt
    18781 aacgctaccc acggagcgac cggtaaaccg gcaccttgcc acagaagttt gctgcgtagt
    18841 ttatccattg aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc
    18901 tcgagcatgc cctgcagcgt gccatcttca ccgccgcgac cgtgtagcgc gataaacact
    18961 ttctgaaagc ccatcgactt cagttgcgtc acgtcgactt ctttcgggtc gacaggatac
    19021 gcgtcaatac cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact
    19081 tcccgctcag cggaggtccc acccaacagg accgcgattt tatcagtcat gttgtgcttc
    19141 ctccggagtt tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccaatatt
    19201 accagccccc tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc
    19261 tacctgcgcc gggtcaggca ccagaatggg atcaattttc ccacgtccac gaattgtgcg
    19321 acacagcgaa cggctgtccg ctcccggaat tggcgcttcg ccagccggat acacttccag
    19381 catcaacagg gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgtgt
    19441 acgggtaaaa cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcacg
    19501 cgccgcttta atagtggcgt ccacttccgt cgggtggtgg ccgtagtcat cgaccaacat
    19561 tgccgtaccg cttttaccat tcactggctc cagcgggaat tcaccgagga aatcaaaacg
    19621 acgaccagta ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt caattccctc
    19681 ttccgtagca accgcaaccg cagctgcggc gttcagcgcg ttatgacggc ctggcgcatt
    19741 cagggtgacg cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc
    19801 aatctgctga taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagtcgtggt
    19861 ctgacgtccc acacgcggta acaattcgcg gatcaccgga tcatcaacac acatcactgc
    19921 acgaccgtaa aacggcaggt tgtgcagaaa attaataaag gtctgtttta aattctcaaa
    19981 gtcgccctgg taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg
    20041 ttgcagatgc aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg
    20101 ccccaaacgc gcatgaacgc ccgccgcttt taccagccca ccgttaacga aggttgggtc
    20161 gagtcccgct tctgcgtaga tgctggaaac catcgcagtg gttgtcgttt tgccgtgcgt
    20221 tccggcaatg gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg
    20281 gatcaccgga atacgcgctt catgagctgc gacaatttcc gggttatcgg cagaaatcgc
    20341 gctggaaaca acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat
    20401 cgtcgcccca agattcatta actgctgcgt gaccggattt ggcgctaaat cggaaccact
    20461 gatctgataa ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccgat
    20521 gccgacaaaa tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc
    20581 caattgttgt gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg
    20641 catcgctaca attacgcccg ggcagcccgg ctcacttcat ttgccactcg ctcggtggca
    20701 tccggaatgg atgcagcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac
    20761 ccggccaggg tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct
    20821 gcgcccgctt tttccagcgg tagagcattc cagtattgct ggcggtcttt atgttgaaac
    20881 ggcacaaaca acgccggtag tcctgccgca gcgatttcac tcaccgttaa cgcaccggag
    20941 cggcaaacga cgacatccgc ccacgcatac gccgccgcca tatcatcaat aaattccgtc
    21001 actttatgct gcgattgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct
    21061 ttgccgctct gatgccagat agtgactgaa tcacccagtt tcacagcaac ctgcggcatt
    21121 gtctggttaa gaatgcgtgc gccctgggaa ccacccacta ccaacacacg aaccggacct
    21181 tcacgtccag ccaaacgttg ctgcggcaac ggcagcgcca gcacatcggt acgcaccggg
    21241 ttacccacca cttccgcatt agggaaagcg cctggaaacg cctgcatcac tttggtggca
    21301 atcttcgcca gccatttatt ggttaagccc gcaataccgt tttgttcatg aagtacaacc
    21361 ggaatgccta acgaccacgc ggccagacca cctgggcctg atacgtagcc gcccattccg
    21421 agcaccacgt caggtttgta cgctttcata atcgcccgcg cctgacgcca ggcgttgaag
    21481 attcgcagtg gcgcagctat cagtgctttt ataccttttc cacgcagacc agagatacga
    21541 atgaaatcaa tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc
    21601 cccagccagc gaacttgcca accctgagcc attagatggt gcgcaaccgc cagtcccggg
    21661 aatacatgtc caccggttcc gcctgccatc accattaatc gctttccttg accactcatc
    21721 gtgaacctcg tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca
    21781 gcatcatgat ggctgtcgac ataatcagta agctcgaacc accgtaactg atcagtggca
    21841 atgtcagacc tttggtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga
    21901 agctaaacca gatgccaatg gaacaggcga gaaaaccgga aaaacgatgg tcaatttcta
    21961 atgctttacg gccaatcgac atcgcacgaa aagcgacgaa gaataccatt aaaagtgcta
    22021 gtaccacacc gacataacct aattcttcgc cgataatggc gaaaataaag tcagtgtgcg
    22081 cttccggcag atactccagt ttttgtaccg agttacctaa gccttgcccc caaagttcgc
    22141 cgcgaccaaa cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc
    22201 acgggttcca gaatgcggta acacggcgga tacggtacgg ttcggcgagg atcagcaaca
    22261 caaccgctga aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca
    22321 ggaataacat cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg
    22381 ccagcagtaa cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt
    22441 tattacgtac ttcgtcgcct ttacgcacca gatagttggc gatatagcaa aacaacgaca
    22501 gttttgtcag ctccgcaggc tggatacgca gcaagccgag atcgatccaa cgcgatgccc
    22561 ctttaacaga gctacccact accagaacga tcatcaacag gatgattgat ccgagtagca
    22621 tcgtagcgct gtaacgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa
    22681 tgaatgccag gatcagatag acgccatcac gcttcgcgaa gaagaacgga tcgttggtta
    22741 agcgttgccc gatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcgaggc
    22801 cgaaggtcag ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc
    22861 gcgagcccat cacccagccc tttagcgccg tggagatcca gaccagaata ctgaatcctg
    22921 gcaggcgcgg cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag
    22981 acgggcaaac tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc
    23041 cggggagagc agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatcgcctg
    23101 ttccatggtt tcggtttgtt ctgccacttc cgggcgtagc gccgccagct gcgcgccgtc
    23161 ccgaccgaaa caatacagac gtacgttatc gccattcagg taacgcgcca gtgggctaaa
    23221 atctgccgat ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cgtgcaggcc
    23281 attcagtgcc gcttccgtac tgccgacgtt ggtcgctttt gaatcgttaa tccagcgcac
    23341 accgttatgc tccagcacaa cttcaaagcg atgcggcaga ccagtgaatg tggttaacgc
    23401 tttcaggctg ctggcgcgcg gcaaaccagc ggcatctgcc agtgccagag ctgccagtgc
    23461 attggtgtag ttatgctgcc cggaaagttt catttctttc acgttcagca ctttctcgcc
    23521 cttcacccgc agccaggttt caccctgctg atgattcagg tgatagtcac ccatgttgac
    23581 gccaaagctg acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc
    23641 attaaccacg caaactttcg cgttttcgta aatgcgcagt tttgctgcac gatactgttg
    23701 taaaccaaac ggatagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac
    23761 cgcctgtaaa ctggaggtgg tttcaagctg gaagctcgac agctccagca cgtacagttc
    23821 gcactcatca tccagcagca tcaacgcagg caggccaata ttgccaccca caccaacgtt
    23881 aacccccgcc gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga
    23941 accggtgatc gccacaatcg gtgcttgtgc ttcacgacag aacagctcga tatcgccaac
    24001 gatttcgatt ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg
    24061 actggcgaca atcagatcgg ccgccatcag ccattcatca ttcagaccgc ccgtgtggcg
    24121 ttctacagct tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg
    24181 cggcgtcaca ccgcgagcga ggaaaaagtc cacgcaagaa agcccggtga ggcccaggcc
    24241 gataatgacg acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc
    24301 agaccaatca ggaccagcat cagcgaaata atccagaaac gtacaatgac gcgcggttcc
    24361 ggccagcctt tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg
    24421 cgcagtttaa aggagccgac ctgcaggatg acggaaagcg tttctaccac gaacacgccc
    24481 cccataatca ccagcaggaa ttcctgacgc agcagtacgg cgataatacc taacgcacca
    24541 cctaacgcca gcgaaccgac atcgcccata aagacctgcg ccggataggt gttaaaccac
    24601 aggaagccca gccctgcccc gactatcgcg gtacagacaa tgaccagttc cccggcgtgt
    24661 cgtagatacg gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc
    24721 agcgcaaaac caccggcgac aaatacggtc ggcataattg ccagaccgtc gagaccatca
    24781 gtcaggttta ccgcgttgcc tgtacccaca atgacgaagt aagccagcag aatgtagaac
    24841 agccccagtt gcggcatcac atctttaaag aacggaacca ccagctgcgt tgcgggcgtg
    24901 tctttgccgg caaggtacag cgcgaaggca acacccagcg caatgaccga catccagaaa
    24961 tacttccaac gagcgatcaa ccctttggtg tctttacgca ccactttgcg atagtcatca
    25021 acaaagccaa tgatgccgta acctaccagc accaccagta cgcaccagac gtacggattg
    25081 gatggataag cccacagcag cacggagatc acaatcgctg tcaggatcat aatcccgccc
    25141 atggtcggcg taccgcgctt gctgaagtgt gattcaggac cgtcgttacg cactacctga
    25201 ccaaaggaaa gtttttgcaa atgagcaatc atacgcgggc ccatccacaa tgagatgaac
    25261 agcgcggtca gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg
    25321 gaataatatt tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag
    25381 cgcgtactac ctcttccatg gcggcactac gtgaaccctt aactaaaatc gtaattacct
    25441 gttgctcagc aatcagtgat ttaatacgcg cgataagcgc tgttttatct gcaaaatgtt
    25501 cgccaacgcc gctggcggtg ctgatagcat ggctttgttt acccacgctt aacacgcggt
    25561 caataccagc agcttttgcc gcctcgccca cctgtacatg gcaggcttcg ctttcagcgc
    25621 ccaattccgc catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct
    25681 ggactgctgc ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt
    25741 tttccgccag ttggatgggg aacagacggc ctggaacagc tttcagattt gccagccccg
    25801 ctttgatagc atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat
    25861 tcgcaatatt gtgacgccct ggaagcggca gcagaacatc gacgctacct gttggggttt
    25921 ggagggtaaa ttccgtaccg tgcgaagtca catggatgtt ggtggcggtg aaatcgctgt
    25981 tggcggcatt cggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca
    26041 gccagtcgtt gttgtcggca ttcataatgg cgataccgtt ttccggcagg ccgctaaaga
    26101 tttcgccttt cgcttttgcc acgccagcca aagaaccgaa accttccaga tgcgctgccg
    26161 ccaggttatt gaccagcgcc gcttccgggc gagtcagact cacggtccag gcaatttcgc
    26221 cctgatggtt cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcagca
    26281 gcgtcatcgg cacgccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc
    26341 actggcttaa aattgccgcc gtcatctctt taacagaggt tttgccggag gagcccgtca
    26401 gagcaaccac gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca
    26461 gacgcgtatc cttgacgatt aactgcggca gatcgatatc cagcggacgg ctgaccagta
    26521 gtgcgcccgc gccgccagct ttcgcctggt cggcaaaatc atgagcatcg aaacgttcgc
    26581 ctttcagggc aacaaacagg cagcccggcg tcagttttcg cgtgtcagtg gttacagcat
    26641 caagggtgat atctgcacct tgcagttcac cgttgagaat atcggtaagt tggctaaggg
    26701 ttacgctaat catgcaatca cccccagcag acgcgccacc gtgacgcgat cggagtagtc
    26761 cagacgctga ttgccaacaa tctggtaatc ctcatggcct ttgcccgcga ccagtaccac
    26821 atcattctct ttagcctgca taacggcgca agtcaccgct tcagcacggc cttccatcac
    26881 tttggcatgt ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggttc
    26941 ttcggtacgc gggttatcgt ccgttaccac cgccacgtca gcgaactctt cagcaattgc
    27001 gcccatcagt ggacgtttgc ctttatcacg atcgccacca cagccaaaga cgcaccacag
    27061 cttgcccgta cagtgcaggc gcgccgcctg taaggctttt tccagtgcat ctggcgtatg
    27121 cgcgtaatcc accaccaccg tcggtttgcc tggtgcagtg aacacttcca tacgtccgca
    27181 aaccggttgc agacgcgcgg cggttttcag cagctcagcc agtggatagc ccagcgccag
    27241 cagcgtcgcc agcgcgagca gcaggttgct gacgttaaaa gcgcccatca gatggctttc
    27301 aatttcgcca tcgccccaac ttgagctaaa gcgaatcgtc gcaccgctgt cgtgatagtt
    27361 cacgtcgatc gcttttaacc agcgtccgtg acaattcgga ttaatatgat cttccattga
    27421 taccgcaacc gcgtccggca gttttgccag ccagcggcgg cccacttcat cgtcggcgtt
    27481 aataatcgcc tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg
    27541 ttccatatca ccatgataat caaggtgatc gcggcttaag ttggtaaaga ccgacgccgc
    27601 aaattttaat gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca
    27661 aaaagtcgcg ccctgatcca ccagccccgc cagctcatgc tgaacatcga ctgccgaacc
    27721 ggtggtattt tctgtcggga tcactttccc cagcagaccg ttaccaacgg tgcccattac
    27781 cgcgctggtt tcgccaagca gttggctcca ctgcgccagc agctgggtcg tcgtggtttt
    27841 gccgttggtg cccgttacgc ctacaagacg taaattgtca gagggttcat ggtaaaagcg
    27901 gcccgccagt gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg
    27961 catttcacgg atttcaccgt cggtcgcctc atctttcgcc tctgcaataa tggcagccac
    28021 accttgcgct atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc
    28081 tacaaagaga tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg
    28141 cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg
    28201 tcccctcgcc ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct
    28261 cgatgttcat ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg
    28321 cgccgccgta gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg
    28381 gctgactcgc aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt
    28441 ccggcccaac ctttttcgcg gtaccggttt taatggcgat acgatagccc ttaatcgccg
    28501 ccttcacgcc gccaccaccc ggtagcgcca cgctttccat catatgcacc acggtgcgaa
    28561 caatggattc cgggaagact cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca
    28621 gtgggcgata aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg
    28681 ttaccattag cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt
    28741 tttgaggata taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc
    28801 caaaacgtga gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa
    28861 caccgacgtt actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca
    28921 cgtctttgat ttcatggccg ttaattcgat aaggaacggt attcagtacc gagttttccc
    28981 gcactacgcc acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gaacccggtt
    29041 caaacacgtc agtgatggta cggttacgca tcgcctcttt cggcgtgcca ctcagattgt
    29101 ttgggttgta tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca
    29161 gcacggcgct accagattca gctttgttaa aggccaccgc gttgttcagt tcgcgataaa
    29221 ccagcgcctg caggcgttca tcaatactca gcgccaggtt gtgcgccgcc tgactgtcgg
    29281 tggaagagat atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg
    29341 gctgcccggt aagccattta tcgaaactct tctcgacgcc ctcaatcccc tgactatcga
    29401 cgttagtaaa gccgatgagg tgagcggtca cttcgccgga tggataataa cggcgagatt
    29461 cttcacgcag atgaatcccc ggcagtttca gttttttgat gtactctgcc atgtcagggt
    29521 ttacctgacg cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggctgaaa
    29581 gctgatccag cggaatattg agtgcgttag ccagcgcctt ccagcgatca ccgacgctga
    29641 taccgccagc gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca
    29701 ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa
    29761 cgcgaagaga acgcatgtcg ccctctttta ccagcatatc cggagagata acttgtaacc
    29821 acgctacgcg tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa
    29881 aacgccaact gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg
    29941 ctgctttcat gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat
    30001 caacatgctg catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga
    30061 gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca
    30121 ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca
    30181 ccgccgtcaa aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat
    30241 cgtcaccgat aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt
    30301 tgcttagagc ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac
    30361 tgaactacgg gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag
    30421 tgctcgcagc tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc
    30481 cggaacttgc ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag
    30541 cgagtggaag ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt
    30601 tagcgcctgc tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt
    30661 ccgggtcgcg ggatgtttaa acttatcttt caccggcgta gcggcagcca cgacttccgc
    30721 cagttctttg gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat
    30781 gcgtttggca aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc
    30841 tgcggtttgt agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag
    30901 cggaccatcg cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac
    30961 gccaagatcg aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacatattc
    31021 gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc
    31081 aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg
    31141 cgagaggatc agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc
    31201 aggacggata ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt
    31261 ttccatcatt ttatagagac aagtcctgca gtcgctctga taagtctcca gtagccaact
    31321 gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa
    31381 acttgttgaa ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta
    31441 ctggcgcgat taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca
    31501 gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct
    31561 cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg
    31621 tgcaaaccat ttgaccggca gcgttctcaa gcagctgctc ccgataacgg gtaggcactg
    31681 ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac
    31741 cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa
    31801 agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata
    31861 tcagcgttgt ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa
    31921 gaaaaaatcc ctcatcgtca tcaccgtttt aaaatgacta actattttcc acagcaaata
    31981 taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc
    32041 cctgttgcgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgt
    32101 ttgttaaatc ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctatcgat
    32161 atcacttaat tattgtgaat ttcatctggt gaggtaatta acgggcaggt ttataagcat
    32221 ttatcaataa tagccaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt
    32281 actcaacgcg ccagagagaa attcacctga cgcgtatttt tgttcgcggc ttagctacgg
    32341 ctgagcacgc cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta
    32401 cgcggttcgt ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga
    32461 cgttgagcca ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag
    32521 gtggcaattg ccaggtcaga aggcagtttg ccgtcacgac gtagcgtgac atccatcacc
    32581 ccttgcaaca acgcaaacga cgttgtgaac agtgcctgcg gcatcggatg cgtttccagc
    32641 catttttcga ataactgggc agccgcctcc cgctcatagc tgttggcata aaggaaatgc
    32701 acttcgcgcg gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca
    32761 gaaagctccg gcagcgcgcc aagataaagt accgtctcgg cgggaaactt acgtaactct
    32821 tccgccagca tttcggcatc atcctgatcg gcaccaacca cgctggtgaa atgttcacga
    32881 tcgagggcgc ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa
    32941 ggatgctcag gtggcaacga cgtcgaaaca ataatggcat caacctgacg ctgcaaaaga
    33001 tgctcgatac aacgcatttc gttgtcgggc tgatcttccg agcaggcaat cagcagttga
    33061 taaccccttt gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc
    33121 tccagatcgg ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc
    33181 acggcgttcg ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg
    33241 tcgctcacac ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac
    33301 actcccgcca gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa
    33361 aaccatagcc attgtactgg tatgggttaa attcattaac atctaagcgc agaaaattaa
    33421 ctgcggcaac tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac
    33481 atgttgggct gcaaattgcg cattgagatc attctcaacg cattatttta tcgccgcgcg
    33541 aaagtccgac tacaccagag cgagcaactt ccacaatttt cgccacatcg cgaatcgatg
    33601 ctaaaaatgc atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg
    33661 tgacatcaat aatttgccca cggaatattt ccgtattacg tttaacttcg tcacgcccgt
    33721 agccgctggc ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccta
    33781 actcgctcac gcgtaataca tcgactagct tgtgtaattg cttttcgatc tgctcaagca
    33841 ctttttcatc gcccacggtc tggatggtca tacgcgataa tgtcggatcg tcagttggtg
    33901 caacggtcag gctttcaatg ttgtagcctc gctgggaaaa aaggccaatc acgcgggata
    33961 acgcgcctga ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc
    34021 cgttttgctt aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg
    34081 ctcactgcca tcgacggtga catcaacaaa caccaggcga ttattacgca cctgttccag
    34141 cgcctcgcta agtttgcttt ccagttcttg cggatgagca atctggatcc cgacgtgacc
    34201 ataggcttcc gccagacgaa cgaaatcggg tagagattgc atataagatt gtgaatgacg
    34261 gccggaatag atcatgtcct gccactgttt caccatcccc agatagcggt tattgagatt
    34321 cactaccagt accggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg
    34381 aatactgccg tcgcccgtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc
    34441 cagtgccgca ggtaaaccaa aacccatcgt gccgaggccg ccagagttga tccagcgacg
    34501 cggtttgtcg aatggataat aaagcgcagc aaacatctgg tgctgcccga catcggacgt
    34561 cacgtaagcg tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat
    34621 cttttcactg tgagagtcat atttcaggca ctgacgagcg cgccactgtt caatctgttg
    34681 ccaccagtcg cggatctcat ccagcggttg atggacggat tcttgcgaca ggagttcaag
    34741 catttgttcg aggacctggc gagcatcccc cacaatcggg atatcagcag tcacggtttt
    34801 agaaatggaa gtaggatcaa tatcgatatg cagaaccgtg gcatttgggc agtacttcgc
    34861 cagattgttc gtcgttcggt catcaaaacg taccccgacg gcaaaaatca catccgcgtt
    34921 atgcatcgtc atattagctt cgtaggtgcc gtgcattccc agcatgccca gtgcctgacg
    34981 atgcgttgcc ggaaacgccc ccagccccat taatgaggaa acaacgggca ggttcaacgc
    35041 ctccaccgtt tcttttagct gctgatgaca gcccgccgtg attgccccac cgcctacata
    35101 gacaaccggt ttttttgccg ccaccagcgt ttgcagagca cgcttaattt gccctttatg
    35161 cccggaggtc gtgggattgt aagatcgcat actgaccgac tccggccaga cgtagggtaa
    35221 tttgttcgcc ggattaagaa tatctttggg taaatcaacg accacgggcc cagggcgacc
    35281 acttgccgcc agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac
    35341 cagaaaactg tgtttaacca ccgggcgaga aatccccacc atgtcgcact cctgaaaggc
    35401 atcgtagcct atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc
    35461 catataagcg gtggcaatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag
    35521 cacgacgccg acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ccgccgcctg
    35581 ctcatgacga actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc
    35641 aaggaccgcg cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg
    35701 gacgaccatc tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa
    35761 aatgtgtaaa acacattaac cgtccgatct tgcttaagca att
//
LOCUS       DARNVM010000027        35743 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.027, whole genome shotgun sequence.
ACCESSION   DARNVM010000027 DARNVM010000000
VERSION     DARNVM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.027"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(243..956)
                     /gene="lpxT"
                     /locus_tag="SI621_001285"
     CDS             complement(243..956)
                     /gene="lpxT"
                     /locus_tag="SI621_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HEI4064022.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(995..1981)
                     /gene="yeiR"
                     /locus_tag="SI621_001286"
     CDS             complement(995..1981)
                     /gene="yeiR"
                     /locus_tag="SI621_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HEI4064023.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHPHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNIAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(2099..3565)
                     /locus_tag="SI621_001287"
     CDS             complement(2099..3565)
                     /locus_tag="SI621_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEI4064024.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSMLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQTIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(3788..4360)
                     /gene="yeiP"
                     /locus_tag="SI621_001288"
     CDS             complement(3788..4360)
                     /gene="yeiP"
                     /locus_tag="SI621_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HEI4064025.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4515..4769
                     /locus_tag="SI621_001289"
     CDS             4515..4769
                     /locus_tag="SI621_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEI4064026.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEILTAP"
     gene            complement(4766..5947)
                     /gene="setB"
                     /locus_tag="SI621_001290"
     CDS             complement(4766..5947)
                     /gene="setB"
                     /locus_tag="SI621_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HEI4064027.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6315..7445
                     /gene="fruB"
                     /locus_tag="SI621_001291"
     CDS             6315..7445
                     /gene="fruB"
                     /locus_tag="SI621_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HEI4064028.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7445..8383
                     /gene="fruK"
                     /locus_tag="SI621_001292"
     CDS             7445..8383
                     /gene="fruK"
                     /locus_tag="SI621_001292"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEI4064029.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8400..10091
                     /gene="fruA"
                     /locus_tag="SI621_001293"
     CDS             8400..10091
                     /gene="fruA"
                     /locus_tag="SI621_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HEI4064030.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDVVAKAA"
     gene            10515..11456
                     /locus_tag="SI621_001294"
     CDS             10515..11456
                     /locus_tag="SI621_001294"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HEI4064031.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVVDCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVASWFHQHGLN
                     RLVLSMGGNGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11444..12382
                     /gene="psuG"
                     /locus_tag="SI621_001295"
     CDS             11444..12382
                     /gene="psuG"
                     /locus_tag="SI621_001295"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEI4064032.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPIVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAAIAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12476..13726
                     /locus_tag="SI621_001296"
     CDS             12476..13726
                     /locus_tag="SI621_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEI4064033.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13794..14492)
                     /gene="yeiL"
                     /locus_tag="SI621_001297"
     CDS             complement(13794..14492)
                     /gene="yeiL"
                     /locus_tag="SI621_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HEI4064034.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVSLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14622..15563
                     /gene="rihB"
                     /locus_tag="SI621_001298"
     CDS             14622..15563
                     /gene="rihB"
                     /locus_tag="SI621_001298"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HEI4064035.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFGPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15663..16913
                     /locus_tag="SI621_001299"
     CDS             15663..16913
                     /locus_tag="SI621_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEI4064036.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKRISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALT
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16969..18057)
                     /locus_tag="SI621_001300"
     CDS             complement(16969..18057)
                     /locus_tag="SI621_001300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEI4064037.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLIYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18060..18917)
                     /gene="nfo"
                     /locus_tag="SI621_001301"
     CDS             complement(18060..18917)
                     /gene="nfo"
                     /locus_tag="SI621_001301"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEI4064038.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18991..20040)
                     /locus_tag="SI621_001302"
     CDS             complement(18991..20040)
                     /locus_tag="SI621_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEI4064039.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            20139..21020
                     /locus_tag="SI621_001303"
     CDS             20139..21020
                     /locus_tag="SI621_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4064040.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21225..22694
                     /gene="lysP"
                     /locus_tag="SI621_001304"
     CDS             21225..22694
                     /gene="lysP"
                     /locus_tag="SI621_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HEI4064041.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22986..24965
                     /gene="cirA"
                     /locus_tag="SI621_001305"
     CDS             22986..24965
                     /gene="cirA"
                     /locus_tag="SI621_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HEI4064042.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRAGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEDGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24997..25833)
                     /gene="yeiG"
                     /locus_tag="SI621_001306"
     CDS             complement(24997..25833)
                     /gene="yeiG"
                     /locus_tag="SI621_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEI4064043.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            26091..26759
                     /gene="folE"
                     /locus_tag="SI621_001307"
     CDS             26091..26759
                     /gene="folE"
                     /locus_tag="SI621_001307"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEI4064044.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26776..27933
                     /locus_tag="SI621_001308"
     CDS             26776..27933
                     /locus_tag="SI621_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HEI4064045.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLMLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            28075..29115
                     /gene="galS"
                     /locus_tag="SI621_001309"
     CDS             28075..29115
                     /gene="galS"
                     /locus_tag="SI621_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HEI4064046.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29395..30393
                     /gene="mglB"
                     /locus_tag="SI621_001310"
     CDS             29395..30393
                     /gene="mglB"
                     /locus_tag="SI621_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HEI4064047.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30454..31974
                     /gene="mglA"
                     /locus_tag="SI621_001311"
     CDS             30454..31974
                     /gene="mglA"
                     /locus_tag="SI621_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HEI4064048.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31990..33000
                     /gene="mglC"
                     /locus_tag="SI621_001312"
     CDS             31990..33000
                     /gene="mglC"
                     /locus_tag="SI621_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HEI4064049.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33071..34306)
                     /gene="preA"
                     /locus_tag="SI621_001313"
     CDS             complement(33071..34306)
                     /gene="preA"
                     /locus_tag="SI621_001313"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEI4064050.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQIRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHSVTL"
     gene            complement(34300..35538)
                     /gene="preT"
                     /locus_tag="SI621_001314"
     CDS             complement(34300..35538)
                     /gene="preT"
                     /locus_tag="SI621_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HEI4064051.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTFTLDQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         8696 a         8758 c         9384 g         8905 t
ORIGIN      
        1 agttcgagat tacccgattg gtgcggtctt ggcgagcggt tttacatgat tttttataag
       61 aaaaagtgat acagaaagtt aataaacggg attggtcaat taaagtacga ttgagtgaaa
      121 cgcatgcaat ccattcattt acttagagaa tgatttttgc gcgcgatgga tgaaagcacg
      181 gtgcgcatca tcaggattat cctcactata aaaataaccc tgatgatgtt aattactgtg
      241 agttatttgt tttggaaatg tttgtttttt cccggtagtg atttgtcaaa aaaggtgatt
      301 aatcgatcac ttaatggcgt cagcaacacc cagggcaaac cgatcaatat cacggtcatc
      361 gaaccgacaa tgatgtcagt aaaccagtgt gcgccaatca ttactctggg aaacgcaaaa
      421 accacaaaaa taataagggc gataaggcct gcaactttgc cgaaataacg ccacatgaat
      481 gccgaaaaaa taagcagcat cataccatga tcgccgggaa agctatctcg tgaggcatct
      541 ttagtgggaa cagagagcag ttcgctgacg cggttaatat cggtaaaagt caatgttggg
      601 ctggcccgtt ttacaggaat taatgcctga cccagctggt ttaataccac tgcagttaat
      661 agcatgacca gaccaataat cacgatacgt cgtctgcctg gggcgttttc tttcagccag
      721 aaactcagca tcaacattcc catcgccagc agagaacaac cgtcgaaagc gcgattgttg
      781 gtcaatgcaa ccagccacaa aaaggcctta ctttcgacca gtttctgatt aaagaaataa
      841 aaaatatccg catcaatcgg caaccagaat ccatgattaa cgggaatata ccaggaaaga
      901 aacagcgcga ggccgacaat attcaacaac actatttgcg gcaaattttt aatcatattt
      961 tctctaatta tcttactgaa aaacgcaggc aaccttacgc ggtagtcgct aaacgaagct
     1021 tcaacagcgc gctctgcaag gcattccagt cagcttcgct gctggaaatc agctcaatac
     1081 ggctgtccgg tggcgcaatg ttttgcgttt caatgtgcag gtcatcgccc tgacggttga
     1141 ttcgcaccag cccttcggga atacgcagca cgcctttgac gcgttccacc ggtgcaagtc
     1201 gcgcccattc cagaatgcca atggtgtcga ataccgtatc agcgtcgaat atccagccgc
     1261 aggcctgata tccttgcccg ctgttcagac tgcgacgcca gcgttgatgc tctggcaggc
     1321 ttaacgctgc taaccctttt ttcacgccat gcggatgaga atgcgcggcg ctggcgggca
     1381 actcggctaa attgcgacgc ggcaaatcca gaagatgacc gtcaactttc ccatgctcac
     1441 tgtgaattaa ttgccgatcg ccaccatttt gctgccacca gcgctgtagc gcttgctcgc
     1501 tttcaggcgt cgcacggtcg gatttattgg cgacaatgat gtctgcggca gccagctggt
     1561 cacggaagtt ttcattgctg gcgctttttt cgtccagcag caggcgtgga tcgagaatgc
     1621 acaaggtggc gcgcagatct atccacggtt catagactgg cgcggttaag agatcgagga
     1681 tctgtttcgg atgccccagt ccggtcggct ctatcaacaa gcggtctggt tttccctgac
     1741 gcagtaaggt attcaatcct acctgcatgg gtaaaccatt aacgcagcac atgcagccgc
     1801 cggggatctc tttcaacaat gcgccgctat cggcgagcaa agcaccatca attccgactt
     1861 ccccaaattc attaaccagt accgcccatt tttcgttggg atctttatgg gctaacagat
     1921 gaagaatcga cgtggttttc ccgctgccga gaaaaccggt gatgaggttg gtcctggtca
     1981 caacggctcc gcaatactct taaaagttaa ctgagcgtta atcctggcta aaaacggcag
     2041 aaaagagaag gggagagcgg acgaaggcga gcttcatccg ctttaaccgc agaaatcgtt
     2101 aaattttgag ggtgtttaat agcgcctgat gcgcgccgaa ttgtgcgatt tgttgccagg
     2161 cttgttcgat ggtctgcaca aaatgcgggt tatctggcag atcatcaccg aaaacttcac
     2221 gcagggaaag cagggcggtg acgcgttgtt cactgctgct gcccgcaaca agttcgcgaa
     2281 ttttatcgct aagcggatcg cgaacatcaa tggcatttcc ggcatcatca acgccgctga
     2341 cgtaacgcat ccagcctgca acgcctaatg ccagcatcga ccagtccgtt tcgcgcccca
     2401 gatgtatgcg aatacctgcc agcatgcgtt gcggtaattt ctggctacca tccattgcga
     2461 tttgccaggt cttatgtttc agcgccggat tagcaaaacg tgcaattaac ttatccgcat
     2521 attgtgttaa atcgacatct ttaattcgca gtgttggcgc ttgctcatcc agcattaatg
     2581 ttctggcggc atggcgaaat gcgcgatcct gcatgcaatc actgatatgg gcgaatcctg
     2641 agaggtaacc cagataagcg agaaaagagt ggctgccatt aagcatacgc agtttcatct
     2701 cttcccatgg caggacatca ttcaccattt gtacacctgc gacttcccag gcaggacgcc
     2761 cagcgacgaa gttatcttcc accacccact ggataaacgg ttcgcagcta atcgcgcagg
     2821 gatcattcac ccccagatgc tggctgattt ccgctagtga ttcgtcggtt gcagccggaa
     2881 caatgcggtc gaccatggtt cccggaaaac tgacgtgttc ttttatccac ccggcgagtt
     2941 ctggtgaacg tttttccgcc atccccagca ccgcgttttt caccacatgg ccattgtcgg
     3001 gaatattgtc gcaggagagt acggtaaacg gtgcaaggcc gcgttcacgg cgtcgtttca
     3061 gtgcttcgac gagaataccc ggcgcggagt gaggttcttc agggttttgt agatcgtgaa
     3121 taatccgcgg attgctggtg tcgagtgcac cggtggccgg gtcaatacaa tagccttttt
     3181 cggtaatcgt cagggaaaca attgccacct gtggctcgca aaatttctca ataattgccg
     3241 ctaaggaatc cagttttgca ttaaggcatt cgtgaacggc accgacaatt atcgcctgat
     3301 tgccgtccgc ccctttctcc agcacggtat ataaatggtt ctgtgcgcgg agctggctca
     3361 tcagttgatc accgctgaac aagctgattt cacagatccc ccagtcaccg ccctgggcat
     3421 tcagcacacg atcggtcagt aacgcctgat gtgcgcggtg aaaggctcca aagccaaaat
     3481 gaacgatacg tgattgcaac tgctggcgat cgtagcgtgg agcgtgtaca tgatgcggga
     3541 gcgtaacgga ggcgattgta ttcataggag tcacgtctta gtaacgatta attaacaatg
     3601 acagtgtaaa gttatatgac agcaattgaa attggtctgg attgttaatg cgatgaagtg
     3661 tgagggcgat caatcaacga gtgaaaattg aggacgtaag gtgttcagga agcgcaaaat
     3721 agaagattgg tataataaat taaaaaaggg acagcctgag ctatcccttt tctgcatgcg
     3781 gctgaagtta gtcagcacga cccatataac ggcgttcttc gatatggata cgaatttttt
     3841 cgcccgggct taagtattct ggtacctgaa tcaccagacc agtgctcaat gtcgccggtt
     3901 tgttacgggc gctggcagat gcccctttga tgcctggtgc ggtttcaacg atttccagat
     3961 caacggtctg cggcagctca agcgccaaca gttgaccgtc ccaggtcagc acctgcatgt
     4021 ccggcatgcc gccttctggc ataaacagca actcttcttc aatctgatct ttggtgaagg
     4081 tatacggggt atagtcttct ttatccataa agacatattc gttaccatcg acataggaga
     4141 agtcaacgta acggcgggtc agcgtcacgg tgtcaacgat gtcatcacct ttgaaacgct
     4201 cttctacttt cagcccggta cggacatcag aaaaacgcat tttgtacagc gtagcggcac
     4261 cgcgggcagt gggcgactga atatcaatat ccttaaccag cagcagtttg ccgttgtaat
     4321 tcagtaccat acctttttta atttcgttcg ctcttggcat cgaaaaattc ctgtagtcga
     4381 aaatgtcaaa aatatcgcga caaagtactc gcgacgggcc tttcgggcaa gtggtattcg
     4441 cacttttgct ggtgcaaaaa aggtggtact gtgcgcgctc atcaatccgg tggttaacct
     4501 taagagaaca acgtatggaa tgccgtccgg gttgcggggc gtgttgcact gccccttcaa
     4561 tttccagccc tattcccggt atgccagatg gcaagcccgc caatacgccc tgcattcagc
     4621 ttgatgaaca gcaacgctgt aaaattttca cctcgccact gcgaccgaaa gtctgtgcag
     4681 gcttacaagc cagcgctgaa atgtgcggta actcgcgtca gcaggcgatg acctggctta
     4741 tcgatctgga aatcctgacc gcgccttaaa catctttaat ccgtagtaag caaaacagag
     4801 tggcgataat catcaccatc gcaaaccaga acacagcgtg ataattccag atctcggcga
     4861 cgatgcccgc cactgatcct gcgatgatcc agcccacgcg cgaagtgttg gtatagagcg
     4921 tggtggctga acctgcctga ccgggcatca gatcctgaaa atagagcatc ccgatgccgc
     4981 ccagaatgcc aataaaaata gcatttagca gctgcaagcc caacagaatg acaggtgaat
     5041 gcgccatcag cattcctgcg taaaaacaga cgccacccac ggcagcaacg cgcattaaga
     5101 aacgcttacc cagacgtttg gcgaaatatc cggcaatcaa catcgtcggg atttccagcc
     5161 cggcggcggt ccccatcatc acaccggcca gtttctcggg aagatgcagt tcgttgataa
     5221 taaatagcgg catgttgatg atgtacaggc tgttcgagcc ccacatcaat gtacaaatga
     5281 caaacagcag cagcgtatcg cgacgattac ggcgcggcgc ttcaaccgtg ccggtcgcca
     5341 gcggaagctc tttttgcatc gatggtaaaa acagccacac catcacgccg caaacaataa
     5401 acgctaccgc tgcgctcaga tacattaccg taaagctgaa acccatcgct aaggcataag
     5461 ccagcggcgg gccaatgacc catgccagtg aaacctgagc gcgtaaaaaa gagctgaaca
     5521 tcaccgcttc acgtccggtt ttgtcagcat gttcacgggc gagggcaaac atttgcgggt
     5581 tagcagtcga gccaaagctg ctaagaaaga cgccaacgaa tagcaaaaca aagtagttgc
     5641 gattccaggc aaaaagagtg caggccagca cgcctaacag gcagcaaaag acaatcagcg
     5701 atttgcgatc gccgcgctta tcagagcgcc cggcgagaaa ctgacttacc agaatcccaa
     5761 tgacagcgct gccggtaaag aagaatccca ccatcgctgg acgggcatgt acttcatcgg
     5821 taagaaaaat gctgagtgtc ggggtttgca gagcgcccgc aataccggtg agaaaagcaa
     5881 cgattaaaaa cgccgtcgag gtcaggtcaa acgatttcgc gctggagact gcgggggagt
     5941 tatgcatgct ggtcgatcac tggaagagga agacgcggag tttacgctgc ttgtcagaaa
     6001 ataaacagac agcgaaatca aaagcgccac gaataatcaa aatgccattt cactgttgct
     6061 gattggctgc tgacgctgct gaaatgaaat tgctgataac ggattttccc atcagcaatt
     6121 aggaaaaatg acaaaaaatt gtgcagcaca tcaaactttt gctcataact ttacggcttt
     6181 ccttgcgtgc tgaaacagaa gtattatgct ttcttgaaac gtttcagcgc gatcttgtct
     6241 ttaaccctaa gccaggttgg cgcttttttt ctcatagaag ctgaatcgtt tcaattcagc
     6301 aagagaggag aactatgttc cagttatccg tacaggacat ccatccgggc gaaaaggccg
     6361 gagacaaaga agaggcgatt cgccaggtcg ctgcggcgct ggtgcaggcc ggtaatgtag
     6421 ctgaaggcta cgtcaatggc atgctggcgc gcgaacagca aacctctacg ttcctcggca
     6481 atggtattgc tattccacac ggcactaccg acacccgcga tcaggtgctg aaaaccggcg
     6541 ttcaggtatt tcagttcccg gaaggcgtca cctggggtga cggccaggta gcgtacgtgg
     6601 cgatcggtat tgctgccagc tcggatgaac atctgggcct gctacgccag ctgacccacg
     6661 tactgagcga tgattccgtt gctgaacaac tgaagtcagc aacaacagca gaagaacttc
     6721 gcgcattgct gatgggcgaa aagcagagtg agcagctgaa gctcgacaac gaaatgctga
     6781 cgctggatat cgtcgccagc gatctgctga ctcttcaggc gctgaacgct gcacgtctga
     6841 aagaagcggg ggcagttgac gccactttcg tcaccaaagc catcaatgaa caaccgctga
     6901 acctcggaca gggtatctgg ctgagcgata gcgccgaagg caatctgcgt agcgcgattg
     6961 cggtaagccg tgcggcaaat gcttttgatg tggacggcga aacggcagcc atgctggtga
     7021 gtgtggcgat gaatgacgat cagcccattg cggttcttaa gcgtctcgct gatttgttac
     7081 tcgacaataa agctgaccgc ttgctgaaag cggatgcggc aacgttgctg gcgctgctga
     7141 ccagcgatga tgcgccgacc gacgacgtgt taagcgcgga gtttgtggtg cgcaatgaac
     7201 acggcctgca tgctcgtcca ggtaccatgc tggtcaatac cattaaacaa tttaacagtg
     7261 atattaccgt gacaaacctt gatggcaccg gcaaaccggc aaacggacgt agtctgatga
     7321 aagttgtggc acttggcgtt aagaaaggtc atcgcctacg ctttaccgcc cagggtgcag
     7381 atgctgaaca ggcgctgaaa gccattggcg acgctatcgc tgctggtctt ggggagggcg
     7441 cataatgagc agacgtgttg ctactatcac ccttaatccg gcttatgacc ttgttggttt
     7501 ctgcccggaa attgaacgcg gcgaagtgaa cctggtgaaa accaccggtc tgcatgcggc
     7561 gggtaaaggc atcaacgtgg ccaaagtatt aaaagacctg ggaattgatg tcaccgttgg
     7621 cggcttcctc ggtaaagaca atcaggatgg ttttcagcaa ctgttcagcg agctgggcat
     7681 tgccaaccgt ttccaggttg tacaggggcg cacccgaatt aacgttaagc tgacggaaaa
     7741 agacggcgaa gtgaccgact tcaacttctc gggttttgaa gttacccccg ccgactggga
     7801 acgctttgtg actgattctc tgagctggct cggtcagttc gatatggtct gtgtcagcgg
     7861 aagcttaccg tcaggcgtca gcccggaagc gttcaccgac tggatgactc gcctgcgtag
     7921 tcagtgtcct tgcattatct ttgatagtag ccgtgaagcg ttagtagcag gtttgaaagc
     7981 ggcaccgtgg ctggtgaaac ctaaccgccg cgagctggaa atctgggcag gccgtaaact
     8041 gcctgaaatg aaagatgtga ttgaagctgc acatgcgcta cgtgaacaag gtattgcgca
     8101 tgttgttatt tcactgggtg ccgaaggcgc gctttgggtt aatgcctccg gcgaatggat
     8161 cgccaaacca ccgtcagtcg atgtcgtaag caccgttggc gcaggggatt ctatggttgg
     8221 tggcctgatt tatggcttgc tgatgcgtga atccagtgaa cacacactgc gtctggcgac
     8281 agctgttgca gccctggcgg taagtcaaag caatgtgggt attaccgatc gtccgcagtt
     8341 ggccgcaatg atggcgcgcg tcgacttaca accttttaac tgacagcagg agaggcataa
     8401 tgaaaacgct gctgattatt gacgctaatc tcggtcaggc acgcgcctat atggcgaaga
     8461 ccctgctggg cgcggcggcg cgaaaagcaa aactggaaat catcgacaat ccgaacgacg
     8521 ctgaaatggc gattgttctc ggtgattcca tcccgaacga cagcgcgctg aacggtaaaa
     8581 atgtctggct gggcgatatt tcccgggcag ttgcgcaccc tgagctgttc ctgagtgaag
     8641 ccaaaggcca tgcgaaacct tacactgcgc cggtcgctgc gacagcaccg gttgccgcca
     8701 gcggtccgaa acgcgtagtt gcggtgactg cttgcccgac tggcgtagca cacaccttta
     8761 tggcggctga agccattgaa accgaagcga aaaaacgtgg ctggtgggtg aaagttgaaa
     8821 cccgtggttc ggttggcgcg ggtaatgcaa tcactcccga agaagtcgca gcagcggatc
     8881 tggtgattgt ggcggcagat atcgaagtgg atctggcgaa atttgctggt aaaccgatgt
     8941 atcgtacctc taccggtctg gcgctgaaga aaaccgcgca ggaactggat aaagcggttg
     9001 ctgaagcaac gccgtatgaa ccggcgggca aaactcaaac agcgaccact gaaggtaaga
     9061 aagagagtgc aggcgcttac cgtcacttgc tgacgggcgt ctcttacatg ctgccgatgg
     9121 tcgttgcagg tggtctgtgt atcgcgcttt cttttgcttt tggtatcgaa gcgtttaaag
     9181 agccgggtac gttggctgcg gcgctgatgc agattggtgg tggttcagcc tttgcgttga
     9241 tggtgccggt actggcaggt tatattgcct tttccattgc cgatcgtccg ggtctcacgc
     9301 cgggtctgat tggcggtatg ctggcggtca gcaccggttc tggcttcatt ggcggtatta
     9361 ttgcgggctt cctggctggt tacattgcga agttaatcag tacgcaattg aaactgccgc
     9421 agagtatgga ggcgctgaaa ccgattctga tcattccgct aatttccagc ctggtggtcg
     9481 gtctggcgat gatctacctg atcggtaaac cggttgctgg cattctcgaa gggttgactc
     9541 actggctgca gaccatgggg actgcgaatg cggttctgct gggggcgatc ctcggtggca
     9601 tgatgtgtac tgacatgggc ggtccggtaa acaaagcagc gtacgcattc ggtgtgggtc
     9661 tgctgagtac tcaaacctat ggcccgatgg cggcgattat ggcggcaggt atggtgccac
     9721 cgctggcaat gggcctggca acaatggtgg cgcgtcgcaa attcgacaaa gcgcagcagg
     9781 aaggtggcaa agccgctctg gtattgggac tgtgcttcat ttcggaaggt gcaattccgt
     9841 ttgctgctcg tgatccgatg cgtgtgctgc cgtgctgtat cgtgggtggg gcgctgactg
     9901 gcgcaatctc aatggcgatt ggtgcgaaac tgatggcacc gcacggtggt ctgtttgttc
     9961 tgctgatccc tggcgctatt acgccggtac tgggttacct ggtagccatt attgccggta
    10021 cgctggtggc gggtctggcc tatgccttcc tgaaacgtcc ggaagtggac gtagtagcga
    10081 aagcagcgta ataaaaggtg tgttacaggg cagaaatttc ctgccctgta acatttgatg
    10141 cgcgcaaaga gaatgaccgg tttgtgaatg taattttatg aatttaaatg gttttatctt
    10201 ttagtagaaa cctgaaactt tcatcaaaca ctaaggttat ttcctcaagg gcagattaat
    10261 ttctgccctt ttttattgtt cctgttctgt gcatcccgtc acaaattccc cgtttttgta
    10321 attcaggcat ttaatcgcgt tttgtgagcc tgctcaaatc tgcccatttt gcctctctgg
    10381 cggtaattta ctttacgtaa aacatgcata ctatgagcac atgtttaaat atgaacacat
    10441 gtttaaaccg tctttgcagt atcaatattc ttgtggattg acagggttgg taatttttta
    10501 ccagaggacg tgctatgcgc gaaaaggatt atgtcgtaat tataggttcg gcgaatattg
    10561 atgtcgccgg atattcacat gaatcattaa attatgcgga ttcaaatcca ggtaaaataa
    10621 aatttacgcc tggtggcgtc gggcgcaata ttgcacaaaa cctggcgttg ctggggaaca
    10681 aagcctggct actgagcgcc gtaggcagtg atttttatgg tcaatcgctg ctaacgcaaa
    10741 ccaatcaatc tggcgtttat gtcgataaat gcctgatagt gccgggagaa aatacgtcaa
    10801 gctatttatc attactcgat aataccggtg aaatgctggt tgctataaat gacatgaata
    10861 ttagcaacgc tattacagct gaatatctcg cacagcaccg tgaatttatt cagagggcaa
    10921 aggtcattgt cgtagactgt aatatcagtg aagaggcact ggcatggatt ctggataatg
    10981 ccgccaatgt acctgtattt gtcgatccgg tttcggcatg gaaatgtgtc aaagtgcgcg
    11041 accgtctaaa tcagatccac accctgaagc caaaccgcct tgaagcggaa accctgagtg
    11101 ggattgcttt gtcagggcgt gaagatgtgg caaaagttgc ttcctggttc catcaacatg
    11161 gcctgaaccg actggtattg agcatgggcg gcaacggcgt ttattacagt gatatcagcg
    11221 gtgaaagtgg ttggtctgcg ccgatcaaaa ccaatgtcat taatgttacc ggagcgggcg
    11281 atgccatgat ggcgggactt gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg
    11341 ttcgtttcgc acagggatgt tcgtcaatgg cgctctcctg tgaatacacc aataaccccg
    11401 atttatcgat tgccaacgtt atatcgttag tggagaacgc agaatgtctg aattaaaaat
    11461 ttcccctgaa ttattacaaa tttccccgga agtgcaggac gctttaaaaa acaaaaaacc
    11521 gatcgtggcg ctggaatcga ccattatttc tcacgggatg ccgttcccac aaaatgccca
    11581 gaccgcaatt gaagttgaag aaactattcg taaacagggc gctgtacctg ccactatcgc
    11641 cattattggc ggcgtgatga aagtgggttt aagcaaagaa gaaattgaat tgctgggtcg
    11701 tgaagggcat aacgtgacca aagttagtcg tcgcgattta ccttttgttg ttgccgccgg
    11761 aaaaaatggc gcaaccaccg ttgcttcaac gatgattatt gcggcgattg ccggaattaa
    11821 agtatttgcc accgggggaa ttggtggtgt gcatcgcggg gcggaacata ccttcgatat
    11881 ttctgccgat ttgcaagaac tggcgcacac caatgtcacc gtcgtttgtg ccggggcgaa
    11941 atctattctc gatttaggct taactaccga atatttagaa actttcggtg taccgctaat
    12001 tggctatcag accaaagcgc tgcctgcctt tttctgccgt accagcccat ttgaagttag
    12061 tattcgcctt gatagtgcca cggaaatcgc tcgtgcaatg gcggtgaaat ggcaaagcgg
    12121 tctgaacggt ggcctggtgg tggcaaatcc gatcccggaa cagtttgcga tgccggaaca
    12181 cactatcaat gcggcgatcg atcaggcggt agctgaagct gaagagcagg gcgttattgg
    12241 taaagaaagc acaccgttcc tgctggctcg tgttgctgaa ctgactggcg gtgacagcct
    12301 gaaatccaac atccaactgg tgttcaacaa cgccattctg gcgagcgaaa ttgccaaaga
    12361 ataccagcgt ctcgcgggtt aatttctgac gggcagaaag ccgtcgcccg tactgatcat
    12421 acataccaaa acctggcgct gacgccgggt ttactggcat gaagggaaaa tcattatgga
    12481 tataatgaga agtgttgtgg ggatggtggt gttactggca atagcatttc tgttgtcagt
    12541 gaataaaaag agcatcagtt tgcgcacggt tggagccgca ctgctgctgc aaatcgctat
    12601 tggtggcatc atgctctact tcccaccggg aaaatgggca gtagaacagg cggcattagg
    12661 cgttcataaa gtgatgtctt acagtgatgc cggtagcgcc ttcatttttg gttcgctggt
    12721 tgggccgaaa atggatgtcc tgtttgacgg tgcgggtttt atcttcgcct ttcgcgtact
    12781 tccggcgatt attttcgtta ctgcgctcat cagtctgctg tactacattg gcgtgatggg
    12841 gctgctgatt cgcatccttg gcagcatttt ccagaaagcc ctcaacatca gcaaaatcga
    12901 atcttttgtt gcggttacca ctattttcct cgggcaaaat gagatcccgg cgatcgttaa
    12961 accgtttatc gatcgcatga atcgcaacga gttgtttacc gcaatttgta gcgggatggc
    13021 gtccattgct ggttcgatga tgattggtta tgccggaatg ggcgtaccaa ttgactacct
    13081 gttagcggca tcgctgatgg cgatccctgg cgggattttg tttgcacgta ttcttagccc
    13141 ggcaaccgag ccttcgcagg tcacatttga aaatctgtcg ttcagcgaaa cgccgccaaa
    13201 aagctttatc gaagcagcgg cgagcggtgc gatgaccggg ctgaaaatcg ccgctggtgt
    13261 agcgacggtg gtaatggcgt ttgtcgcaat tattgcgctg attaatggca ttatcggcgg
    13321 aattggcggc tggtttggtt ttaccaatgc ttctctggaa agtatttttg gctatgtgct
    13381 ggcaccgctg gcgtggatca tgggtgtgga ctggagtgat gccaatcttg cgggtagcct
    13441 gattgggcag aaactggcga ttaacgaatt cgtcgcttac ctgagtttct ccccatacct
    13501 gcaaacgggc ggcacgctgg aagtgaaaac cgtggcgatt atctcctttg cgctttgtgg
    13561 ttttgctaac tttggttcta tcggtgttgt cgttggggca ttttcggcta tttcgccaaa
    13621 acgtgctccg gaaatcgccc agcttggttt acgggcgctg gcagcagcaa cgctttccaa
    13681 cctgatgagc gcgaccattg ccgggttctt tattggttta gcttgagttt cattgccgga
    13741 tgtacgcgct tattaagcgt tgcatccggc aatcgcgcta ttgcaaataa tttttactgc
    13801 atcatcccgg agaatttatt ctccgggtcc atctccagtg ccagccctga caactgcttt
    13861 ctgtttttaa ttagatatcc tttcttgctt ttaattaata aaccgtcgtg aatgaattgc
    13921 gcgagaacat ataaaagatg tcgataagaa acgcctaaat actctgccgc ttgcgtatgc
    13981 ttttcgtgat aaagatcacc ttcctgcgag agtaatataa aagctgccag gcgattcact
    14041 aacggaaatg attgattctg agttaaagaa acaatattgc gataattttt atggcttaaa
    14101 ctaacgcaga gttttcgtaa aaatagtgtg tcgtttaata acagcggacg gtaatgtttc
    14161 atagggagcg caaggcacca acactcttca atagcctgca ccgcacgcgg ttcatggtct
    14221 ttatctatta actcaatctc accaataaaa cagggggcgg caaagaaatc gatcagcgac
    14281 acgcgaccat tagcaagcgt ggcgtaaagc ctggcgcggc ctcgcgtcag gtaaaacagc
    14341 caggacggct gttgcccttc ctgcacaatg tagtcacgcg ctaaaaaatg aaacagccgc
    14401 gtatcggctg aaacatccgt tgaaaagcaa tccttaaacg cggattcact catcaattgc
    14461 tgcttaagat cattattgtg gatttctttc acgatgttta tccgctatga gatttctcat
    14521 atttagcata cgtacgctgt tgttaagatt caatcatcgg cccggtcgaa ttttctttgc
    14581 tgtagaaaaa ataacgcaac tggaaacaga ggaaataaac aatggaaaag agaaaaatta
    14641 ttctggattg tgatccgggt catgatgatg ctattgcaat gatgatggca gcgaaacatc
    14701 cggcaataga tttattaggc atcaccattg tggcgggtaa tcagacgctt gataaaacat
    14761 taattaatgg cctgaatgtt tgccagaaac tggagattaa tgttccggtt tatgcgggga
    14821 tgccacagcc cattatgcgt caacaaatcg ttgccgataa tattcacggt gaaaccggac
    14881 ttgatggccc ggtattcggg ccgctgaccc gccaggcaga aagcactcat gcggtgaaat
    14941 atatcatcga taccctgatg gcaagtgatg gcgatatcac tctggtgccg gttggtccac
    15001 tttcaaacat cgcggtggca atgcgtatgc aacccgcgat cctgccgaaa atccgtgaaa
    15061 ttgtgctgat gggcggcgct tacggtactg gcaactttac gccatccgcc gagttcaaca
    15121 tctttgctga cccggaagcc gcacgcgtgg tgttcacctc cggcgttccg ttggtgatga
    15181 tgggcctcga tctcaccaac cagactgttt gcaccccgga cgtgattgct cggatggaaa
    15241 gggtaggcgg ccccgccgga gagctgttca gcgacatcat gaatttcact ctaaaaacgc
    15301 agttcgaaaa ctacggcctg gctggcggcc cggtgcatga cgccacctgc atcggttatc
    15361 tgattaaccc tgatggcatt aaaacccagg agatgtacgt cgaagtggac gtcaacagtg
    15421 gcccttgcta tgggcgtact gtctgcgacg agctgggcgt tcttggcaag cccgctaata
    15481 ccaaagtcgg catcactatt gataccgact ggttctgggg attagtcgaa gagtgcgtgc
    15541 gcggctatat caaaacccat taagtgttga taacaagccg ggtcagtatc gcaagatgca
    15601 ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa atgacatgaa gagataataa
    15661 ctatggatgt catgagaagt gttctgggaa tggtggtatt gctggcaatt gcgtttttgc
    15721 tgtcagtaaa caagaagagg atcagcctgc gtaccgttgg cgcggcgtta gtgttacagg
    15781 tcgtgattgg cggcattatg ctttggttac cgccagggcg ttgggtcgca gaaaaagtcg
    15841 cttttggcgt gcataaagtg atggcgtaca gcgatgcagg tagcgcattt atctttggtt
    15901 ctctggtcgg gccgaaaatg gacacgctgt ttgatggcgc aggatttatc tttggtttca
    15961 gagtattacc ggcaattatc ttcgtcaccg cgctggtgag tattctctac tacatcggtg
    16021 tgatggggat cttaattcgc attctcggcg gtatattcca gaaagcattg aatatcagca
    16081 agattgagtc attcgtcgcg gtcactacca ttttcctcgg gcaaaacgaa attccggcaa
    16141 tcgtcaaacc ctttatcgat cggctgaatc gcaatgaatt atttacagcg atttgtagtg
    16201 gcatggcctc gattgctggt tcgacaatga ttggttacgc cgcactgggc gtacctgtgg
    16261 aatatttgct ggcggcatcg ttaatggcga tcccaggcgg gatcttgttt gcccgcctgt
    16321 taagcccggc tacggaatct tcgcaggttt cctttaataa cctctctttc accgaaacac
    16381 cgccaaaaag cattattgaa gccgccgcta caggggcaat gactggactg aaaatcgccg
    16441 caggtgtggc gacagtggtg atggcatttg ttgcaataat tgcgttgact aacggtatta
    16501 tcggcggcgt tggcggctgg tttggttttg aacatgcctc gctggagtcc attttaggtt
    16561 acctgttggc cccactggcg tgggtgatgg gggttgactg gagtgatgcg aatcttgccg
    16621 ggagtttgat tggacagaaa ctggcgatta atgaatttgt cgcttacctc aatttctcac
    16681 cttatctgca aacagctggc actctggatg ctaaaaccgt ggcgattatt tctttcgcgt
    16741 tgtgcggttt cgctaacttt ggttctatcg gggtggtggt tggcgcattt tctgcggttg
    16801 cgccacaccg tgcaccggaa atcgcccagc ttggtttacg cgcgctggcg gcggcgacac
    16861 tttctaacct gatgagtgcc accattgccg ggttctttat tggtttagcg tagtgtgcga
    16921 tttatgagcc ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccatcgg
    16981 cactaatgaa agtgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg
    17041 tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca cggaaattgt ttccctccag
    17101 aaagctatat atcaggcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt
    17161 atgcgctggc gctgtcagca aaaattgctc tccatccttt tcgctgcaat aaactgactc
    17221 atcgggcaaa taaacaaaca gttgctgaac gccttgctga tgcaacgcat tcactgcggc
    17281 attacggtca gcatcgctgg tgatcgcctg tccccataaa atttccagct ccggtaaagt
    17341 gggtttcagg gtgtgaatat gcgccagcca gtgtttgatt ttgcccgctt tgaattctga
    17401 aacggtatcg acaaacaccg ggatttcatc agcgagggta aagacccact ccagcgcctc
    17461 ggctgtcagg ttgcaatcag ccagtaccac gcccgcatga cgaagtaaat cgcgcgaccc
    17521 gttcagtagt tgcggtgtca actgatccag cagatgggta tcgttaatcg ccagcacggt
    17581 ttgatcgtct cgattggcaa ttgccagata cgtcgatgtg ctttgaccat gtaaacgaac
    17641 gcagccggag acattcacgc cggcgcggcg cgtttcttcc aggagcattt cgccataaaa
    17701 gtcatcgcca atcactgaaa gcaaatggac gtcgcggcct aacagcgcca gattgtgggc
    17761 gatattgcgt ccaacgccgc ctgccgagca atgaatggta ccgggatgag aagccgcttg
    17821 cgggtaacgg atatccgcca tcccgcgaat atccatattg attgtcccca ccactacgca
    17881 gtattcctgc tcggtgagaa tgtaaccttt gcctttaatc cgacctttgc gcattaaatc
    17941 cataatatgc gccgcaacac gcgaacggct gatttgcagc atgtccgcaa tttcgttctg
    18001 ctgaatcagc gggttacgcc gtaaaattgc aaggatctcc ttctcccggt tgttcatctt
    18061 caggctaccg ctttttcagt ttgttgcgct ttcagccagg cgatctcttc tgcccagata
    18121 tccgggttga tggtttcaag gatcagcgga atgccgtcga aacggtcgtc ctgcatgatc
    18181 cagcggaacg catcatgacc gatattgcct tcaccgaggc tatgatggcg gtcaacgcgg
    18241 ctgccaaagg tgcttttcgc atcgttaagg tgcatcccgc gcagatactt aaagccgaca
    18301 atacgggcaa aatccgcgaa cgttttctcg cattcggctg gagtacgcaa atcatacccg
    18361 gcagcaaaag catggcaggt atcaatgcag acgccgacgc gggatttatc ttccacgccg
    18421 tcgataatcg ccgcgagatg ttcgaattta aaccctaagt tactgccctg accggcggtg
    18481 ttttcaatca ccgctgtcac accttgagtt ttatccagcg caatgttgat ggattcggca
    18541 atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg gtggaagttg
    18601 agcaaagaaa gccccagctg ttcgcaacgc tgcatttcat ctataaacgc atcgcgcgat
    18661 ttttccagag cttcagcgac cggatgtccg aggttaatca gataactgtc gtggggaaga
    18721 atttgcgccg atgtgtagtg atatttttca caggcggctt tgaattcatc gatggtttgc
    18781 gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc aaacgcggtt
    18841 gcgtcgattt cggcggcgcg aattgcggca tttgccagac cgccagcagc actaacgtgc
    18901 gctccaatgt atttcatgcg aggactcctg ttaaacccgc tggaggaaaa cggtaatgat
    18961 agcgggttaa caggagcaag atgtagtggt ttatgcgatg acgctttgaa tcacatagtt
    19021 aatcgcacca ccaccaacaa tcagccaggc aaacagtacc agtgccatca gcagaggttt
    19081 cgccccggct tttttcagtg cgctgacgtg agtggtcaga cccagcgccg ccatcgccat
    19141 tgccagcagg aaggtatcca gcgttaccag catgttcacc acgctctgcg gtaacaggtg
    19201 gaacgagtta aagatggcaa ctacgatgaa caagatggca aaccacggaa tagtgatttt
    19261 gcttttctcg ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca gcaggatgag
    19321 gaacggagcc agcatcatca cgcgcagcat tttggaaata actgctgcgt tttctgcatc
    19381 cgggctgatg gcatgacctg ccgccaccac ctgcgccact tcgtgcacag tagaaccaat
    19441 gtagataccg aaagtttccg gactaaacca ttgagacatc agcggatata tcgccgggta
    19501 gaggaaaatc gcgacggtcc cgaagataac aacggttgca acagccacgg ttactttact
    19561 ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac agatactgct
    19621 accggcaccg atcaaccagc tggtgtgctt atccagacca aacactttct gccccaggaa
    19681 gcaagccagc aggaaggtac tggacagcgt caacacgtca atgatgatcc cactgatacc
    19741 gacatcggca atttgcgaga acgtcagacg gaagccataa agaatgatac ccagacgtaa
    19801 taaatattgc ttggcaaaca gcacgccacc gtcacagctt ttccagatgt gcggatagat
    19861 ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg agggcactaa acccggcacc
    19921 cgcaaccgcg ggaatggaac caccccacag ggcgaccccg gtgataactg cactcagggc
    19981 taaccccgga ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga tattcgtcat
    20041 aaccctctcc tttaaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt
    20101 atatatttat aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt
    20161 tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg
    20221 cgttgtcgca atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc
    20281 aactgtttga tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc
    20341 cgcgcgcgtt ggcactgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca
    20401 acggcgcgat tcgtatctat gccagtagta ctatcggtaa ctacattctg cctgcagtca
    20461 ttgcccgtta tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc
    20521 aggacgtgat gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt
    20581 gccacagcac tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg
    20641 cgccgacttc gccattggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt
    20701 ggatcctgcg tgaacgcggc tccggcacgc gggagattgt cgattatctg ttgctgtcac
    20761 atttaccgaa gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg
    20821 tgcgtcatgg gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg
    20881 caggcacatt aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta
    20941 tacatcatcg gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc
    21001 ccgcgaatgt gccgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt
    21061 gacgcagcgc cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc
    21121 ctgggcgatc atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt
    21181 ctgctacaat cgcgcctcat ttttaagatg gatagcattt ttgtatgggt tccgaaacta
    21241 aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg
    21301 ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg
    21361 caggtccggg cggggcattg ctctcgtata tgttgattgg cctgatggtt tacttcctga
    21421 tgaccagtct cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg
    21481 gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact
    21541 gggcggtgac tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc
    21601 cggatacacc gggctggatc tggagtgcgc tgttcctcgg cgttatcttc ctgctgaact
    21661 acatctcagt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga
    21721 cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc
    21781 agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg
    21841 cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta
    21901 ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt
    21961 tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt
    22021 acaccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt ccgttcaccc
    22081 tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga
    22141 cggcggtgct gtcagcgggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc
    22201 tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc
    22261 gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg
    22321 gcaaccagac ggtatacctg tggctgctga acacctccgg gatgaccggt tttatcgcct
    22381 ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg
    22441 acattaacga tctgccgtac cgttcaggtt tcttcccact gggtccgatc ttcgcattca
    22501 ttctgtgtct gattatcact ttgggccaga actacgaagc attcctgaaa gataccattg
    22561 actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg
    22621 gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga
    22681 acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt
    22741 caattccatt tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg
    22801 ttatcgtttg cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc
    22861 cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa
    22921 ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc tcatggagat
    22981 atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt
    23041 gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt
    23101 ccgttgaaca aaaccttaaa gatgcacctg ccagtatcag cgtcattacc caggaagacc
    23161 tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac
    23221 tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca
    23281 ccctgattct cgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg
    23341 atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gtccgtggcc
    23401 cgatgtcgtc gctgtacggt tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa
    23461 aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg
    23521 atcgcggtga tacctataac ggtcagttct ttaccagcgg accattaatt gacggtgtgc
    23581 tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa
    23641 cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc gccggcaatg
    23701 tggaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc
    23761 gtcaggatcg tgattccgac tcgctggaca aaaaccgcct ggaacgccag aactactccg
    23821 tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatactac ggtgagaaag
    23881 tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat acggtcgacg
    23941 gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc ggtgaatggc
    24001 gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagttcc aaaacgtctg
    24061 ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg ctggcgctga
    24121 cgaccggcgt acgtatggac gatcatgaaa cctacggtga acactggagt ccgcgtgcct
    24181 acctggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg acggcattta
    24241 aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc cgtggcgcat
    24301 gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg gaactggggc
    24361 tttactacat gggcgaagac ggctggctgg aaggggtaga gtccagcgtt accgttttcc
    24421 gtaacgatgt gaaagatcgc atcagcatca gccgtacgtc tgacgtcaat gctgcaccgg
    24481 gctaccaaaa ctttgttggt tttacagcga acggtgtgcc ggtatttagc tactacaacg
    24541 ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat
    24601 ggaaactgtc gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca
    24661 aaccgctatc cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg
    24721 cgctggaaga ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgagccgaca
    24781 gcgcgacggc taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg
    24841 tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc
    24901 gtgacgacta cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct
    24961 tctgatgaga agatgcccgg cgaaccgggc ggactttcac ttcagtaaat attgcgcatg
    25021 gaagcgcaaa tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg
    25081 ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca gcactgcggg
    25141 ttgcagctgg tcggcaagaa actgatcatt atcaccctga tcgataagcg tcgggatcgc
    25201 atcctgcgcg ttactggcat acatcagcgc gcaactgtcc cattccagcc atgcattttt
    25261 gtcctcacct aaataggtgc taaacgcttt gattccccac gggacgctgc acggattcac
    25321 aattggcgca aaggccgaaa cgctagtgta tttacccgga tttttcagcg ccataattag
    25381 cgcaccgtga ccacccattg aatgaccgct aatagcgcag cggtcgctga cattaaattg
    25441 cgactgaatc agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc
    25501 ccaaggcggt tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc
    25561 gtcgttggca acctgttcgc cgcgcgggct ggtgtctggc attaccagta caatccccag
    25621 ttctgccgcc acgcgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa
    25681 tccggaaagc cagtacagca ctggaggcgg ggtgtgatca cgaggtggtg ggagaaagat
    25741 actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc
    25801 ttcaaaacag cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca
    25861 atttcttcat cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca
    25921 taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg
    25981 aatactggat tatgtgcgcc gcctcacgca caataatcag gctgtaaatc agcttaataa
    26041 ctttgccccc acgcagggcg gaggcgtcac acctgcagga gaaatcataa atgccatcac
    26101 tcagtaaaga agcggccctg gttcatgaag cgttagttgc gcgaggactg gaaacaccgc
    26161 tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata
    26221 tgaccgaaat catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc
    26281 cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaatt
    26341 tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg
    26401 atatcaccct gaccagcacc tgtgaacacc attttgttac cattgatggc aaagcgacgg
    26461 tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt attgtgcagt
    26521 tctttgccca gcgtccgcag gtgcaggaac gtctgaccca gcaaattctc attgcgctac
    26581 aaacgctgct gggcaccaat aacgtggctg tctctatcga cgcggtgcat tactgcgtga
    26641 aagcgcgtgg catccgcgac gcaaccagtg ccacaacaac gacctctctt ggtggattgt
    26701 tcaaatccag tcagaatacg cgccacgaat ttctgcgcgc tgtgcgtcat cacaactgat
    26761 taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt ttgttcgcgg cgtcgccatt
    26821 ctggggatcc tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat
    26881 cccgcctggt acggcgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc
    26941 atcggccagg tgaaattcct cacgcttttt gcgctgctgt ttggtgctgg cctgcaaatg
    27001 ttgctgcccc gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttgctgggc
    27061 tttattcacg gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggc
    27121 ttaatctgct ggcggctggt gcgcgatgcg ccatcagtaa aaagcctgtt taatacaggc
    27181 gtcatgcttt atctggtggg gcttggcgtt ttgatgttat tggggctgat ttccgacagc
    27241 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    27301 cttcacggcg gcgttgaagc gatcagtaat cgtgccgacg gtgttggcaa cagtttactg
    27361 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    27421 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    27481 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    27541 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    27601 tttcaggcga ttggctatgc gtcgctgttt tatggtttct ggccgcaatt aagccgcttt
    27661 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    27721 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    27781 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    27841 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    27901 gccggaccgg caatatctaa aacatcaaga taacgatctg gatcacattc gttaacaaaa
    27961 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    28021 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    28081 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgagtgctc
    28141 aataacagta cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgttagtgag
    28201 ctggattatc gaccaaatgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    28261 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    28321 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    28381 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcatt gattgttcac
    28441 tcaaaagcat tgagtgacga tgaactggca caatttatgg ataacattcc cggtatggtg
    28501 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    28561 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    28621 tcttccagtc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    28681 aaagagcagg atattattcc gccggaaagc tggattggca ctggcacgcc ggacatgccg
    28741 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    28801 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    28861 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    28921 ccgcaattaa cgactgtgcg ttatcccatt gcttcaatgg cgaaattagc caccgaactg
    28981 gccttgcagg gggcagcagg caatattgat ccgcgtgcca gccactgttt tatgccgacg
    29041 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    29101 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    29161 tagctatgat tgcaccgttt taacgttgta acccgtatgt aacagtgaat aatcactttc
    29221 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    29281 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    29341 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    29401 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    29461 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    29521 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    29581 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    29641 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    29701 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    29761 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    29821 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    29881 cagattcagt tcgtactgct gaaaggtgag ccgggccatc cggatgcaga agcacgtacc
    29941 acttacgtga ttaaagaatt gaacgacaaa ggcatcaaaa ctgaacagtt acagttagat
    30001 accgctatgt gggataccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    30061 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    30121 gaagctctga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    30181 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca ccgtactgaa cgatgctaac
    30241 aaccaggcta aagcgacctt tgatttggcg aaaaacctgg ccgatggcaa aggtgcggct
    30301 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    30361 aaagacaacc tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    30421 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    30481 ggggaatact tgttggaaat gagcggtatc aacaagtctt ttcctggtgt taaggcactt
    30541 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggc
    30601 gcaggaaaat caacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    30661 attttattcc agggtaaaga gatcgatttc cattctgcca aagaagcgct ggaaaatggt
    30721 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    30781 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    30841 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    30901 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    30961 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    31021 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc acataaaatg
    31081 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    31141 actgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    31201 cttaaccagc gtttccctga caaagaaaac aagccgggcg aagtgattct tgaagtgcgt
    31261 aacctgacgt cgctgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    31321 gagatcctcg gtattgccgg tctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    31381 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaaa gatcaataac
    31441 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgaaga gcgccgctca
    31501 acgggaattt atgcctatct ggatatcggt tttaactcgt taatttccaa tattcgcaac
    31561 tacaaaaata aagttggttt actggataac tcgcggatga aaagtgatac ccagtgggtg
    31621 attgattcga tgcgggtaaa aacaccgggg catcggacgc aaattggttc gctctccggt
    31681 ggtaatcagc aaaaggtgat tattggtcgc tggctgttaa cgcaaccaga aatattaatg
    31741 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgagatcta tcagttaatt
    31801 gccgagctgg cgaagaaagg caaggggatt attattatct cctccgaaat gccagagttg
    31861 ttagggataa cagaccgtat tctggtcatg agtaatggtc tcgtttccgg aattgtcgat
    31921 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    31981 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    32041 atttacgtcg ttcttttagt tttgctggca attattattt tccaggatcc tacattttta
    32101 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctt
    32161 ggtgtggcag ggttaattgt cacccagggg accgaccttt ctgcggggcg tcaagtaggg
    32221 ctggcggcag tggtggcagc gacattattg cagtccatgg ataacgccaa caaagtgttc
    32281 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    32341 gtgatcggtt tgatcaacgg tctgattatc gcctatctca acgtgacgcc gttcattacc
    32401 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    32461 gcgtcgccga tttctggttt cgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    32521 ctggggagtt tccgtctctc ttacatcacc ttctacgcgc tgattgcggt ggcgttcgtc
    32581 tgggtgttgt ggaacaaaac ccgcttcggc aagaacattt ttgctatcgg cggtaacccg
    32641 gaagcggcaa aagtttctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    32701 tctggtgtgt tctatgcctt cggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    32761 aacaacctcg gctttatgta tgaactggat gctatcgcgg cgtgcgtggt gggcggcgta
    32821 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    32881 gtcatcaact atggcctgac gtacatcggt gtaaacccgt actggcagta catcatcaaa
    32941 ggggcgatta ttatcttcgc cgtggcgctg gattcactga aatacgcgcg taagaagtga
    33001 tgtgattgtg ggtattgccg gatgcgtcgc atccggcaat cggtgccaaa tgccggatgc
    33061 gtcgctggtt ttacaatgtt acagagtgtt ctttctctcc cttcttaaac ttcacttccc
    33121 caagctcaat acaacccacc gggcagacgt gaccacaaag cagacaaccc acgcatttct
    33181 cggtattaca atgcggcgtg cgtgttttct cgctccattc catcgcctgg tgaccgccgt
    33241 cgtaacagga aatataacag cgtccacagc caacacattt atcgagattg atacggggat
    33301 agacaatata actgcggtct aaatcttccg ccggaacaat attgttattc gccagaccta
    33361 ccatctcctg caacgaatca aatccctgat cggcgagata atgcgacaac ccgctcgcca
    33421 tatcttccac tatccgatac ccgtactgca taatgccggt ggtcacctgt aacgttgctg
    33481 cgccgagcaa taaaaactcc gccgcatcct cccaggtttc aatgccgccg ataccgctga
    33541 tcgggaaatc gcgcagctct ggatgggtac gtatttgctg aatgaagcgc agggcgatcg
    33601 gttttaccgc tttaccggaa tatccggaaa tactcgattt accgttaacg atcggcatac
    33661 cgattttctg attaagatcg atattggtga tggatttaac ggtgttaatc gccgcaatgc
    33721 catctgcgcc gccgcgcttc gccgccagcg ccacttcgca catatcaccg atattcggcg
    33781 tcatcttcgc cagcattggc agcgtggaac cacgttttac tgcccgacaa tatttttcta
    33841 ccagctccgg gctttgcccg acatcgctac ccatcgcatg agaagtcatt tgcggacagg
    33901 agaagttaca ctcgatcata tccgcgccag cttcttgcac cagacgcgcc agctcttccc
    33961 attgctgctc attttcgccc atgattgaag cgatcaatac tttgtccggg taatcttctt
    34021 tcagccgacg cagggcggcc agattatctt ccaacggatg ttcagcaatc tgctccatat
    34081 ttttgaagcc gataaaaccg gtatcttctt tcaccagatg atcaaaacgc ggcgagactt
    34141 cgttggcgat aaaaaagccg atcgttttaa acaccacgcc gccccagcca gtgtcgtagg
    34201 ctttggcaca catctcatag cagttgccta ccggcgaaga agagaggcag aacgggttgg
    34261 gaaacttcac gccgcaaaaa gtaatcgaaa gatctttcgt taacatgagc aagctccctc
    34321 taaatagtga tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt
    34381 tttgtccccc tcaacaatat cgccagcagc aaagacttgc ggatcgcggg tctggtaatt
    34441 ttgtgtattg atggtgttac gctgcggctc taactccgca aaggcatcca gtctggcatg
    34501 ctgaccgacg gcgagaataa ttttatctgc cgccatcgtc agttcgcccg gtaaccgtac
    34561 atgcttaaac gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga
    34621 aacgcccagc tcccgagcgc tggcaaactc tttttcgctt gccggaaact catctaactc
    34681 ttcacgcgct acgcaagtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc
    34741 catcgcgaca tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg
    34801 cgcctgtcgt gcacgttgca agaagtcgac ggcaatctca acgtcgctat gctcaaacag
    34861 tggtagcccc gaaccgctcg ataaccccac ggtgaccagt accgcgcggt tttccgcttt
    34921 cagctgatcc agggtaaatg tgttaccgat ttcgttattg cacttaatgg tcacgcccat
    34981 tttttcaata cgggcgattt ctgcatccag tactgattgc ggtaaccgga attgcggaat
    35041 gccgttacgc aaccagccac cgggttgtgc ttctttctca tagatcgtga cgtcataacc
    35101 ctggtttgtc agcgtcacac tggcctgcaa tccggcagga cctgcgccaa taatggcgac
    35161 tttgccgagc gttttagtac cgggctgata aatttccatc ccagtttgtt gttcaaaatc
    35221 agtaacaaaa cgctgtaagc gaccgatatc aatgggagta tcaacaccgg cacgggtaca
    35281 accgctttgg cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt
    35341 attttcgcga attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa
    35401 tttccccgga tcggtctgtg ccgggcaagc ctgactacag ggggcgtcat ggcataataa
    35461 acagcgagag gcttctttaa tcgccagtaa cggtgtaaaa gccggagtga gttcatccag
    35521 ataattttgt tgcggcatgt gatcttcctt aatgctatga gtaattaagg cgaacgctaa
    35581 atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gggttattcg gcccatccat
    35641 gggcctcacc ccttatgggg ccgctgcagg cagcgttcaa atccgctcca tgcggatttg
    35701 tcgctcaccc cagtcactta cttatgtaag ctcctgggga ctc
//
LOCUS       DARNVM010000028        35616 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.028, whole genome shotgun sequence.
ACCESSION   DARNVM010000028 DARNVM010000000
VERSION     DARNVM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.028"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            67..1722
                     /gene="aslA"
                     /locus_tag="SI621_001315"
     CDS             67..1722
                     /gene="aslA"
                     /locus_tag="SI621_001315"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HEI4064052.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1824..3059)
                     /locus_tag="SI621_001316"
     CDS             complement(1824..3059)
                     /locus_tag="SI621_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEI4064053.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGAEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3206..3282)
                     /locus_tag="SI621_001317"
     tRNA            complement(3206..3282)
                     /locus_tag="SI621_001317"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3246..3248),aa:Pro,seq:tgg)
     gene            complement(3325..3411)
                     /locus_tag="SI621_001318"
     tRNA            complement(3325..3411)
                     /locus_tag="SI621_001318"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3375..3377),aa:Leu,seq:cag)
     gene            complement(3432..3507)
                     /locus_tag="SI621_001319"
     tRNA            complement(3432..3507)
                     /locus_tag="SI621_001319"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3472..3474),aa:His,seq:gtg)
     gene            complement(3566..3642)
                     /locus_tag="SI621_001320"
     tRNA            complement(3566..3642)
                     /locus_tag="SI621_001320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3606..3608),aa:Arg,seq:ccg)
     gene            complement(3745..5130)
                     /locus_tag="SI621_001321"
     CDS             complement(3745..5130)
                     /locus_tag="SI621_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEI4064054.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRNGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(5321..6061)
                     /gene="rffM"
                     /locus_tag="SI621_001322"
     CDS             complement(5321..6061)
                     /gene="rffM"
                     /locus_tag="SI621_001322"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HEI4064055.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQMLFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6064..7416)
                     /gene="wzyE"
                     /locus_tag="SI621_001323"
     CDS             complement(6064..7416)
                     /gene="wzyE"
                     /locus_tag="SI621_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HEI4064056.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIHGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7413..8492)
                     /gene="rffT"
                     /locus_tag="SI621_001324"
                     /gene_synonym="wecF"
     CDS             complement(7413..8492)
                     /gene="rffT"
                     /locus_tag="SI621_001324"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HEI4064057.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSDHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLALFSEENLQILNEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8489..9739)
                     /gene="wzxE"
                     /locus_tag="SI621_001325"
     CDS             complement(8489..9739)
                     /gene="wzxE"
                     /locus_tag="SI621_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HEI4064058.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9741..10871)
                     /gene="rffA"
                     /locus_tag="SI621_001326"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9741..10871)
                     /gene="rffA"
                     /locus_tag="SI621_001326"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEI4064059.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPVGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10876..11550)
                     /gene="rffC"
                     /locus_tag="SI621_001327"
                     /gene_synonym="wecD"
     CDS             complement(10876..11550)
                     /gene="rffC"
                     /locus_tag="SI621_001327"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HEI4064060.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGAIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11528..12409)
                     /gene="rfbA"
                     /locus_tag="SI621_001328"
     CDS             complement(11528..12409)
                     /gene="rfbA"
                     /locus_tag="SI621_001328"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEI4064061.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKLVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12428..13495)
                     /gene="rffG"
                     /locus_tag="SI621_001329"
     CDS             complement(12428..13495)
                     /gene="rffG"
                     /locus_tag="SI621_001329"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEI4064062.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPACVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYHDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13495..14757)
                     /gene="wecC"
                     /locus_tag="SI621_001330"
     CDS             complement(13495..14757)
                     /gene="wecC"
                     /locus_tag="SI621_001330"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HEI4064063.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSDRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14754..15884)
                     /gene="wecB"
                     /locus_tag="SI621_001331"
     CDS             complement(14754..15884)
                     /gene="wecB"
                     /locus_tag="SI621_001331"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEI4064064.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALANIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15940..16986)
                     /gene="wzzE"
                     /locus_tag="SI621_001332"
     CDS             complement(15940..16986)
                     /gene="wzzE"
                     /locus_tag="SI621_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HEI4064065.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16998..18101)
                     /gene="wecA"
                     /locus_tag="SI621_001333"
     CDS             complement(16998..18101)
                     /gene="wecA"
                     /locus_tag="SI621_001333"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HEI4064066.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18329..19588)
                     /gene="rho"
                     /locus_tag="SI621_001334"
     CDS             complement(18329..19588)
                     /gene="rho"
                     /locus_tag="SI621_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEI4064067.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19673..19774)
                     /gene="rhoL"
                     /locus_tag="SI621_001335"
     CDS             complement(19673..19774)
                     /gene="rhoL"
                     /locus_tag="SI621_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HEI4064068.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19915..20244)
                     /gene="trxA"
                     /locus_tag="SI621_001336"
     CDS             complement(19915..20244)
                     /gene="trxA"
                     /locus_tag="SI621_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEI4064069.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20375..21640
                     /gene="rhlB"
                     /locus_tag="SI621_001337"
     CDS             20375..21640
                     /gene="rhlB"
                     /locus_tag="SI621_001337"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HEI4064070.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21776..23260
                     /gene="gppA"
                     /locus_tag="SI621_001338"
     CDS             21776..23260
                     /gene="gppA"
                     /locus_tag="SI621_001338"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HEI4064071.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23307..25328)
                     /gene="rep"
                     /locus_tag="SI621_001339"
     CDS             complement(23307..25328)
                     /gene="rep"
                     /locus_tag="SI621_001339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEI4064072.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25546..25995
                     /locus_tag="SI621_001340"
     CDS             25546..25995
                     /locus_tag="SI621_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEI4064073.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26147..26428
                     /gene="ppiC"
                     /locus_tag="SI621_001341"
     CDS             26147..26428
                     /gene="ppiC"
                     /locus_tag="SI621_001341"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HEI4064074.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26474..27949)
                     /gene="ilvC"
                     /locus_tag="SI621_001342"
     CDS             complement(26474..27949)
                     /gene="ilvC"
                     /locus_tag="SI621_001342"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEI4064075.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28099..28989
                     /gene="ilvY"
                     /locus_tag="SI621_001343"
     CDS             28099..28989
                     /gene="ilvY"
                     /locus_tag="SI621_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HEI4064076.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28986..30530)
                     /gene="ilvA"
                     /locus_tag="SI621_001344"
     CDS             complement(28986..30530)
                     /gene="ilvA"
                     /locus_tag="SI621_001344"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEI4064077.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30533..32383)
                     /gene="ilvD"
                     /locus_tag="SI621_001345"
     CDS             complement(30533..32383)
                     /gene="ilvD"
                     /locus_tag="SI621_001345"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEI4064078.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELV
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32448..33377)
                     /gene="ilvE"
                     /locus_tag="SI621_001346"
     CDS             complement(32448..33377)
                     /gene="ilvE"
                     /locus_tag="SI621_001346"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEI4064079.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33397..33660)
                     /gene="ilvM"
                     /locus_tag="SI621_001347"
     CDS             complement(33397..33660)
                     /gene="ilvM"
                     /locus_tag="SI621_001347"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HEI4064080.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33657..35303)
                     /locus_tag="SI621_001348"
     CDS             complement(33657..35303)
                     /locus_tag="SI621_001348"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HEI4064081.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPVTCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQVHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35306..35356)
                     /locus_tag="SI621_001349"
     CDS             complement(35306..35356)
                     /locus_tag="SI621_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064082.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(35443..35541)
                     /gene="ilvL"
                     /locus_tag="SI621_001350"
     CDS             complement(35443..35541)
                     /gene="ilvL"
                     /locus_tag="SI621_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HEI4064083.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8642 a         9797 c         9032 g         8145 t
ORIGIN      
        1 agggatctca attatcaatg cttaattacg tcaacatttt gacgacatga aaccggagca
       61 ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg tggctgtcgc cgccgctctt
      121 ccactcatgg ccaacgcagc agataccccg tcaaccgcca ccgcacgcaa aggctttgcc
      181 ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc cgacaacatg
      241 atgccggtga tgcagcatcc tgcacaagac aaagaaaccc agcagaagct ggcagaactt
      301 gagaaaaaaa ccggtaagaa accgaacgtg gttgttttct tgctggatga tgtgggctgg
      361 atggatgttg gctttaacgg cggcggcgtg gcggtgggta atcctacacc agatatcgac
      421 gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccgag ttcttcccca
      481 acccgcgcca cgattcttac cggacaatac tccatccacc acggcattct gatgccgcca
      541 atgtacgggc aaccgggcgg tctgcaaggg ttaaccactc tgccgcagtt gctgcacgat
      601 cagggctacg tcactcaggc catcgggaaa tggcatatgg gggaaaacaa agagtcgcag
      661 ccgcagaacg ttggctttga tgatttccgc ggctttaact cggtatccga tatgtatacc
      721 gaatggcgtg atgttcacgt caacccggaa gtcgccctga gcccggatcg ttctgaatac
      781 atccaaaaac tgccgttcag caaagatgac gttcatgccg tgcgcggcgg tgaacaggaa
      841 gccattgccg acattacgcc gaaatatatg gaagacctgg atcaacgctg gatggaatat
      901 ggcgttaagt tcctcgacaa gatggcgaag agcgataagc ctttcttcct ctactacggc
      961 actcgcggct gtcacttcga taactaccca aatgcgaaat atgcgggtag ctctccggcg
     1021 cgcacctctt acggcgactg catggtggag atgaacgata tcttcgccaa tctgtataaa
     1081 gcactggaga aaaacggtca gcttgataac acgctgattg tgttcacctc tgataacggt
     1141 ccggaagccg aagttccgcc acacggtcgc acgccgttcc gcggtgcgaa aggttctaca
     1201 tgggaaggcg gcgttcgtgt gccaaccttc gtttactgga aaggcatgat ccaaccgcgt
     1261 aaatctgacg gcattgtcga tctggcggat ctcttcccta cggcgctgga tctggcgggg
     1321 catcctgggg cgaaagtagc gaatttagta ccgaaaacca cctttatcga tggtgtggac
     1381 cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga gcactacttc
     1441 ctcaacggta aactttctgc tgtgcgtatg gatgagttca agtatcacgt cctgatccag
     1501 caaccgtacg cgtataccca gagcgggtat cagggtggat tcaccggcac agtaatgcaa
     1561 acggcgggat cgtcggtgtt taacctgtac accgatccgc aggaaagcga ctccatcggc
     1621 gtgcgccata tcccaatggg tgtaccgcta cagaccgaaa tgcacgcgta tatggagatc
     1681 ctgaaaaaat atccaccacg cgcgcagatt aaatctgatt aagccggcgc tatcgctaca
     1741 tcgggccaca ctgaccttgc cggatgcgac gtgaacgcct tatccggcaa ttaaccgcag
     1801 gcggccacaa aatggccgct tttttactta ttcaccacca tcaaatgcgc ctgcataatg
     1861 tcgctggccg gacgaccgtg cgccagcaaa tcagccattg ctttaagata tggcggtaga
     1921 tggcggaaat aacgctgata cccggcacac aaataattca gcccgggttt gccgctggca
     1981 tcgagcataa agcggtgttt cggacagcct ccccagcacg cttttaacac gttgcaactg
     2041 cgacactgcg ccggtaactg cttaaattta tcttcaccaa acacctgctg ttgcggggaa
     2101 tctaccattt ctgcaattgt ttgctggtgc atattcccca gtcgatattg cggataaaca
     2161 tagtgatcgc aggcgtaaac gtcgccgttg tgttcaacaa tcaccgagcg cccacaggtt
     2221 ggctgatgat ggcaaaccgc acctggcgca ccaacaaaat tggcaaacgc ccattcgata
     2281 ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaaa
     2341 aattcaccga actcatcggg gcgtaccgac cattccgtca gttcgccctg aatatcgccg
     2401 ggagcatgta gcttaagtcc agcatgagca gccgtttcat cagccaggcg ctcgaccacc
     2461 ggaataaact ggatgaactc agcgcctgca tcacacagaa aatcatagac ttgcaaaggt
     2521 tgtaacgcac tggtgcgatt aacgcagacc agcacgttat agtcgacatg atgtttttgc
     2581 aggagcgtca gggcacgcat caccagctta tgcgttggtc tgccgccttt ggtcacgcga
     2641 tattgattgt ggatctcagc cggaccatcc agcgataacc ctacaagaaa atgattttct
     2701 gccagaaatg cacaccattc gtcatcaagc agaacgccgt tagtctggaa gctgttactt
     2761 atcttcctgc cagcaccata tttcgcctgt agcgccacgg cacggcggta aaaatccagc
     2821 ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg ggtttcgctg
     2881 gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg cgtgactggc
     2941 ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt cagattacaa
     3001 tcggaaccgc tcggtttcgc catcacatga aaagcacgcg ttggaacctg ttgcagcatg
     3061 gtcgctcctt gcctgaagtc atccttcaat aagttaaggg cgaaatcata ccgtggcgca
     3121 atgagttatt acgggcgttt attgcgaagg gaaagagacg aaatgcacag aaaacaaaaa
     3181 accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc tccgacccac
     3241 tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg ctttttgaat
     3301 ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt
     3361 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cattgtcaca
     3421 acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca
     3481 gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca
     3541 ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct
     3601 gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg
     3661 tggataatac ggacttccca cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg
     3721 gttacgattt gtgcgttttg ctgcttattc ctccagttta tgcgctttac cgtggcgatt
     3781 aagcccaaaa attttataaa tcgccgtcac cgccagcata aagatgatgc cgacaaacag
     3841 cgacatacgc gtatcttcat taaagtacat gccgatcaaa acgcaaatca ggaatgccat
     3901 tgttacgtag ttggcccacg ggaacaggat tgagcggaac ggatggctgg caatcgccgc
     3961 tttatgcgca cgacgaaaac gcagctggct tatcaatatc acaaaccatg gcaccatccc
     4021 cggaagcacg ctggcactgt agacgtagac aaacacacgc tgcggattgg gaatgatgta
     4081 gttcaggcat gagccaatta acaggatagc aatagatact gccacacccg caaccggtac
     4141 gccgttacgg gaaactttcg ccatcgccgt cggtaactga cggtttttcg ccagtgcgta
     4201 gagcatacgt ccgcaactgt acatgccgct gttacagcca gagagcgcag ccgtcagcac
     4261 cacaaagttg ataatgcccg ccgctgcggt aatgccgatt ttggcgaaag tcagtacgaa
     4321 cgggctaccg ttgctgccta tttcattcca cgggaagata gtaacgataa cgaaaatcgc
     4381 gcctacgtag aaaatcagaa tcctccacag cactttgcct actgcactgc gcagcgtcac
     4441 ctgcggattc ttcgcttcac cagcggtaat gccaatcagc tccacgccct ggtaggacgc
     4501 caccacaata cacaatgcgg tcaggaaccc tttccagccc cccgcaaaga aaccgccatg
     4561 ctctgtgagg ttgctaaaac caatcgactg cccgccattg ccaaagccaa agaaaatcac
     4621 gcccaggcca atgacaatca tcacgataat cgtggtgact ttgatcatcg cgaaccagaa
     4681 ctcgatttcg ccgtacaacc gcaccgccgc cagattcgcc aacgccacca gcgccaccgc
     4741 gatcaatgcg ggtatccact gcgccatctc cgggaaccag aactggacat aaacgccaat
     4801 ggcggtgatt tctgagatcc ccaccgccat ccacataaac cagtaagacc aggcggtgag
     4861 atagccaaag aacgggctca tataacgatg cgcataaacg gcgaacgaac cggtaaccgg
     4921 ttcgaggaat aacatttcgc ccattgaacg catgatgaaa aagacgaaca gcccggcgat
     4981 gatataggcc aacaatacgg atggcccggc ccatttcagg gtactggcgg cccccataaa
     5041 caggccgacg ccaatggtgc cccccagggc gatgagttcg atatgtcgag cttccagccc
     5101 acgctgtagc tctggtttgt tatctgccat aaatcctcgt gttgtgtttg catgctttcc
     5161 ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg aatgttttcg taattcagga
     5221 gaaatggcaa ataaagcatt aaaaatttga atgctttgtg taataaaaaa gcaggaaggc
     5281 gacgaagtga ccactccgtc gctttacaga gagaggaaaa tcagaggttg ccggtgtaat
     5341 gccagcgtaa ataacgcagc aaacgaagct gacgcttaat gcggctcggc tgcgaaagca
     5401 ggcggtagag ccactccagc cccagcgttt gccagatttt cggtgcacgt tttacgtgac
     5461 cggtgaaaac atcataagtc ccgccaacgc ccatatacag cgcatctgga tgcaccagac
     5521 ggcagtcgcg catgaagatc tcctgctttg gcgatcccat cgcaacggtg acgatttgcg
     5581 ctccgctggc atgaatgcgt tcaaacagca tctgacgctg ctcgggttta aaataaccat
     5641 cctgactgcc aacgatattc acattccact ggttgcgtag tttagcttca gtttgcgcca
     5701 gcacttcagg tttaccgccc accagaaata ccggcgtccc ttctttgcct gcgcgcgcca
     5761 tcagctcttc ccagagatcg gccccggcaa cgcgggaaac ctgcgcctgc gggtactttt
     5821 tacgtactga acgtacaacg ctgatgccat ccgcatattt aaattcggca gcgttaatta
     5881 actccctgac ctcggcgtta tcttcaatgg tcagcatttt ttcagcatta atggcaacca
     5941 gcgttccctg cttaagctgc ccgtcagcga acagataatc gagggcgtgc tgcatatcac
     6001 gccaaccgat caactgtaag ccacgcagcg tataggtcgg tgccgtggtg ttgttattca
     6061 ttgttatcct tcaacctgcg tccggagcga tgattttgta cgtttatgaa tgagtccggc
     6121 gctttcaaaa agccagtaca gcagttttgc gatcatcaga catgcgccga agaccacgat
     6181 aaaaaagact acgcgtgaga caaacgaatc cagcccttca cgcgccagca cgatcatatt
     6241 gaagatcgcc ccaaagcaga aactgtgcaa tatcgcagct ttatagcgat taggctcgcg
     6301 gttgcccagc tcatacagcc agtcgaacca tttgatgatc agaccaacca cgatcgcccc
     6361 gagcgggatg aacaacgcgc cgcccatcac caccagtgag cctataagcg taggcgagat
     6421 cgccagcccg gagtggttat tcagcacttc ccaggtaaag tagttggcgg agttcagtac
     6481 catactcggg cgacccggcc acagccagga agggataaag acatagaaat cgcggacaat
     6541 gggagccaga ccctggaagt cgatgttgtc gtagttctgc aacagcaatg ccagattctc
     6601 ccacggcgaa aaggtatcgc gggtgagata gagaaacgta tagaacgctt catcaccgct
     6661 cacattcatt ccatagcgtt ttagcgccag ccagaacatg ccaacaatcc ccagcacgcc
     6721 cgctgccgcc agcatccaca acgaaatcca gccgtgaata atgccaataa acaggaagat
     6781 agcgaatgcg ataatgatat tggcgcgagt gccgccgaca atcatataag tcagcaagcc
     6841 aaaagcgacc gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta
     6901 gaccaccagc atcgccggga tgaaaaagta aaagaagcgt tttaacgcca cgccggagac
     6961 ttcactggag aagatctgac tgtaggagtt aagccggaac agcaaaaatc cgttgtgcat
     7021 gaagaagatg ccgacgctta ccagcgcgat gcccatcagg atcacccacg tgagattggt
     7081 ctccacgcgg ttcatggtaa acagcggacg gcgcggtaca tcggcaacgc gtttgcgtag
     7141 gcgggttttg taggtgacgt agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg
     7201 caacaagatt tctggcggcg cgacaccaac atcaaagcga aataccagca cgctggtcag
     7261 cgggaagccg aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa
     7321 acgcacacgg cgaaactcaa accaggtcag cgtggcaata aacagcgtgc agagcagcca
     7381 gacaacaaac aagccactga attgcagcag actcatgcga cctccccggc ggcaatcgcc
     7441 aacgcccgct gccagccttg cagatagttc gggctaaaga aggcaatggt gtttttatcc
     7501 accgacgcca actggcgctg cgcttcacgc acaatatcct cgttgagatc gtcggtagta
     7561 aacagcaccg gcaaatgctg ttccgtcata tcctgccaga acggattttc ccggttaagc
     7621 acacaaggaa tgcccgcctg aatcagtaag cacagcgtac caatgccctg ctggcgggca
     7681 aaaataaagt aaccaagatc gcactgacga agtagcgcca gataggcgtc aaattccagt
     7741 ttttcgttca gaatttgcag attttcttcg ctgaataacg ctagccccgc ctgacgaact
     7801 tcctcgatgt acgcttcgtt attaggcgga tatcccatcg gcaccaccac ttttaccgta
     7861 tcgccaaatt gctgatgaac ggcgcgcaag gcagcaatat gctcattgct gcggtcgccg
     7921 gagttgccca ccagaatggt cattttccct tcacgttgcc gatcgttcgc catcgtattg
     7981 agcgaaggat ccatccgcgt cgggaagtac agcagttcgc cccgcacctt tgggtgcgtt
     8041 ttggcaaaaa agctcaaatc accgcgggtg gcaaatacac agccgactcg cttttgcgcc
     8101 aggcgacgta gtgggtaaaa aagcttatat ctcaagccac tggaaagctc atacaggtct
     8161 gccccccaga tatgccagaa aaactggctg ggcttaatgc caccactcag cagagccagc
     8221 cacagtgtgg gattgaactg accgtggaaa aaaaatcgct gctgacggtt agcttttgct
     8281 ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga aaaattgcac agaaagcgcc
     8341 ggacagctat cacttaagcc gtcgtccttg ccaacaacca taaactcgcg cgcgtgatcg
     8401 ctcgtcgcgg ccagcgcgtc gttgaaaaac cgcaaaacgg ttcggttatg gtgagggata
     8461 tccgatccca gtacgtgaat cagtacagtc atgcccgcct acgccagagt aaaaacacgc
     8521 cacaacaaag agaaaaatag acgatatagg ttgccatata ggcctgcgcc gcgcccagtg
     8581 caccatgcgc agggattagc cagtgggcaa ataccatcaa taaagtgaac tggctgactt
     8641 ccgccagaat ataaaaccgc agtgacgctt tggcgatcac cagataacca aagacataag
     8701 cgcccacttt taacacatca cccaccaact gccaggcaaa gagatcgcgc atagcggtaa
     8761 atttattcga caacagcagc cagatagcaa aatcacgcag caaccagacg gtaaaactcg
     8821 ccgccgccac tgccggtaag acgaatttta gcgatttaac cacttcccgg gtgatatcgc
     8881 gcttttccgt tagccgcgac aacgtgggca gcaaatatac gctgaacgat gccgtaataa
     8941 attgcaggta ggcatcggaa atactgctca ccccttgcca gatccccacc tcatcccagc
     9001 tatactgcgc cgccagcagt ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg
     9061 taatcaacgc catgagcgta aatttgctca actgccctgc cagaccgtta tcccagctgg
     9121 gtttcagata gcttaacggg atgacaccac gtttgatcaa catgatggcg gcaggaatta
     9181 ccaccagcgc gggaatcagc gccagaccca gcaacgcccc ttcataaccg cccaaacggt
     9241 aactgacgta gtacgcgaga acgccaatca ggctgccgac aatcagggat aacgcattac
     9301 ctgctgcatc gcgaaagcct ttcatcaacg ccagtaacag gttgccccag gcaatcccca
     9361 tttgcaccag cgccactaaa cgcaccagtc cctgatagtc ggtattacca aacaatccct
     9421 ggctgattgg cgctgctgcc agcacaaaaa ccagcgccat tagcgtagag aagccaagta
     9481 ccatcgctga tgaagtgccg accacgcggc gcagctgttg gggattatca tggtactgag
     9541 caacgtattt ggttacgcca ttaaagatgc cagccccggc aagcacgccg agcacggtaa
     9601 tcaactggcg gaaatttgcc gccagcccaa gccccgccgg accaaatgac accgccagca
     9661 acttaccgac cagtaacccg gcaccaatct tgaccagtgt actggccgcc gtccacaagg
     9721 acgcttttgc caacgacata tcaggagaag tagttcaaca aagtcgcaat taccgtacgc
     9781 tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc actctctttg
     9841 gtggtgtagc gatcttcacc gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc
     9901 agcggaatgt aatggaacac cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg
     9961 ctccggtcat caatatcccg cagtttaatg tagaacatat gcgcgttctg cacgcagcca
    10021 acgggaatcg acggcagctc gatacgcccg gctttcgcca gaggtgctaa cgcatcgtag
    10081 tagttttgcc acagcgccag acgttgctga ttgatacgat ctgctgcttc cagttgcgcc
    10141 cacaggtacg cagcttgcag atcggacatc aaatagctgg agccgatatc gcgccaggta
    10201 tatttatcga cctgaccacg gaagaactgg ctgcggtttg tgcctttttc acggatgatc
    10261 tcggctcgtt cgatcaacgc tttatcgtta atcagcgtcg cgccgccttc accgcccgcc
    10321 gtgtagtttt tggtttcatg gaagctaaag cagccaatat gaccaatggt tcccagtgca
    10381 cgccctttgt aagtggacat cacgccctga gcagcatctt ccaccacaaa cagattatgc
    10441 tttttcgcca acgccataat ggtgtccatt tcgcaggcca cacccgcgta atggaccggc
    10501 acgataacgc gcgttttgtc ggtgatcgct gcttcaatca acgtttcgtc gatgttcatg
    10561 gtgtccgggc gaacatccac aaaaacgatt tttgcgccac gcagcacaaa ggcattggca
    10621 gtggagacaa aggtgtagct cggcatgatc acttcatcgc caggctggat atcgagcagc
    10681 agcgccgcca tctccagcga agcggtgcag gatggcgtca gtaacacttt ggcgctgcca
    10741 aaacgttgct ccagccactg ctggcagcga cgggtaaaac cgccatcgcc acacagtttg
    10801 ccactaccca ttgccgactg catatagtcg agttcggttc ccaccaccgg cggtgcgtta
    10861 aatggaatca tgtgatcacc tgtataacca gtacgcggtg ctttctacat tcgcaccact
    10921 ttgtatgtat cgttttagcg cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt
    10981 tttaccgcga gcatacgccc agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg
    11041 tccagccagc aggccaattc gcgcatctgt cgcattgagt tcccgtaaag agacatagcc
    11101 gcgaatagcg ccggacgccg cacgtaaaat cagacattga tgatcaaagg tgccgcgcac
    11161 ggcattttca atccactgtg cataaaagcg accgctggcg tcaggcgcat accacggcgc
    11221 acgaaaacgg ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg gaatatctgt
    11281 ctcttgtgcc acttctgcgc cgctatcacc gacattgttc acgggtagcg ccaaatcaac
    11341 ttcaccttct accagggaga atcccagctg ttgcagggca tccagttcac ccgtatttga
    11401 tgccgcaatt ttggcctgca cccgtgacca cggcgctaac gcgtctggcg tcaggagcgg
    11461 tgcttcagac gtgatgcgca cgatggcgct gttaacacca aagaaggcgt tttcccaggt
    11521 tagtggctca atactggcgc ggacgggcac gaagtaactc cagcagatat tggccgtagc
    11581 cagttttcgc cagcgagctg gcggcgcgct tcacgccttc gtcatcaagc cagccgttac
    11641 gccaggcgat ctcttccagg caggcaatct taaagccctg gcgtttttcc accgtctgta
    11701 caaaggtgct ggcttcaatc aggctgtcgt gagtgccagt atccagccag gcaaatccgc
    11761 gcccgagcag ttcaacggtc aggttgcccg cctcgaggta catctggttg atggaggtaa
    11821 tctccagttc accacgctcc gacggcttca ccagctttgc gtactccacg actttactgt
    11881 cgtagaaata aagcccggtc accgcccagt ttgacttcgg ctgttttggc ttttcttcca
    11941 gcgagatagc gcggaaattg tcgtcaaact ccacgacgcc aaagcgttcc ggatccatca
    12001 cctgatagcc aaataccgtc gcgccttgcg tgcgcgccgc aacatgacgc aacttcggac
    12061 tgaagccctg tccgaagaag atgttatcgc ccagcaccag acaagaaggt tcaccgttga
    12121 ggaaggtttc accgatgata aaggcctgcg ccagaccgtc cggactgggc tgttcggcat
    12181 attccagctg gataccgaac tcactaccat cgcccagcag gcgctggaaa taacctttat
    12241 cttccggcgt agtgatgatg agaatttcgc ggataccggc cagcatcagc accgacagcg
    12301 gatagtaaat cattggctta tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa
    12361 tcggatgcaa tcgggtgccg gaaccgcccg ccaggataat acctttcatg tttgcctccg
    12421 ctgaaaatta gcctttcaga cctaaacgct cgccctgata gctgccgtcc tgcacctgct
    12481 tccaccagct ttcattagcc agataccact gcaccgtttt acgcattcca ctttcaaagg
    12541 tttcctgcgg cagccagcca agttcacggg caattttcga agcatcaatg gcataacgca
    12601 gatcatgccc cggacggtca gcgacaaagg tgatcaggtc atgatagtgc gccacgccgt
    12661 gcggcttgtt cggagccagt tcttccagca gctcgcaaat ggtttccaca acatcgagat
    12721 tcttacgctc gttgtgacca ccaatattat aggtttcacc gactttcccg gtggtcgcca
    12781 cgcaatacag cgcgcgggcg tgatcttcca catacagcca gtcacggatt tgctgcccgt
    12841 tgccatatac cggcagcggt ttacccgcca gcgcgttgag gatcatcagc gggatcagtt
    12901 tttccggaaa gtggtaagga ccgtagttat tcgagcagtt ggtgataagc gtcggcagac
    12961 cataggtccg cagccaggcg cgcaccaggt ggtcgctgct ggcttttgaa gcggaatagg
    13021 ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac
    13081 catatacttc gtcagtggag atgtgatgaa aacggaacgc tgatttttta tcttccgtca
    13141 gcgcattcca gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca atgttggttt
    13201 caacaaacgc tgccgggccg tcaatagaac ggtcaacatg gctttcggct gccagatgca
    13261 tgacacaggc tggctgatgc tcagtgaata cgcgcgccag ttctgcccga tcgcagatat
    13321 caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc
    13381 cggcgtaggt cagcttatcg accactacca ccgcatcgct cgtttcgttg atgatataac
    13441 gcaccagcgc cgagccaata aacccggcac cacctgttat cagaatcttt ctcatcagcg
    13501 ccagactcct ttggcatcga cgacatactg ctgatggacg ttgtcgccat tgataacttt
    13561 gaactgacta tgatcgacca gcatcaccag cacatctgcc gttgccagcg cctcgtcaag
    13621 ctgcgccaga gtacaaagcc cggtcagttt cttcggcaac tggtggatgt taggctcaac
    13681 aaccagcgtt tcgccgctgt gccactgggc gatcagttca gcgatttcca tcgccgggct
    13741 ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca aagcaggcga ttttcagttc
    13801 actggcgcgt ttatcggtag ccgccagaca atcagccacc gccgctttca cctgatcgat
    13861 aacccagaac ggtttgtgat cgttcacttc gcgcgcggta cggataagcc gcgcctgctg
    13921 cgggttctgt gccacaataa accacggatc aacagcgatg cagtgaccgc ccacgccagg
    13981 gccaggctga agaatattga cgcgagggtg acgattcgcc aggcgaatca gttcccagac
    14041 gttaatcccc tgatcggcac aaatcagcga caattcatta gcaaaagcga tattcacatc
    14101 gcggaagctg ttttcggtga gcttacacat ttccgccgtc cgcgagttag tgacgacaca
    14161 ctcaccttcg aggaaaatct tgtacagttc gctggcgcgg tccgagcaaa ccggcgtcat
    14221 gccgccaatc acgcgatcgt ttttaatcag ctcgaccatt acctgtcccg gcaacacgcg
    14281 ttccgggcag taggcaatat tgacgtcagc ctgctcgccc acctgctgtg ggaaagtgag
    14341 atctggacgc atctctgcta accattctgc catcttctcg gttgacccca ccggcgaggt
    14401 ggattcaagg atcaccaacg cgcctttttt cagcactggc gcaatggagc gagcagccga
    14461 ttcaacgtag gtcatatctg gctcatgatc gcctttaaac ggcgtgggta cagcaatcag
    14521 ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt
    14581 ttttacgacg ctcgccaaat caggctcgac gatatggatt tcgccacgat tgatggtatc
    14641 aaccgcgtgt tggttgatat cgacaccaat cacctgtttt tgccgtgagg caaacgcagc
    14701 tgctgtaggc agcccgatgt aacccagtcc gataacagaa atggtcgcaa aactcatagt
    14761 gatatccgat tattttttaa cgcttccaga atgcgagagc atgcctgacc atcaccatac
    14821 gggttatggg cgcggctcat agcttgatat tcgttttcgt cttttaaaag acgcgtcact
    14881 tcctcgacga ttcgctgctt atccgtgccg accagacgta ccgtacccgc cgtcaccgct
    14941 tccggacgct cagtggtatc gcgcattacc agcaccggtt tccccagcga aggcgcttct
    15001 tcctgaatgc cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac
    15061 ggtaaatact cctggggatc gatcagaatg acatttttca catgccccag aatgcgattg
    15121 accggctctc tgacgttcgg gttgagatgc actggataga caatctggat gtcctggtgc
    15181 gtggtggcga tgtttgccag cgcatggcag atttcttcaa agccacgacc gaagctctca
    15241 cgcctgtgac cggtcaccag aatcattttt ttattggggt cgataaacgg gtaatttgcc
    15301 gccagttctg aacgcagcgt gtcgctgctc atcacctggt cacgcaccca taacagtgca
    15361 tcaataactg tattaccggt aatgaagatt cggctgtcag caacattttc acgcagcaag
    15421 ttttgccggg aagtttcggt tggagagaag tgatacatcg ccagatgccc ggtcaatgta
    15481 cggttagcct cttccggcca cggcgaatag agatcgcccg tgcgcagacc agcctcaacg
    15541 tgaccaacag gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt tgtagtagta
    15601 tcgccgtgaa ccagcacgac gtctggtttg aactcggcta gaattggttt tagcccttcc
    15661 agaatccgac aggttatctc tgtcaggccc tgtcctggct gcattatgtt gagatcgtag
    15721 tcaggtacaa tggaaaagag tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg
    15781 acgcaaactt tagcctcaaa aaaaggatct tttgccaacg catgcaccaa cggagccatc
    15841 ttgatggctt ccgggcgcgt accaaataca gtcagtactt tcacatcgat tctcttcgaa
    15901 taagcggcga gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc aacggcgggt
    15961 taatgcgaca ccagccccga tcagcccccc gacaatgccc cacataatca tcaggaaggc
    16021 acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc gataggtctg
    16081 aaaacgcgga tccagggttg gaccaacatt cagggtgttt aacatggccc gattctgatc
    16141 atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag cctggagcat
    16201 tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca catctgtcgc
    16261 actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgttcaatac tgttcatccg
    16321 gcggtcgtag atggctttcg ccacctcttc ctgacgcttc acctgtgctt tcatctggat
    16381 ggtacgcgcc gcccatgcgc ctttcagctc atcattcaga tggctggctg cacgctggct
    16441 ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt cggcaatcag
    16501 cttaacgctg tcattaaccg cgcgggtgaa gtcaccggga ataaacacga tgttgttaat
    16561 catttcatcc agcaacgccg catcggcttt actgttgccc accatccgct gcttgtaata
    16621 gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca taacaaactc
    16681 tttataggct tcgtccatga ccgatggttg gtcggcagaa gccatgtttg aacggacatc
    16741 caggttacgc aaaaattgct gctgtgagta atatcccccc agcatattca ccgttggacg
    16801 atcggtaatc gccgtcgagc tccactcctg acgagcaaaa aacgtatacg ccagcgcgat
    16861 taacgcaaac gccagcccca tgccaataat ccatagcttc ccagcccaca aggtacgaaa
    16921 caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag gcattggttg
    16981 tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt tctacgcagt
    17041 ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttaat gcaatatcca
    17101 tagaggaaga atgctagcaa aaagagcacc agcatgaccc actccgggac aaaatgagaa
    17161 tattctgcca gcaccccaat ggaggcgagc agagctgcgg caagggtaat cagcacaaac
    17221 gcctggcggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg acggtcagga
    17281 gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat atccattagc
    17341 ggaatggcga ttatccacaa agcggtaacc gggctgatgg gatgggtttt gccctgggtc
    17401 gtttcgagca ggatccagat aacggtaaaa ccaatcagcg tactgcccgc atcacccata
    17461 aagactttgt agcggcgacc caggatacca aggttaagca tgatgtatgg caggatggcg
    17521 gcgatcatcg cgaagcacca gattgcgagg ctggtttgcc cgtcgaacca caaaatcata
    17581 ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat gccatcaacc
    17641 atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta gccaaacggt
    17701 ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata aagtttgccg
    17761 aacaccatca taacaatgcc aacagcggcc tgtatggtgg cacggatttt tacgctgata
    17821 tcaaaacggt catccagcgc gccaatgaaa acaagcacac cggcacaagc gagatagaga
    17881 gatgcatgcg gaatatagta atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc
    17941 gaaatccccc caacgagagg tatcaatccc tggtgacgtt tgcggaagtt aggtttatcc
    18001 actaaaccga ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa
    18061 aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc tctcagagaa
    18121 aattattagc agaagtataa ccacgaagac cattattcag aagggaaact ctgctctgaa
    18181 cccaccaacc ttgtgcaaaa aacaaccacg tgattcgtgt tatgcgcaat aaacaaacat
    18241 aactcgtatt tgttataacg ccgttaagat tacagatata aaaacaaaac gccacgtaaa
    18301 cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt
    18361 cattggtctt ggtcattgcc agtttattaa tgaggaattc cattgcatcg atttcgccca
    18421 tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga gtagtgagca
    18481 gctcttcttt acgggtaccg gaacggttgt agtcgattgc cgggaagacg cgtttttcag
    18541 cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa
    18601 cttcgtccat cttggaaccg gtatcgataa gcgcggtagc gatgatggtc aggctgccgc
    18661 cctcttccac gttacgcgcc gcaccgaaga agcgtttcgg acgatgcagg gcgttggcat
    18721 ccacaccacc ggtcagcact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca
    18781 gacgagtgat ggagtcgagc aggatgataa cgtctttctt gtgctcaacc aggcgtttcg
    18841 ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggctca tcaaaggtgg
    18901 aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt
    18961 cgatcagcag aaccatcaac acacaatccg ggtggttgta agcaatgctc tgagcaatgt
    19021 tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac
    19081 gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac
    19141 cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt
    19201 tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat
    19261 agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt
    19321 tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc
    19381 tgtctgcgga acggagaaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa
    19441 agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac
    19501 gcatacgagc caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg
    19561 gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc
    19621 tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac
    19681 tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat
    19741 tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa
    19801 attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg
    19861 actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc
    19921 caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc
    19981 cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg
    20041 cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc
    20101 gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca
    20161 gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt
    20221 caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt
    20281 tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc
    20341 gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca
    20401 gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt
    20461 tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt
    20521 agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca
    20581 ttatcttctc tctcatcccg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat
    20641 tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc gacgcggaac cgctggcgga
    20701 agctaccggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca aacagctgaa
    20761 agtgctggaa agcggcgttg acattctgat tggcaccacg gggcgtttaa ttgactacgc
    20821 caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg aagccgatcg
    20881 tatgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc
    20941 aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc
    21001 gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca
    21061 ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac
    21121 gctgatcgaa gaagagtggc cagacagagc gattattttc gccaacacca aacaccgttg
    21181 tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggtga
    21241 tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtgggg atctggatat
    21301 tctggttgcc accgacgttg ccgcgcgtgg cctgcatatc ccggctgtga cgcacgtctt
    21361 taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg
    21421 tgcaggcgca agtggtcatt ccatcagcct ggcatgtgaa gagtatgcat tgaatttgcc
    21481 tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt
    21541 gatgaccgat ctgcctaaac cgctgcgcct cacgcgcccg cgtacaggca atggtccgcg
    21601 tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaaaatg tcatactcaa
    21661 aatcattcaa actgtatcaa gatggcaaga gaatgaaccc cgatgagctt actgtagtaa
    21721 gtgattcagg tgaaagaatg cagccaacac agagacagat tgaaggatga agagtatggg
    21781 ttccacctcg tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt
    21841 tgtgcgcgag gtggctggaa gcatccagac gctgacgcga attaaacgca aagtgcgtct
    21901 ggctgctggc ctgaacagcg aaaatgccct gtctaatgaa gcaatggagc gcggttggca
    21961 atgtttgcgc ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt
    22021 cgctacggcg acattacgac tagccgtcaa tgcgggtgat tttatcgcca aagcacagga
    22081 aatccttggc tgcccggtac aagtcatcag cggtgaagaa gaagcccgcc tgatttatca
    22141 gggggtggcc cacacaacag gcggtgccga tcagcgcctg gtggtggata taggcggtgc
    22201 cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat
    22261 gggctgcgtc acctggctgg aacgctattt tgccgatcgt aatctggcgc aggaaaattt
    22321 tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg aattacggta
    22381 tcacggctgg aaagtgtgcg ttggcgcttc cggcaccgtg caggcgttac aggaaatcat
    22441 gatggcacag gggatggatg aacgcattac cctggaaaag ttgcagcaac tgaaacaacg
    22501 cgccattcat tgcggtcggc tggaagagct agagattgac gggctgacgc tggaacgtgc
    22561 gttagtgttc ccgagtggtc tggcgatcct gatagccatt tttaccgaac tgaatattca
    22621 gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggta tgttgcatct
    22681 taccgtcgag caggatattc gcagccgtac gctgcgtaat attcagcgcc gctttatgat
    22741 cgatattgat caggcacagc gcgtagccaa agttgcggct aacttcttcg atcaggtgga
    22801 aaatgaatgg catcttgaag caataagccg cgatttgctc atcagcgcct gccagcttca
    22861 tgaaatcggc ctgagcgttg acttcaaaca agcgccgcaa cacgctgctt atctggtacg
    22921 taatctggat cttcccggtt ttacccccgc acagaaaaaa ttgctggcga cgctactgct
    22981 caaccagacg aatccggtcg atctctcatc gctgcatcag caaaatgccg taccgccgcg
    23041 cgtcgcagaa caactctgcc gtttactccg cctggccatc atttttgcca gccgtcgtcg
    23101 tgacgatctc gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct
    23161 tccgcaaggt tggttaaccc aacatccgct gggtaaagag attattgatc aggaaagcca
    23221 gtggcagagc tatgtccact ggccgctgga agtgcattaa tgagtaagtg tcggatgcga
    23281 tgctgacgca tcttttccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc
    23341 agattcgcca gatggctttg ccctttctgc atccgttctt cggcgctgac cactttacgc
    23401 tcctgttccc aaatcaaatc atcctgcggc agctccaata agaatcggct tggttccggg
    23461 cgcaccagtt cgccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg
    23521 gcacgagtaa tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgatg
    23581 ctgctctggt gcggcaaaaa accctcttcc ataccgacca tgtagacata aggaaactcc
    23641 agccctttcg acgcgtgcag agtcatcagt tgtacctgat ccagctcttc ctcgctctcg
    23701 ccgcgctcca tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag cgtcatcggc
    23761 tcatccagtt cgctgccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc
    23821 ttcatgcgca tttcggcagc tttcgggctg ggcgatgttt cgtacagcca ggattcataa
    23881 tccatgccgt ggatcagatc acgcaccgcg gcaatcggct cccgctccgc caggcgctgg
    23941 atttctgcca accagtgagt gaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc
    24001 tgactcaggc ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccac
    24061 tcacccagct ttttcagcgt cgccgggcca atctctcgct tcggcgtgtt aacgatacgc
    24121 agaaatgcgc tgtcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg
    24181 atttcaggac gagagaaaaa cgacgtacca ccagatattt tgtacgggat gcggttttgc
    24241 atcaggaatt tttcaaatac ccgcgactga tggttgccgc gataaagaat ggcataatct
    24301 ttgtactgcg ttttattgac gaagtgatgc gcgatcagct cgccggtgac tctctcggct
    24361 tcatgttctt cgttattcgc gcttaaaact tttagctccg cgccatagcc cagttcggag
    24421 aacagacgct tttcaaagac gtgcgggtta ttggcaatga ggatgttcgc cgctttcaga
    24481 atacgtccgg aagagcggta gttctgctca agcttaatca ccttcagtgc cgggaaatcc
    24541 tgactcagca gcaccaggtt ttgcggacgt gcaccgcgcc aggagtagat cgactggtcg
    24601 tcatcgccaa ctacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagttcatac
    24661 tggctggtgt tggtgtcctg atactcatcc accagcaggt agcgaatttt gttctgccag
    24721 cgctcgcgga cttcttcatt gcgttgcagc agtaacgtcg gcaataaaat cagatcatcg
    24781 aaatcgagaa cgttacaggc tttcaggtgt gcatcataca gcccataaca atgggcaaaa
    24841 atacggtccc gctcgccaat cgcactggct gccgcctggg acggtgtttt gagatcgttc
    24901 ttccagttag agatggtcga aatcagctgt tgcaggagga ctttgtcatc ttcaatcagc
    24961 ccctcggtca gctctttaag caaagcaagc tgatcggtat cgtcaaacaa cgagaagttt
    25021 gctttcatcc caagcgccgc atactcgcgt ttgatgatat ccagccccag tgtgtggaag
    25081 gtggagatca tcagcccacg cgcctctttg cgacccaacg tctgcccgac acgctctttc
    25141 atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg
    25201 caaccgcgga tcagatgggc gattttattg gtgatgacac gagttttacc ggaacccgcg
    25261 cctgccagca ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt
    25321 agacgcatag gtgtattgct caatcttcga acggggggga ggattgtagc agaatcgcat
    25381 ttgaatgtga ccgagtgaaa accgctaacc ggatggctgc ggatgtttaa atcagagagg
    25441 gaaggccgaa accggagttc atcatgaatc cattagactt agaaaatatc gggtcgtaac
    25501 gttgtgttat tactttttca gagtttacta aggagaggtt tactcgtggc gattaatttc
    25561 agccccaagg ttggtgaaat actggaatgc aactttggta actatcctgt tagccagaat
    25621 ggacagttca gcacgacgta ttacgatggc cgcattccac ctgagatgat aaaaaatagg
    25681 ttggttgtcg tgctcaacgg aaaaattaat ggcaacgcat gtatcgtcgt tccattatca
    25741 accactcgcg accatgacaa gttaaatcgt ggaatgcatg ttgaaatagc cagtaatgtt
    25801 atcaatgacc tgcaattctt tgatcagcag atacgttggg ctaaagccga cctggtacaa
    25861 caagtcagtc gaaaccgatt aaacagagca aggacttatc gggggtatct taatcaatgt
    25921 ttgccacatg agttggttgc agacatacag agggcagtca tcaagtcaat caatgccata
    25981 tcgctcataa attgacaata aaatcaccat acccgataat gactctgtag cccatagagg
    26041 ccctaagttt tctccccttg agggacctgc gaagcccgga ttttccgggc ttttgccata
    26101 tatgtccccc ctccctgact gacaatgttt aattcaaggc acgatcatgg caaaaacagc
    26161 agcagcactg catatccttg taaaagaaga gaaactggct ctggaacttc tcgagcagat
    26221 taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc cgtcaggtaa
    26281 acgcggtggt gatttaggtg aattccgcca gggccagatg gttccggcgt tcgataaagt
    26341 ggttttctct tgtccggtac tggagccaac cggtccgctg catacccagt tcgggtatca
    26401 catcattaag gtgctttacc gtaactaaca gcaaggcctt ctccgggaga aggccttgaa
    26461 ctctgaatcg cacttaacct gcaacagcaa tacgtttcat atccgtcata tagccgcgca
    26521 gtttcttacc tacctgctca atcgcatgac tgcgaatcgc ttcgttcaca tcacgcaact
    26581 gcgcgttatc taccgcacct tccggaatag ctttacccag gtcgcccggt tgcagctctg
    26641 ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta ccgtattcag
    26701 cggtatcaga aataaccacg ttcatttcat acagacgctt acgggcgatg gtgttggcaa
    26761 tcagcggcag ttcgtgcagt gattcatagt aagcagactc ttcgatgatg ccggaatcaa
    26821 ccatggtttc aaacgccagc tcaacgcccg cttttaccat cgcgatcatc agtacgcctt
    26881 tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt tcaaacgcgg
    26941 ttttgccggt ttcttcacgc caggtcagca gtttcttatc atcgttggcc cagtccgcca
    27001 tcatgccgga ggagaattcg ccggagatga tatcgtccat atgtttctgg aacagcggtg
    27061 ccatgatctc tttcagctgt tcagacagcg cgtaagcacg cagtttcgcc gggttagaga
    27121 gacggtccat catcagggtg atgccgccct gtttcagcgc ttcggtgatg gtttcccaac
    27181 cgaactgaat cagtttttct gcgtatgccg gatcggtgcc ttcttccacc agcttgtcga
    27241 agcacagcag cgagccagcc tgcaacatac cgcacagaat ggtttgctcg cccatcaggt
    27301 cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga ccaccggttg
    27361 ctgccgccca tgctttagcg atcgccatac cttcgccttt cggatcgttt tcagggtgaa
    27421 cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgtact tcggtgcctg
    27481 ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg cccacttcga
    27541 cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt ggctgtacgg
    27601 tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga tccgcctgcg
    27661 ggatcagttc ttcgtaagta ccaactttaa aaccgttttc ggtcgcttta cgccaggatg
    27721 cgcgcttttc ggcaatcgct tctttacgca gagcgtagga gatatcgaga ccagaatcac
    27781 gcatgttcag gccctggttc agaccctgtg cgccacagcc gacgatgact acttttttac
    27841 cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga catttgccca
    27901 gctgtgccag ctgctggcgc agattcagtg tattgaagta gttagccatg gtgattcctc
    27961 gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca ctatatgaca
    28021 ggaaatttat tgcggaaatt gatatattca caacatcaca ttgcaatttc tgcaacgcca
    28081 acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca tctggcggaa
    28141 agccgccatt ttggccgtag cgcgcgggca atgcacgtta gcccgtctac gctttcgcga
    28201 caaatacagc gcctggaaga agatctcggt cagccgctgt ttgtgcgtga taaccgcacg
    28261 gtaacgctga cggaagcggg cgaagaactg cgcgttttcg cccagcaaac gctgttgcaa
    28321 tatcagcaat tgcgtcacac catcgatcag caagggccgt cgctctctgg cgaattacat
    28381 atcttctgct cggtgaccgc tgcctacagc catctgccgc cgattctgga tcgcttccgc
    28441 gcggaacacc cgtcagtgga gatcaaactc actaccggtg atgcggccga tgcgatggaa
    28501 aaggtggtca ctggcgaagc ggatctggcg attgcgggga aaccggaaac cttacccggc
    28561 gcagtggcgt tttcgatgct ggagaatctg gcggtggtgc tgattgctcc cgcgctgccc
    28621 tgcccggtgc gtaatcaggt atctgtcgaa aagcccgact ggtctacagt gccgtttatc
    28681 atggccgatc agggaccggt acgccgccgc attgaactgt ggtttcggcg taacaaaatc
    28741 agcaacccaa tgatttacgc cacggttggc gggcatgaag cgatggtatc gatggtggcg
    28801 ctcggctgtg gcgtggcgct gttgccggaa gtggtgctgg ataacagccc ggaaccggtg
    28861 cgtaaccgcg tgatgatttt agagcgcagt gatgaaaaaa cgccgtttga gctaggcgtg
    28921 tgtgcgcaaa aaaagcggct gcatgagccg ctgattgagg cattctggaa gattttgccg
    28981 aaccactaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc gtggcaatcg
    29041 tagcccagtt cattcagccg ggtttcaaaa tccggttcat gatcgccaag ctcgaacgcc
    29101 gccagtacgc gcccgtagtc ggtaccgtgg ctgcgataat ggaacagcga gatgttccag
    29161 tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg gaattcgaag
    29221 ctgtagaggc gttcctgcaa cggatgcgat ggacgcccgc caaccatata gcgcacatgt
    29281 agcttcgcca tttcgtcgtc ggagagatca accacgctgt agccaccgtc gttgagcatc
    29341 tgcaaaattt ctttgcgctc ttcgagaccg cggctcaggc gcacgccgac aaaaatgcag
    29401 gcgtttttgg catcagcaaa gcggtagttg aactcggtga ccgagcgccc accaagtaac
    29461 tggcagaatt tgaggaaact gcccttttct tccggaatgg tcaccgccag taacgcttca
    29521 cgctgttcgc ccagttcgca gcgttccgag acgtagcgca gaccgtgaaa gttcacgttg
    29581 gcaccggaaa gaatatgcgc cagccgttca ccgcgaatgt tgtgctgggc gatgtatttt
    29641 ttcatccccg ccagcgccag cgcgccggaa ggttccgcca ccgcgcgcac atcttcgaat
    29701 aaatccttca tcgccgcaca gatcgcatca ctatcgacgg tgatgatgtc gtcgagatac
    29761 tcctggcata aacggaaggt ttcgtcgccg atgcgtttta ccgcgacacc ttcggcgaac
    29821 agtcccacgc ggggcagatc aacgggatga cccgcatcca gcgccgcttt caggcaggcg
    29881 gaatcttccg cttccacggc gattactttg atttgcggca tcagttgttt gatcagcacc
    29941 gccacacccg ctgccagacc gccgccgccg accggtacaa atacgcggtc gagatgggcg
    30001 tcctgctgga gcagttccag cgccagcgtg ccttgcccgg cgatcaccat cggatggtcg
    30061 aacggcggta cccaggtgaa accctgctgc tgtgacagtt cgatcgcttt cgctttcgct
    30121 tcatcgaaat ttgcgccgtg aagcagcact tcgccgccaa agccgcgcac cgcatcaact
    30181 ttgatatcgg cggtggcggt tggcatgacg atcagcgcct tcacgcctaa ccgtgcggaa
    30241 gaaaacgcga caccctgcgc gtggttacct gcagaagcgg taatcacgcc gtgcgctttt
    30301 tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcaacttaaa gctatgaact
    30361 ggctggcgat cttcgcgctt caccagaatc acgttatcga gacgcgacga cagtttttcc
    30421 attttctgta gcggcgtgac ctgcgccgct tcgtagaccg gcgcgcgcag caccgctctt
    30481 aaatattcgg caccttccgg ggtaccggac aggggttgcg agtcagccat tattaacccc
    30541 ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg ctggcgtagg
    30601 cacgcagcgc aaaggagacc tgacgttcgc gatttttcgg cgtccaggct ttgtcgccac
    30661 gagcttcctg cgcttcacga cgcgccacca gttcggcatc gcttacctgt aactgaatgc
    30721 cacggttcgg aatgtcgatg gcaatcatgt cgccgtcttc aatcagacca atgctgccgc
    30781 cgcttgccgc ttccggcgag acgtggccga tggaaagacc agaggtgccg ccggagaagc
    30841 gtccgtcggt gatcagcgcg caggctttac cgagccccat tgatttcagg aagctggttg
    30901 ggtagagcat ttcctgcatg ccggggccgc ctttcgggcc ttcgtaacgg atcaccacca
    30961 catcgccagc gacaactttg ccgccgagaa tcgcttctac cgcgtcgtcc tggctttcgt
    31021 acactttcgc cggaccggtg aatttgagga tgctgtcatc gacgcccgcg gttttaacga
    31081 tgcagccgtt ttctgcgaaa ttaccgtaca gcaccgcaag gccgccgtct ttgctgtagg
    31141 cgtgttccag cgaacggatg cagccatttg cgcgatcgtc atcgagagaa tcccaacggc
    31201 aatcctgcga gaatgcctgg gtggtacgaa tgcccgccgg gccagcgcgg aacatatttt
    31261 ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagcgtttgc ggcaacgtca
    31321 ggccaagtac atttttcaca tcacggttca gtaatcccgc acgatccagc tcgccgagaa
    31381 taccgataac accaccagcc cggtgaacat cttccatatg gtatttttgg gtgctcggcg
    31441 caactttaca cagctgcgga accttgcggg aaagcttatc gatatcactc atggtgaagt
    31501 cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac ggtgttggta gagccgccca
    31561 ttgcgatatc caacgtcatg gcgttttcaa acgccgcttt gctagcaata ttgcgcggca
    31621 gtgcactttc gtcattttgc tcgtagtaac gtttggtcaa ttcaacaatg cgtttaccgg
    31681 cattgaggaa cagctgctta cgatcggcgt gagttgccag cagcgagccg ttgcccggct
    31741 gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac atcccggagc
    31801 aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag tcagaaactt
    31861 tcgggtctgc gccctggatc atcgcatcaa ccagatcgag cttgatgatc tgatcggaca
    31921 gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga atattcaggc
    31981 gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg cagaccatgg
    32041 cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt tcgcgagatg
    32101 gcagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc gcaatggtgt
    32161 tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt ttaccaagat
    32221 cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc gcgataatcg
    32281 gcttaccgaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga gcacccgcca
    32341 tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat ttactcccag
    32401 tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta ttgattaact
    32461 tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa gaaggcttgc
    32521 tgaatgcgtt tggtaaccgg gccgcaacgg ccttcaccaa cctgaatacc gtctacactg
    32581 cgcactggcg tgatttctgc cgcagtaccg gacataaaca cttcatccgc caggtacagt
    32641 gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc cagtttgatg
    32701 atggcgtcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt gaacagcaca
    32761 ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc gttcacatcc
    32821 agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag cagggaagag
    32881 aggtagttac caccggcttt tgccgccgtc gggatggtgt ttggtgctgc gcggttccag
    32941 gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cacccagata ggccccccac
    33001 gggaaagcgg caataatcac atcggttgag tatcccgctg gcgggtttac gcccatgcca
    33061 acatcaccaa cgaagatcag cggacggata taggcgctgg tgagattgtt tttgcggatc
    33121 acgtcacgac aagcttccat cagctcatca atgctctgcg aaaccgggaa gcgatagatt
    33181 ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac cggtcctttg
    33241 tgcgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag cgcgtgcgac
    33301 atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca aatgtaatca
    33361 gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt gttgtgatgt
    33421 ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta actgactaaa
    33481 cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat tttgtgcatc
    33541 gctggcggcg gccatattca ttgaacagac gtggaaacca cgatgacgca ccacgcgtaa
    33601 aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat gttgcatcat
    33661 gataatttct ccaacatttc tgaattactg gcaccaggcg gcaccagcgg ccagacgttc
    33721 tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag catggtgtcg
    33781 agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc gaaggcgctg
    33841 gctaacatga ggaaatcggg gttatcagta agggttgttt cgctgtatcg ttcctggaaa
    33901 aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa gacgattttc
    33961 aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat gaaagagccg
    34021 tcaccggaga tacagacgac ggtatcgttc ggtcgcgcga cttgtgcgcc aaccgccgca
    34081 ggtaaaccaa aacccatggt gcctaagccg ctggaggtga tgaaattttc cgggcgagtg
    34141 tgggcgatgt gctgggcagc ccacatctgg tgctgcccca catctgtggt cacgacgcaa
    34201 tccgcaggtt ttcgatccga cagttgtttt aacaacaacg gcgcgtagat agcgtcaccg
    34261 ggatggtcgt aacgccaggt atgttcatca cgcagctgcg cgcagtattg ctgccagtca
    34321 ttgatattta acggctgctg taatgctggt aaaagagcat ttaaatcacc ctgtaaagcc
    34381 acatgtacct gacgcagctt gttcatttct gccggatcga tatccatatg gataacgctg
    34441 gcgtgtggtg cgaaggtgtt cagtttgcca gtcacccggt catcaaaacg tgcacccacg
    34501 gcgatcagca gatcgcactc ctgcactgcg aagttcgccg ctttggtgcc gtgcattccc
    34561 agcatgccca gatagtacgg ataatccgct tcaactgcgc ccagcccttt cagcgtgcag
    34621 gtgacaggca tttttgtggt agcgagaaat tcacgcaaag ccggaactgc ctgcgccatg
    34681 ccaacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat ctggcgcgct
    34741 tgctcgactt cggcatgggg gaaagtcact tcgttttcaa cggtggtgaa ccacggttcc
    34801 agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg accaggacga
    34861 cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc cagcgactgc
    34921 accagaaagc tgtgcttggt acaggctaat gacaatccca gtacatccac ttcctgaaat
    34981 gcgtcagtgc cgataaacgg tgcagacacc tgaccggtga tggcaacgac agggatggaa
    35041 tctaacagtg cgtccgcaag cccggttatc aggttggttg cacctggacc agacgtggcg
    35101 atacatacgc cagttttgcc ggtagcacgg gcataaccga tagccgccat tgctgcacct
    35161 tgctcgtgtc ggcacagcaa gtgttccacg ccaccgtcat acaatgcatc gtaaaccggc
    35221 ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg caacgcatgt
    35281 accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag aaacagaatt
    35341 ttgtgctgtt attcattgtc tgctcctcag ttatgttttt aaggtcaaaa aaaacccccg
    35401 gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct ttcctcgtcc
    35461 aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga ccaggctaat
    35521 cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg aatgcctaaa
    35581 gagttaccat agattttgcg aatagcacaa gatatt
//
LOCUS       DARNVM010000029        35336 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.029, whole genome shotgun sequence.
ACCESSION   DARNVM010000029 DARNVM010000000
VERSION     DARNVM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.029"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..236)
                     /locus_tag="SI621_001351"
     CDS             complement(<1..236)
                     /locus_tag="SI621_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HEI4064084.1"
                     /translation="MAISSYSTVTDKFATLTKMQDENLNEKNRTQKYSGKIGLSVDDV
                     GKYSVTHKDITNILAGDSQSFLKRSLWEFILDLFP"
     gene            complement(663..1388)
                     /gene="phoU"
                     /locus_tag="SI621_001352"
     CDS             complement(663..1388)
                     /gene="phoU"
                     /locus_tag="SI621_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEI4064085.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(1403..2176)
                     /gene="pstB"
                     /locus_tag="SI621_001353"
     CDS             complement(1403..2176)
                     /gene="pstB"
                     /locus_tag="SI621_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEI4064086.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2267..3157)
                     /gene="pstA"
                     /locus_tag="SI621_001354"
     CDS             complement(2267..3157)
                     /gene="pstA"
                     /locus_tag="SI621_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEI4064087.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3157..4116)
                     /gene="pstC"
                     /locus_tag="SI621_001355"
     CDS             complement(3157..4116)
                     /gene="pstC"
                     /locus_tag="SI621_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HEI4064088.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4203..5243)
                     /gene="pstS"
                     /locus_tag="SI621_001356"
     CDS             complement(4203..5243)
                     /gene="pstS"
                     /locus_tag="SI621_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEI4064089.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAITKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5557..7386)
                     /gene="glmS"
                     /locus_tag="SI621_001357"
     CDS             complement(5557..7386)
                     /gene="glmS"
                     /locus_tag="SI621_001357"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEI4064090.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7548..8918)
                     /gene="glmU"
                     /locus_tag="SI621_001358"
     CDS             complement(7548..8918)
                     /gene="glmU"
                     /locus_tag="SI621_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEI4064091.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQADKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(9271..9690)
                     /gene="atpC"
                     /locus_tag="SI621_001359"
     CDS             complement(9271..9690)
                     /gene="atpC"
                     /locus_tag="SI621_001359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEI4064092.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9711..11093)
                     /gene="atpD"
                     /locus_tag="SI621_001360"
     CDS             complement(9711..11093)
                     /gene="atpD"
                     /locus_tag="SI621_001360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEI4064093.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(11120..11983)
                     /gene="atpG"
                     /locus_tag="SI621_001361"
     CDS             complement(11120..11983)
                     /gene="atpG"
                     /locus_tag="SI621_001361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEI4064094.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(12034..13575)
                     /gene="atpA"
                     /locus_tag="SI621_001362"
     CDS             complement(12034..13575)
                     /gene="atpA"
                     /locus_tag="SI621_001362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEI4064095.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(13588..14121)
                     /gene="atpH"
                     /locus_tag="SI621_001363"
     CDS             complement(13588..14121)
                     /gene="atpH"
                     /locus_tag="SI621_001363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEI4064096.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(14136..14606)
                     /gene="atpF"
                     /locus_tag="SI621_001364"
     CDS             complement(14136..14606)
                     /gene="atpF"
                     /locus_tag="SI621_001364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEI4064097.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14668..14907)
                     /gene="atpE"
                     /locus_tag="SI621_001365"
     CDS             complement(14668..14907)
                     /gene="atpE"
                     /locus_tag="SI621_001365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEI4064098.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14954..15769)
                     /gene="atpB"
                     /locus_tag="SI621_001366"
     CDS             complement(14954..15769)
                     /gene="atpB"
                     /locus_tag="SI621_001366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEI4064099.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15778..16158)
                     /gene="atpI"
                     /locus_tag="SI621_001367"
     CDS             complement(15778..16158)
                     /gene="atpI"
                     /locus_tag="SI621_001367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEI4064100.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16775..17398)
                     /gene="rsmG"
                     /locus_tag="SI621_001368"
     CDS             complement(16775..17398)
                     /gene="rsmG"
                     /locus_tag="SI621_001368"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEI4064101.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(17462..19351)
                     /gene="mnmG"
                     /locus_tag="SI621_001369"
     CDS             complement(17462..19351)
                     /gene="mnmG"
                     /locus_tag="SI621_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEI4064102.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19730..20173)
                     /gene="mioC"
                     /locus_tag="SI621_001370"
     CDS             complement(19730..20173)
                     /gene="mioC"
                     /locus_tag="SI621_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HEI4064103.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(20263..20721)
                     /gene="asnC"
                     /locus_tag="SI621_001371"
     CDS             complement(20263..20721)
                     /gene="asnC"
                     /locus_tag="SI621_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HEI4064104.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20873..21865
                     /gene="asnA"
                     /locus_tag="SI621_001372"
     CDS             20873..21865
                     /gene="asnA"
                     /locus_tag="SI621_001372"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEI4064105.1"
                     /translation="MKTAYIAKQRQISFVISHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(21870..23321)
                     /gene="viaA"
                     /locus_tag="SI621_001373"
     CDS             complement(21870..23321)
                     /gene="viaA"
                     /locus_tag="SI621_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HEI4064106.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(23315..24811)
                     /gene="ravA"
                     /locus_tag="SI621_001374"
     CDS             complement(23315..24811)
                     /gene="ravA"
                     /locus_tag="SI621_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HEI4064107.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            25034..26902
                     /gene="kup"
                     /locus_tag="SI621_001375"
     CDS             25034..26902
                     /gene="kup"
                     /locus_tag="SI621_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HEI4064108.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            27069..27488
                     /gene="rbsD"
                     /locus_tag="SI621_001376"
     CDS             27069..27488
                     /gene="rbsD"
                     /locus_tag="SI621_001376"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEI4064109.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            27496..29001
                     /gene="rbsA"
                     /locus_tag="SI621_001377"
     CDS             27496..29001
                     /gene="rbsA"
                     /locus_tag="SI621_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HEI4064110.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFAREQATQEVLMAAAVGKLNRVNQE"
     gene            29006..29971
                     /gene="rbsC"
                     /locus_tag="SI621_001378"
     CDS             29006..29971
                     /gene="rbsC"
                     /locus_tag="SI621_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEI4064111.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            29996..30886
                     /gene="rbsB"
                     /locus_tag="SI621_001379"
     CDS             29996..30886
                     /gene="rbsB"
                     /locus_tag="SI621_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HEI4064112.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            31012..31941
                     /gene="rbsK"
                     /locus_tag="SI621_001380"
     CDS             31012..31941
                     /gene="rbsK"
                     /locus_tag="SI621_001380"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEI4064113.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWVSVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            31945..32937
                     /gene="rbsR"
                     /locus_tag="SI621_001381"
     CDS             31945..32937
                     /gene="rbsR"
                     /locus_tag="SI621_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HEI4064114.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(32903..34330)
                     /gene="mdtD"
                     /locus_tag="SI621_001382"
     CDS             complement(32903..34330)
                     /gene="mdtD"
                     /locus_tag="SI621_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HEI4064115.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(34353..35045)
                     /locus_tag="SI621_001383"
     CDS             complement(34353..35045)
                     /locus_tag="SI621_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEI4064116.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         8572 a         9081 c         8975 g         8708 t
ORIGIN      
        1 ggaaaaagat cgagtataaa ttcccaaaga cttcgtttaa ggaaagattg actatcacca
       61 gctaaaatgt ttgttatatc tttatgagtt actgaatact tacccacatc atcaacagac
      121 aaaccaattt ttcctgaata tttttgcgtt ctattttttt cattcagatt ttcatcttgc
      181 attttagtca gagtagcaaa tttatctgta actgtactat atgatgatat agccataatc
      241 atttctcctg aacacaattt ttattcgccc gctcacaata cataaccgtt ttgtataaaa
      301 aagaacactt tggtgttgtt ttgatacgat tatgactaaa ccacctatga atactgtaaa
      361 aaatcgctca ttttttatgt tccgatatag ggcttttacc ctggtttttt gttttctttt
      421 ccattttctg tgcgggtaac gatataaatg aaaggaataa caaaaaaaat tttataacgg
      481 ggcactacat taaaagtaaa ataattcaaa tgaaataata tatttgcatt gcactgggga
      541 gagtttcgag gtgagtttgt gtggcaatat ttcagggaga aagtgtatca gcccggtaac
      601 tatgtatgac cgggctgatt gccagattcg gcgtaaaagc cttatctgac ctacagttag
      661 cgttactctt tcgggtcttt tccggccagc agtttatcca gctcatcacc gcctacatga
      721 cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg
      781 ccgatacgtt cgattgaacg cgcacagaat agcgcggtaa gtacgctcgg aatggtgcgc
      841 gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtactc ctgatcgact
      901 tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc
      961 agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac
     1021 ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg
     1081 ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagacg caggtcgctc
     1141 gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc
     1201 atgttgacgt tcttgtcgcc ttcgatgacg cgcttcgcca gatcgctgtc ctggttatgc
     1261 atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt catcacctgc
     1321 gtgcggatgc tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga
     1381 ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct tctgtttgtt
     1441 tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt
     1501 acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtgacgatca
     1561 ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg
     1621 ggtcgagtgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac
     1681 gcgcaatgca cagacgctgt tgctgaccac cagagagaga gtaaccgctc tgatgcaatt
     1741 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
     1801 tgtcggcacg ggagagcttc tcaaacagac gcacaccaaa ggcgatgttg tcgtagatgg
     1861 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagggcga
     1921 tatcctgaga gttggtcagg atgttgtcgc catcgagcag aatttcacct tccgcacgct
     1981 gttccgggta cagttcaaac attttattga aggtacgcag cagcgtcgat ttaccgcagc
     2041 cggacggccc gataaacgcc gttacctggt ttttagcgat atccaggttg atgtttttca
     2101 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
     2161 tttcaaccat actcattgca atcttttcct catcgtgcct gatgcacttc gctaatcagg
     2221 cttaccgtag gccggatcag gcgttcacgc cgcatccggc aaagtgttaa ccgtgtttat
     2281 tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag gacgcacagg gtaatgatca
     2341 ataccccggc ccaggccagt tgctgccatt ccgcaaacgg actcatcgca aacttaaaga
     2401 tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga
     2461 gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc agcaggatac
     2521 cggtcataat cccggacacc gacgctttca gcgtaatcgc ggagatcatc ttccacttcg
     2581 gcgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct
     2641 cggtggtgcg gataacaatc ggcacctgca acagcgccag cgcaatcacg cccgcccagc
     2701 cggagaagtg ctccatctgc gccaccacga tggtgtaaac aaacagacca actacaatcg
     2761 acggtgcgga gagcagaatg tcgttaataa agcgaatcac ttctgccagc caggatttac
     2821 gaccatattc cgccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg
     2881 cccacaaaat taacagcccg ctacccgcca gggcgttcgc cagaccaccg ccttccgtat
     2941 tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat accatcgata ccgcgagtga
     3001 tggtggacat taaaatccag atcagccaga acaggccgaa ggccatcgtc gccattgaga
     3061 gcgtcagcgc aatacggttt ttgaggcgac gacgcgcctg cattttgcgg cgagattcag
     3121 ccagcgccgc agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc
     3181 caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat
     3241 caggcccagt tccatcagtg cggcaacgtg cagaccggat tccgcttcgg caaattcgtt
     3301 cgccagcgca gaggtgatac tgttgcccgg catgtacagc gaggcgctgt cgagctgata
     3361 ggtgttaccg atgataaagg tcaccgccat ggtttcaccg agcgcacgcc ccagccccag
     3421 catgatgccg ccgataaccc catttttggt gaacggaaga acgatacgcc agataacttc
     3481 ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa
     3541 cacatcgcgc attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc
     3601 cgcaaggata ccgataccaa atgcggggcc agagaacagc gcgccgacaa tcgggatgtt
     3661 cgacatgata ttgccgaccg gctcctggaa gtaaacggcg aacagcggcg caaagataaa
     3721 caggccccac atgccgtaaa cgatacttgg aatggcagcc agcagctcaa tggcgatacc
     3781 cagcgggcgt ttcagccagc caggcgcaag ctcggtcagg aacagggcga taccaaaact
     3841 caccggaacg gcaatcagca gcgcgataaa cgaggtcacc agcgtaccgt agatcggcac
     3901 cagcgccccg tagatatcgt tcggcgcatc ccactctttg gtccacagga aagccagacc
     3961 aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat
     4021 caatagcaca atcagcgccg ccagttttac tagcacgctg aaaattatgt cgcccttttt
     4081 acccggtggg ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt
     4141 ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt
     4201 tattagtaca gcggcttgcc gctactgtct ttaatattgg tcttccacgc agcgcgaacc
     4261 tgctcaacta cgctatccgg caggctggca taatccaggt cgttcgcctg tttagccccg
     4321 gttttgtatg cccagtcgaa gaacttcagc acttcggtgc cttgttctgg tttcttctga
     4381 tctttatgga tcagaatgaa cgtggtagag gtaataggcc atgcatcttc gcctttctgg
     4441 ttggtcagat cctgagcgaa tgttttgctc cagtctgcgc cttttgcagc gttagcgaag
     4501 ttttcttcgg tcggcaaaac aggtttacca tcagcggaga tcagtttggt gtacgccagg
     4561 ttgttctgct tcgcgtaagc atattcaacg taaccaattg cacctggcag acgctgaacg
     4621 aacgcggcga taccgtcgtt acctttaccg cccagaccaa tcggccattt tacggtagag
     4681 cccgtaccaa cgttgttttt ccactcttcg ttcactttcg ccaggtagct ggtgaagacg
     4741 aaggacgtcc cggagccatc tgcgcggcgt actacagcaa tgttctgtga cggcagtttt
     4801 aagcctggat tcagtttggt gatggcttca tcatcccact tcttgatttt gccgaggtag
     4861 atgtcaccaa gggttttacc atccagcacc agctcgccag acttcaaccc tggaatgtta
     4921 accgccagta ccacgccgcc aatcacggtc gggaactgga acaaaccttc ctgagccagt
     4981 ttttcgtcag acagcggcgc gtcagaggca ccgaaatcaa cggtatttgc gataatctgt
     5041 tttacgccac cggaagaacc gataccctgg tagttaactt tattaccggt ttctttctgg
     5101 taagtgtcag cccatttggc atacaccggc gcagggaagg ttgcacctgc acctgtcagg
     5161 cttgcttctg caaacacaga gaaagcactc atcgataagg tcgcggcgac aacagttgcg
     5221 acggtggtac gcataacttt cataatgtct cctgggagga ttcataaagc attgtttgtt
     5281 ggctacgaga agcaaaatag gacaaacagg tgacagttat atgtaaggtt tatgacagtt
     5341 ttatgacaga gagagaaagt ctttagtctg atttaaataa gcgttgatat tcagttaatt
     5401 acaaatatta ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta
     5461 gccgaagatg acggtttgtc acatggagtt ggcaggattt ttgattaaaa acataacagg
     5521 aagaaaaatg ccccgcttac gcagggcatc catttattac tcaaccgtaa ccgatttcgc
     5581 caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc gcaacgtggt aagccagcag
     5641 ctgcagcgga acggtgtaga agatcggtgc aatcacctct tccacatgcg gcatctcgat
     5701 gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga tcggcgaaga catacaactg
     5761 accgccacgc gcgcgaactt cttcaatgtt ggatttcagt ttttccagca attcgttgtt
     5821 cggtgcaacg acgataaccg gcatatcggc atcaatcagc gccagcggac cgtgtttcag
     5881 ttcgcctgca gcgtaggctt cagcgtgaat gtaagagatc tctttcagct tcaatgcgcc
     5941 ttccagcgcg attgggtact gatcgccacg gcccaggaac agcgcgtgat gtttgtcaga
     6001 gaaatcttct gccagtgctt caatgcgttt atcctgagac agcatctgct caatacggct
     6061 cggtagcgcc tgcagaccat gcacgatgtc atgttcaatg gaggcatcca gacctttcag
     6121 acgagacagc ttcgccacca gcatcaacag cacggttaac tgggtggtga atgctttagt
     6181 ggacgccacg ccgatttctg tacccgcgtt ggtcattagc gccagatcgg attcgcgtac
     6241 cagcgaagaa cccggaacgt tacagattgc cagtgaacca aggtaaccca gctctttcga
     6301 cagacgcagg ccagccaggg tatccgcggt ttcgccagac tgtgacaagg tgatcatcag
     6361 gctgttacga cgcacggcag atttgcgata gcggaattca gaggcgattt cgacgtcgca
     6421 cggaatacct gccagcgatt caaaccagta gcgggaaacc ataccggagt tataagaagt
     6481 accacaggcg aggatctgaa tatgctcaac cttcgacagc agttcgtcgg cgttcggtcc
     6541 cagctcgctt aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt ttttgatcgc
     6601 gttcggctgt tcgtagatct ctttctgcat gtagtggcgg taaatacctt tatcgcccgc
     6661 gtcatattgc agattggatt cgatatcctg acgttttact tccgcgccag ttttatcgaa
     6721 gatgtttacc gaacggcgag tgatttccgc aatatcgccc tcttcaagga agataaagcg
     6781 acgagtcact ggcaacagcg ccagctggtc agaagcgata aagttttcgc ccatacccag
     6841 gccaatcacc agcggactac cagaacgtgc cgccagcagg gtatccggat gacgggagtc
     6901 catgatcact gtaccgtacg caccacgcag ttgcgggata gcacgcagaa cagcctcacg
     6961 cagagtaccg ccttgtttca actcccagtt caccagatgg gcaatcactt cggtgtcggt
     7021 ttcagaaacg aaggtatagc cacgcgcttt taactcttca cgcagcggtt catggttttc
     7081 gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg cgttagcttc
     7141 tgaaggttca ccgtgggtcg cccagcgagt atgagcaata ccggtgccgc catgcagagg
     7201 atgttcttcc gctgcctgag ccagcatctg gactttaccg aggcgacgca ggcgggtcat
     7261 atgaccttct gcatcaacaa cggccagacc ggcagagtca tatccgcggt attcaagacg
     7321 acgtaaacct tcaagaagga tttctgctac atcacgttgc gcgatcgcgc caacaattcc
     7381 acacatagtt tttgattccg atttatatcg ttgtcggtca acctgtatgc ccgttttttc
     7441 gggcaccccg agccttgtag agagtggggt tatttttata gttactgctt gtgggcggga
     7501 gattatgtta tctcctcatc ccatatgacc gtattacccg gccagaatta ctttttcttt
     7561 accggacgac gccagccttc tttttgagtc tgcggcacac ggctgatcgc cagggcgttt
     7621 tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg tcgcgccttt gcctactgtt
     7681 accggggcca ccagctgagt gtcggaaccg acaaacacat cgtcaccgat aatggtctta
     7741 aatttattcg caccatcgta gttgcaggta atggttcccg cgccgatgtt aacgttatcg
     7801 ccaatttccg catcgcccag gtaagtcaga tgaccagctt tcgagccttt acccagacgc
     7861 gcttttttca tctcaacgaa gttaccgacg tgtgcacctt ccagcaactc agcaccagga
     7921 cgcagacggg caaacgggcc aatggtacag gccgctgcca gattcgcatc ttccacaacg
     7981 gtatacgggc taatttcgca atcatcgcca atcacgctgt ttttaatcac gcaaccggtg
     8041 ccgattttca cgcgatgacc gagagtcacg ttgccctcga tgataacgtt agtatcaatt
     8101 tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa aacgcgccgg atcgcgcagc
     8161 ataacgcctg ctaacagcag tttatcagcc tgttcggact ggtaaacgcg ctccagtcgg
     8221 gagagttgca ggcggttatt cacgccttct acttcgctta aacgttgcgg atgaacggcg
     8281 acgatttcac gcccttcctg atacgccagc gcaataatgt cggtgatgta gtattcaccc
     8341 tgagcgttat tgttggtcag cttcgccagc cagcgtttca tatctgcgcc gttggcaatc
     8401 agaatgccgg tgttgatctc ctgaatctga cgctgctcgt cggtggcgtc tttgtgctca
     8461 acaatgccgg taactttgcc gttttcacgg gtgatacgtc cataaccggt cggatcatcc
     8521 agttttaccg tcagcagacc aatgccaccc tgcggtttag catcacgcag gcgctggagt
     8581 gtttcgacag agatcagcgg cacgtcgccg tagagcatta aaatgtcttc atcatcggca
     8641 aagaaaggtg cggcctgctg catcgcatga cctgtaccca gctgttctgc ctgaagcacc
     8701 cagttcaggt tgtcatcttt cagcgcctgt ttaagcagat cgccgccgtg accgtacacc
     8761 aggtgaacgt gcgccgcgcc taattcattc gcagcatcaa tgacatgctg aaccatcgct
     8821 ttcccggcaa gggtatgcag cactttcgga agatcggaat acatgcgcgt gcctttgcct
     8881 gcggcaagga tcactacgct catagcatta ttcaacatac gcgtcctgac tgtaatttga
     8941 gaacgaattt aaaccgcttc accttgaaaa aactacattt ttttcatcgt gaaatggaca
     9001 gaggataaat tgttcaataa cggattatcc ccgctgacga aagtgccatt ttcgaccatc
     9061 gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc atttaagcgt caggtggatg
     9121 tttttgctct tatttcgatc aatgaataaa cgggaaaaca gggtgtttta tatcagcagg
     9181 atctatgtga acgctcttca ggacgggtca atacgcgtaa aaaaaagcca gcctgttacc
     9241 agactggctt ttgtgctttt caagccggtg ttacatcgct tttttggtca actcgataac
     9301 gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc tgagcgtaat ctacgtcgcc
     9361 gtgagagctg ctaatgtgct cttcagcctt acgtttcgct tccatggctc gcgcttcgtc
     9421 gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc acgttgccag gctgcacttc
     9481 aagaatgccg ccagacagat agataaactc ttcgtgaccg tgctgtttca cgatgcgaat
     9541 cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca gggtagatcc ccagttcacc
     9601 ttcgctaccc gttacctgga ttttctcgac cagacctgag aacatttgtt gctctgcact
     9661 gacgacgtcc aggtggtaag tcattgccat atcaccctcc gattaaggcg ttaaagtttt
     9721 ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt agaacgcctg ctccggcagg
     9781 tgatcgtatt cgccttccat gatgccttta aagccacgga tggtgtcttt cagggagacg
     9841 tatttacccg gagaaccggt gaatacttct gccacgaaga acggctggga caggaagcgc
     9901 tggatcttac gagcacgcgc taccaccagt ttgtcttctt cagacagttc atccataccc
     9961 aggatggcga tgatgtcttt cagttcctga taacgttgca ggatggactg aacgccacgc
    10021 gcggtgtcgt agtgttcctg accaaccacc agcgggtcca gctgacggct ggtggagtcc
    10081 agcgggtcaa cggccgggta gatacccaga gacgcgatct gacggctcag taccacggtt
    10141 gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag tcaagtcatc cgcaggtacg
    10201 tataccgcct gtacggaggt gatagaacca gttttggtgg aggtgatacg ttcctgcaga
    10261 acgcccatct cttccgccag ggtcggctga taacctaccg ctgaaggcat acggcccagc
    10321 agtgcggata cttccgtacc ggccagggtg taacgataga tgttgtcaac gaacagcaga
    10381 acgtcacgac cttcgtcacg gaatttctca gccatggtca gaccggtcag agcaacgcgc
    10441 agacggtttc ccggcggctc gttcatctgg ccatacacca gggatacttt gtcgataacg
    10501 ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac gagtacgttc acctacgccc
    10561 gcaaacacag agtaaccgga gtgctcgatc gcgatgttac gaataagctc catcatgttt
    10621 acggttttac ctacgcccgc accaccgaac agaccaactt taccgccctt agcgaacgga
    10681 cacatcaggt cgataacttt gataccggtt tccagcagtt cctgagagtt tgacagctct
    10741 tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt cttcaccgat ctcgcctttc
    10801 atgtcgaccg gttcacccag tacgttcatg atacggccca gagtcgcttt acctaccggg
    10861 acttcgatcg ggtgttcgag gtcttttaca tccagaccgc gacgcagacc gtcggaggaa
    10921 cccattgcga tggtacgcac gataccgccg ccgagctgct gctgaacttc cagcaccaga
    10981 cgctcattac cattttgcac ctcaagagca tcgtacacgc gcggtacggc atcctgaggg
    11041 aattcgacgt caactacggc gccgattacc tggacaatct ttccagtagc catcttaaat
    11101 cctctacgaa ataacctgtt taaaccgcgg cggcccccga gacgatctcg gtgagttcct
    11161 gagtaatgct ggcctgacga gctttgttgt ataccaactg cagctcttta atcaggctgc
    11221 cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc ggcctgctcg ctggccaggt
    11281 tttcaaccac gccctgataa acctgagatt cgacataacg acgcagcagg gtatccagca
    11341 acgccttcgg atcgggttcg tacaggtaat cccaggattt gtgtttcaga tcatcatcat
    11401 ctgatgccgg taacggcagc agctggctga tggtcggaac ctgagacatg gtgttaataa
    11461 atttgttgct gacaatgtaa agcttgtcca gacggccttc gtcgtaggcc tgcaacatca
    11521 cttttaccgg accgatcagt tcggacaggg aagggttatc ccccatgcca gtgacctggg
    11581 caacaacatt gccgcccacg gagttgaaga acgacacgcc tttcgagccg atcattgcga
    11641 ggtcgcattg aacgcctttg tcggtccagg tcttcatttc cgccagcagt tttttgaaca
    11701 ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga caccaccagg tagcccacgc
    11761 gtttaacgtc gcggtcttcc aggtaagggt gcttatattc cagattaccg tgtgcaaggt
    11821 gaccaatcac tttgcgcatg gtttctgcat aaggacggct ggccgccatg cgatcctgcg
    11881 atttacgcat tttggaagcg gcgaccatct ccatcgcttt agtgatcttt tgcgtgttct
    11941 ggacgcttgc gatcttacta cgtatctctt ttgcgccggc catgagcttc tcctcaatgc
    12001 cttgcggcct gccctaaggc aagccgccag acgttaccag gattgggttg ctttgaagga
    12061 atcgaggatg cctttcagct tgccttcgat ttcgtcgttg tagccaccgg tctggttgat
    12121 ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc agcagagcgg cttcgaagct
    12181 gccgattttc gacagttcaa catccgccag gtaaccacgt tctgctgcga acagaaccag
    12241 agactgttgc gctacggaca tcggcgcata ctgtttctgt ttcagcagtt cggtcacttt
    12301 ctgaccgtgg tcaagctgct tacgtgttgc atcgtcaagg tcggatgcaa actgagagaa
    12361 cgctgccagt tcacgatact gtgccagagc ggtacggata ccaccggaca gttttttcat
    12421 gatcttggtc tgtgctgcac caccaacacg ggatacggaa atacccgggt taaccgcagg
    12481 acgaataccg gcgttgaaca ggttggtttc caggaagatc tgaccatcgg taatggagat
    12541 tacgttggtt ggaacgaacg cagaaacgtc acccgcctga gtttcgataa tcggcagcgc
    12601 agtcagagaa ccggttttcc ctttcacttc acctttggtg aaggcttcaa cgtattcggc
    12661 gttaacacgc gcagcacgct ccagtagacg agagtggagg tagaatacgt cgcccgggaa
    12721 tgcttcacgt cctggcggac gacggagcag cagggagatc tgacggtaag caacagcctg
    12781 tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg cggtcacgga agtattcgcc
    12841 cattgcgcaa ccagcgtacg gtgccaggta ttgcagtgca gcggattcag acgcggttgc
    12901 taccacaacg atggtgttag ccagtgcgcc gtgctcttcc agtttacgta ccacgttaga
    12961 aatggtggac gctttctggc cgatagcgac atagatacat ttgataccgg aatcgcgctg
    13021 gttgatgatg gcatcgatag ccaatgcggt tttaccggtc tgacggtcac cgatgatcaa
    13081 ttcacgctga ccacgaccga ttgggatcat ggagtcaacg gctttataac cggtctgtac
    13141 cggctgatct acggactgac gttcgataac gcccggagcg attgcttcta cagcagagaa
    13201 gccgtcgtga tccagcggac ctttaccgtc gattggtgca cccagagtgt taaccacacg
    13261 gcccagcagg ccacggccaa ccggaacttc caggatacgg ccagtacact taactttcat
    13321 gccttcggca aggtcagcgt acggacccat aacaaccgca cctacagagt cgcgctcgag
    13381 gttcagtgcg atagcgtaac ggttacccgg cagggagatc atttcaccct gcatacaatc
    13441 ggccaggccg tgaatgcgga taacaccgtc acttacagaa acaatagtac cttcgttgtg
    13501 agcttcactc acaacattga actgagcaat gcgctgcttg atcagttcgc tgatttcggt
    13561 ggaattcagt tgcatgctcc agtcccctta agactgcaag acgtctgcaa ggcgctcaag
    13621 acgaccgcgt acgctgccat caatgaccat atcacccgct cggatgataa cgcctgccat
    13681 tacagactta tcgattttgc aattcagctt aactttgcgt gacagacgtt tttccatcgc
    13741 agcagaaatt ttcgcgagct gttgttcact cagtgcggca gcggaaatga cgtctacctc
    13801 agcggtagcc tcactcacgg cacgcaggtg aataaactgc tccagaacat ccgggagcgc
    13861 gttaagacga ccattttcag ccattacccg aatcaggttc tgaccgtttt cgtccagttg
    13921 ctcaccacaa actgcgataa acgactcggc gagcgtttct ggcgcaagcg cgccagagag
    13981 aagctctgcc atttgttcgt ttttggttac ctcggcggca aacgccagca tgtcctgcca
    14041 gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct gctttggcgt aggggcgagc
    14101 taccgtaata aattcagaca tcagcccctc cctccttaca gttcagcgac aagtttatcc
    14161 acgatgtcgc tgttagcagc ttcatccacg gaacgttcga tgatcttctc ggcgccagca
    14221 acagccagga tagcaacttg cttacgcagc tcttcacggg cacgtttacg ctcggcttca
    14281 atttccgcct gcgcctgggc cacgatttta gtacgttcct gttctgcctc agctttcgct
    14341 tcgtccagaa tctgcgagcg gcgtttgtta gcctgctcga tgattacctg ggcttccgct
    14401 ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa ggtcaaggtc cttatgtgct
    14461 cgttctgcgg aagcaagacc gtcagcaatt tctttttgac gtttttcgat ggctgccatt
    14521 aatggcggcc atacgtactt catgcagaac agaacgaaca ggacaaacgc gatggcctgg
    14581 ccgaggattg ttgcgttaag attcacagca caatgcctct atttagttaa cgttctgata
    14641 ttgctcttta aataaaagca acgcttacta cgcgacagcg aacatcacgt acagacccag
    14701 acctacagcg atcatcggga tagcatccac cagacccata acgataaaga actgagtacg
    14761 cagcagagga atcagatcag gttgacgcgc tgcgccttcc aggaatttac ccccgaggat
    14821 gccgataccg atcgcagcac cgattgccgc cagacccatc atcacagcgg cagccatgta
    14881 cagcagatcc atattcaggt tttccatgac agtctccagt ttgtttcagt taaaacgtag
    14941 tagtgttggt aaattaatgt tcttcagacg ccatcgacag atagacgatc gtcagaacca
    15001 tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa aatggcccac ggcacattca
    15061 gaatccactg tgaccaccac ggcaacagac cagcaatcag aatgaaaatc agctcaccgg
    15121 catacatgtt accgaacagt cgcaaaccga gtgaaactgg tttggacagc aggcttaccc
    15181 cttcaaggat taagttgaca ggaatgaacg cccagtgatt gaacggctgc agcgtcaact
    15241 ctttcgtgaa gccgccgatg cctttcattt tgatgctgta gaacagaatc aggataaata
    15301 cacccagtgc catagacagc gttacgttca cgtccgcaga cggaaccaca cgcagtgcag
    15361 gcagacccag tacatgttca gcaatgtacg gcagcaggtc gataggcagt aaatccatca
    15421 ggttcatcag gaatacccag acgaagatcg tcagggccag cggagcaatc agcttgcttt
    15481 tgccatggta catgtctttc acgctaccat taacaaagcc gatcaccagc tcaatcgcgg
    15541 tctgaaactt acctggcaca ccgctggtcg cctttttggc tacgctacgg aataaaacca
    15601 ggaacaacag acccagcacc accgagaaga acatggagtc aatattgatt gtccagaagg
    15661 tggctggggg gttatgtgga tccaccagcg agaatgtacg caggtccagc tgaaggttgt
    15721 tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc tgaagccatg atgcctttta
    15781 ccctttgttg ttaattacag ccggtgccag tatctgaacc accagcacca aaacccacgt
    15841 aacgatcagc ggcaagaata ccgcctttaa aaccgccaac gccaccacca gtaacaccaa
    15901 catcgccaga actttgaaag cttcgccaaa tgcgaatgtc caggccaccc ggcctttcgc
    15961 tggtgtatgc gcctggtgac gccaggcaaa tatcataaac aaaacgttag gcagaaagac
    16021 tgccaggccc ccgcttattg cagagacgcc ccagaagggg tctttgaggc tgaacagcaa
    16081 tccacttgct atcaccacca gtaactgaac gagcagaagc ttccgagcaa cgtttcgact
    16141 cacgagcgac acagacatca cgtttttcac tcctgctccc ttcgaggtat gccgcgtgtc
    16201 gtataaaact ttctttaagg cttagagtca agcatcaaaa agcggtcaaa ttatacggtg
    16261 cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa taaatgttta aatatttttc
    16321 cagtgcatac aattgcgact tttctgcaaa ccctgttcga tcatgaaaaa ctgtaaataa
    16381 cgcgtaaaca ctggtgataa agcgtgcttt agatcacata ttgagcatgt tcgcgcacag
    16441 catatttatt tacttggcaa atgatgcctt tgcaagttta tgatatttca gtctaaaaac
    16501 agatactgtt ttaataaatg acatttacac aacaaaaacc acccattgac atttttaata
    16561 atgttttaac agccaatgat gattcttagc gccgattttt agcagactga tattttcact
    16621 tatgacttat tttctgctta ccaaaaaaag ccacgttact ttgttgatgc aaaagagtga
    16681 acgtggcgtt aaatgtaacc agttatatca gtagaaaacc tggttgttgt taacagtcta
    16741 accggtcaat tttttatgat ttttttgata aaaattaaat tttatttgct ttaatcacca
    16801 ccagatgacg ttcgccatcc agggctggaa cctgaagttt aaccactgat tcgacctgat
    16861 attcttcggg caacaaagcg atttcatctt ccggcatttg ccctttcagc gcgtagaaac
    16921 ggccttgctc accaggaaga tggtggcacc agctcaccat atcgttcaga gaggcaaaag
    16981 cgcggctaat tacgccatca aatggcggct ctgaaggaaa ctcttctacc ctgctctgta
    17041 ctggctcaat attctccagt ttaagctcat gttgcacctg acgaaggaaa cgcacgcgtt
    17101 taccaaggct atccaacaac gtgaagtggg cttcaggacg cacgatagag agtggaatgc
    17161 caggcagtcc tggtccggtg ccgacatcga taaaccgttc accttgcaga tacggtgcca
    17221 ccacaatgct atcgagaata tggcgtacca gcatctcatt aggatcgcgg accgaagtca
    17281 ggttgtacgc tttattccat ttatgcagca tattcacata ggcaataagc tggtttttct
    17341 ggtgatcggt aagcgaaata cctgcgtctt tcagcagtaa ggagagtttg ttgagcacgg
    17401 tgattacctg ttcttgatgc gttgcccggt aagcgggtgc ttaccaggca tttttaatgc
    17461 gttatgcgct acgacgcagc ataccctgtt ttttcagcca taccagcaga atggagatgg
    17521 ccgcaggcgt gacgccagaa atacgcgaag cctggccgat agaggccggt ttgtgatcgt
    17581 taagtttggc gatcacttcg ttagaaagac cggatacctg gcggtaatcc agcgtcgctg
    17641 gtagcagggt gttctcgtta cgcagctgct tttcgatctc atcttgctgg cgcgcgatat
    17701 aaccttcgta tttaacctga atctcaacct gttccgccgc ctgttcgtct gtcaacgcag
    17761 gggcaaacgg cgtcagcgtg gttaattttt cataagtcat ttccggacga cgcagcagat
    17821 cttcaccact ggcttcacgg gaaagcggcg cagtcaggtg agcattcact tcagctgcag
    17881 cttccgccga cggggttacc caggtcgatt tcagacgctg acgctcacgc tcgatattct
    17941 caagtttctc gttaaagcgc gcccaacgtt cgtcatccac caggcccagt tcacgaccga
    18001 tttcagtcaa acgcagatcc gcattatctt cgcgcagcat cagacgatat tctgcgcgcg
    18061 aagtaaacat acgatacggt tctttggttc ctaaagtgca caggtcatca accagtacgc
    18121 cgagatacgc ctgagaacgc gccggagccc aaccttcttt gtcagcagac agacgggcag
    18181 cgttaagacc agccagcaaa ccttgcgcag cggcttcttc gtaaccggta gtgccgttaa
    18241 tctgaccagc aaagaacagc ccctggataa acttgctctc cagcgtcggt ttcaggtcgc
    18301 gtggatcgaa gaagtcatac tcaatggcat accccggacg tacgatcttc gcgttttcca
    18361 tcccctgcat ggagcggacg atttgcatct gcacatcgaa cggcaggctg gtggagatac
    18421 cgtttggata aatttcgttt gaggtcagcc cttccggttc aaggaagatc tgatgctgat
    18481 ttctgtcggc gaagcgcatg actttgtctt cgatcgacgg gcagtagcgt gggccgacac
    18541 cttcgatcac ccctgcatac attgggctac gatcgaggtt actgcggatc acatcatggg
    18601 ttttctcgtt ggtatgggtg atataacacg gcacctgctg tggatgctgg gacgcattac
    18661 ccataaacga gaataccggc attgggttat cgccatgctg ttgcgccagc acgctgaagt
    18721 cgatagtacg cgcatcaata cgcggcggtg tcccggtttt cagacgacca acgcgcagcg
    18781 gcagttcacg taaacggcga gagagcggaa tggacggcgg atcaccagca cgaccaccgc
    18841 tgtagttatc cagaccgata tgaattttac cgtcgaggaa cgtcccaacg gtgagcacga
    18901 cggctttggc acggaacttc agtcccattt gggtaacagc accgaccacg cgatcgtttt
    18961 cgacaataag atcttcaacc gcctgctgga agatcatcag gttcggttgg ttctccagcg
    19021 ccgtacgtac cgcctgccgg tagagcacac gatccgcctg agcacgggta gcgcgaaccg
    19081 ctggtccctt gcttgcgttt agtatcctaa actggatacc cgcctgatcg atcgctttcg
    19141 ccatcagacc gccgagtgca tccacttctt ttaccagatg tcccttccca ataccgccga
    19201 tcgccgggtt gcagctcatc tgccccagag tgtcgatatt gtgtgtcaaa agcagagtct
    19261 gttgacccat acgcgccgcg gccatcgcgg cctcggtgcc tgcatgaccc ccgccaatga
    19321 tgatgacgtc aaaaggatcc ggataaaaca tggtgattgc ctcgcataac gcggtatgaa
    19381 aatggattga agcccgggcc gtggattcta ctcaactttg tcggcttgag aaagacctgg
    19441 gatcctgggt attaaaaaga agatctattt atttagagat ctgttctatt gtgatctctt
    19501 attaggatcg cactaccttg tggataacaa ggatccggct tttaagatca acaacctgga
    19561 aaggatcatt aactgtgaat gatcggtgat cctggaccgt ataagctggg atcagaatga
    19621 ggggttatac acaactcaaa aactgaacaa ccgttgttct ttggataact accggttgat
    19681 ccaagctttc tgacagagtt atccacagta gatcgcacga tctgtatact tatttgagta
    19741 aattaatcca cgatcccagc cattcttctg ccggatcttc aggaatgtcg tgatcaagga
    19801 tgttgatctt cagagtttcg cctgtctgtt ttgcaccgga atttttgagt tctgcctcga
    19861 gtttatcgat agccccacaa aaggtgtcat attcacgact gccaataccg atagcgccaa
    19921 agcggaccgc agaaagatcg ggcttctgtt cctgcaatgc ttcatagaaa ggagaaaggt
    19981 tgtccggaat atctccggca ccgtgggttg agctgataac cagccagatc cccgaggcag
    20041 ataaatcttc caacagcgga ccgtgcagcg tttcggtggt aaaacccgcc tcttccagct
    20101 tttcagccag gtgttctgct acatattcgg caccgccgag ggtgctgccg ctgataagag
    20161 tgatatctgc cataaaccgc cacctttatt aagagtggcg tattgtacgc tgtgaacgcg
    20221 ttgggatcta cctgtggaaa agtatgggat taaaaaagcc gatcagggct tgatggtacg
    20281 catgatcggg ttctgcagga cgatcagtgt ctcggtggac tgaatttcat caattgtttg
    20341 gatcttgttg ataagtacat gctggagagc atcgatcgaa cggcacatca ctttgataaa
    20401 gatgctgtag tggccagttg tgtaataggc ttcagtgact tcatcgaggc tttccagctt
    20461 tgccagcgcg gaaggatagt ctttggcgct ctttaatata atgccgataa agcagcctac
    20521 gtcataaccg agctgcttcg ggctgacatc aatacgcgcc ccggtaatga tccccgcctg
    20581 cttcattttc tctactcgaa cgtgaattgt ccccggactg acgccaaatt gtttcgccag
    20641 ttcggcgtaa gcggtgcgcg cattgcccat taatgcttcc aggatgccac ggtccagatt
    20701 gtcgatcaga taattttcca taggattttc ttatgaggat tgatgattca ttctatttta
    20761 gccctatttt ttaatgaatc aaaagtgcat gaggattttt attgaatgat tattgcatgt
    20821 gtgtcggttt ttgttgctta atcataagca acaggacgca ggagtataaa aaatgaaaac
    20881 cgcttacatt gccaaacaac gtcaaattag cttcgtgata tctcactttt ctcgtcaact
    20941 ggaagaacgt ctggggctga tcgaagtcca ggcaccgatt cttagccgtg tgggggatgg
    21001 cacgcaggat aacttgtcgg gctgtgaaaa agcggtgcag gtaaaagtaa aagctctgcc
    21061 tgatgcccag ttcgaagtgg ttcattcact ggcgaagtgg aaacgtcaga ccttagggca
    21121 acacgacttc agcgcgggcg aagggctgta cacgcacatg aaagcccttc gccccgatga
    21181 agaccgtctt tctccgttgc actcggtcta tgtagaccag tgggactggg aacgcgtaat
    21241 gggcgacggc gagcgtcaat tctcgactct gaaaagcacg gtagaggcga tctgggcggg
    21301 aattaaagca accgaagctg cggttagcga agagtttggc ctggcaccgt tcctgccgga
    21361 tcagatccac ttcgtacaca gccaggagtt actgtctcgt tatccggatc tcgatgccaa
    21421 agggcgtgag cgggcgattg cgaaagatct tggcgcggta ttcctcgtcg ggattggcgg
    21481 caagttgagt gatggtcatc gtcacgacgt gcgcgcaccg gattatgatg actggagcac
    21541 cccgtcagag ctgggccatg cgggtctgaa cggcgatatt ctggtatgga acccggtact
    21601 ggaagatgcg tttgagcttt cttccatggg gatccgcgtg gatgccgata cgttgaagca
    21661 tcagctggcg ctgaccggtg acgaagatcg cctggagctg gagtggcatc aggcgctgct
    21721 gcgcggtgaa atgccgcaga ccatcggcgg cggtatcggg cagtctcgtt tgaccatgct
    21781 gctgctgcaa ctgccacata tcggccaggt tcagtgtggt gtatggccgg cagctgttcg
    21841 cgagagcgtc ccttctctgc tgtaataatt tatcgccgcc agcgtctgag caggcggctt
    21901 cgcatcccgg tatcaaagcg ccagatatga tcgaaaatgc gcatgatgcc gggtttgcca
    21961 tgtgccgaca tcgccacggc atgaaagcga tgctgatgta cccgctgtag ctctttcact
    22021 ttactcgtca cgtcgtcagg caaccgctgg gcgataaaat cagaaatcac caccgcatcg
    22081 gcatcaaacc attcccggct ttgcaagcgt tccataatgg cgcgaaaaca actggccaga
    22141 tcggtgccgc cacgaaactg ctggcttaaa aaacggattg cttgttcgat gccttgtggg
    22201 cctgaaagct cataacggac gatctcggtg gaaaataaca taatatagca gcgccggttt
    22261 tcagcgagag caatgcgcat caaggccagg cagaacgctt tcgcacactg ttcattaaag
    22321 ccgcccattg agccggaagt atccacacag acaataaacg gtccgcgcgg ctgttcgtcg
    22381 taatctttat gtaccaccgg acgttcgatc actttttctc gccacgactc accgtgcagg
    22441 cgataggtga gcaactgttt ttccaccagc cgacggtaaa actcatactc cagttctgtt
    22501 atccctagtg tcgccagttc tggcggcagg agacgtaaaa tatcatcgct ttgttgcaga
    22561 ccatcaacct gctcaggaac cgtcgccggt tcgcgcacca gggtgcggaa ggtttccatc
    22621 tgcgcatcgt tgcgcggtat tgatttggct tcccgggaac gccccagctg ttctgccagg
    22681 cgtttcagtt ccggctgttc gttaagaaat tcaccgtatt tcacaatcaa ctgatagtcg
    22741 ccacgtttaa gctgaccggc gctcatatcc cacagacgac cagctgcggt attgttatct
    22801 gcgagaatcg gttcaagttg tccgctcagc gtcatgcgtt cctgaacttc actcaacagt
    22861 tgttcgcgtt cttcttctaa tagctgttga tttaacgtcg ttgcttgcac gatcagactt
    22921 aaacgccaac gctggagaaa aagcgtgtgt aacgctgaag tgatcgtgct gttagcatca
    22981 accaactgtc gggcttgttc tgcccatggg gaattcagac gatgcagtaa gtccaggatc
    23041 tgtggtagct gcacaataaa ctgcggcgtg gagaggagct ggctttgctg atagcacatc
    23101 acctcttcgg tgagttccgg cgggactcgg gcatctttca gccgactgcg cagcgcctca
    23161 cgccagcggg gaacatcatc agtgattgcc gccttcagtc gtgggaattt ttcaaagaag
    23221 actgccagct gcggtgaggc cagcagcgcg atgatcatct cttcgatcaa tccctcttcg
    23281 ctgacggcca gcatcacatt aagcgtatcc agcgttagca ttgttgtgcc tggcgaatct
    23341 gtgcgccgac atcctgtagg ctggcttcaa tgcgacctaa ccagtcgcca gggataaaca
    23401 gacatttttg ctgttcgctg aataaagcgt gttgcttgcg ccagtcgctt tcaagctctt
    23461 ccagttgttg ttttatttca cctggcagac cttcagccga tgaaccggga agtgccagcg
    23521 tactgccttg taaacttaca tcgcgtacaa caagatgttg ggcgctatcc acttccagat
    23581 tcagtttctg ggcaaagccg ataccgttca gtttgccgcg aatctcacca cctttactca
    23641 gccactgttc cagcgcgcta cgctcaaagg agatatgaac cacttccata tcatgcaatt
    23701 ttaacggttt ttgcagcagc agagtcagag tggaagcagt aacgttaaca ggaagttgat
    23761 actgctgacg acggctgaaa atgccgccca gacgaattac cgttaaggct gttttatcgc
    23821 tttgttgctg ctggagttgc aggtgacgtt gcacaatcgc gccaaggcgg gtcaacatcc
    23881 cttgctgttg ccaggcatga ccggtcatca atacatcaat ttgttgttgt atcaaattca
    23941 ggctttgcgc gtcataccac aggcaatctt tcagcaaaat gagatcaacc ggggcaacag
    24001 cactgcgacc gctaaaaaag gcgctggcct gcaataatcg aatcgctttt ttccagcgac
    24061 gatccgagac ataaggcgca tccggtaatt tatccagttg ctggcgcagc ataaaaatca
    24121 gctcaaatac atgatcgggc agcgtaattt caccaatctc tttctgccag cgttcatatt
    24181 cttcatctgt gacctgcaag gaggcaggaa ccggattgtc gttttcatcc tgttgactgg
    24241 tcagcatgga gcggaaattc gctttatcct gcactttatc taaccacaga cgaatcagca
    24301 tgcggtcata taacgcttcc agactgctgt ctgcttccgg cagctcgttg gaggccgcca
    24361 ccagcaggcg catcgggatt ttttctacgt gtgcaccgtt acggaactgg cgctcgttaa
    24421 tggcggtgag cagggtatta agaattgccg ggcccgcttt ccagatctca tccagaaaga
    24481 cgatttctgc ttccggcagg taaccgctgg ttaaacgttc atagcgccct tcatctttta
    24541 gcgcctgaat agaaagggga ccaaaaactt cttccggcgt ggagaagcgg gtcatcagat
    24601 attcaaacgc gcgggcattc tgaaaggcga attttaagcg ccgggcgatc aaacttttgg
    24661 caatacctgg cgggccaagg aggaacacac tttcaccact taatgccgct aataaacaca
    24721 agcggatggc gtggctacgt tcataaagcc ccttttccaa cgaactgctc aggcgggaaa
    24781 ttctttccgc taataaatga gggtgagcca taatgaagtg gcgtcctttc gtcaaaagtt
    24841 ctgcgtaaat tgcgagtata gacgtttctt gctggtggct aaaatagtct caaagggggg
    24901 gtatttttct ttgagccagg ttaatgtggc cgcatttagg agtacgattt tgccgttaat
    24961 cgtgcatact gtgcgctttt ttgtgggcca agggactaag cacacatttc atatttcaac
    25021 gaaagactag tctatgagca ctgataataa gcaatcattg cccgcgatta ccctcgcggc
    25081 gattggagtt gtctacggcg atattggtac cagcccgtta tatacacttc gtgaatgttt
    25141 gtccggccag tttggttttg gcgttgaacg cgatgccgtg tttggctttt tatcgctgat
    25201 cttctggctg ctaatctttg tggtttccat taaatatctc accttcgtga tgcgggcaga
    25261 taacgccggt gaagggggga tcctgacgtt gatgtcgctt gccgggcgca atacgtcggc
    25321 gcgaaccaca tcaatgctgg tgattatggg gctaatcggc ggcagctttt tctatggtga
    25381 agtcgtcata acacccgcta tttcggtgat gtcagccatt gaaggtctgg aaatcgtcgc
    25441 cccgcagctg gatacctgga tagttcctct ctcaattatc gttctcactt tattatttat
    25501 gattcaaaag catggcacgg ctatggtcgg taagctgttt gcgccgatca tgctgacctg
    25561 gtttttgatt ctggctgggc tggggttacg tagcattatt gctaacccgg aagtgctgca
    25621 tgcgctgaat ccgatgtggg cggtgcattt cttccttgaa tacaaaacgg tttcttttat
    25681 tgcattaggg gcagtggtgc tgtcgattac gggggtcgag gcgctgtatg ctgatatggg
    25741 gcactttggt aagttcccga ttcgtctggc gtggttcacc gtcgtattgc cttccttaac
    25801 tcttaattac ttcggtcagg gagcgctgtt gttaaagaac ccggaagcaa ttaagaaccc
    25861 gttcttcctg ttggcaccgg actgggcgct gatcccgctg ctgatcatcg ccgcactggc
    25921 gacggtaatc gcctcgcagg ctgttatctc tggcgtcttc tcattgacgc gtcaggcggt
    25981 acgtctggga tatttgtcgc cgatgcgcat tattcacacc tccgaaatgg agtcagggca
    26041 aatctatatt ccgtttgtga actggatgct ctatgtcgcg gtcgtgattg tgattgtcag
    26101 ctttgagcac tccagcaacc tggcggcggc gtacgggatt gcggtgaccg gaaccatggt
    26161 gctgacgtct attctctcga ctaccgtggc acgtcagaac tggcactgga ataagtattt
    26221 tgttgcgctg atcctgattg ctttcctttg tgtcgatatt ccattgttca ccgctaacct
    26281 cgataaactg ctctccggcg gctggttgcc attgagcctc ggtacagtga tgtttatcgt
    26341 gatgaccacc tggaagagcg agcgtttccg cttgctgcgg cggatgcatg aacatggcaa
    26401 ctctctggag gcgatgattg cttcgctgga gaaatcaccg cccgttcgcg tgcccgggac
    26461 cgcggtgtat atgtcacgtg caatcaacgt cattcccttt gcgctgatgc ataacctgaa
    26521 acataacaag gtattgcatg agcgggtgat tctgttaact ctgcgcaccg aagacgcgcc
    26581 atatgtccat aacgtccgtc gggtacagat tgaacaactg tcgcccactt tctggcgcgt
    26641 ggtggcaagt tatggttggc gagaaacgcc aaacgtggaa gaagtattcc accgctgcgg
    26701 tctggaagga ttaagttgcc ggatgatgga aacctccttc tttatgtcgc atgagtcgtt
    26761 gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggt aagctgtact tgctgttgca
    26821 acgtaatgcg ctgcgtgcgc cggatcaatt tgaaatcccg ccaaacaggg ttatcgaact
    26881 gggtactcag gtcgaaatct aacgccagac gcctcctttc tccataaggg ggcgtttttg
    26941 ttttcatggt taatcaccat gtaaaacgtt tcgaggttga tcacatttcc gtaacgtcac
    27001 gatggttttc ccaactcagt caggattaaa ctgcgggtca gcgaaacgtt tcgctgatgg
    27061 agaaaaaaat gaaaaaaggc accgttctta attctgatat ttcatcggtg atctcccgac
    27121 tgggacatac cgatacgctg gtggtgtgtg atgcaggttt acccatcccc aaaagtacaa
    27181 cgcgtatcga tatggcatta acccagggtg taccttcttt tatgcaggtg ctgggcgtcg
    27241 tcacaaatga aatgcaggtc gaggcagcca ttatcgcgga agagatcaaa caacataatc
    27301 cgcaactcca cgaaacgttg ctcactcacc ttgagcagct gcaaaaacac cagggaaata
    27361 ccattgaaat tcgttacacc acgcatgaac aattcaaaca acaaaccgca gaaagtcagg
    27421 cggtaattcg cagcggagaa tgttctccgt atgcgaatat cattctctgt gctggcgtga
    27481 cgttctgagg ccgtcatgga agcattactt cagcttaaag gcatcgataa agccttcccg
    27541 ggcgtaaaag ccctctcggg tgcagcgtta aatgtctatc ctggccgcgt gatggcgctg
    27601 gtgggcgaaa acggtgcggg taaatccacc atgatgaaag tgcttacggg tatctatact
    27661 cgcgatgccg gtacgctttt atggctgggg aaagaaacga cgtttaccgg gccgaaatct
    27721 tcccaggaag ccgggattgg gattatccat caggaactga acctgatccc gcagttgacc
    27781 attgccgaaa acattttcct cggtcgtgag tttgttaatc gctttggcaa aattgactgg
    27841 aaaaccatgt atgccgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
    27901 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat agccaaagtg
    27961 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
    28021 gaaaccgaat ccttgttccg cgtcatccgc gagctgaaat cgcaaggccg cggtattgtc
    28081 tatatttccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
    28141 gatgggcaat ttattgctga gcgtgaagta gcatcactga ccgaagattc gctgattgag
    28201 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
    28261 attcgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
    28321 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg ctgggcgtac cgaactgatg
    28381 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
    28441 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctctgaagac
    28501 cgtaaacgcg acggtttggt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
    28561 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggcagta
    28621 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
    28681 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acgccccaaa
    28741 gtgttgatcc tcgatgagcc tacccgtggc gtagatgtcg gcgcgaaaaa agagatctat
    28801 caactgatta accagttcaa agccgatggc ttgagcatca ttctggtgtc atcggagatg
    28861 ccagaagtat taggcatgag cgatcgcatc atcgtcatgc atgaagggca tctcagcggg
    28921 gaatttgctc gtgagcaggc cacccaggaa gtgttaatgg ctgccgctgt gggcaagctt
    28981 aatcgcgtga atcaggagta aaaaaatgac aacccagact gtctctggtc gccgttattt
    29041 cacgaaagcg tggctgatgg agcagaaatc gcttatcgct ctgctggtgc tgatcgcgat
    29101 tgtctcgacg ttaagcccga actttttcac catcaataac ttattcaata ttctccagca
    29161 aacctcggtg aacgccatta tggcagtcgg gatgacgctg gtgatcctga cgtcgggcat
    29221 cgacttgtcg gtaggttctc tattggcgct gaccggcgca gttgctgcat ctatcgttgg
    29281 cattgaagtc aatgcgctgg tggctgtcgc tgccgctctc gcgttaggtg ccgcaattgg
    29341 tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag gcgtttatcg ctacgctggt
    29401 tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc aacggtagcc cagtgaatac
    29461 cggctttact gagaacgccg atctgtttgg ctggtttggg atcggtcgtc cgctgggcgt
    29521 accgacgcca gtctggatca tggggattgt gttcctcgcg gcctggtaca tgctgcatca
    29581 cacgcgtctg gggcgttaca tctacgcgct gggcggcaac gaagcggcaa cgcgcctttc
    29641 tggtatcaac gtcaataaaa tcaaaatcat cgtctattct ctttgtggtc tgctggcatc
    29701 gctggccggg atcattgaag tggcgcgtct ctcctccgca caacctacgg cggggactgg
    29761 ctatgagctg gatgctattg ctgcggtggt tctgggcggt acgagtctgg cgggcggaaa
    29821 aggtcgcatt gttgggacgt tgatcggcgc attaattctt ggcttcctta ataatggatt
    29881 gaatttgtta ggtgtttcct cctattacca gatgatcgtc aaagcggtgg tgattttgct
    29941 ggcggtgctg gtagacaaca aaaagcagta ataacgacta caggacatct tgagtatgaa
    30001 catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta agcgccaccg tcagtgcgaa
    30061 tgcgatggca aaagacacca tcgcgctggt ggtctccacg cttaacaacc cgttctttgt
    30121 atcgctgaaa gatggcgcgc agaaagaggc ggataaactt ggctataacc tggtggtgct
    30181 ggactcccag aacaacccgg cgaaagagct ggcgaacgtg caggacttaa ccgttcgcgg
    30241 cacaaaaatt ctgctgatta acccgaccga ctccgacgca gtgggtaatg ctgtgaagat
    30301 ggctaaccag gcgaacatcc cggttatcac tcttgaccgc caggcaacga aaggtgaagt
    30361 ggtgagccac attgcttctg ataacgtact gggcggcaaa atcgccggtg attacatcgc
    30421 gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa ggcattgccg gtacatccgc
    30481 agcccgtgaa cgtggcgaag gcttccagca ggctgttgct gctcacaagt ttaatgttct
    30541 tgccagccag ccagcagatt ttgatcgcac taaaggtttg aacgtaatgc agaacctgtt
    30601 gaccgcacat ccggatgttc aggctgtatt cgcgcagaat gatgaaatgg cgctgggcgc
    30661 gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg gtcgtcggat ttgacggtac
    30721 accggatggc gaaaaagcgg tgaatgatgg caaactagca gcgactatcg ctcagctacc
    30781 cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa gtgctgaaag gcgagaaagt
    30841 tcaggctaag tatccggttg atctgaaact ggttgttaag cagtagtttt aatcaggttg
    30901 tatgacctga tggtgacata aatacgtcat cgacagatga acgtgtaata taaagaaaag
    30961 cagggcacgc gccaccctaa cacggtggcg cattttatgg acatcccgaa tatgcaaaac
    31021 gcaggcagcc tcgttgttct tggcagcatt aatgctgacc acattctgaa tcttcaatct
    31081 tttcctactc caggcgaaac cgtaaccggt aaccactatc aggttgcatt tggcggcaaa
    31141 ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga atatcgcgtt tattgcctgc
    31201 actggcgatg acagcattgg tgagagcgtt cgccagcagc tcgccactga taacatcgat
    31261 atttctccgg tcagcgtgat caaaggcgaa tcaacaggtg tggcgctgat ttttgttaat
    31321 ggcgaaggtg agaatgtcat cggtattcat gccggcgcta atgctgccct ttccccggcg
    31381 ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag cattattaat gcagctggaa
    31441 tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc atcaaaataa gactatcgtt
    31501 gcgcttaacc cggctccggc tcgcgaactt cctgacgaac tgctggcgct ggtggacatt
    31561 attacgccaa acgaaacgga agcagaaaag ctcaccggta ttcgtgttga aaatgatgaa
    31621 gatgcagcga aggcggcgca ggtactgcat gaaaaaggta tccgtactgt actgattact
    31681 ttaggaagtc gtggtgtatg ggttagcgtg aatggtgaag gtcagcgcgt tcctggattc
    31741 cgggtgcagg ctgtcgatac cattgctgcc ggagatacct ttaacggcgc gttaatcacg
    31801 gcgttgctgg aagaaaaacc attgccagag gcgattcggt ttgcccatgc cgccgctgcg
    31861 attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt ggcgtgaaga gatcgacgca
    31921 tttttagaca ggcagaggtg acgcttggct acaatgaaag atgttgcccg cctggcgggc
    31981 gtttctacct caacagtttc tcacgttatc aataaagatc gcttcgtcag tgaagcgatt
    32041 accgccaaag ttgaagcggc gattaaagaa ctcaattacg cgccatcagc cctggcgcgt
    32101 agcctcaaac tcaatcaaac acataccatt ggcatgttga tcactgccag taccaatcct
    32161 ttctattcag aactggtgcg tggcgttgaa cgcagctgct tcgaacgcgg ttatagtctc
    32221 gtcctttgca ataccgaagg cgatgaacag cggatgaatc gcaatctgga aacgctgatg
    32281 caaaaacgcg ttgatggctt gctgttactg tgcaccgaaa cgcatcaacc ttcgcgtgaa
    32341 atcatgcaac gttatccgac agtgcctact gtgatgatgg actgggctcc gttcgatggc
    32401 gacagcgatc ttattcagga taactcgttg ctgggcggag acttagcaac gcaatatctg
    32461 atcgataaag gtcatacccg tatcgcctgt attaccggcc cgctggataa aactccggcg
    32521 cgcctgaggt tggaaggtta tcgggcggcg atgaaacgtg cgggtctcaa cattcctgat
    32581 ggctatgaag tcactggtga ttttgaattt aacggcgggt ttgacgctat gcgccaactg
    32641 ttatcacacc cgctgcgtcc tcaggccgtc tttaccggaa atgacgctat ggctgttggc
    32701 gtttaccagg cgctgtatca ggcagagtta caggttccgc aggatatcgc ggtgattggc
    32761 tatgacgata tcgaactggc aagctttatg acgccaccat taaccactat ccaccaaccg
    32821 aaagatgaac tgggggagct ggcgattgat gtactcatcc atcggataac ccaaccgacc
    32881 cttcagcaac aacgattaca acttactccg attctgatgg aacgcggttc ggcttagatt
    32941 tacgctgtct tttgatcaaa ttattaccat cggttgtttt cagaagcatg aacattgctg
    33001 ctgaagcaac agtaataatg cccattgtga taaacgtata gtggaattgt tcgacagtcg
    33061 ttgtaccttc catcccttca taaacgcgaa ggacggccgc acttacagca acgcctaaac
    33121 tgatagacag ttgctgcgtg accgccagaa cactgttacc gctgctggcg ttgtcatcgg
    33181 tcagatcggc aagtgtgatg gtattcattg cggtaaattg cgtcgacata gccatcccta
    33241 atataaacaa cggcaagatc agcatccata tcgccattgc tggtgattgc aaagagaact
    33301 gagcgatcat tagcccaata atcaccgtga tccccactaa cgtatggcga tagcccagac
    33361 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
    33421 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
    33481 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
    33541 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtgcgt cgtgcatgga
    33601 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggca atccaactgg
    33661 cgacaatctt ttccccgaat agctctattc cgcttgagaa gagaacaagg ctaaggccaa
    33721 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
    33781 tgcgcgcgta aagaaggccc gcaataccta tggggatatt gattaaaaat atccagtgcc
    33841 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
    33901 ccggcatggc gacaaaattc aatacaggaa gaagctcatt acgaggataa gcgcgcagta
    33961 aggccagccg agcaacaggc atcatcattg cgccgcctat cccctgaata acccggaaga
    34021 caaccagctg tggtagcgag ttagaaagtg cgcaggccag agaacccaac gtgaacagac
    34081 tcacggcaag ggtaaatatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
    34141 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
    34201 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
    34261 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgccatactg cgcttctttt
    34321 tatcgctcat tcaatgtcct gttatcgggt tgtcacttat caggtgagcg tagcagcgcc
    34381 tgacaagctt taaatgccgc gtcgccatcg ctttggacaa tcgcatcgac aatcgcctgg
    34441 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
    34501 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
    34561 tcatgccaag ccatatcgac ctcaatccag cgttcacggc gaaagttctc ttttaatgcc
    34621 accatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgca
    34681 gccagtaggc aagcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
    34741 tgatgaaagt tctcttctgt catccaccag gtaagcaatt cctgatcaag aaaattccag
    34801 tttgattgtg gcatgacccg ggtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
    34861 gttaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
    34921 tcaatctcac caggcaaaat ggtgccgggt tcatattcac cttttaagat gcgttgcgcc
    34981 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
    35041 ggcattacta atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
    35101 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg cttttgctga
    35161 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    35221 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct
    35281 cctgagaatc tcaacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaag
//
LOCUS       DARNVM010000030        35257 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.030, whole genome shotgun sequence.
ACCESSION   DARNVM010000030 DARNVM010000000
VERSION     DARNVM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.030"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            242..934
                     /gene="araD"
                     /locus_tag="SI621_001384"
     CDS             242..934
                     /gene="araD"
                     /locus_tag="SI621_001384"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEI4064117.1"
                     /translation="MDTLKEKVLAANLSLPLHQLVTFTWGNVSGIEREKGVMVIKPSG
                     VEYSQMGIADMVVVDLQSGEVIDGHRKPSSDTDTHLALYRAFPDIGGIVHTHSRHATI
                     WAQAGKDLTAFGTTHADYFYGIIPCTRPMKDDEINGQYEYETGRVIIETFRQREISPA
                     DIPAVLVNSHGPFAWGTDPANAVHNAVVLEEIAYMNLFTQQLHPHLTAIQQPLLDKHF
                     LRKHGANAYYGQ"
     gene            complement(984..2357)
                     /gene="argH"
                     /locus_tag="SI621_001385"
     CDS             complement(984..2357)
                     /gene="argH"
                     /locus_tag="SI621_001385"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEI4064118.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVENAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(2418..3194)
                     /gene="argB"
                     /locus_tag="SI621_001386"
     CDS             complement(2418..3194)
                     /gene="argB"
                     /locus_tag="SI621_001386"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEI4064119.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(3202..4206)
                     /gene="argC"
                     /locus_tag="SI621_001387"
     CDS             complement(3202..4206)
                     /gene="argC"
                     /locus_tag="SI621_001387"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEI4064120.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLNLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            4360..5511
                     /gene="argE"
                     /locus_tag="SI621_001388"
     CDS             4360..5511
                     /gene="argE"
                     /locus_tag="SI621_001388"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEI4064121.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5813..8464
                     /gene="ppc"
                     /locus_tag="SI621_001389"
     CDS             5813..8464
                     /gene="ppc"
                     /locus_tag="SI621_001389"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEI4064122.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            8647..10380
                     /locus_tag="SI621_001390"
     CDS             8647..10380
                     /locus_tag="SI621_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HEI4064123.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            10529..11380
                     /locus_tag="SI621_001391"
     CDS             10529..11380
                     /locus_tag="SI621_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4064124.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(11367..11708)
                     /locus_tag="SI621_001392"
     CDS             complement(11367..11708)
                     /locus_tag="SI621_001392"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HEI4064125.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11710..12588)
                     /locus_tag="SI621_001393"
     CDS             complement(11710..12588)
                     /locus_tag="SI621_001393"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HEI4064126.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(12554..14851)
                     /locus_tag="SI621_001394"
     CDS             complement(12554..14851)
                     /locus_tag="SI621_001394"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEI4064127.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAHILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASRKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14902..15222)
                     /locus_tag="SI621_001395"
     CDS             complement(14902..15222)
                     /locus_tag="SI621_001395"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HEI4064128.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(15237..16316)
                     /locus_tag="SI621_001396"
     CDS             complement(15237..16316)
                     /locus_tag="SI621_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HEI4064129.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            16625..19126
                     /gene="ptsP"
                     /locus_tag="SI621_001397"
     CDS             16625..19126
                     /gene="ptsP"
                     /locus_tag="SI621_001397"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEI4064130.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPRIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSEGLSCAEAIVAS
                     AHHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            19138..19800
                     /gene="fsa"
                     /locus_tag="SI621_001398"
     CDS             19138..19800
                     /gene="fsa"
                     /locus_tag="SI621_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEI4064131.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDTIPGIVVKIPVTSEGLAAIKILKKE
                     GITILGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19811..20914
                     /gene="gldA"
                     /locus_tag="SI621_001399"
     CDS             19811..20914
                     /gene="gldA"
                     /locus_tag="SI621_001399"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HEI4064132.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            21245..21862
                     /locus_tag="SI621_001400"
     CDS             21245..21862
                     /locus_tag="SI621_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEI4064133.1"
                     /translation="MKASLALVSLLTAFTSNSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21889..22794)
                     /gene="yijE"
                     /locus_tag="SI621_001401"
     CDS             complement(21889..22794)
                     /gene="yijE"
                     /locus_tag="SI621_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HEI4064134.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSALLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSAVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKNI"
     gene            complement(22887..25067)
                     /gene="katG"
                     /locus_tag="SI621_001402"
     CDS             complement(22887..25067)
                     /gene="katG"
                     /locus_tag="SI621_001402"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEI4064135.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(25396..26286)
                     /gene="metF"
                     /locus_tag="SI621_001403"
     CDS             complement(25396..26286)
                     /gene="metF"
                     /locus_tag="SI621_001403"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEI4064136.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(26668..28221)
                     /locus_tag="SI621_001404"
     CDS             complement(26668..28221)
                     /locus_tag="SI621_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEI4064137.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAKSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            28354..29484
                     /locus_tag="SI621_001405"
     CDS             28354..29484
                     /locus_tag="SI621_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEI4064138.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTFATEKGHFCPVCYGTKTICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITPPEIIETVALLVPFQGASGGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHESNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGVI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGTTYHGWWKNQWQLWVQGKNRKMVCALTGGNNSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCARRLISD"
     gene            complement(29448..31880)
                     /gene="metL"
                     /locus_tag="SI621_001406"
     CDS             complement(29448..31880)
                     /gene="metL"
                     /locus_tag="SI621_001406"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HEI4064139.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(31883..33043)
                     /gene="metB"
                     /locus_tag="SI621_001407"
     CDS             complement(31883..33043)
                     /gene="metB"
                     /locus_tag="SI621_001407"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEI4064140.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            33320..33637
                     /gene="metJ"
                     /locus_tag="SI621_001408"
     CDS             33320..33637
                     /gene="metJ"
                     /locus_tag="SI621_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HEI4064141.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            33697..34305
                     /locus_tag="SI621_001409"
     CDS             33697..34305
                     /locus_tag="SI621_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HEI4064142.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVKQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            34450..35125
                     /locus_tag="SI621_001410"
                     /pseudo
     CDS             34450..35125
                     /locus_tag="SI621_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
BASE COUNT         8562 a         9717 c         8820 g         8158 t
ORIGIN      
        1 atttaaatac aaaaacaaaa ataatcgggc atagcaatct cttccattca tgaccgaata
       61 cgcccgaata aaaccgttta cgcccactta ccctctttca ttgccttctt acatgccttg
      121 aatattttac atttcttata ttcagcgtaa tttaggcaac ataacttacg agcaaaaata
      181 gccaaaaccc aaaagttaaa gtttatttat ataatacata cagattatcg tggagttaaa
      241 aatggatacg ctaaaggaaa aagttcttgc tgcgaattta tcactacccc ttcatcagtt
      301 agtgacgttt acctggggaa atgttagcgg tattgagcgc gaaaaaggtg tcatggttat
      361 taaaccttct ggggtcgaat acagccaaat gggcattgca gatatggtgg tagtggattt
      421 acaaagtggt gaagttattg atggacacag gaaaccctct tcagacacag atactcatct
      481 ggcgctatac cgtgctttcc cggatattgg aggtatcgtt catacccatt cccgacacgc
      541 cacaatatgg gctcaggccg gaaaagatct cacggccttt gggacaaccc atgcagatta
      601 tttctacggc attatcccct gtacacgccc catgaaggat gacgaaatta atggacaata
      661 tgaatatgaa acggggcgag tgattattga aacgttcaga caacgagaaa tatctcccgc
      721 tgacattccg gctgttttag taaacagtca tggccccttt gcctggggaa ctgatcccgc
      781 gaacgccgtg cataacgccg ttgtacttga agaaatcgcc tatatgaatc tctttacaca
      841 acagctacac cctcatctta cggctattca acagcctcta ctggataagc attttttacg
      901 caagcatggt gccaatgctt attacgggca gtaaccctct aagcatggcc tggcgttttg
      961 ccaggccacg aatcgcccag gacttacccc aaccgcgcct gcgcaaaagc aatcgcctgc
     1021 gccacctgct gcggtgagac accgcctttt gccgcacgct tgtcgaggca cgattgcagc
     1081 gacagaatcg gatagacatc ttcgccaatc acctggctga atttctgcaa ctcactgagc
     1141 ggcagatctt ccagcggttt gccctgacga atggcttcca ccaccgcttc acccacaata
     1201 tgatgcgcct cgcggaacgg cacgcctttc gccaccaggt aatccgccag ttcagtggcg
     1261 ttcgcatacc cctgctgcgc cgcttcctgg caacgcggac gcttcacctg aatgccgtcc
     1321 agcaccagcg ccgccatatg caggcagtcc agccaggtat cgagcgcgtc gaacagacct
     1381 tctttgtctt cctgcatatc tttgttgtaa gccagcggca aacctttcag cgtcatcatc
     1441 atgccggtta acgcgccctg cacacgaccg catttaccgc gaatcagctc cagcgcatcc
     1501 gggtttttct tctgcggcat caatgatgaa ccagaagtca cgcggtcaga aagctcgaca
     1561 aaccccgctt cgccggtgtt aaagaaaatc agatcttcag caaaacgcga cagatgcacc
     1621 atgccgatag cggcagcaga aagcagttcc agcacgtggt cacggtcaga aacgctgtcg
     1681 agactgttac gggtcgctga ggcaaatccc agccagcctg ctaactgttc acggtcgatt
     1741 tcataggccg ttcccgccag tgcgccacag cctaacgggc tgacatccag acgcttaagc
     1801 gcatcctgca agcggctttc atcacgcgcc aacatctcaa cataggccag gcaccagtgc
     1861 gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac ctggcatcac cgcgtcctga
     1921 ttgttttgtg cgttttccac cagcgccgat tgcagctgcc ggttagccgt cagcaactcg
     1981 ctaacggtat ctttgcacca cagtttcagg tcagtcgcta cctgatcatt acggctacgc
     2041 ccggtgtgca gttttttgcc taactgaccc actttgtcga tcagtttgcc ttccacccag
     2101 ctatggatat cttcggcgtc gctttcaagg atttgttgtg gcctggcgcg aacatcttcc
     2161 agcagcacgt tcagcgcctc ttccagttgc gcctgttctt ctgcggtcag cacaccgacc
     2221 gttaccaggg ctttggacca ggccacagag ccaacaatat cctgctccgc cagacggtaa
     2281 tcaaaacgca gtgagtcgtt gaattgtttg aaccgttgat ctgctgcctg ggtaaaacgc
     2341 ccgccccaaa gtgccataac tctgtttcct tattttgaaa tttaatgccg gaaagctgcg
     2401 cctccggcta acaaaactta agctaaaatc cgcgtaccca tcggcatacc gttaaacagt
     2461 gccggaagct gttccgcatg acgccaggag gcgatatcca ccgggcggcc cagcgtgcgc
     2521 gcagcatcca gcgccgcgtt cactttcact atcatgccgt cagtaataat gccctgctca
     2581 atcagttgtt ctgctttcgc ggcagtcatt tcggcaatgc gttgcccttt gccgtcgaga
     2641 atgccactga cgtcggagag caaaatcaga tccgcgccca gcgttgccgc cagcgccgtc
     2701 gctgcctggt cagcattgac gttcatcagt tgcccttcgt ctgttacgcc aatggagctg
     2761 accaccggca gataaccgtt ctccagcaat gtgttgataa gcttaggcga acctggctgc
     2821 gccagtccaa catggcctaa ctcttcatca agctgggtca ctttgacgct gtcgccgtca
     2881 ccgagaaaca aacctacggc cgcaatctga tgtttcttcg cccatgccag cagggtttta
     2941 tttgccgttc ccgccagtgc tccggtgata atgtcaatct gatcagcagg cgtcacccgc
     3001 aggccgtttt tctttttcac cggcagattc agccctttca tcagctcatc caccacgcaa
     3061 ccgccgccgt gcacaatcac cagcggacgc tgatgcgact cacgataatt caccagtgcg
     3121 ctaaacagac gttccagcgc ctcttcacta tccagcagta cgccgcccag tttgataatt
     3181 aatggattca tcattgcacc cttaaataag agactgcgtt tccgcatagc cgaaacgaat
     3241 attggcgcac tgtaccgctt gtgccgccgc gcctttcagt aagttgtctt cggtcgccac
     3301 aataatcaaa tgctcgccct gaacggcaaa cccgatatcg caaaatggca gcccaacgac
     3361 atttttcagc gccggaacgc ctttgtcata cagccgcacc agcggtttat gggcatacgc
     3421 ctgctgtaac gcttgcgcga cttgcgcctg ggtcacaccc gatttcaggc ggcaggtaat
     3481 ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg gtgaagataa cgtcagcacc
     3541 gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag acgccatacg gttgcaggct
     3601 aacttcacaa aaactatttg aaatcgctgc cttacgtccc gcaccgctca cgccgctggt
     3661 ggcgttgatc accggccact gattgaggtt aagaagatcg gcatcaatca acggtttcag
     3721 cgccagctgt gccgccgtcg gataacagcc cggcaccgca atcaagttcg cttcttttaa
     3781 tttattaccg caccactccg ccagaccgta ggctgcctgt tccaacagtt ccgggtattg
     3841 atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg ttaacacgaa acgcgccgga
     3901 aaggtcgaac accacgcagc ccgcttcaag aaattgcggc gctaaatcgt ggctaacttc
     3961 atgggcggtg gcgagaaaca ccacgtccac ccctgggcta aactcgctga tatccgacat
     4021 cggctgcaac ggcagatcaa cgatgccttt tagctgcgga tgcaaatcgg agattaactt
     4081 tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt atgttcatat gcggatggcg
     4141 atttacatag gtcactagct ctgcgccagc gtagccgctg gcacccacaa tcagcgtatt
     4201 caacatcggg gctattcacc ttcttatgtc tggttgccag gttaaacgta aaacattcac
     4261 cttacggctg gtgggtttta ttacgctcaa cgttagtgta tttttattca taaatactgc
     4321 atgaatattg atactatcat gaccagaggt gtgtcaacaa tgaaaaacaa attaccgcca
     4381 tttatcgaga tttaccgtgc tctgattgcc acaccttcaa taagcgccac ggaagaggca
     4441 ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg actggtttaa aaatttaggc
     4501 ttcaatgtgg aagtgcagcc tgtgccagga actcgcaaca aatttaatat gctggccagt
     4561 tgcggacagg gggctggcgg cttgttgctg gcggggcata ccgatacggt gccatttgat
     4621 gacggacgct ggacgcgcga tccgtttaca ctgacggagc atgacggcaa gctttacggc
     4681 ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc ttgatgcgct acgcgatgtc
     4741 gacgtcacga aactgaaaaa accgctctac attctggcga ctgccgatga agaaaccagt
     4801 atggccggag cgcgttattt tgccgaaact actgccctgc gcccggattg tgccatcatt
     4861 ggcgaaccga cgtcactaca accggtacgc gcacataaag gtcatatctc taacgccatc
     4921 cgtattcagg gccagtcggg gcactccagc gatccagcac gcggagttaa cgctatcgaa
     4981 ctaatgcacg acgccatcgg gcatattttg caattgcgcg ataacctgaa agaacgttat
     5041 cactacgaag cgtttaccgt gccataccct acgctcaacc tcgggcatat ccacggtggc
     5101 gacgcttcta accgtatttg cgcttgctgt gagttgcata tggatattcg tccgctgcca
     5161 ggcatgacac tcaatgaact taatggtttg ctcaacgatg cattggctcc agtgagcgaa
     5221 cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc cgatccctgg ctatgaatgc
     5281 ccaccgaatc atcaactggt tgaagtggtt gagaaattgc tcggagcaaa gaccgaagtg
     5341 gtgaattact gtaccgaagc gccgtttatt caaacgttat gcccgacgct ggttctgggg
     5401 cctggctcaa ttaatcaggc tcatcagcct gatgaatatc tggagacgag gtttatcaag
     5461 cccacccgcg aactgatcac ccaggtgatt caccattttt gctggcatta aaacgtaggc
     5521 caaacaatgt cggatgcgat gcttgcacat cttatccgac ctacaccttt ggtgttaatt
     5581 gagacgattt tttaacattc ccataagtta cgcttattta aagcgtcgtg aatttaatgg
     5641 cgtaaattcc tgctatttat tcgtttgctg aagcgatttc gcagcatttg acgtcaccgc
     5701 ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc gagcatattc gcgccaatgc
     5761 gacgtgaagg atacagggct atcaaatgat aagatggggt gtctggggta atatgaacga
     5821 acaatattcc gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg gagaaaccat
     5881 caaggatgcg ttgggagaac acattcttga acgcgtagaa actatccgta agttgtcgaa
     5941 atcttcacgc gctggcaatg atgctaaccg ccaggagttg ctcaccacct tacaaaattt
     6001 gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga acctggccaa
     6061 caccgccgag caataccaca gcatttcgcc gaaaggcgaa gctgccagca acccggaagt
     6121 gatcgcccgc accctgcgta aactgaaaaa ccagccggaa ctgagcgaag acaccatcaa
     6181 aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg gctcacccaa ccgaaattac
     6241 ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc tgtttaaaac agctcgataa
     6301 caaagatatc gctgactacg aacacaacca gctgatgcgc cgcctgcgcc agttgatcgc
     6361 ccagtcatgg cataccgatg aaatccgtaa gctgcgtcca agcccggtag atgaagccaa
     6421 atggggcttt gccgtagtag aaaacagcct gtggcaaggc gtaccgaatt acctgcgcga
     6481 actgaacgaa caactggaag agaacctcgg ctacaaactg cccgtcgaat ttgttccggt
     6541 gcgttttacc tcgtggatgg gcggtgaccg cgacggcaac ccaaacgtca ctgccgatat
     6601 cacccgccac gtcctgctac tcagccgctg gaaagccacc gatctgttcc tgaaagatat
     6661 tcaggttctg gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc tggcgctggt
     6721 tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc gttctcgcct
     6781 gatggcgaca caggcatggc tggaagcgcg cctgaaaggc gaagaactgc caaaaccaga
     6841 aggcctgctg acacaaaacg aagaactgtg ggaaccgctc tacgcttgct accagtcact
     6901 tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc tgcgccgcgt
     6961 gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt caggagagca cacgtcatac
     7021 cgaagcgctg ggcgagctga cccgctacct cggtatcggc gactacgaaa gctggtcaga
     7081 ggccgacaaa caggcgttcc tgatccgcga actgaactcc aaacgtccgc ttctgccgcg
     7141 caactggcaa ccaagcgccg aaacgcgcga agtgctcgat acctgccagg tgattgccga
     7201 agcgccgcaa ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc cgtccgacgt
     7261 gctggctgtc cacctgctgc tgaaagaagc gggtatcggg tttgcaatgc cggttgctcc
     7321 gctgtttgaa accctcgatg acctgaacaa cgccaacgat gtcatgaccc agctgctcaa
     7381 tatcgactgg tatcgcggcc tgattcaggg caaacagatg gtgatgattg gctattccga
     7441 ctcagcaaaa gatgcgggcg tgatggcagc ttcctgggcg caatatcagg cacaggatgc
     7501 attaatcaaa acctgcgaaa aagcgggtat tgagctgacg ttgttccacg gtcgcggcgg
     7561 ttccattggt cgtggcggcg cacctgctca tgcggcgctg ctgtcacaac cgccaggaag
     7621 cctgaaaggt ggcctgcgcg tgactgaaca gggcgagatg atccgcttta aatatggtct
     7681 gccagaaatc accgtcagca gcctgtcgct gtacaccggg gcgattctgg aagccaacct
     7741 gctgccacca cctgagccga aagagagctg gcgtcgcatt atggatgaac tgtcagtcat
     7801 ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc cttacttccg
     7861 ctccgctacg ccggaacagg aactgggcaa actgccgttg ggttcacgtc cggccaaacg
     7921 tcgcccgacc ggtggcgttg agtcactgcg cgccattccg tggatcttcg cctggacgca
     7981 aaaccgcctg atgctccccg cctggctggg tgcaggtacg gcgctgcaaa aagtggtcga
     8041 agatggcaaa cagagcgagc tggaggctat gtgccgcgat tggccattct tctcgacgcg
     8101 tctcggcatg ctggagatgg tcttcgccaa agcagacctg tggctggcgg aatactatga
     8161 tcaacgtctg gtagacaaag cactgtggcc gttaggtaaa gagttacgca atctgcaaga
     8221 agaagacatc aaagtggtgc tggcgattgc caacgattcc catctgatgg ccgatctgcc
     8281 gtggattgca gagtctattc agctacggaa tatttacacc gacccgctga acgtattgca
     8341 ggccgagttg ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac cggatcctcg
     8401 cgtcgagcag gcgttaatgg tcactattgc cgggattgcg gcaggcatgc gtaataccgg
     8461 ctaatcttcc cctcctgcaa accctcgtgc ttttgcgcga gggttttctg aaatacttct
     8521 gttctaacac cctcgttttc aatatatttc tgtctgcatt ttattcaaat tctggatatc
     8581 ccttcagata tccttaagga attgtcggta cattcggcga tatttttttc aagacaggtt
     8641 cttactatgc attccacaga agtccaggct aaacctcttt ttagctggaa agccctgggt
     8701 tgggcactgc tctacttttg gtttttctct actctgctac aggccattat ttacatcagt
     8761 ggttatagtg gcactaatgg cattcgcgac tcgctgttat tcagttcgct gtggttgatc
     8821 ccggtattcc tctttccgaa gcggattaaa attattgccg cagtaatcgg cgtggtgcta
     8881 tgggcggcct ctctggcggc gctgtgctac tacgtcatct acggtcagga gttctcgcag
     8941 agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta tttaagccag
     9001 tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcagtggc agttctgctg
     9061 tggacacgcc tgcgcccggt ctatattcca aagccgtggc gttatgttgt ctcttttgcc
     9121 ctactttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa gaacaagccg
     9181 tttgagaaaa cgttggataa cctggcatcg cgtatggagc ctgccgcacc gtggcaattc
     9241 ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagct actgaatgaa
     9301 aataatgcct tgccgccgct ggcgaatttc aaagatgaat cgggtaacga accgcgcacc
     9361 ttagtgctgg tgattggcga gtcgacccag cgtggccgca tgagtctgta cggttatccg
     9421 cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct gaccgtgttt
     9481 aataacgtgg tgacgtctcg tccgtacacc attgaaatcc tgcaacaggc gctgaccttt
     9541 gccaatgaaa agaacccgga cctgtacctg acacagccgt cgctgatgaa catgatgaaa
     9601 caggcgggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc ccgcaatacc
     9661 atgctgacgg tattttcgcg ccagaccgac aagcagtact acatgaacca gcaacgtacg
     9721 cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt tccaggaagt gctgaatgac
     9781 cctgcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa atacaaatac
     9841 cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc gccgggatta
     9901 aacgcggaag agctggagtc atataacgat tatgacaacg ctaacctgta taacgatcat
     9961 gtggttgcca gcctgattaa agactttaaa gcagcagacc cgaacggttt cctggtttac
    10021 ttctctgacc acggtgaaga ggtttacgac acgccgccgc acaaaactca ggggcgtaac
    10081 gaagacaacc cgacgcgtca tatgtacacc attccgttcc tgctgtggac gtcggaaaaa
    10141 tggcaagcga ctcatccgcg tgatttctcg caggatgttg atcgtaaata cagccttgcg
    10201 gaactgatcc acacctggtc agatttggcg ggcttatctt acgacggtta cgatccaacc
    10261 cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa cccgtacaag
    10321 aaaaacgcgc tgatcgatta cgacacgctg ccatatggcg accaggtagg taatcagtaa
    10381 atgacctttg tatcgcgtct ggcgctattt gccggatgcg atactgccgc aatgtttaca
    10441 tcgaactccc ctcaggccac acttttcagg tggccttttt tcataatgcc agttactgtt
    10501 ttcccgtcta catagctaag ggaataacat gtatcacgac gtcagctatc tgctctcccg
    10561 cctgatcaac ggcccgctgt ccctgcgcca gatttacttt gccagcagca acggtccggt
    10621 ccccgatctc gcgtatcagg tcgattttcc acggctggaa attgtgctgg aaggtgagtt
    10681 tgttgatacc ggcgctggag caacgttagt tcccggcgat gtgctgtacg ttcctgctgg
    10741 tggctggaat tttccacaat ggcaagcccc cgccactacc tttagcgtgc tgtttggcaa
    10801 acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa caatatcaaa atctggcgaa
    10861 gcaacacgtc gcccggcgcg gcccacgcat aggttctttt ctgctacaaa cgctcaatga
    10921 aatgcaaatg cagccgcagg agcagcaaac ggcaaggctt atcgtcgcca gcctgcttag
    10981 ccactgccgc gatttgcttg gcagccaaat ccagaccgcc tcacgcagtc aggcactatt
    11041 tgaagctatt cgtgattata tcgacgaacg ctatgcctcc gcgcttaccc gcgaatctgt
    11101 cgcacaggca ttctatattt cgccaaatta cctctcgcac ctgtttcaaa aaacgggggc
    11161 cattggtttt aacgaatacc tcaatcacac gcgactggag cacgctaaga cgttattgaa
    11221 aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg acagcaacta
    11281 cttctgccgt ctgtttcgta aaaataccga acgctcaccg tcggaatatc gtcggcagta
    11341 ccacagccaa ctgacagaaa aaccgactac tccagaatga gatgggtttg ctgcggcgca
    11401 gaaagcactt tgcgtaccgc gctcatcacc cgctgcggct cacgcaggaa agcgtagatg
    11461 ctgcattgca catagcggca atgttcaaat cgctcagcac cggcaagctc gatatccgta
    11521 atcaacagcg caacatccgc ccggcggata tcctcgtccg ctaaacgatt ctccgttccc
    11581 aacgcgccct gggtttcaat gctgacgccc cacttctcta actggcacaa cttttccagc
    11641 cgttccgccg ccatatatgt atgcgccacg ccgcttacgc aggcggttac tgccaccagg
    11701 tatgccattt taacctccca cggtaacctg aaatccggcc cgttctgcca tttcacgcat
    11761 cgttgccaca tcggctgacg acggcgcagc aacctctttc atcgaccatg ttttccccag
    11821 caggcggtat ttcggctcgc cgtactgatg aaacggtaac agatggatct gcttgatatt
    11881 cagcgggatc agcacgtcca gcgcctgctg catattctcc cggctgagcg tgaaaccagg
    11941 gatcaacggt aaacgcggga tcacgttgac gccctcactc accagcaaac gcagattctc
    12001 cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt ccataatttt
    12061 taaatcgaac aacacttcat cgcacaattt cgccagcggt aacagcttgc tggctggcgc
    12121 gtctccggca gtttcaatgg cacatgacac accccacagc cgcagtcgct gtaaaaaacg
    12181 ggtagcaaat tccgcctgca ttaacacttc gccgccagaa agcgtgacgc cgccgccgga
    12241 cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc agcgcgtcaa ggctgatatc
    12301 gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc gcatcacgca aacatttcgc
    12361 acagtgcaga catttcgcct ctctgcgtac agtctggatt ttgccggaga tcgattccgg
    12421 attagcacac cacgggcaaa gatgtggaca gcctttaaaa aagaccaccg tacgaatgcc
    12481 ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat cattgcggcg
    12541 cgtttccaca acgttacagc tgatgcgctg tccggcggat gatgtcatcc tggatctcct
    12601 tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg caccaccagc ccggcataat
    12661 cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta aactgaatat
    12721 gttgtaactt aagctgggta aacgcccgta agaagtcggc cagtttgcgt aatcccgctt
    12781 caccttccag ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta gacagcagcg
    12841 tattatcgag cttactgact gacttcagta ccgccgttgg cccttgtgcg tcctgaccca
    12901 gcataggtga caagccgccg tctgccagct gttccccggc aaaacgaccg tctggcgtcg
    12961 cgccaaccac cgatcccaac gggacgtgag ccgaaacggt atacgatccc ggcgtgaagt
    13021 agccgccgcg cgggttctgg tatttttcca cttctttgca gtagtggcgt aacagttcgg
    13081 cgctgatgtt atccacctcg tcgatatcgt taccgtattt ctcgaagcgg ttaattaagc
    13141 gagcacggac tttttcgcct tctggcgttg cgaagttggc ttttaatacc gacagcaatt
    13201 cgtcaaaact taaacgctgt tgctcaaaaa ccataccttt caacgcatgg agagaatcgc
    13261 tcaggttggc gataccgatc ccctgtacgc cggagaagtt ataacgcgca ccgccatcgg
    13321 taatatcgcg gcctttttcc agacaatcgc tgataaacga tgagagcagc ggtacaggtg
    13381 cccagtcgcg atggccgata tcacaaatat tgctgccctc taccatcagg gtgatgtagt
    13441 ggctgatctt ggcgcggatc tgttccagta aaccttcata agtcagcgca gcgttgcctt
    13501 cattttcatg caggcagatt tccatcactt tcagcaggtt aaacatcgcg atgtcatgca
    13561 agccgtaggt tctgccggga atagataatt ccacgcagcc cactacggaa tagtcgcgcg
    13621 catcttccag cgaaacgcca cggttgagga acgctggcac caccacttca tcgttaaaga
    13681 tttgcggaat accggtaccg aggcgaatag tttctgcggt tttcatcagg aacggcgtgt
    13741 cgatcaaggc gttagtgcgc acccccaggt tcggttgcgg taattgcaca ctttgatagg
    13801 cgtcgagaca gaggaacgaa agtacgttca ccgcgctacg tccgttctcg gttaacccgc
    13861 cgagcagtgc ggtatagccg gtcgggaaac cggcgaaata acgcgcgcta ctggtggagc
    13921 gcaacagcac gatgtcgttg catttcaccc ataaagattc gagcagttct ttcaggaacg
    13981 ccggatcttc gccctgggtt aatgatgcct gatagaacgg caacatatac tggtcgaagc
    14041 gccctaaaga cagagaactg gcgttggatt cgtattgcag aatgatgttc atgtaccaga
    14101 ataactggca cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg cgggagatct
    14161 ccgcaatagt cagcagttct tcgcgacgct gggcatctgt gcagtttgcc gccatcgttt
    14221 ccgccagttc agcgtaacgc agaatgtgtt tccgcgaggc ttccagcagt aacagcgctg
    14281 cctgataaaa gtgattctcc ggctgttgct gacaatgttg ctgcatctgt gccaccagct
    14341 cacccagccc gtgattcagc aggcgcgggt aatcaataat aatgtgcccc tggcctttat
    14401 ccgtctggtt gatgctgaaa atctgcgtgc tggtcgcggc ttttacttca tccgtcatct
    14461 gcccgttaat gaaatctttc atcgaacgtt tttcccagta cgggaacaac tcttcgcggt
    14521 agatacgttt atcttcttcg ctgatagcaa agcggtcctg cggacgcgtc gggaattgat
    14581 ccagctcttt cagcagccag taagggtcca tttccggcga cataattccg gcgcgcggtt
    14641 tcaccgtacg gttaccggca atcagttctt catcacgaat cgaaatttca acatgttcaa
    14701 ggatatgcgc tgttgctttc gcccggcgca atatcaccgg ttcgccttcg gtttgccgat
    14761 ggctggcggt ataaagcagc gcccgctcca gcgagatttc acgggtattg gcaaacagtg
    14821 cagttttgag gcgagagata cgattcgtca tgaggaactc ctgtgggaaa gcgaacccgc
    14881 ccttacgggc gggataagaa cttaagcggt ttgtgccagg tgagcttcaa ttttgctcat
    14941 aatggcatca gcgcgtttta ccgcgtcgct gatgttgacg cgcacgatgg tcttgccagc
    15001 aaaacgctct tcaaacttga tgccgatatc tttggtcaga atcaccatat cggcgctcgc
    15061 cacgtcttcc gcagtcagtt cattttccag accaatcgag ccctgggttt ctactttcac
    15121 ttcccagcct ttcgctttcg cggcactttc cagcgcctct gccgccatat aggtatgggc
    15181 aacaccagat ggacacgcgg ttactgcaat aattttcgtc atggctcgtt cctcaattaa
    15241 ttaatttcaa aatccaaatc caggtcgtct tctttttcat cagtcgaaga cccattttta
    15301 cgcgccagac ttttcagcac gttcacacaa acagccgtca ccactgctcc gacagctact
    15361 gctgcgatat aacccagctt gccttcaacc accggcagca caatcagacc gccccagcct
    15421 gcatagcact gcgcaccgac cagcgccgca gttactgcac cacaaactga accgaccatg
    15481 atggaaggaa taacacgcag cggatcggca gcggcgaaag gaatcgcccc ttcagtaacc
    15541 ccaacgcacc ccatcaccaa cgccgccttg ccggtttcgc gctcttctgc ggagaaattt
    15601 ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg ggatacaaat acccacggca
    15661 gcaatggcca ccacggtata tacaccctga gcaacgcaaa tcagcatgaa ggcataggcc
    15721 actttgttaa ccggaccgcc catatcgaac gccagcatca gacccatgat caccgccagc
    15781 ataacaatgc tgccctgctg catcccctga agccactgag tcaggctgtt ggtcaatgcc
    15841 cccacaggct cgcccagccc ccacatcatg atgcctgcgg taatcagtgt gccgacgatc
    15901 ggaatgatga agattggcat caccgagcgc agaactttat gcaccggaat tttcttcagg
    15961 taatgcacca cgatgccgcc aataatcccg gcgatcagcg caccaaagaa gcccgcacca
    16021 aagctgttgc cgacccaggc accgatagcg cacggggcca gcgcagagcg ttctgcaatg
    16081 gaataaccaa tgtaagcagc gaggaaaggc accatcagcg tcaaacctgc aacgccgata
    16141 tcaaacagtt ttttcagatt cggatcggct acggcatccg gcactgcgcc tttgccatac
    16201 aacatgacgg aaaccgccag caaaataccg cccgatacca cgaagggaat catgtgtgaa
    16261 acgcccgtca ttaagtgctg acgggtattt ttcaggatct gcaccaactc attcataagc
    16321 tgctcccggg cttgcaaaag cccatgtcca tatttttgat ggaggaaaca ataggcaatc
    16381 gcaggcggag caaacagtgg ataaaaaggc catttactgg acttttgtaa tatcagtaca
    16441 aaaatgcgat ccgcctcata acttgcggca acccggaagc atgccgcaaa acggcgtctt
    16501 gtgagacgcg tcgcaaattg acggttacat tacatttgtc cagcttttgg cagatttgtc
    16561 acatggcagg ccgttgccgt gcgctcttat cctgtaaaca gaaaaccgta ccgggagaga
    16621 ggcaatggcc ctgattgtgg aatttatttg tgagctacct aacggcgtac atgcgcgccc
    16681 ggcaagccac gttgaaacgc tgtgtaatac tttttcatca caaattgagt ggcataacct
    16741 gcgcactgac cgcaagggca acgccaaaag cgcccttgcg ctgattggca ccgatacgct
    16801 ggtgggcgat aactgccagt tactgatttc cggggccgac gaacaggaag cgcaccagcg
    16861 tttaagccaa tggctgcgcg atgaattccc ccactgcgac gcaccgctgg cggaagtaaa
    16921 atctgacgaa ctggaaccac tgccggtttc actgaccaat ctgaatccgc gaattatccg
    16981 cgcccgcacc gtgtgcagcg gtagcgcagg cggcattctg acgccgatct cttctttaga
    17041 tctcaatgcg ctgggtaatc ttcccgctgc caaaggcgtt gatgccgaac aatccgcact
    17101 ggaaaacggc ctgacgctgg tactgaaaaa catagagttt cgtctgctgg atagcgacgg
    17161 tgctaccagc gcgattctgg aagcgcaccg atccctggct ggcgatactt ccctgcgcga
    17221 acatttactg gcaggcgtca gcgagggatt aagctgtgcc gaagcgattg tcgccagtgc
    17281 acaccacttc tgcgaagagt tcgcccgttc cagcagcagc tacctgcaag aacgcgccct
    17341 ggacgtacgc gacgtctgct tccagttact ccagcaaatc tacggtgagc aacgcttccc
    17401 ggcaccgggc aaactgacgc agcccgccat ttgtatggct gatgaactaa cccccagcca
    17461 gttcctcgaa ctggataaaa atcacctcaa aggattgttg ctcaaaagcg gcggcaccac
    17521 ctcacatacc gtaattcttg cccgttcgtt caacattccg acgctggttg gtgtggatat
    17581 tgatgccctt accccgtggc aacatcaaac gatttatatc gacggcaacg ccggagcgat
    17641 tgtggttgag ccaggggaag ccgtagcccg ttattatcag caagaagccc gcgtacagga
    17701 tgccctgcgt gagcaacagc gtgtctggct gacccaacaa gcccgtaccg ctgatggtat
    17761 ccgcattgaa attgctgcta acatcgctca ctccgtggaa gcgcaggccg cattcggcaa
    17821 tggtgcggaa ggagttggtt tgttccgcac tgaaatgctc tatatggatc gcaccagcgc
    17881 accgggcgaa agcgagctgt acaacatttt ttgtcaggcg ctggagtccg ccaacggacg
    17941 cagcattatt gtgcgcacta tggatattgg cggtgacaaa cccgttgatt atctgaacat
    18001 tcccgcagag gcaaacccgt tcctcggtta tcgcgccgtg cgtatttatg aagagtacgc
    18061 gtcgttattt accacacaac tacggtcgat tctccgtgcc tccgctcacg gcagcctgaa
    18121 aatcatgatc ccgatgatct cttcaatgga agagatctta tgggtgaaag aaaaactggc
    18181 ggaagccaaa cagcaactgc gcaatgaaca tattccgttt gatgagaaga tccagcttgg
    18241 catcatgctt gaagtgccgt cggtgatgtt catcatcgat caatgctgtg aagagattga
    18301 tttctttagt attggtagta atgacctgac gcaatatctg ctggcagtag atcgcgataa
    18361 cgctaaggtt actcgtcact acaacagcct gaatccggca ttcttacggg cgctcgatta
    18421 cgccgtgcag gcggtgcatc gccagggcaa atggattggt ctgtgcggtg aactgggagc
    18481 gaaaggttcc gtgctgccgt tgctggtcgg tttagggctg gatgagctga gcatgagcgc
    18541 accatcaatt ccggcggcga aagcgcggat ggcgcaactt gatagccgcg aatgccgcca
    18601 gttgctcaac caggcaatgg cctgccgtac ttcgctggaa gtggaacacc tgctggcgca
    18661 attccgcatg acccaacagg acgcgccgct ggtcaccgcc gagtgcatca cgctggaaag
    18721 cgactggcgc agcaaagaag aagtgctgaa aggcatgacc gataacctgc tactggcggg
    18781 ccgctgccgc tatccgcgta aactggaagc cgacttgtgg gcacgcgagg ccgttttctc
    18841 taccggtctg ggctttagtt ttgccattcc ccacagcaaa tcggaacata ttgagcaatc
    18901 caccatcagc gtagcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg aagcacaatt
    18961 catcattatg ttaaccctga acaaacatgc tgcgggcgat cagcacatgc gcattttctc
    19021 gcgcctcgct cgccgcatca tgcacgaaga attccgtaac gcgctggtta acgccgcctc
    19081 tgccgacgct atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg aaacatcatg
    19141 gaactgtatc tggacaccgc taacgtcgca gaagtcgaac gtctggcacg tatattcccg
    19201 attgccgggg tgacaactaa cccgagcatt atcgctgcca gcaaggagtc catctgggaa
    19261 gtgctgccgc gcctgcaaaa agcgatcggt gatgagggca ttctgtttgc tcagaccatg
    19321 agccgtgatg cgcagggaat ggtggaagaa gcgaagcgcc tgcgcgacac cattccgggc
    19381 attgtggtta aaatcccggt gacttccgaa ggtctggcag caattaaaat actgaaaaaa
    19441 gagggtatta ctatacttgg caccgctgta tatagcgccg cacaagggtt attagccgca
    19501 ctggcagggg caaaatacgt tgctccgtat gttaaccgcg tagatgccca gggcggagac
    19561 ggcattcgta cggttcagga gctgcaagcg ctgttagaaa tgcacgcacc agaaagcatg
    19621 gtgctggcgg ccagctttaa aacgccgcgt caggcgctgg actgtttact ggcaggatgt
    19681 gaatccatca ccctgccctt agatgtagcg caacaaatgc tcaacacccc tgcggtagag
    19741 tcagctatag agaagttcga acacgactgg aatgccgcat ttggcactac tcatctctaa
    19801 aggagcaatt atggaccgca ttattcaatc accgggtaaa tacatccagg gcgctgatgt
    19861 gattaatcgt ctgggcgaat acctgaagcc gctggcagaa cgctggttag tggtgggtga
    19921 caaatttgtt ttaggttttg ctcaatccac tgtcgagaaa agctttaaag atgctggact
    19981 ggtagtagaa attgcgccgt ttggcggtga atgttcgcaa aatgagatcg accgtctgcg
    20041 tggcatcgcg gagactgcgc agtgtggcgc aattctcggt atcggtggcg gaaaaactct
    20101 cgatactgcc aaagcactgg cacatttcat gggtgttccg gtagcgatcg caccgactat
    20161 cgcctctacc gacgcaccgt gcagcgcatt gtctgttatc tacaccgatg atggtgaatt
    20221 tgaccgctat ctgatgttgc caaataaccc gaatatggtc attgtcgaca ccaaaatcgt
    20281 cgctggcgca cctgcacgtc tgttagcggc gggtatcggc gatgcgctgg caacctggtt
    20341 tgaagcacgc gcctgttccc gtagcggcgc gaccactatg gcgggcggca agtgtaccca
    20401 ggcggcgctg gcactggcag aactgtgcta caacactctg ctggaagaag gcgaaaaagc
    20461 gatgcttgcc gccgaacagc atgtagtgac tccggcgctg gagcgcgtga ttgaagcgaa
    20521 cacctatttg agcggcgttg gttttgaaag cggcgggctg gcggcggctc acgcggtgca
    20581 taacggcctg accgctatcc cggacgcgca tcactattac cacggtgaaa aagtggcatt
    20641 tggtacgctg acgcagctgg ttctggaaaa tgcgccggtg gaggaaatcg aaaccgtagc
    20701 tgcacttagc catgcggtag gtttgccaat aactctcgct caactggata ttaaagaaga
    20761 tgtcccggcg aaaatgcgaa ttgtggcaga agcggcatgt gcagaaggtg aaaccatcca
    20821 caacatgcct ggcggcgcga cgccagatca ggtttacgct gcactgctgg tagccgacca
    20881 gtacggtcag cgtttcctgc aagagtggga ataacctact ccaaactccc ggattgtccg
    20941 ggagtttgag agtgcaggca aagtggctca ctcgtcactg cgggcatgtt tttccccctc
    21001 gccctttcag ggagagggtc gggatgaggg taaatattcg ccccggagct ggcctgttcc
    21061 cctcacccta accctctccc caaaggggag aggggactgt tcgagcactt tttatacgtt
    21121 atcacccgtc tgaaactcac acgttgttca ggaatttttt atccgcttct ggacattttc
    21181 tctacagaac aatcgttagc ccggaaggtc gaatctgtga ctcttatttt cacggacgaa
    21241 ccagatgaaa gcttcactgg cgctggtcag cctgctaacg gcgtttacca gcaactcctt
    21301 aaaatctccg gccgttccac caaccgtggt acaaatccag gccaacacca accttgccat
    21361 cgcagacggt gccagacagc aaatcggcag cacgctattt tacgatccgg cgtatgtgca
    21421 gcttacctat cctggcggtg atgtcccgca agaacgcggc gtatgttccg atgtggtgat
    21481 ccgcgccttg cgcagccaga aggtcgattt gcagaaactg gttcacgaag atatggcgaa
    21541 gaattttgct gagtacccgc aaaaatggca gttaaagcgc ccggacagca acatcgatca
    21601 ccgtcgggtg cctaatctgg aaacctggtt tagccgccat gataaaacac gccccaccag
    21661 caagaacccc agtgactatc aagcgggcga tattgtctcc tggcgactgg ataacgggct
    21721 ggcgcatatt ggggtggtgt cagatggctt tgcccgcgac ggtacgccgc tggtgataca
    21781 taacattggc gcaggcgcgc aggaagaaga cgtgctgttt agctggcgga tggtggggca
    21841 ctatcggtat tttgttaaat aagcgatcgc cccacatgaa gaaaaaattc agatgttttt
    21901 tacactgaca gcttcttttt tcttacggct caccagcgcc agtgccagca caatcagcac
    21961 aataccgcta ccttcaacgg cccccggatt ctcaccgagc aaccaccagg aaaagagtac
    22021 gccgcaaacg ggaacggcca gtgtgcttaa gctggcaata ctggcaggca agtttttcaa
    22081 tacaaacaac cataagctcc acgccagcgc cgtcgccaga atcgcactgt aggccagagc
    22141 ccagaacacg gtgggctgcc agtcaatttc acgttgtggc accagtaaag cgaccgcact
    22201 catcaccagc gccgcataaa gcatctgcca ggatgttaac gacaataaat ccacgcgtgg
    22261 atggcgggcg tataggcgtt tcgcgacaat cgcgctcgcc ccccaactaa caccggagag
    22321 gattgccagc agggcacttt tcatcgaaga gaaatcgagt tgccacggct gcaacaccaa
    22381 aaataagccg aaagcggcaa tcagtatcgc gaaatattgc ccacgtcgca ggcgttcacc
    22441 gagaaacaac gcggcgaaaa tcaccaccca gaacggcatg gtatagctca ggatcgccac
    22501 cttccccgct cctccgctaa ccagcgccca ctgcgccagg ccaaccatcc cacaggtttg
    22561 taacagggca atggctaagg tgtatttaaa cggtgtcggg cgcattccgc gaccacgtaa
    22621 taaaaggaca atgaataaaa cgagagcgcc gaaaatgcag cgtaaggcgg tgaagtcgaa
    22681 ggcaccgatg tagctggtga cttgcttcat gaaaatccag ctataactcc agataagtgt
    22741 gagcacaacc aggccactga ttgccagtgg gttgctcttt cctgcggcag acatgagaga
    22801 tccagtgtgt agttttcctg tggacactat acgccggtaa agaaacttca gcgactgcca
    22861 tcaggcagtc gctgaagaag gtcagattac agcaggtcga aacggtcgag gttcatcact
    22921 ttcacccatg ccgccacgaa gtctttaaca aacttctcgt gggcatcgct gctggcgtaa
    22981 acttccgcca ccgcacgcag gacagagtta gaaccaaaga ccagatccgc acggctggca
    23041 gtgtatttca cttcgccggt ttcgcggtca cggccttcga acagctcttt cgattcgtcg
    23101 gtcgctttcc actcgtaacg catatccagc aagttcacga agaagtcatt actcaataca
    23161 ccaacgcggt cagtgaagac gccgtttttg ctgccatcga agttggcacc cagtacacgc
    23221 atcccgccca ccagcgcggt catttccggc gcggtcagcg tcagttgctg tgctttatca
    23281 atcagcagtg actcggtggt agaaacatcc agacgagcgc ggtagttacg gaaaccgtca
    23341 gcaattggct ccagcagttc gaacatttca atgtcagtct gatcctgacg cgcatcaacg
    23401 cgacccggcg caaacggtac atgaatgctc aaacctgcgg cgcttgcggc tttctcaacg
    23461 ccaaccacac ctgccagcac gatgatatcc gccagcgaag ctttgccaga ctctttctga
    23521 attttctcca gaactggcag agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc
    23581 ggcattaatg ccagacgtgc accattggca ccaccacgtt tgtcgccacc acggaaggta
    23641 gaagcagacg cccaggccac cgataccagt tcgctgacag acagaccaga atccgcaatc
    23701 gcgaatttca gatcgataat gtcctgctcg gtcgggttgt agatcggctg cggcagcgga
    23761 tcttgccaga tcagatcttc tttcggtact tccggcccga tgtagcgaga tttcggcccc
    23821 atatccctgt gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga
    23881 tcgttgagga aacgacgaga aatcttctcg aactcaggat caaaacgcag cgtcaggtcg
    23941 gtcaccagca ttgtcggttt acgtttcttc gacggatcga acggatccgg gataatttcc
    24001 ggtgcgtcta ccgcttcgaa ctggattgcg ccagccggac tgcgggtctg tacccactca
    24061 tacttgaaca ggttctcgaa gaaatagttg ctccactggg tcggcgtctg ggtccagact
    24121 acttccagac cagaggtaat ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag
    24181 cctaaacctt gttcttcaat cggtgcagct tctggatcag gacctacatt tgatgtcgga
    24241 ccggcaccgt gggttttacc cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg
    24301 tcgttcatgc ccatgttgcc gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg
    24361 ccgctgtgat ccgggccttc cgggttaacg taaatcaggc ccatctcggt tgcgcccagc
    24421 ggtgctttcg ccagtgcttc cggatgacgg tgggtcagcc aggctttttc atcaccccag
    24481 ttaacatcca gatccggttc ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg
    24541 aagccggagt tttccagcgc cacgttaccc gcgaggataa acaggtcggc ccaggagatt
    24601 ttctgaccat atttctgttt gattggccac aacaggcgac gcgctttatc gaggcttacg
    24661 ttatccggcc aggagttcag cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt
    24721 ccgtcgatgg aacggtaagt acccgcgccg tgccaggcca tacgaataaa cagaccggcg
    24781 tagctgcccc agtcggcagg ccaccacggc tgagattctg tcagcagggc tttcagatct
    24841 tttttcagac cgtagtaatc taatttgctg aattctttgc ggtagtcaaa gtcctcaccc
    24901 agcgggttag aacgattaga atgttggttt aacaggtcaa cacggagttg atttggccac
    24961 cagtcgcgcg tggttgtgcc cccacctgcg ctctggtcgt gaccgccctg atggaacggg
    25021 catttgccgg tggctgtcgt gttatggata tcgtctgacg tgctcatcaa tgtgctcccc
    25081 tctacagtgt taccgttacg atacacagcg ttagagagaa gttataattg aattaatcca
    25141 cggatgcgat agttgagatc ttacatattg ttggttaaag agatgtagat caaattgatc
    25201 gtaattaaga ctttttgatt atgtccgaat tcggacaaat gctttataaa aaagggtttt
    25261 agcttcatat ccttcaagta atgagggaga aaacaaggct gacaacctta ttttcccgcc
    25321 ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt gtgattttca
    25381 caaaagccac actatttata aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact
    25441 catttcagca cggttaagcg tatagaagtg gaaatctttc actccttcac ggcttaaaat
    25501 cttcaccata tccatcgcaa tattcgcgcc caccagtttg cgggtttcgg catcgtcatc
    25561 cagcccgtca aacatttgcg ccatccacgc cggaatacgc acgttggtca tgtcggcaaa
    25621 tttcttcgct tgtttaaagt tcgagaccgg caaaattccc ggaatgattt ccacatcaat
    25681 gcccgccgat acgcagcggt cacgaaaacg caggtagctt tcgacatcaa agaagaactg
    25741 agtaatcgcg cggttggctc cggcatccac tttgcgtttc agattaagca aatccgcctg
    25801 agcgcttttt gcttccgggt gaacttccgg atacgccgcc acggatatat cgaaatctgc
    25861 cacttctttt aacagcgtca ccaggtcaga agcatacatt tctggcttac cgcttcccgg
    25921 cggcagatcg ccacgcagcg ccacgatatg acgaataccg ttattccagt agtcacgtgc
    25981 aatggtgcgc agctcgtcgg gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag
    26041 accagtgcga tctttaatgc ctttaataat gctgtgcgta cggtcgcgct cgccggagtt
    26101 cgcgccatag gtcaccgata caaacttcgg tttcaggctg ctaaggcgat cgatggagtt
    26161 ccacagggtc tgctccattt cactggtgcg cggcgggaaa aactcgaacg aaacgttaat
    26221 ctgcccctgg acttctgcca gactctgatt cagggcatcc cgctggctgg cgtgaaaaaa
    26281 gctcatacct tacctcatca atcgcttgta gttatatgtt gtgttgtgaa catctatcca
    26341 tttagacgtc cagatgtaaa gatgaaggaa aagccgaggg gcgtcaaccg aaaaatgacc
    26401 aatgatggtg aggaaaaatc agggaagttg aaaaaaactc atcttaatta gaggcgataa
    26461 aagatttctc tatagcgcaa tccacctcgc acgttttgac tgatattctg acaacacagc
    26521 gacacctggg cttattcgaa aaacaacgtc ctgccaccgg atggtgatgc taaggcatct
    26581 gattcggcct gcgccctcgc gtaggccgaa tactggatct gcaaaatcaa ctggtaacaa
    26641 acagatgaat caactcaata cgtactttta cttgatatct ttcacgcgca taccgttgat
    26701 ctgttcatcc gtgatggtgt tacccgcttt gaagtaatca accacggcgt gataaacgta
    26761 ataaccgccc gttgtgttac gcgctttccc ttcggtaaag gcggtaaaac catctccacc
    26821 atcagcaagg aaactctgag tagcaatgtg gtaaatcgtc gcatcttcaa tgggtttgcc
    26881 attcagagtc agcgtgatta cccgctgacc aaccggctta ctgctgtcgt acttcatttc
    26941 caggcctttc gatacctgta aaacgccatt acttaaactt gcgccgtgtt ccatcaaact
    27001 gcgtaattgt ttacccgtga gatccatcgt caccagttcg ttgggaaacg ggaaggtact
    27061 gatgacgccg cccatcgtaa tcgcccctgc ggggatctca ttgcgaatgc cgccagagtt
    27121 ggttaacgcc aattgggtgt ttttacccgc cgctaccagt aaagcatcag ccgccaggtt
    27181 cccgagggaa gccgattcac cataggcacg ttttaattca accggcgatt tcgcgaccgt
    27241 ttgttgcacc acttcatcaa gctttttatt ccagccgtcg atcacctgtt ttgtttgcgg
    27301 atcgggcttc cactcgtcgg cgtaaatggt tttaagctcg aagtttttca ccgtaaaatc
    27361 atgcggcttc tctttgtagt cgagaaccag tttgcccaca tcaatcccgc cgctgtcagt
    27421 ggagaggatg agcgtattgc cgactttaat cggttccggc gtacccacat gcgcatgtcc
    27481 ggtgatcaaa atatccagcc ctttcacctg acttgccgtc tgaatatctt tatccagcgc
    27541 gcgacgcaca tctgtgcctc ccatactgga ctggcgggcc ggaacacctt catggatcag
    27601 ggcgacggtc aaatcaacct tgcctttaag ttcatcgata taacgttgta gccatttaat
    27661 ttcatcacgc gcttcaatcc ccactcgcgt tgccgcagat accgtgtcat taaaggcaaa
    27721 tacaccgtgc aaacctataa cgcctatttt cacgccgtct ttttcgatga tggtatatgg
    27781 cttatcccag aatgatttac tgctgttctg ataaaaaaca ttgccctgca caatagggaa
    27841 ttttgcctga ctcaactgta ataatgtgtt gtcccagccg tggtcaaatt catggttgcc
    27901 tatcgtgacg gcatcaaacg gcatagtatt cataatatcg ataatagcct tacctttggt
    27961 caggctgctg atatatggcc cggtaaaata atcacccgca tcaaaaaacc atgtcgcttt
    28021 attttttgct ttttcttgct taaccagcgt agtgatattt gcccagccgc caatatcccg
    28081 tttaccatca gcgatccacg gcactttata aggctcgaca tgcgcatgga ggtcgttggt
    28141 ataaatgatg gtgacatctt tggcgcaagc ccagaacggc aatgttaatg cgatacccgc
    28201 agcaagtatt ttaattttca taaatagtcc ctttcagtga tttaaaggat tgctacatta
    28261 cgttctgaaa gagttattta tgtgagagta atcacatccg caggggatag ttgccggaca
    28321 aaatcaacga ccttgtgaaa tcgttggtga tatatgtcga ctctgtttac gattaatgcc
    28381 tgcaagtcgt ttggttgtcg taatttaggg caaccaacat cgacagatta cagctggcca
    28441 gactatcgac tgggttatcc ggcactgcac tgtcgggcat gtggaagtta cccgccgttg
    28501 tttgacgaac agcaatttcg tgactggttg tcagttcact tatctacttt tgcaactgag
    28561 aaaggtcatt tctgcccggt atgttatggc acaaagacga tttgttatgg ccacaatcca
    28621 caggggtcgc aacgcattca atgtcgtaac tgcaaaaaag tctggacacc taaacaatat
    28681 cagaaagaaa taacgcctcc tgaaataatc gaaacggttg ctcttctggt gccatttcag
    28741 ggagccagcg gcgggcagaa actgtatgtt ttaatcagtt ttgatgctct tcgcggtaat
    28801 attcttcatc tttctactaa ttacacgcag catcaggctg gagaaagtct gcattatcga
    28861 tacagaggga atgcagaacc agagttacac gagagtaata ttgtacaacg cgttgatatg
    28921 cgcgaagcac aatttctacg ccgcagtcag tttgatgaga ttcaatatgg cagcgcagcg
    28981 ctcaaacgca atgctaaagg tgttattctg cgtccggtta tcactgcaca cgggcatttc
    29041 agagtactga atattttatt ccctacagta aaaacacatg taatttcaca cgagtgtttt
    29101 ttaagaggtg ccattattac ggcatgggct gatctgtttc gccaacagca aggtgagatt
    29161 tggtttattg aagaggaaat agctgacgat accgataata tgccctggcg ttttcaggga
    29221 acaacttatc acggctggtg gaaaaatcag tggcaactct gggttcaggg caaaaatcgt
    29281 aaaatggtat gtgcattaac gggcggaaat aacagcaaag cagaaatgct ctctttagca
    29341 accagcagac attttatcga ctggctacat aaacaagcag tatttactca cagcgccccg
    29401 ctttccgcag ggcgcgttac gcaaatactg ctttctctgg ctcaggatta caacaactgc
    29461 gccaggcggt taatatccga ctgaatcgcc cctgcggtga cgtcgcgccc cgcgccaggc
    29521 ccacggatca ccagagggtt atcgcgatac cagcggcttt cgatggcaaa gacgttatcg
    29581 cacggcagca gtgatgccag cggatggtct tcacgcaccg cttccacgcc cacacgcgct
    29641 ttgccattgg catcgaaacg cgccacatag cgcagcacca gccccatttc gcgggccgct
    29701 tccagtcgtt gcaccatctg ctcgttaagt tcatcgccgt tttcaaagaa atggtcgatg
    29761 ctaccgcctt cgcaatgggc aggcaccagc gattccacgc gcacctgatc tggttcgatg
    29821 tcgtaacccg cttcacgcgc cagaatcacc agcttgcgca tcacgtcttt gccggaaagg
    29881 tcatcacgcg gatcaggctc ggttaagccc tgctgccacg cctgatccac cagctcggta
    29941 aacggcacgc taccgtcgaa ttgcaggaac agccaggaga gcgtgccgga gaagatccca
    30001 ctgatcgaca aaatagtatc gccgctgtcg atcagatcgc gcacggtatg gttgatcggc
    30061 aatcccgcac cgacggtggc gttgtacagc cagtgacgcc cggttttttc gaaggcgtcg
    30121 tggatctggc gatatttatt gctgtcgctg gctcccgcca gtttgttggc gctgatgacg
    30181 tggaaaccgt ggctggcaaa atcgagatac tgatcagcaa gctgctggct ggcggtaacg
    30241 tccaacacca ctaaatcatc atacggatgg gcgcgcatcc acaggaacag cgactcttca
    30301 tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc ggctggcgtc cagcccgtca
    30361 tagctcaaca ggctgcggcg gctgtccacc acgcctgcca gcacaaattc aaagccggta
    30421 cgcgccgaaa gcgtgctctg ctcacgggcg aacagttcca gccagcggga accgatattg
    30481 cccttgccga acaataccag gccgatgcgt ttttctgcgc ggaagacgga ctgatgcagc
    30541 ccctgaatca ggctttcggt cggtccggtg cgcagtactg ccaccaggct gatgccgtca
    30601 tcggactgcc aggtaaactc gaccggttgg cctttcagtt gctgccagaa gcggtgacaa
    30661 tgcagcgggt tacgggtgac gcccgccccg accatcgcca ccagcgccag cccctggcgc
    30721 agacgcagtt cgccaggtaa tcccgcttcg tcgaggattt tcagcgcact gtcggccact
    30781 tctgaggtgt agcaaaattg cagcaactgg cgatcgttat gcacgccaac tgccagtgga
    30841 cgcacctgcg cgcgtttcag gatctggtcg atctctttat gcgccagttt gaaatcctgg
    30901 ctggcgggca cctgaaactc aatcaaacag acatcatcgt ggctggtgac aatacgcgca
    30961 ccagtaccgg aggccagcac gcgttcaatg cgcgtggagc cttgatccgg cgtgtagcta
    31021 cagcgcagct gcaggtcgat ttcgctgcca gaaactggct gtaaagtacg ggcgtgaaga
    31081 acgggagccg ccagacgcgc cagttcgcta gcttcatcca gacgcagcaa tggcagcaga
    31141 caggcatctt tcactttacg cggatcggca ctgtataccc cggcgacgtc gctccagatg
    31201 gttacacgag aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc ggaaccgtta
    31261 cgccccagca gcaccgtttc accagcgttg ttacggctga taaacccggt caccaccaga
    31321 cgtttacccg gatgttgtac cagtagctgt tgcagcaacg ggtaagaaag cccttcatca
    31381 acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcgc gggcatcaag ccaggccgct
    31441 ggcagccctt gttgattaag taccgcagac atcaggcgcg ccgaccacac ttcgccgtgg
    31501 cccaccactt ccgcatacac cgcgtcgtta ataccgctgt cgagcagcgc cgccaggcgt
    31561 tccagatcgc tgacaaaagc gctaatgagg ctatcggctt cttcggcggg taacaggccg
    31621 ctaatcagat cgcactgata gcgacgcagc gtttgttgaa cctgatgcgc agagagacga
    31681 tcggtctggc ttagtttcaa ccagttaatc aactggttag tggtgctacc ggcggcggaa
    31741 accaccatca tatcgtcagg ctgagagtac tccgccataa tgcccgcgac acgcaaataa
    31801 cacttcacat cagccagact actgccacca aatttatgca gttgacgacc tttcgcccct
    31861 gcctgcgcaa tcacactcat ttttacccct tgtttgcagc ccggaagcca ttttccaggt
    31921 cggcaattaa atcttcgcca tcttcaatac cggtggagat acgcagcagc gtctcggaga
    31981 tcccggcggc ggcacgcgct tctggtgcca tgcctgcatg tgtcatggtt gcggcgtgag
    32041 agattaaact ttccactccc cctaatgatt ccgccagcgt aaacaacgac agcccgccca
    32101 ggaaacgacg cagcgtctgc tcatcgccat ccagttcaaa actcaacatt gcgccaaagc
    32161 ctttttgctg gcgcgcggca atttcatgcc cctgattttc cggcagcgac gggtgataca
    32221 gttttttcac caacggctgg gtttgcagat atttcacaat cgcctgcgcg ttgcgctggg
    32281 ccagctccat acgcggcacc agcgttcgca aaccacgtag cagcagatag ctgtcaaacg
    32341 cgccgcccgt cacgccaata ttgtttgccc accaggccag ttcggtgaca acgtccggat
    32401 ctttagcaat caccacgccg gccaccacgt ctgagtgacc gttcagatat ttcgtgcatg
    32461 aatgcaacac cagatcggca cctaatgcca gcggattttg taatgccggg cttaagaagg
    32521 tgttatccac cacgctcacc gccccgactt cccttgccag atggcagatt ttcgcaatat
    32581 ccacgacgcg taacaatgga ttacttgggc tttctaccag taccagtttg gggttttctg
    32641 ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg cgatagcaac
    32701 cgcgtttcgc caggctgtcg aacaggcgat agctgcctcc gtagcagtcg tgcggcgcaa
    32761 ccagcagatc gccaggtttc aaaaagacgg tcgttaccag gtgaatcgcg gacatgccgg
    32821 tattagtaag tactgcacca gcaccgcctt ccagttctgc cagcgcacgc tgaaccacat
    32881 cgcgcgttgg gttgccgcgc cgcgagtaat catgcgcgcg cggttcatta aatccggtaa
    32941 agttataggt gctggaaaga tggatgggag ggacaacgca accatactgt tcgtcgtcat
    33001 ttaacccgct acgcactgcg atggtggcct gtttacgcgt catgtgatga agttccctgg
    33061 gctttgtcgg tgaaatgtca ggcaccagag taaacattgt gttaatggac gtcaatacat
    33121 ctggacatct aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc gttaaaatta
    33181 tatgcattat cacgccgaca ggtgcattac acgatgtcac ggtagcgcct gtacggtaaa
    33241 ctatgcgggt ttacggtcag tacccacata aattgtgtgg tctggtctca atttattgac
    33301 gaagaggatt aagtatctca tggctgaatg gagcggcgaa tatatcagcc catacgctga
    33361 gcacggcaag aagagtgaac aagtcaaaaa gattacggtt tccattcctc ttaaggtgtt
    33421 aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc acgctaccaa
    33481 cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt tgccggatga
    33541 tgccgatctg cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag agatcatgcg
    33601 tgagatgggg attaacccgg agacgtggga atactaaagc ggaaaagagc ggcgcggagt
    33661 taaccagccg cttttttcat acatggacgt ttaacgatga aaatcaggct actgatcctc
    33721 agcctgctgg tttctgtgcc tgcatttgcc tggcaaccac aaaccggcga catcatcttt
    33781 cagatctctc gctcatcgca aagtaaagcg atccaactgg cgacccattc cgattacagc
    33841 cacaccggta tgctggtgat acgcaacaaa aagccctacg tttttgaagc ggtcggcccg
    33901 gtgaaataca ccccgctcaa gcagtggatt gcccatggtg aaaagggcaa atacgttgtt
    33961 cgccgcgttg aaggcggact gagtgttaaa caacagcaaa aactggcgca aacggcaaaa
    34021 cgttatctcg gcaaaccgta tgatttcagc ttctcgtgga gcgacgatcg ccagtactgt
    34081 tcggaagtgg tgtggaaggt ttatcagaat gcgctgggaa tgcgcgtggg cgaacagcag
    34141 aagttgaaag agttcgacct gagcagccca caagttcagg caaagctcaa agaacgttac
    34201 ggcaagaata ttccgcttga ggaaacggtg gtctcgccac aagccgtttt cgatgcgcca
    34261 caactcacca ctgttgccaa agaatggccg ctgttttcgt ggtaatggat gataaaaaaa
    34321 ataattcccg ccgtagtctg tttgttgctt tgtagttgca cgacctttcc gcaagctgtc
    34381 gctccggtaa acggtgatgc ctccaattag ttgaattaag gatataatac tcgcttttaa
    34441 ggttgtttca tggtcacagt taaccttcat tgtccccgtt gccagtccgt tcaggtttac
    34501 cgtcacgggc agaaccccaa aagacatgat cgattccgcg gccgtgactg tcaccgggta
    34561 tttcagctca gttactgtta cgagccccgt aaaccaggag ttaaagacca aatcgctgaa
    34621 atggctttca acggtgccgg ggttcgctat actgcccgta cgctgaaaat cggcattaat
    34681 acagtcatcc gaactttaaa aaactctcgc cacgaaaaat aacgtcatcc ccggtcgctc
    34741 atgctgatgt ggcactcatc tgcgaactta gtcagcaatg gagctttgtc ggcagtaaag
    34801 cgcgacagca ctggctctgg tacgtgtaca acacgaaaac tggtggtgtt ctggcctaca
    34861 cttttggccc ccggactgat gaaacctgtc gtgaactgct ggcgctgctt acccttttac
    34921 catcggcatg ttgaccactg acgaatgggg tagttacgct agagagttac caaaggagaa
    34981 gcatctgact ggtaagattt ttactcagca cattgagcgt aacaatctga ccctgagaac
    35041 ccgaatcaaa cgtctggccc gcagaaccat ctgtttctcg cgttctgtag aacttcatga
    35101 aaaagtcatc ggtgctttta tcgaaaatat atgttctact aattggagtc atcacccttc
    35161 tgatgagctt tccttggatg attaccagac tgaatacaca gccaaagatt tttacctcat
    35221 tctgctcttt tttctcaaat gacatatatt atatttt
//
LOCUS       DARNVM010000031        34636 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.031, whole genome shotgun sequence.
ACCESSION   DARNVM010000031 DARNVM010000000
VERSION     DARNVM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.031"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..569
                     /locus_tag="SI621_001411"
     CDS             <1..569
                     /locus_tag="SI621_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4064143.1"
                     /translation="KKLGFALSGLMLAMVAGTASADMDGGQINISGLVSDNTCETKVD
                     GGKQDGLVLLQTATVAEITAGVSETAVGAKAKPFSITVDCSKATPGGQPTLTFGSVFF
                     GNSRGTLNNDMSITTPSSGVNIALHNIDGTTVKQVKINNASDAYTKALTNNVAVFDFK
                     ASYVREDDSTPATAGYVRTNTAYTITYN"
     gene            659..1393
                     /locus_tag="SI621_001412"
     CDS             659..1393
                     /locus_tag="SI621_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HEI4064144.1"
                     /translation="MLLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVS
                     VRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGST
                     VLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPL
                     ALKWKWAEGKSGLSVYNPTPYYVSFNNAELIAGGKSNPLNVKMVAPKSEEIFTISGSG
                     GKATSGKVHFSAINDFGGAIEGDVNL"
     gene            1428..4028
                     /locus_tag="SI621_001413"
     CDS             1428..4028
                     /locus_tag="SI621_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HEI4064145.1"
                     /translation="MKTTTSTKRHNLTRIAMYCSIMYSGATIGAESVEYDPTFLMGGN
                     ASSIDVSRYSDGNPTLPGVYDVSIYVNEQPVANLEIPFIAIPDKKNAQACITLKNLLQ
                     LHIKTPPADEENTILLPRDETLGNCLDLSLAIPKSSVNYDPSEQRLDINVPQAWVMKN
                     YQNYVDPSLWENGINAATLSYNINAYRSENSNYTNDSVYTSFNGGVNLGAWRLRSSGN
                     YSWRNDAGSNVEFMNRYVQRDITAIRSQLIMGESYTTGETFDSVSIRGIRLYSDSRML
                     PPVLANFAPTIRGVANTNAKVSITQSGYKIYETTVPPGPFVIDDLSPSGYGSDLIVTI
                     EEADGSKRTFSQPFSSVIQMLRPGVSRWDISGGQINKDDLHHEPNLLQATYYRGLSNL
                     FTGYTGFQVTDNHYAAGLLGIGMNTSVGAISFDVTHSSVDIPDDKRYQGQSYRISWNK
                     FFDLTDTSLNIAAYRYSTQDYLGLNDALTLIDEVEHPTQDLDPKTMRNYGRMKNQFTV
                     SINQPLRFGKDDYGSFYTSGSWSDYWGGSKSRSNYSVGYSNSASWGSYSISAQRSWDE
                     YSQTENSIYLSFSIPIEKLMGTEHRDSGFQSIDTQLSTNMDGNNQFNMSSSGYSNDNR
                     VSYSVNAGYGMNKTGKDLSNIGGYASYESPWGTLAGSVSATSDNNRQYSVNTDGGFVL
                     HSGGLTFSNDSFSDNDTIALVKAPGAKGARINYGNNTVDRWGYGVTSALSPYQENKIA
                     LDTENLENDIEMKSTSTVAVPRQGSVIFAGFETNQGQSAIMNIKRSDGKGIPFAADIH
                     DETNAIIGNVGQGGQAFVRGIQQQGTVKITWLEASMPKTCIAQYQQPNVTSEKIQQTI
                     ILNNILCQLQ"
     gene            4047..4607
                     /locus_tag="SI621_001414"
     CDS             4047..4607
                     /locus_tag="SI621_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4064146.1"
                     /translation="MIKNTRYKIVLLAGIFLLPGALAQDINVDFVATIKGTTCNITLE
                     GTRVTADGNNQYTLRILNVALDKIINKMPEAQADFKLVANCDGNIGKITTSLAGNPSV
                     ELPYLISPLTSDNSSTTEYIAMGIKLRNASDTEFIPPDGSKKISWGEGLTKPELEMTV
                     ALRETRPGSGRAGNFKALATFNFTYE"
     gene            4621..5220
                     /locus_tag="SI621_001415"
     CDS             4621..5220
                     /locus_tag="SI621_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4064147.1"
                     /translation="MQMKSLHYGIFASITLSLLLFSTFSIAITDNIGLNVMTTVENRT
                     CQSEISNQGLVNLGTVGVGYFASNVSAEDNYPGGKTFTVTVKDCPSTGTTSSQLHIEF
                     KPVSGVMATGSQQIFANNHVGGAQNVGIVIFSQNNQSSDTVNVLSPTGQFRTVWPVAS
                     ANMNNSSWQFYTRMQKIDPLLPVGAGFVTSNVLVDIYYQ"
     gene            5272..6522
                     /locus_tag="SI621_001416"
     CDS             5272..6522
                     /locus_tag="SI621_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HEI4064148.1"
                     /translation="MKSAFRYFLFLWIITASINNVNAGNLTFMVGDDAGVGNLNGPSG
                     DKLISFAYPVGGVNNIVFYNPTSATPTNAQIKLYWSELDTSSSRGFVYCTSKSGSSGM
                     PITVRHNMVDSGKMADGHRLYETNVTGLYYTLMLNRFWSYELRSSSPDIYVGDDGWHT
                     VQFSITGDYIRGQCNTSRYQAVGGLLHDATVEFYTDSSFNPAPNTNVQLKHTGDYLYS
                     FANEGPGIPNVSASKMLKINFNLTDINIQLPTCFTSILLGESVTRSTVNLGEYTVGQI
                     TSDSATPVNFQISLQNCVRISSIKTKLKSNVVGVNNPLLLGNTLTGTDDAKGAGVLIE
                     GLPNSKNSQNFTLKPNDETSSYNDAEDDPGENNGIYNPDYPNANGRTTTQDLKFQATL
                     KRDGNATVKPGGFKATSTFQVTYE"
     gene            6636..7430
                     /gene="panB"
                     /locus_tag="SI621_001417"
     CDS             6636..7430
                     /gene="panB"
                     /locus_tag="SI621_001417"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEI4064149.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATQEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7442..8293
                     /gene="panC"
                     /locus_tag="SI621_001418"
     CDS             7442..8293
                     /gene="panC"
                     /locus_tag="SI621_001418"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEI4064150.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELV"
     gene            complement(8659..9603)
                     /locus_tag="SI621_001419"
     CDS             complement(8659..9603)
                     /locus_tag="SI621_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEI4064151.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEGRQEVRQEVRQEVRQEVIQEV
                     TQEVSQEFAQRLLSKGMSREDVAEMANLPLAEIDKLIN"
     gene            9877..10257
                     /gene="panD"
                     /locus_tag="SI621_001420"
     CDS             9877..10257
                     /gene="panD"
                     /locus_tag="SI621_001420"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEI4064152.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10261..11490)
                     /locus_tag="SI621_001421"
     CDS             complement(10261..11490)
                     /locus_tag="SI621_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEI4064153.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVFNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYVTLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11554..11994)
                     /locus_tag="SI621_001422"
     CDS             complement(11554..11994)
                     /locus_tag="SI621_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEI4064154.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWCGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12099..12869)
                     /locus_tag="SI621_001423"
     CDS             complement(12099..12869)
                     /locus_tag="SI621_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4064155.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12866..13792)
                     /locus_tag="SI621_001424"
     CDS             complement(12866..13792)
                     /locus_tag="SI621_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4064156.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13901..14563
                     /gene="can"
                     /locus_tag="SI621_001425"
     CDS             13901..14563
                     /gene="can"
                     /locus_tag="SI621_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEI4064157.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14604..15140)
                     /gene="hpt"
                     /locus_tag="SI621_001426"
     CDS             complement(14604..15140)
                     /gene="hpt"
                     /locus_tag="SI621_001426"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEI4064158.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15346..17736
                     /gene="gcd"
                     /locus_tag="SI621_001427"
     CDS             15346..17736
                     /gene="gcd"
                     /locus_tag="SI621_001427"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HEI4064159.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17783..19333)
                     /gene="cueO"
                     /locus_tag="SI621_001428"
     CDS             complement(17783..19333)
                     /gene="cueO"
                     /locus_tag="SI621_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HEI4064160.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNNPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19499..19846
                     /locus_tag="SI621_001429"
     CDS             19499..19846
                     /locus_tag="SI621_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HEI4064161.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKTLGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19952..20818
                     /gene="speE"
                     /locus_tag="SI621_001430"
     CDS             19952..20818
                     /gene="speE"
                     /locus_tag="SI621_001430"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEI4064162.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDASVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            20834..21628
                     /gene="speD"
                     /locus_tag="SI621_001431"
     CDS             20834..21628
                     /gene="speD"
                     /locus_tag="SI621_001431"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEI4064163.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21666..22028)
                     /locus_tag="SI621_001432"
     CDS             complement(21666..22028)
                     /locus_tag="SI621_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HEI4064164.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22225..24822)
                     /gene="acnB"
                     /locus_tag="SI621_001433"
     CDS             complement(22225..24822)
                     /gene="acnB"
                     /locus_tag="SI621_001433"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEI4064165.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25177..27030
                     /locus_tag="SI621_001434"
     CDS             25177..27030
                     /locus_tag="SI621_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEI4064166.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     LTVSDAAAVTLPATVSAPPVTPAVVKSAFNTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVLTANHVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSDHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(27101..28525)
                     /gene="lpdA"
                     /locus_tag="SI621_001435"
     CDS             complement(27101..28525)
                     /gene="lpdA"
                     /locus_tag="SI621_001435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEI4064167.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28850..30742)
                     /gene="aceF"
                     /locus_tag="SI621_001436"
     CDS             complement(28850..30742)
                     /gene="aceF"
                     /locus_tag="SI621_001436"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEI4064168.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAGPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAVKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAVTGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30757..33420)
                     /gene="aceE"
                     /locus_tag="SI621_001437"
     CDS             complement(30757..33420)
                     /gene="aceE"
                     /locus_tag="SI621_001437"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEI4064169.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33581..34345)
                     /gene="pdhR"
                     /locus_tag="SI621_001438"
     CDS             complement(33581..34345)
                     /gene="pdhR"
                     /locus_tag="SI621_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HEI4064170.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8687 a         9106 c         8324 g         8519 t
ORIGIN      
        1 ctaaaaaatt aggttttgcc ctgtctggtc ttatgctggc aatggttgca ggtactgctt
       61 ctgctgatat ggacggtggt cagattaaca tcagtggctt ggttagcgat aacacctgtg
      121 aaacaaaagt tgatggcggc aagcaagatg gcctggttct tctgcaaact gcaacggttg
      181 ctgaaattac cgctggtgta tctgaaactg ctgtcggtgc caaagcgaaa ccgtttagca
      241 ttactgtaga ctgcagcaaa gcgactccgg gaggccaacc gactctgaca ttcggttcag
      301 ttttctttgg taacagtcgt ggcactttga ataacgatat gtccatcacc accccgtcat
      361 ctggtgtgaa tatcgccctg cacaacattg acggcaccac tgttaaacaa gtcaaaatta
      421 ataatgcatc tgatgcttac actaaagcat taaccaataa tgttgcagtc tttgacttca
      481 aagcctccta tgttcgtgaa gacgactcaa caccagcaac ggctggttat gtaagaacca
      541 acacagctta caccatcacc tacaattaag ttattcgttg gtgatttaaa ttactacaag
      601 ggagcgacac ctattgtcgc ccccttgtct tgaaaacaat tcatgtcatg gatggtatat
      661 tttgttaaat aaaaagaata ttgtcgcatt atgtgttgcc agtagtatgg catttagcat
      721 gcctgttatg gctgatattg ttatttccgg tacacgcgtc atttataaaa gtgatcaaaa
      781 aagcgttagc gtacgcctgg agaataaagg caatagccac ctgttagtac aaagctggct
      841 ggataccggt gacgataacg ctgagccagg aagcattaat gtaccattta cggcaacacc
      901 tcccgtttca cgtattgatg ctaaacgcgg tcaaaccatc aaattgatgt ataccggcag
      961 taccgtactc cccagcgata aagaaagcgt attctggttt aacgtgctgg aagtgccccc
     1021 aaaaccagat gcttcaaaag tagagaatca gagcctgtta caactggctt tccgtacccg
     1081 tatcaagctg ttctatcgtc cggcaggatt aaacggtaac ccgtctgaag caccattagc
     1141 cctgaaatgg aaatgggctg aagggaagtc tggcctgagt gtatataacc caacgcctta
     1201 ttacgtctct ttcaataacg cagagttaat cgctggcggt aagtcgaacc cgctcaatgt
     1261 aaaaatggtt gcaccgaaaa gcgaagagat tttcaccata agcggttcag gcgggaaagc
     1321 aacgtcagga aaggtacatt tctccgcgat taatgatttt ggcggcgcaa ttgaaggcga
     1381 tgtcaatttg taagaccagg gaccttatgc agcaggaagc caatgtaatg aaaacaacga
     1441 catcgactaa acgccataat cttacgcgta tcgcaatgta ttgctcaatc atgtatagcg
     1501 gcgctaccat cggagccgaa agtgttgagt atgaccctac ctttttaatg ggtggcaacg
     1561 cttccagtat tgatgttagc cgctatagcg atggcaaccc gactttgcct ggtgtttacg
     1621 atgttagcat ctatgttaac gagcaacctg ttgctaacct ggagatcccc tttatcgcga
     1681 tccccgataa aaagaatgct caagcctgta ttaccctgaa aaatctacta caactgcata
     1741 tcaagacccc cccggcagac gaggaaaata ctatccttct tccacgggat gaaacgcttg
     1801 gcaactgtct tgacctgtcg ttagccatac ctaaatcctc agtgaactat gacccctctg
     1861 aacaacgtct ggatatcaat gttccccagg catgggtgat gaagaattat caaaactatg
     1921 tggacccatc actttgggaa aatggcatta acgcggctac gctgtcttat aacatcaacg
     1981 cttatcgcag tgaaaattca aattatacaa atgatagcgt ttatacatcg ttcaatggtg
     2041 gcgtcaattt aggggcctgg cgtctgcgta gcagcggtaa ctatagctgg agaaacgacg
     2101 cgggaagcaa tgttgagttt atgaatcgct acgtccagcg cgacattacc gctattcgct
     2161 cgcagctcat tatgggggaa tcctacacca caggtgaaac gttcgactcc gttagtatcc
     2221 gtggcattcg cttatacagc gacagccgaa tgctgccgcc tgtattggca aacttcgccc
     2281 cgacaattcg tggtgtagca aatactaacg ccaaagtcag catcacccag agtggataca
     2341 aaatttacga aaccactgtt ccaccgggac cttttgtcat tgatgatctc agcccgtcag
     2401 ggtatggcag cgatcttatc gtaaccattg aagaagctga tggcagcaag cgtacatttt
     2461 ctcagccatt ttcttcagta attcaaatgc tgcgcccggg tgtttctcgc tgggatatca
     2521 gcggcgggca aattaataaa gacgacttgc atcatgaacc aaacttacta caagcgacct
     2581 attaccgtgg tttaagtaac cttttcactg gctatactgg ttttcaggtc acagataatc
     2641 attatgctgc aggcttactt ggcattggga tgaatacttc tgtcggcgca atttccttcg
     2701 atgtcaccca ctccagtgtc gatataccgg atgataaaag gtaccagggg caaagctatc
     2761 gcatctcctg gaacaaattt tttgacctga cagatacatc attaaacatc gcggcttacc
     2821 gctactccac gcaggactat ctgggcctga atgatgcatt aacacttatt gatgaagttg
     2881 aacatcccac tcaggatctt gatcccaaaa ctatgcgcaa ctacgggcgc atgaaaaacc
     2941 agttcaccgt aagcattaac caaccgctac gctttgggaa ggatgattat ggctcgttct
     3001 atacatctgg aagctggtct gattactggg ggggcagtaa gagccgtagc aactattcgg
     3061 tcggctacag taacagcgct tcatggggaa gctacagtat cagcgctcaa cgatcctggg
     3121 atgagtacag ccaaacagaa aacagtattt acctcagctt ttcgatacct atcgaaaagc
     3181 tgatgggtac ggagcaccgt gattccggtt tccaaagtat tgatacacaa cttagtacca
     3241 atatggatgg taataatcag ttcaacatga gcagtagcgg ttatagcaac gataaccgcg
     3301 tgagctatag cgtgaacgcg ggttatggga tgaataaaac cggcaaagat ctcagcaaca
     3361 ttgggggtta tgccagctat gaatcgccat ggggaacgct agctgggtca gtctctgcta
     3421 ccagcgataa taaccgccag tactccgtta atacagatgg tggatttgtg ttgcacagcg
     3481 gaggactgac gttcagcaac gacagtttca gtgataatga caccattgct ctggttaaag
     3541 caccgggtgc aaaaggggcg cgtattaact acggcaacaa taccgttgac cgttggggat
     3601 atggcgtaac cagtgctctt tcaccatacc aggaaaacaa aattgctctg gacactgaaa
     3661 acctggaaaa cgacatcgaa atgaagagca ccagtacggt tgccgtacca cgccagggct
     3721 cggttatctt tgctggtttt gaaaccaatc agggacaatc tgcgattatg aatatcaaac
     3781 gcagtgacgg aaaaggtatc cccttcgccg ccgatattca tgatgaaaca aatgcaatca
     3841 ttggcaacgt tgggcagggc gggcaggcat ttgttcgcgg tattcagcag cagggcacgg
     3901 taaaaatcac ctggctggaa gccagcatgc ccaaaacctg tattgcgcag taccagcaac
     3961 ccaatgtgac atcggagaaa atccagcaaa cgattattct caataatatt ctttgccagc
     4021 tccaataata cgaaagagag atgacaatga taaaaaacac acggtataaa atagtactcc
     4081 tggcaggcat ttttttactg cccggcgcac tggcacaaga tattaatgtt gattttgttg
     4141 ccacaatcaa aggcacgaca tgtaatatca ctctggaagg gacacgcgtc accgctgatg
     4201 gcaataatca gtacacgcta cggatattaa atgttgctct ggataaaatc ataaacaaga
     4261 tgccagaagc acaagccgac tttaaactgg tcgctaattg tgatggtaac ataggcaaga
     4321 ttacgacatc gctggcaggg aatccttcag tagagttacc ctatttaatt tctccattaa
     4381 cttcagataa ttcatccacc acagaatata ttgctatggg gattaaactt cgaaatgcct
     4441 ctgacactga atttattccc ccagatggtt ctaaaaagat ttcctgggga gagggattaa
     4501 caaaaccgga acttgagatg acggtagcac ttcgcgaaac ccgaccaggt tcaggaagag
     4561 caggtaattt taaagcactt gcgacattca atttcaccta cgaataaaag gataataata
     4621 atgcagatga aatcattaca ttatgggata tttgcaagca ttactctgag cttattatta
     4681 ttcagcacgt tttctatagc catcacggat aacattggcc taaacgttat gaccactgtt
     4741 gaaaacagaa cttgtcaaag cgaaatcagc aaccaggggt tggttaatct tggcactgtt
     4801 ggagtgggat attttgccag taatgttagt gcggaagata attatccagg cggaaagacc
     4861 tttactgtga cagtaaaaga ttgcccctcc actggcacaa catcaagcca gttgcatata
     4921 gaatttaaac ctgtcagtgg tgtaatggcc actggtagcc aacagatatt cgccaataac
     4981 catgtcggcg gtgcacaaaa tgtaggaatc gtgattttta gccaaaataa tcagtcatcg
     5041 gacacagtga atgtgctgtc accaacaggg cagtttcgca cagtctggcc agttgcctct
     5101 gcaaacatga ataattcatc gtggcaattt tatacacgaa tgcaaaaaat tgatccgtta
     5161 cttcccgttg gggcaggatt tgtcaccagc aatgtattag ttgatattta ttatcaataa
     5221 atgccagaca taacgaatct acaaatatta ttttttcaaa ggagtgataa gatgaagtca
     5281 gcgttccgat actttctttt tttatggatt atcacagcca gcattaataa tgtaaatgct
     5341 ggaaatctta cttttatggt cggtgacgat gctggcgtag gaaaccttaa tggcccatca
     5401 ggcgataaac taatatcatt tgcctatccg gttggtggag taaacaatat tgtattttat
     5461 aatccaacat ccgccacacc gaccaatgca caaataaaat tatattggag tgaactggac
     5521 acatcaagta gccgtgggtt tgtatattgt acttcaaaaa gtggttccag cggtatgcca
     5581 attactgtcc ggcataacat ggtggactca ggaaaaatgg cagatgggca cagattatat
     5641 gaaactaatg taaccggtct atattatacg ttaatgctca atagattctg gtcgtatgaa
     5701 cttcgctcaa gctctccaga tatatacgtt ggagacgatg gctggcacac tgtacaattt
     5761 tctattacgg gtgattatat acgaggtcaa tgtaatactt ccaggtatca agcagtcgga
     5821 ggtcttttgc acgatgccac ggtggaattc tataccgaca gcagttttaa ccctgctcca
     5881 aatactaacg tgcaattaaa acatactggt gattatttgt attcattcgc aaatgaaggg
     5941 ccagggattc caaatgtaag tgcatctaag atgctaaaaa tcaattttaa tttaacagac
     6001 atcaatatcc aactgccaac atgctttacg tctatccttt tgggtgagag cgtaacgcga
     6061 tcaaccgtaa atctcgggga atatactgtt ggacagatca cctcagattc tgcaacacct
     6121 gttaattttc agatctcgct acaaaattgt gttcgtataa gcagtattaa aacaaaacta
     6181 aaatctaatg tagtaggagt gaataatcct ctgttgcttg gtaatacttt gacaggaacc
     6241 gatgatgcaa aaggtgccgg agtacttatt gaagggttac ccaacagcaa aaattcacaa
     6301 aatttcacgc ttaagccaaa tgatgagaca tcaagctata acgatgctga agatgatcct
     6361 ggagaaaaca atggaattta taacccagat tatcctaatg ccaacggtcg tactacaacc
     6421 caggacctga aatttcaggc aacattaaaa cgcgatggta atgcaactgt gaaacccggc
     6481 ggatttaagg ctaccagtac attccaggtc acctacgagt aacaggttta accgcaccgc
     6541 catcatctgg cggtgctgta attgataaat cattgccccc accaatgtga ctgataaaat
     6601 gccagacagc acgacattac ttatcaggat acgttatgaa accaaccact attgcttcat
     6661 tgcaaaaatg caaacaagaa aaaaaacgtt tcgcgaccat caccgcttac gactatagct
     6721 tcgcaaaact gtttgccgat gaagggctta acgtcatgct ggttggcgat tcactgggca
     6781 tgacggttca ggggcatgac tccactctgc cagttaccgt tgccgatatc gcctaccaca
     6841 ctgctgctgt acgtcgcggt gcgccaaact gtctgctgct ggctgatctg ccgtttatgg
     6901 cgtatgccac acaagaacaa gcctttgaaa acgccgcgac tgtcatgcgt gccggtgcca
     6961 acatggtcaa aattgagggc ggtgagtggc tggtagaaac cgtacaaatg ctgaccgaac
     7021 gtgccgttcc ggtttgtggt catttaggcc taaccccaca gtcagtaaat attttcggtg
     7081 gctacaaagt ccaggggcgc ggcgatgaag cggccgatcg actgctcagc gatgcattag
     7141 ctttagaagc tgctggggca caactgctgg tactggagtg cgtgccggtt gaactggcaa
     7201 aacgtattac cgaagcactg gcgatcccgg ttattggcat tggcgcaggc aacgtcactg
     7261 acgggcaaat tctcgtgatg cacgacgcat tcggcattac cggcggtcat attcctaaat
     7321 tcgccaaaaa tttcctcgcc gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg
     7381 ctgaagtgga gtccggcgtt tatccgggcg aagaacacag tttccattaa ggagtgacgt
     7441 tgtgttaatt atcgaaaccc tgccgctgct gcgtcagcaa atccgccgcc tgcgtatgga
     7501 aggtaagcgc gtggcgctgg tgcccaccat gggtaacctg cacgacggcc atatgaagct
     7561 ggtcgacgaa gccaaagccc gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat
     7621 gcagttcgac cgcccggaag atctggcacg ttatccacgg accttgcaag aggactgcga
     7681 aaagctgaac aagcgcaaag tggatttagt tttcgcccct tcggtaaaag agatctaccc
     7741 gaacggtaca gaaacccaca cctatgttga tgttcccggt ctttctacca tgctggaagg
     7801 tgccagccgt ccgggacatt ttcgcggcgt ttcgactatc gtcagcaagc tgttcaatct
     7861 ggtccagccg gacatcgcct gcttcggtga aaaagatttt cagcaactgg cgctgatccg
     7921 caaaatggtt gctgatatgg gctttgatat tgagattgtc ggtgtgccaa ttatgcgcgc
     7981 caaagacggt ctggcgctga gttcccgtaa cggttatctg acggcggaac aacgcaaaat
     8041 tgcgcctggt ctgtacaagg ttttaagttc gattgccgac aaattgcagg ctggcgaacg
     8101 ggatctcgat gaaattatta ctattgccgg gcaagaactg aatgaaaaag gcttccgcgc
     8161 cgatgatatt cagattcgtg atgccgacac cttgctggag gtctctgaaa acagcaaacg
     8221 tgcagtgatt ctggtagccg cctggcttgg cgatgcccgc ctgatcgaca acaaaatagt
     8281 cgagctggtg taatacttaa ctggcgctac ggctgatggc gccagttatt cattgccacg
     8341 ttatctgtaa gcgataattc ataacgcaga caacttagtt agagttaatt agcttatcaa
     8401 cctctgtaag agataaagtt gccatctctg caacgtcttc ccgagacatt cctttactca
     8461 gaagacgctg tgccagcctt accttccgga gtccctcttt atatcccttc tgaaatccct
     8521 tctgaaatcc cttctcttca aaccactgcg ccaacgtctg cctggtttct ctccgtgtct
     8581 ttatttccat aggttccctt aattatctgg tcagttaaca ataacaatta tctggctata
     8641 actgatgatg cagacaactt agttaatcag cttatcaatc tcggcaaggg gtaaatttgc
     8701 catctctgca acgtcttccc gagacattcc tttactcaga agacgctggg cgaattcctg
     8761 acttacttcc tgtgttactt cctgtattac ctcttgtctt acctcttgtc ttacctcttg
     8821 tcttacctcc tgtcttcctt cctgtcttcc cctctcttca aaccactgcg ccagcgtcat
     8881 catagactcc cctcccgttt ccctgtctct cagcacacca taaaacaaat ccgcttgttc
     8941 agtatgaccg cgttgcagca tatagttttg catggcaact aactgacttc cgctagtgta
     9001 cccttcgtca atcagcgtga ccagttgctc cagcaataac attaagtcgc gctggcgaat
     9061 atgtttttgc agtagttcga gaatcgcaat ccgccgatgc tgcatgattt cgtcatccgg
     9121 tgtgatggtg atatccacca gcgggaaagg gttgttgtaa acgcgtcgcg ccagctccgg
     9181 cgagtaaaac atatcaaacc agcacattga gagcggataa ggtgtggcct cgccctgata
     9241 aaacagaatc ggcaccacca gcggcagctt atcgtgatcc gcctccagat gccggtgcat
     9301 ggcagcaata gaataacgca tcatgcgaaa ggccattttc ttatccggct tgctttgatg
     9361 ttcaatcacc acatgcaaat aaccgggatt accctgcatt tgcacggaat agagcacgtc
     9421 agtgctgtgc cctttcaggc tttcttcaat aaaactcccc gactcaagat gcagcgtgtc
     9481 gaggtcacac agttcgcgta gttccgctgg caaatggata tccagaaagt cacgagccgt
     9541 ttccgcatgc attaaaaatt gtttaaatac cgcgtcatgc ggtgtggtac tcggtgcatc
     9601 catcttccct gccttccgtg ttgtcttgcg tgaaggcagt atccagtcaa ggcgcgccgt
     9661 cgtcagccgc ctctttattg tttaccgagc agcgttcaga gaaatttcaa ttggttacag
     9721 gcgttacatg gcacgcttcg gcacggtacg caaactgctg atgcaaatgc cgtcagatga
     9781 gtagacacta aacaaaaatc gggcaatact gcgtgagaat ttcctcaaag caggcatcgc
     9841 ctgcttcgtt aacgacaggg tagaaaggta gaagttatga ttcgcacgat gctgcagggc
     9901 aaactccacc gcgtgaaagt gactcatgcg gacctgcact atgaaggttc ttgcgccatt
     9961 gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg aagccattga tatctggaat
    10021 gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt
    10081 atttccgtta acggtgcggc ggcccactgc gccagcgtcg gcgatattgt catcatcgcc
    10141 agcttcgtta ccatgccaga tgaagaagct cgcacctggc ggcccaacgt cgcctatttt
    10201 gaaggcgaca acgaaatgaa acgtaccgcg aaagccattc cggtacaggt tgcctgataa
    10261 tcacccctgc ggctggttac tcaccagccg cgacattgtc tccagcgaat ccgttcttaa
    10321 gatataaagt cgttttagta acaacggatt atcccccggt tttactttgc ctttcatggt
    10381 tgtcaccgcc aggtgaaatc cggcttcttt tgctgccttc acggctttgt cattaaatcc
    10441 gccaaacgga tacgaaagat accagacatg cggattaaat tgcgccagag cgcggcgtga
    10501 acgtgcaaaa tcaaacagga tattgtgctc actacggctc agtaatatgg gtcggcgata
    10561 accatccacc cgatgcaaaa aatgggtatg tgactggaaa tcaaatacat cgcgaatttc
    10621 gttaagttca gaaacgctca taaattgcag cgattttggg tcccacttct gcgggtgacg
    10681 tttgatgcgc gaggtaacaa taaacgccgt cgccttcatg ccatattgtt tcaacacagg
    10741 atacgcatag cggctcaccg actttaggcc atcatcaaag gtaatcacca ccgctcgcgc
    10801 agggagattg atcttattct tcacgtaacc ttccagttgc gccatgctca gtgtcacgta
    10861 tcccctgtcg cgcagccagg ccatctggtt attgaaagcg cgtaccgagg tggtcgtcga
    10921 agtatggcga aaacgggtat tttcttcgtc gcgcagaata tggtgatagg ttagcaccgg
    10981 caggccatta tcgggttggg catccagcgc gctgatatag gccagtcgat cgccaatacg
    11041 gatctgatac caggtttgat ttaacctgtc tttcagttta tgcaaaattg ggtagcgcaa
    11101 attgtccgcc agtaccccaa acggcgcact gcccgcactc ggcgcgttga agaccggcgt
    11161 atctttccag gttattaagt tctgattact cagcggcttg ttgaggtcac ccaaaccatc
    11221 ttcaactttt tgtcgcccct gaaccggctc aagatgacct ttatcgataa accctttacc
    11281 aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa tgatttgccc
    11341 ggcccgaata ttccccacgg tcaccatctt gtcgccaatt tgcgcccaga ccgcagcatt
    11401 ttcgatggtt tgcatataac gggcaggtaa tgcggcactg acgcttgcgg taaacagcat
    11461 cagcaggaga ataacagctt gtttgtacat gaaaaaataa ccgagggtga aacaacgctt
    11521 ttattgtagc aaaagtgcca tcagaatcgc gggttaatac tcatacaaat tatgtttgag
    11581 gacgaaaggc ggattttttt gttgctggtg ccaaagacta ctcacctccg gcgcacccag
    11641 ttcgacaata cgctcgcgaa actctgcgct ggataacgtt tcacggtaga ccagcatctg
    11701 ctcaattaac ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt tgtggcttaa
    11761 taatgaagcc acgcggtacg gtggcgctcc ggctatatcg gttaagaaga tgacaccctc
    11821 accagagtcc gcttcatgca gagcatcgca catcatgcgg ctgagcatat tagaacttaa
    11881 tccgcaccag aaattcacgg cccggcactg aagaagtgcc ccatgttttt tctccagcgc
    11941 atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta acatcgcctt
    12001 cctccttagc cgaaaatcag tctaccctga ccattgttga taaagggtga tagccgtcaa
    12061 aactcctctc ccccaaatcc agaggagagg aaatagcctt agctacgcaa accacgtcca
    12121 cgttggatca gcgaccaaca gatcaaataa aacgccacaa taaagaccac cagtacaccg
    12181 aaggtagtga ccagcggaac atcattgata ccgaggaagc cgtagcggaa accactgatc
    12241 atataaacga ttgggttcag gtgcgacagc ccttgccaga acggcggcaa caaagtcagt
    12301 gagtaaaaga ccccgcccaa atacgtaagt ggcgttaaca caaaggttgg caccaggctg
    12361 atgtcatcga acgttttggc aaacacgccg ttcagcaaac ccgcaaggga gaacaacacc
    12421 gccgtgagca ccagcgttaa agcgacaaat acccacgaat gcacctgaaa cggcacaaaa
    12481 aacagtgaaa ttgccgtcac cagaatgcca acaaacagac cacgcgccac gccaccgccg
    12541 acatatccgg caataatgac gtgagtcgga accggcgcta ccagcagctc ttcaatatta
    12601 cgctggaact tggcaccaaa aaatgatgac gcgacgttag cgtaggcgtt ggtgatcacc
    12661 gacatcatga tcagccccgg tacaatgaac tgcatgtagc tgaagccatg catatcgcca
    12721 atacgcgaac caatcaggtt accgaagata ataaagtaaa gggtcatggt gatgaccggt
    12781 ggcaccagcg tctgcaccca gatacgcata aagcgatgga tctctttcgc ccagatgctt
    12841 tttagtgcca cccagtaaag atgcatcatg cgcgatctcc ttgtttttca ttaaccagtg
    12901 aaacaaacag ctcttccaga cggttagctt tgttacgcat acttaatacc tgaatgccct
    12961 gctcacttaa ctgcgtaaat acgctgttga tcccctgctc acgcagcact tcaacttcca
    13021 gcgtcgcggt atcgaccagt cgatactgat agccatcgag cttcggtaac gggcttttcg
    13081 gtgcgagatc gagaataaag gtttccgatt tcagctttgc cagcagcgcc ttcatcgagg
    13141 tattttccac cagctcaccg tgttgaataa tgccgatatt gcggcacagc atttctgctt
    13201 cttccaggta gtgtgtggtg agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa
    13261 agccccacat tgagcggcga agttcaatat ccacgcctgc ggtaggctcg tcgagaatca
    13321 gtagtttagg ttcatgcatt aacgcacggg caatcattaa acggcgcttc atcccgccag
    13381 ataacatacg cgcacgttcg ttgcgttttc cccatagatc gagctgttta agatactttt
    13441 cgctgcggat gtacgcttct ttgcgctcca caccgtagta ccctgcctga ttcaccacaa
    13501 tttgctgcac ggtttcaaac gggttgaagt taaattcctg cggcaccagt cccaactgac
    13561 gtttagcgtt cacgacatcc ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg
    13621 ttttatttac tagagagctg ataataccga tagtggtcga tttcccggcc ccgttcggcc
    13681 cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc tattccacga agcgcctgaa
    13741 cgccgcctgg ataggttttt ttaagctgtt gaagttccag tgcaatggtc ataaattttt
    13801 acttacctta cgttcttaca ctttatctgt ggtttaaatc gtcccggagt tgccctatat
    13861 tagccaaacg taattatttg gttacaggtc gttaacctcc atgaaagaca tagatacact
    13921 catcagcaac aatgcactat ggtcaaaaat gctggtggaa gaggatcccg ggttttttga
    13981 gaaactggca caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt
    14041 tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc
    14101 taacctggtc attcacactg acctgaactg cctttccgtg gttcagtatg cagtggatgt
    14161 actcgaagtt gaacacatta ttatctgtgg ccactacggt tgtggcggcg tacaagccgc
    14221 agttgaaaac ccggaactgg ggcttatcaa caactggctg ctgcatatcc gtgatatctg
    14281 gttcaaacat agctcattgc tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg
    14341 tgaactgaac gtcatggaac aggtgtataa cctgggccac tccaccatta tgcaatcagc
    14401 gtggaaacgc gggcagaaag tcaccattca cggctgggcc tacggcattc acgacggctt
    14461 gctgcgtgat ctggatgtca cagcgaccaa ccgcgaaact cttgagcaac gttaccgtca
    14521 cgggatttcc aatctcaagc tgaaacacgc caaccacaaa taaaaatgcc atgccggatg
    14581 caacacatcc ggcaacttca cacttactcg tccagcagaa tcactttgcc gatatacggc
    14641 agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc atccgggatc
    14701 gagaaaccga tgaattctac cgggacgttc acttcacgac gggacggttt atccagcagc
    14761 gtacaaatcg ccaacgactt cggttcgcgc aggcttaaga tctcacgcac tttcgacagt
    14821 gtattacccg agtcgatgat atcttcaaca atcagcacgt ccttgccacg gatatcttca
    14881 tccagatctt tgaggatttt cacatcacgg gtggtggaca tgccgctacc gtagctggag
    14941 gcggtcataa aatcgacttc atgagatacc tgaacttcac gacacaggtc cgccataaac
    15001 ataaatgagc cacgcagcag acccaccagc accatatcgc tgccgctgtc tttgtaacgc
    15061 tcagtaatct gacgacccag ttcggcgata cgcgctttaa tctccgcttc ggggatcatt
    15121 acttctacag tatgtttcat atctctaacc atatgattta aaaacaaatc acttcgcacg
    15181 ggcaggtttt ggcaccgcaa caaagcgcaa gacacgcagt ataccagtaa aactattcat
    15241 tcgcggaatc tgttaataaa gctcgaattg tgatgacgat cacacatgtt aaaacctata
    15301 attaattgct actaaaatat taatgaattg aaatggtgtc tctttatggc aattaacaat
    15361 acaggctcgc gacgattact cgtcacgcta acagcccttt ttgcagcgct ttgcgggctg
    15421 tatctactca ttggcggagg ctggctggtc gcgattggcg gctcctggta ctaccctatc
    15481 gctggccttg tgatgctcgg cgtcgcctgg atgctgtggc gcagtaaacg cgccgcgctt
    15541 tggctatacg ccgccctgct gctcggcacc atgatttggg gcgtctggga agttggtttc
    15601 gacttctggg cgctgactcc gcgcagcgac attctggtct tcttcggcat ctggctgatc
    15661 ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc cgctctggtg
    15721 gtggcgttgc tgattagtgg cggaatcctc acctgggcgg gatttaacga tccgcaggaa
    15781 atcaacggca ccttaagcgc cgatgccaca cctgccgaag ccatctcacc ggttgccgat
    15841 caggactggc ctgcgtatgg tcgcaatcag gaaggtcaac gcttttcgcc gctgaaacag
    15901 attaacgccg ataacgtcca taacctgaaa gaagcctggg tgttccgtac tggagatgtg
    15961 aagcagccga acgatccggg cgaaatcacc aatgaagtga cgccgattaa agtgggcgac
    16021 accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc tcgatgccgc cagcggcaaa
    16081 gagaaatggc attacgatcc tgaactgaaa accaacgagt ctttccagca cgtaacctgc
    16141 cgtggcgtct cttatcatga agccaaagca gaaacggcct cgccagaagt gatggcggat
    16201 tgcccgcgtc gtatcattct tccggtcaat gatggtcgcc tgattgcgat taacgctgaa
    16261 aacggcaagc tgtgcgaaac cttcgctaat aaaggcgtgc tcaatctgca aagcaatatg
    16321 ccagacacca aaccgggtct gtatgagccg acttcgccgc cgattatcac cgataaaacc
    16381 atcgtgatgg ccggttcagt caccgataac ttctcaaccc gcgaaacgtc tggcgtgatc
    16441 cgtggttttg atgtcaacac cggggagctg ctgtgggctt ttgatccggg cgcgaaagat
    16501 ccgaacgcaa tcccgtctga cgaacacacc tttaccttta actcaccaaa ctcgtgggca
    16561 ccagcggcct atgacgcgaa gctggatctg gtctatctgc cgatgggcgt gaccacgccg
    16621 gatatctggg gcggtaaccg cacaccggaa caggaacgtt atgccagctc gattctggcg
    16681 ctgaatgcca caaccgggaa actggcgtgg agctaccaga ccgttcacca cgacctgtgg
    16741 gatatggatc ttccggcaca gccgacgctg gcggacatca ccgttaacgg tcaaaaggtt
    16801 ccggtcattt atgctccagc gaaaaccggc aacatttttg tgctcgatcg tcgtaacggc
    16861 gaactggtgg ttccggcccc ggaaaaaccg gttcctcaag gtgcagcgaa aggcgattac
    16921 gtcaccccaa ctcaaccctt ctcggaactg agcttccgtc cgacgaaaga tttgagcggt
    16981 gcggatatgt ggggagccac catgtttgac cagctggtgt gccgcgtgat gttccaccag
    17041 atgcgctatg aaggcatttt caccccacca tctgaacagg gtactctggt cttccctggt
    17101 aacctgggga tgttcgaatg gggcgggatt tccgttgatc cgaatcgtga agtggcaatt
    17161 gccaacccaa tggcactgcc gtttgtttcg aaactgatcc cgcgcggtcc gggtaatccg
    17221 atggaacagc cgaaagatgc caaaggcacg ggtacggaat ccggcattca gccgcagtac
    17281 ggtgtaccgt atggcgtcac gctcaatccg ttcctctcac cgtttggtct gccatgtaaa
    17341 cagccagcat ggggctatat ctcggcgctg gatctgaaaa ccaatgaagt ggtgtggaag
    17401 aaacgtattg gtacgccgca ggacagtatg ccgttcccga tgcctgtacc ggtgccgttc
    17461 aatatgggta tgccgatgct gggcgggcca atctccacag cgggtaacgt actgtttatc
    17521 gccgctacgg cagataacta cctgcgcgct tacaacatga gcaacggtga aaaactgtgg
    17581 cagggccgtt taccggcggg tggtcaggct acgccaatga cctatgaagt gaatggtaag
    17641 cagtatgtgg tgatctccgc aggcggtcac ggttcatttg gtacgaagat gggcgactat
    17701 attgtggctt atgcgctgcc ggatgatgtg aagtaaccat aatgcccgga gagatccggg
    17761 catatttccg aatacggtct ttttataccg taaaccctaa catcatcccc gtatcttcat
    17821 gctccagcaa atggcagtgc gccatataag catgttcttt cggtgcatcg tgattaaact
    17881 tcaccagcac ttcgctgaca ttaccttcta ccttaacggt atctttccag cctgcgcgat
    17941 gagtcgctgg cggtttgcca ttttctgaca agatacggaa ctgcgtgccg tgaatatgga
    18001 acggatgcag catcatgtcg cccacgccag agataaccca acgttcgtac tgccctttcg
    18061 ccgccgcaaa catcggctta ttcatatcaa acgcctgacc gttgatttta ttggcatggt
    18121 ggaaatcgaa cttcccgccg tggttcatat gattcatatt gccgtgcccc atatggccca
    18181 tcatctggct gtgatccatc ccggccatcg cctgatcgcc atatttctcc atcagcatct
    18241 gcatccccat catatcgagc atcgggtcca tagagagttg cagcttacgt accgtcagcc
    18301 cttccagcga aggtaatgct ggcaggctac ttaatgtgtc tggcaaagca ccagaggcac
    18361 taatggcaat cggctgaatc cgcattaccg gatgcggctt atcaaaaggc gcaatcgcca
    18421 ttcccatctg gctgaccggc agcgtcacca ggtcaaaggg gttgttgtcg ttaacctcca
    18481 ccagcacttc aaaacgctcg cccatcagca ccggcagctc gctcaccttc accggttcag
    18541 gtagtagacc accgtcgctg gcaatcacat acagcgggcg attgtcgctg gtggcgaaat
    18601 tgagcgagcg ggcgttacag ccattgagca aacgcaggcg cagccaacca cgcggggcag
    18661 cgtgttgtgg gtagattgca ccgttggtca gcaacgtatc gccaaaccag cccacggcgg
    18721 cggtcatcac atccagttga taatcaatct gcccgtcagc gctaaatttc ttatcctgaa
    18781 cgatcaccgg aacatcatcg ataccccact gttttggcag cattaatttc aggatctcgt
    18841 catcttcaat caccaccaga ccagccagcc ccatcgccac ctgtcgcccg gtcttgccat
    18901 gttgatgtgg atggaaccag caggtagcgg caggttgatc aacgttcaac gtcaccgagc
    18961 gcttgccacc tggcggaata attccctgcg ggccgccgtc gacttcaccc ggtacttcca
    19021 gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt atagatatca accgttaccg
    19081 ctttgccgcg ctgtaatttc accgccggcc ccagcagatt gccgttatag ccccaggtag
    19141 ttgcggtttt cccgccaaag gtggactggc ctgcgccaat agttaactga atgcgattac
    19201 gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg gcgttccgcc gcaaatactg
    19261 cgcggctcca cagcggtaaa gccgaagcca cgcccagcgc gacggaatat tttaagaaat
    19321 cacgacgttg catagttatt tccttattct taagcaggcg aaacaaaatc aaacgttgag
    19381 catagtcctt ccccttacgg gaaggtcaag ccgcagacac aatttaatcg acaataatgg
    19441 tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg tagaagcaat
    19501 gaagacgttt ttcagaacag tgttattcgg cagcctgatg gccgtctgcg caaacagtta
    19561 cgcgctcagc gagtctgaag ccgaagatat ggccgattta acggcagttt ttgtctttct
    19621 gaagaacgat tgtggttacc agaacttacc taacgggcaa attcgtcgcg cactggtctt
    19681 tttcgcccag caaaaccagt gggatctcag taattacgac accttcgaca tgaaaaccct
    19741 cggtgaagac agctaccgcg atctcagcgg cattggcatt cccgtcgcta aaaagtgcaa
    19801 agccctggct cgcgattcct taagcctgct tgcctacgtc aaataatccc tgataccttt
    19861 ttgtagaaat attgaccgtg catccgcggt caatgttagc tattatgttg cgcccttttt
    19921 ttacgggtgt taacaaagga ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac
    19981 gctacacgac cagtttgggc agtactttgc ggtagataac gttctgtatc atgaaaagac
    20041 cgatcaccag gatctgatca tttttgagaa cgctgcattt ggccgcgtaa tggcgctgga
    20101 tggcgtagta caaaccaccg agcgcgacga gtttatctat catgagatga tgacccatgt
    20161 tccgctactg gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc
    20221 catgctgcgt gaagtgaccc gacataaaaa cgttgagtca atcacgatgg tggaaatcga
    20281 tgcgagtgtc gtgtcgttct gccgtcagta tctacccaac cataacgctg gtagctacga
    20341 cgatccgcgc tttaaactgg tgatcgacga tggcgtcaat ttcgttaatc aaaccaacca
    20401 gacttttgat gtcattatct ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt
    20461 cacctcagca ttttatgaag gttgtaaacg ctgcctgaat cctggcggta tcttcgtcgc
    20521 acaaaacggc gtctgctttt tacagcagga agaggctatc gacagtcatc gcaaactcag
    20581 ccattacttc agcgacgttg gcttttatca ggcggcgatc ccgacctatt acggcggcat
    20641 catgaccttt gcatgggcga cagataacga tgccttacgc catctctcaa ccgaaattat
    20701 tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat tacaatccgg caatccatac
    20761 ggcagctttt gccttacctc agtatctgca agacgcactg gcttcacagc cgtcctaagg
    20821 agaagataag aaattgaaaa aactgaaact gcatggcttt aataatctga ccaaaagtct
    20881 gagtttttgt atttacgata tctgctacgc caaaactgcc gaagaacgcg acggttatat
    20941 tgcttatatc gatgaactct ataatgccaa ccgtctgacc gaaatcttgt cagaaacctg
    21001 ttccattatc ggggccaata ttcttaacat cgcccgccag gattacgaac cacagggtgc
    21061 cagcgtcact attctggtga gtgaagaacc ggttgacccg aaactcatcg acaaaacaga
    21121 acaccccggc ccactgccag aaacggtcgt tgcccatctc gataaaagtc atatttgcgt
    21181 acatacctac ccggaaagtc atcctgaagg cggtttatgt accttccgcg ccgatattga
    21241 agtctctacc tgcggcgtga tttctccgct gaaggcgctg aattacctga tccaccagct
    21301 tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt tttactcgcg acattaacgg
    21361 tatgaagcac tttatcgacc atgagattaa ttcgattcag aactttatgt ctgacgatat
    21421 gaaggcgctg tatgacatgg tggatgtaaa cgtctatcag gaaaatatct tccataccaa
    21481 gatgttgctt aaagagttcg accttaagca ctatatgttc cacaccaaac cggaagattt
    21541 aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta
    21601 ttacgggcgc aatatgcccg ctgtttaacg gctctggcgg agctcgcggg ctccgccaga
    21661 tttatttact tttgctgcac gaaattacga taagcagcca ccacctgcag aaaatcctca
    21721 acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc catttcatcg
    21781 ccagcgaatt ccagttgatt ggcgcgaacc atcacttctt cgccgtccat ccatagcgtg
    21841 tactcatgcc ctgcccgctg ccaggaacgt tcgctacctt tgagcgtgcg cgcggcgtct
    21901 tctacttcat caagcaaggc caggttttct ttcacctctt cgttaaacca gtgcccgacc
    21961 acttcatgcc ccatggacat acgcaccttt accactccgg taatatcacg cagaaattcg
    22021 taatccatag tgtgttcctc tgctcccggc aatgcgccat tactgcattg caccttaggt
    22081 ttaattatcg cagcactatg caagattaaa aggtggaaaa gaataaggaa taaaaataaa
    22141 ccccctttcc ttgagcagaa agggggttta aagaagtgtt acttgtcagg tattcaggag
    22201 aacacctgag ctattttcag gctgttacac cgcagtctgg aaaatcaccc cgtccgcttt
    22261 ttcggtgtac tgagaaagct ggttgaagtt caggtaacgg taagtatcaa cggcagtttt
    22321 atccacctgt gccacgtagg tctggtactc ttccggcgtc ggcagtttgc caatcagcgc
    22381 cgcaacagca gccagttccg cagaagccag gaagacattc gcgccagtac ccaaacggtt
    22441 cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg tctgctacac gcgcctggtt
    22501 acccatacac agggaacagc cagggatctc gatacgcgca ccactcttac cgaagacgct
    22561 gtagtagcct tcttccgtca gttgcgcggc gtccatacgg gttggcggtg ccacccacag
    22621 gcgggtcggc aactggcctt tatgcgcatc cagcagttta cccgcagcac ggaagtgacc
    22681 gatgttggtc atgcaggaac cgataaacac ttcgtcgatc ttctcaccct gtaccgcaga
    22741 cagcggacgt gcgtcatccg gatcgttcgg agcacacagg attggctctt taatatccgc
    22801 cagatcgatg tcgatcactg ccgcgtattc cgcatctgca tcggcttcca gcagctcagg
    22861 attcgccagc catttttcca tgccctgaat acggcgttcc agggtacgac gatcgccgta
    22921 accttcggca atcatccact tcagcaggac gatgttagag ttcaggtatt caatgatcgg
    22981 ttctttgttc agcttaatgg tacaaccggc agcagaacgc tcggcggacg catcggtcag
    23041 ctcaaaggcc tgctcaactt tcagatccgg cagaccttca atttccagaa tgcgacctga
    23101 gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga tcgcatacag
    23161 cggaatagcg tgtaccagat cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg
    23221 caccagaacg gattccggca tatccagcgg catgacgcct gttgcggctg caaatgccac
    23281 cagaccagaa cccgccggga aagagatacc gatcgggaaa cgggtatggg agtcaccacc
    23341 ggtaccgacg gtatccggca gcagcatacg gttcagccag gagtggatta cgccgtcacc
    23401 cggacgcagc gacacaccac cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt
    23461 cacgtcaact ggcttcggat acgccgcggt gtggcagaaa gactgcatca ccaggtcagc
    23521 cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc cggtggtgtc
    23581 ctgtgaacct acagaagtca ttttcggttc gcagtacgcg cccggacgaa tgcctttcac
    23641 gccgcaggca cgacctacca ttttctgcgc cagcgagaag ccgcggtcgc tctcagcgac
    23701 atctttcgcc tgacggaaca catcgctgtg cggcagacca agtgcttcac gcgctttggt
    23761 ggtcaggcca cgcccgataa tcagcggaat acgaccaccg gcacgcactt catcaatcag
    23821 cacgtcggtt ttcagctcga aggtcgccag cagttcgccg gtttcgtggt tacgcacttc
    23881 acctttgtac gggtaaacgt caattacgtc acccatgttc aggttagaga cgtccacttc
    23941 aattggcagt gcacccgcat cttccatcgt gttaaagaag atgggtgcaa ttttaccgcc
    24001 gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc ccataaacca
    24061 cagaacggag ttcgtggcgg atttacgcga tgaacccgta cccacaacgt caccgacgta
    24121 cgccagcgga aaacctttct gttgcagagc ttcaatctgt ttgatcggac caacaacacc
    24181 tggctggtct ggctcaatac cttcacgggc gtttttcagc atcgccagcg cgtgcagtgg
    24241 gatatccggg cgtgaccacg catccggtgc cggagagagg tcatcggtgt tggtttcgcc
    24301 agtgacttta aagacggtaa cggtcagttt ttcagccagc gccgggcgat tcaggaacca
    24361 ttcggcatcc gcccaggact gcattacctg cttagcatat tcattgcctg ctttggcttt
    24421 ctcttctacg tcatagaagt tatcgaacat cagcagtgtg tgagaaagtg ctttggcagc
    24481 aatcggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt tgtaaccacc
    24541 ctgcatggtg cccagcagtt cgatagcttt ttccggagtc agcagagggg atttggcttc
    24601 gcctttagca atagcagcca ggaagcctgc ttttacatag gcggcttcat cgacgcctgg
    24661 gggaacacgg ttggttaaca gatctaacag gaattcttct tcgcccgcgg gcgggttttt
    24721 cagcagctct acaagcgcgg ccatttggtt tgcatccagg ggtttgggcg caatcccctc
    24781 agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc tcctcgctct
    24841 cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca gtttgtaggg
    24901 taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt taatccgttt
    24961 acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag attgtttccc
    25021 tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct ttctcaacga atcccaagat
    25081 tagtcaaaat ttaaactacc gcctctttat actcggattc tcagcacctg cgggtggcag
    25141 ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt gccgtttaaa
    25201 ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg ccgcctctgc cgggctatat
    25261 ataaaaagcc gcacagtgga agcgcctgtg gaagcgcaat cgacacaact gactgtgtct
    25321 gacgctgccg cggttacgct tcctgcaacg gtttccgcgc ctcccgtaac acccgccgtc
    25381 gtcaaatccg cattcaacac tgcacaaata gatcaatggg ttgcgcctgt cgcgctgtac
    25441 cccgactctc tgctttcaca agtgttaatg gcatcaacct atccgacaaa cgttgcgcaa
    25501 gcagtgcaat ggtcgcacga taatccactt aaacaaggcg atgctgctat tcaggcggta
    25561 tctgaccagc cgtgggatgc aagcgtcaaa tcactggtag cctttccaca gttgatggcg
    25621 ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct ggcgcagccg
    25681 caggacgtga tggactcggt acagcgattg cggcaactgg cgcaacaaac cggctcgctg
    25741 aagtcatcaa ccgaacagaa agttattacc acaacgaaga aaactgtacc ggtaacacag
    25801 acagtcacgg ctcccgtcat accatccaat accgttttaa ctgccaacca cgtcattaca
    25861 gagccggcaa caaccgtcat ttctattgag cccgccaatc ctgatgtggt ctatattccc
    25921 aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc gccggtttat
    25981 ctgccaccac cagccggaga accgtttgtt gacagctttg tgcgcggatt cggctatagc
    26041 atgggcgttg ctaccacgta cgcgctattc agcagcatcg actgggatga cgacgatcat
    26101 gaccatcatc atcatgacga tgatgattat catcaccacg atggcggtca tcgtgacggt
    26161 aatgactggc aacacaacgg cgacaacatc aatatcgacg tcaataattt caaccgtatc
    26221 accggtgagc atcttactga taagaatatg gcatggcggc acaatccaaa ctaccgtgat
    26281 ggtgtgccct atcatgatca ggatatggca aagcggtttc atcaaaccga tgtcaacggc
    26341 ggcatgagcg ccacgcagtt acctgctcca acacgcgaca gccagcgtca ggcggcagca
    26401 agtcagtttc agcaacgaac acacgccgcc cccgtcatta cgcgagatac ccaacgtcag
    26461 gcagcggcac agcggtttaa tgaagctgaa cactatggga gctatgacga cttccgcgac
    26521 ttcagccgtc gccaaccact gacccagcaa caaaaggacg ccgctcgtca gcgttatcag
    26581 tcggcctctc ctgagcagcg ccaggcagtc cgcgagaaaa tgcagactaa cccgcagatc
    26641 cagcagcgaa gagaggcagc gcgtgagcgt attcagtccg cctctcctga gcagcgccag
    26701 gcagtccgcg agaaaatgca gactaacccg cagaaccagc agcgaagaga ggcagcgcgt
    26761 gagcgtattc agtctgcaac tcccgaacag cgccaggtgt ttaaggaaaa agtacagcag
    26821 cgcccactga accaacagca acgtgataac gcccgccagc gtgttcaatc agcatcacct
    26881 gagcaacgtc aggtttttcg ggagaaagtt caggagagcc gcccacaacg tctaaacgac
    26941 agtgaccata ctgtcaggct gaataacgag caacggtcag cagtacgcga acgtctctct
    27001 gagcgcggag caaggcgact ggaaaggtaa attacagacg taaaaaaagc ggcgtggtta
    27061 gccgcttttt taattgccat atgtcccggc aaacgaagaa ttacttcttc ttcgctttcg
    27121 ggttcggcag gtcggtaatg ctaccttcga acacttctgc cgccaggccc acagactcgt
    27181 gcagagtcgg gtgcgcatgg atggtcagtg cgatgtcttc agcatcacaa cccatttcga
    27241 ttgccaggcc gatttcaccc agcagctcgc cgccgttagt accgacaatc gcaccaccga
    27301 tcacacggtg agattctttg tcgaaaatca gcttggtcat accgtctgcg cagtcggaag
    27361 cgatcgcacg accagaagca gcccacggga aggtggcagt ttcatagctg atgcctttct
    27421 ctttcgcttc tttctcggtc agacccaccc atgcaacttc tggttcggta taggcgatgg
    27481 acgggataac tttcggatcg aagtagtgtt tcttaccggc gataacttca gcggcgacgt
    27541 gaccttcgtg aacacctttg tgtgccagca tcggctgacc gacgatatcg ccgatagcaa
    27601 agatgtgcgg tacgttggta cgcagctgtt tgtcaacgcg gatgaaacca cggtcgtcca
    27661 cttccacgcc agctttgcct gcgtcgaggt ttttaccgtt cggcacacga ccaatcgcta
    27721 ccagcacggc gtcgtaacgc tgcggttcag cgggtgcttt tttgccttcc atcgtcacat
    27781 aaatgccgtc ttctttcgct tcaacggcgg taactttggt ttccagcatc aggttgaatt
    27841 tcttgctgat acgcttggtg aagactttaa cgatgtcttt gtcagctgcc gggataacct
    27901 ggtcgaacat ttcaaccaca tcaatctgtg aacccagcgc gtggtatacg gtccccattt
    27961 ccagaccgat gataccgcca cccattacca gcaggcgttc tggtacttct ttcagttcca
    28021 gcgcgtcagt ggagtcccag atacgcggat cttcatgcgg aataaacggc agttggatcg
    28081 ggcgagaacc cgctgcaatg atcgcgttgt cgaagttgat cacggttttg ccgttctcac
    28141 cttcaacttc cagggtgtta gccccggtga atttacccag accgttgacc actttgactt
    28201 tgcggccttt tgccatacca gccagaccac cggtcagctg attgatcact ttctctttcc
    28261 aggtacgaat cttgtcgata tcggttttcg gttcgccgaa gacgataccg tgttcagcca
    28321 gcgctttggc ttcttcgatg acttttgcta cgtgcagcag tgctttagaa gggatacagc
    28381 cgacgttcag gcaaacaccg ccaagggtgt tgtaacgttc tacgattacg gtttccagac
    28441 ctaaatcagc gcaacggaag gcagcggagt aacctgcggg gcctgcccca agtaccacga
    28501 cctgagtttt gatttcagta ctcatcatga cctctatata tttatctccg gcggtcatac
    28561 ccgtcgtctt tcaggccgca gcggcgttag cttcacgccc tcaccccagt cacttactta
    28621 tgtaagctcc tggggattct ggcgcttgct accttgctgc acccagaaat ccatagggta
    28681 tgtgaagtaa taccctaacc accaccgggt cgttctatcc gtcggtattt tacaaaattg
    28741 ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata acctcaatac
    28801 attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact tacatcacca
    28861 gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca ccatcagcac
    28921 cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc ggcacgaact
    28981 ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata gccacttccg
    29041 gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg gagatggtga
    29101 agcaaccgcc ctgcatttcg cccgcagtca gcttaccgtc acgcgctttc ttagaaatag
    29161 tcatcagctc gcgagacagc tcgatgatgc ctttcttgtt gacgtctttg aataccggaa
    29221 caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc ttcagggtca
    29281 gacgctgacc gtcttccgac agcgaactgt tgaagcgagg catctgctca agagctgcag
    29341 caacggcttt catgatgaag acaactgggg ttatcttcac atccagctta cgtttcgccg
    29401 cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt ttgtcgaagt
    29461 gggtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa attttctgga
    29521 tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccact ttcggccacg
    29581 gcagcatgcc tgggataccg ccgccagtca ccgccggagc cgcttctgca cgtttgatag
    29641 cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg ccagtgccct
    29701 tcactttcgc caggttaacg ccaaactcgc gtgccagacg gcggatcagc ggagtcgcgt
    29761 gaacgtaagc gtcgttttca gcaaattcag atttgccttc cgctttcaca gccggtgctg
    29821 ctgccggggc ttcagctttt gctgccggag ccggcgctgc cgcttcctgt ttcgcaggag
    29881 ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca gttttcactt
    29941 tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc gggacttcca
    30001 tagaagcttt atcgccttct acggtgatca gtgactgttc agcggcaact ttgtcgccca
    30061 ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga acgttaactt
    30121 ctttcacgcc agctgctgat gcaggggccg ctgccggagc tgcttcctgt ttagccgccg
    30181 gacctgccgc gcctgcttca cccgcgactt cgaagaccat aatcagcgag ccggtagaca
    30241 ctttgtcacc cacgttcact ttgatctctt tcacggtgcc tgcaaacgga gcaggaactt
    30301 ccatagaagc cttatcgcct tctacggtga tcagcgactg ttcagcttca actttatcgc
    30361 caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc ggaacgttaa
    30421 cgtcttttgc cgccgcagcc gctggtgctg ctgccggagc tgcttctttc ttctcttctg
    30481 cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata atcagtgcgc
    30541 cggtctgggt tttatcgcca acagagactt tgatctcttt aacgataccc gcctgcggag
    30601 atggtacttc catagaggct ttgtcgcctt ctacggtgat cagcgactgt tcggcttcaa
    30661 ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc ccgatgtccg
    30721 gtactttgat ttcgatagcc attattcttt tacctcttaa gccagacgcg ggttaacttt
    30781 atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat cgatttcgcc
    30841 acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa cttcgaagtg
    30901 gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca gtacgcggta
    30961 gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat agtcggtaga
    31021 tgccactgcc ggagcgtcgt tcatcacctg agcgatgtat ggaacgcgcg gagtttccag
    31081 cgggtgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagct cggtgaagga
    31141 ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct ctgccgcttc
    31201 acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt tgctaccttc
    31261 aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt ccggcattgc
    31321 cggcatgtgg tagttttcgt tcagcgtagt gatgtagtag taaacgttct cttgtttttc
    31381 accgtacata cgctccagac cgtcatgcat gatgacagca acttcgtaag cgtaagccgg
    31441 gtcgtaagag atacagttcg ggatagtcag cgactgaatg tggctgtgac catcttcgtg
    31501 ctgcagacct tcgccgttca gggtggtacg accggaagta ccgccgatca ggaagccacg
    31561 cgcttgctgg tcgccagccg cccagcacag atcgccgata cgctggaagc cgaacatcga
    31621 gtaatagatg tagaacggaa tcatcggcag attgttggtg ctgtaagagg tcgccgctgc
    31681 cagccaggaa caccctgcgc ccagctcgtt gatcccttcc tgcagaatct gacctttctc
    31741 gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct gaccgttcgg
    31801 gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg cttcgtcggc
    31861 gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt tcagggcacg
    31921 aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg cgccgaagtc
    31981 ttgcaggctc ggcagctcaa gcttctcggt gaagttcggc tgacggcttg gcagataacc
    32041 gtgcagtttc tgacgctgag cgtgcagata ggtatgctct tcagaacctt ccgggaaggt
    32101 gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac ggtcgcggat
    32161 atgacgcacg ccgtccatgt tcattttctt aacctggtgc gcgatgtttt taccttcagc
    32221 cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attaccgtcg ctttgccttt
    32281 ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac cgccacggtt
    32341 cagcgcccag atctgctcgt cagtccagtc tgcaaccaga gctgcggttt ccggatattt
    32401 accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct ggtagtcgcc
    32461 gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac gcagcagttc
    32521 atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt cgaagatgcc
    32581 ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac gctgcaggtt
    32641 acagttgata acgaagacca ggttatccag tttttcacgg gtagcgatgg tgatcgcacc
    32701 tttggattcc ggttcgtcca tttcaccgtc gccgaggaag gcgtaaacgg tctgtttaga
    32761 ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt aaatagcacc
    32821 aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca tcagtttcgg
    32881 gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat ccagctgctc
    32941 ctgagtcaga cgaccttcca ggaaagcacg agcgtaaacg cccggggaga tgtggccctg
    33001 gaagtaaacc agatcgccgc catcctgctc gttgcgtgca cggaagaagt gattaaagca
    33061 cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca gttcgaggtc
    33121 ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag cggatagctg aacgaatacg
    33181 gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga tggtgttgat
    33241 gtagttgctg ataccggtgc ctgcggctac gttgacaccg cctttgcggg cttcagcaag
    33301 cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga ccgattcgat
    33361 cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac gttctgacat
    33421 gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc attgaactct
    33481 cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta tatgcgcttg
    33541 atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt cgttgctcca
    33601 gacgacgcag agaacgctca cggcggctct cttcacggct tctgtcgagc aaaatttctt
    33661 cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta ccggccataa
    33721 tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg cgacgcgaat
    33781 agagcaattc gaagttctgg cggacattct gggccaacat cggctccata caccttagca
    33841 gatgaagcag aaccacattg tgggccgctt cagtgacagc aatctgatac tggagtacgg
    33901 cgtttgattc cgcgtccaga tcgccagact gctgcgccag ctctatggcg tgatggagtt
    33961 cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa gcggcgatac
    34021 cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatga tcggagagca
    34081 gctctaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa gtgccgccac
    34141 cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca cgcaaggagg
    34201 gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt tcgcccgggc
    34261 ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg gagagttttg
    34321 gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc gaagtcaatt
    34381 ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct tatgtccata
    34441 caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat atataacgtt
    34501 tcaaagttgt aactatgcac aaatgtagac tttacgtagg aaagggattt tttaacgatt
    34561 attaaactat aaaaatccga attgaaccga ttcacttacc aatttatcga tttttaattc
    34621 aaaaataatg aattta
//
LOCUS       DARNVM010000032        34220 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.032, whole genome shotgun sequence.
ACCESSION   DARNVM010000032 DARNVM010000000
VERSION     DARNVM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.032"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1806)
                     /gene="gss"
                     /locus_tag="SI621_001439"
     CDS             complement(<1..1806)
                     /gene="gss"
                     /locus_tag="SI621_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HEI4064171.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDES"
     gene            2011..2877
                     /gene="yghU"
                     /locus_tag="SI621_001440"
     CDS             2011..2877
                     /gene="yghU"
                     /locus_tag="SI621_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEI4064172.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2947..3195)
                     /gene="hybG"
                     /locus_tag="SI621_001441"
     CDS             complement(2947..3195)
                     /gene="hybG"
                     /locus_tag="SI621_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HEI4064173.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3208..3549)
                     /gene="hypA"
                     /locus_tag="SI621_001442"
     CDS             complement(3208..3549)
                     /gene="hypA"
                     /locus_tag="SI621_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEI4064174.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEENAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3542..4030)
                     /gene="hybE"
                     /locus_tag="SI621_001443"
     CDS             complement(3542..4030)
                     /gene="hybE"
                     /locus_tag="SI621_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HEI4064175.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4023..4517)
                     /locus_tag="SI621_001444"
     CDS             complement(4023..4517)
                     /locus_tag="SI621_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HEI4064176.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4517..6220)
                     /gene="hybC"
                     /locus_tag="SI621_001445"
     CDS             complement(4517..6220)
                     /gene="hybC"
                     /locus_tag="SI621_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HEI4064177.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6217..7395)
                     /gene="hybB"
                     /locus_tag="SI621_001446"
     CDS             complement(6217..7395)
                     /gene="hybB"
                     /locus_tag="SI621_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEI4064178.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7385..8371)
                     /gene="hybA"
                     /locus_tag="SI621_001447"
     CDS             complement(7385..8371)
                     /gene="hybA"
                     /locus_tag="SI621_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEI4064179.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8374..9492)
                     /gene="hybO"
                     /locus_tag="SI621_001448"
     CDS             complement(8374..9492)
                     /gene="hybO"
                     /locus_tag="SI621_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HEI4064180.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9681..9968)
                     /locus_tag="SI621_001449"
     CDS             complement(9681..9968)
                     /locus_tag="SI621_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HEI4064181.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10087..10974)
                     /locus_tag="SI621_001450"
     CDS             complement(10087..10974)
                     /locus_tag="SI621_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEI4064182.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11131..12171
                     /gene="gpr"
                     /locus_tag="SI621_001451"
     CDS             11131..12171
                     /gene="gpr"
                     /locus_tag="SI621_001451"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEI4064183.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAVYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQHNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12211..12705)
                     /locus_tag="SI621_001452"
     CDS             complement(12211..12705)
                     /locus_tag="SI621_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEI4064184.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            12896..13780
                     /gene="yghA"
                     /locus_tag="SI621_001453"
     CDS             12896..13780
                     /gene="yghA"
                     /locus_tag="SI621_001453"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HEI4064185.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEQDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLATQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13819..14244)
                     /gene="exbD"
                     /locus_tag="SI621_001454"
     CDS             complement(13819..14244)
                     /gene="exbD"
                     /locus_tag="SI621_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEI4064186.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14251..14985)
                     /gene="exbB"
                     /locus_tag="SI621_001455"
     CDS             complement(14251..14985)
                     /gene="exbB"
                     /locus_tag="SI621_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEI4064187.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15237..16424
                     /gene="metC"
                     /locus_tag="SI621_001456"
     CDS             15237..16424
                     /gene="metC"
                     /locus_tag="SI621_001456"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEI4064188.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVAAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHVQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAGHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDKFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16564..17223
                     /locus_tag="SI621_001457"
     CDS             16564..17223
                     /locus_tag="SI621_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4064189.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTILMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17263..18162)
                     /gene="yqhC"
                     /locus_tag="SI621_001458"
     CDS             complement(17263..18162)
                     /gene="yqhC"
                     /locus_tag="SI621_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HEI4064190.1"
                     /translation="MKREEICRLLADKVNTLKNKENGLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18356..19519
                     /gene="yqhD"
                     /locus_tag="SI621_001459"
     CDS             18356..19519
                     /gene="yqhD"
                     /locus_tag="SI621_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEI4064191.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19624..20451
                     /gene="dkgA"
                     /locus_tag="SI621_001460"
     CDS             19624..20451
                     /gene="dkgA"
                     /locus_tag="SI621_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HEI4064192.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20651..21577
                     /locus_tag="SI621_001461"
     CDS             20651..21577
                     /locus_tag="SI621_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HEI4064193.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELVEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QVERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21628..21885
                     /gene="yqhH"
                     /locus_tag="SI621_001462"
     CDS             21628..21885
                     /gene="yqhH"
                     /locus_tag="SI621_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HEI4064194.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(21927..24146)
                     /locus_tag="SI621_001463"
     CDS             complement(21927..24146)
                     /locus_tag="SI621_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEI4064195.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGVKKRPKAAVGR"
     gene            complement(24257..25669)
                     /gene="ftsP"
                     /locus_tag="SI621_001464"
     CDS             complement(24257..25669)
                     /gene="ftsP"
                     /locus_tag="SI621_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HEI4064196.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25744..26481)
                     /gene="plsC"
                     /locus_tag="SI621_001465"
                     /gene_synonym="parF"
     CDS             complement(25744..26481)
                     /gene="plsC"
                     /locus_tag="SI621_001465"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HEI4064197.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIADLDKEVAEREAAGKV"
     gene            complement(26715..28973)
                     /gene="parC"
                     /locus_tag="SI621_001466"
     CDS             complement(26715..28973)
                     /gene="parC"
                     /locus_tag="SI621_001466"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEI4064198.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29111..30718)
                     /locus_tag="SI621_001467"
     CDS             complement(29111..30718)
                     /locus_tag="SI621_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEI4064199.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLEQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQISAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRTGDFMLSRQSWDATYNDASTFLNTLKS
                     DSEENVGHWKNAQYDGLLNQAAQTADATKRNALYQQAEVIINQQAPLIPVYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKTH"
     gene            complement(30827..31309)
                     /locus_tag="SI621_001468"
     CDS             complement(30827..31309)
                     /locus_tag="SI621_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEI4064200.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(31362..31754)
                     /gene="ygiW"
                     /locus_tag="SI621_001469"
     CDS             complement(31362..31754)
                     /gene="ygiW"
                     /locus_tag="SI621_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HEI4064201.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31906..32565
                     /gene="qseB"
                     /locus_tag="SI621_001470"
     CDS             31906..32565
                     /gene="qseB"
                     /locus_tag="SI621_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HEI4064202.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32562..33911
                     /gene="qseC"
                     /locus_tag="SI621_001471"
     CDS             32562..33911
                     /gene="qseC"
                     /locus_tag="SI621_001471"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HEI4064203.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNAENFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33960..>34220)
                     /locus_tag="SI621_001472"
     CDS             complement(33960..>34220)
                     /locus_tag="SI621_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HEI4064204.1"
                     /translation="DAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWLYVFTLVICLVCGWWLFWGRFIFC"
BASE COUNT         8339 a         9157 c         8680 g         8044 t
ORIGIN      
        1 tgattcatca ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac
       61 ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg
      121 ctcggcaaat ttaccgctgg ttttgtccag cacctcttca tgatggctga cgaggtcgat
      181 gttactgcca cagcgaccgg cgatcggttt cactgcgtaa cctgttttta ccagttcatc
      241 attaacagtg aaatcggtat ccagtaggta acgatggtgc gggaacagcg accagaggat
      301 cggcagaatc gctttgttgc cggggatcac tgtccacaga ggttcaaaga ccagcacttc
      361 cgggcgcagc aatacgtcga taagacgcac ttcattttgc ggatgaccgg tacggatcgg
      421 caccgcagca aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc
      481 ccaggttttc cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc
      541 atcccagccc agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag
      601 cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata tctttgtctt gcatgatatg
      661 gacaaacgga cgtgcacgac tgtgtttcca ggcaccggcc agttcgttaa tcagcccttc
      721 cgccggattg aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc
      781 cgcttcggta tgacaggagg cggaatcggc gttgtattcg tagaccttca ggccacgttc
      841 gtccatacag aaatccatac gaccggtgat catatggtga cggcgacgct gccaggagag
      901 acgcaaacgt ggccagagga tttttgggat gtcgaacagc gccagcaggt tgtcatcttt
      961 cagcaccttg tcggtggcgt gaagatacat caggtgcagc tcgttggtgg ctttaatcag
     1021 ctcctgctcg gcactctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg
     1081 accgttggcc tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc
     1141 aaactggcct ttgttttcca ggcgcgctcc gctgattttc agcaactcgc ctgcaatttc
     1201 cggctgcggt aagctgtatt cagtatcttc cgtctggatc atccagccga gaatggtggt
     1261 gtcatcaaac gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg
     1321 cgtccactgt tgcccttgcg gtaacgggga atgaatcacg ttctgttccg caatacggac
     1381 tttgttgcca tgcaattggg taatgatggc gacatggcca gtgtctttaa attcaccgcc
     1441 tttatcccag ataagaagcg cgcccgcgac cggtgcacgc ggcgagccat taggaaatgc
     1501 ctgcaatggc aggatgttgt cattaaccac ttcacgcagg aagcgcagcg agaaaatctc
     1561 ccacgccata cccacgtcag taaagaccac accgtaattc agaaagagaa aacggcgagc
     1621 aaattcaacg cattgccatt tgtggcccat atattcgtcg tcgatatagc tacggaatac
     1681 ggcgtcatct tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac
     1741 accacctggg gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt
     1801 gctcatcact ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga
     1861 ttacctgctc agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc
     1921 ggctggcgca aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct
     1981 tcaacactac cactcagaag gcaactcact atgacagaca atacttatca gcccgcgaaa
     2041 gtctggacgt gggataaatc cgctggcggc gcgttcgcca atatcaatcg cccggtttct
     2101 ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacatc cattgcaact ttattcactg
     2161 ggaacgccga acggtcagaa agtaacgatt atgcttgagg agctgctggc gctgggcgtt
     2221 acgggtgcag agtacgacgc ctggctgata cgcataggcg atggcgatca attctccagc
     2281 ggttttgtcg aagtaaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat
     2341 ccgccgatcc gcgtgtttga gtctggatcg attctgcttt atctggcgga gaaatttggc
     2401 tacttcctgc cgcaggatct ggcaaagcgt actgaagcaa tgaactggtt gttctggtta
     2461 cagggtgctg cgccgtttct cggcggtggt tttggtcact tttaccatta cgcgccggtg
     2521 aaaattgagt acgccatcaa tcgctttacc atggaagcca agcgcctgct cgacgtgctg
     2581 gataagcaac tggcgcagca taagtttgtt gcgggcgatg agtacaccat tgcggatatg
     2641 gcgatttggc cgtggtttgg caacgtggtg ttaggcggcg tgtatgatgc cgcagagttt
     2701 cttgatgcag gtagttataa acacgtacaa cgctgggcga aagaagtggg cgaacgtccg
     2761 gcggtgaagc gtgggcgtat tgttaaccgc accaacggac cgctgaacga gcagttgcat
     2821 gaacgccatg acgccagtga tttcgagacg aatacggaag ataagcgtca ggggtaagtc
     2881 gcaacattgg caggcttatc gctatgtagc cggataaagc atttacgccg catccggctt
     2941 taatcatcac gcgctggtaa tgtcgtaatc catttggcgc agcgcgtcta atgtggcttt
     3001 ggcttcatct tcgtcgatga tgctcatggc aaatccgacg tgtaccagca cccactggcc
     3061 cagcagatcg gcagggttac cttcacaaat cagggcgata ttcacgtcgc gcttgatacc
     3121 acatacttca acctgcgcaa gctggtgaat atcctcaccg acagccagca cctggcctgg
     3181 aacgccaata cacattgtta actccggtta ttcaacttca atacttttga cgatcagcga
     3241 atcgccggta tcgacacgca accgctcgcc atgacagagc ggacactgcg catcgtgctg
     3301 atgaatctcc accacctggc tgcaatccca gcaccaggcc tgggcgggtt tatagacgat
     3361 atgtaaatcg cacccttgcg ccaccgttcc gtggcagaca atttcaaaac taaaacggac
     3421 ggcgttctcc tcaacgcagg agagcgcgcc aatttccagc cacacggcgg tgacgcgctt
     3481 aacatcgtgc tgctccgcct gccgttggat aatttcaacg gcgctctggc aaagcgacaa
     3541 ctcatgcatt ttcgccactc ctgcggccaa acagcagggc gcgacgccct gcgtgtggta
     3601 catccggatt cgtgactggc agcgaaagga tcattcgtgc gcagtcatcc gtcaggcgtt
     3661 ggccctcttc aatcgacatg ctgtgcgaaa gcggcgacat cagcgagcag gagaggtatt
     3721 gcgaaacacc gtccagttcg ccaacggtaa aggtcatggt gccatacggc agttgcagac
     3781 caattttttc actcactttg cgcagcggcc agagttgatc cgggccgggg aagataactg
     3841 cactcagcat ccacggggtg atcacacacc ccgtccactg accttcgaac agcgtaaagt
     3901 cagaaacata caccggcatt gaaggatgca gaaaagagag atcatgcatc gaacgccggg
     3961 caatttcttc aaaagctgcc tgtacttgcg ccttcgggga ggtctggaaa cctgctatct
     4021 cttcagtcat gagtcgcctc ccgtgggatg gcttccacgc ctgattcgcg cagcgcagcc
     4081 agaacctgct caagcgcagg ttcaatcatt gcttcaaccg tcggcgttaa gccgatgtgt
     4141 ggctccagcg attccgggat cacgccgacc agggtcagct ttttcggaaa ctcaccggtg
     4201 aagcgcaggg ccgacaatac gtcggccagg ccaagctgat gcggagagat tttgttggta
     4261 aacaacgccg gaacttcttc atcccgcagg atcatcatcg ttcccggcgc gctcttcttc
     4321 gacacaatgg catccgcaat gatcagatga tcgcgatttg ccatgtcgcc aagcagctcc
     4381 attcccgccg tgccgccatc gaggatctca acataatccg gcagaatgta tcgttgctct
     4441 aacgcttcga caatccgcac accgatggct tcatcggtca gcaaaatatt gccaacccct
     4501 aagactaaaa tacgcattac agaaccttca ctgaaaccac ttcgttgccg tcggcatcca
     4561 ctacgtgtac cgcacaggcc atgcacgggt cgaaggagtg aatggtacgc accacttcca
     4621 gcggtttatt cggatcggca accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt
     4681 catcattgaa gttacgtggg ccagagttcc aggttgatgg aacaactgcc tggtagttgc
     4741 tgatgatacc gtctttaata accatccagt gagagagcat accgcgcggt gcttcgagga
     4801 agccaacacc tttgaactca cccgttgccg gaatgttcgg cttcacaaag gtggtgtgat
     4861 cgcctttgcc gatattggtg atcagtgcac tgtattgatt ttgcaggata tcctgcaatt
     4921 cgcagcagtg aacggtacga ccaataatac ggcccagcgt ggagtgcagc tgcgccactt
     4981 ccagcgtgtt gccagtcagt ttctgataaa tcgcaacgat ttcattcagt ttattctggg
     5041 tagattcgcg acctgccgcc agtttcacca gcatattagc cagcggaccc acttctaccg
     5101 ttttgccgta gaaagtcggt gatttcaccc aggaatattt cccgtcgtca gaccaaccat
     5161 cataagccgg aatggtggtg ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg
     5221 agtgcttcgc gctttcctga atgcctttga tcaggtattc atcggaatga gaagtgatcg
     5281 gacgatacga ggacagatcc gcattctcaa tgtagccgcc cgggaacagg aagctgccgt
     5341 ttttactgtc ggtcgggaat tccggcacgc tcaggtagtt caccgcacct ttaccgcgcg
     5401 tcagccattc cggatagaac gcggcgataa ccgcggtatc aaccttataa acctgctcaa
     5461 caaagtcgct cagcttgtcg atgaaggact tgatgtacat cagacgctca agattcagca
     5521 cgcccagacc gtcgaggttg atcgggttag cgaccccacc tactgccagg ttctgaatgt
     5581 gcggcgtttt accgcccagc agcgccacga cgcggttagc gtcacgctgg cactccagcg
     5641 cctgcaggta gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc atcgccgggt
     5701 gaccccagta gccattggcg aaaataccca actgaccgct ggcaaccaga tctttgatct
     5761 tgttctgaac tttagtgaac tcttccggac tgttcagatg ccaggtcgaa acgcctttca
     5821 gcatttcgga ggctttggtt gggtcagctt gcagtgcaga agtgatatcc acccagtcca
     5881 gcgccgaaag ttgatagaaa tgaacgatat ggtcatgcgt ggtgtgcgca gccagaatga
     5941 tgttacggat gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactc tctgccgcac
     6001 gaacggaaga cagcgcgtga gtggtagtac atacgccaca gatacgttgc acaatcatcc
     6061 atgcatcgcg cggatcgcgg tttttcacga tctcttccat gccgcgccac atggtaccgg
     6121 aagcccatgc tttcgaaacg acaccatttt cgatttcgca atcgatgcgt aaatgcccct
     6181 caatacgggt taccggatca atagtaattc tctggctcat gctttgctcg cctcatgacg
     6241 attatgatcg ttttgtttta aaggaggaag tatcggcagt agacgaatga gtacgatgta
     6301 agcgcaaatc tcaatagcca caaaaccaat agaaatcaac agttcttccc aggtcgggaa
     6361 gtaggcgtaa ccgccgcccg ggttgaatgc caccagcgaa taggtcagac gccaggttgc
     6421 acaacctaac agtgcgctca gtgctgacag gaacagcatg cgggaatcat tacgcagctt
     6481 cgccacacgc agaacgacca gcgggaagag catcagcagg acttcaatcc agaacatcac
     6541 ggagtagaag tcaccggcaa acgctaacga cagcttgtcg cgatagataa gctcgccaaa
     6601 gcgcagcacg atgaaaatcg ccagcaacac actgatggtg ttggtcagct taacgaacag
     6661 actcttttca tccggaccgt tgccacgcag acccgcctgc accagcgaac cttcaaagat
     6721 gacaatcgag aagcccatga tgaacgccgt cagcagtgag aacagcggca acatttcata
     6781 gctctgccac agcggatgca ctttgtagcc cgccgagatc atcagcgacc ccattgaaga
     6841 ctggtgcata gttggcagca gcgcaccgag cgcgatgatg aagaacatca ccttgttcag
     6901 ccgtttgagc gacactttcc agcccagacg ttcaaacagt gccggagcaa actccagtgc
     6961 catcacgccg atgtagatgg tcatacagac cgccgtctcg aacagtaccg agttcacgtt
     7021 gaagtgaccc ggaatgtaga agtacggcag gttccagtag cgaccgacgt cgatagtgat
     7081 cgacaagcca cccagtgagt aaccgaacag actcgccaac agcgccggac gcactagcgg
     7141 atggtattgt ccacggttaa agacgtatac cgcccacgcc agcgcccagc cgccacaggc
     7201 gaagccggtg ccgatcagca ggtcaaacgc gatccatacg ccccacggga aaccgccgtt
     7261 caggtcagag acagagccca gaccgaacac cagacgcttc acaatcagga gcatacagat
     7321 gacgattaac ggtccaaaaa tcatgaccgg tttactgatg attttgccgc ccagcggttg
     7381 tggatcatga ctcatgatcg tctcctccgt cgtgatggtc gtttttggtg ttgcgacgaa
     7441 ccagcacggt taagcccgcc agcacggcca gtggtagcat catgccttta tacagggtgt
     7501 gttgaatatt ttcggaacgc gcaccggtag aaagatcgtc cagcttcggc aggtcgagat
     7561 tttcataagg cacacccgtc agtaccagaa cctgagtacc gccgccctct ttctcgccgt
     7621 acagatgcgg ataatatttc ggcaccgtgt gcaggtaagt gtcgccagat ttcagcgtct
     7681 gacgcggata gtggtattcg ctgccaggct tcagcgccag gcgttttttc gcctccgcca
     7741 tcagctcttc acgcgtacca aaaatcaccg cgcccgccgg gcacacttct acgcagccag
     7801 gcagaccgcc tttatcaaga cgttccacac ctttctggtt gcacagttca cacttatgca
     7861 gcgcaccaaa tgggttgttg tagtcgtact tcggcacgtt gtatgggcag gcgaccatgc
     7921 agtaacggca gccagtgcac acatctttgt cgtaatggac aatgccggtt ttcggatctt
     7981 ttttcagcgc agagaccgga cacacggaga cacagttcgg atcgacgcag tgcatacact
     8041 gtttcttaat gtacgcgtag ccgttctctt cctggtcttt gttgacaccc gtgccactgg
     8101 tccacacctg aatgatgtta ttggtatacg gcgacagttt gtcgttgttc gaccaggtct
     8161 gttccccttg cgggttacgt tcagggaagt tgatatcctg acacttggtg acgcaagcct
     8221 ggcagcctac gcacaaggtc gagtcataca acatccccag cgatcccgga attggcgggc
     8281 ggttttcagc agccgcatga ctgacagacg gcaacgcgcc cgtcagcaat gccccgcagg
     8341 aggctgcttt aataaaatta cgtctgttca cggttattct ccccgtgagt cagcgttatc
     8401 tttcttttgc tgacgcccca gttcacgcac cgccatcacg ctgacaccgg caaccagccc
     8461 aaccacgccg ccgagcaaac caatagcgcc cgcagagacg ttgccgcctt ctttagcgtt
     8521 gacgtccggt ttttgtgaac gcggcgtctg gttttcgacg ttggcaagct gatggatgcc
     8581 tttatggaag ccgatacctt cttcgttaca gccatagcat gggtgaccaa ttgccaccgg
     8641 ccacacaccg ccaacgtcgc agaattgcag cgttgagcag ttgccgtaag tttctggccc
     8701 tttacagccg aggtggtaca ggcaccaacc ttcgcggtgg ccttcatcgc cgaactcttt
     8761 ggcaaaacga ccagcatcga agtgcggacg acgttcacag tgttcgtgaa tcagacgacc
     8821 ataggcgaag gtcggacggt ttttatcatc cagcttcggc ggtttgccgt aagtgatgat
     8881 gtgcgcaacg gtcgcgagga agttgtgcgg gtttggcggg cagcctggaa tgttaataac
     8941 ggttttgcct ggcagaactt cttgcaggct gaccgcgccg gtagggttaa ccccagccgc
     9001 ggcaacaccg ccccacgcag agcaggaacc gatagcgata atggctgctg cgccttccgc
     9061 cgctttgcgg atgtgatcca caatcggctc accggcaacc atgcaataaa taccattatc
     9121 ttttaatggg atggaaccat ccaccactaa cacatactgc cctttgtact tctcgagagc
     9181 gttatgtttg ttctcttcga cctgatgacc gaaggcggcg gaaagcactt cgtgatactc
     9241 cagagagata gtctccagca cgaggttttc taccgttgga tgcgttgcac gaagcagaga
     9301 ttccgtacaa ccggtacact cctgcgcgcc aatccagata actggcggac gctgcgggtt
     9361 agtaaccgat tcggccatct ctgcagcggc tttgctactt aaccccatgg tggcggctaa
     9421 tgctgcacaa agcttcatga aatcacgacg gttaatgccg tgagaatgga tgagggtgtt
     9481 atctccagtc atttatagtt attccgttgc gaagacctgg catatatttt gcctcaatcg
     9541 caaaatcaat aatgcgatcg atgcgccatt taccacacat ttattatggt tatcgatatc
     9601 atgatactgc ggcggggcgc taaaacgaag ggaagaagca ttagtgtaat taattaaagc
     9661 aagataatac gtatgtttga tcaattttcg aggcgataac cggccataaa aaagcgcaac
     9721 gtagaacagg aattattctc acgaaagaaa tccattacca tctctttatc cagtccatag
     9781 cggcgcgtca gaatacccgc ttcccaggcg ctaagctggc gatctccggt cttttcgtac
     9841 atccggtgtg agtagcctaa tacaaaaccg cgtttataat cggcacagta ttttgtcaca
     9901 ttaaccgcac tgtcggcatg cgttgctttt aatcccgcca ttaagccttt accaaaatgg
     9961 ttattcatcg ttcacctcat tcgctatata ttgccatata tagcgatatt ttaggcaaag
    10021 aacgactttt gcgcacgcta ttttttcatg cttgtttaag acgtaatccc gtaagagata
    10081 aacctatcag gagagatatt tatcgaacca tttcagcgtc ctttgccagg caagatcggc
    10141 ggcagatttg tcataacggg gcgtggaatc attatggaat ccgtgattaa cccccggata
    10201 gatatacgcc tcatagacct tattattggc tttcaacgcc gcctcgtaag caggccagcc
    10261 ctcgttgatt cgggtgtcca gttccgcgta gtggagtagt aaaggcgctt caatcttcgc
    10321 cacatcggcg gtgggtgcct gtcgaccata aaacggcacc gcgcaggcca gttccggata
    10381 cgcgacagcc gccgcgttcg atacgccacc gccatagcaa aatccggtaa tacccacttt
    10441 gcctgtcgct tgcggatagc gttgcataaa ctcaattgcg gcaaagaaat cattcatcag
    10501 tttggttgga tcaacctgct gttgcagctc acgaccttta tcatcattac ccggataacc
    10561 tccaacggaa cttaagccgt caggtgccag ggcgatatac cccgccttcg ccactcgccg
    10621 tgccacatct tcgatatacg gattcagtcc acgattctca tgcaccacca ccacggctgg
    10681 cgttttgccg ctcatctttg cgggcttcac cagataaccc cgtacctcgc cgtgaccatt
    10741 tggtgaagga tacgtgatgt actcagcaac aatctccggg tcggtaaact ctacctgagt
    10801 cgctagcgca taatttggct tgagcaaatc aaacaacgct aatgccgtca tcccgcctac
    10861 cgcatacttc gccgcaaggt tgaggaactc acgtttcgag attttcccgt gagcgtaata
    10921 gtcgtagtaa tccagcaact cttgtgggaa atctttggcg gttaaacgcg gcatcattgc
    10981 actcctcagc tggtgttttt ttaagcaaag cataagcacg tgtttttgcc cagtttttcg
    11041 tcactctgtg agccagacta cgggatacgc gctgatgaat cgctaaacta gaaacattgt
    11101 ttcgaaattg aatggtggaa aggagaggtc atggtctggt tagcgaatcc cgagcgttac
    11161 gggcagatgc agtaccgtta ttgcggaaaa agcggtttac gcctgcccgc gttatcgctc
    11221 ggtttatggc acaatttcgg tcacgttaac gcgctggaat cacagcgtgc gatcctgcgt
    11281 aaagcgtttg atttaggcat tacgcacttt gatttagcca acaattacgg gccgcctcca
    11341 ggaagcgcag aagagaactt tggtcgcctg cttcgggagg attttgctgt ttatcgcgat
    11401 gaactgatta tctctaccaa agccggatac gatatgtggc ccggcccgta cggctctggc
    11461 ggctcacgta aatacctgct cgccagcctc gaccaaagcc tgaagcggat ggggcttgag
    11521 tatgtcgata tcttttactc tcatcgcgtc gatgaaaata cgccgatgga agaaaccgcc
    11581 tctgcgctgg ctcatgcggt acaaagcggt aaggcgcttt atgtcgggat ctcctcttac
    11641 tcgccagagc ggacgcaaaa aatggtcgag ttgctgcgcg agtggaaaat tccgctgtta
    11701 attcatcaac cttcttacaa tttactgaac cgctgggtgg ataaaagcgg cctgctggat
    11761 accctgcaac ataacggcgt gggctgcatt gcctttactc ctctggctca gggattgctg
    11821 accggaaaat atctcaacgg cattcctgaa gattcacgga tgcatcgtga agggaataaa
    11881 gttcgtggtc tgacgccgaa aatgctcacc gaagccaacc tcaacagcct gcggttattg
    11941 aatgaaatgg cacagcagcg tggacaatca atggcgcaaa tggcgttaag ctggttgctg
    12001 aaagatgatc gcgtgacgtc ggtattgatt ggtgccagcc gcgcggagca acttgaggag
    12061 aacgtgcagg cgctgaataa tctgacattt agcaccgagg agctggcgca gattgatcag
    12121 catatcgccg atggcgagct gaatctgtgg caggcgtctt ccgataaatg acctgttaat
    12181 aacgggcgcg agaaccgcgc ccgaatcaga tcagtgatta tgacgagtca gtcggtcaag
    12241 atagcccatc acaaatgcag agagcacaaa cgtcagatgg ataatgacgt accacatcag
    12301 tttgttatca gggacatttt tcgcatccat aaagacgcgc agtaagtgaa tggaagaaat
    12361 tgccacaatc gacgctgcta ctttgttttt cagcgacgtt gcgtccattt tccccagcca
    12421 gttcagcttc tctttgttct cggagatatc cagctgcgag acgaaattct cataaccgga
    12481 aaacatcacc atcaccagca aaccgccaac cagcgtcata tccaccagcg acagcaacac
    12541 gaggatcaaa tctgattccg ccatcgagaa gatattcggc agtacgtgaa taatctcctg
    12601 gaagaacttc agcgccaggg caactaacgc cagcgaaagg ccaaagtaca cgggggcaag
    12661 cagccagcga gaagcataca ttgcattttc aagaaaacgt tccatacatt cctgtcgtgt
    12721 agttaaaacg ggcaacagta tatcgcaatt gagtaaacag cgcgcaacag caaatcaaca
    12781 tatccgttaa acctttgtcg cataaatccg aggtttaggg atcttcctgg ttcccttatc
    12841 aacgcatcga gagcgtctac ccttaaagga cgttgaacct gaagggagaa aaacgatgtc
    12901 tcatttaaaa gacccgacca cgcagtatta cactggtgaa tatcccaaac agaaacaacc
    12961 gacgccaggc atccaggcga aaatgacacc ggtgccggat tgcggcgaga aaacctatgt
    13021 tggtagcggt cgcctgaaag atcgtaaagc actggtgaca gggggcgatt ccggaatcgg
    13081 tcgcgctgcc gccattgctt acgcgcgtga aggggctgac gtggcgatca gttatcttcc
    13141 cgtagaagaa caagacgctc aggatgtgaa aaagatcatt gaagaatgcg gacgcaaagc
    13201 cgtcctgctg ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg ttcacgaagc
    13261 gcataaggcg ttaggcgggc tggatattat ggcgctggtc gccgggaaac aggttgccat
    13321 tcctgatatt gcagacctca ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt
    13381 cgcgctgttc tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg ccagtatcat
    13441 caccacttcg tcaatccagg cataccagcc aagcccgcat ttactggact atgcggctac
    13501 gaaggcggcg attctgaact acagccgtgg cctggcaaca caggtcgcag agaaaggtat
    13561 tcgggtgaat attgttgcgc caggcccgat ctggacagca ctgcaaattt ccggcgggca
    13621 aacgcaggat aagatcccgc agtttggtca gcaaacgccg atgaaacgtg cggggcaacc
    13681 ggcggaactg gcccctgtat atgtttatct ggcaagtcag gagtcgagct acgtcaccgc
    13741 agaagtgcac ggcgtgtgcg gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa
    13801 agcaaccggg catcattttt atttcgcttt ggcggtttct tcgccgacca gacctatctt
    13861 cagataaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt
    13921 tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc
    13981 cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt
    14041 caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa
    14101 gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac
    14161 caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag
    14221 gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg
    14281 aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac
    14341 ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgtg caaatacgtt
    14401 atagataacg accgccggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc
    14461 tgcaataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa
    14521 gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggcgaaa tcgcgccgat
    14581 ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga cccgacgctc
    14641 cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc
    14701 cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc
    14761 ggcaatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct gctcgcgctt
    14821 aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac
    14881 tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata
    14941 cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt atctccagga
    15001 cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcgttct
    15061 cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa
    15121 aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc
    15181 ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgacatgg
    15241 cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactctcg
    15301 gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt ctttgacagt gtggcagcca
    15361 aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt
    15421 taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgtgc
    15481 tgttcccctg cggggcggcg gcggttgcta attctattct tgcttttgtc gaacagggcg
    15541 atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc
    15601 tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggcgca gatatcgtta
    15661 aacatgtgca gccaaacact aaaatcgtgt ttctggaatc cccaggctcc atcaccatgg
    15721 aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg gatgccatca
    15781 ttatgatcga taacacctgg gcggccggtg tgctgtttaa ggcgctggat tttggcatcg
    15841 atgtttccat tcaggccgct accaaatatc tggttgggca ttcagatgcg atgattggta
    15901 ctgccgtatg caatgcccgt tgctgggagc agctgcggga gaatgcctat ctgatgggcc
    15961 agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcact ttgggtgttc
    16021 gcctgcgtca acatcatgaa agcagtctga aagtggctga gtggctggca ggacatccgc
    16081 aagtagcgcg agttaaccac cctgctctgc ctggcagtaa aggccacgaa ttctggaaac
    16141 gagactttac aggtagcagc gggctatttt cctttgtgct taagaaaaaa ctcaatgatg
    16201 aagagctggc gaactatctg gataaattca gtttattcag catggcctac tcgtggggtg
    16261 ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt cgcccacaag
    16321 gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg
    16381 atctgattgc cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga
    16441 caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt
    16501 cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag
    16561 tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg
    16621 gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa
    16681 aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt ggctggcgca
    16741 ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac cgccgcagca
    16801 agtctgggct gctggttaag ttatattcag gggcgctggt tagggaatac caaaacggtg
    16861 aaaggctggc tggcacagct tcccgctaaa tatcaccagc gcgccacctg catgtttgac
    16921 cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg tacgttgctg
    16981 ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta
    17041 agtggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag tatgattccg
    17101 ttcgttaaac gccatgaaga tcaggtaatg acgatcctga tgatcctgcc aattgccttg
    17161 ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc
    17221 tgacgatttt ccccgttccc ggttgctgta ccgggaacgt atttaattcc cctgcatcgc
    17281 ccgcattctt gccgcatctt cccccggcgt cacaccaaag taacgtttaa actcacggct
    17341 aaattgcgat gcgctttcgt aaccgacgcg catcgctgcc gcactggctt tcatgccgtc
    17401 gtggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga
    17461 ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc
    17521 cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg
    17581 gctaatcagg ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg
    17641 tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc
    17701 cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa
    17761 cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat
    17821 gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg
    17881 cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta
    17941 ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa
    18001 gagaattatg atgccaggct cgtacattac cggtgtacgt gcgaaaggcg tctcgccata
    18061 caacaaacgc acatcgggca acagctctga caaaccattt tctttatttt tcagtgtatt
    18121 aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg
    18181 aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa
    18241 taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa
    18301 aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa
    18361 caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt
    18421 acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggcggcg gcagcgtgaa
    18481 aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt
    18541 tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg
    18601 cgaacaaaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa
    18661 atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac
    18721 gggcggtaaa gaaattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac
    18781 cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc
    18841 attccattct gcccatgttc aacccgtatt tgccgtgctc gatccggttt atacctacac
    18901 tctgccgccg cgtcaggtgg ctaacggtgt agtggatgcc tttgtgcaca ccgtggaaca
    18961 gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgtt
    19021 gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc
    19081 caacgttatg tgggcggcga ctcaggcgct gaacggtttg atcggcgctg gcgtaccgca
    19141 ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc
    19201 gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc
    19261 taagctgctg caatatgctg aacgcgtctg gaacatcaca gaaggttccg acgatgagcg
    19321 tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca
    19381 cctctccgac tacggtctgg acggcagctc catcccggct ttgttgaaaa aactggaaga
    19441 gcacggtatg acccaactgg gcgaaaatca tgacattacg ctggatgtca gccgccgcat
    19501 atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc
    19561 agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa
    19621 cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga
    19681 ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa
    19741 gtgggttatc gctcgattga taccgccgcg gcctataaga acgaagaagg tgtcggcaaa
    19801 gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac
    19861 gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat
    19921 tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca
    19981 tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac
    20041 ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgttattaat
    20101 cagatcgaac ttcacccgct gatgcaacaa cgccagctac atgcctggaa cgcgacacac
    20161 aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag
    20221 aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat tgttatccgc
    20281 tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc acgcattgtc
    20341 gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga aattgcaaaa
    20401 ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt ttggcggcta acgtgcaaat
    20461 tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc
    20521 gtcctgccca ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc
    20581 agagccggaa gctcttaaag gaacaattca tctacacccg ggataaacgc aacggagagg
    20641 tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca
    20701 cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg cagatttatc agaactataa
    20761 atcagatgcc agtacgcctt atttcggtga aaccggagag cgggcgataa cttcagcgcg
    20821 tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct
    20881 ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgttag aaagcgtagc
    20941 gataacccaa cctgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga
    21001 cgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat
    21061 tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa
    21121 aacgctggcc gctatagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact
    21181 cgttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg
    21241 ccaggcggtc aatgccttct ataaaaccac cttcaagact gccaacaatc ccgatgaaga
    21301 tatgcaagta gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgtt
    21361 attttcccgc gttgatgaaa ttcgagtcct ggagaaaacc accgattccg cccgcattca
    21421 tgttcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt tacagcggcg
    21481 cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca
    21541 gattgaggca aaaaccgccg ccagattaaa acaatgagct gagttaaata acaattagcc
    21601 ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt
    21661 ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc
    21721 aatgtgcaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcattacgc
    21781 ccacaaatct atgctgccaa atccgacgct aacagagcca atacgcgtct tgatgctcag
    21841 gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaattcccgg
    21901 tagcattctg ttaccgggga tattttttaa cgtccaaccg ccgctttagg gcgtttcttc
    21961 acaccagcat tcaccggacg agattgcgta gacgacgctt tttttgccgt agcaggcgtc
    22021 tgacgctggg tcgccatcgg cgtatgttta gtcaacgccg gacgggtatt gcggttctgg
    22081 cgacgagcct cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg
    22141 ccaatcagat gctttttgcc catcgcttct agcgcctggc ggattaacgg ccagtttgcc
    22201 ggatcgtggt aacgcaacaa cgctttatgc aggcgacgct gcttgtcgcc cttcggtaca
    22261 aagacgtctt cacttttata accaatcttc gccagcgggt ttttcccggt gtaatacatg
    22321 gtggttgagt tcgccagcgg cgacgggtag aagttctgta cctggtcgag gcggaagcgg
    22381 tgctttttca gccacagcgc cagattcacc atatcttcat cgcgcgtacc ggggtgtgcc
    22441 gagatgaaat aggggatcaa atactgctct ttacctgcct gtttcgagta ggtatcgaac
    22501 aactctttaa agcggtcata gctgcccatg cccggcttca tcatcttcga caacggccct
    22561 tcttcggtat gttccggggc aatcttcaga taaccgccga cgtgatgggt cgccagttct
    22621 ttgatatagc gcggatcttc cacggcgatg tcataacgca caccagaggc aatcaggatc
    22681 tttttaatgc ctttcagatc acgcgcacgg cgatagaggt tgatcgtcgg ttcgtggtta
    22741 gtgtccatgt gcggacaaat atccggataa acgcacgaca aacgacggca ggtttgctca
    22801 gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc gagatcggaa
    22861 attacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa
    22921 tcttccgaac ggctctgaat aatgcgccct tcatgctcgg tgatagaaca gaaagagcag
    22981 ccgccaaagc agccacgcat aatgttgacc gagaaacgga tcatttcgta agccggaata
    23041 cgagcattgc cataggccgg atgcggcacg cgcttgtacg gcagcgcaaa aacgctgtcc
    23101 atctcttcgg tagagagcgg aatagcaggc gggttgatcc acacatagcg gtcgccatgt
    23161 ttttgcatca atgcgcgggc gcagcctggg ttagtttcgt ggtgcagaat acgcgaagca
    23221 tgggcgtaca gcactttatc gcccttcact ttctcgaaag aaggcagcaa tacgtaggtt
    23281 ttttcccacg gtttcgggcg cggcggctgc acggttacgg ctttcgcttc ctgctttttc
    23341 ggcgcaacag gtttgttatc cgcgcacggc aaatcttcac catacggatg cgggattggg
    23401 tcgatttttc caggggtatc aagacgggtg gaatccacgc cgctccagcc aggcagcgcc
    23461 tctttcacga taatcgcggt attacgcaca tcgcggattt cactaatcgg ctcgcccatc
    23521 gccagacggt gcgccacctc caccagcgga cgctcaccgt taccaaacat cagcatgtcg
    23581 gctttcgaat ccaccagcac ggaacggcgc acggtatcgg accagtaatc ataatgtgcg
    23641 gtacggcgca gactagcctc aataccgccg aggatcaccg gtacatcttt ccacgcctct
    23701 ttacaacgct gggtataaac cagtgtggcg cgatccgggc gcttacccgc gacgttatcc
    23761 ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg tataacggtt gatcatcgaa
    23821 tccatgttgc cagcagtaac accgaaaaac agattcggtt tacccagacg cataaagtcg
    23881 tctttgctgc tccagtccgg ttgggcaatg atcccgacac gaaagccctg cgcttccagc
    23941 atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc gccagtaacc
    24001 aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga catcggcaaa
    24061 aacggtgccg gtccaaaaca ggcggcccag tactgcggcc aggagaacag gtcgcgatcc
    24121 ggttggatca gggagataga gctcattttg cttccagaaa tgataaaaaa ataatcaaag
    24181 gccggggatt ataagccgga acgaaagaga aatcgaaagg tattccatac tcgccctcct
    24241 cgggcgagta tgaagattac ggtaccggat tgaccaacag ttgccctatc gagccacggt
    24301 ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac
    24361 cgaaataaac cagcagctcc acttgtccgt caacccaaac ggtatctttc cagcctctgt
    24421 cttccgggaa cggcatcgcg ccattcacgt tacggatctg gaacatcacg ccttcaatat
    24481 ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg
    24541 tgacatcaat acggttgacg tcccacagtt gtccattaat acctgggtca tcacccagac
    24601 tgatatcgcg actacgaatc ggcgaaccgg ccatgatttc agttggcagc aagcgcatcg
    24661 gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc accagggtag
    24721 aaaccagaat gctggatggc tcaaagaagc cacgaatacg atcaacaatg ctcgccgctt
    24781 cgccacaggt gatcgacact tcatcgccgt tgctcatatc caccagaatc tcgcggcgct
    24841 cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc aggcaggaat ccctgatcgc
    24901 cagaaatcac atgtaacggg cgaccatcgc tcatctgcag ttgatagcga cgagagttcg
    24961 acgcgttcag cagtcgcaag cgcacccagc ctcgcgaaac ttcaacgtac gggctttgta
    25021 caccgttaac cagcagtgtg tcaccaacaa agccgccgct tcccggttcg ttgtattctg
    25081 gcgtaccaaa gttgtccagc cgtttatcct ggataatgac cggaaaatca tccacaccat
    25141 aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac attccggcaa
    25201 ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac cacagagtag
    25261 ctgcgttctg gcgaataggc agtacgggtg cccagtcagc gtttggcgac atcatccgtg
    25321 ccggaccgcc catcagcggg ccaggtacct gtagcccggc caccgtcatt gagacatttt
    25381 ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg cggatagtcg
    25441 gccccaggta acgaccattg attccccaaa ccgacgcgcg tgtccctggc gtaaatgacc
    25501 agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca agcagcggcg
    25561 gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca gcgcctgcac
    25621 aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata tttgctccat
    25681 gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat acagattgcc
    25741 gacttaaact tttccggcgg cttcgcgctc tgcgacttct ttatcgagat cagcgatttt
    25801 ttgttccatt atcgaacgac aatgggcagc caactcgcga acctgatctt tgccatactg
    25861 actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgta gccgattaag
    25921 attaatctta ttcgaagttg tagagacgca cacgggaata atcgggacgc ccgccgcaat
    25981 tgccgcgtga aatgccccgg tcttgaacgg tagcaggccg cgaccacggc tgcgggttcc
    26041 ttccgggaac atccagatgg aaatacggcg ttttttgaag tgattcacga cttccgcaat
    26101 agtgccgtga gctttagtgc gattgtttct gtcgatcaat aggttgccgg ttaaccagta
    26161 caactgcccg aagaagggga tccacagcaa gctctttttc cccaccgtca ccgtcggcgg
    26221 ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag cgatatagat
    26281 agcattgccg tagctttcag cgtctgcagg tttgcggcac tccactttca ggccgaaaag
    26341 cggcgcaaga cggccaaaca tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct
    26401 gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca cggtaataat
    26461 aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc attgtacgtc
    26521 acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc
    26581 gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg ggttgaatac
    26641 gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacagcg
    26701 ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc
    26761 tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca
    26821 gtgactttct gtagctcttc cggacgcagt ttaattttgc gtttcccaac atgaatggtc
    26881 agcgtgcttt gcggcggcag aacgtacagt tgcgccagac catcttctcc acgcgcggct
    26941 tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg
    27001 cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat atcagaagca
    27061 tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcttta
    27121 cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg
    27181 gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgcccca
    27241 ggcggcaacg ttaatttgcc ggtgagcggt tcgccctgac cacgcgccga cggcagcgta
    27301 atcgggtcaa tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc
    27361 ttacctttca ccgccgcttt gaagctatcg cccgctttat aattcaggcc cggcgcgtcg
    27421 atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt
    27481 tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc
    27541 gaacgacgtt cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta
    27601 ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagctca
    27661 ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg
    27721 aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt
    27781 tcatcttcat tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc caccagcaaa
    27841 ccttcgagga tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc
    27901 actgtatcgc ggcggaacac cagccattcg gagagaattt ccagcaggtt tttcaccgcc
    27961 ggacggccat ccagaccgat catattgagg ttaatgcgat agctcttttc cagatcggtg
    28021 gttgcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc
    28081 accagacggg tcggattctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc
    28141 tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc
    28201 ggcaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact
    28261 gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat aatttccgct
    28321 tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc
    28381 tggtcgatta atgcgattgc cgcctgagcc acttcacgca ggttatgcgg tggaatatcg
    28441 gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgggca
    28501 ggtagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc
    28561 tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc
    28621 attgctgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc
    28681 ggataacggt aagagaacgg ctgcgccatc agaaccatcg cttcataaca ggcactatcg
    28741 ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg
    28801 gcgctggcat tcaggcccag ctcagacatc gcatacacaa tgcggcgctg aacaggtttc
    28861 agaccatcgc caataaacgg caacgcgcgg tccatgatca cgtacatgga gtagtttaaa
    28921 taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct
    28981 gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat
    29041 ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc
    29101 gataaagaca tcaatgtgtc ttgatataca actctttgct gtagacataa tcctgaggat
    29161 tatgcagcgg aaaaccgcca acgtagggtt taagcagttt gattaacggc tgatagtaga
    29221 caggaatgag cggtgcctgc tggttgatga tcacttctgc ctgctgatac agcgcattac
    29281 gctttgttgc atcagccgtt tgtgccgcct ggtttagtaa gccgtcatac tgcgcgtttt
    29341 tccagtggcc gacattttcc tcgctatcgc tcttaagcgt gttcagaaag gtggaggcat
    29401 cattgtacgt cgcatcccac gactgccgcg acagcatgaa atcaccggtt cgtcgggcat
    29461 caagataggt tttccactcc attgtgcgca gcgtcacctg tgcacccagc cattttttcc
    29521 attcggaaga caacgctatc gcggtttttt catgcagatc gtacttgttg tagaacaact
    29581 caaagcgtag cggatgagag gcgtcgtatc ccgcctgttt cagcaaggct tttgccatcg
    29641 cgacacgctc actcattggc ttttgcagtt catcgaacgt cgtcgcgcta aagcctttta
    29701 cctctggcgg cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt tgcgcaataa
    29761 gctgtcgatc aaccgtaaga tatagcgccc gacgcacccg cacatcgtta aatggcggtt
    29821 tctcaaggtt aaagttgtaa tattcgctgt tcagacgcgg gataattcgt agctcgccag
    29881 gcagtgattt ttcaatggca gaaatttgct gcgccggaac ccaggtgaga tcgacctctc
    29941 ccgcgcgata gcggttatag ccggtgaccg aattatccag cgccagatac tcaacctgtt
    30001 gcaatactgt atgttgcgca tcgcggtact ttggattttt gcgtgcagta atcttttcgt
    30061 taactaccca ctgttcaagc acaaaggcac cgttgtaaac catgttctct ggcttactcc
    30121 agctatcgcc atgtttagcg atgacatgat gaggaaccgg gaacagcgtc ggccaggcga
    30181 gcatcgtcgt gaaccaagga accggctgct caagcgtaac ttcaagagta cgatcatccg
    30241 tcgctttgac acccagcgat gtaacatctg ctttacccgc aacaatagct gcggcattgt
    30301 taatgtgcgc ctgtgccaga tagccagcaa aagggcttgc cgttttcggg tcaaccgcgc
    30361 gctgccagcc gaggacaaaa tcctctgccg tcagaggctg accgtcggac cactgcaaac
    30421 cgctacgcag atggaaaata tagcgcttgc cgccgtccag tatctcccag cgttcagcct
    30481 gagcgggctg cacctggcct tcaccgtcca tccataccag accttcaaac agatccagca
    30541 caatctgcgc ggcagtattc tcctccacct tttgcgggtc gagcgtacct gggtcgctat
    30601 gattgttgta acgaaagact tgttgcgggg cgagcggtgt gttggcggga acgtcagcag
    30661 cgtagagagg agccgcactt accaggctga ccagccataa taaatttcgc gtatacatgg
    30721 tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat tgttcgctca
    30781 aacttatcgc gagtgatttg gctcacactc cggtaaagat tcttcatcac gccaacggca
    30841 cataaacatc tgtttccagt cgctgctctg tcatcccttc ggcaagattg gtgtagtgaa
    30901 acagaatcgg tgctttacgc attttctcgc cgcttgcagg tagccagtgg cgaatgatgc
    30961 cccatatcgt gtgtgaaata tcgtccagct cgccaacatg gcgggccaca gcataacgtc
    31021 caccggtaag ttcaccatta ctcacaccat aacgattatc ggggatcggt tcgctaacgc
    31081 tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtca tcccaggcga
    31141 cgccaaaagt ctggctttgg tttacaggag aaagccccgt ctctttacgc cacatgataa
    31201 atttcgccac gctgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag
    31261 ggattgatgg gaaatcgata atatttacat ccagtgtcag gtttgtcatt ttgcctccct
    31321 gcgaaattat tcccgggaac gtgtcccggg agcggtaacg attacggatt tactttgcgg
    31381 atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca
    31441 accgtatctt tcggcgtcac cgtcacgccg ttccagcgtt tgtggtcgat atcaacattg
    31501 atagtaccgc tggcatcttt gaacacgtag agatcgtcag agatgcgttc aacgatattg
    31561 ccgcgtaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg
    31621 ctgccattcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg gccagaaaaa
    31681 ccgccctgct ctgctgccat taccggtgcg ctgcacaggg ccattactgc gattactgct
    31741 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
    31801 gtccttaaca acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca
    31861 acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
    31921 atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa aatgggtttt
    31981 agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctatatag tgcgccttat
    32041 gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa
    32101 tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga tgcgttagcg
    32161 gaacgtgtag aggggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
    32221 atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca ggccagcaac
    32281 gaactgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc
    32341 gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
    32401 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
    32461 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt
    32521 attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
    32581 ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcagtg acctggctgc
    32641 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgagttg ttcgacaccc
    32701 aactgatgct gtttgccaag cggttaagta cgctcgatct taacgaaatc aacgcggcag
    32761 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga
    32821 cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat aacggagaag
    32881 atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg
    32941 atccctggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggtc
    33001 aggaatggga ataccgcgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt
    33061 ggcttgtcgc actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg
    33121 cgccgctgaa caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac
    33181 taaacgcgac gggcgtaccc agcgaagtgc gtccactggt tgagtcgttg aatcaactgt
    33241 tcgcccgcac acatgcgatg atggttcgtg aacgacgttt tacctccgac gcagctcacg
    33301 aactacgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg
    33361 atgatccgca ggcgcggaaa aaagcgctgc ttcaattaca ttccggaatc gatcgtgcta
    33421 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    33481 acgtcgcgga gatcccgctt gaagatctcc tgcaatcatc ggtgatggat atttaccaca
    33541 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc atcaaacgca
    33601 ccggacaacc gctgttgcta agtttgttgg tacgaaacct tctggataac gccgtgcgct
    33661 acagtccaca gggcagcgtg gtggacgtca cgctgaatgc cgaaaatttc atcgtgaggg
    33721 ataacggccc cggtgtgacg ccagaggcac tggcgagaat tggcgaacgc ttctatcgcc
    33781 cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca
    33841 aattgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaagg
    33901 tgagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag attttactat
    33961 tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccaaaca aataacgagc
    34021 gtgaatacat atagccaaaa ctcgaacatt tcccgcataa agagttttct taagataaga
    34081 ataataagtg gcgtcagaag aaaaaatgct gcaatcattg cttgcaatat acgatcttcg
    34141 cctgaaacgg aaggtatact gcaaatattg tctcccgact caagcatcca gtcatactta
    34201 ttgaaagcaa aaaaagcatc
//
LOCUS       DARNVM010000033        33535 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.033, whole genome shotgun sequence.
ACCESSION   DARNVM010000033 DARNVM010000000
VERSION     DARNVM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.033"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            160..3027
                     /locus_tag="SI621_001473"
     CDS             160..3027
                     /locus_tag="SI621_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4064205.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKYGAIGLNAIIFSPLL
                     IAADLGSQNGTTITISDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAVANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSSGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSFWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HTSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGNGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3127..5046)
                     /gene="dhaR"
                     /locus_tag="SI621_001474"
     CDS             complement(3127..5046)
                     /gene="dhaR"
                     /locus_tag="SI621_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HEI4064206.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGHLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5274..6344
                     /gene="dhaK"
                     /locus_tag="SI621_001475"
     CDS             5274..6344
                     /gene="dhaK"
                     /locus_tag="SI621_001475"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEI4064207.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6355..6987
                     /gene="dhaL"
                     /locus_tag="SI621_001476"
     CDS             6355..6987
                     /gene="dhaL"
                     /locus_tag="SI621_001476"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HEI4064208.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            6998..8416
                     /gene="dhaM"
                     /locus_tag="SI621_001477"
     CDS             6998..8416
                     /gene="dhaM"
                     /locus_tag="SI621_001477"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HEI4064209.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TEDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            8735..10432
                     /gene="treA"
                     /locus_tag="SI621_001478"
     CDS             8735..10432
                     /gene="treA"
                     /locus_tag="SI621_001478"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HEI4064210.1"
                     /translation="MKSPTPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAIANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            10770..11792
                     /locus_tag="SI621_001479"
     CDS             10770..11792
                     /locus_tag="SI621_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEI4064211.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDVPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            11792..12772
                     /locus_tag="SI621_001480"
     CDS             11792..12772
                     /locus_tag="SI621_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEI4064212.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDIQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            12769..13527
                     /locus_tag="SI621_001481"
     CDS             12769..13527
                     /locus_tag="SI621_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4064213.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAIKH"
     gene            13537..14349
                     /locus_tag="SI621_001482"
     CDS             13537..14349
                     /locus_tag="SI621_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEI4064214.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLKVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAMASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVQPEALR"
     gene            14346..15200
                     /locus_tag="SI621_001483"
     CDS             14346..15200
                     /locus_tag="SI621_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HEI4064215.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            15226..17196
                     /locus_tag="SI621_001484"
     CDS             15226..17196
                     /locus_tag="SI621_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEI4064216.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQVKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(17246..17500)
                     /locus_tag="SI621_001485"
     CDS             complement(17246..17500)
                     /locus_tag="SI621_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEI4064217.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            17701..18435
                     /locus_tag="SI621_001486"
     CDS             17701..18435
                     /locus_tag="SI621_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEI4064218.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(18437..19048)
                     /gene="emtA"
                     /locus_tag="SI621_001487"
     CDS             complement(18437..19048)
                     /gene="emtA"
                     /locus_tag="SI621_001487"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HEI4064219.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            19148..20062
                     /gene="ldcA"
                     /locus_tag="SI621_001488"
     CDS             19148..20062
                     /gene="ldcA"
                     /locus_tag="SI621_001488"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HEI4064220.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVDVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEPRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            20157..21893
                     /locus_tag="SI621_001489"
     CDS             20157..21893
                     /locus_tag="SI621_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEI4064221.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(21995..22282)
                     /locus_tag="SI621_001490"
     CDS             complement(21995..22282)
                     /locus_tag="SI621_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064222.1"
                     /translation="MHPVLQADETPLQLLSSLKRVLCGLRQCGRLRGGICSGDVRLGP
                     LMVDGDTGNAGLVILHLLQSEEAIMLTFLSRRFELISQSLQWFVIHNLTFF"
     gene            22543..23979
                     /locus_tag="SI621_001491"
     CDS             22543..23979
                     /locus_tag="SI621_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064223.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMK
                     YIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
                     RAEALRRMKECLITRQSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLKCYENCIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISAPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLFKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEKLEDLDDLMDLELLDDPVLLERVKVQEDLELLNQQLGLLILERQQDSQPVNQHSE
                     HEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(24147..25217)
                     /gene="dadX"
                     /locus_tag="SI621_001492"
     CDS             complement(24147..25217)
                     /gene="dadX"
                     /locus_tag="SI621_001492"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HEI4064224.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMERIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIIAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVMTV"
     gene            complement(25227..26525)
                     /gene="dadA"
                     /locus_tag="SI621_001493"
     CDS             complement(25227..26525)
                     /gene="dadA"
                     /locus_tag="SI621_001493"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEI4064225.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            26855..28387
                     /locus_tag="SI621_001494"
     CDS             26855..28387
                     /locus_tag="SI621_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEI4064226.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(28437..29156)
                     /gene="fadR"
                     /locus_tag="SI621_001495"
     CDS             complement(28437..29156)
                     /gene="fadR"
                     /locus_tag="SI621_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HEI4064227.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            29378..30919
                     /gene="nhaB"
                     /locus_tag="SI621_001496"
     CDS             29378..30919
                     /gene="nhaB"
                     /locus_tag="SI621_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HEI4064228.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            31065..31595
                     /gene="dsbB"
                     /locus_tag="SI621_001497"
     CDS             31065..31595
                     /gene="dsbB"
                     /locus_tag="SI621_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HEI4064229.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLLVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(31641..32909)
                     /gene="umuC"
                     /locus_tag="SI621_001498"
     CDS             complement(31641..32909)
                     /gene="umuC"
                     /locus_tag="SI621_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HEI4064230.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLAGTDIRFIRKHF
                     NVVLERTVRELHGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPPPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(32909..33328)
                     /locus_tag="SI621_001499"
     CDS             complement(32909..33328)
                     /locus_tag="SI621_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEI4064231.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         8262 a         8678 c         8394 g         8201 t
ORIGIN      
        1 tgtgttcctg attttgcccg gtaatagaga attattctca gagcagctca tcatgattgc
       61 accatacaaa tatcatgatg ataagaggat attattcatt gacttaaaca aaatgacaaa
      121 atccatagac ccatagcctg ggtactatat aggagtggta tgggcatcaa acaacacaat
      181 gggaatacca aagccgatcg tctcgctgaa ttaaaaatcc gttcgccctc cattcaactg
      241 ataaaatatg gcgctattgg tttgaatgca attatctttt cccccctgct gatagctgct
      301 gatttaggaa gtcaaaatgg caccactatt actattagtg atggtgacag aattacagga
      361 gataccgccg atccatcagg aaacctctat ggtgtaatga ccccagcagg aaacacgcct
      421 ggcaatatca acctgggtaa tgatgtcacc gtcaatgtca acgacgcctc tggatatgca
      481 aagggaatca ttattcaggg caagaacagc tccctgacag ctaaccgact cacagtagat
      541 gttgttggtc aaacctctgc catcggcatt aatttaattg gtgactatac ccatgctgac
      601 ttaggcacag gcagcaccat taagagtaac gatgacggca tcattattgg acatagctca
      661 acactaacag ccactcaatt caccattgaa aactcgaacg gtataggcct aaccatcaat
      721 gactatggca ccagtgtcga tcttggaagc ggaagtaaaa tcaagaccga tggaagtaca
      781 ggtgtttata tcggtggtct caacggcaat aacgccaatg gtgctgcgcg ttttacggcg
      841 acagacctga caatcgatgt tcagggctac agcgccatgg ggataaacgt acagaaaaac
      901 tctgttgtcg atctcggaac aaacagtacc attaaaacca atggcgataa tgcacacggc
      961 ctctggagct ttggccaggt gagcgcgaat gcactcactg ttgatgtaac tggagccgtg
     1021 gcaaatggcg tcgaagttcg tggtggtaca accactatcg gtgctgatag ccatatttct
     1081 tccgcgcagg gcggtggcct cgtaaccagt ggttcagacg cgacaatcaa tttttctggc
     1141 acggcagcgc aacgaaacag catcttttcc agcggttctt atggtgcctc agcccagacg
     1201 gcaacggctg ttatcaacat gcaaaatacc gatattacgg ttgatcgtaa tggcagtctg
     1261 gcgctgggtt tgtgggcgct cagcggcggt agaataaccg gagacagttt ggctatcacc
     1321 ggcgcggcag gagccagggg gatttatgcc atgaccaaca gccagatcga tctcacgagc
     1381 gatctggtca ttgatatgag tacacccgac cagatggcca tcgcaacgca acatgacgat
     1441 ggttatgccg ccagccgcat caacgcttcg ggtcgtatgc ttatcaacgg tagcgttctc
     1501 tccaaaggtg ggctaatcaa tctggatatg caccctgggt cgttttggac aggttcttcc
     1561 ctcagcgata atgtcaatgg cgggaaactg gacgttgcaa tgaataacag cgtctggaac
     1621 gtaacaagta attctaatct cgacacgctg gcgctgagcc attcaactgt cgattttgcc
     1681 agccacacgt caactgccgg cacatttacc acattaaacg tagagaacct gagcggtaac
     1741 agtaccttta ttatgcgtgc tgatgttgtt ggcgagggta atggcgttaa taataaaggg
     1801 gatttattga atatcagcgg gagtagtgct ggtaatcacg tattggctat ccgtaaccag
     1861 ggcagcgagg ccacaacggg aaatgaagtt ctgacagtgg taaaaaccac tgacggcgcg
     1921 gcctcgttca gcgcgtcttc tcaggttgag ttgggaggat atctgtatga tgtgcgtaaa
     1981 aatggcacta actgggagct ttatgcttcc gggacaattc cggagccgac tcctaatcct
     2041 gagcccacac cagctcccgc tcagcccccc atagtcaacc ccgatcctac gcctaacccc
     2101 actcccacac ctaaacccac cacgaccgca gatgctggcg gcaattatct caatgtcggt
     2161 tacttattga actatgttga aaaccgtacg ctgatgcaac ggatgggtga cctgcgaaat
     2221 cagagtaaag acggaaatat ctggctgcgc agttatgggg gaagtctgga ctccttcgcc
     2281 agtggcaaac tgagcggctt tgacatgggt tacagcggta tccagtttgg tggggataaa
     2341 cgtctctctg atgtaatgcc gttgtatgtc ggtctgtata ttggctcaac acatgcatcg
     2401 ccggactata gcggaggcaa cggtaccgca cgttcagact acatgggaat gtacgccagt
     2461 tacatggcac aaaacggttt ttacagcgat ctcgttataa aagcatcgcg ccagaaaaat
     2521 agtttccacg tactggacag tcagaacaac ggcgttaacg ccaacggcac tgcgaatgga
     2581 atgagtatct ctctggaagc cgggcagagg ttcaacctgt cccctactgg ttatgggttc
     2641 tatatagagc cgcaaaccca gctcacatac agccaccaga atgagatggc tatgaaggcg
     2701 agtaatggcc tcaatataca tctgaatcac tacgaatcgc tgctggggcg tgccagcatg
     2761 atactgggat atgacatcac cgcaggcaac agccagctga atgtctatgt gaagactggc
     2821 gctatccgcg agttttcagg ggataccgaa tatctgttga acaactcccg ggagaagtac
     2881 agtttcaaag gtaatggctg gaataacggc gtgggagtca gtgcacagta taacaaacag
     2941 cacacattct atctcgaagc ggattacacg cagggtaacc tctttgatca gaagcaagtc
     3001 aacggaggat atcgcttcag cttttaagtg ggggatagac aagcccttaa acgatgctga
     3061 aaattgttag cgtgcgccgg aatgaaatac agatcactct gatgtatcat aaatcgtttc
     3121 tgtctttcat acccggcgct taaactgccc tgcatcaatg ccatgttgct tcattttccg
     3181 ccacagcgta gtgcggccga ttccaagtaa agccgacatt tcctgaatgc gaccgcctgt
     3241 gacctgggct gcgttaataa ttgcctcttt ttcaacttcc gcaaatgaca gactggtgga
     3301 aaggcgggtg gcgctgacat catctgtcgc ctgctcggta aacagatgtt caggtaaatc
     3361 actgacgcga atgcgcccgt tatcactact cagagcaaga ttctcgatga cgctgtaaag
     3421 ttcaaaatcg ttgccaggcc atgcacagga aaccaggcga gcgagggcat cgtcatcaat
     3481 tttcagccgc gtagagaagc gtttttcaag actgtgtaat ttgttattca ccagcgccgg
     3541 aatgctgcca cgccgcatac gcagaggcgg gatggtaatt tcaaatgcat gcagcgcgta
     3601 atacagctgg cgactaaagc gattttgttc caccagcatt gcgaggtccg cggtcgttgt
     3661 ggcaatcact ttgacatcaa tgggtattaa acgccgcgca tccagtcgcg tgataacacc
     3721 ctgcttgata acctgaagca aagcagactg taactccacc gccagatatt caatcttttc
     3781 aagaaacagc gtgccgccgt gcgccagttc cagccgactc agacggccat tttcattatc
     3841 cgtgcgatcg ccaccaataa attcttccgc cagcgccgca tcaccatata actcacaatt
     3901 gacggcaata taaggccccg ctgcacgctc gctttcatta tgaattgcct gacttagcag
     3961 tgccttgccc acgccctcct ctccacaaag caggacagga aagctactgc gcgccgcctg
     4021 gcgaccaaaa tgaatcaagc ggcgggtttg cggatcctct tgtggcatat gagcgaaagt
     4081 atggctgact tttcctaatt gactggtcat taactgccgc atctgttcca caggatggag
     4141 caacaaaata aagctggttc cctgcgtttc tattatcggt ttaagggtta tcaccgcatc
     4201 aataaactga tgctgacttt caaaggttgc ttctacgtgt ttgagcggat gtgcctgttt
     4261 tattgcttgt tgcaatacgg cgggtaacgt taagagttca gtgatcgccc ttccctgact
     4321 tgccgtcgcg tcaaggcgca agacccgcgc cgcctgggca ttgataaatt gcaaatgacc
     4381 ctgctcgtcc cagctaatca caccatcatc catactttct aacagggcat taagttgatt
     4441 taaatgacgg ttagtttcag cgagcaaact gtccgtcagc agtaaatttc cgacctcgcg
     4501 tgcgattgcc aacgtcaacg gcaaatcagc tgcggtagtt tgctcaaccg gacacgccag
     4561 cgctattgtt cccgtcaatc ggcccttgct gtcaaacaac ggcgttgcac aaaaggccca
     4621 gttccagagt gcctgtttga aatgttgatc ggccatcgtt ttcacggcct gaccagagat
     4681 agccgctaac gatagcgcac aagtaccaat aattccctcg gcgcaatacg tgccgtcatt
     4741 gaatcccagt gtacttagtt gctgcaaggt ttgcggatcg ccattacggc tgagaatgca
     4801 ggcggtttca tcgaggataa gcaacgcaca ctctcgcggt gccatatatt cccaggcatc
     4861 ttccagcgca gcctgcccga gcgtcagcat cgctttctta cgccgataaa tagaagcaaa
     4921 tgtcacgccc tgggcctggt gtggtacgtt ccatgtctcc cgtttcatca gcttattgca
     4981 tctctcccag gaggttgcaa ttaagggaga tatgccccga ccatcgttgt taaaagcgcc
     5041 actcatatcc cgcatccctt atgacaaaag gtgttccggc tcgcaatttt aagtattaat
     5101 acacgtaaaa gtttcaatat ggaacattca ttaaagcata tgtttcacta tgaatcataa
     5161 tttgggctaa ttttcttttc ccttgcctga tgcacaatgg attcatcggc agtcagtggt
     5221 cgccgtgtcg ttgaacatca tccatgccct accgtaattg ctggagcaaa ataatgaaaa
     5281 aattgatcaa tgatgtgcaa gacgtactgg acgaacaact ggcaggacta gcgaaagcgc
     5341 atccatcgct gacactgcat caggatccgg tgtatgtcac ccgagctgat gcccccgttg
     5401 caggtaaagt cgccctgctg tcgggtggcg gcagcggaca cgagccgatg cattgtggtt
     5461 atatcggtca ggggatgctt tcgggggcct gtccgggcga aattttcacc tcaccgacgc
     5521 ccgataaaat ctttgaatgc gccatgcaaa ttgatggcgg cgaaggtgta ctgttgatta
     5581 tcaaaaatta caccggcgat attcttaact tcgaaacagc aaccgagtta ctgcacgata
     5641 gcggcgtaaa agtgaccact gtggtcattg atgacgacgt tgcagtaaaa gacagtcttt
     5701 ataccgccgg gcggcgcggc gttgccaaca ccgtattaat tgaaaaactc gtaggcgcag
     5761 cggcggagcg tggcgactca ctggacgcct gtgcggaact ggggcgtaag ttgaataatc
     5821 aaggccactc aataggtatt gctctcggtg cctgtaccgt tcctgccgcg ggcaaacctt
     5881 cttttaccct ggcggacaat gagatggagt ttggcgtcgg cattcatggt gagccgggta
     5941 ttgaccgacg ccccttctct tctcttgatc aaaccgtcga tgaaatgttc gacaccctgc
     6001 tggaaaatgg ctcataccat cgcactttgc gtttctggga ttatcaacaa ggcagctggc
     6061 aggaagaatc acaaaccaaa caaccgctcc agtctggcga tcgggtgatt gcgctggtta
     6121 acaatcttgg cgcaactccg ctttctgagc tgtacggcgt ctataaccgc ctgaccacac
     6181 gttgccagca agcgggattg actatcgagc gtaatttaat tggcgcgtac tgcacctcac
     6241 tggatatgac cggtttctca atcaccttac tgaaagttga tgacgaaacg ctggcactct
     6301 gggacgcccc ggtccacacc ccggccctta actggggtaa ataaggagat agcaatgtca
     6361 ctgagcagaa ctcaaattgt taactggctc actcgttgtg gcgatatttt cagcaccgag
     6421 agcgagtatc ttaccggact ggatcgcgaa attggcgacg ctgaccacgg gctaaatatg
     6481 aaccgaggct ttagcaaagt ggtggaaaaa ctccctgcta tcgcagataa agatatcggt
     6541 ttcattctca agaataccgg tatgacgcta ctttccagcg tcggtggtgc cagtggtccg
     6601 cttttcggta ccttctttat ccgcgccgca caggcgaccc aggcacggca aagcctgacg
     6661 ctggaagagc tttatcagat gttccgcgat ggcgcggacg gcgtaatcag tcgcggtaaa
     6721 gccgaaccag gcgataaaac catgtgtgat gtgtgggtgc cggtggtgga atcgttacgt
     6781 cagtccagcg agcaaaatct ctctgttccg gcggcgctcg aagctgccag tagcatcgcc
     6841 gagtccgctg cacaaagtac gattacgatg caagcccgca aaggccgcgc cagttatctc
     6901 ggtgaacgca gtattggtca ccaggacccc ggtgcgacct cggtgatgtt tatgatgcaa
     6961 atgttggcgt tagccgcaaa agagtaagga attggtgatg gtaaacctgg tcatagtttc
     7021 acatagcagc cgactgggag aaggtgtcgg tgaactagcc cgtcagatgt taatgagtga
     7081 tagttgtaaa atcgccattg ccgcgggaat tgacgatcca caaaatccca ttggtaccga
     7141 tgccgtcaaa gtgatggagg ccatcgaatc tgttgctgat gccgaccatg tgctggtcat
     7201 gatggatatg ggtagcgcat tattgagtgc tgaaactgcg ctggaattgc tggctcccga
     7261 gatcgccgca aaagtacgtt tgtgtgctgc accgttggtc gaaggtacac tggcagcaac
     7321 ggtcagcgcg gcctcggggg cggagatcga caaagttatc tttgacgcca tgcatgcgct
     7381 ggaagccaaa cgtgaacaac tgggtttacc gtcctccaac actgaaatct ctgacacatg
     7441 tcctccgtac gatgaagaag cccgttctct ggcggtggtc ataaaaaacc gtaacggcct
     7501 gcatgtacgt ccggcctccc ggctggttta taccttatcg acatttaatg ccgatatgtt
     7561 gctggagaaa aacggcaaat gcgtcacacc ggagagtatt aaccagattg cgttactaca
     7621 agttcgctat aacgatacgc tgcgcctgat tgcgaaaggg ccagaagctg aagaggcact
     7681 gatcgctttc cgtcagctgg ctgaagataa ctttggtgaa acggaggaag ttgctccacc
     7741 tattctgcgt cccgttccgc ctgtttcggg taaagccttt tattatcaac cggttttatg
     7801 tacggtacag gcaaaatcaa ccctgaccga ggatgaagaa caagaacgat tacgccaggc
     7861 cattgacttc acgttattag atctgatgac gttaacagcg aaagcagaag ccagcgggct
     7921 tgacgatatt gccgcaatct tttctggtca ccatacactg ttagatgatc cggaactgct
     7981 ggcggcggca agcgaactcc ttcagcatga acattgcacg gcagaatatg cctggcagca
     8041 agttcttaaa gaacttagcc agcaatacca gcaactggat gatgaatatc tacaggctcg
     8101 ctatattgat gtggacgatc ttctgcatcg caccctggtc cacctgaccc aaacgaaaga
     8161 agaactcccg cagtttaact cgccaactat tctactggcg gagaacatct atccctccac
     8221 aatactgcaa ctggatccgg cggttgtaaa aggtatctgc cttagcgccg gaagtccgct
     8281 atcccacagc gccctgatcg cccgtgaact ggggattggc tggatttgcc agcagggtga
     8341 gaaactgtat gcgatacaac cagaagaaac gctaacgctg gacgttaaaa cgcaacgttt
     8401 cagccgtcag ggttaaacca cagttcggat ggcatcgttc tgatgtcatc cggcaattta
     8461 ttctgcacgg atgtttttaa ctacctgtta tatttttcac caatttgtga tgatttttct
     8521 tcgctaatcg cgaacccaac gccgctggct ggctaacaaa cagacgttat ttgatcactc
     8581 tatttcgccg ttatttatcc ctttaattcc cttttctaaa atgcctgacc gttcgcagaa
     8641 tgagatttcg atcatgcagc tagtgcgatc ctgaactaag gttttctgat acttgaatac
     8701 cgttttattc ggtttcgcca aaggagaatg attgatgaaa tcccccaccc cttctcgccc
     8761 gcaaaaaatg gcgttaattc cagcctgcat ctttttgtgt tttgctgcgc tatcggtgca
     8821 ggcagaagaa acaccggtaa cgccacagcc gcctgatatt ttattagggc cgctgtttaa
     8881 tgatgtgcaa aacgccaaac tttttccgga ccaaaaaacc tttgctgatg ccgtgccgaa
     8941 cagcgatccg ctgatgatcc ttgctgatta tcggatgcag caaaaccaga gcggatttga
     9001 tctgcgccat ttcgttaacg tcaattttac cctgccgaaa gagggcgaga aatatgttcc
     9061 gccagagggg cagtcactgc gcgaacatat tgacggactt tggccggtat taacgcgttc
     9121 taccgaaaac accgaaaaat gggattctct gttaccgctg ccggaacctt atgtcgtgcc
     9181 aggcggacgt tttcgcgagg tatattactg ggacagttac ttcaccatgt taggacttgc
     9241 cgaaagcggt cactgggata aagtcgcgga tatggtggcc aattttgctc atgaaataga
     9301 cacttacggt catattccca acggcaaccg cagttactat ttaagtcgct cgcagccgcc
     9361 cttctttgcc ctgatggtag agttactggc gcagcatgaa ggcgatgccg cgttgaagca
     9421 atacctgccg caaatgcaaa aagaatatgc ttactggatg gacggtgttg aaaacctgca
     9481 agccggacaa caggaaaaac gcgttgtaaa acttcaggat ggtacccttc tcaaccgcta
     9541 ctgggacgat cgcgatacgc cacgaccaga gtcatgggtg gaagatattg ccaccgccaa
     9601 aagcaatccg aatcgacctg ccactgaaat ttaccgcgac ctgcgctctg ccgctgcgtc
     9661 tggctgggat ttcagctcgc gctggatgga caacccgcag cagttaaata ccttacgcac
     9721 caccagcatc gtaccggtcg atctgaacag cctgatgttt aaaatggaaa aaatcctcgc
     9781 ccgcgccagc aaagctgccg gagataacgc gatagcaaac cagtacgaaa cgctggcaaa
     9841 tgcccgtcaa aaagggatcg aaaaatacct gtggaacgat caacaaggct ggtatgccga
     9901 ttacgacctg aaaagtcata aagtgcgcaa tcagttaacc gcggccgccc tgttcccgct
     9961 gtacgtcaat gcggcagcga aagatcgcgc cagcaaaatg gcgacggcga cgaaaacaca
    10021 tctgctgcaa ccaggcggcc tgaacaccac gtcggtgaaa agtgggcaac aatgggatgc
    10081 gccaaacggc tgggcaccgt tgcagtgggt cgcgacagaa gggttacaaa actacgggca
    10141 aaaagaggtg gcgatggaca ttagctggca cttcctgacc aatgttcagc acacctatga
    10201 ccgggagaaa aagctggtgg aaaaatatga tgtcagcacc accggaacag ggggcggcgg
    10261 tggcgaatat ccattacagg atggctttgg ctggaccaat ggcgtgacgc tgaaaatgct
    10321 ggatttgatc tgcccgaaag agcaaccgtg tgacaatgtt ccggcgacgc gtccactcag
    10381 tgaatcaaca acacagccgg taaaacaaaa tgaggcggaa cctacgcctt aactttactg
    10441 tatatcgagc aatgccatta cgtgcataaa attggcattg ctctttcttc accacgaaat
    10501 accaaatacc actctttcta cggtctatgc ctttattttc ttttattaga gaagatttta
    10561 attttatttt atcaacttgt taatcattac acctctattt atattttccc ccactccgcc
    10621 attctccctg acaattttct tgtggaacaa agtgttattt aagaaacaag aaaatcattc
    10681 tgtctcttta gacaaatttt gacatcaaac atgatctttt tcgctatatg tgtaagtata
    10741 tagcggtttt aattatcagg atattactta tgtcactaac cagacggagg tttacacaga
    10801 ttcttgcgtc gacgttgttc ctgcatcatc tgccgtcctt tgcacaatca gtcaaattct
    10861 gggcctcact gacgcttccc gaagcccaaa acattacacg catcgtcagc gcaggcgcgc
    10921 ccgccgattt actattgctg gctgtcgcgc cagaaaaaat ggtcggtttt tcctcttttg
    10981 attttgcccg tcaggcatta attccattgc cagagcacat tcgccagttc cccaggctgg
    11041 gacgactcgc cgggcgcgcc agcacactct cgctggaagg gcttatggcg ttacatcccg
    11101 atttggtcgt tgattgcggc aatacggatg aaacctggat ctcccaggca cggcaggtta
    11161 gcgaacagac acaaataccc tggttattgc ttaacgggaa actggaacaa tcagcagaac
    11221 agttaacaac gcttggcaaa acgttaggcg aagagcaccg cgccgcagaa caagccaatc
    11281 tcgccagccg cttcgttggt gaagctcagg cattcgccac ctcacccgcc gctaacctca
    11341 gcttttatgc tgcgcgcggt cctcgagggc tggaaacggg cttacagggt tcgttgcata
    11401 ccgaggcggc ggaattatta ggtttgcata acgtcgcgca aatagccgat cgccacggtc
    11461 tgacacaggt ttccatggaa aatctcctgc gctggcagcc ggatattatt ctggttcagg
    11521 aggccgttac tgcagatttt attcgtcgtg atcctctctg gcagggcgtg aaagcggttg
    11581 cggaacaacg catcttattt ttaagtggcc tgccctttgg ctggctggat gtcccgccgg
    11641 gaatcaaccg tcttctggga ttacgcagac ttcacgcctg gctggatccc gccatcaatc
    11701 gccagtttaa aagtgacatg cagcattacg cccaactgtt ctggcattgc ccattaagtg
    11761 acgccgacta tcaaaaattg gtggcgagct aatgagaacc gtaaacggat gtattttgct
    11821 gctggcagct atcagtatca cttttgccgc cgtaagtggg gcttatcatc tcgatataca
    11881 gcaactgctg gcgctcattc tgcgtcaaga aaatgttcct gtgcaggaac aaattgtctt
    11941 ctggcaaatc cgcgtgccgc gtattcttgc tgcgctgttt ctgggcgcgg cgctggcagg
    12001 ggccggaacc acctatcagg gaatgttccg taatccctta gtgtcaccgg atattttagg
    12061 cgtctccgcc ggtgccggac ttggtgcctg cgcggcaatc ctgtggggat tatccattgt
    12121 ttacatccag ctgtacgcat tttgcggcgg gctgatggtg gtcgcgggcg tctggttgat
    12181 tacgcgtcgg gtgacccgcc acgatccgat cttaaccctg gtgctggtag gcattgcgtt
    12241 aggaacgttg tgcggagcgg ggatatcgct aataaaaacg ctggccgatc cttacaccca
    12301 gttaccgtca attaccttct ggttgctggg ggggctgtcc accgtgacct tacgcgatct
    12361 ctgctatgct gcaccgatca ttctcatagg ctccttgccc ctgttttttc tccgctggcg
    12421 catgaattta ctcacgcttt ccgacgacga agcccgttcg ctgggactga acgtgacacg
    12481 tctgcgcttt ggattaattg tctgcgccac actaatcacc gccagcacgg tagcaatagc
    12541 gggcataatt ggttgggtcg gcctggtcgt tccgcacatt gcccgcttgt tgaccggtca
    12601 taaccatcag caactgttgc ccatggcaat gtgtactggc gcgattctgc tcctgcttac
    12661 cgatacgctg gcacgcagca ttggaacaac agaaatccca cttggcattc tgaccgcttt
    12721 tgttggtgca ccgtttttcc tgtttttgtt gttacgcgga ggtcgccagt gactttgctt
    12781 gccgtccgtc atgcgtctct gggttactcc cgccatccgg tgctacgcga cgtgtccttc
    12841 accctgtcgc aaggaacaat ttgttgtctg ttaggagcca atggttgcgg caaaaccaca
    12901 ctcatgcgct ccattcttgg cgttataccg ttattaaaag gcgagatctt actggatagc
    12961 ctccctgtgc agacactcag ccatcgccag cgcgcgcagg ctattgcctg ggtgccgcag
    13021 gcgcacgacg gcatttttgc ttttagtgtg ctggatatgg tgttgatggg cctggccccc
    13081 accataggcg ctttttccgt tcccggtaag caagaacgcc tgaaagcaac ggagcaactg
    13141 gaaaagctgg gcattttgca tctggcgaca cgacgctgga atacgcttag cggcggtgaa
    13201 cgtcagctgg ctttaatcgc ccgtgcactc gtccagcaac cgcgcttgtt attactggat
    13261 gagcctgcct ccagcctcga tttcggtcat cagatccaac tactggacac gctggcgcag
    13321 ctcaaaaata acggcatgac aatgttaatg tcgactcatc atccgctcca tgccaacgcc
    13381 attgccgaca gcattatcca ggtagagcca gatggacgag taacacaagg cttaccgacc
    13441 gaacagttaa caacgaacaa actggctgcc ctgtaccggg tttctgccga ccagattcac
    13501 catcacctct ctgcaattaa gcactaagga aaaatcatgc tcattgacga tattgatttt
    13561 gcagaccttt accttcagca actcaaactg gcccaccgca cagaaaaaac gccggatcac
    13621 tgggatcaac gggcggaaaa aatggcggaa aattgcgcct cgcctacgga cagctacctg
    13681 caacaactga ccgctaaaat tgatctgcaa ggcgcgcaga ccttattcga tatgggctgc
    13741 ggaccgggta ccgtatcgct ggcgctggct gacaaactca cgacgattta tggcgttgat
    13801 tacagtcagg ggatgctgaa ggttgccgcc cgtcgcgccg ccgcgctaaa ggcagacaat
    13861 gtccactgga tccagcgcgc ctgggaagag gactggagtg acctgccacg ctgcgatatt
    13921 gccatggctt cccgctccac tctggtggct gatatgcgcc aggcaatgag caaactgaat
    13981 aatcaggcac ggcttcgcgt ctatacgact catctggtca gcacctcgtt cgtctctcca
    14041 gccattcagc gggctgctgg tcgggaagtg atcgaattac ccaactatat ctttgcgctc
    14101 aacgtcctgt atcagatggg aatttatgct cacgttgatt ttatccgtgg acaaaattgc
    14161 caacaggata acagcacctg ggaacgtttt gagcagaacg tcagctggag tctgggcgcg
    14221 cttaacgacg atgagcgtga gcggttatat cgctggtatc aacaacaaga tgctcgtgcc
    14281 ctcgcccctg ccagccgcga ctgggcgctc atctggtggg atagcgtgca gccggaggca
    14341 ctgcgatgat tttcctttct caggcacaaa tcgatgcgtt acttctggaa gatatccagg
    14401 gcggtgacct gaccacgcga gcattaaata ttggacacca gcatggctat atagagtttt
    14461 ttcaccgtca gggcggctgc gtcagcggaa tttctgtcgc gtgtaagatg ttaaccacgc
    14521 tggggttaac cattgatgac gcggtcagcg acggttcaca agcgaacgcg ggtcagcggc
    14581 taatccgtgc gcagggtaat gccgcagcac ttcatcaggg atggaaggca gtccagaatg
    14641 tgctggagtg gagttgcggt gtttctgatt atctcgctca aatgctggcg ttacttcgtg
    14701 aacgttaccc tgatggcaat atcgcctgca cccgaaaagc gattccgggc acccgcttac
    14761 tggcctcgca ggcaattctg gctgccggag gactgattca tcgtgccgga tgtgcggaaa
    14821 ccatattatt gtttgccaac caccgtcatt ttcttcatga caatcaggac tggtcaggcg
    14881 caatcaatca gttacgtcgc cacgcacccg agaagaaaat agttgttgaa gccgacacgc
    14941 cgaaagaggc aatcgccgcg ttacgcgcgc aaccagacgt acttcagctc gacaaattta
    15001 gtccacaaca ggcaactgaa attgcgcaaa tagcaccgtc gctggctccc cactgcacgc
    15061 tggctctgac cggcggaatt aatctgacaa cactcaaaaa ttacctcgac tgcggcattc
    15121 gcctttttat tacctccgcg ccttattacg cgacaccggc tgacattaaa gtcagtctgc
    15181 aacccgcagc ctctatttaa ttctttgtta aacggaattt attctatgag actcaaaaaa
    15241 cgttatctct gcacagcatt atcacttgcc tttacccagc aggccgtagc ggctcaggag
    15301 agtgacacgc tgaccgtatg gtccagtccg gtatcatcga cgacgaccac cgttctcgat
    15361 caacccacca tgaaggccct ggataaacag aatgtcgctc aggcattaag tgtcgtcccc
    15421 ggcgtggtgc tgcaaaagtc aggtagccgc aacgaagaac aggtcaaagt tcgtggcttt
    15481 gatagtcgtc aggtgccggt ctatttcgac ggtgtgccca tttatgttcc ctatgacggc
    15541 aacctcgatc tggcgcggat tctgaccaac aatttggggg cagttgaagt ttccaaaggg
    15601 tattcgtcgc tgcttcaggg gcctaatcag atgggcggag ccattaatat caccacccag
    15661 aagccaacaa aacctctgga agcaaatctg ggatatcgcc agggatggag ccgtagccag
    15721 gacaatgcct acgatatgca tgcttcattt gccgccagca gcgatctggg gtatttgcaa
    15781 gtcagcggta gccaggtaaa gcaggatttt ctcggcctgc cgcatggtgt aaataatgat
    15841 attgcaggca aacacggcaa gatgattaat tcatcggctg atgataaacg cggcattgtg
    15901 aagctcggtt ttacaccacg cgaaaacgat gaatacacat tgacttacat taagcaggat
    15961 ggtgaaaaag ataacccgcc atacagtgga aatagtggtc aaaaatcacg ctactggcag
    16021 tggccagagt atgacaaaga aagtttttat tatcagggaa cgacccaact aaacgatcgt
    16081 tttaccctga aaagtcggct gtatcgcgac acctttgaaa atacgctgat gatgtacaac
    16141 tcgctggctg atttgaaaaa taaaaaaggc agctacagcc attattccga ttacagcgac
    16201 ggtgccgggt tacaactggc agccgatgtg cgtgaaaacg atctgctgtc gtttgccgtt
    16261 aactggaaag atgacgtaca ccgggaaaaa ggtgcgccgc acgccgctta cgatcgctat
    16321 gaagatcgta cctggtcgct cgccagtgaa tatcaatggg ctgctgccga taatgtcgat
    16381 gtcgtggctg gaatcagcta tgactggcgc gatagcgtag aagcgaaaaa acatgagaaa
    16441 gatggaagca tcacccatta tgacgacaac aatcagtcag cttttaactg gcaggtgatg
    16501 gggaaatacc actttgccaa tgaagacacg ctggcgcttt cgtactatga ccgcacacgc
    16561 tttccgacgc tgaaagaacg ctataccacg tccaaacctg cgtataacca gatagcgatt
    16621 gttaacccgc agctcaaacc ggaacgcgcg cgcggggtgg atttaacctg gaatggtgcc
    16681 ttcacgcacg actgggggtt tgaggtcagc gtttactata accgggtgag tgatgccatc
    16741 ctctcgcaca atatcgatgc cgataccatt caaaatcaga acagcggcac ggtggattac
    16801 agcggtctgg atgccggtat taaggggaaa atcagcaata tactggatgt aggattgagc
    16861 tacgccctga tccacgctga tgccaaacgc aaagacatcg gcaagataac cgatctgcca
    16921 acgcagacaa tgaccgcatg gatgactctc aaaccgtggg agccgttaag cgtaacgctg
    16981 tcggaagagg cgcgttcctc cagctacagc aacagtgacg gttcacaaaa agccgccggt
    17041 tttgcggtga cccacattcg agccgattac accttaggtc atggcttcag cgtcaatgcg
    17101 tcggtcaata acctgtttga tacccaatat gcctacagtg aagggtttat tgaagaaggt
    17161 cgcaatttct gggcaggtat cgagtatacg ttctgatgtg aaagcggata gcggcatctg
    17221 tatcgctatc cgccttatga aaatcttatt ctcttcgcag gaggcggaat acgcccagca
    17281 ccagaatagc gcccactacc gccaccagga agctgtgcaa attaaaaccg ctgatggagc
    17341 cgccaatgcc aaacatggtc gccagccagc cgccgaccac cgcaccgact atcccgagaa
    17401 tacaggtcag gaaaaatcca ccaccatcac gccccggcat gattagcttg gcgataatgc
    17461 cggctatcag gccaaaaata atccaggcaa taattcccat gttcaggccc tcgtttcgat
    17521 gaatcggacg caagaaatgc ttacgtcaac gctctaagta taggcaaggg cggaatcgat
    17581 gctgggcgta tgtcggctga tttaagaaca acatgtaaaa aatattcacg catttgtatt
    17641 aagttttgtt aactgtgacc gataaaccaa agacagtttg tcagtcagga gtttttccgc
    17701 gtgagtcatt accatgagca gttcctgaaa caaaatccgt tagccgtcct gggcgtgtta
    17761 cgcgatttgc acaaagccgc aattcctttg cgtatcagtt ggaatggcgg gcagctgatc
    17821 agcaaaatac tggcaataac cccggataaa ctggtgctgg atttcggcag ccaggccgaa
    17881 gataacaacg ctgtgttaaa ggcgcagcac attaccatta ccgccgaaac ccagggcgcg
    17941 aaagtcgaat ttactgttga acaactacag cagagtgaat acttgcagct tccggcattt
    18001 attaccgtac cgcctcccac cttgtggttt gtacaacgac gccgatattt ccgcatctcc
    18061 gccccactcc atccgcctta tttttgccag accaaactgg cggataacag tacgttacgt
    18121 ttccgcctgt atgatttgtc gttaggcggc atgggcgcat tactggaaac agcaaagcct
    18181 gccggattgc acgaaggcat gcgcttcgct cagattgaag tcaacatggg gcaatggggt
    18241 gtttttcact ttgacgccca gttaatctca atcagcgagc gcaaagtgat tgatggcaag
    18301 aatgaaacca tcaccactcc ccgtctgagc ttccgttttc ttaacgtcag cccgacggtg
    18361 gagcggcaat tacagcggat tattttctct ctcgagcggg aagcccggga gaaagcggac
    18421 aaagtgcgcg actgatttac atcgcgtcca gtgcctgctc aagtttgtag atatagcgcg
    18481 gagcctgcgg cgcaggatga tttcgcgcta cgtgatcgag gaactcgtca gcatccagat
    18541 cgttgatttt gctgatcgcc tttttccgat ctgacgagaa agtccgtagc agcgcacctg
    18601 ccccgttagc gtatgacacc accagcgcat attgcagtac cttcggatct tcaatacctg
    18661 ccagcgggcc ggtttccaga atattcaggt aagccgcccc cattgaaata ttacgctccg
    18721 gatttttcag ctcgctggtc gtcggctcac cactccagcc catacggcga taaacatcac
    18781 gtccggaggt tgaagctttt aactgcatca aaccaatggc attcgattta ctcaccgcat
    18841 tcggattacc acccgattcg atagcgataa tcgctgtgat caattgtgga tcgacgcccc
    18901 aggctgcacc ggctttctgg cttattggca tccactgcat cgcacgttgc accggaactt
    18961 tcgcgttcca cggcgggttc gtgtagtcat gctttgatga acaacctgct aataacacaa
    19021 tcaaaaaggc aaaccatctc aatttcactt cttctatcct tatagcctgg aatcgccgtt
    19081 gaggtgacaa cggcacacct aagcggcatg atatacattt cgtttctgtt gtcagcaagg
    19141 aattgccatg tctctgtttc acttaattgc cccatcgggt tactgcatta aacagcacgc
    19201 cgcgctgcgt ggtatccagc gccttaccga cgcggggcat caggtcaata acgtcgatgt
    19261 tattgcccgt cgttgtgagc gttttgcggg tacggaaacg gagcgtctgg aagatctcaa
    19321 ttcccttgcc agactgacta cccccaacac tatcgtgctg gctgtacgcg gtggttacgg
    19381 tgccagtcgt ttactggcgg atattgactg gcaggcactg gtggctcgcc aacaacatga
    19441 cccgttgctc atttgcggac atagcgattt taccgccatt cagtgcggtc ttctggcgca
    19501 tggcaatgtc atcaccttta gcggccccat gctggtagca aactttggcg cggatgaact
    19561 gaacgccttt accgagcacc atttctggct ggcgttacgc aatgaaacct tcaccattga
    19621 atggcaaggg gaaggcccga catgtcagac cgaaggcacg ttatggggag gcaatctcgc
    19681 gatgctgatt tcactgattg gtacaccgtg gatgccgcaa atcgaaaacg gtattctggt
    19741 gctggaagat attaacgagc accctttccg cgtcgaacgt atgctgttgc agctctatca
    19801 tgctggaatt ttgccacgtc agaaggcgat tattctcggt agctttagcg gcagcacgcc
    19861 caatgattat gacgcaggtt acaacctgga gtcagtgtac gcgtttttgc gttcccgcct
    19921 gtcgattcca ctcattaccg gcctcgattt tggtcatgaa ccacgcacgg ttacgctgcc
    19981 gttgggcgca catgccattc ttactaacac ccgggaaggc actcagttga cgattagcgg
    20041 tcatcctgtt cttaaaatgt aaaaaaatca cgcttcaggg ctaagtaaaa cgctgtctct
    20101 gccgctaata tggtagggtt tatataagag acagcgtaat caggagtaac cgaccgttgg
    20161 atgccacaac aataattagc cttttcatct taggatccat ccttgtcacc agcagtatat
    20221 tacttagttc attttcttcc cgtcttggca ttcctattct ggttattttt ctggcgatcg
    20281 gcatgctggc gggagtcgat ggcgtcggag gtatcccgtt tgataattac cccttcgcct
    20341 acatggtaag taacctggcg ctggcgatta ttttgctcga cggcgggatg cgcactcagg
    20401 ccagttcctt tcgtgtggcg ttaggaccgg ccctgtcgct ggcgacgctg ggcgtgctta
    20461 tcacctctgg tttaaccggc atgatggcgg cgtggctgtt taatcttgat ttgattgaag
    20521 gcttattaat cggcgctatc gtcggctcca ccgatgctgc agcggtcttt tctctgctgg
    20581 gtggtaaggg gcttaacgaa cgtgttggct cgacgctgga aattgaatcc ggcagtaatg
    20641 atccaatggc ggtctttctg acgattaccc tgattgcgat gatccagcaa catgaaagca
    20701 gtgtcagttg gatgttcgta gtcgatattc tgcagcaatt tggcctcggg attgtcatcg
    20761 gtcttggcgg tggttattta ctgctgcaaa tgattaatcg catcgccctg cccgccggat
    20821 tatatccatt gctggcatta agcggcggta ttttaatttt tgctttaacc acggcgctgg
    20881 aaggtagcgg tattctggct gtttatctgt gcggttttct gctgggtaat cgcccgattc
    20941 gcaaccgcta tggcatcctg caaaatttcg acggcctcgc ctggctggcg caaatcgcca
    21001 tgttcctggt gctggggcta ttggttaacc caagcgatct gctgcctatt gctattccgg
    21061 cgctcatttt gtccgcatgg atgatattct tcgctcgtcc tctttcggta tttgccggat
    21121 tgctaccgtt ccgtggtttt aatctgcgtg agcgcgtgtt tatcagctgg gtgggattac
    21181 gcggcgcggt gccaatcatc ctggccgtgt tcccgatgat ggcggggctg gagaatgcgc
    21241 gactgttctt taatgtcgcc ttctttgtgg ttctggtttc actgctcttg cagggaacat
    21301 cactctcgtg ggcggcgaaa aaagccaaag tggtcgttcc gccagtggga cgtccggtgt
    21361 cacgcgttgg tctggatatt catccggaaa atccgtggga gcagtttgtt tatcagttga
    21421 gtgccgataa atggtgcgtg ggcgcggcac tgcgtgatct gcatatgcca aaagagacgc
    21481 gtattgcggc actgtttcgt gataaccagt tgcttcatcc taccggcagc acccgactgc
    21541 gcgaaggcga tgtgttgtgt gtaattggtc gggaacgcga tctcccggcg ctcggtaaac
    21601 tgttcagcca gtcgccgccg gtcgcgctgg atcaacgctt ctttggtgac ttcattctcg
    21661 aagccagcgc caaatatgca gatgtggcgc tgatatatgg cctggaagac gggcgagaat
    21721 atcgtgataa gcaacaaacg ctgggtgaaa ttgtccagca gttgttaggc gcggcaccgg
    21781 ttgtcggtga ccaggtagag tttgccggaa tgatctggac ggtggccgag aaagaagata
    21841 atgaagtgtt gaagattggc gtacgggtgg cggaagagga agctgaatct taaatttaat
    21901 acagcatcta tcgccggatg cgatgctttg cgaacgcagc ctgagcctgc atataaactt
    21961 gtcatttcct gattttgata tgctcatacc aacatcagaa gaaggttaaa ttatggatga
    22021 caaaccattg caggctctgg ctaatcaact caaaacgccg gctcaagaaa gtcagcataa
    22081 tggcttcttc tgactgcaac aggtgaagaa taaccagccc cgcattaccg gtatccccgt
    22141 caaccatcag gggccccagc ctgacatcgc ctgaacatat tcccccacgc aacctgccgc
    22201 actggcgtag cccacacaat acgcgtttca gactgctcag taactgtagt ggcgtttcat
    22261 ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc ttaataacga
    22321 ttatttccgg gtcgttcgct ctcgtatttt ctttaatacg tcagagctgc ctgggccgta
    22381 atacagcatg taccagaccc gacacggcat ttctgaccaa gggaaaaagt caacgatgag
    22441 tcgttatatc agcgcaaatg acgtcctgtg gcacctttgc ggtgaccaca ttcacgtctc
    22501 cggcgctgcc agaggatatt tatttcaagg atacgtagca ttatggccag cattaaagac
    22561 atccgcccat tcgcccccgg cattccccca tcttccgtta acccaggcgt acgcccacca
    22621 gggcagttct tcctgccggt ggaactcaag aatgaaattt tgcggaacat gaagtatatc
    22681 ggcccgcaaa tatttagctg gggtgatgac caccggcaga tcattgacct cagtaccgcg
    22741 ggcgaagtga aaatacatat ggtagacaat accatcatca cgttggagcg cctgaatccg
    22801 aatccgcaac ataacgagtt catgatggtc gccgaatggt gccaatgggc tatggagcgg
    22861 gatggtttgc aagaaaacct gcaaaaaaac ctgtacgaaa tcctggaaga aaaccagcaa
    22921 aacaaacagt cagaaattcc ccaagaagac ctgaaagaaa gtctggaaga aatcaaggaa
    22981 aatatcctgg aagaaaacct gccagcaagc cggattgaaa accgggcaga agcattgcgc
    23041 agaatgaagg aatgtctcat tacaagacag agtatgctca atcttagcaa cctgggattg
    23101 acttcactcc ctgagaattt gcctccacat ctgattgaat tttactgcag taaaaacgtg
    23161 ttgaccgcgt taccgaaggt gatgccaaag tggctgctgg tgcttgactg tacagataac
    23221 gtcttgatct tgttaccgaa ggtgcagccc tcaaaactga tggtactgaa gtgctatgaa
    23281 aactgtatta tctggctgcc tgagctgtcg actaacctga gagtgattaa ctgttctgaa
    23341 aacttcttgc aatttttacc gccgtcgatg ccccagtacc tgtataaact atcctgtgcc
    23401 ggcaacaata ttcactcaat acctgatgag atgctggaga acttgactcg cctgaaagta
    23461 tttgactgtt ctagtaacga tttgatctct gcaccacggc tgccgcccaa actaataatt
    23521 tactactgtg gagaaaacaa gtttaaaacg gtacaggtgc cacagcccca gagcctgaag
    23581 gtgtttgact gtaatggtaa cccgtgggac aaagacaatt taccgacgct gttcaaagcc
    23641 gtcgagggcc tgaaaaaaca gcagggcctg aaggaccttt tgggcttttt gcacaaggag
    23701 ggccagatga agctggagaa gctggagaag ctggaggatc tggatgacct tatggatcta
    23761 gagctcctgg atgacccggt actcctggag cgcgtgaagg tacaggagga cctggagctc
    23821 ctgaatcagc agttgggcct gttgattctg gaaagacaac aggactcgca gcctgttaat
    23881 caacattctg aacatgaacc cgaatctgca tcaagggtga agcgtgattt atctgaagtc
    23941 gagttcgagt caacaatgaa acgtaagcgt tttatgtaga acgcctgtgc ggtgaactca
    24001 ggttccgggg aaatagtgtc catcattgta atggacacta tcatgggaaa tggctctgtc
    24061 acatcaacaa ctacagttgc tgaccagccg gatggatgat tttttcgcaa ccagaagacg
    24121 ctgcctccga tccggcttac aacaagttac actgtcataa ccgggacacg taacgccagc
    24181 gcgcacatca actcatagcc caccgttccg gcagcggcgg cgacatcatc aattttgatc
    24241 tccttgcccc acagctcaac cggcgtacca atccccgcct gcgggcaagg cgttaaatca
    24301 accgccagca tatccatcga gacggtcccc accgtcatgg tgcgcacgcc gtccactaaa
    24361 acaggagtac cggtaggcgc atggcgcgga taaccgtcgg cgtatcctgc ggcgataatg
    24421 ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt agcccacacg ctcgcccgct
    24481 tttagcgtct ggacaccaat aatctcactg cttagcgtca tcaccggacg taatccggta
    24541 ttggcgatat cacgccactg accggacggc gaagcgccat acaaaataat gccaggccga
    24601 acccagtcaa aatgcgcttc cgggtgccac agagtcgccg ccgaattgga caacgaacgc
    24661 cgacactcca gcccttccgc cgcctgctca atacgctcca tcgcgccgga aattccatca
    24721 ggatgttccg cctcggcaaa atgcgacatc agggtcattt cgccaacatt cgccatcgcc
    24781 cgcaactgct gccagacggt aagtacgcga tcgggctgga agcccaaccg attcatccca
    24841 ctattcactt taagataaat atccaacggt gcttttagcc gcgcattttg cagtgctttg
    24901 agctgccagt tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat ctccagatcc
    24961 tgagcatgga aaaatccttc cagcatcagg atcggtcctt tccagccgcg ctcacgtaac
    25021 gttattgcct cttccaggtt aagtaatgca aagccatcgg tggccccgag cgcgctccag
    25081 atacgctcaa taccgtgccc gtaagcgttc gcttttacca ctgaccagac gcgcgcgtgc
    25141 ggcgcggcct ggcggacaat ggacagattc tgttttaatg cctgcagatc gaggctggcc
    25201 tgtatcggac gggtcatctc gtttccttag ctgtgcgcgc catgtaaatg gcccgggcgt
    25261 gatggcgtaa atccacggct gtagcgcgct acgcttagat cctcatacgg aatcgctggc
    25321 gtgcgaccag agagcagatc gcttaacaac tgaccggaac cgcaagccat cgtccagccg
    25381 agcgtgccgt gaccggtatt cagccacaga tttttaaagc gtgtacgccc gacaaccggc
    25441 gtgccgtctg gcgtcatcgg gcgcagaccc gtccagaaag tcgcctgctc gacatgaccg
    25501 ccgcgtggat agagatcgcg aaccaccatt tccagcgttt cacgacgcgg ttgcaacagc
    25561 tcggtgttaa aaccaacaat ttccgccatt ccgccaacac gaatgcggtt atcgaaacgg
    25621 gtaatggcga ttttgtaggt ttcatcaagg atagtagaca ccggcgcacc atcttcctgc
    25681 gcaatcggaa tagtcagcga gtagcctttc agcggataca ccggaatatc aacaatgcct
    25741 ttgagcatcg ccgtcgagta agaaccaaat gccatcacat atgcatcggc cttaatcact
    25801 tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg gtcaacgggc
    25861 gtattaaagc ggaatttaac ccccgcctgc tccgccatcc gcgccagatt ctgggtaaac
    25921 agctggcagt ctccggtttc atcattgggc agctggagcc cccccgtcag tttgtgcgct
    25981 acttctgcca gcgcgggctc cacttccgcc aggcggctgg attccaacag ttgatacggt
    26041 acgccagcat cttccagcac ggcgatatcg cgggtcgcat tttcatactg ttgttcggta
    26101 cggaacagtt gcagcgtccc accctgacgc ccttcatact gaatattggt ttcggcgcgc
    26161 aatgctttca ggcaatcacg gctgtattcc gccagacgca ccatccgccc tttgttttcc
    26221 atgtagtggc tggtgtcgca gttacgtaac atttgccaca tccatttcaa ctggaactgc
    26281 gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga acatccattt aatcgctttt
    26341 aaaggcacac ctggtgccgc ccacggtgct gcatatccgg gggagatttg cccggcattc
    26401 gcggcactgg tttccagggc ggcccccggc tctcgatcaa tgacggtgac ctcatgtcct
    26461 gcctgattta agtaccaggc gctggcaacg cctaccacac cacttcccag tatgacaact
    26521 cgcatagcca ctccgataac ggtagataca gtaaaagaat aataatctaa ttacatcttg
    26581 ataactcagt tgaaaatatt attcaacata cggctttttt atggtgagct ggctcacatc
    26641 tccctgactc tgcggcaata tgacgtcttt agtatctcct gctgaacgcc ctttttatcc
    26701 agcataaaat tccgttcaga agcggattag tggcactctg ataataagaa atcgcaaaga
    26761 aaaaaattct ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat gaggtgctcc
    26821 acacagagag cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt ctatgaataa
    26881 ggacaccaca cgtttgagcg atggacccga ctggacgttc gacctgctgg atgtttatct
    26941 ggcagagata gaccgggtgg cgaaactcta ccggctggat acctacccgc accagattga
    27001 agtgataacc tcagaacaga tgatggatgc ctactccagc gtcggcatgc caattaacta
    27061 tccgcactgg tcattcggta aaaagtttat cgagactgaa cggctgtata agcacggtca
    27121 gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt acctgatgga
    27181 agaaaacacc attaccatgc aagcgctggt gatggcacac gcctgctatg ggcataactc
    27241 tttcttcaaa aacaattact tattccgtag ctggaccgac gccagttcga ttgtcgatta
    27301 tctgattttt gcccgtaaat atattaccga gtgcgaagaa cgttatggcg ttgatgaagt
    27361 agaacggctt ctggactcgt gccacgcgct gatgaactac ggcgtggacc ggtacaaacg
    27421 cccgcaaaaa atctcgctgc aagaagagaa agcccggcag aaaagtcgcg aagagtatct
    27481 gcaaagtcag gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag agaaaacggt
    27541 tgctgaagcg cgccgctatc cgtccgagcc acaagaaaac ctgctctatt ttatggagaa
    27601 aaatgcgcca ctgctggaat catggcagcg tgaaattctg cgtattgtgc gtaaggtgag
    27661 ccagtatttt tatccgcaaa aacagactca ggtgatgaac gaaggctggg cgaccttctg
    27721 gcactacacc atccttaacc atctgtatga tgaagggaaa gtaacggaac gttttatgct
    27781 ggagtttttg cacagccaca ccaatgtggt cttccagcca ccctataaca gcccgtggta
    27841 cagcggcatc aacccgtatg ccctcgggtt cgccatgttc caggatatta aacggatttg
    27901 tcagtcgcca acggaagaag acaaatactg gttcccggat atcgccggtt ccgactggct
    27961 ggaaacgctg catttcgcga tgcgtgattt caaagatgag agttttatca gccagttcct
    28021 gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg ctggatgacg atcggcataa
    28081 ttatctggag atttccgcta ttcataatga agaaggttat cgggagatcc gtaaccggtt
    28141 atcgtctcaa tataatttaa gtaatctgga gccgaatatt cagatctgga acgtggattt
    28201 gcgcggcgac cgttcgctga cgctgcgtta cattccacat aatcgcgcac cgctagatcg
    28261 ggggcgcaaa gaagtactga agcatgtgca tcgcctgtgg ggatttgatg taatgctgga
    28321 acagcaaaac gaagacggca gcgtcgagtt gctggaacgt tgcccgccga gaatgggaaa
    28381 tctgtaaaaa caacaaaacc cctcgtttga ggggtttgct ttttaaacgg aagggattat
    28441 cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca aatctcgccg
    28501 ctctcatgcc catagcgacg cactgtttcg tatacctgat cgtgtgcgcc ttcactgcac
    28561 aatgccgaca gtttgtggta gaaacccagc gccagactgc gcgcttccgg attggcgaaa
    28621 tagtgacgac caatgcgcgt atacagccct ttcatcccgt taaggatcag accgtaaatc
    28681 gggttgccgg aagcaaacgc cagaccgcgg aatatgttgt aatccagctc ggcaaaggca
    28741 tcggcgtgat cggccacttc attagcggtt gccagtactt cctgcgcttt atcaggatgc
    28801 tgacgaaacg cggtgcgaat aaaaatcgtt gaaatattgg tacgcaccga cagcaaatta
    28861 tcaataagct gcggcacact ttcgtgatcc agtcgtgcca gtgtttcaag gatatttaaa
    28921 ccggaggttt cccagaaatt attcaccttc gtcggcttgc cgtgttgaat ggtcaaccag
    28981 ccatctcgtg ccagacgttg taacacttca cgtaacgtag tacgcgttac gccaattaat
    29041 tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt attccagata
    29101 ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat gaccatagtg
    29161 agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc taactacgct
    29221 gatagcagac aaaaagaaga aaaaagggat caaggcttca ttttccgcat ctctttaccc
    29281 ttgactatca agacttgcct cactcctgcc tgaccgttaa gctttgcgtt taaacataaa
    29341 aacatcattg attatttgta aggtaaggga aaccatcatg gagatctcct ggggccgcgc
    29401 gttatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc tcatcatttt
    29461 cttaatcgta aatccgttaa ttttcctcat cagccctttc gtcgctggct ggttgctggt
    29521 agcggaattt attttcactc tggcgatggc cctgaaatgc tacccgctac tccccggtgg
    29581 cctgttggct atcgaagcgg tattcatcgg catgaccagc gcggaacacg tccgtgaaga
    29641 ggtggcggca aatcttgaag tcttgctgtt actgatgttt atggtggcgg gtatctattt
    29701 tatgaaacag ctgttgctgt tcatatttac ccgtttgctg ttaagcattc gctccaaaat
    29761 gctgctgtcg ctctcttttt gcgtggcggc ggcgttcctc tccgcgttcc tcgatgcctt
    29821 aaccgtcgtg gcggtggtga tcagcgttgc agtcggtttt tatgggattt atcaccgcgt
    29881 agcctcttcc cgtaccgaag ataccgacct gcaagacgat agtcatatcg acaagcatta
    29941 caaagtggtt ctggaacagt tccgtggctt tctgcgtagc ctgatgatgc atgccggtgt
    30001 cggcaccgca ttaggcggcg taatgaccat ggtgggcgaa ccacagaacc tgatcatcgc
    30061 taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccag tgaccgttcc
    30121 ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt ggtttggcta
    30181 tggtgaaacg ctgccggaga aagtccgcga agtactgcaa cagtttgacg atcaaagccg
    30241 ccaccagcgt acccgtcagg ataaaatccg tctgattgtc caggcgatta ttggcgtctg
    30301 gctggtgact gcgctggcgt tgcatctggc ggaagttggc ttgattggtt tgtcagtcat
    30361 tattctggcg acatcattga ccggtgtcac cgatgaacat gctatcggta aagccttcac
    30421 cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcggtagtcg cggtgattat
    30481 cgaccaacaa ctgttttcgc cgattattca gtttgtgttg caggcatcgg aacatgctca
    30541 gctgtcgctg ttctatattt tcaacggtct gctgtcatcc atttcggata acgtcttcgt
    30601 ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta tcacgttgaa
    30661 gcaatacgag ctgctggcgg tcgccattaa taccggtacc aatctgccct ctgtcgctac
    30721 gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc cattgattcg
    30781 cctctcttat ggccgcatgg tgtggatggc cctgccttac accctcgtcc tgacactcgt
    30841 cggactactt tgcgtcgagt ttacgcttgc ccctgtaacc gaatggttta tgcaaatggg
    30901 ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt ttgccttttc
    30961 gcccgataat tgtccaattg cgtcctattt ttgctgcctc ctggtggcgc agcgaatgaa
    31021 ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg cgatttttga
    31081 accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttaccgct ctggcactgg
    31141 aactgacggc actgtggttc cagcatgtga tgttactgaa accttgcgtg ctctgtattt
    31201 atgaacgctg cgcattattc ggcgttctgg gtgctgcgct gattggcgcg atcgccccga
    31261 aaactccgct gcgttatgta gcgatggtta tctggttgta tagtgcgttc cgcggtgtgc
    31321 agttaactta cgagcacacc atgcttcagc tctatccttc gccgtttgcg acctgtgatt
    31381 ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa gtgtttgtcg
    31441 cctctggcga ttgcgccgag cgtcagtggg attttttagg tctggaaatg ccgcagtggt
    31501 tgctcggtat ttttatcgct tacctgcttg tcgcggtgct ggtggtgatt tcccagccgt
    31561 ttaaagcgaa aaaacgtgac ctgttcggtc gctaatccat tcagcgctcc tgcgggagcg
    31621 cttttttcct gccgctatgt ttatttgacc cgcagtaaat cagaacttcg cgttgtataa
    31681 cgtggtgaaa gcatagctcg cttcatctgc cattgttgtt ggatcccctg cccggcaaaa
    31741 tagagtgttc ctctgccctc tttagcattc agagtatcca ttaccgccat caattgctca
    31801 ctcccggggg gcggtgcgtt gtcatcgaat aaattgagct gcgcgactcc ctgactgaag
    31861 aaatctccca gcatcacgcc cgctttttgg tagcgatggc ccgcttgcca gatggcatcc
    31921 agagatcgcg tagcggcgtt aatgatatcc ctgctgtcct gagtgggcgt cagcagtttt
    31981 accgacgcgc tattgccgta ataaggttca ttgagcgcaa atggtgacgt cttaataaac
    32041 gtggaaataa accgacaata ttgatgctca ctgcgaagtt tttccgccgc ccgggcagcg
    32101 taactacaaa tggcctgccg catcgacgta taatccgtga tgcgttcacc aaacgaacgg
    32161 gaacagataa tttcctgctt cgtcggtgca aactcttcca gttgcaaaca gggttcaccg
    32221 tgcagttcac gcaccgttct ttcgagcacg acattaaaat gtttacggat aaaccggata
    32281 tctgtacccg ccaaatcgag aacggttttg atccccatcg cgtccagttt tttgctgatc
    32341 cgccgtccaa tcccccagac ttcatccacg gggagagcag acattaattt acgctggcgt
    32401 tccagatttg ataaatccac caccccaccc gtctgccgct gccatttttt tgccgcatga
    32461 ttggcaagct tagccagcgt tttggtctgg gcgatcccca cgccaacagt aagatgggta
    32521 cgttgtagca ccgttgcgcg aatttctcgg ccaaaatcag tcagatcgcg acaattacgc
    32581 acaccggtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg gggcgataat
    32641 tcttccagcg tcgacatcac ccgattgctc atatcggcgt aaagctcata gttactgcta
    32701 aagcaaacca cgccacagcg gcgaaacaga tctttttgtt tgaaccaggg atcgcccatt
    32761 ttaacgccca acgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc attattcgat
    32821 agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca gctggcataa
    32881 aacgcgttta catcacagag ggcaaacatc agcgcatcgc cttaacgacg tggatcacca
    32941 caccaaagac atccagcgta tcttcgctac tgatggtaat gggtgaatat gcactgttca
    33001 tgggaataag ctgcactgtc gggcgtagtt gcaatttttt caccgtaaac tcgccgtcaa
    33061 cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca atcagtaaat
    33121 caccgtcact aatcccaccg tcaatcatag aatcaccact tgctttgacg aagtaagtcg
    33181 cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcaacg taatctgcgg
    33241 ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc acaatttcgc
    33301 ggagatccgc aggcttgata aacaacataa taatctgcct gaagttatac tgtttttata
    33361 tacagtagtc tgttcttgcc agcagatcaa tactgattca ggctatcaat atttgtcgct
    33421 gcatagactg ctgatttttc gttctcttat cctgtgctca cgcggccttc tggcgacgac
    33481 gctcatccag cagaaatgaa aaatatcacc cggctaaaaa tagaatagaa gcatt
//
LOCUS       DARNVM010000034        33378 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.034, whole genome shotgun sequence.
ACCESSION   DARNVM010000034 DARNVM010000000
VERSION     DARNVM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.034"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(56..964)
                     /gene="melR"
                     /locus_tag="SI621_001500"
     CDS             complement(56..964)
                     /gene="melR"
                     /locus_tag="SI621_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HEI4064232.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1247..2602
                     /gene="melA"
                     /locus_tag="SI621_001501"
     CDS             1247..2602
                     /gene="melA"
                     /locus_tag="SI621_001501"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEI4064233.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2705..4126
                     /gene="melB"
                     /locus_tag="SI621_001502"
     CDS             2705..4126
                     /gene="melB"
                     /locus_tag="SI621_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HEI4064234.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4265..4894)
                     /locus_tag="SI621_001503"
     CDS             complement(4265..4894)
                     /locus_tag="SI621_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEI4064235.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5017..6663)
                     /gene="fumB"
                     /locus_tag="SI621_001504"
     CDS             complement(5017..6663)
                     /gene="fumB"
                     /locus_tag="SI621_001504"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HEI4064236.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILQDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6741..8081)
                     /gene="dcuB"
                     /locus_tag="SI621_001505"
     CDS             complement(6741..8081)
                     /gene="dcuB"
                     /locus_tag="SI621_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HEI4064237.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8652..9371)
                     /gene="dcuR"
                     /locus_tag="SI621_001506"
     CDS             complement(8652..9371)
                     /gene="dcuR"
                     /locus_tag="SI621_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HEI4064238.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9368..10999)
                     /gene="dcuS"
                     /locus_tag="SI621_001507"
     CDS             complement(9368..10999)
                     /gene="dcuS"
                     /locus_tag="SI621_001507"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HEI4064239.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11179..11409
                     /locus_tag="SI621_001508"
     CDS             11179..11409
                     /locus_tag="SI621_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEI4064240.1"
                     /translation="MDQDLLDGGYRCYTGEKIDVWFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            11421..11693
                     /locus_tag="SI621_001509"
     CDS             11421..11693
                     /locus_tag="SI621_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4064241.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11922..12218
                     /gene="ghoS"
                     /locus_tag="SI621_001510"
     CDS             11922..12218
                     /gene="ghoS"
                     /locus_tag="SI621_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HEI4064242.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12246..12419
                     /gene="ghoT"
                     /locus_tag="SI621_001511"
     CDS             12246..12419
                     /gene="ghoT"
                     /locus_tag="SI621_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HEI4064243.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="SI621_001512"
     CDS             complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="SI621_001512"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEI4064244.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERIFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="SI621_001513"
     CDS             complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="SI621_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HEI4064245.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFAARFLFSTPTNMIQESND"
     gene            complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="SI621_001514"
     CDS             complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="SI621_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HEI4064246.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSNWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="SI621_001515"
     CDS             complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="SI621_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HEI4064247.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="SI621_001516"
     CDS             complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="SI621_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HEI4064248.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(21890..21965)
                     /locus_tag="SI621_001517"
     tRNA            complement(21890..21965)
                     /locus_tag="SI621_001517"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21930..21932),aa:Phe,seq:gaa)
     gene            complement(22072..22647)
                     /locus_tag="SI621_001518"
     CDS             complement(22072..22647)
                     /locus_tag="SI621_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEI4064249.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(22684..24381)
                     /gene="dsbD"
                     /locus_tag="SI621_001519"
     CDS             complement(22684..24381)
                     /gene="dsbD"
                     /locus_tag="SI621_001519"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEI4064250.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVLADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQVRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMLLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(24357..24695)
                     /gene="cutA"
                     /locus_tag="SI621_001520"
     CDS             complement(24357..24695)
                     /gene="cutA"
                     /locus_tag="SI621_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEI4064251.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(24810..26111)
                     /gene="dcuA"
                     /locus_tag="SI621_001521"
     CDS             complement(24810..26111)
                     /gene="dcuA"
                     /locus_tag="SI621_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HEI4064252.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(26229..27665)
                     /gene="aspA"
                     /locus_tag="SI621_001522"
     CDS             complement(26229..27665)
                     /gene="aspA"
                     /locus_tag="SI621_001522"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEI4064253.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            28002..28478
                     /gene="fxsA"
                     /locus_tag="SI621_001523"
     CDS             28002..28478
                     /gene="fxsA"
                     /locus_tag="SI621_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEI4064254.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(28494..29750)
                     /gene="yjeH"
                     /locus_tag="SI621_001524"
     CDS             complement(28494..29750)
                     /gene="yjeH"
                     /locus_tag="SI621_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HEI4064255.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALGINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            30026..30319
                     /locus_tag="SI621_001525"
     CDS             30026..30319
                     /locus_tag="SI621_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEI4064256.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            30363..32009
                     /gene="groL"
                     /locus_tag="SI621_001526"
     CDS             30363..32009
                     /gene="groL"
                     /locus_tag="SI621_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEI4064257.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            32147..32500
                     /locus_tag="SI621_001527"
     CDS             32147..32500
                     /locus_tag="SI621_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEI4064258.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(32550..>33378)
                     /locus_tag="SI621_001528"
     CDS             complement(32550..>33378)
                     /locus_tag="SI621_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HEI4064259.1"
                     /translation="KSNNFIALALKIKEPRNKESLFFLSVMELRDLLIALESRLHQKH
                     KLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFV
                     LTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDN
                     GNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEVEAITR
                     RMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
BASE COUNT         8621 a         7998 c         8325 g         8434 t
ORIGIN      
        1 tcactgctta ttatcgacgg ctaaactatt tttttggctg atactgatat cgtctttagc
       61 cgggaaacgt ctggcggcgc tgttggctaa gtttgcggta ttgttgcggt gacatgccga
      121 catatttgcc gaacgtgctg taaaaacgac tacttgaacg aaagcctgcc gtcagggcaa
      181 tatcgagaat acttttatcg gtatcgctca gtaacgcgcg aacgtggttg atgcgcatcg
      241 cggttatgta ctgtttcatc gtcaattgca tgacccgctg gaatatcccc attgcatagt
      301 tggcgttaag tttgacgtgc tcagccacat cgttgatggt cagcgcctga tcatagtttt
      361 cggcaataaa gcccagcatc tggctaacat aaaattgcgc atggcgcgag acgctgtttt
      421 tgtgtgtgcg cgaggtttta ttgaccagaa tcggttccca gccagagagg ctaaatcgct
      481 tgagcatcag gccaatttca tcaatggcga gctggcgaat ttgctcgttc ggactgttca
      541 attcctgctg ccagcggcgc acttcaaacg ggctaagttg ctgtgtggcc agtgatttga
      601 tcaccatgcc gtgagtgacg tggttaatca ggtctttatc cagcggccag gagagaaaca
      661 gatgcatcgg cagattaaaa atcgccatgc tctgacaggt tccggtatct gttagttggt
      721 gcggtgtaca ggcccagaac agcgtgatat gaccctgatt gatattcact ttttcattgt
      781 tgatcaggta ttccacatcg ccatcgaaag gcacattcac ttcgacctga ccatgccagt
      841 ggctggtggg catgatatgc ggtgcgcgaa actcaatctc catccgctgg tattccgaat
      901 acagcgacag cgggctgcgg gtctgttttt cgtcgctgct gcacataaac gtatctgtat
      961 tcatggatgg ctctctttcc tggaatatca gaattatggc aggagtgagg gaggatgact
     1021 gcgagtggga gcacggtttt caccctcttc ccacagcggc gaggggagcg tccgagcatc
     1081 atgaggccga aaactctgct tttcaggtaa tttattccca taaactcaga tttattgatg
     1141 cttcacgtag gatctgagtt tatgggaatg ctcaacctgg aagccggagg ttttctgcag
     1201 attcgcctgc catgatgaag ttattcaagc aagccaggag atctgcatga tgtctgcacc
     1261 caaaattaca tttatcggcg ctggttcgac gattttcgtt aaaaatattc ttggtgatgt
     1321 gttccatcgc gaggcgctga aaacggcgca tattgccctg atggacattg atcccactcg
     1381 cctggaagag tcgcatattg tggtgcgcaa gctgatggat tcagcagggg ccagcggcaa
     1441 aatcacctgc cacacccaac agaaagaagc cttacaggat gccgattttg tcgtggtggc
     1501 atttcagatt ggcggttatg aaccttgcac ggtgactgat ttcgaggtct gtaagcggca
     1561 tggtctggaa caaaccattg ccgatacgtt ggggccgggc ggtattatgc gcgcgctacg
     1621 taccattccg catctgtggc aaatttgcga ggacatgacg gaagtctgcc ccgatgccac
     1681 catgctcaac tacgttaacc caatggcgat gaatacctgg gcgatgtatg cccgctatcc
     1741 gcatatcaaa caggtagggc tgtgccattc ggtgcaggga acggcggaag agctggcgcg
     1801 tgacctcaat atcgacccgg ctacgctgcg ttaccgttgc gcaggtatca accatatggc
     1861 gttttacctg gaactggagc gcaaaaccgc cgacggcagt tacgtgaatc tctacccgga
     1921 actgctggcg gcttatgaag cagggcaggc accgaagccg aatattcatg gcaatactcg
     1981 ctgccagaat attgtgcgct atgaaatgtt caaaaagctg ggctacttcg tcacggaatc
     2041 gtcagaacat tttgctgagt acacaccgtg gtttattaag ccaggtcgtg aggatttgat
     2101 tgagcgttat aaagtaccgc tggatgagta cccgaaacgc tgcgtcgagc agctggcgaa
     2161 ctggcataaa gagctggagg agtataaaaa cgcctcccgg attgatatta aaccgtcacg
     2221 ggaatatgcc agcacaatca tgaacgctat ctggactggc gagccgagtg tgatttacgg
     2281 caacgtccgt aacgatggtt tgattgataa cctgccacaa ggatgttgcg tggaagtagc
     2341 ctgtctggtt gatgctaatg gcattcagcc gaccaaagtc ggtacgctac cttcgcatct
     2401 ggccgctctg atgcaaacca acatcaacgt acagacgctg ctgaccgaag ccattcttac
     2461 ggaaaatcgc gaccgtgttt accacgccgc gatgatggac ccgcatactg ccgccgtgct
     2521 gggcattgac gaaatatatg ctcttgttga cgacttgatt gctgcccacg gcgactggct
     2581 gcctggctgg ttgcaccgtt aaaacgcgac taaacgctac tgcgccgggg gatttattcc
     2641 ggcgcacact tctgacgata ccaataacag aaggcgggcg ttggtaacaa cgacccgata
     2701 ccctatgagc atttcaatga ctacaaaact cagttatgga tttggagcgt tcgggaagga
     2761 ttttgcgatc ggcattgtgt atatgtacct catgtattac tacaccgatg tcgtcgggct
     2821 gtctgtgggt ttggtcggta ctttgtttct ggtggcgagg atctgggatg ctattaacga
     2881 tccgattatg ggatggattg taaatgctac gcgatcgcga tggggtaagt tcaaaccctg
     2941 gatcctgatc ggtacgttgg caaactctgt aatcttattt ctcctcttta gtgcgcatct
     3001 gtttgaaggt actactcaga ttgtctttgt ttgcgtgacc tacatcctct ggggcatgac
     3061 ttacaccatt atggatattc ccttctggtc gctggttcca accataacgc tcgataaacg
     3121 tgagcgcgaa caactggttc cttatccgcg tttttttgcc agtctggcgg gctttgttac
     3181 ggcaggtgtg acgctaccat ttgttaatta tgtcggcggt ggcgatcggg gatttggctt
     3241 tcagatgttc actctggtac tgatcgcctt ttttattgtt tcaaccatca tcactctgcg
     3301 caatgtgcat gaagtctttt cgtcagacaa tcaaccgtct gctgaaggaa gccatctgac
     3361 acttaaagcc atcgttgcgc ttatttataa aaacgatcag ctttcatgcc tcttgggtat
     3421 ggctcttgct tataatgtag ccagcaatat tattaccggc tttgctatct attatttctc
     3481 atatgttatc ggtgatgcgg atttgttccc ctattatctg tcgtatgcgg gagctgctaa
     3541 tctggtgacg ttagtattct tcccacgctt agttaaatca ttatcccgac gcattttatg
     3601 ggccggagca tctattcttc cggtgttaag ctgtggtgtt ctcctgttaa tggcattaat
     3661 gagctatcac aacgtcgtcc tcattgtgat tgcgggtatc ttgctgaatg tgggaacggc
     3721 gcttttctgg gtattacagg tcatcatggt ggcagatacc gttgattacg gtgaatataa
     3781 actgcacgta cgctgtgaaa gcatcgctta ctccgtgcag actatggtgg tgaagggcgg
     3841 ttcagccttt gcggcttttt tcattgcggt tgtgttaggg atgattggct atgtaccgaa
     3901 tgttgaacag tctacgcaag ccctattagg tatgcagttt attatgattg ctctaccgac
     3961 tctgtttttc atggtaacgc tgattctcta cttccgtttc tatcgcctca atggtgacac
     4021 gctgcgcagg atccagatcc atctgctgga taaatatcgc aaagtaccgc ccgagcctgt
     4081 tcatgctgat attccggtcg gtgcagtgag tgatgtgaaa gcctgacgtg gacaatagct
     4141 aacgaaaatg gcctgatgcg atatgtttat caggccatgt cttaatgtaa tgctttggat
     4201 ttgcaaattt tgtagaccgg ataaggaatt cacgccgcat ccggcgtcaa caaagcgcac
     4261 gttgttatcc ggttatcaag ccaaagcgcc gtagctggcg gcaatgaaag cgagcgagaa
     4321 atatcaccgt cgttaatgcg ccaagcagcg cgcagaacat atcggattgc gtgtcccact
     4381 ggtcgccctg ggtgccgaga aaatcatccg ctccctggcc cattgccagc gccgcccacc
     4441 actcgattaa ttcatacatc gcgcttatcg ccagcgccac gcagcagacc agaaacgcca
     4501 ccatcttatg tccgcgaacg tacataccgc gcacaaggat ctctcgtgcc accagtgcag
     4561 gcaccagccc ctggaaaaaa tgccccagtt tgtcatacgg attacggctc aaccccagcc
     4621 attcctgcac ctcaaaacca acggggactt tcgcgtaggt gtattgtccg ccgaccatca
     4681 ggatgatggc gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga taacgtctgg
     4741 cggtggcaag cagtagcggc acgacaataa tcaccggtgt cacttccatc agccaggtga
     4801 gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg
     4861 tgttaagaat taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata
     4921 ctgactattc accagcaagc gtgaaattac aatcgcaaaa gaggtggccc agggggatca
     4981 cctggcagca tgctgccagg cgctgggccg aagaggttac ttagtgcagt tcgcgcactg
     5041 tttgttgacg atttgctgga agaagtcgtt acctttgtca tcgaccagga taaacgccgg
     5101 gaaatcttct acttcgattt tccagatagc ttccataccc agctccggat aagcgacgca
     5161 ttccagatgc ttgatgctct gctgcgccag taccgccgcc ggaccgccga tgctaccgag
     5221 gtagaagccg ccgtgtttat gacacgcgtc ggtaacctgc tggctgcggt tgcctttcgc
     5281 cagcatgatc atgctgccgc cgtgggattg cagcagatcc acgtaggagt ccatacgacc
     5341 tgcggtggtt gggccaagtg aacctgatgg ataaccggca ggggttttcg ccggacccgc
     5401 gtagtagatc gggtgatctt tgatgtactg cggcagttct ttaccggcgt caatcagctc
     5461 tttcagcttg gcgtgtgcaa tatctcgacc cacaataatg gtgccggtga gcgacaagcg
     5521 ggtggatacc gggtattgcg aaagctgggc gaggatctct ttcatcgggc ggttaaggtc
     5581 aactttcacc gcttcgcctt caccggcctg gcgcagttct tccggaatgt actggcctgg
     5641 gttgtgttcc agtttttcga tccagatacc ttcgcggttg attttcgctt taatgttacg
     5701 gtcagcggag caggagacgc ccatgcctac cgggcaggat gcaccgtgac gtggcagacg
     5761 gataacgcga atgtcgtgcg cgaagtattt accaccaaac tgcgcgccaa gaccgagttt
     5821 ctgggcctct tccagcagtt cctgttccag ctggacatcg cggaacgcct gaccatgttc
     5881 gttcccttcc gtcggcagtt catcgtaata gtgagcgctt gctaacttga cggttttcag
     5941 gttggtttcc gcagacgtac cgccaatcac aaacgcgata tggtacggcg ggcaggctgc
     6001 agtaccgagg gtacgcattt tctcaacgag gaagtttttc agtttgccgg gagtcagcag
     6061 ggctttggtt tcctggtaga gataggtttt gttggcagag ccgccgcctt tagcgacaca
     6121 gaggaatttg tactcatcgc catctaccgc gtacaggtcg atttgcgcag gcaggttagt
     6181 gccggtgttg acctctttgt acatgtccag cgccgcattc tgtgaatagc gcaggttgtc
     6241 ttcgatatag gtgttataga cgcctttcga cagcgcttct tcatcaccgc caccggtcca
     6301 cacgcgctgg ccttttttac cgacgatgat cgcagtgccg gtgtcctggc aggtcggcag
     6361 cacgcctttg gcggcgattt cggagtttct taagaattgc agcgccacgt acttgtcgtt
     6421 ttcgctggct tctggatctt gaagaatagc cgcgacctgt ttctggtgtg ccgggcggag
     6481 catgaaagaa gcgtcgtgaa aggcttgctg cgccagcaga gtcagggctt ctggttccac
     6541 tttcaggatt gtttcgccgt cgaagtcggc aacgctaacg taatcggaag tgagtagata
     6601 gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg gtttgtttga
     6661 catagcttcc agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac
     6721 gggcggcacg caaagtgcat ttataagaac ccgtacatcg cggcgaagat ccagccgaag
     6781 acgcacgata cgctcacacc aatcaacccc ggcagaataa agctgtggtt gatgacgaag
     6841 cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg ctgccagatc gctcggataa
     6901 gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta tgccggatca
     6961 acgccgatcg ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg agagtttaca
     7021 aacttggaaa ccagcagcag aacaatggca taggcccacg gatactcttt caccatttca
     7081 cccagtacgc cctgaatttc agacatatgc gcaccgaaca tggtttcagc catccatgcg
     7141 ataccgtaca ccgccacgat ggcgatcata ccggaacgga agacttcgtt ttttgagata
     7201 gacgcgggat tggttttggt caggataata atcagcgccc cggtaagcag cataaacatc
     7261 tgaataacca gtaccatcga cagcggtttg ccaccgaagg atggacgcag gtccgaatca
     7321 gcaccaagaa gtgcgactac agcgattgcc ccgaggaaga tccacattgc aagccagttg
     7381 cttttcggca gttttttatc gagcagcgtc gcggtatcac cgtaaacata ctcacggttt
     7441 tccggtacgg agatgaattt ctggaactct tcgtctttat ccagatcttt accgcggaac
     7501 cagctgaaga taccgatcgc caggataccg attaacgtcg atggaatggt gattgccagc
     7561 aggtcgagga actcaagatg gcgaccatca aaggtgacat tacccagcat cgcaaccaga
     7621 gacacgactg caaccgacac cggactggcg ataatcccca tctgtgcacc gatagaactt
     7681 gccgccatcg ggcgttccgg acggatgttg ttcttaatgg cgacgtcgta gatgatcggc
     7741 agaatggtgt aaaccacatg acccgtaccg caaagaatgg tcagcgtaca ggtcacaaac
     7801 ggcgcgacaa ttgagacata tttcgggttg cggcgcagca gcttctcggc aatttgcagc
     7861 atgacatcaa gaccgcccga agcttgcaag gtcgccgatg ccgccaccac cgcaatgata
     7921 accagcatga catcaaccgg tggtttacct ggctgaaggt ggaagacgaa gaccagaatg
     7981 accagaccga taccgcctaa taaacccagc gcgataccac cctttctggc accataaaac
     8041 agacatatca gtattatgat aagttggata gtaaataaca tgtgtgaacc ctcgcgataa
     8101 tcctatttaa atttttgctg aatagatcac agtcacgttc tgttttgtat gaactgtttt
     8161 cagagcctga ctaaatatcc gtttggtcgt taccggcttt agcaaatacc tcacagtgaa
     8221 tattggctga ttaaagcagc ggcaaattta tgcactgtga taagcggctt tttcagagag
     8281 aaaaactctc tggcgatggg tttatagttt gtactgcgca gatgttaatg ttttgtttgc
     8341 tattatctta ttgatattta tcggtctatt ttttgttctc agggcagata tcattaggta
     8401 tatagattaa tctgatctac ccatttgtgg gtaaaaatac acataatgcg ggtgacataa
     8461 tagttaatta acttttgtta gcgttttgaa attaaaaaca ccgttcatct gaagagatat
     8521 taatttttaa cgatgatgga gggataatta tatttgatct ggcacaagtt ttactgatga
     8581 agaatgtaac ttgtgccagg aatattttgc attacagtaa taattattac ttacagataa
     8641 ccagcgatcc gttattggca atattgtttc agcagtgagt agtgttctgc ctgaatacgg
     8701 taacggtaaa ccggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag
     8761 ttgaccagcc agatgaggta tttacggcag gaaacacgcg aaatgttaac ctcgttagcc
     8821 agctcgtcgg ttgaaaactc atagtcctga tgcgcgtcaa tccactggca cagcgtgcgt
     8881 aacgtctgcg gcgttaagcc ttttggcaag cgacgaggat cctgctcgtt ggagctgctg
     8941 ccgtggatta gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt
     9001 ttcttttgcc gccagccggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc
     9061 aggtaatcca cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag
     9121 gagatgacaa tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc
     9181 ccgttctctt tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg
     9241 aagataatct ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg
     9301 atttgtgcta cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat
     9361 acattgatca tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga
     9421 aaatcccggg ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga
     9481 caagtgctaa accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt
     9541 gatcgatttt atcgggtgcg atccccggtc catcatcatt aacttcacag tgcagccagc
     9601 cgtgacggta gtgcaatgtt acgctaattt cgcctccggg ttccggccct aatgcttcca
     9661 gcgcgttttc tatcagattt cccaacgtgg taatcagcgt cgcgacctgg tcttcactgc
     9721 cgctgtctgg cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc
     9781 ggttaatctt gctgattaaa aaaccagcta taaccggaga tttgatctta cccagcagag
     9841 agccaatctc ttcctgatag ttattggctg ttttgagaat gtaatcttcc aactgcttat
     9901 aactcttcag atgcaataat ccgagaatca cgtgcaattt attcataaat tcgtgggatc
     9961 gttcacgaag tgcgtcagca tagttgacca gaccgtcgag tcgctgcatc agtttacgta
    10021 cttcagtttt gtctctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg
    10081 gaacggtgtt gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcgggg
    10141 taccgtcgcg taacacttcc gagacatcta ctacctgtga ccacgagtgg cttagcgtcg
    10201 acagtttctc atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga
    10261 tcagcgtgac ttcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca
    10321 tggcctggcg ttgctcaaac agcgtggaga tttcgtaggg ttccaggcca aaaagaattt
    10381 ttttcagtac tttaaccaga atgcaggtgc cgatcagtcc gaccagcatg ccaaataata
    10441 ccgaccagat aatgctccag cgactgtcat tgatctgttg ggtcacgcga cttaactcaa
    10501 ggccgatcgc caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc
    10561 gtaaagcctg cgccagaaaa ccgcgattga tagcgacatt ttcttcgcca ttcagcgctt
    10621 taaggatgtc atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc
    10681 gaagactttg catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt
    10741 ccgcgatggc ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg
    10801 gcgagtcggc gagggtacgc gccaccgcca gtgccttgtt ggctagcccg tctcgcgtca
    10861 tatcactgat ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg
    10921 cgctgaccat taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt
    10981 agggcaatga atgtctcatc agcttccttg tgtgacaaat ttcttaagca ttatctctga
    11041 tgaggcgggt aattcaaagg gagtaagaat gattggctat ataggggaag aggctcagga
    11101 aactgaaact gccggtgcgg tatgaagatt ccgggctatg cttattgcga aaaccatact
    11161 gatgagaggg ggaaggtcat ggatcaggat ctactggacg gtggttatcg ctgctatacc
    11221 ggcgaaaaga tcgatgtctg gttcaacact gcgatatgtc agcattctgg caattgcgta
    11281 cgtggcaacg gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtt
    11341 gacgtcacca ctgtggttaa agtgattgat acgtgcccga gcggcgcgct gaaataccgt
    11401 cataaataag cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg
    11461 acgaacaagg caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga
    11521 ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca ggggattggt aaacagctgg
    11581 ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat
    11641 ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga tattcgcagt tgatgggaga
    11701 gtacagagtc acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg
    11761 gtagggggat gcacaaagaa tgggcagaga gagcgttttt ttgtcccaag tcatcccctt
    11821 tactgagcaa aaaaaaaaga atatctccta tatgagaatc atcgattggg gttaataagt
    11881 tttgcgtccc cagagcgttt aaaattgata ggagtcatat tatggaaggt aaaaacaagt
    11941 tcaatactta tgttgtttct tttgattatc catcatctta ttcctcagtg ttcttaaggt
    12001 taagatcatt gatgtatgat atgaatttct cctctatcgt ggctgatgaa tatgggatac
    12061 cacgacaatt gaatgaaaat tccttcgcaa taacgacatc attagccgca agtgaaatcg
    12121 aagatttaat caggctcaaa tgcttagact taccggatat tgattttgac ctcaacatta
    12181 tgacagttga tgactatttc cgtcagtttt acaagtagcc aggaaggata agaagaacat
    12241 aaaaaatggc actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct
    12301 ttgtcattat ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc
    12361 tggtcggaat aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg
    12421 agctgtagtt gagtggaccc gctggttatc ttgaaacgaa gcgtctctcc tcaaaactgg
    12481 atttgcggaa cgacgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta
    12541 tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga atagtatggc tgtttgtaaa
    12601 cagcataatc attcggtcga taccaatacc cagaccagcg gttggcggca gaccatattc
    12661 cagcgcagtc acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cagctttagc
    12721 gttaacctgt tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc
    12781 attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ccgggttaac
    12841 atcattacgg cgcgccagcg gggacacttc tgccggatat tccgtaataa aggttggctg
    12901 aatcagatgt gcttctgcca cttcatcaaa gatctccgtc acaatacgtc ccagccccca
    12961 gcttttctct accgtaattc cgatagattc agctaatgct ttagcggcat caaaattatc
    13021 caggtcggcc atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt
    13081 ttcaaacggt ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtacccag
    13141 aacttcttgt gccagggtgc ggaacaggga ctccgtcagt tcaatcaaat cgtggtaatc
    13201 cgcatacgcc atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc
    13261 ttcattacgg aagttacggt tgatttcgaa tatccgttca aaaccgccta caaccagacg
    13321 tttcagatac agctccggcg cgatacgcag gtacatatct aaatccagag cgttatgatg
    13381 ggtaataaac gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac
    13441 ttccataaag ccgcgcgcga ccatgaactg acggatagcg gccagaattt ttgaacggac
    13501 aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg
    13561 gacttcctga tcctgcagac catggaattt atctggtaaa ggacgtagtg ctttagtcag
    13621 caggcgcagc tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa acagcgtacc
    13681 gcgggcaccg ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc
    13741 tggcaggcta tctcttgcaa cgtacagttg aatacggcca ccgacatcct gcaacgttac
    13801 aaaggaggct ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat
    13861 gtttaaggat tccagttcct ggttatcctt cgcatcaaac tcttcgtgca actggtcaga
    13921 ggtatggtcg cggcgaaaat cattaggaaa cgccacacct tgctgacgca gtgccgccag
    13981 tttttcgcgg cgatttctca gttcatcgtt aaaatcaata gcctcattgg ctccccgtgt
    14041 ttctcgttca gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag
    14101 acagatcggt acactgtatt atctgcctcg actattaata actcagttta tcttatcgaa
    14161 actaacgctt acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac
    14221 ttattaatct cgatttatta gtgtgataaa ctgaaagtta atttattcat aactaattgt
    14281 ttttacgaca gttaatcgtt gctctcctgt atcatattcg ttggcgtgct aaacagaaaa
    14341 cgggcagcaa aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatt
    14401 tgagaaaaga atcgctggta agcggcaatt gccataccgc taatttgcga ctctgtggtc
    14461 tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata
    14521 ccggttaata cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata
    14581 aagagttcgg caaagcccat tagcgccagc ccggatatca tcacgcccat tgacgcttga
    14641 ccgtcagccg ctgcatgtcg ggcatcaaat gccagcaaca taaagccaca agccatcagc
    14701 aataagccaa aggcaaactt cagccagacg cgcaatgttg agttgccgtg gctttctggc
    14761 gacgccagcc aggccagaac aaccccagcg agcatcaccg caattgcgtt cactgactgg
    14821 aatagtgcag taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac
    14881 aagctgatgg tactgccgcc ctgttgtgcc agtacccaga acaatgtccc gacaaacatc
    14941 aacaatacaa tttgccaaag agcacggcgg tgttcgggga atttgatcat catgcgggca
    15001 atgatttgtg cggcaatgag gcaaacgatc gccagcaaat atcctgacca gtcgttctcc
    15061 agcagcaggg taaaaaatac aggggctaaa cagagcatca ccactaacca gctccatact
    15121 ggtaaggcaa atttaacgct agtgagcgct tttttatcca tactacgcgt ggactggaaa
    15181 tgacgatgac cacttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca
    15241 aagccaacat gccatccata ccactgagca gccaggccgc aggcgatggg agcggcgata
    15301 gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc
    15361 tcgtcgtaga gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa
    15421 ataatgattg ccagcgccag atacaggcta aaagttgaat ttgtatcaat gcccagcacc
    15481 acatggccaa gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg
    15541 cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg
    15601 aacaggctga tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt
    15661 aaggcacgca tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat
    15721 atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg
    15781 gcaagccact tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac
    15841 ggtatagcgg ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata
    15901 gtgagcgccg atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt
    15961 gatcatttca cccggcatta ccagaggaac tcccggcgga tacggaagga tcatattggc
    16021 gttaatacga cctaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt
    16081 ctggaacgca gcatacggag tcattaccat ggtcggcaga acttcaaatg cgcgatacat
    16141 cagatccggc agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat
    16201 acgcatgttt tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac
    16261 acgcaggttc agatcgaacg cacgtttgaa gtctgtcaga gcacgcagca ggctcagtgc
    16321 tttggtctta tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc
    16381 aacaacgatg ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccgaagtc
    16441 gctcatggtg ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag
    16501 atacatgtgc tcgttatcga tgtttttgaa gccgtgccag ttgctgtcag aacgcagcgg
    16561 ccagcattca gtcgtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga
    16621 ttccgttctc agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt
    16681 gatcagacgc ttaccagcat tacctttcat catcgccgca gcggtttcag tggacgccac
    16741 gataccgtag tgcggagaag tggtggtgtg catcatgtag gcttcgttaa aggtttcttc
    16801 gtttacgtca cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg
    16861 agtagactgg gtttcgtaaa tcactttccc ttctacacga ccaccgctca taccgcattt
    16921 accttcgtaa atcggtgaga agttggtgta aggcacccac gcggagtcaa agtggatgga
    16981 tttcacatcc agtgttttct tgatgaagtc ggtattgtac agcagaccat cataggtaga
    17041 gttggtaatt acggcatgta ctggccaggt cgcgtttggt gtttctttca cgcgcttagc
    17101 aatggtagcg tgttggaatt cactctgtgg gataccacca agaataccgt aagcgttacg
    17161 agtcgggcgg aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt
    17221 gtggcagtta cggtcaatca gaatggtgct gcctgccgga gcagagtaca taccaacaat
    17281 tttgttcgca gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaatacgcg
    17341 agcgatatac tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc
    17401 agatactgaa atggaaatat cagatttcat ggtgttcgga ccaaagaaat catagaacag
    17461 gctacctacc gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt
    17521 atatttacct tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt
    17581 gatatattcg tcagtggtct gcttgatctt gttagcaata tcttcagcag cacccagcgc
    17641 atattcaaag aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata
    17701 cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag
    17761 ctcgagatta tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat
    17821 cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag
    17881 cgcgcgatga agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat
    17941 tgcaataacg ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca
    18001 aaatgaaagg aggagcctcg gaaaatactt ttaattaatg tgcgttagac gctgtgtggt
    18061 tatccattga gtggctctgg cgctcatgca ttttgcgagc gtagaacatc aggataatca
    18121 ggctgacgat gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc
    18181 agaacacgca acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt
    18241 caaaacgaat caggtcaacg caagagtaga agtacggcag catagtcagc agtactgcga
    18301 taccggtcag ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga
    18361 tgaggatcat cagggcagtc attttcactg cagccaacag cagacctttt ttcggaatac
    18421 cgttgctgtc gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac
    18481 ctgcctggcc taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga
    18541 atgcagaaac cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg
    18601 gagcaccgga agccgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag
    18661 cgatgtaaac aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt
    18721 tcgggttttt aaccatacca gtacttacag ctgcggattc aacacccacg aaggcccaca
    18781 ggcagagcag aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt
    18841 tagcagcata agttgccgca tcaaaccaat gccagccaac aatagctgtc attaccacag
    18901 gaataagaac cagcaccaga ccaatagtgg ttaaacggct tacccaggta ccgccgagca
    18961 tatttacaaa ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat
    19021 catttaatac tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt
    19081 taccaatcca gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggggaaa
    19141 tttctccggc ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat
    19201 atacatacgc cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac
    19261 caccgatact tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg
    19321 caacaacacc ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct
    19381 aatttcattt ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca
    19441 aactggcgct aagattactc acgaaaaaag gattaatcct aaagattagg tgaaataaca
    19501 caaaagtttc tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgcaagttg
    19561 atatcatgaa aagataaaca ttcaatgttt acaataaatt gcgggacatt atctggttaa
    19621 atttatgtaa taaaaattat gcggcaaata aattgccgca acatattata ccaacatgaa
    19681 catacaaaaa atcaacaaca aatattcccg agtatgaatc aacccgggag tcacttattc
    19741 tgaagcgaga aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc
    19801 attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaagcgg tgagatatgc
    19861 atcagctgct tcccggttca tccccttcat ttcataaacc ttgccaagca acacataatt
    19921 tagccaggac atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt
    19981 accttttacc agagcactga ccgcttttat ttgatatata atggacaggt tattcaattc
    20041 cggcagtgta acaatgttat ctatttctgt attcagtgct gctaattgtt tttcatctaa
    20101 aggatgttga gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa
    20161 ttctggggat gattgaacaa tctcacccaa taattcactg gcacggttca atgatttatc
    20221 atcgccatgc agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg
    20281 aggcaaaatt tgctggagca tctcctgcat tcgttgtggc cacggttggt ttaacgcttt
    20341 tgataaactc tccagtaaat cattttgaat cgccagctga ttaccgttag tgatgacata
    20401 acgtttatcc agcatggttg aaccatctgc attgtctacc aattttaccg acataaagca
    20461 ttgttgagca cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact
    20521 gctcggttca tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt
    20581 aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact
    20641 ccagctgtta caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat
    20701 acgcgatttg ctcatcggaa gacgtgtatc aagacttgaa aacgctacca gtgctacaca
    20761 gatacctaac gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt
    20821 aactggggat tgttcaggtg gcgttgcggt gttttgaatg ttaagactgt gggagggaga
    20881 atctgtggca ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc
    20941 ctcttcttcg ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt
    21001 agcgatatag acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat
    21061 actctgcgtc acaacgtgat tggtgacaat acttctcttc cagacattat cgataagttc
    21121 atccctgcta agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct
    21181 cggctcaagg gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag
    21241 ccattcgcca acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg
    21301 atgcaatgat aattgccaca acctattttt accatctata gatggtttta tttacatatt
    21361 attggtgaat gcaagacgtt atttttacca gccataaact tcttgattac atggtattac
    21421 gaaaggattt tactgagaac cagaagtaat attccttatc atcaaaattc atcatctttg
    21481 ccaaagaaaa atgttcataa aataatccat gggaaattgc ccggcgcgct tactattttt
    21541 atggattgtt agtctttgca tgagcaagcg gactgataca tttctctttg ttctcattca
    21601 gaaaatctca tcagtcggcg ttctgcccga ggttgtgctt tatggatgcg ttacgctcct
    21661 gatgacgtca tctgacgttc aacagcatca cggggccgcg cgacatttca cgtcagtcag
    21721 tgctatcgct caggaacaca tttccccgaa ttgggatatg cccgcaaatt gctggtgatg
    21781 tggggagaat ctggttgagt tcggtagaaa tgatttttag gcagaacgct taaattgtgg
    21841 cgtcctgaaa cgaaaaacgg acctccgtgg aggtccgttg atatgaattt ggtgcccgga
    21901 ctcggaatcg aaccaaggac acggggattt tcaatcccct gctctaccga ctgagctatc
    21961 cgggcaacgg ggcgcattaa accgtaatct gcacatctcg tcaacctaat ttcagtaaaa
    22021 gcgattcaac tgcttaagat tgcggcaaat cgcttctttt ctgtgtttct gtcaggtcaa
    22081 agcaccaccc tggcggcagc gggcgaagcg caggatatct tccgccagcc gatgtgcggt
    22141 gtcgacatcc gcctggctac gattcaccag cattcggctt aaacagcctt ccagcaccag
    22201 ttccatctgc tttgcaacca tcgccggatc gtcaacttcc agtgtggtta atagttcgtg
    22261 ggtgaaatcg taggccgcgc ttttttgctg atcggccagt tgatgaatag ggtggccagg
    22321 atcgggataa aacgtacagg cagcgataaa cagacagccc ggatagcggt tgtttttaac
    22381 gcactccgat aacgcctgat aacgtgccag caacttttgt tcggcggttt gcgtttcgtc
    22441 cagcatcagc tgacgacgcc agacatctat ctgttggcta agataacgca gcgcatcgta
    22501 gaggattgcc tctttgtctg gccagaagcg gcgtagctcg tccagtggat aatccacacg
    22561 ttcagcaacc atctccagcg tggtgttggc aatcccttgt aattctaata atttcagggc
    22621 ttctcccagt acatcttcac gttgcacgct attttcctcc gtctttccca ctgcaagtgt
    22681 cgttcacggt tggcgatcgc gcaaatgtgc gctgaaggtt tcagcatcca taaagcccgt
    22741 gacgcgtgct tgtggatgct cctggccttg tccgtcaaaa aagagaattg tcggtaggcc
    22801 aaggacatta agatgcttta acagcgccac atcttgtgcg tcgttggcag tgacgttggc
    22861 ctgaagtaat accgtgtctg ctaacgcttt ttgcacctgc gggtcgctga aggtgtattt
    22921 ctcaaactct ttacaggcga cgcaccagtc ggcatagaga tctaacatca ccggtttgcc
    22981 tttggcttca acgagcgcct gatttaactc atctaccgtt ttgatttgtg taaagttgag
    23041 atgcgtctga gtttgcgcgg tatgcgtcgc accaaatgcc caatcctgaa gtgggcgcac
    23101 gctaaccaat gccgctgcca gcaggattat ttgcaccacg cgcatccagc cgcgtttggc
    23161 ctgtaggctg gtgataaagg cccagccaaa gaatgcgaca ccaagcgccg accacaagcg
    23221 taatccccat atatcaccaa tcactcgctc cagcaggaag accggcagtg cgaggatcac
    23281 aaaaccaaac gcggttttga cttgttccat ccacgggccg cttttcggca gcaggcggtt
    23341 accaaagacg gttattagca tcagcggcag gcccatgccc aacgcataga gataaagcgt
    23401 gccgcccccc agcaacatgt tcccgctttg ggcgatatac agcagaatcg cgctaagcgg
    23461 tgcggtggtg catggtgaac agatcagtcc ggcaatcgcc cccataacaa acacaccgcc
    23521 aggtgagccg ccctgttggc gattgctcat cagcgtgagg cgcgtttgca gcgaagaggg
    23581 gagttgcagg gtaaacaagc caaacattga catcgccagc aaggtaaaga cgatagcgag
    23641 gccaatgagc acgtatgggt gctgtagcgc cgcctggaac tgtaaccctg cggcggcaac
    23701 caccagaccc agtgccgtgt aagtcagcgc catcccctgc acataaataa aggtcagcaa
    23761 caatgctctg gcagtggaaa gccgctgttt accgcccagc acgatgccag aaatcagtgg
    23821 gtacattggc agcacgcatg gcgtaaaggc gataccaata ccgatcaaca acgcccagag
    23881 cgcggaaaag ggcaattgcg cggtgggctg ctcttgctgc ggaacagaca caggctgtga
    23941 cgctgcgttg ttggcgacca cttcgcttaa cggaacggtt ttggtttctg gcggataaca
    24001 gaaaccggca tcagcacagc cctggtaggt gacagttaac gtcgctcccg cactcgcctg
    24061 gttgatggtt acgggaagcg tcagccgatc gcggtaaatc tcgcttttgc cgtaaaactc
    24121 atcttcatgc cagacgcctt gcggcagctg cacgtcggca attttcgcgt gttccggcgt
    24181 aatgcggacc tgtttacggt agaggtagta accgtctttg atctgccagg tcagattcag
    24241 gtcatgttgg ttttgctgaa aatcaaaagc aaaggcttga tccgcgagga caaattgtga
    24301 acgtcccggc gcgtcgaata atccggcaaa aacggaagtg ctgcaaagta gcaggatcag
    24361 cgtaaagatg cgttgagcca tgagaggtaa tctgtgtctc cgtgtgtaac aggtaaaacc
    24421 agaagttccg gggtttgata tggatgatga gacttcaggc attccagcag tgcctgctgg
    24481 tgagatacgg tagtttttaa aatcatctgc acttcgtatt cttgctccag cttaccttcc
    24541 cagtaataga gagaggtagc gccggggatc aaggtcgcgc aggccgccag tttttccgcc
    24601 agcactttgg cggctaaatc ctgggctgtc gcttcatccg gtgccgtaca tagcaccacg
    24661 acagacgcgg tattcgaact tttttcatca agcataaaca cctcgcaaga acagatggaa
    24721 ccgcaagaga gaggtcacta tacaacggac gggggaagga tgttagtcgg cgggaaagaa
    24781 agcggggcct gaagccccgc gattggtcat tacagcatga agctacccag cacgaagccg
    24841 aagcaaacgg ccagggcaac acccagagta cccgggatga agaacggatg gttgaagacg
    24901 aatttaccga tacgggtagt acccgtgtca tccatctgta ccgcagcaac cagcgtcggg
    24961 taggtcggca gaatgaacag accagacacc gcagcgaaag aagcaacagc ggtcagcgga
    25021 gaaacgttca gtgccagagc catcggcatc agtgcttttg cggttgcagc ctgagagtac
    25081 agcagagcag acgcaaagaa gaagatgacg gccagcagcc acggatgacc ctgaatcact
    25141 tcaccagcgg tatctttgat ccagtcgatg ttgttggaaa cgaaagtatc gcccagccat
    25201 gcaacaccca ggatacaaat acaggcgctc atacctgctt tgaaggtgct ggagttgagg
    25261 atgttgtcgg tatcgacttt acagataacg gtggtcagag ttgcaacgct gagcatgatg
    25321 atcaggattg cgttggtggt gttcatcagc ggtttttcaa ccagacccat gcttgggctg
    25381 ttgatgattg catagataac aacgccaact acgcccagca ggaacagcca gacggacgtt
    25441 tttgcaccgg atttgatttc aatctgcttt tcaccgcgca gttcaaccag gccctcttcc
    25501 agacgcttgc gataaatcgg atcgtcagag agtttggagt tgaacagcat agtgaccagg
    25561 aaggacatca ccagaaccgc cagcagggtg gacgggatga ccacggagag cagatggagg
    25621 tagctgatgc catgaccttc cattacggaa gacatgtaaa ccactgccgc tgagattggc
    25681 gatgcggtga tcgcaatctg cgcggatacc actgcagtag acagcggacg gcaaggttta
    25741 acgccttgtt ccttcgcaac ttcagcgata actggcagtg tcgccagaga gatgttgcca
    25801 gtaccagcaa agattgtcag gaaataggtc acgatcggtg cgaggatcgt gatgtatttc
    25861 gggttacggc gcagcagctt ttctgtctga tgaaccagat agtccagacc gccagcaacc
    25921 tgcatggcag aaatagcggc gataaccgcc atgataatgg aaatgacatc gaacgggatg
    25981 ttaccgggtt taacgccaat agcggcaaga accagcaccc ccaatccgcc tgcaaaacca
    26041 atacctattc cccccaatct ggcgcccaag aagatcgcca gcaaaactat gatgagttct
    26101 acaactagca tattagcctt ccttgttttt taacaagttg atattagatt gttattttta
    26161 agttactgct cacaagaaaa aaggcacgtc atctgacgtg ccttttttat ttgtactacc
    26221 ctgtacgatt actgttcgct ttcatcagta tagcgttttg ctttgtaagc cgggtgcatc
    26281 agattctgta cggagaaaat atcgtcaagt tccgcttcag tcaacagacc gcgttccaga
    26341 acgacttcac gtacactctt accggtttcg gcacagattt tacccacgat gtcaccgttg
    26401 tggtgaccga tgaacgggtt caggtaagta acgataccga tagagttgta aacgtaacct
    26461 tcgcacactt ctttgttagc agtgatgccg ttaatgcatt tttccagcag gttgtagcaa
    26521 gcgttggtca gaatgtgaac ggattcaaac atggcctggc caatgaccgg ctccataacg
    26581 ttcaactgca gctgacctgc ttctgctgcc atggtaacag tggtgtcgtt accgatgact
    26641 ttgaagcata cctggttaac cacttccgga acaaccgggt ttactttagc tggcatgatg
    26701 gaagagcccg cctgcagttc cggcaggttg atctcgttca ggccggcacg tgggccagaa
    26761 gagagcaagc gcaggtcgtt acagattttg gacatcttca cagccaggcg tttcagcgcg
    26821 ccgtgaacca taacataagc gccgcagtca gaggtcgctt cgatcaggtc ttcagccggt
    26881 acgcatggga agccagtgac ttcagccagt tttttcactg ccagcggaga gtactctttc
    26941 ggcgtgttca gaccagtacc gattgctgtt gcgccaaggt taacttccag cagcagttca
    27001 gcggtacgtt ggatattttt cacttcttct ttcagcagga tgctgaaagc gcggaattcc
    27061 tgaccgaggg tcatcggtac tgcgtcctgc agctgggtac gacccatttt caggatgtcc
    27121 tggaattcga cagctttacg ttcaaagcct tcacgcagtt ggttaatcgc atctaccagc
    27181 ttaatcaggg aagagtaaac tgcgatacgg aaaccggtcg ggtaggcgtc gttagtggac
    27241 tgacatttgt taacatggtc gttcgggttc aggtactgat attcaccttt ctggtgaccc
    27301 atcagttcca gaccgatatt ggccagcact tcgttggtgt tcatgtttac ggaagtacct
    27361 gcgccgccct ggtagacgtc taccgggaac tgatccatgc attttccgtt gttcaggact
    27421 tcatcacatg cggcaatgat ggcattcgct acacttttag gaatggtttg cagctctttg
    27481 tttgccatag ctgcggcttt tttaaccatt accataccgc gaacaaattc aggaatatca
    27541 ctgattttgt tgttgctgat atagaagttt tcaatcgctc tcagagtgtg aacaccatag
    27601 taggcatcag ctggaacttc cctggtaccc aacagatctt cttcgatacg aatgttgttt
    27661 gacatgtgaa ccttcttttt caagctgcca atgatttgct ttaaacacac agaatatatg
    27721 tggtttcgaa tgtttttcga ccgacgatta tcccctgcat cgaccgaata cccgagatca
    27781 tatgctgctt gaggatttct accgtaatct ggatcacttt aagtgtcggt ttttacccct
    27841 taattattaa tttgtgaaat agatcaccgc tttgggatta ctaccaaaaa tagttgcgca
    27901 aacatcttga aattttgcta atgaccacaa tataagctaa acgcgattcg caacccattc
    27961 aggtagccgg ggttaaccgg ctgctattac aggagaaacc tttgcgctgg ttacctttta
    28021 ttgccatttt cctttatgtc tatattgaga tttcaatctt tattcaggtt gcccatgtat
    28081 tgggggtatt gctgaccctc gtgctggtta tattcacgtc agttatcggt atgtcactgg
    28141 tacgtaacca gggctttaag aatttcgtgc tgatgcagca aaaaatggcg gcgggtgaaa
    28201 acccagcggc ggagatgatt aaaagtgttt cgctgatcat tgccggtttg ctgcttttat
    28261 taccgggctt tttcaccgac ttcctcggtc ttctgctttt attaccgccg gtgcaaaagc
    28321 atctgacagt gaagttgatg ccgcatttgc gcttttctcg catgccaggc ggcggtttta
    28381 gcgccgggac cggtggcggt aatacttttg atggtgagta ccagcgaaag gatgatgacc
    28441 gcgaccgcct tgatcataaa gacgatcgcc aggattaatg ccgaaacgcc ggattatgtg
    28501 gttatgccat tttccggcgt ttttcgtttt ggcagaaaca gccataaccc cgccagcatg
    28561 atcagcgcat agagactttt ccagccgacc attgccagta acagaacgca taacagcccg
    28621 ccaaccaccg ccagtagtcg ataacgtcct tgcaataatt tacagcctgc cagcatgcat
    28681 aacagataaa tcataataaa gatgccattg gcatagatga taagtgcgtc cagattgatc
    28741 cctaaagcat gaatcaccaa cgtgctcacc acgcagcagc cgagcaccgc attgagggca
    28801 ttattcggga tatgacgaga agagaggcgt gccaggtagt ggtcaggatt atgttgcgcc
    28861 tgcgaccaga ccaggcgggc gaagctctgt atataaatgt tgagactggc aaagcaggcc
    28921 agatagccaa tcacgcaggc aatccataac gctcctacac cgaacagttg cactacaatt
    28981 tttggaagcg atgctgctgc cgccatttgt tcaccatagg cgtcgaagtg taagacgact
    29041 accgtacagc cccagtagac taatcctgcc agcagcagac caatcatcaa agcacgagga
    29101 aaatcacgct ctggattttt aaattccgag gcaagatggg caaatgcttc cagaccgaca
    29161 aagcaccaga acatcactga taacgcagca aataacccgg taagttcgat attaccaggt
    29221 gcagggaagg ggatattcgc aggtttgata tcgcccgccc accagatagc gacaatcagc
    29281 gcgacgataa gtccggcaat aactgtttgt agattagcac tggaactggc accgcgagta
    29341 ccgatatacc acaccagcgc cagcgtaccg agttctgcca acaacagttg ccagctatgc
    29401 cagccaaaca ttgcctggcc gaatccggct gcaatttgta gtgcggcagg caaacccacg
    29461 ggaatgactg ataaaaacag ccagccggtg actcgctcaa gccgcgaacc aaacgccata
    29521 ccgacgaagt gcgcgacgcc gcctgcgctg ggatagtggc gacccagaat cgcaaacaca
    29581 atcgcaatcg ggaacactaa gataatcaaa acgggccacg cccacaggct gttattgccc
    29641 gctaccagcg cagctaacgc aggaacggca aacacgccag tgcctaataa tgacgtcgat
    29701 agcaggccaa tgccctgggc cagtcccagt tcttgtttga gtccactcat gggttgatgt
    29761 ccgattgcgc ccaaattatg ggcaactgcg tagattttcg atggtagcac aatcagattc
    29821 gcttatgacg gtgatgaaga aattgcgatg aaatgtgagg tgaatcaggg ttttcacccg
    29881 attttgtgct gatcagaatt ttttttcttt ttcccccttg aaggggcgaa gcctcatccc
    29941 catttctctg gtcaccagcc gggaaaccac gtaagctccg gcgtcaccca taacagatac
    30001 ggactttctc aaaggagagt tatcaatgaa tattcgtcca ttgcatgatc gcgtgatcgt
    30061 caagcgtaaa gaagttgaaa ctaaatctgc tggcggcatc gttctgaccg gctctgcagc
    30121 ggctaaatcc acccgcggcg aagtgctggc tgtcggcaat ggccgtatcc ttgaaaatgg
    30181 cgaagtgaag ccgctggatg tgaaagttgg cgacatcgtt attttcaacg atggctacgg
    30241 tgtgaaatct gagaagatcg acaatgaaga agtgttgatc atgtccgaaa gcgacattct
    30301 ggcaattgtt gaagcgtaat ccgcgcacga cactgaacat acgaatttaa ggaataaaga
    30361 taatggcagc taaagacgta aaattcggta acgacgctcg tgtgaaaatg ctgcgcggcg
    30421 taaacgtact ggcagatgca gtgaaagtta ccctcggtcc gaaaggccgt aacgtagttc
    30481 tggataaatc tttcggtgca ccgaccatca ccaaagatgg tgtttccgtt gctcgtgaaa
    30541 tcgaactgga agataagttc gaaaacatgg gtgcgcagat ggtgaaagaa gttgcctcta
    30601 aagcgaacga cgctgcaggc gacggtacca ccactgcaac cgtactggct caggctatca
    30661 tcactgaagg tctgaaagct gttgctgcgg gcatgaaccc gatggacctg aaacgtggta
    30721 tcgacaaagc tgttaccgct gcagttgaag aactgaaagc gctgtccgta ccgtgctctg
    30781 actctaaagc gattgctcag gttggtacca tctccgctaa ctccgacgaa accgtaggta
    30841 aactgatcgc tgaagcgatg gacaaagtcg gtaaagaagg cgttatcacc gttgaagacg
    30901 gtaccggtct gcaggacgaa ctggacgtgg ttgaaggtat gcagttcgac cgtggctacc
    30961 tgtctcctta cttcatcaac aagccggaaa ctggcgcagt agaactggaa agcccgttca
    31021 tcctgctggc tgacaagaaa atctccaaca tccgcgaaat gctgccggtt ctggaagctg
    31081 ttgccaaagc aggcaaaccg ctgctgatca tcgctgaaga tgtagaaggc gaagcgctgg
    31141 caactctggt tgttaacacc atgcgtggca tcgtgaaagt tgctgcggtt aaagctccgg
    31201 gcttcggcga tcgtcgtaaa gctatgctgc aggatatcgc aaccctgact ggcggtaccg
    31261 taatctctga agagatcggt atggagctgg aaaaagcaac cctggaagac ctgggtcagg
    31321 ctaaacgtgt tgtgatcaac aaagacacta ccaccatcat cgatggcgtg ggtgaagaag
    31381 ctgcaatcca gggccgtgtt gctcagatcc gtcagcagat tgaagaagca acttctgact
    31441 acgaccgtga aaaactgcag gagcgcgtag cgaaactggc aggcggcgtt gcagttatca
    31501 aagtaggtgc tgctaccgaa gttgaaatga aagagaaaaa agcacgcgtt gaagacgccc
    31561 tgcacgcgac ccgtgctgcg gtagaagaag gcgtggttgc tggtggtggt gttgcgctga
    31621 tccgcgtagc gtctaaactg gctgacctgc gtggtcagaa cgaagaccag aacgtgggta
    31681 tcaaagttgc actgcgtgca atggaagctc cgctgcgtca gatcgtcctg aactgcggcg
    31741 aagaaccgtc tgttgttgct aacaccgtta aaggcggcga cggtaactac ggttacaacg
    31801 cagcaaccga agaatacggc aacatgatcg acatgggtat cctggatcca accaaagtaa
    31861 cccgttctgc tctgcagtac gcggcttctg tggctggcct gatgatcacc accgagtgca
    31921 tggttaccga cctgccgaaa aacgatgcag ctgacttagg cgctgctggc ggtatgggcg
    31981 gcatgggtgg catgggcggc atgatgtaat tgccctgcac ctcgcagaaa taaacaaacc
    32041 cccgggcaga aatgtctggg ggtttttctt ttggtcatct ttctagtata agattcagac
    32101 acggacgacg cgagtggcgt ccagctcatt gattatgggg aataacatgc acgtaaaata
    32161 cttagcaggg attgtcggtg ccgcgctact gatggcgggt tgtagctcca gcaacgaatt
    32221 gagtgctgcc ggtcagagtg tacgcattgt ggacgagcag ccaggcgcag agtgccagct
    32281 gattggtact gcgacaggta agcaaagcaa ctggctttcc gggcaacacg gtgaagaggg
    32341 cggttctatg cgcggcgcag caaacgatct gcgcaaccag gcggctgcaa tgggcggtaa
    32401 cgtgatttat ggcatcagca gcccgtcgca ggggatgttg tccagttttg tcccgacgga
    32461 tagccagatt atcggccagg tatataagtg cccgaactga tgggatccag aggctggata
    32521 cttccagcct cgtagtaaaa cagatgggat tatttcgcct gtggtgcagt tttgctggtg
    32581 gattgaacac gtaagtgatg cagcgcgcgt aagcattgat cctgggttag cggttgggcg
    32641 ttatttgccg ctacggtgcg aacatagacc tgacatggca cacccgcgag ttttttcagt
    32701 cgggggctga aatcaagcat tcggcgagtg atcgcctcca cttctttacc cggcgttgct
    32761 tcacgcgttt tcaccactgc gccgctaaat tgttctttgc cactttcatc tttaaagcgg
    32821 taaagcaccg ccagataatg gttgaacaag ttatgtttcc attccggttg ggaataggtg
    32881 tcatattcca gattgccatt gtcctggcag tcaacgtcgt ataacggcag gaaatctaac
    32941 agtgaggtaa tacgtaatgc cagttcacgc atttccgcat tattaattgc atgaatgatt
    33001 gctcgcgcca gcatttcaat ctgcaattca ttgatgacta actcacattt gctaccgtca
    33061 tgtagcgtta atacgaaggt aagattttca ccctgattat cggtaagttc cagcgtgtta
    33121 acccgacgat taatatcggc atttttaagc tcatcaacca gaatttctgg gatattttct
    33181 gccattttct taatgacttt atcgcgagct tgctcatact gaaggcgtgc agcagcatcc
    33241 agtttatgct tttgatgcag acggctttcc agcgcaatca gaaggtcgcg cagttccatg
    33301 acagacagaa agaacaacga ttctttattg cgaggctctt taattttcag cgccagtgca
    33361 atgaaattat tactcttg
//
LOCUS       DARNVM010000035        32746 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.035, whole genome shotgun sequence.
ACCESSION   DARNVM010000035 DARNVM010000000
VERSION     DARNVM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.035"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(386..1327)
                     /gene="fabY"
                     /locus_tag="SI621_001529"
                     /gene_synonym="yiiD"
     CDS             complement(386..1327)
                     /gene="fabY"
                     /locus_tag="SI621_001529"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HEI4064260.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1372..1809)
                     /gene="dtd"
                     /locus_tag="SI621_001530"
     CDS             complement(1372..1809)
                     /gene="dtd"
                     /locus_tag="SI621_001530"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEI4064261.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1806..2678)
                     /locus_tag="SI621_001531"
     CDS             complement(1806..2678)
                     /locus_tag="SI621_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HEI4064262.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSMQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2672..3271)
                     /gene="yihX"
                     /locus_tag="SI621_001532"
     CDS             complement(2672..3271)
                     /gene="yihX"
                     /locus_tag="SI621_001532"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HEI4064263.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3370..4155)
                     /locus_tag="SI621_001533"
     CDS             complement(3370..4155)
                     /locus_tag="SI621_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4064264.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4189..5085)
                     /gene="yihV"
                     /locus_tag="SI621_001534"
     CDS             complement(4189..5085)
                     /gene="yihV"
                     /locus_tag="SI621_001534"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HEI4064265.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGNLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5253..6149
                     /gene="yihU"
                     /locus_tag="SI621_001535"
     CDS             5253..6149
                     /gene="yihU"
                     /locus_tag="SI621_001535"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HEI4064266.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6173..7051
                     /gene="yihT"
                     /locus_tag="SI621_001536"
     CDS             6173..7051
                     /gene="yihT"
                     /locus_tag="SI621_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HEI4064267.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRRINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7067..8308
                     /gene="yihS"
                     /locus_tag="SI621_001537"
     CDS             7067..8308
                     /gene="yihS"
                     /locus_tag="SI621_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HEI4064268.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYAIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8376..9278
                     /locus_tag="SI621_001538"
     CDS             8376..9278
                     /locus_tag="SI621_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HEI4064269.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQDYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDDYLFQLPAEQVYAVDEHANPTTLHHVDELNLNFTRATKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            9339..10214
                     /locus_tag="SI621_001539"
     CDS             9339..10214
                     /locus_tag="SI621_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEI4064270.1"
                     /translation="MDFFTLFSPDGAMVETSVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLPRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRNWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            10322..12358
                     /gene="yihQ"
                     /locus_tag="SI621_001540"
     CDS             10322..12358
                     /gene="yihQ"
                     /locus_tag="SI621_001540"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HEI4064271.1"
                     /translation="MDTPRPQLPDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTDSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEQGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            12404..13789
                     /locus_tag="SI621_001541"
     CDS             12404..13789
                     /locus_tag="SI621_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064272.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQIGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTE"
     gene            13830..15233
                     /locus_tag="SI621_001542"
     CDS             13830..15233
                     /locus_tag="SI621_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064273.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     SGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15300..15992
                     /gene="ompL"
                     /locus_tag="SI621_001543"
     CDS             15300..15992
                     /gene="ompL"
                     /locus_tag="SI621_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HEI4064274.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(16084..17349)
                     /locus_tag="SI621_001544"
     CDS             complement(16084..17349)
                     /locus_tag="SI621_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064275.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17451..18431)
                     /locus_tag="SI621_001545"
     CDS             complement(17451..18431)
                     /locus_tag="SI621_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEI4064276.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(18439..19149)
                     /locus_tag="SI621_001546"
     CDS             complement(18439..19149)
                     /locus_tag="SI621_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEI4064277.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KKRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19366..21189)
                     /gene="typA"
                     /locus_tag="SI621_001547"
     CDS             complement(19366..21189)
                     /gene="typA"
                     /locus_tag="SI621_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HEI4064278.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21562..22971
                     /gene="glnA"
                     /locus_tag="SI621_001548"
     CDS             21562..22971
                     /gene="glnA"
                     /locus_tag="SI621_001548"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HEI4064279.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            23145..24194
                     /gene="glnL"
                     /locus_tag="SI621_001549"
     CDS             23145..24194
                     /gene="glnL"
                     /locus_tag="SI621_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEI4064280.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            24206..25615
                     /gene="glnG"
                     /locus_tag="SI621_001550"
     CDS             24206..25615
                     /gene="glnG"
                     /locus_tag="SI621_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEI4064281.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25727..25837
                     /locus_tag="SI621_001551"
     CDS             25727..25837
                     /locus_tag="SI621_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HEI4064282.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(26027..27400)
                     /gene="hemN"
                     /locus_tag="SI621_001552"
     CDS             complement(26027..27400)
                     /gene="hemN"
                     /locus_tag="SI621_001552"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEI4064283.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFILALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKHYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27589..28098)
                     /gene="yihI"
                     /locus_tag="SI621_001553"
     CDS             complement(27589..28098)
                     /gene="yihI"
                     /locus_tag="SI621_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HEI4064284.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            28680..29312
                     /locus_tag="SI621_001554"
     CDS             28680..29312
                     /locus_tag="SI621_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEI4064285.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(29694..32480)
                     /gene="polA"
                     /locus_tag="SI621_001555"
     CDS             complement(29694..32480)
                     /gene="polA"
                     /locus_tag="SI621_001555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEI4064286.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLVEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7933 a         8454 c         8085 g         8274 t
ORIGIN      
        1 acatgccaca taattattta caacatccag aagcagtaaa tgaatgatac tgacaatgac
       61 gccagtccat tatctttgca taatgtttgt tagctataga ttttaaaaat atttatgaga
      121 aaagtatggc accagagggg gttttgacta tcaaaaatcg ctcaaacctg aactaagatg
      181 atgaattaag cattatcagc gataattgcc actcagctct acaaataata tttaaatgct
      241 cctgaacagt gatgtagcaa gacacgtatg gtgccaacga agaataaaaa tttatagagt
      301 tcttattcaa tataaccgga atagtcagag actgaagtaa atgacctgaa agaatgagag
      361 aggcagcacc gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc
      421 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
      481 cgccaaagat ttcgacctgc atttgtactc gcgctttgcg cccgcgcgcc agacggtcga
      541 gatcgccgct taaggcaccg aggtcggcta ccgcatgagg tttaccgcta atcggcttgc
      601 tgtagcgaat atgcgcatcc gccagaataa tcgttccgcc cagatggcgt tcacgcagca
      661 tcagccagat aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca
      721 gcgtatggtg cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg
      781 tatattgctg aatgcgcacg cccatttttt cactcagcgg gatatgttcg taccacgcct
      841 gttgcagctg cgcgcaccag tcgccgcgat gcagaatgtc atccagagtg gcgacaggtt
      901 ttatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatctcc ccttgattga
      961 caaaccccag cttggcgaaa aactccaccg cgtcttcgcg ggcgctacag gtcacgcgct
     1021 taacaccttc ctgacgcgcc acagattcga gggtcatcgc catcagcgtt cctaaccctt
     1081 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
     1141 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     1201 ccatcgcatc ccacgcgtcg cgttccgaac ctttcggttg atgcaggggc ttacgcaaca
     1261 tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt
     1321 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     1381 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg cagcgaagcg
     1441 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     1501 ctctgcgcga tccggtgatg cacctttgga gaaacttgga cgcatccccc gttcggtatc
     1561 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     1621 gttcatcttg ccttcggcat cgctaaagat gcgatagccg agcacacgct cgcacagacg
     1681 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     1741 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     1801 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     1861 ccgagagtga cagtaatttc cgcaccaagc aagacgatac accatgtcca gtagacccaa
     1921 acaaagagaa tggggatcac cgccagcaca ccgtaaatga gctgatatga ggggaacatg
     1981 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagtgcggc gacaaacgcg
     2041 ccgacaatcg cgtcgcgatt aggtacgcgg atagtaggaa caatgctgta cagcaaccag
     2101 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttgtcgat gacagtattg
     2161 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     2221 agcagcggcc ccagcgttaa aatcatccag tacacggcaa acgagtaaat ttttggtcgc
     2281 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     2341 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     2401 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     2461 tgacgcaact gcatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     2521 acggcaacaa gcggcactaa tgagagcaac gaaacatagg caaggttacc tgccagggtt
     2581 gtcatgttgt cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg
     2641 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     2701 ggatggtggt tttatctttc accagaatac tggtaatgcc tagctgattc gctccttcta
     2761 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     2821 aaacatgctg ataaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     2881 tatggtcagc agcatcacgg atttccgggt attcctccgg ccagaaggta gtatgcaggc
     2941 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     3001 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgggag aactgctcat
     3061 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     3121 ccccacgctc atgctgatga aacgcctcgc ccatgtggaa actcttctta agcgtagcca
     3181 gtggaacacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa
     3241 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     3301 aactgtagcg ggaaaggatg ggtttggcta tgtggtcagg tatgaagcaa ggtcaacggt
     3361 cacaaaattc tacgcgtctt cctggggaag gatgatttca atttggctgt cttgtaagcg
     3421 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcggtaacct gtgccacgtt
     3481 accaatttca accgccgccg aagcatgata cttagtgtga tcggcgacca gcaggatatt
     3541 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg ttaacatcaa actccatcaa
     3601 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc
     3661 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     3721 gggcaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     3781 ggtgattatc cgcaaatgat tgtggttaag taacgcccgg gcaacatgtt caacagtcgt
     3841 accgatggtg ataaatattg tcgatccatc aggaatatag tctgccaccg cttcggcaat
     3901 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     3961 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagt ccttgctcgc ttaatttacg
     4021 aatatcccgg cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     4081 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     4141 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc cgggcatttt atacaaaaag
     4201 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgcgt
     4261 gcattttaac gccgccacac cgctggcgaa gcgaactgac tccgctaaat tcccaccggt
     4321 tgccaatgcc accgccagcg caccgtgaaa aacatcaccc gcaccggtgg tatctaccac
     4381 atcaactttg aaagccggtt gatgttgacg cccgccattt tccagccagt cgcagccggc
     4441 actcccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     4501 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc
     4561 gcttaatgcc accagctcgc tgatatcctg cggcgtaatg tccccgtcca gaacggtcat
     4621 cacgcccgcc tgccgggcca gagtgaaggc ttttttagca ccgtcgtgcc agcgtacatc
     4681 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     4741 cagatccggg ctggggtagt taatgattat ccgctcgcct ttggcatcca ccatgatggc
     4801 ggattgcgaa gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga
     4861 ttccagctct gccagcaggc tgttgcccgt gtcgtcatca ccgacgcgac caataaaatc
     4921 gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     4981 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaatcctt ccacgtaata
     5041 aatacgatcc atcacggtaa tacctacaca agcaacacga atcatggtca ttccttaagc
     5101 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     5161 tttgacgata aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca
     5221 aaaagtgaca tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag
     5281 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct
     5341 ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca
     5401 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     5461 tggtgcgcag cgtgttgttc ggtgaaaacg gcgtttgcga aagcttatct accgatgcgc
     5521 tggtcatcga tatgtcgacc atccacccgc tgcaaaccga taaattgatt gccgatatgc
     5581 aagccaaagg cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta
     5641 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgc gccacaccga
     5701 tcctgatggc gatgggcagt gagttgatca acgcgggcgg tccgggcatg gggatccgcg
     5761 ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgctg
     5821 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaggtgatg agcggcaccg
     5881 ccgccggtaa aggccacttc accacttcct ggccaaacaa agtcctcagc ggggatcttt
     5941 ctcccgcctt catgatcgat cttgcccata aggatcttgg tatcgccctt gatgtcgcca
     6001 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     6061 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     6121 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     6181 gtacaccatc aacgacatta cacgcgcatc gggcggtttt gccatgctgg cggtcgatca
     6241 acgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccag tagccgatag
     6301 cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat
     6361 tctggtggat cagcaattct gttaccgcca ggtggttgag caaaacgcga ttgccaaaag
     6421 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtagatag
     6481 cgtggtcatt gaccgcagaa tcaatccgct acaaatcaaa caagacggtg gcaaagcctt
     6541 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa
     6601 agagtttaac gaactgtgcc actcacacgg cctggtaagc attattgagc cagtcgtccg
     6661 cccaccacgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     6721 actgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc
     6781 gcaacaagag cttctcagtg cttcacaacg tctgaatgac catatcaata tgccgtgggt
     6841 gatcctctca tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     6901 agcgggtgca tcgggcttcc tggctggtcg tgccgtctgg gcatcggttg tcggtttacc
     6961 agacaacgag ctgatgctgc gtgacgtttg cgcacctaaa ttacaacaac ttggcgatat
     7021 cgtcgacgaa atgatggcta aacgccgtta agatggggat acaaaaatga aatggtttaa
     7081 caccttaagt cacaaccgtt ggctggaaca ggaaaccgac cgcatctttg attttggtaa
     7141 aaattccgta gtaccgaccg gatttggctg gttaggcaat aaagggcaaa tcaaagaaga
     7201 gatgggcacc catctgtgga tcaccgctcg tatgttgcac gtttattccg ttgctgcggc
     7261 gatgggtcga cctggcgctt actcgttggt tgatcacggt atcaaagcca tgaacggcgc
     7321 actgcgcgat aaaaaatatg gcggttggta tgcctgcgtg aatgacgagg gcgtggtgga
     7381 tgcctccaaa cagggctatc aacacttctt tgctctgctg ggtgctgcca gcgccgtcac
     7441 taccggtcac ccggaagccc gtaaacttct cgattacgcc attgaaatta ttgagaaata
     7501 cttctggagc gaagaagagc agatgtgcct ggagtcctgg gacgaagcct tcagcaaaac
     7561 cgaagagtac cgcggcggca atgccaacat gcacgcggtg gaagccttcc tgattgtcta
     7621 tgacgtcacc catgacaaaa aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat
     7681 ccacgacgtc gccagaaata atcattatcg cgttaacgag cacttcgata cccagtggaa
     7741 tccgctgccg gattacaaca aagataaccc ggcgcaccgc ttccgcgcat tcggtggcac
     7801 gccgggccac tggatcgaat ggggccgttt aatgctgcac atccacgcgg ccctggaagc
     7861 ccgttgtgaa cagccgcctg cctggctgct agaagatgcc aaaggtctat ttaacgccac
     7921 cgtgcgcgat gcctgggcac ccgatggcgc ggacgggatt gtttataccg ttgactggga
     7981 aggcaaaccg gtggttcgcg aacgtgtgcg ttggcctatc gtcgaagcga tgggtacggc
     8041 ttacgcgctc tacaccgtca ccggcgatcg ccagtatgaa acctggtatc agacatggtg
     8101 ggactactgc attaagtacc tgatggacta tgaaaatggt tcctggtggc aggagctgga
     8161 tgcggacaat aaagtcacca cgaaagtctg ggacggcaaa caggatattt atcacctgct
     8221 gcattgcctg gtgatcccgc gtatcccgtt agcccctggt ctggcaccgg ctgttgccgc
     8281 aggcctgctg gacattaacg cgaaataact gagctgaaat aacggcatat caggctacat
     8341 gccgggtatg tcgttattca ataaaggtgc ctcgtatgca aatcaccaac atgcattgca
     8401 gtggacaaac cgtttcactt gccgcggggg attatcacgc gactatcgtg acggttggtg
     8461 ctggtctggc tgaattaacc tttcagggtt gtcatttagt catcccgcac aaacccgaag
     8521 agatgccgct ggcgcatctg ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg
     8581 gctgttaccg ctatcagggg caggactatc agttacccat taatgaacat agttcaaaag
     8641 ccgctattca cgggctgctt gcctggcgtg actggcaaat tagcgaatta accgccacca
     8701 gcgttaccct gacagctttt ctgccgccca gttacggcta tccgtttatg ctggcttcgc
     8761 aggttgttta ttcgctcaac gctcgcacgg gcctttccgt tgagatcgca agccagaata
     8821 ttggtactgt gaccgcacct tatggtgtgg gtattcatcc ttatttaacc tgcaacttaa
     8881 catctgttga tgactatctt ttccagcttc cggcagaaca ggtttatgcc gtcgatgagc
     8941 acgcaaatcc gacaacgctt caccacgttg atgagctgaa tttaaatttc acgcgggcga
     9001 caaaaatagc cgcgacaaaa atagaccata cctttaaaac cgctaatgat ctgtgggaaa
     9061 taaccattac ccatccacaa caggcgcttt cggtgagcct ctgttccgat caaccctggg
     9121 tgcaggttta tagcggtgaa aaattacagc gtcagggact ggcagttgaa cctatgagtt
     9181 gcccgcctaa cgcctttaat tcaggtatcg acttattatt actggaacca ggaaaaacgc
     9241 accgcctgtt ttttaatatt cacgggcagc acaattaatt ttattaagaa aagaacgaga
     9301 tcattgttta gggttgtatt tacacaacag gaaatattat ggactttttt acgttatttt
     9361 cgccggatgg ggccatggtg gaaacaagcg tggtatgtat cgttatcgcg ttaatgtttc
     9421 tttatacctt tgtggggata tgcgcgggat ttggcggtgg tttaaccact atgccgctcg
     9481 tcacgttaat gctccccgta aaaatggcaa ctccgctctc ggtgattgtc ggcaccgcga
     9541 ccgccttgta cgccacctgg ctgccacgta aagaaacgga ttggcgatcg gcaatggtac
     9601 tgattatttt ctcgtttctg ggaatacccg tcggtttata tgcgctgtcg tatttacccg
     9661 accattttat gaaagtgggc ttaggcggat tccttatcct ttactcgttt tacagcatgt
     9721 ttattcctcg cctgccgatt tatgaccgta actggatcgc cgcacctatc ggcgcagtag
     9781 ctggcgcatt aggcgcggcg ttctcaacca acggaccgcc gatggttatc tacggtatgc
     9841 tgcgtaactt aggcccggcg gcatttcgtg gcactctgaa cgctttcttc accgcaaaca
     9901 acatcgcggt gatcggcggg ctggcgacca gcggaatttt gactgtttct atctttaaac
     9961 tggtgctgtt ttgtattccc accatgatcc tcggttcact ggttggacaa tatgtgcata
    10021 aacgcatttc agtcaaagtg ttccgtgtga tggtcttttt gttgctgatt gcctccggtt
    10081 ctatgttgat aaaaggcgca ctgggaattc ccgccgctgc cgcacttctg cctccgttta
    10141 ccttgttagt tatactgcaa ctggcatttg gtaaaaaagt ggcggcaatc cgccgggctg
    10201 aagaaagtaa ataatcactt atgcgggttt taatttatac ccgcacacat tctttaatta
    10261 atgaaacctg taacaacagg atggcatgaa atattagctt tatactctat ggagattatt
    10321 tatggatacg ccacgcccac aattaccaga ttttcagttt catcagaata acgacagttt
    10381 taccctacat tttcaacagc gtcttatttt aacccatagc aaagataatc cttgtttatg
    10441 gattggttca ggtattgcgg atatcgatat gttccgcggc aatttcagca ttaaagataa
    10501 actacaggag aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggttggtt
    10561 aatccatttc agccgtggtt ctgacattag cgccacgctg cgtatctctg ctgacgatca
    10621 gggccgttta ttgctggaac tacaaaacga caaccttaac cacaaccgca tctggctgcg
    10681 ccttgccgct caaccagagg accatatcta cggctgcggc gaacagtttt cctacttcga
    10741 tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca
    10801 aacctatgtc acctggcagg ccgactgcaa agaaaatgcg ggcggcgact attactggac
    10861 tttcttccca cagcctacgt ttgtcagcac gcagaagtat tactgccatg ttgataacag
    10921 ttgctacatg aacttcgact ttagcgcccc ggaataccat gaactggcgc tgtgggaaga
    10981 caaagctacg ctacgttttg aatgtgctga cacatacatc tccctgctgg aaaaattaac
    11041 tgccctgctg ggacgccagc cagaactgcc cgactggatt tatgacgggg taacgctcgg
    11101 cattcagggt gggacggaag tgtgtcagaa gaaactggac accatgcgta acgcgggtgt
    11161 gaaggttaac ggtatctggg cgcaggactg gtccgggatc cgtatgacct cttttggcaa
    11221 acgcgtaatg tggaactgga agtggaacag cgaaaactat ccgcaactgg actcacgtat
    11281 taagcagtgg aacaaagagg gcgttcagtt cctggcctat atcaacccat atgtcgccag
    11341 cgataaagat ctctgcgaag aagcggcaca acacggctat ctggcaaaag atgcctctgg
    11401 cggtgactat ctggtggagt ttggcgagtt ttacggcggc gttgtcgatc tcactaatcc
    11461 ggaagcctac gcctggttca aggaagtgat caaaaagaac atgattgaac tcggctgcgg
    11521 cggctggatg gctgacttcg gcgagtatct gcccaccgac acttacctgc ataacggcgt
    11581 cagcgcggaa attatgcata acgcctggcc cgcactgtgg gcgaaatgta actacgaagc
    11641 ccttgaagaa acgggcaagc ttggcgagat ccttttcttt atgcgcgccg gttctaccga
    11701 tagccagaaa tactccacta tgatgtgggc gggcgaccag aacgtcgact ggagtctcga
    11761 cgatggcctg gcgtcggtcg tcccggcagc gctgtcgctg gcaatgaccg gacatggttt
    11821 gcatcacagt gacattggcg gttataccac cctgtttgag atgaagcgta gcaaagagct
    11881 gctgctgcgc tggtgtgatt tcagcgcctt cacgccgatg atgcgcaccc acgaaggcaa
    11941 ccgcccgggc gacaactggc agtttgacgg cgacgcggaa actattgccc attttgcccg
    12001 tatgaccacc gtcttcacca ccctgaaacc ttacctgaaa gaggccgtcg cgctgaatgc
    12061 gaagtccggc ctgccggtta tgcgcccgct gttcctgcat tacgaagacg atgcgcagac
    12121 ctacaccctg aaatatcagt acctgttagg ccgcgacatt ctggtcgctc cggtgcatga
    12181 acaaggccgt agcgactgga cgctctatct tccggaggat aactgggtcc acgcctggac
    12241 aggtgaagcg ttccggggcg gggaagtgac cgttaatgcg cccatcggta aaccgccggt
    12301 cttttatcgc gccgatagcg aatgggcggc actgttcgcg tcgttaaaaa gcatctaatc
    12361 acgtccgccc gtggggaaac tcacgggcaa agggagaaca acgatgagtc acatcacaac
    12421 ggaagatccg gctactttac gtctgccctt taaagagaaa ctctcttacg gtatcggcga
    12481 tctggcctct aacatcctgc tggatatcgg cacgctttat cttttgaagt tttataccga
    12541 cgttctgggg ctgcccggca cctatggcgg cattatcttt ttgatctcga aattctttac
    12601 cgcctttacc gatatgggta ccggcatcat gctcgattcc cggcgcaaga tcggtccaaa
    12661 aggcaaattc cgccctttta ttctgtatgc atcattcccg gtcactctgc tggcgatcgc
    12721 caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg gtgatggcca ctattctgtt
    12781 tatgctctac gggctgtttt tcagcatgat gaactgctcc tacggcgcaa tggtccccgc
    12841 tattaccaaa aaccccaacg aacgcgcatc gctggcagca tggcgtcagg gtggcgctac
    12901 attgggctta ctgctgtgta cggtgggatt cgtgccggtt atgaatctta tcgaaggtaa
    12961 tcagcaaatt ggctatatct tcgctgccac gctgttttca ctgttcggcc tgctgtttat
    13021 gtggatctgc tactcgggcg tgaaagagcg ttatgtcgaa acccaaccta ccaatccggc
    13081 gcaaaagcca ggcctgttgc aatctttccg cgcaattgcc ggtaaccgcc cgctgttcat
    13141 tctgtgcatt gccaacctct gcactttagg ggcgtttaac gtcaagctcg ccatccaggt
    13201 ctattacacc cagtacgtac ttaacgatcc catcctgttg tcgtatatgg gatttttcag
    13261 catgggctgt attttcatcg gcgtgttcct gatgcctggc gcagtcagac gttttggtaa
    13321 gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg ggcgatctgc tcaactattt
    13381 cttcggcggc ggctcggtca gctttgtggc gttctcctgc ctggcattct tcggctcggc
    13441 gtttgttaac agcctgaact gggcgctggt ttccgacacc gtcgagtacg gcgagtggcg
    13501 caccggtgtg cgttcggaag gtacggtcta caccggcttc accttctttc gcaaagtatc
    13561 tcaggcgctg gcaggtttct tcccaggctg gatgctgacg caaatcggct atgtgccaaa
    13621 cgtcgcccag gctgaccata ccatcgaagg tttacgccag ctgatcttca tctacccaag
    13681 cgcactggcg gtagtcacca ttgttgcgat gggttgtttc tacagtctga acgagaaaat
    13741 gtacgtccgc attgttgaag aaatagaagc ccgtaaacgc acggaataaa taataaacaa
    13801 cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg acattaaaac
    13861 tgaatctgcg ggaaaaaatc gcctatggca tgggcgacgt cggttcgaat ttaatgctta
    13921 gcatcggcac tctgtatctc ctcaaatttt ataccgatga attagggatg cctgcttact
    13981 atggcgggat tatctttctg gtagcgaagt tttttaccgc gtttaccgat atgctcaccg
    14041 gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt ccctttattt
    14101 tatatgcagc ggttccggcg gcgttaattg caacgctcca gtttatcgcc accaccttta
    14161 gtttgccggt taaaacgacg attgccacag cactgtttat gatgtttgga ctttcataca
    14221 gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac cccaatgagc
    14281 gcgcgcagct cgcagcttac cgccagggtg gtgcaaccat agggctattg atttgtaccg
    14341 tggcgtttat tccgttgcag tcgctttttt ctgactccac cgtcggttat gcctgcgcgg
    14401 cactgatgtt ctccattggt ggctttattt ttatgatgct gtgctacaga ggcgtcaaag
    14461 agcattatgt ggacacgacg ccaagcggac ataaagccag tattctcaaa tctttttgcg
    14521 cgatatttcg taatccgcca ttactggttt tgtgcattgc taacctgtgt accctggcgg
    14581 catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg aatgatatta
    14641 atttattgtc gtggatggga tttttcagta tgggatgtat cctcgtcggc gtattactgg
    14701 tgccattaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc atggtgctat
    14761 gggcggttgg tgatatcctg aattatttct ggggaagtaa ctctttcact ttcgtcatgt
    14821 tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg gcactggtgc
    14881 cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt tctgtttata
    14941 ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg ccaggcatta
    15001 tgctgacgca aatcggttat gttcccaaca tcgcgcaaag tgatgcgaca ttacagggtt
    15061 tgcgtcagct catttttatc tggccttgtg cgctggcaat tattgctgca ttaacgatgg
    15121 gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa atcaaccaac
    15181 gcaaaaataa agagatggcg acagaagaaa aaacagcttc cgtaacgtta taaatgtcaa
    15241 tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaat ggacttatta
    15301 tgaaaaagat taatgcaata attctattat cctctctgac ctctgcctcg gtatttgctg
    15361 gcgcatacgt cgaaaacagg gaggcatata atctcgcctc cgatcagggc gaagttatgc
    15421 tacgcgtggg ttataacttc gatatgggcg cgggtattat gttaaccaat acctacacct
    15481 ttcagcgaga agatgaactc aaacatggct ataatgaaat tgaaggttgg tatcctttat
    15541 ttaaaccaac cgataaatta accatccagc ccggtggctt aattaatgac aagagtatcg
    15601 gttcgggtgg tgccgtttat ctggatgtta actacaaatt tactccgtgg tttaacctga
    15661 cggtgcgtaa tcgctataac cataataatt acagctctac agatttgaac ggcgaattgg
    15721 ataataacga cacctatgaa gttggcacct actggaattt taaaatcacc gagaaattct
    15781 cctatacatt tgaaccacat tacttcatgc gagtaaatga ctttaatagc agcaatggaa
    15841 aagatcatca ctgggaaatc actaacacct tccgttatcg cattaatgaa cactggctcc
    15901 cttatttcga attacgctgg ttagatcgta atgtcgaacc gtaccaccgc gagcaaaacc
    15961 agattcgtat cgggacgaag tatttcttct gatgtaaaac tgccggatgc ggcgaaaacg
    16021 ccttatccgg cctacggttg atacggtttc cgttggccgg attaagccac atccggccat
    16081 taattaagct tcagccgcca gcatctcttt cttacgttgg ctaatacgct taaccagcat
    16141 tacggaaacc accgcaccac agaatgcgaa gcaggccata atgccaaaca cgattttgta
    16201 gccaataatc cccggattta aatccagaat gtagccatac agactgaagc agaacatcgc
    16261 cggggcgtaa ccaataaagc tacccaacgc catcgccgcg cctgttttat tttcagcaat
    16321 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
    16381 gcccagcgta catgccatcc ctaaatatac cggcatactt tcgtgcggca gcataatcaa
    16441 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
    16501 caggatctta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
    16561 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
    16621 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
    16681 aagccagata gttttgtctt tcagcaccga ggtcatactg gtgtttttcg atgctccgtc
    16741 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcagaacaa agaaaatgat
    16801 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacccaataa
    16861 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
    16921 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
    16981 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
    17041 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
    17101 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
    17161 agacagaaaa aagcccactg tcgtgacaaa tgagtttacc gacatagcat taccaatttg
    17221 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
    17281 taatttataa attgtaccgc cacacaatgt taatagacta aataacgccc atttcttttt
    17341 cgtgagcata acatccccta agcgattttt aaaatcactg ttttaaaggc aataagatac
    17401 cgaccattca tatggaatgg ttttattaac tgcgtatgag ttaaaactta ttagtggttc
    17461 agaagatggt ttgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
    17521 ttaattgcat cttctttttc tttacgtaac cgcgcatcca gtaaatgatt ttctggtagg
    17581 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
    17641 aacgccggcg catagtaatc aacctcttct tccaggccat atgccgtcac ctgcggctca
    17701 tcatcaccca ggcagataag gtgcgttaat aacgctttga acggcatatc accctgacct
    17761 gaaatacagg ctttatgacc caggccaccc tcttctttaa cgatcaaggc atctttgata
    17821 tggacctggg taatatgcgg tgccatcgtt tttaaagcgt cgatgggatg ctcatttgca
    17881 ttgatcatgt tcgcaaaatc aaacagtaag gaaagagatt ccatctcact ttctttgacc
    17941 agcgacacca gctcatggct ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg
    18001 ctgtcctgat acgtttcccg tacataggca atgtcgttag cgatgatcga taatacgtcg
    18061 cgcaaattac cttcataacg tggataaaaa cgtacggacg atgccccagt tttcaacgca
    18121 atggcgacgg cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca
    18181 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
    18241 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt
    18301 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
    18361 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
    18421 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
    18481 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
    18541 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
    18601 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
    18661 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
    18721 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
    18781 agttgatggt gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacgttt
    18841 tttctcggta aacccccagg aaggctctcg tccttgctca attgcggccc gctgaaagct
    18901 cgctgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
    18961 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
    19021 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
    19081 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
    19141 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
    19201 aaaagaaagc aattaacggt gtatgacttt gcagataatt gtgacaaagc tctctattga
    19261 cgtaatcaca gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa
    19321 aaaacctgcc agcgatggca ggttttttat gactgaaaag cgaaattaat cgtctttcgg
    19381 tgcgcggttg gcgcggcgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
    19441 ggtcggagtc acttctacca gttcgtcatc atcgataaac tccagagctt gttccagagt
    19501 catgcggata ggcggaacca gaacaacggc ttcgtcagta ccggaagcac gcatgttggt
    19561 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
    19621 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcag
    19681 accgaacaac gcgaacgcta ccgctttacc ctgaccgtta gagatcagta cgccgttctg
    19741 acgctgaccc acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag
    19801 acccgtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
    19861 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
    19921 tttacgttcg cccagcgcct gcatcacaga accctgatgc tgttcttcaa cgtccagcgt
    19981 cacgttttca tacggctctt gtttacgacc gtcgatttca cggaagatta ctttcggacg
    20041 ggataccgcc agttcgaaac cttcacgacg catgttttcg ataagaacag acaggtgcag
    20101 ttcgccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
    20161 aacgttgtgt accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt
    20221 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
    20281 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
    20341 gttcagttcg cccaggcccg tgatcgcaac gatatcgcca gcttccgcca gatcggtttc
    20401 aatacgttcc agaccgaggt ggcccagcac tttaccgact ttcgcgttac gggttttgcc
    20461 ttcgctatcg atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc
    20521 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
    20581 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctgat acagcggggt
    20641 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
    20701 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
    20761 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
    20821 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
    20881 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
    20941 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
    21001 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
    21061 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
    21121 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
    21181 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
    21241 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
    21301 tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa
    21361 tgtttccatt gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac
    21421 gatggtgcgc atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt
    21481 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga
    21541 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
    21601 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca gcacgtcact
    21661 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
    21721 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
    21781 accgcagtga ttgatccgtt cttcgccgat tctaccctga ttatccgttg cgacatcctt
    21841 gaacctggta ccctgcaagg ctatgaccgt gacccgcgct ccatcgcgaa gcgcgctgaa
    21901 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
    21961 ttcctgttcg atgacatccg tttcggatct tctatctccg gttcccacgt tgctatcgac
    22021 gatatcgaag gcgcatggaa ctcctccacc caatatgaag gtggtaacaa aggtcaccgt
    22081 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
    22141 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
    22201 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
    22261 gacgaaattc agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc
    22321 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
    22381 tcgctgtcta aaaacggcgt caacctgttc gcaggcgaca aatacgcagg tctgtctgag
    22441 caggcgctgt actatattgg cggcgtaatc aaacacgcga aagcgattaa tgccctggca
    22501 aacccgacca ccaactctta caagcgtctg gtcccgggct acgaagcacc ggtaatgctg
    22561 gcttactctg cacgcaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
    22621 gcacgtcgta tcgaagtacg tttcccggat ccggcggcta acccgtacct gtgctttgct
    22681 gccctgctga tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg
    22741 gacaaaaacc tgtatgacct gccgccggaa gaagcgaaag agatcccaca ggttgctggt
    22801 tctctggaag aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc
    22861 gtgttcactg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
    22921 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
    22981 ttgtactacc cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct
    23041 ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag
    23101 ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc
    23161 agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta atcgatgaca
    23221 acctggcgat ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca
    23281 aattgtttgg tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga
    23341 tgcaagaaag tctggaggcg gggcaaggtt ttactgataa cgaagtgacg ctggtcatcg
    23401 acgggcgttc gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc
    23461 tggagatggc accgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc
    23521 agcaggttgc tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg
    23581 gcggtttacg tggcgcggcg cagctgctca gcaaagcatt acctgacccg tcactactcg
    23641 aatataccaa agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt
    23701 tgggaccgca actgcccggt acgcgcgtta ccgaaagtat tcataaagtg gctgaacgcg
    23761 tggtgacgct ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc
    23821 ccagcctgcc ggaactggcg cacgacccgg atcaaattga gcaagtgctc ctgaatattg
    23881 tgcgcaatgc gctacaggcg ctggggccag agggtggtga aatcattctg cgtacccgca
    23941 cagctttcca actgacctta cacggcgagc gttatcggct ggcggcgcga attgatgtgg
    24001 aagataacgg gccaggtatt ccgcctcatt tgcaggatac actgttttac ccgatggtca
    24061 gcggccgcga aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc
    24121 attcaggcaa aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc
    24181 ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga
    24241 tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacaac
    24301 atttgagaac ggcgcggagg tactggaggc gctggcgagc aaaacgccgg atgtgctgct
    24361 ttcagatatc cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg
    24421 ccatccgatg cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag
    24481 cgcctatcaa caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagccgt
    24541 ggcgctggtt gagcgcgcca tcagccatta tcaggaacag cagcagccgc gtaatgttca
    24601 gcttaacggc ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg
    24661 tattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac
    24721 cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgagagcca aagcgccatt
    24781 tatcgcgctg aatatggcgg ctatcccgaa ggatttgatc gaatcagaac tgtttggtca
    24841 cgagaaaggc gcgtttaccg gcgcgaatac cattcgtcag gggcgttttg aacaggccga
    24901 tggcggcacg ttatttctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt
    24961 gctgcgcgtg ctggcagacg gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt
    25021 ggatgtgcgg attatcgctg ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa
    25081 gttccgtgag gatctgttcc accgcctgaa cgtcatccgc gttcatctgc caccactgcg
    25141 tgagcgtcgg gaagatattc cccgcctggc acgccatttt ttacaggttg ccgcgcgcga
    25201 actgggcgta gaagcgaagt tactgcatcc ggaaaccgaa gccgcgctga cgcgtctggc
    25261 gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg taatggctgc
    25321 cggacaggaa gtgttgattc aggatttgcc tggcgaactg tttgaatcaa cggttgcgga
    25381 gagtacttcg caaatgcaac cggacagttg ggcgacgctg ttagcgcagt gggcagacag
    25441 ggcgctgcgt tccggtcatc aaaatctgct ttccgaagcg cagcctgagc tggagcggac
    25501 gttactgacc accgcgctgc gacatacgca ggggcataaa caggaagcgg cgcggctact
    25561 cggctggggg cgtaacactc tgacgcggaa gttaaaagag ctggggatgg agtgattcac
    25621 ggcttgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt
    25681 ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat
    25741 aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc
    25801 cgtgctgtgt gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc
    25861 tctttgttca tgccggatgc ggcgtgaacg ccttatccgg tctacaaaag caatattttc
    25921 aacaggtttt gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg
    25981 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgag
    26041 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
    26101 ggatcagcaa gcgacctttc gccgtcacct gaatcccctt ctcatccaca tccaccagcc
    26161 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
    26221 aatcccactg ttgctcaatg ggagcgtaat ccagacggaa gttgcagatg agcgacttaa
    26281 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
    26341 cttgttcatc cacttgctga tagtagtgct tcaactcttt ctggttctgc gcatagcagt
    26401 cgccaatcat actgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
    26461 tgtagccctg gaagttacgg tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
    26521 ccggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
    26581 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
    26641 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg
    26701 ggttcagctc cgccacgcgt ttcagggtaa aagcgaaact ctccggcgtt tgtttcggca
    26761 ggccgtaaat caggtcgatg ttggtggagg taaagccaat ctcacgcgca tggttaagca
    26821 gtgccaagat gaattcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
    26881 agtcctgcac gcccatactc agacgattaa agccctcggc gcgtaaatga tcgagtacat
    26941 ccagttcgat ttcgcgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga
    27001 agttttcgcg cagcagcttc atcagacggc tgatttgcgc tttgttcaga tacgtcggcg
    27061 taccaccgcc ccagtgcagc tggctcacct tgcgcccggc aaaaagcggt gcacgatgga
    27121 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
    27181 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
    27241 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
    27301 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
    27361 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
    27421 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
    27481 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
    27541 aaccggaagg gttatcagca gaacgggaag acccacggcg gcaatccgtt agttgccccg
    27601 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
    27661 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttggcat ccacccagga
    27721 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
    27781 ctcatccgtt tccagtaact ccaactccgc ctgcggtgaa agcataggtt tctcactctt
    27841 cggtttgtgc tgtttggtga ctttttcagt cacgcccaat ggaatagggg ttttactgcc
    27901 aatacgtgga tctttcggtg cgttctggcc tttactgcct gacgtggtgt taccgcccgc
    27961 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttacggt cacgagcttc
    28021 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
    28081 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
    28141 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    28201 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    28261 tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    28321 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    28381 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    28441 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattaactg
    28501 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    28561 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    28621 ccttttatta tggttcaggt ataatcgccg caaagctttg actgacggag acgaccgctt
    28681 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    28741 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    28801 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    28861 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacctgcccg
    28921 gctatggcta cgcggaagtc ccggaagaga tgaagcgcaa atggcaacgc gcgctcggcg
    28981 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    29041 cgctgaaaga tttggatcaa cagatgattg agtgggcggt agacagcaat atcgccgtgc
    29101 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    29161 tggtgcgtga agcggtgctg gcgtttaacg gtgatgtgca ggttgaaacg ttctcttcac
    29221 tgaagaaaca aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc
    29281 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    29341 gtggcgtatc cggcccgtaa attcaataga ttacacttct accgttggcc tgataaacat
    29401 ggcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    29461 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    29521 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    29581 aagcattttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    29641 acgtgacagc ttatgttgct tacttacgaa aaaagggcat attcaggcga atcttagtgc
    29701 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    29761 cagttttcca tcagttgatg aatctgcttc gcgacggcat cgacatcatc tttatgaact
    29821 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    29881 agccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    29941 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag ccccattgct ggatttgata
    30001 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    30061 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    30121 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    30181 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggttacggtt
    30241 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    30301 aatgcggtca gcaagccttt gtcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    30361 gagtagtccg ccgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    30421 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    30481 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    30541 atcagcggca gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga
    30601 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    30661 ggcgcgccac ccggcgtttt cttcagcggt ttaattccct gtttttcaaa cagaatggtt
    30721 tgcaactgct tggtggagga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    30781 tccagctcaa ccagacgaag agtgagctct tcagaatgat tgtgcagcac tttcggatcg
    30841 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ctagcggcat ttcgatattc
    30901 tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt cagatgcagc
    30961 tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    31021 ttaaaggtca gttgattttt acctttacca gcaatctctt caaaagtgat ggttttatgc
    31081 ttcaaccaac gttccgcgag gctgtccata tcgtggcgac cggcaacgct gttgagaatg
    31141 taggactcca gcatggtatc aaatgcaatc ccccgcagtt caatgccgta gttcgccaga
    31201 ataccgcgat cgtatttcag gttttgccca accttcagcg ccttttcatc ttccagcagc
    31261 ggttttagca actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca
    31321 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    31381 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    31441 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca
    31501 taagaaatca ccgttgccgt cacttctggt gcttcgtctg caacactggt ttcctgtggc
    31561 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    31621 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    31681 acttccagtt gttcgcaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg
    31741 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    31801 cggaagctca acccggcaat tttttctggc tcggcataca gcgtatccag tccgccaaga
    31861 ccttgcagca atgcctgtgc ggttttttca ccgacgcccg gtacgcctgg aatgttatca
    31921 gaggagtcac ccatcagcgc caggaagtcg atgatcaatt ctggcggcac gccgtactta
    31981 ttcaccacct cttccggtcc gaggatggta ttcgtcatag tgttgataag cgtaatattt
    32041 ggcgtcacca gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct
    32101 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    32161 agcagcggca gtcccatcgc tttaaccatc gcgtgcaaag gttcgatttg tgcacgcaga
    32221 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    32281 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    32341 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    32401 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    32461 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    32521 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    32581 tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    32641 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgaagttaaa
    32701 aataagacca tccctaataa ttgagataat gggatttttt ctaatg
//
LOCUS       DARNVM010000036        32529 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.036, whole genome shotgun sequence.
ACCESSION   DARNVM010000036 DARNVM010000000
VERSION     DARNVM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.036"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1353)
                     /gene="ccp"
                     /locus_tag="SI621_001556"
     CDS             complement(<1..1353)
                     /gene="ccp"
                     /locus_tag="SI621_001556"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEI4064287.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPDHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     H"
     gene            1758..3407
                     /locus_tag="SI621_001557"
     CDS             1758..3407
                     /locus_tag="SI621_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HEI4064288.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3458..4060)
                     /locus_tag="SI621_001558"
     CDS             complement(3458..4060)
                     /locus_tag="SI621_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEI4064289.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4581..5480
                     /locus_tag="SI621_001559"
     CDS             4581..5480
                     /locus_tag="SI621_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4064290.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGASPEDGVIARPLGK
                     LTVVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLIYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5529..6542
                     /gene="yhjD"
                     /locus_tag="SI621_001560"
     CDS             5529..6542
                     /gene="yhjD"
                     /locus_tag="SI621_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HEI4064291.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
     gene            6934..8256
                     /locus_tag="SI621_001561"
     CDS             6934..8256
                     /locus_tag="SI621_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HEI4064292.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8438..10498)
                     /locus_tag="SI621_001562"
     CDS             complement(8438..10498)
                     /locus_tag="SI621_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEI4064293.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHIHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDGNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10568..11335)
                     /gene="pdeH"
                     /locus_tag="SI621_001563"
     CDS             complement(10568..11335)
                     /gene="pdeH"
                     /locus_tag="SI621_001563"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HEI4064294.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLSPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11567..12496
                     /gene="kdgK"
                     /locus_tag="SI621_001564"
     CDS             11567..12496
                     /gene="kdgK"
                     /locus_tag="SI621_001564"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HEI4064295.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12592..14088)
                     /locus_tag="SI621_001565"
     CDS             complement(12592..14088)
                     /locus_tag="SI621_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEI4064296.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLSSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14308..15594)
                     /gene="dctA"
                     /locus_tag="SI621_001566"
     CDS             complement(14308..15594)
                     /gene="dctA"
                     /locus_tag="SI621_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HEI4064297.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15777..17765)
                     /gene="hmsP"
                     /locus_tag="SI621_001567"
     CDS             complement(15777..17765)
                     /gene="hmsP"
                     /locus_tag="SI621_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HEI4064298.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMNSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17847..21320)
                     /gene="bcsC"
                     /locus_tag="SI621_001568"
     CDS             complement(17847..21320)
                     /gene="bcsC"
                     /locus_tag="SI621_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HEI4064299.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQWQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNQLIPLAKSQPPSMESAMVLRDGAKFEARAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKIINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21302..22408)
                     /gene="bcsZ"
                     /locus_tag="SI621_001569"
     CDS             complement(21302..22408)
                     /gene="bcsZ"
                     /locus_tag="SI621_001569"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HEI4064300.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22415..24754)
                     /gene="bcsB"
                     /locus_tag="SI621_001570"
     CDS             complement(22415..24754)
                     /gene="bcsB"
                     /locus_tag="SI621_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEI4064301.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24765..27383)
                     /gene="bcsA"
                     /locus_tag="SI621_001571"
     CDS             complement(24765..27383)
                     /gene="bcsA"
                     /locus_tag="SI621_001571"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEI4064302.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27380..28132)
                     /gene="bcsQ"
                     /locus_tag="SI621_001572"
     CDS             complement(27380..28132)
                     /gene="bcsQ"
                     /locus_tag="SI621_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HEI4064303.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDDQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDSSQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            complement(28144..28332)
                     /locus_tag="SI621_001573"
     CDS             complement(28144..28332)
                     /locus_tag="SI621_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HEI4064304.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28604..30175
                     /gene="bcsE"
                     /locus_tag="SI621_001574"
     CDS             28604..30175
                     /gene="bcsE"
                     /locus_tag="SI621_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HEI4064305.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEGITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQNSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30172..30363
                     /gene="bcsF"
                     /locus_tag="SI621_001575"
     CDS             30172..30363
                     /gene="bcsF"
                     /locus_tag="SI621_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HEI4064306.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30360..32039
                     /gene="bcsG"
                     /locus_tag="SI621_001576"
     CDS             30360..32039
                     /gene="bcsG"
                     /locus_tag="SI621_001576"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HEI4064307.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32126..32233)
                     /locus_tag="SI621_001577"
     CDS             complement(32126..32233)
                     /locus_tag="SI621_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEI4064308.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIMNWLNKRK"
BASE COUNT         7655 a         8906 c         8197 g         7771 t
ORIGIN      
        1 gtgcaggaaa gctacgatat catccacatc ctcctgcggc agctctttgc ctacctgata
       61 gcgcaacatc agtttcaccg ccccgtccag cgtcggcaca tcaccgcgat ggaagtacgg
      121 tgccgttagt gcaacgttac gtaaaccggg tactttctga cgcaatttat cgcgctcttc
      181 tttagtaaca ttcatacgac caatatccgc cgccgtaatt tcaccaaagt taaagtcttt
      241 tttcagcccc aacggttcga aggaacgtcc gccgagaata ataccaccat gacaagttgc
      301 acatttatta tctttgaata attgatagcc ttttttctgt tgtgccgtca gcgcattctc
      361 atctccacgc aaccatttat caaatgggga atccggcgta attaatgttt tctcaaattc
      421 agcaatggca tcagtaatat tttcgccact gaaaccttgc ggatagactt caaggaactg
      481 cgctttaagc tgcggatctt tttccagctt agcaataatt tcgtcccagg atttcgacgc
      541 catttcaatc ggattcaacg gcggtccacc agcctgatcc tgcaatgttg ctgcacgacc
      601 atcccagaac tgctcaacgt taaatactga gttaaatacc gtcggcgcgt taatcggccc
      661 cactgcgcca ccaacaccaa tcgatgtttt tctgccatcg acgccccccg cattcaacgc
      721 atggcaatga gcgcatgaaa tggtgctatc cgccgataaa cggggatcgt gatacagcgc
      781 aaagcccaac gccacttttt gcgcatcggt aggcagtttt tgcgggatgg gctgcaccgg
      841 ttcattacga tgatccggtg cagtgtcatt gctggcgtaa tattccgcgc gctgttttgc
      901 aatccaggcg agtatttccg cccgctcttc atcactcacc ttacccgccc agtgtagcgc
      961 ggtataacgc gttggtggca tggtttcata ctgcatcacc cattcaatct tattcagatc
     1021 gctttgcgaa acgggtttat cagccagcag agccgcacgc acggcctcga ggttaaaaga
     1081 tttatatcca agcttaatgt cgtaatccat caactgtttc gcgccaggaa tataataata
     1141 ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat cctttttcgc ggagaaagcc
     1201 taaaacctta ttattttcac tgacagcaga tgcctgaaca tcagcctgtt tactgcgttt
     1261 attatcgtgg taccacacat aaccagataa ccctaaatag caaatcgcga cgccagccag
     1321 gccgatcgcg gtaatacgtg agaccatttt cattattctt ccctcacggt ggtgagttat
     1381 tgtgacccag aaaattttcc agaatgctgc aaacgtctct gcgagacaaa gccatcataa
     1441 aaaacaggga aatatgcttt ttgataggaa tcaatcaatt acatgaatat aaactatcaa
     1501 taagatagcc tgtgcctatt acccagatca ggaatggcat atcatgattg cggcgagagc
     1561 aggattgaat gttctgaaaa tgaaaaacaa cacgaaatct gtatttttat aaattccatg
     1621 gatattagtg cgttatattt tttgacaggt aaataaacgt gatccaccgc acgctttgtc
     1681 gcccaccagg cggagcgaat gactaccctt aaagaaaagc ccgataatta gcaacgaatt
     1741 tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca gacgaactga
     1801 tgatcgaggt cgatctctgc tatgaactgg acccgtatga attaaaactg gatgagatga
     1861 tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat gcgctgacgc
     1921 ctgctgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt ttccccgaca
     1981 gtaaaacctt tcccgactgc gcacctaaaa tggacccgct ggatatttta atccgctacc
     2041 gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaac cacttctggc
     2101 taccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg aaagagcata
     2161 tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg tggtcttctc
     2221 tgctggcgct gccgcagtca tatattgtcc cgggcggccg ttttagcgaa acctactatt
     2281 gggattccta tttcaccatg ctggggctgg cggaaagtgg tcgggaagat ttgctgaaat
     2341 gcatggccga taacttcgcc tggatgatcg aaaactacgg tcacatcccc aacggcaacc
     2401 gcacctatta tttgagccgc tcgcaaccac cggtttttgc gctgatggtg gagttgtttg
     2461 aagaggatgg tgtacgcggt gcgcgccgct atctcgacca cctgaaaatg gaatatgcct
     2521 tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat gttgtgcgga
     2581 tgccggacgg atcgctgctc aaccgttact gggacgatcg cgacacaccg cgtgacgaat
     2641 cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac gaggtgtacc
     2701 gcgatttacg cgcgggggcg gcctccggtt gggattactc ttcccgctgg ctgcgtgata
     2761 ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc catcgatctg aatgccttcc
     2821 tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag aaagagacag
     2881 aagcactgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt tacctctggg
     2941 atgatgaaaa cggtatctac cgcgattacg actggcgacg cgaacaactg gcgctgtttt
     3001 ccgctgccgc cattgtgccg ctctatgtcg gtatggcgaa ccatgaacag gccgatcgtc
     3061 tggcaaacgc cgtgcgcagt cggttactga cacctggcgg gattctggca agcgagtacg
     3121 aaaccggtga acagtgggat aaacctaacg gctgggcacc attacaatgg atggcgattc
     3181 agggatttaa aatgtacggc gatgaccttc tgggtgatga aatcgcgcgc agctggctga
     3241 agacggtgaa tcagttctat ctggaacagc acaaactgat cgagaaatac catattgccg
     3301 atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt gcaggatggg tttggctgga
     3361 ctaacggtgt ggtgcgccgt ttaattggtt tgtacggtga accataatat ttttacagcc
     3421 agccgctaac ttcctgctgg ctgtaaaatt atcctcttca ggaggagata tttaacatca
     3481 ttgccgcctg ggtgcgattt ttcacttcca gacggcgata cagggattcc agatgcgctt
     3541 ttaccgttcc ggtactgata ttcagcgccc tgccgatctc cttatttgat tcgcccgccg
     3601 ctaacatggt taaaatctcc cgctggcggg cgcttaacga tttgagatct ttaatgtcct
     3661 tttccggcgt cgtccgccag tctccaggca gaaacatcat ccccatcgcc gcactattta
     3721 ccgccaacgc aaatgtctcg acggttgaat cacgaggcac aatggccagc acattaaaat
     3781 ggataacttc ctgtaaccac cgtttattgc aatccgtcgc cgtaattaac accttaacct
     3841 caggaaattg caccacggtt ttttgcagta accagtagca aaactcacca tcctgatcgc
     3901 catcgagcat cactaaggct tcagggtaac tttccagctt ttgccataac tcatctgcct
     3961 gactggcccc ctgaatactc actcctggaa tacgctgctg taaactgatt ttcattccat
     4021 gaataaatat tgactgcctg tcaaacatga ctatttgcat aactgaatct ccacctgaat
     4081 acgttaaaaa gacttaagta gtggaagggt attacccgcg agaaaaaata agaattcgcc
     4141 atttggcggt ggccattcta cagagatgac gcgtagaaaa tagttaccga tataaatcgt
     4201 tacagctaaa cgcctgaaat tacatgtcga gggcactatt taaaacaatt ttgaggattt
     4261 ccttatattg atggttagta cgcatgcaat taaaaatgaa attccgcgac cacaagcaaa
     4321 aataacaaat ggcaatgaga caaaaataag cacaaatagc caacacgtcc tctgttcact
     4381 ttaaagggaa tcgctgaaaa aaaacgctct gtttagggaa attcaccttt ctcataaagc
     4441 tattccgccc ttttcctgct gagaaatcgc cacattcgtc atgacaacat tgtgaaaccc
     4501 ggcattagat gttagaaaaa acaataaaca atgcgatatg cgcgttactg tttcgctgtg
     4561 aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat gcagttgttc atcaaagtcg
     4621 cggagctgga aagtttttcc cgcgcggcgg atttctttgc tttgccaaag ggaagtgttt
     4681 cgcgccagat acaggcactg gaacatcaac ttggcaccca gcttctccag cgcaccacgc
     4741 ggcgggtcaa actcacgcca gaaggcatga cctattatca acgggcaaaa gatgtgttga
     4801 gtaatctcaa cgaactggac ggtctgtttc aacaggatgc caccagtatc agcggtaaat
     4861 tacgcatcga catcccgcca ggaatcgcga aaagcctgtt actgccgcgt ctgtcggagt
     4921 ttctctatct gcatccggga attgagctgg agctgagtag ccatgaccgt ccggtagata
     4981 ttcttcacga tggttttgat tgcgtgatac gcactggcgc gtcaccggaa gatggcgtta
     5041 tcgcccgtcc cctcggcaaa ctgaccgtgg tcaattgtgc cagtccacac tatctgacgc
     5101 gctttggtta tcctcaaagc cccgacgatc tgacttcgca cgcaatagtg cgttacacac
     5161 cgcacctggg tgtacatccg ttaggttttg aggttgccag cgttaatggc gtccagtggt
     5221 ttaagtctgg cggcatgttg acggtaaaca gtcgcgaaaa ctatctcgcc gccggtcttg
     5281 ccggtctggg gattattcag atcccgcgca ttgccgtgcg cgaagccctg cgtgccggga
     5341 ggcttattga agtattacct ggctaccgtg ccgagccgct ctccctttcg ctgatttatc
     5401 cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat gcagtggctg gctggcgtaa
     5461 tgaaagagca cctggactga ccgactatac tttttaagaa tgaccacgac agaaggacaa
     5521 aagagcggat gacgcaggaa aacgagatca aacgtcccac ccaggatctg gagcacgagc
     5581 cgattaagca gttggataat agcgagaaag gcggcaaagt tagccaggcg ctggaaaccg
     5641 tcaccactac tgccgagaaa gtccaacgcc aaccggttat tgcgcacctg attcgcgcaa
     5701 cagagcgctt taacgatcgg ctgggtaacc agtttggtgc ggctatcacc tatttctcat
     5761 ttttgtcgat gatcccgatt ttgatggtgt cgtttgccgc agggggcttt gtgctggcct
     5821 cccatccgat gttgctacag gatatcttcg acaaaattct gcaaaacatc agcgatccga
     5881 cgctagccgc cacgttgaaa aacaccatca acaccgccgt tcagcagcgt actaccgtag
     5941 ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa ctggatgggt aacctgcgtg
     6001 aagcgattcg cgcccagtcg cgcgatgtct gggaacgctc gccgcaagat caggagaagt
     6061 tctgggtaaa atatctgcgt gattttattt cgctgattgg tttgttgatt gcactgattg
     6121 tgacgctttc gatcacctcg gttgccggtt cggcgcagca aatgattatt agcgccctgc
     6181 acctgaacag cattgagtgg ctgaaaccga cgtggcggtt gattggcctg gcgatttcca
     6241 tcttcgccaa ctatctgctt ttcttttgga tcttctggcg gttgccgcgc caccgtccgc
     6301 gcaaaaaagc cctgattcgt ggaacatttc tggctgcgat tggttttgaa gtgattaaaa
     6361 tcgtgatgac ctacaccctg ccatcgctga tgaaatcccc ctctggcgca gcatttggtt
     6421 ccgtgctggg gctgatggcg tttttctact tcttcgcccg tttgacgctg ttttgcgcgg
     6481 cgtggattgc caccgccgaa tataaagacg acccgcgaat gccggggaaa acgcagcggt
     6541 aattcattgg tttataatgc ctgataaacg cacggtcgat cccctcgccc cttcggggag
     6601 aggattaggg tgagggggta caagccagcc agagaccagg caacgacatg cacataacca
     6661 cctgaaacta catctcttca ggagcaaaca atttcatacc aactcataac cccagcatat
     6721 aaatccagtt ggtaactttt atttaacctg aaaccagttt tatccactat ttataaaatt
     6781 atgtgaagca tttcatagaa gaaaaatcac tggcctaaac attatcccct ttttgcctgg
     6841 tttttgacca tttccgcgat ttgttacaca ttgaaatatc acttttgctg tgcgtaatat
     6901 ggctattcgt tagccaaaaa ataagaaaag attatgcaag caacagccac aacactcgac
     6961 cacgagcaag aatacacgcc gatcaactcg cgtaataaag tccttgtcgc ctctctcatt
     7021 ggcacggcca ttgagttttt cgacttttac atttacgcca ctgcggccgt tattgtgttt
     7081 ccgcatatct tcttcccgca gggcgatcct acggcagcaa cgctccagtc gctcgccacc
     7141 ttcgccatcg ccttcgtcgc gcgccccatt ggctctgccg tttttggtca ttttggcgat
     7201 cgcgttgggc gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg
     7261 gtgattggtc tgctgccggg ctatgccacg attggtattt tcgccccgct gctgctggcg
     7321 ctggctcgat ttggtcaggg tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg
     7381 gcgactgaaa acgccccacc gcgcaaacgt gcactgtatg gctccttccc gcagctgggc
     7441 gcaccgattg gcttcttctt tgccaacggc actttcttgc tgctttcctg gctactgacc
     7501 gacgagcagt ttatgagctg gggctggcgt gtgccgttta tcttctcggc ggtgctggtc
     7561 attatcggcc tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct
     7621 aaagcgaaaa agcaggtgaa gatcccgctg ggtacgctgc tgaccaaaca cgtacgcgtc
     7681 acggtgctcg gtaccttcat catgctggca acctatacgc tgttttacat catgacggtc
     7741 tactctatga cctttagtac cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa
     7801 gtgttgtgga tgctgatgat ggcagtgatt ggttttggcg tgatggtacc agtcgccgga
     7861 ttactggctg atgcctttgg tcgccgtaaa agcatggtaa tcatcaccac gctgatcatc
     7921 ctgttcgcgc tgttcgcctt taacccgctg ctcggttctg gcaacccgat tctggttttt
     7981 gccttcctgc tgctggggtt aagtctgatg gggctgacct tcgggccgat gggcgcgctg
     8041 ttaccagagc tgttcccgac agaagtgcgt tacaccggag catcattctc ttacaacgta
     8101 gcgtcgattc tcggggcttc cgttgcgccg tatatcgcag cctggttgca gactaactac
     8161 gggttaggtg cggtggggtt atatctggcg gcgatggctg gcttgacctt aatcgcccta
     8221 ctgctgaccc atgagacgcg acatcaatcg ttgtaataag agtgaaaagc ctgatgcgct
     8281 acgcttatca ggcctgaatt tccatacaat atattgaatt cgcaaagatt tgtaggacgg
     8341 ataaggcgtt cacgccgcat ccggcatgaa caaagcacac tttgccaaca ctctaaatct
     8401 gtttaattgc cggatgtcag acatccggca attatcatca cttcttcatc tgcgacaaaa
     8461 ttgtccggca ctgattagcc tccccttcgg aaggtgagat cagcgccagt aacgccgccg
     8521 ctggcgtaac cagcgttgcc agtgccgccg caacagcacc acgggcaatc aacggcccgg
     8581 ctttcacccc agcctgcgga tttttaaatg tcccacgcac atacagcggc gaacgcagcg
     8641 tgataatccg aatcccttta ctttctggat cgatagtcaa atccagttgc tccgaagcaa
     8701 aacttgccgt gccggtaaca ttgatcaacg cattctcagt atcaaaagca aaaatctgcg
     8761 gacgcgccac gccgttggca atatccagat tcgccgccgc acagttcacc cgcacctcat
     8821 cgtcaccaaa tatcgcaccg acaatgtagt taccgacatt cagcccgaca atctccatca
     8881 ggttgcggct caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta
     8941 aaagcgccgc caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca
     9001 gcgtcttctg catcagttct acatcgggca tcagttcttt cagtttcagc cgacgagcct
     9061 gaatatctgc ccgcccttgc atcggttttt tatcaccttc cagatgaata ttcgccgcaa
     9121 tgctgccgcc cgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga
     9181 tgatatgagt agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt
     9241 tgaagcgaac atcggcatcc atgacgtccc atttatcggt ttcgaagcgg tcataaggca
     9301 gcactttgcc cgcaggctga acgctttttt cacccttctt ctgttcagac cgtttcgact
     9361 tttcagctcc tttcccggaa tcaacgccaa tcaacggtcc caagtccgcc agccgcaatt
     9421 gccgcgactc gacatcacct tccagttttg gtcgtggctt gccggtggtg tagaccagag
     9481 aaccgtggat atcgctatcg ccaatgcgcc cattaaaacc gcgataatca aagaccgacg
     9541 atttttcagt gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg
     9601 gcagcagaac gcccgtcagt tcatagagat cacccagcga atcgccagaa aatttaagcc
     9661 gtaaatcgac accacccatc ttcattgggt cattcacaac gccatcaaaa gcaacacggg
     9721 tgttaccaga gcgaaaatca gcctgtaccg gaaatggtgt accttcgcca cgcagcgcca
     9781 gcataccgcc tatttttccc gtaccagtga gcggttcacc gttataacgt ccctgcgcct
     9841 tcaggccaaa aacgtaatca cccacctttt ctttatccgc tttacctttc gatccagtaa
     9901 cttcgctgaa cggcagcggc ttgcctaacg gatcgacaaa aatttccaga tccgctttgc
     9961 ttactttgtc atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg
    10021 accatgccga cggctttgca ttcgcgtctt tgttgccatc gttggcaaga ttaaacgtcc
    10081 agttattgtt cttttcagag aggcgaatca ggcgtgcgtc gggcttttcc agtttgatcc
    10141 acggtagcca gaccgttttg gtcagcagcg ccagcggggc cagcgttgcc tctacgcgtg
    10201 gcaaatgcac cattgtgact tcgggaatat ctggtgggtt gccgagaatg atgtcttccg
    10261 cgtgtatatg gggccacggc acccagctgc gccagccagt ttcttgtttt tgtcgctccc
    10321 acaccacgcc cagatcgcca cggatagcga acggacgatt caactccgca gagactttct
    10381 ggttgatggt cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga
    10441 caatcaacaa caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg
    10501 ttcgcttcct catattcttc ctgtgccagt cctaaagata gtccagccag tcggaaaatg
    10561 aggcagctta tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa
    10621 gaaaccagcc ttgtgcggcg aatgcgggcg agttctgtac atcgcgccac tcttccggcg
    10681 tttctacgcc ctcgacgatc accccgcgac aatagcgatt cattagatgt aaaagctgag
    10741 aaaagagtgt gcggccttcc ggcgactgac gcagcatcac aaacagttct cgcgcgattt
    10801 tgatgtagtc ataacgcact tcacttagtg cagagaaatt tgccatcccg gtgccaaaat
    10861 catccagcca cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac
    10921 ggatatgctc caccagttcg aaacgcagcc agggaagacg ctcaatctgg cgcaggattt
    10981 ttggttgctg acgcagggcg atgagcgtag gtccatcaat attgaccgat gccagcaggc
    11041 cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct
    11101 ccatccgatg gctgacggtg atttcagtaa aatagcgatc cggcgacagg cgttgcgaag
    11161 ggttcaaggg atgcgtgacc accgttaata gctccacggc cattaaccgc ccgcatgttt
    11221 gatagatcgg ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca
    11281 agctctcgat gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag
    11341 atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgcaaggg
    11401 cagaacttta ccgcctgacc ggagtaaatt tccccactcc acaacggaat ctttcaggaa
    11461 tacgtgctgg ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac
    11521 cacagcgcaa actaacgtta attttttaca gatcaggttc acgactatgt ccaaaaagat
    11581 tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg
    11641 tttcggcggc gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc
    11701 attaaccgtt cattacgtaa cggcgctggg aacggacagt tttagccagc agatgctgga
    11761 cgcctggcac ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc
    11821 gggcctttac tacattgaaa ccgacagcac cggcgaacgt acgttctact actggcgtaa
    11881 cgaagccgcc gccaaattct ggctggagag cgagcaatct gcggcgattt gcgaagagct
    11941 ggcgaatttc gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag
    12001 ccgcgagaaa ctgctttctt tgttgcgcga atgccgcgcc aacggcggaa aagtgatttt
    12061 cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca
    12121 gcaaatgctg gaatgcacgg atatcgcctt cctgacgctg gacgacgaag acgcgctgtg
    12181 gggtcaacag ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt
    12241 ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga
    12301 tgttccggcg gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc
    12361 tttcagtgcc ggttatctgg cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa
    12421 acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga
    12481 ggcgatgccg gcgtaaaagc agcatatgca aaaggctgga taaagtgtta cccctctatc
    12541 cagctcatcc ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc
    12601 gggtggaata tccgtcactt caggatgaac gtcatccgtg gcgacagagg tggtcgcagc
    12661 ggcagtagat ggggtcatga tttgatccca ggccgcctgc aacgctttca tgttaaattc
    12721 cggctcgcct ttcggctgca gcagtattaa cgccatatcg ttcgacaact gctgacgcag
    12781 atcctgattt aacatctcca ccgtcaggct gttaaggaag tcctggcgca gtttctgata
    12841 ttgctccggc gcgatatcga caacctgatt ttgcaacgaa cgcatccgct gccccatcag
    12901 aatatcggta tcagctcgcg cataggcggc aaacagtttc tgcagctcca gtttcttttg
    12961 cgccactaac gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc
    13021 acgcgccacc agattaaggt tgctgctcag cttgtcgttt ggtgattcga tgttaatggc
    13081 acactgcgca cgcagataca gcacacgaca gtcaaatcca agaccgatat ctttgctgtt
    13141 actggcgctt aacgcttgct gaacatgcca gaacagcgcc tcacgggcca ggtccgcacg
    13201 ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat
    13261 tgataaccgg tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg
    13321 agaaagcgtc ggcaccggag ccggtgtttc acgtttgcct ttcagttcgc caaacgtttt
    13381 attgatttgg tcgacgaccg agcgcgcatc cacgtttccc accaccagca gcgtcattgc
    13441 atccggggta taccatttct gatagaaatc tttaatcttt tccgcttcaa cgggttgttt
    13501 cagcggatcg gcaggatcgt gacctaacaa ggttgatcct ttcagacgat agcgccacca
    13561 gccctcttta gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggtt
    13621 gatggtttct ggcgtgatag tcagtttgcc agtggcattt gccagataag agagcgcttc
    13681 tttcagcaag tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga
    13741 gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc
    13801 ctgtgctgct tcaaggccac cgctttgcgt taacgcaata cgagggatgg cgtggctgta
    13861 accgctctgt tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac
    13921 acgatcgctg ggacgctggg gcgtggtcag cacttgccac tgtaaaccgt tggaaagcgt
    13981 cccctgttgc catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc
    14041 catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga
    14101 tcaactatcc tggtaataaa aaactgcccg ttgtcggtca gtctaaataa cgttaaaaac
    14161 acttcgtgtt agaccgcaag aacatgaaaa tgtcacggaa gaagtgaaat aaacctgaac
    14221 tcacccaggt ggtaagttca ggcacagggg tcaattatgc gcaaacaccc gcactcgggg
    14281 aagggagtgc gggcataagt ggtgagatta agaggataat tcgtgcgttt tgccatccgg
    14341 cgcacgatta ttcagcacat cgtccagttt tttgtggtcc agttctttca cccacttagc
    14401 aacgacaatg gtcgctacgc cgttaccgac caggttagtc agcgcacgag cttctgacat
    14461 aaagcggtca ataccgagga tcagcgccag acccgctacc ggcaaatggc ccaccgcaga
    14521 gagcgttgcc gccagcacga taaaaccact acccgttacc cctgccgccc ctttagaaga
    14581 aagcagcaac acgattaaca gcgtgatttg gtggacgata tccatctgac tgttagtggc
    14641 ctgggcgata aacaccgccg ccattgtcag gtatatcgat gtgccatcaa ggttaaacga
    14701 gtagcctgtc gggatgacca gccccaccac cgatttgcgg cagccgagtt tctccatctt
    14761 gtcgagcata cgtggcagcg ccgactcgga agatgaagtc cccagtacaa tcagcagttc
    14821 ttcacggatg tagcggataa atttgaagat actgaaaccc gtcgctttag cgattgaacc
    14881 cagtaccagc accacaaaca ggatacaggt gatgtagaag cagataatca gctgccccag
    14941 ttgcaccagt gtgccgacgc cgtatttacc gatggtaaac gccattgccc cgaacgcacc
    15001 aataggtgcc agacgcatga tcatattgat gatgccgaag atgacctgcg agaaactttc
    15061 gatcacgtta aaaatcagtt ggcctttgct gcccagacgg tggagcgcaa aaccaaacag
    15121 tacggcaaac agcagcacct gcagaatgtt accgctggca aatgcgccaa tgacgctcgc
    15181 cgggatgaca tccataatga aggcgacaat gccctggtct ttcgcctgat cggcgtaaac
    15241 cgctaccgct ttcgcatcaa gcgttgccgg atcgacgttc attccggcac caggctgcac
    15301 gacgttaacg atgataagac caataatcag cgcgatggta ctgacaattt caaagtaaag
    15361 cagtgcgact gcgccggtac gaccgaccgc cttcatgctt tccatgcccg caatgcccgt
    15421 tacgacggta caaaagatga caggagcgat gatcatctta atgagcttaa cgaacccgtc
    15481 gccaagcggt ttcatttgct cgcctatttc agggtagaaa tggccaagga gaataccaat
    15541 ggctatcgct gtcaggacct gaaagtaaag gcttttaaac agagaggttt tcatagggtg
    15601 tcctttagta aaaccacagg tcttgtaagg ttatggggta cctgcggcct taaaataaca
    15661 cccagacaac atcacagaaa tgtacctgga tcataattga aacaaaaagg ttaaaaagtt
    15721 tgagctggct cgcacaaacc agcacttttt aaagttttgt aatcagtttg gggtagctac
    15781 ttttcttcca ggtaactctc ttcgaagatt tcaataggga gtgggcgagc aaaaaggaag
    15841 ccctgggcaa taccaacgcc cgcttttgcc agccagtcgc gttgtgcttc agtctccacg
    15901 ccttcggcaa tcatttgtaa gttcaggctt tgcgccaaca tgataattgc agcaatcatg
    15961 ctgctatctt ccggcaagcc ttcaacaaac attttgtcga tttttaacac gtcgattggc
    16021 aacgatttca tatgctgcag ctgacgcagc cctgcgtagc ccatgccgaa atcatccagc
    16081 gccacccgaa ctccggcatt gcgcagcgga cggaggattg ccaccgcagc atgagggtcg
    16141 tcaatacgtc ggctttctgt cacttccaga atcagtgttc ccggctgaat gcgatagcgg
    16201 gttaacagtt ccagcatatc cgccaccata ttcgggtgca tcagttgcag cgcagagagg
    16261 tttaccgaca agggcagcat aatgccgcgc tcttgccagg ctgcaagcaa tcgacaggac
    16321 tcttccagca cccagtgacc gacggtaacc atcagcccac agcactcaat gcgatcgatt
    16381 aagccatccg gcagatccca actgccatcc ggttgctgga tacgcagtaa cacttccgca
    16441 ctgaccagtt taccgctgtt catctcgacc tgtggctgta accaaatcgc aaactgatga
    16501 ttttccagcg cattaaggat atcgctctct tccgtcaacc gctgctgggc ggcttccatc
    16561 tgctgcggat caaagaactg aatctgattc ttgcctttat ggcgagcggt aaatgccgca
    16621 gaaatagcgc gactgtaaag ctgttcggcg gtgagatcgc cgtagaacat cgccacgcca
    16681 atgctacagt gcggacggag ttgaatacgt tcaatcggca ggcgctcgct catgatagtg
    16741 agcacttgct gacctaaagt gattgcgtgc cacggttcct gtacaccatt ggcaatgaca
    16801 gcaaagtcat aaccactaat ctgcgcgagg atcatacgtg gcgacagtac cgatttgagt
    16861 ttttccacca gcgtcagcag cagaatttct cgttgcgcct ctttcagcac gcccgcagta
    16921 tcacgcaggg tttcacaggt gatgatcatc agcgcggtgg tttgtttacg cgcgacaacc
    16981 tgctccagca tctccatcag caaggctttg ttcggcaaat ccgacaccgg gaagcgcatc
    17041 gcgttttcgt tctgttcttc ataatggcgc tgcagcaatt gctggttgag gttgtaactg
    17101 cgcaccaaca taccgatttc atcatcctga tgcagacgcg gtaatgccag ttggtgacca
    17161 acaagctcct gggctgggat ggcgttaagt tcgcgagcaa tattgcgtaa aggatgtaaa
    17221 atcagtcgat taatacacca gctgatggcg acggtcagga taagcgacaa aagtaagtaa
    17281 atggtcacta acgttgagag ggtgctcatc acgaacttat acatgcggaa ggaatccgcc
    17341 tgtagcacca gataggcaat tggctgcggg tttgccggac gttccagcga gtaaacgccc
    17401 agcgagattt gcaccggtag ctcaaacagg cgagtaacca ttaccggcac cggacgctct
    17461 ggaataaaac ttttacgcag tgcctggaac tggttgggca gcaccacatc ggcacggctg
    17521 accacgcctg ccggtttaat gctggcaaga atagcttccg cttcgggaat atcgcctttc
    17581 aaaatagctg aagataaggg ttcgcggaca gagcgagcaa tgctttccag ttgcgtagcc
    17641 gtgttatagc gattctgctg gaccagatgg aacagcaaaa cggtgcaaaa aataaaaacg
    17701 aacaccagga caacggctgc caccattgcc atctgcttga ttgttaacga gcgacttacg
    17761 cgcaaattgt ctccccccgg aaatcccaga ttgccgcccg agtatacccg atcgcagcgg
    17821 cattaagaga ggcgctatct gaaaacttac cagtcggcgt aaggtatcag cggctgcggc
    17881 ggtaaatcca tgtcaccctg ccatccggcg gcggaataac gtacgtagag caggaaatgg
    17941 ctgggtgcgt aatctttcgc ctgctggata tcaattgccg tgccgacaaa ccagttggaa
    18001 gtaacacgtc gttcaagtaa tgcccgcgcc gtgtagccaa agccctgact gctgccgcca
    18061 tcgttcgatt gccgcgcagc ttcttcctgc cagtcggtcg ggatcagatt catcagcgga
    18121 taacgcggca tggttttggt gcgtgaatgc gaccacgagc cagacgcccc cagctcccac
    18181 gaccagtttt ccgtgcgctc ccgccacatc accggtatgg caaacgacag gtattcctgc
    18241 gggctgtagt aaccgccctg accgagtgag tagccactca ggtctttgtc gtaatgccag
    18301 atcatattgt taagaccaat ggtcacacgg cgattattct ggttgatgat cttatagtaa
    18361 tagcccgtca tccagcgcac gcgccagtta tcttcgacat ttttaccggt taactggtca
    18421 ccgctaagcg atgcccagac gccgtttgct tcacctttat cgtagctcag gcttagcccc
    18481 acgccgtcgg cacgcacgcc gccccatttt ttcccggtat tgctcgggga gtctttttgc
    18541 ccgccaaagg ccagcaaaga actggagatg ggccgacgat gggcgttaac ggtgtaaccc
    18601 agcggcccga tatcatcgct gtaactgatg ccgccgacca catccaccac attgaagccc
    18661 atcggcgtgg tgccgatatc ccagctccag acgtcatttc gccagccgac cgccacgctg
    18721 gcaccggaat ccgactggct gcggttgccg ctacagtctt gtaatgtaca ggtgccccag
    18781 ttgtcatccc atttgccatc ggcattagtg gagaaactgc cgacgttcat attgacgaaa
    18841 tcactgcgaa agaacatccg cccgtcagaa tacggcgcat ccacctgcaa catggtagtg
    18901 tgcgctttca gatcggagta accaccggtg ccgctcgatc cccaataatc gtgctcaagg
    18961 gtgacgttaa gatcctgctg gcgatagagg tccgctgcat cgctgcgcac gccgcgtttc
    19021 agccagtcat ctttctcgtc gttacgggtc agccgggtaa aggtgtcgtt atcctgcgga
    19081 cgcgtcgtgg tcacaccgga tgcgaccata gcgtctttgt aggtttccag cgcctgcttt
    19141 ggatcgcccg cccgcgcttc aaacttcgca ccgtcgcgca gcaccatcgc actttccatc
    19201 gacggcggct gagattttgc cagcgggatc aactgattaa acgtctgctg cgccgctgcg
    19261 gtatcgccaa gctgcgcctg tgccagcgcc acgcgccgct gtgtgttcag cgaggcgtta
    19321 tcggtagcgg gcagtttcgc cagctggcta cgtgccgccg ctttgtcacc ggcagcaata
    19381 tccacttccg tcagaccaag aatggcatcg gcgttagttg gctcccgcgt caggacattc
    19441 tgatatgcag cgcgagcggc ggtgtaatca cgtcgttgtt gcgcccagtc agccagcgtg
    19501 aggtctatgc gcgtggaagg tggttgctgg cgcagcatcg cttccgcttc agcttctttg
    19561 ccgctttctc gcaggcgatt agcggtttcc agtacctgat cgctttgcag tcgattaacc
    19621 agctcctgaa tattgctgct ccactgcgcg cgcggcaggc tgttgatatg cgccagcgcc
    19681 gctctgtcct ggtcatgacc agagagatac agcccgtaag cgtaaacctg ctccgggtcg
    19741 tttggcttct gctgtgccag attgcgcatt aatgtatcag cctggctgcg ttgtccggcc
    19801 tgccagagat cctgcgaaag tcggtaagta atccatacgc tgccgggatc cagcgccagt
    19861 cgttgccact gaagtgctgc cgcctgcgcc catttgccct ggttttccag tgcctctgcc
    19921 tgctgtgcca gacggtcgtt ttgcaggctg cgttcgatat catcaatgct tcgccgctga
    19981 ctggcagaga gcgaggcgat aaacgcttca gctttttctg gcgattgctg gcggtatata
    20041 ttcgccagcc cgcgcacggc gttagtgttg ccgctgtcca tacgcaaggt ctgctgataa
    20101 taacgttcgg cggcgggata atcttttcgc gccatcgcca catcgcccag ccccagcact
    20161 gcatagctgt cggtgttatc gacattacgc gcctgctgga acaggcgttc tgcccggtca
    20221 ggattattgg ctttcagcgc agcatcgccc tgctggatcg ccagccagta gcggtttact
    20281 ttcaggagac tgttccattt gtcgttgttg ctgctgtgcg gatccagggc gagggctttt
    20341 tccagattcg ccaccgcatt ggcgcgatcg cctttctgag aatacgcctg gcccagcgcc
    20401 cccagagcct cactgtcttt cgggttcgcc cgcactgcct gttgtagttc gggaatggct
    20461 ttacccgcca taccagagtc caccgccgct aaaccttgcg cacgagcgcg gaaagcaggg
    20521 tcggccagct gtttttgctg ttctgccagt tgcgattgcg ccgcagctac gctatcgcca
    20581 tcactaaaga tcgagagata ttttttcagc gccgacacac tggcatcgct gacgggcatg
    20641 tctttaatct gcccgtacca gattttagag gccccttcgc gcccggcgtt tgatttcgcc
    20701 atctgttcca ggacggcaaa accttcgtca cggcgatcgc tactaaacag caatagcgcc
    20761 agattgtttt gcaggcccgt attgcccggc gtatccgcat taatgcgttt tagctgatta
    20821 atcgcttcgc cacggcgagc cggaattttc gccaccgtac tccagtactc gacggcaatg
    20881 tcaccttccg gcggcgcacc gttgaacagt ttgttgtaac tcgccacagc ttcttctgca
    20941 tgaccagtcg tcgcctgcaa tcttgcctgt tgcagtgcct gacgaccatc cggcgtggaa
    21001 agcaacattg tggtccgcga cgatttatac gcatttgaac tcggcgctaa ctgcgacagc
    21061 cgatcgagct gtttttgcgc gccatcaata tcgccctgac gtaacagaga acggaaacgg
    21121 gcggcaacga cgtccgggtt attcggatca ataagttcca gccgatacaa cgactgttgc
    21181 accaggtctt cacgatgggt cgcttcgcct aaccgaactt gctccagcaa ctgttgctga
    21241 gcggttggtg ctgcctcgac catcggcatg acggccagac cgagggaaag cgtgaatatg
    21301 tttagtgtga atttgcgcat tcctggcccc agtcaggtaa taactcacct tttgtcgaga
    21361 agcggaaacg gtgttgatcc cagccttgtc caaacagggt cagcacatag ttgtaatagg
    21421 catcgctgcc gggaaagtta tcggccacgc gctggcgctg aacggcctgc gcatcgcggt
    21481 tttgtaaaaa gggcagcatg gcggcagaaa aaccgaccgg tcctttaccc tgcgctttcc
    21541 ccgtagccac atccactttt tctggcggat agccgttttt ctcggtgaat gtcgccatcg
    21601 gtttaaaccg gttgagcatc cgcgcttttt gcggatcgct gtcaggcatc atgcctaccc
    21661 acatgtaaac gcggatcgcg tcgtagctgc tgatcaatgt tttttcggct tttagctgcc
    21721 agcctttgtc tttctcatag cgcacccagt ctggcgaaaa gcctttcggg gcggtttcca
    21781 gcaataaacg ttgattggtt tcgcgcagcg tagtccacgg cgcgccaaag cgggtgaaat
    21841 actgcgccag cgtcggcggc aggtagctgg ggttaaaacg ccagctgtta tcctctgcaa
    21901 atcccacttt gcccggcaac aacatggagc ccagcccagg caccgtcacc acttcctccc
    21961 gcgcaatacg ttttaacaac gcgctgccga tgtcggtata acgctgctct ttccacaaac
    22021 gccccgcctc cagcaacgac caggccatcc agacatcacc atcggaggcc gaattgctgt
    22081 ccagcacttc ccacttactg ttctctttct tgccccacag ccatgcgggc aaacgttctt
    22141 ttaaagaacc ctgagcgaga ttgttctgcg tccagtcgag aatattatcg aaagctgcac
    22201 ggtcgttagc cgccagggca aagaacatgc cgtaactttg cccttcggaa gtggtgattt
    22261 tgcgcgcgtc gctggggtcg atgacgcgcc cttcctgact gatgtaatcc tttttaaact
    22321 gttcccaggc aggccaggta caggctgcct gaacactaaa ggcagccagc agcagcatcg
    22381 tcacgagtcc actacgcaac acattcatct tcaattactc gttatccggg ttaagacgac
    22441 gacggctgat aatacgcagc aaacgccaca gtacccaggc cagcagcacg acgctgattg
    22501 ccgccagcac cgccagcaga atcggatggt ttgccagggc ataccataag cgttcgaacc
    22561 acggcagatg gccaacgtaa tagacgtcgc caacacgcag gctattgata ccggactcgc
    22621 ggatcaccgc gaccgaaccg aacatggtgg cgcgtttgcc gctatcgttc accgcatcgt
    22681 taagcatttc ataaccgcgt gggctatctg ccagcagcgc aatcacgcta cgctggtcgt
    22741 tatacggcga ctggaagcca atcaccgccg ccatcgcacc ggaagaggtc agcgttgact
    22801 gggtttctgc cgcgcgatcg ctctcgtccg gcacaatgcc ggggaacggg gtctggcgca
    22861 tcggcgtttt cacccagctt tcggtcgcct gcaccagcag gtcgatctgc ttgtcgtctt
    22921 ttaatttgtc cgggatacca ccgatgatca tgatgtcggc atctttgccc tgaatggtgc
    22981 tgccatcatc ggtcaccgtc agattaatcg ccgggaagcc cgtctgtgcg ccgataaaac
    23041 caacagtatt cagcaaggtt tccatctgtg cttcgttagg cgttttcggc atcacggtga
    23101 tggtttgtga cagatccgcc atccggctga acgggaagcc cgcgttagca aaggcgcgta
    23161 gatccggcat cgggatgaag tggtaatact tcgagaagtc gatggtggaa tcatcaccaa
    23221 tcaccacatg attctgcacc ggctggaagg taatgcagtt atccaccgaa ccgcccggca
    23281 tcgggttcat atactcaaag tcaaagcgca gctggttggt cgcgcccagt ttcagcgccg
    23341 gaatagagac atccgttttg ccatccagca aaccttgtaa taccggaatc cgcagcagca
    23401 ggcgattcgc ctcctgtttg ctgctcaggt tgaaggattg caggaactgg ttattcaggc
    23461 tgatatccat ccgcgaactg tctttcaccg gcggcatggt gtagcgataa ttaatatcca
    23521 tatcaatgcc ggtactgcgc atcaggtaga gatccggcgg caggtttagc gaaacgttaa
    23581 tcgctgctgg ctcaagaccg ctggattgta actgttcttc ataggttttc agttcaccaa
    23641 aggtaaccgg acgatcggta cgcacccagt tcggcgcatc gtacggctta cgcggtagca
    23701 gcggtttcac ttcattcact accacgcttt caccacggaa cagaatgtta ccctgagcga
    23761 tacctttcgc tgcctgcaac aggtctttgt cgtcacgacc aaacaccacc agcagtttga
    23821 cataaggatt ctgcggatgg ttgatcatct caatcaccgg ggcttttacc gccggatgat
    23881 cgcgcaggaa gtccgggcgt ttgtcattgg tggcaaaaac gatggcattg cgatccggca
    23941 attggttata gagcaccggg aagttctgcc cacgccagcc agaacgcgaa ccaaaccacg
    24001 aggcgacaat ggcagatgct tgttgcagcc caacatccgg cgcacccgca aagaccatcg
    24061 gcaaggtgtt agtacggtta tcacgcgggt caaagaatgg taccgggaag tgtgacaggt
    24121 cattcttcac attcagggtc tgataggtca aatccagtcc actgctccgc ccaacatcca
    24181 gccaaagcgt ggtgctggcc gggttttcgc acacgtcctg ataatgaccg acaaactcca
    24241 gccgcacacg gttgaagtcg gtaataaaca gtgggttaat gggcatttgt gccagcgttt
    24301 ttttacccaa ctgttctttg gtcactggca gcacgcccat cagttcatca ttgagataaa
    24361 cctttaactg cgactggaca ggcagtaacg atggcgatgg cgtgtattcg aggttgagca
    24421 tcgccttcgt caccacttca tcgctgcgca taccaaactc aatgctgccg ttcgggttaa
    24481 tgccacgtag caccatgctg cccggcgggg gcgcaatttg cgcaaaggtc agcttcacat
    24541 cacgcgaagg accgttctgc gccacgatgg gggcatcagc gccctgcacg cctggcatca
    24601 cttgccccac ctgcggattt tgttccgtct gggcggctac agctggctca gcattgatca
    24661 gtggttgcgt tgctggcgtc gcctgcgtca tgaaagaggg gaacgcactc atccccatag
    24721 ccactgcaca aatccagaat agttttcttt tcatcgcgtt atcatcattg ttgagccaaa
    24781 gcctgatccg atggttgtgc cgtttcgctc cgctccgggc ggcgcggaat aaacgataca
    24841 acccaggaaa ccagagaagt cagcacacgg aatatgccct tcaccgaaga aggcgcaaac
    24901 tccgccagat ggcggtagcc acggaagccg agcttcagaa tatccagcag actttccagc
    24961 ggcttatctt ccgggtagct gtcctgccag agcgcccatg tatccgcacg ggcaaacgta
    25021 cactgcacaa aatcgatatg ttgctgggtg gtgagcggca ttaatttcaa cccaacttca
    25081 ttacccatca cgcgcgccac ctgggtcggg aagacgtatt cctgctgacc gcgtttaagc
    25141 aacagattca ctttctgccc ttccagaatc tgcgcctgac cgttgatctt gatccccaaa
    25201 ccaccgtcgg agaaatcctg aacggtacac gagaagaggt gaccatcttc gcgggcaatt
    25261 gccgcgggca tcgtcatctc cacgcggtgc gatcggcgta cctgtttgct ttctaccgat
    25321 accgcaactg cgccgccaag aacaatcagg ttgtagaata cccacaccat actgacgacc
    25381 acggtgagca tctcggttgg cgggccatag aagtagcgcc agatgcctac cgcaacgccc
    25441 accaggttga gcagaacaag gaagatgtag ggccgcgaga tcacccagtc gacgtactct
    25501 tcttccacca gtccaccttt ggcggtgacg ttaaatttgc ctttgtgcgg gttaatcagc
    25561 gccaccagcg tcggtggtgc gatataccac gccagcaccg tttcgtagat ttcactccag
    25621 aaagagtggc gatatttgcc ctggatcttg gagttggtca ggctggcatg gatcatatgc
    25681 ggcagcacga acagggcaat catcaacgct ggcgcataga tgatgtaggc atgcagcagc
    25741 aggaacgcca gcggcgcagt caggaagatc agtcgtggaa tgcccgacaa gaagtggaac
    25801 atggcgttga cgtaacatag ccgctgagca aacttcagcc ctttaccggt gagcgggtta
    25861 tcgagacgaa agatttgcac catcccgcgc gcccagcgaa tacgctgacc gatatgcgcc
    25921 gacagacttt cggtcgccag ccccgccgcc tgcggaatac gcatatatgc agaggtgtag
    25981 ccacgacggt gcaggcgcaa agaggtatgc gcatcttcag tcacagtttc gacagcaatg
    26041 ccgccaattt catccagcgg cttacggcga atcaccgcac aggaaccgca gaagaaagtg
    26101 gcgtcccaca tatcgttgcc atcctgcacc agaccataga acagcgtgcc ttcgttaggt
    26161 gttttacgga aacgccccag gttgcgttca aacgggtccg gtgagaagaa gtggtgcggc
    26221 gtctgcatca tcgccagttg tttttctttc aggaaccagc ccatggtcat ttgcaggaac
    26281 gatcgcgttg gtacgtggtc gcagtcgaaa atcgacacga actcgccttt ggcatatttc
    26341 agcgcattgt tgatgttgcc cgctttcgca tgttcatgag tggtacgggc gatatatttc
    26401 acccccacgt tttgcgcaaa ctggcgaaac tcttccctgc cgccgtcatc aaggatccag
    26461 atgttcagct tgtctttcgg ccagtcgata cccagcgagg cgtaaatggt atttttcacc
    26521 acgttgagat cttcgttgta agtcgggaca aagatatcca ccgacggcca caacgacata
    26581 tctttcggca atggcaccgg ctgacgattc agcggccata ctacctggaa gtagccgagc
    26641 accagcacaa tccacgcgta cgtttcggcg aacagcagaa taagcccgca caccaggctg
    26701 accggatcgt cccagttcag cgtagaggtg tagcgccacc agatataacg gcaagaaacg
    26761 gtcagcgaca gcacaatcaa cattagcgcc gagaagcgcc ccggcatccg ccgtacgatc
    26821 agcgctaccc cccacagcag catcaggaag ataaactgcg ccagcgggtt aaacggctga
    26881 gtaacgcaga ttaacgccag aatcagcgag aaggtgacga tgataccgag gatcaaccgc
    26941 cgcgccccgg cactcaaatg gccgagctct tttttctcat caagatgctg tgttttatgg
    27001 ctaacgcgct caggcagctc gttcatccat tgatggtaac gtccacgaat attctgcaga
    27061 cctgaaaatg cccgcctgcg cggtttcggc gtttctttgc gcgatgcacc gatcagtaac
    27121 cagcatgttt gaatgagata acggaccggg tccagcggac gcggacgcga ggcgttgata
    27181 tgcggataca ggtttttatg ttctgcgcga atacgctgcc agcgcgggtg ctccagcgga
    27241 ataaaaatcc aggccaggat caaccaaaaa cagccgagcg tcgcgctgaa agccgacgcg
    27301 ccgtgacgac gataatcgcg ataacgcccg ataagccgcg cgttgaccgg cgggataagc
    27361 aaccaccggg tcaggatact catgatttac tcccgactgg cgttttcagc ccggaatagt
    27421 tcaacaggca ccagttagcc agcgtcagta tctcttcagc cgccagcgca tcactgcgat
    27481 attcaccgac tggttgctta gccgccaggc attcagccat cgcttcatca cgatgaatga
    27541 gcatcggcag taatcggcgc tggctttgca accaaagctg gtaaatatcg tcctgaactt
    27601 gactgccgat acggaagtca ttaatcaaaa tatgcgcgcc atccggcagc gcctgctgat
    27661 gcagccggat atggcagttg gcatccacat tgacgattgc cagcgagtga tcgcacaaac
    27721 tcagcagctg gtgggttatc tgcgaggaat cacgcggtaa gtcgattaaa atccactggt
    27781 aacgcccgct ggcttttagt tgctgtaagc cggagcaaat atcgctcagc cgggtttgcc
    27841 agtgctgtgg attttcttgt tcttcaatgg ataactgacc aaaaggcagc aaatcgagct
    27901 gcgaggtata gcgcaacccg gcgtcacgcc agtcctggtc atccagcata gctctggccc
    27961 agccctgacg gtgggtaaaa tcaacgttaa atgacaggcg caacaagttg tccgggcagg
    28021 catcgaccac caggacattt tctcccaaca tttgtaatga ccaggctaat gcggcggtga
    28081 tggttgttgt ccccacgcct ccccgcaccc cctgcaatcc cagtacggcc attcaccaat
    28141 cccctacttt tgttgcgcaa actctgccag caacggccag cgttttaatg ccgcggccaa
    28201 ctgttcgcgt tgggaaatat cggcataatc aatatcaggc agtgaaaatg cctgctttaa
    28261 cgccaaaata tcattctgga agatatagcc tatcgcggga tcaggcagag tatctggttc
    28321 attgttattc atttgcttga tccctatgaa ataaccgtac cgttcatgag acaattctaa
    28381 ccgtgagcat ttcgccggtt tattgtttac ggcgagacgg gaatttgctc actttttgac
    28441 tttccgttac gcaacaaaaa aagttaaaaa cgaaaagact aaaatcacac gtcttgtgac
    28501 ctgctaaatt gattcacatg ctaaatctga taagttttaa tttcaatggt aggtttattt
    28561 cttagctttc gctagtaaac tgataatcag acaaaatgat gacatgaggg acattgtgga
    28621 ccctgtattc tctatcggta tctcatcatt atgggatgag ctgcgacata tgccagcagg
    28681 cggcgtctgg tggtttaacg tcgatcgcca tgaagatgct atcagtctgg cgaatcaaac
    28741 aattgcatcc caggctgaaa ccgcacacgt cgcggtcatt agcatggaca gcgatccggc
    28801 gaaaatcttt caattagatg attctcaagg accggaaaaa ataaaattat tttcaatgct
    28861 aaatcatgaa aaaggtctat actatttggc ccgtgatttg cagtgttcta ttgatcccca
    28921 taattacctt tttattcttg tttgcgcaaa taacgcatgg caaaacattc ctgccgagcg
    28981 gcttcgctca tggttggata aaatgaataa atggagcagg ttaaaccatt gttcgctttt
    29041 ggtaattaat cccggaaata ataacgataa acaattttca ttgttgcttg aggaataccg
    29101 ttcacttttt ggtcttgcca gtttgcgttt tcagggcgac caacatttgc tggatattgc
    29161 cttctggtgc aacgaaaaag gggttagcgc ccgtcagcag cttagcgttc agcaacaaaa
    29221 tggtatctgg acattagttc aaagcgaaga ggcggagatc caaccacgca gcgacgaaaa
    29281 acgcattctg agtaatgttg ctgtactgga aggtgcgccg ccgctatcag aacactggca
    29341 actgttcaac aataacgaag tcctgttcaa tgaagcccgt accgctcagg cggcgacggt
    29401 ggtcttttct ttacaacaaa atgcgcaaat cgagccactg gcccgcagca ttcatactct
    29461 gcgtcgccag cgcggtagtg cgatgaaaat cctcgtacgg gaaaataccg ctagcctgcg
    29521 cgccaccgat gaacgtttgt tattggcctg cggtgcaaat atggttatcc catggaatgc
    29581 cccactctcc cgctgtctga cgatgatcga aagcgtgcaa gggcagaagt ttagtcgcta
    29641 tgtgccggaa ggtatcacta ccttgctgtc aatgacccag ccgctcaaac tgcgtggttt
    29701 ccagaagtgg gatgtgttct gtaatgccgt caacaacatg atgaataacc ctctattacc
    29761 tgcccacggt aaaggcgttc tggttgccct acgtccggta ccgggtatcc gcgttgagca
    29821 agccctgacg ctgtgtcgcc ctaaccgcac cggcgatatc atgactattg gcggtaatcg
    29881 gctggtgctg tttctctcat tctgtcggat taacgatctg gataccgcgt tgaatcatat
    29941 tttcccattg ccgactggcg acattttctc aaaccgtatg gtctggtttg aagatgatca
    30001 aatcagtgcc gagctggtgc agatgcgcct gcttgcccca gaacaatggg gcatgccgct
    30061 gcctttaacg caaaattcta aaccggtcat taatgccgag cacgatggtc gccactggcg
    30121 acgaatacca gaaccaatgc gactgttaga tgatgctgtg gagcgctcat catgatgacc
    30181 atcagcgata tcattgaaat tattgtcgtt tgcgcactga tatttttccc gctgggctat
    30241 ctggcgcggc actctttgcg acgcattcgc gacaccttac gtttgttctt tgctaaacct
    30301 cgttatgtta aaccggccgg gacgttacgc cgcacggaaa aagccagggc aaccaaaaaa
    30361 tgactcaatt tacgcaaaat accgccatgc cttcttccct ctggcaatac tggcgcggcc
    30421 tttccggctg gaacttctat tttctggtta agttcggcct gttgtgggcg ggatatctta
    30481 acttccatcc gctcctcaat ctggtgtttg ccgcgtttct gctgatgccc attccgcgct
    30541 acagcctgca tcgcttacgc cactggattg ccctgccgat cggctttgct ttgttctggc
    30601 atgacacctg gttgcctggc ccggaaagca taatgagcca gggttcgcag gtggcggggt
    30661 tcagtaccga ttatttaatc gaccttgtca cacgctttat taactggcag atgattgggg
    30721 ccatttttgt tttattagtg gcctggttat tcctgtcaca atggattcgc attaccgttt
    30781 ttgtggttgc cattctgcta tggctgaacg tacttaccct ggcgggacca agtttctcct
    30841 tgtggccagc cggtcaaccg acgaccactg taacaacgac gggtggcaac gcagcggcaa
    30901 ccgttgcggc gacgggtggc gcaccggtag tgggtgatat gcccgcacaa actgcaccgc
    30961 caacaacggc gaaccttaac gcctggctga ataatttcta taacgcggag gcgaaacgta
    31021 aatcgacctt cccgtcttcg ctgcccgctg atgctcaacc atttgaacta ctggtgatta
    31081 atatctgttc gctttcctgg tcggatattg aagccgccgg gttgatgtcg catccgctgt
    31141 ggtcgcattt cgatattgag ttcaagaact ttaactccgc cacctcctac agtggcccgg
    31201 cggcgatccg tttactgcgc gccagctgcg ggcagacttc gcacactaat ctgtatcaac
    31261 cggcaaataa cgactgctat ctgtttgata acctgtcgaa actgggcttt acccagcacc
    31321 tgatgatggg acataacggc cagttcggcg gttttttgaa agaagttcgc gaaaatggcg
    31381 gcatgcagac cgaattgatg gatcaaacaa atctgccggt tattttgctg ggctttgatg
    31441 gttcgccggt ttatgacgat accgccgtgc ttaaccgctg gctggacgtt accgaaaaag
    31501 ataaaaacag ccgtagtgcc acgttctaca acacgcttcc actgcatgac ggcaaccatt
    31561 atccgggcgt cagcaaaaca gcggattaca aagcgcgggc gcagaaattc tttgatgaac
    31621 tggacgcctt ctttactgaa ctggagaaat cgggtcgtaa agtgatggtg gtcgtggtgc
    31681 cggaacacgg cggcgcgctg aagggcgaca gaatgcaggt atctggccta cgtgatatcc
    31741 ctagcccgtc tatcacagac gtccccgttg gggtgaaatt cttcggcatg aaggcaccac
    31801 atcagggggc accgattgtc atcgaccaac cgagcagctt cctggctatc tccgatctgg
    31861 tggttcgcgt tcttgatggc aagattttca ccgaagacaa tgttgactgg aaaaaactca
    31921 ccagtgggtt gccacaaaca gcaccggtct cagagaactc aaatgcagta gttattcaat
    31981 accaggataa accgtacgtt cgcctgaacg gcggcgactg ggtgccttac ccgcagtaag
    32041 cacgaaaaag gccgcagagt ttcacccctg cggcctgatc cgggcgcaaa ttgccattac
    32101 ggcagcctga cgcccgcatg acacgttact tccgcttgtt cagccagttc atgatcatac
    32161 tggcaagaat gccagcaatg accggagccg ctaaatcatg ccagaaggcc atgcccagtt
    32221 ctgcgagcgt catatagccg cctgtgttgt aatgacaacg tttcgcggct attcttgagt
    32281 ggtctagagt caagattagc ccccgtggtg ttgtcaggtg catacctgca acgtgcgggg
    32341 gttttctctc tccagcaacc aatgccacca gggataaagc ccccgcaaca ttgcgcctca
    32401 ccggaacctt tccggcttgc cgctgattct acggccattt ttgttcgtcg aaatccatca
    32461 tttcttcact ctgtgctgtg cattcagcag aatattatcc gcttgcaacc gttgcatttt
    32521 tcttacgaa
//
LOCUS       DARNVM010000037        32147 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.037, whole genome shotgun sequence.
ACCESSION   DARNVM010000037 DARNVM010000000
VERSION     DARNVM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.037"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(14..1774)
                     /locus_tag="SI621_001578"
     CDS             complement(14..1774)
                     /locus_tag="SI621_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEI4064309.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1904..2596
                     /locus_tag="SI621_001579"
     CDS             1904..2596
                     /locus_tag="SI621_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEI4064310.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2795..3883
                     /gene="serC"
                     /locus_tag="SI621_001580"
     CDS             2795..3883
                     /gene="serC"
                     /locus_tag="SI621_001580"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEI4064311.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNIPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3954..5237
                     /gene="aroA"
                     /locus_tag="SI621_001581"
     CDS             3954..5237
                     /gene="aroA"
                     /locus_tag="SI621_001581"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEI4064312.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGSFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5406..6170
                     /gene="ycaL"
                     /locus_tag="SI621_001582"
     CDS             5406..6170
                     /gene="ycaL"
                     /locus_tag="SI621_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HEI4064313.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGAASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFELLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6343..7026
                     /gene="cmk"
                     /locus_tag="SI621_001583"
     CDS             6343..7026
                     /gene="cmk"
                     /locus_tag="SI621_001583"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEI4064314.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7137..8810
                     /gene="rpsA"
                     /locus_tag="SI621_001584"
     CDS             7137..8810
                     /gene="rpsA"
                     /locus_tag="SI621_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEI4064315.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            8970..9254
                     /gene="ihfB"
                     /locus_tag="SI621_001585"
     CDS             8970..9254
                     /gene="ihfB"
                     /locus_tag="SI621_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEI4064316.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9461..11725
                     /locus_tag="SI621_001586"
     CDS             9461..11725
                     /locus_tag="SI621_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HEI4064317.1"
                     /translation="MKITTVGVCIICGIFPLLVLPQLPGTLTLAFLTVFACVLAFIPV
                     KAVRYIALTLLFFLWGISAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQVTHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGSFDSQRYAIAQHQPL
                     TGRFLQASVIEPDCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPAFRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYKWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11762..13510
                     /gene="msbA"
                     /locus_tag="SI621_001587"
     CDS             11762..13510
                     /gene="msbA"
                     /locus_tag="SI621_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HEI4064318.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13507..14493
                     /gene="lpxK"
                     /locus_tag="SI621_001588"
     CDS             13507..14493
                     /gene="lpxK"
                     /locus_tag="SI621_001588"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEI4064319.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVIWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCLPFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14530..15762
                     /locus_tag="SI621_001589"
     CDS             14530..15762
                     /locus_tag="SI621_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HEI4064320.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKNAWMQEHEAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLERC
                     PQGLFTDCRTGWEIDPVA"
     gene            15814..15996
                     /gene="ycaR"
                     /locus_tag="SI621_001590"
     CDS             15814..15996
                     /gene="ycaR"
                     /locus_tag="SI621_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HEI4064321.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            15993..16739
                     /gene="kdsB"
                     /locus_tag="SI621_001591"
     CDS             15993..16739
                     /gene="kdsB"
                     /locus_tag="SI621_001591"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEI4064322.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16893..17786
                     /locus_tag="SI621_001592"
     CDS             16893..17786
                     /locus_tag="SI621_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HEI4064323.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17763..18542)
                     /gene="elyC"
                     /locus_tag="SI621_001593"
     CDS             complement(17763..18542)
                     /gene="elyC"
                     /locus_tag="SI621_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HEI4064324.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18678..19463
                     /gene="cmoM"
                     /locus_tag="SI621_001594"
     CDS             18678..19463
                     /gene="cmoM"
                     /locus_tag="SI621_001594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HEI4064325.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19460..20782
                     /gene="mukF"
                     /locus_tag="SI621_001595"
     CDS             19460..20782
                     /gene="mukF"
                     /locus_tag="SI621_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HEI4064326.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20763..21467
                     /gene="mukE"
                     /locus_tag="SI621_001596"
     CDS             20763..21467
                     /gene="mukE"
                     /locus_tag="SI621_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HEI4064327.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21467..25927
                     /gene="mukB"
                     /locus_tag="SI621_001597"
     CDS             21467..25927
                     /gene="mukB"
                     /locus_tag="SI621_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HEI4064328.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26188..28035
                     /gene="ldtD"
                     /locus_tag="SI621_001598"
     CDS             26188..28035
                     /gene="ldtD"
                     /locus_tag="SI621_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEI4064329.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28216..28764
                     /locus_tag="SI621_001599"
     CDS             28216..28764
                     /locus_tag="SI621_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HEI4064330.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28791..29438
                     /gene="gloC"
                     /locus_tag="SI621_001600"
     CDS             28791..29438
                     /gene="gloC"
                     /locus_tag="SI621_001600"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HEI4064331.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29489..30679)
                     /gene="aspC"
                     /locus_tag="SI621_001601"
     CDS             complement(29489..30679)
                     /gene="aspC"
                     /locus_tag="SI621_001601"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HEI4064332.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30864..31937)
                     /gene="ompF"
                     /locus_tag="SI621_001602"
     CDS             complement(30864..31937)
                     /gene="ompF"
                     /locus_tag="SI621_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HEI4064333.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7818 a         7869 c         8648 g         7812 t
ORIGIN      
        1 aggctatttg attttatttt gcccagaatg ctgctttggc gcgctgcagc ttttcgtagg
       61 ccttcaacaa cgactgatgt gctgcaaacg cgtgcagatc gctatctact ggttgcaagc
      121 cataaaacgc cgcttcgccg ctcattgccg cactggcggc ttccacggca tctgcgccgt
      181 acatgcgaac aaacgcattc aaatattgca gcggctggcg atcttcttcc tgcgccagta
      241 ataacaacgt ttgcaggcag cgataatagt tggcgcgttc cggactaaat actgatgagt
      301 taaactccat cgtccattcg gtccagacca gagcctgttc cagatcgcca ccagccagcg
      361 ccagcatggc ttttaattca ccgatacgca gggtgtacca accgttatcc gacccggtcg
      421 ccagacccaa cagctcacgc acgcgggtaa agtcatcaaa accttcttca tccagttgct
      481 cgatgaggtt caggtaatct tctttttccc actcgctgcc tggtaacgaa agaatcgttt
      541 cacgtaaatg gctgcccata ctgttattcg ccagccacag atcttcagcc ggataaatat
      601 cggacatgcc aggcacgata attcggcaag cataaacgcc cagatgctcg taatcggcaa
      661 tataaacttc tttatcttct ttgttgaaga tagccatcag cgtagcaaac tcttcctccg
      721 tggtgccgga gaaattccag tccacaaacg gataatcggc atcctgcttg aacaggtccc
      781 aggagattaa accgctggaa tcgataaagt gcgtttcgag gttggtatgt tcagcaactt
      841 cttcatcatc gaaggttggc ggagtaaaca catccaaatc tttcaggcca cgaccttgca
      901 gcagctcggt cacggtacgt tccagtgcta cgccaaaatc aggatgcgca ccgaaagagg
      961 caaagcaggt gccgttagca ggattaaaca gtaccacgca aatcaccgga tactggccgc
     1021 caagcgaacc atcataagca aagattggga aaccttctgc ttccagcgtt tcgatggctt
     1081 caaccaccgc tgggtaacgc gccagcacgt ctgccgggat ttccggcaag ctgatgcttt
     1141 cagcaataat gcggtttttc acgtagcgtt cgaaaacttc ggacaacccc tgaacgcgtg
     1201 cttcgttgcg ggtattgcca gcggacatac cgttagaaac gtacaggtta ccaatgatat
     1261 tcatcggaat ataaacggtc tgattgtcgg actggcgcgt aaacggcaga ccgcaaatac
     1321 cacgatcttc gttaccggat tgcaggtcaa tcagcatact gccggtcaat tcattctccg
     1381 gatcgtaaaa tgcgcgcaga cggtcatcga gcagcccttc tggcacatcg tcattttcgc
     1441 tcagtgggaa ccatttttcg ttgggataat gcacgaacgg accgttggcg atggtttcgc
     1501 ccagccagaa atcagcaaaa aagtagttgg ttgagagacg ctcgaaatat tcaccgagtg
     1561 cagaagccag cgccgctttt ttggttgcgc ctttaccgtt ggtaaaacac agtgcgcact
     1621 ctttgtcgcg aatatgtaca gaccagacgt taggcacggg attcagccag gaggcctctt
     1681 caatctgaaa gccgaggtct gaaagttttt gctggaagcg agcgatggaa tcttccagtg
     1741 cggcatcttt gccggggata aatgtttgcg tcatgaaaat cactttagtc gtacggaaag
     1801 cgcgcaataa tacgggtttt atctcaaagg cgctatcacc gccgccatgc cgatgatctg
     1861 ttgactatgc ttttagcgga taaaccacga taagagcata caaatgaagg cgttcgatct
     1921 ccaccgtatg gcatttgata aagtgccttt tgatttcctt ggcgaagtgg cactacgtag
     1981 tctgtatacc tttgtactgg tttttttgtt cctcaaaatg accggaagac gcggagtgcg
     2041 gcagatgtcg ctgtttgaag tgttaatcat tctgacgctg gggtcagcgg cgggagatgt
     2101 ggcgttttat gatgatgtgc cgatggtccc ggtacttatc gtctttatca ctctggcgtt
     2161 gttataccgc ctggtaatgt ggttgatggc acacagtgaa aaactggaag atcttctgga
     2221 aggtaagccg gttgtcatta ttgaagatgg cgagctggcc tggtcgaaac tcaataactc
     2281 caacatgacg gaatttgagt tctttatgga gctacgattg cgcggtgtag agcagttggg
     2341 gcaagtacgt ctggcgattc tcgaaaccaa cgggcaaatc agtgtttatt tctttgaaga
     2401 tgacaaggtg aaaccgggct tacttatttt acccagtgac tgtactcagc gttacaaagt
     2461 ggtgccggag tcggcggact atgcctgcat ccgttgcagt gaaatcattc atatgaacgc
     2521 gggggaaaaa caattatgtc cgcgctgtgc aaatccagaa tggacgaagg caagtcgagc
     2581 taaacgggtg acctgacagc aaaaatatcg gttttttggt aataatccga gagattctgt
     2641 tgtgtgacgc gtgccgcatt tttgccctca acgcttttac ccattgcgat gcgtgtcact
     2701 gaatgataaa accgatagcg acaggaataa catattaccc gcggtcgcaa tcgattgacc
     2761 gggggttaat agcaacgcaa cgtggtgagg ggaaatggct caaatcttca attttagttc
     2821 tggtccggca atgctaccgg cagaggtgct taaacaggct caacaggaac tgcgtgactg
     2881 gaacggtctt ggtacgtcgg tgatggaagt gagtcaccgg ggcaaagagt tcattcaggt
     2941 tgcagaggaa gccgagaagg attttcgcga tcttcttaac atcccctcca actacaaagt
     3001 attgttctgc catggcggcg gtcgcggtca gtttgctgct gttccgctga atattctcgg
     3061 tgataaaacc accgcagatt atgttgatgc cggttactgg gcggcaagtg ccattaaaga
     3121 agcgaaaaaa tattgcacgc ctaatgtctt cgacgccaaa gtgactgttg atggtctgcg
     3181 cgcggttaag ccaatgcgcg aatggcaact ctctgataat gctgcttata tgcattactg
     3241 cccgaatgaa accatcgacg gtatcgctat cgacgaaacg ccagacttcg gcaaagatgt
     3301 ggtggtcgcc gccgacttct cttcaaccat tctttcccgt ccgattgacg tcagccgtta
     3361 tggtgtaatt tacgctggcg cgcagaaaaa tatcggcccg gctggcctga caatcgtcat
     3421 cgttcgtgaa gatttgctgg gcaaagcgaa tatcgcgtgt ccgtcgattc tggattattc
     3481 cattctcaac gataacgact ccatgtttaa cacgccgccg acatttgcct ggtatctgtc
     3541 tggtctggtc tttaaatggc tgaaagcgaa cggcggtgta gctgcaatgg ataaaatcaa
     3601 tcagcaaaaa gcagaactgc tgtatggggt gattgataac agcgatttct accgcaatga
     3661 cgtggcgaaa gctaaccgtt cgcggatgaa cgtgccgttc cagttggcgg acagtgcgct
     3721 tgacaaattg ttccttgaag agtcttttgc tgctggcctt catgcgctga aaggtcaccg
     3781 tgtggtcggc ggaatgcgcg catctattta taacgccatg ccgctggaag gcgttaaagc
     3841 gctgacagac ttcatggttg agttcgaacg ccgtcacggc taatgccgaa attttgctta
     3901 atccccacag ccagcctgtg gggtttttat ttctgttgta gagagttgag ttcatggaat
     3961 ccctgacgtt acaacccatc gctcgtgtcg atggcactat taatctgccc ggttccaaga
     4021 gcgtttctaa ccgcgcttta ttgctggcgg cattagcaca cggcaaaaca gtattaacca
     4081 atctgctgga tagcgatgac gtgcgccata tgctgaatgc attagcagcg ttaggggtaa
     4141 gctatacgct ttcagctgat cgtacgcgtt gcgaaattat cggtaacggc ggtccattac
     4201 acgcagaagg tgccctggag ttgttcctcg gaaacgccgg aacggcaatg cgcccgctgg
     4261 cggcagctct ttgtctgggt agcaatgata ttgtgctgac tggtgagccg cgtatgaaag
     4321 agcgcccgat tggtcatctg gtggatgcgc tgcgcctggg cggggcgaag atcacttacc
     4381 tggaacaaga aaattatccg ccattgcgtt tacagggcag ctttactggc ggcaacgttg
     4441 acgttgatgg ctccgtttcc agccagttcc tcaccgcact attaatgact gcgcctcttg
     4501 cgccggaaga tacggttatt cgtattaaag gcgatctggt ttctaaacct tatatcgaca
     4561 tcacactcaa tctgatgaag acgtttggtg ttgaaattga aaatcagcac tatcaacaat
     4621 ttgtcgtaaa aggcgggcag tcttatcagt ctccgggtac ttatttggtc gaaggcgatg
     4681 catcttcggc ttcttacttc ctggcagcag cagcaatcaa aggcggcact gtaaaagtga
     4741 ccggcattgg acgcaacagt atgcagggtg atattcgctt tgctgatgtg ctggaaaaaa
     4801 tgggcgcgac catttgctgg ggcgatgatt atatttcctg cacgcggggt gaactgaacg
     4861 ctattgatat ggatatgaac catattcctg atgcggcgat gaccattgcc acggcggcgt
     4921 tatttgcaaa aggcaccacc acgctgcgca atatctataa ctggcgtgtt aaagagaccg
     4981 atcgcctgtt tgcgatggca acagaactgc gtaaagttgg tgcggaagta gaagaggggc
     5041 acgattacat tcgtatcact ccaccggaaa aactgaactt tgccgagatc gcaacataca
     5101 atgatcaccg gatggcgatg tgtttctcgc tggtggcgtt gtcagataca ccagtgacga
     5161 ttcttgatcc caaatgcacg gccaaaacat ttccggatta tttcgagcag ctggcgcgga
     5221 ttagccaggc agcctgaatg aacaacgtgc aataaatagc caaatctttc tttatcaaaa
     5281 cgtcggcaca ttgccggcgt tttttttcgg accttgtgag tcattttgat taatggtagc
     5341 gtcgtttgtc aatgtaagtt attgatacat aatatttata tatgattaat caacggatga
     5401 ttcacatgaa gaatactaaa ttactgctgg cgattgcggc ctctgcagca ttactgacag
     5461 ggtgtcaaaa tacccacggt attgatacca atatggctat cagctcaggt ttaaatgcct
     5521 ataaagcagc aacgttaagc gatgctgatg caaaagcgat tgccaatcag ggctgtgccg
     5581 aaatggacag cggcaatcaa gtcgcaagta aatccagcaa gtacggtaaa cgtctggcaa
     5641 aaatcgccaa agcattgggt aacaatatta atggcacgcc ggtcaactat aaggtttata
     5701 tgaccagcga cgtcaacgca tgggcgatgg cgaacggctg tgttcgtgtc tacagtggcc
     5761 tgatggacat gatgaacgac aacgaaattg aaggcgttct gggtcatgaa ctgggccacg
     5821 tcgcgttggg tcactcgctg gctgaaatga aagcttctta tgcgatcgtt gccgcacgtg
     5881 atgcgatttc cgctaccagc ggtgcggctt cccagctttc tcgctcacaa ttaggtgata
     5941 tcgcagaagg cgcgatcaat gctaaatact cccgcgataa agagtccgaa gcagatgatt
     6001 tctcctttga actgttgaag aaacgtggca tcagcaccca ggggctggtt ggcagctttg
     6061 aaaaattggc cagcctggat ggcggtcgta cccagtccat gtttgactct cacccaccat
     6121 caacagagcg tgcgcaacac atccgtgatc gtatcgcctc tggtaagtaa atcattgtca
     6181 tctttcgggc tggtcttctg ccagcccgct ataattgcgc aataaatccc catctgaata
     6241 cagacaaaac tggtttttgc acacaacgtt aacgatttgt ggcgtcggcg cgtataatgc
     6301 gcgcggttat gttaacggta cgcctgtttt aaggagataa agatgacggc aattgccccg
     6361 gttattacca ttgatggccc aagcggtgca gggaaaggca ccttgtgtaa ggctatggcg
     6421 gaagcgttgc aatggcatct gctggactcg ggtgcaattt atcgcgtact ggcactggcg
     6481 gcattacatc accatgttga tgttgcatcg gaagatgcgc tcgtgccgct ggcatcccat
     6541 ctggatgtac gttttgtgtc gaccaatggc aatctggaag tgatcctcga aggggaagat
     6601 gtcagcggcg aaattcgtac tcaggaagtg gcgaatgctg cttcacaagt cgcggcattc
     6661 ccacgcgttc gtgaagcatt attgcgtcgc caacgcgcgt ttcgcgaatt accaggcttg
     6721 attgccgatg gccgcgatat gggaacggtg gtattccctg atgcgccagt gaaaattttc
     6781 cttgacgcct cctcggaaga acgtgcgcat cgccgcatgc tacagttgca ggagaagggc
     6841 tttagtgtta actttgagcg ccttttggcc gagatcaaag aacgcgacga ccgcgatcgt
     6901 aaccgagcgg tagcgccact ggttccggca gccgatgctt tagtgttgga ttccaccacc
     6961 ttaagcattg agcaagtgat tgaaaaagcg ctacaatacg cgcgccagaa attggctctc
     7021 gcataagcga ccgaatttgc agtacccccg ttgcaatgga atgacagcgg gtatgttaaa
     7081 caaccccatc cggcatggag ccaggtggac gttaaatata aacctgaaga ttaaacatga
     7141 ctgaatcttt tgctcaactc tttgaagagt ccttaaaaga aatcgaaacc cgcccgggtt
     7201 ctatcgttcg tggcgttgtt gttgctatcg acaaagacgt agtactggtt gacgctggtc
     7261 tgaaatctga gtccgccatc ccggctgagc agttcaaaaa cgcccagggc gagctggaaa
     7321 tccaggtagg tgacgaagtt gacgttgctc tggacgcagt agaagacggc ttcggtgaaa
     7381 ctctgctgtc ccgtgagaaa gctaaacgtc acgaagcctg gatcacgctg gaaaaagctt
     7441 acgaagatgc tgaaactgtt accggtgtta tcaacggcaa agttaagggc ggcttcactg
     7501 ttgagctgaa cggtattcgt gcgttcctgc caggttctct ggtagacgtt cgtccggtgc
     7561 gtgacactct gcacctggaa ggcaaagagc ttgaattcaa agtaatcaag ctggatcaga
     7621 agcgcaacaa cgttgttgtt tctcgtcgtg ccgttatcga atccgaaaac agcgcagagc
     7681 gcgatcagct gctggaaaac ctgcaggaag gcatggaagt taaaggtatc gttaagaacc
     7741 tcactgacta cggtgcattc gttgatctgg gcggcgttga cggcctgctg cacatcactg
     7801 atatggcctg gaaacgcgtt aagcatccga gcgaaatcgt caacgtgggc gacgaaatca
     7861 ctgttaaagt gctgaagttc gaccgcgaac gtacccgtgt atccctgggc ctgaaacagc
     7921 tgggcgaaga tccgtgggta gctatcgcta aacgttatcc ggaaggtacc aaactgactg
     7981 gtcgcgtgac caacctgacc gactacggct gcttcgttga aatcgaagaa ggcgttgaag
     8041 gcctggtaca cgtttccgaa atggattgga ccaacaaaaa catccacccg tccaaagttg
     8101 ttaacgttgg cgatgtagtg gaagttatgg ttctggatat cgacgaagaa cgtcgtcgta
     8161 tctccctggg tctgaaacag tgcaaagcta acccgtggca gcagttcgcg gaaacccaca
     8221 acaagggtga ccgtgttgaa ggtaaaatca agtctatcac tgacttcggt atcttcatcg
     8281 gcctggacgg cggcatcgac ggcctggttc acctgtctga catctcctgg aacgttgcag
     8341 gcgaagaagc agttcgtgaa tacaaaaaag gcgacgaaat cgctgcagtt gttctgcagg
     8401 ttgacgcaga acgtgaacgt atctccctgg gcgttaaaca gctcgcagaa gatccgttca
     8461 acaactgggt tgctctgaac aagaaaggcg ctatcgtaac cggtaaagta actgcagttg
     8521 acgctaaagg cgcaaccgta gaactggctg acggcgttga aggttacctg cgtgcttctg
     8581 aagcatcccg tgaccgcgtt gaagacgcta ccctggttct gagcgttggc gacgaagttg
     8641 aagctaaatt caccggcgtt gatcgtaaaa accgcgcaat cagcctgtct gttcgtgcga
     8701 aagacgaagc tgacgagaaa gatgcaatcg caactgttaa caaacaggaa gatgcaaact
     8761 tctccaacaa cgcaatggct gaagctttca aagcagctaa aggcgagtga ttctctgact
     8821 cttcgggatt tttattccga agtttgttga gtttacttga cagattgcag gtttcgtcct
     8881 gtaatcaagc actaagggcg gctacggccg cccttaatca atgcagcaac agcagccgct
     8941 taatttgcct ttaaggaacc ggaggaatca tgaccaagtc agaattgata gaaagacttg
     9001 ccacccagca atcgcacatt cccgccaaga cggttgaaga tgcagtaaaa gagatgctgg
     9061 agcatatggc ctcgactctt gcgcagggcg agcgtattga aatccgcggt ttcggcagtt
     9121 tctctttgca ctaccgcgca ccacgtaccg gacgtaatcc gaagactggc gataaagtag
     9181 aactggaagg aaaatacgtt cctcacttta aaccaggtaa agaactgcgc gatcgcgcca
     9241 atatttacgg ttaagttttt actcaaactt gaacgcgaga aaagcacctg tcgggtgctt
     9301 ttttcatttc tctaatctgg aactggaagc tgcctcgcag agttttgaac agttttcacc
     9361 ctctcgttaa attcttctga acgtgcctcg gggaacgcaa aactcccacc agacaaccgc
     9421 tcaacaaagt tgcacacttt ccataaatac ggaggggtgc atgaaaataa cgacagtcgg
     9481 cgtatgcata atttgcggaa tttttccgtt gctggttttg ccccaattgc ctgggacatt
     9541 aacccttgcg tttctgactg tcttcgcctg cgtactggca tttattcctg ttaaagccgt
     9601 ccgttatatc gcgctgacgt tgctgttttt tctttggggc atatcagcgg caaagcaaat
     9661 tttgtgggca ggagaaacct taactggcgc gacgcaggat gcaatagttg agatcactgc
     9721 tactgacggc atgaccactc attacggtca agtcactcat ctacaaggtc gacgtatatt
     9781 ccctgcgcca ggcctcgtgc tgtatggcga atatctcccg caagcggttt gtgccggaca
     9841 actatggtca atgaaactca aagttcgtgc agttcatggc caacttaatg atggcagctt
     9901 tgatagccag cgttatgcca ttgcccagca tcagccgctg accggccgct ttctgcaggc
     9961 aagtgtcatt gaaccggatt gtagcttgcg tgcacagtat ctggcgtcat tacaaacaac
    10021 gctgcaaccc tatccgtgga atgcggttat tcttggttta ggtatggggg aacggttatc
    10081 cgttcccaaa gaaatcaaaa atatcatgcg cgatactgga acggcgcatt taatggcgat
    10141 atcgggattg catatcgctt ttgcggcgtt gctggctgcc ggactcattc gcagtggaca
    10201 aatttttctg cctgggcgct ggatccactg gcaaatgcca ttaattggcg gaatctgctg
    10261 tgctgctttt tatgcctggc tgacgggaat gcaacctcct gcatttcgta ccgtggtggc
    10321 gcttgccatg tggggaatgc ttaagttaag tgggcgacaa tggagtggct gggatgtatg
    10381 gatatgttgt ctggcggcaa ttttgctgat ggatcctgtt gccattctct cgcaaagttt
    10441 atggctctct gccgctgcgg tcgcggcact gattttttgg tatcagtggt ttccctgtcc
    10501 tgagtggcag ctgccgccgg tattgcgtgc ggttgtttcc ctcatccatc tgcaactggg
    10561 aatcaccctc ctgcttatgc ccgtgcaaat cgtcatattt catggcatta gtctgacctc
    10621 gtttattgca aatctatttg caattcccct ggtgacattt atcacggttc cattgatcct
    10681 cgccgcgatg gttgtgcatt taagcgggcc gttaatcctg gaacaggggt tatggtttct
    10741 tgccgaccgg tctttggctt tacttttctg ggggttaaag agtttgccgg aagggtggat
    10801 caacattgct gaacgttggc aatggctaac attttcccca tggttcttac tggtggtatg
    10861 gcgattaaac gcctggcgaa cgttgccagc aatgtgtgtg gctggaggct tgctgatgtg
    10921 ctggccgctg tggcaaaaac ctcgacctga cgagtggcaa gtgtacatgc ttgatgtcgg
    10981 gcaagggctg gcaatggtca ttgcgcgaaa cggcaaagcg attctctatg acacgggact
    11041 ggcctggccc gaaggggata gtgggcaaca actgattatc ccctggctcc actggcataa
    11101 tcttgaaccg gaaggcgtta ttctgagtca tgaacatctg gatcaccggg gagggctgga
    11161 ctcaatattg cacacatggc cgatgttatg gatcagaagt ccgttaaact gggaacatca
    11221 tcagccctgt gtgcgtggcg aagcgtggca atggcaagga ttgcgtttca gcgcgcactg
    11281 gcctttacaa gctagcaacg ataaaggaaa taaccattcc tgtgtggtta aggttgatga
    11341 cgggacgaat agcattcttc taaccggtga tattgaagcc cccgctgaac aaaagatgct
    11401 aagccgttac tggcagcaag tgcaggcaac attgcttcag gtacctcacc atggcagtaa
    11461 tacctcatca tcgttgccat taattcagcg agtgaatgga aaagtggcac tcgcatcggc
    11521 atcgcgctat aacgcatggc gattgccctc caacaaagtt aagcatcgct atcaacaaca
    11581 aggttataag tggcttgata ctccacatca gggtcaagtg acggtcaatt tttcagcgca
    11641 aggctggcgg attagcagcc tcagggagca aattttacct cgttggtatc atcagtggtt
    11701 tggcgtgcca gtggataacg ggtagaatat gcggctattt caacaaatgc tggttttttg
    11761 aatgcataac gacaaagatc tctctacgtg gcagacattc cgccgactgt ggccaaccat
    11821 tgcgcctttc aaagcgggtc tgatcgtggc gggcgtagcg ttaatcctca acgcagccag
    11881 cgataccttc atgttatcgc tccttaagcc acttcttgat gatggctttg gtaaaacaga
    11941 tcgctccgtg ctggtgtgga tgccgctggt ggtgatcggg ctaatgattt tacgtggtat
    12001 caccagctat gtctccagct actgtatctc ctgggtatca ggaaaggtgg taatgaccat
    12061 gcgtcgccgc ctgtttggtc acatgatggg aatgccagtt tcattctttg acaaacagtc
    12121 aacgggtacg ctgttgtcac gtatcaccta cgattccgaa caggttgctt cttcttcttc
    12181 cggcgcactg attactgttg tgcgtgaagg tgcgtcgatc atcggcctgt tcatcatgat
    12241 gttctattac agctggcaac tgtcgatcat tttgattgtg ctggcaccga ttgtttcgat
    12301 tgcgattcgc gttgtatcga agcgttttcg taacatcagt aaaaacatgc agaacaccat
    12361 ggggcaggtg accaccagcg cagaacaaat gctgaagggc cacaaagaag tattgatttt
    12421 cggtggtcag gaagtggaaa cgaaacgctt cgataaagtc agtaaccgaa tgcgtcttca
    12481 ggggatgaaa atggtttcag cctcttccat ctctgatccg atcattcagc tgatcgcctc
    12541 tttggcgctg gcgtttgttc tgtatgcggc gagcttccca agtgtcatgg atagcctgac
    12601 tgccggtacg attaccgttg ttttctcttc aatgattgca ctgatgcgtc cgctgaaatc
    12661 gctgaccaac gttaacgccc agttccagcg cggtatggcg gcttgtcaga cgctgtttac
    12721 cattctggac agtgagcagg agaaagacga aggtaagcgc gtgatcgagc gtgcgactgg
    12781 cgacgtggaa ttccgcaatg tcacctttac ttatccggga cgtgacgtgc ccgcattgcg
    12841 taacatcaac ctgaaaattc cggcagggaa gacggttgct ctggttggac gctctggttc
    12901 aggtaaatca accatcgcca gcctaatcac acgtttttac gatattgatg aaggcgaaat
    12961 cctgatggat ggtcacgatc tgcgcgagta taccctggcg tcgttacgta accaggttgc
    13021 tctggtgtcg cagaatgtcc atctgtttaa cgatacggtt gccaacaaca ttgcttacgc
    13081 acggactgaa cagtacagcc gtgagcaaat tgaagaagcg gcgcgtatgg cctacgccat
    13141 ggacttcatc aataagatgg ataacggtct cgatacagtg attggtgaaa acggcgtgct
    13201 gctctctggc ggtcagcgtc agcgtattgc catcgctcga gccttgttgc gtgatagccc
    13261 gattctgatt ctggacgaag ctacctcggc gttggatacc gaatccgaac gtgcgattca
    13321 ggcggcactg gatgagttgc agaaaaaccg tacctctctg gtgattgccc accgcttgtc
    13381 taccattgaa aaggcagacg aaatcgtggt cgtcgaggat ggtgtcattg tggaacgcgg
    13441 tacgcataac gatttgcttg agcaccgtgg cgtttacgcg caacttcaca aaatgcagtt
    13501 tggtcaatga tcgaaaaaat ctggtctggt gaatcccctt tgtggcggct attgctgcca
    13561 ctctcctggt tgtatggcct ggtgagtggc gcgatccgtc tttgctataa actaaaactg
    13621 aagcgcgcct ggcgtgcccc cgtaccggtt gtcgtggttg gtaatctcac cgcaggcggc
    13681 aacggaaaaa ccccggtcgt tatctggctg gtggaacagc tgcaacagcg cggtattcgc
    13741 gtgggggtcg tatcgcgggg atatggtggt aaggctgaat cttatccgct gttattgtcg
    13801 gcagatacca ccacagcaca ggcgggtgat gaacctgtgt tgatttatca acgcactggc
    13861 gcgcccgttg cggtttctcc cgtgcgttct gatgcggtaa aagccattct ggcgcaacac
    13921 cctgatgtgc agatcatcgt aaccgacgac ggtttacagc attaccgtct ggcgcgtgat
    13981 gtggaaattg tcgttattga tggtgtgcgt cgctttggca atggttggtg gttgccggcg
    14041 gggccaatgc gtgagcgagc ggggcgctta aagtcggttg atgcggtaat cgtcaacggc
    14101 ggtgtccccc gcagcggtga aatccccatg catctgctgc cgggtcaggc ggtgaattta
    14161 cgtaccggta cgcgttgtga cgttgctcag cttgaacatg tagtggcgat ggcggggatt
    14221 ggacatccgc cgcgcttttt tgccacgctg aagatgtgcg gcgtacaacc ggaaaaatgt
    14281 gtaccactgg ccgatcatca gtctttgaac catgcggatg ttagtgcgtt ggtaagcgcc
    14341 gggcaaacgc tggtaatgac tgaaaaagat gcggtgaaat gcctgccctt tgccgaagga
    14401 aattggtggt atttgcctgt tgacgcacag ctttcaggtg atgaaccagc gaaactgctt
    14461 gcgcaactaa cctcgctggc ttctggcaac tagttacgcc gcggcagcgt tcgattgatg
    14521 gagtcatgaa tgtcgctgcc gcacctctct cttgctgatg cgcgtaatct tcacctcgcc
    14581 gcgcaaggcc tgttaaacaa accccgccgt cgagcatcgt tggaggatat tccggcaacg
    14641 atctcccgca tgtccttgct gcaaatcgat accattaata ttgttgcccg tagtccatat
    14701 ctggtgcttt tcagtcgtct gggaaattat cctgcccagt ggctggatga gtctctggcg
    14761 cgtggcgaat taatggaata ctgggcgcat gaagcctgct ttatgccgcg tagcgacttt
    14821 cgtcttattc gccaccgcat gctggcacct gaaaaaatgg gctggaaata caaaaacgcc
    14881 tggatgcagg aacatgaggc ggaaattgca cagttaattc agcatattca tgataagggg
    14941 ccggtacgtt cagccgattt cgagcattct cgtaaaggtg caagcggctg gtgggaatgg
    15001 aagccgcata aacggcatct ggaaggttta tttactgccg gaaaggtgat ggtgattgaa
    15061 cggcgcaact tccagcgcgt ttatgattta acccaccgag tcatgcctga ctgggatgat
    15121 gagcgcgatc tcgtttcgca aacagaagca gaaatcatca tgctggataa cagtgcgcgt
    15181 agcctgggaa tattccgcga acagtggctg gcagattact atcggctgaa acgtccggca
    15241 ctggcggcgt ggcgcgaagc gagggctgaa cagcagcaaa tcattgctgt gcatgttgaa
    15301 aaattgggca atctttggct gcatgctgat ttgctgccgc tactcgagcg tgcgctggcc
    15361 ggaaagctca ctgcaacgca cagcgcggta ctttcgcctt ttgatcctgt tgtctgggat
    15421 cgcaaacgcg cagagcagct ttttgatttt agctaccggc tggagtgcta taccccagcg
    15481 ccgaaacgcc agtatggcta ttttgttctg ccgttattac atcgtgggca attagttggg
    15541 cgaatggatg ccaaaatgca tcgccagaca ggcatccttg aagttatctc tctgtggtta
    15601 caagaaggca ttaaaccaac gacaacgctg caaaaagggt tacgtcaggc gattactgat
    15661 ttcgctaact ggcagcaggc aacgcgggtg acattagaac gctgcccgca aggcctcttt
    15721 actgattgcc gcaccggctg ggaaatagac cccgtcgcat aaatgattat gataagattt
    15781 atgcattcat aacccgatcc atctggagga actatggatc atcgtctgct tgaaatcatt
    15841 gcctgcccgg tttgcaacgg aaaactttgg tataaccagg aaaaacaaga acttatttgc
    15901 aaactggaca acctggcttt cccgctgcgc gatggcattc cagtgttgct ggaaacggaa
    15961 gcccgcgtgc tgactgctga tgagagtaaa tcatgagttt tgtggtcatt attcccgcgc
    16021 gctacgcgtc cacgcgtctg cccggtaaac cattggttga tattaacggc aaacccatga
    16081 ttgttcatgt tcttgaacgc gcgcgcgaat caggtgccga gcgcatcatc gtggcaaccg
    16141 atcatgagga tgttgctcgc gctgttgaag ccgctggcgg tgaagtatgt atgacgcgcg
    16201 ccgatcatca gtcaggaaca gaacgtctgg cggaagttgt cgaaaaatgc gcattcagcg
    16261 acgacacggt gatcgttaat gtgcaggggg atgaaccgat gatccctgcg acaatcattc
    16321 gtcaggttgc tgataatctc gcccagcgtc aggtgggtat ggcgactctg gcggtgccaa
    16381 tccacaatgc ggaggaagcg tttaacccga atgcggtgaa agtggtcctc gacgctgaag
    16441 ggtatgcact gtacttctct cgcgctacca ttccttggga tcgtgatcgt tttgcaaaag
    16501 gccttgaaac cgttggcgat aacttcctgc gtcatcttgg tatttatggc taccgtgcag
    16561 gctttatccg tcgttacgtc aactggcagc caagtccgtt agaacacatc gaaatgttag
    16621 agcagcttcg tgttctgtgg tacggcgaaa aaatccatgt tgctgtcgcc caggaagttc
    16681 ctggcacagg tgtggatacc cctgaagatc tcgagcgcgt tcgcgctgaa atgcgctaat
    16741 ttcacttcac gacactccag ccaattttgg gaggagtgtc gtaccgttac gattttcctc
    16801 aatttttctt ttcaacaatt gatctcattc aggtgacatc ttttatattg gcgctcatta
    16861 tgaaagcagt agcttttatg agggtaatct gaatggaaca gctgcgtgcc gaattaagcc
    16921 atttactggg cgaaaaactc agtcgtattg agtgcgtcaa tgaaaaagcg gatacggcgt
    16981 tgtgggcttt gtatgacagc cagggaaacc caatgccgtt aatggcaaga agctttagta
    17041 cgcccggaaa agcccgacaa ctggcatgga aaaccaccat gctggcaaga agtggaactg
    17101 tccgtatgcc gactatttat ggtgtgatga cgcatgaaga acaccccggc cccgatgtcc
    17161 tgctactgga gcggatgcgt ggtgtttcgg tggaggcacc agcccgaaca ccagaacgct
    17221 gggaacagct caaagatcaa atcgttgaag ccttactggc ctggcaccgt caggacagtc
    17281 gcggttgcgt cggcgcggtc gacaatactc aggaaaattt ctggccctca tggtaccggc
    17341 aacatgttga agtgctatgg accacgctca atcagttcaa taacaccggt ctaacgatgc
    17401 aggataagcg gatcctgttt cgtactcgcg aatgtcttcc ggcattattt gaaggcttta
    17461 acgacaattg tgtgctgatt cacggtaact tctgtttacg cagcatgctg aaagattcgc
    17521 gcagcgatca gttactggcg atggtcgggc cgggactaat gctttgggca ccgcgagaat
    17581 acgaactgtt ccgactaatg gataattctc tggcggaaga tttgctctgg agttacctgc
    17641 aacgcgcgcc agtggcggag tcgttcatct ggcggcgttg gttgtatgtg ttatgggatg
    17701 aagttgcgca actggttaat accggacgat ttagtcggcg caacttcgat ctggcatcaa
    17761 aatcactctt gccgtggctc gcctgacgga cctttcagcc actgccagat gcggccaagc
    17821 gtttcatagc caacacgatc gctatgcatc aaccaaaccg gagaagggat tgcccgttcc
    17881 cacggattaa gcggcgagtc gatggccaac tgattcgctg gggctggtag cggatttaac
    17941 ccttcctgct gaaaaaagat catcgcccgc ggcagatgtg atgcggaggt caccagcagg
    18001 aaaggggcgt caccaatcgc ctgtttcact gccgcagctt cttcttcggt atcttttggc
    18061 aaatccagag tgataatttg ctcgcgcggc acgcccagcg attgcgcaac tctggcacct
    18121 acttccgctg tactcaccgt attggttttt gctacgcctc ccgtgaagat cagttttgat
    18181 cccggatttt cgcgccataa gcgaatacct tcattcaggc gaggcagact gttattgatt
    18241 aaattcgagc ttggtgccca ctgcgggttc caggtataac cacctccgag caccacgata
    18301 tagtccactt tttgcgaatt attccatgtc ggataggtgc tttcgatggg acgcagcagg
    18361 cggtcggcta ccggttgcaa actcaataac aagagcgcca gccacccaat gctaataaaa
    18421 atcttcccgg ttttttgaaa acggctaaac cacagcagcg ccaggccagc gccaataatc
    18481 aacaacatca gcggaagtgg cagcaacata ttgccaatca cttttttcag tgtaaaaagc
    18541 atcctttctg gttccttttt taaccatata gcaagggatc gccagcgata ttacaccaga
    18601 caggttcatt ctcgccgtgg ctgtgacaaa atagcgtctt tatcagaaaa cgtcttagcc
    18661 aaccggtgga gaaagcaatg caggatcgca attttgatga tattgcggaa aagttttccc
    18721 gtaacattta cggcaccacc aaagggcagc ttcgacaggc tattctgtgg caggatctcg
    18781 atcgcgtgct ggcggaaatg ggcccgcaaa aactgcgtgt gctggatgct ggcggtggag
    18841 aagggcagac cgcaatcaaa atggccgagc gtgggcatca ggtcatttta tgcgatcttt
    18901 ctgcgcagat gatcgaccgc gcaaaacagg cggcagaagc aaaaggtgtg agcgacaaca
    18961 tgcaatttat acattgcgcc gctcaggatg ttgcttcgca tttggaaacg cccgttgatc
    19021 tgatattgtt ccatgcggtg ctcgagtggg tggccgatcc ccgcagcgta ttacagaccc
    19081 tgtggtcagt gttgcgtcca ggcggtgtgt tgtcgttaat gttctacaat gcgcacggct
    19141 tgctgatgca taacatggtc gccgggaatt ttgattacgt gcaggcggga atgccgaaaa
    19201 agaaaaaacg gacgctttcg ccagattatc cacgcgaccc ggcgcaggtt tatctgtggc
    19261 tggaagaagc tggttggcaa attatgggta agacaggcgt tcgcgtgttt catgattatc
    19321 tgcgcgagaa gcaccagcag cgcgactgct atgaagcatt acttgaatta gaaacgcgtt
    19381 attgccgtca ggaaccgtat attaccctgg ggcgttatat tcatgtcacc gcgcgcaaac
    19441 cgcagagcaa ggataaagta tgagtgaatt ttcccagaca gtccccgaac tggttgcctg
    19501 ggccagaaaa aatgacttct ccatctcgct gccggtagac cgactctctt ttctgctggc
    19561 ggttgccacg ctgaacggcg agcgtctgga tggtgagatg agtgaaggcg agctggtgga
    19621 tgcattccgc catgtgagtg atgcgtttga gcaaaccagc gaaaccatcg gcgtgcgcgc
    19681 caacaacgcg atcaacgaca tggtgcgtca acgtctgctg aaccgcttta ccagcgagca
    19741 ggcggaaggg aacgcaattt accgtctgac gccgctcggc atcggcatta ctgactatta
    19801 catccgtcag cgcgagtttt ctacgctgcg cctttctatg cagttgtcga ttgtggcggg
    19861 tgagctcaaa cgcgcagcag atgccgccga agagggcggt gatgaatttc actggcaccg
    19921 taatgtctat gcgccactga aatattcggt agcagaaatt tttgacagta tcgacctgac
    19981 gcaacgtctg atggacgaac agcagcagca ggtgaaggac gatatcgccc agttgctgaa
    20041 caaagactgg cgggcggcga tctccagctg tgaattgttg ctttcggaaa cttccggaac
    20101 gctgcgtgaa ttgcaggata cgctggaagc ggcaggcgac aaattgcagg ctaatctgtt
    20161 gcgcattcag gatgcgacga tgacccatga cgatctgcat tttgtcgatc gtctggtatt
    20221 cgatctgcag agcaaactcg accgtattat cagctggggc cagcaatcca tagacttgtg
    20281 gattggctac gaccgccacg tacacaaatt tatccgtacc gcaatcgata tggataaaaa
    20341 ccgcgtcttt gctcagcggt tacgtcagtc ggtacaaacc tattttgatg agccgtgggc
    20401 gctaacttat gccaatgccg atcgtctgtt ggatatgcgt gacgaagaga tggctctgcg
    20461 cgatgaagaa gtgacaggag aacttcctga ggatctggag tacgaagagt ttaacgagat
    20521 ccgcgaacag ctggcggcga tcatcgaaga acaactcgcc gtgtacaaaa ccagacaagt
    20581 gccgctggat cttggtctgg tggtacgcga atatctgtca cagtatccgc gtgcacgtca
    20641 ctttgacgtt gcgcgcattg ttattgatca ggcggtacgt cttggcgtag cgcaagcaga
    20701 tttcaccgga ctgccagcga aatggcagcc gattaatgat tacggagcca aggtacaggc
    20761 gcatgtcatc gacaaatatt gaacaagtga tgccggttaa gctggcgcag gcgctggcga
    20821 atccgttatt tccggcgctg gatagcgcct tacgttcagg ccgccatatt ggcctcgacg
    20881 aactggataa tcatgcattc ctgatggatt ttcaggaata tctggaagag ttttacgcgc
    20941 gttataacgt tgagcttatt cgtgcaccgg aagggttctt ctatttacgc ccacgttcca
    21001 ccacgctgat ccctcgttcc gtcttgtcgg aactggatat gatggtcggg aaaatcctct
    21061 gttatctcta tctcagcccg gaacggctgg cgaatgaggg gatttttact caacaggaac
    21121 tgtacgacga actgctcacc ctggccgatg aagcgaaact gctgaaactg gtgaacaacc
    21181 gttcaaccgg ttcagacgtt gaccggcaga agttgcagga gaaagtacgt tcttcgctca
    21241 accgtctgcg tcgtttgggc atggtgtggt ttatgggcca cgacagcagc aagttccgca
    21301 ttaccgaatc ggtgttccgc ttcggagccg atgtgcgtgc tggcgacgat ccccgtgaag
    21361 cacagcgtcg cctgattcgt gatggcgaag caatgccgat tgaaaatcat ctgcaactca
    21421 acgatgaaac cgaagagagt cagccagata gcggagagga agaataatga ttgaacgcgg
    21481 taaatttcgc tcactgacgc tgattaactg gaacggcttt tttgcccgaa cttttgacct
    21541 tgacgagctg gtcacgacgc tttctggcgg taacggggcg ggtaaatcca ccaccatggc
    21601 ggcgttcgtt acggcgctga tccccgacct gaccctgctg catttccgta acactacgga
    21661 agccggggcc accagcggtt cgcgtgataa aggtctgcac ggtaagctga aagcgggtgt
    21721 ctgttattcg atgctcgaca ccattaactc gcgccaccag cgcgtggtgg tcggtgtgcg
    21781 tctgcaacag gttgccggac gcgatcgtaa agtggatatc aaaccatttg ccattcaggg
    21841 cctgccgatg tcggtgctgc cgacgcagtt ggtgaccgaa accctgaacg aacgccaggc
    21901 gcgcgtgctg ccgcttaacg agctgaaaga caagctcgag gcgatggaag gcgtgcagtt
    21961 taaacagttc aactccatta ccgattacca ctcgctgatg ttcgatctgg gcatcatcgc
    22021 gcgtcgtctg cgctccgcat ctgaccgtag caaattctat cgtctgatcg aagcctcgct
    22081 gtatggcggg atctccagcg ccattactcg ttctctgcgc gactacctgt tgccggaaaa
    22141 cagcggggtg cgtaaagcgt tccaggacat ggaagcggca ctgcgtgaaa atcgcatgac
    22201 gctggaagcg attcgtgtca cccagtcgga ccgtgacctg tttaagcatc tgatcagcga
    22261 agccaccaac tacgtggcgg cggactacat gcgtcacgcc aacgagcgcc gtgtccatct
    22321 cgacaaagcc ctggaatttc gtcgcgagct acatacttcg cgtcagcaat tggcggctga
    22381 gcagtacaaa cacgtcgata tggcgcgtga gctggcagag cacaacggtg ccgaaggcga
    22441 tctggaagcg gattatcagg cggccagcga tcacctgaac ctggtgcaaa ccgcactgcg
    22501 tcagcaggag aagatcgaac gttacgaagc ggatctcgat gagctgcaga tccgtctgga
    22561 agagcaaaac gaagtggtgg cagaagccat cgaacgccag gaagagaacg aggctcgtgc
    22621 ggaagctgcc gaactggaag tggacgagct gaaaagccag cttgctgact atcagcaggc
    22681 gctggacgtc cagcaaacgc gcgcgatcca gtataaccag gcgattgctg cgcttaatcg
    22741 tgccaaagaa ctgtgccatc tgccggactt aaccgccgac agcgccgccg aatggctgga
    22801 aaccttccag gcgaaagagc tggaagcgac tgaaaaaatg ctctctcttg agcagaaaat
    22861 gagcatggcg caaaccgcgc acagccagtt tgagcaggct tatcagctgg tggtggcaat
    22921 taatggcccg ctggcgcgta acgaggcgtg ggatgtcgct cgcgaactat tgcgcgaagg
    22981 ggtcgatcag cgtcacctgg cagagcaggt tcaaccgttg cggatgcgct taagcgaact
    23041 ggaacagcgt ctgcgcgagc agcaagaagc cgaacgcctg ctggcagatt tctgcaaacg
    23101 tcagggcaag aattttgata ttgatgaact ggaagctctg catcaggagc tggaagcacg
    23161 cattgcctct ctttccgata gcgtgtctaa cgcccgcgaa gagcgcatgg cactgcgcca
    23221 ggaacaggaa cagctgcagt ctcgcattca gagtttgatg cagcgtgcgc cggtttggct
    23281 ggcagcgcaa aacagtctca accagttgag cgaacagtgc ggcgaagagt tcacctccag
    23341 ccaggacgtc accgaatatc tgcaacagtt gcttgagcgt gagcgcgaag cgattgttga
    23401 acgtgacgaa gttggcgcac gcaaaaacgc cgtcgatgaa gagatcgaac gtttaagcca
    23461 gccaggcggc tctgaagatc agcgtctgaa cgcgctggcg gagcgttttg gtggtgtgct
    23521 gctgtcagaa atttatgatg acgttagcct ggaagatgcg ccgtacttct cagcgctgta
    23581 tggcccgtca cgccacgcca tcgtggtgcc agatctgtca caggtaaccg agcacctgga
    23641 aggcttgacc gattgcccgg aagatctcta tctgatcgaa ggggatccgc agtcattcga
    23701 tgacagcgtg ttcagcgttg atgagctgga aaaagctgta gtggtgaaaa tcgccgatcg
    23761 tcagtggcgt tattcacgtt tcccggaagt gccgctgttt ggtcgtgctg cgcgtgaaag
    23821 ccgtattgaa agcctccatg ccgagcgtga agtgctttcc gaacgcttcg ccacgctctc
    23881 ctttgatgta cagaaaaccc agcgtctgca tcaggcattc agccgcttta taggtagtca
    23941 tctggcggtg gcgtttgagt ctgacccgga agcagaaatc cgtcaactga acagccgtcg
    24001 cgtcgaactg gagcgggcgt tgagtaatca tgaaaatgat aaccagcagc agcgtattca
    24061 gtttgagcag gcgaaagagg gtgttacggc gctgaaccgc attctgccgc gtctcaactt
    24121 gttggctgat gacagcctgg cggatcgcgt cgatgaaatc cgcgaacgtc tggatgaagc
    24181 ccaggaagcc gcgcgttttg ttcagcagtt cggtaatcaa ctggcgaaac tggaaccgat
    24241 cgtttcggta ttgcagagtg acccggaaca gttcgaacag ttaaaagaag attacgcgta
    24301 ctctcagcag atgcagcgcg atgcccgtca gcaggcgttt gccctgacgg aagtggtgca
    24361 gcgtcgtgcg cactttagct actctgactc ggcagaaatg cttagcggta acagcgatct
    24421 caacgaaaaa ctgcgtgaac gtctggaaca ggcggaagcg gagcgtaccc gcgcgcgtga
    24481 agcgttgcgc ggacacgcag cgcagttgag tcagtacaac caggtgctgg cttcgctgaa
    24541 aagttcttac gacaccaaaa aagagctact caatgatctg caacgtgaat tgcaggatat
    24601 cggcgtgcga gctgatagcg gggcagaaga gcgggcgcgt attcgccgtg acgagttgca
    24661 cgcgcaactg agcaataacc gttcacgccg caatcaactg gaaaaagcgc ttaccttctg
    24721 cgaagcagag atggacaacc tgacccgcaa actgcgcaag ctggagcggg attactttga
    24781 gatgcgcgag caggtagtga ccgcgaaagc gggctggtgt gcagtcatgc gcatggtgaa
    24841 agataacggc gttgagcgcc gcttacaccg tcgtgagctg gcttatctct ccgctgatga
    24901 tttgcgttcc atgtcggata aggcgttagg tgcgctgcgt ctggcagtgg cagataacga
    24961 acatctgcgc gatgtgctgc gcatgtcgga agatccaaaa cgtccggaac gtaaaattca
    25021 gttcttcgtg gcggtttatc agcatctgcg tgaacgtatt cgtcaggata ttattcgtac
    25081 cgatgatccg gtggaagcta tcgaacagat ggagatcgaa cttagccgtc tgaccgaaga
    25141 gttaacctcc cgtgaacaga aactggcgat cagttcccgc agcgtggcga acattattcg
    25201 aaaaaccatt cagcgcgagc agaaccgtat ccgtatgctc aaccaggggt tgcagaacgt
    25261 atcgtttggt caggtgaaca gcgtgcgcct caacgtgaac gtgcgtgaaa cgcacgccat
    25321 gctgctggat gtgctctctg aacagcatga gcagcatcag gatctgttta acagcaaccg
    25381 tctgaccttc tcggaagcgc tggcgaaatt gtatcagcgt cttaatccgc agattgatat
    25441 ggggcagcgc acgccgcaga ccattggcga agaactgctg gattaccgca actatctgga
    25501 aatggaagtt gaggttaacc gtggttctga tggctggttg cgcgcagagt ctggtgcatt
    25561 gtcgaccggt gaggcgattg gtaccggtat gtcgattctg gtgatggtgg tacaaagctg
    25621 ggaagatgaa tctcgccgcc tgcgcggtaa agatatctct ccttgccgcc tgctgttcct
    25681 cgatgaagca gcgcgactgg atgctcgttc tatcgccacg ctgtttgaat tgtgtgaacg
    25741 tttgcaaatg cagctcatca tcgcagcgcc ggaaaatatc agcccggaga aaggcaccac
    25801 ctataaactg gtgcgtaaag tcttccagaa taccgaacac gttcacgtcg tcggcctgcg
    25861 tggctttgcg ccgcaactcc ctgaaacgct tccaggaagt gacgaagcgc cttctcaggc
    25921 gagttaaaat taaggcggca gcaatgccgc cttttctttt tccgaaaact ccgtttctgc
    25981 actaaaaaag tggcacatta cggcgcgctt ttctttaaac ttctttacat taggttatgt
    26041 aaaaacgtat cggcgtttat atactgaaga taagcctgat gagtaacagg cttgctcgtc
    26101 atactttcgt gagtattggc gttgtacagg caagtcgtaa aataacagcc tggctattca
    26161 gagtatgata aaaacagggg gcaagggatg ttgcttaata tgatgtgtgg tcgtcggctg
    26221 tcggcaatca gtttgtgcct ggccgtaaca ttcgctccac tgttcaatgc gcaggccgat
    26281 gagcctgaag taatccctgg cgacagcccg gtggctgtca gtgaacaggg cgaggcactg
    26341 ccgcaggcgc aagccacggc aataatggcg gggatccagc cattgcctga aggtgcggca
    26401 gaaaaagccc gcacgcaaat cgaatctcaa ttacccgcag gttacaagcc ggtttatctt
    26461 aaccagcttc aactgttgta tgccgcacgc gatatgcaac ccatgtggga aaaccgtgat
    26521 gctgttaaag ccttccagca acagctggcc gaggtggcga ttgccggttt ccagccgcag
    26581 tttaataaat gggtagagtt actgaccgat cctggtgtta acggaatggc acgcgacgtc
    26641 gtgctctctg atgcgatgat gggctatctc catttcattg caaatattcc ggtcaaaggc
    26701 actcgctggc tatatagcag taaaccttat gcgcttgcaa cgccgccgct ttcggtgatt
    26761 aaccaatggc agctggcgct ggataaaggt caattgccga cgtttgttgc aggactggca
    26821 ccgcagcatc cgcaatatgc ggcgatgcat gaatcgttac tggccttact cagtgacacc
    26881 aaaccgtggc ctcaactgac cggtaaagca acgttgcgcc cagggcagtg gagtaacgac
    26941 gtaccggcgt tgcgcgaaat attgcaacgc acaggcatgt tggacggggg gccaaaaatt
    27001 actctccctg gcgatgacac gccaactgac gcagtagtca gcccatccgc tgttactgtt
    27061 gaaacagcag aaactaagcc gatggacaag caaacgacgt ctcgtagtaa acctgcgcct
    27121 gccgttcgtg ccgcctacga taatgaactg gtggaggccg ttaaacgttt tcaggcatgg
    27181 caaggattgg gagcagatgg cgctattggc ccggcaacgc gtgactggtt aaacgtaacg
    27241 cccgcccagc gtgctggcgt gttggcactc aacatccagc gattgcgctt gctaccaaca
    27301 gagctttcta ccgggatcat ggttaacatt ccagcctatt cgctggtcta ctatcagaac
    27361 ggcaatcagg tactggattc gcgagtcatt gtcggtcgcc ccgatcgcaa aacgccgatg
    27421 atgagcagtg cacttaacaa cgtagtggta aacccgccgt ggaacgtacc accaactctg
    27481 gcacgcaaag atattctgcc gaaagtgcgc aacgatccgg gatacctcga aagccatggc
    27541 tatacggtga tgcgcggctg gaacagcaga gaagcgattg acccatggca ggttgactgg
    27601 tctacaatca cggcctcgaa tttaccgttt cgcttccagc aggctccagg cccacggaac
    27661 tcgctggggc gctataaatt caatatgccg agttcagagg ccatttattt gcatgacacg
    27721 ccgaaccaca atctgttcaa gcgtgataca cgcgcattga gctcaggctg tgtacgagtg
    27781 aataaagctt ccgatctggc gaatatgctg ttgcaggatg caggatggaa tgacaaacgt
    27841 atttctgatg cgctgaagca gggcgatacg cgttacgtca atattcggca gtcgattccg
    27901 gtgaatctct actacctgac ggcctttgtt ggtgcagatg gtcgtaccca gtatcgtaca
    27961 gatatttaca attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa
    28021 caattaatca ggtaaatgaa gcagttcggg gaaatcagtt gtcgtaataa ttagcatgat
    28081 cgggggcgat tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtcagctt
    28141 tactgacggt taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgacct
    28201 gtagacttga ttatcatgga caaattcgac gctaatcgcc gcaaactgct ggcgcttggt
    28261 ggcgttgcac tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca
    28321 cgcccgcgta ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt
    28381 ttcgatggca gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat
    28441 tatcgcgcga acaaaataaa gtccatcgac ccaggattat tcgatcagtt gtatcgcctg
    28501 caagggttgt taggcacccg caaaccggtg caactcattt ccggttatcg ctccattgat
    28561 accaacaatg aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacacgaaa
    28621 gggcaggcga tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg
    28681 ttatctatgc gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat
    28741 accgggccag cacggcactg gtagtaattg cttaacgaaa caggggcagt atgaactatc
    28801 gtattattcc ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc
    28861 gtctggccgc actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgacg
    28921 ccagcggcct gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg
    28981 cagcggcgga actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg
    29041 agttctggct gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagc
    29101 cgctgacgcc agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtggctt
    29161 tacaggtgtt acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg
    29221 caaagctgct gatttctggc gacgttattt tcaaaggcgg agtagggcgc agtgacttcc
    29281 cgcgtggcga tcataatcaa ctgatttctt caatcaaaga taagttgctg ccactggggg
    29341 atgacgtgac atttattccg ggtcacggac cattatccac acttggttat gagcgcctgc
    29401 ataatccatt cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagcccgctt
    29461 tttaagcggg cttcattgtt tttaacgctt acagcactgc cacaatcgct tcgcacagcg
    29521 gagccatgtt atctggtgtc atcccggcta cgttcacacg accagaagca acagcataca
    29581 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt cagaccactg aaggagaaca
    29641 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
    29701 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
    29761 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
    29821 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
    29881 tttcactgtc agcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
    29941 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
    30001 cttcagcatc ttcttccaga ccacgggcaa acccctggta agcgaagtca aacagcggta
    30061 accagccttt ctcaacggaa agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
    30121 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
    30181 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtgatt ttccgcatca taataagcgt
    30241 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
    30301 tgctcaccca cacacgctta acgctggtat tttttgccag gaaatcggca gccacgcgta
    30361 gtgcgccagt gccacccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
    30421 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
    30481 cgaggtaatt tttggtggtt tcattttcga gcaaatactg ttcagccttt ttcacgctgg
    30541 tcagtaccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
    30601 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
    30661 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
    30721 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
    30781 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaacgtcc tgttttttcg
    30841 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
    30901 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
    30961 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
    31021 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagctat ggacggacgc
    31081 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
    31141 ccgccttcaa ttggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
    31201 ttcgcgtcat acttcagacc agttgcccac tgttcagctt ttttaccctg accgcggaat
    31261 tcggcttcct gggcgtcggt acggtctgct gcaccgtaag cacctacgat accaaagcct
    31321 tcgaattcgt agctgataga gccaccgacg ccgtcgccgt tggaacgctc aggaatacca
    31381 gcacgttcgt ttttgcccag gtactgaacg gcaaagttca ggccgtcaac cagaccgaag
    31441 aagttggagt tacgataggt agcaacgccg cctacacgac caacgaagaa gtcatcgctg
    31501 tatgccgtgt caccgccaaa ctcaggcagc atatcggtgt aacccagtgc gtcgtaaacc
    31561 acaccgtagt tacgaccgta gtcgatagaa cccgcatcag cgaatttcag acctgcgaat
    31621 gccagacgag ttttgttacc tttctgggca tcagcgcctt cagagttgtt accctggaag
    31681 ttatattccc actgaccgta gccggtcaga tcggaattga tttgtgtttc ccctttaaaa
    31741 ccaagacggg cataagtttt gtcgccgttt ccgccgtaac tgtttacacc attgtcttta
    31801 gaaaaataat gcagaccgac agctttaccg tacagatcta ctttgttgcc atctttgtta
    31861 tagatttctg cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga
    31921 atattgcgct tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc
    31981 cgtcaataag ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta
    32041 tgtgtctgca ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa
    32101 gataaaaatt tggtttcaaa aagaaaaaat atgtaaccaa aagtaaa
//
LOCUS       DARNVM010000038        32093 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.038, whole genome shotgun sequence.
ACCESSION   DARNVM010000038 DARNVM010000000
VERSION     DARNVM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.038"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(243..2816)
                     /gene="clpB"
                     /locus_tag="SI621_001603"
     CDS             complement(243..2816)
                     /gene="clpB"
                     /locus_tag="SI621_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEI4064334.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2946..3677)
                     /gene="pgeF"
                     /locus_tag="SI621_001604"
     CDS             complement(2946..3677)
                     /gene="pgeF"
                     /locus_tag="SI621_001604"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HEI4064335.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGRDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADISLLARQRLANVGVEQIFGGNRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3674..4654)
                     /gene="rluD"
                     /locus_tag="SI621_001605"
     CDS             complement(3674..4654)
                     /gene="rluD"
                     /locus_tag="SI621_001605"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEI4064336.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4789..5526
                     /gene="bamD"
                     /locus_tag="SI621_001606"
     CDS             4789..5526
                     /gene="bamD"
                     /locus_tag="SI621_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEI4064337.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5797..6138
                     /gene="raiA"
                     /locus_tag="SI621_001607"
     CDS             5797..6138
                     /gene="raiA"
                     /locus_tag="SI621_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEI4064338.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6241..6366
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6242..6289
                     /gene="pheL"
                     /locus_tag="SI621_001608"
     CDS             6242..6289
                     /gene="pheL"
                     /locus_tag="SI621_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HEI4064339.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6388..7548
                     /gene="pheA"
                     /locus_tag="SI621_001609"
     CDS             6388..7548
                     /gene="pheA"
                     /locus_tag="SI621_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HEI4064340.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7591..8712)
                     /gene="tyrA"
                     /locus_tag="SI621_001610"
     CDS             complement(7591..8712)
                     /gene="tyrA"
                     /locus_tag="SI621_001610"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HEI4064341.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8723..9793)
                     /gene="aroF"
                     /locus_tag="SI621_001611"
     CDS             complement(8723..9793)
                     /gene="aroF"
                     /locus_tag="SI621_001611"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HEI4064342.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10002..10367
                     /locus_tag="SI621_001612"
     CDS             10002..10367
                     /locus_tag="SI621_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEI4064343.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISA
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10517..11035
                     /locus_tag="SI621_001613"
     CDS             10517..11035
                     /locus_tag="SI621_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEI4064344.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11025..12251
                     /gene="dgcN"
                     /locus_tag="SI621_001614"
     CDS             11025..12251
                     /gene="dgcN"
                     /locus_tag="SI621_001614"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HEI4064345.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGSAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIVQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEVAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12267..12749
                     /locus_tag="SI621_001615"
     CDS             12267..12749
                     /locus_tag="SI621_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEI4064346.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12826..13173)
                     /gene="rplS"
                     /locus_tag="SI621_001616"
     CDS             complement(12826..13173)
                     /gene="rplS"
                     /locus_tag="SI621_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEI4064347.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13215..13982)
                     /gene="trmD"
                     /locus_tag="SI621_001617"
     CDS             complement(13215..13982)
                     /gene="trmD"
                     /locus_tag="SI621_001617"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEI4064348.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKKEHAQQQHKHDGMA"
     gene            complement(14013..14561)
                     /gene="rimM"
                     /locus_tag="SI621_001618"
     CDS             complement(14013..14561)
                     /gene="rimM"
                     /locus_tag="SI621_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEI4064349.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14580..14828)
                     /gene="rpsP"
                     /locus_tag="SI621_001619"
     CDS             complement(14580..14828)
                     /gene="rpsP"
                     /locus_tag="SI621_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEI4064350.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15077..16438)
                     /gene="ffh"
                     /locus_tag="SI621_001620"
     CDS             complement(15077..16438)
                     /gene="ffh"
                     /locus_tag="SI621_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEI4064351.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRYITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16605..17396
                     /locus_tag="SI621_001621"
     CDS             16605..17396
                     /locus_tag="SI621_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEI4064352.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17417..18703
                     /locus_tag="SI621_001622"
     CDS             17417..18703
                     /locus_tag="SI621_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEI4064353.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18757..19350)
                     /gene="grpE"
                     /locus_tag="SI621_001623"
     CDS             complement(18757..19350)
                     /gene="grpE"
                     /locus_tag="SI621_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEI4064354.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19473..20351
                     /gene="nadK"
                     /locus_tag="SI621_001624"
     CDS             19473..20351
                     /gene="nadK"
                     /locus_tag="SI621_001624"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEI4064355.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20437..22098
                     /gene="recN"
                     /locus_tag="SI621_001625"
     CDS             20437..22098
                     /gene="recN"
                     /locus_tag="SI621_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEI4064356.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22247..22588
                     /gene="bamE"
                     /locus_tag="SI621_001626"
     CDS             22247..22588
                     /gene="bamE"
                     /locus_tag="SI621_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEI4064357.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22650..22940)
                     /locus_tag="SI621_001627"
     CDS             complement(22650..22940)
                     /locus_tag="SI621_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEI4064358.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDTVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22930..23367)
                     /gene="ratA"
                     /locus_tag="SI621_001628"
     CDS             complement(22930..23367)
                     /gene="ratA"
                     /locus_tag="SI621_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HEI4064359.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTNNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23538..24020
                     /gene="smpB"
                     /locus_tag="SI621_001629"
     CDS             23538..24020
                     /gene="smpB"
                     /locus_tag="SI621_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEI4064360.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24235..24597
                     /gene="ssrA"
                     /locus_tag="SI621_001630"
     tmRNA           24235..24597
                     /gene="ssrA"
                     /locus_tag="SI621_001630"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24758..28072)
                     /locus_tag="SI621_001631"
     CDS             complement(24758..28072)
                     /locus_tag="SI621_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4064361.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGQLTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGILDVREKGSATGIQQSSQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNAGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIASNLATNMLLENGGNLLVLAGTEARDST
                     VDKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATFTIGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAPATTNGIYNTLTTSELSGSGNFYLHTN
                     IAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGNTGGFVD
                     LGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDPKPDPTPAPTPTPVP
                     EKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVWGTTYNTRNNVTT
                     DAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVGSYSLGG
                     YASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGLGGHIETGMRFTDGN
                     WNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEIEPW
                     LKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSHGA
                     GVESPWNAVAGANWSF"
     gene            complement(28144..28263)
                     /locus_tag="SI621_001632"
     CDS             complement(28144..28263)
                     /locus_tag="SI621_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEI4064362.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(28224..28409)
                     /locus_tag="SI621_001633"
     CDS             complement(28224..28409)
                     /locus_tag="SI621_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064363.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     IAFKKCLFLMRDIIISS"
     gene            28510..28683
                     /locus_tag="SI621_001634"
     CDS             28510..28683
                     /locus_tag="SI621_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064364.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            28685..29029
                     /locus_tag="SI621_001635"
     CDS             28685..29029
                     /locus_tag="SI621_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEI4064365.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            29033..29368
                     /locus_tag="SI621_001636"
     CDS             29033..29368
                     /locus_tag="SI621_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HEI4064366.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(29424..31667)
                     /locus_tag="SI621_001637"
     CDS             complement(29424..31667)
                     /locus_tag="SI621_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4064367.1"
                     /translation="MNGDNSVETRGAYSAGLLSQVNDSGIVENNTRLETTDKTNIVTY
                     GENAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRADLKMNGGSITTNGTNSYGA
                     YANGEKAYINLDYVALETGEHGSYAVAIRQGNIDIKNSSITTKGTKAPIAKIYNGGEL
                     FFSNVTAVSEQDKGISIDASNIDSQAKIALSSVELSSALDSIDVNKTTTDVSILNRSI
                     ITPGNNILVNNTGGGLNIISSDSTLNGATKLVSGTTTLKLSENTIWNMKDDSVVIHLT
                     NSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRLIVEGNTS
                     GSTTVYVKNAGGSGAATLNGIELITVNGDESPADAFRQGDARIAAGAFEYQLKQQGKN
                     WYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGE
                     TRYIDPVTEQERSSRLWLRQVGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSD
                     SWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDAWAQ
                     YSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIW
                     MGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIH
                     NSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNSAASVWGNVGVQLGDNGYNDTAMM
                     VGLKYKF"
BASE COUNT         8067 a         8401 c         7456 g         8169 t
ORIGIN      
        1 acagagtcaa gcattttttg cttttctctg ttgagattct caggagaacc tcgctgaccc
       61 ggcggcgtgc ttgccgttgt tccgtgtcag tggtggcgca ttatagggag ttattccggc
      121 ctgacaagcg aaaaatataa aaactttatc gttcgctcat ttttcaggca aaacatctta
      181 aatatagtct tttccgtcta acttatagac aaaaacgagc cccgaagggc tcgttttatc
      241 atttactgga cggcgacaat ccggtcttca ttaacttcca ggcgaatcac tttacccgga
      301 accaattcac cagacagtat ttgctgtgcc agcgggtttt caatctgctg ctgaattgca
      361 cgtttcagag gacgtgcgcc atagaccgga tcgtaaccgt tctcgctcag cagtttcagc
      421 gcctcgtcag aaatgtggat ttcataacca cgttcttcca gacgtttata cagacgtttc
      481 aactgaatct gcgcaatcga cgcaatgtgc tgttcaccca gcggatggaa gaccaccact
      541 tcatcgatac ggttaatgaa ttccggacgg aagttatggc taaccacacc gagcaccagc
      601 tctttcatgt gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg
      661 ttagaggtca taatgacgac cgtattacgg aagtcgaccg ttctcccctg cccgtcagtc
      721 agacgcccat catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc
      781 acttcatcca gcaggataac ggaatacgga cgacgacgca cagcttcggt caggtagcca
      841 ccttcttcat aaccaacata tcccggaggc gcaccaacca gacgagacac cgaatgtttc
      901 tccataaact cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc
      961 gccagcgcct tacaaagctc agttttcccc acacccgttg ggccgaggaa caggaatgaa
     1021 ccaatcgggc gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc
     1081 gcatcaaccg cttcgttctg accaataacg cgatggtgca gttcttgctc catacgcagc
     1141 agtttttcgc gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc
     1201 acttcagcaa tttcggcatc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg
     1261 agctgcgttg cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca
     1321 gacatccgcg ccaggtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc
     1381 agttccgctt taatggtctg cgtaccagaa agcgatgctt tctctgcttt ccactcttct
     1441 tctaactcgg agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccaggcgt
     1501 tttttactgg cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg
     1561 atacgacgat cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg
     1621 gatgctgctt catcgatcag gtcgatggct ttatcaggca gctgacggtc agcaatgtag
     1681 cgatgagaca acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc
     1741 aattcgtaac gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg
     1801 gcaacaaaca ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga
     1861 tattcgtcaa gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc
     1921 agcatgtttc cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat
     1981 tcgtcgataa acaggatgac gttgccttcc tgtttagcaa gatcgttaag tacgcctttt
     2041 aaacgttctt caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc
     2101 gccagtacgc ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc
     2161 agaccttcaa cgatggcagt tttaccgacg ccgggttcac caatcagtac tgggttattt
     2221 ttagtacgac gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc
     2281 ggatcgagtt tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc
     2341 tgacgttggt cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc
     2401 gcttgagtaa tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg
     2461 cgagactcaa gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt
     2521 tgcgccagct tgtcgcaaag attaagaacg cgcaccagat cttgtgatgg ctggacatca
     2581 ccaccagtac cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac
     2641 tggccagcat ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc
     2701 agcagggcgc tcattaaatg aagtggttcg atgaattggt tgtcgtgccc gagtgcaagt
     2761 gattgggcat cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact
     2821 cctcccataa cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc
     2881 aaggttattg accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct
     2941 ttaggttata tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa
     3001 gagaagaaag tctcattttc cgtatatgta caacggttgc cgccgaaaat ttgttcgaca
     3061 ccgacgttcg ccagacgctg ccgggcaagg agagaaatat ccgccagata cttatcacca
     3121 tgctgaatga aagctgtact cgctttggcg tctactgcca taaacgcctc gcgaacctcc
     3181 gcccccactt caaacgcgcg tggaccaatt gccggcccta accaggcgag aatattttcc
     3241 ggattatcag caaaacagga aaccgtctcc tccaacacgc cagcgcacag tccacgccag
     3301 ccagcatgag cggcggcgac ttccgttccc gctcgattgc aaaacagcac ggggaggcag
     3361 tcggcagtca tcactgcgca aaccgtgccc ggcgtgttgc tataagaggc atctgctcgt
     3421 tttgaggcat aaggttcgcc agtaagctta agcacatctc tgccgtgtac ctgctcaagc
     3481 cagaccggtt ttgaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg
     3541 tgatccgggt tatcgccaca atgagcaccg aggttgagcg agtcatacgg gggcaagctc
     3601 acgccgccga tacgagtaga gctacaggcc gcaacacctt ttggcagggg ccactgcggg
     3661 acaatcagct tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca
     3721 tcacctcaat cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatgc
     3781 cggagattgg gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaacttac
     3841 gcagcgtgga gataaacgct tccgaagcac cttttggcgg acgcggacgg ccaccataaa
     3901 ccggatcgcc caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac
     3961 gtccagtttc cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat
     4021 agtgagtgac cgccggtttg cccatgggat gcaccgccat gtgagtacgt ttggtcgggt
     4081 ggcggctgat tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt
     4141 catactcacg agtaatttca cgccgttgca aagattcgac taaacgcgtc tgagccggaa
     4201 cggtttttgc cacaaccatc aggccggtgg tgtctttatc cagacgatgg acgatgcccg
     4261 cacgcggtac atcggcaatg ggcggatagt aatgaagtaa cgcattcagt accgtaccat
     4321 ccgggttacc cgcgccagga tgtaccacca ggtcgcgtgg tttattaatg acgatgatat
     4381 cttcatcttc atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt
     4441 caatctcagc gttgatggcc acctgctcgc cacccaatac tttttctttc ggcttatcac
     4501 aaactttgcc gttaaccagc acgcgctggt cgaggatcca ttcttttatc cgcgaacgtg
     4561 aataatccgg gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg
     4621 acaccgttgc agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg
     4681 gggttttacg gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc
     4741 aggaacagag aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg
     4801 aaatatctgg tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag
     4861 gaagaagtac ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag
     4921 gacggtaact ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt
     4981 ggtccgtatt cgcagcaggt gcagctggat ctcatctatg cctactataa aaacgccgat
     5041 ttgccgttag cacaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat
     5101 atcgattatg tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg
     5161 caagggttct ttggcgtcga tcgtagcgat cgcgatcctc aacatgcacg agctgcgttt
     5221 agtgactttt ccaaactggt gcgcggctat ccgaacagtc agtacaccac cgatgccacc
     5281 aaacgtctgg tattcctgaa agatcgtctg gcgaaatatg aatactccgt tgccgagtat
     5341 tatacagaac gtggtgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac
     5401 tacccggata cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag
     5461 atgcagatga atgcgcaagc tgaaaaagta gcgaaaatca tcgctgcaaa cagcagcaat
     5521 acataacaaa aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt
     5581 tagttgtgaa actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga
     5641 gatattcctc aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga
     5701 caaaaagtga caaaattatg agatttccat cacacatttt gacatcagga acggtatgct
     5761 gaattcacca agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa
     5821 caaatggaaa ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa
     5881 aaatggcaaa cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt
     5941 gttgctgacg ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa
     6001 gatatgtaca ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg
     6061 cagcacaaag gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa
     6121 gaagttgaag aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt
     6181 tttttgttga cagcgtgaaa acagtacggg tactgtactg aagtcactta aggaaacaaa
     6241 catgaaacac acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt
     6301 ttcgtcgtgt gaaacagaat gcgaagacga acaataaggc ctcccaaatc ggggggcctt
     6361 ttttattgat aacaaaaagg caacactatg acatcggaaa atccattact ggcgctgcga
     6421 gagaaaatca gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg
     6481 gccgtcgagg tgggaaaagc caaattgctc tcgcatcgcc cggtacgtga tattgatcgt
     6541 gaacgcgatt tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat
     6601 tacattactc gcctgttcca gctcattatt gaagattccg tattaactca gcaggcgttg
     6661 ctccagcaac atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc
     6721 aaaggttctt attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc
     6781 attgaaagtg gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc
     6841 gactatgccg tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat
     6901 ctgctgcaac ataccagttt gtcgattgtt ggcgagatga cgttaactat cgaccattgt
     6961 ttgttggtct ccggcactac tgatttatcc accatcaata cggtctacag ccatccgcag
     7021 ccattccagc aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc
     7081 gaaagtacgt ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg
     7141 ttgggaagcg aagctggcgg cactttgtac ggtttgcagg tactggagcg tattgaagcg
     7201 aatcagcgac aaaacttcac ccgatttgtg gtgttggcac gtaaagccat taacgtgtct
     7261 gaccaggttc cagcgaaaac gacgttgtta atggcgaccg ggcaacaagc tggtgcgctg
     7321 gttgaagcgt tgctggtact gcgcaaccac aatctgatta tgacccgtct ggagtcacgc
     7381 ccgattcacg gtaatccgtg ggaagagatg ttttatctgg atattcaggc caatcttgaa
     7441 tcagcggaaa tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta
     7501 ttgggctgtt acccaagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc
     7561 ggatgatgtg aatcatccgg cgctggatta ttactggcga ttgtcattcg cctgacgcaa
     7621 taacacgcgg ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt
     7681 gcggaaactg tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc
     7741 gaaacgctta tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc
     7801 ggcataaagc tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta
     7861 aatcggcgaa gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag
     7921 cccgtaagca aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg
     7981 ctcgacggcg ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca
     8041 ttggtatgct tccggcttac gtccatcaca ccagaccaca acttgctttg ccaggctacc
     8101 gctgtccgga ccgaacattg ggtgtagacc cagtaccggg ccatcgtgcg ccgccagcat
     8161 ggcctgtaat ggtccatttt tcaccgatgc cagatcaacc agaatacaat ctttcggtaa
     8221 aggcggtaat ttgccgataa cttgctcagt aacgtggatt ggcacactaa caatcaccat
     8281 tccggcatcg gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac
     8341 ctgataaccc gatagtgtca gcatcttctc gaacaggcgt cccatctgac cgccaccgcc
     8401 gacgataacc actggacgca gtgacggaca aagtgtttta aatcctttgt cgttttcact
     8461 ggagtaagat tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc
     8521 cagagcttcc gcctctgcac gacgcgaggc caacatagat gcctcgcgct ccggaacata
     8581 aataggcagt ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt
     8641 cgctaataaa ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa
     8701 ctcagcaacc ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct
     8761 gatgaatttc acgcagcaag gcatcggtca tttcccaact aatgcaggca tcggttacgg
     8821 atacaccgta tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat
     8881 tactttcgat catcagacca ataatcgagc gattgccatc tttgatctga gcaaccacgg
     8941 actctgccac cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta
     9001 ccatcagaga cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg
     9061 cagggctata gttcggcgct ttaccaccgc gcaggatcac atgcccgtcc ggattcccct
     9121 gagtttgtag caacgccacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg
     9181 cggcggcgcg catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaaac
     9241 caaccggcat ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag
     9301 caccaattgc tgaccagcta aacagatcgc ccaggtattg cgggctattt ggatctaacg
     9361 cttccgtcgc cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct
     9421 gcagcccggc ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc
     9481 agccgacagt ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc
     9541 tgacctctgc ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat
     9601 gaatggaaca aggaccacat actaccaaca gacgaggatc gcgcccggcg ataatatctg
     9661 aaatggtttt acgcgagtca gcaatctggg cttcttgttg caggctcaat ggaaaagcgg
     9721 ccttcagttg ttccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg
     9781 cgtctttttg catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga
     9841 ggatgacgta acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttct
     9901 ttacagaaac aatttactcg cgcttttgat gcttatttcg tacaaataaa tttacacctc
     9961 ctttaaaaac aatccgctat gctttgaaaa aggagaaaga aatgatgaaa aagtttatcg
    10021 cccccttgtt ggctttactg gttagcggat gtcagattga tccttatact cacgcgccaa
    10081 ccttgaccag caccgactgg tatgatgtcg gtatggaaga tgcgatatcg gccagcgcca
    10141 taaaagatga cgatgcattt agcgattcac aggcggatcg cggtctatac cttaaaggat
    10201 atgccgaagg acaaaagaaa acttgccaga ccgattttac ttatgcccgg ggcctttccg
    10261 gtaaaagctt tcctgccagc tgcaacaatg ttgaaaatgc cagtcaactg catgaagtat
    10321 ggcaaaaagg ggctgatgaa aatgccagca caatacgatt aaattaatta accccccaca
    10381 aataaattaa acatgagatt ctactcattg tgttttattt ctcacattga tgacgaccgc
    10441 atgaaagaat gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga
    10501 tggtgaggat ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct
    10561 gacgggagca ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat
    10621 ggtttccggt attgtcagtt acacccgctg gcctgcgcta tcagggccac caaagttatg
    10681 tatcttttca tcttcacatt ttagtaacgc cctacaagag aatggtacga catctttgcc
    10741 ttatctgcct gtcatcattc acactcaaca agaggcgatg atttcaggct gcaatggttt
    10801 ttattttggc aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa
    10861 ggcgttatta ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct
    10921 gatcatccac aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg
    10981 tgtaaaggtc aacccggatg tcttgatgct cgcacggaag aaaaatgatg gataacgata
    11041 attctcttaa taagcgcccc acgtttaaaa gagcattacg caacatcagt atgaccagca
    11101 tatttatcac tatgatgctg atctggttgc tgctttccgt gacctcggtg ctgaccctga
    11161 aacagtacgc gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag
    11221 cagcggtcgt ttttgccgat ggctctgcag caactgaaac actggcagcg ctggggcagc
    11281 aaggccaatt ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc
    11341 attacacccg taaggatcca ggcgatactt tcagtaattt cataagccac tggctcttcc
    11401 ctgcccccat cgttcagccg attcgtcaca atggtgaaac cattggcgaa gttcgcttaa
    11461 ccgctcgcga cagttcaatc agccatttca tctggttttc gctcgctgtc ctgaccgcct
    11521 gcattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggtctgg
    11581 ttgaagcgtt aaaaaacatc accgatgtcg tacatgatgt gcgttccaac cgcaattttt
    11641 cccgacgagt ttcggaagaa cgtatcgcag agtttcaccg cttcgctctc gacttcaaca
    11701 gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc
    11761 tgcgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg
    11821 gtatcaatac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg
    11881 atggcgataa tttcaaatat atcaatgata cctggggtca tgcgacgggc gatcgtgtct
    11941 tgattgaggt agcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc
    12001 tgggcggcga tgaattcgcg atggtgctct atgatgtaca gtcggaatct gaagtgcagc
    12061 agatatgctc agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc
    12121 agaccaccat gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa
    12181 aattacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc
    12241 tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca
    12301 cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg
    12361 ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag
    12421 atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc
    12481 aaaccatggc agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca
    12541 ccgataacta tggtgaagat agttacaacg aaggcttatc attgaaacgg gcgaatgtcg
    12601 tggccgatgc atgggctatg ggtggacaaa ttccgcgcag caatctcacc acacagggtt
    12661 taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc
    12721 gcgtcgcagt ggtgattact accccttaaa acacgagcaa acagataaaa aaagggccag
    12781 ccaattggcc agcccttctt aacaggatgt cgcttaagcg aaaccttagt taagacgctc
    12841 tttgatacga gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac
    12901 agcaccacga cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg
    12961 ctcaacgcct tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt
    13021 acgaatagcg ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac
    13081 ccatactttc acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg
    13141 ctcttgttca agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact
    13201 gatatctcgg gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccttt
    13261 ttgaactccg ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca
    13321 ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca
    13381 tggttgcccg acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta
    13441 tagtgcgggc aatccagcaa tccttcagca aaggaatctt ccgttgctga ggcttcatgt
    13501 cccagtactc ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca
    13561 ccaccactga gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg
    13621 cgctcatcta taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt
    13681 tcgctgacgc ccgcttgatc aagcttgcgc ccctgtggtg aaagataaat cacctttgcg
    13741 ccttcacccg ccgcggcttt tgctgcatga atggcgtccc gcaaaggttg caccatcatt
    13801 aacatccccg gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg
    13861 aagtcgcgag gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt
    13921 accccgtaat cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac
    13981 atagcgccgt cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg
    14041 attgaacgag tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga
    14101 cgttccttga taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat
    14161 ccggtttcca tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg
    14221 cagcccatca ggtctttcca gtagtagtcc ccctcttcaa gctgaggcag ctgcgatgaa
    14281 tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc
    14341 agcttgatga tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac
    14401 tgacccgcct tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg
    14461 gaagaaaaca ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg
    14521 atgggatcaa caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat
    14581 taagctgctt tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc
    14641 tggccaaccc agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta
    14701 gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg
    14761 tcagcgacaa caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata
    14821 gttaccataa catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc
    14881 ggtgtcatat taaaagcccg aaaattttac tcatttttgc ggaaattgca atcaacagtt
    14941 gctaactctg ctgtaaaagg ccgtcggcgg tgcagccagt ttggtgccgg agtgcgcgca
    15001 gtcaccggag cgtacacgca gtacgtgagg atgacgagca caccccggtg ccaaaatgac
    15061 aaacaagcca ggccgattag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
    15121 tcatcatctt cgccattccg cccttcttca ttttcttcat catgcgctgc atgtcgtcga
    15181 actgcttcag aaggcggtta acgtcctgta cctgcatccc gcaaccggcg gcgatacgac
    15241 gcttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
    15301 tgatggcttc catacgcacc agcactttat cgtccatctg cgacttgacg ttatccggga
    15361 tctgccccat gcccggcagc ttgcccatca ggctagccat gccgcccata tttttcatct
    15421 ggcgcagctg ctcaagaaag tcgttgagat cgaagccgtc gccttttttc agcttgctgg
    15481 ctaatttctc tgcctgcgcg cggtcaactt tgctttcaat atcttcgatc agtgacagta
    15541 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
    15601 cagttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtaa cgaatagaga
    15661 gcgccgcacc accgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
    15721 acgcttcatt aaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
    15781 caaacagggt ttcaaccgga ttaatcgacg catggacttg tttgatctcg tccatcatcg
    15841 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
    15901 tggcttcttt cagcgccgcg ttaacaatat caaccggctt ctgaccaaca tcagaaggga
    15961 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
    16021 aaacgtcggc ggaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
    16081 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc tgccatcagc acgaccgcag
    16141 gcggttgcgc agccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
    16201 tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
    16261 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acgtccgcct
    16321 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gtgaggcgtc
    16381 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
    16441 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
    16501 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
    16561 ctatactgct tctctttctt attgctcaaa ctgtcgacat tactatgccc gtttttgctc
    16621 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
    16681 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
    16741 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
    16801 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
    16861 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
    16921 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
    16981 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
    17041 tgcaactggc gtggattgat taccaactga agaacaagaa actggcgttt aaccaggaaa
    17101 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
    17161 tgctaacgct cacgctttgc actggcctgt tctacatgca caacttattt agcatggaaa
    17221 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
    17281 gacattatca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggtgcag
    17341 tcattctgac actggcctac ttcggcagtc gaattgtcca gcagttaatc agctaaaccc
    17401 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17461 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17521 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17581 gctgcgtaag ccagaccgcc tgattagcct ggtgttaatc ggcaataacc tggtcaatat
    17641 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17701 aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat tgccgaaaac
    17761 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
    17821 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    17881 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    17941 cactatcgtg cacgaatcgc gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
    18001 ggtgctcgat ctggaaaaga tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
    18061 cggtattgat atcaacgatg actggaaatc gattctgcgc caactatccc actcacctca
    18121 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
    18181 tgaagcctgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
    18241 cgcggacgag atctattttg tcccggaagg tacgcccctc agcacgcagt tggtgaagtt
    18301 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaagggct
    18361 ggtgacggtt gaagatattc tggaagagat agttggtgat ttcaccacgt cgatgtcgcc
    18421 aacacttgcc gaagaagtca ctccgcaaaa cgacggttcg gtgattatcg atggcaccgc
    18481 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
    18541 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
    18601 tattggcgag tacgatatcg atattctcga cgtacaggac aatatgatta agcaggtaaa
    18661 agtttttcct gtaaaaccgc tgcgcgagag tgtggcggag taaaaaaacg gcccggcatt
    18721 cgcatgctgg gccgttattt attacgaaaa cagaaattaa gcttttgcct tcgctacagt
    18781 caccatcgcc gcacgaattg tacgaccgtt cagcgtataa cccttctgca taatgcccag
    18841 tacgttacct ggcgcaacgt catcagattc caccattgcg atggcctgat gcacattcgg
    18901 gtccagcggg acgttagttt cggcgatcac ttcaacgcca aacttacgca caacatccag
    18961 catcgacttc agcgtcagct caataccttc aaccatcgcg gacatatccg ggttagcttt
    19021 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
    19081 tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac gacgcaggtt
    19141 ttccatttcg gcttttacgc gcaaaatgcc gtcacgttca cgagtctggg cttcagccag
    19201 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcgtc
    19261 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
    19321 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
    19381 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
    19441 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
    19501 ttgtgggaca cccacggcac cccactgcac tgacaacaca tgaaatgctc taccgctggc
    19561 tgtgcacaaa aggttacgag gtcatcgttg agcagcaaat cgctcacgaa ctgcaactga
    19621 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc gcggtagtcg
    19681 ttggtggcga cggtaatatg ctgggcgcgg cgcgcacact cgcccgttac gatattaaag
    19741 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
    19801 agcaacaatt agccgatgtg ctggaaggcc actacatcag cgagaaacgt tttttgctgg
    19861 aagcgcaagt ttgtcagcaa gattgccaga aacgcatcag cacggcgatt aacgaagtgg
    19921 tacttcatcc tggcaaagtg gcgcatatga ttgagttcga agtgtatatc gacgagatct
    19981 ttgcgttttc tcagcgttct gatggcctga ttatttcgac gccaacaggc tccaccgcct
    20041 attccctctc tgcgggcggt cctattctga caccctctct ggatgcaatt accctggtgc
    20101 ccatgttccc gcatacgttg tcagcgcgac cgctggtgat aaacagcagc agcacgatcc
    20161 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
    20221 tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
    20281 ttcatccgaa agattacagt tatttcaata cattaagcac caagctcggc tggtcaaaaa
    20341 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac
    20401 aataacagta atggtttttc atacaggaaa acaactatgt tggcacaact gaccatcagc
    20461 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
    20521 ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggcctttg tctcggtggt
    20581 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgtg ccgacctgtg cgcccgtttt
    20641 tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct tgaagacggg
    20701 catgaatgtt tgcttcgtcg cgtaatcagc agcgatggtc gctcccgtgg ttttatcaac
    20761 ggtacagcgg ttcctctttc acaactgcgc gaactgggtc agttgctgat tcagatccat
    20821 ggtcagcacg ctcatcagtt actcaccaaa cccgagcacc aaaaattcct gcttgatggc
    20881 tatgccaatg aaacctctct actccaggaa atgaccgcac gttatcagtt gtggcatcaa
    20941 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc ccgtgcagaa
    21001 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgag
    21061 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac taccagccag
    21121 aatgcattgg cagtaatggc cgacggtgaa gacgcaaacc tgcaaagtca gctttacacg
    21181 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
    21241 atgctggaag aagctaccat ccagattgct gaagccagcg atgaactgcg ccactactgc
    21301 gatcgtctgg acctcgatcc taaccgactg tttgaacttg aacagcgcat ctcaaaacag
    21361 atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat tgccacagta ttaccagtcg
    21421 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gctcgcgctg
    21481 gcggtaacga aacatcatca gcaggcactg gaaacggcgc gcgcgttaca ccagcaacgc
    21541 cagcactatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc actctctatg
    21601 ccacatgggc agtttacgat tgatgttaaa tttgacgagc atcacctggg cgctgacggt
    21661 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
    21721 aaagttgcat ccggtggtga attgtcccgc atcgcattgg caattcaggt catcacggcg
    21781 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggccca
    21841 acagcggcag ttgttggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
    21901 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    21961 accgatggtg cgatgacaga aacgcatatg caatccctgg ataaaaaagc gcggttacaa
    22021 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc gaatgcgaaa
    22081 gaactgcttg cggcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    22141 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    22201 cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    22261 gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg
    22321 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    22381 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    22441 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    22501 tgtaacgcag caaacgctga cgctgacctt taacagtagc ggagtgttga ccaacattga
    22561 taacaaaccc gcgctgagtg gtaactaatc ataaagttgc cctcaaagac gttaaaaaag
    22621 gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc
    22681 ctgcgcagct ctttcggatc ggcaatgaga ggacgataaa tctccacccg atcgccatca
    22741 tgcacggtat cgctgagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa
    22801 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    22861 ccctcctgca gcgtcacgcg ctgcaggtac tgcttctcag gtagcgcata agccacctca
    22921 acggcaattt tacctggcac tgtaaacctc tttcgcccga accgtaaaag cctggaccat
    22981 attagccgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa
    23041 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    23101 gccaccgatc aatttcttga acggcccatc caccagattc ataagaatac tttggttatt
    23161 ggtcaactgg ttgcgggtag taaacgtttt gctgatcccg gccttagaga catctaccgc
    23221 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa
    23281 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    23341 cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc caacaaataa
    23401 tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg
    23461 ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc
    23521 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    23581 cgcttaacaa gcgcgcccgg cacgaatact ttatcgaaga agagttcgaa gcgggacttg
    23641 ccctgcaagg ctgggaggtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct
    23701 acgtccttct acgtgatgga gaagcatttc tgtttggcgc taacatcacg ccaatggctg
    23761 tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    23821 gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    23881 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaagaaac
    23941 agcacgataa acgttcagat atcaaagagc gcgaatggca ggtggataaa gcgcgtatca
    24001 tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcctc
    24061 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca
    24121 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    24181 ccattgaggc tggtcatggc gctcataaat ctggtatact tgccgttaca cattggggct
    24241 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    24301 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    24361 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    24421 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    24481 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    24541 gtagtaccga ggacgtagga atttcggacg cgggttcaac tcccgccagc tccaccaatc
    24601 atgattggac ggtgcaagga caacaccaac aaaaacagga agttagaagt ctcagcaaga
    24661 caccgaccag acggtgagga gacataaaag gatacgcaaa ggagccgcgg ctcttggtga
    24721 tatgaaagcc cacagatgtg ggctttttcg ttgatggtca gaacgaccag ttcgcaccag
    24781 ccaccgcgtt ccacggggat tccacaccgg caccatggct ataccccacc ccgagatgtc
    24841 cgcttaacgt actgctgaat gaggctttaa tacctgcctg gtatattcca cgtctgcccg
    24901 acaaatcatt gacgaaatta ccgtcattat tcaccttcac ccggttatca tcgacaaatt
    24961 ctttgcgcac agccgccttc agccacggct caatttccat accgttcccc agacgcatgt
    25021 tgtaactcag cgttgcgccc agttcacgat atatactgcg ggtatcgact gatttcgatt
    25081 ccatgccatt ggataaatga tattcggggt tatcagcggt gaaccccgtt aacgatgcat
    25141 acggcgtcag gttccagtta ccatccgtaa atcgcatccc ggtttcaatg tgaccgccca
    25201 gcccgttgct gcgataactg ccgttggcgg ctccaccgct gctcatttta ccggctacgt
    25261 tactttcaaa gcggttcagc ttcacgatac cgtccagata gaaaccactt tcatgttccc
    25321 agctggcata gccccccaga gaataactgc ccacactgcc atgtcctcca cgatcaaaac
    25381 caatatgtga atgggaataa cccataaaag cgcccagcgt cgcaatcccc tcaggaatat
    25441 cattacggct atcgatcccc accgtcattc cggtcagcgt ctgctcaaac ccggcacccg
    25501 catcggtggt gacattatta cgggtgttat acgtcgtccc ccagacatta ttgttgtgtg
    25561 gactcgcttt cattatgttc aaccgctcgc gaatactgtt tagctcagca tcaaagacca
    25621 acggtaatgt tgctgccata ttgagtacgg ctgctgtaga aggcgtgatg cgtttctccg
    25681 ggacgggtgt cggcgttgga gcgggagtcg ggtctggttt cggatccggt tttgggtctg
    25741 gttttggatc cggtttcgga tttgggttgg ggtccgggtt gggtttgaca tcattggtca
    25801 ggttccagtt gctgttgccg tcgcttttca ggacatactc ataggtccca agatcaacga
    25861 aaccaccggt attgcccagc gtaaacgacg catcccctcc ccctgttttc accagcgtca
    25921 tcgcatcgtc agactgaggg ctgacgccgg tatcctgaac aaagatttta aaattaccag
    25981 tggcattgtt gttgacgacc agttgatcgc cccgggagcc tgcaatgttg gtatgcaggt
    26041 agaaattacc gctgccggaa agttcactgg tggtcagcgt attgtagata ccgttagttg
    26101 tggcaggcgc tgataaataa acatcaccgt cgttgagtag caaactgttt actctatatt
    26161 cgcagttgct ggtgcctgca caggaattat tgctgttaag ccagacactg ccccggctgg
    26221 cagccgtcag gtcggcaagc gtcgtatcaa cgccattgcc gatagtgaat gtcgcgctat
    26281 cggtaatccg gatcgcccct tcgagtttaa ctggcgtaac attatcccgt ggcgtcatta
    26341 acgacaggct tcctgtcgcg ccactgaccg atgcatccgc cagcgtacct gcgtagacga
    26401 ttgctgtccc gccagcaacc tggagatctt ctgcccgggc cagagcctga aactcatctt
    26461 ttgaccgccc aagggtatat tgcccaccag agttaacctt tgtggcggag tcctgaccca
    26521 ggttttgcat tgcaccaccc ttgtcaaccg tggagtcacg ggcttcggta cctgctaata
    26581 ccaataaatt accgccattt tccagcaaca tattggtcgc taaattgctg gcaatggaaa
    26641 aggtgccgta ctggtgcgta ccgctgattt caataccgtt agccgtgctc gtctggagag
    26701 cggcaccgct gttctggacg atatctgtcg ctttgccatt atcgttgact gtcagcgttc
    26761 cgccttcatt gatcgttgtt tttattgcct ctccgttagc tgaaactgtt tgtattccgc
    26821 cgtcgttaat tgttgtctca tttgccacac cctcgacgat ttgttcaccg ccagtgagcg
    26881 tcgtgcctgt cgcagtggct tttgttttga cgatctcccg accgcccgca ttgacctgtg
    26941 ttttggcgga agtggtgtct gactccacgg ttaacacgcc gccatttgtc agcaggatat
    27001 tgttcgccgc accctgctcg atgctgaacg cgacgccatc agcgcgtgtc cctgtgaccc
    27061 gcgtcgccct ggtggttgca accaacgcgc cctgactact ctgctgtatc cccgttgcgc
    27121 tgcctttctc ccgcacatcg agtatgccgc cattgttaag tctggtggta tatgctacgg
    27181 cctcctcgtg gacaatcagc ttaccgccag catcaacgat ggtatctacc gccgaggttt
    27241 gagctacgac cgtcagttgg ccggtatttt ccagcacaac atgctcagcc ctgcctccgg
    27301 taatagtgaa gtgagatgac ctctggtatc catcaatatc agtccccgcg cccgtgttgg
    27361 caactaaagc actccccgtg tccaggtaaa ccccatgtgc gataccgccg gtatcgacaa
    27421 tcagcgtgcc tccggcacga atattgctgc cagttgccgt acctcctttc tcaacaacct
    27481 gcttgctccc ggaagagata tttgttgtgt cagctttccc gccgctcttg atattttgcg
    27541 tgccactgtt gatattagtt cccgtagcaa taccatgatt ataaatattc tgtgtaccgc
    27601 cattaattat ggtattagtc gcgttcccgg caacatccat ggccccgccg ttatctattc
    27661 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatcg ttaattatcg
    27721 tccctgtaga aattcctccc tcactaattg tctggctgcc accgttaatg actgtattat
    27781 tagcctgccc ctgataagag ccatgaccag atacctcttg atgtccacca gtatcaataa
    27841 cacttccatt agaaatcccg ccatatacct cctgattgcc gtgattgatg atatgagcat
    27901 tatttgttgt acctcgttca tctactcttt ggctgccgtc tacagtctca tcatttacaa
    27961 cgccaataac attagaagtg aaggccgtca tcccaggcgg ggcatatatc atggcagcta
    28021 tcaataagga aagtactgag cggtgaaaat aatagcgact gattctgttc ataaatttca
    28081 tcctctgaaa aatgaatact gagtactgag tagcgtctaa gtaacctaag ctcggctgca
    28141 acatcagccc gcaggcacca gaccagggga ttcatcctga agagacagcg caggtgtatt
    28201 gtgttcaccg ctcaccaaag acatcatgat gaaatgatga tatcccgcat aagaaagagg
    28261 cattttttaa acgcaatgcg ctgaagtgta gttggataaa aaatcactct tttcagataa
    28321 tactgtcgta cgcttatctt tcgacaatga ggccgtcggc aaaataaaat gactaatgta
    28381 atcgttgctg atgaatatct tctgctcaca taaaaatcac acaataatat tgataaatca
    28441 gattatctta cctttacaac attgcgttaa cagtcaatga cacttcttga ataaaaatat
    28501 tgtcaatcaa tgagttattc ctcatttaag attattctca ttcacgtaaa aaacataatt
    28561 cctatcatca cagccacgct cattttgaat tatctgaata atagcgagcg ttctttagtt
    28621 aagcagatct taattgaaga cgaaattatt gtgtgtgcaa cttacttaat accggacatc
    28681 taagttgaag aaaaaaatac ttgccttcgg gcttatctca gcattgttct gctccacgcc
    28741 agcgatggcg gatatgaacc gcaccacgaa aggtgcattg ctaggcgcag gcgttggttt
    28801 acttactggc aatggtgtta atggcgtact gaaaggtgca gctgttggcg ctggtgttgg
    28861 tgcagtaaca gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa aagtgggtgc
    28921 cgccgttggt gctgtcactg gcgttctgac cggcaatggc ctcgaagggg cgattaaagg
    28981 tgccgtgatt ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag gaatgatgat
    29041 gaaaaaattt gctttactgg cgggtctgtt tgttttcgca ccaatgacct gggcgcagga
    29101 ctacaatatt aaaaacggtt tgccgtcaga aacctatatt acttgcgcag aagccaacga
    29161 aatggcaaaa actgacagcg cacaggttgc agaaattgtt gcggtaatgg gtaatgccag
    29221 cgttgccagc cgtgatttaa aaattgagca atctccggaa ctaagcgcca aagtggtaga
    29281 aaaactgaat caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg caattgatga
    29341 cactatgcgt gctatcggca aaaaataata tttaatatgt taccgcgtcg atgaattcga
    29401 cgcggtattt tagctaacgg ggatcagaac ttatatttca ggcccaccat cattgcggtg
    29461 tcattgtagc cattatcacc cagctgcacg cccacattac cccagacgct ggccgccgaa
    29521 ttcagattgc catttacgcc aagtttaatt tcccccagat tacgcgcccc gtcttgttca
    29581 actttcacac cgttcattga gacagcaaaa tctttactgt tatggatcca gttcatttca
    29641 acaaaaggtt caaagtcgtc accgtgcgga ccgctgtttt tctcctgagt acgaataaag
    29701 gtgcggaagc cgagtcgggt ctggatgttg ttatttgcgt cattctcaat acgtgagcca
    29761 ttggcttccg tgtgcgcatt atgatcgacg cccatccaga tagcctgtgc ctgtggctgg
    29821 aagatccacg tatatttcgc cgcttccaga ccaaaggatt tattcagggc aaaaccgtaa
    29881 cccgcttcca gcgagacggt tgcacctttc gcgctatagg attcataggc taactcatcc
    29941 cctttcaccg agtttttaaa ccagctatat tgcgcccagg cgtcaatgta tgcgcctttt
    30001 ttactgatgt catcggcaaa ccaggtggca tacagccctg cgctatagcc tctgacactc
    30061 cctttcgaac gataatccga cacgctggaa tgagttgagt tgtagtcgcg ggcataacca
    30121 gccatcactc ccaaacgcca actgtcactg tcggtaaaac caccggttaa cagatcggcc
    30181 cctaactgcg agacgtagcg atgcgaggtc gttctcaact gtccgttgct gtcacgccag
    30241 gcattatgcc cgccaacttg acgtagccaa agccggcttg aacgctcctg ttcagttaca
    30301 ggatcgatgt aacgcgtttc acctgcacga tcgttcagac gcataacaaa aagagtgtta
    30361 gcggctgcca ggttggcgac gtaacttcca gcttcggggc gcaggacggg ggtaggcgtt
    30421 tctgttgatt ctgaatttcc ctctgagctg ttatcctcct cattcactga ctgataactt
    30481 gtcaaatacc agtttttgcc ttgttgtttt agttgatatt cgaaagctcc agctgcaatc
    30541 cttgcatcac cttgtctgaa ggcatctgct ggagattcat cgccattcac agttatgagt
    30601 tcgatcccat ttagcgtggc cgcgccgctt cctccagcat ttttcacata gacggtagtt
    30661 gaccccgaag tattaccctc aacaataagt ctgtcagtag ccgatttatc atcacctaat
    30721 acggtgcgga tattaagctg accattatta ccgacataat taccttttac ggttaacgtt
    30781 tttccctggg taaatgtttg accatcatca tacgaaagat tgataatact gtctgaatta
    30841 gtcagatgga taacaacgga gtcatctttc atattccaga ttgtgttttc tgaaagcttc
    30901 agtgtagttg tgccgctaac gagtttagtc gctccattta gagtagagtc ggacgaaatt
    30961 atgtttaagc cacctccagt attattaacc agaatattat taccaggcgt gataatactt
    31021 cgattaagga tgcttacatc cgttgtagtt ttgttaacat caatactatc caaagcactt
    31081 gaaagttcga cacttgatag tgctattttg gcttgagaat cgatatttga tgcatcaatt
    31141 gatattcctt tatcttgttc tgataccgcg gtgacattgg aaaaaaataa ctctccacca
    31201 ttgtatattt ttgcaatggg ggctttagtg ccttttgttg taatagaact attttttata
    31261 tcaatgttac cttgtcgaat agcaactgca taacttccat gctccccggt ttcaagtgcc
    31321 acataatcta aattaatata tgctttttcc ccattagcat aagcaccata gctattagtg
    31381 ccattagttg ttatggaacc accattcatt tttaaatcag cacggctaat aacttcgtaa
    31441 ctgttagaat cactaacttc atcatctaca gcatcgacac atgttcgaga ctctccagga
    31501 cttgaacatg caagaacacc tactgcattt tccccatagg taacaatgtt cgttttatct
    31561 gtggtttcaa gacgggtgtt attttccaca atcccagaat cattaacctg acttaaaagt
    31621 cccgctgaat atgcccccct cgtctcgact gaattatcac cattcataac gatggtgcca
    31681 ccaaattcac tgactactgc gctggctgtt atccctccat taacatcttg gttatttcct
    31741 ttgtcagata cgcttccaga ggtcttaata tcgctatcgc ttatatataa tactttgtta
    31801 tgactatcaa cagaaatacc ataagatcca tcaccactag tcatcacact ggcattattt
    31861 atatatccag ttacaccgtc atccactttc acagcatggc tttcgtcttt agtggttaga
    31921 tactctccat tacttatcga attattatct ttgatttcaa tcggcgattc ttcatcagtt
    31981 atataggaaa gtatactata atcatccaac aattgtgttg catatgcatt acccaccgcc
    32041 aaagcaataa gtcctaaact aaacccgggg agttttttca gtttcataga tta
//
LOCUS       DARNVM010000039        31941 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.039, whole genome shotgun sequence.
ACCESSION   DARNVM010000039 DARNVM010000000
VERSION     DARNVM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.039"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..3124)
                     /gene="acrB"
                     /locus_tag="SI621_001638"
     CDS             complement(<1..3124)
                     /gene="acrB"
                     /locus_tag="SI621_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HEI4064368.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIE"
     gene            complement(3147..4340)
                     /gene="acrA"
                     /locus_tag="SI621_001639"
     CDS             complement(3147..4340)
                     /gene="acrA"
                     /locus_tag="SI621_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HEI4064369.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4482..5129
                     /gene="acrR"
                     /locus_tag="SI621_001640"
     CDS             4482..5129
                     /gene="acrR"
                     /locus_tag="SI621_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HEI4064370.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5257..8619
                     /gene="mscK"
                     /locus_tag="SI621_001641"
     CDS             5257..8619
                     /gene="mscK"
                     /locus_tag="SI621_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HEI4064371.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8658..8822)
                     /gene="rsmS"
                     /locus_tag="SI621_001642"
     CDS             complement(8658..8822)
                     /gene="rsmS"
                     /locus_tag="SI621_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HEI4064372.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8836..9363)
                     /gene="priC"
                     /locus_tag="SI621_001643"
     CDS             complement(8836..9363)
                     /gene="priC"
                     /locus_tag="SI621_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HEI4064373.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNMAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9433..9810
                     /locus_tag="SI621_001644"
     CDS             9433..9810
                     /locus_tag="SI621_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HEI4064374.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            9963..10514
                     /gene="apt"
                     /locus_tag="SI621_001645"
     CDS             9963..10514
                     /gene="apt"
                     /locus_tag="SI621_001645"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEI4064375.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10643..12574
                     /gene="dnaX"
                     /locus_tag="SI621_001646"
     CDS             10643..12574
                     /gene="dnaX"
                     /locus_tag="SI621_001646"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEI4064376.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    11907..11971
                     /gene="dnaX"
                     /locus_tag="SI621_001646"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12627..12956
                     /locus_tag="SI621_001647"
     CDS             12627..12956
                     /locus_tag="SI621_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEI4064377.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            12956..13561
                     /gene="recR"
                     /locus_tag="SI621_001648"
     CDS             12956..13561
                     /gene="recR"
                     /locus_tag="SI621_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEI4064378.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13671..15545
                     /gene="htpG"
                     /locus_tag="SI621_001649"
     CDS             13671..15545
                     /gene="htpG"
                     /locus_tag="SI621_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEI4064379.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15726..16370
                     /gene="adk"
                     /locus_tag="SI621_001650"
     CDS             15726..16370
                     /gene="adk"
                     /locus_tag="SI621_001650"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEI4064380.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16502..17464
                     /gene="hemH"
                     /locus_tag="SI621_001651"
     CDS             16502..17464
                     /gene="hemH"
                     /locus_tag="SI621_001651"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEI4064381.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17461..18420)
                     /gene="aes"
                     /locus_tag="SI621_001652"
     CDS             complement(17461..18420)
                     /gene="aes"
                     /locus_tag="SI621_001652"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HEI4064382.1"
                     /translation="MKLENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18572..19876
                     /gene="gsk"
                     /locus_tag="SI621_001653"
     CDS             18572..19876
                     /gene="gsk"
                     /locus_tag="SI621_001653"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HEI4064383.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20006..21682)
                     /locus_tag="SI621_001654"
     CDS             complement(20006..21682)
                     /locus_tag="SI621_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HEI4064384.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(21920..23140)
                     /gene="fsr"
                     /locus_tag="SI621_001655"
     CDS             complement(21920..23140)
                     /gene="fsr"
                     /locus_tag="SI621_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HEI4064385.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKE"
     gene            23358..25010
                     /gene="ushA"
                     /locus_tag="SI621_001656"
     CDS             23358..25010
                     /gene="ushA"
                     /locus_tag="SI621_001656"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEI4064386.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLDVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKINGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25047..25526)
                     /gene="ybaK"
                     /locus_tag="SI621_001657"
     CDS             complement(25047..25526)
                     /gene="ybaK"
                     /locus_tag="SI621_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HEI4064387.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25730..26524)
                     /locus_tag="SI621_001658"
     CDS             complement(25730..26524)
                     /locus_tag="SI621_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEI4064388.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISTSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLSQMLR"
     gene            complement(26953..29457)
                     /gene="copA"
                     /locus_tag="SI621_001659"
     CDS             complement(26953..29457)
                     /gene="copA"
                     /locus_tag="SI621_001659"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HEI4064389.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMLGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            29720..30652
                     /gene="glsA"
                     /locus_tag="SI621_001660"
     CDS             29720..30652
                     /gene="glsA"
                     /locus_tag="SI621_001660"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEI4064390.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            30655..>31941
                     /locus_tag="SI621_001661"
     CDS             30655..>31941
                     /locus_tag="SI621_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEI4064391.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     IGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKT"
BASE COUNT         7681 a         8483 c         8461 g         7316 t
ORIGIN      
        1 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa
       61 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      121 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      181 acgccagcga ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt
      241 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg
      301 cgttcttcgc cgacaaccca atggttgtga gcaggcctac ctggaagtaa acgtcattgg
      361 tcaggccacg gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca
      421 taacggagaa cggaatcgac cagctctcgt acagcgccgc cagacacagg aacacgacaa
      481 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca
      541 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct
      601 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
      661 ggccgttgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg
      721 agaacggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
      781 gcatacggta tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa
      841 agtcgttcac atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
      901 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
      961 ccagaccgtt tggacgtacg ctggtcaaca tatcagggtg cttcgctgct tctgcaagca
     1021 gctggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1081 agtcaaagcc ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca
     1141 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1201 tttcttcacc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1261 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1321 ctttggtcag atagtaattc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg
     1381 cacctgctgg cagctgaacc atggtcataa atacgccctg gtcttcatct ggcaagaagg
     1441 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca
     1501 ggtaacgccc tgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     1561 tctcgaacat gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     1621 tggcaatcgg tttcagcatg gtggcacaaa gagccggagt caggatcaac gccaccagta
     1681 ctgacagcgc cattgctgaa acaatggtaa tagagaactg gcgatagata gcaccagtag
     1741 aaccgccaaa gaaggccatc ggaacgaata ccgccgacag taccatcgcg ataccgacca
     1801 gagcgccctg aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt
     1861 ccgccatcac acgctcaacg ttttctacca caacgatggc gtcatccacc aacaggccga
     1921 tggcgagcac catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga
     1981 cggcaaaggt ccccagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2041 agttctgcaa gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2101 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt
     2161 tcagacctga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2221 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt
     2281 taaactctgc gatgatgtcg tagttctcac cacccagctc aattttcgcc acatcacgca
     2341 gcagcacgcg ggaaccatcc tgattcactt tcagcaggat tttgccaaac tcttcagtag
     2401 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2461 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     2521 caaccggtgt tagttggaat ttgttcagtt cattcgggtt catccagata cgcatcgcgt
     2581 actgtgaacc gaacaactga acgtcaccca cgcccgacgt acggctgatg gcatctttca
     2641 tattcgccgc cacgtagtcg gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     2701 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgta
     2761 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     2821 tatccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     2881 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     2941 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3001 ccggcggagc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3061 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3121 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3181 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3241 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3301 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3361 ccaaccacca gtacggtggc atcgccacgc ggcgtacggg ttacgccctg ttgcgggact
     3421 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3481 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccggt ggtctgatca
     3541 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     3601 atcagcgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     3661 ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     3721 tgcacggttg ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     3781 gacttaccaa tgcgaccgct aatcggagag gtaactttgg tgtaagccag attgatccgc
     3841 gcagtttcaa cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc
     3901 agagcctgat cgtactcttg cttactgatg tactgagtac cgagcaattt ctgataacga
     3961 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc gcctttcgcg
     4021 ctgtcatatg ccgcctgata ggtcgcagga tcaatctgat agagagagac acctgcttcg
     4081 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4141 tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca
     4201 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4261 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4321 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4381 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4441 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4501 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     4561 agggggtatc atccacctcg ctgggtgaga ttgcaaaagc agctggcgtt acgcgcggtg
     4621 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     4681 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     4741 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacggcgtc
     4801 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     4861 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     4921 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta
     4981 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5041 ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca
     5101 cgcttcgtaa tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5161 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5221 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5281 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5341 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5401 gcacaacttg actcactgaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5461 caggatctga cagatacatt agccaccctc gataaaatcg atcgcgtaaa agaagagaca
     5521 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     5581 ttaactgcac ttagcgatgt cgataatgac gaagaaacgc gcaaaattct gagtacgctt
     5641 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca gaacgcacaa
     5701 aacgatctgg cgtcttataa cagccagttg gtttcgttac agacgcagcc cgaacgcgtg
     5761 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     5821 actgatgtcg gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag
     5881 gctttgctga atgccgagat tgaccagcag cgtaaaagcc tggaagggaa cacagtctta
     5941 caggatacct tgcaaaagca acgggattac gtgacggcga acagcgcccg tctggagcac
     6001 cagttgcagc tgttgcaaga agcagtaaac agcaagcgcc tgactttaac cgaaaaaacg
     6061 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaag
     6121 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6181 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6241 gaacgcaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6301 ctttaccagc aacaacaaac gctgccctcg gcggatgagc tggaaaacat gaccaaccgc
     6361 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6421 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6481 cacgatgcgt tattgcaagt agttgatatg cgtcgcgaat tgctggatca gcttaacaaa
     6541 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     6601 agtgtgtcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     6661 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     6721 tcgatgaaaa tcacagtgaa ctgggaaaaa gcatggcctg ccgtttttat cgctttcctc
     6781 gccggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa
     6841 gcgtatcaac agaaactggc ttccgctgtg ggttccctgc gtaacgacag ccagctcaac
     6901 acaccaaaag cgatccttat cgacctgatc cgtgcgctgc cggtgtgcct gattattctc
     6961 gcggttggcc tgattctgtt gaccatgcaa ctcaacatca gcgaactgct atggtcgttc
     7021 agtaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7081 aacggcgttg ccgtgcgtca ctttggtatg ccggaacagc agaccagcca ctggcgtcgg
     7141 caaattgtcc gcatcagtct cgcattgctg cctatccatt tctggtctgt ggtggcagaa
     7201 ctttccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg
     7261 ctgctgattg ccttcctggt atggccgatg tgccgcgaaa gctggcgtga taaagagtcg
     7321 cacaccatgc gactggtcac cattaccgtg ctgtcgatta tcccgattgc gttgatggtg
     7381 ctgaccgcca cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc
     7441 gtttatttgg tgatcatctg gaacctgctg taccagacgg tattgcgcgg cttaagcgta
     7501 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     7561 ggcgcggaag gtgctgaacc gccggaagaa cccaccattg cactggagca ggttaaccag
     7621 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     7681 atttggtccg atttgatcac cgtgttcagc tatctggaca gcatcacgct ctggcattac
     7741 aacggcactg aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     7801 gcgattatcg cctcaatggt agcctgggcg ttgattcgca acctgcctgg tctgttggaa
     7861 gtgttggtgc tctcgcgcct gaacatgcgg cagggcgcgt cgtatgccat tactaccatc
     7921 cttaactaca tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctcg
     7981 tgggataaac tccagtggct ggcggcagca ttatccgtag gtcttggttt tggtttacaa
     8041 gagatcttcg gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgcatt
     8101 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8161 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8221 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8281 gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8341 cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcgtt tggtgccagc
     8401 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc
     8461 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgatatcaa cattgccttt
     8521 aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtcaaacgc
     8581 gactacaaag gcgacgatcc gacaccagcg gtagggtaag gacaataaag ggcgacagtg
     8641 atgccgccct ttatttttta ctcgctttgc gaagcctcat cgctctgtaa cttcttttca
     8701 taatcacgct tcacaatatc cagcgcgcgc agcacggtgc gggctgggat ctgattttct
     8761 tccagcagca caatcaaatc gacagccagt ttgacatcgt caggggcatt ttccagtgac
     8821 atactctctc cattgctagc gggttaagcg cgctagcctg ttttcgattt tttccagcgc
     8881 atggcggcag cgtgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg
     8941 ttccacgaga tcggtcaccc gcgccaggcg ggctcgacgt tcggctacca tctcacgtag
     9001 ccgccgctca aaatcctgat gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg
     9061 cgcactgtcc cactcgcgta gcgaccaggc tgtggcttca cgcgctatag cctccagttg
     9121 cgccgccaga tgttctgcca gccaggccac ttgcggcagc tgttgctgct caaccgcatg
     9181 acgaagcgca gccatgttat cgcccgcctc atcgagacaa gcctgcagtg ttgtcgcacg
     9241 ggtctgaaaa aggtgcctgt caaagcgggc acttagcgtg gcaaattgcg ctaccggggc
     9301 acaacgctgg cgcagcgtgg cgagctgtcc ttccagtttt tccagcagca gggcggtttt
     9361 catgataaga cctcaatgaa aatgataatt gttatgctaa agtagccact ctgtaagctg
     9421 acaacactca atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg
     9481 ggtacgctgg gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctggcggcc
     9541 tggtgctttg cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt
     9601 ggcagctatc tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg
     9661 gcgattttgc ttattttgct cacgtttgca atttctctgt ggttcgtcca gatgccatgg
     9721 gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt tttatatgtg gcgaattccg
     9781 gtgattgatg aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg
     9841 tacagccagt acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac
     9901 tgttactttt gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac
     9961 ttatgaccgc gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg
    10021 actacccaaa acccggcatt cttttccgtg atgtcaccag cttactggaa gacccgaaag
    10081 cttacgctct cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag
    10141 ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg
    10201 ttggctttgt accggtccgt aaaccgggca aactgccgcg tgaaaccatc agtgaaactt
    10261 acgatctgga atacggcacc gatcagctgg aaatccacgt tgatgccatc aaaccgggcg
    10321 acaaagttct ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta
    10381 aactgatccg tcgtctgggt ggtgaagtgg ctgatgctgc gttcattatc aatctgttcg
    10441 atctcggcgg cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgttccgt
    10501 tcccgggcca ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt
    10561 gcattcagcc tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct
    10621 tccagcgttt cagagcctgc caatgagtta tcaggtctta gcccgaaaat ggcgcccaca
    10681 aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc
    10741 gttagggcgt attcatcatg cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc
    10801 tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg
    10861 cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat
    10921 cgatgccgcc tcgcgcacta aagttgaaga cactcgcgac ctgctggata acgtccagta
    10981 cgctccggcg cgtggtcgtt tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg
    11041 ccacagcttt aacgcactgt taaaaaccct tgaagagccg ccagagcacg ttaagtttct
    11101 gctggcgacg accgatccgc agaaattgcc ggtgacgatt ttgtcacgtt gtctgcaatt
    11161 tcatctcaag gcgctggatg tcgagcaaat tcgccatcag cttgagcaca tcctcaacga
    11221 agaacatatc gctcacgagc cgcgggcgct gcaattgctg gcacgcgccg ctgaaggcag
    11281 cctgcgagat gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc
    11341 aactcaggcg gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt
    11401 tgaagcgatg gtcgaggcca acggcgagcg cgtaatggca ctgattaatg aagccgctgc
    11461 ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc ggcctgttgc atcgtattgc
    11521 gatggtacaa ctttcgcctg ctgcacttgg caacgacatg gccgctatcg agctgcggat
    11581 gcgtgaactg gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt
    11641 gattggtcgc aaagaattac cgtatgcgcc agaccgccgt atgggcgttg agatgacgct
    11701 gttgcgcgcg ctggcgttcc atccgcgtat gccgctgcct gagccagaag tgccacgcca
    11761 gtcctttgcg cccgtcgcgc caacggcagt aatgacgcca acccaggtgc cgccgcaacc
    11821 gcaatcagcg ccgcagcagg caccgactgt accgctcccg gaaaccacca gccaggtgct
    11881 ggcggcgcgc cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc
    11941 ggcagccgct acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac tggcttcggt
    12001 caccgatcgc gttcaggcgc gtccggtgcc atcggcgctg gaaaaagcgc cagccaaaaa
    12061 agaagcgtat cgctggaagg ccaccactcc agtgatgcag caaaaagaag tggtcgctac
    12121 gccgaaggcg ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct
    12181 ggcggcagaa gccattgagc gcgacccgtg ggcggctcag gtgagtcaac tttcgctacc
    12241 aaaactggtc gaacaggtgg cgttaaatgc ctggaaagag gagagcgaca acgcagtatg
    12301 tctgcatttg cgctcctctc agcggcattt gaacaaccgc ggcgcacagc aaaaactggc
    12361 tgaagcgttg agcatgttaa aaggttcaac ggttgaactg actatcgttg aagatgataa
    12421 tcccgcggtg cgtacgccgc tggaatggcg tcaggcgata tacgaagaaa aacttgcgca
    12481 ggcgcgcgag tccattattg cggataataa tattcagacc ctgcgtcggt tcttcgatgc
    12541 ggagctggat gaagaaagta tccgccccat ttgatcgtaa gcacagctta cgttcgtcat
    12601 ccttaacgtg attgagagag aaacctatgt ttggtaaagg cggtctgggt aacctgatga
    12661 agcaagccca gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg
    12721 aagtcaccgg cgaatctggc gcaggtctgg taaaagtgac cattaacggt gcacacaact
    12781 gccgtcgcgt agagatcgac ccgagcctgc tggaagacga caaagagatg ctggaagact
    12841 tggtggctgc agcattcaac gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa
    12901 tggcctctgt atcctccgga atgcagctgc cgcctggctt taagatgccg ttctgatgca
    12961 aaccagcccg ctgttaacac agcttatgga agcactgcgc tgtctgccgg gcgttggccc
    13021 gaagtcggcg cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg
    13081 tctggcgcag gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg attgccgcac
    13141 cttcaccgaa caggaagtct gtaacatctg ttcgaatccg cgtcgtcagg aaaacggtca
    13201 aatctgcgtg gtggagagtc cggcggacat ctacgccatt gagcagacgg ggcagttttc
    13261 aggccgttat tttgtgttga tggggcacct gtcaccgctg gacggcatcg gtccggatga
    13321 tatcggcctt gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg aagtgatcct
    13381 cgccaccaac cccactgttg aaggtgaagc taccgctaac tacattgccg agctttgcgc
    13441 gcaatatgac gtggaagcca gccgaatcgc tcatggcgta ccggttggcg gcgaactgga
    13501 aatggtcgat ggcaccacgc tgtcacactc ccttgccggg cgtcataaga ttcgttttta
    13561 agcaaacgag agcaggatca cctgctctcg cttgaaatta ttctcccttg tccccatctc
    13621 tcccacatcc tgtttttaac cttaaaatgg cattattgag gtagacctac atgaaaggac
    13681 aagaaactcg tggttttcag tcagaagtga aacagcttct gcacctgatg atccattctc
    13741 tctattccaa taaagaaatc ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg
    13801 acaagctgcg tttccgcgcg ctctctaacc cggacctgta cgaaggtgat ggcgaactgc
    13861 gcgttcgtgt ctctttcgat aaagacaagc gtacgctgac catctccgat aacggcgtag
    13921 ggatgacccg cgacgaagtg attgaccatc tggggactat cgctaaatcc ggcaccaaat
    13981 cattcctcga atccctgggt tctgaccagg cgaaagacag ccagctgatc ggtcagtttg
    14041 gtgttggttt ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt actcgcgcgg
    14101 caggcgaaaa accagaaaac ggtgttttct gggaatcggc tggtgaaggt gaatacaccg
    14161 ttgccgacat caccaaagaa gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg
    14221 aagacgagtt cctcgatgac tggcgcgtgc gttccatcat cagcaaatac tccgaccata
    14281 tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc gttatctctt
    14341 gggaaaaaat caacaaagcg caggcgctgt ggactcgtaa caagtcggaa atcaccgatg
    14401 aagagtacaa agagttctac aaacacatcg cccacgactt taacgatccg ctgacttgga
    14461 gccacaaccg cgttgaaggt aagcaggagt acaccagcct gctgtacatt ccgtctcagg
    14521 caccgtggga tatgtggaac cgcgatcata aacacggtct gaaactgtat gttcagcgtg
    14581 tgttcatcat ggacgacgca gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc
    14641 tgattgactc cagcgatctg ccgctgaacg tttcccgtga aatcctccag gacagcacgg
    14701 taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg
    14761 cgaaagacga cgcggaaaaa taccagacct tctggcaaca gtttggcctg gtactgaaag
    14821 aaggtccggc ggaagatttc gctaaccagg aagcgatcgc caaactgctg cgttttgctt
    14881 ctacccatac cgattcttct gcgcagaccg tgtctctgga agactacgtt tcccgcatga
    14941 aagaagggca ggagaaaatc tactacatta ccgcagacag ctatgcggca gcgaagagca
    15001 gcccgcacct ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca
    15061 tcgatgagtg gatgatgaac tatctgactg agttcgacgg taaaccgttc cagtcggtgt
    15121 ctaaagttga cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag
    15181 cggagaaagc actgactccg ttcatcgacc gtgtgaaagc cctgctcggc gagcgtgtga
    15241 aagatgtccg tctgactcac cgtctgaccg atacgccagc gattgtctct accgatgcgg
    15301 acgaaatgag cacccagatg gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag
    15361 tgaaatacat ctttgaactg aacccggatc acgtactggt gaaacgtgcg gcagatactg
    15421 aagatgaagc gaagttcagc gagtgggtag aactgctgct ggatcaggcg ctgctggcag
    15481 aacgcggcac gctggaagat ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt
    15541 cctgatgtca tgccggatga ccttcgtgtc atccggcatt tttcttttca ttatctgcac
    15601 tttccgcaaa ttatctcgcc attaaccgtt tcagccccag gtgcctttct tgaggcaatc
    15661 gcctgttggt ggtatcgttt atcgcttttt caaaaaattc gacacatttt aaggggattt
    15721 tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg gaaagggact caggctcagt
    15781 tcatcatgga gaaatatggt attccgcaaa tctccactgg cgatatgctg cgtgctgcgg
    15841 tcaaatctgg ctccgagctg ggtaaacaag caaaagacat tatggatgct ggcaaactgg
    15901 ttaccgacga actggtgatc gcgctggtta aagagcgcat tgctcaggaa gactgccgta
    15961 atggtttcct gttggacggc ttcccgcgta ccattccgca ggcagacgcg atgaaagaag
    16021 cgggcatcaa tgttgattac gttctggaat tcgacgtacc ggacgaactg atcgttgacc
    16081 gtattgtcgg tcgccgcgtt cacgcgccgt ctggtcgtgt ttatcacgtt aaattcaacc
    16141 cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga actgactacc cgtaaagacg
    16201 atcaggaaga aaccgtacgt aaacgtctgg ttgaatacca tcagatgact gcaccgctga
    16261 tcggctacta ctccaaagaa gcggaagcgg gtaacaccaa atacgcgaaa gttgacggca
    16321 ccaagccggt tgctgaagtt cgcgctgatc tggaaaaaat cctcggctaa tcaagccgca
    16381 gcagatctgc tttcgggcag gtctgctcat tctcctcagg caatacctct cacagcaatt
    16441 agttcttctt cttcattttt ccgctacaat tatcaacaag ttgaatcgat aagaggcggt
    16501 aatgcgtcag actaaaaccg gtatcctgct agcaaacctg ggtacgcccg atgcccccac
    16561 acctgaagcg gtaaaacgct atctgaaaca atttttaagc gacagacgcg tggttgatac
    16621 ctcacggttg ttatggtggc cattgctgcg cggcgtgatt ttgccactgc gctcgccgcg
    16681 tgtggcgaag ctgtatgcct ctgtctggat ggagggtggc tcgccgctga tggtttacag
    16741 ccgccagcaa cagcaggcgc tggcacaacg tttaccggag acgcccgtag cgctgggaat
    16801 gagctacggc tcgccatcac tggaaagcgc cgtagatgaa ctcctggcag agcatgtaga
    16861 tcatattgtg gtactgccgc tttatccgca atactcctgt tcaacggtcg gtgcggtatg
    16921 ggatgaactg gcacgcattc tggcgcgcaa acgtagcatt ccggggatat cgtttattcg
    16981 tgattacgcc gataaccacg attacattaa tgcactggcg aacagcgtac gtgcttcttt
    17041 cgctaaacat ggcgaaccgg atctgctgct gctctcttat catggcattc cccagcgtta
    17101 tgcagatgaa ggcgatgatt atccgcaacg ttgccgcaca acgactcgcg aactggcttc
    17161 cgcactgggg atggcaccgg aaaaagtgat gatgaccttt cagtcgcgct ttgggcggga
    17221 accctggctg atgccttata ccgacgaaac gctgaaaatg ctcggagaaa aaggcgtagg
    17281 tcatattcag gtgatgtgcc cgggctttgc tgcggattgt ctggagacgc tggaagagat
    17341 tgccgagcaa aaccgtgagg tcttcctcgg tgcgggcggg aaaaaatatg aatatattcc
    17401 ggcgcttaat gccactccgg aacatatcga gatgatggct aatcttgttg ccgcgtatcg
    17461 ctaaagctga gcggtaaaga actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat
    17521 catccgtgaa taatgcaaaa aggcgtgcag cgtgcctggg tagagtttga actcacaggg
    17581 ctgctgatgc gccgctaacg tctggtaaag cagacggctg tcatccagca gcggatcgaa
    17641 ctccgccccg gcaataaaac agggcggaac ttcgcgagtg agatcattat taaacaggca
    17701 gtaatacggc gactcgcggt ccgcgtcgtt gcttaaatac gcctcttcat acatctgtaa
    17761 atcctgttgc gttaagccat cccagacacc gcccaacaga cgacgagtca cggaatcccg
    17821 taatccgtaa agcccatacc acagcaaaac gcccgcaact ttaccgcaat cgatctgttt
    17881 atcacgcaac cacaacgcac tggcgagcgc cagcatggca cctgcggaat caccggcaaa
    17941 gccaatgcgg gacatattga tttgataatc ctccgcctgc tggtggaaat aacaacaagc
    18001 agccacaatt tcctctatcg cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat
    18061 accaatcacc gtacattggc tgtagcttgc cagcaggcgc atgatgcgat cgtgggtatc
    18121 gagattgccg agaataaaac cgcctccatg caaataaaat agcgtcgctg ggctatctgg
    18181 ctgcggacaa aagagacgtg tttccacctg cccatatttt gttggaacca tgtaagctct
    18241 ggttgccatt tctggagcgc ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg
    18301 ttgctcagca atcgttcccg ttgcgggcca gggcggtaaa tccggctgaa gagtattcac
    18361 aacggtcttc atttcagcag aaataaggtc cagaacgggt aatttgtttt ccagcttcat
    18421 gcaaaaactc ctttcaaatt acgtcattgt aaggaaccac tgccatgaaa atgcgatccc
    18481 gcctgctgat attgaaactg gctgcgtctc gcgcgctccc gtcagattgt gttaacattc
    18541 gccgctcagt taaccacccg taaaaacaac catgaaattt cccggtaaac gtaaatccaa
    18601 acattacttc cctgtaaatg cacgcgatcc gctgcttcag caattccagc cagaaaacga
    18661 aaccagcgcc gcctgggtag tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt
    18721 ggatgacgaa ttcattgagc gttatggatt aagcgccggg cattcactgg tgattgagga
    18781 tgacgtagcc gaagcgcttt atcaggaact aaaacagaaa aacctgatta cccatcaatt
    18841 tgcgggtggc actattggta acaccatgca caactactcg gtgctcgcgg acgaccgttc
    18901 ggtgctgctg ggcgtcatgt gtagcaatat tgaaattggc agctatgcct atcgttacct
    18961 gtgtaacacc tccagccgta ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg
    19021 tcgttgcttt acgctgattg gcgagtccgg ggaacgtacc tttgctatca gccctggcca
    19081 catgaaccag ctgcgggctg aaagtattcc ggaagatgtg attgccggag cctcggcact
    19141 ggttctcact tcttatctgg tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat
    19201 gaaagccatt gagtacgcga agaaatataa cgtaccggtg gtgctgacgc tgggcaccaa
    19261 gtttgtcatt gccgagaatc cgcagtggtg gcagcaattc ctcaaagacc acgtttctat
    19321 ccttgcgatg aacgaagatg aagccgaagc gttgaccgga gaaagcgatc cgttgttggc
    19381 atctgacaag gcgctggact gggttgatct ggtgctgtgc accgccgggc caatcggctt
    19441 gtatatggcg ggctttactg aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc
    19501 gggcgctata gcggaattta accagtatga gtttagccgc gccatgcgcc acaaggattg
    19561 ccagaatccg ttgcgtgtct attcgcacat tgcgccgtat atgggcgggc cggaaaaaat
    19621 catgaacact aatggagcgg gggatggcgc actggcagcg ttgctgcatg acattaccgc
    19681 caacagctac caccgtagca acgtaccaaa ctccagcaaa cataaattca cctggttgac
    19741 gtattcgtca ttagcgcagg tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa
    19801 ccagcattca cctcgtttaa cgcgcggctt gccggagcgt gaagacagcc tggaagagtc
    19861 ttactgggat cgttaagtta tcgtcggttc gtaggccaga taaggcgttc acgccgcatc
    19921 tggcatttgg ctctcgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacatatcc
    19981 gcgcctatta tccggcaacc accgtttacc ccgtcaccac ctcacccgcc ggtggcgttt
    20041 caagcagttc cagcatagta cgggcgattt cacgctcacc catcactacc tgatttgcac
    20101 cacgttcggt gatatacgcc acttcatcgt cataatgggc gcgggcaata atctcaatat
    20161 ccggattttt cgcgcgggca gatgccacaa tctcacccgc ttcataaccg ttgggaatcg
    20221 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgccg
    20281 cattgcccaa tactgcgcgg accccgcgct ctcgcagttc atcaacacgg gttcgtgacg
    20341 tctcaatcac cactaaagga atatcagagg cgagcaattt ctcccccagc aggctgccta
    20401 cacgaccgta acctaccagt agcgcatggt tgcaaatatc cactgggatc tgcttctctt
    20461 cttcgatagc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    20521 ccagcagcgc aaacaggacc gggttgagca taatcgacaa aatcgcccct gccagtacca
    20581 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    20641 cgaactcacc aatctgcgcc aggctggcgg cgatagttaa cgccgtgcgc tgggagtgac
    20701 caaacagacg cacaaggaac agtgccgcga ccgatttacc aaacagaata atcgccagcg
    20761 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    20821 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    20881 gactcagttc agactcgttc agcaccatcc cggcaaagaa cgcaccgagt gcaaaggaga
    20941 catcaaacag ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    21001 taaacagctc acgggaaccg gttgctgcgc tgcgtgccat aatccacggc accagacggc
    21061 gacctaccag catcataatg gcgataaatg cgatcacctt gccgatggtg atccccatat
    21121 cgacagcaag agtggcaaag cccacatcgc cttgttccat cattcctgcc actgcgggca
    21181 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    21241 gcccacgctg actgtctatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    21301 tggcggtgga aaggcataag ccgaacacga taccggtcat taacgaccag cccagcacgg
    21361 cggagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    21421 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    21481 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    21541 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    21601 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    21661 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    21721 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgtttatgaa
    21781 taattgttga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    21841 tgaaggcggt ttaacagagg aaaggcttat ggcgactgta aaatgatgca gccaaagttt
    21901 gcctttggct gcaatgaaat cactctttat gccggttatc aggcaggaat atggtcaaca
    21961 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgctg gtgtggtcgg
    22021 cgataagccc cagaactgcc gctcccagac ctcccatgcc aaaagcaaaa ccgaaaaaga
    22081 gtccagaaac cataccgata cgtcccggaa gcagctcctg agcgtagacc agaatggcag
    22141 agaatgccga agcgaggata aatccaataa tcaccgttaa aacccccgtc cagtgcaggc
    22201 tggcgtaggg taaaatcagc gtaaacggcg caacgccgag gatagagccc caaatcacat
    22261 attttcgccc aattttatcc cctacaggcc cgccgatcac cgtaccggcc gcaacggcaa
    22321 acaggaaggc aaacagatga agctgggcat tctggataga taatccgaat ttttgcatca
    22381 gataaaaggt gtaatagctg ctgatgctcg ccatatagaa atatttcgag aaaatgagga
    22441 ttaacagaat gctgaccgcc agtaccactt tgttgcgcgg cagtggattg ataatcgtcg
    22501 cttttggttt tcctttattc attcggtgct gtgccgagta ccaacggctg atttgcgcca
    22561 acaccacgat cgccagcagt gccgcaagta caaaccaggc tacgttgcct ttgccataag
    22621 gcgcgataat taccgctgcc agcaagggcc ccagggaact gccaaagttg ccgccgacct
    22681 gaaagataga ttgcgccagg ccatgacgcc cgccagaagc catacgggcc acgcgagaag
    22741 attccggatg aaagaccgat gaaccggtac cgaccagcgc cgccgccagc agaactgcgc
    22801 caaaactgcc cgccagcgca agcagcacca gaccacttaa ggtaaagcac atgccaattg
    22861 gcaacgacca cggcatcgga tatttatcgg tccagtaacc gaccaccggt tgcagtagcg
    22921 aagaggcgag ctggaaggtg agggttatca tgccaatctg cataaatgtc agagaaaact
    22981 ctgactgaag cagcggataa atcgccagaa tcagggattg gatcatgtcg ttcagcagat
    23041 gtgagaggct gatagcacct aaaataccaa acgatgttcg ggccttggtc gttgacgcag
    23101 ccgcgcccgc cacaggctgg gtttgttcac tcattgccat aggaaagtca ctttttcagg
    23161 gttgcgatgt aaagaatgat cttatttgtg attattaccc gactaacata cctgtatgcg
    23221 tcgcctgaag gaagtctcaa cgccgaatac agaatttcta atctggatgc agatttatct
    23281 tcaccggacg cagacttgtc tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc
    23341 aggtcaggga gagaagtatg aaattattgc agcggggcgt ggcgttagca ctgttaacca
    23401 catttacact ggcgagtgaa gctgctctgg cgtatgagca ggataaaacc tacaaaatta
    23461 cagttctgca taccaatgat catcatgggc atttttggcg caatgaatat ggcgaatatg
    23521 gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg
    23581 gtagcgtgct gctactttcc ggtggcgaca ttaacactgg cgtgcccgag tctgacttac
    23641 aggatgccga acctgatttt cgcggcatga atctggtggg ctacgatgcg atggcaatcg
    23701 gtaatcatga atttgataac ccgctcaccg tattacgcca gcaggaaaag tgggcaaagt
    23761 tcccgttact ttctgccaat atctaccaga aaagtaccgg cgagcgcctg tttaaaccat
    23821 gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat tggcctgacg acggatgaca
    23881 cagcgaagtt aggcaaccca gaaaacttca ccgatattga gttccgtaag cccgccgatg
    23941 aagcgaagct ggtgattcag gagctgcaac agacagaaaa gccagacatt attatcgcgg
    24001 cgacccatat ggggcattac gacaatggtg agcacggctc taacgcaccg ggcgatgtgg
    24061 agatggcgcg cgcgctgcct gccggatcgc tggcgatgat cgtcggtggt cactcgcaag
    24121 ctccggtctg catggcggca gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat
    24181 gcaaaccgga tcaacaaaac ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg
    24241 tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa aatggttaac taccagctga
    24301 ttccggtgaa cctgaagaag aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt
    24361 acactcctga aatcgctgaa aaccagcaaa tgatctcgct gttatcaccg ttccagaaca
    24421 aaggcaaagc gcagctggac gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc
    24481 gtgacaaagt gcgttttgta cagaccaata tggggcggtt aattctggca gcccaaatgg
    24541 atcgcactgg tgctgacttt gcggtgatga gcggaggcgg aattcgtgat tctatcgaag
    24601 caggcgatat cagctataaa aacgtgctga aagtgcagcc attcggcaat gtggtggtgt
    24661 atgccgacat gaccgggaaa gaggtgattg attacctgac cgccgtcgca caaatgaagc
    24721 cagattcagg tgcctacccg caatttgcta acgttagctt tgtggcgaaa gacggcaaac
    24781 tcaacgacct gaaaatcaac ggcgaaccgg tcgatccggc gaaaacttac cgcatggcga
    24841 cattaaactt caatgccacc ggcggtgatg gctatccgcg ccttgataac aaaccgggct
    24901 atgtgaatac cggctttatt gatgccgaag tgctgaaagc gtatatccag aagagctcgc
    24961 cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg
    25021 ccggatattt gcatccggca caatgcttaa tcgcggcggg cgatatcagc aaatttggca
    25081 tcgaggatct ttgccagatc gcctgccgcc agttcgatat ccagcccgcg cttgccgccg
    25141 gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt
    25201 ttctgcccca gtgggctaat ccccccaacc agatatcccg tcgaacgctg cgcaaccatc
    25261 ggatcggcca tctcaacttt cttggcaccc agcgcttttg ctactttttt aagatccagt
    25321 tgacctgcga ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgcgacc
    25381 agcagcgttt tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca
    25441 aaattggttt cagccggatc gtgctcgtag gtatggattt gaaacgaaat cttgtttttt
    25501 tcgagtaatt taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc
    25561 aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg
    25621 atggtgagat tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa
    25681 aaaaatgaaa ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca
    25741 tctgcgacaa attcccttct ccatacaggt gtagtgcacc gactgcgacc acatagcgcc
    25801 ccggcggcat ggcgcataat ttgtcccgcc aggcgagatt tcgctgatgc atcagcacat
    25861 cgtacagcga ttgactgaac gtattaggca gcgttatttc attattttgc ggcggcgcat
    25921 tcagccacca gctcatcatt tgttgcagca accgtgcatt ggtgtgccag tgggtcagcg
    25981 tatcgtccag cagtgccagt ccgttgtcag ggagctggag caacatggca atctggtttt
    26041 cagccccttc cagttcaatc acgggtttat gttgttgctt cgccgcctgc aatagctggt
    26101 aatcgatacc gtattctgcc cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca
    26161 tcgcgatttg ccacagcggt tgggtagaaa agagtgaggt agaaatgccc atctcctggc
    26221 taatgtgctg caggttttgg agttgctcct cgctaatgcg ctcttccagc gcctcgcagg
    26281 caggcaaatt agcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat
    26341 cggcgttttt gagctttttg agcaaacggg tgggcagggg agccatatcg tggctgccca
    26401 tatgaatact gccaatcaga tgaaaatggc gattgccggg aagagagata tcgatagcag
    26461 gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat
    26521 ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg gggagggatg gcgcaaatgg
    26581 agttatgagg cttgttcagt ggtagcttaa atatgatcga aaaattgttt ttgctttgaa
    26641 acgaaagact gcaccggtgt tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg
    26701 ggcagcccac atggatgtgg gctgagggcg cgttttacag ggatgttacc tcgcgcccga
    26761 cccggtagcc gcaagggata agctgagggt accgcgcagc ggcgattttg ttcgccagag
    26821 cccgggggtg cagggggcgg cggcgattgg ccgccccctg cgcgctccct gcgccagtca
    26881 ctttatatgg cattactcat aaaaggagcg caacaagaac tggatctcac aaacgcatta
    26941 agtaaatacc aatcattcct tcggtttaaa ccgcagcaac cggttggcgt tactcactac
    27001 ggtaatcgac gagagcgcca ttgccgctcc ggcgaccacc gggttaagca gtgttccagt
    27061 gaacggccac aaaataccgg cggcgaccgg aataccgata ctgttgtaga taaacgcgcc
    27121 gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac
    27181 acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa catcactgcc
    27241 gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt taatgccgtc
    27301 gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt cggctttacc
    27361 gtccggtagc accccggcta tcacctcatc aatccctgct tctttggcga tcgcattggc
    27421 ggtggttggg ttatccccgg ttagcatcac cagacgatat cccgctttat gcaggcgttg
    27481 cagcgccgcc acgctatcac tacgcaatgg atcgcgtaca gccagcaggg ctaccgcttt
    27541 cccgtcaacc gccagcagca caggcgttgc cccttgtgat gcctgagctg aaatatctgc
    27601 ctcaatcgct ttagtatcaa cttgctggtc atttaacagc gcctgattgc ccagcaatac
    27661 cgcatgacct tcagcttcac cgctcacacc cagcccgcgc aatgtgcgga aaccgttgac
    27721 ctgcggtagc tgcatatcac ctgctttatc gaggatcgcc cgtgccagcg ggtggctgga
    27781 accttgctcc agcgccgccg ccagacgcaa tgcctgcgct tcatcaacat cagcaaatgt
    27841 ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat cgaacaccac
    27901 ggtgtcgagc gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca caccaaactc
    27961 agccgcccgc ccgacgcccg aaataatcga catcggcgtc gccagtccca gcgcacacgg
    28021 acaggcgata atcagcaccg tggtggcaat caccagggta tagacaatct gcggtgccgg
    28081 accaaagaaa taccagattg ccgcactgat cagcgcaatg actaccacta ccggcacaaa
    28141 cacggcggag attttatccg ccagttgacc gatttctggc ttgctgctct gggcctgtcg
    28201 caccatgcga atgatccgtg acagcgtagt atggctgccg accgcactgg cacgaaacag
    28261 cacactgccg tcctgtacca ctgtcccggc atggacgcta tcgccttcgc ctttttgctg
    28321 cgggataggt tcgcccgtca gcatcgcttc atccagccac gcttcgcctt gggtaatctc
    28381 accatctacc ggcacacggt cgccggtcgt caggcgcagt aacatacctg gctgaacatc
    28441 tgccagcggc acgttttttt caccttcgtc ggtgaccagg cgcgccgtcg gtggggttaa
    28501 atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt ccagcatatg
    28561 gccgagattg atcagaccga taatcatcgc gctggcttcg taataaagat gtcgcgcttc
    28621 catcgggaac cactgcggcc acaggttgac gctcatcgaa tagagccacg ccacgccagt
    28681 acctagcgcc accagcgtat ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact
    28741 acggtaaaaa tggccgccgg cgaaaaccat cactgccagg gttatcaggc cgataactaa
    28801 ccacaagctg cggttgtcag cggtgaccat catgttatcg ccgagcatcc cccagaccat
    28861 caccgggata cccaccgcca gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc
    28921 gactgcggtt tcctgctggc gctcgcggcg tttagcgtca tcttcaatcg cttccgcgcc
    28981 gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc ccatcaccag
    29041 cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc tttgcaacgc
    29101 attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca actgctggct
    29161 gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca gtgcttccga
    29221 cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg cgtcataacc
    29281 cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc
    29341 agtgacagac acatccgcct gctcaacatc cgggcgctgt tcgagacttt ctttcacgcg
    29401 tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt gtgacataaa
    29461 acactccttt taagacagtt ttgactggct gtgataaagg ttaaaccttc cagcaagggg
    29521 aaggtcaaga aattaataaa ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc
    29581 gatttgcatc aggattagac atttatctct ttgttttcct gtagttaagt tgcgggtgct
    29641 aagttaaatc aggatgcctg aaaatcggca ccggggtgag gaattacctc ccgcatctat
    29701 aaaaaggagt taacaaaaga tgttagatgc aaacaaatta cagcaggcag tggatcaggc
    29761 ttacacccaa tttcactcac ttaacggcgg acaaaatgcc gactacattc cctttctggc
    29821 gaatgtacct ggtcaactgg cagcagtggc tattgtgacc tgcgatggca acatctatcg
    29881 cgcgggtgat agtgattacc gttttgcgct ggaatccatc tcgaaagtct gtacgttagc
    29941 ccttgcgtta gaagatgtcg gcccgcaggc ggtacaggac aaagttggcg ctgacccgac
    30001 cggattgccc tttaactcag ttatcgcctt agagttgcat ggcggcaaac cgctgtcgcc
    30061 actggtaaat gctggcgcta ttgccaccac cagcctgatt aacgctgaaa atgccgaaca
    30121 acgctggcag cgaattttac atatccaaca acaactggct ggtgagcagg tagcgctctc
    30181 tgacgaagtc aaccagtcgg aacaaacaac caacttccat aaccgggcca ttgcctggct
    30241 gctgtactcc gccggatatc tctattgtga tgcaatggaa gcctgtgacg tgtacacccg
    30301 tcagtgctcc acgctcatca atactgttga actggcaacg cttggcgcga cgctggcggc
    30361 gggtggtgtg aatccgctga ctcataagcg cgttcttcag gccgacaacg tgccgtacat
    30421 tctggctgaa atgatgatgg aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt
    30481 tggtttaccg ggcaaaagcg gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat
    30541 gggaattgcc gcgttctcac caccgctgga cgaagaaggc aacagtgttc gcggtcaaaa
    30601 aatggtggca tcggtcgcta agcaactcgg ctataacgtg tttaagggct gatcatgatg
    30661 aacacggaag gtaataacgg taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt
    30721 attggggcaa tggtgggggc ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg
    30781 gaagcctcga cctgggtcgc ctttgcattt ggcggtattg tggcgatgtt ttccggttat
    30841 gcctatgcgc gcctgggggc gagctatccc agcaatggcg gtattatcga cttctttcgt
    30901 cgcggattag gcaacggcgt cttttcgctg gcgctctcgt tactgtacct gttgacgctg
    30961 gcggtgagca tcgccatggt cgcccgtgct tttggcgctt atgccgtgca gtttttgcat
    31021 gaaggcagcc aggaggagca ccttatattg ctctacgcgt tggggatcat tgcggtgatg
    31081 acgcttttca actccttaag caaccatgcg gtagggcggc tggaagtgat cctcgtcggc
    31141 attaaaatga tgatcctgtt attgctgatt attgccggtg tctggtcgct gcaaccggcg
    31201 catatttctg tctctgcgcc ccccagctcc ggtgcgttct tctcctgtat cgggataacc
    31261 ttccttgcct atgcgggctt tggcatgatg gcgaacgcgg cggataaagt gaaagatccg
    31321 caggtcatta tgccacgggc gtttctggtg gcgattggcg ttaccacgtt gctttatatc
    31381 tcgctggcgc tggttttgct tagcgatgta tcggctttag agttagaaaa atatgccgat
    31441 accgccgtag cgcaggcagc ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc
    31501 atcggcgctt tactggcgac ggcttcagcc atcaacgcga acctgttcgc cgtgtttaac
    31561 atcatggaca acatgggcag cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg
    31621 cggcagagta cctggggcaa catcattgtc gtggtgctga ttatgctgat gacggcggcg
    31681 ctgaatttag gctcactcgc cagcgttgcc agcgccacct ttttgatctg ctatctggcg
    31741 gtgtttgtgg tggcgatccg cctgcgtcat gatattcacg cctcgttgcc gattcttatc
    31801 gttggtacgt tggtgatgtt gttggtgatc attggcttta tctacagcct gtggtcccag
    31861 ggcagccgtg cgttgatatg gattattggt gcaatcttac tcagccttat tgtggcaatg
    31921 gtcatgaagc gcaataaaac c
//
LOCUS       DARNVM010000040        31853 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.040, whole genome shotgun sequence.
ACCESSION   DARNVM010000040 DARNVM010000000
VERSION     DARNVM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.040"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(305..394)
                     /locus_tag="SI621_001662"
     tRNA            complement(305..394)
                     /locus_tag="SI621_001662"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Ser,seq:cga)
     gene            488..1285
                     /gene="mtfA"
                     /locus_tag="SI621_001663"
     CDS             488..1285
                     /gene="mtfA"
                     /locus_tag="SI621_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HEI4064392.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1386..1461
                     /locus_tag="SI621_001664"
     tRNA            1386..1461
                     /locus_tag="SI621_001664"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1419..1421,aa:Asn,seq:gtt)
     gene            1623..2885
                     /locus_tag="SI621_001665"
     CDS             1623..2885
                     /locus_tag="SI621_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEI4064393.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLQKGIEEKGLLEV
                     ASRTRQHLSNIIRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRGWTIPATREPIIGVHYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNSYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(3079..4383)
                     /gene="ybtS"
                     /locus_tag="SI621_001666"
     CDS             complement(3079..4383)
                     /gene="ybtS"
                     /locus_tag="SI621_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HEI4064394.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGLQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4411..5691)
                     /gene="ybtX"
                     /locus_tag="SI621_001667"
     CDS             complement(4411..5691)
                     /gene="ybtX"
                     /locus_tag="SI621_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HEI4064395.1"
                     /translation="MSDVQSNVKPLTLTTGQVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5684..7486)
                     /gene="ybtQ"
                     /locus_tag="SI621_001668"
     CDS             complement(5684..7486)
                     /gene="ybtQ"
                     /locus_tag="SI621_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HEI4064396.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            complement(7473..9185)
                     /gene="ybtP"
                     /locus_tag="SI621_001669"
     CDS             complement(7473..9185)
                     /gene="ybtP"
                     /locus_tag="SI621_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HEI4064397.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            9442..10401
                     /gene="ybtA"
                     /locus_tag="SI621_001670"
     CDS             9442..10401
                     /gene="ybtA"
                     /locus_tag="SI621_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HEI4064398.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10592..16699
                     /gene="irp2"
                     /locus_tag="SI621_001671"
     CDS             10592..16699
                     /gene="irp2"
                     /locus_tag="SI621_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HEI4064399.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQQRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            16787..26278
                     /gene="irp1"
                     /locus_tag="SI621_001672"
     CDS             16787..26278
                     /gene="irp1"
                     /locus_tag="SI621_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HEI4064400.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLKDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDRRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTVPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDGGRKSTALLLSAASDSALRRLATDYAGALRKNADASDLAFTALHAR
                     RLDLPFRLAVPLNRETADALSTWASEKSGAQVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHQALWALLRVAANEQP
                     ERLLAAIDLAENTPRETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLANALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTVGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26275..27375
                     /gene="ybtU"
                     /locus_tag="SI621_001673"
     CDS             26275..27375
                     /gene="ybtU"
                     /locus_tag="SI621_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HEI4064401.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLMRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27372..28175
                     /gene="ybtT"
                     /locus_tag="SI621_001674"
     CDS             27372..28175
                     /gene="ybtT"
                     /locus_tag="SI621_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HEI4064402.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28179..29756
                     /gene="ybtE"
                     /locus_tag="SI621_001675"
     CDS             28179..29756
                     /gene="ybtE"
                     /locus_tag="SI621_001675"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HEI4064403.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFNASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNTQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            29887..>31853
                     /gene="fyuA"
                     /locus_tag="SI621_001676"
     CDS             29887..>31853
                     /gene="fyuA"
                     /locus_tag="SI621_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HEI4064404.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRAFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPRMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVA"
BASE COUNT         6813 a         9595 c         8867 g         6578 t
ORIGIN      
        1 cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact tcagttcccg
       61 ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg tgaccgataa
      121 gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagac ccatagcgaa
      181 tcaagtgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc cttcaagaat
      241 attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata agtcattgat
      301 aaagtggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact ccggttttcg
      361 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
      421 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
      481 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
      541 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acggaacagg aacaaagcaa
      601 attagtcact cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
      661 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
      721 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
      781 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
      841 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
      901 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
      961 cggcgatcgc gccagcggag ttccctttat tccgttgcgt gaggttgctg gctgggaaca
     1021 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattggttg gcgagaatgc
     1081 ggcgagcatc gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     1141 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     1201 ctgccaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     1261 gttttcggcg acgaatgttc attaattaac aactttgcag attaattaac caattgaaat
     1321 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     1381 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     1441 cgagtccagt cagaggagcc aattttctgt tttcttgcat ccttgcgaat ctttatctga
     1501 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     1561 atcgggaaaa attggtggtc aaatctgggg tcaggttagt tcgataatgg agtgaccccc
     1621 atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag
     1681 tctccgattc tcacggtctg tatctgcggg tcaagccggg tggctcccgc cactggtatc
     1741 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     1801 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     1861 acccggtaca gcagcgggct gctgtacgtg gctcacgaac accggagaaa gtttttaaaa
     1921 acgtggcgct ggcgtggcat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     1981 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     2041 ttaaaccccg tcatttcatt gacctgcaga aagggatcga ggaaaaaggt ctgctggagg
     2101 ttgcgtcccg cacacggcag cacctgagta acataatacg ccatgcggtc catcaggagt
     2161 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     2221 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     2281 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     2341 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga ggctggacga
     2401 tacccgcgac gcgagaaccc attattggcg tgcattattc cggccgcggg gcaaaaatgc
     2461 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     2521 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taactcgtat aagccaatgt
     2581 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     2641 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     2701 aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata
     2761 ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc
     2821 tggaagcatg ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc
     2881 catagtatac taacgaatcc cacactctgg caaattacca ccacctccac aggaggtggt
     2941 tctatataga cttttatcaa cttcatgacg aaaaacacct attttatccc aaaagaagac
     3001 aatagagtca cagaggaatc aaagagcgac tcaattgtct ttggacagtc tggttgtgag
     3061 gttagaaaac agttactcct acaccattaa atagggcgca atgctcgcta atttctcccg
     3121 ggtttctgtc agttcgcgtt ccggcgtaga ttgcgcgatg attcccgccc ccgcctgtat
     3181 ccagcagcgc tggctatcct gaaatacgga acgcagaact agcgccgcat caaagcgcgt
     3241 atcgtccagc agcaggattg cgccggaata aagctctcgc ggcgtcttct caatttgcat
     3301 aatcgcgttc agagcagcat ttttagggat acctgaggcg gtaatcgacg gaaacagcac
     3361 ggtaaacgca tcccaggcat ccttgttttc cgcaagctga ccgctcacgc cagaccccag
     3421 atgctgaacg ctgccgcgct ggcgaaccga cattaaatct tcaaccacca cgctgcccgg
     3481 ctggcaaaca gcctccagtt cagcaatagc ttctttgaca gaaaggatat gctcaagcac
     3541 ctctttactg tcgtgcagca gttctgcctc tttcgcctta ttatgctccg ggtttcccat
     3601 gcgatcgcgg gtgcccgcaa gcggttcagt gaccacttta ttgcccgtca ctgacatcac
     3661 cagttccggg ctaaagccca gcgcttcgcg tccttcctga cggaacataa acgagcgcac
     3721 aggcgtgttt gcctgcagcc cgtataacag cgtggcgggc atatcaatcc gcgatggcag
     3781 gggaatggcg cgcgagacaa tcactttgac atactcgcca cggcggatct ccgcaacggc
     3841 gcgcgcaacc tgttgtttat atgcctcacc attgagggcg gtatccaccg ccagtggtgc
     3901 gttctgcgtc gttgtaccgg cctcttttac ccactcgcac aaacgtcggc acccgtcggc
     3961 ggagtccgca taaacggtga catttccctt ttcaaaaata agttcttcac ggggaacggt
     4021 taacgtcagc agcggccact ccccggcgtt aaaggcaatc ccccgcgcat gcgccgcaaa
     4081 gttgaaccca acctgtccat atacccgacg tcctttcacc tgaggatgcg ccataaaacg
     4141 acgcgcgcag tcggcaatgg aatccaccgc ccatttttgc tccccagcat catcaataaa
     4201 tgtcgcctgc gttccgtcgg cattaatgtg cagccgtgcc tggcaccctt tgcctaaata
     4261 ccaacagggt tgacgctcat agacataaca ctcttcttct gcgaactggc gtaaaacgcc
     4321 agaaatcgtc ggtagccatt gttcctctgg taacgccaga tgtagaaatt cactgatttt
     4381 catcccttac ctctctgtgt tattcccgga tcaatcaatt gcgcttgtca tcagttctct
     4441 tgccgaatgc agccggatat aagcaaccca cgccgccagc aacgtgaatg ccccggcaaa
     4501 caggaagcag gccgcatagc caaaatgttg agcaacaacg ccaccgataa cgccagccag
     4561 aatggcgatt gccgcgtcag tacattgaaa gagggtgaag tcgacaccgg cctgcaaagg
     4621 cgatgaaagc gacatcagcg tggcgtaaag acagaccagc gcgcaggcca gcgaaatgga
     4681 ctggacaatg accagacact ggagaatctg cagcagcaga tgacccggcg ccatcatgag
     4741 cgtcatcacc accgccagca gcgcgatccc ctggacgcca taagccgtca acagcgctct
     4801 gccgggtgag gtgtatttca tcagcaatcc gccggccagc gttcctgcta tgcccgctgc
     4861 aatattgccg ccgctgaaca gcgcgcccaa tgcggacatg ctcaacccat gatccaacaa
     4921 cagcggtgcc agaagaggca gcacaaaccg catgcctgaa ttcagcatca ataccagcag
     4981 taagcccagg cgcgcctgct tcctccttag cgcataacct aacgccggaa catgcgggat
     5041 agtcgctgtt cgcgtcggct ccgtaatgcg ccacagcggg agtgacagcg ccataatcag
     5101 caccgccatc atcagcatgg cgacaggcca gccggatgcc gccgacagca tcaggaacac
     5161 cccgccgccg cacatcattc ccagatagct gccgccgacc tgcacgctgt ttccccagcc
     5221 gtaacccgtg cgggtcagtt gatcgacaca aaagccgtcg ctggcgatat caatggtgga
     5281 ggcgaccgtg cctgccacca taaacagaac aaataacgcg acgatttgcg tatcggggaa
     5341 tcccccttct cgcccaaacc agccaatcgc tgcggcaata gtcaggatcg ccgctagcgc
     5401 gacctgacca cgcaggatta acatgcggga acggcgttcc tggctaccgg gcggaagccg
     5461 ccagcgctcg atccacggcg cccaaataaa cttcagcgcc cagggcagca taaacagggt
     5521 tgtcgcaccg gcaagcgcca gcgaaccgcc agcagcgcgc accagcgcgg gtaaggactg
     5581 catagacagc gccgatacca gactctgcgt cacatagacg ccggcaatag caaaaatcac
     5641 ctgcccggtc gtcaacgtca gaggtttcac attcgactga acatcactca tgcacccact
     5701 ctccagacgc ggaacccccg tcgtcgcgcc acacgcgcgc ggccatttgc gcctgccaca
     5761 gcgcctgata acgtccatga tgtgagagca attgcgcatg agtaccctgc tcaaccacct
     5821 gtccctcttc catcaccaga atgtttccgg ccccggcgat ggtggataaa cggtgagcga
     5881 tgataatcac cgtccggttg tgaaccaggt tatcgatcgc tttttgcacc gccagctcgc
     5941 tttcaatatc cagcgcggca gtcggttcat cgagaatgac gaccggcgcg tttttcaata
     6001 acgctctggc aatggaaatc cgctggcgct cgccgcccga tagctggccg cccatctctc
     6061 ccattggcgt cagccagcct tgcggaagac gggaaataaa ctcaaggcac tgcgccgcgc
     6121 gggcggcctc ttctacctcc tgccgcgtcg cctgtgggcg cgcgatacgg atattcgcca
     6181 gcagcgtgtc atcaaacagc cagacatcct ggaaaacaac agaaatcagg ctattgagct
     6241 gttccggcgt caggcggcga atatcgacgc cgccaataga aatctgccct tgctgcggat
     6301 cggcatagcg cattaacagt ttggtgaccg tagttttgcc tgcgccggag gcacccacca
     6361 gcgcgctcat actggctgcc gggaacgtca aagaaacatg attaagcgcg tggccgtcac
     6421 cttcttcgta gcgatagctg acgttgtcaa agcggatatc gtaacgttcc ggcatctcgc
     6481 tttgctctgc gacgggaagc ggtgctatcg ccataaactg ctcaatccgt tgcagggcgc
     6541 tggcgatcag ttccacaacc gaggtgtagc tgataaacat cgccatcggt tctgcgaagc
     6601 gcataatcat cgcgacggcg gcaatcaaaa aggcgaggtt cagagtgccc gtcactaccc
     6661 agacgatccc ggataacacc accacctgta ggcccaactc cacgacgctg gcgatcagca
     6721 tcgttgcgcc agcgccctga cggtgagtgc gggtctgtaa gttttccagc gcattgaaat
     6781 gcgccagcag cgcccggctt ttatcggcat cgctgccgca ggtgcgcaat accatcatcc
     6841 cctgagcaaa ttcaacgata tcgccgctca aacgctggtg cgcttccccc agcgtctgca
     6901 tttgtcgccg catcgccggg cggcgccagt aataaaacgg caccagcaga gggaagatca
     6961 gcaacatcac cagccccagc cgccagtcta tccacaatgt cgccagcgac gccgtcagcg
     7021 gcgtgacaat ggtgagcagc aaaatattgg ctatcgcaat aacataattg aggttttcat
     7081 ccacgctgcc cagcagcaag gcgttcattt cacccgccct gccgcgctgg agcttctcca
     7141 gcggcacgcg gcgtaactgc tcgccaagtc gcaggcgcaa ctcatgggta gcctgcgcca
     7201 gatgaccacg gtattcaaag cccaggccat accagcgtag caccagcgtc acaattgcgg
     7261 cgacgctgaa ggccatagcc cagttaagaa gttgcggcgc gtctccccgt aacagcgcat
     7321 caatgatcgg ataaagacag gcaaaggcga tcccctgcat gaatgccgcc agcaacagcg
     7381 ccagcatact gcgtcgcagc atcctggcct gactgccaac gctggagata agctggcgcc
     7441 agatgacgcg ccaggccagg ttatccgcag gattattgtc tttcattttc caccacctct
     7501 tcctgtgttc cacccggcac ccaatgctgc gcctgctgat agtgttgcca gagccgttga
     7561 tacaggccgc cctgcgccaa cagttgcgta tggttcccca tttccctgag ttgtccgtcg
     7621 gaaaacagca atatcacatc ggcctgagtc accattgaga gacgatgcgc gaccatgatg
     7681 accgtccggc cacgcatggc cgccgcgagc gccttgataa gcgccgcttc gttttccggg
     7741 tcagcaaacg ccgtcgcctc atcgagcacc aggatggggc gatcctgcaa aagcgcccgg
     7801 gcgatagtaa tgcgctgccg ctggccgccg gagagaaata ccccacgttc cccgactcga
     7861 gtgttgtaac cttctggcag agcgctaata aaatcatggg cctgcgccac tctggccgcc
     7921 gctataaccg cctccagcgg cgtatccggc gcgcccagac gaatgttatt ggctatcgtg
     7981 tcggcaaaaa ggaagttgtc ctgaaacaca aacgagagtt gcttcatcag ggtgtccgtc
     8041 tgcatatcac gcagatccac gccgccaata cggatatggc ctttgtccgg gtcggcgtaa
     8101 cgcagcagca agcgcgccac ggtgcttttt ccggcgccgc ttggcccgac cagcgccaca
     8161 atttgcccgg caggcacatg aaagctcacc tcctgcaacg cggcgccagt acgcgcttgc
     8221 ggataatgaa agctcacctg ctcaaaggta atgctcgcct cttgcggctg ctggtcagac
     8281 tgcggcagcg ataactccgg catcgcgaga acctgataaa tacgctgaat gcttaaacgc
     8341 gtttgcgcga ccaggttatt gagcatcatc attggcatta cggcttcggc cattccgctg
     8401 cccagcagta aaacggccac ccacgcgata aaatcgaaac tgccatagtg caacaggccg
     8461 tatcccgacc agatcaggac aaacagggtc gggagaggat tcagaatcga gaaggaaaaa
     8521 cgcgctgaaa aaccggcttt gcgataccag gttttgagca catcgaccca ctcttcaagg
     8581 gcgcgttgat agcgcaaaaa actggtgctg ccgctatcaa acgttctcac cacgggcatg
     8641 gcctgcacga actcaataac cgccgcgcta acctgctcgc gcgccttatg ataacgctga
     8701 gccatatttt ccgaacgctg gcgggcgagc acgagaacaa ccgatccaaa cgccagtacc
     8761 cccagcgtcg cgattgccag ccgccagtcc agccagaaca agataacgat tgtcgccagc
     8821 ggcatgatga tcgcgcgggc atagagcggc gtgctgtcgg ccacaaaaat atgtaacgac
     8881 ttcacgtcat ccagcatcac tttcgccact gacccgctgc ccatttgctg taacacgcca
     8941 ggcggaagct gcaatgcttt gcgggctaac ctgctgcgca gaatgttctc cagatgaaaa
     9001 gccgcgtaat gagaggtgtt gaacgcctgt aagcgcagaa caaaagttaa gaccacgcag
     9061 ccgatcacgc cgcccagcgg ccaggccgga atagcgtcag gcgtcgcgcg aatgtcacgc
     9121 aggctccacg ccagaaaaag gagcgccccc aggctggtta atgccgccag ccccgccagc
     9181 gccatagcta aaataacctt cctgcgaacc ggtgtaatca cctgccagag aggaaatcgg
     9241 gacagcgatt cagtattgga tgattgagat gacatgggag taactgaatt tcctgatgaa
     9301 tttttacgga cgccaaaaac gacaattcag ggtggcgttc tgagaattaa tgtgaataat
     9361 aaccattatc aataactcat ctaccccatt cggttctatt tatacccgca ttggtctaag
     9421 ccacagggag ataaccaggt catgacggag tcaccgcaaa cgcaatctga aatctctatt
     9481 caccagttgg tggtcgggaa acctgcaaac gatggcaata ttcctgctca gtgtgaactg
     9541 ctgcgctgtt cgttgcagga aggtatggat attttgctct ggaggggcca ttttgcgcgg
     9601 ccagagacgt tgcagctaca cgacgatctg ggcaggatta acttctcgtg catcctggaa
     9661 ggcacatcgc gctttgcgat tcagggactc cggcgccaca ccgactggga gcttgcgcgc
     9721 aatcgccatt acattaccca tacgccagac tgtcgcggca gcgcctccta ttgtggccga
     9781 ttcgagagca ttaccctctc cttcagccct gaaaccctcg cgctgtgggt gccggatatt
     9841 agcgcggtca ttaagaataa gatcgactcc cactgctgtt gccagcagca gcgttgtaat
     9901 gctgaaaccc acttaacggc tcaggccttg cgtcacgcgc tgatgcgcat gcacggcggc
     9961 ttcagtcatg aacaaaagcc gtcgacgcta tggttgcagg ggcaaagtct ggtcatgctc
    10021 agcctggtgc tggatgaaca tcgcgaagac gccagttgcc tctcctgcca cttcaacccg
    10081 atggaacgcc agaaactgct gcgggcgaag gatctgctgc tggctgatct gacgcaagcg
    10141 ccgggcgtcg cggagctggc cagagagtcc ggtctgagcg ttctcaaaat taagcgaggc
    10201 ttccgcgttc tgttcaacaa cagcgtctat ggcctgtttc aggctgaacg aatgcaggaa
    10261 gccagaagac gcctggccaa cggcaatacc tccgtcatga cggtggcggc cgatctgggc
    10321 tatgccaacg ccagccattt ctctgccgcc ttccagaaac agtttggcgt aactccttcg
    10381 acctttaaac gcgggatgta accggggtcg cgccccccta aactcctccc tgacagaggc
    10441 ccggatacct catccgggcc tctgttcttt ccgcctgtta ccaacccgaa acgggcataa
    10501 ataaacccgt ttcgggtagc attcaattgt taataattat tattctcata tgagtaatgc
    10561 ttttcggtaa gacgtgccat caggaggaag aatgatttct ggcgcaccat ctcaggattc
    10621 gctgttaccg gacaaccgcc acgcggctga ttaccaacaa ttacgcgagc ggctcataca
    10681 ggaactgaat ttaacgccgc agcagttaca tgaagagagc aacctgatcc aggccggcct
    10741 ggattccata agattgatga gatggttaca ctggtttcgt aaaaatggct accgccttac
    10801 ccttcgcgag ctgtatgccg cccccacgct ggcggcatgg aaccagttaa tgctcagccg
    10861 gtcgccggag aacgcggaag aagaaacgcc gcccgacgaa tcatcctggc cgaacatgac
    10921 cgaaagtacg cccttcccat tgacgccagt acagcacgcc tacctgacgg gccgcatgcc
    10981 ggggcagacg cttggcggcg tgggttgcca cctgtatcag gagtttgaag gccattgtct
    11041 gacggcgtcg cagctggagc aggccatcac gaccttgctg caacgccacc caatgctgca
    11101 tatcgccttt cgccccgacg ggcagcaggt ctggctaccg caaccttact ggaacggcgt
    11161 caccgttcat gatttacgcc ataacgacgc tgaaagccgc caggcctatc tggacgcact
    11221 gcgccagcgc ctgagccacc gtcttttacg cgtggaaatc ggcgaaacgt ttgattttca
    11281 gctgacgctc ttgccggaca atcgccaccg cctccatgtc aatattgacc tgctgattat
    11341 ggatgcctcc agctttacgc ttttcttcga tgagcttaac gccctgctgg ccggagaatc
    11401 gctgccggct atcgacaccc gctatgattt ccgctcgtat ttgctgcacc agcagaagat
    11461 caatcaacca ctgagagacg acgcgcgcgc ttactggctg gcgaaagcat cgacgcttcc
    11521 ccccgcgccc gtcttgccgc tggcctgcga acccgccacg ctacgtgaag tccgtaatac
    11581 ccgacgccgc atgattgtcc cggcaacacg ctggcacgcc tttagcaacc gggccggcga
    11641 gtatggcgtg acgccgacaa tggcactggc gacctgtttt tctgccgtgc tggctcgctg
    11701 gggcggcctg acgcgtctgc tgcttaacat caccttattc gaccgccagc cgctgcaccc
    11761 ggcggttggc gcgatgcttg ccgacttcac caatattctt ctgctggata ccgcctgcga
    11821 tggcgatacc gtcagcaacc tggcgcgtaa aaaccagctc acgtttacgg aggactggga
    11881 gcatcgccac tggtccggcg tcgaattact ccgtgaactc aaacgccagc agcgctaccc
    11941 ccacggcgcc ccggtggtat ttaccagcaa tctggggcgt tccctctaca gcagccgcgc
    12001 agaatcgccg ttgggcgagc cggaatgggg catctcgcaa acgccgcagg tctggataga
    12061 tcatctggcg ttcgagcatc acggcgaggt ctggctacaa tgggacagca acgacgcgct
    12121 gttccctccg gcgttagtcg aaacattgtt cgacgcctac tgccagttga ttaaccaact
    12181 ctgcgatgac gaaagcgcct ggcaaaagcc gttcgcagat atgatgcccg ccagccagcg
    12241 cgcgatacgc gaacgggtca acgccaccgg cgcccccatt cccgaaggct tgctgcatga
    12301 aggcattttc cgtatcgctc tgcaacagcc gcaggcgctg gcggtaacgg acatgcgtta
    12361 tcagtggaat tatcatgagc tgacagacta tgcccgccgt tgcgcgggca ggttaatcga
    12421 gtgcggggtt cagcccggcg ataatgtggc tatcacgatg tcgaaaggcg caggacaact
    12481 tgttgcggtt ctggccgtcc tgctggccgg ggcggtttac gttccggttt cgctggatca
    12541 gcctgccgca cggcgcgaga aaatctacgc tgacgccagc gtccggctgg tgctcatttg
    12601 tcagcacgac gccagcgccg ggtcagacga tattcccgtc cttgcctggc agcaggccat
    12661 tgaggcggag ccgatcgcca acccggtagt acgcgccccc acgcaaccgg cctacattat
    12721 ctacacctcc ggctctaccg gtacgccgaa aggggtagtc atttctcacc ggggagcgct
    12781 taacacctgt tgcgatatca atacccgcta tcaggttggc ccgcatgaca gggtgctggc
    12841 cctctccgcc ctacattttg atttatcggt ttacgacatt tttggcgtac tgcgcgcggg
    12901 cggcgcgctg gtgatggtga tggaaaatca acggcgcgat cctcacgcat ggtgtgagct
    12961 gatccagcgc catcaggtca cgctctggaa cagcgtcccg gcgctgttcg atatgctgct
    13021 gacctggtgt gaaggtttcg ccgacgccac gccggaaaac ctgcgcgcag tgatgctttc
    13081 cggcgactgg atcgggcttg acctccccgc ccgttatcgg gccttccggc cacaaggaca
    13141 atttatcgcg atgggcggcg ccaccgaggc gtctatctgg tctaacgcct gcgaaattca
    13201 cgacgtcccc gcccactggc gctccatccc ttacggtttt ccgctaacca accaacgcta
    13261 ccgggtggtg gatgaacagg gccgggactg ccctgactgg gtgccgggtg aattatggat
    13321 tggcggcatt ggggtcgcgg aaggctattt caacgatccc ctgcgtagcg agcagcaatt
    13381 tttgacgctc ccggacgagc gctggtatcg caccggcgat ctcggctgct actggccaga
    13441 tggcacaatc gagttcctcg gtcgtcgcga caagcaggtc aaagtcggag gatatcgcat
    13501 cgagctgggc gaaatcgaaa gcgcgctcag ccagctggcg ggggtgaaac aagcaaccgt
    13561 tctggcgatc ggcgaaaaag aaaaaacgct ggcggcatac gttgttcctc agggcgaggc
    13621 tttttgcgtt accgatcatc ggaacccggc actgccgcag gcgtggcaca cgcttgcggg
    13681 aacgttgccc tgttgcgcca tctcgccaga gatctccgca gaacaggtag ccgatttcct
    13741 tcagcatcgc ctgctaaaac tgaagccggg tcacaccgct ggcgccgatc ctctccccct
    13801 gatgaactca ctcgctatcc agcagcgctg gcaggccgtg gtggaacgct ggttagcatt
    13861 tctggtgaca caacggcgac tgaagcccgc tgctgaaggt tatcaggtct gcgctggtga
    13921 agaacgcgag gatgagcacc cgcacttcag cggacatgat ttaacgttat cgcaaattct
    13981 tcgcggtgcc cgtaacgaac tgtcgttact gaacgacgcg cagtggtcgc cggaaagcct
    14041 ggcctttaac catccggcca gcgccccgta tattcaggaa ctggcgacaa tttgccaaca
    14101 gcttgcacag cgcttacagc gcccggtacg cctgcttgag gtgggaaccc gcactggccg
    14161 cgccgcagaa tcgctgttag cacagctcaa cgccggacag attgagtatg tcgggcttga
    14221 gcagagccag gagatgctgc tgagcgcccg gcagaggctc gccccctggc ctggcgcccg
    14281 tctgtccctc tggaatgcag acacgctggc gacgcacgct cactcggcgg acattatctg
    14341 gcttaataac gccctgcatc gtctgctgcc ggaagatccc gggctccttg cgacattaca
    14401 acagcttgcc gttcccggcg cgctgctcta cgtgatggag tttcgccagt taacgccgtc
    14461 cgccctactc agcacgctcc tgttaaccaa tgggcagccg gaggccttgc tgcataacag
    14521 cgccgactgg gcggcattat ttagcgcggc cggcttcaac tgtcagcatg gcgatgaggt
    14581 cgcggggtta caacgcttcc tcgtacaatg tcctgacagg caggtgcgcc gcgatccccg
    14641 tcaacttcag gccgccctcg ccgggcgtct gccggggtgg atggtgccgc aacggatcgt
    14701 attcctcgac gccttaccgc tgacggctaa cgggaaaatt gactaccagg cgctgaagcg
    14761 tcgtcatacc cctgaagcgg aaaacccggc cgaagcggat ttaccccagg gcgacattga
    14821 aaaacaggtt gccgccctct ggcagcaact cttatcaact ggcaatgtca ccagagaaac
    14881 cgacttcttc cagcaaggcg gcgatagcct gctggcgacc cgtctgaccg ggcaacttca
    14941 tcaggcaggt tatgaagcgc aattaagcga cctgtttaat catccccggc tggcggattt
    15001 tgccgccacg ctgcggaaaa ccgacgtccc ggtcgaacaa ccattcgtcc actcccctga
    15061 agatcgctac cagccctttg cgcttaccga cgtgcagcag gcttacctgg tggggcgtca
    15121 gccgggcttt gccctgggcg gcgtcggctc acatttcttt gttgaatttg aaattgccga
    15181 tctggacctc acccggctgg agacggtctg gaaccgatta atcgcccgcc acgatatgct
    15241 gcgcgccatc gtgcgtgatg gacagcaaca ggtgctcgaa cagacgcccc cttgggtgat
    15301 acccgcacac accctccata cgcctgaaga ggcgttgcgg gtgcgcgaaa aactggcgca
    15361 tcaggtactc aaccccgaag tgtggccggt attcgatctc caggtcggat acgtggacgg
    15421 gatgcctgcc cgcctgtggc tgtgtctgga taacctgttg cttgacggtc tgagcatgca
    15481 gatcctgctg gcggagctgg agcacggcta ccgctacccg caacagctgc ttccgccgct
    15541 gcccgtcacc ttcagggatt atctgcaaca accctcgcta cagtcgccca atccagattc
    15601 tctggcatgg tggcaggcgc agcttgatga tattcctccg gcgcctgcgt tgccgctgcg
    15661 ctgcttgcct caggaggttg aaacaccgcg cttcgcccgc ctgaacggcg cactggacag
    15721 cacgcgctgg catcggctga aaaaacgggc ggctgacgcc catctcaccc cgtcggccgt
    15781 actgttgtcg gtgtggtcaa cggttctctc tgcatggagt gcacagcctg agttcacgct
    15841 taaccttacg cttttcgaca ggcgaccgct gcacccgcaa atcaaccaga ttctgggcga
    15901 tttcacctcg ctgatgctgc tgagctggca tcccggcgaa agctggctgc acagcgcgca
    15961 gtcactacag cagcggctga gccagaacct caaccaccgc gatgtgtcag ccatccgcgt
    16021 gatgcgtcaa ctggcgcaac ggcaaaacgt gcctgccgtt ccgatgcccg tcgtctttac
    16081 cagcgcactg ggctttgagc aggataactt cctcgcccgg cgtaatctgc tcaaaccggt
    16141 ctggggcatc tcccagacgc cgcaggtctg gctcgatcac cagatttatg aatccgaagg
    16201 cgaactgcgc tttaactggg attttgtcgc cgcgctgttt cctgccgggc aggtggagcg
    16261 ccagtttgaa cagtattgcg cattgctaaa ccgaatggcc gaggatgaaa gcggctggca
    16321 actgccgctc gccgcgctgg tgcctcccgt taaacacgca gggcaatgcg cagagcgctc
    16381 accgcgcgta tgccctgagc actctcagcc acacattgcg gcggacgaga gcaccgtcag
    16441 cctgatttgc gacgccttcc gcgaggtggt tggcgagtct gtcacgcccg cagaaaactt
    16501 ctttgaggcg ggcgcaacgt cgctgaatct ggtgcaactg cacgttttgt tacaacgtca
    16561 cgaattttcc accctgacgt tgcttgacct cttcacccac ccttctcctg ctgccctggc
    16621 cgattatctg gccggcgtcg ccacggtgga gaaaacaaaa cgacctcgcc ctgttcgccg
    16681 tcgtcagcgg cggatatagc gcgaagcaaa ctgattttcc ccggaacgcc atcgcgaacg
    16741 catggcgttc cattgacttt gtgaatctta ggaaacggga ccgattatgg ataacttgcg
    16801 cttctcttct gcgccgacag cagattccat tgatgcatcg atcgctcaac actacccgga
    16861 ctgcgaacct gtcgcggtta tcggctacgc ctgccatttt cctgaatcgc cggatggcga
    16921 aacgttctgg caaaatctgc tggaaggtcg tgaatgcagc cgacgcttta cgcgcgaaga
    16981 gcttctggcc gtcggtctgg atgccgccat cattgacgat cctcattatg tcaatatcgg
    17041 tacggtgtta gacaacgccg actgcttcga cgccaccctg tttggctatt cgcgacagga
    17101 agcggagtcg atggacccgc agcagcgcct gtttttgcag gcggtctggc atgcgctgga
    17161 acatgccggt tatgcccccg gcgccgtccc ccataagacc ggcgttttcg cctcttcccg
    17221 gatgagtacc taccccggtc gcgaagcatt gaacgtgaca gaagtcgcgc aggtaaaagg
    17281 tctgcaatct ctgatgggca atgataaaga ctatattgcc acccgcgccg cgtacaaact
    17341 caacctgcac ggcccggcgt tatcggtaca gaccgcctgc tccagctcgc tggttgccgt
    17401 gcatctggcc tgtgaaagcc tgcgcgcagg cgaatccgat atggcggtgg ccggcggcgt
    17461 ggcgctctct ttcccccagc aggcgggcta tcgctaccag cccggaatga ttttctctcc
    17521 cgatggccac tgtcgtcctt ttgacgcctc ggctgagggc acctgggccg gtaatggtct
    17581 cggctgcgtg gttctgcgtc gcctgaaaga cgcgctactg tcaggcgatc cgattatctc
    17641 ggtgatcctc tccagcgcgg tcaataacga cggcaacaga aaggtcggct ataccgcccc
    17701 ttccgtcgcc gggcagcagg cggtcatcga agaggcgtta atgctggcgg ccatcgacga
    17761 caggcaggta ggttacattg aaacccacgg caccggcaca ccgctgggcg atgcgattga
    17821 aatagaagcg ttacgcaacg tctatgcgcc gcgcccgcag gacaggcgct gtgcgctcgg
    17881 ttccgtgaaa agcaatatgg ggcatctgga taccgcggca ggcattgccg gactgctgaa
    17941 aaccgttctg gccgtcagcc gcggacaaat tccaccctta ttgaatttcc acacccccaa
    18001 cccggcgctg aagcttgagg agagcccctt taccgtacca gtgtcggcgc aagcgtggca
    18061 ggatgaaatg cgctatgcgg gcgtatcctc ctttggtatt ggcggcacca actgccatat
    18121 gatcgtcgcc tcgctgcccg atgcgctcaa cgcgcgcctc cccaatacgg atggcggcag
    18181 aaaaagtact gcgctgctgc tcagcgccgc cagcgacagc gcgttgcggc ggctggcgac
    18241 ggattatgcc ggggcgctga ggaagaatgc ggatgccagc gatctggcct tcacggccct
    18301 gcacgcgcgc cgtctcgatc ttcctttccg cctggcggtg ccattaaacc gtgaaaccgc
    18361 cgacgcgctc agcacctggg ctagtgagaa atcgggggcg caggtataca gcggccacgg
    18421 cgccagcggc aagcaggtgt ggctgtttac cggccagggc tcgcactggc gcactatggg
    18481 ccaaacgatg taccagcact caacggcgtt tgccgacacg ctggatcgct gtttttccgc
    18541 ctgtagcgaa atgctcacgc cgtcactgcg cgaagcgatg tttaaccccg attcggcgca
    18601 gctggacaat atggcctggg cgcagccggc gattgtcgcg tttgaaatcg cgatggcggc
    18661 gcactggcgt gctgaaggac tgaagccaga cttcgccatt gggcattccg tcggtgaatt
    18721 tgccgctgcc gttgtctgcg gacactatac gattgaacag gtcatgccac tggtttgtcg
    18781 gcgcggcgcg ctaatgcagc agtgcgcaag cggcgcgatg gtggcggtat ttgcagacga
    18841 agacacgctg atgccgctgg ctcgccagtt tgagctggat ctcgccgcca acaacggtac
    18901 gcaacatacg gtattttccg ggccggaagc ccgtctcgcg gtattttgcg ccacgctctc
    18961 gcagcatgac attaactatc gtcgcctgag cgtaaccggt gcggcgcact ccgctttact
    19021 ggagccgata ctcgatcggt tccaggacgc ctgcgcggga ctgcacgcgg agccggggca
    19081 aataccgatt atttccacgc tcaccgccga cgtcattgat gagtcaacgc tcaaccaggc
    19141 ggattactgg cgccgacaca tgcgccagcc ggtgcgtttt atccagagta ttcaggtggc
    19201 gcatcagctc ggcgcccgcg tttttctgga gatggggccc gatgcccagt tggttgcttg
    19261 cgggcagcgc gaataccgcg ataacgcata ctggatagcc agcgcccggc gtaacaaaga
    19321 ggcgagcgat gtcctcaatc aggccctgct ccaactttac gctgccggcg tcgccctacc
    19381 gtgggccgac ctgctggcgg gcgatggaca acgtatcgct gcgccatgtt atccgtttga
    19441 tactgaacgt tactggaaag agcgcgtctc cccggcctgc gagcctgccg acgcagcgct
    19501 gtctgccggg ctggaggtgg cgagtcgcgc cgcgacagcg ctcgatctcc ctcgcctgga
    19561 agcgcttaaa cagtgcgcca cgcgactgca cgccatctac gtcgatcaac tggtacaacg
    19621 ctgtaccggc gatgccattg agaacggcgt ggacgccatg accatcatgc gccgtggacg
    19681 tctgctgccc cgctaccagc agctactcca gcgtctgctg aataactgcg tggtcgacgg
    19741 cgattaccgc tgcaccgacg ggcgatacgt ccgcgcccgc cccattgaac atcaacagcg
    19801 ggaatcactg ctgacggaac ttgccggtta ttgtgaaggt tttcaggcta ttcccgacac
    19861 catcgcccgt gccggcgatc ggttatatga aatgatgagc ggcgcggaag aaccggtggc
    19921 gattatcttc ccgcaaagcg cctccgacgg cgtggaagtg ctgtatcagg aattcagctt
    19981 tggccgctat ttcaaccaaa tcgccgccgg ggtattacgc ggcattgtcc agacgcgtca
    20041 gccccgccag ccgttgcgta ttcttgaagt tggcggcgga accggcggca ccaccgcgtg
    20101 gctgctgccg gaactcaacg gcgttccggc actggagtac catttcaccg atatctcggc
    20161 gctgttcacc cgtcgcgccc agcagaaatt cgccgactat gattttgtga agtatagcga
    20221 gctggatctc gaaaaagagg cgcagtctca gggtttccag gcacagtctt acgatcttat
    20281 cgtggcagcg aacgtgattc acgccacccg ccatattggc cgcacgctcg ataatctgcg
    20341 ccccctgctc aagccgggcg ggcgcctgct gatgcgcgaa atcacccagc caatgcgtct
    20401 gtttgacttc gttttcggcc cgctggttct tccgctacag gatctcgacg cccgcgaagg
    20461 tgagttattc ctcaccaccg ctcagtggca acaacagtgc cgccacgccg gattcagcaa
    20521 agtggcgtgg ctaccgcagg atggcagccc gaccgccggg atgagcgaac atatcattct
    20581 cgccacgctg cccggtcagg cggttagcgc cgtaacattc accgcgccat cagaacccgt
    20641 gttggggcag gcgctgacgg ataacggtga ttatctcgcc gactggtctg attgcgcagg
    20701 tcagcccgaa cggtttaacg cccgctggca ggaggcctgg cgtctgcttt cacagcgtca
    20761 tggcgacgct ctgcctgtgg aaccgccccc cgtcgccgcc ccggagtggc tagggaaggt
    20821 tcgcttaagc tggcaaaacg aagccttttc ccgcggtcag atgcgcgttg aagcccgtca
    20881 tcctgctggc gagtggctgc cgctatcgcc cgccgcgcct cttcctgcgc cgcaaacgca
    20941 ttatcaatgg cgctggacgc ccctcaacgt cgccagcatt gaccatccgc ttacctttag
    21001 cttcagcgcc ggtacgcttg cgcgcagcga cgagctggcg caatacggca tcattcacga
    21061 tccgcacgcc tcttcacgac tgatgattgt tgaggagagc gaggatacgc tggccttagc
    21121 ggagaaagtg atagcagcgc tcaccgccag cgcagccgga ttgattgtgg ttactcgccg
    21181 cgcgtggcga gtcgaggaaa atgaagcact ctctgcatcc catcaagcgc tatgggcctt
    21241 gcttcgcgtc gcggccaacg aacagccgga acggttgctt gccgccatcg atctcgccga
    21301 aaacaccccg agggaaacgc tgcatcaagg gttgagtgca gtctcactat cacagcgctg
    21361 gctcgccgca cggggtgaca ccctttggct tccttcactg tcgcccaata cgggatgcgc
    21421 cgctgaatta ccggcaaacg tgtttaccgg cgatagccgc tggcatctgg tgaccggagc
    21481 gtttggcgga ttaggccgcc ttgccgtgaa ctggctcaga gaaaaagggg cgcgacgcat
    21541 cgccctgctg gcgccgcgcg tggatgagtc atggctacgc gacgtggagg gcgggcagac
    21601 gcgcgtctgc cgttgtgatg tgggcgatgc cgggcaactg gccacggttc ttgacgatct
    21661 ggcggccaac ggcggcattg ccggagcgat tcatgccgct ggcgtattgg ctgacgcgcc
    21721 cttgcaggag cttgatgacc accagctggc tgccgttttc gcggtaaaag cgcaggcggc
    21781 aagccagctg ttgcaaaccc tgcgcaacca cgacggacgc tatcttattc tctactcttc
    21841 cgctgccgcc accctcggcg cgccgggtca gagcgcccat gcgctggcct gcggctacct
    21901 ggacgggctg gcccagcagt tttccaccct tgatgcgccg aaaacgctct ctgtcgcctg
    21961 gggcgcatgg ggagaaagcg gtcgggcggc cacgccggaa atgctggcga cgctcgccag
    22021 ccgaggtatg ggcgcgttaa gcgatgccga aggctgctgg cacctggaac aggcggtgat
    22081 gcgcggcgcc ccgtggcgac tggcgatgcg cgtttttacc gacaaaatgc ccccgttaca
    22141 acaggctctg tttaacatca gcgccacaga aaaagccgca acgccggtca ttcctcctgc
    22201 tgatgacaac gcctttaacg gcagcctgag cgatgaaaca gcggtgatgg catggctgaa
    22261 aaagcggatt gcggttcagc taaggctgag cgatccggcg tcactgcatc caaaccagga
    22321 tctgttgcaa ctcggcatgg actcgctgct cttccttgaa ctcagtagcg atattcagca
    22381 ctacctgggc gtacgcatca atgcggaacg ggcgtggcag gatctgtctc ctcatggact
    22441 cacgcagctt atctgttcta agccagaggc gacgcctgcc gcttcgcagc cggaagtgtt
    22501 gcggcacgac gccgacgagc gttatgcgcc cttccctttg acgcccattc agcacgccta
    22561 ctggctgggg cgaacccacc tcattggcta tggcggcgtc gcctgtcacg tcctgtttga
    22621 gtgggataaa cgccacgatg agttcgatct cgccatactg gagaaagcat ggaaccagct
    22681 catcgcacgc cacgatatgt tgcgtatggt ggttgatgcc gacgggcagc agcgaatcct
    22741 ggcgacaacg ccggagtatc acatcccgcg tgacgatctg cgcgcgcttt ccccggaaga
    22801 acagcgcatc gcgctggaaa aacggcggca tgaactgagc tatcgcgttt tgcctgccga
    22861 ccagtggcct ctttttgagc tggtggtcag cgaaatcgac gattgccatt accgtctgca
    22921 tatgaacctc gaccttttgc agtttgatgt gcagagtttt aaagtcatga tggacgacct
    22981 ggcgcaggtc tggcgcggtg aaacgctggc gccgctcgct attaccttcc gtgattatgt
    23041 gatggctgaa caggcgcgcc gacagacatc ggcatggcac gatgcctggg attactggca
    23101 ggaaaaactg ccgcaactgc ccttagcgcc agagctgccg gtggttgaga cgcccccgga
    23161 aacgccacac ttcaccacct tcaaatcgac gatcggcaag acagaatggc aggccgtgaa
    23221 acagcgctgg cagcagcaag gcgtcacacc gtctgccgcg ctgctcacgc tgtttgccgc
    23281 cacccttgag cgctggagcc gtaccacaac atttacgctg aacctgacgt tcttcaatcg
    23341 ccagccgatc catccgcaaa tcaaccagtt gattggtgat tttacctccg tcacgctggt
    23401 tgattttaac ttctcagcgc cggtgacgtt gcaagagcag atgcaacaga cccaacagcg
    23461 cctctggcaa aacatggcgc acagtgaaat gaacggtgtt gaggtgatcc gtgagctggg
    23521 ccgcctgcgc ggatcacaac gtcaaccgct gatgccggta gtgtttacca gtatgctggg
    23581 gatgacgctg gaaggcatga ctatcgatca ggcgatgagc catctgttcg gcgaaccctg
    23641 ctatgtattc acgcaaacgc cgcaggtctg gctggatcat caggtcatgg agagcgacgg
    23701 cgagttgatg tttagctggt actgcatgga caacgtgctg gaacccggcg ctgccgaggc
    23761 gatgtttaat gactattgcg ccatcctgca agccgtcatc gccgcccctg aaagcctgaa
    23821 gactctcgcc agcggcatcg ccgggcacat tccccgccga cgctggccgc tgaacgcgca
    23881 ggcggactac gacctgcggg atattgagca ggcgacgctc gaataccccg gcatccggca
    23941 ggccagagcg gaaataaccg aacagggcgc gttgacgctg gatatcgtga tggccgacga
    24001 tccgtcgcca tcagcggcga tgcctgatga gcacgaactt acccaactgg cgctgccgtt
    24061 gcctgagcag gcgcagcttg atgagctgga ggcgacctgg cgctggctgg aggcgcgtgc
    24121 gctacagggg atcgcggcta cgctaaatcg tcacggcctg tttaccacgc cggagatcgc
    24181 ccatcgcttt agcgcagtag tacaggcgct gtccgcgcaa gcgtctcacc agcgtctgct
    24241 gcgccagtgg ctacagtgtc tgacggaaag agagtggtta atccgcgaag gtgaaagctg
    24301 gcgctgccgc attccgctca gcgagattcc tgagcctcag gaagcgtgcc cgcaaagcca
    24361 atggagccag gcgctggcgc agtatctgga aacctgcatc gcccggcacg acgccctctt
    24421 ctccgggcag tgttctccgc tggaattgct gttcaacgag cagcatcgcg ttaccgacgc
    24481 gctgtatcgc gacaaccccg ccagcgcctg tctgaatcgc tataccgcgc agattgccgc
    24541 cttgtgcagc gcagaacgga ttctggaggt tggcgccgga accgcagcca ctaccgcgcc
    24601 ggtgctgaag gccacgcgga acacgcggca gtcgtaccac ttcacggacg tctccgcgca
    24661 gttcctcaat gacgccagag cccgtttcca tgatgaatcg caggtgtctt atgccttgtt
    24721 cgacatcaac cagccgctgg atttcaccgc ccacccggag gcgggttacg acctgatcgt
    24781 tgccgtcaat gtgctccacg acgccagcca tgtcgtccag acgttgcgca gattaaaact
    24841 gttgctgaaa gccggcggac gtttgctgat cgttgaagcg acggagcgaa acagcgtatt
    24901 ccagctggcg agcgtgggct ttattgaggg attaagcgga taccgcgatt tccgccgccg
    24961 ggatgagaaa ccgatgctca cccgctccgc atggcaggag gttctcgttc aggccgggtt
    25021 tgcaaacgag ctggcgtggc ccgcgcagga atcgtcgccg ctgcgccagc atctgctggt
    25081 agcgcgttcg cctggcgtaa atcgcccgga taaaaaagcc gtgagccgct atttacagca
    25141 gcgctttggc accggtctgc ccattttaca gatccggcaa agagaagcgt tatttacgcc
    25201 gctgcatgcc ccgtctgatg cgccgactga gccagccaaa cccacgccag ttgccggggg
    25261 gaattcggcg ctggaaaaac aggtggctga actctggcaa tcgctgctgt ctcgccccgt
    25321 ggcaaggcat cacgactttt tcgaactggg cggcgacagc ctgatggcga caaggatggt
    25381 cgcgcagcta aaccggagag ggattgccag ggctaacctt caggatctgt tcaaccattc
    25441 gacgctgagc gacttctgcg cccatctaca ggcggctacg tcaggagagg acaacccgat
    25501 acccctttgc cagggcgacg gtgaggaaac cctgtttgtc ttccacgctt cagacggcga
    25561 tatcagcgcc tggctgccgc tcgctaacgc gttgaacagg cgcgttttcg gcctgcaagc
    25621 aaaatcgccg cagcgctttg ccacgctcga ccagatgatc gatgagtatg tcgggtgcat
    25681 ccgtcgtcag cagcctcacg gcccttatgt gctggcgggt tggtcgtatg gcgcgtttct
    25741 tgcggcgggc gccgcacagc gcctgtacgc caaaggcaag caggttcgga tggtgttaat
    25801 cgatcccgtg tgccgacagg atttctgttg cgaaaaccgg gcggccctgc tgcgcctgtt
    25861 agccgaagga caaacgcctc tggcactgcc cgaacatttc gaccagcaga cgcccgacag
    25921 ccagcttgcc gactttatca gcctcgctaa aacggtcggt atggtgtcgc aaaacctgac
    25981 gctgcaagcg gcagaaacgt ggctcgacaa catcgcgcat ctgctgcgtt tactgactga
    26041 gcatacgccg ggcgaaagcg ttccggtccc ctgtctcatg gtgtatgccg ccgggagacc
    26101 cgcgcgctgg acgccagcag aaaccgagtg gcagggctgg ataaacaacg ccgacgacgc
    26161 tgtgattgaa gccagccact ggcaaatcat gatggaagcc ccccacgttc aggcttgtgc
    26221 gcaacacatt acgcgctggc tttgcgcaac ctcaacgcaa ccggagaaca cgttatgatg
    26281 ccgtccgcct ccccaaagca acgcgtactg attgtgggcg ccaaatttgg cgaaatgtac
    26341 ctgaatgcct ttatgcagcc cccggagggg ctggaactgg tcggcctgct ggcgcaggga
    26401 agcgcccgtt caagagagct ggctcatgcg tttggcattc cgctgtatac ctcgccggaa
    26461 cagataacca ggatgccgga tatcgcctgt attgtcgtgc gttcgaccgt cgccggagga
    26521 accggtacgc agcttgccag acactttctg acgcgcggcg tacacgtcat tcaggagcat
    26581 cccctccacc cggatgacat cagttcctta caaacgctgg cgcaggaaca gggctgctgc
    26641 tattgggtaa acacgtttta tccgcatacg cgcgcgggac gcacatggct tcgcgatgcg
    26701 cagcagctgc gtcgctgcct ggctaagacg ccgccggtag tccacgccac caccagccga
    26761 cagttgctct actccactct ggatttgttg ttgctggctc tgggcgttga tgccgccgcc
    26821 gtggagtgcg acgtggttgg cagctttagc gattttcact gtctgagact cttctggccg
    26881 gagggagaag cctgcctgct gcttcagcgc tatctcgatc ctgacgatcc agatatgcat
    26941 agccttatca tgcaccgcct gctgctgggc tggccggaag gtcatctttc gctggaggcg
    27001 agctacggcc ccgtcatctg gtcatccagc ctgtttgtcg cagaccatca ggagaacgcc
    27061 cacagcctct accgcagacc ggagatcctg cgcgatctgc cgggtctgat gcgcagcgcg
    27121 gcgcccctga gctggcgcga ctgctgcgaa accgtcgggc cggagggcgt cagctggttg
    27181 ctacaccagt tgcgcagcaa tctggcgggc gaacatccac cagcggcctg ccaaagcgta
    27241 catcagatcg cgttatcacg tttatggcag cagattttac gtaagaccgg caatgcggag
    27301 atcagacgcc tgacgccgcc gcaccacgac cggttagccg gtttctacaa cgatgatgat
    27361 aaggaggcgc tgtgacgcaa tctgcaatgt gcatcccgct gtggcccgcc cggaacggca
    27421 atactgcgca tctggtcatg tgccctttcg ctggcggcag cagtagcgcg tttcgccact
    27481 ggcaagctga gcaactggct gattgcgcgc tttctctggt gacctggccg gggcgcgatc
    27541 gccttcgcca tctggaaccg ctcagaagca ttacacaact ggcggcactg ctggcgaacg
    27601 agctggaagc atccgtatcg cctgacacgc cgcttttact cgccgggcac agcatggggg
    27661 cgcaggtggc gtttgaaacc tgccgacttc tggagcaacg ggggcttgcg ccacaaggac
    27721 tgattatttc cgggtgccat gccccgcatc tgcattctga acgccagctc agccatcgcg
    27781 atgatgccga ctttatcgct gagctgatag acattggcgg atgttctcct gaactgcggg
    27841 aaaaccagga attaatgtcg ctgtttcttc ctcttctgcg cgctgatttt tacgccaccg
    27901 agagctatca ctacgactcg cccgacgtct gtccgccgct gcgcacgcct gcgctgttat
    27961 tgtgcggcag ccacgatcgc gaagcctcct ggcagcaggt cgatgcctgg cgtcagtggc
    28021 tgagccacgt tacaggcccg gtggtgattg acggcgatca cttctatccc attcaacaag
    28081 cccggtcctt ttttacgcag attgtccgcc attttcccca cgcattttct gcaatgaccg
    28141 cgtggcaaaa acagcccagt acttcagaaa ggtgacgcat gaattcttcc tttgaatctc
    28201 tgattgaaca gtatccctta cccattgccg aacagttgcg ccactgggcg gcccgttatg
    28261 cctcgcgaat tgccgtcgtt gatgcaaagg ggtcgttaac ctacagcgcg cttgatgcac
    28321 aagttgacga acttgccgca ggtctgtcat cactgggttt gcgttcgggg gagcatgtaa
    28381 ttgtgcagct tcccaacgac aacgcgtttg ttaccctgct gttcgccttg ttaagactgg
    28441 gcgttatccc cgtgctggcg atgccctcgc aacgggcgct ggatatcgac gcgctgattg
    28501 agctggcgca acccgtcgct tacgttattc acggggaaaa ccacgcagag ctggcccgac
    28561 agatggcgca caaacacgcc tgcttgcgtc atgttctggt cgctggagag accgtgagcg
    28621 acgattttac gccgctcttc tcccttcacg gtgagcgaca ggcatggccg cagcctgatg
    28681 tttccgccac cgcgttgttg ttgctctcag gcggcacaac cggcacgccc aaactcatcc
    28741 cgcgccgaca tgccgactat agctataact tcaacgcttc tgctgaactg tgcggcatca
    28801 gccaacagag cgtgtatctc gccgtcctcc cggtggcgca taactttccg ctggcctgcc
    28861 ccggtattct gggaacgctt gcctgcggcg gaaaagtggt gctgaccgac agcgccagct
    28921 gtgatgaggt gatgccttta atcgcgcagg aaagagtgac tcacgtcgcc ctggttccgg
    28981 cgctggcgca attatgggtg caggccaggg agtgggaaga cagcgacctt tcgtcgctgc
    29041 gcgtcattca ggcaggcggc gcccggctcg acccgacgct tgctgagcag gttatcgcca
    29101 cctttgactg taccctgcaa caggttttcg gtatggcgga aggcctgctc tgttttaccc
    29161 ggctggacga tccgcatgcc accattctcc acagccaggg gcgcccgttg tcccctctgg
    29221 atgaaatccg catcgttgat caagacgaga acgacgtcgc gccgggcgaa accgggcaat
    29281 tgttaacgcg cggcccttat accatttcgg gctattaccg cgcccctgcc cacaacacgc
    29341 aggcctttac cgcgcaaggg ttttaccgca caggcgacaa tgtcaggctg gatgaggtgg
    29401 ggaacctgca cgttgaggga cgcataaaag agcagatcaa ccgcgccgga gaaaaaatag
    29461 ccgcggctga agtggaatcg gcactgctgc gtttagcgga agtgcaagat tgcgcggtgg
    29521 tcgccgcgcc ggacacgctg cttggcgagc ggatttgcgc gtttatcatc gcgcagcagg
    29581 tgccaactga ctatcagcag ttgcgtcaac aactgacccg tatggggctc agcgcgtgga
    29641 aaattcctga ccaaatcgag tttctggacc actggccgct caccgccgtc ggcaagatag
    29701 acaaaaaacg cctgacggct ctcgccgtcg accgttatcg ccattctgcc caataagcgc
    29761 aaaccgaccc gaaacaggtt gaaataaacc cgtttcgggt agcaccacta ttagaaatag
    29821 ttatcatttt caattcacca ttgtcggtat ttttggcgtt tcgccgtctt acagggactc
    29881 acaacaatga aaatgacacg gctttatcct ctggccttgg ggggattatt gctccccgcc
    29941 attgctaatg cccagacttc acagcaagac gaaagcacgc tggtggttac cgccagtaaa
    30001 caatcttccc gctcggcatc agccaacaac gtctcgtcta ctgttgtcag cgcgccggaa
    30061 ttaagcgacg ccggcgtcac cgccagcgat aaactcccca gagtcttgcc cgggctcaat
    30121 attgaaaata gcggcaacat gcttttttcg acgatctcgc tacgcggcgt ctcttcagcg
    30181 caggacttct ataaccccgc cgtcaccctg tatgtcgatg gcgtccctca gctttccacc
    30241 aacaccatcc aggcgcttac cgatgtgcaa agcgtggagt tgctgcgagg cccacaggga
    30301 acgttatatg gcaaaagcgc tcagggcggg atcatcaaca tcgtcaccca gcagccggac
    30361 agcacgccgc gcggctatat tgaaggcggc gtcagtagcc gcgacagtta tcgaagtaag
    30421 ttcaacctga gcggccccat tcaggatggc ctgctgtacg gcagcgtcac cctgttacgc
    30481 caggttgatg acggcgacat gattaacccc gcgacgggaa gcgatgactt aggcggcacc
    30541 cgcgccagca tagggaatgt gaaactgcgt ctggcgccgg acgatcagcc ctgggaaatg
    30601 ggctttgccg cctcacgcga atgtacccgc gccacccagg atgcctatgt gggatggaat
    30661 gatattaagg gccgtaagct gtcgatcagc gatggttcac cagacccgta catgcggcgc
    30721 tgcactgaca gccagaccct gagtgggaaa tacaccaccg atgactgggt tttcaacctg
    30781 atcagcgcct ggcagcagca gcattattcg cgcgccttcc cttccggttc gttaatcgtc
    30841 aatatgcctc agcgctggaa tcaggatgtg caggagctgc gcgccgcaac cctgggcgat
    30901 gcgcgtaccg ttgatatggt gtttgggctg taccggcaga acacccgcga gaagttaaat
    30961 tcagcctacg acatgccgag aatgccttat ttaagcagta ccggctatac caccgctgaa
    31021 acgctggccg catacagtga cctgacctgg catttaaccg atcgttttga tatcggcggc
    31081 ggcgtgcgct tctcgcatga taaatccagt acacaatatc acggcagcat gctcggcaac
    31141 ccgtttggcg accagggtaa gagcaatgac gatcaggtgc tcgggcagct atccgcaggc
    31201 tatatgctga ccgatgactg gagagtgtat acccgtgtag cccagggata taaaccttcc
    31261 gggtacaaca tcgtgcctac tgcgggtctt gatgccaaac cgttcgtcgc cgagaaatcc
    31321 atcaactatg aacttggcac ccgctacgaa accgctgacg tcacgctgca agccgcgacg
    31381 ttttataccc acaccaaaga catgcagctt tactctggcc cggtcgggat gcagacatta
    31441 agcaatgcgg gtaaagccga cgccaccggc gttgagcttg aagcgaagtg gcggtttgcg
    31501 ccaggctggt catgggatat caatggcaac gtgatccgtt ccgaattcac caatgacagt
    31561 gagttgtatc acggtaaccg ggtgccgttc gtaccacgtt atggcgcggg aagcagcgtg
    31621 aacggcgtga ttgatacgcg ctatggcgca ctgatgcccc gactggcggt taatctggtc
    31681 gggccgcatt atttcgatgg cgacaaccag ttgcggcaag gcacctatgc caccctggac
    31741 agcagcctgg gctggcaggc gactgaacgg atgaacattt ccgtctatgt cgataacctg
    31801 ttcgaccgtc gttaccgtac ctatggctac atgaacggca gcagcgccgt cgc
//
LOCUS       DARNVM010000041        31492 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.041, whole genome shotgun sequence.
ACCESSION   DARNVM010000041 DARNVM010000000
VERSION     DARNVM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.041"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            452..967
                     /locus_tag="SI621_001677"
     CDS             452..967
                     /locus_tag="SI621_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4064405.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLEQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1185..1913
                     /gene="artP"
                     /locus_tag="SI621_001678"
     CDS             1185..1913
                     /gene="artP"
                     /locus_tag="SI621_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HEI4064406.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1931..2662
                     /gene="artJ"
                     /locus_tag="SI621_001679"
     CDS             1931..2662
                     /gene="artJ"
                     /locus_tag="SI621_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4064407.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2669..3385
                     /gene="artQ"
                     /locus_tag="SI621_001680"
     CDS             2669..3385
                     /gene="artQ"
                     /locus_tag="SI621_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HEI4064408.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPG"
     gene            3385..4053
                     /gene="artM"
                     /locus_tag="SI621_001681"
     CDS             3385..4053
                     /gene="artM"
                     /locus_tag="SI621_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HEI4064409.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLAVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4279..5010
                     /gene="artJ"
                     /locus_tag="SI621_001682"
     CDS             4279..5010
                     /gene="artJ"
                     /locus_tag="SI621_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HEI4064410.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQEQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5039..6166)
                     /gene="rlmC"
                     /locus_tag="SI621_001683"
     CDS             complement(5039..6166)
                     /gene="rlmC"
                     /locus_tag="SI621_001683"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HEI4064411.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLAKQ"
     gene            complement(6207..6695)
                     /locus_tag="SI621_001684"
     CDS             complement(6207..6695)
                     /locus_tag="SI621_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HEI4064412.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILLLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6755..7600)
                     /gene="potI"
                     /locus_tag="SI621_001685"
     CDS             complement(6755..7600)
                     /gene="potI"
                     /locus_tag="SI621_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HEI4064413.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(7597..8541)
                     /gene="potH"
                     /locus_tag="SI621_001686"
     CDS             complement(7597..8541)
                     /gene="potH"
                     /locus_tag="SI621_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEI4064414.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8551..9684)
                     /gene="potG"
                     /locus_tag="SI621_001687"
     CDS             complement(8551..9684)
                     /gene="potG"
                     /locus_tag="SI621_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HEI4064415.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFECVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9779..10891)
                     /gene="potF"
                     /locus_tag="SI621_001688"
     CDS             complement(9779..10891)
                     /gene="potF"
                     /locus_tag="SI621_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HEI4064416.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11242..11718)
                     /locus_tag="SI621_001689"
     CDS             complement(11242..11718)
                     /locus_tag="SI621_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEI4064417.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11806..12708)
                     /gene="rimK"
                     /locus_tag="SI621_001690"
     CDS             complement(11806..12708)
                     /gene="rimK"
                     /locus_tag="SI621_001690"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEI4064418.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12769..13491)
                     /gene="nfsA"
                     /locus_tag="SI621_001691"
     CDS             complement(12769..13491)
                     /gene="nfsA"
                     /locus_tag="SI621_001691"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HEI4064419.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13475..13762)
                     /locus_tag="SI621_001692"
     CDS             complement(13475..13762)
                     /locus_tag="SI621_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HEI4064420.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPTAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13922..14179
                     /gene="grxA"
                     /locus_tag="SI621_001693"
     CDS             13922..14179
                     /gene="grxA"
                     /locus_tag="SI621_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HEI4064421.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14209..14586)
                     /locus_tag="SI621_001694"
     CDS             complement(14209..14586)
                     /locus_tag="SI621_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HEI4064422.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            14856..16541
                     /locus_tag="SI621_001695"
     CDS             14856..16541
                     /locus_tag="SI621_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HEI4064423.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16716..17252)
                     /gene="rcdA"
                     /locus_tag="SI621_001696"
     CDS             complement(16716..17252)
                     /gene="rcdA"
                     /locus_tag="SI621_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HEI4064424.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17336..18544
                     /locus_tag="SI621_001697"
     CDS             17336..18544
                     /locus_tag="SI621_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064425.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            18544..19359
                     /gene="ybjI"
                     /locus_tag="SI621_001698"
     CDS             18544..19359
                     /gene="ybjI"
                     /locus_tag="SI621_001698"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HEI4064426.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            19444..19728
                     /locus_tag="SI621_001699"
     CDS             19444..19728
                     /locus_tag="SI621_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064427.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(19769..21001)
                     /gene="mdfA"
                     /locus_tag="SI621_001700"
     CDS             complement(19769..21001)
                     /gene="mdfA"
                     /locus_tag="SI621_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HEI4064428.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLFVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            21286..21882
                     /gene="ybjG"
                     /locus_tag="SI621_001701"
     CDS             21286..21882
                     /gene="ybjG"
                     /locus_tag="SI621_001701"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEI4064429.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            21940..22698
                     /gene="deoR"
                     /locus_tag="SI621_001702"
     CDS             21940..22698
                     /gene="deoR"
                     /locus_tag="SI621_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HEI4064430.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(22745..23947)
                     /gene="dacC"
                     /locus_tag="SI621_001703"
     CDS             complement(22745..23947)
                     /gene="dacC"
                     /locus_tag="SI621_001703"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HEI4064431.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24194..24820
                     /gene="gstB"
                     /locus_tag="SI621_001704"
     CDS             24194..24820
                     /gene="gstB"
                     /locus_tag="SI621_001704"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HEI4064432.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEDRDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(24817..25932)
                     /gene="yliI"
                     /locus_tag="SI621_001705"
     CDS             complement(24817..25932)
                     /gene="yliI"
                     /locus_tag="SI621_001705"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HEI4064433.1"
                     /translation="MHRQFFFSVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKMTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26043..26426)
                     /gene="bssR"
                     /locus_tag="SI621_001706"
     CDS             complement(26043..26426)
                     /gene="bssR"
                     /locus_tag="SI621_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HEI4064434.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26639..27964
                     /gene="rimO"
                     /locus_tag="SI621_001707"
     CDS             26639..27964
                     /gene="rimO"
                     /locus_tag="SI621_001707"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEI4064435.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            28190..28576
                     /locus_tag="SI621_001708"
     CDS             28190..28576
                     /locus_tag="SI621_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4064436.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            28566..28895
                     /locus_tag="SI621_001709"
     CDS             28566..28895
                     /locus_tag="SI621_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEI4064437.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(28965..30293)
                     /locus_tag="SI621_001710"
     CDS             complement(28965..30293)
                     /locus_tag="SI621_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEI4064438.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQIVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGVSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSLYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(30301..>31492)
                     /locus_tag="SI621_001711"
     CDS             complement(30301..>31492)
                     /locus_tag="SI621_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEI4064439.1"
                     /translation="ESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEK
                     YAQCCDIVTFNADLYLLLCRENVQTFTRKIAMINDFDSSFGYRNLRIHKSAICEPLQG
                     ENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLV
                     YQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGE
                     FRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIE
                     RINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
                     KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGK
                     LVKE"
BASE COUNT         8001 a         8077 c         7639 g         7775 t
ORIGIN      
        1 actatcaaat ttcgctcatt ttcaaaaata caatgtaatt ttagcaatca tcttaatcgc
       61 aggatctctt attacatgtg aaaaattctg taatgattag acaataaccg cgttcattta
      121 tcacgcgtta taaatgtgaa actggcttct tgcagatgct cttaacctct acaggaggat
      181 ttatcccgcc atttcagaac tatcatattt accttaaatg atcatttcag ccatataaca
      241 cgctcaacct cacaaatggt ttacactgtc tattgtttgg gtaatttaag taagtcagct
      301 acgagcaacc ttgagtgggt agccagcaat ggactcaaac ctaatcaggt gttcagattg
      361 tttcagaatt gtactaaaaa caatgtatta aataatctga tagtgtttgg tcgtagtgtg
      421 acacagacag caagctaata aggaaatgat tatgcgctac cgttcactat ctctacttat
      481 tccatgtgca ctgctgctga gtgcctgtac caccgtcacg ccagcttata aagataacgg
      541 cccccgcacc ggttcctgtg tccagggtgg ccctgacagc gtggctcaac aattctatga
      601 ctaccgcatt caacatcgca gcaatgacat taccgccctg cgcccctatt tgagtgacaa
      661 actggcaacc ctacttagcg atgccagccg cgataatagt catcgtgaat tgttaagcag
      721 cgatccgttc tccagtcgaa ccaccttgcc agacagcgcc cacgttgcca gtgcatcgac
      781 tatccctaat cgtgatgccc gtaacattcc attgcgcgtg gatctcgaac agggtgacca
      841 gggatggcag gatgaagtgt taatgattca ggaaggccag tgttgggtca tcgacgatgt
      901 gcgctactta ggcggtagcg ttcacgccac agcgggtacg ctccgtcagt ccatcgagaa
      961 tcgctaaagc cttgatcaac caataaaccc cgtaaattgt ccggcatttc gtgaggaatg
     1021 ccgacattta tgctcgccga ccaccgcccc cgttattttg tgctatgttt attgaataat
     1081 gcgctttgct tttaactttt aaagcagaaa tattgcataa ttattctgtc aaaggtacta
     1141 tcttcgacct caatagctat cagactgcca gtatacgagt gtcaatgagt attcaattaa
     1201 acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt cgatatcacg ctggattgcc
     1261 cacagggcga aacgctggtg ttacttggcc ccagcggcgc gggtaaaagc tcgctgctgc
     1321 gtgtactcaa tctgcttgag atgccgcgct ccggtacgct caacattgca ggcaaccatt
     1381 tcgatttcac caaaacaccc tctgacaaag cgattcgcga tttgcgtcgt aacgttggca
     1441 tggtgtttca gcaatacaac ctgtggccgc atctgaccgt gcagcaaaac ctgattgaag
     1501 cgccctgccg tgtactgggg ttgagcaaag atcaggcgct ggcccgtgca gaaaaactgc
     1561 tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc gctgcatctt tctggtggtc
     1621 agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga accgcaggta ctgctgttcg
     1681 atgaaccgac cgccgcactg gacccggaaa ttacggcaca aatcgtcagc atcattcgtg
     1741 agttggcaga aacgaatatt acccaggtga tcgtcaccca cgaagttgaa gtggcgcgta
     1801 aaaccgccag ccgagtggtg tatatggaaa acggtcatat cgtagaacaa ggcgacgcga
     1861 gctgctttac cgagccgcaa accgaagcat ttaaaaacta tctctctcac taatattcgg
     1921 gataacaaca atgaaaaaag ttctgattgc cgcgttaatt gcaggtttta gtctttccgc
     1981 cacagctgcc gaaactattc gttttgctac cgaagcctcc tatcctccgt ttgaatcgat
     2041 tgatgcaaac aaccagatcg ttggttttga cgtcgacctg gcacaggcac tgtgtaaaga
     2101 gattgatgca acctgtactt tctctaacca ggcgtttgac agcctgatcc caagcctgaa
     2161 attccgtcgc gtagaagcag tgatggcggg catggatatc actccggagc gtgaaaagca
     2221 ggtgctgttt accacccctt actatgacaa ctctgccctg tttgtgggtc agcaaggcaa
     2281 atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc gttcagaacg ggacgacaca
     2341 ccagaagttc attatggata agcacccgga aatcaccacc gttccgtatg acagctacca
     2401 gaacgcaaaa ctggatctgc aaaatggtcg tatcgacagc gtatttggtg acaccgcagt
     2461 ggtaaccgaa tggctgaaag acaacccgaa actggcggca gtgggcgata aagtgaccga
     2521 taaagattat ttcggtactg gcctcggcat cgcggtacgt cagggcaaca ctgaactgca
     2581 gcagaaactc aacactgcgc tggaaaaagt gaagaaagat ggcacttacg aaaccatcta
     2641 caacaaatgg ttccagaagt aattcctgat gaatgaattt tttcctttag caagcgccgc
     2701 cgggatgacc gtcggccttg ccgtttgtgc attgattgtc gggctggcgc tggcgatgtt
     2761 ctttgcggta tgggagtctg caaaatggcg tcctgtcgcg tgggcaggtt cagcgctggt
     2821 aaccattctg cgtggcctgc cagaaattct ggtggtgctg tttatctatt ttggctcctc
     2881 gcagctgctg ctgacgcttt cggatggttt cactatcaat cttgggttcg tgcagatccc
     2941 ggtgcagatg gacattgaga acttcgacgt tagcccgttc ctttgtggtg tcatcgctct
     3001 gtcgctgctg tatgccgcct atgcctcgca aacgctgcgc ggcgcgttga aagcggtgcc
     3061 gatgggtcag tgggaatccg gccaggcgct ggggctgtcg aaatcggcta tctttttccg
     3121 tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggt ctcggtaacc agtggctggt
     3181 gctgctgaaa gataccgcgc tggtcagttt gattagtgtg aatgatttaa tgctacaaac
     3241 caaaagcatc gctacccgta cccaggaacc atttacctgg tacattgtgg cggcggcgat
     3301 ttacctggtg atcaccctgc tcagtcagta cattctcaaa cgcattgacc tgcgcgcgac
     3361 acgttttgag cggaggcctg gctaatgttt gagtatttac ccgaactgat gaaagggctg
     3421 cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg cactgattct ggcattgatt
     3481 tttaccatca tcctgacgct gaaaacgccg gtactggtgt ggctggtgcg gggttatatt
     3541 acgctgttta ccggtacgcc gctgctggtg cagattttcc tgatttatta cgggccgggc
     3601 cagttcccga cgttgcagga gtatccggca ctgtggcatt tgctgtcaga accgtggctg
     3661 tgtgcgctga ttgcgttgtc gctgaacagt gcagcgtata ccacgcagct gttttacggt
     3721 gccattcgtg cgatcccgga aggtcagtgg cagtcctgta gcgccctggg aatgagcaaa
     3781 aaagatacgc tggcgatcct gctgccgtat gcctttaaac gctcgctctc ttcttattcc
     3841 aacgaagtgg tgctggtgtt caaaagtacc tctctggctt acaccattac gctgatggaa
     3901 gtgatgggat acagccagtt gttgtacgga cgcacctacg atgtcatggt gttcggtgcg
     3961 gcagggatta tttacctggc cgtcaacggc ctgctgacgc tgatgatgcg tctgatcgag
     4021 cgcaaagcac tggcattcga acggcggaat taatgtggct gcgtgctgat gccggatgcg
     4081 gcgtgaacgc cttatccggc ctacatagtg cataaattct tataatgaag acggacaacc
     4141 cactaagttg tccgtctttt tttattccat gtaaattatt taatcatatt tattgcatat
     4201 aaattcactt gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg
     4261 acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact
     4321 ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt
     4381 gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg
     4441 tgcaaacaaa tgcaggcaga atgtactttt acgaatcacg cgttcgacag cctgatcccg
     4501 tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac cccggagcgt
     4561 agcaaacagg tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa
     4621 aaagatacct acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc
     4681 accacgcacc agaaatatat tcaggagcag cacccggaag tgaaaactgt ctcctatgac
     4741 agttatcaga atgcctttat cgacctgaaa aatggtcgta ttgatggggt atttggtgac
     4801 acagcggtgg taaacgaatg gctgaaaacc aatccacaac tgggcgttgc gactgagaaa
     4861 gtgaccgatc cgcagtactt cggcaccggc ctgggtatcg ctgtacgtcc ggataacaaa
     4921 gctctgctgg agaaactgaa taacgcgctg gcggcaatta aagctgacgg tacatatcag
     4981 aaaatcaatg accagtggtt cccacagtaa gattcagaaa gccgcacctg cggctttttt
     5041 attgcttcgc cagcagcgtc agcacttcat agtgcgcggt gtgcgggaac atatcaaaaa
     5101 gctgtacccg ttcaatacgg taacctggca gttcgcggat atctttcgcc atggtttggg
     5161 cgttacagct ggagtagata ataaaacgcg gtgccatcgt tgagagataa tcacacagcg
     5221 gtttaccaat gccacgtcgc ggcgggttaa ccagcaccag ctcaggcact tccccctggg
     5281 cggtggcaaa ctgagtggag tccagcgcct gaaattgcaa acgcgttaag cccagttcag
     5341 cggctgactg cttcgcacag gcaatggcct ctggtgcaat ttcgatcccg gttaactgca
     5401 tgtcaggcgt cgcgcagtgt aaaccaaagc cccccacgcc acagaacaga tcccacatat
     5461 gtttaactgg cagctgccgt acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg
     5521 ccggattagt ctggaagaaa ctttgcggac ggatccacag cggtacgtca ttaaaacgct
     5581 ctgccagcgc ctgttgttca gtcaggtaga tctccgtctc cccttccata atcgccatat
     5641 gtaccggctg aatattgacg gtaataactt tcagctgcgg tagttgttcc tgtaaccacg
     5701 gcagcgcctt acgcagttgc gccagcttgg tatcagaacg cagtacaaag cgcagcatca
     5761 tgccgccatc gctctggctt tcagtcagca gaatgtattt cagttcgcca cgcttacgcg
     5821 ccacgttgta gggcgttaac cccgctcggg cgataaacgg ttttagcgcc gcaaaaacgg
     5881 gcgcaaatga ggcaggataa agcgggcagt cacaaaggtc ttccggtgtg ccgtctcgat
     5941 gcagcatacc gagcagtggt ttttcaacgc taccactcac caccattttg gctttattac
     6001 gaaacccttg ttccgggcct gacactggcg cacaccattc ctcaaccgga aagtcggcga
     6061 gcagattttt aagatcggcg gttttagcgg agagttgctc tggaatcggc tgcgttatcc
     6121 actgacagga acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac
     6181 atcatcaggg gcgacgatta tacacattat tgcaactgga agaaccgccg actggtcgag
     6241 ggaacgaaca gtagcaacag gatgagcata tccggcaact tctgcagcat caggctatgg
     6301 aagatttcac gttttgattc accgggaatg ctgaacagct ccggataacc ataccccagc
     6361 gaagctgccc acaagtaact tgcggctgtg atttgtgtca acagatacag ccagcgcgcc
     6421 cagcgacgcc ctttaaccag cgaaaacgca caccagatct caatgaaaac cagcaccaga
     6481 ctgctcaaaa agaccagcgt taaactccag gtttgtacgc tgcgatgaat gaactcgcca
     6541 ataccgcgca cgcccagcgt attgaaaatc atcagcacgt cgaggccacg gatcataata
     6601 atggcgagcg ccgccacctg caccagcgca ggcacattca ggcgagcatg agatgaagat
     6661 gttttcttaa aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg acatgcgcgg
     6721 cattatgtag ccaggttggc aaattttagt gtcttcagcc acgtcttgca cgctggatgt
     6781 cgcgtatccg ctgtttttct gcgcgcgcca tcagatacca ggcgataaat ccgacaattc
     6841 cgaccgcgcc gaggatcaat gttgccaggg cgttgatttc cggattcacc cccatccgca
     6901 cgctggaaaa gaccagcatc ggtaacgtgg tggctcccgg cccggaaaca aagctggcga
     6961 tcaccagatc atcaagcgac aaagtaaaag ccagtaacca gccagaaatg atcgcgggca
     7021 tgatcatcgg tagcgtaatg acgaaaaaca ctttcaacgg cgtcgcaccg agatccatcg
     7081 ctgcttcttc tatcgagcga tccagttccc gcagacgcga cgaaataacg actgccacat
     7141 aagccgtaca gaaagtgaca tgcgccagcc agatggtgag cataccgcgg tccgcaggcc
     7201 agccaatggc atgagcaagc gcgacgaata acaacaatag cgacaagccc gtgatgacat
     7261 ctggcatcac cagcggcgcg gtgatcataa aggcaaaacc atttgatccg cgaaacctgc
     7321 caaagcgcac cagcacgacc gccgcaatcg tcccgaggat cgccgccgcc gtcgccgcac
     7381 aggccgcaat tgtcaggctt aaaccaaccg cactcatcat tgcgtcatcg cgcaataact
     7441 caccatacca gcgtgttgac cagccggccc acaccgtcac cagcttcgag ctgttgaatg
     7501 aatagatcac cagcatcagc attggcgcgt agagaaaggt gaagcccagc agcaaaatca
     7561 caatccgcca gggcgaacga actaccggta aattattcat ccgtgttctc ccacgctttt
     7621 ttgctgatgc ttgtggaacc acattatcgg cacaattagc agcagcaaca tgatgatcgc
     7681 taccgccgag gccaccggcc agtcgcggtt gttaaagaac tcctgccaca gcacgcgccc
     7741 gatcatgatg ctgtccgggc caccgagcag ttcagggatc acaaactcgc ccacagccgg
     7801 gataaacacc agcatcgatc cggcaataat cccgcctttg gtcagcggca cgatcacagt
     7861 aaagaacgtt ttcagcggtc gtgcaccgag atccagtgcc gcttccacca gcgaataatc
     7921 aatacgaatc aaggcggtgt agatcggcag aaccataaac ggcacgtagg cataaacaat
     7981 gccgatataa acggccagat tggtatgcag aatggtcagc ggctggtcga taacccccag
     8041 ccacagcaga aaattattca gcacgccgtt attttttaat attcccatcc aggcataaac
     8101 gcgaatcaga aacgaggtcc acgacggcag gatcaccagt agcaataaga tattacgggt
     8161 cgaaggctta ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta aacagcaaat
     8221 agtcgaaatc gccgccacct gtagcgactg gagataagca tcgaaataga gcggatcgtc
     8281 ggtcagttgc aggaaattac cgagattaag agtgatggaa agttgcccgt cagcccactc
     8341 catcagttcg gtataaggtg gaatggcgcg cgccatctcc gccaggctta ttttaaagac
     8401 aatcagaaat ggcagcagaa acagcaggat tagccagata tacggcagcg caatcaccag
     8461 tttgcgccca tgcttcattt gcaactgcga cagccacagc ttaaagggtt ttggctgggc
     8521 agcaggttca agtgtactca tctttgctcc ttaaaccgtc agcaccacac agctgtccac
     8581 ttcccagcac aaacgcactt catcgcccca ggtcggtaac cctttacgat gacgatgggc
     8641 gttttgtagc tgggcgctaa tcatctgccc acttttcaga cgaacgtgat acaccgaaag
     8701 atcgccgaga taggcaatgt gtatcacctc ccccaccgcg aagttacaac cattggcggg
     8761 cggctcttcg caaagcatga ttttttccgg acgcagcgct acgtgtaccg gcacgttatc
     8821 gaccaccgaa gcatctacat cgactttcag tggatgcacc agccccggcg aataaagcac
     8881 cagaccatct tcctggcgct ctttgagtac acattcaaag acattaaccg agccaataaa
     8941 ttcggcgctg tagcgggtgg tcggatgctc atagatctct tccggttcgc caatctggac
     9001 aaatttccca cgattcataa tggcgatgcg ccccgccatg gtcatcgcct cttcctgatc
     9061 gtgggtgacc atcacacaag tcacaccgac acgctccaga atatccacca cttcaagctg
     9121 catccggtca cgcagctttt tatccagcgc gcccataggc tcatcgagca gtaatagttt
     9181 cgggcgtttg gcaaggcttc gggccagggc cacacgctgt cgctgaccac cggaaagctg
     9241 atgcggtttg cgtttggcga actcctgcat atgcaccagg ccgagcatct cattgacccg
     9301 gctggcaatt tccgctttcg gcaatttgtc ctgtttcagg ccaaaagcga tattctgttc
     9361 cacggtcata tgcggaaaca gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta
     9421 aggcggaacc tgtgacaaat cgacgccatc gagcataatc tgcccggcag aaggttgttc
     9481 gaaacctgcc agcatacgca gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc
     9541 gaagatttcg cctttataga tggtcaggct gacatcatcg accgcatgct gaccatcgta
     9601 ggatttggtg aggttgcgga tttctaatag cggcgtcagc gccttacggg ttttcgcctg
     9661 cgggcgaggg atagcgtcat tcacggcagc tctccggcat tgaatgtgtg caagcgcacc
     9721 ataatggtgc gaattgccgg cgggtgtggt gcgcccgtcc ggcatctacg actgcggatt
     9781 attttccgct cttcactttg gtccacgcgc gggtgcgcac acggtcgatt ttcggatcct
     9841 gtactttcag agtgaacagc ttcgcacgaa catccgcagg cgggtaaata cccgggttat
     9901 cacggacttc cgcactcacc agcggcgtag ctgctttatt ggcgttagca tagaacacat
     9961 ggtcggaaat atgcgccacc acatccgggc gcagcaggta attcaggaac tgataggctt
    10021 cgtctttatt tttggcatcc gcaggcatgg cgaagacatc aaagaacgcc atcgcccctt
    10081 ctttcggaat cgagaacgag acattaacgc cattcttcgc ttccttcgcg cggtttgacg
    10141 cctgccagac atcgcccgcc cagccaatag cgacgcagat atcgccgttt gccaggtcgt
    10201 taatgtattg cgatgaatgg aaataacgaa tgttcgggcg cagctttaac agcagatcgg
    10261 ttgccggtcc ggtgtaatca tctgctttag tgctgttggg atctttaccg agataattca
    10321 gcacggtagc aaacacttct tccggtgcat caaggaaaga gacgccgcag cttttcagtt
    10381 tttccagatt ttcaggcttg aggatcaagt cccagctatc gacgggcgcg ttttcgccca
    10441 gcaccgcttt aactttatca acgttatagc caatcccggt cgtcgcccac atatagggca
    10501 tagcaaattt attatccggg tcgtgtttgg caaccaactt cagcagttcc gggtcgagat
    10561 tcttccactc cggcaattta cttttatcca gcggctggaa aactcccgca gtcaactggc
    10621 gttcaagaaa gcttgctgac ggcaccacca ggtcaaagcc ggtgctaccc gccattaatt
    10681 tgccttccag tacttcgtta gagtcgaaaa catcgtagac gactttaata ccggtttctt
    10741 tttcaaaatt ggccaccgtg tccggggcga tataatcaga ccagttataa atgtggagtg
    10801 ttttttgttc agccgcgagc gtgccgacag agacggccat cagagcaccc gcaaccagac
    10861 ccgatagcca ttttttattt aaggcggtca tatctctttc cttctgaaag ttcgttaaca
    10921 aacgaacaaa agttgtcaca ctgaaagata tgcaataaac gtgcatattt tttatcggcg
    10981 cggtgtaaga aaagagaact ctctcccggc cagcactgct cgctatataa aagcatcgca
    11041 agaataactt tagccagggt ttgagactat agctcggatt aaaaaacggt atttaacaaa
    11101 tttttatgcg tatttgctct atgtgataaa gccaaatcgg cacaagagaa gtgaaataat
    11161 ctttaagaaa aggttaaaaa cagcagataa ttttgcagca cgcagtcact gcggcagcga
    11221 ctgcgtactc gaaaggagtt atcagtggag gaaatagttt tcggtaacgt tgttttgccc
    11281 ttcatcgtca gtcggtaaca gcagttgatg ggcattagct tcaagaatga ccatcgaaat
    11341 ctgctcttcg ctctgacgca cgaaccatgc aaactgctca taggttacgc cctgcataac
    11401 ggataaagac tggcaaacca ccagctttgg cagattatca tcctgcatat caagaaatgc
    11461 tttcacggtc agcgaactgg cattgatggc agataaatct gccgccaggg gcaatacggc
    11521 tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg ttatcgatca gatcgatttt
    11581 ggcatcaaag acaccgtcga aattctgcat atggggcaga tgcagagcct gacagttatc
    11641 acattcaaaa aaactcatcc ccaggtcatc gagccattga cgcagcgtat ccagaccagg
    11701 aacgaccagc gatgtcataa ttttgtacaa cctctttcga taaaaagacc ggcacagctt
    11761 acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat tgcgactaac cacccgtttt
    11821 caggcaatat tctgtcgtag cgtggcgttc gatccagcgg atcattttac ccgcgatgtc
    11881 gataccggtg gttttttcta ttccttccag ccccggcgac gcattcactt ccatcaccaa
    11941 cggcccgcga ttagcacgca gaatatccac accagcaacg tccagcgcca tcgttcgcgc
    12001 ggcttttatc gcgatttcac gctcctgtgg tgtgatactg gcgacgcttg ccgcgccgcc
    12061 acgatgcaaa ttggaacgaa aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac
    12121 ttcatcgcca acaaccagac agcggatatc gcacccttgc gcctctttga tatattcctg
    12181 caccagaata tgcgcgttca gaccgcggaa agcgtcaatc acgctttcgg ccgcctgacg
    12241 cgtctccgcc agcacgacgc caattccctg cgtgccttca accaacttga ccaccagcgg
    12301 cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt
    12361 gacaggcagg tcgatgccct gacgcgccag cagttgcatg gaacgcaatt tgtcacgcgc
    12421 ccgggcaatg gcgaccgact cattgagcgg atagctcccc agcatctcga actggcgcag
    12481 tgccgccgtc ccataaaagg taatggcggt gccaatacgc gggatcactg cgtcaaaatg
    12541 gggtaacttg cggcctttgt agtgaataga agacgccgca ggatttatgt tcatgtagca
    12601 agaaagcgga tcaagaattt caaccaggtg accgcgctgt atagcggctt cacgcagccg
    12661 cttacacgaa tagagcgttc catcccggga caatatggca attttcaccc tgcacctctc
    12721 tgtcagacct ggtcaaaagc ccgcgtatca tacatcggcg tggcggtttt agcgcgtagc
    12781 ccaaccctgt ttgtgcaaat aatccagtat aaatgggcgg ctttctttaa tgattgttcg
    12841 gcggatatga tcgctccagg tatcccggcg attattgctg ccacgggtga ggtaatattc
    12901 cgccagttgt tcgtcatact gcgccagtgc gtctttatcc agcggttgat agttgttttc
    12961 atgcaccaaa atggaggccg gtaaacgcgg cttaagatcc ggattatccg caggccagcc
    13021 aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat ttcagcagtt ccgtcaccgc
    13081 ttcaatatta ttgcgcaggc cgccgatata taccccgccc aatcccagcg attccgctgc
    13141 ggttaacgca ttctgcgcca tcattgccgt atcaacgaca ccgagcagca attgttccgc
    13201 caggccgagc tgagcatccg gacagatctg taaatggcgg ttaaagtcgg cacagaacac
    13261 ccagaactcc gccgcttgcg ctacgtgttt ttgcccgccg gtcagcgtca ccagttcatc
    13321 acgtaacgct ttgtcggtaa tgcgaataat gctactgcac tgcaaaaaac tggaactgga
    13381 cgtcgcacgg gcgctgttaa taatcgcctc acgctgcgct tcggaaatgg gttcatcagt
    13441 gaaatggcga atggagcgat ggccacaaat aagttcaatg gttggcgtca ttatcttttt
    13501 ctctttctga acgtgaatat tgcggtggac ggttcatcag ctgtggggca agacgttttg
    13561 ccacctgaag aataaccacc accgctgtgg gaagcatgag caaaacaccg agaaaaatca
    13621 tcagaatctg cacttctggc cgagaaaatg gctcaggcag cgacagggag ccgcttaccg
    13681 acagcagcgc caccgccagc aacatcattc cgataaattc cagtatcaac acgcttttag
    13741 gcaatttacc gatcgcgcgc atacgcttcc ctctgcaaag tgagccttca gtctaaaact
    13801 tttcactgta ttgtgtttaa cagttatagc ttttagcaat taatgcaaca ggttaaacct
    13861 actttcagcg aatacatttt agcgtgatca ttacaggcat aaatctatga ggagagaaat
    13921 aatgcaaacc gttatttttg gtcgtccggg ttgcccttac tgtgtgcgtg caaaagatct
    13981 ggctgagaaa ttgagcaatg aacgcgatga ttttcagtat cagtatgttg atattcgtgc
    14041 ggaagggatc actaaagaag atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc
    14101 gcagattttt gtcgatcagc aacatatcgg cggctatacc gattttgctg catgggtgaa
    14161 agagaatctg gacgcctgat cgtctgacaa gccctcgcgt tgagggcttt attgattttt
    14221 tctgtgctgt ggtttaaaca aactactgat aaataagaaa cacagtgccc ccagcgcaca
    14281 ccagaacacc gcacttaata accatgccag ctcttgccag aatgagcgcg tcggtgaaaa
    14341 aaacagccgc ataataagca ttgaacaggg tgccgccagc attgcgccaa acagaggttt
    14401 caggacttct ctacgctgtg aaaagaagct ggcaactgct ccaggaagaa tgaaaaacag
    14461 caagccgatt tcaggatgcc cggcagcccg aaaagcgcct ttcatatgcg tcgccagaaa
    14521 aaggcacacc acaatgaaga ggacaaaaca gcagattgcc cccgcccaac gttgtttatg
    14581 tttcactcgt tcctcctgac actgcgtcta tcgaacacat ttttcgccag tgtggcgttc
    14641 agtaagataa agccgcttcg cattccatgc taatataggc caacgcaatt catatagccg
    14701 ttgataccta atgtgattac actagtaaaa tatattgtta ctttactatc gtttaggtgc
    14761 gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt accatttctt
    14821 tcaacagctt actagtaaac aagaagttag cctccgtgaa tataaacgtc gccgaattgt
    14881 taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta tgtctcggga
    14941 aattacgact tggttcgatc caactgggta attccattgg cgttttagtc gtatcgctgt
    15001 tattaggcca acaacatttc agcattaaca ccgacgcgct taatcttggc tttatgctgt
    15061 ttattttctg cgtcggggtc gaagccggac cgaacttttt ttccattttt tttcgcgatg
    15121 ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtg atcgccttag
    15181 ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta gcaggctcta
    15241 tgacgtcgac accggttctg gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa
    15301 gcaggcagct ctcactggca ctggataatc tgagcctcgg gtatgcctta acctatttaa
    15361 tcggtctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag catcaggact
    15421 tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacacagat gccaaccgta
    15481 aggtttattt gccggtgatc cgcgcctacc gcgtcggccc ggagctggta gcctggaccg
    15541 acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac cggctgctac attgaacgta
    15601 ttcggcgtaa cgggattctg gcaaatccag acggtgatgc cgtactacaa atgggcgatg
    15661 aaatagcgtt ggtaggctat cctgacgccc acgcccgact cgatcccagt ttccgtaatg
    15721 gcaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg
    15781 ttaaaaacca taacgctgtt ggcaaacgcc tggcacaact gaagttgacc gatcacggtt
    15841 gcttccttaa ccgcgtcatt cgtagccaga ttgagatgcc gattgatgac aacgtcgtgc
    15901 ttaacaaagg tgacgtttta caagtcagcg gcgatgcccg ccgggtaaaa accatcgccg
    15961 atcgcatcgg ctttatctcg attcacagcc aggtcactga cttgctggca ttctgcgcct
    16021 tctttgttat tgggctgatg atcgggatga tcaccttcca gttcagcaca ttcagtttcg
    16081 gcatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt atgcgtgcta
    16141 accacccgac cttcggttac attccgcagg gtgcattaag catggtgaag gagttcggct
    16201 tgatggtgtt tatggcaggc gttggtctga gcgctggtag cggtattaat aacggcctgg
    16261 gcgcgattgg cggtcagatg ttgattgccg gattgattgt cagtctggtg ccagtggtta
    16321 tctgcttctt gttcggtgct tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa
    16381 tgatgggcgc acgcacctgc gcgccggcaa tggagatcat cagtgataca gctcgcagta
    16441 atatcccggc gctgggctat gcgggcactt acgcaatcgc caacgtcctg ctgacgctgg
    16501 cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt tgccctgaaa
    16561 atgaaatttt tttgcacaac cgcagaactt ttccgcaggg catcagtctt aattagtgcc
    16621 actgcttttc tttgatgtcc ccattttgtg gagcccatca accccgccat tttggttcaa
    16681 ggttggtggg ttttttgttg cctaaaattt atctactacc ctgcaaccct ctcaaccatc
    16741 ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag cgtcatcccc
    16801 tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc gagcgtttgc
    16861 tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg gctagccagc
    16921 gcgtagagct ggtacatcag ctccatatta tccggcgttg caacctgtga gctgtagatc
    16981 atatcggtga tagcctggca tgcgcccgga gcatcactaa catcgctaaa aaatgcctga
    17041 tattgccggg acatgatctc agtaaaactg ctgaacgcct ccagcaacaa ctcatcaatt
    17101 cctgaaaaat agtaggtcat cgaccccaac ggtaccccgg caagggtagc aattttgcgg
    17161 tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg gataattttt
    17221 tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctctctgc aattgtgtac
    17281 aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt gatctatgac
    17341 cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta tgttcttctt
    17401 tttgccgggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc gcgatattct
    17461 ttctgtttcg atcgctgaaa tgggcggagt cctctttggt ctgtcgatcg gttcaatgag
    17521 cggtattctc tgctcggcgt ggttagtgaa acgctttggg acgcgtaatg tcatcttggt
    17581 cacgatgtcc tgcgcattga tcgggatgat gatactaagt ctggcactct ggctgacatc
    17641 gcccctgctc ttcgcggttg gtctcggcgt ctttggggca agttttggtt ctgcggaagt
    17701 ggcgataaac gttgaaggtg ctgccgttga gcgagaaatg aataagacgg ttttgccgat
    17761 gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga tggcactaac
    17821 ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag gcatcgcgcc
    17881 tatttatatc gccattcagg caatccctga tggtacgggc aaaaatgctg ccgatggcac
    17941 ccagcatggt gaaaaaggcg taccttttta tcgcgatatc cagttgctac tgattggtgt
    18001 tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac ccttattaat
    18061 ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg gttttaccct
    18121 ggggatgacc gttggacgct tcactggcgg ttggttcatc gaccgttaca gtcgcgttgc
    18181 cgtggttcgg gccagtgcac taatgggggc gttaggtatt gggctgatta tttttgtcga
    18241 tagcgcctgg gtcgctgggg tgtctgttgt actctgggga ctgggtgcct cgctgggctt
    18301 cccactgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc gcgtcagtgt
    18361 ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg gctatctcgg
    18421 cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc
    18481 tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga tggagaatag
    18541 ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag cgatcaaaaa
    18601 acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca aggcattcgc
    18661 tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc tgaaattgct
    18721 aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagcgaagg caaagatgtt
    18781 tttaatggcg agctgtcgaa ggatgcgttt gctactgtcg tggaacattt gctgacgcgc
    18841 ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct ataccctcaa aaaatatgac
    18901 gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt cgataacttt
    18961 gacaacttag aagatatctt ctttaagttt ggtctgaatc tttccgatga actgattcca
    19021 caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccggt ccacaccggc
    19081 aacggcagca tagatctgat tatccccggc gtacataaag ccaatggcct tcgccaactg
    19141 cagaaattat ggggaataga cgacagcgaa gtagtggtct ttggcgatgg cggtaacgat
    19201 attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg cagcgcggtc
    19261 gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact ggatgtgatc
    19321 gataaagtgc ttaaacacga agcgccattt aaccaataaa acggcagcac gcccggcaac
    19381 acaatgcctg aatgaagatt gcataattcg cggaaattaa cccgctgcat tatttcaggc
    19441 gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg
    19501 gcgatggcgc aaagtgtcac cgtcgatgtg ccgagtggat acaaagtggt tgtggttcct
    19561 gattcagtca gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg
    19621 gctcctgcgc cagctccggc ttaccgcccg catccttatg ttcgccatct tgccagcgta
    19681 ggtgaaggga tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc
    19741 tgatcgcaca aagcaatcag gcattttttt acccttcgtg cgaatttccc atctgtttat
    19801 cttttaaaaa gataaccatc agcaacagcc acaaaattcc gttgacaagg ttgaagagat
    19861 taaacagtcc gttgcccccg ttcagccagg catgtttgct gatttcaata ccgacggtaa
    19921 agatcagcat ttgcagcatc cccatcgccg cagaaaccgt acctttactc atatcgctgg
    19981 caaacagagt taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac
    20041 ttaacccggc ggtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga
    20101 caaacagacc aatcataatc ggccagccgc ccataataat cagcgaacgt acggtgcggc
    20161 gcgaggtcag acgcgctagc agcaagttac ccgcaattaa cgccccgaaa ataggcactt
    20221 gcagcaagcc atattcatag ctgctcaact gctcaccggt aatgatgata atcggcgact
    20281 gggcgatcca cgccagcaac ggcaggctaa cgaatcccag cgccagcgcc cccgccacaa
    20341 agcgaccgtt cttcagcacc agcttatagt cacgaccgag ttctttcagc gacagtttct
    20401 cgcctatacg cgtggcggtt tcaggcatgg ctcgttgcag accgaaaaag gagatcgctg
    20461 ccaatgcggc aaacaaaaca aacatcccct cccagggcag cacatggatc cacgccgcgc
    20521 ccaccagcgg accaagtagt ggagcaatca gcgccacgtt cgccattagc gcggtgatct
    20581 tgatacaaac cgcctcttcg aaggattcct gaattgcggc atatcccaca gcgccaatga
    20641 aacagaggct tatgccctgc aagaagcgca acagggtgaa ttgttcaatg ttttgcgcca
    20701 gtaatattgc cagacaggtg acgataaacc acaccactcc cgccagcatc accggacggc
    20761 ggccaatacg atccgacagt ggccccagga gccattgtaa aaacatcccg cccgccagat
    20821 acgcagtcat cgaagtagga acccaatcaa tgcccgcctg atattgttcc accacggcca
    20881 acataccggg ttgaatcata tcgttgccga tataggttga aaattcgtaa agcaccagac
    20941 agagagggaa aagtaacgcc tgacgtccaa gcctggcacc ggaagctaat ttattttgca
    21001 tgcaatttct tcgccaataa taatcgcgca aagtttaata aaagcgcagc taacgagaaa
    21061 gcgaattttg tagctgaaac cacggttaag cacattctta cattatcgcg agtatagcta
    21121 cgctttcttt aagttttatt taacctatgc ccgttacaat cacccaccgt aaacaggccg
    21181 cttgagggaa ataagacgat gccgctttac ccagtttaac ctgcacttta ttctcaacga
    21241 cttgcctgta ttggctccct tttaatcact ttgcgtcggg aagttatgct ggaaaatttg
    21301 aatctctctc tattctctct tattaacgcg acgccagact ctgcgccgtg gatgatctcg
    21361 ttggcgattt ttattgctaa agatttgatt accgtggtgc cgttgctggc cgcggtactt
    21421 tggctgtggg ggcttacagc acaacggcaa ctggtgataa aaatcgctat cgcgctggcg
    21481 gtcagcctgt ttgtgtcctg gacgatgggg catctttttc cgcacgaccg accctttgtc
    21541 gaaaatatcg gctataactt cctgcatcat gcggcggatg actcattccc gagcgatcac
    21601 ggtacggtga ttttcacctt tgcactggca tttttatgct ggcatcgcct gtggtccggc
    21661 tcacttttaa tggtgctggc cgtcgtcatt gcctggtcgc gcgtttatct tggcgtccac
    21721 tggccgctgg atatgctcgg tggattgctg gcaggtatga ttggctgcct tagcgcccag
    21781 attatctggc aagcgatggg gcataaactc tatcaacgtc tgcaatcgtg gtatcgcttc
    21841 tgttttgcat taccgatccg caaaggctgg gtgcgtgact gatctctgct aaaaagtgta
    21901 gtattgagcg gctcgcttca ataactattc agagggatta tggaaacacg tcgcgaagag
    21961 cgtatcgggc agctgctgca agaattaaaa cgcagcgata agttacatct taaagacgcc
    22021 gccgccctgc ttggggtttc ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg
    22081 cccgtcgttt tgctcggcgg ctatattgtt ctggaaccgc gcagcgccag ccattacctg
    22141 ttaagcgatc aaaaatcccg cctggtggaa gaaaaacgcc gggcggcaaa actggctgcg
    22201 acgctggtag aacccgatca gaccctcttt tttgactgtg gcaccaccac accgtggatt
    22261 attgaagcga ttgataatga aatccctttc accgccgttt gttattcgct aaataccttt
    22321 ctggcactga aagagaaacc ccattgccgc gcgtttctgt gtggtggtga atttcacgcc
    22381 agcaacgcca ttttcaaacc catcgatttt cagcaaacgc tgaataattt ttgcccggat
    22441 atcgcttttt attccgcggc gggggtgcat gtcagtaaag gcgctacctg ctttaatctt
    22501 gaagagttgc cggtaaaaca ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc
    22561 gaccacagta aatttggcaa ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat
    22621 attgttgtga gcgattgttg cccggaagat gagtatgtga agtacgcgca gacgcagcgt
    22681 attaagttga tgtattaatg acgtataacc ggatgacgtt tcgcgccatc cggttatcac
    22741 aaaattaaga gaaccagctg ccgaaccact gatggaattt catcatcacg aaatcccaca
    22801 tccgaccaaa gaatccgccc tcttccacat tttccatcac gatcagcgga cgctgctcaa
    22861 tggatttacc gttaagctgg aaatcaatgg tcccgacaac ctgacctttt ttcagcggtg
    22921 cggtaagctg cggttccgtt aacgtgtagc tcgctttcag gtttttcagc tgcccacgcg
    22981 gtatggtcac tgagcccgct tcgcctgccc cgagattcac ttcgctctta tctccaaacc
    23041 agacgcgctg agtcacaaag gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc
    23101 ggaaacccca ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc
    23161 ccagcactac ggagatcaaa cgcatatcgc cctgggtagc cgaagcaacc agattatatc
    23221 ccgcgcctgc cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca
    23281 gacggttacg gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg
    23341 cgtactcttc cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg
    23401 tactgaactg ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagac
    23461 ccagtttttt ggcataacca ttcatcagac caataaatga ctcctggctc ccggcaacgt
    23521 aatcagccag cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt
    23581 ctgccaccga aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg
    23641 gatttcccgt cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat
    23701 cggccttaag cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcggggt
    23761 ccagtttttc atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa
    23821 tccatgcacg cgcatccacg ctcggcgctt caacggtttg ttccgccgcg aatgccgttg
    23881 gggcaaaaag gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt
    23941 gcgtcataaa tgccacccga gtatccattc aaaaaaaata cgttatatcg ccgtatgata
    24001 agttcggtga ttaataacgc acacacaaac ttaaagaaac cgtaagttgg taaagttttt
    24061 aaagtttatg caatatcctg ccgcttcgct gcttctggtg tcattttttt gccagctgtt
    24121 gcttaaaagt taactttact tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg
    24181 aaaaggggtg agtatgatta cgctgtgggg tcggaataat tcaaccaacg taaaaaaagt
    24241 attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt
    24301 tgggataaat cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt
    24361 gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc
    24421 ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcaga
    24481 aaaatggatg gactgggcaa accagacgct cagtaatgct catcgcggga tcctgatggg
    24541 attagtcaga acaccaccgg aagatcgcga tcaggccgcc attgatgcca gttgtaaaga
    24601 gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctccgg
    24661 cgacgagttt ggtgtgggcg atatcgctat cgcaccgttt atctacaatt tgtttaacgt
    24721 tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg
    24781 ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag
    24841 taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac
    24901 atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc
    24961 gttgacgctc atcacaatga catctttatc tttcagcgcg ccaataaata atttttgctg
    25021 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacggcgg gcgaatcttt
    25081 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
    25141 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
    25201 cgggatatta atttcatcgc caccgcgcgg gccatgttca ttcagccata gtgcattact
    25261 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg
    25321 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
    25381 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
    25441 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
    25501 ggtagacagt tttggcatct ggcgaaagac ggtgcggaag tcggtcatct ttgagagatc
    25561 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
    25621 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
    25681 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
    25741 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattgtc
    25801 gggtaaaaag gccagcgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
    25861 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacag agaaaaagaa
    25921 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
    25981 aacaaaatca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
    26041 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
    26101 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
    26161 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
    26221 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
    26281 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
    26341 ttcctcagtt gcacgtatgc ggcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
    26401 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
    26461 taaatagtat ggctattttt tgagcaaaat gctgtccagg atcaattctt cacgccgaat
    26521 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
    26581 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
    26641 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
    26701 tgtcgattca gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag
    26761 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
    26821 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
    26881 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
    26941 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
    27001 acacaaccca ttcctgagcc tggtgccaga acaaggcgtg aaactgacgc cgcgtcatta
    27061 tgcctatctg aaaatttctg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
    27121 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
    27181 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
    27241 cgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
    27301 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
    27361 tccgcatgtg gacgacgtca tcccactgat ggcagagggc aaaatcctgc cctatctgga
    27421 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaaacgtc cgggttctgt
    27481 agatcgccaa ctggcgcgca tcaaacagtg gcgcaaaatc tgcccggaac tgaccctacg
    27541 ctcaaccttt attgttggct tccctggcga gacggaagaa gatttccaga tgctactcga
    27601 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
    27661 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
    27721 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
    27781 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
    27841 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg ttaagccggg
    27901 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
    27961 ttaattaatc ttaaggcaga tctaacaagg atattgttct tcgatctgcc tctgctaccc
    28021 ttcccgatgt cctaccctta cgcacaaaat cccctgacgc ataaatcgag aaaaatgaat
    28081 cgtactcgag gcaactctca ctttttcaca ccaaattaaa gttttgatcg caattacttg
    28141 aaataagcgt aatggttact atagtaaaca ttaccacaag gatggatgta tgggtgtgta
    28201 taaggcaaga cgattcagcc aaagcacgaa aaaacttggt attcacgata aagttctcat
    28261 ggctgccgct gaagaagtga tgcagggcat atgggaggct gatttaggta gcggtgtcat
    28321 caaaaaacgt ttacccctcc aacaaggaaa gagtggtgga gccagaacaa ttatcttttt
    28381 taaatcagca aatcatgtgt ttttttatga tggttggtcc aagagtggtc taagcagtaa
    28441 aggaaccaaa gaaatcgaag atgatgaact ggcggcctac aaaaaaatgg caaatgcatt
    28501 tctggccttt agcaataagc aaatagagga tttgatcgaa actggctttt tgattgaggt
    28561 gaaaaatgag cgataaatta caaatgatgc tggaagatgc aaaggaactg aatgcactgg
    28621 gcctgatgtc tgatagtgaa gttgatgcca ttgccgtcat ggtcaaagct cgtgaagtca
    28681 gcgcccgtat agccaaagtc aaatcaatga cgggtgaaga gatcaaagcc atccgcaccc
    28741 gatacggcat gtcacaatcc gtgctggcac atacaatggg aatgtcaaaa gaaagtgtct
    28801 cgaaatggga acgaaatgaa ataaaaccga gcggcccggc actacgtatt ttgaatacat
    28861 tggcaataaa agggccggaa gtttttgcaa agtaaaatgt ttgcaaatga tttaccacat
    28921 tcattagtac cccttgatta aggggtacta acactattct aattctactg ccagctaatc
    28981 tgtgccctgc cgtttttctt actcttatac agtcgctcat ccgccagcat tttcaagcgt
    29041 tcaacggtag gacattcatt tgaatgcgct acaccgatac tggtgcttaa attaatgctt
    29101 cccacctgga aatgaacaat ttcttttaga atcgatagca actcttccag cgttccaccc
    29161 gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ctttaccttt atctaccggg
    29221 atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg
    29281 aaagtgtcgt tgatgttttt gaatttatcg acgtcaaaca ggcacaaata tccgcctttt
    29341 tccggcaggg cttttaaatc ttcgtcaaag gattttctgc ccatcgcttt ggtcaggtca
    29401 tccaggaacg ccgagttata cagactgtag agttttttga catcggcagc tgaaaagaaa
    29461 agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt ctccacaata
    29521 ccacgattaa tcactctgcg taccagatag aggcaactga cacccgatat cactgccacc
    29581 aatgcggcaa agaaaataat caatgtacag acccgcaaat aaacatcttt tttatacgca
    29641 aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga aacggtgaag
    29701 atggcgtttt tagcattaaa ttcatcaccg gtcaccggtt taccgttctt aaacagagag
    29761 aacatcaggt tctcgctatc catcgccagc gattttgaca gtaaagaaaa acgctccaga
    29821 ccaagcaaaa atatcagact acgattatga gttaccggat agaggtaata gacgaaggtt
    29881 ttaccgtcaa aagagataaa ccctgtattt ttgacatgtc gatacttcac aatctgcata
    29941 atatctgcac aataattttt ctcgaactcc tttacctgaa tacattcgtc actggaaatc
    30001 agataaggac cgacatcgta caaataagag ggtgaaaccg agtcggcgta agctgtgcgc
    30061 aagccaacca acagctgttc ctccgtggga tttagcgtgt tgataagatc aattgccacg
    30121 ctacgccgat attcagcaat atttgccacc gcaaaagcag tatcaataac ggactgtttc
    30181 aggccataca cccgttcctt taccatttga gtgtagatcc cgcaggcaac tgctgaaatc
    30241 atgataaaaa tcagcagact gactgtaagt acgaacttat tgattctgga catacgtaaa
    30301 ctactctttt actaattttc cacttttatc ccaggcggag aacggcatgg gtttctggta
    30361 cagatacccc tgagcctgaa cacatcctaa tttgattaat tcgcgcgcaa cgtcttcgct
    30421 ctctacgcct tccgcaatca ccgggatccg gttatagcgt gccagatcga aaataatgcg
    30481 cacaattttt cccgcagtac cgaaaatatc gccggtaaat tgtgagtcca cctttatgct
    30541 atctggatta atttcataaa aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat
    30601 cgatatccgt accccttgtt tacgcagttg ttcaatattg agataaaaat gttcattaat
    30661 tcgctcgatc acggtttcag tgatctcaac acagaggctg ttcgccttca ggccattttc
    30721 tttcatgatg gtggtaaaac gctcatgaaa gtgtggttcg tttaactgcc agggcgaaac
    30781 attaatatgc agtaagaaat catccttcag cgacgcacgc tgactaaaat gtctgaattc
    30841 acccattgcc gttttaataa tctgataacc cagctcattg ataaacccga tatcttcagc
    30901 aatggtaatg aactttagcg gtgaaatgat cccccgctcc gcagacaccc aacggcacaa
    30961 cgcctccgcg cccagaatgg cgcgggtgtt aatatcaacg atcggttgat agacgaggaa
    31021 tagttcgcca atttccattg catggcgaat attacgcgca atccagatat tctcttctat
    31081 ttcgttgagt ttcgcgtcat cacagaaaat aaactctgag aacatatggt cacggatact
    31141 ggaaatcgcc agtttcagtt tttctgcgta actccaggcg ttttctccct gcaaaggttc
    31201 acaaatggca gacttatgga tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt
    31261 tatcatcgct attttacggg taaatgtctg tacattttcc cggcacagaa gtaaatagag
    31321 atcggcattg aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca
    31381 caatttcctg acgattttat tgcgttcatc aacaccaaac accgcttcca cggcttccag
    31441 accacacagt ttgatgatcc ccacagccag atactgatta ttgttggact ca
//
LOCUS       DARNVM010000042        30863 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.042, whole genome shotgun sequence.
ACCESSION   DARNVM010000042 DARNVM010000000
VERSION     DARNVM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.042"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            248..631
                     /gene="secE"
                     /locus_tag="SI621_001712"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="SI621_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEI4064440.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="SI621_001713"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="SI621_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEI4064441.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="SI621_001714"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="SI621_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEI4064442.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="SI621_001715"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="SI621_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEI4064443.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="SI621_001716"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="SI621_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEI4064444.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="SI621_001717"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="SI621_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEI4064445.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="SI621_001718"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="SI621_001718"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEI4064446.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="SI621_001719"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="SI621_001719"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEI4064447.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="SI621_001720"
     CDS             12676..13215
                     /locus_tag="SI621_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HEI4064448.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARIHLKRTILALPES
                     NVIHAGYGSYAVIEVDLNGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="SI621_001721"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="SI621_001721"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEI4064449.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIQEMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="SI621_001722"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="SI621_001722"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEI4064450.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAIADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="SI621_001723"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="SI621_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEI4064451.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="SI621_001724"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="SI621_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HEI4064452.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINTACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDTQDPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRATGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="SI621_001725"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="SI621_001725"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEI4064453.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSAQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAITLGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="SI621_001726"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="SI621_001726"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEI4064454.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     TRGDTEELTVRSSDYTKARLADDGLDELRFSGLLAPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19223..19699)
                     /locus_tag="SI621_001727"
     CDS             complement(19223..19699)
                     /locus_tag="SI621_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HEI4064455.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLVRAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19794..20567
                     /gene="nudC"
                     /locus_tag="SI621_001728"
     CDS             19794..20567
                     /gene="nudC"
                     /locus_tag="SI621_001728"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEI4064456.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20607..21671
                     /gene="hemE"
                     /locus_tag="SI621_001729"
     CDS             20607..21671
                     /gene="hemE"
                     /locus_tag="SI621_001729"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEI4064457.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21681..22352
                     /gene="nfi"
                     /locus_tag="SI621_001730"
     CDS             21681..22352
                     /gene="nfi"
                     /locus_tag="SI621_001730"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEI4064458.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22395..22985
                     /locus_tag="SI621_001731"
     CDS             22395..22985
                     /locus_tag="SI621_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HEI4064459.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23172..23444
                     /gene="hupA"
                     /locus_tag="SI621_001732"
     CDS             23172..23444
                     /gene="hupA"
                     /locus_tag="SI621_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HEI4064460.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23457..24152
                     /locus_tag="SI621_001733"
     CDS             23457..24152
                     /locus_tag="SI621_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HEI4064461.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24154..24579)
                     /gene="zraP"
                     /locus_tag="SI621_001734"
     CDS             complement(24154..24579)
                     /gene="zraP"
                     /locus_tag="SI621_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HEI4064462.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24817..26214
                     /gene="zraS"
                     /locus_tag="SI621_001735"
     CDS             24817..26214
                     /gene="zraS"
                     /locus_tag="SI621_001735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HEI4064463.1"
                     /translation="MRFMQRSKDTLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEA
                     DRQALLEKGNVLIRALESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGII
                     ILHSNPEMVGRALYSPDEMQQLKPEENSRWRLLEDAEKTPALEVYRLFQPMSLPWRHG
                     MHNMPRCNGKAVPQAEAQQAIFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLS
                     FFWYRRYLRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPA
                     GGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANS
                     REIQLRFTANDMLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKIS
                     VTDSGKGIAADQLEAIFTPYFTTKADGTGLGLAVVHNIVEQHGGTIQVASQEGEGATF
                     TLWLPVTITRKDPQG"
     gene            26211..27536
                     /gene="zraR"
                     /locus_tag="SI621_001736"
     CDS             26211..27536
                     /gene="zraR"
                     /locus_tag="SI621_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HEI4064464.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLADHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27533..28822)
                     /gene="purD"
                     /locus_tag="SI621_001737"
     CDS             complement(27533..28822)
                     /gene="purD"
                     /locus_tag="SI621_001737"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEI4064465.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     QEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLTDDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28834..30423)
                     /gene="purH"
                     /locus_tag="SI621_001738"
     CDS             complement(28834..30423)
                     /gene="purH"
                     /locus_tag="SI621_001738"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEI4064466.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7279 a         7981 c         8439 g         7164 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaatac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt agcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcaccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg ctctgctgaa
     1861 agaactggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaggg tgcaaacgct gaagctgcta aagctgcagg
     2041 tgctgaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagctgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtattga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgtgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgctatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagaa cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc tgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactccctgt ctgtatacgc gcagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctagtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgtgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggttgctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatta accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccagaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtaaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaattgctg
     6841 cgtgcgatct tcggtgagaa agcgtctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgctta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttctggtca gatccgcctg tacgacggcc gcactggtga acagttcgaa
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtact
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggtgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggtca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact tcgaatccta tgtggttatt gaaggcggta tgaccaacct
     8701 ggaacgccag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaaaatgg gtgcggaagc aatccaggcc ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcttgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgatatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctatgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtatacc ggctttgcat atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctataaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtactcat gaaggtatca tgatgactcc ggttatcgaa ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 aaactctgtc gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcattaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catcacgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattacg cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc ctggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggatct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc caaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgatggtcc
    11881 ggaagcgcca cacgacattc tgcgtctgcg tggtgttcat gctgttactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ctggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggggtag tttagggaaa gagcatttgt
    12541 cagaatattt aatgaatttc tggatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaagctcgg caagaattcg atactattcc tgtgtgactt
    12661 tcttaaggaa cgataatgaa acaggaagtg gagaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttc tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac taacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcatacactt aaaaagaacc
    12961 attttggcgc tgccagaaag caacgttatt catgctgggt atggtagcta tgccgtgatt
    13021 gaagtggatt taaacggagg agacaaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aacttcgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatgg cctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgtggtg
    13741 agaactgttc cagctcgggg tgattgtcgg catagccacc tggctgcgtt ttcgagaaag
    13801 cgctgacgtt attaattgcc agcggaatca cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagccggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacatgga cgcaggcgtg
    13981 ggaaggagac agagtagcgg ctttgccagt aatgctgttg cagccatagc aaatgttctg
    14041 caaccatata gcagtcaacg cgccagctgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgg cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gttttttgcc tttcagatgg tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agttgcttta actcggcgta ttccgtctcc gccagcggct
    14281 gtacttccat ctgtagtgaa gagaactgtt cacgcagggc agggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa aagcagatgt tcaaagccca
    14401 tctcctgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcggttgct catagagaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaagcta acagtgttgc caaatcgttg acgggtcaga cgctgcgccc
    14581 gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgctgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctactgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga tggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcgtcgacaa ccaccggaac
    15001 cgtggcttgc tggataataa tctccagcat ggcacgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttagccag tttgtgccta atgcttcacg tgccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tattcggtag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagcg tcaccagttg
    15421 gctgccggaa gcgcgaatag actccaccat cagttgtgat gaggcgaatt tcccggtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaaccgtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa cctgtagcgc ggcgcagcgc caggctgcgc
    15781 cactggctag acttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt
    15841 aacaacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctgga tcctgggtat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggtat taatctcctg acgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcta ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc
    16201 tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgaca acggcgatac tgccgacacc
    16561 cgtcgccatt accgcaggcg cgcgcgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagg cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atctccatat cgtcatgggt cgaaacgccc agtcgcagac ctgccgcgcg
    16801 gatggcgctg agatcggtgg cttgcaaatc ttcctgcccc agatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgtaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgcacgc ctgcatccaa cagacgttcg atccactgtg cgctgtccac
    17041 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga catatccgcc
    17161 attcccactt caatggtttg cgcggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttaccgga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcagta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgcgcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc ataacccggc
    17581 gcgatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgtacgt catattccca ggcgattttg gtcagttcac ccagcgtatg cagctcggca
    17761 aattgcgctt catcgttggc gtcctgaaca gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccagtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcgcgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac gtatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cgcggcgtcc tgctttggcg cgttttggcg ctaataagcc gctaaaacgt
    18601 agctcgtcga ggccatcatc tgccagtcgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgtatcgatc cacggctggc gtagttttgc cagtccttgc
    18721 tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcaaactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg ggttgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcctg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt tgaatgtaaa
    19201 ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg tcaagcacca
    19261 gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg atgtcagata
    19321 aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg ctggaatcgt
    19381 agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc gcggcgcgca
    19441 cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg aaatgtccgg
    19501 cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg tttagcctca
    19561 tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg agcagatgct
    19621 tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt tccgtcaggt
    19681 tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact gctcagtgag
    19741 aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga gctatggatc
    19801 gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa aaattatggt
    19861 tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg
    19921 cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag cagcggcgtc
    19981 acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt caactggccg
    20041 gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt tactgcgggc
    20101 atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc cgtgagcgtt
    20161 actatccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat tcgatcctcc
    20221 ttgcccagca tacccgccat cgtaacggtg tccatacggt acttgccgga ttcgtcgaag
    20281 tgggcgaaac cctcgagcag gcggtcgcgc gggaagtgat ggaagagagc ggaattaaag
    20341 ttaaaaactt gcgttatgtg acttcccagc cgtggccgtt tcctcagtct ttaatgaccg
    20401 cgtttatggc ggaatacgac agcggcgaga tcgttatcga cccgaaagaa ttgctcgagg
    20461 cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc
    20521 gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga tacactgacc
    20581 gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg
    20641 cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag gcgggtcgct
    20701 atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtca ctgtgcaaaa
    20761 acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg
    20821 cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg ctctattttg
    20881 aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac gtcgataaac
    20941 tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg cgtaccattc
    21001 gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg tggacgctgg
    21061 cgacctatat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt
    21121 atgccgatcc gcaggcgctg cacgcgctac tcgataaact ggcgaaaagc gtcactttgt
    21181 atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac acctggggcg
    21241 gcgtgcttac cgggcgcgat tatcaacagt tctcgcttta ttacatgcat aaaattgttg
    21301 atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt accaaaggcg
    21361 gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc ctcgactgga
    21421 caacggacat cgccgatgcg cgccgccgcg tgggcaataa agtcgcgttg cagggtaata
    21481 tggatccgtc gatgctgtac gcgccgccag cccgcattga agaagaagta gcgactatac
    21541 ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc attcatcagg
    21601 atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcttg tctgaacagt
    21661 atcaccgcta aggagcgatt atggatctcg cgtcattacg cgctcaacaa attgaactgg
    21721 cttcttctgt gatccgcgag gatcgactcg ataaagatcc gccggatctg atcgccggag
    21781 ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat
    21841 acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc atgccttaca
    21901 ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt
    21961 cgcaaaagcc ggatttagtg tttgtcgatg gtcacgggat ctcgcatcct cgccgtcttg
    22021 gcgtcgccag ccactttggt ttgctggtgg atgtgccgac cattggcgtg gcgaaaaaac
    22081 ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc ccgctgatgg
    22141 ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta
    22201 tcgctaccgg ccatcgggtc agcgtagaca gcgcgctggc gtgggtacaa cgctgcatga
    22261 aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg
    22321 cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat aatttccgta
    22381 tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc ctggaaagct
    22441 ggcagcacgt cacttttatg gcctgcttat gcgaacgcat gtaccctaat tacgccgtat
    22501 tctgtcagca aactggtttt ggtgatgggc agatttaccg ccgcattctc gatctcatct
    22561 gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg gagaaatttg
    22621 aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg gcaatcgatg
    22681 cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg
    22741 cagtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg actcaggctg
    22801 gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa gaatgggaca
    22861 ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc gagctgataa
    22921 aaggccttag ggcagacctg cgtgaggcgg gcgagagcaa tattggtata atttttcagc
    22981 aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc ttccctttcg
    23041 ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag
    23101 gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata aacacattgt
    23161 aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa gcagaactgt
    23221 ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact gagtctctga
    23281 aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac caccgcgctg
    23341 agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca gctaacgtac
    23401 cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc gtggcagtga
    23461 acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc
    23521 tggcaggcgc tctgtttctc actgcctgta gtcacaactc ttcacttcct ccctttaccg
    23581 ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac agcggcgata
    23641 atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacc acgcgagagt
    23701 atcgctggca gggcgataac cttacgctca tcaatatcaa tgtttacagc aaaccgccgg
    23761 tgaacattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa cgtgaatccg
    23821 atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat cgtgctgatc
    23881 agatccgcca gattagcgat gctttacgtc aggggagagt cgtgctgcgc caggggcgct
    23941 ggcatgcgat ggaacagacc gtgaccacct gcgaaggaca aaccattaaa cctgatttag
    24001 attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg
    24061 tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac tctgatttct
    24121 gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac ccatatgacc
    24181 gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa tacccgcttc
    24241 agccatcgca atatcccgtt tcacccgtaa ctcatctaac gactgacgca aattctccat
    24301 ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta acagggcatt
    24361 gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc
    24421 attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg
    24481 ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag
    24541 cgccatcatc accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt
    24601 cttactttgg gtattgcatc tttcgtgcca acaacgaaac gctgatatga cgggtaatct
    24661 ggcatgataa acgagtaaaa atgactcgcc tgttgtgggt agcgagtcat ttttactcat
    24721 tgaaacttaa gtctttgtgt tacagcgcag ggtaagcgct gctaaaagat ggcatgattt
    24781 ctgctgtcaa aaagggatga acaggcaaag aagaagatac gttttatgca acgttcaaaa
    24841 gacaccttag ccaaatggtt aagcgcgatc ctccccgtgg tcattgttgg gctggtggga
    24901 ttgtttgcgg taacggtgat tcgtgattat gggcgggcaa gtgaggctga ccgtcaggca
    24961 ttactggaaa aaggtaatgt gctcattcgc gctctggagt caggaagccg cgtagggatg
    25021 gggatgcgaa tgcaccatgt gcagcaacag gcgcttctgg aagagatggc aggccagcct
    25081 ggggtgttgt ggtttgcggt caccgatgcg cagggcatca tcattcttca cagtaacccc
    25141 gaaatggtcg ggcgtgcact ctattctcct gatgaaatgc agcagttaaa gccagaggaa
    25201 aactcccgct ggagactgct tgaggatgca gaaaaaacgc ctgcgcttga ggtctatcgt
    25261 ttgttccagc caatgtcatt gccttggcgg catggtatgc acaatatgcc gcgctgtaac
    25321 ggcaaagctg tgccacaggc agaagcacaa caggctattt tcatcgccgt tgacgccagc
    25381 gatctggttg caacccagag tggggaaaag cgcaataccc tgattatcct cttcgccctg
    25441 gcgacggtct tgctggcaag cgtgttgtca ttcttctggt atcgccggta tctgcgctcg
    25501 cgccagcttc tacaagatga aatgaagcgc aaagagaagc tggtggcact ggggcatctg
    25561 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg gctggcgaaa
    25621 tactttgccg aacgcgcgcc tgcaggggga gaagcgcatc aactggcgca agtgatggcg
    25681 aaagaggctg accgtttaaa ccgcgtggta agtgagttgc tggaactggt taagccaacg
    25741 catctggctt tgcaggcggt ggatctcaac actctgatta accactcatt acagctggta
    25801 agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga catgttgccg
    25861 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    25921 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg ccagcgaaag cggcgcaggc
    25981 gtgaaaatta gcgttactga tagcggtaag ggaattgcgg cggatcagct tgaagctatc
    26041 ttcaccccgt acttcaccac caaagccgac ggcaccgggt tggggctagc ggtcgtgcat
    26101 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggaggg agaaggcgca
    26161 acgttcaccc tctggcttcc ggtcactatt acgcgtaagg acccacaagg atgacgcacg
    26221 ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt
    26281 tgctgcgcgg ctggggctat aacgtcgcgt tggcgaacag cgggcgacag gcgctggagc
    26341 aggtgcggga acaggttttt gatcttgtgc tttgcgatgt gcgaatggca gagatggacg
    26401 gcatcgccac gctcaaagag atcaaagcgt taaatccggc aattccggta ctgattatga
    26461 ccgcgtactc cagcgtcgag acggcggtag aggcgttgaa aaccggggcg ctggattatc
    26521 tcatcaagcc gctggatttc gataacctgc aggcgacgct ggaaaaggcg ctcgcacata
    26581 cgcacagtgt tgacgctgaa acgcctgcgg tgtctgccag ccagttcggt atggtcggta
    26641 aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    26701 cggtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg
    26761 ccagtagcgc acggagtgaa aaaccactgg taacgctcaa ctgtgcggca ctcaacgaat
    26821 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttaccgga gccgacaaac
    26881 ggcgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    26941 atatctcgcc gatgatgcag gttcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    27001 gtgtcggcag caaccagacc atctcggttg atgtccggct gattgccgcg acccatcgcg
    27061 atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaacg
    27121 tggtggcgat tgaagtaccg tcgctgcgtc agcggcggga agatattcct ctgctggctg
    27181 accattttct tcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc
    27241 aggcgatgga tctgctgatt cactacgact ggccgggaaa tattcgtgaa ctggaaaacg
    27301 cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc
    27361 tggcgattgc gagtacgccg atcccgctgg tacaaagtca ggatattcag ccgctggtgg
    27421 aagtcgaaaa agaggtgatt cttgcggcac tggagaaaac gggcggcaac aaaaccgaag
    27481 ccgcccgtca gttagggatc acgcgcaaaa cgctgttggc aaaactgtcg cgttagttct
    27541 gctcgcgttc gatagcgcgc cagccgatat ctttccggca gaagcagtcg tcccaatgga
    27601 tatcggtcat taaggcataa gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    27661 tgacgcacag tacgcgcccg ccgctggtta ccacctgctc gtcatccgtc agttttgtgc
    27721 ccgcgtggaa cactttgccg cctgccactt cttccagcgg caggccgtgg atcacatcac
    27781 cggtgcgata atcacccgga tacccgcccg cagccattac cacgccgaga gaagcacgtt
    27841 catcccactc ggacgttttc tcgtccagct tgccttcaca ggccgccagg caaagctcaa
    27901 ccagatcgga cttcatgcgc agcataatcg gctgggtttc tggatcgcca aagcggcagt
    27961 taaattcgat aaccttcgga ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac
    28021 cggtgtaggt gttgccttct gccgccatgc ctttcacggt tggccagatg attcgttcca
    28081 tcgtgcgctg atgaacctca tcggtcacta ctggcgcagg ggagtaagcg cccatcccgc
    28141 cggtgtttgg cccggtatct ttatcgccta cgcgtttgtg atcctggctg gtggccatcg
    28201 gcagcacatg ctcaccgtcc accatcacga taaagctcgc ttcttcgccg tcgaggaact
    28261 cttcgataac gatgcgatgc cccgcgtcgc caaaagcgtt gcctgccagc atatcgtgaa
    28321 cagccgcttc cgcttcttcc tgcgtcatcg ccacgataac gcctttcccg gcagccagac
    28381 cgtcagcttt aatgacgatt ggcgcgcctt tctcacgcag atacgccagc gcaggttcta
    28441 cctcggtgaa gttctggtat tccgccgtag ggatctgatg gcgggccagg aaatccttgg
    28501 taaacgcttt cgagccttcc agctgcgcgg cacctgcggt tgggccgaag attttcagcc
    28561 cggcggcacg gaaggtatcc accacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    28621 ttagatcgat cttttcgttt tgcgcgaaat ccagcagcgc cgggatatcg gttacgccga
    28681 tagcgacgtt ttgcagtgtg ggttccagcg cagtgcctgc attacccgga gcaacaaaaa
    28741 cagtttcaac cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt
    28801 taccaatcac taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt
    28861 gaagagcatt gcaataccgt gttcatcggc ggcggcaatc acttcgtcat cacggataga
    28921 accgccaggc tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg
    28981 gaacgggaag aacgcgtcag aagccatcga ggaacctttg acttccagac cttcatcggc
    29041 cgctttaata ccggcgattt ttgcggagta cacgcggctc atctggcccg cgccaatgcc
    29101 gatggtcata ttgtttttgg catagacgat ggcgttggat ttcacgaact tcgccacctt
    29161 ccagcagaac agcgcatcac gcagctcttg ttcagtcggc tgacgtttgg tgacgacgcg
    29221 cagttcttcc gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac
    29281 gcgtttgaaa tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac
    29341 gttctgtttg gcggcggtga ttttcagggc ttcttcgctg gcggacggcg caataatcac
    29401 ttcaacaaac tgacgagaaa tgatggcctg cgcggtttcc gcatccagct cgcggttaaa
    29461 ggcgataatg ccgccgaatg cggaggtcgg gtcggttttg tacgcgcgat cgtaagcatc
    29521 aagaatggaa ttgccgatag ccacgccgca agggttggcg tgcttcacaa tcacacatgc
    29581 cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    29641 agagagggct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc
    29701 ttctatatag aaggcagctt gctggtggct gttctcgccg taacgcatat cctgcttctt
    29761 aatgaagttc aggttcagcg tgcgcgggaa gcgaccggcg gcttctttgc tttcaccgtg
    29821 gtaagccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg
    29881 ttcgaaggct ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttggc
    29941 atccatctct ttaataatgg cgtcgtagtc gctgctcttc accacgatgg cgacatcttt
    30001 atggttcttg gcggcggagc gcaccatcgt tgggccgccg atatcgatgt tctcaaccgc
    30061 atcttccagc gagcagcctt cacgggcaac ggtctgggcg aacggataca ggttaacaac
    30121 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    30181 gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    30241 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    30301 ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    30361 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    30421 catggtaaat cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    30481 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    30541 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtgtttac
    30601 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    30661 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg
    30721 cggcctgcgg agaactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    30781 acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg actctgaaag
    30841 aggaaagcgt aatatacgcc acc
//
LOCUS       DARNVM010000043        29739 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.043, whole genome shotgun sequence.
ACCESSION   DARNVM010000043 DARNVM010000000
VERSION     DARNVM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.043"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            415..1554
                     /gene="wza"
                     /locus_tag="SI621_001739"
     CDS             415..1554
                     /gene="wza"
                     /locus_tag="SI621_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HEI4064467.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1560..2003
                     /gene="wzb"
                     /locus_tag="SI621_001740"
     CDS             1560..2003
                     /gene="wzb"
                     /locus_tag="SI621_001740"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HEI4064468.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2006..4168
                     /gene="wzc"
                     /locus_tag="SI621_001741"
     CDS             2006..4168
                     /gene="wzc"
                     /locus_tag="SI621_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HEI4064469.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4297..5136
                     /gene="wcaA"
                     /locus_tag="SI621_001742"
     CDS             4297..5136
                     /gene="wcaA"
                     /locus_tag="SI621_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HEI4064470.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAITLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5139..5627
                     /gene="wcaB"
                     /locus_tag="SI621_001743"
     CDS             5139..5627
                     /gene="wcaB"
                     /locus_tag="SI621_001743"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HEI4064471.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5624..6841
                     /gene="wcaC"
                     /locus_tag="SI621_001744"
     CDS             5624..6841
                     /gene="wcaC"
                     /locus_tag="SI621_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HEI4064472.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKKGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAVFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6816..8033
                     /gene="wcaD"
                     /locus_tag="SI621_001745"
     CDS             6816..8033
                     /gene="wcaD"
                     /locus_tag="SI621_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HEI4064473.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8044..8790
                     /gene="wcaE"
                     /locus_tag="SI621_001746"
     CDS             8044..8790
                     /gene="wcaE"
                     /locus_tag="SI621_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HEI4064474.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTCEYLENLKGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8806..9354
                     /gene="wcaF"
                     /locus_tag="SI621_001747"
     CDS             8806..9354
                     /gene="wcaF"
                     /locus_tag="SI621_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HEI4064475.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9381..10502
                     /gene="gmd"
                     /locus_tag="SI621_001748"
     CDS             9381..10502
                     /gene="gmd"
                     /locus_tag="SI621_001748"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEI4064476.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIVVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10505..11470
                     /gene="fcl"
                     /locus_tag="SI621_001749"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10505..11470
                     /gene="fcl"
                     /locus_tag="SI621_001749"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEI4064477.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11473..11952
                     /gene="gmm"
                     /locus_tag="SI621_001750"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11473..11952
                     /gene="gmm"
                     /locus_tag="SI621_001750"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HEI4064478.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPEALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11949..13172
                     /gene="wcaI"
                     /locus_tag="SI621_001751"
     CDS             11949..13172
                     /gene="wcaI"
                     /locus_tag="SI621_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HEI4064479.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADVDV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGICQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13175..14611
                     /gene="cpsB"
                     /locus_tag="SI621_001752"
     CDS             13175..14611
                     /gene="cpsB"
                     /locus_tag="SI621_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HEI4064480.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGRLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14713..16083
                     /gene="cpsG"
                     /locus_tag="SI621_001753"
                     /gene_synonym="manB"
     CDS             14713..16083
                     /gene="cpsG"
                     /locus_tag="SI621_001753"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HEI4064481.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGTSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16138..17532
                     /gene="wcaJ"
                     /locus_tag="SI621_001754"
     CDS             16138..17532
                     /gene="wcaJ"
                     /locus_tag="SI621_001754"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEI4064482.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17534..19012
                     /gene="wzxC"
                     /locus_tag="SI621_001755"
     CDS             17534..19012
                     /gene="wzxC"
                     /locus_tag="SI621_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HEI4064483.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVMGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19084..20364
                     /gene="wcaK"
                     /locus_tag="SI621_001756"
     CDS             19084..20364
                     /gene="wcaK"
                     /locus_tag="SI621_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HEI4064484.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLAKYVFGRCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            20361..21581
                     /gene="wcaL"
                     /locus_tag="SI621_001757"
     CDS             20361..21581
                     /gene="wcaL"
                     /locus_tag="SI621_001757"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HEI4064485.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21592..22986
                     /gene="wcaM"
                     /locus_tag="SI621_001758"
     CDS             21592..22986
                     /gene="wcaM"
                     /locus_tag="SI621_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HEI4064486.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQIVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCENINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGRMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQISVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23161..24054
                     /gene="galF"
                     /locus_tag="SI621_001759"
     CDS             23161..24054
                     /gene="galF"
                     /locus_tag="SI621_001759"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HEI4064487.1"
                     /translation="MTNLKAVIPVAGLGMYMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24427..25512
                     /gene="rfbB"
                     /locus_tag="SI621_001760"
     CDS             24427..25512
                     /gene="rfbB"
                     /locus_tag="SI621_001760"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEI4064488.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGQAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            25512..26411
                     /gene="rfbD"
                     /locus_tag="SI621_001761"
     CDS             25512..26411
                     /gene="rfbD"
                     /locus_tag="SI621_001761"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEI4064489.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQETDATAPLNVYGETKLAGEKALQKHCAKHIIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAVDKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26470..27345
                     /gene="rfbA"
                     /locus_tag="SI621_001762"
     CDS             26470..27345
                     /gene="rfbA"
                     /locus_tag="SI621_001762"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEI4064490.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     IKNQYGQYLLKMISE"
     gene            27363..27773
                     /locus_tag="SI621_001763"
     CDS             27363..27773
                     /locus_tag="SI621_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HEI4064491.1"
                     /translation="MDIKLISLQKHGDERGALIALEEQRNIPFEVKRIYYILETLKGV
                     RRGFHAHKVTRQLAIVVKGACKFHLDNGKETKQVELNDPTIALLIEPYIWHEMYDFSD
                     DCVLLVIADDFYKESDYIRNYDDFIRRVNSIENS"
     gene            27760..28227
                     /locus_tag="SI621_001764"
     CDS             27760..28227
                     /locus_tag="SI621_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4064492.1"
                     /translation="MRIHKLSDVQTTSIGDGTTIWQFVVILKGAVIGNNCNICANTLI
                     ENNVVIGNNVTVKSGVYIWDGVKIEDNVFIGPCVAFTNDKYPRSKVYPDEFLHTIIRK
                     GASIGANATILPGIEIGEKAIVGAGSVVTKNVPPCAIVVGNPARFIKWVEDNE"
     gene            28220..29326
                     /locus_tag="SI621_001765"
     CDS             28220..29326
                     /locus_tag="SI621_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEI4064493.1"
                     /translation="MNKIDFLDLFAINQRQHKELVSAFSRVLDSGWYIMGEELEQFEK
                     EFAEYCGVKYCIGVANGLDALILVLRAWKELGYLEDGDEVLVPANTYIASILAITENK
                     LVPVLVEPDIETYNINPALIENYITEKTKAILPVHLYGLLCNMPEISAIARKYNLLIL
                     EDCAQAHGAIRDGRKAGAWGDAAGFSFYPGKNLGALGDAGAVTTNNAELSSTIKALRN
                     YGSHKKYENIYQGLNSRLDELQAALLRVKIHTLPEDTAIRQRIAEKYIREIKNPAITL
                     PVYEGQGAHVWHLFVVRIANREKFQSYLLEKGIQTLIHYPLPPHKQQAYQNMSSLSLP
                     ITEQIHDEVISLPISPVMSEDDVNYVIKVVNDYK"
     gene            29326..>29739
                     /gene="wzx"
                     /locus_tag="SI621_001766"
     CDS             29326..>29739
                     /gene="wzx"
                     /locus_tag="SI621_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O2/O50 family O-antigen flippase"
                     /protein_id="HEI4064494.1"
                     /translation="MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQV
                     QSLITIVAGTTSAPVSTGLVRYTAENWQEGQEACAPWWRACLRVTLFLFLLIIPVVIL
                     LSKNISELLFSDGQYTWLIIFACCILPFSILNTLIA"
BASE COUNT         7589 a         7041 c         7868 g         7241 t
ORIGIN      
        1 tataattctt aaaaatagcc aattaccgaa ttgttacctt gcctgctatt ccgttagctg
       61 taacacttcc tcctgcatta ttggagagcc aatattcaat taccataatg tcaccttata
      121 ttcggaagtt aatttccgta ataacccttt ctcatcaacg actgcatgtt gagagggttt
      181 taaaaattaa actaaatcag tgtattggta gctaaaaagc caggggcggt agcgtgtctg
      241 gatgcctgaa agaccagtct gaattattct gtcaatagcc tgcggatgaa atcaattttt
      301 ttaggactga tgccagttaa atttttattc aattaactgc aaggtaaatg tgcatggcga
      361 cattgctatt aatagtgcac aggataatta ctctgccaaa gtgataaata aacaatgatg
      421 aaatccaaaa tgaaattgat gccattattg gtgtcagtaa ccttgataag cggttgcaca
      481 gtacttccgg gcagcaatat gtcgacgatg ggtaaagacg tcatcaaaca gcaggacgct
      541 gatttcgatc tcgacaaaat ggtgaatgtt tatccgttga ccccgcgcct gattgatcaa
      601 ttacgcccac gacccaacgt agcgcgcccc aatatgacgc tggaaagtga gatcgcgaat
      661 taccagtatc gcgtcgggcc gggggacgtt cttaatgtca ccgtctggga tcacccggaa
      721 ctcaccacgc cagccggtca gtaccgcagc tccagcgaca ccggcaactg ggtacagcct
      781 gacggcacta tgttttatcc gtatatcggc aaagttcacg tggtggggaa aacgctcgct
      841 gaaatccgca gtgatattac cgggcgctta gcgacgtaca tcgctgaccc gcaggtggac
      901 gttaatatcg ccgccttccg ctcgcaaaaa gcctatatct ccggccaggt gaataaatcc
      961 ggccaacagg cgatcaccaa cgtgccactg accattctcg acgccatcaa cgccgcaggt
     1021 ggcctgaccg acaccgctga ctggcgcaac gtggtgttaa cacacaatgg tcgtgaagaa
     1081 cgcatttcat tgcaggcgct gatgcaaaac ggcgacctca atcagaatcg actgctttat
     1141 cctggcgata ttctctacgt gccgcgtaat gatgatctga aagtgtttgt gatgggtgaa
     1201 gtgaagaaac agagcaccct gaaaatggac tttagcggca tgaccctgac tgaagccctt
     1261 ggtaatgctg aaggcatcga catgaccacc tccaacgcca gcggcatctt tgtcattcgt
     1321 ccgctgaaag gcgagggtgg gcgtaacggc aagattgcca atatctacca gctggatatg
     1381 tccgatgcca cgtcgctggt gatggcgaca gaattccgcc tgcaacctta tgacgtggtg
     1441 tatgtcacca ccgcaccggt ttcccgctgg aaccgtctga tcaatcagtt gctgccaact
     1501 attagcggtg tccgttacat gacggataca gccagcgaca ttcataactg gtaatcgtca
     1561 tgtttaacaa cattttagtt gtctgtgtcg gcaatatttg ccgttccccg acggcggaac
     1621 gcttactgca acgttatcac ccggagctga aagtggagtc cgctggactc ggcgcgctgg
     1681 tcggtaaggg cgctgatcct accgctatca gcgtcgccgc agaacatcaa ctgtctctgg
     1741 aaggtcactg tgcacggcaa atcagccgtc gcctgtgtcg caactacgac ctgattttga
     1801 ccatggaaaa gcgccatatc gaacgcttat gcgagatggc acctgagatg cgcggcaaag
     1861 tgatgctgtt tggtcactgg gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc
     1921 gggaaacgtt tgcagcggtg tacacattac ttgaacggtc tgcccgccag tgggcgcagg
     1981 cattgaacgc agagcaggta taagaatgac agaaaaagta aaacaacatg ccgctccggt
     2041 aacgggcagt gatgaaatcg atattggtcg cctggttggc accgtcattg aggcgcgctg
     2101 gtgggtaatt ggcatcactg ccgtattcgc cctctgtgcc gtggtttaca ccttcttcgc
     2161 tacgccgata tacagcgccg acgcactggt acaaatcgaa caaagcagcg gcaattcgtt
     2221 agtgcaggat atcggatcgg cattagccaa caaaccgcct gcatcggacg ccgagatcca
     2281 gttgattcgt tcgcgcctgg tgcttggtaa aacggtggac gatctcgacc tcgatattgc
     2341 ggtgagcaaa aacacgttcc cgattttcgg tgcgggctgg gatcgcctga tgggacgcca
     2401 gaacgagacg gtaaaagtga ccacctttaa ccgtcctaag gagatggcgg atcaggtgtt
     2461 tacgcttaat gtgcttgatg acaaaaacta cactttgagc agcgatggcg gctttagcgc
     2521 ccgtgggcaa gcgggccaga tgctgaaaaa agaaggcgtc acgctgatgg ttgaagctat
     2581 tcacgccagc ccggggagtg agtttaccgt caccaaatac tccacgctgg ggatgatcaa
     2641 ccaactgcaa aacagcctga cggtaacgga gaacggcaaa gacgcaggcg tactgagcct
     2701 gacttatacc ggtgaagatc gtgaacagat ccgcgacatt cttaacagca tcgctcgtaa
     2761 ctatcaggaa caaaatattg agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct
     2821 cgcgcaacag ttaccggaag tacgtagccg cctcgatgtt gccgaaaaca aactgaatgc
     2881 cttccgtcag gataaagatt ctgttgatct gccgctggaa gcgaaagcgg tgctcgattc
     2941 gatggtgaac atcgacgcgc agttgaacga actgaccttt aaagaagcgg aaatctccaa
     3001 gctgtacacc aaagttcacc cggcgtaccg cacgctgctg gagaaacggc aggcgctgga
     3061 agacgaaaaa gccaaactta atggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat
     3121 tgtccgtctg acccgcgatg tcgagtctgg gcagcaggtc tatatgcaac tgctgaataa
     3181 agaacaggag ctgaaaatca ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc
     3241 ggcaatcacc cagcctggtg tgctgaaacc gaagaaaggg ctgattatcc tcggcgcaat
     3301 tatcctcggc ctgatgctct ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg
     3361 catcgaaagc ccgcaagtgc tggaagaaca cggtatcagc gtatatgcca gcatcccgct
     3421 gtcggagtgg caaaaagcgc gcgatagcgt caaaaccatc aaaggggtta aacgctataa
     3481 acagagccag ctactggcgg tggggaatcc aaccgatctg gcgattgaag ccatccgcag
     3541 tctgcgtacc agtctgcact tcgcgatgat gcaggcgcag aacaatgtgt tgatgatgac
     3601 cggggttagc ccgtcaatcg gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag
     3661 ccagaccaat aaacgggtgt tgttgatcga ctgcgatatg cgcaaaggct acacccacga
     3721 gctgttgggc actaataacg ttaatggcct gtcggaaatt ctgattggtc agggcgatat
     3781 tactaccgct gctaaaccga cctctattgc caaatttgac ctgatcccgc gcggtcaggt
     3841 gccgccaaat ccttctgaac tgttgatgag cgaacgcttt gccgaactgg tgaactgggc
     3901 gagtaaaaac tatgacctgg tgttgattga tacgccgccg attctggcag tgaccgatgc
     3961 ggcaattgtt ggtcgtcatg tcggaaccac gttaatggtg gcgcgttatg cggtcaacac
     4021 attgaaagaa gtggaaacca gtctgagccg ctttgagcaa aatggtattc cggtgaaagg
     4081 ggtgattctc aactccatct tccgccgcgc cagcgcgtat caggattatg gctattacga
     4141 atacgaatat aagtcggacg cgaaataacg agatccgctg tcggatgcga tgctgacgca
     4201 tcttatccga cctccgttca cgccgcatcc ggcaacagaa cccgtaaaca agcttataaa
     4261 ccggcagaac atctcgacac tccgggagaa caacctatga aagacaatcc gctgatttca
     4321 atctatatgc cgacctggaa ccgccaacaa ctggcgattc gggcgataaa atcggtcctg
     4381 cgccaggact acagtaactg ggagatgatc atcgtggatg attgctccac ctcctgggaa
     4441 cagctgcaac agtacgtcac cgccctcaac gacccgcgta tcacctacat tcacaacgat
     4501 attaacagcg gggcgtgcgc ggtacgtaac caggcgatta cgctggcgca aggggaatac
     4561 atcaccggga tcgatgatga tgatgaatgg acacccaacc gtctgagcgt cttcctcgcc
     4621 cataaacagc aactggtcac tcacgccttt ttgtacgcta acgactacgt ttgccagggc
     4681 gaagtctatt cccaaccggc aagtttgcca ctgtatccca aatcgccgta ttcacgccgc
     4741 ctgttctata aacggaatat cattggcaat caggtcttta cctgggcatg gcgtttcaaa
     4801 gagtgtttgt ttgataccga actgaaagcg gcacaggatt acgacatctt cctgcggatg
     4861 gtggtggagt atggcgaacc gtggaaagtg gaagaggcga cgcagatcct gcatatcaat
     4921 cacggtgaaa tgcagatcac ctcgtcaccg aaaaaattct ccgggtactt ccacttttac
     4981 cgcaagcaca aagacaaatt cgaccgtgcc agcaaaaaat atcagctgtt taccctctac
     5041 cagatccgca ataaacgcat gacctggcgc actttgctga cgctgctgtc ggtgcgtaac
     5101 ggcaaacgtc tggctgatgg tattcggggg cgctaaagat gctggaagat ctgcgcgcca
     5161 acagctggag tttacgcccg tgctgcatgg ttcttgccta tcgcgccgct catttttgtt
     5221 cggtgtggcg caaaaagaac gtcctcaaca atctgtgggc ggccccgctg ctggtgctgt
     5281 atcgcattat caccgaatgc tttttcggtt atgaaatcca ggctgccgcg accattggcc
     5341 gccgctttac tatccatcac ggttacgccg tagtgatcaa taaaaacgtg gtagcggggg
     5401 atgattttac cattcgccac ggcgtcacta tcggcaatcg tggtgccgat aacatggcat
     5461 gtccacacat tggcaacggc gtcgagctcg gtgccaacgt cattattctt ggtgatatca
     5521 cgcttggtaa caacgtcacc gtgggcgcgg gcagcgtggt gctcgactct gtcccggaca
     5581 acgcgctggt ggtgggagaa aaagcgcgag tgaaggtaat taaatgaata ttctgcaatt
     5641 taatgtgcga ctggcggaag gtggggcagc aggtgtggcg ttagatctcc accagcgcgc
     5701 gctgcaacag gggctggcgt cacattttgt ctacggttac ggcaaaggcg gcaaagagag
     5761 cgtcagccat cagaactatc cgcaggtcat caaacatacg ccgcggatga ccgcgatggc
     5821 gaacattgcc ctgtttcgtc tgtttaatcg cgatctgttc ggcaatttca atgagttata
     5881 tcgcaccatt actcgcacac cgggtccggt ggtcctgcat tttcatgtgc tgcacagcta
     5941 ctggttgaat cttaagagcg tggtgcgctt ttgcgaaaaa gtgaaaaacc acaaaccgga
     6001 tatcactttg gtctggacgt tacacgatca ctggagcgtt accggacgct gcgcctttac
     6061 cgacggttgt gaaggctgga aaaagggctg ccagaaatgc ccgaccttaa ataattatcc
     6121 gccggtgaag attgatcgcg cgcaccaact ggtggcgggc aaacgccagt tattccgcga
     6181 gatgctggcg ctgggctgtc agtttatttc ccccagccag catgtggctg atgctttcaa
     6241 tagtctgtac ggtccagggc gttgccggat tatcaataac ggtattgata tggcaaccga
     6301 agcgattctg gcggacttgc ctccggtgcg cgaaacccag ggcaagccga aaatcgcggt
     6361 ggtggcgcat gacctgcgtt acgacggcaa aactaaccag caactggtac gcgagatgat
     6421 ggcgctgggc gacaaaatcg aactgcatac ctttggtaag ttctcgccgt tcaccgctgg
     6481 caacgtggtt aatcacggct ttgaaactga caagcgcaag ttgatgagcg cgctcaatca
     6541 gatggatgcg ctggtattca gttctcgcgt cgataactac ccgctaattt tgtgtgaggc
     6601 gctatcgatt ggcgtgccgg tgattgccac ccatagcgac gcggcgcggg aagtgctgca
     6661 aaaatccggc ggtaaaaccg tcagtgaaga agacgtgctg caactggtcc agttaagcaa
     6721 accggaaatc gcgcaggcgg tatttggtac cacgctggct gagttcagcc agcgcagccg
     6781 tgccgcctac agtggacaac agatgctgga ggagtatgtc aacttctatc agaatctgta
     6841 gctacctgct gctgccgctg atctacctgc tggttaacgt caaaatcgcc cagcttggtg
     6901 aaagtttccc catcaccatc gtcacttttt tacctgtctt gttactgctg tttttagagc
     6961 gcatcagcgt taaaaaatta atgattgccc tggggattgg cgcgggactc acggcgttta
     7021 actacctgtt tggtcagtcg ttggatgcca gtaaatacgt cacttcaacc atgctgtttg
     7081 tctatattgt gatcattatt ggcatggtgt ggagtattcg ctttaaaacg atttcgcccc
     7141 acaaccaccg caagatatta cgcttttttt atctggtggt cgggttggtg gtggcgctgg
     7201 cggcggtgga gatggcgcaa attatcctca ccggcggcag cagtattatg gaatcgattt
     7261 cgaaatatct gatttacagc aacagctatg tgctgaattt catcaaattc ggcggcaagc
     7321 gcacgacagc actttatttc gaaccggcat ttttcgctct ggcattaatc tcaatttggc
     7381 tcagcatcaa acagtttggt atcaaaacgc ctaaaaccga tgctatgatt ctcgcaggga
     7441 taatattatc cggatcgttt tcaggggtta tgacctttat cctgttttat ttgctggagt
     7501 gggcatttca atatctgaat aaagaggcga ttaagaaaaa gttaccgtta gcattgattt
     7561 ctctggctgt attcctggtt ggtgtggtaa ttgcatttcc ttatatttcc acccgtctgg
     7621 gtgatttagg cacggaagga tcgtcatcgt attatcgtat tgtcggtccg ctggtgatgg
     7681 tcggttattc tttgacccat attgacggtg tagtcagatt tggctcactt tatgaatatg
     7741 tcgcatcatt cggaatattt aacggtgcgg atgtcggaaa aaccatagac aatggtttgt
     7801 atctgctgat tatttatttt tcctggttcg cagtgttttt atcgctgtgg tacatgggga
     7861 aagtgataaa aatgatgatc aacgcttttg gtgataaccg caattttcgc gtgcaattat
     7921 atctttttac tccggtatcg ctgtttttta ccggttcgat atttagcccg gaatatgcat
     7981 ttttaatcgt ctgtccgttt attttgcgaa aagcgttaaa tattacgagg taagaataag
     8041 aacatgttgc ttagcataat cactgtcgcg tttcgtaacc tcgcagggat agtcaaaaca
     8101 cacgcctcgc tggcgcatct ggcgcaggcg gacgatatca gcttcgaatg gattgttgtc
     8161 gatggcggtt ccaatgacgg cacctgtgag tacctggaaa atctcaaggg tatctataac
     8221 ctacgctttg tcagcgagcc agataacggt atctacgacg ccatgaacaa aggcattgcg
     8281 atggcacaag gcaagttcgc gttgtttctc aactcaggtg atatttttca tcaggatgcc
     8341 gcaaattttg tccgtaagtt aaaagtacaa aaagataacg tgatgatcac tggcgatgcg
     8401 ctgctggatt ttggcgacgg gcataaaatt aaacgtagcg ccaaaccggg ctggtatatt
     8461 tatcacagcc tgcccgccag tcatcaggcg atatttttcc cggtatccgg cttgaaaaaa
     8521 tggcgttatg acctggaata taaagtttcg tccgactacg cgctggcggc caaaatgtat
     8581 aaagccggtt atgcatttaa aaaactcaat ggtctggtgt ctgaattttc catgggtggg
     8641 gtatctacca ccaataatat ggaattgtgt gctgacgcca aaaaagtcca acggcagata
     8701 ttgcacgtgc ctggcttttg ggctgaatta tcctggcatt tacgccaacg tactacctca
     8761 aagacgaaag ccttatataa caaaagctga atataaggga aaacgatgca ggatttaagt
     8821 ggattctcgg tgccgaaagg gttccggggt ggcaacgcta ttaaagtgca attgtggtgg
     8881 gcagtacagg caacaatatt tgcctggtcg ccacaagtat tgtatcgctg gcgggcattt
     8941 ttattacgtt tattcggcgc aaaaatagga aaaaacgtag ttattcgtcc gtcagtaaaa
     9001 attacctatc cgtggaaatt aaccttaggt gattacgcgt gggtcggcga tgacgtcaat
     9061 ttatataccc tcggtgaaat aaccattggc gcacattcgg tgatatcgca aaaaagttat
     9121 ttatgcaccg ggagccacga ccatgcaagt caacatttca ccattaacgc cacgcctatt
     9181 gtgattggcg agaaatgctg gctggcgaca gatgtttttg ttgcccctgg cgtcacgatc
     9241 ggcgacggca ccgtcgtggg tgcgcgaagc agtgttttta aatcgcttcc ggcaaatgtg
     9301 gtttgccggg ggaatcccgc agtggtgata cgcgaacgcg ttgaaactga ataaattcaa
     9361 aaaatacaga ggaataagac atgtcaaaag tcgctctcat caccggtgta accggacaag
     9421 acggttctta cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc
     9481 gtcgtgcatc gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct
     9541 gcaacccgaa attccatctg cattatggcg acctgagtga tacctccaac ctgacgcgta
     9601 ttttgcgtga agtgcagccg gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg
     9661 tttcttttga gtcaccggaa tataccgcag acgtcgatgc gatgggtacg ctacgcctgc
     9721 tggaggcgat ccgcttcctc ggtctggaaa agaaaacccg tttctatcag gcttccacct
     9781 ctgaactgta cggtctggtg caggaaattc cgcagaaaga gaccacgccg ttctacccgc
     9841 gatctccgta tgcggtcgcc aaactgtacg cctactggat caccgttaac taccgcgaat
     9901 cctacggcat gtacgcctgt aacggtattc tcttcaacca tgaatccccg cgccgcggcg
     9961 aaaccttcgt tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt
    10021 cgtgcctgta cctcggcaat atggattccc tgcgtgactg gggccatgcc aaagactacg
    10081 taaaaatgca gtggatgatg ctgcaacagg aacagccgga agatttcgta attgctaccg
    10141 gcgttcagta ctccgtgcgt cagttcgtag aaatggcggc ggcacagttg ggtatcaaac
    10201 tgcgctttga aggcacgggc gttgaagaga agggcattgt ggtttccgtc accgggcatg
    10261 atgcgccggg cgttaaaccg ggcgatgtga ttatcgtcgt tgacccgcgt tacttccgtc
    10321 cggcggaagt tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga
    10381 aaccggaaat caccctcaga gagatggtgt ctgaaatggt ggctaatgac ctcgaagcgg
    10441 cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat
    10501 aagcatgagt aaacaacgca tttttatcgc tggccatcgc ggaatggtcg gttccgccat
    10561 caggcgacag ctcgaacagc gcggtgatgt ggagctggta ttacgcaccc gcgatgaact
    10621 gaacctgctg gacagccgcg cggtgcatga tttctttgcc agcgaaagca ttgaccaggt
    10681 ctatctggcg gcggcgaaag tgggcggcat tgttgccaac aacacctatc cggcggattt
    10741 catctatcag aacatgatga ttgagagcaa catcattcac gccgcgcatc agaacgacgt
    10801 gaacaaactg ctgtttctcg gatcgtcctg tatctacccg aaactggcaa aacagccgat
    10861 ggcagaaagc gagttattgc agggtacgct ggagccgacc aacgagcctt acgccattgc
    10921 caaaatcgcc gggatcaaac tgtgcgaatc ttacaaccgc cagtacggac gcgattaccg
    10981 ctcggtcatg ccgaccaacc tgtacgggcc gcacgacaac ttccatccga gtaactcgca
    11041 tgtgatccca gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt
    11101 ggtggtgtgg ggcagcggta cgccgatgcg cgaatttctg cacgtcgatg atatggcggc
    11161 ggcgagcatt catgtgatgg agctggcgca tgaagtctgg ctggagaaca cccagccgat
    11221 gctgtcgcac attaatgtcg gcacgggcgt tgactgcact atccgcgagc tggcgcaaac
    11281 catcgccaaa gtggtgggtt acaaaggtcg ggtggttttt gatgccagca aaccggatgg
    11341 cacaccgcgc aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat
    11401 ctcactggaa gcggggcttg ccagcactta ccagtggttc cttgagaatc aagaccgctt
    11461 tcgggggtaa tgatgttttt acgtcaggaa gactttgcca cggtagtgcg ctccactccg
    11521 cttgtctctc tcgactttat tgttgagaac agtcgcggcg agtttctgct tggcaaaaga
    11581 accaaccgcc cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa
    11641 acgctggaag ccgcatttga gcggctgacg atggcggaac tggggctgcg tctgccgata
    11701 acagcaggcc agttttacgg tgtctggcag cacttttatg acgataactt ctctggcacg
    11761 gatttcacca cgcactatgt ggtgctcggt tttcgcttca gagtcgcgga agaagatctg
    11821 ttactgccgg atgagcagca tgacgattac cgctggctga cgccggaagc gctgctcgcc
    11881 agtgacaatg ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta
    11941 cccggattat gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg
    12001 gcaaatacac cggagagatg gtggaatggc ttgcggcaca aggtcatgag gtgcgggtca
    12061 taaccgcacc gccttactac ccgcagtggc aggtgggcga gaactattcc gcctggcgct
    12121 acaaacgaga agagggggcc gccacggtgt ggcgctgccc gctgtacgtg ccaaaacagc
    12181 cgagcaccct gaaacgcttg ttgcatctcg gcagttttgc cgtcagcagt tttttcccgt
    12241 tgatggcgca acgtcgctgg aagccggatc gcattattgg cgtggtgcca acgctgtttt
    12301 gcacgccggg aatgcgcctg ctggcgaaac tctctggcgc gcgtaccgtg ctgcatattc
    12361 aggattacga agtggacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca
    12421 aagtggcaca gctggcaacg gcgttcgaac gtagcggact gcataacgtc gataacgtct
    12481 ccacgatttc gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg gcagaaaacg
    12541 tcatcttctt tcccaactgg tcagaaattg cccgttttca gcatgtcgca gatgtcgatg
    12601 ttgatgccct tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttactccg
    12661 gcaatattgg tgaaaagcag gggctggaaa acgttattga agctgccgat cgcctgcgcg
    12721 atgaaccgct gatttttgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa
    12781 tggcgcagca gcgtggactg cgcaacatgc aatttttccc gctgcaatcg tatgacgctt
    12841 tacccgcact gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag
    12901 atgccgtatt gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac gcggtgatta
    12961 ccgcagaggc ccacacagaa ctggggcagc tttgcgaaac ctttccgggc attgcggttt
    13021 gcgtagagcc ggaatcggtc gaggcgctgg tggcggggat ttgccaggcg ctcctgctgc
    13081 ccaaacacaa cacggtggca cgtgaatatg ccgaacgcac gctcgataaa gagaacgtgt
    13141 tacgtcaatt tataaatgat attcggggat aattatggcg cagtcgaaac tctatccagt
    13201 tgtgatggca ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttatcccaa
    13261 gcagttttta tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa
    13321 cggcgtggag tgcgaaagcc ctgtggtgat ttgcaatgag cagcaccgct ttattgtcgc
    13381 ggaacagctg cgtcaactga acaaactcac cgagaacatt attctcgaac cggcagggcg
    13441 taacactgca cccgccattg cgctggcggc gctggcggca aaacgtcata gcccggagag
    13501 cgacccgtta atgctggtgc tggcggcgga tcatgtgatt gccgatgaag acgcgttccg
    13561 tgccgccgtg cgtaatgcta tgccatatgc cgaagcgggc aggctggtga ccttcggcat
    13621 tgtgccggat ctacctgaaa ccggatatgg ctatattcgt cgcggtgaag tgtctgcggg
    13681 tgagcaggat acggtggcct ttgaagtggc gcagtttgtc gaaaaaccaa atctggaaac
    13741 cgcgcaggcc tatgtagcaa gcggcgaata ttactggaac agcggtatgt tcctgttccg
    13801 tgccggacgc tatctcgaag aactgaaaaa atatcgcccg gatatcctcg atgcctgcga
    13861 aaaagcgatg agcgccgtcg atccggatct cgattttatt cgcgtggatg aagaagcctt
    13921 tctggcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgccgt
    13981 tgtggtgccg atggatgcgg gctggagcga tgtcggttcc tggtcttcat tatgggagat
    14041 cagcgcccac accgccgagg gcaacgtttg ccacggcgat gtgattaatc acaaaactga
    14101 aaacagctat gtgtatgccg aatctggcct ggtcaccacc gtcggggtga aagatttggt
    14161 ggtagtgcag accaaagatg cagtgctgat tgccgaccgt aacgcggtgc aggatgtgaa
    14221 aaaagtggtc gagcagatca aagccgacgg tcgccatgag catcgggtgc atcgtgaagt
    14281 gtatcgtccg tggggcaaat atgactctat tgatgcgggc gaccgctacc aggtgaaacg
    14341 catcaccgtg aaaccgggcg agggcttgtc ggtacagatg catcatcacc gcgcggaaca
    14401 ctgggtggtg gtcgcaggaa cggcaaaagt cactattgac ggtgatatca aactgcttgg
    14461 tgaaaacgag tccatttata ttccgctggg ggcgacacat tgcctggaaa acccggggaa
    14521 aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt
    14581 gcgcttcgcg gatcgctacg ggcgggtgta gtagttgaat aatcaaccgg attaattctc
    14641 tggtaaataa cgacaaaaac aaaattggcc gcttcggtca gggccaacta ttgcctgaaa
    14701 aagggtaacg acatgaaaaa attaacctgc tttaaagcct atgatattcg cgggaaatta
    14761 ggcgaagaac tgaatgaaga tattgcctgg cgtattggtc gcgcctatgg cgaatttctt
    14821 aaaccgaaaa ccattgtttt aggcggtgat gtccgcctca ccagcgaaac cttaaaactg
    14881 gcgctggcaa aaggtttaca ggatgcgggc gtcgatgtgc tggatattgg cacgtccggc
    14941 actgaagaga tctatttcgc cacgttccat ctcggcgtgg atggcggcat cgaagttact
    15001 gccagccata atccgatgga ttacaacggc atgaaactgg tgcgcgaagg ggctcgcccg
    15061 atcagcggtg ataccggact gcgcgatgtc cagcgtctgg cagaagccaa tgacttccct
    15121 cctgtcgatg aaaccaaacg cggtcgctat cagcaaatca atctgcgtga cgcttacgtt
    15181 gatcacctgt tcggttatat caacgtcaaa aaccttactc cgctgaaact tgtgatcaac
    15241 tccgggaacg gcgcggcggg tccggtagtt gacgccatcg aagcccgctt taaagccctc
    15301 ggcgcaccgg tggagttaat caaagtgcat aacacgccgg acggcaattt ccccaacggt
    15361 attcctaacc cgctgctgcc ggaatgccgc gacgacaccc gcaatgcggt catcaaacac
    15421 ggcgcggata tgggcattgc ttttgatggc gattttgacc gctgtttcct gtttgacgaa
    15481 aaagggcagt ttattgaggg ctactacatt gtcggcctgc tggcagaagc attcctcgaa
    15541 aaaaatcccg gcgcgaagat catccacgat ccacgtctct cctggaacac cgttgatgtg
    15601 gtgactgccg caggcggcac tccggtgatg tcgaaaaccg gacacgcctt tattaaagaa
    15661 cgtatgcgca aggaagacgc catctacggt ggcgaaatga gcgcccacca ttacttccgt
    15721 gatttcgctt actgcgacag cggcatgatc ccgtggctgc tggtcgccga actggtatgc
    15781 ctgaaaggaa aaacgctggg cgaactggta cgcgaccgga tggcggcgtt tccggcaagc
    15841 ggtgagatca acagcaaact ggcgcaaccc gttgaggcga ttaaccgcgt ggaacagcat
    15901 tttagccgtg aggcgctggc ggtggatcgc accgatggca tcagcatgac ctttgccgac
    15961 tggcgcttta acctgcgttc ctccaacacc gaaccggtgg tgcggttgaa tgtggaatcg
    16021 cgcggtgatg tgccgctaat ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag
    16081 taatgtcgga tcttccctta ccccactgcg ggtaaggggc taataacagg aacaacgatg
    16141 acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa
    16201 cgcttttcag atatcaccat catgtttgcc ggactatggc tggtttgcga agtgagcggc
    16261 ctgtcattcc tctacatgca cctgttggtg gcgctgatta cgctggtggt gttccagatg
    16321 ctgggtggca tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc gacagaattt
    16381 gccctgctgc tacaaaactg gaccttaagc gtgattttca gcgccggact ggtggcgttc
    16441 aacaatgatt tcgatacgca actgaaaatc tggctggcgt ggtatgggct gaccagcatc
    16501 ggactggtgg tttgccgttc atgtattcgc attggggcgg gctggctgcg taatcatggc
    16561 tataacaagc ggatggtcgc ggtggcgggg gatttagccg ccggacaaat gctgatggag
    16621 agcttccgta atcagccgtg gttagggttt gaagtggtgg gcgtatacca cgacccaaaa
    16681 ccgggcggcg tttctaacga ctgggcgggc aatctgcaac agctggtcga ggatgcgaaa
    16741 gcgggcaaga ttcataacgt ctatatcgcg atgcaaatgt gcgacggcgc acgagtgaaa
    16801 aaactggtcc atcaactggc ggataccacc tgttcggtgc tgctgatccc cgatgtcttt
    16861 accttcaaca ttctccattc acgtctcgaa gagatgaacg gtgttccggt ggtgccgctt
    16921 tacgacacgc cgctttccgg ggttaaccgc ctgctcaaac gtgcggaaga tattgtgctg
    16981 gcgacgctta ttctgctgct gatctccccg gtgctgtgct gtattgctct ggcggtaaaa
    17041 ctcagttcac cagggccggt tatttttcgc cagactcgct acggcatgga tggcaaaccg
    17101 atcaaagtgt ggaagttccg ttcgatgaaa gtgatggaga acgacaaagt ggtaactcag
    17161 gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg
    17221 gatgaattgc cgcagtttat caatgtgctg actggcggga tgtcgattgt cggtccacgt
    17281 ccacacgcgg tggcgcataa cgaacagtat cgccagctca ttgaaggcta catgctgcgc
    17341 cataaggtga aaccgggcat taccggctgg gcgcagatta atggctggcg tggcgaaacc
    17401 gacacgctgg agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg
    17461 agcgtctggt tcgatatcaa aatcgttttc ctgacggtat tcaagggttt cgttaacaaa
    17521 gcggcatatt gatatgagct tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat
    17581 tgccacggtg atcatcatcg gcctcgggct ggtgcagatg accgtgctgg cgcggattat
    17641 cgacaaccac cagttcggtt tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac
    17701 gctttctgac ttcggcatcg ctaactcgat tattcagcga aaagaaatca gccaccttga
    17761 actcaccacg ctgtactggc tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt
    17821 tttgttgagt gatctcatcg gcgacgtgct gaataacccg gacctggcac cgttgattaa
    17881 aacattgtcg ctggcgtttg tggtgatccc ccacgggcag cagttccgcg cgttgatgca
    17941 gaaggagctg gagttcaaca aaatcggcat gatcgaaacc agcgcggtgc tggcgggctt
    18001 cacttttacg gtggttagcg cccatttctg gccgctggcg atgaccgcga tcctcggtta
    18061 tctggtcaat agtgcggtaa gaacgctgct gtttggctac tttggtcgca aaatttattg
    18121 ccccggtctg catttctcgc tggcgtcggt ggcaccaaac ttacgttttg gtgcctggct
    18181 gacggcggac agcatcatca actatctcaa taccaacctt tcaacgctgg tgctggcgcg
    18241 tattctcggc gcgggcgtgg cagggggata caacctggcg tacaacgtgg ccgttgtgcc
    18301 accgatgaag ctgaacccaa tcatcacccg cgtgttgttt ccggcattcg ccaaaattca
    18361 ggacgacacc gaaaagctgc gcgttaactt ctacaagctg ctgtcggtag tggggattat
    18421 caactttccg gctctgctgg ggctgatggt ggtgtcgaat aactttgtac cgctggtctt
    18481 tggtgagaag tggaacagca ttattccggt gctgcaattg ctgtgtgtgg tgggtctgct
    18541 gcgctccgta ggtaacccga ttggttcgct gctgatggcg aaagcgcggg tcgatatcag
    18601 ctttaaattc aacgtattca aaacatttct gtttattccg gcgattgtta tgggtgggca
    18661 gatggcgggc gcaatcggcg tcacgctcgg tttcctgctg gtgcagatta tcaacaccat
    18721 tctgagttac ttcgtgatga ttaaaccggt tcttggttcc agttatcgcc agtacatcct
    18781 gagtttatgg ctgccgtttt atctctcgct gccgacgctg gtggtcagtt atgcgctggg
    18841 cattgtgctg aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa tagccgcggg
    18901 cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat ccgctggtgg tggaggtgaa
    18961 gcgtcagttt tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt gagttgttgt
    19021 tttcggttcc ggcaacgaaa acaacaacat aacctcacct ggcatcaaat ttatgaggaa
    19081 aaaatgaaat tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc
    19141 ttgcgcggct tacttgatgc catcaacatc ctcaatccac acgcagaagt ggacgtgatg
    19201 agccgctatc cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg
    19261 ttcctgcaaa tgaaacaaca caacagcgca gcgggcgttg tcgggcgcgt taaaaaagtc
    19321 ctccgtcgcc gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg
    19381 cgtaatatcg ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac
    19441 gccattattc aggtcggcgg atcgtttttt gtcgatctct acggtgtgcc gcagtttgaa
    19501 cacgcgcttt gcactttcat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc
    19561 ccgttccagg acgagcaatt taaccaactg gcgaaatacg tattcggtcg ctgcgacgcg
    19621 ctgatcctgc gcgaatcggt cagccttgat ctgatgaaac gcagcaatat caccactgca
    19681 aaagttgaac atggcgtcga taccgcgtgg ctggtcgatc accacacaga agacttcacc
    19741 gccagctatg ctgttcaaca ctggctggat gttgccgcac aacagaaagc ggtggcaatt
    19801 accctgcgcg aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa
    19861 aaagcctttg ccggggtggt caatcgcatt ctcgacgaag ggtatcaggt gattgcgctc
    19921 tccacctgta cgggcatcga cagctataac aaagacgacc gcatggtggc gctcaatctt
    19981 cgccagcaca tcagcgatcc tgcccgttac cacgtggtga tggatgaact caacgatctg
    20041 gaaatgggca aaatcctcgg tgcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc
    20101 attatctcga tgaattttgc tactccggca attgccatca actacgaaca taaatccgcc
    20161 gggattatgc aacaactggg gctaccagag atggcaattg atatccgtca tttattagac
    20221 ggcagcctgc aagcgatggt tgcggatacc ttaggccagc ttccggcact gaacacgcga
    20281 cttaacgaag ccgtcagtcg cgaacgtcag acgggaatgc agatggtgaa atctgtgctt
    20341 gaacgcatcg gggaggcgaa atgaaggtcg gcttcttttt actgaaattt ccactgtcgt
    20401 cggaaacctt cgtcctcaat caaattaccg cgtttattga tatgggcttt gaggtggaga
    20461 ttgtcgcgct gcaaaaaggc gacacccaga acacccacgc ggcatggacg aaatacaacc
    20521 ttgccgccag aacccgctgg ttacaggacg aacctacggg caaagtggcg aaactgcgcc
    20581 accgcgccag ccagacctta cgcggcattc atcgtaaaaa tacctggcag gcgctcaacc
    20641 tcaaacgcta tggtgctgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg
    20701 caacaccgtt tcatgctgat gtgtttatcg cacattttgg ccctgcgggg gtaaccgcag
    20761 caaaactacg ggaactgggt gtcattcgcg gcaaaatcgc cactatcttc cacggtattg
    20821 atatctccag tcgggaagtg ctcaaccatt acactcccga atatcaacaa ctgtttcgcc
    20881 gtggcgacct gatgttaccg attagcgatc tgtgggccgg aaggctgcaa aaaatgggct
    20941 gcccgaggga aaaaattgcc gtatcgcgca tgggtgtaga catgacgcgc tttagcccgc
    21001 gtccggtgaa agcgcctgca acgccgctgg aaatcatttc cgtcgcacgc ttaaccgaga
    21061 aaaaaggcct gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat
    21121 ttcgctatcg catcctcggc attggcccgt gggaacgacg cctgcgcacg ctcatcgaac
    21181 aatatcaact ggaagatgtg gtagagatgc cgggctttaa accaagccac gaagtgaaag
    21241 agatgctcga tgacgcggat gtcttcctgt tgccatcggt tacaggtgcg gatggcgata
    21301 tggaaggtat tccggtggcg ctaatggaag cgatggcggt cggcattccg gtggtttcta
    21361 ctctgcatag cggaataccg gaactggtgg aggccgataa atccggctgg ctggtgcctg
    21421 agaacgatgc tcgcgcactg gcgcaacgac tggcggcgtt tagccaactg gacaccgacg
    21481 aacttactac ggttgtcaaa cgtgcgcgcg aaaaagtcga acacgatttt aaccagcagg
    21541 tgatcaatcg agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt
    21601 aaaaaactct cccggcgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc
    21661 cctttcgctc gcgcgcttcc cgcccgacaa atcgttaaca ttaacgacta caacccacac
    21721 gactggatcg cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc
    21781 ggattcgttt gtgaaaatat caacaccggc atcttcattc ctcctggcaa aacgttgcac
    21841 atccttggaa gcctgcgcgg caacggcaga gggcgttttg tcttacaaga cggcagccag
    21901 gtgacagggg agaagggcgg ccgtatgcat aacatcaccc tggatgtgcg cggttctgac
    21961 tgcatcatca aagggttggc gatgagcggt tttggcccgg taacgcagat ttatatcggc
    22021 ggcaaaaata aacgcgtcat gcgcaacttg accatcgata acctgaccgt cagccacgct
    22081 aattacgcca ttttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat
    22141 tgtaagttca gcgacttaca gggcgacgcc attgaatgga atgtagcaat caatgatcgc
    22201 gacattttga tatccgacca tgtcatcgag cgcatcaact gtactaacgg caaaatcaac
    22261 tggggtattg gcataggact ggcaggaagt acttacgata ataattatcc ggaaaaccag
    22321 gcagtgaaaa actttgtcgt tgcgaatatc acgggatcag attgtcggca actgatccat
    22381 gttgaaaatg gtaaacattt tgttatccgt aatatcaaag ctcgtaatat cacgccggat
    22441 ttcagtaaga aagcaggtat tgataacgcc acagttgcta tttatggttg tgacaacttt
    22501 gtgattgata atattgaaat gattaatagt gccgggatgt taatcggcta tggggtaatt
    22561 aaaggcaaat atctctcgat accgcagaat ttccgagtga atgatattca actggataat
    22621 actcatcttg cttataaatt gcgcggcatc caaatctccg ccgggaatgc tgtctccttt
    22681 gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
    22741 catcttttta tgcgaaatat caatgtgatg caggaatcct cagttggacc cgcattgagc
    22801 atgaactttg acatgcgcaa agacgttcgc ggcgtgttta tggcgaaaaa agaaacactg
    22861 ctgtctcttg caaatgttca tgcggtgaat gaaaaaggac aaatctccgt cgatatcgac
    22921 aggattaatc accatattgt taatgtggag aagattaact ttagattgcc agaacggagg
    22981 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
    23041 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat
    23101 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
    23161 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgtatat gttgcctgcc
    23221 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatc
    23281 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcttccaag
    23341 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
    23401 gtgaagcgtc aactgctggc ggaagtacag tccatttgcc cgccgggcgt gacaattatg
    23461 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttttatgtgc acgacctgcc
    23521 attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc
    23581 gacccgctgc gctacaacct tgctgccatg attgcgcgct tcaacgaaac gggccgcagc
    23641 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actctgtcat ccagaccaaa
    23701 gagccgctgg accgcgaagg taaagtcagc cgcattgttg aatttatcga aaaaccggat
    23761 cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat
    23821 atttggccgg aactggaacg cacgcagcct ggtgcatggg ggcgtattca gctgactgat
    23881 gccattgccg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac cggcgacagc
    23941 tacgactgcg gtaaaaaaat gggttatatg caagcgttcg tgaagtatgg actacgcaac
    24001 ctcaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa
    24061 tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt agcggcgaaa
    24121 gtaatttgtt gcgaattttc ctgccgttgt tttatataaa caatcagaat aacaacgact
    24181 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
    24241 aagacaatta gcgcttgaat ttttcgagtt aagcgcgagt gggtaacgct cgtcacatcg
    24301 taggaatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac
    24361 ctctattaat caaactgaga gccgcttatt tcacagcatg ctctgaagta atatggaata
    24421 aattaagtga agatacttgt tactgggggc gcaggattta ttggttctgc tgtagttcgt
    24481 cacattataa ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga
    24541 aacctggaat cacttgctga tgtttctgac tctgaacgct atgtttttga acatgcggat
    24601 atttgcgatg ctgctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg
    24661 cacctggctg ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa
    24721 accaatattg ttggtactta tgtccttttg gaagcggctc gcaattactg gtctgctctt
    24781 gatggcgaca agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt
    24841 gatttgcctc atcctgacga agtaaataat aaagaacaat tacccctctt tactgagacg
    24901 acagcttacg cgcctagtag tccttattcc gcatcaaaag catccagcga tcatttagtc
    24961 cgcgcgtgga aacgtaccta tggtttaccg actattgtga ctaactgttc gaataactac
    25021 ggtccttatc actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt
    25081 aaggcattac ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat
    25141 catgcgcgtg cgttatatac cgtagttact gaaggtcaag cgggtgaaac ctataacatt
    25201 ggcggacaca acgaaaagaa aaacatcgat gttgtgctga ctatttgtga tttgttggac
    25261 gagatagtcc cgaaagagaa atcttatcgt gagcaaatta cttatgttgc tgatcgccca
    25321 gggcatgatc gccgttatgc gatcgatgcc gataaaatta gccgtgaatt gggctggaaa
    25381 ccgcaagaaa cgtttgagag cgggattcga aagacggtgg aatggtacct gtccaataca
    25441 aaatgggttg ataatgtgaa aagtggcgcc tatcaatcgt ggattgaaca gaactatgag
    25501 ggccgccagt aatgaatatc ctcctattcg gcaaaacagg gcaggtaggt tgggaactac
    25561 agcgtgctct ggcaccgctg ggtaatctga ttgctcttga tgttcactcc actgattatt
    25621 gtggtgattt tagtaatccc gaaggtgtag ctgaaactgt caaaaaaatt cgccctgatg
    25681 ttattgttaa tgcggctgct cacactgcag tagacaaagc ggaatcagaa ccggaatttg
    25741 cacaattact taacgcgaca agtgtcgaag cgattgcaaa agcagccaat gaagtcggcg
    25801 cttgggttat tcactactct actgactacg tatttccggg aaccggtgaa ataccgtggc
    25861 aggagacgga tgctacagca cctttgaatg tttatggtga aactaaacta gccggagaaa
    25921 aagcgttaca gaaacattgc gcgaagcata ttattttccg taccagctgg gtctatgcag
    25981 gtaaaggaaa taacttcgcc aaaacaatgt tgcgtctggc aaaagagcgc gaagaactgg
    26041 ctgtgataaa cgatcagttt ggcgcaccaa caggtgccga attgctggct gattgcaccg
    26101 ctcatgccat tcgcgtagca gtagacaaac cagaagttgc tggtttgtac catctggttg
    26161 ctggcggtac aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcaaag
    26221 cagggattaa ccttgctctt aacaaactta acgccgtgcc aacaacggcc tatcccacac
    26281 cagcccgtcg accccataac tctcgcctca atacagaaaa gtttcagcag aactttgcgc
    26341 ttgtcttgcc tgactggcag gtgggcgtga aacgaatgct caacgaatta tttacgacta
    26401 cggcaattta acaaattttt gcatctcgct catgatgcca gagcgggatg aattaaaagg
    26461 aatggtgaaa tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt
    26521 tatcctgtga cgatggcagt aagtaaacaa ctgctgccga tttatgataa acctatgatc
    26581 tattacccgc tctcaacact gatgctggcg ggtattcgcg atattcttat tatcagtacg
    26641 ccacaggata caccgcgttt ccaacaattg ttgggggacg ggagccagtg ggggcttaat
    26701 ctacagtata aagtacaacc gagtccggat ggcctggcac aagcgtttat cattggtgaa
    26761 gactttattg gtggcgatga ttgtgcactc gtgcttggag ataatatctt ctatggacac
    26821 gacttgccaa aattaatgga cgctgcggtt aacaaagaaa gcggtgcaac ggtatttgct
    26881 tatcacgtta atgatcctga acgctatggt gtcgtggagt ttgataataa cggtacggca
    26941 attagcctgg aagaaaaacc gctggaacca aaaagtaact atgcggttac tgggctttat
    27001 ttctatgaca atgacgtcgt ggaaatggcg aaaaacctta agccttctgc ccgtggcgaa
    27061 ctggaaatta ccgatattaa ccgtatttat atggaacaag gacgtttgtc tgtcgctatg
    27121 atggggcgtg gttatgcatg gctggataca gggacgcatc aaagccttat tgaagcaagt
    27181 aacttcattg ccaccattga agagcgtcag ggattaaaag tatcttgccc ggaagagatt
    27241 gcttatcgta aagggtttat tgatgctgag caggtaaaag tattagccga accgcttatc
    27301 aagaatcaat atggtcaata tttgctgaaa atgatcagcg aatagtatgt gggaactcaa
    27361 tgatggatat taaattaatc tctttgcaaa aacatgggga tgagcgtggt gcattaattg
    27421 ctcttgaaga gcaacgaaat atacctttcg aagtcaaaag aatatattac atactcgaga
    27481 ctcttaaggg agtaagacgc ggatttcatg cgcacaaggt tactcgtcag ttagctattg
    27541 tagtcaaggg agcttgtaaa tttcatctgg ataatggtaa agagacaaag caggtggaac
    27601 ttaatgatcc aacaattgcg ttgctgatag aaccctatat atggcatgaa atgtatgatt
    27661 ttagtgatga ttgtgtgctg cttgtaattg cggatgattt ctataaagag tctgattata
    27721 tacgcaatta tgatgatttt attagaagag taaattcaat tgagaattca taagctaagt
    27781 gacgtccaga caacatcaat tggtgatgga acaactatct ggcagtttgt tgtgatacta
    27841 aaaggtgctg taattggtaa taattgcaat atctgtgcaa ataccttgat tgaaaataac
    27901 gttgtaattg gtaacaatgt cacagtcaaa agcggtgtgt atatttggga tggcgttaaa
    27961 atagaggata atgtttttat tggtccttgt gtagcattta caaatgataa gtatcctcgc
    28021 tctaaagtct atcctgatga atttttgcac acaataatac gcaaaggagc atcaataggt
    28081 gctaatgcaa ccatcctgcc aggaattgaa attggtgaaa aagcaatcgt tggtgcgggg
    28141 agtgtcgtaa ccaaaaatgt accgccatgc gcaatagtag taggtaatcc agctcgattt
    28201 attaaatggg tagaggataa tgaataaaat tgatttttta gatctttttg caattaacca
    28261 gcgacagcac aaagaattag tttctgcgtt tagtagggtg ctagattctg gttggtatat
    28321 catgggcgaa gaacttgagc agttcgagaa agagttcgca gaatactgtg gagttaagta
    28381 ttgcattggt gtagcaaatg gccttgatgc gttgatacta gtattgaggg catggaaaga
    28441 actcggctat ctagaggacg gtgacgaggt attagtaccg gcaaatacat atattgcttc
    28501 tattcttgct ataacagaga acaaacttgt tcctgttctt gttgaaccag atatagaaac
    28561 ttataatatt aatcctgctt taattgaaaa ttacattacg gaaaaaacca aagcaatatt
    28621 accggttcac ttatatggtc tattgtgcaa tatgccagaa attagtgcaa tcgcaagaaa
    28681 atataatctg ttgattcttg aagattgtgc acaagcacat ggtgcaatac gtgatggtcg
    28741 caaagctgga gcttgggggg atgctgcagg atttagtttt tatccaggaa aaaaccttgg
    28801 agctttgggg gatgcgggag ctgttactac aaataatgca gaattatcct caactataaa
    28861 agctttgcga aattatgggt cacataagaa atatgaaaat atttatcagg gattgaatag
    28921 tcgattggat gaactgcaag cagccttatt gcgtgtaaaa atccatacat taccggaaga
    28981 tactgcgatt cggcaaagga ttgctgaaaa atatattcgt gaaataaaaa accctgcgat
    29041 tacgttacca gtgtacgaag gccaaggtgc gcacgtttgg catttatttg tagtaagaat
    29101 cgctaatcgt gaaaaattcc agtcatactt attagagaag ggtatccaaa ccttaattca
    29161 ctatccatta ccaccccata agcagcaagc atatcaaaat atgtctagtc ttagccttcc
    29221 aattactgag caaattcatg atgaagtcat ttctttacct ataagtccgg taatgagtga
    29281 agatgatgtc aattatgtaa tcaaagtggt caatgattac aagtaatgaa aaaatttctt
    29341 caggtaacta tattatccgc tatctataca ttcattaaaa tgattgcggg ttttatcatc
    29401 ggtaaggtag tagcaattta tacagggcca tcaggggtag caatgcttgg ccaagtgcaa
    29461 agtttaatca caatagttgc aggtactacc tctgcacctg taagcactgg tcttgttcga
    29521 tatactgcgg aaaattggca agaaggacag gaagcatgcg cgccatggtg gcgcgcatgc
    29581 ttaagggtta ctctgttttt attcttgctt attattcccg ttgttatttt attgtcgaaa
    29641 aatattagcg agttactttt tagcgatgga caatacacat ggttaatcat tttcgcatgt
    29701 tgtatattgc cattctccat tttaaataca ttgatcgct
//
LOCUS       DARNVM010000044        29647 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.044, whole genome shotgun sequence.
ACCESSION   DARNVM010000044 DARNVM010000000
VERSION     DARNVM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.044"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            152..1735
                     /locus_tag="SI621_001767"
     CDS             152..1735
                     /locus_tag="SI621_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEI4064495.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RVVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(2187..4040)
                     /gene="asmA"
                     /locus_tag="SI621_001768"
     CDS             complement(2187..4040)
                     /gene="asmA"
                     /locus_tag="SI621_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HEI4064496.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRINRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEQPEWQLRLQFPQLNLDNLIPLNETANGENGTAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4062..4643)
                     /gene="dcd"
                     /locus_tag="SI621_001769"
     CDS             complement(4062..4643)
                     /gene="dcd"
                     /locus_tag="SI621_001769"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEI4064497.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4735..5376)
                     /gene="udk"
                     /locus_tag="SI621_001770"
     CDS             complement(4735..5376)
                     /gene="udk"
                     /locus_tag="SI621_001770"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEI4064498.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5694..9011
                     /locus_tag="SI621_001771"
     CDS             5694..9011
                     /locus_tag="SI621_001771"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEI4064499.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNNTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAATHSERAA
                     ISLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9049..9906)
                     /gene="alkA"
                     /locus_tag="SI621_001772"
     CDS             complement(9049..9906)
                     /gene="alkA"
                     /locus_tag="SI621_001772"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HEI4064500.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLSMTIPGDVEQAMKMLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10040..11392
                     /gene="yegD"
                     /locus_tag="SI621_001773"
     CDS             10040..11392
                     /gene="yegD"
                     /locus_tag="SI621_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEI4064501.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLV
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11405..13345)
                     /gene="yegI"
                     /locus_tag="SI621_001774"
     CDS             complement(11405..13345)
                     /gene="yegI"
                     /locus_tag="SI621_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HEI4064502.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQSTLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATIHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYTYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGMATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQTEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRDLAALQDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKVALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPVDRQAVMAKMTAKRHQLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSCC"
     gene            complement(13342..14103)
                     /gene="pphC"
                     /locus_tag="SI621_001775"
     CDS             complement(13342..14103)
                     /gene="pphC"
                     /locus_tag="SI621_001775"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HEI4064503.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDDDAVSRLETYTSTERAHKVAAFTDGIQRLALNMLNNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14100..14759)
                     /locus_tag="SI621_001776"
     CDS             complement(14100..14759)
                     /locus_tag="SI621_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEI4064504.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVCQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14980..15039)
                     /gene="ibsA"
                     /locus_tag="SI621_001777"
     CDS             complement(14980..15039)
                     /gene="ibsA"
                     /locus_tag="SI621_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HEI4064505.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15312..15368)
                     /locus_tag="SI621_001778"
     CDS             complement(15312..15368)
                     /locus_tag="SI621_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEI4064506.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15640..15696)
                     /locus_tag="SI621_001779"
     CDS             complement(15640..15696)
                     /locus_tag="SI621_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEI4064507.1"
                     /translation="MMKWIIIVVLLVISYPAY"
     gene            15975..17222
                     /gene="mdtA"
                     /locus_tag="SI621_001780"
     CDS             15975..17222
                     /gene="mdtA"
                     /locus_tag="SI621_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HEI4064508.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKTGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17222..20344
                     /gene="mdtB"
                     /locus_tag="SI621_001781"
     CDS             17222..20344
                     /gene="mdtB"
                     /locus_tag="SI621_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HEI4064509.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20345..23422
                     /gene="mdtC"
                     /locus_tag="SI621_001782"
     CDS             20345..23422
                     /gene="mdtC"
                     /locus_tag="SI621_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HEI4064510.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGIKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLIGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23423..24838
                     /locus_tag="SI621_001783"
     CDS             23423..24838
                     /locus_tag="SI621_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064511.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LRMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTVPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLIATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24835..26238
                     /gene="baeS"
                     /locus_tag="SI621_001784"
     CDS             24835..26238
                     /gene="baeS"
                     /locus_tag="SI621_001784"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HEI4064512.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAGQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26235..26957
                     /gene="baeR"
                     /locus_tag="SI621_001785"
     CDS             26235..26957
                     /gene="baeR"
                     /locus_tag="SI621_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HEI4064513.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27137..27469
                     /locus_tag="SI621_001786"
     CDS             27137..27469
                     /locus_tag="SI621_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEI4064514.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27617..28978
                     /gene="yegQ"
                     /locus_tag="SI621_001787"
     CDS             27617..28978
                     /gene="yegQ"
                     /locus_tag="SI621_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEI4064515.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTSERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29320..29637)
                     /locus_tag="SI621_001788"
     CDS             complement(29320..29637)
                     /locus_tag="SI621_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064516.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6844 a         7912 c         7828 g         7063 t
ORIGIN      
        1 tattattacc acttttaatt cagggataat gtgaggttat aaacctcaaa taatgtgaga
       61 taaatagtgc cgcaacattg catttttgcc ctgatttatc catgatcgaa ttgtgacatt
      121 tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc cgtctatctg
      181 ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata acctggtctt
      241 tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac gggttaccgg
      301 gctgctgctg gcgatgttaa tgcgcctgtt actgctggcg tcaatctcct ggctggtcac
      361 cctgacacaa ccgctattca gctttcgctc gtttaccttt agcgcccgcg acttaatcat
      421 gctgtttggt ggtttcttcc tgctgttcaa agccacgatg gagctgaacg aacggctgga
      481 ggggaaagac agcaataacc ccacgcaacg caaaggtgcg aagttctggg gggtggtgac
      541 acaaattgtg gtactggacg ccatcttctc actcgactcg gtgattaccg ccgtcgggat
      601 ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc tgatgttgat
      661 ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta ttctctgctt
      721 aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct tcgtcattcc
      781 gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcgc tcaatcagtt
      841 ggctatcttt aaccgtcgac gttttctttc cgctaaccag acactgcgcc agcggactac
      901 cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg acgccgaaac
      961 cgcgtccatg ctggtggatc atggtaacca gcagatcttt aatccgcagg aacggcggat
     1021 gattgagcgg gtacttaatc ttaatcagcg taccgtcagc agcattatga cgtcgcgcca
     1081 cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt tgctggagcg
     1141 aaatcagcat acgcgggtgg tggttaccga cggtgatgac gcagaagatt tgctcggtgt
     1201 tgttcacgtt atcgacctgc tacaacagtc tctgcgcggc gaaccgctca acctgcgggt
     1261 gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttacctg ccctggagca
     1321 gttccgtaat gcccgcacgc actttgcttt tgtagtggat gagtttggct cggtggaagg
     1381 gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga acgaagtgga
     1441 agagatcgac gcccgccatg atattcagaa aaatgcggac ggttcctgga cggcgaatgg
     1501 tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg aaaaacgtga
     1561 atatcacacc attgccgggc tattgatgga atatttgcag cgtattccaa aacctggcga
     1621 agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc atcgcgtgca
     1681 gaaggtgcag attattccgc tgcataagga tggcgagatg gaatacgagg tgtgatggta
     1741 tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa gcgttgtctg
     1801 tggtacatgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga atcgtttcct
     1861 gtaggccggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg gatgttacct
     1921 cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag cggcgattta
     1981 tcgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca cgttcaccgg
     2041 cttgagcgtt ccatagtaac gaggaacatc aagtgaacgg aacagccttc gatcttacat
     2101 attcagaata taatggtgcc gccacgaacg cctgatgcga cgctttacgc gtcttatcag
     2161 gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt cacatctttg
     2221 ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt cgcttcgtcc
     2281 tgtagatgtt tgcgcagtaa ctgatccact tgcagactgt aattgagttg ctgccagttg
     2341 ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt gctttccccg
     2401 ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc tgccagattc
     2461 aacatacctt cccctgtaag cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc
     2521 acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagacgcgt tacgttatcg
     2581 aagttttcag cggccttcac atcaccacca ttacgttcta ccgcttgctg aatcatctgc
     2641 tggaagttca tcccttccat gcgcgtgtcg gtcatttcga catgtgcctg tccttgccag
     2701 ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc cagtgacatt
     2761 tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat ctcaacgttt
     2821 tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag cgtgcccggc
     2881 agtgaaacct gtccaccgtt aagtttgccc tgcagttgag taatttccag cagacccgac
     2941 ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg cacgttactg
     3001 gcctgcaaca aaatatcagc cgtaaagcct tgcagtccct gataggccgg ttcatcaata
     3061 cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccttgctg cgcggtaccg
     3121 ttttcaccgt tcgctgtttc attaagcggg atgaggttgt caagattcag ttgcggaaat
     3181 tgcagtctca gctgccattc cggttgctct gtcagcgtga cctgtgcttg cccgctcagc
     3241 gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg tgactcctgc
     3301 cactgggcct ggaaactccc ctgcccctga ataccttgtt ttggtaaatc tgcaccctgc
     3361 aactgccagt taacttgttc aatagccgcc gttaaatcat gcggataatc agaagcatct
     3421 accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaatccg cccggagaac
     3481 tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt gcggattgtc
     3541 acctgctcgt catcttcatg ctggaacacc agcacgctgt ccgccacctt aagactggat
     3601 atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc gcgtggtgca
     3661 accggggcat cttcactgcg taccgcttcc gtctgcggcg tcagttggat cactgcccct
     3721 tttagcatca cctgcttaac gctcagttga tgactcagca gtggtaaaag cgccacgtcc
     3781 agacgcatgt tgtctgcgcg aaccagtggc tggctcgcgc cctgggcggt gagagacatt
     3841 cgcccggaga ggatactaag ttgcggccag acgtgccaac gcagtggccc gtcgagctgc
     3901 aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa atcattcgga
     3961 ttcaccagca acactaacgc agataacccg gccaccagca cgaccaggag tatcatcagc
     4021 gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt atcgattcgg
     4081 ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc
     4141 gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc
     4201 gccagcggca gcttaccgga gttgtagaac tccagcacaa tacagccaga ccagcccgga
     4261 tcgatacggt gcgcggtgac gtgcaccata agccccagac gcgccagtga ggaacgaccg
     4321 tccagccatc ccaccagatc ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc
     4381 agctctcctg ggtgaagata aaacgcctcg ctttcatcga gtacgatctc atcgctcatc
     4441 acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat aaacgctgcc
     4501 gtgtgaccac ggaaggtacg aaatttattg cccaggcgca catccaccgt cgcgccatta
     4561 atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag ccaggcttca
     4621 atatctcggt cacacagacg catggcattt tctcctttcg tgcatcactc ccttaacgcc
     4681 aattacgtca agggcatacc aggttatcac tgaacggtac acaatttatc aagcttattc
     4741 aaagaactga cttattttcg ctttcaatat atcgatggcg atgcggtttt tcccgccgcg
     4801 cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga acatcgggcg
     4861 cacggttttt tgatattgcg ccatcactga atccattgaa cgcccacgct cgttaacgtc
     4921 acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa ttgagaagtt
     4981 aagttcatca cgcaaacgcg catccgtcag cagcaaaatg ccttcgagaa tgatgacttt
     5041 cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg
     5101 caggtcaatg gccgagccgc gtttcaatgc ttgtaaatgc tcaagcagca ggctgtgatc
     5161 catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca gatggctttg
     5221 atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga cttgctcacg
     5281 taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg cgccagcgat
     5341 accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct gattaacctg
     5401 gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt tcatccgcct
     5461 gataccagtc gaatagcgtt gccgcgcgct cagagtaatt gttgacgaat aattcccggt
     5521 ggcaaattac gttgttcagt tttaatgcaa ggtaaaaatt gttatacgaa gttgcgcaaa
     5581 ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact
     5641 agcataaaca cagaaacgaa tactggcgac ctggtcttgc ggataaagcg gtaatgagca
     5701 aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg gtcagtttag
     5761 gtttagtctc gtttatcttt acccttttct cgcttgagct ttcgcagttt ggcacccaac
     5821 tcgccccatt gtggttcccg acgtccatca tgatggtggc gttttatcgc catgccgggc
     5881 gcatgtggcc ggggattgcg ctgagctgct cgctgggaaa tatcgccgca tccatcctgc
     5941 ttttttccac cagctcgctg aacatgacct ggacgaccat caatattgtt gaagccgtgg
     6001 tcggggcagt gctactgcgt aaattgctgc cgtggtataa cccattgcaa aatctggctg
     6061 actggctgcg tctggcactc ggcagcgcca ttgttccgcc tctgttgggg ggtgttctgg
     6121 ttgtcctgct gacgcccgga gacgatcctc tcagggcatt tttgatatgg gtactttcag
     6181 aatccatcgg cgcactggca ctggtgccgc tggggttgtt atttaaacca cactatctgc
     6241 tgcgccatcg caacccacgg ttgctttttg agtcgctgct cacgttagcc atcacactga
     6301 cgttaagctg gctttcgatg ctgtatctgc cgtggccttt tactttcatt attgtgctgt
     6361 tgatgtggag cgccgtgcgc ctgccacgaa tggaagcctt tttgatcttc cttaccacgg
     6421 tgatgatggt gtcactgatg atggccgctg atccctccct gcttgctacg ccgcgtacat
     6481 acctgatgag ccatatgccg tggctaccgt ttttgctgat cctgctgccc gccaacatca
     6541 tgacgatggt gatgtatgcc tttcgtgcgg aacgcaaaca catctctgaa agcgaaaccc
     6601 gctttcgtaa cgccatggaa tattccgcca tcggcatggc attagtgggc accgagggac
     6661 aatggctgca atccaacaaa gcgctctgcc agtttctcgg ttacagtcag gaagaactgc
     6721 gcggactcac ctttcagcag ctgacctggc cggaggatct caataaagat ctgcaacagg
     6781 ttgaaaagct gatcagcggt gaaataaaca cctattcaat ggaaaaacgc tactacaacc
     6841 gcaatggcga tgttgtctgg gcgttgcttg ccgtctcact ggtacgccac acggatggca
     6901 cgccgctcta ttttatcgct cagattgaag acattaacga gctaaaacgc accgaacagg
     6961 tgaatcagca actgatggag cgcatcacgc tggctaacga agcgggcggg attggcatct
     7021 gggagtggga gctaaagccg aatattttta gctgggataa gcggatgttc gagctgtatg
     7081 aaattcctcc gcatatcaaa ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag
     7141 atcgccagca cgccgaaaaa gtgattcgcg attcattgca atcacgctct ccctttaaac
     7201 tggaatttcg cattaccgtg aaagacggca ttcgccatat ccgcgccctc gctaaccggg
     7261 tactgaataa agaaggcgaa gtcgaacgcc tgctcggcat taatatggat atgaccgaag
     7321 tgaaacagct taacgaggca ttgtttcagg aaaaagagcg cctgcacatt acgctggact
     7381 ccatcggtga agccgtggtc tgtattgata tggcgatgaa aattaccttt atgaatccgg
     7441 tggcggagaa gatgagcggc tggacgcagg aagaagcatt aggtgttccg ctcctgacgg
     7501 tgttgcatat tacttttggc gacaacggac cattaatgga gaacatttac agtgccgaca
     7561 cctcacgttc cgcgattgaa caagatgtgg tgttgcactg tcggagcggc ggcagctacg
     7621 acgtgcatta tagtattacg ccgttaagta ctctggacgg cagcaatata ggctcggttc
     7681 tggtgattca ggacgtcacc gaatcacgca aaatgctgcg ccagctgagc tacagcgcct
     7741 cccatgatgc actgacgcat ctcgccaatc gcgccagttt tgagaagcaa ctgcgtatcc
     7801 tgctgcaaac ggtaaacaat acacatcagc gacatgcact ggtgtttatc gatcttgatc
     7861 gctttaaagc ggtgaatgac agcgccgggc acgccgctgg cgacgctttg ttgcgcgaac
     7921 tggcgtcatt gatgctgagt atgctgcgct ccagcgacgt gctggcgcga ctcggtggcg
     7981 atgaatttgg tctgctgctg ccagactgta atgtcgaaag tgcgcgtttt atcgctacac
     8041 gcattatcag cgccgttaat gactatcact ttatctggga aggacgagta catcgggtag
     8101 gtgccagtgc cgggattacc ttgattgatg acaacaatca tcaggcagct gaagtgatgt
     8161 cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg acggggccgg gtgacgattt
     8221 acgaaccgca gcaagctgcc acacatagcg aacgggcggc gatatcgctt gatgaacagt
     8281 ggcggatgat taaagaaaat cagttgatga tgatcgccca cggtgtcgct tcgccgcgga
     8341 tcccggaagc acgtaatttg tggctgattt cacttaagct ctggagttgc gaaggcgaga
     8401 ttattgatga acaaacattt cgtcgtagct tcagcgatcc ggcacttagc catgctcttg
     8461 accgacgggt attccacgat tttttccagc aggccgcaaa agctattgcc agtaaaggct
     8521 taagtatcgc cctccccctt tccgttgccg gtttgagtag cgccacgctg gtgaatgaac
     8581 taattgagca gctggaaaat agccctctac caccacggtt attacatctg attattccgg
     8641 cagacgcgat tttagatcac gcagaaagcg tgcaaaaact gcggctggcg ggatgtcgga
     8701 tcgtattcag tcaggtgggc cgcgatctgc aaatcttcaa ctcgctgaaa gcaaatatgg
     8761 cagattacct gctacttgat ggtgagttat gcgccaatgt gcagggaaat ttgatggatg
     8821 agatgctgat tacgatcatt caggggcacg ctcagcggct cgggatgaag accatcgccg
     8881 ggccagtagt tttacccctg gtgatggata cgctttctgg catcggcgtc gatctgattt
     8941 atggcgatgt gattgccgat gcccaaccgc tggatttgct ggtgaatagc agttatttcg
     9001 cgattaacta aggcgtgctt ccccatcgcc tgatgcggcg tatcaggcct acaattcgtc
     9061 cgttccgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca
     9121 gggcttccag cgttcggcat aacggcggat ttgtgccggt gtcattcccg gaaatcgctg
     9181 tttaatcaga taatcatccg gcaggaaaac atctttcgcc tgccagccac gtaaggcgaa
     9241 ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcattttca tcgcctgctc
     9301 cacatcgccc ggaattgtca ttgataaggt gccctccagc gccgcatttg ccagatgaat
     9361 cagtgcctct gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtcggctgc
     9421 tgccagccgc tgaggcgtgg ggaagcagac ataatccgga aaatcatcca gccgttcgcc
     9481 ataaagctgt gccactctgg cggtcaattt tgccgccatc gccacgctca ccagttggcc
     9541 taaaatcgcc cgcacgccct gctcaaaagc atcaacacaa ccaggtaaac gcaatccggg
     9601 ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc
     9661 aaacaggcgg ctcattttcg ccagacactc tgcggcaaca ggttctaaac ctgcacttaa
     9721 atttatgtgc agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc
     9781 gcccaccgcc agactacggg cataataatc gtcagcgacc gtttccacac cgttcaccgc
     9841 acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc agttcagggt
     9901 atacatcgca tctccttgtt aatccgcttt cagcataaac gttattcaga cgctacgctt
     9961 tgctttcata ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca
    10021 ccggcatggg gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt
    10081 ggcggtcatg cgcgacggta agccgcaatt actgaaaatg gaaaacgaca gcacgctgct
    10141 gccttcaatg ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca
    10201 tgatgttccg gcagacgacg atgaaacgca ggcgctgctg cgtcgggcga ttcgttataa
    10261 ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc
    10321 acagtacatt gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg
    10381 tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat
    10441 gttgcacatt cgccagcagg cgcaggcaca gctgccagaa gcgattactc aggcggtgat
    10501 tggtcgccct atcaacttcc aggggctggg cggcgatgaa gcaaacaccc aggcgcaggg
    10561 gattctggaa cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc
    10621 ggtcgcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt
    10681 ggatatcggc ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc
    10741 gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt tgccgtattg gcggtaacga
    10801 tctggatatc gcactggcgt ttaaaaacct gatgccgtta ctgggcatgg gtggcgaaac
    10861 cgaaaaaggc atcgccctgc cgattctgcc gtggtggaat gcggttgcca tcaacgacgt
    10921 acctgcgcaa agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg
    10981 cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag
    11041 ctatcgcctg gtacgtagcg cagaagagag caaaattgcg ctttcaagcg tagcggaaac
    11101 ccgcgcctca ctgccgttta tcagcgatga actggctacg ctgattagcc agcaagggct
    11161 ggaaagcgcc ctcaaccagc cactggcgcg gattctggaa caggtgcaac tggtgctgga
    11221 taacgcccag gaaaaaccgg atgttatcta tctgaccggc ggtagcgccc gttcaccgct
    11281 gattaaaaaa gcgctgacag aacagttgcc gggcattccg attgcaggtg gcgatgactt
    11341 tggctcagtc accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aattgaaaac
    11401 tggctcagca acagcttaaa tcagcctgcg cctgagccag tttttctgcc gcctgacgta
    11461 acggttccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg
    11521 tcgcaccgac agtcagcgcc gattccagct gatgtcgttt ggcggtcatt ttcgccatta
    11581 ccgcttgtct gtctactggc gttaccgctt cattcggcct gaacacaaaa cggcgttcgc
    11641 aacttgcttt ccagtcgata actgcctggg tcagatgatc accaaaacct ctaacttgct
    11701 taacgctccg tcgggtaacg tctgccgccg tttcaatacc aaaggaccgt aacgccactt
    11761 tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc tccagaaact
    11821 tctgcttctg ccgttccctt gcggtgtcct gaagtgctgc cagatcgcgc ttttcttctt
    11881 ccggtaaccc aagaatttcg tccttcattt tttcgagcat cgtccgtttg gctataaaac
    11941 cttccagccc gcccaactgt tggatctggc tgactaaatg gtcatagtcc attttcgcgc
    12001 gattaaacgc ctcacggcgt tgctggattt ctgctttgta cgctttgctt gtcaggctgc
    12061 caataatcca tatagccgac agcacaggaa ccaaaataat ataacgcggt tctgtctgaa
    12121 ggccgcaaag caacagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcac
    12181 gccttaaaag gcctaaagga agcggcctgc cagtcggttg aaaatgatcg ggtaatggca
    12241 gttgcaatgc tggtggtgct actgacgcca tcaccatcgc ccagactttc gccagcacaa
    12301 aatcaccgct ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat
    12361 tatccagcgt acaccacggg cagtcagtca aatgaccggg gtaaacatgc attgccgaaa
    12421 cggtacattt ctttaactgt tggcgtagta aatcaagtgc cgctacccac gcttttgccg
    12481 tcggacgccc ggttgccatg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat
    12541 cacccggtaa catcgacagc ggaatagatc gtggcggcgg ttttaaacca cgtcgctgat
    12601 tatctgacgc gtaggtataa cggaaatggg caatatccgt ctccagcgca ttacccgcat
    12661 cagagataag cggtacaccg gaataaggat gccgaccacc gaacaagacg tgaaaaatca
    12721 gcaacgcaag gccaaaatta tcgtgattcg cggtgcgttc aaagccgaca aatgatggca
    12781 aggtttgcag ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtgc
    12841 cattggcgtt aatctgaaag gagtcgctat caatcaacac cactttgctg tcacggccta
    12901 ccataaagct gttctggttt acgtcaccca cgacgtgccc gtgctcgtga atcgtagcaa
    12961 aagatgaagc aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgagggtaac
    13021 tctggcgacg atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg
    13081 gcatcataaa accgataact tttccgcctc gtccaccgtg aagtgttgac tgcggccagg
    13141 cgacataatt caacaactgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt
    13201 ttaaggcggg tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcctcgatat
    13261 cataaaccgc accttcaccg cctttgccca gttcacggcc tggcgtaacg cactcaccag
    13321 tagcagtata taaagtgggt ttcatggtaa ccacagcgcc agtgccaacg ttttatcatc
    13381 atcggtacgc tcgtttaccg ccgggctgga caaaaactgc tttaacaatt caggtaataa
    13441 atcgagttgt tcctgcgttg ctgacgccag tccattgaaa aacggggtaa aaaaaggcac
    13501 atgcggagaa ttattcaaca tatttaacgc cagccgctga ataccatccg taaatgctgc
    13561 aactttatgc gcacgctcag tactggtgta agtttccagt ctggaaacag catcgtcatc
    13621 ggtaatgaag tgcgtcatgt tggcatattc acccaccatc ggcgtgagag gcaattgcag
    13681 accgtgaccc aaatctacca ccacgccgcc atcgccaatc tgcataatca aggtgccatt
    13741 agctgatgaa atcaatccca aaaacgtgca ggcaaaatcg cgaaccgcta attctttggc
    13801 ttccgcctcg gcaaaaagtc gctgacgaat ggtcagcaca atgtccgttg cgaggacatc
    13861 attaagcccc aattccccgc cctgcacttt ttgcgacata taagccatcg cttcattgac
    13921 ggcgagcatc gcaccttcgc caccctgcga gacgctgcct gcgccatcag cgacaaatac
    13981 cgacaataat ggctgctggt catttaacca ggcaacctgc atctggcagg catcctggca
    14041 aggcaagtcc gcgctgatgt gcgacgttcc aacggttgat gcatagacca gacgccagct
    14101 cacactgacg tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg
    14161 gaaaccgaac gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccctgc
    14221 agtggcaaag gctgacaaac gctgatttgt gccaaagtct tcatatccgc cccctgaaca
    14281 ccgatggaaa agaaggcaaa cttcttatct tcttccccct gaaacacttt gttggcggca
    14341 gcctgccact catcggttgg agcgccatcg gtgatcagga aaatccacgg gcgataatag
    14401 gaaataccat tggcgcgata ctcacgtttg cgctcctcaa ccatatccag ggctttggta
    14461 atggcggccc ccattggtgt atcgccctgg gcaaacagga tgggcgggaa aaaattagcg
    14521 gcgctggtaa agggttgttc cacatgcacc gggccgaacg tcacaatacc gagttccact
    14581 ctttttaagg ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggcgtta
    14641 agttcattaa tgggtctacc gttcatagaa ccagaaacat ccagcagcaa aatgcagggg
    14701 caacgtggtt ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata
    14761 agaaatcctt tcttagagaa ttattgaggt ccggtatcat accgactgca acaatgaaat
    14821 atgagcaggc aaagcaactt tcagaattag gcccggcggc gcagaaaatt ttgctattcg
    14881 cctcgcaaga acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag
    14941 tgagggttag ggagaggttt ccccctcccc ctggtgttct tagtaagcct ggaagctaat
    15001 cactaagagt atcaccagta tgatgacgtg cttcatcata accctttcct tattaaaagc
    15061 cctcttctcc gggagagggt ttcccgtttc agcgtcccgc tgaaatcgtc ggcttacctc
    15121 ctttcgccct gaatgcagtc tatcgctaac gcgtgaacaa aatagtttcc tctgtgatta
    15181 ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg cagactgcaa agcctgcaga
    15241 ttgacaatct tatcgtgaag gcataatttc aggagtgagg gtagagcggg gtttcccccg
    15301 ccctggtagt cttagtaagc ggggaagctt atgactaaga gcaccacgat gatgagtagc
    15361 ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt cccgtttcag
    15421 cgtcccgctg aaatcgtcga cttacctcct ttcgccctgt atgcagtcta tcgctgactt
    15481 gcggataaaa tagtttccag tgtgattatt gcgtaacgtc tcgcaaagaa tatttgctgg
    15541 caggatcgca gactgcaaag cctgcagatt gacaatctta tcgtgaaggc atactttcag
    15601 gagtgagggt agagcggggt ttcccccgcc ttggtagtct tagtatgctg ggtagctaat
    15661 cactaagagc accacgatga tgatccactt catcatgacc ctttccttat ttaaggcccc
    15721 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcatcggct tacctccttt
    15781 cgccatgcac gtagtctatc gctaacgcgt agataaaata gtttcatgtg ttattactgg
    15841 atgcgtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt cataattcct
    15901 ccatttttct cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag
    15961 agaaactctt aacgatgaaa ggtagttata aatcccgttg ggtaatcgta atcgtggtgg
    16021 ttatcgccgc catcgctgca ttctggttct ggcaaggccg caatgactcc cagagtgcag
    16081 ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt
    16141 ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc
    16201 tcaccgggct tggcaccatt accgccgcta atactgttac ggtgcgcagc cgcgtggacg
    16261 gtcaactgat ggcgttgcat ttccaggaag gccagcaggt caaagcaggc gatttattgg
    16321 cagaaattga ccccagccag ttcaaagttg cattagcaca agcccagggc caactggcaa
    16381 aagataaagc cacgcttgcc aacgcccgcc gtgacctggc gcgttatcaa caactggtaa
    16441 aaaccaatct cgtatctcgt caggaactgg atgcccaaca agcgctggtc agtgaaaccg
    16501 aaggcaccat taaggctgat gaagcaagcg tcgccagcgc acagctgcaa ctcgactgga
    16561 gccgcatcac cgcgccagtt gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc
    16621 aaatctccag tggtgatacc accgggattg tggtgatcac ccagacgcat cctatcgatt
    16681 tgctctttac cctgccggaa agcgatatcg ctaccgtagt acaggcgcaa aaaaccggaa
    16741 aaccgctggt ggtagaagcc tgggatcgca ccaactcgaa gaaattaagt gaaggcacgc
    16801 tgttaagcct cgataaccaa atcgatgcca ctaccggtac gattaaagtg aaagcacgct
    16861 ttaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgt atgttagtcg
    16921 acaccgaaca aaacgccgtg gtgatcccca ccgccgctct gcaaatgggc aacgaaggcc
    16981 attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg acgccgggca
    17041 ttcaggacag tcagaaagtg gtgatccgcg caggtatttc tgcgggcgac cgcgtagtga
    17101 cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtagtggaa gcccagagcg
    17161 ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct
    17221 gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc
    17281 tgtcgccacc acgctgctga tggtggcgat cttactcgct gggattatcg gttatagagc
    17341 cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtag tcacgctcta
    17401 cccaggtgcg agcccggatg tcatgacctc tgccgttacc gcgccgcttg aacgccagtt
    17461 tgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat
    17521 caccttacag ttccagctaa cattaccgct ggatgtcgcc gagcaggaag tgcaggccgc
    17581 gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa
    17641 agtgaacccg gcagatccac caatcatgac gctcgccgtc acctcaaccg ccatgccaat
    17701 gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg
    17761 tgtcggcctg gtgacgcttt ccggcggcca gcgtccggct gttcgtgtca aacttaacgc
    17821 tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcta ttaccggcgc
    17881 taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgtgcggtca cgctttccgc
    17941 gaacgaccag atgcaatccg ccgaagagta tcgccagctg atcatcgcct accagaacgg
    18001 cgcgccgatt cgtcttggcg atgtcgcaac cgtagaacaa ggcgcagaaa acagctggct
    18061 cggcgcgtgg gcgaacaaag aacaggccat cgtgatgaat gttcagcgcc agcccggtgc
    18121 taacattatc tccaccgccg acagcatccg gcagatgctg ccacaactca ccgagagttt
    18181 gccgaaatcg gtaaaagtga cggtactttc tgaccgtacc accaatattc gcgcatccgt
    18241 cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct
    18301 gtttttgcgt aatattccgg cgactatcat tcccggcgtc gcagtgccgc tgtcgttaat
    18361 cggcaccttc gcggtcatgg tgtttctcga tttttcaatc aataacctga cgctgatggc
    18421 gttaactatc gccaccggat ttgtggtcga tgacgccatc gtggtgatcg aaaacatttc
    18481 ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaaaggcg cgggtgaaat
    18541 cggctttacc attatctcgc tgaccttctc actgattgca gtgttgatcc cactgctatt
    18601 tatgggcgat atcgtcgggc gactgttccg cgaatttgct attactctgg cagtcgcgat
    18661 tttgatctca gccgtggtgt cgctgactct gacgccgatg atgtgcgcgc ggatgctcag
    18721 ccaggagtcg ctgcgtaaac agaatcgctt ctcccgagcc tcggagaaaa tgttcgacag
    18781 gataatcgcc gcctatggtc gtggactggc gaaagtgctg aatcatccgt ggctgacctt
    18841 aagcgtggca ctcagcacgc tgctgcttag tgttctcttg tgggtattca ttccgaaagg
    18901 tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac cgcaatccag
    18961 ctcctttgcc aatatggccc agcggcaacg ccaggtcgcg gatgtgattt tgcaggatcc
    19021 ggcagtgcaa agcctgacct catttgttgg cgttgatggc actaacccgt cgctgaacag
    19081 tgcgcgtttg cagatcaacc tcaaaccact ggatgaacga gatgaccggg tgcaaaaagt
    19141 catcgcccgt ctgcaaacgg cggtggataa agtgccgggc gtcgatctct tcctgcaacc
    19201 aacgcaggac ctgaccatcg atactcaggt cagccgcacc cagtaccagt ttaccttgca
    19261 ggccacgtca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca
    19321 gctgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt
    19381 caatgtcgac agggacagcg ccagccgtct ggggatcagt atggcggatg tcgataacgc
    19441 cctgtacaac gcgtttggtc agcggctgat ttccaccatt tatactcagg ccaaccagta
    19501 tcgcgtggtg ctggagcaca ataccgaaaa taccccaggg cttgcggcgc tggataccat
    19561 tcgcctgacc agcagcgacg gtggcgtggt gccgttaagc tcaattgcca aaattgagca
    19621 gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt
    19681 taacgtaccg gataactatt cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa
    19741 gacgctgaat ctgccggtgg atatcaccac gcagttccag ggcagcaccc tcgccttcca
    19801 gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt
    19861 gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac
    19921 cgcaggggtc ggcgcactgc tggcgttaat gattgccggt agcgaactgg atgtgattgc
    19981 cattatcggc attattttgc tgatcggtat cgtgaagaag aacgccatca tgatgatcga
    20041 cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcca tctaccaggc
    20101 ttgtctgttg cgttttcgtc cgatcctgat gaccactctg gcggctctgc ttggcgcgct
    20161 gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat
    20221 ggtcggcggt ctgattgtca gccaggtgct gacgctgttt accacgccag tgatttatct
    20281 gctattcgac cgcctggcat tgtggaccaa aagccgcttt gcccgtcatg aagaggaggc
    20341 gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt tactgtcggt
    20401 cgccattacc ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc cgctgccgca
    20461 ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac cagaaacaat
    20521 ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg cgcattgccg gggtcagtga
    20581 aatgacctcc agcagttcgc tcggcagcac gcgcattatt ttgcagtttg attttgaccg
    20641 cgatatcaac ggcgcagcgc gcgatgtgca agcggcgatc aacgccgcgc aaagtttgct
    20701 gcccagcggg atgccaagcc gtccgaccta tcgcaaagcg aacccgtcgg atgcgccaat
    20761 aatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg atttcgcctc
    20821 gacgcagctg gctccgacga tttcgcaaat cgacggtgtt ggtgatgtcg atgtcggcgg
    20881 cagctcactg cccgccgtac gcgtcgggct gaatccgcag gcgctgttta atcagggcgt
    20941 gtcgctggac gatgtgcgca ccgccatcag caatgccaac gtgcgtaaac cgcagggggc
    21001 gctggaagat gatactcacc gctggcagat ccagactaac gatgaactga aaaccgccgc
    21061 tgaatatcag ccgttgatta ttcattacaa caacggcggc gcggttcgtc tgggcgatgt
    21121 ggcgacggtg accgactctg tacaagatgt acgtaacgcc gggatgacca acgccaaacc
    21181 ggctattttg ctgatgatcc gcaagctgcc ggaagccaat ataatccaga cggttgacag
    21241 catccgggca aaattaccgg agttgcagga gaccattccg gcggcgattg atctgcaaat
    21301 tgcccaggat cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat
    21361 tatctcggtg gcgctggtga ttctggtggt gtttttattc ctgcgctcgg gtcgcgccac
    21421 tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct
    21481 gtgcggattc agtctcaata acctgtcgtt aatggcgctc actattgcta ctggtttcgt
    21541 ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt cacctggaag cggggataaa
    21601 accactacag gccgcactgc aaggtactcg cgaagtcggt tttacggtgc tgtcgatgag
    21661 tctttcactg gtggcggtgt ttctgccgct gctgttgatg ggcggattgc cgggccgact
    21721 gttacgcgag tttgccgtga cgctttctgt cgccattggt atttcgttgc tggtttccct
    21781 gacgctaacg ccgatgatgt gtggctggat gctgaaagcc agcaagccgc gcgagcaaaa
    21841 gcgactgcgt ggttttggtc gcatgttggt agccctgcaa caaggctatg gcaagtcgct
    21901 gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg ctgcttggca ccattgcgct
    21961 gaatatctgg ttatatatct cgatcccgaa aaccttcttc ccggagcagg acaccggcgt
    22021 gttgataggc gggatacagg cggatcagag tatttcgttt caggcgatgc gcggtaagtt
    22081 gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggatttac
    22141 cggcggttcg cgagtgaaca gcgggatgat gtttatcacc ctcaagccac gcgacgaacg
    22201 cagcgaaaca gcgcagcaaa ttatcgaccg tctgcgcgtt aagctggcga aagaaccggg
    22261 ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc agtcgaacgc
    22321 cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat gggagccgaa
    22381 aatccgcaaa aaactggcga cattgccgga actggctgac gtgaactccg atcagcagga
    22441 taacggcgcg gagatgaatc tggtttacga tcgcgacacc atggcacggc tgggaatcga
    22501 cgtacaggcc gccaacagcc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat
    22561 ttaccagccg atgaaccagt ataaagtggt aatggaagtg gatccgcgct atacccagga
    22621 catcagcgcg ctggaaaaaa tgttcgttat caataacgaa ggaaaagcga tcccgctgtc
    22681 gtatttcgct aaatggcaac cggcgaatgc tccgctgtcg gtgaatcatc agggattatc
    22741 ggcagcctcg accatctcgt tcaacctgcc gaccgggaaa tcgctctcgg acgccagtgc
    22801 agcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg gtagttttgc
    22861 cggcacggcg caggtgttcc aggagacgat gaactcgcag gtgatcctga ttatcgccgc
    22921 catcgccacg gtgtatatcg tgctgggtat cctttacgag agttacgtac atccgctgac
    22981 gattctctcc accctgccct cggcgggcgt tggagcgctg ctggcgctgg aactgttcaa
    23041 tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca tcgtaaagaa
    23101 aaacgccatc atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc
    23161 gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga tgactaccct
    23221 ggcggcgctg tttggcgcac tgccgctggt gttgtcgggc ggtgacggct cggagctgcg
    23281 ccaacccctg gggatcacca ttgtcggcgg tctggtaatg agccagcttc tgacgctgta
    23341 taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaacc
    23401 taaacaaacg gtaaccgagt aaatgacaga tcttcccgac agcacccgct ggcaattgtg
    23461 gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtca acaccgcgct
    23521 tccctcaatg gcacaaagcc tcggggaaag tccgttgcgt atgcacatgg tcattgtctc
    23581 ttatgtactg accgtggcgg tgatgttgcc tgccagcggc tggctggcgg acaaagtcgg
    23641 cgtgcgcaat attttcttta ccgccatcgt gctgtttacc ctcggttcac tgttttgcgc
    23701 gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggtgc
    23761 gatgatggtg ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg agcaatatat
    23821 ggcggcgatg acctttgtca cagtgcccgg tcaggtcggt ccgctgctgg gtccggcgct
    23881 cggcggtctg ctggtggagt acgcatcgtg gcactggatc tttttgatca acattccggt
    23941 ggggattatc ggtgcgatcg caacactaat gttgatgccg aactacacca tgcagacgcg
    24001 gcgctttgat ctttccggct ttttattgct ggcggttggc atggcggtat taaccctggc
    24061 gctggatggc agtaaaggta caggtttatc gccgctggcg attactggcc tggtcgctgt
    24121 tggcgtggta gcactggtgc tttatctgct gcacgccaga aataacaacc gcgctctttt
    24181 cagtctgaaa ctgttccgca ctcgcacctt ttcgctgggc ctggcgggga gctttgccgg
    24241 acgtattggc agtggtatgt tgccctttat gacgccggtt ttcctgcaaa ttggccttgg
    24301 cttctcaccg tttcatgccg gactgatgat gatcccgatg gtgcttggca gcatggggat
    24361 gaagcggatt gttgtacagg tggtgaatcg ctttggttat cgtcgggtac tgatagcgac
    24421 cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgctctgt tgggctggta
    24481 ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgaccc gtttctcctc
    24541 catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg gcaatagtct
    24601 gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc accatcgccg ggctgttgct
    24661 gggacttttt ggttcgcagc atgtcagcgt cgacagcggc accacacaaa ccgtctttat
    24721 gtacacctgg cttagcatgg cgtttatcat cgcccttccg gcgttcatct ttgccagagt
    24781 gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg cgcaatgaag
    24841 ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac ctgcattgtc
    24901 ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt tattgattac
    24961 atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg atgcgcttgg cgagcagtat
    25021 gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc gctttgtctt tcagatcttg
    25081 cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc gccgcacggc
    25141 tggcgtaccc agttctgggt ggtcgatcaa aacaacaaag tgctggttgg tccgcgagcg
    25201 ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga agtgggtgcg
    25261 gtgattgcct cccctgttga gcgactgacc cgcaatactg atatcaattt cgataaacaa
    25321 cagcggcaaa ccagctggct gattgttgct ctggcaacgt tactcgcggc actcgctacc
    25381 tttctgctgg cgcgtggttt gctggcaccg gtaaaacggc tggtggacgg cacgcacaaa
    25441 ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga actgggcaaa
    25501 ctggcgcaag acttcaacca gcttgccagc acgctggaga aaaaccagca aatgcggcgc
    25561 gattttatgg ccgatatttc tcacgaactg cgtacgccat tagcggtgct gcgcggtgaa
    25621 ctggaagcca ttcaggatgg cgtgcgtaaa ttcacgccgg agacggtggc gtctttacag
    25681 gcggaggtcg gtacactgac caaactggtt gacgatctcc atcagttgtc gatgtctgat
    25741 gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct ggaagtggcg
    25801 ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga aactgcaatt ttccctgcca
    25861 gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa taacttactg
    25921 gaaaacagcc tgcgctacac tgacagcggc ggtagcctga aaatctctgc cgggcagcac
    25981 gacaaaacgg tgcgactgac ctttgccgac agcgcgccgg gcgtcagtga cgatcagcta
    26041 caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc gcaaccgagc cagcggcggt
    26101 tccgggctgg ggctggcgat ttgcctgaac attgttgaag cacataatgg tcgcattatt
    26161 gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct ggaacgggat
    26221 ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt attttgatcg
    26281 tggaagatga accgaagctg ggacagttgc tcattgatta tctgcgtgct gcgagctatg
    26341 cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag acaccaccgg
    26401 atctgatcct gttggatctg atgctccctg gcaccgatgg cttgacgctg tgccgggaaa
    26461 ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc aaaaatagaa gagatcgacc
    26521 gcctgctcgg actggagatt ggcgcagatg attatatctg taagccgtac agcccacggg
    26581 aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa accgcagcgt gagttgcagc
    26641 aacaggatgc tgaaagcccg ttgattatcg acgaaggtcg ttttcaggct tcatggcgcg
    26701 gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct gaaaacgctc tctcatgaac
    26761 caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac taccgcgtag
    26821 taaccgaccg caccatcgac agccacatta aaaacctgcg ccgcaagctg gaatctctcg
    26881 acgccgaaca gtcctttatc cgtgccgttt acggcgtcgg ttaccgctgg gaagccgacg
    26941 cctgccgcat cgtttaacat ttttacctgc cggagaaggc attcacgccg catccggcaa
    27001 cataatccca cgcatggtca gcaaactgac attatctctc cgccctggca ggcaccagaa
    27061 aatcgctcat actttaatcg gtaaacagtc cttttagatg ctgttttcga tacgcaattt
    27121 caatcaagga gtcattatgg ctggttggtt tgaactcagt aaaagcagtg ataatcaatt
    27181 tcggtttgtg ctaaaagcgg gcaatggtga gactatcctt accagcgagc tgtatacctc
    27241 aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc aagaagaacg
    27301 ctatgagaaa aaaacggcaa gcaacggcaa attctatttc aatctgaaag ccgctaatca
    27361 tcaaattatc ggctccagcc agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc
    27421 ctccgttaaa gccaatggca caagccagac ggtgaaagac aatacgtaat tacgataccg
    27481 ggcgtgggtt gttggcgtcc ggcgctttac cagctccccc ctacagcgct acaatgcccg
    27541 cccttaaagt gggggcactc ccctggccgc ttcatcaggt gaagcggatc tgacctgtca
    27601 tcagaacgag agaattatgt ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa
    27661 tatgcgttac gctttcgctt atggcgcaga tgctgtttat gcgggccagc cgcgttactc
    27721 cctgcgtgtg cgcaacaacg aatttaacca cgaaaatctt cagctcggca tcaatgaagc
    27781 ccacgcgctg gggaaaaagt tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct
    27841 gaaaaccttt atccgtgacc tgaaaccggt ggtggaaatg gggccggatg cgctgattat
    27901 gtctgatcca gggctgatta tgctggtgcg cgagcacttc cctgaaatgc cgatccatct
    27961 ctcagtacag gctaacgccg tgaactgggc gacggtgaaa ttctggcagc aaatgggtct
    28021 gacccgcgtg atcctctctc gcgaactgtc actggaagag attgaagaga tccgcaatca
    28081 ggtgccagat atggagatcg aaatcttcgt tcacggcgcg ctgtgcatgg cctactccgg
    28141 tcgctgcctg ctctctggct atatcaacaa gcgcgacccg aaccagggca cctgcaccaa
    28201 cgcctgccgc tgggagtata acgtccagga agggaaagaa gatgacgtcg gcaacatcgt
    28261 acacaagtac gaaccgattc cggtgcaaaa cgttgaaccg acgctgggga tcggcgcacc
    28321 aaccgacaaa gtgtttatga tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt
    28381 tgaagatgag cacggcactt acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt
    28441 tgaacgcctg accaaaatgg gcgtgcattc gctgaaaatc gaaggccgta ccaaatcttt
    28501 ctactattgc gcacgcaccg cgcaggttta ccgtaaagct atcgatgacg cagctgcggg
    28561 aaaaccattc gataccagcc tgctggaaac cctggaaggt ctggcgcatc gtggctatac
    28621 cgaaggtttc ctgcgtcgtc atactcacga cgattatcag aactacgaat acggttattc
    28681 agtttctgac cgccagcagt ttgttggtga gtttaccagt gagcgcaagg gcgacctcgc
    28741 ggcggtagcg gtgaaaaata aattctccgt tggcgacagc cttgagctga tgacgccgca
    28801 agggaacatt aactttaccc ttgagcacat ggaaaacgcc aaaggcgaag caatgccggt
    28861 cgcaccgggc gatggttata ctgtgtggat cccggtgccg caggatcttg agctaaatta
    28921 cgcgctgctg atgcgtaatt tctccgggga aaccacgcgt aacccccacg gtaagtgatt
    28981 aatttcgatt atttttcccg gatggaaaat tcttagaaac cgatcacata cagctgcatt
    29041 tattaaggtt atcatctgtt tcgctgaaaa acataaccca taaaatgcta gctgtaccag
    29101 gaaccacctc cttagcctgt gtaatctccc ttacacgggc ttattttttg tgcataaacc
    29161 atagagacgg attgatccca ggtgaccgtc ttccattgac cacatcgata gaatctccct
    29221 tcatagcacg atgcctttca cgtaacggca tcgtgctcgc acaggttccg gctaagcaca
    29281 accagaacgc gcatgtttga cgcttaccaa aaaatattct cactctccac atttgaatgt
    29341 cagacgagcg acgccatgta atcctgcacc ttctgtcttc aggtcaacta tctgcatttt
    29401 tttgccctga gtaacacaga aatgagctgc atcatttttt actatatttt ctgcaccaga
    29461 tattctgccc ctggctaaag aagcttcggc ttcggtgtag tattggttat cgagtttacg
    29521 ctgaatatta cttttatatg caagaccaaa tttaccgata cttgtctcat cattatgtac
    29581 agcacaccca gacataagaa aaacactaat taatgatata gcagctatct ttttcatctt
    29641 accttcc
//
LOCUS       DARNVM010000045        29029 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.045, whole genome shotgun sequence.
ACCESSION   DARNVM010000045 DARNVM010000000
VERSION     DARNVM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.045"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(267..938)
                     /gene="queE"
                     /locus_tag="SI621_001789"
     CDS             complement(267..938)
                     /gene="queE"
                     /locus_tag="SI621_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEI4064517.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1077..1218
                     /locus_tag="SI621_001790"
                     /pseudo
     CDS             1077..1218
                     /locus_tag="SI621_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1232..2104
                     /locus_tag="SI621_001791"
     CDS             1232..2104
                     /locus_tag="SI621_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEI4064518.1"
                     /translation="MRYFILIFTFVCSFVAAQPTIVPSLQQQVTDLTSSLNSQEKKEL
                     TYKLESIFNNTQVQIAVLIVPTTKDETIEKYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PANPSEIESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSSAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(2164..3462)
                     /gene="eno"
                     /locus_tag="SI621_001792"
     CDS             complement(2164..3462)
                     /gene="eno"
                     /locus_tag="SI621_001792"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEI4064519.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3550..5187)
                     /gene="pyrG"
                     /locus_tag="SI621_001793"
     CDS             complement(3550..5187)
                     /gene="pyrG"
                     /locus_tag="SI621_001793"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HEI4064520.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5415..6206)
                     /gene="mazG"
                     /locus_tag="SI621_001794"
     CDS             complement(5415..6206)
                     /gene="mazG"
                     /locus_tag="SI621_001794"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEI4064521.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6278..6613)
                     /gene="mazF"
                     /locus_tag="SI621_001795"
     CDS             complement(6278..6613)
                     /gene="mazF"
                     /locus_tag="SI621_001795"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEI4064522.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(6613..6861)
                     /gene="mazE"
                     /locus_tag="SI621_001796"
     CDS             complement(6613..6861)
                     /gene="mazE"
                     /locus_tag="SI621_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HEI4064523.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(6939..9173)
                     /gene="relA"
                     /locus_tag="SI621_001797"
     CDS             complement(6939..9173)
                     /gene="relA"
                     /locus_tag="SI621_001797"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HEI4064524.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(9221..10522)
                     /gene="rlmD"
                     /locus_tag="SI621_001798"
     CDS             complement(9221..10522)
                     /gene="rlmD"
                     /locus_tag="SI621_001798"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEI4064525.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASEDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVAGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     CALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNDLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10579..13335
                     /gene="barA"
                     /locus_tag="SI621_001799"
     CDS             10579..13335
                     /gene="barA"
                     /locus_tag="SI621_001799"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HEI4064526.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(13576..14916)
                     /gene="gudD"
                     /locus_tag="SI621_001800"
     CDS             complement(13576..14916)
                     /gene="gudD"
                     /locus_tag="SI621_001800"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEI4064527.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCVWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQCFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14937..16277)
                     /locus_tag="SI621_001801"
     CDS             complement(14937..16277)
                     /locus_tag="SI621_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEI4064528.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(16279..17631)
                     /gene="gudP"
                     /locus_tag="SI621_001802"
     CDS             complement(16279..17631)
                     /gene="gudP"
                     /locus_tag="SI621_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HEI4064529.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFFWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(18066..18515)
                     /locus_tag="SI621_001803"
     CDS             complement(18066..18515)
                     /locus_tag="SI621_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEI4064530.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            complement(18533..19315)
                     /gene="truC"
                     /locus_tag="SI621_001804"
     CDS             complement(18533..19315)
                     /gene="truC"
                     /locus_tag="SI621_001804"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HEI4064531.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(19315..19644)
                     /locus_tag="SI621_001805"
     CDS             complement(19315..19644)
                     /locus_tag="SI621_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HEI4064532.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWQNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLENNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(20266..20811)
                     /gene="syd"
                     /locus_tag="SI621_001806"
     CDS             complement(20266..20811)
                     /gene="syd"
                     /locus_tag="SI621_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HEI4064533.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRAHLASNLAEFLNQLKPLL"
     gene            20879..21727
                     /gene="queF"
                     /locus_tag="SI621_001807"
     CDS             20879..21727
                     /gene="queF"
                     /locus_tag="SI621_001807"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEI4064534.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21839..23203
                     /gene="ppnN"
                     /locus_tag="SI621_001808"
                     /gene_synonym="ygdH"
     CDS             21839..23203
                     /gene="ppnN"
                     /locus_tag="SI621_001808"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HEI4064535.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            23759..25048
                     /gene="sdaC"
                     /locus_tag="SI621_001809"
     CDS             23759..25048
                     /gene="sdaC"
                     /locus_tag="SI621_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HEI4064536.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFN"
     gene            25106..26473
                     /gene="sdaB"
                     /locus_tag="SI621_001810"
     CDS             25106..26473
                     /gene="sdaB"
                     /locus_tag="SI621_001810"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HEI4064537.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQDTGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            26584..27339
                     /gene="xni"
                     /locus_tag="SI621_001811"
     CDS             26584..27339
                     /gene="xni"
                     /locus_tag="SI621_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HEI4064538.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRNSGWRHQRLPDYKAGRPPMPEELHDEMPALRTAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(27394..28542)
                     /gene="fucO"
                     /locus_tag="SI621_001812"
     CDS             complement(27394..28542)
                     /gene="fucO"
                     /locus_tag="SI621_001812"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEI4064539.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(28570..>29029)
                     /locus_tag="SI621_001813"
     CDS             complement(28570..>29029)
                     /locus_tag="SI621_001813"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEI4064540.1"
                     /translation="HEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRP
                     IPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLE
                     KALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7082 a         7707 c         6949 g         7291 t
ORIGIN      
        1 aaacaacaca tccattatgt taaagagcac ttaattcatt gattgaaaag ccaaatattt
       61 aaagtaattc tataccgact aaaatccacc aaactccgac gtaataattt tcactccaac
      121 gaaaaattaa cgccacagaa tttgtagaaa atgataatga ttagtccagg atgggcaaaa
      181 tggaggtttt ttagaggcga caaagaatat aaatgagtag aagtctttac aggttccacc
      241 catttataac gcttataaat gtgtaatcag gcaatattta aatatttatg tgtttgcatc
      301 gacaaacgcc aattacgcgc aatgcaggtt tcaatgcaca aacgtgtggc atcatccttt
      361 tggctaatcg gctgcagtgc aatgactcgc ggtttatcat cggtcagcgt cgcaagcaat
      421 tcatccagtg cttcgatatc acgtacgcgt ccaactgggt gtttgatttc gttagctcgc
      481 tccagggctt gcgacaatac ttcataacca ccacgcatat ttagctttgg cgacacggtg
      541 acccaggtat tcggcgtaca acgcacttca tgagtaccgc tggtttctat ctggcagcta
      601 aaaccgttct tttcgagcag gtcagtcagt ggcagcaaat catgaatgca aggctcacca
      661 cccgtaatca ccacatgccg cgcggtgtat ccctggcgac caatgacagc cagcaagtct
      721 tcactgctcg ccgcccccca cttatcactc tctttggtct tcgccagaat gctgaaaagg
      781 gagacttccc gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca ggcacagcca
      841 accgggcatc cctgtaaacg aataaaaatg gcggggacgc cggtaaagta accctcacct
      901 tgcagggtct ggaacatctc gttaatcggg tactgcatag cattctctgt gaagtggata
      961 attgttaatt attgcagatc ctgccacaac aatcatgtct tattaacatt ctgttacagg
     1021 caggtttaaa gaaaaatgag aaacagatcg ttaataataa aagggagtaa cgtattatgt
     1081 cagaaataaa taaagtaaag ggatttaatc atgtcaaaaa cattcaccaa aattatactt
     1141 attttttctg tgttcgtttt taattataac gaatctcaaa ttaccgatgc cgccgtcaat
     1201 ttatttatcc agctttaatg ggacatttat aatgcgctat ttcatactaa tattcacatt
     1261 cgtgtgttcc tttgttgcag cccaaccaac tattgtccca tcattacagc aacaagttac
     1321 ggatcttacg agtagcttaa actcacaaga aaagaaagaa ctgacgtaca agttagaatc
     1381 tattttcaat aatacccaag tgcaaattgc tgtattaatt gttcccacaa ccaaagacga
     1441 gacaattgaa aaatatgcta cacgggtttt tgacaattgg cgtttaggag atgccaaacg
     1501 taatgatggg atactgatca ttgttgcctg gtcggatcgt actgtccgca tccaggtagg
     1561 ttatgggctg gaagaaaagg taaccgatgc tctggctgga gatatcatcc gtagcaacat
     1621 gatacccgca tttaaacaac aaaaattagc tcagggatta gagctagcta taaacgcttt
     1681 gaataaccaa ctcacttcac aacaccaata tccggctaat ccttcagaaa ttgaatcagc
     1741 gtcttccagt gatcattatt actttgctat tttttgggta tttgcagtga tgttcttccc
     1801 tttctggttt tttcatcaag gcagtaattt ttgtcgcgca tgtaaaagca gtgtctgtat
     1861 ttcagcaatc tatcttttag atttattcct gttctccgat aaaacttttt ccagtgctgt
     1921 atttttcttc ttttttactt tcaccaccat tatggtcttt acctgtttat gtgtacttca
     1981 gaaaaaagca tctggtagaa gttatcattc agacaacagc ggttccggtg gcggctcaag
     2041 tggcggcgga tttagcggcg ggggcggttc ttctggcggt ggcggcgcgt ccggtcgctg
     2101 gtaactcaac cataaaaaat gccagcccga aggctggcat ttttaaatca gataaagtca
     2161 gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc
     2221 ttcttcgata cgaatcagct ggttgtattt agcaacgcgg tcagaacggc tcatagaacc
     2281 agttttgatc tggcctgcag cagtaccaac agccaggtca gcgatggtag catcttcagt
     2341 ttcgccagaa cggtgagaga ttactgcagt gtagccagcg tctttcgcca tcttgattgc
     2401 agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat
     2461 acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac
     2521 cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc
     2581 gtccagaccg tcttcgatgg atacgatcgg atactgtttg gtcagttctt ccaggaagtg
     2641 agtgaattct tcagaggtga atgctttgtt gccttcgcca gccagaacgt atttaccgtc
     2701 tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata
     2761 gccagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt
     2821 cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt tcagaacttt
     2881 tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc
     2941 aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt
     3001 gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc
     3061 gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga
     3121 tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat
     3181 catgatcttg tcaatgccag cctgatcttt ggcatctttg ccaatcagtg cctgagcgat
     3241 cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc
     3301 gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc
     3361 cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga
     3421 gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca
     3481 ctagttaaac tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa
     3541 cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc
     3601 aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac
     3661 gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc
     3721 cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg
     3781 gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc
     3841 aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct
     3901 acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta
     3961 cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa catggcgagc
     4021 gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc
     4081 acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg
     4141 tacgaggatt gcgtccagac ctttaagaat ttcaacgccg cgcgtttcaa catcttgtga
     4201 atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc
     4261 gatcacggat ttgtaagcgt ccggcagttc aatgtacttg ccgaccatac caatggtgac
     4321 ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca gattcgcttc
     4381 cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgcg atttcaacag
     4441 gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac
     4501 attacagaac aatgcaatct tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca
     4561 aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg
     4621 tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag
     4681 ggtgtgctca cggccaattt caacagccat ctggcgaatc gcttcgagga acggcaggga
     4741 ttcgatatca cctactgtac cgccgatttc gaccagtact acgtcatgac cttcgccgcc
     4801 ttccagcacg cgctctttga tagcgttagt gatgtgcgga ataacctgta cggttgcgcc
     4861 gaggtagtcg ccacggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa
     4921 gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca ggtccaggtc
     4981 ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc
     5041 tggatcgacg ttgatgtacg gatccagttt catgatggtc acattgaggc cacgggcttc
     5101 aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc
     5161 ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat
     5221 ggaataacca ggacgggaaa gcagtatacc cgaacatgac cagtgccaca aactttcatt
     5281 atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg
     5341 taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atcgcgcttg
     5401 accacgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc
     5461 attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact
     5521 tcgcgaaaac gacgctcgaa tttttcgttc gctttttgca atgcgatttc cgcttttgtc
     5581 cctaaatgac gagccagatt gaccgtggca aacagcaggt cgcccatttc ctcctccagt
     5641 ttagcctggt cgataacagc ctgccgtgcc tcgtacatca cctcgtcgat ctcttcgtag
     5701 actttatcga ctaccggacc aagcgtcgtc cagtcaaaac caacgttggc gcaacgtttc
     5761 tggatttttt gcgcacgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa
     5821 tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca
     5881 ctactgtttt cggcagaact gtcagcaaaa acatgcggat ggcgacgttc taatttatcg
     5941 ctgatcgcgg cgcatatatc attaaagtca aagcgccctt cttcctgagc catttgtgcg
     6001 taaaacacca cctggaacag cagatcgccc agttcaccgc gaagatcgtc aaaatcttca
     6061 cgggcgatgg cgtccagcac ctcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca
     6121 aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg ctgcatgatg
     6181 gtgagcaaac ggtcgatttg attcattaga atgtcctgaa aattgcgggt ccgtcaggtg
     6241 gaaacctgtg accagaatag aagtgagtta gtaacaccta cccaatcagc acgttaattt
     6301 tggctttaat gagctgtaat tcctctgggg caaccgttcc tttcttcgtt gctcctcttg
     6361 cccgccaggc gatacttttt acctgatcag ctaacgctac gccatcacgt tcctgaccgg
     6421 ataaaacaac ttcgaacgga tatccttttg attgcgttgt acaaggaaca cacagacaca
     6481 tacctgtttt gttgttgtac atgaacggac tcaggacaac agccggacga tgtccggctt
     6541 gctcgctacc ttttgtcggg tcaaaatcaa cccaaatcag atcgcccata tcgggtacgt
     6601 atcggcttac cattaccaga cttccttatc tttcggctct ccccagtcga tattctcgtg
     6661 gaggttttcc ggcgtgatgt cgttgaccag ttcagcaagc gtaaatacgg gctctttacg
     6721 cactggctca ataattaatt tgccatccac caggtcaatc ttcacttcat catcaatatt
     6781 cagattgagc gcctgcatta acgtagccgg gatccgcacc gccggtgaat ttccccaacg
     6841 ctttacgcta ctgtggatca taaccctttc ctcaaaccgc tatcatatgt agatacagta
     6901 tatatcaatc tacattgtag atacgagcta atttcggcct aactcccgtg caaccgacgc
     6961 gcgtcgataa catccggcac ctggttgagt ttacccagca cgcgacccag cacctgcagg
     7021 ttgtaaatct caatggtcat gtcgatggtc gccagttgct gtttggtgtc gctacggctg
     7081 gcaacgccaa gcacgttcac cttctcgttg gcgagaatgg tcgtgatatc acgtaacaac
     7141 ccactacgat cattagccac cacgcggacc actagcgaat atccggcgga gtagctctcg
     7201 ccccataccg cgtcaacaat gcgttctggc gcatgggagc gcagttccgc cagttgctca
     7261 cagtctgcac gatgcactga aataccgcgt ccctgggtaa tgaagccgac aatctcatct
     7321 ccaggaatcg gctggcagca gcgcgcgatg tggtgcatca ggttaccgac accttcaacc
     7381 accacacgac cgttatcttt actgcggttt tgcggcgtat agcttttttg ctgaagttgc
     7441 ttcagcgcgg cggcatcctg ctcttcggca ctcggcttat taaattgcga ttgcaggaag
     7501 ttcaccatct gattgagacg gatatccccg ccaccaattg ccgccagcaa ctcgtcgaca
     7561 tcattgaagt tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct gatccccaga
     7621 tgttccagct cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc acggtcctgt
     7681 ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta accgaggttt
     7741 gggtttaacc agtcacggct ggggttcggc tgtttctggg tgataatttc aatctggtcg
     7801 cccatctgca gctggtaggt gaacggcaca atgcgcccgc taatttttgc cccgatgcag
     7861 cggtgcccga catcactgtg gatgtggtaa gcgaagtcca gcggcgttga tcccgcaggc
     7921 aaatcaacga cgtcaccttt cggcgtaaag acgtacaccc ggtcgtcaaa gacctggctg
     7981 cgtacttcgt cgagcatttc gccggaatca gccatctctt cctgccacgc aatcagttta
     8041 cgcagccagg caatccggtc ttcatgtccc gaacgtgcgc cgccagctgc cgcgccctct
     8101 ttatatttcc agtgcgcagc aacacccaac tctgcatctt catgcatctg tttagtgcgg
     8161 atttggatct caacggtttt gccacctggt cccagaacca cggtatgaat agactgataa
     8221 ccgtttggtt tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg gcgatagtga
     8281 gtgtgcacta tccccagtgc ggcatagcaa tcctgtaaac gctcggcgac aatacgtacc
     8341 gcacgcacat caaacagctc atcgaaggcg aggttctttt tctgcatttt gcgccagatg
     8401 ctgtagatgt gtttcggtcg accatacact tcagctttaa cgccttcagc tttcatctca
     8461 gcgcgcagat ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg gcgttcatgc
     8521 agcagttttg cgatgcgttt gtattcggtc gggtggagat aacggaagca gtaatcttcc
     8581 agctcccatt tcagttgtcc aatcccaaga cggttcgcca acggcgcgta gatattggtg
     8641 cactcttttg ccgccagtac acgttcatct tccggcgcat cttttacttc gcgcagatga
     8701 gcaatacgct ctgccagttt gatgaccacg cagcgaaaat catcgaccat cgccaataac
     8761 atccggcgaa cgttatcgac ctgttcggag gaaacagaat cggtatgcgt cgctttcagc
     8821 tggcggatcg ccgccatatc acgcacgccg tgaataaggt taacgaccga cttaccgacg
     8881 ctctcacgca gcacatcttc gctgactacg ttggcatcgg ccagagggaa caacagcgcc
     8941 gcccgcagcg tgtcaatgtc catacttaat gtcgagagga tctccaccat ctcaacacca
     9001 cgccacaata acagactggc atccggatgc ccctgcgtct gttgcagaca atacgcccag
     9061 gtttcggcta agcactcaca cgacttctgg ctggtaatac ccagacttgc gatccatttt
     9121 tccggatcaa attcaccagc cttattgata tgtgcacttc ttaccgcaac catcgtcctc
     9181 tcctttaggg accagacctg ccgaaatcgg caaatcgtaa ctatttaacg cgcgagaaaa
     9241 gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc agtcgcgcaa
     9301 tggtatatcc tgcttttaat aacgcttcgc tatcccgagc cagcgttgca gggttacagg
     9361 atacataaac tatacgaata ggttccagtt ttataatttg ctgcataaca cctgcggcac
     9421 ctgctcgcgc ggggtccagc aacactttat cgaagccgtt tttcgcccac ggctgctttg
     9481 tgacatcttc ttcaagattt tcgtgataaa acgtcacatt gtgtaagtca ttaagacgcg
     9541 cattctgctg gcctttttcc accagcgccg gaacaccttc tacacccacc acactggcag
     9601 cttgtgtcgc caatggcagt gtaaagttgc ccataccgca gaacagatcc agtacgcgat
     9661 cttcaggttg cacatccagc cattccaacg cacacgctac cattttttgg ttcacacccg
     9721 cattgacctg aataaaatcg cgcgggctaa aagttaagcg caacccgttt gagtcatacc
     9781 agggcatctc accagcgacg gtttcgagta tctcactatc gggggcgaga tacagatcca
     9841 ggccttcaga atgcgaaaag cgttccagtt tttcgcgatc tgccgaactt agcggtgcgg
     9901 tatggcgcaa aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca tgaccaagat
     9961 ggcgcatagc ttgtaagctg cccaggcatg ccctgacttt gggcagcaat gcttcaagtt
    10021 ggggcactaa aatggggcat tgtttaacgt cgacaatgtc actggagccc gctttgcgaa
    10081 agcccatctg aagttgctgt gttttcggta agtagttcaa acttaaacgc gcgcgacggc
    10141 gatagcccca gggaacatcg gcgatcactt cagagacctc gtgtttcatt aatcgggcga
    10201 gtgccgcact tttgcttcgc tgctgtaaat cctcgctggc gtgttgttgc tgacagccgc
    10261 cgcatacgcc aaaatgaggg cagcgtggcg tttcgcgttc cgggctatcg cttaaccggc
    10321 gtacgacttt agcgcgggcg tattgttttt tatcttcagt aacagtaact tccgcgtttt
    10381 cctgtggcaa taatccagga ataaataacg ctttgccgtt atgtcgtgcc acgccctgac
    10441 caaaagagtc gaggtcgttg actgaaacag ttatgatctg acgcgtcgtc gtgcgtcgtt
    10501 ttgcagagta gaattgcgcc attggcgaga ctttctcaat ttaacagtgt gaccttaatt
    10561 gtcccataac ggaactccat gaccaactac agcctgcgcg cacgcatgat gattctgatc
    10621 ctggcaccga ccgtccttat tggtttattg ctgagtatct ttttcgttgt gcatcgctat
    10681 aacgacttgc agcgtcaact ggaagatgcc ggagccagca ttattgagcc gcttgcagtt
    10741 tctactgaat atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt aataagcgta
    10801 ctgcatcgtc gccattccga tattgttcgc gcgatttcgg tttatgatga aaataaccga
    10861 ctctttgtca cctccaattt tcatcttgat ccctcatcaa tgcagctcgg cagcaacgtg
    10921 ccgtttcctc gccagctcac tgtcactcgt gacggcgata ttatgatcct ccgcacgccg
    10981 attatttctg agagttactc ccccgacgaa tcgcccagta gcgatgccaa aaatagtcaa
    11041 aatatgttgg gatatattgc gcttgagctg gatcttaaat cggttcgctt gcagcaatat
    11101 aaagagatct ttatttccag cgtgatgatg ctgttttgta tcggtattgc gcttattttt
    11161 ggctggcgct taatgcgcga tgtaaccggt ccgattcgca acatggtgaa taccgtcgac
    11221 cgcatccgtc gcgggcaact cgacagccgg gtggaaggat ttatgctcgg cgagctggat
    11281 atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg ctgcttatca cgaagagatg
    11341 cagcacaata tcgaccaggc gacgtccgat ctgcgtgaaa cgctggagca gatggaaatt
    11401 cagaacgttg agttggatct ggcgaaaaag cgtgcccagg aagcggcgcg tattaaatcc
    11461 gagtttctgg caaatatgtc acacgagctg cgtacaccac tgaatggtgt tattggcttt
    11521 actcgcctga cgctgaaaac agaattaaca ccaacgcagc gcgatcacct gaatacgatt
    11581 gaacgttcgg caaataattt gctggcaatt attaatgatg ttctcgactt ctcgaaactg
    11641 gaagcaggta agctgattct ggaaagtatt ccattcccac tacgcagcac gctggatgaa
    11701 gtcgttaccc tgctggcaca ttcttctcac gataaagggc tggaactgac gctcaatatt
    11761 aagagcgacg tgcccgataa cgtaattggc gacccactgc gattacagca aatcatcact
    11821 aacctggtag ggaatgcaat taaattcacc gagaatggca acattgatat tctggtagaa
    11881 aaacgtgcgc tgagtaatac caaagtgcag attgaagtgc agattcggga taccggcatt
    11941 ggtattcctg aacgcgatca atcgcgctta ttccaggcct tccgacaggc tgatgccagt
    12001 atttcccgcc gtcatggtgg caccgggctg gggctggtga ttacacaaaa actggttaat
    12061 gaaatgggcg gcgatatttc gttccatagc cagccgaatc gcggttcaac tttctggttc
    12121 cacattaatc tcgatctgaa cccgaacatt attatcgaag ggccatccac ccagtgccta
    12181 gccggcaagc gcctggccta tgtcgaacca aactccgcag cagcgcaatg cacgctggat
    12241 attttaagtg aaacgccgct ggaagtggtt tatagcccaa cgttctccgc gctgcctccc
    12301 gcgcattacg acatgatgtt attaggcatt gcggtgacct tccgcgagcc gctaacaatg
    12361 caacatgagc gattagcgaa agcggtatcg atgaccgatt ttctgatgct ggcacttcct
    12421 tgccatgcac aagtcaatgc tgaaaaactt aagcaagatg gtatcggcgc gtgtctgctg
    12481 aaaccattaa cacctacgcg cctgttacct gccctgacgg aattttgtca tcacaaacaa
    12541 aacacgcttt tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat ggcggttgat
    12601 gacaaccccg ctaacctgaa acttatcggc gcattgctgg aagatatggt gcaacatgtg
    12661 gaactttgcg atagcgggca tcaggcggtt gaacgggcga aacagatgcc gttcgatttg
    12721 atcttaatgg atattcaaat gccggacatg gacggcattc gggcctgcga actcatccac
    12781 cagctccccc atcagcaaca aacgccagtt atcgcggtaa cggcgcatgc aatggccggg
    12841 caaaaagaga agctgcttgg cgcagggatg agcgattatc tggcgaaacc gattgaagaa
    12901 gagcgattgc ataatttgtt gttgcgctac aagcctggca gcggtatttc gtctcgcgtc
    12961 gtgacgcccg aagtcaacga aattgtggtg aacccgaatg cgaccctcga ctggcaactg
    13021 gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca aatgttactc
    13081 gatttcctgc ctgaagttcg caacaaagtt gaggaacagc tggttggaga aaacccggaa
    13141 ggcctggttg atttgattca taaactgcat ggcagttgcg gctatagcgg tgtgccgcgt
    13201 atgaagaatc tctgccagct gatcgaacaa cagctacgta gcggtactaa agaagaagat
    13261 ttggagccgg agctgctgga actgttggac gagatggata atgtcgcgcg cgaagccagc
    13321 aaaattctcg ggtaatggac gtcttgtcga gtcaaaatag gttccgtcca atgccaatgc
    13381 tgggagcagc acagacagtc ccctcgcccc tccggggaga gggttagggt gaggggaaca
    13441 ggccaacgca agtgcgaatg attaccctca ccccgcccct ctccctgaaa gggcgagggg
    13501 gaaaaccggc cccgtaaacc acttcgtcag taatctcaaa ccgacttctt atgaagccgg
    13561 ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg ttatcaaacg tccagccagg
    13621 aatcaggtac tgcattccca tcgcatcgtc acgcgcacca agcccatgtt tctgatacag
    13681 ctcgtgggct ttcatcacct gatccatatc gatttctacg cccaatcctg gtttttgcgg
    13741 cacctgtacc agcccgcctt tgatctcaaa cggttctttg gtcagacgct gattgccttc
    13801 ctgccagatc cagtgcgtat caatagcagt aattttaccc ggtgcagcag cggctacatg
    13861 ggtaaacatc gccagggaaa tatcgaagtg gttgttagag tgtgaacccc aggtcagccc
    13921 aaattcatgg cacatttgcg ccacacgtac cgaaccttgc atcgtccaga aatgcggatc
    13981 cgccagcggg atatcaacgg attgtaacga gagcgtatgg cccatctgcc gccagtcggt
    14041 ggcgatcata ttggttgcgg tcggcaggcc agtcgcgcga cggaactccg ccatcacttc
    14101 acgcccggag aaacattgct ccgcaccaca cggatcttct gcataagcca gcgacccttt
    14161 caggtattta ccaattttaa tcgcttcgtt cagcgaccag gcaccgttag gatcgagcgt
    14221 aatacgcgcc tgcgggaaac gtttcgccag tgccacaata gactcggctt cttcttcccc
    14281 ggccagtaca ccgcctttca gtttgaaatc gttgaagcca tatttttcat acgccgcttc
    14341 cgccaggcgc accaccgcat ccggcgtcat cgcttcttcg tgacgcaggc gataccagac
    14401 acatttatca tccggctggc tttgatacgg cagcggtgtg gctttgcgat taccgacgaa
    14461 gaacagataa ccgagcattt cgacttcgct acgctgttga ccatcgccca gcagggatgc
    14521 cacgttaacc cccagatgct gccccagcag atccagcatt gccgcttcta tcccggtaac
    14581 cacatgaata gtggtccgca ggtcaaatgt ctgcaaaccg cgcccgccgg catcacgatc
    14641 ggcaaatgta ttacgtacca gcgtcagaac gtttttgtat tcacccagcg ttttacccac
    14701 caccagcgga atcgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc
    14761 tacgccagtg tgaccggaat tatctttgat aatcacaata ttacgcgtga agaacggtgc
    14821 atgtgcacca ctcagattca tcagcatact gtcatgaccc gccaccggga tgacctgcat
    14881 ttcagtaaca acaggcgtcg taaattgaga actcatattt atgtcctttt tcagaatcaa
    14941 tgacggccga aaacgggacg tttacggtca aaggtccagc cggggatcag gtactgcatc
    15001 ggacctgcgt cgttacgcgc accgccaggc agacgtttat aggcctcatg tgccttttgt
    15061 acctgttccc agtccagttc cacgcccaga ccgggcgcat caggaactgc aatttttccg
    15121 tttttaatct ccagcggatt ttgggtcagg cgacaatctc cctcctgcca aatccagtgg
    15181 gtatcgatag cggtaggatt acccggtgcc gccgcaccca catgggtaaa catcgccaga
    15241 gagatatcaa agtggttgtt ggaatgacag ccccaggtca gcccccagtc gtcgcaaagc
    15301 tgcgccacgc ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct
    15361 actgcattga gcattaccgc atgacccatt tcacgccagt tggtggcgat catgttagtc
    15421 gcgacgggca agccggtcgc ccgccgaaac tccgccatca cttcacgacc ggagaaaccc
    15481 tgttctgcgc cgcatggatc ttcggcatag gtaagaacat cattcagccc tttgcacaaa
    15541 gaaatggctt catcaagcag ccatgcaccg ttgggatcaa cggtaatccg cgcatccggg
    15601 aagcgtttct tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa cactccgccc
    15661 ttaagtttga aatctttaaa gccataacga tcctgtgaag cttccgccag acgcacaacg
    15721 gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc actcatggtt gcccggcgta
    15781 ttttccagat aaggaagatc ggttttggtc cgatcaccga tataaaacag ataaccgagg
    15841 acggtaatag cctcgcgttg cttgcctggc cccaacagtt cgcagaccgg aacattcagc
    15901 gccttaccta gcaggtcaag caaggcggct tccagcgccg ccacggcatt aacgcgcaat
    15961 tcaaaagtcc aggcaccttt gccgaaggta tcaaaatcgg ctgcctgatt acctttatgc
    16021 acctgctgaa ccactttatt caggcgcgca acttcctggc ccaacaccat cggaatagca
    16081 tcgacaagcg tctgataaat cacctctccg cctggcgctt caccaatgcc ggtatgcccg
    16141 gcgttatcgg tgagtaccac aatattgcga gtgaaatatg cgttatgcgc cccaccaata
    16201 ttaagcaaca tgctatcgtg cccggcgacc ggaatgactt tcatatcagt aataacagga
    16261 ctggattgtg tcgtcatctt attgccccgc gacaggtttc agctcgatac gcttgatatc
    16321 gcccaccagc accaggtagc tcagtaccgc gattaaggca tgaacaccaa cataaatcag
    16381 cgccccatta aacgagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat
    16441 gccagaaatg ttaccgaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg
    16501 cgcggtatct gccattactg cccaacccag cgccccgatg cccttaccga agaaggccag
    16561 cgccataaag ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat
    16621 cgacagcaac atgcccatta cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg
    16681 gcgcatcagc cagtcggaaa taatcccacc cagcacaccg ccgataaaac cgcaaaccgc
    16741 cggaacggaa gccacaaagc ccgctttcag aatcgacatc ccgcgtgcct gcaccagata
    16801 aaccgggaac caggtaataa agaagtaagt cagggcgttg atacagtact gaccgatata
    16861 aacgccgatc atcatccgtg accctagcag ctgtttgatc tgcccccact tcacgctgaa
    16921 cggtacttta actttggtgt tttgctgatc catattgatc agcgcgccac ccgcggcgat
    16981 gtactccagc tctttctgat ttacacccgg atgttgattt ggctcgtgga tgactttcag
    17041 ccagaaaaag ctgatgacaa tgcccagacc gcccataaag aagaagacgt gtgaccagcc
    17101 cacttcatga gtcagccagc ccataatcgg cgcgaagatc accgttgcga agtattgagc
    17161 ggagttaaaa atagacaccg ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat
    17221 gcgactgttg ccggggaaag atggcgcttc agcaagcccg accaggaagc gcagcgtaaa
    17281 cagggcaacg ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa
    17341 catcgaccag ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa
    17401 ccaaccacct gggatctgcc cgataacata agcccatgag aaagcagaga acacatagcc
    17461 cattcccaca ggatcaaggc cgatatcttt ggccatttcc gaaccggcga tagagagcgt
    17521 agcgcggtcg ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg
    17581 agcatttgtg cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact
    17641 cctgaaaatt cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg
    17701 tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat
    17761 atctggcaat agcagtataa aaagcgcgcc atagcggctc accgtgcaac aacacaacat
    17821 taatgcgttc aatgaggccc gattttggca ttagccctgg acggtggaat ccacttcacg
    17881 gaaatgaaaa caagaacaag aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa
    17941 atggatcgct tgactccagg caaacgccag taaaaatccg cgctatgaag cagtttttac
    18001 tggcatttgc ctgaaaagat tcgattcagc accgctaaaa cggcatttac cgctcgctga
    18061 acgtatcagg acagcagcgt gccccaatgt tcgacccacg gatttgattc cgtttccggt
    18121 tccgggttct cactggcgtc aatcaacagc atttcgccaa cccgctgagc gctctgttcc
    18181 tgcaacaagg catcgaactg tttgccacca ttgcagaaat tcacataact actgtcgccg
    18241 agcgcaatca cgccataacg cagattcggc tggaagccca gggtatcttt gattccctga
    18301 aagagcggca caatgctatc aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga
    18361 acatacttat cctgataggg cagccagtcg cttaattcag gatcttcaaa taccgttgct
    18421 ttgtggccct gcgcggtcag aattgcttcc gcttcttccg ccactaacag cgaatttccg
    18481 tacatagtgc cgacaaaaat accaatttcc gccataccgt tttctccctg gattaggaac
    18541 ttatctctcc atcctgaccc gatggcgcac tgaactcaac cctttcattt tcaggaagca
    18601 gaccccgcca gccaaattgt gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa
    18661 tagtcaacgg ctcgccagta aaaggatgcg tcaatgacaa ctgactggca tgcagcatta
    18721 accgctgaag gccaaaatgt tcagcaccgc tgcgattctg gcgtaaatca ccatgtttgc
    18781 tgtcgccaat aatcgggtgg cgcaaatggg caagatggcg acggagctgg tgcttacgtc
    18841 cggttttcgg ctccagttcc accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta
    18901 ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac tgctggctgt gggcctttat
    18961 cttcgcgggc aaatttatca gcgattttgt ccagttcttc caccagtgga taatccagaa
    19021 ctgcttcttc catcaaccag ccgcgcacaa tcgcatggta acgtttctgg atttggtgct
    19081 gttcaaactg ttgtgccagc agccgtccgg cctcgctgga taatcccatc aacaacacac
    19141 cagaagtggg tcggtccaga cgatgagcag taaacacatg ctggcctatc tggtcacgca
    19201 cggtttgcat gaccactact ttctcgtcgc gatccagcca gctacggtga accagccagc
    19261 cggagggttt atttaccgca accagccatt catcctgata gagtatttcc agcattagct
    19321 cgcatcatcc gcaaaaagag catccagttt ttccagctca gccagaataa gcgcgcgttg
    19381 cgggtggtcc gtcgccagcg ccatttcata atagggagca acggcaaaag cgcccggtaa
    19441 cggctggttg ttttctaaca aatcgtgcat tcgcgggatc agcacccact gcaaccactc
    19501 cagtggttcc atggtgtcca taaagaacgg ttgggtactg ttaaattgat gcggctgggg
    19561 ttcatcgttt tgccagtgct gatgttcacg cagtaatgct tcaagcgcct gcagctggag
    19621 acgaacgcgg tcatgagtgg tcataaagca acctcaataa gaaaaactgc cgcgaaggat
    19681 agcaggaata aaaaaaggga gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt
    19741 ccagctactt ttgttgctcc ctgctcatcc ttgacaactt ttcctctggc cttgcggcca
    19801 atcgttcatc ctgaactatt gcttcctgct cacaccaccc cgatgtgata cttcatcctg
    19861 aagtgtccct ggccttcctg acccaccgaa tcatcctgac cggttctcat tctccatcct
    19921 ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct tcatccagaa gcctttccct
    19981 gaaacaccat cctggtgtgt ccttcagaag tgtcatcatc ctgatgttca cttcgttgtc
    20041 tgactccctg tcgacgaaga tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca
    20101 gacaatgcct taaggaaaat ttcatatgaa agttaaggat ttacaagcaa tatattgatt
    20161 ttaaatgaaa ataacgattc ttatcttttt aagtaatcgc ttatttcctg gcgatctcct
    20221 acagagtctg taagagatct ctcacaacca gatggggtat ttggattaca gaagaggctt
    20281 aagttgatta aggaattccg caagatttga ggctaaatgc gcccgtttac gtgttcccag
    20341 cgtctcttta cacacttcac cggagagatt acacaccgag atcacctcta actcttcttc
    20401 cagcgtcgcg ataaatagcg tgggcggcaa ttttagcctt ttctgagtca ccagatgacc
    20461 aatcagattt tcctgcaccc gacggaagtc gtcttcactc caggtttgca gcaatgtcag
    20521 tttaatatcg ccaaactgcg cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg
    20581 aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc
    20641 ggtaaacggc tggggttgcc agtatacggc gtcttcggtg gttgaaataa tgcacggtga
    20701 aggaacgccg tacagttcct cactcaacgg ccagctttta tgctcttcat gccacgcatc
    20761 gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc
    20821 tcttcactta aaccagatac acttgtcgtt tagtttatct ggtttatgac ggtgaaacat
    20881 gtcttcttat gcaaaccatc aggcacttgc gggcctgact cttggaaaat caaccgatta
    20941 ccgggatacc tatgacgcca gcctactgca aggcgttccg cgtagtctga atcgcgatcc
    21001 gctgggtctg aaagcggata acctgccttt tcacggtacg gatatctgga cgctatatga
    21061 actttcctgg ctgaatgcga aaggtttgcc gcaggtcgcc gtcggtcatg ttgaacttga
    21121 ttacaccagc gcaaatctga ttgagtcgaa gagttttaag ctctatctca acagttttaa
    21181 ccagacgcgt tttaataact gggatgaggt gcgccagacg ctggagcgcg acttaagcac
    21241 ctgcgctcag ggtaaggtta gcgtggcgtt atatcgtctt gatgaactgg aaggccaacc
    21301 gataggtcat ttcaatggca cttgcattga tgaccaggat atcactatcg ataactatga
    21361 attcactact gactatctgg agaatgccac cagtggtgaa aaagtagtgg aagagacgct
    21421 tgtcagccac ctgctgaaat caaactgcct gatcacccat caaccagatt ggggttcgat
    21481 ccaaattcag tatcgtggac gccaaattga cagagaaaaa ctgctgcgtt acctggtttc
    21541 tttccgccat cacaacgagt tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt
    21601 acgcttctgc cagccagaga aattgagcgt ttacgcacgt tatacccgtc gtggcggtct
    21661 ggacattaac ccgtggcgca gtaatagcga ttttgtccca tcgaccacaa gactggttcg
    21721 gcaataaatt ttttctcaat tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat
    21781 caggcagggc tattgtaatc aaagggaatg acgatattcg tcccataagg agtttttctt
    21841 gattacacat attagcccgc ttggctccat ggatatgttg tcgcagctgg aagtggatat
    21901 gcttaaacgc accgccagca gcgacctcta tcaactgttt cgcaactgtt cacttgccgt
    21961 actgaactcc ggtagcttga ccgataacag caaagaattg ctgtctcgtt ttgaaaattt
    22021 cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg gaactgatta atcccccgga
    22081 agaggctttt gtcgatgggc gaattattcg cgctttgcag gccaacttgt tcgcggttct
    22141 gcgtgacatt ctcttcgttt acgggcaaat ccataacacc gttcgttttc ccaacctgaa
    22201 tctcgacaac tccgtccaca tcactaacct ggtcttttcc atcttgcgta acgctcgtgc
    22261 gctgcatgtc ggtgaagcgc caaatatggt ggtctgctgg ggcgggcact caattaacga
    22321 aaacgaatat ttgtatgccc gtcgcgtcgg gaatcagctg ggcctgcgtg agctgaatat
    22381 ctgcaccggc tgtggtccgg gagcgatgga agcgccaatg aaaggtgctg cggtcggaca
    22441 cgcacagcag cgttacaaag acagtcgttt tattggtatg acagagccgt cgattatcgc
    22501 cgctgaaccg cctaacccgc tggtcaacga attgatcatc atgccggata tcgaaaaacg
    22561 tctggaagcg tttgtccgta tcgctcacgg tatcattatc ttccctggcg gtgtgggtac
    22621 ggcagaagag ttgctgtatt tgctgggaat tttaatgaac ccggccaaca aagatcaggt
    22681 tttaccattg atcctcaccg gcccgaaaga gagcgccgac tacttccgcg tactggacga
    22741 gtttgtcgtg catacgttgg gtgaaaacgc gcgccgccat taccgcatca tcattgatga
    22801 cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg
    22861 cgacacaggc gatgcctaca gctttaactg gtcaatgcgc attgcgccag atttgcaaat
    22921 gccgtttgag ccctctcacg agaatatggc taatctgaag ctttacccgg atcaacccgt
    22981 tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa
    23041 agaagtcggt attcgcgcca ttgaagagtt tggtccttat aaaatcaacg gcgataaaga
    23101 gattatgcgt cgtatggatg acctgctaca gggttttgtt gcccagcatc gtatgaagtt
    23161 gccaggctca gcctacatcc cttgctacga aatctgcacg taatctccct cccccggtga
    23221 cgtttgttgc cgggttttct cgtttttggt cacataccca tcaatttatt tcatttgtta
    23281 tatgaatgtt tcttaccacc ctcacgcgat aaatatcatt acagttaata tgtcatacaa
    23341 tttatgtcgt aacgcaaacg tttccctatt ttcataaacc acattttatc gctacgaaaa
    23401 agaaattatc gccatgatta actaaaagta ttgatttttt cagttcaacc tacatatatt
    23461 gcgcgccccg gaagaagtca gatgtcgttt aatgggcaaa tattgccctt aaattctctt
    23521 ttacttttga tttacagagt aaagcgttgg gataatctat cttccaagta gattattgta
    23581 tttgggatca agatcactga tagatacata acttgtgtgt atctttccgc cctcaaatta
    23641 ttacggcggt aaatgattaa gccatcgccg atagacagat ttcattttta cggtcaggca
    23701 ccttcccggg ctgaactggc taaaagctga attatttgca ttcctccagg agaaatagat
    23761 ggaaacgact caaaccagca cgattgcgtc gaaagactct cgtagtgcct ggcgcaagac
    23821 agacaccatg tggatgctgg gcctttacgg cacggcaatc ggcgcgggcg tgctgttcct
    23881 gccaatcaac gccggtgttg gcggtatgat cccgctgatc atcatggcta tccttgcgtt
    23941 cccgatgaca ttttttgctc accgcggcct gactcgcttc gtactgtctg gtaaaaaccc
    24001 aggcgaagac atcaccgagg ttgtagaaga acactttggt attggcgcag gtaaactgat
    24061 taccctgctc tacttcttcg ctatctaccc gatcctgctg gtttatagcg tggcaatcac
    24121 caatacagtt gaaagcttca tgtctcacca gctgggtatg acaccaccgc cgcgtgcgat
    24181 tctgtcgctg atcctgatcg tgggtatgat gaccatcgtt cgcttcggtg agcagatgat
    24241 cgttaaagcg atgagtattc tggtattccc gtttgttggc gtactgatgc tgctggctct
    24301 gtacctgatc ccgcagtgga acggcgcagc actggaaacg ctgtctctgg acactgcatc
    24361 ggcaaccgga aacggtctgt ggatgaccct gtggctggca attccggtaa tggtgttctc
    24421 gttcaaccac tctccgatca tctcttcttt cgccgttgcg aagcgtgaag agtacggcga
    24481 tatggcagaa cagaaatgtt cgaagatcct ggcattcgca cacatcatga tggtgctgac
    24541 cgtaatgttc ttcgtcttca gctgcgtact gagcctgact ccggcagacc tggctgcggc
    24601 taaagagcag aacatctcga ttctgtctta cctggctaac cactttaacg caccggttat
    24661 cgcgtggatg gctccgatta tcgcgattat cgctatcacc aaatccttcc tcggccacta
    24721 cctgggcgca cgtgaaggct tcaacggtat ggtgattaaa tctctgcgtg gtaaaggtaa
    24781 gtctatcgaa atcaacaagc tgaaccgtat cactgcgctg ttcatgctgg taacgacctg
    24841 gattgttgcc accctgaacc cgagcatcct gggtatgatt gaaaccctgg gcggcccaat
    24901 catcgcgatg atcctgttcc tgatgccgat gtacgcaatt cagaaagtac cggcaatgcg
    24961 taagtacagc ggtcacatca gcaacgtatt cgttgtcgtg atgggtctga ttgcaatctc
    25021 cgcaatcttc tactctctgt tcaactaagt cctttcgcgc cgctttcggg cggcgcttcc
    25081 tccgttttaa cgcgatgtat ttcctatgat tagcgtattc gatattttca aaatcggcat
    25141 tggcccttcc agttctcata ccgttggacc aatgaaagcg ggtaaacaat ttaccgacga
    25201 tctgattgcc cgtaacctgc ttaaagacgt gacccgcgtg gtggttgacg tgtacggctc
    25261 gctctctctg actggtaaag gccaccacac tgatatcgcc attattatgg gcctggcggg
    25321 taacctgccg gataccgtgg atatcgattc catccctggt tttattcagg atgtgaatac
    25381 tcatggtcgc ctgatgctgg caaatggtca gcatgaagtg gagttcccgg ttgatcagtg
    25441 catgaacttc catgccgaca acctttctct gcatgaaaac ggtatgcgca ttaccgcgct
    25501 ggcgggcgat aaagtcgttt acagccagac ttattactct atcggcggtg gctttattgt
    25561 tgatgaagaa cattttggtc agcaggatag cgcaccggtt gaagttcctt atccgtacag
    25621 ttcagctgcc gatctgcaaa aacattgtca ggataccggg ctgtcactct ctggcctgat
    25681 gatgaaaaac gagctggcgc tgcacagcaa agaagagctg gaacagcacc tggccaacgt
    25741 ctgggaagtc atgcgtggcg gtattgagcg cggtatttcc accgaaggcg tgttgcctgg
    25801 caaactgcgc gttccacgcc gtgctgcggc actacgccgg atgctggtca gccaggataa
    25861 aaccaccact gacccgatgg cggttgttga ctggatcaac atgtttgcac tggcagtgaa
    25921 cgaagagaac gctgctggcg gtcgcgtggt gactgcgccg actaacggtg cgtgcgggat
    25981 tatcccggct gtgctggcgt actacgataa gtttatccgc gaagtgaacg ctaactcact
    26041 ggctcgttac ctgctggtag ccagcgccat tggttctctt tataagatga acgcgtcgat
    26101 ttctggtgcc gaagtgggtt gccagggtga agttggcgtg gcgtgctcta tggcggcagc
    26161 tggtctggca gaactgttag gcgcaagccc ggcgcaggtg tgcatcgcgg cggaaatcgc
    26221 catggagcat aacctcggtc tgacgtgtga cccggttgct ggacaggtgc aggtaccgtg
    26281 catcgagcgt aacgccattg cggcagtaaa agcggtgaac gccgcgcgta tggcgctgcg
    26341 ccgtaccagc gagccgcgcg tctgcctcga taaagttatc gagaccatgt acgaaacagg
    26401 taaagatatg aacgccaagt accgcgaaac ctctcgcggt ggcctggcaa tgaagatcgt
    26461 tgcctgcgat taatctctcc cggaggcctc gttttgcgag gcctcttccc gttttctcaa
    26521 ccagccatag cctgttccgg catcgaatgt tacccttatc gcctgatctt taagggggtt
    26581 atcgtggctg ttcatttgct tattgtcgat gcactgaatc ttattcgtcg cattcatgcc
    26641 gttcagggtt cgccctgtgt cgaaacctgc cagcacgcgc tcgatcagct cattatgcac
    26701 agccaaccaa cccacgcggt cgccgttttt gatgatgaaa accgcaatag cggctggcgt
    26761 catcagcgtt taccagatta caaagcgggt cgaccgccaa tgccggaaga gttgcacgac
    26821 gagatgcctg cactacgcac cgcctttgag caacgcggcg tcccgtgctg gtcagccagc
    26881 ggtaacgaag ccgatgactt agccgccacg ctggcggtca aagtgacaca ggccgggcat
    26941 caggcaacga ttgtttcgac agataaaggc tactgtcagt tactttcacc gacattacgt
    27001 attcgtgatt acttccagaa acgttggctg gatgcgccat ttatcgataa agaatttggc
    27061 gttcaaccgc agcagttgcc cgattactgg gggcttgcgg ggatcagcag ttcaaaggta
    27121 ccgggtgttg cggggatcgg acccaaaagc gccacgcagc tgctggtcga gtttcagagt
    27181 ctggaaggga tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa aaaattagaa
    27241 acccataaag agatggcgtt tctgtgccgc gatattgccc gcttacaaac cgatttgcat
    27301 atcgacggca atttacagca attgcggttg gtaaggtaac ggcgtgccgg atacggcgca
    27361 aacgtcgcat ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac
    27421 aatatcctca agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc
    27481 ctgcgccagt gccggaatgt cttccttgcg taccccaaca tcacgcaaat gtggcggaat
    27541 accgacatca cggttgagag caaacacagc ttcaacagcg gcattacgcg cctcttccag
    27601 gctcatacct tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt
    27661 aaagtcagcg ttatagcgca tgacatgcgg taacaggatg gcgttcgcga caccgtgtgg
    27721 agtgttataa aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt
    27781 cgagaagccc atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc
    27841 accagcaacc gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc
    27901 atcggttagc gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc
    27961 gacacccgtc gcagctttca gcgctggagg cataccatcc atcatgtcag cgtcaataaa
    28021 cgccacttgc gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc
    28081 agtgatcacg tagttaatgg tcacttctgc cgcagtgcct gctgtggtgg ggattgccag
    28141 aatcggtaca ctgggtttat tggtcgggga aagtccttcc aggctacgca catcggcaaa
    28201 ctccgggttg ttgctgataa tgccaatcgc tttacaagta tcctgtggag aaccaccacc
    28261 aatagcgatc aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac
    28321 agtaattgtt gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc
    28381 catcttatcg gtcacttttg ccaccacgcc gcattgcacc agcgttttat cggtgacgat
    28441 cagcgccttc tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg
    28501 accaaaccat gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc
    28561 tttacgaaat tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc
    28621 aatctcttca tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag
    28681 ttgcgccagc acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca
    28741 agcgataagc ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac
    28801 atgttcagaa agttcacgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc
    28861 gccagccgcc gcaatcatgt agtgaatagc ggggattggt cggttaagaa tggaaactgc
    28921 cgtgcaatga acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc
    28981 cgccatatgg aaacgccatt cgcttgaggg gagctttcct tcctcatgt
//
LOCUS       DARNVM010000046        28900 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.046, whole genome shotgun sequence.
ACCESSION   DARNVM010000046 DARNVM010000000
VERSION     DARNVM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.046"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(47..1297)
                     /gene="icd"
                     /locus_tag="SI621_001814"
     CDS             complement(47..1297)
                     /gene="icd"
                     /locus_tag="SI621_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEI4064541.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWMKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMDGAKLLKCSEFGDAIIKNM"
     gene            1879..2328
                     /locus_tag="SI621_001815"
     CDS             1879..2328
                     /locus_tag="SI621_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064542.1"
                     /translation="MDTTEERNGTYFYRGHPNLSKIELLNVIFLEEFCKELGIDSVSG
                     AAILSGQPWLGTRAKPQGATLGTSVVSKYARILMRDARMPFGISVHTPVGVRMQKSNK
                     LAAIIARWVPWIGWASLSMSLYRVSTRTQEKYNLIAKPQDRIKWTSF"
     gene            2313..2663
                     /locus_tag="SI621_001816"
     CDS             2313..2663
                     /locus_tag="SI621_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HEI4064543.1"
                     /translation="MDFLLMATTITDEVLALFRDEIPGYQDDHGKEIPLELDSDLFDY
                     PRDDLEDAIRKYKERFGINLDNLDWSLYLPWESAPLLQRWFKLKREEVETTRLPLTVR
                     MFAESAEAGKWLYQ"
     gene            2996..3634
                     /gene="rluE"
                     /locus_tag="SI621_001817"
     CDS             2996..3634
                     /gene="rluE"
                     /locus_tag="SI621_001817"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HEI4064544.1"
                     /translation="MKYFSDSKSSFRKKQVSRLNSQHSTKRKPENQPTRVILFNKPYD
                     VLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGK
                     IYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRERKSIPT
                     SWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            3644..4105
                     /gene="nudJ"
                     /locus_tag="SI621_001818"
     CDS             3644..4105
                     /gene="nudJ"
                     /locus_tag="SI621_001818"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HEI4064545.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEANETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSRQRYPLEMIGDFNWPFTKGVI"
     gene            4159..5265
                     /gene="mnmA"
                     /locus_tag="SI621_001819"
     CDS             4159..5265
                     /gene="mnmA"
                     /locus_tag="SI621_001819"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEI4064546.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            5301..5942
                     /gene="hflD"
                     /locus_tag="SI621_001820"
     CDS             5301..5942
                     /gene="hflD"
                     /locus_tag="SI621_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEI4064547.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5946..7316
                     /gene="purB"
                     /locus_tag="SI621_001821"
     CDS             5946..7316
                     /gene="purB"
                     /locus_tag="SI621_001821"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEI4064548.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            7485..8156
                     /gene="phoP"
                     /locus_tag="SI621_001822"
     CDS             7485..8156
                     /gene="phoP"
                     /locus_tag="SI621_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HEI4064549.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            8156..9616
                     /gene="phoQ"
                     /locus_tag="SI621_001823"
     CDS             8156..9616
                     /gene="phoQ"
                     /locus_tag="SI621_001823"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HEI4064550.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            9692..10813
                     /gene="roxA"
                     /locus_tag="SI621_001824"
                     /gene_synonym="ycfD"
     CDS             9692..10813
                     /gene="roxA"
                     /locus_tag="SI621_001824"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HEI4064551.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(10862..12088)
                     /gene="pepT"
                     /locus_tag="SI621_001825"
     CDS             complement(10862..12088)
                     /gene="pepT"
                     /locus_tag="SI621_001825"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEI4064552.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            12338..13474
                     /gene="potA"
                     /locus_tag="SI621_001826"
     CDS             12338..13474
                     /gene="potA"
                     /locus_tag="SI621_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HEI4064553.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            13458..14315
                     /gene="potB"
                     /locus_tag="SI621_001827"
     CDS             13458..14315
                     /gene="potB"
                     /locus_tag="SI621_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HEI4064554.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            14312..15106
                     /gene="potC"
                     /locus_tag="SI621_001828"
     CDS             14312..15106
                     /gene="potC"
                     /locus_tag="SI621_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HEI4064555.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            15103..16149
                     /gene="potD"
                     /locus_tag="SI621_001829"
     CDS             15103..16149
                     /gene="potD"
                     /locus_tag="SI621_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HEI4064556.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            16207..16668
                     /locus_tag="SI621_001830"
     CDS             16207..16668
                     /locus_tag="SI621_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEI4064557.1"
                     /translation="MSQDSRVFFHIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            16665..17450
                     /locus_tag="SI621_001831"
     CDS             16665..17450
                     /locus_tag="SI621_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEI4064558.1"
                     /translation="MKKIIILLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGQVESGLDDKHLGLLVENHTQAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTILGLFAWAVAFIVFTAFFYYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSLAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(17588..18409)
                     /gene="cobB"
                     /locus_tag="SI621_001832"
     CDS             complement(17588..18409)
                     /gene="cobB"
                     /locus_tag="SI621_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEI4064559.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(18425..19336)
                     /gene="nagK"
                     /locus_tag="SI621_001833"
     CDS             complement(18425..19336)
                     /gene="nagK"
                     /locus_tag="SI621_001833"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEI4064560.1"
                     /translation="MYYGFDIGGTKIALGVFDSVRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADKRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(19365..20609)
                     /gene="lolE"
                     /locus_tag="SI621_001834"
     CDS             complement(19365..20609)
                     /gene="lolE"
                     /locus_tag="SI621_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HEI4064561.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(20609..21310)
                     /gene="lolD"
                     /locus_tag="SI621_001835"
     CDS             complement(20609..21310)
                     /gene="lolD"
                     /locus_tag="SI621_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEI4064562.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(21303..22502)
                     /gene="lolC"
                     /locus_tag="SI621_001836"
     CDS             complement(21303..22502)
                     /gene="lolC"
                     /locus_tag="SI621_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HEI4064563.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            22763..23836
                     /locus_tag="SI621_001837"
     CDS             22763..23836
                     /locus_tag="SI621_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEI4064564.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            23964..27410
                     /gene="mfd"
                     /locus_tag="SI621_001838"
     CDS             23964..27410
                     /gene="mfd"
                     /locus_tag="SI621_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEI4064565.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSVKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            27554..28516
                     /gene="ldtC"
                     /locus_tag="SI621_001839"
     CDS             27554..28516
                     /gene="ldtC"
                     /locus_tag="SI621_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HEI4064566.1"
                     /translation="MMIKTHFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(28599..28856)
                     /gene="bhsA"
                     /locus_tag="SI621_001840"
     CDS             complement(28599..28856)
                     /gene="bhsA"
                     /locus_tag="SI621_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HEI4064567.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         7127 a         7415 c         7275 g         7083 t
ORIGIN      
        1 aacgggcgta taacacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactct gaacatttca gcagtttagc gccatccatc agacgctcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgcagca tcatctcagc ggagagaata atagagcctg
      241 ggtttacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcgt cagcaatcac gtctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcatcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccagt ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt agcaattgcg tattcgatcg
      661 ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg cttaataccg ataccgcaat
      721 gttccgggaa gcgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtcagct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcaggat gtttaaccgg gcttggggtg ccctgatagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 aacgaatacc gccaccaacc ggagtggtca gagggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gccgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcatta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagac caaagacagt atttttcaga ccttcaaata aggcattaaa
     1561 tatcaataag ttacaccagt ttttaagcaa taaaaaaaac caccagagat aactaacagg
     1621 atgctatttg gtcaaaattt gacattactc gactcagcga ctatctcact ttgagcacta
     1681 ttgggaaaat tatcaaaagt catggttgaa gcgacaagca acgagtaaat tatggcatcc
     1741 cccctttttg aggttcacat gaaacgcttt gcactgatag ctgtgaaact cgttgccttt
     1801 actggttctg catctgctgc aaccgaagaa atcaacttca agtaacagaa acactatata
     1861 acaatggagt gttgacaaat ggatactacc gaagaacgca atggaacata tttctacaga
     1921 ggacacccga acctgtccaa aatagaacta ctcaacgtca tattccttga agaattctgt
     1981 aaggaattgg ggattgattc ggtatcaggt gcagcaatct tatctggtca accgtggctg
     2041 ggtacaagag cgaagccgca aggggcaacc ctgggaacaa gtgttgtctc aaagtatgcg
     2101 cgtatactga tgagagatgc acgcatgcct tttggtattt ctgttcatac accagtgggt
     2161 gtaaggatgc agaaatctaa taagcttgct gcaattatcg ctcgttgggt cccgtggatt
     2221 ggttgggctt cactgtcgat gagtctttac agggtttcga ctcgtaccca ggaaaaatac
     2281 aatctgattg ccaaacctca agaccgtatt aagtggactt ccttctaatg gcgacaacaa
     2341 tcacagacga agttttagca ctgttccgtg atgagattcc aggttatcag gatgaccacg
     2401 ggaaggaaat tccgttagaa ctggactctg atttgttcga ctatccacga gatgatttag
     2461 aggacgctat acggaaatac aaagagaggt ttgggattaa cctggataat ctggactggt
     2521 ctttgtacct tccatgggag tctgcaccat tgcttcaacg atggttcaag ctcaagcggg
     2581 aagaggtaga aacaacacgt ttaccgttaa ctgtacggat gtttgcagaa tcagcagaag
     2641 cgggaaaatg gctgtatcag tagaataaaa aagaaggctg cgatgcccct cttattactg
     2701 ctgatgatgg tagtggtaaa gcatcatcct gaacttgaac agtgaagtgc tgtcacccat
     2761 cagtaataca gaaacactat ttttcatacc agtatttatt caattcatca atgtaaaatg
     2821 tgttcattgg gccatattta tcaccatgca gccacacaac cgctgctcac taacccaggt
     2881 attgatgaaa ccgcgctgaa gagcctgaac cctgaacctc taacgagtac cttaaaagtc
     2941 caatgcaatc cgacactgtg ctaccctctg cgtatagctc tatttggaat ttaccatgaa
     3001 atactttagc gattctaaat cttcttttag aaaaaaacag gttagtcgac taaactcgca
     3061 acactccact aagcgtaaac ctgaaaacca gcccacgcgc gtgatcctgt tcaataaacc
     3121 ctacgatgtt cttccgcagt tcaccgatga agccggacgc aaaacattaa aagaattcat
     3181 cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc gatagcgaag ggttactggt
     3241 gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag ccgggtaaac gcaccggaaa
     3301 aatctattat gtgcaggtgg aaggtattcc cacacaagac gcacttgaag ccttgcgcaa
     3361 tggcgtaacc ttaaatgatg gccctaccct gcccgcaggc gtagagctgg ttgacgaacc
     3421 cgcgtggtta tggccgcgga atccaccaat tcgtgaacgc aaaagtattc ccaccagctg
     3481 gctgaagatc accttatatg aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt
     3541 tggtttcccc acgctgcgac tgattcgcta tgcgatgggt gattactctt tggataatct
     3601 tgccaacggc gaatggcgag aagcgacaga ttaaggacgc aaaatgttta aaccgcacgt
     3661 taccgttgct tgcgtggtgc acgcagaagg caaattttta gtcgttgaag agacgattaa
     3721 tggtaaagcg ttatggaacc aacctgccgg gcatctggaa gccaatgaaa ccttagtgga
     3781 agccgccgcc cgtgagctgt gggaagaaac cggcatcagc gcgcagccgc aacactttat
     3841 tcgtatgcat cagtggattg cgccagataa aacgccgttt ttgcgtttcc tgtttgctat
     3901 tgagcttgag caaatatgcc cgacccagcc tcatgacagc gatatcgact gctgccgttg
     3961 ggttagcgcc gaagaaattt tacaggcgtc aaatctgcgt tcgccgctgg tggcggaaag
     4021 tattcgttgt tatcaaagca ggcaacgcta tccgctagag atgattggcg attttaactg
     4081 gccttttaca aagggtgtca tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc
     4141 aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc
     4201 ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac agggatatca ggtcgaaggc
     4261 ctgtttatga agaactggga agaagacgac ggtgaggaat attgcacagc ggcagcggat
     4321 ctggctgatg cccaggctgt ctgcgacaag ctcggcattg aactgcacac cgttaacttt
     4381 gctgccgagt actgggacaa cgtcttcgaa ctgttccttg ccgaatataa agccggtcgc
     4441 acgccgaatc cggatattct gtgcaacaaa gagatcaaat ttaaagcctt cctcgaattt
     4501 gccgctgaag atttaggtgc cgattatatc gctaccggtc attacgtacg tcgggcagat
     4561 gtcgatggca agagccgcct gctgcgtggt ctggacagca ataaagacca gagctacttc
     4621 ctttatacgc tcagccatga gcagattgcg caaagcctgt tcccggtcgg cgaactggaa
     4681 aaaccgcagg tgcgtaagat tgctgaagat ctcggtctgg tcaccgcgaa gaaaaaagac
     4741 tctaccggca tttgcttcat tggcgagcgt aaattccgcg agttcctggg ccgttatctc
     4801 ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg aaattggtga gcaccagggg
     4861 ctgatgtatc acaccctcgg tcagcgtaaa ggtctgggta tcggtggcac caaagacggc
     4921 accgaagaac cgtggtatgt ggtcgataaa gacgtcgaaa acaacattct gattgttgcg
     4981 cagggccatg aacacccgcg tctgatgtct gtcggtctga ttgcccagca gctgcactgg
     5041 gtcgatcgcg agccgttcac cggtactatg cgttgcacag taaaaacccg ctatcgccag
     5101 accgacatcc cgtgcaccgt caaggcgctg gacgatgatc gcattgaagt gattttcgat
     5161 gagccggttg ccgccgtgac gccgggccag tctgccgttt tctataacgg tgaagtgtgc
     5221 ctcggtggcg gtattattga gcagcgtctg ccgctgccgg tctgattatt atctttactt
     5281 aaaacaagga agcagtgaac gtggcaaaga attactatga catcaccctc gccctagccg
     5341 gtatttgtca gtcggcacgc ctggtgcaac aactcgctca ccaggggcat tgtgatgccg
     5401 atgcgctaca cgtctcactc aacagtatta ttgatatgaa ccccagctcg acgctggcgg
     5461 tttttggcgg cagcgaagcc aacctgcgcg tcgggctgga aaccttgctc ggcgtgctca
     5521 atgccagcag tcgccagggc ttaaacgctg agttaacccg ctacacactc agcctgatgg
     5581 tgcttgagcg caaactctcc tcagcgaaag gtgcgctcga cactctgggc aaccggatca
     5641 acggcctgca acgccagctc gaacacttcg atttacagtc cgaaacgctg atgagcgcga
     5701 tggctgctat ctatgttgat gtgattagcc cgcttggccc gcgcattcag gtaaccggtt
     5761 cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag
     5821 gcattcgcgc cgccgtgctc tggcaccagg ttggcggcgg acgtctgcaa ctgatgtttt
     5881 ctcgtaatcg cctgaccact caggcaaaac aaattcttgc tcatttaacc ccggagttgt
     5941 gatctatgga attatcctca ctgaccgccg tttcccctgt cgatggacgc tacggcgata
     6001 aagtcagcgc gctgcgcggg attttcagcg aatatggttt gctgaaattc cgtgtacaag
     6061 ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc gatcaaggaa gttcctgctt
     6121 ttgctgccga cgcaatcggt taccttgatg caattgtcgc cagtttcagc gaagaagatg
     6181 ccgcacgcat caaaaccatc gagcgtacca ctaaccacga cgttaaagcg gttgagtatt
     6241 tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc ggtttctgaa ttcatccact
     6301 ttgcctgtac ttcggaagat atcaataacc tctcccacgc attaatgctg aaaaccgcgc
     6361 gtgatgaagt gatcctgccg tactggcgtc aactgattga cggcattaaa gatctcgccg
     6421 ttcagtatcg tgatatcccg ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa
     6481 ccatcggtaa agagatggca aacgtcgcct accgtatgga gcgccagtac cgccagctta
     6541 atcaggtgga aattctcggc aaaatcaacg gcgcggtcgg taactataac gcccacatcg
     6601 ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta
     6661 ttcagtggaa cccgtacact acccagatcg aaccgcacga ctacatcgcc gagctgtttg
     6721 attgcgttgc gcgcttcaac accattctga tcgactttga tcgtgacgtc tggggttata
     6781 tcgcccttaa ccacttcaaa cagaaaacta ttgctggtga gattggttct tccaccatgc
     6841 cgcataaagt taacccgatc gacttcgaaa actccgaagg gaacctgggc ctttccaacg
     6901 cggtgttgca gcacctggca agcaaactgc cagtttcccg ctggcagcgt gacctgaccg
     6961 actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta tgcgctgatt gcgtatcaat
     7021 ccaccttgaa aggcgtgagc aaactggaag tgaaccgtga ccatctgctg gatgaactgg
     7081 atcacaactg ggaagtgctg gctgagccaa tccagacagt tatgcgtcgc tatggcatcg
     7141 aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa gcgcgttgac gccgaaggca
     7201 tgaagcagtt tatcgacggt ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcaa
     7261 tgacgccggc taactacatt ggtcgcgcca tcaccatggt tgatgagctg aaataagcct
     7321 caaaacagtg ccggatggcg atgctgtccg gcctgcttat taagattatc cactttttct
     7381 tttttcactt tacctcccct ccccgctggt ttatttaatg tttaccccca taaccacata
     7441 atcgcgttac actattttaa taattaagac agggagaaat aaaaatgcgc gtactggttg
     7501 ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca gattcaggat gctggtcatc
     7561 aggtcgatga cgcagaagat gccaaagaag ccgattatta tctcaatgaa catttaccgg
     7621 atattgcgat tgtcgatctg ggattgccag acgaggacgg tctgtcactg attcgccgct
     7681 ggcgtagcaa tgatgtttca ctgccgattc tggtattaac cgcccgtgaa agctggcagg
     7741 acaaagtcga agtattaagt gctggcgctg atgattacgt gactaaacca tttcatattg
     7801 aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa tagcggtctg gcttcacagg
     7861 tcatttcgct acccccgttt caggttgatc tctctcgccg tgaattatct attaatgacg
     7921 aagtgatcaa actgaccgcg ttcgaataca ccattatgga aacgttgata cgcaataatg
     7981 gcaaagtggt cagcaaagat tcgttaatgc tacaactcta cccggatgcg gagctgcggg
     8041 aaagtcatac catagatgta ctgatgggac gtctgcgcaa aaaaatacag gcacaatatc
     8101 cgcaagaagt gattaccacc gttcgcggcc agggctatct gttcgaattg cgctgatgaa
     8161 aaaattactg cgtctttttt tcccgctctc gctgcgggta cgttttctgt tagcaacggc
     8221 agcagtagta ttggtgcttt cgcttgccta cggaatggtt gcgctgatcg gttatagcgt
     8281 cagtttcgat aaaaccacgt ttcggctgtt acgtggcgag agcaatctgt tctataccct
     8341 tgcgaagtgg gaaaacaata agttgcatgt cgagttaccc gaaaatatcg acaagcaaag
     8401 ccccaccatg acgctaattt atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt
     8461 gccctggctg atgaagatga tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat
     8521 tgaagcggat gttaacgata ccagcctctt gctgagtgga gatcattcga tacagcaaca
     8581 gttgcaggaa gtgcgggaag atgatgacga cgcggagatg acccactcgg tggcggtaaa
     8641 cgtctacccg gcaacatcgc ggatgccaaa gttgaccatt gtggtggtgg ataccattcc
     8701 ggtggagcta aaaagttcct atatggtctg gagctggttt atctatgtgc tctcagccaa
     8761 tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg tggagtttac gccccatcga
     8821 agccctggca aaagaagtcc gcgaactgga agaacataac cgcgaattgc tcaatccagc
     8881 cacaacgcga gaactgacca gtctggtacg aaacctgaac cgattgttaa aaagtgaacg
     8941 cgaacgttac gacaaatatc gtacgacgct caccgacctg acccatagtc tgaaaacgcc
     9001 actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt gagaagatga gcgtcagtga
     9061 tgctgaaccg gtaatgctgg agcaaatcag ccgcatttca cagcaaattg gctactacct
     9121 gcatcgtgcc agtatgcgcg gtgggacatt gctcagccgc gagctgcatc cggtcgcccc
     9181 actgctggac aatctcacct cggcgctgaa caaagtgtat caacgcaaag gggtcaatat
     9241 ctctctcgat atttcgccag agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt
     9301 gatgggcaac gtgctggata atgcctgtaa atattgcctc gagtttgtcg aaatatctgc
     9361 aaggcaaaca gacgagcatc tctatattgt ggtcgaggat gatggccccg gtattccatt
     9421 aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt gatactttac gccctgggca
     9481 aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc
     9541 cggagagagc atgctgggcg gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc
     9601 gccgaaagat gaataaatat gtccatactt cacgcattac gttaagcatc cgttataatc
     9661 ggttgcagat accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc
     9721 cgattttctt gaacgtcact ggcagaaacg tccggtggtg ttaaaacgcg gctttaataa
     9781 ttttattgac ccgatctctc cagacgagtt ggcgggtttg gcgatggaaa gcgaagtcga
     9841 cagtcgactg gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag
     9901 ctacgatcat ctcggtgaaa ctaactggtc attgttagtg caggcagtga accactggca
     9961 tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga
    10021 tctgatgatc tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga
    10081 cgtgtttatc attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat
    10141 gaaacagcac tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga
    10201 tgaagagctg gagcctggcg atatccttta tattccgcca ggattcccgc atgaaggcta
    10261 cgcgctggaa aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt
    10321 aattagcgga tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga
    10381 tccggatgtt ccacctcgcg ctcatcctgc ggacgttctg ccgcaagaga tggataaact
    10441 gcgtgagatg atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga
    10501 gtttatatcc cagtcacgtc atgaactgga tatcgcgccg ccagagccgc cttatcagcc
    10561 ggatgaaatc tacgatgcgc taaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg
    10621 cgtattgcgc attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg
    10681 tccggcactg gatgcgctcg ccagcaacat tgcgctgacc gcggaaaatt ttggtgatgc
    10741 actggaagat ccgtcattcc tcgcgatgct cgcagcgctg gtcaatagcg ggtattggtt
    10801 cttcgaaggg taagtttgaa gttaagctgg atgcggcatc acatgccgca tcccttttcg
    10861 cttacttccg ttgcgccgtc aactcggcaa tacggacgat cacctgcacc gctttttcca
    10921 taccttccag agtcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt
    10981 tcgggcacgg taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt
    11041 tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg
    11101 gatgctcaac cactttctcg cgcatattgt agtaactgtc ttcaatcacc aattcaatgt
    11161 agcaatcagg atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac
    11221 gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa
    11281 cggtgccttt catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc
    11341 tttcatccgc cggaacttcc gcatgaatac gtgccgccag cgacagcgca ttcaccatta
    11401 cacctttcgc tgtgcctggg tgaacgttat tgcctacaat tttgatattg accgacgcgg
    11461 cgttgaagtt ttcaaactcc agttcgccta cgccaccacc atcaacggtg taagcccagc
    11521 gggcatcgaa ggcgtcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg
    11581 taaaggcgac gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg
    11641 tcatgatttc tgcaatacct gctttgtcat cggcacctaa caaggtttta ccatcggtgg
    11701 taatcagcgt ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt
    11761 catcgccgat acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattca
    11821 catttttgcc gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga
    11881 tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca
    11941 cattgataag ccccatctct tcgagctgct ctttcagcag atgcaataac ttccattggc
    12001 cttccgtgct gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt
    12061 tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat
    12121 taagaagcaa gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata
    12181 tttatcagca ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc
    12241 gattcaagcg gtttgccgta gaattaccgc cctcattaag agtcaccaag gtggttaacc
    12301 acaaaccccg catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa
    12361 taaacaaccg agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga
    12421 tggtaaagag gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct
    12481 gcttggccct tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac
    12541 tgttgattcc ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa
    12601 ccgctatgtg aatactgttt tccaaagcta cgcacttttc ccccacatga ccgtgttcga
    12661 aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt
    12721 gatggaagcc ctgcggatgg tgcagctgga aaccttcgct caacgcaaac cgcatcagct
    12781 ctctggtggt caacagcaac gcgtcgccat tgcccgcgcg gtggttaaca agcctcgtct
    12841 gttgttgctg gatgagtcgc tctcagcact ggattacaaa ctgcgcaagc aaatgcagaa
    12901 cgagctgaaa gcgttacagc gtaagcttgg cattacattc gtcttcgtga ctcacgacca
    12961 ggaagaagca ctcaccatgt cggacaggat tgtggtgatg cgcgatggtc gcattgagca
    13021 agacggcacg ccgcgtgaaa tctatgaaga gccgaaaaac ctgtttgttg ctgggtttat
    13081 tggcgaaatc aatatgttta acgccactgt catcgaacgt ctcgacgagc agcgcgtacg
    13141 cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca
    13201 aaaactgcat gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa
    13261 ccacgctgaa gggctgattg gttacgttcg cgagcgtaac tacaaaggca tgacgctgga
    13321 gtcggttgtt gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga
    13381 cgatcctgac tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg
    13441 ggaggtcgta ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta
    13501 ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct
    13561 ttttgacccg cgacgacgcc agtttcgtca aaatggtctt tacgctggat aactacacgc
    13621 gtctgctcga tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg
    13681 ccaccctcgc ctgcctggtg ctgggctatc cttttgcctg gtttctggcg aagctgccac
    13741 acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc gttctggacc aactcattga
    13801 ttcgtatcta cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc
    13861 tctggctggg cgttattgat acaccaattc gcatcatgtt cacgcccagt gcggtgatta
    13921 tcggtctggt ttacattctg ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa
    13981 aactggataa gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttgcagacct
    14041 ttatccgcat cattatccca ctgacgatgc cgggaattat tgccggatgt ctgctggtga
    14101 tgctgccagc gatgggcctg ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc
    14161 tgatcggtaa cgtcatcaag gttcagttcc tcaatattcg tgactggccg tttggtgcag
    14221 ccaccagcat tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt
    14281 ctcgtctgct gaacaaaaag gtggaactcg aatgatcggt cgactgcttc gcggcggttt
    14341 tatgaccgct atctacgcgt acctgtatat cccaatcatt attttgattg tgaactcctt
    14401 taacagctcg cgctttggca tcaactggca gggttttacc accaaatggt atagcctgct
    14461 gatgaacaac gacagcctgt tacaggcagc gcagcattca ctaacaatgg cggtgttttc
    14521 ggcgacgttt gctacgctta tcggttcact gacggcagtt gcgctgtacc gttatcgctt
    14581 tcgtggtaag ccgttcgtta gcggaatgct gtttgtggtg atgatgtcac cagatatcgt
    14641 gatggcgatt tcgctgctgg tgctgtttat gctgctgggt attcagcttg gcttctggtc
    14701 gctgctgttc tcgcatatca ccttctgcct gccatttgtg gtggtgacgg tgtactcgcg
    14761 cctgaaaggt tttgacgtgc gaatgctgga agcggcgaaa gatctcggtg ccagcgaatt
    14821 taccattctg cgaaaaatca ttctgccact ggcaatgcca gcggtggcgg cgggctgggt
    14881 gttaagcttt accctgtcga tggacgatgt ggtggtttct tcgttcgtca ccggaccaag
    14941 ttatgaaatt ctgccgttaa aaatttattc gatggttaaa gtcggcgtat cgccggaagt
    15001 taacgcgctg gcaaccatat tactggtgct gtcgctggtg atggtaattg ccagccagct
    15061 tattgctcgt gataaaacga aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc
    15121 cacctgctcg cggcgggtgc tctggcactg ggcatgagcg ccgctcacgc cgatgacaac
    15181 aacacgctgt atttctacaa ctggaccgag tacgtgccgc caggactgct tgaacagttc
    15241 accaaagaaa ccggtattaa ggttatctat tcgacttacg agtcgaacga aaccatgtac
    15301 gcgaagctga aaacgtacaa agacggtgcc tatgacctgg tggttccttc aacctattac
    15361 gtcgataaaa tgcgtaaaga agggatgatc cagaagatcg acaagtcgaa gttaagcaac
    15421 ttcagcaacc tcgatccaga catgctcaac aagccgtttg acccgaataa cgactactcc
    15481 attccgtata tctggggggc gacggcgatt ggcgttaacg gtgatgcggt agatccgaaa
    15541 tccgttacca gctgggccga tctgtggaag cctgagtaca aaggcagcct gctgctgacc
    15601 gacgatgccc gtgaagtgtt ccagatggcg ctgcgcaaac tgggctactc cggtaacact
    15661 accgatccga aagagattga agctgcatat aacgagttga aaaaactgat gccaaacgtc
    15721 gcggcgttta actccgataa ccccgctaac ccgtacatgg aaggcgaagt taacctcggc
    15781 atgatctgga acggttctgc tttcgttgca cgccaggcgg gtacgccaat tgatgtggtg
    15841 tggccgaaag aaggcggcat tttctggatg gacagcttgg cgatcccggc aaatgccaaa
    15901 aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc gcccagatgt ggcaaaacag
    15961 gttgcagaaa ctatcggtta tccaacgcca aaccttgcgg cgcgtaagct gttaagtccg
    16021 gaagtggcga acgataaaac gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg
    16081 cagaatgacg ttggcgcagc cagcagcatt tatgaagagt attatcagaa gctgaaagca
    16141 ggacgttaat tcagcaagcg ggcggtggta ttaccgcccg ttttttattt agcaaggatg
    16201 tggctaatga gtcaggacag tagagttttc tttcatatat tcctcggtat cggtctggta
    16261 ctaatcctca tttctgttgt tgtcttttat aaccagttca cctatagcaa agatgcgatt
    16321 catactgaag gcgtcattgt tgataccgtc tggcactcca gtcattctca tcgcacgggt
    16381 aaaaatggtt cgtggtatcc cgtcgttgcc tttcgaccga cgcctgatta cacgctaatt
    16441 ttcaattcga gtattggcag cgatttttat gaagacagcg aaggcgataa agttaatgtt
    16501 tattattctc caggacatcc tgaaaaggct gagataaata acccgtgggt taattttttt
    16561 aaatgggggt ttatcggcat tatgggcgtt atttttatct ccgtcggttt attaatcagt
    16621 atgccctcct ctaaaaagac acgcaggaaa cggaagtccc gcccatgaaa aaaatcataa
    16681 tattgctcag tttactgata ctcctgcctc tgacggcaac cagtaatcca ttgattccaa
    16741 taatgaagac tctttttaca gatgttacag gtactgtacc ggatgctgaa gagatcgcgc
    16801 ataaagccga gctttttcgc caacaaaccg ggattgcgcc gtttatcgtg gttgtacctg
    16861 gcgttaatga agcaagcttc aggcaaaatg gcaaagcgat gctggcgcat gcgtcatctt
    16921 cattgagtga tgtaaaaggc agcgtcctgt tactttttac cacccgcgag ccacggttaa
    16981 ttatgataac caacggccag gttgaaagcg gtctggacga taaacatctc ggccttctgg
    17041 tagaaaatca cactcaggct tatttacatg cggacctctg gtatcaggga atcaataatg
    17101 cattggctgt tttacaagca cagatattaa aacaaccgac gccgccactg acatattatc
    17161 cgcatcccgg acaacagcag gagaatgatc ctcccggcag taccacgata ttaggtcttt
    17221 ttgcctgggc agttgcattc atcgtcttta ctgcattttt ttactacacg acgcgcctta
    17281 attatgcatt aaagtttgca gtggcgatga ctattgccaa tatgggttat caggctttat
    17341 gtttgtacat cgataatagc ttggcgatta ccagaatatc gccgctctgg gcagggctga
    17401 ttggtgtctg tacgtttatt gccgcgttgt tattgacgag taaacggtaa aagaggcagt
    17461 aaagtgtgcg tttattatgc cggatgcggc gcgaacgcct tatctggccc acaaaacccg
    17521 caaattcaat taattgcgtc cccttgtagg cctgataagc gtagcgcatc aggcaatgct
    17581 tcctgcttta taagcccttc agcaactttt caacaaactc tggcaccacc tggcttgccg
    17641 ggccgtaata tttctcggca aattcattac cgacctggct tggttcaaga ttcagttcca
    17701 cggtgtgtgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc
    17761 ctgatgtacc aatggcaatg aaaatatcgg ccattgacaa cgccatataa atttcatcca
    17821 tgccgagtgg catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc
    17881 aacaatggca tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt
    17941 gtgaacaacg aactttcagc agttccccat gcatatgaat aacattggtg ttaccggcac
    18001 gctcatgaag gttgtcgata ttctgcgtca ccagcaaaaa gcgatcgccg agggcatctt
    18061 gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgtagctgtc
    18121 gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg
    18181 ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa
    18241 tacctgattc cgcagaaata cctgcccctg tcagtacgag tactcttggt ttttccattg
    18301 cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcagacggc
    18361 gtttattttt acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg
    18421 ttgtttaatc ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc
    18481 gcgcgcgttc aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctgtccg
    18541 ccagttgcgt tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag
    18601 ggtcaacaat ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa
    18661 cgtgcgccct tgcctgctca tcaccttgat cataaagcgc aataatttcg ggagcctgca
    18721 acggttgatg ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt
    18781 caatgcagcc atgctgacca cagccgcagc ggcgtaacgg gaaatccagc cccatcatgg
    18841 ttaacgcatc aaccggcaga cgcatatggc caaactcgcc ggtaatatag cttttaccgg
    18901 taattggttt accgttgaaa atcagcccgc cgccaacgcc ggtgccgaga atcaacccca
    18961 tcaccagtgg atattgagta aattcatcat cccaggcttc tgaaagggca aaacagttgg
    19021 catcgttatc aaggcggaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt
    19081 taccgctagc agcggggaca ttggcagcat acagcgtacc atcttctgtt tccggcattc
    19141 ccggaatacc gatgccgaca gaacctttac agccaaaacg cttatcggct tcagccacca
    19201 gctcgcacac tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt
    19261 cccactgcaa ctgccgaacg ctatcaaaca cgccaagcgc aattttcgtt ccaccaatat
    19321 caaacccgta atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg
    19381 actcgcgcag ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc
    19441 agcaacaatg ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg
    19501 aagtcaataa aatagatatc gctggagagg aactgatgac cgatcaactt ttcaatccac
    19561 tcaataatcg gggtaagttg cagtgaaaca actacgccga taatcacacc acacaggcta
    19621 ccgaatagcc ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc
    19681 gcccccagcg ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag
    19741 gtggagacga tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg
    19801 gcgcggatca tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata
    19861 taaacatagc tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca
    19921 tccgtcattt taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca
    19981 tctgccagcg ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg
    20041 gcaatgtgca aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg
    20101 atcataatcg acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg
    20161 atgataattt gctgttcgcc cgctttaaaa ttgcgccagg catccccctg aacaaacgag
    20221 ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc cctttacctg gattgcgcga
    20281 agattcgcgc cactttctac cagcccggtg aaattgatat acggcgcagc ggcggcaata
    20341 cccggcactt tctgcacttt atccagtgct tcctgccagt tagtccacgg ctgatccacc
    20401 gcttcgattt caccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag
    20461 ccgttcatcg cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta
    20521 gaaatgacgg agatcagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg
    20581 ccaattaata acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg
    20641 tcagacgacc atcacgcatc tccagttggc ggctcatacg tttcgccagt tgcaggtcgt
    20701 gagtaaccac caggaaggcg gtgccctgca aacgattcaa ctccccaagt aactggaaga
    20761 tgctgtcggc gttacgcgca tcaaggttac cggtaggttc atccgccagt accagacgcg
    20821 ggttattgac cagcgcacgg gcaatcgcca cacgctggcg ttcgccacca gaaagttcag
    20881 atgggcggtg attcgcacga tgctccagcc ccaccgcttt taacatctcc agcgcacggc
    20941 tgttgatttc ggcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg
    21001 cagtaaaatc cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca
    21061 gttctgcttt cgccgctgac gacagtttgc tcatcggctg accgttaaag atcacatcgc
    21121 cggaggttgg cgtatccagc ccgcccagca ggtgcagcaa ggtgctttta ccggaaccag
    21181 agctaccgac gatcgccatc atttcacctt cgccgacgct gaaactgaca ttgtgcagta
    21241 catcagtttg cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga
    21301 tcttattcat aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa
    21361 agcgtagaca gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt
    21421 tcgatagcga ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta
    21481 tttaactggc tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg
    21541 gcgctggccc cttgcaccat aaagaccatc atgatttgcc gcggagtcag cccctgagtc
    21601 tgaagaatgg ccacttcgcc ctgcttctcc atcaccatta accccagcga ggtaataatg
    21661 ttaaacgccg caacggcgac aatcaggctc agcagcaagc ccatcatatt tttttccatg
    21721 cgtacggcct ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca
    21781 ggcagttttt gttgacttaa tgagtcgact ttcagcggct catccagcca caatcgccag
    21841 ccggtaatat tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc
    21901 atttcatagc catcgacttc actgttggca gcgaaagtgc caatcacatt gaacagacgc
    21961 tggcttggaa tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt
    22021 tgatcaccgc gattgacgcc caactgtgag gcaagctgtt caccgaggat gacattatat
    22081 ttccccggct cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt
    22141 tgcgccggat cgatacccag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc
    22201 acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc
    22261 gggagttgct gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg
    22321 ccaaggatgt tgttttgcag ctcgcgctca aaaccgttca tcactgacaa tactgtgacc
    22381 agcgccatca ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag
    22441 cgatccgctg cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac
    22501 atgaaatccg tctggttgct gtagcaaaga cacgagtata taagcgaaag cgggatgggt
    22561 aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa aagtagcagc aaatcaatcg
    22621 actaaagggc ctcattttct cattggcctt gtggcctgat ctttatctga aaaataacca
    22681 aatacagaca gatacgcatt acaccacgcg actggcccct caggatcaac agcatgttaa
    22741 ataaaaagag cgtgttgtcc tgatgaaaca aaaagagcta tggattaacc agatcaaagg
    22801 attatgtatt tgtctggtag tgatttatca ctcggtgatt accttttatc cgcatctgag
    22861 cacttttcag catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta
    22921 ccttgcaccc tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta
    22981 tatcgacagc gtaccgtggg gaaattgtct cgataaacgg atctggaaca tcttctgggt
    23041 gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc
    23101 acctgagcgc gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt
    23161 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    23221 tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact
    23281 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg
    23341 caatttgcct tattacagcc ttggcgcatg gtttggcgca acattaatga cctacgttaa
    23401 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg ctgaccgttc tggcggtcgg
    23461 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    23521 gctgttttat caatacgagc aacgtttcgg tatgcgctcc accagcctgc tgaatgtgat
    23581 tggcaccaac accattgcta tctacaccac ccatcgcatt ctggtagaga tattcagctt
    23641 aactctgctt gcgccaatga acgcggcacg ctggtcgccg caagtcgaac tgacactcct
    23701 gatggtttac ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    23761 actttcacag cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    23821 cagttactcc cgctaagcca tcgccccagc cggggcgatt caatttgcta aaccatgtcg
    23881 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    23941 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    24001 cgggtgagca gcgtctgctg ggcgagttaa ctggcgctgc ctgcgcgacg ctggtagcgg
    24061 aaattgccga acgtcacgcc ggaccagtgg tgcttattgc gccagatatg caaaatgctc
    24121 tgcgtttgca tgatgaaatt agccagttta ccgaccagat ggtgatgaat ctggcggact
    24181 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    24241 ccacccttta ccagctaccg acgatgcaac gtggtgtact gattgttccg gtgaatacgc
    24301 ttatgcagcg cgtttgcccg cacagttttc tccacggcca tgcgctggtg atgaaaaaag
    24361 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgccatg
    24421 ttgaccaggt tatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc
    24481 cgatgggaag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagtctgc
    24541 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc
    24601 ccgcgcacga atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    24661 ataccttcga agtgaagcgt gatccggaac atatttacca gcaagtgagt aaaggcacat
    24721 tacctgcggg gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt
    24781 tcagttattt ccctgccaat accttgctgg tgaatactgg cgatctggaa aacagtgccg
    24841 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc
    24901 cactgttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    24961 actggccccg agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt
    25021 taggtttcca gaaactgcca gacctggccg ttcaggcaca acaaaaagcg ccgctggatg
    25081 cgctgcgtaa gttcctcgag tctttcgacg gtccggtggt gttctcggta gaaagtgaag
    25141 gtcgccgtga agcgctgggt gaactgctcg cgcgaattaa aattgctccg caacgcatta
    25201 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    25261 gttttgtcga tacggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac
    25321 gcgttgcccg tcgtcgtcag gattctcgcc gcgccatcaa ccccgataca ctgatccgta
    25381 acctcgcgga gctgcatatt ggtcagccgg tggtccatct ggagcacggt gtcggtcgtt
    25441 atgcaggaat gaccacgctg gaagctggag gcattactgg cgagtacctg atgctcacct
    25501 atgccaacga cgccaaactg tatgttccgg tatcgtcact gcatctgatt agccgttacg
    25561 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg
    25621 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc agaattactg gatatttacg
    25681 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgcgagcag tatcagttgt
    25741 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc acaggccatt aatgcggtac
    25801 ttagcgacat gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    25861 gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtgg
    25921 cggtgctggt gcctaccacc cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt
    25981 tcgccaactg gccggtacgt atcgaaatga tctcccgttt ccgcagcgtc aaagagcaga
    26041 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    26101 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    26161 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg tgcgaacgtg gatattctga
    26221 cgcttaccgc tacgccgatc ccacgtacgc tgaatatggc gatgagcgga atgcgcgatc
    26281 tgtcgattat tgccacgccg cccgcccgtc gtctggcggt caaaacgttt gtccgcgagt
    26341 atgacagtct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    26401 attatctcta caatgatgtg gaaaacatcc agaaagccgc cgaacggctg gcagaactgg
    26461 tacctgaagc gcggattgcc atcggtcacg ggcagatgcg tgagcgcgaa ctggaacggg
    26521 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    26581 ccgggatcga catcccgaca gccaacacca ttatcattga acgcgcggat cacttcggtc
    26641 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat
    26701 ggttgctgac accgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    26761 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    26821 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    26881 cgctgtatat ggagttgctg gaaaacgccg tcgatgcgct gaaagccgga cgcgagccgt
    26941 cgctggaaga tctcaccagt caacaaacag aagtcgagct gcggatgccg tcgctgttgc
    27001 cggatgattt cattcctgac gtgaatacgc gtttgtcgtt ctataaacgt attgccagcg
    27061 ccaaaacgga aaacgaactg gaagagatca aagttgagct tatcgatcgc ttcggcctgc
    27121 tgccggatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    27181 tggggatcag gaagctggaa ggtaatgaga aaggcggcgt gatcgaattt gccgagaaga
    27241 atcacgttaa tccggcatgg ctgattggtt tgctgcaaaa acagccgcag cattatcgcc
    27301 tcgatggccc gactcgcctg aagtttattc aggatttgag cgagcgaaaa acgcgtatcg
    27361 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tagtaaatcg
    27421 ggggcgtttc tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat
    27481 ccgacactgt caccatccat aattcagact gatcttttta ttacaataac cttatattta
    27541 ttatggattt ttggtgatga tcaaaacgca tttttctcgc tggctaacgt tttttacgtt
    27601 cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac acctggccac tgccgccagc
    27661 gggcagtcgt ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct
    27721 ggaagccatc gccaaaaaat ataatgtcgg ctttctcgct ctgttacagg ctaaccccgg
    27781 cgttgatcct tacgtgccgc gcgcgggtag cgtgttaacg atcccgttgc aaaccctact
    27841 tccagatgcg ccgcgcgaag gcattgtgat caatattgcg gaactgcgtc tctattacta
    27901 cccgccgggt aaaaattcgg taaccgtgta tcccatcggc attggtcagt tgggtggtga
    27961 cacgctgacg ccaacgatgg tgactaccgt ttcggacaag cgtgcaaacc caacctggac
    28021 gccaacagcg aatattcgcg cccgttataa agctcagggg attgagctac ctgcagtggt
    28081 tccggcagga ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg
    28141 tgtttatttg cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg
    28201 ctgtattcgt ctgcgtgatg acgatatcaa aacactcttt agccaggtca cccctggtac
    28261 caaagtgaat atcatcaaca ccccgattaa agtctctgcc gaaccaaacg gtgcgcgtct
    28321 ggttgaagta catcaaccgc tgtcagagaa gattgatgac gatccgcaac tgctgccaat
    28381 tacactgaat agcgcaatgc aatcatttaa agatgcagcg caaactgacg ctgaagtgat
    28441 gcagcatgtg atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag
    28501 cccacaaact ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga
    28561 aaaaatcagc agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt
    28621 ccatggaggg tatttggacc ggttacagaa gtaatacgga atgattttgc gcccatctca
    28681 tccgcttttt gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg
    28741 atagtaccga ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagctg
    28801 gcaaatgaca ttgagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata
    28861 gtggccttat gcagatgaat gacgtcgcaa gcaattactt
//
LOCUS       DARNVM010000047        28590 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.047, whole genome shotgun sequence.
ACCESSION   DARNVM010000047 DARNVM010000000
VERSION     DARNVM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.047"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..593)
                     /locus_tag="SI621_001841"
     CDS             complement(<1..593)
                     /locus_tag="SI621_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEI4064568.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="SI621_001842"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="SI621_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEI4064569.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="SI621_001843"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="SI621_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEI4064570.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="SI621_001844"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="SI621_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEI4064571.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="SI621_001845"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="SI621_001845"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEI4064572.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="SI621_001846"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="SI621_001846"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEI4064573.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPLTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="SI621_001847"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="SI621_001847"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEI4064574.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSCVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /locus_tag="SI621_001848"
     CDS             complement(4982..5704)
                     /locus_tag="SI621_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEI4064575.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="SI621_001849"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="SI621_001849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEI4064576.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /locus_tag="SI621_001850"
     CDS             6904..7122
                     /locus_tag="SI621_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEI4064577.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7172..7762)
                     /gene="slyD"
                     /locus_tag="SI621_001851"
     CDS             complement(7172..7762)
                     /gene="slyD"
                     /locus_tag="SI621_001851"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEI4064578.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7857..8057)
                     /locus_tag="SI621_001852"
     CDS             complement(7857..8057)
                     /locus_tag="SI621_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HEI4064579.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8067..9872)
                     /gene="kefB"
                     /locus_tag="SI621_001853"
     CDS             complement(8067..9872)
                     /gene="kefB"
                     /locus_tag="SI621_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HEI4064580.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGAADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVLSVIVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9872..10423)
                     /gene="kefG"
                     /locus_tag="SI621_001854"
     CDS             complement(9872..10423)
                     /gene="kefG"
                     /locus_tag="SI621_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HEI4064581.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10554..12473
                     /locus_tag="SI621_001855"
     CDS             10554..12473
                     /locus_tag="SI621_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4064582.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDHKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKEKENANSAQARKDQKRREAELRAQTQPLRKEI
                     ARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLE
                     AQEQLEQMLLEGQSN"
     gene            12473..13495
                     /locus_tag="SI621_001856"
     CDS             12473..13495
                     /locus_tag="SI621_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEI4064583.1"
                     /translation="MAQITTTNANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQRESGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13489..13707
                     /locus_tag="SI621_001857"
     CDS             13489..13707
                     /locus_tag="SI621_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEI4064584.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13761..14630
                     /locus_tag="SI621_001858"
     CDS             13761..14630
                     /locus_tag="SI621_001858"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEI4064585.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14685..15089)
                     /locus_tag="SI621_001859"
     CDS             complement(14685..15089)
                     /locus_tag="SI621_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEI4064586.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15391..16023
                     /gene="crp"
                     /locus_tag="SI621_001860"
     CDS             15391..16023
                     /gene="crp"
                     /locus_tag="SI621_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEI4064587.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16074..18164
                     /locus_tag="SI621_001861"
     CDS             16074..18164
                     /locus_tag="SI621_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEI4064588.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19450)
                     /gene="argD/dapC"
                     /locus_tag="SI621_001862"
     CDS             complement(18227..19450)
                     /gene="argD/dapC"
                     /locus_tag="SI621_001862"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HEI4064589.1"
                     /translation="MATEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGGK"
     gene            complement(19536..20099)
                     /gene="pabA"
                     /locus_tag="SI621_001863"
     CDS             complement(19536..20099)
                     /gene="pabA"
                     /locus_tag="SI621_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HEI4064590.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPVCFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20131..20733)
                     /locus_tag="SI621_001864"
     CDS             complement(20131..20733)
                     /locus_tag="SI621_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HEI4064591.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKEKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20723..20890)
                     /locus_tag="SI621_001865"
     CDS             complement(20723..20890)
                     /locus_tag="SI621_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HEI4064592.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20995..21567)
                     /gene="ppiA"
                     /locus_tag="SI621_001866"
                     /gene_synonym="rotA"
     CDS             complement(20995..21567)
                     /gene="ppiA"
                     /locus_tag="SI621_001866"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HEI4064593.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21838..23019
                     /gene="tsgA"
                     /locus_tag="SI621_001867"
     CDS             21838..23019
                     /gene="tsgA"
                     /locus_tag="SI621_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HEI4064594.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23281..25824
                     /gene="nirB"
                     /locus_tag="SI621_001868"
     CDS             23281..25824
                     /gene="nirB"
                     /locus_tag="SI621_001868"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HEI4064595.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIKTHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25821..26147
                     /gene="nirD"
                     /locus_tag="SI621_001869"
     CDS             25821..26147
                     /gene="nirD"
                     /locus_tag="SI621_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEI4064596.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26273..27079
                     /gene="nirC"
                     /locus_tag="SI621_001870"
     CDS             26273..27079
                     /gene="nirC"
                     /locus_tag="SI621_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HEI4064597.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27098..28471
                     /gene="cobA"
                     /locus_tag="SI621_001871"
     CDS             27098..28471
                     /gene="cobA"
                     /locus_tag="SI621_001871"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEI4064598.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6904 a         7687 c         7233 g         6766 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaaatc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcatattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggagcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtatct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     2041 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcgtga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag tcaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataacagca gttcgtcgcc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aatctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtcagcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacacc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggtgcagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccga ctgaagattt
     4681 gacgaagcca gccccagcct tcctgcttca gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagccgcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgatagaaaa agacgcagct taactcatgg ccttcggcta tcagtgcttg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcgtcttta atatcgaaga
     5101 tccctttctc gtagagattt agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagagtt ttccatgtaa cgacccagcg aggcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat tggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attcccccgc ttttttatca ctttttcggt attagtggca accgcaaccg ccgttacctt
     7201 taccgccgca gcagccttcg ccaccgtgtt catgaccgtg atcgtggcca tgaccaccgc
     7261 agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc accgtgaacg tggccatgag
     7321 ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc aacgttgaat ttcaggttct
     7381 gaccggccag catgtggtta ccatcaacca cgacgtgatc gtcttcaacc gcagtgattt
     7441 caaccggtac cggaccctgg tcggtttcag ccaggaaacg catacctacc tgcagttcat
     7501 caacgcccat aaatacgtct ttaggaacgc gttgcaccag gttttcgtcg tactgaccgt
     7561 aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc aacttcgtga ccttccagcg
     7621 ccgtttccag gccagagatc agggaaccgt gaccatgcag gtagtccagc ggcgcactca
     7681 ccggagactc atcaaccaac acaccgtctt ctgtacgtac ctgataggcc aggctgacca
     7741 ccaggtcttt tgctactttc atgatatctc ctgagcatgg gaagaatagt ggcgcagatt
     7801 gtagcggaat tctgcagccg tgtactcact agcttaaaaa aactcggcgc atatcgctag
     7861 tccggatgaa aaatcccgat cacttgctca tctttgcgaa cgtgatcgcg ggcttctttg
     7921 tccgcttctc gcatctgatg tccgcactta acacattcaa caatatcaat attattttcg
     7981 cgccacatcg ccattgaatc ctgcgcctga caggccgggc attttgcgcc cgcaataaaa
     8041 cgttttcgga ttgccatctt taccctctac tcaaattcat cccagccgtc cagctggcgt
     8101 cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt cgcgtcgggc ttccctggcg
     8161 cgagaaattt gtacggtatc ggtatgcatt gggatgagct ctcgcagcat ccgcatatcc
     8221 aggcgacgaa aatgcagttg cgcccgctgt gcctgatgcg gatgcatgcc aagcgtgacc
     8281 agcgtcttgc gccccagctc taacgcactg gagaatgttt cacgggaaaa ctgcgtcacc
     8341 cctgcctgta ataactcatg cgcttccaca cgtccgcgcg ctcgcgcaag aatatgcaaa
     8401 tgcggaaagt gctgctggca tatttccacc agcttcatgg tgtcttccgg ctcgttacag
     8461 gtaatgacga tagactcagc ggcctctgct cccgcagaac gtaaaagatc gacctgcgtg
     8521 gcgtcgccgt aataaacttt gtagccatat ttacgcatca ggttaacggc actgatatcc
     8581 cgctccagca cagtaatgcg cattttattt gccatcagca aacgaccaat cacctgacca
     8641 aaacgcccaa agcccacgac aatgacctgg ggtttatcat cgttgaccca cggtttttcg
     8701 tcttcttctt ccggtccgtt aaactggcgg gatagccatt tatccaccag cttcatcagc
     8761 agcggcgtgg tcatcatgga aagcgtcacc gtcaccagca acagcgccat ctggtcaccc
     8821 tggaataagc gttgtgaaga agcggtagaa aagagcacaa aggcaaactc accgccctga
     8881 ctcaacacgc cagcaaactg catccgctct gagctacgca cgccatacag tcgcgccagc
     8941 agatacagca cgagaatttt caccgccacc aacacaataa cgcttaacac cacccacaac
     9001 agatgggtat aaagcacacc gaggttgagc gacattccga ccgagataaa aaatagcccg
     9061 agcagcaacc ccttaaacgg gtcgatagct gtttccagtt cgtggcgata ttcgctttct
     9121 gccagcaaca cgcccgcaat aaacgtaccg agcgccatcg acagccccag cgcatccata
     9181 aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg tgaacacttc ccgcacacca
     9241 gaagctgcaa taaaacggaa taccggacgc agtaaataac gcccgccaat cagcatgccg
     9301 acaaacgcca gcaccttcat gccgattttc atccagtcga aatgttcgtc tgccgccccc
     9361 gccaacaacg gcactaacgc cagtgccggg atcaccgcca gatcctgaaa cagcagtacc
     9421 gagaaaccta actggccgga ttcgctgcga ttcatccctt tctcacgcat cagttgcaac
     9481 gccattgcgg ttgaggacat cgcaaggcca ataccaccga ccaccgccgc ctgccaggcg
     9541 aaatccgtca gcatcaataa tcccgccagc aacgccgcgc ttaacagcac ctgtgccgca
     9601 cctacgccaa aaatcgaacg ccgcaattgc caaagcttgg agggattcaa ctccaggccg
     9661 atgataaaca tcaggaacac cacgccgagt tccgaaaagt ggaggatctc atcgacgtcg
     9721 ctaataaaac ccagccccca cgggccaatt gcaatccctg ccagcaaata tcccaacaca
     9781 gcgccaatac ccagccgcga tgccagcggc accgcagcca ccgccgcgaa gagaaacaac
     9841 actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc tcctggagac agcggatttg
     9901 ccagccagtc accgtaggct ctggcgtgac tcgccagctc ctgtgcgctt tgccgtctcg
     9961 cccagtaaat aatgatggga ctcaaccaat gcatccggca catgcccgcc gctagttcaa
    10021 aggggcgcag aacatcgctc atcgggtagc gattcaaggc gtcataacgg taagcacttt
    10081 ccggctcgcc ggtggtaatc acgctacgcc agtactttcc cgccagttgg tttccccccg
    10141 gcccgctggc aaaaccacga cttaataccc ggtccagcca ctctttcagt agcgccgggc
    10201 agctataggt ataaagagga tgctgaaaga caatcacctc gtgctcgcgc agtaatgcct
    10261 gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc gtaaaggtcg tgcacggtaa
    10321 cattgctgag ctgcgtggcc ggtttaagca gtacccggtt tgccaccgag tcctgagatt
    10381 ccggatgggc atacagcagc aaaactttcg ctggctgaga catcatcccc ctcccggtat
    10441 ggtttttgtg tatagtcgct gttttgggct accattgcgc ccggtgcggc agctcgccca
    10501 tacattacat tatcataatg ataagttaac atagtctgaa catacggcac cttatgattg
    10561 ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct gctggataat gccaccgcca
    10621 ccatcaaccc cgggcagaaa gtcggcctgg tgggtaaaaa cggctgtggt aaatctaccc
    10681 tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg cagctacacc tttccgggaa
    10741 gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt accgcaagcg gcgctggaat
    10801 atgtcattga cggcgaccgt gaatatcgtc aactggaagc gcagctacac gacgccaacg
    10861 aacgtaacga cgggcacgcc atcgccacta ttcatggcaa gctggatgct atcgacgcat
    10921 ggagtattcg ctcccgtgcc gccagcctgc tgcacggcct cggtttcagc aatgaacaac
    10981 tggagcgccc ggtaagtgat ttctctggtg gctggcgtat gcgtcttaac cttgcccagg
    11041 cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc gactaaccac ctcgatctcg
    11101 atgccgttat ctggctggaa aaatggctga agagctatca gggcacgctg atcctgatct
    11161 ctcacgaccg cgacttcctc gatccgatcg ttgataaaat tattcatatc gaacaacaaa
    11221 gcatgttcga gtacaccggc aactacagtt cgtttgaagt gcagcgcgcc acccgtctgg
    11281 cgcagcaaca agcgatgtac gaaagccagc aggaacgcgt ggcgcatctg caaagttata
    11341 tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc ccagagccgc attaagatgc
    11401 ttgagcgtat ggagctgatt gcccccgcgc acgtcgacaa cccgttccgc tttagcttcc
    11461 gcgcgccgga aagcctgcca aatccgttac tgaagatgga aaaggtcagc gcaggctatg
    11521 gcgatcgcat tattctcgac tcgattaaac tgaatctggt ccccggctcg cgcattggtc
    11581 tgctaggccg caacggcgcg ggtaaatcga cattaatcaa actgttagcc ggtgaacttg
    11641 cgccagtcag cggtgaaatt ggtctggcga aagggatcaa gctcggctac ttcgcccagc
    11701 atcaacttga atacctgcgc gccgacgaat cgcctattca acatctggca cgtttagcgc
    11761 cgcaggaact ggagcaaaaa ctgcgtgact acctcggcgg ctttggtttc cagggcgata
    11821 aagtgacgga agaaacgcgc cgcttctccg gtggggaaaa agcccgcctg gtgctggcat
    11881 taattgtctg gcagcgtccg aatctgctgc tgctcgacga accgactaac caccttgacc
    11941 tcgacatgcg tcaggcactg actgaagcat taatcgagtt cgaaggcgcg ctggttgtcg
    12001 tctcgcacga ccgtcatttg ctgcgttcca ccactgacga tctctacctg gttcacgatc
    12061 ataaagtcga accgttcgac ggcgatctgg aagattatca acagtggttg agcgacgtac
    12121 aaaagcagga aaaccagacc gacgaagcgc caaaagagaa agagaacgcg aacagcgccc
    12181 aggcacgtaa agatcagaaa cgtcgggaag cggagctgcg tgcgcaaacc cagccactgc
    12241 gtaaagagat tgcccgtctg gaaaaagaga tggagaagct gaacgcacag ctggcgcagg
    12301 cggaagagaa actcggcgac agcgaactgt acgatcaaag ccgtaaagcg gagttgaccg
    12361 cctgcctgca acagcaagcc agcgccaaat ccggcctgga agagtgcgaa atggcgtggc
    12421 tggaagccca agagcagctt gagcagatgc tgctggaagg ccaaagcaac tgatggcgca
    12481 gataacgacg accaatgcca atgaattcag cagcagtgct gaattcaccc ctatgcgcgg
    12541 ctttagcaat tgtcatctac aaaccatgct gccgcgtctg tttcgtcgca aggtgaaatt
    12601 caccccgcac tggcagcgac tggagttgcc cgacggcgat tttgtcgatc tcgcgtggag
    12661 tgaagatcct gcacaggcgc ggcataaacc gcgtttagtc gtgtttcacg ggctcgaagg
    12721 cagtctcaat agcccttacg cccacggtct ggttgaggcg gcgcaaaagc gtggctggct
    12781 gggcgtggtg atgcattttc gcggatgcag cggtgaacca aaccgtatgc accgcattta
    12841 ccattcgggc gaaaccgaag acgccagttg gtttttacgc tggctgcaac gcgagtctgg
    12901 tcatgcgcca acggctgccg tcggctattc gctcggcggt aatatgctgg cctgtttgct
    12961 ggcaaaagaa ggcaatgatc tcccgattga tgcggcggtg attgtctctg cgccgtttat
    13021 gctggaagcc tgtagctatc atatggaaaa gggcttttcc cgcgtctatc agcgttactt
    13081 gctgaacctg ttaaaagcca atgccgcacg caagctggct gcctaccccg gaacgctgcc
    13141 gattaatctc gcgcagttaa aatcggtacg tcgcatccgt gaatttgacg atctaatcac
    13201 cgccagaatt cacggctacg ctgacgctat cgactattat cgtcagtgta gcgccatgcc
    13261 gatgctcaat cggatagcca aaccgacgct gattattcac gccaaagacg atccgtttat
    13321 ggatcatcag gtgatcccga aaccggaaag tctccccccg caggtggagt atcagctgac
    13381 tgaacatggc ggtcatgttg gctttattgg cggtacatta cttcatccgc aaatgtggct
    13441 ggagtcacgc attcctgact ggttaacaac gtatctggag gcgaaatcat gttgattccg
    13501 tggcaagacc tctcccccga aacgctggaa aatttaattg aaagctttgt gttacgtgaa
    13561 ggcaccgatt atggtgaaca tgagcgtaca cttgaacaga aagtcgccga cgtcaaacgc
    13621 cagctacagt gcggagaagc ggtgctggta tggtcggagc tgcacgaaac ggtcaatatc
    13681 atgccgcgca gtcagtttcg cgaataacct gcaaccgtcg gcggacgaga taataatgat
    13741 agctaagtca tggagttact atgtctgcca aacatccggt cattgcggta acaggatcca
    13801 gcggcgcggg gaccaccacc accagcctcg cgtttcgtaa aatattcgcg cagttaaatc
    13861 tgcatgcagc tgaggtggaa ggcgacagtt ttcaccgtta cacccgcccg gaaatggaca
    13921 tggcgatccg caaagcgcgc gacgccgggc ggcatatcag ctacttcggc cccgaagcga
    13981 acgactttgg cctgctggaa caaaccttca ttgaatatgg tcagagcggc aaagggaaat
    14041 ctcgcaaata tctgcatacc tacgatgaag ccgtaccgtg gaatcaggta ccggggacat
    14101 tcaccccctg gcaaccttta ccggaaccca cggatgtact gttttatgaa ggtttacacg
    14161 gcggcgtagt cacgccacag cataacgttg cgcagcatgt ggatttactg gttggcgtgg
    14221 tgcctatcgt taaccttgag tggattcaaa aactgatccg cgacaccagc gagcgcgggc
    14281 actcacgaga agcagtgatg gactcggtag tgcgttcaat ggaagactat atcaactaca
    14341 tcacaccgca gttttcccgc acccatctta acttccagcg cgttcccacc gtcgacacct
    14401 caaacccgtt cgcggcaaaa ggtatcccgt cgctggatga aagctttgtg gtgatccatt
    14461 ttcgtaatct ggaagggatc gattttccct ggctgctggc gatgttgcaa ggctcattca
    14521 tttcccacat caatacttta gtggtgcctg gcggcaaaat gggtctggca atggaattaa
    14581 ttatgctgcc gctggtgcaa cgattgatgg aagggaagaa aatcgagtaa ctctgctatt
    14641 acgccggata aaatactatc cggcttcaca acgggatagt taaatcacgc ggcaaccact
    14701 tcatacgagt gagtaatatt caccgctttt tccagcatca acgccactga acaatatttc
    14761 tcggcagaga gatctaccgc acgcgcaacc gctgcgtctt tcaggtcgcg accggtgacg
    14821 ataaaatgca gattaatgtg cgtaaacagg cgtggtgcct cttcgcggcg ttcagaggtc
    14881 aattttactt cacaatcgac cacatcctga cgcccttttt gcagaataga aaccacatcg
    14941 atggcactgc aaccacccgc cgccatcaac accatttcca tcggacttgg tgctttatcg
    15001 cctgagttgc cgtccattaa aatctggtga ccagaggcgg attcgcccag aaaagttaac
    15061 ccttcgaccc acttcactcg cgcttgcatt tttgctactc cactgcgtca attttcctga
    15121 cagagtacgc gtactaacca aatcgcgcaa cggaaggcga cctgggtcat gctgaagcga
    15181 gacaccagga gacacaaagc gaaagctatg ctaaaacagt caggatgcta cagtaataca
    15241 ttgatgtact gcatgtatgc aaaggacgtc acattaccgt gcagtacagt tgatagcccc
    15301 ttcccaggta gcgggaagca tatttcggca atccagagac agcggcgtta tctggctctg
    15361 gagaaagctt ataacagagg ataaccgcgc atggtgcttg gcaaaccgca aacagacccg
    15421 actctcgaat ggttcttgtc tcattgccac attcataagt acccatccaa gagcacgctt
    15481 attcaccagg gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc tgtggcagtg
    15541 ctgatcaaag acgaagaggg taaagaaatg atcctctcct atctgaatca gggtgatttt
    15601 attggcgaac tgggcctgtt tgaagagggc caggaacgta gcgcatgggt acgtgcgaaa
    15661 accgcctgtg aagtggctga aatttcgtac aaaaaatttc gccaattgat tcaggtaaac
    15721 ccggacattc tgatgcgtct gtctgcacag atggcgcgtc gtctgcaagt cacttcagag
    15781 aaagtgggca acctggcgtt cctcgatgtg acgggccgca ttgcacagac tctgctgaac
    15841 ctggcaaaac aaccggatgc tatgactcac ccggacggta tgcaaatcaa aattacccgt
    15901 caggaaattg gtcagattgt cggctgttct cgtgaaaccg tgggacgcat tctgaagatg
    15961 ctggaagatc agaacctgat ctccgcacac ggtaaaacca tcgtcgttta cggcactcgt
    16021 taatcccgtc ggagtggcgc gttacctggt agcgcgccat tttgtttccc ccgatgtggc
    16081 gcaggctgat ttatcacccc gatatcaact atgcacttcg acaaacgctg gtgctatgtt
    16141 tgcccgtggc cgttgggtta atgcttggcg aattacgatt cggtctgctc ttctccctcg
    16201 ttcctgcttg ttgcaatatt gcgggccttg atacgcctca taaacgtttt ttcaaacgct
    16261 taatcattgg tgcgtcgctg tttgccacct gtagcttgct gacacagcta ctactggcaa
    16321 aagatgttcc tctgcccttt ttgctgaccg gattaacgct ggtacttggc gtcactgctg
    16381 agctggggcc attgcacgca aaattgcttc ccgcatcgct gctcgccgcc atttttaccc
    16441 tcagtctggc gggatacatg ccggtctggg aaccgttgct catctatgcg ttgggcactc
    16501 tctggtacgg attgtttaac tggttttggt tctggatctg gcgcgaacaa ccgctgcgcg
    16561 agtcactaag tctgctgtac cgtgaactgg cagattattg tgaagccaaa tacagcctgc
    16621 ttacccagca caccgaccct gaaaaagcgc tgccgccgct gctggtgcgc cagcaaaaag
    16681 cggtcgatct aattacccag tgctatcagc aaatgcatat gctttccgcg caaaataata
    16741 ccgattacaa gcggatgctg cgtattttcc aggaggcgct ggatttgcag gaacatattt
    16801 cggtcagttt gcatcagccg gaagaggtgc aaaagctggt cgagcgtagc catgcggagg
    16861 aagttattcg ctggaatgcg caaaccgtcg ccgctcgcct gcgcgtgctg gctgatgaca
    16921 ttctttacca tcgcctgccg acgcgtttta cgatggaaaa gcaaattggc gcactggaaa
    16981 aaatcgcccg ccagcatccg gataatccgg ttgggcaatt ctgctactgg catttcagcc
    17041 gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc gcgtgactta ctggccgata
    17101 aacagcggcg aatgccatta cttccggcgc tgaaaagtta tctgtcacta aagtctccgg
    17161 cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt tgccagcctg atgggcaccg
    17221 cgctgcatct gccgaagtcg tactggatcc tgatgacggt attgctggtg acgcaaaatg
    17281 gctatggcgc aacccgtctg aggattgtga atcgctccgt gggaaccgtg gttgggttaa
    17341 tcattgcggg cgtggcgctg cactttaaaa ttcccgaagg ttacaccctg accttgatgc
    17401 tgattaccac cctcgccagc tacctgatat tgcgcaaaaa ctacggctgg gcgacggtcg
    17461 gttttaccat taccgcagtg tataccctgc aactgttgtg gctgaacggt gagcagtaca
    17521 tccttccacg tcttatcgat accattattg gttgtttaat tgctttcggc ggtactgtct
    17581 ggctgtggcc gcagtggcag agcgggttat tgcgtaagaa cgcccatgac gctttagaag
    17641 cctatcagga agcgattcgc ttgattctga gcgaggaccc gcaacctacg ccactggcct
    17701 ggcagcgaat gcgggtaaat caggcgcata acactctgta taactcattg aatcaggcga
    17761 tgcaggaacc ggcgtttaac agccattatc tggcagatat gaaactgtgg gtaacgcaca
    17821 gccagtttat tgttgagcat attaatgcca tgaccacgct ggcgcgggaa caccgggcat
    17881 tgccacctga actggcgcaa gaatatttac agtcttgtga aatcgccatt cagcgttgtc
    17941 agcagcgact ggagtatgac gaaccgggta gttctggcga tgccaatatc atggatgcgc
    18001 cggagatgca gccgcacgaa ggcgcggcag gtacgctgga gcagcattta cagcgggtta
    18061 ttggtcatct gaacaccatg cacaccattt cgtcgatggc atggcgtcag cgaccgcatc
    18121 acgggatttg gctgagtcgc aagttgcggg attcgaaggc gtaatgcagg ctaaaccatc
    18181 ggatacagcg ttcacgccgc atccgacatc tttggcctga tgcgtcttat ttacccccaa
    18241 tcaccttcgc caccgcgtgg gcgaaacgat gcatcccttc atcgatatcc gcatcttcca
    18301 ccaccagcga cggcgcaaaa cgcatcacat caggcccggc attcagcacc attacgccag
    18361 cctctgcgcc cgcatacagg aaatcacgcg cctgaccttt gtactgtggt ttcagctccg
    18421 cgccaattaa cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct
    18481 tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc acttcagggg
    18541 tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg
    18601 tggaaccgtg agaaccagga tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc
    18661 taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc gtcacaccgt
    18721 agtgcatgta agcaaacaaa tcgccagtcc gccccatccc gcactgcact tcatcaaaca
    18781 ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg
    18841 cagccgtcac gccgccctcg ccctggatcg gctcaaccac caccgcacag gtgtgatcat
    18901 ccatcaccgc tttcactgcg tggagatcgt taaagggaac gtggatgatg tctgccggtt
    18961 tcggcccaaa gccgtcggaa tattttggct gcccacccac cgaaacggta aacagcgagc
    19021 gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacgggcta tgacgcacac
    19081 aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa
    19141 acaccacgcg ttcggcaaac gttgcctcaa tcagtttacg accaagacgc agcgccggtt
    19201 cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcgttca
    19261 ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat
    19321 actccttgcc ttgctgatcc cagattcggc tcccctgacc ttttaccgga ataaactctg
    19381 ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt
    19441 cagttgccat ctcatgatca ccctgttaca cataaataaa tgtgaaatta taatcacaaa
    19501 atatgcataa aaaatcactt aatagcaatc agaaatcagc gatgcaggaa attagccagc
    19561 agttgatgtc cttgttcgct gagaatactt tctggatgga actgcacgcc ttccagatcc
    19621 cactggcgat ggcgaatacc cataatctcg cgggtttcgc tccaggccgt cacttcaaag
    19681 cacacaggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga
    19741 tttgccagcc ccttaaatac gcccacacca ttatgtgtaa tcggcgaggt tttgccgtgc
    19801 atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc ctggtgaccg
    19861 aggcaaacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc aagggagatc
    19921 ccggcttcat ctggcgtaca gggtccgggt gagatgacaa ttttttgtgg tttaagggcg
    19981 tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc ccccagttca
    20041 caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag caggatcatg
    20101 gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt cgcttaccac
    20161 tttgctaaat atcatctgca gtgcggtgag atcccccatt gccccactct gatttgcctg
    20221 attccaggcc tctttttcga taccctgcca gctcagttgg tagcccgcat gaatcgccag
    20281 ttgctcgaag aagatccgct gtgccagtcc gcttcccaca cggaagggat gcagcacgtt
    20341 gatttcacaa tagtaatgtg ccagccgctc gacgaacttc tctttctcca ggccaaccag
    20401 ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga tataggcaaa
    20461 gtggcagaac ggcgtatcac cctgataaat atcaacttca cgcaattgcc ccgcccagtc
    20521 gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag
    20581 cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag
    20641 ccgctgctgc tggcggatgt tcagccggtt acgcatgata tcaaggccag gataaagata
    20701 cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct tcaaggcgcg
    20761 ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac
    20821 tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga
    20881 gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa gcggcaaatc
    20941 tgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgagaa atcattacgg
    21001 caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt acggaccaac
    21061 gtcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca ccactttacc
    21121 aaataccgcg taaccgaaat cacgctgacc gtggtcaagg aaggcgttat cggcaacgtt
    21181 gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc
    21241 acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg gttttttctg
    21301 ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga cgcggtgaaa
    21361 ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca cagacactgg
    21421 cgctttttgt ttatccagtt ccagttcgat gttaccagct gaggttgtca acaatacgtg
    21481 cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcga aaacagcagc
    21541 catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc agaaaaaaag
    21601 caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc taattttacg
    21661 tatctgaaat atatgtaacc tgacgatcac tttttattct gtgataaaaa tcacctctta
    21721 aaatgcaatt tagcaaccga ttgcaataaa acatttaatc agatcacaaa atcacctaaa
    21781 atcgcccgct cgcggcatag cccgctatgc cattttattt ttgaacgcag gccaatcatg
    21841 actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc actgaccggt
    21901 gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt caatctgcct
    21961 gtttccagta tgagtaatac cttcaccttc ctcaacgccg gcattttaat ctctatcttc
    22021 ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt tggctttctc
    22081 ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct gttctcggcg
    22141 gcgatgttca ttctcggggt ggtcagcggc atcaccatgt cgattggtac attcctgata
    22201 acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttaccga ctccttcttc
    22261 agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg cagcattgag
    22321 tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat tctgaccttc
    22381 ggctgtgagt tcccggcact gggcaaacat gcaccaaaaa cggatgctcc ggtagagaaa
    22441 gaaaagtggg ggatcggcgt gctgtttctc tccattgcgg cactgtgcta catcctcggt
    22501 cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat gagcctgaat
    22561 gatgctggta cgctggtgag taacttctgg atgtcataca tggtcggcat gtgggcgttc
    22621 agctttattc ttcgcttctt tgatttgcaa cgtatcctga ccgtactggc gggtctggcc
    22681 gcgattctga tgtacgtctt taacaccgga acaccggcac atatggcgtg gtcaattctc
    22741 gccctgggct tcttctccag cgcgatctat accaccatca tcaccctggg ttcacagcag
    22801 accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcgggac catcggtact
    22861 atgttgacct ttgtggttac cggcccgatt gttgaacata gcggtccgca ggcggcactg
    22921 cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc
    22981 agccgtcacc gtcagcataa caccctgacc tctcattaat tgctcatgcc gggcggcgct
    23041 atcgtcgtcc ggccttttcc tctcttaccc tgctacgtgc atctatttct ataaacccgt
    23101 tcaatttgtc tgttttttgc acaaacatga aatgtcagac aattccgtga cttaagaaaa
    23161 tttatacaaa tcagcaatat accccttaag gagtatataa aggtgaattt gatttacatc
    23221 aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa
    23281 atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa
    23341 gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg
    23401 cgcatcgctt atgaccgcgt acacctctcg tcttacttct ctcaccacac cgccgaagag
    23461 ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct ggtcggcgaa
    23521 cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg gcgcaccgtt
    23581 ttttatgaca agctgatcat ggcgaccggt tcctacccgt ggatcccacc aatcaaaggt
    23641 tctgatactc aggactgctt tgtctatcgc accattgaag acctcaacgc cattgaatcc
    23701 tgtgcccgtc gcagcaaacg cggtgccgtt gttggtggcg gcctgttagg tctggaagcc
    23761 gctggcgcgc tgaaaaactt aggtattaaa acccacgtta tcgaatttgc ccctatgctg
    23821 atggcggaac agcttgatca gatgggtggc gagcagctgc gtcgcaaaat cgaaagtatg
    23881 ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg tgtcgaagcg
    23941 cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat cgtcttctct
    24001 accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt tgctccgcgt
    24061 ggaggtattg tcattaatga ttcctgccag acttccgatc cggatatcta cgccatcggt
    24121 gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta caaaatggcg
    24181 caggtcgccg ttgaccatat tctcggtagc gaaaacgcct ttgaaggtgc tgaccttagc
    24241 gccaagctga aactgctggg cgtagacgta ggcggtattg gtgatgcgca cggtcgcaca
    24301 cctggcgcac gtagctacgt ttacctcgac gaaagcaaag agatctacaa acgccttatt
    24361 gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac cagcgactac
    24421 ggcaacctgc tgcaactggt gctgaatgct atcgagctgc cggaaaaccc ggattctctg
    24481 attctgccgg cgcactctgg tagcggtaag ccgtctatcg gtgttgataa actgccggac
    24541 agcgcgcaaa tctgctcctg cttcgacgtc accaaaggcg acctgattgc tgctatcaac
    24601 aaaggctgtc acaccgttgc ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt
    24661 ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa
    24721 gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt ccatttaatc
    24781 cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga aacacggcaa aggctacggt
    24841 tgtgaagttt gtaaaccgac cgtcggttcg ctgctggcct cctgctggaa cgaatacatt
    24901 ctgaaaccgg aacatactcc gctgcaggat tctaacgata acttcctcgc taacatccag
    24961 aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg
    25021 ctgatggcag taggtcgtat cgcgcgtgaa tttaatctct acaccaaaat caccggctcc
    25081 cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc cggagatctg gcgtcagctg
    25141 attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc
    25201 tgcgtgggta gcacctggtg ccgctacggc gttggcgaca gcgtcggcct cggcgtggaa
    25261 ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc
    25321 tgtacccgtg aatgttcaga agcccagggt aaagacgtgg gtatcatcgc caccgaaaaa
    25381 ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg
    25441 gcggcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat gatgttctac
    25501 atccgtactg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct tgaaggcggc
    25561 atcgattacc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc acatctggaa
    25621 gaagagatgg cgcgcctgcg tgaagcggta gtatgtgagt ggactgagac ggtcaataca
    25681 ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga cccgaacgtg
    25741 cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccggta
    25801 actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc gatgacatcc
    25861 tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca ggtagcaatt ttccgcccgt
    25921 atcacagcga ccaggtgttt gcgatcagca acatcgaccc gttcttcgag tccagcgtgt
    25981 tgtcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa
    26041 aacagcgttt tcgcttaagc gacggcttgt gcatggaaga cgaacagttt tccgtcaagc
    26101 attacgaagc gcgagtgaaa gacggcgtgg tgcagctacg cggttaatgt tttaacggga
    26161 ggcgcaatgc ctcccctttt tgcatggtcc tgtaataacc ttcgatatat tgcaggacat
    26221 tttttaaact ttttgtttta ttttttgctt ttatttttta aaggataatc aaatgtttac
    26281 agacactatt aataagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26341 cccgctcggc ttctgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26401 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggcgc
    26461 gacctttggt atcgccttaa cgctggtcat tattgctggt tctgagctgt tcaccggaca
    26521 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26581 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtcttcgttg ccatgctcta
    26641 tagctggggt ggcggtagcc tgctgccggt agataccagc atcgttcact ccgtcgcgct
    26701 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26761 ggtttgcctg gctatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26821 ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26881 gctgttcgcc ctctcctggt ttggcaacca cagcgaagcc tacacgctgg caggtattgg
    26941 tcataacttg ctgtgggtga cgctgggtaa tactttatca ggtgccgtat tcatgggatt
    27001 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    27061 tgaaacagct gccggttaat tactaagggg tttttacgtg gatcatttgc ctatattttg
    27121 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    27181 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    27241 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27301 ccttctcgac acctgctggc tggcgattgc tgcgacggat gatgacgcgc ttaaccagcg
    27361 cgtcagtgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27421 cgccagcttt attatgccgt caattattga ccgctcaccg ctcatggtcg cggtctcctc
    27481 tggcggcacc tctccggttc tggcacgcct gttgcgtgaa aaacttgaat cactgctgcc
    27541 gttgcatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27601 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta acgaccgcct
    27661 ggcgcagtcg ctggcaaaca acgatcagaa agccattacc gaaacgaccg aacagttaat
    27721 caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt ggcgcaggtc cgggcgatgc
    27781 cgggctgctg acactaaaag gactgcaaca aattcagcag gcagatgtgg tggtctacga
    27841 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgcggatc gcgttttcgt
    27901 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag ataaaccaga tcctgctgcg
    27961 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggt ggcgatccgt ttatttttgg
    28021 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccgttct cggtggttcc
    28081 gggtattacc gcagcttctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    28141 ttatgcccag agcgtacgct taattaccgg acacttaaaa accggtggcg aactggactg
    28201 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    28261 gactattcag caaaagctga ttgaacacgg tatgcctggc gaaatgccgg tggcaattgt
    28321 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28381 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28441 cgataaattg aactggttct ccaaccatta atttaaccag ggccagagaa gtctggcctt
    28501 tttcgtttac aactctcttt atatattcag cagataaaaa cataccccct taataaatat
    28561 tatctgctaa tgggttatat tcaccgttac
//
LOCUS       DARNVM010000048        28068 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.048, whole genome shotgun sequence.
ACCESSION   DARNVM010000048 DARNVM010000000
VERSION     DARNVM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.048"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..305)
                     /locus_tag="SI621_001872"
     CDS             complement(<1..305)
                     /locus_tag="SI621_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HEI4064599.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     "
     gene            complement(483..1394)
                     /gene="gsiD"
                     /locus_tag="SI621_001873"
     CDS             complement(483..1394)
                     /gene="gsiD"
                     /locus_tag="SI621_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HEI4064600.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1397..2317)
                     /gene="gsiC"
                     /locus_tag="SI621_001874"
     CDS             complement(1397..2317)
                     /gene="gsiC"
                     /locus_tag="SI621_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HEI4064601.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2335..3873)
                     /gene="gsiB"
                     /locus_tag="SI621_001875"
     CDS             complement(2335..3873)
                     /gene="gsiB"
                     /locus_tag="SI621_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HEI4064602.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3893..5764)
                     /gene="gsiA"
                     /locus_tag="SI621_001876"
     CDS             complement(3893..5764)
                     /gene="gsiA"
                     /locus_tag="SI621_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HEI4064603.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLTGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5751..6716)
                     /gene="iaaA"
                     /locus_tag="SI621_001877"
     CDS             complement(5751..6716)
                     /gene="iaaA"
                     /locus_tag="SI621_001877"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEI4064604.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6920..8155
                     /gene="moeA"
                     /locus_tag="SI621_001878"
     CDS             6920..8155
                     /gene="moeA"
                     /locus_tag="SI621_001878"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEI4064605.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISADAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VEAGEWVEVEPFNALFGGL"
     gene            8155..8904
                     /gene="moeB"
                     /locus_tag="SI621_001879"
     CDS             8155..8904
                     /gene="moeB"
                     /locus_tag="SI621_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEI4064606.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCLFREMKLMRNPGCEVCGQ"
     gene            complement(8991..9653)
                     /gene="fsa"
                     /locus_tag="SI621_001880"
     CDS             complement(8991..9653)
                     /gene="fsa"
                     /locus_tag="SI621_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEI4064607.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEIVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9784..10683
                     /locus_tag="SI621_001881"
     CDS             9784..10683
                     /locus_tag="SI621_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEI4064608.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMKTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10689..13121
                     /locus_tag="SI621_001882"
     CDS             10689..13121
                     /locus_tag="SI621_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEI4064609.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13267..14082
                     /gene="ybiV"
                     /locus_tag="SI621_001883"
     CDS             13267..14082
                     /gene="ybiV"
                     /locus_tag="SI621_001883"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HEI4064610.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14234..15499
                     /locus_tag="SI621_001884"
     CDS             14234..15499
                     /locus_tag="SI621_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HEI4064611.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQRIQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15466..16626)
                     /locus_tag="SI621_001885"
     CDS             complement(15466..16626)
                     /locus_tag="SI621_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEI4064612.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16864..18165
                     /locus_tag="SI621_001886"
     CDS             16864..18165
                     /locus_tag="SI621_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064613.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPIKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18180..20543
                     /locus_tag="SI621_001887"
     CDS             18180..20543
                     /locus_tag="SI621_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEI4064614.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(20723..22318)
                     /locus_tag="SI621_001888"
     CDS             complement(20723..22318)
                     /locus_tag="SI621_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEI4064615.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            22537..23457
                     /gene="ldtB"
                     /locus_tag="SI621_001889"
     CDS             22537..23457
                     /gene="ldtB"
                     /locus_tag="SI621_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEI4064616.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23515..24633)
                     /locus_tag="SI621_001890"
     CDS             complement(23515..24633)
                     /locus_tag="SI621_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEI4064617.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLFVMLPAW"
     gene            complement(24630..25097)
                     /gene="mntR"
                     /locus_tag="SI621_001891"
     CDS             complement(24630..25097)
                     /gene="mntR"
                     /locus_tag="SI621_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEI4064618.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25283..25411
                     /gene="mntS"
                     /locus_tag="SI621_001892"
     CDS             25283..25411
                     /gene="mntS"
                     /locus_tag="SI621_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HEI4064619.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25683..27266
                     /gene="opgE"
                     /locus_tag="SI621_001893"
     CDS             25683..27266
                     /gene="opgE"
                     /locus_tag="SI621_001893"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEI4064620.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAMLLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGLIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPMQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27315..27830)
                     /gene="ompX"
                     /locus_tag="SI621_001894"
     CDS             complement(27315..27830)
                     /gene="ompX"
                     /locus_tag="SI621_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HEI4064621.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27883..27948)
                     /locus_tag="SI621_001895"
     CDS             complement(27883..27948)
                     /locus_tag="SI621_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064622.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
BASE COUNT         6838 a         7729 c         7016 g         6485 t
ORIGIN      
        1 agtaccagtc ccaccgagtc taaaaatggc atcatttcca ttttaaggat cacagcgcgt
       61 tgaatctcct ctgtattttt taataaatac agtgagtcct ttaatgtcag ggccgaacgt
      121 tcaatatctt taaagaaata gccaacgttt ttctgaataa gttcaaagcg gctatcggca
      181 cgttttaccg cgcgttccga taccagttga tagttaatga tgaaaaaaag gccaataaat
      241 gacagtgctg ccaataaaaa taaaattatc agccttatct ttatcttttc gtataaactc
      301 agcatgttaa tacatttaac aaaataactg tctgaacgct gtgccgctgc aaaaacagtc
      361 ccttgctgcg gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac
      421 acttgcttag ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt
      481 aactatcctt taattttcgg atccagcgca tcgcgtaaac catcacccaa caaattgaac
      541 gccagtacag tcagaaaaat agccagggcc ggaaaaatag cgacatgcgg cgcgataacc
      601 atatccgctc gggcctcatt gagcattgca ccccactctg gtgtcggcgg ctgcgcaccg
      661 aggccgagaa atgacaggct ggcggcagag ataatcgagg taccgatgcg catggtgaaa
      721 aacaccacga tagaagagac agtcccgggc aagatatggc gcaataagat ggtcatgtcg
      781 ctggcaccaa tactgcgcgc cgattcaata aaagtttgct gtttcaacac cagcgtgttg
      841 ccacgcacca gacgggcaaa agccggaatg gaaaaaatgg cgactgcaat aatcacgtta
      901 gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca
      961 aacagcacat cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc
     1021 agcaaaccca gcaacgtccc gatcgccgca ccgataaaca cggcaaacac gcccgccgcc
     1081 agcgagattt gcgcaccaac caggacacgg ctgaaaatgt cacgccccag tgaatcgacg
     1141 ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg
     1201 gcgtcatagg gagcgatcca acgtgcaaaa atggccacca caatcaataa aataacgaat
     1261 aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt
     1321 gtacgtacct ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag
     1381 ttaaatagtc gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat
     1441 ccaccactaa gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca
     1501 ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg tccaagtccg ggccagttga
     1561 aaactttctc caccacgata gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg
     1621 tcacaaccgg gatcatcgcg ttacgtagcc cgtgtttgag gacaacccag gtttcgctca
     1681 cccctttcgc cctcgcggta cgcatataat cttcgcttaa aacatcgaca aacgacgcgc
     1741 gggtaaagcg cgccatcacg gctgccactg ccgcgccgag cgtcagggag ggtaaaatgt
     1801 agtgctgcca actgtctgct cccacggtag gcagccagcc cagttcaacg gagaatacct
     1861 gaattaaaag catccccaga gcaaatgccg gaaacgagat ccccgacacc gcaatggtca
     1921 tactcaatcg atccggccaa cgattacgcc agacggcggc gataattccc gccgccatac
     1981 caaatatcac cgcccagacc atactggtta tggtcagcca cagcgttggc ataaagcggc
     2041 tggcaatctc atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca
     2101 cagcattgct gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct
     2161 gacgcaccag ttctataacc tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat
     2221 cgccgggcag catatgcaca aataaaaaca ccagcaccga gacgataaac agcgttggaa
     2281 tcaaccccag taagcgtttg ataacgtaat taagcattcc actccctgcg ttgcttattg
     2341 caaatccgcg tcttcaaagc tgaaaccggt gtctggcatg atccaaaaac cggtcaggtt
     2401 tttactgtgt gccgacacca gtttttctac caccagcggg atccacggcg actcttgcca
     2461 gatgatatcc tgcgccgcct tatataaacg ggtcttttct gccggatcat tagttttcag
     2521 tgcctgagcc aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt
     2581 cggtggccag ttctgcgagg caaacagcgg cgatagtgcc cagtccgctt cgccggttga
     2641 agccgaccag ccagtgtaga acatccgcac gccgctctct ttttgccctt taccttcaac
     2701 ttctgccgcc cgctgtccgg catccatcgc agtcacctgg gctttaatcc cgacctgcgc
     2761 taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca
     2821 cagcgtggta ttgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac
     2881 tggatcgtaa ggccacggtt tataactttg cgcgtaggcg atactcggcg gtaccacacc
     2941 agtagctggc gttgcatagc ccgcgaaggc cactttcacc agcgccgggc ggttaatggc
     3001 gtaattcagc gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact
     3061 gatataacgc tgcataattg acggactggc catcaactcg atatttttgt ttttctccag
     3121 cagtgcggcc tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat
     3181 tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa
     3241 tcctggctgc cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt
     3301 atccagttca tacggcccgg ttcccaccgg atgaaaacca atctccttgc catatttttc
     3361 cagcgctgcc ggcgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga
     3421 gaacggctgt ttgagggtaa tctttaccgt tgttggatcg atcgcttccg ttttggcaat
     3481 attcttatac aggttgtagc gtttaagatg attcgccgga tcgctggccc ggtccagatt
     3541 cgcttttacc gccgctgcgt tgaaatcggt gccatcctgg aatttaattc cttcccgcaa
     3601 tttcacggtg tatgtaaggc cgtcatcgga aacggtataa ctctccgcca gcacgttttt
     3661 cagtttcatc tctttatcca gaccaaacag cccctggtaa aacgatttcg ctacggcctg
     3721 agataacgtg tcatttgcgt catacggatc gagcgtggtg aagttcgatc ctaccgccac
     3781 caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc
     3841 cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac
     3901 gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca
     3961 acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca
     4021 gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt
     4081 tacgcgtata aggatgctgc gggttttcga agaccgcgcg ccgtggacca atttcaacaa
     4141 tttgtccgag atacatcacc gccacacgat gactaatccg ctctaccacg gccatatcgt
     4201 gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa
     4261 tctgcccgcg aatagaaaca tccagcgccg agacggcttc gtcggcaatg atcacttttg
     4321 gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat
     4381 gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc
     4441 gtgcagccgc ttctttacct gtcaataaac cgtgtacgcg cagcggttcg ataatcgaat
     4501 caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat
     4561 cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc gattcgctga ccgttaaaga
     4621 taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac
     4681 cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt
     4741 tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac
     4801 gggtgaccag attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga
     4861 tgggtggttc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat
     4921 ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat
     4981 gttgcggtgc atgaaaaatc tgttcgacgc tacccgtttc caccgcctcg ccctgataca
     5041 tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa
     5101 cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag
     5161 tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg
     5221 ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgga taacgtgaaa
     5281 gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg
     5341 cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag
     5401 taaataccgg gttcagcgat gtcatcggtt cctgaaatat catcgccata tccgcaccgc
     5461 gcacatggcg catttgtgca gcgctctgct cgctaagttc aatcacttcg cgactgcgcc
     5521 gccgcaacag catttgatcg cactgcacta aaccgcccgc ctgttccaac aagcgcatca
     5581 atgccagcgc agtcactgac ttaccagagc cggattcgcc aacaattgcc agcgtctcac
     5641 cgcgttgcag gctaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa
     5701 aggcaatatt cagattttca accgccagca catcaccggc atcaagttca tcactgtgtg
     5761 gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc
     5821 caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattgccttc atggtcgata
     5881 gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag
     5941 gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc
     6001 gcgcggatga agacttcgcc cgtgccggta catgaaaccg ccacactggc gttattggcg
     6061 tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt
     6121 ccgcccgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg
     6181 gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct
     6241 tcctcacgcg ctgccattag ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag
     6301 acgcgctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca
     6361 tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg
     6421 cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg
     6481 gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc
     6541 agacgcaccg cttccgtcac cacatccagc gcactttcgc ccgcttccag cattttctgc
     6601 ccggtttcaa caatggcaga cagcgcctcg atgtagcgta attcctgttg cagactcatc
     6661 tgcgcgcggc taattgcacc tgcgccgcca tgaattgcaa tgactgcttt gcccatagta
     6721 ttcatccatt aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac
     6781 tcattcaatt tttgaatata gaaagatgga tatggcatgt aaaggcagga gccagcgcta
     6841 cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat aataggcaaa ttcgattttg
     6901 cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct
     6961 taatgagatg ctttctcgcg tcaccccact gaccgcccag gaaacgctgc cactggtaca
     7021 gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg cttgatgtcc cggggtttga
     7081 taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct
     7141 gcccgttgcc ggtaaatcct ttgccggtca gccgtaccat ggtgaatggc ctgcgggtac
     7201 ctgcattcgt attatgaccg gtgcgccggt gccagaaggc tgcgaagcgg tggtcatgca
     7261 ggaacagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca
     7321 aaatattcgc cgtcgcggtg aagatatctc tgcggatgcg gttgttttcc cggcgggaac
     7381 acgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt
     7441 gattcgtaaa gtgcgtgtag cgctgttttc taccggtgat gaactccagt tgccaggcca
     7501 gccgctgggc gacggccaaa tctacgatac caaccgtctc gccgtacacc tgatgttaga
     7561 acagctggga tgcgaggtaa tcaacttagg gattatccgc gacgatcccc atgccctgcg
     7621 cgccgctttt attgaagccg atagtcaggc ggatgtggtg atcagttccg gcggtgtttc
     7681 agtgggtgaa gcggattaca ccaaaaccat tcttgaagag ctgggggaga tcgccttctg
     7741 gaagctggcg attaaaccag gtaaaccgtt cgcgttcgga aaactttcta atagctggtt
     7801 ctgcggcctg ccgggcaacc cggtttcagc aacgctgacc ttctatcaac tggtacagcc
     7861 tttgctggca aaactaagcg gcaataccgc cagcggcctg cccgcgcgcc agcgtgtgcg
     7921 cacggcatcg cctctgaaga aatcgccagg acgccttgat ttccagcgcg gcgtgctgca
     7981 acgcaacgcc gatggcgaac tggaagtgac gaccaccgga catcagggtt cacatatatt
     8041 tagctccttt agcctcggca actgctttat cgtactggag cgcgagcgcg gcaatgtaga
     8101 agcgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg
     8161 gaactcagcg atcaggagat gttgcgctac aaccggcaaa tcattctgcg cggttttgat
     8221 tttgacggtc aggaggcgct aaaagattct cgtgtgctga tcgtcggcct cggtggactc
     8281 ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac
     8341 ttcgacacgg tttcgctctc caatctgcaa cgccagacgc tgcacagtga tgccacggtc
     8401 gggcaaccga aagtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg
     8461 attacacccg tcaatgcact gctagatgac gcagaacttg cagcattgat tgctgaacac
     8521 gatctggtgc tcgactgtac ggataacgtt gcggtacgca atcaactgaa cgcaggctgt
     8581 tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg tcaaatcacg
     8641 gtcttcactt atcaggacgg cgagccttgc tatcgctgcc ttagccgttt gtttggcgag
     8701 aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt aattggttcg
     8761 ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa
     8821 atcgtcatgt acgatgcgat gacctgcctg tttcgtgaaa tgaaactgat gcgtaacccg
     8881 gggtgtgagg tgtgtgggca gtaattgcca cctcaggcca gataagacgt agcaggcatc
     8941 ggtgcactcc gccacgtcat cagcaattta tgaggatgca gaacgtccgg ttaaatcgac
     9001 gttctgccaa acgctcccag ccagtcctgt tcaaacttcg ccacagcggc atcaaccgcc
     9061 ggatagctaa tcatctgttg tgccacatcc agcggcagag taattgattc acaccctgcc
     9121 agtaagcagt ccagcgcctg acgcggggtt ttgaaactcg cggctaacac tttcgcctgc
     9181 ggcgcatgca ttttcaataa ctggtgtaag tcggtcacag tctgaatacc gctaccgccc
     9241 tgagcatcaa tacgattaac gtaaggcgca acatattccg cacctgccag cgccgacagt
     9301 agcccttgtg ccgcgccata taccgcggtt cccagcgtcg gaatcccttc cgcttttaac
     9361 atcttaatag ctgccagccc ctcagcggtc accggaactt tcaccacgat atccgcaata
     9421 atagaacgca atttacgtgc gtcattcacc atcccttcag cagtggtagc cattacctgg
     9481 gcaaacagac gcccctgacc gcccatcgct tcatgaagtt ccggaagcac aatctccagc
     9541 ggttttttac ccgcggcgat aatgcttggg ttagtggtca cacccgccag cggaaaaata
     9601 cgtgacagcg ccttcaccgc aacaacgtct gaagtatcca gatacagttc cataaccatc
     9661 ctcaaaatgt actttttgtc tgaatgtaga ctatcacggg aaaactctct gaacagttga
     9721 cgcacatcaa tctaactttc attcgaaagt attttaatgt ttgaacgaaa cagagaggca
     9781 actatgattt tcaatattca gcgctactcg acccatgatg gccccggtat ccgcacggtc
     9841 gtatttctta aaggctgttc gctgggctgc cgctggtgtc agaacccgga aagccgcgcc
     9901 cgcacgcagg atctgctgta tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct
     9961 aaggccgcgc cggaagtgat tgagcgcgcg ctgaatggct tgcttattca tcgggaaaag
    10021 ttaaccccgg agcatctgac agcgttaacc gactgctgcc cgacacaggc gttaaccgta
    10081 tgtggtgaag tgaaaagcgt tgaggagata atgaagaccg ttctgcgcga taaaccgttt
    10141 tacgatcgca gcggcggcgg cttaacgctt tctggcggtg aaccgtttat gcagccggaa
    10201 atggcgacgg cgctactgca agccagccac gaggccggca ttcatactgc ggtagaaacc
    10261 tgtctgcatg tgccgtggaa atatatcacc ccttctctgc cctatatcga tctgtttctt
    10321 gccgatttaa aacacgttgc cgacgcaccg tttaaacaat ggaccgacgg taacgccgcc
    10381 agggtgctgg ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg
    10441 ccgctgattc agggctttaa tgccgacgaa acctctgtaa aagccattac cgattttgct
    10501 gccgacgagc tgcacgttgg cgaaattcat ttcctgccct accacacgct gggcatcaac
    10561 aaatatcact tacttaatct gccctatgac gccccggaaa aaccgcttga tgcgccagaa
    10621 ctgctcgact ttgcccagca gtatgcctgc cagaaagggt taaccgcgac cttacgagga
    10681 taacaaccat gaccacactg aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa
    10741 atgcgctggt gcatattgtg aaaccgccgg tctgtaccga gcgcgcgcag cactatactg
    10801 agatgtatca acaacatctc gataagccga tcccggtacg tcgcgcgctg gcgctggcgc
    10861 atcacctggc gaatcgcact atctggatca agcacgatga gttgatcatt ggcaaccagg
    10921 caagcgaagt tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag
    10981 agattgatga tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac
    11041 gcgttctgca tgaagtgtgc ccgtggtggc gcggtcagac cgtacaggat cgctgctacg
    11101 gcatgtttac cgatgagcaa aaaggtctgc tggcgaccgg catcattaaa gcggaaggca
    11161 atatgacctc cggcgatgcg cacctggcgg tcaatttccc gctgctgctg gaaaaagggc
    11221 ttgatggtct gcgcgagaaa gtagcggaac gtcgctcgcg catcaacctg acggtgctgg
    11281 aagatttaca cggtgagcaa ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg
    11341 aacacattga acgtttcgct gccctggcgc gtgaaatggc cgcgaccgaa actcgcgaaa
    11401 gccgtcgcga tgagctgctg gcgatagcag aaaactgcga tcttatcgcc caccaaccgc
    11461 cgcagacttt ctggcaggcg ctgcaactgt gttacttcat ccagttgatt ttgcagattg
    11521 aatctaacgg tcactccgta tcttttggtc gtatggacca gtatctgtac ccgtattatc
    11581 gtcgcgacgt tgaactcaac cagacgctgg atcgtgaaca cgccatcgag atgctgcata
    11641 gctgctggct gaaattgctg gaagtgaaca agatccgctc cggctcacac tcaaaagcct
    11701 ctgcgggaag tccgctgtat cagaacgtca ctattggcgg gcaaaatctg gttgatggtc
    11761 aaccaatgga cgcggtgaat ccactctctt acgcgatcct cgaatcctgc ggtcgcctgc
    11821 gttccactca gcctaacctc agcgtgcgtt accatgcagg aatgagcaac gatttcctcg
    11881 acgcctgcgt acaggtgatc cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa
    11941 tcgtgatccc ggaatttatt aaactcggta ttgaaccgca ggacgcttac gactatgcag
    12001 cgattggttg tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt accggcatga
    12061 gctttatcaa cttcgcccgt gtgatgctgg cggcgctgga aggcggtcgt gatgccacca
    12121 gcggcaaagt gttcctgcca caagaaaaag cgttgtcggc aggtaacttc aacaacttcg
    12181 atgaagtgat ggacgcgtgg gatacgcaaa tccgttacta cacccgcaaa tcaatcgaaa
    12241 ttgagtatgt cgtcgacacc atgctggaag agaacgtaca cgatattctc tgctcggcgc
    12301 tggtggatga ctgtattgag cgagcgaaaa gtatcaaaca aggcggcgcg aaatatgact
    12361 gggtttctgg cctgcaggtt ggcattgcca acctcggcaa cagcctggcg gcagtgaaga
    12421 aactggtatt tgaacagggc gcgattggtc agcaacagct ggctgccgca ctggcggatg
    12481 acttcgatgg cctgactcat gagcaactgc gtcagcggct gattaacggt gcgccgaagt
    12541 acggcaacga cgatgatacc gtcgatacgc tgctggctcg cgcttatcag acctatatcg
    12601 acgaactgaa acagtaccat aacccacgct acggtcgtgg tccggttggc ggcaactatt
    12661 acgcgggtac gtcgtctatc tccgctaacg taccgtttgg cgcgcagact atggcaacac
    12721 cggacgggcg taaagcccac accccgctgg cagaaggcgc aagcccggct tccggtactg
    12781 atcatcttgg ccctactgcg gtcattggct cggtgggtaa actgcctacg gcagcgattc
    12841 tcggcggcgt gttgctcaac cagaaactga atccagcaac actggagaac gaatctgaca
    12901 agcagaaact gatgatcctg ctgcgcacct tcttcgaggt gcataaaggc tggcatattc
    12961 agtacaacat cgtttcccgc gagacgctgc tggaagcgaa aaaacatccg gatcagtacc
    13021 gcgatctggt agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc tctccagacg
    13081 ctcaggacga tatcatcgcc cgtactgaac atatgctgta atcttctcag cccggcggcg
    13141 tcgtcgccgg gctaaatcat tcacaccatc aattttagcc tcgctttcat tcgaaatata
    13201 atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg agccaggagc
    13261 acacctatga gcgtaaaagt tatcgtcaca gacatggacg gtacttttct taacgacgcc
    13321 aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa gcgcggcatt
    13381 gagttcgttg tcgccagcgg taatcagtat taccagctta tttcattctt tcctgagcta
    13441 aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa
    13501 ctgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga gttgctaaaa
    13561 gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc
    13621 cccgaagcat tcgtcgcact gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat
    13681 tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccgga tgaacaaatc
    13741 ccgttagtga tcgacaaact gcacgtagcg ctcgacggca ttatgaagcc ggttaccagt
    13801 ggttttggct ttatcgacct gattattcct ggcctgcata aagcgaatgg tatttcgcgg
    13861 ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac
    13921 gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac
    13981 attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg
    14041 attcaggctg tgctggataa cacatcccct tttaacagct gacctctcta ccggagccat
    14101 gcgctccggt tttcccttct ttttacatcc tgtacatcac atcaaatcat taaacttgca
    14161 ttaacttatt tataacttat attgtcacat gcaaatcatt atagtttcga atgaaagtta
    14221 atgcgaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc
    14281 agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt
    14341 taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca
    14401 gggcgatgcc gtctggccgg tgctgtctta tgccgatatc aaagcaggtc atgttactgc
    14461 agagcagcgc atacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg
    14521 cgaacaagcg ctaggctggg atcagtcgat gctggactat ctggaccgca accgctttga
    14581 cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga
    14641 gatttacccc atctactggt cgcaggcgca aatgcaggct cgccagagtg aagaaatggc
    14701 gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa agcaatggtt
    14761 taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc ccggaacgac
    14821 ctccaaaggt cttggagcgc ataccgactc cggggcactg gaacgctggc tgcttccagc
    14881 gtatcagcgc gttttcgcca acgtctttaa tggcaatctg gcgcaatatg atccctggca
    14941 tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt
    15001 gtttcggaca ttccagggct ggacagcgct ctctgatatg ctgcctggtc aggggctgct
    15061 gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga
    15121 tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtattgccgg tatcagagca
    15181 atggcatcca ctgttgattg aggcgttaac cagcattcca aaactcgaag ccggagactc
    15241 cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggctg
    15301 gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca
    15361 caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta
    15421 tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc
    15481 gttgggaatc gacagctgag tgtcgtacag cccttcccgt tccacggcgg tgttcccttt
    15541 tcctgggcga acccgccgca ctgactcccg caccgccaat cgtccgtgca gcagcagcga
    15601 gcccaccgga gcatccgggc gaatcagccg ctgttgcaac atttgcaccg cttcattgcc
    15661 tagctcatcg cgtggcacat gcacagccgt caggggaaca tcctcgattg ccgcaaggtt
    15721 aaagccgtcg atactcatca ccgagacatc ctgtggtacg cgcagaccac gacgatgtag
    15781 cgcatttacc gttcccgccg ccataaaatc cccgccgacc agaaacgccg tcggcaacgt
    15841 cttgccattg gcggcatcca gccacgctcc gaccttttct tccgcttctt tagcgctaaa
    15901 actgctgata ttcagcaaat gacggtcatg gtgaaaggcc agattatgct gacgccatga
    15961 ttctttaatc cctgcaaacc gctgctccat agtgtagcgg cgcaggcaca tcacattcat
    16021 cacctcccgg tgacccattt caaacagata gcgggcggcg aacgcgccaa ttaaccgatg
    16081 gtccggcgca atcgccggga ggcgcattcg ttcatcacgg cagttgatca gcacacaagg
    16141 cttagcgaga tcggcggcca gatcatggat gtgcggatcg tcaatcccca gcaggatcgc
    16201 cgcctgcgta tctgcttcat tcatccgcga gagaaatagc gcgggatcgc tgtcaaactc
    16261 ctccagcgcg cagtaacgca gccgcacttc atgcggcgat aacgctttgc tgatgctctg
    16321 gatcacacgg taataaaaga tatcagatcg ttggtcaaaa gcgcgttgcg gcgcgaaaat
    16381 aatcaggttg ttcagcaaca tacgacctgc tgccatgcca tccatcacgc ccaactcacg
    16441 cgcgcaggcc agcacctgtt cgcgagcgcg tgcactggta ttcgctttgc cagccagcac
    16501 ccgcgaaacc gtactcggcg atagcccggt acgtgcggcg atatcggcga ttttcagctt
    16561 cttgtccatt ttgtgatctg cctctaattc ggaaatgaca gaattttcat gagcaaaaaa
    16621 gcccattctg tctggcatta aatccatttc agtaatgtat atcagtcact catggaaaaa
    16681 cgtgcacaaa acccgctttc tttcgctttt agggtctctt actgtgaatt ggtagaacaa
    16741 cttccataca aaaataatgg aaaaacgaac aataaagact ttatttatca aaaagttaaa
    16801 agtggtgtga caactccatt ctgacgttgc cacacaaacc ctataccatt ctgtggagtg
    16861 catatgagtc agggtatcaa taacgatatg actgccagta aaccgcgcca tatcgtcaaa
    16921 aatctgcgtt ggtggatact ggtccttttt ttgctcggtg ttactgtcaa ctacatcacg
    16981 cgtaactcgc tgggtattct cgcccccgaa ctgaaaagca acctggggat taccaccgaa
    17041 caatattcct ggattgtagg tgctttccag ctcgcctata ccctattcca accgttgtgt
    17101 ggatggctga ttgacgtcat tggcctcaaa ctgggcttta tgatctgcgc gggactgtgg
    17161 gcgctggcct gtctgatgca tgcgggggcg ggaaactggg tacatctggc gctgttgcgc
    17221 ttctttatgg gcggtgcgga agccgcggca accccagcaa acgccaaaac catcggtgaa
    17281 tggtttccaa aatcagaacg cccgatcgcc gcaggctggg caggcgtcgg tttttccatc
    17341 ggtgccatgc tggccccacc gattatctat tttgcccacg cttcattcgg ctggcagggt
    17401 gcatttatgt tcactggcat cctggcgatg ctgtgggtgg tgctgtggtg ggcgttctat
    17461 cacaacccgg ataagcatcc gaacctcagc aaagccgagc tggactttat ccgccaggat
    17521 aacgaagcgc ccccgattaa attacccttt ctgaccgcac tgaaaaccgt gggcaaaaat
    17581 aaacgctttt acggtatcgc tatcccggcc tttatggcag aaccggcgtg ggcggtgatg
    17641 agtttctggg tgccgctata cctgtccaaa acctatggca tggacctgaa gcagatcgca
    17701 ttattcgcct ggttgccgtt cctcgccgct gatcttggca gtattgccag cggctatctg
    17761 accaaacttt acacccgcct gtttgggctg agtcgtgtga actccgttgt cgcatcatcg
    17821 gtcagtggcg cattcttgat ggtttcgctg gcggtgatgg cctttaccaa taacccgtat
    17881 atcgccatcg tccttatttc catcggcggc ttcggtcacc agatcatctc ctgcatgctg
    17941 agcgcactgg tggtggaatc cttcgacaaa ggtcagatgg ccactgttaa cggcatgcgt
    18001 ggttctgccg cgtggatcgc aagtttcctg ttctccctga ttatcggggt taccgctgac
    18061 aaaatcggct ttaaccccct gtttatcgcc atgggcttct ttgacctgat tggcgctttc
    18121 ttcctggtca cctttattgc tgaacgtcgc gcaaaacgcg cctgagagtg gatgtaaaca
    18181 tgaaaacgct gaaaaactgg accctgcaca aacagtctgc ccaccacgtt gaactgctgg
    18241 tcgatggcca gcatatcctg tgcctgtacg tgctggaaga aaatctgttc cgcgtgctgt
    18301 taaaacgcaa aggcgagctg gcgctggatc gcacctggag cattgctccg gcgcaggatg
    18361 tgccatggga agggcgcagc cgcgaagatc tcagcggctt tagcctgccc gcgtggacat
    18421 tgatccagca cgacgacacc gtggttatcg ccaccgagtc actgcgtgcc accgttcacc
    18481 agccgctgtg gctggagtgg cactatcgta acaacgaagg cgaatggctg ccgttggtga
    18541 atgaccgccc gaccagcgct tatctgctga atgcccacgg cgatggcgtg gcacactatc
    18601 agagccgtcg taaagatgaa cgcttttatg gcctcggaga taaagccggc gacctgcaac
    18661 gcaacggtaa acgttacgag atgcgtaacc tcgacgcgat gggctacaac gccgttagta
    18721 ctgacccgct gtacaaacac attccattca ccatcacccg ccgcgatgac attagcttcg
    18781 gcctgtttta tgacaacctc agcagctgct ggctggatct gggcaacgag atcgacaact
    18841 accacaccgc ctatcgccgc tggcaggcgg aagcgggtga tatcgactac tacctgttta
    18901 ccggcgaacg cgtacttgat atcaccaaag ccttcgtacg cctgaccggc aaaaccctgt
    18961 tcggaccgaa atggagcctg ggctacagcg gttcaactat gcactacacc gatgcgccgg
    19021 acgcacaaaa tcagttgatg aactttatcc gcctgtgtga agaacatgcg attccctgcg
    19081 attcgttcca gctctcttcc ggttatacct caattaacgg caagcgctat gtgttcaact
    19141 ggaattacga caaagtgccg cagccgaaag tgatgagcca ggcatttcac aatgctggtt
    19201 tgcacctggc cgccaatatc aagccgtgcc tgttgcagga tcacccacgc tataacgaag
    19261 tggcagaaag aggactgttc atccgtgatt cagaaacaga tgaaccagaa cgttccagct
    19321 tctgggatga cgaaggttct aacctcgact ttactaatcc gcagaccatc cagtggtggc
    19381 aggaaggcgt gaccacacag cttctggaga tgggtatcga ctccacctgg aacgacaaca
    19441 acgaattcga agtatgggac ggtgaagcac gctgtcacgg ctttggtcag acgatcgcga
    19501 tcaaacatat tcgcccggtg atgccactgc tgatgatgcg agcctcgatg gaagcccagc
    19561 aacgtttcgc tccggaaaaa cgtccgtact tgatctcccg ctccggctgc gcgggaatgc
    19621 agcgctatgt gcagacctgg agcggcgata accgcactag ctgggacact ctgcgctaca
    19681 acacccgcat gggtctgggg atgagcctct ccgggctgta taacctcggt cacgacgtcg
    19741 gcggtttctc cggtgataag ccggacccgg aactgtttgt acgctgggta caaaacggcg
    19801 tgatgcatcc gcgctttacg attcactcgt ggaacgatga ccataccgta aacgaaccgt
    19861 ggatgtatcc gggcgtcact ccggcgatcc gcagcgctat tgagttgcgc tatcgcctgc
    19921 tgccctactt ttataccctg atgtggcagg catacgccga tgatgaacca atgctgcgtc
    19981 cgactttcct tgaccacgag cacgacgttc agacttttga agagtgtgat gacttcctgc
    20041 ttggtcgcga tattttggtt gccagcgtcg tagagcaagg cgagcgccag cgccgtgtat
    20101 ggctgccaga taacgaaact ggctggtacg atttctacaa cggcgaatgg ttcagtggcg
    20161 gtcagtggat caccctcgac gcaccgctgg agaaactgcc gctgatggtg cgtgccgggg
    20221 ctggtctacc gctcagcaaa cgcatcacct atgtgagcgc agaacaagac gatacccgcg
    20281 aactgaaact gttcccgctg aaaggtgtgg gcacaacgtc cggcctgctg tttgaagacg
    20341 atggcgaaag ctggggctac caaactggta atgcgctttg ggtggaatgg gaaatggttt
    20401 gcgatggggc gaccatcaac ctgaaagtca acgcgcgcgg ggattatcga cctgcatgga
    20461 gtgcactgaa agtgtcatta ccagtggaag aaaaacgcac gctgctggtc aatggtgttg
    20521 aagggagtga atggatgcgt taaatcatta ccggggccgg gaggcgactg cgccttaccc
    20581 ggtccagcca tcgaatcctt tacctcagta tttcacgctg caggaaggca gcaaacctgt
    20641 aaaccccggg agcttaaata agtaagtgac cggggtgaac aggtgaagcc aatgcatctg
    20701 ccgcatgaaa gacggcggga aattaaccgt cgatcccttt actacgcagg taatcttcgt
    20761 aattaccgct aaagtcgatc acgcgttccg gggtgatttc cagaattcgg gtcgccaggg
    20821 agcttacaaa ctcacggtcg tgagacacaa agatcagcgt gccctgatac agttccagcg
    20881 ccatgttcag cgactcaata gattccatat ccaggtggtt ggtcggttcg tccatgatca
    20941 gaatgttcgg cttctgcatc attaacttac caaacagcat ccgccctttt tctccaccgg
    21001 aaagcacttt cgctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc
    21061 tgcgtaccgc ctgctcatca tcgccttcct gcttccactg gctcatccat tcgaacacgg
    21121 tcagatcatt ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgtgcgtttt
    21181 cagaccattt tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg
    21241 ttgatttacc gacgccgttg gtacccagta cagccagttt ttcacccact tccagcagca
    21301 ggttgagatt tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca
    21361 gcgcgttacg gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg
    21421 aggctttcac ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc
    21481 gagatttcga ggcgttggcg ctaaagcggc taacgaaaga ttgcagctca gcaatctgcg
    21541 ctttcttctt ggcgttatca gccagcagac gctcacgcgc ctgggtcgcc gccgtcatgt
    21601 actcatcgta gttacctgga taaacgcgca gctcgccgta atccaaatcc gccatgtggg
    21661 tacaaaccat gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct
    21721 cgttcagcac ctgttccagc caacgaatgg tgtcgatgtc gaggttgttg gtcggttcgt
    21781 cgagcaggag aatgtctgga tcagcaaaca gcgcctgcgc cagcagcaca cgcagcttcc
    21841 agccaggagc aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa
    21901 gcaacagttc accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt
    21961 ccagatcggc cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt
    22021 cacgctcctg tttcacttcc cacaactctt tatgccccat gatcaccgta tccaacacag
    22081 tgaactcttc aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga
    22141 gggaaacgtt acccaacgtc ggctctaagt cgccgccgag gatcttcata aaggtggatt
    22201 taccactacc gttcgcgcca atcaggccgt aacggttgcc gccgccaaat ttgacggaaa
    22261 tgttttcaaa caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag
    22321 gcgtatcctg aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc
    22381 ctgctcctcg cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat
    22441 ctgatttcac tgtgagcagg aatgaactta taatgcgctt ccaatactct aatattctca
    22501 acccaatggc ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta
    22561 ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct
    22621 ctgccaaccg acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt
    22681 aacactcagc cgctggagta ttttgccgcc gagtaccaga tggggctttc caatatgatg
    22741 gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg
    22801 cagcagctga tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg
    22861 cgtctgtatt actatccgaa aggaaccaac accgttatcg tgctgccgat cggcattggt
    22921 cagttaggca aagacacgcc tatcaactgg accaccaaag ttgagcgtaa gaaagcaggc
    22981 ccgacctgga cgccaaccgc taaaatgcac gcagagtacc gcgctgcggg cgaaccgctt
    23041 ccggctgtcg ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt
    23101 cgcctgtacg ctatccatgg caccaacgcc aacttcggta tcggcctgcg cgtaagtcat
    23161 ggttgtgtgc gcctgcgtaa cgaagacatc aaattcctgt tcgagaaagt gccggtcggt
    23221 actcgcgtac agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt
    23281 tatattgaag tccataatcc gctgtctacc accgaagccc agtttgaagg tcaggaaatt
    23341 gtgccaatta ccctgacgaa gagtgtgcag acagtgaccg gtcagcctga tgttgaccag
    23401 gttgttctgg atgaagcgat caaaaatcgt tccgggatgc cggttcgtct gaattaatcg
    23461 tcaaaacgta aaacagaggc ggactcatca tccgcctttt ttatttgcca gatcctacca
    23521 ggccgggagc ataacgaata aaacgtatcc caccagcgcc gcccacaaca gcatcggtat
    23581 tgaatagaga tggaaacgcc accagatacg gcgatcgttc gccatccgta acgcaatcaa
    23641 atttgccagc gatccgggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa
    23701 taaagacggc ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg
    23761 cgataaaccg attgccgtta accataaccc gggttcagat aaatgactca cgttacccaa
    23821 cacaccttgc aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa
    23881 caccagcagc agcgtccagt ccacactgag caccacgcga cgtgccagca gcgcaaagcc
    23941 cgccgccaca atcaccagtc cccacagctc ttgttttaac tccagcgccg tcagaaagac
    24001 gatatacagc cccagacaac tccacaccag ccgcggtttc cactccggag tttgcacccc
    24061 cgtatggtat tgcagtgcct ttccagggaa acaacaccag cacaggagca ggagcgtcag
    24121 catcattgcg ccagccagtg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga
    24181 acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt
    24241 gactgccagc gcttcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag
    24301 cgtgatagtc agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag
    24361 cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac
    24421 cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat
    24481 caggccgctt aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc
    24541 aaaaggcacg aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc
    24601 gccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa
    24661 cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg
    24721 gcgatttccg gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg
    24781 cggctttcct gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc
    24841 atttcaatca gccccatggt tgccagtcgc ttaagcattt tagccaccgt cggttgcgaa
    24901 actcccaaac gagcagccat gtctacctga cgagcttccc ccacttccct gatcaagtca
    24961 gaaatcagct caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg
    25021 aacccttcaa cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta
    25081 cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt
    25141 cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc
    25201 tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg
    25261 gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca
    25321 ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa
    25381 accgcagcaa gataaacctt ccgataaata gcggcgtcac ggtacgccgc ttcactcctg
    25441 cttttatgca ggcacaacgc gttttggtct gaaaaacccc actttttgtc ggatttgcaa
    25501 tccccttcgc aaaagatttg ttcgtcagta gttgacctga acggcggcgc gctctatctt
    25561 cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct ctccacgcac
    25621 tcttgcaggt attgaccctt gacgccaggg taagcacatg gcgtttgtta cgatagtggc
    25681 atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga
    25741 ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttat cccttcagtt
    25801 tgctctacac cgctgcgttt acggctatgt tgcttttgct atggcgaaca ttgcctcgcg
    25861 tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc
    25921 aggcctacgg cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag
    25981 agtcgaccga aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt
    26041 ttgcgctcgg cttaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata
    26101 ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccatgcaaa
    26161 acctggcctg gggcggcgta tttaaactga aagataccgg ctatccggta tttcgttttg
    26221 ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac
    26281 tttccggaat gaaagatacc tggacggtca ctgccgttaa gccgaaatat cagacctatg
    26341 tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg
    26401 acaatacccc gtttgccagc agtgttaacg gtttgatatt tgctgactac attgccgcca
    26461 gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac
    26521 agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt
    26581 ccaaccaggg ccaaatcggc gaatacgata ccgccatcgc cagcatcgcc aaacgtgcag
    26641 atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag
    26701 cgttactgga tatgaccgct caagtgctgg cacaagagca ctcgcaaccg cagctgattg
    26761 ttttacatct gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa
    26821 cctttgtgca atcgaaagaa acgtcgtgct atctctatac catgacgcaa acagacgatt
    26881 tactgcgtaa gctgtacgat cagttacgca acagcggcag cagcttctcg ctggtttact
    26941 tttctgacca cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg
    27001 atgataaata tcagcaaaat ttccaggtgc cttttatggt catttcgagc gacgataaag
    27061 cgcatcgggt gattaaagcc cgccgctctg ccaatgactt cttaggcttt ttctcccagt
    27121 ggacgggaat taaagcgaag gaaattaaca tcaaataccc gtttatatct gagaagaaag
    27181 ccgggccgat atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa
    27241 ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttta
    27301 tatcaccgaa gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg
    27361 ctacgaatac ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac
    27421 tgcagaccag caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat
    27481 tcagtggtct ggaatttacc ataacccaca cccactacac cgtagatgct tgcccagtcg
    27541 ttaatgcggt aagccggacc agcagtgatg ccgtagtact ggtttttgtt gtagtcacca
    27601 gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg
    27661 ttgtcttctt catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga
    27721 gcgtcgctct gtgcgtaacc gccagttacg gtagaagttg cagctacgga agtacctgcg
    27781 gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca
    27841 aatgtgattc aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg
    27901 cgatttgtga tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc
    27961 atctaaattt aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc
    28021 attcatccag attattccta atcaccagac taatgattcc atcaatcc
//
LOCUS       DARNVM010000049        27758 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.049, whole genome shotgun sequence.
ACCESSION   DARNVM010000049 DARNVM010000000
VERSION     DARNVM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.049"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            178..753
                     /locus_tag="SI621_001896"
     CDS             178..753
                     /locus_tag="SI621_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013316556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEI4064623.1"
                     /translation="MATKNLVFAKYQYQVEFAEMVISYIKSTQLIINYVSDKNKTINE
                     KTILECHKALFGHIYDWAGEYRNHPVVVGDKERPTMEHNEVKKSLKACLRGCTKKELS
                     KINSKAELVHKLATLHAEIAWIHPFQDGNGRSIRLFLQIVATTMGYEFDMEKLDGDVR
                     NKRAYHYAVRRAIHDSNRNLIALISRAIKEL"
     gene            complement(750..947)
                     /locus_tag="SI621_001897"
     CDS             complement(750..947)
                     /locus_tag="SI621_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064624.1"
                     /translation="MSKKVITIQVRGGHAGAKPVRRSKLEQSVNRSLRASFSLEGNHI
                     TNTSWSKMSQAARFLTRVAVA"
     gene            complement(1124..3406)
                     /locus_tag="SI621_001898"
     CDS             complement(1124..3406)
                     /locus_tag="SI621_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HEI4064625.1"
                     /translation="MAVKASGRFVPPSAFVAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNSRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWSDPEVVRALRGALKHGRRTPSRQ
                     QRNGSPLKPHEIAPSARLTRTERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVVDEWRWYIVDGRR"
     gene            complement(3396..3671)
                     /locus_tag="SI621_001899"
     CDS             complement(3396..3671)
                     /locus_tag="SI621_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HEI4064626.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YQKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3668..3892)
                     /locus_tag="SI621_001900"
     CDS             complement(3668..3892)
                     /locus_tag="SI621_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEI4064627.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3895..4194)
                     /locus_tag="SI621_001901"
     CDS             complement(3895..4194)
                     /locus_tag="SI621_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HEI4064628.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(4194..4418)
                     /locus_tag="SI621_001902"
     CDS             complement(4194..4418)
                     /locus_tag="SI621_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HEI4064629.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIHHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4482..4982)
                     /locus_tag="SI621_001903"
     CDS             complement(4482..4982)
                     /locus_tag="SI621_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HEI4064630.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(5152..5424)
                     /locus_tag="SI621_001904"
     CDS             complement(5152..5424)
                     /locus_tag="SI621_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064631.1"
                     /translation="MEVNDYVIQYPIDAVHTVKFAELLGKPETAVVKMVKENKLPVIE
                     LRDPSKPNARVGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5577..5870
                     /locus_tag="SI621_001905"
     CDS             5577..5870
                     /locus_tag="SI621_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4064632.1"
                     /translation="MQTFEKLKAIRKAEGLTQAKFSEISGIALGTVKNYESGHKDPGL
                     SIVMRVTNTPLFKKYTLWLMTGDTSPQAGQIAPALAHIGQKPTESDHSGKQTG"
     gene            5940..6923
                     /locus_tag="SI621_001906"
     CDS             5940..6923
                     /locus_tag="SI621_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEI4064633.1"
                     /translation="MSIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEAVAFERYTIA
                     NASQKEWAGQRADRRTLTELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMN
                     KRALMDYRSMRLRDGISAATINRDIYRLSGMFTKLIQLDEFSGQHPIHGLPPLTEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGLTIR"
     gene            complement(6924..7160)
                     /locus_tag="SI621_001907"
     CDS             complement(6924..7160)
                     /locus_tag="SI621_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064634.1"
                     /translation="MELFVISSKIPLCKRIKLVISLFKGVMTILCGYNWSLKFIPAEK
                     AQVDGLSFDVYQDDVTSQDVLIVLVDNKRTMLPE"
     gene            7144..7479
                     /locus_tag="SI621_001908"
     CDS             7144..7479
                     /locus_tag="SI621_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064635.1"
                     /translation="MTNSSIRKIHCRASGRNIAGLAKELDTAAIAYQRGPICFGAGTT
                     EYLNIVLPFATAAFGAVAGVFGAYLQRGKKLRVVFDDGKVKELDATNYTPDELVSAVQ
                     KIKEIDLLD"
     gene            complement(7671..8171)
                     /gene="cpxP"
                     /locus_tag="SI621_001909"
     CDS             complement(7671..8171)
                     /gene="cpxP"
                     /locus_tag="SI621_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HEI4064636.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8321..9019
                     /gene="cpxR"
                     /locus_tag="SI621_001910"
     CDS             8321..9019
                     /gene="cpxR"
                     /locus_tag="SI621_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HEI4064637.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            9016..10389
                     /gene="cpxA"
                     /locus_tag="SI621_001911"
     CDS             9016..10389
                     /gene="cpxA"
                     /locus_tag="SI621_001911"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HEI4064638.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSNTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10494..11168)
                     /gene="yiiM"
                     /locus_tag="SI621_001912"
     CDS             complement(10494..11168)
                     /gene="yiiM"
                     /locus_tag="SI621_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HEI4064639.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDEQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11317..12300)
                     /gene="kdgT"
                     /locus_tag="SI621_001913"
     CDS             complement(11317..12300)
                     /gene="kdgT"
                     /locus_tag="SI621_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HEI4064640.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARVAKIEILGTVK"
     gene            complement(12560..13180)
                     /gene="sodA"
                     /locus_tag="SI621_001914"
     CDS             complement(12560..13180)
                     /gene="sodA"
                     /locus_tag="SI621_001914"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HEI4064641.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13465..14499
                     /gene="rhaT"
                     /locus_tag="SI621_001915"
     CDS             13465..14499
                     /gene="rhaT"
                     /locus_tag="SI621_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEI4064642.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIFHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(14496..15362)
                     /gene="rhaR"
                     /locus_tag="SI621_001916"
     CDS             complement(14496..15362)
                     /gene="rhaR"
                     /locus_tag="SI621_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HEI4064643.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQRAIPGFSASAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDEFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(15418..16254)
                     /gene="rhaS"
                     /locus_tag="SI621_001917"
     CDS             complement(15418..16254)
                     /gene="rhaS"
                     /locus_tag="SI621_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HEI4064644.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQKTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16542..18011
                     /gene="rhaB"
                     /locus_tag="SI621_001918"
     CDS             16542..18011
                     /gene="rhaB"
                     /locus_tag="SI621_001918"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEI4064645.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLM
                     ADYFGYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLSGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPAEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            18008..19267
                     /gene="rhaA"
                     /locus_tag="SI621_001919"
     CDS             18008..19267
                     /gene="rhaA"
                     /locus_tag="SI621_001919"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEI4064646.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNGSELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYSARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            19350..20174
                     /gene="rhaD"
                     /locus_tag="SI621_001920"
     CDS             19350..20174
                     /gene="rhaD"
                     /locus_tag="SI621_001920"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEI4064647.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTHEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            20184..20498
                     /gene="rhaM"
                     /locus_tag="SI621_001921"
     CDS             20184..20498
                     /gene="rhaM"
                     /locus_tag="SI621_001921"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEI4064648.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(20539..21933)
                     /locus_tag="SI621_001922"
     CDS             complement(20539..21933)
                     /locus_tag="SI621_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEI4064649.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            22410..22856
                     /locus_tag="SI621_001923"
     CDS             22410..22856
                     /locus_tag="SI621_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEI4064650.1"
                     /translation="MAALTASCIDLNIQGNGTYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            22867..24318
                     /locus_tag="SI621_001924"
     CDS             22867..24318
                     /locus_tag="SI621_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HEI4064651.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGIENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDHIFSE
                     LPTNSQFFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELAHEH"
     gene            24308..25378
                     /locus_tag="SI621_001925"
     CDS             24308..25378
                     /locus_tag="SI621_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEI4064652.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSILNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVVALKSCATH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKINAFSQFRQVD"
     gene            25378..27126
                     /locus_tag="SI621_001926"
     CDS             25378..27126
                     /locus_tag="SI621_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HEI4064653.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGNAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERGFCLASRPGLGHFIDETEEKRIILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGSEIKQIV
                     EYSGLFLGDNAVRTLTGLIEKQHQQAQVICVDNVLALLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSDITAISQE
                     ITPLLIVNNSHYLLDDAVKNSITIKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHFSNVRGENWQHITQQICAQLVTQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVVNHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLKGYEAFMELLHKG"
     gene            complement(27182..>27758)
                     /locus_tag="SI621_001927"
     CDS             complement(27182..>27758)
                     /locus_tag="SI621_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HEI4064654.1"
                     /translation="ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVA
                     ESYHAAIQEINDKRQLAELKNIEQREGKTFHYYSLAVMISAKQINNLISQEKFDVDAA
                     MKKVSELETLVAQAKEADKGGMNFSFINSADQYQLEAKKYVRRVRDKVPYSDWDKEQL
                     QDANTSWMVDDSFPRALREYNEMVDDYNSLR"
BASE COUNT         6986 a         7027 c         6804 g         6941 t
ORIGIN      
        1 gccctttttc tttttggttc aattgttggt gtagacaagc taaaaaaaga aacttatttt
       61 cacatattgc ctcacgacaa aaactttcca tttcaacgca atcatgtaat caaagtaaaa
      121 caccaaaaag acaatatttt tcttttacgt gacgggaaaa atgaaagata tgaatacatg
      181 gcaacaaaaa atctagtatt tgcaaaatac caataccaag ttgaatttgc cgaaatggtt
      241 atttcatata tcaaaagtac gcagctcata ataaattatg tctccgacaa aaataaaaca
      301 attaatgaaa agacgatctt agaatgccat aaagctcttt ttggccatat ttatgattgg
      361 gcaggtgaat acagaaacca tcccgttgtt gttggagata aagagcgacc aacaatggaa
      421 cataatgaag tcaaaaaatc attaaaagcc tgtcttagag gttgcacaaa aaaagaactt
      481 agcaaaataa attctaaagc agagttagtt cataaactag ctactctcca tgcagaaatc
      541 gcatggatcc acccctttca agatggtaat ggtcgttcaa tacgattatt tcttcagatt
      601 gttgcaacta ctatgggtta tgaatttgat atggaaaagc tagatggaga tgtgcgaaat
      661 aaacgtgcgt accattatgc tgtaagacgc gcaatacacg acagtaacag aaatctgatt
      721 gcccttattt caagggcaat caaagagctt taagcaacag ccactctggt cagaaaacgt
      781 gcagcctgcg acattttaga ccatgaagtg ttggtaatgt gattaccttc cagagaaaac
      841 gaagcacgca gggaacgatt aacactttgt tcaagttttg aacgacgaac aggcttagca
      901 cctgcgtgcc cacctcttac ttgtatcgtg ataacctttt tgctcatatt agtctctcta
      961 gtggttaaga acatcataaa tcaacagcta atgataacca ttatcatctg gaatcatcat
     1021 agccggctgg cgattctacg cctgattgtt catatctaca acaactactt tatacataca
     1081 aatctactta cctaccaata acagtgttca catcttcaac ttttcaccgc ctaccatcaa
     1141 ctatatacca ccgccactca tcaacaaccg gatacgtgaa ttttttccca tcataattta
     1201 cggtcgcgcc acgcgccagc gcctcaagct cccatcgctg cggcctgata ccgttctgag
     1261 caaggtcaac gcggatacgg gtaatttgca ttctttccgt ccgggtcagt ctggccgatg
     1321 gtgcaatttc atgtggtttt aacgggcttc cgtttctttg ctgacgactt ggcgttctcc
     1381 gaccgtgttt taatgcacct ctgagcgccc tcacgacctc cgggtcactc cattcgataa
     1441 caccgtcatc aaccagatta agcactgctg cggcgtgctc agaaggtgtg ggagccggta
     1501 acgaagtatc accaccggtg agttttccac agttattgac aggactccga ggcgcggcga
     1561 tgccgctttt taacgtcaaa ggctcaacga ccggaacttt cggcacaatg cgccagtccg
     1621 tcgttctggt gatatgaata tgacgcgcgc cgagatgcgg cgcgtaaatg ccgaccactc
     1681 tctcgacttc ttcctcatac tcgttaacgt catccgacgg gctacgggca accctgacag
     1741 tctgacaatc gcgcgggaca tttgccccac cctgcgcgct gatatacaac gcaaaatcac
     1801 cactgtctgc ggcagcgcga gcagcctcga cgcgttcgtc aaactcatca gcaatactga
     1861 cgccgcgagg caatttgcgt agttcacggt aagcccccat tgtcggcagt ccaaccgttt
     1921 taaattgcgg gatgcgccac gttgacgccc atgcagtaac agccgcggca gtatctttca
     1981 gaggtctgcc ggtatcgtta tcgagctgac catccagtgc atagccgtcg atgttttttg
     2041 agatgtattt cgcgatatac cccgcagcac cgccccgatt aaggtgtttt gcctgaaaac
     2101 ggtttcgcgc tgctcctctt tcgtcgccat cctctttgag cgcatagcga cgcatgattt
     2161 cgataatctg gttacgctgg cgcgaattac aaaaaagcat catatgccag tgcggcgttc
     2221 cgtcgtggtg tggctcgacg acacgcaaac cgtagacctg taaatcatta tccttgaatg
     2281 ccgtgcgcat caggctccag atgcggcaga gataacgctg cgcatccttt ggattaaatg
     2341 cctcatcgtt ccagccgtga ttaagctgaa cggttttact ttcgcctttt ctgacctgac
     2401 gagtcgggtg atactttgac ggcgcagtca gcgtgataaa catccccaca tcaccctctg
     2461 cggcggcgta acgctcaata ccggcaatgg tgttcatcag ctccatccgg cgaatttcag
     2521 gattagaaat actgcccatc accttactga taaggtcgat gcgctcgccg gtttccctgt
     2581 tttcaaggtc acacgattta agaaattcca gatttgcctg gcggcgcgca cgcacatcac
     2641 gaatggcgtg tttactggca taaggagaac ggtctttatt gacctccccg acagctatca
     2701 gtaacgcttc atgccagcgc atacgctggc ctttaagctg atgagtccac cactcatcgt
     2761 taaacagacg ggcaatggca gaatatgcct gcctcgtggt catctgccct ttacggtatt
     2821 ttttccagta gagaggggaa atattgaaag cacgtgcagc gccagcaaca tgaccataga
     2881 ggtgagcctg cgcctcatcc gtaaacagcg attctttttc gccatgcgca tccacccagg
     2941 catcgctgag ttcctcatac atcatgaaaa gctgcgatga gatacgggca gcaaactttt
     3001 tcagctcctt gtcattcatt cccggtaggc gcgcatagtg gtcacgctct gccagaaaca
     3061 gcaacgacgc gtcggtgttc atttcatggc gctgattcac acgctcaatg cgcggccata
     3121 aacgacgctg aaaagtggat gtgaggaaat aaaacccgtg caccgggctt ttattgcgcc
     3181 ggatgtagtc atagcgtgaa gtaaacagcg agcgcaaaaa gtaaggcagg cggttaatcg
     3241 tggataaaac accttgcacc tgacgcatct cgtcacgtgt aaggggtctt tcgcgcccga
     3301 cagcctcgcg tggcgcgttc catgcataag caccggtaaa cgccttaccg gtgcctgcaa
     3361 caaatgctga cggagggaca aaacgcccgg aggctttaac ggccatatga gccaaaagcc
     3421 tctgaacaac gcttgctgag ttgctcaacc tgcgcgttta aatcagcaaa agactttgcg
     3481 cttccggtca gaatatcgtg atgcatcagg ccggaaacga gctggcttaa tttctggtaa
     3541 taaccaacca ccgccagcca ttcctgaccg gcgtttttac cgctttccgc tctctttttc
     3601 tcgtggagaa taaactggaa gctgtcactg gtaacgacat aacgttcgcc aatttcaata
     3661 cgaatactca tgccgttctc cggtaatgtt tgttttttgc ttcaaagact gactgacagg
     3721 aaacacaacg cgtggctgac ggataagccg cacgacgggc agcaggtatt ggcgcgtcac
     3781 actcttcgca aaccagcgca gaagcaccgc aatgttttac ccttgccgcg ttaatctggc
     3841 gctccagtaa ttcagcctgt tgttcctgaa taaaatctac gttgtccggc attatcagct
     3901 ccttttatcg ttaagtttcc tggatacatc agcgcaataa ctggcaagtt ctgtcgttaa
     3961 ttttgtcagt tcatccacgg aggaaatttg cttgtggaat acagcgcgtt taacaagtaa
     4021 attgaccaca tcagacagga ggtttaattc attctgataa atcgcgataa cagattcagt
     4081 gatgtcgcgt ttttctttat caagacaaag ttgaataaga gacaaatcac cattttccat
     4141 aacggcgatt tttaaggcgt tattcagtaa tacaactgaa tgagaacagg acatcaaagt
     4201 acctccccgc gagacaatcc gatattgtga aatttttccg actcctgact gagcagctcg
     4261 actatctcca cgcgggataa ctccgccttt gtgatatggt gaatcatggc gtcaagatga
     4321 gaagaaaagc gtgtcgcagc gtcggcctgt gcttcggttc tggcctgttg cagcagtaat
     4381 gcgtatttac cgcactgatt ttcagaaact gtatgcatga ctttctccaa gcaaaaagaa
     4441 gccccgcacg attaagtgcg ttaaaaactc tggttaatta cttaatgcag atattgctct
     4501 ggttttaccg acgtcagaat tgtcggtgca tactcaaaca gactgaataa ttcacgtaat
     4561 gcacggaata aagcatcacg ccagtaacat gactcttcat taattcgcca gtatggctgg
     4621 ttgaattctt tttcagtcaa tccggcatgc ataaataaag tacgacgctg actgactgtt
     4681 aaaaaactaa tatatgcata ctcacttgcg ccaacctgac ggcgttttga gaatgcccca
     4741 cgcaattcat caattgcaca aaccagccgt tcacgttcga cgtcgttcat ttcttcaaaa
     4801 cgcatcgttg cgtgacgctg ttttaactgc gcatgaaagc aaaccgttaa ccgttcgcgc
     4861 tccatcatct gattataata atcacatgta tcctgccagc gagggacggc cagatgctta
     4921 ccaattatcc ggcgcatagt tgctggctgt ttttcgacga gattgagcgt catcactgtc
     4981 atttccagac cctccggctt ttcagaaagg tcagagcctt ttttaacgga ctctgttttt
     5041 tggtgcggat aatgattccc ttgcgaccct tcccgtgggt gatagtgaag tcaatctccc
     5101 tggggctttc gttacgcagt aactgagcaa tacaacgcgg ctcattcata atcacaaccc
     5161 catccacaaa agccatgcat cacgctgttc aaccggtcgg ttataaaacg cctcacgtac
     5221 agcgcgatta aactcaggaa tgaaaaccca cttctcaccg acacgagcgt tcggcttact
     5281 tggatcacga agctcaataa ctggcaattt attctctttt accatcttga ctacagccgt
     5341 ttctggctta ccaagtaact ctgcaaactt aaccgtatgt accgcatcaa tagggtactg
     5401 aatcacatag tcattgactt ccattgatta gccctttttg ctttcgtgtt acccttatta
     5461 gatccagtcc cttctaggtc gcccctgtcc tttctaggga ctggctaaca cactcaaaag
     5521 gtcaccaata cacaaccttt tgacgggaat ataagtcacc aataggttac tgtcaaatgc
     5581 agacattcga aaaactgaaa gcgattagga aagcagaagg cttaacacag gctaaattca
     5641 gcgaaattag cgggatagct ctaggaacag tcaaaaatta cgaaagtggg cataaagacc
     5701 caggtctcag catcgtgatg cgagtcacaa atacaccttt atttaaaaaa tatacgctct
     5761 ggttaatgac tggtgatacg tcaccacaag ctggtcagat cgcgccggct ctcgcacaca
     5821 ttgggcaaaa accaacagaa tcagaccact ccgggaaaca gactggttaa cactctataa
     5881 acattacatt ttcaccattt gttaccaaga tggtgaatac agcgtcagag ggctttctta
     5941 tgtcaattaa gaagctcgat gatggacgct atgaagtgga cattagacct cgcggtcgcg
     6001 acggaaaacg catccgcagg aagtttgaaa gaaaagctga ggctgtagca tttgagcgat
     6061 acacaatcgc caatgccagt cagaaagaat gggcaggcca gcgagcagac cgccgaactt
     6121 tgacagaatt gctcgacatc tggtggaaat atcacgggca aaaccacgag catgggacaa
     6181 aagagtttaa tcatctactc aaaaccatca gcggtatagg tgatataccg gtgagccgga
     6241 tgaacaaaag ggctttgatg gattatcgct ccatgcgact gcgtgatggc atcagtgctg
     6301 caacgataaa tcgtgacata taccgattat ccggcatgtt cacaaaatta attcaactgg
     6361 atgaattttc cgggcaacac ccaattcacg gactgccgcc actgacggag gccaaccctg
     6421 aaatgacgtt cctggaaaaa gcagaaatcg aaaaactgtt aaatgttttg gctggtgatg
     6481 acttacttgt cgcgctttta tgtctgagca ctggaggaag atggacggaa gttgccacgc
     6541 taaaaccagc acagattaca aattgcaggg ttaccttcct gaaaaccaaa aacggtaaaa
     6601 agcgaaccgt gccgatttct gaggaactgg agaaaaaagt taaagaggag gccagtgcca
     6661 aattattcaa agttgattat gagaagtttt gcgggatttt acgcagagtg aaacctgata
     6721 taccacccaa tcaggcaacc catatcctgc gacatacatt cgcaagtcat ttcatgatga
     6781 atgggggcaa cataattgca ctgcaacaga ttctggggca tgcgagcatt cagcagacga
     6841 tggcctatgc gcaccttgcg cctgattacc tgcaaaacgc agtagcgtta aacccactaa
     6901 agggtggttt aacgattcga taattattca ggcaacatag ttcgtttatt atcaactagg
     6961 acgataagca catcttgcga tgtaacatcg tcctgataga cgtcaaagct taatccatca
     7021 acctgtgctt tctcggcagg tatgaactta agcgaccagt tgtaaccaca gaggatggtc
     7081 ataacacctt taaaaagtga gatgaccagt ttaatgcgtt tacacagagg aatttttgaa
     7141 gagatgacaa atagttccat aagaaaaatc cattgcaggg ccagtggacg caatattgct
     7201 ggtttagcca aagagcttga cactgctgcc atagcatatc aacgagggcc aatatgtttt
     7261 ggtgccggca caaccgaata cttaaacata gttcttccat ttgctactgc agcattcggc
     7321 gctgtcgctg gggtctttgg tgcatatcta caacgaggca aaaagctacg cgttgttttt
     7381 gatgatggaa aagtaaaaga gcttgatgcg actaactaca ctcctgacga attggttagt
     7441 gcagtacaaa aaatcaagga aatcgacctg ctcgactgaa gcatcagatc cttgccgagt
     7501 gtccacattg tgtccacact cgaagaactt tgtagccctt ccagtccctt ataggttttc
     7561 ttaagttgct gttttcttaa gggaaccaat gtaagtgatt gataaaaaaa acccccacat
     7621 catgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta ctactgggaa
     7681 cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg cgtcacgtca
     7741 cgcaactgct ccattcgttg ttgatgtttc tcatttaaaa ccgcttgctg ctccggcgtt
     7801 aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc aatttgctca
     7861 ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca
     7921 aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt
     7981 tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc gtcgaacata
     8041 tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc gcctgaaccg
     8101 acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac ggcagcggta
     8161 actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata gagagtttac
     8221 gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat ggattagcga
     8281 cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa tcctgttagt
     8341 tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg aaggcttcaa
     8401 cgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca gcattgattt
     8461 acttttgctt gatgtaatga tgccgaagaa aaatggtatc gacacattaa aagcacttcg
     8521 ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagtgaac ttgatcgcgt
     8581 tctcggcctt gagctgggcg cagatgacta tctcccgaaa ccgtttaatg atcgtgagct
     8641 ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa
     8701 cgacaacggt tcaccgacac tggaagttga tgccttagtg ctaaatccag gccgtcagga
     8761 agccagcttc gacgggcaaa cgctggagtt aaccggtact gagtttaccc tgctctattt
     8821 gctggcacag catctgggtc aggtagtttc ccgtgaacat ttaagccagg aagtgctggg
     8881 caaacgcctg acgcctttcg accgcgctat tgatatgcac atttccaacc tgcgtcgtaa
     8941 actgccggat cgtaaagatg gtcacccgtg gtttaaaacc ttgcgtggtc gcggctatct
     9001 gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat cttctggctg
     9061 acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc acgccagatg
     9121 accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca tgtcgaagcg
     9181 gagctggcga acgatccgcc caacgattta atgtggtggc ggcgtctgtt ccgggcgatt
     9241 gataagtggg caccgccagg acagcgtttg ttattggtga ccaccgaggg ccgcgtgatc
     9301 ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc cgataacgcc
     9361 gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt ctcagtgcgt
     9421 gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc tcaatccgat
     9481 ttcattaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat gttggtcagt
     9541 acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac
     9601 gctgccgatg aagttgccca gggaaactta cgccagcacc cggaactgga agcggggcca
     9661 caggaattcc ttgccgcagg tgccagtttt aaccagatgg tcaccgcgct ggagcgcatg
     9721 atgacctctc agcagcgtct actttctgat atctctcacg agctgcgcac cccgctgacg
     9781 cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa ggaactggag
     9841 cgtattgaaa ccgaagcgca acgtctggac agcatgatca acgatctgtt ggtaatgtca
     9901 cgtaatcagc agaaaaatgc gctggttagc gagaccatca aagccaacca gttgtggagt
     9961 gaagtgctgg ataacgcggc gttcgaagcc gagcagatgg gcaagtcgtt gacggttaac
    10021 ttcccgcctg ggccgtggcc gctgtatggc aacccgaacg ctctggaaag tgcgctggaa
    10081 aacattgttc gtaatgccct gcgttattcc aatacgaaga ttgaagtagg cttcgcggta
    10141 gataaagacg gcatcaccat tacggtggat gacgatggtc ctggcgttag cccggaagat
    10201 cgcgagcaga ttttccgccc gttctatcgt accgatgaag cacgcgatcg tgaatctggc
    10261 ggtacaggtt tggggctggc gattgttgaa accgccatcc agcagcatcg tggctgggtg
    10321 aaggcagaag acagcccgct tggcggttta cggctggtga tttggttgcc gctgtataag
    10381 cggagttaaa ctccgcattg taggcaggat aaggcgttta cgccgcatcc ggcatttgag
    10441 caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt
    10501 ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc
    10561 cgcgtccagc ttttcgataa cccggcggcg gagagtaaac ggtgatactg ctcgtcatca
    10621 aacggcatat gccatgcgat ggcggcagcc tcctgcacgg tcacatcact gacacgggaa
    10681 accaactcca gcggcgcatc agcagatacc aaccctggcg caatcacgct atacaaccac
    10741 cccactttac ccgtgttttg catcaactgc gctatgtcgc tgatatcaaa atggtaattg
    10801 agtttatagc aaggggagcg tggctggctg acctgaatta atgcctctcc ccagcggaaa
    10861 atatcgccaa tataaacatt actttccgtg aggccgtcgg ttgagagatt ttcgccaaac
    10921 gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc gcgcccagta gagatagtgc
    10981 tcacgaggat aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc
    11041 tcgtcacctt ccagccccag ctccgtcagc atcaactcac catcaacctg gattttggca
    11101 attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga
    11161 tatcgcatct gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc
    11221 aggcgtgctg ttttcgcggt atgtatccag gcaggagctc aatagcaaac cggacgaacg
    11281 ttagttcatc cggctgtggg gattaaggaa gcaggtttat tttaccgtgc ctaaaatttc
    11341 gattttcgcc actctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag
    11401 aatcgaagtc acaatgaccg ccgtcgcaac cagcgaagtt gctgccggag ccatcggttt
    11461 aaacgcaggc accatttcag caatcagcac aggtgtcgct accgcggccc ctgcggaact
    11521 ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg cgataatcaa
    11581 cggaataccg gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg
    11641 ggcaattaca gtcaaatcaa tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt
    11701 ctgcaccgct ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa gggcaaagcc
    11761 caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaatgagg caatcccggc
    11821 agtgcccaga ataatcatcg tcatgagcgg cccggactcc aatgacatca acacaaatgc
    11881 cccagcttct tcttttgtgc cgtactgttg catgatggaa gcgtaaagtc cgccgttggt
    11941 catatccatc gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac
    12001 accatgttcc ggaataatgc gtgaggcaat tgccgcaacc acccacgcga cggcaatttt
    12061 tgttaccacc agcgtaccgg atttacgtaa taccgttccc gtcgcgctta attttattga
    12121 cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt
    12181 ggtgaatgat ccaaaatatt tccccgcctc cggcgagaag gtgtggcaca gtgcgccaag
    12241 gaatagcggg acgagcatca tcccccccgg gattttctca atcgagcgtt ttatctgcat
    12301 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaac ggagcatcag
    12361 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    12421 attgaaacac tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    12481 aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat
    12541 tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcatcccagt
    12601 tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt
    12661 aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt
    12721 cacccatcag cggggagtcc tggttagcag tagaaaccac cgccagttta tcgcctttca
    12781 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt
    12841 tgaagttatc aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgtaagg
    12901 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    12961 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa
    13021 ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc attggcgttg ttgacgtagg
    13081 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    13141 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    13201 ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt
    13261 gaaaatgatt atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga
    13321 gatgtgaagc aaatcaccca cttaatgccg tgattgccag caaatcgaca acggcggcaa
    13381 caggcgaaag gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgacgcg
    13441 acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat
    13501 ttgatcggcg cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg
    13561 tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattattct gccgtgggcc
    13621 atcagcgccc tgttactacc gaatttttgg gcgtattaca gctcgtttag tctctctaca
    13681 ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa ctacggcctg
    13741 accatgcgtt atctcggcat gtcgatgggg attggtatcg ccattggtat cacgttgatt
    13801 gttggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgttgat caataccgaa
    13861 ggtggacgca tgacattgct cggcgttctg gtggcgctga ttggtgtagg gattgtgact
    13921 cgcgccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caacctgaaa
    13981 aaagggctgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc ctttgcgatg
    14041 aacgccgcaa aaccgatgca tgaagccgct gctgcactgg gcgtcgatcc actgtatgtc
    14101 gctctgccaa gctatgttgt catcatgggc ggcggcgcga tcatcaacct cggtttctgc
    14161 ttcattcgtc tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa
    14221 ccgctgatct ttcacaatgt gttactctca gcactgggcg ggttgatgtg gtatctgcaa
    14281 ttctttttct atgcctgggg ccacgcgcgt attccggcgc agtatgacta catcagctgg
    14341 atgctgcata tgagcttcta tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag
    14401 tggaacaatg caggtcgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt
    14461 gttgccgcca acatcgtcgg catgggcatg gcgaattaat ctttctgcga attgagatga
    14521 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta
    14581 tcttcaaagc cacattcggt tgaaatatca ctgattaaca ggcggctatg ctggagaaga
    14641 tattgcgcat gacacactct gacctgccgc agatattgat tgatggtcat tccagtctgc
    14701 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaattcatcc
    14761 agcgcaaagg gacttttcag gctggccgcc agccgggtaa tcagcttatc cagcaacgtt
    14821 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc
    14881 aactgcccga acagcaactc tgccatttcg ttagcaaacg gcacatgctg actactttca
    14941 tgctcaagct gaccgataat ctgccgcgcc tgtgccatcc ccatgctacc taagcgccag
    15001 tgtggctgcc ctgcgctggc gctaaatccc ggaatcgccc tctgccagtc aagattcagc
    15061 ttcagccgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag
    15121 gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga
    15181 tcgttgagta catgcagacc attaccgcgc cagacaatca ccagctcaca aaaatcatgt
    15241 gtatgttcgg caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg
    15301 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc
    15361 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta
    15421 ttgcagaaaa ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg
    15481 aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcag cgataggcga tgtcagtaac
    15541 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta
    15601 acgctgaggc gtcagtcccg ttttctgctt aagctgccga tgtagcgtac gcagcgaaag
    15661 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca
    15721 ggccagaagc aagttgagac gtgacgcgct gttttccagg ttctcctgca aactgctttt
    15781 acgcagcaag agcagtaatt gcataaataa gatctcgcga ctggcggtcg agggtaaatc
    15841 attttcctct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaacacgct
    15901 gtggttaacg cgccagtgag acggatactg cccatcctgc tcttgtggca gcaactgatt
    15961 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag
    16021 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc
    16081 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac
    16141 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat
    16201 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc
    16261 ctcctgatgt cgtcaacacg gcgaaatagt aaccacgagg tcaggttctt accttaaatt
    16321 ttcgacggaa aaccacgtaa aaaacgttga tttttcaaga tacagcgtga attttcagga
    16381 aatgcggtaa gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct
    16441 ttccctggtt gccaatgacc cattttcctg tcagtaacga gaaggtcgcg aagtcaggcg
    16501 ctttttagac tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg
    16561 tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat
    16621 gccgcagcct gacgctgcgt gaaatccatc gttttaacaa tgggctgcat agccagaacg
    16681 gttatgtcac ctgggatgtg gatagcctgg aaagtgccat tcgccttgga ttaaacaagg
    16741 tgtgcgagga agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg
    16801 tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca
    16861 ccaatggcct gatggcgcag gcacaacagc aactcggcaa acgcgatatt tatcagcgta
    16921 gcggcataca atttctaccc ttcaatacga tctatcagtt gcgtgcgctg acggagcaac
    16981 aacctgaact tattccacac attgctcacg ctctgctgat ggcggattac tttggttatc
    17041 gcctgaccgg cgagatgaac tgggagtaca ccaacgccac gaccacgcaa ctggtcaata
    17101 tcaatagcga cgactgggac gagtcgctac tggcgtggag cggtgccaac aaagcctggt
    17161 ttggtcgccc gacgcatccg ggtaatgtca taggtcactg gatttgcccg cagggtaatg
    17221 agattccggt ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt
    17281 taagcggttc acgcgccgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa
    17341 gccagacgcc atttaccaat gacacggcgc tggcagccaa tatcaccaac gaaggcgggg
    17401 cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgggtgc
    17461 ttcaggagcg gcaaatcaac gatcttccgg cgcttatcgc cgcgacacag gcactgccgg
    17521 cttgccgctt caccatcaat cccaatgacg atcgctttat taatcctgac gagatgtgca
    17581 gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac
    17641 tggcgcgctg tattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgaactgg
    17701 cgcagctacg cggtgaagat ttctcgcaac tgcatattgt cggcggtggc tgccagaacg
    17761 cgctgctcaa ccagttatgt gctgatgcct gcggtattcg ggtgatcgcc gggcctgctg
    17821 aagcctcgac gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg
    17881 tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc
    17941 ctgacagtga aattgcccac tatgtggcgc agattcactc tacacgacag acaaaggagc
    18001 tttgcgcatg accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc
    18061 ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca
    18121 ctgctggcag ggcgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggggg
    18181 gattcaggct actggcaatt atccgggcaa agcgcgtaat ggcagcgagc tacgtgccga
    18241 tttggaacaa gctatgcggc tgattccggg gccgaaacgg cttaatttac atgccatcta
    18301 tctggaatcc gatacgcccg tcgcgcgcga ccagatcaaa ccagagcact tcaaaaactg
    18361 ggttgaatgg gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttttcgca
    18421 tccgctaagc gccgatggct ttacgctttc ccatgccgac gacagtattc gccagttctg
    18481 gattgatcac tgcaaagcca gccgccgtgt ttcagcctat tttggcgagc aacttggcac
    18541 gccatcggtg atgaacatct ggatcccgga tggcatgaaa gatatcaccg ttgaccgtct
    18601 cgctccgcgc cagcgtttgc tggcagcact ggatgaggtg atcagcgaga agctggatcc
    18661 ggcgcaccat atcgacgccg ttgagagcaa attgtttggt attggcgcgg agagctacac
    18721 ggttggctcc aatgagtttt acatggggta tgccaccagc cgccagacgg cgctgtgcct
    18781 ggacgccggg catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct
    18841 gtatgtaccg cagttgctac tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt
    18901 agtgctgctg gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt
    18961 tgaccgggtg catatcggcc tcgacttctt tgatgcctct atcaaccgta ttgctgcgtg
    19021 ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc
    19081 tgagctgcgc aagctggaag cggcgggcga ttacagtgcg cgtctggcac tgctggaaga
    19141 gcagaaatcg ttgccgtggc aggcggtgtg ggaaatgtat tgtcagcgtc acgatacccc
    19201 agcaggtagc gaatggctgg agagcgtgcg ggcttatgaa aaagaaattt tgagccagcg
    19261 tgggtaaaca ctgccggatg cggcgcgagc gccttattcg gccgacagat cgtgcatccg
    19321 acaacaccga atttacagga acaccgaaca tgcaaaacat tactcagtcc tggtttgtcc
    19381 agggaatgat taaagccacc accgacgcct ggctgaaagg ctgggacgag cgcaacggcg
    19441 gcaacctgac gctacgcctg gatgacgccg atatcgcgcc ttatcacgac aactttcatg
    19501 cacaaccgcg ctatatcccg ctcagccagc ccatgccttt actggcaaat acaccgttta
    19561 ttgtcaccgg ctccggcaaa ttcttccgca acgtccagct tgatcctgcg gctaacttag
    19621 gcgtcgtaaa agtcgacagc gacggcgcgg gctaccacat tctctggggg ctaactcacg
    19681 aagccgttcc aacctctgaa cttccggcgc acttcctctc tcattgcgaa cgcattaaag
    19741 ccaccaacgg taaagatcgg gtgatcatgc actgccatgc caccaacctg atcgccctta
    19801 cctacgtact ggaaaacgac accgcggtct tcactcgcca actgtgggaa ggcagcaccg
    19861 aatgtctggt agtgttcccg gatggcgtcg gcattttgcc gtggatggtg cccggcacgg
    19921 acgaaatcgg ccaggcgacc gcacaggaaa tgcaaaaaca ttcgctggtg ttgtggccct
    19981 tccacggcgt cttcggcagc ggtccgacac tggatgaaac attcggttta atcgacaccg
    20041 cagaaaaatc agcacaagta ttagtgaagg tttattcgat gggcggcatg aaacagacca
    20101 tcagtcgtga agagttgata gcactcggcc agcgtttcgg cgttacacca ctcgccagtg
    20161 cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag gttaaccccg
    20221 acgcccacga agagtatcag cgtcggcata atccaatctg gccggaactg gaagcagtgt
    20281 tgaaatctca cggtgcgcat aactacgcca tttacctcga caaagcgcgt aatctgctgt
    20341 ttgccacggt agagattgaa tctgaagaac ggtggaatgc ggttgccagc actgaaatct
    20401 gccaacgttg gtggaaatat atgaccgacg ttatgccagc taacccggat aacagcccag
    20461 tcagtagcga gctgcaagaa gtgttttacc tgccctaatg agttgcggtg ccggaaaata
    20521 tccagcaccg cgccttcatc agaagaagta tttaaagcga acgcgtgcgc cgtaacggtc
    20581 atcagtatca tcaccgtgtt ttttatcacc gtcgctatcc agaatggagt aatacgtccc
    20641 gaggtaaata ttaaagttct ccatatccat cacattagcg aactgataag acgcatgaat
    20701 ggtgtgaatg gtgtacgtcc cttcatcatc aatccagcag tcgttatcac aaactacacc
    20761 gctgaattca tcaatcttat tgtgcgcgta gatataaccc agctcgaaac gtttccacag
    20821 cgcgttaatc ccggcagtga aatctttctc attattagca tctaaatagg cggtattaag
    20881 attaaccacg acgccatttt ccggatccgt tttcaggcca ttccaggtca tgctcatacc
    20941 atagccggta cgatcggact gatcgacaaa attacccttg ctatcggtat aaccataagc
    21001 attatttacc acgttcgctt ccatcgctgc ggaaacggta aattcttccg tcggcgacca
    21061 ggcaattacc ggacgcagat aagcaacatt tttctgctgc tccatatcgc gtccatgata
    21121 attaccgtca ttatataaag atgttccgtc ttcaagtaac gtgtttaatt caaaatacca
    21181 gttatcaagt tgtttgctga cgagaaaatt accgccagcg ttagagcgcc cgcggccctc
    21241 tttcatcata tagatataac cgctgccatc gtcataaaga tcgttcgcag tattaccgga
    21301 atgttcaaca aaggtatcct gattcagcgg gaacatatcg taggcttcga aacggcctac
    21361 tttgaccttc cagtcgtttt ctttgccaaa gaagaatacc gcatcatcga tatttactga
    21421 accgtgcatg tcccccagcg gttgcgcgga gaacccggcg aaataaccat tatccagttt
    21481 tcgcatacca tcaaaaccta acagaatacg accattgaga ttccactgtt cattagtggg
    21541 atcgctattc acatcagcgt tggtcattgc cagcaggcca tgattacttt ctgcatccat
    21601 attaaattct acgtcgccgt agatttttaa atcgccataa cctgagagca aaagattagg
    21661 tgaggtggaa tttttctttg cagattccgc cgttgcctgt ccatttaccg gcgtattgcc
    21721 ctgggcagcc ttaatttcgc ggatctcagc ggcttgttgt gtctgcagct gtttaacctg
    21781 gctctctgct tttgctgccc gcatttctgc cgcttccagg cgttcttcca gcaatgccat
    21841 gcgttgttcc agcgttaaat gctgtgtttt tgccattccg gtactacatg ccaataataa
    21901 accgatggct aaagataatg tcgatttttt cattatttac gttccctgaa ataaaagaga
    21961 ccctacgctt tccaaaagga atacgtatat agagaaatat aaggtgtgca ctttcgaatt
    22021 ttcaatccaa cttcagcagg ccgatggata gtatcccgct gcataatatg agtaaatccg
    22081 ctgatttcct ggaacgtgag gaatataaca atagtgccgt ttttaaccaa atatcgtaaa
    22141 aaatattaga tataattcac gctgactcat aagccacaaa tataaataac cttgctgcaa
    22201 aacatgtgcg acagttaaac attcacaaca tcattaaata atgatttctt attcactgca
    22261 tttattttgc ccatcaatat gtgccttact ccgcaaaatt tatccttcac tctgccttat
    22321 attgtcacga cttcacgaca tatccgcgct catcacaaaa ccgctccgcc gttacaatta
    22381 actataatat tttttcatgg aatccaataa tggcagcact tactgcaagc tgtattgacc
    22441 tgaatattca gggcaatggc acttattccg ttctgaagca gttggcgaca atggcgttac
    22501 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac tctgctgctg cgcgaaaaaa
    22561 tgcactccac cggttttggt tccggcatcg ccgtgccgca cggtaaaagc gcctgcgtta
    22621 aacaaccgtt cgtgttattc gcccgcaaag cgcaggccat tgactggaaa gccagcgacg
    22681 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagtggcgaa gaggatcagg
    22741 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatc aaccaactgc
    22801 gacagggcga cgccgaccag gtgcttgctt tgttaaatca aaccctcagc tcataaggaa
    22861 gtggcgatgg agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct
    22921 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg tcataccatt
    22981 aaagttgaaa ctcaaggatc aagcggcatc gaaaaccgct tatccagcga agagattgcc
    23041 gctgccgatt acgtgattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcga
    23101 tttgccggga aaaaggttta tgaaattgcc atctcccagg cgctgaaaaa tatcgaccat
    23161 attttcagtg aattaccgac aaactcacaa ttttttgccg cagatagcgg cgtgaagctc
    23221 ggcaaacagg aagtgcaaag tggcagcgta atgagccacc tgatggcggg cgtctctgcg
    23281 gcgctgccgt ttgtcatcgg tggcggtatt ctggtggcgc ttgccaacat gctggtgcag
    23341 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtggttgaa
    23401 tccatcggtt atctcggctt cacctttatg atccccatca tgggcgctta tattgcctcg
    23461 tcgattgccg ataaacccgc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac
    23521 aaagccctgc tcggcaccca gtctggcgcg ggcttcctcg gtgcggtggt gcttgggctg
    23581 gcgattggct atttcgtctt ctggttccgt aaagtgcgcc ttggcaaagc attacaaccg
    23641 ctgctcggtt ccatgctgat cccgttcgtt actctgctgg tttttggcgt actgacttac
    23701 tacgttatcg gaccggtgat gtccgacctc atgggcgggt tgctgcactt cctgaacacc
    23761 attcctccgt caatgaagtt tgcggcggcg tttctggtcg gtgcaatgct ggcgttcgat
    23821 atgggcggcc ctatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac
    23881 atttacgact ggtacgccat cgtcggcgtt gtcgcgctga tgccgcctgt cgcggcgggt
    23941 cttgcgacgt tcattgcgcc caagctgttt actcaacagg aaaaagaagc cgccagtagc
    24001 gccattgtgg tcggtgccac ggtcgcgacc gaaccggcta ttccttacgc tctggcggca
    24061 ccactgccaa tgattaccgc caatacgctg gcgggcggca ttaccggcgt gctggtgatc
    24121 gctttcggaa tcaaacgact ggcacctggt ttgggtatct ttgacccgct gatcggcctg
    24181 atgtcgccgg tgggttcgtt ttatctggtg ctggcgattg gtctcgcgct gaacatttca
    24241 ttcattatcg tcctgaaagg attatggtta cgccgcaaag cgaaagccgc gcaacaggag
    24301 cttgcccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg ttagcggcga
    24361 tgaacaggaa gttcgcgaca ttctgatcaa cacgctggaa ccttgcgtta atgagatcac
    24421 ctttgatggt ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt
    24481 tgtcgggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
    24541 gcgttttacc accattggcg gctggtggaa tcagtcgata ctcaaccacc gggtaaccat
    24601 acgcacacac aagggattta aaatccctgg tgtgattggt tccgtcgcgc ctcatgcgtt
    24661 aacggaaaag caaaagcaac aaccgctgtc atttgatgag atgttcattg atattggcgc
    24721 gaacagtcgc gaagaagcgg aaaaacgcgg cgtggaaatt ggtaatttta ttagcccgga
    24781 agccaatttt gcctgctggg gcgaagataa agtggtcggc aaggcgttgg ataaccgcat
    24841 cggctgcgca atgatggctg agctattgca gacggtgaat aatcccgaaa ttacgctgta
    24901 tggcgttggc agtgtggaag aagaagttgg cctgcgcggg gcgcaaacct cggctgaaca
    24961 cattaaaccg gatgttgtga tcgtgctgga taccgccgtc gcaggcgatg ttccgggcat
    25021 tgataacatt aaatacccgc taaaactggg ccaggggccg gggctgatgc tgtttgataa
    25081 acgctacttc cccaaccaga agctggtggt ggcgttaaaa agctgtgcca cacataacga
    25141 tttaccgctg caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt
    25201 gatgggcggc gggcgtccgg ttgtcgcgct gtgcctgcca acgcgttatc tgcacgccaa
    25261 cagcggcatg atttcaaaag ccgattacga tgctctgctc acgctgatac ggggttttct
    25321 gacgacctta actgcggaga aaatcaacgc gtttagccag ttccgtcagg tggattaatg
    25381 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggc
    25441 gagctggcac aacagactgg cgtttcgggc aggaccatcc tgcgcgacat tgactatctc
    25501 aactttaccc ttaacggcaa agcccgcatt tccgccagtg gcaacgcggg ctatcagctg
    25561 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
    25621 ctgctggcgc tgctattact gaataccttc actccccgtg cgcaactcgc ctcggcgctt
    25681 aatttgccag aaacgtgggt agcagagcgc ctgccccgcc tgaaacagcg ctatgaacgc
    25741 ggtttttgtc tcgctagtcg ccctggtttg ggccatttca ttgatgagac agaagagaaa
    25801 cgcattattt tgctggcgaa tcttttacgc aaagatccgt ttttaattcc tctggcgggc
    25861 gtaacacgag acaaccttca gcaattatcc acggtctgcg acaaccaaca ccgctggccg
    25921 ctgatgcagg gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
    25981 ctgaccgatg agtggccgca atatcccggt agcgagataa aacagatcgt tgaatatagc
    26041 ggcctgtttc ttggtgataa cgctgtaaga acgttgacgg ggttgataga aaaacagcat
    26101 caacaggcgc aggttatttg tgtcgataat gtgctggcat tgctgcaaag ggtgccgggc
    26161 attgcgtcat tgaatattat tgatacgcaa ctggttgaga atattaccgg gcatttatta
    26221 cgttgccttg ccgcaccagt atggattgct gagcaccgcc aaagcagcat gaataacctg
    26281 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
    26341 cagctcgata ttcccctttt cgacagcgat ctgatcggtt tgtattttgc ctgtgcgctg
    26401 gaacgacatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
    26461 actattaatc agctcgccat tgagcgcgat gtcctgaatt gtcgggtgat tattgcccgc
    26521 agcctgagcg atattaccgc cattagccag gagattacgc cgttattgat cgtcaataac
    26581 agccactatt tgctggatga cgcggtaaaa aatagtatta ccatcaaaaa tatcattacc
    26641 gccgccggga ttgagcaaat taaacatttt cttgccacgg catttattcg ccagcagccg
    26701 gagcgttttt tctctgcccc cggaagtttt catttttcga atgtacgcgg tgaaaactgg
    26761 caacatatta cccagcaaat atgtgcacaa ttagtgacgc aacaccacat taccgccgat
    26821 gaagcacaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgtctc
    26881 gccatcccac attgctggag cgaacaggaa cgccgttttc gtggattttt tattaccctc
    26941 gcccaaccgg ttgaggtgaa taacgaagta gttaaccatg tgctgatcgc ctgtgccgcc
    27001 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
    27061 ccggcagagg ttattgccgg gttaaaagga tatgaggcat ttatggagtt acttcacaag
    27121 gggtgaactg tgtcggatgc gccgcatccg acactgcatt tatcaccaaa atcaaaaacg
    27181 tttaacgcag gctattatag tcatcgacca tttcgttgta ctcgcgtaac gctcgcggaa
    27241 atgagtcgtc gaccatccag ctggtgtttg catcctgaag ttgctcttta tcccagtcag
    27301 agtacggaac tttatctctg acgcggcgaa catatttttt agcctcgagt tgatactggt
    27361 ctgccgaatt aatgaacgag aaattcatac cgcctttatc cgcttctttg gcctgcgcca
    27421 ccagcgtttc cagttcagag actttcttca ttgccgcatc aacatcaaac ttctcttgcg
    27481 aaatcaggtt attaatttgt ttcgctgaaa tcatgactgc cagagagtag tagtggaatg
    27541 ttttcccttc tctttgttca atatttttca gttccgcaag ctggcgctta tcgttaattt
    27601 cctgaatcgc cgcatgataa gattctgcta caggttcaaa ggcttcaaga ttttttaaga
    27661 atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt
    27721 aatatttatc catttcgtta atggtattac ccaacgcc
//
LOCUS       DARNVM010000050        27568 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.050, whole genome shotgun sequence.
ACCESSION   DARNVM010000050 DARNVM010000000
VERSION     DARNVM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.050"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            77..2500
                     /gene="pgaA"
                     /locus_tag="SI621_001928"
     CDS             77..2500
                     /gene="pgaA"
                     /locus_tag="SI621_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HEI4064655.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAANNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLESQNKDYQRGQILTLADAGHYDSALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHEDELRAMTGSLPENALTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTCSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIIRDWLAGVEWRSRNIWLEAEYTERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2509..4527
                     /gene="pgaB"
                     /locus_tag="SI621_001929"
     CDS             2509..4527
                     /gene="pgaB"
                     /locus_tag="SI621_001929"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HEI4064656.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4520..5845
                     /gene="pgaC"
                     /locus_tag="SI621_001930"
     CDS             4520..5845
                     /gene="pgaC"
                     /locus_tag="SI621_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HEI4064657.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWAIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5847..6260
                     /gene="pgaD"
                     /locus_tag="SI621_001931"
     CDS             5847..6260
                     /gene="pgaD"
                     /locus_tag="SI621_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HEI4064658.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6310..7098)
                     /gene="phoH"
                     /locus_tag="SI621_001932"
     CDS             complement(6310..7098)
                     /gene="phoH"
                     /locus_tag="SI621_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HEI4064659.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7718..8989)
                     /gene="efeB"
                     /locus_tag="SI621_001933"
     CDS             complement(7718..8989)
                     /gene="efeB"
                     /locus_tag="SI621_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEI4064660.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIASDNLTITLSVGHSLFDERFGLTPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQQVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(8995..10122)
                     /gene="efeO"
                     /locus_tag="SI621_001934"
     CDS             complement(8995..10122)
                     /gene="efeO"
                     /locus_tag="SI621_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEI4064661.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10180..11010)
                     /locus_tag="SI621_001935"
     CDS             complement(10180..11010)
                     /locus_tag="SI621_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEI4064662.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11664..13172)
                     /gene="putP"
                     /locus_tag="SI621_001936"
     CDS             complement(11664..13172)
                     /gene="putP"
                     /locus_tag="SI621_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEI4064663.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13331..13540)
                     /locus_tag="SI621_001937"
     CDS             complement(13331..13540)
                     /locus_tag="SI621_001937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064664.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13595..17557
                     /gene="putA"
                     /locus_tag="SI621_001938"
     CDS             13595..17557
                     /gene="putA"
                     /locus_tag="SI621_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEI4064665.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPIAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDTLHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17597..18235)
                     /gene="rutR"
                     /locus_tag="SI621_001939"
     CDS             complement(17597..18235)
                     /gene="rutR"
                     /locus_tag="SI621_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HEI4064666.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18523..19614
                     /gene="rutA"
                     /locus_tag="SI621_001940"
     CDS             18523..19614
                     /gene="rutA"
                     /locus_tag="SI621_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEI4064667.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19614..20306
                     /gene="rutB"
                     /locus_tag="SI621_001941"
     CDS             19614..20306
                     /gene="rutB"
                     /locus_tag="SI621_001941"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HEI4064668.1"
                     /translation="MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20318..20704
                     /gene="rutC"
                     /locus_tag="SI621_001942"
     CDS             20318..20704
                     /gene="rutC"
                     /locus_tag="SI621_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEI4064669.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20712..21512
                     /gene="rutD"
                     /locus_tag="SI621_001943"
     CDS             20712..21512
                     /gene="rutD"
                     /locus_tag="SI621_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEI4064670.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVIC
                     YDQRGTGNNPDTLEEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASLTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21522..22112
                     /locus_tag="SI621_001944"
     CDS             21522..22112
                     /locus_tag="SI621_001944"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEI4064671.1"
                     /translation="MNEAISPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSIQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22123..22617
                     /gene="rutF"
                     /locus_tag="SI621_001945"
     CDS             22123..22617
                     /gene="rutF"
                     /locus_tag="SI621_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HEI4064672.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22638..23966
                     /gene="rutG"
                     /locus_tag="SI621_001946"
     CDS             22638..23966
                     /gene="rutG"
                     /locus_tag="SI621_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HEI4064673.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24049..24222)
                     /locus_tag="SI621_001947"
     CDS             complement(24049..24222)
                     /locus_tag="SI621_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEI4064674.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24595..25191
                     /gene="wrbA"
                     /locus_tag="SI621_001948"
     CDS             24595..25191
                     /gene="wrbA"
                     /locus_tag="SI621_001948"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEI4064675.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25212..25439
                     /locus_tag="SI621_001949"
     CDS             25212..25439
                     /locus_tag="SI621_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HEI4064676.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25477..26718)
                     /gene="agp"
                     /locus_tag="SI621_001950"
     CDS             complement(25477..26718)
                     /gene="agp"
                     /locus_tag="SI621_001950"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HEI4064677.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27011..>27568)
                     /locus_tag="SI621_001951"
     CDS             complement(27011..>27568)
                     /locus_tag="SI621_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064678.1"
                     /translation="TQKEVYEEFIKIVDKPTIEALVDAAFCDLREDVKTLFGVDLQSD
                     NKIQGVGSLMSWSFLFFKKEFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIK
                     AVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDREYGYLINTGNHYDVYLPPE
                     LFAQAYKLNNKERNAQIDFLTRYAI"
BASE COUNT         6754 a         7126 c         6971 g         6717 t
ORIGIN      
        1 aaaaaaatat ctttcgtttc agttacctgt aattagatac agagagagat tttagcaata
       61 catggagtaa tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa ccaaatgggc
      121 tttgaaaatt cttactgccg catttttagc agcgagtccc gcggcgaaga gtgctgctaa
      181 taatgcctat gatgcattga ttatcgaagc tcgcaaggga aatactcagc cagctttgtt
      241 atggtttgca caaaaatcag cactcagtaa taaccaaatt gctgactggt tacagattgc
      301 cttatgggcc gggcaagata aacaggttat taccgtttac aaccgctacc gtcatcagca
      361 attaccagcg cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc aacaatggca
      421 aaactcgctt acactgtggc aaaaggcact ctctctggag tcgcaaaata aggattatca
      481 acggggacaa attttaaccc tggcagatgc gggtcactat gattctgcgc tggttaaact
      541 taagcagctt aactctggag caccggacaa agctaattta ctcgcagaag cctatatcta
      601 taaactggcg gggcggcatg aggatgaatt acgggcgatg acagggtcat tacctgaaaa
      661 tgccttaacg caacaatatc ccacagaata cgtgcaggca ttacggaata atcaacttgc
      721 tgccgcgatt gacgatgcca atttaacgcc agatattcgc gctgatattc atgccgaact
      781 ggtcagactg tcatttatgc ctacgtgcag tgaaagtgaa cgttatgcca ttgccgatcg
      841 ggccctcgcc caatacgctg cattagaaat tctgtggcat gataaccctg accgcactgc
      901 ccagtaccag cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg atcgttataa
      961 agacgttatt tctcactatc agcgtttaaa aaagacgggg caaattattc cgccctgggc
     1021 gcaatattgg gttgcgtcgg cttatctcaa agaacatcag ccaaaaaaag cacagtcaat
     1081 aatgacagag ctcttttatc acaaggagac cattgccccg gatttatccg atgaagaact
     1141 tgcggatctc ttttacagcc acctggagag tgaaaattat cccggcgcgc taactgtcac
     1201 ccaacatacc attaatactt cgccgccttt ccttcggtta atgggcacgc ctacgagcat
     1261 cccgaatgat acctggttac aggggcattc gtttctctca accgtagcaa aatatagtaa
     1321 tgatcttcct caggctgaaa tgacagccag agagcttgct tataacgcac caggaaatca
     1381 gggactgcgc attgattacg cgagtgtgtt acaagcccgc ggttggcctc gtgcggcaga
     1441 aaatgaatta aaaaaagcag aagtgatcga gccacgtaat attaatctgg aggttgaaca
     1501 agcctggaca gcattaacgt tacaagaatg gcagcaggca gcggtcttaa cgcacgatgt
     1561 tgtcgaacgt gaaccgcaag atcccggcgt tgtacgttta aaacgtgcgg ttgatgtaca
     1621 taatcttgca gagcttcgta tcgctggctc aacaggaatt gatgccgaag gtccggatag
     1681 tggcaaacat gatgtcgact taaccaccat cgtttattca ccaccgctga atgataactg
     1741 gcgcggtttt gctggattcg gttatgccga tggacaattt agcgaaggaa aagggattat
     1801 tcgcgactgg cttgcgggtg ttgagtggcg gtcacgtaat atctggcttg aggcggagta
     1861 caccgaacgc tttttcaatc atgagcataa acccggcgcg cgcctgtctg gctggtatga
     1921 ttttaatgat aactggcgta ttggttcgca actggaacgc ctctctcacc gcgttccatt
     1981 acgggcaatg aaaaatggtg ttacaggcaa cagtgcacag gcttatgttc gctggtatca
     2041 aaatgagcgg cgtaagtacg gtgtctcctg ggctttcact gatttttctg acagtaatca
     2101 gcgtcatgaa gtctcacttg agggtcagga acgcatctgg tcttcaccat atttgattgt
     2161 cgatttccta cccaatctgt attacgaaca aaatacagaa cacgacactc catactacaa
     2221 ccctataaaa acgttcgata ttgttccggc atttgaggca agtcatttgt tatggcgtag
     2281 ctatgaaaat agctgggagc aaatcttcag cgcaggtgtt ggtgcctcct ggcaaaaaca
     2341 ttatggcacg gatgtcgtca cccaactcgg ctacgggcaa cgcattagct ggaatgacgt
     2401 gattgatgct ggcgcaacgc tacgctggga aaaacgacct tatgacggtg acagagaaca
     2461 caacttatac gttgaattcg atatgacatt cagattttaa ggataaatat gttacgtaat
     2521 ggaaataaat atctcctgat gctggtgagt ataattatgc tcactgcgtg cattagccag
     2581 tcaagaacat catttatacc gccacaggat cgcgaatctt tactcgccga gcaaccgtgg
     2641 ccgcataatg gttttgtggc gatttcatgg cataacgttg aagacgaagc tgccgaccag
     2701 cgttttatgt cagtgcggac atcagcactg cgtgaacaat ttgcctggct gcgcgagaac
     2761 ggttatcaac cggtcagtat tgctcaaatt cgtgaagcac atcaaggagg aaagccgcta
     2821 ccggaaaaag cagtagtgct aacttttgat gacggctacc agagttttta tacccgcgtc
     2881 ttcccaattc ttcaggcctt ccagtggcct gctgtatggg cccccgtcgg cagttgggtc
     2941 gatacgccag cggatgaaca agtaaaattt ggcgatgaga tggtcgatcg agaatatttt
     3001 gccacgtggc aacaagtgcg agaagttgcg cgttcccggc tcgttgaggt cgcttctcat
     3061 acatggaatt ctcactacgg tattcaggct aatgccaccg gcagcttatt gcctgtatat
     3121 gtaaatcgtg catattttac tgaccacgca cggtatgaaa ccgcagcaga ataccgggaa
     3181 agaattcgtc tggatgctgt aaaaatgacg gaatacctgc gtacaaaggc taaggtaaat
     3241 ccacacgttt ttgtttggcc ttatggcgaa gcgaatggca tagcgataga ggaattaaaa
     3301 aaactcggtt atgacatgtt cttcaccctt gaatcaggtt tggcaaatgc gtcacaattg
     3361 gattccattc cgcgagtatt aatcgccaat aatccctcat taaaagagtt tgcccagcaa
     3421 attattaccg tacaggaaaa atcaccacaa cggataatgc atatcgatct tgattacgtt
     3481 tatgacgaaa accgccagca aatggatcgc aatattgatg tgctaattca gcgggtgaaa
     3541 gatatgcaaa tatcaaccgt gtatttgcag gcatttgctg atcccgatgg tgatgggctg
     3601 gtcaaagagg tctggtttcc aaatcgtttg ctaccaatga aagcagatat ttttagtcgg
     3661 gttgcctggc aattacgtac ccgctcaggt gtaaacatct atgcgtggat gccggtatta
     3721 agctgggatt tagatcccac attaacgcga gtaaaatact taccgactgg ggagaaaaaa
     3781 gcacaaattc atcctgaaca atatcgccgt ctctctcctt tcgatgacag agtcagagca
     3841 caagttggca tgttatatga agatcttgcc ggacatgctg cttttgatgg catattgttc
     3901 cacgatgatg ctttgctttc agattatgaa gatgccagtg caccggctat cacggcttat
     3961 cagcaagcag gctttagcgg gagtctgagc gaaattcgac aaaacccgga gcaatttaaa
     4021 cagtggaccc gctttaaaag tcgtgcgtta actgacttca ctttagaact tagtgcgcgc
     4081 gtaaaagcca ttcgcggtcc acatgttaaa actgcacgaa atatttttgc acttccggta
     4141 atacaacctg aaagtgaagc ctggtttgca cagaattatg ctgatttcct aaaaagctat
     4201 gactggaccg ctattatggc tatgccttat atggaaggtg tcgcagaaaa atcggctgac
     4261 caatggttaa tacaattgac caatcaaatt aaaaacatcc ctcaggctaa agacaaatct
     4321 attttagaat tacaggcaca aaactggcag aaaaatggtc agcatcaggc tatttcttcg
     4381 caacaactcg ctcactggat gagcctatta caactgaatg gagtgaaaaa ctatggttat
     4441 tatcccgaca attttctgca taaccaacct gaaatagacg ttattcgtcc tgagttttca
     4501 acagcctggt atccgaaaaa tgattaatcg catcgtatcg ttttttatat tatgtctggt
     4561 gttatgcata cccctatgcg tagcgtactt tcactctggt gaactgatga tgaggttcgt
     4621 tttcttctgg ccgtttttta tgtccatcat gtggattgtt ggcggcgtct atttctgggt
     4681 ctatcgtgaa cgccactggc cgtggggaga aaacgcacca gctccccagt tgaaagataa
     4741 tccgtctatc tccattatca ttccctgttt taatgaggag aaaaacgttg aggaaaccat
     4801 acacgccgct ttagcacagc gttatgagaa cattgaagtt attgccgtaa atgacggttc
     4861 aacagacaaa acccgtgcca tcctggatcg catggctgca caaattcccc acctgcgggt
     4921 cattcatctg gcgcaaaacc aggggaaagc cattgcgctt aaaaccggag ctgccgcggc
     4981 gaaaagtgaa tatctggtgt gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc
     5041 atatattgtg gaaccgatgt tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg
     5101 tattcgaaca cgttctaccc tggtgggtaa aattcaggtt ggcgagtatt cctcaattat
     5161 tggtttgatc aagcgaaccc agcgtatcta tggaaacgta tttaccgttt ccggtgttat
     5221 tgccgcattt cgtcgcagcg ccctggcaga agtgggttac tggagtgacg atatgatcac
     5281 cgaagatatt gatattagct ggaagctgca gttgaatcag tgggcgattt tttacgagcc
     5341 acgggcgctg tgctggatat taatgcctga aacgttaaaa gggctgtgga aacagcgcct
     5401 gcgctgggct cagggcggtg cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa
     5461 agaaaacttt cgaatgtggc cgctgttttt tgaatactgc ctgacgacaa tatgggcctt
     5521 cacttgcctg gtcggtttca ttatttacgc agtccaactt gccggtgtac cgttaaatat
     5581 tgaattgaca catatcgctg cgacacatac tgccggaata ttattgtgta cgttatgttt
     5641 actgcaattt attgtcagcc tgatgatcga gaatcgctat gagcataatc tgacttcatc
     5701 gcttttctgg attatttggt tcccggttat tttctggatg ctgagtctgg caacgacatt
     5761 ggtatcattt acacgagtca tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc
     5821 cgatcgcggg attctgagag gttaatatga acaatttaat tattacgacc cgacaatcgc
     5881 cagtacgttt actggttgat tatgttgcca caaccatctt atggacatta tttgctttgt
     5941 tcatattctt attcgcaatg gatctgctga cgggttatta ctggcaaagc gaggccagaa
     6001 gccgacttca gttctatttt ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg
     6061 cgctgtacaa taagctacgt tttcaaaaac agcagcatca tgcagcctac caatatacgc
     6121 cgcaagaata tgctgagagc ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa
     6181 gccacaggat gagcgtacac ttcaccagcc aggggcaaat aaaaatggtg gtttcagaaa
     6241 aagcgctagt ccgggcgtaa acacccaaaa caaagcccgg ttcgcccggg ctctgcgccg
     6301 ataatacact taactgtatg catgtagcgt acgttggcaa agtgccgaac gtacgcaatc
     6361 ctctttaccg aatcggacga tcccgaccat ttcatcttct tcgaaacgtt ccagcgcgtc
     6421 acttaatccg gagcgcacgc cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat
     6481 aaccgtcacg ttctccccga ggcgggttaa aaacattttc atttgcgcgg cagtcacatt
     6541 ctgcgcctcg tcaagaatga cgactgcatt ttcaaaggta cgtccacgca tataggcgaa
     6601 cggcgcaatt tccaccttcc ctatttccgg tcgcaggcag tactgcataa aggaagcccc
     6661 taagcgccgg accagcacgt cgtagaccgg acgaaaatag ggagcaaact tttctgcgat
     6721 atcaccaggt aagaagccaa gatcttcatc ggcttgcaga actggacggg tgacgataat
     6781 cctgtcgaca tccttatgta tcagggcctc tgccgctttt gctgcgctga tccaggtttt
     6841 tccgcacccg gcttcgcccg tggcgaatat cagctgctta ctctcaatag ccttcagata
     6901 gtgcaattgc gcttcatttc gcgcgaggat gggcgaggta tcgcgactgt cgcgggccat
     6961 accaatggct tctacgccgc tcatctgcac aagcgaggtg accgattctt cttcacgctg
     7021 cttatggctg cgcgaatccc gtctcagcac acgttttgcc tcgcgacgag ctttgatcac
     7081 tgctttttgt cttcccatgg agagcacctt gagttgtttg tattcatcac acgcgccgtt
     7141 ggcagcgcga ttatgcgcac gaacatcaga gggttggctt ccttgtaagc catagtttgc
     7201 ttttggataa aatgccgaaa aacggctacg cacaccgttt gcggcgtcgg taacacgagt
     7261 aaagagagga agtgagaaaa atacagagtg acgaagttgc taccggagga gaaatctccg
     7321 cgagtggtgg agccttgctt aacaaaacat tgtccagaac aggacgttac catccgcgat
     7381 ctccatagtg actgactatc actgccggga acttccgctg ctacttaata agtacaacag
     7441 atctcgcatt cattgcaaca atatattcac ttatatttaa ctacaaaaca ccatttcagt
     7501 gacattagtt tctactggaa agatgacaga gtgatgacag tgatgaaaaa agctgtgtgc
     7561 tttcagcagg attcgcatca ggcatcagcg cacgaacgcc ggatgcggcg tgaacgcctg
     7621 atccagccta ccaaacccgc accgattgta ggcatgataa gacgcgcaag cgtcgcatca
     7681 ggcatctgcg cacgaacgcc ttatccgcct aaacacatta aacccgcaat aacgcgcttc
     7741 cgagataatc gttcgcgtcc ttcactcccg gcagcgaaaa aaaataaccg ccgccgatag
     7801 gtttaacgta ttcctccagc gcttcgccat tgagcctttt ttgtactgtc aggaagcctt
     7861 tttccagatc gtgttgatag cagacaaaca gtagccccat atccagttgt ccggagttgg
     7921 tgacgcccaa cgaataactg tagccacgac gcagcatcag gctggactca ctctctggcg
     7981 tgcggggatt cgccagccgg atatggctgt ccagcgcaat caccttcccc tccggatcgc
     8041 tggcgtaatc gggaacatca tgctcatgct gcattcccag cggcgcaccg gtttgtttgt
     8101 cgcgaccaaa aatcgtctgc tgttctttca gtggcgttct gtcccaaaac tccacccgaa
     8161 actgaatcaa ccgcaccgcc tggtagctgc cgccgattgt ccacgcaggc tcctgctgat
     8221 ctgccgtcac ccacaccact tgttgcatca acttatcatt ctggctatcg ggattggcag
     8281 tgccgtcttt gaaacccagc aaattaatcg gcgtctcttt gcctttacta cgcgctgcat
     8341 gatcggaaat aaacccttcc cgcttccagc gcacgctgag caaatccggc gtgtgtttga
     8401 tgatatcgcg cagcgcatgg atcaccgtgt cctgggtgtt ggcgcaaatt tgtagcaaca
     8461 catcaccatg acataacgcc gcatccagcg agtcgttggg gaaacgcgtc attttctgca
     8521 actttttcgg catctgcggt gtaaggccaa accgctcatc aaacaatgag tggcccaccg
     8581 ataacgttat ggtgagatta tcggacgcaa tgtagccgcc aagaatgccg gaatcgagtg
     8641 gaggcaggcg cggatttggc gtttctggtg ccgctccgcc ctgagtcaga aaagcaaaac
     8701 gctgagtcaa caagcgaaac aacctttcaa gatcggcttt atcgctggca agcacatcaa
     8761 acgccaccag catcattgcg gcctgttgtg gcgtcaggat ccctgcctga tgctcaccat
     8821 aaaacggctg tttctcattg cgtgcatccg gtgaaagcgt gcccggtgca ctttgcgttt
     8881 tttgtgcatg agcgaccgga caacttcccg ccagcgccag cgcaccaatc cctttcagta
     8941 aacgtcggcg tgacggttca ttcacgccgt tttcatcttc atactgcata acgtttaatc
     9001 cagccccagc acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt
     9061 cagcgcatta cggtcagcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg
     9121 gtatttcgcc agaatggtat cgaccttttt gaaattggca tcgacttttg ccagcagttc
     9181 cgggttggcc ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac
     9241 gttagcctgg aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt
     9301 gctggctgcc acttcctcaa tcagtccggc tgcaccgccg accacttttg aaggtgggaa
     9361 agccagttca ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct ggtcggcata
     9421 cttatccatc cctttagtgg tgttatcgcc aaacaatgct ttttccagac ggtggaaacc
     9481 ggtgaatttt ggatcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctacc
     9541 atccagatcg gagaacagtt cagcaatcgg ttcaatgcgc tcatagtgct ggcgcgtcgg
     9601 cgcatacagt gcttttgctt tttcgatatc gcctgcttta atagcgtcgg taaaggcttt
     9661 ggtgtcggtc accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc
     9721 accaccaaga cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac
     9781 gatcaacttc cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg
     9841 taaattcgcc gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac
     9901 catcacgcct ttgaggatct cccactccag cgccttctgg ctgtggttct gaataatgaa
     9961 ctgtgttttc ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt
    10021 cactttgacc tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc
    10081 cacgctcaac tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta
    10141 aagtatgttg taactaagca agagtttgcg tcgtatttgc tacgcagagc gagacgctgt
    10201 cgcccctgcg cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca
    10261 gacggcgact tcgctgacac ttggcgcttc ctgatagcca aaaatccctt ccatcagcgt
    10321 gccaaacagc gagtgagttg agagcaccgc actcatatcg aaggcgattt cctgaaagtg
    10381 gttccacaat ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac
    10441 gaagagaata aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc
    10501 ccagtagagc aggaagccga gcaccacggc tgtagcaaga ccgagcattg cacccagcgg
    10561 cggccagatc ccgacatctt gttgaaacgc cgccagcagg aaaaagaccg actccagccc
    10621 ctcccttgca acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg
    10681 ctgcaatgcg ctatcgactg cctgctccag ttgtactttg acgttgcgcg acactttgcg
    10741 catccagaaa accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa
    10801 cagttcctgt tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tacccaagcc
    10861 caggcacaac gcagcggcaa gcaacacgcc aatccacatc acaccaatcc atcggcctcg
    10921 ctgggtacgc ttaagatagc tggcgatcaa actgacaatc agcgcggctt caagtccttc
    10981 gcgcaacata atgagaaatg gaacaaacat gccacccacc cttaaacgtt tttctgagtc
    11041 aatctatgca aagaaaagta aatcgcagtg atagcgatta tcattacggc aaagaaaaat
    11101 acaagcggaa tgtttataaa atgatgttgg aatgtaaaca aagagaggta atgccagggg
    11161 atataaacga ttattccagg tacaaagatg ggattgttta caacgagcca tgtgttcttg
    11221 ccagatgccg cgtaaacgcc ttatccagcc gacaaaactg tccagactca atgcatcaga
    11281 gcttccatgt aaaactgata agcgggtgta tcaagcaatt tcgtccatcc gcgattatca
    11341 aatctaactt tttgcccgga acataataac agctcgggtg ttggtggcgt caaagtcgct
    11401 gaagcgttcc ccgaaggccg gggcagccca catggatgtg ggctgagggc gcgtcttaca
    11461 ggacattacc tcgcgcccga cccggtagcc gaaggggata agctgaaggt accgcgcagc
    11521 ggcgattttg ttcgccagag cccggggtgc agggggtggc ggcgattggc cgccccctgc
    11581 accattcaat ttatatggcg aggcacatta agggagcgca acccgctgct gaagccaaac
    11641 aaaaccgccg caggctaagt cccttagctt tcctgcaacc gtgacggcgg agccgaatga
    11701 tagtgcgcat cggcctcggc aaagcgtttt tgcatcgccg ctgacggcgc tttacccagc
    11761 aaactaaaca ctacaatccc gatactacca aagataaagc ccggaataat ttcgtacaga
    11821 cccagccagc cgaactgttt ccagacgata accgtcagcg caccgatgat catccccgcc
    11881 agcgcaccgt tgcgcgtcat gcgtgaccac atcaccgaga acagcaccac cggaccaaac
    11941 gccgcgccaa agcctgccca cgcgtagctc actaagccca gcacgcggtt ttccgggttt
    12001 gccgccagcg caatggccac cagcgccacc accagcacca tcacacgccc tacccacacc
    12061 agctctttct ggctggcctg tttacgcaga aacgctttgt ataaatcttc ggtaatcgca
    12121 ctggagcaca ccagcagctg gcaacttaag gttgacatta ccgccgccag aatcgccgac
    12181 agcagaatcc cggcaatcca cgggttaaac agaatttgcg ccagttcgat aaacacacgt
    12241 tcagcattct ggtttaccgc acctgctacc gacgggtgct cattaaagta agcaatacca
    12301 aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat actgatacga
    12361 cgcgcatgga caatgctgtg gtgagaatcc gccgccataa aacgcgccag gatgtgcggc
    12421 tgcccgaagt aacccagccc ccagcccatc agcgagataa tggcaacaaa gttcagtcct
    12481 ttgagcatat cgacgttttc gatgctcttt tgtttgatca cttccagcga gtcgccaaac
    12541 ccaccaacgc taatgattac gataaccggc gtcaggatca gggcaaaaat catcaggctg
    12601 gcctgtacag tgtcagtcca gctcactgca aggaagccac caataaaggt gtaaaggatc
    12661 gtcgccgcag cgcccgccca cagagccgtt tcgtagctca tgccaaaggt actttcaaac
    12721 agacgcgcgc ctgccacgat gcccgaagcg caataaatgg tgaagaacag caaaatcacc
    12781 agcgcggaga taatgcgcaa aatacggctt ttatcttcaa agcgcccggt aaaataatcc
    12841 ggcaatgtta aggcgttatt gttgtattcg gtatgcacac gcaaccgccc ggccaccagc
    12901 ttccagttaa tccacgcgcc taatgtcagg ccaatggcga tccagctttc ggaaatcccg
    12961 gaaagaaaaa ccgcgcccgg caaccccatt aacagccagc cgctcatatc cgatgcaccc
    13021 gccgataatg ccgtcacgaa tggcccaagg ctacgtccgc ccagaatata gtcgtcaaag
    13081 tttttcgttg atcgccaggc gataaaccca atcaatatca tgccaaagat atagacacaa
    13141 aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaaat tattatcggc
    13201 aatgtcgaaa cttgccgtta tatctgccac cggaacgggg tagcagagtt tatgttttac
    13261 cagggcgacc gtatcctgcc ggaagcgctg gttattcaca atcgatttaa cacaccattt
    13321 acattaaatt ttagtgctca gcgacactat ttttcatcag gttgcactct ctcacatttt
    13381 ttgcggttgc acctttcaaa aatgttaact gccgcagaga aaaagtctga gttatttttt
    13441 taatccctgt catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc
    13501 aggtcacatt taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt
    13561 aagttgcacc tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa
    13621 gctggacgac gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacgcc
    13681 acactggtta attaagcagg cgattttttc ttatcttgaa caactggaaa acagcgatac
    13741 tcttccggag ctacctgcgc tgctctctgg cgctgccaat gagagcgatg aagcaccgac
    13801 tccggcagag gaaccacacc agcctttcct cgactttgcc gagcaaattt tgccccagtc
    13861 ggtttcccgc gccgcgatca ccgctgccta tcgccgcccg gaaaccgaag cggtttctat
    13921 gttgctggaa caagctcgcc tgccgcagcc gattgctgaa caggcgcata aactggcata
    13981 tcaactggcc gataaactgc gtaatcaaaa aaatgccagt ggtcgcgcag gtatggtcca
    14041 ggggttattg caggagtttt cgctgtcatc gcaggaaggc gtggcgctga tgtgtctggc
    14101 agaagcgttg ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc gcgacaaaat
    14161 cagcaacggt aactggcagt cacacattgg tcgtagcccg tcgttgtttg ttaatgccgc
    14221 cacctggggg ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc
    14281 ccgctcgctg aaccgcatta tcggtaagag cggtgaaccg ctgatccgca aaggtgtgga
    14341 tatggcgatg cgcctgatgg gcgagcagtt cgtcactggc gaaaccatcg cggaagcgtt
    14401 agccaatgcc cgcaagctgg aagagaaagg tttccgttac tcttacgaca tgctgggcga
    14461 agccgcgctg actgccgcag atgcacaggc gtatatggtt tcctatcagc aggcgattca
    14521 cgccattggt aaagcgtcca atggtcgtgg catctatgaa ggtccgggca tttcaatcaa
    14581 gctgtcggcg ctgcatccgc gatacagccg cgcccagtat gatcgggtaa tggaagagct
    14641 ttacccgcgt ctgaaatcac tcaccctgct ggcgcgtcag tacgatattg gtattaacat
    14701 cgatgccgaa gaggccgatc gtctggagat ctccctcgat ctgctggaaa aactctgttt
    14761 cgagccggaa ctggcaggct ggaacggcat cggttttgtt attcaggctt atcaaaaacg
    14821 ctgcccactg gtgattgatt acctgattga tctcgccacc cgcagccgtc gccgtctgat
    14881 gattcgtctg gtgaaaggcg cgtactggga tagtgaaatc aaacgtgcgc agatggacgg
    14941 ccttgaaggt tatccggttt atactcgcaa ggtgtatacc gacgtttctt atcttgcctg
    15001 tgcgaaaaag ctgctggcgg tgccaaatct aatctacccg cagttcgcaa cgcataacgc
    15061 ccatacgctg gcggcaattt atcaactggc gggtcagaac tactacccgg gtcagtacga
    15121 gttccagtgc ctgcacggta tgggcgagcc actgtatgag caggtcaccg ggaaagttgc
    15181 cgacggcaaa cttaaccgtc cgtgtcgtat ttatgctcct gtcggtaccc atgaaacgct
    15241 gctggcgtat ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg
    15301 tattgctgac acctctttgc cactggatga actggttgcc gatccggtca ctgctgtaga
    15361 aaaactggcg caacaggaag ggcaaactgg attaccacat ccgaaaattc ccctgccgcg
    15421 cgatctttac ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg
    15481 tctggcatcg ctctcttctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc
    15541 aatgctggaa caatcggtcg cggctggtga gatgtcgccc gttattaacc ctgcggaacc
    15601 gaaagatatt gtgggctttg tgcgtgaagc cacgccgcgt gaagtagaac aggcgctgga
    15661 aagtgcggtt aataacgcgc caatctggtt tgccacgcct ccggctgaac gcgcagcgat
    15721 tttgcaccgc gctgccgtgc tgatggaaag ccagatgcag caactgattg gtattctggt
    15781 acgtgaggcc ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt
    15841 tctccactac tacgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt
    15901 agggcctgtg gtgtgtatca gtccgtggaa cttcccgctg gcgattttca ccgggcagat
    15961 tgctgctgca ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct
    16021 gattgccgcg caagggatcg ccattttgct ggaagcgggt gtaccgccag gcgtggtgca
    16081 attgctgcca ggtcagggtg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtgcg
    16141 cggggtgatg tttaccggtt caaccgaagt cgctacgtta ctgcaacgca atatcgccag
    16201 ccgcctcgac gctcagggtc gccctattcc gctcatcgcg gaaaccggcg gtatgaacgc
    16261 gatgattgtc gactcctccg ccctgaccga acaagtagta atagatgtgc tggcctcagc
    16321 gttcgacagt gcgggccagc gttgttcggc actgcgcgtg ctgtgcctgc aagatgagat
    16381 tgccgaccac acgttgaaaa tgctgcgcgg cgcaatggcc gaatgccgga tgggtaatcc
    16441 tggtcgcctg accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga
    16501 gcgtcatatt cagactatgc gtagtaaagg ccgtccggtg ttccaggcag tgcgggaaaa
    16561 cagtgaagat gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgattgaact
    16621 ggatgacttt gccgaattgc aaaaagaggt ctttggtccg gtgctgcatg tggtgcgtta
    16681 taaccgtaac cagctgccgg agctgatcga gcaaattaac gcttccggtt atggtctgac
    16741 gcttggcgtc catacgcgca ttgatgaaac catcgcccag gtcactggct cggcccatgt
    16801 cggtaacctg tacgttaacc gtaatatggt gggcgcagtg gtcggggtgc agccattcgg
    16861 cggcgaaggg ttgtccggca ccgggccgaa agcaggcggt ccgctctatc tctaccgtct
    16921 gctggcgaat cgcccggaaa gtgcactggc agtgacgctc gcgcgtcagg atgcagagta
    16981 tccggtcgat gcgcagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg
    17041 ggcggcaaac cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc
    17101 aggaacacaa cgattgctgc cagggccgac gggtgaacgc aacacctgga cgctgctgcc
    17161 gcgtgagcgc gtgttgtgta ttgcggatga tgaacaggat gcgctgactc agctcgccgc
    17221 cgtgctggcg gtgggcagcc aggtactgtg gccggatgac acgctgcatc gtcagttagt
    17281 gaaggcattg ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac
    17341 cgctcaaccc tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg
    17401 tgaagccgtt gccgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgaggcga
    17461 aagcaatatc cttctggaac ggctgtatat cgaacgctcg ctgagtgtga ataccgctgc
    17521 cgctggcggt aacgccagtt taatgacgat cggttaaaca ggtagccgga ggatgttaca
    17581 acctcctccg gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt
    17641 caaccgtttg attgaaaaat acctcatcgc gcaacgtcgc gcctgtcacc gcctccacct
    17701 gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat cataaaaatc aaatgctgcg
    17761 ggtcaatcgg cgcgagtttg ccgcttttta cccaaccggc aatcagcgcc gatttctcat
    17821 caattaatgc cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca
    17881 tctccataca gaacaggcgc gaagcctgcg gataatcgcg tgagacttcc agcttcagac
    17941 ggatgtactc tttgatcgcc gccagcgggg cgaaatcttc acgaaacgct tttaacggtg
    18001 ccagccagat atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa
    18061 agtaatacag cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg
    18121 tgccgtgaaa accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct
    18181 tcgcgctcac tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct
    18241 ctcccctctt gtagattggc aacaggatag caaaacccct gcccggcctc gaccttctgc
    18301 actttcatcg cgcacggatg catgttttat gtgcaactgt tttgaccgtt tagtccactt
    18361 ttttccagat attttagaca acttaacatt aacatattaa taacattcac tttttaaaaa
    18421 ctggcatccg ctttgcaaac aaaccattac gcggctgaaa aggatatgca ggatgcagcg
    18481 ccccgtctga ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc
    18541 gtacctattg gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc
    18601 tttgaactga ataaagccat tgtgcaaaaa gcggagcact accatttcga tttcgccctg
    18661 tcgatgatca aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag
    18721 tcgttcacct tgatggcggg gctggcagcc gtgacctcgc gcattcagat atacgcgaca
    18781 gctgccacct taacgttacc tccggcaatc gtcgcccgta tggccgcaac cattgactcc
    18841 atctctggcg ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgagtatgag
    18901 cagatgggta tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgaa
    18961 tatgttcagg tgctgcgcga cctgtggggc tcggggaaaa gcgattttaa aggcgatttt
    19021 ttcactatgg atgattgtcg cgtcagtccg caaccgagtg tccccatgaa agtgatctgc
    19081 gccgggcaaa gcgacgctgg catggcgttc tccgcccggt atgccgattt taacttctgt
    19141 ttcggcaaag gcgtaaatac acccacggct ttcgccccga ccgctgcgcg gatgaaacag
    19201 gccgcagagc aaaccggacg cgacgttggc tcttatgtgt tgtttatggt gattgccgat
    19261 gaaaccgacg atgccgctcg cgccaaatgg gaacactaca aagcgggcgc ggatgaagag
    19321 gcgttaagct ggctaaccga acaaagtcag aaagataccc gctccggtac tgacaccaac
    19381 gtccgtcaga tggccgatcc cacttcggcg gtaaacatca atatggggac gttagtcggt
    19441 tcttacgcca gtgtcgcgcg catgttagat gaagtcgcaa gcgtgcctgg tgccgaaggc
    19501 gtgctattga ccttcgatga ttttctgtcg ggaatcgaaa ccttcggcga gcgcattcaa
    19561 ccactgatgc agtgccgcgc ccatctccct gcgctgactc aggaggtggc atgatgacaa
    19621 tcttaaccgc tcgaccggaa gccattacct tcgatccgca gcaaagtgcg ctgattgtgg
    19681 tggatatgca aaacgcttat gccacgccag gcggctactt agatctcgcc gggtttgatg
    19741 tctcaaccac tcgcccggtc attgccaaca ttcaaaccgc cgtgaccgca gcgcgagcgg
    19801 cagggatgtt gattatctgg tttcaaaatg gctgggatga acagtatgtc gaggctggcg
    19861 gccccggctc accaaatttt cataaatcga acgccctgaa aaccatgcgt aagcagccgc
    19921 agctacaggg gaaattgctg gcgaaaggct cctgggatta tcaactggtg gatgaactgg
    19981 tgccgcagcc tggcgatatt gtgctgccta agccgcgcta cagcggtttc ttcaatacgc
    20041 cgctggacag cattttgcgc agccgcggaa tacgccatct ggttttcacc ggtatcgcta
    20101 ccaacgtctg cgtcgaatcg acgctacgcg acggcttttt tctggagtat ttcggcgtgg
    20161 tgctggaaga cgcaacacac caggcggggc cggaatttgc acagaaggcc gcgttgttca
    20221 atatcgaaac cttttttggc tgggtcagcg acgtcgaaac gttctgcgac gcgctttctc
    20281 ccacgtcctt tgctcgtatc gcttaaggag tttaacgatg ccaaaatccg taattattcc
    20341 cgctggcagc agcgcaccgc tggctccctt cgttcccggc acgctggctg atggcgtggt
    20401 gtatgtctcc ggtacgctgg cttttgatca acataataac gtgctgtttg ccgatgaccc
    20461 aaaggcgcaa acccgccacg ttctggaaac catccgcaag gtgatcgaga cggcgggtgg
    20521 cacgatggcg gatgtgacct tcaacagcat ctttattacc gactggaaaa attacgccgc
    20581 gattaacgaa atctacgccg agtttttccc gggtgacaaa ccggcgcgat tctgcattca
    20641 gtgcggactg gtaaaacctg acgcgctggt ggaaatcgcc acaattgcgc atatcgccaa
    20701 gtgaggccgc gatgaaactt tcactctcac ctccccctta tgctgatgcg cccgtagtgg
    20761 tgttgatttc gggtcttggc ggtagcggca gttactggtt accgcaactg gcggtactgg
    20821 agcaggagta tcaggtaatc tgttacgacc agcgcggcac cggcaataat cccgacacgc
    20881 tggaagaaga ttacagtatc gcccagatgg cagcggaact gcatcaggcg ctggtagccg
    20941 cagggattga gcgttacgcg gtggtcggcc atgcgctcgg tgcactggtg ggaatgcagc
    21001 tggcgctgga ttatcccgcg tcgttaactg tgctggtcag cgttaacggc tggctacgaa
    21061 taaacgccca tacgcgccgc tgttttcagg ttcgcgagca gttactacat agcggcggcg
    21121 cgcaggcatg ggtggaagct cagccattgt tcctctatcc cgccgactgg atggcggccc
    21181 gcgcacctcg cctggaagct gaagacgcgc tggcactggc gcattttcag ggcaaaaata
    21241 atttactgcg tcgacttaac gccctcaaac gcgctgactt tagtcaccat gcggatcgca
    21301 tccgctgccc ggtgcaaatc atctgcgcca gtgatgatct gctggtgcca acagcatgtt
    21361 ccagtgaact tcatgccgcc ctgcccgata gccagaaaat ggtgatgcgc tatggcggac
    21421 acgcctgcaa cgtgaccgat cccgaaacgt ttaatgctct gttactcaac gggcttgcca
    21481 gcctgttaca tcaccgtgaa gccgccctgt aaggaattgc tatgaacgaa gccattagcc
    21541 caggtgcgct tagcaccctg ttcaccgatg cccgcactca caacggctgg cgggagacac
    21601 ccgtcagcga tgagacgtta cgggagcttt atgccctgat gaaatggggg ccgacatcag
    21661 ctaactgttc tccggcacgg atcgtgttta tccgcactgc agaaggaaaa gaacgtctgc
    21721 gcccggcgct ttccagcggc aatctgcaaa aaaccctgac cgcgcccgtc accgcgatcg
    21781 tcgcctggga cagtgaattt tatgaacggt taccacagct gtttcctcac ggcgatgccc
    21841 gcagttggtt tacctccagc ccacaacttg ccgaagaaac agcgtttcgc aacagttcca
    21901 tacaggcggc ctatctgatc gtcgcctgcc gggcgctggg gctggatacc ggcccgatgt
    21961 ccggctttga ccgtcaacac gtggacgacg ccttttttac gggcagcacg ctgaagagca
    22021 atctgctgat taatatcggc tatggcgata gcagcaagct ttatgcgcgc ctgccacgtc
    22081 tgtcctttga agaagcctgc gggctgttgt aaggagcaaa ctatgaacat tgtcgatcaa
    22141 caaacttttc gcgatgcgat gtcctgcatg ggcgcggcgg tcaatattat caccacggac
    22201 ggtccagccg ggcgcgccgg gttcaccgcc agcgccgtct gcagcgtgac cgatacgccg
    22261 cccacattac tggtgtgcct gaatcgtggg gcgtccgtct ggccggtatt caatgaaaat
    22321 cgaacgctgt gtgtaaatac acttagcgcc gggcaggagc cgctttcaaa cctttttggc
    22381 ggcaaaacgc ccatggaaca ccgctttgcc gccgcccgct ggcagactgg tgtgaccgga
    22441 tgcccgcaac tggaagaggc actggtttcg tttgactgcc gtatcagcca ggtggtgagc
    22501 gtcggcaccc acgacattct gttttgtgcc atagaagcga ttcatcgtca cgccaccccc
    22561 tacgggctgg tgtggttcga tcgcagttat cacgcgttga tgcgccctgc ttgttaacct
    22621 ccttaaggag acagctgatg gcaatgttcg gttttcctca ctggcagtta aaatcgacct
    22681 ctacagagag cggcgtggtc gcgccggatg aacgattgcc gtttgctcag acggcagtaa
    22741 tgggcgttca acacgcggtg gcgatgtttg gcgcgacggt gttaatgccg attctgatgg
    22801 ggctggatcc caatctttcc attttgatgt cggggatcgg cacgttactg tttttcttta
    22861 ttaccggcgg gcgcgtcccc agttatctcg gttccagcgc ggcttttgtc ggcgtggtga
    22921 ttgccgcgac gggctttaac ggtcagggca ttaacccgaa tatcagcatc gcgctgggcg
    22981 ggattatcgc ctgtgggctg gtttataccg tcattggcct ggtggtgatg aaaatcggta
    23041 cgcgctggat tgaacggctg atgccgccgg tggtgacggg tgcggtagtg atggcgattg
    23101 gcctgaactt agcgcctatc gcagtgaaaa gtgtatcggc ttcggccttt gatagctgga
    23161 tggcggtaat gacggtgctg tgtattgggc tggtagcggt gtttacccgt ggcatgatcc
    23221 agcggttatt gattctcgta ggattaattg tcgcctgtct gctgtatggc gtgatgacca
    23281 acgttttagg gctgggaaaa gccgtcgatt ttactctcgt cagccatgct gcctggttcg
    23341 gtttgccaca tttttccact cctgctttta atggtcaggc aatgatgctg attgcgcccg
    23401 tggcggtgat tctggtggcg gaaaacttag gccatctcaa ggcggtcgcc gggatgaccg
    23461 gacgcaatat ggatccatat atggggcggg cattcgtggg cgatgggctg gcgacgatgc
    23521 tttccggctc tgtcggcggt agcggcgtga ccacctatgc tgaaaatatc ggtgtgatgg
    23581 ccgtgacaaa agtctactcg acgctggtgt ttgttgctgc tgccgtcatc gccatgctgt
    23641 tgggcttttc gccgaaattt ggcgcgctga ttcataccat tccagcaccg gtgattggtg
    23701 gtgcatcaat tgttgtgttc gggctgattg ccgtcgcagg cgcaaggatc tgggtacaaa
    23761 accgtgtcga tttaagccag aacggtaatt tgattatggt cgcagtaacc ttagtgctgg
    23821 gcgcgggtga ttttgctctc acgctaggcg gttttacgtt gggagggatc ggtacagcaa
    23881 cctttggcgc gattttactc aatgcgttgt taagcagaag gttggttgac gttccaccgc
    23941 cagaagttgt tcatcaggag ccataaacag aaaaccgccg gggtaaccgg cggtgatgac
    24001 tttttgacga gcttcagaca aaaatacatt cgtcattgat tagcccggct agttgtcgct
    24061 tttgccgtga ctgctcttgc cacctttttt acctgcttca gatgcgcgct gcgggtcatt
    24121 tttgaaatta cccccgctgt gctgtccacc ttttttacct gcttctgatg ctctttcgcg
    24181 gtcttctgca aaattgccgg aaccgcctcg atggtttgcc attactgacc tccgtctggg
    24241 tatagacatc atattgcata gaattaacgc ggcgtatggc ggctgccata ttttcgctac
    24301 gtgggactaa gcttagtgaa ctgacacggt caggcccgca tttagtaata tcctgcaaca
    24361 gcttcttagc gagaatagat atcatctcca ataattatcc ataagccgct cttatgatta
    24421 aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa aattacgcgc ttgtgaaagt
    24481 tgttataaat caaataagtg gttgtgaaat ttgcactctg aaaaggacgt cttatcttta
    24541 aaagagtggt agcgaatcgc tacggaatag agataacacg aggagtggtt agaaatggct
    24601 aaagttctgg tgctttatta ttccatgtac ggacatatcg aaacgatggc acgcgcagtc
    24661 gctgagggtg caagcaaagt ggatggcgca gaagttgtcg ttaagcgtgt accggaaacc
    24721 atgccgccgc aattatttga aaaagcaggc ggtaaaacgc aaactgcacc ggttgcaacc
    24781 ccgcaagaac tggccgatta cgacgccatt atttttggta cacctacccg ctttggcaac
    24841 atgtccggtc aaatgcgtac cttcctcgac cagacgggcg gcctgtgggc ttccggcgca
    24901 ctatacggaa aactggcgag cgtctttagt tccaccggta ctggcggcgg tcaggaacaa
    24961 accatcacat ccacctggac gacccttgcg catcacggca tggtgattgt ccccattggc
    25021 tacgcagcgc aggaattatt tgacgtttca caggttcgcg gcggtacgcc gtacggcgca
    25081 accaccatcg caggcggtga cggttcacgc cagccaagcc aggaagaact gtctattgct
    25141 cgttatcaag gggaatatgt cgcaggtctg gcagttaaac ttaacggcta atcttcaaca
    25201 ggaggatacg catgccaact caagaagcga aagctcatca cgtcggtgaa tgggcatctc
    25261 tgcgcaatac gtcgccggaa atagccgagg ccattttcga agtcgccggg tacgacgaaa
    25321 aaatggcgga aaagatttgg gaagaaggta gcgatgaagt gttagtcaaa gcctttgcta
    25381 agaccgataa agattcgctt ttttggggcg aacagaccat cgaacgtaaa aacgtttaat
    25441 acgcgactcc cccgcttctc gcgggggagt tttctgttat ttcaccgctt cattcaacac
    25501 gctatcaaac ttatccattg ggcagaaacc gttggcatct atcggacaac cgcttaattc
    25561 cagcgtcacg cgctgcgcag gtgcctgaag ggttaacgcg tcagcattac gtaactgttc
    25621 tgcactctgg tacacatact cgattttcat caggtcgcga ttggctttgc tgtcatgcca
    25681 acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg ttctggtcat gcaactgata
    25741 cggtttgaaa tccagcgccg ttaacagaga ggcaatgttg gagtcgtgcc caactaacac
    25801 cgtgattttc ggtgcactgg tgcgatcggt gaccagggct ttgtcgatat aactgaccag
    25861 cggtttcgca acattgcgcg ccacttccgg tgaggtaaac aggctgtcct ggtaaccgtt
    25921 tttaagcttc gacaacacct tccactgctg gtcagatttg atttctcccc aggccacctg
    25981 atccatcgga aaaccttcgt agtattgcaa agtaaacgca tctaccagcg agttgccgac
    26041 tttcagcggc ccggaaacac ctggttcttg ttgatacttc gcgctaaagg tatttttgcc
    26101 atccaccagc gaacattgtt gtttctcttt acaggcaggg gaatctttat agttaacgat
    26161 tttttccagt agctggtagc tgtcggtaag ctggagtttg ctgagctctt tctccattgc
    26221 cgccaccgcc tgttcactga atgcggcgga atcatcggtg atcactgggt taaaggttgg
    26281 gtccatagtg cccatttttt cctggtgatg cacaggaata tcacacccgg ggaatgcgcc
    26341 ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga ctattggcat aggcatatac
    26401 ggtgtccggc ggcgggcatt cccccgattt taccatcccc tgctctgcca gccattcacg
    26461 catgtaatgg cccatataca cttcgagcac gccacctttg gtggtgagtt gcccaccggg
    26521 gacgtcccat tctggccatt tattcggcgt cgactgctcc agcacactgc cattgttcgc
    26581 cagcggcgca cgtaagttat ggcggctcat catgagcact tgctgtagct gatagccttc
    26641 cggtacggtt tgtgcctgag catttgaagc gagtaaaact atccctgcca cagttgcggc
    26701 gattagcgtt ttgttcattc ctggcacctc ttttgttatc aatcactaaa gagtgtgaca
    26761 gaaatatgac ttttttccgg gaacaggttc gcaaaatggc ttgttgagcg gctgtttttg
    26821 tatcttcatc tttaaaaaca ggcaagttat gacgcaaact atgcctgatt aacccctgcc
    26881 tcatgcacgc cgctgatgat tacacctgtg gctaaacaga atatcagcgg cgttaccaat
    26941 acagaggcaa tatttaccaa taaaaaagtg tgagactaat aacaatggtt tttagcacat
    27001 gcatttgtca ttaaattgca taacgagtta aaaaatcaat ttgcgcattc ctttccttat
    27061 tgtttaactt gtaagcctga gcaaaaagtt caggagggag gtaaacgtca taatgattgc
    27121 cggtatttat taaataacca tattcacgat cttcatctgt tgaaaaggat tcatttccca
    27181 aggagattga tgccacatta ctattaacaa taaataatga gcaaatgcta tctgaaagaa
    27241 cagcaagaaa attcttcacc gctttaatcc tatccgacgt gtcggtatct aaagcaagca
    27301 aattcttgtt atcattaaat ataaactcgg gtaaatgcag gatacatttt tcgttattca
    27361 agcaactatc aatgaattct tttttaaaaa acagaaagct ccatgacata agactaccca
    27421 caccctgaat tttgttgtca gattgcagat caacgccaaa taatgtctta acatcttccc
    27481 tgagatcaca aaatgcagca tcaacaagag cctcgattgt tggtttatca acaattttaa
    27541 taaactcttc gtatacttcc ttttgcgt
//
LOCUS       DARNVM010000051        27007 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.051, whole genome shotgun sequence.
ACCESSION   DARNVM010000051 DARNVM010000000
VERSION     DARNVM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.051"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..88
                     /locus_tag="SI621_001952"
     CDS             <1..88
                     /locus_tag="SI621_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acrEF/envCD operon transcriptional regulator"
                     /protein_id="HEI4064679.1"
                     /translation="LVDNVLRMFMPDENITKLIHQTNELSVI"
     gene            complement(91..270)
                     /locus_tag="SI621_001953"
     CDS             complement(91..270)
                     /locus_tag="SI621_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HEI4064680.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(354..1238)
                     /gene="yhdJ"
                     /locus_tag="SI621_001954"
     CDS             complement(354..1238)
                     /gene="yhdJ"
                     /locus_tag="SI621_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HEI4064681.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1324..1620)
                     /gene="fis"
                     /locus_tag="SI621_001955"
     CDS             complement(1324..1620)
                     /gene="fis"
                     /locus_tag="SI621_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEI4064682.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1646..2611)
                     /gene="dusB"
                     /locus_tag="SI621_001956"
     CDS             complement(1646..2611)
                     /gene="dusB"
                     /locus_tag="SI621_001956"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEI4064683.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2940..3821)
                     /gene="prmA"
                     /locus_tag="SI621_001957"
     CDS             complement(2940..3821)
                     /gene="prmA"
                     /locus_tag="SI621_001957"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEI4064684.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3833..5284)
                     /gene="panF"
                     /locus_tag="SI621_001958"
     CDS             complement(3833..5284)
                     /gene="panF"
                     /locus_tag="SI621_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HEI4064685.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5274..5516)
                     /locus_tag="SI621_001959"
     CDS             complement(5274..5516)
                     /locus_tag="SI621_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HEI4064686.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5625..6974)
                     /gene="accC"
                     /locus_tag="SI621_001960"
     CDS             complement(5625..6974)
                     /gene="accC"
                     /locus_tag="SI621_001960"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEI4064687.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6985..7455)
                     /gene="accB"
                     /locus_tag="SI621_001961"
                     /gene_synonym="fabE"
     CDS             complement(6985..7455)
                     /gene="accB"
                     /locus_tag="SI621_001961"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEI4064688.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8432..9406)
                     /locus_tag="SI621_001962"
     CDS             complement(8432..9406)
                     /locus_tag="SI621_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEI4064689.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILSRDEFAESRPLEKQLWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAGAIINNQIQGRTLVKVN"
     gene            9558..11498
                     /gene="csrD"
                     /locus_tag="SI621_001963"
     CDS             9558..11498
                     /gene="csrD"
                     /locus_tag="SI621_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HEI4064690.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCERS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11803..12846
                     /gene="mreB"
                     /locus_tag="SI621_001964"
     CDS             11803..12846
                     /gene="mreB"
                     /locus_tag="SI621_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEI4064691.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12912..14015
                     /gene="mreC"
                     /locus_tag="SI621_001965"
     CDS             12912..14015
                     /gene="mreC"
                     /locus_tag="SI621_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEI4064692.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQLATGNAATA
                     PTAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14015..14503
                     /gene="mreD"
                     /locus_tag="SI621_001966"
     CDS             14015..14503
                     /gene="mreD"
                     /locus_tag="SI621_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEI4064693.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14512..15105
                     /gene="yhdE"
                     /locus_tag="SI621_001967"
     CDS             14512..15105
                     /gene="yhdE"
                     /locus_tag="SI621_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HEI4064694.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAVQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15095..16564
                     /gene="rng"
                     /locus_tag="SI621_001968"
     CDS             15095..16564
                     /gene="rng"
                     /locus_tag="SI621_001968"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEI4064695.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16632..20432
                     /gene="yhdP"
                     /locus_tag="SI621_001969"
     CDS             16632..20432
                     /gene="yhdP"
                     /locus_tag="SI621_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HEI4064696.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWNQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALNGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGIELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20588..22033
                     /gene="tldD"
                     /locus_tag="SI621_001970"
     CDS             20588..22033
                     /gene="tldD"
                     /locus_tag="SI621_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEI4064697.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22161..23090)
                     /gene="aaeR"
                     /locus_tag="SI621_001971"
     CDS             complement(22161..23090)
                     /gene="aaeR"
                     /locus_tag="SI621_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HEI4064698.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23273..23476
                     /locus_tag="SI621_001972"
     CDS             23273..23476
                     /locus_tag="SI621_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HEI4064699.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23484..24416
                     /gene="aaeA"
                     /locus_tag="SI621_001973"
     CDS             23484..24416
                     /gene="aaeA"
                     /locus_tag="SI621_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HEI4064700.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24422..26389
                     /gene="aaeB"
                     /locus_tag="SI621_001974"
     CDS             24422..26389
                     /gene="aaeB"
                     /locus_tag="SI621_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HEI4064701.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26481..26753
                     /locus_tag="SI621_001975"
     CDS             26481..26753
                     /locus_tag="SI621_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEI4064702.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26809..>27007)
                     /locus_tag="SI621_001976"
     CDS             complement(26809..>27007)
                     /locus_tag="SI621_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4064703.1"
                     /translation="ADSIDAAQAQNREAIGTVSVSGVASSPMDMREMLNKKAEEKGAT
                     AYQITEARSGDTWHATAELYK"
BASE COUNT         6443 a         6805 c         7217 g         6542 t
ORIGIN      
        1 actggtcgat aacgtattaa gaatgttcat gccagatgaa aacataacga aattaattca
       61 tcaaacgaat gaattaagtg tcatataaat tcacattctg tcctgaaaat tcagttcgtc
      121 acagttaacc agttttagtg gattaactga gttcatattg cggcatttgt ctgtttctgt
      181 tgcgagcagt tcaatccact gcatcagtaa agtatcaaac agaaaaacga agacagcgaa
      241 aacgacgagc caccaatact tgcgaatcat tacctgagcc tgtgcatgtt taaccaagtc
      301 tggcaggaaa tatacacgaa atgaataatc tgaaaaccag gcttacactt cctttacttt
      361 gcaatgagat cggggtcaac ttcgcttaac ctgcttcgtt ttgacaggtt gccggtcttt
      421 ctttttttca ctttcgccag ttcttccgca gagtaatgcg acgcgacatc cagccgtcga
      481 agccccattt tgatgtactc gctgttgatc tcaataccaa tgaattttcg cccactggcg
      541 acggctacgg caccggtagt aaagctacca gcaaacgggt cgagaacgat atcgcctggg
      601 ttggaagagg cgagaataat gcgtttcagt aaggcttccg gtttttgcgt cgggtggttt
      661 tcatattcat ccatcaaata acgcacgcgc ggaaaatccc agacgttacc cggtactttt
      721 tgatgattgt aaggctgtgg agggtttttg cgataatcga tcaacgcgcg ctgcgatccg
      781 gttttggctt ctaccagaat agcatcaccg ttgaatgtgt agttctttgc gtctttcacc
      841 atcatcagga tgggttcgta catggagccg tagtgttttt tcgcctgcac tccagaactg
      901 tcatatgacc agacgatgcg acttttgatg gtaaaaagct tgcggcactg gagatcgata
      961 aagggcatgt tttccgtgct attcatgatg tacatgctgc cctgcttttt cagaacgcga
     1021 tggcactctg caatcacttc aaacagccag tcgataaaca gatcttcttt ccaggcttcg
     1081 atcagaccat caaaattttt accgatgtta tacggtgggt cggcaaagat cagatcgaca
     1141 ctttcggcgg ggagcttttt aagttcggtt aatgcatcac cctggatgat ggtctttgct
     1201 tcattaccaa atcgggtcgg ttcacatcct gttctcatgg tcactccctt tgtgacacct
     1261 ataaaaaagg cgcttcccca tgccgagtag cgccttttta atcaagcatt tagctaacct
     1321 gaattagttc atgccgtatt ttttcaattt tttacgcagc gtaccacggt tgatgcccat
     1381 catcagcgca gcacgggtct ggttaccacg ggtgtattgc atcaccatgt ccaacagggg
     1441 ctgttctact tcagccagta ccagctcata gaggtcattc acatcctgac cgttcagttg
     1501 agcaaaatag ttcttcagtg cctgtttaac cgagtcacgc aggggttttt gggttacctg
     1561 atcctgagag ttaacggtag aaacggtcag tacgtcagaa tttacgcgtt gttcgaacat
     1621 agttctgtca gctctttatt tctgtttacg caaaattttc gaagtatgcc tccaacgcct
     1681 ccagctgttc gctggcatcc tcaatggcgt tgaatgtgcg ccgaaactgg tcatttggag
     1741 cgtgttcctg gagataccag gaaacgtgtt tacgtgcaat tcggtaccct tttgccggac
     1801 cataaaagtc atgcagttcc cgaacgtgcg cgcaaagcaa gcgcttaacc tctgccaaag
     1861 gcagcggggg cagcaactcc ccagtgtcca gataatgctg gatttcccga aagatccagg
     1921 gtcttccctg agctgcgcgg cctatcatca gggcatccgc ccctgtatag tcgagcacag
     1981 ctctggcttt aagcgggtca gtaatgtcgc cattcgcgat aaccggaatg gaaactttct
     2041 gcttaactgc ccgaatactg tcgtactcag cttctccatt gaacaaacag gcgcgtgtac
     2101 ggccatgaat ggtcagagcc tgaatgccac agtcttcagc cagttgggca atctcttcgc
     2161 agttacggtg ttccggtgcc cagccagtgc gaattttcag ggtaacagga acgtccactg
     2221 cattgacgac ctcggtaagg atcgatttaa cgacatccgg gtactgcaag agggctgaac
     2281 ctgcgagctt gcgattcact tttttagccg ggcaacccat attgatatca ataatctggg
     2341 caccgctttc cacgttaata cgtgctgcat ctgccatttc tttcggatcg ctaccagcaa
     2401 tttgcacggt gcgaataccg ggttcatcaa tgtgcaccat ccgtaaacga gatttgtcgc
     2461 tttcccaaac ctgagggtta gaagacatca tctcggatac tgtcaatccg gctcccatct
     2521 cgtagcacaa cgtccgaaaa ggtctgtctg taatgccagc catgggcgct gcgatcaggc
     2581 gatttctgag ctgatattgt ccgatgcgca tgagttaaga aatgaccata ctgtgactgc
     2641 aaggcggcgt atattacgca ttttttgcac gagatgaaag gccaaacttt gaccaatcct
     2701 ctgagatgga tcaaagaatt gcatttaaaa tgagcgtagc gcgataatta ctcataaaaa
     2761 tcatcatatt agaaaatagt gactaaaatt tacactcaaa gaaatttgag taagttctca
     2821 ctttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc ggcaaattca
     2881 cgtgcctttt gtgaccttgc tcgcacttcg ccctgcgtca ccctacggcg atgcgaaggt
     2941 taattcttac gaccggtaat gcggcaccac tcttcttttt ccacgaccgg gtccagagcg
     3001 aagctgtcgg cataagcttc gcaaacgctc tctgcctggc ttgccagaat accggaaagg
     3061 cccagcaaac cgcctgaaac tggcaggacg ctgattaacg gtgccagttc acgtaatggg
     3121 cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg atctttcggt
     3181 aagtagagtt ccagacggtc agagacgcca ttacgttccg cgttgtcgcg gctggcctga
     3241 atcgcctgcg gatcaatatc aataccaatg gcttttgctg cacccagttt cagcgccgcg
     3301 atagccagaa tgccggaacc gcagccaaag tcgatgactg ttttaccggt taaatcgagg
     3361 ctgtcgagcc attgcaggca cagagaggta gttggatggg tacccgtacc aaacgccagc
     3421 cctgggtcta acatcacgtt gacggcgttt tcgtccggca catcacgcca gcttgggcag
     3481 atccacagtc gttcaccaaa gcgcatcggg tggaaattat ccatccattc gcgctcccag
     3541 tctttatctt ccagttgttc gattttatgc gcgaagcccg cgccgagcag cggatggttt
     3601 tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc aatcacatcg
     3661 gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa atacaggcgt atcgtgggta
     3721 tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag atcttccgcg
     3781 ttcgcgccgg tagtgttcag tttcagttgg atccaaggca tggcaaaact ctttatttat
     3841 cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg accaggaaag
     3901 ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac tgaatattca
     3961 gcgtcgcgag tacggcatac agtacgccgc caacaatcat cgcacttagc gcgcctttgg
     4021 cgttggcgcg ttcccagtaa agacccagta ccagcggcca caggaaaacg gcttccagcc
     4081 caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag gcggcaagca
     4141 gcagcaacgc accgagaact aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt
     4201 tttgcatttg atccggacgg atattcaaat agagatcttt aatgatcgta gcggaacttt
     4261 gcagcaactg ggcattgatc gtcgacatga tcgcagccat aggtgcagcc aggaagatcc
     4321 cggcagcaaa cggtggcaac acttttacca ttaacgtcgg gatcaccagg tccggtacgg
     4381 tgagatcggg gatcaccgcc cgacctaacg ctccggccag gtgcataccg aacatcagga
     4441 ttgcgaccac aatcgtaccg atgatgatcc cccgatgtac ggctttgctg tctttataag
     4501 agatacagcg caccgcagta tgtggcaggc caatcacgcc aaaacacacc agcacccaga
     4561 acgacgtcat aaacgcaggc gacagaatat cgtcagcgcc ttgtggcgta accagttgcg
     4621 gatcgatggt ttgcagcgtc tctaccgcat tgcttaagcc gccagcagca tggaccacgc
     4681 caataagcag cacaatggtg ccaatcagca tcaccagccc ttgcatggtg tcgttcagca
     4741 cgctggcgcg aaagccgccg aaggcggtgt agagcgcaat gctgatgcca aaaatcagca
     4801 aacctgtttc ataaggaata cccgccgcgg tttccagcag acgcgcaccg ccgataaact
     4861 gcacggtcat cgcaccaacg aacgcaacca gcaaacttaa actcgccagc cacaccagca
     4921 gacgactctg gtagcgggca aatagcatat cgttcagcgt caccgcattg tagcggcgcg
     4981 caagaatcgc gaacttcttg ccgagaatac cgagcgaaag ccagaccgca ggaagctgaa
     5041 tcatcgccag caatacccaa cccaggccgt atttataagc agcgccaggc ccgccgataa
     5101 acgaactggc actgatatag gtcgcggtga gcgtcatcgc cagcacaata ccgcccatag
     5161 agcggctgcc gaggaaatac tcattaagga aggtgcctgt gctccgttta cgcatcgcat
     5221 aaacagagat accgaacacc accaccagat aggcgaccag cggtagaatc acttcaagct
     5281 gcatcgtcat cctccagtgg gatatcgcga tagataaatt tcaccatcgc ccagcacagt
     5341 ccaataaaca gcagcggcgt caggatgcag gccatctcaa accagcgcgg aaagccggta
     5401 aaaccggggg caacgccagg taagtaagcg gctactaacc ataccgccag atacaaaagg
     5461 gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt gtccattttt
     5521 tgtccctgat gggtgaagaa agcggggatt gtaccttatg ggggttgtcg atccccagta
     5581 ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt ttcctgaaga
     5641 ccgagttttt tctccagata gtggatatta gtgccaccat gctggaagtt ctcgtcattc
     5701 atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc
     5761 gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg
     5821 atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgc
     5881 acgccaaaac cgcctggtgc gtggaaacgg gtgattttgc ccggacttgg caggaaggtg
     5941 ttcggatctt cggcgttgat acggcattcc accgcatggc cgcgaacgtg aacttcttct
     6001 tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat caggtcaacg
     6061 ccggtgatca tttcggtaac cggatgttct acctgaatac gggtgttcat ttcgatgaaa
     6121 tagaactcgc cgttttcgaa caggaactcg aaagtacctg cgccgcgata gccgatatca
     6181 acacacgctt tagcacaacg ttcgccgatg tagcgacgca gttccggggt aatgcccggt
     6241 gccggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttctgcc
     6301 agatagatag cgttgccctg accgtcagcc agtacctgaa tctcgacgtg gcgaggattt
     6361 tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg
     6421 gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgacca
     6481 ccgccgccgc cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatagcgcgg
     6541 tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg aacgcccgct
     6601 tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc
     6661 gggccgatga agataaagcc ggagcgctca acctgctcgg caaagttggc gttctcggag
     6721 aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt
     6781 gccgggatgt tcagataact ttttactgac ggagcagggc cgatacagac cgtttcatct
     6841 gccagtaata cgtgttttaa atcgcgatcc gcgctggagt gcacagcgac agtcttgatg
     6901 cccaattctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca
     6961 attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac
     7021 cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg
     7081 gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc
     7141 cacttcgatg aacgctttcg cgtccgggct tggggtgcgg tagaaagtac caaccatcgg
     7201 ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt
     7261 cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg
     7321 ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc
     7381 ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat
     7441 cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt
     7501 tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca
     7561 ggccgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac ggacattgtg
     7621 cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca
     7681 ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc
     7741 gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacag ctaacatgta
     7801 aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat
     7861 catcgacgga acttcttata acgtaaggct aaaagctgta aagccagccc tgcgaagatg
     7921 agggctgcaa tgagataatc ttcacagacc acaaataatg tagggatgcc aggatcgcga
     7981 caagatagac gacattacgc agcggttgct ggggattgcc caattttcac tgcatcgtct
     8041 ggataaacgt gaatgctaaa gcacgcaaaa tcaaccgcct gacaatgctc agcatcaaat
     8101 acgttcgagt ggttaatttg taggcaaata aaatcagatt attcacccca tggtaccaga
     8161 aatcagtaac aggttaagcg tagcgtcgcc caggcaaagc accataaacc taacaggcga
     8221 cggaggcgta tcaataatgg ctgtttagtg gagtaacagt gcgcatcaat ttatggtcag
     8281 tcgaaatcat gactttccag tttggatgat gttgtacaac ttcatgacca agatgttcga
     8341 cgaaagctgt ccggctaacc gcttttcccg ttaaccgatt gaataatgat ttcaaaatga
     8401 gttccctctc ttattattcc ctgctaaatg gttagttaac cttcaccagc gtgcgaccct
     8461 ggatctggtt attaatgatg gccccggcaa atttcggtgc ctctgccaga gatatctctt
     8521 ttgccgcctg ggtatagaat gattccggta aatcggcgac cagtcgctgc caggcttgtg
     8581 cgcggcgttc tggtggagtc atcactgaat ccaccccctg taaacggaca ttacgcagaa
     8641 taaatggcat gaccgtggtt ggcagagtaa aaccacccgc cagaccacag gccgccacgc
     8701 agccgccgta attcatttgc gctagcactt tcgccagcac tttgtcgcca acggtgtcaa
     8761 ttgccccagc ccagagttgt ttttccagag gacgggattc ggcaaactca tcgcgagaga
     8821 gaatacggct ggctcctaaa cttttcagat attcatgggt actttcgcga ccagaaacgg
     8881 caacgacctg ataacccaac ttatgcagca gcgccacggc ggtactgccg acgccaccac
     8941 tggcacccgt tacgacaatc tcgccgtcct gcgggcgaac accggcatct tccagcgcca
     9001 tcacacacag catggcggta aaaccggcag taccgataat cattgcttta cgcgcgtcca
     9061 gcccttgcgg catggcaacc agccagtcac ctttcactcg cgcctgctcc gccagcccac
     9121 cccagtggtt ttcaccaacg ccccagccag tgagtaatac ctcctgaccg gcatgaaaac
     9181 gcggatcttc gctggtgcgt acagttccgg caaaatcgat cccaggaatc atcggaaaat
     9241 tacggatgat ttttccctta ccggtaattg ccagcgcatc tttgtagttc aggctcgacc
     9301 agtgaacatc aaccgtgaca tcgccctccg gcaggcgact ttcgtccaga gtctgtaccg
     9361 atgcgagagt tttgccgtcc tgctgttcta aaagtaacgc ctgcataagt ggtcctcatg
     9421 tgcatgatgg attggaaaat aatttctgaa gactatactc gctaatggaa aagcaaagcc
     9481 gatgaagcgc aataaattgc gagataaatc tgatttgcta gtatgcccgc ttcctcacta
     9541 tcggagttaa cacaaggatg agattaacga cgaaattttc ggcctttgtt acgctgctca
     9601 ccgggttaac aatttttgtg actttgctgg gctgttcgct aagtttctac aatgccattc
     9661 agtataagtt tagtcatcgc gttcaggcgg tggcgacggc aattgatacc caccttgtgt
     9721 cgaatgactt cagcgcatta aggccacaaa ttaccgaatt aatgatgtcg gcagatatcg
     9781 ttcgtgtaga cctgctgcat ggtgataaac aggtatatac cctggccaga aatggaagtt
     9841 atcgtccggt tggcaccagc gatctgtttc gcgaactgag cgttccgttg ataaagcatc
     9901 cggggatgtc gctgcgtctg gtttatcagg atccgatggg caactatttc cattcgttga
     9961 tgaccaccgc gccgctcacg ggggcgattg gctttatcat tcttatgctc tttctggcgg
    10021 tacgctggtt acaacggcaa cttgccgggc aagaattgct ggaaacccgg gctactcgta
    10081 tcttaaacgg tgagcgtggc tctaatgttt tgggaaccat ctatgaatgg ccgcccagaa
    10141 ccagcagtgc gctggatacg ctgcttcgtg aaattcagaa cgcacgcgaa caacacagcc
    10201 gtcttgatac gctgatccgc tcttatgccg cccaggacat gaaaaccggc ctcaataacc
    10261 gactcttctt cgataatcag ttagcaacgt tactggaaga tcaggagaaa gtaggtaccc
    10321 acgggatcgt gatgatgatt cgtctgccgg atttcaatat gttgagtgat acctgggggc
    10381 acagccaggt tgaagaacag ttcttctctc tgacgaatct gctatcgaca tttatgatgc
    10441 gctaccctgg cgcactgctg gcgcgttacc accgcagtga ttttgctgcg ctgttaccgc
    10501 accggacgtt aaaagaggca gagagcatcg ccggtcagtt aatcaaagcc gtcgatacct
    10561 tgccgaacaa taaaatgctc gatcgcgacg atatgatcca cattggtatc tgcgcctggc
    10621 gtagtggtca ggataccgag caggtaatgg aacatgcaga gtctgccacg cgtaatgcgg
    10681 gattgcaggg cggcaatagc tgggctattt acgatgactc gttgcctgaa aaaggacgcg
    10741 gtaatgttcg ctggcgtacg cttatcgagc aaatgctgag tcgcggcggc ccgcgccttt
    10801 atcaaaaacc ggcggttact cgcgaaggtc aggttcatca tcgcgaactc atgtgccgca
    10861 tcttcgatgg taatgaagag gttagctcgg cggagtatat gccgatggtc ttgcagtttg
    10921 gcttatcgga agagtatgac cgcctgcaaa tcagccgtct tattccacta ttgcgttact
    10981 ggcctgagga aaatctggcg attcaggtta ctgttgagtc gctgattcgc ccgcgttttc
    11041 agcgttggct gcgagatacg ttaatgcaat gtgaaagatc gcaacgaaaa cgcataatta
    11101 ttgaacttgc agaggccgat gtaggtcaac atatcagtcg cttacaacct gttattcgtt
    11161 tagtgaatgc tttaggggta cgggtagccg tcaaccaggc tggtttgacg ctggtaagca
    11221 ccagttggat caaagaactt aatgttgagt tactcaagct ccatccgggg ctggtcagaa
    11281 acattgagaa gcgaacggag aaccagctgc tggttcaaag cctggtggaa gcctgctccg
    11341 ggaccagcac ccaggtttac gccaccggcg tgcgttcgcg aagcgagtgg cagaccctga
    11401 ttcagcgcgg tgttacaggt gggcaagggg attttttcgc gtcctcacag ccacttgata
    11461 ctaacgtgaa aaaatattca caaagatact cggtttaacc tgccgtttaa tccgttttca
    11521 cgtagaataa cgcgcgctgc gtctcatggg agtgtgcttg tctgctcgcc agattgttgc
    11581 agcacatatg cagatgaatg accttacgcg gttgcaaaca ggcgaggaat gctgctgatg
    11641 cattaagcct ttctggactc aggcagagat ttgtaacaaa ggaaacgaac tgcactaatt
    11701 ttcaccgtag cagatgattt ttgcgccttg tcgctgctgc gtgtggttgg taaagtaagc
    11761 ggattttctt ttccgcccca gctttcagga ttatccctta gtatgttgaa aaaatttcgt
    11821 ggcatgtttt ccaatgactt gtccattgac ctgggtactg cgaataccct catttatgta
    11881 aaaggacaag gcatcgtatt gaatgagcct tccgtggtgg ccattcgtca ggatcgtgcc
    11941 ggttcaccga aaagcgtagc tgcagtaggt catgacgcga agcagatgct gggccgtacg
    12001 ccgggcaata ttgctgccat tcgcccaatg aaagacggcg ttatcgccga cttcttcgtg
    12061 actgaaaaaa tgctccagca cttcatcaaa caagtgcaca gcaacagctt tatgcgtcca
    12121 agcccgcgcg ttctggtttg tgtgccggtt ggcgcgaccc aggttgaacg ccgcgcaatt
    12181 cgtgaatccg cgcagggcgc tggtgcccgt gaagtcttcc tgattgaaga acctatggct
    12241 gccgcaattg gtgctggcct gccggtttct gaagcgaccg gttctatggt ggttgatatc
    12301 ggtggtggta ccactgaagt tgctgttatc tccttgaacg gcgtggttta ctcctcttcc
    12361 gtgcgcattg gtggtgaccg ttttgatgaa gctatcatca actatgtgcg tcgtaattac
    12421 ggttctctga tcggtgaagc caccgcagaa cgtatcaagc atgaaatcgg ttcggcttac
    12481 ccgggcgatg aagtccgtga aatcgaagtt cgtggccgta acctggcaga aggtgttcca
    12541 cgcggtttta ccctgaactc caacgaaatc ctcgaagcac tgcaggaacc gctgaccggt
    12601 attgtgagcg cagtaatggt tgcactggaa cagtgtccgc cggaactggc ttccgacatc
    12661 tccgagcgcg gcatggtgct caccggtggt ggcgcactgc tgcgtaacct tgaccgtttg
    12721 ttaatggaag aaaccggcat tccagtcgtt gttgctgaag acccgctgac ctgtgtggcg
    12781 cgcggtggcg gcaaagcgct ggaaatgatc gacatgcacg gcggcgacct gttcagcgaa
    12841 gagtaatcgg atgcaggcag gggaaatgtc tgtttaccct gcctggtctg atacgagaat
    12901 acgcataact tatgaagcca atttttagcc gtggcccgtc gctacagatt cgccttattc
    12961 tggcggtgct ggtggcgctc ggcattatta ttgccgacag ccgcctgggg acgttcagtc
    13021 aaatccgtac ttatatggat accgccgtca gtcctttcta ctttgtttcc aatgctcctc
    13081 gtgaattgct ggatggcgta tcgcagacgc tggcctcgcg tgaccaacta gaacttgaaa
    13141 accgggcgtt acgtcaggaa ctgttgctga aaaacagtga actgctgatg cttggacaat
    13201 acaaacagga gaacgcgcgt ctgcgcgagc tgctgggttc cccgctgcgt caggatgagc
    13261 agaaaatggt gactcaggtt atctccacgg ttaacgatcc ttatagcgat caagttgtta
    13321 tcgataaagg tagcgttaat ggcgtttatg aaggccagcc ggtcatcagc gacaaaggtg
    13381 ttgttggtca ggtggtggcc gtcgctaaac tgaccagtcg cgtgctgctg atttgtgatg
    13441 cgacccacgc gctgccaatc caggtgctgc gcaacgatat ccgcgtgatt gcagccggta
    13501 acggttgtac ggatgatttg cagcttgagc atctgccagc gaatacggat attcgtgttg
    13561 gtgatgtgct ggtgacttcc ggtctgggcg gtcgtttccc ggaaggctat ccggtcgcgg
    13621 ttgtctcttc cgtaaaactc gatacccagc gcgcttatac tgtgattcag gcgcgtccga
    13681 ctgcagggct gcaacgtttg cgttatctgc tgctgctgtg gggcgcggat cgtaacggcg
    13741 ctaacccgat gacgccggaa gaggtgcatc gtgttgctaa tgaacgtctg atgcagatga
    13801 tgccgcaggt attgccttcg ccagacgcga tggggccaaa gttacctgaa ccggcaacgg
    13861 ggatcgctca gccgactccg cagcaactgg cgacaggaaa tgcagctact gcgcctactg
    13921 cgccgacaca gcctgctgct aatcgctctc cacaaagggc tacgccgccg caaagtggtg
    13981 ctcaaccgcc tgcgcgtgcg ccgggagggc aatagtggcg agctatcgta gccagggacg
    14041 ctgggtaatc tggctctctt tcctcattgc gctgttgctg caaatcatgc cctggccgga
    14101 taacctgatt gttttccggc caaactgggt gttactcatc ttgttgtatt ggatcctggc
    14161 cttgcctcat cgcgtaaatg tgggcacagg ttttgtgatg ggtgccatac tggatctgat
    14221 cagcggctcg acgcttggcg tacgcgtatt ggcgatgagc atcattgctt acctggtggc
    14281 gctgaaatat cagcttttcc gcaacctcgc attatggcag caggcgctgg tcgtcatgtt
    14341 gctttcgctg gtggtggata ttattgtttt ctgggcagag tttttagtga ttaacgtctc
    14401 tttcagaccg gaagtgttct ggagtagtgt agtcaatgga gtgctctggc cgtggatttt
    14461 cttgctgatg cgtaaagtcc gtcagcagtt tgcagtgcaa taaaggtttc tatgacttct
    14521 ctgtatttag cttccggttc tccgcgtcgt caggagttac ttgcgcaact tggcgtgacc
    14581 tttgaacgta ttgttacggg cattgaggag cagcgtcagc cgcaggagag cgcgcagcag
    14641 tatgttgtgc gtctggcgcg cgagaaagca caggcgggtg tcgcgcaaac ggcgcaggat
    14701 ctcccggtgc tgggtgcgga cactatcgtt atcctgaacg gagaagtgct ggagaaaccg
    14761 cgcgacgcag agcatgcggt gcagatgttg cgcaaattat cgggtcagac ccatcaggta
    14821 atgacggcag tggcgttggc cgacagccag cacattctcg attgcctggt ggtcaccgat
    14881 gtgactttca gaacgttaac agacgaagac atcgcgggct atgtcgccag cggtgaaccg
    14941 ttagataaag caggtgcata cggtattcag gggctgggtg gctgttttgt caggaagata
    15001 aatggcagct atcacgccgt agtcggctta ccgctggttg aaacgtatga attattaagt
    15061 aattttaacg cactgcgtga gaaaagggat aaacatgacg gctgaattgt tagtaaacgt
    15121 aacgccttcg gaaacgcgag tggcgtatat tgatggcggt attctgcagg aaattcatat
    15181 tgaacgtgag gcgcgacgcg gaatagtagg caatatctac aagggtcgtg taagtcgtgt
    15241 acttccgggt atgcaggcgg cttttgtaga tattgggctg gataaagccg cgtttcttca
    15301 tgcatctgac atcatgccgc acaccgaatg tgtggcgggt gaagaacaaa agcaattcac
    15361 ggtgcgcgac atctcggaac tggttcgtca ggggcaagat ctgatggtgc aggtggtgaa
    15421 agatccgctt ggcactaagg gggcgcgcct gaccacggat atcacgctgc cttctcgcta
    15481 tctggtattt atgccagggg cttctcacgt tggggtttcc caacgaattg aaagcgaatc
    15541 agaacgtgaa cgcctgaaaa aagtggtcgc agagtattgc gacgagcagg gcgggtttat
    15601 catccgtacc gcagctgaag gggttggcga ggctgaactg gcctccgatg ccgcttatct
    15661 gaaacgcgtc tggaccaaag tgatggagcg taaaaagcgc ccgcagactc gttatcagct
    15721 gtacggcgaa ctggcgctgg cgcagcgtgt tctgcgtgat ttcgccgacg ccgaactgga
    15781 tcgcattcgc gttgactcac gcctgactta cgaagcatta cttgagttca cctcggagta
    15841 cattcccgag atgaccagca agctggagca ttacaccgga cgccagccga ttttcgatct
    15901 ctttgatgtc gaaaacgaaa tccagcgagc gctggaacgc aaagtagaac tgaaatccgg
    15961 tggttatctg attatcgacc agaccgaagc gatgaccacc gtggacatca ataccggagc
    16021 gtttgtcggt catcgcaatc tggacgacac cattttcaat accaatattg aagcgacgca
    16081 ggctatcgct cgccagttac ggttgcgcaa tctgggcggg attatcatta ttgatttcat
    16141 cgacatgaat aatgaagatc accgtcgccg cgtgttgcat tcgctggagc aggcgttgag
    16201 caaagaccgg gtgaaaacca gcgttaatgg tttttcggcg ctggggctgg tggagatgac
    16261 gcgtaaacgc acccgcgaaa gcattgagca cgtactatgt aacgaatgcc caacctgcca
    16321 cggtcgcgga acggtgaaaa ccgtggaaac ggtatgctat gaaatcatgc gcgagattgt
    16381 tcgtgtacac catgcttacg attccgaccg tttcctggtc tatgcttccc cggcagtagc
    16441 tgaagccttg aaaggcgaag agtcacactc gctggcggaa gtggaaattt tcgttggtaa
    16501 acaggttaaa gtacaaattg aaccgctcta taaccaggag cagtttgacg tcgtaatgat
    16561 gtaaacagat gctgggccgc catccggcaa agggtttttg agtcacattt ttagcagaca
    16621 aggagtgacg ggtgaggcga ttgccgggga tattactgct tactggagcc gcgctcgttg
    16681 tgatcgctgc cctgctggtt agcggcctgc gtattgcttt accgcatctt gatgcctggc
    16741 gtccggaaat cctcaacaaa atagaatccg cgactggcat gccggtagaa gccagtcagc
    16801 tctcagccag ctggcagaat tttggcccga cgcttgaagc acacgacatc cgtgcagaac
    16861 taaaggatgg cggcgaattt tcggttaaac gcgttactct tgcgctagat gtctggcaga
    16921 gcctgttaca tatgcgctgg cagtttcgcg acctcacttt ctggcagctg cgctttcgca
    16981 ccaacactcc tatcaccagc ggcggtggta atgatagcct ggaagccagt cacatcagcg
    17041 atctgtttct tcgtcagttc gaccattttg atctccgcga cagtgaagtc agtttcctga
    17101 cgccatccgg tcagcgcgcc gagctggcga tcccacaact cacctggctg aacgatccac
    17161 gtcgacaccg tgcagaaggc ctggtaagcc tctccagcct taccggacag cacggcgtga
    17221 tgcaggtgcg catggatttg cgcgatgatg aggggttgtt aagcaatggt cgcgtctggc
    17281 tccaggcgga tgacatcgac ctgaagccgt ggctcggtaa atggatgcag gacaatattg
    17341 cgctggaaac ggcacagttc tcccttgaag gctggatgac gatcgacaaa ggcgatgtaa
    17401 ccggcggtga cgtctggctg aaacagggcg gtgccagctg gttgggcgag aagcaaacgc
    17461 atacgctgtc ggtggataat ctgaccgcgc atattacgcg tgaaaatccg ggctggcagt
    17521 tctctattcc cgacacacgg atcacgatgg acggcaaacc ctggccgagc ggagcattga
    17581 cgctggcctg gataccggaa caggacgttg gcggcaaaga caataaacgc agtgacgaac
    17641 tccggattcg cgccagtaat ctggagctgg caggcctgga gggcatacgc ccgctggccg
    17701 cgaaactttc acctgcactg ggtgatgttt ggcgctccac acaaccgagc ggcaagatta
    17761 acactctggc gctggatatc ccgcttcagg cggcagacaa gacccgtttt caggcatcgt
    17821 ggagcgatct ggcctggaat caatggaaat tattaccggg tgcggaacac ttctccggga
    17881 cgctttccgg cagcgtagaa aatggtttgc ttaccgcgtc gatgaaacag gcaaagatgc
    17941 cttacgaaac ggtattccgt gcgccactgg aaatcgccga cggccaggca acgataagct
    18001 ggctgaacaa tgacaaaggt ttccagctgg atgggcgtaa tattgacgtt aaagccaaag
    18061 ccgtccatgc gcgcggcggt tttcgttacc tgcaaccagc taacgatgaa ccctggctgg
    18121 gtattctggc tggcatcagt accgatgatg gttcacaagc ctggcgctat ttcccggaaa
    18181 acttgatggg taaagacctg gttgattact taagtggcgc gattcagggc ggagaagcgg
    18241 ataacgcgac gctggtttat ggtggcaatc cgcaactctt cccctataaa cacaacgaag
    18301 gtcagtttga agtgctggtg ccgctgcgca acgcgaagtt tgccttccag ccggactggc
    18361 ctgcattaac taaccttgat attgaactgg actttatcaa cgacggttta tggatgaaaa
    18421 ccgatggcgt taatctgggc ggcgtgcgcg cgagtaatct caccgcagtg atccctgact
    18481 actcaaaaga aaaactgctg attgacgctg acattaaagg cccgggtaaa gccgttggcc
    18541 cttactttga tgagacaccg ctgaaagatt ctctgggtgc gaccctgcaa gaactccagc
    18601 tcgacggcga tgtgaatgct cgcttacatc ttgatatccc gctgaacggc gaactggtaa
    18661 ccgcgaaagg tgaagtgacg ctgcgtaata acagtctgtt tatcaaacca ctcgacagca
    18721 ccctgaaaaa tttgagcggg aaattcagct ttattaatgg cgatctgcaa agtgaaccac
    18781 tgacagcaag ctggtttaat cagccgttga acgtggattt ttccaccaaa gaaggggcaa
    18841 aagcctacca ggtcgcggtg aatctcaacg gtaactggca accagcgaaa accggcgttc
    18901 tgcctgaagc ggtgaacgaa gcattaaatg gcagcgtggc gtgggatggt aaagtgggca
    18961 ttgagttgcc ttatcatgct ggcgcgacct ataacgtaga gctgaacggc gatctgaaga
    19021 atgtgagcag tcacttacct tcaccgttag ccaaacctgc gggtgaacca ctaccggtaa
    19081 acgttaaggt tgatggcaat ctcaacagct ttgagttaac cggacaggct ggtgcagata
    19141 accatttcaa tagccgctgg ttgcttggtc aaaagctgac gctcgaccgt gctatttggg
    19201 cggcagacag taaaacgctc ccgccgttgc cggaacaaag tggcattgaa ctcaatatgc
    19261 cgccgatgaa tggtgccgag tggctggccc tgttccagaa aggcgcggcg gagagtgtcg
    19321 gtggtgcagc gagtttccca caacacataa cgttacgtac gcctatgttg tcgctgggaa
    19381 atcagcaatg gaataacctg agtattgttt cgcaaccgac ggcaaatggc accctggttg
    19441 aggcgcaagg gcgtgaaatc aacgccacgc tggcgatgcg taataacgcg ccgtggctgg
    19501 cgaatatcaa atatctttat tacaacccga gcgtggcgaa aactcgtggt gattcaacac
    19561 agtcatcacc tttcccgaca acggagcgca ttaacttccg tggctggccg gacgcccaaa
    19621 tacgatgcgc agagtgctgg ttctgggggc aaaaattcgg gcgcattgac agtgatatca
    19681 ccatttctgg caatacatta acgctgacca atggactgat tgatactggt ttctcgcggc
    19741 ttactgccga tggtgaatgg gttaacaatc cgggaaatga acgcacctcg ctgaaaggaa
    19801 aactgcgtgg gcagaaaatt gatgccgccg cagaattttt tggtgtcacg acgcccatac
    19861 gccagtcgtc atttaatgtg gattacgatt tacactggcg caaagcaccc tggcaaccag
    19921 atgaggcgac gttgaatggc atcattcata ctcaactggg caaaggcgaa attaccgaaa
    19981 tcaataccgg acatgccggg caattgctgc gcttattgag cgttgatgcc ctgatgcgta
    20041 agctgcgttt tgatttcaga gacacctttg gtgaagggtt ctattttgac tccattcgca
    20101 gcaccgcgtg gattaaagac ggcgttatgc acaccgacga cacgctggtg gatggcctgg
    20161 aagcggacat tgccatgaaa gggtcggtaa atctggtgcg tcgcgacctg aatatggaag
    20221 cggttgtcgc accagagatt tctgcgacgg tgggcgtggc tgcggctttt gcggttaacc
    20281 ccattgttgg cgcggcagtg tttgcagcca gtaaagtgct ggggccgctg tggagcaaag
    20341 tctccatttt gcgctatcac atttcgggtc cgctggacga tccgcaaatc aacgaagtgt
    20401 tgcgccaacc gcgtaaagaa aaagcgcaat gatttgacga gggcgcgtaa ttgccccaat
    20461 ctcatatgat aatcgttgcc aaaggccaac gagccggaac ataaccgtag gccggatagg
    20521 gcgttcacgc cgcatccggc agccgtaaaa aatcctctac tgcagtaact aacgagtagc
    20581 aaaaacgatg agtctgaacc tggtaagtga acaattgcta gcggcgaacg gcctgaaaca
    20641 tcaggacttg ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt atggcgatct
    20701 ctattttcag tcgagctatc acgaatcctg ggttttagaa gaccgcatta ttaaagatgg
    20761 ttcttacaac atcgatcagg gtgttggtgt gcgtgcaatc agcggtgaaa aaaccggatt
    20821 tgcttacgca gaccaaatca gcctgctggc gctggaacag agtgcgcaag cggcgcgcac
    20881 catcgtccgt gatagtggtg atggcaaagt acagacgctg ggcgcggtag agcatagccc
    20941 gttgtatacc tcggtagatc cgctgcaaag catgagccgt gaagagaagc tggatatcct
    21001 gcgtcgcgtc gataaggttg cccgcgaagc ggacaagcgc gtacaagaag tgactgccag
    21061 cctcagcggc gtttatgaat taattctggt tgcggccacc gacggcacgc tggcggcgga
    21121 tgttcgtccg ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg gcaaacgcga
    21181 acgcggtgcc agtggcggcg gcggtcgttt tggttatgag ttcttccttg ccgatctcga
    21241 cggtgaagtc cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc tggtcaatct
    21301 ttctgccgtt gctgcaccag cgggcaccat gccggtagta cttggcgcag gttggccggg
    21361 cgtgctgttg catgaagcgg taggtcacgg tctggaaggc gacttcaacc gccgtggcac
    21421 ttcagtattt agtggacagg tcggggagct ggtggcttca gaactgtgta ccgtggttga
    21481 tgacggtacg atggtcgatc gccgtggttc ggtggcgatt gatgacgaag gtacgccagg
    21541 ccagtacaac gtgctgattg agaacggcat cctgaaaggc tacatgcagg ataaactcaa
    21601 cgcgcgtttg atggggatga cgccgactgg caacggtcgc cgtgaatcct acgctcatct
    21661 gccgatgccg cgtatgacca acacctatat gctgccgggt aaatcgaccc cgcaagaaat
    21721 tattgaatcc gttgagtacg gtatctatgc accgaacttt ggtggcggtc aggtggatat
    21781 tacatccggc aaattcgttt tctccacttc agaagcgtat ctgattgaaa acggtaaagt
    21841 aacgaagccg gtgaaaggcg cgacgttgat tggttccggt atcgaaacca tgcagcagat
    21901 ttcgatggtt ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg gtaaagaagg
    21961 gcaaagtttg ccggttggcg tgggccagcc aacgctgaaa gttgataacc tgactgttgg
    22021 cggtaccgcg taataatttc agtattttca gaaggataat taatcctttt acgtgcacga
    22081 acggtcccct cgcccctctg ggattagggt gaggggaacc cgttggcaca ggtttgtaca
    22141 acgtaacagt ataatattaa ttacttctct ttcccgcgcc cgtgcatctc ctgaaacaat
    22201 ttaccgacct caacaaaata atccgttagc gagttgatca cgacttgtac cttcagcggc
    22261 agcttatctt tttcggtata taacgcataa accgggcgtg gatctgactg gtaacgtggc
    22321 agcaggatct ccagctcccc gcgattgatc tcgttgatca cccacatcag cggcacatag
    22381 gcgatcccag cgcctgcagt cagccagcgc accagcgtca tcggatcatt agtcacaaat
    22441 ctcccttgcg ggatcaggcg agtcgagatc ccttccggtg cgatcagttc aaattcattg
    22501 tcgggtcgca cgctgtattc aagccatgaa tgactactta aatcggcggg tttttccggt
    22561 atgccgtatt gtgtgagata gcttttcgcg gcgcacacca ccattggcat cgcgcccaga
    22621 cggcgggaaa acaggctgga atcctgcaac gcgccgacgc ggatcaccac atccagaccg
    22681 tcggcaatca ggtcgggggc aggaattccg gtaaccagat tgacgctcaa acctgggtat
    22741 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    22801 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    22861 tgcacttcat ggagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    22921 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    22981 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    23041 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    23101 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    23161 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    23221 gacagatgct gaaaataata acgcctgctc tctctataca accaaggtca acatgagtct
    23281 gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg atattttttg aattgctttt
    23341 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    23401 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    23461 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    23521 tcacggtcgt attagtcatt ctggccttca tcgcaatttt taatgcctgg gtctattaca
    23581 ccgaatcccc gtggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    23641 acgtttctgg acttatcacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    23701 aggtactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    23761 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    23821 gtctcggtgt gcaggcgatg tctcgcgaag agatcgatca ggccaacaac gtactacaaa
    23881 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    23941 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    24001 gtgagtttat tacccgtgga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    24061 tactggccta tatggaagaa actaagctgg aaggggtgcg ccctgggtat cgtgcagaga
    24121 tcacgccgct tggcagtaac aaagtgctaa aagggactgt tgatagtgtt gccgcagggg
    24181 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctaaccttg
    24241 aatgggtgcg tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    24301 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    24361 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    24421 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    24481 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcagt
    24541 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    24601 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    24661 cggactggtg atcattattg cgatgatccg tgcaccatta ttgatgattc tggtgtgctg
    24721 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtatgc
    24781 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    24841 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    24901 cattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtgg atcgagagct
    24961 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    25021 tgaagttgtc gataaagcct ggggcgatct ggtgcgacgc accacggcgt tacaaggtat
    25081 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    25141 gattaatacg ctatcgctga cgctgattac acaatcctgc gaaacttatc ttattcagaa
    25201 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    25261 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggactgggga
    25321 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    25381 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    25441 cgaaccggaa gtcaaagtag agtcagccga acgtcatcat gcgatggtta acttctggcg
    25501 aaccacactt tcctgcattc tggggacgct tttctggctg tggacgggct ggacttccgg
    25561 cagtggtgca atggtgatga ttgcggtagt gacgtcactg gcaatgcgtt tgccgaatcc
    25621 acgcatggtg gcgatcgact ttatctacgg gacgctggca gcgctgccgt tagggctgct
    25681 ctactttttg gtgattatcc ctaatactca acagagcatg ttgctgctgt gtattagcct
    25741 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    25801 ggcgctggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    25861 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    25921 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagttcgt
    25981 ttctgctgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    26041 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    26101 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    26161 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    26221 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aacttgaaat
    26281 ctatcaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    26341 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    26401 cgccaaaagc gtcggttttt tcatggctat acttagcgat gaacggcaga actcgccgcg
    26461 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    26521 aggaggattt ttatcgcgac ttcagccaag cctttggtct ggcgaaagat aaggtacgcg
    26581 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    26641 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    26701 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    26761 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    26821 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    26881 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    26941 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
    27001 gtcggca
//
LOCUS       DARNVM010000052        26899 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.052, whole genome shotgun sequence.
ACCESSION   DARNVM010000052 DARNVM010000000
VERSION     DARNVM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.052"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1585)
                     /gene="lsrK"
                     /locus_tag="SI621_001977"
     CDS             complement(<1..1585)
                     /gene="lsrK"
                     /locus_tag="SI621_001977"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HEI4064704.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYTLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAP"
     gene            complement(1664..2617)
                     /gene="lsrR"
                     /locus_tag="SI621_001978"
     CDS             complement(1664..2617)
                     /gene="lsrR"
                     /locus_tag="SI621_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HEI4064705.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEHETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRN"
     gene            2866..4401
                     /gene="lsrA"
                     /locus_tag="SI621_001979"
     CDS             2866..4401
                     /gene="lsrA"
                     /locus_tag="SI621_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HEI4064706.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFMNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDSATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4395..5423
                     /gene="lsrC"
                     /locus_tag="SI621_001980"
     CDS             4395..5423
                     /gene="lsrC"
                     /locus_tag="SI621_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HEI4064707.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5423..6415
                     /gene="lsrD"
                     /locus_tag="SI621_001981"
     CDS             5423..6415
                     /gene="lsrD"
                     /locus_tag="SI621_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HEI4064708.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGFPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6427..7449
                     /gene="lsrB"
                     /locus_tag="SI621_001982"
     CDS             6427..7449
                     /gene="lsrB"
                     /locus_tag="SI621_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HEI4064709.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADELLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7476..8351
                     /gene="lsrF"
                     /locus_tag="SI621_001983"
     CDS             7476..8351
                     /gene="lsrF"
                     /locus_tag="SI621_001983"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEI4064710.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8375..8665
                     /gene="lsrG"
                     /locus_tag="SI621_001984"
     CDS             8375..8665
                     /gene="lsrG"
                     /locus_tag="SI621_001984"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HEI4064711.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8722..9480
                     /gene="tam"
                     /locus_tag="SI621_001985"
     CDS             8722..9480
                     /gene="tam"
                     /locus_tag="SI621_001985"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEI4064712.1"
                     /translation="MSDWNPSLYLHFTAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9484..10398)
                     /locus_tag="SI621_001986"
     CDS             complement(9484..10398)
                     /locus_tag="SI621_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HEI4064713.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDETEIPAKILPDRHYQLT"
     gene            complement(10595..12046)
                     /gene="uxaB"
                     /locus_tag="SI621_001987"
     CDS             complement(10595..12046)
                     /gene="uxaB"
                     /locus_tag="SI621_001987"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEI4064714.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12273..13691)
                     /locus_tag="SI621_001988"
     CDS             complement(12273..13691)
                     /locus_tag="SI621_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEI4064715.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYALSYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVAFLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13830..14189)
                     /locus_tag="SI621_001989"
     CDS             complement(13830..14189)
                     /locus_tag="SI621_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEI4064716.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPTNDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14189..15115)
                     /gene="glsB"
                     /locus_tag="SI621_001990"
     CDS             complement(14189..15115)
                     /gene="glsB"
                     /locus_tag="SI621_001990"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HEI4064717.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15179..16567)
                     /gene="sad"
                     /locus_tag="SI621_001991"
     CDS             complement(15179..16567)
                     /gene="sad"
                     /locus_tag="SI621_001991"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HEI4064718.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANNSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16668..17543
                     /locus_tag="SI621_001992"
     CDS             16668..17543
                     /locus_tag="SI621_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4064719.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSIVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            17627..18742
                     /gene="yneK"
                     /locus_tag="SI621_001993"
     CDS             17627..18742
                     /gene="yneK"
                     /locus_tag="SI621_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HEI4064720.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNISSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKEDI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLHPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLTEQD
                     FPHFQDLFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRSPIPATAKTPDLPERH"
     gene            18892..20082
                     /gene="ydeA"
                     /locus_tag="SI621_001994"
     CDS             18892..20082
                     /gene="ydeA"
                     /locus_tag="SI621_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HEI4064721.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGAFITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20107..20772)
                     /gene="marC"
                     /locus_tag="SI621_001995"
     CDS             complement(20107..20772)
                     /gene="marC"
                     /locus_tag="SI621_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HEI4064722.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20984..21418
                     /gene="marR"
                     /locus_tag="SI621_001996"
     CDS             20984..21418
                     /gene="marR"
                     /locus_tag="SI621_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HEI4064723.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKRVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21439..21822
                     /gene="marA"
                     /locus_tag="SI621_001997"
     CDS             21439..21822
                     /gene="marA"
                     /locus_tag="SI621_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HEI4064724.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21854..22072
                     /gene="marB"
                     /locus_tag="SI621_001998"
     CDS             21854..22072
                     /gene="marB"
                     /locus_tag="SI621_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HEI4064725.1"
                     /translation="MKPLLSAIAAAFILFSTQGIAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22129..23568)
                     /locus_tag="SI621_001999"
     CDS             complement(22129..23568)
                     /locus_tag="SI621_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEI4064726.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLKFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23593..25266)
                     /locus_tag="SI621_002000"
     CDS             complement(23593..25266)
                     /locus_tag="SI621_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEI4064727.1"
                     /translation="MNIKTINVSLLSFSIITALFPLNALATKLTIEQRLELLENELSQ
                     NKQELRATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVVRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25322..25633)
                     /locus_tag="SI621_002001"
     CDS             complement(25322..25633)
                     /locus_tag="SI621_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEI4064728.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ELACLQAHKIQTALISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25661..>26899)
                     /locus_tag="SI621_002002"
     CDS             complement(25661..>26899)
                     /locus_tag="SI621_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEI4064729.1"
                     /translation="AAVRDGFVFTFPIIMASSLIILINFAILSPDGFIAGLLHLNSVF
                     PNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTP
                     YQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLARNPKITITMPAAVPPAVSRS
                     FKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYKLIQTPLKHMGTNIFAVIILGAVG
                     NFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTTWGAPYPITWTSINDAFANC
                     GGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFI
                     LVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVAT
                     MIYLPFVIAANKVNNLTTNG"
BASE COUNT         6920 a         6719 c         6409 g         6851 t
ORIGIN      
        1 caggcgcttt ccataacgac gtcgtcagcc cgtgatcaac cagccccagc tgatcctgat
       61 aaactgcctg ccacttatcg cgtgaatcct gataaagttc atgcttttcc gggtctggtg
      121 tgtgcgtccg ttcccagcga accaggcgtt ctccggtttc tgccattgat gaaaaaattc
      181 cggcaccgac gccagctgca atggcacatc ctaatgcggt ggcttctttg accaccggaa
      241 tattgacggg taatcccgag acatcagcga gaatttgact ccataatttc ccttttgaac
      301 ctccgcctgc aaagactaac gatgaaggat gaatattcga gaaatcagcg atttgctgca
      361 agttacacgc tgatacaatc gccgcatttt cttccagcgc acggaacaac gtcgctttgt
      421 tacatttatc cggatcaata gacaaattaa taaaggaagg cgcagcgtga taccaggttt
      481 taaagcgcat tctgtcggag aatatcggca ttacgcccca cgacccaggc ggcacccgac
      541 ttgccatctc ttccagcagc gtataggtgt cgatgcctaa acgttcggca atcagttttt
      601 cttcggcaca gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa aagcttatag
      661 attcagcttg taccatgcca ggaataacat gaggattaac gcgcacgttc atttctgggt
      721 ctgtcaccgg cgcggctaag tttacaactt gctgccagaa tgtgccgcca agaacagcgg
      781 tttgtgccgg acgcacaacg cctaacccca ggcaaccaag ttgcacgtcg cctcctccaa
      841 cgaccaccgg agtgcccgcc ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc
      901 ccagcaatgt gccggtttct ttgacaggag aaagaatatc ggcccgtagt ccggccatat
      961 ccagcaatgc aggtttccag tcacgggtgg ttagatcaag aagtcccgtg gtgccagcgt
     1021 tagagggatc caccgccagt tcgccgctga gcgtataggc cagccagtcg ctgatcatgg
     1081 tgatggtcga cgcctgtcgg tagatatcag aacgatggtg cgccagccaa agtaatctgg
     1141 gaatagcgct taaagccagc gtttgtccgg tggcgcgata aacttcgttt tcaaaggtat
     1201 tgttgtgcag ttctttaagt tcgctaactt cgcgcgccgc tctggcatcc acattggcgc
     1261 aggcccagat cggtgttcct tcattgttat ataaaacaat gccttcgcgc atcgaacatg
     1321 ccgaaacggc agcgatatac tccggggcta tgccggcgtt gtgcagcgcc tggcgcatac
     1381 actcacacgc cagttgccag tttttgttga gatcaaattc catagaacca ggaacgtccg
     1441 gtactgccag atgccgccac tccgcctgtc ccactgctat ttgattgcct tccaggtcga
     1501 atatcacagc ccgaatactt ccggtgcctg catccagcgc catcaggtag tactttgatt
     1561 ctgaaggggt aaagagtcga gccattagat tatcctcggc ttatgcgttc tgtctgttcc
     1621 tctatacgtt ctccatcatt cccggtaata aggtcatgca aatttaatta cgtaaaatcg
     1681 ccgctgctgt gtcctgatcg gtaaccagtg cgttgatata accgcctttc attgcagcgg
     1741 caatggcttc ggctttattt tctccccctg ccacgccaac ccggacgggt atggtcttca
     1801 gcgcgcttaa aggtaagcca atcagttcgt tatgtatttt aatatccgtg acaacgtcac
     1861 cttttgcatc aaaaaagtag cctaaaatgt cgccaacagc cccttttcga ccaatcatta
     1921 actgttcgcc ctggctgata tagccggagc gaatgatcgt cgcgtcgtct tgttgactca
     1981 cggccccaat gccgacaatc gccacatccg cagcttgtgc ggctaacaga acatctttga
     2041 cgcaattttc attttttagc gtacgggcaa tgtcagcgga ggatgcccgc agcggagccg
     2101 gaataatatt cacactgcac gccgcattaa gctgcccgat acccgtcata taagaaccga
     2161 cgccaccgga gagcgtgacc aggcgaattt gctgcgacga aataaaacca cttaagcgtt
     2221 gcagcgtatt catggttgcc tcgccaaaac caatcgccag catctgttgt ggttgaagta
     2281 aactcatcaa catatgcgcc gcgcctatcc ctagtcgccc gccgacatca gcatccgcaa
     2341 gcccagggat cacccggaca tgttgcagcg aaaactgacg acgtaattgt gtttcatgtt
     2401 ccagacagcc ttcaaagcga gaattaatct gcacgcgaat aatgccggac tgatgccctt
     2461 tttccagtaa tcgcgacact ttcaagcgag tcagcccgag acgatcgctg atctcgctct
     2521 gggtcagccc gtcgtggtag taaaaccacg cgatccgcgc gacctgttct tcttcacaca
     2581 ttccctgttc tgaaattgcc gaatcgttga ttgtcataat tcattcttca ctttgaacat
     2641 atttaaatct ttaatgcaat tgttcagttc ttgcgcattt atatctgtga tggcaaccac
     2701 agtttgactc tacgagtatg aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg
     2761 atctattcgt cggaaatatg tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga
     2821 aatacatttg ttcaaaactc acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat
     2881 acccgcgcgt taccgctact ttgcgcccgc tcggtttata aacagtattc aggggtcaat
     2941 gtcctgaaag gcatcgattt tacgttgcat cagggggagg tccacgccct gctcggcggc
     3001 aatggtgccg gtaaatcgac gttaatgaag attattgccg gtattacccc tgctgatagc
     3061 ggtacgctgg agattggggg caacaactac gccagattaa cgccagttca tgctcatcag
     3121 ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa
     3181 aacatcctgt ttgggctggc aaaaaaacag ctctccatgc agaaaatgaa gaacttgctg
     3241 gcggcgctgg gctgccagtt tgatctgcat agtctggcag gatcgctgga tgtcgccgat
     3301 cgccaaatgg tggaaatcct ccgcgggctg atgcgcgact cgcggattct gatcctcgat
     3361 gaacctaccg cctcgcttac ccctgcggaa accgaacgct tgtttactcg cttgcaagag
     3421 ctgcttgcta ctggcgtggg tattgttttt atctcgcata agctgccgga aattcgccag
     3481 attgccgatc ggattagcgt gatgcgcgac ggaactatcg ccttaagcgg caaaaccagc
     3541 gaactgtcta ccgacgacat tattcaggcc atcacgccag cgctacggga aaaatcgctc
     3601 tctgccagcc aaaaattatg gctggagtta cctggcaacc gcccacaaca tgccgccgga
     3661 acgccggtgc tgacactgga aaacctgacc ggcgaaggtt ttatgaatgt cagcctgacg
     3721 cttaatgccg gagaaattct gggtctggct gggctggtgg gggccggacg cacagaactg
     3781 gccgagacgc tctatggtct gcgtactttg cgtggcggac gcattatgct gaatggtaaa
     3841 gagatcaata aattatccac tggagaacgt ttactgcgcg gtctggttta tctgccggaa
     3901 gatcgccagt catccggact gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt
     3961 actcataacc ttcgtggatt ctgggcgaaa accgcgaaag atagcgccac actggaacgt
     4021 tatcgtcggg cgctgaatat taaatttaac caaccggaac aagctgcgcg gaccttatcc
     4081 ggcggcaacc agcaaaaaat cctgattgcc aaatgtctgg aagcctcgcc gcaagtattg
     4141 attgtcgatg agccgacgcg cggcgtggat gtctcagccc gtaatgatat ctaccagctg
     4201 ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta tctcctctga tctggaagag
     4261 atcgaactga tggcagatcg cgtatatgtg atgcatcagg gcgaaattgc ccactctgca
     4321 ctgaccggac gcgatattaa tgtcgagacc attatgcgcg ttgccttcgg cgatagtcag
     4381 cgtcaggagg cgtcatgctg aaatttattc agaacaaccg tgaaatcaca gcactgctgg
     4441 cggtggtgct gctgtttgta ttacccggtt ttctcgaccg ccagtattta agtgtgcaaa
     4501 cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct gctggcaatg ggcgcgacgc
     4561 tggtaatgct tacgcgcaat attgatgttt cagtgggttc gattaccgga atgtgcgcgg
     4621 tgctgttggg gatgttactg aacgcggggt attcactacc tgttgcttgt gtcgcgactc
     4681 tactgcttgg tttgctcgcg ggatttttca acggtgtcct ggtcgcgtgg ctaaagatcc
     4741 ctgccattgt tgccaccctt ggaacgttag ggttgtacag aggcatcatg ttgctgtgga
     4801 ctggcggtaa atggattgag ggattacccg ccgaactgaa acaactctcc gccccgctgc
     4861 tgtttggcgt ttccgcgatt ggttggttga cgataattct ggtggcattt atggcctggc
     4921 tgctggcaaa gacggcgttt ggacgcagtt tttatgtcac gggcgataat ttacagggcg
     4981 ctcgtcaact gggcgttcgt acggaagcca ttcgcattgt ggcattttcg ttgaacggct
     5041 gcatggcggc actggcggga attgtgtttg cttcgcagat tggttttatc cccaaccaga
     5101 ccggtaccgg gctggagatg aaagcaattg cagcctgcgt gctgggcggc attagtttgc
     5161 tcggcggttc cggcgcgatc atcggtgcgg tactcggcgc atggttcctg acgcagatcg
     5221 atagtgtact ggtgctgttg cgcattccgg catggtggaa cgattttatc gcgggcctcg
     5281 tgctgctggc ggtgctggtg tttgatggac gcctgcgttg tgcgctggaa cgtaatctgc
     5341 ggcggcaaaa atatgcccgc tttatgacgc caccgccatc cgttaaaccc gcttcgtcag
     5401 gtaaaaaacg ggaggctgca taatgcgtat tcgctacggt tgggaactgg ctcttgccgc
     5461 actgctcgtt attgagattg tcgcatttgg tgcaattaac ccgcggatgt tagatctcaa
     5521 tatgttgctg ttcagcacca gtgactttat ctgcattggc attgtcgccc taccgctgac
     5581 gatggtgatt gtcagtggtg ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc
     5641 cattgcattg ggcgtattgt ttcaaagtgg tgtgccgatg ccgctggcga ttctcctgac
     5701 cttactgctc ggcgcattgt gcgggctgat caacgccgga ttaattatct ataccaaagt
     5761 taacccgctg gtgattacgc ttggcacgct gtatctgttt gccggaagcg ctctgctgct
     5821 ttccgggatg gccggagcga cggggtacga aggtattggt ggattcccga tggcgtttac
     5881 agatttcgct aacctggatg tgctgggatt ccccgttccg ctgattatct ttctgatatg
     5941 tctcctcgtt ttctggctct ggctgcataa aacccatgcc ggacgtaacg tgtttttgat
     6001 tgggcaaagc ccgcgcgtgg cgctttatag cgcgattcca gttaaccgca ccttatgtgc
     6061 gctctatgcc atgacggggc tggcgtctgc agtcgccgct gtgctgctgg tatcgtattt
     6121 tggttcagca cgttccgatc tcggtgcgtc gtttctgatg cccgccatca ccgccgtggt
     6181 gcttggcggt gccaatattt atggtggttc cggttccatt atcggcaccg ccattgcggt
     6241 tttattagtg ggatatttgc aacaaggttt gcaaatggca ggagtgccaa atcaggtgtc
     6301 cagcgccctt tccggtgcgc tacttatcgt cgttgtcgta ggtcgttccg ttagcctgca
     6361 tcgccagcaa attaaagagt ggctggcgcg tcgggccaat aacccattgc cataaaggat
     6421 atcttcatga cacttcatcg ctttaagaaa atcgccttac ttagcgcgct tggcattgcc
     6481 gcaatctcta tgaatgtgca ggccgcagag cgtattgcat ttattcccaa actggttggc
     6541 gtgggatttt ttaccagcgg tggcaacggc gcacaacaag cgggtaaaga gctgggcgtt
     6601 gatgtgacct acgacgggcc gacagaaccc agtgtttctg gtcaggtaca gttgattaat
     6661 aacttcgtta atcagggtta taacgccatt atcgtttctg cggtttcgcc tgatggcttg
     6721 tgtccggcac tgaaacgcgc catgcaacgt ggtgtgagag tgctgacctg ggactctgat
     6781 actaaaccgg agtgccgctc ttactacatt aatcagggaa cgcccgccca gttgggaggt
     6841 atgttggtgg atatggcggc gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc
     6901 tactcaagcc ccaccgttac ggaccaaaac cagtgggtga aagaggcgaa agcgaaaatc
     6961 gccaaagagc atccaggctg ggaaattgtc actacgcagt ttggctataa cgatgccact
     7021 aaatcgttac aaactgcaga aggaatatta aaagcctata gcgatctcga cgccattatc
     7081 gcccccgacg ccaacgccct gcccgctgcc gcacaagctg cagaaaacct gaaaaatgac
     7141 aaagttgcga ttgtcggatt cagtacgcca aacgtgatgc gtccgtatgt ggaacgcggc
     7201 acggtgaaag aatttggcct gtgggatgtg gttcagcaag gtaaaatatc agtttatgtc
     7261 gcggatgaat tattgaaaaa aggatcaatg aaaacgggcg acaagctgga tattcagggc
     7321 gtaggtcagg tggaagtctc gccaaacagc gttcagggct atgactacga agcggatggt
     7381 aatggcatcg tactgttacc ggagcgcgtg atattcaaca aagagaatat cggcaaatac
     7441 gatttctgat gtgcattact taaccggagt acgttatggc agatttagac gatattaaag
     7501 atggtaaaga ttttcgtacc gatcaaccgc aacaaaatat cccttttacc ctgaaaggtt
     7561 gcggtgcgct ggattgggga atgcagtcac gcttatcacg gatatttaat ccgaaaacgg
     7621 gtaacaccgt gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg
     7681 aacgcattga tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc
     7741 gcggcatttt gcgcagcgta gtccccccgg cgactaataa gccagtggta ctgcgggcgt
     7801 caggtgcgaa ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg
     7861 acgccgtgcg cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agtgaatatg
     7921 aacatcagtc gattaaaaat attattcagc tggtcgatgc cggaatgaaa gtaggaatgc
     7981 cgaccatggc cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc
     8041 tggctacccg aatcgccgct gaaatggggg cgcagattat caaaacttat tatgtcgata
     8101 aaggttttga acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa
     8161 aattaccgga gcgcgaggcg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg
     8221 gtgtggatat ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg
     8281 tacaggcggt ggtacaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg
     8341 aaaaacagta actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa
     8401 cgttcatgaa gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc
     8461 ggtacaggaa gaaggcaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg
     8521 cttttttatc tacgaagcct ataaagatga agacgcagtg gcgttccata aaaccacgcc
     8581 ccactacaaa acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg
     8641 tctgttcaat gggttgatgc cgtgaggcga atttatcaat tttatctaca attggggtaa
     8701 cgcgctgacg ggagtaaaaa aatgtctgac tggaacccct ctttatatct gcactttacc
     8761 gctgaacgat cgcgtccggc ggtggagctg cttgccagag tgccgctgga aaatatcgaa
     8821 tacgttgccg atcttggctg tggcccaggt aacagcaccg ccctgctgca tcaacgttgg
     8881 cctgcggcca ggataacagg catcgactcg tctccggcga tgattgctga agcgcgcagt
     8941 gctttgccag actgccagtt cgtggaagca gacatccgca actggcaacc ggaacaggca
     9001 ctcgatctga tttttgctaa tgcctcactg caatggctgc ccgaccacta cgaattgttc
     9061 cctcatctgg tttctttact taatccgcag ggtgtgttgg cagtacagat gccagataac
     9121 tggctagagc cgacccatgt gctcatgcgc gaagttgcct gggaacaaaa ttatccggat
     9181 cgcgggcgtg agccgctggc tggcgttcat gcttactacg atattttgag cgaagccgga
     9241 tgtgaggtcg atatctggcg aaccacctac tatcaccaga tgccgtcgca ccaggcgatt
     9301 atcgattggg tgaccgccac cggattacgt ccgtggttac aggatctgac cgagagcgaa
     9361 cagcagcatt ttcttacgcg ctaccatcag atgctggaag agcagtatcc actgcaagag
     9421 aacggacaga tactgctggc atttccgcgt ctttttattg ttgcccggcg tacggagtaa
     9481 atattacgtc agctggtaat gacgatcggg aagaattttt gctggaattt ctgtttcatc
     9541 gttcatctgt aacaggtcaa tttcaatagc gttgcagata gcatccagcg gtaaatcatt
     9601 gttttcagta ccgaagggat cttccagttc ttccgccaga caatccagcg aaataaaagt
     9661 gtaggaaatc agcacagaga taaaaggcgt catgtaatgc aggtccacga ccagcgcgaa
     9721 cggcagcatg atacaaaaca gataaacggt gcgatgcaaa atcagggtgt aggcaaaggg
     9781 aattggcgta taggcaatgc gctcgcagcc tgccagaacc gctgaaatat cgttaagccg
     9841 atcgttgagg ctaataaaca ggatatctga aagctgtcca ttgcggcgct gaaccgccaa
     9901 ccattctccc attattaaca agatacggtt agctggagag ttcgaagcca gtacacgctg
     9961 aagatcttca gtcttgagat aatgtgccag cacttccgcc tgtggctgtt tgcgtaatgt
    10021 catgcgtaaa caatgggcga aggcaatttg cagccgggca aactccctta cacttgccga
    10081 atccggcaat gtcgttttta cctcccgcag taacgaccgt gaggcaatca tcaactgccc
    10141 ccaaagtttt cgcgcttcaa cgtaacgggc gtacccggca ttattacgaa aaccaagaaa
    10201 aatggcgatg gcaacaccga gaatgctgaa cggtgcgagg gtgaatttga tgcccaaatg
    10261 cgtgtaccag ggcagcatga aaataacagc gatagaaaag agaaaattga gtagtaagcg
    10321 cgaggatatc ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg
    10381 ttgtggacga acaatcatgg ttatcttcag gcgtggaaaa gtcgccctat taaacgtgat
    10441 tacgatcaca ttctcaagac gctacgtaca aattacctac aagcttacaa catttacagg
    10501 tgtggcatat ggcacccgtt aatgataaag gcacatagga tgttgtaact aatatagtaa
    10561 aataagaaac ccggtcgaaa ccgggttcag agtattagca caacggacgt acagcttcgc
    10621 gcattccttt ttcgagaatc gcatccaggt cattagccac ctgctcgacc agaccaggca
    10681 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa aacaatcgct accagttcct
    10741 gggtaccgat aacacggtca cgatgctggc tccagagttg ctgataacgt tccagccaat
    10801 gtgcatcgtc ctgtaccgga tatgtttctc cattacgctc accgcgataa aacgcaatca
    10861 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
    10921 gcagttgcgg caggatgcgg gtgcggaact tggtcatacc gttgagggcg atagatagca
    10981 actgatgttt gatgtacggg ttacggaaac gtccggtgac tgcactggcg aaagactcca
    11041 gctcatcgcg tggcaaatcc agtaccggaa taatttcttc gtagatggct ttttcaacga
    11101 atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt atccagccct gcctggaacg
    11161 ctaccggcac cagcgcggtg tgcgcaccgt tgagtattgc cactttgcgt tctttgtacg
    11221 gtttaatatc gtcaacaatc agcacgttga gcggatattt gtccagacgc agttcagtcg
    11281 ctaaggattt cggcccctga atcacaaaca ggtaaaagtg ttcagcggtg tcgaggaaac
    11341 catcgtgata acccagttct tcttccagtt tagccacttc atcacgcgga tagccggtaa
    11401 cgatacggtc caccagcgta gaacagaagc tgttagcctg atccagccat tgaataaatg
    11461 cttctggtag tgcccactct tgcgcatagc gcagcacaag ttcacgcaac gcgtcgccat
    11521 tgtagtcgat caactcacac gggatgatga tccaaccttt atccagcgca ccgttgaaat
    11581 ggctaaaacg ttcgaacagc agacgggtca gttttgccgg ataacttacc gctggcgcgt
    11641 catcgaattt atcgcccgca tggtagctga tacccgcttc ggtggtgtta gagaaaacaa
    11701 agcgcatttc cgggttatgc gccagtttca ggaattcatc gtattcactg tagacgctga
    11761 tttcacgatt aacagagcga atcagacgcg cgtcgctgac cgcttccccc ttctcgttca
    11821 gaccacggat aatggtggta tacagaccat cctgcgtgct cagtgacggc gggaatgaag
    11881 tttcaatcgg acgaacaacg accacgccag aattcagatc ggtgtgctca ttcaggagat
    11941 cgatttgcca gtcaacaaag gcgcgcagga agttaccttc accaaactga atgatacgtt
    12001 ctggatactg tgcaccggga aaatcgcgac gatttagtgt tttcacaatg ggttcccttc
    12061 tgattagtca tacaacctgt ttgaattggt acgacaggtt agcaaacttt aatacgccga
    12121 acccctgttt tgatcaactc ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc
    12181 ggatcatggt ttaatcgagg aaaaacgcct tttcttggat cataaagtgg tagaacacat
    12241 tccgttaaaa tcgctgtgaa tgaataaaga tgtcagacaa cttcctcacc gtaacgcata
    12301 gtgctggtac ggttgcgccc atctttcttc gagcgataca gacatgtatc ggcttcgacc
    12361 atcaatttat taaagtcatc ggtcagcgtt cggtacgatg cgcaaccact accgacgcca
    12421 atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct
    12481 ttacggattt tttccgccat tagcagacca tcgacaggat tcaccgacgg cactgcaaca
    12541 gcaaattctt cgccgcccat tcgtgccacc agccccttat caccgacaat cttctgaata
    12601 tgccttgcaa acacgcttaa cactttatcg ccacattcat gtccatagtt atcgttaata
    12661 cttttgaaat agtcgatatc aagcagcatg acagtcagat gttgtgtctg tttcagcgat
    12721 ggacttttca gcgcctcata aagaccggac cgtgagtaaa cctgagtcag aaaatcaaag
    12781 tcggctcgca gcgcaacttg cttcattagc gaattgatcg ctgccacgct aaaagaaacc
    12841 ataattgggc atatcgccat cgtagcaata ccgagacgtg cggagaacat ttccggaatg
    12901 gagaacggcg aaccgaccga gatatcaatc accgaatttg ccaccagtac gatttccacc
    12961 gcaccggtga caaaggtcaa cagacatgtt acctgcggcg tatagcgcac ggcacaccag
    13021 attaatgcag gcagcgggaa cgccagactc cccgctccgc caatgactac cgaggcaata
    13081 accgacacaa taagcgcgat ggcgggcatc atctgttctg ctttaaagcg cggcagcact
    13141 cccggaatag ccaacgtcag catacaaggc acgatcaaca cgcccgttga gaattgctca
    13201 ctgaaccagt ccgcaagcaa aggccagaaa tccagactat caatactgac cgaaccaatc
    13261 gccccgacaa tagcgcataa taatgcggca atcagacagt aattaaatag ccgtaaggca
    13321 cttactggct catacttatt tttccccaga cgcttatccc gcgcgaccag aaaggcgacg
    13381 gtaacaataa acatcatatt ggagaaattg atagccagtg aaaccagccc ccattcggtg
    13441 gttatggcat cataaaccag catcgccaca taactgagcg catagtaatg cagccgatta
    13501 agccagacat agcgggcaaa cacccccgcc attacgccat tcagcggcca gaaaagagaa
    13561 agttcgtgaa ccagccgtaa ctctgcacca atgaaataga acagcgtggt tagcgcaaaa
    13621 atagcgatgc tattacgtag cagatgacct tcctggaaca aacggaaagt tgaaatgggc
    13681 tgtacatgca ttaattatct gtccatacaa ttgcgggtgg caattatctc aacgtaataa
    13741 gtaaattctt ataaaggata gcatgttcgc tgacagcggc ggcaaataaa gatatcctgg
    13801 gaatgctatt ttatcgtttt ccgcaattat catggctgat tcatctgtat aaccaaccag
    13861 tgatagatta ccgccacgat ataacgttgt tgttcctcgc ttaacgaaac accctgcgga
    13921 atgttatgcc acttagccag acaaccgcga caacaggttg cagtggcgtg ctgagcgata
    13981 aacacaggat gcccgcgcat gggggtttgc ttaccgtcat tagtcggtaa tgccggagca
    14041 agacgtttag cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc tttctccaga
    14101 caatactgac gctctttcat gcccaaacga aaccgagagc gaaattttga acgggataaa
    14161 cgcgcgaaga gtggatcgag agactgcatt aataaaccga acgccctagt tgtttcgtca
    14221 attgttcaag gacagcaatg cccgcaagcg agttaccggc atcatccagt tccgggctcc
    14281 agactgcgat ggccatttca tgcggaacaa tcgccacaat accgccacca acgccagatt
    14341 tcgccggtag tcccacccgc caggcaaact cccccgcgtt ctggtacata ccactggtcg
    14401 ccatcagtgc gttaatttgc cgcgcctgca ttggcgtcac cacaggttca tcaatatgaa
    14461 tagctttccc ctgattagcc agaaagacaa acgtccgggc cagctctaca cagctcattt
    14521 tcagagcgca gtaatgaaag tagttttgca gaacggttgt cacatcatga tggaaattgc
    14581 caaacgactt catcagccag gcgatagccg catttcgcgc ggagtgttca aattcggaac
    14641 gcgctaccac cgtatcgtat gaaatatcag acacaccgct taagccgcgc acgacttcca
    14701 gcatacgttg ccgaggagcg cttaatcgcc cttgcaacat atcgcagacc accagcgcac
    14761 cggcattaat aaacggatta cgcggaatac cctgctccat ttccagttgc actaaagaat
    14821 tgaacggtga tccagacgga tctttgccga cgcgttgcca gatttcctct tcggagtaat
    14881 gacgcatggc gacaacgaga ctcagcactt tggaaataga ctgaatggaa aaacgttctt
    14941 gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca gatagcaata cccagccggg
    15001 aaccgtctac cgtagccagc gccggaatat aatccgcgac tttaccctga ccaatgagcg
    15061 gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc catggcgact gccactttct
    15121 actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctgaat atgtagcgtc
    15181 agatccggtc tttccacacc gtctggatat tacagaattc gtgtaagccg aaatgggaaa
    15241 gctcacgacc aaagccactc tttttcacgc caccaaaggc cactcgcgcg tcgctggcac
    15301 aataaccatt gataaacacc ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg
    15361 tttcgtcggt ggtgaaaatg gtcgctgaaa ggccgaactc gctattatta gccagttcca
    15421 gtgcatgttc tgcatctttc gcaatggtga ttgccgcaac ggggccaaac atttcttccc
    15481 gaaacgcggt catttctggg gtaacattcg ccagaaccgt tggcggatag tagttacctg
    15541 ccccagccat cttttccccg cccagtaaca aacgcgcacc ctgcgccagg gttttctcca
    15601 cctgatgatg cagctcatca cgtaaatcaa aacgagccat tggtccgaga gtgttctctt
    15661 cgtcacgggg atcgcccatt ttcaaggctg ccgcagctgc cacaaaacgt tcggtaaatg
    15721 ccgaagcaat tccctcttcg atgataaagc gttttgccgc tgcacaaacc tgtccggtat
    15781 tctgataacg tccggctacc gccgctttaa ccgccagttc cagatcggca tcgttaagca
    15841 caataaatgg atccgaaccg cccagttcca gtacgcattt tttcagtgcc gctccagcct
    15901 gtgcgccaat agccgctccc gcacgaacac ttccggtcac cgtgacagca gcaatgcgcg
    15961 agtctttaat catctgactg acgccgtcgt tgtcggcatt cagccagcca tatacgcctt
    16021 gtgggatacc cgcatcttta aagacctggg caatgagctg tgcacagccc atcacattcg
    16081 gcgcatgttt aagtaagtag ccgttacctg caagaatgat aggaaccgcg ccacgcatca
    16141 cctgccataa cggaaaattc cacggcataa tcgccagaat tgtccccaac ggtcgatact
    16201 caataactgc ctgctgattt tccaccagcg taggttccgc cttcagcatt gccggaccat
    16261 gttctgcata ccagtcacac aaattcgccg atttcgccac ttcagcgcgc gcctggttga
    16321 ttggtttgcc catttcacgg gtgatcattt gcgccatttc ttcgctacgg acacgcagag
    16381 ccttaccgat atcacgcagt ttttcagcac gataatctat atttgtctcg cgccagtcgc
    16441 gaaagcctgc tgccgccagc tgaagtgcgt tttctatatc gtcagcgcca gcccacggca
    16501 gcacagaaag ttgttcaccg gtggcaggat ttatcgaaat tgcatgagtt gccggagtaa
    16561 tggtcatcgg ggtatctcct ttatgagtta tggtaaaaag atacgcagat ttactcttgc
    16621 ttgaaaatga ataatattaa gccacttatt catgaatcga gaatattatg gatctgaccc
    16681 aactggagat gttcaacgcg gttgccgaag ctggcagcat tacccaggct gcagcaaaag
    16741 tgcatcgcgt gccgtcgaat ctcactaccc gtctacgcca gctggaaaca gaactggggg
    16801 ttgatctgtt tattcgcgag aatcagcgtt tacgtctctc gcctgccggg cataactttt
    16861 tacgctatag ccaacaaatt ctcgcattag tggatgaagc gcggagcatt gtcgctggcg
    16921 atgagccgca aggtttattt tctcttggtt cgctggaaag caccgcagca gtgcgcattc
    16981 cggccacgct ggcggaattt aaccgtcgtt atcccaaaat tcagttttca ctttccaccg
    17041 gcccttccgg cactatgctg gaaggggtgc tggagggaaa actgaatgcg gcgtttattg
    17101 atgggcccat taaccatact gccattgacg ggataccggt gtaccgcgag gagctgatga
    17161 ttgtcacgcc acaaggacat gcgccagtaa cccgtgccag tcaggttaat ggcagtaaca
    17221 tttatgcctt ccgcgccaat tgttcgtatc gtcgccactt cgagagctgg tttcatgctg
    17281 acggtgccgc tccgggaact attcacgaga tggagtcgta tcacgggatg ttggcctgtg
    17341 tggtcgcagg agcaggcatt gcgcttattc cacgctctat gctggaaagt atgccggggc
    17401 atcaccaggt tgaagcgtgg ccgttagctg agcaatggcg ttggttaaca acctggctgg
    17461 tctggcgtcg cggtgcgaaa acgcgtccgc ttgaggcatt tattcaacta ctggatgtgt
    17521 ctgactcggc aaaacagagt tagcaatgag ctatttttga tagttcttgc ggctaatatg
    17581 ctctatatag tgatgctccg atgacttatg actatatggg gcaaatatgg ttacgccagt
    17641 aagcatcagc aattacatat ctcttcctga tgattttccc gttcgtaata ttgcacctca
    17701 ggtaaaagaa gttttaaagg attttattga tgcgctaagt acaataatat gtgatgaaga
    17761 gtggcgtacg tctttaaaca tcagctcagc cacaaaaaag atatttaata accttgataa
    17821 cttgtcatat attcagagaa cctctttccg gggtaacgac acgctataca atgaaaaagt
    17881 tcaatttaaa ctcacttatc ctgccaggaa tggaagacac aaagaagata ttgaatttca
    17941 ggtagtaata aatttaagcc ctatttattt agataatttc cgccatgatg gggaaattaa
    18001 tattttttgc gctcccaacc caaagcctgt cactatgggg cgcgtatttc agactggcgt
    18061 cgagcgtgtg ctctttctgt ttctgaatga ctttatagaa caatttccaa tgatcaaccc
    18121 tggcgttcct atcaaaagag cgcatacgcc acatattgaa cccctgcatc cggatcacca
    18181 taccgctgca gattacttac gccagtttga tttgcttgtc ctgaatttta tctctcgcgg
    18241 taattttgtc atactccccc gattatggaa taactctgag gttcacagat ggttcgggaa
    18301 taaagaacct aacttgctca ccgctattct cgacataacg gacagcgaat taaaagagga
    18361 tttattgcaa agtctgatgg attcactagg ttctaacaaa catgtactac cagaagtctg
    18421 catctgcttt ttatcccttt taacagaaca agattttcca cattttcagg acttgttttt
    18481 atttttcgcc aatatgttat tgcactatca ccaatttatg aatcccaatg aaagtgattt
    18541 gattgacgtg ttgatgccag catcattaag tgatgataaa attatcaaac atatggcgcg
    18601 caggaccctc aaactttttg taaaaaatga aacgccgcca aaagttactc acgaagacct
    18661 ggtgaaaaac aggcctcgct cccctgtcag atcacctata cccgcaaccg ccaaaacgcc
    18721 agacctccct gaacgtcatt aaaccgtgat gttaccgact ctctgacgcg gaacagaatc
    18781 agcgtcagag aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa
    18841 ccgttttaag atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca
    18901 aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc
    18961 atcttcaaca ccaccgaatt tgtcccagtt ggcctgctct ctgacattgc gcaaagtttt
    19021 cacatgcaaa ccgctcaggt cggcattatg ttgaccattt acgcatgggt agtagcgcta
    19081 atgtcattgc cttttatgtt aatgaccagc caggttgaac ggcgcaaatt actgatctgc
    19141 ctgtttgtgg tatttattgc cagccacgta ctgtcgtttt tgtcgtggag ctttaccgtt
    19201 ctggtgatca gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg
    19261 tctctggcga tccgtatggc tccggccggg aagcgagcgc aggcgttgag tttaattgcc
    19321 accggtacag cactggcaat ggtattaggt ttacctctcg ggcgcattgt ggggcagtat
    19381 tttggctggc gaatgacctt cttcgcgatt ggtatcgggg cgtttattac ccttttgtgc
    19441 ctgattaagt tacttccctt gctgcccagt gagcattccg gttcattgaa aagcctcccg
    19501 ctattgttcc gccgcccggc attaatgagc atttatttgt taactgtggt ggttgtcacc
    19561 gcccattaca cggcatacag ctatatcgag ccttttgtgc aaaacattgc gggattcagc
    19621 gccaactttg ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgtg
    19681 attttcggga aactgggtaa tcagtatgcg tctgcgttgg tgagtacggc gattgcgttg
    19741 ttgctggtgt gcctcgcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg
    19801 ctaagtattt tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg
    19861 ctggcgctgg caccagatgc caccgacgtc gcgatggcgc tattctcggg gatatttaat
    19921 attggaatcg gcgcgggtgc gttggtaggt aatcaggtaa gtctgcactg gtcaatgtcg
    19981 atgattggtt atgtgggcgc ggtgcctgcc tttgccgcgt taatatggtc aatcatcatt
    20041 ttccgccgct ggccagtgac actcgaagaa cagacacaat agttgaaagg cccatttggg
    20101 ccttttttag tggtacgttt taatgatttc caggatgccg ttaataataa actgcacccc
    20161 catacatacc agcaggaatc ccatcagacg ggagatcgct tcaatgcccc ccttacccac
    20221 cagccgcata attgcgccag agctgcgtag acttccccac aaaataaccg ccaccaggaa
    20281 aaagatcagc ggcggcgcaa ccatcagcac ccaatcagcg aaggttgaac tctgacgcac
    20341 tgtggacgcc gagctaataa tcattgcaat ggttcccgga ccggcggtac ttggcattgc
    20401 cagtggtaca aaagcaatat tggcgctggg ttcatcttcc agctcttccg acttgctttt
    20461 cgcctccggt gaatcaatcg ctttctgttg cggaaaaagc atccgaaaac cgataaacgc
    20521 gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac
    20581 cagttgcccg gcgtaatacg ccaccatcat gatggcaaag acgtacaccg aggccatcaa
    20641 cgactgacga ttacgttcgg cactgttcat gttgcctgcc aggccaagaa ataacgcgac
    20701 agttgttaat gggttagcta acggtagtaa caccaccagc cccaggccaa ttgctttaaa
    20761 taaatctaac attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg
    20821 ggtaaacaac gataaagtgt cactctttag ctagccttgc atcgcattga ccaaaacttg
    20881 aaccgattta gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctggg
    20941 caatattatc ccctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga
    21001 tctgttcaat gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga
    21061 tcgcctgctt aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct
    21121 ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa ctgaaaagag tgttatcggt
    21181 cgacctgggg gcgctgaccc gtatgctgga tcgcctggtc tgtaaaggct gggtggaaag
    21241 gttgccgaac ccgaatgaca agcgcggcgt actggtaaaa cttaccacca gcggcgcggc
    21301 aatatgtgaa caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa
    21361 cctgacggcg gacgaagtgg caacacttga gcatttgctt aagaaagtcc tgccgtaaac
    21421 aaaaaaagag gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt
    21481 ttggactgga tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt
    21541 tcgggttact ctaaatggca cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta
    21601 ggccaataca tccgcagccg taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac
    21661 gagccgatac tctatctggc agaacgatat ggcttcgagt cgcaacaaac tctgacccga
    21721 accttcaaaa attactttga tgttccgcca cataaatacc ggatgaccaa tatgcagggc
    21781 gaatcgcgct ttttacatcc attaaatcat tacaacagct agttgataac gtgacaacgt
    21841 cactgaggca atcatgaaac cacttttatc cgcaatagca gctgcgttta ttctcttttc
    21901 cacgcagggc attgcggaac aaaccacgca gccggttgtt acttcctgtg ccaatgtcgt
    21961 ggttgttccc ccatcgcagg aacaaccacc gtttgattta aatcacatgg gtactggcag
    22021 tgataagtcg gatgcgctcg gcgtgcccta ttataatcaa cacgctatgt agttattgtc
    22081 aggctctgat cacagagcct gatatctccg taatatcttc ctgaagtctc aaatattttc
    22141 gccattgttg gcgatgagat cccgatacca gtaaaacgac gctttacgta tccgttccag
    22201 cgttcccttc ccttcgttgt ctttatcgac gtaaatcatt ccgtagcgct ttttcatttc
    22261 ccctgttccg gcagaaacca ggtcaatgca gccccacggt gtgtagccaa ttaagtcaac
    22321 accatcttca acaacggctt ttttcatttc ccgaatatgg gaagcaaagt aatcaatgcg
    22381 atagtgatcg ttcaccgtgc cgtcagcttg tctctggtca accgcaccaa atccattttc
    22441 gacaataaac agcggcaact ggaaatgatc ccagaaccag ttgagtgaat aacgtagccc
    22501 tgccggatca atttgccatc cccattcgga tgtatcgata taagggttgc tgaccaaatc
    22561 tcgtggctca acataatcca gttgcgggtt atctggcgaa aattgtgtcg taaaagacat
    22621 gtagtagcta aagccgataa agtcgacaca acctctggca agaatcgcat tatcttctgg
    22681 tgtgatatcg aggttgaatc ctttcctggc aaagtaattc agcatatgtt gcggataata
    22741 tccacgcgca tgaacatcag taaaccagta acgacgatgc atcgctttcg tggccatcat
    22801 catatcgttg ggtgcacacg tcagaggata gatggggcac atggcgatca tacagccgat
    22861 attaaattca ggattgatcg attttccagt ctgtaccgcc agggcactgg caactaactc
    22921 gtaatgtacc gcctggtaca ttatttgttc gcgctcattg atatcttcct ctggcgaata
    22981 caagataccg gaattagtaa atggacacag gctttcgctg aaattcacct gattattgat
    23041 ttcgttaaac gtcatccagt actttacttt ttctttatag cgcgtgaaga ccgttgatgc
    23101 gaagcggata aaaaagtcga tcagtttacg gtttcgccag ccaccatatt ttgtcaccag
    23161 atggtaaggc atctcaaaat gcgaaagcgt caccacaggt tccattccct gcttcaggca
    23221 ttcatcgaac agatcatcat aaaattttaa accctcttca ttcggctcct gttcgtcacc
    23281 ttgcggaaag attcgtgtcc aggcaatgga agttcgaaag catttgaatc ccatctcggc
    23341 aaataactga atatctgttt tatagcgatg ataaaaatca attgcttcat gattgggata
    23401 attaagtccg tcgataacgc cttctgtcac ttcacgcggc acaccgtgag cgccagcagt
    23461 catcacatca gcgatactga tgccttttcc tccttcattc cagccacctt ccaactgatg
    23521 cgcggctacc gcgccgcccc ataaaaaacc ttttttaaat cctgacatac acaactcctt
    23581 aactaaaata gattaccacc agacttccgc ctggatgccg gccataaact cgtctttgct
    23641 gttctcatta aacttaaaat tagataattc gttatccaga atattgatat acgttccata
    23701 gaagcgaatt tctggtcgtg aacctaaaat gctttcaccc actttcaatg catgccagag
    23761 tgtcgtttta taagccgatt catttagtgt tacaccctgc tgtgttttgt tctgttgctt
    23821 aaaccagcct aattcaagcc ccgtctgatt ccatgtattc cagatccagg ccggtcttac
    23881 tacggtgcga atactgtcaa aatcactatg agcgccactt tcataactat aaacatcttc
    23941 gccatgagaa tagacaagcg cgttagccat aataatattg tcagtaagat acatctcgcc
    24001 ctgagagatt aaacgccagg cgatcccatt ggtatggtca ccatagtaat agcgaccatg
    24061 cgccatcgtg ttactggcat ctgagaaact ggcaaaactg ctggcatagg aattattggc
    24121 aacctgtaac gtaaattcat taaacgtatc gcgttgcagg ttttgccgta aaacagaagt
    24181 cagcatccag ctatctttca tttcaaaata actgtcgtca ttttcattat cttgttgttg
    24241 atccgtttta tttggtgcag aatatttagc cattaatgat aatgttgcat catcccataa
    24301 cgggatattg cgataacgaa catccacaga attggtattc atctgactgg tacgcgtaaa
    24361 atcacgagag taaacatcga catcatcacg gcttaaggac atatcaaaca gtcctacacc
    24421 aagtgcccag ttttcaatcc ccacacccgc agcgacatcc gtggttaagg ttttccagtc
    24481 cagcatttgg atctcatatt gcgggagttt atgtttgcct acccagaagt ctgcctctgg
    24541 cgcgaagggt aaaaaacctc gcgttgtcag ataaatatca ctgaactgca tgatattttc
    24601 attggcactg tcaccaaacc aggcatcgtt atactgctca cccacgttgc catcataggt
    24661 aacgaccgca tttgccgttt taccgtcctg attataaaca cgctgattta aggtcaggtc
    24721 aaaccaaccg ctcatctcgt taccaaaacg tcccagagaa cccgctgcat aagtttgtgg
    24781 tgagccgtga ttaccagtac cccagcctga gcggaagtac ccctgatagc tgaacccaat
    24841 atcatccttt atatatttac tgatatcttt cagggtcacg ctttcgatag tggttttgtc
    24901 gcctttactt tcaataacgg caacttgctg cgatccattt atagtatgcg ctgcggcaga
    24961 cgagttctgt tcaccgtttt catttttgat gttatcagcg acgggagatg tcgctgatat
    25021 ttcagcaagt gaggctgatt tgtatttatt ttccgtaata cttttttgta aggtcgaaag
    25081 tcgggattta tataccccca gttcattctg tgttgctctc agctcttgtt tattttgcga
    25141 caattcattt tcaagcagtt caaggcgctg ctcaatggtt aattttgttg ccaacgcgtt
    25201 caacggaaac aatgctgtaa taatagaaaa agacaaaagg ctgacgttaa tcgtctttat
    25261 attcatgact tatccttaag agtgtaataa tttcctccag ctatcgcact ggaggaaaaa
    25321 atcaggcatg gagctctttt cttaatgaaa taatttccct ggctaaatcc tggcataata
    25381 ttgcattcat caaatgatcc tgtgcatgaa ccagaattaa attaacttct attttgccac
    25441 aaccttcatc ctgactaatt aacgcggtct gaatcttatg tgcctgaaga caggctaatt
    25501 ccgaactggc cattagctga gttgcgccct gccagtcttt ttgcccggca caacgtatgg
    25561 cttccatcgc atcagaacgt gcctgtcccg cgttgatcag taactccata acaagttctt
    25621 catctgcaaa catcaacatc tccttcctgt gtaaatggga ttatccgtta gttgttaagt
    25681 tattgacctt gttggcggca ataacaaaag gtaaatagat cattgtcgcc acacctaaac
    25741 ataaaaaacc caccagtaac gccagccagt ttcccccggt tccgaggaaa gcaattaaag
    25801 gtccgggcgt agtccagggc acggcataag caaccggtgg aataatttcc atcgaaacaa
    25861 agaagtaacc aatggcacag ttaacaatgg gaaccagaat aaacggcacc atcatgatgg
    25921 ggttaagtac aataggaagg ccgaacatta tcggttcatt gatattgaaa ataccgggga
    25981 taaacgacat ttttgccaga tcacggtatt ccgcacgctt agacgcgata aaaatagcca
    26041 acaataaccc caacgtcata cctgaaccgc cgcagttggc gaatgcatca ttaatagaag
    26101 tccaggtaat cggatatggc gcgccccagg tagtgccgtg ttgagcggcc caggagagat
    26161 tctccagatt agcctcagaa aaaacagttt ctcgaattgc cgaggtggta ttaggtccgt
    26221 ggatccccag cacccacagg aaattaccca cagcccccag gataattacc gcaaagatat
    26281 tcgttcccat atgtttgagc ggcgtctgga ttaatttgta aatgagatcg tttaatcctg
    26341 ccggggatat cagtgtcagg caataattaa gtgcggaaaa gaacaccatg acaaaaaata
    26401 ttggcaataa aactttaaat gaacgcgata ccgcaggagg tacagctgcc ggcatcgtta
    26461 tggtgatttt tgggtttcga gctaagcgac aaaatatttc actggtgatt aatgcaacga
    26521 taacagcaac aaacaacccc tgtgcgccga gatatttggt cgtcaggaat gcttgcccat
    26581 ctatcatctg atatggcgta taaacaataa aaaatgctcc tattgccgtt aatccgcata
    26641 aaagatcatc ttgctcatag ctaatcgcca tatttctggc gaccaggaaa gcaatcataa
    26701 ttgacatgat atttacagag ccattcatta ccggagtaaa aatagcttgt gctttttcaa
    26761 ggttggggaa aacgctgttc agatgcagca gcccggcaat aaagccgtcg ggcgataata
    26821 tggcaaagtt aattaatata attaatgagc ttgccatgat aattggaaac gtaaaaacga
    26881 atccatctct caccgctgc
//
LOCUS       DARNVM010000053        26895 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.053, whole genome shotgun sequence.
ACCESSION   DARNVM010000053 DARNVM010000000
VERSION     DARNVM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.053"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..334
                     /gene="dgoR"
                     /locus_tag="SI621_002003"
     CDS             <1..334
                     /gene="dgoR"
                     /locus_tag="SI621_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HEI4064730.1"
                     /translation="SDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNPVLQ
                     QLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMIASS
                     TRRLKEIT"
     gene            331..1209
                     /gene="dgoK"
                     /locus_tag="SI621_002004"
     CDS             331..1209
                     /gene="dgoK"
                     /locus_tag="SI621_002004"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEI4064731.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFVAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            1193..1810
                     /gene="dgoA"
                     /locus_tag="SI621_002005"
     CDS             1193..1810
                     /gene="dgoA"
                     /locus_tag="SI621_002005"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEI4064732.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            1807..2955
                     /gene="dgoD"
                     /locus_tag="SI621_002006"
     CDS             1807..2955
                     /gene="dgoD"
                     /locus_tag="SI621_002006"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEI4064733.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            3030..4367
                     /locus_tag="SI621_002007"
     CDS             3030..4367
                     /locus_tag="SI621_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064734.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(4364..5428)
                     /gene="cbrA"
                     /locus_tag="SI621_002008"
     CDS             complement(4364..5428)
                     /gene="cbrA"
                     /locus_tag="SI621_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HEI4064735.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQNGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMNAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            5529..6743
                     /locus_tag="SI621_002009"
     CDS             5529..6743
                     /locus_tag="SI621_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HEI4064736.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(6745..7077)
                     /locus_tag="SI621_002010"
     CDS             complement(6745..7077)
                     /locus_tag="SI621_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HEI4064737.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    7317..7389
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7383..7796
                     /gene="ibpA"
                     /locus_tag="SI621_002011"
     CDS             7383..7796
                     /gene="ibpA"
                     /locus_tag="SI621_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HEI4064738.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7908..8336
                     /gene="ibpB"
                     /locus_tag="SI621_002012"
     CDS             7908..8336
                     /gene="ibpB"
                     /locus_tag="SI621_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HEI4064739.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            8532..10193
                     /locus_tag="SI621_002013"
     CDS             8532..10193
                     /locus_tag="SI621_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HEI4064740.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(10190..10906)
                     /locus_tag="SI621_002014"
     CDS             complement(10190..10906)
                     /locus_tag="SI621_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4064741.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNANEYQVEYHLRRLHPEKS"
     gene            11203..12819
                     /locus_tag="SI621_002015"
     CDS             11203..12819
                     /locus_tag="SI621_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEI4064742.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYITAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            12819..14141
                     /locus_tag="SI621_002016"
     CDS             12819..14141
                     /locus_tag="SI621_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HEI4064743.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR"
     gene            complement(14138..15031)
                     /locus_tag="SI621_002017"
     CDS             complement(14138..15031)
                     /locus_tag="SI621_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEI4064744.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINKNVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKIILKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYSDGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLNARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKSFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            15198..16913
                     /locus_tag="SI621_002018"
     CDS             15198..16913
                     /locus_tag="SI621_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEI4064745.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGMAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFNDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16910..18403
                     /locus_tag="SI621_002019"
     CDS             16910..18403
                     /locus_tag="SI621_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEI4064746.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVTEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRRKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(18450..18899)
                     /locus_tag="SI621_002020"
     CDS             complement(18450..18899)
                     /locus_tag="SI621_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064747.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIHGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            19008..19355
                     /locus_tag="SI621_002021"
     CDS             19008..19355
                     /locus_tag="SI621_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HEI4064748.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19345..19707
                     /locus_tag="SI621_002022"
     CDS             19345..19707
                     /locus_tag="SI621_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEI4064749.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            19704..20201
                     /locus_tag="SI621_002023"
     CDS             19704..20201
                     /locus_tag="SI621_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEI4064750.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(20209..21393)
                     /gene="emrD"
                     /locus_tag="SI621_002024"
     CDS             complement(20209..21393)
                     /gene="emrD"
                     /locus_tag="SI621_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HEI4064751.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(21673..21762)
                     /gene="tisB"
                     /locus_tag="SI621_002025"
     CDS             complement(21673..21762)
                     /gene="tisB"
                     /locus_tag="SI621_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HEI4064752.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            22327..22425
                     /gene="ivbL"
                     /locus_tag="SI621_002026"
     CDS             22327..22425
                     /gene="ivbL"
                     /locus_tag="SI621_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HEI4064753.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            22531..24219
                     /gene="ilvB"
                     /locus_tag="SI621_002027"
     CDS             22531..24219
                     /gene="ilvB"
                     /locus_tag="SI621_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEI4064754.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLNVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            24223..24513
                     /gene="ilvN"
                     /locus_tag="SI621_002028"
     CDS             24223..24513
                     /gene="ilvN"
                     /locus_tag="SI621_002028"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEI4064755.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            25589..26071
                     /locus_tag="SI621_002029"
     CDS             25589..26071
                     /locus_tag="SI621_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064756.1"
                     /translation="MVKQLCFDVNDCAEVRELLRQSNGNITNDKIDEIIACLASPQGI
                     TCFYYSSAQGYQSRLQHTSQDVRELLSKSLDGATSLTLHLKKSGNSFFGSGKYLNEMD
                     FTINGFSQKMYSMAVNNVGNEVKLQCSRYFTTGRVDEQNSKIDKVTFTFFGAATTADL
                     "
     gene            26347..>26895
                     /locus_tag="SI621_002030"
     CDS             26347..>26895
                     /locus_tag="SI621_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064757.1"
                     /translation="MVSVTSYTYPTETENDNSQFQSQTQSQGLSQSLRDSIEGNIAAF
                     DAMEQSGLLDNCRTLSKNASFREIFRSLLLYILDSLHIYRHDISDKLVSYFHVTPESA
                     NANYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELNGKSLEKKSRDRDFSGRNVKEF
                     IGSLSEHDVLEKYQLSKVTVAAN"
BASE COUNT         6540 a         6622 c         7044 g         6689 t
ORIGIN      
        1 cagcgatctg gcgcagattg aatcggcgct gaacgagatg attgccaaca atcaggaccg
       61 cgaagcgttt aacgaagcgg atattcgcta ccacgaggcg gtgctgcagt cggtgcataa
      121 cccggtgtta cagcaactta gcattgcgat cagttcgctg cagcgggcgg tttttgaacg
      181 aacctggatg ggcgatgagg ccaacatgcc gcaaacgctc caggaacata aggcgctgtt
      241 cgatgcgata cggcatcagg acggcgatgc ggcagagcag gcggcgctta ccatgatcgc
      301 cagctcgaca cgaaggttaa aggaaatcac atgacagctc gctacatcgc aattgactgg
      361 ggatcgacca atctgcgcgc ctggctttat cagggcgacc actgcctgga gagcaggcaa
      421 tcagaagcag gcgtcacgcg cctgaacgga aaatctccgg ctgcggtgtt agcagaagtg
      481 acgaccgact ggcgtgaaga gaatacgcca gtggtgatgg caggaatggt cggcagcaat
      541 gtcggctgga aagttgcacc gtatttatcc gttcctgccc gtttttcgtc tattggcgaa
      601 caattaacgt ctgttggcga caatatctgg attattcccg gattatgcgt ctctcatgac
      661 gataaccata atgtgatgcg cggcgaagaa acacaattga tcggcgcgcg cactttggct
      721 ccttcctctc tttatgttat gcccggaacc cattgcaaat gggtgcaggc cgatagccag
      781 caaatcaacg attttcgcac cgtaatgacc ggtgaattac atcatttact gttaaatcac
      841 tcattgattg gcgcaggttt gccgccgcag gaaaactctg ccgatgcctt cgtggctggc
      901 cttgagcgtg gccttaatgc gcccgccata ttgccgcagc tttttgaagt tcgcgcctcg
      961 catgtgctgg gaacacttcc ccgcgaacag gtcagtgaat ttctctctgg tttgttgatt
     1021 ggcgcagagg ttgccagtat gcgcgactat gtgacccatc aacacgccat cacccttgtc
     1081 gccggaacat cgctgaccgc gcgctaccag caagcctttc aggcgatggg ttgcgacgtg
     1141 gcggcggtgg cgggcgacac ggcatttcag gctggtataa ggagcatcgc tcatgcagtg
     1201 gcaaactaaa ctcccgctga tcgccatttt gcgcggcatt acgcccgacg aggcgctggc
     1261 gcatgttggc gcggtgattg acgccgggtt cgacgcggtt gaaatcccgc tgaattcccc
     1321 acaatgggag cagagcattc ccgccatcgt tgatgcgtat ggcgacaagg cgttgattgg
     1381 cgcaggtacg gtactgaaac ctgaacaggt cgatgtgctc gccaggatgg gctgccagct
     1441 catcgttacg cccaatatcc atagtgaagt gatccgccgt gcggtgggct acggtatgac
     1501 cgtttgcccc ggctgcgcga cggcgaccga agcctttacc gcgctcgacg cgggcgcgca
     1561 ggcgctgaaa atattcccgt catcggcttt tggtccgcaa tacatcaaag cgttaaaagc
     1621 ggtattacca ccggacatcg cagtctttgc cgttggcggc gtgacgccag aaaacctggc
     1681 gcagtggata gacgcaggtt gtgcaggagc aggcttaggc agcgatctct atcgcgccgg
     1741 gcaatccgta gaacgcaccg cgcagcaggc agcagcattt gttaaggcgt atcgagaggc
     1801 agtgcaatga aaatcaccaa aattaccacg tatcgtttac ctccccgctg gatgttcctg
     1861 aaaattgaaa ccgatgaagg cgtggtcggc tggggcgagc ccgtgatcga aggccgcgcc
     1921 cgtacagtgg aagcggcagt tcacgaactg agcgactatt tgattggtca ggatccatcg
     1981 cgcatcaatg acttatggca agtgatgtat cgcgccggat tttatcgtgg cggtccaatc
     2041 ctgatgagcg ccatcgccgg gatcgaccag gcgttatggg atatcaaagg caaagtgctg
     2101 aatgcgccgg tctggcaact gatgggcggc ctggttcgcg acaaaattaa agcctacagt
     2161 tgggtcggcg gcgatcgtcc ggcggatgtt atcgacggca taaaaaccct gcgcgaaatc
     2221 ggcttcgata ccttcaaact gaatggttgt gaagaactgg ggctaattga taactcccgc
     2281 gcggtagatg cggcggtcaa caccgtggca caaattcgtg aagcttttgg caatcagatt
     2341 gagtttggtc ttgatttcca cggtcgcgtc agcgcgccga tggcgaaagt gctgattaaa
     2401 gaactggagc cgtatcgccc gctgtttatt gaagagccgg tgctggcaga acaagccgaa
     2461 tactacccga aactggcggc acaaacgcat attccactgg cggcgggtga acgcatgttc
     2521 tcacgtttcg attttaaacg tgtgctggag gcgggtggta tttcgattct acaaccagat
     2581 ctctcccacg cgggcggtat taccgaatgc tacaaaattg ccggaatggc agaagcctat
     2641 gacgtgaccc ttgcgccgca ctgtccgctc ggaccgattg cactggcggc ttgtctgcat
     2701 atcgactttg tttcctataa cgccgtactt caggaacaaa gtatgggcat tcattacaac
     2761 aaaggcgcgg agttactcga ctttgtgaaa aacaaagaag acttcagcat ggttggcggc
     2821 ttctttaaac cgctaacgaa accgggctta ggcgtggaaa tcgacgaagc taaagttatt
     2881 gagttcagta aaaatgcccc ggactggcgt aatccgctct ggcgtcatga agataacagc
     2941 gtagcagagt ggtaattcct gccacgtaag cccctcatcg ggcactaaaa cagcaataca
     3001 aaaatataac cctctgtaaa ttacagggca tggtgagcgg cttcgctatg cccaaaatct
     3061 ggagaaagct cgctatggat attcccgtta atgcagcaaa gccggggcgt cggcgttatc
     3121 tgacgctggt gatgatcttt attacggtgg tcatttgtta tgttgaccgc gctaacctgg
     3181 ccgtggcttc cgcccatatt caggaagagt tcggtattac gaaagcggaa atgggctatg
     3241 tattttcggc cttcgcctgg ctttatacgc tatgtcagat ccccggcggt tggtttttag
     3301 atcgcgtagg ttctcgcgtg acttatttta ttgcgatatt tggctggtca gtggcgactt
     3361 tattccaggg ctttgccacg ggattaatgt cattaattgg tctgcgcgcg ataaccggta
     3421 ttttcgaagc gcccgctttc ccgaccaata accggatggt gaccagctgg ttcccggaac
     3481 atgaacgcgc ttccgccgtt ggtttttata cgtctggtca gtttgtcggt ctggcgtttt
     3541 tgactccgct gctgatctgg attcaggaga tgttgagctg gcactgggtg ttcattgtca
     3601 ctggtggtat cggcattatc tggtcgctga tttggtttaa ggtttatcag ccgccgcgcc
     3661 tgaccaaagg tatcagcaaa gctgaactgg attacattcg tgatggcggc ggtctggtgg
     3721 atggtgatgc gccggtgaaa aaagaggcac gtcagccgtt aacagccaaa gactggaaac
     3781 tggtgttcca tcgtaaactg atcggtgtct atcttgggca atttgcggtg gcttctacac
     3841 tgtggttttt cttaacctgg ttcccgaact atttaaccca ggaaaaagga atcacggcgc
     3901 tgaaagcagg ctttatgacc acggtgccat tcctcgcggc gtttgtcggc gtcctgctct
     3961 ctggctgggt cgcggatctg ctggtacgta agggcttttc actgggcttt gcgcgtaaaa
     4021 cgccgattat ctgcggcttg ctgatctcca cctgcattat gggcgctaac tacaccaacg
     4081 atccgatgat gattatgtgc ctgatggcgc tggcattctt cggtaacggt tttgcttcga
     4141 ttacctggtc gctggtctct tctctggcac cgatgcgcct gattggttta accggcggcg
     4201 tgtttaactt tgccggtggt ctgggcggca tcaccgttcc gctggtggtg gggtacctgg
     4261 cgcagggtta cggtttcgca cctgcactgg tttatatctc cgccgtcgcg ttgattggcg
     4321 cgctctctta catcctgctg gtgggcgatg tgaagcgcgt tggctaatct ttcaactgtg
     4381 gaatatgcgc cacaccactg cgcataatcc actttcttaa agccggtgcg gtcaggcatc
     4441 ggctttttac tatcttaccg aagagtttta aacgcagttt gcgggtagcg cgccagtagg
     4501 cggcgttgct cttctccggc tgcttcagta acaccgaacg cagaatctct gcgctatcca
     4561 gcgcatagct aatcccctcc agcgaactgg cgctgataaa tcccgccgct tcaccaatca
     4621 gaaaggcgtt gtccttaccg cagacaaaat cctgccagcg tgagggaaac aacacggtac
     4681 atttctcact tttcaccgct ttgccaaact ggaactgaaa ggcgttcatt ttctctttca
     4741 gcgtcgtgaa acgcgtctga ccgtctttca tgggataggc accgccaaag ataaaataac
     4801 cgtctttgct gatactccat gagtaacagt cagttatcgc attatcaaag atgcaggagt
     4861 agaacggcac cggatgcttc tctgcgaacc actgctggat agcgacatat ttacgaattt
     4921 gatggtccgg gtagagatgt cgccgcacca tcgagttggc accatcggca ccgaccagat
     4981 agcgggcggt aatatgctgc tcccagccgt ctgcacgaaa aatgacatgc catttatcat
     5041 cctcacgcca gatttttcga cacaggctgt cgtgataaac ctcaacgctg gcggggatca
     5101 gcgatttcat ccacaagtcg aaagcatggc gattaatatt gatatagctt cgctggtagt
     5161 tacgcgtgag cgatgcggcg acgtcgacgg ttttgacgct gaaaatctgc ggattggcga
     5221 ttacatcgac aggaagcgtc agtccatcgc gaataaaaga tcgctgcgcg tccggtgcca
     5281 gcagaccgcc acaaggtttg ctgaaacctt cagtaccatt ctggtgcttt ttatccagcg
     5341 cgatcacctg cattttgcct gctaactttc gtgccaacgc cgaacctgcc gggccgaggc
     5401 caataatcgc cacgtcgaaa tgttccatct tttcactcac attcatcacg atggcgaaag
     5461 actattgtct gaaagtgaag tccttgtgaa gtatgatggc gggacccgtt ttgtatcagg
     5521 accgcgccat gaaacagata acctttgctc cccgtaatca cctgctcacc aataccaata
     5581 cctggacgcc cgacagccag tggctggtat ttgacgtgcg tccttctggc gcgtcgttta
     5641 ccggcgagac cattgagcgt gtgaatatcc ataccggcga ggtcgaggtt atctatcgcg
     5701 cgtcacaggg cgcgtacgtc ggcgtggtga ccgttcatcc gaagtcagag aaatatgttt
     5761 tcatccacgg cccggaaaat cctgatgaaa catggtatta cgacttccat catcggcgcg
     5821 gagtgattgt tgaaggcggc aaggtgagta accttgatgc gatggatatt accgcgccgt
     5881 atacgccagg ggcgctgcgc ggcggcagcc atgtgcatgt ctttagcccg aacggagaaa
     5941 gggtgagctt tacctataac gaccatgtaa tgcatcaact cgattcggcg ctggatttgc
     6001 gaaacgtcgg tgttgcggca ccgtttggcc cggtcaacgt acaaaagcag catccgcgtg
     6061 aatacagcgg tagccactgg tgcgtgctgg tgagtaaaac cacgcccaca ccgcagcctg
     6121 gcagcgatga aatcaatcgt gcttatgaag aaggatgggt aggaaatcac gcgctggcgt
     6181 ttattggcga cacactttcg ccaaagggcg agaaagtgcc ggagctattt atcgttgagt
     6241 taccgcaaga tgaagctggc tggaaagcgg caggtgatgc gccgttaagt ggaacggaaa
     6301 caaccctgcc cgcgccaccg cgtggcgtcg tgcagcgacg tttaaccttt acccaccatc
     6361 gggcttatcc ggggttagtc aacgttccgc gccactgggt gcgctgtaat ccgcagggta
     6421 cgcaaatcgc gtttttaatg cgtgatgata acggcattgt gcaactgtgg cttatctcgc
     6481 cacagggcgg cgagccgcgc cagttaaccc ataacaaaac ggatattcag tctgcattta
     6541 actggcatcc gtcaggagaa tggttgggct ttgtgctgga taatcgaatt gcttgcgccc
     6601 atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca cgccaatcca ccttctgcgg
     6661 acgccgtggt cttctcgccg gatggtcagt ggctggcgtg gatggaaggc ggccagctgt
     6721 ggatcaccga aactgatcgc taaatcagtt atcaggcatt ctggcgggtg gaataaccgc
     6781 attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt gagcgcaccg agctgtcttt
     6841 gcggaacaca tcccacggta gtaaaagcgt gtccatcaca gcggtaaacg gcatatcgag
     6901 aatcgccagc gacttagtgc cccagttggt ttcatcgtcg ccaatcatgg tggcgctggc
     6961 gcgggtgccg ggatacgttc cttctttccc cccggtatgg gacatcacgc tggagcatcc
     7021 tccaagtaat gtcattccgc tgcatatcat gaacgctaac aacacatttc ttatcataat
     7081 aaattcatct gttgatcgtg ggtgttggcc tgatgagtta tagcgatccc ttgctgaaaa
     7141 taacatcatc attacgtcgc actgtggcgg ctatcgcact ttaacgtttc gtgctgcccc
     7201 ctcagtctat gcaatagacc ataaactgca aaaaaaagtc cgctgataag gcttgaaaag
     7261 ttcatttcca gacccatttt tacatcgtag ccgatgagga cgcgcctgat gggtgttctg
     7321 gctacctgac ctgtccattg tggaaggtct tacattctcg ctgatttcag gagctattga
     7381 ttatgcgtaa ctttgattta tccccgcttt accgttctgc tattggattt gaccgtttgt
     7441 ttaaccactt agaaaacaac cagagccaga gtaatggcgg ctaccctccg tataacgttg
     7501 aactggtaga cgaaaaccat taccgcattg ctatcgctgt ggctggtttt gctgagagcg
     7561 aactggaaat taccgcccag gataatcttc tggtggtgaa aggtgctcac gccgacgaac
     7621 aaaaagagcg cacctatctg taccagggca tcgctgaacg caactttgaa cgcaaattcc
     7681 agttagctga gaacattcat gttcgtggcg ctaacctggt gaatggtctg ctgtatatcg
     7741 atcttgaacg cgtgattccg gaagcgaaaa aaccgcgccg tatcgaaatc aactaatttc
     7801 ctaaggccgc ctggcgcggc ctgacatctc catgctcgcc gtcagggagc atatgcgaat
     7861 cttcggattt gcaggtactt actcgcttct tagaaggaga aatgactatg cgtaacttcg
     7921 atttatcccc actgatgcgt caatggatcg gttttgacaa actggccaac gcactgcaaa
     7981 acgccggtga aagccagagc ttcccgccgt acaacattga gaaaagcgac gataaccact
     8041 accgcattac ccttgcgctg gcaggttttc gtcaggaaga tttagagatt caactggaag
     8101 gtacgcgcct gagcgtaaaa ggcacgccgg agcagccaaa agaagagaaa aaatggctgc
     8161 atcaagggct tatgaatcag ccatttagcc tgagctttac gctggctgaa aatatggaag
     8221 tctctggtgc aaccttcgta aacggtttac tgcatattga tttaattcgt aatgagcctg
     8281 aacccatcgc agcgcagcgt atcgctatca gcgaacgtcc cgcgttaaat agctaactag
     8341 ctattctctt tgccccgcca ttctggtggg gctttttttg tctttcttct tcaacaatgc
     8401 gttaattttc gcctgtttaa gatatttcgg cacgttcttt cccgtgataa tgtgcgctat
     8461 aaggcaaatc tgcttcaccg attcagccat aatccgtagc aattaaaatg ttatctcaag
     8521 agaaatggac gatgagtgat atagcattaa cggtcagtat tctggctttg gtggcagtcg
     8581 tcggtttgtt tatcggcaac gtcaaatttc gcggcatagg attaggtatt ggcggcgtgc
     8641 tgtttggtgg gatcatcgtc ggccattttg tttctcaggc ggggatgaca ttaagtagcg
     8701 atatgctgca tgttattcag gaatttggcc tgatcctgtt cgtttatacc atcgggattc
     8761 aggtgggtcc gggcttcttt gcctcattgc gcgtctccgg attacgcctc aacctgtttg
     8821 ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat cctgcataaa ctgtttgata
     8881 ttccactgcc ggtagtactg gggattttct ccggtgcggt aaccaatacg ccagcactgg
     8941 gggcagggca gcagatcttg cgcgacctgg gtacaccaat ggaaatggtc gatcagatgg
     9001 ggatgagtta cgcgatggcg tatccattcg gcatttgcgg gattttgttc accatgtgga
     9061 tgttgcgggt tattttccgc gtcaatgtcg agacagaagc ccagcagcac gagtcttcac
     9121 gcaccaatgg cggcgcgctg atcaggacta tcaatattcg cgttgagaat cctaacctgc
     9181 atgatttagc cattaaagat gtaccgattc tcaacggcga caaaattatc tgctcgcgtc
     9241 tgaaacgcga agaaacccta aaagtacctt cgccagatac cattatccaa ctgggcgatt
     9301 tgctgcatct ggtggggcag ccagcggatt tacataatgc gcaactggtg attggtcagg
     9361 aggtcgatac ctcgctgtct acgaaaggca ctgatttgcg cgtcgagcgt gtggtggtca
     9421 ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca ctttaaagaa cgctatgacg
     9481 ttgttatctc gcgcctgaac cgtgccgggg tcgaactggt cgccagtggt gatatcagcc
     9541 tgcagttcgg cgatattctc aacctggtgg ggcgtccgtc cgcaattgat gccgttgcca
     9601 atgtgctggg gaatgcgcag caaaaactgc aacaggttca gatgttgccg gtgtttattg
     9661 gtatcgggct tggcgtattg ttaggttcta ttcccgtctt tgtgccggga ttcccggccg
     9721 cgttgaaact ggggctggcg ggcggtccgc tgattatggc gttgatcctc gggcgtatcg
     9781 gcagtatcgg taagctgtac tggtttatgc cgccaagcgc caacctcgcg ctgcgggagc
     9841 tggggatcgt actgttcctc tcggtagtgg ggctgaaatc tggtggggat tttgtaaata
     9901 ccctggtcaa tggcgaaggg ctaagctgga ttggttatgg ggccctgatc accgccgttc
     9961 cgctgattac tgttggtatt ctggcgcgga tgttagccaa aatgaattac ctgaccatgt
    10021 gcgggatgct ggctggctcc atgaccgatc ctccggcgct ggcgtttgct aataatcttc
    10081 atccaacgag cggtgcggcg gcgctctctt acgccactgt ctatccgtta gtgatgttcc
    10141 tgcgcattat caccccccaa ttactggcgg tgctcttctg gagtatcggt taactctttt
    10201 ccgggtggag tcgccgtaaa tggtactcca cctggtattc gttggcattt ctgaacatca
    10261 ctgaataatt caaaaactcc ccgctctcgc tatatgacaa cgaggtgatc cgcagcagtg
    10321 gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct atcggcgagc accggcgtca
    10381 ggctttcata attaccgcca atcagaatcc cgcactcttg ttcaatatat tcaaacttcg
    10441 acccttccag atgttgcagc gataggttgc ggaacagttt taccggcata tagctgtctt
    10501 ccagcatcag cggtttccct tccacaaaac gaacgcgacg ggagaagtag atctgctcgt
    10561 tgatttgaat ccgtaactgg ctggcaatgg ccggaggcgc aggcatgatt tcaaaaatca
    10621 gcacctggct ggtgaccgtt tttccctggc gttttaacac ctccaccagc ccggtcagac
    10681 tggcggtttg atgcagtaca tctttgcgca ccaggtaagt gccgctaccg tggcggcgga
    10741 ccaccagccc ccacgctacc agcaggtcaa tggctttacg gatggtcatc cgcgataccg
    10801 caaattcttc cgccaacttt ttttcaccgg gaagaaggct gtttagcgtg aaatccgcgg
    10861 agttaagtcg aattcttaac cgctccgcaa tgcttttgta gatcatctct agatctcttt
    10921 tttgcctgca agcccgggta atgcgcccac aagttctgct taatcgattg aaaaaaaaat
    10981 ctttttttta aagatgtgtt cgatgctgtg taccagtgct cacagatgtc tactttttcg
    11041 cgaaaacgta gatctctacc gcccaacgaa aagcatgaaa gcgatcacga atcccattgg
    11101 gtcgtcatgt tctgccgatc gcatcttcct atcctcgctc caggcctgcc gcataaccaa
    11161 tcaggcttcc tacttacaga attgagaaaa gaggatgtgg aaatgctcag tcaaattcaa
    11221 cgctttggcg gcgcgatgtt cacgccagtg ctgctgtttc ccttcgccgg gattgtggtg
    11281 ggtcttgcca tcttgctgca aaacccgatg tttgtcgggg aatcactgac cgatccaaac
    11341 agtttgttcg cgcaaatcgt acacattatt gaagagggcg gttggacggt attccgtaat
    11401 atgccgctga tttttgctgt cggtttaccc attggccttg cgaagcaagc gcaggggcgt
    11461 gcttgtctgg cggtgatggt gagtttcctg acctggaact atttcatcaa cgcgatggga
    11521 atgacctggg gaagctactt cggcgtcgat ttcactcagg acgcggtggc aggtagcggg
    11581 ctgacaatga tggcggggat taaaactctc gataccagca ttatcggcgc gattatcatt
    11641 tccggcattg tgacggcgct gcataaccgt ctgttcgata aaaaactgcc ggtgtttctc
    11701 ggcattttcc aggggacatc ttatgtggtg attatcgcct tcctggtgat gatcccctgc
    11761 gcctggctga ctttgctcgg ctggccaaaa gtacaaatgg gaattgaatc tctgcaagcg
    11821 ttcctgcgtt cggcgggtgc gcttggggtg tgggtttaca ccttcctcga acgtattctg
    11881 atcccaaccg gtttacacca cttcatctac ggaccgttta tctttggtcc ggcagctgtt
    11941 gaaggcggca ttcagatgta ctgggcgcag catctgcaag agttcagttt gagcgccgag
    12001 ccgctgaaat cgttgttccc ggaaggcggt tttgccctgc acggtaactc aaaaatcttt
    12061 ggtgccgtgg gcatttcttt agcgatgtac atcactgccg caccggaaaa tcgggtaaaa
    12121 gtggcgggct tgctgatccc tgcaacctta accgccatgc tggtgggaat taccgaaccg
    12181 ctggaattta ccttcctgtt catttcaccg ttgctgtttg cagtacacgc cgtgctggcg
    12241 gcctcaatgt cgaccgtaat gtatctcttt ggtgtggtgg gcaacatggg cggtggtttg
    12301 attgaccagg ttttaccgca aaactggatc ccgatgttca gcaaccacgc ggatatgatg
    12361 ctgacccaaa tcgccattgg gttgtgcttt accctgctgt acttcgtggt tttccgcacc
    12421 ctgattctgc aattcaacat gtgcacgccg ggacgtgaag atgcggaagt gaaactctac
    12481 tcaaaagccg aatataaagc ctcgcgaggc caaaccaccg cggcagagcc aaaaaaagag
    12541 ctggatcagg ctgccggtat cctgcaagcc ctgggcgggg tcggcaatat ctccagcatc
    12601 aacaattgcg cgacgcgttt acgtattgca ctgcatgaca tgtcacaaac gctggatgac
    12661 gaagtcttta aaaagctggg agcgcacggc gtcttccgta gtggcgatgc cattcaggtg
    12721 atcattggtc tgcatgtatc ccagctgcgt gaacagctcg atagcttaat taattctcat
    12781 caatcagcag aaaatgttgc cattacggag gcagtataat gaccaaattc tcagtggttg
    12841 tcgcaggcgg tggaagcacc tttacgccag gcatcgtgtt gatgctcctg gcgaatcagg
    12901 accgtttccc gcttcgtgca ctgaaatttt atgataacga tggcgcgcgg caggaagtca
    12961 ttgctgaagc ctgtaaagtc atccttaaag agaaagcgcc ggatattgcg tttagttaca
    13021 ccaccgatcc tgaagtggca ttcagcgacg ttgattttgt catggcgcac atccgcgtcg
    13081 gcaaataccc gatgcgcgaa ctggatgaaa aaatcccgct gcgccacggc gttgttggac
    13141 aggaaacttg cggacccggc ggaatagcgt acggcatgcg ttccattggc ggcgtcctgg
    13201 aactggtgga ttatatggaa aaatattcac caaatgcctg gatgctcaac tactccaacc
    13261 cggcagccat tgtcgcagaa gccacgcgtc gtctgcgccc gaatgcgaaa atcctcaaca
    13321 tctgtgacat gccaatcggt attgaaagcc ggatggcgca aattgttggg ctgcaagatc
    13381 gcaaacagat gcgcgtgcgc tactacggcc tgaaccactt tggctggtgg acatcaattg
    13441 aagatttgca gggcaacgac ctgatgcccc agctgcggca atatgtctct aagcacggtt
    13501 atgttccacc gcagcaagat acacatactg aagcgagctg gaacgacacc tatgcaaaag
    13561 cgcgggatgt ccaggcactg gacccggata cattgccgaa cacctatctg aaatattatc
    13621 tcttcccgga ttatgtcgtt cagcattcca accctgaaca tacccgcgcg aatgaggtga
    13681 tggagcatcg cgagaaacag gttttcgatg cttgccgcgc cattacggcg gcaggaaatt
    13741 cagcggcggg caagctggaa attgacgaac atgcgtcata catcgtcgat ctggcggcgg
    13801 caattgcctt caacactcag gagcggatgt tgctgattgt gcctaacaac ggggcaatcc
    13861 ataactttga tgatgaagcg atggtcgaga tcccgtgtct ggtcgggcac aacggaccag
    13921 aaccactggt ggtcggcgat atcccgcagt ttcagaaagg gttaatgagt cagcaagtgg
    13981 cggtggaaaa actggtcgtg gacgcctggg aacagcgttc atatcagcac ctgtggcagg
    14041 cgattacgtt gtcgaaaacg gtaccgagcg ccgcggtcgc caaagctatt ctggatgagt
    14101 tgctggaggc caacaaagcg tactggccag agttacgtta attgtgtgga ctggcatccg
    14161 ttgggtgctg gtccgtattc ttcagcacag ctgacggcgc ataaccaaat ttatgtttaa
    14221 aggatttgct gaaatgaaag gagtcgaaga agttgagcgt atcagcgact tcgccaaccg
    14281 agttcccttg ttgctttaat agcgatagcg ccagatccag acgtgcattg aggtaatatt
    14341 ctttcggcgt cttgccggta tagcgtaaaa acagacgacg caaccaggct tcgctacagg
    14401 ggatcgtggc agccatatca gctacgctcc agcgttgttg cagactggca tgtaaagtgg
    14461 caattagttt ttcaatttgc cgccgttgtg gatctttttt accgtctgag tacataagac
    14521 aaatccactg ataaataatt ttcgtcagaa aagcgactgc cagattattt tttattgctt
    14581 ctggtgaagt aattaactca gcaacttcgg tgagttcctg attataaatt tcgccattat
    14641 aaataacgct ttgctgaccg acgggaatat ccatcatact ggtgggggta aattccatcc
    14701 agtactgttc ccagactaaa ccttcacagt gataagagtg aatatccatt ggctttaaaa
    14761 atataataca gttgccatgc agtgttattt gttcaccatt ctttaaaata attttcccac
    14821 atccttgtac ggtatacacc gcaacccatg agttggcgat aagcggtttc ttcttgtccc
    14881 gcggccagat cacccgataa tgctcgtcgg cgtaggtatg ccagagcgag atcagagaaa
    14941 ttccgctgga gatcacattt ttgttaatca ataagggaat attgtacgga gtttcgtttt
    15001 ttacatccga actttgcaat tttccattca ttttaatatc cctccggcga attatgctca
    15061 caacctgaac ttaacagaaa acaatccgtt cagggatacg atgtttgtat tttaagaatc
    15121 tgactgcctg acccgacgca ttttacctct ccctatattc atgcgtccgg gacataaaca
    15181 taaataaggg ctatgagatg aattcgttac aaatcttgag ttttgtcggt tttacgctgc
    15241 tggtggcggt catcacctgg tggaaggtcc gcaaaacaga taccggatcg caacaaggct
    15301 attttcttgc cggacgttca ctaaaagcgc cggttattgc cgcttcgtta atgttaacca
    15361 acctttccac ggaacaactg gttggacttt ccgggcaggc ctacaaaagc ggcatgtcgg
    15421 tgatgggctg ggaagtcact tctgcggtga cgctgatctt cctcgcgcta atctttttac
    15481 cgcgctatct gaagcgtggc attgccacca tccccgattt cctggaggaa cgttatgata
    15541 aaacgacgcg tattatcatc gacttctgct tcctcattgc caccggcgtc tgctttctgc
    15601 ctattgttct ctactccggc gcgttggcgc tcaacagcct gtttcacgtc ggggaatcgt
    15661 tacaaatttc ccacggcgca gcgatctggc tactggttat tttgcttggt atggcgggaa
    15721 ttttgtatgc ggtgatcggc ggactgcgcg caatggcagt ggcagactcc attaacggta
    15781 ttgggctggt tattggcggg ttgatggtgc cgatatttgg cctgatcgcg atgggcaagg
    15841 gcagctttat gcagggcatt gagcaactca ccaccgttca cgccgagaaa ttaaactcag
    15901 tcggcggccc gaccgatccc ttgccgattg gcgcggcatt taccggtttg attctggtga
    15961 acacctttta ctggtgtaca aatcagggca tcgtgcaacg cacactggcg tcaaaaagcc
    16021 tggcggaagg gcaaaagggg gcgctgttaa cggcggtgct gaaaatgctc gacccgctgg
    16081 tactggtgct gccagggttg attgcgtttc atctgtatca ggatttacct aaagccgaca
    16141 tggcctaccc gacgctggtc aataacgttc tgccagtgcc actggtgggt ttcttcggcg
    16201 cggtgttatt tggtgcggtg atcagtacct tcaacggctt tctgaatagc gccagtacgt
    16261 tattcagtat gggtatttac cggcgcatca ttaaccagaa tgccgagccg cagcagctgg
    16321 tcaccgtcgg gcgcaaattt ggtttcttta tcgctatcgt ttcggtgctg gtcgcgccgt
    16381 ggatcgccaa cgcgccgcag gggctatata gctggatgaa acagctcaac ggtatttaca
    16441 acgtgccgct ggttaccatc atcattatgg gctttttctt cccgcgcatt ccggcgctgg
    16501 cggcaaaagt ggcgatgggg attggcataa tcagctacat caccatcaac tatctggtga
    16561 agttcgactt ccatttcctc tatgtgctgg cctgtacgtt ctgcatcaac gtggtcgtga
    16621 tgctggtgat cggttttatc aaaccgcgcg ccacgccgtt caccttcaac gatgcgtttg
    16681 cggtggacat gaaaccgtgg aaaaacgtca agatcgcgtc aattggcatc ctgttcgcga
    16741 tgattggcgt ctatgccggg ctggctgaat tcggcggcta cggtacgcgc tggttagcga
    16801 tgatcagtta tttcatcgct gccgtagtga ttgtctacct gatttttgat agctggcggc
    16861 atcgtcacga cccagccgta acctttactc ccgacgcgaa ggatagccta tgaaacgccc
    16921 caattttctg ttcatcatga ccgataccca ggccaccaat atggtcggtt gctatagcgg
    16981 taagccgctg aatacgcaaa atattgatag tctggcggcg gaaagtattc gctttaattc
    17041 cgcctacacc tgttcaccgg tttgtacacc agcgcgcgcc gggctgttta ccggtatcta
    17101 cgctaaccag tccggcccgt ggaccaacaa cgtcgcgccg ggcaaaaaca tctccactat
    17161 gggacgctac tttaaggatg ccggctatca cacctgttac atcggcaaat ggcatctcga
    17221 cggtcatgac tatttcggca ctggcgagtg tccgccggag tgggacgctg attactggtt
    17281 cgatggggcg aactacctta gcgaactgac ggaaaaagaa attagcctgt ggcgcaatgg
    17341 cctaaacagc gtcgaagatt tacaggcgaa ccatatcgac gaaaccttca cctgggcgca
    17401 ccgcatcagc aatcgggcgg tagattttct gcaacagccc gcgcgcgccg aggaaccctt
    17461 cctgatggtg gtttcgtatg atgagccgca tcacccgttc acctgtccgg tggagtattt
    17521 agagaaatac gctgattttt actacgaact tggcgagaaa tcacaggatg acctggcgaa
    17581 caaaccggaa catcaccgct tatgggcgca ggcgatgcca tcgccagtag gtgatgacgg
    17641 gctttatcac catccgctct attttgcctg caatgacttt gttgatgacc aaatcggacg
    17701 ggtcatcaat gccttaacgc cagagcaacg tgaaaatacg tgggtcattt atacctccga
    17761 tcatggtgaa atgatgggcg cacataagct gatcagtaaa ggagcggcga tgtatgacga
    17821 catcacccgt attccgctga tcatccgttc gccgcaaggg gagcggcgac aggtcgatac
    17881 gccagtcagt catatcgatt tactgccgac aatgatggcg ctggcagata ttgaaaaacc
    17941 agagattctg ccgggggaaa atatccttgc cgtgacagag ccacgcggcg tgatggtgga
    18001 atttaaccgc tacgagattg agcatgacag ctttggcggt tttattccgg tgcgttgttg
    18061 ggtgacggat gactttaaac tggtactcaa cctcttcacc agtgatgaac tttacgatcg
    18121 ccgtaatgac ccaaatgaaa tgcataacct gatcgatgat atccgttttg ccgacgttcg
    18181 ccgcaaaatg catgacgcct tattggatta catggacaaa attcgcgatc cgttccgcag
    18241 ttatcaatgg agcctgcgtc cgtggcgtaa agatgcacga ccgcgctgga tgggggcgtt
    18301 tcgtccacgc ccacaagatg gctattcgcc agtggtacgc gactatgaca ccggcctacc
    18361 gacacaaggg gtgaaggtgg aagagaaaaa acagaagttc tgacgataat gcccgctgat
    18421 gaacacccgg cgggcattat tcaggctgct taaaccagtg aaccgccggt caggaaggaa
    18481 aagataagga atatcagcaa aatgctgcac acgatgccga taccaaaaag caaggtatgg
    18541 aaagccagcg tcccgccgcc aaaaaccagc gcgccgtgaa tgccccagcg attttcctta
    18601 cgcataccgc caacaaatgc gcaaagtagc gcagcaatgg ccagtgcgat accgctttga
    18661 tcgaggattt tatcgacatc cacgtcctct tttttctcca cggtggtaat tttttcgcct
    18721 tttattccgg cgagaagacc tcttttaatt tcagccgttt tatccgccac gatgctttca
    18781 agcgttggcg gtggtgcgga aaaagggccg aatgaaaagt ggataatccc catcattaaa
    18841 gcgatggcac cgaacagcat gcccaatgaa ctgattttat tttgcgctat gatacccatc
    18901 tgacctcctt gtagcggatg gcgaaagcat atcaggaaac gggcggacga aattcttaaa
    18961 aaaaaccgct aaaggtagag cgttagtgaa attgcaaagg agcaacaatg aaaatttccc
    19021 gcctcggaga agcacctgat tatcgcttct cgctggctaa tgagcgtact tttctggcgt
    19081 ggatccgtac cgcactaggc tttctggcgg cgggggttgg gcttgatcaa cttgcgccag
    19141 atttcgccac gcccgtcatt cgcgaactgc tggcgttgtt gttgtgtctg ttttccggcg
    19201 ggttagcgat gtatggctac ctgcgctggt tacgcaatga aaaagcgatg cgtctgaaag
    19261 aggacctgcc ctacaccaac agcttattaa tcatcagctt aatattgatg gtcgtagcgg
    19321 tgatcgttat gggactggtg ttgtatgccg gatagccgca aagccagacg cattgccgac
    19381 ccagggctgc agccggagcg tacatcactg gcgtggtttc gtaccatgct gggctacggc
    19441 gcgttgatgg cgttggctat caaacacaac tggcaccagg cgggcatgtt attctggatt
    19501 tcgattggca ttctcgccat tgtggcgctg atcctctggc actacacccg taatcgcaat
    19561 ttaatggatg tcacgaatag cgatttttcc caatttcacg tagtccgtga caaatttttg
    19621 atctccctcg cggtgttatc tctcgcaata ctgtttgctg taacgcatat acatcaactt
    19681 atcgtattta ttgagagagt cgcatgacag gaagtcaggt cattgatgct gaagaggaca
    19741 ggcataaact ggttgtagag tacaaggatg ctctgcaacc tgccgatttt tatcacaatt
    19801 tcaaacaacg gggcatccgc tccgtacagc tcattcccca tcttgaattt gatgaccggg
    19861 gcgatctgac ggccgcttcg gtgacagcag agctgtgggg gaaattttta attgccctgt
    19921 tcgagtgttg ggtacgggca gatatcagtc gtatctcgat tgagcttttt gacgccaccc
    19981 tacaaaaatg gtgtggcagc gaaaacccgc aaccgcgccg tggctgccag gcgtgcgact
    20041 ggcatcggct gtgcccgcac gctcgggagg agacgccaga cagcgtgcta tgtgcgggct
    20101 atcaggcgtt ttattcttac tccgcaccgc atatgcgggt gatgcgtgat ctgataaagc
    20161 agcatcgttc tcccatggag ctgatgacga tgctgcggtg acgtgcgctt aaacgggctg
    20221 cccctgatgc gacatccgcg tcgccagcgg tagccagcac agcacgatca acaatcccat
    20281 taaggtcatc aacaacccca ggctgccctg accggtttgc ggcaacatcg cagagagcga
    20341 cgcaagcacg ccggaaccaa tgttttgcag accgccgacc agcgcgcccg cggtgcccgc
    20401 caggaagggg aacggttcca tcgcgccgct ggtcgccagc ggaaacagca tcccggcacc
    20461 gaaaaagaac agcgcggcgg gaacgagcag cgtccagaca ttcatcacgc caaaccagtc
    20521 ggggatccac atcagcaagc cagccagcag gcagcagata accgactgcc acatcaacgt
    20581 tgagaagcgt ttattgggac gtccggcaaa ccatgcccca aaaaatgccg ccggaatcgg
    20641 cagaataaac aaaatactga ccgtcatact gctcagccct aataccgcgc ccatcagcac
    20701 gccggagcag gcttcaaagg cggcaatccc ggccagaccg ccaatcagca tcagcaaata
    20761 acagttaaaa ccgctgttac cgaaaagcgt tttataactg gtaagcaggc gcgtgcgcgg
    20821 tgcgtcgacc ggacgcgttt ccggcatcca gcgggccata ctgaaggtca caccggcaca
    20881 aagtaccaac aaaaagagat aacaggcgcg ccagttccac atcgtatcca gcagaccgcc
    20941 gattagcggt gcgagcaacg gactgacaag aattcccatg tttaacaagc tgttagcgtg
    21001 gcgcaactgt gtccgttcat ataaatcacg cggcaaagta cgcgccatta cgccgccaac
    21061 gccggtgccc atcccctgca tcgcgctggc ggcaatcaat actgtcaaac tggaggtcgt
    21121 gaccgcgacc agcgttgcca gcataaaaat ggacattccg acgaggatca ccggtcggcg
    21181 acccacacgg tcggaaattg ggccataaaa cagctgtgag acaccgtaag tcagcagata
    21241 agcgcccatt acgctctgca ccgccccttc acggacgttg agatcgcgcg ccatatcggc
    21301 aatagctgga atataaatgg tttgcgccat ctgaccgacg gccacgagta ataccaacat
    21361 caataacaaa ttgacgtttc tatgcctttt cattatcacg gatgctttta taaaaataat
    21421 tagaataagt cactacgcat tcccataaat gcgggagagg aatctatcac attgataaat
    21481 gttagccaga tatacgccag gaatggggaa ttgtttagcg gcagaatatg taaacaaaag
    21541 cggcaataaa tgttgccggg agggatggta gggcggataa gatgcatcag catcgcatcc
    21601 gacaccaacc cgcacgctaa atatgaaagg ggagcggtta cccgctcccc tttggtgcga
    21661 cttgaatctg aattacttca ggtatttcag aacagcatca agcagttgca gtgctgcaac
    21721 aatgagtttg aggataagaa tggcgatatc caccaggctc atacgcgtct cctgtggttc
    21781 aggagcactg ccagctgacg tacctttccg ctgcctgttg ccagctgttg tgcacgcggt
    21841 atctcaagga gaaacgttgt gctcgttgac ataacacagt gtgctcacgc ggggttaagg
    21901 cgctgacggc accacccgtt tcagccagga cttagtgcgc cgggtaacga tgcggccccc
    21961 gcataacaca ttgcgtacag tgatattata gaagtttact gtataaataa acagtaattt
    22021 ttggacaaaa cgcaaacagt gatcgatact caactatgca ctaattacgt caaaattcgt
    22081 gccgaaattg cgcgttctgc gcggaacacg tatactttct gtgttgacat aatacagtgt
    22141 gctttgcggt taccagccgc gggcggctga cgaaacctcg caccggcggg gttttttgtt
    22201 acctgcagta cagtacaaaa cgtgatcaac ccctcaattt tcccttgctg aaaaattttc
    22261 cattgtctcc cctgtaaagc tgtgcttgta taaatattgt taaacacaaa accaacaagg
    22321 tccccaatga ctacttccat gctcaacgca aaactactac caactgcgcc atccgccgca
    22381 gtggtcgtcg tgcgtgtggt ggtggtcgtc ggcaatgcgc cgtagggact ggaacaacac
    22441 acgattccaa aaccccgccg gcgcaaaccg ggcggggttt ttcgtttaag cacctcccgg
    22501 aaagtcggcc cagaagaaaa ggactggagc atggcaagtt cgggcacaac atcaacgcgt
    22561 aagcgcttta ccggcgcaga atttatcgtt catttcctgg aacagcaggg cattaagatt
    22621 gtgacgggca ttccgggcgg ttctatcctg cctgtttacg atgccttaag ccaaagcacg
    22681 caaatccgcc atattctggc tcgccatgaa cagggcgcgg ggtttatcgc tcagggaatg
    22741 gcgcgcaccg acggtaaacc ggcggtctgt atggcctgta gcggaccggg ggcgactaac
    22801 ctggtgaccg ccattgctga tgcgcggctg gactccatcc cgctgatttg catcactggt
    22861 caggttcccg cctcaatgat cggcaccgac gccttccagg aagtcgacac ctacggcatc
    22921 tctatcccca tcaccaaaca caactatctg gtcagacata tcgaagaact cccgcaggtc
    22981 atgagcgatg ccttccgtat tgcgcaatca ggccgccctg gcccggtgtg gatagacatt
    23041 cctaaggatg tgcaaacggc cgtttttgag attgaagcac agcccgcggt ggcagaaaaa
    23101 gccgtcgcgc ccgcctttag cgaagaaagc attcgtgacg cagcggcaat gattaatgct
    23161 gccaaacgcc cggtgcttta tctgggcggt ggtgtgatca atgcgcctgc gcgagtgcgt
    23221 gaactggcgg agaaagcgca actgcctacc accatgactt taatggcgct gggcatgttg
    23281 ccaaaagcgc atccgttgtc gctgggtatg ttggggatgc acggtgtgcg cagcaccaac
    23341 tatattttgc aggaggcgga tctgctgatt gttctcggcg cgcgttttga tgaccgggcg
    23401 attggcaaaa ccgagcagtt ctgcccgaat gcgaaaatca ttcatgtcga tatcgaccgt
    23461 gcagagctgg gcaaaatcaa gcaaccgcac gtagcgattc aggcggatgt tgatgacgtg
    23521 ctggcgcaat tgatcccgct ggtggaagcg caaccgcgtg cagagtggca ccagttggtg
    23581 gcggatttgc agcgcgaatt cccgtgccca atcccgaaag cgtgcgatcc gttaacgcat
    23641 tacggcctga tcaacgccgt tgccgcctgt gtcgatgaca atgcgattat caccaccgac
    23701 gtgggtcagc atcagatgtg gaccgcgcaa gcctatccgc tcaatcgccc gcgccagtgg
    23761 ctgacctccg gtgggctggg cacgatgggc tttggtctgc ctgcggccat tggcgcggcg
    23821 ctggcgaacc cggatcgcaa agtgttgtgc ttctccggcg acggcagcct gatgatgaat
    23881 attcaggaga tggcgaccgc cagtgaaaat cagctgaatg tcaaaattat cctgatgaat
    23941 aacgaagcgc tgggactggt gcatcagcaa cagagtctgt tctacaagca gggcgttttt
    24001 gccgctacct atccgggaaa aatcaacttt atgcagattg ccgccggatt cggcctcgaa
    24061 acctgtgatt tgaataacga aaccgatccg caggctgcat tgcaggaaat catcaatcgc
    24121 cctggtccgg cgctgatcca tgtgcgtatt gatgccgaag aaaaagtgta cccgatggtg
    24181 ccgccaggtg cggcgaatac tgaaatggtg ggggaataag ccatgcaaaa cacaactcat
    24241 gacaacgtga ttctcgaact caccgttcgc aaccatccgg gcgtaatgac ccacgtttgt
    24301 ggcctttttg cccgccgcgc ttttaacgtt gaaggtattc tttgtctgcc gattcaggac
    24361 agcgacaaaa gccatatctg gctactggtc aatgacgacc agcgtctgga gcagatgata
    24421 agccaaatcg ataagctgga agatgtggtg aaagtgcagc gtaatcagtc cgatccgacg
    24481 atgtttaaca agattgcggt gttttttcag taaggagcga cattaaaccg ctcgggggag
    24541 ttactgatga gcgatttttg atagatccgt tcagatatat agggtgaaat tgtgccagag
    24601 accatctcag gcacaattta ttagcccagg acgttaattt aagtcgctgc accggtaacc
    24661 taggggtgac agttagggct aaattttcca gcgatggttt taaattctga ttgctgtcta
    24721 taccagtggt catgaccagc attctattaa ttaatggttc atcgtgaggt atattatggc
    24781 aagtcctata aacagcggaa taatgatggc aaatctctgc ccttccacct ttttggataa
    24841 aaatataaat atcgctgctg agcttgatat taaaaataac gagaaaaaat attcgcctgg
    24901 cagtaatttt gcaaaatgga tgttgcagga aataaagcac ttaatactaa atatcatgtc
    24961 cggttctcgg agtgttaaca ccgatattct tgactatttc caccctatgc caggcacgga
    25021 aaataatggc aatcgtacct ggatggcggc aactggagag aacgaatata ccgaaatcaa
    25081 acaaacaggt gataaagcct ttaaagttat acagtatgac gccggtgaaa aaaatacaaa
    25141 aaaaacagta aaagaaacat catattctgg agtggctgtg gcgactatta tcaagtcgct
    25201 gtctgaaaaa actgctgcgt tagaaacaca ttctgctgac atggttttga ggaaaaaatt
    25261 agttaatagt attgttctga tgaatgcgga ttgtaattac gaaatgcctg cagaaatttt
    25321 gagtaataca tatgatttat taaacctgcg aattcaaaaa gatgaggggc atttacctgt
    25381 tcaggaaaaa attgatattg atgatgatta tttacactct atgactatgg atgctcatag
    25441 atgtataaaa gaacaaacta aaccaagtgg cgctgcaaaa ataacttgct gtggtgttga
    25501 gttaagtaaa gatattctta aaaagtttgc aaacaaaatt gaaaaaattg aacaagatcc
    25561 aaaatatatt caaaacacac ttgaagaaat ggttaaacag ctttgctttg atgttaatga
    25621 ttgtgctgag gtaagagaat tgttacggca atcaaacggt aatattacga atgataagat
    25681 tgatgaaata atagcttgct tagccagtcc tcaggggatt acatgttttt attattcttc
    25741 cgcacaaggc tatcagagtc gcttacaaca cacatctcaa gatgttcgcg agttactctc
    25801 aaagagtttg gatggtgcca cttcattaac attgcatttg aaaaaatcag gaaattcttt
    25861 ttttgggtct ggaaagtatc ttaatgaaat ggattttact ataaatgggt ttagtcagaa
    25921 gatgtattcg atggcggtta ataatgttgg aaatgaagtt aagcttcagt gttctcgtta
    25981 ttttactacc ggtagggtcg acgaacaaaa ctccaaaatc gataaagtca cttttacatt
    26041 tttcggtgcc gcaacaactg ctgatttatg atttttgcct gataaatcgc gtttatcatt
    26101 aaggtgaacg cgtattgtta accgtctgtg tataaacagt ctttttgaaa taatgagcga
    26161 tttttgatag tgaataagta gaattagtga tgcaattgca ttgggaaaat cgttaaaaat
    26221 taacgacaca tggtcttatt attgaaataa atgaaatata gcggttagca ttgacatatt
    26281 tatcatgcta accattgatg cttctaaaat gtcaaaatat gcaaagtgtc atattgcgag
    26341 gttactatgg ttagtgtgac gagttataca tatccaacgg aaacagagaa tgataatagc
    26401 cagtttcaga gtcaaactca gagtcagggc ctgagccaga gtctaagaga tagtatcgag
    26461 ggtaatattg ctgcatttga tgcgatggag caaagcggcc tgctcgataa ttgtagaaca
    26521 ttatctaaaa atgcctcttt tcgtgaaatt ttccgaagtt tattgttata catcttagat
    26581 tccttacata tctatcgaca tgatattagt gataaattgg tttcttattt tcatgttacg
    26641 cctgaatcag cgaatgcgaa ttataaaaca tggatcgcta ccacaggcat ggatagctat
    26701 ataaaaattg agcagaccgg cagtgataaa tttaaagtca ctgatgtgta tcgtgagtta
    26761 aatggcaaga gtttggaaaa aaagagtagg gatagggatt ttagtggtcg aaatgtgaaa
    26821 gagtttatag ggtcattatc agagcacgat gtattggaaa agtatcaact ctcaaaagta
    26881 acagttgctg caaat
//
LOCUS       DARNVM010000054        26654 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.054, whole genome shotgun sequence.
ACCESSION   DARNVM010000054 DARNVM010000000
VERSION     DARNVM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.054"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            1..435
                     /gene="cmtB"
                     /locus_tag="SI621_002031"
     CDS             1..435
                     /gene="cmtB"
                     /locus_tag="SI621_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HEI4064758.1"
                     /translation="MDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQSIK
                     DSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSA
                     ADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            463..1851
                     /gene="cmtA"
                     /locus_tag="SI621_002032"
     CDS             463..1851
                     /gene="cmtA"
                     /locus_tag="SI621_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEI4064759.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1866..3143
                     /locus_tag="SI621_002033"
     CDS             1866..3143
                     /locus_tag="SI621_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEI4064760.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QMLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3140..4105
                     /gene="yggF"
                     /locus_tag="SI621_002034"
     CDS             3140..4105
                     /gene="yggF"
                     /locus_tag="SI621_002034"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEI4064761.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGQVVIGEGEIDHAPMLWIGEDVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRGSLLHAPDMYMKKLVVNRLAAGAIDLSMPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4127..4636
                     /gene="fumE"
                     /locus_tag="SI621_002035"
     CDS             4127..4636
                     /gene="fumE"
                     /locus_tag="SI621_002035"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HEI4064762.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMAWDFISNVNSIIRNASLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4633..5346
                     /locus_tag="SI621_002036"
     CDS             4633..5346
                     /locus_tag="SI621_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEI4064763.1"
                     /translation="MKIELTVNGLRVQAQYSDDEIENVHKPLLRMLAALQAVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHKLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALDVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5318..5995)
                     /locus_tag="SI621_002037"
     CDS             complement(5318..5995)
                     /locus_tag="SI621_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEI4064764.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5989..6666)
                     /locus_tag="SI621_002038"
     CDS             complement(5989..6666)
                     /locus_tag="SI621_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEI4064765.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6654..7361)
                     /locus_tag="SI621_002039"
     CDS             complement(6654..7361)
                     /locus_tag="SI621_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEI4064766.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7362..7940)
                     /locus_tag="SI621_002040"
     CDS             complement(7362..7940)
                     /locus_tag="SI621_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEI4064767.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7963..8394)
                     /locus_tag="SI621_002041"
     CDS             complement(7963..8394)
                     /locus_tag="SI621_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEI4064768.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTTLLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8766..9785
                     /gene="epd"
                     /locus_tag="SI621_002042"
     CDS             8766..9785
                     /gene="epd"
                     /locus_tag="SI621_002042"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEI4064769.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFSWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9835..10998
                     /gene="pgk"
                     /locus_tag="SI621_002043"
     CDS             9835..10998
                     /gene="pgk"
                     /locus_tag="SI621_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEI4064770.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11213..12292
                     /gene="fbaA"
                     /locus_tag="SI621_002044"
     CDS             11213..12292
                     /gene="fbaA"
                     /locus_tag="SI621_002044"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEI4064771.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12483..13343
                     /gene="mscS"
                     /locus_tag="SI621_002045"
     CDS             12483..13343
                     /gene="mscS"
                     /locus_tag="SI621_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HEI4064772.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13482..14117
                     /gene="argO"
                     /locus_tag="SI621_002046"
     CDS             13482..14117
                     /gene="argO"
                     /locus_tag="SI621_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HEI4064773.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQHHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14210..14950
                     /locus_tag="SI621_002047"
     CDS             14210..14950
                     /locus_tag="SI621_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HEI4064774.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15118..16014
                     /locus_tag="SI621_002048"
                     /pseudo
     CDS             15118..16014
                     /locus_tag="SI621_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16011..17106)
                     /locus_tag="SI621_002049"
                     /pseudo
     CDS             complement(16011..17106)
                     /locus_tag="SI621_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(17309..18202)
                     /gene="argP"
                     /locus_tag="SI621_002050"
     CDS             complement(17309..18202)
                     /gene="argP"
                     /locus_tag="SI621_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HEI4064775.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18343..18573
                     /locus_tag="SI621_002051"
     CDS             18343..18573
                     /locus_tag="SI621_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064776.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGIANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18629..19288
                     /gene="rpiA"
                     /locus_tag="SI621_002052"
     CDS             18629..19288
                     /gene="rpiA"
                     /locus_tag="SI621_002052"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEI4064777.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19544..20776
                     /gene="serA"
                     /locus_tag="SI621_002053"
     CDS             19544..20776
                     /gene="serA"
                     /locus_tag="SI621_002053"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEI4064778.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21005..21064
                     /gene="ibsC"
                     /locus_tag="SI621_002054"
     CDS             21005..21064
                     /gene="ibsC"
                     /locus_tag="SI621_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HEI4064779.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21165..21767)
                     /locus_tag="SI621_002055"
     CDS             complement(21165..21767)
                     /locus_tag="SI621_002055"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEI4064780.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVRPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21788..21971)
                     /gene="ssrS"
                     /locus_tag="SI621_002056"
     ncRNA           complement(21788..21971)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SI621_002056"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22013..22342)
                     /gene="zapA"
                     /locus_tag="SI621_002057"
     CDS             complement(22013..22342)
                     /gene="zapA"
                     /locus_tag="SI621_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEI4064781.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22510..23088
                     /gene="ygfB"
                     /locus_tag="SI621_002058"
     CDS             22510..23088
                     /gene="ygfB"
                     /locus_tag="SI621_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HEI4064782.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23114..24439
                     /gene="pepP"
                     /locus_tag="SI621_002059"
     CDS             23114..24439
                     /gene="pepP"
                     /locus_tag="SI621_002059"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEI4064783.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVLYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24436..25614
                     /gene="ubiH"
                     /locus_tag="SI621_002060"
                     /gene_synonym="visB"
     CDS             24436..25614
                     /gene="ubiH"
                     /locus_tag="SI621_002060"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEI4064784.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLTLTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25637..>26654
                     /gene="ubiI"
                     /locus_tag="SI621_002061"
                     /gene_synonym="visC"
     CDS             25637..>26654
                     /gene="ubiI"
                     /locus_tag="SI621_002061"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HEI4064785.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSR"
BASE COUNT         6443 a         6512 c         7224 g         6475 t
ORIGIN      
        1 attgattatt ttccagaatc atcaatctca gttatacatt cagcaaaaga ctggcaggaa
       61 gctatcgatt tctcgatggc atcattgctg gataaaaact atatcagcga gaattacatt
      121 caatctatta aagactccac catcagcaat ggtccttatt atattctcgc accaggcgtg
      181 gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa ccgggatgtc tttgacgtta
      241 cttaaacaag gcgtttattt tccggggaat gacgaaccga ttaaattact catcgggctt
      301 tctgctgccg atgccaattc gcacattggc gctattcagg cgttaagtga attactgtgc
      361 gaagaagaaa tactcgaaca actcttaaca gcatcatcag aaaaacaatt agcggacatt
      421 atcagccgcg gataattact ctttcctcta caggataata ttatggaaaa caagtctgct
      481 cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa tggtcatccc caatatcggt
      541 gcttttattg cctggggatt tataacggca ttatttattc ccaccggctg gctgccgaat
      601 gaacatttcg ccaaaattgt tggtccgatg attacctatt tattgcccgt gatgattggt
      661 tctaccggtg gtcatctggt cggcggtaaa cgcggcgcgg tcatgggcgg aattggtacg
      721 attggtgtga tcgtcggcgc agagatcccg atgttccttg gctcgatgat tatggggccg
      781 ctcggtggtc tggtgattaa atatatcgac aagtcgctgg aaaaacgcat acctgctggt
      841 tttgagatgg ttatcaataa cttctcatta ggtatcgcgg ggatgctcct ttgtctgctg
      901 ggttttgaag ttatcggccc ggcggtgtta attgctaata ctttcgtcaa agagtgtatt
      961 gaggcgctgg tacatgcggg ttatctacct ttgctgtcag tcatcaatga accggcgaaa
     1021 gtgcttttcc ttaataatgc gatcgatcag ggcgtctatt atccgctggg aatgcaacag
     1081 gcttcggtta acggtaaatc catcttcttc atggtggcct ccaatccagg tcctggtctg
     1141 gggctgctgc tggcgtttac cttgtttggt aaagggatga gtaaacgttc tgcgcccggg
     1201 gcgatgatta ttcacttcct cggtgggatc cacgaactgt atttcccgta tgtgctgatg
     1261 aagccgctga ccattattgc catgattgcg ggcggtatgt ctggcacctg gatgtttaac
     1321 ttactggacg gtggtctggt ggctggccca agtccggggt ctatctttgc ttacctggca
     1381 ctgacgccga aaggctcgtt cctggcgaca attgccggtg ttacggtagg taccctggtg
     1441 tcctttgcta tcacttcgct gatactgaag atggaaaaaa cggtggaaac gaagagcgaa
     1501 gatgagtttg ctcagtcagc caatgcggtt aaggcgatga aacaagaggg ggcattctcg
     1561 ttaagccggg ttaagcgtat cgcctttgtt tgcgatgcgg ggatgggctc cagcgcgatg
     1621 ggcgcaacca ccttccgtaa acgtctggaa aaagcggggc tggcaattga agtaaaacat
     1681 tacgcgatag aaaacgtgcc tgcggatgcg gatatcgtcg ttactcacgc cagtctggaa
     1741 gggcgcgtga aacgtgtgac ggataaacca ctgatattga ttaataacta tattggcgat
     1801 ccaaaactcg acactttatt taatcaatta accgctgaac ataaacactg attacagagg
     1861 taaaaatgaa aaccaaagtt gctgctattt atggcaagcg ggatgtccgt ctgcgcgaat
     1921 ttgagctgcc agaaattacc gataatgaac tgttagtgaa tgtaatttct gacagcgttt
     1981 gtttatccac ctggaaagcg gcgttactcg gtagtgaaca taaacgcgtc cccgacgatt
     2041 tagaaaatca tccggtcatt accgggcatg aatgtgctgg ggttattgtc gaagtgggta
     2101 aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt
     2161 taccaggcgg gtattcagcg ggctacagct acgaatattt tggcggtaac gccacctata
     2221 tgattattcc cgaaatcgcg attaatttag gctgtgtatt accgtatcac ggctcttatt
     2281 ttgctgcggc ctcgctggcg gaaccgatgt gctgcattat tggcgcttat catgccaatt
     2341 atcacaccac gcaatatgtt tatgagcatc gtatgggcgt caaacctggc ggcaatattg
     2401 cgctactggc gtgtgcaggc ccgatgggaa ttggcgctat cgattacgct attaacggcg
     2461 gcatacaacc gtcgcgggtg gtggtagtcg atatcgacga caaacggctg gcgcaggtgc
     2521 agatgctgct gccggtggaa ctggcggcca gtaaaggcat tgagctggtg tatatgaata
     2581 ctaaagggat gagcgatccg gtccagacgc tgcgggcgtt gacggatgat gccggatttg
     2641 atgacgtatt tgtctatgca gcggtgcctt cagtcgtcga gatggcggat gaattactgg
     2701 cggaagatgg ctgcctgaac ttctttgccg gtccgacgga taaaaacttc aaagtgccgt
     2761 ttaatttcta caacgtccat tacaacagta cgcacgttgt cggcacgtcc ggcggttcca
     2821 cggacgacat gaaagaggcg attgccctta gcgccacggg gcagttacag ccgtcgttta
     2881 tggtgaccca tatcggtggc ctggatgcgg tgccagaaac cgtgctcaat ctgccggata
     2941 tccctggcgg taaaaaactc atttataacg gcgtgaccat gccgctgacc gccattgccg
     3001 attttgccga aaaaggcaaa accgatccgc tgtttaaaga gctggcgcgg ctggttgaga
     3061 aaacgcacgg catctggaat gaacaggccg agaaatatct gctggcacaa tttggcgttg
     3121 atattgggga ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca
     3181 ggcagcgttg gctgcctggc cgcaaaccgg ctgtggcgac aaaaataaaa ttgatggcct
     3241 tgcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct tttcgtgggc aagtggttat
     3301 cggcgaagga gaaattgacc atgcgccgat gctgtggatt ggcgaagatg tgggcaaagg
     3361 cgatgggcca gaagtcgata tcgcggttga cccgattgaa ggcacccgga tggtggcgat
     3421 ggggcagagc aatgcgctgg cggtgatggc cttcgcccca cgaggtagcc tgctacacgc
     3481 gcccgatatg tatatgaaaa aactggttgt taatcggctg gcggcgggcg cgattgatct
     3541 gtccatgcct ctggcggaca acttgcgcaa cgtggcgaaa gcgttaggca aaccgctgga
     3601 caagctgcgt atggttacgc tggataaacc gcgcctgagt gccgcaattg aggaagcaac
     3661 acagctgggc gtgaaggttt ttgccctgcc ggatggcgat gtcgccgcca gcgtgctgac
     3721 ctgctggcag gataatcctt acgatgtgat gtacaccatc ggcggtgcgc cagaaggcgt
     3781 gatttctgcc tgtgccgtta aagcattagg cggggatatg caggccgagc tgattgactt
     3841 ttgtcaggct aaaggggatt acacggaaaa tcggcagatt gcagagcagg agcgcaagcg
     3901 ttgtaaggca atgggcgttg acgttaaccg tgtatactcg ctcgatgaac tggtgagggg
     3961 gaacgatatc ctctttagcg ctacgggcgt gacgggcggc gagttggtga acggtatcca
     4021 acagacggcg aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg
     4081 taatataata gactccctgc attgatggtg atctatcgga agaccaatgg cgacgctgac
     4141 agaagatgat gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac
     4201 tgcgcattct attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa
     4261 cgatgaagag atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga
     4321 cgatattgat gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta
     4381 cgccgatatc acgcattttt cccagttttg gcattacctg aacgaacagg atgaaacgcc
     4441 cggatttgcc gatgacatgg cctgggattt tatcagcaac gtcaatagca taatccgcaa
     4501 tgcgagtctc tatgacgcgt tgaaagcgat gaaattcgcc gatttcgccg tctggtcaga
     4561 ggcgcgtttt agcgggatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa
     4621 ggaattaaca ccgtgaaaat cgaactgacg gtgaatggac taagggtaca ggcgcagtac
     4681 tctgatgatg aaattgaaaa tgtacataaa ccgttgctgc gtatgctggc ggccttacag
     4741 gcggtaaatc cgcagcggcg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa
     4801 tctaccctaa ccaccttctg ggaatatctc gcacaacaag acccggaact gcccgctatt
     4861 cagacactgc cgatggatgg ttttcaccat tacaatagct ggctggatgc gcataaattg
     4921 cgccccttca aaggcgcacc agagacattc aacgttgcga aactggcgga aaatctgtgc
     4981 cgggttgtgg aaggggattg tacgtggccg cagtacgatc gacaaaagca tgatcctgtt
     5041 gaagatgcgt tggacgttac cgctcctctc gtcatcgtcg aaggaaactg gttgttgctg
     5101 gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt tatcaaagct
     5161 cccgcatctg cgttgcggga gcgtctggtg gggcgcaagc tggcgggagg gttatcgctg
     5221 gcagacgcag aagcctttta tgaccgtacc gacgggccga atgttcgccg ggtgctggaa
     5281 gaaagtctgc ctgcaaattt gaccttaatg atgaccgcta cgggtgaata tcgtctgatg
     5341 gattaacgtt cctgattaca ccatcttcca gtcgcaccac acgagagaaa tgacgccggg
     5401 taaatgcggc gtcatgactg attgctacga cgctcgtgcc tcgctggcgg cacttctcca
     5461 gccagctttc aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc tcgtccagta
     5521 gcaatagtgg cggagagagt gcttccaggc aggcgacggc gaccatccgt cgctgcgcac
     5581 tatgcagatc taacggatgc gcgctggcag tgtctgctaa ctgacagcat tgcagggcag
     5641 cattggtacg ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga
     5701 tctcgtcagc aacggtgctg tgaaaaagct ggttttccgc ttcctgaaac agtacgccaa
     5761 tctttgccgc gcgctggcgg tttttcaggt ttttcatcgc ttgttgttgc agcataaccg
     5821 taccggctgt gggcgtaaga agacctgcca ttactcgtaa cagcgtcgat ttccctgcgc
     5881 cattatcgcc ggtcagcgcc agccattcac cttgtttgag ttgcagagaa atgtcgcaaa
     5941 ggcaatccgt tgccgcgcca ggccagcgat aagatatctg atttaacgtt aacatagtgg
     6001 ctggagcgct ccgttttgca attgccacgc ttgctggcag tagttcagga aaggtgtgcg
     6061 atgacgttca aaaagaataa tcagcgaacc acgttcaagc gcccagtgtt gtaagcgctg
     6121 caatagcatt tcgcgggcct gcggcgtcag gcggctaaag gcttcatcaa gaattaagag
     6181 ctttggctgc atcgcgatgg cgcaggcgat gaccacgcgc tgtgtctctc caccggagag
     6241 tgttgccggg tgacgatggc gcaacggctg gcactcggtc agggccaggg cggcgtcaat
     6301 ccgcgccata atctcttttt cagcaagaca cagattttct ggcccaaagg caacttcttc
     6361 ttccacgcta aaggtacagc cggagagctg caaatagggc gattgctgga cgagctggat
     6421 ggtggcggat tgttcattaa gcgggagacg cccaatgggc gtaccgagaa gcgtccccgt
     6481 gccggtgatc tctcctggta gaaagtccgg ataccagccc gccatcagct gcgccagcgt
     6541 acttttgcca ctgccgttgt caccaaagat ggcgaccatc cccggtttga catagtgaaa
     6601 gtcataacag aacggaggat gcgttgagtg tgtcgggcaa tagcgaaact gttctaacgt
     6661 aaccatatcc acgctccgaa ttcagccagc attagcaaga tcatggcgta acgtgctacg
     6721 cgttgtaggg tgctatcggc tggtgcccat aacgtagtgc gcctattgtt gattcgaaat
     6781 gcccgcatat ccagcgccgc accacggaca gcaagatcgt ttagcgcatt gtgtgttagc
     6841 ggaataatta acgcgggcat cgcacgtaaa cgctgatacc agccctcatc cagcggcacg
     6901 ccgcgggcgc actgcgcttc atgaataatc gccaattgcc gttttaactg ctcaacgacc
     6961 aataacggcc cggcaaacag ataagcgacg cccggcggca ggcgagaagc aaacaaagcg
     7021 cggataaaac gttgcacggg gacgtactgc atccacaact gtgaggttga aacaatcgcc
     7081 agaatccgca accagagcgt aatagcatgc gcccaccgtt ctggagagcg tggcgtgccg
     7141 cttagccatt ctgtcagcca gccaccgtgc accagccaca gtccagcccc cagcgagaac
     7201 atcagccaga cgacatattt tgcccgccgc cgcgtggcct ttagcgcaat aaggcagaaa
     7261 aaggtcgccg cgctatagac tggcaatatt gtctgtgtgg gcaggataag cgtggtgcag
     7321 gccgccagcg cccacagcgt taaggatgta aaaggatgca tttagcgcac ggcagccatt
     7381 gccggaaaat ggcgcgtggt gcgaatcggc agctgacgca gtaaaagcca gactatcacc
     7441 gctgtaagaa ttttatcgac caggttcgcg cctatcaccg taatcgccac cgactccacc
     7501 aggttttgcc ccatcgagtg catccaggca acgaacaaat cggctccgct tcctgtcacg
     7561 ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg ttaccgcaag cgtaataatc
     7621 acgccgctga caacgacttt tggcagggtg cgaaaccaac ccgctcgcgc cagccaaccg
     7681 gcgaccaggc cgatgaccat cgcgacaggc gcaaatgcgg cggcgatcgg gtcggtcagc
     7741 aatccccaga gtaaattggt cagtaaaccg gtcagcatcc cgattaccgg accaagcagt
     7801 accgcgctaa taagtgtgcc gatggagtcg agaaaaatag gtaatttaac catgctggcc
     7861 agttgcccgc cgatcatatt gatggcgata gagataacga tcaacaccag tgcctgactg
     7921 gaaaagtggc gacgcgccat aagaaaacct ctctgaaaat agttatttaa cgggggaaat
     7981 atgcaactcg tcgtaaccag aaagcgcgca aagctggcgg ctaaacgcca gttcctcaag
     8041 gcagccgatc accagttcaa gcgtggtccg cacggtacag ccgggcatca tatgaccgcc
     8101 aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga tgttcaccac tctgttccag
     8161 cgtgccgttc aatgcgatga cttcgaactt accgcttagt agggtcgtgt tttcttgccc
     8221 ggcatagcgt aaggcgacgt cagtcagact gccggtacaa cctgcaatcc acgcggcgtg
     8281 aagttgttgt tgctgcgcga aggcgcgtaa ttgggaaaga acttcctgcc cggggagcag
     8341 gcgcagcgcg taaaagcgtg cggaggaaga atatgggtag ggcaatgtca tcattggagt
     8401 actccgaaac tcggacgtat tgcggataat atcgacgctg catcagtcat attctgatat
     8461 aagtcagggt ttgtgacggc acaacgaaaa gattttgcta cgcttattcg tgattctgca
     8521 aagtggaata aattccacag gggcggggct gaaaacgtga cgtaagtcag ataattgtct
     8581 tctttgactg gacaatcatt ccttttattc cacgtttcgc ttatcctagc tgaagcgttt
     8641 cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag gtaaggtttc
     8701 cccgaacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa ccttgcagga
     8761 gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt aatgtggttc
     8821 gtgctttgta tgaatccgga cgccgtgcgg aaattaccgt ggtggcaatc aacgagctgg
     8881 cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc cgtttttcat
     8941 gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc cgtgtattgc
     9001 atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta gtcctcgact
     9061 gcaccggcgt atatggctcc cgcgagcatg gtgaggcaca cattgccgcc ggggctaaaa
     9121 aagtgctctt ttcacatcct ggcagtaacg atctcgacgc gaccgttgtt tatggcgtca
     9181 atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt accacgaatt
     9241 gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc ggcactgtga
     9301 ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat ccagacctgc
     9361 gtcgcactcg ggcagccagt cagtcgatca ttccggtcga tactaaactg gccgccggta
     9421 tcacacgatt ttttccgcaa tttaacgatc gctttgaggc gattgcggta cgtgtgccaa
     9481 ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta aaagccaatg
     9541 aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata gttgactata
     9601 cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc attgtcgatg
     9661 gcactcaaac ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg tgcgataacg
     9721 aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact gttgctttca
     9781 ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt caccatgtct
     9841 gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg cgcggatctg
     9901 aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc ttctctgccg
     9961 accatcgaac tggccctgaa acaaggcgca aaagtgatgg taacttccca cctgggtcgt
    10021 cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa ctacctgaaa
    10081 gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt tgacgttgct
    10141 gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa gaaagacgac
    10201 gaaactctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga cgcattcggt
    10261 actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga cgttgcgtgc
    10321 gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa agaacctgct
    10381 cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac cgttctggac
    10441 tccctgtcta aaatcgctga ccagctgatc gttggtggtg gtattgctaa cacctttatc
    10501 gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt tgacgaagct
    10561 aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgcgt agcaaccgag
    10621 ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc tgacgagcag
    10681 atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa gaatgcgaaa
    10741 accattctgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg caaaggtact
    10801 gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct ccatcgctgg cggcggcgac
    10861 actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat ctccactggc
    10921 ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc gatgctcgaa
    10981 gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt tcagcgcgct
    11041 tttagagcac gcactttttc atactctaaa taattcgagt tgcaggaagg cgacaagaga
    11101 gtgaatcccc aggagcttac ataagtaagt gattggggtg agcgaacgca gacgcagcac
    11161 atgcaacttg cagtatgacg agtataaggc ccgacgatac aggacaagag acatgtctaa
    11221 gatttttgat ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga aagttttcca
    11281 ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta ctgactccat
    11341 caacgccgta ctggaaaccg cagctaaagt taaagcgccg gttatcgttc agttctccaa
    11401 cggtggtgct tcttttatcg ctggtaaagg cgtgaaatct gacgttccgc agggtgctgc
    11461 tatcctgggt gcgatctctg gtgcgcatca cgtacaccag atggctgaac actacggtgt
    11521 tccggtgatc ctgcatactg accactgcgc gaagaaactg ctgccgtgga tcgacggtct
    11581 gctggacgcg ggtgaaaaac acttcgctgc taccggtaag ccgctgttct cttctcacat
    11641 gattgacctg tctgaagaat ctctgcaaga gaacattgaa atctgctcta aatacctgga
    11701 gcgcatgtcc aaaatcggca tgactctgga aatcgaactg ggttgcaccg gtggtgaaga
    11761 agacggcgtg gacaacagcc acatggacgc ttctgcactg tacacccagc cggaagacgt
    11821 tgattacgca tacaccgagc tgagcaaaat cagcccgcgt ttcaccatcg ctgcgtcctt
    11881 cggtaacgta cacggtgttt acaagccggg taacgtggtt ctgactccga ccatcctgcg
    11941 cgactctcag gaatatgttt ctaagaaaca caacctgccg cacaacagcc tgaacttcgt
    12001 attccacggt ggttccggtt ctactgctca ggaaatcaaa gactccgtaa gctacggcgt
    12061 agtgaaaatg aacatcgata ccgataccca atgggcaacc tgggaaggtg ttctgaacta
    12121 ctacaaagaa aacgaagctt acctgcaagg ccagctgggc aacccgaaag gcgaagatca
    12181 gccgaacaag aaatactacg atccgcgcgt atggctgcgt gccggtcaga cttcgatgat
    12241 cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc gacgttctgt aagatattcc
    12301 tttctgctta tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc aaggtgatac
    12361 agatgagaaa tctgtgatct atttggcaaa attatgcttt attgtttacc cttgtcagac
    12421 tgcccgtcat aaggcggcgg agtgtatttc tccattttga gtcagttgaa aaggaatatt
    12481 gaatggaaga tttgaatgtt gtcgatagca taaacggcgc gggaagctgg ctggtggcta
    12541 accaggcgct gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg atcatcatcg
    12601 ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg atctcccgta
    12661 aaatcgatgc cactgttgct gattttcttt ctgcattagt ccgttacggt attatcgcct
    12721 ttacgctaat cgctgcactg gggcgcgtgg gcgtacaaac agcgtcagtc attgctgtac
    12781 tcggtgccgc aggtttagct gttggtctgg ctttgcaggg gtcactgtct aacctggccg
    12841 ctggcgtatt acttgtcatg ttccgcccgt tccgtgccgg tgaatatgtt gacctcggcg
    12901 gcgtagccgg tactgtgctg agtgtgcaga ttttctccac caccatgcgt actgcagacg
    12961 gtaaaattat cgttattccg aacggtaaaa ttattgccgg gaatattatt aacttctccc
    13021 gcgagccagc tcgccgtaac gaatttatta ttggcgtggc gtatgattcc gatatcgatc
    13081 aggttaagca gatcctgacc gatattatcc agtctgaaga tcgcattttg aaagatcgcg
    13141 aaatgactgt gcgtctgaac gaacttggtg catcgtcgat taatttcgtg gtccgagtct
    13201 ggagcaacag cggcgatctg caaaacgtgt actgggatgt gctggagcgt attaaacgtg
    13261 aatttgatgc cgccggtatc agcttcccgt acccgcaaat ggatgtgaac tttaagcggg
    13321 tgaaagaaga caaagctgcg taatcaacgc tgaggccaga taatactctc tggcctctct
    13381 cttattagtt tttctgattg ccaattaata ttatcaattt cctctaataa caatcccgcg
    13441 atatagtctc tgcatcagat acttaattcg gaatatctaa cgtgttttct tattactttc
    13501 aaggtcttgc acttggggcg gctatgatcc taccgctcgg tccacaaaat gcttttgtga
    13561 tgaatcaggg cattcgtcgt cagcaccaca ttatgattgc cttactttgt gctatcagcg
    13621 atttggtcct gatttgtgcg gggatttttg gtggcagcgc gttattgatg cagtcgccgt
    13681 ggttgctggc gctggtcacc tggggcggcg tagccttctt gctgtggtat ggttttggcg
    13741 cttttaaaac agcaatgagc agtaacattg agttagccag tgccgaagtc ctgaaacaag
    13801 gcagatggaa aattatcgcc accatgttgg cagtgacctg gctgaatccg catgtttacc
    13861 tggatacttt tgttgtgctg ggcagccttg gcgggcaact tgatgtggaa ccaaaacgtt
    13921 ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt ctttggtctg gctattctcg
    13981 cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca gcgcattatc aatttggtag
    14041 tgggatgtgt tatgtggttt attgccttgc agctggcgag agacggtatt gctcatgcac
    14101 aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc taagcttgcc
    14161 tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa
    14221 agttatcgcc ctggcggcat taatgggtat tagcgggatg gcagcgcagg ccaacgaatt
    14281 gccggatgga ccgcatattg tcacctccgg tacggcaagc gtcgatgcgg tgccagacat
    14341 tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctactg ccaagaaaca
    14401 ggcagatgaa cgcgtcgcac aatacatttc cttccttgag ctcaatcaga tcgcgaaaaa
    14461 agatatcagc tcagcgaact tacgcactca gccagattat gattatcagg atggtaaaag
    14521 tatcctgaaa ggctaccgcg cagtgagaac ggtggaagtc acgctccgtc agttagacaa
    14581 actgaattcc ttgctggacg gcgcgctaaa ggcgggtctt aacgaaattc gttctgtgtc
    14641 gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag cgattgataa
    14701 cgcgattcat caggcacagg aactggcgaa tggctttaat cgcaaactgg ggccggtata
    14761 tagcgtgcgt tatcatgttt ccaactatca gcccagccca atggtgcgga tgatgaaagc
    14821 tgatgctgca ccggtgtccg cacaggaaac ttacgagcag gccgctattc agtttgatga
    14881 tcaggtcgat gtagtcttcc agttagaacc tgtggatcaa caacctgcta aaacacctgc
    14941 agcacaataa tacttgtagc ccgacgtatt cgtcgggcta atgcctttac ccgatattgc
    15001 ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt
    15061 tcatcaagga taggctgact gaaacggtta agcggacaag gatgcgggag aggggggatg
    15121 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    15181 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggcaaaag cattgccgcg
    15241 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    15301 aaagccggac aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    15361 gataatgaaa tccataactc aggacgtcgt taacgagaaa ttgtgatagg catagataat
    15421 acttatccga caattatttt tgatcagttg attagtctcg gagataagta cgaaggcgtg
    15481 accgcccagc cagttgagtt cagcgaaaat ggcgttatcg ataatctttt tgaccgtcag
    15541 ttagatttta ttatctctcc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    15601 actatcagtg aactaccgcc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    15661 cgacaggagc aagaactgct tcaggagctg ccctggctac aaatgcgctt tcagaatcgg
    15721 gccaatttcg aagcaatgat tgatgcgaat atgcgtcctt gtgggatcaa tcccaccatt
    15781 atttatcgtc cctacagttt tatggctaaa atcagcgccg tggagcgggg acattttctg
    15841 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    15901 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    15961 accgccacga tgcttcagca tattgctgaa gatcgtgacg gggcaaacca ttaacccagc
    16021 atcgatccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    16081 atatgtccgc cggacgcatt ttccagatag cgatgcagat aatcccgaca cataggatgt
    16141 gcacaatgat caatggtggt gcggataacg ttgttatacg gatcctgtgc gccaaatcca
    16201 ctgaccgctt cgctccataa ataacgtgcc gcacgcaaca tggcggcgtt cataaacaga
    16261 tccatgccaa tgccgaagaa aaacgacagg cgagggacga agtcatcaat tttcagcccg
    16321 gcggagattg ctgctttgat gtactcaatc ctatcggcga gcgtaaatgc tacctgctgt
    16381 acgcagttgg cacccgcttc acccatgtgg taactgctga tactgatggt attaaatcgc
    16441 ggcatgttgc cggaacacca ggcgatgatg tctgcgataa tacgcattga cggttttggc
    16501 gggtaaatat aggtattgcg gcagaggtac tctttgagga tatcgttctg aatggtgcca
    16561 gtcagtttat cggatgtaac accttgctct tctgcggcga cgatataaaa cgccagtact
    16621 ggtagcactg cgtcattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg
    16681 aacagctctt tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc gacgtcgccc
    16741 gccacgcgtg ggttatcgaa gtcgtagcct cggtgggtgg caaggtcaaa cgcaacggaa
    16801 agaccttttt gcccggcggc caggttacgg cgataaaaag cgttggactc cttttgctgt
    16861 cgagaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag tggcacgcgg
    16921 gccacgaacg tagggtggca aaccaggaag ggtacccgtc acctccagat tatcgagatc
    16981 ggcttcggta tacagcggct tgatggcgat cccttctgcg gtttgctgaa ccagcgagtc
    17041 gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccaca ccagcacgtt
    17101 agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgctt acggtgagct
    17161 ttttacgtgg gtttgtcgcc agcaaaaaaa tgcattcaga cgtcgttttt tgccgcctga
    17221 gatttgtcta ttttgtaatg tgattcaagg aatacaaaaa gaagtgagga taacgcctga
    17281 tatgtgctat caggcgttat ttgatggatt aatcctgacg aagaactttg tgaccataat
    17341 cgagcaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt
    17401 agagcatccg gcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggccagtt
    17461 ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctgacga gcaagttgca
    17521 cgaacgcttc cgaagaatta acgatatggc agggtacgct gccaggaggc agatcgaagt
    17581 tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc actggcgctt
    17641 tcagtaatgc cgaacgcgtt acgccgttag ggaaatattt ttcggcaaac ggttttgagc
    17701 tgacgaacag atagtcgagc gcaccgagtt tatcgacaag acaactcggc agtgcctgat
    17761 gttgaatact cactgcgccg accacttcgc cgcgacgcag acgttcctga gtgcgggttt
    17821 catcttctac ctgcaagttg aggcggatgg gcgaatcagc caacacagga gccagtgccg
    17881 gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc agcggagtcg
    17941 aaccggtttg ttcatcgccc agccactctt cttccagcag ctccacctgg cgtagtagcg
    18001 ccaacagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcact aacaacggct
    18061 gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca
    18121 gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg
    18181 ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac
    18241 tatgacacaa ttttgtgtcg ggttgcatat accgaagccg ttggcgggta acatttgtat
    18301 cagtggttaa cgcgcagctt aaaagatcag agatttgcgc taatgcattt cgctcagcga
    18361 gttcgcgccc tggttgtgtt gaatggcgtg gcgttattgc ctcagtttgc ctgtaaacag
    18421 gggattgcga acggggaact ggtgcgcctg ttcgcaccgt ggagcggcat acccagaccg
    18481 ttgtatgctt tatttgcggg gcgaaagggg atgcccgcca ttgcgcgata ttttatggat
    18541 gagttaacca cgcggcttgc caacggggtc tgaatcgctt tttttgtata taatgcgtgt
    18601 gaaatttcat accacaggcg aaacgatcat gacgcaggat gaattgaaaa aagcagtagg
    18661 atgggcggca cttcagtatg ttcagcccgg caccattgtt ggtgtaggta caggttccac
    18721 cgccgcacac tttattgacg cactcggtac aatgaaaggc cagattgaag gggccgtttc
    18781 cagttcagat gcttccactg aaaaactgaa aagcctcggc attcacgttt ttgatctcaa
    18841 cgaagtcgac agccttggca tctacgttga tggcgcagat gaaatcaacg gccacatgca
    18901 aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga
    18961 aaaatttatc tgtattgcag acgcttccaa gcaggttgat attctgggca aattcccgct
    19021 gccagtagaa gttatcccga tggcacgtag tgcagtggcg cgccagctgg tgaaactggg
    19081 cggtcgtccg gaataccgtc agggcgtagt gaccgataac ggcaacgtga tcctcgacgt
    19141 ccacggcatg gaaatccttg acccgatagc gatggaaaac gccataaatg cgattcctgg
    19201 cgtggtgact gttggtttgt ttgctaaccg tggcgcggac gttgcgctga ttggcacacc
    19261 tgacggtgtc aaaactattg tgaaatgatc tgacggggga acctcccccg ttaaaaaaat
    19321 tctcttcatt aaatttggtg acatgtgtca cgtttttacc aggcgattgt cgattgctct
    19381 aaataaatcc tctaaaccag catattcatc caagaaatac ttttgcgtga tatttcctca
    19441 acatcgcgac gcaaacgttc atattgccgc aatattattt tttgatatgt tgaaaggcgg
    19501 atgcaaatcc gcacacaaca tttcaaaaga caggattggg taaatggcaa aggtatcgct
    19561 ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg cgctggaaag
    19621 ccttcgtgca gcaggttaca ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca
    19681 attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta cccatctgac
    19741 tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt ggctgtttct gtatcggaac
    19801 aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt
    19861 ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc tattgcgcgg
    19921 cgtgccggaa gccaatgcta aagcgcatcg tggcgtgtgg aacaaactgg cggcgggttc
    19981 ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg gtacgcaatt
    20041 gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg aaaacaaact
    20101 gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata tgagcgatgt
    20161 ggtgagtctg catgtaccag agaatccgtc caccaaaaat atgatgggcg cgaaagagat
    20221 ttcgctaatg aaacccggct cgctgctgat taatgcttcg cgcggtactg tggtggatat
    20281 tccagcgctg tgtgacgcgc tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt
    20341 cccgacggag ccggcgacca atagcgatcc atttacctct ccgctgtgtg aattcgacaa
    20401 tgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga atatcggcct
    20461 ggaagttgcg ggtaaattga tcaagtattc tgataatggt tcaacgctct ctgcggtgaa
    20521 cttcccggaa gtctcgctgc cactgcacgg tggtcgtcgt ctgatgcaca tccacgaaaa
    20581 ccgtccgggt gtgctgactg cgctcaacaa aatttttgcc gagcagggcg tcaacatcgc
    20641 cgcgcaatat ctgcaaactt ccgcccagat gggttatgta gttattgata ttgaagccga
    20701 cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta ccattcgcgc
    20761 ccgtctgctg tactaaatcc ctttctctga aaatcaacgg gcaggtcact gacttgcccg
    20821 ttattttatc ccttctccac accgttgccg cgttgcgcat tctactttgc gagtctgctc
    20881 cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg
    20941 cgggactgta cagggctgta acggtaaagc cctcaccgaa gcgagggctt gaaggagaag
    21001 ggttatgatg cgacttgtca tcatactgat tgtactgtta ctcataagtt tcagcgctta
    21061 ttaacagtca gtcacagggg aggagcaatc cccccttacc cttactcact aaattaggtt
    21121 aaagaatcaa cgatgtcaat cagggcgatg caggtgtgtc gcccttacca ctcccagact
    21181 ttcgacggtg taaccaccgc aggcagtggg atatcccact cttcaacggg gagtttttcc
    21241 accaactgac aatcatgcgc ataacccacc ggttgcattt tatagtgctg ccagttttgt
    21301 aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc atcaaaggcg
    21361 accagcggtg taatcaacac gtctaatcgg gaaaggggtc gcacgtcacg cacatccagt
    21421 tttggctcat ggatcttcaa cttgttcatc accagttcgc tttgtggatg gtaattcagg
    21481 aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg cttaccggcg
    21541 cgccagagtt gttctatgag tggctgggtg tcgagttcgc catcaaaaga gaggaataca
    21601 gcgaccgtat gtgccatcac caccggggga taagtcatca tccgggtagc ggcttgttga
    21661 cccatttcct gctgttgttc cggcgttaac gcacgacgac gttgccgaat cattttgcgg
    21721 atttcttgtc gggataatgt cagtgggagt tctggtaatt gcgtcatggc tggtgccaga
    21781 taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct tggttcaagg
    21841 tgaatgtgtc gtcgcagttt taaggcttct cggacggacc gagcatgctc accaaccgcg
    21901 gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc ttgcgaacat
    21961 ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag tgtcattcaa
    22021 agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt tctatggtct
    22081 gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc ttcgcttttt
    22141 cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt tcagtatttg
    22201 tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc gctgcctgat
    22261 tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca aaaatttgga
    22321 tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg ccttcgtctt
    22381 tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag agacaagccc
    22441 ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc cctttgacga
    22501 cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa ccagtatctg
    22561 aaccaacaag ggacgggtct gacccctgct gagatgcatg gtttaatcag tgggatgata
    22621 tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac gaacgaaggc
    22681 atggctttcg gtcatgagct ggcacaggca ctgcgcaaaa tgcactctgc caccagcgat
    22741 gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga tgatgtcagc
    22801 gttttcgatc gggctgatgc gctggctggt tgggtcaatc acttcctgct tggtcttggc
    22861 gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat cgacgatctg
    22921 cgtaacatcg cgcagttggg ttacgacgaa gacgaagatc aggaagagct tgaaatgtcg
    22981 cttgaagaga tcatcgagta cgtccgtgtt gccgcgctgt tatgccacga cacctttact
    23041 catccgcaac cgactgcgcc agaagtacaa aaaccgactc tacactaaaa acaaaaaatg
    23101 taaggagagt gttatgagtg atatatcccg gcaagaattt cagcgtcgcc gtcaggccct
    23161 ggtagagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag aagtaacacg
    23221 tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact tcaccggctt
    23281 taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata accacagcgt
    23341 tttgtttaac cgcgttcgcg acctgacggc agagatctgg tttggtcgtc gcttaggcca
    23401 ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg aaatcaatca
    23461 gcaactttat caactactta atggtctgga tgtgctttac cacgcccagg gcgaatatgc
    23521 atatgctgat gaaatcgtga acagcgcgct tgagaaactg cgtaaaggtt cgcggcaaaa
    23581 tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt
    23641 caaatcacca gaagagattg ccgtacttcg ccgcgcgggt gaaatcaccg cactggcgca
    23701 tacacgggcg atggaaaaat gccgtccggg gatgttcgag taccatctgg aaggcgaaat
    23761 tcaccacgaa tttaaccgcc acggtgcgcg ttacccttcc tataacacca ttgttggcag
    23821 cggtgaaaac ggctgcattt tgcactacac cgaaaacgag tgtgaactgc gtgacggtga
    23881 cctggtgttg attgacgcgg gttgtgaata caaaggttac gctggcgaca ttacccgcac
    23941 cttcccggtc aacggcaaat tcactcaggc tcagcgtgaa atctatgaca ttgtgctgga
    24001 gtctcttgaa accagcctgc gcctgtatcg tccgggaact tccattcagg aagtcactgg
    24061 tgaagtggtg cgcatcatgg ttagcggtct ggtaaaactc ggcatcctga aaggtgatgt
    24121 tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc ttagccactg
    24181 gttaggactg gatgttcatg acgttggtgt ttatggtcag gatcgctcgc gtattctgga
    24241 accgggtatg gtactgaccg tagaaccagg actgtatatt gcgccggatg cagaagtgcc
    24301 agaacagtat cgcggtatcg gcattcgtat tgaagacgat attgtgatta ccgaaaccgg
    24361 taatgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc
    24421 agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg
    24481 gcgctggcta tttcccggtt aagtcacggg gcgctgccag tacatttgat tgaagcgacc
    24541 gcgccagagt cacatgcgca tccgggcttt gatggacgag ctattgcgct ggcggcgggt
    24601 acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggctgattg cgcaactgcc
    24661 atcaccaccg tgcatgtcag cgatcgtggt cacgctggat ttgtcaccct cgccgcagaa
    24721 gattaccaac tggcggcgct ggggcaggtt gtcgaattgc acaatgtcgg gcaacggctg
    24781 tttgcattgc tgcgtaaagc acctggcgta acgctgcatt gccctgatcg cgtggctaac
    24841 gttgcccgta ctcagagcca cgttgaagtg acgctggaga gtggcgagac gctgacgggc
    24901 cgcgtgctgg tagccgctga tggcacccat tcagcgttag ccaccgcgtg cggtgttgac
    24961 tggcagcagg agccttacga acaactggct gtgattgcca acgttgcgac ttccgttgcg
    25021 catgaagggc gcgcttttga acgctttacg caacatggcc cgctggcgat gttgccgatg
    25081 tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga agaggtgctg
    25141 tcgtggagcg acgagaagtt ctgccgtgaa ctccagtcgg cctttggctg gcgactgggg
    25201 caaattaccc acgccggaaa acgtagtgct tatccgctga cgttaacccg tgccgccaaa
    25261 cctattaccc atcgtaccgt gctggtgggg aatgcggcgc aaactctgca tcccatcgcc
    25321 gggcaaggat ttaacctcgg tatgcgtgat gtgatgagtc ttgcagaaac cctgactcag
    25381 gcgcaggaac gcggagaaga catgggggat tacggcgtat tgtgccgtta tcagcagcgt
    25441 cgacagcgcg atcgcgaagc aaccatcggc gtcacggaca gccttgtaca tctttttgcc
    25501 aaccgttgga cgccgctggt tgtcgggcgc aacatcgggc tgatgacgat ggaattattt
    25561 accccggcac gcgatgtgct ggcgcagcgc actcttggtt gggtggcgcg ttgaaggcgg
    25621 ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg gcatggtcgg
    25681 gctggcggtt gcctgcggct tacagggtat cggcttacgc gttgccgtac tggagcagcg
    25741 cgtaccggaa cctctggcgg cggatgcacc accacaactg cgcgtttcgg ctatcaatgc
    25801 cgccagcgaa aaattactca cccgtcttgg cgtctggcaa gacattctct ctcgtagggc
    25861 ctgttgctat cacggcatgg aagtgtggga caaagacagc tttggtcaca tctcgtttga
    25921 cgatcaaagc atgggctata gccatcttgg gcatatcgtt gaaaactcag tgattcacta
    25981 cgcgctgtgg aacaaagcgc agcagtcgtc agatatcact ctgttggccc ccgcagaatt
    26041 acagcaggtc gcctggggag aaaatgaaac cttcctgacg ttaaaagatg gcagcatgtt
    26101 aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc tggttgcgca acaaagccga
    26161 tattccgctg actttctggg attatcagca tcacgcgctg gtggcgacca ttcgcacgga
    26221 agaaccgcat gatgcggtgg cgcgacaggt ttttcatggc gaaggcattc tggccttttt
    26281 gccgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag aggaagcgca
    26341 gcggatgcag caggcaggcg aagacgaatt taatcgcgcg ttaaatatcg cttttgataa
    26401 tcgcctgggc ttatgcaagg ttgaaagtga gcgcctggtg ttcccgctaa cggggcgtta
    26461 tgcgcgccag tttgccgcgc accgtctggc gctggtgggc gacgccgcgc ataccattca
    26521 cccgttggcg gggcaggggg taaatctcgg ttttatggat gctgcagagc tgattgctga
    26581 actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc tgcgtcgcta
    26641 tgagcgtagc cgca
//
LOCUS       DARNVM010000055        26550 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.055, whole genome shotgun sequence.
ACCESSION   DARNVM010000055 DARNVM010000000
VERSION     DARNVM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.055"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..653
                     /locus_tag="SI621_002062"
     CDS             <1..653
                     /locus_tag="SI621_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEI4064786.1"
                     /translation="DANGGDVLARILYGTRISVLFGLMLTLCSSVMGVLAGALQGYYG
                     GKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSLVGVVRAEFL
                     RTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTLTSLDFLGFG
                     LPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDAFDPNKAV"
     gene            655..2244
                     /gene="yejF"
                     /locus_tag="SI621_002063"
     CDS             655..2244
                     /gene="yejF"
                     /locus_tag="SI621_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HEI4064787.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     NVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(2248..2592)
                     /locus_tag="SI621_002064"
     CDS             complement(2248..2592)
                     /locus_tag="SI621_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064788.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(2925..4115)
                     /gene="bcr"
                     /locus_tag="SI621_002065"
     CDS             complement(2925..4115)
                     /gene="bcr"
                     /locus_tag="SI621_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HEI4064789.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIEQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(4143..4838)
                     /gene="rsuA"
                     /locus_tag="SI621_002066"
     CDS             complement(4143..4838)
                     /gene="rsuA"
                     /locus_tag="SI621_002066"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HEI4064790.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            4987..6747
                     /locus_tag="SI621_002067"
     CDS             4987..6747
                     /locus_tag="SI621_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEI4064791.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRAPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            6872..7156
                     /gene="rplY"
                     /locus_tag="SI621_002068"
     CDS             6872..7156
                     /gene="rplY"
                     /locus_tag="SI621_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEI4064792.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(7295..8302)
                     /gene="yejK"
                     /locus_tag="SI621_002069"
     CDS             complement(7295..8302)
                     /gene="yejK"
                     /locus_tag="SI621_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HEI4064793.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            8484..8711
                     /locus_tag="SI621_002070"
     CDS             8484..8711
                     /locus_tag="SI621_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HEI4064794.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSIHEDKAH"
     gene            8731..10491
                     /gene="pbgA"
                     /locus_tag="SI621_002071"
     CDS             8731..10491
                     /gene="pbgA"
                     /locus_tag="SI621_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HEI4064795.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            10566..10642
                     /locus_tag="SI621_002072"
     tRNA            10566..10642
                     /locus_tag="SI621_002072"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10600..10602,aa:Pro,seq:ggg)
     gene            complement(10744..13335)
                     /gene="yejO"
                     /locus_tag="SI621_002073"
     CDS             complement(10744..13335)
                     /gene="yejO"
                     /locus_tag="SI621_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HEI4064796.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSILNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANEPTIKGGRLIIEKDGITVFAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKDGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVNYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYSSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWSF"
     gene            13655..14302
                     /gene="narP"
                     /locus_tag="SI621_002074"
     CDS             13655..14302
                     /gene="narP"
                     /locus_tag="SI621_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HEI4064797.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(14338..15390)
                     /gene="ccmH"
                     /locus_tag="SI621_002075"
     CDS             complement(14338..15390)
                     /gene="ccmH"
                     /locus_tag="SI621_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HEI4064798.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15387..15944)
                     /gene="dsbE"
                     /locus_tag="SI621_002076"
     CDS             complement(15387..15944)
                     /gene="dsbE"
                     /locus_tag="SI621_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HEI4064799.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(15941..17884)
                     /gene="ccmF"
                     /locus_tag="SI621_002077"
     CDS             complement(15941..17884)
                     /gene="ccmF"
                     /locus_tag="SI621_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HEI4064800.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            complement(17881..18360)
                     /gene="ccmE"
                     /locus_tag="SI621_002078"
     CDS             complement(17881..18360)
                     /gene="ccmE"
                     /locus_tag="SI621_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEI4064801.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(18357..18566)
                     /gene="ccmD"
                     /locus_tag="SI621_002079"
     CDS             complement(18357..18566)
                     /gene="ccmD"
                     /locus_tag="SI621_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEI4064802.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVAMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(18563..19300)
                     /gene="ccmC"
                     /locus_tag="SI621_002080"
     CDS             complement(18563..19300)
                     /gene="ccmC"
                     /locus_tag="SI621_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HEI4064803.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(19342..20004)
                     /gene="ccmB"
                     /locus_tag="SI621_002081"
     CDS             complement(19342..20004)
                     /gene="ccmB"
                     /locus_tag="SI621_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEI4064804.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGVIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(20001..20618)
                     /gene="ccmA"
                     /locus_tag="SI621_002082"
     CDS             complement(20001..20618)
                     /gene="ccmA"
                     /locus_tag="SI621_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEI4064805.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(20637..21239)
                     /gene="napC"
                     /locus_tag="SI621_002083"
     CDS             complement(20637..21239)
                     /gene="napC"
                     /locus_tag="SI621_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HEI4064806.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(21249..21698)
                     /gene="napB"
                     /locus_tag="SI621_002084"
     CDS             complement(21249..21698)
                     /gene="napB"
                     /locus_tag="SI621_002084"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEI4064807.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(21695..22558)
                     /gene="napH"
                     /locus_tag="SI621_002085"
     CDS             complement(21695..22558)
                     /gene="napH"
                     /locus_tag="SI621_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HEI4064808.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTIPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAAKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(22545..23240)
                     /gene="napG"
                     /locus_tag="SI621_002086"
     CDS             complement(22545..23240)
                     /gene="napG"
                     /locus_tag="SI621_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HEI4064809.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(23247..25733)
                     /gene="napA"
                     /locus_tag="SI621_002087"
     CDS             complement(23247..25733)
                     /gene="napA"
                     /locus_tag="SI621_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HEI4064810.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(25730..25993)
                     /gene="napD"
                     /locus_tag="SI621_002088"
     CDS             complement(25730..25993)
                     /gene="napD"
                     /locus_tag="SI621_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEI4064811.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(25983..26477)
                     /gene="napF"
                     /locus_tag="SI621_002089"
     CDS             complement(25983..26477)
                     /gene="napF"
                     /locus_tag="SI621_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HEI4064812.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT         6426 a         7452 c         6593 g         6079 t
ORIGIN      
        1 cggatgccaa tggcggcgat gtgctggcac gtattctcta tggcacgcgg atctcagttc
       61 tgtttggcct gatgctgacc ctctgctcca gcgtgatggg cgtgctggcg ggggcgctac
      121 aaggctatta cggcggtaaa gtcgatctat gggggcaacg ttttattgaa gtatggtcgg
      181 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
      241 ggctgttggc aattaccgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
      301 agtttttacg tacccgtaat ttcgactaca tccgcgcggc gcaggcgctt ggcgtcagcg
      361 atcgcagtat catcctgcgt catatgttgc caaatgccat ggtcgctacc ctcacctttt
      421 taccgtttat tttatgtagt tcgatcacca ccctgacctc actcgatttc ctcggcttcg
      481 gtctgccgct cggttcaccg tcactcggcg aactgctgtt acaagggaaa aataaccttc
      541 aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattgttg tctttgctga
      601 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
      661 caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt
      721 acagtagtca atgatgtttc acttcagatt gaggctggcg aaacgctggc gctggtgggt
      781 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
      841 ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc
      901 gatcaaacgt tacgcggtgt acgcggtaat aagattgcca tgatttttca ggaaccgatg
      961 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
     1021 cgcgggatgc gtcgggaagc ggctcgtggc gaaattctga actgccttga tcgcgtcggt
     1081 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
     1141 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
     1201 ccgaccaccg cgctggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg
     1261 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
     1321 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
     1381 acgctatttg cctcacccac tcatccttac acacaaaagt tactcaacag tgaaccgtca
     1441 ggcgacccgg tgccattgcc agaacctgcc tcaacgttgc tgaatgttga acagcttcag
     1501 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
     1561 aaaaacatca gttttacgct acgagcgggt gaaacactgg gtttagtggg cgagtccggt
     1621 tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttaattctca gggcagcatc
     1681 atctttgacg gtcagccact gcaaaattta aatcgccgcc agctgttacc tattcgtcat
     1741 cgcattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
     1801 caaattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
     1861 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
     1921 ccggcggagt tctctggtgg tcagcggcaa cgtattgcga ttgccagggc gttaattctt
     1981 aagccctcgc tgatcattct tgatgaacca acatcatcgc tcgacaaaac ggtacaggcg
     2041 caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
     2101 agtcacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
     2161 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg
     2221 cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaga agggttcggc
     2281 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
     2341 aaacaggcac ggctcacctt cacactccag taccgaacat ggaacttcga tgtcgcttaa
     2401 cgcctggctg agtttgtaca tcaccgacca tgcattatca ccatccgggc taagcaattt
     2461 aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
     2521 acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga ttgagagttg
     2581 taatgaagtc acttattttc cccggaagca catttacttc acaatttgtt tacatcaatt
     2641 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgccagcg
     2701 tggcctctat ggctctgatt taaataatat ctgcgggcgt ggtcacgttt gcggggctat
     2761 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
     2821 taaattgatt ttacataaaa taaacatata tcgggggaat gatggatttg tggttgacat
     2881 gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggc
     2941 cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
     3001 atcggccatg cagagttaaa ggtcgcaaga gaaagtaatg cgccaacaat tgcccctatg
     3061 ccgaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
     3121 agaatgaccg ccatcgcatt agatgacacc atcgacacgc agcccacaaa cgccgcaacg
     3181 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgccgcc
     3241 ataataaact gtatccacaa ccccgagcgg aacatattta acgcgccaat tcggcggacg
     3301 aagcggctgt taaagatggt catcacgaac aggaaaacaa tgtttagcgc aaagtaataa
     3361 ccaaagtttt ccggcgcgac gtggttaatt tcaatataga caaacggtcc ggcgctcagg
     3421 aacgagaaca tcccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta
     3481 tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgacgc
     3541 tccggtggta aggtttcttt gatcagaaag aagatcattg ccgaagccag aatcgccgcc
     3601 acggcgagga tccagaagat gtaatgccag ctcagccaca ccagtaccca gccgccaact
     3661 atcggtgcca tcagcggtgc aatggtcgtc accagcatga caaacgacat catccgcgag
     3721 aactcttctt tcgggtagat atcacgcatt aaagcgttga taaccacgct ggccgcagca
     3781 gccgccagtc cgtggaagaa acgcatcaca atcagctgct cgatagtttg tgccaacgca
     3841 cacgccaccg cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg
     3901 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
     3961 ctgagggtca tctgcgtact gcccgccggt acgccaaact gagctgaaat taccggtagc
     4021 gcaggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
     4081 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
     4141 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
     4201 tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc
     4261 acggcggcga acatacgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
     4321 tgcgttgggg taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc
     4381 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta cgggtgattc cagcgtcacc
     4441 agataggtct tctcgcaatg atggcgcggg gaagtaatgc ggtgcgacca ctgaccatca
     4501 tctgtcatta gtaccagacc ggtggtatcg atatccagac gccctgcggc gtgcagtttc
     4561 cacgctaccg gttcatccag aaaatagagc accgttgggt gatccgggtc gtccgtggag
     4621 caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
     4681 gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc attacggacg
     4741 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
     4801 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
     4861 acggaggtcg ggcattgacc cgacaatctg aactgttgtc tgcccagtat agcggtctaa
     4921 taacttccct caagggaaaa agatccgtgg catactatta gcagaataat ctacctacgc
     4981 gagaccatga tttttacact ccgcccatat cagcaagaag ctgtggatgc cacgctcaac
     5041 cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc
     5101 ctggtgatag cggaactggc gcggctggct cgtggtcgcg tgctggtgct ggcacatgtt
     5161 aaagaactgg tggcgcaaaa ccatgcaaaa tatcaggcac tgggactgga agccgatatt
     5221 tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg cagcgtgcag
     5281 tcggtcgccc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa
     5341 tgtcaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac tcacctgacc
     5401 aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg actgggcaaa
     5461 ggctggattt atcagttcca ttatcacggc atggtacgcg gggatgagaa agcccttttc
     5521 cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacgccg
     5581 ccagaacggc tggatatgcc ggtagtgcaa tacgatttca gccgcttgca ggcacagagt
     5641 aacgggcttt tcagcgaagc cgatctcaac cgcgagctga aaaaacagca acgtattacc
     5701 ccgcacatca tcagccagat tatggagttc gccgaaaagc gtaaaggagt gatgattttc
     5761 gccgccaccg ttgaacacgc caaggagata gtgggactgc tgcctgccga agatgctgca
     5821 ctgattactg gcgacacccc cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc
     5881 cagcgttttc gctatctggt caacgtcgcg gtactgacca ccggatttga cgccccgcac
     5941 gtcgatctta tcgccattct gcgccctacc gaatcagtga gtctttacca acaaattgtc
     6001 gggcgcggtc tgcgtctcgc tcctggcaag actgattgct taattcttga ttatgcgggt
     6061 aatcctcacg acctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtc
     6121 ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc
     6181 gctgacggga cattgattga acactttggt cgtcgctgtc agggatggtt tgaagatgac
     6241 gacggacatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac
     6301 gcagaaaatg atattgccgc ccgccgttgc cgcgagtgtg acaccattct ggttgacccg
     6361 gatgatatgt taaaagcggc gctacgactg aaagacgcgc tggtattacg ctgtagcggc
     6421 atgtctttgc agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat
     6481 gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca gcgtaccgcc
     6541 ttcgagcagc tttttatccg cccgcatacg cgcgcaccgg gcatccctct gcgctggatc
     6601 accgccgctg atatcctcgc ccagcaagca ttactgcgac acccggattt tgtcgtcgcc
     6661 cgcatgaaag gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt
     6721 cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag
     6781 tatagaatat cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc
     6841 ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa
     6901 agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat
     6961 ctacggtggc aaagaagcgc cgctggctgt cgaactggat cacgacaaag tcatgaacat
     7021 gcaagttaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat
     7081 caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc tgcacatcga
     7141 cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt
     7201 gtctctgcaa attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt
     7261 cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac gtccggcgct
     7321 gcaattgatc gcgcaaattc ggcggcgtgc ctttaatggt cagggtatca gttgctggat
     7381 cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag ccaccgccgc
     7441 taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca
     7501 gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg cctgccagct
     7561 ctttcgacag cgattccagt tcaatctctt cacctgcctg tagctgctca ttacagtagc
     7621 tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga
     7681 aatcatcgac cgcttgcaac agcccacggt tttgcgcttt cgcgtttaac ccttcgctgg
     7741 caccgaggaa atccataaag aaatctgcca ctttgcgccc tactcgccct ttcaggaaag
     7801 tgagatagcg ggtcgactct ggattggttt cccattcggt taaatcgata cgcgcaacaa
     7861 tatctgcatg attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacgc
     7921 gcatactgct caggttgctc aggactgcca ccagcagata ctccaccgcc agatagcgat
     7981 agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca
     8041 ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc
     8101 gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc
     8161 tatagacccg atgcagttct gccaccatct caacgacggt ttccgtcggt tccagcaatg
     8221 aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa
     8281 tctggttgat atccagactc atgataaact ctcctttaag accgggcggt attcaaccac
     8341 cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcgg cgtaacgaga
     8401 atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt
     8461 catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg
     8521 aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt
     8581 ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc
     8641 aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc cacgaagaca
     8701 aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa
     8761 aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg
     8821 ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc
     8881 atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg
     8941 ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc
     9001 gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt
     9061 ttccatctcc atcttaatcc catcgtctgg caactggtta tcaacccaga cgaaaatgag
     9121 atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg
     9181 gtgtttgcga cgtggagctg gcaaaagcta cgcagcctga cgcgtcgtcg gcgcttcgcg
     9241 cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt gtatatctgg
     9301 gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc tctttcgtac
     9361 ccgatgacgg cgcgacgttt tcttgagaag catggcctgc ttgatgcgca ggaatatcaa
     9421 cgccgtctta ttgagcaagg taatccagac gccgtttccg ttcagtatcc gttaagcgaa
     9481 ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggactg
     9541 aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt
     9601 tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc
     9661 tatggcatct cgccgagcta tatggacggc attctgtcga ctcgtacgcc tgcggcatta
     9721 attactgcgc ttaatcagca aggctatcag ctggggttat tctcatcaga tggctttacc
     9781 agcccgctgt atcgccaggc attgttgtca gatttctcga tgccaagcgt acgtacccaa
     9841 tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc acaagaagat
     9901 aaccgctggt tctcgtgggt ctctttcaat ggcactaaca ttgacgacag caatcagcag
     9961 gcatttgcac ggaaatatag ccgggcagca ggcaatgtcg acgaccagat caaccgcgtg
    10021 ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg tggtgattat cactgccggt
    10081 cggggtattc cgctgagcga agaggaagaa acctttgact ggtcccacgg tcatctgcag
    10141 gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc tctgactgat
    10201 cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc
    10261 gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttactgca
    10321 gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac
    10381 ggtaaatacc gtacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta
    10441 agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg attaattata
    10501 aatcagttag caaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca
    10561 cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt
    10621 tcaaatcctc tcgtgccgac caaaaatccc aaaaaaaacc aaccgtaaag gttggttttt
    10681 ttatatctgc atttaattcg ataaacagac cgcgacacat cacggcctgt ttattgtctg
    10741 ttatcagaac gaccagacca cacccgcctg agtattccac ggtgattcta cccctgcgcc
    10801 attgccatag ctgacagaca aatgaccgct taacgtcggg gtaaacgatg accttatccc
    10861 agcctgataa acgccacggg ttccagccac atcattatta aatttgccat cgtcattaac
    10921 tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc agggttccag
    10981 cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcctgctt cagcgcgtaa
    11041 tatgcgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt cctgaccatc
    11101 tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt caacccaacg
    11161 gaacccgctc tcgacatgag cacccgcgcc gttactactg taatcgccaa acgctgttgc
    11221 cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca ccaccccatc
    11281 aacataggca ccgttctgat gctcccaacc agcataagcc cccagggtat agctatcgat
    11341 attacctttg ccgccgcgat caaaaccaat atcagaatga gagtaaccaa agaacaagcc
    11401 gcgaattgtg ctgctttctt cacgggagaa acggctatcg ataccgagcg tcagcccggt
    11461 caatgtttgc tcaaagccag ctcccgcatc agtggtcacg ttgttgcggg tgttaattgc
    11521 cgaactccac atcgccgtat cgtaattaac gccttttacg ctaccaagac gctcacgcac
    11581 ggtgtccagt tctgcatcaa ataccagcgg ttgtgctgcc gccatattca gcacatcagt
    11641 ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt tgccattatc
    11701 cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc ccaacgtaaa
    11761 tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tcacctgctg ccgggctggc
    11821 accggtgtcc gtaacgaata ttttgaaatc acctgttgcc tgaccggtga cgttgagctg
    11881 atcgctctga tgattagcca tatcagtacg catataaaaa ttgccatttc ccgatagggt
    11941 attggtggtc aacgtagtga aattttccgc acttgccgtc acagatgaac gagtcactgg
    12001 ctcagccatc ataatcacat ttgcgccaga catcgcaagg ttggctaagt tgaggcgagt
    12061 gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt cgagcgaaac
    12121 atccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct cagaaaccac
    12181 taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga cctcaagaat
    12241 gccatcattc cctctgactg aaacgttaac catcgtgcca gcccagacat tagcgcggcc
    12301 attgttgata accatatttt cagagatggc tttagaggaa tacgttatac ttccattctg
    12361 atctgatcga ccgagatcat ataccgcatt tgcctgcact ctcgtaccag tatcctttcc
    12421 cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg tgtcctccat
    12481 aacaataagc ccggaaccat catccagctc ataatttttt gacacaccat cttttatact
    12541 aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga caattaattt
    12601 tccacctgct tttttatcaa cgccagttgc agtcccgcca tccataacct ccagtaagcc
    12661 gccactatcc acagtggtat tataagcgaa gtcattttct tcaactcgca aacttccgcc
    12721 gttttccaac agcatattat tagcaatacc atccttgata tcgaactgac cgagacggtt
    12781 tgttccgaat acctccatgg cccgggtggt tgttttaatc gcaccgcctg ctttctgatc
    12841 taccgcaaat gctaatcccc cctctttaac ctccagtaat cctccctttt cgatagcggc
    12901 aaagacggta atcccatctt tttcaataat taagcgcccg cctttaatcg taggttcatt
    12961 cgctgcgcca taaacctgta gcacaccatt attgagggta gtctggtttg ccgcaccacc
    13021 catttgaaca atttgataac catcatttaa tattgagtat tctgcggtgc cattcatttc
    13081 aatgtactga tacccgccgt taatttcagt attactactt ataccaaatt ctttaatatg
    13141 ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca gctggatatc
    13201 tttttccagg acaacaccat caactgtctc accatactca actgttgatg ccaatgctat
    13261 gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa gagaaacaga
    13321 accagattga tgcattgagc tttcatccta tgaaattaat tgctgttaaa agcattgggt
    13381 agagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt taaagaaatg
    13441 cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga tgcaaaattc
    13501 cgtctttata atgaaagtga tgccaaagcg aacgacaagg ttgtagtttt cgctacatgt
    13561 ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc tacgctcaaa
    13621 cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc cttttcaggt
    13681 gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac tggagcttga
    13741 tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg atctggcgaa
    13801 tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga gtggcctgga
    13861 taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc tgactgtatc
    13921 agatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt atctgttgaa
    13981 agatagcgac ccggaagtgt tgctggaagc gattcgtgcc ggagcgaaag gcagcaaagt
    14041 ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg cggaagaaga
    14101 tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg cacaggggct
    14161 gtcaaataaa cagattgcct cggtgttgaa tatttccgag cagacagtaa aagtacacat
    14221 tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca ttctgttcct
    14281 gcaacaacgc ggggcacaat aaaaaatagc ccgatggcat caccatcggg cctcttttta
    14341 tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg atactacgtt caatcaccgc
    14401 acggcgagta tcgttggcag gtaagagttt caacatcatc tcccacgcgg caacggcttc
    14461 gccaaatcgc tgctgctcaa aggcattaaa cgcgtacatg cttaacacac gaatattact
    14521 atgatccgtt ctcaccagct gacgtagcag ttcaccgccg aggcggttgt cgttgggatc
    14581 agatgaacgt gtcaacgctt cagcgtatcc cagtgcagca tcgctgttct tcggatcgag
    14641 gcgatacgca gtagcgtatg catcggtggc gatactggcg ttacccagcg ccatgccaac
    14701 gcggcccaac ataatccagc cttctatatc tcccggattt ttttgcagtt gagtacgcat
    14761 ccccagcgca aggcgcgaca tctcttcttc gttgagcgga tcggctttcg gatccagcgc
    14821 cctgtccagc aacgccggag cctgtgccgt ggcttgctgc cagattttca cctgctgata
    14881 attgccagtc tggtagtagc tgacgccagc cacaattaac gccaccacaa tacccggcag
    14941 ataaacaaca tatccggcac gcttaccttc cggcacgctt tgttcaggaa acgcttccgg
    15001 caccacgcgt acccgacgcc gcgagcgggc gtatatgacc caaccgccaa tgccaatagc
    15061 cactactgga agcacccaca gcagcacggt cagcggcgtt aacggcggat cgtaagtgac
    15121 gaagttgccg taacgcgcca ccatataatc gacaatctct ttcttacttt taccttcctg
    15181 catcagttca tacactttct ggcgcaggtc ggtggcaatc atcgagttgg aatcggcaat
    15241 gctgttgttc tggcatttcg ggcagcgtag ttcttcagtg agttgccgga actgctgctc
    15301 ctgcgcttca tccttaaact gcaacacgtc gatggtcgcc agcgctgagc cggagatcat
    15361 cagcatcagc acgcccaata aaaacctcat tgtgcggcct ccttactgta tttctcccac
    15421 agcggcttga tctcttcttc ccagacgcga ggattgagat cgcccgcatg gcgatagcga
    15481 atgatgccgt tgccgtcgat gaggaacgtt tctggcgcgc catagacacc gagatccagt
    15541 cctaacatgc cgtcgccatc aaacaggctt agtgcgtaag gattgcccag ctctttcagc
    15601 cagctgattg ccttctggcg atcgtcttta tagttcatgc cgaccacgcg gatgccctgc
    15661 gcagaaagct gattcagata ttgatgttcc gcacggcagg tcggacacca ggtcgcccag
    15721 acgttaagca gcaccggttt gccctgagtc agaacatccg cctgataaaa ctgccccgga
    15781 ttgtccagtg attcgaggcg aaacttcggc actggcttgc caatgagcgc cgattccaga
    15841 ttggtcggat catccccttc ggcattacgc gccagctgcc acagtagcgc cgcggcaatc
    15901 gccaggaaga taatcaacgg aattaacaat actttgcgct tcatacagcc tccggcgcag
    15961 ttttctgcgg gttcacgcgc ttacgatagc gaggatcaaa caggcacagc agcccgccca
    16021 atgccatcat cagcccgccc gcccaaatcc agcgaacaaa cggtttgtag taaagacgca
    16081 cggcccacgc gccgttttcc agctcttcac cgagcgcggc gtacaggtca cgcgtgatgc
    16141 cgccgtcaat cgccgcttcg gtcatcatcg acccggcagt gttgtaataa cgtttttccg
    16201 catacagcac cgtttccggc ttgccatcgc gcgttacgcc gatagtcgcc acaccgccac
    16261 gccagttcgg gccagtcacc tctttgacat cacggaaggt gaagcgatat tcatgaatat
    16321 cgacactatc gccggacttc atgcgcacat cacgctcaac gctatagttc tggctaaagg
    16381 caataccaac aattgtcact gccagcccca ggtgagccgc caccattccc caataactga
    16441 aggtggtttt cgtgccacgt gaaatacgca gcgcagcttc cgcaattgcc agcaccgcaa
    16501 tccagcaggc cattgccagg ccgagcaccg tcatcgccac aactttgctt tcgaacagcc
    16561 acggcagcag cagcgacagc accagcgttg agatgaaggc gataatcaat aaattgcgga
    16621 ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc gacaccaagc agcagcgcaa
    16681 acggcaccat cagccaggta aacatggtgt tgaagaacgg ttcgccaatc gaaatactgc
    16741 ccagtcccag ttgcttgtgc accagcggca gcagcgtccc cagtaatacc accagcatcg
    16801 cggcgaccag caaaacgttg ttcgctaaca gcagagattc ccgcgaccac agcgcattgt
    16861 ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag cagcagcgaa ccaccaatca
    16921 ccagcaccat aaaggcgagg ataaacatac cgcgcgccgg atcagacgca aacgcgtgta
    16981 ccgataccag cacgccggaa cgcacgagga aagtccccaa cagacacaac gagaaggcac
    17041 tgattgccag cagtaatgtc cacgctttga agctggcgcg ttgttcagtg accgccagtg
    17101 agtgcatcag cgcagtcccc accagccacg gcataaacga ggcgttttct accggatccc
    17161 agaaccacca gccgccccag ccgagttcgt aataggccca tgcggaaccg agcacgatgc
    17221 cgagcgtcag gaagatccac gctgccagtg tccacggacg agtaaaacgc gcataagtgc
    17281 tgtccagacg cccgctcaac aaagaagcaa tggcaaaagc aaacgccacc gagaagccga
    17341 cgtaccccat ataaagcaga ggcggatgga agatcagccc cggatcctgt aacagcgggt
    17401 taagatcgcg accttcaatc gggaagttcg gcaacgtgcg tgagaacggg ttagaggtaa
    17461 agagaatgaa cagcaaaaag ccgacgctga ccatccccat tatcgccagt acgcgggcca
    17521 caatatccag cggaatacgc tgactaaaaa tcgccaccgc aaaggtccag ccgctcatca
    17581 gcagcaccca cagcagaagc gaaccttcat gtgcgcccca ggtagccgcc acgcgatacc
    17641 acaccggaag ctgggtattg gagttgctgg caacataggt gacggtaaag tcgttgacca
    17701 cgaaggcatt gaccagtacc agaaatgcgc cagccacaga cataaacagc agccaggcaa
    17761 acaagcggga agacgccatc atgcgcgcat ctccgcgcgc cacgccccat agcggataca
    17821 cggacagcag cagcgcaatt cctagcgcca ggcacagcag cccgttacca atttccggca
    17881 tcatgatgct gggtccttat aaacactcgc cgggcgacgg tggttagctt ccatcgcttt
    17941 ctcaacttct ggcggcgtgt agttttcatc atgtttcgcc agcacttctt tcgcgaggat
    18001 atgattgcct ttttccagct cgccctgcac cacaacgccc tgtccttcac ggaacagatc
    18061 cggcaaaatg ccttcgtaag agacatctac tgaaccttca gcgtcgtaaa tggtgaaagt
    18121 tacttttagc gaattgggat cgcgctgcac actccccggc atcaccatcc cgccaacgcg
    18181 cagacgctga ccgacttccg gcatctgctg agtttcacgc ttgccgtaga gaatttcccc
    18241 cggcgtataa aagagatcga tattcgagcg cagcgcatac agcaccagac cgatagtaag
    18301 cgccagcccc gccaaaacgg cacaggcaat ccacaagcgg tttttacggc gaatattcat
    18361 gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac gcgcccgctg ttgcgccacg
    18421 ccacgcagaa ttgcgcgatg ctgcatcacc gagtgcacga ctaaaatcac cagcggaata
    18481 acggtcatcg ctaccgccag ccagacaaaa aaggcgtaac cgcccattgc gaaaaattca
    18541 ttccaggaag caaatgcagg ggtcatttac ggcctctttt cagtatcagt tcactcaccc
    18601 acggacggcg tttttccatc agcaaaatca aattacgcat ccgcatcagc gtcagcgtgg
    18661 cagacagcag caggaagcca aaaatcgacc agcgcagcgg cgaacgcatc gccggatcga
    18721 tactttgctg catccgcgtt gatccctgat gcagggtgtt ccaccactcc acggagtaat
    18781 gaataatcgg cagattcacc acgccaatca gcaccaggat acctgccgca cggcccgcca
    18841 gacggcggtc gtcgaaggcg tgccacaggg caatcacacc cacatacaaa aacagcagca
    18901 ccagttcaga agtcagacgt gcatcccata cccaccaggt gccccacatc ggttttcccc
    18961 atgcagagcc ggtaaccagg gcaataaagg taaacacggc accaatgggg gccatcgccg
    19021 ccaccgccag gttggccatt ttcatctgcc agacaaggcc aataaacgcc gccaccgcca
    19081 ttgatgcata aatgcccatc gaccagatcg ccgcaggcac atgcaggtag ataatgcgat
    19141 agctatttcc ctgttgataa tcagccggag caaagccgaa tccccagatc cagccgacgg
    19201 taagcacgac cacactggca attgccagcc acggtataaa ccagccacag atttgataca
    19261 gccgtggtgg gatcgccagt tgatgcagtg ttttccacat agtttcgata ccagactcga
    19321 acaaaaatca gtaatccagc gttattgaat gctgattcgt aacgctgccg ccgtcgcaaa
    19381 aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa attgccagat acccgtcaac
    19441 gggcaaatgc atggaagccg cgtccatcgc ggcggtggca aagatgagta atgggatagt
    19501 cagcggtaac accagtatgc tgagcagcac accaccgcgc ttaagtccca ctgtcagcgc
    19561 cacgcccggt gcaccgagaa agccaagcgt aggcgttccc agcagcagcg tcagcgccat
    19621 cacttgccag ccataaacat ccattcccag tagcattgct accagtggcg aaaggatgag
    19681 taacggcaga ccggttacca tccagtgcgc catcaccttc gccagcacaa cggcgggcaa
    19741 gggtaacggc aacaacatca attgttcaag actgccgtcc tgcaaatcgt cacggaacag
    19801 acgctccagt gccagcaagg atgaaagcag cgcagcaacc cagataacgc ccggtgcaat
    19861 acgcgccagc agttgcggct ccggaccgat actgagcgga aaaagggtaa tgacaatcag
    19921 gaagaaccac agcgggttgg cgatttcggc gctgtggcga aacgctacgc gcagctcaag
    19981 acggaaaatg cgccagaaca tcatgcggcc ctcgtttgcg tcagtgaaat gcggcgaatt
    20041 ttactttcag caacgttgag cggctggtgg gtagtcagaa tcacaatccc cccctgctcc
    20101 gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt taacgtcaat cgcggtaaaa
    20161 ggctcgtcga ggatccataa cgtggcacgg gtcagccaca gacgcgctaa agcgacgcgg
    20221 cgttgttgcc cggcagaaag ttgatttacc ggaatatctt cgaatccggc aagcccagcc
    20281 tgcgccaggg cttccagaca ttgtgcggta tcgccatcgc gatgataaaa gtgcagattt
    20341 tctaacgccg tcagccgggt tttgatcccc ggctgatggc ctatccataa caggttttga
    20401 tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc agagaacgtc acccgcatca
    20461 gggcgagaca accccgtcag caaacggaga agcgttgtct tccccgcgcc gttgctaccg
    20521 gtgatttgta cccactctcc tgcgttcagc gtaaatgaca agccactaaa taaggttcgt
    20581 tcatcccgct cgcaaagtaa ctctctggct tcaagcatac ccacgcaata accctgttaa
    20641 aaacctggct cgacttcgcg catatcgggc agcttgtgcg ctatcccttt atggcaatca
    20701 atacaggttt gcccatcttt caccgcctgg tcatgcatct tcgcggcaac cgatttctgg
    20761 gcggttgtat ccatatactc gaagttgtga cagttacggc actcctgcga gttattgtcc
    20821 ttcatgcgcc gccactcatt ctgtgccatc gtcaggcgat gagcttcaaa tttctgcggc
    20881 gtgtcaataa cgccaaaaat tttaccatac agctctttac ttgctttgag cttgcgtatc
    20941 atcttcggca caaactcgtg cggaacgtga caatccggac aggtcgcacg aacgccgcta
    21001 cggttgttgt agtgcacgga atccatgtat tcctgataca ccgtgttacg catttcgtgg
    21061 cagctaatgc agaactcttc ggtattggct ttttccatcc cggtgttaaa gccgccccag
    21121 aagacgatgc cgccaacaaa gccgatcaac agcagcgtcc ccagcgccag acggctgggg
    21181 gtacgccacc atttccacag gcgcttaatc agaccaggct tacggtcaga atttcccata
    21241 ataacctctt atttcccgta accttttgat ggggtaaagg tattccccac gattggcgcg
    21301 gtatcggcct gcggtacgtg acattgcaga cagaaataac gacgcggagc cacttccgcg
    21361 cccactttgc cgtcgctgtc cataaagtga gtaggactga tgcgcggcgc gccagtggtg
    21421 cgatagcttt cgacaccgtg gcactgcaag cagcggttgg tattggtcgt tacctgataa
    21481 ccttcaacgc tatgcgggat cattggcggc tgattcacat agttcagcgg catccgatcc
    21541 tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg atacttccgg cgactggcta
    21601 aaatcgacgc cattagccgc ccaaaccgcg ccgctcacca ccagggccag catcgccgtc
    21661 cattgacaca gcgctttctt caggtcatgg cttttcatga tttcgctccc gaactccatc
    21721 gtgtagttat tgtaaataca tcctcagaac agacatccac gcagcgaccg cacgtcatgc
    21781 aatcgcggct ggtgacctgc accgggcttt gttcatccag caccggggca cgtagcacat
    21841 gcggttccgg gcaaacatga aaacaatcca tacagcggtt acatttctgc cgatcggtgg
    21901 ccgcaacggt aatgacacct ttgctaccca gcacgccata cagcgcgcca accggacaaa
    21961 tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa taaaaacagc gcgagaataa
    22021 gcagcgcgcc gctgccgaat cccattacca gactgcgccc catcagggaa acggggttga
    22081 tccattccca gatgagtgtg ccggttaagg ccgatcccag cagaatgacc accaacagca
    22141 cgtagcgaat gtggcgaggg atcgtcgcag actgattgag gtcaaagcgc ctgcgtaacc
    22201 agttcgctaa gtcggtaatc gggttcagcg ggcagaccca gctgcaaaat aatcgctttg
    22261 ctgccagggc gtagagcacg gtgataatta ccgcaccggt caacgccacc gtggcaggca
    22321 gatgaccgct ggcaagactt tgcagcgtca tcagcggatc ggtcagcgga atggtgtcga
    22381 ataacaggct actgctgtag ttgccgtgca agatccacac accaaaccac ggaccgctca
    22441 aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa caccagccaa cggtggctgc
    22501 gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc acgtttacga tttgccattg
    22561 ttcccctcca gccagccgaa gcggtaatgg tgacctaact cccctttcgc cagtgacagc
    22621 ggtaacacct tgattgccgg ttgttccagc acgcacactt tttcgcactt accgcagccg
    22681 gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt gcttaccggt acgcgtgttg
    22741 cgctccagct ccagggtgat tgcctcatca attttcgggc attcgcgata acaaacatcg
    22801 cagcgcagcc cctgaaagtt gagacagttt tcctggtcca ccagtaccgc cagccccatc
    22861 cgcgcgtcgt cgatcgattc gatctcacga tccagcgcac cgctcgggca cactttggcg
    22921 cacggaatgt cctcacacat ttcgcaagga atatcccgtg cgacaaaata tggcgtgccc
    22981 gccgacaggc cagaagccag cgtcgccagt tttaaggtgt cgtaagggca agcctgaaca
    23041 cactgaccgc aacgcacaca ggcactggca aaggcgttct cgtttatggc tccgggcggg
    23101 cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta accccagcgc cacacccacg
    23161 gcagccagcc cgccagctgt gcgaacgaca tcgcgcagaa agcggcggcg accattttga
    23221 ggtttcgctg accgggacat aatggcttac accttctcca gtttgaccgc gcacttcttg
    23281 aagtccgtct ctttcgagag cggatcggtc gcatccagcg tcagtttgtt aaccagctgt
    23341 gcggcgtcga agaacggcat gtacaccagg ccctgcggcg gacggttacg accgcgcgtt
    23401 tcgacaatcg agatcacttc gccacggcga gaaaccactt tcactttgtc gccacggcgc
    23461 agatcgcgcg ctttcgcatc cagcgggtga ataaacagga ccgcttccgg gaaggcacgg
    23521 tgcagttccg gtacacggcg agtcatactg ccggtatgcc agtgctccag gacgcgtccg
    23581 gtagagagcc acaggtcgta ctcttcatcc ggtgcctccg ccgccggttc gaacggcagc
    23641 gcgaagatca ccgctttgcc atccggttta ccgtagaact tgtagccttc gcccgctttc
    23701 acgtatgggt cgttaccttc gctgtaacgc cactgcgttt ctttaccgtt cactaccggc
    23761 cagcgcagac cgcgtgcttt gtggtaatca tcgaacggtg ccagatcgtg accgtgacca
    23821 cgaccaaacc atgcgtactc ttcgaacagc cctttttgca gatagaagcc cagctcgcgg
    23881 gattcatcgt tcagctgatc ttccgccagt tctgataccg ggaatttgct cacttccggg
    23941 gtggcataca gaacttcgta cagcgttttg ccacgcagtt ccggtttctt cgccagcagc
    24001 tcttccggcc atacttcttc agttttgaag cggcgggaga actggactaa ctgccagaga
    24061 tccgatttcg cttcgcccgg tgcctgtacc tgctgacgcc agaattgagt acggcgttcg
    24121 gcgttaccgt aagccccctc tttctctacc cacattgcgg tcggcagaat caagtccgct
    24181 gccagcgcac tgactgtcgg atacggatcg gagacgatga tgaagttgcg cggatcgcgc
    24241 cagcccggca tacgctcttc attgatgttc ggcccggcct gcatgttgtt ggtacacatg
    24301 gtccagtaaa cattcagctt gccgtctttc agtgcacggt cttgtgctac cgcatgcaga
    24361 ccgatttttg ccggaatggt gccgctcggg atattccact tcttctcgca gatatcacga
    24421 tgtttctcgt tagtcaccac catgtccgca ggcagacggt gagcaaaggt gcccacttca
    24481 cgcgcagtac cacacgcgga aggctgcccg gtcagggaga acggaccgca acccggctgg
    24541 gaaattttgc cggtcagcag gtgcaggttg tagaccaggt tgttggccca cacgccacga
    24601 gtatgctggt tgaagcccat cgtccagtag gagatgactt tcttgttcgg atcggcatac
    24661 agctgcgcca gttgttccag ctgatctttt ggtacgccag tcatttcggc ggttttttcc
    24721 agcgtatact cggcaacgaa ggctttgtaa tcttcaaagc tcatcggttc ggaggcgtca
    24781 gaacctggat tcttcgctgc tttttccagc ggatgggtcg gacgtaaacc gtagccgatg
    24841 tccgtcgccc ctttgcgcag gttaacgtgt ttgctgaaga agtcctgatt tatcgcattg
    24901 ttttgaatga tatagttggc gatatagttc aggatcacca ggtcagattg cggcgtaaag
    24961 atgatgccgt tatctgccag ctcgaagcta cgatgctggt aggtagaaag caccgccacg
    25021 gtaacgttct ggttagagag acgacggtta gtgatgcgcg accagaggat cgggtgcatc
    25081 tccgccatgt ttgcgcccca cagcacaaac gcgtcagcct gctcgatgtc gtcatagcag
    25141 cccatcggct catccatacc aaaggtacgc ataaagccaa ctaccgccga cgccatacag
    25201 tgacgcgcgt tcgggtcgat gttgttcgaa cggaagcccg ctttgaacag cttggacgcg
    25261 gcataacctt cccagatagt ccactgacca gaaccgaaca taccgatcga ttccggccct
    25321 ttttctttca gggcggtttt gaatttctct tccatcacat cgaaggcctg atcccaggtg
    25381 attggggtaa attcgccttc tttgtcatat ttaccgtttt tcatacgcag aagcggctgc
    25441 gtcaaacggt ctttaccgta catgattttg ggcaggaaat agcccttaat gcagttcagg
    25501 ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca ccacacgccc ctgctgcgtt
    25561 ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg gcgctttatc ccatttaatg
    25621 gcttcctgct gaccaacaac ggcgcgggca acgcccggca cgctgagacc ggcagccgcc
    25681 gcagcggccg caacggcgtt agctttcata aagctacgac gactgagttt catggtgttt
    25741 cctcaccttg ctcttcctgc tggtgataaa ccagcgacac cgccagcacg ccctctacgt
    25801 tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc ttctgcttcc accaccacaa
    25861 tcaactgacc gctcggcgcg tcgctgacag caacttcaca gccgggaaag gcgttcagtt
    25921 gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac gaccaggctg caaacttgcc
    25981 agttagtgtg catggagata ctccgcagtt atggctgata ccggacagct ggctgcacat
    26041 gctccacatc cgttgcaaag ttggctatta agttgcggct ggtagatccc ggagagcgtc
    26101 gggcgaaaga taattgccat tggctcacag ctatcctgac agcggcggca ttcaactgac
    26161 tgatacgcca ggcacgcgtc cccaatggtg aactgcaaat cccaggccct ggtgtggcgc
    26221 ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt aacagaagct gcactcgtta
    26281 tttttgaaat taacgctcgg atagccaccc gcgccgcgtt gcagaatgtt gttttcacaa
    26341 gcattaatac aggcgtcgca gcgggtgcaa tgggtcagaa aatgagattc atcaccggac
    26401 cagggcggac ggataccgtt actggctttg cgccagcgac cagtgagtat gccccgacgg
    26461 gatgcatcaa tcttcacatt gaccttccat cattaacgcg ctctgaaaat tgagagcgac
    26521 caaataaacc gcataattaa taagccattt
//
LOCUS       DARNVM010000056        26442 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.056, whole genome shotgun sequence.
ACCESSION   DARNVM010000056 DARNVM010000000
VERSION     DARNVM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.056"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            174..965
                     /locus_tag="SI621_002090"
     CDS             174..965
                     /locus_tag="SI621_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064813.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVINTLLNATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTRSVLEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRYIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPPDETPVKSAVGNAVILLAKDVIYNEYFFDLRKSFFVSVKDLMASGTKGIPETQKYP
                     DVYCWIPLFSINYGVVITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1371..1556
                     /locus_tag="SI621_002091"
     CDS             1371..1556
                     /locus_tag="SI621_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEI4064814.1"
                     /translation="MDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKLGRSSRWL
                     KSEVEAWLQARIAQSRP"
     gene            1644..2252
                     /locus_tag="SI621_002092"
     CDS             1644..2252
                     /locus_tag="SI621_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064815.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHN
                     LTPGWVSFNGEKPEIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLIWLCWLDLMTGNSLDDWTENLK
                     RKSEKEMGEWIIDRQKQNAALADLMDQYVLLAYRTTVDDNRN"
     gene            3961..4176
                     /locus_tag="SI621_002093"
     CDS             3961..4176
                     /locus_tag="SI621_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064816.1"
                     /translation="MDELKSAREQQKRRDERADGSVPLKLSGKIHKQPESIARSRGAP
                     PPKKLLNEMIEQTYQDFVANEQHKTWS"
     gene            complement(4630..5823)
                     /locus_tag="SI621_002094"
     CDS             complement(4630..5823)
                     /locus_tag="SI621_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4064817.1"
                     /translation="MSTVSLKQLYGKTHAYWSGVFAMTLCVFVLIASEFMPVSLLTLI
                     AHDLHVTEGLAGQGIAVSGALAVLTSLTISRIVGDLNRKYLLLGLTVLMAISGIIITI
                     APDYPVYMLGRALIGVVIGGFWSMSAATAIRLVPQHQVSRALAIFNGGNALATVVAAP
                     LGSYLGTTIGWCGAFLCLVPLAIAAFIWQWVSLPPMESDKTQKPQRSVLRLFSVAIVP
                     TGLLACGLFFMGQFALFTYVRLFLESVTRVGPSDLSLILLAIGVAGFVGTMIVSTFLN
                     TIFYKTLIMIPLLMAAITGALLLVGHHIWAVAVLLSLWGLLATAAPTGWWTWIARALP
                     EDAETGGGLMVAVIQLSIALGSTAGGLVFDSLGWRSAFGLSGLLLLGAVAMTCATSRQ
                     NRKAQ"
     gene            complement(6001..7137)
                     /locus_tag="SI621_002095"
     CDS             complement(6001..7137)
                     /locus_tag="SI621_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4064818.1"
                     /translation="MTAFTQKLTAAIPTMLLCVSLSGVTNMSYADTTNPNAPVSQVEK
                     WDKTFAESTKVDHRKVTFPNRYGITLVGDLYIPKDRGDRKLAAIAVSGPFGAVKEQSS
                     GLYAQTLAERGFVTLAFDPSYTGESGGYPRNVASPDINTEDFSAAVDFLGLQKEVDRN
                     RIGLLGICGWGGMALNDAAMDTRVKAVASSVMYDMSRAMGHGVGDGKDRYTTADRRAV
                     LQYLNMQRWKDAENGAFAPGGHDIYVDEKGNVTVSDRILPETLPANSNPVLKEFFDYY
                     RMPRGFHARSVNSTSAWNATMPLSFMNMPLLSYANEVTIPTLIVTGEKAHSRYFAEDA
                     FKAIGSKEKELVIVPEANHVDLYDNVAGKIPFAKFEQFFKANLR"
     gene            7308..8192
                     /locus_tag="SI621_002096"
     CDS             7308..8192
                     /locus_tag="SI621_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4064819.1"
                     /translation="MAKRENYNDLYLFMQVVREGSFTAAAQRLGLAQSGISRAVRELE
                     ERLGVQLLVRTTRKLSLTHAGELLYRNVESGFDALDMGLATLAHYRHTPSGTVRINAS
                     QHAIDKVLLPKLAVFKQRYPDIRLELISESRFVDIITERFDAGIRLGSEVDSGMVAVR
                     ISPDMEMAVVGTPGHFQRYGFPQTPTDLVGHPCIAYQFGDGSLYAWELNQDGKKITHQ
                     PQGQWAFADSYMEAKAARLGLGLAYVPEELVSDDIEQGALIRVLQCYSQRLEGSYIYY
                     PHRNVSPALRAVIDTLRI"
     gene            8422..9294
                     /locus_tag="SI621_002097"
     CDS             8422..9294
                     /locus_tag="SI621_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEI4064820.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQVQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            9666..12512
                     /gene="ag43"
                     /locus_tag="SI621_002098"
     CDS             9666..12512
                     /gene="ag43"
                     /locus_tag="SI621_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HEI4064821.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVSGGTLENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLTQGGALVTSTAATVTGSNRLGNFTVE
                     NGKTDGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGYRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            12583..12816
                     /locus_tag="SI621_002099"
     CDS             12583..12816
                     /locus_tag="SI621_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HEI4064822.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            12940..13422
                     /locus_tag="SI621_002100"
                     /pseudo
     CDS             12940..13422
                     /locus_tag="SI621_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            13514..13999
                     /locus_tag="SI621_002101"
     CDS             13514..13999
                     /locus_tag="SI621_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HEI4064823.1"
                     /translation="MTTVTHNSTTPSVSVTTASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            14015..14491
                     /locus_tag="SI621_002102"
     CDS             14015..14491
                     /locus_tag="SI621_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HEI4064824.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            14554..14775
                     /locus_tag="SI621_002103"
     CDS             14554..14775
                     /locus_tag="SI621_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HEI4064825.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            14938..15312
                     /locus_tag="SI621_002104"
     CDS             14938..15312
                     /locus_tag="SI621_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HEI4064826.1"
                     /translation="MSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPAPATTS"
     gene            15359..15733
                     /locus_tag="SI621_002105"
     CDS             15359..15733
                     /locus_tag="SI621_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HEI4064827.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLARLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYELVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            15730..16236
                     /locus_tag="SI621_002106"
     CDS             15730..16236
                     /locus_tag="SI621_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064828.1"
                     /translation="MMKLALTPEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTSDVGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPT
                     FNWYPGTT"
     gene            16233..16430
                     /locus_tag="SI621_002107"
     CDS             16233..16430
                     /locus_tag="SI621_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064829.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCEPGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRHLQLQHQNR"
     gene            16515..17357
                     /locus_tag="SI621_002108"
                     /pseudo
     CDS             16515..17357
                     /locus_tag="SI621_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            17699..17869
                     /locus_tag="SI621_002109"
                     /pseudo
     CDS             17699..17869
                     /locus_tag="SI621_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18680..19663
                     /locus_tag="SI621_002110"
     CDS             18680..19663
                     /locus_tag="SI621_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEI4064830.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELYMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPSVHLDAPFKTVIQRITSGCQGMVMVEDAEGELAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEETMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            19735..20883
                     /locus_tag="SI621_002111"
     CDS             19735..20883
                     /locus_tag="SI621_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HEI4064831.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            20907..22583
                     /locus_tag="SI621_002112"
     CDS             20907..22583
                     /locus_tag="SI621_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HEI4064832.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQIDPKGNIFLPNVGPVKVAGVSNSQLNTLVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            22593..23333
                     /gene="kdsB"
                     /locus_tag="SI621_002113"
     CDS             22593..23333
                     /gene="kdsB"
                     /locus_tag="SI621_002113"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEI4064833.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTRQDALYFSRSPIL
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            23330..25357
                     /locus_tag="SI621_002114"
     CDS             23330..25357
                     /locus_tag="SI621_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEI4064834.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQTEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSDIFTVLQWLQLQRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLINHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQRANEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTLADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPIVIRKNNIDRVINIYRKLIMFCRVRLG"
     gene            25392..>26442
                     /locus_tag="SI621_002115"
     CDS             25392..>26442
                     /locus_tag="SI621_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEI4064835.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRNRQYQAYNQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWNEARCWVRAYWRKQLYRVTQRNVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKKYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNSLYDIVDL"
BASE COUNT         6827 a         6642 c         6744 g         6229 t
ORIGIN      
        1 atgcttcatt ttcggtggtt ctgtgtgata gatttatgct gactacagtg gcagtaatac
       61 tgctttccac cacacgagga taaaaccagt caaaaaatca attatatgaa ttaacagtat
      121 tttttatgaa atgttatgca tatcaaccag tatgattaac agaaaacttc atcatgacaa
      181 cacgtataac tttcaatatg accagtgatg agacactgcg aattgtggat gagtactgtc
      241 atacccacaa actttcgcgc tctaaggtaa tcaacacatt actcaacgcc acagcacctg
      301 tccttaatga cattaactgt tactatcaac ttgcaggcga actgcagtca cgcttattaa
      361 atggagtgta tcaacgggat cttccccaca aacgcaatgt cgtcagtgca gagaagtatt
      421 gtctggagat ctgggaaaac aaacttttca ccagaagtgt cctggagttt gacagttcaa
      481 atggtgttct gtacgcgctg aaacataaaa gacactatcg ccgggataag atgattggaa
      541 gggttgaaag tcggtatatc aaagacattt gcgaatacca gatgcaacta tccggggaga
      601 agacgaaata tgcctgtttt atttatatag aaagaacaat atacaatcac gataatccac
      661 ctgatgaaac acctgtaaaa tcggctgttg gtaatgcagt catcctgctg gcaaaggatg
      721 tgatatataa cgagtacttt tttgatctgc ggaagtcatt tttcgtgagc gttaaagatc
      781 tgatggcctc tggcacaaaa ggtattccgg agacacagaa atatcctgac gtttattgct
      841 ggatcccgct tttctccata aattacggcg tggtcattac cccggtctat aaaattgatc
      901 ccctgaaacc cgtaacggta aaaaaaccgg ataagatcac tgttgtttgt aattacagag
      961 aataatctca gtcttctccg ctcaccacaa cagtgtttac ctcgctttat cttccgttca
     1021 gtcaatacaa accaccgctc tggcaggtac agggcgtggt ttctctccgg tactcccctg
     1081 cgcagtaata atcatcactg aaaatactga ttccgtcacg acaaaatacc atcccatatt
     1141 ccggcaacac aaaatcatct tccgtttata ccggcttcac tgacaaaaca gacaacctgt
     1201 attgcctgta cacaaccgtt tctctcctgg caggaatatt cccctactga ttttttacgt
     1261 ttctccccgg ttgttatgcc ggtgaaggtg gtgcgttgtt ttcatcatca acgccggttg
     1321 agattaacaa catccggagg agatttttat gacccccccc agtttcgctg atggatgacc
     1381 agatggtcga tatggcgttt atcactcagc tgaccggcct gaccgataag tggttttaca
     1441 agctcatcaa ggatggggcc tttccggctc ccatcaaact tggccgcagc tcccgctggc
     1501 tgaaaagtga agtggaagcc tggctgcagg cgcgtattgc acagtcccgt ccgtaatttt
     1561 taaccactgc cccttcaccc acagcagacg caatctccgg cctgccggcg gcgttctgct
     1621 gcctgttatc ccccgtgagg aatatgaaaa tgaaaccaca gtatcagacc cgttacgagc
     1681 tgctccacga aagctaccag aaatggctga ccggcttcac ccggcacgcc gtatcctggg
     1741 gcgtgtgtca tccgaatatc cactacttcc ataatctgac gcccgggtgg gtgtcattca
     1801 atggtgaaaa accggagatt gccatcgtcc cccggagtct gcaccggctg atttatggct
     1861 ctgacaaacg ggccacgccg cccctggatg atgatctggt ggtgaactta tgcaccagcg
     1921 aacatctgct ggttcatcat ccgatgctgg aaggcattct gctgtctgaa tgtgaacgtc
     1981 tgaggcagcg ttctctggca aataaactga tcagcctctt ccgtcagttt ggtggcactg
     2041 agttgcgcct caaactgatc tggctttgct ggcttgattt aatgaccgga aacagccttg
     2101 acgactggac ggagaacctg aaacgtaaat cagaaaaaga gatgggggaa tggatcatcg
     2161 accgtcagaa gcagaacgca gcactggcgg atctgatgga tcagtacgtg ctcctggcat
     2221 accgcacaac ggttgacgat aaccgtaact gatgccggtt gcttccggaa ggtgcggcaa
     2281 aatacgggaa tgctgctccc ggccccgcat gctcacctgc agagcggggg cgtcgtcata
     2341 ctgagccatg acggatgtct taagggcatc ctgtcactgc tgagctggca tgctgcggta
     2401 catgcctctc ctggtgacaa taagtacatg gcgacagcgc tccctgaacc gcggaagaga
     2461 tgtacacggc cggacgccgt cagtgtatac gggtagacgt tccatgctgc atgactacat
     2521 ccggcccgga tatcaatgag tccgtcagga acgcttcact tcgaaacccg gcctggatac
     2581 cagcgccggc atgccgggaa gaagttgata tggtggttgc agggcaacag caatcctgaa
     2641 tcaactgctg catcactcaa ccacgctgaa tatcaaagga gagcgtgacc agttaaaaga
     2701 gaaacgcaaa tctgtagtgc tgcacaaaaa tacaacatca gtcagtgagg atgaaatggt
     2761 gaaaagcaga cagcatcaat attgaaaata tcctggctgg catgaaaatg gcccggcccg
     2821 tggcgttgac atattctctc ctgagcatcc tgaatgactg cagttactgt ggaaaagtaa
     2881 gcagtctcca cccacggcac cacttaacag acattcagta actccccccg gactttctat
     2941 gatgcattat cggtgcactg aatacaggag agtgcgctta aaaccgcaga ccgcattgcg
     3001 ggtgttactg catgactgcc tggcggtatt gtgagatgtc ggcaggaacc gatgtctgcg
     3061 cagggggaac ttccgtgatc gggataagtg cagaagaaat cagatcacga cgtaaacctg
     3121 cagtgcctga cgctgcaagt ggcattaacc atgggacgtt gccgatcccg gattgcggaa
     3181 acaggagcac tattttctgc cgcggggagt acctctggca gtaaatccgg ctcgctcatc
     3241 tgaccgtctc tctcccggaa aagaccagaa atgagatcgt cacctcaaca gcagggaaga
     3301 tgtcttgaca gactggttat attgcatacg atttatgaag aaaaagacag caaatatgga
     3361 ggctatagcg gagaacaccc tacgaaattc ctgtgaaaaa cgattgaaaa aacgatcaga
     3421 ttcgattcgt ttttatatgc ttttgggcac gtggcgatga cgaactgttg catgtgcaga
     3481 ggagaatgca cccggaagca ccagaatcat ccagaaaggc agaacgatta cgcatcaaac
     3541 caggagacag caatatcagg ataacatact gatttaaaat gaatttacag caatacgtga
     3601 aaacattttt tacttaaagg agacagatgc gctgaagaca gtgcaggtat ttttatataa
     3661 aaatgagaag gttacgggat gtctatcagg cacactatga ccggagaata tcagtcaggc
     3721 ttaaaaagac aataaataca gtgagttaaa tacacagaga aaacgaaaac gtagtatcag
     3781 agggtacatc ttacaacaga gactgaattt ccggtttttc ataaccggca cattccggaa
     3841 agagcaggct aaccggaggt tttctgacac cagtccctgg cagtttaatc tgtacatcat
     3901 gtacggatgt tttgttcgca tttttaaaat cggcaaaaat gcgagaagag cagctatatt
     3961 gtggatgaac tgaaaagtgc cagggagcaa caaaaaaggc gtgatgaacg tgctgatggt
     4021 tccgtcccgc tgaaactgtc cggaaaaatc cacaaacagc ctgaaagtat tgcccgaagt
     4081 cgtggagccc cccccccaaa aaaactgctg aatgaaatga ttgagcagac gtaccaggat
     4141 tttgtggcaa atgagcagca caagacatgg agttaaagac tgcagggccc cggttaccaa
     4201 ttctgcagca tacacaacga atgtatccgg ctcttacctg tgaggggcac gaaagttata
     4261 cattgagcat gagtggataa tctcgggtga gagtctcccg tttttacact attcactaaa
     4321 ggtgaggcat atcattcagt ctctctgaat tcttcatttc agtaacttca gctaccttcg
     4381 tttcatctcc cccacgaacc ggggccgcaa cttgttgtat caatatcaaa ttgttggtca
     4441 ttactgtcct gtatcactgg gctggcaagt gaactgaaat ccgcacctta atattctgac
     4501 tggctgcgta cattcatggc atgaagcggc cggagcggct gagctgaagg tccgttgtga
     4561 gcgaggagtg atcattagcc tctcagccgg atgccttaaa gagcgtacgc cagaaataaa
     4621 aggattgcgc tactgcgcct tcctgttttg acgggatgtg gcacatgtca tcgctaccgc
     4681 tcccagaagt aacaaaccac ttaaaccaaa agcacttctc catcccagac tgtcgaatac
     4741 cagacctcca gccgttgaac caagtgcaat agataactga atcactgcaa ccatgaggcc
     4801 tccccccgtc tctgcatctt cgggtaacgc gcgtgctatc catgtccacc accccgttgg
     4861 cgcagctgtc gcaagtagcc cccacaggct gagtaaaacg gcaacagccc agatatggtg
     4921 tccgaccagc agtaacgcac cggtaatcgc tgccataagc aacgggatca ttattaaggt
     4981 cttataaaat atggtgttca ggaatgttga gacgatcatt gtgccgacaa agcctgctac
     5041 acctattgcg agcaaaatca gagaaagatc agaaggtccc actctggtga cgctctccag
     5101 aaagaggcgc acgtaggtaa ataaagcaaa ctgtcccata aagaagagtc cgcaggccag
     5161 cagtcccgtt ggtacgatgg caacgctgaa caggcgaagc acagatctct gtggcttttg
     5221 tgttttatca ctttccatcg gaggcagact gacccattgc cagatgaacg cagctatcgc
     5281 aagggggacc agacataaaa aggccccaca ccagccgatg gttgtcccca gatagctacc
     5341 aagtggcgct gccacaacgg tggccagtgc gtttccgccg ttaaaaattg ccagtgcgcg
     5401 ggaaacctgg tgctgcggca cgagccgaat ggcggttgca gccgacattg accagaaccc
     5461 tccaatgaca acaccaatca gagccctccc cagcatataa accggatagt caggtgctat
     5521 agtgataatt attcccgata tggccattaa gaccgtcagt cccagcaaaa gatattttcg
     5581 attcaggtct ccgacaatac gggagatagt cagactggta agcaccgcca gcgcaccaga
     5641 gacagcaatt ccttgccctg ccagcccttc ggtaacgtgc aaatcatggg caatgagcgt
     5701 aagcaggctg actggcataa actcagaagc gatcagcaca aacacgcaaa gcgtcatggc
     5761 gaatacgccg ctccagtatg catgcgtttt tccgtataat tgtttgagac ttacagtgga
     5821 cattattagc cccccttaat atcgaaccag ccagattcaa gcgcaatcgg gtcagaagtt
     5881 attcagggaa taacgacata atagttgggt gagaatatag ggcgatgcgg catattctgc
     5941 atcacaccag gcagttaaaa gccacccgtt tacgacaggt ggcctggtaa acctttcggg
     6001 ttatcttaag tttgccttga aaaattgctc aaatttggca aatgggatct tacccgcaac
     6061 gttgtcgtac agatcaacat ggtttgcctc ggggacaatc actaactctt tctctttgct
     6121 gccgattgcc ttaaaggcat cttcagcaaa atagcgtgaa tgggcttttt caccggtcac
     6181 gatgagtgta gggatagtaa cttcattagc gtaactcagc agcggcatat tcataaatga
     6241 cagaggcatg gttgcattcc aggcactggt tgagttgacc gaacgcgcat ggaaaccacg
     6301 cggcatgcgg taataatcaa agaactcttt caataccgga ttagaattcg ctggcaaagt
     6361 ttccggtaaa atacgatcgg aaacggtgac gttgcccttc tcatcaacgt aaatatcatg
     6421 cccgcctggc gcgaaagcgc cattttcagc atccttccag cgctgcatgt tcaaatactg
     6481 caatacggca cgacgatcgg cagtggtata gcggtctttg ccatcgccca cgccatgccc
     6541 cattgcacgg ctcatgtcat acatcacgct gctcgccacc gccttgacac gtgtgtccat
     6601 cgcggcgtcg tttaaggcca tgccacccca tccgcagatg ccgagcaacc cgatacggtt
     6661 gcgatccacc tctttttgca ggcctaagaa atcaaccgcc gcgctgaaat cctcggtgtt
     6721 gatatccgga gatgcgacgt ttcgcgggta gccaccgctt tcacccgtgt atgaagggtc
     6781 aaaagccaga gtgacaaacc cccgttcagc cagcgtctgc gcatacaggc cgctggattg
     6841 ctctttcacc gccccgaagg ggccactaac agcaattgct gccagcttgc gatcgccacg
     6901 atccttcggt atatagagat ccccgactaa ggtgatcccg taccggttcg gaaaggtcac
     6961 cttacggtga tcaactttcg tgctctcagc aaaagtttta tcccatttct cgacctgcga
     7021 aacgggcgcg ttcggattgg ttgtatcagc gtaactcata tttgtgactc cacttaatga
     7081 tacgcataac agcatggtgg gtatcgcggc tgtcaatttt tgggtgaaag cagtcatcaa
     7141 aatccccctc ataaaatatg ccagatactg gtttcatacg gataacactg ctgtgctttg
     7201 ttgcaacgcg cacattatag ttagcattaa tagtgctaat tagagggtaa aaaatgctaa
     7261 gattaataac atattgttct gaattgtgat gcagcagggg aaggatgatg gcgaaacggg
     7321 aaaactataa cgatctctat ctgtttatgc aggtcgtgcg agaagggagt tttaccgccg
     7381 ccgcgcagag gcttggcctg gcgcaatcag gaattagccg tgcagtgcgc gagctggaag
     7441 agcgactggg cgtgcagctt ctggtacgca ccacgcgcaa gttatcgctc acccatgccg
     7501 gcgagttact ctatcgaaac gtcgaatctg gatttgacgc cttagatatg gggcttgcca
     7561 cactggccca ttatcgccat acaccatcgg gtacggtccg aattaatgcg agccagcacg
     7621 ccattgataa agtgctgctg ccgaagctgg cggtgtttaa acaacgatac ccggatattc
     7681 ggctggagct catcagcgaa agccggtttg tcgatattat tactgagcgc ttcgatgccg
     7741 gtatacgtct gggctcggaa gtggatagcg gtatggttgc ggtgcgcatt tcgcccgata
     7801 tggagatggc tgttgtcggc acgcccgggc actttcagcg ctacggattt ccacaaaccc
     7861 ccaccgattt agtaggtcat ccctgcatcg cttaccagtt cggcgacggc agtctgtatg
     7921 cctgggaact caatcaggac ggtaaaaaga tcacccatca gcctcaggga caatgggcct
     7981 ttgctgacag ttatatggaa gccaaagccg caaggctagg tctggggctg gcttatgttc
     8041 cggaggaatt agtgtctgat gatatagaac agggggcgct aatcagagtt ttgcaatgct
     8101 acagccaacg cctggagggt tcatatatct attatccgca ccgcaatgtg tcacccgccc
     8161 tacgagcggt tatcgataca ttgagaattt aaggggtgaa gatgagatgg ttcgcaggct
     8221 taggtaatag ttggaaaaca atgtccgctc ctggcacaga gcggactgtc agattagatt
     8281 cagacctgta ctcattagct gatacaaatc agccacatat acctgcattc caaataattt
     8341 caggcagata ttatctccgt gaatcccccc gccatctttc cggtgtgcgg ggttttgtct
     8401 tttttcaccg ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg
     8461 ctctcctccc tgccttactc actttcccgt cacatcctgg aacatctgcg caaactcacc
     8521 agtcacgaac ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt
     8581 aatgcgctgt ttcaggggga ggtcacaccg gtcagtgatg ttcacgccgg cacccgggaa
     8641 gtgcagcgct tccgtctgag tggccacggt cacagcatga ttatcactga cctgcccggg
     8701 gtgggcgaga gccgggacag ggatgcagag tatgaagccc tgtaccgtga cattctgcct
     8761 gaactggacc tggtactgtg gctgattaaa gccgatgacc gtgccctgtc tgtggatgag
     8821 tatttctggc gacacatcct gcagtgcgga catcagcagg tgctgtttgt ggtgacgcag
     8881 gccgataaaa cggagccctg ccatgaatgg gatatggccg gtattcagcc ttcccccgca
     8941 caggtacaga acattcgcga aaaaacggag gcggtattcc ggctgttccg gcccgtacat
     9001 ccggttgtgg ccgtatcggc ccgcaccggc tgggaactgg atacactggt cagtgcgctc
     9061 atgacagcac ttcccgacca tgccgccagc cccctgatga cccgcctgca ggacgagctg
     9121 cgcacggaat ctgtccgggg gcaggcccgt gaacagttta ccggtgcggt ggaccggata
     9181 tttgacacgg tggagagcgt ctgtgttgcc tctgttgccc gtacggttct gcgtgccgtc
     9241 cgtgacacgg tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacactc
     9301 tgccagggga aatgcctgat tacattgcgt taaccaactg aaaacatgac acaaatacct
     9361 caccccctcc tgtttctctt gcgtgaccgc cctgctatca atagaataaa acgatcgata
     9421 aaacaggtat cgatcgttac tatcgatcgt ttatagtggt taatggtctg cgaaaaggca
     9481 gctgaatctc ttcattatgc agaacggaat tgcacacaac ggactgatac ttctctggcg
     9541 gatgagagag gggaagcatt tatgtccggg aaaaaacact ttatctgacc atgctgtttt
     9601 gtacctgccg gtatccacat ttgtgggtac cggctttttt attcaccctc tgtaaggaaa
     9661 agctgatgaa acgacatctg aacaccagct acaggctggt atggaatcac attacgggca
     9721 ccctggtggt ggcctccgaa ctggcccgct cacggggaaa acgcgccggt gtggcggttg
     9781 ctctgtctct tgctgctgtc acgtcagtcc cggcactggc tgctgacacg gttgtacagg
     9841 cgggagaaac cgtgagcggc ggaacactgg aaaatcatga caaccagatt gtcttcggta
     9901 cggccaacgg aatgaccatc agtaccgggc tggagtatgg gccggataac gaggccaata
     9961 ccggcggaca atggatacaa aatggcggta tcgccaacaa cactactgtc accggtggtg
    10021 gtcttcagag agtgaatgcc ggaggaagcg tttcagacac ggttatcagt gccggaggcg
    10081 gacagagcct tcaggggcag gcagtgaaca ccactctgaa cggcggtgag cagtgggtac
    10141 atgaaggcgg gattgcaacg ggtaccgtca ttaatgagaa gggctggcag gccgtcaaat
    10201 ccggcgcagt ggcaaccgac acggttgtga ataccggcgc ggaaggggga ccggatgcgg
    10261 aaaatggtga taccgggcag accgtctacg gagatgccgt acgcaccacc atcaataaaa
    10321 atggtcgtca gattgtggct gctgaaggaa cggcaaatac cactgtggtt tatgccggcg
    10381 gcgaccagac tgtacatggt cacgcactgg ataccacgct gaatgggggg taccagtatg
    10441 tgcacaacgg cggtacggcg tctggcactg ttgttaacag tgacggctgg cagattatca
    10501 aggaaggtgg tctggcggat ttcaccaccg ttaaccagaa aggcaaactg caggtgaacg
    10561 ccggtggtac agccacgaat gtcaccctga cgcagggcgg cgcactggtc accagtacgg
    10621 cggcaaccgt caccggcagc aaccgtctgg gcaatttcac ggtggaaaac ggtaagactg
    10681 acggcgttgt tctggagtcc ggcggtcgcc tggatgtact ggagggccat tcagcgcaga
    10741 aaacacgggt ggatgacggc ggtaccctgg cagtgtctgc cggtggtaag gcaacaggtg
    10801 tcaccatgac atccggtggt gccctgatag cagacagtgg tgccactgtt gaggggacca
    10861 atgccagcgg taagttcagt attgatggta tctccggtca ggccagcggc ctgctgctgg
    10921 aaaatggcgg cagctttacg gttaatgccg ggggacaggc cggcaacacc actgtcggac
    10981 atcgtggaac actgacgctg gctgccgggg gaagtctgag tggcagaaca cagctcagta
    11041 aaggcgccag tatggtactg aatggtgatg tggtcagtac cggcgatatt gttaacgcag
    11101 gagagattca ctttgataat cagacgacac aggatgccgt cctgagccgt gctgttgcaa
    11161 aaggcgactc cccggtaacg ttccataaac tgaccaccag taacctcacc ggtcagggtg
    11221 gcaccatcaa tatgcgtgtt cgccttgatg gcagtaatgc ctctgaccag ctggtgatta
    11281 atggtggtca ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagtaacc
    11341 tcggggtggc aacctccgga cagggtatcc gggttgtgga tgcacagaat ggtgccacca
    11401 cagaagaagg tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc
    11461 tgaaccgtga cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag
    11521 tccccctgta tgcctccatg ctgacacagg caatggacta tgaccggatt ctggctggct
    11581 cccgcagcca tcagaccggt gtaaacggtg aaaataacag cgtccgtctc agcattcagg
    11641 gcggtcatct cgggcacgat aacaacggcg gtattgcccg tggggccacg ccggaaagca
    11701 gcggcagcta tggcttcgtc cgtctggagg gtgacctgct cagaacagag gttgccggta
    11761 tgtctctgac gacaggggtg tatggtgctg caggccattc ttccgttgat gttaaggatg
    11821 atgacggcta ccgcgccggc acggtccggg atgatgccgg cagtctgggc ggatacctga
    11881 atctggtaca cacatcctcc ggcctgtggg ctgacattgt ggcacaggga acccgccaca
    11941 gcatgaaagc gtcatcggac aataacgact tccgcgcccg gggctggggc tggctgggct
    12001 cactggaaac cggtctgccc ttcagtatca ctgacaacct gatgctggag ccacaactgc
    12061 agtacacctg gcagggactc tccctggatg acggccagga taacgccggt tatgtgaagt
    12121 tcgggcatgg cagtgcacaa catgtgcgtg ccggtttccg tctgggcagc cacaacgata
    12181 tgacctttgg cgaaggcacc tcatcccgtg acaccctgcg cgacagtgcc aaacacagcg
    12241 tgagtgaact gccggtgaac tggtgggtac agccttctgt tatccgcacc ttcagctccc
    12301 ggggagacat gagcatgggt acagccgcag ccggcagtaa catgacgttc tcaccgtccc
    12361 agaatggcac gtcactggac ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca
    12421 ccctgggcgt tcaggccggt tatgcccaca gtgtcagcgg cagcagcgct gaaggttata
    12481 acggtcaggc cacactgaac gtgactttct gataatcagg tcactcccct tcaggccccg
    12541 gtcatcatta ccggggcctt ttacttacag ggagcaacag atatgccggg ttgtacatca
    12601 cgtttattgc ccgaaggccc tttctcacgc aaccaggcac ttgccgtcac aacggcttac
    12661 cttaatgtgc ttattgaaga tgaccaggga acacatttcc ggttggttat tcgcaatgct
    12721 gaagggcagc tacgctggcg gtgctggaat tttgaacctg atgccggaaa acagctaaat
    12781 ccgtatctcg ccagtgaggg aattctcagg caataaacgt cattcacttg atccatcagg
    12841 ccgcgtcttc tccagaagac gcggcttttt catttatacc gctaactcat tcataaggag
    12901 caaatcatgc gattagcaag tcgttttggt catgtaaacc agatgctgcc gggttacttc
    12961 aggttcgtct gccagaacgg gtgcgtctgt ggccagtctc tgggggaagt gcgtgttcca
    13021 caccggggaa atgtagtgga gaaagttatc gaaggggctt acgaggtggt gggcgtgttt
    13081 gaccggatag aggagaagcg tgatgccatg cagtcgctgg tcctgccgcc accggcacgc
    13141 caggcgctgg cacaggcggc cctgacttac cgttatggtg acgaacatca gcccgtcacc
    13201 accgctgaca ttcttacgcc acgacgcagg gaggattacg gtaaggacct gtggagcgca
    13261 tatcagacca tccaggagaa tatgctgaaa ggcggaattt ccggccgcag tgccagagga
    13321 aaacgtatcc atacccgggc cattcacagc atcgacaccg atattaagct caatcgcgca
    13381 ttatgggtga tggcggaaac actgctggag agcatgcgct gatgcctttt ccctgaaagc
    13441 gcagtcctga ctcacgtctg tccctgcctc cggacattct tccattcatt tactttttat
    13501 aaggaataat ctcatgacaa ccgttacgca taattccacc acaccttctg tttccgtaac
    13561 tactgcatca gggaataacc cacctcagct ggttgctact ctcgtccctg atgaacagcg
    13621 catcagcttc tggccgcagc attttggcct cattccacag tgggtgaccc tggagccccg
    13681 tgtcttcggc tggatggacc gtctgtgcga aaactactgc ggtggtatct ggaatctgta
    13741 cacactgaat aacggagggg catttatggc acccgaaccg gatgacgatg atgacgaaac
    13801 atgggtactg ttcaatgtca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg
    13861 cattgctgcc tgtctgatga cgtacagtca tcatgcctgt cgtacggagt gttatgccat
    13921 gacggtccat tattaccggc tgcgggatta cgccctgcag catccggaat gcagcgccat
    13981 tatgcgcatc atcgactgaa tggagagaag cacaatgcaa caactttcct ttctgccagg
    14041 agagatgacg cccggcgagc gcagtctcat tctgcgggcc ctgaaaaccc tggaccgcca
    14101 tcttcatgaa cccggcgtgg ccttcacctc cacccgtgcg gcacgggaat ggctgattct
    14161 gaacatggcg ggactggagc gtgaagagtt ccgggtactg tatctgaata accagaatca
    14221 gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag tccatccccg
    14281 ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca
    14341 cccgtccggt gaagtcacac ccagcaaggc agaccgcctt atcacggaac gtctggtaca
    14401 ggcactgggc ctggtggata tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt
    14461 tttctccttt gccgaacatg gtctgcttta acccgtcaca accacatcac acctgttttc
    14521 acttttatct tctgtcttca gaggtatccc attatgaaaa ttatcacccg tggtgaagcc
    14581 atgcgtattc accaacaaca tccgacatcc cgtctttttc cgttctgtac cggtaaatac
    14641 cgctggcacg gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg
    14701 ctggccgtgt ttgctgaacg ccgtaaggac agttttggcc cgtatgtccg gctgatgagt
    14761 gtcaccctga actgatgttc cgtcacccgg gaaacgagta cgaccagatg actttagacg
    14821 ccgctgtgga tgaggccatt ctcatcaatg catacatctt caccgaaacc ggtcagcgcg
    14881 ccggctgacc tgtcatcgtt aaaccattct taaccacttt tcagagagga ttttatcgtg
    14941 tcagacacac tctccgggat aactcatccc gacgacaatc acgaccgccc ctggtggggg
    15001 cttccctgca cagtgaggcc ctgttttggc gcccgtctgg tgcaggaggg taaccgcctg
    15061 cattaccttg ccgaccgcgc cggtatcaga ggccgcttca gagacgcaga tgcataccat
    15121 ctggaccagg cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa
    15181 ctgaatcccc gacatcagca taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc
    15241 gacaccctcg gctcctgtgg ttacgtttat ctgactgttt atccgacacc ggcacccgca
    15301 accacctcat aaccccgaag ctctcacctt tagcactacc agttacagag aacttaagat
    15361 gaaaacatta cctgacacac acgtacggga ggcatcgcgt tgcccgtctc ccgtcaccat
    15421 ctggcagaca ctgctcgccc gactgctgga ccagcactac ggcctcacgc tgaatgacac
    15481 tccgttcgct gatgaacgtg tgattgagca gcatattgag gccggcattt cactgtgcga
    15541 tgcagtgaac tttcttgttg aaaaatacga actggtgcgt accgaccagc cggaattcag
    15601 tgcctgtacc cgttctcagt taataaacag catcgatatc ctccgggctc gcagggcgac
    15661 cggcctgatg acccgcgaca attacagaac ggtaaataac attaccctgg gtaagtatcc
    15721 ggaggcgaaa tgatgaaact ggccctgaca ccggaagccg acagcgttaa cgtacaggca
    15781 ctgaatatgg ggcgaattgt cgttgacatt gatggtgtta atctcgctga actgattaac
    15841 atggtctgcg ataacggcta ctcgcttcgt gttgttgatg aatctgaccg gacctcagca
    15901 gactgcacgc caccgtttgc tgcccttacc ggcatacgct gcagtaccgc gcatatcacg
    15961 gcaaaggaca acgcctggct gtactcgctg tcgcatcaga ccagtgacgt tggtgaatcg
    16021 gaatggattc atttcacagg tagcggatat ctgttacgta ccgatgcgtg gtcatacccg
    16081 gttctgcggc ttaagcgcct gggcctgtca aaaacgttcc gtcgtctggt tgtcacactt
    16141 acccgacgtt atggcgtcag tctcattcat ctggatgcca gcgctgaatg cctgccgggt
    16201 ttacccactt tcaactggta cccaggaaca acatgaaatc attaaccacg gaaacagcac
    16261 tggatattct gattgcgtgg ctgcaggaca atatcgactg cgaaccggga attatctttg
    16321 acaacgatga ggataaaacg gattcggcag cactgctgcc ctgcattgaa caggcgaggg
    16381 aggatatccg taccctgcgc catctgcagc ttcagcacca gaaccggtga agctcactca
    16441 ccagacttca ttcctctgac gccagcctga acagggctgg cattttcatt tatctgcaaa
    16501 aaggaatatc gattatgtca gatatcacaa tctcccgtcc ggaagtggtc aacgggcata
    16561 cggacgttat ctgctcaacc agcattcgcc acattctggc tgtacgaaag agtacactgt
    16621 tgcaaatcga cacacttatc cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg
    16681 gaggtaaaac tgccccggac tgggccatga aacaggattt tcgctgcggt tcctagctga
    16741 tggagacacc agaagcagca atgaaagcca ttacccggaa tcttgatcgc gaaatctggc
    16801 gtgacctgat gcaacattcc gggatgctgt cattaatgga tacgcaggcc cgtgatacat
    16861 ggtaccggtc actggagtac gataattttc cggaaatcag cgaagcgaac attctgagca
    16921 catttgaaca actgcaccag aacaaggatg aggtgtttaa gcggggagtg attaacgtct
    16981 tcagggaact gagctggaat tataaaacca actctccctg taaatttgga agtaaaatca
    17041 ttatcaacaa tctggtgaga tgggaccggt ggggatgtca tcttatcacc ggacaacaga
    17101 ctgaccgact cgccgacatg gaaagaatgc tacatctgtt cagcggcaaa ccaatccccg
    17161 acaaccggga aaacatcact attcgtctgg atggtcacat ccagtccgtt cagggtaaag
    17221 agcgctatga aaacgagatg ttcatcatta aatattttaa gaagggatct gcacacatca
    17281 ctttcaaaag gctggaactg attgacagaa ttaacgatat aatagccagg caatttcttt
    17341 ctgtgctctc agcctgatcc cgagtgtgat tccctttcaa tatcaaaagg gactgcgggt
    17401 acaaatgagg gtacattttt aatcaaacca aacaaatcag atttatatca atatgataga
    17461 ggcaaccttc gattccgagt ccggcaccac tattctaaag aacccgccca caaggcgggt
    17521 ttttgctttt ggatctttat ataaaataaa cttgggagca taacgccatc aggtcttaaa
    17581 cactaccgaa tcatcaataa acatcccccg ttaacaaaac acagttcagt tcacataaca
    17641 tccctgttat cccacacata tctgctggcc gaacgacgcc agtgcgcgta cggaggcaag
    17701 agcagccctg cacatggcag cgcttgtggt aacctggttt aatccggtga taaaggcgtt
    17761 ttacatgcat ctgtcagcag ccggaaaaac caaaaaagtg gcgctggttg actgtatgcg
    17821 taaattgctg accatcctga acgcaatgct cagaaagaac gaagagtgaa atgaatcgta
    17881 gcatcatgtt gctccataat tgttacgttc aagacagttg attatcggcg catatgactc
    17941 aaaaagagcc tgatgattgc ctcttttcca taggctcttt ctctgaagtg aaggaaggcc
    18001 cggaagaaag cactcgctgg catacgtgac tccagctcaa ccagagataa tcaccattgt
    18061 tatttttcta tttaataata atctcctgaa aaatagatat agcaaaaata caatccggcc
    18121 agatttaatt ccgcattcat taccagaaat aatcaacacc acaactatca tatcattcaa
    18181 tagtcaccgc taaaatttta ttattttcct cttctttaca tagaaattgt caacctgttt
    18241 attatgccgc acttaaatag ttaatcacaa aacaacatca ccattatttg cgtgttttat
    18301 ttattaataa catagtcata cgttcttttt ttacaaggaa ataatcaatt gataaattgt
    18361 ttttggtctt aatagtcaag caggaaacat tttaataaat gataaaaatc ctaaactcct
    18421 tgttcataat gtaggggtgt gttaaatagg taggcaaaat ttggttcccc ttctcgcctg
    18481 taatattgca gcatcttcgc acctccatga gacattgcga cttaataaga aggtgataag
    18541 tcctgcatat aagcatggac tgaccatggt tttatattca ttaaaaattt tgttacaacc
    18601 cattgattta gcgtaaataa attatagtgg gttcgggttt gttgtgactg tggcattatt
    18661 tccgtgcaaa ggagctgata tgtctgaaag acatttacct gatgaccaga gcagtactat
    18721 cgatccatat ctaattacct ctgttcgcca gactctggca gaacaaggcg cagcattaca
    18781 aaacttgtct aaacaactgg attccgggca gtaccagcgt gtccttaatt tgataatgaa
    18841 ctgtaaaggt cacgttattc tttcgggaat gggtaaatca gggcatgtcg gtcgtaaaat
    18901 gtcagcgacg ctggcctcta cgggtacgcc tagtttcttt attcatcctg cagaagcttt
    18961 ccatggcgat ctgggcatga ttacgcctta cgatcttctg atccttattt ctgccagcgg
    19021 tgaaacggat gaaatcctca agctagttcc ttcactgaaa aatttcggca accgaattat
    19081 cgctattacc aataatggaa attccacgct ggcgaaaaat gctgatgccg tgctggaact
    19141 ctacatggcg aatgaaacct gcccgaataa tcttgcacca acaacgtcta ccacgctgac
    19201 gatggcgatc ggcgatgcgc tggcgattgc catgatccac caacgcaaat ttatgccgaa
    19261 tgattttgcg cgctatcacc cgggcggttc attaggacgt cgcctgctga cccgcgttgc
    19321 agatgtcatg cagcatgatg tcccttcggt tcacctggat gcgccattta aaacggtaat
    19381 tcaacgtatc accagcggtt gccagggaat ggtgatggta gaagacgcag aaggcgagtt
    19441 ggcgggaatt atcaccgacg gtgacctgcg tcgctttatg gaaaaagagg attctctgac
    19501 atccgccacg gcggcgcaga tgatgacgcg tgaaccgctg acgttgccag aagaaaccat
    19561 gatcatcgaa gcggaagaaa agatgcaaaa acaccgcgtc tcgacgttat tggtgaccaa
    19621 caaggcgaac aaagtcactg gtcttgtgcg cattttcgac taattaagca acagggcgcg
    19681 agagtttagt ctctcattga atatgcgccc cactgaaact ctgataatcg ttacatgttg
    19741 ataaaagtga agtctgccgt atcctggatg cgtgctcgtt tgtctgccat ctcactggct
    19801 gatatccaaa aacacctggc gaaaatcatc attctggcac cgatggcagt gctgctgatc
    19861 tatctggcta tcttcagcca gcctcgctat atgagcgagt cgaaagttgc cattaaacgc
    19921 tcagatgatt taaacagcgg cagcttaaat tttggtctgc tattgggtgc ctctaacccg
    19981 agttccgcag aggatgcgtt atatctgaaa gagtacatca actctccaga tatgctggca
    20041 gcgttagata agcaactgaa ttttcgcgaa gcgtttagcc acagcgggct cgattttctt
    20101 aatcatctta gcaaggatga aaccgcagaa ggcttcctga agtactacaa ggaccgtatc
    20161 aacgtctcgt atgacgataa aaccggattg ctcaatattc agacgcaggg ctttagcccg
    20221 gagtttgcgc ttaagtttaa ccagaccgtg ctgaaagagt cagagcgctt tatcaatgag
    20281 atgtcacatc gcatcgcgcg tgaccagctt gcctttgcag aaacggagat ggaaaaggca
    20341 cgccagcgtc tggacgccag caaagcggaa ttgctctctt atcaggacaa caacaacgtt
    20401 ctggatccac aggcacaggc acaggcggcg agcacgttag tgaatacgct gatgggccag
    20461 aagatccaga tggaagcgga tctgcgtaac ttgctgacgt atctgcgtga ggacgccccg
    20521 caagttgtga gtgcgcgtaa tgcgattcag tcattgcagg cacaaattga cgaagaaaaa
    20581 agcaaaatca cagcgccgca gggtgacaag ctaaaccgta tggcggtgga ttttgaagaa
    20641 atcaaatcaa aagtagagtt caacaccgag ctgtacaaac tgaccctgac ctccattgaa
    20701 aagacccgtg tagaagcggc tcgtaagctc aaggtgctgt cagtgatcag ttcgccacag
    20761 ttgccgcagg aatcgtcttt tccaaatatc ccttatttga tcgcctgctg gttactggtg
    20821 tgctgcctgc tgttcggcac cctgaaactg ttgctggctg ttattgaaga tcaccgagac
    20881 taacgctgtc gctgaatgag tttgtgatga aattatttaa atcaatttta ctgattgccg
    20941 cctgtcacgc ggcgcaggcc agcgcggcca ttgatattaa cgctgaccca aaccttacag
    21001 gagccgcgcc gcttaccggt attctgaacg ggcaacagtc ggatacgcaa aacatgagcg
    21061 gcttcgacaa taccccgccg cccgcaccgc cggtggtaat gagccgtatg tttggtgctc
    21121 aacttttcaa cggcaccagc gcggatagcg gtgcgacggt aggattcaac cctgactata
    21181 ttctgaatcc gggtgatagc attcaggttc gcttgtgggg tgcgttcacc tttgatggtg
    21241 cattacagat tgatcccaaa ggtaatattt ttctgccaaa cgttggtccg gtgaaagttg
    21301 ccggggttag caatagtcag ttgaacactc tggttacatc aaaagtgaag gaagtgtacc
    21361 agtccaacgt caacgtctac gcctccttat tacaggcgca gccagtaaaa gtgtacgtga
    21421 ccggatttgt gcgtaatcct ggtctgtatg gcggtgtgac gtctgattcg ttactcaatt
    21481 atctgatcaa ggctggcggc gttgatccag agcgcggaag ttacgttgat attgtggtca
    21541 agcgcggtaa ccgcgtgcgc tccaacgtca acctgtacga cttcctgctg aacggtaaac
    21601 tggggctttc gcagttcgcc gatggtgaca ccatcatcgt cgggccgcgt cagcatactt
    21661 tcagcgttca gggcgatgtc tttaacagct acgactttga gttccgcgaa agcagcattc
    21721 ccgtaacgga agcgttgagc tgggcgcgcc ctaagcctgg cgcgactcac attacgatta
    21781 tgcgtaaaca ggggctgcaa aaacgcagcg aatactatcc gatcagttct gcgccaggcc
    21841 gtatgttgca aaatggcgat accttaatcg tgagcactga ccgctatgcc ggcaccattc
    21901 aggtgcgggt tgaaggcgca cactccggtg aacatgccat ggtactgcct tatggttcca
    21961 ctatgcgtgc ggttctggaa aaagtccgcc cgaacagcat gtcgcagatg aacgcagttc
    22021 agctttatcg cccatcagta gctcagcgtc agaaagagat gctgaatctc tcgctgcaaa
    22081 aactggagga ggcctcactt tctgcccagt catccaccaa agaagaagcc agcctgcgaa
    22141 tgcaggaagc gcaactgatc agccgctttg tggcgaaagc gcgcaccgtg gttccgaaag
    22201 gtgaagtgat cctcaacgaa tccaatattg attctgttct gcttgaagat ggcgacgtca
    22261 tcaatattcc ggagaaaaca tcgctggtta tggttcatgg cgaggtgctg ttcccgaacg
    22321 cggtgagctg gcagaaaggg atgaccaccg aggattacat cgagaaatgc ggtggcctga
    22381 cgcaaaaatc gggtaacgcc agaattatcg tcattcgtca gaacggtgcg gcagtcaacg
    22441 ctgaagatgt agattcactc aagcctggcg atgagattat ggttctgccg aaatatgaat
    22501 cgaaaaacat tgaagttacc cgtggtattt ccaccatcct ctatcagctg gcggtgggtg
    22561 caaaagtgat tctgtctttg taaggggttg aaatgagcaa agcagttatt gtcattccgg
    22621 ctcgctatgg ctcctcgcgc ctgccgggta agccactgct cgatattgtt ggtaaaccga
    22681 tgatccagca tgtttacgag cgtgcgttac aggtggcggg cgttgcggaa gtttgggtgg
    22741 caacagacga tccgcgtgtt gaacaggccg tacaggcgtt tggcgggaaa gccatcatga
    22801 cgcgcaacga tcatgaatcc ggcaccgatc ggctggtcga ggtgatgcat aaagtcgagg
    22861 cagatatcta cattaacctg cagggcgacg aaccaatgat tcgcccgcgg gatgtagaaa
    22921 cgctgttaca aggaatgcgt gatgaccccg cgttgccagt ggcaacgcta tgccacgcga
    22981 tttctgccga agaagcggct gagccaagca cggtaaaagt ggtggtaaat acccgccagg
    23041 atgcgcttta tttcagccgt tcgcctattc tgtatccgcg taatgctgaa aaagcgcgct
    23101 acctgaaaca cgttggtatt tacgcttatc gtcgcgatgt gctgcaaaac tacagccagt
    23161 taccggagtc catgcaggag caggcagaat cactggagca gctgcggttg atgagtgcgg
    23221 ggatcaacat ccgcacattt gaggttgccg caaccggtcc gggcgtcgac accccagcat
    23281 gcctggaaaa agtgcgcgcc ctgatggcac aggaactggc tgaaaacgca tgattggcat
    23341 tttctcgtcc ggtatctggc gtattccgca tctggagaaa tttctggcgc aaccgtgcca
    23401 gaaactttct ctgctgcgcc ctgttccgca agaagttgat gctatcgccg tgtggggaca
    23461 tcgtcccagc gcggcgaaac cagtcgccat cgccaaagca gcgggaaaac ccgtcattcg
    23521 tctggaagat ggatttgtac gttcgctgga tcttggcgtc aatggcgagc cgccgctttc
    23581 tctggtggtg gatgattgtg gcatttacta cgatgccagc aagccttcag cactggagaa
    23641 actggtacag gataaagccg gaaatacagc tctgataagc caggccagag aagcgatgca
    23701 caccatcgtg accggggatt tgtcgaaata taacctggca cctgcgtttg tggcggatga
    23761 atcagaacgt tcagacatcg ttctggttgt cgatcagaca tttaatgata tgtcagtgac
    23821 gtatggcaat gctggcccgc atgagtttgc tgccatgctg gaagccgcga tggcggaaaa
    23881 tcctcaaacc gaaatttggg tgaaggtgca cccagatgta ctggaaggaa agaaaacagg
    23941 ttatttcgct gatctgcgcg ccacgcaacg agtacgtttg attgctgaga atgtcagccc
    24001 gcagtcgctg ttgcgacacg tttcccgggt ttacgtcgtg acctcccagt atggctttga
    24061 agccttgctg gcaggaaaac cagtaacatg tttcggccag ccctggtatg caggttgggg
    24121 cttaaccgac gatcgtcatc cacagtccgc tttgttatct gcccgacgcg gttctgccac
    24181 gctggaggaa ctttttgccg ctgcatacct gcgttactgt cgctatatcg atccgcaaac
    24241 gggagaagta agcgatatat ttaccgtact gcaatggctg caattacagc gcaaccatca
    24301 gcaacagcgt aatggctatt tatgggcgcc aggcttaacg ctgtggaagt cagcgatcct
    24361 gaaacctttc ttgcgaacag cgacaaaccg gctgagtttt tcacgtcgtt gtactgcggc
    24421 gagcgcctgc gtggtatggg gtgtaaaggg agaacagcaa tggcgagccg aagcgcaacg
    24481 aaaatcactg ccgttatggc gaatggaaga tggttttctg cgttcatccg gacttggctc
    24541 tgaccttttg ccgccgctat cgttggtgct ggataaacgc gggatctact atgacgccac
    24601 gcgccccagc gacctggaag tgctgattaa tcacagccag ttaacactgg cgcagcagat
    24661 gcgagctgaa aaattacgcc agcgactggt tgaaagtaaa ctgagcaagt acaacctggg
    24721 ggccgatttc tctctaccag ccgaagccaa agataaaaaa atcatcctgg tgccgggtca
    24781 ggtagaagac gatgcctcaa ttaaaaccgg caccgtttct attaaaagta accttgagtt
    24841 attgcgtacc gtacgtgaac gtaacccgca cgcctacatt atttataaac cgcacccgga
    24901 tgtattagtg ggaaatcgca agggcgatat tccggcagaa ctgattgctg aacttgctga
    24961 ttatcaggca ctggacgcag atattattca atgcatccag cgcgcaaatg aagtgcatac
    25021 catgacatca ttgtccgggt ttgaagcgtt attacatggc aagcaagttc attgttacgg
    25081 cctgcccttc tatgccggtt ggggtttaac cgttgatgaa catcactgcc cgcgccgcga
    25141 gcaaaaatta acgttagcag atttgatcta tcaggcgttg attgtttatc caacctatat
    25201 ccacccaaca cggctacaac ctattacggt tgaagaagct gcggaatatt taatcaagac
    25261 gccgcgcaag ccgatagtaa ttagaaaaaa taatattgac agagttatta atatttaccg
    25321 taagttaatc atgttctgca gagtcagact gggttaacca atttcattat tgactattgc
    25381 acagattatt tatgcaaggt aatgcactaa ccattttatt atcgggtaaa aaatatctgc
    25441 tattgcaggg accgatggga ccttttttca atgacgttgc cgaatggtta gagtcattag
    25501 gccgtaacgc tgtgaatgtt gtgttcaacg gcggtgatcg tttttactgc cgcaatcgac
    25561 aataccaggc ttacaaccaa acgccgaaag aatttcccgg ttggttgcga gatctccacc
    25621 ggcaatatga ctttgatacc atcctctgct ttggtgactg ccgcccatta cacaaagaag
    25681 caaaacggtg ggctaagtcg aaagggatcc gctttctggc atttgaggaa ggatatttac
    25741 gcccgcaatt tataaccgtt gaagaaggcg gagtgaacgc atattcatca ctaccgcgcg
    25801 atcctgattt ttatcgtaag ttgccagata tgcctgcgcc gcacgttgag aacttaaaac
    25861 cttcaacgat gaaacgcatt ggccatgcga tgtggtatta cctgatgggc tggcattacc
    25921 gtcatgagtt ccctcgctac cgccaccaca aatcgttttc cccctggaat gaggcacgtt
    25981 gctgggttcg tgcatactgg cgcaagcaac tttacagggt aacacagcgt aatgtattgc
    26041 cgaggttaat gaatgagctg gaccagcgtt attatcttgc cgttttgcag gtgtataacg
    26101 atagccagat tcgtaaccac agcaattata acgatgtgcg agactatatt aatgaagtca
    26161 tgtactcatt ttcgcgtaaa gcgccgaaag agagttattt agtgatcaaa catcatccga
    26221 tggatcgtgg tcacagactc tatcgaccat taattaagcg gttaagtaag aaatatggct
    26281 taggtgagcg cgtcatttat gtgcacgatc tcccgatgcc ggaattatta cgccatgcaa
    26341 aagcggtggt gacaattaac agtacggcgg ggatctctgc gctgattcat aacaagccac
    26401 tgaaagttat gggaaattct ctttatgata tcgttgactt aa
//
LOCUS       DARNVM010000057        26428 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.057, whole genome shotgun sequence.
ACCESSION   DARNVM010000057 DARNVM010000000
VERSION     DARNVM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.057"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="SI621_002116"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="SI621_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEI4064836.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="SI621_002117"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="SI621_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEI4064837.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="SI621_002118"
     CDS             complement(941..1408)
                     /locus_tag="SI621_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEI4064838.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="SI621_002119"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="SI621_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEI4064839.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="SI621_002120"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="SI621_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEI4064840.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="SI621_002121"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="SI621_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEI4064841.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="SI621_002122"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="SI621_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEI4064842.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="SI621_002123"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="SI621_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEI4064843.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="SI621_002124"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="SI621_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEI4064844.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="SI621_002125"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="SI621_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEI4064845.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="SI621_002126"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="SI621_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEI4064846.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="SI621_002127"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="SI621_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEI4064847.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="SI621_002128"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="SI621_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEI4064848.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="SI621_002129"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="SI621_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEI4064849.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="SI621_002130"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="SI621_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEI4064850.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="SI621_002131"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="SI621_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEI4064851.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="SI621_002132"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="SI621_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEI4064852.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="SI621_002133"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="SI621_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEI4064853.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="SI621_002134"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="SI621_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEI4064854.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="SI621_002135"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="SI621_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEI4064855.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="SI621_002136"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="SI621_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEI4064856.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="SI621_002137"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="SI621_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEI4064857.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="SI621_002138"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="SI621_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEI4064858.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="SI621_002139"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="SI621_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEI4064859.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="SI621_002140"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="SI621_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEI4064860.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="SI621_002141"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="SI621_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEI4064861.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="SI621_002142"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="SI621_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEI4064862.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="SI621_002143"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="SI621_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEI4064863.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="SI621_002144"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="SI621_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEI4064864.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="SI621_002145"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="SI621_002145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEI4064865.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="SI621_002146"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="SI621_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEI4064866.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="SI621_002147"
     CDS             15548..15916
                     /locus_tag="SI621_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEI4064867.1"
                     /translation="MWLLDQWAERHITEAQSKGEFDNLPGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYKEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="SI621_002148"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="SI621_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HEI4064868.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESA
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="SI621_002149"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="SI621_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HEI4064869.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="SI621_002150"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="SI621_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEI4064870.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="SI621_002151"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="SI621_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEI4064871.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="SI621_002152"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="SI621_002152"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEI4064872.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     VLPGFEEGWVTVQDASAQGCMAWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="SI621_002153"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="SI621_002153"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEI4064873.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKALPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="SI621_002154"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="SI621_002154"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEI4064874.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="SI621_002155"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="SI621_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEI4064875.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVAGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="SI621_002156"
     CDS             22593..23066
                     /locus_tag="SI621_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEI4064876.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="SI621_002157"
     CDS             23095..23637
                     /locus_tag="SI621_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEI4064877.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="SI621_002158"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="SI621_002158"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HEI4064878.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGATGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="SI621_002159"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="SI621_002159"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEI4064879.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGSKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25034..25291
                     /locus_tag="SI621_002160"
     CDS             25034..25291
                     /locus_tag="SI621_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HEI4064880.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="SI621_002161"
     CDS             complement(25267..25821)
                     /locus_tag="SI621_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEI4064881.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25850..26086
                     /locus_tag="SI621_002162"
     CDS             25850..26086
                     /locus_tag="SI621_002162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064882.1"
                     /translation="MTLGALLALCKSFSMQKSTVLCANAAKRVKKQQTEKKDQKSTCA
                     KNWDPYNAPPLRRQRETLHKMVVTTKRKNPEIQG"
     gene            26295..>26428
                     /locus_tag="SI621_002163"
     rRNA            26295..>26428
                     /locus_tag="SI621_002163"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6796 a         6257 c         6831 g         6544 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagtttcgaa ttaaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaatttca ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc tatcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc taaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtctacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccagcc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccatgtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaact ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggta
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctattgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa accaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgtt gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat tccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct ttctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcatacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgtactgg
    10201 ctaaagcgta tggttccacc aacccgatta acgtggttcg tgcaactatc gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag gggggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaactac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aatcaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 accatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtaca ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgatttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat attactgagg cacaatccaa
    15601 aggtgagttt gataatcttc cgggaagtgg cgagccattg attctggatg atgattcaca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcggaggg aagcaataca gcttctggat atcctcaaag gtatccgtca
    15781 tgatgatccg caatataaag aggttagtcg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcag
    15961 aagtaacacc cgacacgatt cgttattacg aaaagcagca gatgatggag catgaagtgc
    16021 gcaccgaagg tgggtttcgc ctgtataccg aaagcgctct ccagcgattg aaatttatcc
    16081 gccatgccag acaacttggt tttagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatact tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaga
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgcaggcgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggct acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt tgaaatcgat cccgccaatt aataagccca gaggtggcat aatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaatc ataagtctaa caaatgttaa gctattttcc tgccaggaat
    17101 ggatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaaggact tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttca
    17341 tcaatgactc tgccgacaac gcgtgaggta ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cggaaatcgt tgcttgttgc ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcga gcatggctga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgttgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc aacaatggtc
    17941 gaaccttgcg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcattaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatacgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact agttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag taatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgcttaccc ggttgttctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatctggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgagaa gggtaacggc catcaccttg tttcacggtc
    18961 gccttcatac caaggcgttt taaattgtcg taaacccgag agaggcgctg ttcgtcgata
    19021 tcaaccgcaa caacctgcgc ttcgggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcagcgc aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggccatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccttctt caaaacctgg tagcacatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccagcaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttctacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgttt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgttggaact ggcgtaatac gccgttaatc
    19501 aaccctttaa gttgcggacg cttaattgcg acagcacctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acagtgcgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgtgccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaatgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agagtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acctgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acaaccataa catcagcctg tagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaaccggtt gaaaaacggg cagagctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcatcc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat cgacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa ctgggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttc ttggttggag caacccaacc atcatttaat
    21721 tcctgcggac agcgaatttt atcctcctca acttcttgcg acgacagatt accccggcgc
    21781 actgtttgtt gcaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattaggg aatggactta ataccattca
    22021 tccgcgtcgc catgcccgac tggctaccag tttgcttgaa catggtgggg ctcttgtctc
    22081 ggaatttccc ctcgatgttc ctccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtagt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccgg gtccattagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcaggaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gtaactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgaa gagtgtgaaa gactggatgc cagctgtcgt ggttttttgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatatcg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacgtttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgattct
    23821 aatcgccgca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgacgc
    23881 gcagcgtgaa actatatttt ctcgctggcc aggtcctgtc acctttgttt ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgcaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt agtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc attggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatctcgc gcagaagagt tgactaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 ccttccttct ttggcatggc gttctgcctg atgtagaacc ggtcatcaaa ctattgcaac
    25021 aggagttgtc agcgtgaatc aggccatcca gtttccggat agggaagagt gggacgagaa
    25081 taaaagatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggttgggtct ggttaccttg atccagatat tcgtccttcc atttcacgta
    25321 gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccaggct
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacgctg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tactcacctc aacaataaat gatttcctaa tgactttggg
    25861 tgcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaaa gatcaaaaaa gtacttgtgc
    25981 aaaaaattgg gatccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacaa
    26041 gatggtcgta acaacaaaga gaaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    26101 agcgtaatat acgccacctc gcgacagtga gctgaaagct gcgtcgcaac tgctctttaa
    26161 caatttatca gacaatctgt gtgggcactc gaagatacgg attcttaacg tcgcaagacg
    26221 aaaaatgaat accaagtctc aagagtgaac acgtaattca ttacgaagtt taattctttg
    26281 agcatcaaac ttttaaattg aagagtttga tcatggctca gattgaacgc tggcggcagg
    26341 cctaacacat gcaagtcgaa cggtaacagg aagcagcttg ctgctttgct gacgagtggc
    26401 ggacgggtga gtaatgtctg ggaaactg
//
LOCUS       DARNVM010000058        26135 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.058, whole genome shotgun sequence.
ACCESSION   DARNVM010000058 DARNVM010000000
VERSION     DARNVM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.058"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(254..334)
                     /gene="shoB"
                     /locus_tag="SI621_002164"
     CDS             complement(254..334)
                     /gene="shoB"
                     /locus_tag="SI621_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HEI4064883.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(754..1134)
                     /locus_tag="SI621_002165"
     CDS             complement(754..1134)
                     /locus_tag="SI621_002165"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEI4064884.1"
                     /translation="MAILGLGTDIVEIARIEAVITRSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(1134..1865)
                     /gene="pdxJ"
                     /locus_tag="SI621_002166"
     CDS             complement(1134..1865)
                     /gene="pdxJ"
                     /locus_tag="SI621_002166"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEI4064885.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEGQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1877..2605)
                     /gene="recO"
                     /locus_tag="SI621_002167"
     CDS             complement(1877..2605)
                     /gene="recO"
                     /locus_tag="SI621_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEI4064886.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2617..3522)
                     /gene="era"
                     /locus_tag="SI621_002168"
     CDS             complement(2617..3522)
                     /gene="era"
                     /locus_tag="SI621_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEI4064887.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3519..4199)
                     /gene="rnc"
                     /locus_tag="SI621_002169"
     CDS             complement(3519..4199)
                     /gene="rnc"
                     /locus_tag="SI621_002169"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEI4064888.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4472..5446)
                     /gene="lepB"
                     /locus_tag="SI621_002170"
     CDS             complement(4472..5446)
                     /gene="lepB"
                     /locus_tag="SI621_002170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEI4064889.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5462..7261)
                     /gene="lepA"
                     /locus_tag="SI621_002171"
     CDS             complement(5462..7261)
                     /gene="lepA"
                     /locus_tag="SI621_002171"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEI4064890.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7459..7938)
                     /gene="rseC"
                     /locus_tag="SI621_002172"
     CDS             complement(7459..7938)
                     /gene="rseC"
                     /locus_tag="SI621_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HEI4064891.1"
                     /translation="MIKEWATVISWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLMGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7935..8891)
                     /gene="rseB"
                     /locus_tag="SI621_002173"
     CDS             complement(7935..8891)
                     /gene="rseB"
                     /locus_tag="SI621_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HEI4064892.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8891..9541)
                     /gene="rseA"
                     /locus_tag="SI621_002174"
     CDS             complement(8891..9541)
                     /gene="rseA"
                     /locus_tag="SI621_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HEI4064893.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9574..10149)
                     /gene="rpoE"
                     /locus_tag="SI621_002175"
     CDS             complement(9574..10149)
                     /gene="rpoE"
                     /locus_tag="SI621_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEI4064894.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10146..10301)
                     /gene="rseD"
                     /locus_tag="SI621_002176"
     CDS             complement(10146..10301)
                     /gene="rseD"
                     /locus_tag="SI621_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HEI4064895.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10557..12179
                     /gene="nadB"
                     /locus_tag="SI621_002177"
     CDS             10557..12179
                     /gene="nadB"
                     /locus_tag="SI621_002177"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEI4064896.1"
                     /translation="MNTLPDHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGGGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNICVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12164..12901)
                     /gene="trmN"
                     /locus_tag="SI621_002178"
     CDS             complement(12164..12901)
                     /gene="trmN"
                     /locus_tag="SI621_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HEI4064897.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAKRINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            13033..14367
                     /gene="srmB"
                     /locus_tag="SI621_002179"
     CDS             13033..14367
                     /gene="srmB"
                     /locus_tag="SI621_002179"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HEI4064898.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14400..15281)
                     /locus_tag="SI621_002180"
     CDS             complement(14400..15281)
                     /locus_tag="SI621_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4064899.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIKDVDFTEPGRHNACSFIINEPQCVFRQLFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            15384..15971
                     /gene="eamB"
                     /locus_tag="SI621_002181"
     CDS             15384..15971
                     /gene="eamB"
                     /locus_tag="SI621_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HEI4064900.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCVGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRPLNIVLALLLVYCAVRIFY"
     gene            complement(16033..16416)
                     /gene="grcA"
                     /locus_tag="SI621_002182"
     CDS             complement(16033..16416)
                     /gene="grcA"
                     /locus_tag="SI621_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HEI4064901.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16721..17410
                     /gene="ung"
                     /locus_tag="SI621_002183"
     CDS             16721..17410
                     /gene="ung"
                     /locus_tag="SI621_002183"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEI4064902.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(17458..18495)
                     /locus_tag="SI621_002184"
     CDS             complement(17458..18495)
                     /locus_tag="SI621_002184"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEI4064903.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSHPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDEALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18702..19121
                     /gene="trxC"
                     /locus_tag="SI621_002185"
     CDS             18702..19121
                     /gene="trxC"
                     /locus_tag="SI621_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEI4064904.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19190..19888
                     /gene="tapT"
                     /locus_tag="SI621_002186"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19190..19888
                     /gene="tapT"
                     /locus_tag="SI621_002186"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEI4064905.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19920..22580
                     /gene="pat"
                     /locus_tag="SI621_002187"
     CDS             19920..22580
                     /gene="pat"
                     /locus_tag="SI621_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HEI4064906.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22694..24049
                     /gene="pssA"
                     /locus_tag="SI621_002188"
     CDS             22694..24049
                     /gene="pssA"
                     /locus_tag="SI621_002188"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEI4064907.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24095..24418
                     /locus_tag="SI621_002189"
     CDS             24095..24418
                     /locus_tag="SI621_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HEI4064908.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(24415..25713)
                     /gene="kgtP"
                     /locus_tag="SI621_002190"
     CDS             complement(24415..25713)
                     /gene="kgtP"
                     /locus_tag="SI621_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HEI4064909.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(26022..>26135)
                     /locus_tag="SI621_002191"
     CDS             complement(26022..>26135)
                     /locus_tag="SI621_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEI4064910.1"
                     /translation="LENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         6345 a         6861 c         6487 g         6442 t
ORIGIN      
        1 agacggaaga acagttgtgg gataaatttg tgctgatgca tcacgcggat aaaatttaat
       61 attctgctcc tggagtagac tattgttgac tttatagtgt gagccttcag gttcttatat
      121 ttattttcgt gatgtgatca taaatatcaa ttatttcatg aatgataaca ctatctgtat
      181 aagcacttat ttataattat taaaggcgcg cctggctggt taacctgacg cgccttaagt
      241 acgtttcctt atgttataag aacagaagga tcagctgtaa aacagcaatg atgattttga
      301 tgacccgttt aataaggtat cggcaatcag tcattcgttt ttccttaaac aaggaatgca
      361 gccgttcaga ttagccctta catctcccca aaactgaacg tgcgagttat tgagggtgca
      421 tgctgcactc cacaccagag ctttgacgac accactcgtt tcaatggggg aattctgtgg
      481 catggtataa agcacagcaa aatcgccaat aacgaagcca attttaatgt acttccgaat
      541 tggcggtcac gttaatcttt ccatcaatat tgttttcttc gtaaaggctc gagtttttat
      601 gctaaagatt gcaagttgct tgtaaaagat aaccgccctg atccataatc gctcttgttg
      661 agggtgcgtg ctgcacaaaa ttaaagttgt aaagtaaacc ccctgttcct taccagttcg
      721 ggggttttac tttttaaaga gaacggtatt attttaactt tcaataatta ccgtggcgca
      781 agcatagtgg cgttcatctg ccagcgttac atgcatattt acaacgccga gcttttccgc
      841 cagttttaat gcctcgcccc acagtcgcag ccgtggctta ccaagctcat cgttaaatac
      901 ttcaaactga ttaaacgcca gaccattgcg gatcccggtg ccaaacgctt ttgctgcggc
      961 ttcttttacg gcaaagcgtt tcgccagaaa acgcaccggc tggtggtgcg ttttccaaat
     1021 ttcccattcg ttatcgctta atacgcggcg tgccaggcgc tcaccggatc gggtgatcac
     1081 cgcttcaatg cgagcgatct ccacaatatc cgtacctaaa cctaatattg ccattagcca
     1141 cgcgcttcca gcatcaaacg cttcatttct gccaccgcat ctttcagtcc ggtcatcact
     1201 gcacgaccaa taatggcatg accgatattc agttcatgca tctcagggat ggcggcaata
     1261 gctttcacgt tgtgataggt cagaccgtga ccagcgttaa ctttcagacc gagactagcg
     1321 gcaaaggtcg cggctttggc gatacgcgcc agctcttgcg cctgttcggc gtcagttttg
     1381 gcatcagcat agcaaccggt gtggatctcg ataaacggtg cgccaacctc tgccgcagct
     1441 ttgatctgcc cttcatcggc gtcgataaac agagaaacct ggatcccggc ctccgccaga
     1501 cgtttacagg catcgcgcat tttgtcacgc tgccctgcga cgtccaggcc gccttcggtt
     1561 gttacttcct gacgcttttc cggtaccaga cagcaaaaat gtggcttcgt ctcaacggcg
     1621 atcgccagca tctcttcggt caccgccatc tccagattca tgcgggtatc cagcgtctga
     1681 cgcaggatgc gcacatcacg gtcagtaatg tggcggcgat cttcacgtaa atgcacggta
     1741 atgccgtccg ctcccgcctg ctcagcaata aacgccgcct gcaccggatc cgggtaagca
     1801 gtaccgcgtg cgttacgcag cgttgcaata tggtcaatgt tgacgcctaa cagtaattca
     1861 gccatgacaa tcctcatcat tcataatgtg ttttcaccgt tcgcttaggc ataaactgcc
     1921 ggaacagttc cctgctcttt aatggtttac cgccaagata cggcttaagc gccatgcggg
     1981 taaagcgttt cgcggcgcgc agtgtgtctg cgtcaggaaa ttcccgcgcg tttaacgctt
     2041 ttaactgcct tccggtgaac gttttattgt cgataacgac gcttgcgata aacccttttt
     2101 cttcgcgata acgatacgtc atggtgtcat ctaccggctc gccgctaccc gcacaatggg
     2161 taaaattgac gccatagccc agatgcccga gcagtgccag ttcaaagcgg cgcagcgcgg
     2221 gttctggcgt accagtggca cctgcaagag actgaatgca gtgcaagtaa tcgaaaaaga
     2281 gttcagagaa gcgcgtctcg tattccagta cgcgggagag aagttcgttg atgtacagac
     2341 cgctgtaaag cgtgataccg cttaatggca gcgccagcga gacggcttca gcactgcgca
     2401 gcgttttgac ttcgccacgc ccgccgaaac gtagcaagag aggggtgaaa ggctgtaatg
     2461 cacctttcag agtagagcgt ttagagcgtg cgcctttggc aaccagacgc acgcgacccg
     2521 attcctccgt gaagacgtcc agcatcaggc tggtttcgct ccacgggcga ctatgcagga
     2581 caaatgcgcg ctgccagcct tccatcggag ttactcttaa agatcgtcaa cgtaaccgag
     2641 actgcgcagt gcgcgttcgt cgtcggccca accggatttc acttttaccc acagctcaag
     2701 gtgaacaggc gcttcgaaca tttcctgcat gtctttacgc gcttcaatcc cgatggtttt
     2761 gatcttggcc cctttgttgc caataaccat cttcttctgc ccttcacgct caacgagaat
     2821 caaaccgttg atgtcataac cgccgcgttc gttagagacg aaacgttcga tctccacggt
     2881 cacggagtac ggcagttcag cgccgaggaa acgcatcagt ttttcgcgga tgatttcaga
     2941 cgccataaaa cgctgtgagc gatcggtgat gtaatcttcc gggaagtgat gagtcgcttc
     3001 aggcagatgc ttacgcacga ttgccgcaat agtatcaaca ttcaacccgg tttcggcaga
     3061 gatcggcacg atatcgagga agttcatctg gcttgccagg aactgtaagt gtggcagcag
     3121 atcggctttt tcctgcacgt tatccacttt gttcaccgcg aggattaccg gcgctttgcc
     3181 atcgcgcagt ttgttgagca ccatttcatc gtccggcgtc cagcgggtgc cttctacgac
     3241 aaaaatcacc agctcaacgt cgccgataga gctgctcgcc gctttgttca tcaggcggtt
     3301 aatggcgcgt ttttcttcca tatgcaggcc tggggtatca acgtagatcg cctgatacgc
     3361 gccttcagta tggatcccca caatgcggtg acgagttgtt tgcgccttgc gggaggtaat
     3421 ggagatcttc tgccccagca gtttgttcaa caatgtggat ttgccaacgt tcggacgtcc
     3481 gacgatagca ataaatccgc agtaactttt atcgatgctc attccagctc cagttttttc
     3541 aacgcctgtt cggcggcagc ctgctcagcc ttacgacggc ttgaacctgt gccaaccacc
     3601 ggttcactca ggccgctgac ctggcagtgg atagtaaatt cctgatcgtg tgcttcgcca
     3661 cgtacctgga cgaccagata agtcggcagc ggcagatggc gaccctgcaa atattcttgc
     3721 aagcgcgttt tcggatcttt ttgtttgtca cctgggctaa tttcgtccag acgggtttga
     3781 taccagttaa ggattaattt ctcgacggtt tgaatatcac tgtcgaggaa tacgccacca
     3841 attaatgctt cgacggtgtc ggcgagaatt gactcacgac gaaatccacc gcttttaagt
     3901 tcacctggcc ctaaacgtaa gcactcgcct aactcaaatt cgcgcgccag ttctgccagc
     3961 gtattgccac ggaccagcgt ggcgcgcatc cggctcatat cgccttcatc cacacgaggg
     4021 aaacggtgat aaagcgcatt ggcgataacg tagctcagaa tagagtcgcc taaaaattct
     4081 aaacgctcgt tatgtttgct gctggcacta cgatgggtta atgcctgctg caacagttcc
     4141 tgatgattaa aagtgtagcc cagcttccgt tgaagccgat taattacgat ggggttcatg
     4201 cgataccaat aaataaatgc gtcaacaatt cagcacacga aacagacctg atatacatgg
     4261 ttctgctaac tgcttcgctg cagtttcagt ttaccggtat atggggacca acgctgtttc
     4321 gtgtgccgtg gcaacctgga ggtgccaagc ttaaacttcg ggggaatatt ctatacacaa
     4381 cgacggggga tgtcgttagc cacgggagat ttatctcata aataattcac gttgtcgcca
     4441 taacggcgac aacgtgaacg aagatgggct attaatggat gccgccaatg cgacttaagc
     4501 gcacacctgt cggccattcg ccttcttgct tatcgaagct catccagata gccgttgccc
     4561 gaccgaccag attcgcttcc ggcacaaagc cccagtaacg gctgtccgcg ctgttgtcgc
     4621 ggttgtcacc catcatgaag tattgtcccg gcggaacaat ccaggttgcc agttgttgcc
     4681 ctggctgctg gtaatacatc cccacctggt cctgcgcaat cggcactgtc agaatgcggt
     4741 gcgtcacatc acccagtgtc tctttacgct cggaaagacg aattccattt tctttggttt
     4801 cgtttttcgg cacttcaaag aatccgctgg tcgcttcccc accattacgg cgtgagaagg
     4861 tctgaacgaa atcgctcggt tccacgtttg agtaggtgac cggcagcgcg ttttcacatg
     4921 cctggccgga actacatccc ggttgaatcg tcagctcttt tgagaccgga tcataagtga
     4981 ctttatcgcc cggtaaaccc accgcgcgct tgatgtaatc aagctttgga tcttccggat
     5041 atttaaagac cacgatatcg ccgcgtttcg gatgaccggt ttcgatcagc gttttctggt
     5101 agataggatc tttaatgcca taagcaaact tctctaccag aataaaatca ccgattaaca
     5161 gagtcggcat catcgaacct gacgggatct ggaacggttc ataaataaac gaacgcacaa
     5221 tcaatacgat agccagtacc ggaaaaacag aagcaccggt ttccagccag ccaggcttcg
     5281 gcgcaacctt tttcaacgtt gctttatcca gtgagtcacc ggcagccgcc tgcgccgctg
     5341 cctgacgttc ccgccgttta ggtgcgaaaa agaatttatc cacgcaccat aaaatgcccg
     5401 tcaccagtgt ggcaatcacc agaatcaggg caaacatatt cgccatgcca actcctaagg
     5461 gttatttgtt gtctttgccg acgtgcagaa tggcgaggaa cgcttcctgc ggcagctcga
     5521 cgttaccgat ctgcttcatg cgtttcttac cttctttctg cttctgcagc agctttttct
     5581 tacggctgat atcgccgcca taacatttag ccagtacgtt tttacgcagc tgtttcacgg
     5641 tggatcgcgc aataatgtgc gtaccaatcg ctgcctgaat ggcgatatca aactgctggc
     5701 gtgggatcag atctttcatc ttctccacca actcgcgacc gcggttttgc gaattatcac
     5761 ggtgggtgat caacgccagc gcatcaacac gttcaccgtt gattaatacg tctacacgta
     5821 ccatgtcgga cgcctggaag cgtttgaagt tgtaatccag agacgcataa ccacgcgagg
     5881 tagatttcag gcgatcgaag aagtcgagca ccacttccgc catcgggatc tcgtacgtca
     5941 gcgccacctg attaccgtgg taaaccatat tggtctgcac gccgcgtttt tctacgcaca
     6001 gcgtaataac gttgccgaga tatgcctgcg gcagcagcat gtgacattct gcaatcggct
     6061 cgcgcagttc gtagatgtta tttaccgctg gcagcttgga tgggctgtcg acgtagataa
     6121 cttctcttga cgtggtttca acttcataca ctacggtcgg cgcagtggtg atcagatcca
     6181 gatcgtattc acgttccaga cgttcctgga tgatctccat gtgcagcaga ccgaggaagc
     6241 cgcagcggaa accaaagccc agcgcactgg agctttccgg ctcatagaac agtgaagcat
     6301 cgttcaggct gagtttgccc agcgcgtcac ggaaggcttc atagtcgtcg gaacttaccg
     6361 ggaacagacc ggcgtatacc tgcggtttga ctttcttaaa gccaggcagc gccttttctg
     6421 ccggattacg cgccagcgtt aaggtatcgc ctactggagc gccgtggata tctttaatcg
     6481 cacataccag ccagcctact tcgccacatt tcagttcagt gcggtcaacc tgcttcggcg
     6541 tgaagatgcc cagacggtcg gcgttatagg tctgcccggt actcatgact ttcactttgt
     6601 cgcccttacg cagggtgccg tttttaatac ggataagtga aacaacgccc aggtagttgt
     6661 cgaaccagga gtcgataatg agtgcctgta acgggccttc cggatcgcct tccggcggcg
     6721 gaatgtcgcg caccagacgt tcgagaacgt cctgcacgcc aacgccggtt ttcgctgaac
     6781 agcgcaccgc gtcggtggca tcgatgccga caatatcttc aatttcttcc gccacgcgtt
     6841 caggatcggc tgccggcagg tcaatcttgt tcagtaccgg caccacttcg agatccattt
     6901 ccatggcggt gtagcagttt gccagggttt gcgcttctac gccctgcccg gcgtcgacca
     6961 ccagcaatgc accttcacag gcagccagcg aacgagaaac ttcataagag aagtcaacgt
     7021 ggcccggggt gtcgataaag ttaagctgat aggtttcgcc gtcagacgct ttgtagtcca
     7081 gcgtcacgct ttgcgctttg atggtaatgc cacgctcacg ctcaagatcc atggaatcga
     7141 gaacctgcgc ctccatttca cggtcagaca ggccaccgca gatctggata atacggtcag
     7201 acagcgtcga tttaccgtgg tcaatgtgag ctatgatcga aaagttacgt atattcttca
     7261 taaagtatga ttattgtgcc ttaatgcccg gttaaccagg cttttagaag tcgctgttct
     7321 gagcttaacg tctgtattaa tagaaacgcc gcattctaca ctacaacatt gaggcgagga
     7381 aatgttcata ccgtatggat tgtggtatct ggaaacgtcc tcgcatttgt tatgcaaaat
     7441 gcaacaaagc cagtgaaatc actggctcgc gtcttccgaa gatgtttcaa atcgcacaag
     7501 gccaggcggc aaggccacgc ttaagatgat cggttgccat tcagaccggg cagcaaactt
     7561 tcgagagcag ccacgtgcaa tcaggaatcc accaacgcca ccgagaatcg caccgcataa
     7621 tgctgcaata tctgaagcaa agagtagctg aaatagtgaa gcgatgagga atagtcccat
     7681 caacggcgac atataaacca gtaatgcgga gctaagcagg ctgccttcgg cgattcctaa
     7741 ttctactttt tgccccggca ctaacggctc atcacagggt acgacaatgg tatgcgtggt
     7801 ttgcgggcca agtttattca acacgcggct accgcaaccg gcgcgtgaag cgcagctgct
     7861 gcatgaggct ttaacatcac aactaaccag cgcctgcccg ttttcccagg agatgacggt
     7921 agcccactct ttgatcattg cgctgccccg aacttaatat tctcggcaat gcgtttcgcc
     7981 gtttgcggcg gcagttcacc gacaatggtg atttcggcgt tatcacgtac gcttgtactg
     8041 acggttctgc gtccggtgcg caacatctga tcggtgctcg atggcgtagc gcggttaacg
     8101 tttaccgaaa agctgaataa tccgtcggaa tagagacgtg attcgatagg catgttgtcc
     8161 atcgtcggta gcggacgtcg actactggaa acttcgctaa aaccctgtgg caaccaggtt
     8221 ggcgtccagc tgaatttagc tttttcacct acaggaacag aaagcaacgg cggcaaattt
     8281 gccttcgcca acgtctgcat actgctgctg atatcctgat tgacattaaa agcaatcacg
     8341 cgaaattgtt ccagcgtttc accatcgcga tcaaggagat caacccgcat cggtaatttc
     8401 gattcggtgt ccatccacac gatgtagctg tagcgtgtac catctcgggc aaccacacga
     8461 atgacttcgc aaagacgatc agcaatacgc gtgcgcccga cggagataaa gtcgtagtaa
     8521 ggagaaaggc gtttgaaatc ggtatagatg agcgatggta gagaatcaac aatgtaatcg
     8581 ccattaagcg tgaacggttc aagtcccggt tcaaaatagc tgatttcatt gccgcgctgt
     8641 accacttccc ggcgcgggcc atccatttgc aacaattgtg caagaggacg gttatcgagg
     8701 cgtgcatgtc gataacgcag agactcaaca ccctgtttat tgatgctgat gaatgacagc
     8761 tcgtaattca gtgactgact ggccaggttc atctgctgta ataacgcccc ggacgcggga
     8821 gtggccgagg cgttagcaga gaataacagg ctacctgtca ctaatgacat ggcaaaccaa
     8881 agttgcttca ttactgcgat tgcgttccta aagtttgaat tcctggcacc tgtacagcgg
     8941 cttgctgagt ttgcgcctgc tcaaactgaa gctgttcaga gtggagtcgg cgttgcagtt
     9001 cgtaatcctg caacattgca ttaatgcgac gacgctgctc ctgtacctgc tgctgttgac
     9061 cattatttgc ggtcgcttca gaaggtactc ccaagctaac cgggctggct ttacccatca
     9121 tcggcagtgt attaaatacc ggcgtttcgg gctgctggga cgtttcagat tgtccattat
     9181 agtgctggac cccaacgata actgcaagcg atacgcatgc tgctacgccc atttgggtaa
     9241 gctgtgccgc ccacggacgt actttctgcc agaatggcat tttctgccat tgatgcggcg
     9301 caggctgggc ttccgggatc aatgtcgccg gttgacgtac tggctcttct tcaatggcgg
     9361 ccatcacgcg tgaagagata tcgaaatgga gcacctcggg agtatcaccc cgcattgagt
     9421 cacggattaa gtgatagctt tcccaggttt tctgcatttc tgggttatga gccagttcgt
     9481 taagcagctc actatccagc gtttcgccat ccattaaagc ggaaagttgt tctttctgca
     9541 tgcctaatac ccttatccag tatcccgcta tcgtcaacgc ctgataagcg gttgaacttt
     9601 gttatcaata gcttccctcg ctcggaagat acgtgaacgc accgtaccta ccggacaatc
     9661 catgatagcg gctatctctt catagctcag gccatccagc tcccgcaagg ttattgccat
     9721 gcgtaaatct tccgggaggg actcaatagt tcggaaaact atctgtctca gttcttctga
     9781 caacattaag ttctcagggt tcgaaatttc tttcaacgcg ccgccacttt cgaagttttc
     9841 agcttcaatg gcatccacat cactggaagg tggacgacgc ccctgagcaa ccaggtaatt
     9901 tttcgctgta tttacagcaa tccgatacag ccatgtataa aaagcgctat ctccccggaa
     9961 cgaatccagc gcacgatagg ctttaataaa agcttcttgt accacatcgg gaacatcacc
    10021 cgacggcaca tagcgggaaa ccagactcgc cactttatgc tgatagcgca ctaccagtaa
    10081 gttaaaggct ttctgatctc ccttctggac ccgttcaacc aggacctggt ccgttaactg
    10141 ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc acgcgctatc gaaacgccac
    10201 tccattagct gcaatttgag caagcaaagg gttagagtgt ctcgtttttg taaagttccg
    10261 taacgcatct gtttttgttt gtcatgctgt agacggatca ttatctatca ttataagtct
    10321 acagaatctg aacatcgcat tatctgtgta gaaatgccca tttaactgcc tgaagagtaa
    10381 cccaacggcc tttttatttc accacctaat cctccaccag ccagtaactt ctctttttct
    10441 cgccgccctg cgtcagcgtg tttagccact gtaacaaata ttaaaatagc aggtgtttaa
    10501 tcgcacaaca tgatgctatg ctgaccaaac cgtgtttagt aaattaaaca aagaaaatga
    10561 atactctccc tgatcattca tgtgacgtgt tgattatcgg tagcggcgca gccggacttt
    10621 cactggcgct gcgcctggct gatcagcatc aggtcatcgt tctaagtaaa ggcccggtaa
    10681 cggaaggttc aacattttat gcccagggcg gtattgccgc cgtgtttgat gaaactgaca
    10741 gcattgactc gcatgtggaa gacacattga ttgccggggg tggtatttgc gatcgccatg
    10801 cagttgaatt tgtcgccagc aatgcacgat cctgtgtgca atggctaatc gaccaggggg
    10861 tgttgtttga tacccacatt cagccaaatg gcgcagaaag ctaccatctg acccgtgaag
    10921 gtggacatag tcaccgtcgt attcttcatg ccgccgacgc caccggtaga gaagtagaaa
    10981 ccacgctggt gagcaaggcg ctgaaccatc cgaatatttg cgtgctggag cgcagcaacg
    11041 cggttgatct aattgtctct gacaaaattg gcctgccggg cacgcgacga gttgtaggcg
    11101 cgtgggtatg gaaccgaaat aaagaaacgg ttgaaacctg ccacgcaaaa gcggtggtgc
    11161 tggcaaccgg cggtgcgtcg aaggtttatc agtacaccac caatccggat atttcttctg
    11221 gcgatggcat tgctatggcg tggcgcgcag gctgccgggt tgccaatctc gaatttaatc
    11281 agttccaccc taccgcgctg tatcacccac aggcacgcaa tttcctgtta acagaagcac
    11341 tgcgcggcga aggcgcttat ctcaagcgcc cggacggtac gcgttttatg cccgattttg
    11401 atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac catgaaatga
    11461 aacgcctcgg cgcggattgt atgtatctcg acatcagcca taagcctgcc gattttattc
    11521 gccagcattt cccgatgatc tatgaaaaat tgctcgggct ggggattgat ctcacacaag
    11581 aaccggtacc gattgtgcct gctgcacact atacctgcgg tggtgtgatg gtggatgatc
    11641 atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat accggcttac
    11701 acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat ggctggtcgg
    11761 cggcagaaga tatcaccaga cgtatgcctt atgcccacgg cgtcagtacg ttaccaccgt
    11821 gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat aactggcacg
    11881 agctacgcct gtttatgtgg gattacgtcg gcattgtgcg cacaacgaag cgccttgaac
    11941 gcgccctgcg gcggataacc atgctccaac aagaaataga cgaatattac gcccatttcc
    12001 gcgtctcaaa taatttgctg gagctgcgta atctggtaca ggttgccgag ttgattgttc
    12061 gctgtgcaat gatgcgtaaa gagagtcggg ggttgcattt cacgctggat tatccggaac
    12121 tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata aacagataaa
    12181 aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca cgaatgacta
    12241 agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc cgatgcggca
    12301 gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc atacttaatg
    12361 ccagttcagt aaaaccatta ccaatctgct ctggcagcac cacgcagaaa aatccctctt
    12421 cggtaatgca ctccgccgca caggtcagca acgatgggtg atcaagcgta gtggtatagc
    12481 gagcctgttc ccgttgaggt gtcgagcact ctactccctg ctgatagtaa ggtgggttac
    12541 tgatgattaa atcgaagcgt actgtctgct gtgtgatcca ctgctgaata tccgccgtat
    12601 ggacgttaat ccgctttgcc cacggggact ggttgatatt ttcctgtgcc tgcgcggcag
    12661 cttcactttc cagttcaact gcatcaatca ttacgctgtc atcggttcgc tgcgccagca
    12721 ttaatgccag caacccgcta cccgcgccga tatcaaggca acgttttacc ccagccaccg
    12781 gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacaa cgatcgtgag
    12841 caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta gactgtgaca
    12901 tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa tacccgagaa
    12961 gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca gcgccccaca
    13021 cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga aagcctgctg
    13081 gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc cgccattccg
    13141 cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg taaaacggcg
    13201 gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cacgtaagaa atccggtccg
    13261 ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc cgatcatgcc
    13321 cgcgaactgg cgaaacatac gcatctggat attgccacca tcaccggcgg cgtagcctat
    13381 atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac gaccggacgt
    13441 ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac gctgatcctc
    13501 gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca tattgctggc
    13561 gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa ctctggaagg cgatgcgatt
    13621 caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa tccgtccaca
    13681 cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga acataaaacc
    13741 gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt gtttgtgcgt
    13801 aagcgtgagc gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat caacaactgc
    13861 tatctcgaag gtgagatggt acagggcaag cgtaacgaag cgatcaagcg tttgaccgaa
    13921 ggtcgcgtaa acgtactggt tgccaccgat gttgccgcgc gcggtatcga cattcctgac
    13981 gtcagccacg tctttaactt cgatatgccg cgcagtggcg atacttattt gcaccgtatc
    14041 ggacgtaccg cgcgcgccgg tcgtaaaggc accgcaattt cgctggtaga agcccatgac
    14101 catctgctgc tgggcaaagt tggtcgctat attgaagagc caattaaagc tcgtgtaatt
    14161 gatgagttac gcccgaaaac gcgtgcgcca agcgagaagc agaccggcaa gccatcgaag
    14221 aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa gccgcgggtg
    14281 aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccgag cggaacgggc
    14341 gtgccaccgc aaacgacaga agagtaatct caatgccggg tttaagcccg gcattatttt
    14401 tatcccggca aaaaacactc ttcaacacac tgaataaaaa tgtgcatcgc cgggctaacc
    14461 gcttttccgg catggtgagc gcacattgcc gtaatggtct gcgactgttc gccaaaaggc
    14521 aattcgatta attcgccact cgcaagttca tttgccactg taaagcgcgg caggtaactg
    14581 accccgatat ttgccgcgac acaacgcttg atgctttcga tacttataag ctcaatggtg
    14641 ttttccaccg tgatccgtcg ctggcgcagc gtactttcaa atagctggcg aaagacacat
    14701 tgcggttcat tgataataaa actacaggcg ttatgtcttc ccggctcagt aaaatcgaca
    14761 tctttaattt gcggtgaagc caccagcacc agtgattgtt cacccaactc tcgtcggttc
    14821 agagcatcat cattccctac gcggtaaaag acgccgacat cagcctcatc attcagtagc
    14881 gcatcacgga tcacgtaaca gttcagagac tgcaacgata aacgcacttt tggtgcgcgt
    14941 tgccgaaaac gctgcaacac ctgcggcatt cggtaagaaa gcaacgtttc gcctgaaaca
    15001 acgcgtagtt ccccgtccgg atccgactct tttttggcag cctcacgaag ggtatccatc
    15061 actctggtga gttcatagat gtgcggcagc agcttttttc cttcgcgggt aaggcacatt
    15121 cgtcgaccaa ttttctcaaa taactggact gagaactcct gctcaagctg ctgaatatga
    15181 aaagtcaccg tcgattgtgt gcagcacaat ttttgcgaag ctcgcaaaaa ggacccctct
    15241 tccaccacgg ttttaagcgt aataaaacgg cgcagatcca taaccccaaa cctatcgaaa
    15301 atatcgaatt tagaatgtaa aaacattcat ttttttaaat gttccgtgtc gggtactgtc
    15361 taccaaaaca gaggagataa caagtgacac cgaccctttt aagtgctttt tggacttaca
    15421 ccctgattac cgccatgacg ccaggaccga acaatattct cgcccttagc tcagctacaa
    15481 cgcacggatt tcgtcagagt actcgcgtgc tggcagggat gagtctggga tttttaatcg
    15541 tcatgttgct atgcgtggga atttcgtttt cactggctgt tatcgacccg gcagcagtgc
    15601 atctgttgag ctgggcagga gcggcatata ttgtctggct ggcatggaaa atcgctacca
    15661 gtccaacaaa ggaagacgga cttcaggcaa aaccaatcag cttttgggcc agctttgctt
    15721 tgcagtttgt gaacgtcaaa atcattttgt atggcgttac ggcactgtcg acgtttgtcc
    15781 tgccgcaaac gcaggcgcta agctgggtag ttggcgtcag cgttttgctg gcgatgattg
    15841 ggacgtttgg caatgtgtgc tgggcgctgg cggggcatct gtttcagcga ttgtttcgcc
    15901 agtatggtcg cccgttaaat atcgtgctgg cgctgttgct ggtctattgt gcggtacgca
    15961 ttttctattg aaagacaaaa agaaaagcgg aagaggtcac cctcttccgc ttaataactt
    16021 gctacttaag ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc
    16081 cggagtcaga gagttaaagc gaactgcata accggataca cggatggtca gctgcggata
    16141 tttttccgga tgcttaactg catcttccag agtttcacga cgcagaacgt taacgttcag
    16201 gtgttgacca ccttcaacgc gaacttctgg tttcacttct actggaactt cacggtattc
    16261 aatatcaccc agtttgctta ctgcaaccac ttcgtcttct gcaaaacctg ctttcgcaac
    16321 gatgcaacgc gcttcgcctt tttcgctgtc cagcagccag aaagagttca gcagatcgtc
    16381 gttagcggct ttagtaatct ggatacctgt aatcatgtga tgcctccccg gcaaaattat
    16441 ttgatttgtt cagcctgtcg cggccaattg gtaaaaccat tgttgcttga gtgtatatat
    16501 actcctcaaa cacccttgaa tctttgattt aaatcaataa aaaccacaca tcaagtatgg
    16561 ttgcaaatgg attttattgt tttacatcaa cttatgcagg tgtgaaattt taccaattta
    16621 catttttttg cactcgttta agtctaaaaa atgagcatga ttttgttctg tagaaagaag
    16681 cagttaagct aggcggattg aagattcgcg ggagagcgag atggcaaacg aattaacctg
    16741 gcatgacgtg ctggctgaag agaagcaaca accctatttt cttaataccc tacagaccgt
    16801 cgccagcgag cggcagtccg gcgtcactat ctatccgcca caaaaggatg tctttaacgc
    16861 gttccgcttt acagagttgg gtgacgttaa agtggtgatt ctcggccagg atccttatca
    16921 cggaccggga caggcgcatg gtctggcatt ttccgttcgc cccggcattg cgacaccgcc
    16981 gtcactgctg aatatgtata aagagctgga aaatactatt cccggtttca cccgccctaa
    17041 tcatggttat cttgaaagct gggcgcgtca gggcgttctg ctactcaata ctgtgttgac
    17101 ggttcgcgca ggtcaggcgc attcccacgc cagcctcggc tgggagacct tcaccgataa
    17161 agtcatcagc ctgattaacc agcatcgcga aggcgtggtg tttttgttgt ggggctcaca
    17221 tgcgcaaaag aaaggggcga ttattgataa gcaacgccat cacgtactga aagcgccgca
    17281 tccatcaccg ctttctgcgc atcgtgggtt ctttggctgc aaccattttg tgctggcaaa
    17341 tcagtggctg gaacaacgtg gcgagaaacc gattgactgg atgccagtat taccggcaga
    17401 gagtgagtaa atttgcagga aaacgccgga tggcagcgtt gccacccggc tgatttatca
    17461 ggctttattc tgacgccacc attcaccaag caaaacgcca gttgcgacag agatatttag
    17521 cccggcaacg ttgccagtac cgtcaatctt cacgcgcaga tcgtttggat cgcgtgcggc
    17581 atccggtaac ccttcatatt cctgacccag caccagtacc attttcgctg gcagactggt
    17641 tttgaacagc ggtttcccct gctcgctgga agtggtcact acggtgtaac ctgcctgacg
    17701 gaaatcatcc agcacgttaa caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc
    17761 gccttctgcg gtacggatag ccgcccccga ctccagcagt gcctcatcct gcaccacaac
    17821 gcctttcacg ccgaagtgcg cacagctgcg catcatgcca cccaggttat gcgggttaga
    17881 ttcgttttcc agtgccagaa cgcaatcctg cgcgcctgcc tggcttaccc actgctgcac
    17941 ggttgtaccg ttacgctttt tgatcaggaa gcaaacgccg ccgtgatgtt ccgtgcctga
    18001 cgcttttgtc agttccgctt catctaccac atggtacgct ttgcggtttg ctgccatcca
    18061 gcgcaaggct tctttaaaac gcggcgttac actctggata aaccaggcgc gaacaatcgc
    18121 ttccggacgg ctctggaaca gtgcctgaca ggcattttcg ccgtagacgc gggtttcttc
    18181 cgcacgctga cgacgcaaaa cttccggatc aataaaactt ttaccactga tgccaccgtg
    18241 atcggccttt tccggcgtct catcacccgg cgcgcgggaa accgtgcgcc acggcgaatc
    18301 ttcccacttg cggtcacggg ggtgactctg tttgtcatcg cgggcggggc gacggccacc
    18361 gtcagcacga gattttcctg gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg
    18421 tttatcagaa tcatcatcac tgcggacata catcactttg accttgccgc ttttaccttt
    18481 catttcatcg ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga ttacccgaag
    18541 tccgcgcggt tcgccatgat ttcgtaccaa agcctgcgac tatcatacct attgaataaa
    18601 acagattgtt gtctggaaca atgtccccga taatatgtaa catattagaa acataccggc
    18661 gtcgttgccg ataagtctcc ttactcatcc cgaggttagt tatgaatacc gtttgtaccc
    18721 attgtcaggc catcaatcgc attcccgacg atcggatcga agatgcggca aaatgcggac
    18781 gctgcggtca cgacttgttt gacggagagg tgattaatgc gaccggtgaa acgctcgaca
    18841 aattgctgaa agatgatcta cctgtggtga tcgacttctg ggcaccgtgg tgcggcccct
    18901 gccgtaattt cgcaccaatt tttgaagatg tcgcgcaaga gcgtagcggt aaagtgcgct
    18961 ttgtgaaagt gaataccgaa gctgaacgtg aattgagcag tcgctttgga attcgtagta
    19021 taccgacgat catgattttc aaaaacggtc aggttgtcga catgcttaat ggcgcagtac
    19081 cgaaagcgcc gtttgatagc tggctgaacg aatctcttta atcttaccgg ggcgcatctt
    19141 gtgccccgtt ttctcctctg cgacaatggc gttttttcga cgctctctta tgaccgaaaa
    19201 cgctgttctc cagttacgcg ccgagcgtat tgcgcgcgca acacgtcctt ttcttgcccg
    19261 cggtaatcgc gttcgtcgct gccaacgctg tcttttgcca gagaaattat gtctctgttc
    19321 gacaattacc ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc
    19381 aatgaagccc agtaataccg ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt
    19441 tcaatggtcg cgtaccgaac cctcgctaga tttgctggat ctggtacaaa acccggacta
    19501 tcagccaatg gtggtctttc ccgcctcgta tgctgatgag caacgggaag tgatcttcac
    19561 accacctgcc ggtaagcctc cgctatttat catgctcgat ggtacctggc ctgaagctcg
    19621 caagatgttt cgtaaaagtc cgtatctgga taatcttccc gtcatttccg tcgatctttc
    19681 ccggctttct gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga
    19741 ggtagccatc gcactgttag atatggccgg cgataccggg gcggcggcag ggttaggcga
    19801 gcattttacc cgctttaaaa cacgctatct ggcaggaaaa acgcaacatc tgggtagcat
    19861 cacagcagaa cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg gagaccggta
    19921 tgagtcagcg aggactggaa gcactactgc gaccaaaatc gattgcggta attggcgcat
    19981 cgatgaaacc caatcgcgca ggttacctga tgatgcgtaa cctgctggcg ggaggcttta
    20041 acggaccggt actcccggtg acgcctgcct ggaaagcggt gttgggtgtg ttggcctggc
    20101 cggatattgc cagcttgccc tttacacccg accttgcggt tttatgtacc aatgccagcc
    20161 gtaatcttgc tcttctggaa gagctcggcg agaaaggctg taaaacctgc attattcttt
    20221 ccgccccggc atcgcaacac gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc
    20281 tgctaggacc aaacagtctg ggtttactgg ctccctggca aggtctaaat gccagctttt
    20341 cacctgtgcc gattaaacgc ggcaaactgg cgtttatttc gcaatcggct gccgtctcca
    20401 acaccatcct cgactgggcg caacagcgtg agatgggctt ttcctacttt attgcgctcg
    20461 gcgacagcct ggatatcgac gttgatgaat tgctcgacta tctggcgcgc gacagtaaaa
    20521 ccagcgccat cctgctctat ctcgaacagt taagcgacgc gcgacgcttt gtttcggcgg
    20581 cccgtagtgc ctctcgcaat aaaccgattc tggtgattaa aagcggacgt agcccggcgg
    20641 ctcaacgtct gttgaacacc acggcaggaa tggatccggc gtgggatgcg gctattcagc
    20701 gtgccggttt gttgcgggta caggatactc acgagctgtt ttcggcagtg gaaaccctca
    20761 gccatatgcg cccactgcgt ggcgaccggc tgatgattat cagcaacggt gctgcgcctg
    20821 ccgcgctggc gctggatgcc ttatggtcac gcaatggcaa gctggcaacg ctaagcgaag
    20881 aaacctgcca gaaactgcgc gatgcactgc cagaacatgt ggcaatctct aacccgctcg
    20941 atctacgcga tgacgccagc agcgagcatt atgtcaaaac gctggacatt ctgctccaca
    21001 gccaggattt tgatgcgctg atggttattc attcgcccag cgccgctgct cccgcaacag
    21061 aaagcgcgca agtattaatt gaagcggtaa agcatcatcc ccgcagcaag tatgtttctc
    21121 tgctgacgaa ctggtgtggc gaacattcct cgcaagaggc acgacgatta ttcagcgaag
    21181 ccgggctgcc gacctaccgt accccggaag gaaccatcac tgcttttatg catatggtgg
    21241 agtaccggcg taatcagaag caactacgcg aaacgccggc gttgcccagc aatctgacct
    21301 ccaataccgc agaagcgcat cttctgttgc aacaggcgat tgccgaagga gcaacgtcac
    21361 tcgataccca tgaagttcag cccatcctgc aagcatatgg catgaacacg ctccccacct
    21421 ggattgccag cgatagcacc gaagcggtgc atattgccga acagattggt tatccggtgg
    21481 cgctgaaatt gcgttcgccg gatattccac ataaatcgga agttcagggc gtaatgctct
    21541 acctgcgtac agctaatgaa gtccagcaag cggcaaacgc tattttcgat cgcgtaaaaa
    21601 tggcctggcc gcaggcgcgg atccacggcc tgttggtgca aagtatggct aaccgtgctg
    21661 gcgctcagga gttgcgggtt gtggttgagc acgatccggt tttcgggccg ttgatcatgc
    21721 tgggcgaagg cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgca ctaccgccgc
    21781 tgaacatgaa cctggcccgc tatctggtta ttcaggggat caaaagtaaa aagattcgtg
    21841 cgcgcagtgc gctacgccca ttagatgttg caggcttgag ccagcttctg gtgcaggttt
    21901 ctaacttgat tgtcgattgc ccggaaattc agcgtctgga tattcatcct ttgctggctt
    21961 ctggcagtga atttaccgcg ctggatgtca cgctggatat cgcgccgttt gaaggcgata
    22021 acgagagtcg gctggcagtg cgcccttatc cgcatcagct ggaagaatgg gtagaattga
    22081 aaaacggtga acgctgcttg ttccgcccga ttttgccaga agatgagcca caacttcagc
    22141 aattcatttc gcgagtcacc aaagaagatc tttattaccg ctactttagc gagatcaacg
    22201 aatttaccca tgaagattta gccaacatga cgcagatcga ctacgatcgg gaaatggcat
    22261 ttgtagcggt acgacgtatt gatcaaacgg aagagatcct tggcgtcacg cgtgcgatct
    22321 ccgaccctga taacatcgat gccgaatttg ccgtgctggt tcgctcggat ctcaaagggt
    22381 taggcttagg tcgacgctta atggaaaagt tgattaccta tacgcgagat cacggactgc
    22441 aacgtctgaa tggtattacg atgccaaaca atcgtggcat ggtggcgctg gcccgcaagc
    22501 tcgggtttaa cgttgatatc cagctcgaag aggggatcgt tgggcttacg ctaaatcttg
    22561 cccagcgcga ggaatcatga gtaaggtact ggaaatgttg accacttaat cgggactggt
    22621 gttattattg cccgcttatg tcgtctgcat tgcacagagg acccttcaat gaacagagaa
    22681 gaaatgcact gtgatgttgt caaaatttaa gcgtaataaa catcaacaac accttgccca
    22741 actacccaag atttctcaat cagttgatga tgtcgatttc ttttacgctc ccgccgactt
    22801 ccgggagacg ctgctggaaa aaatagccag cgcgaagcag cgcatttgca ttgtcgccct
    22861 gtatctcgaa caggatgacg gtggcaaagg cattctgaac gcgttgtatg aggctaaaag
    22921 gcagcgtccg gaactggatg tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg
    22981 cattggcgct gcggcatcta acactaacgc tgactggtac tgccgcatgg cgcaggaaaa
    23041 tccgggcgta gatgttccgg tttatggcgt tccaatcaat acccgtgaag cccttggtgt
    23101 tctgcacttt aaaggcttta tcatcgacga tagcgtactt tatagcggtg ccagcctgaa
    23161 cgatgtttac ctgcatcagc acgataaata tcgctacgac cgttatcatc ttatccgtaa
    23221 ccgtaagatg tcagacatta tgtttgaatg ggttacacag aatattatga atggccgcgg
    23281 cgttaatcgt ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg
    23341 tctgttccgc caggagctgc gtgatgccgc ttatcatttc cagggcgatg ccgacaacga
    23401 tcagctttcc gtaacgccgt tggtaggtct ggggaaatcg agtctgttga acaagaccat
    23461 tttccatctt atgccttgtg ccgagcagaa actaaccatc tgcacacctt acttcaacct
    23521 gccagcaatc cttgtgcgca atattatcca gttgctgcgc gaagggaaaa aggtcgaaat
    23581 tattgttggt gataaaaccg cgaatgactt ctacattccg gaagatgaac ctttcaagat
    23641 aattggcgca ttgccttatc tctatgagat caatctgcgt cgtttcctga gccgtttgca
    23701 gtattacgtc aacactgacc agttagtggt tcggttatgg aaagatgacg acaacaccta
    23761 tcacctgaaa gggatgtggg ttgatgataa gtggatgttg atcactggta ataacctgaa
    23821 cccgcgcgcc tggcgtctgg atctggaaaa cgctattttg atccacgatc cgcaactgga
    23881 gcttgcgcca cagcgagaga aagaactgga gctgatccgc gagcatacca ccatcgttaa
    23941 gcactatcgc gacctgcaaa gtattgccga ttatccggtg aaggttcgta aactcatccg
    24001 tcgtttgcgc cgtatccgca tcgaccgatt aattagccgc atcctgtaat cacaaccccg
    24061 tcctgtacgg ggtttgtttt ttggaggcca cgttttgcgt attctttttg tctgttcgct
    24121 attattactt tctggatgca gccatatggc taacgatagc tggagcgggc aggataaagc
    24181 tcaacacttt atcgcctcgg cgatgctttc cgccgccgga aatgaatatg cacagcatca
    24241 ggggatgagt cgcgatcgca gtgccacttt cgggctgatg ttctctgttg ggctgggggc
    24301 gtcaaaagaa ctttgggata gccgccccga ggggagcggc tggagctgga aagattttac
    24361 ctgggatgtc gccggtgcaa ctactggcta taccgtctgg caactgaccc gtcactaaag
    24421 acgcatcccc ttccctttgc gatgtagcat caaagaaacc agaaacgcca ccacggccat
    24481 caaggtcaca taccagaaga aggctgtttc cattcctatt gatttcagcg acaacgctac
    24541 gtactccgcc gaaccaccaa atatagcatt ggcgaccgca tatgacagac caacacctaa
    24601 tgcacgaacc tgtgccggga acatctcagc cttcagtatt ccactgattg aagtataaaa
    24661 actcacgatc agcagggcac acatcaccag accaaaagcg gcataaggcg aggaaacgtt
    24721 ttgcaatgct gagagaatag gaacggtaaa aatggctgcc agagaaccga aacataacat
    24781 tgaggtacga cgaccaatct tatccgacag cgcgccaatg agtggttgaa taagcatgaa
    24841 tacaaacaat gcggcagtca taatgccact cgccacgttg gcatgcattc ccgcagtatt
    24901 taccagatac ttctgcatat aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt
    24961 aaaaccgaga accatgatga atgcacggcg attgcgccat aatcctttca gagatccagc
    25021 ttcttttaaa gcgcgtgttt cttgttgtga agtttcatct aactgacgac gtaaccacaa
    25081 cgccacaaca gctaacacag ctcctaacgc gaaaggaata cgccatcccc actctctgag
    25141 tgcagcgtct tccatggtgt gttgtaaaac cacgacaacc agtagagcta gcagttgccc
    25201 gccgatcaac gtcacatact gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac
    25261 ttcactcata taggtggcgc tggtgccata ttctccgcca acggataatc cctggaataa
    25321 acgagcaaga agcaataatg ccggagccca ggtaccgatt gtttcgtagc ctggcaaaca
    25381 ggcgataacc agtgagccga aacacatcat acacaccgat aacagcatcg attttttgcg
    25441 accatgttta tcggctatgc ggccaaatag ccaaccgcct attgggcgca tcagaaatcc
    25501 cgcagcaaaa acacctgctg tttgcagtag ttgagtcgtc gtgttcccgg aagggaagaa
    25561 gatgtgagcg aagtagagtg aacagaacga gtagacatag aaatcgaacc actcgaccag
    25621 attacctgaa gaggccccca caattgccca aatgcggcga cgagtatcac tacttgtcag
    25681 tttgctgtct gccgttacag tactttcagc catgccatta tgtctcctgc cgtaatccga
    25741 tgcttttgtc ggtcgctttt gtttatttct tttggtaaag aaaatgttat acatttgttg
    25801 catatcatca tgcaacctta accacgaatt tagttagcag gaaaatggtt attgaggagc
    25861 ttaaggataa atttctggta aggaggacac gtatggaggt gggcaagttg gggaagccgt
    25921 attcgctggc gtatgtggag gaataagctg ccccctatcg tgcctgcgta agctggataa
    25981 gacgcgccat gaactgaacc ccaaaacctg gacagtttca gttaaccggc tttttgatac
    26041 tcagtccggt attctaccgg gctgagtccc ccaagactga gttttattct tttttgattc
    26101 cagtaagtta tgtattcgtt aacggcattt tccag
//
LOCUS       DARNVM010000059        26069 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.059, whole genome shotgun sequence.
ACCESSION   DARNVM010000059 DARNVM010000000
VERSION     DARNVM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.059"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1843)
                     /gene="espL1"
                     /locus_tag="SI621_002192"
     CDS             complement(<1..1843)
                     /gene="espL1"
                     /locus_tag="SI621_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HEI4064911.1"
                     /translation="MSENDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADMYVRNCAIAFLPL
                     LPEGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLIALFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPITQELLSKKMVMDILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAYGTPALYIAMSKGNKDVVLSYISTL
                     GTFANKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAI"
     gene            2366..4294
                     /gene="thrS"
                     /locus_tag="SI621_002193"
     CDS             2366..4294
                     /gene="thrS"
                     /locus_tag="SI621_002193"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEI4064912.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4298..4840
                     /gene="infC"
                     /locus_tag="SI621_002194"
     CDS             4298..4840
                     /gene="infC"
                     /locus_tag="SI621_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEI4064913.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4937..5134
                     /gene="rpmI"
                     /locus_tag="SI621_002195"
     CDS             4937..5134
                     /gene="rpmI"
                     /locus_tag="SI621_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEI4064914.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5188..5544
                     /gene="rplT"
                     /locus_tag="SI621_002196"
     CDS             5188..5544
                     /gene="rplT"
                     /locus_tag="SI621_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEI4064915.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5661..5785
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5667..5711
                     /gene="pheM"
                     /locus_tag="SI621_002197"
     CDS             5667..5711
                     /gene="pheM"
                     /locus_tag="SI621_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HEI4064916.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5850..6833
                     /gene="pheS"
                     /locus_tag="SI621_002198"
     CDS             5850..6833
                     /gene="pheS"
                     /locus_tag="SI621_002198"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEI4064917.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGNWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6848..9235
                     /gene="pheT"
                     /locus_tag="SI621_002199"
     CDS             6848..9235
                     /gene="pheT"
                     /locus_tag="SI621_002199"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEI4064918.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9240..9539
                     /gene="ihfA"
                     /locus_tag="SI621_002200"
     CDS             9240..9539
                     /gene="ihfA"
                     /locus_tag="SI621_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEI4064919.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9640..10620
                     /gene="btuC"
                     /locus_tag="SI621_002201"
     CDS             9640..10620
                     /gene="btuC"
                     /locus_tag="SI621_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HEI4064920.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWS
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10683..11234
                     /gene="btuE"
                     /locus_tag="SI621_002202"
     CDS             10683..11234
                     /gene="btuE"
                     /locus_tag="SI621_002202"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HEI4064921.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYLDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11234..11983
                     /gene="btuD"
                     /locus_tag="SI621_002203"
     CDS             11234..11983
                     /gene="btuD"
                     /locus_tag="SI621_002203"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HEI4064922.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12061..12525
                     /locus_tag="SI621_002204"
     CDS             12061..12525
                     /locus_tag="SI621_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4064923.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12772..13485
                     /gene="ydiV"
                     /locus_tag="SI621_002205"
     CDS             12772..13485
                     /gene="ydiV"
                     /locus_tag="SI621_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HEI4064924.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLFSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13548..14984
                     /locus_tag="SI621_002206"
     CDS             13548..14984
                     /locus_tag="SI621_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEI4064925.1"
                     /translation="MTLSFITRWRDELPATYTTLSPTPLNNARLIWHNAELANTLGIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVATSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQYSAASPLRDEFIDRAAFDDW
                     FAHYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(14988..15179)
                     /gene="hemP"
                     /locus_tag="SI621_002207"
     CDS             complement(14988..15179)
                     /gene="hemP"
                     /locus_tag="SI621_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEI4064926.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15310..16356)
                     /gene="aroH"
                     /locus_tag="SI621_002208"
     CDS             complement(15310..16356)
                     /gene="aroH"
                     /locus_tag="SI621_002208"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HEI4064927.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDSRF"
     gene            complement(16513..17346)
                     /gene="ppsR"
                     /locus_tag="SI621_002209"
     CDS             complement(16513..17346)
                     /gene="ppsR"
                     /locus_tag="SI621_002209"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HEI4064928.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17679..20057
                     /gene="ppsA"
                     /locus_tag="SI621_002210"
     CDS             17679..20057
                     /gene="ppsA"
                     /locus_tag="SI621_002210"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEI4064929.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRSDMGLTNVEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20114..21760)
                     /gene="fadK"
                     /locus_tag="SI621_002211"
     CDS             complement(20114..21760)
                     /gene="fadK"
                     /locus_tag="SI621_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HEI4064930.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQLVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCCMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21817..22110)
                     /locus_tag="SI621_002212"
     CDS             complement(21817..22110)
                     /locus_tag="SI621_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEI4064931.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22107..23396)
                     /locus_tag="SI621_002213"
     CDS             complement(22107..23396)
                     /locus_tag="SI621_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEI4064932.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDVLEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRIVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23452..24390)
                     /locus_tag="SI621_002214"
     CDS             complement(23452..24390)
                     /locus_tag="SI621_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEI4064933.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTAH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24410..25174)
                     /locus_tag="SI621_002215"
     CDS             complement(24410..25174)
                     /locus_tag="SI621_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HEI4064934.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQIATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGCGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPINQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEYLKKALN"
     gene            25490..>26069
                     /locus_tag="SI621_002216"
     CDS             25490..>26069
                     /locus_tag="SI621_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEI4064935.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNY"
BASE COUNT         6513 a         6225 c         6764 g         6567 t
ORIGIN      
        1 ttatagcagc ataatatgtt tctacagttt tataatgatt atgatgaatg gctatatgaa
       61 cagctgacat attgtcatga tttttagcgg ccaacaatgt gaataactga cattgactaa
      121 aagaatattt atttgcaaaa gtacccagcg ttgatatata agataacacg acgtctttat
      181 tacccttgct catggcgatg tataaagcag gggttccata tgctgttgcg ccttttaata
      241 aatccatgat gttaattttg ctgaggttat atttaacagc gattccataa actttagaga
      301 ggaaccgtgt gacacacaaa gggtgattat tttccattgc ggcatataat cctggctcac
      361 catcacgtgt tttcattgct aaaatatcca tcaccatttt tttgcttaaa agctcttgtg
      421 ttattggagt ggttaatgca ttcagaaaga tatcgattat ttcatcgaat ccatacaaca
      481 tggagatata gagtccaggt gtttcgttag cacttttagt ttgcagtaat ttaaccagat
      541 tatcttcatg aatgatatga ttatctacca gtgactgaat ttcttgcata aatattttta
      601 cgttgtcagt atgtccgttc atcatagcca gaaataaagc aggtgtacca acgctattct
      661 gcgcagttat tatttcaatg atttcttttt cagggagtat gggtaactgt ttgaataatg
      721 caataagctg ttgagtctgg cctgtggcca ttattaacaa cattgccgat ggatgtatag
      781 gggcaaatgg cattcgtgat gcaatacccg cgaaaatagc ccctggaact ccctcaggta
      841 ataggggcag aaaagcaatt gcacagttgc gaacatacat atcagcccag gtctgagcat
      901 cgggacctga actgatgaat ttatcgagcg agagaaagcc ccgtgctgtc ctgtgggtgc
      961 ctctgacggc aacgttggta tcgttcgggt cgtaaacgga cacaacataa tttaattgcc
     1021 catacgctga ctgctttatt cgtagcctta ctgacaaagt atgattgtct atatataaaa
     1081 tcgcggacat atgactgaca ttgtttgcca tcattcgaca gaagaaaata tctaaagcga
     1141 agccaaaatc cttactcgcg ataacgcgac tccctgcagg aggatggcga agtaatgatc
     1201 tgaactccgc gtcccggact aagggaacat gctgctgtat ggattcaacg gaggagtaat
     1261 gtttaatcag attgactttg ccttttccat ttctcagtga atcaagtgcg tattgcaatg
     1321 acagatggcg acaaaaaata gcgtcagaag gagattgcga aaaaaatgca ctaccattga
     1381 gatttactgt tggttgtata gttttttctc tatggatttt tttagacagt ttcatacaat
     1441 ggttacgatt ttcggcagtt aactgttcct gttcaatata atccagtatg cttcgcaaat
     1501 ggttagcttt aataggcgtg gtttccagta ataaggaaat gatttctttt ttatagtgta
     1561 atgtaatgag tatattttct gcatggatgg gtgaagtggc aaagttaatg gtaaaaaggg
     1621 cagtggaata acaattttgt gatatttttc gacagtttgc ggtaaattcc tgagtaacac
     1681 agtatgacag catcgaactt aagtcagagg tatcaagtag ggtgatgagt tggcgcgcaa
     1741 attgattaat aggctctgat gaaggcgttg gtatgtcgct atcgatgtgc attctatgag
     1801 gagacttata atgagttctg ataataatgt cgttttcact cataatctat tcgccttata
     1861 tatgtctgga ggttttgagg atgatgatta attattatcg ccagaacgaa atgtgcacga
     1921 aaaattgcta taagaattgt gtcattttta tcattgtgat atgaccactt cgtcattaat
     1981 cttatgatcg tttaaatagt acctccaaat cacgcaacga actttgctgg attcagttta
     2041 tgctgctgca aatccgctcg cagtaaacct ttcagacgct ccgtgatgtt atcagtggtt
     2101 ctttaagcgt tttgctggtg tactcactac aaacgaattg cgaatcgaat caatgtgaaa
     2161 cggaaaggta caatctccct ttttttagtg ttaacatcgc tcaaccgggt ggaaggtgag
     2221 aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga
     2281 tggtttcgca atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg
     2341 tcaccactgc aaataaggat ataaaatgcc tgttataact cttcctgatg gcagccaacg
     2401 ccattacgat cacgctgtaa gccccatgga tgttgcgctg gacattggtc caggtctggc
     2461 gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt gatgcttgcg atctgattga
     2521 aaacgacgca caactgtcga tcattaccgc gaaagacgaa gaaggtctgg agatcattcg
     2581 tcactcctgt gcgcacctgt tagggcacgc gattaaacaa ctttggccgc ataccaaaat
     2641 ggcaatcggc ccggttattg acaacggttt ttattacgac gttgatcttg accgcacgtt
     2701 aacccaggaa gatgtcgaag cactcgagaa gcggatgcat gagcttgctg agaaaaacta
     2761 cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt gaaactttcg ccaaccgtgg
     2821 ggagagctac aaagtctcca ttcttgacga aaacatcgcc catgatgaca agccaggtct
     2881 gtacttccat gaagaatatg tcgatatgtg ccgcggtccg cacgtaccga acatgcgttt
     2941 ctgccatcat ttcaaactaa tgaaaacggc aggggcttac tggcgtggcg acagcaacaa
     3001 caaaatgttg caacgtattt acggtacggc gtgggcagac aaaaaagcgc ttaacgctta
     3061 cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt aaaatcggta aacagctcga
     3121 cctgtaccat atgcaggaag aagcaccggg tatggtattc tggcacaacg atggctggac
     3181 catcttccgt gaactggaag tgtttgttcg ttctaaactg aaagagtacc agtatcagga
     3241 agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa aaaaccggtc actgggacaa
     3301 ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt gaatactgca ttaagccgat
     3361 gaactgcccg ggtcacgtac aaattttcaa ccaggggctg aagtcgtatc gcgatttgcc
     3421 gctgcgtatg gccgagtttg gtagctgcca ccgtaacgag ccgtcaggtt cgctgcatgg
     3481 cctgatgcgt gtacgtggtt ttacccagga tgacgcgcat atcttctgta ctgaagaaca
     3541 aattcgcgat gaagttaacg gatgtatccg tttagtctat gatatgtaca gcacttttgg
     3601 cttcgagaag atcgtcgtca aactctccac tcgtcctgaa aaacgtattg gcagcgacga
     3661 aatgtgggat cgtgctgagg cggacctggc ggttgcgctg gaagaaaaca acatcccgtt
     3721 tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa attgaattta ccctgtatga
     3781 ctgcctcgat cgtgcatggc agtgcggtac agtacagctg gacttctcct tgccgtctcg
     3841 tctgagcgcc tcctatgtgg gcgaagacaa cgagcgtaag gtaccggtaa tgattcaccg
     3901 cgcaattctg gggtcgatgg aacgtttcat cggtatcctg accgaagaat tcgctggttt
     3961 cttcccgacc tggcttgcgc cggttcaggt tgttatcatg aatattaccg attcacagtc
     4021 tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg ggcattcgtg ttaaagcaga
     4081 cttgagaaat gagaagattg gctttaaaat ccgcgagcac actttgcgtc gcgtcccgta
     4141 tatgctggtc tgtggtgata aagaggtgga atcaggcaaa gttgccgttc gcacccgccg
     4201 tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc gagaagctgc aacaagagat
     4261 tcgcagccgc agtcttaaac aattggagga ataaggtatt aaaggcggaa aacgagttca
     4321 aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc caggaagttc gcttaacagg
     4381 tctggaaggc gagcagcttg gtattgtgag tctgagagaa gctctggaga aagcagaaga
     4441 agccggagta gacttagtcg agatcagccc taacgccgag ccgccggttt gtcgtataat
     4501 ggattacggc aaattcctct atgaaaagag caagtcttct aaggaacaga agaaaaagca
     4561 aaaagttatc caggttaagg aaattaaatt ccgtcctggt acagatgaag gcgactatca
     4621 ggtaaaactc cgcagcctga ttcgctttct cgaagagggt gataaagcca aaatcacgct
     4681 gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt atggaagtgc ttaatcgcgt
     4741 gaaagacgat ttgcaagaac tggcagtggt cgaatccttc ccaacgaaga tcgaaggccg
     4801 ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa ggccttcaag tagcactgtc
     4861 tgtggagcct ttgggcttca caggcattgt tcgcctttgt ttcgtttatt aacaatgcga
     4921 agtggaagtt attaaaatgc caaaaattaa gaccgtacgc ggtgctgcta agcgcttcaa
     4981 aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac ctgcgtcaca ttctgaccaa
     5041 aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc atggtttcca aaggcgatct
     5101 gggcctggta atcgcgtgcc tgccgtacgc ataagccgtt aacgtttttt aactttttaa
     5161 ttagaatata gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc
     5221 gcacgtcaca agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta
     5281 taccgcgttg ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt
     5341 caacgtaagc gtcagttccg tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag
     5401 aacggtattt cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac
     5461 cgtaagatcc tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa
     5521 aaagcgaaag cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg
     5581 tttcaacgcc atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg
     5641 aaaagtacca aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact
     5701 ttagcacctg actccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa
     5761 gcctcccagt ggaggctttt tttgtatgcg cgttttgaaa aaattaagtc caacgaacca
     5821 gtgtcaccac tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg
     5881 cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agacaatgtg cgcgtcgaat
     5941 atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag
     6001 aagaacgccc ggcagccggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc
     6061 tgaatgcacg taaagcggaa ctggaaagcg cagcgctgaa tgcgcgtctg gcggcggaaa
     6121 cgattgatgt ctccctgccg gggcgtcgta ttgaaaacgg cggtctgcat ccggtgaccc
     6181 gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc
     6241 cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg
     6301 cgcgcgctga ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct
     6361 ctggcgtaca gatccgcacc atgaaagctc agcagccacc gattcgtatc atcgcgcctg
     6421 gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag
     6481 gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc
     6541 tgcgtaactt ctttgaggaa gatttgcaga tccgcttccg tccttcctac ttcccgttta
     6601 ccgaaccttc tgcagaagtg gacgtcatgg gtaaaaacgg taattggctg gaagtactgg
     6661 gctgcgggat ggtgcatccg aacgtgctgc gtaatgttgg catcgacccg gaagtttact
     6721 ctggtttcgc cttcggaatg gggatggagc gtctgaccat gttgcgttac ggcgtcaccg
     6781 acctgcgttc attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga
     6841 atagattatg aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag
     6901 cgatgcgctg gcaaatcaaa tcactatggc gggcctggaa gttgacggtg tagaaccggt
     6961 tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa
     7021 cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt
     7081 ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg gtagcgacca ttggtgctgt
     7141 tctgccgggc gatttcaaaa ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat
     7201 gctgtgctcc ttttctgagc tgggaatttc tgacgatcac agcggcatta tcgagctgcc
     7261 tgcagatgcg ccgattggca cagacatccg cgaatacctg aaactcgatg acaacaccat
     7321 cgaaatcagc gtaacgccaa accgtgccga ttgcttaggc attatcggcg tggcgcgtga
     7381 tgttgccgtg ctgaaccagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac
     7441 catcgacgac acgctgccga ttacagtcga agcgccggaa gcctgcccgc gctatcttgg
     7501 ccgtgtggta aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact
     7561 gcgtcgttgc gggatccgat ctatcgatgc agtcgttgac gtcaccaact atgtactgct
     7621 tgaactgggc cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt
     7681 gcggatggcg aaagagggcg aaacgctggt gctgcttgat ggcactgaag cgaagctgaa
     7741 tgctgacacc cttgtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg
     7801 tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc ctgctggaat gcgctttctt
     7861 cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc ctgcataccg atgcgtctca
     7921 ccgttatgag cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg
     7981 tctgctgatc gacatctgcg gcggtgaggc tggcccggta attgatatca ccaacgaagc
     8041 aacgctgccg aagcgagcca ccattacttt acgtcgcagc aaactggatc gcctgatcgg
     8101 ccatcatatt gccgatgagc aggtaactga tattctgcgt cgtctcggct gcgaagtgac
     8161 cgaaggcaaa gacgaatggc aggcagttgc gccgagctgg cgtttcgaca tggagattga
     8221 agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc ctgatgagcc
     8281 ggtgcaggca agcctgatta tgggtactca ccgcgaagca gatctgtcgc tcaagcgcgt
     8341 gaaaacgctg ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc
     8401 gaaagtgcag cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatttc
     8461 cgttgaaatg tcagcaatgc gtctttccct gtggaccggc ctgctggcaa ccgtggtgta
     8521 caaccagaac cgtcagcaga accgtgtgcg cattttcgaa agtggtctgc gtttcgtacc
     8581 agatactcag gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg
     8641 taaccgttac gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa
     8701 aggcgatctg gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc
     8761 agaagcgaat ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg
     8821 tattggtttt gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg
     8881 cactctggtg ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg
     8941 cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt
     9001 tcccgcagcg gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt
     9061 aaacttattt gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat
     9121 aagcctgatc ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt
     9181 cgccaaatgt gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta
     9241 tggcgcttac aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc
     9301 gggatgccaa agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg
     9361 gcgaacaggt gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc
     9421 cgggacgtaa cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct
     9481 tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat
     9541 ctgatctaac taaaaaggcc gctttgcggc cttttttctt ttcactgtcg aagagtcacc
     9601 gtaaaatcaa cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa
     9661 caacagcgac aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt
     9721 ctcttaagcc tttgcgccgg tgaacaatgg atttcgcccg gtgactggtc tactcctcgt
     9781 ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt
     9841 gcggcgctgg ctatatccgg cgcagtaatg caggcgttgt ttgaaaatcc tctggcagaa
     9901 cctggactac ttggcgtctc taacggcgca ggcgtagggc ttatcgccgc ggtgttgctt
     9961 gggcaagggc aactcccaaa ctgggcacta gggctgtgtg cgattgctgg cgcgcttatc
    10021 atcactttaa tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg
    10081 gctggcgttg cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc
    10141 acctctgttg atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac
    10201 tggcggcaaa gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag
    10261 tccaggccga tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc
    10321 ctgtggttct ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgtg
    10381 gcgctggcgg gtgctatcgg ctttattggt ctggttatcc cacatattct ccggctgtgt
    10441 ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg
    10501 ctgctggccg atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg
    10561 gtcaccgcaa cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag
    10621 ccgcaaaaag acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa
    10681 cgatgcaaga ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc
    10741 tggagaagta cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa
    10801 cgccgcaata tgagcagttg gagaatattc agaaagcctg ggccgatcgt ggttttgtgg
    10861 tgctgggatt cccgtgcaac cagtttctgg agcaagaacc gggcagcgat gaagagatta
    10921 aaacttactg caccactaca tggggggtga cgttcccgat gttcagtaag attgaagtta
    10981 atggcgaagg acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg
    11041 cgccggaaga gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc
    11101 ttgatgatat tttatggaat tttgagaaat ttttagttgg cagggacgga ttggtcatcc
    11161 agcgtttttc cccggatatg acgccggaag atcccattgt gatggaaagc attaaactgg
    11221 cgttggcgaa ataatgtcta ttgtgatgca gttacaggat gttgcggaat ctacccgcct
    11281 ggggccgctt tctggcgagg ttcgggctgg ggagatcctg catctggtgg ggccgaacgg
    11341 cgcgggtaag agtaccttac tggcgcgaat ggccggaatg accagcggta agggaagcat
    11401 tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc
    11461 ctatctttca caacaacaga cgccaccgtt tgcaatgccg gtctggcact acctgacact
    11521 gcatcagcac gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct
    11581 tgatgacaaa ctcggacgta gcaccaatca actttccggc ggcgaatggc aacgcgtacg
    11641 tcttgctgcg gtggtgctgc aaatcacacc acaagccaat cccgcaggtc aattgctgct
    11701 tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct
    11761 gagcgcgctg tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccatac
    11821 attgcgtcat gcgcatcggg catggttgct aaaaggtgga aaaatgctgg ccagtggacg
    11881 cagggaagag gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg
    11941 tctggatatc gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga
    12001 aaatcgggta attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga
    12061 atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa
    12121 gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac
    12181 cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg
    12241 gactgttcgg gatttgtggt tgtgacgatg cgcgatcgtt tcgatttgca gctgccccga
    12301 gaaaccaaag aacaagcctc tatcggcacg caaattgata aagacgagtt gctgcctggt
    12361 gacctggtct ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat
    12421 accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcactcgat
    12481 aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaaatgg
    12541 acggcgatga tacatctccg tccatttact cctgaataca gtagtatttt acagacaaaa
    12601 attccgtaaa tggattattc gtttataatc aggataatct aataaaagga cccctgaaaa
    12661 agtggttaac atggaacaaa tgaaagttac tccttctgta attgttatgt cttatggcct
    12721 catccctttt agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt
    12781 tttttggaga atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa
    12841 gaccttgtcg gcgttgagtt aattacccat ttctccagtg aagatggtac ggttcgtatc
    12901 ccgacgagtc gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag
    12961 cagttggaat tactaaaatc atgccaacac ttttttattc aacataaact ctttgcttgg
    13021 cttaatttaa cgccacaagt ggctacttta ttattagaca gggataatta cgcaggcgaa
    13081 ctattaaaat atccgtttat tgaactgttg attaatgaga attacccgca tctcgatgaa
    13141 ggaaaagata atcgcgatct tttttcctta tcgcaaatgt atccccttgt gttaggtaat
    13201 ttgggcgcag gcaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg
    13261 ctggataaaa gttttattca gcaacaaatc acgcatcgct ccttcgaacc gtttattcgt
    13321 gcgatccagg cgcaaatttc cccatgttgc aattgcatca tcgcgggggg cattgatacg
    13381 ccagaaataa tggcacagat tacccccttt gatttccatg ctctccaggg atgcctgtgg
    13441 cctgccgttc ccataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg
    13501 gtgtttaaga cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt
    13561 ttattacccg ttggcgcgat gaattgccag caacctatac aacactttcc cctacgcctt
    13621 taaataatgc ccggctgatt tggcataatg ccgaactggc taacacgctg ggtattccat
    13681 cgtcactgtt taaaaatggc gcaggtgtct ggggtggcga aaccctactg cctggtatgt
    13741 caccactggc gcaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg
    13801 atgggcgcgg cattttactc ggcgaacaac ggcttgctga tggcactaca atggactggc
    13861 atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac
    13921 gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga
    13981 cccgcgcttt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagccag
    14041 gcgcgatgct gatgcgtgtg gcaacaagtc atctgcgctt tggtcatttc gaacattttt
    14101 actatcgccg cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact
    14161 ggtcacatct tgcagatgat gaggacaaat accgtctctg gtttaccgat gttgtcgcac
    14221 gtaccgcatc gttaattgcc caatggcaga cggtcggctt tgctcatggg gtgatgaata
    14281 ccgacaacat gtcgctgttg gggctgacgc ttgattacgg gccgtttggt tttcttgatg
    14341 attacgaacc cggttttatt tgtaatcact cggatcacca agggcgttac agctttgata
    14401 atcaacctgc cgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg
    14461 tcgccgttga tgccctgaat gaggccctgg atagctatca gcaggttttg ttgacgcatt
    14521 atggacaacg gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc
    14581 tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc
    14641 gcatgctgag tctgaccgag cagtacagcg cggcgtcacc gctacgtgat gagtttatcg
    14701 atcgtgcggc atttgatgac tggtttgccc attatcgggt acgtttgcaa caagacgagg
    14761 ttaccgatag tgagcgtcag cagctgatgc aaagcgttaa cccagctctg gtgttgcgca
    14821 actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc
    14881 gcctgcatga ggcgttgcga aatcctttca gcgacagaga tgatgactac gtcagtcgtc
    14941 cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac
    15001 agcagcttgc cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc
    15061 agtttgccat ccggacctaa cagtgtctgg ctggtaattc gccggatggg ctgcggggaa
    15121 gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata
    15181 aaaattaata ataattgata taacaatgat tcttattatc aatattatgg cggccagagc
    15241 aagcagtaat ttgttgagaa gacaacaagt tgcgcagaaa tgcccattcc gtcaggaatg
    15301 ggcacgcatt cagaagcggc tatctaccgc agaggcgagt ttttcgacca ggcgttcggt
    15361 atcttcccag cccagacacg ggtcggtaat cgattgaccg taagtgagcg gctgaccgcc
    15421 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt
    15481 agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg
    15541 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
    15601 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
    15661 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccgttttt
    15721 gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcta cggcaatccg
    15781 cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc
    15841 acggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc
    15901 aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gtagggacgc ccagctcatt
    15961 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat agctgccgtt
    16021 taaatctgga tcggagatta gccctttcca gccgacaaca gttcgtggtt tttcaaaata
    16081 ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt
    16141 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
    16201 tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg tgacatgggt
    16261 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc
    16321 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
    16381 tctacggcag gactaccggg aagtgatgcg catcacgata aaccaatcgc agctgatttc
    16441 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aataagctaa
    16501 tgcactagtt ctctagtaca ttcggcggct aaggcccatg atatcgagga tcttggtggc
    16561 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
    16621 ggcttctact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
    16681 ccgacgttct tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
    16741 atgctgaagc ggtttgagcg acgcgggtag caccagatta tccatgtcgt cggcaataaa
    16801 ggggtagttt gccgcgcgga taccaaattg cattgccaga tacagactgg tgggggtttt
    16861 accacagcga gaaacaccga gcaggatcac ctgagcctga tccagattgc gcaacgaaat
    16921 gccgtcatcg tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag
    16981 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
    17041 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagcctt cactttgcaa
    17101 gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacgcctgt
    17161 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
    17221 aaacggcagc gtaatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
    17281 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
    17341 atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc
    17401 acagaagcgt agaacgttat gtctggttta taaaatgaac cttcaatttt attttttatg
    17461 aaaacagcat ttcattttta cggtttcgtt tataccgatg gtttatgtgg aaattgtcga
    17521 agagagcaga tttgcgcaac gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt
    17581 gaacgattca ccgttttttt catccggtta aatatgcaaa gataaatgcg cagaaatgtg
    17641 tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat ggctcgtcac
    17701 cgctggtgct ttggtataac caactcggca tgaatgatgt agacagggtt gggggcaaaa
    17761 atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc gttccgaatg
    17821 gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc
    17881 gcatttatga actgctggat aaaacggata ttgacgatgt tacccagctt gcgaaagcgg
    17941 gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg gaaaacgcca
    18001 tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa cgcctcgttt gcggtgcgtt
    18061 cctccgctac tgcagaagat atgccggacg cttcttttgc cggtcagcag gaaactttcc
    18121 tcaacgttca gggttttgac gccgttctcg tggcagtgaa acatgtattt gcttctctgt
    18181 ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc
    18241 tctccgccgg tgttcagcgg atggtgcgct ccgacctcgc atcttctggc gtgatgttct
    18301 ccattgatac cgaatccggc tttgaccagg tggtgtttat cacttccgca tggggccttg
    18361 gtgaaatggt cgtgcagggt gcggttaacc cggatgagtt ttacgtgcac aaaccgacac
    18421 tggcggcgaa tcgcccggct atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg
    18481 tctacgcgcc gacccaggag cacggcaagc aggttaaaat cgaagacgta ccgcaggaac
    18541 agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa caggccgtac
    18601 aaattgagaa acactacggt cgcccaatgg atattgagtg ggcgaaagat ggccacaccg
    18661 gcaaactgtt cattgtgcag gcgcgcccgg aaaccgtgcg ctcacgcggt caggtcatgg
    18721 agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct atcggtcatc
    18781 gcatcggcgc gggtccggtg aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac
    18841 ctggcgacgt gctggttact gacatgactg acccggactg ggaaccgatc atgaagaagg
    18901 catctgccat cgtcaccaat cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg
    18961 aactcggcat tccggcggta gtgggctgtg gtgatgcaac agaacggatg aaagacggtg
    19021 agaacgtcac tgtttcttgt gccgaaggtg ataccggtta tgtctatgcg gagttgctgg
    19081 aatttagcgt gaaaagctcc agcgtagaaa cgatgcctga tctgccgttg aaggtgatga
    19141 tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac gaaggcgtgg
    19201 gccttgcgcg tctggaattt atcatcaacc gtatgattgg cgtccaccca cgcgcactgc
    19261 ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt
    19321 ttgattctcc gcgtgaattt tacgttgggc gtctgactga agggatcgcg acgctgggtg
    19381 ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg aacgaatatg
    19441 ccaacctggt cggtggtgag cgttacgagc cagatgaaga gaacccgatg ctcggcttcc
    19501 gtggcgcggg ccgctatgtt tccgacagct tccgcgactg ctttgcgctg gagtgtgaag
    19561 cagtgaaacg tgtgcgcagc gacatgggac tgaccaacgt tgagatcatg atcccgttcg
    19621 tgcgtaccgt agatcaggcg aaagcggtgg ttgacgaatt ggcgcgtcag gggctgaaac
    19681 gtggtgagaa cgggctgaaa atcatcatga tgtgtgaaat tccgtccaac gccttgctgg
    19741 cagagcagtt cctcgaatat ttcgacggct tctcaattgg ctcaaacgac atgacacagc
    19801 tggcgctcgg tctggaccgt gactccggag tggtgtctga actgttcgat gagcgcaacg
    19861 atgcggtgaa agcactgctg tcgatggcga ttcgtgccgc gaagaaacag ggcaaatatg
    19921 tcgggatttg cggtcagggc ccgtccgacc acgaagactt tgccgcatgg ttgatggaag
    19981 aagggatcga tagcctgtct ctgaacccgg ataccgtggt gcaaacctgg ttaagcctgg
    20041 ctgaactgaa gaaataaaat aaatccccgg cggcgttcag tcgccgggtt tatgtgatcc
    20101 ccgaagatga aacttattca atctcttcac agacatcctg agttaatctc cgcataatat
    20161 ctttccttaa caaaaacttt tgtattttac ctgaggcagt gcgcggtagt ttttcgatta
    20221 ccacgatatg ttcaggatat ttatatttcg cgacccgttt acggctaaaa aaagccacca
    20281 cctcttccag cgataatgaa tgatgcggtg ctttcagcac gacataagcg catgagcgtt
    20341 cacctaaacg ttcatcgggc atcgcaacca cacaggcatc gtgaatttta ggatgctgca
    20401 ataaaatatc ttctacttca cggctgctaa tattttcgcc gccgcggaca ataatatctt
    20461 tcttgcgccc ggttattttt atatagccgg cctcatccat gcagcagaga tcgccgctgt
    20521 aataccagcc ttcttcatcc agggcacggg cggttaattc aggttcatca aaatacccca
    20581 taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt aaggtcttgc
    20641 gtgcgtcatc gaccacttta atctcaacac ctgcggcagc gtaaccatcg gtgtgcataa
    20701 agcgcgacaa aggatcgtcg agattcacca ctgcgtgcgg cgaactttct gtggaaccat
    20761 aaacacttaa taatttaatg ccgcgctgct ggcattcacg cgccactttt ttggggattg
    20821 tggtcccgcc gcaaagaaag aagcgcagcg ctgaaaggtc agcgggttgt ttctccagta
    20881 aattcaaaag atcatagaca aacggcgttg cgccgagcat acaggtgcaa cgctgctgct
    20941 caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg cgagcgccaa
    21001 ttaagaatgg tgccgttacg ccatgcagaa agcccgttgc atgaccaagt ggcgcaggca
    21061 tcataaagac atcctgccag gtcagattca accgcgcgca ataagcccgc tcactggcga
    21121 gaatattgtt atgcgttagc atcacgccct ttggcagacc ctcggttccg gaggtaaaca
    21181 gcaccgcggc taattcatcg ccgtggaccg ttatcgccgt ggttagagga gtattgtcgg
    21241 cgataatctg acttaatgag agagcagagg tggcgggagc cagtttgtcc acgccgacaa
    21301 gttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc ggacgcgttt
    21361 gtttaaacaa cgtcggagca aagaacattt ttgcctgaca cttattgagc acccatacta
    21421 gttctgcttc ccgccaggaa ggcaacagcg gtacggaaac cgcaccgatt ttcaggcagg
    21481 caagatagat aacggtaaat tcacaccagc caggcagttg aaatgcgatg cgatcgcctg
    21541 attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga tcgagcgcgc
    21601 tataggtgta cgatgctcca tgattatcga ccacggcaat tttgtctggc atcgcacgag
    21661 cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc tgacgatacg
    21721 ccgcacgacg ttgttcgtta aacgttaatg tcactttcat tttggactct cgagacagaa
    21781 catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa aatcaatacc
    21841 gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttgccgcaca gcacccgaca
    21901 ggtgccgcac tccagacaac cggcagaatc aaaatgaatg ttgccggcgt catcctgctt
    21961 ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat cgggatttgc
    22021 cgccaaaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc ctaatttgat
    22081 gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat gccatctttc
    22141 agcaagttga tcagcccaat ttttttcgcg tgtcccatga tcattttgcg taccggctgg
    22201 tttggtttgc cgtcaatggt gaacatatcg ttcatgatgt cggcgacaat gcgcggatac
    22261 tgactaaaca ggcgcggatt ttccatcagc gccgggatct tgcggaaatg ctgcatatcg
    22321 cgcataacac aactttgttc cagctcacgt ttgtattgcg ccagactgct ggcggagaaa
    22381 tccgcgcgtt ctttggcggc aatcactgtt gtggcggcag cctgagctga tgcaatggct
    22441 aaatccatgc cgcgaactgt aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg
    22501 atcatcacgc catcgttaac cagctgcggc accattgcca gaccgccttc cggcaccata
    22561 tgcgcggaat attcaagcag tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt
    22621 ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc acccaatcca
    22681 caaaccagcc ccaacgatat ggaatcctta ttggtataga ggaatccgcc acccatcagg
    22741 ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt aatattaaag
    22801 cgatcgttga tctgttctgg tgtgaggcca ataacctctt taacaccaac agcgtaatga
    22861 tgcggatcgg aagcggaaac cattcccagc gagcggccaa gcatcgagtt aacgccatca
    22921 gccagaatca ccacattcgc ttcaagaaca tcatccccgg cctgcacgcc agtgaccttg
    22981 tttccttcac gaaccagcgc atcgacgcgt acgcccggga taaactgcgc gcccgcctgc
    23041 tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata agacgcgtgt
    23101 tgcggaacat ctggttgctc gcggtgaaag tcgagggtaa cggcgctttc ttcggttaag
    23161 aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatgctgc aaagcctgga
    23221 ataatcgctt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt acatccggca
    23281 ctgtcgccgc gttctatcac caggacatcc agcccggctc gcgccatgac cagtgcggca
    23341 acgctaccgg caacgcccgc accgaccaca atggcatcaa atttgtcatc cgacatgcgt
    23401 tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggga attagcggct
    23461 caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac cgtagtcggc
    23521 atagttgaag attggcgcat ttttatcttt attgatggcg acaatcactt ttgcgccgtt
    23581 gccgccaacc atatgctgga tctgcccgga gatccccagc gtcaggtaga gatcggattt
    23641 cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct cgccttcggc
    23701 aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt ggaccatttt
    23761 caggtcatcc tgcgctgcca gaccacgacc gacgccaacc acacgttttg ctttgctcag
    23821 gtccacgctg cttgccgctt tcgcacggcg ttcgcgacag agaatttcat gacgcggggc
    23881 aacataaggt accgtttctg tcgggcactg atgagcggta tcactggtgc acggttcctg
    23941 aacaccgggt gcaagggtaa tgatcgccag cgggctgttg atcttttcct gtgcgaacgc
    24001 taacccgcca tacatccggt gttcggcgca aatgtgacca tcgacaatat ccaccgcagt
    24061 ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgctgcca gagctttacc
    24121 gcgtttcgtc gcggccagca gcaacataga aggatgttta tctttcagca gggcagcaat
    24181 gctttcggcg taattttcag tacgttgcag cgcatcattt tgctcaagaa cataaataca
    24241 ttttggacca taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac
    24301 ctgttggccc cattgctgcg cgccgccaaa cagttcagca taacgttcag gattatcgct
    24361 aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt cagttcaggg
    24421 ctttcttcag atattccgcc agctcggcaa tggcctccgg cgaatcgtta tcgagaatga
    24481 tgtgcttacg ttctgtttgc ggcggtacgc gaatgccggt cagctcagca agtggcgcgc
    24541 tctggctcca gccaatgtca cttgcctgcc actgatttat cggtttttta cccgcgccga
    24601 gaatggcttt catcgaagga atgcgtggca tgttaatatc ggaggtgacg cagagcacgg
    24661 ctggaacaga gagctcaata acttcaacat catcttcaag cgtgcgttca atcaccagtg
    24721 tattgccctg acgctgaata gcactcactg cattaatcac cggaagttgc agaatttctc
    24781 cgaccagcaa gccaacctgc tgggcataaa ggtcaccgca accttcacca aagatcagta
    24841 aatcgaagcc gatcttttca actgctgccg ccagtgcttt tgcggtatcg agaggcagtg
    24901 catgttcaag ttgcgcatcc tgcaccaaat acaggctatg cggcccgcgg gatagcacgt
    24961 ctttgcgtac tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg gctatctcat
    25021 cgtcatctgt tgcaatctgg cttgcagctt caatggcatt gagatcgaac tggctgattt
    25081 tggcgtcggc attgtcgaaa ttcagggtat attctggagt gacaacaatg tcctgttctt
    25141 caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt cgcatacgga
    25201 accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt
    25261 ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa
    25321 acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt gcattcagtg
    25381 taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac acgccgtatt
    25441 ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca tgtatcaacg
    25501 ctgttttgat aacgccagcg aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg
    25561 ttttgcattt agcgatgatc caaaatggga gtccggacat cacgttcatg acaatgaaac
    25621 cgagttgatt tacgtcaaga aaggggtagc aaggtttacc atcgattctt cgttatatgt
    25681 cgcgcacgcc gatgacattg tggtgataga acgtggcagg ctgcatgcgg tggcctctga
    25741 cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt ttcagggggc
    25801 tgaggaaaat cagctactgc aaccgcattc ttgcccggta attgccgcag ggcagggaaa
    25861 agaagttatt aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag
    25921 ccaaacatct tcgttatggg acgcatttgc ctatacgtta gcaattcttt actatgaaaa
    25981 ctttaaaaat gcttatcgtt cggagcaggg atatattaaa aaagatgttc tgataaaaga
    26041 tattcttttc tatctgaata ataactatc
//
LOCUS       DARNVM010000060        25881 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.060, whole genome shotgun sequence.
ACCESSION   DARNVM010000060 DARNVM010000000
VERSION     DARNVM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.060"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..20403
                     /locus_tag="SI621_002217"
     CDS             <1..20403
                     /locus_tag="SI621_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEI4064936.1"
                     /translation="GQTTAFPWGWLAGAAVGGGALGALLASGGDDDSKTEVVNNPPPA
                     EPGNATPSFLVTDNQGDQRGILSANDTTDDTTPTFSGSGQAGATIQIKDSNGDTIAST
                     QVGSDGRWSVDLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASVQVATTAGDN
                     IINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQVPTADAQALG
                     EGNQAVLVSGKDATGNTVTGVQLLTVDTQPPTLAINTIAQDNIISAAEHNVALVLSGT
                     SNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNASVSDRAGNS
                     TSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGDTVTVKLGTH
                     VLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDTAGNTGAASRAITLVGVSPLI
                     TINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQADGSWSLTV
                     PAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTADNIINAAES
                     GQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGALADGNATVTA
                     SVNDIAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISGTVTGAQAGD
                     IVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLVDGSYPINVSVTDRAGNTGSQSLTV
                     TVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNGQNYTTTTDA
                     SGNWSVTVPASAVTALGQANYTVTAAVTSNIGNSNTASHNVLVDSALPGVTINPVATD
                     DIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNFTWSVSVPAADIQAL
                     GNGDLTVSASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIEHGQALVVTG
                     SSSGLAEGTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSTWPAGTVNIAVSGESSAG
                     NSVSITHPVTVDLTPAAIAINTIATDDVINAAEKGADLTLSGTTTNVEPGQTVTVTFG
                     GKNYTASVASDGSWTATLPAADLTALPEGSASAQASVSNINGNSASAVHNYSVDSSAP
                     TIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQATVQADGSW
                     SINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTINTIAGDDIIN
                     AAEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAADVTALGSGP
                     QTVMATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDIINATEKGADLQISGTSNQ
                     PAGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSA
                     SHNVQVNTALPGVTINPVASDDIINAAESGVAQTISGQVTGAAAGDTVTVTLGGKTYT
                     ATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGLRVD
                     TVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADGTWSLGVPAA
                     DVGNWPAGTVNITVSGTNTAGTTTTITHPVTVDLAAVAISINTVSGDDVINAAEKSAD
                     LTLSGSTSGVEAGQTVTVTFGGKTYTATVAGDGSWTTTVPAADLSALRDGDATVQASV
                     STINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSSNAEAGQTVT
                     VTLNGVTYTGTVQADGSWSVSVPTADLSNLTASPYTVSASVNDKAGNPATATHGLAVD
                     LTVPVLTINTVSGDDIINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTTLDASG
                     NWSVGVPAADVTALGSGPQTITATITDIAGNSDDASRTVTVNLTAPTIGINTIASDDV
                     INATEKSADLQITGTSNQPAGTTITVTLNGQNYTATTDSSGNWSATVPASAVSALGEA
                     SYTVTANVTDSAGNSNSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVT
                     GAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQAIGNGSLTVNASVTNVVGNTG
                     NGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVT
                     YGATVLADGTWSLGIPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVTVDLAGVAIT
                     INTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVESNGSWTVNVP
                     PADLAALPDGAGNVQASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAG
                     AGITISGTTTAQVGQTLTVTLNNNTYQTTVQADGTWSVNVPATDLSGLTASSYTVTAT
                     VSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDV
                     VTVTLNGKNYTTTLDASGNWTVGIPAADVTALATGSQTITASLSDRAGNSDSTTHNVT
                     VDLSGPTLTISTVSDDDIINSTEKTQDLTISGGSSGLATGTTVTVMLNGLAYSATTDS
                     SGNWSVTVPASAVGALGEAGYQISASATDSAGNNGSTTHTVNVESLLPGVIINTVAGD
                     DIINAAEIAVAQTISGQVTGTAVAGNTVIVTIGGNQYTATVQPDLSWSVSVPANVLQA
                     LGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVIT
                     GSGSGLAAGAALTVVINSVTYGATVLADGTWSVGVPAADVTNWPAGTVNIAVSGTNTA
                     GTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSDVEAGQTITVIF
                     GGKSYTTTVAAGGTWGLTIPAADLATLPDGAANVQASVSNVAGNSAQATHAYSVDATA
                     PSVTINTIASDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWSV
                     SVPTGDLANLTASPYTVSAAVSDKAGNPASATHNLTVDLAAPVVTINTVAGDDIINAT
                     EHAQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDVT
                     ITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINATEKGADLAISGSSNQPA
                     GTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDSDGNSGSASH
                     NVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGATAGDTVTVTLGGATYTAT
                     VQANLSWSVDVPAAALQALGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDTV
                     AGDDVVNIIEHGQALVITGSSSGLATDSNVTLTINGQTYVAAVLADGSWSVGVPAADV
                     SAWPAGTVTITASGSTTAGNPVSVTHPVTVDLTAVAVSINAITADDVINAAEKGAALT
                     LSGSTSGVEAGQTVTVTFGGKTYTASVAANGSWSTSVPAADMAALRNGDASAQASVSN
                     VNGNNATTTHAYSVDASVPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTVT
                     LNGANYTGTVQTDGSWSISVPPADLSALTASNYTVSAAVSDKAGNPASVNHNLTVDTS
                     VPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGNW
                     SVGVPASVVSALANGTVTINASVTDAAGNSGSATHQVTVNTGLPSITFNAISGDNVLN
                     ADEKGQPLTISGSSTGLATGAQVTVTLNGHNYSATTDAAGNWTLTVPVSDLAALGQAN
                     YIVSASATSAAGNTASSQANLLVDSGLPGVTINTVAGDDIINAAEAGAAQTISGVVTR
                     AAAGDTVTVTLGGNTYTAQVQADLSWSVSVPAADLQALGNGDLTITASVTNANGNTGS
                     GTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGVPLTITINGTAY
                     SATVQADGSWSVGIPAANVSAWPAGALTVEVDGQSSAGNPVGVSHPFTVDLTAVAISI
                     STVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVPA
                     ADLAILPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNATEAGS
                     PLIISGTSTAETGQTVTVTLNGATYSGNVQADGSWSVSVPPSALGALSASNYTVSATV
                     NDKAGNPGSASHNLAVDTTAPVLTINTVVGDDIINDAEHAQALVISGTSTGGEAGDVV
                     SVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNSDDASRTVTV
                     SLTAPVISINTIAGDDVINATEKGSDLALSGISDQPAGTAITVTLNGQNYSATTDSSG
                     NWSVTVPASAVSALGEASYSVTASVTNAQGNSSTASHNVQVNTALPGVTINPVTTDDI
                     INAAEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALGN
                     GELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHSQAQVITGSS
                     SGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGTQ
                     TSITHPLTVDLTTVAVSINSITSDDVINAAEKGAALTLSGSTSGVEAGQTVTITFGGK
                     TYTTTVAANGSWSTTVPAVDMATLRDGDASAQVRVTNVNGNSATATHEYSVDSAAPTV
                     TINTIASDNIINASEAAAGVTVSGTSTAETGQTLTVTLNGTNYQTTVQADGSWSLTLP
                     ASDLTALANNGYTLTASVSDLAGNPGSASKGVTVDTTAPVISFNTVAGDDVINNVEHT
                     QAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTISA
                     TITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSGTGTNFAAGT
                     VVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASRS
                     VAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATVA
                     ANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQTTHALTVDTVAPTVTIATVAG
                     DDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWTATVGSGGSWSVFIPAQQFAGL
                     SDGSYTISATVSDQAGNPGSASRGVTLNGDVPSVTINTFAGDDVVNAAEHGSSLVISG
                     TTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSVDVTALADGNAYVINASVSNAIG
                     NTGSSNHTITVDLSAPAMGINIDSLQADTGLSSSDFITSVSPVVVNGSLTAALASNET
                     AQISIDGGVTWTTLTVTGTTWRYNDSRTLTDGSYLYQVRVIDAAGNVGATDSQNVVID
                     TIAPDPAVKTIAINAITTDTGLITNDFVTSDTTLAVSGTLGAALSSGEFAQISIDGGT
                     TWQNLSVSGLTWTYLDGRTLSDGNYNYQVRVIDTAGNIGATASQIVTVDTTAPLASKT
                     IAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVTWTTLTVIGT
                     SWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSITITGISDDT
                     GASSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADGLNWSYVDGR
                     TLTNGTTTWQVRVVDLAGNVGATSSQSAQIDTVNPAQVLTIASISTDTGSSATDFITS
                     DTTLTLTGSLGAGLASGEVAQISLDGGATWITLTTNGTQWTYTDSRTLTDGSYVYQVR
                     VLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATDDTTPLLNG
                     VLSGPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARVVDLAGNIT
                     ASSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVINGKTYTSE
                     PGGAVVVDPAHNTWYVQLPDTDALAASATAYNVTAQVKSSAGNGNNANISNGTVTVNA
                     AIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTALTLYQSGN
                     NYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVGTLTWYGSI
                     VAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVGGAVTGYLS
                     LNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNTFLSGAGSG
                     AMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAVGATATTYAS
                     QFSVAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQSGTTSGVAAMDY
                     DWDGAVDVLVTKQSGSVYLIRNTNTVSYGTSLHLRITDPNGINVYYGNTVKLYNSAGV
                     LVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQTVNTSWGG
                     LQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWVYSSGTGTW
                     LANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFNDTLTGSSGD
                     NQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWEGTADTDRI
                     DIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQIDRDGTGGT
                     FAATNVVTLTGVHTDLATLLANHQLMVV"
     gene            20407..22569
                     /locus_tag="SI621_002218"
     CDS             20407..22569
                     /locus_tag="SI621_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEI4064937.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSTDSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVTDIEDNIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTTRRVVLQEKNKGSAA"
     gene            22566..23741
                     /locus_tag="SI621_002219"
     CDS             22566..23741
                     /locus_tag="SI621_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEI4064938.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            23738..24145
                     /gene="cueR"
                     /locus_tag="SI621_002220"
     CDS             23738..24145
                     /gene="cueR"
                     /locus_tag="SI621_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEI4064939.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24349..24771)
                     /locus_tag="SI621_002221"
     CDS             complement(24349..24771)
                     /locus_tag="SI621_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064940.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(24825..25841)
                     /locus_tag="SI621_002222"
     CDS             complement(24825..25841)
                     /locus_tag="SI621_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEI4064941.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLTNTQKRVADNSQQINTLNNHFDSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         5870 a         7621 c         7658 g         4732 t
ORIGIN      
        1 ggccagacga cggctttccc gtggggatgg ctggcggggg cggcggtagg tggcggcgcg
       61 cttggcgctt tactggcaag cggtggcgat gatgactcaa aaactgaagt ggttaacaac
      121 ccaccgccag ccgaacccgg caacgccacg ccatcgtttt tggtaaccga taatcagggc
      181 gatcaacgcg gtattctttc cgccaacgat accaccgatg acactacgcc aacctttagc
      241 ggcagcgggc aggcgggggc gaccatccag attaaagaca gcaatggtga caccatcgcc
      301 agtactcagg tcggtagcga tggacgctgg agcgtcgatt tgcccacgca aagcgcgggc
      361 gaacatacct ggtcagtggt gcaaatcgtc ggcagtacca tcactgacgc cggttcgata
      421 acgttaacca tcgacaatag tcaggccagc gtgcaggttg ccaccaccgc aggcgataac
      481 attattaacg ccagcgaaca ggccgccggg tttacgcttt ctggcaccag tagtcatctg
      541 gcacagggaa cagaactcac cgttacgcta aacggcaaaa cctacaccac cagcgtaggc
      601 gctaacggtg cctggagcgt gcaggtaccg accgccgatg cgcaggcgtt aggcgaaggg
      661 aatcaggcag tgctggtcag cgggaaagac gccacaggca ataccgtcac cggcgtgcaa
      721 ctgctaacgg tcgataccca accgccaacg cttgccatca acaccatcgc tcaggacaac
      781 atcatcagtg ctgcggaaca taacgtcgcg ctggtactga gcggcacgtc gaatgcagaa
      841 gcggggcaaa ccgtaacact gaccgtcaac gggaaaagcc atacagcaac cgtcggtagc
      901 gacggaacct ggcaagtgac gctgcctgcc acggaagtcc aggcgctggc ggagggtaat
      961 tacgctgtca atgccagtgt cagcgatcgg gcagggaaca gcaccagcca cagcgcgaat
     1021 ttcacggtag atacctcagc acccgtggtc agtgttaata ccgtagcggg cgacgatatt
     1081 cttaataatg ctgagcaggc cgtcgcgcag atcatctccg gacaagtcag cggtgcttct
     1141 cctggcgata cggtaacggt gaaattgggc actcatgtcc tgacgggcat tgtgctggca
     1201 gatggcagct ggaatgtggc gctggaccca gcggtaaccc gcacgctgga tcgcggagcc
     1261 aatacgattt tcgtcaccgt gacagatact gcaggaaata ccggcgcggc gtctcgagca
     1321 atcacgctgg tcggtgtttc tccgttaatc accattaaca ccgtctccgg cgatgacatt
     1381 atcagtggcg cagaaaaagg tgcgccactg acccttaccg gtagcactca acaggccgag
     1441 acaggacaaa ccgtcacagt aaccctggct ggacagagtt ttaccactac cgtgcaggct
     1501 gatggctcct ggagtctgac ggtacctgcc gccgcgatgg gaaatctgcc tgacggcgcg
     1561 gtggcgatta ccgcttctgt gacggatctc agcggcaata ccggcaacac ctcccgcacc
     1621 attaccgtcg acagccaggc cccggcgttg agcatcgatc cactgaccgc tgataacatc
     1681 attaacgccg ccgaaagcgg gcaggatttg cccatcaccg gcaccaccga cgctcagcca
     1741 gggcagacgg tgaccgttac gttgaatggg cagacgtacc agggcgtcgt gcagccagac
     1801 ggcacctgga gcgtgaccgt tcccgccgcc aacgttggcg cactggctga cggcaacgcc
     1861 accgtcaccg ccagcgtgaa cgatatcgcc ggtaacccga ccagcgtctc acgcgtggcg
     1921 ctggtcgatg ccacgccgcc ggtggtaacc attaatccgg tggcgaccga taacgtcatc
     1981 aacacgccgg aacacaccca ggcgcaaatc atcagcggca cggttactgg cgctcaggcg
     2041 ggcgatatcg tcaccgtgac gctgaataat gtggattaca caacggtggt ggatggttcc
     2101 ggcaactgga gcctgggcgt tccggcttcc gttgtcagcg gactggtgga tggcagttat
     2161 ccgatcaacg tgtcagtgac tgaccgcgcc ggaaacacgg gcagccagtc attgaccgtc
     2221 acggtcaata ctgccgcgcc ccttatcggc attaacagca ttgcgggcga tgatgtgatt
     2281 aacgccagcg aaaaaggggc cgatctccag atcaccggca ccagcgatca gcctgttaat
     2341 accgccatca ccgtgacgct gaacgggcaa aattacacca ccacgaccga cgcctccggc
     2401 aactggagcg tcaccgttcc ggcatcggcg gtaaccgcac tgggccaggc caactacacg
     2461 gtaacggcgg cggtgacgag taatatcggc aacagcaaca ccgcaagcca taacgtgctg
     2521 gtcgacagcg cgctgcccgg cgtgaccatt aacccggtgg caaccgacga tattattaac
     2581 gccgccgaag cgggcgttgc gcaaaccatt agcgggcagg ttactggcgc ggaagatggc
     2641 gacacggtaa ctattacgtt gggcggtaat acttatacgg cgacggtggg cagcaatttc
     2701 acctggagcg tgagcgttcc ggcggcagat attcaggcgc tgggtaatgg cgatttaacg
     2761 gtgagtgcct cagtcaccaa tcaaaacggc aacaccggca gcggcacgcg ggatatcacc
     2821 atcgacgcca atctgcccgg cctgcgggtc gatacggtgg cgggcgatga tgtggtcaat
     2881 atcatcgagc acgggcaggc gctggtggtc accggcagca gttcggggct ggcagaaggc
     2941 acgccgctta ccgtcacgat taataatgtg gaatacacca ccgcggtgca ggccgatggt
     3001 agctggagcg tgggcgtcac tgcggcgcag gttagcacct ggcctgcggg gacggttaat
     3061 attgctgtat ccggggaaag tagcgccgga aactcggtga gcattacgca tccggtgacg
     3121 gtcgatctca ctccggcggc gatcgccatc aacaccatcg ccacggatga cgtgattaac
     3181 gccgcagaaa aaggcgctga tttaaccctt tccggcacca ccactaacgt agaacccggt
     3241 caaaccgtca ccgtcacctt tggcgggaaa aattacaccg ccagcgtagc aagcgacggt
     3301 agctggactg ccaccttacc tgccgccgat ctgacagcat taccagaggg cagcgcctcc
     3361 gcgcaggcca gcgtcagcaa tattaatggc aatagcgcct cggcggtgca taactacagc
     3421 gtcgacagca gtgccccgac catcattatc aataccgtcg ccagcgacaa tatcgtcaac
     3481 gccagcgaag ccgatgcggg tgtgacggtg agcggcagca ccaccgctga agcggggcag
     3541 attgttacgg taacgctcaa tagcccgacc gtgcagacct atcaggcaac ggtgcaggcg
     3601 gacggcagct ggagcatcaa tattccggcg gcagatcttg aggcattgac cgatggcagc
     3661 cacaccctga ccgccacggt caatgacaaa gcgggcaacc cggcgagcac cacgcataat
     3721 ctggcggtgg atctcaccgt tccggtactt accatcaaca ccattgcggg cgacgatatt
     3781 attaacgccg ccgaacacgg gcaggcgctg gtgatttccg gttccagcac tggcggagaa
     3841 gcgggggatg tcgtcaccgt cacgctaaac agtaaaacct acaccacgac gctggatgcc
     3901 tccggcaact ggagcgtcgg cgttccggcg gcggatgtca cggcgctggg tagcggcccg
     3961 caaaccgtga tggcgaccgt taccgatgcg gcaggtaata gcgacagcga aacgcacacg
     4021 gtcaccgtta accttaccgc gccaaccatt ggcattaaca ccatcgctac cgatgacata
     4081 attaacgcca ccgaaaaagg cgcggatctg caaatttccg gcaccagcaa tcagccagcg
     4141 ggcaccacta ttacggtgac actgaacggg caaaattaca ccgccacgac cgatgcttcc
     4201 ggcaactgga gcaccacggt gccagcctcc gctgtcggtg cgctgggcga agccagttac
     4261 acggtgacgg cgaacgtcac cgatagcgcg ggcaacagca attccgccag ccataacgtg
     4321 caggtcaata ccgcgctgcc tggcgtcacc attaatccgg tggcgagcga cgatattatt
     4381 aatgccgccg aatcgggcgt ggcgcaaact atcagtgggc aggtgacggg cgcggcggcg
     4441 ggcgataccg tcaccgtgac acttggcggg aaaacttaca ccgctaccgt gcaggggaat
     4501 ttaagctgga gcgtggacgt tccggcggcg gatattcagg ccatcggtaa tggcgatctc
     4561 acggttaacg cctcagtgac caacggcgtg ggtaataccg gcagcggttc gcgcgatatt
     4621 actatcgacg ccaatttgcc aggtctgcgc gtcgataccg tggcgggcga tgatgtggtt
     4681 aacagcatcg agcacggtca ggcactggtg ataaccggta gcagcagcgg gctggcggcg
     4741 ggcgcggcgc tgacggtggt gatcaataac gtcacctatg gcgcgacagt attagccgat
     4801 ggcacatgga gccttggcgt tccggcggca gatgtgggca actggcctgc ggggacggtg
     4861 aatatcacgg taagcggcac taacaccgcc ggaacaacta cgacaatcac ccatccagtc
     4921 accgtcgatc tggcagcggt ggcgatctcc attaacaccg tttccggcga cgatgtgatt
     4981 aatgccgctg aaaaaagtgc agatttaacc ctttcaggca gcacctccgg cgtggaagcg
     5041 gggcaaacgg tcaccgttac ctttggcggg aaaacctaca ccgctaccgt agcgggcgat
     5101 ggtagctgga caaccaccgt acctgccgcc gatctcagcg cgttacgcga cggcgacgcc
     5161 acggtacagg ccagcgtcag cactattaac ggcaacacgg cttcggcaac ccacgcctac
     5221 agcgtcgatg ccacggcccc gacgcttgcc atcaatacca tcgccaccga cgatattctg
     5281 aacgccgccg aggcgggcaa tccgctaacc atcagcggca gcagtaacgc cgaagcgggg
     5341 cagacggtaa ccgtcacgct taatggcgtg acttacaccg gcaccgtcca ggctgacgga
     5401 agctggagcg tcagcgtacc gacggcggat ctcagcaatc tgaccgccag cccgtacacg
     5461 gtcagcgcct cggtaaacga taaagcgggt aacccggcga ccgccactca cggcctggcg
     5521 gtggatctca ctgttccagt gctgaccatc aacaccgtct ccggcgatga cattattaac
     5581 gccgccgaac acggacaggc gctggtgatc tccggctcca gcactggcgg ggaagcgggc
     5641 gatgtcatca ccgtcacgtt aaacagtaaa acctatacca ccacgctgga cgcttccggc
     5701 aactggagcg tcggcgttcc ggcggcagat gtcactgcgc tgggcagcgg cccgcaaacc
     5761 attactgcca ccattacgga tatcgcgggt aatagcgatg acgccagccg cacggtcacc
     5821 gtgaatctga ccgcgccaac cattggtatc aacactatcg ccagcgatga cgtgattaac
     5881 gccacggaaa aaagcgcgga tctgcaaatc accggcacca gtaatcaacc cgcaggcacc
     5941 acaataacgg tgacgctgaa cgggcaaaat tacaccgcga cgaccgattc ctccggcaac
     6001 tggagcgcca cggtgccagc ctcggcggtt agcgcgctgg gggaagccag ttacacggta
     6061 accgcgaacg tcaccgatag tgcaggcaac agcaattccg ccagccataa cgtgctggtc
     6121 aacagcgcgc tgcctgccgt caccattaac gcggtggcga ccgacgatat tattaacgct
     6181 gctgaatccg gaaacgcgca aaccatcagc ggccaggtca cgggcgcggc ggcgggcgat
     6241 acggtcaccg ttacgctggg cggcaacacc tacaccgcca cggtgcaggc taatttaagc
     6301 tggagtgtca gcgttccggc ggcagatatt caggccatcg gcaacggctc tctcaccgta
     6361 aacgcctcgg tgaccaacgt cgtcggcaac accggcaacg gttcgcgaga catcactatc
     6421 gatgccaatc tgcctggcct gcgggtcgat accgtggcgg gcgatgatgt tattaacagc
     6481 attgagcaca atcaggcgct ggtgatcacc ggcagcagca gcggattaac ggcgggaacg
     6541 gcgttaacgg tcgagattaa caacgttact tatggcgcga cggtattagc cgacggcacg
     6601 tggagcctcg gtattccggc ggcagacgtc agcaactggc ctgcgggtac ggtggatatt
     6661 acggtaagcg gcaccaacag cgccggaaca acctccacca ttacccatcc ggttaccgtc
     6721 gatctggctg gggtcgccat caccattaac actctctctg gtgatgatgt gatcaacgcc
     6781 gtcgaaaaag gcgaaacgct ggtcgtaagc ggcagcacca gcggtgtgga agctgggcaa
     6841 acggtgactg tcacctttgg tggcaaaaat tacaccacca ctgtggaatc taacggtagc
     6901 tggacggtga atgtgccgcc tgccgatctc gctgcgctac cggacggcgc gggcaacgtg
     6961 caggcgagcg tcagtaatat taacggcaac agcgcccagg cggatcgcgc gtatagcgtt
     7021 gatgccaccg cgccgcttgt gaccatcaac accatcgcca gcgacgatat ccttaacgtg
     7081 agcgaagcgg gcgcagggat caccatcagc ggcaccacca cggcacaggt cgggcagacg
     7141 ctgaccgtca cgctgaataa caacacgtac cagaccaccg ttcaggcgga tggcacctgg
     7201 agcgtgaatg ttccggcaac ggatttaagc ggattaaccg ccagcagtta taccgtgact
     7261 gcgacggtga gcgacaaagc gggcaacccg gcaagcgccg atcatgcgct ggcggtggac
     7321 gttaccgcgc cggatctcac cattaatacc gtcgcgggcg atgacattat caacgccatc
     7381 gaacatggtc aggcgctggt ggtcagcggc accagcacgg gcgcggcggc gggtgatgtg
     7441 gtaaccgtca cgctcaacgg caaaaactac accaccacgc tggacgcctc cggtaactgg
     7501 actgtgggca ttccggcggc ggatgtcacg gcgctggcga ccggtagcca gacgattacc
     7561 gccagcctga gcgatcgcgc gggcaacagc gacagcacta cccataatgt gaccgttgat
     7621 ctcagcggcc cgacgctgac catcagtacc gtctccgacg atgacattat taacagtacg
     7681 gagaaaaccc aggatctgac catcagcggc ggctccagcg gtctggcgac tggcaccacg
     7741 gtcaccgtga tgttgaacgg cctggcgtac agcgccacta ccgatagttc cggtaactgg
     7801 agcgttaccg tgcctgcttc ggcggtcggc gcactgggcg aggcgggata ccagattagc
     7861 gccagtgcca ccgacagcgc gggcaacaac ggcagcacca cccatacggt gaatgtcgaa
     7921 tcgctcctgc ctggggtgat cattaatacg gtggcgggcg atgacattat caatgctgct
     7981 gaaatcgctg tggcgcagac catcagcggt caggtcacgg gaacagcggt tgcggggaat
     8041 acggtgattg tcactattgg cggcaatcaa tacaccgcca ccgtgcagcc agatttaagc
     8101 tggagcgtca gcgtaccggc gaacgttttg caggcgctgg gcaacggaga actgaccatc
     8161 agcgcctcgg tgaccaattc cgcaaataat actggcaccg caacgcacga tatcgtgata
     8221 gatgccaacc tgcctggcct gcgggtcgat accgtggcag gtgatgatgt gattaacagc
     8281 attgagcaca ctcaggcgtt ggtgatcacc ggtagcggta gcggactggc ggcgggggcg
     8341 gcgttgacgg tggttatcaa tagcgtcacc tacggcgcaa cggtactcgc cgatggcacc
     8401 tggagcgtcg gtgttcctgc ggcagatgtc acaaactggc ctgcgggaac ggtcaatatt
     8461 gccgtttctg gcaccaatac cgccggaacc acaaccagca ttagccatcc ggtcacggtc
     8521 gatctcgcgg ccgtggcgat taccattaac accctttcca ctgacgatgt gattaatgcc
     8581 gccgaaaaag gcagcgattt gcagctctcc ggcaccacct ccgatgtgga agcggggcaa
     8641 accatcaccg ttatcttcgg cggcaaaagc tacaccacca cggttgccgc gggcggcacc
     8701 tgggggctga caatcccagc agccgatctc gcaaccttgc ctgacggcgc agcaaacgtc
     8761 caggccagcg tcagcaacgt cgcaggtaac agcgcccagg caacgcatgc ttacagcgtc
     8821 gatgccaccg cgccatccgt taccatcaac accatcgcca gcgacgatat tcttaacgcc
     8881 gccgaagcgg gatcggcgct gaccatcagc ggcaccagca cggcagaagc cgggcagacg
     8941 gtgaccgtga cgcttaacgg cgtcaattac agcggtaacg tgcaggcaga cggaagctgg
     9001 agcgtcagcg tcccgaccgg cgatttagct aacctcactg ccagccccta tacggtgagc
     9061 gcagcggtca gcgacaaagc cggaaatccg gcttcagcaa cacataatct gacggtggac
     9121 cttgccgctc cggttgtcac cattaacacg gtggcgggcg atgacatcat caacgccaca
     9181 gaacatgcac aggcgcagat tatcagcggc tcggcaacgg gcgcgactac cggtaacacg
     9241 gtttccgtga ccattggcac gaccacctac accaccgtgc tggacgccaa cggcaactgg
     9301 agcatcggcg tgcctgccag cgtgatttcc gcactggcgc agggcgatgt aaccattacc
     9361 gccacggtca ccgactccgc aggcaacagc ggcacggcct cgcacactgt caccgtggcg
     9421 ctcggcgctc cggtgctcgc cattaacacc atcgccgttg atgacatcat taatgccacg
     9481 gagaaaggcg cggatctggc gatttccggc agcagcaacc agcctgcggg tacgcagata
     9541 accgttacgc tcaacgggca aaattacacc accactgccg atgcatccgg taactggagc
     9601 gtgaccgttc cggcgtcacg ggtgagcgcc ctcggtgaag ccacttacac ggtgaccgcc
     9661 gccgctactg actctgatgg caacagcggt tccgccagcc ataacgtgca ggttaatacc
     9721 gcgctgccgg gcgtcaccat taacgtggtg gcaacggacg atattattaa cgccgccgaa
     9781 gcgggcgtgg accagaccat cagcgggcag gtgacgggtg ctacggcagg cgacacggtg
     9841 accgtcacgc tcggcggggc gacgtacacc gccacggtgc aggcgaattt aagctggagc
     9901 gtcgatgttc cggcagccgc tttacaggcg ctgggcaacg gcgagctgac catttcggct
     9961 tcggtaacga acagcgtagg taataccggt aacggcacgc gtgaaatcac catcgacgcg
    10021 aatctgcccg gtctgcgggt cgataccgtg gcgggcgatg atgtggtcaa tattatcgag
    10081 cacggacagg cgctggtgat taccggcagc agttccggtc tggcgacgga cagtaacgtc
    10141 acgctgacca ttaacgggca aacctatgtt gcggcggtac tggcggatgg ctcatggagc
    10201 gtcggcgttc cggcggcaga tgtcagcgcc tggcctgcgg ggacggtcac gattacggcg
    10261 agcggtagca ccacagccgg aaatccggtc agcgttacgc atccggtgac tgtcgatctc
    10321 actgcggtgg cggtcagcat caacgccatt actgccgatg acgtgattaa cgctgccgaa
    10381 aaaggcgcgg cgttaacgct ctctggcagc acttctggtg tggaggccgg gcaaacggtc
    10441 accgtcacct ttggcggcaa aacctacact gcaagcgtcg cggcgaatgg ttcctggagc
    10501 acctcggttc cggcggcaga tatggcggcc ctgcgtaatg gcgatgccag cgcacaggcc
    10561 agcgtcagca atgttaacgg caacaacgcc actactaccc acgcttacag cgtcgatgcc
    10621 agcgtgccaa cggtgaccat taacaccatt gcgggcgacg acattcttaa cgccgccgaa
    10681 gcgggtgctg cgctgactat cactggcagc agcacggcgg aagcggggca gacggtgacc
    10741 gtcaccctca atggtgcaaa ctatactggc accgtacaga cggacggcag ctggagcatc
    10801 agcgtaccgc cagccgactt aagcgccctg accgccagca actacaccgt tagcgcggcg
    10861 gtgagcgaca aagccggaaa cccggcctcg gttaatcaca acctgacggt ggatacttcc
    10921 gttccggtcg tcaccatcaa cacggtggca ggcgatgatg tgatcaacgc gacggaacac
    10981 gcccaggcgc agatcatcag cggctccgcc acgggtgcgg caaccggtag caccgtgacg
    11041 gtgactatcg gcacaaatac cttcaccacc gtgctggatg ccagcggtaa ctggagcgtc
    11101 ggcgttccgg caagcgtcgt ctcggcgctg gcgaacggca cggtgaccat taacgccagc
    11161 gtcaccgatg ccgccggaaa cagcggcagc gccacgcatc aggtgacggt caatactggc
    11221 ctgccgtcaa ttacctttaa cgccatcagc ggcgataacg tcctgaacgc cgatgaaaaa
    11281 ggccagccgt tgaccattag cggtagcagt acggggttgg cgaccggcgc acaggttacc
    11341 gtcacactca acggtcataa ctacagcgct accaccgacg cggcgggcaa ctggacctta
    11401 accgtgccgg tgagcgatct ggcggcttta ggccaggcca actatatcgt cagcgccagc
    11461 gccaccagtg cggcaggtaa caccgccagc agccaggcga atttactggt cgacagcggc
    11521 ctgccgggcg tcaccatcaa caccgtggca ggcgacgata ttatcaacgc cgccgaagcg
    11581 ggagccgctc aaaccatcag cggggtggtg actcgcgccg ccgctggcga tacggtcacc
    11641 gtgacgcttg gcggcaacac ttatactgca caagttcagg ccgatttaag ctggagcgtc
    11701 agcgttccgg cagccgacct ccaggcgctg ggcaatggtg atttgaccat taccgcctcg
    11761 gtcaccaacg cgaatggcaa caccggcagc ggcacgcggg atatcaccat tgatgccaac
    11821 ctgccggggc tgcgcgtgga tacggtggcg ggcgatgata tcgtcaacag cattgaacac
    11881 gggcaggcgc tggtgatcac cggcggcagc agtggtctga atgccggtgt gccattgacg
    11941 atcacgatta acggtacggc ttattccgcc acggtacagg cggacggaag ctggagtgtc
    12001 ggcattccgg cggcaaacgt tagcgcctgg ccagcggggg cattaaccgt tgaggtagac
    12061 gggcagagca gcgccggaaa tccggtcggc gtcagccatc cgttcactgt cgatttaacg
    12121 gcggtggcaa tcagcatcag caccgttgcc agcgatgacg taattaatgc cgccgagaaa
    12181 ggcaccaatc tgaccctttc cggcagtacc agcgggattg agagcgggca aaccgtcacc
    12241 gtcacttttg gcggtaaaac ctacactgca agcgtcgccg ccaacgggag ctggagtgta
    12301 aacgttccgg cggcagatct ggcaattctg ccagacggcg cggcgaacgt gcaggctagt
    12361 gtcagcagcg cgagcggtaa cagtgcctcg gcgacccatg cgtatagcgt tgacgccagc
    12421 gcaccgacgc tcaccattaa caccattgcc agcgacgata ttcttaacgc cacggaggcg
    12481 ggaagcccgc tcatcatcag cggcaccagc accgccgaaa ccgggcagac ggtcaccgtc
    12541 acccttaacg gcgcaaccta cagcggtaat gtgcaggcag acggtagctg gagcgtcagc
    12601 gttccccctt cagccttagg cgcgctgagc gcaagcaatt acaccgtcag cgccacggtc
    12661 aacgacaaag cgggcaaccc cggcagcgcc agccataatc tggcggtaga caccaccgcg
    12721 ccggttctca ccattaacac tgtggtgggc gatgacatta tcaacgatgc cgaacatgcg
    12781 caggcgctgg tgatctccgg caccagcacg ggcggggaag cgggcgatgt ggtgagcgtt
    12841 gtgctcaacg gcaaaaccta caccaccacc ctggatgcct ccggtaactg gagcgttggc
    12901 gttccagcgg cggatgttgc ggcactcggt agcggtgcgc agaccatcac cgccagcgtc
    12961 agcgatcggg caggcaacag cgacgacgcc agccgcaccg tgaccgtcag tctcaccgcg
    13021 ccagtgatta gcatcaacac catcgcgggc gatgatgtga tcaacgcgac ggaaaaagga
    13081 tctgatctgg cgctttccgg catcagcgat cagccagcgg gcacggcgat caccgtcacc
    13141 ctgaacggac aaaactacag cgccaccacc gactcttctg gcaactggag cgtcaccgtg
    13201 cccgcctcgg cggtcagtgc gctgggcgaa gcctcctaca gcgtgacggc gagcgtcacc
    13261 aatgctcagg gtaacagcag caccgccagc cataacgtgc aggtcaatac cgcgctaccg
    13321 ggcgtcacca ttaatccggt aacgacggac gatattatta acgccgcaga agcaggcagc
    13381 gcgcaaacta tcagcggcca ggtaaccggt gcggcggcgg gcagcaccgt caccgttgaa
    13441 ctgggcggta aaacttacac cgccaccgtc caggctgatt taagctggaa tgtgagcgta
    13501 cctgccgccg actggcaggc gctgggtaac ggtgagttaa cggttaatgc ctcggtgacc
    13561 aacgccgttg gtaacaccgg tagcggcacg cgcgatatca ccatcgatgc cagtctgcct
    13621 ggcctgcggg tggataccgt cgcgggtgat gatgtggtca atatcatcga acacagtcag
    13681 gcgcaggtga tcaccggtag cagctccggc tttgcggcgg gcacggcgct gaccgtagtg
    13741 ataaataatc aaacttacgc cgccacggtg ctggcgaacg gcagctggag cgtcggcgtt
    13801 cctgcgacgg atgtcagtaa ctggcctgcg ggaacgctga atattaccgt tagcggcgcg
    13861 aacagtgccg gaacgcaaac cagcattacc catccgctga ccgttgatct caccaccgtc
    13921 gccgtcagca taaacagcat caccagcgat gatgtgatta acgccgccga aaaaggcgcg
    13981 gcgctaacgc tctccggcag cacgtccggt gtcgaagcgg ggcaaaccgt taccattact
    14041 tttggcggca aaacctacac caccacggtg gcggctaacg gtagctggag caccaccgtt
    14101 ccggctgtgg atatggcgac cctgcgtgac ggcgatgcca gcgcccaggt gcgggtgact
    14161 aacgtcaatg gcaacagcgc cacggcaacg catgaataca gcgtcgatag cgccgcgcca
    14221 acggtgacca tcaacaccat tgccagtgat aacatcatca acgccagcga agcggcggcg
    14281 ggcgtgacgg tatccggcac cagcacggcg gagacggggc agacgctgac cgtcacgctt
    14341 aacggcacta actaccagac cacggtgcag gcagacggca gctggagttt aacgctgccc
    14401 gccagcgact taaccgcact tgccaataac ggctacaccc tgaccgcctc ggtcagcgat
    14461 ttggcgggta accctggcag cgccagcaaa ggtgtgaccg tcgataccac cgcgcccgtc
    14521 atcagtttca acaccgtggc gggcgacgat gtgattaaca acgtcgaaca cactcaggcg
    14581 cagattatca gtggcaccgc cacaggcgcg gtggcgggcg accgcctggt ggtgaccatc
    14641 gccgggcagc agtatgtcac cagcaccgat gccagcggca actggagcgt tggcgtgcct
    14701 gccagcgtga tttccggcct tgcggacggc acggtgacca tcagcgccac cattaccgat
    14761 agcgcaggca acagcagtac gcagacgcac aacgtgcagg taaacacggc ggcagtgtcg
    14821 ctctcggtca gcaccatcag cggcgataat attattaacg ccgccgaagc aggcagtgcg
    14881 ctgaccctga gcggcaccgg aacgaacttt gccgcaggta cagtagtgac cgtgttgctt
    14941 aacggcaaag gctacagcgc caccattcag agcaacggga gctggagcgt gaacgtgccg
    15001 gcggcagacg tcgcagcact cagtgatggc accagctaca cggttagcgc ctccgcgcag
    15061 gacagcgccg gaaacagcgc cacggcctca cgcagcgtgg cggtggatct caccgcgccg
    15121 gttatcagca taaacacggt ttcaacggac gaccgactca acgccgccga acagcagcag
    15181 ccgttaaccc ttaacggctc gactagcgcg gaagtggggc agacggtcac cgtcaccttt
    15241 ggcggcaaaa cctataccgc cacggttgcc gccaatggca cctgggcgct taacgtgcct
    15301 gcggcggatc tggccgcgct cgggcagggt gcgcagacca ttaccgccag cgtgaacgat
    15361 cgcgccggta accccggtca aaccacgcac gccctgaccg tcgataccgt tgcgccaacg
    15421 gtcaccatcg ccacggtggc gggtgacgat attatcaaca acgccgagca gcttgccggg
    15481 cagaccatca gcggcaccac caccgccgaa gtgggccaga cggtgaccgt tacctttaac
    15541 ggtcagagct ggactgcaac ggtcggcagc ggcggaagct ggtcggtgtt tattccggcg
    15601 cagcagtttg ccggattaag cgacggcagc tacaccatta gcgcgacggt tagcgatcag
    15661 gccggaaacc ccggcagcgc cagccgtggc gtgacgctga acggcgacgt cccctccgtc
    15721 accatcaaca cctttgcggg cgatgatgtg gtgaatgcag cggagcacgg ctcgtcgctg
    15781 gtgatcagcg gcaccaccac cgccccagtc gggcagacgc tgaccctgac cttaaacggc
    15841 aaaacctaca ccaccacggt acagaccggc ggtagctgga gctacaccct cggcagcgtc
    15901 gatgtcaccg cgctggcgga cggcaatgcc tacgtgatta acgcctcggt gagcaacgct
    15961 attggcaaca ccggcagcag caatcacacc attactgtcg atctcagcgc cccggcgatg
    16021 ggtattaata tcgattccct gcaagccgac actggcctta gctccagcga ctttatcacc
    16081 agcgtcagtc cggtggtggt caacggctcg ctcaccgccg cgcttgccag taacgagacg
    16141 gcacaaatca gtatcgacgg cggcgtaacc tggaccacgc tcaccgttac cggcacgacc
    16201 tggcgctata acgacagccg cacgctgacc gatggcagtt atctctatca ggtgcgggtg
    16261 attgacgcgg cgggcaacgt cggtgccact gacagccaga atgtggtgat cgacaccatc
    16321 gcgccagatc ccgcggtgaa aaccatcgcc atcaacgcga tcaccaccga tactggattg
    16381 attactaacg attttgtcac cagcgacacg acgcttgcgg taagcggcac gctgggggcg
    16441 gcgctttctt ccggtgagtt cgcgcaaatc agcattgatg gcggcaccac ctggcagaat
    16501 ctttcggtaa gcggtttgac ctggacatat cttgacgggc ggacgctgag cgatggcaac
    16561 tacaactatc aggtgcgggt tatcgacacc gccggaaata tcggcgcgac cgccagccag
    16621 atagtcactg tggacaccac cgcgccgctg gcgagcaaaa ccatcgccat tgcgggcatc
    16681 agcgatgaca ctgggttaag tagcagcgat tttgttactc gcgacaccac gctgaccgtg
    16741 cgcggcacgc tcggtgcggc gcttgccgcc gatgaacggg cgcaaatcag cctcgacggc
    16801 ggcgttacct ggaccacgtt aaccgttatc ggcaccagct ggagctatgc cgatggtcgc
    16861 acactgaccg acggcacctg gaactacacg gtgcgggtgg tggatctggc ggggaacgtt
    16921 gggcagaccg caacgcaaaa tgtggtggtc gacaccacca gcccggaagc ggcgaaaagc
    16981 atcaccatta ccggcatcag cgatgacacc ggagccagca gcagcgattt tattaccagc
    17041 gacaccacgc ttaccgtgcg cggcgtgctg ggggcggcgc tcggcgctaa cgagttcgcg
    17101 caaatcagta ccgacaacgg cgcaacctgg gtgaacgtaa cgctggcggc agatggcctg
    17161 aactggagtt acgttgacgg acgcacgcta acgaatggca cgacgacctg gcaggtgcgg
    17221 gtggtcgatc tggcgggcaa cgttggcgca accagcagcc agtcggcgca gatcgacacc
    17281 gttaacccgg cgcaggtgtt gaccatcgcc agcatcagca ccgacacggg gagttcggca
    17341 actgacttta tcaccagtga caccacgctc accctaaccg gttcgctggg ggcggggctt
    17401 gccagcggcg aagtggcgca gattagcctc gatggcggcg cgacctggat cacactcacc
    17461 accaacggca cgcagtggac ctacactgac agccgcacgc tgaccgacgg cagctatgtt
    17521 tatcaggtgc gggtgctgga tctggcgggg aacaccggcc cggtggtgtc gaaaacggtg
    17581 gtggtcgata cgattaaccc taccgccacg ccgaccattg tgtcgtatac cgatgatgtc
    17641 gggcagcggc aggggacatt aagcagttcg caggccaccg acgacactac gccgttgctg
    17701 aacggcgtgc tctccggccc gcttgccagc ggtgaagtgg tttacctcta ccgtaacggc
    17761 ctgctgttag gcgcggtaac gatggtcggc gcactgaact ggacctacag cgacagcggg
    17821 ctggtgagcg gtgcctacac ctacagtgcg cgagtggtgg atctggcggg gaatatcacc
    17881 gcctccagtg attttgtcct gacggtcgat acctctattc cgaccacgct ggcgcaaatt
    17941 accagccaga ccacgcgcga taccacgccg attatcagcg gggtaatcac ggcggcactc
    18001 gccagtgggc agtatgttga agtggtgatc aacggcaaaa cctacacctc tgaaccgggt
    18061 ggcgcggtag tggtcgatcc ggcgcacaac acctggtatg tacagttgcc ggataccgac
    18121 gcgctggcgg cttctgcgac cgcctataac gtcaccgcgc aggtaaaaag ctcggcgggc
    18181 aacggcaata acgccaatat tagcaacggc acggtgacgg ttaacgcggc gattgattac
    18241 acaccgacct ggaccaccgc cagcaaaacc accgcctggg gattgaccta cggccttgac
    18301 tcgcacggga tgtggacggt gctggcaaac cagcaggtaa tgcaatcgac tgacccactc
    18361 acctggtcga agaccgcgct gacgctgtat cagagcggca acaactacgc caccagctcc
    18421 atcgccgatt acgaccgtaa cggcacgggc gatctgttta tcacccgtga tgactacggt
    18481 acaggctata tcaacggctt taccaataac ggcgatggca ccttttccag cgctattcag
    18541 gtcaccgtcg gcaccctgac gtggtacggc tcgattgtgg catttgataa agagggcgac
    18601 ggctatctcg acttctggat tggcgacgcg ggcgggccgg actccaacac cttcttgtgg
    18661 aacaacgcag gtacgctggt aggtaactcc accacgtcga acagcggcgg tagcgccacg
    18721 gtgggcgggg cggtgacagg gtatctttcg ctcaacgaag gttctggtgt cgatctgaac
    18781 aatgacggca ggatcgacct ggttcagcac acctacaacc tgaacaacta ttacacgcta
    18841 tcttcgctca tcaaccaagg gaatgggacg tttgtctggg ggcagaacac caccaatacc
    18901 ttcctgagcg gggcgggcag tggcgctatg agcagcagcg tttccatgac ctgggccgat
    18961 ttcgatggtg acggcgatat ggatctcttc ctgcctgcca gccagggaag agctaactac
    19021 ggctcgctgt tattcaacac caacggcgta ctgggtagcc cggtggcggt gggcgcaacg
    19081 gcaaccacct atgccagcca gtttagcgtg gcggtggact ggaaccatga cggcctgatg
    19141 gatatcgccc gtatcgccca gaccgggcag tcgtatcttt ataccaacgt cagcaatgcc
    19201 agcaactgga cacaatcggc cctcggcggc agccagagcg gtaccaccag cggcgtggcg
    19261 gcaatggact acgactggga cggcgcggtg gatgtgctgg tgaccaaaca gtcgggcagc
    19321 gtctatctta ttcgcaacac caacacggtg agctacggca cctcgctgca cctgcgcatc
    19381 accgatccta acggcattaa cgtctattac ggcaataccg taaaactgta caactcggcg
    19441 ggagtgctgg tcgccacgca aatcatcaac ccgcagtcgg ggatgggggt taacgacacc
    19501 tcggcgctgg tcaacttcta cgggctgaat gccggagaaa cctacaacgc ggtgctgatc
    19561 aaatccactg gcaccaccgc cagcaatatc gaccagacgg tcaacaccag ctggggcggc
    19621 ttacaggcca ccgatgccac tcacgcttac gacctcagcg ccgaagcagg caccgcgagc
    19681 aacaatggca agttcgtcgg caccggctat aacgacacct tctttgccac cgcaggcacc
    19741 gacacttacg acggttccgg cggctgggtg tacagctccg gcaccggaac ctggcttgcg
    19801 aacggcggca tggacgtggt tgatttccgc ctctcgacgg tgggcgtgac cgcgaactta
    19861 agcagcaccg ccgcgcaggc caccggcttt aacacctcga catttaccaa tatcgaaggg
    19921 atatccggct cgaattttaa cgacactctg accggcagca gcggcgataa ccaactggaa
    19981 gggcgcggcg gtaacgacac gctcaacatc ggcaacggcg gccacgacac cttgctctat
    20041 aaactgctca acgcaagcga cgcgaccggc ggcaacggct cggatgtggt gaatggcttt
    20101 acggtaggga catgggaagg caccgccgac accgatcgca ttgatatccg cgagttgtta
    20161 cagggcagcg gctacaccgg caacggcaaa gccagctacg tcaacggcgt ggcaacgctg
    20221 gatgcgcagg ccggaaacat cggtgatttt gtcaaagtca cccagagcgg cagcgacacc
    20281 atcgtgcaga tcgaccgcga cggcacgggc ggcacttttg cggcaactaa cgtggtcacg
    20341 ctgacgggcg tgcataccga cctcgccact ttgctggcga atcatcagtt gatggtggtg
    20401 tagagaatga aaaaaaatgc ccagtccgtg gaagcctggc tggaggcgat gatcgccgtg
    20461 gcgcgctatt accgccttga tttttctcag gagaatgtgc gcgcgaccgt caactgggag
    20521 cgcgacagta agcgggagga gttactcacc gacatggcgc ggcagttggg gatggggctt
    20581 cggctggtgg agttctccac cgactcgctc aacccctggc ggctgccgtt gatcgccgtt
    20641 tttgataatc aacaaattgg cgtgatcacc cgccgcgata atcacgacaa tatcagcgtt
    20701 cagttcagcg gtgacgaagg gctggaaacc acgcttaacg ttacggatat tgaagataac
    20761 atcgtcgaac tggcgctgtt gcgcccgctc agcgctattc ctgacgcgcg ggtagacgac
    20821 tatattcgcc cgtatcaggc gaactggttc tggaatctct cgctaaaaga ctggcggcgc
    20881 tatggcgata ttatgctggc gtcactggtg gcgaacgtgc tggcgcttgc ggcgatgatt
    20941 ttctcgatgc aggtttacga ccgggtggtt ccggcgcagt cctacccgac gctgtgggtg
    21001 ctgttcgccg gggtgatgat ggcgatcctg tttgaattct gtatgcgcat ggtgcgcacg
    21061 cacctttccg atgtgattgg taagcgggca gatctgcgca tttcggatcg cgtttttggt
    21121 cacgcattac ggctgaaaaa taacgtgcga tcgaaatcca ccggatcgtt tatctcgcag
    21181 atccgcgaac tggaatcggt gcgggagctg atcacctcca ccactattgg tgcagtggcg
    21241 gatctgccgt tcttcctgct gtttgtcttt attttgtgga tgattggcgg ctggctggtg
    21301 ctggtggttt tgctggcgct gccgttattg gtgatccccg gcctgctggt acaacgcccg
    21361 ctggcgcggc tggcgaacga agggatgcgc gagtcggcgg tgcgtaacgc tacgctggtg
    21421 gaagccgtgc agtcgattga agacattaaa ctgctgcgcg ctgaacagcg ttttcagaat
    21481 cagtggaatc acactaacga cgtggcctcg tccatcagca tgaagcaacg ttttctcacc
    21541 gggctactgc tcacctggac gcaggaagtg caatccatcg tctacgtcgt ggtgctgttg
    21601 gtgggctgct ttatggtaat gaacggtgac atgactaccg gcgcgctggt cgggacgacg
    21661 atcctggctt cgcgcaccat cgcgccgctg tcgcagatat ccggtgtgct ctcgcgctgg
    21721 cagcaggcga aagtggcgcg caacggactt gatgaactga tgaagcgccc ggtggatcag
    21781 ccggaacacg gcaaactggt gcataaagcg gtgctacacg gcaattatca gttcagcaac
    21841 gctatctttt attacgatga agaagagaaa attgccgatg tggcgattgg caaactcaac
    21901 atccaggccg gagagaaaat cgccattctc gggcgcaacg gcgcgggtaa atcaacgcta
    21961 ctgcaaatgc tcgccggaat gcgtatcgcc cagcaggggc aggtgctact ggataacatc
    22021 agtatcgggc agcttgatcc ggcagatctg cgccgcgaca tggggctact gagccagacc
    22081 ggacgactgt ttttcggctc gctgcgggaa aacctgacga tggggatgcc ggaggccagc
    22141 gatgaagata tcgaacgcgc attaaccctc agcggggcgc tgccgtttgt gcagaagcag
    22201 aaaaacggcc tcaactacat gattcaggaa gggggatttg gtctttctgg cggtcagcgg
    22261 caaacgctgc tgctggcgcg gcttctcatc tctcaaccca atatcgtgct gctcgatgaa
    22321 cccagcgcct cgctggatga aatggcagaa gcgtatttaa tcgaacaact gaaacagtgg
    22381 attggccatc gcacgctgat tatcgccact caccgcacgg cgatgctgca actggtggac
    22441 cgcattatcg tgatggatca ggggcggatt gtgatggatg gcgcgaaaga ggccattttg
    22501 cgtgaacagg gcgaacccac gacgcgacgg gtggtgttgc aggagaagaa taaggggtct
    22561 gccgcatgaa tggacaactg caatacaacc gtggcctgac tgaaccccgc ctgccgcgtt
    22621 cggcgctggc ggtacgcgtt accgctgtga tgctgctctg ttttctcggc tgggcgtggt
    22681 acttccagct tgatgaagtc accaccggca gcggcacggt agaaccttcc gggcgcgaac
    22741 aggtggtgca gtcgctggaa ggcggaattc tgtatcatct cgacgtcaag gttggcgata
    22801 ttgttgaaca gggccagccg ctggcgcagc ttaaccgcac caaaacggaa tccgacgtcc
    22861 aggaagcaat gtcgcggctg tacgcggcgc tggcaacctc ggcgcggctg cgggcggaag
    22921 tgagtaataa accgctggtc ttccctgacg aactgaataa attccccgaa cttatcgagt
    22981 cggaaacggc gttgtacaac actcgccgcg atgggctgaa caaagccacc accgggctga
    23041 cgcaggggat tagcctggtc aaccgcgaac tggcgatgac ccagccgctg gtaaaacagg
    23101 gggcggcgag cagcgttgaa gtgctgcgcc tgcaacgcca ggccaatgaa ctggaaaaca
    23161 aactcagcga cgtgcgcacg caatactacg tacaggcgcg ggaagagtta gcgaaagcca
    23221 atgcggaagt agaaacccag cgttcggtga tccgcgggcg ggaagattct ctcacccggc
    23281 tcaactttac cgcgccggta cgtggcattg ttcaggacat tgacgtcacc acggttggcg
    23341 gcgttattgc ccccggtggt aaactgatga ccatcgtgcc gctggatgag caactgctga
    23401 tcgaggcaaa aatctccccg cgcgatgtgg cgtttatcca tccggggcaa aaatcgctgg
    23461 tgaaaataac cgcctacgat tactccatct acggcggcct gccgggagaa gtggcggtga
    23521 tatcaccaga caccgtgcag gatgaagtac ggcgcgatgt ttattactat cgggtgtaca
    23581 ttcgcacttt cagcaaccat ctggaaaaca aaagcaaaca gcaatttccg atatttcccg
    23641 gtatggtggc gacggtcgat atccgcaccg gcaaaaaaag cgtgctggat tatctgttga
    23701 aaccctttaa caaagcacag gaggcgttgc gcgaacgatg aacattagcg atgtagcaaa
    23761 aattaccggc ctgaccagca aagcaatccg cttctatgaa gagaaggggc tggtgacgcc
    23821 gccgatgcgt agcgaaaatg gttatcgaac ctacacgcag cagcatctca acgaactgac
    23881 cttactgcgc caggcgcgcc aggtgggctt taacctggaa gagagcggcg aactggtgaa
    23941 tctgtttaac gacccgcagc gtcacagcgc cgatgtaaaa cggcgcaccc tggagaaagt
    24001 ggcggagatc gaacgacaca ttgaggagct gcaatccatg cgcaaccagc tgttggcgct
    24061 ggcgaatgcc tgccctggcg atgacagcgc cgactgcccg attatcgaaa atctctccgg
    24121 ctgctgtcat catcgggcgg ggtgaaaatc agttaatccc cgtttgcacc gaaagaatat
    24181 gctgcaaact ttctaccata atgcgccccg actgaaataa gccttcctgg atttcgttgt
    24241 taacattcac agcgtgaacc agggaggcta ataatactac ttgcaagaag gcaaaaataa
    24301 acgcaccgat aatcataata tgcgtcaggg gtattttcct ttgctccttc attttaaata
    24361 acccacaaat tgaccttcct tcattaaggc gacttttcct gtgtgatgat aaacaatgac
    24421 gttatacaga cctgactgat aaacagtggc atctttattg tcttcatcaa ttgcacataa
    24481 tgaatcattg agataagcaa agtttttact gctcattcta aaggtcattc caaatccttt
    24541 ataaatggct ttctttgccg cctgggcatg ttgtgcggct tctgcatttt taaatgcctg
    24601 cgcctcggat gcacttaaaa tagccgtctg acgctgttgt tctgcgagat aacaggtgtc
    24661 atggctgacg ccctgcgcct ggcaatctgc gatgcgggaa gtgggtgaac tacagccagc
    24721 cagtaatata accgccagca gggaaaacga tttaatggag ctattcatca ttgttaacct
    24781 taaataatgt acggaaaatt gcgaataaga aattgctgtt aaaattagaa cgaatatccc
    24841 acgccgacac ccgcaccgaa atcagattgt gtatccgcag aaatacctgc tttaagtacc
    24901 gtatgcgttc cggcattaaa agatgttcct acagacaccg cagattcacc gttgaaggtt
    24961 cccgctcccg ctgacaccat catcaagtca ccggttttca cctgtggttg tgaggcgata
    25021 gcgatggctg atgcagttcc cgcattggct tctttacgat tgtcctcaac ttcgtttttc
    25081 agagaatcga aatggttatt gagcgtgtta atttgttggc tgttatctgc gacccgtttt
    25141 tgcgtgttgg ttaactgttg ctgcgtttgt gcggaatctt gtctcagact ttcaatttgc
    25201 tcatgtgcct ggacagactt ctgcgccaga ctttgcattt gtttatcgta ttgagccctt
    25261 tctttttgct gttgtgctgc cacatcggtt gtcagtgcat tcattttctg cccttgttcc
    25321 gtggtagtgc gctgattagc ctcattttga gccgccagtc tggcattaat catctcctga
    25381 taatgcgcag tattttgcgt atctccagtc gcggcgagcg ttttttgtgt ggctacgatc
    25441 tgcgcctgtt gctctgtaat ttgcgtttgc tgggcatcag ttaccgcaga ctgtcgggcg
    25501 ttgatcatgc tctgataacg ggcggttgtc tgtacatcac ctgtagcggc aacggttttt
    25561 tgtcctgcgt caggatctgt ctggacagtg ggagtctgag tggtatcagg tgtttcagaa
    25621 gggatgtagt gatgttcagt caaataggta tacgcagcgg ggctgttgtc tgtgcgttct
    25681 aaaaagcggg taatcttctg ctcaatctcc gattgcgaca tgccagagtt aatggctgaa
    25741 agctctgttg ccgccgcttc aatggtatca ccagctaaaa caacagggct ggatgttgct
    25801 gaaattatga gtgccagtaa agctacgttt acagttttca ttcattttct cttgttcaaa
    25861 ataagtcgca ttaatgtttc t
//
LOCUS       DARNVM010000061        25119 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.061, whole genome shotgun sequence.
ACCESSION   DARNVM010000061 DARNVM010000000
VERSION     DARNVM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.061"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            213..896
                     /locus_tag="SI621_002223"
     CDS             213..896
                     /locus_tag="SI621_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HEI4064942.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHLEKQQTTVINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTMNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPA
                     ETRQRKNKK"
     gene            940..6405
                     /locus_tag="SI621_002224"
     CDS             940..6405
                     /locus_tag="SI621_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEI4064943.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGVQAQAGRDNGQGVNYGQGTGTGWVAIGEDAKANSFTDTGGGSSTAVGYHSTA
                     DGRWSTALGAKTHSLGEASVALGINTTSAGERSLAIGASATSTGGFSIALGRYANSVG
                     EFSIAQGDHAETGADDAIAFGRESKALGIMSIALGATANASKEYAMALGASSAASAAN
                     AIAVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIA
                     LGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALG
                     QGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDI
                     STTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGL
                     DNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNT
                     ANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGSTD
                     AVNGSQLKTTNDNVATNTTNITNLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNV
                     KDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNA
                     TAGAFSAAHGTDATSKITNVTAGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTN
                     ITNLTDAVDSLGDDSLLWNATAGAFSAAHGTDATSKITNVKDGDLTAGSTDAVNGSQL
                     KTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAAHGTEATSKIT
                     NVKDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLW
                     NATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGSQLYGLADSFTSYLGGGADI
                     SDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDALLWDATAKGGDGAFSAGRG
                     KDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADTLGGDAEVNADGTITAPTYA
                     IAGGSYSNVGDALEAIDTTLDDALLWDATANGGNGAFSAGRGVDKTASVITNVADGAI
                     SSTSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAPSYTIANAEYNNVGDALDAL
                     DDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGEGSTDAINGSQLFNTNMLIQ
                     QNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFNDASASGIGATAVGYNAVASG
                     ESSVAIGQGSSSTVDTGIALGSSSVSSRVIVKGSRDTSVSEEGVVIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVG
                     EDSLAMGAKTIVNSNAGIGIGYGAYVDANALNGIAIGSNARANHANSIAMGNGSQTTR
                     GAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6703..8358
                     /gene="lldP"
                     /locus_tag="SI621_002225"
     CDS             6703..8358
                     /gene="lldP"
                     /locus_tag="SI621_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEI4064944.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYTAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            8358..9134
                     /gene="lldR"
                     /locus_tag="SI621_002226"
     CDS             8358..9134
                     /gene="lldR"
                     /locus_tag="SI621_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HEI4064945.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            9131..10321
                     /gene="lldD"
                     /locus_tag="SI621_002227"
     CDS             9131..10321
                     /gene="lldD"
                     /locus_tag="SI621_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HEI4064946.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVEELGKELPAALAPIAK
                     GNAA"
     gene            10369..10842
                     /gene="trmL"
                     /locus_tag="SI621_002228"
     CDS             10369..10842
                     /gene="trmL"
                     /locus_tag="SI621_002228"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEI4064947.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(10895..11716)
                     /gene="cysE"
                     /locus_tag="SI621_002229"
     CDS             complement(10895..11716)
                     /gene="cysE"
                     /locus_tag="SI621_002229"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEI4064948.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11795..12814)
                     /gene="gpsA"
                     /locus_tag="SI621_002230"
     CDS             complement(11795..12814)
                     /gene="gpsA"
                     /locus_tag="SI621_002230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEI4064949.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12814..13281)
                     /gene="secB"
                     /locus_tag="SI621_002231"
     CDS             complement(12814..13281)
                     /gene="secB"
                     /locus_tag="SI621_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEI4064950.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13344..13595)
                     /gene="grxC"
                     /locus_tag="SI621_002232"
     CDS             complement(13344..13595)
                     /gene="grxC"
                     /locus_tag="SI621_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEI4064951.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13737..14168)
                     /locus_tag="SI621_002233"
     CDS             complement(13737..14168)
                     /locus_tag="SI621_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEI4064952.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14413..15957
                     /gene="gpmM"
                     /locus_tag="SI621_002234"
                     /gene_synonym="pgmI"
     CDS             14413..15957
                     /gene="gpmM"
                     /locus_tag="SI621_002234"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEI4064953.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15967..17250
                     /gene="envC"
                     /locus_tag="SI621_002235"
     CDS             15967..17250
                     /gene="envC"
                     /locus_tag="SI621_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HEI4064954.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            17254..18213
                     /locus_tag="SI621_002236"
     CDS             17254..18213
                     /locus_tag="SI621_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEI4064955.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDMSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(18200..19234)
                     /gene="waaH"
                     /locus_tag="SI621_002237"
     CDS             complement(18200..19234)
                     /gene="waaH"
                     /locus_tag="SI621_002237"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HEI4064956.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEAATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19473..20498)
                     /gene="tdh"
                     /locus_tag="SI621_002238"
     CDS             complement(19473..20498)
                     /gene="tdh"
                     /locus_tag="SI621_002238"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEI4064957.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20508..21704)
                     /gene="kbl"
                     /locus_tag="SI621_002239"
     CDS             complement(20508..21704)
                     /gene="kbl"
                     /locus_tag="SI621_002239"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEI4064958.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21918..22850
                     /gene="rfaD"
                     /locus_tag="SI621_002240"
     CDS             21918..22850
                     /gene="rfaD"
                     /locus_tag="SI621_002240"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEI4064959.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22860..23906
                     /gene="rfaF"
                     /locus_tag="SI621_002241"
     CDS             22860..23906
                     /gene="rfaF"
                     /locus_tag="SI621_002241"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HEI4064960.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23910..24869
                     /gene="rfaC"
                     /locus_tag="SI621_002242"
     CDS             23910..24869
                     /gene="rfaC"
                     /locus_tag="SI621_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HEI4064961.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTVREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVVCRAPLMNLNNLEAAAVYKKITLI"
BASE COUNT         6306 a         6589 c         6496 g         5728 t
ORIGIN      
        1 ttgacaccga tcattaaaat ccgcgcgatc aatttattta ttggctacaa actaaaattg
       61 tttgacactt aaaaacatat tcgcacaacc cattaataca aaaattctct aacaaattta
      121 tttggcggat atgccccact ctaatcattc ataaaaaaaa tgattgtact gtaattatca
      181 acgtatcggg attatgaaaa ggaattcatc ctatgaacct aaagaaaaca ctgttaagcg
      241 tgttaataat agtgcctctc tgcttattgg cagggtgtga ctatattgaa aaagcgagta
      301 aggtcgacga cctcgttaca cagcaagagt tgcaaaaaag caaaattgag gcgcttgaga
      361 aacagcagga actcgataag cgaaagatag aacatcttga aaaacaacaa actactgtca
      421 taaatagtac caagacgctc gctggcgtgg tgaaagcggt taaagacaag caggacgaat
      481 ttgtctttac agaatttaac ccagcacaaa cacaatattt cattttaaat aacggatcgg
      541 tcggtttggc aggccgggtg ctggctattg atgcggttga aaatggcagt gttattcgta
      601 tttcgctggt taacctttta agcgttcccg tatcaaatat tggctttcat gcgacatggg
      661 gcaatgaaag acccaccgat gccaaagctc tggcaaagtg gcaacaatta cttttcaaca
      721 ccaccatgaa ttccactctg caattaatgc ctggtcaatg gcaagacatt aatctgacat
      781 taaaaggcgt ttcgcctaat aacctgaagt atctgaagtt gtccatcaat atggccaatc
      841 tccagttcga cactgttcaa cctgctgaaa ctcggcagcg aaaaaacaaa aaataaatgc
      901 agcttctgcg cttatatcaa ggaatagaga gcatcaataa tgaacaaaat ttttaaagtt
      961 atctggaatc cggcaacagg cagttacacc gttgccagtg aaacggcaaa aagccgtggc
     1021 aagaaatccg ggcgcagtaa gctgttaatt tctgccctgg ttgctggggg aatgttgtcg
     1081 tcgtttgggg tacaggctca ggcaggaagg gacaacggtc agggcgtcaa ttatggccag
     1141 ggaacaggaa caggctgggt tgctatcggt gaagatgcaa aagcaaatag ttttacagac
     1201 accgggggtg gctccagcac cgcagtaggt tatcactcaa ctgctgacgg aaggtggtca
     1261 acagccctcg gtgctaagac ccattcgctt ggcgaagcat cagtggcgtt agggattaac
     1321 actacctcag caggtgagcg aagcctcgct ataggtgcat cggcaacgtc aacgggtgga
     1381 ttttcaatcg cacttggtcg ttatgcgaac tcagtaggtg aattttctat tgcacaaggg
     1441 gatcacgcag agactggtgc tgatgacgcc atcgcatttg gacgtgaaag caaggcctta
     1501 ggaattatga gtatcgccct cggagccacg gctaatgcgt cgaaagaata tgcaatggcg
     1561 ctgggtgcaa gtagcgccgc atctgcagct aacgcgattg cagtaggccg caacagtgct
     1621 gcagctggcg tagacagcct cgcattcggt agactatccg ccgccaacgc cgcaaacgcg
     1681 attgctatgg gtgcagagag caaagctgct gaaaatgcga cggcagttgg tactaacgcg
     1741 gaggcaaatg gacttaatag tatcgcgttg ggctccggca gtattgcaga tgtcgacaac
     1801 acgattgctc tgggcaatca aagtcaggca gtagcggctg gcgcaattgc catcggtcaa
     1861 gggaataaag ctgacggcgc aaatgctatc gcgctgggta atggtagtat tacaggtggt
     1921 gtaaatgcta ttgctcttgg acaaggcagt tatgccggtt tagaaaatgg cactgcaatt
     1981 ggtgctcaag ccagtgctca agggaaaaat tcagttgctc tgggtgctgg ttctgtagcg
     2041 actgacgcgg atactgtttc tgtgggtaac acaacagctc agcgacaaat tgtcaatatg
     2101 gcagcaggtg atatcagcac taccagtact gatgccatca atggatcaca gctttatgct
     2161 atcagtaagt cagtagcgga caatcttggt gggggggcta ccgtcaatgc gcaaggcgtc
     2221 gttacttccc caaattacag gctgaaaagt ggtattttcg gcactgttgg cgacgcctta
     2281 acgggcctgg acaataatac gttacaatgg gactccctta aaaaggcata tagtgcggca
     2341 catggtacag atactaccag taccatcacc aacgttaaag acggcgctat ttctgatacc
     2401 agtaaggatg cggttaacgg ttctcagctg aaaaccacca acgataacgt agcgaccaat
     2461 actgccaata tcaccaccaa cactaacagt atcaataccc tgacggattc cgttggcgac
     2521 cttaaagacg atgccctgct gtggaatggc accgcgttca gcgccgcgca cggcaccgaa
     2581 gccaccagca aaatcaccaa cgtcaaagac ggtgacctga cggctggcag caccgacgcg
     2641 gttaacggct ctcagctcaa aactactaac gataatgtgg caaccaacac caccaacatc
     2701 accaacctga cggattccgt tggcgacctt aaagacgatg ccctgctgtg gaatggcacc
     2761 gcgttcagcg ccgcgcacgg taccgatgcc accagcaaaa tcaccaacgt caaagacggt
     2821 gacctgacgg ctggtagcac tgacgcggta aacggctctc agctgaaaac cactaacgat
     2881 gccgtggcag ccaacaccac caatatcgcc acgaatacca ccaacatcac caacctgact
     2941 gacgcagttg acagcctcgg tgacgattcc ctgctgtgga acgctacagc gggggcattc
     3001 agcgccgcac acggtactga tgccaccagc aaaatcacca acgtcaccgc tggcgacctg
     3061 acggctggca gcaccgacgc ggttaacggc tctcagctca aaaccactaa cgatgccgtg
     3121 gcagccaaca ccaccaatat cgccacgaac accactaaca tcaccaacct gactgacgct
     3181 gttgacagtc tcggtgatga ctctctgctg tggaacgcta ctgcgggggc attcagcgcc
     3241 gcacacggta ctgatgccac cagcaaaatc actaacgtca aagacggtga cctgacggct
     3301 ggcagcactg acgcggttaa cggctctcag ctcaaaacca ctaacgatgc cgtggcagcc
     3361 aacaccacca atatcgccac gaacaccact aacatcacca acctgactga cgctgttgac
     3421 agcctcggtg acgattccct gctgtggaac gctacggcgg gggcattcag cgccgcgcac
     3481 ggtaccgaag ccaccagcaa aatcaccaac gtcaaagacg gtgacctgac ggctggcagc
     3541 actgacgcgg taaacggctc tcagctgaaa accactaacg atgccgtggc agccaacacc
     3601 accaatatcg ccacgaatac caccaacatc accaacctga ctgacgctgt tgacagtctc
     3661 ggtgatgact ctctgctgtg gaacgctacg gcgggtgcat tcagtgccaa acacggtacc
     3721 aacggtactg atagcaaaat caccaactta ctggcaggca cggtatcctc tgatagcact
     3781 gacgccatta atggctcaca actttatggc ttagctgatt catttacgtc ataccttggc
     3841 ggtggtgctg atatcagtga tgcgggtgta ttaaccgggc caacctacac tattggtggt
     3901 actgactaca ataacgtcgg tgatgctctg gctgccatta acacgtcatt tagcacatca
     3961 ctcggtgacg ccctactctg ggatgcgacc gccaagggcg gtgacggtgc gtttagcgcc
     4021 ggtcgcggaa aagataacac ggccagcatc attactaatg ttgcagacgg tgcaatttcc
     4081 tctaccagca gcgacgctat taacggctca cagctctatg acaccagtaa gtacattgcc
     4141 gatactctgg gtggtgacgc agaagtcaat gctgacggca caatcaccgc accgacttat
     4201 gccatcgctg gcggcagtta cagcaacgtc ggtgacgcgc tggaagcgat cgataccacg
     4261 ctggatgacg ctctgttgtg ggatgcaaca gccaatggcg gtaatggtgc atttagcgcc
     4321 ggtcgcggag tagataaaac ggccagcgtc attactaacg tcgcagacgg tgcaatttcc
     4381 tccaccagca gcgatgctat taacggctca caactgtata ccaccaacaa gtacatcgct
     4441 gatgcgctgg gtggtgacgc agaagtcaac gctgatggtt ctattactgc gccgagttac
     4501 accattgcaa atgccgagta caacaacgtc ggtgacgccc tggatgcgct cgacgataac
     4561 gctctgctgt gggatgcaac ggccaatgac ggcgcaggtg cctacaacgc cagccatgat
     4621 ggcaaggcca gcatcatcac aaacgttgct gatggtaaca ttggcgaagg cagcactgac
     4681 gccatcaacg gttctcagct gtttaacacc aatatgctga tccagcagaa cagcgaaatc
     4741 atcaatcagc ttgccggtaa caccagcgaa acctacatcg aagataacgg tgcgggcatt
     4801 aactatgtac gtaccaacga caacggctta gcgttcaacg atgccagcgc ttcaggtatt
     4861 ggcgctacgg ctgtgggtta taacgctgtc gcctcaggcg aaagcagcgt agccattggt
     4921 caaggtagca gcagcaccgt tgataccggt attgccctgg gtagcagttc cgtttccagc
     4981 cgtgtaatag ttaaaggttc tcgtgacacc agcgtgtcgg aagaaggtgt tgtgattggt
     5041 tatgacacca cggatggcga actacttggt gcgttgtcta ttggtgatga cggtaaatat
     5101 cgtcaaatca tcaacgtagc cgatggttcc gaagcccatg acgccgttac ggttcgccag
     5161 ttgcaaaatg ccattggtgc agtcgctacc acgccgacca aatacttcca cgccaactca
     5221 acggaagaag attcactggc agtaggtgaa gactcgctag caatgggcgc gaaaactatc
     5281 gttaatagta atgcgggtat tggtatcggt tatggtgcct acgtggacgc gaatgcactt
     5341 aatggcattg ctatcggtag caacgcgcgt gcaaaccatg caaacagcat tgctatgggt
     5401 aatggctcac agacgactcg tggtgctcaa actggctaca ccgcctacaa catggacgca
     5461 ccacagaact ctgtgggtga gttctctgtc ggcagtgaag acggtcaacg tcagatcacc
     5521 aacgtcgcgg ctggttcggc tgataccgat gcggttaacg tgggtcagtt gaaagtaacg
     5581 gacgcgcagg tttcccagaa tacccagagc attactaacc tgaacaatca ggtgacgaat
     5641 ctggatactc gcgttaccaa tatcgaaaac ggcattggcg atatcgtaac caccggtagc
     5701 accaaatact tcaagaccaa caccgatggc gtagatgcca acgcgcaggg taaagacagt
     5761 gttgcaattg gttctggttc cattgctgcc gctgacaaca gcgtcgcgct gggtaccggt
     5821 tccgtggcca acgaagaaaa caccatctct gtgggttctt ctaccaacca gcgtcgtatc
     5881 accaacgttg ctgcaggtgt taatgccacc gatgcggtta acgtttcgca gctgaagtct
     5941 tctgaggcag gcggcgttcg ttacgacacc aaagctgatg gttctataga ctacagcaac
     6001 atcactctcg gtggtggtaa tggcggtacg actcgcatca gcaacgtctc cgctggcgtc
     6061 aacaacaacg acgcagtgaa ctatgcgcag ttgaagcaaa gcgtgcagga aacgaagcaa
     6121 tacaccgatc agcggatggt tgagatggat aacaaactgt ccaaaaccga aagcaagttg
     6181 agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta cactccaggt
     6241 gccagcatgg cctctattgg tggcggtact tacaatggtg aatcggctgt tgctttaggt
     6301 gtgtcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     6361 cagggtgaat actccgccgc gctcggtgcc ggtattcagt ggtaatcatc cattaactta
     6421 gtgtgactaa gggcaattct ctgatgagga ttgccctttt ctttaccaga catctccctc
     6481 caccaagaat tggccctacc aattattcgc ttatctgacc tctggttcac aattccccaa
     6541 ttaaaactca catcaatgtt gccaatgcat aacatttagt taaccattca ttgtcattat
     6601 ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg agatgagcat
     6661 cagactcatt acacgatgtg cgtggactcc aggagacctg caatgaatct ctggcaacaa
     6721 aactacgatc ccgccgggaa tatctggctt tccagcctga tagcatcgct tcccatcctg
     6781 tttttcttct ttgcgctgat taagctcaaa ctcaaaggtt atgtcgccgc ctcatggacg
     6841 gtggcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc taacgcgctg
     6901 gcctcggtga tttatggctt cttctacggt ttgtggccca tcgcgtggat cattattgcg
     6961 gcagtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat ccgctcgtct
     7021 attctttcga taacccctga ccagcgcctg caaatgctga tcgtcggttt ctgtttcggc
     7081 gctttccttg agggagccgc aggctttggc gctccggtag caattaccgc cgcattactg
     7141 gtcggtctgg gctttaaacc actgtacgcc gccggactgt gcctgattgt taacaccgcg
     7201 ccagtggcat ttggcgcgat gggtattccg attctggtcg ccgggcaggt aacaggcatc
     7261 gacagctttg agattggtca gatggtgggg cgtcagctgc cgtttatgac cattatcgtg
     7321 ctgttctgga tcatggcgat tatggacggt tggcgcggta tcaaagagac gtggcctgcg
     7381 gtcgtggttg caggtggttc gtttgccatt gctcaatacc tcagctctaa cttcattggg
     7441 ccggaactgc cagacattat ctcttcgctg gtatcactgc tctgcctgac gctgttcctc
     7501 aaacgctggc aaccagtgcg cgtattccgc ttcggtgact tgggggcgtc acaggttgat
     7561 atgacgctgg ctcacaccgg ttacaccgca ggtcaggtgc ttcgcgcctg gacaccgttc
     7621 ctgttcctga ccgccaccgt aacgctgtgg agtattccac cgtttaaagc cctgttcgct
     7681 tcgggtggtg cgctgtatga atgggtgatc aatattccgg tgccgtacct cgataaactg
     7741 gttgcccgta tgccgccagt ggtcagcgaa gcgacagcgt ataccgccgt gtttaagttc
     7801 gactggttct ctgccaccgg taccgccatt ctttttgctg ccctgctctc gattgtctgg
     7861 ctgaagatga aaccatctga cgctatcagc accttcggca gcacgctgaa agaactggct
     7921 ctgcctatct actccatcgg tatggtgctg gcgttcgcct ttatctcgaa ctattccgga
     7981 ctatcatcaa cgctggcgct ggcactggcg cacaccggcc atgcattcac cttcttctcg
     8041 ccgttcctcg gctggcttgg tgtcttcctg accggatcgg atacctcatc taacgccctg
     8101 ttcgccgcgc tgcaagccac tgcagcacaa caaattggcg tttctgacct gttgttggtt
     8161 gccgccaaca ccaccggtgg tgtcaccggt aagatgatct ctccgcaatc tatcgctatc
     8221 gcctgtgcgg cggtaggcct ggtaggcaaa gagtcagatc tgttccgctt tactgtcaaa
     8281 cacagcctga tcttcacctg tatggtgggc gtgatcacca cgcttcaggc ttatgtctta
     8341 acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag gttgccgatc
     8401 gtgtgcgggc gctgattgat gaaaaaaacc tggaggcggg catgaagttg cccgctgaac
     8461 gtcaactggc gatgcaactc ggcgtatcgc gtaattcact gcgcgaggcg ctggcaaaac
     8521 tggtgagcga aggcgtgctg cttagtcgac gcggcggcgg gacgtttatt cgctggcgtc
     8581 atgacacatg gtcggagcaa aacatcgtcc agccgctgaa aacgctgatg gccgatgatc
     8641 cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc accgcatggc
     8701 atgcagcaat gcgcgccaca ccaggcgaaa aagagaaaat tcagctttgc tttgaagcaa
     8761 cgctaagtga agacccggat ctcgcctcac aagcggacgt tcgttttcat ctggcgattg
     8821 ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc
     8881 aatcctctgt gaagcatagc cgccagcgga tgtatctggt acccccggtt ttttcacagc
     8941 tgaccgaaca acatcaggca gtcattgacg ccatttttgc cggtgatgct gacggggcgc
     9001 gtaaagcaat gatggcgcac cttagctttg ttcacaccac catgaaacga ttcgatgaag
     9061 atcaagctcg ccatgcacgc attacccgcc tgcctggtga ccataatgag cattcgaggg
     9121 agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcac aacgcattct
     9181 gccgccgttc ctgttccact atatggatgg gggtgcatat tctgaataca cgctgcgccg
     9241 caacgtggaa gatttgtcag aagtggcgct gcgccagcgt attctgaaaa acatgtctga
     9301 cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtgg cgctggctcc
     9361 ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggctgcca aagcggcgga
     9421 tgcgcatggc attccgttta ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc
     9481 tccagccatt aaacgtccga tgtggttcca gctttatgtg ctgcgcgatc gcggctttat
     9541 gcgtaacgcg ctggagcgag caaaagcggc aggctgctcg acgctggttt tcaccgtgga
     9601 tatgccgaca ccgggcgcgc gttatcgtga tgcgcattct ggtatgagcg gcccaaatgc
     9661 ggcaatgcgc cgttacttgc aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct
     9721 gaacggtcgt ccacatgatt taggtaatat ctcggcttac ctcggcaaac cgaccggact
     9781 ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat ggaaagacct
     9841 tgagtggatc cgcgatttct gggatggtcc gatggtgatc aaagggatcc tcgatccgga
     9901 agatgcgcgc gatgcagtac gttttggtgc tgatgggatt gtggtttcta accacggtgg
     9961 ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg cccgctattg cagatgcggt
    10021 gaaaggtgat atagccattc tggcggatag cggaattcgt aacgggcttg atgtcgtgcg
    10081 tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcctgt atgcgctggc
    10141 aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgaaaaag agatgaaagt
    10201 ggcgatgacg ctgactggcg cgaaatcgat cagcgaaatt acgcaagatt cgctggtgga
    10261 agagctgggt aaagagttgc ctgcggcact agctccgata gcgaaaggga atgcagctta
    10321 agggttagac gaatatctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc
    10381 gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct ttgcgctaat
    10441 accggctttc gtctgcatat catcgaacca atgggatttg cctgggacga taagcgcctg
    10501 cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactatcgc
    10561 gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct
    10621 gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc ggaaacacgc
    10681 ggcctgccag caagcattct tgatgccctg cccgctgaac aaaaaattcg cattccaatg
    10741 gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt cggtcgtggt gtatgaagcc
    10801 tggcggcagc tggggtatcc gggagcggta ttgagagatt agttactgta tgccggatag
    10861 atgattacat cgcatccggc acaatcacag gacgtcagat cccatcccca tattcaaacg
    10921 tatggttaat accgttgaaa tgctgatcca tgtccattga cggtttatcg ctgtctggtt
    10981 taccgacaat gcgtgccgga acgccagcgg cggtggtatg cggcggcacc ggttgcagca
    11041 ccacggaacc tgcgccaatc ttcgcgccgc gcccaacttc aatattgccg agaattttcg
    11101 cgcccgcgcc aatcatcaca ccttcacgaa ttttcggatg acggtcgcca ccagatttac
    11161 ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatc accgccgttt
    11221 cacccacaac gatgcccgtg gcgtggtcaa gcatgatacc gcggccaatt tttgctgccg
    11281 ggtgaatatc aacctggaac gttacagaaa cctggttttg cagaaatatt gccagtgcgc
    11341 ggcggccctg attccacagc cagtgaccga tacgataggc ctgcaaggca tgaaaacctt
    11401 tcagatataa caatggagtc gagtatttat ccaccgccgg gtcgcgggta cgtactgcct
    11461 gaatatcaca ggccgcagaa gcgatcattt ccgggtcggc ggcgtaggcc tcttccacca
    11521 cttcgcggat agcaatggca ggcataattg gcgatgacag cttgttcgcc agcatgtagc
    11581 tcagtgcact gcccaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca
    11641 ttggctcaca atccgccagc gttctggctt cggctttaat attgttccag acaatttcca
    11701 gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg
    11761 ggccgggtca tagcggtaac aaagttccct gggattagtg gctgctgcgc tcgtccttgc
    11821 gtgcacgacc taataatgtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt
    11881 gataaatttc ctcggttatt ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt
    11941 ctttcgtatt gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat
    12001 ccatgccctg accgagcatc atgccaaaac ggcggttacg cgactggttg tcggtacagg
    12061 taagcaccag atcgcccagc cccgccatgc ccataaaggt ggcaggatcg gcccccagcg
    12121 ccgcgccaag acgcgacatt tcagccagcc cacgggtgat cagcgccgta cgcgcattcg
    12181 caccaaaacc gataccgtcg gacatccccg cgccaatggc aatgacgttt ttcaccgcgc
    12241 cgccaagctg cacgccaatg aaatccgggt tgctgtaaac gcggaaactt ttgccgcagt
    12301 gcagcagctg ctggagatca tcggcaaagg tctggtcggt agaggccagc gaaatggccg
    12361 tcggtaaacc tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa
    12421 tttgatcgcc taacgcctca cgcgccacgt cctgcaacag acgcccggtt tccgcttcca
    12481 gccctttggt cgcccacacc agacgcgcat caggacgcat cagcggttta atctgacgca
    12541 gcacttcacc aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg
    12601 cagtggcgag atcgctttca agatggagcg tatcgggaaa aggcacatcg gggagaaacg
    12661 cggcgttaca gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga
    12721 caacctcgtg gccatttctt gccagggtga tggcaagagc ggtgccgtac gagccggcac
    12781 cgatcacagt cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc
    12841 ttcgccagcc tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag
    12901 gttcagttgc gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg
    12961 gaacagaatg ttcgggcagt atgcacccag gcaatgtgcc atctgggtgc cttcgatacc
    13021 cgcgatggag aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag
    13081 ggaggccgtt acggtaacac gcagtaccac ttcgtaaacg tcatctgcca gttgagaaga
    13141 tgccgtatcc agatcaagtt taacttctgg ctgccaatct ttctggaaaa cgtgcggcgc
    13201 gttcggcgct tcgaaagaaa tatccttggt ataaatacgt tggatctgga aagtcatttc
    13261 ggtgttgttt tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc
    13321 aatgtaatgc caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc
    13381 aatgcataca agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc
    13441 gtggtgcgac cgctgcgttt gatcatctct tcacgcttag cggcgttgcc atcgatcggc
    13501 aactcctgaa aactcacgcc cttgctgctc agcagtgctt tcgcacgatg gcaatacggg
    13561 caggtttctt tggtatagat ttcaacattg gccataactt agctcctgtt tttttaccct
    13621 gaaatttcat gttgcagggc ggatgcaaaa tggagtgtcc ccagaagctt ataacaataa
    13681 gtcgctgggg gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtaaattat
    13741 ttgccgcgca ccagaggtaa gttttcgcca gcccagccag caacgccctc tttcagcacg
    13801 aatacttgcg caaaaccagc tttcgtcagc gcgtttgcag gctcctggca ctgcatgcca
    13861 gaaccgtcta ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg
    13921 gctttgattt cgctcggcaa caggttgata gaacctgcga tatggccttt acggaagtca
    13981 tcacgctgac gtaaatccac aaccacagca tcttctttgt tgatcagacg cgtagcttca
    14041 ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aataaccgcc
    14101 accagtaacg cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt
    14161 tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata
    14221 cctgcgcggt gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcgcg
    14281 acgaaaaaaa agcgcaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt
    14341 cagaggctat tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat
    14401 gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat
    14461 ggctatcgtg aagaacagca agataacgcc atttttagtg ctaaaacccc ggtaatggat
    14521 gcactgtggg ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg
    14581 cctgaccgtc agatgggtaa ctccgaagta ggtcacgtta acctgggtgc cggccgcatc
    14641 gtgtatcagg acctgactcg tctggacgtt gaaatcaaag atcgcgcttt cttcgctaac
    14701 ccggtactga ctggcgcggt agataaagcg aaaaacgcag gcaaagcagt tcacattatg
    14761 ggtctgctgt ccgcaggcgg cgtacacagc cacgaagatc acatcatggc gatggtagaa
    14821 ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac
    14881 actccgccgc gcagtgctga atcctcgctg aaaaaattcg aagagaaatt tgccgcgctg
    14941 ggtaaaggcc gcgtagcgtc catcattggt cgttactacg ccatggaccg cgataaccgt
    15001 tgggatcgcg ttgaaaaagc ttatgacctg ctaactctgg cgcagggtga gttccaggcc
    15061 gataccgccg ttgctggttt gcaggctgct tatgctcgcg acgaaaacga cgaattcgtg
    15121 aaagcgaccg ttatccgtgc agaaggtcag ccagacgcgg caatggaaga cggcgatgcg
    15181 ctgattttca tgaacttccg tgctgaccgc gcacgtgaaa tcactcgtgc attcgtgaac
    15241 gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc
    15301 gaatacgccg ctgacatcaa aactgcggtt gcttacccac cagcttccct ggttaacacc
    15361 ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa
    15421 tatgcccacg ttactttctt cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat
    15481 cgcattctga tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc
    15541 gcagagctga ccgaaaaact ggttgcggca atcaagagtg gcaaatacga caccattatc
    15601 tgtaactatc cgaacggcga catggtaggt cacaccgggg tgatggaagc ggcggttaaa
    15661 gcggttgaag cgctggatca ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt
    15721 ggacaactgc tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg
    15781 ggtcaggcac acacggcaca caccaacctg ccagttccgc tgatttacgt tggtgataag
    15841 aacgtgaaag cggttgaagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg
    15901 atgggtatgg aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc
    15961 ctccccatga ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt
    16021 gcaatcaggc ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt
    16081 tccgcccacg cggatgagcg tgaccaactc aaatctattc aggcagatat cgccgcgaaa
    16141 gagcgcgcgg tacgccaaaa gcaacaacaa cgctccagcc tgcttgctca gttgaaaaag
    16201 caggaagaag cgatctcgga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat
    16261 caactgaata aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa
    16321 gccgcccagg agcgcagcct cgccgcacaa ctggatgccg cattccgtca gggcgagcat
    16381 accggtattc agctgattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct
    16441 tatttcggct atctcaacca ggcgcgacaa gaaaccattg ctcagttgaa acaaacgcgt
    16501 gaagaagtcg ctatgcaacg cgccgagctg gaagagaaac agagcgagca acaaacgctg
    16561 ttgtatgagc agcgcgccca acaggcgaag ctgacccagg cgctgaacga acgtaaaaag
    16621 acgctggcag ggctggagtc ttccatccag caaggtcagc aacagttgag cgagctgcgc
    16681 gccaacgaat cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt
    16741 gctgaacgag aagcgcgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc
    16801 aaaggcacca cctacaaacc gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc
    16861 ctgggtgcgc cgcgtggtca ggcattctgg ccagttcgcg ggccgacgct gcatcgctat
    16921 ggcgagcagc tacagggtga attacgctgg aaaggaatgg ttatcggtgc ctctgaaggt
    16981 actgaagtta aagcaattgc cgacggtcgg gtgattctgg ctgactggct gcaaggctac
    17041 ggtctggtgg tggtggttga gcatggtaaa ggcgacatga gtctttacgg ctataatcag
    17101 agcgcactgg tgagcgttgg ttcgcaggtt cgcgcgggcc agccgattgc actggtgggc
    17161 agcagtggcg gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg
    17221 gtcaatccac agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt
    17281 gcatttgccg ctctgttggc gctctcctcc cccgtaattg ctggcaaact tgccatcgtc
    17341 attgatgatt ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccttccgct
    17401 atctccgtcg ctgtattacc cgattcacca cacgccagag agatggcgac caaagcgcat
    17461 aacagcggtc acgaagtgtt gattcatcta ccgatggcac cactgagtaa acagccgctg
    17521 gagaaaaata cgctacgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt
    17581 gcggtcaata acgtgcccta tgccgtgggg atcaacaacc acatgggcag caagatgacc
    17641 tctaacctgt ttggtatgca gaaagtgatg caggcgctgg agcgttacaa tctttacttc
    17701 ctcgacagcg taaccatcgg taatacccaa gcgatgcgcg ccgcacaagg cactggcgtg
    17761 aaggtgatca aacggaaagt gttcctcgac gattcgcaaa atgaagcgga catccgtgtg
    17821 cagttcaatc gcgcaattga cctcgcgcgt cgcaacggtt caaccattgc cattgggcat
    17881 cctcaccctt caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt
    17941 acgctggtga aagccagcag cttgctgaat gaaccgcagg ttgatatgtc tacaccgccg
    18001 aaaaatactg tgcccgacac accgcgtaat ccgttccgtg gcgtgaagct ctgcaaaccg
    18061 aagaaaccga tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc
    18121 agccagagca cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc
    18181 gaagcggcaa aacttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg
    18241 accgcgtttt gtcgcgccat tgccataagc ggaaagacca cagtaacgcc tggtaaccca
    18301 ccttcacgct gcgcacatta gtaatcaggc gcttatacat accggaagta aatatctcgg
    18361 caatcatccg ttggcgggta agaatatccg gctctttgcg caccgcatgg caaacacgca
    18421 atgcttcgta agttatttgc tgatgaaatt ccggataaat cgtaattttg tcggcataat
    18481 ttcgatttaa tttctccagc aggcgggtaa tcttaatata gtgacgttga taattaaggt
    18541 ttttattccc ttgtctatgc aaccgactca ctgacgtatt atgcagataa tatttatata
    18601 atgattgctc ggtatatcgc gcacgcagcg cattaaacat gaattctgtt gtccagacaa
    18661 tatcctgatg atgtaatccg gcaataaatt taatgttatt tttaacaata acatcgcggc
    18721 gataaacccc catccagaca acgtgagtcc aacggcggga agaaagcccc atccgcagcc
    18781 agtccgggcc ggttaataca ccggttgagc gaaggcgatc gctggggatg gattgccagg
    18841 tttctcccgt ttcacgaaaa caccagtcag cgttgcactg cgccacgtcg aggtcgtcct
    18901 ctaacgccat ggtcatcagc gtttcgtaca tggtgggata cacctcatcg tcagcatcga
    18961 caaaagcgac atatttgccc gtcgccgcct cgatcccacg attacgcgcc accgatgcgc
    19021 cagcattcgc ctgatgcaac aaacgaacgt gcggatagtt ttctgcgtaa tgctttgcta
    19081 tttcaacaga attatccgtt gaaccatcgt taataataat gatttccaga gcagtccagg
    19141 tttgcgtaat taaagattcc atacaagtgc ggaaatcatc gcccgcatta tataacggaa
    19201 taataacact aagtttattg gtgctgttca tcataaatta ccgacgacct ctgaagttat
    19261 cctgacgttt tacaggcttt gtattaagaa actattaagc ctgtgagata ttatttttac
    19321 aggtgagaga gtcacttcag ggtagagaga taatgagaac agctatataa gagatcagcg
    19381 caattacgaa ctctttatta cactgacagg attttacata tttaacggat tatcctcagg
    19441 aaaaagggct ggaataccag cccttttttg tgttaatccc agctcaggat aactttcccg
    19501 gactggcccg aacgcatggc gtcaaagccc ttctggaaat catcgataga gaaacgatga
    19561 gtaatgatcg gcgaaagatc gaggccagac tgaatcagcg ccgccatctt gtaccaggtt
    19621 tcaaacatct cacgaccgta aataccttta atgaacaagc ctttaaagat cactttggtc
    19681 cagtcgatag acatatcaga cggtggaata cccagcatcg caatacgacc gccgtggttc
    19741 atggtgtcga gcatggtacg aaacgctggc ggcgcaccgg acatttccag accgacatca
    19801 aagccttcgg tcatgcccag ttctgccatc acatcattga ggttctcttt agcgacgtta
    19861 accgcacggg tgatacccat tttacgcgcc aactcaaggc ggtattcgtt aacatcagta
    19921 atcaccacgt tacgtgcacc aacgtgtttc gccaccgccg ccgccataat accaatcggg
    19981 cctgcaccag agaccagcac atcctcgccc accagatcaa acgacagcgc ggtatgcacg
    20041 gcgttaccga agggatcaaa aatggaagcc agatcatcgg aaatattgtc ggggattttg
    20101 aaagcgttga acgccggaat caccagatac tcggcaaagc agcccggacg gttaacgccg
    20161 acgccgatcg tgttgcggca cagatgggta cgaccgccac gacagttgcg acagtgaccg
    20221 caggtgatat ggccttcgcc agaaacgcga tcgccaatct tgaaaccttt cacttcctga
    20281 ccaataccta ccacttcacc gacatattca tggcccacga ccatcggcac cgggatggtt
    20341 ttttgcgacc actcatccca gttatagatg tgaacgtcag tcccgcagat ggctgtttta
    20401 cggattttaa tcagcagatc gttatgcccg agttccggta caggaacgtc ggtcatccag
    20461 atgccctctt ccgctttcag tttggataac gctttcatct cacatcctca ggcgataacg
    20521 cccagttgtt taccaatacg cgtgaatgct tctactgcac gcgtaatttg ctcaggggta
    20581 tgcgccgcag acatctgggt acgaatacgc gcctgacctt tcggaacgac cggatagaag
    20641 aaaccggtga cgtaaatccc ctctttttgc agctcacgag caaatttctg cgctactacc
    20701 gcatcgccaa gcatgaccgg aataatggcg tgatcggctc ccgccagggt aaagcccgcc
    20761 gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca ggcggtcacg cagttcgctg
    20821 cccgcttcga ccatctccag cactttgata gacgcggcaa caatggccgg tgccagcgag
    20881 ttggagaaca ggtacggacg agaacgctgg cgcagccact caaccacttc tttgcgcgcc
    20941 gccgtgtagc cgccagaagc gccacccagc gctttaccga gcgtgccggt gataatatcg
    21001 acccggccca tcacatcgca gtattcatgg gaaccacgac cgttttcacc gacaaaacct
    21061 accgcgtgtg agtcatctac catcaccagg gcatcatatt tatccgccag atcgcaaacg
    21121 cctttcaggt tggcaatcac gccgtccatc gagaacacac catcggtggc aatcagcaca
    21181 tgacgcgcac cggcttcacg cgcttctttc agacgtgctt ccagctcctg catatcgttg
    21241 ttggcataac gatagcgttt agctttgcac agacgcacac catcaataat agacgcgtgg
    21301 ttcagtgcgt cggagataat ggcgtcttcc gcgcccaaca gcgtttcgaa caggccaccg
    21361 ttagcatcaa agcaggaaga gtagagaatc gcatcttcca tccccaggaa ggccgccagt
    21421 ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa taaaacgcac cgaagccatg
    21481 ccgaaaccgt gagaatccat tcccgccttt gccgccgcaa tcagatccgg atgattcgcc
    21541 agtccgagat agttattggc acaaaagtta atgacgtggc ttccatcagc cacagtgata
    21601 tctgcttgct gcgcagacgt tataatgcgc tcttctttaa acaacccttc cgcccgtgcg
    21661 gtttccagat cgttggttaa ctgctgataa aattctccac gcattgcgat tctccagaca
    21721 gggcaaattc cagcacatat tacccaaact tataggtcgc gacgagatga cgcgttaaca
    21781 cttctgcaaa attcaggata aataacgtcc atcaatctca cttttggcga atggctgaag
    21841 ccaacgccgt gtagtgatat gatagaagta ttcgtgtctg agattgtctc tgactccata
    21901 attcgaaggt tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca
    21961 tcgttaaagc cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag
    22021 acggcaccaa gtttgtgaac ctggtggatc tggatatcgc ggactatatg gataaggaag
    22081 acttcctgat ccagattatg gctggcgaag agttcggcga tgtcgaagcg attttccacg
    22141 aaggtgcgtg ctcttccacc accgagtggg acggcaagta tatgatggat aacaactatc
    22201 aatactccaa agagctgctg cactactgtc tggagcgcga aatcccgttc ctgtatgcct
    22261 cttccgcagc cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa
    22321 aaccgttgaa tgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgtcaaatcc
    22381 tgccagaagc gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg
    22441 aaggccataa aggcagcatg gcgagcgtcg ctttccatct caacactcag cttaacaacg
    22501 gtgaatcgcc gaagctgttc gaaggtagcg agaacttcaa acgcgacttc gtttacgtag
    22561 gcgacgtggc agatgtaaac ctgtggttcc tggaaaatgg cgtttccggc atcttcaacc
    22621 tcggtactgg tcgtgcggaa tccttccagg cggtagcaga tgctacgctt gcttatcaca
    22681 agaaaggcca aatcgaatac attccgttcc cggataaact gaaaggccgc taccaggcgt
    22741 tcacgcaggc agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg
    22801 ttgccgaagg tgtaacggaa tacatggctt ggctgaatcg cgacgcataa gagctctgca
    22861 tgaaaatact ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct
    22921 atcgcacgct ccaggcgcgc tatccccagg cgataatcga cgtgatggca ccggcatggt
    22981 gccgtccatt attatcgcgg atgccggaag ttaacgaagc tattcctatg cctctcggtc
    23041 acggagcgct ggaaatcggc gaacgccgca aactgggtca tagtctgcgt gaaaagcgct
    23101 acgaccgcgc ctacgtctta cccaactcct tcaaatccgc attagtgcct ttcttcgcgg
    23161 gtattcctca tcgcaccggc tggcgcggcg agatgcgcta cggtttactt aacgatgtac
    23221 gcgtgctcga taaagaagcc tggccgctaa tggtggaacg ctatgtcgcg ctggcctatg
    23281 acaaaggcat tatgcgtaca gcacaagatc tgccgcagcc attgttatgg ccgcagttgc
    23341 aggtgagcga aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc
    23401 cgatgattgg cttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc
    23461 actatgcgga gctggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct
    23521 cggcgaaaga tcatgaagcg ggcaatgaga ttcttgccgc tttgaatacc gagcagcagg
    23581 catggtgccg gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag
    23641 cctgtaaagc cattgttact aacgattctg gtctgatgca cgttgcggcg gcgctcaatc
    23701 gtccgctggt tgccctgtat ggtccgagta gcccggactt cacaccaccg ctatcccata
    23761 aagcgcgcgt gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg
    23821 agggttatca ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg
    23881 cgctattgtt acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga
    23941 tgggcgatgt tctccatacg ctgcccgcac tcactgatgc ccagcaggca atcccaggga
    24001 ttaagtttga ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgccgccg
    24061 ttgagcgagt tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca
    24121 taaaagctga acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta
    24181 tcgacgctca ggggctggta aaaagcgcgg cgctggtgac gcgtctggcg catggcgtaa
    24241 agcatggcat ggactggcaa accgttcgag aaccgttagc cagcctgttt tacaatcgta
    24301 agcatcatat tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa
    24361 gtttgggcta tagcaaaccg caaacccagg gtgattatgc tatcgcacaa catttcctga
    24421 cgaacctgcc tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg
    24481 ataaacactg gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa
    24541 tacggattaa acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg
    24601 aaggatttgc ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtac
    24661 tggccggggc taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac
    24721 tggatagacc gaatatcacg gtttatggac caaccgatcc gggattaatt ggcggatatg
    24781 ggaagaatca ggtggtttgt agggctccgc taatgaattt aaataattta gaagcagcgg
    24841 ctgtttataa aaaaatcaca ttaatttaat aaatagtaag tgggcattat acccacttac
    24901 ttagtcaacg cttttattat tgtggttcat ttttactcaa aaacgatata gacaggaaga
    24961 ggcacacaat ccacgatgcc ggtattggtt tagcatgtgt aatgacatca ctaatgccaa
    25021 ataacataat agaagcaggg aaaaccaaga gtatatattt tttctctcgt atacagtaat
    25081 agatcattgc aacatagaac ataataagaa caagaagac
//
LOCUS       DARNVM010000062        24920 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.062, whole genome shotgun sequence.
ACCESSION   DARNVM010000062 DARNVM010000000
VERSION     DARNVM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.062"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            36..869
                     /gene="xapA"
                     /locus_tag="SI621_002243"
     CDS             36..869
                     /gene="xapA"
                     /locus_tag="SI621_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HEI4064962.1"
                     /translation="MSQARFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDADYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHTQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            929..2185
                     /gene="xapB"
                     /locus_tag="SI621_002244"
     CDS             929..2185
                     /gene="xapB"
                     /locus_tag="SI621_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HEI4064963.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVSDPDTMFWVMLIN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYVASGASLLLSAYALTLPKIPVAEKKSATSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMMMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2238..2396
                     /locus_tag="SI621_002245"
     CDS             2238..2396
                     /locus_tag="SI621_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HEI4064964.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            2437..3321
                     /gene="xapR"
                     /locus_tag="SI621_002246"
     CDS             2437..3321
                     /gene="xapR"
                     /locus_tag="SI621_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HEI4064965.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALGDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(3432..3507)
                     /locus_tag="SI621_002247"
     tRNA            complement(3432..3507)
                     /locus_tag="SI621_002247"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3472..3474),aa:Lys,seq:ttt)
     gene            complement(3512..3587)
                     /locus_tag="SI621_002248"
     tRNA            complement(3512..3587)
                     /locus_tag="SI621_002248"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3552..3554),aa:Val,seq:tac)
     gene            complement(3634..3709)
                     /locus_tag="SI621_002249"
     tRNA            complement(3634..3709)
                     /locus_tag="SI621_002249"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3674..3676),aa:Val,seq:tac)
     gene            complement(3754..3829)
                     /locus_tag="SI621_002250"
     tRNA            complement(3754..3829)
                     /locus_tag="SI621_002250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3794..3796),aa:Val,seq:tac)
     gene            4088..5503
                     /gene="gltX"
                     /locus_tag="SI621_002251"
     CDS             4088..5503
                     /gene="gltX"
                     /locus_tag="SI621_002251"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEI4064966.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(5554..5946)
                     /locus_tag="SI621_002252"
     CDS             complement(5554..5946)
                     /locus_tag="SI621_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HEI4064967.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(5948..6307)
                     /locus_tag="SI621_002253"
     CDS             complement(5948..6307)
                     /locus_tag="SI621_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HEI4064968.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            6528..6603
                     /locus_tag="SI621_002254"
     tRNA            6528..6603
                     /locus_tag="SI621_002254"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6561..6563,aa:Ala,seq:ggc)
     gene            6643..6718
                     /locus_tag="SI621_002255"
     tRNA            6643..6718
                     /locus_tag="SI621_002255"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6676..6678,aa:Ala,seq:ggc)
     gene            6926..9115
                     /locus_tag="SI621_002256"
     CDS             6926..9115
                     /locus_tag="SI621_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HEI4064969.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYNISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(9166..10368)
                     /gene="nupC"
                     /locus_tag="SI621_002257"
     CDS             complement(9166..10368)
                     /gene="nupC"
                     /locus_tag="SI621_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HEI4064970.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            10704..11942
                     /locus_tag="SI621_002258"
     CDS             10704..11942
                     /locus_tag="SI621_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEI4064971.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(12084..12410)
                     /locus_tag="SI621_002259"
     CDS             complement(12084..12410)
                     /locus_tag="SI621_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HEI4064972.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12525..13781)
                     /locus_tag="SI621_002260"
     CDS             complement(12525..13781)
                     /locus_tag="SI621_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HEI4064973.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            13985..14950
                     /gene="glk"
                     /locus_tag="SI621_002261"
     CDS             13985..14950
                     /gene="glk"
                     /locus_tag="SI621_002261"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEI4064974.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWISLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15169..15495
                     /locus_tag="SI621_002262"
     CDS             15169..15495
                     /locus_tag="SI621_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HEI4064975.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            15517..16764
                     /locus_tag="SI621_002263"
     CDS             15517..16764
                     /locus_tag="SI621_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEI4064976.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            16779..17864
                     /gene="ypdF"
                     /locus_tag="SI621_002264"
     CDS             16779..17864
                     /gene="ypdF"
                     /locus_tag="SI621_002264"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEI4064977.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     VSAHILVDSRYYADVEARTQGYQLHLLDATNTLTTIVNQIIADEQLQALGFEGQQVCW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            17864..18901
                     /gene="ypdE"
                     /locus_tag="SI621_002265"
     CDS             17864..18901
                     /gene="ypdE"
                     /locus_tag="SI621_002265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEI4064978.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            18926..21421
                     /gene="ptsP"
                     /locus_tag="SI621_002266"
     CDS             18926..21421
                     /gene="ptsP"
                     /locus_tag="SI621_002266"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEI4064979.1"
                     /translation="MLTIQFLCPLPNGIHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPQDAIARYAGQPAVLDAQCG
                     VLAINPDDAVSGYYQVAQTLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIITTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(21424..22281)
                     /locus_tag="SI621_002267"
     CDS             complement(21424..22281)
                     /locus_tag="SI621_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4064980.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVALFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22294..23028)
                     /gene="ypdB"
                     /locus_tag="SI621_002268"
     CDS             complement(22294..23028)
                     /gene="ypdB"
                     /locus_tag="SI621_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HEI4064981.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPATTVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(23043..24740)
                     /gene="ypdA"
                     /locus_tag="SI621_002269"
     CDS             complement(23043..24740)
                     /gene="ypdA"
                     /locus_tag="SI621_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HEI4064982.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5865 a         6096 c         6666 g         6293 t
ORIGIN      
        1 ggcgttagat tccaccctac aaaaaaagga tatatatgtc tcaggctcgg ttttctcata
       61 acccactgta ttgcgtagat attatcaaga cttataaacc tgatttcacg ccacgagtgg
      121 cctttatttt aggttccggg ctgggcgcgc tggccgatca gattgagaac gcggtcgcaa
      181 tttcctacga aaagctgccg ggcttcccgg taagtacggt acatggtcat gcgggggagt
      241 tagtgctggg ccatctccag ggcgtaccgg tggtatgtat gaaaggtcgc ggacatttct
      301 acgaaggtcg tggaatgacc attatgactg acgcaattcg tacctttaag ttgctgggct
      361 gcgagctact gttctgcacc aatgcggcag gctcgctgcg tccggaagtg ggggcaggca
      421 gcctggtcgc attgaaagat catatcaaca ccatgccagg tacgccgatg gtgggactta
      481 acgatgatcg ttttggagag cgtttcttct cactggcaaa tgcctatgat gcggattacc
      541 gggcgctgtt gcaaaaagtg gcgaaagaag agggattccc cctgacggaa ggtgtgtttg
      601 tttcgtatcc ggggccgaat ttcgaaacgg cggctgaaat tcgcatgatg caaattattg
      661 gcggggatgt cgttggaatg tccgttgtgc ctgaagttat ttcagcacgc cattgtgacc
      721 tgaaggtagt ggccgtttct gcgattacca atatggcaga aggcctgagt gacgtgaagc
      781 tctcgcatac ccaaacgctg gcagcagcgg agctttctaa gcagaacttt atcaacctaa
      841 tttgcgggtt tctgcgcaaa attgcctgat taaaacaaca ataaacggcc tcacgggtga
      901 ggccacatga tgcgacaagg aaacgattat gagcatcgct ttgcgcttaa aggtaatgtc
      961 ctttttgcaa tattttatct gggggagctg gctggttacc ctcggttctt acatgattaa
     1021 taccctccat tttaccggcg ctaatgtcgg catggtttac agctccaaag gtattgccgc
     1081 gattattatg cctggcatta tggggatcat cgcggacaaa tggctgcgcg ctgaacgagc
     1141 atatatgctg tgccatctgg tgtgcgcggg cgtacttttt tatgcggcgt cggtgagtga
     1201 tccggatacg atgttttggg tgatgttaat caatgcgatg gcatttatgc cgactatcgc
     1261 attatcgaac agtgtctctt actcttgcct ggcccaggca gggcttgatc cggtgacggc
     1321 tttcccaccc attcgcgttt ttggtacggt gggattcatt gtcgcgatgt gggcggtaag
     1381 cttactgcat ttggaattga gcagcctgca gctgtatgtt gcgtccggtg cgtcattgtt
     1441 gctgtcggct tatgcgctga ctttgcctaa gattccggtt gcggagaaaa aatcggccac
     1501 gtcgcttgcc agcaagctgg gtctggatgc cttcgtgctg tttaaaaatc cacgcatggc
     1561 catctttttc ctctttgcca tgatgctggg tgcggtactg caaattacca acgtttttgg
     1621 taatccgttc ctgcatgatt tcgcccgtaa cccggagttt gctgatagct tcgtggtgaa
     1681 atatccctcc attttactgt cagtttcaca gatggctgaa gtcggtttca ttttgacgat
     1741 tccgttcttt ttgaagcgtt ttggcatcaa aaccgtcatg atgatgagta tggtggcctg
     1801 gacgctgcgc tttggtttct tcgcttatgg agatccgtca ccaaccgggt ttattttgct
     1861 gctgctgtcg atgattgttt atggctgtgc attcgatttc ttcaatattt ctggttcggt
     1921 atttgtcgaa caggaagttg attccagcat tcgtgccagc gcgcaggggc tctttatgac
     1981 catggtaaat ggtgtcggcg catgggttgg ctcgattctg agtggcatgg cagttgatta
     2041 cttttcggtg gatggcgtaa aagactggca aactatctgg ctggtgtttg caggatatgc
     2101 tctttttctc gcagtgatat ttttctttgg gtttaaatat aatcatgacc ctgaaaagat
     2161 aacgcatcgt tcagttacac attaaaagga ttcgcagctc tgttccttgg ggctgctttt
     2221 atgataaagg ttaattagtg aaagatattt tattttcttc tggcgttggt tttggcattg
     2281 gtgctttgtt tacaatcgtg cgattaccta ttcctgttcc aaatgtattg ccgggtatat
     2341 tatccatcgt gtttatgtat gtcggatatc tggtggtgaa atactttatg ccatgataat
     2401 ttaatacgat gtatttatta tatggagcac ttaattatgg aacgcgtata tagaacagat
     2461 cttaagttgc tccgttattt tcttgccgta gcggaagagt tgcattttgg tcgcgcagca
     2521 gcgcgtttaa atatgtctca gcctccgctc agtattcata ttaaagagct ggaaaatcaa
     2581 cttggcacgc agctttttat tcgccattcg cgcagcgtcg tactgacaca cgcgggcaaa
     2641 atcttgatgg aagaatcgcg tcgattgctg gtgaatgcaa ataatgtatt ggctcgggtt
     2701 gaacaaatag gtcggggaga agcagggcgg attgaactcg gggttgtggg aacggcaatg
     2761 tggggacgga tgcgcccggt tatgcggcga ttcctcaggg aaaatcctaa cgttgacgtt
     2821 ctttttcgcg aaaagatgcc cgcgatgcaa atggccctgc tggaacggcg tgaactcgat
     2881 gccggaatct ggagaatggc gacagaacca ccggctggtt ttaccagctt acggttgcat
     2941 gaatcggcgt ttctggtggc gatgcctgaa gagcatcatc tctcatcatt ttccaccgtt
     3001 ccgctggaag cgctaggtga cgagtatttt gttacaatgc cgcccgtgta cactgactgg
     3061 gattttttgc agcgagtttg ccagcaggtg ggattttcac cggttgttat tcgcgaagtt
     3121 aatgaaccgc aaacggtact cgccatggtc agtatgggta ttggtatcac attgatagcg
     3181 gacagctacg cgcagatgaa ctggcccggt gtcatattcc gtccgctcaa agaacgcatc
     3241 ccggcagact tatatattgt ttatgaaacg cagcaggtga cgcccgcgct ggttaaactg
     3301 ctggcggcgt tggcgcagta gatgagaggc acgggaactg ccggacatag ttggtctgat
     3361 aagcgaagcg catcaggcaa tttcgcaaat tgcagatagc aaaaaagcgc ctttagggcg
     3421 cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
     3481 ctgctctacc aactgagcta acgacccgaa gtggtgggtg atgacgggat cgaaccgccg
     3541 accccctcct tgtaagggag gtgctctccc agctgagcta atcacccact tcggtacttc
     3601 acattgctac aagaaattta gctggcgaga aagtggtggg tgatgacggg atcgaaccgc
     3661 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcaccca cttctcaatt
     3721 tcttgctaca cggcggagac tacataaagt agttggtggg tgatgacggg atcgaaccgc
     3781 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccc cgctgtgtgg
     3841 agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa attgttcgtt
     3901 cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt tgtcaacgaa
     3961 aacaataatg cgtaaggttg caacccgaac tacattgagg aatcaggcgg gagtgataga
     4021 atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct aaacgtaagg
     4081 ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc tgcacgttgg
     4141 cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg gtgagttcgt
     4201 gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg aagccattat
     4261 ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc agaccaaacg
     4321 ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg cttataaatg
     4381 ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga aaggtgagaa
     4441 gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg atgaaccgtg
     4501 tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc agatccgtgg
     4561 tccgatcgaa ttcagcaacc aggagctgga tgatctgatt atccgtcgta ccgatggttc
     4621 tccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca cccacgttat
     4681 ccgtggcgaa gaccatatca acaacacgcc acgccagatc aacattctta aagcactgaa
     4741 agcgccggta ccggtttacg cgcacgtttc tatgatcaac ggcgatgacg gtaaaaaact
     4801 gtccaaacgt cacggggcgg tcagcgtaat gcagtatcgt gatgatggtt atctgccaga
     4861 agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg aaatcttcac
     4921 tcgtgaagag atgatcaaat acttcaccct gaatgccgtc agcaaatctg ccagtgcgtt
     4981 caacaccgac aagctgctat ggctgaacca tcactacatt aacgcgctac cgccggagta
     5041 tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc gtaacggccc
     5101 gcagctggct gatctggtga aactactggg tgaacgctgc aagacgctga aagagatggc
     5161 acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgacg ccgcgaaaaa
     5221 acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac tggccgcgat
     5281 tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg atgagctgga
     5341 agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg cagggcagtc
     5401 tccggcgctg gatgttaccg ttcacgcaat cggtaagacc cgcagtatcg agcgtatcaa
     5461 caaagcgctg gcttttattg ctgaacgcga aaatcagcag taattagcgc ataaaagata
     5521 aacggcagga gagatacccc tgccgttttt tatttattct gattcatcta tgtcgagacg
     5581 agcaaggaaa ttacgaattc cttcgcgtga caaaaattgc gcaacttttt cctgctcact
     5641 gtgagtcatg ttgtctattg cgctgatgat ttgccgataa gcaggggtgc gaatacccgg
     5701 tgcgtattcg gcaaggggtt cttcggcttc cagaagcatc ggtgtgttat ggaaggctgg
     5761 cgtgttctga ataaactcgc aaacttgcgt atcgaccaga atgagtctgg cacgaccgcc
     5821 tttcacgcca ggaaattttt ccgttttcca gcctttttct ctgatccaac ggttaacagt
     5881 ttgtttagcc acaccgaggc attcagccag ttcttcggtg gtcattttat tgcgtaatct
     5941 tttcatatta tttgctatcg cgaatcccta agcgttgtaa taatccgata atcccttccc
     6001 gcagaagcaa ggatgtaaac tgtttttgtt caactggcgt catctctttc gccagtgtca
     6061 ccagcaacac ttcaagcggc gcatcaccag acagggcagg cgcttctccc tgaccttctg
     6121 gacgttcagc attgcgaata tattcacgaa cctgttcatt gacgtgaacc agtcgggctt
     6181 tgccaccctg gacgccaggt tttggtgacg ttgtccagcc ttccttgcgt acccatttat
     6241 taatggtctg ccggctatag ccggtcagtc tggcaagttc atctggcgtc atcctctcct
     6301 tgaacataac aattccctga atgtatgtgg gtctattagt agttcatttt ataacaccac
     6361 tttacaggaa accgtgacat gaataactac tgaatgcgag cggttttcca aaattgttca
     6421 tttgcctgat ttttcagcga ttgaaactac acgttgcaat ttgccgttga cacattcggg
     6481 cggaattcat atgatgccgc ccgtcaactc gacaagcttt acgtgacggg gctatagctc
     6541 agctgggaga gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg cttagctcca
     6601 ccaaattttg cacccagcaa atttggtacg taaacgcatc gtggggctat agctcagctg
     6661 ggagagcgct tgcatggcat gcaagaggtc agcggttcga tcccgcttag ctccaccaaa
     6721 tttcaaaccc tcgctgtaaa gcgggggttt tttgtctctg ctttttgccg cttttgtaat
     6781 acagtctacg tccgggttag cgccgcctgg tgaaagcatc attggatgaa aaatcggcaa
     6841 caggctggcc cctgtttgct tcgcgatgcg aataaactta ttatttgtgt gcctgaaaac
     6901 cccgatcagt gagagtagtg tactcatgtt tgtggagcat aacctgataa aaaatatcaa
     6961 gatattcaca ctagcgttta cgctcaccgt ggtacttatt cagctatccc gttttatttc
     7021 gccacttgcc attatccatt ccagttatat ctttctggcg tggatgccac tgtgcgtaat
     7081 gctgtcaatc ttgtttatct ttggctggcg cggtgtcgtt cccgttttat gcgggatgtt
     7141 ttgcaccaat ctgtggaact ttcatctatc ttttttacag accgcggtca tgctcggcag
     7201 ccagacgttt gttgtgttgt gtgcctgcgc aatattacgc tggcagctgg ggacgcgttg
     7261 gcgttatgga ttgaccagcc gatatgtctg gcaacgtctt ttctggcttg gtttggtgac
     7321 gccgatcggc atcaaatgca gcatgtatct tgtggggagc ttttttgatt tcccgctaaa
     7381 gatatccacc tttttcggcg atgcggatgc cattttcaca gtcgttgatt tactaagcct
     7441 tttcaccgct gtgctgattt acaacatgct tttctactat ctcactcgca tgattgtaag
     7501 tccccacttt gcgcagatat tgtggcgcag ggatatcgct ccgtcgttga gcaaagagaa
     7561 acgcgcattt accttaagct ggctggcagc tcttagcgtg ctgctacttc tgatgtgcac
     7621 accgtatgag aatgacttta ttgccggtta cctggtacct gttttcttta tcatctttac
     7681 cctcggtgtc ggtaagcttc gctatccgtt tttaaatctc acctgggctg tttcaacgct
     7741 ttgcctgctg aattacaacc agaacttttt gcaaggggta ctaaccgaat attcgctggc
     7801 atttattctc gcggtgctga tttcctttag tgtttgcctg ctctatatgg tgcgcattta
     7861 tcatcgcagt gaatggctta atcgccgctg gcatttgcag gcgctgaccg atccgttaac
     7921 gctcctaccc aactttcgtg cgttagaaca agcgccggag caagaggcgg gcaagagttt
     7981 ttgctgcctg cgcattgata atcttgagtt tatgagtcgt cattacggct taatgatgcg
     8041 cgttcactgt atccgatcaa tttaccgtac gctgctgccg ttgatgcagg aaaacgaaaa
     8101 gttgtatcaa ttgccgggta gtgaactgct gttagtgctg agtggaccgg aaacggaagg
     8161 gcgactccag catatggtta acatcctgaa tagtcggcaa attcactgga ataataccgg
     8221 gctggatatg ggctatggtg ctgcctgggg gcgttttgat ggaaatcagg aaaccctgca
     8281 acccttgttg gggcagttaa gctggctggc ggagcaatcc tgcgcacatc accatgtgct
     8341 ggcgctggat agccgagagg agatggtttc cgggcagacc actaaacagg tgctattgct
     8401 gaataccatt cgtacggcgt tagatcaggg tgatttgctg ctctacgccc agccaattcg
     8461 caacaaagag ggtgaaggtt atgatgaaat cctcgcgcga ctgaaatatg acggcggcat
     8521 tatgaccccg gataagtttc tgccccttat tgctcagttt aaccttagcg cgcgttttga
     8581 tttgcaagtg ctggaatcct tgttgaagtg gctggcaaca catccttgcg acaaaaaagg
     8641 cccgcgcttt tcagtcaatt taatgccgct cacgctgctg caaaagaata ttgccgggcg
     8701 gattattcgt ctgtttaagc gttataacat ctccccgcag gcggtcattc ttgagatcac
     8761 cgaggagcag gcgttttcta acgcagaaag cagcatgtac aacatcgagc agctgcataa
     8821 gtttggtttc cggattgcga ttgatgactt tggcaccggc tatgccaact acgaacggtt
     8881 aaagcgtttg caggctgata tcatcaaaat tgatggcgtc tttgtgaaag atattgtcac
     8941 gaatacgctg gatgcgatga ttgtgagatc aattaccgat ctggcgaaag cgaagtcatt
     9001 gagtgtggtc gcggagtttg tcgagacgca acagcagcag gcgctattgc ataagctcgg
     9061 ggtgcaatat ctgcaagggt atttgattgg tcgcccgcag ccattagctg attaacaggc
     9121 gtaaaaaaaa ccggggaatt atccccggct tttttatgta tggtcttaca gcaccagtgc
     9181 tgcgattgac gcagacagca cactcaccag ggtagagccg taaaccagct tcagaccgaa
     9241 gcgagaaacc acgttacctt gctcttcatt caggccttta actgcacctg cgataatccc
     9301 gattgaagag aagttagcga aggaaaccag gaacacagag atgatgcctt cagcacgcgg
     9361 agagagtgta gaggcaattt tctgcagatc catcatcgca acgaactcgt tggaaaccag
     9421 tttggtcgcc atgatactgc ccacttgcag tgcttcactg gaaggaacac ccatcaccca
     9481 tgcaatcgga tagaagatgt agcccaggat gccctggaag gagatgctgt agccaaacca
     9541 gccagtcacg gtggcaaaca gtgcgttcag cgcggcgatc agggcgataa agccgatcag
     9601 catcgcggca acgataatgg cgactttgaa acctgccaga atgtattcac ccagcatttc
     9661 gaagaagctc tggccttcgt gcaggttgga catctggatg ttttcttcac tggcatcaac
     9721 acggtaaggg ttgatcagcg acagcacgat aaaggtgctg aacatgttca gtaccagtgc
     9781 cgcaacgacg tatttcggtt ccagcatggt catgtatgca ccaacgatgg acatcgacac
     9841 ggtggacatc gccgttgctg ccatggtgta catacgattg cgggagattt tgccgaggat
     9901 gtctttatag gcaataaagt tttcagactg acccagaatc agggagctga cggcgttaaa
     9961 ggattccagt ttgcccatgc cgttgacttt ggagagcagg aaaccaattg cgcggataat
    10021 caccggcaac acgcgaatgt gctggagaat accgatcaat gcagagataa agacgattgg
    10081 gcacagcact ttcaggaaga agaatgccag gccttgatca ttcatgctac caaagacgaa
    10141 gttagtccct tcgttggcaa atccgagcag tttttcgaac atttcggaga agcctttcac
    10201 gaagcctaaa ccaacgtcgg agttcaggaa gaaccacgcc agtaacactt cgataacaag
    10261 cagttgaata acataacgga tacgaatttt tttgcggtcg ctgcttacca gcagtgcgag
    10321 aatcgcaaca acggcaagtg ccagtacaaa atgaaggacg cggtccatat ttgctccaaa
    10381 tatgaggcag gttaaatttc cgtgcacatt ctatgtaaca tggttaagga aaacgagatc
    10441 tgacacacac tataatgacg tcctgtgaca agcttcaaaa atagtgagct gtcatacatt
    10501 tctgctatgt tgtgtatgga agctgaaagt tatgtaaatg tgctaacatt aaacataacc
    10561 ataacattca agtcatcaat cttttcgtag tcataatcct ggtctatcag agaaatcacc
    10621 acaacccatt taaatgaatt tgataatcat tctcgtttgg catagcatga aacatagcaa
    10681 aggctatgtt ttagaggcac aagatgacga actatcgcgt tgagagtagc agcggacggg
    10741 cggcgcgcaa gatgaggctc gcattaatgg gacctgcgtt cattgcggcg attggttata
    10801 tcgatcccgg taactttgcg accaatattc aggcgggtgc cagcttcggc tatcagctac
    10861 tgtgggttgt cgtttgggcc aacctgatgg cgatgctgat tcagatcctc tctgccaaac
    10921 tggggattgc caccggtaaa aatctggcgg agcagattcg tgatcactat ccgcgtcccg
    10981 tagtgtggtt ctattgggtt caggcagaaa ttattgcgat ggcaaccgac ctggcggaat
    11041 ttattggtgc ggcgatcggt tttaaactca ttcttggtgt ttcgttgttg caaggcgcgg
    11101 tgctgacggg gatcgcgact ttcctgattt taatgctgca acgtcgcggg caaaaaccac
    11161 tggagaaagt gattggcggg ttactgttgt ttgttgccgc ggcttacatt gtcgagttga
    11221 ttttctccca gcctaacctg gcgcagctgg gtaaaggaat ggtgatcccg agtttaccta
    11281 cttcggaggc ggtcttcctg gcagcaggcg tgttaggggc gacgattatg ccgcatgtga
    11341 tttatttgca ctcttcgctc actcaacatt tacatggcgg ttctcgtcaa caacgttatt
    11401 ccgccaccaa atgggatgtg gctatcgcca tgactattgc cggttttgtc aatctggcga
    11461 tgatggctac agctgcggcg gcgttccatt tttcaggtca tactggtgtt gccgatcttg
    11521 atgaggctta cctgacgctg caaccgttgt taagccatgc tgcggcaacg gtctttggat
    11581 taagcctggt tgctgccgga ctgtcctcaa cggtggtggg gacactggcg gggcaggtgg
    11641 tgatgcaggg cttcattcgc tttcatatcc cgctgtgggt gcgtcgtaca gtcaccatgc
    11701 tgccgtcatt tattgtcatt ctgatgggat tagatccgac acggattctg gttatgagtc
    11761 aggtgctgtt aagttttggt atcgctctgg cgctggttcc actgctgatt tttaccagtg
    11821 acagtaagtt gatgggcgat ctggtgaaca gtaaacgcgt aaaacagaca ggctgggtga
    11881 ttgtggtgct ggtagtggcg ctgaatatct ggttgttggt ggggacggcg ctggggctat
    11941 agttcaactg gcgtcgtacc agtcatcact ggcggatgcg gcgtaaaacg ccttatccgc
    12001 cctacgcgtt aaagagacgg tttgtaggcc tgacaagacg cgctagcgtc gcatcaggcg
    12061 ttgtgctcca ttggcgaatg tgtttaatgg cggtgtccat gtccgcggcc ttttccgcga
    12121 tgatcgtcac gatcgcgcca gccttcacga tagccacgct catacgcttt acgcttatcc
    12181 cagccacgat ggtagccatt atcatgacgc caccagcggt tcttacgcca ttcataattg
    12241 cgatgccagt agtcacggtc gcgccagtga ccaccgtccc agtaattacc gtaatgatcg
    12301 cgatcgccaa tttgtaattt gattgatggc agtagggtga tttcacctgc gtttgccgca
    12361 agcggggtaa atgccatcag ggcggccgcc agaaacagtg acctgaacat tgttattctc
    12421 cttcacgatc gaagccgtca gcggcctgtt aacgcaatat tacggggagg taaagccccg
    12481 tatcatcgcc ataactctta aatcataaag gagagcattt tttgctaaac gttatcgtgg
    12541 ggtggctgtt gcttcggacg attgaccatc atcatcggct tacctgctaa taacagccat
    12601 gccggaagca tgacgataca gagcagcggt agcaatgtgg tattgggtac aacgactgcc
    12661 gccataaaaa gacttaacca gccatcgcgt gttaccacca gcacgatgcc gagaatagcg
    12721 caggaaacgg ttattgccgc tggaacggcg gggacgtgct catgcagcat caaccctaat
    12781 gccacgccga caaacactgc cgggaagatt cgcccaccgc gaaagccact ggcggcagca
    12841 acgaccaggg cggcaagttt aattaccgcc agcaaaaagt aatcgctggt gctgaaagcc
    12901 tgatttgcca ccatctgctg catctcatcc agccctttaa acagagaaac tggtccaccc
    12961 ataaccccca gaataccgag aataaatccg ccaataccca gcacgagcac cggatttttc
    13021 atttgatgca tcatcgcgtg caaccgtggt aagcaccata cggcgaccat ccctgctgcg
    13081 atggcaatcg ccgcgacaat tgcaccgctg agaatatcgg tcatttccat ttgtccgtaa
    13141 tgcgcaatgg gcagtgaaaa atggggatgg aaaaataatc cggtagtaag tgcaccagct
    13201 gctgccgcca ttaacggcgc aaaaagacga tcccatagcg gaacttcact actaccattt
    13261 aaggtttgcg aaaatatcaa cgccgccgca acaggtgtgc caaacagcgc gccgatggtt
    13321 ccggcagagg ctaaaatagt ccactccatt cggttgacac gcggtaacag acgagcgcca
    13381 atggcaaccg caagggcgat attgacggtc atgatcggat gttctggccc caggctgacg
    13441 ccaccagcaa gaccaagaat taatgctacg ataagtcctg gtagcgcaga gggcggaact
    13501 ggtgcgccga tcagcggttc acaggcgggg tctggtccgg catgcccctg gctgaaacgg
    13561 ataaccaacc ccaccgcaat acctgttagc gttaatatgg cgatgatcca gaaaggtgaa
    13621 tcctgggcta tccccagagt tcccggcagt cgttgccaga gcaaattctg taataccgag
    13681 gcgattttca tcaccacaat cagaataaga ctggacgcaa tcccaattgc cacggcgggg
    13741 agcgataata acaacatggt tctggctcgc ggatggagca tattctattc cttatgcggg
    13801 gtcagatact tagtttgccc agcttgcaaa aaggcatcgc tgcaattggt gctgaaacga
    13861 taaagtaatt gtgtgaccca gatcgatatt tacagggagc ctgcctttcc ggcgttgttg
    13921 ttatgccccc aggtatttac agtgtgagaa agaattattt tgactttagc ggagcagttg
    13981 aagaatgaca aagtatgcat tagtcggtga tgtgggcggc accaacgcac gtcttgctct
    14041 gtgtgatatt gccagtggtg aaatctcgca ggctaagacc tattcagggc ttgattaccc
    14101 cagcctcgaa gcggtcattc gcgtttatct tgaagaacat aaggtcgagg tgaaagacgg
    14161 ctgtattgcc atcgcttgcc caattaccgg tgactgggtg gcaatgacca accatacctg
    14221 ggcgttctca attgccgaaa tgaaaaagaa tctcggtttt agccatctgg aaattattaa
    14281 cgattttacc gctgtatcga tggcgatccc gatgctgaaa aaagagcatc tgattcagtt
    14341 tggtggcgca gaaccggtag aaggtaagcc tattgcggtt tacggtgccg gaacggggct
    14401 aggggttgcg catctggtcc atgtcgataa gcgttggata agcttgccag gcgaaggcgg
    14461 tcacgttgat tttgcgccga atagtgaaga agaggccatt atcctcgaaa tattgcgtgc
    14521 ggaaattggc catgtttcgg cggaacgtgt gctttctggc cctgggctgg tgaatttgta
    14581 tcgcgcaatt gtgaaagccg acaaccgcct gccagaaaat ctcaagccaa aagatattac
    14641 cgaacgcgcg ctggctgaca gctgcaccga ttgccgccgc gcgttgtcgc tgttttgcgt
    14701 cattatgggc cgttttggcg gcaatctggc gctcaatctc gggacatttg gcggcgtgtt
    14761 tattgcgggc ggtatcgtgc cgcgcttcct tgagttcttc aaagcctccg gtttccgtgc
    14821 cgcatttgaa gataaagggc gctttaaaga atatgtccat gatattccgg tgtatctcat
    14881 cgtccatgac aatccgggcc ttctcggttc cggcgcacat ttacgccaga ccttaggcca
    14941 cattctgtaa ctccttcctg ttatatcggg aggtaactct cccgataatc ttttaaatca
    15001 tacagttaat tcaattttcc tttgtgtccc ctcacaaggc cgacctgcgt cacacttccg
    15061 tacagcggga ttaattctcc agtaaatgca ttatttgtct ggtaacggcg atttgttttg
    15121 cacgttcata atttcactcg tcaactatca ctgaacgagg aattaacgat gagtaagaaa
    15181 ctgattgctt tatgtgcctg cccgatgggt ctggctcaca cctttatggc agcccaggcg
    15241 ctggaagaag cggcggtgga agcaggctat gaagtgaaaa ttgaaactca gggcgcggac
    15301 ggcatccaga atcgcctgac cgcgcaggat atcgccgaag cgaccatcat catccactcc
    15361 gtggcggtta ccccggaaga taacgaacgt ttcgaatcac gcgacgttta tgaaatcact
    15421 ttgcaggacg caattaaaaa cgctgcgggc atcatcaaag aaatcgaaga gatgattgcc
    15481 tctgaacaac agtaatattc gacagggata attgttatgg ccattaaaaa acgcagtgca
    15541 accgttgtgc ctggcgcatc cggtgcggca gcggcagtta agaatccgca ggcctctaaa
    15601 agcagcttct ggggtgaact cccgcagcat gtgatgtcag ggatttcacg catggtgccg
    15661 accttaatta tgggcggtgt gatcctcgct ttcagccagc tgattgctta tagctggctt
    15721 aaaattcctg ctgatatcgg catcatggat gctcttaata gcgggaaatt ctccggtttc
    15781 gacctttcct tactgaaatt tgcctggctg tcgcagtcct tcggcggcgt gttgtttggt
    15841 ttcgccattc cgatgtttgc cgcttttgtg gcgaactcta tcggcggcaa actggcgttc
    15901 ccggctggtt ttattggcgg cttgatgtct acccagccga cgcaactgct gaacttcgat
    15961 cccagcacta tgcaatgggc gacctcttcg ccggtgccgt ccaccttcat tggtgcgctg
    16021 attatttcta tcgttgccgg ttaccttgtg aaatggatga accagaaaat ccagttaccg
    16081 gatttcctgt tagcgttcaa aaccactttt ttgctaccga ttctttccgc catttttgtc
    16141 atgctggcga tgtactacgt catcaccccc tttggcggct ggatcaacgg cggtatccgt
    16201 accgtgctga ctgccgcagg tgagaagggc gcgctaatgt atgcgatggg gatcgccgcg
    16261 gcgacagcaa tcgaccttgg tggcccgatc aacaaagccg caggtttcgt tgccttcagc
    16321 tttaccacgg accacgtatt accggtcacc gcacgttcta tcgctatcgt tattccgccg
    16381 attggtctgg gtctggcgac cattattgac cgtcgtttaa ccggcaaacg cctgttcaac
    16441 gctcagcttt atccgcaggg taaaaccgcc atgttccttg cctttatggg gatcagtgaa
    16501 ggtgcgattc cgtttgcgct ggaaagcccc atcaccgcca ttccgtcgta tatggtcggc
    16561 gcgattgtcg gctcaaccgc cgctgtctgg ctgggcgcag tgcaatggtt cccggaatcc
    16621 gctatctggg catggccact ggtcactaac ctgggcgtct atatggcggg gatcgcgctg
    16681 ggggcagtca ttactgcgct gatggtggtg ttcctgcgtc tgatgatgtt ccgcaaaggt
    16741 aaattgttaa tcgatagcct gtaagaagga caatcctgat gacattactc gcttcgctgc
    16801 gcgactggct taaggcgcaa caactggatg cggtgcttct ctcctcacgg cagaacaaac
    16861 agccgcatct ggggatctcc accggatcag gctatgtggt gattagccgt gtaagtgcgc
    16921 acattctggt ggattcgcgc tactacgcgg atgtcgaagc ccgcacgcaa ggctaccagc
    16981 tgcatttgct tgacgcgacg aacacgctta ccactatcgt caatcaaatc attgccgatg
    17041 agcagttgca agcgctcggt tttgaaggcc agcaggtatg ttgggaaacc gcgcaccgct
    17101 ggcagtctga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg cggcaaatca
    17161 aaacgccaga ggaggtggag aaaatccgcc tcgcctgtgg gattgccgat cgcggtgctg
    17221 agcatattcg ccgctttatt caggcgggga tgagcgagcg cgagatagcc gctgaactgg
    17281 agtggtttat gcgccagcag ggcgcagaaa aagcctcttt cgataccatt gtcgccagcg
    17341 gctggcgtgg ggcgctgccg cacggcaaag ccagcgacaa gattgttaca gcgggcgagt
    17401 ttgtcactct cgatttcggt gcgctgtatc agggctactg ctctgatatg acgcgcacct
    17461 tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac gtctatcaaa
    17521 ttgtcctgca ggcacagctc gcagcaatct ccgcgattcg ccccggcgtg cgctgccagc
    17581 aggttgacga cgccgcgcgc cgggtcatta cagaagcagg ttatggcgac tatttcggtc
    17641 ataacaccgg tcatgctatc ggcattgaag tccatgaaga gccgcgtttt tcaccgcggg
    17701 ataccacgac gctacagcca ggcatgttac tgaccgtgga gccggggatt tatttgccag
    17761 ggcaaggggg cgtgcgcatc gaagatgttg tgctggtcac cccgcaaggc gcagaagtgc
    17821 tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt tatcgttatt
    17881 aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtgc ggcagatcct
    17941 gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gacggcctgg gatcggtgct
    18001 gatccgcctc aatgaatcga caggtccgaa ggtgatgatc tgtgcgcata tggatgaagt
    18061 gggatttatg gtgcgcagca tctcccgcga aggggcgatc gatgtgttgc cggttggcaa
    18121 tgtacgcatg gctgcccgcc agcttcagtc ggtgcgtatc actacccgtg aagagtgcaa
    18181 aattccaggc ctgcttgacg gcgaccggca ggggaatgac gtcagcgcca tgcgcgtgga
    18241 cattggtgcg cgctcgtatg acgaagtgat gcaggcggga attcgtccag gcgatcgcgt
    18301 cacctttgat acaacttttc aggttctccc tcaccagcgg gtgatgggga aagcctttga
    18361 tgatcgcctc ggttgctatc tgctggtgac gttactacgt gagctgcacg acgccgaact
    18421 gcctgcggag gtgtggctgg tggccagctc cagcgaagag gtgggattac gcggcgggca
    18481 aaccgctacc cgtgcggtgt cgccggacgt tgcgattgtg ctggataccg cctgctgggc
    18541 gaaaaacttt gattatggcg cggctaacca tcgccagatt ggtaacggcc cgatgctggt
    18601 attaagcgac aagtcgctga ttgcgccgcc aaaacttacc gcgtggattg aaaccgtggc
    18661 ggcagaaatt ggcgtgccgt tacaggcaga tatgttcagc aacggtggca cggacggtgg
    18721 ggcggtgcac ttaaccggca ctggcgtacc cacagtggtg atggggcctg ccacccgcca
    18781 cggacattgc gcggcatcga ttgccgattg tcgcgacatt ttgcagatgc agcaactttt
    18841 atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg atttcagatg
    18901 atctcctgat taacccggag cggttatgct aacgattcaa tttctctgtc ctctgcctaa
    18961 cggtattcat gctcgtccgg cgtgggaact taaagaacag tgcagccagt ggcaaagcga
    19021 aatcaccttc atcaaccatc gccagaacgc aaaggcagat gccaaaagct cgctggcgct
    19081 gattggcact ggcactttat ttaatgacag ttgcagcctg aacattagcg gcagcgatga
    19141 agagcaggcg cggcgcgtgc tggaagagta catccaagtg cgctttatcg acagcgacag
    19201 cattcagccg acgcaggcag aactgacggc gcatccactg ccgcgttcat taagccgtct
    19261 gaatcctgat ttactgtacg gcaatgtgct ggcaagcggc gtcggcgtgg gtacgctgac
    19321 cctgttacag agcgacagcc tcgatagtta tcgggtaatc cctgccagtg cgcaagactc
    19381 cacccggctg gagcacagcc tggcaacgct tgccgagcaa ctgaaccagc agctgcgtga
    19441 gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc aggatgatga
    19501 atttgcaggc aatatccgtc gccttatggc agaacagcat cagggactgg gggcggcgat
    19561 catcagcaat atggagcagg tttgcgccaa actatctgct tctgccagcg attatctgcg
    19621 cgagcgggtc agcgacattc gcgatatcag cgaacagttg ctgcatatca cctggccgga
    19681 actgaagccg cgcaacaatc tggtgctgga aaaaccgact attctggtgg ccgaggattt
    19741 aaccccaagc cagtttttga gcctcgattt aaaaaatctt gcgggcatga ttctggagaa
    19801 aaccgggcgt acctcgcata ccctgattct ggcccgtgcc tcggcgatcc cggtactgag
    19861 tggcttgccg caggatgcga tcgcgcgcta tgccgggcaa ccggcagtgc ttgacgccca
    19921 gtgcggcgtg ctggcgatta atcctgatga cgcggtgagc ggttattatc aggttgcgca
    19981 gacgctggca gataaacgtc agcagcagca ggcgcaggct gccgcgcagc tggcctattc
    20041 ccgtgataac aagcgcattg atatcgcggc gaatattggc accgctctgg aagcgcctgg
    20101 cgcgtttgcc aacggcgcgg aaggcgtcgg gctgttccgt accgaaatgc tctatatgga
    20161 tcgcgacagc gcgccggacg agcaggagca atttgaagcc taccagcagg tgctactggc
    20221 agcgggcgac aagccgatta tcttccgcac gatggacatc ggcggcgata aaagcattcc
    20281 ttatctcaac attccccagg aagagaaccc gttcctcggc tatcgcgcgg tacgtattta
    20341 cccggaattt gctggcctgt tccgcactca actgcgggcc attttgcgcg ccgccagttt
    20401 cggcaacgcc cagttgatga tcccaatggt tcacagcctc gatcagatct tatgggtgaa
    20461 gggcgagatc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc atgcagagac
    20521 gattacgctg ggtatcatgg tggaagtgcc gtcggtgtgc tacatcatcg accacttctg
    20581 cgatgaggtc gatttcttca gtatcggttc taacgatatg acccagtatc tgtatgcggt
    20641 cgatcgtaat aacccgcgcg tatcgccgct gtataacccg attacgccat cgttcctgcg
    20701 catgttgcag cagattatta ccactgcgca tcagcgcgga aaatgggtag gtatttgcgg
    20761 tgaactgggc ggtgaaagcc gttatctgcc gctactgctt gggctgggcc tggatgagct
    20821 gagtatgagt agcccgcgca ttccggcggt gaaaagccag cttcgtcaac tggatagcga
    20881 ggcgtgtcgg gaactggcgc gtcaggcgtg tgaatgtcgc agcgcgcagg agattgaagc
    20941 gttactcacc gcctttacgc cggaagaaga cgttcgcccg ctgctggcgc tggagaatat
    21001 cttcgttgat caggccttta gcaataaaga gcaggcgatc cagttcctgt gcggtaacct
    21061 cggcgttaac gggcgcactg aacatccgtt cgagctggaa gaggatgtct ggcaacggga
    21121 agagattgtt accaccggcg ttggttttgg cgtagcgatc ccgcacacca aatctcagtg
    21181 gatccgtcat tccagtatca gcattgcccg gctggtgaaa ccggttgact ggcagtcaga
    21241 aatgggcgaa gtcgaactgg tgatcatgct gacgttgggt gctaacgaag ggatgaacca
    21301 tgtgaaagtc ttctcgcagc tggcgcgcaa actggtgaat aaaaacttcc gccagtcgct
    21361 gttcgccgcg caagatgcac aaagcatcct gacgctgctg gaaacagaat taaccttctg
    21421 acgttagccc tgaaaacggg cgctgtactc tcctggcgtc aggccaaact gacgccggaa
    21481 gacgcgacaa aaatagtcgc tgtccggaaa gccgcaacgc tgcgccactt catgaatcga
    21541 cagatgatat ttctgcaaaa tcatcctcgc cttcgccatt cgcacccaac gcacatattc
    21601 gataaaacgc attgttccat gctgcgcaaa cagttttgac agatgattgg gcgtgatatt
    21661 aaaaaacagc gccacgctct cacgggtgag cggctgggca taattatcct gaacccagtt
    21721 acaaatgctg tgataaagaa aatcgccgcg cggctgcgaa ttacccggcg gcatattcac
    21781 cacgttgcgg caaagatgca gcaaacttaa caccagcggc tgaatgatat tctgctccag
    21841 cggcgaacgg ctaagatggg tgagggcagt aagcattgct tcaccttcgc cgcgttgcag
    21901 gctgggaagt tggatctggc gagcaggatg caacaacgac gtggtacggc tatcgtaaaa
    21961 cgataaccca agccaggtcg gtgcgaacac caggcttaac agcatcaccg gtttgttgtt
    22021 gatcggtaaa ttagccgcgc gcgctggaat aaacagcatt tctccttcaa ccatacgttg
    22081 ctgctttgct tccagcagat tgccgtattc gccgcgcagc acgatatcca gacggggaaa
    22141 atcaatacat aaactgccca ccggtaacga ggcaggctgg ctggcaaacc agacgcgccc
    22201 cagtagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt gttgatcggc
    22261 gggcaagcct ggtgctttca tgaaagttcc cgattaaaga tgcattaact ggcggaattc
    22321 tttaactttg ctgcggctga ctggcacttc aaaatccaga tctttcagcc gcagaatata
    22381 ggtgttatta aaccacggtt cgatctcgcg gattttgttc agattgacac aaaatgagcg
    22441 atggcagcgg aaaaaatgcg atggcggcag cttgctgcaa aactcggtaa tgttcatcgg
    22501 cattacgtag gattcacgcc gcgtatagac aaaggtcatt ttctcatgcg cttcggcgta
    22561 atagatatcg ttaattggcg tgacgatgat tcgctcatct ttcaccagat taatggtgtc
    22621 attctcacgc gttaccgtcg tggcaggcgt actgctggtc tgctgttgtt gccaggctgc
    22681 ttccagtttt tgtagcatcc cggtaatgcg tgactcctga tacggtttga gaatgtagtc
    22741 aaacgcctcc agttcaaacg cttctaccgc atgttctttc catgcggtga taaacacaat
    22801 aaacggttta tgggcgaact ggctgatgtt ttgcgccagc aacacgccat ccagcgacgg
    22861 aatattgata tccagaaaaa tggcgtcgac gcggttatgc tgcaaaaact tcaacacgtc
    22921 cagaccgtcg tcaaaggtgc cgacaatctc catctggctg tgctctttaa ttagccagct
    22981 cagttcctgt tgtgccagga attcgtcttc aacaatgatg actttcacaa tatcactccg
    23041 gctcaaagca ataacgtagc ctgtgaggcg actggggtgc gttggttagg aatgtaaaac
    23101 gcaatttccg ttcctggttc caggcggcgg atatgcagcc cctcgccata caataacttc
    23161 acgcgatgat ggacattcag caggccaatt ttattgcccg gcatttcgtt cgcttcgacc
    23221 cgctcaatga cttttggatc gatgccgtgc ccggtatctc gcaccgcaat acgtacccga
    23281 ttaccgcact ctgcaacgct gatggtgaca acgcctttac ctttgcaagg ctggataccg
    23341 tggacaatgg cgttttccac caacggctgg atcagcaggc tgggaatgca gcaattcacc
    23401 tcttcatcaa tatcatagat aaccgtcagc ttgtcgccaa agcgggcctg ctcaatggca
    23461 atatagtctt taatttgata cagctctttt ttgatatcga tttgctcatc gtcttttaat
    23521 tcaatgttat agcgcagata acgcgacaga ttaaagatca gctggcgagc ggtatccgga
    23581 ttcagacgga ttgacgatga aatagcgttc aaagcgttaa acaggaaatg gggattaatt
    23641 ttgctttgca gggcgcgaag ctctgcctta tttgccattt cacgtagctg ctcggcgcgt
    23701 gaaacctcca gttgggtgga gatgatttgc gacagaccga ccgccatttc ctgtaatgac
    23761 gaggtgatct gatgcgcgtg gcagtagtaa attttcagcg ttccggtcac aacgcctttc
    23821 tcccacaatg ggatcaccag catggaatga atctctggtg tgcggtgggc ttcatcattg
    23881 tttttaatga tgatttttcc gtaattcatc gcctgtcggg tagtcgggct aatgaagtca
    23941 tcgccattct gatagttgtg ttcacccacg ccgacatagg ccagcacatg atcggtatta
    24001 gtaatcgcca ccgcatcggc gtgaatatca tcgcgaataa tttcacaaac tttgcgtaat
    24061 gactcgctat tgacatggcg aaacagcggt agcgttttgt tggcgatatc cagcgccagc
    24121 ttggcctgcc gcgcggcgct ggcctctttt tcgccctcaa cgctttgcac cagcagcaca
    24181 ataaagccga tacagacgct accgaggatc attggaatcc cgattttaga gacgatatcg
    24241 atccccaatg cggtagttgg tgcccagaca atcaccagaa tcatggtcaa cgtttcacac
    24301 aacatgccgc cgagaatacc gacgcgccag cgctgtgctt tggggatttt caggttgatc
    24361 cagcccgata tacaacccgc cagaatgctg gtgataaagc aggggatcgc cgtcacgccg
    24421 ccgatatcaa ttaaataacg gtgaatacct gcgatcacgc cggtaataat gcctacccac
    24481 gggccaaaca gaattccgcc tgacatcacc gcgataatac gtacgttcac cagcgagcct
    24541 tctacgggaa cgccggacca ggtgctgaac agggcgaaca gcgagaaaat ggcggtaacg
    24601 gcgagcagct ctttcggtga gtgtgccgac ttgtgcaaca gttcgcgaaa caggcggata
    24661 cggatgagaa agaacaggca gataagcatt aacgctgccc gatcgaagac cgccagcagc
    24721 atgttgaata tttcgtgcac gggtgactct gaataaggat taaacctcta tgataggtaa
    24781 cctgaaggct gatgaccagc aggccgtttt tgaggaaaaa ccacaatttt aaaaggggat
    24841 tgaaagataa atggtgtatt agcaatgcat ctcttgataa tgcacatatg cttggtaaat
    24901 ttattgaaat tataatgtta
//
LOCUS       DARNVM010000063        24544 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.063, whole genome shotgun sequence.
ACCESSION   DARNVM010000063 DARNVM010000000
VERSION     DARNVM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.063"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            231..437
                     /locus_tag="SI621_002270"
     CDS             231..437
                     /locus_tag="SI621_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064983.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            990..1484
                     /locus_tag="SI621_002271"
     CDS             990..1484
                     /locus_tag="SI621_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HEI4064984.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1484..3586
                     /locus_tag="SI621_002272"
     CDS             1484..3586
                     /locus_tag="SI621_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEI4064985.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSLGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVPTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3583..3795
                     /locus_tag="SI621_002273"
     CDS             3583..3795
                     /locus_tag="SI621_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4064986.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3795..5303
                     /locus_tag="SI621_002274"
     CDS             3795..5303
                     /locus_tag="SI621_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEI4064987.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYETDGNDSKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     VSGVIRLPRDLDRYSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5248..7275
                     /locus_tag="SI621_002275"
     CDS             5248..7275
                     /locus_tag="SI621_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEI4064988.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDIPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7362..7685
                     /locus_tag="SI621_002276"
     CDS             7362..7685
                     /locus_tag="SI621_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HEI4064989.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVRVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNA"
     gene            7678..7953
                     /locus_tag="SI621_002277"
     CDS             7678..7953
                     /locus_tag="SI621_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HEI4064990.1"
                     /translation="MPDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7965..8543
                     /locus_tag="SI621_002278"
     CDS             7965..8543
                     /locus_tag="SI621_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4064991.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8540..8941
                     /locus_tag="SI621_002279"
     CDS             8540..8941
                     /locus_tag="SI621_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4064992.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQALDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8952..9695
                     /locus_tag="SI621_002280"
     CDS             8952..9695
                     /locus_tag="SI621_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4064993.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            9756..10142
                     /locus_tag="SI621_002281"
     CDS             9756..10142
                     /locus_tag="SI621_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEI4064994.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10151..10480
                     /locus_tag="SI621_002282"
     CDS             10151..10480
                     /locus_tag="SI621_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEI4064995.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10452..13517
                     /locus_tag="SI621_002283"
     CDS             10452..13517
                     /locus_tag="SI621_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEI4064996.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSQKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13517..13846
                     /locus_tag="SI621_002284"
     CDS             13517..13846
                     /locus_tag="SI621_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4064997.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13856..14554
                     /locus_tag="SI621_002285"
     CDS             13856..14554
                     /locus_tag="SI621_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEI4064998.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14560..15303
                     /locus_tag="SI621_002286"
     CDS             14560..15303
                     /locus_tag="SI621_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4064999.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASIFV"
     gene            15240..15848
                     /locus_tag="SI621_002287"
     CDS             15240..15848
                     /locus_tag="SI621_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEI4065000.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQVVLGAALIAVA
                     WWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15909..19388
                     /locus_tag="SI621_002288"
     CDS             15909..19388
                     /locus_tag="SI621_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEI4065001.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPYLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRMSITDVNN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PVFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGARHERENSDTYSSCRLI
                     VKKNGAEIYNRTALDNTLIYTGVIDMPAGSGVMTLEFSVSAWWVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            19447..21843
                     /locus_tag="SI621_002289"
     CDS             19447..21843
                     /locus_tag="SI621_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HEI4065002.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEEMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAAASQT
                     ASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQNAA
                     AESESAAAGSATSAAGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKASETAAKS
                     SQDAAAESESAAAGSASAAAASATASANSQKAAKTSETNAKTSETAAANSAQASAASQ
                     TAAKASEDAAREYASQAAEPYKQVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDDE
                     ITMPGDKVVKFKRASKATYINKSGVLTEAAIDEPRFERDGLLIEGQRTNYMLNSESPA
                     SWGRSSNMDVPETGTDSFGFTYGKFVCNDSLIGQTSAINMASIAATKSVDVSGDNKYV
                     TTSCRFKTELQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGASGRITARVRKD
                     ETTGWIFAEATIQAIDGELKIGSQIQYSPKQGGATVSGDYIYLATPQVENGACVSSFI
                     ISGTTAATRASDMVTIPTENNIYNRPLTCLVEVNRNWGDIPPNVAPRIFDFSGVPPIE
                     SITYAFNTTEKYYGQLYMQTYKASTSSYVSSLFTGRTDVRKLIGGFNIYSDGTKRVVS
                     NGEATKTMKTEWTGVKTRTFIRIGGQATSGTRHLFGHLRNLRLWHKELTDAQMGESIK
                     "
     gene            21840..22121
                     /locus_tag="SI621_002290"
     CDS             21840..22121
                     /locus_tag="SI621_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065003.1"
                     /translation="MKDLTLKFADRTDFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDSQISSLFDEYVVAVEHQLRGWM"
     gene            22131..23171
                     /locus_tag="SI621_002291"
     CDS             22131..23171
                     /locus_tag="SI621_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEI4065004.1"
                     /translation="MATSTVIPDDIKTLKSDVSKLKNDQGSYATKLYVDSKDEIVGDW
                     SASWYQQVLPTSGAIFGRKLRSTHRTAGVEDAYCELYLKKWIDSPGNAMARLNLNDNG
                     TNICWDFTNLYGGTMIFPGDSGYLKMGNCLMSYSKRGSNALIKFDYTDTLQIKYANHG
                     STMTLNTQGTAYAGVTAQLWGNSSRPVVYEVGVDGGAYMFYAQKNTDNTYMLSVNGAC
                     HATAFNQHSDRDLKDNIQVIDNATDRIRKMNGYTYTLKENGMPYAGVIAQEALEAIPE
                     VVGSAMKYQDGASGSEGEEGERYYTVDYSGVTGLLVQVARESDDRITALEEENAELRQ
                     RLSAIEAALVSK"
     gene            23214..23507
                     /locus_tag="SI621_002292"
     CDS             23214..23507
                     /locus_tag="SI621_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065005.1"
                     /translation="MDITPFLHALCAVAAQLLIGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            23527..23637
                     /locus_tag="SI621_002293"
                     /pseudo
     CDS             23527..23637
                     /locus_tag="SI621_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(23735..24325)
                     /locus_tag="SI621_002294"
     CDS             complement(23735..24325)
                     /locus_tag="SI621_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEI4065006.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         6140 a         5620 c         7210 g         5574 t
ORIGIN      
        1 cctcgttttt acggggtttt gttatattta aacattaccg aataggtata aatctcgatt
       61 gtcgatggta ttggttgtgt gacaatacct agtctttccg gtatgcctga agagaataca
      121 aacgacagat tatgtaaggg aaacgcgctg aatctttaac tctttcatgc tgagtgattt
      181 gacgttatat ttttagccgt aagtgaagag caaacgcatg gagcgacaaa atgcataaag
      241 atcaatacac tgatgccccc tcccaggagc aggttcgtgt aaaaactatg cttaatagca
      301 ccatttcgat gggttacccg gatgttgtaa ttgcatgtat agaacatcaa gtgtctctgg
      361 aagcattcag ggcaattgag gccgcgttag taaagcacga taagaatacg aaggattatt
      421 ccctggtggt tgactgatca ccataactgc taaccattct gaccatttca caagtgacag
      481 agtcaatatc gtattctgtc actgtcaggc taatacagca atgcaattca actacagcaa
      541 tgcctcgtat ttaggtgaat ttacaatatc gtccagttcg gatgccggct gcatttctga
      601 agataaggca tttcggttct cgtattttcc cctcatgctc gtcagtcctg cgcgtagtaa
      661 gaaacaggac actcacacta atttgtgtgg gcatgctgtg atgtccttct gaattatccc
      721 catgccatta tgtaaagctc tgttacagat gctcgtcacg gttgtcaggc tgtcgggtcc
      781 tcccagtggg gggccctgcc acggggcggg agcgtcgcgg aaaaaggcta gtttttgaaa
      841 tttcatccgt catcaccact actgtaatag attgatatta cagtattttt atttttgtgg
      901 tgtcgatttt gattgttttt tgttcatcac taacaccgtt tgcctaaagt cgttcgcaag
      961 atgcatgttt aaaacattct ggagcgggta tggatcgaga gttaaaaaat ctgacgctga
     1021 atatcagtca attggcggca ctgtcaggtg tacatcgcca gactgctgcg gcaaggctgc
     1081 aaaatctacc cgttgcaggg gggcatgaaa gcaacctcaa gctttatcgg gtggttgata
     1141 ttgtgtcggc atttctggca ttaccaccgc cggttgcaga aggcgaaatg gacgcacatg
     1201 agcgcaaagc ctggtatcag tctgaacgtg agcgtcttaa gttcgaacag gaaacggcac
     1261 aactcattcc ggccagtgat gtcagacggg agtttgccat ctgggcaaaa gcggtcgtgc
     1321 aggtgctgga gacattaccg gatattcttg aacgtgactg cggtctgcag cctgccgctg
     1381 tgagccgtgt tcagtccatt attgatgatc tgcgcgatca gatagccctg cgggtgactg
     1441 aagcaggtgc ggatgatgag gaggaattac agcaggagga gtaatgctga atcaggaaac
     1501 cgcaaaggca gcacgaaccg attcaggtta tatccttcgc gcaccgagac gaatgcgggt
     1561 tgctgatgcc gttgctcagt atatgcgggt gcccatgggg gcagggaact cagtcccgtg
     1621 ggatccgctg gtggcaccgt atgttattga gcctatgaac tgcctggcct cgcgtgaata
     1681 cgacgcagtg atatttgttg gcccggcacg aaccggcaag actatcggcc tgattgacgg
     1741 ctgggtgatt tacaacgtga tttgcgatcc tgctgatatg ctgatcattc agatgacgga
     1801 ggaaaaagcc cgcgaacact ccaaaaaacg actcgccaga acgtttcgcg tcagcccgga
     1861 agtggtcagt cgcctgagtc cgaacaaaaa tgacaacaac gtttatgaca gaacattcct
     1921 tgctggcaac tacctgaaaa tcggctggcc gtcagtcaat atcatgtctt catcagatta
     1981 taaatgcgtg gcgctgacgg attatgaccg ttttccggaa gatattgatg gtgaggggga
     2041 tgccttctct cttgcctcaa aacgtaccac cacatttatg tccagtggta tgacgctggt
     2101 ggagagttcc cccggcaggg atgtgaagga tgtgaaatgg cgacggactt caccgcatga
     2161 ggctccacca accacgggga tcctgtcgct ctataaccgt ggcgatcgcc gtcgctggta
     2221 ctggccctgt ccacactgtg gtgagtattt tcagccctgc ggcgatgtgg ttgctggttt
     2281 ccgtgatatt gccgatcctg tgctggcaag tgaggcggct tatattcagt gtccttcctg
     2341 ttcaggacgg attttgcctg aacaaaaacg cgagctgaac ggacgtgggg tctggttacg
     2401 ggatggtgaa tccatcaatg cagatggcag tcgttatggt gatccccggc gttcacgtat
     2461 tgcgtcattc tggatggagg gtccggcagc tgcttatcag acactctcgc aactcgttta
     2521 caaactgctt actgcagaac aggaatacga gacaaccgga agtgaagaga cactcaagac
     2581 ggttatcaat accgactggg gattacctta tcttccccgc gccagcatgg agcaacgaaa
     2641 aagtgaattg cttgagcagc gggcagagcc agttccttcc cgcagtgtgc cggatggcgt
     2701 taatttcctt gtggcgacag tggatgtgca ggcgggacgt catcgccgtt ttgtggttca
     2761 ggtaacgggc tatggcagcc gtggcgaacg ctggattatt gatcgttaca acatcacgca
     2821 gtcattgcgc ggtgacagcg acggggagag ccagcgaatt gatccggcca gctatccgga
     2881 agactgggat gtcctgctga cggatgtttt tcataaaagc tggccgctgg cctccgaccc
     2941 ttctcaacaa atgcgactga tggcaatggc ggtggactcc ggcggtgaag acggggtcac
     3001 tgataatgcc tataaattct ggcgtcgttg ccgtcgtgat ggccttggta aacgtattta
     3061 cctgtttaag ggcgacagca tccggcgagc aaaactgatc acccgtacat tccctgataa
     3121 caccggacga acgggccgac gggcgcaggc cgcaggtgat gtgccgctct ggcttcttca
     3181 gacggatgcc ctgaaagacc gggtgaataa cgcgttatgg cgtgactcgc taggtcccgg
     3241 ctatgtgcat ttccctgact ggctggggag ctggttttac gacgaactga cgtatgaaga
     3301 gcggagcagt gacgggaaat ggagtaagcc gggtcgcggt gccaacgaag cttttgacct
     3361 gatggtgtat gccgaggctc tggtcattct gcatggatac gaaaagatcc gctggccgga
     3421 tgcaccggag tgggcgagcc gggaaacctg gctggagtgt gtcccggaca gtaccgaacc
     3481 gtcaccctca ccggaaccgg tacccacgcc tgttaaaaaa caaaaacgga agaaaacagt
     3541 aactgacgat gttaacccct ggctgacttc cggaggatgg ttatgaatca gaatgatatt
     3601 gaagccatga ttcagcgtta tacggaagct gaaatggcgg tgctggacgg aaaatccgtc
     3661 acttttaatg gtcagcagat gaccatggaa aacttatctg agatccggca ggggcggcag
     3721 gagtgggagc gccgccttgc ggctctgatt acacgacgac gggggcatcc cgggtaccgg
     3781 ctggcgaggt tctgatggca attcttgatg atgtgattgg cgttttttca ccaggatgga
     3841 aagcggcaag gctgcgttcc cgtgcggtga tccaggctta tgaggccgta aaaacgacgc
     3901 ggacacacaa agcccgacgg gagaaccgaa ctgccgacca gttaagccag tacggggccg
     3961 tgtcgttacg tgagcaggct cgttaccttg ataacaacca cgatctggtc attggtgtat
     4021 ttgacaagct ggaagaacgg gtggtgggga aaaacgggat tattgtcgag ccacatccgg
     4081 tattacgcaa tggggccatt gcccgtgatc tggcagcgga gatacgtacc cgatggagtg
     4141 aatggtctgt cagtccggag gtcaccgggc agtttacccg tccgatgctg gaacgtctga
     4201 tgctgcgtac ctggctgcgc gatggtgagg tgtttgccca gatggtttcc gggcgcataa
     4261 acagcctgac gccttctgcc ggtgttcatt tctggctgga ggcgctcgag ccggacttta
     4321 ttcccatgac cagtgatgag agcaacaggc tgaatcaggg cgtgtttgtt gatgactggg
     4381 ggcgtcccga aaaatatctg gtgtataaaa gccgtcccgt atccgggcgg cagatggaaa
     4441 ccaaagaagt ggatgcagag cgaatgctgc atcttaaatt tgttcgccgt ctgcaccaga
     4501 tgcgcgggac gtctttgttg tccggtgtgc tgatccgcct cagtgctctg aaagagtatg
     4561 aagattctga gctgactgca gcaaggatcg ccgctgctct ggggatgtac atccggaaag
     4621 gcgacggaca gagctatgaa acggatggta atgacagcaa ggagaatgaa cgcgagctta
     4681 ccattcagcc aggcattatt tacgacgatc tgaaacccgg cgaagaaatc ggaatggtga
     4741 agtcggatcg ccccaatcct aaccttgaaa cttttcgtaa tggtcagttg cgtgccgtgg
     4801 ctgcgggcag tcgtctgagt ttttccagta cagcgcgcaa ctataacggc acttacagcg
     4861 cccagcgtca ggagctggtt gaatctactg atggctacct gatcctgcag gactggttta
     4921 ttggtgccgt cacccgcccg atgtaccgtg cctggctgaa acaggctgtg gtatccggtg
     4981 ttatcaggct accccgcgat ctggaccgat attcactgta taccgcggtg tattccggac
     5041 cggtgatgcc gtggattgac cctgttaagg aggctgaggc ctggaaaatt cagattcgtg
     5101 gtggagcggc gacagaatca gactgggtac gtgcaggtgg tcgtaatcca gatgatgtca
     5161 aacgtcggcg caaggccgaa attgatgaaa accgcaagct ggatctggta tttgataccg
     5221 atccggccag tgataaagga ggcagcagtg ccgcaacgaa acgacaggag ccgcagcaca
     5281 ccgacgacca gtccgaagaa taattcctgg ttcaggatgc aggcaggtca ccagagtgac
     5341 gcggatattt atatttatga cgagattggt ttctggggtg ttacggcgaa gcagtttatc
     5401 agtgatctga atgcactggg cgatatcacc cacattaatc tccatattaa ttcaccgggt
     5461 ggcgatgtct ttgaaggcat cgccattttt aatgcactga aaacacatgg tgcgtccatt
     5521 accgtttatg tcgacggtgt ggcggcgtca atggcgtcgg tcattgcgat ggtgggaaac
     5581 ccggtcatta tgccggaaaa ctccttcatg atgattcata aaccatttgg ctttacgggc
     5641 ggtgatgcgg aggacatgcg cacctatgcc gacctgctcg ataaagttga ggcggttctg
     5701 ttacccgctt atgcacagaa aaccgggaaa accaccgatg aaattgctgc catgctggcg
     5761 gatgagacct ggatgtccgg tgccgaatgt ctggcacagg gatttgctga tcaggtgaca
     5821 ccagccgtta aggcaatggc atgtattcag tcaaaacgta cagaggaatt taaaaagatg
     5881 ccggaatcca ttcgaaatat gattactccg ccacgcaaca gtgctccacg cgtacaggat
     5941 gatggacctg cagcctcccg gacgccagtg caggcagcag cacccgtggt ggatgaaaac
     6001 agcatccgtg cgcaggtcct ggcagagcaa aaagcgcgtg taaacggtat taatgatctg
     6061 tttgccatgt ttggcgggcg ttatcagtcg ttgcaggctc agtgtcttgc cgatcctgaa
     6121 tgttcgctgg agcaggcccg cgaaaagctg ttgaacgaga tggggcgcga gtccacgcca
     6181 tccaataaaa ataccccggc tcatatttat gccggtaacg gtaattttgt gggggacggg
     6241 atccgccagg cgctgatggc gcgcgccggg tttgaaaaaa ctgaacgtga taatgtctac
     6301 aacgggatga ccctgcgtga atatgcccgt atgtcactga ctgaacgtgg tattggtgtt
     6361 tccagttata acccgatgca gatggtcggt gcggcgttca cacacagtac gtctgacttc
     6421 ggtaatattc tgctggatgt tgcgaacaaa gccattctgc agggctggga agatgcccct
     6481 gaaacctatg aacagtggac gcggaaaggt cagttgtctg attttaaaat tgcccatcgt
     6541 gtgggtatgg ggggcttcag tgctctgcgt caggtgcgtg aaggggcaga atataaatac
     6601 gtcaccaccg gagataaaca ggccactatt gcactggcga cctatggcga gctgttcagt
     6661 atcacccgtc aggccattat caatgatgat ctgaatatgc tgaccgatat cccgatgaag
     6721 ctaggccgtg cggcgaaatc cactattgcc gatctggttt atgccattct gacgtctaac
     6781 ccgaaaatct ccacagataa tgtaagtctg ttcgataaag cgaaacatgc aaacgtactg
     6841 gagagcgcgg caatggacgt ggcatcgctg gataaagccc gccagttgat gcgcgttcag
     6901 aaagagggtg agcgtcatct gaatattcgt cctgcgttcg tactggtacc gacggcgatg
     6961 gagtctgttg ctaaccaggt cattcgctcc tcaagtgtca agggggctga cattaacgcc
     7021 ggtattatta acccggtgaa agattttgcg accgttattg cagagcctcg tcttgatgat
     7081 aacagccaga ccaccttcta cctggctgcg tccaaaggct ccgatacgat tgaagtggct
     7141 tatctcaacg gtgtggatac gccatatatt gatcagatgg agggcttcag tgtggatggc
     7201 gtgacaacga aagtgcgtat tgacgctggt gttgcgccag ttgatcaccg cggcctggtg
     7261 aaatgtacgg cgtaaacgtc gcagacaaca actctgatgg cccgtaaggg ctttttttgt
     7321 acctgaaatc agcccctgaa cggggctgtg cggagacagt tatggcaaag aattttgtag
     7381 aagaaggaaa aacggtggcg attgttgcca gtgcagccat cagcagcgga gatctggtgc
     7441 gggtgggtga tgtttttgcg gtggcgctga ccgatattcc acagggagaa acaggcgacg
     7501 gcataaccga aggtgtgttt atgctgccta agctgaaaac ggatgacatg aaaacgggta
     7561 agaaggttta tctgaagtca ggaaaagttc agctgactaa cagcggctct gatccgctgg
     7621 tcggggttgt atgggcagat gccggaacca gtgcagaaga agtgccggta aaactcaatg
     7681 cctgatccct tttcccggct ggcagcgcgt atggatgcga tcacggtcag aaagatggga
     7741 aagacagcct cgattaatga tgtcgatatg actgtgatcc cgggagaaac actggcagag
     7801 ctgaatgctc tgtccggacc tgcggtctct ctggtggtgt tttcttcggg ataccgccca
     7861 cggcgcgggg atcgcgttgt ttatgacgga caacaatgga cggtcacacg gcatgaacgt
     7921 tttaacggta agccaatgat ctttattgag taaagaggtg tgggatgaag gggcttgaga
     7981 atgccatccg caatctgaac agccttgata cccgtatggt gccacaggcc agcgcatggg
     8041 cgataaaccg tgtggcacag aaagcggtct cggttgccac ccggcaggtt gccgggaata
     8101 ccgttgcggg agataaccag gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc
     8161 gggtgtttaa agccagtccg tcaggaaaaa tgacggccag gatccgcgtt aaccggggca
     8221 atctgcccgc cattaagcta gggacagccc gggtcagact ggcccggcgt ggtggaaaac
     8281 tgcagtaccg tggcagtgtg ctgaaggtgg gtaaatatct tttccgggat gcgtttattc
     8341 agcaactggc gaatggtcgc tggcatgtga tgcgacgtat tgatggcaaa aatcgttacc
     8401 ccattgatgt ggtgaaaatc ccgctgtccg gaccgctgac acaggcattt gaagatgccc
     8461 gcgaccacat cattgctgcg gaaatgccga aacagctggg gtatgcactg aaacaacaac
     8521 tgaggttatg gctgacccga tgaaccgaca tacacaaatc cgccaggccg tactggcacg
     8581 ccttcgggaa cagtgtggag acagcgccac gttttttgac gggcttccgg catttattga
     8641 tgcgcaggaa ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat
     8701 gacggatgaa gatgactggc aggctgttct gcatattgcc gtcttcatcc gggcacaggc
     8761 actggattca gagctggata tgtggatgga gagcaccatt ttccctgccc tgaatgatat
     8821 accggcactt tccggactca tcgacaccct gatcccactc ggttttaact atcaacgtga
     8881 taatgagatg gccacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta
     8941 aggaggtggt aatgaccaca ccaaatccac tggcaaagac gaaaggtgcg gggacgacgt
     9001 tctggatgta taccggcaac ggcgatgcgt ttgcgaaccc tttgtcggac actgactggc
     9061 tgcgtcttgc gatggtgaag gatctgcaac ctggcgaaat gaccgctgat gcagaagatg
     9121 acacttatct cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg
     9181 ttggtgatac ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg
     9241 ttcagttgtt cgactccggt gaagtctgcg cgtttcgtat caaatatccc aacggcactg
     9301 ttgatgtttt ccgtggctgg ctgagctcac tgggtaaaac cattgcctca aaagacgtga
     9361 tgacccgcac tgtgaaaatc agcggtgtgg ggcgtccgta tctggcagag gaaggcactg
     9421 aaaccgtgag cgttaccggg ctgacagtat caccggcgtc tgccagtgta aaagtgggag
     9481 caaccaccac gctgaccttt acagtaaaac ctgacggagc cagtgacaaa gcgatcagtg
     9541 tgcattcgtc agatccacag actgccacgg ttaccctgaa cggacttgtg gccacggtaa
     9601 aaggcgtgaa gcagggcagt gtcagcattg tgggcatgac cgctgacggg aattttgtgg
     9661 ctgtgactgc ggtgactgtc agcgcagcag gttaacagga cgatactcat catttgcccc
     9721 ggttatgcgg ggcttttttg caggtggaga acatgatgtt tctgaaacag gacacgttta
     9781 attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga attgaatatc
     9841 tgacgtttgt tcagcagcga acggcaaagt ttgatgccgg ggagggagaa ctgccggagg
     9901 ctgaacgaca gattgctttt ctgcggatgg ggatggatat caatgcctgg ctggtttccc
     9961 gctcactgtg gaatgcggat cagtctaagg atgtagagac gctttgcgca tccgttatta
    10021 caacatggtc gtatgatgcc ctgggtgcgg gggcggagat ggttctgtcg ctgagcggta
    10081 tgggggccat tgataatgct gtggatgatg agcatgaggc gctgacgccg gaaaagtcct
    10141 gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg cagactggcg
    10201 gcggatgctg tcggaaatgt cggccactga gcttggtgag tggggcgatt atttccggat
    10261 gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt
    10321 gagaatggtg tccggcagca gtgatgctgc ggtggctgat ttcagccttt taccggaaga
    10381 gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag gtatttccgg
    10441 aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt gatgcggcag
    10501 agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag
    10561 aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcc gcccggcaga
    10621 caacgcaggc agcttcttcg gctgcaacag ccgcatccgt ccatgcgcag acggtggaga
    10681 agaacgcaca ggctcatgaa cgcatggccc gcgaggtgga gaaaacccgc cagcgcatgg
    10741 aggcactgag ccagaaaatg cgcgaggaac aggcgcaggc catggctctg gcggaggctc
    10801 aggataaagc ggctgctgcg ttttatcgtc agattgacag tgtgaaacag gccagtgcgg
    10861 ggctgcagga attacagcgt attcagcagc agatccgaca ggccagaaac agtggcggga
    10921 ttggtcagca ggattatctg gcgctgattt ctgaggttac tgcgaaaacc cgtgttctta
    10981 cacaggctga ggaagaggct acccgacaga aagtggcgtt tatccgtcag cttaaagagc
    11041 aggcaacccg cctgaatctt tcatcttctg agttgcttcg tgctaaggca gcccagctgg
    11101 gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcaggcagga aaagccacgc
    11161 attcgctggg tctgaaaagt gcagcagccc gccaggagat aggcgttctg ataggtgaac
    11221 tggcccgcgg caatttaggg gcgctgaggg gatccgggat aacgctggct aaccgtgccg
    11281 gatggataga cacactaatg tcacagaaag gcatgatgct tggcggggtt attggcggta
    11341 ttgccgcggc cgtctatggt ctgggtaaag cctggtatga cggtcagaag gagggggaag
    11401 aatttaaccg ccagttgtcg ctgacggggc attatgccgg agtcactgcc gggcagctgt
    11461 ggacgctcag tcgtgctatt tccgggaatg gtatcacgca acatgctgca gccggtgcgc
    11521 tggctcaggt ggtggggagt ggtgcatttc gtggaaacga tatcggtatg gtggcgagag
    11581 ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta
    11641 agcggctgaa ggatgatcct gtaaatgccg cgaaggctct ggacaatgag ctgcattttc
    11701 ttactgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg
    11761 ctgcacggat agccatgtct gcactggcag aggaaaccgg tcggcgtact gcggatattg
    11821 ataataacct caatgcgctt ggcagtacgc tgaagtatct gtctgatttg tggagtcgtt
    11881 tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgaacag attgccgctt
    11941 tacaggagaa agtgtcgcgg gcgaaaagac tcccctggac ggcatcatct tctcaggttg
    12001 aatacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccagaag gatttgcagg
    12061 atgcaaaaga gcaggcagag cggaattatc aggagcaaca gaaacgccgt aatgctgaaa
    12121 atgctgcact gaaccggatg aatgaaacgg aagcagcacg acatcagcgt gaaattgcgc
    12181 gtattaatgc catgcagtac gccgatcagg ctgtcaggga tgcggcgata caacgtgaaa
    12241 atgaacgtta cgagaaagcc ctggcatccg gtaagaaaaa aacacgtgaa acccgtaatg
    12301 atgaggccac ccggttattg ctgcagtaca gtcagcaaca ggcacaggtg gaaggacaga
    12361 ttgctgctgc cagacagtca gcaggcattg ccacggaaag gatgacagaa gcgcataaac
    12421 agcttctggc tctgcagcag cgcatcagcg acctggacgg gaaaaaactg acggcagatg
    12481 aaaagagtgt gctggcccgt aaagatgaac tgattcaggc actgacgctg ctggatgtaa
    12541 aacagcagga gcttcagaaa cagacggcac tcaacgagct gaagaaaaaa acaattcagc
    12601 tgaccagtca actggctgaa gaagagcgcg ctcagcgtca gcaacatgac ctggatatcg
    12661 ccacggtggg tatgggtgat cagcagcgac agcgatatca ggtacaactg agtcttcgcc
    12721 agaaatacca gcaacagctg gagcagttga ggcgggatag tgagcagaaa gggacatata
    12781 acacggatga ctacagaaag gccgagcagg cgctgacgga gagcctgaac cgacaactga
    12841 atgagaatcg ccgttactgg caacagcttg aagttgtgca gggtaactgg aaaaacggag
    12901 tcctgcgtgc atttcaggat tttaccgtgg atgcagataa tacggcagaa acagcagaac
    12961 aggtgttctc gtcagccttc agcaacatgg gaaatggcct ggcaactttt gtcactaccg
    13021 gcaaactcaa tttcaaatcc ttcacctctt ctgtgctgtc agatatggcg aaaatcctgg
    13081 cgcaggcaac catgatgaaa tcgataaaag ggattggcag tgtactggga tttgatctca
    13141 gcagcctttc cctgaatgcc aatgggggga tttatcagtc tgctgatttg agtcgttaca
    13201 gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc aaaaggcgcg ggtgtgatgg
    13261 gggaagcggg acctgaagcc attctgccat tgcgtcgtgg tgctgacggt aagctggggg
    13321 ttgtggcgga tattgggggt tcaggtatgg cgatgttttc cccgcagtac aacatcgaga
    13381 tcaataacga tggcacgaac gggcagatag gtccggctgc cctgaaggcg gtttatgacc
    13441 tcgggaaaaa agcggcagcg gactttatgc aacagcaggc ccgtgatggt ggtcggttaa
    13501 gtggagcata tcggtaatgg agacgtttca ctggaaagtg cgcccggata tgaatgtggt
    13561 atcagagccg aaagtggtga cagtgaagct gggcgatggt tatgaacagc gtcgtgcggc
    13621 gggactgaat aaccagttgt cgacttacag cgtgacgata cgtgttcgta aatgtgaaca
    13681 cccatcttta aaagcctttc tggaacggca cggtggcgtc cgtgcatttc agtggacgcc
    13741 accttatgac tggaaaccga tcagggtggt ttgtcgtaaa tggtcggcaa gcgtgggggc
    13801 gctgtgggta accataacgg cagattttga acaggtcgtg gcataggagg ctctgatgca
    13861 ggatattcca caggaaacac atcatgagac gacacgcctc actcagtcag cccaggtggt
    13921 gctctgggaa atcgatctga cagaggtcgg tggtgaacgt tattttttct gtaatgagca
    13981 gaacgaaaaa ggtgagccgg ttatctggca ggggcggcag tatcaggtat accccattca
    14041 ggggacggga tttgaactga acggcaaggg cagtgctgcc cgtccgacac tgacggtttc
    14101 taacctgcac ggcatggtca ccgggatggc ggaagacctg cagagtctgg tcggaggaac
    14161 ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa
    14221 cagtgacgcc gatccggagc aggaggtgat cagccgctgg cgcatcgagc agtgcagcga
    14281 actgagcgcg gtgagtgcct cttttgtact gtccacgccg acggaaacgg acggtgccgt
    14341 ttttccggga cgtatcatgc tggccaacac ctgcacctgg acctatcgcg gtgatgagtg
    14401 cggttatcac ggtccggcgg tcgcggatga atatgatcag ccgacgtccg atatcacgaa
    14461 ggataaatgc agcaaatgcc tgagcggctg taagtttcgc aataacgtcg gcaactttgg
    14521 cggcttcctt tccattaaca aactttcgca gtgaatccca tgacagagac agaatcagcg
    14581 attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt ggtaagcacg
    14641 ccggaggggg aaagatattt cccctgcgtg aatatctccg gtgagccgga ggcgtatttc
    14701 cgtatgtcgc cggaagactg gctgcaggca gaaatgcagg gtgagattgt ggcgctggtc
    14761 cacagccacc ccggtggtct gccctggctg agtgaggccg accggcggct gcaggtgcag
    14821 agtgatttgc cgtggtggct ggtctgccgg gggacgattc ataagttccg ctgtgtgccg
    14881 catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
    14941 gcttatcatc tggcggggat tgagatgccg gattttcatc gcgaggatga ctggtggcgt
    15001 cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg
    15061 tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat
    15121 gccgccattt actgtggtga tggcgagctg ctgcaccata ttcctgaaca actgagcaaa
    15181 cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca
    15241 tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gatcttcgtg
    15301 tgaaaacggg ggctgaagct atccgggcgc tggccacaca gctcccggcg tttcgtcaga
    15361 aactgagcga cggctggtac caggtacgca ttgccgggcg tgatgcaggt gaaaccgaat
    15421 tgtctgcccg tcttaatgag ccgctggcaa atggtgccgt gatccacatc gtgccgcgtc
    15481 tggcgggagc taaaagtggc ggtgtgtttc aggtggtgct gggggcggcg ctgattgcgg
    15541 tggcatggtg gaaccctgtg ggctggctgg gtgccgcggc tgtatcgggc atgtatgcgg
    15601 caggggccag tatgatcctg ggcggagtgg cgcagatgct ggcaccgaaa gccaggacgc
    15661 ccacggcagc aagtacagat aacggcaaac agaacaccta tttctcctca ctggataaca
    15721 tggttgccca gggcaatgtt ctgcccgttc tgtacggtga aatgcgcgtg gggtcgcggg
    15781 tggtctctca ggagatcagc acggcagacg aaggggatgg tggtcaggtt gtggtgattg
    15841 gtcgctgatg aaaaacgttt tatgtgaaac cgcctccggg cggttttgtc gtttatggag
    15901 cgttaggaat gggtaaaggc agcagtaagg ggcatactcc gcgcgaagcg aaggacaacc
    15961 tgaagtccac gcagttgctg agtgtgatcg atgccatcag cgaagggccg attgaaggtc
    16021 cggtggatgg attaaaaagc gtgctgctga acagtacgcc agtgctggac actgagggga
    16081 ataccaacat ctccggcgtc acggtggtgt tccgggccgg tgagcaggag caaacaccgc
    16141 cggagggatt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg aaatacgaca
    16201 cgccgatcac ccggaccatc acgtcggcaa acattgaccg actgcgtttt accttcggcg
    16261 tgcaggcact ggtggaaacc acctcaaagg gggacaggaa tccgtcggaa gtccgcctgc
    16321 tggttcagat acaacgtaac ggtggctggg tgacggaaaa agacatcacc attaaaggca
    16381 aaaccacctc acagtatctg gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta
    16441 atatccggat gcgcaggatg acgccggaca gcaccacaga ccagctgcag aacaaaacgc
    16501 tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcactgg
    16561 ttggcgtgca ggtggactcg gagcagttcg gcagccagca ggtgagccgt aattatcatc
    16621 ttcgcgggcg cattctgcag gtgccgtcga actataaccc gcagacgcgg caatacagcg
    16681 gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg tgtctgtggg
    16741 atatgctgac ccatccgcgc tacggcatgg ggaaacgtct tggtgcggcg gatgtggata
    16801 aatgggcgct gtatgtcatc ggccagaatt gcgaccagtc ggtgccggac ggctttggcg
    16861 gcacggagcc gcgcatcacc tgtaacgctt acctgaccac acagcgcaag gcgtgggatg
    16921 tgctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg cagacgctga
    16981 cgttcgtgca ggaccgacag tcggataagg tgtggaccta taaccgcagt aatgtggtga
    17041 tgccggatga tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac cgccataatg
    17101 ccgttgaggt gaactggatt gacccgaaca acggctggga gacggcgaca gagcttgttg
    17161 aagatacgca ggccattgcc cgttacggtc gtaatgtcac gaagatggat gcctttggtt
    17221 gtaccagtcg ggggcaggca caccgagccg ggctgtggct gattaaaacg gaactgctgg
    17281 agacgcagac cgtggatttc agcgtgggtg ctgaagggct tcgccatgta ccgggcgatg
    17341 tcattgaaat ctgcgatgat gactatgccg gtatcagcac cggtggtcgc gtgctggcgg
    17401 tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgcca tcctccggta
    17461 ccacgctgat aagcctggtt gacggaagtg gtaatccggt cagcgtggag gttcagtccg
    17521 tcaccgacgg acttaaggtg aaagtgaatc gggttcctga cggcgttgca gaatacagtg
    17581 tgtgggggct gaagttgccg acgttgcgtc agcgcctgtt ccgctgtgtg agtatccgtg
    17641 agaacgatga cggcacgtat gccatcactg ccgtgcagca tgtaccggaa aaagaagcca
    17701 tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat ggtgtcacgc
    17761 cgccagcggt gcagcacctg accgccgaag tcaccgcaga cagcggggaa taccaggtgc
    17821 tggcgcgctg ggatacgccg aaggtggtga agggcgtgag ctttatgctt cgcctgaccg
    17881 tggcagcgga tgacggcagt gagcggctgg tcagcacggc ccggacgacg gaaaccacat
    17941 accgcttcac gcaactggcg ctgggaaact acaggctgac agtccgggcg gcaaatgcct
    18001 gggggcagca gggcgatccg gcatcggtat cgttccggat tgccgcaccg gcagcaccgt
    18061 cgcggattga gctgacgccg ggctattttc agataaccgc cacgccgtat cttgccgttt
    18121 atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattacc gatatcaggc
    18181 aggttgaaac cacagcccgc tatcttggta cggcgctgta ctggatagcc gccagtatca
    18241 atatcaaacc gggccatgat tattattttt acatccgcag tgtgaacacc gttggcaaat
    18301 cggcatttgt ggaggctgtt ggtcagccga gtgatgacgc atccggctat ctggattttt
    18361 tcaaaggcga gatagggaaa acccatctgg ctcaggagtt gtggacgcag attgataacg
    18421 gtcagcttgc gcctgacctg gctgaaatca ggatgtccat tacggatgtc aacaatgaaa
    18481 tcacgcagac cgtcaataag aagctggaag accagagtgc agcgatccag cagatacaga
    18541 aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcagcaga
    18601 tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaat acccctgacg
    18661 gtatgcagag tcaggtgctg ctggcggcgg acaggattgc gatgattaat cctgcgaatg
    18721 gcaatacaaa gccgatgttt gttggtcagg gcgatcagat atttatgaat gaagtgttcc
    18781 tgaaatatct gacggctccc accattacca gcggtggcaa tcctccggta ttttccctga
    18841 caccggacgg gcggctgacg gcgaaaaatg ccgatatcag cggtaacgtg aatgcgaact
    18901 ccgggacgct caacaacgtc acgattaacg agaactgtcg ggttctggga aaactgtccg
    18961 cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa agctttcccc cgggactccc
    19021 gtgcaccgga gcggtggcca tcagggacca ttaccgtcag ggtttatgac gatcagccgt
    19081 ttgaccggca aattgttatt ccagcggtgg cttttagcgg tgccagacat gaacgggaga
    19141 atagcgatac ttattcgtca tgccgcctga tagtgaagaa aaatggggct gaaatttata
    19201 acagaacggc tctggataat actctgattt acacgggtgt tattgatatg ccagcaggca
    19261 gtggcgtaat gacactggag ttttctgtat cagcatggtg ggtaaatggc tggtatccca
    19321 cagcaagtat cagcgatttg ctggttgttg tgatgaagaa agccactgca ggcatcacga
    19381 ttagctgaat tttaaaaccc aaattcgggc accagaaatg gtgccttttt tatttgtgga
    19441 gtgaatatgg cagtacagat ttcaggtgtg ctgaaagacg gtgcaggaaa accaatacag
    19501 aactgcacta ttcagctcaa agcaaagcgt aacagcacca cggtactggt gaacacagtg
    19561 gcctctgaaa atccggatga agccgggcgt tacagcatgg atgttgagta tggccagtac
    19621 agcgtcatcc tgctggttga aggttttccg ccttcacatg ccgggaccat taccgtctat
    19681 gaaggctcca gaccaggtac gctgaatgat tttctcggtg ccatgacgga ggatgatgtc
    19741 atgccggagg cattgcgtcg ttttgaggaa atggtggaag aagcggcacg caacgctgaa
    19801 gccgcctctc agagcgcagc ggcggcaaag aaatccgaaa cggctgcggc atcatcgaag
    19861 aacgcggcga aaacctcaga aacgaatgca gctaacagcg cacaggcggc agcggcctcg
    19921 cagactgcat cggcaaactc cgcgacagca gccaaaaaat cagaaaccaa cgcgaaaaat
    19981 agcgagacag ccgcaaagac gagcgaaacc aacgcaaagt ccagccagac ggcagcgaaa
    20041 accagcgaaa cgaatgccaa agccagtgaa actgcggcga aaaatagcca gaatgcagca
    20101 gccgaaagcg agagtgcggc agctggttct gcgacttcag cagctggatc agcaactgct
    20161 gcggctaaca gccagaaagc agcgaagacg agcgaaacta acgcaaagtc cagccagacg
    20221 gcagcgaaga ccagcgaaac gaatgccaaa gccagcgaaa ctgcggcgaa aagcagtcag
    20281 gatgcagcag ctgaaagcga gagtgccgca gctggttctg caagtgcggc ggctgcttct
    20341 gccactgcat cagcaaacag tcaaaaagct gcaaaaacca gtgaaaccaa tgcaaagaca
    20401 agcgagactg cagcggcgaa ctcggcgcaa gcatcggcag caagccagac agcagctaaa
    20461 gcaagtgagg atgcagccag agagtatgca agccaggctg cggagccgta taaacaagta
    20521 ttgcagccgc ttcctgatgt gtggatacca tttaacgatt cgctggatat gattacgggt
    20581 ttttcgccat catataaaaa gattgttatt ggtgatgatg aaataacgat gcctggcgac
    20641 aaggttgtta agtttaaacg cgcatcgaaa gcaacctata ttaataaatc tggtgtgctg
    20701 acagaggctg ccattgacga gccacggttt gaacgtgatg gcctgcttat tgaggggcaa
    20761 agaacgaact acatgctcaa ttcggaaagc cctgccagtt gggggcgatc gtcaaatatg
    20821 gatgttcccg aaaccgggac ggatagtttt ggttttacct atggaaagtt tgtctgtaac
    20881 gattctctga ttgggcaaac ctcagccatt aatatggcat caattgctgc aacaaagtca
    20941 gttgatgtct caggcgataa taaatacgtg acaacctcat gtcgttttaa aacagaactg
    21001 caggtaaggt tgcgtatccg atttgataaa tatgacggta gcgcaacaac ttttcttggt
    21061 gatgcgtata ttgatacaca aacgcttgaa attaatatga caggtggtgc ttccggtaga
    21121 attacggcac gagtcaggaa ggatgaaact acaggatgga tttttgctga ggcaacaatt
    21181 caggcaattg atggtgagtt aaaaataggc tctcagatac agtattcacc taagcaggga
    21241 ggggcaaccg tatcaggtga ctatatttat ctggctaccc cacaagtaga gaatggggct
    21301 tgtgtatcat cttttattat atcaggaacg acggcggcga ctcgtgcgag tgatatggtt
    21361 acgatcccga ccgaaaacaa catttataac agaccgctta cttgtttggt cgaggttaac
    21421 aggaattggg gcgatattcc tcctaatgta gcaccgcgta tttttgattt ttctggtgtg
    21481 ccgcctattg agtcaatcac atacgctttt aacacaaccg agaaatatta cggtcagctt
    21541 tatatgcaaa cttataaagc gtcgacaagt agttacgttt ctagtttgtt tactggtcga
    21601 acggatgttc gaaaactcat tggtggtttt aatatttatt ctgatggtac taaacgagta
    21661 gtttctaacg gtgaggctac taaaaccatg aaaacggaat ggacgggcgt aaaaacgcgg
    21721 acctttattc gaataggagg tcaagccaca tcagggacac gtcatctatt cggccatttg
    21781 agaaatcttc gtctctggca taaagaatta actgatgcgc aaatggggga gagtattaaa
    21841 tgaaagattt aacactcaaa tttgccgaca ggaccgactt ttcggccttt atggagagta
    21901 ttggctatta tgatgacgag tcgatgcagg atgatattct tattgacgtg ataggtaacg
    21961 tgtacaaaga aaccggagaa ctgactgaag atggcgaacc ggtatgtgtt aaggaagacg
    22021 gatattttgt aaacgtgcgc atcattaatg attcgcaaat atcgtcatta ttcgatgaat
    22081 acgtggttgc tgttgagcat caacttcgtg gctggatgtg aggaagaaaa atggctacat
    22141 cgacagtaat tcctgatgac atcaaaacgc taaaatccga cgttagcaaa ttaaaaaacg
    22201 atcaaggaag ctacgcaaca aaattatatg tagacagcaa agatgaaatc gttggtgact
    22261 ggtctgcttc atggtatcag caggtattgc caactagcgg agctatattt gggagaaaac
    22321 tccgctcaac tcacaggacg gcaggtgttg aggatgcgta ttgcgaacta tacctcaaaa
    22381 aatggataga cagtccaggt aacgcaatgg cgcgccttaa cctgaacgat aacgggacaa
    22441 acatttgctg ggactttacc aacctttatg gcggtacgat gatttttccc ggtgacagcg
    22501 gatacctcaa aatgggtaac tgccttatgt catacagcaa gcgtggaagt aacgcgctta
    22561 ttaaatttga ttacaccgac acattacaga tcaaatatgc caatcatggg tcaaccatga
    22621 cattaaacac acagggaacc gcttatgctg gtgttactgc tcaattgtgg ggcaactcca
    22681 gccgtcctgt tgtttatgaa gtcggtgttg atggtggcgc ttatatgttc tatgcgcaga
    22741 aaaataccga taacacctat atgttaagcg ttaatggtgc atgtcatgcc accgcattta
    22801 accagcattc cgaccgggat ctgaaagaca acattcaggt gatcgataat gcaaccgacc
    22861 gcatccgtaa aatgaacggc tatacataca cgcttaaaga aaacggtatg ccctatgctg
    22921 gtgtcattgc acaggaagct ctggaagcaa tcccagaagt tgtaggttcc gcaatgaaat
    22981 atcaggacgg tgcgagcgga tcggaaggtg aagaaggtga acgttattac acagtagatt
    23041 attctggtgt tactggcttg cttgttcagg tagccagaga gtcagacgac agaataacag
    23101 cactggaaga agaaaacgca gaattaagac aaagattatc tgcaattgag gcggcgcttg
    23161 tgtctaaata atattaaggg gccgagcgcc ccgttttatt gggtaggatg aaaatggata
    23221 tcacaccttt ccttcatgcg ctttgtgctg tggctgcgca gctactgatt ggtcttttta
    23281 ccgggaactg ggcttacggg gcgatagccg gttgtacgtt cttcattgcg cgtgaacaca
    23341 cccaggcaga atatcgctgg attgaaatgt tcgggcatgg caagcgaatg aatatgccgt
    23401 ggtggggcgg ttttgatccg cgcgcgtggg atgtggcaag cctgatggat tttgctgtgc
    23461 cggtggtggc gtgtctgctg gtctggctgt tggttaatcg tgggtgaaaa aaggtgagct
    23521 gtatatgcaa cggaggaaga aacctcgttg cgggaagcct ggaagaagta tcgggtgttg
    23581 ctgaaccgtg ttgatacgtc aactgcacag gatattgaat ggccagcact gccgtagggt
    23641 aaaacatata aattctataa ttagatgtat ctttccattt acggcaagga agggggcttg
    23701 gaagacgtaa ggcatctcac accgagatta ttttttatat gtcaggtgtc tgaagttttg
    23761 ctttggctct taaaatggtt tgccgcgagg ttttgaattc ccgggcaatg gcacttatac
    23821 ttacacctga cttaatccgt tcgaatacca cctgtttctg ttcttcattt aacacaggtg
    23881 gtcgaccaaa acgtttccct gcgccccggg ctcttactat cccggaatga gtgcgttcaa
    23941 gtaaaaggtc tcgttcaaat tcagcgactg ctgaaattac ttgcatcatc atttttcctg
    24001 ttggactggt caggtcaatg ccccccaatg ctaagcaatg cactctgata cctgtttcgg
    24061 tcagttgttc cactgttttc ctgatatcca ttgcattaca accaaggcga tccagttttg
    24121 tcacaatcaa ttgatcacca catttcaggc gagcaagcaa ccggttaaaa ccaggacgct
    24181 cactggttgc tgctgagccg ctaatgtgtt cttcgattat ttgctgaggt ttgattttaa
    24241 aacctgcact ttcgatttcc cggcgttgat tttcggtggt ctgatccagc gttgatatcc
    24301 gacagtaagc aaaaattcga gacatagtga gactctatac gaaattggtg ttcatatcat
    24361 aatgcatctc agaaaataat tttgattatt tttgtgcatg tttgtatgta cacgttcgaa
    24421 aataaacgaa tgcgtatgca accccgtaat tttggtgaga cccaaaatcg attttgtgaa
    24481 aaatggcttt aactcggttt gtttttcgag ttccgggcgg actcaaggaa gaagaatagt
    24541 gttg
//
LOCUS       DARNVM010000064        24103 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.064, whole genome shotgun sequence.
ACCESSION   DARNVM010000064 DARNVM010000000
VERSION     DARNVM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.064"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            361..894
                     /locus_tag="SI621_002295"
     CDS             361..894
                     /locus_tag="SI621_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEI4065007.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMM
                     KAVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIW
                     ISGNTIEYKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQ
                     AGGWQQVEGRARVEARA"
     gene            1022..1564
                     /locus_tag="SI621_002296"
     CDS             1022..1564
                     /locus_tag="SI621_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065008.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRK
                     YSIIIRLERERFFIVGSVDSLVWHGNPSVCTVSLTRNPVGIANDISRKILANALDEIE
                     LLREHESALQEKQDKVRIIKGMLSQLLSISPWYGALTGFNADNGLAGKILQNGDDYEM
                     VIRGLNVDQLIKVAGFIKKL"
     gene            1588..1857
                     /locus_tag="SI621_002297"
     CDS             1588..1857
                     /locus_tag="SI621_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065009.1"
                     /translation="MGLFMKTDNVFFKSKGSYSYVVTVDMDNQMYMVRKYVKTYVKSC
                     DKEQVKGKKTVKKLVLDTNLTLPELLGLDNGILADLFTRGNNNAN"
     gene            1847..2092
                     /locus_tag="SI621_002298"
     CDS             1847..2092
                     /locus_tag="SI621_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065010.1"
                     /translation="MQTKPTPADVKEARISAGLTLKEAAEIFGYQLNTWQMKESAGKA
                     TRSLSVGEYNYLLLLAGKHPEYVLCKRDTITAEPTTV"
     gene            complement(2123..2422)
                     /locus_tag="SI621_002299"
     CDS             complement(2123..2422)
                     /locus_tag="SI621_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEI4065011.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQK
                     QVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGKRVAFNI"
     gene            complement(2419..2769)
                     /locus_tag="SI621_002300"
     CDS             complement(2419..2769)
                     /locus_tag="SI621_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4065012.1"
                     /translation="MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHA
                     DTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAIILCAGDKSNDKKFYDRMIRIAD
                     EEFSTHLAELEGKK"
     gene            3135..3782
                     /locus_tag="SI621_002301"
     CDS             3135..3782
                     /locus_tag="SI621_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEI4065013.1"
                     /translation="MTVAIALVNGKGGVKKSSMSRAIATEYTKADWKVLGLDMDVDQG
                     SFLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVIDSAAYATKSLLSLAE
                     IVDLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVSESESEYRTALEYLK
                     PCGVQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQGVIDLLTKLSQ"
     gene            3822..4055
                     /locus_tag="SI621_002302"
     CDS             3822..4055
                     /locus_tag="SI621_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065014.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVD
                     MEVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
     gene            complement(4087..4350)
                     /locus_tag="SI621_002303"
     CDS             complement(4087..4350)
                     /locus_tag="SI621_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HEI4065015.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPDNDLAKITKSLRTAENRMVCVIQ
                     DNNRILRWDRSYASRAKNHWRKVAPDRFEILGTVEHIHYVGKC"
     gene            complement(4319..4834)
                     /locus_tag="SI621_002304"
     CDS             complement(4319..4834)
                     /locus_tag="SI621_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065016.1"
                     /translation="MQTITTREQLLVNGKVLERVATHIVTGAHGYETLCTGGYNLQYN
                     QERVLVQNCEKLAEGELPITCHTCFAVWQDAYKFKDSDFDIKSGNFTDTGLTDITIGQ
                     ISEPEEESVNILYADQYETLPIERYMTNEEVGRLVFNFMKRTNGEALLIVFPEYDNGD
                     DNDDKNTRCVK"
     gene            complement(4844..5308)
                     /locus_tag="SI621_002305"
     CDS             complement(4844..5308)
                     /locus_tag="SI621_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEI4065017.1"
                     /translation="MKILIIFALYILSPSLYANAADIQGKVIRVLDGDTIEILQNKTP
                     VRIRLANIDAPEKKQAYGRWSTDQLKSLIAGQFVRVTYSHNDRYGRIIGRVFTTGGSE
                     ANRFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHK"
     gene            complement(5312..6046)
                     /locus_tag="SI621_002306"
     CDS             complement(5312..6046)
                     /locus_tag="SI621_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HEI4065018.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKK
                     SRNPVYKLLNKALKEGFIIKHEIPSSKRKLTVWGITMQGLAMVVKADDITFPSYFEPS
                     KLKYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAI
                     MAIETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVM
                     RNNTPVPFDAKNREMFIFRTIDELEQ"
     gene            complement(6056..7783)
                     /locus_tag="SI621_002307"
     CDS             complement(6056..7783)
                     /locus_tag="SI621_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEI4065019.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNL
                     PREERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITI
                     LFTLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPL
                     DYIDLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGE
                     AVFVEDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAG
                     FSLSKKGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELE
                     EIALVNSVNAKNGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRI
                     NTTPRVVAIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSV
                     IGAVIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIR
                     QAESYYVDSNMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHN
                     NNDVPSSGL"
     gene            complement(8593..8898)
                     /locus_tag="SI621_002308"
     CDS             complement(8593..8898)
                     /locus_tag="SI621_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HEI4065020.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQ
                     ARLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAAIKAEDECLTEITRAQV"
     gene            complement(8926..9141)
                     /locus_tag="SI621_002309"
     CDS             complement(8926..9141)
                     /locus_tag="SI621_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065021.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVCSIPSGMKGNFITSIGETL
                     EDLKKRYPDIILITESEALALQSKREN"
     gene            complement(9144..9443)
                     /locus_tag="SI621_002310"
     CDS             complement(9144..9443)
                     /locus_tag="SI621_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEI4065022.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGE
                     CRSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
     gene            complement(9472..9870)
                     /locus_tag="SI621_002311"
     CDS             complement(9472..9870)
                     /locus_tag="SI621_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cag pathogenicity island protein Cag12"
                     /protein_id="HEI4065023.1"
                     /translation="MIKHILVTVLVTLITGCSSPPPPVPVEWNKKPVSLNEKLPEWNP
                     NSLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSAKNW
                     LYHHGANGVIIYHPITNCLTCNETTIYFSR"
     gene            complement(9867..10892)
                     /gene="virB11"
                     /locus_tag="SI621_002312"
     CDS             complement(9867..10892)
                     /gene="virB11"
                     /locus_tag="SI621_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HEI4065024.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELWTKIDGVW
                     GQHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTIS
                     ITIRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYG
                     KTLAVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSD
                     EKGSVVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHE
                     AIDGFITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNVRRMNDIYFRPLHRNQ
                     YYEEMKV"
     gene            complement(10882..11073)
                     /locus_tag="SI621_002313"
     CDS             complement(10882..11073)
                     /locus_tag="SI621_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065025.1"
                     /translation="MNCKDKETWLHMLAAGHMGGLAGNPNEAAAQDAYQRLIKNKTAQ
                     AVLQRCTTRITLKGANDDR"
     gene            complement(11057..12322)
                     /locus_tag="SI621_002314"
     CDS             complement(11057..12322)
                     /locus_tag="SI621_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEI4065026.1"
                     /translation="MTDENKSPSTEEEKPVMTSDQDDIATLEREARAKREAELQASQD
                     DEKENSLQPAVNKLKKRQKGKAAAFLAICAVALIGLTWGGNWVYQNFLKQENGEKTKE
                     VTPPKKTNDYRKRTDLGMATNEPENSTEGEQAVSQDNTGNVSGTAGQMKTFEPPQLNK
                     ASFLVRRNNTASPQNQVKTRQQEMILAAENNATQPTDSTATTTQHGTASQAISPATVR
                     RIPYNPDLYIPENTVIPCSMDYRFVSDRAGKIRCTVAKDIWSASGNTKLIEKGTTATG
                     IYQTGITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERF
                     GGALMVGMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKN
                     QGEIINLITGQDIDFSNIYTLRLKNELQR"
     gene            complement(12364..13269)
                     /gene="virB9"
                     /locus_tag="SI621_002315"
     CDS             complement(12364..13269)
                     /gene="virB9"
                     /locus_tag="SI621_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HEI4065027.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTV
                     VNTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTT
                     QVVIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKE
                     AAARQKEWEQKQEQTQIQKALRTAQTPRNWDYTKYPGKDSYNIEPDFAYDDGRFTFLG
                     FSPNKSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNG
                     FGKVHAADNSTVSDKVERVEKTDNT"
     gene            complement(13269..13952)
                     /locus_tag="SI621_002316"
     CDS             complement(13269..13952)
                     /locus_tag="SI621_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEI4065028.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLITILSLI
                     AIVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYV
                     YPALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATA
                     PDKVATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQ
                     TDREIRGGE"
     gene            complement(13945..14088)
                     /locus_tag="SI621_002317"
     CDS             complement(13945..14088)
                     /locus_tag="SI621_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065029.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPHAFSGQGSPVNTPQIIAELT
                     THD"
     gene            complement(14138..15208)
                     /locus_tag="SI621_002318"
     CDS             complement(14138..15208)
                     /locus_tag="SI621_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEI4065030.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYI
                     MWRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSST
                     SIWQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIA
                     EIMILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSR
                     MSEAVTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGI
                     GAAAYGAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEA
                     RMLTDQSAPKLPPSIKRLPPPR"
     gene            complement(15218..15445)
                     /locus_tag="SI621_002319"
     CDS             complement(15218..15445)
                     /locus_tag="SI621_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HEI4065031.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAEC
                     QKTGSASENCKHANEAHWQIQQLNAPEISFN"
     gene            complement(15457..16161)
                     /locus_tag="SI621_002320"
     CDS             complement(15457..16161)
                     /locus_tag="SI621_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEI4065032.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQ
                     WLQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLS
                     GGSSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLG
                     EINELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLADERR
                     KKWNQQQLNAPEIMFN"
     gene            complement(16176..18920)
                     /locus_tag="SI621_002321"
     CDS             complement(16176..18920)
                     /locus_tag="SI621_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEI4065033.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFL
                     PIEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDA
                     MKLNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLH
                     GLIRSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFV
                     TICYRPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTE
                     NEAVFSSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFR
                     GLEVKEFSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDA
                     VSQRLDLDVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVP
                     AEISLSAAYMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVR
                     TPSGDGYYLNLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDS
                     FSPDARTKRLTTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFI
                     KQLMKILCTRNGATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQ
                     ENGLSIRLSQWARGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLL
                     YRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIK
                     DEIAPAVIEQCGTQILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQF
                     RRGDTKRFAARITLDLSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL
                     "
     gene            complement(18933..19229)
                     /locus_tag="SI621_002322"
     CDS             complement(18933..19229)
                     /locus_tag="SI621_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEI4065034.1"
                     /translation="MINKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVS
                     NGLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
     gene            complement(19233..19937)
                     /locus_tag="SI621_002323"
     CDS             complement(19233..19937)
                     /locus_tag="SI621_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEI4065035.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPK
                     RERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLF
                     NPCTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSP
                     EKPRYVVPGTRDDIATQSAILNATPVEAPTRPRVVWPGAIVRGVPAQLRQKKADTVYY
                     PAQVIRGNRFSTEKED"
     gene            complement(19953..20159)
                     /locus_tag="SI621_002324"
     CDS             complement(19953..20159)
                     /locus_tag="SI621_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065036.1"
                     /translation="MPKPTYNVYVTQDVPTDENGGKNTYWTKVGAAFAHNGKPGLNIM
                     LVPGIAVSGKLVLLEPKEDAPQGN"
     gene            complement(20160..20372)
                     /locus_tag="SI621_002325"
     CDS             complement(20160..20372)
                     /locus_tag="SI621_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065037.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAF
                     LQYMIDKLSNEAHNEMLKELMSKEDK"
     gene            complement(20449..20928)
                     /locus_tag="SI621_002326"
     CDS             complement(20449..20928)
                     /locus_tag="SI621_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator NusG"
                     /protein_id="HEI4065038.1"
                     /translation="MGACVRKVTLMNAWYLLYCNNNDLDRIAYRCKTMNVVAFCPRYI
                     KITPRKDCNAVRKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPE
                     KVVIAIKCAKILALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
     gene            21153..21518
                     /locus_tag="SI621_002327"
     CDS             21153..21518
                     /locus_tag="SI621_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4065039.1"
                     /translation="MVPKRLKEARMEAGLSQEKLSQLLGVAEGINTRSRFSSYEVGRT
                     EPPFKLVKKIAELLDYPENYFYTEDDDFAKAVLDFHRKRTSPELNPNYDSEVEARRMA
                     SKLDNVKKIVAQLNEYLKE"
     gene            22076..22801
                     /locus_tag="SI621_002328"
     CDS             22076..22801
                     /locus_tag="SI621_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA protein"
                     /protein_id="HEI4065040.1"
                     /translation="MRAVVAEKNNSLIPRISPSGVAKQSPCYNVVSRKRRRFLNRWIK
                     SLTTVAGRIKIKNELRRRIRHNRYWVNEANKFGIEALCELMLAILDDLDLRSWETRHN
                     LETLAERAGLVTRSDAGHKSISRASRGCDRLVWLNAIITEKAPFNPYDARCACKHIEV
                     TEDFFAILGIPLKLVYRERARLLKADPDEVIYSGDARLIAIRVQNWTRKAAAGLARMK
                     AKRDAARQRKKEYYAHSPTPIPA"
     gene            23758..24030
                     /locus_tag="SI621_002329"
     CDS             23758..24030
                     /locus_tag="SI621_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065041.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIEVMQEQAVKAFVSWMTNQEG
                     TEFLSTLQEITTELDALIDAVESHSPIFRKKATELEALLAHYKTLL"
BASE COUNT         6334 a         5129 c         5254 g         7386 t
ORIGIN      
        1 tataaaggtg tgatcaggtt ggtcgagcaa cctgatcact ttatcgacac tcaaaacaat
       61 agagaggtgt tcaaatgtca acaggtcaga tcgtacaact tgcggattca cttgtcaaag
      121 gatgggaaac gagaatccca gcaatgaaat ttacggattg ggatcaattc aggtggtggt
      181 taaattattt acagggatat gaaatgtttt aagcaatgtt gatgtcctga cagtaattat
      241 aaattttttc aaaaagagag ctaaaaaact tgcaatacct gcaattgaag gtataattaa
      301 atcatcgaga gggaaatggt ttccctctca tcttagcccc acaggggcgg gagctacaaa
      361 atgaatatcc aagaagcatt aaatattttt ggcttgtctg gccagttgac tgaaaaagac
      421 atcaaaaacg cctttaagaa agctgcatta aaatatcacc cagaccgtaa tccggtaggt
      481 gctgaaatga tgaaggcggt taattcagca ttcgattttt taatggcgaa tcttgataag
      541 attaatcagt accaaagtcc tgaacaaagt gcaatatata actatggtga tgaactagaa
      601 aatgttttaa aggctcttta cggtttgtct ggtttagttt atgaggttat tggtaactgg
      661 atctggatta gtggaaatac tatcgagtac aaagaagcat taaaagaaat agggtgtaaa
      721 tgggcggcaa agaaaaaaca atggttttat cgtccagaag aacataaaag ttactggaac
      781 cgtgaagagc acacgataga ggaaatccgc gcaaaatatg gcacaagcgg aaaattccag
      841 gccggaggat ggcaacaagt tgaaggcagg gcaagagttg aagccagagc ataacgaaac
      901 gggggcgtaa gccccctata tcctcgccag caatcctttt cgggcatcga acccgaaaag
      961 gattgagtag ctgacactct gcgattgctg gctacgttag cgcagtaaag gatttgaaag
     1021 catgacaaca ttttcagaaa aatatcagcc tgtctttgaa gttgtatgcc gtattcttgg
     1081 gaacggctgg cgtgttaaca aactggataa ttatcagcat cgtattaaac tgacttctac
     1141 agaaattcgt aaatactcca tcattattcg tctggagcgt gaaagatttt ttatagttgg
     1201 tagcgttgat agtcttgtat ggcacggtaa tccttccgtc tgtactgtat ctcttacgcg
     1261 caatcctgtt ggtattgcta atgatatatc tcggaaaatc cttgctaatg cactggatga
     1321 aattgaattg ctcagggagc atgagagcgc attacaggaa aaacaagata aagtgcgaat
     1381 aatcaaaggg atgttatcac agttgctttc tatatctccc tggtatgggg cattaacggg
     1441 atttaatgcg gataatggac ttgcaggaaa aatactacaa aatggtgatg attatgaaat
     1501 ggttatcaga gggcttaatg ttgatcaact tatcaaagtg gcaggtttta taaaaaaact
     1561 ataacaaagt gttgatgtga attttatatg gggttgttta tgaaaactga taatgttttc
     1621 tttaaaagta agggtagtta ctcttatgtt gttacagtag atatggataa tcagatgtat
     1681 atggtcagaa agtatgttaa gacatatgtg aaatcttgtg ataaagagca agttaaaggg
     1741 aaaaagacag ttaaaaaatt ggtgctcgat acgaatctta cattgccaga attattgggt
     1801 cttgataatg gtattctggc tgatttattt acaagaggta ataacaatgc aaactaaacc
     1861 aactccggca gatgttaaag aggcgagaat ttccgcaggt ttaacgctta aggaagctgc
     1921 cgaaattttt ggctatcagt taaatacctg gcaaatgaaa gaaagcgcag gtaaagctac
     1981 aaggtcttta tctgtaggtg aatataacta cttattgttg ttggctggaa aacaccctga
     2041 atatgttctt tgtaaaaggg atacgatcac cgcagaacca accactgtct gaccccaaaa
     2101 ggggcttttc gccccttttc tactaaatgt taaaggccac tcgctttccg gtagggagtt
     2161 caacatctag gctcaacttt cctcccatag cttcaacgta acgctttaac gttgccagct
     2221 tcaaatcatt tccgcgctgt tccagttgag ttacagcggg ctgactgatt cccattgcat
     2281 cagcaacctg tttttgtgaa agctgcaatt cttcacgcat catttgcagc cctgtttcca
     2341 gaatcatctc atcagccata gctttgatga tcgcctgact ttcaggtgat tgagccgcaa
     2401 ttacttcttc taatgttctc attttttgcc ctccagttcg gctaagtgag tggaaaattc
     2461 ttcgtcagca atacgaatca ttctgtcgta aaatttctta tcattacttt tgtcgccagc
     2521 gcaaagaatg attgcttgtc taattggatc aaatgcaaag aatgccctga ttgggtgtcc
     2581 ctcatattgt atacgtagtt ccttcatatt cttatgacgt gaccctttca ctgtatctgc
     2641 atgtggccgt gaaagagagg ggccaaactc ttcaagatgt ttaagttcag ccagcacttt
     2701 tcgttttagg gctagttcct gctcttcaaa ccattcatca aataatctgc caaaataaac
     2761 agtccacatg tcccttttcc tataagttat agcttataga tgattgtata agctacgact
     2821 tataatttca agggaaacgg ggctatttaa cataatggac gttatcggca ccatcggatc
     2881 accgttcgtt gtcggatctg caaaatccgc tcaaaagatc atgttttcct gggcgttttc
     2941 caccgttttt tatgttgcgt atttgttaat aaaacgtcat tttttgtttt ggctgaattt
     3001 ggttgtgaaa gccttatatt cacaagttat ctcttcactt gccgcctgta gttcaaatag
     3061 ttgttgcaat tcttggcacc aatacggata ctataattat atttttctat aaaaatattt
     3121 ttataggggg tcgtatgacg gtagctattg cgctggttaa cggtaaaggc ggggtgaaaa
     3181 agtcctcaat gagcagggct attgccacag agtacacgaa ggcagactgg aaggtattag
     3241 ggcttgatat ggatgttgat caggggtcat tcctggattg gggacagcgg cgattgagat
     3301 ataaagttga accacctgtc actattcagg gatgcggaac ggtatcacag gctaaaaaac
     3361 atattgaatc agggatatgg gatttagtcg ttatcgatag tgctgcttat gcaactaaat
     3421 cgctgttgag cctggcagaa attgttgatt tgctcgtgtt accgtctggt tttagtgtgg
     3481 acgatttgag aacgacggtt acaaccgtga atgcattgcg taacaaggga cactcaacaa
     3541 ataagatggc tgtagttttt gtcggagtca gtgaaagcga aagtgagtat cgtaccgcgc
     3601 ttgaatatct taaaccgtgt ggtgttcaag ttatcaatgg cgcaatacct tttatgacct
     3661 cgttcagcca ggcacaggat aaaggactgg cattgacgga atcccctttt ccaggtccac
     3721 ggcagaaagc tgacgaagtt gtccagggag ttattgactt gctgactaaa ctctcacagt
     3781 gatatataat tctataatta tatttttata gaggtgataa gatggctaat gtaccaccaa
     3841 aaccgcgtaa caccaaaggc gagccgccaa gtctggagga tacacaacac ggatttaatc
     3901 cgacgcgtaa aaacgctgat gggagaaaag ctcagcttaa cctttatgtg gatatggaag
     3961 ttaaacggga gttccgctcg attgcagcgg ctaatgatat gaagcacggg gagttgctgg
     4021 aggcatacca ggaattctgg aaaaagcatc attaaaatgg cataggggac gaactgtccc
     4081 ctgatattaa cacttaccaa cataatgaat atgttcaacg gttcctaaaa tctcaaaacg
     4141 atcgggagct acctttcgcc agtgattttt ggcacgagag gcatagcttc tgtcccagcg
     4201 gagaatacga ttattgtcct ggataacaca taccatcctg ttttctgctg tacgcaatga
     4261 ttttgtaatt ttagctaaat cattatctgg acttagatca attagttttt catggccgtc
     4321 atttgacaca acgagtattt ttatcgtcat tatcgtctcc attatcatat tctggaaaca
     4381 ctattagtaa cgcttcgccg ttggtgcgtt tcataaaatt gaacactaat ctgcctactt
     4441 cttcatttgt catatagcgt tctattggca atgtttcgta ttgatccgca tagagaatat
     4501 tcacactttc ttcttctggt tctgaaatct ggccaattgt gatatctgtc agacctgtat
     4561 cagtgaaatt tcctgatttt atgtcaaagt cgctatcttt gaatttgtat gcatcctgcc
     4621 atacagcgaa acaagtgtgg caggtgatgg gcagttcgcc ttcagccagc ttttcacaat
     4681 tttgaaccag tactcgctct tggttgtact gtaagttata gccacctgta caaagcgttt
     4741 catacccatg agcgccagtt acgatatgtg tcgcaaccct ttccaggact ttcccattca
     4801 ctaataactg ttcacgtgtg gtaattgttt gcatgtcaat ctcctattta tgtcgccatt
     4861 cccacggtgg aacgggattg gggtcagccc acaaacctcg tttttgtgag cgagcttcct
     4921 gttgtagatc tgccagagcg ttatctgtgt tgtaatgctc atacacccat gcagcacccg
     4981 attgcaccat aaagcggtta gcctcactac cgcctgttgt gaatacacgt ccgataatgc
     5041 gaccgtaacg gtcgttgtgt gaatatgtca ccctgacgaa ttgtccggca atcaggcttt
     5101 taagttggtc agttgaccag cgtccgtaag cctgtttctt ttccggtgca tcaatattgg
     5161 ccagacgaat ccttactggc gttttattct gtaaaatttc gatggtatcg ccgtcaagta
     5221 cacgaataac ttttccctgt atatccgctg cattggcata tagtgaaggc gacaagatat
     5281 ataatgcgaa tatgattaat attttcatca actattgttc cagttcatca attgttctaa
     5341 aaataaacat ctctctgttt tttgcatcaa aaggcacagg tgtattattc ctcattacag
     5401 ttttgattga gtcgaacagt ctgattaatg attctttact tttggtgtca ggcatgacgt
     5461 acaaagcata atgccagttt cctttatctc ttgccgtaag atgactgttt ataatgctta
     5521 tatagcgagc acgagttttc attgttcgtt ctgtttcaat agccattatt gcgccagttg
     5581 gttgttttat tactccatca gggcgatggg aacagccgga ataacgtgcg atgaaggctg
     5641 cactatcacc gttaatccat tcagtagcgc ctttactttc cagagatatt ctcactcgct
     5701 gatttaaaag acgatgctca agagtccagt attttagttt gctgggttcg aaataactag
     5761 ggaacgtaat gtcatcggct tttacaacca ttgctaatcc ttgcatagta ataccccaaa
     5821 cagttaattt tctttttgat gatggtattt catgtttgat aataaaacct tctttaagag
     5881 ccttgtttag taatttatat actgggtttc tgcttttttt tgtgaatcct aattttcttt
     5941 ttataatgtg aaagtctgaa tacgtttcat ccttaaggaa atttaaaaga caaagcatgt
     6001 tgttagctga tattcttttt ctttcgtaaa aatcatgaat taacatagta aaacctcata
     6061 aaccactgga aggcacatcg ttgttattgt gttgttctat ttgttctgtc tcaaaagata
     6121 gcaattcaat ggtttctttg tttgctggta tatgagacat tttagttgct tttggtaaat
     6181 cttttgatgt gaacacaaat cctactcttt caggtagatt taaaaacata ttactgtcta
     6241 cataataact ttctgcctgt cttatcatgc ggtcagtatc aactttttct gttaatgata
     6301 agtcggttct caccttcctc atttcatcgt caactaatat tgaaccagtc attttagctg
     6361 cccactctgc tgtttctgga ttctggattt tatagattaa cttgaatttt gcgttttcaa
     6421 taacagcacc aattacagaa tcgccattaa gatcagaagg gcaatctctt aaatcatcta
     6481 ttgcctggaa ggccataaat atatgcacac ttttatctcg tgctgtacct aatccttcca
     6541 ttgtcgggcg tgaaatatga tatttcaact catcaagaaa aattgcaaca acacgtggag
     6601 ttgtgtttat tctgtctctg gtttcagcta tctgaatcaa acgcgtaagt atcatgcgtt
     6661 gtgcagaaat aattttttga tttcgggttg acccaatgat atagcagcat cctccattat
     6721 caaaaatctc tttaagagaa aatccatttt ttgcattgac ggaatttaca agagcaattt
     6781 cttctagttc acctaaaaat gctggggcat cttctcctat tgattttacg ttgtcatcgt
     6841 tgaataaatc atacagtgtc attccggttt tatgaattgc ggctacattt ctggctgcgc
     6901 ggcgatcagc tatacgataa aaatctgcgg cctctccttt ttttgataga ctaaatcctg
     6961 cattaaataa ttcttctaat tgttcatgtg atatgtcagc aagtaagtca agttgatggt
     7021 tggttctgtt taaattaatt acaacaaatt tctttcctgc ttttttacat gcttccctaa
     7081 gaacatgcac agcccattca tcatcttttg gatcttcaac aaaaacagcc tcgcctaaca
     7141 gaattgcctg ataaagcaaa accgatgcgg taatgccctt accggaacct gttgtaccta
     7201 tgattgctgc atgttgtttc tgaaattctt ttattgttat atattgcggt tggtcgtctt
     7261 tgtctaagcc aatgaaaacc ccttttttca aatcaatgta atctagggga ttgtattcta
     7321 tgctgtctgg caacaattct tttactttgc gaacatctgt tcttgtattt ctctcaagtt
     7381 tgcttttctt tatcattctg tgtttcaggc tgtctatttc acctgctaat aatcgtcgcg
     7441 cggtcaaatg aaaagctaat ccagctagtg taaagagaat tgtgattatc cataatggta
     7501 ttctaagtag tgtattggga tcgagaacgt caaataggta gctcagacca tgaatactaa
     7561 gtggtgacat tgtgccgaat atgaaaaaga aaatggacac gcacgcaaca gattttaacc
     7621 agaagggagc gttttttctt tcctctcgtg gtaggttgaa tataaatggt agtgttagtc
     7681 ctgccagtaa tgacaatatg acagggctgt tctgtagcca taacagaaat attgaaaagg
     7741 catcagcaat accattcagc ttttgcatta gatgtggatt catatttgtt ttccccattc
     7801 ataaagtgaa tctatggggt acgacaatgg ggtacagtaa tgggggatat aatgtacccc
     7861 atagctgtac cccattattg taccccaata aaagccaaaa acgttgtgat cgcctaaggc
     7921 gatcatgccc gttatccgtt ctttcagaag ccagccgata agttatcgtc tggctcctga
     7981 aagcccgaag aacgggcaac ggcgtgtgcc gtttcggcct ggctggaacg ccagtccttc
     8041 acgtcacatg ggggtaatgg cccgtctgga cgccggacca aaacccccaa ggtcaaaggc
     8101 ggcacaccgt cgcgcgctac gcgcgaccac cccctttaag acgcgcgttt agcctatttt
     8161 ttcttcgcaa gctcgaaaaa attggaacgc tgctttaaag ggggtggtcg cgcctaatcg
     8221 gtaatatccc ccgctggggc gggaaatatt acctggcccg acggtgtgcg gggcaagccc
     8281 cccacaaaaa ccggcacgtg ggcgtgcctt ttttctcgct ggggcggaaa aatttttgcg
     8341 gctccccccg gcccgcttca attcgcttgg ggcgaatgtt cgcgggtggg cgtggtgcct
     8401 gaaaattaac aatttcttcg aaattgaatt ttctgtccac gcaccacttt ctttattcct
     8461 gtcttaacaa tttcttcgaa attgaagcca gaaataaaga aagcctgaac tgcaccacgc
     8521 cctgcgtcgg actcgcccaa attcgcaaag cgaatttgcc ccgcccttcg ggttgctacg
     8581 caatcctctg cctcagacct gtgcccgtgt tatttcagtc agacattcat cctcggcttt
     8641 tattgcagca ttatatctgt cttctgttat ttcataacct tgtgaagcag cgtaactaat
     8701 cagcttattg gcatcagaac ttttcagttt tactgtgtgc tcaattaacc ctcgcattaa
     8761 cctcgcttga tatatttcat aatatagccc gcatcccctg tttgctttat tactaatatc
     8821 atcaaagact ctttttacat ggattctgtc ctgattcctt gaatgaatat tattaatgaa
     8881 catatcttct tttaacatag gattctccag atctgaaaga atatgttaat tttccctctt
     8941 cgattgcaat gctaatgctt ccgattctgt aatgagaata atatcaggat atcgtttctt
     9001 taaatcttca agcgtttcgc cgatagaagt aatgaaattc cctttcattc ctgatggaat
     9061 ggaacacact atcttgtgtg catagctata aacaagatcg gagtttgtca gataaaatac
     9121 catgttttca tctgtattca tatttaccct cgaactctac caaacttact cattattttt
     9181 gatatagctg cgggatcagc ggatgaacac tgatttaaaa atgctttacg tgcatcaaat
     9241 gttttactcc atcgtattga acctttcttt ttcttaagaa tgttgaagaa tgctttttct
     9301 gctgatctgc attcgccacc tccgctgttt cctgtagctt ttccgtacat acataaaaca
     9361 acctcgcatg ggtctgcggc ataagagtaa acaggtgtgg cggttgttaa aacaaacatt
     9421 gttattgctg caattttttt catgtcagaa ctctttgttt aatattttgt gttaacgcga
     9481 aaagtagatg gttgtttcat tgcacgtaag acaatttgtg atggggtgat atataattac
     9541 cccgtttgca ccatgatgat acaaccagtt ttttgcactg aaataatcag cgcctgactt
     9601 tgttgctact gtgatttgtg ttgagtgagc aatggcataa aagacagatg gcatccaatc
     9661 ttctttattg aaatcttgtg agctaatgga taacgtccat ttgtcattaa taacaggcga
     9721 ttttattatt aagctattgg ggttccattc aggaagtttc tcgttaagcg ataccggctt
     9781 tttattccat tccacgggta ctggtggagg cggtgaggaa catccggtaa ttagtgttac
     9841 aagcacagta accagtatat gctttatcat actttcattt cctcgtaata ttgattgcgg
     9901 tgtaacggtc ggaaataaat atcattcata cggcgtacgt ttccatctaa atcaatgctc
     9961 acaacaacat ccagagagtc gagaacttta cgtagcaaga atgaatatgg taattgcgca
    10021 cactgtgggt tttcagtaca gcgggtaata aaaccgtcga ttgcttcatg ggctgaacca
    10081 gcatgaatgg aggtcatact tccttcatgg cctgaccccg ttattttcag aaaatcccat
    10141 gcctctccac ccctcacttc tgttaataaa attctgtctg gattcatccg gtaattccag
    10201 cgtataagcg ccgctggtgt tacaacagaa cctttttcat cggatgactc agaaggataa
    10261 aacagatgaa catagttctt atgtttgtag aatattatct ctgggttgtc ttctatcgtc
    10321 gttatgcgta aatggacggg tatatattgt atcaacattt tcatgtatgt tgttttaccc
    10381 gtactggttt cacctgcgac ggctaatgtt tttccatact ccacacattt ttccataaac
    10441 aaaggaatgt tttttgattt gtataattca atcaattctt cgttatgtgt ggatgctttt
    10501 tcaatgcctg tgacgcggtt ataaaaaccg ctatcaatat atgcctgatg cggaatttga
    10561 atttttgacg gtttacggat cgtgattgag atcgtgtcac gttcgcacgc cggaggtacg
    10621 acaacctgac atcgttctcc tgattccagg gttgcagaaa gtcctggttt gatatcctcg
    10681 atattatcgt tatggtgttt tgcaagacac cgagcaaagt tatagcaatc gtcaaatttc
    10741 agatcaacgg tatgttgccc ccagacacca tctatctttg tccagagttc acctggacga
    10801 ttaacagcta tttctgtcag tcctggtaat tccagaaagt caccaaaaaa tcgctgttta
    10861 tagcggtcca gggaaatatt cttagcggtc atcgtttgct cctttcagtg tgattctggt
    10921 ggtacaccgc tgcaaaacgg cctgtgcggt tttgttttta atcagacgct ggtaagcatc
    10981 ctgtgcggcg gcttcgttcg ggttcccggc gagacccccc atgtgtccgg cggcgagcat
    11041 gtgcaaccaa gtctctttat ctttgcagtt catttttcag cctcagcgtg tagatatttg
    11101 aaaagtcaat atcctgccct gtaatcaggt tgataatttc gccctggttt ttgtagagcg
    11161 taggggggat attgatactg ttttccagcg tggttcgagc catatccgcc atagcctggc
    11221 ggctgttctc tgtatagtct gtattgcggt cttttttacc cgcattgttt gatgcccatg
    11281 cgccaatatc tgggatcatg ccgaccatta atgcgccgcc aaacctctcc cagaagtggg
    11341 tatcaatcca tccatcaaca cctgattctc caagctcgcc agcggcgttg gtatcaatca
    11401 gcggaatatc cagatatggt cgctgtcggg tgcgtaattt tgtcatcatg ataaaagcgc
    11461 gcccctgccc ttgcgtaatt cctgtctggt atattcctgt tgctgtggtt cccttctcga
    11521 taagttttgt attgccgctg gcactccaga tatctttagc taccgtacat cgtatttttc
    11581 cagcgcgatc ggaaacaaag cggtaatcca tagagcatgg aatgactgtg ttttccggta
    11641 tgtacaaatc agggttgtag gggattcttc gcactgttgc gggggaaatt gcctgtgatg
    11701 cagtgccatg ttgtgttgtg gttgctgtac tgtctgtagg ctgtgtggcg ttgttttccg
    11761 cagccagtat catttcctgt tgtcttgttt ttacctgatt ttgggggctg gccgtattat
    11821 tacgacgaac aaggaagctg gccttattca gttgtggtgg ctcaaatgtt ttcatctgtc
    11881 cggcagtacc ggatacattg cccgtgttat cctggctaac agcttgctca ccctcagtcg
    11941 aattttccgg ctcattggtg gccatgccta aatcggttcg tttccggtag tcattcgttt
    12001 ttttaggtgg tgtaacctcc tttgttttct ctccattttc ttgcttcagg aagttttgat
    12061 atacccagtt accgccccat gtcaggccaa tcagggctac tgcgcatatt gccagaaagg
    12121 ccgctgcttt ccccttctga cgttttttca gtttgtttac tgctggttgt aatgagttct
    12181 ccttttcatc gtcctgtgat gcttgtaatt cagcctcacg ttttgctctg gcttctcgtt
    12241 ccagcgtggc gatatcatcc tgatcagatg tcatcactgg cttttcttct tccgtagagg
    12301 gggatttatt ttcgtcagtc atatatgagt ccttatataa aaatataatt atatttttat
    12361 agattaggtg ttatctgttt tttcaacgcg ctccaccttg tcagatacgg ttgaattatc
    12421 agccgcgtgt acttttccaa agccgttatt tttcagcccc actacggcat caccggaacg
    12481 taatacaagc cgtggtgtga tttcctggat aacgagaacg gtaaaattgc ccttacgctg
    12541 tatactgctg ttaaccacgt attcctggcc gttctgttct ttggtaactg atggaactga
    12601 tttgttgggt gagaatccga gaaacgtaaa gcgaccgtcg tcgtaggcaa aatcaggttc
    12661 aatgttgtaa ctgtcttttc ctggatattt cgtgtagtcc cagttacgcg gggtttgtgc
    12721 ggttctcagt gctttctgaa tctgggtttg ttcctgcttt tgttcccatt ctttctgacg
    12781 cgctgctgct tccttattag ctctgttacg ttcgtctcct ggatagcggt aactcaccag
    12841 gtatgcgggc tgttgtggcg atttttcatc aatgacattc agctcaacgt tgtacaggcg
    12901 cttatttgtc acaatcagca tattggtatg ccagtcacgg ctgttcggcg ggataacaac
    12961 ctgtgttgta ttaccgtctt caccaggtgc gccctgtgtg agcgcaaccg gacgaatgtt
    13021 taccctgttt gcatctgctc tggcttccca tgcttcatca aagccaggac tggcactgat
    13081 aacagcttca tcgttatcaa aaaccagagt cgtcataaat cctggttttg tgtttacaac
    13141 ggtaacattc tgcgggttat aaatgacctg ctggatacgt gcatcataac ggctcgcttg
    13201 tggtgtagct gcggcccatg cctggctgtt cagcattatt atcagggtaa ttaacgtcag
    13261 ttttctcatt actcgcctcc cctgatttcg cggtcagtct gccagcttgt tacggtgaat
    13321 ccgaacgggt ttatttcccg ctcaacatct gacatttctt tttcagggtc tgaatggaag
    13381 gtgaaacgtg catcccagta ttcattgcga atactgttat cagccatgcg acgaattatt
    13441 tttttataac gaacggtggc tactttgtct ggtgctgttg cattcgttat ctgctcagaa
    13501 ataatttcaa ttttcacggt gtgagccgcg ttattataaa ctttgtctgg tgcgtttttt
    13561 ccggcgtaca gtgccaggta gtcgtcgttt acctgtggtg agttgtagat ctgtactgtc
    13621 tcgtaatcgt cctgtaatgc aggataaacg taccgttcac gtaatttaac gtaattggcg
    13681 gccatagctt tttggtaggc ttcttccgct gtcagggctg tttttgatgc acgggtaacg
    13741 tgttccactc gtccggtatg attatctacg gtatagagtt caactgttgt ttgtttaagt
    13801 ggcaacataa tcactatcgc gatcagtgac aggatagtga ttaataaccc tgctccaccg
    13861 accagaaagc ctgcttttgt ggcgcgttta tccctgtcaa tcatttgctg ctcaaacgag
    13921 cgggaatcag caattaattt ctgttcagtc atgtgtggtt agctccgcga taatttgggg
    13981 tgtgtttaca gggctaccct gaccgctgaa tgcgtgcggc agagatttag atttttgtgc
    14041 gcagccagaa agcacgcaca gcaaagccag cgcgattaat cgtttcattt tatgtacctt
    14101 tttgtatttc tataaaaata taattatatt tttatagtta tcttggagga ggtaaacgtt
    14161 taattgatgg cggtagtttt ggtgctgatt gatctgtcag catccgcgct tcattacgcg
    14221 ctatcatttg ttctatagcg tgtttacgtg catcagcagc gttaaatact atgcgcgatt
    14281 gtggatctcc taacccttta ttacttgggc ttgctacttc tttaggtaat acttttcctg
    14341 cctcctttaa catctttcca gcgccgtatg cagcagcgcc aatgccgaga gccgccgcac
    14401 cttgcatagt ggctgtagca cttgctcctg caagtgcccc agctagtttt gcggacatgt
    14461 acaccagtat tgcagcgcat actccggcaa gacaaacttg agcgccaagt tctacaatgt
    14521 ttgctttgct ggcttgggtc actgcttctg acattctgga tgtaagaaaa ccaattacaa
    14581 ttcgcagagc taaggctgaa aacataattg ttagtattgc agcaaatata ttttgaagcc
    14641 agttattaaa cattggccgt aaaaagccat acatcaggca aaatataaat attggtgcgg
    14701 taatacctaa caataagatc atgatctccg caatcataga tacgaatgct gtgataatca
    14761 ttaagacaaa gacaccaccc catacaaata attcagcaat tgtgccttcc tctttgataa
    14821 attcggagtt gtctagatca tgtaacgttt tacctaaaac tcttgcgctg ttccatagag
    14881 tatcaagtaa ttgccagatg cttgtgctac tggaaaaacc ttcttttatt ccatttacag
    14941 catcaatgac accatcaaga tatccgttaa tgtttgctac aaaggataaa atgatagcca
    15001 tcctcattat atcccacata acatcttcca ttggtgtaga cagctttccc gctaaggttt
    15061 gatatcctcg ccacataatg tagatagtgg ctgacgtagc cgccagcccc atcatcattg
    15121 acgaataaga catcatcaaa ccattagtcg cgtcagttat cccaccgatg atattttttt
    15181 cgacagaaga aaatataccg gccatgataa acgcccttta gttgaaactt atttcaggtg
    15241 catttaattg ttgaatttgc caatgagctt cattggcatg tttgcagttt tcagatgcgc
    15301 tacctgtttt ctggcattca gcataaacaa ctttgagttc atcaggatgt tcacgatacc
    15361 attttgttgt tttagcttct tccttacatc ctgaagttaa cgccatcagg cctgaaataa
    15421 ttgtaaataa aatgattttc ttcattaagt cacctgctaa ttaaacatta tttctggtgc
    15481 gtttaattgc tgctgattcc atttcttacg gcgttcatca gcaagcatct tttcacgctt
    15541 ctccgccgct tttacgttca tttcccattg ggtcgtgagc gtatttagca tcacagattt
    15601 taactgaata ctgtttgcta agtcttgggc ctctttcata tctttggcat ttgctacacg
    15661 gtctgataat tcgttgatct caccaagcat ctcaccaatc tgggtttgta cttcattggt
    15721 ttgttcgatt tgtattgctt tgttgatgac ctcttgtctg catgtattgg cataactttt
    15781 tgattgttca gcattgcagt tatcgaaaat cttgtatttg ttgtacagtt catcaaattg
    15841 gccagaagaa ccgccggata agagcaaatc gttcagggct tgccctggct tacgtagttt
    15901 atccaggtca gctttaaggc ttttggcctg attaacgaac gcctgaatgt cacggatgcc
    15961 tgtaactgtt tccagttgtt tttgatacgc ctgtagctgg tctttatagt gttgtaccgt
    16021 ctgtagccat tgattggctt cttttgtcca ctggcgaata ttctctagtc tggctgttgc
    16081 atcaaacacg ggtataccag cagcgtttac tatcgggctg gcaataagaa ccgccagaat
    16141 aagggggcga aatcttttca tcgatatctc cttggttata gtgctttagc taggtaggaa
    16201 tccagccagt cgctgggctt cattccctcc tgatagagtg attcgaatat ttccaggtta
    16261 ggtgcgtcgc cgctcattac ttttgtgtat ctgccgatac cagacagatc cagcgtgata
    16321 cgggctgcaa agcgttttgt atctccacgg cggaactgat ttttgacaac gacatactgt
    16381 cgcgcctgtg gatcaagtcc tttaatgaca tcaaacactt ctggttcaaa tttcattcca
    16441 tccacgtaat gagaacgatc tgcgcttggg ttggcggcca gtatttgagt accacattgc
    16501 tcaataactg ccggagctat ctcatccttg attatttccg ctggagattg cgtgccaaca
    16561 accagcatac cgttaagttt tcggatagtt ttcagcttgt tatacgcaaa atccttaaat
    16621 acagggtcgc gtaaccactt ccagaattca tccatgaaaa taacaagtcg tctgccatcc
    16681 agcaggctgg ttatccggta cagcagataa aacgagatag gggcgcagac attcgggtca
    16741 tccagaaatt cagtcccgtc aattccgaaa ttatcgcagt tgctgatatc gaaagtgtcg
    16801 tgttcgttat caaacaccca accaaattca ccaccgcgcg cccactgtga aagacggata
    16861 ctcaggccat tttcttgcgc ttcacgcgtt gccggttccg gtaggttctc cagcattcgg
    16921 gttatgccgt agacgcgata ttgaggctcg tcactcatga cggcatttac agcatcattg
    16981 aggcgacgtt catcacgtgg cgttatggtt gcaccgttgc gggtacaaag gattttcatc
    17041 agttgcttaa tgaaattgat gtttcgctta gtggctggca gagcaaacgg attccagcct
    17101 gttggctcac cgctgaatac cctgtagtac tttcctccaa gtgcgcgaat gttgatttca
    17161 gcaccacggt ctttatcgag gtaaactgtg gttagtcgtt tagtcctggc atccggtgaa
    17221 aagctgtcat cacggttata tttttgctga ttgatttgta aaaacgtcat cagcattgtt
    17281 ttacctgagc cgtttgtacc cagaatgcag gtacttgcag gatttttttc gttgaactcg
    17341 tctttatctg ccagagtgtt atgcagattc agatagtagc catcacctga cggggttcgc
    17401 accagagcca tcgcgtcacc ccacggggct ttgtcgcgtt ttcctggata gaaattatga
    17461 agtgatgcca gttcaacaaa gttcaggcta ctcaactctc ccgctcgtgg ccgcagattg
    17521 taattgcctg gtaattgagc catataggcc gcagacaatg agatttcagc aggaacaggg
    17581 gttatcccta tattgttgag tgcattgctg atctcgtttg tgtctgcaat cagactttcc
    17641 agtgacggcg aaaacaccat aattgataaa tgatgtttac cgtaggcgat tttaccggag
    17701 gtgagcaggt caagcgccac atcgagatcc aaacgttgag atacagcatc atcatcagca
    17761 ctcatcatca gccgtcgggt gcgcttaatc gcctttttcg cttcctcacg ggacatgcag
    17821 gtataggatt gtgtaatcac ataatcgcac ggcgcgtaca gtaaggagtc tgtcatgccc
    17881 gttgcggttt cttccgaaaa ctctttaacc tccagaccac ggaaatattg agtgccatta
    17941 acatgattta tttgccctga ttcggcggag aagtacagcg caacatttcc cattacgttg
    18001 taagcggggg tacgtgtaac tctgactttt cgccattgat gcgtgagaag gtattcataa
    18061 aatgccagtt gggacgagaa tacagcctcg ttttcggtga aagtaccgag cgcgtgagca
    18121 ccataacggg ataaggctgt atccagcgta cttttgattt ccagcacttc aagtaatgcg
    18181 ctgtctatct cttttagctt ctgaccgtct ttcatacgtt tacggtttgc cttatccaga
    18241 ctggcaaacg gacgatagca gatggtcaca aacaggcggt tacgtcggaa tggcctttta
    18301 ttcagtgatg cgtaatagcg ttcgctcacg gtatccgcgt aagggtttcc tgaaacctta
    18361 tgcagtgagt ccgtaaactg ttcacggctg ttatgtacat aaaatgtaac cggaaagcct
    18421 tcaaatgagc gtattagccc gtgcaactgg tttgtaccga actgtagtgt ctcgtcagat
    18481 tcacaatcaa acggcgttcc tgtcagttcc caggtacaga aaaaatcgct attcagtgaa
    18541 cggacaacat tagggtgaat atgtgacgag taaggaatgt atttttttat ggtggcacat
    18601 tggtttagct tcatcgcatc aataaactcc tggatatcaa cagcattatg ttcaacaggc
    18661 ataaatgcgg ttgcgccaaa taaacggttt gctaccggat taccccttac ccttagccag
    18721 acagctatca ggttaaatct cagaggttca tttctggtta gtgatttcat aaccagaaac
    18781 tcaataggga ggaagagaag tagggctatg gaagtccaga tagcaacaat aattgtcacg
    18841 cccacgacag tcagcagggg caccattggc accccccaca gcgccgccgg acgtttcatc
    18901 gccttaagca gctttgccat atgttcggcc tcttagctca ttagcagagc gccaatttca
    18961 ctgcctccgc caattaaaat gccgccaatg atgatgtaac cgcactgcga caggctttcg
    19021 cctttccaca gagtcttata gccaatccag atcacagcga cggtgatggt gacggctgcc
    19081 agaccatgca gaccattcga aaccttttcc atgatggtat ttgctcggct gaaaccgtca
    19141 gccgcgaaaa cgggactgac aacacttact aacggagcca gaatataagc agatgcgcgg
    19201 ctgcaaatgg cctttaattt gttaatcatc ggttaatcct ctttttcagt tgaaaatctg
    19261 tttccgcgta taacttgtgc ggggtaataa acggtatctg cctttttctg gcgcagttga
    19321 gcaggaaccc cgcgcacgat tgcgccaggc cagacaacgc gagggcgtgt cggagcttca
    19381 acgggtgttg cattcagaat ggccgattga gttgcgatat catccctggt tccaggaacg
    19441 acatagcggg gcttttcagg tgagtaacca attcgctgta tgtaactggt tctggataac
    19501 gcagcttctg gttgttgtcc tgtagtaaaa tttccagagt aataacaact cagcgcccgt
    19561 ttcagtgtgc cgcctcgctg gtagcaatca gtgatgatct tttcaaaaac agaaagattt
    19621 gtgcacgggt tgaacaggtc agcagccgtt acggcgtaac tattaaaatt tgtacttgtg
    19681 atttgcatca gaccaacaga atagcgacga ccttttgcct ctatctgttt gacgatgctt
    19741 aatgcatcct ccttagtttt tggcatatgt gagataaagc ccttgtcgcc tggctggcgt
    19801 tcacgtttgg ggatgatctc agcaatagcg tagggattaa aaccagactc gacccgcgca
    19861 acatccagtg atgtggaggg gtgaactgtg gcggcacact gcatagccac cgccataaat
    19921 gaaacggtgg aaagcatagt accctcgcta aattagtttc cttgtggggc gtcttcttta
    19981 ggttcgagta gtaccagctt gccggaaaca gcaataccag gaacgagcat aatattcagt
    20041 cctggttttc cgttatgcgc gaatgccgcg ccaaccttgg tccagtaggt atttttaccg
    20101 ccgttttcgt cagtgggaac atcctgtgtg acgtatacgt tataggtggg tttgggcatt
    20161 tatttatcct cttttgacat tagctctttc aacatttcgt tgtgagcttc attacttaat
    20221 ttgtcgatca tgtactgcaa aaaggcactt ggacgaatat ttacctccgc aacataactt
    20281 atatctctgg cgtgttttgc cagtgtttca tagcgttttt tgccgatgta aataacggct
    20341 gtagatttag cctggcctat agggttcggc atgagtttat agctcctgta tgaaatatcc
    20401 gtgtgcaaaa gcacagcggt acgactggag aaggaatgaa ggtctggttt actgattctg
    20461 taaaagcctg gagaaggccg tttgacgttc taacggtgag atgattgaag atatgcgttg
    20521 aatttctgcc agtaggtcac taccgatgtt acggcactct atggaatcat catcaggatt
    20581 tagcgccagt atcttggcgc atttaatagc gataacgact ttttccggta cagtacatat
    20641 tgtggaaccg aaacttacga agccaattgc acctggaata gaggttattg ctgatgtgtg
    20701 cgtgacattc acatcaaaat ggaggaagag gtagttagga aataaaactt tttcttcctt
    20761 gcgtactgcg ttgcaatctt tccgaggtgt gatctttatg tatctcggac agaaagccac
    20821 aacgttcata gttttacaac gatatgcaat gcggtctaaa tcgttgttat tacaatatag
    20881 aagataccat gcattcataa gcgtcacctt acggacacaa gcccccattt tccgccgtcc
    20941 taagttgccg ggcttaggct ggcgtactca ttaagtgtta actgtaaaaa tacacagttg
    21001 tgattttata cagcaattct tgcgtgtcaa tatcaagaat ctaaagaaat aatagcatta
    21061 ctcgattcaa aatcaacctc gattcattat gttgactttg aggcctgtaa cgccctgtaa
    21121 gatgggattc ccaaaaaaag ggaggttgta ttatggtgcc taaacgactg aaagaagcga
    21181 ggatggaggc tggattatct caagaaaaat tatctcaact tcttggtgta gcggaaggta
    21241 ttaacactcg atctcgattt tcaagctacg aggtaggcag aactgaacca ccttttaaac
    21301 ttgtaaaaaa aatcgcagaa cttcttgatt atcctgaaaa ttatttctat acagaagacg
    21361 atgattttgc aaaggctgtt cttgattttc accgaaaacg tacgagtcca gagctaaacc
    21421 ctaattacga ttcggaagta gaagcaagaa gaatggcatc taagctggat aacgtaaaaa
    21481 aaatagttgc tcaattaaat gaatatctaa aggaatgagt gcttaggcat cagtgcgtat
    21541 gtgctccatt tggtcaagtc gcttttccgc ttcctcaccc actcgctcac taacgttcgg
    21601 tcgctagggt tgtggcgagc ggtaaatacc ctattaaatt ccgttatttt tccgtttgtt
    21661 atcgccgatt tcttttcgat attaatgcta atgtatgcgt acattagcat taatatcgtg
    21721 agttatatgc tgtacagtat ttaatctcaa ttttgttaag aattatataa ccagatgaag
    21781 tttaaagaaa aataggaagg taatcggcgg gatcttttgt ggatgaattg atcttcgaat
    21841 gggttcatgc tattttcgtg cggtttttac ggatttttgg gtgggacgtt cccgtaacag
    21901 cgtctagggg aagggggggg aagcatttta gcccccctga atgcaacaac cccctgatat
    21961 ctctcttcga ttttgccggt ccaagaagat aatcaggggg tccaaagcag gctgggtgcc
    22021 tgtaaaggat tatagcaaat gcatttctca atacaagacc tctgtccggc aataagtgcg
    22081 ggcggtggtg gctgaaaaga acaactcctt aatcccccgc atatcgccgt caggggtggc
    22141 aaaacaaagt ccatgctata acgttgtgtc gcgtaagcga cgccgtttcc tcaatcgctg
    22201 gattaaatcg ctgacgacgg tcgcagggcg tatcaaaatc aaaaacgaac tccgccgccg
    22261 tatccggcat aaccgctatt gggttaatga ggctaacaag tttggcatag aggctctttg
    22321 cgaattgatg ttagctattc tggacgatct cgatttacgc tcgtgggaaa ccaggcataa
    22381 ccttgaaacg cttgccgaac gtgccggact ggttacacgc agcgatgcgg gacataaatc
    22441 catttctcgc gccagtcgtg gttgtgatcg cctggtttgg ttgaatgcca tcattacaga
    22501 gaaggctccg tttaacccgt atgatgcacg gtgtgcctgt aaacacattg aagtaaccga
    22561 ggatttcttt gccatccttg gcattccgtt aaaactggtt tatcgggaac gtgccaggct
    22621 gttaaaggca gatcctgatg aagtgattta ctccggtgat gccagactta tagctatcag
    22681 agttcagaac tggacgcgga aagcggcagc cggattagcc aggatgaaag caaaacgcga
    22741 cgcagctcga caacgcaaaa aagaatatta cgcacattca cctaccccca ttccggcctg
    22801 atgctggcta cttaccgcct tatggcggta tatcgtcacg tccataataa atctctgtac
    22861 aaaaaatagt cttagcgtga gatatttccg ttttccaagc ggctccctgc tcaggaaaga
    22921 gcagccatag gttatggcca gagggtaaac gatgtacagc ttgtggataa tcataagtga
    22981 agtgcaaacg atatgacgca attactcttt tgcttctctt ttagttactt acgtactaaa
    23041 agagaagcaa aaagaaaaac attactgtta tcccctttta caagtggata actgaaaaac
    23101 atcggcgcaa gcgcctcaaa ggtagcaccc ctcgcaagca tcggggcatt agagacacca
    23161 tatccggtca ttaaaaacga acaatcattc acctatcatc gctgtaacat cgacggtcta
    23221 cgaccaccta ccacctaagt agctgtaagt tccgtgcaat tttggcttag cagggggctg
    23281 acgcggttcc tggcgacatg aacgtccttg attaggggta tcggcgcgcc ttagcatttg
    23341 tgattctatg gaactatatt tcgcgcctta catgccgcta acgcggcatc ttcccaccat
    23401 cgagcataac ttaactaact gctaacaata tgatcgagaa agatcacaaa aaggttcctg
    23461 ttgggggtca gtttggaaat cggaaatttc ggcgtttccg gtgtacaacc ctaggaaagt
    23521 agatccgctc gccgcaaccc tagcgaccga acgttagtga gcgagtgggt gaggaagcgg
    23581 aatagtgccc ttgctgaatt gagcacatac gcgaaggaat taaaggtgag tggtggaaaa
    23641 tttttcagtt cgaatagtga aaaatagttg caatacctgc atatgcaggt ataataaaat
    23701 catcgaaagg gaaataggtt ccctttccac ttagccccat aggggcggga gattaaaatg
    23761 agtgcattta tttttagaac cacatttaaa gcaactggcg atcagcaaaa agacgctatt
    23821 acccaaattg aagttatgca ggaacaagca gtaaaagcct ttgtttcctg gatgaccaac
    23881 caggagggta cagaattttt aagcactttg caggaaatca caacagaact tgacgcactg
    23941 attgatgcgg tagagtccca ttcccctatt ttcagaaaga aagcgactga actggaagcg
    24001 cttttagccc attacaaaac gttgctgtaa gtcctcaacg gggcaattgc cccgttatat
    24061 ggctctttat ttaatccaaa tctaaagcaa gtggagttta aaa
//
LOCUS       DARNVM010000065        24036 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.065, whole genome shotgun sequence.
ACCESSION   DARNVM010000065 DARNVM010000000
VERSION     DARNVM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.065"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..638
                     /locus_tag="SI621_002330"
     CDS             <1..638
                     /locus_tag="SI621_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065042.1"
                     /translation="EKSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDK
                     PGSEQRYTFKNTNRIYLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGW
                     WFKPFFAKRYTDQTYYTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAG
                     NGGKEGLNGAVALWWNATSYITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(676..1602)
                     /locus_tag="SI621_002331"
     CDS             complement(676..1602)
                     /locus_tag="SI621_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4065043.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            1692..2690
                     /locus_tag="SI621_002332"
     CDS             1692..2690
                     /locus_tag="SI621_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEI4065044.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKHQTEQLQAQQESRANKA"
     gene            complement(2687..2905)
                     /locus_tag="SI621_002333"
     CDS             complement(2687..2905)
                     /locus_tag="SI621_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEI4065045.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2907..4922)
                     /gene="ligA"
                     /locus_tag="SI621_002334"
     CDS             complement(2907..4922)
                     /gene="ligA"
                     /locus_tag="SI621_002334"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEI4065046.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGILEGGELPNTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLTQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4993..5991)
                     /gene="zipA"
                     /locus_tag="SI621_002335"
     CDS             complement(4993..5991)
                     /gene="zipA"
                     /locus_tag="SI621_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEI4065047.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6221..6982
                     /gene="cysZ"
                     /locus_tag="SI621_002336"
     CDS             6221..6982
                     /gene="cysZ"
                     /locus_tag="SI621_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HEI4065048.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7167..8138
                     /gene="cysK"
                     /locus_tag="SI621_002337"
     CDS             7167..8138
                     /gene="cysK"
                     /locus_tag="SI621_002337"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEI4065049.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8522..8779
                     /gene="ptsH"
                     /locus_tag="SI621_002338"
     CDS             8522..8779
                     /gene="ptsH"
                     /locus_tag="SI621_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HEI4065050.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8824..10551
                     /gene="ptsI"
                     /locus_tag="SI621_002339"
     CDS             8824..10551
                     /gene="ptsI"
                     /locus_tag="SI621_002339"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HEI4065051.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTTDA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10592..11101
                     /gene="crr"
                     /locus_tag="SI621_002340"
     CDS             10592..11101
                     /gene="crr"
                     /locus_tag="SI621_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEI4065052.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11143..11994)
                     /gene="pdxK"
                     /locus_tag="SI621_002341"
                     /gene_synonym="thiJ"
     CDS             complement(11143..11994)
                     /gene="pdxK"
                     /locus_tag="SI621_002341"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HEI4065053.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12099..12467
                     /locus_tag="SI621_002342"
     CDS             12099..12467
                     /locus_tag="SI621_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HEI4065054.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVSAQ"
     gene            complement(12470..13381)
                     /gene="cysM"
                     /locus_tag="SI621_002343"
     CDS             complement(12470..13381)
                     /gene="cysM"
                     /locus_tag="SI621_002343"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HEI4065055.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13515..14612)
                     /gene="cysA"
                     /locus_tag="SI621_002344"
     CDS             complement(13515..14612)
                     /gene="cysA"
                     /locus_tag="SI621_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HEI4065056.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14602..15477)
                     /gene="cysW"
                     /locus_tag="SI621_002345"
     CDS             complement(14602..15477)
                     /gene="cysW"
                     /locus_tag="SI621_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HEI4065057.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15477..16310)
                     /gene="cysT"
                     /locus_tag="SI621_002346"
     CDS             complement(15477..16310)
                     /gene="cysT"
                     /locus_tag="SI621_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HEI4065058.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16310..17326)
                     /gene="cysP"
                     /locus_tag="SI621_002347"
     CDS             complement(16310..17326)
                     /gene="cysP"
                     /locus_tag="SI621_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HEI4065059.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17497..18288)
                     /gene="ucpA"
                     /locus_tag="SI621_002348"
     CDS             complement(17497..18288)
                     /gene="ucpA"
                     /locus_tag="SI621_002348"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HEI4065060.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18417..19274)
                     /gene="murR"
                     /locus_tag="SI621_002349"
     CDS             complement(18417..19274)
                     /gene="murR"
                     /locus_tag="SI621_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HEI4065061.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            19438..20334
                     /gene="murQ"
                     /locus_tag="SI621_002350"
     CDS             19438..20334
                     /gene="murQ"
                     /locus_tag="SI621_002350"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEI4065062.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            20338..21762
                     /gene="murP"
                     /locus_tag="SI621_002351"
     CDS             20338..21762
                     /gene="murP"
                     /locus_tag="SI621_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HEI4065063.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21767..23068
                     /gene="pbp4b"
                     /locus_tag="SI621_002352"
     CDS             21767..23068
                     /gene="pbp4b"
                     /locus_tag="SI621_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HEI4065064.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(23126..24025)
                     /gene="yfeX"
                     /locus_tag="SI621_002353"
     CDS             complement(23126..24025)
                     /gene="yfeX"
                     /locus_tag="SI621_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HEI4065065.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLYNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
BASE COUNT         5660 a         6510 c         6088 g         5778 t
ORIGIN      
        1 ctgagaaatc gtcgaccaaa tcacacaaag atgattttgg atatctggaa ctggaaggcg
       61 gcgctaactt tagttgggga gaaatgtacg ggttcttcga ctgggaaaac ttttataatg
      121 atcgtcatga taaaccgggt agcgagcagc gttatacatt taaaaatacc aaccgtattt
      181 acctgggtga caccggattt aatctctact tgcatgcgta tggcacctac ggttctgcga
      241 atcgtgtgaa tttccatgac gatatgttcc tttacgggat cggctacaat ttcaccggca
      301 gcggttggtg gttcaaaccg ttctttgcaa aacgctatac agaccaaacc tattataccg
      361 gcgataacgg ttatgtcgcc ggttgggttg caggttacag ctttatgctg ggcagtgaga
      421 aattcaccct caccaactgg aacgagtacg agtttgatcg tgatgccacc tacgcggcag
      481 gtaatggcgg caaagaaggt ctgaacggtg ccgtcgcact ttggtggaat gcaacttcat
      541 acatcaccac agggattcag tatcgttatg cggatgacaa actgggcgaa gatttttacc
      601 aggatgcgat aatctactcc atcaaattta atttctgatt gattactgcc ggggctcccg
      661 gcagtatgaa tagctctata tctgatacag cggatcccct tcccgcccag ccattagcgc
      721 acggccctgg cctcgtacca cgtcccataa cgcttgtgcg gcggtggaaa gtgaacggtt
      781 cttcctgcgc accagcatca actggcgttc caccaccggc gtaatgcgct ttaccaccag
      841 cgggctacct tcaggcaatg gtagcgccag agcgggaaga atactgatgc cgatgcctgc
      901 ggctaccatc gggaacagcg tcgccggatg accaatctcc tgcacaatat tcgcctgaat
      961 accgttgcgc gccagagctg cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa
     1021 caccaatttc gctccctgta atgcctgcca gggtacataa tcttccgcgg ccaaagcgct
     1081 atcgcgatgg caaagcaaga agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc
     1141 cactgggcct ggatcgatga cgatgccaaa atcgacatct ccctggcgaa tactttccat
     1201 cacccactgc tgcggacgat cgtgcaggat aaactgaata tctggatagc ggcgatggct
     1261 ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc tggctggcag cgacccgaac
     1321 ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc
     1381 cagcagtcgc tcaagacgca aagccaactg ctgccctgca tctgtaagca ccacttcacg
     1441 cgtggttctg tccagcaggc gaacaccagt atgattttcc agctccttca cactgtgact
     1501 tactgccgac tgactcaggc cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc
     1561 tactgtgacg aaaaccttta attgctttaa agaataattc atctgttttc ttcatgaatg
     1621 gatgcaataa atcaatttta tttctcaaac tggaagaagc acaatagaac catcgatcat
     1681 ctggagtctt tatgaaactt tttcgtatcc tcgatccttt caccttaacc ctgatcacgg
     1741 tggtgttgct ggcctctttc tttccggcca gaggcgattt cgtccccttc tttgaaaatc
     1801 tgaccaccgc agctattgcc ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgaag
     1861 cgattattgc tggcggtggt cactggcgac tgcatttgtg ggtaatgtgc agcacctttg
     1921 tactgtttcc gattctgggc gtgctgtttg cctggtggaa accggtaaat gtcgacccga
     1981 tgctctactc cggttttctc tacttgtgca ttctccccgc taccgtgcag tctgcaatcg
     2041 cctttacttc aatggcgggc ggtaacgtcg cggcagcggt ttgttctgcg tcagcatcca
     2101 gcctgttggg gattttcctt tcaccattgc tggttggtct ggtgatgaat gttcacggtg
     2161 cgggaggcag ccttgagcag gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt
     2221 tggggcatct ttcccggccg tggattggtg actgggtgtc gcgcaataaa aaatggattg
     2281 cgaaaactga ccagacgtcc attctgttgg tggtttatac agcgtttagc gaagccgtcg
     2341 ttaatggtat ctggcataaa gttggctggg gatcattgct gtttatcgtg gtggtcagct
     2401 gcgttcttct ggctatcgtg attgtagtta acgtctttat ggcacgccga ctgggcttca
     2461 ataaggcaga tgaaattact atcgtctttt gtggttcgaa aaagagtctg gcaaatggca
     2521 tcccgatggc aaacattctt ttccccacat cggtgatcgg tatgatggtg ctgcctctga
     2581 tgattttcca tcagatccaa ttgatggtct gtgcggtgct ggcgcgtcga tacaaacacc
     2641 agaccgaaca gttacaggcg cagcaggaaa gccgcgccaa taaagcttaa agaggacgct
     2701 tcaggggctg gaccagttgc gtcagcccct cagttttaat cagcagcgtg atttgcatta
     2761 gctcaccgag cttccctgct gggaactcat ctttgcgggc aaaccacaga agatactcct
     2821 ccggcaggtc gattaagcga cgccctttgt atttgccaaa cggcattatc gtattggcta
     2881 tttcaatcag ctgctctttt tccatctcag ctacccagca aacgcagcat ttccgcttcg
     2941 tcgataactt ctatgcccag ttcctgcgct ttcgccagtt tagaacctgc cgcttctcca
     3001 gctatcacca gatcggtttt cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc
     3061 agtcgagctt tagcgtcatc acgtgacatc tggcttaagc tgcccgtaag caccacggtt
     3121 ttaccagcaa acgggctgtc aatctcttcc gcgttaatca ccaccggcgc aggccagtga
     3181 acgccttccg ccaacagctc gctgatgaca ttgcggttgc tttcttcggc aaagaagttg
     3241 tgaacgtggg atgcaacgac aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa
     3301 gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg
     3361 ccgacttcac ggatgccaag tgcatagagg aagcgagcaa aggtggtttc tttcgctttt
     3421 tccagcgcat taaccacgtt ttgtgccgat tttggcccca tacgctccag tccggtaagt
     3481 ttgcctgcgg tgagtttgaa cagatccgcc ggagtgtgga catattcttt ttcaaccagc
     3541 tgatcgatga ttttgtcgcc cataccgtca acgtccatcg cacggcggga aacaaagtgt
     3601 ttcagcgact ctttacgctg cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct
     3661 tcaccttcca cacgctcaac gtcagaacca cataccggac aatgcgtcgg gaatacaacc
     3721 tcacgggtat cttccgggcg ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg
     3781 ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac caagacgttc gatttcatcc
     3841 gcattgtgta aggttgcgtt actcaccagc acgcctgcaa catggacagg ttccagacgc
     3901 gcaacaggcg taatcgcgcc agtacgccca acctgaaact cgacgtcacg cacaaaggtc
     3961 atctgttcct gcgccgggaa tttaaacgct accgcccagc gcggggcacg cgcgacaaag
     4021 ccaagctgct cctgctgtgt cagtgagttg accttaatca ccacgccgtc gatatcaaag
     4081 cccagcgtcg ggcggtcttc ttccactttg tgatagaacg ccagcacttc ttccgccgat
     4141 tcacaaagtg ttacccggtc gctgaccggc aaaccccact ttttaaattg cagtaaacgg
     4201 ccaagatgag tattcggcag ctcgccacct tccagaatac cgacgccata gcagaaaaaa
     4261 gtgagcggtc gcttcgctgt aatacgcgga tcaagctgac gcagtgaacc agcagccgca
     4321 ttacgtgggt tagcaaacac tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg
     4381 aaccccgcct gcggcaggaa cacttcacca cgcacttcca gacgcgccgg gatattctct
     4441 ccgtgtagct tcagcggaat ggcgcgaata gtacgcacat tagacgtaat atcttccccg
     4501 gtagtgccat cgccacgcgt cgcggcactg actaaaacgc cattttcata cagaatactg
     4561 acggcaagac catccagctt cagctcacag caccaggtga ctttctcgtt gcttttcaga
     4621 cggtcctgca cacgtttgtt gaaagcaaga aagctttctt catcaaaaac gttatccagc
     4681 gacagcattg gcacttcatg gcggatctgg ctgaaagccg ccagcggcgc agcacctaca
     4741 cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt
     4801 tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg gcgcatccat cacatgataa
     4861 agatattcat gatggcgaag cgtcgttcgc agttctgtca gttgttgttc gattgattcc
     4921 atatcgcacc atcaatgcta aaaacccccg acaagcgggg gttcgaagag gagttaattt
     4981 gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg
     5041 caatttctgc ggagtcatca tacggcgctg atcgtcaagc actacaccgc ccacttcatc
     5101 ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc tgcagctcgt caccgtaaga
     5161 cggtacctgc ataaagatag tgacacccgg agtagtgaaa tccttcattt caggatcaaa
     5221 ggttcccggt ttcaccatat tcgccaggct aaataacgcc gggccgctgc catccgggct
     5281 aagatgacga tggtaaatat tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt
     5341 aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc gcgacgttca tgataatcac
     5401 cgcttctttg cgcttcggtt tatccataac tggagccggt tccgctacag gctcaggctg
     5461 tggtgccgct acaggctctg caggctggaa agcctgttgt gccggttgcg gtgctgaatg
     5521 cactggctgc ggcgctgatg cgacctgtgg cgaaactggc tgctgcaacg gttgttcagg
     5581 ctgcggctga taggcaggct gctgcacggg ttgctgcacc ggctgcgctg gacgcggagc
     5641 atgttgcggc ggtacctgcg cttcaggcgg ctgctggacc ggttgacgcg gctgcgcaga
     5701 cgcataaggc ggttggtact ggtgttgcgg cgacggacga gcagcctcat gctcctgggc
     5761 gttagccggg gcatgattca cgcggtgaac acgaacctca ccaacgccct catcatcttc
     5821 gacatcctcg tcataagaat cgtcgtcacg ttttgacttc attcgtttta atggccgatc
     5881 gcggaacata gaagatcgtt ctttacggct ggtccagaaa ccatgtacca gtaaagcgat
     5941 tatggcgatc gcgccaacaa tgattaatat cagacgcaaa tcctgcatca ttatattctc
     6001 tgttgttcta acaccttgcc accacggcaa acatttactc actaagagta tttgccgatt
     6061 acctcaagtg caagtgcact attaactttc acagcacaaa gatagatgaa atcgtgcttt
     6121 ttgctgtttt ttcgaacata tcctaactgt ccattgcgca attacccggt aaaatacgca
     6181 gaattttcct gggattggtc aaaaggagct catcctgatt atggtttcat cattcacatc
     6241 tgccccacgc agcggttttt actattttgc gcaaggctgg aagctcgtct cgcaacctgg
     6301 gattcggcgt ttcgttattt taccgctgct ggtcaatatt ttgttgatgg ggggcgcatt
     6361 ctggtggctc tttacgcaac tcgatgtctg gatcccgact ctcatgagtt atgttccgga
     6421 ctggttacaa tggctgagct atcttttgtg gcctctggca gtcatctccg tgctgttagt
     6481 gtttggctat ttcttctcaa cgattgctaa ctggattgcc gctccgttta acggtttatt
     6541 ggctgaacaa ctggaagcac gattgactgg cgctacaccg ccagataccg ggattttcgg
     6601 tatcatgaaa gatgtgccgc gaatcatgaa acgcgaatgg caaaaatttg cctggtatct
     6661 gccgcgcgca attgtattgc taattcttta cttcatccct ggtattgggc aaaccgtcgc
     6721 gccggtactg tggttcctgt ttagcgcctg gatgttagcc atccagtatt gcgattaccc
     6781 cttcgataac cacaaagtgc cgtttaaaga gatgcgcacc gccctgcgca cacgcaaaat
     6841 caccaatatg cagtttggtg ctttaaccag cctgtttacg atgatccctc tgcttaatct
     6901 gttcatcatg cccgttgccg tttgtggtgc gacggcgatg tgggtcgatt gctatcgcga
     6961 taaacacgcg atgtggcggt aacaatctac cggttatttt gtaaaccgct tgtgtgaaac
     7021 aggggtggct tatgccgccc cttattccat cttgcatgtc attatttccc ttctgcatat
     7081 agatatgcta aatccttact tccgcatatt ctctgagcgg gtatgctacc tgttgtatcc
     7141 caatttcata cagttaagga caggccatga gtaagatttt tgaagataac tcgctgacta
     7201 tcggtcacac gccgctggtt cgcctgaatc gcatcggtaa cggacgcatt ctggcgaagg
     7261 tggaatctcg taaccccagc ttcagcgtta agtgccgtat cggtgccaac atgatttggg
     7321 atgccgaaaa gcgcggcgtg ctgaaaccag gcgttgaact ggttgaacca accagcggta
     7381 ataccgggat tgcactggcc tatgtcgctg ccgctcgcgg ttacaaactt accctgacca
     7441 tgccagaaac catgagtatt gaacgccgca agctgctgaa agcgttaggt gcaaacctgg
     7501 tgctgaccga aggtgctaaa ggcatgaaag gcgcaatcca aaaagcagaa gaaattgtcg
     7561 ccagcaatcc agagaaatac ctgctgctgc aacaattcag caatccggca aaccctgaaa
     7621 ttcacgaaaa aaccaccggt ccggagatct gggaagatac cgacggtcag gttgatgtat
     7681 ttattgctgg cgttgggact ggcggtacgc tgactggcgt cagccgctac attaaaggca
     7741 ccaaaggcaa gaccgatctt atctctgtcg ccgttgagcc aaccgattct ccagttatcg
     7801 cccaggcact ggctggtgaa gagattaaac ctggcccgca taaaattcag ggtattggcg
     7861 caggttttat cccggctaac ctcgatctca agctggtcga taaagtcatt ggcatcacca
     7921 atgaagaagc catttctacc gcccgtcgtc tgatggaaga agaaggtatt cttgcaggta
     7981 tctcttctgg agcagctgtt gccgcggcgt tgaaactaca agaagatgaa agctttacca
     8041 acaagaatat tgtggttatt ctaccatcat cgggtgagcg ttatttaagc accgcattgt
     8101 ttgccgatct cttcactgag aaagaattgc aacagtaatg ccagcttgtt aaaaatgcgt
     8161 aaaaaagcac cttttcaggt gcttttttgt ggcctgcttc aaactttcgc ccctcctggc
     8221 attgattcag cctgtcggaa ctggtattta accagactaa ttattttgat gcgcgaaatt
     8281 aatcgttaca ggaaaagcca aagctgaatc gattttatga tttggttcaa ttcttccttt
     8341 agcggcataa tgtttaatga cgtacgaaac gtcagcggtc aacacccgcc agcaatggac
     8401 tgtattgcgc tcttcgtgcg tcgcgtctgt taaaaactgg cgctaacaat acaggctaaa
     8461 gtcgaaccgc caggctagac tttagttcca caacactaaa cctataagtt ggggaaatac
     8521 aatgttccag caagaagtta ccattaccgc tccgaacggt ctgcacaccc gccctgctgc
     8581 ccagtttgta aaagaagcta agggcttcac ttctgaaatt actgtgactt ccaacggcaa
     8641 aagcgccagc gcgaaaagcc tgtttaaact gcagactctg ggcctgactc aaggtaccgt
     8701 tgtgactatc tccgcagaag gcgaagacga gcagaaagcg gttgaacatc tggttaaact
     8761 gatggcggaa ctcgagtaat ttcccgggtt cttttaaaaa tcagtcacaa gtaaggtagg
     8821 gttatgattt caggcatttt agcatccccg ggtatcgctt tcggtaaagc tctgcttctg
     8881 aaagaagacg aaattgtcat tgaccggaaa aaaatttctg ccgaccaggt tgatcaggaa
     8941 gttgaacgtt ttctgagcgg tcgtgccaag gcatcagccc agctggaaac gatcaaaacg
     9001 aaagctggtg aaacgttcgg tgaagaaaaa gaagccatct ttgaagggca tattatgctg
     9061 ctcgaagatg aggagctgga gcaggaaatc atagccctga ttaaagataa gcacatgaca
     9121 actgacgcag ctgctcatga agttatcgaa ggtcaggctt ctgccctgga agagctggat
     9181 gatgaatacc tgaaagaacg tgcggctgac gtacgtgata tcggtaagcg cctgctgcgc
     9241 aacatcctgg gcctgaagat tatcgacctg agcgccattc aggatgaagt cattctggtt
     9301 gccgctgacc tgacgccgtc cgaaactgca cagctgaacc tgaagaaggt gctgggtttc
     9361 atcaccgacg cgggtggccg tacttcccac acctctatca tggcgcgttc tctggaactg
     9421 cctgctatcg tgggtaccgg tagcgtcacc tctcaggtga aaaatgacga ctatctgatt
     9481 ctggatgccg taaataatca ggtttacgtc aatccaacca acgaagttat tgataaaatg
     9541 cgcgctgttc aggagcaagt ggcttctgaa aaagcagagc ttgctaaact gaaagatctg
     9601 ccagctatta cgctggacgg tcaccaggta gaagtgtgcg ctaacattgg tacggttcgt
     9661 gacgttgaag gtgcagagcg taacggcgct gaaggcgttg gtctgtatcg tactgagttt
     9721 ctgttcatgg accgcgacgc gctgcccact gaagaagaac agtttgctgc ttacaaagca
     9781 gtggctgaag cgtgtggctc gcaggcggtt atcgttcgta ccatggacat cggcggcgac
     9841 aaagagctgc catacatgaa cttcccgaaa gaagagaacc cgttcctcgg ctggcgcgct
     9901 atccgtatcg cgatggatcg taaagagatc ctgcgcgatc agctccgcgc tatcctgcgt
     9961 gcctcagctt tcggtaaatt gcgcattatg ttcccgatga tcatctctgt tgaagaagta
    10021 cgtgcactgc gcaaagagat cgaaatctac aaacaggaac tgcgcgacga aggtaaagcg
    10081 tttgacgagt caattgaaat cggcgtaatg gtggaaacac cggctgccgc aacaattgca
    10141 cgtcatttag ccaaagaagt tgatttcttt agtatcggca ccaatgattt aacgcagtac
    10201 actctggcag ttgaccgtgg taatgatatg atttcacacc tttaccagcc aatgtcaccg
    10261 tccgtgctga acttgatcaa gcaagttatt gatgcttctc atgctgaagg caaatggact
    10321 ggcatgtgtg gtgagcttgc tggcgatgaa cgtgctacac ttctgttgct ggggatgggt
    10381 ctggacgaat tctctatgag cgccatttct atcccgcgca ttaagaagat tatccgtaac
    10441 acgaacttcg aagatgcgaa ggtgttagca gagcaggctc ttgctcaacc gacaacggac
    10501 gagttaatga cgctggttaa caagttcatt gaagaaaaaa caatctgcta atccacgaga
    10561 tgcggcccaa tttactgctt aggagaagat catgggtttg ttcgataaac tgaaatctct
    10621 ggtttccgac gacaagaagg ataccggaac tattgagatc attgctccgc tctctggcga
    10681 gatcgtcaat atcgaagacg tgccggatgt cgtttttgcg gaaaaaatcg ttggtgatgg
    10741 cattgctatc aaaccaactg gaaacaaaat ggtcgcgcct gtagacggca ccattggtaa
    10801 aatctttgaa accaaccacg cgttctctat cgaatctgat agcggcgttg aactgttcgt
    10861 ccacttcggt atcgacaccg ttgaactgaa aggcgaaggc ttcaagcgta ttgctgaaga
    10921 aggtcagcgc gtgaaagttg gcgatactgt cattgaattt gatctgccgc tgctggaaga
    10981 gaaagccaag tctaccctga ctccggttgt tatctccaac atggacgaaa tcaaagaact
    11041 gatcaaactg tccggtagcg taaccgtggg tgaaaccccg gttatccgca tcaagaagta
    11101 attcttgccg cagtgaaaaa tggcgcccat cggcgccatt ttttatgctt ccgccagcgg
    11161 cggcaaaatc aattcatcgc tctcatgctg ctgggtgtag cgcatcactt ccagtacgcg
    11221 caaccccgca cggtgcaccg catcgtttaa cgccttccct ttcagcaaac cactgataag
    11281 ctgagcacaa aacaggtcgc cagtcccttt caggtcggtt tttacccgtg aatgggaaat
    11341 gacattcacg ctgtcggcac tgaccaccac cacctgcatc tcctgatttt cttcattacc
    11401 ggaggcgctg gtaatcacca cccattttaa tgtgtctgaa agcagacttt ttgcggcagc
    11461 aatggcactg tcgagatcgc ggcaattttt accggtcagg atttccagct caaagatatt
    11521 gggggtaatt ccctgcgcca gcggcagtaa atattgtcga tacgcctcgg gaaggtcagg
    11581 tttgacataa attccgctat caatatcgcc aatcactgga tcgaccatga tcaataggtc
    11641 aggatgatct ttgcgtagcg cagtcagcca ctcggcaagg attttgattt gcgatgccgt
    11701 tcccatatag cccgtggtta cagcacgaag ttggcgcaac gcatcacgct cctgaagcgc
    11761 acgcaaatag ccgctaaacc attcgtccgg aatcgcacca ccgtagaaag tgtcataatg
    11821 cggtgtattg ctcagcaata ccgtcggcac ggcaaagaca ttcaggccgt tctgtttgat
    11881 agcaggcacg gcaatgctgt tgcctacgct gccgtacact acctgcgact gcacggcgac
    11941 gatatctgcc tgcaatgccc tgctcttatc gttaaacaac aacaaactac tcatttaatt
    12001 ttttctcctt gccgatgatc ctcatcgtat tccaaccgaa actttacctg attctggcag
    12061 tcaaatcggc tatcacaaaa caaggataag gtaattcaat gaagaaaatc atttgtctgg
    12121 tcattacgct gctaatgaca ctccccgttt acgcgaagtt aactgcccat gaagaagccc
    12181 gtatcaacgc catgctggag ggattagcac agaaaaagga tttgatattt gtgcgtaacg
    12241 gcgatgaaca cacctgtgat gaagcggttt cccacctgcg tctgaagctc ggcaataccc
    12301 gtaaccgcat tgacactgcc gaacaattta ttgataaggt tgcttcgtca tcatcgatta
    12361 ccgggaagcc gtatatcgtg aagatgcccg gtaagagcga tgaaaatgcg caaccttttt
    12421 tacatgcgct gattgcgcag acggataaaa cggtgtctgc gcaataatct taaatccccg
    12481 ccccctggct aaagtgttct tccccaaaca cgccggtgga gaggtagcga tcgccgcgat
    12541 cgcagatgat cgccacaacc accgcgccgg ggttagcttt tgccacgcgc agtgctccgg
    12601 caaccgcgcc gccggagcta acgccacaga atattccttc ccgcaccgcc agttcacgca
    12661 tggtgttttc tgcatcgcgc tgatgaatat ccagcacctc atccaccagc gaagcgttga
    12721 aaatccccgg cagatattcc gcaggccagc ggcgaatgcc gggaatgctg ctgccctctt
    12781 ccggttgcag gccgacaatg gtcaccggtt tggattgttc gcgcataaag cgtgacacgc
    12841 cggtgatggt gccggttgtt cccatgctgg agacaaaatg ggtaatgcgc ccgccagttt
    12901 gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg ggattattga
    12961 actgatcgag cagctttcct tcaccacgat tcgccatctc cagcgccaga tcgcgcgcac
    13021 cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca cgcatcgccg
    13081 cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag cctttaagcg
    13141 cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatc agcacgtcac
    13201 ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc gcacgatctt
    13261 tcaccgaacc tgccgggtta ttgccttcca gtttcaacca gacttcactg ccgttattcg
    13321 gccccattcg ctgcaacttc accagaggcg tattgccaat tgtttgttct aatgtactca
    13381 cgatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta tcaggcctac
    13441 aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg ggcgtttaac
    13501 attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata
    13561 cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg ctcgccacgt
    13621 tgcggggcgt cgtcgccgtg catcacgacc gtcagcgctt cgttgtacca ccccagcggc
    13681 tgcacgacta attgggtgta gtggcctttc gggctggctt ccagtacctg taccggcagc
    13741 ggcgaatcga ggctggtacg gcggctgata tccacttccc acgggcgcag gaagagatcc
    13801 accggcccct gatacgcagg tgtatagccc agcggccagc gatgtgcgcc aacatggaac
    13861 tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc gagcacaaag
    13921 cgggtcgctg gttcgcgcca tacctgatcc ggcgcatcag cctgctcgat attgccctgg
    13981 ctcatcacca ctacacgatc ggctacttcg gtcgcttctt cttggtcatg ggtcacgaat
    14041 acgctggtaa atttcagttc ttcatgcagt tgacgcaacc agcgacgcag ctctttacgc
    14101 acctgcgcat ccagcgcgcc aaacggttca tcaagcagca gaatttgcgg ttccacagcg
    14161 agcgcacgcg ccagcgccac gcgctgtttc tgcccgccgg aaagctgtgc cggataacga
    14221 tccgccagat gcgcaagctg caccatctcc agcaattttg tcactttcgc tttgatggct
    14281 gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc cgaaagcgat attgtcgaac
    14341 accgtcatat ggcggaacag cgcatagtgc tggaacacga aaccaacttt acgatcgcgc
    14401 gcatgcaggc ggctgacgtc ggtgccgtgg aagcgaatat gcccgctggt ttgatgctcc
    14461 agcccggcga taatgcgcag cagcgtggtt ttcccggaac cggacggccc cagcaacgcg
    14521 accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt gcgaccaaaa
    14581 gacttcttaa tattggcaat ctcaatgctc atgatgttcc tcctgctgcg cgcgtttttc
    14641 ctgattctcc agacgccact gcaacatact ctttaaaaac agggtgataa tcgccatcag
    14701 cgtcaacaac gcggcagcgg taaaggagcc gacggtgttg tagtcttgct ccagcaattc
    14761 aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca ccgacaccgc
    14821 accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccataaagca gcgcccagcg
    14881 gatgttcggc agcgtgacgc ggcgaaacat ctgccagcca gatgcgccca gcaaaatcgc
    14941 cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcac gcaccacaaa
    15001 cggacacgtc acgaagatgg tgaccagcac cattcccggc caggagaaca taatttgcag
    15061 attatgttca tccagccagc cgccgagcgg gccgttagag ccgtaaaaca gcagatacac
    15121 cagaccggca accaccggcg acacggcaaa cggaatgtcc agcagcgtca gcagcaactg
    15181 gcgtccgggg aagttaaagc gcgtcaccag ccatgccagc agaatgccga acaccaggtt
    15241 caccggcacg gcaatcagcg caatcatcac cgtcagccag atggcgtgca gcatgtccgg
    15301 atcggccaga ttctgtaaaa ctggcatcag ccctttactg aacgcctgca caaagatgta
    15361 aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa tcagaaacca
    15421 tttgccccag ttaatcgggc gcgcgtcata acgcttcaac tgtgtcactt ccgccattaa
    15481 tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa cagcagcagc
    15541 agggatgccg cgaggatcac cgaagcaatc gcgctcgccg ccgggtaatc aaactcctgt
    15601 aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc aggcgatgtt cccggcgata
    15661 aaaatcaccg cgccaaactc accaagacta cgagtaaacg acagcgccac gcccgccacc
    15721 agcgccggag aaagttccgg cagcaccact ttgcagaaac tctgccagcg cgttgcgcca
    15781 agcgtttctg ccgcttcttc atattccggg cctaactctt ccagcaccgg ctgcacggta
    15841 cgtaccacaa acggaatgct ggtaaaggcc atcgccaccg caatccccag ccaggtatag
    15901 gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac agaaaagagc
    15961 gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc catcagcgca
    16021 tcaagcagcg tgcggcccgg aaagcgataa cgggttaaga tccacgccat cagcaggccg
    16081 aaaacgccgt taaaaattga tgccacaaac gccgacagca gcgtcacttt gtaggccgcg
    16141 accacttgtg ggttggtgat cacctcccag tactgcgccc agctcatctg agccagctgc
    16201 atcaccagcg cggagagcgg cagcagcaaa ataaggcaca caaacagcag actggtgccg
    16261 aggcttaagg taaagcccgg cagcacgcgt cttgatgaaa ctgcaaacat taattacgcc
    16321 ccgccgctaa cagcttgtct aactcgccac cgctggtgaa atgggttttc atcacttccg
    16381 gccaggagcc aaatttgtct tccacgcgga acagctcggt ctgcgggaat ttatctttca
    16441 gtttgtccat gacttccggg ttatttacgc ggtagtaata gtcggtgatg atggtctgcg
    16501 cctgcgggct gtagagccag ttcaggtaag cttttgctgc tttttccgta ccgttggcct
    16561 gcacgttttt atcgacccac gccaccggga attccgccag aatattggtt ttcggaatca
    16621 ccacttcaaa gccttgcgct tcatactgtt tgcgaatgtt gttcacttcc gactcgaagc
    16681 tgatcagcac atcgcccagg ccgcgctcgg caaaagtggt ggtcgcgcca cgaccgccag
    16741 tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt ttggctttgt
    16801 cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtgtaa cgtgcgttac
    16861 ccgacgtttt cgggttcggg aaaatcagct ttacgtcgga gcgcaccaga tcgttccagt
    16921 cgtggatatt cttcgggtta cccttacgca ccaggaagcc catggtggag tagaacggcg
    16981 agctgttatt cggcaggcgc gtttgccagt cggccgggat cagcttgcct ttgtcgtgca
    17041 ggatttgtac gtcagtcacc tggttataag tgacaacgtc ggcttttaag ccctgcaaaa
    17101 tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt ttgtcgccgc
    17161 cgttatcttt ggcccattgt tgctcaaacg gcggattcag ggcggcaaac agctcgcggg
    17221 agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc agcagcagag
    17281 aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg cacccttata
    17341 aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga gtaacggttt
    17401 tatataccgt ttggtgattt ggaagttgaa aagggaataa gaaccgggtt caagacgaaa
    17461 tcctccccac aaatgcaggg aggaaacaga ggtgaatcag atgccgacgc taaccgtctc
    17521 cggcagtgtg ctgccgccat caatcacatt ctgtgtaccg gttaaataac ttgattcatc
    17581 cgatgcgagg aaggctgcca gttcgccgac ttccagcgga tcggcgaggc gacgcatcgg
    17641 gattgctttt gccatttcag tcagcaccga ttctggatct tccgggttcg actggcgggc
    17701 aatgctttcc gccattggcg tgcgtacgta tcccgggcag atggcgttaa cgcgaatacc
    17761 agactgcgcg tactccaccg ccagcgattt agtcaggcca acaatcgccg ctttcgttaa
    17821 ggcatacgcc gtttcacccg gatcggccac catatcgcca gtgactgaag acatcatcac
    17881 aatgcgacca tctttgcggg caatcatctc cggcagcacc gccttcgtga cgttccatac
    17941 gcctttaata ttgatatcaa tatggaaatc gcgatcttca tcgctcatat cgaggaaact
    18001 gcccagacga caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt
    18061 cgcgcgcttg atagctgcgg cgaccgacgc cgggtcacgc acatcggcga caaccgccgt
    18121 acagcgatga ccacgaccac acagttcgtc cgccagcttt tcgatctcag gggagatatc
    18181 cagcaagatt aggtttgcgc catgacgcgc aaaagttctg gcaattcctt cgccaattcc
    18241 ctgcaatgcg cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc
    18301 cttgttaata cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt
    18361 gctgcggatc agctcactaa ttcaggaaaa tctcaatcca gtcgctgctt tatgcatcat
    18421 ttcaggcgtt gcgttagctc actgctgcgc tcaatcattt tcaatgactc cacatcattg
    18481 agttgcacaa ggccaacaaa cagtaaatcg gtaacagaat tttgcgccgt gcgggtggac
    18541 atcgacgagc tgcgccattc ggtttcgcca gaaacggtat cgagggtgta atgcgccagc
    18601 cgccgcagcg gggaatcggt taacgaggta atggcaatca ccgttgcccc ctgctttcgt
    18661 gctgcctcgg cgcacaagac aatctctttt ttactgccgc tgtaagagat agcgatttgt
    18721 acatcgcctt ttttcagcgc ctgggaaacg gtggcttgta catgcgtgtc agcttcacag
    18781 gccacacgat aaccaatttt catcagtttg aatgaaagat cgcgccccac cagcgccgac
    18841 ccacccaggc ccgttatctg gataaacggt gctttactga tgacctcaat gattttttgc
    18901 aaccgcgcgt agtcgaacaa cgcacaggtt tgctccagcg ccagctcttt ttcgcgattc
    18961 aatttgcggg caatcacttc cagcgaatca tcactggtaa tcgaactgtg caggtgcagc
    19021 gccgtggcat tcgttttctc ccggctagca ctgtattcgc caattaacgc catacgcaat
    19081 tcagtaaatc cttgcgcacc gagcttctgg gcaaacttca caatactcga ctggctgatg
    19141 ccgagctgtt tcgccatctt gcgcgaagag acggatttca gctcactcac gttggcccgc
    19201 agaaagtcag caattttttg ctcgttcccg gtaaattcac tttccgcatt gcgtattttc
    19261 gtcaggtaca acatcttcca ctctcctggg ctggcagcaa aaattccgct taagttctta
    19321 tgacgctctt cacactctgc gagtgtagac tcaatgattc ctttaagtct gtcaattcgg
    19381 gatatatgat tccatacatc tgcaattatt tgatcgtaaa tagtaaggtc accaccgatg
    19441 caacttgaaa agatgattac cgaaggctcg aacgccgcct cggctgaaat tgaccgcgta
    19501 tcaacgctgg aaatgtgccg gattatcaac gatgaagata aaaccgtacc actcgccgtt
    19561 gagcgcgtac tgccggatat cgccgcggcg atcgatgtta tccacgccca ggtaagcggc
    19621 ggcgggcgtc tgatatatct cggtgcggga acatccggtc gtctggggat tctggatgcc
    19681 agcgaatgtc cgcccaccta cggcgttaaa ccgggtctgg tggttggttt gattgctgga
    19741 ggcgaatatg ccattcagca cgcggtggaa ggcgctgaag atagccggga aggtggtgtt
    19801 aacgatctga aaaacatcgg cttaaccgcg caggatgtgg tggttggcat tgcggccagt
    19861 ggtcgcacgc cgtatgtaat tgccggactg gaatacgcac gccagctcgg ctgccgcaca
    19921 gtgggaattt cctgtaatcc cgggagcgcc gtttcaacca ccgctgagtt tgccattacg
    19981 ccagttgttg gggccgaagt ggtcacaggt tcttcgcgaa tgaaagcagg tacggcgcag
    20041 aaactggtgc tcaatatgct ctccaccggg ctgatgatta aatccggcaa agtgttcggc
    20101 aacctgatgg tcgatgtggt cgccaccaac gaaaaattgc atgtgcgcca ggtcaatatc
    20161 gtcaaaaacg ctaccggatg tagcgcagag caagcggaag cggcattggt tgcctgcgag
    20221 cgcaactgta aaacggccat tgtgatggtg ctgaaaaatc tcgatgccgc agaagctaaa
    20281 aaacgtcttg atcaacacgg cggctttatt cgtcaggttt tagacaagga ataacccatg
    20341 gccaaagaga tcagcagtga acttctgaac accattctta cccgtgtcgg cggaccggga
    20401 aatatcgcca gttgtggtaa ctgtatgacg cgcctgcgtc tgggtgtaca tgacagttca
    20461 ctggttgatc ccaatatcaa aacgctggaa ggcgtgaagg gcgtcatttt gaccagcgat
    20521 caggtgcagg tggtttttgg gccgggcaaa gcgcatcgtg ccgcaaaagc catgagcgag
    20581 ctgctgggag aagcaccggt acaggatgcc gcagaaatcg ccgcccagaa caaacgtcag
    20641 ttaaaagcca aacaaacctc cggcgtgcaa caatttctcg ccaaattcgc caccatcttc
    20701 acgccgctga tccccggttt tattgccgcc ggtctgctgc tggggatagc gacgttaatt
    20761 gccacggtga tgcacgttcc ggcagatgct cagggcacgc tacccgacgc gctgaatttt
    20821 atgaaggtgt tcagcaaagg tttgttcacc ttcctggtga ttctggtggg ctataacgcc
    20881 gcccaggcat ttggcggcac gggcgtaaat ggcgcgatta tcgccgcgct ctttttgctc
    20941 ggttataacc caactgcaac caccggttac tacgccggtt ttcacgattt ctttggtctg
    21001 cccatcgatc cgcgcggcaa tattatcggc gtgttgattg ccgcgtgggc ctgcgcccgc
    21061 atcgaaggca tggtgcgccg ctttatgccg gacgatctcg acatgctgtt gacctcgtta
    21121 atcaccctgc tgatcaccgc tacgctggcc tacctgatca ttatgccgct gggcggctgg
    21181 ctgtttgaag gcatgtcgtg gctgtttatg cacctgaaca gtaatccgct cggttgtgcg
    21241 gttttagccg ggctgttcct gatcgccgtg gtgtttggcg tgcatcaggg ctttattcct
    21301 gtttacctcg cgttaatgga cagtcaggga ttcaacagct tatttcccat cctttcgatg
    21361 gcaggcgcgg gccaggtggg cgcggcgctg gcactctact ggcgggcgca accgcacagt
    21421 gcattacgca gtcaggtacg cggggcgatt attcccggcc tgctgggcgt tggcgaaccg
    21481 ttgatttatg gcgtcaccct gccccgcatg aaaccgttta ttaccgcctg tttaggcggc
    21541 gcggcgggcg gtttgtttat cggattgata gcctggtggg gtctgccgat gggcttaaac
    21601 agcgcctttg ggccgtctgg tctggtggcg ctgccgctga tgacttccgc acaaggcatc
    21661 cttccggcaa tggcggttta tgcaggcggg attctggtgg catgggtttg cgggtttatt
    21721 ttcaccacgc tctttggctg ccgtaacgtc aatctggact gataatatga aacggacaat
    21781 gctctatctt tctctgctgg ctgtcagctg tagcgtcagc gccgcaaaat accctgttct
    21841 gacagaaagt tcgccagaga aagcagggtt caacgtcgaa cggcttaacc agatggatcg
    21901 ctggataagc cagcaggtgg atgccggtta tcccggcgta aacctgctga ttattaaaga
    21961 taatcagatt gtgtatcgca aagcctgggg agcggcgaaa aagtacgatg gcagcgtatt
    22021 gatggaacag cctgtcaaag ccaccaccgg gacgctgtat gacctggctt ccaataccaa
    22081 gatgtacgcc actaactttg ctctgcaaaa gctaatgagc gaaggcaagc tgcatcctga
    22141 cgatcggatt gcgaaatata ttccgggatt tgccgatagt ccaaatgaca ccatcaaagg
    22201 caaaaacacg ctgcggattt ctgacctgct gcatcacagc ggcggtttcc cggcggaccc
    22261 acaatatccg aataaagcgg tcgcgggcgc gttatattcc caggataaag gccagacgct
    22321 ggagatgatc aagcgcacgc cgctggagta tcagcccggc agcaagcata tctacagcga
    22381 tgtcgattat atgctgcttg gatttatcgt cgagtcggtt accggtcagc cacttgaccg
    22441 ctatgttgaa gagtcgattt atcgcccgct cggcctgacg catacggtgt ttaatccgct
    22501 actgaaaggc tttaaaccgc aacaaattgc cgccacggaa ctcaacggca atacccgcga
    22561 tggcgtgatc catttcccga atatccgcac ctccactctc tggggtcagg tgcacgatga
    22621 aaaagccttt tattcgatgg gcggcgtttc cgggcatgca gggttgtttt ccaataccgg
    22681 cgatattgcg gtgttaatgc aaacgatgct gaacggcggc ggctatggcg atgtgcagtt
    22741 gttcaatgca gaaacggtga agatgttcac caccagctct aaggaagatg ccacttttgg
    22801 ccttggctgg cgcgtgaatg gtaatgccac catgacgccg acgtttggca cgctggcaag
    22861 cccgcaaacc tacggtcaca ctggctggac cgggacggtg accgttatcg atccggtgaa
    22921 tcatatggcg attgtgatgt taagcaacaa gccacattcg ccagttgccg atccacaaaa
    22981 aaatcccaat atgttcgaaa gcggtcagct accgattgca acttatggtt gggtagttga
    23041 tcaggtgtat gcggcgttaa agcagtaata aaaaggccag tcagttgtga tactggctgg
    23101 ccttaaatca tcgaatcaga aacgattaca acgccatcaa cttgtccagc gacggcgcga
    23161 agtaatagcc gccagtgacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    23221 tatcgccaaa catgctcagc agttgctgct caatgttata cagacgcgcg cagtaggcgc
    23281 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    23341 tcagcccttt gccatcttct ttcagatcaa cgcgggtcag gtgagaggtt tccggacgct
    23401 cgtcgccgtc gatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    23461 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    23521 tgccgcccgc atccacgccg tctttgataa ccgccacttc acggcgcgtc tcttcacccg
    23581 ccgggttttc agtaccgtca acgaagccgc tcaggtcacg ctcttcaacc caacggaagc
    23641 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    23701 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    23761 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttcggcc ccaacgccgc
    23821 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    23881 ggaatttcgc ttcaaaagtt gccagtttat cggcaaatgt tttactggcc gcacgcaggg
    23941 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    24001 aaatgccact ctgaacctga gacattattc ctcctg
//
LOCUS       DARNVM010000066        23525 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.066, whole genome shotgun sequence.
ACCESSION   DARNVM010000066 DARNVM010000000
VERSION     DARNVM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.066"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1619
                     /locus_tag="SI621_002354"
     CDS             <1..1619
                     /locus_tag="SI621_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEI4065066.1"
                     /translation="IISVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMSGGAI
                     VMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIATAAQ
                     TLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAEDLSKN
                     GLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEVAVRE
                     KNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRILAER
                     QIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEA
                     RANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAA
                     EMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQSLPAVIEK
                     SVEPMKSIDGIKIIQVDGLNRGGAAGNAASGSVSGGNLAEQALSAALSYRTQAPLIDS
                     LLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1858..1917
                     /locus_tag="SI621_002355"
     CDS             1858..1917
                     /locus_tag="SI621_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEI4065067.1"
                     /translation="MMKLVIILIVLLLVSFATY"
     gene            2234..2293
                     /gene="ibsE"
                     /locus_tag="SI621_002356"
     CDS             2234..2293
                     /gene="ibsE"
                     /locus_tag="SI621_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HEI4065068.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2413..3846)
                     /gene="hldE"
                     /locus_tag="SI621_002357"
     CDS             complement(2413..3846)
                     /gene="hldE"
                     /locus_tag="SI621_002357"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HEI4065069.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3894..6734)
                     /gene="glnE"
                     /locus_tag="SI621_002358"
     CDS             complement(3894..6734)
                     /gene="glnE"
                     /locus_tag="SI621_002358"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEI4065070.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWQQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDCKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTAMDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6757..8058)
                     /gene="ygiF"
                     /locus_tag="SI621_002359"
     CDS             complement(6757..8058)
                     /gene="ygiF"
                     /locus_tag="SI621_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HEI4065071.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDINSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8300..8920
                     /locus_tag="SI621_002360"
     CDS             8300..8920
                     /locus_tag="SI621_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEI4065072.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8984..10222
                     /gene="cca"
                     /locus_tag="SI621_002361"
     CDS             8984..10222
                     /gene="cca"
                     /locus_tag="SI621_002361"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HEI4065073.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10303..11124)
                     /gene="bacA"
                     /locus_tag="SI621_002362"
     CDS             complement(10303..11124)
                     /gene="bacA"
                     /locus_tag="SI621_002362"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEI4065074.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFILAAAVYVVFF"
     gene            complement(11215..11583)
                     /gene="folB"
                     /locus_tag="SI621_002363"
     CDS             complement(11215..11583)
                     /gene="folB"
                     /locus_tag="SI621_002363"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HEI4065075.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11688..12305
                     /gene="plsY"
                     /locus_tag="SI621_002364"
     CDS             11688..12305
                     /gene="plsY"
                     /locus_tag="SI621_002364"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEI4065076.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12318..13250)
                     /gene="ttdR"
                     /locus_tag="SI621_002365"
                     /gene_synonym="dan"
     CDS             complement(12318..13250)
                     /gene="ttdR"
                     /locus_tag="SI621_002365"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HEI4065077.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13457..14368
                     /gene="ttdA"
                     /locus_tag="SI621_002366"
     CDS             13457..14368
                     /gene="ttdA"
                     /locus_tag="SI621_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HEI4065078.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIHDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELEVRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            14365..14970
                     /gene="ttdB"
                     /locus_tag="SI621_002367"
     CDS             14365..14970
                     /gene="ttdB"
                     /locus_tag="SI621_002367"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HEI4065079.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15019..16482
                     /gene="ttdT"
                     /locus_tag="SI621_002368"
     CDS             15019..16482
                     /gene="ttdT"
                     /locus_tag="SI621_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HEI4065080.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLNGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTSYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(16525..17538)
                     /gene="tsaD"
                     /locus_tag="SI621_002369"
     CDS             complement(16525..17538)
                     /gene="tsaD"
                     /locus_tag="SI621_002369"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEI4065081.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17776..17991
                     /gene="rpsU"
                     /locus_tag="SI621_002370"
     CDS             17776..17991
                     /gene="rpsU"
                     /locus_tag="SI621_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEI4065082.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18102..19847
                     /gene="dnaG"
                     /locus_tag="SI621_002371"
     CDS             18102..19847
                     /gene="dnaG"
                     /locus_tag="SI621_002371"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEI4065083.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20042..21883
                     /gene="rpoD"
                     /locus_tag="SI621_002372"
     CDS             20042..21883
                     /gene="rpoD"
                     /locus_tag="SI621_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEI4065084.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21962..22468)
                     /gene="mug"
                     /locus_tag="SI621_002373"
     CDS             complement(21962..22468)
                     /gene="mug"
                     /locus_tag="SI621_002373"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEI4065085.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22593..22668
                     /locus_tag="SI621_002374"
     tRNA            22593..22668
                     /locus_tag="SI621_002374"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22626..22628,aa:Ile,seq:cat)
     gene            complement(22722..23486)
                     /gene="nfeF"
                     /locus_tag="SI621_002375"
     CDS             complement(22722..23486)
                     /gene="nfeF"
                     /locus_tag="SI621_002375"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HEI4065086.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLHVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRCRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5667 a         6475 c         6030 g         5353 t
ORIGIN      
        1 cgattatttc cgtatgcatt ctgtttatta ttggaattat tttcgccagg ctctatcgtc
       61 gcgcttcggc agagcaagct tttgttcgta ctggtttagg tgggcaaaaa gtggtaatga
      121 gcggtggcgc aatcgtgatg ccgatctttc atgaaataat ccccatcaat atgaatactc
      181 tgaagctgga agtcagccgc tcaaccattg atagcctgat tacgaaagat cgtatgcgcg
      241 tcgatgtcgt tgtcgctttc tttgtgcggg taaaaccttc agtagaaggt attgccaccg
      301 ccgcccagac gctggggcaa cgcaccctgt cgcctgaaga cttacgtatg ttggttgaag
      361 ataaatttgt cgatgccctc cgtgcaacag ctgcacaaat gaccatgcat gagttacagg
      421 atacccgcga gaacttcgtg cagggcgtac aaaataccgt tgctgaagat ctgtcgaaaa
      481 acggcctgga actggaaagc gtttcactta ccaactttaa ccagacgtcg aaagaacatt
      541 tcaatcctaa caacgccttt gacgccgaag gtttaaccaa gctgacgcag gagacggagc
      601 gccgtcgtcg cgaacgtaac gaagttgaac aggatgtaga agttgcggtg cgtgagaaaa
      661 accgtgatgc actttcgcgg aagctggaga ttgaacaaca agaagcgttt atgacgcttg
      721 agcaggagca gcaggttaaa acccgtaccg ctgagcagaa tgcgaaaatt gcggcttttg
      781 aggccgagcg tcgtcgggaa gcagagcaga cgcgaattct ggctgaacga cagattcagg
      841 aaacggaaat cgaccgcgaa caggccgtcc gttccagaaa agttgaagct gaacgtgaag
      901 ttcgcattaa agagatcgaa caacagcagg tcaccgaaat cgccaaccag acgaaatcga
      961 tcgctattgc cgccaaatcg gaacagcagt cacaagcgga agcgcgtgcc aaccttgcac
     1021 ttgcagaagc ggtaagcgcc cagcaaaacg tagaaacaac tcgccagacc gcagaagccg
     1081 atcgtgctaa acaagttgcc ctaatcgctg ccgcgcagga tgcagaaacc aaagcggttg
     1141 aactgaccgt gcgggcgaaa gcagagaaag aagccgcaga gatgcaggcg gcggcaatcg
     1201 ttgagttagc cgaagctacg cgtaaaaagg gtctggcgga agcagaagcg caacgtgcgc
     1261 tgaacgacgc tatcaacgta ctttctgatg aacaaaccag ccttaaattc aaactggcgc
     1321 tattacagtc gttacctgca gtaatagaga aatccgttga gccgatgaag tctatcgacg
     1381 gcatcaagat tattcaggtc gatggattaa accgaggtgg tgctgcgggg aatgcggcat
     1441 caggcagcgt tagtggcgga aacctggcag aacaggcatt atcagccgca ctctcttacc
     1501 gcacacaggc accactgatt gactccttgc tcaatgaaat tggcgtttca ggcggctcac
     1561 tggcggcatt gacttcaccc ttaacctcaa caactcccgt cgcagaaaac gtagaataaa
     1621 ttttcaacta tgaacatccc tggcccaaaa ggccggggat tttttatatc tgcgttccgc
     1681 aaaaaactgc aaatgctcag gtcgttgcag ccttttgcgt gaccgctcgg ggaaggcaaa
     1741 actgcctctg ggaaagcatt gcgcggggtc tgacgctcat caacaatcgg gaagtcagca
     1801 aagggctgaa acgggaaagc ccctcccgag gaaggggcct tacagaagga aagggttatg
     1861 atgaagctcg tcatcatact gattgtgttg ttacttgtaa gtttcgccac ttattaacag
     1921 ccaatcagag gggggagaaa tcctccctca cccttgttcc tttactttag gttgaaaaaa
     1981 aatcagcgtc aagaaggttt cattaacaca tatccctggc ccaaaaagcc ggggattttt
     2041 tatatctgcg ttccgctaaa aggtgcaaat gatcaggctg ttgcagcgtt ttgcgtgacc
     2101 actcggggaa ggcaaaactg cctcagggaa agcgttgcgc ggggtccggc gctcatcaac
     2161 aatcggggag tcagcaaggg gctgaaacgg gaaagcccct cccgaagaag gggccttaca
     2221 gaaggaaagg gttatgatga agttcgtcat catactggtt gtgttgttac tgttaagttt
     2281 cccgacttac taacaactca tcagaggggg gagaaatcct cccttaccct tgttccttta
     2341 ctctaggttg aaaaaacaac agcgtcaata ggcctgccag gtacgaagcg agatctgtga
     2401 aacgctttcc ggttagcctt ttttatcctg ttggatcttc ttgatgatgt tggtcgtcga
     2461 gcaaccgtct tcaaagttga gcaccagcac ttcgccgccg ttggcccaga cttctttact
     2521 cccggcaatc tcttctggtt tatagtcgcc gcctttcacc agcaggtctg gcaagatccc
     2581 ggcgatcaag cgctgcggcg tgtcttcttc aaacgacacc acccagtcga ccgcttccag
     2641 tgcacccagc acaatcatac gctgttcgag agggttaacg gggcgggaat cccctttcag
     2701 ccgtttggtg gaggcatcgc tgttcacagc gacaatcagg cggtcaccca gtttgcgggc
     2761 atttgccaga taggagacgt gcccggcatg gagaatgtcg aagacgccgt tggtcatcac
     2821 cactttttca ccgcgtttac gcgctgccgc tacagcaagc ttcagttcct cttcggtcat
     2881 cacgccaaag ccggtatctg cacgtccacg tactgcgttt tccagctcga tcggcgaaac
     2941 cgtggatgtc cccagtttgc cgaccaccac accagccgcc gcattggcaa agaagcaggc
     3001 ttcttccagc gaattacccg ctgccagcgt tgccgccagg acaccaatca ccgtgtcgcc
     3061 cgcaccggta acgtcataca cttcctgcgc ctgggttggc atatgcagcg gcgctttacc
     3121 cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg agagttcgta
     3181 atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac atttaccgac
     3241 aacagcttca aattccgaga gattcggcgt taacagcgta gcgccgcggt agcgctcaaa
     3301 atcggtacct tttggatcaa tcagcaccgg aacacccgct ttacgcgcca gttggatcat
     3361 ctgctgtacg cttgccagcg cacctttggc gtagtcagaa agcaccagcg cgccaatcga
     3421 actcagcgcc tgattaatcc gttcgtgcag cggttgcgga tcaacacctt cgaaaccttc
     3481 ttcaaaatcc agacggatca gctgttggtt gcgggaaagt acccgtaatt tggtgatggt
     3541 cggatgcgtc ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca gagatttact
     3601 cagcgcgcgc gctgcatcgt caatgcccgt caacccgacc aggcgtgcat tagcaccgag
     3661 agaagcgata ttcatcgcca cgttagccgc gccgcccgga cgttcttcga tggtatttac
     3721 tttaaccacg ggcaccggcg cttccggcga gatacggctg gtgggaccgt accagtaacg
     3781 atccagcatc acatcaccaa ccaccatcac tcctgcacgt tcaaactctg gcagcgttac
     3841 tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat acttcattct
     3901 tccaccagcc acttctgcca gcttgcccgc accagttcac gctctgcggt gaagcaatcc
     3961 tccgacacat ggcccggcaa ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc
     4021 gtgtaagcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt ttgcgccagt
     4081 agttccagaa tgcgcacgtt gtctgaccag cgcgttaact tcggtttttc atgagcgtag
     4141 cgcaacacca gatattgagt aataaattcg atatcggtaa ttccgccttc atcggctttg
     4201 atatcaaagc gatcgcgatg tttattgccg agatgggcac gcattttctc gcgcatttcc
     4261 cgcacttctg tttgcagggt tttgccttcg cgcggcagcg tcataatctc ccggcgtact
     4321 gcgtcaaagt gcgcggtgag ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc
     4381 tgatgttccc acgtccaggc ctcgtttttc tgataatcgg caaacgcttc tgcggatgtc
     4441 accagcattc ccgccgcccc ggacggacgc aaccgtgcat ccacctcata caaaatgccg
     4501 gaagaggttc gcgtactgaa cagatgcata atgcgttgcg ccagacgcag ataaaactgt
     4561 cgcccgtcga tttcccgctc gccgtcagtc atcgcatcca tcgggcaatc atggaggaag
     4621 atcaggtcaa gatcggaact gtagcctaac tcccagccgc ccagcttgcc gtagccgacc
     4681 accgcaaaac cacgcccttc gcgttcgttt aggtgatttg gcttaccgta gcgggcaacc
     4741 atttgaaccc acgcctgctg aacgacggca tctatcatag cctccgccag ccaggttaag
     4801 tgatcgctca ctttcattac cggtagcgta ccggcgatat ccgctgccgc aatacgtaac
     4861 agttgcgcct gtttgaactg acgcagcgcc tcaagctgtt gttcttcgtc atcttccggc
     4921 acgcgcagca aatactggcg cagttcatca cggtaggcat ccatcgccgt cggctggtag
     4981 agagtgtttg gatcgagcag ttcgtccagc aataatggat aacgcgctag ctggctggca
     5041 atcatcggcg atgcggcaca cagagaaatc aaatgtttaa gcgccgcggg gaattcactg
     5101 agcaattcta aataggtagt gcgggtaaca attcccacca gcaaggcggt aatgcgcgac
     5161 agcgtgacag cagcgtcttc acgcgcacag acatcactta gcagatgcgg catcagatgg
     5221 tcgagcacct gacgcccgcg cggcccgatg gtacgcttat ccagctcttt gcgaaaatcg
     5281 gcaatcagcg tcagcacctg tttgcaatca tcttcgctaa gatgcgccag cactggcgta
     5341 gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga cagcgactct
     5401 tcctgagttt cgctttcgtc gtcgccaatc aattcattaa acacccggcg cacattggtc
     5461 atatgtgcgg tcagcgcccc agtcagttgc tgccagtcag caaagtccat cgcccacgcc
     5521 agccgcgcac ggttaagctc atcagaagga agcgtctggg tttgttcgtc gttaatgctt
     5581 tgcagcaggt tttccagacg ccgcaggaac agatacgcca ctcgcaattg ttcagcatcg
     5641 ttttcagaaa gcagatgcag cgcggcaatg acgctgagcg ttggcagtaa agagcgcgac
     5701 tgcagcgacg gttcgcgccc accgcgaatg agctggaaca cctgaacgat aaattcaatt
     5761 tcgcgaatgc cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg acgcacttca
     5821 cgggcaatca tccctttcat gttgcgcagc gactgaatca cgctgaaatc gatgtaacga
     5881 cggaaaacaa acgggcgcag catcgcacgc aactcgttag catagacgcc ttcgctatcg
     5941 cccataatcc gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa
     6001 taatcctcca acgcggcaaa gctcagcacc agcgggccac tttcgccaaa cggacgcagc
     6061 cgcatatcca cgcgatagac gaagccatcc tgcgttggtt gatccaacac tttaatcagc
     6121 cgctgcccca tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc gccctgcgta
     6181 caaccatgtt ccggccaggc aaaaatcaga tcgatatctg aggagaaatt cagttcccca
     6241 ccgcccagct tacccatgcc taaaatcagc agcggttgcg cttcgccctg cgcattgcac
     6301 ggcgttcccc actcgcggca gcaagcgtca tacagccagt cacgcgcggc gacaatcagc
     6361 gtctccgcca gatggctgag ctgctgcaat atgctctctt cagtaaccaa agccagcgtt
     6421 tgcgcccagg cgatgcgcac cataatgcgc cgccggaata gccgcagctc gcgcattaac
     6481 ccggcttcgt cactcacatt gctgagcgcc tcctgcaacc atgccgcgta atgctgccat
     6541 tcgtcggcct gcggcgattg gctttccagt tccgtcagcc actctggatg cgcaatcacg
     6601 ctgtcctgca caaaatcact gaaagtaagt actgacttcg cctgtgcgct aagtgatttc
     6661 tcggctaaag gctctggcag ccgctcaaca acggtctgcc agtactgctg taacggtgaa
     6721 gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc tgtgcaacca
     6781 gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcaatgc gttgcccggt
     6841 cgcaatagcg tgacgcagcc cctgccagtt ctccagccag gcttgcgcga cgacaggatc
     6901 atagtaaccc gccagcaaca gtattgagtt aatatcacgc gtcagacgtg gcaactggtc
     6961 acggtagcga tcgcccaacg gctggcagaa aacgcttttc agttcagcgg catggcggga
     7021 aagatggata tcggcaaagc gtttgaagga atcgctcatt ttactctgcg ctttggcatc
     7081 taaaaacggc tgccaggctt tgcttaccag ccattcggtc aacgccagct tcgccattgc
     7141 cgttttggta gagtagaccg ccgtcacggc agaaaccgca gaagcgatgg tcgcttcgca
     7201 ttgagtcagc aaatcacgta agtgagtgct cgctttgcgc ggcacaatac caccgaacag
     7261 catcagtgta tgacggacca gaccaatggc tgccagcacc tgttctttcg ccgcatcgtt
     7321 gccgcgtacc cacagttctt catgatactg ccattgcgct aacgccagct cgagcgccgc
     7381 ttccagcccc tgttccacat cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat
     7441 ttcacgcgcc ggattgccct gcgccagatg atagccacgc gccgctttgc tcaggctgcc
     7501 ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc
     7561 gccgctaagc agttccagtt ccagctcaca gataggttca gcaaattccc ccgctttcac
     7621 ctccccctga tcgagagcga tttcaattcg gctgtcatcg accgccacca gccatttttc
     7681 gcgataaaaa tcggtgctga acagcggttg tacgcgggaa gcgagatcgg cgggcaattc
     7741 gccgttcggc cagacttccg tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc
     7801 cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt
     7861 catctcatag cgaccgtttt cgccacgaat acgtaagccc atatcgtgcc cacgcagcca
     7921 gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt gctcgccgcc
     7981 cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa taaactttaa
     8041 ttcgatttcc tgagccatgg cctttactta tcggttatgt cacataagtg acgatgaacg
     8101 gcgaacttaa tgcgatcttt tttgtcagta gatagtattt tgcgccaaat tgccatgcaa
     8161 cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac agaggattcc
     8221 gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt ccactttcca
     8281 tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt tactcgcact
     8341 tagcgccact gccgtctcac acgctgaaga aacgcgctat gtttctgatg aactgaatac
     8401 ctgggtccgt agcggtccgg gagatcatta tcgcctcgtg ggcacggtta acgccggcga
     8461 ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga aagacagctc
     8521 tggccgtacc gcctggatcc cgttgaaaca acttagcact gagccaagtc tgcgctcccg
     8581 tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca atatcgataa
     8641 cacctggaat cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat
     8701 caacgggtta aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa
     8761 ggtcgatgcc gccagcgtac agctggatga caaacagcgc accatcatca tgcaatggtt
     8821 tatgtatggc ggcggcgtgc tggggcttgg cttgctgctc ggtctggtac tgccgcacct
     8881 gatcccaagc cgcaaacgta aagatcgctg gatgaactaa atcgccttct tcgttgcact
     8941 gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga tttatctggt
     9001 cggtggtgct gttcgggatg cattgttagg gctaccggtc aaagacagag attgggtggt
     9061 ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag gccgcgattt
     9121 tcctgttttt ctgcatccgc aaacgcatga agagtatgcg ctggcacgta ctgaacggaa
     9181 atccggttcc ggttacaccg gttttacctg ctatgccgca ccggatgtca cgctggaaga
     9241 tgatcttaag cgtcgcgatc tgaccattaa tgcgctggcc caggacgata acggcgagat
     9301 tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc
     9361 cgcttttggc gaagatccgt tacgcgtact gcgcgtggcg cgttttgctg cgcgttatgc
     9421 ccacctcggt tttcgtattg ccgatgaaac tctggcactg atgcgcgaga tgacccatgc
     9481 gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacggaaa gcgccctcac
     9541 cacccgcaat ccccaagtgt tcttccaggt actgcgcgat tgcggcgcac tgcgcgtttt
     9601 attcccggaa attgacgccc tgttcggcgt tccggcccct gccaaatggc atccggaaat
     9661 cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga gtccgcaggt
     9721 cgatgtccgt ttcgcaactt tatgccacga tctcggtaaa ggactgacgc cgccagaact
     9781 ctggccgcgt catcatggtc atggcccggc gggtgttaag ttagtggaac aactatgcca
     9841 gcgtctgcgc gtgccaaatg aaattcgcga tttagccaga ctggtggctg agtttcacga
     9901 tctcatccac accttcccaa tgctgaaccc aaaaaccatc gtcaaattgt ttgattccat
     9961 tgacgcctgg cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt
    10021 gcgcggcaga accggttttg aatcggcgga ctacccgcaa ggccgctggt tgcgcgaagc
    10081 ctgggaagtg gcacagtcag taccaacaaa agccgtcgtt gaagcgggat ttaaaggtgt
    10141 ggagattcgc gaggagctga cccgacggcg gattgcggcg gtagccagtt ggaaggaaca
    10201 acgttgccca aagcctgatt aagcacttaa atttcaatat attgcaaaca ccgtccaggt
    10261 cggataagac gcacatgcgt catatccgac actatccctc aatcaaaaga acacgacgta
    10321 caccgcagcc gccaaaataa agcgataaat ggcgaatggg ataaacgaaa tccgcttaat
    10381 caattgcagg aaggttttaa tcgctatcag cgccactacg aaggcagtga taaacccaac
    10441 ggcaaacatc gggatatcgc cggttgtcag gaagccccag cttttgtaga gatcaagcgc
    10501 cgttgcgccc atcatcatcg gcaccgccag caggaacgag aactcggaag cagcgtaacg
    10561 gctcacgccc atcagcatcc cacctgaaat ggtcgccccg gagcgagaga aacccggcca
    10621 cagcgccaga cactggaagc aaccaatcat aaacgcctga cgataggtca tatcatcgag
    10681 acccggcgca cgcggttctt ttggtttcag gcattcggcg gcaatcagca acagaccgcc
    10741 aacgaccagc gcatacatca catttatcgg gttaaacaac gacttaatcg tgtcgtggaa
    10801 caacagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg
    10861 gcctttgctt tctccttcgt gctgcaacgg gcgaccaaag tgaataccaa taaggccaaa
    10921 cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga tcacgacttc
    10981 aaaggttttc gccgtgtcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc
    11041 cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat
    11101 cagcagcgag tgcatatcgc tcatcaataa acccctaaat tataaaaatg taacgcttgt
    11161 ccgaactact gcagtatgac caggttataa ccgtttggtt taacagctgt aaaattaatt
    11221 attttctttc agattattgc cacgctcaat gatgacgcca acattcgccg cccgcgccac
    11281 tgcgcctggc ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag
    11341 ctccgccacc tcttccgcca cacgttccac cagcgcaaaa cgcgccccct cgacgtggct
    11401 gactaccgtt tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc
    11461 cgctttacgg ttatcccacg ccatttcgat atcgaacact aacttctgtt cgatggtctg
    11521 ttcccagtcg taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc
    11581 catcacgtcc tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt
    11641 atccacagat tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg
    11701 cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt
    11761 gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc cggcaatcct ggcgcaacca
    11821 atgtgttacg tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga
    11881 aaggaatgtt gcctgtctgg ggggcatatg aattaggtgt cagccccttc tggctaggct
    11941 taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcggt tttaaaggag
    12001 gaaaaggcgt tgctaccgct tttggtgcca tcgcaccaat tggctgggat cttactggag
    12061 taatggcggg aacctggtta ctgaccgtgc tattgagcgg atattcgtcg ctgggagcga
    12121 ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc
    12181 cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt
    12241 ggcgtcgtca agagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcctg
    12301 agtgattttt cgcgagtcta cataacctga tagccttcgt cgggcttgcc cagccgttgc
    12361 tggcaccatg ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc
    12421 tcccggtaaa cagcccagat attggcgctt tgtgcatact ccggcaatac tcgcaccagt
    12481 ttgccactct ccagaaacgg caacacatcc cactcggaac gcaacataat ccctttgccc
    12541 tccagcgccc attgcaacac tatctcgccg ctattggagg aaagatgccc gcttaccttc
    12601 accgattttt tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct
    12661 ttggtcacca gacaatcatg acgacttaat tcctgtaagg attgtggctg cggatatttt
    12721 tgcagatact caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata
    12781 taataatcag gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa
    12841 tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcatcagt
    12901 tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc
    12961 atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg
    13021 aggatttcca gcgcatgctc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga
    13081 gcggagcggt tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg
    13141 acaaatgcag gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg
    13201 atttctacca gtacctgcaa atctttggct aaaggccagc tattcagcat cgttaattat
    13261 ccgcagttgt gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca
    13321 atattcattc ttattttcgc gttgattcac gaactgttaa ttacatattc acgctggcaa
    13381 aaaatataaa accacatttt tagtggtagt ttgttgtggt gaatttttcc agacaaataa
    13441 agaaactgga gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac
    13501 ggagattgtc gctaacttta ccgcaatgat ttctacccga atgcctgatg acgtggtgga
    13561 taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac
    13621 gatgttcgac aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg
    13681 cgaaatcatg ttttttgtta aggtcggttc ccgtttccca ctgcttggcg agctgcaaag
    13741 catactcaaa caagccgtgg aagaggcaac cgtcaaagcg ccactgcgtc acaatgcggt
    13801 agaaattttt gacgaagtaa ataccggcaa aaataccggt agcggcgtac cgtgggtcac
    13861 ctgggacatc atccacgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg
    13921 ctgcacgcta ccaggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtagt
    13981 gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt
    14041 gggcgtgggc atcgccacct cggtggaaac cgccgccgtg ctctcgcgaa aagccatttt
    14101 gcgcccgatt ggcagccgcc atccaaatcc aaaagcggca gagctggaag tacgcctgga
    14161 agaaggactc aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat
    14221 gggcgtgcat atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtttctac
    14281 aggctgctgg gcgcatcgtc gcggcacact gctggttcat gccgatctct cctttgaaaa
    14341 tctgtctcac acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa
    14401 gatctgcaag atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc
    14461 cgtgacgttt gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac
    14521 ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa atgggagatg
    14581 gtttccgtcg gtccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgaa
    14641 cagaccggcg tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac cgaagaaggc
    14701 tgccagaaat tcaaggcgct gcatgtgatt ttcccggcag gctgcgcagt actggcggca
    14761 acccaggtgg aagagattga agaggtgcac tggacagaac tcgggatgcc ggagtcgctg
    14821 tgggtctgcc gggtcaaaga gttcggtccg ctgattgtct ctattgatac ccacggcaac
    14881 aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag
    14941 atttgcgagc acgtccacta catcaaataa ccctcccgga gaggctcacc ccctctcctt
    15001 tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg
    15061 gcggtcatcg ccattattgc tctaattccg gttcccgcag ggctggagag tcatacctgg
    15121 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    15181 gccgtggtgg cgatggtggg tatctccatt atcgccattc tctcgccgtg gctgctgttt
    15241 agcccggagc agctcgccca gccaggtttt aagttcaccg ccaaatccct ctcctgggcg
    15301 gtttctggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca
    15361 ggctatgaaa aaaccgggct ggggcgacgt atcgcgctga ttctggtgaa aaagatgggg
    15421 catcgtacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct ggcacctgta
    15481 acaccgtcca actcggcgcg cggtgcgggg attatctatc ccatcatccg taacctgcca
    15541 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    15601 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcctgacggc gatggcacct
    15661 aacttgctgt taattggcct gatgaaaagc gcatctcacg ccacactgag ttggggcgac
    15721 tggttcctcg ggatgttgcc gctcagtatt ttactggttc tgctggttcc ctggctggct
    15781 tacgtgctgt acccgccggt actgaagtct ggtgatcagg tgccgcgctg ggcagagacg
    15841 gaactgcagg caatgggccc gctctgttct cgtgaaaaac ggatgctggg gctgatggta
    15901 ggcgcactgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    15961 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    16021 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc taccggactc
    16081 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aaacggttat
    16141 tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctgctgcg ctacttcttt
    16201 gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgattgccgc tgcgctggcg
    16261 atgccggaaa tcccgcttcc cgtgttctgc ctgatggttg gcgcggcaat tggtctgggg
    16321 agcattctta cgtcatacgc caccggaccc agtccgattt actacggtag tggttatctg
    16381 ccaacggtgg attactggcg actgggggca atttttgggc tgatattcct cgtgttgctg
    16441 gtgattaccg gcttactgtg gatgcccatg gtgttgcttt aagtgaggac ggtgccggaa
    16501 gtgactctcc ggcacccgag agttttacgc agccggtaac tcagccagcg gccagcgcgg
    16561 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    16621 ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccacg
    16681 gcgttttttc atcatttcag ccagcttcgc ccgtaacgta cggttagcac tcacgccgcc
    16741 cgccatgacc agtcgcttaa agcccgtctg atccagcgcc cgcttgcact taatcatcag
    16801 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    16861 gccgttgtca cgaatggtat ttgccgcgaa ggttttcagg ccggagaagc tgaaatccag
    16921 ccccggacgg tcggtcatcg ggcgcgggaa gacaaagcgc ccggctgtac cctgagccgc
    16981 cattttcgac agtaatggcc cgccaggata atccagcccc agcagcttcg cggttttatc
    17041 aaacgcttca ccagccgcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    17101 agtcacgctg attaactgcg tatgaccgcc ggaaaccagc agcgcgacaa acggaaattc
    17161 cggtgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat
    17221 cgccggaacg tcccaggcaa acgccagaga acgccccacg gtcgcgccaa ccagcagcgc
    17281 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc
    17341 agactccttt agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc
    17401 cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt
    17461 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    17521 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    17581 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    17641 cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt
    17701 aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc
    17761 aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct
    17821 ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt
    17881 gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt
    17941 cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag
    18001 agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc
    18061 gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac
    18121 gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg
    18181 tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa
    18241 ccccatcatt caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc
    18301 acggcaacgc gatcgacttc ttgatgaact acgacaagct tgagttcgtc gaaacggtcg
    18361 aagagctggc agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc
    18421 agatcgagcg ccatcaacgg caaacgcttt atcagttgat ggacggtctg aatacgtttt
    18481 accaacaatc tttacagcaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg
    18541 gattaagcca cgaggttatc gcccgctttg cgattggttt tgctcccccc ggctgggaca
    18601 acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggca
    18661 tgttggtcac taacgatcag ggtcgcagtt acgatcgttt ccgcgagcgg gtgatgttcc
    18721 ccattcgtga taaacgcggt cgggtgattg gttttggcgg acgcgtgctg ggcaacgata
    18781 cccccaaata cctgaactcg ccggaaacgg acattttcca taaaggccgc cagctttacg
    18841 gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct
    18901 atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta
    18961 cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct
    19021 gttatgacgg cgaccgtgca ggccgcgatg ccgcctggcg agcgctggaa acggcgctgc
    19081 cttacatgac agacggtcgt cagctacgct ttatgttttt gcctgatggc gaagaccctg
    19141 acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc
    19201 cactctccgc atttctgttt aacagtctga tgccgcaagt tgatctgagt acccctgacg
    19261 ggcgcgcacg tttgagtacg ctggcactgc cactgatatc gcaagtgccg ggcgaaacgc
    19321 tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc
    19381 ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa
    19441 aacgcacgac catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgt
    19501 tggtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag
    19561 aactggtcaa cacttgtctc tctcagccag gtctgaccac cgggcaactt ttagagcact
    19621 atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag
    19681 ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc
    19741 ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag
    19801 aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa
    19861 gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat
    19921 aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc
    19981 tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct
    20041 tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg
    20101 ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca
    20161 gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc
    20221 ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc
    20281 cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat
    20341 gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc
    20401 taagcgtatt gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc
    20461 gatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga
    20521 tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca
    20581 cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga
    20641 tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgtgaaaa
    20701 atttgcggaa ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg
    20761 cagtcacgct gccgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg
    20821 cctggtgccg aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt
    20881 tcgtacgcaa gaacgtctga tcatgaagct ctgtgttgag cagtgcaaaa tgccgaagaa
    20941 aaacttcatt accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat
    21001 tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc
    21061 cctgcagaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga
    21121 tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt
    21181 tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca
    21241 gttccttgac ctgattcagg aaggtaacat cggcctgatg aaagcggttg ataaattcga
    21301 ataccgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac
    21361 ccgttctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat
    21421 caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc
    21481 ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat
    21541 cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg
    21601 ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct
    21661 gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg cgtgaagcga aagttctgcg
    21721 tatgcgcttc ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt
    21781 cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca
    21841 cccgagccgt tctgaagtgc tgcgtagctt cctggacgat taatcggtag gccggatcag
    21901 gtgttacgcc gtacccggca ctagtccctc cgcacaaacg ccaccttttc ggtggcgttt
    21961 tttatcgccc acgcactacc agcgcctggt ccagctcgcg atacgcttca accagtttct
    22021 ccagtgaaac gcgacttaaa ccactgggat ttggcagcac ccaaatctgc gtcgaaccaa
    22081 tggtgagcgt ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt
    22141 gtttgcccag aatcgccaac gcctgcggct gataatcttc aattttttca atcagcttac
    22201 gcccgcctgc gtgtagctcc tgctttgaaa cttcattggc ttgcaccgtt ggacggtcta
    22261 ccagtttggt gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact
    22321 gacggtcggt aaacccggcc tgatatatca ccttccagaa gcgatttgcc ggatgagcaa
    22381 agggaaaacc agtcccggcg gatgaaagcc cagggttgat accgcaaaac acgacccgta
    22441 accctggagc caaaatatcc tcaaccatat ttactccttt ctaacatcac caggaaaagt
    22501 ataacggatt gaaaatacat tgtttataaa aacagcaggc gcgcggtaat ggctggattg
    22561 cgacacggag ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg
    22621 cgactcataa tcgcttggtc gctggttcaa gtccagcagg ggccaccaga catagcaaag
    22681 gctgacgaga aatcgtcagc ctttttcttt ttatatatca gttactttgc gtgccagtaa
    22741 gccgctgcac gtacccgctg tggatcatac tgttccgctt caaagcggcg gcttaaattc
    22801 ttaacgactt tgccttcgcc ggttatccag atgaagtaat catcggcagg gatttgcatc
    22861 tgcgccagac gcgcatctac tgcctgctca tcatgtgcca gccattcgat attaaaacca
    22921 tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca cgctaaccag cgcgctaact
    22981 tgcggtttaa cggcaagttt gctcaacgtt tccaggcggc agcgcagtgc gggcattccg
    23041 gattcatcgc agacatacag ctgatacgcg taatcctctg gcaccaccag cgaaccgcgc
    23101 ggacctgcca ccgtgagttt atcgcccggt tgcgcctgca tcgcccagcc gctggcgacg
    23161 ccaccttcgt gaataaagaa atcaatcgcc agttcatggc gtagttcgtc atacagcggt
    23221 gtatagtcac gcgacggtgg gcgtggtcct tccggccaga cgatgccctc ttccgttact
    23281 gttggcggca caaagtgagc atcagattga ggaaagaaga gtttgctatg atcgtcaaag
    23341 ccgcgcgacg taaagccgtc cagcgcctcg ccgccgagga caatgcgttg aaaaccggcg
    23401 ctgatgcgct caacgtgcaa tacagtcagt tcacggaagc gcagatcatt gcgaacgcgc
    23461 tgcgggtagc ggggggagtt attcattgtt atcgccttca tgatggtaat cagatatatc
    23521 taaat
//
LOCUS       DARNVM010000067        22534 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.067, whole genome shotgun sequence.
ACCESSION   DARNVM010000067 DARNVM010000000
VERSION     DARNVM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.067"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            70..612
                     /locus_tag="SI621_002376"
     CDS             70..612
                     /locus_tag="SI621_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4065087.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            693..1367
                     /locus_tag="SI621_002377"
     CDS             693..1367
                     /locus_tag="SI621_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEI4065088.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1383..3863
                     /locus_tag="SI621_002378"
     CDS             1383..3863
                     /locus_tag="SI621_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEI4065089.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCISREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSEGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAINKQQGLSCTITFGKEIDESRNYICQ"
     gene            3879..4913
                     /locus_tag="SI621_002379"
     CDS             3879..4913
                     /locus_tag="SI621_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HEI4065090.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSSNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLITLESTPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLWGSSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4995..5333)
                     /gene="rcnB"
                     /locus_tag="SI621_002380"
     CDS             complement(4995..5333)
                     /gene="rcnB"
                     /locus_tag="SI621_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HEI4065091.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5552..6376)
                     /gene="rcnA"
                     /locus_tag="SI621_002381"
     CDS             complement(5552..6376)
                     /gene="rcnA"
                     /locus_tag="SI621_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HEI4065092.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWNGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6497..6769
                     /gene="rcnR"
                     /locus_tag="SI621_002382"
     CDS             6497..6769
                     /gene="rcnR"
                     /locus_tag="SI621_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HEI4065093.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6861..6957
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6992..7780
                     /gene="thiM"
                     /locus_tag="SI621_002383"
     CDS             6992..7780
                     /gene="thiM"
                     /locus_tag="SI621_002383"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEI4065094.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAIEQAKRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTPEVQA"
     gene            7777..8577
                     /gene="thiD"
                     /locus_tag="SI621_002384"
     CDS             7777..8577
                     /gene="thiD"
                     /locus_tag="SI621_002384"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEI4065095.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTQNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8642..9460
                     /locus_tag="SI621_002385"
     CDS             8642..9460
                     /locus_tag="SI621_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEI4065096.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIHPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            9512..10258
                     /locus_tag="SI621_002386"
     CDS             9512..10258
                     /locus_tag="SI621_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4065097.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSNVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10232..11197)
                     /locus_tag="SI621_002387"
     CDS             complement(10232..11197)
                     /locus_tag="SI621_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEI4065098.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11194..12198)
                     /locus_tag="SI621_002388"
     CDS             complement(11194..12198)
                     /locus_tag="SI621_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEI4065099.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGCIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12195..13472)
                     /locus_tag="SI621_002389"
     CDS             complement(12195..13472)
                     /locus_tag="SI621_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065100.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13728..14780
                     /gene="fbaB"
                     /locus_tag="SI621_002390"
     CDS             13728..14780
                     /gene="fbaB"
                     /locus_tag="SI621_002390"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEI4065101.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15008..15862
                     /gene="gatY"
                     /locus_tag="SI621_002391"
     CDS             15008..15862
                     /gene="gatY"
                     /locus_tag="SI621_002391"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HEI4065102.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEAYAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKYI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15891..17153
                     /gene="gatZ"
                     /locus_tag="SI621_002392"
     CDS             15891..17153
                     /gene="gatZ"
                     /locus_tag="SI621_002392"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HEI4065103.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADRVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITRVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQEIIAPENRSGCLAVIEEVMLDEPQYWKKYYRPGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVEKMMVNLEGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            17163..17615
                     /gene="gatA"
                     /locus_tag="SI621_002393"
     CDS             17163..17615
                     /gene="gatA"
                     /locus_tag="SI621_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HEI4065104.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17646..17930
                     /gene="gatB"
                     /locus_tag="SI621_002394"
     CDS             17646..17930
                     /gene="gatB"
                     /locus_tag="SI621_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HEI4065105.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            17934..19289
                     /locus_tag="SI621_002395"
     CDS             17934..19289
                     /locus_tag="SI621_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEI4065106.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFAHDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            19337..20377
                     /gene="gatD"
                     /locus_tag="SI621_002396"
     CDS             19337..20377
                     /gene="gatD"
                     /locus_tag="SI621_002396"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEI4065107.1"
                     /translation="MKSVVNDTDGIVRIAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMSIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMLTFNSLEMSAP
                     QMQGVLRELRFNQLILETAGVPKTVELAVEIAGPHAQLALVGTLHQDLHLTSTTFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            20483..21256
                     /locus_tag="SI621_002397"
     CDS             20483..21256
                     /locus_tag="SI621_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4065108.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21338..22237)
                     /gene="yegS"
                     /locus_tag="SI621_002398"
     CDS             complement(21338..22237)
                     /gene="yegS"
                     /locus_tag="SI621_002398"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HEI4065109.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VRIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         5923 a         5178 c         5735 g         5698 t
ORIGIN      
        1 tgagaaattt gtttccaaga gatacagact cttaacaacc aaaaaataaa ttttattaag
       61 gaacatatta tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc
      121 gctggggtat ttgccgcaga cgttgatacc ggaacattaa ccattaaagg gaatattgca
      181 gaatctccgt gtaaattcga agcgggtggc gattcagtaa gtattaatat gccaactgta
      241 ccaaccactg tctttgaagg taaagctaaa tactcgacct atgatgatgc agtcggtgta
      301 accagcagca tgttaaaaat tagctgtccg aaagaagttg ctggtgtaaa actctcgttg
      361 attaccaacg ataaaataac cggaaacgat aaggcgatag ccagtagcaa cgataccgtg
      421 gggtactatc tctatttagg tgataacagc gatgtcctgg atgtttctgc accttttaac
      481 attgagagtt ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg
      541 aagctgacag ataattcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg
      601 gcgcaggatt aataaataaa acttcaagtt tctccaggta ataaacatgg ccgctatccc
      661 catggcggcc ttttaattta agaggcatta agatgaaagg attattagct ttactcattt
      721 cttctatggt ccttcctgca catgccggta ttgttatcta cgggacgcgt attatttacc
      781 cggcagaaaa taaagaagtg atggtgcagt tgatgaatca gggaaaccgt tcttcgctgc
      841 tgcaggcgtg gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt
      901 tcatgttaac gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca
      961 aaattatgcc gaataaactg cccactaata aagagagcat tttttatctg aatgttctgg
     1021 atattccacc aaatagtcca gagcaagagg gcaagaatgc actgaaattt gcaatgcaaa
     1081 acagaattaa attgttttac cggcctgcgg gtattgcgcc agtaaataaa gcgacattta
     1141 aaaagttact ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagcaaatt
     1201 gggtaacgat ttcggatgtc aaagctaata atgtcaaagt caattatgaa actattatga
     1261 ttgccccctt agaaagtcag agtattaatg tcaaaagtaa taatgcgaat aactggtatc
     1321 tgaccattat cgatgaccat ggcaactata ttagtgacaa aatttaattg caggagctgc
     1381 ctatgttgag aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag
     1441 cttatgcagc tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc
     1501 aggttgcaaa tattcgtctt gatgataatc aacccttgcc ggggcagtat gacatcgata
     1561 tttatgtcaa taagcaatgg cgcgggaaat atgagatcat tgttaaagac aacccgcaag
     1621 aaacatgtat atcaagggaa attatcaagc ggttaggcat taataccgat aactttgcca
     1681 gcggtaagca atgtttaaca tttgagcaac ttgttcaggg tgggagctat acctgggata
     1741 tcggagtttt tcgtctcgat ttcagtgtcc cgcaggcgtg ggtggaagaa ctggaaagtg
     1801 gctatgttcc accggaaaac tgggagcggg gtattaatgc gttttatacc tcttattatg
     1861 tgagtcagta ttacagcgac tataaagcgt cgggtaatag caagagtaca tatgtacgtt
     1921 ttaacagcgg gttaaactta ctggggtggc aactgcattc tgatgccagt ttcagtaaaa
     1981 caaatagcaa tccagggggg tggaaaagca ataccctgta tctggaacgt ggatttgccc
     2041 aacttctcgg cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg
     2101 ttcgcttcag cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaga
     2161 attttactcc acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga
     2221 acggttttgt ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc
     2281 agttggccgg tggtggagca gatcttgatg tcagcgtgaa agaggcggac ggttcggtaa
     2341 ccacctatct ggtgccttat gcagcggtgc cgaatatgct gcaacctggc gtgtcgaaat
     2401 atgattttgc ggcgggtcgt agccatattg aaggggcgag caaacaaagt gattttgtcc
     2461 aggcgggtta tcagtatggt tttaataact tattgacgct gtatggcgga tcgatggtcg
     2521 cgaataatta ttacgcgttc actttgggga ctggctggaa tacacgcatt ggtgccattt
     2581 ccgtcgatgc cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa
     2641 gttatcaaat tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg
     2701 cctggcgtta ttcgtcgcgt gattaccgga catttaacga tcatgtttgg gcaaacaata
     2761 aagataatta tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg
     2821 attttggccg taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg
     2881 gttctgtgtc cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg
     2941 attatcagtt gagttattcc aacaacctgc gacggataag ctataccctc gcggcaagcc
     3001 aggcttatga cgagaatcat catgaagaga aacgttttaa tatttttata tcgattccct
     3061 ttgattgggg tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga
     3121 cgtttgatga tcagggtttt gcctcaaata atacgggatt atcgggaacc gttggaagcc
     3181 gggatcaatt taactatggg gtcaacctga gtcatcagca tcagggaaat gaaacgacag
     3241 ctggagcgaa tttaacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt
     3301 cgagtactta tcgacaggct ggagccagtg tttcaggggg cattgtcgcc tggtcggagg
     3361 gcgttaatct ggcgaaccgt ctttccgaaa cgtttgcagt gatgaatgcg ccaggaatta
     3421 aagatgctta tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat
     3481 acgacggaat gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata
     3541 gcgaagcaga attgcgcggt aaccggaaaa ttgccgcacc ttatcgcggc gcggttgtac
     3601 tggttaattt tgataccgat cagcgcaagc cctggtttat aaaagcgtta agaacggatg
     3661 ggcaaccatt aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg
     3721 tcggccaggg aagccagtta tttattcgca ccaatgaagt accgccgtcg gttaatgtgg
     3781 caattaataa gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa
     3841 gtagaaatta tatttgccag taatagtcag gtggttctat gaaaattcgc ataatgctat
     3901 ttatattaat gatgatggtt atgccagtga gctatgcagc atgttatagt gagttatctg
     3961 ttcagcacaa tttggttgtt cagggggatt ttgcacttac tcaaacacaa atggcgacct
     4021 atgagcataa ttttaatgat tcgtcatgcg tgagttcaaa tactatcacc cctatgagcc
     4081 cgtcggatat tattgttgga ctttataacg ataccattaa attaaattta cattttgaat
     4141 ggaccaataa aaataacatc acgttgtcaa ataatcagac cagtttcacc aatggttatt
     4201 cggtaacggt gacacctgca gccagtaatg caaaagtgaa tgtttctgcg gggggcggcg
     4261 gttcagtgat gattaatgga gttgcgacat tatccagtgc ttcatcatcg acacgcggga
     4321 gtgccgcagt acaatttcta ctgtgtttat taggtggcaa gtcatgggat gcatgtgtaa
     4381 atagctacag aaatgcattg gcacaaaatg caggtgtcta ttcctttaat ctggcattgt
     4441 catataaccc gataacaacg acctgcaaac cggacgattt attaattact ttagagagta
     4501 ctcccgtttc acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaggag
     4561 atattattct gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cgaaaaatgc
     4621 aggtgtattt atcaagttct gatttgtggg gcagtagtaa cacgatcctg aaaggtgcgg
     4681 aagataatgg cgtagggttt attcttgaaa gtaatggttc gccagtcaca cttttaaata
     4741 tcactaacag cagtaaagga tatacaaatt taaaggaagt tgctgcgaag tcaaaactta
     4801 cagatacaac ggtttcaatt ccgataactg ccagttacta cgtctacgat acaaacaaag
     4861 ttaaatctgg cgcactggag gcaaccgcat taatcaacgt gaaatacgac taataacaag
     4921 caagacgagc aagtggctaa taataaaaaa taacaaggtg tctgcatttc cccctcatga
     4981 tgagggggct ttttttagcg atgataaaaa atctcaccgt cgtaggcttt aatgatttta
     5041 ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat aggtccagtg cgtcccggca
     5101 tcaggcgcgg gcaggttacg taactgccac tgcttaatgt tgtactcatc ggtgcggtac
     5161 atctctggga cggtatcgcc tggtttgaaa tgcttaaagt cagcaatgaa actgcttaac
     5221 tcatacttag aaattcccga ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg
     5281 gcactggtaa ccagcaaaag cgcacccagc aacatcttat ttttaatggt cataaactct
     5341 ccactttggg gatctgtaat atcagtcgca tatctttcct gccatacaat ataactggca
     5401 agcgatattt tgttagtgat cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta
     5461 aaagcatcct tagcgagcag agagtcagcg gaaatagctg atttaagaat catcctgggc
     5521 tgaagataac atgcaagcct aaaggattga gttatcgcat tatgcccatg aagccatgta
     5581 cgcccatata cacaccaact aagccaatca acagactgga aaaatagggg gcgcgtttag
     5641 cgagagtgtt aaatccgttc cagcgttttg cgacctgctg aacgctgatt gctgcgccaa
     5701 cgcctacggt gacaagcgtt aacgccaggc caatgctgaa actgacgacc agtgttgcgc
     5761 ccagtgtcag ggctttcaac tgaatgcata tcaacagcac ggtaattgct gccgggcagg
     5821 ggataaggcc accggttaag ccaaatagca aaatttgcca gttggtgacc tctctaccat
     5881 caaagcgtcg tttaatgtca ttggcatggg ctcgtgcatg ggcatcctga tactcgccat
     5941 gttcgtgatg gtgatgatgt ccatggtcgt ggtgatcttc gtgatcgtgg tgatgatgct
     6001 catgatcatg ctcgtgcata ttctccagcc agtttcgttc gccgcgccag gtacgccaga
     6061 acatccagaa cgcggtgcca ataatgatca ctgcggaaat caactggagc cacggttctg
     6121 ctgattgagc agtaaagcgc ttgctgatca ccatcccgcc aaaggcaatc aaccagacca
     6181 ctgcggtatg cgaaatggtt gctgccagtc cgagcatcac cgcctgttta atggtgcctt
     6241 tgatggcgat gataaacgcc gccatcatcg tttttgagtg ccctggttcc aggccatgaa
     6301 gcgcaccaag taagatggcg ctggggatga agaaccaggc gtttccttgc tgaagaagag
     6361 ttgtaaattc ggtcatgata ataattctta gtattaattc ggcaatctga ttctactccc
     6421 ccccagtacc tgatactacc ccccagtaga ttaatagtgc tatgattttt catattctta
     6481 ttaaccaggt gttgccatgt ctcataccat ccgtgataaa cagaaactga aagcgcgtgc
     6541 cagtaagatt cagggccagg tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg
     6601 cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga
     6661 agtgattaaa ggtcatctga cggaacacat cgttcaccag ggggatgagc taaaacgtga
     6721 agaagatctg gatgtcgttc tgaaggtgct ggattcttat atcaaataat ttattaacgc
     6781 gattgtaaaa ctgcggtttt tactcgttta caacgcgtgc gctggacatt accatcctcc
     6841 tctgcgattt atcatcgcaa tcaaacgact cggggtgccc ttctgcgtga aggctgagaa
     6901 atacccgtat cacctgatct ggataatgcc agcgtaggga agtcacggac caccaggtca
     6961 ttgcttcttc acgttatggc aggagcaaac tatgcaagtc gacctgctga gttccgcgca
     7021 atctgcgcac gcgttacatc tttttcacca acattcccct cttgtgcact gcatgaccaa
     7081 cgatgtggtg caaaccttta ctgccaatac cttgctggcg ctcggtgcat ccccagcgat
     7141 ggttatcgaa accgaagagg ccagtcagtt tgcggctatc gccagtgcgt tgctgattaa
     7201 cgttggcacg ctgacgcagc cacgcgcaca ggcgatgcgt gccgccattg aacaggcaaa
     7261 acgctctcaa acaccctgga cgcttgatcc agtagcggtg ggtgcactcg attatcgccg
     7321 ccgtttttgt ctggaacttc tgtctcataa gccaaccgcc atacgtggta atgcttcgga
     7381 aatcatggca ttagctggcg ttgctaatgg tggacgggga gtggatacca ctgacgccgc
     7441 agctaatgcg atacccgctg cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt
     7501 cactggcgag atggattatg ttaccgatgg acatcgtatc attggtattc acggtggcga
     7561 tccgttaatg accaaagtgg taggaactgg ctgtgcatta tcggcggttg tcgctgcctg
     7621 ttgtgcgcta ccaggcgata cgctggaaaa tatcgcatct gcctgtcact ggatgaaaca
     7681 agccggagaa cgcgcagtcg ccagtagcga ggggccaggc agttttgttc cacatttcct
     7741 tgatgcgctc tggcaattga cgccggaggt gcaggcatga aacgaattaa cgctctgacg
     7801 attgccggta ctgatccgag tggtggtgcg gggattcagg ccgatcttaa aaccttctcg
     7861 gcacttggcg cttatggttg ctcagtgatt actgcactgg tggcgcaaaa tacccgtggc
     7921 gtacagtcgg tgtatcgcat tgagcctgat tttgtcgctg cccagctcga ttcggtgttc
     7981 agcgatgtgc gaatcgacac cactaaaatc ggtatgttgg cggaaaccga tattgttgaa
     8041 gcggtggcag aacggttgca acgttatcag atccaaaacg tggtactcga caccgttatg
     8101 ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg ttgctacgct gcgcagtcga
     8161 ttattgccac aggtttcatt aataacgcca aacttgcccg aagctgctgc cttgctcgac
     8221 gcgccacacg cgcgcaccga acaggaaatg ctggaacaag ggcgatcgct gttggcgatg
     8281 ggctgtggcg cagtgctaat gaaaggtggt catctggatg atgagcaaag ccctgactgg
     8341 ctgtttactc gtgaggggga acaacgattt actgcaccgc gcattatgac ccaaaacacc
     8401 cacggcactg gttgtacgct ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac
     8461 tgggctgaca ccgtacagga ggcaaaaagc tggctttcat cggcgttagc ccaggccgac
     8521 acgctggaag ttggtcacgg tattggtccg gttcaccact tccacgcctg gtggtgagca
     8581 ccgtactata gctgctttgg ttattgtcag cctttggcac tgacattcag ggatgaaacc
     8641 aatgcagcta agaatcacca gtcgaaaaaa actcaccgct ttattatgcg ctcttgggct
     8701 aatttccatc gttgcgattc atccgcgtca gacggtgaat tttttctatt cgacagcaat
     8761 tcagattaaa gactacatcc acttctacgg ttatcgcccg gttaaatctt tcgctattcg
     8821 cattcctgcc agttacacca ttcacggaat agatgtttcg cgctggcagg agcgaatcga
     8881 ctggcagcgg gtggcaaaaa tgcgcgataa cggtattcgc ttgcaatttg cctttatcaa
     8941 ggcgacggaa ggcgaaaagc tggtggaccc ctatttttcg cgtaactggc aactaagccg
     9001 cgaaaatggc ctgctgcgcg gggcgtatca ttatttttcc ccgtcggtat ccgcttcagt
     9061 tcaggcgaga ttatttctgc aaacggtgga tttctcacaa ggcgatttcc ctgccgtgct
     9121 ggacgtagaa gaacggggaa aattatcggc aaaagaatta cgcaagcggg taagtcagtg
     9181 gctaaaaatg gtcgaaaaaa gtacggggaa aaagccgatt atttactcag gagccgtttt
     9241 ttatcacacg aatctggcgg gctatttcaa tgaatatccg tggtgggtgg ctcattacta
     9301 tcaacgtcgt ccggacaatg acggaatggc ctggcgcttc tggcagcatt ccgaccgtgg
     9361 acaggtagat ggcatcaatg gtccggtgga ttttaatgta tttaatggca cggtggagga
     9421 gttgcaggca ttcgttgatg ggattaaaga aacgccttaa ataagaagca gtgatatact
     9481 cccaccagac tccctggcag gaaaaagaca aatggaacaa gcgcataccc agctgattgc
     9541 ccaactgaac gaacgtattt tagcggcgga taacacgccg ctttatatta agtttgccga
     9601 aacggtaaaa aatgccgtgc gcagcggggt gctggagcat ggcaatattt tgcccggtga
     9661 gcgtgattta agtcagttaa ccggcgtttc gcgcattacg gtgcgcaagg cgatgcaggc
     9721 gctggaagaa gagggcgtgg tgacgcgttc gcgtggttac ggtacgcaga tcaacaacat
     9781 cttcgaatac tcgttgaaag aagcgcgagg tttttctcag caggtggtat tgcgcgggaa
     9841 aaagcccgat acactatggg ttaacaagcg tgtcgtgaaa tgtcccgaag aagtcgcgca
     9901 gcagctagcg gtcgaagcag gaagtaatgt ctttttgctt aagcgtattc gctatgtcga
     9961 tgaagaagcg gtatcaattg aggaatcgtg ggttcctgcg catttaattc atgatgttga
    10021 tgccatcgga atttctcttt atgactactt ccgcagccag cacatttacc cacaacgtac
    10081 gcgttcccgc gttagcgccc ggatgccgga tgccgagttt cagtcacata ttcagttaga
    10141 tagcaaaata ccggtgctgg tgatcaagca agtggcgctt gaccaacagc aacggcctat
    10201 tgagtacagc atcagccact gtcgcagcga tctatacgtt tttgtgtgcg aggagtagtt
    10261 cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac aacccacgac gccactgcat
    10321 tgcccagtaa tacggcatcc gccagtggca gaccagaggc tagcccggca agtacgccac
    10381 ctgcatgact gtcgcccgcc ccaatggtgt ctactacttg cgttggaaat gccggaatgc
    10441 agccagaagc gtcgttgctg aaataccatg cgccctcttt atcgagacga atgatcaacg
    10501 gcgcggcaaa tttctcctgc cattgcttgc caagtgttgt tatctctgcg gataaagcaa
    10561 aacgttcggc ggcaatctca gcctcctgac gattgagtga cactaaaggt cgacaggcca
    10621 tgatccgcgc cagcaatgca tccgggatat cgccaatacg tgggccaaaa tcgataaacg
    10681 gcgtcacgtc ttgcagcttt tccagccatt ccactaataa ttcgccgcag ggcgaggcca
    10741 gttgataacc ggaaaaatag agcaggctgc caggcgcaac ggttaatcgc gccagccact
    10801 ggcgattcca ctgattttcc acaccgctga atgacataaa agtgcgttcg ccatccggct
    10861 caaccagcgc cagacaccag ccgttatcac cttcggcgtt atcgatcagg ctgattaagc
    10921 cctcttttgc catccggttg cgaataatct ccgcccacac gccctgaccg agcggcaagg
    10981 cgttacccgc ttcgatgccg aggcgcttta acgccacggc aatattcagt gcgcagccgc
    11041 caacgttaac gctctgctgt ttcagttcga tatcgcaccc acgccagggg agggcataag
    11101 cgtcggcgat cacatcaatg accgccgcgc caatcaccat aactggctga cgcgtggtta
    11161 attcaggcag cagcgtgtgt aatcgagcgc cgctcatacc gcctcccgtt gttgacgata
    11221 tttcgccagc gctgtggcat agcggttgaa atcaagctga tttaccgcat ccagctccgc
    11281 ctttaacgca ggatcgatag cgttaatgcc atgcaacgcg ccgcagattg ccgtcgccat
    11341 agcaccgatg gtgtctgtat cgccgccaag gttagcgcag aggacggcgc aacggttcgg
    11401 atcggtttgt gccagttcaa ccagcgcaat ggcgcacgga acggactcaa tagtgctggt
    11461 acctgcgcca acgacctggt aaagctgttc gctggcggat tcggtgccgt cggcattgcg
    11521 cacaattttc agcgcaatct ccagccgcgc cgccagtgag gcgctgaagg tggtgatgcg
    11581 tttttgctgt gcatgtcgcg caatcgaggg tagagaatca acaatcgctg accagctttc
    11641 tccgtcaatg gcacgggaaa tcgcccatgc gatgactacc gcacctgcaa ccgccagatc
    11701 ggatttatgg gtcgggctgg acgccagcgc cacatcatca ataaaggaat caacatcgcg
    11761 cgccggaagc aaacaaccta atggcgagac gcgcatcgct gcgccgttgg tcacgccatt
    11821 gttttccagt tcagcaacgg gtttaccgtc gcgaatggcg ttaagcgcaa tcttcgaggt
    11881 cggacctagt acgtttttgt taaaggcgtc gaaacgcagc gcccagtcga gaatattacg
    11941 cccaatcaga tccggatcga tcttgccttt acgttccagt aacgcatccg ccagacacag
    12001 cgccatcgag gtatcgtcgg tgaattcggc gcggttaaaa taacaggccg cgttattctc
    12061 ctttggtcca ggcagaaaac ggtcaataca gccaaagtgt gctttaacgc ggctgcgtgg
    12121 ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc tgcccataaa gagcaccgag
    12181 aatacgttct gttttcattt aacttcccct tgtgtcaacg caatatcgcg atcatcgacc
    12241 ttgatagccg taatttcgtt gtcggattcg cggaaaaaaa tcatgaacag cacggcgata
    12301 atggcaatca tcaccgcgcc aaaagtccac atcccggccc agttgaaagt cagtccgttt
    12361 accggttcct gataagcgaa catcttttcc atcatcacac cgccaagacg atagccgagc
    12421 aaactgccga agccctggca gcagagcgtg atcagcccct gcgcggcggt acgcatatgc
    12481 acgggggctt ttttatcgac atagatgtaa gcggtaacgt agtaaaaatc gtaacttacg
    12541 ccgtgaagca aaatgccgag gaacagtaac gcgtaggtga aatattcatc cgcactaccg
    12601 taaataaaga agccatagcg gatcgcagcg gtgaccagac caagtaataa tacctttttg
    12661 ataccaaagc gtttggtaaa aaacggcaat gccagcataa agaagatttc agagaactgg
    12721 ccgagcgtca tccagccagt ggcgtttttc atgccaactt cggtcagata accgttggca
    12781 aagatgtaat agaacgccag tggcatcgca aacagaaatg aacagaagaa aaagacgagg
    12841 aagtttttat cgcgcagcag gatcagcgca tccaggccga gcatgacttt aatgtccatt
    12901 ttgccggtgc tttttggcgg cgtgtcgggc aggaaaaacg caaacacacc gagcagagca
    12961 gaacttccgg cggtaatcag cagcgggatg ttagtcggag agatatcggc ataacccagc
    13021 atttgcggca aaaatccaca tgccagaccg gaggcaatcc agccgatagt gcccatcaca
    13081 cgaatgcgtg ggaaatcacg ctccacatcc ggcacgttgg caaaagcgat actgttagtc
    13141 agcgcaatgg tcggcatata ggttagcgag taggccagca gtaacgggaa gaacccggca
    13201 aaagtggtct gttgcgcagc gaaatacatc agcgccgcac cagcgaacat caataccgcc
    13261 agcactttct gcgccgagaa aaagcggtca gtgatggagc caaccagaat tggcgacagg
    13321 atcgccgcaa tggcggtaca ggcatacgac cagccaattt ctccggcact aaaaccgctt
    13381 ttacttaacc acaaccacaa tggcacaaac cacgcgcccc agataaacca ttcaacaaac
    13441 atcatgaacg acagctttgc tgttgttttc atcttttaat tccttcatga caggtaatgt
    13501 aaggtacgcg atgacaatac catttaataa taccttttaa atacctttgc ggtgaaaact
    13561 tgaccgcgat aacatttttt ctgatgaatc gagccaacag aaaacgctga aaaacatcca
    13621 aaagatggaa aaactcgcct gtaacgacca ggattatgat tgcccgcaaa aggcgggcca
    13681 ggacaatcca tagccgatat ccaatcggaa tttacgggag catagtaatg acagatattg
    13741 cgcagttgct tggcaaagac gccgacaacc ttttacagca ccgttgtatg actattcctt
    13801 ctgaccagct ttatctcccc gggcatgact acgtagaccg cgtgatgatt gacaataatc
    13861 gcccgccagc ggtgttacgt aatatgcaga cgttgtacaa cactgggcgt ctggctggca
    13921 caggatatct ttctattctg ccggttgacc agggcgttga gcactctgcc ggagcttcat
    13981 ttgctgctaa cccgctctac tttgacccga aaaacattgt tgaactggcg atcgaagcgg
    14041 gctgtaactg tgtggcgtca acttacggcg tgctggcatc ggtatcgcgg cgttacgcgc
    14101 atcgcattcc attcctcgtc aaacttaatc acaacgagac gctaagttac ccgaatacct
    14161 acgatcaaac gctgtatgca agcgtggagc aggccttcaa catgggcgcg gtggcggttg
    14221 gtgcgactat ctattttggt tcggaagagt cacgtcgcca gattgaagaa atttctgcgg
    14281 cttttgaacg tgcgcacgag ctgggcatgg tgacagtgct gtgggcctat ttgcgtaact
    14341 ccgcctttaa gaaagatggc gttgattacc atgtttccgc cgacctgacc ggtcaggcaa
    14401 accatctggc ggcgaccata ggtgcagata tcgttaaaca aaaaatggcg gaaaataacg
    14461 gcggctataa agcgattaat tacggttata ccgacgatcg cgtgtacagc aagttgacca
    14521 gcgaaaaccc gattgatctg gtgcgttatc agttagctaa ctgctatatg ggccgcgcag
    14581 ggttgataaa ctccggcggt gctgcaggcg gtgaaactga cctcagcgat gcagtgcgta
    14641 ctgcggttat caacaaacgc gctggcggaa tggggctgat tcttggacgt aaggcgttca
    14701 agaaatcgat ggctgacggc gtgaaactga ttaacgccgt gcaggacgtt tatctcgata
    14761 gcaaaattac tatcgcctga tacgccttat acgaacagca ctaccccgta tgacggaaaa
    14821 ggcgtttacg tcgcatccgg cataaaaaca cgcgcacttt gctacggctt ccctatcggg
    14881 aggccgtttt tttgcctgtc attctgatta attttcgaat tgtcgttttt gtgatcgtta
    14941 tctcgacatt taaaaacaaa taatttcatt atattttgaa atcgaaaaca aacgacagga
    15001 tatgaaaatg tacgtggtat cgacaaagca gatgctgaac aacgcacagc gcggcggtta
    15061 tgcggttccg gcattcaata ttcacaatct cgaaacgatg caagtggtgg tagaaaccgc
    15121 tgccaacctg catgcgccgg tcatcatcgc cggaacgcct ggcacattta ctcatgccgg
    15181 tacagaaaat ttgttggcgc tggtcagcgc gatggcgaag caatatcacc atccactggc
    15241 gattcatctc gaccatcaca cgaaatttga cgatatcgct cagaaggttc gttctggcgt
    15301 gcgctcagtc atgattgacg cctcgcattt gccttttgcg caaaatattt cacgggtcaa
    15361 agaggtggtg gatttttgcc atcgctttga tgtcagcgtc gaagcggagc tggggcaact
    15421 tggcggccag gaagatgatg tgcaagtcaa tgaagcctat gctttttata ccaaccccgc
    15481 tcaggcgcgt gaatttgccg aggcaaccgg aattgattcc ctggcggtcg ccatcggcac
    15541 ggctcatggt atgtatgcca gcgcaccggt gcttgatttt tctcgactgg agaacattcg
    15601 ccagtgggtg aacttaccgc tggtgctgca tggcgcgtca gggttatcga ctaagtatat
    15661 tcagcaaacc atcaaactgg ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc
    15721 cttctcgcag gcgttaaaaa attacctgac cgagcacccc gaagcgaccg atccccggga
    15781 ttatttgcag tcggctaaat ccgcaatgcg cgatgtggtg agcaaagtga ttgccgattg
    15841 tggctgcgag ggcagggcat aacgcacctg ccatttaaca aggaaaaaac atgaaaacgt
    15901 taattgcccg gcataaagct ggtgaacata ttggcatatg ttcagtctgt tctgcccatc
    15961 cgttggttat cgaagcggcg ctggcatttg atcgcaacag cacgcgcaaa gtgctgattg
    16021 aagcaacgtc aaaccaggtc aatcaatttg gcggttatac cggcatgaca ccggcggact
    16081 ttcgcgaatt tgtttttgcg attgccgata gagttgggtt tgcgcgcgaa cgcattattc
    16141 tcggcggcga ccatctgggg ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg
    16201 aaaaatccgt cgagttggta aaggcatatg ttcgtgccgg cttcagtaaa attcatcttg
    16261 atgcgtcaat gtcctgcgcg ggggatccca taccgttagc accagaaacg gttgcggaac
    16321 gagctgctgt gctttgcttt gctgcggaaa gtgtggcgac agattgccag cgtgagcaac
    16381 tgagctatgt cattggcacc gaagttccgg ttccgggcgg tgaggccagc gccattcagt
    16441 cagtacacat tacccgggtt gaagatgccg ccaatacttt acgtacgcat caaaaggcct
    16501 ttattgcccg tgggctggca gaggcgttaa cacgcgtgat tgccattgtg gtgcagccag
    16561 gcgtggaatt tgatcacagc aatattatcc attatcagcc gcaggaagca cagccgctgg
    16621 cgcaatggat agaaaacacc cgaatggttt atgaagcaca ttctaccgat taccagaccc
    16681 ggacggctta ttgggaatta gtccgcgatc actttgcaat attgaaagtc ggtcccgcat
    16741 taacctttgc tttacgtgag gcgatatttg cgttggcaca aattgagcag gaaattatcg
    16801 cccccgaaaa tcgcagcggt tgcctggctg taattgaaga agtgatgctc gacgaaccgc
    16861 agtactggaa gaaatattat cgcccgggtt ttaacgattc attactggat attcgttaca
    16921 gcctgtcgga tcgtattcgt tattactggc cgcatagtcg gattaaaaat agcgtcgaaa
    16981 agatgatggt gaatctggaa ggcatggaaa tccctctggg catgattagt cagtatcttc
    17041 ctaaacaatt tgaacgcatt cagtccgggg aattatcagc aataccgcat cagttgatta
    17101 tggataaaat ttatgatgtt ttgcgcgcct atcgctacgg ctgtgcggaa taaggacggt
    17161 atatgactaa cctgtttgtt cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa
    17221 cccatatcgg taatgagatg ctcgcgaaag gtgtggttca tgatacctgg ccacaggcat
    17281 taattgccag agaagcagaa ttccctaccg ggataatgct tgagcagcac gccattgcaa
    17341 taccgcattg tgaggcgatt catgctaagt cgtcagccat ttatctgtta aggccaacaa
    17401 ataaagttca ttttcagcaa gcggatgatg ataacgacgt ggcggtatcg ttggttattg
    17461 cgttaattgt ggaaaatccg cagcagcaat tgaaactttt acgctgttta tttggcaagt
    17521 tacaacagcc cgagatcgtc gagacactaa tcactcttcc tgaaacccag ttaaaggaat
    17581 acttcacaaa gtatgtttta gattcagacg aataaatccc tctgtaacaa taaaaaggac
    17641 tatttatgaa acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc tccacgatgg
    17701 cggcggaaga aattaaagag ttgtgtcaga gtcataatat tcctgttgaa ttaatccagt
    17761 gtcgggttaa tgaaatagaa acctatatgg atggcgtgca tttgatatgt actaccgcca
    17821 aagttgaccg tagttttggc gatattccgt tagttcacgg catgcctttt gtttctggtg
    17881 tcggtatcga agcattacaa aataaaattc tgactatctt acaggggtga cctatgtttt
    17941 cagaagtcat gcgttatatt ctcgacctcg gccctacggt gatgctgccg attgtcatca
    18001 ttattttttc taaaatatta ggcatgaagg caggcgattg ctttaaagcg ggtctgcata
    18061 tcgggattgg ctttgttggc attggccttg tgattggctt aatgctggat tccattggcc
    18121 cggcggcgaa agcgatggcg gaaaatttcg acctgaatct gcatgtggtc gatgtcggtt
    18181 ggccgggctc ttcaccaatg acctgggcgt cgcaaattgc gctggtggcg attccgattg
    18241 cgattctggt taacgtggcg atgctactga cccgtatgac gcgggtggta aatgttgata
    18301 tctggaatat ctggcatatg accttcaccg gcgcgttgct gcatctggca accggttcat
    18361 ggatgatagg gatggcgggt gtggtaattc acgcggcgtt tgtttataag ctcggcgact
    18421 ggtttgccca cgatacccga aatttctttg agctggaagg cattgctatt ccgcacggta
    18481 cgtcggcgta tctggggcca attgcggtgc tggtggatgc tatcatcgag aaaatcccag
    18541 gcgttaaccg tattaaattt agcgccgacg atattcagcg caaatttggc ccatttggcg
    18601 aacctgtcac cgtgggcttt gtgatggggc tgattatcgg catcctcgcg ggttacgatg
    18661 tcaaaggtgt attgcagctg gcggtaaaaa cggcggcagt tatgctgctg atgccacggg
    18721 tgattaaacc tatcatggat ggtttaacgc ccatcgctaa gcaggcccgt agtcgtttac
    18781 aggcgaagtt cggcggtcag gagttcctga ttggccttga tccggcgttg ctgctggggc
    18841 atacggcggt ggtatcggca agcctgattt ttatcccgct caccatttta attgctgttt
    18901 gtgttccggg taatcaggta ctgccgtttg gcgatcttgc caccatcggc ttctttgtgg
    18961 cgatggcagt tgccgtgcat cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca
    19021 ttatgagcat caccctgtgg atcgcgacgc aaactattgg tttgcacacc caactggcgg
    19081 ctaatgctgg ggcgttaaaa gccgggggta tggtggcttc aatggatcag ggcggttctc
    19141 ccattacctg gttactgatt caggttttct ccccgcaaaa tattcccggt ttcattatta
    19201 tcggcgcaat ttatctgacc ggtattttca tgacctggcg tagagcgcgc ggctttatta
    19261 aacaagagaa agccgttctc gcagaataat ttttacctga gggggaggta atccccctta
    19321 acaatcagga gtttttatga aatcagtggt gaatgatact gatggtatcg tgcgcattgc
    19381 agaaagcgtc attcctgaaa ttaaacatca ggatgaggta cgggtaaaaa ttgccagctc
    19441 gggcttatgt ggttccgatt tacccaggat atttaaaaat ggtgcacatt attatccaat
    19501 aacgttaggc catgaattta gcggctatat tgatgcggtg ggatccggtg ttgatgattt
    19561 acaccctggc gatgcggttg cctgtgtgcc gttattaccc tgttttactt gtccagagtg
    19621 tttgaaaggg ttttattccc agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg
    19681 tggatttgct gaatatattg tcgttaagcg aaaaaatgtc tttgctctac ccacggatat
    19741 gtctattgag gatggggctt ttattgagcc gattaccgtt ggcctgcatg cttttcattt
    19801 agcgcaaggt tgtgagaata aaaacgttat tattattggt gccggaacca ttggcctgct
    19861 ggctattcag tgcgctgtcg cgctgggagc aaagagtgtg acggctatcg acattagctc
    19921 agaaaaactg gcactggcaa aatctttcgg ggcgatgcta acatttaaca gccttgaaat
    19981 gagcgcgccg caaatgcagg gcgttttacg cgagctgcgc tttaatcagc ttatcctcga
    20041 gacggctggt gtcccgaaaa ccgtcgaact ggcggtagag attgccgggc ctcatgccca
    20101 gctggcgctg gtgggcacgt tgcatcagga tctgcattta acatcgacaa cgtttggcaa
    20161 aatattacgt aaagagctga cggttatcgg cagctggatg aactactcca gcccttggcc
    20221 ggggcaggag tgggaaacgg cgagccggtt gctgacagaa cgtaagttaa gcctggagcc
    20281 attaatcgct caccgtggaa gctttgaaag cttcgcccag gcggtgcgtg acatcgctcg
    20341 taatgctatg ccgggcaaag tgttgctcat tccctgaaac cgcgggccag cgtgatgctg
    20401 gcccggtatt gtgcaaaaca gatcattcac caatggtccc ccttcgttta cactagccac
    20461 aattgaatat gggtaaatga cgatgaattc attcgagcga aggaataaga tcatccaatt
    20521 agtgaatgaa cagggaaccg tgcttgttca ggatctggcg ggagtatttg ctgcctcgga
    20581 agcgacaatc cgtgccgatt tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca
    20641 tggcggtgcg gcgaaaataa tgtccggtaa tagtgaaacc gagacccagg aagttgggtt
    20701 taaagagcga tttcagctcg ccagcgcgcc aaaaaacaga atagcgcagg cggcagtcaa
    20761 aatgatccac gaagggatga ccgttattct cgacagcgga agtacgacaa tgcttatcgc
    20821 cgaaggatta atgaccgcca aaaatatcac ggtgattacc aacagtctcc cggcagcatt
    20881 tgccctttcc gaaaataaag acattactct ggtcgtctgt ggtgggaccg tccgccataa
    20941 aacacgctcg atgcatggtt ccattgccga gcgttcattg caggatatta atgccgattt
    21001 aatgtttgtc ggtgctgatg gtattgatgc ggttaatggt attacgacct ttaatgaagg
    21061 ttattcgatt agcggggcga tggttacagc cgcaaataaa gtgattgccg ttctcgattc
    21121 atcgaaattt aatcgtcgcg gttttaatca ggtactgcca atcgaaaaaa ttgacattat
    21181 tattaccgat gatgccgtgt cagaggtgga taaactggca ttgcagaaga cacgggtgaa
    21241 attaattacg gtgtagtatt gctctgtatg ccggataaag tttttacacc gcatccggca
    21301 ttacggattc tccctggttc aagagagaat agtgtgatta acgcaacaat gggcaatctg
    21361 gtggtaatcg acaacgcaac gccgccggaa gtatttcaat atgaaagttt tgtccgctca
    21421 acggttcgcc atcaagatta aaagtgattt cgtgtggggc ttgtatatca aaccacgacg
    21481 aagcgccttc gataatattc gggttatctt cgtcagattt taatgttgat acgagagccg
    21541 gaagaatttc atcgccggta aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt
    21601 tcgggcacaa ttgctgaccg ccaccggcct gacgcccgtt accaatacca atgaccaggg
    21661 cgtcaccttg ccagtgaaag ttttcaccgc ggatctcaca acggtccggt tgcagagtat
    21721 ccatgcgcat taagccatga atgatgtaag agacgccacc cagcgcggct tttaattttt
    21781 ccggcgtttc tgtggtaata cgcgtcccaa atccgcctgt cgccatatta ataaaacagg
    21841 tttgtttgtt gacctgcgcc atatctatcg caatggcgtc accggcaatt gccagtttca
    21901 gcgccttatc cagtgcctca ggaatcctta cactggtggc aaaatcattg gcggttccta
    21961 atggcaaaat tcctagcgcg ggtatgtcat ccccctcaca ctgaatcaac gccgtagaaa
    22021 cttcattaat ggtgccatcg ccaccaccgg caatcaccgt tgcgacgccc aacttccggg
    22081 cctcctctac atatcgtgcg gcatcgcctt tctcccaggt gacccgcaca tggatcgtca
    22141 ttccttcctc acgcaacagc ataattgctt cgcgcaatgg taaattgtca gtacttttgc
    22201 cattaagaat cagtaagctg gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg
    22261 gtctgtagat agtgtagagc agaaaagaaa aggtggagtc agaacaggat gaaagtcgga
    22321 ggataaaaaa tcagttcaag tatggaggat tcagtggtta ccaccaggag cttgcaatgg
    22381 ggacagtgaa tgttaacggt agccaccggt aaatttcaaa aaagagcata tacctaatat
    22441 tcaactaaac agtggcatct tcactatctt attctaaata tattaaagcc ctcatgggga
    22501 tacctgggca gggcttcatg tttgtaattc ctac
//
LOCUS       DARNVM010000068        22078 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.068, whole genome shotgun sequence.
ACCESSION   DARNVM010000068 DARNVM010000000
VERSION     DARNVM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.068"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..549)
                     /gene="paaY"
                     /locus_tag="SI621_002399"
     CDS             complement(<1..549)
                     /gene="paaY"
                     /locus_tag="SI621_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEI4065110.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGR"
     gene            complement(531..1481)
                     /gene="paaX"
                     /locus_tag="SI621_002400"
     CDS             complement(531..1481)
                     /gene="paaX"
                     /locus_tag="SI621_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HEI4065111.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(1582..2895)
                     /gene="paaF"
                     /locus_tag="SI621_002401"
     CDS             complement(1582..2895)
                     /gene="paaF"
                     /locus_tag="SI621_002401"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEI4065112.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPQDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(2922..4127)
                     /gene="paaJ"
                     /locus_tag="SI621_002402"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(2922..4127)
                     /gene="paaJ"
                     /locus_tag="SI621_002402"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HEI4065113.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4127..4549)
                     /gene="paaI"
                     /locus_tag="SI621_002403"
     CDS             complement(4127..4549)
                     /gene="paaI"
                     /locus_tag="SI621_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEI4065114.1"
                     /translation="MSNKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(4542..5966)
                     /gene="paaC"
                     /locus_tag="SI621_002404"
     CDS             complement(4542..5966)
                     /gene="paaC"
                     /locus_tag="SI621_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HEI4065115.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYE"
     gene            complement(5968..6756)
                     /gene="paaB"
                     /locus_tag="SI621_002405"
     CDS             complement(5968..6756)
                     /gene="paaB"
                     /locus_tag="SI621_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HEI4065116.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(6756..7523)
                     /locus_tag="SI621_002406"
     CDS             complement(6756..7523)
                     /locus_tag="SI621_002406"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HEI4065117.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNVLLTQLVNELEAAAID
                     PSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(7520..8590)
                     /gene="paaK"
                     /locus_tag="SI621_002407"
     CDS             complement(7520..8590)
                     /gene="paaK"
                     /locus_tag="SI621_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HEI4065118.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLNGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAEAALKALGMPEKAIHLERFNTAGTRVKRNVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(8598..9095)
                     /gene="paaD"
                     /locus_tag="SI621_002408"
     CDS             complement(8598..9095)
                     /gene="paaD"
                     /locus_tag="SI621_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HEI4065119.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9110..9856)
                     /gene="paaC"
                     /locus_tag="SI621_002409"
     CDS             complement(9110..9856)
                     /gene="paaC"
                     /locus_tag="SI621_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HEI4065120.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGFNEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMVAEMQYLQRVLPGQQW"
     gene            complement(9865..10152)
                     /gene="paaB"
                     /locus_tag="SI621_002410"
     CDS             complement(9865..10152)
                     /gene="paaB"
                     /locus_tag="SI621_002410"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HEI4065121.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(10164..11129)
                     /gene="paaA"
                     /locus_tag="SI621_002411"
     CDS             complement(10164..11129)
                     /gene="paaA"
                     /locus_tag="SI621_002411"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HEI4065122.1"
                     /translation="MNHENWNRKGDDVTQEERFEQRIAQETAIEPQDWMPDAYRKTLI
                     RQIGQHAHSEIVGMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAR
                     EDIYQKMLDGRMKYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAM
                     VKICKEESFHQRQGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSA
                     RSLAWKIKRFTNDELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEF
                     NEVINGRGICNQERLDAKRKAWEEGTWVREAALAHAQKQHACKVA"
     gene            11378..13423
                     /gene="paaZ"
                     /locus_tag="SI621_002412"
     CDS             11378..13423
                     /gene="paaZ"
                     /locus_tag="SI621_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEI4065123.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGGRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQVLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWSVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            13671..15944
                     /gene="tynA"
                     /locus_tag="SI621_002413"
     CDS             13671..15944
                     /gene="tynA"
                     /locus_tag="SI621_002413"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEI4065124.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDTKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSSHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGAIKKDK"
     gene            complement(16003..17502)
                     /gene="feaB"
                     /locus_tag="SI621_002414"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(16003..17502)
                     /gene="feaB"
                     /locus_tag="SI621_002414"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HEI4065125.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTVDANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHGEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGSVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVRSFLDDAQAQQAELIRGASGPAGEGYYVAPTLVVN
                     PNAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            17738..18643
                     /gene="feaR"
                     /locus_tag="SI621_002415"
     CDS             17738..18643
                     /gene="feaR"
                     /locus_tag="SI621_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HEI4065126.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(18815..19141)
                     /locus_tag="SI621_002416"
     CDS             complement(18815..19141)
                     /locus_tag="SI621_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEI4065127.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVNDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGING
                     KWVRKF"
     gene            complement(19149..19334)
                     /locus_tag="SI621_002417"
     CDS             complement(19149..19334)
                     /locus_tag="SI621_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEI4065128.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(19331..21970)
                     /locus_tag="SI621_002418"
     CDS             complement(19331..21970)
                     /locus_tag="SI621_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HEI4065129.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVEFDSACLAKL
                     PQTEQSLAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLSQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFIPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVVMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT         5341 a         6031 c         5357 g         5349 t
ORIGIN      
        1 cctgccaggt tcaacttccc gcaatggctc gacttgatgt aacgtctgct tacagcgtgt
       61 caccagcacc tgatactcat gcgtgccctg ctttttccat gccagttcct gctcactaag
      121 ttcacgaatc gcttttgccg ggctgccgac aatcaggtaa ttagcaggca tttctgcttt
      181 ggctttcaca aatgcggaag cgccaacaat gctgttctcg ccaatcaccg caccgtccat
      241 cactaccgcg ttcataccca ctaatgcatt gcggcggata atgcagccgt gaaggatagc
      301 gctatgacca atatgcccat cttcttccac gacagtatcc tgatcgggaa aaccgtgcat
      361 aacgcaatta tcctgaatgt tcgcgccatc tttcaccacg atacgaccga aatcgccacg
      421 caggctggca tttggcccaa cgtacacgcc cttgccgaga ataacatcgc cgatcaatac
      481 cgctgtcgga tgaacaaaac tctcttctgg tacaaccgga gtcagaccgt ctatctggta
      541 aattggcata acgcctcctg ttcaatgttc aaaccgccaa aacgttgaaa atacaggctt
      601 cccggcgcag gcagttcacc gaccgaggtt tcaccttttt cactaacgaa cgctaaagca
      661 gcaggcgcta cgcgctgata aatgttgata cacagctgac gcgccgtatg ccctgcccag
      721 tgagccggaa gcaactcctc cggcaacaat gggtctttaa ggacgacacg gcgataaaaa
      781 tggatcagta aaagctgaat atgaaatgca cgctccgggg ttaattcgtc tgccgcctct
      841 tttaacagcg gcaccagcgg gcggaatgac tgaataaagg tttcgtacat ggcattttgt
      901 tcagttaaat gccagcactc ttctactctg gcacgcagtg ctgcgcgaga aagcgccagt
      961 ggtatttgcg cttcaaaaca aatcacgtta tccgccacac ccgcttcatg caaaagtgtc
     1021 tgtacatcgg ccagtttttg cgacggcgat gccatcagac tgggtgccag tgcgccaaaa
     1081 ccttgccaga tcaactgctt tttgacatca gccagcgtcg ctttatctaa accttccgag
     1141 agcaacagga gccatttacc atcccatgca ggttgctctg cgcgataaat tttactttct
     1201 gcccgtcgcg tcaggcgcaa gcctttatca ctgaggctat agaaactgcg tcgcccgatg
     1261 cgggaaacat ccagccagcc ttctttatta agacgaaaca aagcggtgcg gacgaaacgc
     1321 tcaccaaatc ccagcccttc cagcaaagca gccagactac ccagccagat ttcaccacca
     1381 cgatgggaaa gcgaatcacc atacagagag gagatcaaag atgtgccact gacaggaaca
     1441 gcgtttacag catgttggat aaaagtatca agtttactca ttcgattcag tctgttgtta
     1501 ttgttgtgat ttatgaatca tagcacaggt cgtatcgact tctggccctg gcgctttacc
     1561 tcaccagggc cagacgacac atcaggcacc aacaatattg cgcagatcaa acacccgaca
     1621 cgccttgcct tctgaacgcg ggatactgcc acagttcacg atcattacat cagtagagat
     1681 ccccaccatc gacttaatcc gatggcgtag ctggtggcac acctggcaac gctgctcatg
     1741 cgtcaatgtc aggctgcttt ctttcaactc cactttcaca gaaagtgaat caagatgccc
     1801 ccggcggttc acctccagtt ggtaatgcgg cgataaatgt tcgaatttga caatctcttc
     1861 ttccagttgt gacggaaaga catttacacc gcgaataatc aacatgtcat cgctgcgtcc
     1921 gctgatgcga tccatgcggc gcatagtccg cgccgttcct ggtaacagtc gggttaaatc
     1981 acgcgtgcgg taacgaatga ccggcaatgc ttctttggtc agcgtggtga ataacagttc
     2041 gccatgttca ccatcggcaa gcggtgtgcc gtcctgagga ttaacaattt caggatagaa
     2101 atgatcttcc caaatggttg ggccgtcggt agtttccaga cactccatcg ccacccccgg
     2161 ccccatcact tctgacaggc cataaatatc cagtgcggtg atccccaggc gacgctcaat
     2221 ttctttacgc atggcctgtg tccacggctc agcaccaaat actccaaccc gcagcgagca
     2281 accgctggca tcaccgccca actgccgctc cagctcttca atcaggttaa ggcaataaga
     2341 tggcgtaacc atgatcatat ctggttgaaa atcacggatc agttgcgctt gtttttccgt
     2401 ctgcccgcca gacatcggga tcaccgtagc gcccagacgt tcggcaccat agtgcgcacc
     2461 cagcccgcca gtaaacaggc cgtaaccata ggcgacatga attttgtctt tgggcgagcc
     2521 ccctgccgca cgcaaagaac gcgctacaat attggcccag ttatcaatat cgttttgcgt
     2581 atagccgaca actgtcggtt tacctgtggt tcccgaagaa gcatgaatgc gcaccacttg
     2641 ttccattggc acggcaaagg tgtcaaaggg atagttgtcg cgcagatcct gtttggtggt
     2701 acagggaaat ttacgcaggt ctgaaagttc cctgaaatca tcaggatgta cgcctgctgc
     2761 gtcgaatttg cgccgataca tcggcacatt ttcatacgcg tgtttgagcg tccatttcag
     2821 acgctgtgtt tgcaacgcct gtaactcatc aacagacgcg gtttcaatcg ggtcaagctt
     2881 tgtattggtt atcattgtag gttactcgca ggttgatatg ctcaaacacg ctccagaatc
     2941 atggcgatgc cctgaccgac accgatgcac atggtgcata atgcataacg accgttacgc
     3001 cgatgcagct catggctggc agccagtgcc aggcgggcac cactcattcc caatggatgg
     3061 cctaaggcga tagcgcctcc gttggggtta acatgtgggg catcatcagg cagccccaat
     3121 tcgcgtagta cacccaacgc ctgggccgcg aacgcttcgt tcagttcaat cacgtccata
     3181 tcgtgaatac tcaaccccgc gcgttccagc acccgacgag ttgcaggcac cggaccaagc
     3241 cccatcaggc gcggttccac cccggcggtt gccatggcta cgatacgcgc tcgcggtgtc
     3301 agtccttgcg ctgctgccat ctgttcactg gcaataatca acgcagcggc tccatcattc
     3361 accccggagg cattgcctgc ggtgatcact ccattggcac gaaatggtgc ttttagtcca
     3421 cgtaactgtt ccagcgtcgt ttccgggcgc agatgctcgt catgttgtat ttctgttaca
     3481 acaccttttt tgtttttcaa cacaaccgga acaatctctt cagctaaaat gcctgaagat
     3541 tgcgcttttg ctgtacgttg ctgactgcgt agcgcaaaac tatcttgatc ttctcgcgag
     3601 atttttaaca gttcagctac attctctgcc gtttccggca tgctgtcagt tccaaattgt
     3661 tgagccatga gcgggttcac aaatcgccag ccaatagtgg tatcaaacat ctcagcctga
     3721 cgagaaaatg cactggttgc cttgcccata acaaacggtg cccgagacat tgactccacg
     3781 ccaccagcga tcagcaaatc gccatcgccc gctttaatcg cccgtgcggc gaaccccagt
     3841 gcgtccagtc cggaaccaca caagcggtta atggttgtgc cggaaacact ctgcggcagc
     3901 cccgccagta aagtcgccat ccgggctacg ttacggttat cttctcccgc ctgattagca
     3961 cagccgagga tcacatcatc gatacattcc gcatcgagac gcgggtttcg caccagcagt
     4021 tcccgcaaag ggatagcagc cagatcatca gcccgaacac ctgataatgc cccgccgtag
     4081 cgaccaattg gcgtacgaat tccgtcacag ataaaggctt cacgcatcag gcttctcctg
     4141 taatggtgcc gccgatgcgg tgagatttac cgcgaaacag cgcaaccgtt ttttgttgtt
     4201 ggttaacaat ttcgatgtcg taaacaccgg tttgcttgcc ctgatgacgt acctgcgcag
     4261 tagcagttaa ggtgtctccg gcaaagcctg gacgcaaaaa atcaatcgtg caggcagaag
     4321 cgacggctgc cagcccctgg ctattgcagg cgtaggcaaa ggcagtatca gccagtgaaa
     4381 atagctgccc gccgtggcaa ctttgatgac cattaagcat ttgtgcagtg acggtcatgg
     4441 tcactacagc aaagccttca tccattgaga taatgtcgat gccaagcgct ttggcgcagg
     4501 catcgttctc atacattgca tgggcatttt gccaggcctt attactcata accgctctcc
     4561 agaagcgctc gttggcgcag caatgaacat gggcgatagc gttcttcgcc atagtgatgt
     4621 tgtagatttt ccaggagcct taatattcgc tgccagccaa gttgcgctcc ccaggcaagc
     4681 gggccatatg gataattcac cccaagacgc atggcggtat cgatatcctg ttcagaggcc
     4741 acgccttttt gaagcgcatc aagggcttca ttgatgatca ttgctaccgt tcgccaaatc
     4801 agcattcctg ggtaatctgc aatttgcagc actgttttgc cctgctgttg caggtaataa
     4861 atggccttgc gggtcgctga gtccgggttc accgctgccg cagcaatggt caccaccttg
     4921 cccgccattt tgtcgaccac taccaccggg cgtgccagtc gtattgccag cgcctgtgcc
     4981 gtctcgcctt gtgtctcaat caataaaaca tcgtcaattt ccgtgacacc gtcacttttc
     5041 ttttctactt tcatggggct aaaactgtcg cttaccgctt ccaggccaac aactgcctca
     5101 cgttccgcgc gccagtcgta cacgcccagc ccacttttct tgcccaaccg tccaccaatc
     5161 accagttcct gttgcaccag cgaaggtaaa aaacgacgct cctgccagaa agcgttaaac
     5221 accgaacagg tgacagcaaa attgacgtcc tgaccaatca gatcggttaa ttccagcggc
     5281 cccatcggga aaccagcgcc atcgcgaagt gcagcgtcaa tcacttctgg tgcagcaacc
     5341 tgctcttcca gtgcccgcca ggcctcggaa taataaggac gcgcaacacg gttaacgata
     5401 aatccaggag tcgaatgaca gcgcacaggc tgcttacccc aactcaacgt tagttcacac
     5461 aactgctcaa caacttccgc tgccgttgcc agcccactga ccacctccac caacttcatc
     5521 accggtgccg ggttaaaaaa atgcagcccc gcaacacgtt caggattttt tacctccgca
     5581 gcaatcgcgg ttatagagat tgacgaagtg ttagtggtca atagcgtttg tggcgggcaa
     5641 acttccgcca gctgtgcaaa gagcgctttt ttgacttcca gacgttcaga cgccgcttca
     5701 atgaccaggt ccgcagctgc cagcgcgtga atatcggtca ccgggatcag gcgtttcaat
     5761 gtgcgttcac aggtttcagc agtcagtttt ccccgcgtca ctcgtgaatt tagccgcgcg
     5821 tgtatcccgt cgattgcgcg ggtcagcgct tcagcagaaa tgtcatacag taaaacctga
     5881 tgtccatgac tggcggcaac ttcagcaatg ccagccccca tggtgccgct cccaatcact
     5941 gccacagttt gcacatttat catcatgcta tttccccgtg aactgcggtg agcgtttagc
     6001 caggaacgca ctgacacctt cacgataatc tgcgctccgt ccggcaagtc gctgatagtc
     6061 acgttccaga tccagttgcg tatcaagcgt attggtttca gcgctattta tcgcttgctt
     6121 gataagtcca agaccaaatg tcggttgtgt cgccagatgc cgtgccagct gttgcgcggt
     6181 atctgccagc gtttcatcat caacaacctg ccagatcatc ccccattcgt gcgcctgttc
     6241 agcactcagt tgattcccca gcagtgccag ccccatggcg cgcgctcgtc cggcaacgcg
     6301 tggcagtaac caggtaccac cgcaatcggg taccaaacct aacttactaa acgccatgac
     6361 gaattttgct gaacgggcag caataacgat atcgcacccc agtgccagtg ttgcgcctgc
     6421 ccctgcagcc acgccattaa ctgcacagat caccggtttt ggcagttttg ccaggcgacg
     6481 taccagtggg ttatagaaac gctccactga catacctaaa tccggtgcgg ggcctgtggg
     6541 atcaacgtta cgatcgttaa gatcctgacc agcacaaaat ccacgtccgg cacctgtaat
     6601 taacaagcaa cggatagtgt cgtcgcgctc gacctgtttc aggcactctg ccagttgtgc
     6661 gtgcatctca tcattaaaac tgttcaggcg ttccgggcgg ttgagcgtta gtgtcatcac
     6721 gcccttttct acatgactga ggatgaattc catcattagc gtcctttaaa gtcgggcgtg
     6781 cgtttttgta agaaagcgga gatgccttca tgacgatctt ctgttgccgc cagcaaggtg
     6841 aataactgtc gctcctgggc aagtccggct tgcaaagcca cttcctgcga ctggcgcagc
     6901 gcttgctttg ccgcttgtaa ggccagcggc gagtgacgtg ccattttcga cgccagctgt
     6961 aaggcgtatt cgagggttaa atcgctgggg aagacgtcgc taaccagccc ggcctgctgt
     7021 gcttgctgag cggtgatact ttctccgctc agcaccattt tgctggctaa cgatttaccg
     7081 acactacgga ttaaacgttg cgttccgcct gcgccaggca tgatgccgag agtgatttcc
     7141 ggcaacccaa aacgcgcgtt ctctccggca accaccacat cgcacaacaa tgccagttcg
     7201 caacccgcac caagcgcgta accgttgacg gctgcgatga gtggtttgtt aaaggcctgc
     7261 aatcgcgccc atagctgcgg acgtgtatcg tttaaggtgg ccgcgagatc tttttctgcc
     7321 atttcgttga gatcggcccc agcggcaaaa aagcgtgcat taccggtaat cacacagacc
     7381 gaaatgctgg gatcgatagc cgcagcttcc agctcattta ccagttgcgt cagcaggaca
     7441 ttattcagcg cattacgtgc ggcaggacgg ttaagggtca gcaacaatac tcgttgctga
     7501 cggctgacga tcagttcgct catgccatcc ccttcgcgtc aaagtcaacc accacatcgc
     7561 tggtcagcgg cagtgcctgg caactcaaca catagcctgc agccagttca tccggttcca
     7621 gactgtaatt ggtttccatc gccactttgc cacgcagcac tttgcatttg caggtcgcac
     7681 agacgccgcc tttgcaggca tagggcagat cagccccctg gcgcaatgcc gcatcgagaa
     7741 tgctttcatc gtcggcatta agcacgattt cccgatcccg cccatcctga cgcacagtca
     7801 ctttttgtcc gtcactttgc acgttaacgt tacgtttgac gcgcgtgcca gccgtattaa
     7861 accgctccag atgaatcgct ttttctggca ttcccagtgc ttttaaggca gcttccgcgt
     7921 catccatcat cgccgccgga ccacaaataa acgcctcatc ataaagacga aaattaatga
     7981 gcgaggcccc aagtgactgt aatttttcac cgtcaatacg cccgtgaagc agatcgctgt
     8041 cgagagtttc ctgactgaaa atgcacaaca actgtaaacg ctgaggatat ttgtctttca
     8101 ggtctgccag tgcctggcga aacatcatgc tctggctggt acggttaccg taaatcagag
     8161 tgaactgact ttcaggctcg gtttgtaaag tggtggcgat aatcgccagc attggcgtaa
     8221 tacccgatcc tgctgcaata gccagatagc ggccctggcg ttcggcctgt ggctgatagc
     8281 cgaaatgccc ctgcggcacc atgacttcca gcgtcatccc ctggcggatg tgttcgcggg
     8341 catagcggga gaaacgtccg ccttcaatgg ctttcaccgc cacactaatt tcgccaggca
     8401 ggtagctacg gcaaatggag taacaacggc gtaattcttc accattaagg ctggctttta
     8461 aggtcaaatg ttgaccggga cgaaagcgat acgcctcctg caaaggctgg ggtaccgcaa
     8521 aggtaatggt taccgcatca cgggtttctg gctccacttt tgccaccgtt aaggaatgaa
     8581 acgttgtcat ggcatcctca aatacatttg aaataatcga aaggttcgcg acagctatcg
     8641 cagcgataca atgctttgca ggccgtggaa ccaaattcac tgataagtgt ggtatggacg
     8701 ctggcgcaac gcgggcaacg tacttctggc ggcaaatggg catggcaact gtgtccggcg
     8761 ggcgggctaa tgccatactg tcgcagacgc tcacgggcat cgggggtcat ccagtcggtg
     8821 gtccacgccg ggtcgagttg cagcacaacc tgaacggggg taaacccgtt ggttgtcatt
     8881 gcctcacgta tcgcgccaat caaatgttcc gttgccggac aaccggaata tgtcggcgta
     8941 aagccgatca cccatccttc tcccatctgt gtcacattac gcaccatgcc taaatcagta
     9001 atggtcagca ccgggatctc cggatccggg atctggctga gtagcgccca tatctcatgc
     9061 acctgtggcg gtgcaatggt ggccagacgt tgcataccca tctcctctgt taccattgct
     9121 gaccgggtaa gacacgctgg agatactgca tttctgccac catgggtcca aggtgttcgg
     9181 tatgcagtcc ttttttaccg ccagtgcgat acgcctgctc ttgcggtacg ttcaatgtgg
     9241 cttcgttgaa cccagcaaaa acttcggctt cccacgctgc gcgtaaagtg cgtgggtcaa
     9301 ccgcaatacc ctcttcactc agtgcaatat caatctcgtc ggcatcgaac agttcggcgg
     9361 taaaacgcca caacttgttg atggcctgct gcatcttttg ccctgatacg tcagtaccat
     9421 tgcccagtcg ctccagccag ccacgactaa aacgcaggtg atagcgagct tctttaattg
     9481 ctttggcaga aatcgccgcc agttgcggct cgcggctttc catcagacgg gtaaagagcg
     9541 ccacatgcca tgcatcgatg aaatactgac gtgcaatggt gtcggcaaaa ttgccgtttg
     9601 gttgttcaac caacaataag ttgctgaact ggcgctcgtc tcgggtgaag gccagggtat
     9661 cttcatcgcc ttctcccgct aattcagcgg catacgataa gaagttgcgt gcctgaccta
     9721 ataaatcgag gccaatgttt gccagtgcga gatcgatttc cagttccggt gcgtgaccgc
     9781 accattcacc caaccgctgg gagagcacca ggcagttatc gcccaggcgc aaggtgtaag
     9841 ccgttaactg attcatttcc gacctcacat gtgctcaatg ccatcaggga tggtgtaaaa
     9901 cgttggatgg cgatagacct tgctttcagc cggatcaaaa aattcaccgc gttcttccgg
     9961 ttgcgaggca acaatttcac tcgccttcac cacccaaatt gaacatcctt cgctacgacg
    10021 ggtataagca tcacgggcat tttccagtgc catccgctca tcggcagcat gtaaactgcc
    10081 aacatggcgg tgtgataagc cctgtttgcc acgcacgaaa acttcgtata acggccagta
    10141 aacattactc attttgaaac tccttatgcg accttacagg catgttgttt ttgtgcatgg
    10201 gccagcgcag cttcccgtac ccaggtacct tcttcccagg ctttacgttt ggcgtcgagc
    10261 cgctcctgat tacaaattcc gcgaccgttt atcacttcgt taaactcctg ccagtcgatc
    10321 tcaccaaagc ggtagtgacc gctttcagta tcaaaatgca gatccgggtc aggaacggtc
    10381 ataccgagca tttcaacctg tggaacggtg ttatccacga aacgctggcg gagttcgtcg
    10441 ttggtgaaac gtttgatttt ccaggcgaga cttctggcgc tgtttggcga gttatcatcg
    10501 tttggcccga acatcattaa ggctggccac cagaaacggt taatggcgtc ttgcaacatc
    10561 tgcttttgag cttcgctgcc ctgcgccagt gccatgcagg cttcaaaccc ctgacgctgg
    10621 tgaaaactct cttctttgca gattttcacc attgctctgg catacgggcc ataagaagtg
    10681 cggcataacg ccacctgatt gacgatcgct gcgccatcca ccagccagcc gataacaccg
    10741 atatcagccc aactcaatgt cggataatta aagatggagg aatatttcat ccgtccgtcg
    10801 agcatttttt ggtagatgtc ttctctggcg cagcccagtg tttcggcggc gctatagaga
    10861 tacagaccat gacctgcttc atcctgcact ttggccaaca gaatggcttt acgccgtaag
    10921 gttggtgcgc gagtgatcca gttaccttca ggcaacatac caacaatctc ggagtgcgca
    10981 tgctgcccaa tctggcggat caatgtcttt cggtaagcat cgggcatcca gtcctgcggc
    11041 tcgatagccg tttcctgggc tatccgttgc tcaaagcgtt cttcttgggt cacgtcatca
    11101 cctttacgat tccagttttc gtgattcaat caaaaacacg aatcacattg ttttatgaaa
    11161 gttacacagt ggttaactaa aatcacaaat tttgtatatt tgaactgtga cggatttcgc
    11221 accctatttt tataaatcat tatttatcag tgtattaatg tttttcgcag ttgctataca
    11281 gatcgcacag ttaacatttc gttaaaagac cacttgcttt ttatgattcg cgatttaact
    11341 attagcaacg gaaatgtgaa acatctggag agtagcgatg cagcagttag ccagtttctt
    11401 atccggtacc tggcagtctg gccggggccg tagccgtttg attcaccacg ctattagcgg
    11461 cgaggcgtta tgggaagtga ccagtgaagg tcttgatatg gcggctgccc gccagtttgc
    11521 cattgaaaaa ggtgcccccg ccctccgcgc gatgaccttt atcgaacgtg cggcgatgct
    11581 taaagcggtc gctaaacatc tgctgagtga aaaagagcgt ttctatgctc tttctgcaca
    11641 aacaggcgca acgcgggcag atagttgggt tgatattgaa ggcggtattg ggacgttatt
    11701 tacttacgcc agcctcggtg gccgggagct gcctgacgat acgctgtggc cggaagatga
    11761 attgatcccc ttatcgaaag aaggtggatt tgccgcgcgc catgtactga cctcgaagtc
    11821 aggtgtggcg gtgcatatta acgcctttaa cttcccctgc tggggaatgc tggaaaagct
    11881 ggcaccaacg tggctgggcg gaatgccagc catcataaaa ccagctaccg cgacggccca
    11941 actgactcag gcgatggtga aatcaattgt cgatagtggt cttgttcccg aaggcgcaat
    12001 tagtctgatc tgcggtagtg cgggcgacct tttggatcat ctggacagcc aggatgtggt
    12061 gactttcacg gggtccgcga cgaccggaca gatgctgcga gttcagccaa atatcgttgc
    12121 caaatctatc cccttcacta tggaagctga ttccctgaac tgctgcgtac tgggcgaaga
    12181 tgtcacccca gatcaaccgg agtttgcgct gtttattcgt gaagttgtgc gtgagatgac
    12241 cacaaaagcc gggcaaaaat gtacggcaat ccggcggatt attgtgccgc aagtattggt
    12301 taatgctgtc agtgacgctc tggttgcgcg attacagaaa gtcgtggtcg gtgatcctgc
    12361 gcaggaaggc gtgaaaatgg gcgcactggt aaatgctgag cagcgtgccg atgtgcagga
    12421 aaaagtgaac acattgctgg ctgcaggatg cgagattcgc ctcggtggtc aggcggattt
    12481 atctgctgcg ggtgccttct tcccgccaac cttattgtac tgtccgcagc cggatgaaac
    12541 accggcggta catgcaacag aagcctttgg ccctgtcgca acgctaatgc cagcacaaaa
    12601 ccaacaacat gctctgcaac tggcttgtgc aggcggcggt agccttgcgg gaacgctggt
    12661 gacggctgat ccgcaaatag cgcgtcagtt tattgccgac gcggcacgta cgcatgggcg
    12721 aattcagatc ctcaatgaag aatcggcaaa agaatccacc gggcatggct ccccactgcc
    12781 acaactggta catggtgggc ctggtcgcgc aggaggcggt gaagaattag gtggtttacg
    12841 ggcggtgaaa cattacatgc agcgaaccgc tgttcagggt agtccgacga tgcttgccgc
    12901 tatcagtaaa cagtgggtgc gtggtgcgaa agtcgaagaa gatcgtattc atccgttccg
    12961 caaatatttt gaggagctgc aaccaggcga cagcctgttg actccccgcc gcacaatgac
    13021 agaggccgat attgtgaact ttgcttgcct cagcggcgat catttctatg cacatatgga
    13081 taagattgct gctgccgaat ctatttttgg tgagcgtgta gtacatgggt attttgtgct
    13141 ttctgcggct gcgggtctgt ttgtcgatgc cggagttggt ccggtcattg ctaactacgg
    13201 gctggaaaac ttgcgtttta tcgaacccgt aaaaccaggc gataccatcc aggtgcgtct
    13261 cacctgtaag cgcaagacgc tgaaaaaaca gcgtagcgca gaagaaaaac caacaggtgt
    13321 agtagaatgg tcggtagagg tattcaatca gcatcaaacc ccggtggcgc tgtattcaat
    13381 tctgacgctg gtggccaggc agcacggtga ttttgtcgat taatcggtca atgaagggca
    13441 acggcgaata gttgcccttt tatttcacca aattttgtga cgttgtcaca ttatgcatga
    13501 tgtgtatctg tattttcagg gcactcactg tatgaaaagc tggcacacct gccaaacaac
    13561 ctggcaggtg caggcaatcc cctttacatc agtactgata atgtgaacct gactaaaccg
    13621 cccacagagc gcggttgcta acaagaacac aacatctgac gaggttaata atgggaagcc
    13681 cctctctgta ttctgcccgt aaaacagccc tggcgttggc agtcgcctta agtttcgcct
    13741 ggcaagcgcc ggtatttgcc cacggtggtg aagcgcatat ggtgccaatg gataaaacgc
    13801 ttaaagaatt tggtgccgat gtgcagtggg acgactacgc ccagatcttt accctgatta
    13861 aagatggcgc gtacgtgaaa gtgaagcctg gtgcgcaaac agcaattgtt aatggtcagc
    13921 ctctggcact gcaagtcccg gtagtgatga aagacaataa agcctgggtt tctgacacct
    13981 ttattaacga tgttttccag tccgggctgg atcaaacctt tcaggtagaa aagcgccctc
    14041 acccacttaa tgcgctaact gcggacgaaa ttaaacaggc cgttgaaatt gttaaagctt
    14101 ccgcggactt caaacccaat acccgtttta ctgagatctc cctgctaccg ccagataaag
    14161 aggctgtctg ggcgtttgcg ctggaaaaca aaccggttga ccagccgcgc aaagccgacg
    14221 tcattatgct cgacggcaaa catatcatcg aagcggtagt ggatctgcaa aacaacaaat
    14281 tgctatcctg gcaacccatt aaagacgccc acggaatggt gttgctggat gatttcgcca
    14341 gtgtgcagaa cattattaac aacagtgaag aatttgccgc tgccgtgaag aagcgcggta
    14401 ttactgatac gaagaaagtg attaccacgc cgctgaccgt aggttatttc gatggtaaag
    14461 atggcctgaa acaagatgcc cggttgctca aagtcatcag ctatcttgat gtcggtgatg
    14521 gcaactactg ggcacatccc atcgaaaacc tggtggcggt cgttgattta gaacagaaaa
    14581 aaatcgttaa gattgaagaa ggtccggtag ttccggtgcc aatgaccgca cgcccatttg
    14641 atggccgtga ccgtgttgct ccggcagtta agcctatgca aatcatcgag cctgaaggta
    14701 aaaattacac cattactggc gatatgattc actggcgtaa ctgggatttt cacctcagca
    14761 tgaattctcg cgtcgggccg atgatctcca ccgtgactta taacgacaat ggcaccaaac
    14821 gcaaagtcat gtacgaaggt tctctcggcg gcatgattgt accttacggt gatcctgata
    14881 ttggctggta ctttaaagcg tatctggact ctggtgacta cggtatgggc acgctaacct
    14941 cgccaattgc ccgtggtaaa gatgcaccgt ctaacgcagt gctccttaat gaaaccattg
    15001 ccgactacac tggcgtgccg atggagatcc ctcgcgctat cgcggtattt gaacgttatg
    15061 ccgggcctga gtataagcat caggaaatgg gccagcccaa cgtcagtacc gaacgccggg
    15121 agttggtggt gcgctggatc agtacagtgg gtaactatga ctacattttt gactggatct
    15181 tccatgaaaa cggcactatt ggcattgatg ccggtgctac gggcatcgaa gcggtgaaag
    15241 gtgttaaagc gaaaaccatg cacgatgaga cggcgaaaga tgacacgcgc tacggcacgc
    15301 ttatcgatca caatatcgtg ggtactacac accagcatat ttataatttc cgcctcgatc
    15361 tggatgtaga tggcgagaat aacagcctgg tggcgatgga cccggtggta aaaccgaata
    15421 ctgccggtgg cccacgcacc agtaccatgc aagttaatca gtacaacatc ggcaatgaac
    15481 aggatgccgc gcagaagttt gatccgggca cgattcgtct gttgagtaac ccgaacaaag
    15541 agaaccgcat gggcaatcca gtttcctatc aaattattcc ttatgcaggt ggtactcacc
    15601 cggtagcaaa aggtgcccag ttcgccccgg acgagtggat ctatcatcgt ttaagcttta
    15661 tggacaagca gctttgggta acgcgttatc accctggcga gcgtttcccg gaaggcaaat
    15721 atccgaaccg ttcctctcat gacaccggtc ttggacaata cagtaaggat aacgagtcgc
    15781 tggacaacac cgacgccgtt atctggatga ctaccggcac cacacatgtg gcccgtgccg
    15841 aagagtggcc gattatgccg accgaatggg tacatactct gcttaaacca tggaacttct
    15901 ttgacgaaac gccaacgcta ggggcgataa agaaagataa gtgattgttt cagactaaaa
    15961 aaacggcacc tggtgccgtt ttttacttat gcgcgaacca gattaatacc gtacacacac
    16021 cgacttggtt tcacaccaac cgtccagcca gtccgggcca aaatcacggc ctgttcctga
    16081 ctgcttcatc ccaccaaacg gtaagttagc gtcaattaag gtatgactgt ttacccacac
    16141 cgtacctgcc tgtaagcgat cgctatattc cagagcctgg gagagatttt gcgtccagac
    16201 gctggcagtt aagccatatt ccgtgtcgtt tgccagttgt aacgcctctt ctccatccgc
    16261 tacacgcacc aggttaacca ccggaccaaa cacctcttca cgagttaagc gcaatttagc
    16321 attgggattt accaccagcg ttggcgcaac ataatatccc tctccggcag gtccactcgc
    16381 cccgcgaatc agctctgctt gctgtgcctg cgcatcgtca aggaatgaac gcactttgtc
    16441 gcaatgcgca cgagaaacca aagggttaat ctgtgcaaca ggtgacatcc ccggtcccac
    16501 ttgcaacgat tttaccgcct gctcaaatcc actaaccagc gtgtcaaaca acggcgcttc
    16561 aatataaatt cgcgaactgg cggcgcatac ttgcccctga ttcaggaaac ttccggtcat
    16621 caagccttca ataacccatt gcggatcagc atcttttaat acaattgccg ggtttttacc
    16681 gcccagttcc agcgttacac gcgtcaagcg atcagcagca gttctggcaa tacctttgcc
    16741 cgtcgcggtt gaaccggtaa aactgatttt cgcaacatga ggatgtgacg tcagggccgc
    16801 gccgcataca gaacctgacc cggtgaccac attaaaaacg ccatcaggaa tacctgcctc
    16861 gctggccagt tccgccacgc gcaacatagt cagtggcgtg gtttccgatg gcttaatcac
    16921 gattgaacag cctgctgcca gtgctggcat caccttccac atgccaatca tcaacggaaa
    16981 gttccatggc acaattcccg ctaccacgcc aaccggctct ttacgcgtcc aggcctgata
    17041 acgcgccccc tggggtaagg gaatcgacaa gtccagcgtt ttacccgcga ttttggtcgt
    17101 taacccggcg gtataacgca tccagttcag cgtacagccc acttcaaaag cacgggaaat
    17161 agcaattgac ttaccttgct ccagggtttc cagctgtgcc agctcctcac cgtgctgttc
    17221 caccagatcg gcaaaacgta gcaggatacg ttcacgctct gcgggtaatc gcccggccca
    17281 gcgacgcgag acaaaggccc gccaggcaga catgaccgcg ttatctacat ccgcttcgtt
    17341 ggcatcaaca gttgacgcaa tttcttgccc ggtggccgga tcaaagatcg ccaaccgttt
    17401 ttcactttgt gcggggccag gacgaccatc aatataaaga ccgtgttgac gatcgagaaa
    17461 ctgttggacc tggcttaata ctgctacatg cggctctgtc atcacttttc cttattattt
    17521 acccagtgtg atttcagtgt acgcagcgac aagaaaagtc acttttttcg ctgtgacatt
    17581 cgctttgtca gctgtgacaa gctccgcaaa tcgtgacaat aaccgcttat gtctctatgc
    17641 ctgacaagac ttatttccta tagtcaggct cattagcttc gtttattgca acacaaatgc
    17701 aacaataaaa atacatttca cagagcgaaa acgtgccatg aaccccgcag tggataatga
    17761 gtttcagcaa tggctttccc aaatcaatca ggtatgcgga aattttaccg gacgcctgct
    17821 gactgagcgt tacacgggtg tactggacac gcattttgcc aaaggactaa agctgagtac
    17881 cgtgacaacc agcggggtga atttatcccg cacctggcag gaagtaaaag gcagcgacga
    17941 tgcctggttt tacaccgttt ttcagcttag tggtcaggca ataatggagc aggatgagcg
    18001 tcaggtgcag attggcgctg gcgatattac gttactcgat gcctcacgcc cctgttcgct
    18061 ttactggcag gagtcttcta aacagatttc attactttta ccacgcactc tgctggaaca
    18121 atattttccc catcaaaaac ctgtctgcgc agaaagactg gacgctgact tacctatggt
    18181 gcaactcagt catcgcctgt tgcaggagag catgaataac ccggcacttt ctgaaacaga
    18241 aagtgaagct gcgctacagg cgatggtgtg tctgcttcgc ccggtacttc atcagcggga
    18301 atctgttcaa cctcgccgtg aacgtcagtt tcaaaaagtg gttacgttga tagacgataa
    18361 tattcgcgaa gagatattac gcccggagtg gatagccgga gagacaggta tgtcagtacg
    18421 tagtttgtac cgaatgtttg ccgataaagg tttggtggtc gcgcaatata ttcgtaaccg
    18481 tcgtctcgat ttttgtgctg atgcgattcg ccatgccgca gatgatgaaa aactggcagg
    18541 tattggcttt cattggggat tttccgatca gagtcatttt tcaacggtat ttaagcaacg
    18601 ctttgggatg acgccaggcg agtatcgacg taaattccgc taactaattc gcgttgcatg
    18661 tacagcaacg cgaattagtt aaaacagata tttgactatg catttttcag ggcgaaaggc
    18721 aatttgacag gaatttttga atattcacct tgtttccgga aaatagaata atcgccttct
    18781 cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc catttaccgt
    18841 ttatcccttg tacatattcc cccggtttag cccgagctac gagcttttgc ccggcgagtt
    18901 tcgcgatatc atctaccgag acattatttt gctttgccag ttgttggtaa ctcgctttac
    18961 gttcggcatt aatgtcgttt actaatttct ctgtttcagc atctgttttc agcgcaacca
    19021 gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc agagtcaatg
    19081 ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat gttttcttca
    19141 tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc tttgtctgcc
    19201 ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg ttccttaggt
    19261 gcagcgactt caatgcgagg cgtacagcca accagcataa aagatgacgt caacgcagcc
    19321 agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca tttcccggca
    19381 gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca
    19441 gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt ttaccatcaa
    19501 cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta tctaaattaa
    19561 tatttgtcga tgaacggcta atttccatat agcgcaacca gttaattgct gaacccgcag
    19621 tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag gtcattggcc
    19681 cgctatttgc cagccagcca tctttcacta tccatttttc gttgtttagc cataacggca
    19741 atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg ctaaccagtt
    19801 cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt
    19861 gtttcatcac cacgttaccg cctaacacat cgacgctaac atcagtcaac agcaatggtt
    19921 cttcttctgt ccacggatag cgcccttgca aatcagccgt aatatttttc gctgtcacca
    19981 gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc
    20041 catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc
    20101 aggcactacc gcctttcaac acaccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag
    20161 cagaaaatgc gacctgagca tacagttcgc catcgcgtaa gttcatcttc cagtcgggtg
    20221 gcaccagcgg ctggaatacg gtcagtgatt gtttaggcca ccaggcgtta ccgcgcagac
    20281 gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc
    20341 ctttaaagag gaaatacgtc ggatcgcgtc catcgacgct aaattttaac gttgatggcg
    20401 gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga
    20461 aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct
    20521 tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg
    20581 taatcgtgct gtcatgccac tcaccctttc ctgcgacatc ccaggtggca ttcatcggta
    20641 taaaactgcc ttttccccag tagcgccact gccagcggcc agcgtcaggg agaaaatcat
    20701 tcgccagtcc atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa
    20761 tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc
    20821 ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat aacgcacctg
    20881 gctcaaaggc caacgttggg tcagtcagac ttccgcttag ctgcgcaggt aaacgggcat
    20941 ataaaatgag gtccgcctgt ttcgcttcac cggtcagttg cagaggcagc tggctgttat
    21001 ccatgcttaa ttttcctggg ccaaaattaa gcaccgcgtt gcccttaccc gcttgcccct
    21061 gggtcagcac actcagtcgt ccgctgacca gagcgttctc aagccctgcc tgccagttgt
    21121 cgactttaac acccagtcgg ccactcaaag gaaaacctgc atacggccag ctccagcgac
    21181 catcgcttac ggtcaattgt tgacgagtaa tttgccacgg cagatcgagc aacggatcgc
    21241 cgttatcccg tgccaccaca atcaattgcc cgctattttc ctgccagtcc agctcggcat
    21301 ccaccagtga aggctcctgc gacaagttta acgtcgcagt agcatgacca cttacaggaa
    21361 gtccatccgg cactagcggc atagtaaatt cccccaccag ttttaccggc ggctgatttt
    21421 caaacgcgac gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa
    21481 atttaacttt ttcgccctga taacgcagtt gctggatatc ggaggttaat gcgagagaga
    21541 gttttccctg ccactcctgc cagggagaaa aaatcagttt atcgatattg atccaggtgt
    21601 taggcaacat ggcctgccac tgcgcgaggg tttttggagc ggcaagcgat tgctccgtct
    21661 gcggcaattt cgccagacaa gcagaatcaa attctaccgt gccgacgttc aataaccagc
    21721 ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat
    21781 aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa
    21841 tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacaag ccgagcgtca
    21901 tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt
    21961 tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta
    22021 ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttga
//
LOCUS       DARNVM010000069        21717 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.069, whole genome shotgun sequence.
ACCESSION   DARNVM010000069 DARNVM010000000
VERSION     DARNVM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.069"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1336
                     /gene="paoC"
                     /locus_tag="SI621_002419"
     CDS             <1..1336
                     /gene="paoC"
                     /locus_tag="SI621_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HEI4065130.1"
                     /translation="LRIGTDQSGKITAISHESWSGNLPGGTPETAVQQSEFLYAGANR
                     HTGLRLAMLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPA
                     DPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSG
                     AWVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGS
                     GGQWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGG
                     RLTAEESIEFGILSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTA
                     RSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDP
                     ISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1346..2299
                     /gene="paoD"
                     /locus_tag="SI621_002420"
     CDS             1346..2299
                     /gene="paoD"
                     /locus_tag="SI621_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HEI4065131.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRAILRFAVEALMSSKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGHYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETEQ
                     IRAPVGIFPKARDAHTLALSVLAEVAAVRLRQEDSCLPPSS"
     gene            complement(2478..3605)
                     /locus_tag="SI621_002421"
     CDS             complement(2478..3605)
                     /locus_tag="SI621_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065132.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLQLGMTVLMAVSGLIIALASSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSSILLIIGVAGFIGTLVVSAFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLAIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3747..4805)
                     /locus_tag="SI621_002422"
     CDS             complement(3747..4805)
                     /locus_tag="SI621_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4065133.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5070..5954
                     /locus_tag="SI621_002423"
     CDS             5070..5954
                     /locus_tag="SI621_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4065134.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            complement(6388..7308)
                     /locus_tag="SI621_002424"
     CDS             complement(6388..7308)
                     /locus_tag="SI621_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEI4065135.1"
                     /translation="MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAVLKDKNPAKYVAAVNKLERVLA"
     gene            complement(7295..8077)
                     /locus_tag="SI621_002425"
     CDS             complement(7295..8077)
                     /locus_tag="SI621_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEI4065136.1"
                     /translation="MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLTSLALQGHAHYTEQGKPQVWLAL
                     PAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
                     CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYI
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRAFKPEGVEDGSA"
     gene            8393..11602
                     /locus_tag="SI621_002426"
     CDS             8393..11602
                     /locus_tag="SI621_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEI4065137.1"
                     /translation="MTYQAIFTGWDDLTIEDLLVAYRKAKADSFFENTFPVAIKFAEY
                     EQELLENLQKLLDLLQSEDGFSSNKKLIGKFRLLPKKLTTKKKHESQNGHVHFSNPKR
                     AADHLFNNFDLIPEFRIIGDFPVDSHIISALWINMVGHKFDASLDNCCYGARLKRIRN
                     DELFSNEQDNPFHISAVGSFSPYFQPYQKWRGDGLKAIRDELEKDRDIIAASLDLKSY
                     YHFIDPLAITSDDLYNTLNIKLTEDEKAFTAQLAVFLKHWSDGAAAFGKKIAYKTPVI
                     NGGLVIGLTASRIISNILLHHWDKLVIEKLSPIHYGRYVDDMFLVIRDTGTITNNHEF
                     MLLLQDRLGNDCVYLKNEQKQIWQIQQGEHFQGKTTIQLQSDKQKLFVLQGRAGIDLL
                     DSIEKEIYELSSEHRLMPSPDQLEHSTAAKVLSAAGSVGENADTLRRADGLTIRRLGW
                     SLQLRYVETLARDLPPSEWKEQREEFYQFAYNHILRADNLFAHFSYLPRLLGFAISMN
                     EWQHAEKIVLKAYESINLLASVITSGKEVNINGCKTRAVNDLWRCIKGTLSWLFVDAA
                     TRYYSPDRLFLDKRSKKEECLADTFFNHISQSLTNLKDLLDLRFDSADFYLKAPLVAR
                     ADLAKEPYKQIVKSQSAEKLVNQRDSKKEVKILKLMSDSSLIDIDVIKLFLKSTKNTR
                     LEKVAKGNRKNESYLPYIFPTRPLTPAEISELAPECVGLPSTSDKKPDERPSTIWAKY
                     TQALRGVWIKPTLLASEQDSDEATKKARPKKFIHIGTDRKHKVVVALTSIKTEEDDWA
                     KMACNKSNLSRSRYQRISELVNATLKLSPKPDYVLFPELSIPLRWVNSIADRLSSAGI
                     SLIAGTEYRHLDDNQLKSEAVLVLSDNRLGYPASVKIWQPKLEPAVGEDEALFSIYGK
                     SWDSTLNVKQRKPVYIHHGVNFGVMICSELQNSKARIRFQGALDALMVLSWNKDLDTF
                     ASLIESAALDIHAYTILVNNRKYGDSRVRSPAKEPFMRDIARVKGGDNDFVVAATLDI
                     DSLRAFQSRAKRWPKGGDKFKPLPEGFQLAKNRKKLPPK"
     gene            11763..11903
                     /locus_tag="SI621_002427"
     CDS             11763..11903
                     /locus_tag="SI621_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HEI4065138.1"
                     /translation="MTRQRLFLCCSHGYISMVGRAGASQDAPVSDKAGKANPARFHHP
                     RD"
     gene            11949..12419
                     /locus_tag="SI621_002428"
     CDS             11949..12419
                     /locus_tag="SI621_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065139.1"
                     /translation="MATILTTDSDLLDLLTVPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLTESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKMEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFISEVAA"
     gene            12416..12518
                     /locus_tag="SI621_002429"
                     /pseudo
     CDS             12416..12518
                     /locus_tag="SI621_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773560.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            12610..12768
                     /locus_tag="SI621_002430"
                     /pseudo
     CDS             12610..12768
                     /locus_tag="SI621_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(12783..12858)
                     /locus_tag="SI621_002431"
     tRNA            complement(12783..12858)
                     /locus_tag="SI621_002431"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12823..12825),aa:Thr,seq:cgt)
     gene            complement(12973..14226)
                     /gene="proA"
                     /locus_tag="SI621_002432"
     CDS             complement(12973..14226)
                     /gene="proA"
                     /locus_tag="SI621_002432"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEI4065140.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESSVALHADVAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(14238..15341)
                     /gene="proB"
                     /locus_tag="SI621_002433"
     CDS             complement(14238..15341)
                     /gene="proB"
                     /locus_tag="SI621_002433"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEI4065141.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            15629..16684
                     /gene="phoE"
                     /locus_tag="SI621_002434"
     CDS             15629..16684
                     /gene="phoE"
                     /locus_tag="SI621_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HEI4065142.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(16723..17124)
                     /gene="crl"
                     /locus_tag="SI621_002435"
     CDS             complement(16723..17124)
                     /gene="crl"
                     /locus_tag="SI621_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HEI4065143.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(17182..18426)
                     /gene="frsA"
                     /locus_tag="SI621_002436"
     CDS             complement(17182..18426)
                     /gene="frsA"
                     /locus_tag="SI621_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HEI4065144.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(18518..18976)
                     /gene="gpt"
                     /locus_tag="SI621_002437"
     CDS             complement(18518..18976)
                     /gene="gpt"
                     /locus_tag="SI621_002437"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEI4065145.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            19237..20694
                     /gene="pepD"
                     /locus_tag="SI621_002438"
     CDS             19237..20694
                     /gene="pepD"
                     /locus_tag="SI621_002438"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HEI4065146.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(20751..21103)
                     /gene="prfH"
                     /locus_tag="SI621_002439"
                     /pseudo
     CDS             complement(20751..21103)
                     /gene="prfH"
                     /locus_tag="SI621_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(21099..21305)
                     /locus_tag="SI621_002440"
                     /pseudo
     CDS             complement(21099..21305)
                     /locus_tag="SI621_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(21551..>21717)
                     /locus_tag="SI621_002441"
     CDS             complement(21551..>21717)
                     /locus_tag="SI621_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEI4065147.1"
                     /translation="LKPLIKSESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFT
                     NFYMRYKPQH"
BASE COUNT         5522 a         5589 c         5437 g         5169 t
ORIGIN      
        1 cttgcgtatc ggtactgacc agagcgggaa aatcaccgct atctcacatg aaagctggtc
       61 cggaaacctg cccggcggca cgccggaaac ggcggtacag caaagcgaat ttctttacgc
      121 aggggcgaat cgtcataccg gcctgcggct cgccatgctt gatttgccgg aagggaacgc
      181 catgcgtgcg cccggcgaag cccccggtct gatggcgctc gaaatcgcga tcgatgaact
      241 ggcggaaaaa gcgggcatcg atcccgtcga gtttcgcatc ctgaatgaca ctcaggttga
      301 ccccgccgat ccgacgcgcc gcttctctcg ccgtcagctt atcgagtgct tgcgcaccgg
      361 agcggataaa tttggctgga agcagcgcaa cgccacaccc ggacaggtac gcgacgggga
      421 gtggctagtc ggccacggtg ttgcggcggg ctttcgcaat aatctgctgg aaaaatcggg
      481 tgcgtgggtt cacctggagc aaaacggcac cgttaccgtg gaaacggaca tgaccgatat
      541 tggcactggc agctacacca ttctggccca gacggcagcg gaaatgcttg gcgtaccgct
      601 ggagcaggtt gcggttcacc tcggcgattc cagtttcccg gtttctgcgg ggtctggtgg
      661 acaatggggc gcgaatacct ccacctccgg cgtttacgcc gcctgtgtga agcttcgcga
      721 aatgattgcc tcggcagtcg ggtttgatcc tgagcagtcg cagtttgccg acggcaagat
      781 taccaacggt acccgaagcg ccacgctaca tgaggccacc gcaggcggca gactgacagc
      841 ggaagagagc attgaattcg gaatactgag caaggagtac cagcaatcga cctttgccgg
      901 gcattttgtg gaggtcggcg tgcatagcgc gacgggagaa gttcgggtcc ggcgtatgct
      961 cgccgtgtgc gcagcggggc gcattctgaa tccgaaaact gcacgcagcc aggtcattgg
     1021 cgcgatgact atgggcatgg gcgcggcact gatggaggag ctggcggtag atgaccgttt
     1081 gggctacttc gttaatcacg atatggcggg gtatgaagtg ccggttcatg cggatatccc
     1141 aaaacaggag gtgattttcc tggatgatac cgaccccatc tcctccccga tgaaggccaa
     1201 aggtgtcggt gagctgggcc tgtgcggcgt gagcgcggct atcgccaacg cggtgtataa
     1261 cgccaccggt attcgggtac gggattatcc catcactctg gataagctgc tcgataagct
     1321 gccggatgtg gtttaaggag gaacaatgtc atactcgctt tttgacaaag acgagcactg
     1381 gcataagcca gagcaggcgt ttctcaccga tgaccaccgg gccattctgc gcttcgccgt
     1441 agaggcgcta atgtccagta aaggagcggt gctggtgacg ctggtggaaa tacgcggcgg
     1501 cgcggcgcgc ccgctcgggg cgcagatggt ggtgcgcgaa gatggtcatt actgcggttt
     1561 tgtctctggc ggctgcgtgg aggccgctgc cgcttttgaa gcgctggaga tgatgggctc
     1621 aggccgcgat cgcgaaattc gctatggtga aggttcgccg tggtttgaca tcgttctgcc
     1681 ctgcggcggt gggatcacgc tgacgctcca taaactacgc tcggcacaac ctctgctcgc
     1741 cgtgctgaac cgcctggaac agagaaaacc ggcgggtctg cgctacgatc cgcaagcaca
     1801 atcgttggtg tgcctgccca cgcaaacccg gacgggctgg aatctcaatg gctttgaggt
     1861 ggggttcaga ccatgcgtca ggctgatgat ttacggacgt tctcttgagg cgcaggcaac
     1921 cgcgagtctt gcagcagcca caggctatga cagccatatc ttcgatcttt ttccggcctc
     1981 agccagcgct cagatcgata ccgatacggc ggtcattttg ctgtgccatg atctcaaccg
     2041 ggaactgcca gtgttgcagg ccgcgcgaga agcaaaaccc ttttatctcg gcgcattggg
     2101 cagctatcga acccacactt tacgtctgca aaagctccac gagctgggat ggtccaggga
     2161 ggagacagag caaatccggg cacctgtcgg gatattcccc aaagcccggg atgcgcatac
     2221 tctggccctt tccgtactgg cagaagtcgc cgctgtacgt ctccgtcagg aggattcatg
     2281 cctgccccca tcgtcctgat cctcgcggcg gggcgtgggg agcgcttcct cacctccggg
     2341 ggaaataccc ataaattaag agaaaaatct gcagtagccg tttaagaaaa tcacctaaat
     2401 cgtttaaaag agcgcccaac ccatagtggg cgcttatcgc cttgtgaaac ttattcagca
     2461 acgcgcgcct cgccggacta aagcgcaccg gccttctggc gtgcggtaac aaaggtcagt
     2521 acgccagcgc aaaggagcag cacgctactc atagcaaacg tgctctgcca gccaaggcgg
     2581 tcaaacacaa tgcctcctgc cgttgatcca agggcgatgg cgagctgaat caccgcgacc
     2641 atgagcccgc ctccagcttc ggcgttgtca ggcaacgtac gcgcaatcca tgtccaccat
     2701 ccggttggcg cggcggtggc cagcatgccc cagagtccta acagcagcga gaccgtccag
     2761 acgtgatgcc cggtaagcat cagaccgatg gcaatgacgg ccatcagtcc gggtatggcc
     2821 atcagcgtgg ggtagaattt tctgttgaga aatgccgaaa caaccagcgt gccaataaaa
     2881 ccggccacgc cgataatcag caaaatcgac gacaagccta aggaattaac ccgtgtcacc
     2941 gactccagaa acgggcgcac ataggtgaat agcgcaaact gccccatgaa aaacaggcca
     3001 caagctatca tgccgaccac aaccatgcgg cggctaaata agcggaatac cgcgccacat
     3061 gaagtgtggc ttttattcgc atccatgctt ggcaggctga tgcactgcca gataaaggcg
     3121 acaaccgcga tgggcaccag gcacaagaaa gcgcctcgcc atccgaccgt agcccccaga
     3181 tagcttccca gcggtgcggc cacgaccgtc gccagcgcat taccggcgtt gaaaattgcc
     3241 agcgcacgcg tgacctgatg ctgcggcacc agacgaatcg ccgttgccgc tgacatcgac
     3301 cagaatccgc caatagccac accaatcatc gcacgaccga ccatatacat cagataactg
     3361 gaagccagcg caataataag ccccgatacg gccatcagaa ccgtcatccc cagctgcagg
     3421 aacttacgat tcatctttcc agctagcgtt gaaagcgtca ggctggtcag aaccgccagc
     3481 gcgccggaga tagcaatccc ctgccctgcc agcccctccg tcacgcctaa atcgcgggta
     3541 atcggcgtga gcaggctgac gggcataaat tcagaagcaa tcagcacaaa cacgcacagg
     3601 gtcatggcaa aaataccgcc ccagtatgcg cgctgatgat gaggtgatag tgtttggctt
     3661 agtgtcgaca tagtgaaatt tcacaaagat caggataaaa gccaccagcc tatggccggt
     3721 ggctttgcat caattcagga gaggttttat ttcagtttgg tctggaaaaa ttgttcgaac
     3781 ttcgcgaaag gtatttttcc ggccacgtta tcgtacaggt ccacgtgatt tgcccctggt
     3841 accaccacca actctttgtc tttactgcca accgccttgt aagcatcttc agcaaaatag
     3901 cgtgaatgtg ctttctcacc ggtcacaatc agcgtcggaa tggtgatttc actggcgtag
     3961 ctcaacagcg gcatattcat aaatgacagc ggcatcgttg ccgtccacgc cccggtagag
     4021 ttaacggaac gttcgtggaa gccgcgcggc atgcgatagt agtcaaagaa ctctttcagt
     4081 accggatgag gatttgccgg cagcgtttct ggcagaatgc gctcgcccgc actgaccttg
     4141 ccgttgctgt cgacattaat atcatgacca ccgtgagcga acgttccgct ctgcgcatcc
     4201 ttccagcgct gttcattcag atattgcaga acagcacgac ggtcggcggt agaataacgg
     4261 tctttgccat cccccacacc atggcccatc gcccggctca tgtcatacat cacgctggta
     4321 gctaccgctt taacgcgggt atccatcgcc gcgtcgttca gcgccatgcc gccccagcca
     4381 caaataccga gcagcccgat acggttgcga tctacctctt tttgcagccc taagaaatcc
     4441 actgccgcgc tgaaatcttc agtgttgata tccggtgagg cgacgtttcg cggatagccg
     4501 ccgctttccc cggtgtaaga aggatcgaac gccagggtaa caaaccctcg ttccgccagc
     4561 gtctgcgcat acaggccgct ggattgctct ttcaccgcgc caaatggccc actgaccgca
     4621 atcgccgcca gcttgcgatc gccacggtct ttaggcaggt aaagatcgcc cactaaggtg
     4681 atgccatatc ggttcgggaa cgagacttta cggtgatcga ctttctggct ctcggcgaac
     4741 gttttatccc atttatctgt catggacaca ggggcattag gattggttga atcagcataa
     4801 ctcattgtcg tgactccact taatgatgcg aaaagcagca tggtcggcat cgcggttttt
     4861 agcgtgcggg tgaaggcttt cataaaaatc cccataaaaa acaacagaga gatacaggtt
     4921 tccgacggac aacgctgcgg tgctttactt tgatggggtc attatagtga gcagaattag
     4981 tgctgattag ggggcgatta atgctaagat taataccata ttgttataaa ttaatgtgga
     5041 gattgccggc atgaaggggg gatgtttcaa tggcgaaaag ggagaactac aacgagctgt
     5101 acctgtttat gcaggtggtg cgggaaggca gctttaccgc agcggctcag cggctgggtc
     5161 ttgctcagtc aggagtcagc cgttctgttc gcgagcttga agaaaggctg ggtgtccagc
     5221 tgctagtgcg caccacgcgt aaactgtcgc tgacacaagc aggcgagcag ctctaccaga
     5281 agacggcatc tggatttgaa atgttagatc ttgggcttgc cacgctggcg cattatcgag
     5341 agacgccctc cggcacggtg cgtatcaatg ccagccagca cgcgattgat aaatgcctgc
     5401 tgccaaagct ggcggtattt aaacagagat atcctgatat ccggcttgaa ctgataaatg
     5461 agagccggtt cgtcgatatc atcgaggaaa gattcgatgc aggtgtccgc ctggggcctg
     5521 aagtcggcca gggtatggtc gcggtacgca tcacgcccga tatggagatg gcaattgtgg
     5581 ggactccgga acattttcgt cgctatggtt ttccgcaaac cccggcggat ttaaaggcgc
     5641 atccctgtat cgcctatcag tttgccgacg gcagcgtata ccagtgggaa ctcaatcagg
     5701 atgataaaaa aatcactcat cgccctgaag ggcaatgggc cctatccgac agctatatgg
     5761 aagcagaagc cgcccggctg ggcctcggat tggcctatgt tcctgttgaa ctggtcgcgg
     5821 atgatttaga acacggtaag cttatcaggg tgttgcagcg ttacagcctg cgaatggagg
     5881 gattgtttct ctattaccct catcgcaacg tctcccccgc cctgcggatg gtcattgata
     5941 cattgaaaat ctgaacgtta acaacgaccg actcttcact gtgcaaaatt gtgtcaggta
     6001 gtccctctgc gttcaattcc agggtctgct ttgagcgaga aagaggggac tatccagcaa
     6061 tggagatcac ccaaaagaaa aaaccacctg gctccctcga caaccttcat ccgggcattt
     6121 agcctcaatc aacattttta tatctgtcag gttacttcat tatgaaaact gatcatcatc
     6181 gttgtttttg ttgcaatagt agaatacgcg tatttattcc tccatctcta ttaataacgg
     6241 caataaactg ttcacttcac tgatatttaa aatatgtatc cttttcaagc tgttcagcgg
     6301 cgtaagaaaa agtattaatt atgaattcga tgatttaacc atatctgtct gattggtaat
     6361 catccgtgat taatgctggt ctattgatta cgccagaacc ctctctagct tattcaccgc
     6421 cgccacatac ttcgccggat ttttatcttt aagcaccgcc agattgcgtt gcttccagcg
     6481 aatagccgga agatgctgaa tttcaggata gcgataccgc gaccagtcag ggctgttttg
     6541 cttaaaactg agcaagaact cgcgatccag atccgtaaga tgtgacttca acgcctgcgg
     6601 cagcaaagtc gttactgaca gaagcgattc cagcgacgtt tcctgttgcg tcatccctga
     6661 aaattcctgc gcgtacaacg tggcgatccg ctctgtatcc cagtttggag ccagcaactc
     6721 ggcgatgggg cggggatggc ctgccagata acaaagaaaa ccgtcaaaga tctcccgcat
     6781 cagtcccggt ttctccagca tattcagcac atcaaacaga tcgcgcgggt gctgacgatc
     6841 aagcgcagcg cagattttac cgccataaag atccgccagc gagacgacac tcatggtggt
     6901 gaagccatac tccatttcca cacgttcgca cacaggcatt tctgccggag gcaacaacag
     6961 cccgcgccag accgggctaa cctcgatctt aatctttgca tcagcggtat tcacaatgat
     7021 gcgcttttca tccgctttat tttcctgccg aatcgcggtg atccccggtc tgccgttcag
     7081 cgatgcagta atcctcttca aagcgtcgtt gatagccgcc aaatcagtat cgcgatccgc
     7141 aaagctcttg tacgccagat caatatccac cgacagacgg ggaaaatcct gcacaaacaa
     7201 attgattgcg gtgccgcctt tcaacgcgaa acaggactcc ttcgccacat acggcagagc
     7261 ggccatcaac agtttgacct gccgggcata gctatcacgc agatccatct tcgactcctt
     7321 ccggtttaaa tgcgcgtgga acagtaattt gataatccgg ctccagccag ccgccgggta
     7381 tgatttgccg ctttcccgta cctgtttcta taccgctttg atcaatatac tgaaaataag
     7441 ggtgtccatt acgccgggca aggtaaagca tcacccggtt ggttttaacc gaatggctgg
     7501 cgcgaagcag tgcctgcaat ttgcgtgggc tgagaagatt caacccctga aacagagcat
     7561 cggcatgttc gaacgagatt aacgaaggaa cgccgcaggc gatctcatac gcggccagtt
     7621 ccggaacact gcctgctaac tggcggtcgc caattttcag ttccgtagta aatggcgtta
     7681 catcagcggt aaggcctgac gtatacagcg tgacaaacgt tgcggattgt tcaaaggcag
     7741 aaaaccatcc cggcagtttg acgtaagccg gaagcgcgag ccagacctgc ggcttaccct
     7801 gttcggtata gtgcgcatga ccctgaagcg caaggctggt tagccccgcc acccgaacag
     7861 gcttttccca ctgcgattgc aggcagtaaa ccgcatccac ccaatccggt tcacgacccg
     7921 tccggcagta tacgccgtga gcgatccgcc tgagccagcc attcttcaca tagagataag
     7981 agagcttacg atctatgccg ttgcgggtaa gccatgactg gagcagcacc tggccgggta
     8041 gcgtgtgctg catcagccag tttagatagt ttggcaactg tcgcctctga atagacatta
     8101 gtacaaatat ttaaaccagt gtagattcag ggatacgcca cagcaagaaa tagtttactt
     8161 attcctcatt ttgtcgacta aaaatcgaca ttaaacaaaa aattcaaact taatcacttt
     8221 cgggaaaaat gtgacaaata tatgctcgga ctggttgcgg ggagcgtgta acatggatac
     8281 aaatcaaaat tattgccagc ctcactgatg gattactggt gtcaagagcc ccccttcggg
     8341 catgaaacgg ctggctaatt ctgtacagac tgtaatctaa ggacgataac gcatgacata
     8401 tcaggcaatt ttcactggct gggatgatct gacgattgaa gaccttctgg tcgcttaccg
     8461 gaaagcaaaa gccgatagct tctttgagaa tacatttcct gttgctatca aatttgccga
     8521 gtatgagcag gaattacttg aaaacctgca aaaactctta gatcttttgc agagcgaaga
     8581 tggattcagt agcaataaga agttgattgg caaatttcgt ttgttaccga aaaaattaac
     8641 cacaaagaaa aaacatgaat cccaaaatgg acacgtccac ttttctaatc ctaaacgagc
     8701 agccgaccat ttatttaata attttgatct gataccagag tttcgtatta ttggtgactt
     8761 cccggttgat agtcacatta tctctgcact atggattaac atggtcgggc ataaatttga
     8821 tgccagctta gataactgtt gctatggcgc gcggctaaag cgtattcgta atgatgaatt
     8881 atttagcaat gagcaggata atccattcca tatcagtgcc gtgggttctt ttagccccta
     8941 cttccagccc taccaaaaat ggcgtggtga tggcttaaaa gctatacgtg acgagttgga
     9001 aaaagatcgt gacattatcg ccgcctcact ggatttaaaa agttactatc attttattga
     9061 tccactggct ataacctctg atgatctcta taacacacta aacataaaac tgactgagga
     9121 tgaaaaagcg tttactgcac agttagcagt attcttaaag cactggtctg acggcgcagc
     9181 ggcatttgga aagaaaatag cgtacaaaac acctgttatt aatggtggtc tggtcattgg
     9241 attaacagcc agtcggatca tttcaaatat attgctacac cattgggata aattagtcat
     9301 tgaaaaacta tcaccaattc actacggtcg ttatgtcgat gatatgttcc ttgtaatacg
     9361 cgatacaggg acaattacta ataatcacga atttatgtta ttgctgcaag ataggcttgg
     9421 caatgattgc gtttatttga aaaacgagca aaaacaaata tggcaaatac agcagggcga
     9481 gcatttccag ggtaagacca ccatccagtt acaatccgat aagcaaaaac ttttcgtgct
     9541 tcaagggagg gctggaatag acctgctcga cagtatcgaa aaggagatct acgagctttc
     9601 tagtgaacac cgcttgatgc cttcaccgga tcaactggaa cactccaccg cagctaaagt
     9661 cctttccgct gccggtagtg taggtgaaaa tgccgatact ctgcgccgtg cggatggatt
     9721 aaccattcgt cgtttgggct ggtcactgca attacgctac gttgaaacac tggcacgaga
     9781 tctgcctcca agtgaatgga aagaacagcg ggaagagttt tatcagtttg cctacaacca
     9841 tattcttagg gctgataatc tatttgcaca ttttagttat ctgccaaggc tgcttggctt
     9901 tgctatcagt atgaatgaat ggcagcacgc ggaaaaaatt gtacttaaag cttacgaatc
     9961 catcaacctg ttggcatcgg tgattacttc aggtaaggaa gtgaatataa atggttgcaa
    10021 aactcgagca gtaaatgatc tttggcgctg tataaaaggc acattaagct ggctatttgt
    10081 tgatgcagcg acacgatatt acagtcctga cagattattt cttgataaac gttcaaagaa
    10141 agaagagtgc cttgcggata cattttttaa tcatatttca caaagtctga cgaatctaaa
    10201 ggatttactg gatcttcgct ttgattcagc agatttttat ttaaaagcgc cattggtagc
    10261 tcgagctgat ttagcaaagg aaccttataa acagatcgta aagagtcagt cggcagaaaa
    10321 acttgttaat cagcgtgata gtaaaaaaga agttaaaata ctgaaattaa tgagcgactc
    10381 atcgcttatt gatattgacg ttattaagct atttttgaaa tcaaccaaga atacccgact
    10441 ggaaaaagtg gctaaaggaa atcgtaagaa cgaaagttac ctaccttaca ttttccctac
    10501 acgtccttta acacccgctg aaatatcaga actggccccc gaatgtgttg gattaccctc
    10561 cacatccgac aaaaaaccag atgagagacc gtccaccatt tgggcaaaat atactcaagc
    10621 attacgcgga gtatggatca aaccgacgtt gctagcatcg gagcaggact cagatgaagc
    10681 gacaaaaaaa gctcggccta agaaattcat tcatattggc acagacagga aacataaagt
    10741 tgtcgttgcg ctaaccagca ttaaaacaga ggaggacgac tgggctaaaa tggcctgcaa
    10801 taaatctaac ttgtcccgtt caaggtacca gcggatttct gaactggtta atgcaacatt
    10861 gaaactatct cctaaacctg attatgtttt attccctgag ctttcaatcc cgttacgctg
    10921 ggttaacagt attgctgatc gtttgagttc ggcgggtatc agtctaattg cgggaacaga
    10981 ataccgccac ttagacgata atcaactgaa gagtgaggcc gtacttgtcc tttcagataa
    11041 cagactcggc tatccagcga gtgtcaaaat atggcaaccc aagctggaac ccgccgtagg
    11101 tgaagatgag gcattatttt caatttatgg taagtcttgg gattcgacac ttaatgttaa
    11161 acaacgtaag ccggtatata ttcatcacgg cgtcaatttt ggcgttatga tttgctctga
    11221 actccagaat agtaaagcga ggatccgttt tcagggcgca ctcgatgcat taatggtatt
    11281 gagctggaat aaagatctag atacgtttgc atcgttgatt gaatcagcag cgctggatat
    11341 tcatgcctat actattttag tgaataaccg aaaatacggc gatagtcgcg tacgttcccc
    11401 ggcaaaagaa ccctttatgc gtgatattgc tcgtgtgaag ggcggtgata atgactttgt
    11461 ggtcgctgca acgctggata tcgactcgtt aagggcattt cagagcaggg caaaacgctg
    11521 gcctaaaggc ggcgataaat tcaaaccgtt acctgaagga ttccagttgg caaagaaccg
    11581 caaaaagcta ccgccaaaat aagaaactga ttttcgctat taataatcag ggtatttttg
    11641 cgtgagatgt tggtaaacat gatgtagccc ttgccactca tgaccaatcg cagtatcttt
    11701 ctcccgcgcc tgcaaaatca ggcgtcggga ttagcctcct gaagaaatct tatcggcgac
    11761 acatgacgcg ccagcgtctt tttttgtgtt gttcgcacgg ttacatctca atggtgggcc
    11821 gggcgggggc gtcgcaagac gcgccagtgt ccgataaggc tggtaaggct aaccctgccc
    11881 ggttccacca cccgcgagat tagcctctcc ggtggtggct tgaacaccca cttatcggag
    11941 gttgccctat ggctacaatc ctcaccactg acagcgattt actcgatcta ttgaccgtcc
    12001 ccttcaatgc caccacggat ttcactgtcc ttgctgacta ctgcgcccgc tttgccgaaa
    12061 ccctgattga aaccgacgat ccagcgctac gactggcgct ctgcgggcgg cttcacgcgg
    12121 ctcttgcgct actgcaaccc acactacacg accccatccc accgcatctt accgaaagcc
    12181 tgaccgtgga tacccttccc gccacaccag agcactttat ttccgaacct gattccttgt
    12241 gcgaatactg cctcgcacta ctccagcttc tgctggaacg tccggcggaa ccaaaaatgg
    12301 agcaaaccct gaccgggctg ctgtgtgagc tggtctggta tttcacggaa ggactcaaag
    12361 ccccacgctg gctgcgtacc gccgacggcg taaaatttat cagcgaggtg gccgcatgat
    12421 gcaacaggac tggcattccg ccgacattat tgcggtgctg aaaaagcggg gaacgcctct
    12481 ggcggcacag agaaaaactc attgcggaag cgctgtaaat atcgccagaa atcatctggc
    12541 cttcaaggta ctttgatagc aaaggcaagc aggctggttc agtccagtgt ttctcttctt
    12601 gcattttcag aaatgggagg ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc
    12661 gcgcctaatc accttaccga acacgcacgg caaatagact cagtcctgaa cccatcggtc
    12721 ccaaatttgt cccagtcaaa aaataaggaa ggtactaatg atgtgtaact tattgatttt
    12781 aatggtgccg ataagaggag tcgaacctcc gaccttcgca ttacgaatgc gctgctctac
    12841 caactgagct atatcggccc tgaaaggaca tgttcacgaa cgtgaatcac ggtggacaag
    12901 gttaaaacta accgggcgat gcgtcaatgg ccttgtgaat caaatggcta cttttgcatc
    12961 acccggtttt atttacgcac gaatggtgta atcaccaatg ccgatccact tgtaagtggt
    13021 cagtgcttcc agccccattg ggccacgcgc gtggagtttt tgtgtgctta ccgccacttc
    13081 cgcacccaga ccaaactggc cgccatcggt aaaacgcgta gaggcgttaa cgtaaacagc
    13141 ggacgaatcc acttcgttaa caaaacgctg ggcgttgcgc atatcgcggg tcaggatcgc
    13201 atcggagtgt tgtgtgccgt gttcacgaat atgggcgatg gcatcgtcaa gatcgctgac
    13261 gattttaacg ttcaaatcta atgacagaaa ctcatcgtca tactcttcgg ctttaacagc
    13321 caccaccttc gcggggcctg cctgcaactg cgccagcgca gctacatctg cgtgtaatgc
    13381 cacgctgctt tccgccattt gcttgcttaa tgcgggcagg aagctatcgg cgatgttttt
    13441 atttaccagc aacgtttcta ccgtattaca tgtgctcgga cgctgagttt ttgcgttgac
    13501 gattactttc agggcttcag cgatctctgc actttcatca acgtaaatat ggcatacgcc
    13561 tataccgcct gtgatcaccg ggattgtcga ctgttcgcgg cacagcttat gcaggccagc
    13621 gcctccacgc gggatcagca tgtcgatgta tttatccata cgcagcattt cactgaccag
    13681 cgcacggtca ggattatcaa tcgcctgcac ggcacccgcc ggtaagccgc aggatttcag
    13741 ggcgtcctga atcaccgcta ccgttgcagc gttagtgcga caggtttctt tgccaccacg
    13801 caggatcacc gcgttaccgg ttttcaggca cagcgaagcg acatcaaccg tcacgttcgg
    13861 gcgcgcttca taaatcacgc caataacccc cagcggtacg cgacgacgct caagacgcag
    13921 gccgctgtcc agtacgccgc catcgattac ctgccccacc ggatcggcga gattacacac
    13981 ctgacgcaca tcgtcggcga tgcctttcag ccgtgcgggc gtcagtgcca gacggtcaag
    14041 catcgcttcg ctaaggccat tggcacgcgc gtcagcaaca tcctgggcgt tagcgttgag
    14101 gatgatttcg ctttgtgctt ccagttcatc ggcgattttt tccagcacgc gatttttttc
    14161 gcggctggag agttgcgcta atttatacga ggcttgcttc gcggcaatgc ccatttgttc
    14221 cagcatcagc ctgctcctta acgggtaatc atgtcatcac ggtgaacggc aaccgggcca
    14281 tattcatatc ccagtattgc atcaatttct tgcgagtggt gtccggcaat gcggcgtaat
    14341 gcatcgctgt tgtaacgact gacgccgtga gcgatatcgc gaccttcgag gttgcaaatg
    14401 cggatgactt caccacgcga gaaattgcca gtcacgcttt taatgccttt cggcaacagg
    14461 gaactgccgc gttcaagaat ggcggcagtt gccccttcat ctaccgtgat ttcacccgcc
    14521 ggcggcgcac cgaaaatcca gcgtttacgg ttttcaagcg gagtcgcctg ggcatggaac
    14581 agcgtaccga cggaaatgcc ttccatcaca tcaccaataa cgcccggctt gctgcccgcg
    14641 gcaataatgg tgtcgatacc cgcacggcaa gccacgtccg cggcctgcaa tttggtactc
    14701 atgccgccag ttccgaggcc tgaaacgctg tcaccggcaa tcgcgcgcag tgcgtcatca
    14761 atgccgtaaa catctttaat cagttctgcc tgcggattgc tgcgcgggtc agcggtatac
    14821 aaaccttttt gatcggtcag cagcaacagt ttatcggcac ccgccagaat cgccgccagc
    14881 gcagaaaggt tatcgttatc gccgacctta atctctgccg tagcgacagc atcgttctca
    14941 ttgattaccg gaacgatatt gttatcgagc aacgcacgca gggtgtcgcg ggcgttcagg
    15001 aagcgttcac ggtcttccat atcagcacga gtcagcagca tttgcccgac gtgaatgcca
    15061 taaatcgaaa acagctgttc ccacagttga atcagtcgac tctgccctac cgccgccagc
    15121 agttgtttcg aggcgatagt cgctggcagt tccgggtaac ccaggtgctc acgtccggcg
    15181 gcgatcgcgc ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa ctgcgcgcac
    15241 tggcgaacaa gttcaacgat atgggcacgg ttcaggcggc gcgatccgcc tgttagcaca
    15301 ctggtgccga gttttaccac cagcgtctgg ctgtcactca tgattctctg ccattcaatt
    15361 ttaggaaaaa tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg ccaaccatct
    15421 gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag cgtagcggca gttgtttacg
    15481 cttttattac agatttaata aattaccaca ttttaagaat attattaatc tgtaatatat
    15541 ctttaacaat ctcaggttaa aaactttcct gttttcaacg ggactctccc gctgaatatt
    15601 cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc actctggcat tagtggtgat
    15661 gggcattgtg gcatctgcat ccgtacaggc cgcagaaata tataacaaag acggtaataa
    15721 actggatgtc tatggcaaag ttaaagccat gcattatatg agtgataacg acagtaaaga
    15781 tggcgaccag agttatatcc gttttggttt taaaggcgaa acacaaatta acgatcaact
    15841 gacgggttat ggtcgttggg aagcagagtt tgccggtaat aaagcggaga gtgatactgc
    15901 acagcaaaaa acgcgtctcg cttttgccgg gttgaaatat aaagatttgg gttctttcga
    15961 ttatggtcgt aacctgggcg cgctgtatga cgtggaagcc tggaccgata tgttcccgga
    16021 atttggtggc gactcctcgg cgcagaccga caactttatg accaaacgcg ccagcggtct
    16081 ggcgacgtat cggaacaccg acttcttcgg cgttatcgat ggcctgaact taaccctgca
    16141 atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg gcttcggtac
    16201 gtcattgaca tatgactttg gtggcagcga tttcgccatc agtggggcct ataccaactc
    16261 cgatcgcact aacgagcaga acctgcaaag ccgtggcaca ggcaagcgtg cagaagcatg
    16321 ggcaacaggt ctgaaatacg atgccaataa tatttatctg gcaactttct attctgaaac
    16381 acgcaaaatg acgccaataa ctggcggctt tgccaataag acacagaact ttgaagcggt
    16441 cgcccaatac cagtttgact ttggtctgcg tccgtcgctg ggttatgtct tatcgaaagg
    16501 gaaagatatt gaaggcatcg gtgatgaaga tctggtcaat tatatcgacg tcggtgctac
    16561 atattatttc aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc aactggatag
    16621 cgataataag ctgaacatca ataatgatga tattgtcgcg gttggcatga cctatcagtt
    16681 ttaatggata ttgccggatg tgatgcatcc ggcacatttc actcacgccg ttaacttcac
    16741 cggttcatca cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg ccagcagctc
    16801 acgcagcttc tcgtgaaatt cacgcagggt gtgctccagt cgttcaacca cttcggcatc
    16861 ttttatctga acactcgtcc agtcgcctgc tttatcgaac agaccaaact ggtaactgta
    16921 ggtaaaacgg gattcctgcg cttccagttc catccaccag ccccagaact cacgcacttc
    16981 cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc gattatcttc
    17041 gcacttacct tcacgaatat acgggcctag tgcggtaaat tttttgatca atctgctctt
    17101 cgggtgtcca ctcggtaacg tcattgcaat ctcctgttgt gatgcaactg ttttaccaaa
    17161 ttggcaaaat ttagcaaatt tttaacacaa tcgtttttca atccagtctg ttatttcctg
    17221 aagacctttg tcaaaattcc gatacaccgg gttaaatggg atctctaaca atttaccgtc
    17281 agcagatgat gaggtgatta agcgtgagtc ctcttccggg ctgaacggat cgttcttcca
    17341 gtagcctgat aacattggcg ttgggcagcg acgtcccagc aatccttgca cttttaatga
    17401 atagcgattc agctccacgc gcaacgcttc gtcggaagca tcatgcatcc ccaaacggct
    17461 cgccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat cactcaacag
    17521 ggtatgaact accggaccaa gacaggcaac cgctttcaga cgcggcgatt caaggtatgc
    17581 cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc
    17641 tacccacggt acgttaggca gcgcctttaa gacgtgctga tgcaacaggc tggagtcctg
    17701 ggtgagcttc cattttgaag aaaagcccac cgacggcata tcaatagtca gcatcgcaat
    17761 gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca tcgcatccag
    17821 accaccacac attaatactg tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc
    17881 ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc cgcatggtgc ccggcagacg
    17941 ctgagcggcc tcttcatagg cgcggtttga caaagcctgc gcttgctcgg ccaggtcatc
    18001 tcctttcaga tgaggatagg cggcaatgtt gtacaacgta gccgcatgca gccagtgacg
    18061 accactgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca tcgcctgggt
    18121 ggcccactca taaatccagt tgccaccacg gtagccaatc accgtatcgt ataaatcatc
    18181 gtcggtacgt tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcacgtgg
    18241 gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt
    18301 tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag
    18361 cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg
    18421 tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa
    18481 aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga
    18541 cgaatacgac gcccatatcc cacggttgtt cgatccaggt atcttgcggg atatcaacaa
    18601 catagtcatc aaccagcgga cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt
    18661 ttggatacat ttcacgaatc gcaaccgcag tgccaccggt atccaccagg tcatcaataa
    18721 cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt
    18781 cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca
    18841 gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc cattgttcag
    18901 aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga
    18961 tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg
    19021 aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac
    19081 atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca
    19141 aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct gtgacctgca
    19201 atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc tcaattatct
    19261 ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc gtcctatcat
    19321 gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa
    19381 cgcgatcagg taggtaatat cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt
    19441 aaaccggtcg tcttgcaggc ccacctcgat atggtgccac agaaaaataa cgacaccgtg
    19501 catgacttca ctaaagatcc tatccagcct tatattgatg gcgaatgggt taaagcgcgc
    19561 ggcaccacgc tgggtgcgga taacggcatt ggtatggcgt ctgcgctggc ggttctggct
    19621 gacgaaaacg tggttcacgg cccgctggaa gtgctgctga ccatgaccga agaagccggt
    19681 atggatggtg cttttggctt acagagcaac tggttgcagg ctgatattct gattaacacc
    19741 gactccgaag aggaaggtga aatctacatg ggttgcgcgg ggggtatcga cttcacctcc
    19801 aacctgcatt tagatcgtga agcagttcca gctggttttg aaaccttcaa gttaacctta
    19861 aaaggtctaa aaggcggtca ctccggcggt gaaatccacg ttggcctggg taatgccaac
    19921 aaactgctgg tgcgcttcct ggcgggtcat gcagaagagc tggacctgcg ccttatcgat
    19981 ttcaacggtg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac cattgctgtc
    20041 gcagctgata aagtcgacgc cctgaaatct ctggtgaata cctatcagga aatcctgaaa
    20101 aacgagctgg cagagaaaga gaaaaatctg gcattgctgc tggactctgt tgcgaacgat
    20161 aaagctgccc tgattgcgaa atctcgcgat acctttattc gtctgctgaa cgccaccccg
    20221 aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc
    20281 ggtgtggtga ccatgactga caataacgta gaaattcact gcctgatccg ttctctgatc
    20341 gacagcggta aagactacgt ggtgagcatg ctggattcgc tgggtaaact ggctggcgcg
    20401 aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg acgctaattc tccggtgatg
    20461 catctggtac gtgaaaccta tcagcgactg ttcaacaaga cgccgaacat ccagattatt
    20521 cacgcgggcc tggaatgtgg tctgtttaaa aaaccgtatc cggaaatgga catggtttct
    20581 atcgggccaa caatcaccgg tccacactct ccggatgagc aagttcacat cgaaagcgta
    20641 ggtcattact ggacactgct gactgaactg ctgaaagaaa ttccggcgaa gtaattattt
    20701 gatttgctgc cggatggcgt ttaatcgcct tccggcagtt tcatccttca ttatccttcg
    20761 ataaaagcca tccctgtaaa tgtccgtctc gggttgccac gttcaatctg gtgatggaac
    20821 attcgccgct gcgatttcag caccgcgcta ttttcctgtt gctgttgttc cagcttccag
    20881 gcaatcagca atcgcgccag ccgcttgtta gcatgcccag aaacaagttt ttgcgcccat
    20941 gatgaggccg atacggactc ggacaaatcc actgaatagt gccgcaccaa ctttcgctta
    21001 atgcccatgc gttatcgcca tccagagaaa tcagcgccga acgcagcgtg tcagagcagc
    21061 ggcccgtttc tgtttccagc accgttaccg cgacgtcttg ccgggtaaaa acgaccaaca
    21121 gagaataacg ctgcgccaat cgggtagccg cgtccggggt gtaaatctgg catccctact
    21181 acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg
    21241 atccacaggt catcagatgc gatggtaaat accgcatcgg ataagggacg aatatatttg
    21301 cccatgtatc attccattta tactcgtcat acttcaagtt gcatgtactg cgactcgaat
    21361 tatttagagt atataaatat aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa
    21421 aagaattaat cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac
    21481 ctccttgcag tttgtggaat aagaaaacgc gcacatacta ggcgggcgtt attttcattg
    21541 caagctggat ttaatgttgc ggtttatatc gcatataaaa attagtaaac cacgctcccc
    21601 ggcattcaac gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg
    21661 cggttatgct ggcatctacc gttagttcgg attcagactt aatcaaaggt tttaaca
//
LOCUS       DARNVM010000070        21588 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.070, whole genome shotgun sequence.
ACCESSION   DARNVM010000070 DARNVM010000000
VERSION     DARNVM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.070"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(145..357)
                     /gene="rpmE"
                     /locus_tag="SI621_002442"
     CDS             complement(145..357)
                     /gene="rpmE"
                     /locus_tag="SI621_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEI4065148.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            560..2758
                     /gene="priA"
                     /locus_tag="SI621_002443"
     CDS             560..2758
                     /gene="priA"
                     /locus_tag="SI621_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEI4065149.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAMLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTHRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2914..3939
                     /gene="cytR"
                     /locus_tag="SI621_002444"
     CDS             2914..3939
                     /gene="cytR"
                     /locus_tag="SI621_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HEI4065150.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4031..4990
                     /gene="ftsN"
                     /locus_tag="SI621_002445"
     CDS             4031..4990
                     /gene="ftsN"
                     /locus_tag="SI621_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEI4065151.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5083..5613
                     /gene="hslV"
                     /locus_tag="SI621_002446"
     CDS             5083..5613
                     /gene="hslV"
                     /locus_tag="SI621_002446"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEI4065152.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5623..6954
                     /gene="hslU"
                     /locus_tag="SI621_002447"
     CDS             5623..6954
                     /gene="hslU"
                     /locus_tag="SI621_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEI4065153.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            7021..7947
                     /gene="menA"
                     /locus_tag="SI621_002448"
     CDS             7021..7947
                     /gene="menA"
                     /locus_tag="SI621_002448"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEI4065154.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8040..8525
                     /gene="rraA"
                     /locus_tag="SI621_002449"
     CDS             8040..8525
                     /gene="rraA"
                     /locus_tag="SI621_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEI4065155.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8610..8855)
                     /gene="zapB"
                     /locus_tag="SI621_002450"
     CDS             complement(8610..8855)
                     /gene="zapB"
                     /locus_tag="SI621_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HEI4065156.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9280..10125
                     /gene="glpF"
                     /locus_tag="SI621_002451"
     CDS             9280..10125
                     /gene="glpF"
                     /locus_tag="SI621_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HEI4065157.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10148..11656
                     /gene="glpK"
                     /locus_tag="SI621_002452"
     CDS             10148..11656
                     /gene="glpK"
                     /locus_tag="SI621_002452"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEI4065158.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11791..12801
                     /gene="glpX"
                     /locus_tag="SI621_002453"
     CDS             11791..12801
                     /gene="glpX"
                     /locus_tag="SI621_002453"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEI4065159.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12898..13644
                     /gene="fpr"
                     /locus_tag="SI621_002454"
     CDS             12898..13644
                     /gene="fpr"
                     /locus_tag="SI621_002454"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HEI4065160.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETTAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13649..14077)
                     /gene="uspD"
                     /locus_tag="SI621_002455"
     CDS             complement(13649..14077)
                     /gene="uspD"
                     /locus_tag="SI621_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HEI4065161.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(14104..14403)
                     /locus_tag="SI621_002456"
     CDS             complement(14104..14403)
                     /locus_tag="SI621_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HEI4065162.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14615..15055)
                     /locus_tag="SI621_002457"
     CDS             complement(14615..15055)
                     /locus_tag="SI621_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEI4065163.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTEDVKYKADNKSSN"
     gene            15156..15755
                     /locus_tag="SI621_002458"
     CDS             15156..15755
                     /locus_tag="SI621_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HEI4065164.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15863..16630
                     /gene="tpiA"
                     /locus_tag="SI621_002459"
     CDS             15863..16630
                     /gene="tpiA"
                     /locus_tag="SI621_002459"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEI4065165.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16685..17440)
                     /gene="cdh"
                     /locus_tag="SI621_002460"
     CDS             complement(16685..17440)
                     /gene="cdh"
                     /locus_tag="SI621_002460"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEI4065166.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17547..18536)
                     /gene="sbp"
                     /locus_tag="SI621_002461"
     CDS             complement(17547..18536)
                     /gene="sbp"
                     /locus_tag="SI621_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HEI4065167.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18856..19818)
                     /gene="pfkA"
                     /locus_tag="SI621_002462"
     CDS             complement(18856..19818)
                     /gene="pfkA"
                     /locus_tag="SI621_002462"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEI4065168.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19999..20901)
                     /gene="fieF"
                     /locus_tag="SI621_002463"
     CDS             complement(19999..20901)
                     /gene="fieF"
                     /locus_tag="SI621_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HEI4065169.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21109..21540)
                     /locus_tag="SI621_002464"
     CDS             complement(21109..21540)
                     /locus_tag="SI621_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065170.1"
                     /translation="MKKIQYASPGFIELSALIDISWQIAELVTAVGGSILAANKVYDQ
                     VMRTYRQREWAKLKSEKLRIQNQIKEIELVSDAVKSLESVMALSEEQRKNLVQLSGAD
                     ELVQLKILLAVYRRLSPLVELQNSGKANFSAGKNKNLKASD"
BASE COUNT         5406 a         5586 c         5502 g         5094 t
ORIGIN      
        1 aaataaataa gaaccagaag cctagcgcca gtcaacaaca ggaaatgata atgagttaac
       61 accccggaat ttttcagaat ttataaaagg caaattacag gcacaaaaaa agcgccgtgc
      121 ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg atgttgaaac gcttgttgaa
      181 gcggtcaaca cggccaccgg tagcaacatc acgctgtttg ccagtgaaga acgggtggca
      241 cttgctgcac acgtcgaggt tcaggtcatg accaacggtg gagcggattt tcattacgtt
      301 accgcaagag cagctagcag taatttcttc gtatttcggg tgaatatctt ttttcatggg
      361 aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa cgccagacac cacgcgatgt
      421 taaaagtata gcttcaatac gatcatttcg tacgaaggcg cgaaatcata cagaaattaa
      481 ccagcgtatg caaactgatc cgcactcttc tacggcaatg tgtatactaa cccaccgaat
      541 ttcaagtcag gatgatgcta tgcccgttgc ccacgttgcc ttgcccgttc cgcttccccg
      601 tacctttgac tatctgctgc cagaaggcat gacggtcaaa gccgggtgtc gcgtgcgcgt
      661 gccgtttggc aaacagcagg agcgcatcgg ggttgtggtg tcagttagca atgtcagcga
      721 gctgccgctt aacgagctga aagcggtagt tgaagtgctg gacgttgagc cggtatttac
      781 tcactccgtc tggcgattac tgctctgggc ggcagattac tatcatcatc cgattggcga
      841 tgtgctgttt catgccttgc cgattttact acgccagggg cggcctgcgg cgaacgcgcc
      901 gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg gatctgaaca gcctgaaacg
      961 ctcacccaag caacaacagg cgctggcagc gttacggcaa ggcaaaatct ggcgcgacca
     1021 ggttgccatg ctcgaattta atgatgccgc gttgcaggcg ctgcgcaaaa aaggtctgtg
     1081 tgatttagca agtgaaacac cagagtttag tgactggcga aagaattatg ccgtttctgg
     1141 tgagcggttg cgattgaata ctgaacaggc caccgccgtt ggcgcaattc atagtgcggc
     1201 ggataccttc tctgcctggc tgctggcggg cgttaccggt tccggtaaaa cggaggttta
     1261 tctcagtgta ctggaaaacg tgctcgctca gggcaaacag gcgctggtga tggtgccgga
     1321 aatcggcctg acgccgcaaa ctatcgcccg ttttcgtgaa cgttttaatg cccccgtgga
     1381 agttctgcat tccggcctga acgacagcga gcgtctttcg gcgtggttga aagcgaaaaa
     1441 tggcgaggcg gcgattgtca tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct
     1501 cggcgtgatt gttattgatg aagagcacga cagttcctac aaacagcagg aaggctggcg
     1561 ctatcatgcc cgcgacctgg cggtgtatcg cgcgcatagc gagcaaatcc caattattct
     1621 tggctccgca acgcccgcac tggaaacgtt atgcaacgtg cagcagaaaa aataccgcct
     1681 gctgcgcctg acgcatcggg cggggaatgc gcgtccggca attcaacacg tgctggattt
     1741 aaaaggtcag aaggtgcagg ctggtctggc tccggcgtta atcacccgta tgcgccagca
     1801 tttacaggca aacaatcagg ttatcctctt tcttaaccgc cgtggctttg cgcctgcctt
     1861 gctgtgccac gactgcggct ggattgccga atgtccacgt tgcgatcact actacacgct
     1921 gcatcaggcg caacagcatt tgcgctgcca ccattgcgac agtcagcgtc cggtgccgcg
     1981 ccagtgccct tcctgcggtt ccacgcacct ggtacccgtg gggctgggca ccgaacagct
     2041 tgaacagaca ctcgcgccgt tgttccctgg tgtccccatt tctcgtatcg accgcgatac
     2101 caccagccgc aaaggcgcac tggaacaaca actggcggaa gtacatcgcg gtggcgcgag
     2161 gattttgatc ggcacgcaaa tgctggcgaa aggtcaccac tttccggatg tgacgctggt
     2221 ggcgttactg gacgtagacg gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt
     2281 cgcccagctt tacacccagg tcgccggtcg tgccgggcgt gcgggtaaac agggcgaagt
     2341 ggtgctgcaa acgcaccatc cggaacatcc tctcttgcaa acattgctct ataaaggcta
     2401 cgacgccttt gccgaacagg cgctggctga acgacgcatg atgcaactgc cgccgtggac
     2461 cagccatgtg attgtgcgtg cggaagatca taacaatcag cacgcgccgc tgttcctgca
     2521 acaactgcgt aatctgatcc tctccagccc gctggcagac gacaaactgt gggttctcgg
     2581 tcccgttccg gcactggcac ctaaacgtgg cggccgctgg cgctggcaaa ttttgttgca
     2641 gcacccttcc cgcgtgcgat tacagcacat catcaatggt acgctggcgc ttattaatac
     2701 aataccggat tcccgtaagg tgaaatgggt gctggatgtc gatcccatcg agggctaatg
     2761 cgctcacgat gcgaggcgga tcgaaaaatt caatattcat cacacttttc atgaaaattc
     2821 tgtaaccgtt ttcacgcgct atctgctaaa aatgttgccg atgtgaagta aacatggaag
     2881 tagtacgcct gacgtgccag gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc
     2941 gcgaccatga aagacgttgc cctcaaggca aaagtctcta cagcgactgt ctcccgagca
     3001 ttaatgaatc ccgataaagt ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg
     3061 gaagtgggtt atttaccgca gcctatgggg cgcaacgtca agcgtaatga atcccgcacc
     3121 attctggtga ttgtcccgga tatctgcgat cccttcttta gcgaaattat tcgcggtatc
     3181 gaagttacgg cggcaaatca cggatatctg gtgctgattg gcgactgtgc gcatcaaaat
     3241 cagcaggaaa aaacctttat cgatttgatc atcaccaagc aaattgatgg catgttgttg
     3301 ctgggttcaa ggctgccgtt tgatgccagc attgaggaac agcgtaatct gccgccgatg
     3361 gtgatggcga acgaatttgc accggaactg gagctgccta ccgttcatat cgacaatctg
     3421 accgccgcat ttgatgcagt aaattattta tatgagcaag ggcataaacg gattggctgt
     3481 atagccggtc ccgaagagat gccgctgtgt cactaccgcc tgcaaggcta tgttcaggcg
     3541 ctgcgtcgct gcggcattat ggttgatccg caatacatcg cccgtggcga cttcaccttc
     3601 gaagccggaa gcagagcaat gcaacagctg cttgatcttc cacaaccgcc tactgctgtc
     3661 ttctgccata gcgatgtgat ggcgctcggc gcactttctc aggcaaaacg tcaggggctg
     3721 aaagtcccgg aagacctttc cataatcggt tttgataaca tcgacctgac gcaattttgt
     3781 gatccgccgc tgacaaccat cgcgcagccg cgttacgaaa tcggtcggga agctatgctg
     3841 ttattgcttg atcaaatgca ggggcaacac gttggcagtg gctctcgttt aatggactgc
     3901 gaacttatca tccggggatc aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg
     3961 tgactggtca aaggcccgcc gcttaagtaa catggcgggc tgacgaacga ataaatacag
     4021 cgaaacgata gtggcacaac gagattatgt acgccgcagc caaccggcac cttcgcggcg
     4081 aaaaaagagc acctcacgga aaaagcaacg aaatctgcct gcggtttctc ccgctatggt
     4141 cgctattgct gccgccgttc ttgtgacctt tatcggtggt ctgtacttca ttacgcatca
     4201 caagaaagaa gagtccgaga cgctgcaaag ccagaaagtg accggaaacg gactaccacc
     4261 aaaaccagaa gaacgctggc gctacattaa agagctggaa agtcgccagc cgggtgtgcg
     4321 tgcgcccaca gaaccttctg ccggtggtga agtgaaaacg ccggagcaac tgacaccaga
     4381 acaacgccag cttcttgaac aaatgcaggc tgatatgcgc cagcagccaa cgcagctggt
     4441 tgaagttccg tggaatgagc agacgccgga acagcgtcag caaacgctac agcgccagcg
     4501 tcaggcgcag caattagcgg agcagcaacg cctggctcag cagtcgcgta cgactgaaca
     4561 aagctggcag cagcagacgc gtacgtcgca agccgcaccg gtacaggcgc aaccgcgcca
     4621 gtctaaaccg gcttctactc agcagccgta ccaggatctg ctgcaaactc ctgcgcacac
     4681 gaccgcgcag tcgaagccgc agcaagctgc gccggttgct cgtgccgctg acgcgccaaa
     4741 accgacggcg gagaaaaaag acgaacgccg ctggatggtg cagtgcggtt cgttcagagg
     4801 cgcggaacag gcagagacgg tacgtgctca gctggcgttt gaaggctttg actcgaagat
     4861 caccaccaac aatggctgga atcgtgtggt cattggcccg gtgaaaggca aagagaacgc
     4921 agacagcacc ctcaatcgct tgaagatggc gggtcataca aactgcattc ggctcgccgc
     4981 cgggggttga aaccctcaaa atccccccca tctataattg cattatgccc cgtacttttg
     5041 tacggggttt gtactctgta ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc
     5101 gtacgccgta acggccatgt ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc
     5161 gtaatgaaag gcaacgtgaa aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc
     5221 tttgcgggcg gtactgcgga tgcttttacg ctgttcgaac tgtttgaacg taaactggaa
     5281 atgcatcagg gccatctggt caaagccgcc gttgagctgg cgaaagactg gcgtaccgat
     5341 cgcatgctgc gcaaacttga agcactgctg gcagtcgcgg atgaaaccgc ttcgcttatc
     5401 atcaccggta acggtgacgt ggtgcagcca gaaaacgatc ttattgctat cggctccggc
     5461 ggcccttacg cccaggctgc ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt
     5521 gaaattgctg aaaaggcgtt ggatattgca ggcgacattt gcatctatac caaccatttc
     5581 cacaccatcg aagaattaag ctacaaagcg taaggatctc ccatgtctga aatgacccca
     5641 cgcgaaatcg tcagcgaact ggataagcac atcatcggcc aggacaacgc caagcgttct
     5701 gtggcgattg ctctgcgtaa ccgctggcgt cgcatgcagc tcaacgaaga gctacgccat
     5761 gaagtgaccc cgaaaaatat cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc
     5821 gcccgtcgtc tggctaagct ggcgaatgcg ccgttcatca aagttgaagc gaccaaattc
     5881 accgaagtgg gctatgtcgg taaggaagtg gattctatta ttcgcgatct gaccgatgcc
     5941 gccgtgaaaa tggtacgcgt ccaggctatc gagaaaaacc gttatcgcgc tgaagaactg
     6001 gcagaagaac gtattctcga cgtgctgatc ccacctgcta aaaacaactg gggacagacc
     6061 gaacagcagc aggaaccgtc cgctgctcgt caggcattcc gcaagaaact gcgtgaaggc
     6121 cagcttgatg acaaagaaat cgagatcgat cttgccgcag caccgatggg cgttgaaatt
     6181 atggctcctc cgggcatgga agagatgacc agccagctgc agtccatgtt ccagaacctg
     6241 ggcggccaga agcaaaaagc gcgtaagctg aaaatcaaag acgccatgaa gctgctgatt
     6301 gaagaagaag cggcgaaact ggtgaatccg gaagagctga agcaagacgc tatcgacgct
     6361 gttgagcagc acgggatcgt gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag
     6421 tcttccggtc cggatgtttc tcgtgaaggt gttcagcgtg acctgctgcc gctggtagaa
     6481 ggttgcaccg tttccaccaa acacgggatg gtcaaaactg accacattct gtttatcgct
     6541 tctggcgcgt tccagattgc gaaaccgtct gacctgatcc cggaactgca aggtcgtctg
     6601 ccaatccgcg ttgagctgca ggcactgacc accagcgact tcgagcgtat tctgaccgag
     6661 ccgaatgcct ctattaccgt gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc
     6721 gagtttaccg actccggtat taagcgcatc gcggaagcgg catggcaggt gaacgaatct
     6781 accgaaaaca tcggtgctcg tcgtttacat accgttctgg agcgtttaat ggaagagatt
     6841 tcctacgacg ccagcgattt aagcggtcaa accatcacta ttgacgcaga ttatgtgagc
     6901 aaacatctgg atgcgttggt ggcagatgaa gatctgagcc gttttatcct ataatcgcgt
     6961 tcaatcattt tcatcattgt ttgatggggc tgaaaggccc catttttatt ggcgcgtatt
     7021 atgactgaac aacaaattag ccgaactcag gcgtggctgg aaagtttacg acctaaaacc
     7081 ctccccctcg cctttgctgc aattatcgtc gggacggcgc tggcatggtg gcaaggtcac
     7141 ttcgatccgc tggtcgccct gctggcactg attaccgccg ggctattaca gatcctttct
     7201 aacctcgcca atgattacgg cgatgcggta aaaggcagcg ataaacctga ccgtatcgga
     7261 ccgctacgcg gcatgcaaaa aggggtcatt acccagcaag agatgaaacg ggcgctcatt
     7321 attaccgtcg tgctcatctg tctctccggg ctggcactgg ttgcagtggc gtgccatacg
     7381 ctggccgatt ttgtcggctt cctgattctt ggcgggttgt cgatcattgc cgctatcacc
     7441 tacaccgtgg gcaatcgtcc ttatggttat atcggtctgg gtgatatttc cgtactggtt
     7501 ttctttggct ggttgagcgt catgggcagc tggtatttac aggctcatac attgattccg
     7561 gcactgatcc ttccggcgac cgcatgcggc ctgctggcaa cggcagtact gaacattaat
     7621 aacctgcgtg atatcaatag cgaccgcgaa aatggcaaaa acacgctggt ggtgcgctta
     7681 ggtgaagtga acgcgcgtcg ttatcatgcc tgcctgctga tgggctctct ggtgtgcctg
     7741 gcgctgttta atctcttttc gctgcatagc ctgtggggct ggctgttcct gctggcggca
     7801 ccattactgg tgaagcaagc ccgttatgtg atgcgggaaa tggacccggt ggcgatgcga
     7861 ccgatgctgg aacgcactgt caagggagcg ttactgacta acctgctgtt tgttttaggg
     7921 atattcctaa gccagtgggc agcataactg acaaatatca attaacaatt gatgattttg
     7981 ccaacagcct acatagcgcg atatactgaa aattctcgca gcaactgaat gttaagccta
     8041 tgaaatacga tacttccgag ctttgtgaca tctatcaaga agatgttaac gtcgtggaac
     8101 cgctgttctc caactttggc ggacgggcgt cgtttggcgg acaaataatc acggtaaaat
     8161 gtttcgagga caacgggttg ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc
     8221 ttgtcgtcga tggcggtggt tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc
     8281 tggcagtaca aaatgaatgg gaaggtctgg tcatttacgg cgcggtgcgt caggtagatg
     8341 acctggaaga gttggatatc ggcatccagg cgatggcggc aattccggtt ggtgctgctg
     8401 gcgaaggtat tggcgaaagc gatgtccgcg tcaattttgg cggtgtcacc ttcttctccg
     8461 gcgaccatct ttatgccgac aataccggga ttattctttc agaagatccg ttggatattg
     8521 aatgatagaa aaggcaccgc aaggtgcctt ttttctgcgt tacctgttgg cctacacagt
     8581 aaagaaatta cgcggaagat gaagcgtaat cagacctctt ccatgcgacc cagcagggcc
     8641 tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc acgctccagc
     8701 tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt tttttctttc
     8761 agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg tacttttgct
     8821 tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg caaggcgttg
     8881 atggataaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     8941 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9001 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9061 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9121 tggcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9181 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9241 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9301 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggggtgggt
     9361 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9421 tggggactgg gagtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9481 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg taaagttatt
     9541 ccttttatcg tttcacaagt tgccggcgct ttctgcgctg cggctttagt ttacgggctt
     9601 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagcgttgaa
     9661 agtgttgatc tggctggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9721 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9781 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
     9841 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
     9901 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt tactggcggc
     9961 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10021 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10081 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10141 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10201 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10261 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10321 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10381 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10441 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10501 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttctc
    10561 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgaac gtgcgcgtcg
    10621 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10681 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10741 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10801 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    10861 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    10921 agaagggatg gcgaagaata cctacggcac tggctgcttt atgctgatga acaccggcga
    10981 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgtggcc caactggcga
    11041 agtaaactat gcgctggaag gtgcggtgtt tatggcgggc gcatccattc agtggctgcg
    11101 cgatgagatg aagttgatta acgacgcgta cgattccgaa tatttcgcca ccaaagtgca
    11161 aaacaccaat ggtgtatatg tggttccggc atttaccggg ctgggtgcgc catactggga
    11221 tccgtatgcg cgcggggcga ttttcggctt aacgcgtgga gtgaacgcta accacattat
    11281 ccgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtactggaag caatgcaggc
    11341 cgactccggt attcgtctgc acgcgctgcg cgtggatggc ggtgcagtag caaacaattt
    11401 cctgatgcag ttccagtccg atattctcgg tacccgcgtt gagcgcccgg aagtgcgcga
    11461 agttaccgca ttgggtgcgg cctatcttgc tggtctggcg gttggcttct ggcagaatct
    11521 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11581 tgagcgtaat taccgttacg caggctggaa aaaagcggtc aaacgcgcga tggcgtggga
    11641 agaacacgac gagtaatgta aatgccggat gaagcgttta tgccgcatcc ggtagtcctg
    11701 aatcgtgcgg gggcaacccc gcacacatca ataatcccgc ccttcccctg tgctacactt
    11761 cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat cgaattttcc
    11821 cgcgtcaccg aatctgcggc gctggctggc tacaaatggt taggacgcgg cgataaaaac
    11881 accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt caacattgac
    11941 ggcaccatcg tcattggtga aggtgaaata gacgaagcac cgatgctcta cattggtgaa
    12001 aaagtcggta ctggtcgcgg cgacgcggta gatattgctg ttgatccgat tgaaggcacg
    12061 cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg cgataaaggc
    12121 tgtttcctca acgcgccaga tatgtatatg gagaagctga tcgtcggacc gggagccaaa
    12181 ggcaccattg atctgaacct gccgctggcg gataacctgc gcaatgtagc ggcggcgctc
    12241 ggcaaaccgt tgagcgaact gacggtaacg attctggcta aacctcgcca cgatgccgtt
    12301 atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg
    12361 gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg tattggtggc
    12421 gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat tagatggtga catgaacggt
    12481 cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag
    12541 caggagctgg cacgctgcaa agcaatgggc atcgaagccg gtaaagtatt acgtctgggc
    12601 gatatggcac gcagcgataa cgtcatcttt tctgccaccg gtattaccaa aggcgatctg
    12661 ctggaaggca ttagccgcaa aggcaatatc gcgactaccg aaacgctgct gatccgcggc
    12721 aagtcacgca ctattcgccg cattcagtcc atccactatc tggatcgcaa agacccggaa
    12781 atgcaggtgc acattctctg agtgatttga tcgattgagc cttccagtcc ttcgggactg
    12841 gaattttttt gttcggagaa cgaagataag gcaagtcaat caaaacagga gaaaaacatg
    12901 gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc cctgtttagt
    12961 ctcaccgttc acgcccccgt gcttccgttt accgccgggc aatttaccaa gcttggcctt
    13021 gaaatcgacg gtgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc cgataatccc
    13081 gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg actggcggca
    13141 ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt tgtgctggat
    13201 gaagtaccag attgcgaaac gctatggatg ctggcaaccg gtacagcgat tggcccttat
    13261 ttatcgattc tgcaactggg caaagattta gagcgcttca aaaatctggt cctggttcac
    13321 gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact ggaaaaacgc
    13381 tacgaaggca aactgcgcat tcagacggtg gtcagtcggg aaacgacagc ggggtcgctc
    13441 accggacgga taccggcatt aattgaaagt ggtgaactgg aaagcgcggt tggcctgccg
    13501 atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt gcgcgatacg
    13561 caacagttac tgaaagagac ccggcagatg acgaaacatt tacgtcgccg accgggccat
    13621 atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg atgagcaaat
    13681 ccgcactcaa cttattgatc atcccgcgat atgccggcat caaacggttg ataaatgagt
    13741 ggtgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc agcagtgttt
    13801 ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata tttttcgtca
    13861 gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct tctgttgcag
    13921 ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt aacgtcaggt
    13981 gagcgtcatt atgtctggcg agctccaggg ctttattcac cagtaaggca tcttcttcat
    14041 tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc taaagcaact
    14101 ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct ggcatgagaa
    14161 ggtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttat aggccacgct
    14221 ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg tcataaagga
    14281 ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc
    14341 aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta cgacatcttt
    14401 catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat aatgcgccgt
    14461 taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga tattgcttaa
    14521 tgtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa ataacaatcc
    14581 cggaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta ttgtcggctt
    14641 tatacttcac atcctcggta tctttaccat atttattttc gccttgtgtg ccaacaaacg
    14701 caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc cccaccgccc
    14761 attgccagac acccggtaaa atcgcccagt tacccgccag cagcatccac gccacaatca
    14821 tcaaaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg
    14881 ctgttggcca gagcaagcac acaaggcaaa acgccgcagt ctgaatatcg agtaaatttt
    14941 tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc caaatccaga
    15001 aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg gtcatgtaag
    15061 ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac agatatcgtt
    15121 ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt
    15181 ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca ccagatacgg
    15241 caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctcc atcagccagt
    15301 ttcgagaaga ctttaacagc cagaatccca gcctgccact gaacgaattt cgtgccatcg
    15361 acagcagtcc cgacaaagcc aatctcactc gtgctgccag taaaattaac gagaacttgt
    15421 atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa atgacctggc
    15481 tacccatcca ggggccagag caaaaagccg cgaaagcgaa agctcaggaa tacatggcag
    15541 cggtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa aaactgcagt
    15601 cgctactaac ggcggggaaa aacaaacgtt attacaccga gaccgaaggt gcactgcgtt
    15661 atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg
    15721 cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat
    15781 gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt cctttattcg
    15841 cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact ggaaactgaa
    15901 cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc tggcaggtgt
    15961 tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg cgaagcgtga
    16021 agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc
    16081 attcaccggt gaaacctctg ctgctatgct gaaagacatc ggcgcacagt acatcatcat
    16141 cggccactct gaacgtcgta cttaccacaa agagtctgac gaactgatcg cgaaaaaatt
    16201 cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc atcggtgaaa ccgaagctga
    16261 aaacgaagcg ggcaaaactg aagaagtttg tgcacgtcag atcgacgcgg tactgaaaac
    16321 tcagggtgct gcagcattcg aaggtgcggt aatcgcttac gaaccagtat gggcaatcgg
    16381 tactggcaaa tcggcaactc cggcgcaggc acaggctgtt cacaaattca tccgtgacca
    16441 catcgctaaa gttgacgcta acatcgctga acaagtgatc attcagtacg gcggctctgt
    16501 aaacgcgtct aacgctgcag aactgtttgc tcagccagac atcgacggtg cgctggttgg
    16561 cggtgcttct ctgaaagctg acgctttcgc agtaatcgtt aaagctgcag aagcggctaa
    16621 acaggcttaa gtctgaaagg tgccggattt catatccggc acttactttc cttaactcct
    16681 cgccttaacg caaaatctca cactgatgat cctggatttc ctcggctgaa gcacggttca
    16741 gcgtcagtag atttcgttgt gtcgccagca atacaaatga gttatcactc tgccgcacca
    16801 tcgccagccc gtagcttccc atatgttccc gcgcctcagg cacttcctct gcaagcatca
    16861 taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg tactcatgcc
    16921 cacgcaaacc acctggcagt ggcaaccagc ggctgctgat gttagccaga ttgttatcca
    16981 gttgttcgcg cacatcagga cgaatacaag agatatgaat atgaaaatgg ttttgcgtac
    17041 gcccggtgcg agagttgatc gccaaagaaa ccgcgcgatc gggaaccggc tgaccgtatt
    17101 ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat
    17161 cggtcaacaa aggactttca gtaccgttaa tacgatacgt tggcatcaac agatattgca
    17221 gtgggccatt aagatctttt aaaaccacgt atccggcatt gggtttgact tccgcacatg
    17281 gcgaaggatt ttgattttgc tgttgattgg gcaaacattc ctcaaggaca atcttacgta
    17341 gtgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc acaacggcga
    17401 taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc ctgtacctca
    17461 aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc gagaaaccac
    17521 cgggtttgat aattatcctg gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg
    17581 ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat
    17641 aacttcagct ttggaaacgc attttcgtac tttttcgcca cctcagcatc gcgcggacgg
    17701 tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt caggtaggct
    17761 tccgccacct ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc
    17821 tctgcgagga tagactcact cggcgtaacg atttcgaatt tatctttccc cagctcattc
    17881 gctgccagca gagcttcgtt ttcccaggca atcagtacat cgccaattcc gcgctcgaca
    17941 aaggtgttag tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc
    18001 cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag
    18061 gctgccaggt agttccagcg cgcgccacca gagcttttcg gattaggcgt gatcaccgaa
    18121 acacccggtt taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc
    18181 aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct
    18241 ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc cagagtgaca
    18301 acatcagctt caataccatt gattaccgac gtcgcttgtt tgcctgaacc accgtgtgac
    18361 tgacgaatca ccacgttatc gccagtttgc tgtttccagt gggcgctgaa tgccttgttg
    18421 tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt
    18481 gccataacgc tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc
    18541 cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac aaaacattaa
    18601 agaataaaaa aagatttgct ataacttgcg ggtatatgtt gagggattaa aaaggcggaa
    18661 gaaatccgcc tcatattgct gacaaagtgc gctttatcga tgccggatgc ggcgcgaacg
    18721 ccttatccgg cctacaaaag tttgcaaatt caataaattg cagaattcat gtaggcctga
    18781 taagcgaagc gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc
    18841 ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg
    18901 acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat
    18961 acctacgcaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg
    19021 ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac
    19081 agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc
    19141 acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt
    19201 gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaactacaa cgaattcaca
    19261 gccaccggca atggccgcag ccagggtcag atcgccacaa taacggccca tcacttccac
    19321 cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac
    19381 ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat
    19441 agtgcccggc agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta
    19501 ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat
    19561 agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt
    19621 accaccacgg ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc
    19681 atacagaccc agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga
    19741 acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac
    19801 accgattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc
    19861 ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt
    19921 cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct
    19981 ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga
    20041 acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc
    20101 tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg
    20161 aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac
    20221 acccggccag gaagtcacga tatcaataat ttcttgccgt tcttcatcag gcaatgcgcg
    20281 atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat
    20341 gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag
    20401 cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg
    20461 caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga aacgagacaa ggataatcgt
    20521 acaaattagc gccacaattg tcacgattac cccgacgcct ggatctgtca tcggtgttgg
    20581 agatatcaga tgttgaatac ccgtcaaaaa gaggaatagt gccgaaccgg agataaacat
    20641 actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc
    20701 ggcaggttgc agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac
    20761 cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat
    20821 tttaatcagc aatagcagtg aagccatcgc cgttgcagca atcgccgccc gactgaccag
    20881 ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag
    20941 caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc
    21001 atgtggggga agacagggat ggtgtcacaa aaaagcacct aacctgatga tataaatgga
    21061 tttatgtcag ccgctgtcca catagcgtcc acatcgttca gcgtccgttt aatcggacgc
    21121 ttttaaattt ttatttttcc ctgcagaaaa attagctttc ccgctgtttt gcaactcaac
    21181 aagaggagat aaccgtcggt aaacagccaa aagtatcttt aactgaacca gttcatctgc
    21241 ccccgatagt tgaaccaagt ttttacgttg ttcttcacta agtgccatca cagattcaag
    21301 tgatttaact gcgtcagaaa caagttcaat ctctttaatt tgattctgta tacgcagctt
    21361 ctctgatttc aactttgccc attctctttg tcgatatgta cgcatcacct gatcgtaaac
    21421 tttgttggca gccagaatac ttccaccaac agcagtaact aactcagcaa tctgccaaga
    21481 gatatcaata agagcagaca attcaataaa gccaggagag gcatactgaa tttttttcac
    21541 tacaggacgt aaatctggtg gagtcgcact gtaagcccct ctaaaaaa
//
LOCUS       DARNVM010000071        21580 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.071, whole genome shotgun sequence.
ACCESSION   DARNVM010000071 DARNVM010000000
VERSION     DARNVM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.071"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..86
                     /locus_tag="SI621_002465"
     CDS             <1..86
                     /locus_tag="SI621_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="with GltIKL is involved in the transport of
                     glutamate and aspartate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEI4065171.1"
                     /translation="VLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            86..760
                     /gene="gltK"
                     /locus_tag="SI621_002466"
     CDS             86..760
                     /gene="gltK"
                     /locus_tag="SI621_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HEI4065172.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            760..1485
                     /gene="gltL"
                     /locus_tag="SI621_002467"
     CDS             760..1485
                     /gene="gltL"
                     /locus_tag="SI621_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HEI4065173.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            1603..2538
                     /gene="rihA"
                     /locus_tag="SI621_002468"
     CDS             1603..2538
                     /gene="rihA"
                     /locus_tag="SI621_002468"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HEI4065174.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNASVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2583..3065)
                     /locus_tag="SI621_002469"
     CDS             complement(2583..3065)
                     /locus_tag="SI621_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HEI4065175.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3300..5882
                     /gene="leuS"
                     /locus_tag="SI621_002470"
     CDS             3300..5882
                     /gene="leuS"
                     /locus_tag="SI621_002470"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEI4065176.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            5897..6478
                     /gene="lptE"
                     /locus_tag="SI621_002471"
     CDS             5897..6478
                     /gene="lptE"
                     /locus_tag="SI621_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEI4065177.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            6478..7509
                     /gene="holA"
                     /locus_tag="SI621_002472"
     CDS             6478..7509
                     /gene="holA"
                     /locus_tag="SI621_002472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEI4065178.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            7511..8152
                     /gene="nadD"
                     /locus_tag="SI621_002473"
     CDS             7511..8152
                     /gene="nadD"
                     /locus_tag="SI621_002473"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEI4065179.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8176..8787
                     /locus_tag="SI621_002474"
     CDS             8176..8787
                     /locus_tag="SI621_002474"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HEI4065180.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9047..9364
                     /gene="rsfS"
                     /locus_tag="SI621_002475"
                     /gene_synonym="rsfA"
     CDS             9047..9364
                     /gene="rsfS"
                     /locus_tag="SI621_002475"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEI4065181.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            9368..9835
                     /gene="rlmH"
                     /locus_tag="SI621_002476"
     CDS             9368..9835
                     /gene="rlmH"
                     /locus_tag="SI621_002476"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEI4065182.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            9866..11767
                     /gene="mrdA"
                     /locus_tag="SI621_002477"
     CDS             9866..11767
                     /gene="mrdA"
                     /locus_tag="SI621_002477"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HEI4065183.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            11770..12882
                     /gene="mrdB"
                     /locus_tag="SI621_002478"
                     /gene_synonym="rodA"
     CDS             11770..12882
                     /gene="mrdB"
                     /locus_tag="SI621_002478"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HEI4065184.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            12893..13981
                     /gene="rlpA"
                     /locus_tag="SI621_002479"
     CDS             12893..13981
                     /gene="rlpA"
                     /locus_tag="SI621_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HEI4065185.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14121..15332
                     /gene="dacA"
                     /locus_tag="SI621_002480"
     CDS             14121..15332
                     /gene="dacA"
                     /locus_tag="SI621_002480"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HEI4065186.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            15442..15705
                     /locus_tag="SI621_002481"
     CDS             15442..15705
                     /locus_tag="SI621_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEI4065187.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            15806..16447
                     /gene="lipB"
                     /locus_tag="SI621_002482"
     CDS             15806..16447
                     /gene="lipB"
                     /locus_tag="SI621_002482"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEI4065188.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            16706..17659
                     /locus_tag="SI621_002483"
     CDS             16706..17659
                     /locus_tag="SI621_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEI4065189.1"
                     /translation="MDSNNQIETCLSRKSSEDKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            17868..18833
                     /gene="lipA"
                     /locus_tag="SI621_002484"
     CDS             17868..18833
                     /gene="lipA"
                     /locus_tag="SI621_002484"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEI4065190.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(18934..19137)
                     /gene="tatE"
                     /locus_tag="SI621_002485"
     CDS             complement(18934..19137)
                     /gene="tatE"
                     /locus_tag="SI621_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HEI4065191.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19265..20053)
                     /locus_tag="SI621_002486"
     CDS             complement(19265..20053)
                     /locus_tag="SI621_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HEI4065192.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20149..20532
                     /gene="crcB"
                     /locus_tag="SI621_002487"
     CDS             20149..20532
                     /gene="crcB"
                     /locus_tag="SI621_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEI4065193.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(20586..20795)
                     /gene="cspE"
                     /locus_tag="SI621_002488"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(20586..20795)
                     /gene="cspE"
                     /locus_tag="SI621_002488"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HEI4065194.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(20970..21530)
                     /gene="pagP"
                     /locus_tag="SI621_002489"
     CDS             complement(20970..21530)
                     /gene="pagP"
                     /locus_tag="SI621_002489"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HEI4065195.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTIGENITQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5331 a         5485 c         5462 g         5302 t
ORIGIN      
        1 atgtattgat taacgctttt atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc
       61 tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca ttgtcccttc
      121 cctgccatat ctgctggatg ggctggtgat caccctgaaa atcaccgtca cggcagtggt
      181 gattggtatt ttatggggca ccatgctggc ggtgatgcgc ttatccagct ttgcgcccgt
      241 agcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag ttatggtttt
      301 gctgtggttt tacctgatcg tgccgggttt tctgcaaaac gtgctgggat tatcgccaaa
      361 aaatgatatc cgtttaattt cggcaatggt ggcgttttcg atgtttgaag cggcctacta
      421 ttcagagatt atccgtgccg gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct
      481 ggcgttgggg atgactcact ggcagtcgat gaaactgatt attctgccgc aggctttccg
      541 cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata cctcactggt
      601 gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg agcgtgatgg
      661 tacgcaggtt gagatgatcc tgtttgccgg atttgtttat ttcgttatta gccttagcgc
      721 gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct gaaaaatgtt
      781 tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt gaaaaaaggc
      841 gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat caacgctgat taaaaccgtc
      901 aacggcctcg aaccggtgca gcaaggtgaa attaccgtcg atggcatcgt ggttaacgat
      961 aagaaaaccg atctggcaaa gctgcgttcc cgcgtcggga tggtgttcca gcatttcgag
     1021 ttgttccccc atttgtcgat tattgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa
     1081 cgcgataaag cacccgctcg tgaaaaagca ttgaaattgc tggagcgcgt gggactttcc
     1141 gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcaacagcg tgtggcgatc
     1201 gctcgcgcat tgtgtatgga tcctattgcc atgctgttcg acgaaccgac atcggcgctg
     1261 gatccggaga tgatcaacga agtgctggac gtaatggtcg aactggcgaa cgaaggaatg
     1321 accatgatgg tggtgaccca cgaaatgggc tttgcccgta aagtggcgaa tcgggtgatc
     1381 tttatggacg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt cgatgatccg
     1441 aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact ctggcgcgca
     1501 tacccaagtg cgcgccatct cacgctttat cgtttcgctc ctcgtatctg ttacatcctg
     1561 gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact gccaattctg
     1621 ttagattgcg acccaggtca tgatgacgct atcgcaatag ttctcgccct cgcctcacca
     1681 gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga aaaaacctta
     1741 cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg atattccggt ggcaggcggc
     1801 gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg tgaaagcggt
     1861 ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag
     1921 ctgatggcga aaacgctgcg cgaaagtgcg gaacctgtca cgattgtgtc taccggaccg
     1981 caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa aattgctcgt
     2041 atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc tgaatttaat
     2101 atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc ggtggtgatg
     2161 gccggtctgg atgttactca caaagcacaa attcacgttg aggacaccga gcgtttccgc
     2221 gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt cctcgaatat
     2281 cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg caccatcgcc
     2341 tggctgttaa aaccggagtt gtttaccact gttgagcgct gggttggcgt ggaaacacaa
     2401 gggaaatata cccagggtat gacggttgtt gattattatt atctgaccgg caataaaccg
     2461 aatgcctctg taatggtcga tgttgatcgt cagggctttg ttgatttact ggctgatcgt
     2521 ctgaaatttt acgcttaatc actcgccgga tgacatcacg ccatccggca ccactcattt
     2581 cattaaggct cgaacgggcg tctctggaac tggtcatgac cgcatttcgg gcatagcgtc
     2641 agcacttccg gtgtgtagat cgggagatgg aaatgacatt tctcgcagac cagattcccc
     2701 agccctacca cttctccgct gtgataaacc ccgtgatgat tgaggtcctg gaaaacttcg
     2761 cgccattcaa gctgtgtttt atcggtgatg tctgccagtt cctgccacaa gctttcttta
     2821 atcacccgca taaagacgct gtcagactct tctttcaggc tctcttcata gctcatggcg
     2881 aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgacttcggt tcgcgttaac
     2941 tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg
     3001 ccattgcgca ggcgttcgct cagtgacgcg accagttcac ggtaatattg agcaaccttg
     3061 ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc tttgttcctt
     3121 gctttgctaa tacgcccaca aactctgtaa attaatgtat gaataatccg ggcatcattt
     3181 caacgtcgaa tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc tatgcggatc
     3241 tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca ctggctgcca
     3301 tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga
     3361 agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ctgtctatgc
     3421 ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc attggtgacg
     3481 tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct gcagccgatc ggctgggacg
     3541 cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgcaccg gcaccgtgga
     3601 cgtacgacaa catcgcgtac atgaaaaacc agctcaaaat gctgggcttt ggttatgact
     3661 ggagccgcga gctggcaacc tgtacaccgg aatactaccg ttgggaacag aaattcttca
     3721 ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcagtcaac tggtgcccga
     3781 acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata
     3841 ccaaagttga gcgtaaagag atcccgcaat ggtttatcaa aatcaccgct tacgctgacg
     3901 agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa accatgcagc
     3961 gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt caacgttaac gactatgaca
     4021 acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg
     4081 tagcggcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct
     4141 ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcaacg atggagaaaa
     4201 aaggcgtcga tactggcttt aaagcggttc acccattaac gggcgaagaa attcccgttt
     4261 gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg gttccgggtc
     4321 acgaccagcg cgactacgag tttgcctcta aatacggtct gaacatcaaa ccggttatcc
     4381 tggcagctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa aaaggcgttc
     4441 tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac gctatcgccg
     4501 ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg
     4561 gtgtttcccg tcaacgttac tggggcgcgc cgattccgat ggtgacgctg gaagacggta
     4621 ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat gtggtgatgg
     4681 acggtattac cagcccgatt aaagcagatc cggagtgggc aaaaactacc gttaacggta
     4741 tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc tggtactatg
     4801 cgcgctacac ttgcccggag tacaaagaag gtatgctgga ttccaaagcg gctaactact
     4861 ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac ctgctctact
     4921 tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac gaaccagcga
     4981 aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg
     5041 gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag aaaggccgta
     5101 tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg agcaaaatgt
     5161 ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac ggcgcggaca
     5221 ccgttcgttt gtttatgatg tttgcttctc cggctgatat gactctcgaa tggcaggaat
     5281 ccggtgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtt tacgagcaca
     5341 cagcgaaagg tgatgttgcg gcactgaacg tcgatgcact gactgaagat cagaaagcgc
     5401 tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga
     5461 ccttcaacac cgctattgcg gcgattatgg agctgatgaa caaactggcg aaagcaccga
     5521 ccgatggcga gcaggaccgc gccctgatgc aggaagcgct gctggcggta gtccgtatgc
     5581 ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg
     5641 atatcgacaa cgcaccgtgg ccggttgctg acgaaaaagc gatggtggaa gactccacgc
     5701 tggtcgtggt gcaggttaac ggtaaagtcc gtgcaaaaat caccgttccg gtggacgcaa
     5761 cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg
     5821 gcgttaccgt gcgtaaagtg atttacgtac ccggtaaact cctcaatctg gtcgttggct
     5881 aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg cggtgttaat
     5941 caccgccggg tgtggctggc atctacgtga taccacgcag gttccttcca ctatgaaggt
     6001 catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt
     6061 acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg ttccatccct
     6121 gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta acggtcaaac
     6181 agcagagtat cagatgatca tgacggtcaa tgcgaccgtg ttgatccccg gccgtgacat
     6241 ctacccgatt agcgccaaag tcttccgttc gttcttcgat aacccgcaaa tggcgttagc
     6301 gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct
     6361 gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag aacagacgtc
     6421 gaccacaacg gatactccgg caacgcctgc acgcgtctct accacgctgg gtaactgatg
     6481 attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg cgcggcgtat
     6541 cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctat tcgtcaggta
     6601 gctgcggtac aaggattcga agaacaccac actttttcca ttgatcccaa cactgactgg
     6661 aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca aacgctattg
     6721 ctgttgttac ccgaaaacgg accgaatgcg gcgatcaatg aacaacttct cacactcacc
     6781 ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag caaagcgcaa
     6841 gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca
     6901 ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct caacttagaa
     6961 ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct gctggcgctg
     7021 gctcaggcac tggagcgttt atcgctactc tggccagacg gcaaactgac attacctcgc
     7081 gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt tgatgctttg
     7141 ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc aactgcgtct ggaaggcagc
     7201 gaaccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt taacctgaaa
     7261 cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt atggcagaac
     7321 cgccggggca tgatgggcga ggcgttaaat cgcttaagcc agccgcagtt acgtcaggcc
     7381 gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca gtcagtgtgg
     7441 gcagagctgg aagggttatc tcttctgttg tgccataaac ccctggcgga cgtatttatc
     7501 gacggttgat atgaaatctt tacaggctct gtttggtggc acctttgatc cggtgcacta
     7561 tggacatctt aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc gggtcacaat
     7621 catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa
     7681 acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg aacgcgagct
     7741 aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc aggaacaagg
     7801 gccagacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct ttccgacctg
     7861 gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc gtccaggtta
     7921 cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt tgacacataa
     7981 cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa cgccgtggtt
     8041 taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt
     8101 attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct gatacctgtc
     8161 gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacgca agcgaatatc
     8221 gatggtctgt acagcggtca tgctcccaca cccctgactg cgcgcggtat tgagcaagcg
     8281 caaaatctgc atacgctgct acatggtgtt tcctttgacc tggttttatg cagtgaactg
     8341 gaacgggcac agcataccgc gcgacgggtt ctcagtgatc gccagcttcc cgtgcaaatc
     8401 atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca tcgcgacctc
     8461 atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca tgctatcccc
     8521 acgaacggtg aaggatttca ggcattttca caacgtgtgg aacggtttat cgcaaggctt
     8581 agtgaatttc agcactatca gaatatttta gtcgtcagcc atcagggtgt actgagtctg
     8641 ttaatcgccc gtttaattgg tatgcctgcc gaagctatgt ggcattttcg cgttgaccag
     8701 gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc
     8761 cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa gccgcaacga
     8821 ccattgacag ccccgggcag gctgatattc tccgcagcca gacttttttc gccagacacg
     8881 actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg gtgggatgat
     8941 agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct ggttcaggta
     9001 tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc agggtaaagc
     9061 actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt
     9121 agacgttcag ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg gtacgtccag
     9181 ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt
     9241 accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt
     9301 gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa aactctggag
     9361 ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact gggtacaaac
     9421 cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc tgattgaaat
     9481 tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca aagagggtga
     9541 gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc
     9601 ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aaactggatg gtcgcgacgt
     9661 tagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca
     9721 gagctggtcg ctgtcggcgc ttacccttcc ccatccgctg gttcgcgtgc tggtcgcaga
     9781 gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg agtgataagg
     9841 gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg actatacggc
     9901 agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt tgctgctgac
     9961 cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg attaccagac
    10021 ccgctctaat gaaaaccgca ttaaactggt gcctatcgcg cccagccgcg gcattatcta
    10081 cgaccgtaac ggtatccctc tggcacttaa ccgcactatc taccagatag agatgatgcc
    10141 ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac
    10201 cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt tcacctccat
    10261 tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca atcagtaccg
    10321 ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg gttcggcgtt
    10381 gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa
    10441 tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg gcattgagcg
    10501 ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag ttaacaaccg
    10561 tgggcgtgtt atccgccagt taaaagaagt gccaccgcaa gccggacacg atatttacct
    10621 gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta gccgcgcagc
    10681 ggtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta
    10741 tgacccaaac ctatttgttg acggtatctc cagcaaagat tattccgcct tgctgaacga
    10801 cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg cgtctacagt
    10861 taaaccctat gtggcggttt cggcattgag cgccggagtg atcacgcgca atacgacgct
    10921 gtttgaccca ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa
    10981 atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg ataccttctt
    11041 ctatcaggtg gcctacgata tgggaatcga tcgcctctcc gaatggatgg gtaaattcgg
    11101 ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca tgcctacccg
    11161 cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg
    11221 tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac tgatgatcct
    11281 gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg aagacggcaa
    11341 acaggtgcca tgggtacaac cgcatgaacc gcccgtcggt gatattcatt ccggttactg
    11401 ggagctggca aaagacggta tgtacggtgt tgctaaccgc cctaacggta cggcgcataa
    11461 atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtacagctc aggtcttcgg
    11521 tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac gtgaccacaa
    11581 actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca tgattctgga
    11641 gaacggcggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg accacattat
    11701 gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg cagcggagga
    11761 ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt ccatctcgat
    11821 cccacaatgc tgctgatctt actggcattg ctggtttaca gcgccctggt tatctggagc
    11881 gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc gatgggtctg
    11941 gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg ggccccctat
    12001 ctctatatca tctgtattat tttgctggtg gcagtagatg cttttggtgc catctctaaa
    12061 ggcgctcaac gctggctgga cctcggtatc gttcgttttc agccgtcgga aattgccaaa
    12121 atagccgtac cactgatggt agcgcgcttt atcaaccgcg acgtttgccc gccatcgttg
    12181 aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt ggctgcacag
    12241 cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact gttcctctct
    12301 ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tggcggcgtt cattcctatt
    12361 ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct cctggacccg
    12421 gaatcagacc cactcggcgc gggctatcat attattcagt ctaaaattgc tattggctcc
    12481 ggcggattac gcggcaaagg ctggctgcac ggtactcagt cacagcttga atttctcccc
    12541 gaacgccata ccgactttat cttcgcggta ctggcggaag agctgggatt agtgggcatt
    12601 ttgattctgc tcgctcttta cattctgctg atcatgcgcg ggctgtggat agccgccaga
    12661 gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt attcgtttat
    12721 gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt tccgctccca
    12781 ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg
    12841 tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa
    12901 gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg
    12961 tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccca tagttgaaat
    13021 tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg attaccagcg
    13081 cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg cgggactggc
    13141 ggcaatctat gatgccgaac ccggcagtaa cctgacggcc tctggcgaag cattcgatcc
    13201 gacacagctg acagcggccc atccaacgct tccgatcccc agctacgcca gaattactaa
    13261 cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt acggcaacga
    13321 ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa
    13381 agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc ctggtatggc
    13441 ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt taagcggcgg
    13501 cgcaggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca gtaattcgac
    13561 gctaaaaagc gaagatccga ctggcgcgcc ggtaaccagc agcggtttcc tcggcgcacc
    13621 aacgacctta gcgcctggtg ttctggaagg cagcgaacca acgcctgcac cacagcccgt
    13681 tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggttacac cgcaagccgc
    13741 ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgttagcg atcaggctcg
    13801 tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc gcgtaactca
    13861 aaatggcgca gtctggcgga tccagcttgg cccattcgcc agcaaagccg aagccagtac
    13921 cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca ccgcgcagta
    13981 gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga
    14041 aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt aattccatca
    14101 cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg
    14161 cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata
    14221 tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact
    14281 ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc
    14341 tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag
    14401 aaactgattt agtcaccatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag
    14461 gttcttcact gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg
    14521 gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta
    14581 gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc ctgaaaaata
    14641 cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata
    14701 tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg atctataaag
    14761 aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggtctg ttatgggata
    14821 acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg
    14881 ttgcttctgc gactgaaggc cagatgcgct tgatctctgc ggtgatgggc ggacgtactt
    14941 ttaaaggccg tgaagccgaa agtaaaaaac tgctgacctg gggcttccgt ttcttcgaaa
    15001 ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt
    15061 ctgatcgcgc ttcgttaggt gttgataaag acgtgtatct gaccattccg cgtggccgca
    15121 tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa
    15181 agaatcaggt cgtcggtact atcaacttcc agcttgatgg caaaacgatc gaacaacgcc
    15241 cgctggttgt gctgcaagaa atcccggaag gcaacttctt cggcaaaatc attgattaca
    15301 ttaaattaat gttccatcac tggtttggtt aaaaatcgaa cacttgaaag tgtaatttcc
    15361 gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa
    15421 ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact
    15481 ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa
    15541 gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc
    15601 aactaccact cggtatctat cactatcaac gctactcata tcgagcaggt tgaaacactg
    15661 tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta
    15721 cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt
    15781 actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc gccagctcgg
    15841 tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga
    15901 aagtaccctt gatgaaatct ggctggtcga gcactatccg gtatttaccc aagggcaggc
    15961 aggaaaagcg gagcacattt taatgccggg tgatattccg gtgattcaga gcgatcgcgg
    16021 tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa
    16081 acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac
    16141 cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg
    16201 ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct
    16261 ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc
    16321 cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc
    16381 accgcgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac
    16441 cgcttaattg catacatcaa tggcccaatt tacactgggt cattactcat aatacctgct
    16501 aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga aatgcgatgc
    16561 tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg attatggtga
    16621 gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttaa tattattttt
    16681 ccatgaataa aattggagta agtgcgtgga tagtaataat caaattgaaa cctgtttaag
    16741 cagaaagtcg tcagaagata aaccacaaat atttacaacc ttacgaaata ttgatcttaa
    16801 ccttctgact atttttgaag ctgtatatgt acataaaggg atcgttaatg cagcgaaagt
    16861 gcttaatctg acaccctcgg caatcagtca gtctattcag aaactgcgcg ttatattccc
    16921 tgacccattg tttattcgca aaggccaggg tgtcactcct accgcatttg cgatgcatct
    16981 acatgagtat atcagtcagg gccttgagtc cattcttggc gcgctggata tcgaaggaag
    17041 ctatgataag caacgaacga taaccattgc gaccaccccc tcggtcggag ccctggtcct
    17101 tcctgtcatc tatcgggcga ttaaatctca ctatccgcag cttttactgc gcaacccccc
    17161 catcagcgac gcggaaaacc aactcagtca gtttcaaacc gatctcatca tcgataacat
    17221 gttttgcacc aatcgtacgg tgcaacatca tgttctgttc accgacaata tggtgttaat
    17281 ttgccgtgag ggaaatccac tactctcttt agaagatgac agggagacta tcgacaacgc
    17341 tgcgcatata ctcctgttac cggaagggca aaatttcagc ggtctgcggc agagagttca
    17401 agagatgttt ccggaccggc aaatcagttt caccagctac aacattttga caatcgctgc
    17461 actggttgcc aacagtgaca tgttagcgat tattcccagc cgtttttata acctgtttag
    17521 ccgttgttgg ccgctggaaa aactgccttt tccgtcctta aatgaggagc aaatagattt
    17581 ctctatccac tacaacaaat tcagcctgcg tgatcccatc ctgcacgggg taatagatgt
    17641 tatccgtaat gcattttgaa aaagtattgt tacagccaga tcacagcgaa gagcgaaaaa
    17701 atggcacaat ccaacaacaa ttttacattt tcgcgaccgc tttggctgct ttcaggtccg
    17761 tttcaatgat atactgccag tcgttaattc aaaaatagtt gataattaca acaatctatt
    17821 gaattgaaac gctttccttc gtaattcgca actggaacac gcacgctatg agtaaaccca
    17881 ttgtgatgga acgcggtgtt aaataccgcg atgccgataa gatggccctt atcccggtta
    17941 aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc
    18001 ttccagcgga ctctacacgt atccagggca tcaaagccgc aatgcgcaaa aatggcctgc
    18061 attctgtctg cgaggaagcc tcctgcccta acctggcgga atgcttcaac cacggcacag
    18121 caacgtttat gatcctcggc gctatttgta cccgccgttg tccgttctgt gacgttgccc
    18181 acggtcgccc ggtagctccg gatgccaatg aaccagtgaa actggcgcag accattgccg
    18241 atatggcgct gcgttatgtg gttatcacct ccgttgaccg tgatgacctg cgcgatggcg
    18301 gtgcccagca ctttgcggat tgcattactg ccattcggga aaaaagcccg caaatcaaaa
    18361 ttgaaactct ggtgccggat ttccgcggtc gtatggatcg tgctctggat attctgaccg
    18421 ccacaccacc agatgtgttc aaccataacc tggaaaacgt accgcgtatt taccgtcagg
    18481 tacggcctgg tgcagattac aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc
    18541 atccggaaat cccgaccaag tctggtctga tggtgggtct gggtgaaacc aatgaagaaa
    18601 ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat
    18661 atttgcagcc aagccgccat cacctgccgg ttcaacgtta cgttagcccg gatgagttcg
    18721 acgaaatgaa agccgaagcg ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg
    18781 tccgctcgtc ttaccacgcc gatttgcagg cgaaagggat ggaagttaag taatcctgta
    18841 ataattactt acatctgtct gataaaaaac ccgctttttg aagcgggttt tttgtatcga
    18901 acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga gcttttcagc
    18961 ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca tcgccttctt
    19021 gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct tagtcccaaa
    19081 cagcagaacg accagcgccg caactaccag cagtttggta atactaatct cacccataga
    19141 taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccagaga atattaacgg
    19201 tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg ccgagtaaaa
    19261 aacatcataa taattgcggc ggcgcaaagc gacggttgtt taaaacggga agttgcgcgc
    19321 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    19381 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgcccgata tttttgttcc
    19441 cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    19501 ttgaccagtg atgctctttg agcggcccac gaacccaggc ggcaggaagt accaggattt
    19561 cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    19621 tcagaccgac cttcatccct tccacctcca gtaacggagc tatttcatta ccagcatcaa
    19681 catggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca taacgggcga
    19741 cgatgtttcc tgcctgtagc gccaccagca tattccatgc gcgccccggc gttgaaggaa
    19801 catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaacgtccga
    19861 ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca tgatcatcgc
    19921 gcgccagcaa tgcttccggc aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca
    19981 tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca aactgtcctg
    20041 cggcaaccag cattgccacc tcctcctgtc agagtcgtaa aattcgccaa cggcgattat
    20101 tcggcgttac acttatcact catacaaatc aaatagcagg attttgcagt gttacaactt
    20161 cttttagcag tttttattgg cggtggtacg ggaagcgtgg cgagatggct gttaagtatg
    20221 cgatttaacc cgctgcatca agcgattccg ttggggacgc tggctgcaaa cctgattggg
    20281 gcattcatca taggaatggg attcgcctgg tttagcagga tgacgaacat tgatccagtg
    20341 tggaaagtat taatcaccac cggattttgt ggcggtctaa caaccttctc aacattttcg
    20401 gcagaagtgg tgtttttgtt acaagagggc cgctttggct gggcgttact gaacgttttc
    20461 gtcaaccttt tggggtcttt tgccatgacc gcactggctt tctggctgtt ttccgcctca
    20521 accgcacact aaagaccaaa aaaaacccgc tgattaagcg ggttttgaat tcttgctgac
    20581 gtatcttaca gagcgattac gtttgcagca gaagggcctt tggcaccgtt agtgatttcg
    20641 aactctacgc gctgaccttc agcaagagtt ttaaaaccat tagtctggat tgcagagaag
    20701 tgtacaaaca cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc tttggactca
    20761 ttaaaccact taacgttacc tttaatctta gacatcaaaa ttacctttac atgaaaaata
    20821 gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg tacgctttgt ccagtgcaac
    20881 tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt aattatttta cccattgatt
    20941 ttaaagagtt tactaaaatt tcatttgtct caaaactgaa agcgcatcca ggcaaagtac
    21001 acattgccat tgttataggt acccggaatg taggtcatct gaaaagtcac tggaccataa
    21061 cccacagagg ccaatggcag tagaaccggg agagggatgt aattccagtt atcgcgtgcc
    21121 gttacgccag cggtgaatcc cagacctaaa tgaaaatttt catccgccag cggtcgccag
    21181 gtactttccc atccgtatcc ggcaatcggt tcccatttgt tccacgagtc cttaaatgcc
    21241 atggcataca ggccatgcca gtttcctttt tcatcccatc gcgactgacc aaaaccgcca
    21301 ccccacggtc gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa acgtgcatgc
    21361 caggtgatgg caggaatata taaatcataa tgttcaggct gttgccaggt ttgtgtaata
    21421 ttttctccaa tcgttgtcat ccactcatct gcgttagcaa aaactttacc aacgctgatt
    21481 aactgaataa aaacaaagga aaagatagcg acatatttac tcacgttcat ttgtgaccat
    21541 aaaacattta tcaaaaatct actactagca tagcaaagct
//
LOCUS       DARNVM010000072        21570 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.072, whole genome shotgun sequence.
ACCESSION   DARNVM010000072 DARNVM010000000
VERSION     DARNVM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.072"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            15..91
                     /locus_tag="SI621_002490"
     tRNA            15..91
                     /locus_tag="SI621_002490"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Met,seq:cat)
     gene            complement(166..1419)
                     /gene="amiC"
                     /locus_tag="SI621_002491"
     CDS             complement(166..1419)
                     /gene="amiC"
                     /locus_tag="SI621_002491"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HEI4065196.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1651..2982
                     /gene="argA"
                     /locus_tag="SI621_002492"
     CDS             1651..2982
                     /gene="argA"
                     /locus_tag="SI621_002492"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEI4065197.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3044..4870)
                     /gene="recD"
                     /locus_tag="SI621_002493"
     CDS             complement(3044..4870)
                     /gene="recD"
                     /locus_tag="SI621_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEI4065198.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFSD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4870..8412)
                     /gene="recB"
                     /locus_tag="SI621_002494"
     CDS             complement(4870..8412)
                     /gene="recB"
                     /locus_tag="SI621_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEI4065199.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSICDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8405..11293)
                     /gene="ptrA"
                     /locus_tag="SI621_002495"
     CDS             complement(8405..11293)
                     /gene="ptrA"
                     /locus_tag="SI621_002495"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HEI4065200.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFDRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11469..14837)
                     /gene="recC"
                     /locus_tag="SI621_002496"
     CDS             complement(11469..14837)
                     /gene="recC"
                     /locus_tag="SI621_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEI4065201.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENCAVAGVNIEEFSRSDNKRLLDPLDNSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14850..15173)
                     /locus_tag="SI621_002497"
     CDS             complement(14850..15173)
                     /locus_tag="SI621_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEI4065202.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPAHWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15158..15565)
                     /locus_tag="SI621_002498"
     CDS             complement(15158..15565)
                     /locus_tag="SI621_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HEI4065203.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15562..16125)
                     /locus_tag="SI621_002499"
     CDS             complement(15562..16125)
                     /locus_tag="SI621_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HEI4065204.1"
                     /translation="MPVKELGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16116..16586)
                     /locus_tag="SI621_002500"
     CDS             complement(16116..16586)
                     /locus_tag="SI621_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HEI4065205.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16771..17565)
                     /gene="thyA"
                     /locus_tag="SI621_002501"
     CDS             complement(16771..17565)
                     /gene="thyA"
                     /locus_tag="SI621_002501"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEI4065206.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17572..18447)
                     /gene="lgt"
                     /locus_tag="SI621_002502"
     CDS             complement(17572..18447)
                     /gene="lgt"
                     /locus_tag="SI621_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEI4065207.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18598..20844)
                     /gene="ptsP"
                     /locus_tag="SI621_002503"
     CDS             complement(18598..20844)
                     /gene="ptsP"
                     /locus_tag="SI621_002503"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEI4065208.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20857..21387)
                     /gene="rppH"
                     /locus_tag="SI621_002504"
     CDS             complement(20857..21387)
                     /gene="rppH"
                     /locus_tag="SI621_002504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEI4065209.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         5125 a         6009 c         5399 g         5037 t
ORIGIN      
        1 aagcagtacg gtgacgcggg gtggagcagc ctggtagctc gtcgggctca taacccgaag
       61 gtcgtcggtt caaatccggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt
      121 tgtttctgtt ctcccataaa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc
      181 cagcgtcgcc ccatcggcaa aatacgcttt aatccccgca agaatagact ccgcaacttc
      241 ctgctggaaa gtcgccgttt tcagtttacg ttcttcctca acgttactga taaacgccgt
      301 ttcgaccaga atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac
      361 ttgattttta tgcagcttgt tgattttccc gagcttattc agcaccgctt taccaaactt
      421 caggctgtcg gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg
      481 gtcaccgctt ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt gtgccagata
      541 ttttgccgca gtactggttg cacctttggt tgagagcgca aacacagagg aaccgctcgg
      601 ctgacgactg gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg
      661 ggcttttgct acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac
      721 cttcatattg ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac
      781 gtctttttcg cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg
      841 gtcaagcata atgacaatcg gacgatcgcg ccctgcttta cctggttgtg gaccactttg
      901 cgctggcggc acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag
      961 cgggtcctgc atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa
     1021 cccggcgacc ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac
     1081 catgcgtacg gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc
     1141 tgcacgaatt tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc
     1201 caccaccacg cgttccgggt tactcaacgc gaactgctta tatttcagct gacgatttga
     1261 ttctaccgtc acgcgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct
     1321 gaccgcagcc agactgacct gacttacgct caatagccac atggcacccg cgccttgcag
     1381 taaacgacga cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa
     1441 tctaagctgt gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata
     1501 atctgtcatc taataaacgg taaacattct ttttatattc acggcattac tgataaaaaa
     1561 gtcgctctcg cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacacta
     1621 atttcgaata atcatgcaaa gaggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc
     1681 gagggattcc gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc
     1741 atgctcggcg gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg
     1801 ttgttgcaca gcctcggcat ccgtctggtg gtagtctatg gcgcacgtcc gcagatcgac
     1861 gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac
     1921 gccaaaacat tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct
     1981 cgcctgtcga tgagtctcaa taacacgccg ctgcagggcg cgcatatcaa cgtcgttagt
     2041 ggcaatttta ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc
     2101 gggcgtatcc ggcggattga tgaagacgcg atccatcgtc aactggacag cggtgcaata
     2161 gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa
     2221 gagattgcca ctcaactggc catcaaactg aaagctgaaa agatgattgg tttttgctct
     2281 tcccagggcg tcactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg
     2341 caagcacggg tagaagccca ggaagagaaa ggtgattaca actccggtac ggtgcgcttt
     2401 ttgcgtggcg cagtgaaagc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat
     2461 caggaagatg gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt
     2521 gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca acgatattgg cggtattctg
     2581 gagttgattc gcccgctgga gcagcaaggt attctggtgc gccgttctcg cgagcagctg
     2641 gagatggaaa tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc
     2701 gcgctctatc cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg
     2761 gattaccgca gttcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag
     2821 cagagcggct taagcaaatt atttgtgctg accacgcgca gtattcactg gttccaggaa
     2881 cgtggattta ccccagtgga tattgattta ctgcccgaga gcaaaaagca gttgtacaac
     2941 taccagcgta aatccaaagt gttgatggcg gatttggggt aagcgatgaa aatcgccgga
     3001 tgcgacatgc gtaacacccg tgcgtcgcat ccggcaatta cgtttattcc cgcgaactaa
     3061 acaatgccgc cagaccactg cgccgctcag tgcgcgtggc gattgccgca ctcaaaatac
     3121 gctcatcggc atacagcgac aaacgtcgac gcgcgcgggt taccgcagtg taaaccagct
     3181 ctcgtgttac taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg
     3241 atccctgcga tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg
     3301 gttgcacaga cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccac
     3361 gatccagagc gataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa
     3421 tcatcaccgg tcggccttcg taccaacgag agtgcggatt acgatgaatt ttgcgcttct
     3481 gttgcataaa ctgctcaatt cgctcattca gtccagccac gccaaatggc ccttcccgca
     3541 gggcgcacaa aagttggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg
     3601 cttgcagcag atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat
     3661 aatcttcgcc gctctgtaaa agccgttttt cgatatcaga aaaatcctgc tgaaaaacgg
     3721 ttttcactgc cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat
     3781 cgctgccgaa acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt
     3841 ctgtgccagt tcctgccgga acgtgagttc ccgtcaggcg gcttagctgc ccggcacgct
     3901 cggcggtaaa gccggcattg gcataagcac aaatatcgcc cagcacagcc ccagcctcaa
     3961 ccgaggccag ttgatcacga tcgccgagaa agatcactcg cgcatgatcg ggcaaggcgt
     4021 cgatcagtct cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat
     4081 caagatgcag cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgtgcaccca
     4141 gcaatcgatg caaagtactg gcatcttccg gaatgcgttt cttttgttca tcggtcagcg
     4201 gtaactgtcg caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg
     4261 gtgcagccaa acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca
     4321 acttcgctac ggtggtcgtt ttaccggtgc cagggccgcc ggaaatcacc gagatccgcc
     4381 gcgtcagcgc cactgccgcc gcaacttttt gccagttaat ttcatcgctt actggaaaaa
     4441 gtttgtccag ggtttgcgcc agtagagctt catcaacctc aatggcatga ttcacttcgt
     4501 tgaaaaagcg tgccactgtg cgctcgttac accacatgcg attcaaataa agacgatcgc
     4561 cacagaggat catcggcgtt ggttcatctc cccggctgac cgcttgtgaa gccagcaagc
     4621 attcttccca attttgtaac tcaccgattt cactgacaca ggtcgccaac agcggatgcg
     4681 acgcctcgtt attttccagt cgtgagagcg gtaaacagac atgcccttct ccggcgtcgt
     4741 ggcttaacag ggctgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg
     4801 caaattgcac atccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt
     4861 gcaatttcat tacgcctcct ccagggtcat accggcaaac atctcatcca tcaggtcaat
     4921 caacccggcg ttgggtcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacgcc
     4981 acgcaggaac agatagataa cgccgccaaa gtgacgatca tagtcgtaat cagcaatgcg
     5041 atggcgcaga taacggtgca gcgccagggt ataaagctga tattgcagat catagcggtg
     5101 tgcctgcatc gccgctgcca tagcctgttg ggtgtaagcc gaactgtctt cacccagcca
     5161 gttggattta tagtcgagca ggtaataacg cccttcgtgg cggaatacca ggtcgataaa
     5221 gccttttaac atgccgcgta cctgcatgaa ctccagcggc gggcagccag cggatagcgg
     5281 atcaaactgg cgaattagtg catcaagctg gctggcgatg agcggttcac taatcggcag
     5341 gtaaaactcc atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt
     5401 ttcattgaga ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg
     5461 cgattcaaag ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt
     5521 aaaatcgagg tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa
     5581 ctgatgcggt gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc
     5641 cagccgtggc atcagatcct gagcgatacc gtgaccacgc tgctgcaaac cggagtagct
     5701 ggtgacacgc cagttatcgc cgggcaatcg ttgtagtgtc ctcgcattca gttctgcagt
     5761 tatagcatca ttaacctgcc agggctggtt atcacccgtt tgtgccgttt gccaggcaat
     5821 atcatcatcg cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc
     5881 tttttgcagc aaacgcccga gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc
     5941 acgacggcgc accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc
     6001 cacgtaaagc aaacgcagat cttccgccag acgttcggcc tccgcgaggt cgacgctttc
     6061 tggcgcagca ttaagatcca gaacagcctc aaacgagtgg cgatcgtgat agaacgcctg
     6121 atcctggacg cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt
     6181 tgatttatgg atcgtgacaa tctgcaccag atgtttatca ctttcgaggc gcatttgttg
     6241 gctggaggca ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg
     6301 ttcactttcc agctgcgttc cggcttcttg tagcagttcg ctgatatgca agatatcggt
     6361 aagacgccgc tcaccgcctg ccgttgccag caagttttca gcaatgttac gcgccgacat
     6421 cagcgcccgc agcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa
     6481 ctcttcgact accgcatccc acgcatgttc gtcattgttc agcgtttcga tatccagcgc
     6541 gttcagcccc atcattgacg ttgccagcgc actacgcagg gtgttctcac gttcgggcgt
     6601 catcaccgcc tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc
     6661 gcggttcgaa aggtaaacgg aagggatttc cagcaacgtt aaggcgtcgc gcacctgggc
     6721 ggcctcctgg cggctgcgca ccagcacact gatgtccgaa gcacgcaccg gacgcgcgtc
     6781 gtcgccgttc atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcggatttg
     6841 cgcagcacat acctgcgcca tggtactttg atagtcgcca acgccgcagc tttcgccttc
     6901 catcagccac attttcatcg caggctgcgt ttcaccttta aacacaaaac gtaacgcctg
     6961 atttttcccg gctgatttca ctggaataaa aggaatttcg cgaaacatga acgcgtcatc
     7021 agtctgactg aaaagcttat tcacgctgtt caccattcct ggtgcggagc gccagttggt
     7081 atctaaagtg tagtgggcgt gaacttcgct acgcgccttc atataagtga agatatccgc
     7141 accccggaat gcatatatgg cctgtttcgg gtcgccaatc agcaacaatg cggtttccgg
     7201 ctgatggtgc cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc
     7261 atcgatcatt gccaccggga atcgcgtacg gatcgccgct gccaacacct cgccgctttc
     7321 gctacgcagc gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg
     7381 gcgtttttca cgcgctactg tttcgcggat ctcagccaat gcgcgggtta tcagcagatc
     7441 gcagatcgac aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg
     7501 ggtttccccc ccggctttcg tgcgatcttc taagaaacgc tgagagaatt tttccagcga
     7561 ctccggtaac tgataactgt tggtctcttc ttctgcccag gcgctgatct tgtcgatcca
     7621 tttagcctga ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc
     7681 atccagttca cccaccgcgt cgcgccactg ctgttttacg gcgtcgatac gcgccacaat
     7741 ttgcgcgtgg cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggtgc
     7801 ttcgccttgc agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc
     7861 aaagaccacc tgggcaattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc
     7921 gcaggcctgg tagcgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc
     7981 ggattcaaag gcattcaggt tgagcatgcg ctggcaaaaa ccgtgaatag taaagactgc
     8041 cgcttcatcc atctgccgtt cggctaacaa caaccactgc gcggcttgcg ctttatcgtc
     8101 gatctcttcc agcaggcgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat
     8161 gcgcaactca tggatattgc tacggatacg accgcgcaat tctgccgtgg cagcctcggt
     8221 aaaggtgacc accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cagaaccgcc
     8281 tagtccaagt aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc
     8341 agaggcttca atcaggcgct cgccctgtaa gggcaagcgc aaaggatcta gtgtctcggc
     8401 gacatcactc attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct
     8461 cccacacttt ccagccttca ggatgcacat attcggcttt cccgttctgg ctgccagaaa
     8521 tctgcgacag aatagccatg ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa
     8581 gtttttgcgg cgtcagcagt tttatctggg ccacgatttt atcacgcgaa tcgaagcgca
     8641 tattgccgcg atcgaaatct ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg
     8701 cctgcagcat ctgggttatt accgcctgct ggatttgcgc aaactcttct ggcttcatcg
     8761 tccgcaattt tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaatgaag
     8821 gctgtttatc attgctttgc aacaggaagc ccatgcccca ctgacgcccc acgctcattg
     8881 gaaacgcaaa cacggcatag cccaactgtt cttcggtacg caactgattg tagaaccacg
     8941 gctgtacgat ctgccccaac agagagctat aggctgagct ggtgtattca tcgtagccag
     9001 tcggtacaaa taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga
     9061 cggattgttt tttatcgacc acgacatctt tgttacgaca ccactccgaa ccatcagcgc
     9121 ccaactgttt ttgcacatcg cgtgccagcg ttgttgcctg ggcttcggtc atgttgccaa
     9181 taaccataaa ctctggtcga gcccctgatt ttaaggcgtc gcgataggcc agcacctctt
     9241 tcaaagtaat ggagggcaaa attttgcgcc gttcatctcg cgagaagtac ggcacttgcg
     9301 agagcatctg ggcgggcata atcgcctgct cgaacgcttt gcccttttct gcagaatcca
     9361 tcatctggtt ataccaggac ttcgcctgct caagctgatc ttccgtagcg gtatagctaa
     9421 agtacccctc gagcaatgcc tggaacagct gcggcaggcg ctgggtgtaa ccattggcat
     9481 taaccataag gccgttgtta gcattggtgg aaaaacttat gccaccaacc gacgcctggt
     9541 tgcttaactg atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat
     9601 tgcgggcgct gtccatggct ttcgggttac gcaaaatcag gctgacatca gctttgggtt
     9661 cgctggaaaa ataacggctt ggcgcataca ccacacgcag attcgactca tcaacaatca
     9721 gctctggatg gtcatatttc ttctctgact taatcagcga gaaatcgtca ggaatatagg
     9781 ggttaagctc tggcaaagag agcgcaatgt cggcggcttt tttctgccag tcagcgaaag
     9841 tttgtgcgct aattttatcg acctgatacg gcgcatcgac aaagtaagcc gttttgttgt
     9901 gcggctcttt cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccaggc
     9961 gttcttttac tgctttagcg tcgtaccgat cggcaatatt gactgcatcc agcgtatgct
    10021 caacaggaac gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga
    10081 ttgacggata acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat
    10141 caatcccttt ttcgcgtaac agattgaggt agctaaaaat ggccgccaca acctgatcgc
    10201 gattcgccag acctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt
    10261 tgacgatagg atcggaattg gcgctaatgc cctcaactaa tccctgcttt tgcagccagt
    10321 cagaaagtgt acctgggctg cgattaccga tcagataggt aatcaattca tccgttttac
    10381 tacggaactt cgctgaattg ttatcgatgc gaaactcaac gcgcagaact ttacgcggca
    10441 gcgccgggac gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga
    10501 tttccggttt tttgctctct ttgttcggca cgcgatcaaa ggtgtccgcc gccatttttg
    10561 ccaactccgg cagcggttta ttgctgtaaa taaccgcctt catcagattg gcggaatagt
    10621 acttctcgtg gaaatctttc agcgcctgct gtaccggatt tccgggtttg tcgcttaaag
    10681 tttcgaggtt accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc
    10741 tgacctgtgc catgcgcatc ccgtcacgcg tacgcgccat ggttaattcg gcattcactg
    10801 cattacgttc acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcgtccgcca
    10861 ggcggtctac cgcaccgggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat
    10921 acggtgccgt gctggcattg tgactgccgc cgtgcatttt gagatattcg gccagactgt
    10981 cagcctgcgg gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc
    11041 cctggtacgc ctcgggatct tccagcgacc caacgggcac taccagcgcc gagagcgatt
    11101 taaccgcctg cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct
    11161 gatactggcg gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg
    11221 tttctgcctg acttaagggt gcccaaaggg caacaaacaa caataatgct ttgaaccagg
    11281 tgctgcgggg cattcacgga cctcataatc aactttattt tctggccaga ttcaacaggt
    11341 taacgcaaat catctgccaa aatttaatct tgtgctgcac gagtcagcct atgtctatat
    11401 atccatcagt ccgtgactgg tgcgcatcat aaagcaagca gatatattgc gcaattttta
    11461 tacagcactc atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca
    11521 acgatggcct ccattgtctc tggtgttaat tgccgccaga gccgttgata ccagatatca
    11581 tcaccttcgc cacgcaccat catgttgcct tcgtaagcct gaaggaattt cgtacgggct
    11641 ttttgtaacg tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt
    11701 agccacgcgc cgccactttc aggtaacacc agcaatggcg cggacattcc ttcacgatac
    11761 ccctcaatca gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc
    11821 cactcgccgt ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag
    11881 acaaggtgtt ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag
    11941 cgcaacaggc catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcag
    12001 gcgagatcaa tttccatact ctgccccggc tggcgacagg caataactct gtcggcaagc
    12061 tgctgcatct cctggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa
    12121 tcccctgccg cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtaca
    12181 ttcaataact gctgattgat ttgataacgg ctaagccctt ccagaataaa tggctcggtg
    12241 tcggggattt cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc
    12301 cgtaccggat gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta
    12361 aacggcagcg gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc
    12421 cattcacgag catagctttg tcgttcgcct ggctggtagt tttgcggatc aaacggcatc
    12481 cgggtatgga ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc
    12541 gcttcatcgc ccggtagata atggctttgc ccgatgtagt cgatcagttc ctgcaccagt
    12601 accgacggga aacgttcact gttatcctga atagaacgcc cgatatagct gatatagagt
    12661 ttttgctgcg cggaaattaa cgcttccagg aacagatagc ggtcgtcatc gcgacgacta
    12721 cggtcgccac gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctggcgt
    12781 ggataaacgc cgtcgttcat tcccagcagg caaaccactt tgaatgggat ggaacgcatt
    12841 ggcatcagag tacaaatgtt aaccggcccg gcgagaaaac gctggctgat acgttcctga
    12901 tccaggcgct gtgccagttc atcacgcaat agtgacagcg gcaccgcgtc gccatactgc
    12961 gcacctaaac cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct
    13021 tcggtttccg catccggcag gaagaaggcg ttgagcatat cgcgacaaac cggcaaccac
    13081 tcttccagcg gacgctcctg cgccagcccg cgacgccaga tgtttagctg cattagcagt
    13141 gaagccagat gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc
    13201 gattgccact cgccctgcgc actctccatc gcgtagccca gcaacatgcg cgtcaggcca
    13261 aaccgccagg tgtgttgacc ggtagcgggg agttccagct cgcgaacgtt gtcgtcatct
    13321 atcccccaac gaatgccgga ttcgttgacc cactggcgta aataacgcag cccttcttcg
    13381 gtgatgtcaa accgcgccgc cagcaccggc acatccagta atgccagaac gtcctccgac
    13441 acaaagcggc tgtctggcaa cgataacagg ctgataaacg cttcaagtac aggatgcgac
    13501 tgccgcgccc gacggtcgga aatggcgtaa ggtaggtaac gatccgcagg tgcgctacca
    13561 aacactgcct gaataaacgg actgtagctg tcgatgtcgg ccaccatcac gatgatgtcg
    13621 cgcggagtca atgtcgggtc ttcctccagc attgccagca ggcgatcgtg taaaacttca
    13681 acttcacgct gcgggctatg gcaaacgtgg aaggtgatac tgttatccag tggatcaagc
    13741 aggcgtttgt tatcgctacg ggaaaactct tcgatattca cacccgcaac agcgcagttt
    13801 tcaagttcca gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt gacatcgaca
    13861 aaggcgtcca gctcctggct gccctccaga tcagaaagga gataaatgta gtcgcgccca
    13921 agcttgcccc atgaggccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag
    13981 agctgcccgg cattttcgct gtcgcgaaat agcggtaatt cgcgatcttc aaaactgtgt
    14041 cgacgctgac gagtcagcag tttcgccaga taagcaggat ctttaatgtc gccccagtaa
    14101 taacggcagg ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctgtagc
    14161 gcctggagat aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtagc
    14221 cccggggggc aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg
    14281 cggtgccagc gcggttgccc gagttgatgg gtatattcca ccaacgcctt ccacaacggc
    14341 gcttgccaag cctgtgcttc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc
    14401 agccagtccg gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaagaaagc
    14461 tggaacagtt ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct
    14521 tcgcgctcca acagttgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta
    14581 aaggcactct ctttggggat ctccggtaac acccggacga acatatccca tataaagctc
    14641 gctggcagcg gaaaatcaat atttgccgca ataccaaact tttgcgacag ggtcatttgc
    14701 agccactgcg ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc
    14761 agccgttcgc gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa
    14821 tggtagaccc ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc
    14881 atctcgcctt ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgct
    14941 tgcgatgtct gcattcggtt gacctgccag tgggcaggtg gcgaaatcgc acgcagttgc
    15001 gtttgctgcc agccatgccg ccagagctgt tggtactggt ttctactggc aagactgttc
    15061 attaatgttc gctggaaacc cgataacgca gtgacaatca tcaccatcag caccattgcc
    15121 aacatcacct ccgggaggct aaagccttgt tgattcttca aggaagctga cataacgccc
    15181 tctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatg tttccatcga
    15241 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcctcat
    15301 tatctgccaa taaacgcaga caaacccggg caccggtggc agcgtacagt aagcactgaa
    15361 ctgcgggctg cgtctgccag gagtgcattt ttccccactc cagcgccgac tgaacaatgg
    15421 cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    15481 gactcattcc ttgtaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    15541 acgaaacacc cttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    15601 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa
    15661 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    15721 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg
    15781 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    15841 gttactgttc gcatcccact gcacaatgac acagtcaccc tgtccgacaa tttccagccc
    15901 ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg caacggtaaa
    15961 tacccgcagc cagatttcat cttccagcgc cagcttacgg gtgctcgtta aactttcacg
    16021 ctgtaacgca ggcagaaagc gtgctgcccc cagcaacaat acgctactga tcgccatagc
    16081 aatcaacact tccagcagag aaaaacctag ctcttttaca ggcatccttc tgtttctcct
    16141 tgctgacaaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    16201 tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    16261 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    16321 ccatggcagg tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg
    16381 ataacactga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    16441 tagtcccgcg cctggctggc ggtttgccat agccgttgcg actgctgcca gtattgccag
    16501 ccatagagtc cacttgcgct tagcatgacc agaatcagca tcgcgaccag cgtttcaatc
    16561 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    16621 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggaggttg
    16681 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaaac cgacgcacac
    16741 tggcgtcggc tctggcagga tgtttcgtaa ttagatagcc accggcgctt taatgcccgg
    16801 atgtggatcg tatccttcaa tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg
    16861 tttacgtttg ataatcaact tcggcagcgg acgcggttcg cggcttaatt gcagatgagt
    16921 ttgatccata tgattgctgt acaggtgcgt gtcgccaccg gtccagacaa aatcacccac
    16981 ttccagatcg cactgctgcg ccatcatatg caccagtaac gcgtagctgg caatgttgaa
    17041 cggcaggccg aggaagacgt cacaggagcg ctgataaagc tggcaagaga gtttgccgtc
    17101 tgccacatag aactggaaga atgcatggca cggtgccagc gccattttat ccagttcgcc
    17161 tacgttccac gctgaaacaa taatgcggcg cgaatccggg tcgtttttca gctggttcag
    17221 taccgtagtg atctggtcaa tatgacgacc atctggcgtt ggccaggcgc gccactgttt
    17281 accatacact ggcccgaggt cgccgttttc atcggcccat tcgtcccaga tggtgacatt
    17341 gttttcgtgt agataagcaa tgttagtgtc gccctgcaga aaccacagca gttcatggat
    17401 gatggaacgc aggtggcaac gtttagttgt caccagcggg aatccatcct gcaggttaaa
    17461 acgcatctga tgaccaaaaa tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt
    17521 gccttcgtcg agcacttttt gcatcagttc taaatactgt ttcatggttc ctcaggaaac
    17581 gtgttgctgt gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat
    17641 cgggatggaa agaatttgcc ccatgctgat gtactgcacc caggcaccgg taaactgcgc
    17701 gtcgggctgg cggaaaaact caacaatgat gcgaaacgcg ccgtagccaa tcaggaacaa
    17761 gcctgagact gctcccattg ggcgcggttt acgaatatac aggttgagga taataaacag
    17821 caccacacct tccagcagca gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta
    17881 agtgtcgaaa atggattgcc actgcgggtt ggtttgcagc agcaaaatat cttctgtacg
    17941 ggagccaggg aacagcatgg caaacgggaa gttcgggtca acgcggcccc acaattcacc
    18001 gttaataaaa ttgcccagac gcccggcgcc aagaccaaac ggaatgagtg gtgcgataaa
    18061 atcagagacc tggaagaagg aacgtttagt acggcgggcg aagataatca tcaccacgat
    18121 aacgccaatc aggccaccgt ggaaagacat gccgccgtcc cagacacgga acagatacag
    18181 cggatcggcc ataaactgcg ggaaattgta gaacagaaca taaccaatac gtccaccgag
    18241 gaagacgccg aggaagcccg catagagtaa gttttcaact tcatttttgg tccagccgct
    18301 gcccggacga ttcgcccgac gtgttgccag ccacattgca aaaatgaaac ccaccagata
    18361 catcaggccg taccagtgaa gcgctacggg tcctattgag aaaatgaccg gatcaaactc
    18421 cggaaaatgc agatagctac tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa
    18481 gagaacagcg attgaaatgc gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag
    18541 cacaagggga cctggctggt tgccggatac cgttaaaaga tatgtatatg atccgcgcta
    18601 taaccctccg cgaatcaacc cgcccatgcc gcgacgctcc ataaaggctg caacctgatg
    18661 gcgaacttcg gtcgccagtt gcgcttccag actacgctgc gcaagatttt ctgcttcggc
    18721 ataatcaatg cgccgcagca ggtattttac gcgcgctaca gaacgtccgt tcatagacag
    18781 atggcgatac ccaagcccaa tgaggattgc cacgcacatg ggatcgcccg ccatttcacc
    18841 gcacaaacgg agatcgatac catgtatttc cgcttcccgg gcgatcatcg ccagagctcg
    18901 taacattgca ggatgaagac tgtcataaat gttcgccacc cgggtattgt tgcgatcaac
    18961 ggccaggatg tattgagtca gatcgttggt gccaacagag atgaaatcga cccgctttgc
    19021 cagatgcggc agcataaata ccattgacgg cacttccagc atgatgccga tacgtggttt
    19081 gggaatttcg taaccgatca tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg
    19141 tgcttcgtca acttcatcga ggctggtgac catcggcaac agaatattca ggttgcccgt
    19201 agcggcatta gcacgcagca tcgcccgcac ctggatcaag aagatctccg gctgatcgag
    19261 cgtaatgcga atcccacgcc aacccaggca tggattctct tcgctgatcg gcatgtaagg
    19321 cagctgctta tctgctccga catccagcgt acgcaaggtg acgggtttat cattgaacat
    19381 ttgcagcatc ccctgatact gcgccacctg ttcttcttcc gacggaaaac cactttgcag
    19441 catgaatggg atttcagtgc gataaagtcc tatgccatca atacggctgc ccagtttttc
    19501 ttcatgttcc gggcttaaac cggcattgag catgactttt atacgctcgc cactttttaa
    19561 ctgcgcgggt aaattgacgt catcttccgc cagacggcta agctcaatct cttcactaat
    19621 tagccgctga tattcttgca gcagtaccgg ctccggatcg accagcaatt caccgcgata
    19681 accatcgaca atcagcgtcc gacgatgcag caccgaaggc tgaatatccg cgcccatcac
    19741 ggtagggatc cccagcgcac gtaccatgat cgcagcatgg gagttggctg cgccatcgcg
    19801 cacgacaaca ccgactaagc gatcctgggg cagctcagca agcgttgtcg ctgacagttc
    19861 atctgccacc agaatgaaac gttccggcca ggcgttcggc ccttgattag cgtcatcaag
    19921 atgaaacagc aatcgctgcc ccagcgcacg taaatcgcca gcccgctctt tgagatagtt
    19981 atcgcttagc gcggcaaact gttcggcaaa tttttcaatg accgttttta ccgcccactc
    20041 tgccaccgag cctttatcaa cctcggcaaa caattcgcgc cgcagccggg tgtccgaaag
    20101 caggtgcgag taaagatcga aaatagccgc cgtttctttt tgtgcaccgg cggcaaagcg
    20161 tttgctgtag cggcgaaact cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg
    20221 ttccagagcc ggatccagcg ttgatgcctg atacacctgt tccattaaag gtaacgtggc
    20281 atcctgccag ccttcggcaa tcgccacacc aggcgctgcc ggtaatgcac ggattcgcgt
    20341 ctggcgatat tgtccaaaca aggcagtcaa ctgcgactga gaaagaatag ctgccatctg
    20401 agtggcaagc gtcaccagga aggattcttc actttcgtca tactggcgca actctcgttg
    20461 ctgtaccacc agtacaccaa gcaactggcg acgttgaata attggtacgc ctaaaaacgc
    20521 gcggaaacgt tcttctttta cggaggggat gtatttgaag ctggggtgct tttgcgcatc
    20581 tgcaaggttt atcggttccg ccagcctgcc aaccaggccg acgatccctt catcaaacgc
    20641 gagcgttaca gtgcgaccgc gtggtttttt cagcccccgg gtcgccatca ggtagtaaca
    20701 acgtcgatca tgatcggcaa ggtaaaccga acagacctcg gtatccatcg caagacagat
    20761 gtcggtaacc agaatattta acgcctcatt caggcgtggt gcgctggcta ccttttcgac
    20821 tatttcgcgc aggcgagtga gcataattgg cgtgacttaa cctcttttac gtcgataagc
    20881 agatgcgttt tgtggttttg gcgtattttc ctgcagtgac atcaccacac tcgcgaactc
    20941 tttcattacc ctacggtaga catcacgttt aaatgacacc acctgtctga ccggatacca
    21001 gtaacttacc catcgccagc cgtcaaactc tggtgtactg ctggtttgca tattgatttc
    21061 tgcatcgccg ctcaccagct gcaagagaaa ccatttttgt ttttggccga tacaaaccgg
    21121 cttcgtgtcc caacgcacca aacgtttcgg taatttgtag cgcaaccagt tacgcgttga
    21181 agcaaggatt cgaacgtctt tacggcttaa tcctacttct tcaaacaatt cacggtacat
    21241 cgcctgctct gcggattctc cggggttgat tccgccttgc ggaaattgcc aggagtgctg
    21301 accaaatcgc cgggcccaca ttacctgccc ctggcgatta caaatcacga tacctacgtt
    21361 tgggcggtag ccatcgtcat caatcaccgg actacctcaa aataaagctt tatatacgaa
    21421 tgattgtttc atactccagg aagacggtaa accactctct gcagggcatt acacactaat
    21481 aacaattgaa taactcacag ttatgtgcag agttataaac agaggaaggg gtggatagcc
    21541 gtttttattc accttttctg tggatagagt
//
LOCUS       DARNVM010000073        21504 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.073, whole genome shotgun sequence.
ACCESSION   DARNVM010000073 DARNVM010000000
VERSION     DARNVM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.073"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..279
                     /locus_tag="SI621_002505"
     CDS             <1..279
                     /locus_tag="SI621_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4065210.1"
                     /translation="YICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRV
                     GYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(322..1473)
                     /locus_tag="SI621_002506"
     CDS             complement(322..1473)
                     /locus_tag="SI621_002506"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEI4065211.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1487..3082)
                     /locus_tag="SI621_002507"
     CDS             complement(1487..3082)
                     /locus_tag="SI621_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HEI4065212.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNQEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3227..3985)
                     /gene="aroD"
                     /locus_tag="SI621_002508"
     CDS             complement(3227..3985)
                     /gene="aroD"
                     /locus_tag="SI621_002508"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEI4065213.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVDSVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4016..4882)
                     /gene="ydiB"
                     /locus_tag="SI621_002509"
     CDS             complement(4016..4882)
                     /gene="ydiB"
                     /locus_tag="SI621_002509"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HEI4065214.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4894..6159)
                     /locus_tag="SI621_002510"
     CDS             complement(4894..6159)
                     /locus_tag="SI621_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065215.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6386..7600)
                     /locus_tag="SI621_002511"
     CDS             complement(6386..7600)
                     /locus_tag="SI621_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065216.1"
                     /translation="MKNPYFPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRATT
                     LLMLYTFISFTALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(7699..8055)
                     /locus_tag="SI621_002512"
     CDS             complement(7699..8055)
                     /locus_tag="SI621_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HEI4065217.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8191..8298)
                     /gene="rprA"
                     /locus_tag="SI621_002513"
     ncRNA           complement(8191..8298)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="SI621_002513"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8484..9596)
                     /gene="ydiK"
                     /locus_tag="SI621_002514"
     CDS             complement(8484..9596)
                     /gene="ydiK"
                     /locus_tag="SI621_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEI4065218.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEVLEELGEIEKPNK"
     gene            9985..13041
                     /locus_tag="SI621_002515"
     CDS             9985..13041
                     /locus_tag="SI621_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEI4065219.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTAIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCRDL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDVRSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVLADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13038..13448
                     /gene="menI"
                     /locus_tag="SI621_002516"
     CDS             13038..13448
                     /gene="menI"
                     /locus_tag="SI621_002516"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HEI4065220.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13548..13736
                     /locus_tag="SI621_002517"
     CDS             13548..13736
                     /locus_tag="SI621_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HEI4065221.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14285..14653
                     /gene="sufA"
                     /locus_tag="SI621_002518"
     CDS             14285..14653
                     /gene="sufA"
                     /locus_tag="SI621_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HEI4065222.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14662..16149
                     /gene="sufB"
                     /locus_tag="SI621_002519"
     CDS             14662..16149
                     /gene="sufB"
                     /locus_tag="SI621_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEI4065223.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGVVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16159..16905
                     /gene="sufC"
                     /locus_tag="SI621_002520"
     CDS             16159..16905
                     /gene="sufC"
                     /locus_tag="SI621_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEI4065224.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16880..18151
                     /gene="sufD"
                     /locus_tag="SI621_002521"
     CDS             16880..18151
                     /gene="sufD"
                     /locus_tag="SI621_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEI4065225.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSYLLGGAVLRHNTSSQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18148..19368
                     /gene="sufS"
                     /locus_tag="SI621_002522"
     CDS             18148..19368
                     /gene="sufS"
                     /locus_tag="SI621_002522"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HEI4065226.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGTKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19381..19797
                     /gene="sufE"
                     /locus_tag="SI621_002523"
     CDS             19381..19797
                     /gene="sufE"
                     /locus_tag="SI621_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HEI4065227.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSS
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19946..20950
                     /gene="ldtE"
                     /locus_tag="SI621_002524"
     CDS             19946..20950
                     /gene="ldtE"
                     /locus_tag="SI621_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HEI4065228.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21014..21250)
                     /gene="lpp"
                     /locus_tag="SI621_002525"
     CDS             complement(21014..21250)
                     /gene="lpp"
                     /locus_tag="SI621_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HEI4065229.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5584 a         5222 c         5453 g         5245 t
ORIGIN      
        1 tatatttgcc atgaatttac caaagagtat cgtatttccc ctattaacta tgttattcaa
       61 cggcgtatga cggaagcgaa atggtcactc actaatactg aattatcaca ggcagagatc
      121 tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt gcgccatgtc
      181 ggctgttcgc ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga acaagaaatc
      241 ttatctgaat ttcctcaacc ggtaagtctt gccggataaa tctggcggcg cagtttgtta
      301 agaagctgcg ccgcctgcag attatttgtt ctgataatct ttcaggatct gccgacccgc
      361 tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga catcacgcca
      421 gaagcgagag acgcgcgcct catcggtata gcccagaccg cccatgattt gaatcgcatc
      481 atcaataact tccattgcgg tacgtgcgca gtacagtttt gccagcgccg cgctggtgcg
      541 cagtgactga tgctgatcgg cttgccatgc cactttcagc accatgttgc gcatgttgtc
      601 gatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac caatgggctt
      661 accaaaagcg atacgttggt tggcatagcg ggcggcatct tcaaaggcac actcggcaaa
      721 gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca tcacattgag
      781 gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacgt tgtcgagata
      841 gacttcgcag gtgctgagca tatgccagcc gattttatgc agcgggttaa tcttaatgcc
      901 gggcttactg gagtcgaccc accacagggt gaaggctttt ttgggatctt tcggttctgg
      961 atcgcgcgcc agcaccagca tatacgggta ctctttcgcg ccggtaataa aggttttctg
     1021 gccgttgatg taaaccttgc cgtttttacg cgtgtaagtg gtggtggcgc tgttgttatc
     1081 tgagcctgcg cctggttccg tcaatgccag ggcataggcg ggatcacccg tttccagggt
     1141 gctctctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca
     1201 ttgaccgttg gtgatcaaaa atgccggggc accgcatttt gacacttcca tcagcgccag
     1261 catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc caagcatgga
     1321 aataccgtta tccgccagtg cccgcataaa ttcacgcggg tatgtcccgt tttgatcgca
     1381 ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag
     1441 cagttcttgt tcttcagtta aagaaaaatc catcgtgata ctcctgttaa tgagccgctt
     1501 caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt
     1561 ctggcgaaat cactggggtg aaatccattt tgtcgagaat atctttttgt aaatcgacgc
     1621 caggggcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaatact gcgcgctcag
     1681 tgatataacg aacatccagc cctcgctcga gagcgatttt tccgctgaaa gtaatttccg
     1741 gtagttcccg aataaatttc ttcacccgtc cttcctggac gatatttaat ttgccgtcgg
     1801 taatttctgt ttttaaactg cccgcagtta atgtgccgca gaaaacgatt ttcttcgaag
     1861 tggcactgat atcaataaat ccaccggtgc ccatgatttt accattgaat ttatgcacgc
     1921 cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccgccac
     1981 cgtgataaaa atcaaactgg gacgtcatat ccagaatggc acgggtattc acgttcgcgc
     2041 caaaggcgat cccctgtgaa gtaattccgc caatcggacc tgtttctacc gtcagaataa
     2101 agtcatcagc acaaccttct tctcgggcga ccaggccaat gccgtcagca ataccgacgc
     2161 cgacattccc caccgcgcct ttacgcattt cgaataatgc gcgccgtgca actaatttac
     2221 gttggtttag gggcaggcta agtttggtgc tgtcatcaag ggtgaagtca ccagaaataa
     2281 agcggttaac cggcgcaccg ccatacagtt gggtttgatc cggatcgacc accacaatat
     2341 ccaccagata acccggaata cggacagatt taggatgcag cgtggctttc ttaaccattt
     2401 tctgcacctg catcatcaca ataccgccgt tattgtgcac cgcctgggca ataaccaatg
     2461 cgtcgagata catcacctca tcttcaaaag tggcgtagcc ttcgctgtcg caggtggtag
     2521 cgcgaatgaa ggcgatatct ggcgcaatcg ctttgtaata gagatattct tggttatcaa
     2581 actcgaccag tttaatcagg tcttctttag tgacttcatt cagtttgccg ccttgctggc
     2641 gtggatcaac aaatgtcccg atgccaatat cactaataat accaggctgg tgggctgcgg
     2701 cggcgcgtaa ggtttgtgta agtacacctt gtggatagtt ataagcaata attttatttt
     2761 gttctgcgag atcagaaata cgcggcgatt gtccccagtg accgcataat gcccatttca
     2821 ccagaccttc ttgtgccagc ggactaatac cacggtcggc gcgatcgcca agccctgttg
     2881 gactaataat cgataaatta cgtggtgttt gagtctgttt atatttatca gcaagagcag
     2941 taattaacgt ggtggcttcc agaataccgc cgccagcgcc taacacacaa agtgttgctt
     3001 cgtcgggaat ataattcacc gcttcctgtg ccgacaggac cggcactcgt ccattaatac
     3061 gaggtggttt tacaggtttc attgttaatt cctgattcag atgtgatttt ccctacgcca
     3121 gatggagaat tagcgcacag agactcacga aatattttta ttaaatacgg ttaaatatat
     3181 ttttttagtt cggcggggga ggatgttccc gccgaaatat tattgcttat gcctgatgta
     3241 aaatagttaa tactgtgcgc aaatcattta ccgagatttg ccctggtgca gaggcctttt
     3301 ttaccgcacc aaaagttgcc gccgagccaa atacttcacc agccagacga gaaattacgc
     3361 cagtttttgc cattgacatc gtgatgattg gacgatcggc atactgctcc tgcatctcca
     3421 gggtcgcggc aagcaacgtc agcacatcgc tggtactttg cggcatcagc gcaatcttag
     3481 gaatatcggc gtcgaaggac tgcattttgc gcagacgggc aatgatttct tcggcttccg
     3541 gcgttttatg gaagtcatgg ttggacatga caactttcac atcatgcgcg tgggcgtagg
     3601 cgacggtttc tttaacctga tcatcgccgg taaataactc cagatcgatc atatcaacca
     3661 ggccgctgtc gatggctgca cgattgagtg caatataagc ctcggtggaa atcgcctgct
     3721 cgccgccttc tttggcactg cggaaggtaa acagcagcgg tttttctggc atggtttcac
     3781 ggagaatttt tgccgccgcc atgacagaat ccacattgga gaggtcggca tagtggtcaa
     3841 cacgccattc cagaatatca aagtccgctt cacgataggc gagagcttca gatttcacgc
     3901 tggcgatatc tttcgccatc agcgagacga tgattttagg tgcgcccgta ccaatgacga
     3961 gatcttttac agttacggtt ttcatttttt accctttctg cacgcggtca gcctgtcagg
     4021 caccgaaccc catgacctgt ttaacatatt ccagagggaa atctttgcca gtccataacg
     4081 tgaactgttc agccccttgc cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag
     4141 cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt atacacgcat tcagtgacca
     4201 gaagtcccgg atgtaacaga ctgatatcat taaccaatga ttcattctca aggggtttca
     4261 tacccacttt tgtgccattg gttaaaatgt cggcggaagc cagggcttca gcaaaggctt
     4321 gctgatcggc gagatcggta accgtgacga cacaatcggt gttttcgtta acccgctgcg
     4381 cgaaggcgag ggctttatcg aagaactcat cccgacggtt aaagagttta atttctttta
     4441 aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc acctccggcc cctaacagca
     4501 ccatcgtttt gcctttgata tcaaaaccgc tctctttaat ggcgcgaata tggcccgtgc
     4561 cgtcggtgtt atagccacgc agatagccat catcattaac gatggtgttg atggcaccca
     4621 ccagtttggc cgctggcgtt aattcatcaa catattcaca cgccagttgt ttgttcggca
     4681 ttgatacacc agttccgcgc attttgaggg cttttaatcc ttcaattgct ccgggaaaac
     4741 tatcgttatc cacttcaaaa gccatatagg taaatggcaa tcccgctttt tctaaggctt
     4801 tattctgcat ttcaggcgat aaactgtggc ggatgggata tgccatcagc ccaatcaatt
     4861 cgtattttgc ggtaacatcc ataattaact cctttaacct ctatgcttaa ttgtgtttaa
     4921 ccgggtgcta aatttacgct cgccaaaccg cacatcattt tcaggaataa tgaaaacgcg
     4981 gtaatagcgg ataaagacaa taattgcggt aatcagggcc agcagcgcga aagtaaaatc
     5041 gagagcaatg atatattgca ggccgatgtt cgacagataa ccggtaatca gcggaataac
     5101 aaaattagcc agtccaccca tcatcatata tatactggtg actttggctt tgctttttgg
     5161 gaaaaactct gacatgaccg aaacgccgag ctgtaaaatg ccgccagctg ctgagaaacc
     5221 gataacaaag gctccggcat tacacaccaa tggagaaggg tacaggtaga taatggcagc
     5281 tgttattgtt gccagtgcag agttaaatac attagcccag atgggccgga ccattttttt
     5341 cagcagtgcg gcaaaaataa agacacagac caacgagccc atactgtaat aagagatggt
     5401 ttttaatgcc tcagcttctg acataccagc aaaagccatc gcatatttgg gcatccacac
     5461 cacaatcaca taaaaggtcg agaatgcggc tacaccgaac agtactgatg aaacaccttc
     5521 cagccagact aacggtttgc tgttcatttg cggtaattca ttagctacgc tggcgtccac
     5581 caactggctg gggaatttgc ttttcaacag catcagtgtg atcagtacaa acagaatacc
     5641 cggaataatc agcccatagc cgtaccagat gttattgagc aacatatagc tcaccagcat
     5701 tgggtagaac atttgcccga atgacaccat cgctttaacc agtatgaccg ccgaaccaga
     5761 ggctttcgga aagcattcca tgagcgcggg gtagccaccc gtatccagcg ctgagttagc
     5821 gatacctacg cacactgcca gacagtaggc gagagttaaa ttcgggcaag cgggaatacc
     5881 aaagaagaat agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt
     5941 atcggagatc acaccgaaga ataaaatact gatcaatcgc cccaaaccga taccggaaat
     6001 taaataggca atgcccgcgt tgtcagtgga aaacttttct gccagagatg acatattttg
     6061 ggcaagcgta ataacactaa tgccgtgcag gaaatagctg aagtaaatac aaagaacagc
     6121 caggataaat ggcgtgctga aagccttatt ttgagacatt ttcacagctc cttgctgaaa
     6181 gataatttcg ttaccgtacg tattgttctc cgacggttca cataattact atactgaaga
     6241 tgattttccc tgggtacatg gcataaagtt tatgtttcca ggaatattgc atgtcatagt
     6301 ttgtttttta tcgcgatttt cgttagttgg cgcagaaaga gttatttatc cgtaagatct
     6361 tgcatgacct gaaaagaata ttgcattacc cacccggagc gacattttcc tttagcgagt
     6421 gatgccgcgt ttctttgcgg ctgcgtaagc cgataaacag tgccagtaaa aagccgatgg
     6481 cagcgatggc ggtatcgaac cacataatat cggcaatact tctttgggac agatgagccg
     6541 taatcaacgg aatagtaaag gtcgcaatac tgcccgcact gtaatagatc cctgtagctt
     6601 tacctttagc gtaggggaaa cgttcagcca ttaatgtcag gccaatttgc acaacacctc
     6661 cagcagaggt aaaaccaatg acaaaagcaa atattatcac cacataaaat gtgggatgca
     6721 ggcagacggt aaacagagca gtaaatgaga taaaggtgta cagcatgagt aatgttgttg
     6781 ctcgaacggt attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg
     6841 tgtagatact gagtagtttg atcgacatag tgtatgacat gcctgcaaca aattgtccgt
     6901 actgtgccag ccactggcta accagataaa acgttgccat tgagatatag ccatataagg
     6961 tataactggc taaatcgatt attgagcagc gatgttccgt agagctggtg gttttcttta
     7021 tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca acggtataaa aacagagcgt
     7081 taataaacat aatgcctgcg gcaatcataa aggaccaacc gaaccacagt tcagcccaca
     7141 ctaacaggct aatgattagc ggtaataaaa attgcccgct ggaaacaaat gctttaatta
     7201 aaatattggc tgtacccggt gagcgtggaa aagcttccat caaactggga taggtgcctg
     7261 catcgagaaa actgtttgcc attcccgcca gaaagccaaa aacataagcg ataatgatgt
     7321 tattggtgtg caggatgcca aaaaagaagg ccatatagca acacatcccg agcatgataa
     7381 aagggcggcg accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac
     7441 caatacccag cgatgagata actattgaga cgcctgcggc attagtctgc caaagcgtct
     7501 ccagcgaggc catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa
     7561 aatacaaccc cagtgcggta gggaaatagg gatttttcat aggttctact tagcaaaggt
     7621 gctttaaaaa gtgaagcgat gttgtgtcag catccgttca ttattgttgt aacaacgaca
     7681 attcctttct ttggaaaatt aacaaaggcg ctccagatcg tgtgcatata attcagcggc
     7741 taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt gttgaaacgc
     7801 ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta ttttctctat
     7861 gatggcatga agatgttttt ttctttgctg acgcatagcc aacagttgct cgactacacg
     7921 atcaggaatg gcgtatttgc cattttccca ctgacgccag ctttcactgt caccggtctg
     7981 ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga gtgcctggag
     8041 ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat aaataacatt
     8101 atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta ttggaaagcg
     8161 tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg ggagatgggc
     8221 aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt ataaatcaat
     8281 atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt cgatcagatt
     8341 cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactaccagc tgaatggcgg
     8401 taagcaggaa aatatgggga agagcaagca aatatcaatt agcaggggtt aacaatcagg
     8461 cggcagagcc gcctgatgag aaattactta ttcggtttct cgatttcgcc aagttcttcg
     8521 agaacttctt ccggttggtc agtcggcggt gggacttctt ccacccacgc ggcaaaaaga
     8581 cgccaggaaa cggctaacag aaccggacca ataaacaggc cgatcatccc gaaagcaatc
     8641 aaaccaccaa taacgccaga gagaatcagg atcagcggta aatcggcacc catgcgaatt
     8701 aacattgggc ggatgacgtt atccagcgtg ccaaccacac cgctccacac taacaatacc
     8761 gttccccagg tggtatcgcc agtccagtag agccagataa tcgccggaat cagtaccggc
     8821 aacggtccaa gctggacaag gcaggagagg atcattaaca ccgtgagcaa agttgcataa
     8881 ggtacgccgg atacggcgag gccgataccg ccaagcactg cctgtactaa cgctgtcacc
     8941 acaacaccca gcgccaccgc gcggatagct tgtgccgcca gcagcacggc ggcatctcca
     9001 cgaacgcctg ccagacgggt tgcaaaatgg cgaatgcctt gcgccacttg ttcaccgcgc
     9061 cagtacagca gggcgctgaa gagaagcatc agcgcacaat gcaccataaa gcgcccgata
     9121 tgcgccgcct gcccaacgaa ccaggtggtg gtggtgccaa tataaggacg gacttttgcc
     9181 atgatcgccg tcccccccat atccagcaag ttgtgccagc ctgcatacag cttcgcgcca
     9241 atcaccggaa tggtattaag ccacgccaga tcgggtaacg tcatgtcacc gctggaaatg
     9301 gctttaatta gcgggccgct gccgtcgacg atactgttaa ccagcaaggc aatagggatg
     9361 ataaacacca tcactaataa cagcgtcatc accagaacgg cgagagagcg gcgaccaaac
     9421 atgatctttt gcaaacgtaa caataccggc caggtggcga taaccaccgt accagcccat
     9481 gcaaagccga gaataaaggg ttgtacaatc cacagacatg ccacaatcat gatggctaaa
     9541 aaaagcaccg aaagcagaat ttgtgcgaca tccctgggct gacgaacatt taccataact
     9601 acttttcacc ttttttgtgc gtcaaatcgt cgacgcgacg tgaacacgcg aaactcacct
     9661 gcataatgat gagcaatttc agcggttttt aacaggccga ttctgccctt aattctgatg
     9721 ggcgtgttag aaaaaaatgt ggtacaacaa ctcaggcaac acgcaaacga tttactcgtc
     9781 gtatttcaag ttgcatgtgc tgcggctacg ttcgttcacc ccagtcactt actattgtaa
     9841 gctcctggag ttcactcgct tgccgccttc ctgcaactcg aaatccatag agtaaataaa
     9901 catcaccttt cagtgcgcta ttgcagcagc ctgaaagaat agtgtatcga cctacatcac
     9961 agaccgcagg aaagggtcaa tataatgatt ccacagattt cccaggcacc cggcgtcgtt
    10021 caactggtgc ttaatttttt gcaagagctg gagcaacaag gttttaccgg cgatacggcg
    10081 acaagttatg ccgatcgtct gacaatgtcg accgacaaca gtatttacca acttctcccc
    10141 gatgcggtgg tatttccgcg ttcaaccgca gatgtggcgc tgatcgcccg tcttgccgcg
    10201 caggaacgct attcatcgct gatctttacc ccccgcggcg gcggcaccgg cactaacggt
    10261 caggcgctca accaggggat tattgttgat atgtcccgcc atatgaaccg catcatcgaa
    10321 attaaccctg aagagggctg ggtgcgcgtt gaggccgggg tgataaaaga tcaactcaat
    10381 cagtacctga aaccgttcgg ctactttttt gcgccggaac tttcgaccag caaccgggca
    10441 acgctcggtg ggatgatcaa taccgatgca tccggtcagg gatcgctggt ctatggcaaa
    10501 acgtcagatc acgtacttgg cgtacgcgcg gtgttgttgg ggggcgatat tctcgatacg
    10561 caacctttac ccgtcgaact ggcagaaacg ctgggtaaaa ccaataccgc aatcgggcga
    10621 atttataaca cggtttatca acgttgccgt cagcaacgcc agttaatcat cgacaacttc
    10681 cccaaactta accgctttct taccggttac gatctgcgtc atgtctttaa cgatgagatg
    10741 accgagttcg acctgacgcg cattctgacg ggttcagaag ggacgctggc ctttattacc
    10801 gaagcgcggc tggatattac gcgcttgcct aaagtgcggc gtctggtgaa cgtcaaatat
    10861 gactcttttg actccgcgct gcgtaacgcg ccgtttatgg ttgaggcgcg ggcgctttcg
    10921 gtagagacgg tggactcaaa agtgctgaat ctggcgcggg aagatattgt ctggcattcc
    10981 gtcagcgagt tgattaccga tgtgcctgac aaagagatgc tcgggctgaa cattgtggaa
    11041 tttgctggtg atgatgaggc gctgattgac gagcgagtta atgcactctg tgtgcggctt
    11101 gatgagctga ttgccagcca acaggcgggg gtgattggct ggcaggtgtg ccgcgatctg
    11161 gcgggtgttg aacgtatcta tgcgatgcgc aaaaaagctg tcggcctgct cggtaatgcc
    11221 aaaggagcag ccaagccaat tccgtttgct gaggatacct gcgtaccgcc ggaacatctg
    11281 gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc acggcttaag ctacggtatg
    11341 ttcggtcacg tcgacgcagg tgtcttgcac gtccgtcctg cgctggatat gtgcgatccg
    11401 caacaagaga ttttgatgaa gcaaatctct gatgacgtag tggcgcttac cgcgaaatac
    11461 ggtggtttgt tgtggggcga gcacggcaaa ggttttcgcg ctgaatacag cccggcgttt
    11521 ttcggtgagg aactttttgc agaactgcgc aaagtgaaag cggcatttga cccgcataac
    11581 cgactcaacc cagggaagat ttgcccgcca gaaggtctcg atgcgccgat gatgaaagtg
    11641 gacgcggtga agcgcggtac attcgatcgg cagatcccca ttgcggtacg ccagcagtgg
    11701 cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca actttgatgt ccgtagtccg
    11761 atgtgtccgt cgatgaagat cacccagaac cggattcatt caccgaaagg gcgcgcaacg
    11821 ctggtgcgtg aatggctgcg cttgttggcg gatcgcggcg ttgacccact caaactggaa
    11881 caagaactgc ctgaatccgg cgtcagtttg cggacgttaa ttgcccgcac gcgcaatagc
    11941 tggcatgcga ataaaggcga atacgacttc tcacacgaag tcaaagaggc aatgtcgggc
    12001 tgtctggcct gtaaagcgtg ttcgacccag tgccccatca aaattgatgt gccggagttt
    12061 cgctctcgtt ttctgcagct ctatcacacc cgttatttac gcccgcttcg cgaccacctc
    12121 gttgctacgg tcgagagcta cgcaccgctg atggcacgcg cgccgaaaac ctttaacttc
    12181 ttcattaacc agccgctggt gcgcaaactc tcggaaaaac atatcggcat ggttgatttg
    12241 ccactgctgt cagtcccctc gctacaacaa caaatggtgg ggcatcgctc ggcgaatatg
    12301 acgctggaac agcttgaagc tctcaatgca gagcagaaag cgcgcacggt gttggtggtg
    12361 caggacccct ttaccagcta ttacgatgca caagtgctgg cggattttgt ccgtctggtt
    12421 gaaaaattag gattccagcc tgtgttactg ccattttcgc caaatggcaa agcccagcat
    12481 atcaaaggct ttcttaatcg ttttgcgaag acggcgaaaa agacggcgga tttcctcaac
    12541 cgtatggcga agctggggat gccaatggtg ggcgtcgatc cggcgctggt actttgttat
    12601 cgcgatgaat ataaactggc gctgggcgag gaacgtggcg agtttaacgt cttactggcg
    12661 aatgaatggc tggcaagcgc acttgagtca cagccagtgg ctacagtcag cggtgaatca
    12721 tggtatttct ttggtcactg taccgaagtt accgctttgc cgggcgcgcc agcacaatgg
    12781 gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg tcagcgtggg ttgctgcggc
    12841 atggcaggga cttacggaca tgaagcgaaa aaccataaaa attcgctcgg gatctatgag
    12901 ttatcctggc atcaggcgat gcagcgactg ccgcgtaatc gctgtctggc gaccggatat
    12961 tcctgccgta gccaggtaaa acgggtcgaa ggcacggggg tacgccatcc tgtgcaggct
    13021 ttactggaga ttattaaatg atatggaaac gaaaaatcac cctggaagca ctgaatgcca
    13081 tgggtgaagg aaacatggtg gggttgctgg atattcgctt tgaacatatt ggtgatgaca
    13141 cccttgaagc gacaatgcca gtagactcgc ggacaaagca gcctttcggg ttgctgcatg
    13201 gaggggcatc cgtggtactg gccgaaagta tcggttccgt tgccggttat ttatgtaccg
    13261 aaggtgagca aaaggtggtt ggtctggaaa tcaatgctaa ccacgtccgc tcggcacgag
    13321 aagggcgggt gcgcggcgta tgcaaaccgt tgcatctcgg ttcgcgtcac caggtctggc
    13381 agattgaaat cttcgatgag aaagggcgtt tgtgctgttc gtcacgattg acgaccgcca
    13441 ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc ttttggtcat tatctgcaca
    13501 gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga ggatgctatg tctactcaac
    13561 ttgatcccac ccagctggcc attgaatttt tacgccgtga ccaaagcaat ctttcacctg
    13621 cccaatatct caaacgtctg aaacaactag aacttgaatt tgctgacctg cttactctct
    13681 cttctgctga attaaaagaa gaaatctact ttgcctggcg tctgggcgtg cattgatcct
    13741 gacttgatgc cgcaatttcg ccaaatcgac ctggattaac ctccgtccag gttcgatggc
    13801 cttcttaata ctgcttatct acccgcctga tattgttcaa aaataatact aatcgcagtt
    13861 tgtgttaaaa cggcgggtta gctttatgag attattctta tcgccccttc aagagctaag
    13921 ccactgagag tgccggagat aagcgccgga tggggtagaa acccttaagc ctgtgtcgca
    13981 cagacttaag ggttttctta tttctgaacg ctgcactttt ttacctatct cttaaatagt
    14041 cattttcaat acaacttttt ctttttcatc acagccttaa tgctccttag ataataatca
    14101 ttatctaaca atgagatacc taattcttag cgtgcctgtt aacccacaca tcagggtcta
    14161 tgcttattaa aagcaggaaa aaagcatgtt ttacggtaaa gcccctgcgt ttgctgggtt
    14221 gaactgataa tcattatcac taacatgctg ttatacgctg aaagcgatga agtgaggtaa
    14281 atcgatggac atgcattcag gaacctttaa tccacaggat ttcgcctggc agggcttaac
    14341 gctgacaccc gcagccgctg tccacatccg cgagctggtg gcaaagcagc cgggtatggt
    14401 cggcgtgcgc ttaggcgtga agcaaacggg ctgcgcgggc tttggctatg tgctcgacag
    14461 tgttagcgag ccggacaaag acgatctgct gtttgaacac gacggcgcga agctgtttgt
    14521 cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc gattttgttc gtgaaggact
    14581 taatcagata ttcaaatttc acaaccctaa agcccagaat gaatgtggct gtggcgaaag
    14641 ctttggggta taggcggtac tatgtctcgt aatactgaag caactgacga tgtcaaaacc
    14701 tggaccggcg gcccgctgaa ttataaagaa ggattcttca cccagttagc caccgatgag
    14761 ctggcaaagg ggataaacga agaggtggtg cgcgcaattt cggcgaagcg taatgagccg
    14821 gagtggatgc tggagtttcg tctcaacgcc tatcgcgcat ggctggagat ggaagaaccg
    14881 cactggttga aagcgcacta cgacaagctg aattatcagg attacagcta ctactcagca
    14941 ccatcgtgcg gtaattgtga cgacacttgt gcgtctgaac ccggcgtggt gcagcaaact
    15001 ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt ttgagcagtt gggcgttccc
    15061 gtgcgggaag gcaaagaggt ggcggtggac gccattttcg actctgtttc ggttgccacc
    15121 acttatcgcg aaaaactggc ggagcaggga attattttct gttcctttgg cgaggcgatc
    15181 cacgatcacc cggaactggt gcgtaaatat ctcggcaccg tggtgccggg gaatgacaac
    15241 ttctttgccg cacttaatgc ggcggtagcc tctgacggta cgtttattta tgtgcctaaa
    15301 ggcgtgcgct gcccgatgga actttccacc tattttcgta ttaacgcgga aaaaaccggg
    15361 cagtttgagc gcaccattct ggtggccgac gaagacagct acgttagcta cattgaaggc
    15421 tgttccgctc cggtgcgtga cagctatcag ttacacgcgg cggtggtgga agtcatcatc
    15481 cataaaaacg ccgaggtgaa atattccacg gtacaaaact ggttccctgg cgataacaac
    15541 accggcggta ttctcaactt cgtcaccaag cgtgctttgt gcgaaggcga aaacagcaaa
    15601 atgtcatgga cgcaatcaga aaccgggtca gcgattacgt ggaaatatcc cagctgtatt
    15661 ttgcgcggcg ataactccat tggtgagttt tactcagtgg cgctgaccag cggtcatcag
    15721 caagcggata ccggcaccaa gatgatccac atcggtaaaa acactaaatc gaccattatc
    15781 tcgaaaggga tctctgccgg acatagtcag aacagttatc gcggcttagt gaaaatcatg
    15841 ccgacggcaa ccaatgcgcg caatttcact cagtgcgact caatgctgat tggcgctaat
    15901 tgtggggcgc ataccttccc gtatgtcgaa tgtcgcaata acagcgcaca actggagcac
    15961 gaggcaacaa catcacgtat tggtgaagat caactgtttt actgcctgca acgcgggatc
    16021 agcgaagaag acgccatctc gatgattgtt aacggtttct gcaaagacgt gttctcagaa
    16081 ctgccgctgg aatttgccgt tgaagcacaa aaactcctcg ccatcagtct tgaacacagc
    16141 gtcggataag gaataaacat gttaagtatt aaagatttac acgtcagcgt ggaagataaa
    16201 gctatcctgc gtggattaag cctcgacgtt cgtcccggcg aagttcacgc cattatgggg
    16261 ccaaacggtt ctggcaaaag taccttatcg gcaacgcttg ccgggcgaga agattatgag
    16321 gtgacgggcg gcacggttga gttcaaaggc aaagatttgc ttgcgctgtc gccggaagat
    16381 cgtgcaggtg aaggcatctt tatggccttc cagtatccgg tggaaattcc cggcgtcagt
    16441 aaccagtttt tcctgcaaac ggcacttaat gcggtgcgca gctatcgcgg ccaggaaacg
    16501 ctcgaccgct ttgattttca ggatttgatg gaagagaaaa tcgctctcct gaagatgccg
    16561 gaagatttat taacccgttc ggtaaacgtt ggtttttccg gcggcgagaa aaagcgcaac
    16621 gatattttgc aaatggcggt gctggaaccg gagttatgca ttcttgatga gtcggactcc
    16681 gggctggata ttgacgcatt aaaagtggtt gccgatggcg tgaactcgct gcgtgacggc
    16741 aagcgctcat tcatcattgt tacgcactac caacgcattc tcgactacat caagcctgat
    16801 tacgtccatg tgctgtatca gggacgaatt gtgaaatccg gagatttcac gttggtcaaa
    16861 caactggagg agcagggtta tggctggctt accgaacagc agtaacgcgc tgcaacagtg
    16921 gcatcacttg tttgaagctg aagggacaaa acgctcaccg caagcacagc agcatttaca
    16981 acaattgctg cgtaccggac tgccgacacg gaaacatgaa aactggaaat atacgccgct
    17041 ggaagggctg accaatagcc agtttgtcag cattgcggga gagatatccc cacagcagcg
    17101 tgatgcctta gcgttaacgt tagacgccgt gcggctggtg tttgttgatg gacgttacgt
    17161 gcccgcgctg agcgatgcaa ctgaaggcag cggatatgaa gtgagcatta acgacgaccg
    17221 tcaggggtta cccgacgcta ttcaggcgga agtgtttctg catttgacgg aaagcctggc
    17281 acaaagcgtg acgcatatcg ccgtgaagcg cggtcaacga ccggcaaagc cattgctgtt
    17341 aatgcatata acccagggcg tggcaggtga agaggtgaac actgcccatt accgacatca
    17401 tctggatctg gcggaaggtg ccgaagcaac ggtgatcgaa cattttgtca gccttaatga
    17461 tgctcgtcat tttactgggg cacggttcac tatcaatgtc gcagcgaacg cccacttgca
    17521 gcatatcaag ctggcgtttg aaaacccgct cagtcaccac tttgcccata acgatttgtt
    17581 gctggcagac gatgccaccg catttagcca cagttacctg ctgggtggcg cagtgttacg
    17641 acacaacacc agttcgcaac tcaatggcga aaacagcacg ctgcggatca atagcctggc
    17701 gatgccggtg aaaaacgagg tgtgtgatac ccgtacctgg ctggaacaca ataaaggttt
    17761 ttgtaacagc cgacagttgc acaaaactat cgtcagcgac aaaggccgcg cggtatttaa
    17821 cggtttgatc aacgtcgcgc agcacgccat caaaacggat ggtcagatga ccaacaataa
    17881 tctgctgatg ggcaaactgg cggaagtgga tacgaaacca cagctggaaa tctatgcaga
    17941 tgatgtgaaa tgcagccacg gcgcgacggt ggggcgtatt gatgacgaac agatgtttta
    18001 tctgcgctcg cgcgggatca atcagcaaga tgcccagcag atgatcatct acgcctttgc
    18061 tgctgaactg acggaagcac tgcgtgatga ggggcttaaa cagcaggtgc tggcccgaat
    18121 cggtcaacga ctgccaggag gtgcaagatg actttttccg tcgacaaagt gcgggccgac
    18181 tttccggtgc tttcgcgtga ggtaaacggt ttgccgctgg cttatctcga cagcgccgcc
    18241 agtgcacaga aaccgagcca ggtgattgac gccgaggccg agttttatcg tcatggctac
    18301 gcggcggtgc atcgcggtat tcatacctta agcgcccagg cgaccgagaa aatggagaac
    18361 gtgcgcaagc gggcatcgct gtttattaat gcccgttcgg cggaagagct ggtgttcgtc
    18421 cgcggcacga cggaagggat caatctggtc gccaatagct ggggtaacag caatgtgcgg
    18481 gcgggcgata acatcatcat cagtcagatg gagcaccacg ctaacattgt tccctggcag
    18541 atgctttgcg cacgcgttgg cgcagagctg cgtgtgatcc cgctcaatcc cgacggtacg
    18601 ttgcaactgg agacgctacc tacgctgttt gatgagaaaa ctcgcctgct ggcaattact
    18661 catgtctcca acgtgctggg aacggaaaat ccgctggcgg aaatgatcac gcttgcgcat
    18721 cagcatggca caaaagtact ggtggatggc gctcaggcgg tgatgcatca tccggtggac
    18781 gttcaggcgc tggattgcga tttttacgtg ttctccgggc ataaactgta tggccccact
    18841 gggattggca ttctttatgt caaagaagcc ttgttgcagg agatgccgcc gtgggaaggg
    18901 ggcggttcta tgatcgccac cgtcagcctg agtgaaggca ctacctggac caaagcacct
    18961 tggcggtttg aagccggtac acccaatacc gggggcatca ttggtcttgg cgcggcgctg
    19021 gagtatgtgt cggcgctggg gcttaataat atagccgagt atgaacagaa tctgatgcac
    19081 tacgcgctat cacagctgga atctgtaccg gatctcactc tctatggtcc ccagaacaga
    19141 cttggcgtta ttgcttttaa tcttggtaaa caccacgcct atgatgttgg cagttttctc
    19201 gataattacg gcattgctgt gcgtaccgga catcactgcg ctatgccatt aatggcctat
    19261 tacaacgttc ctgccatgtg tcgggcgtcg ctggctatgt ataacaccca tgaagaagtg
    19321 gatcgtctgg tgaccggcct gcaacgtatt caccgtctgc tgggataaca gggaggcact
    19381 atggctttat tgccggataa agaaaaattg ctgcgtaatt ttttacgctg cgccaactgg
    19441 gaagagaaat atctctacat tattgagctg ggccagcgtc tgccagaatt acgcgacgaa
    19501 gacagaagct cacaaaatag cattcagggc tgccagagtc aggtgtggat tgtcatgcgc
    19561 cagaatgcgc agggaattat tgaattgcag ggcgacagcg atgcggcgat tgtgaaaggg
    19621 cttattgcgg tcgtctttat tctctacgat cagatgacgc cgcaggatat tgtcaatttc
    19681 gatgtgcgtc cgtggtttga aaaaatggcg ctcactcaac atctcacccc atctcgttca
    19741 caaggtctgg aagcgatgat tcgcgcaatt cgcgccaaag ccgctgcact tagctaaact
    19801 aaaggaacag ctttcatccg gttggctcac attgccggat ggtcaatgtt tacactgacc
    19861 tgcacaggag tgttttggtt tcaggtgaac ataagcgcca tccgacgttt cagcgtgagt
    19921 ctccggcaaa tacaggaggt ttacaatgaa acgcgcgtct ttgcttacac tcacgcttat
    19981 cggcgctttt agcgccatcc aggctgcctg ggcggttgat tatccgctac ctccaaccgg
    20041 aagccgactg gttgggcaaa atcaaacgta tacggtgcaa gaaggggata aaaaccttca
    20101 ggccatcgcc cgacgttttg atactgcggc aatgttgatc cttgaagcca ataacactat
    20161 cgccccggtg ccaaaacctg gtacgacgat aactattcct tcgcaactgt tattacctga
    20221 tgcgccgcgt caggggatta tcgttaacct ggcagagctg cgcctttatt attatccgcc
    20281 gggagaaaat atcgtgcagg tctatccgat aggtattggt ttgcaggggc tggaaacgcc
    20341 agtgatggaa acgcgtgtgg ggcagaaaat ccctaaccca acctggacgc ctacggcggg
    20401 cattcgtcag cgttcgctgg agcgtggcat taaattaccg ccagtcgttc ctgccgggcc
    20461 aaataacccg ctaggacgtt acgcactgcg cctcgcgcat ggtaatggcg aatacctcat
    20521 tcatggcacc agtgcgccgg acagcgtcgg tttgcgcgtc agttcgggct gtattcgcat
    20581 gaatgcgccg gatattaaag ccttattctc cagcgtgcgg acgggaacgc cggtgaaagt
    20641 gatcaacgaa ccggtgaaat attccgtgga accgaacggg atgcgttatg ttgaagtaca
    20701 tcgaccacta tcagcagaag aacagcagaa cgttcagaca atgccataca cactgccagc
    20761 aggctttacg caatttaaag acaataaggg tgtagatcag aaattagtcg ataaagcgtt
    20821 gtatcgtcgg gcagggtatc cggttgcggt gagcagtgga gcaactcccg cagccagcaa
    20881 tgcgccttca gtagagtcag cgcagaatgg tgaaccagag caagggaata tgttacgcgc
    20941 gacgcagtag cggtaaatgg caggcaaaaa aaatggcgca caatgtgcgc catttttcac
    21001 ttcacaggta ctattacttg cggtatttag tagccatgtt gtccagacgc tggttagcac
    21061 gagctgcgtc atctttagca gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca
    21121 gctggtcaac tttagcgttc agagtctgaa cgtcagaaga cagctgatcg attttagcgt
    21181 tgctggagca acctgccagc agagtagaac ccaggattac cgcgcccagt accagtttag
    21241 tagctttcat tattaatacc ctctagattg agttaatctc catgtagcgt tacaagtatt
    21301 acacaaactt ttttatgttg agaatatttt tttgatggga aggcacttat ttttgatcgt
    21361 tcgctcaaag aagcatcgaa cgctgggttt tagataaaaa aattgagaga aaacaggatg
    21421 cttccatcgg attcatctta gataaagtga gattatatag tgaaatccga tttgattttt
    21481 tattgcttct ggttatcgat taaa
//
LOCUS       DARNVM010000074        21374 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.074, whole genome shotgun sequence.
ACCESSION   DARNVM010000074 DARNVM010000000
VERSION     DARNVM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.074"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..535
                     /gene="pphB"
                     /locus_tag="SI621_002526"
     CDS             <1..535
                     /gene="pphB"
                     /locus_tag="SI621_002526"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HEI4065230.1"
                     /translation="ETDLLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDA
                     FETGDGNMWLASGGDWFFELNETEQQEAINLLLKFHRLPHIIEITNDIIKYVIAHADY
                     PGNEYLFGKEIAERELLWPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIY
                     IDTGSPKSGRLSFYKIK"
     gene            complement(586..1353)
                     /locus_tag="SI621_002527"
     CDS             complement(586..1353)
                     /locus_tag="SI621_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4065231.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIACASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASSALAKQDLSLLVAKNE"
     gene            1549..2457
                     /locus_tag="SI621_002528"
     CDS             1549..2457
                     /locus_tag="SI621_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEI4065232.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2454..3716
                     /locus_tag="SI621_002529"
     CDS             2454..3716
                     /locus_tag="SI621_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEI4065233.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3713..4351
                     /locus_tag="SI621_002530"
     CDS             3713..4351
                     /locus_tag="SI621_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEI4065234.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4356..5132
                     /locus_tag="SI621_002531"
     CDS             4356..5132
                     /locus_tag="SI621_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEI4065235.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            5221..6585
                     /locus_tag="SI621_002532"
     CDS             5221..6585
                     /locus_tag="SI621_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HEI4065236.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6624..6860)
                     /locus_tag="SI621_002533"
     CDS             complement(6624..6860)
                     /locus_tag="SI621_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065237.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(6871..8298)
                     /locus_tag="SI621_002534"
     CDS             complement(6871..8298)
                     /locus_tag="SI621_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEI4065238.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     VAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(8298..8891)
                     /locus_tag="SI621_002535"
     CDS             complement(8298..8891)
                     /locus_tag="SI621_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEI4065239.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYNARDVAALADFCHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            9038..9445
                     /locus_tag="SI621_002536"
     CDS             9038..9445
                     /locus_tag="SI621_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEI4065240.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMNT"
     gene            complement(9566..10558)
                     /gene="rpoS"
                     /locus_tag="SI621_002537"
     CDS             complement(9566..10558)
                     /gene="rpoS"
                     /locus_tag="SI621_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEI4065241.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(10621..11760)
                     /gene="nlpD"
                     /locus_tag="SI621_002538"
     CDS             complement(10621..11760)
                     /gene="nlpD"
                     /locus_tag="SI621_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HEI4065242.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(11900..12526)
                     /gene="pcm"
                     /locus_tag="SI621_002539"
     CDS             complement(11900..12526)
                     /gene="pcm"
                     /locus_tag="SI621_002539"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEI4065243.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(12520..13281)
                     /gene="surE"
                     /locus_tag="SI621_002540"
     CDS             complement(12520..13281)
                     /gene="surE"
                     /locus_tag="SI621_002540"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEI4065244.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(13262..14311)
                     /gene="truD"
                     /locus_tag="SI621_002541"
     CDS             complement(13262..14311)
                     /gene="truD"
                     /locus_tag="SI621_002541"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEI4065245.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(14308..14787)
                     /gene="ispF"
                     /locus_tag="SI621_002542"
     CDS             complement(14308..14787)
                     /gene="ispF"
                     /locus_tag="SI621_002542"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEI4065246.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(14787..15497)
                     /gene="ispD"
                     /locus_tag="SI621_002543"
     CDS             complement(14787..15497)
                     /gene="ispD"
                     /locus_tag="SI621_002543"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEI4065247.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKHVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(15516..15827)
                     /gene="ftsB"
                     /locus_tag="SI621_002544"
     CDS             complement(15516..15827)
                     /gene="ftsB"
                     /locus_tag="SI621_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEI4065248.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(16021..16344)
                     /locus_tag="SI621_002545"
     CDS             complement(16021..16344)
                     /locus_tag="SI621_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HEI4065249.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVIGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(16394..16999)
                     /gene="cysC"
                     /locus_tag="SI621_002546"
     CDS             complement(16394..16999)
                     /gene="cysC"
                     /locus_tag="SI621_002546"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEI4065250.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(16999..18426)
                     /gene="cysN"
                     /locus_tag="SI621_002547"
     CDS             complement(16999..18426)
                     /gene="cysN"
                     /locus_tag="SI621_002547"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEI4065251.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVGYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADESLPAV
                     QSASVDVVWMAEQPLSPGQRYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATTAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(18428..19336)
                     /gene="cysD"
                     /locus_tag="SI621_002548"
     CDS             complement(18428..19336)
                     /gene="cysD"
                     /locus_tag="SI621_002548"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEI4065252.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            19588..20625
                     /gene="iap"
                     /locus_tag="SI621_002549"
     CDS             19588..20625
                     /gene="iap"
                     /locus_tag="SI621_002549"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HEI4065253.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGTSWHDVRLDNQQHIDKALPGRIERRCRDVIRIM
                     LPLVKELAKAS"
     repeat_region   20707..21345
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20707..20735
                     /rpt_unit_seq="cggtttatccccgctgacgcggggaacac"
BASE COUNT         4986 a         5751 c         5488 g         5149 t
ORIGIN      
        1 tgaaaccgac ttacttattt ctgtcggcga taatattgat cgtgggccgg agagtcttaa
       61 cgtactacgc ctgttaaacc agccctggtt tacatcggtt aaaggcaacc atgaagcgat
      121 ggcgcttgat gcattcgaaa ctggcgatgg aaatatgtgg cttgccagcg gcggcgactg
      181 gttctttgaa ttaaatgaga cggaacaaca agaagcaata aatctgttgc tgaaatttca
      241 tcgccttcca catattattg aaatcactaa cgacataata aaatatgtca tcgcacatgc
      301 agattatccg gggaatgaat atctctttgg caaagaaata gcggagagag aactactctg
      361 gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa acggcgctga
      421 ttattttata tttggacata tgatgtttga taacattcag acgttcgcaa accagattta
      481 tattgatacc ggatcgccga aaagcgggcg gctgtcattt tataaaataa agtagtctca
      541 tgcttcttct gtgaagcatg agtaacccgg tgttattgca ggccattatt catttttcgc
      601 taccagcaaa gagagatcct gcttcgccag cgcgctactg gcactctccg gcaaaccgtc
      661 gtctgtaata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg ccacctgacc
      721 atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcacaggcaa tcgcccgttt
      781 caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg acgatgcaga
      841 aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt ccccgacaca
      901 ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat tactgttgtc
      961 gagcaagtag tccgcaataa cgaaatcgtt tgtgaccaca gtcagtgact ccatgtgaat
     1021 cagatgctgt gctatcgcta gcgtggtcgt tcccgcatcc agatagatac aacttcccgg
     1081 ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg ccagcgcagt
     1141 ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc ctccggacac
     1201 cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg tcatatgtga
     1261 cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag ctaccatctc
     1321 aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt gtcctgctga
     1381 cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag ttcacataaa
     1441 ataacacaca atgttaattt ttgtgaatca gctcaccata tcgttatctt ccagcgccta
     1501 tattcacaat atcaaacaaa atatcactta aattaacaag gagagcagat gaaaacggga
     1561 tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc agcactgtca
     1621 tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc ctgcgctacg
     1681 ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc cgaaaaactg
     1741 gacgctctgc tggtgctggt ggtcaatgcg gcccaggtta aacaggtgct gtttggtgaa
     1801 acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc cactatcgct
     1861 agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga aatgctggat
     1921 gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt catggcctcc
     1981 ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc cggaaaagtt
     2041 tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca ccagttgtta
     2101 gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg tgcggggatc
     2161 ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg gatgttcgaa
     2221 aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt cgatattttt
     2281 gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc gctgccattg
     2341 gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg gaaagaagac
     2401 gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa atcatgatca
     2461 agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt tttctggtgg
     2521 aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg ccggaagcaa
     2581 tcgacgcact ggtgatcagc ctgaaaacgc gttcctgtcc ggtggttgaa gccacacagc
     2641 aatcgctggc ggcactgagc tggttgcaac agcaaggttg caaacagatc tatttcaaat
     2701 actgctctac tttcgacagt tcggcgaaag gtaatatcgg cccggttacc gatgcattaa
     2761 tggatgctct cgacacgccg tttacggtct tctctccggc cctgccggtc aacggacgta
     2821 cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc gggatgcgcc
     2881 atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa gcgcaatcca
     2941 cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggtgtggat gccgttcgtc
     3001 aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat gcgctgaccg
     3061 aacaccatct ggaaattcag ggagaagcct tgcgcgatac cccactggta acgggcggtt
     3121 ctggtctggc gattggcctt gcccggcagt gggcgcaaga aaacggtaac caggctcgca
     3181 aagcagggca tccgctcgct gggcgcggcg tagtgctctc cggttcatgc tctcaaatga
     3241 ccaaccgcca ggtagcacat taccgtcaaa ttgcaccagc ccgtgaagtt gatgtggcac
     3301 gctgcctctc aattgaaact ctggccgctt atgcacacga actggcagag tgggttctgg
     3361 gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac gcattggcag
     3421 caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaaca ctgttttctc
     3481 aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca ggcggtgaga
     3541 cctccggcgt agtcacacag agcctgggaa taaaagggtt tcatattggc ccaaccattt
     3601 ctcccggcgt gccgtgggta aacgcactgg ataaacctgt ctcactcgcc cttaaatctg
     3661 gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta tcatgagcga
     3721 tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca gttcattctt
     3781 tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac ctgacgggaa
     3841 tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc ggctttccaa
     3901 agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg tgctctttca
     3961 tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc acagcacatg
     4021 gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc gtccgttcac
     4081 accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc gaccgggcga
     4141 taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt ttttactggc
     4201 aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca atatggaaga
     4261 gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc gttatctgac
     4321 cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca gctaatttat
     4381 ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga aaagccgggt
     4441 tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc caaaagcaac
     4501 tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat attaatgcgg
     4561 gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca gacatcgacc
     4621 tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg gcaggcgtcg
     4681 tgcccgctgg cgaagatgct gaacggtaca gggcagtatt tatcgataat ctccgctacg
     4741 ctgccgaccg ctttgcgcca cacggcaagc gaatattagt tgaagcactc agccccggcg
     4801 ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt gaagaagttg
     4861 cacgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta gatggcaacc
     4921 tgacgcattt aatccgcgac tacgccggga aatatgcgca cgtacaaatt gccggactac
     4981 ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc cgcctgttcg
     5041 atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc ctcaccgaag
     5101 aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt acaaagtacc
     5161 catacatccc cccatctacg gatggggtag gttaactata actttcagac agggtttccc
     5221 atgtccacaa ttacattgtt atgcattgcg ttaactggcg taatcatgct gttgctgctg
     5281 gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct gttagtcgca
     5341 cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg gatgggcggt
     5401 gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg tatgatcgaa
     5461 cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg tgacaaacga
     5521 actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt ctttgatgtc
     5581 ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa aatatcgcca
     5641 ctcaaatttg gcctgcctgt cgctggaatc atgctaactg ttcatgtggc ggtaccgccg
     5701 catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg gctaaccatc
     5761 atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc gaaaataatc
     5821 aataagcgcc aatatgcgat gtcggtggaa gtactggaac agatgcaact ggctccggcc
     5881 agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt cgcgctggtc
     5941 acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc cgcaacactg
     6001 atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc aatggtagct
     6061 ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg ctggagctta
     6121 caacacacca gcgacattat gggctcagcc cttcccaccg cggcggtagt gattttggtt
     6181 actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg caaagccctt
     6241 gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat tatttcactg
     6301 gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg cgggttactc
     6361 tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct ggcagcctgc
     6421 tttggtgggc ttggcgcgtc acatattaat gactcagggt tctggattgt gaccaaatat
     6481 ctggggttat cggtagcaga cggtctgaaa acctggacag tgttaacgac cattctcggt
     6541 tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta ataaaaaggt
     6601 ccgatgggca tcggaccttt tatttagcgc ttaccttccg ccagcagcgg cgggatgctc
     6661 ggcaccatcg gcgcgtcatc aatatctttc tgtgtcatgc ggaacgcttc gggataatgt
     6721 tcgcggctgg tgcggcgtag cggttcggta tcgcgccagg tatagaggca atgctggcac
     6781 tggtagaccg tccagacatc ttttaccggc gatttcgcca tcacttcaat ctgttcatca
     6841 gcacaacgtg gacaaatcat cttgttctcc ttatttacgt gcggccagca tagcggtcag
     6901 tttttcagcc caggctttgg tttccggtaa atccaccacc ggctggctgt agtgaccacg
     6961 gttgtccggg gcgacaggcg tggtggcgtc gataatcagc ttgtcggtga taccggccgg
     7021 gcttgagccg gggtcgagtt ccagcacgga catattcggc aactgcacca aatcccctgc
     7081 cgggttcact ttcgaagaga gcgcccacat cacctgcggc aggttgaacg gatcaacgtc
     7141 ttcatcgacc ataatcacca tcttcacgta gcccagaccg tgtggcgtgg tcatcgcacg
     7201 caggcccacc gcgcgggcaa agccaccgta gcgttttttg gtggagataa tcgccagcag
     7261 gccgtgggtg tacatggcat ttaccgcctg cacttccggg aactcggctt tcagttgctg
     7321 atacagcggc acgcaggtgg ctggccccat caggtagtcg atttccgtcc acggcatccc
     7381 gagatagagc gattcaaaaa tgggtttgct gcgataagag actttatcga tacgcaccac
     7441 ggtcatgtta cgcccgccgg agtagtgccc ggtaaactca ccaaacggcc cttcgatttc
     7501 gcgtttacgg ctttcgatga ccccttcgag gatcacttct gaaccccacg gcacatcaaa
     7561 accagtcagc ggggcggtgg cgatcgggta cgggctttca cgcagcgcgc ctgccatttc
     7621 gtactcggac tgatcatatt tcagcggcgt ggcccccatc agggtgatga tcggatcgtt
     7681 accgagtgtg atggcaatcg gcagatcttc accgcgttct tccgctttat gcagatgcag
     7741 ggcgatatcg tgcatcggca ccggttgcag accgagctta cgcttaccct tcacttccat
     7801 acggtagatg ccgacgttct gcttgccgaa gttatccgga tcaagcggat cgcgggaaac
     7861 cacgcacgct ttgtcgagat agaaaccgcc atcaccgtcg tttaaacgaa acagaggcag
     7921 aatatcgaac aggttgatct cgtcgccatc gacggtgttc tgcgcccagg ctggattggc
     7981 gcggcgctcc ggggcaatgg ggaagttatc ccagcggcgg ataaactcat caatctgttt
     8041 tttaaccggg gtgtttggcg gcaggccgag ggaaatcgcg tggttctgcc aggaaccgat
     8101 ggtgttcatt gccacgcggg catcgataaa gccgcgaata ttgtcaaacc acagcgccgg
     8161 tgcaacatcg ccgatacgtc cggtggcgtt ggcagcggct gccagatccg gttccgcgtt
     8221 cacctcttcg ctgattttca gtaactggcc gtggtcatca agcgcctgta aaaagctgcg
     8281 taaatcatca aatgccatta ttcattctcc tgagaaaaat tccgggcctg cggcaatcct
     8341 tgccagcgcc tggcgtgagg atgttcgagg ccaaattgat ccagcacgcg ggctaccacg
     8401 tggtggacaa tgtcatctac cgtttcggga tggttataaa aggcaggcat cggcggcacc
     8461 atcgccacgc ccatgcgtga aagtgcgagc atattttcga gatggatggt gctaagcggc
     8521 atttcacgcg gcaccagcac tagtttgcgg ccttctttga gcacgacgtc cgccgcgcgc
     8581 cctaccaggc catcggcgta accggcacgg ataccggcga gcgttttcat actgcacgga
     8641 ataacgatca tgccatcggt acgaaaggaa cctgaggaga tggttgcggc ctgatccgcc
     8701 gggttatggc agaagtctgc gagggcagca acatcgcggg cgttgtaagg cgtttccagt
     8761 tcaatggtgg ttttcgccca cttcgacatc accagatgag tctcgacatt cggcatctcc
     8821 cgcagcgctt gcagtaatgc cacaccaaga ggcgctccgg tggcccctgt catcccgacg
     8881 atcagtctca ttgtttattc tcttaattgt tcgtatacga acgttattat taaaatacgc
     8941 ctctgttttc atcgcggcaa gcactgaaaa gtaacaacat cttcttcgtc actgaaaaac
     9001 cgctatgata gcggcagata gtttgcccgg agtttacatg gcgttacgaa ataaagcgtt
     9061 ccatcagtta cgacagcttt ttcagcagca cacggctcgc tggcagcacg agttacctga
     9121 cctcaccaaa ccacagtacg cggtgatgcg cgccattgct gataagcctg gtatcgaaca
     9181 ggtggcactg atagaagcag cagtcagcac caaagcaacg ctggcagaaa tgttggcaag
     9241 aatggaaaat cgtggcttag tcagacgtga acatgacgcc gctgacaagc ggcgacgctt
     9301 tgtctggctg actgccgaag gagaaaaggt acttgctgcg gcgatcccca ttggtgacag
     9361 cgtggatgag gaatttttgg ggcgtttgag tgcagaagag caggagctgt ttatgcagtt
     9421 ggtgcgcaag atgatgaaca catagggtgc aaattgccgg atgcgacgct atcgcggctt
     9481 atccagccca caaaaacaca tgcttcccaa acaaaagaaa aaaggccagc ctcgcttgag
     9541 actggccttt ctgacagatg cttacttact cgcggaacag cgcttcgata ttcagcccct
     9601 gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt
     9661 cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttcg taccccagca
     9721 aaccgaatcg acgtgccagt acttcacgct gtttggcgtt cagctcgaac agccatttga
     9781 cgatgctctg cttcatatcg tcatcttgcg tggtatcttc cggaccgttc tctttttcat
     9841 cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg
     9901 taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct
     9961 ctgcgatctc ttccgcactt ggttcgtggt ccagtttatg ggacaactcg cgtgcggttc
    10021 gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt
    10081 tcataatcgc ccgttcgatc gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga
    10141 aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaggttg ccctcttcga
    10201 tcaggtccag caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac
    10261 gcaagttact ctcgatcatc cggcggcggg aggcaacatc tccacgcagt gcgcgacgcg
    10321 caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa
    10381 gctgagtcgc gtccaacaca cgctgtgtgg ctccctgcga taacagttcc tcttcggcca
    10441 aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt
    10501 tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa
    10561 ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat
    10621 ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa
    10681 atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt
    10741 cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc
    10801 atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac cggcataaac
    10861 aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcccgcga tatcaatccc
    10921 cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg
    10981 ccaggtggag ataggcgtac tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc
    11041 tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat
    11101 tttgttagca ctctgttcac ccgaagactc agaatacgta attgtcggtt gcgaagcaac
    11161 agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg catcggcctg
    11221 ggtaatggca tttccgccag tgattggcgt accggaagca ttacccacct gtaaggtctg
    11281 accgacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc
    11341 gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg tataggtact
    11401 gccgctataa ctgcctttcg gaatgttccc atactgacgg ttgtagacga tgcgtccgtt
    11461 ttccatctgt accggctgct gagctactgg ctgtactggc tggatttgcg gttgttgagt
    11521 agcctgaatt tgtggctgct gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt
    11581 tcccattttc ggcggcggcg taatcaacat accagaattg gtatttgcag gcgcattgcc
    11641 attaacggag ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg ccagccatag
    11701 cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc ccgcgctcat
    11761 ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca aggcaccata
    11821 ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg gcaaaataac caggaaaaat
    11881 ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca aagcgcacgg
    11941 cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc aaatactggt
    12001 gctcctcccc tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc attagtgcag
    12061 ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc
    12121 aaccttgcca tccatcgcca tgacgggttg aaacattatg taaatcaaga tttttcaggc
    12181 ggcgacgcgc ctgccactgc aaacctttaa tccgttcgac cgagcaaaca tgctggacaa
    12241 gatgcgccag gattgccgtt tgatatcccg aaccggtgcc aatttccagc acccgcgact
    12301 gcggcgtcag ctcgagtaat tcggtcattc gcgccaccat atacggctgc gaaattgtct
    12361 gcccctgacc tatcggcaag gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa
    12421 cgaatttttc acgcggcacg gcggcaagtg cattcagcac ctgctcatcc tgaatacctt
    12481 gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg tgccaactcc
    12541 cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg
    12601 cagcggcgtg atggagacat agccctcatc taccgcagca aaatcggtcc ccggaccggc
    12661 atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg
    12721 cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat
    12781 ttgatccaat ggtaaatccg gaacgttaat attaagaata cgtccggtgc gcagcggctc
    12841 tttacacagt gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg
    12901 cccgtcaagc gagacggcaa gcgccggaaa acctaaatga cggccttcca tcgcggcggc
    12961 taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa ttccggacac
    13021 aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacgc aatcggtcgg
    13081 ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca gggaggattc
    13141 cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc
    13201 agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc
    13261 attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg acaacgctgg
    13321 ttgcaaaact acccgccgga agccagaaac ggatttctac ggtgacgtca tcccaccagt
    13381 tccagcttaa ttgttgcgga tacagcagca tcgctctgcg cgcggcttca actttttcgc
    13441 gcaccagtaa ggcttgtaat tcagtttctg cggcgacagc tgcttgttcg aatgccaacg
    13501 cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc atcagctctt
    13561 tatcgttgac gcgacgctgt aattccgcca gttcttcggt ggttgcgaca aaccagctac
    13621 cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct gcttttttga
    13681 ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac caaaaactcc
    13741 gtttattgcg atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt
    13801 tgctaccgcc aatcccaaaa cgttgggcac caaaatagtt aggcacgcct tttacgcaaa
    13861 tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgcaga accagggtaa
    13921 aggcgttacc tttcagcgcg cctaaacgca gcttgcgctt gtgccgcgca tactccagca
    13981 cctggcagcc ttccagttga aaggcgctca gatcgggcat ttccttgccc ggcacgcgag
    14041 cacataacca ctgttccgtt acagcatgtt tgtctttttg cccagcgaag ctgacttcac
    14101 gggcatgaat tttaaggaat ttcgccagtg catccgccac aaaacgggta ttgcagccgt
    14161 ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag cccaaatctt
    14221 ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc ggtttaccgt
    14281 ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta gcgccaccgc
    14341 ttcacaggca atcccttccc cacgtccggt aaatccaagt ttttccgtag tagtggcttt
    14401 cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc gcatttgtgg
    14461 aatgtgcggc aacatcttcg gtgcctgagc gatgatagtg acatcgacgt tgcccagggt
    14521 ataacccttc gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc
    14581 tttaaatgcc ggatcggtat ccgggaacag tttgccgata tcccccagcg ccgccgcgcc
    14641 aagcaatgca tcggtcaacg catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc
    14701 tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc caaaggcgtg
    14761 tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt tcgggtgagg
    14821 taaaactcgg ccagtgccaa atcttccggg cgcgtgactt taatgttatc cgcgcggcct
    14881 tcgaccaact gcggatgaaa tccgcaatat tccagcgccg aggcttcgtc ggtaatggta
    14941 gcgccttcat ttagagcgcg cgtcaggcag tcatgtaaca gctcacgagg gaaaaattgc
    15001 ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc atttttgccc
    15061 ggttcggcac gtttcatcgt atcgcgcact ggtgcggcga ggatcccccc cgtgcggctg
    15121 gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat gcagacaagg acgagctgcg
    15181 tcatgcacca atacccactg cgcgtcgcca gcggctttca gacctgccag caccgaatcg
    15241 gcacgctcat caccgccatc tacaacggtg atttgcggat gattcgccag aggaagttgt
    15301 gcaaaacggc tatcgccagg acttatggca atgacgacat gtttcacccg gggatgcgcc
    15361 agcagcgcat gcaccgagtg ttcaagaatg gtttgattac cgattgagag atattgctta
    15421 ggacattccg tttgcattcg acggccaaat ccggccgccg gaaccacggc gcaaacatcc
    15481 aaatgagtgg ttgccatgtt aattcctggg ctgatttatc gattgttttg ccccgcagac
    15541 tgtgcgcgct tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg cctggtcatg
    15601 ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag atcgtcaatt
    15661 tcggcaaaaa gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc
    15721 acatcatcat tgacgcgggt atagtcatgt ataccgttct taccgaacca cagcgaatac
    15781 tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct gccccctgaa
    15841 aaacggcatc atcatcccat gcatccgaag acgactctac atcctctgtt ggggataccg
    15901 cgacaacgcg ggcaaatgta ccacatttgt ccattgttac gtatacccag ggcgtgcaga
    15961 acataatctc attattagtt acggtttgaa ttatgaacag aggagacaag aaagtacaaa
    16021 ttagcccagt agccacataa acagtgcgcc aaacataatg cctactgtca ccagggtgaa
    16081 aacaatactg tagcgtagct ttccgtccat caatgaatgc agcgcaatcc ccaccactac
    16141 cgcgacaggc atcagtgcca gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt
    16201 agagccataa atcaacatcg gcatcgccag cgcaaataac catgagataa aaccgatcac
    16261 ggcaccaggc agtgaccatg tggtttcttc atcctcagta aggctgtcgt tatttgttag
    16321 tgtaatgtta tggctattac gcatatttga tcctgttact ttgacgaacc gggcatggaa
    16381 acccggtggt gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct
    16441 gtaccaaatt tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggcgctt
    16501 cgtaaacgga atctattccc gtaaagttac gcagttcacc ggcacgcgct ttcttatata
    16561 agccttttgg atcgcgggct tcgcaaatcg ccagcggtgt atcgacaaac acttcgataa
    16621 agcgcccttc tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa
    16681 atgcggtcag caccaccagt ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac
    16741 ggatattttc tttacgatcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa
    16801 cattgtcgcc atccagcaga tacgtactga cgccgagttt atgtaacgcc tcctccagcg
    16861 ccccggcgac cgttgattta ccggacccgg agaggccggt aaaccacagc actacaccac
    16921 gatgaccgtg gtgtagctcg cgttgttgca cagtgaccgg atggctatgc cagacgacgt
    16981 tttcgtcatg cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa
    17041 gtggcgacga accagtgcat tcaattccag ttcgaatgca ctgaattcag atggcgcagt
    17101 agttgcctgg ctaactggct cgtgcaccat accggcacct acggtcacat tactcaggcg
    17161 atcgataaaa atcagcccac cggtaaccgg gttttgctga taacgatcta acaccagtgg
    17221 ctcgtcaaag gtgagatcca ccaagccgat gccgttcagc ggcaggtttt caacttcgcg
    17281 ttgggtaagg ttattaatat caacctgata acgaatgcca tcaacacgag cacgcgtctt
    17341 cttaccggca attttgatgt cgtaacgctg gcctggggaa agcggctgtt ccgccatcca
    17401 taccacatcc accgacgcgc tctgcacagc cggtaatgat tcgtctgccg ccagcagcag
    17461 atcgccacgg ctgatgtcga tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa
    17521 ggcttcttcg cgatcgccat caaaagtcac gatccgcgcg acgtttgatt ccacaccaga
    17581 gggcagcact ttgacacgtt gcccgacttc cacgcgaccg gatgccagcg ttccggcgta
    17641 gccacgaaaa tcgagattcg ggcgattaac gtactgcacc gggaagcgca ttggctgggc
    17701 atccaccact cgctggatct ccacggtttc cagcacttcg agcagtgtcg gaccgctgta
    17761 ccacggcata ctttcacttt gcgaagccac gttgtcgcct tccagtgcag agagcggcac
    17821 aaagcggata tccagattac ccggcagctg cccggcaaac gtcagataat cttcgcgaat
    17881 acgggtgaac gtctcttcac tgtaacccac cagatccatt ttgttgatcg ccacgaccag
    17941 atgtttgatc cccaacagtg tggagataaa actgtgacga cgggtttgat cgagcacgcc
    18001 tttacgggca tcgatcagta agatcgccag ttcacatgtc gatgcgccag tcgccatatt
    18061 gcgagtgtac tgctcgtgcc ctggggtatc ggcgataata aatttacgct tctcggtaga
    18121 gaaataacgg taagccacgt caatggtgat gccctgttcg cgctcagctt gcaggccgtc
    18181 caccagcagt gccagatcca gtttttcgcc ctgggtgccg tgacgtttac tgtcgttatg
    18241 cagcgatgag agctgatctt cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag
    18301 ggtacttttg ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg
    18361 ttgcgcaatc atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtgcggt
    18421 gttcatctta aaaatacccc tgacgttttt tcaactccat ggatcccgcc tggtcgcggt
    18481 caatcacgcg accctgacgt tcactggtgg tggaaaccag catctcttcg atgatttccg
    18541 gcagtgtttg tgcatttgac tccaccgcac cggtcagcgg ccagcagccc agcgtacgga
    18601 aacgcaccat ccgtttttta atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa
    18661 tcatcatcaa cataccgtcg cgttccagaa ccggtcgttc cgcagcgaga tacagcggaa
    18721 caatgtcgat attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga
    18781 gcgggaagac gcgaatgctt tcgcctttgt taatttgccc gttgtagttg tgccacagct
    18841 ccgggcgctg atttttcgga tcccagcgat ggaagcggtc acggaaagag tagattcgct
    18901 ctttagcgcg ggatttctct tcgtcgcggc gtgcaccacc gaaggcggca tcaaaaccgt
    18961 atttattcag cgcctgtttc aggccttcag ttttcataat atcggtatgt ttcgcgctgc
    19021 catgaacgaa tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct
    19081 cgcagccgta ggctttagcc gtacgatcgc ggaactcata catctcgcgg aatttccagc
    19141 cggtatcgac atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca
    19201 gatgcagcat gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt
    19261 ctgccgccac ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aggtgagtaa
    19321 gtcgtatttg atccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt
    19381 tcgactatag ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta
    19441 acggaataac gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc
    19501 gtttaggaag atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa
    19561 aatttgcatg gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc
    19621 ctggcgctcg gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat
    19681 tttgctaata ctcaggcacg acatattgct actttctttc cgggacgcat gaccggaact
    19741 cctgcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc
    19801 agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagagc
    19861 tggcataacg tgacgggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag
    19921 atcatcatta tggcgcatct ggatacatac gccccgctga gcgatgctga cgccgatgcc
    19981 aatctcggcg ggctgacgtt acaaggaatg gatgataacg ccgcaggttt aggtgtcatg
    20041 ctggaactgg cagaacgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg
    20101 accagtggtg aagaggaagg gaaattaggt gctgagaatt tactcaagcg gatgagtgac
    20161 accgaaaaga aaaatacgct gctggtgatt aatctcgata acttaattgt tggcgataaa
    20221 ttgtatttca acagcggtgt aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg
    20281 gcgctggcaa ttgcgcgtag tcatggaatt gccgcaacga ccaatccggg tttgaataaa
    20341 aattatccga agggtaccgg gtgctgtaac gacgcggaag tttttgacaa agccggtatt
    20401 gcggtacttt cggtggaggc aactaactgg aatcttggga ataaggatgg ctatcagcaa
    20461 cgtgcaaaaa ccgccgcctt ccctgcggga acaagctggc acgatgtcag gcttgataat
    20521 caacaacata ttgataaggc gcttcctgga agaatagaac gtcgctgccg tgatgttata
    20581 cggataatgc taccgctggt gaaagagctg gcgaaggcgt cttgatgggt ttgaaaatga
    20641 gagctgggag ttctaccgca ggggcgggga actccaagtg atgtccatca tcgcatccag
    20701 tgcgtccggt ttatccccgc tgacgcgggg aacacggctt taaccacacg agcaagtcct
    20761 tcgctggcgg tttatccccg ctggcgcggg gaacacagtt aacaacccgc gccgaacaag
    20821 cccgacaacg gtttatcccc gctggcgcgg ggaacactcg caccgcgtta atccggcaga
    20881 aaaacaacac ggtttatccc cgctggcgcg gggaacaccc gacaatctcc gcctccagtc
    20941 ggtcgacctg cggtttatcc ccgctggcgc ggggaacact tcgccgccat catcctgtgg
    21001 tgccagttga ccggtttatc cccgctgacg cggggaacac gcgcctcggt tgtttagcca
    21061 tgctcagccc cacggtttat ccccgctgac gcggggaaca cctttccacc caggcactga
    21121 tactccacat aaacggttta tccccgctga cgcggggaac actccccctt aattgctctc
    21181 aaaagatttg atcccggttt atccccgctg acgcggggaa cactcacact cacgcggagt
    21241 gagacggcgt acgagcggtt tatccccgct gacgcgggga acacgatcgc cgggcgcggt
    21301 tacccgctgc gaaaatcggt ttatccccgc tgacgcgggg aacacctcac gcgaggcatc
    21361 gatcgtgatc tgat
//
LOCUS       DARNVM010000075        21148 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.075, whole genome shotgun sequence.
ACCESSION   DARNVM010000075 DARNVM010000000
VERSION     DARNVM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.075"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(188..589)
                     /gene="nikR"
                     /locus_tag="SI621_002550"
     CDS             complement(188..589)
                     /gene="nikR"
                     /locus_tag="SI621_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEI4065254.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(594..1400)
                     /gene="nikE"
                     /locus_tag="SI621_002551"
     CDS             complement(594..1400)
                     /gene="nikE"
                     /locus_tag="SI621_002551"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HEI4065255.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAYPVRRRATEKV"
     gene            complement(1397..2161)
                     /gene="nikD"
                     /locus_tag="SI621_002552"
     CDS             complement(1397..2161)
                     /gene="nikD"
                     /locus_tag="SI621_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HEI4065256.1"
                     /translation="MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2161..2994)
                     /gene="nikC"
                     /locus_tag="SI621_002553"
     CDS             complement(2161..2994)
                     /gene="nikC"
                     /locus_tag="SI621_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HEI4065257.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2991..3935)
                     /gene="nikB"
                     /locus_tag="SI621_002554"
     CDS             complement(2991..3935)
                     /gene="nikB"
                     /locus_tag="SI621_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HEI4065258.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3935..5509)
                     /gene="nikA"
                     /locus_tag="SI621_002555"
     CDS             complement(3935..5509)
                     /gene="nikA"
                     /locus_tag="SI621_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEI4065259.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5620..6207)
                     /gene="acpT"
                     /locus_tag="SI621_002556"
     CDS             complement(5620..6207)
                     /gene="acpT"
                     /locus_tag="SI621_002556"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HEI4065260.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLSADSVQWIDSVN"
     gene            complement(6262..7311)
                     /locus_tag="SI621_002557"
     CDS             complement(6262..7311)
                     /locus_tag="SI621_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEI4065261.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHISPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7443..8660
                     /locus_tag="SI621_002558"
     CDS             7443..8660
                     /locus_tag="SI621_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065262.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8664..9221)
                     /gene="dcrB"
                     /locus_tag="SI621_002559"
     CDS             complement(8664..9221)
                     /gene="dcrB"
                     /locus_tag="SI621_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HEI4065263.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9294..9959)
                     /locus_tag="SI621_002560"
     CDS             complement(9294..9959)
                     /locus_tag="SI621_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEI4065264.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10181..10426
                     /gene="tusA"
                     /locus_tag="SI621_002561"
     CDS             10181..10426
                     /gene="tusA"
                     /locus_tag="SI621_002561"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HEI4065265.1"
                     /translation="MTDLFSCPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10528..12726)
                     /gene="zntA"
                     /locus_tag="SI621_002562"
     CDS             complement(10528..12726)
                     /gene="zntA"
                     /locus_tag="SI621_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HEI4065266.1"
                     /translation="MSTPDNHGKKAPHFAAFKPLTTAQNANNCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQTSRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAIALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPAVAFAGLINELESAGQTVVLVVRNNDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12800..13426)
                     /locus_tag="SI621_002563"
     CDS             complement(12800..13426)
                     /locus_tag="SI621_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEI4065267.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13567..13926
                     /locus_tag="SI621_002564"
     CDS             13567..13926
                     /locus_tag="SI621_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HEI4065268.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13929..14198)
                     /locus_tag="SI621_002565"
     CDS             complement(13929..14198)
                     /locus_tag="SI621_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HEI4065269.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14188..14784)
                     /gene="rsmD"
                     /locus_tag="SI621_002566"
     CDS             complement(14188..14784)
                     /gene="rsmD"
                     /locus_tag="SI621_002566"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HEI4065270.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14934..16430
                     /gene="ftsY"
                     /locus_tag="SI621_002567"
     CDS             14934..16430
                     /gene="ftsY"
                     /locus_tag="SI621_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEI4065271.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPELVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16433..17101
                     /gene="ftsE"
                     /locus_tag="SI621_002568"
     CDS             16433..17101
                     /gene="ftsE"
                     /locus_tag="SI621_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEI4065272.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17094..18152
                     /gene="ftsX"
                     /locus_tag="SI621_002569"
     CDS             17094..18152
                     /gene="ftsX"
                     /locus_tag="SI621_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEI4065273.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18397..19251
                     /gene="rpoH"
                     /locus_tag="SI621_002570"
     CDS             18397..19251
                     /gene="rpoH"
                     /locus_tag="SI621_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEI4065274.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19522..20625
                     /gene="livJ"
                     /locus_tag="SI621_002571"
     CDS             19522..20625
                     /gene="livJ"
                     /locus_tag="SI621_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HEI4065275.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20825..>21148)
                     /gene="panM"
                     /locus_tag="SI621_002572"
     CDS             complement(20825..>21148)
                     /gene="panM"
                     /locus_tag="SI621_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HEI4065276.1"
                     /translation="KIWPEYSPSSLQVDDNHRIYAARFNERLLAAVRVTLSGTEGALD
                     SLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGVEDRGVMTAFMQALGFTAQQG
                     GWEKR"
BASE COUNT         5012 a         5585 c         5873 g         4678 t
ORIGIN      
        1 tattatttta atgacagtat cttcatacga aaaagcgaag aaaaacttgt ggtcacaatg
       61 aattgaaggt ttttaggaat atatttagaa cgttacaaaa gagaaggttc ggatagggta
      121 tttagagagg aatcaggtgt gctagtggcc ctttgttgat cgttcaccaa aggacccaat
      181 aataggctta atcttccttc ggcaagcact gcaaatgccc gtgccgcacg ccgcgctggg
      241 cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac acggcgattt
      301 ccagacagtc gtcgtgattg atgtggacat gtagcgtcgc gacggagaga tcgtggtgat
      361 ggtgctgggt ggagacaata cggctggcta agtcgcgttt ttcgtgttca tacacatacg
      421 acagcaccgc gaaaccttgt gtgccgtgct gctgagtggc ctcttgcgcc agggcgctac
      481 gcagaatatc gcggatagct tcggaacggt tgttatagcc acgacgctgg ctcaggctgt
      541 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtaac tcgttgcata gacttaaacc
      601 ttttctgtgg cgcgacggcg cacggggtat gcgggtaata ccgcgttttg tagcacacgt
      661 ccggcgtcag aggaaaaggt taatttatct cccaccacct gggtttcgac gatttgcccg
      721 ttgtccatca ccatcactcg ctggcaaaag cgttccacca ggcgtaagtc atgagtgatg
      781 aacaggcagg cagtgccaaa ctgttgttgt agctttttca gcaggcgaat gacgcccgcc
      841 tgtaacacga gatcaaggtt agaaacggct tcatccagaa tcagcagttt cggttcgacc
      901 gccaacgcgc gtgccaggca gacgcgctgg agctggccgc cgcttaactg cggtgggcgc
      961 ttgtcgagca cgctgtcatc gagatcgacc gccttcagca tttcgctggc gcgcgccagt
     1021 tgttcggctt ttttcagcga tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg
     1081 gttttgcgcg gattcacggc actgatggag tcctgaaaca ccatctgaat atcacggcgg
     1141 aaggctttac gttgggcgcg attaagtttc gccagcgatt cgccgcgcca gctaatattc
     1201 ccttgtgagg gagactctaa gcctaccagc agccgcgcga gggtgctttt tccgcagccg
     1261 ctgcgtccca gcagggcgac ggtttcaccg cttttcaggg tcagggaaac gttattcagt
     1321 accgcctgat gctgatgttt tccgctaaat ccaccgtgcg catagtgatg tgaaaggtcg
     1381 cagacgttaa gtaaagtcat gatgccagct ccataccgta gagggcgaga tgagcggaaa
     1441 ccaggctgcg cgtcaccgta tgtttggggg cgttaaacag cgtttctaca tcgccctgtt
     1501 cgacaatttt accgtgtgac atcaccgcca cgtcatccgc cagacgcgcc acaacgccca
     1561 tatcatgggt gaccagcagc attcccggtg cttgtttttg cataatgctt tccagcagat
     1621 cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt cggttcatcg gcgatgataa
     1681 acggcgattc acacaacacc gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca
     1741 tctcgaacgg gtacagcttc aacacgcgcg cggcgttttc caaccccacc gcttctatgg
     1801 cagcggtaag cgtagcgtca tcggcgggtt tccccagcgc caggcaggtt tcacgcgcgt
     1861 gggtgtgcat ggtgtgtagc ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa
     1921 ttttgatgcc gcgcagggca cagggagaaa ccggtttgcc atcggctaaa atttcccccg
     1981 ccgtctggcg aacgcctgcg ggcagaatgc ccagcgtcgc ggcgcaggtc agtgatttcc
     2041 cgctgccgct accgccgact aacgccagca cacgcccgcg tttaagggtt aacgatacgc
     2101 cgtgcaccag cggctgcgcg gcctgtagcg cgatatcgcg cagttcaatc tgttgcggca
     2161 tcagtgtgcg tgctccgtca ccagatgagg atccagatga tcgcgcagtg cgtcacccac
     2221 caggttaaag gccatcacgc tgataaacag cgccagcccc ggccagaaca tttgcagcgg
     2281 ctgggtccag atatactgac gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc
     2341 ggtcacgccg aggccgagga aggacatccc cgcgacgtgg agcatcatat gaccaatatc
     2401 cagcgtcgcc agcaccagca gcgaggggat caccgcgcct gccagatgat cgacaaacac
     2461 ccgcacatgg cccgcgccgg aaagccgtga cgccagcaca aactcgcgct ggcgtagcga
     2521 aatcaccagg ctgcgcacca tgcgcgcata ccacgcccag tgcgacaggg cgatggcgat
     2581 aatcacgttg gtcaacccgg tgccgagtac gccgaccata aagaacgaca gaatcgacgt
     2641 cgggaaggtc ataaacatat ccgcgacgcg catggtggcc tgatcaacgc gcccgccaat
     2701 cagcccggcg ctgccgccaa taactagccc taatgtcagc accagcagca ggcaggccat
     2761 taccgagccg agcgacacgc gggtcgccgc catcagccgc gagaaaatat cgcgccctaa
     2821 atgatcggta cccagccagt gctgcgcatc cggtgaaagc aggcgcgacg gcagatcaat
     2881 cgcctggggg tcatacggca gccaccactg gctggtgagc gcaatcagcg ccagcagggc
     2941 gataatgatc agcgccaggc gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgg
     3001 gctccttcat gacgacgaat gcgcggatcc agcgcggcgt tgagcaaatc aacaatcaaa
     3061 ttacagacca caaaaaccac caccatcatc agcgtaaagc actggatcac cggatagtca
     3121 cggttaaaaa tcgccgacac cgcatagcgc ccgacgcctg gccaggcaaa gatgttttcg
     3181 ataatcatcg tcccgccaat cagttcgcca atgtgcatcc ccacggcggt gatcatcggc
     3241 agcgaggcat tgcgcagaat atggcggcgt tcggtctgtt tgtcgttcag gccgcgtagc
     3301 cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca tactggcgcg cagtagccgt
     3361 gcattaatcg ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag
     3421 ccgccgtagc ccatcgcggg tagccattgc agatataccg aaaacgccat caccagcaaa
     3481 aacgccagcc agaagttggg catcgacacg ccgagaaacg cgatgaaacg tacggcgaaa
     3541 tccggcagac gatcgcgatg gcgcgccgcc cagataccga gcggcacgga agtgagcaga
     3601 attaatacca gcgcggcacc tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg
     3661 tccagcaccg ggcgttggct ggcgaatgag ataccaaagt caagatgcag cgccttccac
     3721 aaccaggtgc cgtactggac atacagcggc tgatccagcc ccagcatggt gcgtgtagag
     3781 gccagcatct ccggcgtcgg cggcaggtta gacagacgca gataatcgag cgccgggtcg
     3841 ccggtgccga ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac catcgggatc
     3901 agcagcagaa agcggcgtaa tacgtaacgc aacattaagg tttcaccggt ttaatctgtt
     3961 cgaacggaat ttcggtggcg atcggcgcgt aggggatgtt acccagctcc ggttttgata
     4021 ccaccatcat tgagatgtaa ctgataggca gataaaccgc ctcgtcatgc agacgggtca
     4081 gaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg ggttgccagc acttcgccga
     4141 tctctttatc aatcagcggc ttgtcggcta atccttgctg tgcctggaaa tcagcgtgcg
     4201 acggtacgcg cattgaactc aggaaggcgt gtggatcgta tggcgcgccc caggtgcgat
     4261 ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata gatactgctc tcttcttcgc
     4321 caatcagtga gacatctgcg ccaatctggc gcatatcagc ctgaatgatt tccgccatcg
     4381 atttgcttaa cgcatcggtg ccgatgaacg acagttcaat gcgcagcggc tgaccatttt
     4441 tctcgcggat gtctttgccc gcaggcaacg tccaaccggc tttttccagc aacgctttcg
     4501 ctttctgcgg atcgtactgg cgcggtttca ggccgaggtt ggcatagggc acagaaggag
     4561 caaacagggt gtcggcgacc tgctgggtgc catacaacgc gttatcaatc agcgattttt
     4621 tgtttaccgc gtaattaaga gcttcacgta ctgccagctc gttggtgggg gctttggcgg
     4681 tattgagcgc cagcatcacg gtttcgatcg gctgtgacag ttgggtgtgg taagccgggt
     4741 tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt
     4801 caatatcgcc agtttcaaac gccaccgcgc gggtagtcgg gtccgggatc acgttaaaag
     4861 tgatcttctt aatcgccggc ttttcgcccc agtagttttc gttacggacg aagacatcgt
     4921 actgattcag tttcgattcc tgcaaaaccc acggcccggt gccaatcggc gctttaattc
     4981 cgttcatggt ttcatggttt ttaaactgcg agggggcgat aaaacggaaa gggcgtggca
     5041 gggccagttc ttgcaggaaa ggatagtagg cgcttttcag ggtgatttgc agctctgttt
     5101 tactgagtgc tttgacatca acaatctggt ttgccagctc cagccaggcg tgacgttgac
     5161 ggttatcgag cactgcgcgg aagttttctg ccgccgcctc tgcatcaaac ggttcgccgt
     5221 tggagaattt cacgtcatca cgcagggtga aggtccaggt tttaccatct tcagaatgag
     5281 tccagctttt tgccagccac gggatcaccg aaccgtctgc ctgatatttc accaatggtt
     5341 cataaaccat gctctgggcg aacatctggt taggcgtgta aaggtgcggg tttagtggcc
     5401 cgacattcac cggccaggcg gtggtgattt catctggtgc agcggcatgg acgataaaag
     5461 acgcacaagc cagcagcgca aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa
     5521 tggattaaaa tagatggcgg aaataagtat gacgatttta agtattcgtc atactgataa
     5581 cctgttctgg atcaaaagat ggacagtcgg gtgggcggat cagttaactg aatcgatcca
     5641 ttgcacactg tcggcggaga gcgtaaaggg ggtaggggtg cagatcgcca ggcttaaatt
     5701 atcaagctgg caatggctga ccgacagtga tgagtgatag gtgctgtcta cgctgacgat
     5761 ttgccaggcg ctgccgccac gttgtttaac gatggcttct ttgcgcgtcc agatgcgcca
     5821 gaacatttcc agttgctgat cgggatgcac ggcgtccatc tcagcgtgtt ccccgaggct
     5881 gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg cgaattactt cgatatcgca
     5941 gccgacttcg ccttcatcgc tcaacagcag ggcgatatcg tcaccgctat ggcttaagtt
     6001 gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct tgttcgccat agatgatctc
     6061 cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc gttcacgtcg
     6121 tggaccttgc ggtgcttgct cgcgtaaacc cggtggcaga ggagctgcgc ttaaggtcga
     6181 aactttcccc agaactatcc gatacatatc agggccaacg tttaatggaa aatgaaagtg
     6241 cgtatcgtaa cacttgtcgc ctcatcccgg taaccgactt ttcggtctgc ctggtcccag
     6301 taaaatcgcc agtttgctac cgcctttggt ggtttccatc cagattttac acacgctggt
     6361 taatggcacc gaaagtagca tccctaccgg gccgagcagc catccccaaa ttaacaatga
     6421 aagaaatacc accatggtgg acatccccag gcgatggccc atcatccgtg gctctaaaat
     6481 attgccgatg accatatgga caactaaaaa caatgcgccg accagaatac attcgtaaat
     6541 accattaaac agcagcacct gaatcattgg cggtacggcg gaaattaccg cgccgatatt
     6601 gggcacgtag ttgagcaaaa acgccagtac tgcccacatc agcgcaaact gtacgcccat
     6661 tagcgccagc cccagccaga cgatgacacc tgtccataaa ctgagtaacg tctttaacgc
     6721 cagataatgc gaaacgcctt taagtgcgcg gtgtaatccc gcgatgtgaa tctgtgggtt
     6781 atttagcgca aaacgcattt tgtaagggac gtggcgcact tcaaacagca tgaaaactac
     6841 ggtcatcacc agcaaaagca cgctcgccat tgccccggaa agcccggtca ttagcgccgt
     6901 ggtgaaggta accacttttt ccgagtccat ccgctgcagt attcgctccg gcgatatatg
     6961 caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt ttgcgcgtca gctccttatt
     7021 aaacttcggc agcatagata taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc
     7081 ggttagtgcg atcagcatca ccaccaccac aatcgtaatg gcaacggggc gttgtacccc
     7141 ccgacgaata aaccaggtga cgagcgggtt gagaacgatg gcaaaaaaca gcgccagtaa
     7201 tagctgcaca atgatatctg ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc
     7261 cagcttgagc agaatgtgca tgcccgtttt atcgggttga ggggtttcca ttggggcttc
     7321 cttgtgactt tttgtattaa gtgtagcggg agtcacgcca gcaatattct gattcttact
     7381 gctgattttc acgtcagcac gtggtaaaaa tgaaacactg ttgtaaaaat gtggtgatcc
     7441 tcatgcccga acccgtagcc gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg
     7501 tctccattgt catgtttaac ttcgccagct acctcaccat cgggttgccg ctcgctgtat
     7561 taccgggcta tgtccatgat gtgatgggct ttagcgcttt ctgggcagga ttggttatca
     7621 gcctgcaata tttcgccacc ttgctgagcc gccctcatgc cggacgttat gccgatttgc
     7681 tgggacccaa aaagattgtc gtcttcggtt tatgcggctg ctttttgagc ggtctgggat
     7741 atctgacggc aggattaacc gccagtctgc ccgtcatcag cctgttatta ctttgcctgg
     7801 ggcgcgtgat ccttgggatt gggcaaagtt ttgccggaac gggatcgacc ctatggggcg
     7861 ttggcgtggt tggttcgctg catatcgggc gggtgatttc gtggaacggc attgtcactt
     7921 atggggcgat ggcgatgggt gcgccgttag gtgtcgtgtt ttatcactgg ggcggcttgc
     7981 aggcgttagc gttaatcatt atgggcgtgg cgctggtggc cattttgttg gcgatcccgc
     8041 gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt tcgcgcggtg cttgggcgcg
     8101 tctggctgta cggcatggcg ctggcgttgg cttccgccgg atttggcgtc atcgccacct
     8161 ttatcacgct gttttatgac gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt
     8221 ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa cggcattaac cgtatcggcg
     8281 gcttaaacgt agcgatgatt tgctttagcg ttgagataat cggcctgcta ctggttggcg
     8341 tggcgactat gccgtggatg gcgaaaatcg gcgtcttact cgcgggggca gggttttcgc
     8401 tggtgttccc ggcattgggc gtagtggcgg taaaagcggt tccgcagcaa aatcaggggg
     8461 cggcgctggc aacttacacc gtatttatgg atttatcgct tggcgtgacc ggaccactgg
     8521 ctgggctggt gatgagttgg gcgggcgtcc cggtgattta tctggcggcg gcgggactgg
     8581 tcgcaatcgc gttattactg acgtggcgat taaaaaaacg gcctccggaa cacgtccctg
     8641 aggccgcctc atcatcttaa aacttactga ataaccagcg tattaatgat gttttctgcg
     8701 gtggtctgcg ctttttgctg atcgtcagct ggcagagtaa tttgcatggt cagcagttga
     8761 ttacccacgt tacccagaat aacggaagag tacgccgtct ggcctttcgc ggagataatg
     8821 ctgtctaact gctgcatttt gtgacctttc agctcaatgg ctttattggt taccacttgc
     8881 agctgcggat cgcggctacg ttgctgatct tccagacgct tcgccagcac cgccagatct
     8941 tctttcggat catcgcccat gatgacgatg actgctttct gcccggtggc gtcggaccag
     9001 acatgcatgt tattggcctg cgttcccagc ttaccgctct ggtcggtcat atccgctggc
     9061 agcgagaaac ttaacttgcc atcaagcagg ttgacgggat tcccggtagc gttactttcc
     9121 gcgaccgaac cctgcgccgt agcgttagtg tctttatcat cacaggccgc aagccccata
     9181 accagcaggc caattccgac atatttaacc agattgcgca ttgacttctt cctttcgata
     9241 aacggccata acggctcatt catccatctt atcacaactc tgataacgaa cctttaactc
     9301 gcctgcaaag cgttgatttc ggatttatct gccagtcttt tcaacagcat attgagtaat
     9361 acgccataca ttggcaggaa gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc
     9421 agtgcgattt ccatccagtg ttcagccata aaggcatccg ggctacgcca gaaggcaatg
     9481 aagaagaagg ccagcgtgtc gctgacgtta ccgaacagtg tggacgctgt cggtgccagc
     9541 caccagcggc gactctgacg caggcggtta aaaacgtgca cgtcgaggat ctgtcccagc
     9601 gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa acaggttgaa gtgggcgagc
     9661 gcgccgaatc cttgccatga ccccatataa aacagcgatg agatgacgta ggagatcagc
     9721 aacgccggga tcatcaccgc aaagatgata cgacgggcca gcggtgcgcc aaaaatacgc
     9781 acagtcaggt cggtagcaag gaaaataaac ggaaagctaa atgcgcccca ggtggtatgg
     9841 aaaccaaaaa tggagacggg aagctgaacc agatagttac tggaggtgat caccagcaga
     9901 tgaaataacg ataaccagaa caacgcctta tagcgttgag tttgcgagaa aacgttcata
     9961 ttgtaccttt ttgattaacc attggggtga gggaacccaa tacgtacgac acgctatttt
    10021 accttgatta aataacgttc ttaccctttt ttcgagccgc cgcatgatac tgctttacgt
    10081 tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac ggactcacag
    10141 ggcgtaaact agcgccgttt ttttatgtga tgagaagaaa atgaccgatc tcttttcctg
    10201 ccctgaccac acactcgacg cacttggcct gcgctgcccg gaaccagtca tgatggtgcg
    10261 caaaaccgtg cgcaatatgc agccaggtga aacgttgctg attattgctg acgatccggc
    10321 gacgacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg ttgcgaaaga
    10381 gacggatgga ctgccttatc gttatttgat tcgcaaaggc ggttgatggg gaaagattgg
    10441 cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga ggggaccgat
    10501 cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta acgcattcgc
    10561 tgtcaccagc accgtcgcac ccgtatctgc cagcaccgcc agccacaggc cggtcatccc
    10621 taacagtgtg gtgacgagga agatcccttt cagccccagc gcaatagtga tgttctggcg
    10681 gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc gcaggtggtt
    10741 atgggttaat gctgcatcgg cggtttccag cgccacgtct gtgccactgc ccattgcaat
    10801 cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga ccatcgccag
    10861 cggcgcatgt tgattcagct cggtcaccgc tttgacttta tcttccggca acaggcccgc
    10921 tttaaattcc agccccagtt ccccggcaat tgccgccgct gcgcgtggat tatcgccggt
    10981 gaggatcacc cctttgacgc ccagcgcgtt cagttcactg atggcagttg cagcatcggc
    11041 gcgcagagta tcctgcaatg caatgatacc cagcacgtca ttgttacgta ctaccagcac
    11101 taccgtttgc ccggcgcttt ccagttcgtt aattaaacca gcaaatgcaa cagccggatg
    11161 ttttccggca gcgcatatca acacgcgctc accgttaacc tgcgcttcaa tgccagaccc
    11221 aaccagcgcc cgctgtgatt cggcggcggg aatggcgagt tcagcaacct gtgcttcgcg
    11281 tacgatggct tgcgccagtg gatgcgtcgc gccttgctcg accgccgctg ccagtgtcag
    11341 cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg gtttaccgac
    11401 ggtcagcgta ccggttttat caaacgccac ctgggtaaca cgacctagtt gttccagcgc
    11461 cgcaccgcct ttaatcaacg ccccacgacg cgctgctgct gccagcccgg aggttatcgc
    11521 cgcaggcgtt gagataacta acgcacacgg gcaaccaatc agcagcagcg tcagcccttt
    11581 ataaatccac tcttgccagc tggcggcaaa cagcagcggc ggcaccagcg ttaccagcag
    11641 ggcgatggcc ataatcgctg gcgtgtaaat acggctgaaa cggtcgataa acctctcaat
    11701 gggggcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa tggcgctggc
    11761 acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg ctcctgcagg
    11821 aactttatcg cccgtcgcgc gctccaccgg aatggattct ccggtcaggg cgctttcatc
    11881 aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgcccgc ccgccgcaac
    11941 ttcaatcaca tcgccagggc gcaggctgtt aatcgccacc tcttcccgct caccgttacg
    12001 caggcgcgtg gcggtttctg gtttcagcgc catcaacgcg ctaacgccct gacgcgcgcg
    12061 gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca ccatcgcagc
    12121 ttcagccgtt gcgccaataa acagcgcacc aatagcagct acgctcatta acgtttcaat
    12181 ggcgaagtaa ctgccggatt tgatcagccg taatgcctga cgagcaatcg ggtacagccc
    12241 aaccagcgtg gtcgcgataa acgccagttg cccgaacgga tgattaaact gctccagacc
    12301 ccagctgatt gccatcatca cgattagcgt aatcagcggc aaattctctt tcaggcgtga
    12361 tgtttgcggt tcgtcggcgg cctgttcatc gcgcagggaa tagcccgctt tttgcaccgc
    12421 agattcaacc tgggcacgga tgtcattgtc ggcatcgacc accagttttt cggtggcgaa
    12481 caacacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta ccttgcgcgc
    12541 acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg agacgttttc
    12601 agagagcgtt ggcgtgctgg aacatgcgcc gtcgcagcaa cagttgttgg cgttctgtgc
    12661 cgtggttagc ggtttgaacg cagcaaagtg aggggctttc ttgccgtgat tgtcaggagt
    12721 cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac tctggagtcg
    12781 actccagagt caagttttat cagagataca gcgagcggac gatcaggaag tgaccggcaa
    12841 agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag tggctcccca
    12901 gccagacaaa gttactgata aatagcaacg aagcgccaac aaacgcagag agcgccggag
    12961 cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc gtcatgccga
    13021 taaacgtgca gataggccaa cggtactctt ccagccgcgt ccagataatc gccagtaaca
    13081 gcgcccccag aaccagcaac accagtggca gaggccagaa gaaagagagc gtcatctgac
    13141 tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg gcgtacatca
    13201 gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac agccccgcca
    13261 gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac agcaggagaa
    13321 gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac gatgcatcca
    13381 cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta gttccttgaa
    13441 ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag tggcgagggt
    13501 ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt ccgatgaggg
    13561 ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat catcgtcgcc
    13621 gcatcgtttc gttttatgca acaacgacgg gaaaaagctg ataatgatat ggcaccgctc
    13681 cagcaaaagt tggtggtggt gagcaacaag cgggaaaaac cgattaacga tcgccgttcg
    13741 cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag cttcaaaccg
    13801 caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca cgccctgaca
    13861 gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgtcagctt tgttggcgaa
    13921 caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc aaacacgccg
    13981 aaaaggaaaa tccgtacctt ttcggcggtg gtcatctgcg ggccatcttt cggcaaggtc
    14041 gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag cgccacgtta
    14101 acaaaaatat tcagcgggcg tgggaaggga tgcaccaggt tgaggattaa aaatccccaa
    14161 acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt gtgcttcgcg
    14221 ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg accagtttgc
    14281 cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg cttcgtcagc
    14341 cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac ggcggaacgg
    14401 cggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg acatcgcgtt
    14461 gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa ttaactgctg
    14521 agaaaccgcg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg ataacgcttc
    14581 cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa caatgaccgg
    14641 agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca gacctgggct
    14701 atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga tttggccgct
    14761 gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa cacatacttg
    14821 caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt tagctgctat
    14881 gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg caaatggcga
    14941 aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag cagaccccgg
    15001 aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt caggcgcaag
    15061 agcctgcgaa ggcttcggaa caagccgttg aagagcagcc gcaggcgcat actgaggccg
    15121 aagcggaaac ttttgctgcc gaagttgtgg aagtcactga acaggttgct gaaagtgaaa
    15181 aagcgcagcc tgaagcggaa gtcgttgcac agccggaact ggtcgcagaa gaaacgccgg
    15241 agccagtggc tatcgaacgt gaagagctgc cgttgccgga agacgtcaac gccgaagagg
    15301 tttcgccaga agagtggcgg gctgaagcgg aaaccgtaga gattgtcgaa gcggcggaag
    15361 aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg ctggctgccg
    15421 aagcggcaga agaggcagtg atggtggttc ctccggcaga agaagagcag ccggtagaag
    15481 aaatcgctca ggagcaggaa aaaccgacca aagaaggttt cttcgcgcgc ctgaaacgca
    15541 gcctgttaaa aactaaagaa aatctcggtt ccggatttat cagcctgttc cgcggtaaaa
    15601 aaatcgacga tgatctgttt gaagagctgg aagaacagct gttgatcgcc gatgtgggcg
    15661 tggaaaccac acgtaaaata atcaccaatc tgacggaagg cgcatcccgc aagcagcttc
    15721 gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt ctggcgaaag
    15781 tcgatgagcc gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg gtaggcgtca
    15841 acggtgtggg taaaaccacg acgattggta agctggcgcg tcagtttgag cagcagggta
    15901 aatcggtgat gctggcggcg ggcgatactt tccgtgcggc agcggttgaa cagcttcagg
    15961 tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg gattccgcct
    16021 ctgttatctt cgacgccatt caggccgcta aagcgcgtaa tatcgacgtc ctgattgccg
    16081 ataccgccgg acgcctgcag aacaaatcgc acctgatgga agagttgaag aaaatcgtcc
    16141 gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact atcgatgcca
    16201 gcaccgggca gaacgcggta agccaggcca aactgttcca tgaagccgtt ggcttaaccg
    16261 gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc tcggtggctg
    16321 accagtttgg tattcctatc cgctacattg gtgtcggcga acgtattgag gatttgcgtc
    16381 cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa caatgattcg
    16441 ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg gcgttacgtt
    16501 ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag ggaaaagtac
    16561 cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct ggtttagcgg
    16621 ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc agattggcat
    16681 gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg tggcgatccc
    16741 gctgattatc gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg cggcactgga
    16801 taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttcgg gcggtgaaca
    16861 acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc tggcggacga
    16921 accgactggt aacctggacg acgcgttgtc ggaaggcatt ttacgtctgt ttgaagagtt
    16981 taaccgcgtt ggggtaaccg tattgatggc aacgcacgac atcaacctga tttcgcggcg
    17041 ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg gccatgaata
    17101 agcgcgatgc aatcaatcat attcggcagt ttggcgggcg tctggatcgc ttccgtaaat
    17161 cggtcggcgg ctcaggcgac ggcggtcgta acgcgccaaa acgcgcgaaa tcctcgccaa
    17221 aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc ttccacggcg
    17281 cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg gttatcgcca
    17341 tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaac caggcggcga
    17401 cgcaatatta tccgtcaccg caaatcactg tttatctgca aaaaacgctg gacgatgacg
    17461 ccgctgcggg tgtggtggca cagttgcagg ccgagcaagg cgtggagaaa gtgaactatc
    17521 tttctcgtga agacgcgctg ggcgagttcc gtaactggtc tggttttggt ggtgcgctgg
    17581 atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa ctcgattttc
    17641 aggggacaga atcactgaat acgctgcgtg atcgtatcac ccagatcaac ggcattgacg
    17701 aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttaaccggg ctggtcgggc
    17761 gcgtttccgc gatgatcggc gtgttgatgg tggcagcagt gtttctcgtc atcggcaaca
    17821 gtgtgcgtct gagcatcttt gctcgccgtg actccattaa cgtgcagaaa ctgattggtg
    17881 cgacggatgg tttcatcctg cgcccgttcc tgtacggtgg cgcactgctg ggattttctg
    17941 gcgcattgct gtcattaatt ttgtcagaaa ttctggtact gcgtttgtca tcggcggttg
    18001 cggaagtggc acaggttttc ggaacaaagt ttgatatcaa tggcttatcg ttcgatgaat
    18061 gcctgctgtt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg cttgccacgg
    18121 tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc tttccctgca
    18181 atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc atctctatgt
    18241 cacattttgt gcgtaattta ttcacagcgt tgcattgaac ttgtggataa aatcactgtc
    18301 tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt gttgctacca
    18361 ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat gcaaagttta
    18421 gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc gtggccgatg
    18481 ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg cgatctggaa
    18541 gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc tcgtaattat
    18601 gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg cctgatgaaa
    18661 gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc cgtccactgg
    18721 atcaaagcag agatccacga atacgttctg cgtaactggc gtatcgtcaa agttgcgacc
    18781 accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg tctgggctgg
    18841 tttaaccagg atgaagttga aatggtggcc cgtgaactgg gcgtaaccag caaagacgta
    18901 cgtgagatgg agtcgcgtat ggcggcacag gacatgacct ttgacctgtc ttccgacgac
    18961 gattcggata gccagccgat ggctccggtg ctctatctgc aggataaatc atctaacttt
    19021 gctgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct gaccgacgcg
    19081 atgcaaggtc tggacgagcg tagccaggat atcatccgcg cgcgctggct ggacgaagac
    19141 aacaagtcca cgttgcagga gctggctgac cgttacggtg tttccgctga gcgtgtgcgc
    19201 cagctggaaa agaacgcgat gaaaaaactt cgcgctgcca ttgaagcgta atttccgcta
    19261 ctaagcagag aaccctggat gagaatccgg ggtttttgtt ttttgggcct ctgcaataat
    19321 cagtttcccc tccggcaaaa cgccaatccc cacgcagatt gttaataaac tgtcaaaata
    19381 gctattccaa tatcataaaa atggggtatg ttttagcaga gtatgctgct aaagcacggg
    19441 tagtcatgca taaaacgaaa taaagtgctg aatcacaaca ccacaacaca cgtaataacc
    19501 agaagaatgg ggaacctcag gatgaacatg aagggtaaag cgttactggc aggatgtatc
    19561 gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt cgtgggcgca
    19621 atgtccggtc cggtggccca gtacggtgac caggagttta ctggtgctga gcaggcggtt
    19681 gcggatatca atgctaaagg tggtatcaaa ggcaacaaac tgcaaatcgt gaaatatgac
    19741 gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa cgacggcatt
    19801 aaatatgtga ttggtcacct ctgttcttca tcaacgcagc cagcgtcaga catctatgaa
    19861 gatgaaggca ttttaatgat caccccagcg gcaaccgcgc cagagctgac cgctcgtggc
    19921 tatcagctga tcctgcgcac taccggcctg gactccgacc aggggccgac ggcggcgaaa
    19981 tatattcttg agaaagtgaa accgcagcgt attgctatcg tccacgacaa acagcagtac
    20041 ggtgaaggtc tggcgcgtgc ggtgcaggac ggcctgaaga aaggcaatgc taacgtggtg
    20101 ttctttgatg gtatcaccgc cggggaaaaa gatttctcta cgctggtggc gcgtctgaaa
    20161 aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg gcaaatcctg
    20221 cgtcaggcac gcgcggcggg gctgaaaact cagtttatgg ggccggaagg cgtggctaac
    20281 gtctcgctgt ctaacattgc gggcgaatca gcggaagggc tgctggtgac caagccgaag
    20341 aactacgatc aggttccggc gaacaaaccc attgttgacg cgatcaaagc gaagaaacag
    20401 gacccaagcg gcgcgttcgt ctggaccacc tacgccgcgc tgcaatcttt gcaggcgggt
    20461 ctgaatcagt ctgacgatcc ggctgaaatc gccaaatacc tgaaagcgaa ctccgtggat
    20521 acggtgatgg ggccgctgac ctgggatgag aaaggcgatc tgaaaggctt tgagttcggc
    20581 gtatttgact ggcacgccaa cggcacggcg accgatgcga agtaatcatt aatcggcaac
    20641 tttgggttgc cgccaaattg ctaatatcga gtacgttgct tcatgccgga tgcggcgtaa
    20701 acgccttatc cggcctacaa gatccaaaga aatcagtaaa ttgcaacaca cgttgtaggc
    20761 ctgataagcg tagcgcatta ggcaatttct cgttggtcat cacttttgaa atcaaacttt
    20821 acgattaacg cttctcccag ccgccctgtt gtgccgtaaa tcccagtgcc tgcataaacg
    20881 ctgtcatcac gccgcgatct tccacgcctg catccgccat ccaccagcat gaaacgccag
    20941 gattgttacg caaaacctct tccagcagat attgccccac accgcgacgg cgggtgactt
    21001 ccctaacacg cagggaatcc agtgccccct cggtgccgct taaggttacc cgcacggccg
    21061 cgagaaggcg ctcgttaaaa cgcgcggcgt agatacggtg gttatcgtca acctgtaaag
    21121 aggaagggga atactccggc cagatctt
//
LOCUS       DARNVM010000076        21147 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.076, whole genome shotgun sequence.
ACCESSION   DARNVM010000076 DARNVM010000000
VERSION     DARNVM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.076"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1397
                     /gene="caiT"
                     /locus_tag="SI621_002573"
     CDS             <1..1397
                     /gene="caiT"
                     /locus_tag="SI621_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HEI4065277.1"
                     /translation="NAVFSYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPP
                     EFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHW
                     GPLPWATYSFLSVAFAYFFFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVAL
                     IFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIA
                     SDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGW
                     TVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLM
                     DKNIINIPNLIEQYGVARAIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAM
                     STCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMV
                     TLSFIKDAKQNWKD"
     gene            1428..2570
                     /gene="caiA"
                     /locus_tag="SI621_002574"
     CDS             1428..2570
                     /gene="caiA"
                     /locus_tag="SI621_002574"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HEI4065278.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2699..3916
                     /gene="caiB"
                     /locus_tag="SI621_002575"
     CDS             2699..3916
                     /gene="caiB"
                     /locus_tag="SI621_002575"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HEI4065279.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTSEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGKIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            3990..5543
                     /gene="caiC"
                     /locus_tag="SI621_002576"
     CDS             3990..5543
                     /gene="caiC"
                     /locus_tag="SI621_002576"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HEI4065280.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYALGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLIDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGTTFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5652..6437
                     /gene="caiD"
                     /locus_tag="SI621_002577"
     CDS             5652..6437
                     /gene="caiD"
                     /locus_tag="SI621_002577"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HEI4065281.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMSAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6443..7033
                     /gene="caiE"
                     /locus_tag="SI621_002578"
     CDS             6443..7033
                     /gene="caiE"
                     /locus_tag="SI621_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HEI4065282.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLIGTPARAVRNVSDEELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7152..7547)
                     /gene="caiF"
                     /locus_tag="SI621_002579"
     CDS             complement(7152..7547)
                     /gene="caiF"
                     /locus_tag="SI621_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HEI4065283.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7808..11026)
                     /gene="carB"
                     /locus_tag="SI621_002580"
     CDS             complement(7808..11026)
                     /gene="carB"
                     /locus_tag="SI621_002580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEI4065284.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDREKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKTLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11044..12192)
                     /gene="carA"
                     /locus_tag="SI621_002581"
     CDS             complement(11044..12192)
                     /gene="carA"
                     /locus_tag="SI621_002581"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEI4065285.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAETYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12648..13469)
                     /gene="dapB"
                     /locus_tag="SI621_002582"
     CDS             complement(12648..13469)
                     /gene="dapB"
                     /locus_tag="SI621_002582"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEI4065286.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13636..14550)
                     /gene="rihC"
                     /locus_tag="SI621_002583"
     CDS             complement(13636..14550)
                     /gene="rihC"
                     /locus_tag="SI621_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HEI4065287.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFASELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14616..15566)
                     /gene="ispH"
                     /locus_tag="SI621_002584"
                     /gene_synonym="lytB"
     CDS             complement(14616..15566)
                     /gene="ispH"
                     /locus_tag="SI621_002584"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEI4065288.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15568..16017)
                     /gene="fkpB"
                     /locus_tag="SI621_002585"
     CDS             complement(15568..16017)
                     /gene="fkpB"
                     /locus_tag="SI621_002585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEI4065289.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16105..16599)
                     /gene="lspA"
                     /locus_tag="SI621_002586"
     CDS             complement(16105..16599)
                     /gene="lspA"
                     /locus_tag="SI621_002586"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEI4065290.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16599..19415)
                     /gene="ileS"
                     /locus_tag="SI621_002587"
     CDS             complement(16599..19415)
                     /gene="ileS"
                     /locus_tag="SI621_002587"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEI4065291.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKELLGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLFAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19458..20399)
                     /gene="ribF"
                     /locus_tag="SI621_002588"
     CDS             complement(19458..20399)
                     /gene="ribF"
                     /locus_tag="SI621_002588"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEI4065292.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20407..20625)
                     /locus_tag="SI621_002589"
     CDS             complement(20407..20625)
                     /locus_tag="SI621_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HEI4065293.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20728..20991
                     /gene="rpsT"
                     /locus_tag="SI621_002590"
     CDS             20728..20991
                     /gene="rpsT"
                     /locus_tag="SI621_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEI4065294.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         4961 a         5735 c         5487 g         4964 t
ORIGIN      
        1 ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg
       61 tagtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag
      121 gtaacgaacc acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta
      181 cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc
      241 cgtttggctt agaaccgaac tcgacagggg cgaaagagtt ggggctggct tacagcttgt
      301 tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact
      361 tcttctttgt ccgcaaaatg gaagtgattc gccccagctc gacactagtg ccgctggtag
      421 gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct
      481 tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc
      541 aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga
      601 ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg
      661 acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca
      721 gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca
      781 tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct
      841 actgggcatg gtgggtgatt tacgctatcc agatgagtat cttcctcgcc cgcatctccc
      901 gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgat ggggctgaca gcgtcaacct
      961 ggatcctgtg gaccgtgctc ggtagtaaca ctctgctgtt gatggataaa aacatcatca
     1021 acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa acctgggccg
     1081 ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg
     1141 ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg
     1201 atggtgaaga accaccgctg ctggtgcgta tcggctggtc aattctggtt ggcattatcg
     1261 gtattgtttt gctggcgctc ggcggcctga aaccgattca aactgccatt atcgccggag
     1321 gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac
     1381 agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt
     1441 taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact
     1501 gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag
     1561 cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg
     1621 cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct
     1681 atgtgctgta ccagttgccg ggcgggttca acaccttcct gcgcgaaggc acacaagagc
     1741 agatcgacaa aattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta
     1801 ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg
     1861 gtaagattta tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca
     1921 tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg
     1981 ttgatatgag caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg
     2041 atagctgctg tgaaatcact tttgacgacg tggaactgga cgagaaagac atgttcggtc
     2101 gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag
     2161 ccctgaccaa ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc
     2221 agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc
     2281 acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag
     2341 acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg
     2401 catttgaagt ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc
     2461 accgcatcag ccgcttctgg cgtgatctgc gtgtagaccg cgtctccggg ggatctgacg
     2521 aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc
     2581 tgcctgatgc gacgctgacg cgtcttatca gacctacggg agcacgaatg taggccggat
     2641 aaagcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
     2701 ggatcatcta cccatgccga aattcgggcc gctggccgga ttgcgcgttg tcttctccgg
     2761 tatcgaaatc gccgggccgt tcgccgggca aatgttcgca gaatggggcg cggaagttat
     2821 ttggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
     2881 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc
     2941 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagcaaag gtccggcctt
     3001 tgcccgtcgt ggcattaccg atgaagtcct gtggcagcac aacccgaaac tggttatcgc
     3061 tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
     3121 caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
     3181 gcctgccttc ccgtataccg ccgattactt ttctggtctg accgccacca cggcggcgct
     3241 ggcagcactg cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta
     3301 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
     3361 gtgcccgcgc atgaccaaag gtaaagatcc ctactacgcc ggttgcggtc tatacaaatg
     3421 cgccgacggc tacatcgtga tggaactggt gggcattact caaattgaag agtgctttaa
     3481 agatattggc ctcgcacatc tgctcggtac gtcggaaatc ccggaaggca ctcagcttat
     3541 ccaccgtatc gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc
     3601 tgcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
     3661 agtactgacc gtaccggaac tggaaagcaa tccacagtat gtggctcgcg aatcaatcac
     3721 tcagtggcaa acgatggatg gtcgcacctg caaggggccg aatatcatgc cgaaattcaa
     3781 aaataacccc ggaaaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
     3841 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagta aaggtctggc
     3901 caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata
     3961 acaaacgaac aacaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc
     4021 aaatgtggga cgatctggcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca
     4081 gcagcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg
     4141 caaacctgtt ttatgcgctg gggattcgca aaggcgacaa ggttgctcta catctcgaca
     4201 actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc
     4261 caattaacgc ccgcctgttg cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt
     4321 gcctgctggt gaccagtgcg caattttatc ctatgtatca acagattcag caggaagatg
     4381 cctctcagtt gcggcacatt tgcctgatag atatggcact tcccgctgat gatggcgtga
     4441 gttcgtttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat
     4501 cgactgacga tacggcggaa attctcttca cctccggcac cacctcccga ccgaaagggg
     4561 tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg caatgtgcac
     4621 tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta
     4681 ctgcggcgat ggcggcgttt tctgccggga ccacctttgt gcttgtcgag aaatacagcg
     4741 cccgcgcctt ctggggacag gtgcagaagt accgcgccac cattaccgaa tgtattccga
     4801 tgatgattcg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa cactgcctgc
     4861 gggaagtgat gttttatctc aacctgtcgg agcaggaaaa agacgcgttt tgtgaacgct
     4921 tcagcgttcg cttgctgacg tcttatggaa tgacggaaac cattgtgggc attatcggcg
     4981 atcgccctgg cgataaacga cgctggccgt cgattggtcg ggcgggcttt tgctacgaag
     5041 cggagatccg cgacgatcac aatcgtcccc tcccggctgg tgagctcggt gaaatctgta
     5101 ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg
     5161 cgaaagtact ggaagccgat ggctggctac ataccggcga taccggatac cgcgacgaag
     5221 agggcttttt ttatttcgtc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg
     5281 tctcctgcgt ggagctggaa aatatcatcg ccacccatcc caaaattcag gatatcgtgg
     5341 ttgtgggtat taaagattcg atacgcgatg aagccatcaa agcatttgtg gtgctgaatg
     5401 aaggtgaaac attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat
     5461 ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa
     5521 taattagaaa gaatcttaaa taaatgaaac ggcagggaac taccctgcca gctaataatc
     5581 acgctctaaa ataatatgca ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat
     5641 ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta
     5701 cccttgatcg cccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag
     5761 tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag
     5821 agaaattctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg
     5881 ctgactttgg cccgggtggt tttgcgggat taaccgaaat tttcaatctc gacaaaccag
     5941 ttatcgccgc tgtgaacggc tacgcctttg gcggcggctt tgaactggca ctggcggcag
     6001 attttattgt ctgtgccgat aacgccagct tcgccctgcc ggaagccaaa ctgggcatcg
     6061 ttcctgacag cggcggcgtg ctgcgtctgc cgaagatcct gccgcctgcc atcgtcaatg
     6121 aaatggtgat gaccggcaga cgaatgagcg cagaagaggc gctgcgttgg ggggtagtca
     6181 accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggcg cagcagctgg
     6241 ttaacagcgc tccgctggcg attgcggcgc tgaaagagat ctaccgcacc accagcgaaa
     6301 tgccggtaga agaagcgtat cgctatattc gcagcggcgt gttgaaacac tatccgtcgg
     6361 ttctgcattc ggaggatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccag
     6421 tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggttgt
     6481 tcacccgacg gcgtttgtcc atcccagtgc agtcttgatt ggcgatgtga ttgtgggagc
     6541 cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca
     6601 agcgggagcc aatattcagg atggctgcat tatgcatggc tactgcgaca ctgacaccat
     6661 cgttggggaa aacggccata tcgggcacgg agcgatcctg catggttgtg tgattggtcg
     6721 cgatgcactg gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag
     6781 cattgttgcc gccatgagct ttgtcaaagc aggctttagc ggcgagaaac gccagttgtt
     6841 gataggtacg ccagcccgcg ctgtacgcaa tgttagtgac gaagagttac actggaaacg
     6901 gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tgcatgaaac
     6961 gcagccattg aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac
     7021 gccgaaacgg tgatcttgcc ggatgcggcg taaacgcctt atccgacctg gctggaaagc
     7081 aaccgtaggc cgtataagat gcgccagcgt cgcatccggc gaccaggtcg cacggtatca
     7141 acgaaacgac attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
     7201 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaatttt
     7261 tcccgactgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc
     7321 agtcgctgac actggcatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag
     7381 cttatttctg tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
     7441 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
     7501 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
     7561 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
     7621 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
     7681 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc
     7741 tcatcagaga accgtattag ttattcgatc aaattagcgg atgaaaaata tccgccatga
     7801 cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt tcagtcgcat
     7861 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
     7921 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcacga cggcctgagg
     7981 tggtgttgat gatgtaggta tattcgccat tcttgatacg gtcctgaatg tgcggacgac
     8041 cttcatgcac cttgttcacc agacgcggat taatgcctgc ttcgcccagc acaatcgccg
     8101 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
     8161 cgcgttcttt gtcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg
     8221 agttgctgcc cagctgcgct ttggcgaacg cttcagcgaa ggtgcggccc acgcccatga
     8281 cttccccggt agagcgcatt tctggcccta acagcgggtc aacgcccggg aatttattga
     8341 acggcagcac cacttctttc accgagtagt acggagggat aacttctttg gttacgccct
     8401 gctcggccag cgttttgcct gccatcacgc gcgccgccac tttcgccagc ggtacgccgg
     8461 tggctttgga gacgaacgga acggtacgcg ctgcacgcgg gttaacttca atcaggtaga
     8521 cttcgttgtt tttcaccgca aactgcacgt tcatcaggcc gcgtacctgc aattcgaagg
     8581 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
     8641 gcagcgaaca tgcggagtca ccggagtgca cgcccgcctg ctcaatatgc tccatgatgc
     8701 cgccaatcag caccatttcg ccgtcgcaga tagcatccac gtcaacttct actgcgtcat
     8761 caaggaagtg gtccagcaac actggcgcat cgttagatac gctgaccgct gtctggaagt
     8821 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
     8881 acggacgcac caccagcggg tagccaatct ctttcgcttt ctcaaccgcc atttcgatgg
     8941 tggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
     9001 gttcacggtc ttccgcacgg tcgatggcat ccgggctggt accgataacc ggtacgccag
     9061 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactgg acgataacgc
     9121 ctttcggctt ctcaatacgc acaatttcca gcacgtcttc cagagttacc ggctcgaagt
     9181 agaggcggtc ggaagtgtcg taatcagtgg agacggtttc cgggttacag ttaaccataa
     9241 tggtttcgta accgtcttcg cgcagcgcca gtgaggcgtg tacgcaacag tagtcgaatt
     9301 cgataccctg accgatacgg ttcgggccgc cgccgaggac catgattttt tcacggtcag
     9361 atgggttgga ttcgcactct tcttcataag tggagtacat ataagcggtg tcggtagcga
     9421 actctgccgc acaggtatcc acgcgcttgt aaaccgggtg caggtcatac tggtcacgca
     9481 gcttacggat ttctgcttca cgcacgcccg cgagttttgc cagacgcgcg tcggcaaagc
     9541 ctttgcgttt caactggcgc aggaagtcag cgttcaggcc agtgatgccc acttccgcca
     9601 ctttctcttc cagacgcacc agctcttcaa tctgtaccag gaaccagcgg tcaatgttgg
     9661 tcaggttgaa gacgccgtct acggacaggc ccgcgcggaa agcatcggcg atataccaaa
     9721 tacgctctgc gccagcgtct ttcagctcgc ggcggatttt agtcagcgct tccgggtcgt
     9781 cgagactcac tttcggatcg aagccggtcg cgcccacttc caggccgcgc agcgcttttt
     9841 gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat ttcatctgag
     9901 tggtcagacg gtcgttcgcc ccggcgaatt tctcgaagtt gaagcgagga attttggtga
     9961 ccacgtagtc gatggacggc tcgaaggagg ccggagtacg cccgccggtg atgtcgttca
    10021 tcagctcgtc gagggtgtaa cccaccgcca gtttcgccgc cactttggca atcgggaaac
    10081 cagtggcttt cgacgccagc gccgaagaac gcgacacgcg cgggttcatt tcgataacaa
    10141 tcaggcgacc gtttttcggg ttcaccgcaa actggacgtt ggaaccaccg gtttcaacgc
    10201 cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct ttgtcggtca
    10261 gcgtttgggc tggtgcgaca gtgatggagt caccggtgtg gatgcccatt gcatcgaagt
    10321 tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact tccatctcgt
    10381 actctttcca gccgatcagc gactcatcaa tcagcagttc tttggtcgga gagagatcca
    10441 gaccgcgggc gcaaatttct tcaaactctt cgcggttata agcgataccg ccaccgctac
    10501 cacccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg gcaaccgcca
    10561 gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca attttcttca
    10621 tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggca gtggcaccaa
    10681 tcatggtgac gccgaactct tccaacacgc cctgacgttc cagctccagc gcacagttca
    10741 gcgccgtctg accgcccatc gttggcagca ccgcgtccgg gcgctctttt tcaataatct
    10801 tgcgtaccac ttcccagtga atcggctcga tgtaggtcgc atcagccatt tccgggtcgg
    10861 tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct tcacgcaggg
    10921 ctttacacgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata acaatcgggc
    10981 ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct cttttactcc
    11041 tgattactta gcggttttac ggtactgctc aattaactcg ataaagtggt cgaacaacgg
    11101 cgctgcgtcg tgtggcccag ggctggcttc agggtgaccc tggaagctga acgccggttt
    11161 atcggtgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag
    11221 gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac
    11281 cacgtttttc tccacatctt taaccggatg gttgccgccg tggtgaccaa atttcatttt
    11341 gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgaggcaga tgccgaagac
    11401 cggaatatcg gtttcgagga atttctggat ggcggtaatg gcgtaatcgc acggggccgg
    11461 gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcaaca catcttccgc
    11521 agaagtttgc gccggaacga tggtcaggcg gcagcctcta tccaccaaca tgcgcaggat
    11581 gttgcgcttg gcaccaaaat cataagccac aacatggaac ggcagctcgt cttctttctt
    11641 cgcttctggc aggccgccgg tcagcgtcca gctcccttgt gtccagctat acgtttctgc
    11701 ggtggtcact tcttttgcca gatccatgcc gttcagacct gggaacgcgc gggctttttc
    11761 taacgccagc gccgcatccg ggttatcgcc cgcgataatg cagccattct gtgcgccttt
    11821 ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca cgatgttatg
    11881 gcgcttcagg taagaagaga ggtcttcggt attacggaag ttgctggcaa tcagcggcag
    11941 gtcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt
    12001 gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat aggaaggatc
    12061 agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc caaccgccga
    12121 acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc
    12181 tgacttaatc aaaacaccct ccagagaata ttcactcact ttatttgcat attaattcac
    12241 aatgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa acggcggcat
    12301 tctggagata tgggagctaa aagtcaactt aatggcggga ttttttatat tattttgtgg
    12361 ctcatgcatg ttttacaggc attaagcgac aaaaagatca tataacatgt tctggaaaac
    12421 gcttgcgcag caagaagatt gcatgaaatc aaaatgagca gaaaaaagtg gaccaaacgg
    12481 tcaaaattta cattaaaaca acaaaaacag aatacaaaac atgtagtttt cagcacttgc
    12541 attaaattta aaagcataac accaaaaata aaagggctaa aaaataaagc cctttatatt
    12601 caatgcatta aatgtgttat ttttgcatca ctataaatat tttgtggtta cagattattg
    12661 agatcaagca catctcgcat atcaaaaaga ccactttcct taccactcaa ccacaacgcc
    12721 gatcttaccg caccgttagc aaatgtcatg cggctggatg ccttatgggt gatctccaga
    12781 cgctcgccaa tatcggcaaa catcgcggta tgttcaccaa cgatgtcacc tgcacgcacg
    12841 gtggcaaaac caatggtgcc aggcacacgt tcaccggtat ggccttcacg actgtagacc
    12901 gcgcaatctt tcagatcttt atcaagagcg tgggcgatcg cctctcccat tgccagtgcg
    12961 gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc gatatcggtg
    13021 tagtccccca tcactttggc tgctttctcc agcagtttaa gcatgacgtt aacgccaacg
    13081 ctaaaattgg cggcaaagac aatcgcaata tcggcggctg cgtcacgaat cgcctgttta
    13141 ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg gcgacaaaaa
    13201 gcgagatgat tcagcgtacc ttccggacgg gtaaaatcga taaacacatc aaaatcatct
    13261 tttaccgcat cgaggctgct ttgcacggta acgcctgttt tccctgctcc agccagctca
    13321 ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc cagctgcacg
    13381 ccttctaatg ccagcgccgc ctgaatcaac tggcggccca tacgcccccc ggctcccgcg
    13441 atggcaacgc ggatgtttgc atcatgcata gctattctct tttgttaatt tgcatagacc
    13501 gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat aattagaaat
    13561 taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga ctgcatgccg
    13621 ataacatgtg acaggttacg gcaccagagc cagcacctca gctacccact gctggaagcc
    13681 ttttacattc agatccaatg ccacctgtac attggctggt ttgcccaggc aaccgtcgat
    13741 atcaaccacc gtcgtgccag aggtaaattc gccctgcgtt tccactgcca caaaacacgg
    13801 cttgagagtg aacaggtccg ggcgcaccag ccaggcgatg gcgcagagat cgtgcattcg
    13861 cagtccactt tgcatactgc cgctacggta gtgactaaac agggcgtgga gcatttttcc
    13921 ggtgcgattc aactccggca aagtagcgag atagtcaggg gttaatattg cctgattggt
    13981 gacatccaga ccgcacatga cgatttcgat gccgctacgg aagacacagg ctgccgcttc
    14041 tggatcggca gcgatattaa actcggcgtt tggtgtgcag ttgccgcgac cagcagaacc
    14101 gcccatgatc accagacgac gaatatacgg tttacattcc gggcattgtg aaagtaacag
    14161 cgcaatattg gttaacgggc cgatggccac cagggtaaca ggctcaggcg cacgcatcag
    14221 ggcatcacga atcgccagaa acgctggtat cccaagtggc tttcggttgt gctcaacaaa
    14281 gtcgtagcca gccattcccg attcgccgtg cacagatgcc gcatcacgcg gtgcgcgtac
    14341 cagcggcaca gcggctccct gagcgagcgg aatctccgca ttccagaaat gcagcagttg
    14401 cagggcgttg cgggtggttt tctcaaccga gacattaccc gcgacggtgg tcatcagttg
    14461 caggtcgagt tcggaggcaa aaatcgcggc agcaatggcg acggcatcgt caatgccggg
    14521 gtcagtatcg aggaagatag gtaaacgcat gttttctcca taaaaaatgc cggtaacaag
    14581 accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg aatatcgaca
    14641 cgcagctctt tcggcacttc gaaaacaatg ttttcttcac ggccttccag cggaatggct
    14701 tcaccaccgc ccagctgctg caaacgtgcc accacattct gcaccagaat atccggagcc
    14761 gatgcgcccg cagtcacgcc gacgcattta acctctttca cccactcttc ctggatatct
    14821 ttcgcatcgt caatcaaaaa cgcgcgtttg cccatccgct gagccagctc cgccagacgg
    14881 ttggagttag aggagttttt cgaaccgacc accagcacca cttccgcctg ctctgccagg
    14941 gcgcgtactg cttcctgacg gttagtagtg gcgtagcaga tgtcatcttt gcgcggaccg
    15001 acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtgtc atccaccgac
    15061 agcgtggttt gggtcataaa ggagagcttc tcttcgtttt tgaccgtcag tttccacaca
    15121 tcgtctggcg attcgaccag atacattccc ccttccgggt tgctgtactg acccatcgtc
    15181 ccttccactt ccgggtgccc ggcgtgaccg ataagaatag attcttcgcc acgacggctg
    15241 gcgcgggcga cttccatatg cactttggtc accagcggac aggtggcatc aaacaccgtc
    15301 aaatcgcggc tttttgcttc gttacgtacg gcctgagaaa caccgtgtgc ggagaaaatc
    15361 aggattgcgc cgtccggcac ttcgctaatc tgctcaataa agatagcccc acgctcacgc
    15421 aagctatcga ccacgtagcg gttatgcacc acttcgtgac ggacatatat cggtgcgccg
    15481 taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc acaaaaacca
    15541 cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga tttccagcac
    15601 ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga
    15661 gtcgccgtta atttcgcgga tcacgccagg catctcactg ccatccattg cggtaaaaag
    15721 catgattgcg ccaatttctg gctcgcctgc atccataaac tcacggcggg agaagtactg
    15781 aatcagatcc ggtgacggca cgccaaacgc cgcatctggc tccagcgaga aagtggtttt
    15841 atcgcccact ttcagcccca gcaggtgttg ctccagccct tcggaaagag aagcatcgcc
    15901 caggcggaat agcgccggtt taccgttgtt gcgggtagac tcggcggtgg tgccatcgtc
    15961 gagttttagc gtgaaatgca ccaggacggc gctattgctc tgtacagatt cagacatgca
    16021 ggtttgctct tatattttga ggatttttgc cggatgctgc ctacgatttc gtagcaatct
    16081 gtaggccgca tccggcaggg tttattattg ttttttcgct ttagaaggta aaaacccttc
    16141 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    16201 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    16261 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    16321 cgtggccttc gagcgataca tcatcactgc caggatcacg ctaataccaa tcgcaatacc
    16381 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    16441 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    16501 gttctggagg atcaggtact tgctgcccag atcgataatc agcacgacta ccaccagcca
    16561 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    16621 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgctaccttg
    16681 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    16741 gcgactttca gccctttgag tacttcgctc tgctgggcat cagcaggtgc gtcgttatag
    16801 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    16861 agtttcgccg ccagttccgg ttctgcgaac aaggtcactg ccgcttccag cgagccaccc
    16921 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    16981 agctcgtccc agaacgcatc gttcatcgct tcactgtctg ccagaccaaa caggccttcg
    17041 taccactcgc cggtgaagac gtatttttca cgttcacccg gcaggtagcc ccacacttca
    17101 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttccgc gatgtgatac
    17161 agcgctgtct ggcagctacg acgcgccaca ctgtccgctt tggcggtgta ctgacggtct
    17221 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    17281 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    17341 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    17401 gccggatcaa aaccgttcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    17461 tagctgtcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtca
    17521 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    17581 gaaacggtgt tgccgatgga tttagacatc ttgcggccct gaccatccac ggtaaagccg
    17641 tgggtcagta cctgacgata cggtgctttg cccttcatcg cggtagagat catcagagag
    17701 gacatgaacc agccacggtg ctggtctgaa ccttccagat acatgtccgc agcgtgaccg
    17761 gcaaattccg gacgcacgtc aacaacggaa gagtgggtag atccggagtc aaaccataca
    17821 tccagcgtat ccggcacttt cacgtactga tcagcttcgt cgccgaggag ctctttcgca
    17881 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    17941 agttcgaggg tacgcggatg cagctcttcc gtgtctttat gcacgaacag tgacatcggt
    18001 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    18061 atacgcgcct ggccccagtc cgggatccac tgcacgcctt tgatctcttt cagtgactgc
    18121 gcacgcagac ctttctgatc catgctgacg aaccactgcg gcgtcgcgcg gaagatgatc
    18181 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcaacagc
    18241 gcgccttttt cctgcagcag cgcaacaacg atgtcgttcg ctttgaaaac gttcacgcca
    18301 tccagcgtcg ggtaagtacc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    18361 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    18421 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    18481 cccataaacg gatgggtaaa gcgcagcagc tcaagctccg caccttttac cgtgccgaga
    18541 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    18601 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    18661 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    18721 ttaacgttgc ttacggcaaa ttttgctttc agcgcatcct gatcgaccgc ctggaaagca
    18781 acgtcgatgg acggagaagt tttgtcgtaa tactcaactt ccgcttccgc cagcgcagaa
    18841 cggcagtcaa cgcaccagtg taccggcttc gcgcctttgt gcaggtgacc gttgccgatg
    18901 attttgccca gcgcgcggat gatattggct tcagttttga agtccatggt caggtacggg
    18961 tgggaccagt cacccagcac gcccagacgg ataaagtctt tgcgctgacc gtcaacctgg
    19021 gtcgcagcgt attcgcggca cttggcgcgg aactcagcgg cggtgaattt ctcccccggc
    19081 ttaccgtatt cttgctctac tttcagttcg attggcagac catgacagtc ccagccaggc
    19141 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    19201 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    19261 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    19321 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    19381 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    19441 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    19501 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    19561 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    19621 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    19681 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    19741 ggggaaacct gacggcgcag cggtacattc gccgtcggga aacctatagt gcgccctaat
    19801 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    19861 agagccagat tgtcatccgc aagggcctga cgcacggcgg tgctgctgat acgcacccca
    19921 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    19981 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    20041 aattttacgc gcaaatgctt caccagaaga tcgctgatga aattttgcgc ggttaacgcc
    20101 gcgaaacgcc tgtcgaaacg tacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    20161 cgcagttttt cccgcagccg ggtcagccgt gccggggctt tatcggttgc aaacagttcc
    20221 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    20281 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    20341 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    20401 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    20461 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggcgc aaatcacggg
    20521 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    20581 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    20641 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    20701 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    20761 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    20821 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    20881 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    20941 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    21001 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    21061 atgcgtgaga aaacacactg ccggttgcca gatagtcaaa agttacaggt tcttgcagac
    21121 atgcagttac actctctgta ctcttcg
//
LOCUS       DARNVM010000077        20936 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.077, whole genome shotgun sequence.
ACCESSION   DARNVM010000077 DARNVM010000000
VERSION     DARNVM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.077"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            89..589
                     /gene="tssB"
                     /locus_tag="SI621_002591"
     CDS             89..589
                     /gene="tssB"
                     /locus_tag="SI621_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEI4065295.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            624..848
                     /locus_tag="SI621_002592"
     CDS             624..848
                     /locus_tag="SI621_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065296.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPYWRRVLH"
     gene            899..2374
                     /gene="tssC"
                     /locus_tag="SI621_002593"
     CDS             899..2374
                     /gene="tssC"
                     /locus_tag="SI621_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEI4065297.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2381..2794
                     /gene="tssE"
                     /locus_tag="SI621_002594"
     CDS             2381..2794
                     /gene="tssE"
                     /locus_tag="SI621_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEI4065298.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIAQCIERYEPRISRAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2798..4648
                     /gene="tssF"
                     /locus_tag="SI621_002595"
     CDS             2798..4648
                     /gene="tssF"
                     /locus_tag="SI621_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEI4065299.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4612..5694
                     /gene="tssG"
                     /locus_tag="SI621_002596"
     CDS             4612..5694
                     /gene="tssG"
                     /locus_tag="SI621_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEI4065300.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAQGEKGTPELSLRDDPAQETLRFSADASLAFPLSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5719..6999
                     /gene="tagH"
                     /locus_tag="SI621_002597"
     CDS             5719..6999
                     /gene="tagH"
                     /locus_tag="SI621_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEI4065301.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDFPVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQIYDRVLREKQREPEA"
     gene            6996..7520
                     /gene="tssJ"
                     /locus_tag="SI621_002598"
     CDS             6996..7520
                     /gene="tssJ"
                     /locus_tag="SI621_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEI4065302.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7523..8854
                     /gene="tssK"
                     /locus_tag="SI621_002599"
     CDS             7523..8854
                     /gene="tssK"
                     /locus_tag="SI621_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEI4065303.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8859..9620
                     /gene="icmH"
                     /locus_tag="SI621_002600"
     CDS             8859..9620
                     /gene="icmH"
                     /locus_tag="SI621_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEI4065304.1"
                     /translation="MDEGSLSLPPFTGHHEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9629..12484
                     /locus_tag="SI621_002601"
     CDS             9629..12484
                     /locus_tag="SI621_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEI4065305.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKVAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQALVREIIALRQQLLGVAEDD
                     AASLPDADAAEDTPPESESESESESESESESESESESESESESESESESEQDNTGAVP
                     ADEADREQPEETAETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGV
                     PLNRLSQNEMSVITDLPKLLGDTIKGQDLAIASLHKHLLTARADLRLPGRPLGAFLLA
                     GPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVL
                     TEAIRQKPYSVVLLDEVEKAHPDVLNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNL
                     GYQVIVEHADDPETMQEELYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLAR
                     LDNVLRSRFGAEVVIEPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMA
                     ANTAIARIRLSAVDDAFTADVEDAQNDESVTKDETVL"
     gene            12481..13224
                     /gene="tagO"
                     /locus_tag="SI621_002602"
     CDS             12481..13224
                     /gene="tagO"
                     /locus_tag="SI621_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HEI4065306.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTT
                     PPDATATSQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     LEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13229..14641
                     /gene="tssA"
                     /locus_tag="SI621_002603"
     CDS             13229..14641
                     /gene="tssA"
                     /locus_tag="SI621_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEI4065307.1"
                     /translation="MAMDLRDPNVWITHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14750..18184
                     /gene="tssM"
                     /locus_tag="SI621_002604"
     CDS             14750..18184
                     /gene="tssM"
                     /locus_tag="SI621_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEI4065308.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQSLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLAEMGLYQGRR
                     IGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGAFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWAGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18195..19547
                     /locus_tag="SI621_002605"
     CDS             18195..19547
                     /locus_tag="SI621_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HEI4065309.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAEPDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQPKSNVENAVQTTVRTLIYAPETTASSTPEAVVPPLPDLPEMK
                     VEVRSLSENPPQASVMKQGSTVRGFITGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLGVSPVQPLETGMQMMRVADSRWPESLQQQQASTH
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMRREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDTRAQKQNIDALEKQINERLEGVLIRWLLLKNNVMPEAATGTTAEK
                     "
     gene            19571..20053
                     /locus_tag="SI621_002606"
     CDS             19571..20053
                     /locus_tag="SI621_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEI4065310.1"
                     /translation="MANMSYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMVTCEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20097..>20936
                     /locus_tag="SI621_002607"
     CDS             20097..>20936
                     /locus_tag="SI621_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065311.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRRQNTHHVILLTLDVAKKILSSITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDGNGKIIFNFNTLKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACLSFGLPVILVGGAILLTGIVCTVVLNEIDAQYHLSEKLKDAIRDG
                     LKRQ"
BASE COUNT         4968 a         5672 c         5769 g         4527 t
ORIGIN      
        1 gccattaggg cggcattgcc gatatttttt tgcggtacaa atatgccgtg ttcatggcct
       61 gtacgagata acatgacgga tcacttttat gagcaaaaaa tttgaaggca gcgtcgcgcc
      121 tcgcgagcgt ataaatatat cctatgtccc gaagacggac ggccagacgg ctgaagtgga
      181 gcttccgctt aacatgctcg ttgtcggcga taccggaaat acgcaggaga catccccact
      241 ggatgaacgt caggcagttt ccgttaacaa gcacaatttc ggtgcggtga tggctgaagc
      301 agccattggc ctcaatttta ccgtccccgc cacgctgaag ggaagcacaa cggacgatga
      361 actgaatgtg gcgctgaata tcaagtcact ggatgatttt tcaccggaca gcgtggcccg
      421 gcaggttccg gaagttaata aattgctgga attgcgtgaa gcactgactg cgctgaaggg
      481 gccgatgggc aaccttccgg cattccgtac ccagctccag gcattgctgg aaaatgagga
      541 gtcccgcgag cagttgctca aagaaatcgg ccaagtgagt gggaaataac caccactatt
      601 tcattaaaca gggttatcca gacatggcgg cacacaaaaa tatctccggg aaacaccccc
      661 tctctgacga agacattttg caggcggttt accgggtatg tttcagcccg tccggtgaaa
      721 tcaaccggga tggcgtggat gcgctgatta caacactgga tcgccgtatc agtgatctat
      781 tggatgagat tatccatctt tgtgagtttc agcagagcgc gccgtactgg cggcgcgtac
      841 ttcactgacg gcgaaaaccc ggtttcagaa acacagctaa aacagaagga ttttcagtat
      901 gtctttacag gaagaggaac tcgtttccag tcatgccggg cagactgatc aggcatcgtc
      961 gctgcttgat caaatcatgg ctcagacccg tattcagccc ggttcagaag gttatgacgt
     1021 tgcccgccag ggtgtgacag cctttattgc cagcatcctg caatccactg catctgccga
     1081 accggtcaat aaactggccg tggacagcat gattgcagat attgatgaac gcatcagccg
     1141 ccagatggat gtcatcatcc atgcgcctgc ctttcagcag gtcgagtcgt tctggcgctc
     1201 actgaaaacc atggtggatc gcgttgattt ccgtgaaaac atcaaggtca acgtgcttca
     1261 tgtcaccaag caggaacttc tggaagactt tgagtttgcg ccagaaatta ttcagtccgg
     1321 attttataag cacgtttatt cgtccggttt cggacagttt ggcggtgaac ccatcgctgc
     1381 cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac atgaaactgc tgcaatacgt
     1441 cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc tccgtttccc cggagtttat
     1501 ggggctgaac tcgtggaccg aactgcctaa tatcaaagat ctttatgcca tctttgaagg
     1561 tccggcctac accaaatggc gcgccctgcg tgactcggaa gattcgcgct atttagggct
     1621 tacggcaccc cgcttcctgc ttcgccagcc ctattcacca acagataacc ctgttaagaa
     1681 ctttaactat tacgaagatg tcagccagaa ccacgaagat tatctgtggg gtaatacggc
     1741 ctggatgctg gcatgcaata tcgcagacag ttttgccaaa taccgctggt gtccaaacat
     1801 tatcggtccg caaagcggcg gcgcagtgaa agatctgccg gtgcatctgt tcgaaacgat
     1861 ggggcagatt caggccaaaa tcccaaccga ggtgctggtc accgatcgcc gcgaatttga
     1921 actggctgaa gagggtttta tcacccttac catgcgtaaa gactctgata atgcagcctt
     1981 tttctctgca aactcggtac aaaaaccgaa gcactttccg ggaaaagacg cagaaaccaa
     2041 ctataagctg ggtacgcagc ttccttatct cttcatcatc aatcggttag cgcactacat
     2101 caaggtgttg cagcgtgaac aactggggtc atggaaagaa aggagcgact tagaaagaga
     2161 gcttaatacc tggatccgtc agtatgttgc cgaccaggaa aacccgcctg cagacgtgcg
     2221 cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg gatgtagaag gcgaacccgg
     2281 ctggtaccag gttgcgctaa gcgtgaggcc tcattttaaa tttatggggg caaattttga
     2341 gctttccctg gttggccggt tagacaggga gtaacacacc atgataccgg atcgggaaag
     2401 aaccacaggt tctctttttg agcgtatgga agcctcttca gccagaaacc ggcagggagg
     2461 cagtattcat tcgctcaggc aatccattcg ccagaaccta cgaaacattc ttaatacccg
     2521 gtccggtagt tgccggggtg cgcctgagct gggcattgat gaaccggaag gggctgaaaa
     2581 cttccgcgaa tcaatgagtc gggccatcgc gcaatgtata gaacgctatg aaccccggat
     2641 ctcacgcgct gaagtgcagg cagtggtttc atccgcctcc agcccgctgg atatgacgtt
     2701 tcatattaca gcctgggtga catttaacga aacgcatgag gtgcttgaat ttgatatggc
     2761 accgaatggc agccagcatt accgggtgga ctgacaaatg gaatttgaag aacgctattt
     2821 ccgggaagaa ctcgattacc tgcgccagct tagcaagctg ctggcaacgg aaaaacccca
     2881 tctggcccgc ttcctggccg aaaaagatgc agatccggat attgaacgcc tgctggaagg
     2941 ggtggctttt cttaccggca atctccgcca gaaaattgag gatgaattcc cagaactgac
     3001 gcacgggctt attaagatgc tatggcctaa ttacctgcgt ccggttccgg caatgaccct
     3061 tattgaatat acgccggata tggataagtc ttctgtgccg gtgttaatcc cccgtaatga
     3121 gcagtttaca accaacgccg gggaaattag agtcgatgaa gtgctgccct ccgatgctaa
     3181 aaaggaggag ccgcctccct gtaccttcac cctctgccgg gatatctggc tgctgcccgt
     3241 tcgcctggag cagattgaaa accgcagtac gacccgtaat ggtgttatca acatcacctt
     3301 ttcggtcgca ccgggaacgg acttccgcac gctggatctg aacaaacttc gcttctggct
     3361 cggcaatgac gacaactata cccgtgacca gctttattta tggttctgtg aatacttgca
     3421 gggtgccgac ctgactgtgg gtgaacagca tattcgcctg cctgagttta tgctgaaagc
     3481 tgtcggtttt gagccacagg atgccatgct gccctggccg aaaaacgtcc acagcggcta
     3541 ccggatcctt caggagtatt tctgttaccc ggatgcgttt ctcttttttg atctttgtgg
     3601 ttgtccggcg ttgcctgacg gattgcaggc tgaatccttt accctgcaac tgcgtttttc
     3661 gcgcccattg cccgtggaca tccggttgcg ccgcgactcc ctgcgcctgt attgcgcacc
     3721 tgccattaat ttatttatcc accatgcaga agccatcacg ctggacaacc ggcgggcaga
     3781 ctatccgctg gttcccagcc gccattatcc ggaacattac gatgtctttt ccgttaacgg
     3841 cgtgataagc caggtgcagg atatgttcag gaaaaaagat ctggggcgtc ctgtttcgac
     3901 gcaggccgcg cgccagtggc ctgcctttga aagtttcagc catcagatgg aatacagccg
     3961 gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca tccctgttcc atcgcggctt
     4021 tgatcatacc cttgctttta tacatgctga tggcagttat ccgtcagacg aatcgctgct
     4081 cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac cgtgagcttc cgtcacaaat
     4141 tcgttccggc gatatcaccg gcacaaccgg taaaaatgcg gctgttgctt catttcgcaa
     4201 cattacccgc ccgacgcaac cgctctggcc ggtcattgat ggcagcctgc actggtccct
     4261 gctctccgcc atgaacctga attatctgtc attactggat acggacgcgc tgaagcaggt
     4321 catcgccaac tttgatcgcc acgcaatcca tcatccgcag acggcgcggc tgtcacaaca
     4381 aaagctggat gccattgagc gtctggagac tcgccctgtc gaccgcctgt ttacgggcat
     4441 tcccgtccgg gggctggcct ccacgctgta tctgcacccg gagccgtttg tctgtgaagg
     4501 ggaaatgtat ctgctcggta cggtgctttc gcattttctg tcgctgtacg ccagcgttaa
     4561 ctcattccac atgctgaccg ttgtgaacac agaaagccag gagacatgga aatggacgga
     4621 aagaaccggg cagcatcctc ttatctgagt ccgggaaatc ccccggcaga taaagagcag
     4681 aatgatccgc tggcacaggt ttttcataac gcctgctctt acaatttttt tgcgatggcg
     4741 gagctgttgc accgcctggc acagggtgaa aaggggacgc cggaattatc cctgcgcgac
     4801 gatccggcac aggaaaccct gcgtttcagc gccgatgcca gccttgcgtt tcccctgagt
     4861 gatatcagtg cgctgaaacg ggatacgtca ggggcattca ggatgaccac tacctttatg
     4921 gggttacagg ggagccagtc gcctctcccc ggttattacc tcgatcacct ggcctggaaa
     4981 gcggtacatg aacaaagtcc ggtaggtgat tttctggata tgttcagcca ccgcctgacc
     5041 cagtttgtct ggcatatctg gcgtaagtac cgctatcaca tcagttttcg taacggcggt
     5101 gtggatgcgt tctcgcagcg catgtactcc cttgttgggc tgggccaccg ccagcttcgc
     5161 gataagctgg cgattaatca cagcaaaatg ctggcgtatt ccgggatcct ggcaaatccg
     5221 ggacgatccc cggaaataat ctgcggtctg gtgtcccact gttttgattt gtcagacgtg
     5281 acgctgcaaa actggcaacg tcgcaaggtg gatattgaac cggatcagca aaatagcctg
     5341 gggagttatt cacttaaaaa tggcgaaaaa ctggcaggaa gatcggtact gggcaacttt
     5401 gttctgggta cacgggtgcc cgatctcagc ggaaaattcc agctcagtat taccagcctg
     5461 acccgaaaac agttcctctc ttttctgccg tccggcgaaa acttcctgcc actgacgatg
     5521 tttgtgtcct tcatcctgcg cgatcagctt gcctgggact tacatctggg gctggcgccg
     5581 gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg cgctgctggg ctggaccagc
     5641 ttcctcggca caccggaaga acgaccttca gtcaccatca gggtacggtc gtaaattatg
     5701 ataatcacgg agcagaatat gccggaagag aaactacaga cgctttcatt gcaggtcatt
     5761 aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc tgttcacaca gcagggaaat
     5821 gtcggccatg gccccgaatg ccactggtcg gtacaggatc gtcagcagag cattccggct
     5881 caggctttta ccgttatcct gcacgatggc acattttgtc tgcgcccgca gacggcacaa
     5941 ctgtggctga accaggcaaa agtcacagca acatcagacc tgatacagct gcgccagggc
     6001 gatgagatcc agatagggcg tctgatggtg agggttcatc tgaaccgggg agatatcccc
     6061 cattacgatg aggaaatggc cactcccgaa accatcgtca ccaatcgcga tatgctcacg
     6121 gataccctgc tgtcaacgga gggtgcgcca cactatccgg gaatgactca ccggcatcag
     6181 cttgcagaca ccgtggtaaa cggtttttct accgatccac tccaggcact tcagtccgaa
     6241 agcctgatta ccacgggcga tccgctttca ggcattgcgg ctgtccggcc atcggcaccg
     6301 ctgtccgatc cggcaagtaa tggtgggatc aatactccgt ttatggatct accgcccatt
     6361 tatgccagcc ccggcgatcg taatgatgac ttccctgtgg cagaaatggc gcaacgccac
     6421 cttgcggtca cccccttact gcgcggtctc ggcggctcgc ttaccgtgag caattctgac
     6481 gatgcggatg attttctgga agaggccgga cgcacgttac aggccgcaat aaaaggtctg
     6541 ctcgatttgc agcaacagcg taacagcctc tcagacaaac atttgcgccc gctggaagat
     6601 aacccgctgc gcctgaacat ggattacgcc accgcgctcg acgtgatgtt tgccgaaggt
     6661 aaaagtccgg tacatctggc ggctcccgcc gctgtcagtg aaagcctgcg taatgtccgc
     6721 caccacgaag aagctaaccg ggcagcgatt gtggagtcac ttcgtgtcct gcttgacgct
     6781 ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc gccgcagcca tgaactgcgc
     6841 cagccgctgg atgatgccgg agcatggcaa atgtacagcc attattacga agaactggcc
     6901 tccgatcgcc agcaggggtt tgagatgctg tttaacgagg tctacgccca gatctatgac
     6961 cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa caggaaggcg tttctggcct
     7021 gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc gaaaaaaatc agcgaggtga
     7081 tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca accttcggaa ataaccgtca
     7141 ccctgctgac cgaaccggac accaatacca atgcggaggg tgaatcagcc gccgtggatg
     7201 tgcaactggt gtacctgact gacgactcaa aattgctggc agccgactat gaccagatag
     7261 ccagtacgcc gctaccggat gtgttgggta aaaactatat cgaccaccag gatttcaacc
     7321 tgttgccgga caccatcaaa accctgccgc cggtaaagct ggatgagaaa acgcagttta
     7381 ttggtgtcgt ggcctatttc tccgatgacc aggccactga atggaaacaa atcgagacgg
     7441 tggaaggaac cggccaccat taccgcctgc tggtgcatgt acgtcaaagc agcatcgaaa
     7501 tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac aaggtgatgt ggcaggaagg
     7561 gatgctgatg cgcccacacc atttccagca acagcagcgt tacaacgact acctggataa
     7621 ccagcgtttc cgggccatga atgatttatc ctggggattt accgaactca ccctcaacaa
     7681 tgaactgctg gcgcagggta agatcatgat tgacagcgcg tcaggcacac tgcccgacgg
     7741 caccgtcttt tctatccccg accaggacgc actgcccgat ccgctgcacc cgcaacattt
     7801 tccggacgaa agaagccgca atatttacct cgctctgccg gtcgccagtg atgtgagaaa
     7861 tgaaatcagc gacgggcggc gaatcgggcg ttaccggctg aattatgccg atgtccggga
     7921 tctgcattca gaagaaggtg acgcgcgaac actgacgctg ggtcaactga cgccgcgcat
     7981 tatgagcggt gcagaagata tgagcgccta tattacgctc ccgctttgcc gtatcagcga
     8041 tcgccatgcc gacggctccc tgacgctgga tgacgatttt atcccctcct gccagaatat
     8101 tcaggtcagt aagaaactgc gtgtttatct caaagaggta cagggggcca ttggcggacg
     8161 ggcaagcgat ctggcaaacc gcattggctc tccggcgcag agcggcatcg cggatgtggc
     8221 ggaatttatg atgttgcagt tacttaaccg taaccagacc cggtttaccc atcgcgctcg
     8281 tcgatcccag ctccacccgg aagatttcta ccttgatctt gccgggttgc tgggtgaact
     8341 gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg atcatcatga
     8401 cctgaccaaa acatttaaaa cgctgttacc ggaagtcaaa cgggcgctgc ataccgtact
     8461 gtcgccaaga gccgttaatc tgccgctgca tctgcgtgac ggtatctggc aggccgatat
     8521 ccatgacaca gaactgctgc aatctgccac ctttgtgctg gccgtggcgg caaacatgcc
     8581 ggtcgatcag atccagcgcc agtttatcca gcagtcgaaa atttcctcgc cggaaaaaat
     8641 ccgcaatatg gtcagtgtgc agatacccgg cattccattg cgtgccctga tggtggctcc
     8701 ccgccagctt ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga gcggccaggc
     8761 ctggacagaa atggctgccg ccggggccgt tgcactgcat gtatccggca gtttcccgga
     8821 tctgaacatg caactgtggg cgataagagg gtaagacgat ggatgagggg tcactgtctc
     8881 tgccaccatt taccggacac cacgaaaaaa gccagcgcga ctatcatctc gcgcttcgtg
     8941 gcaacagcct gaatccgatg atcgacgcag ccacgccgct cctcggtatg gtgatgcgcc
     9001 tttcaaccat gaacagtcag acgatgccgg agcatctttt tgctcaggtg gtgacagatg
     9061 ttcaggccgt ggagcaactg cttcaggagc agggctacga accgggcgtg attatctcgt
     9121 ttcgctacat cctctgcacg tttattgatg aagcggcgct cggtaacggc tggtcgaaca
     9181 agaacgagtg gatcaagcag tcattgctgg tccatttcca caacgaagcc tggggtggag
     9241 agaaagtttt catcctgctg gagcgcctga tccgtgaacc caaacgttac caggatctgc
     9301 tggaatttct ctggatctgt ttttctctgg gatttcgcgg gcgctacaaa gtggcagcac
     9361 aggatcaggg ggaatttgag cagatctacc gccgtctgta ccacgttctg cataaactgc
     9421 gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa aacccagggc gggcgttatc
     9481 agctcatcag tcgcctgact gttaaacata ttttttgtgg tggcgttgtc gtgctggcgt
     9541 tgttttacct gttctatctg ttgcggctgg acagccagac gcaggacatc ctgcaccagc
     9601 taaacaaatt acttcgataa ggacgtttat gatccagatt gatcttccca cgctggtaaa
     9661 acggctgaac ctgttctccc gccaggcgct ggagatggcc gcctctgaat gtatgagtca
     9721 gcaggcagcg gaaattaccg tcagccatgt gcttattcag atgctcgcca tgccacgcag
     9781 cgacctgcgg gttattaccc gccagggcga tattggcatg gaagagttgc gccaggcgct
     9841 gacggtggag aactacacaa ctgcccgttc tgcggacagc tacccggcat tttccccgat
     9901 gctggttgag tggctgaaag agggctggct gctggcgtcg gctgagatgc agcacagcga
     9961 attgcgtggc ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt atataccgcc
    10021 tgctgccgcc cggttgttga ccggcattaa ccgtgaccgt ctgcaacagg actttgtaca
    10081 gtggacacag gagtcggcgg aatcagtcgt gccggatgca gacggtaaag tcgcaggcac
    10141 actgacggac gctgctgaca ccctgcttgc ccgctacgcc aaaaacatga ccgcagacgc
    10201 ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc tgatgatcga
    10261 cattctctgc cgccgccgca agaacaaccc ggtggtggtg ggcgaagcag gcgtgggtaa
    10321 aagtgcctta atcgaagggc tggcgctgcg catcgtggca ggccaggtgc cggacaaact
    10381 gaaaaacacc gatatcatga cccttgacct aggcgcattg caggccgggg cgtcggtgaa
    10441 gggcgaattc gaaaaacgct tcaaggggct gatggcggag gtcatttcct ctccggtgcc
    10501 ggtcattctg tttattgacg aagcacatac cctgattggc gcgggcaacc agcagggcgg
    10561 gctggatatc tccaacctgc tcaaaccggc gctggcgcgc ggcgaactga aaaccatcgc
    10621 cgccaccacc tggagcgaat acaaaaaata cttcgagaaa gatgccgccc tgtcgcgccg
    10681 cttccagctg gtgaaggtca gcgaacccaa cgccgccgaa gccaccatta ttctgcgtgg
    10741 tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc
    10801 cgcagcaacg ctgagcgaac gttatctctc cgggcgtcag ttgccggaca aagccattga
    10861 tgtgctggat accgcctgcg cccgcgtcgc cattaacctt tcctcgccgc cgaagcaaat
    10921 ctcggcgctg accactctga gccaccagca ggaggcggaa attcgccagc ttgagcgcga
    10981 gcttcgcatc gggctgcgta ccaacacctc acggatgacc gaggtgctgg tgcagtacga
    11041 tgaaacgctg acggcgctgg atgaactgga agcggcctgg catcagcagc aggcgctggt
    11101 ccgggagatt atcgcgctgc gccagcagtt gctgggcgtg gcagaagacg atgcggcgtc
    11161 gttgccggac gcagatgccg cggaggatac gccgccagag tcagagtcag agtcagagtc
    11221 agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagtcag agtcagagtc
    11281 agagtcagag tcagagtcag aacaggataa taccggtgcc gtaccggctg atgaggccga
    11341 cagagaacag ccggaagaga ccgctgaaac agtttccccg gtacagcggc tggcccagct
    11401 cactgccgaa ctggacgccc tgcataacga ccggttgctg gtctccccgc acgtcgataa
    11461 aaaacagatt gcggcggtga ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca
    11521 gaatgaaatg tcggtcatca ccgacctgcc gaaattgctg ggcgacacca tcaaaggcca
    11581 agacctggcg attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtct
    11641 tccgggacgc ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga
    11701 aaccgtcctg caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa
    11761 tatgtccgaa ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta
    11821 cgttggctac ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt
    11881 agtactgctc gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccagtc
    11941 gttcgataag ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt
    12001 cttcttcctg acgtccaacc tcggctacca ggtaatagtc gagcacgccg atgacccgga
    12061 aaccatgcag gaagaactgt atccggtgct ggccgacttc ttcaaacctg ccctgctggc
    12121 gcgtatggaa gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc
    12181 cggaaaactg gcccgtctgg ataacgtgct gcgcagtcgc tttggtgcag aagtggtcat
    12241 tgaaccggaa gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag
    12301 gatgctggaa tcggtcatcg atggcgacat gctaccgccg ctctcgctgc tgctgttgca
    12361 gaaaatggcg gctaacacgg cgattgcccg gattcggttg tcggcggtgg acgacgcatt
    12421 tacggcagac gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt
    12481 atgagcaggt tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc
    12541 ttacccgcca ctgccgcgcc cgcagaaaca tcgtcaatgg ccggtgtggc ggtcgccgtc
    12601 gccaccacga ccccgccgga tgcgacagcc acgtcacagg ccatgcagtc ctgtcggcgg
    12661 gagtccgcgg cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg
    12721 tcagggtttg acggggcgct ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc
    12781 acagagctgg aaaaacgccg cgagggcaac accacagaac tgctggtgac gcaggtgccg
    12841 ggggagcgcc cgacggtggt gataaccacc ccggctatcg gccatgttcc gccgcgcccg
    12901 gtgctgatgt tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct
    12961 ctggatgtcc atgatatcgc cgtcactctg aatgccgaca gccgggcatt gcgtagccac
    13021 tggtttgtgc gcgagaacgg caccctgctg gagtccagcc gggggctgtc cgggattgat
    13081 gaaattaagc aactgttcgg cgcaaaaacg ctgacagtgg ataccggcgc agataacgcc
    13141 gcaggaaagc tgacctttaa cattgacggg ctggcaaggg cgattgcccc cctgcgcgtt
    13201 gcctgccact gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg
    13261 gataacgcac ctgctggaaa acctgccgga agaaaaactg gcatcggcgc tgaaagatga
    13321 caacccggac tgggagtata tcgacggcga aatcgtcaaa ctgggatctc tggcccacag
    13381 tcaactggat atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa
    13441 ggactttcgt ctgctggcgc atctgctgag aaccctgcaa cacgccggtg atcctcatct
    13501 cgcattgcgc ctgctggcgc tgtatgtgga gcattactgg tcggtggctg cgccgcagaa
    13561 tatggcacat aaaaaacgct ttgccagcca ggtcattaaa cgcttcgaaa caggtattga
    13621 aggcttttca cagaacgccg ccacagcgca gcgcgacgcg ctgtcagggg aactggcgaa
    13681 actggcgcag tgctggcagt cacataacgc cccggaactg gcactggcta ccgatgatct
    13741 ttttgccctg taccagcgtg cgtttaatcg tgcggcaccg gctcctgccc ccactccagc
    13801 ggcctccgat agttcacgac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag
    13861 tgctccggct ccccaaatcg ccatcgacag tcacgacgac aaagcctggc gcgacacgct
    13921 gttaaaagtg gcggctattt tatgtgaacg ccagccggac tcgccgcagg gctatcgcct
    13981 gcgccgccat gccctgtggc aatccatcac cagtacaccc caggcggaaa gcgatggacg
    14041 taccccattg gctgcggtct ctgccgatat ggtggcggat taccagtccc ggcttgccag
    14101 cgcggatatg gcgctgtggc agcaggttga gaaaagcgta ttgctggctc cttactggct
    14161 ggacggtcac tgtctttctg cacagacggc actgcgtctg ggttacaaac aggtggcaga
    14221 caccatccgc gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct
    14281 gtttaatgac cgcacaccgt ttctcagtga gcagacgaaa caatggctgg cagcttcgcc
    14341 cgacggcaaa gttgcaccgg ttgcgcaaat cggtgaggaa tcgcaggcag ccagagcctg
    14401 ttttgctggg cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga
    14461 tccccgcgat cagtttcacc gccagtacct tgccgcacag ttgacggagg aggcggggct
    14521 gatacagctt gcgcagcaac agtaccggat gttgttgatg atagggagtc agatgatggt
    14581 gtctgactgg gagccatcat tacttacgca gcttgaacaa aaattcacgg cagaacaata
    14641 aaagagaaca ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa
    14701 atcagcccta cgcccggcga tgccccggtt taaagtctcc gctttctggc tgctgatact
    14761 ggcgtggatt tttctgctgg tatggatctg gtggaaaggc ccgacgtgga cgctgtatga
    14821 agaacagtgg ctcaaaccac tggcgaaccg ctggctggca acggcggcat gggggattat
    14881 cgccctagtg tggcttaccg tccgggtgat gaagcgcctg caacagcttg aaaaaatgca
    14941 aaagcagcag cgcgaggagg ccattgatcc gctcagtgtg gagctgaacg cccagcagcg
    15001 ttaccttgac cgctggctgc tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg
    15061 gcagttgccg tggtatatgg tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg
    15121 cgagggtttt ccgtccgaca ttatttatgc cccggagggc gcacgcggcg cagaacaacg
    15181 cctgtatctc acgccccatg tcggtaaaca ggcggtgatc tttgatatcg acggcacact
    15241 ctgcgctccc gctgatgcgg atatcctgca tcgccgtctg tgggaacatg ccctcggctg
    15301 gctgaaagaa aagcgcgcgc gccagtcgct gaacgggatt attctgacac tcgatttacc
    15361 cgatctgctt accgcagaca aacgccgccg cgaacatctg ttacagacgc tgcgcagccg
    15421 cttgcaggat atacgccagc atcttcactg ccagttaccg gtttacgtgg tactcacccg
    15481 gcttgattta ttgcagggtt ttgccgccct gttccagtca ctgaacagac aggatcgcga
    15541 tgcaattcta ggggtcacgt tcacccgccg cgcccatgaa aatgatgact ggcgaacaga
    15601 gttgaatgct ttctggcaga catgggtgga tcgaatgaac ctagcgttgc cggatctgat
    15661 ggtcgctcag actcacaccc gcgcgtcttt atttagtttt tcccgccaga tgcagggaag
    15721 ccgtgaacca ctggtgtcac tgcttgaggg tctgcttgac ggcgaaaata tgaacgtgat
    15781 gctgcgtggc gtctatctca cctcttcgct tcagcgcgga cagatggatg atattttcac
    15841 ccagtctgcc gcccgccaat accggctggg caataaccca ctggcgtcct ggcctctggt
    15901 ggacaccgca ccctatttca cccgtagcct gttcccgcag gcgttactcg cagagcctaa
    15961 tctggcaacg gagagccgcg cctggctgat gcgttcccgt cgccgcctga cggttttctc
    16021 tgccactggc ggcgtggcgg cactgctgct catcaccggc tggcatcact attacaatgg
    16081 taactatcag tccggcatca ccgtgcttaa gcaggccaaa gcctttatgg atgtgccgcc
    16141 tccgcagggg gaagatgact atggcaacct gcaactgccg ctgctgaacc cggtacgcga
    16201 tgccactctg gcctacggtg actggggcga ccgcagccgc cttgcggaga tgggattata
    16261 tcaggggcga cgcatcgggc cttatgtgga acggacctac ctgcaactgc tggagcaacg
    16321 ctatcttccg tcgctgttta acgggctggt caaagagatg aacgccgcgc caccggagag
    16381 tgaggaaaaa ctcgccgtgc tgcgcgtgat gcgaatgctg gaggataaga gcggacgtaa
    16441 caatgaggtg gtgaagcagt atatggcaaa acgctggagc gaaaagttcc acggccagcg
    16501 cgatatccag gcgcaactga tgtcccatct tgactacgcg ctggctcaca cagactggca
    16561 tgcagagcgt caggcgggcg acggtgacgc tatcagccgc tggacgccgt atgacaagcc
    16621 cgttgtgtca gcacagaaag aactaagcaa actacctgtc taccagcgag tttaccagag
    16681 cctgaaaacg cgggcgctgg gcgttcttcc tgccgacctc aatctgcgtg accaggtagg
    16741 gccaaccttt gaccaggtgt ttacatctgc cgatgacaat aaactggttg ttccacagtt
    16801 tcttacccgt tacggcctgc aaagctattt tgtaaaacag cgcgatgaac tggttgaact
    16861 gacggcgatg gattcctggg tactcaacct cacccgcagc gtgaaataca gtgacgccga
    16921 ccgggcggaa atccagcgcc agttgaccga gcagtatatc agcgactaca ccgccacctg
    16981 gcgggctggg atggacaatc tgaatatccg caattttgag tccatcggac aactgaccgg
    17041 ggcgctggag caggttatca gcggtgacca gcctttgcag cgggcgctga ccgtgctgcg
    17101 tgacaacaca caaccaggcg ccttttctga aaagctatct gctaaagaac gggatgaggc
    17161 cctggctgaa ccagactacc agttactcac ccgccttggg catgagttcg ccccggaaaa
    17221 cagtaccctg acagtccaga aagataaaga aagcacgatg caggccgtgt acctgcagct
    17281 caccgagtta caccgctacc tgctggcgat ccagaacgcg cctgtacctg ggaaatcggc
    17341 actgaaagcc gtgcagttac ggcttgatca gaacagcagc gatcccatat tcgccacccg
    17401 ccagatggca aaaacactgc ctgctccgct caaccgctgg gcaggcaggc tggctgacca
    17461 ggcctggcat gtggtgatgg tggaagccgt tcactatatg gaagtggact ggcgcgacag
    17521 cgtggtgaaa ccgtttaacg agcaactggc aaataactat ccgtttaatc cacattctgc
    17581 acaggatgcc tcactggatg ccttcgaacg attctttaaa ccagatggca tactggatac
    17641 cttctaccag cagaacctaa agctgtttat cgacaatgac ctgagtctgg aggatggcga
    17701 taacaacgtc attattcgcg aagatattat tgcgcaactg gaaaccgcgc agaaaatccg
    17761 tgacatattc ttcagcaaac agaacggtct gggaacatcc tttgccgtgg aaacggtatc
    17821 gctttccggc aacaaacgcc gcagcgtact gaaccttgac ggtcagttag tcgattacag
    17881 ccagggccgt aactataccg cccatctggt ctggcctaac aatatgcgcg aaggcaacga
    17941 aagcaaactg acgctggttg gtgcccgcgg cggcgcaccg cgcagtatca gcttcagcgg
    18001 gccgtgggct cagttccgcc tgttcggggc cggacagctg accggagtac aggatggcaa
    18061 ctttaccgtg cgctttagtg tggacggtgg cgcaatgacc taccgcgtgc ataccgacac
    18121 ggaagataac ccgttcagcg gtgggttatt cagccagttt ggcctgtcag acacactgta
    18181 ctgaggaggc agggatgaat agtaacggac tgacacaaac tatcgttacc ggcagtgacc
    18241 cgcgcgggct gccggaattc agcgccatcc gcgaggaaat aaacaaagcc agccatccgt
    18301 cacagcctga gctgaactgg aaactggtgg agtcgctggc gctggcgatt tttaaagcca
    18361 acggtgtgga tttacacacc gccacctact atacgcttgc ccggacacgg acacagggac
    18421 tggcgggatt ctgcgaaggt gcggaactgc tggcggcaat ggtaagccac gactgggata
    18481 aattctggcc gcagggcggt ccggcgcgta ctgaaatgct ggactggttt aactcccgaa
    18541 ccggcaatat tctgcgtcag caaatctcct ttgcggaacc cgatctgccg ctgatctacc
    18601 gcacagagcg ggcattgcag cttatctgcg acaagcttca gcaggtggag ctgaagcgcg
    18661 ttccgcgcgt ggagaatctg ctctatttta tgcagaacac gcgtaagcgg cttgaacccc
    18721 agccgaagag taacgttgaa aacgccgtac agaccacggt tagaacactg atttatgccc
    18781 cggaaacaac ggcttcctcc acgccagaag cggtagtgcc tcccctgccc gacctgcctg
    18841 agatgaaagt agaagtgcgc agtctgtcag agaatccccc acaggccagt gtgatgaagc
    18901 aaggcagtac ggtaagaggg tttatcacgg ggatcgcctg ttcagtggct gtcgcctcag
    18961 cattgtggtg gtggcaggtc tatccggtgc aacagcaact gttacaggtt aacgacaccg
    19021 cgcagggcgc agcaacggtg tggatggcct cacctgaact agaaaactat gagcgcaggc
    19081 tgcaacagct tcttggcgtc tccccggtac aaccgctgga aaccgggatg cagatgatgc
    19141 gtgttgccga cagtcgctgg ccggaaagcc tgcagcagca gcaggcctcg acacattgga
    19201 atgaagcact caaaacccgc gcacagagta gcccgcagtt gcgtggctgg ttgcagaccc
    19261 gtcaggactt acatgctttt gcagatctcg tgatgcggcg cgagaaagag ggactaaccc
    19321 tttcctatat caaaaatgtc atctggcagg cggagcgggg actggggcag gaaacacccg
    19381 ttgagtctct gttgacgcag taccaggata cccgtgcgca gaagcagaat atagatgcgc
    19441 tggaaaaaca aattaatgag cgactcgaag gcgtgttaat ccgctggctg ctgctgaaga
    19501 ataacgtcat gccagaggcg gcaaccggga ccacggccga aaaataatta tttaattaaa
    19561 ggagattatc atggcaaata tgagttattt atctttgagt ggagagacac aagggctgat
    19621 ttcagcaggt tgctcaacgc ttgattctgt cggaaacaaa gcacagccag aacataaaga
    19681 tcagatcatg gtttatgcgt taatgcattc catctcaaga agtcaaaatg tcaatcatca
    19741 tgaattaatt atcaccaaac cggttgataa atcctcacca ttgctggcaa aagcacttag
    19801 tgacaatgaa aaaatggtaa catgtgaatt tatactctac cgaaccagta aagcgggtat
    19861 ttaccagcct tattataaaa taaacctgtc aaaagcccga atatcaagta ttgattttgt
    19921 cacaccgcat gctgtcctgg aaaaagaact ggagccacag gagagaattg cctttattta
    19981 tgaagatatt tcctgggagc atacgttagc cggtacgaat gcaatgagta aatggcagga
    20041 tcgtgtacag taatttactg taataacata ataaattact attgcggaga gatataatgg
    20101 ctggtttaag gaataataac aacacacaga atgcacagtg ggctgattat gtgggtgaca
    20161 ttcttcgggg ggctcaaccg ataaaccagc tagtacctca gcacccttat cttaacgacg
    20221 tcccactcat tgatgaatta aggcgtcaga acactcatca tgttattctc ttgacacttg
    20281 atgttgcaaa aaaaatactc agctcaataa catcatttga ttacattcat tttatcacta
    20341 cacatccttc aggtataaaa gatacactcg cttggttagt taacgcgggt aaattaatga
    20401 ctgagtttga tggaaatggg aaaattatat ttaactttaa taccttaaag tatacaaaag
    20461 caagttactt tgaaatactt ggagagaaat atataaaaat tacaacatcc agcccctggc
    20521 ttttagaaaa gctcggtaaa tatattttct cttcacgagc gcctcaagtt ctggaactcg
    20581 ctattggctg gcgaggagct ttatctgaat caataaaagg agttaagttc tgtatctggt
    20641 tttcagtagc ctggagaacc atagaattta ttatgagttc agagcgagat ctggttaatt
    20701 ttcttggtga tttttcaatg gatgtggcca aagcagtgat tgcaggcggt gttgcaactg
    20761 ctattggaag cctggcttct tttgcctgtc ttagttttgg ccttccagta attcttgtag
    20821 gaggagcaat tttacttaca gggatagtgt gtacagttgt tttaaatgaa atcgatgctc
    20881 aataccattt atcagaaaaa ttaaaagatg caattagaga tggactaaaa cggcaa
//
LOCUS       DARNVM010000078        20869 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.078, whole genome shotgun sequence.
ACCESSION   DARNVM010000078 DARNVM010000000
VERSION     DARNVM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.078"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(97..462)
                     /locus_tag="SI621_002608"
     CDS             complement(97..462)
                     /locus_tag="SI621_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEI4065312.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(754..1740)
                     /gene="yqjG"
                     /locus_tag="SI621_002609"
     CDS             complement(754..1740)
                     /gene="yqjG"
                     /locus_tag="SI621_002609"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HEI4065313.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPSALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVVKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1810..2202)
                     /locus_tag="SI621_002610"
     CDS             complement(1810..2202)
                     /locus_tag="SI621_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEI4065314.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2388..2687)
                     /locus_tag="SI621_002611"
     CDS             complement(2388..2687)
                     /locus_tag="SI621_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HEI4065315.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2677..3081)
                     /locus_tag="SI621_002612"
     CDS             complement(2677..3081)
                     /locus_tag="SI621_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEI4065316.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3084..3389)
                     /locus_tag="SI621_002613"
     CDS             complement(3084..3389)
                     /locus_tag="SI621_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEI4065317.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3427..3795)
                     /locus_tag="SI621_002614"
     CDS             complement(3427..3795)
                     /locus_tag="SI621_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HEI4065318.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLTEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3942..4325)
                     /gene="mzrA"
                     /locus_tag="SI621_002615"
     CDS             complement(3942..4325)
                     /gene="mzrA"
                     /locus_tag="SI621_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HEI4065319.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4329..4991)
                     /locus_tag="SI621_002616"
     CDS             complement(4329..4991)
                     /locus_tag="SI621_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEI4065320.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5336..6112)
                     /gene="exuR"
                     /locus_tag="SI621_002617"
     CDS             complement(5336..6112)
                     /gene="exuR"
                     /locus_tag="SI621_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HEI4065321.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6242..7540)
                     /gene="exuT"
                     /locus_tag="SI621_002618"
     CDS             complement(6242..7540)
                     /gene="exuT"
                     /locus_tag="SI621_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HEI4065322.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8023..9435
                     /gene="uxaC"
                     /locus_tag="SI621_002619"
     CDS             8023..9435
                     /gene="uxaC"
                     /locus_tag="SI621_002619"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEI4065323.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9450..10937
                     /gene="uxaA"
                     /locus_tag="SI621_002620"
     CDS             9450..10937
                     /gene="uxaA"
                     /locus_tag="SI621_002620"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEI4065324.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11020..11571
                     /locus_tag="SI621_002621"
     CDS             11020..11571
                     /locus_tag="SI621_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HEI4065325.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(11575..12819)
                     /gene="sstT"
                     /locus_tag="SI621_002622"
     CDS             complement(11575..12819)
                     /gene="sstT"
                     /locus_tag="SI621_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEI4065326.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13435..14400)
                     /gene="alx"
                     /locus_tag="SI621_002623"
     CDS             complement(13435..14400)
                     /gene="alx"
                     /locus_tag="SI621_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HEI4065327.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14684..15670)
                     /locus_tag="SI621_002624"
     CDS             complement(14684..15670)
                     /locus_tag="SI621_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEI4065328.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15749..16441)
                     /locus_tag="SI621_002625"
     CDS             complement(15749..16441)
                     /locus_tag="SI621_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HEI4065329.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(16518..17021)
                     /locus_tag="SI621_002626"
     CDS             complement(16518..17021)
                     /locus_tag="SI621_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEI4065330.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEQQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17106..18242
                     /gene="rlmG"
                     /locus_tag="SI621_002627"
     CDS             17106..18242
                     /gene="rlmG"
                     /locus_tag="SI621_002627"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HEI4065331.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(18360..20378)
                     /gene="fadH"
                     /locus_tag="SI621_002628"
     CDS             complement(18360..20378)
                     /gene="fadH"
                     /locus_tag="SI621_002628"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEI4065332.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYPNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSKNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         5185 a         5513 c         5351 g         4820 t
ORIGIN      
        1 acactgaaat attttaaata aacattagta aattattcat tcatcaacat gatgataaaa
       61 gccggatgag tcagacatcc ggcgagtaga tttgttttaa ggttccagtt ttggatcagg
      121 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      181 gccgataaac gggattaaga atagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      241 gcggcggaac tgaactgccc accatggtaa aaacaccaga ataccgtaga ttgtcgtcag
      301 gatgccttcg ccaccggcac gttgccagcc taacatttta tccagtagcc ccagcacgaa
      361 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      421 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      481 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      541 ttcgttcaag gaattaataa agtgccgtga aggcgcgcta agactgatga caagcgcaaa
      601 atcgccagat gcgctacgct tatctggcct acgcggcatt gtaacatatt gaatataaac
      661 gttttgtagg ttggagaaga cgttcacgcc gcatccgaca taaataacac gcacttagct
      721 aataatcaga ggcaccccgt aaggtgcctt tttttaaccg aagcgaacat ctcgtccatg
      781 cggttcatcg agatcctgcc acggaccaat tgaaataatc cccgtagggt tgatggtctt
      841 atgactgcgg aagtaatgat tacggatgtg atcgaaattg acggtttcgg caatccccgg
      901 catctggtag atatctcgca ggaaaccata caggttcagg taatcgctga tgcggtgctt
      961 atcacacttg aagtgggtga catacaccgg atcaaaacgc accagcgtgg tccacaggcg
     1021 aatatcggct tcggttagct ggttgccggt caggtaacga tgctggccta aaatctgctc
     1081 cagtcgcgcc agcgattcaa acactttcac taccgcctcg tcgtaggctt gctggctggt
     1141 ggcaaaaccg gctttataca cgccattgtt aacggtgtca taaatccagc cgttaagttc
     1201 gtcaattttc gtttgcaggg ctgatgggta gtaatcaccc gctttcgcgc ccagcgcatc
     1261 aaacgcggta ttgaacatgc ggatgatttc tgctgattcg ttgctgacga tggtgtggtt
     1321 ctttttgtcc cacagcacgg gaacagtaac tcgtccgctg tagtgtggat cggcgtggag
     1381 ataaagctga tacagaaatt catgttgata gagcgtatct ccggttgcac ccggaaaact
     1441 gtcatcaaat gtccagccgt tttccagcat cagcgggttc actacggaaa cggaaataaa
     1501 cggttccagt cctttgagtt tacgcatgat cagcgtgcgg tgcgcccacg ggcaggcgag
     1561 tgaaacatag agatgataac gatctttctc tgcgataaaa ccgcctgtgc cagtggggcc
     1621 tggcgcgcca tcggcagtga gccagttacg aaatgcggaa gctgaacgtt gaaatttacc
     1681 gccggtagat ttggtatcgt accaggtgtc atgccagacg ccgtcaatca gttgacccat
     1741 atttctctcc tccaatagca aaaacgagga gcttcctcct cgttttttat cgttaagtat
     1801 agctcgtgct taccactttt tattcagcag gcggtcgatg ctatacgcgc ccggaccggt
     1861 aattgccagc agcaggaatc cgccagaaat tgtcaggttt ttcatgaaca tcagcgagtt
     1921 gacgccttca gcaaagttgc tgtgaaataa aaatgccgtc agcagcgtaa agcccgcagt
     1981 aaacagggct gtggtgcgag tcaggaaacc gaacaggatt gctagaccac caccaaactc
     2041 aagcagaatc accagcggca gcataaagcc tgggacaccc atcgcttcca tatattgttg
     2101 ggtacccgcg tagccagtaa tttttcccca gcctgcggta ataaacagaa tcggcattaa
     2161 aatgcgcgct accagtacac caacatcttc taattttttc atcatactct ccactgcgcc
     2221 actcaggctg ctgattgttt ttttgtgcaa gggcgcggta ttagcgtcgt tgctgtcgat
     2281 ggagagaatc ataaacgtgg tgaatgatga atgttagcaa ggaaaactgt caaaaatctt
     2341 caaaaaattt gagggataag gccggaatgg ctccggccag agggaagtta accgcgaagt
     2401 tgttgctgct tgagggtcgt tttcaccaga cgccaggcgc tccatacgcc aaaaccgcgt
     2461 ctggcccagc ggaccagcat attaggatgg cgaatcgtcc agatcgccat cacgctactg
     2521 cctaccagcg cccaggagcg cagacttagc agcatattcc agcgacgatc gtaagcgcct
     2581 gttgcctcca gccattcacg acgactggcg gaaagatcca gccgttgttg ctggatctgg
     2641 ctcagcagtt gcgccttacg tcgttcacgt tcgactttac tgctcacggg actcctcctc
     2701 gagcagctgc cgatcgtttg ccagctcatg gcgtgtatgg cgcagcaggg tggatttacg
     2761 cgatttacgt aacgtccaga taccgccaat cagtgccagt agcagcaaca ccacggtggt
     2821 ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc caaataatca gcaccatcag
     2881 gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc agcatcagta aaagttgaaa
     2941 gagattcgct ttttcctctt ccagctccac caccgccagc cgcagacgtg tttccaccat
     3001 ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga acgcttttac cgggcccttg
     3061 cgcgtgatga gtgtccgcca taattaacga cgcgacagca gaacgccgag cactacaccg
     3121 attgcagcgc caatgcccac gcccgtccac gggttttcac gcacatactc atcggcacgc
     3181 gccgccgcga cacgggtttg tttggcgatg gcatcaccgg tttcacccag gcgataacgg
     3241 ctctgtttca gtgcctgctc cgctttgcta cgaatcttac tcaactcttc tttcgacttc
     3301 tcgccagatg agctaagtac ctcttccagc gtatcggaaa gggatttcaa ctcagcacgc
     3361 agatgttccg tagtgtgttc tttcgacata gttttctcca ggtgagtaag tagtgactat
     3421 tgtgagttag tagtcgcgcg cttccagctt tttcagctct tcctgcgctt ctgccagttt
     3481 ccgttcgcgt ttggcaatct tatcggcatc gcctttttgc ttcgcctcgg ttaaatcttg
     3541 ctggcgttcc gccacctcat ctttctgctt tgcgattttc ttctgatgat cggcacgcag
     3601 ctggctatct gaacagttgg cccggacttc actcagggct ttattcagac cgtcaatacg
     3661 attctggttt tggtgttttt cggcatagct gatctcctta aggatatttt gctccttttc
     3721 ctgacacagg gtagtggcat aactaccggc actaagagca aagagagaaa cagctaaagc
     3781 gatgcggtat ttcattcttg aaccttccat tgtgattcgg acctattaca aaggaacgat
     3841 tgtccagaca acgaagcggt agtgttaacc accgcctcat taatcataga cagttacggt
     3901 gaaatcacca aagtcagtga tatgtttcgg ataacaggaa gttatccgaa gcgatgagag
     3961 ttatcccgca accgggtcag ccactgcata gcctgactat tattgtcctg ctgcgcgatg
     4021 atgtagccat ggggcaatgt tctgtcgagg accgcttttg cggcggcact ctggtcgctg
     4081 gagtcaaagg taattaacag agtgtcattt ttgggggtga tacttttgaa aggaatgccg
     4141 tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca tcgttgtccc ttgatgaacg
     4201 gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg accaggccag caacagtgtg
     4261 ccgaccagaa gtaaaacagc gccggaccag gctagctggc gaagcgacat gcgaggtatt
     4321 tgcatccctt acccccgatt tccatatttc tttttccata acacgaccag agaacctgcc
     4381 aggccaaaca ccagcagcac caccgggagc agcatcaggc atgacatcag ctggtcctcg
     4441 tactttaaaa ataccggcgt tttgccgagc atgtaaccca gagttgtcag gatcaatacc
     4501 cacagcagac cgctcatcca gttgaagaac tgaaagcgcg cgttattcag ccctgataat
     4561 ccggcaatcg tcggcagcag tgttctgaca aatgcaataa agcgaccaat taacagcgcc
     4621 gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat gataatgcgc gggtaaatga
     4681 gatagccagt tttgtacagt gcgggtattg cccaaccatc gcccctgaat atagctgacc
     4741 cagcaaccga ggctggcggc aacggtcagc agcagaatcg tttgcggata gcccatcgcg
     4801 cctttcgcaa tcaatacgcc gaccaacacc agtaaactgt cgcccggtaa aaaggccgcc
     4861 ggaagcaagc cgttttcaag gaacaaaatt acaaacaaga caaaatacaa catgccaatc
     4921 atcgacggat tggccagggt ttcaaaatcc tgcgcccaca gggcttgcag caattgggtc
     4981 aaaagttcca ttcattattc ctggtgaatc ggttaacgcc acgctggttt taaaagatgc
     5041 actaccgcaa tgttattgct gttttggtat gttttgcggg tttgatacat caacacagcc
     5101 tgaactattc cctgttaaat cgtgagataa gaaagcattc aaaagcataa attcaaattg
     5161 aatctaattg taacaaaggt caacgttgtt cgttatcaaa attacatgtc cgtagagtct
     5221 gattttgctg attgcagggg agggtggcga gaggatttac acaggctgac actttatact
     5281 tttcgcttac cgacgaagca aacaggcgca ttgcagcgcc tgtcggatgg aaatttcatt
     5341 tgctgccgct ggtggccgta tcgagatgca ctaccgggtt ttctgcgaac agatagcggt
     5401 cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa catgattttg gtgttttcca
     5461 gatgctgcca cattgccagc ttagcagcat gaggatcttt gcgaattaac gccttgagga
     5521 tttgatcgtg gtcatcacac cagttatcga cggtacgggc atcaatgtgt tcgtgcagtt
     5581 ttttccagta cgggttatga ctacgctggg tccacatttt ttccacgata gccgccaggg
     5641 cggagttctg cgtcgccagg gctacctgaa tgtggaactg caaatcccac tcggaatcac
     5701 ggaagcattg ttcgccgcgc gcctgctcct ggatggccat cagtttcatg atgtcctgtt
     5761 tcgttacctg agttgccgcg aattcggcaa tattactttc gatgagctgg cgagcctgaa
     5821 gcaactcaaa cggaccgtaa ttggcgaatt ccatattatt gtcagccgcc tgctgatggc
     5881 gcggctggtt ggaaaccaca tgaatacctg aacctttacg cacttcgaca tagccttcaa
     5941 cttccagcat gatgatggct tcacgaacca cggtgcggct gacgtttttt tcatcggcaa
     6001 taaagcgttc ggcaggcagt ttatcaccca ccagatagac gccctgttcg atgcgctctt
     6061 tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc agtgatttcc atatgcgctc
     6121 caggcatgat acggcagact atatttgtta taccactttt gcgagtttag ctctactcac
     6181 agtgatgaaa aagccgcctg agaaggcggc tttgaaggga aactaaaact tacacgaagc
     6241 cttaatgttg cggtgcagga tcgttatgtg tttcctgcgc cacctcaatc gccggtttgt
     6301 tctgcaacac cgtccagata accagtgcac ccagcaggtc gaatactgcc agaactgcga
     6361 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca aacagcgtac
     6421 ttgccagcca tgcggacatc ccggttaaac cgtttgccgt tgccacttcg ttacgaccaa
     6481 acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgagca aaaccaccga
     6541 tacacagcag cataatcgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     6601 tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     6661 accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     6721 gcatcggcat ccaggcaaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     6781 tgaacagcgg gatccacgcg ttaaaagtac cccatgccgg ttctgccagg aagcgcggca
     6841 gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgctg
     6901 tgtcaacctg gtgctgggct tcctgaccat taataatgta gtcgcgttct tcatcggtca
     6961 gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccattgcc cagataaagc
     7021 tcaacgcacc ggagatgata aatgccatct gccagctgtg cattacgatt gcccatacca
     7081 ccagcggcgg tgcaatcatc gcaccaatag aagaacctac gttaaagtaa cctaccgcga
     7141 tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc gggatcatcg
     7201 ctgcttccgc ggcaccgacc gcaccacgag caacagccag gccgccccag ctacctgcca
     7261 gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg
     7321 tacccagcac atccagtaca taacctgcta ccggttgcat gaccgtataa gcagcagaat
     7381 aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg
     7441 gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca
     7501 gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt
     7561 tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg
     7621 ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg
     7681 gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc
     7741 gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga
     7801 gccatcacaa atgtgggaat atttgtaggg acattacctg acgacagcga ggccagtact
     7861 ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta cgtttattga
     7921 tctatctcac gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg
     7981 caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact
     8041 gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac
     8101 cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt
     8161 tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg cgctatgcgt
     8221 accaacggtg tggctgagcg tctgtgtact ggtgatgcgt ctgaccgtga aaaatttgac
     8281 gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg gacgcacctc
     8341 gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac tgccgatgaa
     8401 atctggaacg aatgtaatga actgctggcg caggataact tctccgcgcg cggcatcatg
     8461 cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc tctggagcat
     8521 cacgcagaga tcgccaaaga cggctctttc accattaaag tgctgccgag ctggcgtcct
     8581 gataaagcct tcaacatcga acaggcgacc tttaacgact acatggcgaa gctgggcgaa
     8641 gtttccgata ccgacatccg ccgctttgct gatctgcaaa ctgccctgac caaacgtctg
     8701 gatcacttcg ccgctcacgg ctgtaaagtg tctgaccacg cgctggatgt ggtgatgttt
     8761 gctgaagcga acgaagcgga actggacagc attctggcgc gtcgtctggc tggcgaaacc
     8821 ctgagcgagc acgaagtggc acagttcaaa actgcggtgc tggtgttcct tggtgccgaa
     8881 tatgcacgtc gcggctgggt acagcagtac cacattggcg cactgcgtaa taacaacctg
     8941 cgtcagttta aactgctggg gccggatgtc ggctttgact ccatcaacga ccgtccgatg
     9001 gcggaagagc tgtctaagct gctgagcaag cagaacgaag aaaacctgct accgaaaacc
     9061 attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc
     9121 cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt taacgatcag
     9181 aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgtt gagccgcttt
     9241 gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc
     9301 cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcgcc ggcggatatc
     9361 aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc
     9421 attgaactga actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata
     9481 acgtcgcggt cgctttagca gatttggcgg aaggcacaga agtcagtgtc gacaaccaga
     9541 ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa
     9601 aaggggccaa cgtcatcaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg
     9661 cgggggaaca cgttcacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc
     9721 gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct
     9781 atcgtcgcgc taacggcgat gtcggggtgc gcaatgagct gtggatcctg ccaaccgtgg
     9841 gctgtgtcaa cggcatcgcg cggcagatcc aaaatcgttt cctgaaagag accaacaacg
     9901 ccgaaggtac cgacggcgtg ttcctcttca gccacaccta cggctgctca cagctgggcg
     9961 acgatcacat taatacccgc accatgctgc aaaacatggt gcgccacccg aacgcaggcg
    10021 cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgc
    10081 tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga
    10141 tcgaggccgg gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg
    10201 agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct gacggtcttt
    10261 ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt gctaacggcg
    10321 gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg ctgatggacc
    10381 attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaatgac ttcaagcagt
    10441 actttattgc ccatgaccag ccgatctatg aaaacccatc gccggggaac aaagcgggcg
    10501 gtatcaccac gctggaagac aaatcacttg gctgtaccca gaaagcgggt tccagcgtcg
    10561 tggttgacgt gctgcgttac ggtgagcgtc tgaaaacacc ggggctgaac ttgttaagtg
    10621 cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc catatggtgc
    10681 tgttcagtac tggtcgcggc acgccatacg gcggctttgt accgacggtg aaaatcgcca
    10741 ccaacagcga actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga
    10801 tccacggtaa agcgatgccg cagttgctgg aagaattcat cgataccatc gttgagtttg
    10861 ccaacggtaa gcaaacctgt aacgagcgta acgacttccg cgaactggcg atcttcaaaa
    10921 gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg
    10981 aagcgccgtt tgcttttcca gacctgctaa ggatttatca tgaccgcgta ttggctggcc
    11041 cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa ccgtgacgaa
    11101 cgccgcttta aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt
    11161 ctgggcacct atcccgccgg tgccagtgcc atccttaatg ccattcgtac gctgattact
    11221 ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc
    11281 ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtaa tcggcacgat tgtcagtacc
    11341 tgggcgctgt tccgctgtaa agggctgacc atgcgctgcg taatgtggtt ttcaacgtgt
    11401 tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat tgaggggagt
    11461 tttctgctca tgaatggcct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt
    11521 gatccgttta aagtcgagaa aaccccttcc gccgtggacg aaaggggtta aacattaatt
    11581 acgtagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag
    11641 cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa
    11701 gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag
    11761 tgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac acagagaagc
    11821 cactacgctc aacagcagcg ctgtgggcag atcgaccgga atacccagcg tattaaccgc
    11881 agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggcacccag
    11941 cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca gcgccatatt
    12001 caccggaatg ttcgccgcag agctgcgggt gaagaaggcg tacacgccgc tttcgcgcag
    12061 gcacagcagc accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt
    12121 aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc
    12181 ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccgat
    12241 cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac
    12301 caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac
    12361 caggatcccg atgtagttac ctttcagcag cgcatcgatg gggttggaaa ccatgctcat
    12421 taccagcccg cgcatcactt ccacaatacc agacggcggc gaaatatcac ccgcgctact
    12481 ggacaagtgc agggtagaag ggaaggcaaa actgaagatt actgcggcca gagcagcaga
    12541 gaaggtgccc agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg
    12601 gtggttagca atagatgcca tcaccagcat cagcaccaga attggcgcta cagctttcag
    12661 tgcgccgacg aacaaagtgc ctaacagacc aacagcttct gccgcgggtt ttgagatcca
    12721 tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc
    12781 cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt
    12841 tgtcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc
    12901 aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag
    12961 aggtcatatt ttgcaactta atggttacat ttatttgaca taaacagagg gaaggactgg
    13021 ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag aacggttgcg
    13081 ctcctttaat gtgctatgcc atataaagtc actggcgcag ggagcgcgca gggggcggcc
    13141 aatcgccgcc gccccctgca cccccaggct ctggcgaaca aaatcgccgc tgcgcggttc
    13201 cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct gtaagatgcg
    13261 ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg gcgattttga
    13321 cgccaccaac acccgtgcgg tctttttgta aagagctata agccgattac ataattaata
    13381 gctcaccttt gtctggaatg agttatctct aggaatggct taatatacag cgaatcattc
    13441 tccccgctgc ttatcatgcc gataattcac ccaggcgttg ataataaatg tcatcaccag
    13501 aatgccaaac accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag
    13561 catcttgata ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg
    13621 ttctgctacg cccgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt
    13681 tgaggtcagc acaataaacg gatcggtggt cacggcgaag atagccggga tgctatccac
    13741 ggcgaaaatc acgtcgctca attccaccag aatcagcacc agcatcagcg gcgtggcata
    13801 cagcaagcca ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa
    13861 atgaccgcgt agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc
    13921 gagcgccatc ttcacgccgg taaacaacag gaaggcaccg aagatataca ggatccagtc
    13981 gaactgcgaa atcagccagc tgccagtgaa gatcatgatg gtacgcagaa caatcgcccc
    14041 gagcacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa
    14101 caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacccgt
    14161 gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcggtttgca ccagatacca
    14221 ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc
    14281 ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag
    14341 cataatggcg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat
    14401 agaagttcct tacacataaa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    14461 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    14521 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
    14581 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt
    14641 caatggtctg ttacatgcct tttacactgc tttactttgg caattatagt tttacgctat
    14701 ctgccggaaa aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc
    14761 gactgaccgc cagcccagga tgatccacca gatgttcatc caccagttca gcaaacagct
    14821 ctgcttcgta gagcatggta ttaatatgct gcggttgggt gagatcctgc atctggctgc
    14881 cacgcggaac aaagcaaact ttctggcatt cagacagttt ttcaatcacc agcgatcctg
    14941 cttcgccctg aatctcgctt gccaggacag aatcgctcac ttttgagtgc tgcaacgtaa
    15001 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atccacaccg cttgccagca
    15061 aactggcggt tgcctgcacg cttttcggtt caccgaataa tgccaccgcc gacgccaggc
    15121 agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag gtgttgggat
    15181 tctcaccatc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    15241 tacgcagttt gccgactttc ggcagtgcct ggcgtaacag atgaaagttc ggcaggctgg
    15301 cggttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
    15361 ccacttccgc cagattcgat gccagcggct tctcgcaaat cacatgaatt ttgtggctaa
    15421 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
    15481 tggcatcgct ttctgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat
    15541 tggcgaagtg ctgggcctgc tcaaggctgc gggaataaac ggcggttaac ttgtatttac
    15601 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    15661 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
    15721 tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca tgctcactaa
    15781 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
    15841 caatcaccgc gctgacgcga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
    15901 tattgaggtc tggcgcgtta aaaccgatag cgtcgatgcc gtactgtttt gccagccaga
    15961 tggcgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttcc ccaaacacct
    16021 ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt
    16081 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgatgcttca tcgtaatttt
    16141 tacgtccgtt atcgccactg actaacagcc atttcacttt tccggcgtgg tacaatgctg
    16201 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctggctccc agcaataaac
    16261 ccacgttacg cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggtgttga
    16321 ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaat aaacagccaa
    16381 tcttaagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaag gcgcgcagca
    16441 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaagg aagaaaagat
    16501 aacgcctgac atcattttca gattttgtcc tgcccaagtg acaactgcgt tagccacaga
    16561 cgagtgtcga attcaagctg gtggtactgc tgctccatat gacagcacaa ttgatagaac
    16621 gctttgttat gctctttctc cttcaggtgc gccaactcgt gcaccacgat catgcgcaaa
    16681 aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctccgcctt tgctttcagc
    16741 ttgccgccct gcacacgcga aacagcggta tgtaacccga gcgcgttttt cagcacatgg
    16801 atcttgttgt catacatcac tttagtgatc ggcggggcat tgcgcagaaa ctgatttttc
    16861 agatcctggg tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggataa
    16921 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt
    16981 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    17041 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat catccaggag
    17101 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    17161 cggatgacgt taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt
    17221 tggacgacac agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    17281 gttgtgcgct ggcggaacat aagccataca gcattggcga ctcatacatc agcgaactgg
    17341 cgacgcgcga gaatttacgc ctcaacggga ttgatgaatc gagcgtgaag tttctcgaca
    17401 gcaccgctga ctacccgcaa cagccgggag tggtgctgat caaagtaccg aaaacactgg
    17461 cattgctgga acagcaactg cgtgcgctgc gcaaagtggt cacgccagat acgcgtatta
    17521 ttgccggtgc caaagcccgt gacatccaca cttccacgct ggaactgttc gaaaaagtgc
    17581 tcggcccgac caccaccacg ctggcatgga agaaggcacg cttaattaac tgtactttca
    17641 acgagccgcc gctggccgat gcgccgcaga ccgttagctg gaaactggaa ggtactgact
    17701 ggactatcca caaccatgcg aatgtcttct cccgcaccgg gctggatatt ggcgcgcgct
    17761 tctttatgca gcatctgcca gagaatctcg aaggtgagat tgtcgatctc ggttgtggta
    17821 atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg
    17881 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgcccgagg
    17941 cattggatcg ctgcgaattt atgatcaaca acgcgctctc cggcgttgag cctttccgct
    18001 ttaatgctgt gctctgcaac ccgccgtttc atcagcaaca tgcgctgacc gataacgtcg
    18061 cctgggagat gttccaccac gcccgccgct gtctgaaaat caacggcgag ctgtatatcg
    18121 ttgccaaccg tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca
    18181 ccatcgccac gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt
    18241 aagattggca gctacctgcc agataaggcg taaacgcctt atctggcata cgttcagcac
    18301 attaccccca ggtcggataa gacgcggcaa gcgtcgcatc cggcacttca atgcacgatt
    18361 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata
    18421 gctacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc
    18481 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc
    18541 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcga tcttctgata acttacgcct
    18601 gggatcattt tcacgccgcg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
    18661 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatctg ccgtgggcta
    18721 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgcaggct gctgtcgatc
    18781 ccccactcat tacagaactc ggcaatgttc ttgctggtgg actcacccgg ctgacttaaa
    18841 aacatcgccg tatcaaaacc aatcccgcca caaccgatga ttgctacctt tttgcccaca
    18901 ggcgctttgt cgcgcagtac gtcgagataa ctcaacacct tcggatgatc gattccgtcg
    18961 ataggtggaa tgcgcggtac gatcccgcta gcgaggatcg tttcatcgaa cgactgtaac
    19021 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ctgttacttc gatcatccgg
    19081 cggtaatagc gcagcgtttc gtaaaactcc tctttgccag ggatctgttt ggcgatatta
    19141 aactgaccgc cgatctcact gtgagcatca aacaacgtta cctgatgacc acgtgccgcc
    19201 gcgttaatgg caaacgccag cccagccgga ccagcgccga ccaccgccag attttttttc
    19261 tgcacggcgg gaaggatcgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
    19321 gaggtgactt tgcccacgaa gatctgatcg agacaggcct ggttgcagcc aatacaggtg
    19381 ttgatctcat cggctcgccc cgattgcgct tttgacagca gctccgcatc agcaagaaac
    19441 ggtcgtgcca tcgataccat atcggcatca ccgcgcgaga gaatgtcgtc ggcaacctgc
    19501 ggatcgttaa tccggttggt ggtcaccagc ggcagcgaga cgtggccttt cagtttgcgc
    19561 gtgacccagc taaaggcacc gcgcggcacg ggcgtggcaa tggtcggaat acgtgcttca
    19621 tgccagccaa tgccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagc
    19681 cctaccgttt cggcaaaagt accgccgcct tctaccaggt cgagcatcga cagacggtag
    19741 ataataatga agtcattgcc gacgcgttcg cgcactgctc gcaccacttc aactgcaaag
    19801 cgcatccggt tagggtaatc gccgccccac tggtcgctac gctggttagt gcgcagggtc
    19861 agaaactcgt tgatcaaata cccttcggaa cccatcactt ctacgccatc gtatccggcc
    19921 tcccgcgcca gttgcgcgca gcgggcgaaa ttgtcgatca gttgcaggat ctcttcatgg
    19981 cttaactcat ggggaacgaa acggttgatg ggagcctgca atgcggaagg agcgaccaga
    20041 tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc
    20101 tgatgtacgg cttcggttat ggtgcgatgg tgcgggatct ggcttgcgtc attgagcatt
    20161 gcgccgcctt ccatgccaac gcctgttaaa tctggtgcaa taccgccgct gacaatcaac
    20221 gccacgccat gacgggcgcg ttccgcgtaa aacgctgcca gccgttcagc gccgtccggg
    20281 tattcctcca gtcccgtgtg cattgaaccc atcagtactc ggttttttaa cgtggtgaag
    20341 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
    20401 aattgttatg tggtcggatg agttctaatt tagccgtcgc cagagaaaag ggaaaaaggg
    20461 agtgcggggt ttgtgatggg attcaaaaag aatacgccca ttggaaatgc cggatacgac
    20521 gcttgtcgcg tcttatctgt atatggacac ctctcgttgt gcaagtacgg tgttcgaaaa
    20581 attaataggt aacgtggtgg tcgtcctgac aggacggcag gctacggaga tagtcaatca
    20641 cgctatcttt agcccgctca tccgggggca tcttcgacca tttcagaatg acttcgtcga
    20701 taatgggggc tttttggtcc ctttctgtct tgccaggacg gagggcaatt gcgggccagg
    20761 cgcatgttcc gcactctgat ttccatccat cagtaagggc tgtgaagacc tgaaacttcg
    20821 gatgtttttg acatgggggc tggtcctttt ggccactttc cgcctaacc
//
LOCUS       DARNVM010000079        20689 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.079, whole genome shotgun sequence.
ACCESSION   DARNVM010000079 DARNVM010000000
VERSION     DARNVM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.079"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(106..1755)
                     /gene="actP"
                     /locus_tag="SI621_002629"
     CDS             complement(106..1755)
                     /gene="actP"
                     /locus_tag="SI621_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HEI4065333.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLLTAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSVTDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1752..2066)
                     /locus_tag="SI621_002630"
     CDS             complement(1752..2066)
                     /locus_tag="SI621_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEI4065334.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2293..4251)
                     /gene="acs"
                     /locus_tag="SI621_002631"
     CDS             complement(2293..4251)
                     /gene="acs"
                     /locus_tag="SI621_002631"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEI4065335.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLIEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVISDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4644..6080
                     /gene="nrfA"
                     /locus_tag="SI621_002632"
     CDS             4644..6080
                     /gene="nrfA"
                     /locus_tag="SI621_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HEI4065336.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQKAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6125..6691
                     /gene="nrfB"
                     /locus_tag="SI621_002633"
     CDS             6125..6691
                     /gene="nrfB"
                     /locus_tag="SI621_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HEI4065337.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6688..7359
                     /gene="nrfC"
                     /locus_tag="SI621_002634"
     CDS             6688..7359
                     /gene="nrfC"
                     /locus_tag="SI621_002634"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HEI4065338.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7356..8312
                     /gene="nrfD"
                     /locus_tag="SI621_002635"
     CDS             7356..8312
                     /gene="nrfD"
                     /locus_tag="SI621_002635"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HEI4065339.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLLLWLAKIFEHDLLALQQRWLPKLGFVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8332..10050
                     /locus_tag="SI621_002636"
     CDS             8332..10050
                     /locus_tag="SI621_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEI4065340.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLFALISLASLALYGW
                     RARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10043..10426
                     /gene="nrfF"
                     /locus_tag="SI621_002637"
     CDS             10043..10426
                     /gene="nrfF"
                     /locus_tag="SI621_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HEI4065341.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQERALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10423..11019
                     /gene="nrfG"
                     /locus_tag="SI621_002638"
     CDS             10423..11019
                     /gene="nrfG"
                     /locus_tag="SI621_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HEI4065342.1"
                     /translation="MKPPQIPVKMLTTLTILMVFLCVGSYLLSPKWLAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRTNPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11361..12674
                     /gene="gltP"
                     /locus_tag="SI621_002639"
     CDS             11361..12674
                     /gene="gltP"
                     /locus_tag="SI621_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HEI4065343.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12753..13442)
                     /locus_tag="SI621_002640"
     CDS             complement(12753..13442)
                     /locus_tag="SI621_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEI4065344.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13536..15683)
                     /gene="fdhF"
                     /locus_tag="SI621_002641"
     CDS             complement(13536..15683)
                     /gene="fdhF"
                     /locus_tag="SI621_002641"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15264..15266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEI4065345.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15881..17347)
                     /gene="mdtP"
                     /locus_tag="SI621_002642"
     CDS             complement(15881..17347)
                     /gene="mdtP"
                     /locus_tag="SI621_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HEI4065346.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMHWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(17344..19395)
                     /gene="mdtO"
                     /locus_tag="SI621_002643"
     CDS             complement(17344..19395)
                     /gene="mdtO"
                     /locus_tag="SI621_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HEI4065347.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19395..20426)
                     /gene="mdtN"
                     /locus_tag="SI621_002644"
     CDS             complement(19395..20426)
                     /gene="mdtN"
                     /locus_tag="SI621_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HEI4065348.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAGLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGT
                     SAVANLEPQ"
     gene            complement(20445..>20689)
                     /locus_tag="SI621_002645"
     CDS             complement(20445..>20689)
                     /locus_tag="SI621_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065349.1"
                     /translation="LKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWFFCAIASLILTL
                     ITRRIIQRANINLAFVGIIYTALFALYAMLLWLAFF"
BASE COUNT         4638 a         5513 c         5659 g         4879 t
ORIGIN      
        1 tcgctcattt tttaatgcgt ttatttgttt aatattatgg gaaaaggtga tgcattttgc
       61 agaggaagag tattccccgg tcaggcgacc ggggaagggg agagattaat gcgcgcggcc
      121 ttgctcaacg ccaaagccgg tctgggagcg gataaactgc gcacggaaca gttcacgctc
      181 acgcgcgcct tccgctgagt tatcggttac cgagaagaac cagatgccga ggaatgccac
      241 gctgatagag aacagcgccg ggtattcata cgggaagatg gctttttcgt gaccgaggat
      301 ctgtacccaa atcgtcgggc cgaggatcat cagtaccact gcggtaagca gccccagcca
      361 gccgcccatc attgcgccac gcgtggtcag ttttgaccag tacatggaaa gcagaatgat
      421 tgggaagtta cagctcgctg cgatggcaaa cgccagcccc accataaagg cgatgttctg
      481 attctcaaac agcacgccga ggataatggc aatcacgccc aaaatcagga cggtgatttt
      541 cgatacccgc agctcttcac gttcagtcgc gccttttttg aagacgttag cgtacaggtc
      601 gtgagacacc gccgatgcgc ccgccagcgt cagacccgca accaccgcaa gaatggtggc
      661 gaaagcaacc gctgaaataa agccgaggaa caggttgcca cccactgcat tcgccaggtg
      721 aactgccgcc atgttgttac caccaatcag atggcccgcc gcgtctttat attccggatt
      781 cgcaccaacc agcatgatcg cgccgaagcc gataataaag gtcagaatat agaagtagcc
      841 cataaatccg gtagcgtaga acacgctctt acgcgcttcg cgggcatcgc tgaccgtaaa
      901 gaagcgcatc agaatgtgcg gcaagccagc cgtaccaaac atcagtccga gccccagcga
      961 cagtgctgag atcggatctt tcaccagccc gcctggcttc atgatgtcga cacccttcgg
     1021 gtgtaccgcc atcgcttcgc tgaacagatt gttgaagcta aagccgacgt gtttcatcac
     1081 cataaaggcc ataaagctgg caccgaacag caacagcacg gctttgataa tctgtaccca
     1141 ggtggtcgcc agcatgccgc cgaacaggac gtacatcatc atcagcacgc cgaccagcac
     1201 taccgcaatg tgatagttaa ggccaaacag cagctcgatc agtttacctg cgcctaccat
     1261 ctgagcgata aggtaaagcg ccaccaccac cagagaacca caggccgaaa gaatacgaat
     1321 cggcccttgt ttcaggcggt aagaggctac atcggcaaag gtgtagcgtc ccaggttacg
     1381 cagacgttcg gcaatcagga acagaatgat tggccagccc accaggaagc ccaacgagta
     1441 gatcagcccg tcatagccgg aggtaaacac cagcgcggag atccccaaga atgaggcggc
     1501 ggacatatag tccccggcaa tcgccagccc gttctgaaaa ccggtgatat tgccgcctgc
     1561 cgtgtagtag tcattacgcg aacgtacgcg ttttgacgcc cagtaggtaa taccgagcgt
     1621 aaacacgacg aaaatcagga acataataat cgcctgccag tttgttggct ggcgctctac
     1681 ggccccgcta atagcatccg cggcgttagc tgcgaaaggg agtgtggcgg caagcgccgt
     1741 cagaactctt ttcatgatgc ttgtacctca tgcaggactt cattattaag acggtcgaat
     1801 tcgccgttcg cccgccagat gtagatacca gtgagaacaa aggagatcac aattacccca
     1861 acaccaatcg gaataccgcg tgtgacgctg gtgttcggat tcagcggagt gcccagccag
     1921 ccgggcgcga aggcgatcag taaaataaag ccgatataaa ctgccagcat aataatcgac
     1981 aggatggtgg caaaccgttg ccgtttttcg actaactccc tgaaatgcgc attgtcttct
     2041 atccgctgat aaatcgtacc attcatcaca gattctccag aggttccccg tcgtctttca
     2101 cgccgcaggt tcgtcagccg cactcgcctc ccccagtcac atagttttct atgctcctgg
     2161 ggagatgctc gcttgcttcc tgcctgcaac gcgaaatact taggggaatt tttatatttg
     2221 ccctttccca ctataaagaa aggggcgtgt agaccggata aggcgttaaa cgccgcatcc
     2281 ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca gcttctcgac
     2341 cacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat
     2401 tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg aatcggtcca
     2461 gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt tgcggacttc
     2521 tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacgtagg cgtagatcgc
     2581 ctgaccttta atattgtgcg gaataccgac gacagcggct tcggcaatct tcggatgtgc
     2641 caccagcgcc gactcaatct ctgccgttcc cagacggtga ccggagacgt tcagcacgtc
     2701 gtccacgcgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc cgtcgccgct
     2761 gaaatacata tttttgaagg tggagaagta ggtctgctca aaacgttcgt gatcgccaaa
     2821 cagcgtacgc gcctgacccg gccaggagtc ggagatcacc agactgcctt cggtggcccc
     2881 ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga acggacgtgt
     2941 tgccgaaccg gctttcagct cggtagcgcc aggcagcggg gtgatcatga aaccgccggt
     3001 ttcggtctgc caccaggtat cgaccaccgg gcatttctcg ttgccgattt ttttccagta
     3061 ccactcccac gcttccgggt tgattggctc gcctacggaa ccgagaatgc gcagcgacga
     3121 acggtcagtg ccttcgatcg ctttatcgcc ttccgccatc agcgcgcgga tcgccgtggg
     3181 cgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac gggcaggcgt
     3241 cggccagttc ggtacgcctt caaacatcag cgtggtcgcg ccgcaggcca acgggccgta
     3301 cagcaaataa ctgtgtccgg taacccaacc cacatcagcg gtgcaccagt agatatcgcc
     3361 cggatgataa tcaaagacat atttaaaggt cagcgccgca tacaccagat aaccgccggt
     3421 agtgtgcagc acgcctttcg gcttcccggt agaaccggag gtgtagagaa taaacagcgg
     3481 atcttcggcg ttcatctctt ccgcctggtg ctgatcgctg gcttgctcaa tcaggtcgtg
     3541 ccaccacaga tcgcgccctt cctgccagtc aattttcccg ccagtacgct tcagtaccac
     3601 cacatgttca atgctggtga cgttcgggtt tttcagcgcg tcatcaacgt ttttcttcag
     3661 cggaatacta cgcccggcgc gcacgccttc gtcggaagtg atcaccaacc gtgagttaga
     3721 atcgataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatta ccgaatgcac
     3781 cgcgccaatg cgggcgcagg ccagcatcgc gaccgcggct tccggcacca tcggcatata
     3841 aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg cgaagcggca
     3901 gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt cgtcgccttc
     3961 ccagatgatg gcggtacgat cgccgttttc ttgcagatgg cggtcaaggc agtttgccgc
     4021 cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg caaaggaggt
     4081 gtttttcacc ttctggtaag gtttgatcca gtcgagaatt tttccctgtt cgccccagaa
     4141 ggtatctggt gcgttaatag attgttgata catcgcctcg tactgctgag ggtttatcag
     4201 gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttgactca tgcttttgtt
     4261 ctccttgtag gatgttaata atatgtggca taagagttaa atgtaggggt attggcagtt
     4321 ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata aacggcaggg
     4381 taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg cagttatgca
     4441 tgctgttgaa aaagaggaag atactgacta actctaaagt ggtattttac atgcacttac
     4501 aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg cgagcaatgt
     4561 catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca ttgcaacaat
     4621 ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc cgtatcttca
     4681 gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg gcagctcccg
     4741 caaaacctgt aactgttgaa gcgaagaatg aaacctttgc cccgcaacat cccgatcaat
     4801 atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg gcggaagatc
     4861 cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac aagccgcgtg
     4921 gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg caccggtgcg ccgaaaaacg
     4981 ctgaagatgg tccgctaccg atggcatgct ggagttgtaa aagcccggat gtggcgcgtc
     5041 tgatccagaa agacggcgaa gatggctact tccacggaaa atgggcgcgc ggcggcccgg
     5101 aaatcgtcaa caacttaggt tgtgccgatt gccataacac cgcctcacca gagttcgcca
     5161 aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg
     5221 gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc
     5281 atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg gatgacggca
     5341 tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac tggactaact
     5401 ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc tggacagcgg
     5461 gcattcatgg caaaaacaac gtgacctgta tcgattgcca tatgccaaaa gtgcagaacg
     5521 ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac ttcgcccaga
     5581 cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc gcggaacgta
     5641 agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac gctcacttcg
     5701 aagcgaaagc ggcgctggat gcaggcgcga cggaagccga aatgaagccg attcaggacg
     5761 atatccgtca tgcccagtgg cgttgggatc tggcgatcgc ttcccacggc attcatatgc
     5821 acgcaccgga agaaggtctg aggatgctcg gtacggcgat ggataaagcg gcggatgcgc
     5881 gcactaaact ggcgcgactg ctggcaacca aaggcatcac ccatgaaatc cagatcccgg
     5941 atatctcgac caaagagaaa gcccagaagg ccattggcct gaacatggaa caaatcaagg
     6001 ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagagcag gcacgtaaaa
     6061 acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac acaatggagt
     6121 gaatatgagc gtattacgtt cgttattaac tgccggggtg ctggcgtcgg gcctgttgtg
     6181 gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctggtgatc gctacgaagt
     6241 tacccagcag cgtaacccgg atgccgcctg tctggactgt cataagccag ataccgaagg
     6301 tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaactgccgg tcacctgcac
     6361 caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt
     6421 taacgagccg atgtacaagg ttggagagca gaacagcgtc tgtatgtcct gccatctgcc
     6481 ggaacagttg caaaaagcgt tctggccaca cgatgtccac gtaaccaaag tggcgtgcgc
     6541 cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg acaaaggacg
     6601 gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact ttaacccggc
     6661 gtctgttccg ttgcttaagg agcagccatg acctggtcac gtcgccagtt tctcaccggc
     6721 gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa aacgttgaat
     6781 atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgtatcgg ctgtaccgcc
     6841 tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt
     6901 attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct
     6961 tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt
     7021 gatgccgcta gcgggattgt cgatgttaac ccggatctct gcgtcggttg tcagtactgc
     7081 atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac agcggacaaa
     7141 tgcgatttct gccgtaagac caatttgcag gccggtaagc tgcccgcgtg cgttgaggct
     7201 tgcccgacga aggcgctgac gtttggcaat ctggacgatc ccaacagtga gatttcgcaa
     7261 ctgctgcgcc agaagcccac ttaccgctac aagctggcgc tgggaaccaa accgaagctg
     7321 taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc gcatttcatt
     7381 ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt ggtatttctg
     7441 ccggtctggt gacactggcc gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag
     7501 acagtacgtt gttgcgcacc acgctgattg tcgggcctgg cgcggtgatc cttggtctgt
     7561 tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg ttccactaca
     7621 gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg gtggtgctgt
     7681 tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa cgctggttgc
     7741 cgaagctggg gttcgtgcaa aaggttctga gcctgctgac gcccgttcat cgcggactgg
     7801 aaacattgat gctggtgctg gcggtgctgc tgggggctta taccggcttt ctgctatcgg
     7861 cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc ctcttctccg
     7921 gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa cgcagtaacc
     7981 cgcattccac ggaagcgcac tttgtacacc gtatggagat ccctgtggta tggggcgaaa
     8041 tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt aaagtgcgtg
     8101 cgctggtggc ggcattgggc ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg
     8161 ggctggggtt gattgtgcca atgttgctca aaccgtgggt caatcgcagt tccggcattc
     8221 ctgccgtgct ggcggcgtgt ggggccagcc tggtcggcgt gttgatgttg cgatttttca
     8281 ttctctacgc cgggcagtta acggtggcgt aagccagaac agaggtggtt tctggacgta
     8341 ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc
     8401 ccgttgacgg ccttcgcggg agtgcggttg cgctggcctg ccatgatgcg actcacttgc
     8461 atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact gacgtactgt
     8521 tttctcatca gcgatttctc ggtcatttat gtcgcccaac atagctacag cctgctgtcg
     8581 tgggaactca aactggcggc ggtgtggggc ggtcatgaag gttcgctgct gctttgggtg
     8641 ctgttgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca aaccgatccg
     8701 ctatttccgc tgacgctagc cgttttatct ctcatgctcg ccgcactgct actgtttgtg
     8761 gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg ccgcgatctc
     8821 aatccgatgc tgcaacatcc cggtcttatc tttcatccgc cgctgcttta tctcggctat
     8881 ggcggtttga tggtggcagc gagcgtggcg ctggggagtt tactgcgcgg cgagtttgat
     8941 ggtgccagcg cccgaatttg ctggcgctgg gcactacctg gctggagcgc attaacggcg
     9001 gggatcatcc tcggttcctg gtgggcctat tgcgaactgg gctggggcgg ctggtggttc
     9061 tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc gctgctgcac
     9121 agtttgtccc tgacacgcca gcgggggatt ttccgccact ggtcgctgtt actggcgata
     9181 gtcactctga tgctgtcgct gctgggtacc ttaattgtcc gttctggcat tctggtttcg
     9241 gttcatgcgt tcgcgctgga taacgtccgc gccgtgccgt tgttcaccct gtttgcactg
     9301 attagccttg cgtctctggc tctgtatggc tggcgagcgc gggacggtgg cccggcggtg
     9361 catttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct gttttgcgca
     9421 gtgctattga tcgtgctggt gggaacgctt tatccgatga tttacggcct gctgggctgg
     9481 ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt tggtctgttg
     9541 atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca gcttccggcg
     9601 ctggtagctc atgcgggcgt gctgttattt gccgcgggga tcgtggtctc cagcgtcagc
     9661 cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc aggatacacc
     9721 ttccgttttg agcgcctcga tctgcaagcc agaggcaatt acaccagcga aaaagcgata
     9781 gtggcactgt ttgaccatca gcaacgtatt ggtgaactga cgccggagcg gcgtttttac
     9841 gaagcacgcc gtcagcaaat gatggagccg tcaattcgct ggaacggcat ccatgactgg
     9901 tatgcggtta tgggggagaa aactgggccg gatcgttacg cttttcgttt gtatgtacaa
     9961 agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc attgttaagc
    10021 ggatggcggg ggaggaagcg cgatgaataa aggacttctc acgctactgc tgttatttac
    10081 ctgttttgcg cgcgctcagg ttgtagacac ctggcaattc gccaatccgc aacaacagga
    10141 acgggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc agaacttact
    10201 ggaatccaat gcgccggtgg cagttagtat gcgccatcag gtttacagca tggtggcgga
    10261 ggggaaaagt gaagtcgaaa tcattggctg gatgaccgaa cgctacggag attttgttcg
    10321 ctataaccca ccgttaacgg gtcagacgct ggtattgtgg gctctgccag tggtgttgtt
    10381 actgctgatg gcactgatcc tctggcgagt gagggcgaag cgatgaaacc gcctcaaata
    10441 ccggtgaaaa tgctgacaac actcacgata ttgatggtat ttctctgtgt cggcagttat
    10501 ctgctaagtc caaaatggct ggcggtgcgt gcggagtatc agcgtcagcg cgatccgcta
    10561 catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca ggataaaatc
    10621 cgcactaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtatta tctgtggcaa
    10681 aacgattaca gcaattcgct gctggcttac cgtcaggcgt tgcaactacg tggtgagaac
    10741 gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggctagcca gcatatgacc
    10801 gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga aatcaccgct
    10861 ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc catcgaacta
    10921 tggcaaaaag tgatggatct caactcaccc cggatcaaca ggacacagct ggtcgagtcg
    10981 attaatatgg cgaaattgtt gcagcggaga tcagattaat catctggtga tggagattat
    11041 gttattgagc tgcataaaaa ataatcgaat gaacatatgc caaaaataat caccaatcag
    11101 tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt cattaatcct
    11161 gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc
    11221 gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca aaccaacgca
    11281 acacaattca tgccctggca gtatgtcacg ttctcacgtt tctgaacggg gaacggcgct
    11341 ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg cagattctgt
    11401 ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc gacagccgcg
    11461 actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat ctgatcaaaa
    11521 tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt gttggtgatg
    11581 ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg atcaccaccg
    11641 tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc ggggtggata
    11701 tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa gcggtacaaa
    11761 gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac attgtggcgt
    11821 cgatggcgaa aggcgaaatg ctgccgatca tcttcttctc ggtgctgttt ggtctggggc
    11881 tttcctccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc tccatctctg
    11941 aaaccatgtt taaagtgact cacatggtga tgcgttatgc gccggtgggt gtgtttgcgc
    12001 tgattgcggt gacggtggct aactttggtt tctcgtctct gtggccactg gcgaaactgg
    12061 tgctgctggt gcatttcgct attctgttct tcgcgctggt cgtgctggga attgtggcgc
    12121 gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag ctgattctgg
    12181 cgtactccac tgccagttct gaaagcgtgc tgccgcgaat tattgagaag atggaagcct
    12241 acggtgcacc ggcgtcgatc accagtttcg tggtgccgac cggttactct tttaaccttg
    12301 atggttcgac gctgtatcaa agtattgccg ctatcttcat tgcgcagttg tatggcattg
    12361 acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc tcgaaaggga
    12421 ttgctggcgt gccgggcgtg tcgtttgtgg tgctgctggc aacgctgggt agcgtaggta
    12481 ttccgctgga aggtctggca tttattgccg gtgttgaccg tatcctcgat atggcgcgta
    12541 ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag tgggaacaca
    12601 aatttgaccg taagaaagcg ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa
    12661 ctgcggatca ataattgaag attgccgggg atatccaccc ggcaatgtgt ggatgcctga
    12721 tgcgacgctt aacgcgtctt atcaggccta ccttaaccgt tagttaattt ttcaaactct
    12781 tcacacccgg tatcaaaccc ttccatacag ctcagattta accagtgcag cgccttttgc
    12841 ttgttcttct cgataaaacc ttcttcacca tttaagaaca tcattcccgc ccagtactcg
    12901 gaataaccgg ttcgggaaat cgcagagctg cgtttgaaat accaggttgc cttgtcatcg
    12961 tcggccttaa tgcccacgcc gttggcgtaa atcaaaccaa gcagcatttg ggcatcgacg
    13021 gcagagtcgt tctccagatc ttccgaagcg ttttctaaca gcgaaatcgc ctttggataa
    13081 tccggtttac ccgcttgcgt atttaccaga atatgcgcca gcgttacttc accttcttta
    13141 ctccccgctt gcgccgcttt ctctgcaaga acttttgctt gtggatagtc cagactgact
    13201 ggattggtga ttttgatctg cgccaacagc gcgcaggcat cagcatcacc gctgtcggcg
    13261 gctttctgtg cccaatattc ggctttgctc aaatcgccgg agctaaacca gctgtcggca
    13321 agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct ttaaatactg gctgccaggc
    13381 tcggagtcgt tcgcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat aatttttttc
    13441 attttgataa tacgattggg tagggagtaa ccagtataaa gagttcgaat ggataaaaaa
    13501 acagcctccg aaaggaggct gtagaaagga ctgtattacg ccagtgccgc ttcgcgcagg
    13561 cgagttttca acttgttgta ctcgtcaatc acgtactgct cggcggcgcg ctgatcggcg
    13621 atcggctcga cgcgcacggc gcagtatttg tattccggcg ttttcgtaat cgggcttaag
    13681 ttttcggtaa ccagctcgtt acaggcacca atccaccact ggtaggtcat gtaaatcgcc
    13741 cctttgttcg gacgatcgct gacctgcgca cgggtgataa ttttgccttt acgcgagtgc
    13801 acccaaacca atgcctcatc ttcaataccc agacgtttgg cgtcttcggt attgatttgt
    13861 gcgtagccag gttcatcagc cagcgccgcc agtgccgcac agttaccggt catcgaacgg
    13921 caagagtagt gaccaacttc acgcaccgtt gacagtacca tcggatattc gtcggtgagt
    13981 ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg ccagaccgtt cggggtatca
    14041 aacttctctt taaacaaata agaagtcccc tgatcggcat ctgaagtatc gcgacaaggc
    14101 cactgaatga agcccagttc gcccattttc tcgtaagtcg caccgtagaa atccgggcac
    14161 agatgacgca actcatccca gatctcctgg gtgttgttgt agtgcatcgg ataacccata
    14221 cgagtggcaa tttcactgat gatttgccag tccgttttca gatcccactt cggctcaacc
    14281 gccttgaaga aacgctggaa gccacggtca gccgcagtaa acacgccttc atgctcgccc
    14341 cacgacgttg acggtaaaat gacatccgcc gccgacgcgg ttttggtcat aaagatgtcc
    14401 tgaacgatga ccagttccag atcttcaaag gctttacgta ctgccgacag ctccgcgtca
    14461 gtttgcagcg gatcttcgcc cataatgtac gcggcacgca cttcgccatg cgctgcgcgg
    14521 tgcggcagct cgctgatgcg atagccggta tgagcaggca ggctttccac gccccaggct
    14581 ttggcgaatt tctcgcggtt agccggatct ttcacgtact ggtatcccgg atacgtatcc
    14641 ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct gaccacgaac cgggtttaca
    14701 cccgcatgcg gcttaccgag gttaccggtc agcatcgcga ggctggtcag agaacgcacg
    14761 gtttccacgc cctggtagaa ctgggttaca cccatgcccc acaggatggc ggcgcttttc
    14821 gcctgggcat acatccgtgc cgcctgacga atctcactgg cgctgacgcc ggtgatatct
    14881 tcaaccgact ccggcgtgta gccttcaacg attttacgat actcttcaaa gccttctgta
    14941 cgggaagcga cgaatgcttt gtcgtacaga ttttcttcaa taatgacatg gcccatcgca
    15001 ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt gcatgtccgc aatgcgcgcg
    15061 gtttcaattt tgcgcggatc gcagacgata attttcgccc cgttacgttt agcgttaatt
    15121 acgtgattcg ccacgattgg gtgggaatcc gccgggttgt acccgaaaac gaacactaaa
    15181 tcggtattat caatttcgtt aatagcattg ctcattgcgc cattaccgac cgattggtgc
    15241 agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt attggtacca
    15301 ataacggcgc gcgcaaattt ttgcattaca tagttggttt cgttacccgt accacgcgag
    15361 gagccggtcg tctggatggc atccggaccg tacttctctt tgatggcgct caggcgctcg
    15421 gcaacgtaat tcagtgcctc atcccaggaa acaggttcga gtttgccgcc acgctggcga
    15481 cggatcatgg gggttttcag gcgcggggtc aggatctggg tatcgttaat gaaatcccag
    15541 ccataataac ccttcagaca cagggtaccc tggttggttt tcccctgcgc tgcctccgcc
    15601 cggacgattt tgccgttatc gacgaccagg ttgattttgc aacctgatgc gcaatagggg
    15661 caaaccgtga cgactttttt catcggtctc gctccagtta atcaaatcac gcatacgcgc
    15721 tctcgactac agtatgcatc ttttatgcca cattttattt ggggtcattc cctgatatta
    15781 cgggcactat ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt tgacgtgacg
    15841 aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt ttattttttc tcgacgacgg
    15901 gacttgcctg atacccgccg cccagcgatt tcatcaactg aatgctttgg atcacccggc
    15961 ggctgtccag catcagtaat gacatctcct cggcaagcac tggcaaccgg gcttcggtgg
    16021 cctgtaagcg gctggttaag ccgcgttgat aggcggcctc ggcagcgcgc tgggtgaagc
    16081 gcgttgcttc cacgcgttca gcctgcatct ctcgctcgtc gttgagcgtt tgcagacgcg
    16141 tgccgttgac ggcaacgtcg cgcaccgcat tcagcactga ctggttgtaa cgttcaatca
    16201 tcatgttgct ggcggcgcga gtgccttcga gattggcatt caaccgtcca ccgtcaaaca
    16261 gcggcaattt cagacctggg ataaagttga actggcgact ggtttttttg aataaggtat
    16321 ccaggtggat agaatccagg ccgaaaaacg ctttgatatc aaagctcgga tagaacagcg
    16381 cccgcgcgga atccacctga tctaatgacg cctgaacata ccagtgcata gcttgcagat
    16441 ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg aataccggtc tggacttgcg
    16501 gtaatgccac cggtttgatc tccggcatat cgctggctcc cgcgccaatc aatgcacgca
    16561 gagattctcg cgtttcggtg atttgccctt taacggcagc aatttgttta tcgaccgcca
    16621 gaatctgtgc ccgcgcgccg tggaaaggca cttgcgcttc cagaccgtgc gccactttac
    16681 tctggtgcgc tttcaccgcg taatcaatca catcgcgtgt ttgttctaac agatcgagca
    16741 tctgatagct ggcctgcata ctgtaataaa gctgcgctac gcccgtggtc agcgatagct
    16801 ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg gcaaccgctg
    16861 agcgatgcac accccacaaa tcaagatcca gtccggcaaa caaaccgact gtggcttccg
    16921 tatagtacgg cccgtccata cccagtgctg gcgcatccat cgcataaggg cttaaaaagc
    16981 cgttcgccga gacacgttgg cggttgagca tccctaacgc tgccacctgc aactgtgaac
    17041 cagcatctaa caaatcggcc tgggactgcg ctttttcttc ccgcagtttc gcttcggcga
    17101 gggtgtgtga accacttagc gtccgttgga tcagggcatc caactgcggg tcattgagtt
    17161 gtttccacca ctgcgcctgc ggccagccgg agctggcaag atgaatatca tcggccagtt
    17221 tgatttgttc cggtttgagc tgttgatgag gtgcagaatc cttacgtacc agggcacagc
    17281 cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa agttgacgat
    17341 tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc gttaatgccg gggctgtcca
    17401 gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc tgttttaata aggtgggcag
    17461 gatggcctgc gaggcgggtg tttcttcaag cgttatttga ggagaacggc tgagtgcggt
    17521 tgccagaccg gcggcgtatt tctccagtgc gtcggcaaac tgacctgctc tgtccggctg
    17581 tagtgcgtta tccagcgcct gcgccgagtt ccaggttgca tcccaggcgt gaagaatatc
    17641 gcggccctga cgaataaccg tttgcgaacg ttcaatcagt aaagcgcgtt cttcgctgtc
    17701 cagttgacgc tccaacgcca cgcgttggca catctcttca caggcattaa acgccgcatg
    17761 cagacctata cgaatttgca aataagtgcg cagagctgtg acttcctgct ggcgtgggat
    17821 ccgcattact ttactcaaca tacccagcgc gccagccagt ttttgcggca gtgtgcgcgc
    17881 ttcactttca ggccagacaa aggtgtaaat caccgcggac accacggtac caatgatgat
    17941 acccagggcg cgatcgcgaa tttccaccag gtcgtacacc gggccaaaaa cgttttcgag
    18001 cgtggcgagc gcgaaggtga ccaccatctg tgtgccgata taagaagagc gttcagagct
    18061 ggtggcaatc catgcgccca acagaaaaat cggtgccagc acaaatagca attcgacaat
    18121 attgtccagc cagggcatga ccagtaacgt gaataacagc gccagaattg caccgcagaa
    18181 ggcaccgcca aaacgcagcg ccattttctg atacgacgaa ccaacattcg ggttagcgac
    18241 gatcacgcag gtcagcatac aggtgtgaat gccttcccag tccacgccgc tgtagaaggt
    18301 gtaacagatc aaacaggcga gcagcgtttt taccgcgtag cgcatatagt ctggattggt
    18361 aaaagcgtcg gcgaccattg atggcggttt ggtggcgggc gttggcggcg tattcgggtc
    18421 catctgaccc agttgcaata acgtctggca gatattctcc aggttacatt cccgtgccgc
    18481 catcgcttca ctttcagtga tccgccagtc gctttgccag cactgacctt cggcaatggc
    18541 atgctgcagt ttgttgattt ctgaggctaa tttttgccga aattcaataa ttgcctgaga
    18601 atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg ttaccgttgc
    18661 cacgcagctt tgccaccatg cgctctgagt tcgccagttg gcatcgtccg cgagacaaaa
    18721 tacattcagt ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg gtagcggtgt
    18781 gaggctgttc gtcaggtggc taatggcatc atcaagccga tcattaagcg cctgatgcat
    18841 ctgcgtgata gcgcggctgg gaaaccacag cacgccgatt aacgtcatca ataacgtcgg
    18901 gtagaggccg acaacgatac accacagggt taagcgcacg accacttccg gatagtcgag
    18961 catggcgggg aaggtttgcc cgtaaatggc gacaatggcg acagcgaaaa agaccagtcc
    19021 cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg cgatgatcaa
    19081 tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag
    19141 caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat
    19201 accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat
    19261 cagaatcacc agtaggcagc ctacccagag ttgcacggtc tgcggcacac gacctggccg
    19321 ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga
    19381 gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagtgcc gatgcggaac
    19441 atttccgggt caggtttatc aaccatgatt ttgaccggaa aacgctgggc aacgcggacc
    19501 cagttaatag aacgagacac tttcggcagg ccgcccagca ccaggccgcc gtcatccggt
    19561 agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct gtcgctcatc
    19621 aggcgaatgg ttgcgggtgt acctgagcga atatttttca gatcagtttc gcggaagttg
    19681 gcgatcacgt accagtgacg agtgtcgatt agggtgaaaa tagggcgcat ggcagaagca
    19741 aattgcccga cggaggtttt gagggaaatg acccggccat caaacggcgc gcgaacggtc
    19801 gccatttcca gatgcagttt cgtcaacgca atatccgctt ccactgccgc acgttgcgcc
    19861 accagcgcat ccacgccgct gacggcgctg gcggctgact gcgcctgcaa caatccggca
    19921 ttaagatcgg cttctgcggc gcgctgcgcg gttctcgcgc ggtcaacgtc ttccgctgag
    19981 acgaaacctt ctttcagtaa tggctcggtg cggcgtaatg tatcactggc ctgtttcgcg
    20041 gcggcacggg ctttttctac cgtggcatta accgagtcgg caccaaactg ttgcgcgtca
    20101 acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcggctttc
    20161 gccagattgg cctcgtatgg gcgcgggtcg atgcgaaaca gcaaatcgcc ctgtttcacc
    20221 gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg caccacatca
    20281 atggtatctg ctgacgcgta ggcgtcatta gttgatggcg cactgtctac gcgccagata
    20341 acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa tttactgcga
    20401 ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc
    20461 cagagcagca tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga
    20521 ttgatatttg cccgctgaat aattcgcctc gtgatgagcg tcaaaataag gctggcaatg
    20581 gcgcagaaaa accagctggg ataataagcg ccgatcaccg ggattgccgg agataatgag
    20641 catcccgaaa ccatgaagac gggaattatc caggctgttt ttttgagtt
//
LOCUS       DARNVM010000080        20410 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.080, whole genome shotgun sequence.
ACCESSION   DARNVM010000080 DARNVM010000000
VERSION     DARNVM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.080"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..526)
                     /locus_tag="SI621_002646"
     CDS             complement(<1..526)
                     /locus_tag="SI621_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HEI4065350.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEYGQYSVILLVEGFLPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPE
                     ALRRFEAMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAASSQ
                     TASANSATAAKKSET"
     gene            complement(591..1190)
                     /locus_tag="SI621_002647"
     CDS             complement(591..1190)
                     /locus_tag="SI621_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HEI4065351.1"
                     /translation="MRKLCAVILSTVVWLVVAGTPASAAEHQSTLSAGYLQTHNDMPG
                     NDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDIKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1258..4737)
                     /locus_tag="SI621_002648"
     CDS             complement(1258..4737)
                     /locus_tag="SI621_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEI4065352.1"
                     /translation="MGKGSSKGHTPREAKDNLKSSQMLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLVSVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGRYTLTVRAVNTRGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAIYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWAQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VRKNGAEIYNRTALDNTLIYSGVIDMSAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            complement(4798..5430)
                     /locus_tag="SI621_002649"
     CDS             complement(4798..5430)
                     /locus_tag="SI621_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEI4065353.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSN
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVISQEISTADEGDGGQVVVIGR"
     gene            complement(5367..6110)
                     /locus_tag="SI621_002650"
     CDS             complement(5367..6110)
                     /locus_tag="SI621_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065354.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSKADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHAQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGEMLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            complement(6115..6813)
                     /locus_tag="SI621_002651"
     CDS             complement(6115..6813)
                     /locus_tag="SI621_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEI4065355.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNRDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     SVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCTFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(6813..7142)
                     /locus_tag="SI621_002652"
     CDS             complement(6813..7142)
                     /locus_tag="SI621_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065356.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(7139..9700)
                     /locus_tag="SI621_002653"
     CDS             complement(7139..9700)
                     /locus_tag="SI621_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEI4065357.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYESA
                     LKKPKQSGVRGSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GKFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            complement(9693..10127)
                     /locus_tag="SI621_002654"
     CDS             complement(9693..10127)
                     /locus_tag="SI621_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEI4065358.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDMADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(10109..10531)
                     /locus_tag="SI621_002655"
     CDS             complement(10109..10531)
                     /locus_tag="SI621_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEI4065359.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLAWLKEQEKKAESSGN
                     LQVSVEDLIRGGAFLVAMSLWHNHPQKTKLPSMNEAITQIEQEVLTTWPTEAIAQAEN
                     VVLRLSGMSEFVVNNAPEQADDAGPAEPVSAGKCSTVS"
     gene            complement(10547..11287)
                     /locus_tag="SI621_002656"
     CDS             complement(10547..11287)
                     /locus_tag="SI621_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065360.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEINVTAS"
     gene            complement(11295..11690)
                     /locus_tag="SI621_002657"
     CDS             complement(11295..11690)
                     /locus_tag="SI621_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065361.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     RAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(11687..12265)
                     /locus_tag="SI621_002658"
     CDS             complement(11687..12265)
                     /locus_tag="SI621_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065362.1"
                     /translation="MAIQGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERVRLKRATVKNPQARIKVNRGDLPVIRLGNARVILSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRRHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(12277..12630)
                     /locus_tag="SI621_002659"
     CDS             complement(12277..12630)
                     /locus_tag="SI621_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065363.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVHGVFD
                     DPESLGFASSGIRIEGSNPSLFVLTDTVCAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAASRRR"
     gene            complement(12623..12997)
                     /locus_tag="SI621_002660"
     CDS             complement(12623..12997)
                     /locus_tag="SI621_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065364.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERETAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(13049..14077)
                     /locus_tag="SI621_002661"
     CDS             complement(13049..14077)
                     /locus_tag="SI621_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEI4065365.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPSQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPDGMLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(14135..14482)
                     /locus_tag="SI621_002662"
     CDS             complement(14135..14482)
                     /locus_tag="SI621_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEI4065366.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSPTPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGRALSHAALP"
     gene            complement(14519..16024)
                     /locus_tag="SI621_002663"
     CDS             complement(14519..16024)
                     /locus_tag="SI621_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEI4065367.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEVQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(16014..17606)
                     /locus_tag="SI621_002664"
     CDS             complement(16014..17606)
                     /locus_tag="SI621_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEI4065368.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     RFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDAETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(17603..17809)
                     /locus_tag="SI621_002665"
     CDS             complement(17603..17809)
                     /locus_tag="SI621_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4065369.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            complement(17793..19721)
                     /locus_tag="SI621_002666"
     CDS             complement(17793..19721)
                     /locus_tag="SI621_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEI4065370.1"
                     /translation="MISDAQKAANAAGAIATGLLSLIIPVPLTTVQWANKHYYLPKES
                     SYTPGRWETLPFQVGIMNCMGNDLIRTVNLIKSARVGYTKMLLGVEAYFIEHKARNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPALLELAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            complement(19693..20241)
                     /locus_tag="SI621_002667"
     CDS             complement(19693..20241)
                     /locus_tag="SI621_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEI4065371.1"
                     /translation="MKVNKKRLAEIFNVDPRTIERWQSQGLPCVSKGSKGIESVFDTA
                     MAIQWYAQRETDIENEKLRKELADLRAAAESDLQPGTIDYERYRLTKAQADAQELKNA
                     REDGVVLETELFTFILQRVAQEISGILVRVPLTLQRKYPDISPSHLDVVKTEIAKASN
                     VAAKAGENVDGWIDDFRRTEGS"
BASE COUNT         4377 a         6402 c         4920 g         4711 t
ORIGIN      
        1 tggtttctga ttttttggct gctgtcgcgg agtttgccga tgcagtctgc gaggacgctg
       61 ccgcctgtgc gctgttagct gcattcgttt ctgaggtttt cgccgcgttc ttcgatgatg
      121 ccgctgcagt ttcggatttc tttgccgccg ctgcgctctg agaggcggct tcggcgttgc
      181 gtgccgcttc ttccaccatc gcctcaaaac gacgcaatgc ctccggcatg acatcatctt
      241 ccgtcatggc accgagaaaa tcattcagcg tacctggtct ggagccttca tagacggtaa
      301 tggtcccggc atgtgaaggc agaaaacctt caaccagcag gatgacgctg tactgaccat
      361 actcaacgtc catgctgtaa cgcccggctt catccggatt ttcagaggcc accgtgttca
      421 ccagtaccgt ggtgctgtta cgctttgcct tcagttgaat agtgcagttc tgtattggtt
      481 ttcccgcacc atctttcagc acacctgaga tttttactgc tgccatatcc actccacaaa
      541 aaagcccgcc tgaaccggcg ggctgtcata acactgtgtt acctggctaa tcagaattta
      601 taaccgacac ccacgatgaa accgtcagtg cgccagtcac cactgccgga gccttcataa
      661 gcaatatcaa tggccacgga ttcggtcggg ttaaactgca cgccagcccc ccacgccaga
      721 gacgtgttgc tgtggcgacc gtcatcactt ccggtcagca catcgtgcgt tttccccttg
      781 atgtcagtta cgcggagata atccccggaa aaagtcgaaa cacggctgta agccacacct
      841 gccatcgcat aagcactgaa ccattcattc acgcgtacag acggccccgc catcatgctg
      901 aaccagcggt tacgcactga atcttcatgc cagcgggtat cgctgtaatg cgttttttgc
      961 tcatctttgg cattggcata actgaatgac gtcaccagcc ccagcgtgtc cgtaaattca
     1021 taacggtatt tcacgttaat gcccttcagg tcatcgttgc cgggcatatc attatgggtc
     1081 tgaagatacc cggcgcttag tgtggactga tgctctgctg cgctcgctgg cgtaccagcg
     1141 acgaccagcc agactactgt ggacagaata acagcacata atttacgcat aattacctct
     1201 cgcttttctg caataaaaaa ggcgccattt ctggcgcccg tatctgggtt ataaaattca
     1261 gctaatcgtg atgcctgcag tggctttctt catcacaaca accagcaaat cgctgatact
     1321 tgctgtggga taccagccat ttaccagcca tgctgacacc gaaaactcca gcgtcatgtg
     1381 accgtgaccg gcagacatat caataacgcc actgtaaatc agcgtattat ccagcgcggt
     1441 acggttataa atttcagcac cgtttttccg cactatcaga cggcatgagg agtaaatatc
     1501 agtatgctct ctctcatgtt tagcgccgct gaatgccacc gccggaataa caatctgccg
     1561 gtcaaacggc tgatcgtcat aaatcctgac ggtaatggtc cctgatggcc accgttccgg
     1621 tgcacgggag tcccggggga aagctttgcc cactgtttta acgagatcgc cttcaatctg
     1681 gttcgcggac agttttccca gaacccggca gttctcgtta atcgtgacgt tgttgagcgt
     1741 cccggagttc gcattcacgt taccgctgat atcggcattt ttcgccgtca gccgcccgtc
     1801 cggtgtcagg gaaaatgccg gaggattacc gccgctggta atggtgggag ccgtcagata
     1861 tttcaggaac acttcgttca tgaatatctg atcgccctga ccaacaaaca tcggctttgt
     1921 gttgccattc gcaggattaa tcatcgcaat cctgtccgcc gccagcagca cctgactctg
     1981 cataccgtca ggagtattct caataccggc accaataccc gcgatataaa ggcgtccgtc
     2041 ctgcatctgc tgcagcttca cagcccacat gctgttcagg ttattatttg tatcaacctg
     2101 aaccttctgt atctgctgga tcgctgcact ctggtcttcc agtttcttat tgacggtctg
     2161 cgtgatttca ttgctgacat ccgtaatgga cgtcctgatt tcagccaggt caggcgcaag
     2221 ctgaccgtta tcaatctgcg cccacaactc ctgagccaga tgggttttcc ctatctctcc
     2281 tttgaaaaaa tccagatagc cggatgcatc atcactcggc tggccaacag cctccacaaa
     2341 tgccgatttg ccaacggtgt tcacactgcg aatgtaaaaa taataatcat ggcccggttt
     2401 gatattgata ctggcggcta tccagtacag cgccgtacca agatagcggg ctgtggtttc
     2461 aacctgcctg atatcggtaa tccgcttttc cgagaaccag aactcaaact gtaccgtcgg
     2521 atcataaatc gcaagatgcg gcgtggcggt tatctgaaaa tagcccggcg tcagctcaat
     2581 ccgcgacggt gctgccggtg cggcaatccg gaacgatacc gatgccggat cgccctgctg
     2641 tccccgggta tttaccgccc ggaccgtcag cgtgtaacgc cccagcgcca gttgcgtgaa
     2701 gcggtatgtg gtttccgtcg tccgggccgt gctgaccagc cgctcactgc cgtcgtctgc
     2761 tgccacagtc aggcgaagca taaagctcac acccttcacc accttcggcg tgtcccatcg
     2821 cgccagcacc tgatattccc cgctgtctgc ggtgacttcg gcggtcagat gctgcactgc
     2881 tggcggcgtg acaccattca ccgtgccgct ctggtcaccg tcaaagtgcg ccccgttatc
     2941 cacgatggct tctttctccg gtacatgctg cacggcagtg atggcatacg tgccgtcgtc
     3001 gttctcacgg atactcacac agcggaacag gcgctggcgt agcgtcggca gcttcagccc
     3061 ccacacgctg tattcagcaa caccatcagg aacacggctc actttcacct tcacgccgtc
     3121 ggtgacggac tgaacctcca cgctgaccgg attcccactt ccgtcaacca ggcttatcag
     3181 tgtggtaccg gaggatggca gcgtgatttc acggtcgagc gtcagcgtcc gggtctggct
     3241 gttcaccgcc agcacgcgac caccggtgct gataccggca tagtcatcat cacagatttc
     3301 aataacatca cccggcacat ggcgaagccc ttcggcaccc acgctgaaat ccacggtctg
     3361 cgtctccagc agctccgttt taatcagcca cagcccggcg cggtgtgcct gcccccggct
     3421 ggtacagcca aaggcatcca tcttcgtgac gttacgaccg taacggagaa tggcctgcgt
     3481 gtcctccaca agctctgtcg ccgtctccca gccgttatcc gggtcaatcc agttcacctc
     3541 aacggcatta tggcggtcct tcagggcgct gaagctgtag cggaacggcg cgccatcatc
     3601 cggcatcacc acattactgc ggttataggt ccacacctta tctgatggtc ggtcctgcac
     3661 gaacgtcagc gtctgcccgt tccataccgg catacagcgc atcgccgagc agaaatcact
     3721 gagcacatcc cacgccttgc gctgtgtggt caggtacgca ttacaggtaa tgcgcggctc
     3781 cgtgccgcca aagccgtccg gcaccgactg gtcgcagtac tggccgatga catacagcgc
     3841 ccatttatcc acatccgccg caccaagacg tttccccatg ccgtagcgcg gatgggtcag
     3901 catatcccac agacaccagg ccatgttgtt gctgtatgcc ggtttaaacg ttccgtccca
     3961 gataccgctg tattgtcgcg tctgcgggtt atagttcgac ggcacctgca gaatgcgccc
     4021 gcgaagatga taattacgac tcacctgctg gctgccgaac tgctccgagt ccacctgtac
     4081 gccgaccagt gccgtgttcg ggtagcactg tttcacatcg atgatttcgg tgtatgacga
     4141 ccagagcgtt ttgttctgca gctggtctgt agtgctgtcc ggcgtcatcc tgcgcatccg
     4201 gatattaaac gggcgcggcg gcaggttatc caccaccacc gagaccagat actgtgaggt
     4261 ggttttgccc ttaatggtga tgtctttttc cgtcacccag ccaccattac gctgtatctg
     4321 aaccagcagg cggacttccg acggattcct gtcacccttt gaggtggttt ccaccagtgc
     4381 ctgcacaccg aaggtaaagc gcagacggtc gatgtttgcc gacgtgatgg tgcgggtgat
     4441 cggcgtgtcg tatttcactt ccgtacccag caccgtctcg gagccggagg attcaaaccc
     4501 ctccggcggt gtctgctcct gctcacctgc ccggaacacc accgtgacgc cggagatatt
     4561 ggtattcccc tcactgtcca gcactggcgt actgttcagc agcacacttt ttaatccgtc
     4621 caccggacct tcaaccggcc cttcactgat ggcgtctatc acgctcagca tctgggatga
     4681 tttcaggttg tccttcgctt cgcgcggggt atgcccctta ctgctgcctt tacccattcg
     4741 tcatgatcca taaacgataa aaccgcccgg aggcggtttc acataaaaca ttttgcatca
     4801 gcgaccaatc accacaacct gaccaccatc cccttcgtct gccgtgctga tctcctgaga
     4861 aatcacgcgt gaccccacgc gcatttcacc gtacagaaca ggcagaacat tgccctgggc
     4921 aaccatgtta tccagtgagg agaaataggt gttctgctta ccgttatccg ttgtctgtgt
     4981 acggggagtt ctggctttcg gtgccagcat ctgcgccaca ccaccgagca ccatactggc
     5041 accgagagaa aacaggatgc cggtcatacc accggcccca atggctgccc cccatgctgc
     5101 aagggtggct ccggcggtaa agaatgatcc ggcaatggcg gcagccccca ggacaatctg
     5161 gaatacacca cctgacttgg ccccggcgac tctgggaaca atatgaatca cagcgccatc
     5221 aggcagagtc tcatgtaact gcgccgttaa tccggacgtg ctgacgtccc gcccggcaat
     5281 ccgtacctga taccagccgt tgctcagttt ctgacgaaac gccgggagct gtgtggccag
     5341 tgcgcggatg gcttcagccc ccgttttcac acgaaggtcg atgcggcggc caaatcgttg
     5401 caaatccccg taaaggcaga tgcgcgccat gcccggtgac gccagaggga gtgtgtgcgt
     5461 cgctgccatt tgtcggtata cctctctcgt ttactcagtt gttcaggaat atggtgcagc
     5521 atctcgccgt caccacagta aatagcggca tgattcggca ccgatgaacc aaagcagcac
     5581 agcagcacat cgcccggctg cgcctctgtc agtgcgacac ggtaaaaacc agtcgcctcc
     5641 atattgtcaa gatagagatt ctgagcgtga cgccaccagt catccccgcg atgaaaatcc
     5701 ggcatctcaa ttcccgccag atggtaagca tcccggaaca gcgtgtaaca gtccgtcacc
     5761 ccgtgctcaa agcaccgccc ggtaagatgc ggcacacagc ggaacttgtg aatcgccccc
     5821 cggcagacca gccaccacgg caaatcactc tgcacctgca gccgccggtc ggccttactc
     5881 agccagggca gaccaccggg gtgactgtgg accagcgcca caatctcacc ctgcatttct
     5941 gcctgcagcc agtcctccgg agccatccgg aaataatcct ccggctcacc ggaaatattc
     6001 acgcagggaa aatatctttc cccctccggc gctctcacca cgaagccgca cgactccgct
     6061 ggcgcacatc gccgggcgtg cgccagaatc gctgattctg tctctgtcat gggattactg
     6121 cgaaagtttg ttaatggaaa ggaagccgcc aaagttaccg acgttattgc gaaacgtaca
     6181 gccgctcagg catttgctgc atttatcctt cgtgatatcg gacgtcggct ggtcatattc
     6241 atccgcgacc gccggaccgc tataaccgca ctcatcgccg cgataggtcc aggtgcaggt
     6301 gttggccagc atgatacgcc ccggaaaaac agagccatcc gtttccgtcg gtgtggacag
     6361 tacaaaagag gcactgaccg cgctcagttc gctgcactgc tcaatgcgcc agcggctgat
     6421 cacctcctgc tccggatcgg cgtctctgtt tccgttgacg aagttcaccg catccagaaa
     6481 acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca gatcttccgc
     6541 catcccggtg accataccgt acaggttaga aaccgtcagc gtgggccgcg tactggtgcc
     6601 tttgccattc agttcaaaac cgctcccctg aatgggatac gcctgatact gcctcccctg
     6661 ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac gttctccacc
     6721 gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg cacgggtgca
     6781 ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttc aaactctgcg
     6841 ctgaactcaa cacgcagcat actgacccgc gacgaccatt ttgcgcaggt cacctttatc
     6901 tgccgccact cataaggcgg cgtccacaga aaggctttcc agcccccgtg ctcttccaga
     6961 aacgactcca gtaccgtggc ctcctcacgg gggacagaaa gcgtcacgct gtacgttttc
     7021 aggttggcat tcagcccggc aggcgctcgc tgggaatagc catcaccaaa gcgcaccttt
     7081 cttacagaag gggccgaagc cacatccata ccgggtttca ctttccagcg gaaggttttc
     7141 atcgtccacc tccggagaac agaccaccat cacgcatctg cccggtcaca acatccattg
     7201 ccgccttacg ggctacgtca taaaccgcct tcagcgcctg tggccctatc tgaccgttcg
     7261 tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cttcccggac gcctgcgacc
     7321 gactgtccgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag ccgcgcatca
     7381 gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtaaag acaaactcac
     7441 cacggtgaac aatccccgct ggctcatatt tgccgccggt tcccgtaaat cctccggttg
     7501 caaaatggaa tttcgccgca gcggcctgaa tggctgtacc gcctgacgcg gatgcgccgc
     7561 caccaacagc cccgccaatg gcgctgccga tactcccgac aatccccacc attgcctgct
     7621 taagcagaat ttctgtcatc atggacagca cggaacgggt gaagctgcgc cagttctgct
     7681 cactgccggt cagcatcgcc gccatattct gtgcaatacc atcaaaggtc tgcgtggctg
     7741 cactttttac ctgcgacata ctgtccgtgg cgctctcttc ccactcactc cagccggact
     7801 tcaggcctgc catccagctc ccgcgaagct ggtcttcagc cgcccaggtc tttttctgct
     7861 ctgacatgac gttattcagc gccagcggat tatcgccata ctgttccttc aggcgctgtt
     7921 ccgtggcttc ccgttctgcc tgccggtcag tcagcccccg gcttttcgca tcaatggcgg
     7981 cccgttttgc ccgttgctgc tgtgcgaatt tatccgcctg ctgcgccagc gcgttcaggc
     8041 gctcctgata cgtaaccttg tcgccaagtg cagccagctg gcgtttgtac tccagcgtct
     8101 catctttatg cgccagcagg gatttctcct gtgcagacag ctggcgacgt tgcgccgcct
     8161 cctccagtac cgcgaactga ctctccgcct tccacaaatc ccggcgctgc tggctgattt
     8221 tctcatttgc tccggcatgc ttctccagcg tccggagttc tgcctgaagc gtcagcaggg
     8281 cagcatgagc actgtcttcc tgacgatcgc ccgcagaccc cctcacgccg gactgtttcg
     8341 gctttttcag cgccgattca taatcctttt tcgccgctgc catcagcgtg ttgtaatctg
     8401 cctgcaggat tttcccgtct ttcagtgcct tgttcagttc ttcctgacgg gcggtatatt
     8461 tctccagcgg cgtctgcagc cgttcgtaag ccttctgcgc ctcttcggta tatttcagcc
     8521 gtgacgcttc ggtatcgctc tgctgctgcg catttttgtc ctgttgactc tgctgttcag
     8581 ccttctttct ggcggcttca agcgcaagac gggccttttc acgatcatcc cagtaacgcg
     8641 cccgcgcttc atcgttaaca aaataatcat ccttgcgcag attccagatg tcgtccgctt
     8701 tcttaaacgc agcctctgcc ttaatcagca tctcctgagc ggtatcagga cgaccaatat
     8761 ccagtaccgc atcccacatg gatttgaatg cccgtgctgt cctgtctgcc caggtctcca
     8821 gcgtgcccat gttctctttc aggcggcggg tctggtcatc aaaccctttc gttgcggcct
     8881 cgttcgctgc ctgcaatgcc ccggcttcat cgccggaacg ctgcaactga gcaacatacg
     8941 caatctgctc cgccgtcacg ttatggaact gacgcgccat cgctgtcagc cccgacgtcg
     9001 ggtctgtggt cagcttcccg aaggcttcag cgaccttgtc cacctccacg ccggatgcag
     9061 aggagaaacg cgccacactc tggctgatgg atgcaatctg agcctcaccg cttactcccg
     9121 ccttaaccag tgcgctgagt gactcgctgg tctggttaaa cgtcagccct gccgcctgcc
     9181 cggctctgga caggaccagc atacgatctg ccgtcagacc cgactgattg ccggaaagga
     9241 ccagcgtttt gttgaaatcg gacagggttg agttgccctg ataccaggca tacgccagcg
     9301 caccggtcgc caccgccagc gaggtggccc cgaccatcgg cagggtgatc gcaccggcaa
     9361 gccccctgaa catggggatc atcccaccga aggagtcctt cacctgacca ccctgttgca
     9421 gcaggatcag ccacggactt tgcccgcctg caagctgcgt ggccacgtcg gtgaactgtg
     9481 caggcagcat acgcatggcg gctttatact gcccgacgga aatccccgct ttctgtgcag
     9541 ccagtgcctg tcggctcagc gactgttcaa cgactgccgc tgtttttttc gcatcacttt
     9601 ccgtaccgga aaaatgacgc ctgactctgg ccatctgctc gtcaaatctg gccgcatcca
     9661 gacttaaatc aacgaccaga tcgcctaccg gttcagccat accggactcc tcctgcgatc
     9721 ccttctgata ctgtcatcag cattacgtca tcctccgtca tgtccgccat atccggggaa
     9781 gcggggataa cttcattccc gtccgggcca aaacgaacgc ctccggcaag ccctgccgct
     9841 ttctgcatca gcacatcatc ttcaggctct tcgtcagcct cgcgccggtt cagcagactg
     9901 aaatccagcg gatgcatatc cggatcgctg aaaaacaggc tgagcacggt gtacgtcagc
     9961 ccggaaaagt gcatatccag cagaacatca tgaaaataat gggtactgta aaagcggtgc
    10021 cagtcggcat actccgtgga tgacatcccg gcaagcatgg cgcgccagtc gggtcgcccc
    10081 atttcacgcg ccagtttcag ggcaaaactc agctcaccgt cgaacacttt cccgcagaaa
    10141 caggctctgc aggcccggcg tcatctgcct gttcaggggc attattcaca acaaactcag
    10201 acattccgga cagacgtaac accacgtttt cagcctgagc aattgcctcc gtgggccagg
    10261 tggtaagcac ttcctgctca atctgcgtaa tggcttcatt catggacggc agctttgtct
    10321 tctgcggatg gttatgccac agagacatcg ccaccagaaa cgccccgcct ctgataagat
    10381 cctctacaga cacctgcagg ttgccactgg attcagcctt tttttcctgc tctttcaacc
    10441 aggcaagatg ctcaatacgc tgcagggctg acagttcaga aagcgtgacg gtcacaccgt
    10501 tatgttcaaa tgattcggtt ttcaggaaca tcgctgactc tccggattaa ctggcggtga
    10561 cgttgatttc tgcaaccgca gcaagttcac cattaccgga tacaaccgga atgttgacct
    10621 tacctgcagc gacgcctttc acggtgatgg tcataccact gaccgacacg gtggcttttg
    10681 ttttatccgc tgacaccgca cggaagctct tgtcggttgc gccctccggc tggaaggcca
    10741 cggtcagcgt ggtgctctgc cctttcacca ccgaggtgct ggcaggcgtc acggtcatgc
    10801 cagttgccgc tgttaccgtg ctgcgatctt ctgccatcga cggacgtccc acattggtga
    10861 ctttcaccgt gcgggtgatc acttccttcg ccgtcaccgc cttaccgata ctgctgaccc
    10921 agccacggaa cacatcgacc gtgccgttcg ggaagcggat tttataggca cgggtatccc
    10981 cttcattaaa ccacgccagc agcgcctgct gcccctgctc tccgggcatc cacgccagcg
    11041 tgaagctggt atctccggcg gatttctgcc cctgcccggt cgcagtccag tccgcatctt
    11101 catcatcgag atagctgtcg tcataggact cagcggtcag ttcgccgggc gtcaggtctt
    11161 taacttttgc cagacgcgac cagtcattgt ctgaaagcgg gtttgcataa gggtcaccgc
    11221 tccccttata aacccacagg gtggtcccgg cacctttcac cggcattact ggatttggta
    11281 caggcatatc gtcctcacat ttcataggta atgacataag tcagatcggc tgaactccac
    11341 aggcccgcat catcgtcgcg ccggtagtca tagccactgg ccaccatact ggtgatcaaa
    11401 tctgacagtg ccgggatatc gctcatcacc ggataaatcc gggactccat ccacgaatcc
    11461 agctctgaat ccggcacctg agcaggcagg aaaacttcaa tatgcagctc cgcctgccag
    11521 gtatcgctgt ccagctcttc gcccgtgtat tcagcgcggg tgagataaac ggcaattgcc
    11581 ggaaaatccg cctcatcaaa aacagcgggg cgaccatcaa aaagcgtcgc cccggtgtca
    11641 tgtttttcca gtgcatccag tacggctgca cggagttcag tatgtttcat cgctttatta
    11701 ccatcctcag ttgatgctgc agcgcatagc ccagctcttt cggaagacgc tcacgccgta
    11761 tccgctcaat attctgttta aacgccgtgg tcagcggcac cgccatcgga attttcacca
    11821 catcaatggg gtaacggttt ttcccggcca cacgctgcat gacatgccgc cggccatttt
    11881 tcagttgctg aataaacgcg ccgggaatac gacggttacc caccacaagc acgctgccgc
    11941 cacctttcag ggatgaacgc tgcccctttt tacgacgcct gcggcgggac aggataaccc
    12001 gcgcattacc cagcctgatt acgggcaaat ccccccggtt aaccttgatt ctggcctgcg
    12061 gatttttgac cgtggccctt ttcagcctga ccctttcctt taccagtttc cggcgtacct
    12121 ttgtctcacg ggcaacctgt gacgccgact gcgatatcgc ggatgaagca acgcggttaa
    12181 tggccattgc ggcggcacca ggcaccgccg ttttgctgat acggctgagg ttttcaacgg
    12241 cctgctcaag accctgtatg gccatacatc cccctttcag cgacgacggc tggcggctgg
    12301 tggttgcccc cggttgagcc agagataaca actccctccg tcatcaggag aaacacggtc
    12361 cacccagaac atctcgccgt taatggtcag cgtgtcacca cgccgtacag cacaaaccgt
    12421 atccgtaagc acaaataatg acgggttact tccttcaata cggatcccac tactggcaaa
    12481 acccagcgac tccggatcgt caaaaacgcc atgaacctcg ccgccacgct gtgcaccgga
    12541 ggtgaactgc gcactgatgc ccatcacttc aacaatcgta ctgtccaccc cggcaagggc
    12601 ggcatcaaag gcattctgaa aatcacgcat aaacagccat tccaccatca acgtgtgttt
    12661 ttgcatctga ggacataatc agaatcaccc gaccaacatc cgcaagctca acggattccc
    12721 ccgtttcacc atcaacgcca cagagatgga ggcaggtcag aactctgatg cgcgttaacg
    12781 cgccggatgt ttcctcacga acatcatgag ccgcggtttc ccgctcccgg atatccatat
    12841 tcataacctg tacatcatcg ccggatgact gcatttcttc ttcccattct gccacccgct
    12901 gcgctatctc tgcggcactc ccggatatat ccggctcacg ccccagaatc agggccagtt
    12961 catcaagccg tttcagattt tgctctttcg ttgccatatc agccccctgt gaaaaaagac
    13021 acgggggcat ttcgcccccg ctcacggatt atttcacctg taccaccaca aactcatccg
    13081 ggtccggcaa caccatcagc ggcgcggact gcgtcatggt aaattcacgg gcgggatccc
    13141 ccaccgtcag ccagtgtttc ggataacggg aagaggccac cacaccttcg gacaacgcct
    13201 gagcatcctg aatggcaccg taacaacgga tcccatctgc agcagtattc cccagaacca
    13261 gcatgccatc tggaagataa cgtttttcga taccgtcttc tgctatataa gacgttttcg
    13321 ccacaacaat ggccagatcg ccgtaatacc ccttgaagga caccacagcg cccagatctt
    13381 tcactgccgt ttcgagttga gaatttgaac cgcgacgggt atccagtttt tcgcggaaca
    13441 gcttaaaacc attcagaaga cgccagacgg taccgtccat aatggcaata ttcacaagac
    13501 cgctggcctg gtcgcagtag aggtcaatat catgtgtagg atcgaacgtg tcacgatcct
    13561 gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac ctgccaaaat
    13621 cgacctcaat tttctcgaac tggtctcctt ccatggtgta tttgccatac aacacagcat
    13681 ttaccgcctg catttcttcc acctggacaa tcgcgtgctc ttcctgtttg aggttatcgg
    13741 taatgatacg cagacggcgg taagccggat cattcagttg agatggatct tcaccaggaa
    13801 ggcgctcaac cgcctgctgg taattaaatt cgtgtttggg tttgacgtag cccggacgca
    13861 acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc ggggagacat
    13921 aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg gtatggaaat
    13981 tcaccgtacg gcggaaaaac agttccagaa acagcgcacg aaatttaact ttttgttcgg
    14041 tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt tcattcagaa
    14101 atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg tgactcaggg
    14161 ctctgccagc aaaggcgttg gcctttttgt gttcatccac actttcaggc cagtggattg
    14221 cctccgtcgc aaaggttccc gacttgtaat acgtcagcgc cgtctctgtg ccttcaagcg
    14281 gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc accagtttcc
    14341 cggtggcttc atccagcatc aggggcgtca gtgccggtgt tggcgaggaa atcccgctgc
    14401 tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc gcacgctgtt
    14461 cagtgatggt tttcgtcacc atattgtaaa aacctcctat tgatggtcag cactggcttc
    14521 atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc tgtggcacgc
    14581 caccctgtac cgctgccggt gaatggttcg ccatgaaatg ttcaaacatg gcggttgtgg
    14641 atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg gcgctctcca
    14701 cagtcattcc cgggcaggca gccagctgtt cagcctgcac ctcagcccct tttgcctcat
    14761 ccagtgccat gatctgatca cggagtgatg gtccggcatc cgcctgcggt gaagcagcca
    14821 ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt gccgccagtt
    14881 gttcacgacc gttcgcttct tcacacgcca taatgcgatc ggcttcactc tgcgtggatg
    14941 ccaccggctg ctgcggtgcc gccgcggcca gaatcgcccg ggcctgttca acgctcatgc
    15001 cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc tggcatgtca
    15061 ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt aattgcggca
    15121 tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg gcatccgacg
    15181 cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca aaaacagcgg
    15241 cctctgttcc cgtgactgca tcaacagaca gaccggtaaa catcgccact ttttcggcaa
    15301 acatccggcg cgccgcatca atccgctgct gcatgtcctg gcgaacctct gccggcaacg
    15361 cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg atatccacac
    15421 cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca atggaaccga
    15481 tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc atggctgcag
    15541 aacaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg cggtaaatca
    15601 tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg tccagcagta
    15661 cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaata ccgtcatagc
    15721 ctgtcattcc ggaaaatggc cgcataccac ccagccggtg caccagcgtg ccggtcaccg
    15781 gcagtacagc aataccgttc accaccctgt aaacacgggc cggtcgttta cctccggcca
    15841 tgtactcgtc cgtttcagcc agcattccgg gagcatcaaa ctgtacctgc tgttgtggta
    15901 ccgaaagact tgctgccccc atctcgcgcc cgagcgcgca aaagaaaacc cgcgcatagg
    15961 cgggctccag aagcagcggt tcattgaatg ctgcggcaat aatgtgtgaa agattacgtc
    16021 tcacgtggtg ttgtctcctc ttccggccta cgactctccg ctatctgctg ctgatacgcc
    16081 tgcgctatcc acaccggacg tgagagtccg gctttttgcc gctcagcaga ttccctgacc
    16141 tgctggcgga aaatgtcctg ataatcctcg cccatcagcg ccagctcttt ctcatacgtg
    16201 ctcagtccgg cctcaatgcg catcactgat tcctggactt ccttgagccc gtcaatggcc
    16261 attcttccgg caccaatcca ctctgcccgt gaccaggctg atcgcgcctg ataaaaatca
    16321 aaacgcgccc gtggcggacg aataatcccc cgaagaagtg cctcttccag ccagcaggaa
    16381 aacatctgcg tggccagccg ggccgcaata aattttcgcc gccccataaa atagcgccac
    16441 gactcattgg cggatgcgcg ggcacttgaa taactgacct tcgagtaatc acgggacaac
    16501 tgttcgtagg aaacgccaag accggcggcg atataccgca gcagcgcctg ttcaagtgcc
    16561 gaaaatccat tgtctgaatc ctgcgcagtc tgcagtttca gatcatcccc ggggaaaagg
    16621 tgcggaattt tgacaccgcc cagcgtcacg ttattcgtgt cataccagct ggagaacttc
    16681 tccagaatat taataagcgg attatccttc tgcccctgcg gcgcaccggc gatatattca
    16741 aaggcctttt cggtatcaag ttcactttca attgtcgctg catacattgc cttcactatg
    16801 gctgactgaa gctgtgttgc ctgcagggaa tccagcatct tcagccgttc catgacgctg
    16861 taaaactgat tagccccacg ggtctgcccg tcctccaccg gctcgaaaat atgcagcatg
    16921 gccggacgcc cggtgggtag ttcacgcggg atccgttccc atcgtccact accagagaac
    16981 ggaaaatcat cctcacagat atggtacgca acggcacggc catatcgatc gacctccaca
    17041 ccggcccgca gaaagcggtt ccccataccg tgtcctggcg tgtccacccg tttcggactc
    17101 acggctttaa aacgcgtacg gaataactgc gtggtctccg catcccagac cggctgcaca
    17161 aagatttcgc cgttaaacgc atgaacgccc acaccttcac ggataaattc cgtaaacgtg
    17221 cgtttccctt ccacgtcgat ctcgccaaac atcccttctg cgtattccga ccaggccgcc
    17281 tccacctcat cgacaaaacg ttttgccgcg gtctcccgca tccccagcca gcgccagttc
    17341 ggacggtagc tgataagaaa catatgcccg acaatgtgat ccttatgcag tgccaccgca
    17401 ttggccgcta tcccgttatt gcgtaccaga tcatctgccc gggcattccc cagacgcaac
    17461 gcgggcaaca gagctgcatc ggcactctgc gccggtggca accactccgc catttgcccg
    17521 ccaaatcctg cgccgccccc gttgtagctg aggctctccc gaagcggaac gccgttcaca
    17581 tcaatcagga caggcgttcg tttcataacc tcactcccag cggacgacgg cgacggcggg
    17641 ttgtccccag taccgactcc gcatcattga tcgcccggtt aagctcatcc agagaagccg
    17701 ccgtatattc aattctgcga ccatctttct ggacagacac cacccgttta ccggttaata
    17761 aatcaaggcg cgcctgacgc agcgcctgca gttcagcgac tgtaaccatt cactcctccg
    17821 gacagcttcg ctgccagttc tttaagggtt ggccgggtcg tctcttcttc ccgggatttt
    17881 gccagtacag ccagatcaag ctgccagcgt tgcacggaca cacgtaatgc cgcgtaggca
    17941 tacaccaggc agtccagcgc ttcgttacgc cgctttttgt tatcccacag cagacgcatc
    18001 tttccttttt cccacttctc cacaagctct tccgccacca gttgctgcgc ctctgtctgc
    18061 gaaaaaatct ccggatcatc aggaaaacgg atggcataag acgtggcttc atccgcaggc
    18121 gtgggatcgg ctttcatacg ggcatagaga atttcttttg cggtgtccgt ccccacttca
    18181 cacagataca cgccccgctg attgcgggtt tttggcatgg tgatcaccgg cttgccatag
    18241 acagatgcgc cttttaccgg cagcacccgg aaaacaccgt gtttttttga cctctgataa
    18301 acgatttcgc catcgatccc cccgatgtcc cagcagacac gggaaatggt catttcggtt
    18361 ccgtctgcat ggcggtattt tttgttgatc gccgcatcca cacgtaacag cgtctcttcc
    18421 tcatcgggac gtcccataat gatgatttta tccaccagaa aggcttcctc tcccggagcc
    18481 catccccaga catacatctc aaaacggttt cgctgcgagt caatgcccgc cgtcagataa
    18541 accacccggg ctggcaccgc cgccgtgtaa tgaacaactt tatccatcag tacctggtga
    18601 tcgagttttt cgcccacggc ctcttcccag gtctcgccca gcgtggtgtt cacaaaagtt
    18661 ttcaggccgt tgggatcttt cagtgcatcc agccagtcat agacaatctg tacccaggtg
    18721 gtgaacggac tgtacgccgt ccagatatgg aaagtgatgg agcgcggcgg cggaatttca
    18781 tcaccccggg cgctgaaaaa catcaggccg tcacgggtcc acatgcccgt gttttcacag
    18841 atccaccgcc cgttactctg gtcaagctca gactgatgga tcacacagcc atgatgctca
    18901 caaaggtaga aaacactttc tggcttattc ttctcccact taagaccgaa aggcgaggca
    18961 tcatcgccaa atttcagata ctgctcctcc ccacagtgcg gacagggcac ataaaaacgc
    19021 atgaaatgtg ccgactcgtt agcggctttt tcgatctggc aggaaccttt gattttaggc
    19081 gtcgagccgc gaatggattt tggccataca gagccctcaa tacgtttatc ccccagcagg
    19141 gttggcgaac cctctttttc aacatccggt tcgaacgagg aaagctcgtc ataacagacc
    19201 acatccacgg atttttcacg gtagtttttt gctgccgcac cacccaaaca ccagaacccc
    19261 acaccggagg aaaaacgctt cagggtgagc gtattatcgc ggtgttttct tccgaaccat
    19321 ggagccagct ccagcaatgc aggaacatcc cttatcgttg gctcaacatg agatttcata
    19381 aaatcttcag cagctgagtc cgtgggctga aaaagaaggc tgttgcgtgc tttatgctca
    19441 ataaaataag cctccactcc cagcaacatc tttgtataac caacacgggc agatttaatc
    19501 aggttaaccg tgcgaatcaa atcgttgccc atacagttca tgatgccaac ctgaaacggc
    19561 agtgtttccc accgccccgg ggtataagac gactctttag gaaggtaata atgtttattg
    19621 gcccactgaa ctgtcgtcag tggaacagga ataatgagag ataaaagccc tgtagctatc
    19681 gcaccggctg cattagctgc cttctgtgcg tctgaaatca tcgatccacc cgtccacgtt
    19741 ttcacctgct ttagctgcaa cattggaggc tttcgcgatt tcagttttca ccacatcaag
    19801 gtgtgatggt gaaatgtccg gatatttacg ctgtaatgtc aacggcacac gcacaagtat
    19861 ccccgaaatc tcctgtgcca cacgttgcag aatgaaggta aacagttcag tttccagcac
    19921 cactccgtct tcacgggcat ttttcagttc ctgcgcatct gcctgcgctt ttgtgagccg
    19981 gtagcgttca tagtcaatgg tgccgggttg taaatctgac tccgcagccg cacgcaaatc
    20041 ggccagttct ttgcggagct tttcgttttc gatatcagtt tccctctgcg cataccactg
    20101 aattgccatg gcagtatcaa atacagattc aatgccctta ctgcctttgg agacacaagg
    20161 gagcccctga gactgccagc gttcaatcgt ccgcgggtcc acgttgaaaa tttcggcaag
    20221 cctcttttta ttaaccttca taaaacaacc cattatcaaa tacaaggccc gacatgaaaa
    20281 cgccagaaaa aggcattttc ggacactttc atgtcgaaca tttatgaatg caatattaaa
    20341 aaaaacaaag agttatattc gagaaatacc gacacgattt tccctaaaaa attttcataa
    20401 atagtgaaaa
//
LOCUS       DARNVM010000081        20338 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.081, whole genome shotgun sequence.
ACCESSION   DARNVM010000081 DARNVM010000000
VERSION     DARNVM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.081"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(14..1678)
                     /gene="glnS"
                     /locus_tag="SI621_002668"
     CDS             complement(14..1678)
                     /gene="glnS"
                     /locus_tag="SI621_002668"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEI4065372.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1881..3827)
                     /gene="nagE"
                     /locus_tag="SI621_002669"
     CDS             complement(1881..3827)
                     /gene="nagE"
                     /locus_tag="SI621_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HEI4065373.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAIPNAVSVAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNENARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4160..4960
                     /gene="nagB"
                     /locus_tag="SI621_002670"
     CDS             4160..4960
                     /gene="nagB"
                     /locus_tag="SI621_002670"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEI4065374.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5020..6168
                     /gene="nagA"
                     /locus_tag="SI621_002671"
     CDS             5020..6168
                     /gene="nagA"
                     /locus_tag="SI621_002671"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEI4065375.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDTALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6177..7397
                     /gene="nagC"
                     /locus_tag="SI621_002672"
                     /gene_synonym="nagR"
     CDS             6177..7397
                     /gene="nagC"
                     /locus_tag="SI621_002672"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HEI4065376.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7445..8197
                     /gene="nagD"
                     /locus_tag="SI621_002673"
     CDS             7445..8197
                     /gene="nagD"
                     /locus_tag="SI621_002673"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HEI4065377.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8454..10118
                     /gene="asnB"
                     /locus_tag="SI621_002674"
     CDS             8454..10118
                     /gene="asnB"
                     /locus_tag="SI621_002674"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HEI4065378.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFFDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDSEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10499..10575
                     /locus_tag="SI621_002675"
     tRNA            10499..10575
                     /locus_tag="SI621_002675"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10533..10535,aa:Met,seq:cat)
     gene            10584..10668
                     /locus_tag="SI621_002676"
     tRNA            10584..10668
                     /locus_tag="SI621_002676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10618..10620,aa:Leu,seq:tag)
     gene            10692..10766
                     /locus_tag="SI621_002677"
     tRNA            10692..10766
                     /locus_tag="SI621_002677"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10724..10726,aa:Gln,seq:ttg)
     gene            10801..10875
                     /locus_tag="SI621_002678"
     tRNA            10801..10875
                     /locus_tag="SI621_002678"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10833..10835,aa:Gln,seq:ttg)
     gene            10891..10967
                     /locus_tag="SI621_002679"
     tRNA            10891..10967
                     /locus_tag="SI621_002679"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10925..10927,aa:Met,seq:cat)
     gene            11016..11090
                     /locus_tag="SI621_002680"
     tRNA            11016..11090
                     /locus_tag="SI621_002680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11048..11050,aa:Gln,seq:ctg)
     gene            11128..11202
                     /locus_tag="SI621_002681"
     tRNA            11128..11202
                     /locus_tag="SI621_002681"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11160..11162,aa:Gln,seq:ctg)
     gene            complement(11356..12531)
                     /gene="ubiF"
                     /locus_tag="SI621_002682"
     CDS             complement(11356..12531)
                     /gene="ubiF"
                     /locus_tag="SI621_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HEI4065379.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAQSATFVV
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PVLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGMLKRQALKYALGL"
     gene            12677..14101
                     /gene="miaB"
                     /locus_tag="SI621_002683"
     CDS             12677..14101
                     /gene="miaB"
                     /locus_tag="SI621_002683"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEI4065380.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14254..15294
                     /locus_tag="SI621_002684"
     CDS             14254..15294
                     /locus_tag="SI621_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEI4065381.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15291..15758
                     /gene="ybeY"
                     /locus_tag="SI621_002685"
     CDS             15291..15758
                     /gene="ybeY"
                     /locus_tag="SI621_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEI4065382.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15848..16726
                     /gene="corC"
                     /locus_tag="SI621_002686"
     CDS             15848..16726
                     /gene="corC"
                     /locus_tag="SI621_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HEI4065383.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16751..18289
                     /gene="lnt"
                     /locus_tag="SI621_002687"
     CDS             16751..18289
                     /gene="lnt"
                     /locus_tag="SI621_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEI4065384.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNVFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLAVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18686..19594
                     /gene="gltI"
                     /locus_tag="SI621_002688"
     CDS             18686..19594
                     /gene="gltI"
                     /locus_tag="SI621_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HEI4065385.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFSDLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19764..>20338
                     /gene="gltJ"
                     /locus_tag="SI621_002689"
     CDS             19764..>20338
                     /gene="gltJ"
                     /locus_tag="SI621_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HEI4065386.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGLWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLV"
BASE COUNT         4981 a         5367 c         5224 g         4766 t
ORIGIN      
        1 gtagaactta aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt
       61 taaataccgg tttttccgcc gtagaatggc ggctgtcgag gcagaaataa ccttcacgct
      121 caaactggaa tgctttaccc gctaccgcgt ctttcagaga cggttcagca aagccctgtt
      181 tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca cctgggttcg
      241 gcacgctgaa cagacgatcg tacaggcgga tttcaaccgg cagcgcatgt gccgcgctca
      301 cccagtggat aacacctttc actttacgac catctgccgg atctttgctt aaggtgtcgg
      361 cgtcataagt acagaagatg gtggtgatat tgccttcggc atctttctcc acgcgttctg
      421 ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga cgcttgtact
      481 gcttgttagc ttcttcgcgg aaatcggcgc ggtcgatcca aatctcaccg ctaaacggca
      541 cctgacggct ccccatttcc ggtttgttcg gatggttcgg catggtaacc atttcgcctt
      601 cgccctggta gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcgggg
      661 cgttttcgtt gagatcttca cggatgcagg attccagcga cgccatttca atggtgttgt
      721 cctgcttggt cacgccgatg cgtttgcaga actcacgaat agaagccgca gtgtaaccac
      781 gacgacgcag accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt
      841 cggtcaccag caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg
      901 agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt gtccagcacc cagtcgtaca
      961 ggcgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagcgcat
     1021 cgctgatgca atgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt
     1081 ggtgttcagc aaatttaatg cggtacagca ccggatcgcg cattacgata aatggcgacg
     1141 ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gtacgcattt
     1201 tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg
     1261 gctgcgtcag ggtgccgcgg tattcgcgga tctgttccgg tgtcagttca tcaacgtacg
     1321 ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg
     1381 agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgact
     1441 caacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc
     1501 ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca
     1561 gatagccatt cggctccggc gggaaacggg tgtgtactgt ggtgtgctta ccactggcca
     1621 gatcttcatc gatgatctga cgaataaagt tagtcgggcg ggcttctgcc tcactcatcg
     1681 tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct
     1741 gacaactgtt agttagtgaa aatcgaaaat ctcactgaga ttatggggat gattgatgaa
     1801 aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt
     1861 cgcatcaggt ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccacg
     1921 acatggccct gagctttaat gatcaagccg ctgaaatcgt cgatattgct gcaaaccacc
     1981 gggctaatca tcgagcgggc gttttcgttc agataatcca gatccatttc cagaatcggt
     2041 tgccctgcgc ttacttgcgc gccctcttcc accagacgtt taaagccttt accttccagc
     2101 gctacggtgt cgatacccat atggacgacg atctccgcgc ctttttcggt ttccaggcag
     2161 aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt
     2221 ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca
     2281 ggaacctgat ccagtgccac aacatcaccg gtaatcggcg ataccagctc cgcgacagac
     2341 accgcgtttg gtatagcctg cggttttgct acaggcgcgg cagttgcagg cgcagtttca
     2401 gctgacgcag cggctaccgg accacgggca acaactttct tcattgcatc gccaatggat
     2461 tccgctttcg cgccaacaat cacctgaata gtctgtttgt tcagtttcac taccccagaa
     2521 gcacccagac gtttacacat cgcatcgttg acgcgagccg agtcaaccac ggtcaggcgc
     2581 agacgagtaa tacaggcgtc aattgctttc aggttgtcag taccgccaac cgcagcaata
     2641 tagttggtcg ccagttgagt cagaccttct tccgtgttgc tgttggcttc ttcagtaacg
     2701 atctcgtctt ctttatcttc acggcccggc gttttcaggt tgaacatacg gataaccaaa
     2761 ctgaacacca cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg
     2821 ttctggctgg cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaag
     2881 gagaagcccg cgtggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt
     2941 gcatgcagga ggtacagcag cggtgcaagg aacatgaaca ggaactccag cggctcagtc
     3001 acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga
     3061 cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg
     3121 atcgggaaga agccggacat gaacatcccc gcggtgccgt caccggcata gaagcggttg
     3181 atgtcaccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg
     3241 atggtgttca gtacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag
     3301 ataccggaac ccagcgcgcc cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc
     3361 tgtaccggcg gccagacgta accaaaaatg gccgccagca ccaggcagaa gaagccggtg
     3421 gcaatcggca caaagcgttt gccgccgaag aagctcagga agtcaggcag tttaatatcg
     3481 gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc
     3541 atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca
     3601 cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg
     3661 aagattaatg cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta
     3721 agtaaatctg gctgaccgaa tcgcagcaat agcgccgcca ccggcagcac cgcgataggg
     3781 agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta
     3841 cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc
     3901 gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt
     3961 atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc
     4021 ccttttactg gctaaaccag aaaacttatt ttatcattca aaaaatcagg tcggattgac
     4081 gcctgtctgc gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt
     4141 ttacttattg aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa
     4201 atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ctgatcgtcc
     4261 gtttgttctg ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga
     4321 aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata
     4381 tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt
     4441 cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat
     4501 cgacgccgag tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt
     4561 tatgggcggt gtaggaaacg acggtcatat tgcatttaac gaaccggcgt cctctctggc
     4621 ttctcgtact cgtatcaaaa ccttgactca tgacactcgc gtcgcaaact ctcgtttctt
     4681 tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct
     4741 ggatgccgaa gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc
     4801 cgcagttgaa ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa
     4861 agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata
     4921 tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa tagttatccc tgccctggca
     4981 ccttgctcag ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc
     5041 cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg
     5101 attaaaagcg tctgtccggt agcggaactg ccgccagaga ttgaacaacg ttcactgaac
     5161 ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtgcag
     5221 tttaacgata ccgcagaggc ggtcagcgta gaaacgctgg aaatcatgca gaaagccaat
     5281 gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg
     5341 aaacagggcg tgcgcgttat gcgcgagtac ctggcaaaac atccgaatca agcgttaggt
     5401 ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt
     5461 gtgcgtaagc ctgataccgc gctggtcgat ttcctgtgtg agaacgccga cgtgattacc
     5521 aaagtgaccc tggcaccgga aatggttcct gcagaagtga tcagcaaact ggcaaatgcc
     5581 gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcaaa agccggtttc
     5641 cgcgcgggga ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt
     5701 gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct
     5761 gatggcctgc atgttgatta cgccaacatt cgcaacgcta aacgtctgaa aggcgacaaa
     5821 ctgtgtctgg ttactgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt
     5881 gcgggtaaaa caatatacta ccgtaatgga ctttgtgtgg atgagaacgg tacgttaagc
     5941 ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca
     6001 ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa
     6061 cgtctcggca cactcgccgc aggtaaagta gccaacctga ctgcattcac acctgatttt
     6121 aaaatcacca ggaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga
     6181 caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg
     6241 cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa
     6301 gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga
     6361 ttaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg
     6421 aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accataactc
     6481 tgtttgatct cagtagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc
     6541 agcagacgct ggaacatgcc ctgctgaatg ccattgctca gtttattgat agctaccagc
     6601 gcaagctgcg cgagctgatc gccatttccg tgatcctgcc agggcttgtt gacccggaca
     6661 gcggcaaaat tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagctc
     6721 tggaagaacg ttttaaagtg acctgtttcg tcggtcacga tatccgtagt ctggcgctgg
     6781 cggagcacta cttcggtgca agtcaggatt gcgaagactc cattttagtg cgtgtccatc
     6841 gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggcaacg
     6901 tcggtgaaat tggccatatc caggtcgaac ctctgggtga acgctgccac tgtggcaact
     6961 ttggctgcct ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt
     7021 taaagcaggg ctaccagagc cgtgtgccac tggacgactg caccataaaa actatctgca
     7081 aagctgcgaa caaaggcgat agcctggcgt cggaagtgat tgagtatgtc ggtcgtcatc
     7141 tgggcaaaac catcgccatt gccatcaacc tgtttaaccc gcaaaaaatt gttattgccg
     7201 gagaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc
     7261 aggcgctgaa ggcgtttcgc actaatctgc cggtggtacg ttctgagctg gatcaccgct
     7321 cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc
     7381 atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta
     7441 gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt
     7501 cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt
     7561 gttgctgacc aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc
     7621 gggtgtcgat gtgccggaca gcgtgtttta tacctccgcg atggcaaccg ccgatttcct
     7681 gcgtcgccag gaaggcaaga aagcatatgt ggtgggcgaa ggcgcactga ttcatgaact
     7741 gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat tttgtgattg ttggcgaaac
     7801 gcgttcctac aactgggaca tgatgcataa agcagcctat ttcgtcgcta acggtgcacg
     7861 ctttatcgcc accaacccgg acacccacgg gcgcggtttt tatccggcct gtggcgcgct
     7921 atgcgcgggg atcgaaaaaa tctccggacg taaaccgttc tatgttggta agccgagtcc
     7981 gtggatcatc cgcgcagcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt
     8041 cggcgataac ctgcgtaccg atattctggc gggcttccag gccggtctgg agacgattct
     8101 ggtactctcc ggtgtttcgt cgctcgacga tatcgacagt atgccattcc gccccagctg
     8161 gatttaccca tcggtcgccg aaatcgacgt tatctgaata gcaaccacgc ctcagggcgt
     8221 ggtttcttat ttttacccat cactaaaatt cgcccattca ataaaacccg ccaaaaatta
     8281 tcgattcatc aataaataca cctctacacc ctaccctttt tcattaatca gcaatcacca
     8341 ttacgttttt tatttacccc ctgccaaatc gcttgcgcac agtgctgctt tgcggcattt
     8401 ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt
     8461 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgcaag aaagcactcg
     8521 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
     8581 acgccattct cgcccacgaa cgtctgtcca ttgttgacgt taacgcgggg gcgcaacctc
     8641 tctacaacca acaaaaaacc cacgtgctgg cagtaaacgg tgaaatctac aaccaccagg
     8701 cattgcgcgc tgaatatggc gatcgttacc agttccagac cgggtctgac tgtgaagtga
     8761 tcctcgcgct gtatcaagaa aaagggccgg aatttttcga cgatttgcag ggcatgttcg
     8821 cttttgccct gtacgacagc gaaaaagatg catacctgat tggccgcgac catctgggga
     8881 tcatcccact gtatatgggc tatgacgaac atggtcagct gtatgtggcc tcagaaatga
     8941 aagccctggt gccggtatgc cgcacgatta aagagttccc ggcggggagc tatttgtgga
     9001 gtcaggacag cgaaatccgt tcttattatc accgcgactg gttcgactac gatgcagtga
     9061 aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc
     9121 atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg tggtctggat tcctcaatta
     9181 tttccgcgat caccaagaaa tacgccgccc gtcgcgtgga agatcaggaa cgctctgaag
     9241 cctggtggcc gcaattacat tcctttgctg taggtctgcc gggttcaccg gatctgaaag
     9301 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
     9361 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
     9421 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
     9481 aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctc tacttccaca
     9541 aagcaccgaa tgccaaagaa ctgcatgaag agacggtgcg taaactgctg gccctgcata
     9601 tgtatgactg cgcgcgtgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc
     9661 cgttcctcga caaaaaattc cttgatgtgg cgatgcgtat taacccacag gataaaatgt
     9721 gcggtaacgg caagatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg
     9781 caagtgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg
     9841 acaccctgaa agaagtagcg gcacagcagg tttctgatca acagctggaa actgcccgct
     9901 tccgcttccc gtacaacacg ccgacgtcca aagaagcgta tctgtaccgg gagatctttg
     9961 aagaactgtt cccgcttccg agcgccgctg agtgcgtgcc gggcggccct tccgtcgcgt
    10021 gttcttccgc taaagcgatc gagtgggatg aggcgttcaa gaaaatggac gatccgtctg
    10081 gtcgtgcggt tggcgttcac cagtcggcgt ataaataaga cgtataatat tgccccggag
    10141 aaatccgggg caatttcagt tactggggcg cttgttgatt ctgatatgca gtaaggaaat
    10201 gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt atttcattga
    10261 ttaatttccc accactattg ttgaattccg aatttcatcc tgtatgacaa aagaatggct
    10321 gtagggtggg gaaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc
    10381 gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca
    10441 aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaaagatgg
    10501 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    10561 ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca ccagatttag
    10621 gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag
    10681 cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc
    10741 tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc gcccggttat
    10801 tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc
    10861 caggtacccc agccatcgaa gaaacaatct ggctacgtag ctcagttggt tagagcacat
    10921 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg
    10981 tcgaatatgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag cggtaaggca
    11041 ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca atttattcaa
    11101 gacgctgacc ttgtaagtgc acccagttgg ggtatcgcca agcggtaagg caccggattc
    11161 tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt
    11221 cggcgagctt tttgcttttc tgcgtatatt caatgtcgaa tgcgatgttg acacgtctta
    11281 tccttcaaag tcggatgcga cgctgccgcg tcttatccga cctacggttg gcacgcatcc
    11341 ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacatgcc
    11401 agcacgctcc gccgccatca aaccaagatt acgcacaaaa cgcagtggtg gcagattatt
    11461 gctgaatccg gcatagaaca gatccatacc gctctgcata atgaagttat ccgccatgcg
    11521 ccgcatctgg taacgcttga ggatcggata actggcccac gcttcgccat agctgcgagc
    11581 gttgaccaga acgtcaatca gggcatcgac atcacgataa ccaagattca ccccctgccc
    11641 cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata
    11701 ctgcaacgca tggcggcgcg tcagcggaaa agcgccagcg gcaagcggtg ttacataccc
    11761 aagacgcgac gggaaatgct tcgcgatttc cgcctgtaac tgcgccatat tcatattctg
    11821 caactggcga atgcgtgccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa
    11881 cggcagaaac gcacgaggtc catccggggt aaattgctgc caggtgctgt cgccgggatc
    11941 gttctcacac tgtacgctaa ttaacataca cgactgtgca tactgccagg catgaacgcc
    12001 aattcccgcc atctgccgca cctgggaatt tgcgccgtcg gcaccaatca ccagcttcgc
    12061 gcggatcgtt tcaccgccct tcagttccag ctcctgaaga tcattatggc gatgcagcgc
    12121 aatcagcgag cctggcacac gtaacgttac tttcggatgc gcttccagcg cctgccacag
    12181 cgcctgttgc agaacagtgt tttccaccat gtagccaagc acaggcagct ttaattcggc
    12241 tgcatcaaac accacatgcg ccgtttccca ctcccaagtt tccagtctgc ggtaaggatg
    12301 gcaacgcata gcctgtaccg cgtcccagac gcctaaacct ttaagcaatg ataccgaagc
    12361 cgcactgatc gccgagatcc gcacgtccgg ctggctatca acaacaaacg tcgcagattg
    12421 ggcatgttcg ataaccgtta ccgaaaatcc gtgctgagcc agtcccagcg ccagtgcgcc
    12481 gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca
    12541 tcctgttagc aatatgcttt aagtttacag gatttttacc cctaagggct gataacgctc
    12601 atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct ggctactatt
    12661 tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa
    12721 cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac
    12781 cgacgtggcg gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc
    12841 tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga
    12901 cctgattatc ggcgtcggcg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg
    12961 cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat
    13021 caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa
    13081 gtttgaccgt ctgccggaac cgcgtgccga agggccgacc gcgtttgtct ccatcatgga
    13141 aggctgcaat aaatattgca cctactgcgt agtgccttac acccgtggtg aagaggtaag
    13201 ccgtccgtcc gacgatattc tgtttgagat cgcccagctt gcggctcagg gcgtgcgtga
    13261 agttaacctg ctcggccaga acgtgaacgc ctggcgcggt gagaactacg acggcaccac
    13321 cggatcgttt gccgatctgc tgcgtctggt tgcggcgatc gacgggatcg atcgtattcg
    13381 ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt accgcgacac
    13441 gccggagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc gcattctgaa
    13501 cctgatggga cgtacccata cagcgctgga gtacaaagcg atcatccgta agctgcgtgc
    13561 ggcgcgtccg gatattcaga tcagttctga cttcatcgtt ggcttccctg gcgaaaccac
    13621 cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag
    13681 ctttatcttc tccgctcgtc caggtacacc agccgccgat atggttgatg atgttccgga
    13741 agaagagaag aagcagcgtc tgtatattct gcaagagcgc attaatcagc aagcgatggc
    13801 atggagccgc cgaatgctcg gcaccaccca gcgtattctg gtagaaggta catcgcgtaa
    13861 gagcatcatg gagctttccg gtcgcactga aaataaccgc gtggttaact tcgaaggtac
    13921 accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct
    13981 gcgcggtaaa gtggtgcgta ctgaagatga aatgggtctg cgcgtggcag aaacaccgga
    14041 atcggtgatt gcccgtaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta
    14101 attctcaggc ccgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc
    14161 gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc
    14221 agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc
    14281 ctggagccag cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc
    14341 aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgataacca ctttaaactg
    14401 accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat
    14461 accgccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt
    14521 aaagaagcgc gggtactgga gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc
    14581 aatatcaaga ccaaacgcgg cgtaatcaag ccgcgcacac caaaccaggc gcagtacatt
    14641 gccaatattc tcgaccatga catcaccttc ggcgttggcc cggcgggtac gggtaaaacc
    14701 tacctggcag tggctgcggc agttgatgct ctggagcgtc aggaaattcg ccgtattctg
    14761 ctgactcgtc cggcggtaga agccggtgag aaactgggct tcctgcctgg cgatttaagc
    14821 cagaaagtag atccgtatct gcgcccgctg tacgacgcgc tgtttgaaat gctgggcttt
    14881 gagaaagtcg agaaactgat tgagcgcaac gttattgaag tcgcaccgct ggcctatatg
    14941 cgtggccgta cgctgaacga cgcgtttatc attcttgatg aaagccagaa caccaccatc
    15001 gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc
    15061 gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca cgccatcgaa
    15121 gtgctggccg atgttgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt
    15181 cacccggtgg tggcgcgtat cgttaacgct tatgaagcct gggaagaagc agaacaaaaa
    15241 cgtaaagcgg cgctggcagc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg
    15301 tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt
    15361 ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agagtcggaa gtgactattc
    15421 gcgtggtcga taccgccgaa agtcacagtc tgaatctgac ctatcgcggt aaggataagc
    15481 cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggaaatg tcgctactgg
    15541 gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac
    15601 tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc
    15661 acatcgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc
    15721 tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca
    15781 gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg agaacccata
    15841 cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt
    15901 tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct
    15961 ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct
    16021 cgaaggggtg atggacatcg cagaccaacg cgtccgcgac atcatgatcc cccgctccca
    16081 gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc
    16141 cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat
    16201 ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt
    16261 gttacgtcag gcagttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt
    16321 tcgctctcag cgttaccaca tggcgatcgt cattgacgaa ttcggtgggg tttccggtct
    16381 ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga
    16441 agaagatgat atcgactttc gtcagctgag tcgccatacc tggaccgtgc gcgcactggc
    16501 ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag aggttgacac
    16561 tatcggtggt ctggtgatgc aggcatttgg gcatcttccg gcgcgtggcg aaaccatcga
    16621 catcgacggt taccagttca aagtggcgat ggccgacagt cggcgtatta ttcaggttca
    16681 tgtcaaaatc ccggatgact caccccagcc gaagctggat gaataaaacc gaaactggat
    16741 agataactac atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc
    16801 gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgacgtct ggcctgcggc
    16861 gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgtcgtccac tccagtctgc
    16921 cgctattggc ttttgctggg gatttggtct ctttggcagc ggtattaact gggtctatgt
    16981 cagcatcgcg acctttggtg gaatgcctgg cccggttaac gtcttcctgg tggtgctgct
    17041 ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gtctgtggcc
    17101 gaaaaccacc tggctgcgcg tggcgattgc cgcccctgct ctctggcaag tgaccgagtt
    17161 tctgcgcggt tgggtgctga ctggcttccc gtggttacag ttcggctata gccagattga
    17221 tggcccgtta aaagggctgg caccgataat gggcgtggaa gccattaact tcctgctgat
    17281 gatggttagc ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc cgctggcggt
    17341 ggccgtcgtg ctgtttgccc ttcccttccc gctgcgttac atccaatggt ttaccccgca
    17401 accggaaaaa actattcagg tttcgatggt tcagggcgat attccgcaat cgctgaaatg
    17461 ggacggagac cagctactta atacgctgaa gatttactac aacgcaacgg caccgctgat
    17521 gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca
    17581 gcaaccgttc ctcaaagcac tggacggtga gttgcgcgat aaagggagct cgctggtaac
    17641 cgggattgtt gacgcgcgtc tcaataagca gaaccgctac gatacttaca acaccatcat
    17701 cacgctgggg aaaggtgcgc cgtacagcta cgaatcagcc gatcgctata acaaaaacca
    17761 tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcgccgtt
    17821 ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccac cgctgtcggt
    17881 aaacggtatt gagcttactg cggctatttg ctacgagatc attctcggcg agcaagtgcg
    17941 tgataacttc cgcccggata ccgattatct gctgactatc tccaacgatg catggtttgg
    18001 taagtctatt ggtccatggc agcacttcca gatggcgcga atgcgtgcgc tggagttggc
    18061 gcgcccgcta ctgcgtagca ccaacaacgg cattacggcg gtgattggtc cgcagggtga
    18121 gattcaggcg atgatcccgc agttcacccg cgaagtgttg accactaatg tgactccgac
    18181 caccggactc acaccgtacg cacgtaccgg caactggccg ctgtgggtac ttacggcgct
    18241 gtttggtttt gccgccgtgt tgatgagtct gcgtcaacga cgtaaataat cccttcttat
    18301 cgtgctggaa tacatcccgt atttcggcac gctcaccgga tgcggcatca gtgcgttatc
    18361 cggataacaa aatcccccta tccaactttg gcacatctat tgctttgtta tacaaggcaa
    18421 agcttgaacc agcatcagta caacccggtt gcaccacagc aatccacggg tagcaccaaa
    18481 agagtgcatt ttaagaattt tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa
    18541 taaaacagca atatctttac atttagccaa actaaatgtt acctaacagg cacaacactg
    18601 cacaataaag ttgcagacga taacaacaca aacactcaca atgggtatcc atgcgttctt
    18661 aacgcagaag ataaaggagt tggatatgca attacgtaaa cctgccacag caatcctcgc
    18721 cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggtag cgggcagtac
    18781 gctggacaaa atcgccaaaa acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc
    18841 tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt actccaacgc
    18901 cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa aactgattcc
    18961 gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg
    19021 ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca ctattttcgt
    19081 ggtcggtacg cgcctgctga ccaaaaaggg cggcgatatc aaagattttt ccgacctgaa
    19141 aggcaaagcc gtagtcgtca cttccggcac tacttctgaa gttttgctca acaaactgaa
    19201 tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg
    19261 caccctggaa agcggtcgtg ccgttgcctt tatgatggat gatgctctgc tggccggtga
    19321 acgtgcgaaa gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc agtctcagga
    19381 agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgacac
    19441 tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa
    19501 tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact
    19561 gttcaaagaa ccgaatgaca aagcactgaa ctaattacaa gaaccagggg cggaaattcc
    19621 agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg
    19681 ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac
    19741 cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac
    19801 aagccccgtt cggcaacacc acctatcttg gttggatctg gagtggtttt caggtcacga
    19861 tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt
    19921 tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc
    19981 gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg gagttgctgc
    20041 cagagaaaat cggcttgtgg tttaaggccg agctggatcc caatattcag tttttccttt
    20101 catccatgct ctgccttggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg
    20161 cgattcagtc gctgccgcgt gggcagaaga atgccgcgct ggcgatgggt ctgacactac
    20221 cgcaggctta ccgctacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga
    20281 cctcagagat gatgaacctg gtgaaaaact ccgccatcgc ctcgactatc ggtctggt
//
LOCUS       DARNVM010000082        20127 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.082, whole genome shotgun sequence.
ACCESSION   DARNVM010000082 DARNVM010000000
VERSION     DARNVM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.082"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..174)
                     /gene="cspA"
                     /locus_tag="SI621_002690"
     CDS             complement(<1..174)
                     /gene="cspA"
                     /locus_tag="SI621_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HEI4065387.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESG"
     gene            complement(455..745)
                     /locus_tag="SI621_002691"
     CDS             complement(455..745)
                     /locus_tag="SI621_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HEI4065388.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1179..1889
                     /locus_tag="SI621_002692"
     CDS             1179..1889
                     /locus_tag="SI621_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HEI4065389.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1939..2913)
                     /gene="ghrB"
                     /locus_tag="SI621_002693"
     CDS             complement(1939..2913)
                     /gene="ghrB"
                     /locus_tag="SI621_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HEI4065390.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3017..3676)
                     /locus_tag="SI621_002694"
     CDS             complement(3017..3676)
                     /locus_tag="SI621_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HEI4065391.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3829..6162
                     /gene="bisC"
                     /locus_tag="SI621_002695"
     CDS             3829..6162
                     /gene="bisC"
                     /locus_tag="SI621_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HEI4065392.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVLSSRGALTTGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPEKPQGIRGQDEFVRVSWDEALELIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVNAEKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELSPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYGYQDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6131..6571)
                     /locus_tag="SI621_002696"
     CDS             complement(6131..6571)
                     /locus_tag="SI621_002696"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4065393.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6568..7131)
                     /gene="tag"
                     /locus_tag="SI621_002697"
     CDS             complement(6568..7131)
                     /gene="tag"
                     /locus_tag="SI621_002697"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEI4065394.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7289..7987
                     /locus_tag="SI621_002698"
     CDS             7289..7987
                     /locus_tag="SI621_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4065395.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8216..9424
                     /locus_tag="SI621_002699"
     CDS             8216..9424
                     /locus_tag="SI621_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065396.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9748..11439
                     /gene="eptB"
                     /locus_tag="SI621_002700"
     CDS             9748..11439
                     /gene="eptB"
                     /locus_tag="SI621_002700"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HEI4065397.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11531..11607
                     /locus_tag="SI621_002701"
     tRNA            11531..11607
                     /locus_tag="SI621_002701"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11565..11567,aa:Pro,seq:cgg)
     gene            11642..11775
                     /locus_tag="SI621_002702"
     ncRNA           11642..11775
                     /ncRNA_class="other"
                     /locus_tag="SI621_002702"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11808..11943
                     /locus_tag="SI621_002703"
     ncRNA           11808..11943
                     /ncRNA_class="other"
                     /locus_tag="SI621_002703"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12572..14179
                     /gene="dppA"
                     /locus_tag="SI621_002704"
     CDS             12572..14179
                     /gene="dppA"
                     /locus_tag="SI621_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HEI4065398.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14330..15349
                     /gene="dppB"
                     /locus_tag="SI621_002705"
     CDS             14330..15349
                     /gene="dppB"
                     /locus_tag="SI621_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HEI4065399.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15359..16261
                     /gene="dppC"
                     /locus_tag="SI621_002706"
     CDS             15359..16261
                     /gene="dppC"
                     /locus_tag="SI621_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HEI4065400.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16272..17255
                     /gene="dppD"
                     /locus_tag="SI621_002707"
     CDS             16272..17255
                     /gene="dppD"
                     /locus_tag="SI621_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEI4065401.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLSDGRQSKCHYPLDDAGRPTL"
     gene            17252..18256
                     /gene="dppF"
                     /locus_tag="SI621_002708"
     CDS             17252..18256
                     /gene="dppF"
                     /locus_tag="SI621_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HEI4065402.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18286..19557)
                     /locus_tag="SI621_002709"
     CDS             complement(18286..19557)
                     /locus_tag="SI621_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEI4065403.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFTNITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20033..>20127
                     /locus_tag="SI621_002710"
     CDS             20033..>20127
                     /locus_tag="SI621_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEI4065404.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWL"
BASE COUNT         4802 a         5004 c         5344 g         4977 t
ORIGIN      
        1 gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt aaccatcgtt
       61 ctggatagca gagaagtgta cgaacacgtc tttagagcca tcgtcaggag tgatgaagcc
      121 gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg acatagtgta
      181 ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag attttgaagc
      241 gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga cttgccttac
      301 tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca cgcattggcg
      361 ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg gtcctgtcat
      421 caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc ttacttaatg
      481 ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc ttcacgcgtc
      541 tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg aaatcatcga
      601 tttttaatcc tgtccctttt cgtaactgct caatttcggt acaggatgtt gttctgtgcg
      661 tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg ctcaacagct
      721 catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg ggatcgtgaa
      781 ttgcatcgaa gctcattaag aatagtcggg aaaagcagac acagcggggt gaagtcaggg
      841 catgctctgg ctatgattcg attcagccag tcatttagcg tgacttatag ccacctaacg
      901 atctgataaa tgccgatata tctcttacca ctcccggttt gtaaagattt cgaaaatcta
      961 acgttctgcg aaggcgtaaa tctcggtcta caaaaaaagc aacggcacta tcatttatta
     1021 acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca ggaagattac
     1081 gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt
     1141 gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct
     1201 ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt gggtgttttg
     1261 ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca gtgatgcgta
     1321 gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt ccttttgtct
     1381 ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg gattccggtc
     1441 tgcgcccggt cgtagacagc gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa
     1501 gcggcccact gcgtgaaatg aacggttctc tgggcgtact ggcgcaacag ctgcaaaatg
     1561 cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagcgatgac ctgaaaccgg
     1621 tctttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg caaccgttga
     1681 ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac attgcccaac
     1741 aaggtactca ggtgagcttt gtcgcaaatg gcatccagtt cccgacgtca cagcaggcaa
     1801 gtgaatataa caaactgatt gcgccattac cggcacagca tcaggcgttt aatcaggcct
     1861 ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt atcgggcttt
     1921 actccacgca gtcgcggctt agtccgcgac gtgcggattc acacagttct tctcaacctt
     1981 tccttgtaac gcatcaatca aattatccac ggcacaggcg gccatgccat aacgcgtctc
     2041 atgggtggca gatccaatat gcggtactgc gacgacattt gccattgaga gcaacggcga
     2101 atctacggaa agtggctctt gttcgaagac atccagcccg gcggcgtgaa tttcgccttt
     2161 ctgcaatgca gcaatcagtg cattttcatc aaccaccggg ccacgtccgg cattaatgaa
     2221 aatggcggag gatttcattt tgccgaattg ttctgcgcca aatagatgat acgtctcatc
     2281 agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagtgtat ccaaatcgca
     2341 gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt tatagaggat
     2401 gggcatgttg aaaccaaagt gcgcacgttg tgccagcgcc ataccgatcc gtcccatccc
     2461 gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc
     2521 ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag cggtagacaa
     2581 caccagcgcc atcagcgtat cggcgacggt ttctgttaat acggttggcg tgtgcatcag
     2641 cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga cagagatcgt
     2701 tgatgtggca cgcagtttcg gcattttttc cagcaatgcg gcatcaacat tctcgtttga
     2761 acccagtaaa ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct
     2821 gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg
     2881 taaggctttg tagaggataa cggacggctt catgcttctc tccattcact gataaggcga
     2941 tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat ttctgcatca
     3001 cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg ttttgcgcct
     3061 taccttctgc agtgctgttg ctggcgattg ggttagccgg gccaaggccc tgagtacgga
     3121 tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc gcacgctgct
     3181 gggagagacg catgttcagg tcgtgaccac ccgtgctgtc ggtataacca atcacgttaa
     3241 ccgccgtttt cggatactct ttcagcacca ttgccacgcc ggtcagggtg ttagcgcctg
     3301 ccggtttcag ggtcgcgctg ctgctgtcga aggtcacatt gttcggcata ttgaggataa
     3361 tgttatcccc gctgcgggtt acgctaacac cagtgccacg cattttgtca cgcagcttcg
     3421 cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcaccc gctgctgcgc
     3481 caatcagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca atacccgcgc
     3541 caacgagaga gcccagacct gcgccgatag cagatttacc tgcttcgcgt tcgccggtgt
     3601 aagggttagt tgtgcagcca gataccgcca gagcgccact cactacggcg gcaataagat
     3661 aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca cgggttccgt
     3721 ggcgggagat tatgccgcgt gaacatgaag attattcctg ggaatactcg gaaatttgta
     3781 agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt ggccaactca
     3841 tcctcacgat attccgttct gactgccgcc cactgggggc ccatgctggt tgaaactgac
     3901 ggcgaaaccg tgcttagctc gcgtggcgcg ttaaccacag gaatggaaaa ctccttgcag
     3961 agcgcggttc gcgaccaggt tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa
     4021 ggctttctcg cgtcaccgga aaaaccgcaa ggcattcgcg ggcaggatga atttgttcgt
     4081 gtgagttggg atgaggcgct ggagcttatt caccaacaac ataaacgcat tcgtgaggct
     4141 tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg cgtgctgcat
     4201 aaggcctcga cattattaca acgctatatg gcgctggcag gcggttatac cgggcatctg
     4261 ggggattatt cgaccggtgc agcacaggcg atcatgccgt atgtcgtggg gggcagcgaa
     4321 gtttatcaac agcaaaccag ttggcccctg gtgctggaac atagcgatgt cgtggtgctg
     4381 tggagtgcta atccactcaa tacgctgaaa attgcgtgga atgcatccga tgagcagggg
     4441 ctttcttact tttccgcact gcgtgacagc gggaaaaagc tgatctgcat tgatccaatg
     4501 cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt gggtggcacc gcacatgggc
     4561 accgatgttg cgctgatgct ggggatcgcc catacgctgg tggaaaatgg ttggcacgac
     4621 gaagcgtttc tggcgcgttg caccaccggt tatgccgtct tcgcctctta tttgctgggc
     4681 gagagtgacg gaatagccaa aaccgccgaa tgggcggcag ggatttgtgg agttaacgca
     4741 gagaaaatcc gcgagctggc ggctcttttc caccaaaaca cgaccatgct gatggctggc
     4801 tggggaatgc agcgtcaaca gtttggtgag caaaaacact ggatgatcgt cacgctggcg
     4861 gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta tcattttgcc
     4921 aatggtggta accccacgcg gcgttctgcg gtgctctctt ccatgcaggg cagcttgcct
     4981 ggcggcaccg atgcggtaga taaaatccct gttgcccgca ttgttgaagc actggaaaac
     5041 cctggtggcg catatcaaca caacggtatg aaccgacatt tcccggatat tcgttttatc
     5101 tggtgggcgg gcggtgccaa ctttactcat catcaggata ccaatcgcct gatccgtgcc
     5161 tggcaaaaac cggagctggt ggtgatctct gaatgcttct ggacggctgc ggcgaaacac
     5221 gcggatatcg ttctgcctgc gaccacttcg tttgagcgta atgatctcac catgaccggc
     5281 gattacagca atcagcatct ggtgccgatg aaacaagtgg tgccgccacg ttatgaagcg
     5341 cgtaatgatt ttgatgtctt tgccgagttg agtgaacgtt gggagaaggg cggctatgcg
     5401 cggtttacgg aaggaaaaag tgagctgcaa tggctggaaa cgttttataa cgttgcccgg
     5461 cagcgcgggg caagccagca ggttgaattg tcgccatttg ctgagttctg gcaagccaac
     5521 cagttaattg agatgccgga aaacccggac agtgagcggt ttattcgctt cgccgatttt
     5581 cgccgcgatc cgcaggcgca tccattaaaa accgccagcg gtaagattga aatattctcg
     5641 cagcgtattg ccgattatgg ttaccaggat tgccctgggc atccgatgtg gctggagccg
     5701 gatgagtggc agggcaatgc cgaaccagaa cagttgcagg tactttctgc tcatccggca
     5761 catcgtctgc acagccagct gaattacagt tctctgcgcg aattgtacgc ggtggcaaat
     5821 cgtgagcctg tcaccattca tcctgacgat gcccaggcgc gcggcataca agatggcgat
     5881 attgttcggt tgtggaacgc acgcgggcaa attcttgccg gagcggtcat tagcgaggga
     5941 attaaacctg gcgtgatttg tattcatgaa ggggcatggc cggatctgga tttaaccgct
     6001 gacggtattt gtaaaaacgg cgcggtgaac gttctgacca aagatctccc cagctcgcgg
     6061 ctggggaatg gctgtgcggg taatacggca ctggcatggc tggaaaaata caacggtccg
     6121 gaactgacac ttacagcgtt tgaaccaccg gccagctcat aatccaggtg ggtagttggg
     6181 tttcatcctg ccatgcgcag tcgacaatgt gaaaaccctg cgcatggtaa aaatctatcg
     6241 ccggttgatt tttttgataa acctccagca tcagatgggg atggcgctgc tgcacatact
     6301 gcattagcgc cttaccaata ccgcgtctga cggccttcgg tgcgacaaac attgcagcca
     6361 gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct tcccagaccc
     6421 agttttgcgc attggcaaga taggcatccc gcaccagcgg aatgcaatca cgccagtaat
     6481 tcgcttttat aaagggatgc ccccaggttg tactttccag ccacagttcg aggatcgcgg
     6541 ggagttctga acgttgcgct tcccgaatca tggtttattt cccggatagc aacagcagcc
     6601 aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga ttgtggtacc
     6661 aacaaactta aaaccacgct ttttcagggc tttagataga gcgtcggagg cgggcgtaga
     6721 tgtagggatt tcgctcaacg ttgtggcttg tgtcacctgt ggttgatgat tgacaaacga
     6781 ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt
     6841 accaataatt gcctgaattt tcccgcgatg gcggataatc ccggcgtcct gtaccagtct
     6901 ttcgacatcc tcttcctgca ttgcagccac cttcaccgga tcgaactgat gaaagctggc
     6961 gcgatagttt tcgcgttttt tgaggacggt gatccacgat aatccagcct gctgcccttc
     7021 aaggcagatc atttcgaaca gttttttact gtcagtttca ggcacgcccc actcattatc
     7081 atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca tactttccct
     7141 cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc
     7201 aatatttaat acagggctac tatgcccgat atccttcata caatgacaat aatattgccg
     7261 aattcggctc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca
     7321 gctaagcctg ctggcgagtg tggtaatcag tgcctttttt ctcaacacag cttacgcctg
     7381 gcaacaagaa tatatcgttg atacgcaacc cggacattcc acagagcgtt acacctggga
     7441 tagtgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag
     7501 ggcgctggga ctggaagtca atctggcgga agaaactcct gtggatgtga ccagcagtat
     7561 gagtatgggc tggaattttc ctttatatga gcaggttaca accggcccgg tcgcggcatt
     7621 acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta ctaccacgct
     7681 ggccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg actcccggct
     7741 tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg agaatatctg
     7801 gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca actggctgga
     7861 tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gctgcctatg ctgcgctatc
     7921 tcaggcagaa aataccacta acaatagcga ctatctgtat acgatggggg ttagtgccag
     7981 attttaacgt tatagataca attgaaacca ttggataaca acagttgtgg cgatagtggg
     8041 tgctaactta ctaaataata aatttggtga ataattgtcg agtcattcat tcctgaacta
     8101 atgcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca taaagtgcac
     8161 ttcgttatgg ttgtcagcag agatttttcc tttttattac tgcaggaata ctgccatgac
     8221 accttcaaat tatcagcgta cccgctggtt gacactcatc ggtactatca ttacccagtt
     8281 tgcgctgggg tcggtttata cctggagcct gtttaacggc gcgctttctg ccaagctgga
     8341 tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc tggcaatttc
     8401 atcttctgtt gcggggaaat tacaggaacg ttttggcgtt aaacgcgtca ctatggcttc
     8461 cggcattttg ctgggattag gcttcttcct gacagcgcat tctgacaacc tgatgatgct
     8521 gtggttaagc gccggtgtgc tggtcggtct ggcggatggc gcgggttacc tgctgacgct
     8581 ctctaactgc gtgaagtggt tcccggagcg taaaggtctg atctccgcgt tcgctatcgg
     8641 ttcttatggt ctgggtagcc tggggttcaa atttatcgac acgcagctgc tcgaaacggt
     8701 gggtctggaa aaaacatttg tgatttgggg agcgattgcg ctggtgatga ttgttttcgg
     8761 cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg gtgtcgtgga
     8821 gaaagattac acgctggcag agtcgatgcg taaaccgcag tactggatgt tagcggtaat
     8881 gttcctgact gcctgcatga gcggcctgta cgtgattggg gtagcgaaag atatcgccca
     8941 aagtctggca caccttgatg tggtttccgc agccaatgca gtcactgtta tttccatcgc
     9001 caacctttca ggtcgtctgg ttctggggat actgtctgac aaaatcgccc gaatccgtgt
     9061 tatcaccatt ggtcaggtga tttcgctggt gggtatggcg gccctgctgt ttgcaccatt
     9121 gaatgcagtg acgttctttg cagcgattgc ctgcgtggcg tttaactttg gcggcactat
     9181 cacggtgttc ccgtcactgg tcagtgagtt cttcggcctc aataacctgg cgaaaaacta
     9241 cggtgtgatt tatctcggtt tcggtatcgg cagcatttgt gggtcgatta tcgcctcact
     9301 gtttggcggc ttctatgtga ccttctacgt gattttcgcc ctgctgattc tgtcactggc
     9361 gctctctacg acgattcgtc agccagagca gaaaatgttg cgtgaggcgc atggctccct
     9421 ttaatcatct gccgcgagct actgggcttt agccttagtg tgaatcaaac gcttcaacgc
     9481 aatgtataaa caatgacgcg taaaatttgg gccagttaac tggccctttt tactgctttt
     9541 tgtaacgcta aagtctcttt tcaaacttgc atttttgtaa atttgtgctt cgtgcacact
     9601 ctttccccac actttttccc tttgctgtgg tctacttatt cgcgcgtgta gattttactt
     9661 atctgactac ctccgcactt ttcccctgcc gggcctgaaa agccgttaag cagggtgtta
     9721 tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac acagcagaag
     9781 ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt tttttaccgc
     9841 cgcttcggca gctatgcgca cgattttacc gtctggaaag gcatttctgc tgttgttgaa
     9901 ctggccgcca ccgtactggt gaccttcttt ttactgcgtc ttctttcgct gtttggccgc
     9961 cgcagctggc gtattctggc atcgctggtg gtgctctttt ccgcaggtgc cagctattac
    10021 atgaccttcc ttaatgtagt cattggttat ggcatcatcg catccgtcat gaccaccgat
    10081 atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat cgccgttagt
    10141 gcattgcctc ttatccttat ctggaataac cgctgtcgct acaccttgct ccgacaactg
    10201 cgaaccccag ggcagcgtat tcgcagcctg gcggtcgtcg tactggcggg tattatggtt
    10261 tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc gaccggcgtt
    10321 gatttgccga gttatggcgg tgtcgtagcg aactcttatc tgccatcaaa ctggctttct
    10381 gcgttggggc tgtatgcctg ggcgcgggtc gatgaatctt ccgataacaa ttcattgctt
    10441 aatccggcga agaaattcac ttatcaggca ccgcaaaacg ttgatgacac ttatgtcgtg
    10501 tttatcatcg gtgaaaccac gcgttgggac catatgggca ttttcggcta tgagcgtaat
    10561 accacgccga agctggccca ggagaaaaat ctggcggcgt tccgtggcta ttcttgtgac
    10621 acggcgacca aactttctct gcgttgcatg tttgtacgag agggtggagc ggaagataat
    10681 ccgcagcgca cattaaaaga acagaacatt ttcgcggtgc tgaagcagtt aggattcagt
    10741 tctgacctct acgctatgca gagcgaaatg tggttctaca gcaacacgat ggcagacaac
    10801 attgcttatc gtgagcagat tggtgcggag ccacgtaacc gtggcaagcc ggtagatgat
    10861 atgttgctgg tagacgaaat gcagcaatcg ctggggcgca acccggatgg caagcatctg
    10921 atcattttgc ataccaaagg ttcgcacttt aactacaccc agcgttaccc gcgcagcttt
    10981 gcgcagtgga agccggaatg tattggtgtt gatagcggct gtaccaaagc gcagatgatc
    11041 aactcctatg acaactcggt gacctatgtg gatcacttta tctccagcgt gattgatcag
    11101 gttcgcgata agaaagcgat tgtgttctac gcagctgacc acggtgagtc aattaatgaa
    11161 cgcgagcacc tgcacggcac accgcgtgaa ctggcaccgc cggagcagtt ccgtgtgccg
    11221 atgatggtct ggatgtcaga taaatatctg gaaaatccgg tcaatgcaca ggcatttgcg
    11281 cagctgaaaa aagaagccga catgaaagtg ccacgccgtc atgtagaact gtacgacacc
    11341 atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa caacaactgg
    11401 tgccacatcc cgcaggcaag agaggcggcg gctaactaag aggcctgctg actttctcgc
    11461 cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc agtaagatgc
    11521 gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac gaaggggtcg
    11581 gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt
    11641 ttgcatctgc agcccatgag gatgagaacc tccgggggca gggaggttcg actcgagcga
    11701 agcgagagaa cgttgcgcca gcaacggccc gcagggcgag ccacgaagtg gcgagtaatc
    11761 ctctatcacc gaccaaattc gaaaagcctg ctcaacgagc aggctttttt gcatttgtag
    11821 cctatgagga tgagaacctc cgggggcagg gaggttcgac tcgagcgaag cgagagaacg
    11881 ttgcgccagc aacggcccgc agggcgagcc acgaagtggc gagtaatcct ctggcaccga
    11941 ccaaattcgg caccgcactc attgtgggat acagcgtatc cgtcttatcc gatctaaacg
    12001 gcaacactcc acacatttcc ctgccaaatt tgcgccccat atctcactat ccattaacgg
    12061 atttgtgaca gaaaccattg tcttttttta tatttgctta aatctttttt tgcgttactt
    12121 atggctggcg ctggcatttt ccgctgtgcg gtttagcgtt catggtatta atcgctcaga
    12181 tatccacctt ttcatcagat ttttttcaca aaatgcataa aacttaatca ccaattgttg
    12241 ctaattatta agctgattgt tctggttatg ttggagtagc acaaatttct ctgctgcaaa
    12301 tagctgtttg aagttttttt aatctttgtt tgtggattct caccgttggt gtaaatcccg
    12361 gtgggctgta ttgacgtttt cacattctgt tgacagattg taggtcacga ggggcatttt
    12421 atggaggatc cgcactgtaa cactgatgtt aattagtacg gcatccccac ctcataacgt
    12481 tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga caacccactg caaagggtta
    12541 aaacaacaaa catcacaatt ggagcagaat aatgcgtatt tccttgaaaa agtcagggat
    12601 gctgaagctt ggtctcagcc tggtggctat gaccgtcgca gcaagtgttc aggctaaaac
    12661 tctggtttat tgctcagaag gatctccgga agggtttaac ccgcagttgt ttacctccgg
    12721 tactacctat gacgcctctt ccgtgccgct ttataaccgc ctggttgaat ttaaaatcgg
    12781 caccaccgaa gtgattccgg gcctcgctga aaagtgggaa gtcagcgaag acggaaaaac
    12841 ctataccttc catctgcgta aaggtgtgaa gtggcacgac aacaaagaat tcaaaccgac
    12901 gcgcgaactg aacgccgatg acgtggtgtt ctcgttcgat cgtcagaaaa acgcgcaaaa
    12961 cccgtaccat aaagtctctg gcggcagcta cgaatacttc gaaggcatgg gcttgccgga
    13021 gctgatcagc gaagtgaaaa aggtggacga caacaccgtt cagtttgtgc tgactcgacc
    13081 ggaagcgccg ttcctcgctg acctggcaat ggacttcgcc tctattctgt caaaagaata
    13141 tgctgacgcg atgatgaaag ccggtacacc ggaaaaactg gacctcaacc caatcggtac
    13201 cggtccgttc cagttacagc agtatcaaaa agattcccgt atccgctaca aagcgtttga
    13261 tggctactgg ggcaccaaac cgcagatcga tacgctggtt ttctctatta cccctgatgc
    13321 ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag gtgatgccgt acccgaaccc
    13381 ggcagatatc gctcgcatga agcaggataa atccatcaac ctgatggaaa tgccggggct
    13441 gaacgtcggc tatctttcat ataacgtgca gaaaaaacca ctcgatgatg tgaaagttcg
    13501 ccaggctctg acctacgcgg tgaacaaaga cgcgatcatc aaagcggttt atcagggcgc
    13561 gggcgtggca gcgaaaaacc tgatcccgcc aaccatgtgg ggctataacg acgacgttca
    13621 ggactacacc tacgatcccg aaaaagcgaa agccttgctg aaagaagcgg gcctggaaaa
    13681 aggcttctcc atcgacctgt gggcaatgcc ggtacaacgt ccgtataacc cgaacgctcg
    13741 ccgcatggcg gagatgattc aggcagactg ggcgaaagtc ggcgtgcagg ccaaaattgt
    13801 cacctacgaa tggggtgagt acctcaagcg tgcgaaagat ggcgagcacc agacggtaat
    13861 gatgggctgg actggcgata atggggatcc ggataacttc ttcgccaccc tgttcagctg
    13921 cgccgcctct gaacaaggct ccaactactc aaaatggtgc tacaaaccgt ttgaagatct
    13981 gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc gttgaactgt acaagcaagc
    14041 gcaggtggtg atgcacgatc aggctccggc actgatcatc gctcactcca ccgtgtttga
    14101 accggtacgc aaagaagtta aaggctatgt ggttgatcca ttaggcaaac atcacttcga
    14161 aaacgtctct atcgaataat taaaagccat acccggtggc gctgtgcctc tgcgtgcagc
    14221 gccatccggc agcaatactt cattccctgc ccgacctggg cgggaattga tttgtgagca
    14281 atacagacac gcagttccag gctgcgggtc actacagaga atccgggtta tgttgcagtt
    14341 tattctccga cgtttgggac tcgtcatccc cacgtttatc ggtattaccc ttctcacatt
    14401 tgcctttgtc cacatgatcc cgggcgatcc ggtgatgatc atggcgggcg aacgtgggat
    14461 ctccccagag cgtcacgcgc agctgctggc tgaactcggc ttagataaac cgatgtggca
    14521 gcagtatctc cattacattt ggggcgtaat gcacggcgat ttaggcattt cgatgaaaag
    14581 ccgcatcccg gtatgggaag agttcgtgcc gcgctttcag gccacgctgg aacttggcgt
    14641 ctgcgcgatg atttttgcta cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa
    14701 acgcggttcc attttcgatc acacggcggt tggcctggcg ctgacaggtt attcaatgcc
    14761 tatcttctgg tggggcatga tgctgatcat gctggtttcg gtgcactgga acctgacgcc
    14821 cgtctccggt cgcgtgagcg acatggtgtt cctcgatgat tccaatccgt taaccggttt
    14881 tatgctaatc gacaccgcca tctggggtga agacggtaac tttatcgatg ccgtcgccca
    14941 tatgatcctg cccgccattg tgctgggtac tatcccgctg gcggtcattg tacgtatgac
    15001 acgctcctcg atgctggaag tgctgggtga agattatatc cgcaccgcgc gcgccaaagg
    15061 gttgacccgc atgcgggtga ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtagt
    15121 gaccgttatc ggcctccagg tgggaacatt gctggcgggg gcgattctga ccgaaaccat
    15181 cttctcgtgg cccggtctgg ggcgctggtt gattgacgca ctgcaacgcc gcgactatcc
    15241 ggtagtgcag ggcggcgtat tgctggtggc gacgatgatt atcctcgtca acttgctggt
    15301 cgatctgctg tacggcgtgg tgaacccgcg tattcgtcat aagaagtaag gggacatcat
    15361 gtcacaggtt actgaaaata aagtgattag cgcaccggtg ccgatgaccc cgttacagga
    15421 gttctggcac tattttaaac gcaacaaagg cgcggtcgtc gggctggttt acgtcgtcat
    15481 cgtgctgttt atcgcgatct ttgccaactg gattgcaccc tataacccgg cggaacagtt
    15541 ccgcgatgca ctgctcgccc cgccagcctg gcaggaaggc ggcagcatgg cgcacttgct
    15601 gggcaccgat gacgtaggcc gtgatgtgct gtcgcgtctg atgtacggtg cgcgattgtc
    15661 actgctggtt ggctgtctgg tagttgtgtt atcgctgatt atgggcgtta ttctcggcct
    15721 gatcgccggt tacttcggag gcctggtcga taacatcatc atgcgcgtgg tcgatatcat
    15781 gctggcgctg ccaagtttgc tgttggcact ggtgctggtg gcaattttcg gcccgtcgat
    15841 tggtaacgcc gcgctggcat tgaccttcgt tgccttgccg cactatgtgc gcttaacccg
    15901 cgccgccgtg ctggtggagg tgaatcgcga ttacgttacc gcgtctcgcg tggcaggtgc
    15961 cggggcgatg cgtcagatgt ttattaacat cttcccgaac tgccttgcgc ctctgattgt
    16021 tcaggcgtcg ctcggtttct ctaacgccat tctcgatatg gctgctcttg gtttcctcgg
    16081 catgggggcg cagccgccaa cgccggagtg gggcaccatg ctctccgacg tgttgcagtt
    16141 cgcgcaaagc gcctggtggg tcgtgacctt cccgggtctg gcgatcctgc tgacggtgct
    16201 ggcatttaac ctgatgggtg acggtctgcg tgacgcgctc gatcccaaac tgaagcagta
    16261 agaggttcga gatggcgtta ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa
    16321 gcgcaccgtt ccgcgccgta gaccgcatca gctacagcgt aaaacagggc gaagtggtcg
    16381 ggattgtggg tgagtccggc tctggtaaat cggtcagttc gctggcgatt atggggctga
    16441 ttgattatcc gggccgcgta atggcggaaa agctggagtt taacggtcag gatttacagc
    16501 gtatctcaga aaaagagcgc cgcaacctgg tgggtgccga agtggcgatg atcttccagg
    16561 acccgatgac cagccttaac ccgtgctaca ccgtgggttt ccagattatg gaagcgatta
    16621 aggtgcatca gggcggcaac aaaagtaccc gccgtcagcg ggcgattgac ctgctgaatc
    16681 aggtcggtat tcccgatccg gcatcgcgtc tggatgttta cccgcatcag ctttccggcg
    16741 gcatgagcca gcgcgtgatg atcgccatgg cgattgcctg tcgaccaaaa ctgctgattg
    16801 ccgatgagcc gaccactgcg ctggacgtga ccattcaggc gcaaatcatc gaactgttgc
    16861 tggagctaca gcagaaagag aacatggcgc tggtgttgat tacccatgac ctggcgctgg
    16921 tggcggaagc ggcacataaa atcatcgtga tgtatgcagg ccaggtggtg gaaacgggcg
    16981 atgcccacgc catcttccat gcgccgcgtc atccatatac tcaggcattg ctgcgtgcgc
    17041 tgccggaatt tgctcaggac aaagaacgtc tggcgtcgtt gccaggtgtt gttcccggca
    17101 agtatgaccg cccgaacggc tgcctgctta acccgcgctg cccctatgcc actgacagat
    17161 gtcgcgctga agaaccggcg ctgaatatgc tctctgacgg gcgtcagtcc aaatgccatt
    17221 acccacttga tgatgccggg aggccgacac tatgagtacg catgaggcca cctcgcaaca
    17281 accgctgttg caggctatcg acctgaaaaa acattatccg gtgaagaaag gtatgttcgc
    17341 gccggaacgt ctggtgaagg cgctggacgg cgtttcgttt aaccttgaac gtggcaaaac
    17401 gctggcagta gtaggtgaat ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat
    17461 gatcgaaatg cccaccggtg gtgagctgta ttaccagggg caggatctgc tcaagcacga
    17521 tccgcaggcg cagaagctgc gtcggcagaa aatccagatt gtcttccaga acccttacgg
    17581 ttcgctgaat ccacgtaaaa aagtcgggca aattcttgaa gagccgctgc ttattaatac
    17641 cagcttaagc aaagaacagc gtcgggaaaa agccctgtcg atgatggcga aagtcggcct
    17701 gaaaaccgaa cattatgacc gctatccgca tatgttctcc ggcggtcagc gtcagcgtat
    17761 cgccattgcc cgtggtctga tgctcgaccc ggatgtggtg attgccgatg aaccggtttc
    17821 cgcgctggac gtttcagtgc gtgcgcaggt gctgaatctg atgatggatt tgcagcagga
    17881 gttggggctg tcttatgtct ttatctcgca cgacctgtca gtggttgagc acattgccga
    17941 tgaagtgatg gtgatgtacc tgggccgctg cgtggagaag ggaacgaagg accaaatctt
    18001 caataacccg cgtcatccgt acactcaggc gctactctcc gcgacgccgc gcctgaaccc
    18061 ggacgatcgc cgcgagcgca tcaagctcac cggtgaactg ccaagcccgc tcaatccacc
    18121 gccgggctgc gcgtttaacg cccgctgtcg tcggcgcttc ggcccctgca ctcagttgca
    18181 gccgcagcta aaagactacg gcggtcaact ggtagcttgt tttgctgttg atcaggatga
    18241 aaatccgcag cgttaactca caaaaccggg gccatgcgct ccggtttact caataagctt
    18301 caacagtggc gacaagcata acaaaatgcc gtacagcaga atcagccagg ctttaaaccc
    18361 gcgaagtttt tccagttgtg cgactttata gatcaggaaa aatggaatga ggcacgacac
    18421 aatgccatat aacgggcttc ccaactgaaa gaacaccagc accgatacac gaaacgaaac
    18481 ccaaatcgtc aacgtaatga cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt
    18541 aattttcttg gtatcaataa ttcggcttaa cagattgaga ataatgcctt taatagcctc
    18601 gtggaaaccg agataaatgc caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt
    18661 agaggtgata tgaatgatat gcccagggat cacctgcgcg gccagtgcca gtgccgagat
    18721 attttgttca aaggcagaaa cggcttcttc gtggctaatt gagaaagtaa acgaaaaggc
    18781 aaaaaacagg atcaccgcga tgagcgtgat ataactaatt cggtgggtac gcagcgcgag
    18841 ccgggttgcc agtactttat ctgcttcccg tttacgatag gcaatattca ttgggttaag
    18901 tacctgaata aatactgcag aaaagaagca aaatggaatg gtaagcagga catcgcggaa
    18961 aaagacggag gcttgcggga aggcggttat attggtgaaa ttccagtgcg ggatcatcgc
    19021 aaaaccgaac acgacgataa tccctacttt gaccaccacc attggcccgg aaatcttaaa
    19081 cagtaatcgt tcaccaccag acgcaattgc caccagtacg gcgaaaatgg cgactttata
    19141 aagtagagat tgtgaaagat cggcatcggt taaaccgaag gttttcaggt acgaggcgct
    19201 gtcgaaaacc acggagagag agtagataaa aatcccgtgg ataatcatca aaaagtagat
    19261 aaccccgagg aaaattcccc agttcttccc caggtaatga ctgataatat cggtgtagtc
    19321 attacaggaa tcgctttcag aaagggtttt taaataaatg tcctgcacta cccaggtggc
    19381 aggataagca atgatcgctg cggtaataaa tacccaaatt cccttcaagc caatttgtac
    19441 tggcatcagc acggttccgg caccaatcgc catgcctatg cataataaaa cccagccgaa
    19501 gtcgtagcgt gtaaacggca atttctgatc gtgtttcggt agtgtgttgt gctgcataaa
    19561 gtcctgtcca gaaagaagtc ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa
    19621 caggtggaga agtcgaatac gcagtatgta aaaaatgagg caacagattc actggggagg
    19681 tcgcgaggag gcgcaattct acaaaattgc gcatttcact accagtctgg agtcagctcg
    19741 gatatgaact gcaactattg caaataaaga aattaagctg gaaggcatcg cgcaatgagg
    19801 aagattgccg ggtgctgacg gtaaaaagca atcgtaaaat cagcggcaag ccggaaaggt
    19861 tccggtgagg cgcaatgttg cgggggctta atccctggtg gcattggttg ctggagagag
    19921 aaaacccccg cacgttgcag gtatgcacct gacaacacca cgggggccat tcttgcatcc
    19981 aaacaataca agaatagccg cgaaacgttg tcattacaac tcaggcggct atatgacgct
    20041 cgcagagctg ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct
    20101 tgccagcatg atcgtgaact ggctgaa
//
LOCUS       DARNVM010000083        20112 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.083, whole genome shotgun sequence.
ACCESSION   DARNVM010000083 DARNVM010000000
VERSION     DARNVM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.083"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(148..960)
                     /locus_tag="SI621_002711"
     CDS             complement(148..960)
                     /locus_tag="SI621_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEI4065405.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1222..1983
                     /gene="udp"
                     /locus_tag="SI621_002712"
     CDS             1222..1983
                     /gene="udp"
                     /locus_tag="SI621_002712"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEI4065406.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2124..3551
                     /gene="rmuC"
                     /locus_tag="SI621_002713"
     CDS             2124..3551
                     /gene="rmuC"
                     /locus_tag="SI621_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEI4065407.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3646..4401
                     /gene="ubiE"
                     /locus_tag="SI621_002714"
     CDS             3646..4401
                     /gene="ubiE"
                     /locus_tag="SI621_002714"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEI4065408.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4415..5020
                     /gene="ubiJ"
                     /locus_tag="SI621_002715"
     CDS             4415..5020
                     /gene="ubiJ"
                     /locus_tag="SI621_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HEI4065409.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5017..6657
                     /gene="ubiB"
                     /locus_tag="SI621_002716"
     CDS             5017..6657
                     /gene="ubiB"
                     /locus_tag="SI621_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEI4065410.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFIGWRKTR"
     gene            6736..7005
                     /gene="tatA"
                     /locus_tag="SI621_002717"
     CDS             6736..7005
                     /gene="tatA"
                     /locus_tag="SI621_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEI4065411.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7009..7524
                     /gene="tatB"
                     /locus_tag="SI621_002718"
     CDS             7009..7524
                     /gene="tatB"
                     /locus_tag="SI621_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HEI4065412.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7527..8303
                     /gene="tatC"
                     /locus_tag="SI621_002719"
     CDS             7527..8303
                     /gene="tatC"
                     /locus_tag="SI621_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HEI4065413.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMT
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8345..9127
                     /gene="tatD"
                     /locus_tag="SI621_002720"
     CDS             8345..9127
                     /gene="tatD"
                     /locus_tag="SI621_002720"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HEI4065414.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARALDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLFPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAAITDANVKTLFGIAF"
     gene            complement(9124..9612)
                     /gene="rfaH"
                     /locus_tag="SI621_002721"
     CDS             complement(9124..9612)
                     /gene="rfaH"
                     /locus_tag="SI621_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HEI4065415.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKMVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9779..11272
                     /gene="ubiD"
                     /locus_tag="SI621_002722"
     CDS             9779..11272
                     /gene="ubiD"
                     /locus_tag="SI621_002722"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEI4065416.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIISGDDVDLHRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11318..12019
                     /gene="fre"
                     /locus_tag="SI621_002723"
     CDS             11318..12019
                     /gene="fre"
                     /locus_tag="SI621_002723"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HEI4065417.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12210..13373)
                     /gene="fadA"
                     /locus_tag="SI621_002724"
     CDS             complement(12210..13373)
                     /gene="fadA"
                     /locus_tag="SI621_002724"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HEI4065418.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPT
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     NREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(13383..15572)
                     /gene="fadB"
                     /locus_tag="SI621_002725"
     CDS             complement(13383..15572)
                     /gene="fadB"
                     /locus_tag="SI621_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HEI4065419.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAIMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKRAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15762..17093
                     /gene="pepQ"
                     /locus_tag="SI621_002726"
     CDS             15762..17093
                     /gene="pepQ"
                     /locus_tag="SI621_002726"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HEI4065420.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17093..17707
                     /locus_tag="SI621_002727"
     CDS             17093..17707
                     /locus_tag="SI621_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HEI4065421.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17746..19197
                     /gene="trkH"
                     /locus_tag="SI621_002728"
     CDS             17746..19197
                     /gene="trkH"
                     /locus_tag="SI621_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HEI4065422.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19209..19754
                     /gene="hemG"
                     /locus_tag="SI621_002729"
     CDS             19209..19754
                     /gene="hemG"
                     /locus_tag="SI621_002729"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HEI4065423.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKSTLK"
BASE COUNT         4683 a         5107 c         5485 g         4837 t
ORIGIN      
        1 cttatgacta ttacttgccg actttcttaa ggtcaaatag cggagtcgtg ctctccaatc
       61 aattcatact gattattttt tgggggacag agatggcagc aaaacgcact cgttcactca
      121 ccatctcaaa tatagactaa gaggaaacta attccgcttc ccaccatact gcttaaacca
      181 ctccagcata cgctgccagc catctttcgc agattcggca tgatagctcg ggcgataatc
      241 ggcgttgaat gcatgcccgg catccgggta gacgataatc tctgctttcg cattagcagc
      301 ccgcagcgcc tggcgcatgg tttcaacgct ctcctgcgga atgctgttat cctgaccacc
      361 atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt gtttcggtga
      421 attcagcgac ttatcgcctg tcagtttgcc gtaccatgcc accgccgctt tcagttgcgg
      481 gttatgcgca gcatacagcc aggtgatacg tccaccccag cagaatccgg tgatcattaa
      541 acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga gatcggccag
      601 cacctgcgag tcaggcactt ttgctaccag accgctaagt aacgtgggga tatcggcaaa
      661 atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat acccctccag
      721 cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt cctgcactac
      781 aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag caggcatgtt
      841 atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtcca gggtctgaac
      901 gatggtcgaa gctagaggcg atgcagcagg tgcaaatcca gattgttgtg ttgttgccat
      961 ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca gcatgcaaca
     1021 aatcacattg cctgaatcgg ctcatctttt atgcagtcct gcagaatgaa gagtgattta
     1081 tgtgatttgc atcacttttg gtgggtaaat ttatgcaacg catttgcgtc atggtgatga
     1141 gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt ctgactaaac
     1201 cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg cctcactaaa
     1261 aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg tgtggaaaag
     1321 atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt caccacctgg
     1381 cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg cggcccgtct
     1441 acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct gcgtatcggt
     1501 acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac cacggcgtct
     1561 gttcgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc tgtcgctgat
     1621 ttcgaatgta cgactgcgct ggttgaggct gcgaaatcca tcggcgcgac gactcacgtt
     1681 ggcgtgacgg cttcttctga taccttctac ccaggtcagg aacgttacga tacttactct
     1741 ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat gggcgtaatg
     1801 aactatgaaa tggaatctgc aaccctgctg accatgtgtg caagtcaggg cctacgtgcc
     1861 ggtatggtag cgggtgttat cgttaaccgc acccagcaag agatcccgaa tgctgagacg
     1921 atgaaacaaa ctgaaagcca agcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg
     1981 taatcctctt ctcctgtctg aaggccgacg cgttcggcct tttgtatttt tgcgtagcgc
     2041 ctcgcaggaa atgcctttcc aactggacgt ttgtacagca caattctatt ttgtgcgggt
     2101 aagttgttgc gtcaggaggc gttgtggatt tctcaatcat ggtttacgca gttattgcgt
     2161 tggtgggtgt ggcaattggc tggctgtttg ccagttatca acatgcgcag caaaaagccg
     2221 agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa caacaaatta
     2281 cccaaagcga gcactggcgc gctgagtgcg agttactcaa taacgaagtg cgcagcctgc
     2341 aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg atggaagccg
     2401 cacagcaaca tgctgacgat aaaattcgcc agatgattaa cagcgagcag cgcctcagtg
     2461 agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg gttgatgagc
     2521 aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg gacggtttcc
     2581 gccgtcaggt tcaggacagc tttggtaaag aagcacaaga acgccatacc ctgacccacg
     2641 aaattcgcaa tctccagcag ctcaacgcgc aaatggccca ggaagcgatc aacctgacgc
     2701 gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtagta ttgacgcggg
     2761 tgctggaggc ttccggtctg cgtgaagggt atgaatatga aacccaggtc agcatcgaaa
     2821 atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg gttgccgcag ggaaaagatg
     2881 tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctatttcaac gccgaagatg
     2941 actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac catatccgtt
     3001 tgctgggacg caaagattat caacagctgc cggggctgcg aaccctggat tacgtgctga
     3061 tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg gagctgatca
     3121 ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac cacgctgctg gtggcgctgc
     3181 gcactatcgc caacctctgg cgctatgagc atcaaagtcg taacgcccag aaaatagccg
     3241 atcgcgccag caagctgtac gacaagatgc gtttgttcgt cgatgacatg tcagcgattg
     3301 gtcaaagtct cgacaaagcg caggataact atcgccaggc aatgaaaaaa ctctcttcag
     3361 gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggggta gaaattaaac
     3421 gcgagattaa tccggatttg gctgaacaag cggtgagcca ggatgaagag tatcgactgc
     3481 ggtcggttcc ggagcagccg aatgatgaag cttatcaacg cgatgatgaa tataatcagc
     3541 agtcgcgcta gcccattggg ggtagttaag ccgggtagaa atctagggca tcgacgccca
     3601 atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt ggataagtca
     3661 caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc ggatatggtc
     3721 gcccacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt gatgtcattt
     3781 ggtattcatc gtttgtggaa gcgattcacg attgattgca gcggcgtacg ccgtgggcag
     3841 accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc ccgcctggtc
     3901 ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa aatgggccgc
     3961 gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc gaacgctgag
     4021 gcgctgccgt tcccggataa cacctttgat tgcatcacta tttcgtttgg tctgcgtaac
     4081 gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc cggcggccgc
     4141 ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc ctatgatgca
     4201 tactccttcc atgtgctgcc gcgtattggc tcattggtcg cgaacgacgc cgacagctac
     4261 cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa agccatgatg
     4321 caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt tgtggcgctg
     4381 catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt tagtgacggc
     4441 aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga aaacggcccg
     4501 ctcgcgtctg ttgggcaaag tgctgcgcgt ggaggtaaaa ggcttttcga catcattgat
     4561 tctggtgttc agcgaacgcc aggttgatgt actgggcgaa tgggcaggcg atgctgactg
     4621 caccgttatc gcctacgcca gcgtgttgcc gaaacttcgc gatcgccagc agcttgccgc
     4681 actgattcgc agtggtgagc tggaagtaca gggcgatatt caggtggtgc aaaacttcgt
     4741 tgcgctggca gatctggcag agttcgaccc tgcggaactg ctggcccctt ataccggtga
     4801 tatcgccgct gaaggtatca gcaaagccat gcgcggaggc gcaaagttcc tgcatcacgg
     4861 cattaagcgc cagcaacgtt atgtggcaga agccattact gaagagtggc gtatggcacc
     4921 tggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc gtgctgttga
     4981 tgccctgacc aaacggctgg aaaaactgga ggctaaatga cgccaggtga agtacggcgc
     5041 ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat ccccaaaatg
     5101 cgtatcaccc tgccactacg gctatggcga tactcattat tctggatgcc aaatcggcat
     5161 aaagacaaac ctttaggtga gcgactacga ctggccctgc aagaactggg gccggtatgg
     5221 atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt ttccgccgca tattgccgat
     5281 cagctggcgt tattgcagga caaagtcgct ccgtttgatg gcaagctggc gaagcagcag
     5341 attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt tgaaatcaag
     5401 ccgctggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc gaatggtaaa
     5461 gaggtggtga ttaaagtcat ccgcccggat attttgccgg ttattaaagc ggatctgaaa
     5521 cttatctacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg ccgtctgcgc
     5581 ccaaccgaag tggtgcgcga gtacgaaaag accttgattg atgaactgaa tttgctgcgg
     5641 gaatctgcca acgccattca gcttcggcgc aattttgaag acagcccgat gctctacatc
     5701 ccggaagttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg catttacggc
     5761 attccggtgt ctgatgttgc agcgctggag aaaaacggca ctaacatgaa attgctggcg
     5821 gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt ccatgccgat
     5881 atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa atatatcggc
     5941 attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct ggcagaaaac
     6001 tttatcgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt cgattctggc
     6061 tgggtgccac cagataccaa cgttgaagag ttcgaatttg ccattcgtac ggtctgtgaa
     6121 cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt aaatctgttt
     6181 aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact ccagaaaacc
     6241 ctgctctacg tcgaaggggt aggacgccag ctttatccgc aactcgattt atggaaaacg
     6301 gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc gctggtgaga
     6361 gcattcaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc tgaactggtt
     6421 tacgacagtt tgcgccaggg caagtattta cagcacagtg ttgataagat tgcccgcgag
     6481 cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat tggcgctacg
     6541 ttagtattaa gtggcacatt cttgttggtc agccgacctg aatgggggct gatgcccggc
     6601 tggttaatgg caggtggtct gatcgcctgg tttatcggtt ggcgcaaaac acgctgattt
     6661 tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc tttgtttaat catcatctac
     6721 cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat tgccgtcatc
     6781 gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct tggtgcgtcg
     6841 atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa aaccagtcag
     6901 gatgctgatt ttactgcgaa aactatcgcc gataagcagg cggatacgaa tcaggaacag
     6961 gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt gtttgatatc
     7021 ggttttagcg aactgctatt ggtgttcatc atcggcctcg tcgttctggg gccgcaacga
     7081 ctgcctgtgg cggtaaaaac ggtcgcgggc tggattcgcg cgttgcgttc actggcgaca
     7141 acggtgcaga acgaactgac ccaggagtta aaactccagg agtttcagga cagtctgaaa
     7201 aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc gatggatgaa
     7261 ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc tgaaaaggcg
     7321 agtgatgaag cgcacaccat ccacaacccg gtggtgaaag acaatgaagc tgcgcatgaa
     7381 ggcgtaacgc ctgctgctgc acaaacgcag gccagttcgc cggaacagaa gccagaaacc
     7441 acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc tgcaccttcc
     7501 ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg cttatcacgc
     7561 atctgattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc gtgatattcc
     7621 tgtgtctggt ctatttcgcc aatgacatct atcacctggt atccgcgcca ttgattaagc
     7681 agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc tttacgccga
     7741 tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc tatcaggtgt
     7801 gggcgtttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg ccgctgctgg
     7861 tttccagctc tctgttgttt tatatcggca tggcgttcgc ctactttgtg gtcttcccgc
     7921 tggcatttgg cttccttgcc aataccgcgc cggaaggggt acaggtatcc accgacatcg
     7981 ccagttattt aagcttcgtt atgacgttgt ttatggcgtt tggtgtctcc tttgaagtgc
     8041 ctgtggcaat agtgctgctg tgctggatgg ggattacctc gccagaagac ttacgcaaaa
     8101 aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gctgctgaca ccgccggatg
     8161 tcttctcgca aacgctgttg gcgatcccga tgtactgtct gtttgaaatc ggtgtcttct
     8221 tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac gctgaagcag
     8281 aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc atatggagta
     8341 caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag accgtgatga
     8401 tgttgtagca cgcgctcttg acgcgggagt taatgggcta ctcatcaccg gtactaacct
     8461 gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat tcatcctgtt ggtcaacggc
     8521 gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag cgattattga
     8581 gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact ttaaccgcaa
     8641 cttttcgacg ccggaagagc aggaacgcgc ttttgttgcc cagctacgca ttgccgcaga
     8701 attaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta tgacattgct
     8761 ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg gcacacgcga
     8821 agagatgcag gcgtgcgtgg cgcatggaat ttatatcggc attaccggtt gggtttgcga
     8881 tgaacgacgc ggactggagc tgcgggaact tttgccgttg attccggcgg aaaaattgct
     8941 gatcgagact gatgcgccgt atctgttccc tcgcgatctc acgccaaagc catcatcccg
     9001 gcgcaacgag ccagcccatc tgccccatat tttgcaacga attgcgcact ggcgtggaga
     9061 agatgccgca tggctggctg ccatcacgga tgccaatgtt aagacactgt ttgggattgc
     9121 gttttagagt ttgcggaact cggtgttctt cacactgtgc ttaatctctt tattgataag
     9181 attaagtaac agcatggagc gagcctcgcc gtcgggttcg gtgaaaatgg cctgaaaacc
     9241 ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggttg ccggatcgac
     9301 aatgtctttc ggtttatata ccgatagctg atgaatcacc gccgatggga ctatcgctgg
     9361 cgatgcgcca aagcgcacga agtggctgac accgcgggtc gcgttgatag tcgtggtatg
     9421 aatcacttct ggatcaaatt ccacaaacag gtagttgggg aacaatggtt cactgactgc
     9481 agtacgtttg ccacgcacca ttttttccag ggtgatcatc ggtgccaggc aattcacagc
     9541 ctgtctttcg aggtgttcct gggcacgttg aagttgcccg cgcttgcagt acagtaaata
     9601 ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc aaaagcttta
     9661 cgctaagtta attatattcc ccggtttgcg ttataccgtc agagttcacg ctaatttaac
     9721 aaatttacag catcgcaaag atgaacgccg tataatgggc gcagattaag aggctacaat
     9781 ggacgccatg aaatataacg atttacgcga cttcctgacg ctgcttgaac agcagggtga
     9841 gctaaaacgt atcacgctcc cggtggatcc gcatctggaa atcactgaaa ttgctgaccg
     9901 cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac cctaaaggct actcaatgcc
     9961 ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg atgggcatgg ggcaggaaga
    10021 tgtttctgcg ctgcgtgaag tcggtaaatt attagccttt ctcaaggaac ctgaaccgcc
    10081 aaaaggtttt cgcgacctgt ttgataaact gccgcagttc aagcaagtat tgaacatgcc
    10141 gacaaagcga ctgcgtggtg cgccgtgtca gcaaaaaatt atctccggcg atgacgtcga
    10201 tcttcaccgt atccccatta tgacctgctg gccggaagac gccgcgccgc tgattacctg
    10261 ggggttgacc gtgacgcgtg gcccgcataa agagcggcag aatctgggta tttatcgtca
    10321 acagttgatt ggtaaaaata aactgattat gcgctggttg tcgcatcgcg gcggcgcgct
    10381 ggatttccag gagtggtgtg cggcgcatcc gggcgaacgt ttcccggttt ctgtggcgct
    10441 gggtgccgat cccgccacga ttctcggtgc agtcaccccc gttccggata cgctttcaga
    10501 gtatgcgttt gccggattgc tgcgcggcac caaaaccgaa gtagtaaagt gtatttccaa
    10561 tgatctcgaa gtgcccgcca gtgcggagat tgtgctggaa gggtatatcg aacaaggcga
    10621 aactgcgccg gaagggccgt atggcgacca caccggttac tataacgaag tcgatagttt
    10681 cccggtattt actgtgacgc atattaccca gcgtgaagat gcgatttatc attccaccta
    10741 taccgggcgt ccgccggatg aacctgcggt gctgggtgtc gcactgaacg aagtgtttgt
    10801 gccgattctg caaaaacagt tcccggaaat tgtcgatttt tacctgccgc cggaaggctg
    10861 ctcttatcgc ctggcggtag tgacaatcaa aaaacagtac gccggacacg cgaagcgcgt
    10921 catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg tgatcgtttg
    10981 cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt tgggcgatta ctacccgtat
    11041 ggacccggcg cgggatactg ttctggtaga aaatacgcct attgattatc tggattttgc
    11101 ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat gccacgaata aatggccggg
    11161 ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg tcgcgcatat
    11221 tgacgccatc tgggatgaac tggctatttt taacaacggt aaaagcgcct gatgcgcgtt
    11281 tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa gctgtaaagt
    11341 gacctcggta gaagctatca cggataccgt atatcgtgtc cgcatcgtgc cagacgcggc
    11401 cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg acaaacgtcc
    11461 gttctcaatg gcctcgacgc cggatgaaaa agggtttatc gagctgcata ttggcgcttc
    11521 tgaaatcaac ctttacgcga aagcagtcat ggaccgcatt cttaaagatc atcaaatcgt
    11581 ggtcgatatt ccgcacggtg aggcatggct gcgcgatgat gaagaacgtc caatgattct
    11641 catcgcgggc ggcaccgggt tctcttatgc gcgctcgatt ttgctgacgg cgctggcgcg
    11701 taacccaaac cgtgatatca ccatttactg gggcgggcgt gaagagcagc atctttatga
    11761 tctctccgag cttgaggcgc tttcgcttaa gcatccaggt ttgcaagtgg tgccggtggt
    11821 tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc gtgttaacgg cggtattgca
    11881 ggatcatggt acgctggcag agcatgatat ctatattgcc ggacgttttg agatggcgaa
    11941 aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgcc tgtttggcga
    12001 tgcgtttgca tttatctgag atataaaaaa cccgcccctg acaggcggga agaacggcaa
    12061 ctaaaacttt atacagtggc atttagatct atgtcgcatc cggcattcgg cacacgatgc
    12121 ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg tagaccggat
    12181 aaggcgttca cgccgcattc ggcaagtggt taaatccgct caaacaccgt cgcaataccc
    12241 tgacccagac cgatacacat cgtcgctaga ccaaactggg catctttgcg ctccatcaga
    12301 ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc cagcgcgatc
    12361 gcgccaccgt tgaggttgat cttctcgtca atctgctcaa tcagtcccag atctttaata
    12421 catggcagga tctgcgcggc aaaggcttca ttcatttcaa acacgccgat atcactgaca
    12481 gaaagccccg cttttttcag tgccagcttc gaggccggaa ccgggccgta acccataatc
    12541 gatgggtcac aaccaacgac cgccatcgaa cgcacgcggg cgcgcggctt aagacctaat
    12601 tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga aagcgcagaa
    12661 gatgtacccg ccgttaccgt accgctgacg ggatcaaacg ccggacgcag cgtggcgagg
    12721 gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt caggacgccg
    12781 tcggcatcgt gaccaccggt cgggataatt tcatttttaa atgcacccga ctgcgtggca
    12841 gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg gttgataccg
    12901 tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt ggcgacattg
    12961 cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc catatgctcc
    13021 acgccgccaa ccagacatgc ctgcgcatcg ccggtcatga tcattcgtgc tgcatcatgc
    13081 agtgcctgca tggatgaacc acacaagcga ttgacggtaa ccgccgggac agagtgtggt
    13141 acttctgcca gcagcgccgc gttacgggcg atattaaaac cctgctccag cgtctgctgc
    13201 acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgtcgggtt acgcgccagc
    13261 aggctacgca ttaaatgagc ggagagatct tctgcacgca cgtgacgaaa agcaccgccc
    13321 ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc cattgtgact
    13381 ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggggg atagtacggt
    13441 tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg gccgaggtgc
    13501 tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag ccagcggaac
    13561 gcgccaccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc cgcttctgcc
    13621 ggagtggcga taatgccttc ttccagacag cgcaccactt cgttgaccat cgggatcatc
    13681 atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gctggctcac ttctgccagc
    13741 aggtcgtcaa cagcggcgtc ttcttctttc ttcggcttac ctttgctgtc ttctttataa
    13801 cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag cgcgtcgatg
    13861 gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac agcctgggca
    13921 tgatgcgcgg tgtcaatgcc cacaacgtcc agcagatatg ccggacccat aggccagcca
    13981 aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg cagcagctgg
    14041 ctgaaacccg cgaagtaggg gaacagcacg cggttaacga agaatccggg gcagtcgtta
    14101 accacaatcg gcgttttgcc catcttgctc gcccaggcga cgactttcgc gatggtttcg
    14161 tcggagcttt tctcgccgcg aataatttct accaacggca tccggtgtac cgggttaaaa
    14221 aagtgcattc cgcagaagtt ttccggacgt tccagcgcgt tggccagctc gctgatagga
    14281 atggttgaag tgttagacgc cagcacggta tccgggcgta ctttttgctc ggtttctgcc
    14341 agcacggctt ttttcacttt cgggttttcg acaaccgctt ctaccacaac atccacgcgg
    14401 tcaaatccgg cgtagtcgag cgttgggtgg attgtggaga tcacgccagc cagtttcaga
    14461 ccatcgatct tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc agtcatgccg
    14521 agggttaacg acttgtcgtt gatatctttc atgataaccg gcacaccttt ccacgcagac
    14581 tggtaagcga tgccgccgcc cataatgcct gcgcccagca cagcggcccg tttcggcgtt
    14641 tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt aaggaatatg
    14701 ccgaccaatg cgcgagcttc gttggtatgc gccagcggga caaaactttt gttttccagg
    14761 tttagggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac tgcggtgatg
    14821 ggggccggat aatgtttccc cgccgtttgt gcgaccatcc ctttagcgat ggtgaagctc
    14881 atgatggctt caatcttgct cagttttagc ggttccagct tcggctgacg tttcgctttc
    14941 cagtcgaggt cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc aatcagtttt
    15001 tctgctttga ctacaccatc caccagaccg attttcagcg cctgatccgc gccgacatct
    15061 ttaccggcgg caatgatttc cagcgcactg tcagcgccca gcatacgtgg catacgtaca
    15121 gaaccgccaa agcccggcat gatgcccagt ttggtttccg gcagaccgat gcgcagatcc
    15181 ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccgcc cagcgcatag
    15241 ccgttgacgg cagcaatggt cggcaccggg agatcttcca ggcgattaaa cacgctattg
    15301 gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga caaaaattcg
    15361 gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag cccttttaga
    15421 tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt cgcagtgtcg
    15481 agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc ttccagccag
    15541 tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg caaggtgatc
    15601 tggtcgtacc agatgcatcg aagtgtgtat tttgtgttaa aaatatgcaa ataaaagatt
    15661 aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa cgatgtgcta
    15721 agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca ctggcctcac
    15781 tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg gcgcgcttca
    15841 agctggatgc gttacttatt cactccggcg aactgttcaa cgtttttctc gacgatcatc
    15901 cctatccgtt taaagtgaat ccgcaattca aagcgtgggt gccggtaact caggtgccaa
    15961 actgctggct gttggtggat ggcgtgaaca agccgaaact gtggttttat ctgccggttg
    16021 attactggca caacgtcgaa ccgctgccga cctccttctg gactgaagat gtagaagtga
    16081 tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc gggaatatcg
    16141 gttatatcgg tccggtgccg gagcgtgcgc tgcaactggg tattgaggcc agcaatatca
    16201 acccgaaagg ggtgatcgac tacctgcatt actaccgctc cttcaaaacc gagtacgaac
    16261 tggcctgtat gcgtgaagcg cagaaaatgg cggtcaacgg tcatcgcgcg gcagaagaag
    16321 cgttccgttc tggcatgagc gagttcgata tcaatattgc ctatctgacc gcgaccggtc
    16381 atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac gctgcggtgt
    16441 tgcattacac caaactggac caccaggcac cggaagagat gcgcagcttc ctgctggatg
    16501 ccggggccga atataacggc tatgcggctg acctgacccg tacctggtcg gcaaaaagcg
    16561 ataacgacta cgcacagctg gtgaaagacg taaatgatga acaactggcg ctgatcgcca
    16621 ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag cgcatcgcca
    16681 aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg gtcgaaaacg
    16741 atctcaccgg accgtttatg ccgcacggta tcggtcatcc gctgggcctg caggtgcatg
    16801 acgtcgccgg ttttatgcag gatgatagcg gtacacacct cgcggcaccg gcaaaatatc
    16861 cgtacctgcg ctgcacccgt attctccagc cgggcatggt gttaaccatc gagccgggta
    16921 tctacttcat cgaatcgcta ctggcaccgt ggcgtgaagg gcagttcagt aaacacttta
    16981 actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa gacaacgtgg
    17041 tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg tgatggaaag
    17101 ctggttaatt cctgcggcac cagtcacggt cgttgaagag atcaaaaaga gccgcttcat
    17161 aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg aatcggtgcg
    17221 ggcagaacac cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg cgccggatga
    17281 ttctcaacag ctcggtttct ctgacgacgg ggaaccggcg ggaacggccg gtaaaccgat
    17341 gctcgcccaa ctaatgggca gcggcgtcgg ggaaattacc gctgtggtgg tgcgctacta
    17401 cggcggcata ttgcttggta ccggtgggtt agtgaaagcg tatggcggcg gagtgaatca
    17461 ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata ctttgcaatg
    17521 tgaatatagt cagttaaccg gcattgaagc gttgctgggg cagtgtgacg gcaaaattat
    17581 caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg cgaaagtggc
    17641 tgaattttcc gcaaaactgg cggattttag ccgtggttca ttgcaattgt tagcgattga
    17701 agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca ttttcgcgcc
    17761 attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat tatccctggg
    17821 ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac cttttttgtc
    17881 gccctcgcca ttggctctat gctgtggtgg ccgaaccgta aagagaaggg cgaactgaaa
    17941 tcccgtgagg ggtttctgat agtggtgctg ttctggaccg tgctgggtag tgtcggtgcg
    18001 ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt ttttgaatct
    18061 ttctctggcc tgaccaccac gggggccact acgctggtgg ggctggattc gctccctcac
    18121 gccatcctat tttatcgcca gatgctgcaa tggtttggcg ggatggggat catcgtgttg
    18181 gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg cgcagaaatg
    18241 cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc gaaaaccctg
    18301 tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt tgccggaatg
    18361 gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg cttctcgaca
    18421 catgatgcca gtatcggtta tttcgacagc ccgactatta acactatcat tgctatcttc
    18481 ctgctgatct ccggctgtaa ctacggtctg cacttttcgc tgttaagtgg gcgtagtctg
    18541 aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt tacgctggtg
    18601 gttatttgta ccctcgtact gtggtttcat aatgtctaca gttcggcgct gatgacaatt
    18661 aaccaggcgt ttttccaggt ggtgtcgatg gcgacaaccg ccgggtttac gactgacagc
    18721 attgctcgct ggccgctctt tttaccggta ctgcttttat gttcagcttt tatcggcggt
    18781 tgtgccgggt caacgggcgg tggcctgaaa gtgatccgca tcctgctgct gtttaagcag
    18841 gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat taagctgggg
    18901 aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc cgcctatgca
    18961 ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga cttttctgcc
    19021 tttgcctcag ttgttgcgac attgaataac ctggggccag ggcttggcgt ggttgctgat
    19081 aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat gctgtttggt
    19141 cgtctcgagg tctttacatt gctggtgctc tttaccccga ctttctggcg tgaatgatgg
    19201 agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg cgcgagattg
    19261 cctcctacct ggcttcggaa ctgaaagaac tggggatcca ggcggatgtc gccaatgtgc
    19321 accgcattga agaaccacaa tgggaaaact atgaccgtgt ggtcattggt gcttctattc
    19381 gctatggtca ctaccactct gcgttccagg agtttgtcaa aaagcatgcg acgcggctga
    19441 attcgatgcc gagcgccttt tactccgtaa acctggtggc gcgtaaaccg gagaagcgta
    19501 ctccacagac caacagctac gcgcggaagt ttctgatgag ttcacaatgg cgtcccgatc
    19561 gctgtgccgt cattgccggg gcgctgcgtt atccacgtta ccgctggtac gaccgtttta
    19621 tgatcaagct aattatgaag atgtcaggcg gtgaaacgga tacgcgtaaa gaagttgtct
    19681 ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta accgacaaat
    19741 cgacgctgaa ataagcataa agaataaaaa atgcgcggtc ggaaaattat tttaaatttc
    19801 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
    19861 acacgccgcc gggtcagcgg ggttctcctg agaattccgg cagagaaagc aaaaataaat
    19921 gcttgactct gtagcgggaa agcgtattat gcacaccccg cgccgctgag aaaagcgaag
    19981 cggcactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    20041 ttaacgtcgc aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac
    20101 gaagtttaat tc
//
LOCUS       DARNVM010000084        19835 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.084, whole genome shotgun sequence.
ACCESSION   DARNVM010000084 DARNVM010000000
VERSION     DARNVM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.084"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..443
                     /gene="dosC"
                     /locus_tag="SI621_002730"
     CDS             <1..443
                     /gene="dosC"
                     /locus_tag="SI621_002730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HEI4065424.1"
                     /translation="REIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQ
                     AFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIAL
                     SLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL"
     gene            468..2867
                     /gene="dosP"
                     /locus_tag="SI621_002731"
                     /gene_synonym="pdeO"
     CDS             468..2867
                     /gene="dosP"
                     /locus_tag="SI621_002731"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HEI4065425.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKIYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQNWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            3125..3706
                     /gene="ddpX"
                     /locus_tag="SI621_002732"
     CDS             3125..3706
                     /gene="ddpX"
                     /locus_tag="SI621_002732"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEI4065426.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            3720..5270
                     /locus_tag="SI621_002733"
     CDS             3720..5270
                     /locus_tag="SI621_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEI4065427.1"
                     /translation="MKRSIPFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            5272..6294
                     /locus_tag="SI621_002734"
     CDS             5272..6294
                     /locus_tag="SI621_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4065428.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            6291..7187
                     /locus_tag="SI621_002735"
     CDS             6291..7187
                     /locus_tag="SI621_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HEI4065429.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKCSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7184..8170
                     /locus_tag="SI621_002736"
     CDS             7184..8170
                     /locus_tag="SI621_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4065430.1"
                     /translation="MTHPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDALKAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPKSRREAKAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGTQCENVPALTACGDNNHRCACWYPQQEVISV"
     gene            8163..9089
                     /locus_tag="SI621_002737"
     CDS             8163..9089
                     /locus_tag="SI621_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4065431.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYICSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(9145..9576)
                     /gene="osmC"
                     /locus_tag="SI621_002738"
     CDS             complement(9145..9576)
                     /gene="osmC"
                     /locus_tag="SI621_002738"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HEI4065432.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDAATFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9921..10136
                     /gene="bdm"
                     /locus_tag="SI621_002739"
     CDS             9921..10136
                     /gene="bdm"
                     /locus_tag="SI621_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HEI4065433.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAELGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10238..10375
                     /gene="sra"
                     /locus_tag="SI621_002740"
     CDS             10238..10375
                     /gene="sra"
                     /locus_tag="SI621_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HEI4065434.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            10532..12229
                     /gene="maeA"
                     /locus_tag="SI621_002741"
     CDS             10532..12229
                     /gene="maeA"
                     /locus_tag="SI621_002741"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEI4065435.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            12363..13373
                     /gene="adhP"
                     /locus_tag="SI621_002742"
     CDS             12363..13373
                     /gene="adhP"
                     /locus_tag="SI621_002742"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEI4065436.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            13559..13837
                     /locus_tag="SI621_002743"
     CDS             13559..13837
                     /locus_tag="SI621_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4065437.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            13837..14121
                     /locus_tag="SI621_002744"
     CDS             13837..14121
                     /locus_tag="SI621_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEI4065438.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLRKFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(14172..14825)
                     /gene="fdnI"
                     /locus_tag="SI621_002745"
     CDS             complement(14172..14825)
                     /gene="fdnI"
                     /locus_tag="SI621_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HEI4065439.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(14818..15702)
                     /gene="fdnH"
                     /locus_tag="SI621_002746"
     CDS             complement(14818..15702)
                     /gene="fdnH"
                     /locus_tag="SI621_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HEI4065440.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(15715..18762)
                     /gene="fdnG"
                     /locus_tag="SI621_002747"
     CDS             complement(15715..18762)
                     /gene="fdnG"
                     /locus_tag="SI621_002747"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(18175..18177),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEI4065441.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            18994..>19835
                     /gene="yddG"
                     /locus_tag="SI621_002748"
     CDS             18994..>19835
                     /gene="yddG"
                     /locus_tag="SI621_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HEI4065442.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCG"
BASE COUNT         4804 a         5128 c         5085 g         4818 t
ORIGIN      
        1 aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg attattgacg
       61 ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat gaaattctgc
      121 gtaaagtctc tcaggccttt tatgacaacg tccgcagtag tgattatgtt ttccgctacg
      181 ggggcgatga atttatcatt gttttgactg aagcatctga aaacgaaacg ttacgtaccg
      241 cagaacgtat tcgcagtcgg gtagagaaaa ccaaactgaa agccgcgaac ggcgaagata
      301 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
      361 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
      421 aactctggaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
      481 atgcggataa tgccgccgat ggcatttttt tccccgccct tgagcaaaat atgatgggcg
      541 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
      601 gggggtacaa acgtgaagaa gttattggca ataacattga tatgctgatt ccgcgggatt
      661 tgcgtcccgc gcatcctgaa tacatccgtc acaaccgtga aggcggtaaa gcgcgcgtcg
      721 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
      781 ttgcgctatc gaaagtgagc gccgagggta aaatttatta cctggcgctg gtacgggatg
      841 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
      901 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
      961 ttaccgaaat gtttggttac tgcattagcg aagccagcgg tatgcagccc gatacactcc
     1021 tgaacattcc agaattccct gctgataacc gcattcgttt acaacagttg ctatggaaaa
     1081 ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac cggtgaaaaa atctggatta
     1141 aagcctctat cagcccggtc tatgatgtgc tcgcgcatct gcagaacctg gtaatgactt
     1201 tctcggatat caccgaagaa cggcaaatcc gccaacttga aggcaatatt ctcgccgcca
     1261 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     1321 ttctcaacga atcgcatgtt tcgctgttcg cgctgcgcaa cgggatgccg atacactggg
     1381 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaaa ctggtcagcg accattcgtc
     1441 agcgtgatgg tgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     1501 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     1561 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     1621 caaatcgcaa taacctgcac aattacctcg atgacctggt cgacaaagcc gtctctccgg
     1681 tggtgtatct catcggtgtt gaccatattc aagatgtgat tgatagcctt ggctatgcgt
     1741 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     1801 agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt gagcctggaa aacgacgtca
     1861 gtaacataac ccaaatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     1921 acgataaacc attcccgctc accttgagca ttggcatcag ctacgacgtg ggtaaaaacc
     1981 gcgattacct gctctccact gctcacaatg caatggatta tattcgcaag aatggcggta
     2041 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     2101 gcgcagcgct gaaagaagcg attagcaata accaactaaa actggtttac cagccgcaaa
     2161 tcttcgcaga aacgggtgaa ctctacggca tcgaagccct tgctcgctgg cacgatcccc
     2221 tgcatggtca tgtgccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     2281 aaaatattgg acgctgggtc atcgcggaag cttgtcgtca gttagcagaa tggcgtagcc
     2341 agaatattca tatcccggcg ctttccgtta acttgtcggc gctgcacttt cgcagtaatc
     2401 aactgcctaa tcaggtgtct gatgcaatgc acgcctgggg tattgacggc caccagctga
     2461 cggtggaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     2521 agatcctgcg cgatatgggc gtgggtttat cggtagatga ttttggcacg ggcttttccg
     2581 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgacaaa agttttgtcg
     2641 atcgttgtct gaccgaaaaa cgcatccttg ccttacttga agccattacc agcattgggc
     2701 aaagccttaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     2761 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctgcccgccg
     2821 aagaaattcc aggctggatg agcagtgtgt taccgctgaa aatctgacaa attcctctcg
     2881 cccgcactcg cgggttttca tttaacgtga cactgtcact taaatactgt gatttcagct
     2941 acgattccgg gagattcctt ccttaacttc ccctcttgca ccaaaactgc actacgctgc
     3001 acaaatgttg atcaaaagtt aaaaaatata actttaagcc agcattctgg cgcttatccc
     3061 ggcatggcat gagatctgca taagcggaaa gcgcagcaat ttttgtctta tacaggaacc
     3121 gtttatgtcg gataccaccg aactggttga tttagccgtg atcttccctg atctggagat
     3181 cgaactgaaa tacgcctgcg ctgataacat cacaggcaaa gctatttatc agcaagcgcg
     3241 ttgtctgtta cacaaggatg cgattactgc gctggcgaaa agtattagta tcgcccagct
     3301 gtcaggctta caactggtga tttacgatgc gtatcgccca caacaagcac aggcgatgtt
     3361 gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag
     3421 ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac gggaacatcc tggatatggg
     3481 tgcagagttc gatgaaatgc acgaacgttc ccatgcttat cacccttccg tcccgccagc
     3541 cgctcagcgc aatcggctgt tgctgaatgc gattatgact ggcggtggct ttgtcggtat
     3601 cccgagcgaa tggtggcact tcgaattacc ccaggcagcg agttacccac tgctcgctga
     3661 tcaattcacc tgttttatat ccccaggcac acagcacgcc agttaatcag gaaactacca
     3721 tgaagagatc gataccgttt cgtcctacat tgctcgcgat cgtccttgcc acaacaatgc
     3781 cggttgcgca cgccgccgta ccgaaagata tgctggtgat cggtaaagct gccgacccac
     3841 aaaccctcga cccagcggta acaattgata ataacgactg gacagtgacc tacccgtctt
     3901 atcagcgact ggttcagtac aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg
     3961 atctggcaag tagctggaaa gcgtctgacg atcaaaaaga gtggacgttc accctgaagg
     4021 acaatgctaa atttgccgat ggcacacctg tcactgccga agcagtaaaa ctctcttttg
     4081 agcggttact aaaaatcggc caggggccag cagaagcatt tcccaaagat ttaaagattg
     4141 atgctcccga cgaacatacg gtgaagttta cccttagcca gccattcgca ccgttcctct
     4201 atacgctggc gaatgacggt gcatccatta tcaatccggc ggtcttaaag gaacatgcgg
     4261 ccgatgatgc ccgtggtttc ctcgcgcaaa ataccgctgg ctccggaccg tttatgctga
     4321 aaagctggca aaaaggtcag caattagttc tggtgccaaa tccgcattac tccggcaata
     4381 aaccgaactt taagcgagta tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc
     4441 agctctcccg tggcgacatt gacattgccg atgcgctgcc ggtggatcaa ctcaacgcac
     4501 tgaagcagga aaacaaagtc aacgtggcag agtatccgtc actgcgcgtc acctatctgt
     4561 atctgaataa cagcaaagcg ccacttaatc aggcggatct gcgtcgggcc atttcctggt
     4621 ctaccgatta tcagggtatg gttaacggca ttctgagtgg taacggaaaa caaatgcgcg
     4681 gcccgattcc ggaaggcatg tggggatacg atgcgacggc aatgcaatac aaccatgacg
     4741 aaacgaaagc caaagccgaa tgggataaag tgacgagcaa acccaccagc ctgacgtttc
     4801 tctactccga taacgacccg aactgggaac cgatcgctct ggcgacacaa tccagtctca
     4861 acaagctggg catcaatgtg aagctggaaa aactggcgaa cgccaccatg cgcgacagag
     4921 tgggtaaagg tgattacgac attgcgattg gcaactggag cccggatttt gccgacccgt
     4981 atatgtttat gaattactgg tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt
     5041 tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc gctagcgacc accgaccaga
     5101 cgcagcgtac ccgggactac cagcaggcac agaaaatcgt cattgatgac gctgcttatg
     5161 tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa agaggtgaaa ggttttgtgt
     5221 tcaatcccat gctggaacag gtcttcaata tcaataccat gagtaaataa cgtgaccttc
     5281 tggagtattt tacgccaacg ctgctggggg ctggtgctcg tggtggcggg cgtctgtgtg
     5341 ataacgttta ttatctcgca cctgatcccc ggcgatccgg cgcggttact ggcgggtgac
     5401 cgcgccagcg atgctatcgt ggataatatt cgccagcaac tgggactgga ccagccactg
     5461 tacgtacagt tttaccgcta cgtcagcgac ctgtttcagg gtgacctggg aacatccatt
     5521 cgtaccgggc gtccggtgct ggaagagttg cgtatatttt tcccggcgac gctggaactg
     5581 gctttttgtg ccctgctgct ggcactcctg attggcatcc cgctgggcat actctctgca
     5641 gtctggcgaa atcgctggct ggatcatctg gtgcgaataa tggccattac cggaatctcc
     5701 acacctgcgt tctggcttgg gctgggcgtc attgtgctgt tttatggtca tctgcaaatt
     5761 cttcccggcg gcggaaggct tgatgactgg ctggatccac caacgcacgt taccggcttt
     5821 tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct tcttcaatgc gctgcaacat
     5881 ctcatcttac cggcattaac gctggcgttc gttcacctgg gaattgtcgc tcgccagatc
     5941 cgctcagcta tgctggaaca attgagtgaa gattacattc gtaccgcccg ggccagcggc
     6001 ttgcccggct ggtatatcgt tttatgttat gcgctaccca atgcgttgat cccatcgatt
     6061 accgtattgg gtctggcgct gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt
     6121 tttgcctggc ccggaatggg tgcatgggta gtaacatcaa tacaggcgct cgacttcccg
     6181 gcagtgatgg gctttgccgt cgtggtttca tttgcttatg tgctggtcaa cctggtggtg
     6241 gatttgctct atttgtggat tgatccgcgt atcggacgtg gaggtggtga atgatgctaa
     6301 gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg
     6361 tatggatgct gaaatgcagt ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc
     6421 tactgatgat gattttttca ccgtggctgg cgacgcatga tcccaacgcc attgatttaa
     6481 ccgcccgcct tttgccgcct tctgcggcgc actggtttgg caccgatgaa gtgggacgcg
     6541 atctgtttag ccgcgtactg gtcggcagtc agcaatcaat tctcgccgga ttagtggtgg
     6601 tcgccattgc aggtatgatg gggtcgctac tcgggtgtct atccggtgtg cttggcggac
     6661 gcgcagacgc gattatcatg cgcatcatgg acattatgct gtcgattcct tcgctggtgc
     6721 tgacaatggc actggcagcc gctctcgggc cgagtttgtt taacgccatg ctggcgattg
     6781 ctatcgtgcg tatcccgttt tatgtgcgcc tggcgcgggg gcaaacatta gttgtccgcc
     6841 agtacactta cgttcaggcg gcgaaaacct ttggtgcgtc acgctggcat ctgatcaatt
     6901 ggcatatttt acgtaactcc ctgccgccgc tgattgtgca ggcatcgctg gatatcggta
     6961 gcgcgatttt aatggccgcc acgttgggat ttattggcct gggtgctcaa caaccgagtg
     7021 ctgaatgggg ggcgatggtg gcgaatggtc gcaactatgt gctcgatcaa tggtggtatt
     7081 gcgcatttcc gggggcagcg attttgctta ccgccgtcgg gttcaatctc tttggtgatg
     7141 gtattcgcga tctgcttgac ccgaaagcag gaggaaagca gtcatgaccc accccgttct
     7201 ggacattcaa cagctgcatt tgagtttccc cggttttaac ggcgacgttc acgcgctcaa
     7261 caatgtgtcc ttgcagatta accgcggtga aattgtcggt ctggtgggag aatccggctc
     7321 gggtaaatca gtcaccgcaa tgctgattat gcgtctgcta ccaacgggca gttattgcgt
     7381 acatcgggga catatttcac tgctgggaga agatgctctt aaagctcggg aaaagcagct
     7441 tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag gaaccgatga ccgcccttaa
     7501 tccgacacgt cgaataggtc ttcagatgat ggacgtgatc cgccatcatc aaccaaaaag
     7561 tcgtcgggaa gccaaagcta aagcgattgc cctgctggaa gagatgcaaa tcccggatgc
     7621 cgtggaagtt atgtcgcgct atccgtttga gctttcaggt ggtatgcgac agcgggtaat
     7681 gattgcgctg gcattctcct gcgagccgca attgattatt gccgacgaac cgactacggc
     7741 gctggacgtc acggtacagt tgcaggtact gcgtctgctt aaacataaag cccgcgccag
     7801 tggaactgcg gtactgttca tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag
     7861 cgtttacgtg atgtatgccg gaagcgtgat cgagagcggc gtgacggcag acgttatcca
     7921 tcatccccgg catccgtata ccattggttt gctgcaatgc gcaccggaac atggaatacc
     7981 gcgccagcca ttacccgcca ttccagggac ggtaccaaac ctcacccatt tgcctgacgg
     8041 ctgcgctttt cgcgatcgtt gctatgcggc aggtacacag tgtgaaaacg tcccggcgct
     8101 gacagcgtgt ggtgacaaca accaccgctg cgcctgttgg tatcctcagc aggaggtcat
     8161 tagtgtctga cacgttatta acgttacgcg acgtccatat caatttcccg gcccgaaaaa
     8221 actggcttgg taaaactacg gaacatgttc atgccatcaa tggtattgat ttacagatcc
     8281 gtcgtggtga aaccttaggg atcgtcggtg agtcaggctg cggcaaaagc accctcgcac
     8341 agcttttaat gggtatgctg caaccgagcc acgggcagta catctgttca ggatcacaac
     8401 gcattatgca gatggtgttt caggacccgc tctcttcgct taatccgcgc ttaccggtgt
     8461 ggcgcatcat cacggaaccg ctctggattg ctaagcgtag tagtgaacaa cagcggcgag
     8521 cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc ggagtatctc gaccgcttgc
     8581 ctcatgcgtt ctcaggcggg cagcggcaac gtatcgccat tgccagagca ctctcttcgc
     8641 agcctgacgt gattgtgctt gatgagccaa cctctgcgct ggatatctct gtgcaggcgc
     8701 agatcctcaa tttactggta acgctacagg aaaatcgcga gctgacttat gtgctgattt
     8761 cacacaatgt ctccgtgata cgtcatatga gcgatcgggt ggcggtgatg tatctcggac
     8821 agattgtgga actaggagac gcgcaacagg tgctgacagc tccggcacat ccatacaccc
     8881 gattattgct ggattccctc cccgccattg ataaaccgct ggaggaagaa tgggcattac
     8941 gtaaaacgga tctaccagga aaccgcacgt tgccacaagg ctgttttttc cgcgaacgct
     9001 gccctctggc aacccacgga tgtgaagtcc ggcaatcatt aacaataagg gaggacggac
     9061 gtgagatccg gtgctggcgg gcgctgtaga gacgctaaca acgcatcagg caatcacgcg
     9121 cctgacgcgt catacggcaa cgctttatga tttcaactgg taatcaagcg taatttcagc
     9181 tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc tggataatgc cgtcaaaggt
     9241 ggctgcatca atacccggca ccgcaacttc gctcttcagt gcgattttag taatcgcaaa
     9301 accggcatcc actttatcca gcgacacatc ggcggtggta tcaatcgatg ttggcgtgaa
     9361 tcccgcttcc cccagcatta atgaaagcgc cattgagaaa catgcggcat gcgctgcgcc
     9421 aatcagttct tcagggttgg ttcctttttc gccttcaaaa cgcgtgttaa atccatacgg
     9481 ctgttggttc agcacgccac tctcggtgga tactgttccc ttcccgcgtt tgatatcgcc
     9541 ttcccagtgt gcctgacctt tcttatggat tgtcattgtt gctctcctgt gggcttaaaa
     9601 aggaaattat agactacgtg agaaacagca cgaggataag caggatattc cgaataaaat
     9661 ccggcgaaaa cattatctct gtacttaaat taaattccca caatagataa cggggaatat
     9721 ataatccaaa agatattgta tcaatattaa tcggatcaca ccaattacaa tccctatcaa
     9781 tatcatagtg ttaaaatata aagctcatca accaggacta atcttaacaa cgaagcggca
     9841 aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac accataaata
     9901 cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca acagcagaac
     9961 ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gctcggtgtt gtcactgaag
    10021 atgacattct catggagctg accaaatggg tggaagcttc tgataacgac atcctcagtg
    10081 atatctacca gcaaacgatc aattatgtgg tcagtggcca gcaccctacg ctttaaggtg
    10141 ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc tacggcgatg
    10201 ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc gtcaggcacg
    10261 tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta ccgaaggtga
    10321 caaatccagc gtggtaaata acccaaccgg tagaaaacgc cccgctgaaa agtaattcat
    10381 aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt ttttgttttt
    10441 atctgcttta tacttggggc cgacgccctg gcggtaaagc aaagacgata aaagcacccc
    10501 agggatggat attcaaaaaa gagtgagtga catggaacca aaaacaaaaa aacagcgttc
    10561 gctttatatt ccttacgctg gccctgtact gctggaattt ccgttgttga ataaaggcag
    10621 tgccttcagc atggaagaac gccgtaactt caacctgctg gggttactgc cggaagtggt
    10681 cgaaaccatt gaagaacaag cggaacgagc atggatccag tatcagggat tcaaaaccga
    10741 aatcgacaaa cacatctacc tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg
    10801 tctggtaaac aatcatcttg atgagatgat gcctgttatt tataccccaa ccgtcggcgc
    10861 agcctgtgag cgtttttctg agatctaccg ccgttcacgc ggcgtgttta tctcttacca
    10921 gaaccgccac aatatggacg atattctgca aaacgtgccg aaccataata ttaaagtgat
    10981 tgtggtgact gacggtgaac gtattctggg gcttggtgac cagggcatcg gcgggatggg
    11041 cattccgatc ggtaaactgt cgctctatac cgcttgtggc ggcatcagcc cggcgtatac
    11101 ccttccggtg gtgctggatg tcgggacgaa taaccaacag ctgcttaacg atccgctgta
    11161 tatgggctgg cgtaatccgc gtatcactga cgacgaatac tatgaattcg ttgatgaatt
    11221 tatccaggcg gtgaagcaac gctggccgga cgtgctgttg cagtttgaag acttcgcaca
    11281 aaaaaatgcg atgccgttac ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga
    11341 cattcagggc actgcggcgg taacagtcgg cacactgatc gcagccagcc gcgcagcagg
    11401 tggtcagtta agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat
    11461 tgccgaaatg atcatcgccc agactcagcg tgaaggatta agcgaggaag cggcgcggca
    11521 gaaagtcttt atggttgatc gctttggcct gctgaccgac aagatgccga acctgctgcc
    11581 tttccagacc aaactggtgc agaaacgcga aaacctcagt gactgggata ccgacagcga
    11641 tgtgctgtca ctgctggatg tagtgcgtaa tgtaaaacca gatattctga ttggcgtctc
    11701 aggacagacc gggctgttta cggaagagat catccgtgag atgcataaac actgtccgcg
    11761 tccgatcgtg atgccgctgt ctaacccgac gtcacgcgtg gaagccacac cgcaggacat
    11821 tatcgcctgg actgaaggta acgcgctggt cgccactggc agtccgttta acccagtggt
    11881 atggaaagat aaaatctacc ctatcgccca gtgtaacaac gcctttattt tcccgggcat
    11941 cgggctgggc gttattgctt ccggcgcgtc acgtatcacc gatgagatgc tgatgtcggc
    12001 aagtgaaacg cttgctcagt attcgccgct ggtcctgaac ggcgaaggtc tggtactacc
    12061 ggaactgaaa gatattcaga aagtctcccg cgcaattgcc tttgcggttg gcaaaatggc
    12121 gcagcagcaa ggcgtggcgg tgaaaacctc cgccgaagct ctgcaacagg ccattgatga
    12181 taatttctgg cacgccgaat accgcgacta ccgccgtacc tccatctaag cctgcgcccg
    12241 gtggtgaaag ctgccgggca attttcctct ccctttttca gatctcatcc atactgggta
    12301 gtggcgaata aatctcattt gcctcacctg ctatgcagaa catcatccga aaaggaggaa
    12361 ctatgaaggc tgcagttgtt acgaaggatc atcatgttga cgttacggat aaaacactgc
    12421 gctcactgaa acatggcgaa gccctgctga aaatggagtg ttgtggtgta tgtcataccg
    12481 atcttcatgt taagaatggc gattttggtg acaaaaccgg cgtaattctg ggccatgaag
    12541 gcatcggtgt ggtggcagaa gttggcccag gtgtcacctc attaaaacca ggcgatcgtg
    12601 ccagcgtggc gtggttttac gaaggatgcg gtcattgcga atactgtaac agtggtaacg
    12661 aaacgctctg ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg atggcggaag
    12721 agtgcatcgt ggtggcggat tacgcggtaa aagtgccaga tggcctggac tcggcggcgg
    12781 ccagcagcat tacctgtgca ggggtcacca cctacaaagc cgttaagctg tcaaaaattc
    12841 gtccagggca gtggattgct atctacggtc ttggcggtct gggtaacctc gccctgcaat
    12901 acgcgaagaa tgtctttaac gcgaaagtga tcgccattga tgtcaatgat gagcagttaa
    12961 aactggcaac cgaaatgggc gctgatttag cgattaactc acgcactgaa gacgccgcca
    13021 aaattgtgca agaaaaaacc ggtggcgctc acgccgcggt ggtgacagcg gtagctaaag
    13081 ctgcgtttaa ctcggcggtt gatgctgtac gagcaggcgg tcgtgttgtg gctgtcggtc
    13141 tgccgccgga gtctatgagc ctggacatcc cacgccttgt gctggatggt attgaagtgg
    13201 tcggttcgct ggtcggcacg cgccaggatc tcaccgaagc cttccagttt gcggccgaag
    13261 gcaaagtggt gccgaaagtc gccctgcgtc cgttagcgga catcaacacc atctttactg
    13321 agatggaaga aggcaaaatc cgtggccgta tggtgattga tttccgccgc taagaggcct
    13381 ttgctgcgac tgccatgttc gggtcgcagc ctgttttgcg agacgctttc cgcaatttca
    13441 ctcttcttct tttcctcccc catctttccc ctcacaattc gcgaaaacgt agcgaaattt
    13501 gttgcgctgc acggaacaat agtgatagtg ttcctttcga aggaacaagg atgaagagat
    13561 gataatgaat ttcagacaca agggattgcg tgacttgttt cttctcggca aaacctccgg
    13621 cgttataccg acacaagtca aacgattacg ccaccgactc gctgtgattg atgcagcaag
    13681 ctgccttgct gatatcgata tgcccggtta ccgactacac ccattaagcg gtgatcgcga
    13741 tggaatttgg gcgatatctg tctcgggcaa ctggcgaatc acatttgaat tcgtcaatgg
    13801 cgatgcatac atactggatt acgaggacta tcactaatga aaatggccaa tcatccccgc
    13861 ccgggggaca ttattcagga atcactggac gaacttaatg tcagcctgcg caagtttgcc
    13921 agagcaatgg aaattgcgcc ctcaacggca agccgattgc tgaccggaaa agcagctttg
    13981 acaccagaaa tggcgattaa actttccgtg gtgatcggca gttcgccgca aatgtggctg
    14041 aatctgcaaa acgcctggag tctggcagag gcagaaaaaa cggtggatgt gtcgcgatta
    14101 cgccgtctgg ttacgcaata aaaaaatggc gcttcttaaa gcgccatttt cagcttaaag
    14161 ttcaaaagag attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca
    14221 cgataccagc gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc
    14281 cctttaatcg atcctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc
    14341 aggatgatac ccgcagccgc gtgaatcagc aggctgtagc gaacaacctg catcgggaag
    14401 tactgcgcaa agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg
    14461 ctcatgatcg accagaacat catcttttgc ccggcgttgt acttaccaac atccgccact
    14521 ttgtgctcat tgccttttaa tacttcgaca atgttcaaca gccacggaat atctttctta
    14581 tccgggatgt tgtgatgcac aaaacgcaca aacataaaca tcagcgcgac gaaaatcgca
    14641 atgccgaaga acgggtgcaa aatgcgcccc atctgcggcg taccgaaggt ttgcgtcagc
    14701 cattgcagcg tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc
    14761 accacggtcc agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta
    14821 ctcatgatga tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtagtg
    14881 gaaaatcaac ccggcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc
    14941 tttccacagg cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc
    15001 cggctgatcg gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt
    15061 gtagacgcca gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct gttccgccag
    15121 ctccagcatc tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca
    15181 agccggttcc tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt
    15241 atcctctttg ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc
    15301 gatgcagttt tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccagc
    15361 agacgggcac gccttcaggc agcccggatc ttcacagtgc atacagccgt ctttacggat
    15421 cagccactcc agcttgccgt tctgttcggt ttcgctaaag cgcatcaccg tccaggactt
    15481 ggcgctcaga tcggcggggt tatcgtaaac cccgacgcag tgccccactt catcacggat
    15541 gtcgttccac tccgaacacg ccacctgaca ggctttacaa ccgatacagg tggaaacgtc
    15601 gataagtttt gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt
    15661 agttgcggac cttttgataa tgtcctgcgt ttccatagcc atctgttcgc ccccttacgc
    15721 cttctcgatg ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac
    15781 attcggcgtc agagtgttag cgatataacc tttacgcgcg acaccctcaa agccccagtg
    15841 gattggaata cccaccgttt caacctgctg accgtttaca ttcagcggtt tcagacggcg
    15901 cgttaccacc gccaccgcgc ggataaagcc acgcttgctt gagacagtga cacgatcgcc
    15961 attattaatg cctttcgccg ccgccagcgt ttcgctgatt tccacaaact gttccggctg
    16021 agcaatggcg ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata
    16081 ggtcgtaccc acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata
    16141 cagacgaacc accgggttag acaccacgtt cggatgcagc gggttagtgc ccagcggcgt
    16201 ttcgattggc tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag
    16261 gcgtcccatc ccttccggct gcatgataaa cggcccggtt ggcgtacccg gtgcggcatt
    16321 gccgaagtcc ggaatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg
    16381 tttcggatcc cacggtttac cgttgatatc cgccgaagca cggttgtaga gtacgcgacg
    16441 gttgagcggc cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt
    16501 atcgcggtta gccatctggt tgccctgctc tgtccagcta ccggtgtaga tccagcaaga
    16561 agatgcggtt gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gccctttctt
    16621 cgcaatcagc acaccattag catcatagag atcttccagc gcatagccgt tgttctcttt
    16681 agctacttcg tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag
    16741 cggttctacg cctttaccac cttcggcctg gtacagctcg cgcagatgat ggtagatacc
    16801 cgccagaatt tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca
    16861 ctgcaaccag cgaccggagt tagcaataga accatcttcc tcagcaaagc aggtcgaagg
    16921 cagacggaat acttcagtct gaatagacgc aggatcgaca tcgttcgact cgccgtggtt
    16981 ctgccagaag gtagaagttt cagtcaccag cggatcgata accaccatat acttcagctt
    17041 gctcaggcag cttaccactt tgtttttgtc cgggaaggac gcaaccgggt taaagccctg
    17101 gcagaaataa ccggtgactt tgccttcatc catcatgttg aaatacttga tgacgtcgta
    17161 ggtctggtcc cacttgggca gccagtcgta gccccagttg ttctctttct gcgcagcatc
    17221 gccgtagaaa gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac
    17281 ctgatcagcc agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg
    17341 tttttctgac ggcagcgtca gataacccgg caggctggta gagagcaggc ctaagtcggt
    17401 cagcccctga atgttggagt gaccacgcaa tgcgttcacg ccgccaccgg ccatacccat
    17461 gttaccgagc agtaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg
    17521 ctgcgtccag cccagcgcgt aaaggaaggt ggttgtgcga tccggtgcgc tggtggaggc
    17581 cagcacttca cacactttca ggaagtcggc ttttggcgta ccacagatgt tttctactac
    17641 gtccggcgtg tagcgggaaa cgtgctcttt cagcaagttc cacacacagc gcggatgagt
    17701 cagtgtttca tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc aggacgattt
    17761 atcgtattga cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga aagcaaaatc
    17821 atcacgcacc agcagactgg cgttggtgta atgcttaacg tattcggcgt tgattttgtt
    17881 gttttcgatc aggtagcgca aaacgccaga caggaacgta atgtccgtac cggaacgaat
    17941 aggtgcgtaa atatccgcta cagaagcggt acgcgtaaaa cggggatcga caacgatcaa
    18001 ggttgcatcg ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc
    18061 agcagcgtta ccgcccatca ccattacgac gttagcgttt ttgatatcca cccagtggtt
    18121 ggtcatcgca ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac
    18181 gcgcgcctgg ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat
    18241 cccggtttcg ttgcttgcac cggaggcaca cagcataccg gtagaaagcc aacggtttac
    18301 cgttacgccc tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt
    18361 cgcaatacgg gagaatgctt catcccagct aatgcgctgc catttgtcag aacctggcgc
    18421 acgatattcc gggtagcgca gacggttttc gctgttgacg taatccagca aaccggcccc
    18481 tttcgggcat aacgcaccac ggcttaccgg atgatccggg tcaccttcaa tgtgataaat
    18541 cgcttctctg gcgtttttcg cgccatcacc caggctatac atcaatagcc cgcaacctac
    18601 ggaacagtat gtgcaggtgt tacggatctc tttagcgcgt aataatttat agtttcgcgc
    18661 ctgagccagt gcttgcttcg gggcaaagcc caatgctgct actgttgttc ccgccatacc
    18721 gcccgcgcag attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca
    18781 ggggtattac tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa
    18841 aaagaacacg tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg
    18901 gtagaaaaac gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta
    18961 atttttaacg gcaagagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata
    19021 gggctaatag cgatcgtcct gtggagcacg atggtaggat tgattcgcgg tgtcagtgag
    19081 gggctcggcc cggtcggcgg cgcggctgct atctattcat taagcgggct gctgttaatc
    19141 ttcacggttg gatttccgcg tattcggcag attccgaaag gctatttact cgccgggtgt
    19201 ctgttattcg tcagctatga aatctgtctg gcgctttcct taggatatgc ggcgacccgt
    19261 catcaggcga ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt
    19321 gccattctgt ttaatggtca gaaaaccaac tggctgattg taccaggatt attattagcc
    19381 ctggttggcg tctgctgggt gttaggtggt gacaatgggt tacattatga tgaaatcatc
    19441 aataatatca ccaccagccc attgagttat ttcctggcgt ttattggtgc gtttatctgg
    19501 gcagcctact gcacagtaac gaataaatac gcacgcggat ttaacggaat taccgttttt
    19561 gtcctgctaa cgggagcaag tctgtgggtt tactattttc tcacgccaca accagaaatg
    19621 gtatttagca cgccagtcat gattaaactc atctctgcgg catttacctt aggatttgct
    19681 tatgctgcat ggaacgtcgg tatattacat ggcaacgtca ccattatggc ggtaggttcg
    19741 tattttacgc ccgtcctttc ctcagcgctt gcagccgtgc tgctcagcgc cccgctgtcg
    19801 ttctcgttct ggcaaggcgc gctaatggtc tgcgg
//
LOCUS       DARNVM010000085        19713 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.085, whole genome shotgun sequence.
ACCESSION   DARNVM010000085 DARNVM010000000
VERSION     DARNVM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.085"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..908
                     /locus_tag="SI621_002749"
     CDS             <1..908
                     /locus_tag="SI621_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEI4065443.1"
                     /translation="KVTLKLAHNLERSHVVHQSFEELAKEVKQLSKGNMVIRIYPSSQ
                     MGNARETMELLQNGALDMTKGSTSDLESFDNIYAIYNLPFLFKDHAHFNKVVFGEVGK
                     EIMDSTKDKGFFALSAYVAGTRSFYAKKPITKPEDLKGLKIRVQPSPTTIKMIELMGG
                     SPTPISFGEVYTAMQQGVVDGAENNVPSWVQTRHIEIAKVFSEDEHASIPDFLVISTK
                     TWNKLTPEQQQILETAAKKSEAYQQKLWEKIDADTRAQAKAMGGEIVKVDKAPFRAAV
                     QPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            953..1456
                     /locus_tag="SI621_002750"
     CDS             953..1456
                     /locus_tag="SI621_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEI4065444.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINILILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            1456..2757
                     /locus_tag="SI621_002751"
     CDS             1456..2757
                     /locus_tag="SI621_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEI4065445.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(2813..3820)
                     /locus_tag="SI621_002752"
     CDS             complement(2813..3820)
                     /locus_tag="SI621_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HEI4065446.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGESPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(3937..4983)
                     /gene="ansB"
                     /locus_tag="SI621_002753"
     CDS             complement(3937..4983)
                     /gene="ansB"
                     /locus_tag="SI621_002753"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HEI4065447.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMSDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDTKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(5159..5878)
                     /locus_tag="SI621_002754"
     CDS             complement(5159..5878)
                     /locus_tag="SI621_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HEI4065448.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQSEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(6062..6388)
                     /locus_tag="SI621_002755"
     CDS             complement(6062..6388)
                     /locus_tag="SI621_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEI4065449.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            complement(6388..7107)
                     /gene="trmB"
                     /locus_tag="SI621_002756"
     CDS             complement(6388..7107)
                     /gene="trmB"
                     /locus_tag="SI621_002756"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEI4065450.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            7268..8320
                     /gene="mutY"
                     /locus_tag="SI621_002757"
     CDS             7268..8320
                     /gene="mutY"
                     /locus_tag="SI621_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEI4065451.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGATLSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGSLYCFPQFADEESLRLWLTQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8348..8623
                     /locus_tag="SI621_002758"
     CDS             8348..8623
                     /locus_tag="SI621_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEI4065452.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            8688..9767
                     /gene="mltC"
                     /locus_tag="SI621_002759"
     CDS             8688..9767
                     /gene="mltC"
                     /locus_tag="SI621_002759"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HEI4065453.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSITINMVPNHLDKRAHKYLGMVRQASRKYGIDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            9969..11225
                     /gene="nupG"
                     /locus_tag="SI621_002760"
     CDS             9969..11225
                     /gene="nupG"
                     /locus_tag="SI621_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HEI4065454.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11271..13406)
                     /gene="speC"
                     /locus_tag="SI621_002761"
     CDS             complement(11271..13406)
                     /gene="speC"
                     /locus_tag="SI621_002761"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEI4065455.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILAHCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            13798..14505
                     /locus_tag="SI621_002762"
     CDS             13798..14505
                     /locus_tag="SI621_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEI4065456.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14611..14686
                     /locus_tag="SI621_002763"
     tRNA            14611..14686
                     /locus_tag="SI621_002763"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14644..14646,aa:Phe,seq:gaa)
     gene            14884..16146
                     /locus_tag="SI621_002764"
     CDS             14884..16146
                     /locus_tag="SI621_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEI4065457.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVIKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYSRWSEIDIDNAMWTIPAEREPLPGVKYSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLGANREQCISPFEYAKINNPLK"
     gene            16397..17924
                     /locus_tag="SI621_002765"
                     /pseudo
     CDS             16397..17924
                     /locus_tag="SI621_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(17983..18401)
                     /locus_tag="SI621_002766"
                     /pseudo
     CDS             complement(17983..18401)
                     /locus_tag="SI621_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19202..19702
                     /locus_tag="SI621_002767"
     CDS             19202..19702
                     /locus_tag="SI621_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEI4065458.1"
                     /translation="MENIHSLEKIIKYQQNKDPLYPCQEHLLIQLCIRANKKIQDNIS
                     RMLGGYGINYSTYMVLTVLSTADNHCLSPSEISRELQFTRTNITRITDFLEKAGYTER
                     TDSKEDRRAKKISLTSEGLSFIQGITLTQNQYLKEIWSPLTYDERELFKDINQKLLTH
                     FADVRS"
BASE COUNT         5032 a         4875 c         4647 g         5159 t
ORIGIN      
        1 aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta catcagagtt
       61 ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg atccgtattt
      121 atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa aatggcgcac
      181 tggatatgac caaaggttcc accagtgatc tggagtcttt tgataatatt tatgctatct
      241 ataatttgcc gttcttattt aaagatcatg ctcacttcaa caaagttgtt tttggtgaag
      301 ttggtaaaga aattatggac tcaacaaaag acaaaggctt ttttgcttta tcagcttatg
      361 tagcgggtac acgcagtttc tatgcgaaaa aaccgattac taagccagaa gatcttaaag
      421 ggctgaaaat tcgtgttcag ccaagcccga ccaccatcaa aatgattgaa ctgatgggtg
      481 gctcacccac tccaatttca tttggcgaag tttatactgc gatgcagcaa ggtgtagtag
      541 atggcgcaga aaataacgtg ccttcctggg tgcagactcg acatatcgaa attgccaaag
      601 tcttttctga agatgaacat gcctcaattc ctgacttttt ggttatctcc accaaaacat
      661 ggaataaatt aaccccagaa cagcaacaaa tcctcgaaac agcggcgaag aaatccgaag
      721 cttatcaaca aaaattgtgg gaaaaaattg acgctgacac acgtgctcag gcgaaagcta
      781 tgggtggtga aatcgttaag gttgataaag ccccattccg tgccgcagtt caacctctgt
      841 ttgacgactt caaaaaagat ccaaaacaag ctgctttgtt agagaaattc gataacgctg
      901 ctcaataata tttcgggcca gtctctggcc ctgattttcc aggatgctta caatgatatt
      961 taaccgcctg aaacttgcgg tagaccgcgt cattgccgcg ttttctgtcg ccgtcatgct
     1021 tgctctggtt gtatgcgtag tctggcaagt atttagccgc tacgtactta accagccaag
     1081 tacattaacc gatgagctgg cacgcttttt gatgatctgg gtaggattat taggtgctgc
     1141 atataccgtc ggcgcgcaga gacatctctc tatcgattta tttgctctcg ccctgaataa
     1201 gcgcaagcaa ttacttctta gtatagtcat taacattctc attttaggtt ttgccggttc
     1261 ggtcattgtg actggtggcc tgaaattaat tgataaaacg ctggcaacat cacaagtatc
     1321 tgccgctatg caaataccga tgggatacgt atatatcatt ctgccattaa gtggagtggt
     1381 gatgatgttt tatgccttgt gttttattaa ccaaagcatt caacaactga aacaacctgc
     1441 gcaggaggcc agttaatgga tagttatatc gcgattacac tgtttggctg tttttttgtg
     1501 ctggtcttta ttggtgtgcc catttcgttc tcaatcggta ttgccactgt agcttcaatg
     1561 cttttgatgt ttccctggga tatcgcggca atcactgttt cgcaacgact ggctaatgga
     1621 ctggataact ttgccctgtt ggcaatcccc tttttcatct ttgccggaac gctgatgaac
     1681 agcggcggta ttgcgatacg tttgattaac ctcgcccagg taatggttgg ccgcgttcct
     1741 ggttcgcttg gtcatgtgaa cgtcctggcg aatatgatgt ttggttccat ttctggttca
     1801 gccgttgcgg cagctgctgc cgttggtggc acactgaatc ccattcagac caaagaaggt
     1861 tatgatccgg cattttctac cgctgtaaac gtctcatctt gtattactgg attactgata
     1921 ccgccctcaa acgtgttaat tgtgttttcc ctgacagctg gcggcgtttc tgtagcatcg
     1981 ctatttatgg cgggatattt gccagggatc ttaatgggtc tcgcggtaat gatagtctgc
     2041 ggcattattg ctaaacgccg tggctaccca ctttcagaaa gagcaacctt tgctcaggca
     2101 tgtaaagcgt ttctggatgc cttaccaagt ttgcttctgg tgttcattgt aatgggcggc
     2161 attctgggcg gcatttttac cgcaactgaa gcgtcagcaa ttgccgttgt ctacacattt
     2221 attctctcgg tgctgattta ccgggaagta aaatggcgcg atttacccaa actgattctg
     2281 gaatcggttg ttaccacttc gattgtatta ctgttgatcg gtttttccgt gggcatgtcc
     2341 tgggcaatga ccaatgctga tattccttat atgatcagcg acgcattgat ggggatttct
     2401 gataacccac tgatcatatt gctgctgatt aatatcgtct tattaattgt cggcatcttt
     2461 atggacatga caccggctgt actaatcttc acaccaatct tcttgccgat tgcacaagag
     2521 ctgggaatgg atccggtaca tttcggtatt atgatggtgg ctaacctttg tattgggctg
     2581 ttaacaccgc cggtgggtag tgcgctattt gttggttgtt ctatttcagg tgtgaagatt
     2641 caacatttga tcaaaccatt gctgccattt tatgccgcat tgttaatcgc gttgatgatg
     2701 atcacttaca tcccgcaaat atccttgttc attccacagc tactagggct aatgtaaaaa
     2761 ataatttgcc ggataacttt ttatattatc cggcaaaggc acttaacatg agttacttaa
     2821 tcaccccatt gcgccgcagt tgttcggtat cttttttcca gcgttcatta gcctggttga
     2881 tatcgtcaat tttcatcaaa atcgcgactt ttttactgtt tatttctgaa actttcatcg
     2941 ttaacggata ttgccctttt tcgagcgtta tccagtcatc ctgcaattta cggctaagtt
     3001 tttgtgattg cagaaaagtc tgtcgccgtt gttcgttgat atcgaaatct cgtttctgct
     3061 gaatcggcac ttcataacac tcttcactct gtttatgaca tgccgcaatc gccggttgat
     3121 aaacctcttt gtcatattta accgcgaacg cctcgctatc gttctgttta ttaaactcat
     3181 cacgctgttg gtgaattttt ttaaaggcgt cttcgcgggt catttgctta ccatttgcat
     3241 cttttcccca atatgcatcc gctgctttct gcgccgccac catttgctta tcaagcgctt
     3301 tcacttcctt ttccagcgca ggaatcgagt tcttctgggt attcaacgca gtctcaagct
     3361 gactaaagcg ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga
     3421 tatcgtcgac cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagtttaccg
     3481 accagccaga cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt atcggtttat
     3541 ctttcgtcca ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga
     3601 gatcgtcact ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat
     3661 caagattttt taaggtgatg gcatcaagct tgagaatgcc gtgatactgc tttttgaatt
     3721 gatcttttaa gatattttca gttggcgatt cgccatctgc ccagacatta ccagtcatca
     3781 acataagcag cgccgtgcca acaatccatt gctttttcac ccgaaaaccc tcgcgcggaa
     3841 taatttattc cggtgattat catcagggta atgaaaattg gcaatggacg taagtctgaa
     3901 gtgaaaaagc cccggcacga taccggggcg aggtgattag tactgattga agatctgctg
     3961 gatctgctgc ggatctttag tttgcgtcag agccagttgc agcagaacgc gcgctttttg
     4021 cgggttcaac gtgccagagg cgacgaagcc gtatttcgta tcatccactt cagcatcctg
     4081 agtggtagcg cccgtcggta cgcgggaaga acgcacgact gcagtgccgt ttttcgcggc
     4141 ggtcgccaga gtgtcaaata cggttttata caggttgccg ttacccacac cagcgctaac
     4201 aatgccatca tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta
     4261 gttataaaca atgccgactt tcggcagttc attcagttta gagacatcga acggcgtgtc
     4321 gctggtgtgc ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtaacc
     4381 cagaggaccg tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac
     4441 atcgcgaccg tccagtacgg tgtcgctcat caccaccagc acgccacgat tagcggaggc
     4501 tttatcagct gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga
     4561 cgtggacggg cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc
     4621 gaggaagtaa gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt
     4681 tttatcgcag tcggtgttaa tttttttcgc cagtgtcagc cagacatcat cgttcatgtc
     4741 ctgggagcca atattcacta cctgctcgcc tttaacgttc gcaatgtcct tcagttgcgg
     4801 caccgcatta accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc
     4861 ggagtcacca ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc
     4921 caatgctgca ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc
     4981 catttcattc ctccagttac gtgaacgcta cgcattatcc cttagctttg tatgggaaat
     5041 ttgacgttaa acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg
     5101 taacaaaacg aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt
     5161 attttaaatt cccgaccagg gctttgcgac tatcttccag agtcacaacg cggctacaaa
     5221 catctttgcc aaactgttgg aaatcttttt cctgcttttt ccattcgctt tggattgagg
     5281 attgcagacc gcccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca
     5341 ccgctttcgc gcccatttca ttaatgctgt cctgtaaaat tccgcccatt gcctgattca
     5401 ctaattgctg gccttcggcg cgaacctgat caatggcttt atagtggaac gtcaggccat
     5461 cgctgcgcgt ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca
     5521 gacggctgcg cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa
     5581 tacgggcttt ctcgacgcgg cttttcgcgc cttcatcaat ccacggcagg gtgctacgga
     5641 gttcagcctg ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac
     5701 cgttatacat cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct
     5761 gcacggtttg cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg
     5821 cgtgagcggt cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag
     5881 tcttccctca agaacaaatc aggccagcat ttgctggccc cagattgata acaaagtgcg
     5941 cgttttccat gccggatgcg gcgtaaacgc cttatccagc ctactcgatc gtgcatcttt
     6001 ataggcctga taagcgtagc acatcaggca gttttgcatt tgtcatcgcc cgtatgcttt
     6061 cttagtccca ccaaacgtcg aaaagttcgc tggtgcgtac ctcgcccagt ttgcgctctt
     6121 ccagccactt acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc
     6181 agatcagacc ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga
     6241 taacctcgtt aataaagtca tcaacggttt tatcaatctg ttcttccgat gtaccttccg
     6301 ggaatcgcca tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac
     6361 gcagacgacg gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac
     6421 tccgtgacca agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg
     6481 cggtacgtaa tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc
     6541 aagcatatgt tccgcataag gttcccagtc ggtcgccata tggaatacgc cccccagttg
     6601 cagtttgctt tttaccagtt cggcaaacgg cacctgaacg atacggcgtt tattatggcg
     6661 cgctttgtgc cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat
     6721 cattttatgc agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaagccctc
     6781 ttcatgcgca gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa
     6841 gtcctgctcg gggcgatctt tagccattgc caccagcgac gcccccatgc cgaaaccaat
     6901 ttcaagcgtc accggcgctt cacggccaaa aagcgcaggg aaatccagca tatcttcgct
     6961 gaactcaacg cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag
     7021 tcgcccctgg cggcgcacaa aactacggat acggcgcagc gggcggccgt tttcatcaaa
     7081 ttccggtgag atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa
     7141 acgggcatta tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa
     7201 gttccggttt acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc
     7261 ggtgaccatg caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg
     7321 gcggaaaacg ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt
     7381 gatgttgcaa caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg
     7441 cttcccaacg gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac
     7501 cgggcttggc tattacgccc gcgcgcgcaa tctgcataaa gcggcacagc aagtggcgac
     7561 cttacacggc ggtaaattcc cggaaacctt tgaggaagtt gcagcactgc cgggcgtcgg
     7621 gcgttccacc gcaggcgcga ctctctcgct ttctctgggt aagcactttc cgattctcga
     7681 cggtaacgtc aaacgcgtgc tggcgcgctg ctatgctgta agcggctggc ctgggaaaaa
     7741 agaagtcgag aataaactgt ggagtttgag cgagcaggtg acgcccgcgg ttggcgtgga
     7801 acggtttaat caggcgatga tggatttggg cgcgatgatt tgtacgcgct ctaagccgaa
     7861 atgttcgctc tgtccgctac aaaacgggtg tattgccgcc gccaacaata gctggtcgct
     7921 ttatccgggc aaaaaaccaa agcagacgct accggagcgc acgggttact ttttactgtt
     7981 acagcacgaa gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcagttt
     8041 atactgtttc ccgcagtttg ccgatgaaga aagtttgcga ctatggttga cgcaacggca
     8101 gattgctgcc gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca
     8161 cttagatatt gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg
     8221 caatgcgctc tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga
     8281 gcgtttgtta cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg
     8341 atgatttatg agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga
     8401 ttttcagctg taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc
     8461 ctgggcgcag tggcagcaca aacaaaccat gctgattaat gaaaagaaac tcaacatgat
     8521 gaatgccgag caccgcaagc tgctggagca ggagatggtc aacttcctgt tcgagggtaa
     8581 agaggtgcat atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac
     8641 gcctccggca acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc
     8701 tcgcgctggc tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata
     8761 cttataacga agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg
     8821 cccacaatat tgagaacatc tggggcttca aagaggtggt gatcgccggt cctaaggact
     8881 acgtgaaata caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga
     8941 tcactatcga aaccatcgcc gggacagaac ctgccgcgca tctgcgtcgg gcaattatca
     9001 aaacgttgct gatgggcgac gatccgagtt cggtcgatct ctattccgac gttgatgata
     9061 ttacgatttc gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga
     9121 ttcgctggga aggtcgcgcg agcaacttcg cggattatct gctgaaaaac cgtctaaaaa
     9181 gccgtagcaa cgggctacga atcatctata gcatcaccat taacatggtg ccgaaccacc
     9241 ttgataaacg tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggca
     9301 ttgatgagtc gctgattctg gcaattatgc agactgaatc ttcctttaac ccgtatgcgg
     9361 tcagccgttc cgatgcgctg gggttaatgc aggtggtaca acatactgcc gggaaagatg
     9421 tgttccgttc gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca
     9481 gcaatattga taccggcacc gcgtatctgg cgatgctgaa caacgtttat ctcggcggaa
     9541 ttgataaccc aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca
     9601 gcgtgctgcg agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga
     9661 cgccaggcga tgtttatcag acgctgacga cccgccatcc ttctgcggaa tctcgccgtt
     9721 atctttataa agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cactaaccgc
     9781 ccctgacgat gctcagggga aaaaatgtta tccacatcac aatttcgttt tgcaaattgg
     9841 gaatgtttgc aattatttgc cacaggtaac aaaaaaacag tccgcgaagt tgatagaatc
     9901 ccatcatctc gcacggtcaa atgtgctttt tcaaacactc atccgcatca cgatgtgagg
     9961 aaattaacat gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt
    10021 ggggaagttg gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg
    10081 cttctattgg cgcagtttat agttcactgg gtatcgccgc ggtctttatg cctgcgctgc
    10141 tggggattgt ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca
    10201 ttggcgctat cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg
    10261 tgatattgat taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt
    10321 actatcgcct gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct
    10381 ggggcaccat cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa
    10441 gccacatgca gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga
    10501 ctctgccgca tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg
    10561 gcctcgatgc attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa
    10621 tgctgctggg cgcggaactg cagattacca acatgttcgg taacaccttc ctgcacagct
    10681 tcgacaaaga tccgatgttt gccagcagct ttattgtgca gcatgcgtca atcatcatgt
    10741 cgatttcgca gatctctgaa accctgttca ttctgactat cccgttcttc ttaagccgct
    10801 acggtattaa gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt
    10861 ttgcttacgg cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt
    10921 acggttgcgc attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta
    10981 gcccggcaat tcgcgccagt gcgcagggga tgttcctgat gatgactaac ggcttcggct
    11041 gtatccttgg cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta
    11101 ccgactggca gaccgtatgg ttgattttcg ctggttactc cgtggttctg gccttcgcgt
    11161 tcatggcgat gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggtgagcc
    11221 actaattacg caaagaaaaa cgggtcgcca gaaggtgacc cgtttttttc ttacttcaac
    11281 acataaccgt acaatcgttt catgccatct gcatcggttt cgctatagac accttgcagc
    11341 tccggcgaaa atcccggcaa caaattcacc ccttcttcca gcgcaaggaa ataacgttga
    11401 accgccccac cccagacttc tccgggtacc acgcaaagca cgcctggtgg ataaggcaac
    11461 gccccttctg ccgcaattcg cccttcggca tcacgaatcc gcaccaactc cacttcaccg
    11521 cgaatataag cgctatgcgc gtcctggggg ttcatcacca ctgacgggaa actctgctgg
    11581 cggaacatcg ctttttgtag gtctttgacg tcgaaactga catacagatc gtgcatctcc
    11641 tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg gatatttgtt ataaacgctc
    11701 ggcaacacct cagccagcgg cgagtcatcc tcaatatgct gttcaaactg cgccagcatc
    11761 gccaccagtt gcgccagctt ctcgtggctt tccgccggag ttaataagaa cagaatggaa
    11821 ttgagatcgc acttctccgg cacaataccg ttctcacgca gatagtgcgc cagaatcgtc
    11881 gccggaacgc caaagtcgct atattcgcca gtttcggcat tgatacctgg tgtagtgagt
    11941 aacagcttgc acggatcaac aaaatactga tccgcggcat atccttcaaa gccgtgccac
    12001 ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg ctaacactga cgtcggataa
    12061 tcctgccaca atttgccatc aacaacgggc gggataaacg ggcggaacag cttacagtgc
    12121 gcaagaatag ccttgcgcgc ttcaatccct atctcaacac actcagccca cagccgacgc
    12181 ccactctctc cttcatgaat tttggcgtta acgtccagcg cggcaaacag cggatagaac
    12241 gggctggtag aagcatggag cataaaggca ttattcaacc gcttatgcgg gcaaaaacgc
    12301 gcctgtccgc ggatatggtt atctttttta tggatctgcg acgtctgtga gaatcccgcc
    12361 tgctgtttgt gcactgactg ggtcacaaag atccccggat cgttttcgtt aagttctaac
    12421 agcagcggcg agctatccgc catcatcggg ataaactgtt cgtaaccgac ccacgcggaa
    12481 tcaaacagaa tgtaatcaca cagatgccca acggtatcga tcacctggcg ggcgttatag
    12541 acagtgccgt cataggttcc cagctgaata atcgctaggc gaaacgggcg cggcaactcc
    12601 gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat actcttcatt aaaacaatgc
    12661 gcatcaatac cgccaatgaa accaaacggg ttacgtgaag cttccagata gaccggcgtc
    12721 gcccccgcct gaatcagcgc gccgtgatga ttcgacttat ggttgttacg gtcaaagagc
    12781 accagatcgc cacgcgttaa cagcgcattc gtcaccactt tattcgctgc cgatgtgccg
    12841 ttcagtacaa aataggtttt atcggcatga aagactttgg ctgcgaattt ctgcgcatcc
    12901 ttcgccgatc cttcatgaat aagcagatcg cccaatttta cgtcagcgtt acacatatcg
    12961 gcgcgaaaga cgttctcacc aaagaaatcg taaaaatggc gtccggcagg atgcttttta
    13021 aagaacgcac catgttgatg cccagggcaa gcaaaagtgc tgttgcccat ctcaacgtac
    13081 tgcgtcagcg tgtcataaaa cggtggcagc aaattctctt catactgaca ggctgcggat
    13141 tccagctcca gccactgctg ctcgttgccg ttgattaccg ccgtaacgcc cgcaggtaat
    13201 tcaacaacat gttcggaata caaaaacacc ggtagatgaa aaccggtgcg cttaagcaac
    13261 gtaagaatgc cactgcgact atccgcagcg gtaatgacga ctgccgcgac gtccgtaaaa
    13321 tcagtatctc ccaacgccac cacgcggcga tgagtagaaa gtcgggatac cagttcacta
    13381 ctggcggcaa tattcattga tttcataagc gcaaacccgt ttcggggaag taagaatacc
    13441 ggacaaggtg aaaaaccctg ccccacgaga tatgggtcaa actggtcacc agctccgacc
    13501 gccagacatc agtaaaagca gaaacgctct gattttactg ttgtcctgca gtgagcgtgc
    13561 gttaacttca ccgcatgagc agaaaagggg aggagtttcg cgcgaaacgg cgataagcga
    13621 gagaatgtaa ggaggtggcg tgcatcgggc aaactccgta cagagaggag aaaattcgcg
    13681 caatcatggc acctttcacc agagcgtgca agccaggact ttgcgaacaa acaagccatc
    13741 gaaggagcca aacggtcata ataagaaaat cagacaatgc agctaacagg aactcttgtg
    13801 gtcatcggtc cttttatcaa cgcaagtgca gtattactgg gtggcgttct cggtgcactc
    13861 cttagccaac gcttaccgga acgtatccgc gtctccatga catcaatttt tggtctggca
    13921 tcgctgggga ttggtatttt actggtggtg aaatgcgcca accttccggc gatggtttta
    13981 gcaaccttac tcggtgcatt aatcggcgaa atttgtctgc tggaaaaagg cgtcaatacc
    14041 gcggtcacca aagcacaaaa tctgtttcgc cactcacgta agaagccagc gcatgaatct
    14101 tttattcaga attatgtcgc gattattgtc ctgttttgcg ccagcggcac cgggatcttc
    14161 ggggcgatga acgaagggat gaccggcgat ccgagtattt taatcgccaa gtcatttctc
    14221 gatttcttta cggcgatgat cttcgcctgc tcgttgggta ttgcggtgtc ggtaattagt
    14281 atcccattac tgatcatcca gttaacgctg gcgtgggctg ccgcgctgat attaccgctg
    14341 accacaccgt cgatgatggc agacttcagc gctgtaggcg gtttattgct gctggcaacc
    14401 ggattacgca tctgcggcat taaaatgttc ccggtggtca acatgcttcc agcactcctt
    14461 ctggcaatgc cgctttccgc cgcctggacc gcctggtttg cctgacaatg cgtgcattag
    14521 cggcaaagtg atgatagatt gtgcagtctg cagtaaattg aagaaatttg attgacgaga
    14581 cgaggcgaat caggtttaat gcgccccgtt gcccggatag ctcagtcggt agagcagggg
    14641 attgaaaatc cccgtgtcct tggttcgatt ccgagtccgg gcaccaaatt catatcaacg
    14701 gacctccacg gaggtccgtt tttcgtttca gaacgccaca atttaagcgt tctgcctcca
    14761 aatcaattct accgaactca accagattcc ccccacatca accccattgt gtgggtataa
    14821 ttgcgggtat accccagttc gacagaattt gtaccctctt taacgcattc agaaggctga
    14881 actatggcac tgactgacgc aaaaatccgg gctgcaaagc acactgacaa ggcttataaa
    14941 ctcactgacg gggctggcat gttcctgctg gttcatccta atggttcccg ttactggcgt
    15001 ctccgttatc gtattctggg taaggagaag actctggcac ttggtgtata tccagaagtt
    15061 tctctctccg aagctcgtac aaaacgggat gaggcccgaa aactgatttc agaggggatt
    15121 gacccttgcg aacaaaaaag agctaaaaaa gtagtccctg atttacaact ctcttttgaa
    15181 catattgcac gacgctggca tgccagtaat aaacaatggg cacaatcaca cagcgataaa
    15241 gtaatcaaaa gcctcgagac acacgttttc ccctttatcg gcaaccggga tatcacaaca
    15301 ctcaataccc cggacctgct tatccctgtt cgtgctgcag aagcaaaaca aatttatgaa
    15361 atcgccagtc gtctgcagca aagaatatct gctgtaatgc gttatgccgt acagtctggc
    15421 atcatcagat ataacccggc tctggatatg gctggtgcat tgaccacggt aaaacgccag
    15481 catcgccctg ctctggatct ttcacgcctg cctgaacttc tgtcgcgtat tgacggttat
    15541 aaaggtcagc ctgtcacccg actggcagtt atgctgaatt tactggtttt tattcgttcc
    15601 agtgaactca gatattcccg ctggtctgaa attgatattg acaatgccat gtggactatt
    15661 ccagccgaac gcgaacctct gccaggcgta aaatactcac accggggctc caagatgcga
    15721 acaccacatc ttgtgccact cagcaaacag gctgtagcca tactgacaga actacagaca
    15781 tgggctggtg aaaatggtct gatctttacg ggagcacatg acccgcgtaa accgatcagt
    15841 gaaaatactg taaataaagc cctgagggtg atgggatatg acacaaccca ggaagtctgt
    15901 ggtcatggat tccgggcgat ggcgtgcagt gcattgattg aatcaggttt gtggtcccgc
    15961 gatgctgtgg aacgtcagat gagccatcag gagcgtaatg gtgtacgtgc tgcatatatc
    16021 cataaagcag aacatctgga agaacgtcga ctgatgctac agtggtgggc agattttctg
    16081 ggtgcgaaca gagaacaatg tatcagcccg tttgaatatg caaagattaa caatccatta
    16141 aaatagtaat catcccgggc tgccagcccg ggaattattt tatgaatatt tttctttgtg
    16201 agtaactggg aaaaggatag cgaagtaaat tactttaatg aattattcca ggggctcatt
    16261 aaagtccttt cgtaatactt tatagtcaaa cacgttatgg ctggcatcaa aaactacgga
    16321 tttcccctgt atcaacgcca cgatttttga ccgttagggg atgcggacga tttcctggac
    16381 tctcaggaga tgaaacatgt ttactgaaga acaaaaaatt cgcaccattg agctttattt
    16441 caaatatggt aagaagctgg ccccgtcgta cgcgaactgg gctacccttc gaaaagaaat
    16501 ctgcggcgtt ggatccgttc atgggaagca ggtggcggat caaaagaatc cattcgtcat
    16561 aagcaccgtt actccgatga gcaaaaacag gttgccgttg aacattatct taaccacggc
    16621 tgctgcctgg cgtttaccag caggactctg ggttatccct gtaccgatat gctcgctcgc
    16681 tgggttaatg aacgttcccc cgacagacga cgtgtattta ccagtaagac caatccggtt
    16741 gcgtcctttg aaccagaagt caaacgtcag gctgtcatgg tactctgtac acgacaagga
    16801 tcagccagtg aaatcgccag gcgtattggt gtcagtcgcg cggtattgta taaatggaaa
    16861 gatgaaatta tcggcaacag tgcttaccag actatgcgta aacataacgc cccttccctg
    16921 gaggcagaac gcgatgcgtt gcgggaggaa gtcgcccgac tgaatcagga aatacgccgc
    16981 cggcagatgg aactggatat tctgaaaaaa gcggaggaaa taataaaaaa agacccgggc
    17041 atcagcatca gtcacctgaa caacagagaa aaaacaaaga ttactgatgc cctgagataa
    17101 acatatcccc tgacagaatt actgcatgtt ctgggccttg cccgtagcag ttatttttat
    17161 caccgggctg cactgaaagc cagtgataaa tacgccacga tacgtacgat gctgacagat
    17221 atatttaaca gtaattacca gtgttatggc tatcgtcgcc tgcatgcgat gctcaggcat
    17281 gagggggatc ggctatcaga aaaggttgta cgcagactta tggtggagga acagcttgtc
    17341 gtcagccgta accgtcatcg ccgctacagc tcatattgcg gagaaatcgg accttctccg
    17401 gataacctta tcgccagaga ttttaaggcg gagcaaccta atcagaaatg gctgacagat
    17461 atcacggagt tccagcttcc tgcaggaaaa gtctggctat caccggtggt ggactgcttc
    17521 gacagaaaag ttgtgagctg gtctctcagt acacgccccg atgctgaact ggtcaacact
    17581 atgctggata acgctgtcga aacgttaaat gctggcgaac gaccggtgat acacagtgac
    17641 agaggtgggc attatcgctg gcctggctgg aaagagtgaa tacagcaggc cttattcgct
    17701 caatgtcccg taaaggatgc tcgcctgata atgctgcatg cgaaggcttt ttcggcagac
    17761 tgaaaactga aatatattat gggcgtaaat gattgggtat cacgcctgaa aaattcatgc
    17821 ggcatgtgga tgcttacatc agatagtata acgagcggcg tataaaattt tcgctgggtg
    17881 cagtcatccc tgaaatgtac cgccaacaat gcgggctggc ataataaagc agtccaggaa
    17941 atcgtccgaa tcctaattac tgtcttatat cattggcgct gatacattaa caggacgctt
    18001 cacctgcgcg taataaacgc tacggtgaac accgaataaa tgacaaataa tccgcactgg
    18061 ccgctttgct ttcagcagtg tgattagcgc gacagcttgc cggggatttc gctcatcagc
    18121 acggcagcct gctttagtat ttctttttcc atctcaaggc gctttatctg cgcttttaag
    18181 ctgctgaatt tctcgctgtt caggggtatg gcattaccag cgggctcaat accctgaagt
    18241 tccagcttat acaaccgtat ccatttacgc aaatggtcag gattgagctc aagtgccagc
    18301 gcgacttctc tgacatcgcg ctggtattta actacttgct cgatagcttc gagcctgaac
    18361 tccggggaaa aggtgcgttt agtccgacga gttttgctca tgcatcaccc cattttcact
    18421 gttttaacat taacaggatt tcgaggtgtc ctgaattacc gatccactac attaattctt
    18481 actggttaat gattaacaat atgaatcatt acatacgcct gttgtatata ttttagcctc
    18541 tcttttacca aacataagaa aactaaacag gtggcaccgc ccctgctggc ccgacagaga
    18601 aaatatctcc ttatcactgc tgcatgagtg gtacaaatca gaaaaaaaac tctcagtgat
    18661 acgactggct gaactccgtg taaaaatgca ttgtgtacga tttacgaaaa acacactgcc
    18721 acacatctgg aatttacaat gtccggagag tgtggagaaa cgaataaaga cagttacggg
    18781 gcaagagttg catacagtcg cctcttctgt aaaactccac acactggaac agagaaggtt
    18841 ctggtaaaca tccatcccgg atacacatat ccaccatgct atggaaatca ggatattacc
    18901 gtgcactgaa taaaataata ttgcatgaat attcagggcc gtcaggattc gttcaacaaa
    18961 gcacaggggc agatagatta tctatactga tttaacagta taaataattc tttcaccatc
    19021 ttgtcagcga tgaacattta tgttctttta tttgaaggaa tctgctctta ctctctgccg
    19081 ggccgcctca ctcatacaga tatcaggcag tcatagattg ttgtgaaaag tagatattta
    19141 ccggatgact gatgcacttt tatataaagg cagaatgttt cagtatatgg aaaatgatta
    19201 aatggaaaac atacactcat tagagaagat aatcaaatat caacaaaaca aagatcccct
    19261 gtatccctgt caggaacatc ttttgataca actctgtatc cgtgcaaaca agaaaataca
    19321 ggataatata tcgagaatgc tgggaggata cggaataaat tactcaacat atatggttct
    19381 caccgtatta tctacagcag ataatcattg tctttcgcct tcagagatca gcagagaact
    19441 ccagtttacc agaacaaaca ttacccgcat tacagatttt ttagaaaaag ccggatatac
    19501 agaaagaact gatagcaaag aggaccgccg tgctaaaaaa atcagtctga catctgaagg
    19561 cctgtccttt attcagggca ttacccttac acaaaaccag tacctgaaag aaatctggag
    19621 tcctctgacc tatgatgaac gtgaactgtt taaagacatt aatcaaaaat tactgacgca
    19681 ttttgctgat gtacgttcat aaagtatgaa aag
//
LOCUS       DARNVM010000086        19599 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.086, whole genome shotgun sequence.
ACCESSION   DARNVM010000086 DARNVM010000000
VERSION     DARNVM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.086"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(115..861)
                     /gene="cutC"
                     /locus_tag="SI621_002768"
     CDS             complement(115..861)
                     /gene="cutC"
                     /locus_tag="SI621_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEI4065459.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(875..1441)
                     /locus_tag="SI621_002769"
     CDS             complement(875..1441)
                     /locus_tag="SI621_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEI4065460.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEKIVASEQSA"
     gene            1657..3390
                     /gene="argS"
                     /locus_tag="SI621_002770"
     CDS             1657..3390
                     /gene="argS"
                     /locus_tag="SI621_002770"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEI4065461.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3443..3835)
                     /gene="flhe"
                     /locus_tag="SI621_002771"
     CDS             complement(3443..3835)
                     /gene="flhe"
                     /locus_tag="SI621_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HEI4065462.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3835..5913)
                     /gene="flhA"
                     /locus_tag="SI621_002772"
     CDS             complement(3835..5913)
                     /gene="flhA"
                     /locus_tag="SI621_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEI4065463.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGTGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5906..7054)
                     /gene="flhB"
                     /locus_tag="SI621_002773"
     CDS             complement(5906..7054)
                     /gene="flhB"
                     /locus_tag="SI621_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEI4065464.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPQVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7256..7900)
                     /gene="cheZ"
                     /locus_tag="SI621_002774"
     CDS             complement(7256..7900)
                     /gene="cheZ"
                     /locus_tag="SI621_002774"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEI4065465.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7911..8300)
                     /gene="cheY"
                     /locus_tag="SI621_002775"
     CDS             complement(7911..8300)
                     /gene="cheY"
                     /locus_tag="SI621_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEI4065466.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8315..9364)
                     /gene="cheB"
                     /locus_tag="SI621_002776"
     CDS             complement(8315..9364)
                     /gene="cheB"
                     /locus_tag="SI621_002776"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HEI4065467.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9367..10227)
                     /gene="cheR"
                     /locus_tag="SI621_002777"
     CDS             complement(9367..10227)
                     /gene="cheR"
                     /locus_tag="SI621_002777"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEI4065468.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHQGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10246..11847)
                     /gene="tap"
                     /locus_tag="SI621_002778"
     CDS             complement(10246..11847)
                     /gene="tap"
                     /locus_tag="SI621_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HEI4065469.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYHISALVFISMIIVAALYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAAATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLHIAPVVS"
     gene            complement(11893..13554)
                     /gene="tar"
                     /locus_tag="SI621_002779"
     CDS             complement(11893..13554)
                     /gene="tar"
                     /locus_tag="SI621_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HEI4065470.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKMYRDIITDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIASGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13699..14202)
                     /gene="cheW"
                     /locus_tag="SI621_002780"
     CDS             complement(13699..14202)
                     /gene="cheW"
                     /locus_tag="SI621_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEI4065471.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14223..16187)
                     /gene="cheA"
                     /locus_tag="SI621_002781"
     CDS             complement(14223..16187)
                     /gene="cheA"
                     /locus_tag="SI621_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEI4065472.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRNQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGTDSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPCEADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16192..17118)
                     /gene="motB"
                     /locus_tag="SI621_002782"
     CDS             complement(16192..17118)
                     /gene="motB"
                     /locus_tag="SI621_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEI4065473.1"
                     /translation="MKNQAHPIIVVKRRKAKGHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEEPEVAPQVSVPTMPSAEPR"
     gene            complement(17115..18002)
                     /gene="motA"
                     /locus_tag="SI621_002783"
     CDS             complement(17115..18002)
                     /gene="motA"
                     /locus_tag="SI621_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEI4065474.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18129..18707)
                     /gene="flhC"
                     /locus_tag="SI621_002784"
     CDS             complement(18129..18707)
                     /gene="flhC"
                     /locus_tag="SI621_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEI4065475.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEDGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18710..19060)
                     /gene="flhD"
                     /locus_tag="SI621_002785"
     CDS             complement(18710..19060)
                     /gene="flhD"
                     /locus_tag="SI621_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEI4065476.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4666 a         5443 c         4808 g         4682 t
ORIGIN      
        1 gcggtaatcg ttcatttttt atactattca gggataaaaa aaggcccctg ttgaaattgc
       61 aggggcctgg tacgagcaag catcatattg ggcgacatga tgcaacggta aaaatcattt
      121 ggcctgatgg cgttcaatga ttcctttcat ttcagcaacc gccgccccgt ctacgacata
      181 acgcgaatac tcgtccgcgt gtgcatctga tgacatggac aatccttgat tacgataacg
      241 cattggtgag gcttgccacg ctcccgcgga gctatggact tccagcactc cggcatcgag
      301 gaagtggtgc aggttttctg cacggactcc tgctccggcc ataatgattg gagcatcacc
      361 atgggcaata agttccataa tttttgataa accttgcagt gcgtctgatt tttgccctga
      421 tgtcagtacc cgagcaacgc ccaattccgc aagattattg agtgtattta aagggttagc
      481 gcacatatcg aaggcgcgat gaaaagtcac tgccagcgga ccggcagcag ccattatttt
      541 ttccattcgt ggcatatcga cattcccgtc aacatcgaga acgcccgtca ccagtccagg
      601 aaaacctaat tcgcggactg tgcgcacatc ctcaagaatg gcggcaaatt caccgtcgct
      661 gtagcaaaaa tcaccaccgc gtgggcggat aatcggatgc acagggatcg tcacccgctg
      721 gcgtacggat ttcagtaccc ccaacgacgg cgttaagccc ccctcttttg gggctgcgca
      781 taattcaact ctgtctgcgc cgttttgctg cgccgttagt gcacattcca tgctgtaaca
      841 gcaaatttcc agtaatgcca tttttactcc ttaattacgc cgactgctcg ctggcaacga
      901 tcttttcaat ggaccacgga tgaaacttaa tagttgtttt gccatcggtg accgccagcg
      961 tcggattcgg taatcgctca tgctcacctt ttggcgaact ggttttcact gagatccccg
     1021 gcaggctgag tccttcatca gaaagcaatg ccagcgcacg ggcattcagc gtttctggat
     1081 cgcccggcag aacgatttca atatgttccc aaccttcgtg cgggtaacgt ttttccccgg
     1141 gccacggtag ctccacaatg gaaaactgcc agtgcgcaac ctgtaccggt tcatgcaatt
     1201 taaacagaca aatcggcctg ccattgatca tattttctga caaaagctcg ccgcactgtt
     1261 caaacccgcg acgccagcgc tcagcggtgg cgttttgatg gcaacgcaaa gaaatgtgat
     1321 cggcagtcag cggagtgata tccaacccca aacggcggga aagttcatct aatgcgtggg
     1381 taaatcgcgg taaatccgat gcaatatcct gcagctcgtc gatagattgc cagttcgcca
     1441 taatcactct tcgtctttca gtaaaagcgt taatttaccc tgttgccctg cgccaaccaa
     1501 ccgctgattt cacgccgctt ctgatgcaat agtgaaaacg gcaatacgcc acgcgcacgt
     1561 tgctgacgaa aacagccatt tgcagtatac tcccgcccta atttctttaa ctggtgcggg
     1621 caatttttgc tcgcttcatc aatgtaaggt attccggtga atattcaggc tcttctctca
     1681 gaaaaagtcc gtcaggccat gattgcggca ggcgcgcctg cggattgcga accgcaggtt
     1741 cgtcagtcag caaaagttca gttcggcgac tatcaggcta acggcatgat ggcagttgct
     1801 aaaaaactgg gtatggcacc gcgacaatta gcagagcagg tgctgactca tctggatctt
     1861 aacggtatcg ccagcaaagt tgagatcgcc ggtccaggct ttatcaacat ttttcttgat
     1921 ccagccttcc tggctgatca tgttcagcag gcgctggcgt ccgatcgtct cggtgttgct
     1981 acgccagaaa aacagaccat tgtggttgac tactccgctc cgaacgtggc gaaagagatg
     2041 cacgtcggtc acctgcgctc taccattatt ggtgacgcgg cagtgcgtac tctggagttc
     2101 ctcggtcaca aagtgattcg cgcgaaccac gtcggcgact ggggtactca gttcggtatg
     2161 ctgattgcgt ggttggaaaa gcagcagcaa gaaaacgccg gtgaaatgga gctggctgac
     2221 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     2281 cgtgcacgta actacgtagt caaactgcaa agcggtgacg aatatttccg cgagatgtgg
     2341 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctgaacgtg
     2401 acgttgaccc gcgatgacgt catgggtgaa agtctctaca acccaatgct gccgggtatc
     2461 gtggcggacc tgaaagccaa aggtctggca gtagaaagtg aaggcgcgac cgtcgtattc
     2521 cttgatgagt tcaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     2581 ggtggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     2641 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggcg
     2701 tgggctatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     2761 ttcggcatga tgctgggcaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     2821 aaactggcgg atctgctgga tgaagccctg gagcgtgcac gtcgtctggt ggcagaaaag
     2881 aacccggata tgccagccga cgagctggaa aaactggcta acgcagttgg tattggtgcg
     2941 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     3001 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcatacac gcgtgtattg
     3061 tccgtgttcc gtaaagcaga aattgacgaa gagcaactgg ctgcagctcc ggttatcatc
     3121 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     3181 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     3241 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     3301 aacagccgtc tgaaactggc acaactgacg gcgaagacgc tgaaactggg tctggatacg
     3361 ctgggtattg agacagtaga gcgtatgtaa tcgcgttata cggcaaaagc cgatgtcatg
     3421 gcagacatcg gcttttttat cattaacgat aattcacaat cacttcattc cgttgtacct
     3481 ttagcggcgg aattaaccgc ccaccgccag gcacttccca gataaatcgc aacggttcta
     3541 tcgccggtat accggaaaag gccacggtgg ttccactctg cccttctaat tcgacacaac
     3601 gagattgtga acacaagcga acccgcagtc ctgcaggtgt cgggccgata agctgataac
     3661 gccacgccac cagcgtcatc aatcctgcag caggttgtcg agtagaaaga ggcgctgacg
     3721 acatcgactc accacggtga ttcagcgtaa cgcccacact actcgcctgc cacatcccct
     3781 ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggtt ctcatcattt
     3841 accgccaatt gtcgccgtca tgcggatatg tcggttatca gatagttcca gattcgacag
     3901 caccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg
     3961 gttcaccaat aaaactggcg gcgcacccaa catctcctga cgggatagag cttcctgagt
     4021 ttgcgccagt aaacgatccg ccagccctgg ctccagtccg cccccgccct gcaacgcctg
     4081 tagcaacaaa cgttccagcg gtgtatcgag gccaataaca tgaacttcat ctttgccagg
     4141 aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg
     4201 tggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatagtgc gcatatcgcg
     4261 aatcggcact ttttcatcga gcagattttg cagcacttta tgcagtgtgg tgagcgtgac
     4321 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag
     4381 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
     4441 atgcgttgcc accaccgtgc tggcctcaac cacggtgtac ccctgaatct gcgcctgttc
     4501 tttaagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
     4561 accaggtaac gtcccggcag cggtacctgg gttaattgcc agccagcgcc ccggataggc
     4621 atcgccagtg ccaatctcta cgcctttcat caaaatgcga taacgtgcag gttgcagatc
     4681 catattgtcg cgaatgtgca ccaccggcgg cagaaacccc atctcctgag caaatttctt
     4741 acgaatacta cgaatacggc ccagcaactc gccatcttgt tggaaatcaa ccatcgggat
     4801 cagtcgataa cccacttcca ttcccaagga atcttccagt tgtacatcgt tccacgtcgc
     4861 ttcgacaacg gtattattct ctgccatttt taccggtttt ggttcggcag gcgctttttg
     4921 ttcacgtccg cggatccacc aggccagccc aagcaatccg gcagtgaaca gcaaaaatac
     4981 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat
     5041 aacgctcggg ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
     5101 gacacgcgta acgatgaccc ccgcggcggt agaaatcacc agcgccggga tttgtgccac
     5161 cagaccgtcg ccaatggtca atagcgtata actttccgcg gcgtgtccca tgctcatgcc
     5221 atgttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc
     5281 ggcgatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc
     5341 ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccggcgtt
     5401 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcccc
     5461 cacttctgcg atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat
     5521 gacgaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc
     5581 gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tgcgggttga
     5641 agctacgtta agtgccagac gcaacaacgt agtaaacaat agaatggtcg gaaacgcagc
     5701 aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat
     5761 attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa
     5821 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc
     5881 aggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
     5941 gggcttccgg caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc
     6001 agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg
     6061 ggatttgttg accgatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa
     6121 gcgtagggac gttattttca gcgccaattt cacgaatgcg taatgccacc agccccgcgc
     6181 ccttggcaac gacttttggc gcactcattt tgttttcgtc gtactgcaaa gccactgagt
     6241 agtgagttgg gttattgacg ataacgtccg ctttcggcac atcggccatc atccgacgcc
     6301 gtgcggccgc tcgctgcatc tgacggatcc gccctttaac ttgggggtcg ccttcgcttt
     6361 gtttgaactc atcacgaata tcctgccgcg acattcgcag ctttttcagg tggctgaaga
     6421 tttggaaaaa gacgtcaaat cccaccatcg gaataacacc aagtaccacc agcagcgcgc
     6481 atagccctac cagatccatc gcattaccca tggcggtaat cggagactcg gccattaagc
     6541 gcatcatctg cggccagtga tgccagagaa aaaatcccgt cacgctgcca accaggatgg
     6601 ttttcaaaat tgccttaagc aactccgcgc cagtctgagc cgagaatatc cgtttaatgc
     6661 ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga ttttccgcta aataccagcc
     6721 ctcccagcat gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca
     6781 gcgccagcat ggcttctctg atcagcagaa taatttgccc gaggatcaga ttcgggtcat
     6841 tgataatact gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca
     6901 gcgacacacc gccaaaccag ataacactaa cgcccactaa caaaatcagc agtgaagtca
     6961 gttcacggga acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg
     7021 gggcttctgt tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa
     7081 gcgtaaatga tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg
     7141 gatttagggc ttttactgcc acacatcaag catagtatgc gtttgtcgga tgcggcgtca
     7201 tcgcgttata cgaccgcctg atatgacgta gtcacgccac atcaggcaat acaaatcaaa
     7261 atccaagact atccaacaaa tcgtccacct gatcctgact ggctaccaca ccggctttgc
     7321 tggtatcgac ctgaggtcca ttaagcaaac tctggttttc acgttttgga cgcgactcct
     7381 gttcggggat gttttccaac agcaccatca gcaactgacg ttcgatctcc tgaatgacat
     7441 ccatcatccg cttaatgacc tgtccggtga gatcctgaaa atcctgcgcc atcatgattt
     7501 ccagcagctg cgcgttagta aagctggtat gcgcggggac atctgccaga aattgccgcg
     7561 tatctgttac cagttctcgg gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc
     7621 aacgttgggt taacgctttt gctgatttct ccatttgatc ctgatgcggt tgtgacgcct
     7681 caacactgtt cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca tagtacaaac
     7741 gatcgcgcgc atcggggatg gcttccgccg cttcggcaat ggcctgatcc agccccagct
     7801 cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg atatcgccag
     7861 ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc tcacatgccc
     7921 agtttctcaa agattttgtt gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc
     7981 acatagccac tggcccccgc ttgcgccgca gcaataatgt tctctttctt cgcttctgca
     8041 gtcaccatta acactggcaa tgctgacatc gcaccatccg cacgaatcgt tttcagcaat
     8101 tccaggccat ccatgttggg catgttccag tcggagataa caaatccata accgcctgcc
     8161 tgcaacttat tgagagcgtc gacaccatct tccgcttcct caacattatt gaatcctagc
     8221 tctttcagca ggttacgcac tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat
     8281 ttaagttctt tatccgccat ttcacactcc tgatttaaat acgtatcgcc tgtccggcac
     8341 taatttttgc caacatttgc tggcttacct ggctaagatc gaccacttcg cagacaccac
     8401 ccatattgat ggcctcgcgc ggcatgccga acaccacgca acttgcttcg ttttgcgcaa
     8461 gggtccatgc ccccgcctga cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac
     8521 cggtcaggat caccccaacc gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca
     8581 catctaccga aggccgatga cggttaaccg ccgggccgtc gtgaattttg atttgataat
     8641 ttgcgccact acgcgccagc tccatatgcc gatcgcccgg cgcaatatag gcatgccccg
     8701 gcaaaacacg ttctccgtct tcggcttctt taaccccgat ctggcaaagc ttattaagtc
     8761 tgtcggcaaa agagcgggtg aaaccgggcg gcatatgctg ggtaattaac agtgccgggc
     8821 tggaaagcgg caacggttgc agtacgtgac gaattgcctc agttccaccc gttgaagcac
     8881 caatcgcaat cagtttttca gaactcaaca acggccctgc cttcagcgtt gtcggtgccg
     8941 acaatggctt atgtgctgca aggctcgcct ttgctgctgt acgcaccttt tcagcaatca
     9001 tttcgctata cgccagcatt ccttcgcgaa tacccagttg cggtttggtg acaaaatcta
     9061 tcgcccccag ctccagcgcg cgcagcgtga cttctgaccc tttgccggtc agggaagaaa
     9121 ccatcactac gggcattgga cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca
     9181 tccgcggcat ttcaacatcc agcgtcaata catcgggatt gaatttctta atcaaatcac
     9241 gcgcaaccag cggatcaggg gcggtcgcca ccatttccat gtcgctatgg ctgttgatga
     9301 tttctgtcat gatctggcgc atcagtgccg aatcatcgac agataacacc ctgattttgc
     9361 tcatcgttaa tccttactta gcgcatacac cgtctgacca cgcagcgtga agcggcgctc
     9421 aaggtggcta aagttttcag agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg
     9481 aacaaagcgg cgcaaaatct cctgctgggt agtttgatcg aagtagatca tgacgttacg
     9541 acagaagatc gcatcaaacg gccccggcac ggtgtactgt ttcgccagta aattcagcgg
     9601 ggcaaaatca acataattcg ccaattcctg acgcacgcgt accagccctt gatgcggtcc
     9661 cgtccctcgc atgaagtacc gctgcagttg ctgcggcgtc agatttttca actcttcatg
     9721 gcgataaata ccgcttctgg ctttttccag cacttcggtg tcaatgtcgc tggcaaacac
     9781 tttccagcgt ccgggcgcgg tgcccaatgt gtcagccagt gtcatcgcaa tgctgtacgg
     9841 ctcttcgccg gtcgaagccg ccgcgctcca tacgcgatac tcgccagaac gacgccgggc
     9901 gtgatccgcg agcagaggaa aatgatgcgc ctcacggaaa aatgccgtca gattcgtggt
     9961 cagcgaattg ataaacgcct gccactcacc gctgtgctga ttagattcca gcaagttcag
    10021 gtaatggccg aaatccgtca gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac
    10081 catgtcgcgt ttatggtcgg ccaggacgat cccggctcgt tgatagatca attgacttat
    10141 ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca ataaagacgt
    10201 ttgcccacag ggcagagatg aagtcatagc gccttctcaa tcacttcagg ataccactgg
    10261 cgcaatatgt aactgcgccg actcatgtcg tgctacttca tgttcttcaa gggtaaatac
    10321 cgccacacgc gacgaaagat ggtcagcctg gttcgccagt tgttccgttg ccgccgccgc
    10381 ttcttctacc agcgaggcgt tctgctgtgt cacctgatcc atctggctga cagcctgcgc
    10441 aacctgctca atcccccgcc gttgttcttc cgaggccgaa gcaatttctc ccataatgtc
    10501 gttcacgcgg gtcaccgaac tgacaatatc agtcatggtc gcggcggcgt tattcaccag
    10561 tttcgaaccc tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc
    10621 tgcctgggcg ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg
    10681 accctgctct ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg
    10741 gaaagcaatg ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat
    10801 ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc
    10861 ggcatttttt gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc
    10921 ggttaattgc tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg
    10981 acttgagaga tcgttattgc ctgcgacaat ctccgcgata ccaatgtgca tctcctggct
    11041 tcccttacgc acatcactta ccgtcccacg caaagcctgc tgcatggtct tcagactggc
    11101 aaaaatggcg gtgatctcat tgcgaccata cactgcaatc ggacgcgcca gattacccgc
    11161 agcaatgctg tcaaaatggc taccgatgat ggccagtggt tgaacaatca tcttgcgcgt
    11221 ccaccacagc gcactgctga tgtagagcgc tgcaacaata atcatgctga taaacaccag
    11281 cgccgaaatg tgatagttac gctggctttg cgcactggca gcttccagca catggttgat
    11341 ctccagctgc caggcctcaa agttaacgtc aaacgcattc tgtgattcct gcaccggcgc
    11401 agtgaggaaa tccgaaagtt gattactttc aagccaggtc gcctgatgtt cgagatcgtt
    11461 gtgccagcgg gcaaaacttt tctccgtctc tttttgcaac gccctgacgt gctcgttgcc
    11521 cgcagtcatc gccataaaac ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc
    11581 cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta agcgccgtca gagtgcccgc
    11641 tttgttcagc gcggtactgg cctgtaacat taccgcccga gtttgcgcta aagccgcacg
    11701 ttgctgattg ctctgttcga cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa
    11761 agacatgccg ttactgccaa tctgcaagat cccgcagaga atcaaaatta aaaacagcgt
    11821 ggtcgaaatt cgaatacgat taaacatcaa cgctccccat caggcggcaa tgaccgcgtt
    11881 agtaaatact cgtcaaaatg tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg
    11941 ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg
    12001 gctggctgcc agacgaaacg cggaaaccgc ttgcgttaaa cgactggcct gttcttccag
    12061 cgcagctgcg gcggcagctg attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc
    12121 catttccgaa accgccaatg cgacttgatc gatgccacgg ctctgttcat ccgatgccga
    12181 tgcaatctcg cccataatgt cagtcacgcg agtgacagca ttgacgatat tgttcattgt
    12241 ttccccggcg ctttcgacca gcaccgaacc ggtatcaacg cgcgagacgg agtcttcaat
    12301 gagggctttg atctcttttg ccgcctgggc gctgcggctg gcaagattac gcacttcgcc
    12361 cgccaccacg gcaaaaccac ggccttgttc acccgctcgt gctgcttcaa cagcggcatt
    12421 cagcgcgagg atattagtct ggaaggcaat accatcgata acgctgataa tgtcggcaat
    12481 tttcttcgaa ctatcggcga tctcatgcat cgttttcact acgccatcca ctactttgcc
    12541 gccgtgctgg gcagtgtcag aggcactttg cgccagttgt gaggcctggc gggcgttatc
    12601 ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg gcggcagttt cttccagcgc
    12661 agatgcctgc tgttcagtac gggaggaaag atcggtgttg cctgccgcaa tttcacgggt
    12721 accggcatag atggcatctg aaccttcacg cacatgagtg acggtgtcag tcaaagagcg
    12781 ttgcatatgt gaaacgctct gcgccaggtc gcccatttca ctgcgcccgt caatggtcag
    12841 ggtattcgcc aggttaccac tggcgatttc gcgaatgtga gcaataattt ttgccagcgg
    12901 tgtaagcaac atacggcgaa tgccgtacca cgccaccagc agaatcaata ccaccaccag
    12961 cgcaataacc gccagttgcc attgggcaaa tcggtaatca tctgcgttgt cagtgatgat
    13021 atcgcgatac attttttcac tgctgagggc gtactgagca aacgcttcgc ccattgcatt
    13081 ttgcattccc tgggttggct gagcgaaata agctccagta ttgccataat caagataatc
    13141 aatcagttca gttaacgctg tgtgatagtt tttatatttt tcatcaatat tacgactggt
    13201 agcgaccatt tcaggtaacg gtgccatgct tttgaatttt ttataatgcg tcgctgcctg
    13261 ggccaatgtt ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt gttgattgga
    13321 ggaatccatc atcatccgca ccgctgaacg actcaggtta atgcgcgttt gcagcattaa
    13381 atcccaggtt gacgtcagct cgccctgctg ttcccgtaat tgattggaaa ccacaaagct
    13441 cttctggcta tggtgaaggg aagaaaaaaa cagactgccg gaaataagct gtaacagtgc
    13501 gaataccccc agcaccatta ccaacagcgt gactacgcgg atacggttaa tcataaggca
    13561 ccttcctgaa aacaagttga tctcgttatc ggcaaggaag ggggaaactt tattgctgat
    13621 gccacccgcc gcgaaactga aataaaaaac ccgatgcgca ggtcatcggg ttcattgcaa
    13681 ttgaggaaat cgggagaatt acgccacttc tgacgccgcg ctatctaaca gcgccatctc
    13741 ttcgctgttc agcagttttt cgatgttcac cagaatcaac atccggtcgc ccagtgcgcc
    13801 cagtccagtg agatattctg ttgaaagcgt cacggcaaat tccggtgccg gacgaatttg
    13861 ctccgccgtc aatgaaagca cgtctgaaac gccgtcaacc acgatgccga ccacccgctg
    13921 tccgagattc aggacgataa ctaccgtgtt gtcgttatag tccacatcca cctggctgaa
    13981 cttaattcgt aagtccacaa tcggcacaat aacaccgcgc agattcgtga cgcctttgat
    14041 aaacgctggc gtgttcgcaa tccgtgttac ctgatcatag ccacggatct cctgcacttt
    14101 caggatatca ataccgtact cttcatcacc aagggtaaat accagaaatt cctggcctga
    14161 cggctcgctg gccagctttg ttacattcgt cataccggtc atattgttac ctttttactc
    14221 attcaggcgg cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa ggcggagaca
    14281 tcaacaatca gtgccacgct gccgtcgcca agaatggtcg cagcagaaat gccggggact
    14341 ttgcgatagt tactttccag gtttttaacc acaacctggt gttgaccaat taattgatcc
    14401 accagcaagg cgtagcggcg accgccactt tgtaagatca ccacaattcc ctgggtggct
    14461 tcggttttcg cgcccgcgac gttgaacact ttccacagtt cgacgatagg cagatattca
    14521 ccccgcactt ccagcacccg ctcgccgcca gccagtggat gcagatcggc ttcacagggt
    14581 tgcagtgatt ccatgacagc attcagcggc agaatgaaaa cttcatccgc aacgcgtacg
    14641 gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta
    14701 ccctgcttcg actggatttc gacatgaccg cccatctcct ggatattacg tttaacgacg
    14761 tccatgccga cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga gaagccaggt
    14821 gcaaatatca gcattgcgac ttcgtcgtcg ctcatgtttt cgctgacggt cagaccttgc
    14881 gaggccgctt ttgccagaat tcgctcacgg tttagccccg cgccatcgtc ggtcacttca
    14941 atgcaaatgt tgccgccctg atgttcggca gaaagaatta aatttccgac gctattttta
    15001 cctgcggcga gccgtttttc tggcagttca ataccgtgat cgaggctatt gcgtaccagg
    15061 tgggtcagcg ggtcgataat gcgttctatc aggctcttgt cgagttcagt ggaactgccc
    15121 accagcgtca gttctacctg cttgccgagt tttcccgcca gatcacgcac cagccgggga
    15181 tagcgactaa agacatattc catcggcatc atgcgaatcg acatcactga ttcctgcaaa
    15241 tcacgagcgt tacgttgtaa ctgccccatg ctggtaatca aatcaccatg attaaccggg
    15301 tccagttcac tggagcgctg ggcaagcatg gactgggtga tgaccagctc gccgacgagg
    15361 ttaattaatt gatcaacctt ttctaccgct acacggatgc tggtggattc actactgcgc
    15421 gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt cggctgccag tttaagcact
    15481 ggtggggggg atattttggg cgagacttct actgtttcaa aggtaatctg atcggcttca
    15541 atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tgtcgcccgg taatattgcc
    15601 gagagagaat ccgtcccttt caccacgtca gttaacgttg tcagatgccc cagctcttct
    15661 tccagcaggt cgacttcccc ggccttcagg cgcgaaagga taattcgtcg cggcaactga
    15721 ttgcggctct gctcatcttg cggttcactt ttggcaacca cacttaatcg ggtcactgcg
    15781 gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca gatataatcg
    15841 aagctggcag catccggctc ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg
    15901 tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc acctcgtctg
    15961 gcttcgtcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct gaagccaaag
    16021 gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa ttgttcggca
    16081 tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tgtcagccaa cagttcgtcc
    16141 gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca tgctcacgct gtcacctcgg
    16201 ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt cttccagggc
    16261 gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt
    16321 gttcagtacc agcaggctga tgcgacggtt gacagcatca tcaggaccgc gatcgcttaa
    16381 gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgccattca acccgccaac
    16441 catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt tgctatatcc
    16501 tttctcacca ctggcgtaag ggaaatcatc ggtatgaccc gaaaggctaa tacggttggg
    16561 aataccattc agaacgggcg cgatggcgcg cagaatgtcg cgcatatagg gttccacatc
    16621 ggcactgccg gttctgaaca tcgggcgatt ctggctatcg atgatctgaa tacgtagacc
    16681 ttcctgaacc agatcgattt tgagatgtgg gcgtaacgcc cgcagtttcg gatcggattc
    16741 aatcaactgg tcgagatcac cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt
    16801 cagctcttcg atgttcggct gcttattcac ttccccctgg ctttgggtgt aatcatcacc
    16861 cccaccggga attgggcttt cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc
    16921 cagtggagtc cggaagtact ccgcaatctg aatcaactct tttgggctgg agatggagat
    16981 cagccacatc accagaaaaa aagccatcat cgcggtcata aagtcggcat aagcaatctt
    17041 ccacgatcca tgtgctgccc cgtggccttt ggctttgcgt cgtttgacga caataatcgg
    17101 atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca
    17161 cgcacatgct cttccagttc aataaacgac ggacgttcgc tggaatagag cgttttgcga
    17221 ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag tgtgactttg
    17281 acgcactgca tcattttgct ggtttcggcg cttttctgac gtaaaacact cgctaatggg
    17341 gaaataaatc cgtaagccaa taaaataccg aggaaagtcc ccaccatcgc atgtgcgata
    17401 agcgccccca gctcggcggc aggacgatcg gctgaaccta acgcgtgaac gacccccatc
    17461 acagcagcga caataccaaa cgccggaagt gagtccccga ccagcgccag actgttcgcc
    17521 gggacttctg cctcgctttc gtgcgtctca atctcttcat ccatcagggc ttcgatttcg
    17581 aaggtgttca tgtgaccgct gataatcaga cgcagataat cgacgataaa atcaagcatg
    17641 acgctatccg caaggatgcg tgggtagctg gcgaagatct cgctctcacg gggattttca
    17701 atatcacgtt ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa ccgataaagc
    17761 agagccagca gatccatata cattgctttg gtgtatttgg agcgacgaaa cagcaacggc
    17821 agcgccttca gcgtgccttt aatcgctttg ccattattgc cgacgataaa cgacccaatc
    17881 cctgcaccgg caataatcac cagttcagcg ggttgataga gtgctccaag gcttccaccg
    17941 gtcatcaaat aaccgccgaa aactgtaccg agaacaacca ggtaacctaa taagataagc
    18001 acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg cgttaacgcg
    18061 cgattggggc aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt tgcgccacac
    18121 cgtataagtt aaacagcctg tactctctgt tcatccagca gttgtgggat aatatcggca
    18181 ggattctggg aaagtttacg tctttttact gcccgggatg gcggctgaca taagctgcag
    18241 gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca gcagttgcag
    18301 ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc acgggttaat
    18361 gccagcagcg gtccgtcttc tgcttgcggg cactgttcaa ggtataaacg gtaggctttg
    18421 atcaccgcat cgacgccatt acacaaaccg gttttcagta aaaactgcca tgcgttacag
    18481 aacatcgaag catgaacgtt ttgttcccag gtcataaacc agtcggttga gaatggcagc
    18541 atgcctttcg gcggtgggct tccgcgcagt tctttataga gttttatcag gcgtccgcga
    18601 cttaactgtg tttcgctttc cagcatctgc aaacgagcgc caagggtgat caattccatt
    18661 gccagctgaa tatcccgcgc ttcctgaaca atgctttttt cactcatgat caggcccttt
    18721 tcttgcgcag cgcttcttca ggctgattaa catcattcag caagcgtgtt gagagcatga
    18781 tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac tgagtaatcg
    18841 tctggtggct gtcaaaacga aagtgacaaa ccagttgatt ggtttctgcc agcttaacca
    18901 tttgcggaag agtcagtgcc gctaacgttg tcgccatttc ttcatttatg ccgagacgaa
    18961 acattgcgga cgctttgtcc tgaacaatca gacgctgtgc aagtagtaaa tatgacaagt
    19021 tgatgtcata aatgtgtttc agcaactcgg aggtatgcat tattcccacc cagaataacc
    19081 aactttattt ttatgcggtc tcaccgcacc ccgtgatgtc gccgggaagc cccggtaaaa
    19141 aataattagc attagaatag ttgcgataag ctgcaatagg cagagcccct ttttgggatt
    19201 taataagtcc ttacaaatag aaggggttct ttacacttat ctaagatttt tcctaaatcg
    19261 acgcaacttt actcgtcact acactcacat acaacggagg ggggctgcga ttttcaataa
    19321 tgtgtgatgc agatcacaca aaacgctcaa ttacttaaca taaatgtata agtcatactt
    19381 ttgttttggg tgtatttcaa tctgttaaaa agtttttcgc gacgctagca agcaaaaatg
    19441 aaacaggaat aatcgaaatg ggatgttgcg cacagttaaa ataactcacc gtaaataatc
    19501 atctgctata aataattgct ttaatgcaat accagataag ctatttttaa acagacactt
    19561 accgcacaac aaactaatta aatacaaaga aaggcaaca
//
LOCUS       DARNVM010000087        19557 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.087, whole genome shotgun sequence.
ACCESSION   DARNVM010000087 DARNVM010000000
VERSION     DARNVM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.087"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..142
                     /gene="xisR"
                     /locus_tag="SI621_002786"
     CDS             <1..142
                     /gene="xisR"
                     /locus_tag="SI621_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HEI4065477.1"
                     /translation="QIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYNYPKINQFIK
                     DS"
     gene            144..1379
                     /locus_tag="SI621_002787"
     CDS             144..1379
                     /locus_tag="SI621_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEI4065478.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            1431..2366
                     /gene="ttcA"
                     /locus_tag="SI621_002788"
     CDS             1431..2366
                     /gene="ttcA"
                     /locus_tag="SI621_002788"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEI4065479.1"
                     /translation="MQENQKNTKKEIYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2495..3868)
                     /gene="dbpA"
                     /locus_tag="SI621_002789"
     CDS             complement(2495..3868)
                     /gene="dbpA"
                     /locus_tag="SI621_002789"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEI4065480.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLETEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGRIKGKT
                     CRVRLLK"
     gene            complement(4346..5329)
                     /gene="zntB"
                     /locus_tag="SI621_002790"
     CDS             complement(4346..5329)
                     /gene="zntB"
                     /locus_tag="SI621_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEI4065481.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5584..6816
                     /gene="dgcM"
                     /locus_tag="SI621_002791"
     CDS             5584..6816
                     /gene="dgcM"
                     /locus_tag="SI621_002791"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HEI4065482.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCTLARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6837..7400)
                     /gene="smrA"
                     /locus_tag="SI621_002792"
     CDS             complement(6837..7400)
                     /gene="smrA"
                     /locus_tag="SI621_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HEI4065483.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7595..9109)
                     /locus_tag="SI621_002793"
     CDS             complement(7595..9109)
                     /locus_tag="SI621_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEI4065484.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFDR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(9106..10134)
                     /locus_tag="SI621_002794"
     CDS             complement(9106..10134)
                     /locus_tag="SI621_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEI4065485.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSTGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            10231..11046
                     /locus_tag="SI621_002795"
     CDS             10231..11046
                     /locus_tag="SI621_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4065486.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(11219..12127)
                     /locus_tag="SI621_002796"
     CDS             complement(11219..12127)
                     /locus_tag="SI621_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4065487.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKHLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            12303..13613
                     /gene="abgA"
                     /locus_tag="SI621_002797"
     CDS             12303..13613
                     /gene="abgA"
                     /locus_tag="SI621_002797"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HEI4065488.1"
                     /translation="MESLNQFINSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEERDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVPGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            13613..15058
                     /gene="abgB"
                     /locus_tag="SI621_002798"
     CDS             13613..15058
                     /gene="abgB"
                     /locus_tag="SI621_002798"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HEI4065489.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQASCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            15095..16621
                     /gene="abgT"
                     /locus_tag="SI621_002799"
     CDS             15095..16621
                     /gene="abgT"
                     /locus_tag="SI621_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HEI4065490.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            16632..17147
                     /gene="ogt"
                     /locus_tag="SI621_002800"
     CDS             16632..17147
                     /gene="ogt"
                     /locus_tag="SI621_002800"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEI4065491.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            17342..18094
                     /gene="fnr"
                     /locus_tag="SI621_002801"
     CDS             17342..18094
                     /gene="fnr"
                     /locus_tag="SI621_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEI4065492.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            18246..19196
                     /gene="uspE"
                     /locus_tag="SI621_002802"
     CDS             18246..19196
                     /gene="uspE"
                     /locus_tag="SI621_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HEI4065493.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(19246..19503)
                     /locus_tag="SI621_002803"
     CDS             complement(19246..19503)
                     /locus_tag="SI621_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HEI4065494.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
BASE COUNT         5199 a         4817 c         4637 g         4904 t
ORIGIN      
        1 acaaattgaa gcataccgcc agaactgttg ggtggaaggc ttccacttca aacgagtatc
       61 tcctttaggg aagccagaca gtaagcgagg aattatctgg tacaactatc caaagataaa
      121 tcagtttatc aaagactcat gatatgtcta aattaccaac aggtgtcgag attcgaggta
      181 aatacattcg catctggttc atgtttcgag gaaaacgatg tcgggaaaca ttgaaaggct
      241 gggaggttac taacagtaac attaaaaaag ccgggaattt aagagcgttg atagttcatg
      301 aaatcaattc cggtgagttt gagtatttaa gacgttttcc ccagtccagc actggggcaa
      361 aaatggtgac aacgagggtc ataaaaacgt tcggggagct ttgtgatatc tggacaaaaa
      421 ttaaagaaac agagttaaca acaaacacaa tgaagaaaac gaaatcacaa ttaaaaacac
      481 tcaggataat aatttgtgag agtaccccga tatcgcatat tcgttatagc gatatcttaa
      541 actaccggaa tgaactgctg catggagaaa cgctttacct agataatcca agatccaaca
      601 aaaaaggaag aaccgtgcgc acagttgata attatatcgc cctgctctgt tcgttgttac
      661 gttttgcgta tcagtcggga tttatatcaa ccaaaccatt tgaaggagta aaaaaattac
      721 agagaaacag aataaagcct gacccgttat ctaaaacaga attcaatgca ttaatggaaa
      781 gtgaaaaagg acagagccag aacttgtgga aatttgccgt atactccggg cttcgtcacg
      841 gggaactggc tgctctggcg tgggaggatg tggatttcga gaagggagtt gtgaatgtca
      901 gaagaaacct gacgatactt gatatgttcg gtcccccaaa aacaaatgcg gggatccgga
      961 cggtaacact actgcagcct gctcttgaag cactgaagga gcaatacaaa ctgaccgggc
     1021 atcatcgcaa aagcgaaatc actttttatc atcgggagta cggcagaacc gaaaagcaaa
     1081 aactgcattt tgttttcatg cccagagtgt gtaacggaaa acagaaacct tattactcgg
     1141 taagcagttt gggtgcgaga tggaatgcag cagtaaaacg tgctggtatt cgccgccgta
     1201 atccgtacca tacgcggcat acttttgcct gctggctgtt gacggcagga gcgaacccgg
     1261 cgtttatagc cagccagatg gggcatgaaa ctgcgcagat ggtgtatgaa atttacggta
     1321 tgtggattga tgacatgaac gacgaacagg tagccatgtt gaatgcgcgg ttatcgtagt
     1381 tgcaaagttt gcccccaatt tgccccattt agtaccagag aactgaaata atgcaagaaa
     1441 atcaaaagaa tacgaagaaa gaaatataca acctgaacaa attacaaaaa cgtctgcgtc
     1501 gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg
     1561 tttgcctctc cgggggtaaa gacagctata ccatgctgga aattctgcgc aatttgcagc
     1621 aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccgggct
     1681 tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac aagattgttg
     1741 aagagaacac ttacggtatc gtgaaagaga agattccgga gggcaaaacc acttgctcac
     1801 tgtgttctcg tcttcgtcgc ggtatccttt atcgtaccgc aacggaactg ggagcgacga
     1861 agatcgcgct gggtcaccat cgtgacgata tcctgcaaac gttgttctta aatatgttct
     1921 acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatatcg
     1981 tgattcgccc gctggcctac tgccgcgaga aagatattca gcgctttgcc gatgcaaaag
     2041 cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtga
     2101 ttgctgacat gttgcgtgac tgggataaac gttatccggg gcgtatcgag acgatgttca
     2161 gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc gatttcaaag
     2221 gcattaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat cgcgaagaga
     2281 tccctctaca accggcaggt tggcagcctg aagaagatga aaatcagttg gatgagttac
     2341 ggctgaatgt ggttgaagtg aaataacccg gatagcgccc gatgcgtaag cgtatcgggc
     2401 tactcttatg gaggccggat aagacgcggt cagcgacaca tccggcaatg ccgaagaaga
     2461 tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc acccggcacg
     2521 tttttccttt aatcctcccg ccctgtaact gtttccatgc tttatgagca acagcctgac
     2581 gcaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct gcgccatcaa
     2641 gcccgatatc tcctgtcagc gcacctaata catcacccgg gcgcattttg gcttttttcc
     2701 cgccatcgat acataacgtt gccatttcgg tttccagcgg cacaatagaa ctattaactg
     2761 gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg gcccgctgtg
     2821 cttcttccgg ggcacagaaa ctgatcgcca gaccgctatt tcctgcacgc gctgtacgac
     2881 cgatgcgatg cacatgaact tcaggatccc acgccagctc aaagttcacc accagctcaa
     2941 gcgatttaat atccagacct cgcgcagcaa catcagtcgc aaccagtaca cgggcgctac
     3001 cgttagcaaa acgtaccagg gtctgatcgc gatcacgttg ctccagatcg ccgtgtaacg
     3061 acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa tcttttttgg
     3121 tattgcaaaa caccacgcaa gaggatggct gatgcaaact taataaccgt tgcaacaacg
     3181 gaattttgcc tttgctggat gtctcataaa attgttgttc aataggtggc agagcatctg
     3241 ttgagtcaat ttcaatcgct aaaggatcgc gttgcactcg tccgctgatt gcggcgatgg
     3301 cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgctggcgca aaacggatga
     3361 catcatcaat ggcgtcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca
     3421 gcgtattcaa cgcatccagt gataccgtgc ctttttgcag atgatccaac aaacggcccg
     3481 gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata ccgaacggtt
     3541 gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccaac cgacgcaatt
     3601 cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa
     3661 atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc
     3721 cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct
     3781 gcaccggcgt catagttaaa taacccaact cattaaggtt cgtgagttgg gcgggaggca
     3841 aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtta
     3901 gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc
     3961 gggtggtggg tcaggcatgg gttgcggacg agggatcgga tcgggcactg gaacaggatc
     4021 gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc
     4081 cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg
     4141 actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag
     4201 taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagagagata
     4261 ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata
     4321 cagacatccg ggaaaattgc ttttgttaca accatttact acgatgcaac cataaagcaa
     4381 caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc
     4441 cgcctgggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta ctgggtaaaa
     4501 agaccattgc catcaacgac attgtatagg tacgacgcgc taaattttcc tgcatcacct
     4561 gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaagcc
     4621 cgcgcccaag gcgatcggca atatcctgca tacgacggcg ttggtcatcg ctcatccacg
     4681 gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca
     4741 tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa
     4801 ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcgctggaat
     4861 gatcggtcaa cgcatcacat acatccacca gccatccccc gcaatcggtc ggacccgtgc
     4921 cctcttccag atcgcttacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa
     4981 caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg
     5041 tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg ctgactcggg
     5101 gccgtgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg
     5161 ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag ggatgcgctt
     5221 catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg
     5281 caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca gtgcctccat
     5341 catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc
     5401 cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt
     5461 ttccatatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat
     5521 catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taacgggatc
     5581 tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt ctggatcgtt
     5641 tcgccctttg aggaacagtt aatttatgcc aacagcgcgg cgcgactgtt gatgcaagac
     5701 ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa agaactgccg
     5761 aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac cgttcagcgc
     5821 aatgaagagg aaacagcatt gagttgtcgg cttgttttgc gaaagctgac agaagcagaa
     5881 ccggtgatta ttttcgaagg tatcgaagca ccggcaacgc tgggtttaaa agccagtcgc
     5941 tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct
     6001 gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgttgatgc taacctcgcc
     6061 gcgctcaatt tctatggcta taaccatgaa acgatgtgcc agaaacatac ctgggaaata
     6121 aatatgctcg ggcgacgcgt catgcctatc atgcatgaaa tttcgcattt acccggtggc
     6181 cataagcctt tgaattttgt tcataaactg gcggatggtt cgactcgtca tgtgcagacc
     6241 tatgccggtc cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt
     6301 actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga cgcgatgacc
     6361 ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat gcagcacctc
     6421 gccatcgctc aggattacag cttattgctc atcgacaccg atcgttttaa acacattaac
     6481 gatctctatg ggcattctaa aggtgatgag gtgttatgca ccctcgcccg cactctcgaa
     6541 agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg
     6601 ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg cgtaagcgtg
     6661 gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt ggcgcatcac
     6721 gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgggcg
     6781 aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc tcgagatcaa
     6841 cgactgcgct tagcgtggcg ctcccagttt tcttgctttg cctgtgccgt tttacgtagt
     6901 gcgacgtaac acgccccgct gccgccatga tgcggtagcg cggtacaata tgcctggaca
     6961 tcatcaaatt cggtcaacca gcgcgccaca tagctgcgga caatattggc atgcgattta
     7021 tcttcccgcc ctttaccatg aataatcagc acattacgca aaccatccgc cagggcttgt
     7081 tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa aagattcagg
     7141 ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg ttgcaaccct
     7201 tcccgccgaa actccagcgg ctgacttagc gggatgatgt cgagaaatcc ggtggtgagg
     7261 aaattatcaa gttgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc
     7321 cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc
     7381 gatttgtcgt caaggttcat gtaacctcct atgcaccttc acacgtcgtg attcagacgg
     7441 gttaattgtt catacttact gcatcgttcc cttcacagat tgaaacaata ctctacttca
     7501 tacctgtgag tatctgcgca ggcggggcat tttatactag cctgttagca gtatcgaaca
     7561 gtttagcccg agtttaattc agcacaggct ccgatcagga tgatttattc atagcaggaa
     7621 ccaacaaaaa tgcgataaat aacagaccac tgattagcag aaaggtatcc gaaaatgcca
     7681 tagtttgcgc ctccctcagc atcaattgac tcaataattt ggttgaagcc agtatttccg
     7741 atgtctggtc acttccactg cgatcaaaaa acaaagcact tctggaaatg aagtcattca
     7801 ccgtatgggg aacagaaacc attttttcac ccattctcga aaaatgaaaa ttagttcgat
     7861 tattgagaat agagccacac agggcaatac caatcgcgcc cccaagatta cgtgttaaat
     7921 taaatacacc agaggctaac ttcagccgtt cttttggaat tcctcccagc gttaatgtta
     7981 cgatgggggc catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga aacagtaact
     8041 cctgccatcc ccattcgtgg gttatagggg taaaaagata cattgaaaac acaaatccac
     8101 ccagccccgc catcaacagc catcgcagat ttattttttt acttaaccag aagtaaaagg
     8161 gaacagaaaa taactggaaa atacctgttg tgcaaacggc aaaacctatc tcttcggcat
     8221 ttaatccgcg aacctgcccc aaaaacacag gtattaaata tacagtagag aatatcccaa
     8281 cccctccaga aaaagaaaaa aaacatccca gcgtgaaatt tttatcctta aaagcatgaa
     8341 gatccattat cggattggat attgtaagtg tacgcgcagc aaataaaata aaactcacca
     8401 atgccagaac tgatgtcagc aaaatagtat tatcatccag ccatccccag cgcgctcctt
     8461 cttcaagagt atattccaga catcccagtg tcatggccaa caggataata ctcggataat
     8521 cagcaacttt cagtaaagag agatcaggtt tatcaaaatt aaccagaaaa ggaatactca
     8581 aaactaaata tatgccaggt aatatattga tataaaatag ccatcgccag tcgaggttat
     8641 cagtgatcca gccccccaga gttggcccta gcgtcggtga cagtgaggcc agcgcactga
     8701 ctaccgcagc agccaacccc aactctttac cctggtaata aataaacgcc gtcgtgaaaa
     8761 ctaaagggat catggatgcg ccagccgctc cctgtaaagc acgaaacaaa atcatgatct
     8821 gaatattcca tgcaagtcca cacgcgatac tcattaaagt aaatattccc gcggatagag
     8881 taaaaagcca acgagtggaa aatacccgtg acagccaccc agagagcgga ataacaataa
     8941 tctcagcaat taaatagctg gtctgaagcc aggccatttc gtctttacct gcggacagac
     9001 caccacctat ttcatttagg gatgacgata caatttgaat atcaagctga gcaataaata
     9061 atcccataca catactggcg aaagcaatat gtttacgcat agacatcatc tctcatcaac
     9121 actgacggtg acggacagtc ccgggcgaag aagttgcttt aattcctttg cgtcgtcaaa
     9181 tgcaatccgt accgggacac gctgaacaat tttggtgaaa ttgccagtgg cattttctat
     9241 cggaatcaag ctgaaactgg ctcctgtcgc tggggaaagg ctttcaatgt gaccgtgaaa
     9301 cacttcccct ttgagaatat cagcacggat ctcagccttc attcctggct tcattcccgc
     9361 tatttggttt tctttataat ttgcatcaac ccacaactca ctgactggta caagtgaaac
     9421 taacgatgtg ccgccttcca cccaactgcc tgtatgggcg ctgcggtttg cgacaatgcc
     9481 atcaatcggc gcacgaacaa gggtatattc cagatccagt agtgcctgag tcaaagcggc
     9541 ctgaacttct tcgatactcg cacgtacgtt ttcttcctga gcagataaaa ctgccagccg
     9601 ttgcttctcc accaggaaat cgttcgcggc tttgcgctcc tttgctgcga cctgctggta
     9661 gtcaaacctg gcgttatcaa taatttgttg cgatattgcc gctgattgtg cgagtttttc
     9721 ataacgttca gtatctcgca ggcgcttttg ctcctcatgt tttactgcct gccatgtttc
     9781 actggcactt tgaattatcg actgctgcat cgcaatagtg gcttgaatgc tttcgagatt
     9841 ggctttgcta ctttttattt ttgcctcaag cctggcgaca tttgcacgat aatcccgatc
     9901 atcgagacgc aggataatat cgcctttttt tacgctctga ttatcgcgca cctctatagc
     9961 agaaatataa ccagaaactt tacttgccac cgtggtgata ttaccaccaa tgtaagcatc
    10021 atccgtcgtc tgtataaatc ttcctgtgct tacccaccag ataaaaaaga caacgccagc
    10081 taaaataata ccgatcgcta ccaccccaat aagctgtttt ttagaaatta tcatcactct
    10141 ttacaccgta tatatccgga aacttgcatt cgtcatattt aaaatttaac atggcttatt
    10201 tgatgatttc tgacaaaata cctactgtaa atgacaatca cactgcaacc caaagcatca
    10261 ccagggcatc acattatcgg actggattcg gaacatctga atggaggtac agttccctgg
    10321 cataaacatc tctatgccca gttgctttac ccggctgaag gagtcgttcg tgtatgggct
    10381 ggagaaagcg tctggctagt gcatgccagc agcgcgcttt ggctgccccc acaaatgccg
    10441 cataagtttg ttgcgacagg caacgtactt ttaaaaaccg tactggtttc agaagcggaa
    10501 agtgaaacgc tgggaaaagt ttgcttcatg acgggtattt cacccctgtt aagggaatta
    10561 ctaattgcaa ttaaccaact tccaccgtcg caaagtacca cagataaaca acagttacgt
    10621 ttttcagccc tcgagacact gatacttcag gaaataaaaa tgggggtgaa aatgtcactt
    10681 gaactccctt ggccaaatga tgagcggcta cagcagcttt gtgagaattt gctaaacaac
    10741 caagggtatc tgccaacatt ggataatcta gcggataaaa ttaacgtcag tagccgtaca
    10801 ctcatgcgct tatttgttaa agagacggga ttaacatttc gccactgggt tcagcaaatg
    10861 cacgttattt ctgcagtgac gcttcttgat gatggatatt cgttgaccaa aattgcccat
    10921 cgtctgggct atgcctcagc tgaatcgttc ggcaatatgt ttaagcgtcg aacgggctac
    10981 tcgcccggta aatttactcg tcgattaacg atgcatgatt atgcaatcac aagacaaatg
    11041 atttaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacgg atgggtaaaa
    11101 ccgttataac actgtcaccg gtcaccagga ccccaggccg gatttaagac gaggatgcac
    11161 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
    11221 aactttgcag atagccgcat tctcgccgaa attgcgtgat taatgacgcg gtcagtggtg
    11281 tctggcgact atcacgtcgc tgaatcaaat aataggtcgc tttcggtaaa atttcgctaa
    11341 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggtagta
    11401 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
    11461 cgccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaagtgt ttgtagtagc
    11521 tgccgtgtgg cgtcggcatt gtccagctgt agtccagtaa ctgtttgatc gaacgggcac
    11581 caatggcggg atgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
    11641 aagtaaattc gtggtcgtag ggtccctgat aataggtatt gatggtgaaa tccagttccc
    11701 cctggcgcaa ttcattaatc atcgatacca gttgcccttc cataatgcgt acttttacct
    11761 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
    11821 cccccatgcc gatattaatt tgccctgcta gttgcccttg tcgttggcga atatcctctt
    11881 gcgctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcgccggcat
    11941 cagttaacgt cacgccttta ctacggcgaa aaaagagttg tgccgccaac ccttcttcca
    12001 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
    12061 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
    12121 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
    12181 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
    12241 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
    12301 ttatggagtc tttgaatcaa tttattaatt cgcttgcccc aaaattatcg cactggcgac
    12361 gtgattttca ccactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
    12421 cggaagaatt gcaccagctc ggttattcac tggcgctggg ccgcgaagta gttaatgaaa
    12481 gtagccggat gggattacct gatgaattca ctctacaacg cgaattcgag cgcgctcgtc
    12541 aacagggtgc gctagaacaa tggattgcgg cttttgaagg cggtttcact ggcatcgtcg
    12601 ctaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
    12661 tggatctcag tgaagaacgg gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
    12721 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt ggacttgggc
    12781 tggcgcatac ccttaaacag ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
    12841 aacctgcaga agaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtagtggatg
    12901 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
    12961 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc gggaccgccg
    13021 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcaa
    13081 ctcttgcact gcatgcaatc gccccgcaca gcgaaggcgc ttccagagta aacgtgggcg
    13141 ttatgcaggc aggaagcggt cgtaatgttg ttcccgcctc agcgttgctg aaagtggaaa
    13201 cacgcggggc cagcgacgtc attaatcaat atgtttttga acgtgcacaa caagcgattc
    13261 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
    13321 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcagct caggtcccgg
    13381 gggtcaatca agccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
    13441 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
    13501 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
    13561 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
    13621 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
    13681 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
    13741 ggcttcggcg ctggagtctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
    13801 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gggaatatga
    13861 cgccctggca ggtttaagtc agcaagcaag ttgcgcgcaa cctacatccg cgacgcccgg
    13921 tgaaaatggt cacggttgcg gacacaattt gctgggaacc gccgcctttg ccgctgcaat
    13981 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acggtgcgct tttatggttg
    14041 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
    14101 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
    14161 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
    14221 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
    14281 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacag atagcggcgg
    14341 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    14401 aatgaccgat gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    14461 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    14521 tcgcacctta gaaaatgcca tgtaccgagc cctatcccat tttggtaccc cggaatggaa
    14581 ctgcgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    14641 aaacagtctg aataatatcg ctgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    14701 tcatcgtgaa acggtactgg cgaatgaagt cgctccatat gccgccaccg ataacgtgct
    14761 tgcggcatcg actgatgtcg gcgacgtcag ttggaaactg cctgttgccc agtgtttcag
    14821 cccctgcttt gccgttggta ccccgctaca tacgtggcaa ctggttagcc aggggcgaac
    14881 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacacttaa
    14941 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagttacgga
    15001 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    15061 acaacaacgc aaacacaaca aacgaggaat gcccatgagt atgtcatcca taccgtcgtc
    15121 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc
    15181 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    15241 ctttggcgtc agtgcaaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct
    15301 cagtgtggaa ggattacact ggtttttacc caatgttatt aaaaacttta gcggttttgc
    15361 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    15421 actaccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    15481 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc
    15541 accgatgggt gcgctaattt ttctggcggt gggcaggcat ccagttgcag gtttacttgc
    15601 cgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    15661 gttgctgtcg gggatcagca cggaggctgc ggctgcgttc aatccgcaaa tgcacgtcag
    15721 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    15781 cctgataacc gacaaaatca ttgagccacg tttaggtcaa tggcagggaa acagcgatga
    15841 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    15901 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    15961 tccgattaat cacaccgtga tgccatcccc ctttattaaa ggtatcgtgc cattgatcat
    16021 tctttttttc tttgtggtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    16081 ggcggattta ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat
    16141 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat
    16201 cgccgtgggg ctgaccgata tactggaaag ttcagggctt agcggcatcc cggcgtttgt
    16261 cggtctggcg ttgctttcct ctttcttatg catgtttatc gccagcggtt ccgcaatctg
    16321 gtcgattctg gcccccattt tcgtaccgat gtttatgcta cttggctttc acccggcatt
    16381 tgcgcaaatc ctctttcgta ttgccgactc atccgtattg cctttagcgc cagtatctcc
    16441 ttttgttcca ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac
    16501 ttactattcg ttagttttgc cctatccact tatctttttg gtggtatggc tgctgatgtt
    16561 gctggcgtgg tatcttgtcg gtctgccgat aggtccgggg atttacccac gtttgtctta
    16621 agagagaacg gatgctgaga ttacttgaag aaaaaattgt cacgccactg ggtccactgt
    16681 gggtgatttg cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac
    16741 gcatggtgca gctgctggac atccattatc gcaaagaagg ctatgagcgc atttctgcca
    16801 ccaacccagg cggtttaagc gacaaacttc gtgattattt tgccggtaat cttagcatta
    16861 ttgatacgct tcccactgcc acagggggga cgccatttca gcgcgaagtc tggaaaacac
    16921 tacgcactat cccctgcggg caggtaatgc attacggtca actggccgaa caattgggtc
    16981 gccctggcgc ggcgcgtgcc gttggtgcgg ctaacggatc gaatcccatc agcattgttg
    17041 tgccttgtca tcgggtcatt ggcagaaacg gtaccatgac cggatatgca ggcggagttc
    17101 agcgaaaaga gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt
    17161 tgtgccagct tgttcacact tttatgtaaa gttaccctta acaacttaag ggttttcaaa
    17221 tagatagaca tatatttaca tctaatatcg gaattctctg ctgttaaggt ttgcttagac
    17281 ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc aattacggct tgagcagacc
    17341 tatgatcccg gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg ctatccattg
    17401 ccaggattgc agcatcagcc agctttgcat cccgttcaca ctcaacgaac atgagcttga
    17461 tcagcttgat aatatcattg agcggaagaa gcctattcag aaaggccaga cgctgtttaa
    17521 ggctggtgat gaacttaaat cgctttatgc catccgctcc ggtacgatta aaagttatac
    17581 catcactgag caaggcgacg agcaaattac tggtttccat ttagcaggcg atctggtggg
    17641 atttgatgcc atcggcagcg gtcatcaccc gagcttcgcg caggcgctgg aaacctcgat
    17701 ggtatgtgaa atcccgttcg aaacgctgga cgatttgtct ggtaaaatgc cgaatctgcg
    17761 tcagcagatg atgcgtctga tgagcggtga aatcaaaggc gatcaggaca tgatcctgct
    17821 gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc atctacaacc tgtcccgtcg
    17881 ttttgcccaa cgcggcttct cccctcgtga attccgcctg acgatgactc gtggcgatat
    17941 cggtaactat ctgggcctga cggtagaaac catcagccgt ctgctgggtc gcttccagaa
    18001 aagcggtatg ctggcagtca aaggtaaata catcaccatc gaaaataacg atgcgctggc
    18061 ccagcttgct ggtcatacgc gtaacgttgc ctgatttttc cgcataactc actatccttc
    18121 tgtcatatca ttaaattttt ctgatttatt gatctggcag aaggttcatc actgtttcat
    18181 tcaccagata tgggttaatc ttttaattac aaactgcgtt gacagttgtt gtaaggagac
    18241 cctgtatggc tatgtatcag aacatgctcg ttgttatcga tcctaaccag gacgaccaac
    18301 cagcattgcg gcgagctgtt tatttacatc aacggattgg tggcaaaatt aaagcctttt
    18361 tgccgatcta tgacttctca tacgaaatga ccaccctgct ctctccggac gaacgtaccg
    18421 ctatgcgtca gggcgtcatc agccagcgta cagcctggat ccacgagcag gcaaaatatt
    18481 atctcaatgc tggcgttccc attgaaatta aagtggtctg gcataaccgt cctttcgaag
    18541 ccatcattca ggaagtgatc agcggcgggc acgatttggt gctaaaaatg gcgcaccaac
    18601 atgaccgtct ggaagcggtg atttttacgc caacggactg gcatctgtta cgcaaatgcc
    18661 caagcccggt gtggatggtg aaagaccagc cgtggccgga agggggtaag gcgctggtgg
    18721 cggtgaatct cgccagtgaa gagccgtacc ataatgcgct caatgaaaaa ctggtcaaag
    18781 agacgatcga actggcagaa caagtcaacc atactgaagt tcatctggtt ggcgcttatc
    18841 cggtaacgcc aattaatatc gcgattgaac tgccggaatt tgatccgagc gtttataacg
    18901 atgccattcg tgggcaacat ttgctggcaa tgaaagccct acggcagaaa ttcggcatta
    18961 atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg
    19021 agcatttaca ggcggggatt gtggttctgg gcacggtcgg acgcaccggt atttcagcag
    19081 cattcctcgg caacacggcg gaacaggtga ttgatcatct tcgctgcgac ctgctggtca
    19141 ttaaacctga ccagtatcag acacccgttg aactggatga cgaagaagac gattaacgac
    19201 tcttcaataa ctgacaaggc ccgagttatc gggcctattt tttacttact gctcttcgcc
    19261 gccaagaaaa taaattccca acggtattgc cagcaacaca gtaaagacca ggctataaac
    19321 aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc cactcagaca gcggaatatt
    19381 gtactgttct ataacgccgc cgatggtcgc gcgagtcaca accgacgccg caataatgaa
    19441 aaccgccaac aaaccaaaga gaaatttctt ccctttcgct gacttcagtt tcgccataat
    19501 catgatgtaa tccttgtgca gacaaatttc ttgttaccta aacgtaacaa taccatg
//
LOCUS       DARNVM010000088        19086 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.088, whole genome shotgun sequence.
ACCESSION   DARNVM010000088 DARNVM010000000
VERSION     DARNVM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.088"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(41..1264)
                     /locus_tag="SI621_002804"
     CDS             complement(41..1264)
                     /locus_tag="SI621_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065495.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQHQLQQINACHTNTTYTNADYSKIVAQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKNSECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFNAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAFT
                     TLSRYNKIQTWAEMSEYQFEVFQYETLNPKKMSRTPYLKRSLPDEKTINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(1844..2845)
                     /locus_tag="SI621_002805"
     CDS             complement(1844..2845)
                     /locus_tag="SI621_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEI4065496.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQMLF"
     gene            2953..3249
                     /locus_tag="SI621_002806"
     CDS             2953..3249
                     /locus_tag="SI621_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4065497.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(3372..3797)
                     /gene="arsC"
                     /locus_tag="SI621_002807"
     CDS             complement(3372..3797)
                     /gene="arsC"
                     /locus_tag="SI621_002807"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HEI4065498.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3810..5099)
                     /gene="arsB"
                     /locus_tag="SI621_002808"
     CDS             complement(3810..5099)
                     /gene="arsB"
                     /locus_tag="SI621_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HEI4065499.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALLTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYALVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLAENGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(5154..5507)
                     /gene="arsR"
                     /locus_tag="SI621_002809"
     CDS             complement(5154..5507)
                     /gene="arsR"
                     /locus_tag="SI621_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HEI4065500.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSEDSKNICS"
     gene            6250..6333
                     /locus_tag="SI621_002810"
     CDS             6250..6333
                     /locus_tag="SI621_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HEI4065501.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(6387..7739)
                     /gene="gorA"
                     /locus_tag="SI621_002811"
     CDS             complement(6387..7739)
                     /gene="gorA"
                     /locus_tag="SI621_002811"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEI4065502.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7811..8653)
                     /gene="rlmJ"
                     /locus_tag="SI621_002812"
     CDS             complement(7811..8653)
                     /gene="rlmJ"
                     /locus_tag="SI621_002812"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HEI4065503.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8856..10898
                     /gene="prlC"
                     /locus_tag="SI621_002813"
     CDS             8856..10898
                     /gene="prlC"
                     /locus_tag="SI621_002813"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HEI4065504.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10906..11658
                     /gene="rsmJ"
                     /locus_tag="SI621_002814"
     CDS             10906..11658
                     /gene="rsmJ"
                     /locus_tag="SI621_002814"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HEI4065505.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11707..13176)
                     /gene="dtpB"
                     /locus_tag="SI621_002815"
     CDS             complement(11707..13176)
                     /gene="dtpB"
                     /locus_tag="SI621_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HEI4065506.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(13492..13926)
                     /gene="uspA"
                     /locus_tag="SI621_002816"
     CDS             complement(13492..13926)
                     /gene="uspA"
                     /locus_tag="SI621_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEI4065507.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            14317..14652
                     /gene="uspB"
                     /locus_tag="SI621_002817"
     CDS             14317..14652
                     /gene="uspB"
                     /locus_tag="SI621_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HEI4065508.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14723..16222)
                     /gene="pitA"
                     /locus_tag="SI621_002818"
     CDS             complement(14723..16222)
                     /gene="pitA"
                     /locus_tag="SI621_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HEI4065509.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16454..17656
                     /locus_tag="SI621_002819"
     CDS             16454..17656
                     /locus_tag="SI621_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEI4065510.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(17971..19023)
                     /locus_tag="SI621_002820"
     CDS             complement(17971..19023)
                     /locus_tag="SI621_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HEI4065511.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGIPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4984 a         4660 c         4802 g         4640 t
ORIGIN      
        1 ctatttttat tcaatgctta taaagcgcta ataatcaata ttatggtatt atattctgat
       61 aatgatttga taagcgaatt cttttgccgg atggtatttc tatttccacc ccataattta
      121 tagttttttc atcaggtagc gatctcttta aataaggcgt gcgtgacatt ttttttgggt
      181 taagtgtctc gtattgaaat acttcgaatt gatattcaga catttctgcc catgtttgaa
      241 ttttattgta tcgggagagt gtcgtaaagg ctttaacttg cttataacat tgtttttgga
      301 attcttgctg atattttaat atgtcattgt atgaaagatt ggttatcctg gcctctaaga
      361 atttaattat tgtcgaagca atttccgctt ttgctcgtaa aaagttgtca ttaatggtta
      421 ataatgttga aaataaacct tcgatttgtt tgtcaagata ttgttcgtct ttatataatt
      481 ttggggtagg gtgttcttta tgtgaaaaaa tagtgtcacc taaatttgcg tcagtgaaat
      541 ttatattaag tagatcttcg aaataaaacc ttgacaaggt tagaacgaac ccggacaggt
      601 tctgtcctgt catattgaaa tcaataaagt tggttccggc aaagactttc atcctgtttt
      661 tattttcact gtgttcaata tataacttcg tcattttttg atacaggctt tgaatatctt
      721 ttatattagt cggtaagtca gctcgttgtt cagcattaaa attagtatct gaaaacatgg
      781 tttggacact gaatcttact ggtattccct cttctacgct aattttagtt atgattgcct
      841 tgtcgggttt aggattagcg acaacaatga ttcgacattc agaattttta ttcttttcgt
      901 atctaatgtt attaatctga ttaagtattg attgtgtctg ttgcgtaagt ggtgtcgaac
      961 ttatcgtttc aatgttatta ataagactta caagttgagc cacaattttt gagtagtctg
     1021 catttgtata tgtagtgttc gtgtggcacg cattaatttg ttgaagttgg tgttgcgcta
     1081 aatcatattg atcagaggta tatccttctc cagagaatgt ggaattaatg ggttgttgtt
     1141 cttcccaatt atcgcaaggc cacttcggtg tatctggcgt tttagcatta tcggtatttg
     1201 ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta aagtcaattg
     1261 acataaaatt ccttattcat catttgtttt atgctaagat gtttaaattg gataattaga
     1321 acaggaatgg attttcttct acacacaata aatgtaatgc gttatctctt cctgatataa
     1381 ttacctttga tttccctttt atctccaccc ggtaatattt gtgtataaaa aaagggcagt
     1441 gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag cgctcatttt aaaaagttat
     1501 gatgttcacc gatagtgtgt gaagtgatat ttttgtgatg ttttagatgt gtaataatga
     1561 accaggttaa agtgtattta aaaatgtgtt gtattggcga ctgaaatctg ccctttaaaa
     1621 gggggatagc actgaaacaa agagacattg cttctccgat aaaatgtcag agaagcatga
     1681 ttgcgtggtg attgtcgatt gatgacttac tgacggatcc gtcatcattc tcaaagcatt
     1741 agttaagtta tttgtgattt ttaaggatta tcctatctat gattttctac tcagaaggat
     1801 aaggcactca caatgctcct tatagtttac tgctggcatc tatttaaaat agcatttgag
     1861 tcaaatagcc catcaagata gccataccta agataaccgt caggaatgca gctatcatcg
     1921 gcattctgaa cattgattta agtaatatca cttccgtcag gcttgctccg gcactgccga
     1981 tgattaatgc cattaatgct cccagcccca ttccttttgt catcagaaca gatgccagag
     2041 ggataaccgc ctctgcccgg atatacagcg gaattccgac aacggcgctc aatgggatgg
     2101 cgaagggatt atctgcccct gcatgagcgg caatccactc tgagggaata aagccataaa
     2161 taaaagagcc tattaatacc cctaacaaaa gatatggcag tacatcttta aactgttgca
     2221 acgcatcttt ggtggctagt tttatgggat taatacttac cacagctttg gtactgcaac
     2281 agtttactac aggtttctca atggctttag ccgctggact acagcaactc acttttattg
     2341 gcgtatatgt cgtccccggc gaagttttgg ctggagcaca acaatttgct gatgagcttt
     2401 tactggcaat gatatgacgt tcaaatccca gggaatccag gataatactg gcaagtacgg
     2461 agacgccggc ggcgataatc gcgtacaaca aggtaacttt ccagccaaag gtcacccaca
     2521 ttaacccgac gataatggga ttaagtaatg gggaaacaaa taaaaaagtg agagtcggac
     2581 caaacccggc tttcgctgat aacaatccac gtaacatggg gattgtcgag caactacaga
     2641 acggggtaac ggctcccaac agagcagcca ggagatagcc tttccctttt cttgccccca
     2701 tcatctgctg gattttatgg tctggcacct tttgtcttat caggctgaca ccggcactaa
     2761 tcactataaa cagcaaagaa agctcgacgg cgagaaacac aaacatctct gcggcatctt
     2821 gcagcatcgc aagccaacta ctcatacgta acctcattga atgaatccat atttccagaa
     2881 taatcgaaat gaaattcaga tcaagatatt tctggtatta tcgaaatatc aaccagacaa
     2941 agggggtgtc atatgcagtt ggaagaagtt gctaaagcgc tgaaagagct aggtcacccg
     3001 acccgtttgt tcatattcaa acacctggtc aaagcggggg aacaaggttt gccggtcggt
     3061 gagttgcaaa agcagcttgg gatccccggc tccacactca gtcaccatat ttcagcactg
     3121 gtatcggtag ggctggtcaa acaaaaccgt gagagtcgaa cgctgatgtg tgtttcgcaa
     3181 tacgcaatgc tggaagcgat aattgaattc ttacgtgagg agtgctgcgt aaatagcaaa
     3241 attgcctgat gcgctacgcg ctacagattt catgccatgc acggttttgt aggtcggata
     3301 agacgcgtta gcgtcgcatc cgacataaac aaagcgcact ttaccaacta tgggcagtgg
     3361 atatcgccgc tttatttcaa gcgtttaccc gcttcatcaa ccactttctc gccatcttcc
     3421 ttggtgaacg cgccttgttg cgcatctggc agaatttcca gcacgacttc tgaagggcgg
     3481 cacaggcgag ttcccagcgg cgtcaccaca atcgggcgat taatcagaat cgggtgctga
     3541 agcataaagt cgattaaccg atcgtcagta aatttatctt ctgcaaggcc cagttcctca
     3601 tacggctcga cgtttttacg cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt
     3661 ttgaccagtt catcacgcgt cggtggggtt tccagataat agacaatggt cggttctgtg
     3721 ccgctgttgc ggatcatctc cagcgtattt cgcgacgtgc cgcaggctgg gttgtgataa
     3781 atggtaatgt tgctcatatc aatatctcat tacaaagtga aagagagacg tagcgccagc
     3841 gcagccagcg tcacaaaaag caccggcagg gtcatgataa tccctgtacg gaagtaatat
     3901 ccccagctga tagtcatatt cttctgcgaa agtacgtgca gccagagcag cgtcgccagg
     3961 ctaccaattg gggtaatttt cggccccaaa tcgcagccaa tcacgttggc ataaaccatc
     4021 gcttctttga taacgccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc
     4081 gtcggcatat tgttcataat agaagagagg aaggcggtga ggaatccggt gccgagcgtc
     4141 gcggcccaca ggccgttttc cgccagcacg ttgagtacgc cagaaagata ttccgttagt
     4201 ccggcattgc gcaggccata aaccaccaga tacatgccga gcgagaagat gacaatctgc
     4261 cagggcgcac cgcgtagcac cttacccgta ttaattgcat gaccgcgttt agcgacggca
     4321 aataatatca gcgcgcccac tgccgcaatg gcgctgaccg gaatgccgag cggttccaga
     4381 acgaaaaatc ccaccagcag aagcagtaaa acaacccagc cagttttgaa cgtagcagga
     4441 tctttaatcg cttctgcggg agatttcagc agcgccatgt cgtagttctg cggaatatct
     4501 ttgcggaaat agagatgcaa catcaccagc gtggcaacaa tcgcggcgat atccaccggc
     4561 accatcacca aggcatattc gcgaaagccg aggccaaaga aatctgcgga aacgatattc
     4621 accaggttgg agacaataag cggcaggctg gcggtatcgg caatgaaccc ggccgccatc
     4681 acgaacgcaa gcgtcgtgcc tttactgaac cctaaagcca gcagcatggc gatgacaatc
     4741 ggtgtcaaaa taagcgccgc gccatcattg gcaaacagag ctgcaacggc agcaccgagc
     4801 aggacaatcc aggtaaacag caagcgaccg cgtccattgc cccagcgtga gacgtgcagc
     4861 gccgcccatt caaaaaagcc ggactcatcc agcagcaggc tgatgataat gacggcaata
     4921 aacgccgccg tcgcgttcca gacgatattc cacaccaccg gaatatcacc cggatgaacc
     4981 acgcccgtaa gcaacgccag taccgcgccg agcgttgcac tccagccgat gcctaaacct
     5041 ttcggctgcc agataaccaa tacgatggtc aggacaaaaa tagcgcctgc cagtaacata
     5101 atgcctcccg gataaaacac atctgaaaat tcatatgtgt ttagataaat tttttaacta
     5161 caaatgttct tactgtcttc ggaacaattt tgtcgagcca ggttgcggac aatcgcctga
     5221 accttttcct gttcacatcg ccaggcctca tcaataattt tcgcggccca tgatggaata
     5281 tgcggtgata agcggtaatg aacccactta ccctgcttgc gatccagcaa tagcccactt
     5341 tcacgcagca atgccaggtg gcgggatatc ttgggctgcg actgatcgag ggcagtacag
     5401 agatcgcaga cgcataattc tcccagctcg ctgagcagta aaacgatgcc cagacgggtt
     5461 tcatcagcaa gaattttgaa cagttggatg ggtaacagaa atgacatatt gcgctcctga
     5521 ttgttgcagg tagtgtctct cttcgaagcg gataagtcaa aaacatatat gacttaacga
     5581 atgtgtaagc gcagaagaag gtaataggtg tgaattttga gttgggtatt catttgaaag
     5641 gaggtctgaa tcaggacgta aaaaaaccga ctttgcgtcg gttttttact ttccagccct
     5701 gagttggtgg ctctggccgg agtgagaatg cccacatcta acggttcaat actattagcc
     5761 agtggctaac attgcaagcg ttagcgattt cttcagctgg cgaagggaca gtttgctata
     5821 catgttggtg aaagaggatt tatatacttt catcgggtcg ctgccgtacc aggccttgtc
     5881 caaatccgga cgtaaaaaaa ccgacttcgc gtcggttttt tactttgcag cccagagttg
     5941 gtggctctgg ctggagtgag aatgccatca tctaacgcat caatgctaat accatactga
     6001 aactattgca aggacatgct ggttttataa cctgtatgta ctgtatgatt atccagttag
     6061 ttctgaggca ttttcactct ggtaatgcgc ataaacgctt tcaaagtcct ggtcagaagt
     6121 acgggtggtg ccgttaactg atgctctggt cggagtgaga atgcccacat ctaacaatgg
     6181 ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg
     6241 agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc gctactggaa
     6301 ctgatccgct tcctgcttca gcttctgaac tgatagcgga aacgtagtta agggcaaaga
     6361 gcacaccact ctttgccctt taacatttaa cgcatggtca cgaactcttc tgccgccgtc
     6421 gggtgaatgg cgacggtatt gtcgaagtct tttttggtcg cccccatctt cagcgccacc
     6481 gcgaagccct gcaacatttc atccatacca aaaccaatgc cgtgaattcc gacaatcttc
     6541 tcttccggtc caacgcatac cagtttcatg cggcacggct ggcggtgagt ggtgacggcg
     6601 gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc gtactgctcg
     6661 cgcgcctgcg gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc
     6721 ggaatgttgc tgtaatccag atgctcatca ggcttgttat taaacaggcg ttcagagaga
     6781 cggcgacccg ctgcaactgc caccggtgtc agttccactg cccccgtgtt atcgcccaca
     6841 gcgtaaatac cttcaacgtt ggtattttga tatttatcga cgacgatata gcctttttcg
     6901 ttagttttaa cgccagcggc ttccaggttg atgttgtcat tggcaggctc gcgaccaatc
     6961 gcccaaatca ggcaatctac cgtttcactg cgaccatctt ccagctccag cgtcaggctg
     7021 ccgtcggcat ttttcaccac cgctttcggg atggcgttgg tgtgcagctg cgggccttcg
     7081 gcgttcatca cttcgaccag cgtttcagag atcatcgggt cgaagctgcg cagcggcgca
     7141 tgtttacgca caaacagatg cgttttcgcg ccgaggccgt taatcacgcc cgccagctca
     7201 acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag
     7261 aagccatcag aatcaatacc gtattccacg cccggaatat ccgggtggct cggacgaccg
     7321 ccggtggcga tcagaatatg atcggccgtg atggtttcgc cgtttacctc cagcgttttg
     7381 gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt
     7441 tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag
     7501 ttgaatttat ttatagtggt atcgaaacca taatccggac cgtacatgtg gatcgcttca
     7561 cggatttgcg ccgcgtgcca catcactttc ttcggtacgc agccgacgtt cacgcaggtg
     7621 ccgcccagct ctttggcttc aatcagcgcg catttctggc cgtacatagc cgcgcggttg
     7681 atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg
     7741 acagagtgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca
     7801 atggctgcaa ttactccggc acgatccagc ttacggtggc gtgcccggtg cctgccggaa
     7861 ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagc ttccacggcg
     7921 ggttaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagtaccg
     7981 ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc caggtcgtgg atcatgcgct
     8041 taatttgctg gcgcaacacc actggatacc acagtgcgta agtgccagtg gcgaaacgtt
     8101 tatagccttc tgctatcccg ctgaccaccg cctgatagtc ggttttcatt tcatacggcg
     8161 ggtcgataag gattaaacca cggcgggaaa ccggcggcag tttggcctta agctgctgga
     8221 aaccatcggc tttttcgacg cgcgcacgat tgtctttctg aaattcagaa cgtagcaacg
     8281 ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc ctgttcacgc agtaactgac
     8341 gagcaatcaa cggcgaaccg gggtagtaac gcaactgacc gctacggttg aagtgtttta
     8401 ccacattgat gtacgcctcc agctctgcag gcaaatcgtc ctgctgccag atacgggcga
     8461 taccttcgag atattcgccg gtacgctcgg catgttcgct gcctaactga taacgcccgg
     8521 cccctgcgtg ggtatcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga
     8581 tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga aagctgtggc
     8641 gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc
     8701 acaaaagcct atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt
     8761 ctcattgaaa ttcactacac ttaaccccat gctacaaaca ttatgtaaag cgcctgttga
     8821 gcgcttcctt aacctcttta accaggactg cgctaatgac gaatccgtta ctgactccct
     8881 ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg
     8941 cgctgaacga ctgccgcgaa aatgtggagc gcgtagtagc gcaaggggca ccgtacacct
     9001 gggaaaatct ctgccagccg ttggcggaag tggacgatgt gctggggcgt atcttctccc
     9061 cggtcagcca cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg tacgaacaaa
     9121 ccctgccgct gctgtcagaa tacagcacct gggtaggaca acatgaaggg ctgtataaag
     9181 catatcgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggcg cagaagaaag
     9241 cggttgataa cgcactgcgc gactttgaac tctctggcat cggtctgccg aaagagaaac
     9301 agcagcgtta tggtgagatt gccactcgtc tttccgagct gggcaaccag tacagcaaca
     9361 acgtcctcga tgcgacaatg ggctggacca aactcgttac cgacgaagcg gagctggcgg
     9421 ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc tgaagcgaaa gaacttgaag
     9481 gctacctgct gacgctggat atcccaagct acctgccggt aatgacctac tgcgacaacc
     9541 aggctctgcg cgaagagatg tatcgcgctt acagcacccg cgcctccgat caaggtccga
     9601 acgccggtaa gtgggacaac agcaaggtga tggaagagat cctcgcgctg cgtcacgaac
     9661 tggcgcaact gctgggcttt gaaaactacg cctttaaatc ccttgctact aaaatggcag
     9721 aaaacccgca gcaggtgctg gatttcttaa ccgatctggc aaaacgcgcg cgtccacaag
     9781 gcgaaaaaga gctggcgcaa ctgcgtgcct tcgctaaagc cgaatttggc gtggatgagt
     9841 tgcagccgtg ggatatcgcg tactacagcg aaaaacaaaa acagcacctc tacagcatca
     9901 gtgacgaaca gctgcgtccg tacttcccgg aaaacaaagc ggttaacggc ttgtttgaag
     9961 tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc
    10021 cggatgtacg tttcttcgaa ctgtatgacg agaacaacga actgcgcggc agcttctacc
    10081 tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgtgtaggcc
    10141 agatgcgtaa agccgacggt tcgctgcaaa aaccggtcgc gtatctgacc tgtaacttca
    10201 accgcccggt aaatggtaaa ccggcgctgt ttacccatga cgaagtgatc accctgttcc
    10261 acgagttcgg tcacggcctg caccatatgc tgacccgcat tgaaactgct ggtgtatctg
    10321 gtatcagcgg ggtgccgtgg gatgcggtcg aactgccgag ccagtttatg gaaaactggt
    10381 gctgggagcc tgaggcgctg gcatttatct ccggtcacta tgaaaccggc gaaccgctgc
    10441 cgaaggagct gctggataaa atgctggcgg ctaagaacta ccaggcggcg ctgtttattc
    10501 tgcgccagct ggagttcggc ctgtttgatt tccgcctcca tgccgagttc cgcccggatc
    10561 agggagcgaa aatcctcgaa actctggcag aaatcaagaa actggttgcc gtagtaccgt
    10621 ctccatcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgccg
    10681 caggttacta cagctacctg tgggccgacg tgctggcggc agatgccttc tcgcgctttg
    10741 aggaagaggg cattttcaac cgtgaaaccg gacagtcgtt cctcgacaac attctgagcc
    10801 gtggcggttc agaagagccg atggatctgt tcaaacgctt ccgtggtcgt gaaccgcagc
    10861 tggatgcgat gctggagcat tacggcatta agggctgatc attcagtgaa aatctgctta
    10921 attgatgaaa caggcaccgg agacggtgcc ttatctgttc tggcggcccg ctgggggctg
    10981 gagcacgatg aagacaacct gatggcgctg gtattaacgc cggagcatct ggaactgcgc
    11041 aagcgtgatg agccaaaact tggcggcatc ttcgttgatt ttgttggcgg agcgatggcg
    11101 caccgacgca aattcggtgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa
    11161 ggcgattatt tgccggatgt cgtcgatgct accgcagggc tggggcgcga tgcctttgtg
    11221 ctggcttcag tcggctgccg cgtgcggatg ctggagcgta atccggtggt tgctgcattg
    11281 ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg gttgcaggag
    11341 cggttgcagt taattcacgc ctccagcctg acggcgctga ccgatattac cccacgcccg
    11401 caggtggtct atctcgatcc gatgttcccg cataagcaga aaagcgctct ggtgaaaaaa
    11461 gagatgcgtg tctttcagtc gctggtgggg ccggatcttg atgccgatgg attactagag
    11521 cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc gcccggacta cgcgccgccg
    11581 ctggcgaatg tcgccacgcc aaacgcggta gtgaccaaag ggcatcgctt tgatatttat
    11641 gccggaacgc cggtgtaaaa gtaaagcagg gtagcgttat cgctaccctg ctttctgcaa
    11701 gaataattaa tggctttcgg gcgtcgcaat cattcgtttc agccacggca ccatcagcag
    11761 catcaccacc gcaacgccca gcgtaaccag gccaatctta ccaaacacgt tggtatagac
    11821 gggcaacgtc tcaagcggat cggtaatgtt gtctggtact gcggtaaatg ttgccacata
    11881 gccgcccagc aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat
    11941 caaatgctgc ggcaccaggg cagcaatcat cgccagaccc agggcgctaa taaacaattc
    12001 acccaggctc tggaataagt aaaccagcac aataaaccac ggcgatgtca gtccttgggc
    12061 atccgcaaac cacattcccg cagctgcagc cgtcaaaaag cccagtgagc acataaacat
    12121 gccgagagta aatttcatcg gcatagagag gtctttgcct ttgttaccca gatgcgtgta
    12181 aatgcctgcc agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct
    12241 gaccgggttg atggaaaagc cgagaatttc atgatgcacg ttgttgatgg caaagaagtt
    12301 cagcgacgtt ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac
    12361 aaaggcgaca aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa
    12421 aatgatggtg acgacgatag agagaacaat cagcaccaga ttggcgactt ctacgttgtg
    12481 catcagccat gcacagacga agatcatcac cacgctgcca agtaacacgt acaacagttt
    12541 gctgaagctc atcggccgga agtcgggttc agaaccaatg tctttcacca ttccgcgaca
    12601 ggcgatgtaa accaatagcg cgataattaa ccccgcaccg cacaggttgt aggtgactga
    12661 ataaccgaat ctatcagcga tcacgggggc cagcgataac gctatcaacg agccgatgtt
    12721 gatcgacata tagaacaggg taaatgcgcc atcaagccgc ggatctttcg gcggatagca
    12781 cttcgaaagc aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc
    12841 cagggcgatg aaaatcaggt caggcttaag tagcgacagg ccggtcatga agtagccaat
    12901 cgccagcaca agtgctccga gaacaatggt gcgtttggtc cccagcaggt ggtcgccgac
    12961 atagccgcca atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa
    13021 agcctgttct tgcgagaatc caagctgctt aacgaagaaa accgccagta cgccctgcac
    13081 gccgtagtag ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg
    13141 ttgctgcagc atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc
    13201 ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag ttttcatctg tcagagattc
    13261 ttaatattga tgcggtgaat agattaatac actattacaa cagaaaataa ccagctgatc
    13321 acactaatga tggaaaatat gatggtgtta aatgttgtat tggcagggga ttttgattgc
    13381 gtacgcagac cgtaggccag ataaggtgtt tacgctgcaa ggtaacaagg cgcgccaaaa
    13441 agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tagagggaag attattcttc
    13501 ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc
    13561 ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc
    13621 gtatttcttg attgcatcga ccagaacctg gcccagatcg ccgctgccgc tcagggtttc
    13681 agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga
    13741 gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta
    13801 gtttacatct acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc
    13861 tttctctacc agaactttgc tttccgggga gagatcgacc gcgatgagaa tgtgtttata
    13921 agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg
    13981 ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt
    14041 cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt
    14101 acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa
    14161 aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa
    14221 gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg
    14281 ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt
    14341 tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg
    14401 ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc
    14461 ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc
    14521 caacgttatc gcgatcatca cgatgatgag tttattcgcc gctgtgagcg ggtgcgtcgg
    14581 cagtttattc tgaccagcgc attgtgtggt ctggtggtgg tcagtctgat tgcattgatg
    14641 atttggcatt aagcacttca gcaaaaataa cgcataaaaa aagcgggcca gtaaactgac
    14701 ccgctcgttt tggtgcgtgc gattacagga actgcaagga gagccagtaa agcccgccgg
    14761 aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatactg gtcacggttt
    14821 tacgctgtaa gccgccgcca tctaccacca tcgtccccgc gacagaagag gagagtacgt
    14881 gggtagtgga aaccggcatc ccggtataac tcgccaggcc gatagacact gccgcagtca
    14941 tctgggcaga catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag
    15001 tcgttgccac acgacgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca
    15061 tgatgatcca caccggcgca tactcgatgg tgctaagcat atcggatttc agtttcttca
    15121 acaggcgctg atcgtcagca ctcacgccag gcatcttcac cactttgtcg atagtgtcag
    15181 aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc
    15241 tttccacatc ggtagtcaac atgcctttca ggcggttgag cgcgttaatg gtattcgacg
    15301 gatggcaatg gaactccgca ggttgcgttg cgccagcttc cggagccggt actaactgat
    15361 cagcaccggt agcctgtttc agcagcgcag ggtgctgctc aaagtaagct tccacgttgt
    15421 tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc
    15481 ctgctggtgc gacgccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt
    15541 tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcaata cgcgtccaga
    15601 acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg
    15661 cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca
    15721 ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat
    15781 tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga
    15841 tcgcgccaat cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt
    15901 tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca
    15961 tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac
    16021 ccagcaaaac acccaaaaag ttgaacaccg ccgccataac cacggcgagc tgagaacgca
    16081 tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg
    16141 cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca
    16201 ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca
    16261 ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc
    16321 tgtatggatt tcactcaaaa aataattatc ttatataatt caggtaaata cttcctttta
    16381 gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc gttttttctt
    16441 agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat
    16501 gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa
    16561 aaaaccaggg cgcaaaatcc tcatgtctgg cggtgggcgc tgcaacttta ccaatcttta
    16621 tgtcgaaccg ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg
    16681 ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa
    16741 aacgttaggg caactcttct gcgatgactc cgctcagcag attgtcgaca tgctggtgga
    16801 tgagtgcgag aagggcaatg tgaccttcag attgcgtagc gaagtgctga gcgtggagaa
    16861 ggatgataca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctagt
    16921 catcgcgacc ggtggactgt caatgccggg gctgggcgcg tcgccgtttg gttataagat
    16981 tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct
    17041 gcataaaccg ttgctcgaag agttacaggt gctggcaggc gtggcggtgc cttccgtgat
    17101 taccgctgaa aacggcaccg ttttccgtga gaacttactc ttcacccacc gcggcttatc
    17161 tggaccggcg gtgttgcaaa tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa
    17221 tctgctaccg gatgtggacc tcgaaacctt cctgaatgag cagcgtaatg cacatccgaa
    17281 tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg aacgtttaca
    17341 acaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc
    17401 actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac
    17461 tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc
    17521 gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg gacgtcaccg gttggctggg
    17581 gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc
    17641 agcgaagtcg tcctgagcta tttcctaaag atgaaaatta ttagagtcgc attaaaaatg
    17701 agctaatttt gatagtggtt atcttgtgat tattttctaa tgagcctgtg aactgagacc
    17761 ctccatgctt aatataaggt ggagggaaag gtgattaaaa ctcccccagt ggccagatct
    17821 ttataaaata tgaacaggtt gaaaaaatca gcaacttagc tgtttattta tacttaacca
    17881 ctatgcatta atgctgcgtt atttcatgat acctaagaaa aaccagcgtt acgcaaataa
    17941 tcaacgctgg ttttatccgg tacgttgcaa ttatttttta gcagaacccg cttctaatat
    18001 tgaaacgatt gagaacaacg taaagcatac cgctcccaaa ccgaccagaa cccgcgaagg
    18061 aataaaatat cccatatcgg tctgcgccat ttccgcaaga aacgatgcca ggaaaaggca
    18121 aaacaggcag gtgaagacgg gaatcatcgg tattctgttg gctaacgaac aggtacgtct
    18181 ccagaccagt gccagtagcc agacttttga gaatatgctg taacagatca ttccgaggca
    18241 aatgaggata atgccgggag ccagtcgggc gcttgcatcg gagctgacta acacgtatat
    18301 gccctgcagc accgtgattg agccgagaat aataacccaa taacaccaca gccagtgttc
    18361 tttggatgag aacgtattac gtgtttgatg gacgattgtg gcaacaaggc caattagaca
    18421 ggcacagatt gcggttaacc caagcaatac gtgaccggcg acataatgcg gagttatgtc
    18481 ggcactacgt aacagcgtaa tagaccaaat gaaaccgagc agggtgagta acacgggaac
    18541 ggcaatgagg caattaccga ttaatgacga ataagcctgt accggtattc catcgctctt
    18601 gctccctgct gcatttttgg gaatgagcag aaagtgaccg gatgacgctg ccactgtcga
    18661 tacacaggcg gcaatcatac caacgccaaa aataacatgg ccggcaacaa actcgtctgc
    18721 catcacatca ttgcctgcta acagtgccca accccatatc atggtgataa ttgaccccgc
    18781 atagccaata atggggaaca atgtattgta aaacgtatta acgccgcgcg tgagctgcga
    18841 aatgataata aatgcggtag tgaataatgc caggcatatg gctgccagag aaatcagcac
    18901 atgacccgcg acgaaatact cactttgccc agaactttcc agcacaaagc cacctaacgc
    18961 gatacaaatt aagcccataa ttaaagggat taatttgaaa agccacccga tatatatgtt
    19021 cactttaaac tcctcaggta tccctgctaa tattgaaatg tgcattaatg ataatttcag
    19081 cctgtt
//
LOCUS       DARNVM010000089        19028 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.089, whole genome shotgun sequence.
ACCESSION   DARNVM010000089 DARNVM010000000
VERSION     DARNVM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.089"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(173..1822)
                     /gene="pgi"
                     /locus_tag="SI621_002821"
     CDS             complement(173..1822)
                     /gene="pgi"
                     /locus_tag="SI621_002821"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEI4065512.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVNYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2347..3696
                     /gene="lysC"
                     /locus_tag="SI621_002822"
     CDS             2347..3696
                     /gene="lysC"
                     /locus_tag="SI621_002822"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEI4065513.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3949..4221
                     /locus_tag="SI621_002823"
     CDS             3949..4221
                     /locus_tag="SI621_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HEI4065514.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4222..5094)
                     /gene="rluF"
                     /locus_tag="SI621_002824"
     CDS             complement(4222..5094)
                     /gene="rluF"
                     /locus_tag="SI621_002824"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HEI4065515.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5442..6389
                     /locus_tag="SI621_002825"
     CDS             5442..6389
                     /locus_tag="SI621_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEI4065516.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQVQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6459..7265
                     /locus_tag="SI621_002826"
     CDS             6459..7265
                     /locus_tag="SI621_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4065517.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7275..7682
                     /locus_tag="SI621_002827"
     CDS             7275..7682
                     /locus_tag="SI621_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HEI4065518.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPDENAGDIV
                     AKLEKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            7682..8176
                     /locus_tag="SI621_002828"
     CDS             7682..8176
                     /locus_tag="SI621_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEI4065519.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8242..9039
                     /locus_tag="SI621_002829"
     CDS             8242..9039
                     /locus_tag="SI621_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEI4065520.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTQGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATTSTALDQLDD"
     gene            9050..9874
                     /locus_tag="SI621_002830"
     CDS             9050..9874
                     /locus_tag="SI621_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEI4065521.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9926..11155
                     /gene="sorE"
                     /locus_tag="SI621_002831"
     CDS             9926..11155
                     /gene="sorE"
                     /locus_tag="SI621_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HEI4065522.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11537..12226
                     /gene="pepE"
                     /locus_tag="SI621_002832"
     CDS             11537..12226
                     /gene="pepE"
                     /locus_tag="SI621_002832"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEI4065523.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12316..13947)
                     /locus_tag="SI621_002833"
     CDS             complement(12316..13947)
                     /locus_tag="SI621_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEI4065524.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14167..17850)
                     /gene="metH"
                     /locus_tag="SI621_002834"
     CDS             complement(14167..17850)
                     /gene="metH"
                     /locus_tag="SI621_002834"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEI4065525.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18050..18874
                     /gene="iclR"
                     /locus_tag="SI621_002835"
     CDS             18050..18874
                     /gene="iclR"
                     /locus_tag="SI621_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HEI4065526.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4516 a         4947 c         4973 g         4592 t
ORIGIN      
        1 agttaaaata aaatcaaaaa taatcaacct acgtattttc cgaaatcaat cacgattaaa
       61 aatttatttt aggattaacc tcacagcatg atttccgtta aatcacagac aaggggattt
      121 atctgataaa aaatgccgac ataacgtcgg catttttatt aaggttattt aattaaccgc
      181 gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg ctgatttctt
      241 tatcatcttt cagctctggc agaatacggt tcgccagttg tttacccagc tccacgcccc
      301 actggtcgaa ggtgaagatg ttcagaatca cgccctgagt aaagattttg tgctcataca
      361 gcgcaatcaa cgcacccagg ctgaacggag tgatttcgcg cagcaggatg gagttggtcg
      421 ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc ggatctttac
      481 cctgatcacg atattcctgc tcaaccactt cgcgggattt accaaacgcc agcgcttcgg
      541 tctgagcaaa gaagttagac agcagtttct ggtgatgatc agagagcggg ttatgggtga
      601 tagccggggc gatgaaatcg cacggtacca ttttggttcc ctggtggatc agctggtaga
      661 acgcgtgctg accgttagtg cctggttcac cccagataat cgggccagtc tggtaattca
      721 cgacgttacc gttacggtca acgtacttac cgttggactc catattgccc tgctggaagt
      781 acgccgcgaa acggtgcata tactggtcat acggcagaat cgcttcagtt tccgcgccaa
      841 agaaattgtt gtaccagatg ccaatcagcg ccagtagtac aggcaggttt ttctcggcag
      901 gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca acgaagttat
      961 caaagccgat ggagagaaca atcgacaggc caatcgctga ccataaagag taacgaccac
     1021 cgacccagtc ccagaactcg aacatgttgg cggtatcaat accaaactcg ccaacggctt
     1081 tggcgttggt tgagagcgcc gcaaagtgtt tcgcaacgtg tttttcatca ccagccgctt
     1141 tcaggaacca gtcacgcgca ctatgggcgt tggtcatggt ttcctgagtg gtgaaggttt
     1201 tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact tccgcgatgt
     1261 gagtcccatc gacgttagaa acaaagtgca tgttcaggtg gtttttgtac ggacgcagtg
     1321 cttcggtcac catgtatggg ccgaggtcag aaccgccgat cccgatgttc actacgtcag
     1381 tgattgcttt gccggtataa cccttccact caccggaaat aatcgcttgc gagaaggttt
     1441 tcatcttctc cagcaccgcg ttgacttccg gcataacgtc tttgccatca accagaatcg
     1501 gggtattgct acggttacgc agcgctacgt gcagcacggc gcggttttcg gtgcggttga
     1561 tcttctcacc agagaacatc gacttaatcg cgcccgccag atcgcactct ttcgccagat
     1621 cttgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc accagcatct
     1681 gatcgtcgaa ggttgcggag aacttagaaa aacgatcgcc gtctttagca aaaagatcgg
     1741 cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag gcagcggtct
     1801 gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt gtgactttgg
     1861 aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag cgtaatgttg
     1921 agtgtaaacc cttagcgcag tgaagcattt attagctgaa ctactgaccg ccaggagtgg
     1981 atgaaaaatc cgcatgaccc catcgttgac aaccgccccg ctcacccttt atttataaat
     2041 gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg
     2101 agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa cggctggcca
     2161 cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc gttcgcagtc
     2221 gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt atcgctctgc
     2281 gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc ctgacacgag
     2341 gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc tgattttgac
     2401 gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtgcgttt agtagtcctc
     2461 tcggcttctg ctggtatcac taatctgctg gtcgctttag cggaagggct ggaacctggc
     2521 gagcgattcg aaaaactcga cgctattcgc aacatccagt ttgccattct ggaacgtctg
     2581 cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaacat tactgttctg
     2641 gcagaagcgg cggcgctggc aacgtctccg gcgctgacag atgagctggt cagccacggc
     2701 gagctgatgt cgaccctgct gtttgtcgaa atcctgcgcg aacgcgatgt tcaggcacag
     2761 tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc agagccagat
     2821 gtagccgcgc tggcggaact ggccgcgctg cagctgctcc cacgcctcaa tgacggctta
     2881 gtgatcaccc agggatttat cggtagcgaa aataaaggtc gtacaacgac gcttggccgt
     2941 ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc tcgtgttgat
     3001 atctggaccg acgtcccggg catctacacc accgatccac gcgtagtttc cgcagcaaaa
     3061 cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg tgcaaaagta
     3121 ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccggtctt tgtcggctcc
     3181 agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg
     3241 ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag cctgaatatg
     3301 ctgcattctc gcggcttcct cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg
     3361 gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac caccggttca
     3421 acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctttc cgcactgtgc
     3481 cgggtggaag tagaagaagg tctggcgctg gtcgcgttga ttggcaatga cctgtcaaaa
     3541 gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat tcgcatgatt
     3601 tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag
     3661 gtggtgcaaa aactgcatag taatttgttt gagtaaatac tgtatggcct ggaagctata
     3721 tttcgggccg tattgatttt cttgtcacta tgctcatcaa taaacgagcc tgtactctgt
     3781 taaccaacat ctttatcgga gaataattgc ctttaatttt tttatctgca tctctaatta
     3841 attatcgaaa gagataaata gttaagagaa ggcaaaatga atattatcag ttctgctcgc
     3901 aaacgaattc ccgtacactg aactctctgg ctcactgagg gcaccagcat ggcactcccc
     3961 cgtattaccc aaaaagagat gactgaacgc gagcagcgtg aacttaaaac actgctggat
     4021 cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg aaaccaacag cattaagaaa
     4081 gagtacatcg ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta
     4141 aagaaaaagc aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg
     4201 tcgacgcgcg gcaggcgctg attagcgccc cttcttttta cgccccggcg aggtaaaacg
     4261 cttaccgttg gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg
     4321 acgtttaatg cctacagttt tcggtttggc cttcgcttta ggttttgcct cggaagagga
     4381 attttcaatg agcttaaaga ggtcgattaa ctcatcgtca gttaaatcac gccattcccc
     4441 cagcggaatg ccgcttaaac taacgttcat gatgcgcgta cgttccagct ttttcacttc
     4501 gtaaccaaag tgctcgcaca tgcgacggat ctgacggttc agcccctgca ccagggtaat
     4561 gcggaagaca aacggcgctt cttttttgac tttacacttt ttcgtcaccg tcccgaggat
     4621 cggcactccc gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac
     4681 cagatactct ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg
     4741 attggtgagg aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg
     4801 tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac
     4861 aataccgacg ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat
     4921 caactgaccg tttactttca caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg
     4981 cttgccatta aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca
     5041 aattccgctt tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc
     5101 tcctgtaaaa cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa
     5161 cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga
     5221 tgcaaatttg cctggcaaaa taaggataat caacatatta cgaaccgctg cacacctgtg
     5281 ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca
     5341 aagagtgaaa aatcgctttg aaaggccatc atgatggggt tatatcagat caagcggtga
     5401 gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata
     5461 tccgtttgat tgtgaagatt gcccaactct attacgaaca ggatatgacg caggtgcaaa
     5521 tcgcgcgcga actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg
     5581 atcagggaat tgtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc
     5641 agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg
     5701 atgaagagac tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc
     5761 tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa
     5821 acttgccgca ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt
     5881 ccggtaaact cgaaagccgc tatcacgtaa acacattaac ctacagcgcg gcagcgaagc
     5941 tgaaagggga atcgcatctc gcagattttc cggctctgct ggataacccc ttaattcgta
     6001 atgggatcat gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggatg
     6061 tcgccctggt gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt
     6121 atggtggtga agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc
     6181 gcttttttga tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acactctcta
     6241 tcgaaatgaa taaattaaag caggcacgat attccattgg cattgccatg agtgaagaaa
     6301 aatacagcgg aattgttggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata
     6361 gcagcacagc tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat
     6421 tacggcgggt ccctattatc taaaaacagg agtctgttat gcaaacgtgg ttaaatttgc
     6481 aggataaaat cattatcgtc accggcggcg catccggtat aggtctggcg attgtagagg
     6541 aattattagc acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggacaat
     6601 atgaaggtca taaaggctat cagttttggc cgaccgatat ttccagcgcc aaagaggtaa
     6661 atcatacggt agcagaaatt atccagcgtt ttggtcgcat cgacggtctg gttaataacg
     6721 ccggggtcaa tttcccacgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac
     6781 tcaacgaagc tgcattcgaa aaaatggtca atatcaacca gaaaggcgtt tttctgatgt
     6841 cgcaggcggt ggcgcgacag atggtcaaac aacacgatgg cgtgattgtg aatgtttcct
     6901 cagaaagtgg gctggaaggc tcagaaggcc agagctgcta cgccgccact aaagctgcgc
     6961 tcaatagctt cactcgttcc tggtcgaaag agctgggtaa gcacggtatc cgtgtggtcg
     7021 gtatcgcgcc ggggattctg gaaaaaacag gactgcgcac gccggaatat gaagaagcgc
     7081 tggcgtggac gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca
     7141 ttcctattgg gcgcgccgga agattagcag aagtcgctga ttttgtttgt tatctgctgt
     7201 ctgaacgcgc cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg
     7261 gataaggagg ctttatggtc aacgccattt tttgtgccca cggcaaatta gcctgcgcca
     7321 tgctggaatc ggttcagatg gtttacggca acgccaatgt tgaagccgtt gaatttgtac
     7381 ccgacgagaa cgccggagac attgtggcaa agctggagaa gttagtgagc attcacaatc
     7441 atgacgagtg gctaatcgcc gtggatttac agtgcggcag cccgtggaat gcggcagcga
     7501 tgcttgcgat gcgcaatcct cgcctgcggg taatcagcgg cctttctctg ccgctggcgc
     7561 tcgaactggt tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc
     7621 aaatcgcaaa gcaatcctgc gtcgtctgga gacaggtggc aacggcagag gaggatttct
     7681 gatgaacatt acgctcgccc gcattgatga ccgcctgatc cacggccagg tcactaccgt
     7741 ctggtctaaa gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga
     7801 tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt
     7861 caatattgaa aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt
     7921 ttatcttttc actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg
     7981 caccctgaat attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt
     8041 ttcattagat gatgatgata ttaacgcgtt tcatgagtta aataatcttg gcgtcatact
     8101 ggatttacgc gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca
     8161 gttaatcgca aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg
     8221 acacttaaaa ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt
     8281 ttttcctgta ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccatta
     8341 attgcctgta cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggt
     8401 ggtacgctgg aattgatagc tctcggctgg atgaacgtcg gcgcggcgca atctccggat
     8461 tctgcactcg ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc
     8521 accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc
     8581 cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag cagcggaaga ggcgcggttt
     8641 cgcactctcg atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgttgctatt
     8701 ccggcactga ttgtctcgct gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc
     8761 attcccgaat ttgtgaccca aggactacag attgctggcg gttttatcgt ggtggtcggt
     8821 tacgccatgg tgctacgtat gatgggtgtg aaatatttga tgcctttctt tttcctcggt
     8881 ttcctcgcag gtggctacct cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc
     8941 atggccctgc tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag
     9001 gccaccacct ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag
     9061 aaaaattaca cgcagcgatc tggtaagcat gtttctgcgc tccaacctgc aacaggcgtc
     9121 ctttaacttt gaacgtattc acgggctggg tttttgctac gacatgatcc ccgccatcaa
     9181 gcgactttac ccattaaaag aggatcaggt tgcggcgctc aggcgacacc tggtgttctt
     9241 caataccacg ccagccgtat gtggcccggt catcggcgtc actgccgcca tggaagaggc
     9301 gcgggccaac ggcgcggaaa ttgatgacgg taccattaac ggcatcaaag tcggtctgat
     9361 gggaccattg gcaggagttg gcgatccact ggtctgggga acgctgcgcc cgattaccgc
     9421 cgcgctcggc gcatctctgg cactttcggg caacattctc ggcccgctgc tgtttttctt
     9481 tattttcaac gcggtgcgtc tggcgatgaa gtggtatggc ctacaactcg gctttcgcaa
     9541 aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc
     9601 gtcgattctc ggactgtttg tgatgggcgt gctggtcacc aaatggacgt caatcaacgt
     9661 accgttggtg gtttcacaaa cgcatgccgc cgatggctcc accgtcacca tgaccgtgca
     9721 gaacattctc gaccaacttt gccctggttt gctggcgctc ggtctgacgc tactgatggt
     9781 tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt ttggcttagg
     9841 gattatcggc aatgcgctgg gcttcctgtc ctgattcttc gccccggcac gactgccggg
     9901 gccatcgctc aacatgaggt ggtttatgaa aacaacagct ctgcgtcttt atggtaaacg
     9961 tgatttacgc ctggaaacct ttgatcttcc tgaaatgcag gaggatgaaa tcctcgcgac
    10021 ggtggtcacc gacagcctgt gcctctcttc ctggaaagag gccaatctgg gtgaaaacca
    10081 taaaaaagta cccgacgatg tggcgaccaa ccccatcatc atcggccacg agttttgcgg
    10141 cgatattctg gccgtgggta aaaagtggca gcacaaattc cagccgggtc agcgttatgt
    10201 gattcaggcc aacctgcaac tccccgaccg cccggactgc cccggctact ccttcccgtg
    10261 ggtaggcggc gaagccacgc atgtggttat tcccaacgag gtcatggaac aagattgcct
    10321 gctggcatac gacggcgaaa cctattttga aggctcgctg gttgaaccgc tttcctgcgt
    10381 gattggcgcg ttcaacgcca actatcatct tcaggaaggt agttataacc acacgatggg
    10441 gattcgcccg caagggcgca cgctgatcct cggcggcacc ggaccaatgg gactgttggc
    10501 gattgattat gcgctacatg gacccgttaa cccgtcgctg ctcgtcatta ccgataccga
    10561 caacgacaaa ttgagttatg cgcgcaagca ctatccatca gaaccgcaaa cactgattca
    10621 ttatctcaat gccgccgatg cagcatttga tacgttaatg gcactgagtg gcggtcacgg
    10681 cttcgatgat attttcgtct ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt
    10741 gctggcgaca gatggttgcc tgaatttctt cgccggaccg caggataaac atttcagcgc
    10801 gccaattaat ttctacgatg tgcattatgc atttacccac tacgtgggca cgtcaggcgg
    10861 caataccgat gacatgcgcg cagcagtcaa attgattgaa gagaaaaaag tgcaggccgc
    10921 aaaagtggta acacatattc ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc
    10981 tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa tacctgccgc ttatgtcact
    11041 cacgcagatt caggatcaag cactggcggc aattctggcg cgtcatcagg ggatctggtc
    11101 cggtgaggcg gagcaatatc tgctcactca tgcagaggca atttcccatg attaatcgcg
    11161 ctacttaact ctgcatgtcg ctggctggtc gccgcggcaa cttcgcgacc ggatgtgcgg
    11221 ctgattgctg aagcagcaca atttgcatga gaaggataaa aaatatcagc gccttaaggg
    11281 gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaatg aagccttatt
    11341 cggcatacat aatctcgaaa attcaatata ttgcagttat ctggtaggac tggtaagcgt
    11401 agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa tacgtgacat
    11461 cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgcctccc gtgatttctt
    11521 actaaccgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc cgggtaaagc
    11581 ctggctggaa catgcactgc cgctaattgc tgaacagttg cagggtcgcc gttcagcggt
    11641 gtttatccct ttcgctggcg taacgcagac ctgggatgat tacacagcga aaacggctgc
    11701 ggttctcgct ccgctgggtg tttctgtcac cggtattcat agcgttgtcg atcccgttgc
    11761 cgcgattgaa aatgctgaga tcgtgattgt cggcggcggg aatactttcc agttgctgaa
    11821 acagtgccgc gagcgcgggc tgctggcacc aattactgac gtggttaaac gtggcgcgct
    11881 gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta ccaccaacga
    11941 tatgccgatt gtcgatccac aaggtttcga tgcgctgaat ctgttcccgt tgcaaatcaa
    12001 cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg aacagcgtat
    12061 tcgcgaactg ctggtcgtcg cgccagaact cacaattatt ggtctaccgg aaggtaactg
    12121 gatcacagtg agtaaaggtc acgcaacgct gggtggcccg aacaccactt atgtgtttaa
    12181 ggctggtgaa gaagcggtgc cgctggaagc gggtcaccgt ttttaagtga attgccggat
    12241 gcatgatccg gcatcttgta catagtgcct gatgcgacgc tttacgcgtc ttatcaggcc
    12301 ttcaggggcg cagtattaat actcgtcccg tccttcatct tcatcaggct gttccagcac
    12361 actgtaagcg actgaacaaa acagcgagtt caggcgctgc atatctccca gtaagcctaa
    12421 atgtagcgaa ctggtttcaa tgctctgcac gttttgctga tgcaggcgat cgacgtgagc
    12481 gtgggaatag cggcgattaa gaatgcgaaa acgatgtttg ctgcggcgca aacgacgggc
    12541 gctggtaaca tcgccagaga agaaaaccga cattgccagt tttaaattac tgagcaattg
    12601 ctcatagagc gcatccagtt ccttcaagcc atcaagcgaa aatgctcgcc ttgcagccag
    12661 cgatttgtcg gcaatttcac tacccatgcg ctcgacgata tcggaggctt gttcaaggtt
    12721 tagcgacatt tcgatgatct ccgcccagcg gcgcgactct tcttctgcca gttcctcttt
    12781 tggcattcgc gccagataaa gtttaatcgc ggtatagagc acgttgatat catccgccag
    12841 cttgcgcagc tctttctcct gccgtggctc gccgtgcatc actttattca acccttccat
    12901 catctgttcc atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag
    12961 cgtgggcgta tccagcgcgc tgacatccag atgtttaggc cgtagctggg tatccagttc
    13021 cggttcatcg cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat
    13081 gaccaggcaa cgtacaaggt tgtagaagac gtggaaatag atcaccagtt ccgcttttgg
    13141 caatgacaac ttccccattg tctctgccag caaatggaca aacggcagga taatcaggct
    13201 acccaccagc ttaaacagca gactacccag cgcgacacgg cgggctgcgg cattggcggc
    13261 actgttgttg agcatcgcca gcaggccgga accgaggtta gcaccaatca ccagacagag
    13321 cgccacgggg aaggagataa tgcctgcagc ggtcagagtc gcggtcagca gtacagcagc
    13381 aaggctggag tagctgataa tggcgaacat cgcgccaatc agcgcatcca gcagaatatc
    13441 gccggtcagc gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc
    13501 ctgcacaatc aactccaacg ccagcaaaat cagcccaaga ccaataccga cgcggcccag
    13561 ttgcccggcg cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa
    13621 tggtgacagc caggataaat cgaaggtgag aatacgcgcc attagcgccg tcccgacatc
    13681 tgcacccagc acaatcacca gagccggtgc gagggctacc agatcctgag cgacgaacga
    13741 ggtcaccagc atggtggtgg cattactgct ctgtaccagt gcggtaacgc cgatccccgc
    13801 gcaaaaggcg agcggctttt tttcgacgct ccggctaagg acagtacgca aacgcgcgcc
    13861 gaagacgcgc attacgccgg ttcgaacaat atgagtcccc cagaccagca gggcgacggc
    13921 agaaagcagg tgaagcagcg ttaacacgag ttcaggttct ccttatcgtt atatgttcct
    13981 gaagcaattg aatgaccaaa ggaccggaaa cacgctgact ggcatcttta ctgtgccttt
    14041 tgaccttcgt ttttccggct aagattattc cagcctgatt ccagtataag tgtttcatat
    14101 taataaagag acagggcact cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt
    14161 tgtgaatcag tccgcgtcat accccagatt cggtgccagc cagcgctcaa catctgagac
    14221 gctcatacct ttacggcggg catagtcttc aacctgatcg cgctggattt gcgccaccgc
    14281 gtaatacttg ctgtccgggt ggctgaagta ccaacccgaa accgatgcac caggccacat
    14341 ggcgaaagat tctgtgagtt tcatgccagt gtgtttttcc acttccagca gctcccagat
    14401 ggtggctttt tccgtatgtt ccgggcaggc cggatagccc ggtgccggac ggatgccctg
    14461 gtagttttcg cggatcagct cttcgttgct gaggttctcg ttcggcgcat agccccagta
    14521 gactttacgc acacgctcat ggagatactc cgcaaaggct tcggctaaac ggtcggcaag
    14581 cgctttcacc atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc
    14641 gtcctcttcc agcccgccag tcacggcaaa tgcgccgatg taatctgctt taccagaaag
    14701 cttcggcgca acgaagtcag cgagacagta gttagcgaag ccggtttttt cggtctgttg
    14761 acgcagatgg tggctgacgt tgatcacatg ggtacgcgtt tcgtcacggt agatttcaat
    14821 gtcatcgccc acacggtttg ccgggaacag gcccaccacg ccacgcggat tcagcgtttt
    14881 ttcggcgctt aatttatcca gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac
    14941 gcccaccact tcatcttcca gaatgcgcgg atacttcccg gccagcgacc aggtcataaa
    15001 gaacggtgtc cagtcgatgt aattacgcag cgtttcgatg ctggcttcga cttcctgcac
    15061 gccgagacgg tgcgccaccg gcggcgtata agcctgccag tcaaaagcga agtcgttatc
    15121 acgcgccgct tccagcgtga ccggtggtgt gcgcggtttc ttgcgcccgt gctgaatacg
    15181 tacggtttcg tactccttgc gggtacgagc gacaaaatca tcacgttgag tatcggaaag
    15241 cagcgccgcc accacgccaa cggtgcgcga tgcgttctgt acatacaccg tcgggccgct
    15301 gtagttctgc tcaattttca ccgccgtgtg cgcttttgag gtggtcgcac cgccaatcag
    15361 taacggaatg gtgaagccct gacgctccat ctctttcgcc acgttaacca tctcgtccag
    15421 cgacggcgtg ataagccccg aaaggccaat caaatcagca ttcacttctt tagcggtacg
    15481 gagaattttt tccgcaggca ccatcacacc cagatcgaca atttcgtagt tgttacattg
    15541 cagcaccacg ccaacgatat ttttaccgat gtcgtggaca tcgcctttca cggtggcgat
    15601 caccatcttg ccgttggttt taccctgctc tttgctggct tcaataaacg gttcgagata
    15661 ggccaccgcc tgtttcatca cgcgcgccga tttgaccacc tgtggcagga acattttgcc
    15721 ttcgccgaac agatcgccga ccacgttcat gccgtccatc aacgggcctt caatcacttc
    15781 aatcgggcgc gtagcctgct ggcgggcttc ttcggtatcc tgctcgataa actcggtaat
    15841 gcctttgacc agcgagtatt ccagacgttt attcacttcc cacgagcgcc actccgcctg
    15901 ctgggcgttg gcggtgtcgt cggttttgct gccgcgatat ttctcggcaa gctccagtaa
    15961 acgctcagtg ccatcgtcgc gacggttaag aatcacatct tccaccgcgt cgcgcagttc
    16021 agcgggcaag tcgtcgtaaa tcgccagctg cccggcgttg acgatcccca tatccatgcc
    16081 attgcgaata gcgtagtaga ggaacaccgc gtgaatggct tcgcgcaccg gatcgttacc
    16141 acggaatgag aaagaaacgt tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg
    16201 tttgatgtct tcacatgcgc cgataaagtc ctgcgcatag ttgttgtgct cttcaatgcc
    16261 ggtggcgacc gcgaagatgt tcgggtcgaa gatgatatct tctggcggga agccaacctc
    16321 ttcggtgagg attttgtacg cccgacggca aatctcgatt ttccgtgcgc gagtatcggc
    16381 ctgtccctgt tcgtcgaagg ccattaccac cactgccgca ccatagcgac gcaacagctt
    16441 cgcgtgatgg ataaaggcgt cgacgccttc tttcatcgaa atagagttaa caatgccttt
    16501 gccctggata cacttcagac ctttttcaat gacatcccat tttgaggaat cgatcataat
    16561 cggcacgcga gcgatatccg gttcaccggc aatcagattg agaaaacgca ccatcgccgc
    16621 ttcggcgtcg agcatccctt catccatatt gatatcgata atctgcgcgc cgttttccac
    16681 ctgctggcgg gccacatcca gtgcctcgct gtatttctct tctttgatca ggcgcttgaa
    16741 cttagcggaa ccggtgacgt tggtgcgttc acccacgttc acaaacaggc tatcttcgcc
    16801 aatgttcagc ggctccaggc cggacaaacg gcaggctacg gggatttccg gcagtttgcg
    16861 cggcgctaat ccttctactg cacgactcat cgcggcaata tgttgtggcg tggtgccaca
    16921 gcagccgccg acgatattga gaaagcccgc ttgcgcccat tcacgtatct gttttgccat
    16981 cgtgtcggcg tcgagatcgt actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc
    17041 tgtgacgtag cattccgcaa tccgtgataa ttcctgcacg tactggcgca gttcgtctgg
    17101 ccccagcgca cagttaaggc cgaaagtcag cgcttcggcg tggcgcagag agttataaaa
    17161 cgcttcggtg gtctgcccgg agagcgtgcg cccggaggcg tcggtgatgg tgccggagat
    17221 cataatcggc agctcaacgc ccagcgcttc aaactccgtt ttcaccgcaa agaccgccgc
    17281 tttggcgtta agggtgtcga aaacggtttc aatcaggatc agatccgcac caccttccac
    17341 cagcgctttg gtggattcgc gataggccgc caccagctgg tcaaaagtga tgttgcggaa
    17401 tgccggatcg ttgacgtccg gagaaataga cgccgtgcgg ttggtcgggc cgagaacacc
    17461 ggcaacgtag cgcggtttct ccggcgtgcg cgcggtccac tcatcagcac aggcgcgcgc
    17521 cagtttcgcc gccgcaaagt tgatttccgc cgacagggat tccatctggt aatccgccat
    17581 cgcaatggtt gtggagttga aggtgttggt ttcgatgata tccgcgcccg cttcaaagta
    17641 ggcgttgtgg atagcggcga tcacttccgg tttactgagt accagcaggt cgttgttgcc
    17701 tttgaggtcg catggccagt cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg
    17761 atagctctgg atcatggtgc ccataccgcc gtccagcacc agaatacgtt catttaactg
    17821 cgcacgcagt tgttccactt tgctgctcac acttgctccc gacacgctca acccagattt
    17881 aataaaaatt caacaaacca tactggcata aacgcatctg tggtaaaagc gaccaccacg
    17941 caacatgaga tttgttcaac attaactcat cggatcagtt cagcaactat tgcattagct
    18001 aacaataaaa atgaaaatga tttccacgat acagaaaaag gagactgtca tggtcgcacc
    18061 cattcccgcg aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt
    18121 tcagtcttta acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagtgt
    18181 ggcactcacg gagctggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct
    18241 aaccacgatg caacagcagg gctttgtgcg tcaggttggc gaacttggac actgggcgat
    18301 cggcgcgcat gcttttatgg tcggtagcag ctttcttcag agtcgtaatc tgttagccat
    18361 tgtccatcct attctgcgca atctgatgga agagtccggc gaaaccgtga atatggcggt
    18421 gctggatcaa agcgatcatg aagcgattat tatcgatcag gtacaatgta ctcacctgat
    18481 gcgtatgtcc gcgcctatcg gcggtaaatt accgatgcat gcttccggtg cgggtaaagc
    18541 ctttttagcc caattaagtg aagagcaggt gacgaagtta ctgcatcgca aaggcttaca
    18601 tgcctatacc cacgcgacgc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac
    18661 gcgcaaacgg ggttattcat ttgatgatga agaacatgcc ctgggactgc gttgtctcgc
    18721 ggcatgtatt ttcgatgagc accgagaacc gtttgccgca atttcaattt ccggaccgat
    18781 ttcacgtatt accgatgacc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa
    18841 ggaagtgacg ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat
    18901 tctgcccgtc atacccgact ggcaatagaa taaggggtct gttaatcgca ttgacgccaa
    18961 aataacttat tgtcatacac ttcacacgct tttattacct cagcattatt tttaaacatc
    19021 aaaccact
//
LOCUS       DARNVM010000090        18897 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.090, whole genome shotgun sequence.
ACCESSION   DARNVM010000090 DARNVM010000000
VERSION     DARNVM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.090"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..800
                     /locus_tag="SI621_002836"
     CDS             <1..800
                     /locus_tag="SI621_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4065527.1"
                     /translation="ITPSAVSQSLQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEK
                     NLRGIEQTINIVNKSELKKNFIIYGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSA
                     ENAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIA
                     EEFTLLISKSAGIDEIQMDIDERFMNRKVSFRSSSLLTIINSISVTDLLGIVPTELYD
                     LHRDFLKLQEIKLEQPLPAVKLYISYNKASLNNLVFSRFIDRLNDSF"
     gene            947..2167
                     /locus_tag="SI621_002837"
                     /pseudo
     CDS             947..2167
                     /locus_tag="SI621_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            2140..2769
                     /locus_tag="SI621_002838"
     CDS             2140..2769
                     /locus_tag="SI621_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEI4065528.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2770..3930)
                     /gene="ybdL"
                     /locus_tag="SI621_002839"
     CDS             complement(2770..3930)
                     /gene="ybdL"
                     /locus_tag="SI621_002839"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HEI4065529.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4039..5127
                     /gene="hcxA"
                     /locus_tag="SI621_002840"
     CDS             4039..5127
                     /gene="hcxA"
                     /locus_tag="SI621_002840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HEI4065530.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSLNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFERPGAKHILFRGHCSESGVQQLAADSGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHFEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAICDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDNVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5137..5334)
                     /locus_tag="SI621_002841"
     CDS             complement(5137..5334)
                     /locus_tag="SI621_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEI4065531.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5462..7567)
                     /gene="cstA"
                     /locus_tag="SI621_002842"
     CDS             complement(5462..7567)
                     /gene="cstA"
                     /locus_tag="SI621_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HEI4065532.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7748..8161)
                     /gene="entH"
                     /locus_tag="SI621_002843"
     CDS             complement(7748..8161)
                     /gene="entH"
                     /locus_tag="SI621_002843"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HEI4065533.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTLDGQCVVGTELNATHHRPVSEGKVRGIC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8164..8910)
                     /gene="entA"
                     /locus_tag="SI621_002844"
     CDS             complement(8164..8910)
                     /gene="entA"
                     /locus_tag="SI621_002844"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HEI4065534.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8910..9767)
                     /gene="entB"
                     /locus_tag="SI621_002845"
     CDS             complement(8910..9767)
                     /gene="entB"
                     /locus_tag="SI621_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HEI4065535.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9781..11391)
                     /gene="entE"
                     /locus_tag="SI621_002846"
     CDS             complement(9781..11391)
                     /gene="entE"
                     /locus_tag="SI621_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HEI4065536.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNRAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11401..12576)
                     /gene="entC"
                     /locus_tag="SI621_002847"
     CDS             complement(11401..12576)
                     /gene="entC"
                     /locus_tag="SI621_002847"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HEI4065537.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAKGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPDQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLHKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSHENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12765..13721
                     /gene="fepB"
                     /locus_tag="SI621_002848"
     CDS             12765..13721
                     /gene="fepB"
                     /locus_tag="SI621_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HEI4065538.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIVQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13725..14975)
                     /gene="entS"
                     /locus_tag="SI621_002849"
     CDS             complement(13725..14975)
                     /gene="entS"
                     /locus_tag="SI621_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HEI4065539.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVICLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15086..16090
                     /gene="fepD"
                     /locus_tag="SI621_002850"
     CDS             15086..16090
                     /gene="fepD"
                     /locus_tag="SI621_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HEI4065540.1"
                     /translation="MSGSVAVTRAIAVPGLLFLLIIATALSLLIGAKSLPASVVLEAI
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     LLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16087..17079
                     /gene="fepG"
                     /locus_tag="SI621_002851"
     CDS             16087..17079
                     /gene="fepG"
                     /locus_tag="SI621_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HEI4065541.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17076..17891
                     /gene="fepC"
                     /locus_tag="SI621_002852"
     CDS             17076..17891
                     /gene="fepC"
                     /locus_tag="SI621_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HEI4065542.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17888..>18897)
                     /gene="fepE"
                     /locus_tag="SI621_002853"
     CDS             complement(17888..>18897)
                     /gene="fepE"
                     /locus_tag="SI621_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HEI4065543.1"
                     /translation="VMAVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTF
                     TKLRVLDLDIKIDRTEAFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRA
                     IVALSEKMKAVDDNASKKKDEPSLYTSWTLSFTAPTNEEAQTVLSGYIDYISALVVKE
                     SIENERNRLELKTQFEKEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNG
                     QAVKDDPDFSISLGADGIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNF
                     TPFKYQLSPSLPVKKDGPGKAIIVILSAMIGGMVACGGVLLRHAMASRKQDAVMEEHL
                     V"
BASE COUNT         4494 a         5326 c         4807 g         4270 t
ORIGIN      
        1 atataacgcc ctctgctgtt agtcagtcgt tacaacgttt acgtgcgcaa tttaatgacc
       61 cgttattcat tcgctcaggg aaaggtatcg cgccgactac gacagggtta aatctgcatc
      121 atcatcttga gaaaaatctc agaggcattg aacaaacgat caatattgtt aataagtctg
      181 agctaaagaa aaattttatt atctacggtc ctcagcttat ttcttgtact aataacaaca
      241 tgctaatccg ctgtcttaga caagatactt caatcgatat agaatgccat gacatactgc
      301 tttcagctga aaatgccgaa gaactgttgg ttcaacgcaa aactgacctg gcaattacac
      361 tacaaccagt aattagccgc tctgtcatct gcatgccgtt acatactatt cgcaattctc
      421 ttatatgtag caacaaacat cctcgtatta ccgataccag taactatgaa caaattatcg
      481 ccgaagaatt caccttgttg atatctaaat cagcagggat tgacgaaatt caaatggaca
      541 tagatgaacg ttttatgaac cgtaaagttt catttcgcag ttcttctttg ttgacgataa
      601 ttaatagtat ttctgttact gacttattgg gcattgtgcc caccgaactg tacgatttac
      661 atcgtgattt tttgaagtta caagagataa aactggaaca accacttcct gcggttaaac
      721 tttatatttc ctataacaag gcttcgttga acaaccttgt cttctcacga ttcatagatc
      781 gtttaaatga tagcttctga tttattacca ctgataaatg ttatattacg catcaattga
      841 ttcattgtgt aaatataaaa taatgtgctc tatcttgctt cgtttaccct ataattagcg
      901 gattcatctc acgagtcacc ccgtaacgtt cagagagcgt tttcagatgt ctatttataa
      961 aatcccgctt ccactgaata ttctggaagc tgcaaaagaa cgtattacct ggacacttaa
     1021 caccctaccc cgcgtctgcg tttctttttc gggtggtaaa gattccggat taatgctgca
     1081 tctgaccgcc gaacttgccc gacaaatggg caaaaaaatt tgtgttttat ttatcgactg
     1141 ggaagctcag ttctcttgca ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga
     1201 tgtcatcgaa gagttttact gggttgcgct cccgcttaca acgcaaaatt ccctatcaca
     1261 ataccaaccc gaatggcagt gctgggaacc tgatgtcgaa tgggtacgtc agcctccgca
     1321 agatgcgata accgatcctg actttttctc cttttaccag ccaggcatga ccttcgaaca
     1381 atttgtgcgt gagtttgccg aatggttttc acaaaaacgt ccggcggcga tgatgatcgg
     1441 catccgtgcg gatgagtcct acaaccgttt tgtcgccatc gccagtttaa ataaacaacg
     1501 ttttgccgat gataagccct ggaccactgc cgcaccaggc ggtcatagct ggtacattta
     1561 ccccatctac gactggaaag tcgctgatat ctggacctga tatgctagcc atcaacgcct
     1621 ctgtaaccca ctgtataata taatgtatca ggcaggcgtt cctctacgtc atatgcgaat
     1681 ttgcgaacct tttggtccgg aacaacggca aggattatgg ctctatcacg ttatcgaacc
     1741 ggatcgctgg gctgctatgt gcgcacgagt cagcggggta aaaagtggcg gcatttacgc
     1801 cggacatgac aaccattttt atggtcatcg gaaaatcctc aagcccgaac atttagactg
     1861 gcaggagtat gcattattgc tactcaacag catgccggag aaaacagctg agcattatcg
     1921 caataaaatc gccatttatt tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac
     1981 ccagcaaggg gacattggcg cgaaagatat cccctcctgg cggcgaatat gcaaagtttt
     2041 actcaataac gattactggt gtcgggcatt atcatttagc ccgaccaaag cgaagaacta
     2101 tcagcgttat aacgaacgaa taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa
     2161 cgattaacgc aggatttaac ccaatttctc gccagcttgc cggaagacga tcgcatcaaa
     2221 gcgattaatg aaatccgcat ggcgatccat caggttagcc cctttcgaga agagcctgtg
     2281 gattgcgttc tgtgggttaa aaacagccaa cttatgccta atgattacaa cccaaacaac
     2341 gtggcaccac ccgaaaaaaa actgctgaaa aaatccattg aaatagatgg ttttacccaa
     2401 cccattgtgg tcacgcatac ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc
     2461 catgaaattg gtaaaggctc atcatcgtta aaattacgct taaaaggtta tttgccggtg
     2521 acctgtctgg aaggtacgcg taatcaacgc atcgccgcga ctattcgtca taatcgcgcc
     2581 cgtggacgtc accaaatcac tgccatgtca gaaatcgtcc gtgaactcag ccagttggga
     2641 tgggacgata ataaaattgg caaagagctg ggaatggaca gcgacgaggt gctgcgcctg
     2701 aaacaaatta atggcctgca agagttgttt gccgaccgtc aatattcccg tgcctggact
     2761 gtcaaataac tacagctggc gcaggcgttc agctgctgcc agcaacgtcg attccttctt
     2821 ggcaaaacag agacgaatca gtttatgggg gaagggatcg gcgcaaaata ccgacagcgg
     2881 gatcgccgct acaccgtgct cccgcgtcag ccactggcaa aactcaacgt catccagcga
     2941 agaaaccgcg ctgtaatcca ccagcaaaaa gtaagtgcct tcgcacggta aaatctccag
     3001 ccgactctca cttaaggcat tcaccagaat atcgcgtttt tggcgataaa agtccggtaa
     3061 cgcaagataa tgctcaggtt ctgcacgtag catatccgca agtgccagct gtgccggggt
     3121 attcaccgaa aaggtcagat actggtgtac cttgcgaatt tcagcgctga tgggcgctgg
     3181 cgcaacgcaa taacccactt tccagccggt catatgatag gtcttgccaa atgaagaaac
     3241 tgccaccgcc cgctcacgca gctgcggatg cgccagcacg ctggcatggc cctgttgtga
     3301 aaagttgatg tgctcgtaaa cttcatcgct aatgacaaaa atctcgtgcc cggcaattgc
     3361 ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt gcactggggt tatgcggggt
     3421 gttgaggatc accagtcggg tgcgctcgct taacaatgcg gcaaattcct gccagtccac
     3481 gcgaaaatgc ggtggttgca gtgcaatacg tttcactatt ccccccgaaa gcgcgatggc
     3541 gggcgcgtaa ctgtcatagc tgggatcaaa gcagatcact tcatcaccat tgcgcaccaa
     3601 tgcggtaatc gctgcatata acgcttccgt cgcccctgcc gttacggtga tatcgctatc
     3661 ggcatccggt tgatagccat acagacgttc cgttttctga gcaatcgcct cgcgcaaggc
     3721 ctgcacgcca gtcataggcg cgtattggtt tgccccctgg gcaacgtggt acgccaggcg
     3781 ctcctgcaaa tagcgcggac catcaaaatc aggaaagcct tgcgacaggt taattgcctg
     3841 gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt
     3901 gctttgtgga atcagagggt tatttgtcat ttttattgta cctgacgaag ttgcgtgttg
     3961 aaggcactat aacacgatgc tagtatttgg caatcaagac gtttagatgt ctaaataaaa
     4021 caataaggac aacacaacat gcctcacaat cctatccgcg tggtcgtcgg cccagctaac
     4081 tacttttcac atccaggcag tctcaatcac ctgcatgatt ttttcactga tgaacagctt
     4141 tctcgcgcgg tgtggatcta cggcgaacgc gccattgcag cggcgcaaac aaaacttccg
     4201 ccagcgtttg aacggccagg ggcaaagcat attttgtttc gcggtcattg cagcgaaagc
     4261 ggtgtacaac aactggcggc tgactccggt gacgatcgca gcgtggtgat tggcgtcggc
     4321 ggcggcgcac tgctcgacac cgcaaaagcc ctcgcccgcc gtctcggtct gccgtttgtt
     4381 gccgttccga cgatagccgc tacctgcgcc gcctggacac cgctctccgt ctggtataac
     4441 gatgccggac aggcgctgca ttttgagatt ttcgacgacg ccaattttat ggtgctggtg
     4501 gaaccagaga ttatcctcaa tgcgccgcaa gaatatctgc tggcggggat cggtgacacg
     4561 ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc
     4621 gtgcggctgg ggatcaataa tgcgcaggcc atttgcgacg tcttgttaaa cagtagcgaa
     4681 caggcgctga gcgatcagca aaaacaacag ttaacgcaat cattttgcga tgtggtggat
     4741 gctattattg ctggtggtgg gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg
     4801 gcggcccatg ccgtgcataa cggtctgacc gtgctgccgc aaaccgagaa gtttcttcac
     4861 ggcaccaaag tcgcctacgg aattctggtg caaagcgcct tgctgggtca ggatgatgtg
     4921 ctggcacaat taaccggggc gtatcagcgt tttcatctgc cgactacgct ggcggagctg
     4981 gaagtggata tcaataatca ggcggagatc gacaacgtga ttgcccacac cttacgtccg
     5041 gtggaatcca ttcattacct gccagtcacg ctgacaccag atacgttgcg tgcagcgttc
     5101 gaaaaagtgg aatcgtttaa agcctgacgt acacatttag cagcagcgcg cgccgccttt
     5161 tccaccgtag cgcgcgtcct gccgctcacg gaaaaactct tcgtaggtca tcggcgtttg
     5221 atcgggatgg ttaacccgca tatgttcgac atagttgtcg taatcaggca taccaatcat
     5281 cagcttcgcc gcctgaccta aatattttcc ggcttttgcc agtgaatcaa acatcaatac
     5341 ctccggtaaa caggttgctg aaatattgcc ggatacggcg cgaacacctt atccggcctg
     5401 gaattgctga gagtaagggg aaccctcggc actatgccaa cattcccccc tcactctgac
     5461 tttagtgcgc gccttttgcc tgcgccacga tctcctcgac attttccggc attggctcat
     5521 acggcgtttc tttcgccgtt ggcttcggct ctttcaatgc cgccagtgcc gtcttaatcg
     5581 agaacagtgc cagaaccacc acgaccacca taaagaagat ggttaacccg gcatcaagac
     5641 ggttgttgaa caccagttgc gccagttgtg actcagtata ctgcgacgga atattgccgc
     5701 tgtcgatcat tgcctggaac ttattagcaa tcgccaggaa gccgactttc gcatccgggc
     5761 taaacgcttt ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca
     5821 gcgccaccca ggcgtaacgt tgacgtttca ttttgaacag caccacagca cagagcatca
     5881 gcgccatccc tgccagcatc tggttggcaa taccaaacag cggccacaag gtgttaatac
     5941 cgcccagcgg atcgaccacg ccctgatgca ggaagtagcc ccacgccagc acgcacagcg
     6001 ccgttgccag caggttagca ggcagtgaat cggtccgttt caggcccgga gacaccacgc
     6061 ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc tgcatccacc gccgtcagaa
     6121 taaacagtgc ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcatgccgc
     6181 ccagcgcacc gtgcagaatg taggccatcc ccaccgccag ggtcggcgca ccgcccgcac
     6241 gggaaatgat cgactgttca cccacttcgc tggcaatctg gtttagcgta tccggggtaa
     6301 tcgcaaagcc ccagctactc accacctgcg cggcagaagc aaccacatcc gccgtccctg
     6361 ccggagccag caccgccatc gggctgttca tggcgaagta cacacccgga tcgatgatac
     6421 aggcggaaac cagcgccata atcgctacga aggattccat taacatcccg ccgtagccga
     6481 taaaacacgc ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg
     6541 catggaagcc agacaccgcg ccacaggcga tggtgataaa caggaacggg aacaggttac
     6601 cggtccatac cgggccagta ccatcaacaa atttggtcag cgcaggcatg gtcagcgtcg
     6661 ggcgcataat caaaatgcct accgccagac cgacgatagt cccgattttc aggaaggttg
     6721 agaggtagtc acgcggagcc agcagcagcc acaccggcag caccgccgcc acaaaaccgt
     6781 aacccaccag catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg
     6841 ggctttctgc cacccagccg ccagagataa tggcgaaaat caggaacacc agaccaatca
     6901 ccgacacttc gccaatacgc cccggacgca aatagcgcag gtagatcccc ataaacagcg
     6961 ccagcggaat ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca
     7021 ccacgatcat cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg
     7081 caatcacccc ggcggttggc cccatctctt ctttgaccag ctcacccagc gagcgaccgt
     7141 cacggcgcgt agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc
     7201 cggcgagcag ccagatcatc cccggcaggt agcccatttg cgccgccagc accggcccaa
     7261 ccagcggacc tgctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg
     7321 tcggcacata gtccagcccg tcgttatggc gcacagctgg cgtcatacgc gtcgggtcaa
     7381 ccgccagcac atttttggcg atatacaggc cataaaaacg gtaagcgatc agatagatac
     7441 agaccgacgc caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt
     7501 atcccagagc aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt
     7561 tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca
     7621 tctttgtaca atttaacaac acagcaacca tgtaaatcgg agttgaaacc aatatttaac
     7681 ttaacaatgt taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct
     7741 gtcgggctca tcccaaaact gccgtaccca gccgacaggt gcagcaacgc cgcccctgtt
     7801 catcgaaaac aacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga
     7861 tgccgcgtac cttgccttca gacaccgggc gatgatgtgt ggcgttaagt tccgtgccca
     7921 ccacacactg tccgtcgagg gtcatcataa atccggccat cgatcccagc gtttccgcca
     7981 gcgccgccga cgcgccgcca tgtagtaaac cgaacggctg atgagtacgg gtatcgaccg
     8041 gcatttcggc ttcgagtaca tcatcgccca gacgggtata cacaattccc agatgcgcca
     8101 ccattgtgtt atcgctggtg gcgttcagct cgtcgagcgt taaatggcgt ttccagatca
     8161 tgcttatgcc cccagcgtcg agccgccatc taccacaata tcctgtagcg tgatatggct
     8221 ggcgaggtca gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt
     8281 ccccagcgga atgccgagtt taaactgctc gccaaagccg cgaatacgct gctcttcggc
     8341 gtcatcgctc acccacagcg tgcgttgcat atcggtgtcg gtggagcctg gcgaaaccac
     8401 attacagcgc acgccgctac ccgccagttc cagcccaacg ctcaacgcca ggcttttcag
     8461 cgccgctttc gacgcgccat aagcactcat accaatgcgc ggcgtgtgtg cggcgtcgga
     8521 cgcaacagta acaatcgccc cgccccgctg acggcgaaac tggttcatgg tctgctggaa
     8581 caggttaaac gcaccgccga cattgaccgc aaaagtctgc tgccagtcct ccttactgag
     8641 ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc
     8701 cgtttcagct aacagtcgct gacatacctg cgccacctgc gcagcatcgg caacatccat
     8761 tacttcggtc gcaaaaggat attgctcctg agtaaacgct tgatcaaaac ctgtaacttt
     8821 cgcccccgcc tcaacaaatg ccagcgccgt agcatagccg atacctttcc ccgcgccagt
     8881 tacccagaca tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac
     8941 caggcgtcga tggtcgggtt tttcgccagc atgacaaagt cgatatcgcc atgcactttg
     9001 cgccagcgcg ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg
     9061 tcgtcatcga acggttcatc ggactcgtcc agcaacggca ggatcacctc acgcagcgcc
     9121 gctttgctgg cggggatagg tgctggcagt aattcttcgg tcatcaccac ccggccagaa
     9181 cgtccggcca catatttcag cgacatcaaa tgctcgtcac ggctgaaatc agccagcgca
     9241 tccgccacca taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag
     9301 ccaatgtggg catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt
     9361 tgctccagcg gggaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg
     9421 gcatctggcg tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggccaggc
     9481 ccccacatat cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg
     9541 taataaaccg ggatattgtg ctgtttgcag tagtcgcgta gtgccgcaat attcgcgatc
     9601 acctgctcca tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca
     9661 tggattaaca acgcggcacg ttgcggttca aaggcccagt caactttatt ctgcggaata
     9721 tcgtgagact ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct
     9781 tcaggctgat gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac
     9841 cgccgtcagc ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa attcggcaat
     9901 accctgttca cgcaggaaac gacgcacctg caccgcgcgt agaggctctt ttaccaccag
     9961 ataagcacag cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcatagat
    10021 caccgccgga tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc
    10081 acggttgatc tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga
    10141 gatcagatcg ccggaacagt aaaaaccgtt ggcatcaaag gcactggcat tgtgctgcgg
    10201 gcttttgtaa taaccgcgga aggtgtacgg cccgcgcgtc atcaggcgtc caacttcccc
    10261 ttgcggcagt ggatttcctt cggcatcggc aacccacact tcatcgtccg ggcacattgg
    10321 gtaaccctgg gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag
    10381 cccttccgcc atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc
    10441 cgcaagcgtg gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg
    10501 cgcccggctt tcgccttcgg tcagcgcctg taaccacagg ctgactgcgg gtggcaccag
    10561 cgcggtgacg ttcacctgat gtttttcaat caatgggaaa cagagcgtgg cgctgggatc
    10621 ggccgccaga acgaccgttc ctccggcaag aaagacgccc agcgatcccg gcgaactcat
    10681 ggcgtagtta tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca
    10741 aatctcgacg ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt
    10801 cggtgtgccg gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgacgg
    10861 cgtggcagta aaatcctcag ccggatggtt aatcgcatcc tgcaaattat gctcaccgct
    10921 gtcgttgtgc agttgcacca cgcgaatgga ggaatgctct gcaacaaatg tattgaggaa
    10981 atcatccccg ctaaacagcg catgctggcg atcggcaatc agtaacgccg gttcaatctg
    11041 gctggcatag gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac
    11101 gcccagcttc agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagctgtac
    11161 cagcgcggtt tcaccaggtt taatgccctg gcggcgcaaa ctacaggcga ggttatctgc
    11221 cgcccgattc agctcccggt aactcaactg ccgctcgccg tcgatgaccg cgatgctgtc
    11281 gcttgcggca tgacgggtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc
    11341 ccgatagcga cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcactcc
    11401 ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg acgcccgttt cgcgccactc
    11461 acccaacggt gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc
    11521 ccgcagcttc gcgcagcgga tggtcaccac ccactcgccg ttaccttcgc tgtcacacca
    11581 acccacaatg ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgggt
    11641 cgcggcctga tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagcgt
    11701 cagtgcgttc tcatgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt
    11761 cggcgtggtg atcagttgtg gagaagaagg gacgtgtaac tcactacttc gttcgcgcag
    11821 tacctctttc atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag
    11881 cagacgatta cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc
    11941 taacggaatg gagctaaaac gctcgccgtc tttatgtagc agcagttccg ggctggcccc
    12001 cagcagaacg ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc
    12061 aatcaaccgt tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa
    12121 ccgtgacaac accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgagcaac
    12181 catctgttca aacgtggttt gatccggaat tgcctgacgt tccaccacgt tcagcgactg
    12241 gctgcgggtg aaacggcgtg cggaagcttg tttttcctgg cgggagaacg actgccagga
    12301 ttcaggaata tacagcgatg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac
    12361 cggatttttg atgcccttcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa
    12421 gggactgtcg ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga
    12481 cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat
    12541 ggtctgctgt acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag
    12601 gctttaataa tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta
    12661 tgtcaactct tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat
    12721 gaaaatgaga agcattaact tcattaataa caggaagctg acttgtgaga ctcgccccgc
    12781 tctaccgcaa cgcccttcta ttaacaggac ttttgctttc aggaatagtc gcagttcagg
    12841 ccgccgactg gccgcgtcag attactgaca gccggggcac tcataccctg gaaagccagc
    12901 cgcagcgtat tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc
    12961 cggtgattgc cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt
    13021 tacgccagtg gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac
    13081 cgagcgccga agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaactggcg
    13141 gggattcggc gctggcgctg tacgatcagc tttctaccat cgccccaaca ttaatcatca
    13201 attacgacga caaaagctgg caggcgctgt taacgcaact tggcgaaatt accgggcatg
    13261 agaaacaagc ggcagagcgg attgcacagt ttgataagca actggcggcg gcgaaagagc
    13321 aaatcaaatt accgccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg
    13381 ccaatctctg gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgt
    13441 tggcgaaatt gcccgcaggg ttaaacgcca gccaaagcca gggcaaacgt catgatatcg
    13501 ttcagcttgg tggggaaaat ctggctgcag ggttaaatgg cgagtcacta ttcctgttcg
    13561 ccggtgatca gaaagatgcc gatgctattt atgctaatcc gctgctcgcg cacctgcctg
    13621 cagtacaaaa caagcaggtt tatgcgctgg gaaccgagac gttccgactg gattactaca
    13681 gcgccatgca agtgctggaa cgacttaagg cgctgtttta agcattaact gtcggacgct
    13741 gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caataacaac
    13801 acaccgataa tcaacaaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc
    13861 gctcccagac cgcctaacag cgccgcacct atggcatcgc ccgtcacgtt ttgcgccgtc
    13921 cacaaaccgt taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtgtat
    13981 tgcagcaacg agctgaccgc gctcagccag ccgaatagcg ccagacaaat cacgcctaaa
    14041 atccacatcg gcatcaggcc aaacagccca atggcgagga acgatcccag cgtggagagc
    14101 agcatcaata accctggtcg cgcgctatgt gccagcttcc cgctggttaa cgcgccaata
    14161 gccgcgccga gcgggatcgc cgcatagaga aaaccaatct gtgccgccga catctgccag
    14221 ttgtcagcca gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc
    14281 agcagcgcaa tccctcccac cagcgggctg gcgagcagaa aacgaaatcc tgccagtaat
    14341 gatttcaacg gatgctcacg cggctgaggt ggcggtggca acgccggaag gcttaataac
    14401 ggcagcaagg taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccgccg
    14461 gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg accccaggcg cacggtcaac
    14521 atggtgatcg ccccggcctg cattaagttt tcacgcccta ccagtgcagg tgtcgccgcc
    14581 agtagcgccg taacgccaag tgatgcgaaa aaaccatccc ataaaccaag taaatagatt
    14641 gccaacaatg acggctccgg cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca
    14701 caggtgccgc gcgccagcaa aatcactttt ttgcgttcat agcgatccgc cagcacaccg
    14761 ccgaccatca ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc
    14821 caggtagaat gcgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga
    14881 gacacaattg agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac
    14941 aggctgaggt taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt
    15001 taaataccta ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat
    15061 taatttcatt atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc
    15121 cgtgcccgga ttgctgttct tactgattat cgcgacggca ttaagcctgc tcattggggc
    15181 aaaatcactc cccgcttccg tagtgctgga ggccatctcc ggcacctgcc agagcgccga
    15241 ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt gccggtttac tggccggcgg
    15301 tgcgcttggg cttgcagggg cgctgatgca aaccctcacc cgaaacccac ttgccgaccc
    15361 cggcttgctt ggcgtgaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt
    15421 tggttactct tccgcacagg aacaactggc gatggccttc gccggggcgc tggtggcctc
    15481 attgattgtt gcctttaccg gcagccaggg cggcgggcag ttaagtccgg tgcgtttaac
    15541 cctggcgggc gtggcactgg cggcgttgct ggaagggctg accagcggca tcgccctgct
    15601 taatcctgac gtctacgatc agttgcgttt ctggcaagcc ggttcgctgg atattcgcaa
    15661 tctacatacc ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt
    15721 actgagccgc gcgctgaaca gtttaagcct cggcagtgac accgcgacgg cgctgggtag
    15781 tcgcgtggcg cgcacacagt tgattggtct gctggcgatt accgtgcttt gtggtagtgc
    15841 gacggcggta gttggcccga ttgcctttat tggcctgatg atgccgcata tggcacgttg
    15901 gttggtgggt gccgatcatc gctggtcgct gcccgtcacg ctacttgcca cccctgccct
    15961 gctgctgttt gccgatatca tcgggcgggt gattgtgccc ggcgagttgc gcgtttctgt
    16021 ggtcagtgcc tttattggcg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg
    16081 tggcgcatga tttacgtctc tcgccgatta atcatcacct gtttgctgct aattaccgcc
    16141 tgtgtggttg caggtatctg gggattacgc agcggtaccg tcacgctgga aacctcgcag
    16201 gtattcgccg cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg
    16261 cgtttaccac gcgtgctgat ggcgctgtta attggcgcgg ctctgggcgt cagcggcgcg
    16321 atttttcagt cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc
    16381 ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatc
    16441 gccctggcag caatggcggg cggcattgtc acctcgctgc tggtctggct gctcgcctgg
    16501 cgtaacggca tcgacacctt tcggttgatt attatcggca tcggcgttcg cgccatgctg
    16561 gtggccttta atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcaggt
    16621 ttgtggaatg ccggatcgct caacggcctg acgtgggcaa aaacctcgcc ctccgcaccc
    16681 atcattatat tgatgctcat tgccgccgcc ttgctggtgc ggcggatgcg cttgctggag
    16741 atgggtgatg ataccgcatg tgcgctgggc gtcagcgtcg aacgttcgcg gctgttaatg
    16801 atgctggtcg ccgtagtact taccgcagct gccactgccc tggcggggcc gatttccttt
    16861 attgctttag tcgcgccaca cattgcccgc cgcattagcg gcaccgctcg ctgggggctg
    16921 acccaggcgg cgctgtgcgg ggcgttgtta ctgctggcgg ccgacctctg cgcccaacaa
    16981 ctatttatgc cgtatcaact tccggttggc gtcgttaccg tcagcctcgg tggtatttac
    17041 cttatcgtct tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc
    17101 gtggcgaaca gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag
    17161 aaattcctga tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt
    17221 tactgcgtac tttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggtg
    17281 agcacattca acattacgcc agtaaagagg ttgcacgccg gattggcctg ttggcgcaaa
    17341 atgctaccac gccgggcgat atcaccgtgc aggagctggt ggcgcgtgga cgttacccgc
    17401 atcaaccgct gtttacccgc tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc
    17461 aggccacggg aatcactcat ctggcagatc aaagcgtgga taccctttct ggcggacaac
    17521 gccagcgagc gtggatagca atggtgctgg cccaggaaac ggcaattatg ctgctcgatg
    17581 aaccaacgac ctggctggat atcagtcatc agattgatct cctggaactg ttaagcgaac
    17641 tgaaccgcga gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc
    17701 gttacgccag ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga
    17761 aagagattgt cactgctgaa ttgatcgagc gcatttatgg tctgcgctgc atgataattg
    17821 acgatccggt agccggaact ccgctcgtgg tgccgctcgg acgaacggca ccttcaactg
    17881 ccaagattta aactaagtgc tcttccatca cggcatcctg ttttctggat gccatcgcat
    17941 ggcgcaacag cacgccacca caagccacca tcccgccgat catcgcagaa aggatcacaa
    18001 taatcgcctt acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa
    18061 acggcgtaaa attcacatcg ttgatatttg cttttgttag ctgctcaaca agatactggc
    18121 gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac cgctttttca atttccagtt
    18181 tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc ggggtcgtct ttaacagcct
    18241 gaccattact gtaaacgggc tttttaattc ctgccgcgtt agcaatgtcg agtgaataat
    18301 tgaggcgctg aatgtttgca tcaagttgat ttttcatttt aatgcggtcc tgagccagtt
    18361 tttctttttc aaactgggtt ttaagctcca gtctattgcg ctcattttct atcgactctt
    18421 tcaccaccag agcagagata taatcgatat acccgctcaa aacggtttgc gcctcttcat
    18481 tggttggcgc ggtaaaactt agcgtccagg aggtataaag tgacggttca tcttttttct
    18541 tactggcatt gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca
    18601 aatccagttc gtcgattttc gcctctttta attggtccat cacataaggt gttgagcgca
    18661 ggtactcttc cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg
    18721 tacgatcaat tttgatatcc agatccagca ctcgaagctt agtgaatgtt ttctccagct
    18781 cttgccactg aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca
    18841 ggatgaaaga gatcagcaag cctgcgcagg caaacgcaaa aatgaccgcc atgaccg
//
LOCUS       DARNVM010000091        18870 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.091, whole genome shotgun sequence.
ACCESSION   DARNVM010000091 DARNVM010000000
VERSION     DARNVM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.091"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(210..551)
                     /locus_tag="SI621_002854"
     CDS             complement(210..551)
                     /locus_tag="SI621_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HEI4065544.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(564..1436)
                     /gene="copD"
                     /locus_tag="SI621_002855"
     CDS             complement(564..1436)
                     /gene="copD"
                     /locus_tag="SI621_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEI4065545.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLMFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1440..1814)
                     /gene="yobA"
                     /locus_tag="SI621_002856"
     CDS             complement(1440..1814)
                     /gene="yobA"
                     /locus_tag="SI621_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HEI4065546.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1953..2183
                     /gene="holE"
                     /locus_tag="SI621_002857"
     CDS             1953..2183
                     /gene="holE"
                     /locus_tag="SI621_002857"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HEI4065547.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2285..2941
                     /locus_tag="SI621_002858"
     CDS             2285..2941
                     /locus_tag="SI621_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEI4065548.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            2965..3627
                     /gene="exoX"
                     /locus_tag="SI621_002859"
     CDS             2965..3627
                     /gene="exoX"
                     /locus_tag="SI621_002859"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HEI4065549.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3624..5684)
                     /gene="ptrB"
                     /locus_tag="SI621_002860"
     CDS             complement(3624..5684)
                     /gene="ptrB"
                     /locus_tag="SI621_002860"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HEI4065550.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(5841..6266)
                     /gene="tnpA"
                     /locus_tag="SI621_002861"
     CDS             complement(5841..6266)
                     /gene="tnpA"
                     /locus_tag="SI621_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc46 family
                     transposase"
                     /protein_id="HEI4065551.1"
                     /translation="MSNHDDLLAGFLRKRHSVSKLVVHLIFTTKYRRKLFDSQMIAQL
                     REAFGSAAAKLECEIIEMDGEPDHVHLLVAYPPKLAVSVMVNNLKSVSSRLLRQQNAH
                     LRMQSKTGLLWSRSYFVCSTGEATIETLRAYVQSQSTPD"
     gene            6323..7492
                     /locus_tag="SI621_002862"
     CDS             6323..7492
                     /locus_tag="SI621_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEI4065552.1"
                     /translation="MLRATKVRIYPTPEQAEFINAQFGAVRFAYNKALHIKKHSYQRH
                     RVNLNPRKDLKPLLAVAKKSRRYGWLKEYDSIALQQAVINLDVAFSNFFNPKLKAGKP
                     TFKSKHGRQSSYHCVGIKVLDAAIKIPKILPIEARLHREITGMLKSITLSRSATGKYY
                     ASLLCDDGMEAPAKPALISNVTGLDMGLAHFVIKSDGNKIASPRHLINASRNLRRKQK
                     ALSRKKKGSANRRKARIQLAAVHERVAGARADFQHKLSRAIVDENQAVIVETLKMANM
                     MKNHHLARAIGDAGWSGFITKLEYKATEKGVHLVKLDQWFASSKTCHCCGHKMSEMPL
                     HKRIWQCPECGVEHDRDINAALNIKRKGILELQAAGLVVSAHGGQRKSVTQTVAA"
     gene            complement(7656..8315)
                     /locus_tag="SI621_002863"
     CDS             complement(7656..8315)
                     /locus_tag="SI621_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEI4065553.1"
                     /translation="MANWLNQLQSLLGQSSSSASSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8642..8998)
                     /locus_tag="SI621_002864"
     CDS             complement(8642..8998)
                     /locus_tag="SI621_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HEI4065554.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(9065..9355)
                     /gene="yebG"
                     /locus_tag="SI621_002865"
     CDS             complement(9065..9355)
                     /gene="yebG"
                     /locus_tag="SI621_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HEI4065555.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9489..10667
                     /gene="purT"
                     /locus_tag="SI621_002866"
     CDS             9489..10667
                     /gene="purT"
                     /locus_tag="SI621_002866"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEI4065556.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFRKAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSEN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            complement(10722..11363)
                     /gene="kdgA"
                     /locus_tag="SI621_002867"
                     /gene_synonym="eda"
     CDS             complement(10722..11363)
                     /gene="kdgA"
                     /locus_tag="SI621_002867"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEI4065557.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11400..13211)
                     /gene="edd"
                     /locus_tag="SI621_002868"
     CDS             complement(11400..13211)
                     /gene="edd"
                     /locus_tag="SI621_002868"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEI4065558.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13446..14921)
                     /gene="zwf"
                     /locus_tag="SI621_002869"
     CDS             complement(13446..14921)
                     /gene="zwf"
                     /locus_tag="SI621_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEI4065559.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15259..16128
                     /gene="hexR"
                     /locus_tag="SI621_002870"
                     /gene_synonym="yebK"
     CDS             15259..16128
                     /gene="hexR"
                     /locus_tag="SI621_002870"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEI4065560.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16256..17698
                     /gene="pyk"
                     /locus_tag="SI621_002871"
     CDS             16256..17698
                     /gene="pyk"
                     /locus_tag="SI621_002871"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEI4065561.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17830..18801)
                     /gene="lpxM"
                     /locus_tag="SI621_002872"
                     /gene_synonym="msbB"
     CDS             complement(17830..18801)
                     /gene="lpxM"
                     /locus_tag="SI621_002872"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HEI4065562.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         4710 a         4865 c         4652 g         4643 t
ORIGIN      
        1 gagagagccg tgcgctaaaa gttggcatta atgcaggctt agttgccttg ccctttaaga
       61 atagatgacg acgccaggtt ttccagtttg cgtgcaaaat ggtcaataaa aagcgcggtg
      121 gccatcagct taaatgttaa aaaccgcccg ttctggtgaa agaactgagg cggttttttt
      181 attggaaatc aaaaggctat tttaggtaat taacagagtt tttcagctcg ttctataaac
      241 ggtgccagac tcattttttc gccgggattg ttaggatcat caatctgaat caccgaaatg
      301 ggttgggctt tagtcttccc actggcaact tccttttgtg cgatatcgtt taaaggatac
      361 tgcacgaggg tacttgggtt gatgacatac aaagcattac ccggtcggca agtcagcatc
      421 acctcttcgc gattaaacgc ccatttgtct ttacccactt caaaacggct aacggtaatc
      481 acctgcggtg cagccagcgc cgctgcagaa ctggtgagta acagaaacgc cagaatactt
      541 tttttcatca ttttcgtaat ccatcaaaaa ggttcgagag tcgcaagcag gctgatgatc
      601 accagcacta ccgcacctat cgcccattcg agtttagtca tccacacaaa ccagggtgcc
      661 gcacgatctt catcctgcct catgcgcggt acaagaacat aacgattagc cagcgcgatg
      721 accaccataa tcatcaccag gatggctttg agcaataaga gttggcccca gtaagtcgta
      781 agcgtggggg gaaatccggt aatcaacaat gcattaagca cgccgcttgc cagtacgccg
      841 atcaccgcaa aatgcccgca ccaggaaaaa cgcatcagcg cctgaatagc ctggtgtcgc
      901 caacgacctt tgatgagttg catacaccag agcaccggca gtaatccccc aaaccacgcc
      961 gccgcacaga tcaggtgaat cgcgtgatta gtctgatgga ttttcgccgt taccccttca
     1021 ttcagcgtcg catgcccgac tcccgccagc agaataaatt gcgcggtagt gagcataaac
     1081 atcaaacgtg gcatattccg cggttgcata agggcgacga tcagcgttac gagggcgaga
     1141 acaatttgcc ataaccagac gccaccaaac tgcgtttgca aaacagcctg ccaaatgttt
     1201 ggcgaaaata catctgtcca tcccgttccc atcaacccgc cctgaacggc gagcattgca
     1261 gtggcactga tcaaactcca gacggcagcg tgctgttgta aacgcagaaa acgcttcgtc
     1321 agcaagcgac gaatcgttaa gggggccagc caggcaccgt acattgcgaa gccaaagacc
     1381 agcatcagcg aggtgaaatg gataaatcgc agcgcgatcc aggtaaacgc cagcatgatt
     1441 tatttcacgc taaaggtgta gtgtcctttc gttttatgcc cgtccaccga aacaacatgc
     1501 cagtcaacgg tgtaggttcc gggtttcagc gaatcggcca gtggaacaat cagttgcttt
     1561 tggtcctgct cattccgctt cgccggtaat gttttaatat tttcgttttt tggccccgtg
     1621 atttttgcac cactgaatcc tgtttcaacg ccttccgaga agtttaaagt gattgcctgc
     1681 ggggcagctg tcacttgcgc atttgccgca ggatactgat gcgttaaatg cgcatgtgcc
     1741 cagacggaag gtgtcaccaa cgaagtggtc aggattgcca gcgcgtagcg aagggagcgt
     1801 gcagttgaag ccatattatc tattcctttt tgtaataact tttttacaga gcataacctt
     1861 gtctaatgtc tgagtcgagg atcatcaatt ccggcttgcc atccaggctc actcttagta
     1921 acttttgccc gcgaatgatg aggagattaa gaatgctgaa gaatctggct aaactggatc
     1981 aaacagaaat ggataaagtg aatgtcgatt tggcggcggc cggggtggca tttaaagaac
     2041 gctacaatat gccggtgatc gctgaagcgg ttgaacgtga acagcctgaa catttgcgca
     2101 gctggtttcg cgagcggctt atcgcccacc gtttggcttc ggtcaatctg tcacgtttac
     2161 cttacgagcc caaacttaaa taaaacttat acagagttac actttcttac ataacgcctg
     2221 ctaaattatg agtattttct aaaccgcacc cataatttgc agtcattttg aaaaggaagt
     2281 cattatgtcg ttctggaaag ttgcagcagc gcaatatgag cctcgtaaaa cctcactgac
     2341 cgaacaggta gctcatcatc tggagtttgt gagggccgcc gccaggcaac aatgtcagct
     2401 gttagttttt ccatcgcttt ctttactggg gtgtgattat tcgcgacgtg cccttcctgc
     2461 cccacccgat ctctcgctgt tagacccgct ttgttatgct gcaacgacct ggcgaatgac
     2521 catcattgcc ggccttcccg ttgaatataa cgatcgcttt attcgtggaa ttgcggtgtt
     2581 cgcaccgtgg cgaaaaacac cagggatcta ccatcagagt catggtgcat gtctgggccg
     2641 tcgttccaga accatcacgg tggtcgatga acaaccggaa ggtatggata tggatccgac
     2701 ctgttcactg tttacgacag ggcaatgtct gggggaaccc gacctactgg catctacccg
     2761 ccgcttacaa tttttttcac atcagtactc aattgccgtg ctgatggcaa atgcccgtgg
     2821 taacagtgca ctatgggatg aacatggtcg tctcatcgtt cgcgccgatc gtggttcatt
     2881 attgttagtc ggtcagcgtt catcacaggg ttggcaaggc gatatcattc cattacgcta
     2941 ggctttttcg gcctggagca tgccatgttg cgcattatcg atacagaaac ctgcggtttg
     3001 cagggaggga tcgttgagat tgcctctgtt gatgtcattg acggaaaaat cgtcaacccc
     3061 atgagccacc tggtgcgccc cgatcgtcct attagtccac aggcgatggc gattcatcgc
     3121 atcaccgaag ccatggtcgc cgataaaccg tggattgaag atgtgatccc gcactattac
     3181 ggtagtgaat ggtatgtcgc gcacaacgcc agctttgacc gccgcgtact gcctgagatg
     3241 cccggtgagt ggatttgcac tatgaaactg gcccgtcgtt tgtggccagg gatcaagtac
     3301 agcaatatgg cgttatataa aacacggaag ctcaatgtac agacgccgcc gggcctgcat
     3361 catcaccgcg cgttgtatga ctgttatatc accgctgcgt tgcttatcga tattatgaac
     3421 acctccggct ggacggcaga acagatggcc gatatcaccg gacgtccgtc gttgatgacg
     3481 accttcacct ttggcaaata ccgtggcaaa gcggtttccg acgttgccga acgcgatccg
     3541 ggctatctgc gctggttgtt taataacctg gacagcatga gcccggagct gcgtttaaca
     3601 ctgaaacatt atctggaaaa tacttaatcc tcaggcgtag cgggtaatgt tccctgcgcc
     3661 agcgcgacca gaaaagcata ttccatcgct acgccttcgt acgatttaaa gcggccagat
     3721 ttaccgccat ggcctgagtc catgtcggta cagagcaata aaagatggtt atcggttttc
     3781 agctcgcgca atttagcgac ccattttgcc ggttcccaat attgcacctg agaatcgtgt
     3841 aaaccggtcg ttaccagtaa atgcggataa gcctgtgcgg tgacgttgtc atacgggctg
     3901 tagcttttca tgtactcgta atattgcgga tcctgcggat tcccccactc ttcaaactca
     3961 ccagtggtaa gaggaattga ttcatcaagc attgttgtta caacatcaac aaacggtacc
     4021 tgggcgataa cgccgtggaa caattccggg cgttgattaa ttgcaacgcc catcaacatg
     4081 ccccccgcac tcccgcccat cgcataacag agcgaaggag agccatagcc cagttttaac
     4141 aatgcatcgc aggcatcaag ataatcatta aacgtatttt tcttcttcag gaattttccg
     4201 tcttcgtacc attgttgccc cagctcacca ccgccgcgaa catggacaat ggcgtagaca
     4261 aagccacgat ctaacaaact caagcggcta aaactgaaat cggcatcaat acttgcgccg
     4321 taagaaccat agccatacac cagcaatggg ttgtgtcctt tgcgaaaatg tttgcgatga
     4381 tagaccagcg aaaccggaac ttcgacgcca tcacgggcga ctatccacag gtgttcactg
     4441 cggtaattcg ccgcatcaaa accgggcact tctgtttgtt ttaatacacg acgctcaccg
     4501 gtatccatat ccagttcaaa caaagtgtct ggtgtggtca tggaagaata accataacgc
     4561 aatcgcgcgg tttcaggttc tggattgtag gcaatccagg tcacataggc cggatcatca
     4621 aaggcaatgc caatgacttc ccgggtcttg cggttgattt ggcgcaaact ggttaacccg
     4681 cgctgacgct cttcaaccac cagccagtcg gtaaacagcg taaacccttc cagcataatg
     4741 ttatcgcgtg gcggaattaa ctcttcccac tgttgctcat cacgcatacg ggtacggtat
     4801 aagccaaagt ttttgccgtg gcggttggaa cgcagataaa accgatgctg gtagtgatca
     4861 aggctgtatt cgtgatcttt gcggcgcggc agaaaaacaa acggctcggc atcggccatt
     4921 tccgcgtcca gcaggcgaac ttcactggtg gtggcgctgg ccagatgaat gactacatag
     4981 tgcttcgacg tcgttttatg caggctgaca taataggtat cgtctttttc ttcgtagatc
     5041 agtgtatctt gcgatgctgg cgtaccgatg gcgtgacgcc agacctgata aggaagcagc
     5101 gtcaccggat gcttgcgaac atagtagaaa gtccaggaat catttgccca gacaaagctg
     5161 ggttcaacgt tatccagcag ttccggatac cagttacctg tttccagatt acgaaaacga
     5221 atgccgtact ggcgtcggga aagaaaatct tctgccagcg ccataatggt gttatcgggc
     5281 gtaatcgcca ttccgcccat cgaataaaac tcactatgag ctgcgcgctt attggcatcg
     5341 agcaatattt cccactcatc ccactcttca ctgaatgccg attgacgctg gtagatagca
     5401 tattcacagc ctggttcata aatatgccga tagcggtagc cattttttat gtagggcgca
     5461 gaaacttctc tttgcggaat gcggtcgatg atttccttta agatgcgatc ctgcaaggct
     5521 tgttgcgagg ccatcacccg atgaccgtaa ctattttctt gttgcaggta gtccaggact
     5581 tccggctgag aacgcgtatc gtcccgcagc cagtagtaat tatcgatgcg cgtatcgcca
     5641 tgaagcgtca tggcgtgggg aatgcgggcg gcttttggta gcatgttatt gttctttctg
     5701 gttgaaacat cttataaggg tggcaaaact caccggagat gcaagcgaaa cagggtagtc
     5761 attgcttagc gaaaaacgcc gtaaaccctc gcccaatgcg ggcggggata taaggcgaaa
     5821 agcccgcagg gctttaaaga tcaatcaggt gttgactgac tctgaacgta ggctctgagc
     5881 gtttcaatcg ttgcctctcc ggtgctacag acaaagtacg atctcgacca caaaagcccc
     5941 gttttactct gcatgcgtaa atgcgcgttc tgctggcgta gcaaacgtga tgataccgat
     6001 tttaaattat tgaccatcac actgaccgcc agttttggcg ggtaggcaac cagcaggtgt
     6061 acatggtctg gctccccgtc catctcaata atttcgcatt caagctttgc cgcagcggag
     6121 ccaaaagcct cacgcaactg agcgatcatc tgactgtcga atagtttgcg tcgatacttt
     6181 gtcgtaaaaa taaggtgcac aaccagctta ctgacactgt gtcgtttgcg gagaaatccc
     6241 gccagtaaat catcatgatt actcaattga tatatttacc taaagtgtta aaataaaagc
     6301 attatatcaa tgagcgctga ttatgttaag agcaacaaaa gtacgcatct acccgacacc
     6361 agaacaggct gaatttatca acgcccagtt cggtgcagtg cgttttgcgt acaacaaagc
     6421 tctgcatatc aagaaacatt cttaccagcg gcaccgcgta aatttaaacc cgcgtaaaga
     6481 cctcaaacca ctgcttgcag tggcgaagaa atcccgccgg tatggatggc ttaaagaata
     6541 tgattctatt gcgttgcagc aggcggtaat taacctcgac gtggcttttt ctaacttttt
     6601 caatccgaaa ctgaaagcag gtaaacctac ctttaaaagt aaacacggca gacaatcgag
     6661 ctaccattgt gttggcataa aagtccttga tgctgcgata aaaataccga aaatcttgcc
     6721 gattgaagcg cgtttacatc gtgaaattac gggaatgctg aaaagtatca cgttgagccg
     6781 cagcgcaacc gggaaatact acgcgtcgtt actctgtgac gacgggatgg aagcgccggc
     6841 aaagcccgca ctgatatcaa atgtgacagg gctggatatg gggctggcgc attttgtcat
     6901 caaatcggac ggcaataaaa ttgccagtcc ccgccaccta atcaatgcaa gtcgcaacct
     6961 gcgccggaag cagaaagcac tgtcccgtaa gaaaaaaggg agcgcaaaca ggcgcaaagc
     7021 cagaatacaa cttgcagccg ttcacgaacg ggtagccggt gcccgtgccg atttccagca
     7081 caagttatct cgcgcaattg ttgacgaaaa ccaagcggta attgtcgaga cattgaaaat
     7141 ggcaaatatg atgaaaaatc accatctggc tcgtgcgata ggcgatgctg gatggtctgg
     7201 attcatcaca aaactggaat acaaagcaac agagaaaggt gtgcatctgg tgaaactgga
     7261 tcaatggttc gccagttcga agacctgcca ctgctgcggt cacaaaatgt cggagatgcc
     7321 attacataag cgcatctggc agtgtccaga gtgtggagtt gaacatgacc gcgatatcaa
     7381 cgcggcgctc aacatcaaac gcaagggcat actggaatta caagcggcgg gactcgtcgt
     7441 ttctgcccat ggaggccagc gtaaatccgt cacacagacg gttgcggctt aagaagtggg
     7501 aagcctcgcc cgacagggcg gggagcagtc acatgatgac aggtaatggc gcggatatcg
     7561 aatgttatgc agacagagaa aatcagcctg ctcaaatggc tgtgcggttc tggatagccc
     7621 gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt gcgtttttgt
     7681 tgttcgagat cgcgttcaat gccttcacga acgtcctggg ggattttcag cgcgtcaccc
     7741 agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc gcagctcagg
     7801 aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg ttgtggatcc
     7861 agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac gccggcttca
     7921 cgcaattgct gatcgattgc cgcacgttct ttggcatcaa tatgaccatc acttttagcg
     7981 gcaaagacca gcgcaaggat caaacgttca gtacgctcat ccagcggcgt actttgcgcg
     8041 ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt attccacagc
     8101 accgtacccg ctaccgctcc gccgccaacc agcaacgcgt tggtgccata tttcgtaaga
     8161 agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa tgcgcccggc
     8221 actaaaagtt tgcccaatcc ctgatccgca gacgaggagg cagaagaact gctttgccca
     8281 agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata accattttta
     8341 gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa aagatgcgca
     8401 aataagcggg gcggttggcc gttattcaaa gaaattcccg ccaggtaagg tgttcactgt
     8461 atatcagatg ataagggtat tttaggtgaa agtagtttga cgttttgagt agcactgttt
     8521 gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt
     8581 gtggggcgga taaggcactc gcgccgcatc cgccaacggt caatcgaatt atcagaagcc
     8641 attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc tgataatgaa
     8701 tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct ttaccctgaa
     8761 tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt tgatcatctg
     8821 cccagcgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca attccggcag
     8881 catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta ttggcagcga
     8941 aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt tttttcatgt
     9001 ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag gagcgcaagt
     9061 tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg acctgtgggg
     9121 acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct gccagccata
     9181 gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc agccaggtgt
     9241 caagcagatc cgccgtatca agcattttgt cataagcatc ggcttccttt ttgctggtaa
     9301 acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc gccatttgca
     9361 gcctctcata ataactgtga ttttatacag tatatttctt ttcggttgga aaatcaatat
     9421 cagcaataaa gacacgcgca aacgttttcg tttatactgc gcgcggaatt aatcagggga
     9481 tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat
     9541 taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg
     9601 tgattgccgt cgatcgctat gccgacgcgc cagctatgca tgtcgcgcat cgctcccatg
     9661 tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt
     9721 atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaag
     9781 gactgaatgt tgtcccctgc gcgcgcgcaa cgaaattaac gatgaatcgc gagggtatcc
     9841 gtcgcctggc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt gccgataacg
     9901 aaaacctttt ccgcaaggcg gttgctgaaa tcggctatcc ctgcattgtt aaaccggtga
     9961 tgagttcctc cggcaagggg cagacgttta ttcgttctgc agagcaactt gctcatgcat
    10021 gggagtacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg
    10081 ttaagtttga tttcgaaatt accctgctaa ccgtcagcgc ggtggatggc gtccatttct
    10141 gtgcaccggt aggtcatcgc caggaagatg gcgactatcg tgaatcctgg caaccacagc
    10201 aaatgagcca gctggctttg gaacgtgcgc aagaaattgc ccgtaaagtg gtgctggcac
    10261 tgggcggtta tgggctgttt ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca
    10321 gtgaggtctc ccctcgccca catgataccg ggatggtgac gttaatttct caggatctct
    10381 cggagtttgc cctgcatgtg cgagcctttc tcggacttcc ggttggcggg atccgtcagt
    10441 atggtcctgc agcttctgcc gttatcctgc cacaactgac cagtgagaat gtcacgtttg
    10501 ataatgtgca gaatgccgta ggcgcaggcc tacagattcg tttatttggt aagccggaaa
    10561 ttgatggcag ccgtcgtctg ggtgttgcgc tggcgaccgc tgagagtgtt gatgaagcga
    10621 ttgagcgcgc aaagcacgcc gcagcacaga taaaagtaca agcttaaccc cggcgaaaaa
    10681 cgctaccaaa atgcccgatc gaggatcggg cattttgact tttacagctt agcgccttct
    10741 acagcttcac gcgccagctt agtaatgcgg tcgtaatcgc ccgcttccag cgcatctgcc
    10801 ggaaccagcc aggaaccacc gatgcacagc acgcttttca gcgccaggta gtcacggtag
    10861 ttagccggag aaataccacc cgtcgggcag aaacggacct gggagaacgg acccgcgatc
    10921 gcctgcaggg ctttcacacc gccgttagct tcagccggga agaatttgaa ctctttcaaa
    10981 ccgtagtcca tacccagcat cagttcggaa acagtgctga tccccggaat cagaggaata
    11041 gtcccttcgg tagcagcctt cagcagcggc tcggtcagac ccgggctaat tgcgaactgt
    11101 gcacccgctt cggtgacttc tgccagctgc tgtggattca gcaccgtacc ggcacccaca
    11161 atcgcttcag gcacttcttt ggcgatagca cggatagcgt caactgcaca ctcggtacgc
    11221 agagtcactt ccagaacgcg caccccacca gcaaccaacg cttttgccat cggcaccgcg
    11281 tgttccagtt tttttaccac gataaccggt acaaccgggc cggtggtcag gattgattct
    11341 gcacttgttt tccagttttt catcagagtt ttctctcgcc tgattacaaa tttgtcgtct
    11401 taaaaagtga tacaggttgc gccctgttcg gcaccggaca gtttttcacg caaggcgctg
    11461 aataattcac gtcctgttcc cacgcgtgac gcgctcaggt caggaatgtg tggttcgcga
    11521 gcagccagtt ccgcttcgtc taccagcagc gtcagttcgc ctgtctgtcc attcacacga
    11581 atgatgtccc cgtcgcgcac ttttgccagc agcccgccat cgtaggcttc tggtgttacg
    11641 tggatagctg acggcacttt acctgaagcg ccggagagtc gtccatcagt aactaacgca
    11701 attttgaaac accggtccaa taatacacca agtggcggca tgagtttatg taattctggc
    11761 attccgttcg cttttggccc ctgatgacgg acaacaacga cacaatcgcg gtccagcaaa
    11821 cccgcttcaa aagccggcat aacgtcatgc tggctttcaa aaacaaccgc aggcgcttca
    11881 atcacctggt tctcaaccgg cacggcagag gttttcataa ccgcacggcc caggttaccg
    11941 cttaacactt ttgtcccacc atgatgagag aaaggttgtt cgaaggaagc gatcacattg
    12001 ctgtcgagtg atttttccgc cccttcccgc cagtccagtt caccattatt cagccatggt
    12061 tcaagggtat aacgagacag accaaaacca gccaccgtat tgacatcttc atgcagcagg
    12121 cctgctttga gcagttcacg caccagaacc ggtacgccac ctgccgcctg gaagtggtta
    12181 atatcggccg gaccgttcgg gtagagacgt gccatcagcg gtacaacatc agaaaggtca
    12241 gagaagtcat cccagttaat ctgaataccg gccgcgcgag ccatcgccac caggtgcatg
    12301 gtgtggttag tggaaccacc ggtcgccagc agtgcaacga taccgttcac caccactttc
    12361 tcatcgatca tcttaccgat cggcatccat tcattaccat taccggtcat gcgtgtaacc
    12421 tggcgcgcag cggctgcggt caaagcatcg cgcagcggag aatccggatg aacaaaagaa
    12481 gagcctggca actgcatccc cataaactcc accaccatct ggttggtgtt ggcagtaccg
    12541 tagaaagtac atgttcccgg cgcatgataa gacgcggctt ctgactccag taatgccatg
    12601 cggtccactt taccttcggc ataaagctgg cgaatacgca ctttttcttt atttggcaaa
    12661 ccgcttgcca tcggtccaga cggcacaaac accgcaggca aatgcccaaa cgacagggct
    12721 gccatagtca gacccggaac aatcttgtcg cacactccga ggaacagcgc accatcaaac
    12781 atgttatggg acaaccccac cgccgcagac atcgcaatca cttcgcggct taacagcgac
    12841 aattccattc catcctgccc ctgggtgaca ccatcacaca tcgccggaac accgcccgca
    12901 acctgaccaa ccgcatttgc ttcatgcagg gctttacgaa tgatttctgg atagtgttca
    12961 taaggctggt gcgcggagag catgtcgtta taggaggtga tgatggcgat attgttacgc
    13021 aacatgcttt tcaaagaggc tttgtcttct ggctggcagg cagcgaaacc gtgtgccagg
    13081 ttaccacatg ccaactgcga acgatgaacg gtcgaagttt tcgcttgttc tatccgggcg
    13141 aggtaagcag agcgagtctc gcgcgaacgt tcaatgattc gatttgttac gcgtaacaat
    13201 tgtggattca taaaggctcc tgaaattgag ttgtcagagc aggatgattc acaacgcgct
    13261 tcattcagag gatttatgac tgaaacgcct gtaaccggag ctcatagggc aaaaacgttt
    13321 cagtcagtgt aataaaaaaa gcctcgtggg tgaatccgca cgaggcctga aagtgtaaaa
    13381 attgttctac aatctgtgca agatcatgtt accggtaaaa taaccataaa ggataagcgc
    13441 agatattact caaactcatt ccaggaacga ccatcacggg taatcattgc caccgaggca
    13501 acgggtcccc aggttccggc ctgatacggt ttcggcgcat cattgtccat cgcccacgcc
    13561 tcagtaatgg agtctaccca tttccaggct tcttccactt catcacgacg aacaaacagt
    13621 gcctgaatac cacgcatggt ttccagcagc agacgttcat aggcatccgc cagatgcgtc
    13681 tgattaaagg tttctgaata gctcaggtcc agcttggtga tttgcaggtt atgtttgtgg
    13741 tcaaggccag gaactttatt cagtacctgg atatccacgc cttcatcagg ttgcagacgg
    13801 atagtcagtt tattttgcgg tagatcctgc cacgactctt taaacagatt cagttcaggt
    13861 gttttgaaat agaccacgac ttcagagcat ttggtcggca gacgtttacc agtacgcagg
    13921 tagaatggca caccggccca gcgccagtta tcaatgtcga cgcggatcgc cacgaaggtt
    13981 tctgtattgc tgctcttgtt cgcgccctct tcttccagat atcccggcac ttttttgccc
    14041 tgggcgaagc ccgcagtata ttgcccgcgt acggtttttt cgcgtacgtt ggagcggtca
    14101 atacggcgca gagacttcag tactttcact ttttcatcgc ggatgctgtc tgcactcagg
    14161 tcagacggcg gagacatcgc aatcatgcaa agaatttgca gcaggtggtt ctggatcatg
    14221 tcgcgcatct gaccggcttt atcaaaatag ccccagcgcc cttcgatccc cacttcttct
    14281 gccacggtaa tctcaacatg atcgatggtg cgattgtccc agttgttcac aaacagagag
    14341 ttagcaaaac gcagcgccaa caggttcagc accgtttctt taccaagata gtggtcgata
    14401 cggtaaacct ggcactcctc gaagtattcg ccaacctgat cgttgatttc ctgcgaggtc
    14461 gccagcgacg tccccagcgg tttctccatg actacgcgtg ccggtttagc attcagtttt
    14521 gcctcgccaa gccctttgca aattgcgcca aaagtgctgg gcggcatggc aaagtagtta
    14581 atggtgatac gatttttttg atccagcatc gcgccgagac ggctgaatgc agcagtgtca
    14641 ttgacatcga gattacaaaa atccagacgt gcactcaggg tgtcccataa accttcatca
    14701 atggtttctt tcatgaaagt ttcgagcgcc tcgcggacaa ctttggtata agccgcttta
    14761 tcccagtcag cacgccctac gccgataatc cgggtgtccg ggttgagctg accggctttt
    14821 tccagttgat acagggaagg cagcaattta cgacgcgcaa ggtcgccttt cgcgccgaaa
    14881 atgaccaggt cacaggcctg ggctgtttgc gttaccgcca tgtcattctc cttaagttaa
    14941 ctatcccggt acttaagcca gggtatactt gtaattttct tacggtgcac tgtactgctt
    15001 ttacgagctt gcgaaaactg taaacgctta tccacccgtg cgattacggt aaaagcgcgc
    15061 aaagtgcggc aaaactgata aaaaaatcat cgttttccat tgggtaaaaa tctgacactg
    15121 atcatgttat gaaaaaaaat aacaactttt ttatctgctt ttgtcattaa cacagcacac
    15181 aggcgtaata tctgacaaaa ctgcatttcg atttctttca gtgcggaaat cgtcattacc
    15241 cgtgagtctc tttacatcat gaatatgctg gaaaaaatcc agtctcagct ggaacatttg
    15301 agcaaatcag agcgcaaagt tgccgaggtc attctggctt cgcccgataa cgcgatccat
    15361 tcgagtattg ctgctctggc gctggaagcc aatgttagcg aaccgacggt gaatcgtttc
    15421 tgtcgcagca tggacacgcg cggttttcct gattttaaac ttcatctggc acaaagtctg
    15481 gcgaatggca ctccctatgt taatcgcaac gtcaatgaag atgacagcgt tgaatcatac
    15541 acggggaaaa tatttgagtc cgcaatggca acgctcgatc atgtccgtca ttcattggat
    15601 aaatctgcca tcaaccgcgc cgtcgactta ctcactcagg caaaaaaaat cgcctttttc
    15661 ggattaggct cttctgccgc tgttgcccac gatgcgatga ataagttctt tcgttttaat
    15721 gttccggtgg tgtactccga tgatattgtg ctgcaacgca tgagttgtat gaattgtagc
    15781 gacggagacg tggtggtgct gatttctcac acgggaagaa caaaaaatct ggtcgagctg
    15841 gcgcagctgg cacgcgaaaa cgacgccatg gtgattgccc tcacctctgc aggtaccccg
    15901 ctcgcccggg aagccacgct agcaattacc ctcgacgtac cggaagatac tgacatttat
    15961 atgcccatgg tttctcgact tgcacagctg accgtgatag atgtgctggc gacaggattt
    16021 actttgcgac gcggtgcaaa attcagagat aacttgaagc gggtcaagga agcgctgaag
    16081 gaatcgcgtt ttgataagca gttacttaat ttaagtgacg atcgctaaaa acgactgtca
    16141 ctgtcctaat cttatacgac atccgaatga gattaattta tagccatcgc ggcgttattt
    16201 cattcggatt tcatgttcaa gcaacacctg gttgtttcag tcaacggagt attacatgtc
    16261 cagaaggctt cgcagaacaa aaatcgttac cacgttaggc ccagcaacag atcgcgataa
    16321 taatcttgaa aaagttatcg cagcgggtgc caacgttgta cgtatgaact tttctcacgg
    16381 ctcgcctgaa gatcacaaaa tgcgcgcgga taaagttcgt gaaattgccg caaaactggg
    16441 gcgtcatgtg gctattctgg gtgacctcca ggggcccaaa attcgtgtat ccacctttaa
    16501 agaaggcaaa gttttcctca atattgggga taaattcctg ctcgacgcca acctgggtaa
    16561 aggtgaaggc gacaaagaaa aagtcggtat cgactacaaa ggcctgcctg ctgacgtcgt
    16621 gcctggtgac atcctgctgc tggacgatgg tcgcgtccag ttaaaagtac tggaagttca
    16681 gggcatgaaa gtgttcaccg aagtcaccgt cggtggtccc ctctccaaca ataaaggtat
    16741 caacaaactt ggcggcggtt tgtcggctga agcgctgacc gaaaaagaca aagcagacat
    16801 taagactgcg gcgttaattg gcgtagatta cctggctgtc tccttcccac gctgcggcga
    16861 agatctgaac tatgcccgtc gcctggcacg cgatgcaggc tgtgatgcga aaattgttgc
    16921 caaggttgaa cgtgcggaag ccgtttgcag ccaggatgca atggatgaca tcatcctcgc
    16981 ctctgacgtg gtaatggttg cacgtggcga cctcggtgtg gaaattggcg acccggaact
    17041 ggtcggcatt cagaaagcgt tgatccgtcg tgcgcgtcag cttaaccgcg cagtaatcac
    17101 ggcgacccag atgatggagt caatgattac taacccgatg ccgacgcgtg cagaagtcat
    17161 ggacgtagca aacgccgttc tggatggtac tgacgctgtg atgctttctg cagaaactgc
    17221 tgctggtcag tatccttcag aaactgttgc agccatggcg cgcgtttgcc tgggtgcgga
    17281 aaaaatcccg agcatcaacg tttctaaaca ccgtctggac gtgcagttcg acaatgtgga
    17341 agaagctatt gccatgtcag caatgtacgc ggctaaccac ctgaaaggcg ttacggcgat
    17401 catcaccatg accgaatcgg gtcgtacggc gctgatgacc tcccgtatca gctctggtct
    17461 gccaattttc gccatgtcac gccatgaacg tacgctgaac ctgactgctc tctatcgcgg
    17521 cgttacgccg gtgcactttg atagcgctaa tgacggcgta gcagctgcca gcgaagcggt
    17581 taatctgctg cgcgataaag gttacttgat gtctggtgac ctggtgattg tcacccaggg
    17641 cgacgtgatg agtaccgtgg gttctactaa taccacgcgt attttaacgg tagagtaagt
    17701 acgttgccgg atgcggcgaa aacgcctcat ccggcctaca gttcaattat agttcaacag
    17761 atttcgaatc ttctgaagca aacttgaact tatcacaagg cggaggcctc tcctcgcgag
    17821 aggctttttt tatttgatgg gataaagatc tttgcgctta tacggttgga tttcgcccgg
    17881 cttgcgagtt ttcagcaatt ttaatatcca ggtgtattgt tctggtcgcg gaccaacaaa
    17941 aatctcgact tcttcattca tccgccgcgc aatcgtatgg tcatctgcct ctaacagatc
    18001 atccatcggt gggcgcacct gaatcgtcag gcgatgcgtc ttgccatcat aaatcggaaa
    18061 cagcggtaca acgcgcgcac ggcacacttt catcaaacga ccaatcgcgg gcaacgtcgc
    18121 tttataggtg gcaaagaaat caacaaattc gctgtgctct gggccatgat cctgatcggg
    18181 taaataatat ccccagtacc cctgacgtac cgactggatg aatggtttaa taccatcatt
    18241 tctcgcatgc agacgaccgc caaaacgacg acgcaccgtg ttccagacat aatcaaaaac
    18301 cgggttgccc tgattatgga acatcgctgc cattttctgc ccttgcgagg ccatcagcat
    18361 ggcaggaata tcgacggccc aaccgtgcgg caccagaaag atcactttct cgttattacg
    18421 tcgcatctct tcgataatct ccagcccttg ccagtcaacg cgcggctgaa ttttctccgg
    18481 cccgcgtatt gccaactcag ccatcattgc catcgcttgc ggcgcggtgg caaacatctc
    18541 atcaacaatc gcttcgcgtt cagcttcact acgttctgga aagcagagcg acaaattgat
    18601 taacgcacga cggcgtgagc tttttcccag gcgtccggca aaacgtccca gccgtgccag
    18661 aatggggtcg cgaaactttg gcggcgttaa agcaataccc gccatcgctg ctacgcccag
    18721 ccatgctccc cagtagcgcg ggtggcgaaa ggatttatca aactcaggaa tgtattcgct
    18781 attatttttt ttcgtttcca tgcttttcca gtttcggata aggcaaaaat caatctggtg
    18841 atagtgtagc ggcgcaactt gccccgcacc
//
LOCUS       DARNVM010000092        18583 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.092, whole genome shotgun sequence.
ACCESSION   DARNVM010000092 DARNVM010000000
VERSION     DARNVM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.092"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..896
                     /locus_tag="SI621_002873"
     CDS             <1..896
                     /locus_tag="SI621_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEI4065563.1"
                     /translation="NALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYI
                     KEVFVSSGARVTAQDVPIAGGPYKNIVADYGPADGPLIIIGAHYDSVSSYENDQLTYT
                     PGADDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLER
                     PVRLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAAL
                     LSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDTADR
                     LNYQKMAQVVDGVITLLYNSK"
     gene            999..1502
                     /locus_tag="SI621_002874"
     CDS             999..1502
                     /locus_tag="SI621_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HEI4065564.1"
                     /translation="MGMIGYFAEIDSDKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPANNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1570..3027)
                     /gene="nuoN"
                     /locus_tag="SI621_002875"
     CDS             complement(1570..3027)
                     /gene="nuoN"
                     /locus_tag="SI621_002875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEI4065565.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3034..4563)
                     /gene="nuoM"
                     /locus_tag="SI621_002876"
     CDS             complement(3034..4563)
                     /gene="nuoM"
                     /locus_tag="SI621_002876"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEI4065566.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4729..6570)
                     /gene="nuoL"
                     /locus_tag="SI621_002877"
     CDS             complement(4729..6570)
                     /gene="nuoL"
                     /locus_tag="SI621_002877"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEI4065567.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARSHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     ITHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6567..6869)
                     /gene="nuoK"
                     /locus_tag="SI621_002878"
     CDS             complement(6567..6869)
                     /gene="nuoK"
                     /locus_tag="SI621_002878"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEI4065568.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6866..7420)
                     /gene="nuoJ"
                     /locus_tag="SI621_002879"
     CDS             complement(6866..7420)
                     /gene="nuoJ"
                     /locus_tag="SI621_002879"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEI4065569.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7432..7974)
                     /gene="nuoI"
                     /locus_tag="SI621_002880"
     CDS             complement(7432..7974)
                     /gene="nuoI"
                     /locus_tag="SI621_002880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEI4065570.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7989..8966)
                     /gene="nuoH"
                     /locus_tag="SI621_002881"
     CDS             complement(7989..8966)
                     /gene="nuoH"
                     /locus_tag="SI621_002881"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEI4065571.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8963..11689)
                     /gene="nuoG"
                     /locus_tag="SI621_002882"
     CDS             complement(8963..11689)
                     /gene="nuoG"
                     /locus_tag="SI621_002882"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEI4065572.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11742..13079)
                     /gene="nuoF"
                     /locus_tag="SI621_002883"
     CDS             complement(11742..13079)
                     /gene="nuoF"
                     /locus_tag="SI621_002883"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEI4065573.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13076..13576)
                     /gene="nuoE"
                     /locus_tag="SI621_002884"
     CDS             complement(13076..13576)
                     /gene="nuoE"
                     /locus_tag="SI621_002884"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEI4065574.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13579..15381)
                     /gene="nuoC"
                     /locus_tag="SI621_002885"
     CDS             complement(13579..15381)
                     /gene="nuoC"
                     /locus_tag="SI621_002885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEI4065575.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15475..16137)
                     /gene="nuoB"
                     /locus_tag="SI621_002886"
     CDS             complement(15475..16137)
                     /gene="nuoB"
                     /locus_tag="SI621_002886"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEI4065576.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16153..16596)
                     /gene="nuoA"
                     /locus_tag="SI621_002887"
     CDS             complement(16153..16596)
                     /gene="nuoA"
                     /locus_tag="SI621_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HEI4065577.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17226..18164)
                     /gene="lrhA"
                     /locus_tag="SI621_002888"
     CDS             complement(17226..18164)
                     /gene="lrhA"
                     /locus_tag="SI621_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HEI4065578.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4617 a         5158 c         4823 g         3985 t
ORIGIN      
        1 tcaatgcatt accgccaacg ccccgacacg caagtcctga gcaattagaa aaaacagttc
       61 gttatcttac acaaactgtg catccacgta gtgccgacaa tattgataat ctgaataggt
      121 ccgcagagta tataaaagag gtctttgtca gtagcggtgc cagagttacc gcgcaggacg
      181 tccccattgc gggtggcccc tacaaaaaca ttgttgctga ttatggtcct gccgatggac
      241 cgctgattat tattggtgcg cactatgact ctgtcagtag ttatgaaaat gatcaattga
      301 cctatacgcc gggcgcggat gataacgcca gcggcgtggc aggattactc gaactggcac
      361 gtttgttaca gcagcaagca ccgaaaactg gcgtgcagct ggtcgcctat gcgtcggaag
      421 aaccgccctt ctttcgtagc gatgaaatgg ggagcgcggt acatgcagct tcgcttgagc
      481 gtccagtgag attaatgata gcgctggaga tgattggcta ttacgactct gcgcctggta
      541 gtcaggatta cccttacccg gcaatgtcct ggctttatcc cgatcgggga gactttattg
      601 ccgtggtcgg cagaatgcag gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt
      661 catcccggga tttatctgtt tattctatga atgccccagg gtttattccc ggtattgatt
      721 tctctgacca cctgaattat tggcaacacg atattcccgc agtaatgatt actgacaccg
      781 ctttttatcg taataaacaa taccacttgc ccggtgatac cgcagacaga ttgaattatc
      841 agaaaatggc tcaggtagtg gatggggtta taactttgtt atacaacagt aaataataaa
      901 aatttctcga ggcgggtcca gaaaaagcga catgtacatt ttattttccc tcgtctgttt
      961 taagatgcga aacatgaaat tcaacggagg aaaataaaat gggaatgatt ggctattttg
     1021 cagaaattga ctcagataag attaaccaac tgcttgagtc tactgaaaaa cccttaatgg
     1081 acaatattca tgacacgctt tcaggattac gccgactgga tattgataaa cgctgggatt
     1141 ttttacactt tgggctgaca ggcacctctg cttttgatcc cgcgaataat gatccgctga
     1201 gtcgtgccgt gcttggtgaa cacagcctcg aagatggcat agatggcttc ctgggattga
     1261 cctggaatca ggagttagcc gcgacaatcg accgactgga agctctggat cgaagtgagc
     1321 tgcgtaaaca gttttccatt aaacgactca atgaaatgga gatttaccct ggtgtgactt
     1381 tttcagaaga gttagagggg caactctttg cttcgatcat gcttgatatg gaaaagctga
     1441 ttagtgcgta tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt
     1501 gatggggtca gggcaccaaa aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa
     1561 actgactgct tacatcagcg gcatggccaa ccgcacaatg ctaatcagcg gttgtggcca
     1621 tacacccagc accagtacca acagtgcaga gatcagcacc acgataccgc ccgcgctgta
     1681 ctgccagttt gaaggtgcat cgcgacccgg ttgttccggg gcgtgaagat acaagctcac
     1741 cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag
     1801 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccaa taaagcccag
     1861 cgtcatcgga ataccggcca gagacagcat catcaccgtc atcaccgccg cgaggatcgg
     1921 gcgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     1981 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag
     2041 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     2101 cagatagccg aggtgagaga tagatgagta accgagcaaa cgtttgatat tggtctggct
     2161 cagcgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gcaccacccg
     2221 aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca caccgaagat
     2281 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac
     2341 gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat
     2401 caggccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc
     2461 cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac cgaacagcag
     2521 gaaagaagac gctgcggcag agagaatggt gtatttgata cttgcttcca gcgaacgttt
     2581 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag
     2641 gaacaaagac gccagatggt tggcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2701 caccaacagg tagaactcgt ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2761 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2821 aacgcgcatc agtggcgtaa cgtccatagc gcccgcctgg ccgacaaacc agagcgaaac
     2881 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     2941 cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg gtagcagtgc
     3001 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     3061 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     3121 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3181 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg
     3241 atgtaacatc gccagcgaat aaacagaggc aaagaccagc ccaaaggtag agataacggt
     3301 aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc
     3361 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg gcagccattt
     3421 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3481 ctgaccgcag agaataaaca gacccgccgc tgacaagccg tgcgcaatca tctggattac
     3541 cgcgccctgg taggccaact ggctgccagt gtagatagca atcagcacga agcccatgtg
     3601 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3661 accgtagaag atgccgataa cacccagcca catggcgatt ggcgcgaact ctgccgacgc
     3721 gttcgggaac agcggtaggg agaaacgcag cagaccgtaa gcggcggttt tcagcaagat
     3781 ccccgcgagg tccacggaac cggcggttgg agcctgtgag tgcgcatccg gcagccagcc
     3841 atgcagcgga accaccggca ttttcaccgc aaatgcgatg aagaagccca gcatcaacag
     3901 atattccaca ccactggaca ttggcgtatt caacagctct tcatagttga aggtccagac
     3961 gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcgatcaaca tcaccagacc
     4021 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     4081 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     4141 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4201 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac
     4261 cgccagcaca ccgagcagac cagtcagcac gaccatcagc agcgatagcc cgtcaatggc
     4321 gagatggata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4381 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4441 cgccagcgtc aatcccatgg tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg
     4501 ttcggtctgc cagcacagga agccgccgat aaagggaatt aatattagcc agggtagtaa
     4561 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4621 cgacagaaaa ttctcttcgg attgtggttg tagtgtcgga tgcgccgcga aggcggctta
     4681 tccgacctac gttcttatat gcccaggggc aagcccacaa tccttaactc aacgcagtac
     4741 catcaacagt gccagcacca cgaccgcacc gatgctcatg gatgccacat accagcgcag
     4801 ataaccgttc tcgcttaaca gcagaccttt acctgcaaag cgggaaagga cagccgggat
     4861 gttcatcatt gagttcagcg gatcgcgttt cagcaaccag gcaataccca ggaacggctt
     4921 gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag
     4981 cagacggccc ggcgcactgt tggcgatgga agtcaccaga gtacgtttac ccagccacag
     5041 ccaggctgcc agcagaatgc cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa
     5101 catgctgccg tgcgccagct ccgtcgtttg cgggagcacg ccctgcagcg gcggtacaat
     5161 cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc agcggcaggc tgtgagttat
     5221 ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg aagacgatga aaatcatacg
     5281 gaaggtgtag agcgaggtca taaacgcacc gaccagacca gccaccatca gattgatatg
     5341 accattcgcc atcgcacccg cgaggatctc atccttactg aagaagcccg cagtgaccag
     5401 cggcagtgcc gacagtgctg cgccgcccac caggaagcag agataaacca gcggaataga
     5461 tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga
     5521 accggatgcc aggaacagca gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc
     5581 cgcatcccat gcctgcacgc caagcgcgag gaacatgtag ccaatctggc tcatggtaga
     5641 gtaagcgaga acacgtttga tatcggtctg taccagcgcg gcaaaaccgg ccagcagcag
     5701 cgtaaccgcc ccaacaatgc ccaccagatg cagaacttcc ggcgtcatca ggaacaggcc
     5761 gtggctacgg gcgatcaggt agacacccgc ggttaccatg gttgcggcgt ggatcagcgc
     5821 ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc
     5881 agatttaccg accgcaccgc ccagcagcat cagcgtcgcc cacatcagca tgttattgcc
     5941 gtcagcaaag tgcgctggtg ccagttccac catttcgcgg aagttcaggg tgcccagttc
     6001 gttgtaaaga atgaacagcg cgaaagcgag gaagacgtca cccacacggg tcacgacgaa
     6061 tgccttcatt gccgctgcgc cattcttcgg atcggtgtaa tagaaaccga tcagcagata
     6121 ggagcacagg cccacgcctt cccagccgag gtacatcagc agcaggttgt cggcaagcac
     6181 cagaactacc atgctggcga tgaacaggtt ggtgtaagcg aagaagcgag agtagccctc
     6241 ttcaccgcgc atataccagg aggcgtacat gtgaataagg aaacccacac cagtgaccac
     6301 cgagagcatg gtcagcgaca ggccgtccag caccaggtta aaaccgatgt taaagtcgcc
     6361 taccgacatc cacgtccaca gcggctggct gtatgcctgc tcaccgttag cgaagaagtc
     6421 aacgccgata aaggcagtta ccagcgccgc caggcccaca gagccaacgc cgacgatcgc
     6481 cgagacgttt tcagaccagc gcccacggga gaacgccagc aggacgaagc caatcaatgg
     6541 caaaataatg gttaaggcaa gcatgttcat ccgcgcatct cacttactga atcaatgttc
     6601 aggttctggc gacgacggtg aagttgcagc agcagcgcta ggccgatact cgcttctgcc
     6661 gccgcgaggc tgatggcgag aatgtacatc acctgaccgt cggtctggcc ccagtagctt
     6721 ccggcgacca caaaggccag cgcggaggcg ttaatcatga tttccagacc aatcagcata
     6781 aacagcagat tgcgacggat aaccagacca gttaagccaa gaatgaataa gattgccgcg
     6841 aggatcagtc catgttgtaa ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt
     6901 cgtctttacg attgctcagc acttcacccg cacgctcttc acgaccgacg tggaaggcca
     6961 caaccagacc cgcgagcagc agcatagaag ccagttccac cgccagtacg taaggcccga
     7021 acagcgtaat acccactgct ttagcactga ttggcgtacc gtcgataccc tgatcgttaa
     7081 caccgaggat ggcgtaaaca atcaccacca gcatgatggc cgacaaaatt gccggaccaa
     7141 tccacacctg cggtttcagc cactggcgtt cctgttcaat ttctgaaccg cccaggttga
     7201 gcatcatcac cacgaacacg aacagcacca taatggcacc cgcgtagacg ataatttcca
     7261 gcgcaccggc gaagtaagcg cccagtgaga agaacacccc agagatcgcc agcagcgaaa
     7321 taatcaggta cagcagtgcg tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa
     7381 gtatggctat caggccacag atataaaaag cgaactccat tgcccctctc cttacggtaa
     7441 caggctcttg acgtcgatag gcttggcttc gttctctgct tcgcccttat ctttgccgtc
     7501 gattgccata cctgccatcc ggtagaagtt atattccggg tatttgcccg gaccggagat
     7561 cagcagatcc tctttctcgt aaaccagatc ctggcgcttg tattccccca tttcgaaatc
     7621 cggggttaac tgaatcgccg tggtcggaca ggcttcttcg cacagaccac agaaaatgca
     7681 gcgtgagaag ttgatgcgga aaaattccgg ataccagcga ccgtctttgg tttctgcttt
     7741 ttgcagcgag atacagccga ccgggcaggc taccgcgcag aggttacagg ctacgcaacg
     7801 ctcttcgccg tccgggtcgc gggtcagaac gatacgacca cgataacggg gcggcagata
     7861 gaccggttct tccgggtaca ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat
     7921 ccagatacta cgaacctggg tgccgaatcc tactaacaat tcttttaagg tcatggtctt
     7981 attgcccctt attgcgcctg ccagagaatg acagccgccg ttaccagcaa gttgatcagc
     8041 gtcagcggca ggcagatttt ccagccgaag gacattacct ggtcataacg cggacgcggt
     8101 aacgacgcac gaatcaaaat gaacatcatc ataaagaacg cggttttcag cgcgaaccag
     8161 atgaatggcg gtaacaacgg gccttgccag ccaccgaaga acagcgtcac catcagtgca
     8221 gagatggtca caatcccgat gtattcgccc acgaagaaca gaccgaactt catgccggaa
     8281 tattcaatgt ggtaaccatc cgccagttcc tgctcggctt ccggctggtc aaacgggtga
     8341 cggtgacaca ccgccacgcc cgcgatggca aaggtaataa aaccaaagaa ttgcgggatg
     8401 acgttccaca catgcgcctg gctgttgacg atgtcggtca tgttgaatga accggcctgc
     8461 gccaccacgc ccatcaggga aagcccgagg aacacttcgt agctcagagt ctgcgcagaa
     8521 gcacgcatcg cacccagcag cgagtatttg ttgttacttg accagcccgc aaacagcacc
     8581 gcgtagaccg ccagaccggc catcatcagg aagaacaaaa tcccgatgtt caggtcggca
     8641 accacccaac ctgggctgac tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca
     8701 atcatcggtg ccagggtaaa gatgacgcga tccgagaatt tcgggatcca gtcttcttta
     8761 aagaacatct tgatcatgtc cgcgaccagc tggagcgaac cgccccagcc aacacggtta
     8821 ggtccgtaac ggttctggaa cagacccagc aggcgacgtt cgccaaagct catgaatgcc
     8881 ccacaggtga caaccaccag caggatcacc accgctttga ggatggtcag caggatctca
     8941 atcagttccg gtgatatcca actcattgtt gtgcctcctt gagatcctca agatgcgcgc
     9001 ccgccagcac cggagcaatg ccggacatac ccatcggcaa gcccacctgc cctgccgtca
     9061 gcccttcggc gatttcaacc ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc
     9121 gtgtacccgc gttcacaccc aacttcgcgg catccgctgg gttgagtttg atgtacggct
     9181 gcggcatacg gctctggaag accggagcac gctgtgacaa ttcatcgctg ccaaacaggt
     9241 ggtaatacgg cgcgatacgc catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg
     9301 tgaagtaatc cagaccgttt tcgctggttt caaacagacg cacgcccgga tcgccaaagc
     9361 gcagtttgcc gccaacttcg tcctggaatt tgttccacgc ctgcggggag ttccagcctg
     9421 gcgcccaggc aaacggcact tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg
     9481 agaaggtgaa catggtgtca atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg
     9541 cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa
     9601 tacggaatgt cgcatccggc gcagcatctt tgatacccgc cagttccggg attttcgcca
     9661 caacagcgtc aatcacatgg tcgagctgcg tccagtccac ttcacggctc agcaaggtgc
     9721 tatgcagcga gtgtaaccag cgccagcttt ccagcatgac agttttgctg tcgtaataag
     9781 ccggatcgta aacctggaag aaacgttggg cgcgaccttc attattaatt accgtaccgt
     9841 cgctttcagc aaagctggca gcagaaagta ccagatgggc gttttccata atcgctgtgc
     9901 gttgatggtc aaccaccatc accagcggcg ctttagccag cgcagcattc acgcgggtag
     9961 cggaagcgtg acgatgcaga tcgttttcca gcaccaccac cgcgtcggcg cgtccggttt
    10021 ccagttcggt caacgcttct tcaagcgaac cgccccccat aatgcccagc cccatgctgt
    10081 ttacagagcg agcaatcatg gtgataccaa cgtcagcgcc gcgacctttc agggctttcg
    10141 cgacgttagc cgccgcctga atcacctcta agctaccggc gttcgtcccg gagataatca
    10201 acggtttctt cgcaccagcc agtgcctgca cgatgacgtc gattttgctt tgcagctcag
    10261 gttcaatacc gtcaaccgct ggtgcggagt tgtccagcgc atgggcgatg gcaaaaccta
    10321 aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc cgcgatatca tccagacggg
    10381 tgtcatcaac gttggtaaca aacagcggat gcttcgcacg ttgaccgatg ttgaggattg
    10441 ccgcaatctg ccagtcagcc actttctgtg ctgccgccat ttcgcgcgct ttacctttca
    10501 ccgcctgacg aactgccagc gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca
    10561 ccagtaccgc atcgtaagat tcgatttcgc gcagtgccgg cgtataaatg ccgccttcac
    10621 gtagcacttt cagcgccagt tgcagacgtt cctgctcacc gtgagcgata ccggtataga
    10681 agttttcttc gcccaccagt tcacgcagcg caaagttgct ttctacgctg gcacgcggag
    10741 aaccgatgcc gatcactttc ttcgactggc gcagaatatc tgctgcgccc tgcattgcct
    10801 gttcggcatt gagggtaatg aaatcatcgc cacgacgctg tactggctga cgcggacgat
    10861 ctttcaggtt gacgtaaccg taaccgaaac gaccacggtc gcagaggaag tagtggttta
    10921 ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc ataacgctcg ccggggctga
    10981 tgttacagcc gatggaacat tgctggcaga tgctcggcgc aaactgcata tcccatttac
    11041 ggttgtaacg ctcggagtgc gttttgtcgg tgaatacgcc ggtcgggcaa atttcgacca
    11101 ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt
    11161 cgtgcgcacc gtaaacgccc aggtccgtgc cgtcagcgta atctttgtag taacgcacgc
    11221 agcggtagca ggcgatgcag cggttcattt cgtgagagat gaatggcccc aaatcctgat
    11281 tacggtgggt acgtttggtg aaacggtagc gacggaagct gtgtccggtc atcacggtca
    11341 tatcctgaag atggcagtta ccgccttctt cacataccgg acagtcgtgc gggtggttgg
    11401 tcattaacca ctcgaccaca ctttcacgga actgtttcgc ttcttcatcg tcaatggaaa
    11461 taaaggtgcc atcggaagcc ggtgtcatac aggacatcac caggcgacca cgcgtgtctt
    11521 ccgcgttttg gtattgcttc accgcacact ggcggcaagc accgacgctt cccagcgccg
    11581 gatgccagca aaagtaagga atatcaaggc ccagagacag acaagcttcc agcaagttgt
    11641 ccgctccgtt gacctcgtat tctttgccgt ctacatgaat tgtagccatt agcatgcttc
    11701 cagttttctc agtcagagac tgagcgttaa tcgaaattcg gttaccagcg ctctttcagc
    11761 aggttcggct gaatcccatt aatcaaatgg gtattgctga acggctgttt gattcccgcc
    11821 tcaaattctt cgcggaaata tttgatggcg ctctgtaacg gctccactgc accaggtgcg
    11881 tgggcacaga aagttttacc cgggcctaag aatcgacaca gttgctcaag tgtttcgata
    11941 tcgcccggct gaccttcgcc acgctccagc gcacgcagaa ttttcacgct ccacggcaga
    12001 ccgtcgcggc acggcgtaca ccagccacag gactcacggg cgaaaaactc ttccaggtta
    12061 cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga
    12121 cggcttcccg ctttaccgat actttcgaat tccatcggca gatcaaggtg cgcttcggtc
    12181 aggaagtcgg tgccagcgcc gcctggctgc caggctttga atttcagacc gtcacgcata
    12241 ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc cgaatggcag ttcccacagg
    12301 cccggatttt tcacccgacc ggagaagccc atcagcttgg tgccagcatc tttacttttc
    12361 gagatgttct gataccactc cacgccgtta gcgaggatcg ccggaacgtt acacagggtt
    12421 tcgacgttgt tgacacaggt cggtttgccc catacgccgg aggttgccgg gaaggggggt
    12481 ttcgagcgtg ggttagcacg acgcccttcc agggagttga ttaacgcggt ttcttccccg
    12541 cagatgtagc gccctgcccc ggtatggacg aacagttcga agtcaaaacc tgttcccata
    12601 atatttttgc caagcagacc tgcttcggtg gcttcggcaa tggcacggcg cagattcact
    12661 gccgcttcga tatattcgcc acgcaggaag atgtagccac ggtaagcttt cagcgcaaac
    12721 gcggagataa gcataccttc caccagcagg tgcggcagtt gctccatcag caggcggtct
    12781 ttataggtgc ccggctccat ttcatcggcg ttacatagca ggtaacggat gttcatggat
    12841 tcgtctttcg gcatcaggct ccatttcagg ccagtcgaaa agcccgcgcc gccgcgccct
    12901 ttcagaccag cgtcttttac ctgattaaca atttcgtccg gagacagccc ggtcagcgcc
    12961 ttacgcgcgc cttcgtaacc gtttttgctg cggtattcat ccagccacac tggctgtttg
    13021 tcatcgcgca gacgccaggt cagcggatgc gtttcgggag tacggataat gtttttcatt
    13081 tataccgctc cagcagttca gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat
    13141 cgatcatcat gtttggccct ttatcacagt tccccaggca gcaagttggc agcagcgtaa
    13201 agcggccatc aaatgtcgtt tgccctggtt tgatgttcag ctttttctcg agcgccgcct
    13261 gaataccctg ataaccgttg atatgacaga ccacgctgtc gcaataacgg atcacatggc
    13321 gaccaaccgg ctggcggaag atctggctat aaaacgttgc cacaccttcg acgtcgctcg
    13381 ccggaatacc cagcacatcg gcgatcgcgt ggatcgcgcc atccggcacc cagccacgct
    13441 gcttctgaac gattttcagc gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca
    13501 tttcgtgctc aatcgcttca cgctctgccg cactcagctc aaaagcctcg gtttgtggtt
    13561 gttgattctc gtgcataatt agcggtccac atctgacata acaaaatcga tactgcccag
    13621 ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg
    13681 cgcataactc ggcgtacgga tacgggtgcg ataactcatg gtgctaccgt cgctggtcag
    13741 gtagtaactg ttgatcccct tggtcgcctc aatcatctgg aaagattcat tggctggcat
    13801 caccggaccc cacgacactt gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt
    13861 gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct ttgaacgggc cttccggcat
    13921 gttgttgagg cactgctcaa gaatgcgcag actctgacgc agctcttcca ctttaagcat
    13981 tacgcgggtg tagcagtcag aaacaccacc acccaccggg atttcaaagt cgaagttttc
    14041 atagccagaa tacggacgcg ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc
    14101 cgcgccggtg gtgccccact ccagcgcctc tttcgcgcca taggcggcaa cgccctggga
    14161 acgacctttc agaatggtgt tttgcagcgc cgctttctcg taagacgcca gacgtttcgg
    14221 catccagtcg aggaactcac gcagcaggcg atcccagcca cgcggcaggt cgtgcgctac
    14281 gccgccaata cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc
    14341 gtaaattttc tgacgatcgg taaaggcgaa gaacactggc gtcattgcgc cgacgtcctg
    14401 aataaaggtc gagatataca gcagatggct gttgatgcgg aacagctcag aaagcattac
    14461 gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc agtttctcta cagccagcac
    14521 gtaaggcatt tcgttaacgc agccgccgag gtattcgata cggtcggtat acggaatgta
    14581 gctatgccag gactggcgct cgcccatttt ctccgcacca cggtggtggt agccgatgtc
    14641 tggtacgcag tcgacaatct cttcgccatc gagctgcaaa acgatacgga aagccccgtg
    14701 cgccgacggg tggttcgggc cgaggttgag gaacatgaag tcctcgtttt cggttccgcg
    14761 cttcatcccc cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc
    14821 tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg
    14881 gtgacctttc caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt
    14941 aatgccaaac agatcccagg tctcacgctc ataccagtta gcgttcggga acagtttggt
    15001 gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc ttcaacatga tgtcgcggtt
    15061 acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg
    15121 gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg
    15181 cagtttcttt aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg
    15241 aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc
    15301 gccaatcacc ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa
    15361 gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc
    15421 gcgaaataga tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat
    15481 ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc
    15541 tgattgcata ttggcgcgat aaacgccctg atcgccaacc acccaggaga gcggacgacg
    15601 ttctttgccg atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg
    15661 cgggcagccc gggatataca catcaaccgg gatgaattta tcaacgccct gcacaacgga
    15721 ataaatatcg tacataccgc cagagttggc acaggcaccc attgagataa cccattttgg
    15781 ctccagcatc tggtcataca gacgctgaat aaccggtgcc attttggtaa agcaggttcc
    15841 tgccaccacc atcaggtcag cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg
    15901 cgccacgtca tgcaccgcgg taaacgaagt caccatctca acgtaacagc aggaaagacc
    15961 gaagttatac ggccaaattg agtttttacg accccagtta accatgtcat tgagcttgcc
    16021 cataaacacg tttttgttaa cttcttgctc cagagggtcg gttacgatct cctgcttttg
    16081 cagggggtaa cggtcgttct caccgttggg atctatgcgg gtgagcgtat aatccatctt
    16141 aatgcctcgc ggttagcgtt gacgattagc gatactgttc gtttccgggt tcatacgctc
    16201 gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata cgcaccagat aaaccagacc
    16261 tgccagtaac acaaaaataa aaattgcagc ttccacaaag cctacccagc cgctttcgcg
    16321 gatagaggtt gaccatgcga acagatacag cgcttcaacg tcgaagataa cgaagaacat
    16381 ggccaccaga taaaacttgg cggacaggcg taagcgggcg gagccgaccg agtcgatacc
    16441 ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc
    16501 taccagcatc aggcaacaca ggccaatggc aacgataaga aagatagcga atgcccagtg
    16561 atgagcgatg acttcagtgg atgttgacat actcattgct tactcatcaa aagtagcgcc
    16621 agattcactg ctcttcacgg cagatggacg ccacatcgat tcatggggag gaataaaaaa
    16681 aaccttacaa tcactgtaga aattctttta tacagctaat tgatgtggtc ttttactcct
    16741 ttctataacc ttttgtcaac tttaacaaaa gtttcttcac attagtttac ataatatcaa
    16801 caccattagc atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat
    16861 gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa
    16921 tttgtgtcga ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc
    16981 attccataac aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg
    17041 atttttttca ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga
    17101 acagttttgt atgttctgca cgatacgagg aaggcttttt actaaaaaaa agccgctggg
    17161 gtttaaaaca cccccagcgg ctcgttttta cactattgtc tcagaaatta tctatggtcc
    17221 gtcgattact cgatatccct ttcaatcaac aatgagtcat cgccttccgg agcagacatt
    17281 gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc
    17341 tcattattac tggacgggtc atagcacagc aaatattcgg tatcgggtag cggcggtaag
    17401 ccatctactc cactcagcac acgcaggtct ggactcatca tctcgaccgg ccttgccgtc
    17461 acgccaagac cggcttttac tgccgcacga actgccggaa gcgtcgaggc gacataagcc
    17521 agtcgccatg gaatatctgc tttattaagc gtcgccagca ccatatcgcg aaacgggcta
    17581 ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta ttccgctgcg
    17641 cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgttga aggacgatgg
    17701 gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt
    17761 ttaacgcgga catccagcgc tagtttcgga taaaccgaac tcacgcgatt taacaggaaa
    17821 ggtaagatcg tatcggcaga ttcatctgaa gctccgatag ttaacacgcc ctgaagatta
    17881 ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct ggcgtaacca
    17941 agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct
    18001 ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac
    18061 acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc
    18121 agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta tcacttactg
    18181 gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc catttgtaat
    18241 gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat atgagttgca
    18301 ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca gaatatatct
    18361 tcaggaatcg catatctatt aagttactca ctcttttgta tttatgacat gcgcgtgttt
    18421 gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga
    18481 acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca tttatgcgag
    18541 cattttaccc caaaaacctt ttatttataa gggtcattgc gaa
//
LOCUS       DARNVM010000093        18520 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.093, whole genome shotgun sequence.
ACCESSION   DARNVM010000093 DARNVM010000000
VERSION     DARNVM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.093"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(80..655)
                     /locus_tag="SI621_002889"
     CDS             complement(80..655)
                     /locus_tag="SI621_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HEI4065579.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(716..1165)
                     /locus_tag="SI621_002890"
     CDS             complement(716..1165)
                     /locus_tag="SI621_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HEI4065580.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWFVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(1152..1577)
                     /locus_tag="SI621_002891"
     CDS             complement(1152..1577)
                     /locus_tag="SI621_002891"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEI4065581.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            1791..2660
                     /gene="amiA"
                     /locus_tag="SI621_002892"
     CDS             1791..2660
                     /gene="amiA"
                     /locus_tag="SI621_002892"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HEI4065582.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            2664..3563
                     /gene="hemF"
                     /locus_tag="SI621_002893"
     CDS             2664..3563
                     /gene="hemF"
                     /locus_tag="SI621_002893"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEI4065583.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEAGSPEAALSEFIKVRDWV"
     gene            complement(3497..4621)
                     /gene="eutR"
                     /locus_tag="SI621_002894"
     CDS             complement(3497..4621)
                     /gene="eutR"
                     /locus_tag="SI621_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HEI4065584.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPASG"
     gene            complement(4667..5167)
                     /gene="eutK"
                     /locus_tag="SI621_002895"
     CDS             complement(4667..5167)
                     /gene="eutK"
                     /locus_tag="SI621_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HEI4065585.1"
                     /translation="MINALGLLEVDGMVAALDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(5180..5839)
                     /gene="eutL"
                     /locus_tag="SI621_002896"
     CDS             complement(5180..5839)
                     /gene="eutL"
                     /locus_tag="SI621_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HEI4065586.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(5849..6736)
                     /gene="eutC"
                     /locus_tag="SI621_002897"
     CDS             complement(5849..6736)
                     /gene="eutC"
                     /locus_tag="SI621_002897"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HEI4065587.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(6757..8118)
                     /gene="eutB"
                     /locus_tag="SI621_002898"
     CDS             complement(6757..8118)
                     /gene="eutB"
                     /locus_tag="SI621_002898"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HEI4065588.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(8130..9533)
                     /gene="eutA"
                     /locus_tag="SI621_002899"
     CDS             complement(8130..9533)
                     /gene="eutA"
                     /locus_tag="SI621_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEI4065589.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPNSDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(9530..10756)
                     /gene="eutH"
                     /locus_tag="SI621_002900"
     CDS             complement(9530..10756)
                     /gene="eutH"
                     /locus_tag="SI621_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HEI4065590.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
     gene            complement(10926..12113)
                     /gene="eutG"
                     /locus_tag="SI621_002901"
     CDS             complement(10926..12113)
                     /gene="eutG"
                     /locus_tag="SI621_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HEI4065591.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(12103..12939)
                     /gene="eutJ"
                     /locus_tag="SI621_002902"
     CDS             complement(12103..12939)
                     /gene="eutJ"
                     /locus_tag="SI621_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HEI4065592.1"
                     /translation="MAHDEQWLTPRLHIAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(12950..14353)
                     /locus_tag="SI621_002903"
     CDS             complement(12950..14353)
                     /locus_tag="SI621_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HEI4065593.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIEIAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVNRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(14365..14652)
                     /gene="eutN"
                     /locus_tag="SI621_002904"
     CDS             complement(14365..14652)
                     /gene="eutN"
                     /locus_tag="SI621_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HEI4065594.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(14759..15052)
                     /gene="eutM"
                     /locus_tag="SI621_002905"
     CDS             complement(14759..15052)
                     /gene="eutM"
                     /locus_tag="SI621_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEI4065595.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(15091..16107)
                     /gene="pta"
                     /locus_tag="SI621_002906"
     CDS             complement(15091..16107)
                     /gene="pta"
                     /locus_tag="SI621_002906"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEI4065596.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFTLSHGVAMDGLQVIDPHGNLAMREEFAHLWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPQLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(16104..16907)
                     /gene="eutT"
                     /locus_tag="SI621_002907"
     CDS             complement(16104..16907)
                     /gene="eutT"
                     /locus_tag="SI621_002907"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HEI4065597.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(16904..17605)
                     /gene="eutQ"
                     /locus_tag="SI621_002908"
     CDS             complement(16904..17605)
                     /gene="eutQ"
                     /locus_tag="SI621_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HEI4065598.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(17580..18059)
                     /gene="eutP"
                     /locus_tag="SI621_002909"
     CDS             complement(17580..18059)
                     /gene="eutP"
                     /locus_tag="SI621_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HEI4065599.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGDYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(18072..18407)
                     /gene="eutS"
                     /locus_tag="SI621_002910"
     CDS             complement(18072..18407)
                     /gene="eutS"
                     /locus_tag="SI621_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HEI4065600.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         4123 a         5436 c         4852 g         4109 t
ORIGIN      
        1 aagaatgcca cgcaatccgt ggcattgttg ggcgctattt tacgtgtttt ttcgcggcgg
       61 cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca
      121 gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg aactggtagc
      181 taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt
      241 tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac
      301 cggtgtcagc aggaatatcg ctatccagca catcaatgcg actgatcgtg ccttgatcgc
      361 cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc accacgttgc
      421 cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcggcaatgg
      481 cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg ctcactttgg
      541 tggacgaccc aaaccagttc cacgggttag cggcagacca attaactgac gacatcgtgg
      601 aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac
      661 ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt
      721 ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca
      781 caatatccgc cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca
      841 gccacggttg ccagcaaagt aaaaccacct gcgccaacag caacagaaaa tagagcacat
      901 gccagatacg gggaaatgaa gcccgccgac cactcagcaa aaacgccagc accgccggaa
      961 tgccgggaat caaccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagcg
     1021 cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa
     1081 gcaatagcac aaaccagaag agcaagggta aacgcaggcg accgtgtgca tcataatgga
     1141 caggatgaaa ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg
     1201 gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg
     1261 ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccaaccg attaagcaac
     1321 gcattagcaa tcccacgccc acgaaactct ggatgcacgc caagataata agcagacccg
     1381 cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgttcac ctcagcgacc
     1441 agaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg atcgttccac
     1501 ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga
     1561 aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg
     1621 tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga
     1681 gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaaccgt cgtaacggat
     1741 tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt
     1801 ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt ttggctgccc
     1861 tgacgttgtc aggaatgtcg caagccatcg ccaaagacga acctttaaaa accagcaacg
     1921 gacacagcaa gccgaaagca aaaaaaagcg gcggcagacg tgtcgttgtt ctcgatccag
     1981 gccacggcgg gattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa aaacatgtgg
     2041 tgctggcaat tgccaagaat gtccgttcca tattgcgtaa tcatggcatt gatgcgcgtt
     2101 taacgcgttc tggcgatacg tttatcccac tgtacgatcg cgtggaaatc gcccataaac
     2161 acggcgcaga cctgtttatg tcaattcacg ccgacggttt taccaacccg aaagccgcag
     2221 gagcatcggt ttttgccctc tctaaccgcg gtgcaagtag cgcaatggcg aaatacctgt
     2281 ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc
     2341 tattgcagca agtgctgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc
     2401 tcggctcgca tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg
     2461 aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct
     2521 cgtttatcac caatccggaa gaagagcggc tgttaggcac ggcggcattt cgccagaaaa
     2581 tcgccacagc gattgccgaa ggcgtgatca gttattttca ctggtttgac aaccagaaag
     2641 cacactcgag aaagcggtaa gtgatgaaac ccgacgcaca ccaggttaaa cagttcctgc
     2701 tcaaccttca ggatacaatt tgtcagcagc tgagcgccgt cgatggcgca gagtttgtcg
     2761 aagatagctg gcagcgcgaa gctggcggcg gcggacgtag ccgggtgttg cgtaatggtg
     2821 gtgttttcga acaggcgggt gtcaactttt cacatgtcca cggtgaggcg atgcctgctt
     2881 ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac
     2941 tggtagtgca tccgcataat ccgtacgttc ccaccagcca cgcgaatgtg cggtttttta
     3001 ttgccgaaaa accgggtgcc gaacccgtct ggtggtttgg cggcggcttc gacttaaccc
     3061 ctttctatgg ttttgaagaa gacgccattc actggcaccg caccgcccgt gacctgtgcc
     3121 tgccatttgg cgaagacgtt tatccgcgtt acaaaaagtg gtgcgacgaa tacttctacc
     3181 tcaaacatcg taacgaacaa cgcggcattg gcggcctgtt ctttgatgac ctgaacacgc
     3241 cagacttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac accaacgctt
     3301 atttaccaat tgtagagcga cgtaaaacga tggcctacgg cgagcgcgag cgcaatttcc
     3361 agttatatcg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgt ggcacactgt
     3421 ttggcctgca aaccggcggg cgcaccgagt ctatcctgat gtcaatgccg ccgctggtac
     3481 gctgggagta cgattatcag ccagaagcag gcagcccgga agcggcgttg agtgagttta
     3541 ttaaggtcag ggattgggtg taactccctc ccccccattc ccgcatccgc tgatgcagcg
     3601 tcagtgacgg cttctcggca aacagctgct gataatccgt ggcgaattgc cccagatgcc
     3661 agaatcccca ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccaa gggcttatca
     3721 gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca cgcatttggg ccaatgccta
     3781 aaatagcgtg aaacgcgttt tgtagcgtgc ggcggctgac atgcagttga ttacacaagt
     3841 ccagcaccgt caccggttcg gacatgtttt ccagcacata ttcacgggcg cgggaaagca
     3901 accgacggta actctgatga ctgatgcttt ccgccgtcac cattggctgc gcttcttcca
     3961 gcatcgcccc catcgccatt agcaaattat cccccagcac tttacgcaca gcaggctgat
     4021 gaagattttc cgggttctcg caaaatgtcg ccagcgcctg ttggacaaag ccccacagcg
     4081 cggctttatg ctgttctttc acttccagcg ccgactggct acgcagcata tgtaataccc
     4141 gatccggatt atgcaaaaag ttagcctgac gggtgatgac atcttcagaa agcactacgc
     4201 ccaggatcgt gtaatcgtcc ggcgtactta attcaaactc ggtgccgcca gggcgcgtcg
     4261 caatttctgc gcttcccaaa cattgcgaac cgataaatcc ctgctcaccg cgcgtcgccg
     4321 gaatgccaaa ccagaacgag ttcggccaga ccaggcacga ctgacgcagc gccagaccgg
     4381 tgtattcacg aaaaacctga atatcatcga gtagaatttc cgtaaattca ccatgaaact
     4441 tgcccggatg cagctgatcg taaatctgct gccaggcggt aatcgtcaaa gcatgttcat
     4501 agacatccgt tgtctgtcgt tgatgaacat tatccacttc gaccttcggc gtgagcttca
     4561 ggttttcggg taagggttca tgataaagat ggtgcaaatt ggctgtacgg gtctttttca
     4621 tgatgttaat gccgggtgtt gtaggacacc cggcacctcc gacaggttaa tggggcttga
     4681 gacgataacg actactgcgt ttacgtaacg tcccggcaga aaagagctgt tcgagcgcat
     4741 ttctggcttt ttccagcggc cagccaaagt gggcagccac ttctcctgcc gtcattccct
     4801 gacgtactga tgttaacagc gccaacaact cgtcagcaga ttccgcgacg ataactggcg
     4861 cgtcgggttc ctttacgggt tgcttaggct ggcggttaaa gccagtaacc agccactggg
     4921 tgtcatcgtc tggccgaccg atctccttgc ggctgatgac acgcccggta cgcatcgcgg
     4981 cagcacaacc tgcgtccagc gccgcacgac acgccgccag atcgccttcc accaccagcg
     5041 ttaggcgacc agggtcaagc acttcgtgac tgagcagacg aacgttagct gctttgagca
     5101 tggcatccgc agcatctagc gcggcgacca tgccgtccac ttccagcaat cccagtgcat
     5161 tgatcatcgg caacctccgt tacgcacgct ggattggatt acgcgcgatt tccagcactg
     5221 catcggtaaa ggcgttacag gctgctttac acgctgcctg gctaccggtt aaaaatgctg
     5281 ccgagtagtt ggtttcagac ggtggcggga cataggtcac cagctgtacg tcggcagatt
     5341 tcagggctgc atcaatgcca taggtcgctt ccagcggcgg tgccaccaga tacgccatcg
     5401 gatcgccaag cgtgatcccg gcggttgatg agagataaga accggtacgc gaaactacat
     5461 gtgccaggaa ggctgtattt tctgcgtcat tagcccactg gaaagcagcg ccattttcaa
     5521 tatgcgcaac catcgcatcc agacctgcac gcacttccgc cgggtttggc ccaccaagca
     5581 taatcagcac ttcaccggca gtcggtgacg gtccgtgtgc agcgcccgca tacagcgagc
     5641 gaccatacac cacttcgacc atcgcctgtt tggtcgcttc gtcagccgca atgtaggtga
     5701 cgtcatcgga atcagcagaa atcagcccga ggctacgaat atgcggcggc aatttcagtt
     5761 cacgcgcaaa ttcggcgtta acagaggcaa tcacccgcat ggcggtgacc gacggtcgaa
     5821 tcaaatctaa agctggcatg atgcctcctt atcgggtcat gttgatgccg gatgctttct
     5881 gctccagcat acgtttcgcc aaatccacaa tgacggctgc ggcttcaacc ggcggcgtgc
     5941 cgccctggtg aatgttagag atgcaggtgc gatcggcctc aacggtggtc gccatacgcg
     6001 gcgagtaaac ggcgtagcag gagagacttt ctgactgccc taatcccggg cgttccccca
     6061 ccagcaggat caccactttc gcgccgagga tctcaccgat ctgatcttca atcttcacgc
     6121 gaccataacg aacaaagaat ggcgtaccga ctttcagccc ggcctgtttc aggcccgcca
     6181 tcagcggtgg caggatctct tcgtagttca cggtgatcgc atcagttgac aggccatcag
     6241 aaataacgac ctgtacgtcc ggattagcaa cacactgtgc tttcagcgct tctacagctt
     6301 ctgcacacag gcggcggccc atatccggac gagtcaggta gagatttttg tcgctaattt
     6361 cagagcggac ttccagcaag ccctgcgctt tcacccactc ttccggcact tctttcagta
     6421 cggtatcttt cgaacgagag tgatcggcca ggaaacgcag cagcgcctgg gtacgcggac
     6481 gcggaccggc acgaccggta caaacgcggg ccacggtgct gcgacgcagt tctgttaata
     6541 cgtctgcgcg atgcgggttt tcaacgccaa tccacgcttt tgcttccgca gagcctaaat
     6601 ccagcgcgca gctttccgag gtcaccggtg ccgcacaggt ggtggtggcg cactttgctt
     6661 ctgacggcgc gggggccgct tgtcccattg acgccatcac gctgcgtaca atttcttcaa
     6721 tctgtttttg atccatgatg tgttatctcc gcgtcatcag aagaacagtg acggatcgcc
     6781 cgcccgtttg gtcaggcgac cgtttgccat aatgcccatg ctttccagcc agcgttcaaa
     6841 ctccggtgac gggcgcaggt tgagtaactg acgcacagtg gcagtgtcgt ggaatgcggt
     6901 ggtctgatag ttgagcatga tgtcatcgcc cagcggcatc cccatgatgt agttgcagcc
     6961 tgcggtggcg agcaggatca tcaggttttc gttgaggttc tggtcagcgt cagcgtggtt
     7021 ggtgtagcag cagtcacagc ccatagagat gccgctcagc ttacccataa agtgatcttc
     7081 taagcccgca cggataatct ggcggtcgtt gtagagatac tccggcccaa taaagccgac
     7141 cacggtgttg acgataaacg gatcgtaatg acgcgccagc ccatagttac gcgcttccat
     7201 cgtcacctgg tctgcgccga agttcgcgcc agcggacagc gcagagcctt gtccggtttc
     7261 gaagtagagg cagttttccc cggcgatacg gttgaactct gcgcccactg cgcgcgcttc
     7321 gtcgagcatc gccagttcca cgccaaactc tttcagccct ttttcgctgc cacagatact
     7381 ctggaaaatc agtccgccag gcgcgccgcg acggatcgct tcaatctggg tggtgacgtg
     7441 cgccagcacg cagccctggg tcggaatgtt gaatttgtcg atcacgccat agatggtgtc
     7501 caacacgcgg cttaagtttt ccacgtcgtc agtcaccggg ttaacgccga tcaccgcatc
     7561 gcccaccccg aaggaaagcc cttcgtagat ttgcgcggcg atactttgca cgtcgtcacg
     7621 ggtgtcgttt ggctgcaagc gggcgctgaa ggtgcccgga ataccgatgg tggtattggc
     7681 ctttttgatc accggcattt tcttcgcgcc gtagatcagg tccgcgttgg agcaaatctt
     7741 cgctaccgcc gcgaccactt ccgaggtcag ccctttgcgg gtaaaggcaa tgtcgtccac
     7801 gctggtttca tcgctcagca catactcacg cagttcgctg atgctccagt ttttaatctg
     7861 gttgtaggcc gtttcgttaa catcgtcctg aatcagccgc gtcacgcagt catcttcata
     7921 ggcaatcacc ggattattgc ggatgtccgc tacggtcatt tccgacaaca cctgctttgc
     7981 cgccacgcgc tcctgtgaac ttgccgctgc aacgcctgcc agcacatccc cagaacgcag
     8041 ttcgttggct ttagccagca cctcttttac atccttaaac tgatatacat tgccgaacaa
     8101 tgtggtcttt agtttcataa gtcgttccct caggaaggaa atgcgagtga tttcaccgtc
     8161 accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt cgatatagtc ccccgcgcgg
     8221 acaatcactt cgtcaatgac tgccaacggg agttgttgta gctgtgggcg caacaacatg
     8281 cccagagctt taccaaagtc ctgcccggcc accaccagca ggggatgcgg attcggaaaa
     8341 cgcgcgacga aatcgaccag cgcgttgatg accgtcagta ccgcggcgta acgcacaggc
     8401 agcgaggcgg gaagcgccag cacgtacgcg tcagaattgg gatcaagatc cagctgaatc
     8461 agcgcctgtt gccaggcact caccagatcc gtttcatcaa ttgggatcgc caccggcaaa
     8521 ttgcgcagcg gcagttgtac gccctccagc cagattgtgc tgccagagag cgaaagggta
     8581 tgtgcacccg cgccaatcac cgtggcgcgt acggtttgcg ccggaaactg cacgttcatc
     8641 tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa gcgggccgat atcggcaaaa
     8701 cagaacgggt cggcgggctg gtggcgataa cattcgccca cgccgccaga aagcgtaatg
     8761 atttcgggcg taacacctgc gggcagcaaa ccggtttgca tcaatgcctg cgcgagcggc
     8821 gagagcgttc cgtcaatcac ttcgacaatc agctctgcca tccggcgggt aacctgcacc
     8881 agctgcgcgc cggtcagcga acgggcgtca gtgcctgcac cgaagcactc atccacaatc
     8941 atctgccccg gtttatgagc gtaaaccacg cgcccctggc tgtcagtttc cagcaggcga
     9001 ccaccgacgt tgaggcaggc agtgccgctg atttttccgg catcgaacag ggcgtagttc
     9061 gcggtgccac cgccgatgtc gatattcagt acccgacaca gccgttgttc agaaagggtt
     9121 tgcgccccgg caccgtgacc ggcgatcacg gattcgagat gcggcccggc gctggcgacg
     9181 acaaaatcgc ccagcgactg ggagagcgtc atcaccgccg ggcgagcgtt gcgggttttc
     9241 gcgctttcac cggtgatgat gatggcacca gaatcaacgc tttccggcgc aatgcccgca
     9301 gcctgatatt gctctaaaat cagcgatttc agttccgctt cttttaaacc accctgttta
     9361 tcgacagggg taaagaacac cgggctttgc cagctaattt cgcgtttgat gaattcgtag
     9421 cgcggcacct gcgacaccgc cgcacggtta accagctcca ggtgggagaa gatcacctgg
     9481 gtggtggtgg tgccgatatc gataccgacg ctcagtagct ggcgggtgtt cacgattgtg
     9541 cctccgcttc ggttttggcc gcggtcgcgt cttcttttgg caccagcatc atcgccacgc
     9601 caatcgccgt tacgccgccg atcaacttgc cgacaatcat cgggaaaatc atggcgttca
     9661 tgttggcagc ggcgaagcct aagtggtcac ccagggcgaa agcagcggaa acggcgaagg
     9721 cgcagttgat gactttgccg cgggtatcca tctgcttcat catgccaaac attgggatgt
     9781 tgttggcaag cgttgccacc atgccagctg ccgcgatatt gttcatattc agcactttac
     9841 cgacgctcat cagcggtttt tcaaaccagc gggtcagcag caataccatc gggtacgccc
     9901 ctaacagaac acaggagata gaaccgataa cttcaatggc gcgcatcact tcaccgggtt
     9961 tgtcgcctgg agccatgaag atcggatcca gaccggggat cagttcccaa ccgagcagga
    10021 atttcactac cgcagcggca agaccgaggg tgatcaatgc aacgaggaat ttggcgaaga
    10081 tctggaagcc gttgatcatt ttttccggga tgaatttcag ccccagcgcc accagtaccg
    10141 cgacgataag taccgggatc atgttcatca ggatcagggc aaaggtgaat tccactggct
    10201 gaccgttgat ctgcacaccg gaatacatag ccaccagacc accggcaata cagccaatcg
    10261 gaatggtcac aatgcccgcc agcacgccga gcgccagata acgacggtca gaaggttcga
    10321 taatgccgag cgccaccgga atggaaaaca caatcgttgg ccccatcatc gacccgagaa
    10381 ttaacccaga gtatagccac gctgctacgt cgccgcccgc cagctctttg gcgaggaaga
    10441 agccgcccat atcgcacgcc agcagtgttc cggcgaacat tgacgggttt gcgccgagca
    10501 tttcgtaaac cgggataatc accggtccga gtacgtgggc cagtaccggt gccagcgcgg
    10561 tcataccgac catcgccagg cccagtgcgc ccattgccat aaagccttct tcgaactgac
    10621 cgccggatcc ttcgatactt ttaccgaact taccgaggaa acgagcagaa ccgccgaact
    10681 gcgacaggat cctgtctacg gcagctatca gcataaagaa catcatgatg tacatgatga
    10741 tttcgttaat tcccatcgcc tttactccct gttagttgtt atttattgcc aatgctgaaa
    10801 gtgggttttt ccctcactct ggaagccgag gggccggggt gagggtaatt gtttgcaccg
    10861 gcgcgactct gttcccctca ccctaaccct ctccccaaag gggcgagggg actgtccgta
    10921 cacttttatt gcgccgctgc gtacaagccg acaatctgct ccaggctggc ggtacgcggg
    10981 ttgctgcgca gacaaatatc ttccagcgcg gcctgcgccc atgcgccgta atgcgcagat
    11041 gttgcaccaa catcgcccag tcgtttacca atcccgactt ccgcaatcag ctcacttacc
    11101 gcgttaatag cgtcacgatc gtcggatttt ttagttcgca gtgcccgacc aatctgacta
    11161 aaccgatcac gacaaaccat ccggttaaat tccatcaccg ttggcagcaa catggcgttc
    11221 gccagaccgt gcggaatatg cagcgccgcc cctggctgat gcgccatcgc gtggcacaac
    11281 ccaagccccg cactggaaaa cgccattccc gccatacatg aagccagcaa catgctctcg
    11341 cgggcggcaa ggtcgtgacc gtagcccacc gctttcggca gcgatttgcc aatcatcgca
    11401 atggcaccaa tcgccagact gtcggtaaac ggtgtggcgt tcagcgcgct gtacgcttca
    11461 atagcatggg ttaacgcgtc gataccggtc atcgccgtga catgcgacgg cacgccttcg
    11521 gtcagcgcag cgtcgagaat cgccacatcc ggcatcagcg aggcgtgggc taacacctgc
    11581 ttgcgcccgc tcaccgcgtc gataatcacc gttacgttgg tggtttcaga accggttccg
    11641 gcggtagttg gaatggcaat caacggcagg cgcggttgca gaacgctggt ttctgacatc
    11701 tctgccagcg tgctatccgg gttcgtcacc agcaacgcca cggcttttgc tgcatccagc
    11761 accgagccgc cgccaaacgc aatcaccccg tcacagcctg attcacgcaa ctgcgccacg
    11821 gctgcacaca cgtcggtgat gcatggttcg cccaccggac atggccagag cgtcatggcg
    11881 atacctttaa cggccaggct acgcgtcagc ccggcggtca tccctgcctg atgcaaaaag
    11941 ctatctgcca tcacgaacag atgtttcagc ccacgcgttt gcgcttgctg tccgcaactg
    12001 ctcaccgcgc ccggaccgca aagcgtcacc ggtggaacgc taaatgtttt tacccgttgc
    12061 agattcaggg tatcgaacgc ctgaaagagc gcggtctgca attcattttg catagatccc
    12121 ctccgctttc tctcgcccgc tactggcgat cgccagcggg gtcataaaca aactgtgctg
    12181 cggtagatgc acctgtaacg ccgggaattg tttgcgaaac agttccgcca cgcccggttg
    12241 catacaagag ccgcccgcca gccataaatc agtaatgccc tgcccttcaa tatgccgggc
    12301 gacaatgtcc gccatttttt cgtacaccgg tttcaccgca ggccaaatct cgtcaccgtg
    12361 accgcgtttg tactgctctg cctcttccag cgaaatacgg cgatttccgg cgagagtcag
    12421 agagatatga tgtccgccgg tggcttcatc tgccgagtaa gtcaccttgc cctttttcac
    12481 gatggcaata ccagtagtgc caccgccgat atccactaca ccggcgttgt ccagttgcag
    12541 cagatccgcg actgcggttg gctcatccag cacatgactc acttccagtc cggcagactc
    12601 cagcacgttg atggaaatac gcgggtccgt acctggagga aaagaggtcg ccgcatggct
    12661 aaaacggcga ccgaattgct gctcgagggt gtcgagatgg cgacgaacaa tggtgaccgc
    12721 accgaagaaa tcccagacga tgccgtcgcg gaccacatca gcccagtcga ggcataccgc
    12781 caccggctga ccgtcgcggt caacaaccat cgacaccaca tcgcaggtgc ccagatccac
    12841 ccccagccac agcggagatt ccgtcgcggc aggcgtctgg ttacacagcg ttgctgctat
    12901 gtgcagacgt ggggtgagcc attgttcgtc gtgcgccatc tgttactcct taaacaatgc
    12961 gaaacgcatc gactaataca cagcgacgca gacggacaaa cgtccgcgcg ctggttaccc
    13021 cttcaccggt tggcgtggtg atggtcatgg tggtccagcc ttccccgccc agccccagcc
    13081 cggcaataca cggtccgttc ttaacgaaaa tgctggtatc aatggcattc gccatctggt
    13141 tcatgttttc gatgttgcgc gagtgcattg ccgccgtgtg atggcaaccg ccttccagtt
    13201 tcaccgccag cgcaatggca tccgccacgt tggcgacgcg cacgacgggc agcaccggca
    13261 tcatcagttc agtcacagca aacggatgtt ctgcggtggt ttctacaaac agcagtcgcg
    13321 tttgttccgg aactttaagg ccgattgccg ccgcgatttt cgccgcatcg cgaccaaccc
    13381 agtcacggtt gacggtgcct tttccgcgct cgtcgatatt tttcagcaac accggttgta
    13441 gctgctgcgc ctgctctgcg gtcagtttca ccgcgtgctg gccttccatc agacgcatca
    13501 gttcatcggc tacgctatca acaacaatca gtaccttttc gtcggcacaa atgatgttgt
    13561 tatcgaaaga agcacctttg acgatggact gagctgcacg ggcgaggtca gcggtttcat
    13621 ccaccactac cggcgggttg ccagcacctg cggcaatcag acgtttattg gtgtgtttac
    13681 gcgccgcttc cactaccgct tcgccgccgg tcaccaccag caggccgata cccgggaact
    13741 tgaacaaacg ttgcgcgatt tcgatatccg gattggccac agtaaccagt aagttttccg
    13801 gcccgcctgc ggcaacaatc gcctggttga gcagcgtgat cgcccgctgg gagacttttt
    13861 tcgccgccgg atgcggggca aaaatgacgc tgttgcccgc ggcaatcagg ctgatggcgt
    13921 tgttaattac ggttgccgcc gggttagtgg aaggcgtcac cgaagccacc acgccccagg
    13981 gcgcgttttc aattaaagtc aggccgttgt caccggtcag cacctgtggt gagaggcact
    14041 caacgcctgg tgtgccgcgc gcctgagcga cgtttttggc aaatttatct tcgacgcggc
    14101 ccatgccggt ttcactgacg gcaagttccg ctaaatctct ggcgtgtttt tcgcctgctt
    14161 cacgaatggc agcaatggct aactggcgca ttgccacgct ttttaaccct tgctgggcaa
    14221 ttttggctgc cgcaacggcg tcatccaggg acgcgaaaac gcccatctca tgaacggcgg
    14281 cgggcggcgt gtcactgctt tgcattttca gcagtaccgc tttcaccacc tgttcaatat
    14341 cctgttgatt catgatgttc tgccttattt gtggaaaatt acctgaccgc cagacaccac
    14401 ctcatcgacg atgccaatca cgcacagatc gaccggtgac gtttcgcttt tatgcgcctg
    14461 gcgggcagaa ctgccactca ccagcaacac ccactccccg gttcccgcgc caatattgtc
    14521 gatggcgacg gcgcattgcc cgtcaggatt accttgtgga tcaatcattt ccaccatcag
    14581 caatttgtca tgcgccagtc cgtgatggcg tacggtacaa acaatttgtc cagtgacgac
    14641 tgccagtttc atacccgcct ccgtggcgta tttcaggtaa aagctccccc taccttccgc
    14701 ggaaggtaaa atgaaaaagg agagagcgtg acgcccgaat cgacgtcaca cagggtgatt
    14761 acaggttgct gctatcgcct ttcaggccga tcgggaagac ttcttccagg tcgccgtgcg
    14821 gacgcgggat aacgtgtaca gataccagct cgccgatacg ctgtgccgct gcagcacctg
    14881 cgtcggtcgc ggctttacag gctgcaacat caccacgaac catggcagta cacaggccgc
    14941 cgccaatctg cttaacacca accagcttga cgcgcgcagc tttcaccatc gcgtcagaag
    15001 cctcaatcag tgcaaccagg ccccgggttt cgatcattcc taatgcttcc attgtgcttt
    15061 cctctttatc agggtccaga acgggaccgt tcattcaacc agtgtttgta aactgctttc
    15121 gcggttcact tctgtctgac gcggcacagc tgccaccagc gccagctcga taatttcctg
    15181 cacgctacaa ccacgagaga gatcgtgcat cggcgcggca agtccttgta tcaatggccc
    15241 gacggcacga tatccgccga gtcgttgtgc gattttgtaa ccaatatttc cggcttccag
    15301 cgacggaaaa accatcacat tggccttgcc ttgtagcggg ctggcaggcg ctttttgcgc
    15361 cgccacttcc ggcacgaagg cggcgtcaaa ctgcaactcg ccatcgacta ccaactgcgg
    15421 cgcgcgctca cggacgattt ctgtcgcctg ctggacgttg gcaacgcagg ggtgacgggc
    15481 gctgccgtta ctggaaaacg acaacatcgc cacgcgcggt tcttctccgg tgatggcgcg
    15541 ccaggtttcg gcgctggcaa gcgcgatatc cgccagttgc gccgccgtcg gctgtggcac
    15601 tacgctgcaa tcggcaaagc ccaatgcggg gccactgtac tgtggcagca tcaggaaaat
    15661 ggaagagagc gttttacagc caggctgcaa gccgataatg cgtagtccgg cacgcagcac
    15721 attcgccgtg gaagagagat tgcccgcgat acagacatcc gctttaccgg cgctgaccat
    15781 tgctgcggcg aacatcagcg ggtcggtaag tttttccagc gcatccggcg gcgttttttc
    15841 gcccgcgcgg gccagccaga gatgagcaaa ttcttcccgc attgcgaggt tgccatgcgg
    15901 atcaatcacc tgtagcccgt ccatcgctac gccatgactg agcgtaaact gacgaagttc
    15961 aaacggatta gcgaccagaa tgggcgttgc cagaccttgt tgatgtaaat attgcgcagc
    16021 tttcagcaca cgctgatcta acgcatccgg aaaaaccact ctggcgggcg ctcgcaacgc
    16081 cagttcacga caacgttcaa taatcattgc gtttccccca gtcgttgttg gatctgcttc
    16141 accgttagcg gcggcttcgt tacgctcagg atcatcatca cgtagaccgt gctggaaagg
    16201 cggttcagcg cctgcaagat gtccgggcgt aatacttcaa agctgcgggt gataaacacc
    16261 tgcgccgcta cggtttcggt ttcccgcact ttggtgcgta gcagattcag caacgccgca
    16321 tcgcgcccgt ggctggcttc cggcaccaga tgatcgtgac caagatagcg caacggctgg
    16381 tgagaaagcc gatgcagatc ttcgtcagaa agcccgacaa tcgcttggca ggccagcggt
    16441 tcccccagcg catcggcgcg cataatgttg ccaagacgcg agcggatgtc cgccagccat
    16501 ggctgccacg gttctgccag ttcaatttgc agccacactg ccagcgcaat ggtgctgtcg
    16561 agaacagcgc gaaaacccag gcgcggatcg cttttggcga ccattttttc cgccgacagg
    16621 tgagtcagcg tatccggctt tttcgccacc ggctggcggc acagttcgca gcacgcctgc
    16681 ggatgttcat cgctactggt cagcccgtga acgggctgtg gctgctgctg ttcatcgtca
    16741 acaaacaggc ggccctgctc gtcaataaac ttgatgcgca gatggcggct ttccagcaac
    16801 tcccgggcag agggcgtcag gcgactgtcc gcagggagat ggatctctgc gccttcgctg
    16861 agcgtatggt tcgctcttag ccatgcttcg gtgatgaaat ctttcatagg gattgccagt
    16921 tagccggcca ggcgacatac aggaatttca cgctggatgt cgtaccaaat tcgatgctgg
    16981 agcctttcgg gataaacatc acgtcgcctg ctttggcgat catggtttcg ccttcgtggc
    17041 gaacgtgcag ttccccttcc agtaccatgt cgatttcgtc atagttcagg gtccacggga
    17101 agaaggcgtt ctcccactgc ataaaaccag cggccatgct gctgccgtcg tctccggtga
    17161 ccagatccgt taatccgacg cagtgtggct gtgcgccgtc aaaacgacca aacttgacgc
    17221 tgctgccatc aataactttg atgccgcctt tgccggtaac cgatttgaaa ctcggctgca
    17281 ttgcaccctg ctccagtgac tgtttttcct tcatcacttt ttccatcagc tgcgcgacca
    17341 ggctttcggt aaactggcct tccggcagct gggcaatgat ggtttcgcgg atacgctggc
    17401 tttcggtttt atcggctggc acgctggcgg gcacggacgc cgttaccggg atcgattcat
    17461 cacattcggt gatggtaaaa cccagtagat ccgccacttc acgggcttcc ggagtaataa
    17521 tgctggcgcg cagaaccacg gacattgcct gttcgccgcg cgcgtgtgct tcacgaatat
    17581 cattcgctgt gatgagtttt ttcacctgct tcctcctgtt tggtgagaga tgccagataa
    17641 tccaccagtt gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat cggctcttcg
    17701 aagcccgtct caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg catgtccgtt
    17761 ttactgatga cggcgatttg ccgtttgctg acgccaatat ccagcaaccc ggcaggcaac
    17821 cgactttccg ggtcattcgc gccgtggaca tagatcagca tgtccacatc ctgtagcgtg
    17881 gtaattaagg cgtgatacca gcggggatgg ctaaaatatt cacccggcgt atcaatatca
    17941 cccttatcat taaattctac ggcctgcgtt tttctggcga gggtataatc tccctgcaac
    18001 gcattaaata acgttgtttt tcctgccccg acggtaccca caaacgcaat tcgtttcatg
    18061 aatgcctcgt attaactttt ggtcatttca caaagcgtat aatttaataa acgccctaaa
    18121 ccgctgacgg tttgtgataa cgcttcttct actgcaccca ccgagccata aatcaccaac
    18181 gcgccgctaa aacggtcgag aaaaccgata tgcacatcgg cggcttttag tgctaaatca
    18241 ccggcgatca tcgccgtttc gcctggagtt agcgtcataa tgccgattgc gcccgcatca
    18301 ggaacgccga ttttcttcgc cagttcctcg ccgggatgcg cgatgagatg cgccagtgtg
    18361 acctgtttac cgggcacaaa ttcctgaatg atgcgttctt tatccatgag tcgccgcctg
    18421 tgaaaaactt tcgtgtatcg tgacaatttt tcgtcagagt aaataacaaa attcggcaag
    18481 cgcgtttaaa aggtgaggta gatcactaag aatggagaga
//
LOCUS       DARNVM010000094        18410 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.094, whole genome shotgun sequence.
ACCESSION   DARNVM010000094 DARNVM010000000
VERSION     DARNVM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.094"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..210)
                     /gene="cedA"
                     /locus_tag="SI621_002911"
     CDS             complement(<1..210)
                     /gene="cedA"
                     /locus_tag="SI621_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HEI4065601.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYIPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPE"
     gene            393..2654
                     /gene="katE"
                     /locus_tag="SI621_002912"
     CDS             393..2654
                     /gene="katE"
                     /locus_tag="SI621_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HEI4065602.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPTAEPTP
                     PGAQPTTPGSLKAPDTRNEKLNSLEDVRKGSENFALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     MWSRIPKIDKIPA"
     gene            complement(2701..3450)
                     /gene="chbG"
                     /locus_tag="SI621_002913"
     CDS             complement(2701..3450)
                     /gene="chbG"
                     /locus_tag="SI621_002913"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HEI4065603.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRADRQMVFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSAYCL
                     PRLTELDVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3463..4815)
                     /gene="celF"
                     /locus_tag="SI621_002914"
                     /gene_synonym="chbF"
     CDS             complement(3463..4815)
                     /gene="celF"
                     /locus_tag="SI621_002914"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEI4065604.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(4919..5761)
                     /gene="chbR"
                     /locus_tag="SI621_002915"
     CDS             complement(4919..5761)
                     /gene="chbR"
                     /locus_tag="SI621_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HEI4065605.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5769..6119)
                     /gene="chbA"
                     /locus_tag="SI621_002916"
     CDS             complement(5769..6119)
                     /gene="chbA"
                     /locus_tag="SI621_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HEI4065606.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6170..7528)
                     /gene="chbC"
                     /locus_tag="SI621_002917"
     CDS             complement(6170..7528)
                     /gene="chbC"
                     /locus_tag="SI621_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HEI4065607.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7613..7933)
                     /gene="chbB"
                     /locus_tag="SI621_002918"
     CDS             complement(7613..7933)
                     /gene="chbB"
                     /locus_tag="SI621_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HEI4065608.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8232..8570)
                     /gene="osmE"
                     /locus_tag="SI621_002919"
     CDS             complement(8232..8570)
                     /gene="osmE"
                     /locus_tag="SI621_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HEI4065609.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8772..9599
                     /gene="nadE"
                     /locus_tag="SI621_002920"
     CDS             8772..9599
                     /gene="nadE"
                     /locus_tag="SI621_002920"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEI4065610.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9829..10716
                     /gene="cho"
                     /locus_tag="SI621_002921"
     CDS             9829..10716
                     /gene="cho"
                     /locus_tag="SI621_002921"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HEI4065611.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10676..11251)
                     /gene="ves"
                     /locus_tag="SI621_002922"
     CDS             complement(10676..11251)
                     /gene="ves"
                     /locus_tag="SI621_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HEI4065612.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11454..11939)
                     /gene="spy"
                     /locus_tag="SI621_002923"
     CDS             complement(11454..11939)
                     /gene="spy"
                     /locus_tag="SI621_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HEI4065613.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12269..13237)
                     /gene="astE"
                     /locus_tag="SI621_002924"
     CDS             complement(12269..13237)
                     /gene="astE"
                     /locus_tag="SI621_002924"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEI4065614.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQTPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13230..14573)
                     /gene="astB"
                     /locus_tag="SI621_002925"
     CDS             complement(13230..14573)
                     /gene="astB"
                     /locus_tag="SI621_002925"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEI4065615.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTSHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATVAPSTDTLDGKVHLTVANLNNKFHRSLEAPVSESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQVQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14570..16048)
                     /gene="astD"
                     /locus_tag="SI621_002926"
     CDS             complement(14570..16048)
                     /gene="astD"
                     /locus_tag="SI621_002926"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEI4065616.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGKWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGRRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPAREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16045..17079)
                     /gene="astA"
                     /locus_tag="SI621_002927"
     CDS             complement(16045..17079)
                     /gene="astA"
                     /locus_tag="SI621_002927"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEI4065617.1"
                     /translation="MMVIRPVERSDISALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17076..18296)
                     /gene="astC"
                     /locus_tag="SI621_002928"
     CDS             complement(17076..18296)
                     /gene="astC"
                     /locus_tag="SI621_002928"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HEI4065618.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLTDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDILHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLHGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
BASE COUNT         4654 a         4959 c         4371 g         4426 t
ORIGIN      
        1 ttcaggcaca gtaaatgccg gtgagcgcag aaatgactct cccatcagta caaacgcaac
       61 atatttgcca cgcagcatcc agacatcacg aaacgaatcc atctttatcg catgttctgg
      121 cggcgcgggt tccgtgcgtg ggatatagct aataatctgg cggttttgct gacggagcgg
      181 tttcttcatt actggcttca ctaaacgcat attaaaaatc agaaaaactg tagtttagcc
      241 gatttagccc ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtc
      301 tccgaagcgg gatctggctg atggtctata gttagagggt tttttgacca aaacagcggc
      361 cctttcagta ataagttaag gagacgagtt caatgtcgca acataacgaa aagaatccac
      421 atcagcacca gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg
      481 cacctgaaga cggatctcat cgtccaacgg ctgaaccaac accgccaggc gcacaaccta
      541 ccacccctgg gagcctgaaa gcccctgata cgcgtaacga aaaacttaat tctctggaag
      601 acgtacgcaa aggcagtgaa aattttgcgc tgaccactaa tcagggcgtg cgcatcgccg
      661 atgatcaaaa ctcactgcgt gccggtagcc gtggtccaac gctgctggaa gattttattc
      721 tgcgcgagaa aatcacccac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac
      781 gcggatcagc cgctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag
      841 cggatttcct ctcagatccg aacaaaatca ccccagtatt tgttaggttc tctaccgttc
      901 agggtggtgc tggctctgcc gataccgtgc gtgatatccg tggctttgcc accaagttct
      961 ataccgaaga gggtattttt gacctcgttg gcaataacac gccaatcttc tttatccagg
     1021 atgcgcataa attccccgat tttgttcatg cggtaaaacc agaaccgcac tgggcaattc
     1081 cacaagggca aagcgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc
     1141 tgcacaacgt gatgtgggcg atgtcggatc gcggtatccc gcgcagttac cgcaccatgg
     1201 aaggcttcgg tattcacacc ttccgcctga ttaatgccga agggaaggca acgttcgtac
     1261 gtttccactg gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac
     1321 taaccggacg tgacccggac ttccaccgcc gcgagttgtg ggaagccatc gaagcaggcg
     1381 attttccgga atacgaactg ggcttccagt tgattcctga ggaagatgaa tttaagttcg
     1441 acttcgacct tcttgatcca actaaactta tcccggaaga actggtgccc gttcagcgtg
     1501 tcggcaaaat ggtgctcaat cgcaatccgg ataacttctt tgccgaaaac gaacaagcag
     1561 cattccatcc aggtcatatt gttcctggtc tggacttcac caacgatccc ctgttgcagg
     1621 gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg
     1681 agattccgat taaccgcccg acctgccctt accataattt ccagcgtgac ggcatgcatc
     1741 gtatggggat cgacactaac ccggcgaatt atgaaccgaa ctcgatcaac gataactggc
     1801 cgcgcgaaac accgccgggg ccgaaacgcg gcggttttga atcataccag gagcgcgtgg
     1861 aaggcaataa agttcgcgag cgcagcccat cgtttggcga atattattcc catccgcgtc
     1921 tgttctggct aagtcagacg ccattcgagc agcgccatat tgtcgatggt ttcagttttg
     1981 agttaagcaa agtagttcgt ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata
     2041 ttgatctcac tctggcccag gcggtggcga aaaatctcgg tatcgagctg acggacgacc
     2101 agctgaatat caccccgcct ccggacgtca acggtctgaa aaaggatcca tccttaagtt
     2161 tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg
     2221 aagtgagatc ggcagacctt ctggccattc tcaaggcgct gaaggccaaa ggcgttcatg
     2281 ccaaactgct ctactcccga atgggtgaag tgactgcgga tgacggtacg gtgttgccta
     2341 tagccgctac ctttgccggt gcaccttcgc tgacggtcga tgcggttatt gtcccttgcg
     2401 gcaatatcgc ggatatcgct gacaacggcg atgccaacta ctacctgatg gaagcctaca
     2461 aacaccttaa accgattgcg ctggcgggag acgcgcgcaa gtttaaagca acaatcaagg
     2521 tcgctgacca gggtgaagaa gggattgtgg aagctgacag cgccgatggt agttttatgg
     2581 atgaactgtt aacgctgatg gcagcacacc gcatgtggtc acgcattcct aagattgaca
     2641 aaattccggc gtaaatatgg ataaggtcac gccccctggg gagtgacctt accaccttac
     2701 ttacacatca agataactac ccaaccgata accacgctca gcaatcgcat atttcaacga
     2761 cgctgaagtc agcacatcca gttccgttaa ccgaggtaag caataagcac tctggcgaat
     2821 cgtattatcg ataaacgccg gatgacacat cacttccaga gagcgctcgc cgcgatgact
     2881 ggagtcatca agcacttgca ggaacagcga ctcactgatc tcttcaccgt agaatgcact
     2941 gctgaatccc tgggtagtgc gcaggttgac cggcaaatca aacaccatct gacgatccgc
     3001 ccgcaacgca atgccctctt ctgccgcaaa cctggcaaca atcgggaaaa tctgcgggaa
     3061 catatgcaca tgatgatggc tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa
     3121 acgcaaatac tgactggcaa gctcctgagt aatttcttcc agcggtaaag catcttcttc
     3181 tgccaactgc cagatccatt ttcccagcac accatcgcgg gttaaccccg gcatagctgt
     3241 cagtggcttg cccatagtaa ggacaaagtg catccctatg gccagactcg gttcatcacg
     3301 actcaactgc accgcatggt caatagcctg cccattcacc agcgccgtcg tcgatgtgac
     3361 aatcccattg cgacaggcct cgataatgcc gtagttctga cctttgctta agccaaaatc
     3421 atcggcatta acaatcagta agcgttccat aatcagcctc ggttaatgtg cttttttaag
     3481 ctctgcgatg cagtcggcaa aatttggcag ccatttctcg tgcgccagaa tcatctcgcg
     3541 tgccagcagc tcagcatcgc gatcggaatg caccaacgga ctaaggttta gcgccagtaa
     3601 cacatcgtta aattctccgc taagtgcagc gttgctggca gcaatctcga agcctttaat
     3661 ggtgtgaatc agccccatca ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc
     3721 gccatcgcgc cccagcgtac aggtcatttc taccgcccag tctgccggaa tattatcaat
     3781 atgcccatga tgcgggatat taacgtaatg ttcagcttgc ttgtcgttgt agatagcgtt
     3841 gatcacttcg cacgctgcat cagagtaata agccccaccg cgctgttcca gttctttcgg
     3901 cttaactttc aactccgggt ttttatacag ctcaaaaagt tgtttctcga ctttctgtac
     3961 tacctgtgct cgtgcgccgc ctttgtagta ttcgcccatt tcaatagcca gcatctcttt
     4021 ttgcttgaag taataaagca aataagaaca cggcaacaga ttcagagaac gaattaagcc
     4081 ttcgctaaat ggcagatcga aaatattttt aacgccagat gcttttaact gcccggatgc
     4141 cacaccatca agcaattcgg caaagcgtga tttgccattt accagcacat ctttaatgaa
     4201 caccatatgg ttgaggccga acagatcgat agataaatca tcgctgtctt tcagcatcag
     4261 aacatcgcga ataaacatct tcatgccgat cggaatatta cacacgccga taaagcgttt
     4321 aaatccggta tgacgataaa cggcttcagt gaccattccc gccgggttag tgaagttaat
     4381 cacccatgca ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa tcaccggaat
     4441 ggtacgcaga cctttaaaaa gaccgcccgc gccgttggtt tcctgaccaa gataaccatg
     4501 acttaatgga atacgttcat ccagttcgcg cgccggtaat tggcctacgc gcagttgggt
     4561 agtaacgaaa tcagcatctt tcaatgcttc gcggcgatcc agcgttttat aaagcttcat
     4621 cgggacgcca gcgttatcaa tcatccgttg gcagagatca aaaataatat ccagtttagc
     4681 tttaccacct tcgacatcca ccagccataa ttcgctgacc ggcaattcgt gataacgctt
     4741 aataaatcct tccagtaact ccggggtata gctgctcccg ccaccaatag tgacgacttt
     4801 taatttctgg ctcataattt ctcccttcag tacagaatac tgatatctgg catatctgcc
     4861 ccccggacat aaataatcca gcaacaggac agatatgtga attgtcaggt ataacgactt
     4921 actgattaaa ttcagtcaat ttcttacgat agctcttagg cgtaaaggac gttaatttct
     4981 taaacgtttt aataaacaag ctcgggctac tataaccggc ctcaaacgca atatccgtca
     5041 cggaatagtt ggtcatttcc agttgttttt tggcaaaatt aatacggatc tcattaataa
     5101 tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt
     5161 tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca
     5221 tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt
     5281 aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg
     5341 tttcgcggaa attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg
     5401 tccggtatac ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa
     5461 aaaagcgttt actgatccca acattcaata tgcgcgtggc accaaactca taaaaacttt
     5521 gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac
     5581 cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct
     5641 gatgcagtcc gctgatactc tcagttttgt tgtagataaa cacatggaag tttttgccat
     5701 taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca
     5761 tcgactcctt atgccttcag tttttcatga agctcaatta attcggtaat cagttcacgc
     5821 gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact cactttcatc
     5881 ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat
     5941 gccatccgtg actgatccat catggctttt gctgcggcaa aatcgccctg cttcgcctgt
     6001 ttcagtgcgg catacgccag gctgcgcgct tgtccggagt tgatgataag tcccatcacc
     6061 acttcttcca gctcttcagc ttctgtttgc gtatcgggaa tgttatcgag atccatcata
     6121 catcgttcct ctcttttctt accggcacga ttacccgtac cggcatcgat taaaatttca
     6181 gggcgttagc gatatcttct tcgctctctt ctttatcaat cgcgttttgt gctttgttag
     6241 cgaccacaac aaagggcaga taaattaacg ttgcgatacc aaggttgaag agtgcgacca
     6301 gcaatgcggc gacgctaccg ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc
     6361 acggtgcaat attggtcacc ggaggaataa tgcccatgta gtaagctgcg agggtgattg
     6421 ccgccagaat cggttgtacc agtacaaacg gaataaacat caccgggttc atgataattg
     6481 gcagaccaaa cagaatcggt tcgttaatct ggaagatgcc ggacggcagc gccagttttg
     6541 ccacctgacg ataatcagca cggcgagaag cgataaagat agccaggatc agacccaaag
     6601 tcgcaccact gccaccgagg aaaataaagg agtccagcat cggcttggcc cagatatgga
     6661 aggtcttacc ggctgccagc gccgcttcga cggaaccata ttgctgatag gtcgcgatgt
     6721 tttccagcgc ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat
     6781 gaataccgaa gaaccagagc agtggaacaa agatcacata ggcccatccc accacgctac
     6841 ccaacgatgc cagtggggtt gagatggtat ccataatgat ctgatggaaa ttggtacccc
     6901 aggtattcaa cgcccaggca ataatcccca tcacggaaag aataataaag ccggggatta
     6961 atgccgagaa ggaacgcgat actgaagcag gtacgctgtc gggcaattta atgacccaat
     7021 tgcggtggac aataaaggta aacatttctg ccaccaccag gccaataata atcccggaga
     7081 tgatattcgc cccacctaac cagtttgcgc caaccgcata ggcctcaccg acactgtatg
     7141 gggtgacggt cataaatgct gcaacggata acaacccagc cgccagcgca tcgactttac
     7201 gctcttctgc cagcgccatg ccaataaaga acggtgccat taaagacatt atccctaatg
     7261 ttccgttata tacgttgccg ccaatacctt tcagaccatt aagtgtttca atggttgagg
     7321 catccaggcg aatacctaag gaataaaaaa acgacccctc cccaaagctt agaaaaacgt
     7381 tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga ataaagccat
     7441 ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg aggagtacct
     7501 tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg caatttaaat
     7561 attgcggttt tgatttatga aataactctt tgacgggaaa atttaaaata aattaatttg
     7621 ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca tcgactttgc
     7681 cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc tggatttcgg
     7741 gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga cctttttcac
     7801 cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt tctgcctgtg
     7861 cgcgcatttt tgataccagt aaagaggtag acatgcccgc agaacaaaac agataaatgt
     7921 gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac actccgcaag
     7981 ccttaacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt ttttgtggct
     8041 gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat aaattgtctc
     8101 tccttgacct gcggttatga ccctcttcac atttcgggca aataattcgc gaagataatt
     8161 aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgtaac tcaagctttc
     8221 aggcgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca ggtctgataa
     8281 ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc tgctttacca
     8341 tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat gctcacttca
     8401 gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct
     8461 ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca acccgccagc
     8521 atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat attccgtcct
     8581 cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc aagcccgtga
     8641 acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca ttcaacccgc
     8701 tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc ttttctgtct
     8761 ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca aaaccgcaga
     8821 ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt
     8881 atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc acgcttgccg
     8941 gaaagctgtg ccagatggcg attaatgagc tgcgccagga aaccggcaac gaatcgctgc
     9001 aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat tgccaggatg
     9061 ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc gcggtattgg
     9121 ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtt cgtggcaatg
     9181 aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc agcggtgtcg
     9241 tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa tatggtgacg
     9301 gcggtacgga cattaatcca ctgtatcgtc tcaacaaacg tcagggtaaa cagttactgg
     9361 cagcattagg ttgcccggaa cacctttata agaaagcccc aactgccgat ctggaagatg
     9421 atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat atcgacgact
     9481 atctggaagg gaaaaacgta cctgaacagg tcgccagaac aatagagaac tggtatctga
     9541 aaactgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg aaaaagtaat
     9601 aatttgcaca tattggtttg tgcgaaaaag agtactttgt tcacgccgga tgcggcgtga
     9661 acgccttatt cgccccacaa aactatgcaa attcaatata ttgcatgagt cgaataggcc
     9721 tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc ttttttaatc
     9781 gctccccgcc tgttacactg gatagataac cagcattcgg agtcaacagt ggtacggcgt
     9841 ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc cgaacattta
     9901 cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca tggcgaaagt
     9961 gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt gctttctcat
    10021 ttacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag ctggatctgc
    10081 accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga acaacaaccg
    10141 ctgtttaata aacggttgcg ccgcaatcgc cagctctgcg ccctgcaatt aaatgaaaag
    10201 cgtgttgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa cctgtttggc
    10261 ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga tgaacaaaaa
    10321 ctttgctatg gcctgctggg actggaaccg ttaagccgcg ggcgtgcttg ttttcgttct
    10381 gcattaaaac gttgcgctgg ggcgtgttgc ggtaaagaga gccatgagga acatgcgcta
    10441 cgtttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca aggggcggtg
    10501 gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa ctggctgtgg
    10561 ctgggagcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc cgccgggttt
    10621 gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta tgaaattact
    10681 gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac agtttaccct
    10741 gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag
    10801 tgagcagttt gtcgcccagt tgccacgcgc cgttaattac aaacaccacc ccgccgcgtg
    10861 agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag acatccaggc
    10921 gtgtcatgat gttgaaatcc atcgacatct gccctgccgt cagtttcgct tttaccacct
    10981 ggtccgccgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag cggtctgcgc
    11041 tttcaaggaa catctcgccg ccttccagca acgtcactat cctttccata cctggaaaca
    11101 aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa aaatcacgtt
    11161 ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc cacagattca
    11221 ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc ttttcattgt
    11281 tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt tagtggcgat
    11341 caggcttttt ttgcatctgt ttgagaacaa aaaagaaaac cgccgatcct gtccaccgca
    11401 ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa agattattca
    11461 gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt ctcaaaatta
    11521 gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc
    11581 atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg cgcttcagct
    11641 tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg ttcttccagc
    11701 ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg ctgtttctgc
    11761 gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa cttgcctttg
    11821 tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc ggcatgggcc
    11881 aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa tttacgcata
    11941 ttctatatcc ttcctttcag ttatttatta cggctttctt aagaagcgtg ccgtgttgac
    12001 gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa caatgaaagt
    12061 gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg agagtgatga
    12121 gggaatatta caacacgatg atttagcaga gattatgaag aactataccg gatgactggt
    12181 gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag taacttaata
    12241 cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt aatcccgcgc
    12301 gtaatcccaa cgctaccaaa ggattaggga ataacacata ctctacatca tgggttacgg
    12361 taaaacgttc ctccccgtcc tgcgccagca atgttccttt ctcaaacggc ataaaattca
    12421 gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt tgcgaaacca
    12481 cccgataacg gagcggcggc gttctcacga taccaacact ctcaccagat agcagcgcag
    12541 caatggcgct ggcagtgact gcaaactggc gaagatcgtt ttgtccaaag ggcaacgctt
    12601 tgccgagttc cagcgtacag gccagcgcgc caaaatgtct ggcgctgaaa tgagtaaacg
    12661 taccaccagg cgtctgatgg aataccagtg cctccagccc cgccgccccc agccacgtca
    12721 gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc ggatgcaagg
    12781 agccacgaat tgcggtatgg agatcaaggt gccagcgcac agattctttg ccctggtcat
    12841 aaaaatcttc caggcactgt tccagttcgc gcgcgcgaca ggtttccccg ctttcagcaa
    12901 atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa cgtttccctt
    12961 gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac gggatctcgc
    13021 cgtgagatat cgcgccaagc aacgcgtcca gcatctccac aggtgccgtc tcattaccgt
    13081 gtattcccgc tgaaatcacc agtgcgcctt gcggtggcgt taatggcgtc agttccagca
    13141 caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg ataaccggtt
    13201 ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc gcgctggaac
    13261 ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc ccgcccttcg
    13321 cgcagcaatt gcgggtcggc cagatcggca gcagtaagac gatcgcggta gtaacgatcc
    13381 acccagtcat tgagcgcatt aaacagcgta tcgttcatca tcaccgccgg attcaccgcc
    13441 cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc gccaccattc
    13501 gccatgcttt cacgtaaatc aaagactttt agttcgctaa tcgggttgtc agcggcaagg
    13561 agttcattga gataacccca tactccggcg tgttcccgac actcctgagg cagcaccaac
    13621 atcatggaac catcatcgcg gctcaacagt tggctgttaa acagatatgt agacaccgca
    13681 tccgacacgg aaacctgagt cgccggaact tctatcgcca taaaaccgtt cacccgcgca
    13741 cgcaggtttg ccagtaactg cacctgtcga gcgaacgcct gttgatgaca aaacagcacc
    13801 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc cctggtcgat aacgtccggg
    13861 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat tcaaccttgc caccgcctcg
    13921 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccctcttct
    13981 cgcccgtaga caaaaagttg catacccggt tcaccgtaat gaccgccgag acgattgtgg
    14041 tttgccgccc cctcatcacc gagcaaggct acctgcggca acgccgaatg gacactaaat
    14101 ttctcttcgt cgttaaaaat cgcttttaac agcgattcag agacgggcgc ttccagcgaa
    14161 cggtgaaatt tattgttcag gttagcaacg gtgagatgca ctttgccatc cagcgtatcg
    14221 gtggatggcg ctaccgtggc cgcattagct acccacattg gcgaagcgga actgacgctg
    14281 gaaagccagt gcggtgcctg gcgggcaacc ttttccagta cctgctcatc gctgccgctg
    14341 aaacccaact gacgcagcac cggaataaac ggacgctcgt gcggcgggat caccgcctgg
    14401 gggaatcccg cgtcggcaag ggctttcatt ttcagtaagc cctgcttcgc cgccagtcgc
    14461 gggttagaga cctgaaaacg gtgactggtg gaggcttcat taccaaacga caggcccgcg
    14521 taatgatgcg tcagccctac cagcccgtcg aaattgattt cccaggcgtt catcgcacca
    14581 cctcatcgga aaaatccagc ccagggttaa gcgtggcggg caatgttaac gagtccgact
    14641 ccagactcgc catcggccag gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
    14701 taccggaagc gccaatgccg ccgaatggcg cggtactggc agcgccggta agcggtttgt
    14761 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgcgcgg
    14821 gggaaaccag tccgcaagag agtccgaagc gagtgttatt cgccattcga atcgcttcat
    14881 cgaaagtatc ataacgccag acgcgcaata acggtccgaa cacctcttca tctggtacgc
    14941 cagcaacgcc tgtcatttca atgatccccg gcgtcagcaa cgatgtccct gcttgtaata
    15001 agcgcggcgc aagcagggtt cgtctgccca tcgcttccag ttgctgccag gcagtaacca
    15061 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
    15121 atttgcccgg cgttaatcgc tggctgacgg caaccagacg agcaagaaac gcatcgccct
    15181 gcgccccgct tttcagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
    15241 atgccgactg aatggtcaga tggacagcgg catcgatatc cgccacctca tcgataatca
    15301 gcgggttatt gccgcccatt tcgagggcga gaattttctc cggctgaccg gaaagctgac
    15361 gatgcaactg gtagccagta ttggcgctac cggtaaacag caagccgtcg agatcctcca
    15421 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc acgcccggcg
    15481 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc gctccagggt gtcagttcgc
    15541 tgggtttaaa gataatggtg ttacccgcca gcaatgccgg aacgatatgc ccgttcggca
    15601 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
    15661 ggctcgccgc gccgtccggc atttcgctac gctgctcgcc ggtacgaaca tgatacgcct
    15721 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
    15781 gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt tccagcaatc
    15841 cggcaaagcg ttcaactctt acctgacgat cgccaaagga gagacgcgcc cagcgcggaa
    15901 acgccgcacg ggctgcccga caagcctgcc cgacctgagc ggcatcggca tcattgcctt
    15961 gccataacac ctcgcccgat accggattac gcttcacacg caatgcgccc tggcccgtta
    16021 cccaatcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
    16081 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
    16141 acgctcggtt gccggatcgg tacgcaccag caccacgcgg aaatggtgat aattttcatt
    16201 ggcgaccaag caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
    16261 ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgtcg gcccaccgtc
    16321 gaagatgtcg atataattac ggtagcgaaa accttctttc tccagtaccg cacgggcagg
    16381 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgagata aaaagtgggt
    16441 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
    16501 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgctta ccgaggcttt gccagaacgg
    16561 tgaatagcca tgttcgtcaa tcaccccgcg catttcggca accactttgt cattaaactt
    16621 gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc cgttgccctc
    16681 tttgcgccac tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
    16741 actgagaaac agcgtcggca acgcgttata gacattcagc tctttcgaag cgtgaaccag
    16801 cgtgccgacg cgatagttgt accagggatc gttcagccca actgccacct caatggcgca
    16861 aatccccgcc actgtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcact
    16921 tttgggcagt tcgccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
    16981 ttcattggcg ggaagcgacg tcaggccgcc gccagttttg ctggcaagct gcatcagcgc
    17041 cgagatatct gaacgctcaa cgggacggat gaccatcatg aagaacctcg gctaacaaag
    17101 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctaacattg
    17161 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
    17221 gccgcttcct gagagatctg tttcgcttgc ccggcgtaat cggcattcaa cacgcaacca
    17281 atcaacaaac ctaagccgcg aacttcactg aacaaaccgt agcggtgatt aatggtatta
    17341 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggcgtgttg
    17401 atgagatcca gcactttgcc tgccaccgcc gaggccagcg gattgccgcc ataggtggtg
    17461 ccatgagtgc caacggtcat cacactggcg cactcttcgg ttgccaacaa cgcaccaacc
    17521 gggaaaccgc cgcccagcgc tttggcggta gttaacagat cgggcgtcac gccgtagtgc
    17581 atataggcat acagttcccc ggtgcgtccg acgccggttt gtacttcatc aaaaatcagc
    17641 aatgcattgt ggcggtcaca caattcacgc agaccatgta aaaacgcgtt gctggctggc
    17701 accacaccgc cttccccctg gatgggttcg acaatcaccg cacaggtaga gtcgtcaatc
    17761 agcgcgctgg cagagttaat atcgttatat gcggcatgaa gaatatccgg cggcagtggc
    17821 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    17881 tgaaacgcat ttttaaacgc cacgataccg ctcttatggc tgccgtagcg gtcgtgagcg
    17941 aatttacgcg ccagttttag cgccgcttca ttggcttccg caccggagtt acaaaagaag
    18001 acgcgatcgg caaacgtggc gtcggtcaat tttttcgcca gtcgcagtac cggctcgttg
    18061 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ctcacgcagt
    18121 tccgggtgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    18181 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa cggtgccgga
    18241 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    18301 acctctacag taaataattc gttatttata tgttaacaat aagtaatgtt tgcgctgtaa
    18361 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaatcgc
//
LOCUS       DARNVM010000095        17955 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.095, whole genome shotgun sequence.
ACCESSION   DARNVM010000095 DARNVM010000000
VERSION     DARNVM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.095"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1042
                     /locus_tag="SI621_002929"
     CDS             <1..1042
                     /locus_tag="SI621_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065619.1"
                     /translation="LMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCG
                     VKVINDWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFL
                     AIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFS
                     GINYTAWLYLAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGM
                     APIKSRKICIIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVA
                     VASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIF
                     LVRQPINDPRKD"
     gene            complement(1091..2755)
                     /gene="tsr"
                     /locus_tag="SI621_002930"
     CDS             complement(1091..2755)
                     /gene="tsr"
                     /locus_tag="SI621_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEI4065620.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(2874..3320)
                     /gene="hpaR"
                     /locus_tag="SI621_002931"
     CDS             complement(2874..3320)
                     /gene="hpaR"
                     /locus_tag="SI621_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEI4065621.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            3592..4881
                     /locus_tag="SI621_002932"
     CDS             3592..4881
                     /locus_tag="SI621_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HEI4065622.1"
                     /translation="MKGTIFAVALNHRSQLDVWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEGDAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4878..6344
                     /gene="hpaE"
                     /locus_tag="SI621_002933"
     CDS             4878..6344
                     /gene="hpaE"
                     /locus_tag="SI621_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HEI4065623.1"
                     /translation="MKKINHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6346..7197
                     /gene="hpaD"
                     /locus_tag="SI621_002934"
     CDS             6346..7197
                     /gene="hpaD"
                     /locus_tag="SI621_002934"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HEI4065624.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7207..7587
                     /locus_tag="SI621_002935"
     CDS             7207..7587
                     /locus_tag="SI621_002935"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HEI4065625.1"
                     /translation="MPHFIVECTENIRQEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            7652..8455
                     /gene="hpaH"
                     /locus_tag="SI621_002936"
     CDS             7652..8455
                     /gene="hpaH"
                     /locus_tag="SI621_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEI4065626.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8466..9254
                     /gene="hpaI"
                     /locus_tag="SI621_002937"
     CDS             8466..9254
                     /gene="hpaI"
                     /locus_tag="SI621_002937"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEI4065627.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETSEAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            9372..10748
                     /gene="hpaX"
                     /locus_tag="SI621_002938"
     CDS             9372..10748
                     /gene="hpaX"
                     /locus_tag="SI621_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HEI4065628.1"
                     /translation="MSDTSPAIPESIDPANQHKTLTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10758..11648
                     /gene="hpaA"
                     /locus_tag="SI621_002939"
     CDS             10758..11648
                     /gene="hpaA"
                     /locus_tag="SI621_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HEI4065629.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            11899..13461
                     /gene="hpaB"
                     /locus_tag="SI621_002940"
     CDS             11899..13461
                     /gene="hpaB"
                     /locus_tag="SI621_002940"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HEI4065630.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13479..13991
                     /locus_tag="SI621_002941"
     CDS             13479..13991
                     /locus_tag="SI621_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HEI4065631.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14384..16534
                     /gene="btsT"
                     /locus_tag="SI621_002942"
     CDS             14384..16534
                     /gene="btsT"
                     /locus_tag="SI621_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HEI4065632.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16584..16787
                     /locus_tag="SI621_002943"
     CDS             16584..16787
                     /locus_tag="SI621_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEI4065633.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16798..17754
                     /gene="yjiA"
                     /locus_tag="SI621_002944"
     CDS             16798..17754
                     /gene="yjiA"
                     /locus_tag="SI621_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEI4065634.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(17823..>17955)
                     /locus_tag="SI621_002945"
     CDS             complement(17823..>17955)
                     /locus_tag="SI621_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEI4065635.1"
                     /translation="EKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         4120 a         4903 c         4801 g         4131 t
ORIGIN      
        1 cctgatgctg ggactgggga tgttcttctg gtcactgttc caggccatgt ctggcatggt
       61 gcacagcttt acgcagttcg tgttggtgcg tatcggtatg gggattggtg aagcgccgat
      121 gaacccatgt ggcgtaaaag tcattaacga ctggttcaac atcaaagagc gcggacgccc
      181 gatgggcttc ttcaacgcag cttctaccat tggcgttgcc gtaagcccac cgattctggc
      241 ggcgatgatg ctggtgatgg gctggcgtgg gatgtttatt accattggtg tgctggggat
      301 ttttctcgcc attggctggt atatgctcta tcgcaaccgc gagcacgtag aactgactgc
      361 cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc cgccgagatc cgctcagttt
      421 tgccgaatgg cgcagcctgt tccgtaaccg cacaatgtgg ggaatgatgc tcggattcag
      481 tggcatcaac tacactgcgt ggctgtatct ggcctggctt cctggttacc tgcaaacagc
      541 ctataacctg gatttaaaaa gcacagggtt gatggcggct atccctttcc tgtttggggc
      601 tgccgggatg ctggtcaacg gttacgttac tgactggctg gtcaaagggg gaatggctcc
      661 gattaaaagc cgtaagatct gcattattgc cgggatgttc tgttctgccg cctttacgct
      721 ggtcgtaccg caagcgacaa catccatgac ggcggttctg ctgattggca tggcactgtt
      781 ctgtattcac tttgccggaa catcctgctg gggcttgatc cacgtcgcag ttgcttctcg
      841 catgacggcg tcggtgggca gtatccagaa ctttgccagc ttcatctgcg cctcttttgc
      901 gccgatcatt actggtttta ttgttgatac cacccactca ttccgtctgg cactaatcat
      961 ctgcggttgc gtcaccgcag cgggagcact ggcgtacatc ttcctggttc gtcagccgat
     1021 caacgacccg cgtaaagatt aaactcccta tgcccgataa cattttgctt atcgggcatt
     1081 tttatggcga ttaaaacgtt tcccagttct ccccgctatc tgccacggcc attttacgcg
     1141 gcgtagctgg cgtcacggtt tttactacag ccgatgtttc acgctgctgt tgctgctgtt
     1201 gctgaatccg gaacactgcc acagcttcgg tcaggcgact ggcctgctct tccagcgcgg
     1261 cggcggcagc ggcagattct tccaccagcg cagcgttctg ttgagttacc cggtccatct
     1321 cagcaaccgc taagccaacc tgatcgatac cacggctctg ctcatcagaa gcagaagcaa
     1381 tttcgcccat aatgtccgtc acgcgggtca cggcgctgac aatctccgcc attgtttccc
     1441 cggcgctttc gaccagcgta gagccaacat ccactttccc caccgagtct tcaatcaggc
     1501 ttttaatttc acgagccgcc tgggcgctgc gctgggccag attacgcact tctcccgcga
     1561 ccaccgcaaa accgcgccct tgctcacccg cacgcgcagc ctcaaccgcg gcgttcaaag
     1621 ccagaatatt ggtctggaag gcaatgccgt caattacgct gataatatcg gcgattttct
     1681 gcgaactggt ggagatatcg cgcatggtct gtactacgtt atccaccact ttgccgccgc
     1741 gttgcgccgt ttcagaagca cttaacgcca gatggctggc ctgacgcgca ttctcggcgt
     1801 tctgtttcac cgttgcggtc agttgctcca tgctggctgc cgtctcttcc agcgaagcgg
     1861 cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt ggcgatttcg ctggcaccgc
     1921 tataaatggc attggccccg ttgcgcacat caccgacggt acgcatcagc tctccctgca
     1981 tatggcgcaa actctctgcc agttgcccca tctcattaga gccatccacc tcaatcggtt
     2041 tcaccagatc gccgcctgca atatgacgaa tgctgtcaat caggcgattc attggcgcta
     2101 ccaacgaggc tttaataccg aaccagacgg cgaagatgac cgccagtacg acgatcatca
     2161 cgcccaccag aatccacatc gcctggctgt aggaggcatt attatcgctg acggcgatat
     2221 catagagccg atcgttttgc tccatgtaag ccacatactg cttctcgaaa ccgtcctgat
     2281 atccctgggt cggctgatca aagaactcgt tgattttgcc tgcacctaac agttggatca
     2341 gctccgccag cgcattgtga taaatatcgt aattacgttt gatctctgcc gctgcggcgg
     2401 tgctctgacg cgggtcacgc ggcaacgctt cgtaatccgc ccagtttttt tccgcctgtt
     2461 tcagcgaaat actggcactc tgcatcagct cagcaacggt tgaaccgcta ccaatattat
     2521 tctgatccat catgtagcgg atacccgcgc ggttgagggt gttacgcgtc tgcaacaacg
     2581 cgacccagct gccattcagc gtggattgct gctggcgaat ggtttgtaaa acagtgaaat
     2641 tttctttgtc attctttaag gcattaaaga acagaccgcc tgatgtcagt tgtaaaaggc
     2701 caaaaacggc caaaaccagc agtaagctgg tcacaatttt gatacgtttt aacatggttt
     2761 ctctttcctg tggaccgatg caagattttc ggcctggaaa gcaaaaactt tatgaaaagg
     2821 tgtaacccgc catccatgga aaaaccaggc gctggttatc agatattaaa aagttattca
     2881 ttatcgcccg ggatgtcttc ttgtcgggaa ttacccagag caataaattc ctctaacaga
     2941 tgggttaact gttgcatttt ctcggcagta aactgagctt caatctgccg ataggcttct
     3001 tcaatctgtg tctgagcgcg gttatacagg gcctgcccct ctttggtcag cgagatatag
     3061 agcttccgct gatcattgat cggctttaat cgcaacacta aaccgtcgcg ttccatccgc
     3121 gtgaggatcc cggtcaggct tgggcgcaaa atgcaggcgc gatacgccag atcgtgaaaa
     3181 tccattgatg ggctttccgc cagaatacgc acgatgcgcc actgttgctc agtcaaatta
     3241 tgccgtttaa cgattgggcg aaaataactc atcgccgctt ctcgcgcctg gagcaacgca
     3301 atggttagtg agtcgtgcat tatctttccc ctgaaaaatt caaaatcatt aatatagaaa
     3361 cagttaatca tcacgttacc gggactgagc tatccccgca attcctcatt ttatcaaaat
     3421 tgagcatact ttattaactt actgatattt agtattttat tacctgcaaa tgtaaatagt
     3481 ttgttaatta gatcacattt acatcactta cttttgccaa actacgcaat cgggaataaa
     3541 agtaatcatt aacatattaa tgattaaaat gatcgaatcc gaggagtggt aatgaaaggc
     3601 actatcttcg ccgtagcgtt gaaccatcgc agccagcttg atgtatggca ggaagcgttc
     3661 cagcaatccc cctacaaagc cccgcctaaa actgcggtct ggtttattaa accgcgcaat
     3721 acggtgattg gttgcggtga accgattccc tttccacagg gtgaaaaggt actgagcggt
     3781 gcgacagttg cgctgattgt gggaaaaacg gcgacgaaag tacgtgaaga agatgcagca
     3841 gagtacatcg ccggatatgc gctggctaac gacgtcagcc tgccggaaga gagcttttac
     3901 cgcccggcaa tcaaagcaaa atgtcgtgat ggattctgcc ccattggcga aaccgtggct
     3961 ctcagcaatg tcgataatct gaccatctat accgagatca acggacgtcc tgccgatcac
     4021 tggaacaccg ccgatttaca acgtaacgcc gcgcagttgc tgagcgctct gagcgaattt
     4081 gccacgctga atccaggcga tgccattctg ctcggcacgc cacaggcgcg cgtggaaata
     4141 cagccaggcg atcgcgttcg tgttctcgct gaaggtttcc cgccactgga aaatccggta
     4201 gtggacgaac gtgaagtgac cacgcgcaag agcttcccaa cgcagccaca cccgcacggc
     4261 acgctgtttg ccctcggcct gaactacgcc gaccacgcca gcgaactgga atttaagcca
     4321 ccggaagaac cgctggtgtt cctgaaagcg ccgaataccc tcactggcga taaccagacc
     4381 tccgtgcgcc cgaacaatat tgaatacatg cactacgaag cggagctggt ggtagttatt
     4441 ggcaagcagg cgcgtaacgt cagcgaaggc gatgccatgg attatgtcgc gggctacacc
     4501 gtgtgtaacg actacgccat tcgcgactat ctggaaaact actaccgccc taacctgcgg
     4561 gtaaaaagcc gcgacggact gacgccgatg ctttcaacca tcgtgccgaa agaggcgatc
     4621 ccggacccgc ataatctgac ccttcgcacc ttcgtcaacg gcgagttacg ccagcaaggc
     4681 accaccgccg atctgatctt cagcgtgccc ttcctgatcg cctacttaag cgaatttatg
     4741 accctgaatc cgggcgacat gatcgccacc ggcacaccaa aaggcttatc tgacgtggtg
     4801 cctggcgatg aagtagtggt ggaagtagaa ggcgtgggcc gcctggtgaa ccgaattgtg
     4861 agtgaggata cagcgaaatg aaaaaaataa accattggat caacggcaaa aatgttgcag
     4921 gtaacgacta cttccagacc accaatccgg caacgggtga agtgctggcg gatgtggcct
     4981 ctggcggtga agcggagatc aatcaggcgg tagcggcagc gaaagaggcg ttcccgaaat
     5041 gggccaatct gccgatgaaa gagcgtgcgc gcctgatgcg ccgtctgggc gatctgatcg
     5101 accagaacgt gcctgagatc gccgcgatgg aaaccgctga cacgggcctg ccaatccatc
     5161 agaccaaaaa tgtgttgatc ccacgcgctt ctcacaactt tgaatttttc gcggaagtct
     5221 gccagcagat gaacggcaag acctatccgg ttgacgacaa gatgctcaac tacacgctgg
     5281 tgcaaccggt aggcgtttgt gcgctggtat cgccgtggaa cgtgccgttt atgaccgcca
     5341 cctggaaggt cgcgccgtgt ctggcgctgg gcaataccgc ggtgctgaag atgtcggaac
     5401 tctccccgct gaccgctgac cgcctgggtg agctggcgct ggaagccggt attccggcag
     5461 gcgtgctgaa cgtggtacag ggctacggcg caaccgcagg ggatgcgctg gttcgtcatc
     5521 atgacgtacg tgccgtgtcg ttcaccggcg gtacggccac cgggcgcaac atcatgaaaa
     5581 acgccgggct gaaaaaatac tccatggaac tgggcggtaa atcgccggtg ctgatttttg
     5641 aagatgccga tattgaacgc gcgctggacg ccgccctgtt caccatcttc tcgatcaacg
     5701 gcgagcgctg caccgccggt tcgcgcatct ttattcagca aagcatctac ccggaattcg
     5761 ttaaacgctt tgccgaacgc gccaaccgtc tgcgcgtggg cgatccgaac gatccgaata
     5821 cccaggttgg ggcgcttatc agccagcaac actgggaaaa agtctccggc tatattcgtc
     5881 tcggcattga agaaggcgca accctgctgg cgggcggtcc ggataaaccg tccgacctgc
     5941 ctgcgcacct gaaaggcggc aacttcctgc gcccaacggt gctggctgac gttgataacc
     6001 gtatgcgcgt cgcccaggaa gagattttcg ggccggtcgc ctgtctgctg ccgtttaaag
     6061 acgaagctga aggtctgcgc ctggcaaatg acgtggagta tggcctcgcg tcgtacatct
     6121 ggacgcagga tgtcagcaaa gtgttacgcc tggcgcgtgg cattgaagca ggcatggtgt
     6181 tcgtcaacac ccagaacgtg cgcgacctgc gccagccgtt tggcggcgta aaagcctccg
     6241 gcaccgggcg tgaaggcggt gagtacagct tcgaagtgtt cgcggaaatg aagaacgtct
     6301 gcatttccat gggcgaccat ccaattccga aatggggagt ctgatatggg taagttagcg
     6361 ttagccgcca aaatcacgca cgtaccgtcg atgtatctct ctgaactgcc agggaaaaac
     6421 cacggttgcc gccagggcgc gatcgacggg cataaagaga tcagcaagcg ttgccgggaa
     6481 atgggcgtcg ataccattat cgttttcgat acccactggc tggtcaacag tgcttatcac
     6541 atcaactgtg cagaccattt tgaaggcgtc tacaccagca acgagctgcc gcattttatt
     6601 cgtgacatga cctacaacta cgagggcaac ccggagttgg ggcagcttat tgccgatgaa
     6661 gccttaaaac tcggcgtgcg ggcaaaagcg cacaatattc ccagcctgaa actggaatat
     6721 ggcacgctgg tgccgatgcg ctacatgaat gaagacaagc acttcaaagt ggtctccatc
     6781 tccgcattct gtaccgttca tgattttgcc gacagccgca agctgggcga agcgattctg
     6841 aaagcgatcg aacagtacga cggcaccgtg gcggtccttg ccagcggttc gttatcgcac
     6901 cgctttattg acgatcagcg tgcggaagaa gggatgaaca gctacacccg cgagttcgac
     6961 cgccagatgg acgagcgcgt ggtgaagttg tggcgcgaag gccagttcaa agagttctgc
     7021 aatatgctgc cggagtacgc tgactactgc tacggcgaag gcaatatgca cgacacggtg
     7081 atgctgctgg ggatgctcgg ctgggataaa tacgacggca aggtagagtt tatcaccgag
     7141 ctgttcccaa gctctggcac cggtcaggtt aacgctgttt tcccgctgcc cgcgtaagga
     7201 accaccatgc cgcactttat cgtggaatgt actgaaaata ttcgtcaaga agcccgctta
     7261 cctgagctgt ttgccagtgt taacactgca ctggcggcga cggggatttt tccgcttggg
     7321 ggaatacgta gccgtgcaca ctggatcgat acctggcaaa tggctgatgg caaacacgat
     7381 tacgcctttg tgcatatgac gctaaaaatc ggcagcgggc gcagtctgga aagccgtcag
     7441 gaagtgggcg aaatgctgtt tgatctgatt aaaacgcact tcgcctcact gatggagagc
     7501 cgttacctgg cgctttcatt tgagattgca gagctgcacc cgacgttgaa tttcaaacag
     7561 aacaacgtac acgcgttgtt taaataaatt tgcaccagct tccaggccgg ataaggcgca
     7621 accgcatccg gcaatgccac aggatatcgc tatgttcgac aaacacaccc acaccctgat
     7681 cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga tctccctgga
     7741 ttacccggag atcaccatcg aagacgctta cgcggtgcag cgtgaatggg ttcgcctgaa
     7801 aatcgccgaa ggccgtacgc tgaaaggcca caaaatcggc ctgacctcga aagcgatgca
     7861 ggccagctca caaatcagcg aaccagatta cggtgccctg ctggacgaca tgttcttcca
     7921 cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag tggagctggc
     7981 ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg tttacaacgc
     8041 cacggactac gtgatcccgg cactggagct gatcgacgct cgttgccaca acatcgatcc
     8101 ggaaacccag cgcccgcgca aagtgttcga caccatttct gataacgccg ccaatgccgg
     8161 ggtgatcctc ggtggtcgtc ccattaagcc cgatgagttg gatctgcgtt ggatctccgc
     8221 cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg tgctgaatca
     8281 cccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg tacaactgga
     8341 agccgggcaa atcattctcg gcggttcgtt cacccgcccg gttccggcgc gtaagggcga
     8401 caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg tttaaggaga
     8461 gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgcccgcag atcggattat
     8521 ggctggggct gagcagcagc tacagcgcag agttactggc cggagcagga ttcgactggt
     8581 tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc cagctacagg
     8641 cgattgcgcc ctaccccagc cagccggtag tacgtccgtc gtggaacgat ccggtgcaaa
     8701 tcaaacaact gctggacgtc ggcacacaaa ccttgctggt gccgatggta caaaacgctg
     8761 acgaagcccg tgaagcggta cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg
     8821 gcagtgcgct ggcccgcgcc tcgcgctgga atcgtattcc tgattacctg caaaaagcca
     8881 acgatcaaat gtgcgtgctg gtgcagatcg aaactagtga ggcaatgaag aacctaccgc
     8941 agattctgga cgtggaaggg gtagacggcg tgtttatcgg cccggcggat ctgagcgccg
     9001 atatgggtta tgcaggtaat ccgcagcacc cggaagtgca ggccgccatt gaacaggcga
     9061 tcgtgcagat ccgtgaagcg ggcaaagctc cggggatcct gatcgccaat gagcaactgg
     9121 caaaacgcta cctggaactg ggcgcgctat ttgtcgccgt cggcgttgac accaccctgc
     9181 tcgcccgcgc cgccgaagcg ctggcagcac ggtttggcgc gcaggccacg accgttaagc
     9241 ccggcgtgta ttaatgctga atgcgcttat ccggcttaca gcagtaccaa actgtagccg
     9301 gataaggcgt ttacgccgca tccgccataa atcttgtcgt accctacaaa aatcccatta
     9361 gaggaagaaa aatgagcgac acctcacctg ccataccgga aagtatcgat ccggcaaatc
     9421 agcataaaac gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga
     9481 tcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg
     9541 ccgggctaac gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgccacca
     9601 ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg
     9661 tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctacggcca
     9721 ccatgtttgc cactggcccc accagcttat acgtactgcg tatactggtt ggcattaccg
     9781 aagccggctt tctgcctggc attctgctgt atttaacctt ctggtttccg gcctatttcc
     9841 gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg ctgggatcga
     9901 tcgtttccgg ctacattttg tcgctggatg gcgtaatggc attaaaaggc tggcagtggc
     9961 tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg
    10021 atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga
    10081 tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagt caccacgcca
    10141 tgcaacaacg cagcatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg
    10201 cgtatttctg cctgaccaac acacttagtg caatcagcat ctggacaccg cagatcctac
    10261 aaagctttaa tcagggcagc agtaatatca ccatcggcct gctggccgcc gtaccgcaga
    10321 tttgtaccat tctcgggatg atctactgga gccgtcactc agatcgccgc caggaacgaa
    10381 ggcatcacac cgcccttcct tatttgttcg ctgccgctgg ttggttactg gcttcggcaa
    10441 ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg
    10501 caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg
    10561 gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg
    10621 gctggttgaa agatctgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc
    10681 tggtgattgg tgcggggatt atctgggcaa ttccaatgca gtcctcccgt ccgcgagcga
    10741 ccccgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt
    10801 acgatgaaag cctgggcacg gacgatgtgc attatcagtc cttcgcccgc atggcggcat
    10861 tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca
    10921 atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt
    10981 ttgtcctgac gccgccgtca gtacctcatg cgtttattac ggagtctgac gccgacggtc
    11041 atgtgttgac ggtacgggaa gatctgatct ggcccctgct ggaagttctt tatccgggca
    11101 ctcgggaaac cttcggcctg ccggggattt gcctgtcact ggcagataaa cccgacgaac
    11161 tggcagcgct ggaacactat tggcaactga tagagcggga atcggtggaa caactgcctg
    11221 gacgggaaca caccctgacg ttactggcac aggcagtatt caccctactg ctgcgtaacg
    11281 caaaactcga cgaccatgcc gccagcggga tgcgcggaga attaaaactg ttccagcgtt
    11341 ttaatatgct gattgaaagc cattttcatc agcactggac agtaccggat tacgctaacg
    11401 aactgcatat caccgaatca cgtctcacgg acatctgccg ccgctttgcc aaccgtccgc
    11461 caaaacggtt gattttcgac aggcagctgc gagaagccaa gcggctgctg ctgttttctg
    11521 ataacgccgt gaacaatatt gcctggcaac tcggttttaa ggatccggct tattttgcgc
    11581 gcttttttaa tcgtttagtc ggttgctcgc ccagtgctta tcgtgccaaa aaagtacctg
    11641 tgacgtgaaa atcccttagc cttaacggga aaccaggcac cacctgctat tcccctttct
    11701 ccgcctggag caaggggatt tagtaacgtc aaaaatcgct aaaagcgatc tcgatcacaa
    11761 aaccgtaccc gacctgaaaa gtaccagcca ttgtcccaaa agtgtcttcc cgtaatcgcc
    11821 cctgcaagct tcaattgaat aacaaaaaca acacacaaaa ttaacaatcg tattccgatt
    11881 aatactgtag aggtcgacat gaaaccagaa gatttccgcg ccagtaccca acgtccgttc
    11941 accggggaag agtatctgaa aagcctgcag gatggtcgcg agatctatat ctacggcgag
    12001 cgagtgaaag acgtcaccac tcatccggca tttcgtaatg cggcagcgtc tgttgcccaa
    12061 ctgtacgacg cgctgcacaa accggagatg caggactctc tgtgttggaa caccgacacc
    12121 ggcagcggcg gctataccca taaattcttc cgcgtggcga aaagtgccga cgacctgcgc
    12181 cagcaacgcg acgccatcgc tgaatggtca cgcctgagct atggctggat gggccgtacc
    12241 ccagactaca aagccgcttt cggttgcgca ctgggcgcga atccgggctt ttacggtcag
    12301 ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg aaactggcct ctactttaac
    12361 cacgcgattg ttaacccacc gattgatcgt catttgccga ccgataaagt gaaagacgtt
    12421 tacatcaagc tggaaaaaga gactgacgcc gggattatcg tcagcggtgc gaaagtggtt
    12481 gccaccaact cggcgctgac tcactacaac atgattggct tcggctcggc acaagtgatg
    12541 ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa tggatgccga tggcgtgaaa
    12601 ttaatctccc gcgcctctta tgaaatggtg gcgggtgcta ccggctcgcc gtacgactac
    12661 ccgctctcca gccgctttga tgagaacgat gcgattctgg tgatggataa cgtgctgatc
    12721 ccatgggaaa acgtactgat ctaccgcgat ttcgatcgct gccgtcgctg gacaatggaa
    12781 ggtggttttg cccgtatgta tccgctgcaa gcctgtgtgc gcctggcagt gaaattagac
    12841 ttcattacgg cactgctgaa aaaatcactc gaatgtaccg gcaccctgga gttccgtggt
    12901 gtgcaggccg atctcggtga agtggtggcg tggcgcaaca ccttctgggc attgagtgac
    12961 tcgatgtgtt ctgaagcgac gccgtgggtc aacggggctt atttaccgga tcatgccgca
    13021 ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga agatcaaaaa cattatcgaa
    13081 cgcaacgtta ccagtggcct gatctacctc ccttccagtg cccgtgacct gaacaatccg
    13141 cagatcgacc agtatctggc gaagtatgtg cgcggttcga atggtatgga tcacgtccag
    13201 cgcatcaaga tcctcaaact gatgtgggat gccattggca gcgagtttgg tggtcgtcac
    13261 gaactgtatg aaatcaacta ctccggtagc caggatgaga ttcgcctgca atgtctgcgc
    13321 caggcacaaa gctccggcaa tatggacaag atgatggcga tggttgatcg ctgcctgtcg
    13381 gaatacgacc agaacggctg gactgtgccg cacctgcaca acaacgacga tatcaacatg
    13441 ctggataagc tgctgaaata acgcagcagg aggttaagat gcaattagat gaacaacgcc
    13501 tgcgctttcg tgacgcgatg gccagcctgt cggcagcggt aaatattatc accaccgagg
    13561 gcgacgccgg acaatgcggg attacggcaa cggctgtctg ctcggtcacg gatacaccac
    13621 cgtcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa cccggttttt cagggcaacg
    13681 gcaagttgtg cgtcaacgtc ctcaaccatg agcaggaact gatggcacgc cacttcgcgg
    13741 gcatgacagg catggcgatg gaagagcgtt ttagcctctc atgctggcaa aaaggtccgc
    13801 tggcgcagcc ggtgctaaaa ggttcgctgg ccagtcttga aggtgagatc cgcgatgtgc
    13861 aggcaattgg cacacatctg gtgtatctgg tggagattaa aaacatcatc ctcagtgcag
    13921 aaggtcacgg acttatctac tttaaacgcc gtttccatcc ggtgatgctg gaaatggaag
    13981 ctgcgattta agctccagcc ccttcacgca ttcgtcgaag gggcattttt attgtgatct
    14041 caatcacata tccacccgat gaataattct tgcccaaccg ctcaccgcca ttcctgaccg
    14101 cttacatccc taaaataacc actcagttat ttaccttact ttacgcgcgc gtaactctgg
    14161 caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg
    14221 gcttcggcct atgattaaca aattactcta tggattttgg tttgcaggaa ggcagcaagt
    14281 gagtgaatcc ccgggagctt acaacagtaa gtgacagggg tgaacgaacg cagctgccgc
    14341 acctgtaagc caaaagacga cgagtaaaat gccaggtttt actatggata cgaaaaaact
    14401 attcaagcac ataccctggg tgattctcgg aatcatcggt gcattctgcc tcgcggtagt
    14461 tgcattacgt cggggggagc acgtcagcgc cctgtggatc gtggtcgcct ctgtgtcggt
    14521 gtatctggtg gcttatcgct actacagtct gtacatcgcc cagaaggtga tgaaactcga
    14581 ccccacgcgc gcgacgcctg cggttattaa caacgacggt ctgaactacg ttccgaccaa
    14641 ccgttacgtg ttgttcggtc accacttcgc cgctatcgcc ggtgctggtc cgctggtggg
    14701 tccggttctc gccgcgcaga tgggctacct gcctggcacg ctgtggctgc tggcgggggt
    14761 agtgctggcc ggtgcggtac aggactttat ggtgctgttt atttcctctc gccgtaacgg
    14821 cgcatctctc ggtgagatga tcaaagaaga gatgggacca gtaccgggga ctatcgcgct
    14881 gtttggctgt ttcttaatca tgatcatcat cctcgccgtt ctggcgctga ttgtggttaa
    14941 agccctggcc gaaagtccgt ggggtgtctt caccgtttgc tcaaccgtac cgattgcgct
    15001 gtttatgggt atctacatgc gctttatccg tccggggcgt gtgggtgaag tctctgtcat
    15061 tggtatcgtg ctgctggttg cctctatcta cttcggtggc gtgattgctc acgatccgta
    15121 ctggggtccg gcactgacct ttaaagacac caccattacc ttcgcgctga ttggctatgc
    15181 gtttgtttcc gcactactgc cggtgtggct gatcctcgca ccgcgcgact atctggcaac
    15241 cttcctgaaa atcggtgtta tcgtcggcct ggcgctgggt atcgtggtgc tgaacccgga
    15301 actgaaaatg cctgcgatga cccagtacat tgacggtact ggcccgctgt ggaaaggcgc
    15361 tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct tccacgcact
    15421 gatctcttcc ggtacgacgc cgaaactgct ggctaacgaa accgacgcgc gtttcattgg
    15481 ctacggcgca atgctgatgg agtccttcgt ggcgattatg gcgctggttg ctgcgtccat
    15541 catcgaaccg ggtctttact tcgcgatgaa taccccgcct gctggccttg gcatcaccat
    15601 gcctaacctg catgaaatgg gtggcgaaaa cgcgccgatc atcatggcgc agctgaaaga
    15661 cgttaccgca catgcggcag cgaccgtcag ctcctggggc ttcgtgattt cgccagagca
    15721 gatcctgcaa accgcgaaag acattggtga gccttctgtc ctgaaccgtg caggtggtgc
    15781 gccgacgctg gcggtaggta tcgctcatgt gttccacaaa gtgctgccga tggctgacat
    15841 gggcttctgg tatcacttcg gtattctgtt cgaagcactg ttcatcctga ccgcgctgga
    15901 tgcgggtacc cgttctggcc gctttatgct gcaagacctg ctgggtaact tcatcccgtt
    15961 cctgaaaaaa accgactctc tggttgccgg tatcatcggt actgcgggct gtgtgggtct
    16021 gtggggctac ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta agagcctgtg
    16081 gccgctgttc ggtatctcca accagatgct ggcagccgta gcgctggtac tgggcaccgt
    16141 tgtgctaatt aagatgaagc gcacccaata catctgggta actgttgttc cggctgtatg
    16201 gctgcttatc tgcaccacct gggcgctggg cctgaaactg ttcagcacca acccgcagat
    16261 ggaaggcttc ttctacatgg caagccagta caaagagaag attgctaacg gtactgacct
    16321 gacggcgcag cagattgcca acatgaacca catcgttgtg aacaactaca ccaacgcagg
    16381 cctgagtatt ctgttcctga ttgtggtgta cagcatcatc ttctacggtt tcaaaacctg
    16441 gcttgcggtg cgtaacagcg acaaacgtac tgacaaagaa acaccgtacg ttccaatccc
    16501 ggaaggcggc gtgaagatct cttcgcacca ctaaccgtgt ttagccccgc ttcggcgggg
    16561 ctttgttctt atcagagtga actatgtttg gtaacttagg acaggcaaaa aaatatctcg
    16621 gccaagcggc gaagatgttg attggcattc cagactacga caactacgtc gagcatatga
    16681 agaccaacca tcccgacaag ccgtacatga gctatgaaga attcttccgc gaacgccaga
    16741 acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg agagagcatg
    16801 aacccgattg cagttaccct actcaccggt tttttaggcg cgggcaaaac caccctgctg
    16861 cgccatattc ttaacgaaca acacggctac aagattgccg tgattgaaaa cgaattcggc
    16921 gaagtctccg ttgatgatca attgattggc gaccgcgcca cgcagatcaa aacgctgacc
    16981 aacggctgca tctgctgttc gcgctccaac gagctggaag acgcgctact cgacctgctg
    17041 gataatctcg acaagggcaa tattcagttc gatcgtctgg tcattgaatg caccggcatg
    17101 gccgatcccg gcccgattat tcagaccttt ttctcccatg agattttatg ccagcgctac
    17161 ctgctggacg gcgtgattgc gctggtggat gcggtacatg ccgatgagca gatgaaccag
    17221 ttcaccatcg cccagtccca ggtgggctac gctgaccgta ttctgctgac taaaaccgac
    17281 gtcgcaggcg aagcagagaa actgcgtgaa cgcctggcgc gcatcaacgc ccgcgcaccg
    17341 gtctacaccg tcacccacgg tgacatcgac ctgggcctgt tgttcaacac caacggtttt
    17401 atgctggaag aaaacgtcgt cagcaccaaa ccgcgtttcc actttatcgc ggataaacaa
    17461 aacgatattt cgtcgattgt ggtggaactg gattacccgg tagatatcag cgaagtttcc
    17521 cgcgtgatgg aaaacctgct gctggaatcg gcagataaac tgctgcgtta caaagggatg
    17581 ctgtggattg acggcgaacc taaccgcctg ctgttccagg gcgtccagcg cctctacagc
    17641 gccgactggg acaggccgtg gggcgatgaa aaaccgcata gcacgatggt gtttatcggg
    17701 attcagctgc cggaggatga gattcgggcg gcgtttgcgg ggttgaggaa gtaagttcgg
    17761 gaaagatcaa ctttttgaga gttccaccgc aacattatta caagtggaac tctttagata
    17821 tgtcagacca accaaagctt cttcaattgc gttttcattt tttcatgtct cattttcagt
    17881 tcagccaatc cccattctga agaattacaa acatcttttg ttaaatcaaa agagctttta
    17941 ctattaagtt tctca
//
LOCUS       DARNVM010000096        17868 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.096, whole genome shotgun sequence.
ACCESSION   DARNVM010000096 DARNVM010000000
VERSION     DARNVM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.096"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..579
                     /locus_tag="SI621_002946"
     CDS             <1..579
                     /locus_tag="SI621_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HEI4065636.1"
                     /translation="ATRGLITAFDIDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWD
                     TDGLGYDQGVWQGPYDPDSGYMYLSDETYRVILKAKIAINNWDGRNDSLPAIHDTATA
                     GSGLRMQIVDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAGVWAGGIETPS
                     VETPSEGSKFFGFDMDNEFISGFDVGAWGVLL"
     gene            579..1631
                     /locus_tag="SI621_002947"
     CDS             579..1631
                     /locus_tag="SI621_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065637.1"
                     /translation="MAKNDFKAFATGKNANVMSQKEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGDGTGRLIAVYRIDTSRQITLPKDARFVVADLIGGGGGGGSNSTT
                     VQPGYASAGQGGNSGSRVVAKFTVDASQKINITIGAGGAGGVPTPATGANGSDGGATI
                     IDGITATGGRGGAKGAAFNVLPTGGPTASPNANNTIPGRAELIAQHIGYGGNSGFLFS
                     DKNALGGKGADSEFGVGGVAIGISSGVNASAGNNARGNGSGGSGAVSIYGSGSTGVSM
                     SGGAGADGLAILWVYG"
     gene            1631..2038
                     /locus_tag="SI621_002948"
     CDS             1631..2038
                     /locus_tag="SI621_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEI4065638.1"
                     /translation="MNRYALIKNNIVVNIVVWDGAGDMFADFKAVNISGVAVDIGWIY
                     DGNNFTAPPEPEKTKEQLIQEAELEKEGRIRAANDFINSKQWPGKAAIGRLKDEELAQ
                     YNLWLDYLDALDLVDTSSAPDIEWPTPPAVQAS"
     gene            complement(2010..2243)
                     /locus_tag="SI621_002949"
                     /pseudo
     CDS             complement(2010..2243)
                     /locus_tag="SI621_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2158..2544
                     /locus_tag="SI621_002950"
                     /pseudo
     CDS             2158..2544
                     /locus_tag="SI621_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3009..3665
                     /gene="pphA"
                     /locus_tag="SI621_002951"
     CDS             3009..3665
                     /gene="pphA"
                     /locus_tag="SI621_002951"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HEI4065639.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHIIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGVVFGGELTVVQLQ
                     "
     gene            complement(3666..3857)
                     /locus_tag="SI621_002952"
     CDS             complement(3666..3857)
                     /locus_tag="SI621_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HEI4065640.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(3962..4198)
                     /locus_tag="SI621_002953"
     CDS             complement(3962..4198)
                     /locus_tag="SI621_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HEI4065641.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(4316..5755)
                     /gene="rsmF"
                     /locus_tag="SI621_002954"
     CDS             complement(4316..5755)
                     /gene="rsmF"
                     /locus_tag="SI621_002954"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HEI4065642.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTASYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTAQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(5835..8468)
                     /gene="yebT"
                     /locus_tag="SI621_002955"
     CDS             complement(5835..8468)
                     /gene="yebT"
                     /locus_tag="SI621_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HEI4065643.1"
                     /translation="MSQKTPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(8437..9720)
                     /gene="yebS"
                     /locus_tag="SI621_002956"
     CDS             complement(8437..9720)
                     /gene="yebS"
                     /locus_tag="SI621_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HEI4065644.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESENARFDD"
     gene            9850..10347
                     /gene="msrC"
                     /locus_tag="SI621_002957"
     CDS             9850..10347
                     /gene="msrC"
                     /locus_tag="SI621_002957"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HEI4065645.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     TSNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10444..11142
                     /gene="proQ"
                     /locus_tag="SI621_002958"
     CDS             10444..11142
                     /gene="proQ"
                     /locus_tag="SI621_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HEI4065646.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAVGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            11162..13210
                     /gene="prc"
                     /locus_tag="SI621_002959"
     CDS             11162..13210
                     /gene="prc"
                     /locus_tag="SI621_002959"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEI4065647.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            13402..14283
                     /gene="htpX"
                     /locus_tag="SI621_002960"
     CDS             13402..14283
                     /gene="htpX"
                     /locus_tag="SI621_002960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEI4065648.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(14329..15702)
                     /locus_tag="SI621_002961"
     CDS             complement(14329..15702)
                     /locus_tag="SI621_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065649.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            15879..16670
                     /gene="kdgR"
                     /locus_tag="SI621_002962"
     CDS             15879..16670
                     /gene="kdgR"
                     /locus_tag="SI621_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HEI4065650.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(16814..17053)
                     /locus_tag="SI621_002963"
     CDS             complement(16814..17053)
                     /locus_tag="SI621_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065651.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            17212..17355
                     /gene="mgrB"
                     /locus_tag="SI621_002964"
     CDS             17212..17355
                     /gene="mgrB"
                     /locus_tag="SI621_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HEI4065652.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            17430..17717
                     /locus_tag="SI621_002965"
     CDS             17430..17717
                     /locus_tag="SI621_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HEI4065653.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         4681 a         4210 c         4656 g         4321 t
ORIGIN      
        1 gccaccagag ggctgattac tgcatttgat attgactctg cggttggtgt gcaacttgac
       61 attctgggat tgtggatcgg acgtagccgt gttgtcagcc agcctatctc aggtgtctat
      121 ttcagctggg ataccgacgg gcttggatat gatcaggggg tatggcaggg gccatatgat
      181 cctgattccg gatacatgta tctcagcgat gaaacttatc gtgtcatcct taaagcgaag
      241 attgcgatta ataactggga cggacggaat gattcgcttc cagcaattca tgacacggcg
      301 acagcaggat ccgggctgcg aatgcagatt gtcgataacc aggacatgac gatatcggtc
      361 tgggtctttc ctgatactga tatttcagat gtatcgcgtg agttaattgc tgcaattaaa
      421 cagggatatc tcacagtaaa agccgccggg gtgtgggcgg gtggtattga aacaccttcg
      481 gtggaaaccc catcggaagg ttcaaaattt tttggttttg atatggataa cgaatttatc
      541 agtggttttg atgtaggggc atggggagta ttactctgat ggcaaaaaat gactttaaag
      601 cattcgcaac gggtaaaaat gccaatgtta tgtcgcagaa ggaatgggaa gcgttgcctg
      661 cgcttttatc cggatttaca gcagggaaag cctccagtgc gcaagtcaat aaggttattc
      721 ggcaggccag ctttattgct gcagctctgg cccagtttgt aagtgataaa acgcaacggg
      781 atgtgcttga taatggtgat ctgcccggtt ttgttgaatt gctgggatcg gggtttgctg
      841 ttgaatacct gagccgcaag aatccgtttg gtgatatcaa atcggatggc acggtgaaaa
      901 cggctctcga aaaccttggt ttgggagatg gaacaggaag actcattgca gtgtaccgga
      961 ttgacacatc ccggcaaatt acactgccga aagatgccag gtttgttgtg gcagatttga
     1021 ttggtggcgg aggcggcggt ggcagtaact ccacgaccgt acaacccgga tatgcgtcag
     1081 caggacaggg cgggaattca ggtagccgtg tggtagcaaa attcacagtt gatgccagtc
     1141 agaaaattaa tattactatt ggcgcaggtg gtgcgggggg cgtgccaact cccgcaaccg
     1201 gagctaatgg aagtgacggt ggtgctacga ttatcgacgg tattactgct actggtggga
     1261 gaggtggagc taagggggca gcatttaatg ttctgccaac cggcgggcct acagcgtctc
     1321 cgaatgctaa taacaccatt cctggtagag cagaattgat agcacagcat atcgggtatg
     1381 gtggaaactc tggatttctg ttctcggata aaaatgcgct tggcgggaaa ggggctgact
     1441 ctgagttcgg agtgggcggt gtggcaatcg gaattagttc gggagtcaat gcatcggcgg
     1501 gaaataatgc cagaggcaat ggctctggcg gaagcggcgc ggtctctatt tatggctcag
     1561 ggtctaccgg ggtgtctatg tcaggtggag caggggctga cggtctggcg attttgtggg
     1621 tatacggcta atgaacagat acgcactaat taaaaacaat attgttgtta atatcgttgt
     1681 ttgggatggt gcaggagata tgtttgccga ttttaaggca gttaatatct ctggtgttgc
     1741 agttgatatt ggatggatct atgacggaaa taatttcaca gcccctcccg aacctgagaa
     1801 aactaaagaa cagttaatcc aggaagcaga actcgaaaaa gaaggacgca tacgtgctgc
     1861 aaatgatttt attaacagca agcagtggcc cggtaaagcg gctattggtc gtctgaaaga
     1921 cgaggaactg gcgcaatata atttgtggct ggattacctg gacgcactgg atctggtgga
     1981 tacctccagt gcgccagata ttgaatggcc tacgcctccg gcagttcagg ccagctaatc
     2041 tcaggtgccg tcgaagtgtc gacggctttt acttcttttt ttgtattcta tccaggcaga
     2101 tagtttttct ttatctgtac tgctgatatc accaagcatt aattccacaa ttccacccgc
     2161 cagtcagttg tgacattatc ggcaggcgac acactgggtg tctggaatgt cgtgctggtg
     2221 gaggagttgc gcgaacgagg catcaacttt cgtagtctga ctgattcaat tgataccagc
     2281 acaccaatgg gacgcttttt cttccatgtg atgggtgccc tggctgaaat ggaacgtgaa
     2341 ctgattgttg aacgaacaaa agcaggactg gaagctgctc gcgcacaggg acgaattggg
     2401 ggacgtcgtc ccaaacttac accagaacaa tgggcgcagg ccgggcgatt aattgcatca
     2461 ggcgttcctc gccagaaggt ggcgatcatc tatgatgttg gcgtatcgac tttgtataag
     2521 aagtttccgg tcggagataa atgaaaccgt agcacgtcgt atgcaagaac gtgccacggc
     2581 tggctgatgg acgttcgata gcgcgagttt gaatgaaaat cagccggaga tgattttaca
     2641 taattgctac ggaattattc aatacaggaa ttgcttgtgt atgcatggat tgacctgaaa
     2701 tattcccgaa aatttctcta aaaaactcga aaaaaatggt aactaattga atgtattaat
     2761 atgcaatggt acgcgttagg gattaaaaga tgaacgtaaa tttattcaac gcattaattt
     2821 taaaggggtt tattgtttgt tgacgaaaac aggaatcgtg ttcggtctct ttttatctgt
     2881 taaaagccag aagcattccc ttcgctgact ttatagtcaa ccataacaca cactctactg
     2941 tctgagtcca gcgtttttta acattcttgt taagattatg tgatctttag cgcgggagga
     3001 aaatattgat gaaacagcct gcgcccgttt atcagagaat tgcgggtcat caatggcgac
     3061 atatctggct ttcaggcgat atacacggtt gtcttgagca gttgcgccgc aaattatggc
     3121 attgtggttt tgatccgtgg cgagatttac ttatctcagt gggagacgtt atcgatcgtg
     3181 ggccgcaaag tttacgttgc ctgcagttac tggaacaaca ttgggttcgt gcggtaagag
     3241 gcaatcatga acagatggcg atggatgcgc tggcatccca gcagatgtct ttatggttga
     3301 tgaatggcgg cgactggttt attgcgctgg cagataatca acagaaacaa gcgaaaacgg
     3361 cgctggaaaa atgtcagcat ttgcccttta ttcttgaagt acacagtcgc accggcaagc
     3421 atattattgc tcatgccgat tatccagatg atgtttatga atggcaaaag gacgtcgatt
     3481 tgcatcaggt cttgtggagc cgctcgcgat taagtgaacg ccaaaaagtg cagggaatta
     3541 caggtgctga tcatttctgg tttggtcata caccgttgcg ccatcgcgtg gatattggca
     3601 acctgcatta tattgataca ggtgttgtct ttgggggcga actgactgtt gtgcaattgc
     3661 aataattaaa aatcaccgta ctcctgtgca ggtcgccaga aaccatctat aaaatcctca
     3721 atcggaaaac aaccgccatg gcggatccgt tgatcgctca tagaataaag acactgctgt
     3781 tccgtgttgt agacatccac aacaatatct tcacaaccgc catccaggta gcaaacaaaa
     3841 agtaccagcg cgaacatttc atccccgaag tgtggtgccg taccgttaag tttaggagag
     3901 attttacaac gggggaataa ccaggacaaa taacccgcca tgccgacggg ttctttttgg
     3961 attaggcaag acgcataatc caggcatcag attgttgatc gtaacgggta cggtaaagga
     4021 cagagtgttc gccatttagc agggcaggga tactggtttc agcatcccag gcatccagtt
     4081 gcatacataa atcggggtca gatttacaag gaatggttaa cgttcgatca cccggcgatt
     4141 cgccgtgtaa ttcgccgtcg tcgatttcaa aagcgcctat gcgcacactg gttttcatct
     4201 tcgtgctctc catttagctg ccgattgtgg tacgaccagt ccgatcgcac catttttcag
     4261 cctagttgag gcagccgaat gtgccagtca aaaagtgcgc tttttttgtg cgctgtcagg
     4321 cgttaccggt aaaaagtttc ccatcgcgca ccagttcacg cgggtagctg tttttcagtc
     4381 gcgaaccaat tcgtttggct aaaccaatcg gctgatgctg gaaagtaacc aatacatcat
     4441 ccgctactgg cgcggcttgc gggtaaacat cgcgcccgcg ataccactcc tccgcttcct
     4501 gcgccgttag ttcaaaagcg ttcacattgt cgggtgaggc aagggcaata accgcttcat
     4561 gctgccagcg ataacctttg ttatgcgttt cggcaagttt aatccccagg cgagaaaatc
     4621 ggactttacc gatcagggct tcaatgccca ccgggaacaa ccacagttct ttgtcacgct
     4681 gccagaggcg caggttttca tcccagttta agccaacacc tgcagccgcc tgacgaattt
     4741 gtccagcttc gcgatctttc accgggctaa acgggaaatt gcccactttg tattttgggg
     4801 cgggtaagac ggggatcgcc tgagttttac gcagacgagc aacgaagaag ccttcgcagt
     4861 cgtaaatttg tgggaaaaca tgcaaaaagc cttcttcggt cagcgcctta tttgcaccag
     4921 ggaagagatc gccaagcggt aaaaactcta ctgcatcggg gtaagtctct ttaagccacc
     4981 ggcagacggc ttcattttct tcctggttta aggtacaggt cgagtaaacc agcgtaccgc
     5041 cagggcgtaa tgcatgaaag gcgctgtcga tcagttcccg ttgggtagct gcgatttcct
     5101 gattgctttc tggtgaccag ttttttagcg catcgggatc tttacgcacc acgccttcgc
     5161 cggagcaggg agcgtccagc aaaatggcat cgaacatttc tggcactgcc gcaccaaata
     5221 cacggccatc aaaatgcgtg agcgcaacat tacggatgcc acagcggctg atattggcat
     5281 gtaacacttt tacccgactg gcggaaaact cattggcaag gatagcccct tcgttattca
     5341 tccgcgcggc aatttgcgtc gttttggagc ctggcgcagc agcgacatcc atcacccgct
     5401 gtggtgcatt accgtcagca aacaaggcgg caacgggcaa cattgaactg gcttcctgaa
     5461 tataaaacag gccgcttaaa tgctcggcgg tactacccaa tggcaatgca tcttcatcgt
     5521 cgcgttcaat ccagaaacct tcttcacacc acggaattgg cgtaagcgtc cagccataag
     5581 aggcggttaa ttgcaggaaa tcagcaacgg agattttcag cgtattaacg cgaatgctgc
     5641 ggcgcaacgg gcgctgacag gcggcaagaa aatcatcaaa agagagcgtc gaaggcatcg
     5701 cttcgcgcat ttgcgtcaga aaggcgtccg ggaaataaac ggtgtgttgg gccacgagca
     5761 tataccactg gcaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc
     5821 cggagcagtg ggcattattt gggaagcgca gtaccccatt cacgccactc tttcggttca
     5881 ctttcctgca acaggaagtg cttgccttcc tgggctttcg gtgccagtgg cgtacctgga
     5941 ggcgtggcga aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg
     6001 ccgcccgtca gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta
     6061 cgcaccaggt gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat
     6121 gtccccagcg tcattccggt aaccgtaccg acttccagac cacggaacag cacaggcgta
     6181 ccgatgccta acgaaccggc ttccggcgct tcaacaataa tgcttaagcc atccaggtaa
     6241 cgcgaatcag ttatggtggc ctcttgtaat tcaaagtcgc ggcgaggatt gccccggcct
     6301 ggttcgacgt tgatatacgg ctggaggata gtatcaagat gctcaacgcc agctgccgaa
     6361 atttgcggtg tgaccactga gaagcgtgta ccaccgcgag caaaggtctg aacatattcc
     6421 ggatagagca ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg
     6481 atttgcccga tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca
     6541 tcgaaagcgt gaagcgtaat ctgcccgcca accgcacggg ccgctgtttc ggaagcatac
     6601 agaatacgct tgtcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta
     6661 atagccccct ttaatgctct ggagagcggg gatgcctgta cagtcagacc actaccattc
     6721 agctgaactt tcgccccacc ttctgcccag aacacgctgt tgctggtcag aaggttgcga
     6781 tactccggct taatatgcag atcgatatca aacgcgttag ctcgcggacg cacggtaata
     6841 acttcaccaa cttcaaattt acggtacagc actaccgatc ctgcctgcac atccggcagc
     6901 gtctctgcac tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct
     6961 ttttccagat tggcatacag tggatagctg gctttcatct cgcctttatc gcctggcaga
     7021 atacgtatcc cgccgttaat ccattctgag gcgctggcac caagaaactc aacgccatcc
     7081 agccccacct tcacgtcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt
     7141 tcgcgatgct gaggctcgat ggcgacggta aaggtgacgc ctttgctggt gagtttacga
     7201 tcaataacct ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaatgccg
     7261 taactttctg gtgctgtcag ggtcagtgtc agaacatccg gctcttgtag cagggctttt
     7321 tcacctggca caacaacgaa ctctttgcgt ggctcgccat cgccgggcac caactcaaag
     7381 gtttttccgg tcagcagggc gctgagattg gcatcgctaa gggataattt cgggttgcgt
     7441 aattcgatgc gggtattttc acgaagcagg gtgacgacgc tgggatcaac ggtcatttcc
     7501 ccagtgactt taccaccagg atttaaatcc agtttagtca gctgtccgac ttccagcccc
     7561 tgatacatta acggcgtcga gtcggcggtt aatccggccc cactcggcag ttccagtttt
     7621 attattacgc cacgctggct gtgggcgaga tcttcataca gaccaaaggt atcttccgcc
     7681 tcggcaggtt tcgactcttc tggtgaatcg aaggcaatcg caccgttaac cagtgccgcc
     7741 agactttcca gtttcacctt cgcgccactg atactgacgt tggcatcaac gccggaaacg
     7801 ttccagaaac ggctaccttt tttcaccaga tcggtaaaac gccgctcgat caggacatca
     7861 atcaccacgc cttgcttatt gggattgatg gcatagtcgt agactttacc caccgggatc
     7921 ttacggaaat agaccaatga accactgttc agtgaaccga gatcgggggc ttgcaggtgg
     7981 atcatcagat cgccattatc cagccgatac ttcggttggg tatcgagtgc gacaaagtga
     8041 tcctgctctt tacctttacc tggcatcatg ccgatatagt taccaccgac gagggcatcc
     8101 agcccggaga cacctgccag cgaggccttt ggcgtcacca gccagaactg tgtctcttcg
     8161 cgcagcgcat ctttcatatc agacttgatg ctgaccttga cttcaatctt acgaagatcg
     8221 tcgctcaggc tgatatcctg cacagttccg acttcaacgc cctgataacg aacaggggtg
     8281 cggcccggga caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc
     8341 tgataactgt cccaaatcag ccaaccagca atcattagcg cgatgaacgg cagcagccag
     8401 aaaggtgaga tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgttttc
     8461 tgactcatgt gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa
     8521 aattaccgct gcgccgaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc
     8581 gcgattaatc agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgacc
     8641 aatccaggtc accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa
     8701 atgaatactc aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact
     8761 ggcgataaac acgattcccg caacggcaat gttgctactt gccagcgaca taattccgga
     8821 aagaattgta tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaacaggtt
     8881 ggcaggtaac aacaaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg
     8941 acggcgacgc aggcgtagcg ggatatggca acgcgggcag cgaccacgct gatcaggata
     9001 gccggtaaaa tggcacccaa gacagacacg gagtttctcg tccctacgcg tagcggggcg
     9061 ctgcggataa aatcgctccc acagttcttc gacattaaga tgtgacaacg tcaccgtcgt
     9121 taaaatcacc aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc
     9181 ctgtaccttt atagaagcaa cgccaatgcc gaccaggtag atatccagca ttacccactc
     9241 tttaagtcgt tcaagcatca gcagtaccgg acgtaaattc attcccagtc ggttaccaaa
     9301 ccataaataa gctatggagg tcaccagaat gaggggggca ccgataacgc agaaaaagac
     9361 catcgacccc gttatcgcat cgccctgttt ggtcatttgc cagatgcctt gcataacgtt
     9421 ggcgtcaata cgaatgccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg
     9481 cataagcaac agcatggtga aggccattgc cgccaggcgc gttagcgacc agtcgcgccc
     9541 gtcacgaatt tttgcctgac agcgcggaca ataggcactt tgatgagaat ttatctcggg
     9601 caggctaaat agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc
     9661 accgatagcc ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat
     9721 gacgtgccaa atgtaataag aattaacatt aatcttaacc catgatggga atgatcttga
     9781 gcattaacgc atttaatgct tattttaata ggctggcagt tcctggtaag acaaccaagt
     9841 gattatataa tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg
     9901 atggcaggag aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatatgag
     9961 cgtctcactg acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtgctc
    10021 ggaccatttc agggcaaaat tgcctgtgtc cggatacccg tcgggcgcgg cgtgtgcggc
    10081 actgcggttg cccgcaatca agtgcagcgt atcgaggatg ttcatgcgtt tgacgggcat
    10141 attgcctgtg atgcgacgag taattctgaa attgttctgc cgctggtggt gaaaaatcag
    10201 attattggtg ttctcgacat cgacagtacc gtcttcggtc gctttacaga cgaggacgag
    10261 caaggcttac gtcagcttgt ggcacagctt gaaaaggtgc ttgcaacgac ggattacaaa
    10321 aaattctttg cgagcgtcgc aggataatca acggataacg tagcatttac tgatagcgtc
    10381 attataatga cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat
    10441 ttcatggaaa atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa
    10501 cgttttcccc actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt
    10561 caggatttgg tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc
    10621 gctttacgtc tctacacttc gagctggcgt tatctttacg gtgttaaacc cggcgcaacg
    10681 cgtgtcgatc ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct
    10741 cgcaagcagc ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg
    10801 aaaaaacgcg aagctgccgc agctgttggt gagaaagaag acgcaccgcg acgcgaacgc
    10861 aagccacgtc cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa
    10921 aagccggtag agaaagcgcc aaaaacagta aaagcacctc gcgaagaaca gcacaccccg
    10981 gtttctgaca tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac
    11041 gcgatggatg ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg
    11101 ggtatgtctt tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg
    11161 catgaacatg ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac
    11221 cttcgctgta gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca
    11281 gcatgcgacg gtgagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt
    11341 cgacctcgat caggcatttt cggccaaaat ctttgaccgc tatctgaatc tgctcgatta
    11401 cagccacaac gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt
    11461 aggcgatgaa ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca
    11521 aaagcgtcgt tttgaacgtt accagtacgc tttgtcggta ctggaaaagc cgatggattt
    11581 caccggtaac gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc
    11641 tgagttgaac gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc
    11701 aggaaaaacg gataaagaaa ttcgtgaaac cctgactcgc cgctacaaat ttgccattcg
    11761 tcgtctggcg caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg
    11821 tgaaatcgac ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga
    11881 aatgagtttg tcgctggaag gtattggcgc agttctgcaa atggatgatg actacaccgt
    11941 tatcaattcg atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga
    12001 caaaattgtc ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct
    12061 tgatgatgtg gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt
    12121 acctgctggt aaagggacca agacccgtac tgtaacgttg acccgtgaac gtattcgcct
    12181 cgaagaccgt gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgttct
    12241 ggatattcca ggtttctatg tgggtttgac agacgatgtc aaagtgcagt tgcagaaact
    12301 ggaaaaacag aatgtcagca gtgtcatcat cgacctgcgt agcaatggcg gtggggcgtt
    12361 gaccgaagcg gtatcgctct ccggtctgtt cattccatcc ggtccgattg ttcaggtccg
    12421 tgataacaac ggtaaagttc gtgaagacag cgataccgat gggcaggttt tctataaagg
    12481 cccgctggtg gtgctggttg accgcttcag tgcttcggct tcagaaatct ttgccgcggc
    12541 aatgcaggat tacggtcgtg cgctggttgt gggtgaaccg acgtttggta aaggcaccgt
    12601 tcagcaatac cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc
    12661 gctgggttct gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca
    12721 acgtaaaggc gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaactgg
    12781 tgagaaattc gaagataacg cgctgccgtg ggatagcatc gatgccgcga cctatgtgaa
    12841 atcaggcgat ttaacggcct ttggaccgga gctgctgaag gaacataatg cgcgtatcgc
    12901 gaaagatcct gagttccaga acatcatgaa ggatatcgcg cgcttcaacg ccatgaagga
    12961 caagcgcaat atcgtttctc tgaattacgc tgtgcgtgag aaagagaata atgaagatga
    13021 tgcgacgcgt ctggcgcgtt tgaacgaacg ttttaaacgc gaaggtaaac cggagttgaa
    13081 gaaactggat gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt
    13141 gaatatcgca ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgctcc
    13201 cgtcaagtaa tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat
    13261 gccgtaaatc agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc
    13321 cagacttgaa aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta
    13381 aactgaggta aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc
    13441 gtaatggtcg ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag
    13501 gggctgatga tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg
    13561 tccaaatgga tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa
    13621 agggaacgtt ggctggtcaa tactgtagca acccaggctc gtcaggcggg gatcgctatg
    13681 ccgcaagtgg ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt
    13741 gatgcctctc tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc
    13801 gaggcggtaa ttgctcacga aatcagccac atcgccaatg gtgatatggt caccatgacg
    13861 ctgattcagg gcgtggtgaa caccttcgtt atctttattt cccgtattct ggcgcagctt
    13921 gccgcgggtt ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg
    13981 atctactttg cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc
    14041 accatgtggt tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt
    14101 ggtcgcgaga aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa
    14161 gcaaccagca tgatggcttt ctgtattaac ggtaagtcga aatcgctcag tgagttgttc
    14221 atgactcacc cgccgctgga taaacgtatt gaagctctgc gtacgggtga atacctgaag
    14281 taatatgaag taaatactaa aaagcgcgtc ctgatcgacg cgcttttttt atgccctggc
    14341 tcgtggctga gtgatacgta gaccactgac acaggcagca attactgcca gaatagccgc
    14401 agccatcagc gagacgtgcg taccattatc accaaactga tttagcatca gcgccaccag
    14461 cgccgcgcca ctactctgac ccaatagacg agccgtccct aacatgccac tggccccacc
    14521 gctacgttcg cgcggagcgg aggtaataat ggtgtggtta ttgggtgact gaaataagcc
    14581 aaatccagcg ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg
    14641 cagcagaacc agggaaaaaa gccccgcagc catgatgaac aaccctaaag ctcccagcaa
    14701 tcctgcatgt acgcgttcaa tcaaatagcc tgccagcgga gccatcacca ttgttgctaa
    14761 cggccacggt gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag
    14821 gtaaaagggc agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca
    14881 aatagaaagt gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtacgggaag
    14941 agaaagctgg cggcgaataa agaaaatacc aacgacaacc attaccacca gttccgcgcc
    15001 aatcaacgtc agcgattgcc cctgagcgaa accactcaat gcagtaataa gcaggccgaa
    15061 ggttaacgcg ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cacgagagcc
    15121 attgggtggc agaaaacgca tcgccagaag cagggcgata atacccaacg gtacgttgat
    15181 taaaaataac catttccagg atgcgatgga gaggattgct gcagcaattg tcggaccggc
    15241 agcagaagat acggcaacaa taaacgagtt tatgcccatc cctctaccca gaaaacgttg
    15301 tggatagatc aggcggataa gtgcggtatt aacgctcatc aacgctgcac cgccgaaacc
    15361 ttgtatgaca cgcgcaaggg tgagcatttg cagcgaatca gaaagggcgc agaacagtga
    15421 agacaacaga aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcgcc
    15481 cagaaacgaa aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca
    15541 gatggaactg gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc
    15601 gattgcgccg tcaaggacag ccatcgaaat accaatcaca atggttaata tcgcaccgta
    15661 tcgctggggc aatggcaggc cgtcggcctg aacttttggc ataggaattt tatatctttg
    15721 gtgaataatc aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt
    15781 gtaactaacc gacaaaagat ggattgcgac caccctggcg cgaaattata ataaaaaaca
    15841 gttctgattt ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata
    15901 aacagcctga ttctgtatct tccgtgctga aagtttttgg cattttgcag gcgctgggtg
    15961 aagagcgcga aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg
    16021 tttatcgctt tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg
    16081 agaaatattc cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg
    16141 atttaattcg tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta
    16201 tccacctcgg cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt
    16261 acaatttgcg catgtattca cggattgggc gtcgtaatcc gctgtacagc accgcgattg
    16321 gtaaggtact gctggcatgg cgcgatcgcg atgaagtgaa gcaaattctt gagggcgtgg
    16381 agtataaacg cagtaccgag cggaccatca ccagtacaga agcgttatta cccgttctgg
    16441 accaggtgcg tgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat
    16501 gcattgcggt tccggtattt gatcgctttg gcgtggtcat tgccggtctt agcatctcct
    16561 tcccgacgtt gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg
    16621 cggcgcgcaa aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag
    16681 aacctgtctg aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa
    16741 ggtggcgtta tattaaacgc acttgcgata agaatatttt actcatgagt gagaatctgg
    16801 ttatttatta cccttaacca ttatcgacca caatattgct tttacgtaac aacgggcaat
    16861 ctgttatccc cagaaaacca cttttagtgt gcaggtattg taccgtgctg gtgcctctgg
    16921 cggtcagata ggtacattgc aaacctaatc ccgcggcatt ctctttgctt ccaatcaaaa
    16981 cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa
    17041 tgatgaatcg cattacaacc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg
    17101 cgctgaagca agcgactcat tctgaaaaag cacgaatatc gacatagtta ggcgctgttt
    17161 aactaacgca tgctagttta atgacataag gtaggtgaaa cggagattgg agtgaaaaag
    17221 tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta
    17281 ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag
    17341 tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta
    17401 gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga
    17461 acattgcgtc tttggttgta tcggtggtgg ttctttttat cgggcttatc ttgtggtttt
    17521 ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag gcgttgctgg
    17581 atcagcaaaa acgtcaaaat gctctgttac gtcgtttgtg cgaagcaaac gaacctgaga
    17641 aagcagataa aaagaccgtt gagagtcaaa aatcggttga agacgaagat attattcgtc
    17701 tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc
    17761 aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa actctggtct
    17821 gacttcaaat aattatcata ttatttattt gcgtaacggg atatcaga
//
LOCUS       DARNVM010000097        17550 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.097, whole genome shotgun sequence.
ACCESSION   DARNVM010000097 DARNVM010000000
VERSION     DARNVM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.097"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(88..1116)
                     /gene="epmB"
                     /locus_tag="SI621_002966"
     CDS             complement(88..1116)
                     /gene="epmB"
                     /locus_tag="SI621_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEI4065654.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1158..1724
                     /gene="efp"
                     /locus_tag="SI621_002967"
     CDS             1158..1724
                     /gene="efp"
                     /locus_tag="SI621_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEI4065655.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1776..1901
                     /gene="ecnA"
                     /locus_tag="SI621_002968"
     CDS             1776..1901
                     /gene="ecnA"
                     /locus_tag="SI621_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HEI4065656.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2012..2158
                     /gene="ecnB"
                     /locus_tag="SI621_002969"
     CDS             2012..2158
                     /gene="ecnB"
                     /locus_tag="SI621_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HEI4065657.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2333..2650
                     /gene="sugE"
                     /locus_tag="SI621_002970"
     CDS             2333..2650
                     /gene="sugE"
                     /locus_tag="SI621_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEI4065658.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2647..3180)
                     /gene="blc"
                     /locus_tag="SI621_002971"
     CDS             complement(2647..3180)
                     /gene="blc"
                     /locus_tag="SI621_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HEI4065659.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(3268..4401)
                     /locus_tag="SI621_002972"
     CDS             complement(3268..4401)
                     /locus_tag="SI621_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HEI4065660.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4464..4823)
                     /gene="frdD"
                     /locus_tag="SI621_002973"
     CDS             complement(4464..4823)
                     /gene="frdD"
                     /locus_tag="SI621_002973"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEI4065661.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4834..5229)
                     /gene="frdC"
                     /locus_tag="SI621_002974"
     CDS             complement(4834..5229)
                     /gene="frdC"
                     /locus_tag="SI621_002974"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HEI4065662.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5240..5974)
                     /gene="frdB"
                     /locus_tag="SI621_002975"
     CDS             complement(5240..5974)
                     /gene="frdB"
                     /locus_tag="SI621_002975"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HEI4065663.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5967..7775)
                     /gene="frdA"
                     /locus_tag="SI621_002976"
     CDS             complement(5967..7775)
                     /gene="frdA"
                     /locus_tag="SI621_002976"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEI4065664.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8100..9077
                     /gene="epmA"
                     /locus_tag="SI621_002977"
     CDS             8100..9077
                     /gene="epmA"
                     /locus_tag="SI621_002977"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HEI4065665.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9296..10798
                     /gene="yjeM"
                     /locus_tag="SI621_002978"
     CDS             9296..10798
                     /gene="yjeM"
                     /locus_tag="SI621_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HEI4065666.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRITGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWVVE"
     gene            10896..11210
                     /locus_tag="SI621_002979"
     CDS             10896..11210
                     /locus_tag="SI621_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HEI4065667.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11239..14562)
                     /gene="mscM"
                     /locus_tag="SI621_002980"
     CDS             complement(11239..14562)
                     /gene="mscM"
                     /locus_tag="SI621_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HEI4065668.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14584..15552)
                     /gene="psd"
                     /locus_tag="SI621_002981"
     CDS             complement(14584..15552)
                     /gene="psd"
                     /locus_tag="SI621_002981"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEI4065669.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLSAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15649..16701)
                     /gene="rsgA"
                     /locus_tag="SI621_002982"
     CDS             complement(15649..16701)
                     /gene="rsgA"
                     /locus_tag="SI621_002982"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HEI4065670.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLRIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16796..17341
                     /gene="orn"
                     /locus_tag="SI621_002983"
     CDS             16796..17341
                     /gene="orn"
                     /locus_tag="SI621_002983"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEI4065671.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4014 a         4525 c         4629 g         4382 t
ORIGIN      
        1 tggttatata aaaagtaata acagcaatgt atatgaataa ttaaaggtta tgcaacgtgc
       61 aaagattaaa cttccctgtt taatcagtta ctgctggcgt agctggagat ccagcggcgt
      121 tttgctgggt tcgccgccaa tttctcgcgc cagtttaggc accagatatc ccgacaccag
      181 tgtcagcaac tcacgcataa tctgccgtgc ttcgtcatca ctcaccataa aatgcgccgc
      241 gccctgtacc ttatcgagca catgcaggta atacggcatc acgccggcat cgaacaacgc
      301 attactcagg tttgccagcg tttgtgcgtt atcgttcacg ccacgtaaca gaacgctctg
      361 gttgagcaaa gtaacgccta cccggcgcaa cttagccatc gcctgacgga atgtttcatc
      421 tatctcattg gcatggttga tgtgattcac cagcaagatt tgcagcgtag aatgtgaaaa
      481 gcgttcaacc agcgcatctg tgatgcgtgc cgggatcaca atcggcagac ggctgtgaat
      541 ccgcagacgt ttgatatgcg ggatggcttc cagttgtgtg agcaaccagt ccagctcgtg
      601 atctttcgcc atcagcggat cgccgccgga gaaaatcatc tcgtccagtt ccggatgcgc
      661 agcaacatac tcaagtgcag tttgccagtt acgcttgttg ccctgatttt cggcataggg
      721 gaagtggcga cggaagcaat agcggcaatt taccgcgcag ccgcctttga ccagcaaaag
      781 cgcccggttg tggtatttat gcaacaaacc aggcactacg ctgtgctgtt cttccagtgg
      841 gtcggtggag aatccgggcg cgacgacaaa ctcatcttgc gaggtaagta cctgacgcaa
      901 aagaggatcg tccggattgc ctttctccat gcgatcgata aatgagcggg gcacacgcag
      961 ggcaaaaagc tttttggcgc tgcgtccggc caacagtttt tcgtccgcgt ctatattcaa
     1021 aagacgcaga agttcatcag gatcggtcac aacatcggca agttgcgtta accaatcttc
     1081 tctggatggg gtatttaggg ttacaatatg cgccattttg tggcttagct accaattaac
     1141 aaatttcaga gggccttatg gcaacgtact atagcaacga ttttcgtgct ggtcttaaaa
     1201 tcatgttaga cggcgaacct tacgcggttg aagcgagtga attcgtaaaa ccgggtaaag
     1261 gccaggcatt tgctcgcgtt aaactgcgtc gtctgctgac cggtactcgc gtagaaaaaa
     1321 ccttcaaatc tactgattcc gctgaaggcg ctgatgttgt cgatatgaac ctgacttacc
     1381 tgtacaacga cggtgagttc tggcacttca tgaacaacga aactttcgag cagctgtctg
     1441 ctgatgcaaa agcaattggc gacaacgcga aatggctgct ggatcaggca gagtgtatcg
     1501 taactctgtg gaatggtcag ccgatctccg ttactccgcc gaacttcgtt gaactggaaa
     1561 tcgtggatac cgatccgggc ctgaaaggtg ataccgcagg tactggcggc aaaccggcta
     1621 ccctgtctac tggcgctgtg gttaaagttc cgctgttcgt gcagatcggc gaagtcatca
     1681 aagtggatac ccgctctggt gaatacgtct ctcgcgtgaa gtaatgcggt tgtggtgcgg
     1741 cctgcaggct gcaccatcac ttattcaggc cagagatgat gaaacgcctt atcgttcttg
     1801 ttttgcttgc cagtgcgctg ctcacgggct gtaataccgc tcgcggtttt ggtgaagaca
     1861 ttaaacatct cggtaattcc atctcccgcg ctgccagcta atttttcttc tcttccgaaa
     1921 aatcatcaga tttccatcat ttttggtgat gttgtctatt attaatttgc tataggcaaa
     1981 cataaataac attacctaaa aggaagacgt tatggtgaag aagacaattg cagcgatctt
     2041 ttctgttctg gtgctttcaa cagtattaac tgcctgcaac accacgcgtg gcgttggtga
     2101 agacatttct gatggcggta acgcgatttc tggcgcagca acgaaagcgc agcagtaagc
     2161 aataacggta cgacagttat gtcgtgccgt ttgttttttt gcgatagtca caaaggtaat
     2221 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2281 cctgcaaacg cctcttttca ccggggacgg ccccaattct ccggagcctg atatgtcctg
     2341 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
     2401 ccacggcttt agtcgtttga cgccaagtgt tattactgtg acggcgatga ttgtcagtat
     2461 ggcgctactt gcctgggcga tgaaatcgtt accagtaggg acggcttatg ccgtgtggac
     2521 gggtattggc gcagtcggcg cggccatcac cggcattgtg ctgctcggcg agtccgctaa
     2581 cccgatgcgc ctggcgagtc tggcgttaat cgtattgggg attattggtc tgaaactcag
     2641 cactcattaa ctaccgggtt gctgtaccca aataaactta ctgatatcaa acccttcccg
     2701 ggtcgcgatt gccagcatct cctgtttcac ttcgtcagaa atggttggcg tgcgagagag
     2761 tatccacagg tagtcgcggt ccgggccgca aaccagcgcg tggcggtact cccggtcgag
     2821 cgcgatgaca ttataaccgc cgtagaatgg cccaaagaac gacactttca gggctgcgcg
     2881 acgcggatct ccagtaaagt aggcttttcc ttcactctgc tgccacatct ctctgtccgg
     2941 gttatagcct ttattaatga cattcaggcc gccgtcatcc cgcaggctat acgtcgcggt
     3001 cactttctcc agcccgcgtt caaaacggtg atcaaaacgg gcaatctcat accatgtacc
     3061 cagatagcgt ttggtatcga aattatttac cacggtcacg ccacgcggtg gtgtgggaga
     3121 actacaggca accaccagaa atgcagctgt cgctgcggca acgagaggga gcaggcgcat
     3181 aaatgtttcc ttactggttt tttctaagtg tagatgacag caaggaaaag cgcagaaaag
     3241 gtccgaaaat tcggacccga tgaaatttta ctgtagagcg ttgagaatct gccaggcggc
     3301 ggcgactctc gctggattgg gatagttttt gttagccagc atcacgatac ccagctcttt
     3361 ttctggaata aacgcaacat agctaccaaa tccgccggtc gcccccgttt tatgtaccca
     3421 tgatgcgcgc actgcaggag ctgggggcgt aatcgctttt acggggcgtg ctgccagtgc
     3481 aattttattg tcactgccgt taatgatgat gtcaggattt accggccagt ccagcatttc
     3541 ccagcccagg ccctgataca tatcgccggt ttgccagtag cgagattgtg ccagttgtat
     3601 cccttgttga agcgttttct cagtgatatc aaggggtttt aaattgcttt gcacccagcg
     3661 ggccatatct tcaatggtcg acttcacacc ataagcttca gcatctaacg cccctggcga
     3721 aacatgcact gccttacctt cgcgatatcc ccaggcgtaa ttcttttctt ctgcggacgg
     3781 cacattaatc cacgtatgag tgagtttgag tggctggaag acacgagttt gcatcgcctg
     3841 ctcaaaactc aaaccagacg gcttcacagc cagtgcgccg aacaaaccga tactggagtt
     3901 ggcatacaga cgttgtgttc ctggagccca tgcaggttgc cagttttgat agaagcgcag
     3961 caagtcgctt gaggatttca cctcatccgg cacctgcaat ggcaggccgc cagcggtgta
     4021 ggttgcgaga tgtaatagtg tgatcccatt ccattgttta gcggtaagtt caggccagta
     4081 ttttgtcgcg ggatcgctta acttgatttc ccctcgtgca atagcgtcgc caccaagtac
     4141 gcccgtaaat gttttgctga ccgagcctaa ctcaaacaac gtttgctgtg tgacgggctg
     4201 ctttttggcg atgtccgcat agccccaggt aaagtaataa ggtttaccct gataaattac
     4261 cgccaccgcc ataccgggga tcttttgttg ctctataagc ggggttattg tgcgatgcac
     4321 aatatcgttg atttgttgag gggcagcaaa tgtggagcaa gaggcggtaa ttaataaggc
     4381 gcagagcgtc attttgaaca tagggtctgg tttccataca aaacggcccg ccataggcgg
     4441 gccggattta cattggcgat gcgttagatt gtaacgacac caatcagcgt gacaactgtc
     4501 aggatagcag ccagaccgta gaaaacccat ttgcccgcag gtacgtggat tttcagatcg
     4561 tgcatcgcgt ggtgcatacg gtgtaaacca caccacagcg gcagaacgat catcaggaac
     4621 aggaatacgc gaccaatgaa gctctgcgcg aacgccagaa cgcgctcgta gctcagtgca
     4681 tcacccggaa acagccccag cggcagcaga atacccacca gcaggatcat caccggcgca
     4741 ataattgcgc tccacatacc accggccccg aagaggcccc agaataccgg ttcgtcagaa
     4801 cgctttggat ttggattaat catctcaggc tccttaccag tacagggcaa caaacaggat
     4861 tacgatggtg gcaaccacag ttaccgccca gagacttttg ataattggct ctggtcccat
     4921 tttttcgtct tttacaatga tattggccgc tttcggtgcc agttcaaacc aggttttggt
     4981 gtgcagcaga gctgccgcca gagtgatcag gttaatgatc acaataaccg ggttttgtaa
     5041 aaagtcgacg aatcccgccc agctttccgg gccatttttc agggcaaaca gcccgaaaat
     5101 cagttcaatg ctgaaccaca cagccggaac cgccgtgcct tcgcgcagca tgtaaaagcg
     5161 ataaaacggc aattttttcc accaggtgga cgtcattggc cgtacatacg gtttacgttt
     5221 agtcgtcatg ttgcactcct tagcgtggtt tcagggtcgc gataagaaaa tctttcgaac
     5281 tttctacttt gccctgctga atggccgcag ccggatcgac gtgtttcggg cagacttcgg
     5341 agcagtagcc cacgaaagta cagctccata cgccgttctg gctgttcaac tgcgccatac
     5401 gctccttctt accgtggtcg cggctatctt cgttgtaacg atgcgccagc gtaatggcag
     5461 ccggaccgat gaactctggg ttcaggccaa actgcgggca cgcggcgtag cacagaccac
     5521 agttgatgca accggaaaac tggtgatact tggccatctg cgccggggtc tggatgttag
     5581 taccctgatc cgcggtgcgg gagttgccga tgatgtacgg tttgatcgct tccagacttt
     5641 cgatgaagtg ggtcatatcg accaccagat cgcgttcaat cgggaagtta gctaacgctt
     5701 caaccttcat accgtcggtg taatcacgca ggaaggtttt acatgccagt tttggcacgt
     5761 tgttaaccat catgccgcag gaaccacaaa tcgccatacg gcaggaccag cggtagctca
     5821 ggtccggtgc caggttgtct ttgatgtagc ccagcgcatc cagtaatgag gtagttgcgt
     5881 cataaggcac ttcatagaat gcgctatgcg gtgcggtatc gacttccggg ttatagcgca
     5941 ccacctcaat tttcaggttt ttcatctcag ccattcgcct tctccttctt attggctgct
     6001 tccgccttat cggctgcatc cgcttcgcca ccgtaaacac gtttagctgg cggcagcgta
     6061 gtaatcttca cgtcgctgta ctccaggcga gtcgtgccat cagcatcgcg gaaggcgagg
     6121 gtgtgtttga ggaagttgac gtcgtcacgc tcggtgcaac cttcgtccag acgctggtgt
     6181 gcgccgcggg actctttacg tgccattgcg gagtgcgcca tacattcagc aacgttcaga
     6241 ccgtggccca gttcaatggt gtagagcagg tcggtgttga acacgctgga ggtgtcggtg
     6301 atgcgcacgc gcttgaagcg ttcctgcagc tcagccagct tgtcgatggt tttctgcatc
     6361 agttccggcg tacggtagat accgcaacct tcttccatag ccaggcccat ttcgtcgcgg
     6421 atcttcgccc agttttcgcc gccatcctgg ttaaccagat ctttcagacg ttgttcaacg
     6481 ccagctgcct gcgcttcaat tgccgcttcg ttgccattac cggcagttgc tgcacgctct
     6541 gtcgcttgtt caccggccag acggccgaag accaccagtt ccgccaggga gttggagccc
     6601 agacggtttg caccgtgtag accaacagag gaacattcac ccacggcgaa cagaccttta
     6661 atgcgggttt cacagttctg atcggtttcg ataccgccca tggtgtagtg tgcggtcgga
     6721 cgtaccggaa tcggttcttt aaccggatcg acgccaacgt acgctttcgc cagttcgcag
     6781 atgaacggca gacgttcatg cagttttttc tcgccgaggt gacgcaggtc gagataaacc
     6841 acatcgccac gcggcgtgga gatggtgttg cctttacgcc attcgtgcca gaaggcctga
     6901 gaaactttgt cgcgtggacc cagttccata tatttgtttt tcggctcgcc cagcggagtt
     6961 tccgggccca tgccgtaatc ttgcagataa cggtagccat ttttgttgac cagaataccg
     7021 ccttcaccgc ggcagccttc ggtcatcagg ataccggaac ctggcagacc ggttgggtga
     7081 tactgaacga attccatgtc acgcagcgga acgccgtggc ttagcgccat acccataccg
     7141 tcaccggtaa cgatgccgcc gttggtgttg taacgataaa cgcgacccgc accgccggta
     7201 gccataacga ccgcgttagc acggatctgc accagcgtgc cttccatcat gttcattgct
     7261 accaggccgc gaacatgacc atcatcaacc agaatatcca gcacgaaatg ttcgtcaaaa
     7321 cgctggatct gcgggaattg cagagaggtc tggaacagcg tgtgcagcat atggaagccg
     7381 gtcttatcgg cggcgaacca ggtacgctcg attttcatgc cgccgaagcg acgtacgttg
     7441 acgctaccat ccgggcgacg gctccatggg cacccccaca gttccagttg ggtcatttcg
     7501 gttgggcagt ggtggacgaa ataatccacg acatcctgct cacacaacca gtcgccaccc
     7561 gctactgtat cgtgaaagtg atattcgaag ctgtcatgat cctgcgcgac agcggcggag
     7621 cccccttctg cagcaacggt atggctacgc atcgggtata cttttgagat tagtgcgatt
     7681 tttgcatttg gatttgcctg cgcggcagca attgcagcac gtaatcccgc gccaccggcg
     7741 cctacaatgg caagatcggc ttgaaaggtt tgcacgacat tcctccagat tgtttttatc
     7801 ccacagcccc gtacttcagg gtaagtgcct gaaagttacg gtctgcgaac gctattccac
     7861 tgctccttta taggtacaac agtatagtct gatggataag tctgaaattt gacgagatcg
     7921 atttttttag tgcgcaagag ggctaaatta tcactgaaga tgattaattt aattactaaa
     7981 ccatcagatc gtgctttttt tagtcacagc ccacccttgt ttgattcctg cgtaaaattt
     8041 gtctcgccgt cgcgttgcga gtagacttcg tgcccttgtc aaaaactgga gatttaacta
     8101 tgagcgaaac ggcatcctgg cagccgagcg catccattcc taacttatta aaacgcgcgg
     8161 cgattatggc ggagatccgt cgtttctttg ccgatcgtgg agtgctggag gtggagacgc
     8221 cttgtatgag ccaggcgacg gtaaccgata ttcatttggt cccgtttgag acacgtttcg
     8281 ttggccccgg gcattcgcag gggatgaatc tctggttaat gaccagcccg gaataccata
     8341 tgaagcgcct gctggttgcc ggttgtgggc cggtattcca gctgtgccgc agcttccgta
     8401 atgaagagat ggggcgttat cacaaccctg agttcactat gcttgagtgg tatcgaccgc
     8461 actatgatat gtaccggttg atgaacgagg tggacgatct cttacaacaa gtgctggact
     8521 gcccggcagc agaaagcctt tcttatcaac aagctttctt gcgttatctg gaaattgacc
     8581 cgctctctgc cgacaaaacg caactgcggg aagtggcagc gaaactggat ttgagcaacg
     8641 ttgcagatac cgaagaagac cgcgacacgc ttctacaatt gctgtttacc tttggcgtag
     8701 agccaaatat tggtaaagaa aaaccgacct ttgtgtacca ctttccagcc agccaggcat
     8761 cactggcgca aatcagtacc gaagatcatc gggtcgctga acgctttgag gtttattata
     8821 aaggtattga gctggcgaat ggtttccatg aattgacgga tgcccgtgag cagcaacaac
     8881 gctttgaaca agataaccgt aagcgcgcgg cgcgcggttt gccgcagcac cccattgacc
     8941 agaatctgat tgaagccttg aaagtcggta tgcctgactg ttccggcgtg gcattaggcg
     9001 ttgatcgtct ggtgatgttg gcgctgggcg cggagacact ggctgaagtc atcgccttta
     9061 gcgttgaccg ggcataattc tgaaaaatta ctgaattaac cgcgaagaat ggcgtgataa
     9121 gcgccattct gtagcatatg ttttcatttt tccgctaaag gccacaattt aagactggat
     9181 atttgatatc atccaggtat caattctgta ttggttttta cgctgcccgc tcaatgttgg
     9241 gcagtgcagt tcagtgggcg cgttttatac acgcgctgaa atgaaggatg gtttcatgcc
     9301 tcacacgata aaaaagatga gtctgatagg actcatattg atgatcttta cttccgtatt
     9361 tggatttgcc aatagcccat cggcttatta cttaatgggt tatagtgcga ttccctttta
     9421 tatattttct gcattgttat tctttattcc attcgcctta atgatggctg aaatgggagc
     9481 tgcttatcgc aaagaagaag gtggtatcta ttcctggatg aataatagtg tcggaccacg
     9541 ttttgccttc attggtacat ttatgtggtt ttcctcttat atcatctgga tggtgagtac
     9601 ctccgcgaaa gtttgggtac cgttctcaac attcctctat ggtagcgaca tgacccagca
     9661 ctggcgtatt accggactgg agcctacgca ggtggttggt ctgctggcag tggcatggat
     9721 gattctggtc accgtcgttg cttcaaaggg gattaataaa attgcccgca ttactgcggt
     9781 gggcggtatt gcagtaatgt gtctgaattt agtattgctg ttagtaagca ttactatttt
     9841 gttattaaat ggtgggcatt tcgcgcagga tattaatttc cttgcatcac cgaacccagg
     9901 ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt gctatttttg cctatggcgg
     9961 aattgaagcg gttggtggtc tggtcgataa aacggaaaat ccagaaaaga actttgccaa
    10021 aggtattgtt tttgccgcta ttgttatttc aatcggttat tcgctggcaa tatttttatg
    10081 gggcgtcagc acaaactggc agcaggtatt aagtaatggt tccgttaacc tcggcaatat
    10141 tacctatgtg ctgatgaaga gcctcggggt gacgctgggt aacgcactgc atttgtcacc
    10201 agaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt accggacttt cgatgttcct
    10261 cgcttatacc ggtgcgttct ttacgctttg ctattcaccg ctgaaagcca tcatccaggg
    10321 gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg ggatgccgtc
    10381 tatcgccatg tggatgcagt gcgggttggt tactgtcttc atcctgctgg tttcgtttgg
    10441 tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg tgtctatgac
    10501 gcttccttac ctgttcctcg cgctggcttt cccattcttt aaagcacgtc aggatctcga
    10561 cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt
    10621 tctggtggtg acatttgcga acgtcttcac catcattcaa cctgtggttg aagccggaga
    10681 ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc
    10741 gatttaccag aactattgca gcagagtggc aaaaaatccg cagtgggtgg tggaataacc
    10801 cgatgcccta tcgtctgtct gacgataggg cgcaatgaca gggaattagt gggatttcgt
    10861 atgtgctata gcgaaattaa actacgggtt atctcatgag tgcgcgcatg tttgtcttgt
    10921 gctgcatctg gttcattgtg gcgttcctct ggataaccat tacctccgcg ctggacaaag
    10981 agtggatgat tgatggacgc gggattaaca acgtctgtga tgttttgatg tatctcgaag
    11041 atgacgatac tcgcgatgtc ggtgtgataa tgacgttacc gctctttttc ccttttctct
    11101 ggttcgcttt gtggcgaaaa aaacgcggct ggtttatgta cgccaccgcg ctggctattt
    11161 tcggctactg gctgtggcag ttttttctgc gctatcagtt ttgtttgtga gccggatggg
    11221 ttcatccggc acaaaacctt acaaacttcc cgcctgacga cctttgcccg cagaagttaa
    11281 tgttctcccc gtttgtttac cattcaggct ttccagacgc atctggaagg gcgggaatgg
    11341 catatcgata ccgtgggcat ggaagcctgc cagaattagc tggtggatct catggcgtag
    11401 cggcatacgg tgacccatct cagcggcgta aatacgcagc tcgaaaatct gaatcccctg
    11461 ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg agcagcgacg
    11521 cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcggcag gggccggaat
    11581 cgtcaacacc acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat
    11641 aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg tggtggcgcg
    11701 ggtgttaatt ttcgtcacgc taccagtaag atcgcggatc gttaccgtat cgccaatgcg
    11761 aatcggtttt tcgaacagga tgatcaggcc agagataaag ttggcgaaaa tttcctgcaa
    11821 accaaaaccg agaccaacac cgagcgcggc gaccagccac tgcaatttcg accactcaat
    11881 accaatcatt gagaagccga ccagcccgcc aatcagcatc agcagatatt tggtgatggt
    11941 ggtgatggcg taacctgtac ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag
    12001 cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa tggcaatcag
    12061 caccgcaccg agggtaatcg gctccagact ttctacgccc tgtaccgtgg aggtgacatc
    12121 ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat
    12181 gaccgaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg actgcgcact
    12241 gatggcatcg agatcgactt cgctttcatc gacttcaatt gccccttccg ggctactgtg
    12301 atgatgtgct tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc ggcgatgctt
    12361 cgcccggtca aacgccagcc tgcggcgttg gatcagcatc cagcggcgga taacgtgata
    12421 aaccaccagt agcaaaaacc agatggcaac cgaggtttca agcctcgcta acagcgcctg
    12481 cgccgttgcc agataaccca ccgccgacgc cagaatggca accagcggtg cgccaatcat
    12541 catgttccac agcatatggt tggtaatgtt gtcaccgctg ccttctttgt tgagatacag
    12601 cgggatcccg gcctttttca gactgagagt gaccaccgcc agcgcaccgc aaatgaggat
    12661 aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat cgaacatcat
    12721 cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc gcatcccacg
    12781 ggaaacccgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg ggcgggcaaa
    12841 ggtggcgcaa atcatcacta cccacagcag cggcacggtg gccgttacgc catcaccaat
    12901 cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg tcatccacag
    12961 caccggtagc ggtgacgcga cgagaatcga ccagaaaaga gtgcgtaacg tcagccagaa
    13021 gtgatcctga gtcactttgc cgactttcgc cgccgaacgt tcaagaaaac gggtgaaata
    13081 gcggcgtgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg gtaaaattgt
    13141 ctctttactg gtcagcatca tcacactggc tttgcccaac tgactgaagg tgtccagcga
    13201 aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca tcgggcgcac
    13261 gtcagaggtc cagaacagat agcggtgcgt cgcttcattc acctctttca gcgcatcctc
    13321 cagttgcccg ttggagactt tcagcttggt cagttccagt agtagcgtgt ctccaccctg
    13381 caacaatgag ttcagcaact cacgctgagt gcgtagctgt gcttccagaa tacggttttg
    13441 ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagctgcg gctgtttatt
    13501 aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg tatcaagctg
    13561 ttgtggtttt ggcatttccg gcagccgtgc cacctgcgcc cgcaacgctt cgccgagcag
    13621 attggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgctt gccggacctg
    13681 taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc gctgcgcctg
    13741 ttgattcagc gccgctgata gttcgcggtt aattttgaat tgcgcgacga tgtctttcgg
    13801 caaatcggcg ctgttttctg ccagcaattc ggtactttcc agcgcccgct ccgcctcaag
    13861 ctgacgttgg ctgttcaact gattacgtaa ggcctgcaaa tacgcatcca gttgctggct
    13921 ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat tggcggacag
    13981 ctgcgccagt tccagttcat cgacgagcgc tttcaggcgt gcggagtcag actgcaacgc
    14041 gaaattttgt gcctgattga gcggggaatt gccggtaagc gtccccaggc ggcgctcgat
    14101 ttcatttaac tgacggcggg catcggtttg ctgttgcggc agttgattca gcgaatcggc
    14161 aatctcgcgg gcgcgttcct gctcttgctg ggcctgacgg cttttatcca gcaactggct
    14221 gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggtg acacgctgcg
    14281 cggctcgtca cgcatgttgt ttaattgcgc gcgcagagta gcggagagtt tcggataatt
    14341 atcaatgact tcttgatatt gcttgatgcg ctcaagggaa cctttccgtt cctcaagcgc
    14401 atttaaggca gactggagcg cctctacgac ttccggttgc gcgggtttcg ccgcttttgc
    14461 ctgctccagt tcttgtgaga tttgtttgct atcgggggcc gtcgcggcgt acgcccccca
    14521 actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat
    14581 ggattagacc tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt ctgcttcgat
    14641 ctcttcagca gaaagcgggg cgggttcagc gtctggcgta acaaaggttt cggtagatac
    14701 tgccagcggc tggccaattt tcgtaacaga caggctttcc agttgctcaa ccagattcac
    14761 tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac ccatttcctg
    14821 gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat
    14881 gatgccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc tgcctacaat
    14941 cgtcgcaccg accagaatct gcgccatcgg gccaaattcg gtatcgaaca ggcagatcac
    15001 gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt taacggagaa
    15061 gagatcgcct ggcacgtaga tcatctcacg cagaataccg ttgcacggca tgtgtacgcg
    15121 gtggtagtca cgtggggaga ggtaagtggt cacaaacgta ccgttacgga agagatccgc
    15181 catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt tggcttgcag
    15241 gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag gcataaccag
    15301 tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga caaagaattc
    15361 gttaaaggtg cggtagctgg cggtatccgg cttttgcgcc tctttcatgt cgaccttgta
    15421 gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct tgcttgcgcc
    15481 ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg aaagtttaaa
    15541 tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg gcctacatgc
    15601 cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc agtcatccgt
    15661 atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac ggtgatagtt
    15721 ttcgaaacgg gtttccgcga tttttccttc ctcaaccgct tcccggatag cgcagcccgg
    15781 atcggtatcg tgtttgcaat cgcgatattt acacagacct aagtagtcat ggaattcgac
    15841 aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac gcactcctgg
    15901 ggagtcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg tcgtggtgtg
    15961 ctgaccgaga cccgagttgt cagagacatc gttggtcagg atctcttttt gcagccccag
    16021 caacgcgttc agcaggctgg atttaccgac gccagactgc ccggcaaaaa tgctgatgcg
    16081 cccggtcaac gcctcttcca gcggtttcag cccatcctga gtatggctgg aaaccatcaa
    16141 tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaatg ccatgccttc
    16201 gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatccgca aggtttcgca
    16261 ggccaccagg taacggtcga taatattgag cgacagctcc ggcaaaatgg cggagacaat
    16321 gacaatctgg tcgatgttgg cggcaatagg tttcacgccg tcgtagaaat ccggacgcgt
    16381 caacaccgag gtgcgctcat gcaccgcttc cacgatccct ttgacattta cgccttccgc
    16441 cgccggttta cccggacgcc agactacgcg gtcgccggtc accagcgaac ggatggtacg
    16501 gcgaatattg cagcggtgaa cgtcgccatc tgcggattcc acatcagcgt gcataccaaa
    16561 gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt
    16621 ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct gctggccttt
    16681 ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg gcaaaacatc
    16741 tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa atagcatgag
    16801 tgccaatgaa aacaacctga tttggatcga tcttgagatg accggtctgg atcccgagcg
    16861 cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata ttctggcaga
    16921 agggccgacc attgcagtac accagtctga tgaacagctg gcgctgatgg atgactggaa
    16981 cgtgcgcacc cataccgcca gcgggctggt ggagcgcgtg aaagcgagca caatgggcga
    17041 tcgtgaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg cgggaaaatc
    17101 gccgatttgc ggtaacagca tcggccagga tcgtcgcttc ctgtttaaat acatgccgga
    17161 gctggaagcc tacttccact accgttatct cgatgtcagc acgctgaaag agctggcgcg
    17221 ccgctggaag ccggaaattc ttgacggctt taccaagcag gggacgcatc aggcgatgga
    17281 tgatatccgt gagtcggtgg cggagctggc ttactaccgc gagcatttta tcaagctgta
    17341 aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat tttgacgcta
    17401 aattgaacgt cttgctgata atttcagcac ttgagataaa aactcaaaaa aaatttttta
    17461 ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga cgcagaaatg
    17521 cgaaaattac gaaagcaaaa ttaagtagta
//
LOCUS       DARNVM010000098        17468 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.098, whole genome shotgun sequence.
ACCESSION   DARNVM010000098 DARNVM010000000
VERSION     DARNVM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.098"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            56..1195
                     /gene="arnB"
                     /locus_tag="SI621_002984"
     CDS             56..1195
                     /gene="arnB"
                     /locus_tag="SI621_002984"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HEI4065672.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARLLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVNEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1199..2167
                     /gene="arnC"
                     /locus_tag="SI621_002985"
     CDS             1199..2167
                     /gene="arnC"
                     /locus_tag="SI621_002985"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HEI4065673.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2167..4149
                     /gene="arnA"
                     /locus_tag="SI621_002986"
     CDS             2167..4149
                     /gene="arnA"
                     /locus_tag="SI621_002986"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HEI4065674.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPEDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMHETIDETLDFFLRTVDLTDKPS"
     gene            4146..5036
                     /locus_tag="SI621_002987"
     CDS             4146..5036
                     /locus_tag="SI621_002987"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HEI4065675.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTLVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGMPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5036..6688
                     /gene="arnT"
                     /locus_tag="SI621_002988"
     CDS             5036..6688
                     /gene="arnT"
                     /locus_tag="SI621_002988"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HEI4065676.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVASQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6685..7020
                     /gene="arnE"
                     /locus_tag="SI621_002989"
     CDS             6685..7020
                     /gene="arnE"
                     /locus_tag="SI621_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HEI4065677.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7020..7406
                     /gene="arnF"
                     /locus_tag="SI621_002990"
     CDS             7020..7406
                     /gene="arnF"
                     /locus_tag="SI621_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HEI4065678.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7400..7666)
                     /gene="pmrD"
                     /locus_tag="SI621_002991"
     CDS             complement(7400..7666)
                     /gene="pmrD"
                     /locus_tag="SI621_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HEI4065679.1"
                     /translation="MEWLVKKSCCDKQDNRHVLKLCDAGGTIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7776..9131)
                     /gene="menE"
                     /locus_tag="SI621_002992"
     CDS             complement(7776..9131)
                     /gene="menE"
                     /locus_tag="SI621_002992"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEI4065680.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQDVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVALVNDEGWYAT
                     RDRGELHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9128..10090)
                     /gene="menC"
                     /locus_tag="SI621_002993"
     CDS             complement(9128..10090)
                     /gene="menC"
                     /locus_tag="SI621_002993"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEI4065681.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPRKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVEVDALERLL"
     gene            complement(10090..10947)
                     /gene="menB"
                     /locus_tag="SI621_002994"
     CDS             complement(10090..10947)
                     /gene="menB"
                     /locus_tag="SI621_002994"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEI4065682.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10962..11720)
                     /gene="menH"
                     /locus_tag="SI621_002995"
     CDS             complement(10962..11720)
                     /gene="menH"
                     /locus_tag="SI621_002995"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEI4065683.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFANYSRLY
                     VDLPGHGGSAAIRVDGFDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVIVEGGHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11717..13387)
                     /gene="menD"
                     /locus_tag="SI621_002996"
     CDS             complement(11717..13387)
                     /gene="menD"
                     /locus_tag="SI621_002996"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEI4065684.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWHDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTNGAQTLQQLLAQVSHL"
     gene            complement(13476..14771)
                     /gene="menF"
                     /locus_tag="SI621_002997"
     CDS             complement(13476..14771)
                     /gene="menF"
                     /locus_tag="SI621_002997"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HEI4065685.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPKHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQRLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14850..15155)
                     /gene="elaB"
                     /locus_tag="SI621_002998"
     CDS             complement(14850..15155)
                     /gene="elaB"
                     /locus_tag="SI621_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HEI4065686.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15210..15671)
                     /locus_tag="SI621_002999"
     CDS             complement(15210..15671)
                     /locus_tag="SI621_002999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEI4065687.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMNKTLET
                     CTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15736..16653
                     /gene="rbn"
                     /locus_tag="SI621_003000"
     CDS             15736..16653
                     /gene="rbn"
                     /locus_tag="SI621_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HEI4065688.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIVQPLTIYGPQGIREFVE
                     TTLRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16844..>17468
                     /locus_tag="SI621_003001"
     CDS             16844..>17468
                     /locus_tag="SI621_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HEI4065689.1"
                     /translation="MVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATKH
                     PLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
                     KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALND"
BASE COUNT         4106 a         4710 c         4408 g         4244 t
ORIGIN      
        1 acactcttta tagcaaatat aagtggacag gtattcaatg gcggaaggaa aagcaatgtc
       61 agaatttttg cctttttcgc gaccagcaat gggcgtggag gaactcgctg cagttaaaga
      121 ggttctcgaa tccggttgga tcacaaccgg tccgaagaat caggcgcttg aacaagcctt
      181 ttgccagttg acgggaaatc agcatgccat cgcggtcagt tcagccaccg ccggaatgca
      241 tatcacgcta atggcgttgg aaattggcaa gggcgatgaa gtgattacgc cttccctgac
      301 ctgggtttca accctcaata tgatttcctt gttgggtgca acgccggtaa tggtggatgt
      361 cgaccgcgat acgctgatgg tcacgcctga agcgatcgag tcagccatta cgccacgcac
      421 taaagccatc attccggtgc attatgccgg tgcgccagca gatattgacg ccattcgcgc
      481 cattggcgaa cgttacggca tcgcagttat cgaagatgct gcccatgccg tcggtacgta
      541 ttacaaaggg cgacatattg gcgcaaaagg taccgctatt ttttcgtttc acgccatcaa
      601 aaatattacc tgtgcggaag gtggcctgat tgttacagat aatgaaaacc ttgcccgcct
      661 gctacggatg ctgaaatttc acggtctggg tgtcgatgcc tatgacagac aaacctgggg
      721 ccgtgcaccg caagctgaag tcttaacgcc gggctataag tataatctga ccgatattaa
      781 cgccgcgatt gccctgaccc agttagccaa attagagcac ctcaacaccc gtcggcgcga
      841 aattgctctg caatatcagc aagcactggc agctctcccc tttcagccat taagccttcc
      901 cgcctggccg cacgttcacg cctggcatct gtttattatt cgtgtcaatg aacaacgttg
      961 tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa agaggcattg gtaccgggtt
     1021 acatttccgc gccgctcaca cacaaaaata ttatcgcgag cgttttccca cgctgtcgtt
     1081 accgaatacc gaatggaata gcgaacgcat ctgttcgttg ccgctgttcc cggatatgac
     1141 caccgccgat gccgaccgcg tcatcacagc ccttcagcaa ctcgcaggac aataagccat
     1201 gtttgaaatc caccctgtta agaaagtctc ggtggttatt cccgtttata acgagcagga
     1261 aagcttaccg gaattaatca ggcgcaccac aaaagcctgt gaatcactgg ggaaagagta
     1321 tgagatcctg ctgattgatg acggcagtag cgataattcc gcgcatatgc tggtcgaagc
     1381 ctcacaagcg gagaacagcc atattgtgtc tattttgctt aaccgcaatt acgggcaaca
     1441 ttcagcgatt atggcgggat tcagtcacgt tactggcgac ttaattatta cccttgatgc
     1501 cgatctccag aatccgccag aggagatccc ccgcctggtg gcaaaagccg atgaaggcta
     1561 cgacgtggta ggaactgtgc gtcagaaccg ccaggacagc tggtttcgta aaacggcatc
     1621 gaagatgatt aaccggctta ttcagcgcac caccggcaaa gcgatgggtg actacggttg
     1681 tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc atgaacgcag
     1741 cacctttatc ccgattctgg cgaatatctt cgcccgccgt gccattgaaa ttccagtgca
     1801 ccatgccgag cgtgagtttg gtgaatccaa atacagtttt atgcgcctga ttaatttgat
     1861 gtacgacctg gtgacctgcc ttaccacaac gccgctacgt atgctgagtc tgcttggcag
     1921 cattattgcg attggaggtt ttagcattgc ggtgctgctg gtgattttac gcctgacctt
     1981 cggaccacaa tgggcggcag aaggcgtctt tatgctattt gccgtgctgt ttacttttat
     2041 tggcgctcag tttatcggca tgggattgct cggtgaatat atcggcagga tctacaccga
     2101 tgtccgcgcc cgcccccgct attttgttca gcaagttatc cgtccatcca gcaaggaaaa
     2161 tgaataatga aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg tattgaagcc
     2221 ctgctgtctg ccggttacga aattagcgcc atttttaccc ataccgataa tcccggtgaa
     2281 aaagcctttt atggttcggt ggctcgtctg gcggcggaaa gaggcattcc ggtttatgcg
     2341 ccggataacg ttaatcatcc gctgtgggtg gaacgtattg cccaactctc accagaggtg
     2401 attttctctt tttattatcg ccatcttatt tgcgacgaga ttttgcagct cgctcccgca
     2461 ggtgcattta atctgcatgg ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac
     2521 tgggtgctgg tgaacggtga aacggaaact ggcgttacat tgcaccgaat ggtgaaacgt
     2581 gccgatgccg gggccattgt ggcgcaactg cgcattgcca ttgcgccaga cgatatcgct
     2641 attacgctgc atcataaatt gtgccatgcc gcgcgccagc tactggaaca gacattaccc
     2701 gccattaaac acggtaatat tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt
     2761 ggtcgcagaa caccggaaga cagtttcctc gaatggcata aaccggcatc cgtgctgcac
     2821 aacatggtgc gtgccgttgc cgatccgtgg ccgggtgcct tcagctatgt tggtaatcag
     2881 aaatttaccg tctggtcgtc acgcgtgcat tctcatgcgc ccgcagcaca accggggagc
     2941 gtgatttctg ttgcgccact gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc
     3001 ggacaggcgg gcgacggcat tactatgcag ggctcgcaat tagcgcagac gctgggcctg
     3061 gtgcaaggtt cacgcttgaa tagccagcct gcctgtgccg cccgacgccg tacccgggta
     3121 ctcatcctcg gggtgaatgg ctttattggc aaccatctga cagaacgcct gctgcgcgaa
     3181 gatcattatg aagtttacgg tctggatatc ggcagcgatg cgataagccg ttttctgaat
     3241 catccgcatt ttcactttgt cgaaggcgat atcagtattc attccgaatg gattgagtat
     3301 cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga tagccacgcc gattgaatat
     3361 acccgcaacc cactgcgcgt atttgaactc gattttgaag agaatctgcg cattatccgc
     3421 tactgcgtga agtaccgtaa gcgaatcatc ttcccgtcga cttcagaagt ttatgggatg
     3481 tgtagcgata aatacttcga tgaggatcat tctaatttaa tcgtcggccc ggtgaataaa
     3541 ccacgctgga tttattcggt gtctaaacaa ttacttgatc gggttatctg ggcctatggc
     3601 gaaaaagagg gtttacagtt taccctcttc cgcccgttta actggatggg accacgactg
     3661 gataacctta atgcggcacg aatcggcagc tcccgcgcta ttacgcaact cattctcaat
     3721 ctggtagaag gttcaccaat taagctaatt gatggcggaa aacaaaaacg ctgctttact
     3781 gatatccggg atggtatcga ggcgttgtac cgcattatcg aaaatgcggg aaatcgctgc
     3841 gacggcgaaa ttatcaacat tggcaatcct gagaacgaag cgagcattga ggaactgggc
     3901 gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc atcacttccc accgtttgcg
     3961 ggctttcgtg ttgtcgaaag tagcagctac tacggcaaag gatatcagga cgtagagcat
     4021 cgtaaaccga gcatccgcaa tgcccgccgc tgccttgact gggaacccaa aattgatatg
     4081 catgaaacca tcgacgaaac gctagatttc ttcctgcgca ccgttgatct tacggataaa
     4141 ccatcatgac caaagtcggc ttacgcattg atgtcgatac ctttcgtggc acccgtgaag
     4201 gcgtgccgcg tctgctggaa atcttgagta agcataatat tcaggccagc atttttttca
     4261 gcgtcggccc ggacaatatg ggccgccatc tctggcgact ggtgaagcca cagtttttgt
     4321 ggaagatgct gcgctcaaac gcggcatcgc tttatggctg ggatatttta ctggcaggta
     4381 cggcctggcc gggtaaagag attggtcatg ccaatgccga tatcattcgt gaagcagcta
     4441 aacatcacga agtcggcctg cacgcctggg atcaccatgc ctggcaagcc catagcggta
     4501 actgggatcg gcaaacattg gtcgacgata ttgcacgcgg ccttcgcacc ctggaagaga
     4561 tcataggtca accggtaacc tgttctgccg ctgcgggctg gcgtgccgac cagcaggtga
     4621 tcgaagcaaa agaagcgttc catttgcgct acaacagcga ttgtcgtggg gccatgccgt
     4681 ttcggccact gctcgaatca ggaaaccctg gcactgcgca aattccggtg accttaccca
     4741 cctgggatga agtgattggt cgggatgtga aagcagaaga ttttaacggt tggttactca
     4801 accgcatcct gcgagataaa ggcatgccgg tttataccat tcatgcagaa gttgaaggct
     4861 gcgcttatca gcataatttt gtggatctcc tgaaacgggc agcccaggaa ggcgtgacat
     4921 tttgcccttt aagcgaactg ttatcaggga cgttgccgct cggacaagtt gttcgcggaa
     4981 atattgccgg acgtgaaggc tggctgggtt gccaacaaat tgcgggtagt cgttgatgaa
     5041 atcggtacgt taccttatcg gcctcttcgc gtttattgcc tgctattacc tgttaccgat
     5101 cagcacgcgt ctgctatggc aacccgatga aacgcgttat gcggaaatca gtcgggaaat
     5161 gctggcatcc ggcgactgga ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc
     5221 cattgccgga tactggatta acagcattgg gcaatggcta tttggcgcga ataactttgg
     5281 tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc gcgctggtga cctggtttac
     5341 tctgcgctta tggcgcgata aacgtctggc tctactcgcc gcagtgattt atctctcatt
     5401 gtttattgtc tatgccattg gcacttatgc cgtgctcgat ccgtttattg cattctggct
     5461 ggtagcggga atgtgcagct tctggctggc aatgcaggca cagacgtgga aaggcaaaag
     5521 cgcgggattt ttactgctgg gaatcacttg cggcatgggg gtgatgacca aaggttttct
     5581 cgcccttgcc gtgccggtat taagcgtgct tccatgggtt gcatcgcaaa aacgctggaa
     5641 agatctcttt atttacggct ggctggcggt tatcagttgc gtactgacgg ttctcccctg
     5701 gggactggcg atagcgcagc gggagcctga cttctggcac tattttttct gggttgagca
     5761 tattcaacgc tttgcaatgg atgatgctca gcatagagct ccgttctggt actacttgcc
     5821 ggtcatcatt gccggtagcc tgccgtggct gggattactc cccggagcac tgtacgcagg
     5881 ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg agctggacga taatgccgct
     5941 gctgtttttc tccgtcgcta aaggcaaatt gcccacctat attctttcct gctttgcacc
     6001 tctggcaatg ctgatggcgc attacgcttt gctggcagca aaaaataatc ctctggcgct
     6061 gcggattaat ggctggatta acatcgcttt tggtgtcact ggcattattg ccacatttgt
     6121 ggtctcccca tggggaccaa tgaacacgcc agtgtggcaa accttcgaga gctataaagt
     6181 cttttgtgcc tggtcgattt tttcgctatg ggcatttttc ggctggtaca ccttaacaaa
     6241 cgtcgaaaag acctggtctt ttgccgcgct ttgcccgctg gggctggcgt tgctggtagg
     6301 attttcaatt cctgacagag tcatggaagg aaaacatccg caattttttg tcgagatgac
     6361 acaagaatca cttcagccaa gccgctatat tcttactgat agcgtcggtg ttgccgcagg
     6421 tctggcatgg agcctgcaac gcgatgacat catcatgtat cgccagacag gtgagttgaa
     6481 atacggcctt aattatccgg atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa
     6541 ctggcttaat caacatcgtc aggaggggat tattacactc gtgctttcgg ttgaccgcaa
     6601 tgaagatatc aacagtctcg ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct
     6661 ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat tagtctttgc cagcttactt
     6721 agcgttgccg ggcagttgtg tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg
     6781 cgcaaacata tcgtgctgtg gctgggactg gcgctggctt gtcttggtct tgccatggtg
     6841 ctctggctgc tggtcttgca gaacgtaccg gtaggcattg cctacccgat gttaagtctg
     6901 aattttgtct gggtgacgct ggctgcagta aaactgtggc acgaaccggt atcgccgcgt
     6961 cactggtgtg gggtggcgtt cattattggt ggcattgtga tcctcgggag taccgtgtaa
     7021 tgggcctgat gtggggatta ttcagcgtca ttatcgcctc cgctgcgcaa ttaagcctgg
     7081 gttttgcggc gagtcatctg ccgccgatga cgcacttatg ggattttatc gcggcgctgt
     7141 tagcttttgg cctggatgcc cggatactgt tactcgggct gctgggttat ctgctttccg
     7201 ttttttgttg gtataaaaca ctacataaac ttgcactaag caaagcctat gccttgctca
     7261 gtatgagtta tgtgctggta tggattgctt caatggtact tcccggctgg gaagggactt
     7321 tttcgctgaa agcactactg ggagtagcct gtattatgag cgggttgatg ctgattttcc
     7381 tgcccacgac aaaacaacgt tactgagttt tccctgccac tttacactgc cgttcccact
     7441 catcggggct ataactgctt gccgagagga ctttcaccgt atgcagcttt tcacgattaa
     7501 tacaataaag cgcattctgc aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac
     7561 tcttcacttc ggcgatcatt tttatcgtgc caccagcatc acaaagctta agtacatgtc
     7621 tgttatcttg tttatcacag catgactttt taaccagcca ttccattgca ccaccctgct
     7681 tgataagaat tttcacatta atttacagat taacagataa acctattatc aacggcaatg
     7741 gaatggcaaa attgcgaagt gaaactaatg atttcttaat cctggcgttg cacccactct
     7801 tttagcgcct gacgtgaaat cttaatgccg ccgtttttca gttcaggcgg cagcgttaac
     7861 caacgcaccg gttgctgaaa acgggccagc ttatctttca cccattcact aagatcaacg
     7921 gtttgctggt catactccac caccgctacc ggccgatgac caaactcctt gtcggcaacg
     7981 gggacgataa acacctgcaa aaccgcagga tgtgcagcaa taacgcgctc gacttcttcc
     8041 ggctgaatac cctcaccgcc actgaagaac agattgtcta aacgcccgac aatggtcagc
     8101 ttgccattat gcagctcacc gcggtcgcgc gtagcgtacc agccttcgtc attaaccagt
     8161 gccaccagtt gcccgttacg ccagtaacct tcagccatac tggcagcccg cagccacact
     8221 tcatcattaa cgattttcac ttcccgaccc ggcagcggcg aaccaacgtc tgccagaccg
     8281 tcggcttctt tcgcacacac cgtggaggca aactcggtca gaccatagcc gcaaaagcaa
     8341 cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc gccgccaagt
     8401 agcaccgctt tcagggaaac ggaactacgg ttaaccagca aacgccagag ttgtgttggc
     8461 accagtgaag cgtgagtaca gcctgccagc atttgctcca atggctgttt atcacgtacc
     8521 gtcatccgcg caccagcgta taaccagcgc cacataattc cctgaccgga gacgtgaaac
     8581 agcggtaaag agagcaacca gtcatcgtgc tcgccaaacg gtatcagcga taacacacct
     8641 tgcgcactgg caagatgggc ctgataagta tgaacagcgg ctttcggcaa accggtagag
     8701 ccggaggtca acgtcattga gcacagacgc gtcggctgcc acgcagcggc atgtgcgcct
     8761 tcaaccagct gaatgtgcag cgacgttaac gccggaaacg tgttttcccc atccggcacc
     8821 agcgcaaatt gcagcgtcag attgggcagc aattcttcaa gcaacggttg cggcagctga
     8881 gggttcacgg gcagcacccg cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt
     8941 tgcggcgtat tccacgcccg caacatcacg ccgctgccct caacaacgtc ctgcgccgca
     9001 aagccggagg ctaattcatc gacgcgagca caaagctcgc gccagttaag ttgctcgtca
     9061 ttaagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca cggccagtca
     9121 gagaagatca taacagccgc tccagtgcat caacttccac taacggtagc gggctacccg
     9181 gccagcgacg aacctgctgc gcctgcatca gatccagcgt gtccagcccc ggaatggtgt
     9241 ctggcgttaa ccaggcggca atccgcgcca gttgcgttaa gcctaagctc gactcaatag
     9301 aagaactgat caccgccgtc agccccaacg cgtgcgccgc ctgcacctgc tcgcgtactt
     9361 tatccaggct gcccgtgagc gtgggcttga taaccaccgc gcgcacgccc tcttcagcta
     9421 caaaggcaaa atctggctcg cgcaggcttt catcccaggc aatggcaatg ccggtttcac
     9481 gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaaa gcaatgcggt
     9541 ggcgataatc cgggttaacg tatttggcaa actgctggcc tttccgcggc gtccaggcgc
     9601 ggttcgcgtc aagacgcaga tgcagatccg gaattgcctc cagcaacaga ttgaccacca
     9661 tgccgtcgcg cacggcttcg tacaatccca ctttgacctt cgccactttc tcgccaggca
     9721 tatcggcaag tttgaggatc agatcgtccg gatcgccatt acacagcggt gccgcacggt
     9781 agttggctgc ttgcggcaac gcatcggtca gttctgccaa tgcacagctt acgccaaagg
     9841 ccacggaagg catctgcggt agctcgcaat cgcctgccag ccagttattt acccaggcaa
     9901 gcagcacgct ttgcgcctct tcccaggttt cctggctgaa gcccggcagt ggggagatct
     9961 ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta cagcccgtcg cgggttttta
    10021 accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg tatacctgcg
    10081 cgctacgcat tacggattcc gtttgaattt gctgaagtca ggctgacgtt tctggttgaa
    10141 agcgttgcga ccttcctgac cttcttccgt catatagaac agcatggtgg cgttacctgc
    10201 cagctcctgc agacctgcct gaccgtcaca gtcggcgttc agtgcagctt tcaggcagcg
    10261 cagcgccatc gggctgtttt gcagcatttc gcggcaccaa cggacggttt ctttttccag
    10321 atccgccagc ggtaccacgg tgttcacaag gcccatatcc agcgcctgtt ttgcgtcgta
    10381 ctgacggcac aggaaccaga tttcacgcgc ttttttctgc ccgacgatgc gagccatgta
    10441 ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac cgaagatggc
    10501 attatctgcc gcgatagtca ggtcgcacat catgtgcaga acgtgaccgc cgccgatgga
    10561 gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac gctggaagtc
    10621 cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat caccacgcac
    10681 tttctggtca ccaccggagc agaacgcttt atcgcctgca ccagtcagaa tgatcacgcc
    10741 gatgttgtcg tcataacgcg catctgccag cgcctggatc atctctttta ccgtcagagg
    10801 acggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac cgtcggtgga
    10861 tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg tgccattcaa ccggtgcgta
    10921 aagcattgct tcatcaggat aaatcatatt gtgtccttta gtcagaaacg caagatctgc
    10981 gccagacttg cgattacgcc agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga
    11041 atgacatggc attcggcagc cagttccgcc gccagggcgc ggaatttgct gtcacgttca
    11101 ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa atcgggctgg
    11161 acggcgagag aagtcgcctc cagcatggcg gcaagcgttg cgccattatt gttgctgcgc
    11221 agcgcaacca gctcccggcg ttgttcgtca ttgagtgagg caaaaacagg ctgttgatac
    11281 cagtcagcaa ataccgccgt taacggttct gtgcgaaaac gctgcgccca ttggcgatcg
    11341 gaacgctgac gttccgtgcg ttgttcagca ttttgcaacc cagggtgccc gccttcgaca
    11401 ataaccccac aaagccccgg catcccctga caagccgcca tcatcgccac ccgtccgcca
    11461 agcgagtacc ccaccagcca gaagttaagg atgttgtaac taaccagagt tttgcgcagt
    11521 aagccggtga catcatcaaa tccatcgacg cgaatcgccg ccgaaccacc gtgacctggg
    11581 agatcaacat acaaccgcga gtagttggca aacacctcgc ccacttcttg ccattcatgg
    11641 caatcgccgg aaaaaccgtg caaaaacacc agccagggta aacctggttt accgtgtttt
    11701 gcctgcgcgt gcaggatcat aaatggctaa cctgcgccag cagttgctgg agggtttgcg
    11761 caccattggt gtcgttaacc accatttcaa ttaccgtggt ggtcggcgtg cgccaggcgt
    11821 cggcaagtgc cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca
    11881 tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcgcgctcgt
    11941 ttttcggcgt tggcaacagc gagaaaattt gcccgccatt gttgttcacc acaattaata
    12001 ccagcggcgc ggaaacctga cgtaataacg ccagcgcgtt gagatcgtaa agtgctgaga
    12061 gatcgcccac aatcgccagc gtcggtttgc cactggcccg ctgaacgcca gcagcagttg
    12121 agagcagccc gtcgataccg ctggcacctc ggttgctgta caccgggtaa cctgccggaa
    12181 gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt accgacaaac aattgcccct
    12241 gttcaggcaa atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac
    12301 gggcaataac cgcctgcatt gcctgttcag ccaggcgcgg gatttcaacg caccagggct
    12361 ggcgtttttc tgccggatgt tgctccagcc agtcggcaat attggcaatt aagcgacgtc
    12421 cgcgatggtg tgccggatca agtcgccctt caatgtcatc aacaatccag tactcttcgg
    12481 gttcacagct tgcctgccat tgcaggagcc gtttgcccgt caggctgctt cccagttgca
    12541 ccacaatttg cgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg
    12601 cacacggcag cggctgtccg gtttgcgaca gcacgtcacc gatcagcggc cagccaagag
    12661 tttgcgccca cagtgcgact tttttgccct cttccgcact catgcgcccg gcaaccacca
    12721 cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg
    12781 cttcacgcag ccacggtttg tcgtcatgcc accagtcgcc cagccgctgt tgccagctaa
    12841 gcccggtgtc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc
    12901 caccagcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa cgtgcgggga
    12961 tatcctgggt cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg aacattcccg
    13021 gctgacgaat cgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta
    13081 acaggatcag tttttctccg gttaacccag cttcaatcag tgcaggatag agatttgcta
    13141 ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca
    13201 gcgccagatg ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct
    13261 ccgccgccgc taacgttaac ggtgtagaac gcgagccagg ggcgatacag atgtgtctta
    13321 cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta
    13381 ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga
    13441 ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc
    13501 agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga accacggaca
    13561 atgcccgcgc cagcatatag tcgcacgaca ttgccactga tttttgctga gcgcagggaa
    13621 acgcagaatt cgctttgttg taaagagaga tagcccgcag aaccggcgta ccattctcgg
    13681 gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact
    13741 gctgccgttg gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgaagtc
    13801 cagatacagc ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca
    13861 tccagcgttt gggtatcggc ctgtaatcgt tggcagatat cttccaccac caacatgttc
    13921 tcacgctggt ttttatcatc cgccatcagc cactcaccca accgctgcgc ctgcttatca
    13981 tcaggatgat ttgctaccgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc
    14041 cgccatagcc gttccggtga agagccaaga aaagcatttt cgccatcaaa ggccatataa
    14101 aaatgatagc agttcagatt aagccgacga ctggcagcca tcatcgctgc cgcattgacc
    14161 ggacttgcga aatgcaggtc agttgcccga gcaagcacca ctttgtcgag ctcaccttcg
    14221 gcgatggttt tcgttgccag ttcgattaat tgcgtccacc ccgttttgtc cggccagtgt
    14281 tgttctcggg tggtggttaa atgtaaccca ggcaagggct tgatactcac cagtgtggcg
    14341 ataaattctt ttgccttaat cgcatcatgc tgaagggagc tttcgctgaa tagcgtcagc
    14401 ctcagcgtgg ctttaccgcc acagcgtcgc cattccaggc gtggtaaaag taaattgccc
    14461 tgcgacgggt caaacgcatt cagcccccag atgcgtaagt cggcgtgttt cgggtgctga
    14521 cgaagaaacg tttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca
    14581 gcttcttcat caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc
    14641 agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcaat aacccgaatg
    14701 ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta
    14761 agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg tactacaaaa
    14821 aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc
    14881 agcccaacgg ccgcgcccac accaattcct tgccagggtt tttcgtggac gtagtcatca
    14941 gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt
    15001 tttttcacat catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg
    15061 gcgggatcgc cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtca
    15121 tcatcgatac gtgtatcacc aaactgatta gacattctcg ttctccattt gcgtaaaacc
    15181 tgtcactaac tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca
    15241 atgtgtggga taccatcttc ttcatagacc tccgtcacag ggataaagcc aaaactctgg
    15301 tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag gtttatcagg ccagtgacgc
    15361 gtacatgttt cgagtgtttt attcatcagc tgctgcccca ctttttcgcc acgcaacgct
    15421 tcactaacaa tcacccgacc aataacgacc ggctcaagat catcatcact tttcagaatc
    15481 ctcgcatacg ccaccagttc atcatttttc caaccgagaa tatggcgatt atcccccgtc
    15541 aaatcatcac catcgatatc ctgataaggg caattctgct caacaacaaa caccgcgcaa
    15601 cgcagttgta ataaggcata aagctgggaa accgataatt cagagtgatg cagatcttgc
    15661 cattcaatca taatgctctc cttttagatg tatgccttat actagccgga atattttttg
    15721 aaacgtgatg aactcatgga attaattttt ttaggtactt cagccggtgt gccaacccgt
    15781 acgcgcaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag cggtctttgg
    15841 ttgtttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga
    15901 aaactggaca agatttttat cagtcacctt catggcgatc atctctttgg tttacccggc
    15961 ttgctgtgca gtcgttctat gtctggtatt gtccaaccct taacgattta tggtccgcaa
    16021 ggtatccgtg aatttgtgga gaccacgctg cggattagcg gctcatggac cgattatccg
    16081 ctggaaattg tcgaaattgg cgctggcgaa attttcgatg atggcctgcg caaagtgacc
    16141 gcttatccgc tggaacaccc actggaatgc tatggctatc gtattgaaga acatgataaa
    16201 ccgggtgcat taaatgccca ggcattaaaa gctgctggcg taccgcctgg cccactgttt
    16261 caggaattaa aagcgggcaa aacaatcatg ctggaagatg gaaggcagat taacggcgca
    16321 gattacttag ccgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc
    16381 tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg
    16441 gatataacca tggaagccaa agccaatagt cgcggccaca gctctacacg ccaggctgcg
    16501 acactggccc gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat
    16561 gatgacaaag gttgtcagca cctgttacgt gaatgcaggt caattttccc ggcgactgaa
    16621 ctggcgaatg atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga
    16681 aacttgccct gttatcgcaa cccgcgctac atatatcccc ccacacgggc atattgagcg
    16741 atttttgata tttattcatc tccttacgca ctatattcag agcaataagt tgcctctttt
    16801 tatcttcatt cagcacaacc tttgcaacta aaaggagcct atgatggtta cagttgtcag
    16861 caattattgt caattatctc aaacccaact ctgtcagaca tttgcagaaa aatttactgt
    16921 gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta tccggagatg aagaaagtat
    16981 tgagttactg cataacattg cgttaggtta tgatgaattt gggaaaaaag ctgaagatat
    17041 tctttaccat attgttagaa acccaacaaa tgagacccta tcgattatcc gacttattaa
    17101 aaatgcctgt ttaaaattat ataatttggc acataccgca accaaacacc ccctcaaatc
    17161 acatgattca gatgatctcc tgttcaaaaa actattctcc ccttcgaaat taatgacaat
    17221 tatcggtgag gacattcctc ttatatcaga aaaacagtcg ctttcaaagg tacttttaaa
    17281 tgataagaat aatgaactga gcgatggtac aaacttctgg gataaaaatc gtcaattaac
    17341 cacagatgaa atagcttgct atcttaaaaa gatagccgct aatgcaaaaa atactcaagt
    17401 caattatcct actgatttct acctccccaa ttccaacagc acttacctgg aagtcgctct
    17461 caatgata
//
LOCUS       DARNVM010000099        17380 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.099, whole genome shotgun sequence.
ACCESSION   DARNVM010000099 DARNVM010000000
VERSION     DARNVM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.099"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            75..476
                     /locus_tag="SI621_003002"
     CDS             75..476
                     /locus_tag="SI621_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HEI4065690.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNGENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGIYSVQHEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            495..1193
                     /locus_tag="SI621_003003"
     CDS             495..1193
                     /locus_tag="SI621_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEI4065691.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLVRLQRQQGE"
     gene            1244..1903
                     /locus_tag="SI621_003004"
     CDS             1244..1903
                     /locus_tag="SI621_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HEI4065692.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGENIDDIDDIKLFVYE
                     ESYGISKESHWRETINAKAMGAMTLNWQEKRWLRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1921..2319
                     /locus_tag="SI621_003005"
     CDS             1921..2319
                     /locus_tag="SI621_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEI4065693.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGCIFNAACMTW"
     gene            2329..2967
                     /locus_tag="SI621_003006"
     CDS             2329..2967
                     /locus_tag="SI621_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HEI4065694.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYVDV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2970..4133
                     /locus_tag="SI621_003007"
     CDS             2970..4133
                     /locus_tag="SI621_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEI4065695.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDTDQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKLQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4217..5842
                     /locus_tag="SI621_003008"
     CDS             4217..5842
                     /locus_tag="SI621_003008"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEI4065696.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIEQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFMEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLNTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5959..6234)
                     /gene="yjfN"
                     /locus_tag="SI621_003009"
     CDS             complement(5959..6234)
                     /gene="yjfN"
                     /locus_tag="SI621_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HEI4065697.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6383..6712)
                     /gene="bsmA"
                     /locus_tag="SI621_003010"
     CDS             complement(6383..6712)
                     /gene="bsmA"
                     /locus_tag="SI621_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HEI4065698.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6894..7643
                     /gene="yjfP"
                     /locus_tag="SI621_003011"
     CDS             6894..7643
                     /gene="yjfP"
                     /locus_tag="SI621_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEI4065699.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFRGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLADRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7640..8395)
                     /gene="ulaR"
                     /locus_tag="SI621_003012"
     CDS             complement(7640..8395)
                     /gene="ulaR"
                     /locus_tag="SI621_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HEI4065700.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8503..9567)
                     /gene="ulaG"
                     /locus_tag="SI621_003013"
     CDS             complement(8503..9567)
                     /gene="ulaG"
                     /locus_tag="SI621_003013"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEI4065701.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9922..11319
                     /gene="ulaA"
                     /locus_tag="SI621_003014"
     CDS             9922..11319
                     /gene="ulaA"
                     /locus_tag="SI621_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEI4065702.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11335..11640
                     /gene="ulaB"
                     /locus_tag="SI621_003015"
     CDS             11335..11640
                     /gene="ulaB"
                     /locus_tag="SI621_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HEI4065703.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11650..12114
                     /gene="ulaC"
                     /locus_tag="SI621_003016"
     CDS             11650..12114
                     /gene="ulaC"
                     /locus_tag="SI621_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HEI4065704.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12128..12778
                     /gene="ulaD"
                     /locus_tag="SI621_003017"
     CDS             12128..12778
                     /gene="ulaD"
                     /locus_tag="SI621_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEI4065705.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12788..13642
                     /gene="ulaE"
                     /locus_tag="SI621_003018"
     CDS             12788..13642
                     /gene="ulaE"
                     /locus_tag="SI621_003018"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HEI4065706.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13642..14328
                     /gene="ulaF"
                     /locus_tag="SI621_003019"
     CDS             13642..14328
                     /gene="ulaF"
                     /locus_tag="SI621_003019"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HEI4065707.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14457..14732)
                     /locus_tag="SI621_003020"
     CDS             complement(14457..14732)
                     /locus_tag="SI621_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4065708.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHPILIREPGSNGNMHASADIYR"
     gene            15059..15454
                     /gene="rpsF"
                     /locus_tag="SI621_003021"
     CDS             15059..15454
                     /gene="rpsF"
                     /locus_tag="SI621_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEI4065709.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15461..15775
                     /gene="priB"
                     /locus_tag="SI621_003022"
     CDS             15461..15775
                     /gene="priB"
                     /locus_tag="SI621_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEI4065710.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15780..16007
                     /gene="rpsR"
                     /locus_tag="SI621_003023"
     CDS             15780..16007
                     /gene="rpsR"
                     /locus_tag="SI621_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEI4065711.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            16049..16498
                     /gene="rplI"
                     /locus_tag="SI621_003024"
     CDS             16049..16498
                     /gene="rplI"
                     /locus_tag="SI621_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEI4065712.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16569..17363)
                     /locus_tag="SI621_003025"
     CDS             complement(16569..17363)
                     /locus_tag="SI621_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HEI4065713.1"
                     /translation="MTLPTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRHTVEIHKLDLSDWQAFNKLATRCNTYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQGRDIRCDHMHLTPANHPELWTQENGEWIYLRN"
BASE COUNT         4165 a         4122 c         4628 g         4465 t
ORIGIN      
        1 tagctggtaa gataattacg tactgcaaat attcatttta ttaatattta aacttaattt
       61 atatggaata atatatgaca tggaatccgt tggcgctggc gacggcgctg caaactgtac
      121 ctgaacaaaa tattgatgta acaaatggcg agaacgcatt aattattaaa atgaatgatt
      181 atggcgattt gcaaatcaat attctattta cttcccgcca aatgattatc gaaaccttta
      241 tttgtccggt aagtagtatc agcaatccag atgaatttaa taccttctta ttaagaaatc
      301 agaagatgat gccgttgtca tcggtaggga tctacagcgt gcaacatgaa gagtattaca
      361 ttgttttcgg tgcgttatcg cttaaatctt ctcttgaaga tatcctgctt gagataacct
      421 cgctggtaga taacgcattg gatctggctg aaattacaga agaatattca cactaattac
      481 aaggacgggt atttatggga attttaaaaa gtttatttac gctggggaaa tcgtttatct
      541 ctcaggcgga agaatccatt gaagaaaccc agggcgtgcg catgctggaa cagcatattc
      601 gtgacgctaa agctgaactc gataaagccg gaaaatctcg cgttgatctg ctggcgcggg
      661 tgaaattaag tcacgataag ctgaaagatt tacgtgagcg caaagccagt ctggaagccc
      721 gtgcgctgga agcgttaagc aagaacgtta atccgtcgtt gattaacgaa gttgctgaag
      781 aaatcgcgcg ccttgagaat ctcattaccg ctgaagagca agtgttgtcg aatctggaag
      841 tctctcgtga tggcgtggaa aaagcagtta cagcgacagc gcagcgtatc gctcagtttg
      901 agcagcaaat ggaagtcgtt aaagctactg aagccatgca gcgtgcacaa caggctgtaa
      961 cgacctctac cgttggcgca tcttccagcg tttcgacagc ggcagaatcc ttaaaacgcc
     1021 tgcaaacgcg tcaggccgaa cgtcaggctc gcctggatgc tgctgcacag ttggaaaaag
     1081 tcgcagacgg tcgcgatctt gatgaaaagc tggcggaagc cggaataggc ggtagcaata
     1141 aaagtagcgc ccaggatgta ttagtaagac tgcaacgcca acagggcgag taattttttt
     1201 gccagcctca ctgcaaggct gtcaactatt ttaaggataa aatatgtctg gttttttcca
     1261 gcgtctgttt ggcaaggata ataagcctgc tatcgctcgc ggtccgctgg gacttcatct
     1321 caatagtggc tttacgctcg acacgttagc gtttcgtttg ctggaagatg aattgctgat
     1381 tgcgctgccg ggtgaggaat ttacggtagc cgctgtcagt cacatcgatc tgggcggcgg
     1441 tagccagatt ttccgctact acacttcagg cgatgagttt ctgcaaatca ataccaccgg
     1501 cggcgaaaat attgatgaca ttgatgatat caagctcttt gtctatgaag agagctacgg
     1561 tatcagtaaa gagagtcact ggcgcgagac gatcaatgcc aaagcgatgg gggcaatgac
     1621 cttaaactgg caggaaaaac gctggctgcg attttttaac agcgaagaac cgggaaatat
     1681 cgaacccgtc tacatgctgg aaaaagtaga aaatcaaaac catgccaaat gggaagtaca
     1741 taattttacc atgggctacc aacggcaagt aaccgaagat acttacgaat atttgctgct
     1801 aaatggtgag gaatctttta acgatctcgg cgagccagag tggttatttt cgcgcgcgtt
     1861 gggtgtcgat atcccgctga catcacttca tattattggt taattacaag gacgtttaca
     1921 atgcatatac tggattctct tcttgccttt agtgcctatt tttttattgg cgtggctatg
     1981 gtgattattt ttctgtttat ctattctaaa attacaccgc acaacgaatg gcagttaatc
     2041 aaaaacaata ataccgcggc gtcactggcg ttcagcggta cattgttggg ttacgtgatc
     2101 cccttatcca gtgcggcaat caatgcggtg agtattccag actatttcgc ctggggcggg
     2161 attgctctgg tgattcaatt actcgttttt gctggcgtca ggctttatat gcccgcatta
     2221 agcgaaaaaa ttattaatca caataccgca gcaggaatgt ttatgggaac cgccgcactg
     2281 gctggttgta tttttaacgc agcttgtatg acatggtaat ggacgatcat ggccagaaaa
     2341 cgcaaatcaa gaaataacag taaaattggt cacggagcga tcagtcgcat tggcagaccg
     2401 aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac gttggcgcta
     2461 atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtggcga tgtcgataac
     2521 gacggcgacg gaacgtttta ctcgactgtg caggattgta ttgatgacgg taataattca
     2581 gatatctgtg ctcatggctg gaacaacgcc aaagcggcgt tttatgtcga tgttccgaag
     2641 aatatgactc agcagaattg tcagtctaag tatgaaaatt gttactacga caacgttgaa
     2701 cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg taaagatcgc
     2761 gatgagcagt ttgtgtataa cagcggtggt tcctcttttg cttcgcgccc cgtctggcgc
     2821 aacacttctg gtgattactc ctggcgctcc ggttctggca aaaaagagtc ttactcttcg
     2881 ggcggcttta ccaccaaaaa agcgtcaacc gtttctcgcg gcggctatgg tcgttcttcc
     2941 agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct gtgcggcggg
     3001 atctggacca gatcgccgct gacaacggtt tcgactttca tatcatcgac aatgaaatct
     3061 attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag cagatcgaaa
     3121 aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg gttaaagacg
     3181 aagagatcct gacgcaactg gcgatccctc cgttgtactg ggatgtgatc gctgaaagct
     3241 ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt tgcctggtgt ggcaatgcgc
     3301 cggtgaagct gctggagtac aacgccgata cgccaacctc gttgtatgaa tcggcttatt
     3361 ttcagtggct gtggctggag gatgcccggc gcagcggcgt tattccgcgt gataccgatc
     3421 agtacaatgc cattcaggaa cgcctgattt cgcgctttag cgagctttat agtcgggagc
     3481 cgttttattt ttgctgttgt caggacactg acgaagacag gagtaccgtg ctgtacttgc
     3541 aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa gatctcggtt
     3601 tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt gcatttaagc
     3661 tgtacccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgcgc aagcgccgcg
     3721 agcaatgggt ggagccgtta tggaaaagta tcttgagtaa caaagggcta atgccgctgc
     3781 tttggcgctt ctttcctggt catcctaatc ttctggcgtc ctggtttgag ggcgaaaaac
     3841 tgcagatcgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc gagggcggta
     3901 acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgacggt gattacgccg
     3961 atgaaccgat gatctatcag gcgtttcaac ctctaccgcg atttggcgat agttacacgc
     4021 tgattggtag ctggattgtc gatgatgaag cgtgcggtat gggtatccgc gaagataaca
     4081 cgctgatcac taaagacact tcgcgttttg ttccgcacta cattgcgggt taagaatgtt
     4141 ttagcaatct ctttctgtcg ggaatccgtg gcagtgacca tactaatggt gactgccatt
     4201 gatggaggga gacacagtgc actggcaaac tcacactgtt tttaatcaac ctataccctt
     4261 aaataacagc aatttatact tatctgatgg cgcgctctgc gaagcggtaa cacgcgaagg
     4321 cgctggctgg gatagcgatt ttctcgccag tattggtcag caactcggaa cggctgaatc
     4381 ccttgaactg gggcgactgg cgaatgtaaa cccacctgaa ctattgcgct acgatgccca
     4441 ggggcggcgt ctggacgatg tccgtttcca tcccgcatgg cacctgctga tgcaggcgtt
     4501 atgtaccaat cgggtacaca acctggcctg ggaagaagac gctcgctccg gcgcatttgt
     4561 ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt tatgtccgat
     4621 aaccatgacc tttgccgcca cgccattgct gttacagatg ttacccacgc cgtttcagga
     4681 ctggaccacg ccgctgttga gcgatcgcta cgattctcac ttattgccag gtgggcaaaa
     4741 acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggcggttccg atgtgatgag
     4801 caacaccacc cgcgcagagc gtctggaaga tggctcttat cggctggtgg ggcataaatg
     4861 gtttttctca gtgccacaaa gcgatgcgca tctggtgctg gcgcagaccg cgggcggtct
     4921 gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga ttcgcctcga
     4981 gcggctgaaa gacaagctgg gtaatcgttc caatgccagt tgcgaagtgg agtttcagga
     5041 tgccattggc tggttgttgg gtcaggaagg ggaagggatt cgtctgatcc tgaaaatggg
     5101 tgggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc gtgcgttctc
     5161 gctggcgata tatcacgctc atcaacgcca tgtatttggt aatccgttaa tagaacagcc
     5221 ccttatgcgc catgtcttaa gtcgcatggc gcttcagctt gaagggcaaa cggcgttgct
     5281 gtttcgtctt gcgcgagcgt gggatcggcg tgccgatgcc aaagaagccc tgtgggcgcg
     5341 cttatttacg cctgcggcga aatttgtgat ctgcaaacgt ggcattccgt ttgtggctga
     5401 ggcgatggag gtgctgggcg gtattggcta ttgcgaagag agcgagctgc cgcggcttta
     5461 ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt gcctggatgt
     5521 gctgcgcgtt cttaataagc aagcgggagt atacgacttg ctgtcggaag cgtttatgga
     5581 agtgaaaggg caagatcgtt attttgatcg cgcggttcgt cgtttacagc agcagttgcg
     5641 taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc tgggctgcgg
     5701 tgcgcaaatg ttgaaatatg cttctccgcc aatggcacag gcgtggtgtc aggtgatgtt
     5761 aaatacgcgc ggtggcgtgc ggttgtcaga gcagatccag aatgatttat tgctgcgggc
     5821 gacgggggga gtgtgtttgt aagcgtatac gacggatgcg acgctggttt cgattaacta
     5881 aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc ggcattgttc
     5941 attcgcttca gatgacactc acgcatacag tatcgcctgt acacgccagt tatcaggctg
     6001 ttggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat cggcctttaa
     6061 cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg agatctgacc
     6121 tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact gtgccgtggc
     6181 gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt tcatcgtcag
     6241 ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata aacgcccagg
     6301 cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag caatgtaaag
     6361 ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa taccactgtc
     6421 ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt gcagcgacag
     6481 ctttcgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc gtgcttacgc
     6541 tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga tggtccgtta
     6601 ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac attaacacca
     6661 ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc atagtgtagt
     6721 gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac gcgattttcg
     6781 aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat accgccagca
     6841 gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga cagatgattg
     6901 aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct gtcgggcaaa
     6961 aagatacccc gttaccgtgc gcgatttttt atcacggctt tacttcgtcc agtctggtgt
     7021 atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg ccggatgcgc
     7081 ccgatcacgg tagccgtttt aggggcgacg cagcgcggcg gttaaatcaa ttttggcaaa
     7141 ttttgctaca aagtatgcag gaattcacta cgttacgtgc ggcaatagcc gaagaaaact
     7201 ggctgcttgc tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg acggcactgg
     7261 gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga tcgggctatt
     7321 ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca gcacagcaga
     7381 atgaattcaa taacattgtc gcgccgctgg cagagtggga agcgacaaac cacctggaac
     7441 aacttggtga cagacctcta ctgctgtggc atggccttga cgatgacgtc gtgcctgccg
     7501 acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa ctgctaacct
     7561 gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat gctgccgtga
     7621 catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct tccagttgtt
     7681 gcaggatttc cgggttagca tttttgccgg tgatcagcat atcgatttga tcggcacggc
     7741 taaaaagcat tcccgcgcgt tcgccgatct tactgctatc aaccagtacc accagtttcc
     7801 ctaccacgct tagcatcttc tgctctgcca ttgccgtcag catatcggtt ttatacaacc
     7861 cttctgctgt cagccctttg ccgctggtaa acatccagtg cccggcatag agactgtttt
     7921 cgctgccttg cgggcttaaa gtgatggact gacttttgtt gtattgtccg cccataatga
     7981 tcacgctgtc atgctcctga tcgatcaggt aatttgccag cggtagataa ttagtgatga
     8041 tttgcacagg cttgccacac atttcccgcc caagcagaaa cgcggtggag ccgcagttga
     8101 tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata cgtacttttt
     8161 catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgtgggcgc tgctgggtaa
     8221 tagcttctgc gccattgcgc acttttttca gtttgccgct ttcgtcaagt ttattgatat
     8281 cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca acggtcacaa
     8341 agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca gtcatgagct
     8401 attccgataa aatttgattt ttttagatga tatttgaaat agccaggaaa tactacgccg
     8461 gatagcgcga actactccac gctatccggt ataaacgaca gattacagga atgacttgaa
     8521 cggcagatcc ggttcaatag tgaagcaatc atcgaaacca cgcggatagt ggtactcgaa
     8581 gttgtcttta tccaacggcc aggtaaattt gccgcccacc tgccagataa acggcttgaa
     8641 gccatacttc agacgatctt ttttcatctc ccacagcacg cggatttctt gcggatcggc
     8701 ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat tcagggcttc
     8761 acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg ggttctcacc
     8821 atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat aatagttaga
     8881 gtagtgggaa tcgccactgt gatacaggga accgccaggg gttttgaaca ggtagtttac
     8941 tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg caggcagggt
     9001 gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt tcacttttac
     9061 aacatcgcca ggtttgacca cgatgcaacg ctctttcggt acgccccagc caatccacaa
     9121 atcaacgcag gttttcggtc caatgaacgg tacgtcatca gcacagttct gcatcacggc
     9181 agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta ccgcgtcgat
     9241 ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgcaggtttg gctgcagttt
     9301 tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt taccgtgact
     9361 ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt ctgatttcag
     9421 ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga cctgctcttg
     9481 ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga tccaggattc
     9541 acgggtgata cttttcactt tactcatcgc catttacctt catgatagtt caattcgaat
     9601 caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc aatgaccatt
     9661 ttttgacttt ttgccaggga agttgttgtt gattttgagt atggaaagat ttaatggaat
     9721 gtgtaattca attaactgaa tgaatttaaa tgaataattg tttcgttgtg tgaatcccac
     9781 tctatccatg tggaattatt tgcgggtcgc gtcacattta atcataaata atcttgttgt
     9841 gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgtggaa tacccggtta
     9901 cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt gttttttaac
     9961 caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg ctacatccta
    10021 ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat tggtttcatg
    10081 ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt ggcgaaaatg
    10141 tccgaagtct acggcattaa cggcgcaatt tccgatactt acgcatcgat gatggcaacc
    10201 atcgaccgca tgggcgatgc ctatagctgg gtaggttacg cggtgttgtt agcgctggca
    10261 ctgaacatct gttacgtgct gctgcgtcgc attaccggta ttcgtaccat catgctgact
    10321 ggtcatatca tgttccagca ggcggggctg attgccgtta cgttgtttat tttcggctac
    10381 tccatgtgga cgaccattat ctgcacggcg attctggttt cgctctactg gggcattacc
    10441 tccaacatga tgtacaagcc gactcaggaa gtgacggacg gctgcggttt ctccatcggt
    10501 caccagcaac agtttgcatc atggattgcc tataaggttg cgccgttcct cggcaaaaaa
    10561 gaggaaagcg ttgaagacct caaactgcca ggctggctga atattttcca cgacaacatt
    10621 gtctccacgg cgattgtcat gaccatcttc tttggtgcca ttctgctctc cttcggtatc
    10681 gacaccgtgc aggcgatggc aggcaaagtg aactggacgg tctacatcct gcaaactggt
    10741 ttctccttcg cggtggcgat cttcatcatc actcaaggcg tgcgcatgtt tgtggcggaa
    10801 ctctctgaag cgtttaacgg tatctcccag cgcctgatcc ctggtgcggt tctggcgatt
    10861 gactgtgcgg ctatctatag cttcgcgccg aacgccgtgg tctggggctt tatgtggggc
    10921 accatcggtc agctgattgc agttggcatc ctggtcgcct gcggttcctc gatcctgatt
    10981 attcccggct ttatcccgat gttcttctcc aacgccacca tcggcgtgtt cgctaaccac
    11041 ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga tggggatgat cgaaatcttt
    11101 ggttgcgtct gggcggtgaa acttaccggt atgagtgcct ggatgggtat ggcggactgg
    11161 tcgattctgg caccaccgat gatgcagggc ttcttctcca tcggtatcgc ctttatggcc
    11221 gtcatcattg taattgcact ggcttatatg ttcttcgctg gccgcgcgct gcgcgcagaa
    11281 gaagatgcag aaaaacaact ggcagaacag tctgcttaat aaggagtttt gattatgacc
    11341 gtacgtattc tggctgtgtg tggcaacgga caaggcagtt ccatgatcat gaagatgaaa
    11401 gtggaccagt ttttaaccca atcaaacatt gaccacacgg taaacagctg cgcggttggc
    11461 gaatacaaaa gcgagttgag cggcgcggat atcatcatcg cttctacgca cattgcgggc
    11521 gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc gcaacatgct ctctcctgct
    11581 gactttggcc cgaaactgct ggaagtgatc aaagcgcatt tcccgcagga tgtgaagtaa
    11641 ggacgcgcca tgaaattacg tgattcgctg gcggaaaata aatccatccg cctgcaggct
    11701 gaagctgaga catggcagga cgcagtaaaa atcggcgttg acctgctggt agcggcggat
    11761 gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg ttgaacagca cggtccgtac
    11821 tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc cggaagaggg cgttaagaaa
    11881 accggttttg cgctagtgac gctgaaaaag ccgctggagt tcaaccacga agataacgat
    11941 ccggtggata tcctcatcac catggcggcg gtcgatgcca acactcacca ggaagtgggc
    12001 atcatgcaga tcgtcaatct gtttgaagat gaagagaatt tcgaccgttt acgtgcctgc
    12061 cgtaccgagc aggaagtact ggatttaatt gaccgcacca acgcggcagc ttaagaagga
    12121 attgaacatg tcattaccga tgttgcaggt cgcgctggac aaccagacta tggatagcgc
    12181 ctatgaaacc actcgcctga ttgccgaaga agtcgacatt atcgaagtgg gtaccattct
    12241 gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa gcgctttacc cgcacaaaat
    12301 cgtgctggca gacgccaaaa ttgccgatgc aggcaaaatc ctttcgcgta tgtgcttcga
    12361 agccaacgct gactgggtga ccgtgatttg ctgtgcggat atcaacaccg ccaaaggcgc
    12421 gctggacgtg gcaaaagagt ttaacggcga cgtgcagatc gaactgactg gctactggac
    12481 ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag caggtggttt atcaccgcag
    12541 ccgtgacgcg caggccgcag gcgtggcgtg gggcgaagct gacattaccg cgatcaaacg
    12601 tctgtccgat atgggcttta aagtgaccgt caccggtggc ctggcgctgg aagatctgcc
    12661 gctgttcaaa ggcattccga ttcatgtgtt tatcgctggt cgcagtatcc gtgatgccgc
    12721 ttctccggtt gaagctgcgc gtcagttcaa acgttccatt gccgaactgt ggggctaagg
    12781 agcggatatg ttgtccaaac aaatcccgct tggcatctat gaaaaagcgc tccccgccgg
    12841 ggagtgctgg ctggaacggc tgcaactggc aaaaacgtta ggcttcgatt ttgtcgagat
    12901 gtcggtagat gaaacagacg atcgcctgtc gcgcctcgac tggagccgcg agcagcgtct
    12961 ggcgctggtg aatgcgattg ttgaaaccgg cgtgcgtgta ccgtctatgt gcctttctgc
    13021 tcatcgtcgt ttcccgctgg gcagcgaaga tgacgcggta cgggcgcagg ggctggagat
    13081 tatgcgtaaa gctatccagt tcgcccagga cgttggcatt cgcgtaatcc aactggcggg
    13141 ctatgacgtt tactatcagg aagccaataa cgaaacgcgt cgtcgtttcc gtgacggcct
    13201 gaaagagagc gttgagatgg caagccgcgc gcaggtgacg ctggcgatgg agatcatgga
    13261 ttacccgttg atgaactcca tcagtaaggc gttgggttac gcgcactatc tcaacaaccc
    13321 gtggttccag ctctacccgg atatcggcaa cctgtcggcg tgggacaacg acgtgcagat
    13381 ggaattgcag gccggaattg ggcatatcgt cgcggtgcat gtgaaagaca ccaaaccagg
    13441 cgtcttcaaa aacgtgccgt ttggcgaagg tgtggtggat ttcgaacgtt gcttcgaaac
    13501 gctcaaacag agtggctatt gcgggccgta cctgattgag atgtggagcg aaacggcgga
    13561 agacccggcg gcagaagtgg cgaaagcgcg tgattgggtg aaagcgcgca tggcgaaagc
    13621 gggcatggtg gaggcggcat aatgcaaaag ctaaaacagc aggtatttga agccaacatg
    13681 gatctgccgc gctacgggct ggtgaccttt acctggggca acgtcagcgc cattgaccgc
    13741 gaacgcgggc tggtggtgat caagcccagc ggcgttgcct acgaaaccat gaaagcggcc
    13801 gatatggtgg tggttgatat gagcggcaag gtggtggaag gggaatatcg cccgtcttcc
    13861 gacaccgcga cgcatctcga actctaccgt cgttatccgt cgcttggcgg cattgtccat
    13921 acccactcca ctcatgctac cgcatgggcg caggcggggc tggcgatccc ggcgttaggc
    13981 actacgcacg ccgactactt ctttggtgat atcccgtgta cgcgcggatt aagcgaagaa
    14041 gaggtgcagg gtgagtacga actgaacacc ggcaaagtga ttatcgaaac gctgggcaac
    14101 gccgagcctc tgcatacacc ggggattgtg gtgtaccagc acgggccgtt cgcctggggg
    14161 aaagatgctc acgatgcggt gcataacgcg gtggtgatgg aagaagtggc aaaaatggcg
    14221 tggattgccc gcggcattaa cccacaattg aatcacatcg acagctacct catgaataaa
    14281 cacttcatgc gtaagcacgg tcccaacgct tattacgggc agaagtaaaa cacgcgctgc
    14341 ggaaatttcc ttcttcggga gataactggt ctaattccgc agccgttttt caaaaaaagc
    14401 cccctgcgaa gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag
    14461 cgataaatat ccgcgctggc gtgcatattg ccgttacttc ccggttcccg tatcaggatc
    14521 ggatggtagt acgttgcgcc ctgcgcatct gtctcttcat ttaatgcctg acgtgcttca
    14581 ctttcagtgg cgaagttatg gttgatataa atcacgccta agctttgcac atcgtccata
    14641 tttctggcct ggtggttatt aatttcaatg gctgcccatg tatttgcact tagcaaaagc
    14701 acagccagaa gggctaaaac acgactgaac atagatacct cctcaacggc tgactttgtg
    14761 tgctctcctc tgtgatgatc ttctgattta attttaatca atgataaaga agttgatggt
    14821 gaccatttct gatgcagttg ttcaaaaaaa caccatgatg aagtgtgatg aacttcaaat
    14881 cagcgtgtta gtacgatttg gtgaaagatg agatttattt gtcggctggc cttgagttta
    14941 gcggggtatg tcagtactat aacgcgtcat ttttcagccg acctttaaca cgttccttgc
    15001 ctccccggga ttcggctgac ccagacagga ggctgaataa tccgtaagga gcaattcgat
    15061 gcgtcattac gaaatcgttt ttatggtcca tcctgaccag agcgaacagg ttccgggcat
    15121 gatcgagcgc tacactgctg ccatcactgg tgcagaaggc aagatccacc gtctggaaga
    15181 ctggggccgc cgtcagctgg cttacccgat caacaaactg cacaaagcac actacgttct
    15241 gatgaacgtt gaagctccgc aggaagtgat cgatgagctg gaaactacct tccgcttcaa
    15301 cgatgccgtt atccgcagca tggttatgcg taccaagcac gctgttaccg aagcatctcc
    15361 gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc
    15421 tgatgatgct gaagctgggg attctgaaga gtaatttctg atgaccaacc gtctggtgtt
    15481 gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg
    15541 ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc ggttttcacc ggcaggcgtg
    15601 gtgccaaatg cccgttattg ttagcggaca cgaaaaccag gccattactc acagtataac
    15661 ggtcggcagt cgcataaccg ttcaggggtt catttcatgc cacaaggcaa agaacggact
    15721 gagcaaaatg gttttgcatg ccgagcagat tgaattgata gattctggag actagccata
    15781 tggcacgtta tttccgtcgt cgcaagttct gccgtttcac cgcggaaggc gttcaagaga
    15841 tcgactataa agatatcgct acgctgaaaa actacatcac cgaaagcggt aagattgtcc
    15901 caagccgtat caccggtacc cgtgcaaaat accagcgtca gctggctcgc gctatcaaac
    15961 gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc
    16021 attaatacga ctttgagagg ataaggtaat gcaagttatt ctgcttgata aagtagcaaa
    16081 cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc tatgctcgta acttcctggt
    16141 accgcagggt aaagctgttc cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg
    16201 cgctgaactg gaagctaaac tggctgaagt tctggcagct gctaatgctc gcgctgagaa
    16261 aatcaatgca ctggaaactg ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt
    16321 cggttccatc ggtactcgcg acatcgctga cgctgtaact gcagctggcg ttgaagtggc
    16381 taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc actggcgaac acgaagtgag
    16441 cttccaggtt cacagcgaag tattcgcgaa agtgatcgta aacgtagtag ctgaataatt
    16501 cgttattcaa cgagacgtaa aaacgccgac tactggtcgg cgttttgctt tctatttttc
    16561 gtcaggtatt agtttcgcaa gtagatccat tcaccattct cttgtgtcca taattcagga
    16621 tggtttgctg gcgttagatg catatgatcg caccttatgt ctcggccctg cggcactcgc
    16681 tcgaaaccca aacccgagta gattgctgct gaacttgata aggaacgcag accaattttc
    16741 aaatcatggg gtaaatcaga atacgcgttt agcaatgcag caatgatgaa tccgagggca
    16801 ttagaaacgc ctgtaacagt gcgaagttgg tcgatataaa gatcgattgt tggtagttca
    16861 gatgaccagc aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg
    16921 gtatagtctg gagtaccata acctggaccc aaaacaacct cgtgtattgt gtatttactg
    16981 ttgggttcga aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca
    17041 cccgcgtaag ctttattgta tttctgggcg tgaatattat ctaatgtagc ttcatggttc
    17101 catccaaaag aattatcacc attcattgtt attccatcgt aagtattaca cctcgtcgct
    17161 aacttattga atgcttgcca gtctgagaga tctagtttgt ggatctcaac agtatgtctg
    17221 ccatcgcaat tatggtctct tccacgattt gaaagtaatg tagcgcgtgc gtattgatga
    17281 aattttaatt ttactggttt gtcggtggac gaaaaacggg gtgaatatgc aggaaatgaa
    17341 taaatagttg ttggtaatgt cattttattg ttgcctgttt
//
LOCUS       DARNVM010000100        17360 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.100, whole genome shotgun sequence.
ACCESSION   DARNVM010000100 DARNVM010000000
VERSION     DARNVM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.100"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(355..1551)
                     /gene="hemY"
                     /locus_tag="SI621_003026"
     CDS             complement(355..1551)
                     /gene="hemY"
                     /locus_tag="SI621_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HEI4065714.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADDGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1554..2801)
                     /gene="hemX"
                     /locus_tag="SI621_003027"
     CDS             complement(1554..2801)
                     /gene="hemX"
                     /locus_tag="SI621_003027"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEI4065715.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQADTPAAAPQGE"
     gene            complement(2823..3563)
                     /gene="hemD"
                     /locus_tag="SI621_003028"
     CDS             complement(2823..3563)
                     /gene="hemD"
                     /locus_tag="SI621_003028"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEI4065716.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKIADNADNDALLRALQ"
     gene            complement(3560..4501)
                     /gene="hemC"
                     /locus_tag="SI621_003029"
     CDS             complement(3560..4501)
                     /gene="hemC"
                     /locus_tag="SI621_003029"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEI4065717.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4888..7434
                     /gene="cyaA"
                     /locus_tag="SI621_003030"
     CDS             4888..7434
                     /gene="cyaA"
                     /locus_tag="SI621_003030"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HEI4065718.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7474..7794)
                     /gene="cyaY"
                     /locus_tag="SI621_003031"
     CDS             complement(7474..7794)
                     /gene="cyaY"
                     /locus_tag="SI621_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEI4065719.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7917..8102)
                     /locus_tag="SI621_003032"
                     /pseudo
     CDS             complement(7917..8102)
                     /locus_tag="SI621_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8261..8464
                     /locus_tag="SI621_003033"
     CDS             8261..8464
                     /locus_tag="SI621_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4065720.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8501..9325
                     /gene="dapF"
                     /locus_tag="SI621_003034"
     CDS             8501..9325
                     /gene="dapF"
                     /locus_tag="SI621_003034"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEI4065721.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTNDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9322..10029
                     /locus_tag="SI621_003035"
     CDS             9322..10029
                     /locus_tag="SI621_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HEI4065722.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10026..10922
                     /gene="xerC"
                     /locus_tag="SI621_003036"
     CDS             10026..10922
                     /gene="xerC"
                     /locus_tag="SI621_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEI4065723.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQYDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10922..11638
                     /gene="yigB"
                     /locus_tag="SI621_003037"
     CDS             10922..11638
                     /gene="yigB"
                     /locus_tag="SI621_003037"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HEI4065724.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11722..13884
                     /gene="uvrD"
                     /locus_tag="SI621_003038"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11722..13884
                     /gene="uvrD"
                     /locus_tag="SI621_003038"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEI4065725.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13929..14831)
                     /locus_tag="SI621_003039"
     CDS             complement(13929..14831)
                     /locus_tag="SI621_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEI4065726.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(14914..15677)
                     /locus_tag="SI621_003040"
                     /pseudo
     CDS             complement(14914..15677)
                     /locus_tag="SI621_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            16047..16997
                     /gene="corA"
                     /locus_tag="SI621_003041"
     CDS             16047..16997
                     /gene="corA"
                     /locus_tag="SI621_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEI4065727.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17038..>17360)
                     /locus_tag="SI621_003042"
     CDS             complement(17038..>17360)
                     /locus_tag="SI621_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HEI4065728.1"
                     /translation="YLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYED
                     LYCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK
                     KDNF"
BASE COUNT         3980 a         4524 c         4612 g         4244 t
ORIGIN      
        1 tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata aaaagtgtta
       61 agggatgtta tttcccggtt ctctgtggca taataaacga gtagatgctc attccatctc
      121 ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt acggtgctta
      181 tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa cgttgagtga
      241 agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata agagcaggcg
      301 tttttttatg tatcagaaag gtcccggagg tgcttgcctc cgggtgagaa agaactactg
      361 tggcgggttg ttctgcaacg ttaacatcaa accgtcgcgg cgcatcgctg cagcttcttc
      421 tggtttgtgc agtctgtcca gcgcgtcggc gagccatgcg taatcgtagg cgtccggacg
      481 ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc cattcaccgt gcttcatcag
      541 tgactgaccc aatgtgctcc acaacagcgg gcgatcaccg acgtttttga tttgctggcg
      601 tagcactttt tccagctgtt ccgggttatt ggttttcaga cgcgggatcg gcagcagcag
      661 acgatcatca tactggcgtt tcaggccgtc gatgataatt tgctgagcag tatcatgatc
      721 gtcacattca ataagatgtt ccgccattgc cacctgtaac gccacctgat gacgcgtttt
      781 ccggctttgg tttttccacc agtttcgcaa accttcgcta ccgtcatcgg cgcgcgcctg
      841 atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt catcgccaac
      901 gtgggctttc gccattgatg ggatgatatc cagcagcgaa ctccatgcac ctgtgcggat
      961 atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt ccagcagctt
     1021 atccacgccg tggcgtgcag catggttttc attacgggcc agttgaagac gtacacgggt
     1081 gatttctacc ggaatggtgt cgttgccggc cagctccgct gcacgttcca gatgttggtt
     1141 ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca gccagtagat agttcaccac
     1201 cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct gataatcgcc
     1261 ttccgctagt ttcagcagcg cctgctcggt ctgcttacgg gcacggcgac gcttacgtcc
     1321 gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc cgccgcagta gccactcaat
     1381 ggcaaacagc actaccatcg ccagaatcaa aataatcgcc aggccagtga cgctggtttc
     1441 gatattgtag ttgtcggtct ggatcagcac gtaaccctga tgaccggcaa tcatcgggcc
     1501 aaccactatc cccgcaatca gcaaaacaaa gagcaataac acttttagca tggttattct
     1561 ccttgcggcg cggctgccgg agtatcagct tgcggtgcag gtgcaggtgc aggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct
     1681 tccgttgtcc ccgctgccgg ttgtgccagc aggttacgca cgcgagtctg catcagtttt
     1741 tccagcatcg cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt
     1801 aactggtcca cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagca
     1861 cgtacccagg tggagacgtt ctccagcgcc tggcgataag tctcttgctg gtgacgcggt
     1921 acagcttgtg ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga
     1981 tttggcgcta acagcggtac ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc
     2041 ataaaattct gccagctttt ttgtagattg atacgccatt cgctgatgga actggaaagc
     2101 tcttcaccgt cggaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta
     2161 tctacctgat ttgaaagctg attaagctta aggatgatgc cgtcataatc cacctgcgat
     2221 accgcagaaa ggctggcgat atcatcggta attgcccgac gaacggtaat cagactcggg
     2281 tcattcatat ccgccaggct ggcatctgca cttttcagca acgctgcagc ggtcgtgacg
     2341 tcttgatcgc tccacagctt ccgtcctgcg agtttcacca gaaaatcggc ctgagccagc
     2401 agccaggttt tagcatcgct gccggaaatg gtggcgacct tttgttggac ttcatccaac
     2461 tgttttgcca gcgtttcttg ctgacgattc gcctgttcaa gctgcgtagc ctgttgcttg
     2521 ataatgcctt ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg
     2581 ttagccaggg catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac
     2641 aaaccgacgc ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcggta
     2701 ttgttcttac tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg
     2761 gtctcttcaa ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag
     2821 agttattgta atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgat cttaatgtct
     2881 tgccagccca gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag
     2941 tgtagtaacc agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt
     3001 tcaccgctgg taacaacgac cgtcgtcacc tcgcgggatt gccagcgcat cgcttcttct
     3061 gcaccatcgt aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg
     3121 cgcgccgtca gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca
     3181 cgtttgcccg caatattttg taattcaggt aattgtagca agacttcgct gatttcccga
     3241 tcctgcgggt agagaatctt ctgtccgctt acagtatgta gcgccagtgc ggtggtgcgt
     3301 ccaatggcga aataatcagg tagtcggggc catttacgat cttgctgatg cagctgtgat
     3361 tgggcaaaag caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct
     3421 gcaagttgat cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa
     3481 tgccaggcca cctgccccag tgtgcgcaga cggctcacta actcttctcc agcgggagac
     3541 gggcgggtga ccaggatact catgccgggg cgtctccgtt atagacttca gcaaggatct
     3601 cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatctt
     3661 gcggcgcacc gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg
     3721 cacgcagcca gatttcgcca tcaataagct cggcgtagct accaattggt acctgacatc
     3781 cgccttcgag acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt
     3841 gattcagcgc ggcaagcagc tcgcgagtgc gtgtatcatc aaggcggcat tcaataccga
     3901 ccgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg caacgcggcg cgaatgcgtg
     3961 actccagacc taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt
     4021 tatccagttt gctcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg
     4081 ggcggcgttc agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg
     4141 ccggtaacgc atccagactg tcatagttat tggacacaaa ggcatcgcga ggatcttcac
     4201 gctcacaaat agtgaccagt cccagacctt gcgggaattc aaccggcaca tctttcattg
     4261 aatgtacggc gatatcggcg cgattttcga ggagcgcgac ttccagctct tttacaaata
     4321 agccttttcc gcctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca
     4381 tcggtaccag ttcaacgacc aggcccggat ggctcgccat caacttgtct ttgacatagt
     4441 gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca
     4501 tgcttgttac cgtcattatc atccgtggcc catcctaaca tccttgccag agcgatgtca
     4561 gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc
     4621 tgacaggcgt ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt
     4681 gtatgtagcg catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag
     4741 accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt
     4801 gacatacgaa atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat
     4861 gacgggtagc aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga
     4921 ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc
     4981 caacaggtct acagtctact gccgacattg ttgcactatc accatccgct aatgccgggt
     5041 taccttgatg gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc
     5101 cactacctga acgagcttga actgtatcgt ggaatgtcag tacaggatcc gccgaaaggt
     5161 gagcttccaa ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt
     5221 tcctctgacc tggatatctg ggtctgtcat caatcctggc ttgatagcga agagcgccag
     5281 ttactacaac gtaaatgcag cctgctggaa agctgggccg cctcgctggg cgtggaagtt
     5341 agcttcttcc tgattgatga aaatcgcttc cgtcataatg aaagcggcag cctggggggc
     5401 gaagattgtg gctccaccca gcatatactg ctgcttgacg aattttaccg taccgccgtg
     5461 cgtctcgctg gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agagcattac
     5521 gacgactatg tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat
     5581 ctcggtggct taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc
     5641 tacaagagta ttgattcccc atacaaagcg gtgctgaaaa cactgctgct ggaagcctat
     5701 tcctgggaat acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac
     5761 ggcgagattg tgtcgtttgg cctcgatcct tactgcatga tgctggagcg tgttactgaa
     5821 tacctgacgg cgattgaaga tttcactcgt ctggatttag tacgccgctg cttctattta
     5881 aaagtgtgcg aaaaactcag ccgtgaacgc gcctgcgtag gctggcgtcg tgaagtgcta
     5941 acccagttag tgaaagagtg ggaatgggac gatgctcgtc tggcaatgct cgataaccgc
     6001 gctaactgga agattgatca ggtacgtgag gcgcacaacg agttgctcga cgcgatgatg
     6061 cagagctacc gtaatctgat tcgctttgcg cgccgcaata accttagcgt ctccgccagt
     6121 ccgcaggata tcggcgtgct gacgcgtaag ctgtatgccg cgtttgaagc attgccgggt
     6181 aaagtgacgc tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt
     6241 atttatgtgc cgccgggccg ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca
     6301 aatattgagt cgatcatcag ccatcagccg ctggaatata accgttacct gaataaactg
     6361 gtggcgtggg catggtttaa cggcctgctg acctcgcgta cccgcttgta tattaaaggc
     6421 aacggcattg tcgatttgcc taagttgcag gagatggtcg ccgatgtgtc acaccatttc
     6481 ccgctgcgcc tgcctgcacc gacgccaaaa gcgctctaca gcccgtgtga aattcgccat
     6541 ctggcgatta tcgttaacct ggaatatgac ccgacagctg cgttccgcaa tcaggtggtg
     6601 cattttgatt tccgtaagct ggacgtcttc agctttggcg agaatcaaaa ttgcctggta
     6661 ggtagcgtgg acctgctgta ccgcaactcg tggaacgaag tgcgtacgtt gcacttcaac
     6721 ggcgagcagt cgatgatcga agccctgaaa accattctcg gtaaaatgca tcaggacgcc
     6781 gcaccgccag atagcgtgga agtcttctgc tacagccagc atctgcgcgg attaatccgt
     6841 actcgcgtgc agcaactggt ttctgagtgc attgaactgc gtctttccag cacccgccag
     6901 gaaaccgggc gcttcaaggc gctgcgcgtt tctggtcaaa cctggggctt attcttcgag
     6961 cgcctgaatg tatcggtaca gaaactagaa aatgccatcg agttttatgg cgctatttcg
     7021 cataacaaac tgcacggcct gtcagtgcag gttgaaacca atcacgtcaa attaccggcg
     7081 gtggtggacg gctttgccag cgaagggatc atccagttct tcttcgaaga aacgcaagac
     7141 gagaatggct ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac
     7201 tgcgaaggta gcaaagagga gctggtgcgt gacgtcagtc gcttctactc gtcatcgcac
     7261 gaccgtttta cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt
     7321 gtgaaggttg atggtcgtga acaggtgatt ccgttccgca ccaaatcgat tggcaacatg
     7381 ccgcctgcca atcaggatca cgatacgccg ctattacagc agtatttttc gtgatgaacg
     7441 tgccggaaag cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc
     7501 ctgctgcgtc gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctac gatcgcaaat
     7561 ccactcatcg cctttcagat caaaatggta gccgccttgt ttggttgcca gccatacctg
     7621 gtgcagcggc tcctggcggt tgataatgat tttgctgccg ttttcaaagg taatggtcag
     7681 tacaccgccg ttgatttcac agtcgatatc gctgtcgcca tcccagtcgt ccagacgctc
     7741 ttcaatggtc agccacagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt
     7801 atcctgtttt taagtgatgg cggcagtata gcggcatggg gtcagggctt caaagtttgc
     7861 tcgtcttctg cgttctggca tgaatcatcc gtcaacggaa taataatgtc tctatatagc
     7921 gaaagattgg tcttttcata agggcgcaaa tacaccacgc cttttccctg tttctggtaa
     7981 atattatgtt ccggctcatc aagccgacat ccccatgccg acgataaaaa caacgaacaa
     8041 gccaacaaac aaaccacatt gcgatagtgc ataaagccat cctggcgcga ggtgccgatc
     8101 acgaaactac cagcaaaaca taaatcgcca cgagtaagcg ttatactcgc agcatttcct
     8161 cacttttcag acttcataaa gagtcgctaa acgcttgctt ttacgccttc tcctgcgatg
     8221 atagaaagca gaaagcgatg aactttacag gcaatccata atgaaaaacg tgtttaaggc
     8281 actcactgta ttacttactc tcttcagcct gacgggctgc ggtctgaaag gtccgctcta
     8341 tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa ccggtagaga cgcaaacgca
     8401 atccacagtg ccggataaaa acgaccgcgc cactggcgat ggtccatccc aggtgaatta
     8461 ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg atgcagttct cgaaaatgca
     8521 tggccttggc aacgatttta tggtcgtcga cgcggtaacg cagaatgtct ttttttcacc
     8581 ggagctgatt cgtcgcctgg cggatcggca cctgggggta gggtttgacc aactgctggt
     8641 ggttgagcca ccgtatgatc ctgaactgga ttttcactat cgcattttca atgctgatgg
     8701 cagtgaagtg gcgcagtgcg gcaacggcgc gcgctgcttt gcccgttttg tgcgtctgaa
     8761 agggctgacc aataagcgcg atatccgcgt cagcaccgcc aacggacgga tggttctgac
     8821 cgtcaccaat gatgatctgg tccgcgtcaa tatgggcgaa cccaacttcg aaccttctgc
     8881 cgtgccattt cgcgctaaca aagcggaaaa gacctatatt atgcgcgccg ccgagcagac
     8941 aatcttatgc ggcgtggtgt cgatgggaaa tccgcactgc gtgattcagg tcgatgatgt
     9001 cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa agccacgagc gttttccgga
     9061 gcgcgccaat atcggtttta tgcaagtggt taagcgcgag catattcgtt tacgcgttta
     9121 tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc gcgtgtgcgg cggtggcggt
     9181 agggatccag caaggtttgc tggccgaaga agtacgcgtg gaactccccg gcggtcgtct
     9241 tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg actggcccgg cggtacatgt
     9301 ctacgacgga tttattcatc tatgaagcaa ccaggggaag aactgcagga aacactcacg
     9361 gagcttgatg accgggcggt tgtcgattat ctgattaaaa atcctgagtt ttttatccgc
     9421 aatgcgcgcg cagttgaagc gatacgtgtg ccgcatccgg tacgcggcac cgtttcgttg
     9481 gtcgaatggc acatggcccg cgcacgtaat catattcatg ttctggaaga gaacatggcg
     9541 ctgttgatgg aacaggctat cgccaacgaa ggcctgtttt atcgcttact ctacctgcag
     9601 cgtagtctca ccgccgccag cagtctcgac gatatgctga tgcgctttca ccgctgggcg
     9661 cgcgatctcg gcctggcagg tgcgagtctg cgcctgtttc cagatcgctg gcgcttaggt
     9721 gcgccgtcga accacactca tctggcatta agccgtcagt ctttcgaacc gctgcgtatt
     9781 cagcgtctgg ggcaggaaca gcactatctg gggccgctta acggaccgga gcttctggtg
     9841 gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt cgatgctggg aagcgatgct
     9901 gatttgggtg ttgtgctgtt taccagtcgc gatgccagtc actatcaaca agggcaggga
     9961 acgcagttac ttcatgaaat tgcgctgatg ttgccggagc ttctggagcg ttggattgaa
    10021 cgcgtatgac cgatttacac accgatgtag aacgctacct acgttatctg agcgtggagc
    10081 gccagctaag cccgataacc ctgcttaact accagcgtca gcttgaggcg atcatcaatt
    10141 ttgccagcga aaacggcctg caaagctggc agcaatatga tgtgacgatg gtgcgcaatt
    10201 tcgctgtacg cagtcgccgt aaagggctgg gagctgcaag tttggcgtta cggctttctg
    10261 cgctacgtag cttttttgac tggctggtca gccagaacga gctcaaagct aacccggcga
    10321 aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa aaacatcgac gtcgacgata
    10381 tgaatcggct gctggatatt gatatcaacg atcccctcgc tgtgcgcgat cgggcaatgc
    10441 tggaagtgat gtacggcgcg ggtctgcgtc tttccgagct ggtggggctc gatatcaaac
    10501 acctcgacct ggagtctggt gaagtgtggg tgatgggaaa aggcagcaaa gagcgccgcc
    10561 tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg gcttgatttg cgcgacctgt
    10621 ttggaagcga agacgatgcg ctttttctgt cgaaactggg caagcgtatc tccgcgcgta
    10681 atgtgcagaa acgctttgcc gaatggggca taaaacaagg gctgaataat cacgttcacc
    10741 cgcataaatt acgtcactcg ttcgctacgc atatgctgga gtcgagcggc gatcttcgtg
    10801 gtgtgcagga gctgctgggt catgccaacc tctccaccac gcaaatctat actcatcttg
    10861 attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc acgcgccaaa cgggggaaat
    10921 aatgcgtttt taccggcctt tggggcgcat ctcggcgctc acctttgacc tggatgatac
    10981 cctttacgat aaccgtccgg tgattttgcg caccgagcga gaggcgctta cctttgtgca
    11041 aaattatcat ccggcgctgc gcagcttcca gaatgaagat ctgcaacgcc tgcgccaggc
    11101 ggtacgggaa gcggaacccg agatttatca cgacgtgacg cgctggcgtt ttcgttcgat
    11161 tgaacaagcc atgctcgacg ccgggctgag tgccgaagaa gccagtgcag gcgcacacgc
    11221 agcaatgatc aactttgcca aatggcgcag ccgaatcgat gttccgcagc aaacccacga
    11281 caccttaaaa cagctggcga agaaatggcc gctggtggcg atcaccaacg gtaacgccca
    11341 gccggagctg tttggtttag gggattattt tgagtttgtg ctgcgcgctg gcccgcacgg
    11401 gcgctcaaaa ccgttcagcg atatgtactt tctggctgcg gaaaaactca acgtgccgat
    11461 cggcgagatc ttacatgttg gggacgatct caccactgac gtgggtgggg caattcgcag
    11521 cggaatgcag gcttgttgga tcagaccgga aaatggcgat ctgatgcaaa cctgggacag
    11581 ccgtttactc ccgcatctgg aaatttcccg gttggcatct ctgacctcgc tgatataatc
    11641 agcaaatctg tatatatacc cagctttttg gcggagggcg ttgcgcttct ccgcccaacc
    11701 tatttttacg cggcggtgcc aatggacgtt tcttacctgc tcgacagcct taatgacaaa
    11761 cagcgcgaag cggtggccgc gccacgcagc aaccttctgg tgctggcggg cgcgggcagt
    11821 ggtaagacgc gcgtactggt gcaccgtatc gcctggctga tgagcgtgga aaactgctcg
    11881 ccatattcga ttatggcggt aacgtttacc aacaaagcgg cggcggagat gcgtcatcgt
    11941 atcgggcagc tgatgggcac cagccagggc ggcatgtggg tcggcacctt ccacgggctg
    12001 gcgcaccgtc tgctgcgtgc gcaccatatg gacgccaatc tgccgcagga ttttcagatc
    12061 ctcgacagcg aggaccagct acgcctgctc aaacgcctaa tcaaggcgat gaacctcgac
    12121 gagaagcagt ggccgccgcg ccaggcaatg tggtacatca acagccagaa agatgaaggc
    12181 ctgcgtccac atcatattca aagctacggt aatccggtgg agcagacctg gcagaaggtg
    12241 tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg tggacttcgc ggaactgctg
    12301 ctacgcgcgc acgagctgtg gcttaacaag ccgcatattc tgcaacacta ccgcgaacgt
    12361 tttactaaca tcctggtaga cgaattccag gataccaaca acattcagta cgcgtggatc
    12421 cgcctgctgg cgggcgacac cggcaaagtg atgatcgttg gcgatgacga ccagtcaatc
    12481 tacggctggc gtggggcgca ggtcgagaat attcagcgtt tcctcaatga tttccccggt
    12541 gctgaaacta tccgtctgga gcagaactac cgctctacca gcaatattct gagcgccgcg
    12601 aacgccctga ttgaaaacaa caacgggcgt ttgggtaaaa aactgtggac cgatggcgcg
    12661 gatggtgagc ctatttccct ctattgcgct tttaacgaac tcgatgaagc gcgttttgtg
    12721 gttaaccgta tcaaaacctg gcaggacaac ggcggggcgc ttgccgagtg cgccattctc
    12781 taccgcagca acgcccagtc gcgtgtgctg gaagaagcac tgttacaggc aagtatgccg
    12841 taccgtattt acggcgggat gcgattcttc gaacgccagg aaatcaaaga tgcgctctcg
    12901 tatctgcgcc tgattgccaa ccgtaatgac gacgcggcct ttgagcgcgt ggtgaataca
    12961 ccaacgcggg gtattggtga ccggacgctg gacgtggtac gtcagacatc gcgcgatcgc
    13021 cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg aaaaagccct cgccggacgt
    13081 gctgccagtg ccttacagcg gtttatggaa ctgatcgacg ccttagcgca ggaaactgcc
    13141 gatatgccgc tgcatgtaca gactgaccgg gtaattaaag actccggcct gcgcacaatg
    13201 tacgagcagg agaaaggcga aaaaggccag acgcgtatcg aaaacttaga ggaactggtg
    13261 acggcgacgc gccagttcag ctacaacgaa gaagacgaag atttaatgcc gctgcaggcg
    13321 ttcctctccc atgcggcact ggaagcgggc gaggggcagg cggatacctg gcaggatgcg
    13381 gtgcagttga tgacgctaca ctcggcgaaa ggtctggagt tcccgcaggt gtttatcgtc
    13441 ggtatggaag agggcatgtt cccaagccag atgtcgctgg atgaaggtgg gcgtctggaa
    13501 gaagaacgcc gtctggccta cgttggcgta acccgcgcga tgcagaaact gacgctgacc
    13561 tacgcggaaa cccgccgtct gtatggcaaa gaagtttacc atcgcccgtc gcgctttatc
    13621 ggcgagctgc cggaagagtg tgtggaagag gtgcgcctgc gcgccacggt aagccgcccg
    13681 gtcagccatc aacggatggg tacgccgatg gtcgagaacg acagcggcta caagctcggc
    13741 cagcgcgtac gccacgctaa gtttggtgaa ggcaccattg tcaatatgga aggcagcggt
    13801 gagcatagcc gtttgcaggt ggcgttccag ggccagggta ttaaatggct ggtggcggct
    13861 tatgcccggt tggagacggt gtgacgttgc gggatgcggt gcgccacacc gcattccgca
    13921 tcagcacctt acttagcgca cttcttcaca tcaatattcc agtcagcacc agacgcaacc
    13981 tgatgtttct gcgcaaaatt atccatattc agcgccacgg aaacattcaa taagctgttc
    14041 agatacacca tcggctggcc ttccggcaca tcgccgaagc ttgcgacata cggcattttc
    14101 cctgcgtatt tttgctggga accttcggaa atggtcacac acaacgtgtc gttgagtttg
    14161 attcctgcct gattcagcaa ctcgtcgctg atattgctcc agacgttgcc gtactgaata
    14221 tcgagaatcg ggatattgcc cttcacttca ccttttgttg cttttgcttt ctgataagaa
    14281 agttcgacaa cttttgcagg aagctctggc cccacctgtt cgaacgtaat cgctccggaa
    14341 gccagacgcg caccagtgta ggcatataca tcgcgaccat ggaaggtata tgacttttca
    14401 gaacctttca ggcggttagc tttttcgtcg atttcccgga cgctatcaat acccagagtt
    14461 tgtgccacca aagtgagcgt accgttatct ggtgaaacga agtattgacc gtttttggtt
    14521 ttcagcacta ccgatttacg gtcagtgcct acgcccggat cgaccacgct aacaaacacc
    14581 gaaccttgcg gccagtaact ggcagtctga tacaggcgat aagcgccttc ccagatgtta
    14641 tacggtggaa tttcgtgtgt taaatcaaag attttaagat tatggtcaac gccgaatgcg
    14701 acccctttca tcgccgagac ggcaccatct ttcaggctaa aatcagtttg taaaatcaaa
    14761 gcgttatccg cataggcgga tgatgatccc agacacgtta ataaaaataa cgttgcgagc
    14821 gtttttttca tttttgtttc ccttcattta tctgatgatt ttccagctgt gtagccagtc
    14881 ccagtttgtc tggcctcctc gagtgtacaa tgcttaacct ttccgctcga cggaaatcat
    14941 ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca tatgtgaaat
    15001 tgtgccatca agataaagca actgctcaac gttcagtttc gctttggcat aacaggcaaa
    15061 atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcgttccctt gtttattaat
    15121 cctacaccgt tacgaatttt acgcgaggca acattcgggt gaatgcgtgg attaattacg
    15181 ccgttttcta ataacattgg ccctgactgc accgcaaact gaatctcttt actggtttta
    15241 aaggcatcca gacgaacgat gccgacttta tctcccgcga cataaaacac gccgccagga
    15301 cgaataaaga aattcccttc acctgaagcg agatttaacg ccaccttctg ctgaccgttt
    15361 tcgatgtaca aaccgagcgg cgcatagctt tcatcataga tgccgccgtt catcgccatc
    15421 tgcacctgac cctgactatt catatccgcc agcagagcat gtaacgttcc ccacgcttcg
    15481 ccattggctt tttgccagta cattttcacc cgctctgttt gaggattaac ggtatacgcc
    15541 tgtacggtca gcgtcggatc tgagagtgcg caatcatcag cggcaacagc aaacaaggga
    15601 agtaaggtga gggcgagaaa aatccgtttg agattcaagg tgatcattcc tttaccaatg
    15661 agtagccgat gcgccattat aggtcctgga tgtgggattt ctttatcctg ttagcgacct
    15721 tgacgagtac caaaaagcgc gaagttcaac tattgttctg tggtgttctg ttgcgtgttg
    15781 acggcaaaat tttgctggcg taacatgcgc gcacgatcac tctaagagga cattcgcctt
    15841 ggacacaccc agtagatact ggctcactat cctgtcatcc aggatcaact cctaaggcta
    15901 tccctttttg ctgatagcct tagcggttgt cagcgacctc aatttttccc gtcgcgctga
    15961 gtcaggctgt ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg aactgtccga
    16021 tattttaagc attgggagtc ccggtcatgc tgagcgcatt tcaactggaa aataaccgac
    16081 tgacccggct ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg attgatcttg
    16141 tcgaaccgga cgacgacgag cgactgcgcg tacaatctga acttggtcag agcctggcaa
    16201 cccgcccgga actggaagac atcgaagcat cggcacgttt ctttgaagac gatgacggcc
    16261 tgcatattca ctccttcttc ttctttgaag atgcggaaga tcacgccggt aactccactg
    16321 tggcatttac catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa ctgcctgcat
    16381 tccgtctgta tcgtatgcgt gcccgtagcc agtcgatggt agacggtaat gcctacgagc
    16441 tgctgctgga tctgttcgaa accaaaatcg aacagttggc agatgaaatt gaaaatatct
    16501 acagcgacct ggagcagttg agccgggtaa ttatggaagg gcatcagggc gatgagtacg
    16561 acgaggcgct ctccacgctg gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc
    16621 tgatggatac ccagcgtgcg ctcaacttcc tggtgcgtaa ggcgcgttta ccgggcgggc
    16681 aactagagca ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg cataacgaat
    16741 ctctgttcca gaaggtgaac ttcctgatgc aggcggcgat gggctttatc aacatcgagc
    16801 agaaccgcat catcaaaatc ttctcggtgg tatccgtcgt gttcctgccg ccaacactgg
    16861 ttgcttccag ctacgggatg aactttgagt ttatgccaga actgaagtgg agtttcggct
    16921 acccaggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc
    16981 ggaagaactg gttgtaatat gagtgccgga taaagctatt ttttatccgg cttatttcta
    17041 aaaattgtct tttttaaagt catgcggatt gaaaactcgc tctattttat tttcttgtgt
    17101 taattgatac tcagtaaaat cgttaatacc aatatgagtg ttacgataag ggtattcatt
    17161 aataaccatt aagtaatttt tcacgatgca attatcattg ctcttaattg tcgggaaaca
    17221 gtagagatct tcatacggcc cttcgatata ttttttatag tcagtgaagc cattaccgat
    17281 atcaatgaca taccaggtat gccaactacc ttctggtagg ggatgttgat ttttaatttc
    17341 attctgatgc ttcagatacc
//
LOCUS       DARNVM010000101        17340 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.101, whole genome shotgun sequence.
ACCESSION   DARNVM010000101 DARNVM010000000
VERSION     DARNVM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.101"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..857
                     /locus_tag="SI621_003043"
     CDS             <1..857
                     /locus_tag="SI621_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEI4065729.1"
                     /translation="FVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHA
                     LQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
                     IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPE
                     GTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQ
                     IDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRA
                     ELSGIREE"
     gene            868..2400
                     /gene="citF"
                     /locus_tag="SI621_003044"
     CDS             868..2400
                     /gene="citF"
                     /locus_tag="SI621_003044"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEI4065730.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            2404..2955
                     /gene="citX"
                     /locus_tag="SI621_003045"
     CDS             2404..2955
                     /gene="citX"
                     /locus_tag="SI621_003045"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEI4065731.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHNHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKN
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            2930..3817
                     /gene="citG"
                     /locus_tag="SI621_003046"
     CDS             2930..3817
                     /gene="citG"
                     /locus_tag="SI621_003046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEI4065732.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTLADLDYLRQFDRECIERNLSP
                     GGSADLLIVTWFLASLTGR"
     gene            3843..6440
                     /gene="acnB"
                     /locus_tag="SI621_003047"
     CDS             3843..6440
                     /gene="acnB"
                     /locus_tag="SI621_003047"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEI4065733.1"
                     /translation="MLKEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDHRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DRVIFPSVV"
     gene            complement(6678..7288)
                     /locus_tag="SI621_003048"
                     /pseudo
     CDS             complement(6678..7288)
                     /locus_tag="SI621_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7283..7603)
                     /locus_tag="SI621_003049"
                     /pseudo
     CDS             complement(7283..7603)
                     /locus_tag="SI621_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(7692..8204)
                     /locus_tag="SI621_003050"
     CDS             complement(7692..8204)
                     /locus_tag="SI621_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065734.1"
                     /translation="MQITEALLSEPGDIWRFVQQAVDHWPRLLAVHFTLYSAEGNING
                     QQIHAFCTAFYRQVQEHITERNHTASPAPPVVLRWLLEQHGEATIRCLLLLSQASICH
                     LRVSATVDEECSQVVDLLQQTWSGINAGGQCRLESCFQVDRSGTLDQFVVLKTTVQSL
                     MLPVMATIAR"
     gene            8354..9521
                     /locus_tag="SI621_003051"
                     /pseudo
     CDS             8354..9521
                     /locus_tag="SI621_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273751.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
     gene            complement(9584..10408)
                     /gene="agaR"
                     /locus_tag="SI621_003052"
     CDS             complement(9584..10408)
                     /gene="agaR"
                     /locus_tag="SI621_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HEI4065735.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQRIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVLLDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTKRIDMIIVDEGIPADSLEGLRKAGIEVILVGELASSL"
     gene            10591..10995
                     /locus_tag="SI621_003053"
     CDS             10591..10995
                     /locus_tag="SI621_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEI4065736.1"
                     /translation="MTQYELKQNERLISQQSELERKVKHLTEMVRQYKAGKTNGIYAV
                     CFARFVLHGASDVPDEYVRRTIGPGVCKVNVATELKIAFSDAIKAWFAENQQSNDPCF
                     YMRVGMDTMKEVVRSKIAVCGSANRLRLPAEA"
     gene            11109..12680
                     /gene="malX"
                     /locus_tag="SI621_003054"
     CDS             11109..12680
                     /gene="malX"
                     /locus_tag="SI621_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HEI4065737.1"
                     /translation="MTQKKSFKSKLWEFLQSLGKTFMFPVSLLAFMGLLLGIGSSVTS
                     PSTITSFPFLGGEFTQLTFGFIAMVGGFAFTYLPLMFAMAIPMGLAKRNKAVAAFAGF
                     VGYMLMNMSINYYLTATHQLADPATMKQVGQSIVLGIQTLEMGVLGGIVVGVITYFLH
                     DRFQDTVLHDAFAFFSGIRFVPIITALTLSLVGLFIPMLWEYVALGIAGIGHIIQSTS
                     VFGPFLYGVGVLLLKPFGLHHILLAMVRFTPAGGIEMVNGHEVAGALNIFYAELKAGL
                     PFSPHVTAFLSQGFMPTFIFGLPAVAYAIYRTARPENRPVIKGLLLSGVLVSVVTGIS
                     EPIEFLFLFIAPALYAFHIVMSGLALMVMALLGVTIGNTDGGILDLLIFGVMQGMSTK
                     WYLLFPVGIAWFAIYFFVFRWYILKHNIKTPGREVDVQGAQQAVEANTRARGKSKYDH
                     ELILRALGGKENIESLDNCITRLRLVVKDMGLIDQQALKAAGALSVVVLDAHSVQVII
                     GPQVQSVKTGIEALI"
     gene            12692..13867
                     /locus_tag="SI621_003055"
     CDS             12692..13867
                     /locus_tag="SI621_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEI4065738.1"
                     /translation="MFDFDKIIERQSDKCRKWDHTFVCSRFGDVPESFIPLWIADMDF
                     TSPPAVIDGFRRIVEHGTFGYTWCFDEFYDAVIAFQRKRHQVEVEKSWITLTYGTVST
                     LHYTVQAFCKPGDSVMMNTPVYDPFAMAAQRQGVQVLANPLRVEENRYQLDFNLIEEQ
                     LKTHRPTLWFFCSPHNPSGRIWREEEIRQVSDLCQRYGTILVVDEVHAEHILDGKFVS
                     CLTSGCAAQDNLIVLTSPNKAFNLGGLKTSYSMIPDDSLRQRFRQQLEKNSITSPNLF
                     GVWGIILAYQHGLPWLDALNGYLQGNARYLADALQTHFPAWKMMNPESSYLAWIDVSA
                     DERSATQLTQHFARQAGVVIEDGSHYVQNGENYLRINFGTQRYWLEQSINRMLKNDK"
     gene            13881..15770
                     /locus_tag="SI621_003056"
     CDS             13881..15770
                     /locus_tag="SI621_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HEI4065739.1"
                     /translation="MKKVLTLSLLALCVSHGAAAANYALNNDNIALLFDDTNSTVVVK
                     DNKANHPLTPQELFFLTLPDESKIHTADFKIKHVEKQDNAIVIDFTHPDFNVTVKLNL
                     VKGKYANIGYTIAAVGQPRDVAKITFFPTQKQSQAPYVDGAINSSPIVADSFFILPDK
                     PIVNTYAYEATTNLNVELKTPIQPEAPVSFTTWFGTFPETSQLRRSVNQFINDVRPRP
                     YKPYLHYNSWMDIGFFTPYTEQDVLGRMDEWNKEFITGRGVALDAFLLDDGWDDLTGR
                     WLFGTAFRNGFSKVREKADSLHSSVGLWLSPWGGYNKPRDVRVSHAKEYGFETVDGKL
                     ALSGANYFKNFNERIIKLIKNEHITSFKLDGMGNASSHIKGSSFASDFDASIALLHNM
                     RSATPNLFINLTTGTDASPSWLFYADSIWRQGDDINLYGSGTPVQQWMTYRDAETYRS
                     IVRKGPLFPLNSLMYHGIVSAENAYYGLEKVQTDSDFADQVWSYFATGTQLQELYITP
                     SMLNKVKWDTLAKAAKWSKENASVLVDTHWIGGDPTALAVYGWASWSKDKAILGLRNP
                     SDKPQTYYLDLAKDFEIPTGDAAQFSLKAVYGSNKTVPVEYKNATVITLQPLETLVFE
                     AVPVN"
     gene            complement(15837..16091)
                     /locus_tag="SI621_003057"
                     /pseudo
     CDS             complement(15837..16091)
                     /locus_tag="SI621_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228991.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16098..16885)
                     /locus_tag="SI621_003058"
                     /pseudo
     CDS             complement(16098..16885)
                     /locus_tag="SI621_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16916..17266)
                     /gene="tnpB"
                     /locus_tag="SI621_003059"
     CDS             complement(16916..17266)
                     /gene="tnpB"
                     /locus_tag="SI621_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEI4065740.1"
                     /translation="MISRPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRCDTVKILWADSDGLCQFTKHLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNSLTML"
     gene            complement(17263..>17340)
                     /locus_tag="SI621_003060"
     CDS             complement(17263..>17340)
                     /locus_tag="SI621_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEI4065741.1"
                     /translation="GKMTLENPSPELLTVLIRELTGRGR"
BASE COUNT         3947 a         4781 c         4609 g         4003 t
ORIGIN      
        1 tgtttgtgcc aggtgccaat gccgcgatgg tcagcaactc attcatctac ccggctgatg
       61 ccctgatgtt tgacctcgaa gactccgtgg cattgcgtga aaaagacaca gcccgccgca
      121 tggtttacca cgcgctgcaa catccgctgt atcgcgatat tgaaaccatt gtgcgtgtaa
      181 acgcgctgga ttccgaatgg ggtgttaacg acctggaagc cgtcgttcgc ggtggtgcgg
      241 acgttgtgcg tctgccgaaa accgataccg ctcaggatgt tctggatatc gaaaaagaga
      301 tcctgcgcat cgaaaaagcc tgtggtcgtg aacccggcag caccggcctg ctggcagcga
      361 ttgaatcccc gctgggtatt acccgcgcag tggaaatcgc tcacgcttcc gagcgtttga
      421 tcggtatcgc cctcggtgca gaagactatg tgcgcaacct gcgtacagaa cgctccccgg
      481 aaggaactga actgctgttc gcacgctgtt ccattttgca ggccgcgcgc tctgcgggta
      541 ttcaggcgtt cgataccgtc tattccgacg ctaataacga agccggattc ctgcaagaag
      601 ccgcccacat caaacagctg ggctttgacg gcaaatcgct gatcaacccg cgtcagattg
      661 atctgctgca caacctctac gcaccgaccc agaaagaagt ggatcacgcc cgccgcgtcg
      721 tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt ggtttccctg aacggcaaga
      781 tggtggacgg tccggttatc gatcgcgccc gtctggtgct ctcccgcgca gaactttccg
      841 gcatccgcga agaataaggc aatcaaaatg acgcagaaaa ttgaacaatc tcaacgacaa
      901 gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc ttgccgcttt ccagaactcg
      961 ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca aactgtgcgc caacctggaa
     1021 gaagcgattc gtcgctctgg tttacaggac ggtatgacgg tttccttcca tcacgctttc
     1081 cgtggcggtg acctgaccgt caatatggtg atggacgtca tcgcgaagat gggctttaaa
     1141 aacctgaccc tggcctccag ctccctgagt gattgccatg cgccgctggt agaacacatt
     1201 cgccagggcg tggttacccg catttatacc tctggcctgc gcggtccact ggcggaagaa
     1261 atctcccgtg gtctgctggc ggaaccggtg caaatccact ctcacggcgg acgtgtgcat
     1321 ctggtacaga gcggcgaact gaatatcgat gtggctttcc tcggcgtccc gtcctgtgat
     1381 gaattcggta atgccaacgg ctactccggt aaagcctgct gcggctccct cggctatgca
     1441 atggttgatg ccgacaacgc aaaacaggtc gtgatgctta ccgaagaact gctgccttat
     1501 ccgcataatc cggcaagcat tgagcaagat caggttgatc tgatcgtcaa agttgatcgc
     1561 gttggcgatg ctgcaaaaat cggcgctggt gctacccgta tgaccactaa cccgcgcgaa
     1621 ctgcttattg cccgtagcgc tgcggatgtg attgttaact ctggctactt caaagaaggt
     1681 ttctccatgc agaccggcac cggcggcgct tcgctggcgg taacccgttt cctggaagac
     1741 aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg gcggtattac cgcaacgatg
     1801 gttgatctgc acgaaaaagg tttgatccgc aaactgctgg atgtgcagag ctttgacagc
     1861 catgctgcgc aatcgctggc ccgtaacccc aatcacatcg aaatcagcgc caaccagtac
     1921 gctaactggg ggtcgaaagg cgcatcggtt gatcgtctcg acgtggtggt actgagtgcg
     1981 ctggaaattg acacccagtt caacgttaac gtgctgaccg gctctgacgg cgtactgcgt
     2041 ggtgcttccg gtggtcactg cgataccgcg attgcctctg cgctttccat catcgtcgcg
     2101 ccgctggtac gcggtcgtat tccgacgctg gtggataacg tgctgacctg catcaccccg
     2161 ggctccagtg tcgatattct ggtcacagac cacggcatcg ctgttaaccc ggcacgtccg
     2221 gagctggcag aacgtctgca ggaagcgggg atcaaagtgg tttctattga gtggctgcgt
     2281 gaacgtgcgc gtctgctgac cggtgaacca cagccgattg aattcaccga ccgcgtcgtt
     2341 gccgttgtgc gttaccgcga tggctcggtg atcgatgttg tgcatcaggt gaaggaataa
     2401 gtcatgcacc tgcttcctga actcgccagc caccatgcgg tgtcaattcc cgagctgctc
     2461 gtcagccggg atgaaaggca agcacggcaa cacgcctggc tcaagcgcca tcctgttcca
     2521 ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag acagcgaggt cacacgccga
     2581 atttttaatc atggcgtgac agccttgcgt gccttagccg caaaacaggg ctggcaaatt
     2641 caggagcagg ctgcactggt ttccgccagc gggccggagg gcatgttgag cattgccgcc
     2701 ccggctcgcg acctcaagct cgccaccatt gagcttgaac ataatcatcc tctcgggcgg
     2761 ttatgggata tcgatgtcct gacgcccgaa ggcgacattc tctcccgccg cgattattca
     2821 ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag ctgtttgcgc gcgtggaaaa
     2881 aaccatcaac tgaccgattt actcaaccgc atggaggcgc tgctgaacga tgtcgatgcc
     2941 tgcaacgtca actaaaacca caaagcttgc gacgtcatta atcgatgagt acgccctgct
     3001 gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg aaaccaggcc tcgtggatcg
     3061 gattaactgc ggtgcgcata aagatatggc gctggaagat tttcaccgca gcgcgctggc
     3121 gattcagggc tggttacccc gtttcattga atttggtgcc tgtagtgcag aaatggcacc
     3181 ggaagcggta ctccacggat tacgcccaat tggtatggct tgcgaaggtg atatgttccg
     3241 cgccactgcg ggcgtaaaca cgcataaagg cagcattttt tctttagggc tgctatgtgc
     3301 ggcaattggc cgtttgcttc agctcaacca gccggtaacg ccaacaaccg tatgttctac
     3361 ggcggcaagt ttctgccgtg gcctgaccga tcgtgaactg cgtaccaata attcacaact
     3421 gacggcaggt caacggttgt accagcagct aggccttacc ggcgcacgcg gtgaagccga
     3481 agcgggttat ccactggtga tcaaccacgc cctgccgcat tacctcactc tgctggatca
     3541 gggattagat cctgaactgg cattgctcga taccttgctc ctactgatgg ctaccaacgg
     3601 cgataccaac gttgcatcgc gcggtggcga ggggggcctg cgctggctac agcgcgaggc
     3661 gcaaacatta ttgcaaaaag ggggcatccg aaccctcgcc gatctcgatt atctccggca
     3721 gttcgacagg gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg atttgctaat
     3781 tgtcacctgg tttttagcgt ccctaaccgg gcgctgaatc attaatatat ccggagttaa
     3841 ctatgttaaa agaataccgt aagcatgtag ctgagcgtgc cgctgagggg attgcgccca
     3901 aacccctgga tgcaaaccaa atggccgcgc ttgtagagct gctgaaaaac ccgcccgcgg
     3961 gcgaagaaga attcctgtta gatctgttaa ccaaccgtgt tccccctggc gttgatgaag
     4021 ccgcctatgt caaagcaggc ttcctggctg ctgtcgccaa aggtgaagcc aaatccccac
     4081 tgctgacacc ggaaaaggcc atcgaactgc tgggcaccat gcagggtggt tacaacattc
     4141 atccgctgat cgacgcgctg gatgatgcca aactggcacc gatcgctgcc aaagcacttt
     4201 ctcacacgct gctgatgttc gataacttct atgacgtaga agagaaagcc aaagcaggca
     4261 acgaatatgc gaagcaggta atgcagtcct gggcggatgc cgaatggttc ctgaatcgcc
     4321 cggcgctggc tgaaaaactg accgttaccg tcttcaaagt cactggcgaa accaacaccg
     4381 atgacctctc tccggcaccg gatgcgtggt cacgcccgga tatcccactg cacgcgctgg
     4441 cgatgctgaa aaacgcccgt gaaggcatcg agccagatca gcctggtgtt gttggcccga
     4501 tcaaacagat tgaagctctg caacagaaag gtttcccgct ggcgtacgtc ggtgacgttg
     4561 tgggtacggg gtcatcgcgt aaatccgcca cgaactccgt tctgtggttt atgggcgatg
     4621 atattccaca tgtgccgaac aaacgcggcg gtggtttgtg cctcggcggt aaaattgcac
     4681 caatcttctt taacacgatg gaagatgcgg gtgcactgcc aatcgaagtc gacgtctcta
     4741 acctgaacat gggcgacgta attgacgttt acccgtacaa aggtgaagtg cgtaaccacg
     4801 aaaccggcga actgctggca accttcgaac tgaaaaccga cgtgctgatt gatgaagtgc
     4861 gtgctggcgg ccgtattccg ctgattatcg ggcgtggcct gaccaccaaa gcgcgtgaag
     4921 cacttggcct gccgcacagt gatgtgttcc gtcaggcgaa agatgtcgct gagagcgatc
     4981 gcggcttctc gctggcgcag aaaatggtag gtcgtgcctg cggtgtgaaa ggcattcgtc
     5041 cgggcgcgta ctgcgaaccg aaaatgactt ctgtaggttc acaggacacc accggcccga
     5101 tgacccgtga tgaactgaaa gacctggcgt gcctgggctt ctcggctgac ctggtgatgc
     5161 agtctttctg ccacaccgcg gcgtatccga aaccagttga cgtgaatacg caccacacgc
     5221 tgccggactt cattatgaac cgtggcggtg tgtcgctgcg tccgggtgac ggcgtaatcc
     5281 actcctggct gaaccgtatg ctgctgccgg ataccgtcgg taccggtggt gattcccata
     5341 cccgtttccc gatcggcatt tctttcccgg caggctccgg tctggtggca tttgctgctg
     5401 caactggcgt catgccgctg gatatgccgg aatccgttct ggtgcgcttc aaaggcaaaa
     5461 tgcagccggg catcaccctg cgcgatttgg tacacgctat tccgctgtat gcgatcaaac
     5521 aaggtctgct gaccgttgag aagaaaggca agaaaaacat cttctctggc cgcatcctgg
     5581 aaattgaagg tctgccggat ctgaaagttg agcaggcctt tgagctgacc gatgcgtccg
     5641 ccgagcgttc tgctgccggt tgtaccatca agctgaacaa agaaccgatc atcgaatacc
     5701 tgaactccaa catcgtcctg ctgaagtgga tgatcgcgga aggttacggc gatcatcgta
     5761 ccctggaacg ccgtattcag ggcatggaaa aatggctggc gaatcctgag ctgctggaag
     5821 ccgatgcaga tgcggaatac gcggcagtga tcgacatcga tctggcggat atcaaagagc
     5881 caatcctctg tgctccgaac gacccggatg acgcgcgtcc gctgtctgcg gtacagggcg
     5941 agaagatcga cgaagtgttt atcggttcct gcatgaccaa catcggtcac ttccgtgctg
     6001 cgggtaaact gctggatgcg cataaaggcc agctgccgac ccgcctgtgg gtggcaccgc
     6061 caacccgtat ggacgccgcg cagttgaccg aagaaggcta ctacagcgtc ttcggtaaga
     6121 gtggtgcgcg tatcgagatc cctggctgtt ccctgtgtat gggtaaccag gcgcgtgtag
     6181 cagacggtgc gacggtggtt tccacctcta cccgtaactt tccgaaccgt ttgggtactg
     6241 gcgcgaatgt cttcctggct tctgcggaac tggccgctgt tgcggcgctg attggcaaac
     6301 tgccgacgcc ggaagagtac cagacctacg tggcgcaggt agataaaacc gccgttgata
     6361 cttaccgtta tctgaacttc aaccagcttt ctcagtatac cgagaaagcg gatagggtga
     6421 tttttcctag tgtggtttaa gaagtcagtg tgctgcgtat aaaaagctaa taccaggctg
     6481 cctgtcatat cgatttcatg caggataagt agaactccgt ctaatctaga cggagttcta
     6541 ttgtattgat ggctctggta gaagcgtaag tgtacagcga gagctgtatt gacaggaatg
     6601 cgttattcag ttggcagtgt attgcgcagg gggagccgtt cgctgacgta agcggctcgt
     6661 cagaaccgta ttgatattta ctgagcggtc agttcaactt tccagggcaa cagatcacgt
     6721 acccggtttg ccggccagtc ctggatatgt tcaatgacgt aacgcagcca ttttctggct
     6781 cctcattgtt cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac
     6841 cgttgtcgga acccacgaag agccagtttt tccggcctac ggccactccc cgtaaggcgt
     6901 tctctgcgat gttgttgtcg atttccaccc agccattgct gcagtacaca ttcaggccat
     6961 cccactgttt cagcaggtat gcgaacgctt ttgccgtatc cgagtgacgc gacagtgttt
     7021 tcatctgagt ctgtatccag tcatacagtg actgcatcag tggcgcagct ctggcttttc
     7081 ttgctgccag acgctgttct gctgaacatc cccggacttc tgcctcggtg gcatacagtt
     7141 caccgatacg ctgcagggct tccgtggtga tgtcggttgg cactcttaca tgcacatcgt
     7201 ggattcttct ccgggaatga gccatacacg cggcttccgt tactctgccg gattcgtata
     7261 acgcctaagc atcggcctga agcacaccac aaagactggc cttcaggctg tacacgcagc
     7321 caaagcgttt atcgttgaac accacatttt tctggctgat gtcccattct tcacgcagca
     7381 tagcaatcag ttgtggtgtg caggtaagca gcaagcgaaa aggcagttca aaactggtga
     7441 cgtaatccac attcagcagg gcaatacgca gcagttcttc tggccctgcc tctgtctgcc
     7501 gacactcctc cagaacatcc tgctactgcc gacgaagata tggagattca ttcaattctg
     7561 taaaaccgta tttatccgcc agatagtcaa ttcgtgtacg catactgaaa agaattccgt
     7621 ataaaaattc agcagacaaa actttatcag cccataagaa tcaacggaag agccagtaac
     7681 gtaccagatt atcacctggc tattgttgcc ataacgggta acataaggga ctgaacagtt
     7741 gtctttagca caacgaactg atctaatgta cctgaacggt caacctgaaa acagctttcc
     7801 aaccgacatt gtccgccagc gtttatcccc gaccaggtct gttgcagtaa atccaccact
     7861 tgcgaacatt cttcatcaac tgtggcactg acccgcagat ggcaaatgct cgcctggctg
     7921 agcagcaaca ggcatcgtat tgttgcttct ccatgttgtt ccagcaacca gcgtaatacc
     7981 accggcggtg ctggactggc agtgtgatta cgctcagtaa tatgttcttg cacctgtcgg
     8041 taaaaggcag tacagaatgc atgaatctgt tgcccgttga tgtttccttc tgccgaatag
     8101 agcgtgaagt ggactgccag cagacgcggc cagtggtcca ccgcctgctg aacaaaacgc
     8161 cagatatctc ccggctctga aagtaacgct tcggttattt gcacggaata ctactccttc
     8221 agactctgtt aagttttgtt tgttaaaccg gtgcagacct gcaggaaagc acgccagcac
     8281 cggcactgta cgatataaac atccggtacc ggggatacga atggaatgac gaatacgcca
     8341 gaaaagggat aacaaccttc ctcataatgg tgaaatcatt cgctatcggt tacacggtac
     8401 gcgatgtcgc caaaggcagc tggatcgacg aatccacggt cacgctaccg aaagcgccgc
     8461 cgcttaacac cctgcctcgg gcgaccaaag tgccggagcc actgccgccg caggaagatt
     8521 acacctttga aggttaccgc aacgccgacg gcagcgtggg caccaaaaac ctgttgggta
     8581 ttaccaccag cgtgcactgc atggcagacg ttgaggacta cgtggttaaa attatcgaac
     8641 gcgacctgct gccgaaatac ccgagcatcg acggcgtggt cgacttgaac cacctctacg
     8701 gctgtggcgt agcgattaat gtaccggccg ccgtggtgcc aattcgcacc atccataata
     8761 ttgcgctgaa cccaaacttc ggtggcgaag tgatggtggt gggcatgcag tgcggtggca
     8821 gcgacgcgtt ctccggcgtt accactaacc ccgctgtcgg ctacgactct gacctgctgg
     8881 tgcgctgcgg cgcaacggtg atgttctccg aagtcactga agtacgcgac gccattcatc
     8941 tgttaacgcc acgcgccatc aatgaagaag tgggcaggcg tctgctcgaa gagatggcct
     9001 gatacgataa ctatcccgat atgggcaaaa ccgaccgcag cgccaaccct tcgccgggca
     9061 acaaaaaggg cggcctcgcc aacgtggtaa agaaagcact cggctccatt gctaaatcgg
     9121 gtaaaaccgc aattgttgaa gtgctgtcgc ccggtcaaca cccgactaaa cgcggattaa
     9181 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     9241 tcaccgtgca agtgtttacg accggccgtg gtacgccgta cggcctgatg gcggtacccg
     9301 tcattaaaat agcgacccgc accgggctgg cgaaccactg gtttgattta atggatatta
     9361 acgcaggcac tatcgctacc ggcgaagaga ccattgaaga ggtgggctgg aagttgttcc
     9421 actttattct cgacgtcgcc agcgggaaga agaaaacttt ctcggatcaa tggggattgc
     9481 ataaccagct ggcagtgttt aacccggcac cggtggcctg atattctctt catacattaa
     9541 gttgtattat gcccgataac gcttgtttat cgggcatagt gaatcacagc gaagacgcga
     9601 gctccccgac cagaatcact tcaatcccag cctttcgcaa gccttccaga ctatccgcag
     9661 gaatgccttc atcaacaatg atcatgtcga tacgtttagt atcaatgatc ttatgtaaac
     9721 tggaacgatt gaacttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca
     9781 tccggcggtt taaacgggct tcatcttcat tatgcgtgct gacgccgcgc tccagatcga
     9841 tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct
     9901 gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa
     9961 cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt
    10021 cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa tcgaggagca
    10081 cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg
    10141 tgttcagtgc gcttttgtct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga
    10201 tcaacgcgcc accataggca cgcacagcga tcctctgctt ttccagaaac gccagatcgt
    10261 tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc
    10321 cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcct gtcactcgct
    10381 tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt
    10441 tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattggcg
    10501 caagctactc tcgtttcact gactttcatt atgtttcttt tgtgaatcgg atcagaaaac
    10561 cattatcttt cgttttattt ttatctcacc atgacgcagt atgaactgaa acaaaacgaa
    10621 agattaatat cgcagcaatc tgaactggag aggaaagtga aacatctgac agaaatggtg
    10681 aggcagtaca aagcgggcaa aacaaatgga atttatgccg tttgttttgc ccgctttgtg
    10741 ctgcatgggg cgagcgatgt gccggatgag tatgttcgtc gcaccattgg gccaggcgtc
    10801 tgcaaagtca acgttgcaac cgagttgaag atcgccttct ctgacgccat caaagcttgg
    10861 tttgctgaaa atcagcagag caacgatccg tgcttttaca tgcgggttgg catggacacc
    10921 atgaaagagg tggtcagaag caaaatcgcc gtctgcggct cggcaaatcg attacggcta
    10981 ccggcggagg cctgatccaa cagcgtatta cctcaatatt tcaaaataat tataagtctc
    11041 acaaatatga aggcgcgtcc ttaaaccggg tagcgccttc cattatccta aaattcgagg
    11101 agccctatat gacacaaaaa aaatctttta aatcaaaatt atgggagttt ttacaaagtc
    11161 tgggaaaaac ctttatgttc ccggtttcgc ttcttgcctt tatgggattg ctgctgggta
    11221 tcggtagttc agtcaccagc ccttccacca ttactagttt tccctttctg ggcggcgaat
    11281 ttacccagtt gacctttggc tttatcgcta tggtcggtgg ctttgctttt acctatctgc
    11341 cgctgatgtt tgccatggcg atccccatgg ggcttgccaa gcgcaacaaa gcggtcgctg
    11401 cctttgccgg gttcgttggc tacatgctga tgaacatgag cattaattat tacctgacgg
    11461 ctacccacca gcttgccgac cccgccacca tgaaacaggt aggacaatcg atcgtgctgg
    11521 gcattcaaac cctggagatg ggggtattag gtggcattgt ggttggggtt atcacctatt
    11581 ttctgcatga ccgttttcag gacacggttc tgcatgacgc cttcgccttc tttagcggca
    11641 ttcgtttcgt gccgattatt accgcgctca ccctgtcgct ggtgggtctg ttcattccca
    11701 tgctgtggga atacgtcgcg ctgggcatcg cgggtattgg gcatatcatc cagagcacca
    11761 gcgttttcgg ccccttcctc tacggcgtag gcgtgctgct gcttaaacct tttggtctgc
    11821 accacatcct gctggcgatg gtgcgtttta ccccagcagg cggcattgaa atggtaaatg
    11881 gccatgaggt cgccggggcg ctgaatatct tctacgccga gctcaaggcc ggcctgccgt
    11941 ttagcccgca cgttaccgcg tttctgtcac aagggtttat gccgaccttt atcttcggtt
    12001 tacccgccgt ggcttacgcc atctaccgca ccgcgcgtcc ggaaaatcgg ccggtcatta
    12061 aggggttgct gctttccggc gtgctggttt ccgtcgtcac cggtatttca gagccgattg
    12121 agttcctgtt cctgtttatc gcccccgcgc tttacgcctt ccatatcgtc atgtctggcc
    12181 tggcgctgat ggtaatggcc ctgctgggag tgaccatcgg caataccgac ggcggcattc
    12241 tggatctgct gattttcggc gtgatgcagg gaatgtcgac caaatggtat ctgctgttcc
    12301 cggttggtat tgcctggttt gccatctact tctttgtctt ccgctggtac atcctcaaac
    12361 acaacatcaa aacgccgggc cgcgaggtgg atgttcaggg ggcacagcaa gccgtcgagg
    12421 cgaacacccg cgcgcgcgga aaatcaaaat acgatcacga gcttatcctg cgtgcgctcg
    12481 gaggtaaaga gaacattgag tcgcttgata actgtattac ccgcttgcgt ctggtggtga
    12541 aagatatggg ccttatcgat cagcaggcgc tgaaagcggc aggcgcgttg tcagtggtgg
    12601 tgcttgatgc gcatagcgtg caggtgatca tcggaccgca ggtacagagc gtcaaaaccg
    12661 gcattgaagc cttaatttaa caggaggagt gatgtttgat ttcgacaaaa tcattgagcg
    12721 tcaaagtgat aagtgccgta aatgggacca tacctttgtt tgctcgcgtt tcggtgacgt
    12781 cccggagtcc tttatccccc tgtggatagc cgatatggat ttcacctcac cacctgcggt
    12841 gattgacggt ttccggcgca tcgtggagca cggcaccttt ggttatacct ggtgctttga
    12901 cgaattctac gacgcggtca ttgccttcca gcgcaaacgt catcaggttg aggtggaaaa
    12961 gtcgtggatc acgttgacct acggcaccgt atccacgctg cactacacgg ttcaggcatt
    13021 ctgcaaaccg ggtgacagcg tgatgatgaa cacgccggtc tacgatcctt ttgcgatggc
    13081 ggcacagcgc cagggcgtgc aggtactggc taacccgctg cgcgtggagg aaaaccgcta
    13141 tcagcttgat tttaatctga tagaagaaca gctcaaaacc caccgtccaa cgctgtggtt
    13201 cttctgctcg ccacataacc cgtccggcag gatctggcgc gaggaagaaa tacgccaggt
    13261 gtccgatctc tgtcaacgct acggcacgat tctggtggtc gatgaggttc acgctgaaca
    13321 cattctggat ggcaaattcg tcagttgtct cacctctggc tgtgccgccc aggacaacct
    13381 gatcgtgctc acatcgccca acaaagcgtt caatttgggc gggctgaaaa cctcctactc
    13441 catgattcca gacgactcgc tgcgccagcg cttccgccag cagctcgaga agaactccat
    13501 tacctcgccc aatttgttcg gggtatgggg aatcattctg gcctatcaac acggtctgcc
    13561 ctggctcgac gcgctgaacg gttatctgca aggcaacgcc cggtatctgg cggatgccct
    13621 ccagacccac ttcccggcgt ggaagatgat gaacccggaa tcgtcgtatc tggcgtggat
    13681 agacgtaagc gcggatgagc gtagcgcaac gcagctaacc caacatttcg cacggcaggc
    13741 aggcgtggtc atagaagacg gcagccacta tgtacaaaac ggtgaaaact acctgcggat
    13801 taattttggc acccagcgct actggctgga gcagtccatt aaccgaatgc tgaaaaatga
    13861 caaataagga tcttaccccg atgaagaaag tgctcactct ctcactgctg gctctctgcg
    13921 tttctcatgg tgcagcggca gcaaactacg cgctcaataa cgacaatatt gccctcttgt
    13981 ttgatgatac aaactcaacg gtcgtggtga aggacaacaa ggctaaccat ccgctcacgc
    14041 cgcaggagtt gttctttctg acgctgccgg atgagagtaa aatccacacc gcggatttca
    14101 aaatcaagca cgtcgaaaag caggataacg cgattgtcat cgactttacg cacccggatt
    14161 ttaacgtcac ggtgaagctg aacctggtga agggaaaata cgccaacatc ggctacacca
    14221 ttgccgccgt ggggcagccg cgcgacgtcg ctaaaatcac cttcttcccg acccaaaaac
    14281 agtctcaggc tccttacgta gacggcgcaa tcaatagctc tccgatcgtt gcagactcgt
    14341 tctttatcct gccggataaa ccgatcgtga atacctacgc ctatgaagcc accaccaatc
    14401 tcaacgtaga gctgaaaacg ccgattcagc cagaggcgcc ggtcagcttt actacctggt
    14461 tcggcacttt cccggaaacc agccagctgc gccgcagcgt gaaccagttt attaatgacg
    14521 tacgtccacg cccatacaag ccttatctgc actacaacag ctggatggat atcggctttt
    14581 tcactcccta cactgaacag gatgtgctgg ggcgtatgga cgagtggaac aaggagttca
    14641 ttacgggccg cggcgtggcg ctggacgcct tcctgctgga tgatggctgg gacgatctga
    14701 ccggacgctg gctatttggc acggcattca gaaacggttt tagcaaagta cgggagaaag
    14761 ccgacagcct gcacagctcc gttgggctat ggctttcacc gtggggtggc tacaacaaac
    14821 cgcgcgacgt tcgcgtttcg catgcaaaag agtatgggtt cgaaaccgtg gacggcaaac
    14881 tggcgctgtc gggagcgaac tactttaaaa acttcaatga gcggatcatc aagcttatca
    14941 aaaacgagca catcacctcg tttaaactcg acgggatggg taacgccagt tcgcatatca
    15001 aaggcagctc gttcgcctca gatttcgatg catcaatcgc cctgctgcac aatatgcgca
    15061 gcgcaacccc gaatctgttt atcaacctga ccaccggcac cgacgccagc ccgtcctggc
    15121 tgttctacgc tgattctatc tggcgtcagg gagatgacat caacctgtat ggttccggta
    15181 cgccggtgca gcagtggatg acctaccgcg atgccgagac gtaccgctcc attgtccgta
    15241 aaggccctct gttcccgctg aactcgctga tgtaccacgg gatagtcagc gccgagaatg
    15301 cctattacgg gttagagaag gtgcaaacgg acagcgactt tgccgatcag gtctggagct
    15361 acttcgcgac cggcacccag ctgcaggagc tgtatattac cccgtccatg ctgaacaagg
    15421 tgaagtggga tacgctggcg aaggctgcaa aatggtcgaa ggaaaatgcc agcgtgctgg
    15481 ttgataccca ctggattggc ggcgacccaa cggcgcttgc cgtgtacggc tgggcatcct
    15541 ggagcaaaga caaagccatt ctcggtttgc gcaacccatc ggataagcca cagacctact
    15601 atctggattt ggcgaaggat ttcgaaatac cgacaggaga cgcggcgcag tttagtctga
    15661 aagcggtata cggcagcaat aaaaccgtgc ccgttgagta taaaaacgcg acggtgatta
    15721 cgttgcagcc gctggaaacg ctggtgtttg aggcggtgcc cgttaactaa acgcttgtcc
    15781 caatgagcag accgggtaag gcgcaagcgc cacccggcaa aaccggcagc aggggcttat
    15841 tccccctgct tttccagcgc atacttatac aacgcattct tcttcactcc gtggatttct
    15901 gccgccaacg ccgccgcttg cttcaacggc agctcagcct acaacagcgc cagcgtacgc
    15961 agcgcattgg cgggcagttc gtcatcctgg gctttatggc cttcaataat cagcaccatc
    16021 tcgcctttgc gagggttttt atcttctttg atccacgcca gcagttcgcc gaccgacgtg
    16081 ccgtggatgg ttcccaggcg ggtgttcatc acataacggt atagggcatc attcagcgga
    16141 gccattaact ggcagcacgc gtcaacccag ttggagagta atgcacggct cagctcgacg
    16201 ccctaacggg caaagatttc actctgacgg tacagtggca gatgttcgca gtatttcccg
    16261 ttaacacacg ggcaagcaag ccgggacctg cgataccacg ctctatcgga cgggacggcg
    16321 ccggtgcttc aacaatacag tcacattttg tacaggcttt ttttacccga acagtgcgga
    16381 tcactttcag ggcactgctc accagttcca gctgctcagc actgacttcc cccagataat
    16441 ccagctcact gccacactcc tggcaacaac tttcttcagg ttccaggcgg tgtatttcac
    16501 ggggaagatg tgccggtaac ggacgacgat gacgtgactg tcgcaactgg cggggaacct
    16561 gcgggtcatc ctctcgccca ctgtaacgat cgctgtcctg ttctcgttgt ttcagttggg
    16621 actcagcctg ttcaacctca cgctgcagtt tttcagaacg ggtaccgaac agcatccggc
    16681 gcagtttttc tatctgagcc cgaagatgtt ctatttcccg ttcctcctct tcgatctttt
    16741 cttcggcgcg ggccagcgca gagcgcagga aggcctccgt ctcttcaacc agactcagtt
    16801 gctggtcttt ctgacggagg gcttcagcct gctcagagag caacctttcc agttctgcaa
    16861 tgcgaatgag gtatttctga ctcatgaccg tttttataat gcggtcagga gttttttaca
    16921 acattgtcag tgagttaagg cgggatgttt ttggctgacg ccagtccagc ttatcgagga
    16981 gcattgccag ttgcgagcgg gtaatggata ccttgccgtc acgtaccgca ggccagataa
    17041 actggccttc ctccaggtgt ttggtgaact ggcacagacc atcagaatcg gcccagagta
    17101 ttttaacagt gtcacaccgt cggccacgaa agataaacag gtgacctgag aaggggttct
    17161 catccagcac atgttgtacc tgttctccca gaccgttgaa ggatttacgc atatcggtaa
    17221 cgccggcaac gagccagatg cgggtacctg atgggcgtga gatcatcttc ccctcccggt
    17281 cagttcacgg atcaacacag tgagcagctc tggtgaagga ttttccagtg tcattttacc
//
LOCUS       DARNVM010000102        17181 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.102, whole genome shotgun sequence.
ACCESSION   DARNVM010000102 DARNVM010000000
VERSION     DARNVM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.102"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..661)
                     /gene="rlmB"
                     /locus_tag="SI621_003061"
     CDS             complement(<1..661)
                     /gene="rlmB"
                     /locus_tag="SI621_003061"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEI4065742.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AG"
     gene            complement(752..3193)
                     /gene="rnr"
                     /locus_tag="SI621_003062"
     CDS             complement(752..3193)
                     /gene="rnr"
                     /locus_tag="SI621_003062"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEI4065743.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3232..3657)
                     /gene="nsrR"
                     /locus_tag="SI621_003063"
     CDS             complement(3232..3657)
                     /gene="nsrR"
                     /locus_tag="SI621_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HEI4065744.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3862..5160)
                     /gene="purA"
                     /locus_tag="SI621_003064"
     CDS             complement(3862..5160)
                     /gene="purA"
                     /locus_tag="SI621_003064"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEI4065745.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5264..5461)
                     /locus_tag="SI621_003065"
     CDS             complement(5264..5461)
                     /locus_tag="SI621_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEI4065746.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5543..6547)
                     /gene="hflC"
                     /locus_tag="SI621_003066"
     CDS             complement(5543..6547)
                     /gene="hflC"
                     /locus_tag="SI621_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEI4065747.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6550..7809)
                     /gene="hflK"
                     /locus_tag="SI621_003067"
     CDS             complement(6550..7809)
                     /gene="hflK"
                     /locus_tag="SI621_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEI4065748.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7895..9175)
                     /gene="hflX"
                     /locus_tag="SI621_003068"
     CDS             complement(7895..9175)
                     /gene="hflX"
                     /locus_tag="SI621_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEI4065749.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9252..9560)
                     /gene="hfq"
                     /locus_tag="SI621_003069"
     CDS             complement(9252..9560)
                     /gene="hfq"
                     /locus_tag="SI621_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEI4065750.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9646..10596)
                     /gene="miaA"
                     /locus_tag="SI621_003070"
     CDS             complement(9646..10596)
                     /gene="miaA"
                     /locus_tag="SI621_003070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEI4065751.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10589..12436)
                     /gene="mutL"
                     /locus_tag="SI621_003071"
     CDS             complement(10589..12436)
                     /gene="mutL"
                     /locus_tag="SI621_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEI4065752.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRIAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RGGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12446..13783)
                     /gene="amiB"
                     /locus_tag="SI621_003072"
     CDS             complement(12446..13783)
                     /gene="amiB"
                     /locus_tag="SI621_003072"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HEI4065753.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13802..14263)
                     /gene="tsaE"
                     /locus_tag="SI621_003073"
     CDS             complement(13802..14263)
                     /gene="tsaE"
                     /locus_tag="SI621_003073"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEI4065754.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14235..15782)
                     /gene="nnr"
                     /locus_tag="SI621_003074"
     CDS             complement(14235..15782)
                     /gene="nnr"
                     /locus_tag="SI621_003074"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HEI4065755.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRTAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRVVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDFLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15781..16920
                     /gene="queG"
                     /locus_tag="SI621_003075"
     CDS             15781..16920
                     /gene="queG"
                     /locus_tag="SI621_003075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEI4065756.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEERCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16903..16956)
                     /locus_tag="SI621_003076"
     CDS             complement(16903..16956)
                     /locus_tag="SI621_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065757.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3888 a         4917 c         4311 g         4065 t
ORIGIN      
        1 ttcctgccat tgggatgctg atcaactcgt cgcaatgttc acgggtcagg cgacgcatac
       61 cttcaccttc cgcgcccatc accagtgcca gccgtccggt cattttgctc tgatagagtg
      121 tatgatccgc ctcacctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca
      181 tggtgcgcgc aaggttagtc acccgaatca gcggaacgct ttctgccgcg ccacaggcta
      241 cttttttcgc cgtggcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa
      301 cacctgcggc gtccgcgcta cgcagacatg cacccagatt atgcggatcg gttacgccgt
      361 cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct
      421 gatactgccg tcctggcttc acacgggcga taatgccctg atgcacggca ccgtcgcttt
      481 tctcgtcgag atactgacgg ttcgccaact ggataaccac gccctgggac tcaagggcgt
      541 gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac
      601 gttcaggggc gcgctccagc agggcctgca ctgcgtggat gccgtaaatc atttcgctca
      661 ttaatgtact cgttgtttac gttttgccgg gagcgccacg cttgcccggc ctacggataa
      721 ttcaccctct tctgtttgaa gagggtaatg atcactctgc cacttttttc ttcgccgcac
      781 gcttcgcttt ggtcgctgca gctattttct gcgttttcgc cgatggcttt ttcgcttttc
      841 tcgcgtcttt cttcgccgct ttcggcttcg gttttttctc gccgcggaag gcgctgtctg
      901 gctcaaagtt taccttttta ccgacctgac gacgcttgcc gccttttttg ccagcatcgc
      961 cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt tcgctggaga
     1021 tcaggctaaa gtcgatttta cgctcgtcca tattaaccgc ttcgacgcga acttccacgc
     1081 gatcgcccag gcgataagtc tggccgctgg attcacccat caggcgttgc cctacctggt
     1141 caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca atgaacaagt
     1201 cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct ttaaagacgt
     1261 tacctacctg gtcgagcatg aagtcacact tcagccagtc agctacatcg cgtgttgctt
     1321 cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc tcttccatcg
     1381 aataatggta accaccggtt tcagtggtat tgccctgatg cccctgctct ttcgccagca
     1441 gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc gaagtgaagt
     1501 gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttccggatcg taaatcgcct
     1561 gtttcatcga gcgcagcagc atggtttgca gcatttctgc atcaggacga tcggcaaccg
     1621 actctagcaa ctccgcgtaa tcacgcggtt ccggcttgtt accgcccggc agttccagcc
     1681 ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc ttatcgtgaa
     1741 tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag atattcgcca
     1801 gaatcataca ctcttcaatt aatttgtgtg cgtcgttacg ctgggtctgt tcgatacgtt
     1861 caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat gagatcccac
     1921 cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct tcgagatgct
     1981 taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata tgccagactt
     2041 tggtgtaggt cagacgtgcg tgagagctca ttaccgcttc atagaatttg tagccagtca
     2101 ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct acctgcgggt
     2161 taagcgaaca cagaccgtta gagagcactt ccggcagcat cgggataacc tgcgaaggga
     2221 agtacaccga cgtaccacgg ttacgcgctt ctctgtccag cggcgttgga gggcgcacat
     2281 agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt tttttctcgc
     2341 agtaaaccgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc agcggtaaat
     2401 cgcgcagatc aacacggccc gcttttgctt cttccggcac ttcttctttc agcccggcaa
     2461 cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc agagcgatat
     2521 caaccgccat gccggtgccc atattgtcgc ccagcacttc gacgatttta cccaccgctt
     2581 tggtacggcg agtcgggcgt tgggtcagtt caacaaccac cacaaagccc atccgcgcgc
     2641 ccatgatctg atcgggcggg attaagatat cgaagctcag acggctgtcg tcaggaacca
     2701 caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt tttggcacca
     2761 gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc ggctgcgcca
     2821 gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac aaatcatctt
     2881 tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg gtacctttca
     2941 ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc agttgaccat
     3001 cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt
     3061 gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt aaatgttcga
     3121 ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt tcctggaaag
     3181 gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc gtcactccac
     3241 cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc
     3301 cagttccgta agaaaacttt gcacggcctt agaaagtgcc tgtttcaacc gacaggcagg
     3361 tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca gctcgcgcac
     3421 cacatcacca atacgtatcg cactcgccgg tttacccaga cgaatgccgc cattttttcc
     3481 acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga ccatatgatt
     3541 acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc cttctggcaa
     3601 tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta actgcacatc
     3661 aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc agccacaagc
     3721 tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg
     3781 gaaagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg gcaggcaaaa
     3841 tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca tggtttcagt
     3901 acgatccgga ccggtagaga tgatatcgat cggcacaccg gtcagctctt caatacgctt
     3961 gatgtagttc agcgccgcct gcggcaggcc gctacgatct ttcacgccga aggtggattc
     4021 agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt cagctgccag
     4081 cggagtggta gtcacttcac gaccatccgg catacggtaa gccacgcaga gtttcacctc
     4141 tttcaggcca tccagaacgt ccagcttggt caggcagaag ccagacaggg agttcagctg
     4201 taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac gacccgtagt
     4261 tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa acagttcagt
     4321 cgggaacgga cctgcaccca cacgagtgga gtaagctttg aggataccca gaacgtaatc
     4381 aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg tgttggaaga
     4441 tgttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgag caccttcgaa
     4501 catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt caaccaccat
     4561 agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt aatcaaccgc
     4621 ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt ctttcagttt
     4681 ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac gtgccacttt
     4741 atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg cgccacgcgc
     4801 tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac atgcttcaga
     4861 cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca tctctttcat
     4921 cagcgcggct ggagacagca caacaccgtt accgatgatg ctggttacat tctcgcggag
     4981 aatacctgat ggaataagat ggagaacggt tttttcaccg ttaattacga gagtatggcc
     5041 tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca gaagatcgac
     5101 gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt tgttacccat
     5161 ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca gagttacagc
     5221 actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca atcgttttcc
     5281 tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa
     5341 tattatcagg caaattggtc atcgcagaga tcatcttctt ccatgccttc gggtaaagca
     5401 tcggccctaa accttcgagc accaaaacca gggcaagcgc cagccagatt gtcgaattca
     5461 ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg tggctttatt
     5521 gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga
     5581 agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag ctgttctcat
     5641 aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat gcatcagcaa
     5701 acagtttggc tgcttcggca tcgccttcac cacgcatgat gcggccctga cgctcagctt
     5761 ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct
     5821 gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga
     5881 tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg acaacttcaa
     5941 tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc gtctcagccg
     6001 ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta acttcatctt
     6061 ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg
     6121 aatcggtgac gatgtctttc acatccagac gaccaatttc agaacgcaga cggtcagaga
     6181 acttacgttt caacagcact tccgcttgcg aaatgtcgcc accgcccgtt gccaggtagt
     6241 aacgactgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg tctttcttct
     6301 ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg tcgagcattt
     6361 tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc agaggtttgt
     6421 tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat gccgcgctca ccttctttga
     6481 cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg ataactgact
     6541 tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg
     6601 gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg ttgtggagga
     6661 agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc
     6721 gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac ctttatcgtt
     6781 aaccagcact ttgcgggtgt tgcccaacac tttttccatc gtctcgatat acagacgctc
     6841 gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc gcgccacttc
     6901 accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga ggatacgttg
     6961 cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt cacgaatata
     7021 ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta cttcttctgg
     7081 cggacgagct gcctggaagt tgacgtccag cagagtgata cccatgtcat acggacgaat
     7141 cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt ccgtcagaat
     7201 gcggtccatg gtgtatttac cgataacccc gcgtagggcg ctgtcggtag cctgacgcag
     7261 gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtga cgcggtactg
     7321 cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac cagaagcggc
     7381 cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg tcggtttcca
     7441 gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca cgccacgttc
     7501 ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg ctgccgctgc
     7561 gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc
     7621 agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt tgcggaagat
     7681 atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc cctcagagtt
     7741 gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac cgggctgatt
     7801 ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct ttcgacgcca
     7861 gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg atcaacgccg
     7921 gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc agacttacgc
     7981 tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa cgacttctca
     8041 gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg gaaagccgct
     8101 ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa agccagacac
     8161 ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc agcatatcga
     8221 tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca agaaccgtat
     8281 tcaccgcttc gatgttttct tgtacacgta catccgccgc atcaatgacg tgcagcagca
     8341 atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga tcgtgcggca
     8401 ggtggcgaat aaaccctacg gtatctgcca acacggtttc accgacatcc gcgacgtcaa
     8461 tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag acccgcgctt
     8521 cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc agcgaaacag
     8581 taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt tcaactcttt
     8641 ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg gtttcgagct
     8701 gggtttcacc cggaccacgc aaaccaatcc cgcctttctg tctttcaagg tgggtccagc
     8761 cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc aacttaccct
     8821 catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg tcgatgacac
     8881 gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca tggtcaaaaa
     8941 gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct ttaccttcac
     9001 ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc aatgcttcga
     9061 caccggcgga agagaccaga gattcaaact cctggagatc ttccatatct ttgtcttgcg
     9121 taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca aacaagcgta
     9181 taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg taaaaaaaac
     9241 agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg
     9301 ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg atgagaaacc
     9361 gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt
     9421 ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt aataccattc
     9481 accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt
     9541 aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac tttgaacctt
     9601 tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc ctgcgatagc
     9661 accaacaacc tgtaatactt cgtcacgcgc ctgttctggt ttttcactat caagccagtg
     9721 aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc tcgtggcgca
     9781 aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag accacatctg
     9841 gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag
     9901 cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct gctcaattcg
     9961 ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct gatacggtag
    10021 agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga aaaaaacttc
    10081 cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg caaccggatc
    10141 gatctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct gttgctcaat
    10201 tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca gcaatgcctt
    10261 gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg tgatatcggc
    10321 catttccgcc agcgcatcac ggcgaaaatc agcagccgag taagcctgcg acggatcgcg
    10381 aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct tcgccgtccc
    10441 gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta ctggtaaaat
    10501 tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca tcaaaaaaat
    10561 cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt atcgccggat
    10621 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
    10681 gttccacgtc cgccagcaga gttatggcct gcgccattga ccactgcgca tgttcgctca
    10741 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
    10801 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
    10861 cggccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgccaggg
    10921 cagactgcgc tttttctaat gccgattttt cttcgccaga aacttttagc cgcaacggaa
    10981 tcagcagcgg ctgggcgcaa actggcgctt cacccggcgt cagttgcgcc tgacgcagcc
    11041 aacgttctgc cactggcaag gctaaaagtg aaatgttgcc gccgcgctcc agcaacgcac
    11101 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag
    11161 gttcctgcgg ttctggcgct tttggttttt gcatcggcgc gggcgtttgc aaaagctggc
    11221 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg
    11281 gacgactgcc tgaggcgggc gcaggagtgt aacgcggagc tactggttca cgaactgccg
    11341 gttcagcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggca cgcggtgcag
    11401 gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
    11461 cgccctgata gataaagtca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
    11521 cagggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac acaaatgccg
    11581 gttgctgatc ggcccccagt ttgtcttcac aggcctggcg gatcgcgtga ttgatcaggc
    11641 gatcgcgcat catgcgacca ttcacatagc aatactgaat ttccgccagt gcgggcgtgg
    11701 tgtgattcgg atctgccacc cagccgcgca gcgtaagatc gccgtgttgc cattcaatcg
    11761 ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc
    11821 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg
    11881 tgacgtcgaa acgcgccagc gcaatgcggc gaataatttc atcgatatgg ttaaattcgg
    11941 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
    12001 ccagcgtcgt tcccaccgga tgcgccgccg gtttaaccgt tacgtccata tcgcgccctt
    12061 cggcataggc ctgccaggct tcctgctgtt ctgcgatgcg tgaagtgagc gtcaggcgcg
    12121 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc aaggctgata atggcttcga
    12181 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
    12241 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga
    12301 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt ttccaccagt tctttgacta
    12361 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
    12421 agacctgaat tggcatcaat cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg
    12481 tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc gcaaggaaat
    12541 aattacgcag gcctttgtaa atggcttctg ccagctgttg ttggtaatcg tcgctcgcca
    12601 gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact gatgggatat
    12661 ccggcgaacg cagaacgcca aggctggcgt gttctggtcg acgtttatgt atttcgccaa
    12721 tgcgttgcaa ctggctgatc atactggtcg ctacatcata ccctacacgc tgggaatggc
    12781 cgaactgtaa atccagcaca gcctggctta aataggggtc agactgactg ttcgccagca
    12841 catcgcccgc tccgcccagc agctccgact gtttctcgtg ctgctccagc cagctggcca
    12901 tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc gcactgcggt
    12961 ttggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta cgtgccacat
    13021 cgctgcgccc catcaccgaa ataaagtaat ccccatcacg agttaaaacg cctttaaaca
    13081 tcggatcgtc attgagcaag gtacgtaatt tacgcgcaat ggcgatggtg acatttttct
    13141 cccgcgtacc gccggggccg atagcgccag ggtcctgacc gccgtgtccg gcatcaatag
    13201 cgatgataat tttatcgcca gtgttcgccg tcgcgcgcgc tgccggacgc gttaccgtat
    13261 tactgctgat aacacctgta gtgcggttac tttccgtttt aaacggattg cgcgccggtt
    13321 cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc acaggcggcg
    13381 gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat
    13441 tctgccgctt caccgcttcg gttttaccat tttcggtaag atcgaccacc agccgtagcg
    13501 tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac cagattattg ccgctgaaca
    13561 acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc
    13621 tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgtt
    13681 ggttgccgtt agaaacctga atatcagaga gcgtcgcggc acccacctgc gcgcacagca
    13741 gcagcaacgt cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag
    13801 gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg
    13861 cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac
    13921 ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga
    13981 tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgataaacca
    14041 ttaagttgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct
    14101 gatggcccaa agcctgcaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc
    14161 catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta
    14221 atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact
    14281 tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt
    14341 ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc cgcacaggct
    14401 gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg
    14461 cccataccgc cgctcgccat gcctgcattc ccggcatcaa taatgcctaa agcgtcagga
    14521 tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc
    14581 agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac
    14641 cgtgcggcct caccaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt
    14701 gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt
    14761 tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcc
    14821 gcccattcca ggctttcggt aagaaagtcc atcgtcagtt catgcaccat caattccggt
    14881 cgtgcagtca gcagcggcgc aatgttttca ctgcgggtca atactcggac taaaccagca
    14941 ccagcacgca gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgccg
    15001 ccaataatca ccactctccc gtgatcgcct ttatgcgatg tcgggcgacg cggtttcagc
    15061 cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccag
    15121 ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg
    15181 agcaagcctg gtttcagcgc aataaaggta atggtgtgat cggcgttgat caccgcgcct
    15241 ggcgtagcgc cggtttcagc cagcaggccg gaagggatat caaccgccac aatcggcgca
    15301 ggatgggaat tagcgtggtc gattaactgg ctaatggatt cgcggggtgc ttgctgcaag
    15361 ccggtaccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa
    15421 gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc
    15481 aacggtttgt cactttcctg ggccagcagc gtgacctcaa taccgaccgc tttggccagt
    15541 cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg
    15601 cgggcgtcag gataagccgt gcgacacacc tggaatgcgg cttcgccagc gcgaagcatc
    15661 agctcataga gtgtgagtcc cagcgcatct gccgcctcgc gttctccgcg gcggatatcg
    15721 tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc
    15781 atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa
    15841 ctgggctttc agcaggtagg tattaccgat accgatctca gcgcgtccga gcccaaactg
    15901 caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcgcg tcacggtatg
    15961 ctgcgtgctc gtcctcacga attattgccc ggtacgctgc gcgtgatcag cgtgcggatg
    16021 aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaatcccaa actcggctat
    16081 gttagccgtt atgcgctggg ccgtgactat cacaagcttc tgcgcaaccg actcaaaaag
    16141 ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct
    16201 gcgcctattc tcgagcgccc gttagctgaa aaagcaggac ttggctggac aggtaagcac
    16261 tcacttatcc tcaatcgcga agccggttcg ttcttctttt taggcgaatt gctggtcgat
    16321 attccgctgc ccgtggatca accagtcgag gaaagatgcg gtaaatgcgt ggcctgtatg
    16381 acgatttgcc cgaccggtgc catcgtcgag ccatataccg tagatgctcg ccgctgcatt
    16441 tcttatctga cgatagaact ggaaggggcg atcccggaag agttacgccc gttaatggga
    16501 aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcacaa
    16561 ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag
    16621 ttattcgcct ggagcgaaga gaagttttta aaagtcacgg aaggatcggc gattcgccgt
    16681 ataggtcacc tgcgctggct gcgtaatatc gccgtggcat taggcaatgc cccctgggat
    16741 gaaacgattc tggcggcgct ggagagtcgc aaaggtgagc acccacttct tgatgagcac
    16801 atagcgtggg cgattgcgca gcaaatagag agacgaaatg cgtgcgtggt cgaagtgcaa
    16861 ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga
    16921 ttcatccaca gcttgtgaat aaaaataaaa acgcattgca attcaagacg aataaaatcg
    16981 tcaagtgatc gactcgacaa aacgaaatct taatttttaa cttatctatc aatagattaa
    17041 aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacggcct gaggctgtgg
    17101 ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc
    17161 gctgtggata ttttattgag a
//
LOCUS       DARNVM010000103        17077 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.103, whole genome shotgun sequence.
ACCESSION   DARNVM010000103 DARNVM010000000
VERSION     DARNVM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.103"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            1..468
                     /locus_tag="SI621_003077"
                     /pseudo
     CDS             1..468
                     /locus_tag="SI621_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(573..1622)
                     /locus_tag="SI621_003078"
     CDS             complement(573..1622)
                     /locus_tag="SI621_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HEI4065758.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVVALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWVQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAMDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFAIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWAL"
     gene            complement(1717..3693)
                     /locus_tag="SI621_003079"
     CDS             complement(1717..3693)
                     /locus_tag="SI621_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEI4065759.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFADNTDFTDAHKGFIAPIPQDVIKGEQGNVIWDPQHYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITVVDPLVSAETAKVGMDLY
                     YKNRGKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQIETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKATQDYDQGNFRWVAQVVSKVVFADPNNQAARDLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKGDVKITGNEEKLNELLGYMDNFEFWFNIVTP"
     gene            3999..4226
                     /gene="pptA"
                     /locus_tag="SI621_003080"
     CDS             3999..4226
                     /gene="pptA"
                     /locus_tag="SI621_003080"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HEI4065760.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(4230..4799)
                     /locus_tag="SI621_003081"
     CDS             complement(4230..4799)
                     /locus_tag="SI621_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEI4065761.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            4974..5819
                     /gene="nhoA"
                     /locus_tag="SI621_003082"
     CDS             4974..5819
                     /gene="nhoA"
                     /locus_tag="SI621_003082"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HEI4065762.1"
                     /translation="MTPILNHYFARINWLGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVEVEGEKWIADVGFGGQTLTAPIRLVPELVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHTVEQRNLPDVASLYAVMQEQFGLGVDDVKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(5903..6796)
                     /locus_tag="SI621_003083"
     CDS             complement(5903..6796)
                     /locus_tag="SI621_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HEI4065763.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGPPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLHVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEF"
     gene            complement(6875..7555)
                     /gene="narI"
                     /locus_tag="SI621_003084"
     CDS             complement(6875..7555)
                     /gene="narI"
                     /locus_tag="SI621_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEI4065764.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMIILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(7552..8247)
                     /gene="narW"
                     /locus_tag="SI621_003085"
     CDS             complement(7552..8247)
                     /gene="narW"
                     /locus_tag="SI621_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEI4065765.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREASWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(8247..9791)
                     /gene="narH"
                     /locus_tag="SI621_003086"
     CDS             complement(8247..9791)
                     /gene="narH"
                     /locus_tag="SI621_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEI4065766.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(9788..13528)
                     /gene="narZ"
                     /locus_tag="SI621_003087"
     CDS             complement(9788..13528)
                     /gene="narZ"
                     /locus_tag="SI621_003087"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HEI4065767.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDSVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(13623..15011)
                     /gene="narU"
                     /locus_tag="SI621_003088"
     CDS             complement(13623..15011)
                     /gene="narU"
                     /locus_tag="SI621_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HEI4065768.1"
                     /translation="MALQNEKTSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(15323..16579)
                     /locus_tag="SI621_003089"
     CDS             complement(15323..16579)
                     /locus_tag="SI621_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEI4065769.1"
                     /translation="MKFPSIFNKINPQSIQQHPEKNELNWMLELNQWKAERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDENQLKHFDATTY
                     DRLINLSLNSNALESINFPQGRNVSITHISMNNNSLRNIDMDRLSSITYFSAAHNQLE
                     FVQLECCERLQYLNLSHNQLTDIVAGNKDELLLLDLSHNKLTSLHNTLFPNLNTLLIN
                     NNLLSEIKIFFSNFCNIQTLNAANNQLEKINLHFLTYLSSIKSLRLDNNKITRIDTKN
                     TSDIGTLFPIIKQSKNLNFLNVSGENNCPTIQLMLFNLFSPALKLNTGLAILSPGAFE
                     IHSEGVDVDNELFHYTIKEAYTPYNIHTYKTEEVVNQMNIKIKNMTLDEINNTYCNND
                     YYNEAIREEPIDFLDRSFSSSSWPFQ"
     gene            complement(16641..>17077)
                     /locus_tag="SI621_003090"
     CDS             complement(16641..>17077)
                     /locus_tag="SI621_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine Rich Repeat family protein"
                     /protein_id="HEI4065770.1"
                     /translation="TAVRNIIDDTNEVTIELSYNLAITNIDTSDEHLVEVSENSEGNH
                     IKDNDSMSIRYRSKYYSREYALIEEETIFSDAELKAILPMHRMYGVGDYKSNSSSLPS
                     HSGLKDPTGTPVCYYIHNEDKPSLGYGSTPNNWLSQSFSTEL"
BASE COUNT         4394 a         4123 c         4142 g         4418 t
ORIGIN      
        1 agcctggaaa gtggagcata aattatcgga tattctactg ttgactattt gtgccgttat
       61 ttcgggtgca gaaggctggg aagatataga ggattttggg gaaacacatc tcgatttttt
      121 gaagcaatat ggtgattttg ataatggtat tcctgttcac gataccattg ccagagttgt
      181 atcctgtatc agtcctgaaa aatttcatga gtgctttatt aactggatgc ttgactgcca
      241 ttcttcagat gataaagacg tcattgcaat tgatggaaaa acgctccggt actcttatga
      301 caagagtcgt cgcaagggag cgattcacgt tattagtgtg ttctcaataa tgcacagtca
      361 gattccttgg aagatcaaga cgaatgaaaa aaacaatgag attacagctg tttctgaatt
      421 tcttaacata atgggtatta aaggaaaaat aattgcaccg atccaatgtg ataccagaaa
      481 gacatcgcta agaagatagc tatagacaat tattaccctc cgtcagccct tgttaagcga
      541 ggagagacgt aatctgtcca gtcccctaat gcttataaag cccatcgatt tcgcccactt
      601 tctttcgccc ggtataacgc agcgtcggca tctgaaataa tttgttcagc cgtttttgcg
      661 ggagcagaac aggtaatccc ctgactaacc gtaacaaatg cattaacagc agatgcctga
      721 tgtgggatag ctgccagttt aagttcttgc ttcactctgg ctgctaccgt ctctgcttcc
      781 ggcaatgttg cattaaaaag tatgatggca aactcttcgc caccataacg gacaacaaca
      841 tcctccgact ggcgagttga acgctgtaga gcgtcggcaa cttgtattag acaacgatcc
      901 ccagcaggat gcccgtaagt gtcgttgtaa cgtttaaaaa aatcgacatc cagcataata
      961 agagcaaatg acccgccgtt atccatcgcg tcgtgtaaga tactgttcat tgcacgtcgg
     1021 tttgctatcc cagttaaagg atcacgatgt gctaatgcat caaggcgatt aattaactgg
     1081 ttgttataac tgttttgtac ccatgcttct tcaaaccaac gtaacaaaat gagtcgccca
     1141 ccatatagcg ccagtgaaaa aatacaccat acaacagcaa aatgaatatt gatctgttga
     1201 ttaagcataa gactcgctat tggagtggtg aaccataagg gaatgacaaa tgcgagcaat
     1261 gatggggtat ggaaataaag tgcgacaacg ccactaagca ttaataatac acatagtgga
     1321 taagcaaact gcagcgtcca gtacgcaata aagtgataac tacaccatga ccatagcagg
     1381 ctcagggcaa ctaatagcac ctggataggt aatgtttttt gccgagggat gagtagtaaa
     1441 ccactgcaaa tcagcagaat acccgttgca gatatatcaa tagaatgtat aaggcttttt
     1501 tcatttaacc agggtggagt aaggtcatta aacagaacac ggcgcagcaa aattatcaat
     1561 gcaaaactga cattgataaa agcaaaccat ggcaaactaa tcgtcatgcc gcgaataacc
     1621 atatcgcggc gggttttcca tgagtgatga gtttttcttc gttcagccat tttttaacca
     1681 aaaaagggca ggatacctgc ccggtatgca gtaaatttat ggtgtcacta tattgaacca
     1741 aaattcaaaa ttatccatat aacctaacag ctcgttgagt ttttcctcat tgccagtaat
     1801 tttgacatct cctttggccg tggcttcttt cagcgtctct tctttcagta caattttgtt
     1861 caatacatca cgagacagag tgatagtggc atcagcatcc gaagcctcta caccagcggt
     1921 atgattgaga acaccatttt caagctccac tttataggtg ccgccatctt caccaaaatc
     1981 gaaattcaac acggttttgg catctgccgc tttctcacca ttaatatgga cggcaagata
     2041 atcaaagaac atctccggcg tcatcgcacg cacggtgtct gggctggcag tatttggtgt
     2101 tggtaatttt tgtacgccat tacgcaattc ctgtgcacca gtgagataga aattacgcca
     2161 cggcccagat tcagcctgat agcctaactg ctcaagcgca tcagcttcca gatctctcgc
     2221 tgcctggttg tttggatcgg caaacaccac cttactaaca acttgggcaa cccagcggaa
     2281 attaccctgg tcataatcct gtgtggcttt ctgtaaaatg gcatcagcac cgcccatata
     2341 ctcgacgaat ttttttgcgc cttcttctgg cggcagttca tcaagcgtcg cggggttacc
     2401 atcaaaccag ccgagataaa gcacgtaagt tgctttcacg tcatgactta ccgaaccgta
     2461 atagccgcga ttcgcccagg tgttagccag tgaagagggc agtttaaagt ttgcggcaat
     2521 ttcatcacgg gtcatccctt gattagccat gcgcagagtt tgatcattga tgtaacgata
     2581 caaatcacgc tggcttttaa gcagtttgac cacgttatcg ttgccccagg ttggccagtg
     2641 atgttgagcg agaataatct cggctttatc accccacaaa ttgagagctt cattaatata
     2701 ttttgaccac ggcagcggtt ggcgaatttt agccccacgc agcgaatagg tgttatgaag
     2761 ggtgtgcgtg acatcttcag cggtttcgat cagtttcttt tcttcaataa accacagcat
     2821 ttcagaaggc gcttcagaac ctggcgccat caggaaatca taagtcaacc cgtcaatagt
     2881 ttctatttgt ccggttttgg tgatgtagtt agtaggagca atcaacgtga ctgttcctgc
     2941 tgaagtagtc gttcccagtc cagcccccac ctggccttta acatcaggtt tcaataagtt
     3001 gccatacata taactggctc gtcggctcat aacattgcca gccatgatgt tttcggaaac
     3061 ggcttcctcc aaaaatccgg ctggcgcata gattttaact ttgcctgatt taacgtcagc
     3121 ttcatcaatt acgccacgaa caccaccgta gtgatcgaca tgactgtgtg tatagataac
     3181 cgccactacc ggttttttgc cgcgattttt gtaatagaga tccataccca ctttggctgt
     3241 ttctgctgaa acaaggggat caacgacggt aataccctct ttgccttcaa tgatggtcat
     3301 atttgataga tcgagattgc gaatctgata gacaccttca gttacttcga ataacccgct
     3361 gatatttatc agttgcgcct gacgccataa actcggattc accgtatcag gggctttatc
     3421 gccttctttg ataaaagcgt aatgttgtgg atcccagata acattccctt gttcgccctt
     3481 aatcacgtct tgagggatag gggcaataaa acctttatgg gcatcagtaa agtcagtgtt
     3541 atcggcaaac ggcaactggt tgtagagtaa attgtttgct tgttgcgtgg ctgcggtggc
     3601 gtctttagga gcttccgccg cgaggagagg taaagaactg gtggtgaata atcccgttat
     3661 tagcagactt ttaacgatat gattaagctg catcttaagc gtctccagaa tggattttta
     3721 ttgggggtaa ttacggatga aaatttttag tgagggcaag aataactata atgggcgtta
     3781 acaaattggc aagccgggaa attgatgttt tcttaatggc atgttggcga agaatatatt
     3841 ctgcaagtta acgttttcat tggcgcttta tttaaaagtg tgtagtacaa atatgtacaa
     3901 tttcaagtag gcattattgc cgtaaaaacg cattaccgga cacacgacat tcacaacgtg
     3961 tgttaatttt actgacatct ttcaaaagga gcacaatcat gccgcacata gacattaaat
     4021 gttttccgcg tgaactggac gaacaacaaa aagcagcact tgctgcagat attaccgacg
     4081 ttattattcg ccatctgaac agtaaagata gctcgataag catttctcta cagcagattc
     4141 aaccagaatc ttggcaagct atctgggatg ccgaaatcgc gccccaaatg gaggctttga
     4201 taaagaaacc cggttacagc atgtaatgct taacctgccg taacacgctt gccgctggta
     4261 acaaacgtgc cagcacctaa atgatgcaac gtattgagtt tgtcatcatc aaactgccag
     4321 cggccttcga caaatactcg tgcgtctgcc gctgcagata ccacttcgcc aaatagcgta
     4381 tcgtattttt cttgcgcgga agttgcaggt agcaggcgac actccatcca cgccagacat
     4441 ttttcctcga ccagcggcaa accaagcacc ggacctttca caaccggaat accatagcaa
     4501 ttaaacttat cttcatcacg ccccgacaca cttcccaccg cccacgtcca gttagttgct
     4561 gcaacacccg ggataacgat gccaaactta ccattacgtt caatcaactc cctggtccat
     4621 gtcgatttat ctaccacaat cgccacgcgt ggcggttcaa actccaccgg cattgaccag
     4681 gcggctgcca taatgttacg ccgctgggat tgctcatcga aactggtgat caagacagtt
     4741 gggccatgat ttaacagacg gctggcatgg tgtaattcga cagggatgaa tcggctcacg
     4801 gtatcgcctc gctatgaagg gtgttttaaa agcgtagctc gtaacgcaat aagtaacgaa
     4861 attaacggga ttggcgattt gcgaacgtga tgcatgtccg cgatcgcaca aaatagccgg
     4921 tgcagcgtct attccaggtt ataagttgag aaaaccacta agggaaacgc ctgatgacgc
     4981 ccattctgaa tcactatttt gcccgtatta actggttggg agctgctgcg gtcaatatag
     5041 atacgctgcg tgcattgcac ctgaaacaca attgcaccat tccgtttgaa aacctcgacg
     5101 ttttgctgcc gagggaaata cagcttgatg atcaatcgct ggaagagaaa ctggtgacag
     5161 cccgtcgtgg tggttactgt tttgagcaga atggcgtgtt tgagcgggtg ttacgcgagc
     5221 tggggtttaa cgttcgcagc ttgttagggc gcgtagtgtt atcaaatccg ccagcattac
     5281 cgccgcgcac ccatcgtttg ttgttggtgg aagtggaagg ggaaaaatgg attgctgatg
     5341 tcggtttcgg tggacagacg ctaaccgcgc cgattcgatt agttcccgag ctcgtgcaga
     5401 ccacgccaca cggagagtat cggttgttgc aggagggtga tgattgggtg ttgcaattta
     5461 atcatcatca gcactggcag tcgatgtacc gttttgatct ctgcgagcag caacaaagcg
     5521 attatgtaat gggcaatttc tggtctgcgc actggccgca gtcgcatttt cgccatcatt
     5581 tgctgatgtg ccgccatttg ccggacggcg gcaagctgac actgaccaat tttcatttta
     5641 cccattatga aaatgggcac acggtggagc agcgaaatct accagatgtg gcgtcgttat
     5701 atgccgtgat gcaggaacag tttggtctgg gcgtggatga tgtgaaacat ggctttactg
     5761 tggatgagtt agcgctggtg atggctgcgt ttgatacgca cccggaggcg ggaaaataat
     5821 ttatgtcagg ttgccggatg cggcgtaaac gccttatccg gcatacatag acctgataag
     5881 cgaagcgcat caggcaacgg tttcagaatt caattgccca ttcggcatga aacaaaatca
     5941 ccgctgtgcc agaaatggtg actttctccg gttggttatc gcgaattgtc accgtcactt
     6001 caatcacgcc gtcacgccct aatgcgcgcc cctgatggcc tttaacatgc aacacgttgc
     6061 catcgtgggg caatacgttg tgatgtacca accatgcacc catcgggcca ttggcattgc
     6121 cggtcaccgg atcttccaca ataccaatgg caggcgagaa catacgaccg tcggtttcgt
     6181 ttttgcctgg gcggatctgg aacgggaaga aaccattgca gccaatctgt ttgctgatag
     6241 cggtcagcgc attaaggtca ggcgaaaggg cgtcgatatc cacttccggt ttcagtggga
     6301 tcatcacttt tgagtgtcct gtggttgcca cctgaattgg caagcctggc agaatatcgt
     6361 cttcagtgag atgaagcgcg ttgataatcg ccgcacgtgt ttcaccttcc agcggcggtt
     6421 caaagcccgg cggaccttgt tccagcgaaa tacgataatc atcgttgtgc ttttcgatag
     6481 tcacgcgatg ttttcctgcc agtgacgttt gccagatcgt gcaatttcct aaacctaaca
     6541 ccttcgcacg tacatagtgc gcagctactg tcgcgtgacc gcaaatcggc acttcaaccg
     6601 ttggcgtaaa gtagcggatg cgtacatcgc tgtcgtcgct gtgcagcaga aaagcagttt
     6661 ccgaatgacc taattcgcgg gcgataagct gcatttgcgc ttcactgaga ttatcggcgg
     6721 ggaaaacaac accggcagaa ttgccgcgaa acggttgtga ggtaaaagca tcgacgtggt
     6781 acacctgcgg tttcattaac gttctcctgt ggctggagaa ctatcatagc ctgcaagtgg
     6841 ccggagagcg aagggctatc cggccagggt gaaattatcg ccgcgagcgc acaatctgat
     6901 accgacgagt aaagtactca aacggcgcgc tccacacatg caccagtcgg gtgaacggga
     6961 agagcaggaa gatggtcatt cccaacacca gatgcaggcg gaaaacgaac gctacgccgt
     7021 taagcatttc tgacgatcca ccacggaaag tgacaatgct ttgcgcccaa cccaccagct
     7081 tcatcatttc gctaccatca ggatactgtg ctgaaaacgg tatggtactt agccccagca
     7141 gacactgaat cagcaaaatg atcatgatga taatatccgg cgtagtggaa gtggcacgca
     7201 cgcgctgatt tgtcagcctg cgccacagca gtcctgcgcc gccaatcagc gtcaatacac
     7261 cgcaaatacc accgagcacc atcgccatct gctgttttac cgccaccggc agaaaccacg
     7321 cgtacatcca gtgcggcgtt aacatgccga acaggtgccc gaagaaaatc cccaaaatgc
     7381 cgatatggaa cagattcgac catatcacca tcccgcgttt atcgagcatc tgactcgagg
     7441 aggcgcgcca ggtatactgc ccgtagtcgt agcgcagcca gctgccgagg aaaaacaccg
     7501 tcgcacaaat gtacgggtag atatcgtaaa aaaagacgtt cagatactga atcatttccc
     7561 acctcccgca ctgatgtcga catactgcgg cgcgacatcc tggctaaagc gtcgctgata
     7621 ttgattaagc ggcgagctgt cacacgccgt ggcgttatct tcaataaact tcacctgttc
     7681 ctcttcccac accgcatcca gtgcctggcg ggtgtcatcg cgctcctcac tgttcacctg
     7741 ttttgtgaca ctgtcacttg aaagtgtact tcctgccagt tgcaacaggg tgtcaaataa
     7801 cgcgtaccac gatgcctcgc gttgttttaa gcgtccgcca agcagggcga ggatcggcgc
     7861 aacgttgagc aagccttctt tcgcctgatc gtccggcagt acgcttaagt actccagata
     7921 cagcggtaga taatcgggca gttctcgaca atccaactgc aggccgacct tttcatactc
     7981 cgccagcagg tccaccattg cctggccgcg atcgcgggac tcggcatgaa catgttcgaa
     8041 cagcagcagc gatgtggtac gtccacggtc aaacacttcg caccattcgg cctgtttatc
     8101 cagcagcggc gcgttaagca ggttgcgggt aaaatccgta agcatcggtg cgtcgcggcg
     8161 gatcaacgcc agcgcgtcct ccttacattc ccacaacagc tcgtctggat actccatcag
     8221 caggccgatc actttgagga tctgcattat tcgccctccg ctttgtcgcg cacttcggtg
     8281 atgttgatgg catcgatacg gctactgttg aacaggttga atttactgtc cgaaccgtgg
     8341 caaccgtcgc caaaggtaaa accgcagcca ttgcgttctg cgaaggcatc gcccgccatt
     8401 tcccgatggc tggtcgggat aacaaaacga tcttcatagt tggcaatggc gagatagcga
     8461 tacatttctt cgacctgggc gacgctcagg cctacttcgt cgatggcacg agtatcagta
     8521 acgccttcca cggtttgtga acgcatatag tggcgcatcg ccatcatccg tttcagcgcc
     8581 cgcagtaccg gaccggtatc gccggcactc aacatattgg cgagatattg caccggaata
     8641 cgcaggcttt cgatggcggg cagcacgcct tcgcttttcg gcaaaccgcc cgcatctgcg
     8701 taggactgaa tcggtgacag cggaggaacg taccagacca tcggcagggt gcggtattca
     8761 gggtgcagcg gtaacgccag tttccagtcc atcgccattt tgtagactgg cgaacgctgg
     8821 gcagcgtcaa tcacattttg tggaatacct tgtttcaggg cttcttcgat cactgaggga
     8881 tcgtgcggat cgaggaacac ttcgcactgg cgttcataga ggtcaacttc gcgctcggtg
     8941 ctcgccgctt cctcgatgcg gtcggcgtcg taaagcagca cgcccagata ccggatgcga
     9001 cccacgcagg tttctgagca cacggtgggt tggcctgact caattcgtgg gtaacagaag
     9061 atacattttt ctgacttgcc gcttttccag ttgaagtaga tttttttgta cggacaaccg
     9121 ctaatgcaca aacgccagcc acggcattta tcctgatcaa tcagcacaat gccgtcttct
     9181 tcacgtttgt agatagcgcc gcttgggcag gttgccacgc aactgggatt gaggcagtgt
     9241 tcgcacaggc gcggcaggta catcatgaag gtgttttcaa actgcccgta catctccttt
     9301 tgcatggcct cgaagttgcg gtcacgggcg cgtttttcga attcgccgcc caacaattct
     9361 tcccagtttg gcccccagat cactttgtcc atccgtttgc cgtcaatcag tgaacgcggg
     9421 cgagcagtag ggatatgttt gccttctggc gcgctatgca aatgttcgta gtcgaaggtg
     9481 aaaggttcgt agtaatcatc aatctgcggc accaccgggt tggcgaagat tttggtaatc
     9541 acgcccatct tgctgcccag acgcggacgg atcttaccgt tgacatcgcg gacccagccg
     9601 ccttgccact cttcttgatc ttcccagttt ttcggataac caatgcccgg tttggtttcg
     9661 acgttgttaa accatgcgta ctccatgcct tcgcgtccgg tccagacgtt tttacaggtc
     9721 accgaacagg tatggcaacc gatacatttg tcgaggttaa gcaccatgcc gacttgcgaa
     9781 cgtattttca ttttttcgcc tcctgtacct gatcgcgacc ttcatcatcc agccagttaa
     9841 cgttcttcat cttgcggatc atgatgaatt cgtcacggtt cgagccgacg gtgccgtagt
     9901 aattaaagcc ccaggccagc tgcgcgtaac cgccaatcat atgcgttggt ttcggacaaa
     9961 cgcgggtgac cgagttatga atgccgccgc gcatgccagt tacttccgaa ccaggaatat
    10021 tcataatgcg ttcctgggcg tgatacatca tggtcatacc cggcggtaca cgttggctga
    10081 ccaccgcgcg ggcagtcagc gcgccgttgg cgttaaacac ttccacccag tcgttatcga
    10141 caatggtcag ttcacgggca tcggtttcgc tgatccagac aattggtcca ccgcgagaga
    10201 gcgtcagcat tagcaggttt tcactgtagg ttgagtgaat gccccatttc tggtgcggcg
    10261 tcaggaagtt aagtgctttt tccgggaagc cgtttggcgg tatctggcgc atttcactga
    10321 cgctacgggt gtcgataggc gggcgataag ccaccaggct ttcaccaaag gcacgcatcc
    10381 acggatgatc ctgataaagc tgctggcgtc ccgacagcgt gcgccacgga attaactcat
    10441 gaacgttggt gtatcccgcg ttataggaga cgtgatcgct ttccagacca gaccatgtgg
    10501 ggctggagat aattttacgt ggctgcgcct gaatatcgcg aaagcgaatc ttctcgtcct
    10561 ctttgtgcag cgccagatgg gtatgttcgc gtccggtgat ctcgcccagc gcctgccacg
    10621 ctttgactgc aacatgaccg ttggtttccg gtgccagagc cagaatcact tctgatgcgt
    10681 caatggcggt gtcaatcagc ggacgcccct gtgccgggcc ttcacgcttg gtgtagttga
    10741 gtttgccgag gaaatcgatt tcatcctggg tattccacga aatcccttta ccgccgttgc
    10801 caagtttgtc catcaatggc ccgagcgagg taaagcgttc ataggtagca gggtagtcgc
    10861 gttccaccgc cacaatattg ggcgcagttt tacctggaat cagatcgcat tcgcctttgc
    10921 gccagtcgag cacttcacac ggctgtgaga gttctgccgg agagtcatgc agcagtggtt
    10981 gtaataccac gtcggtttct ttgccaagat ggcccacgca cacttgcgaa aaggctttgg
    11041 caataccttt gtagatttcc cagtcgctgc gtgattccca cgccggatcg accgccgcag
    11101 aaagcggatg aataaacgga tgcatatccg aggtgttcat atcgtctttt tcgtaccagg
    11161 tggcggtggg cagaacgata tcggagaaca ggcaggtact ggacatacgg aagtcgagcg
    11221 tcaccagcag gtcgagcttg ccttcaatcg ccgcagtttg ccactcgact tcttctggtt
    11281 tgatcccgtc gctggcaccg agttcctgac cctgaatccc actttcggtt cctagcagat
    11341 acttctgcat atactcgtgg cctttgccgg aggagccaag caggttagaa cgccagacaa
    11401 acaaattacg cggatgattg ctgccgctat ctggttgttc gcaggccatg cgtaaatcgc
    11461 ccgatttcag cgcctgggcg gtaaattctg tgggggataa tccggccttg tcggcttcag
    11521 ctttaatccc gagcgggtta cgccccagct gcggcgcaga aggtagccag cccatacgtt
    11581 cggcacgaac gttgaaatca atcagatgac cgctgtactt actggcatcg gcgagcggtg
    11641 aaagtaactc ctgagcagag actttttcat agcgccattg gctggaatga ttgtagaaaa
    11701 acgaggtgct gttcatctgg cgcggtggtc ggttccaatc gagcgcaaag gccagcggca
    11761 accagccagt ttgcgggcgc agtttttcct gaccgacata gtgtgcccat ccgccaccgc
    11821 tttgcccgac acaaccgcag aagatcagca tattgatcat tccccggtag ttcatgtcca
    11881 tgtggtacca gtggttaaca cctgcgccga ggataatcat cgagcgccca tgcgttttat
    11941 gggcagtatc ggcaaattca cgagcgatgg tttcaatata ctggcgcggc acaccggtaa
    12001 tttgctcacc ccaggctggg gtgtacggtt tgatttcagc gtaatctttc gcgctgtttt
    12061 catcttccag cccgcgatcg aggccgtaat tcgccagtac caaatcataa acgctaacca
    12121 ccggacaagt gctgccatcg gctaatgtca ggtttttaac gggtaattga cgcaccagga
    12181 ccggattgtg ttttacgcta cgaaaatgcg gattttcaat gccgccaaag taggggaagg
    12241 ccacgcctgc aacggagtca tgttgaccaa gcagggttaa tgacaattcg gtttccgtac
    12301 cggcggcaat agattccaga ttccatttgc ctttttctcc ccagcgaaaa ccaatcgaac
    12361 cgttcggcac caccagttca cctgcggtat taaccgccac ggttttccac tgcggattgt
    12421 tgctttcgcc cagtccatcc accaggtcag atgcgcggac catgcgcccg ggaacgtagc
    12481 tgccgtcgtc gcgaggttcc agcattacca gcatcggcat gtcgctgtag cggcggcagt
    12541 agttgataaa ataatcgctg ggattatcga gatgaaactc ttttaaaatg acatggccca
    12601 ttgccattgc cagggcacta tcagtgcctt gtttcggtgc cagccactgg tcgcacaatt
    12661 tggccacttc agagtagtca ggggtaatgg cgatggtttt agtgcctttg tagcgtactt
    12721 cggtaaagaa atgggcgtcc ggcgtacgtg tctgcggtac gttagacccc caggcgatga
    12781 tgtaactgga gttataccag tcggcagatt ccggcacgtc ggtttgttcg ccccaggtca
    12841 tcggcgaggc gggcggcagg tcgcaatacc agtcgtagaa gcttaaacag gtgccaccta
    12901 atagtgaaag ataacgtgtt ccggcggcgt aagaaaccat cgacatcgcc gggatcggcg
    12961 agaaaccggc aacgcgatcc gggccgtagg ttttgatggt ccagacgtta gcggcggcaa
    13021 tcagctggtt tagttcctgc cagttggagc ggataaagcc gccgcgtcca cgtacttgtt
    13081 tgtagctcag gcacttttgc ggatcgttca taatcgatgc ccacgccagt accggatcgc
    13141 tgtgttgctt gagggcttcg cgccacagtt caatcagtcg tttacgaatg agcgggtatt
    13201 tcagacggtt agcgctgtag agataccagg agtaacttgc gccacgcggg cagccgcgag
    13261 gttcatgatt gggcaggtca gggcgagtgc gcgggtagtc ggtctgttgg atttcccagg
    13321 tcaccagacc atttttaacg tagattttcc agctacagga gcctgtacag ttaacaccgt
    13381 gggtggaacg cacgattttg tcgaactgcc aacgctggcg atagctgtcc tcccagtcgc
    13441 ggttgctatg catcacctgt ccgtgaccat cggcaaaggt ttcgcccttt tgtttgaagt
    13501 agcgaaagcg atccaaaagt ttactcatga cataactcct gctccaggaa tgaattggga
    13561 tctgatgcct tgtcggatgc gacacggttg tagcctgata agacgtgtca ggcatcgatc
    13621 tcttattttt ggctgaactt ccgccgacca taaaccagcc aggtcagcag cacacaaacg
    13681 atgtagaaga ttaaaaacac tttcatcgcg cctaccggag agccggtcat attgagcgac
    13741 atgccaaacg cctgcggaat gaaaaagccg cccactgcgc caatggctga gataaagccc
    13801 agagccgccg ccgtttcggt gacggcttct ctctgagctt gctcatcact accgcctttc
    13861 atcttcaccc gataaatggt tatctggcga aagatgacgg cgatcatctg gaaagtagaa
    13921 ccacttccca gacctgcggt cagaaacaac cccataaata cggcgtaaaa tgcgatgaaa
    13981 ttaccggagc ctgtgcccgg taaggtaagg aacagcaggg cgctgaaaat cgccataaaa
    14041 ataaagttga tcaacgtcac ccgcacgccg ccgaatttat cggaaatagc accaccaacc
    14101 gaccgcgcga tggcaccaat aaatgggcca aagaacgcca ggcgcagaat attcacatca
    14161 gggaactgag ttttcgccag catggcaaaa cccgcagaaa aaccgataaa cgaaccgaag
    14221 gtggcaaggt aaagcaggct cagcagccag agatgcaggc gttgtaacac tggcagctgg
    14281 tcggcaattg acgcgcgtga actggcgata tcattcatcc ctgaccaggc ggcgatcgtg
    14341 gcaatcgcca gtaatggcac ccaaatccat gcggcattcg ccagcgacat caccgaaccg
    14401 tcggcctgcg gtacgccatt gacgccgaga aaggcaaata cgggtacaaa aatgaccagc
    14461 ggtgcaacca gctgcatcac acttacacct aagtttccta atccgccatt aatcccaagt
    14521 gcgctccctt gtttggcttt tgggaagaag aaactgatat tgcccatgct cgaagcaaag
    14581 ttcgcacctg caaaaccgca tagcaaagcg ataacgataa atatcccaaa aggagtattc
    14641 ggattctgca cggcaattcc gagccagacg caaggaataa tcaggattgc agtactaaaa
    14701 accgtccatc ggcgtccacc aaatataggc accataaagg agtaggggac acgcaataat
    14761 gcgccagaaa cggagggtaa tgcggttaat aaaaagagtt gatcggtagt gaaattaaaa
    14821 ccgattttat tgagattaac agtaactgcg ctaaatagca tccagacaca gaaggcaaga
    14881 agtagacaac tgactgatat ccagaggttt cttcgagcaa tatgttttcc tttattttcc
    14941 cagaaggccg gattttctgg tttccagtcg cgcaaaagat aacgactagt tttctcattt
    15001 tgcagtgcca tattgttcct cacatgtaca cattggtaat gaaaaaaaga caaaacagga
    15061 ggtaaggcgc aatagccagt tattagaatt aaggatgaat taggtgaagt gctgattgaa
    15121 agaatagata agaaagcgta accgcggggg cagaatgggg attaaacaga cagatatgtg
    15181 ttactaaatg taactaacaa gctaaccatt aaacggatgt taagctatgt atgcatgtca
    15241 tgatccaacc cttcagatga tgaatccaaa gggtttatat tttcaacaag accagacact
    15301 tatagaatta gtgatgattt attcactgaa aaggccatga gctggaggaa aacgatctgt
    15361 ccagaaagtc tatcggttcc tctcttatcg cctcattgta ataatcgtta ttacagtaag
    15421 tattgtttat ttcatctaat gtcatatttt taatttttat attcatctga tttacaactt
    15481 cttctgtttt ataagtgtgt atattatatg gggtatatgc ttctttaata gtatagtgaa
    15541 acaattcgtt atccacatct actccttcag agtgaatttc aaatgcacca ggcgaaagaa
    15601 ttgccaggcc agtattaagc ttaagtgctg gggaaaataa attaaataac atgagctgga
    15661 tagtagggca attgttctcg ccagaaacat ttaaaaaatt taagtttttg ctctgtttta
    15721 ttatggggaa taaagttcca atatcggacg tattcttagt gtcaatgcgg gttattttat
    15781 tattgtccag ccttaaactt ttgatagatg aaagataagt caggaaatga aggtttattt
    15841 tttccaactg attgttcgca gcgtttaatg tctgaatatt gcagaagttg ctaaagaata
    15901 ttttaatttc agaaagcaag ttgttgttga taagtaacgt attcaagttg ggaaataagg
    15961 tattatgtaa acttgttagt ttattatggg atagatccag aagtaagagt tcatctttat
    16021 ttcctgccac aatatcagtt aattgattgt ggctgagatt caggtactgc agcctttcgc
    16081 aacattctaa ttgcacaaac tctagttgat tatgtgccgc actaaaataa gtaattgatg
    16141 aaagcctatc catatcaata tttctgagag aattattatt catagatata tgtgtaatgc
    16201 ttacatttct accttgagga aaatttattg actcaagagc attactattc agactaaggt
    16261 tgatgagtct atcgtaggtg gttgcatcaa aatgtttgag ttggttctca tcaagattga
    16321 tagaggtgaa gttttgcagt cctggtggtt gggtagttaa attaagccct gataaatcaa
    16381 tatcattctg tttcgacaaa aaagcacaat ttatcctttt agcggcttcg tttcggcatt
    16441 ccggacgatg gatttcacct gtaagtatgc gttctgcttt ccattgattt aattcgagca
    16501 tccagttaag ttcatttttt tctggatgtt gctgtatgga ttgtggattt attttattaa
    16561 atattgaagg gaatttcata actgctccgt agtaatactt tcttttctga tttatcaccc
    16621 ttgataaatc aggatgtgtt ttataactct gtagaaaagg attggcttaa ccaattattg
    16681 ggtgttgaac cataacctaa ggaaggttta tcctcattat gaatataata acagacgggt
    16741 gtgcccgttg ggtcctttag ccccgagtgt gagggtagag aagaggaatt tgacttatag
    16801 tcaccaaccc cgtacatgcg atgcataggc agaatggctt ttagttctgc gtcagaaaat
    16861 attgtttctt cttctattaa agcgtactct ctggaataat attttgatct ataacgaata
    16921 gacattgagt cattgtcttt tatatgattt ccttcggaat tctcgcttac ttctacaaga
    16981 tgttcatcgc tggtatcgat atttgtgatt gccaggttat aagataattc aatggtcact
    17041 tcattagtgt catcaattat atttcttact gcagtcc
//
LOCUS       DARNVM010000104        16869 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.104, whole genome shotgun sequence.
ACCESSION   DARNVM010000104 DARNVM010000000
VERSION     DARNVM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.104"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(41..2896)
                     /gene="valS"
                     /locus_tag="SI621_003091"
     CDS             complement(41..2896)
                     /gene="valS"
                     /locus_tag="SI621_003091"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEI4065771.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGTDVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2896..3339)
                     /gene="holC"
                     /locus_tag="SI621_003092"
     CDS             complement(2896..3339)
                     /gene="holC"
                     /locus_tag="SI621_003092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEI4065772.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3473..4984)
                     /gene="pepA"
                     /locus_tag="SI621_003093"
     CDS             complement(3473..4984)
                     /gene="pepA"
                     /locus_tag="SI621_003093"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEI4065773.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5251..6351
                     /gene="lptF"
                     /locus_tag="SI621_003094"
     CDS             5251..6351
                     /gene="lptF"
                     /locus_tag="SI621_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEI4065774.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6351..7433
                     /gene="lptG"
                     /locus_tag="SI621_003095"
     CDS             6351..7433
                     /gene="lptG"
                     /locus_tag="SI621_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEI4065775.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7594..9096)
                     /locus_tag="SI621_003096"
     CDS             complement(7594..9096)
                     /locus_tag="SI621_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEI4065776.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRRR"
     gene            complement(9174..10172)
                     /gene="idnR"
                     /locus_tag="SI621_003097"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9174..10172)
                     /gene="idnR"
                     /locus_tag="SI621_003097"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HEI4065777.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10239..11558)
                     /gene="idnT"
                     /locus_tag="SI621_003098"
     CDS             complement(10239..11558)
                     /gene="idnT"
                     /locus_tag="SI621_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HEI4065778.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHAAPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(11623..12387)
                     /gene="idnO"
                     /locus_tag="SI621_003099"
     CDS             complement(11623..12387)
                     /gene="idnO"
                     /locus_tag="SI621_003099"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEI4065779.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKNIGPIDVLVNNAGIQRRHHF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(12411..13442)
                     /gene="idnD"
                     /locus_tag="SI621_003100"
     CDS             complement(12411..13442)
                     /gene="idnD"
                     /locus_tag="SI621_003100"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HEI4065780.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGAEVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            13659..14222
                     /gene="idnK"
                     /locus_tag="SI621_003101"
     CDS             13659..14222
                     /gene="idnK"
                     /locus_tag="SI621_003101"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEI4065781.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(14226..15245)
                     /gene="ahr"
                     /locus_tag="SI621_003102"
     CDS             complement(14226..15245)
                     /gene="ahr"
                     /locus_tag="SI621_003102"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HEI4065782.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            15441..15525
                     /locus_tag="SI621_003103"
     tRNA            15441..15525
                     /locus_tag="SI621_003103"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15475..15477,aa:Leu,seq:caa)
     gene            15689..>16869
                     /locus_tag="SI621_003104"
     CDS             15689..>16869
                     /locus_tag="SI621_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEI4065783.1"
                     /translation="MKLTARQVETAKPKEKTYKMADGGGLYLEISAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARVKRDEAKKLLVQGIDPKAEQKEVQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKVRHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKRKEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMNLLDQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MV"
BASE COUNT         4033 a         4449 c         4238 g         4149 t
ORIGIN      
        1 ccggagcatg ctccggcctt cgttttcatc actgtgttga ttacagtgcg gcgataacag
       61 cctgctgttc aatcagcttg gctttcgctt ctgcataacc ttccagcttc tcacgctctt
      121 tcgcgataac cgcttccggt gcgcgggcga caaagccttc gttcgccagc ttgttctcga
      181 tgcggctgat ttcaccttcg atcttcgcca cttctttcgc caggcgcgcc agctcatctt
      241 ctttgttgat gaggccagcc atcgggatca gcagctctgc gccgtcgatg atcttggtaa
      301 cggaaaccgg acctttgtca tcggcaggca gcacggtgat gctttccaga cgcgccagcg
      361 tttgcaggaa gccacggttt tcgttcactc gacgttctgc atccgcgctg caaccacgca
      421 gcagcagttc cagcggtttg cccggcgcga tgttcatttc tgcacggatg ttacgtacag
      481 caacgatcgc ctgtttcagc cactcggtgt cggccagtgc cgcttcatcc acctgagacg
      541 catcgtactg cgggaacggc tgcagcatga tggtgtcggc agtgatacca caaagtactt
      601 tcacacgctg ccagatggtt tcggtgatga atggaatgat cggatgcgcg aggcgcagca
      661 gaccttccag cacggtcacc agcgtatggc gagtaccgcg cagttcggcc tcggtgccac
      721 cgttcattac cggcttagtc agctccagat accagtcaca gaactggttc caggtgaact
      781 catacagaat gcctgcggcg atatcgaagc ggaagctgtc cagcgcttcg cggtacgctt
      841 tgatggtctg gttgaactcc gccagaatcc agcgatccgc cagcgacagc gtcatttcgc
      901 cgccgttgaa gccgcaatcc tgaccttctg tgttcatcag cacaaagcgg ctggcgttcc
      961 acagcttgtt acagaagtta cggtaacctt ccaggcgctt catatcccag ttgatgtcac
     1021 gaccggtaga cgccagcgcc gccagggtga agcgcagggc atcggtgccg tgcggttcaa
     1081 tgccgttcgg gaactgtttc tcggtgcgct tacggatttt gtccgccagc tgcggctgca
     1141 tcatattgcc ggtacgtttt tccagcagtt ccggcagcga aataccgtca accatatcca
     1201 gcgggtcaat cacgttaccc ttggatttgg acatcttctg gccttcgtca tcacgaatca
     1261 gaccggtcat gtaaacagtg tggaacggca cctgcggttt gccattttca tctttgatga
     1321 agtgcatggt catcatgatc atgcgggcaa tccagaagaa gatgatgtcg aagccggata
     1381 ccatcacgct ggttgggtgg aactgacgca gggcgtcggt gttttccggc cagccgagcg
     1441 tggagaaggt ccataacgcg gaggagaacc aggtgtcgag aacgtcttcg tcctgacgca
     1501 gggcaacgtc ggtaccgagg ttattttctt tacgcacttc gtcttcgttg cggccaacat
     1561 aaacgttacc cgcttcgtcg taccatgccg ggatacggtg accccaccac agctgacgag
     1621 agatacacca gtcctgaata tcgcgcatcc aggagaagta catgttttcg tactgcttcg
     1681 gtacgaactg aatgtcgccg ttctcaaccg cttcaactgc cggtttcgcc aggacatcgg
     1741 cacgcacgta ccactggtcg gtcagcatcg gttcgataac cacgccgcca cggtcgccgt
     1801 aaggaacggt caggtcgtgc ggtttaattt cttccagcag gccgagtgca tcaaccgcag
     1861 caacgactgc tttacgtgca gcaaaacgct cgagtttctg gaactctgcc gggatttcgc
     1921 tggaataaac gtcagattcg ttacctttgg tatcgaacac ctgggcgctt tcacggatat
     1981 cgccgtcaaa ggtcaggatg ttgatcatcg gcagcgcgtg acgtttaccg acttcatagt
     2041 cgttaaagtc gtgcgccggg gtgattttca cgcagccggt gcctttttcc atgtcggcgt
     2101 gttcgtcgcc aacgatcgga atacgacggt taaccagcgg cagaatgaca tatttgccaa
     2161 tcagatcttt gtaacgcgga tcttccgggt taacggctac gccggtatcg cccagtaggg
     2221 tttccgggcg agtggtcgca accaccagat aatctttacc gtctgcggtt ttcgcgccgt
     2281 cagccagcgg atagcggatg tgccacatcg aacctttcga ttcgcgattt tccacttcca
     2341 ggtcagagat agcggtacgc agtttcgggt cccagtttac caggcgcttg ccacggtaaa
     2401 tcaggtcttc tttatacaga cgaacgaaaa cttctttcac cgcattggac aggccttcgt
     2461 ccatggtgaa gcgttcacgc tcccagtcga cggagttgcc gagacggcgc atctgacggg
     2521 taatggtgcc gccagattcc gctttccatt cccagatttt gtcgatgaaa gcttcgcggc
     2581 cgtagtcatg acgggtttta ccttcttctg cggcaatctt gcgctcaacg accatctggg
     2641 tagcgatccc ggcgtggtca gtaccgacct gccacagggt gtttttgccc tgcatgcgct
     2701 gatagcggat catggtatcc atgatggttt gctggaaggc gtgacccata tgcaaactgc
     2761 cggtgacgtt cggcggcggg atcatgatgc agaaactttc ctggctttca tcgccattag
     2821 gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata tcttgtgggt
     2881 tatatgtctt ttccattatt tccaggttgc cgtattcagg ttgaaaccag ccacgcggta
     2941 ggctttatag cgttcgcgcg ccagttgttt cagagaatct tcataaggaa cgaagtctac
     3001 cacttctgtg aaagcggtgg caaaatctgc aaagcttgtt cgcagactaa tcaatatatc
     3061 gcgcgggctg ctgctacgct tttgcggcca ggcgatctcc accggcgcac cgccgcgcgg
     3121 tccttctccc gctaaattat gcggaacaaa gctttctgcc ggacgcgccc acagggcttc
     3181 atccagccgg taagcctgct tttcatcttc acaggcgatg agcacgcgct taccgctgcg
     3241 ccaacgttct gcggcaattt cacacaccag ttgctcaacg gcgcttaagc catcgacggt
     3301 ggtgtcattg tccagaaggt agaacgttgc gtttttcata tatggggctt cttgtggtgg
     3361 atttaaatat tgctgcattc ctcaccctga aagatgagca atgaatttct attgcgctcg
     3421 atcggttccc tctcccttag ggagagggtt agggtgaggg taaaacgcaa acttactctt
     3481 cgccgttaaa cccagcgcgg tttaacagga actgtgccag caacgccacc gggcgaccgg
     3541 ttgcgccttt tgctttacca gaacgccagg cggtaccggc gatatccagg tgcgcccagt
     3601 tgtacttacg ggtaaagcgt gacaggaagc aacctgcggt aatcgcccca ccaggacgac
     3661 cgccaatgtt cgccatatcg gcaaaattgg actccagctg ttcctgatac tcgtcaccca
     3721 gcggtaagcg ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca
     3781 gcggattatg gttcgccatc agaccagtaa tatgatgacc cagcgcgatc acgcaggcac
     3841 cggtcagcgt cgccacatca atcaccgctt ccggctcaaa acgttcaacg taagttaaca
     3901 cgtcgcacag taccaggcgg ccttcggcat cggtgttcag cacttcaacg gtttgaccgg
     3961 acatggtggt taacacatcg cccggacgat aggcacgccc gccaggcatg ttttcacagc
     4021 ctgccagcac gccaataacg ttaatcggca gttgcagctc cgcgaccata cgcatcacgc
     4081 cgtaaaccgc cgccgcgccg cacatatcgt acttcatctc atccatgcct tctgaaggct
     4141 tgatggagat accgccggag tcgaaggtta aacctttacc caccaacacg attgggcgtg
     4201 catcttccga cgcgttgcct ttgtactcaa tcaccgacat cagtgattcg ttttgcgaac
     4261 cctgaccgac cgccagatag gaatgcatcc ccagctcttt catctgctgt tcgccgataa
     4321 ctcgggtgat gacattcttg ctgtagctgt cagccagctg gcgcgcctga gaggcgagat
     4381 aagcggcgtt acagatattc ggcggcatat taccgagatc tttcgctgct tttatcccgg
     4441 cggcaatcgc cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg
     4501 gcacgttgaa caccatttta cgcagcggac gacgcggttc gctcttattc gttttcaact
     4561 gatcgaaact gtagagcgtc tcttttgctg tctcgacagc ctgacgcact ttccagtagt
     4621 tgttacggcc tttaacgtgc agttcagtca gaaagcagac cgcttccatc gagccagtat
     4681 cattcagcgt attaatggtt ttctgaataa cctgcttgta ctgacgctca tccagctcac
     4741 gttctttgcc gcaaccaata aggaggatgc gttcagaaag cacgtttggc acatggtgca
     4801 gcaacagtgt ctgccccggt tttccttcca gttcgccccg acgtagcagg gcgctgatgt
     4861 acccatcgct gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga
     4921 cgcccacgac gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact
     4981 ccatgcacta cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag
     5041 ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc
     5101 attggcacgt cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac
     5161 gatttcgacg actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc
     5221 aaaagacgag tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag
     5281 acgctcaaaa gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag
     5341 ttagtgagga tcctcggcgc agcggttgac ggcgatattc cggcgaatct ggtgctctcc
     5401 cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc
     5461 gggctgctga tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc
     5521 tgcggcctga gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt attcacggca
     5581 atcgtcgcgg cggttaacgt gatgtgggcg ggaccgtggt catcgcgtca tcaggatgaa
     5641 gtgttagcag aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag
     5701 caagcgacta atggcagctc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa
     5761 gatgtgttcc tcgcgcaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc
     5821 gattccggac atttaaccca gctgcgcgac ggctcccagg tcgtcactct caaccaggga
     5881 acgcgcttcg aaggcactgc actgttacgt gatttccgca ttacggattt ccaggattat
     5941 caggcgatca ttggtcacca ggcggtggcg ctcgacccga acgataccga ccagatggac
     6001 atgcgcacat tgtggaacac tgacaccgat cgtgctcgcg cagaactgaa ctggcgtatc
     6061 acgttggtat tcaccgtgtt tatgatggca cttatggtcg taccgctgag cgtggttaac
     6121 ccacgtcagg gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc
     6181 ctgatccaga cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacgctgtgg
     6241 atgtggaccg ttaacctgat ttatctggct ttggcgattg ttctcaacct ttgggacacc
     6301 gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt
     6361 ttggcgtact tgaccgctat atcggtaaaa ctattttcac caccatcatg atgacgctgt
     6421 tcatgctggt gtcactgtcg ggcattatca agtttgtcga tcagctgaaa aaagccgggc
     6481 aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg
     6541 tgcaaatctt cttcccgatg gcggctctgc ttggggcgtt gcttggtctt gggatgctgg
     6601 cgcagcgcag cgaactggtg gtgatgcagg cttctggttt tacccgtatg caggtagcgc
     6661 tgtcggtgat gaaaaccgcc attccgctgg tcttgctgac gatggcgatt ggcgaatggg
     6721 tcgcgccgca gggcgaacag atggcgcgta actaccgtgc gcaggcgatg tacggcggct
     6781 cgttgctctc tactcagcaa ggcttatggg cgaaagatgg caacaacttc gtctacattg
     6841 agcgggttaa aggtgacgaa gagttaggtg gcatcagtat ttatgccttt aacgagaatc
     6901 gtcgtctgca atccgtacgc tatgccgcta ctgcgaagtt tgacccggaa cataaagtct
     6961 ggcgtctgtc gcaggttgat gaatctgatc tgaccaatcc gaaacagatc accggttcgc
     7021 agacggtgag cggcacctgg aaaaccaacc tcacgccaga caaactgggc gtggtggcgc
     7081 tggacccgga tgcactctcc atcagtggtt tgcacaacta tgtgaagtat ctgaagtcga
     7141 gcggtcagga tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat
     7201 ccgtggcggt gatgatgctg atggcgctgt cgtttatctt tggcccactg cgtagcgtac
     7261 cgatgggtgt gcgtgtggtc accggtatta gcttcggttt tgtcttctac gtactggacc
     7321 agatcttcgg cccgctgacg ttggtttatg gcatcccgcc gatcatcggc gcactgttgc
     7381 caagcgccag cttcttctta atcagcctgt ggctgttaat gagaaaatcg taaccgaatg
     7441 aacgaataaa gggagcgaaa gctcccttta ttattgttag caaagagtgc tttgttcatg
     7501 cctgaaaaat catgaaaatt caatatattg cagtagtgta ggcatgataa agcgcgccag
     7561 cgtcgcatca ggcaaagaca aatcgcacct gacttacctt cttctccccc ccagcaaact
     7621 ccccagcaca ccacgcacaa tctgattcgt cacctggcgg gcggcgcttt tcgccattgt
     7681 ttgcaccaca ccatctttct tcccgccgcg tggtccggta gtgccaaaca aaatatcctt
     7741 caatccaccg agaataccgt catccaccgc cacctctttc cctttcgcgg gaggattatt
     7801 ttgctgctcg gtactggcct gaaagccttt ttgcaacatc tcataggcgg attcccggtc
     7861 cacgtcatcc tcgtacttgc catacaccgg ggagtgatta atcaagccat tacgctcatc
     7921 ttccgtcacc ggccccattc gtgaacaggg ggcaatcacc atcgcccgtt ccaccactga
     7981 agggcttcct ttcgcatcga gaaacgagat taacgcctcg ccggtcccca gttcctggat
     8041 tgccttttcg gtatcaaacg ccggattggc ccgcatggtt tgcgccgcag cttttactgc
     8101 tttctgatct ttgggcgtaa aagcccgcaa agcgtgttga acgcgattac ctagctgtcc
     8161 gagcacatta tccggaatat ctgacgggtt ttgcgaaacg aaccagacgc ccacgccttt
     8221 cgagcggata agccttatca cctgctcaat cttatccagc agtacctgcg gtgcgtcatt
     8281 aaacagcaga tgcgcctcgt cgaagaaaaa caccagtttt ggtttctcca gatcgcccgc
     8341 ttccggcaat tgttcataca actctgaaag catccacagc aggctggcgg cgtacagttt
     8401 aggcatctga taaagctttt cggcgctgag gatattgata acgcctttac cgttagcatc
     8461 ggtgcgcatc cagtctttga tatccagcat cggctcgcca aagaaatgcg ccgcaccttg
     8521 ttgctccagc gacagtaatc cgcgctggat ggcaccaacc gatgcgctac tgatatttcc
     8581 gtactgattc tggaaggatt tggcgttatc gccgatgtac tgggtaatcg cccgcagatc
     8641 tttaaagtcg agcagtaaca gcccctgatc gtccgcaata cggaagatga tatttagcac
     8701 gccagactgc acatcgttga gattcaacag tcgcgccagc aacagcggcc ccagatccga
     8761 aaccgtcgcc cgcactgggt ggcctttctc gccaaagata tcccacacca ccaccggatt
     8821 ggcatgcggt tgccagtcgt tgacaccgat atttttaagc cttgcgagca gtttttccga
     8881 cgccgttcct gcctgcgcga tgccggtcag atcgcctttc acatcggcca taaacaccgg
     8941 aacaccgatt tccgacaatg actctgccag tttttgcagc gtaacggttt tacccgtccc
     9001 cgttgcgcca gtaatcagcc cgtgacgatt cgccattccc ggcaataaaa acagttctgt
     9061 atccgacgtg cgggcaatta acaggggttc gctcatgaga tttcctccag tttcctgcct
     9121 ggagtatagg caacgtccgg caaagagaat atgtgtaatg cagagaaaat aacctaaagc
     9181 gtgttaccgt gataaatctg atacccgagg tcaacagtgt tgtggttgtg atcgttattc
     9241 ttaattttgc tcagcaacat ttgggcggcc atccgaccaa tatcaaaacg gggagtaatt
     9301 acgctggcga ggcttgggat catttgcctg ccaatctcca gcccatgaaa acccgcaatg
     9361 gaaatttgtt ctggcacggc gaggtttcgc tcgcggcaca gcagaagcgc acccatcgca
     9421 atatcgtcat tggtgcaaaa tacaccgtct aaatccgggt ttgcgctaag ggcatcacgc
     9481 attaactgca ttcctaaatg aatggatgag atggcgcgtg gattcatgcg taatggggaa
     9541 agattatgca gcatcatcgc atcgcaatac ccctgataac gctgttcatc gcgggtgtca
     9601 tctttggaac cgagatacag aattttgcgc ctgactcgct tatccagcat ggtacacacc
     9661 atgtcaaaag cggcctgccg attatcaaaa ccgacctcca tatccagccg ttctccctgt
     9721 acatccatca attccacgac cggaatggtg gctgaacgca gaaatttcac cgtcctgatg
     9781 gtgtgatatt tttccgaaag tataatcccg tcaatgttat aagagagcag gttaatcacc
     9841 gactcttctt cggaatcgcg atcgtaattg taattagcaa taagggtctg ataattatgc
     9901 tcagaggtga cggattctat tcccgcgaga atgtcagcga agagttgatt ttgaaatgac
     9961 ggaatcaaga taccgagggt ataactttgt gcattcaaca acatgccggg agcgcgatta
    10021 gggatataat tgatctcttc cataattttg gcgatgcgct cgcctgtttc ctttgccacc
    10081 tttttcggcg agcggatata acgactcacg gtcatttttg ttacgccagc cagcgtagcg
    10141 atatcctgta aagaaattct gtgattgcgc ataacttctc ccaacgtcgg gggaatcttc
    10201 aatcttcccc cgaaactaca tcacaatatt ttattctttc agtgcaatac ggcgttaatg
    10261 gctaacacgc ccagcaaacc cataatagaa ataagggttt ccatcaccgt ccaggtacgc
    10321 aacgtttcac caaccgtcag attaaaatac cctttaaata gccagaagcc agggtcgttt
    10381 acgtgtgatg cgatcacgct gcccgcaccg gttgccagta ccattaaagc gggagcggca
    10441 tgggtaacgt tgataatcgg caacaccaca cccgcggtgg taatggccgc gacggtggca
    10501 gagcccagag cgatacgcaa cagcgccgca acagtccagc acatcaataa cggcgaaagt
    10561 gtagttccgg tcattaagtg tgaaatatac tgcccgacac cgctatctac taatacctgc
    10621 ttaaacgcgc cgccgccagc gataataaac acaatcatcg cgatagcgcc tatagaatcc
    10681 ccaatgatat ccatgatttg ctcgatggtg cgtccattac gtcggcccag tgtgaaaatc
    10741 gcaataacaa tggcaataaa cagcgcaacg gcagggttac cgacaaattc aaagaagagg
    10801 cgcacggtgt tagttttcgg taacgtaatt tcacaaacgg cggcgatagc catcaggatg
    10861 accgggatca ctgcagcaaa aatactgttc cagaaggagg gcatctcctc ttcgctaaac
    10921 agatgaggat taaataagcc ttccggtggt gctttctcaa agcgagttag cagtttagaa
    10981 aacagcggtc ctgcgacaat aactgtcgga atggtaatga taaatccata cagtaaagtc
    11041 gttccgagat tagcctcaaa gatagtcgcg atggcagtag gccctggatg tggcggcaga
    11101 aaacagtggg ttacagagag cgctgctacc attggtacgc caacatacaa caggggtaat
    11161 cctgatgatg ctacgatggt aaataccaac ggcaacagca ggacaaaacc cacttcaaaa
    11221 aacatggcga ggcccacaac cagaccggtg atcactagcg cccattgcac gcgtttttta
    11281 ccaaaagtag caatcagcgt agtggcgata cgttgtgccg cacccgtatc agaaatcagc
    11341 ttgcctaaca tggccccgaa accgaggatc attgccagcc cgccgagcgt gctgccgata
    11401 ccattttgta tagagtgcag gacggcctgt gcatccatcc cttcggcaaa tccgacgacg
    11461 gcagctacca gaacgagggc aataaagccg ttaactttaa agccgatcat caggataaga
    11521 agcagcgcga cgcctgccgc aataatgatt aatggcatag tctttatctc ttattagtgc
    11581 cagaaaagca gcgctttcgc gctgcacgta gggtggtggc tgttaaacag ccactaacat
    11641 gccgccatca acaaacaaca ggtggccgtt tacgaaatca gaggcttttg aagaaaggaa
    11701 caccgcagca ccaatcagtt cctgcggatc tccccagcgt gcggcggggg tccgtttgca
    11761 caaccaggcg gtgaaggctt cgtcctcaac cagtgcttta gtcatttctg ttttgaaata
    11821 gcccggcgca ataccgttga cctgaatatt gtggcgcgcc agctcgacgc acatgccgcg
    11881 ggtgagcatt tttaccgccc ctttcgatgc ggcataaggg gtgatggtgt cacgtcccag
    11941 ttcgctttgc atcgagcaaa tattaataac tttacctgcc ttgcgttcaa ccatgtgacg
    12001 agttaccgct tgcgatacca ggaacacggc ggtctggttt actgcgatca catcattcca
    12061 ctcttgttca gggaactcag taaaatgatg acggcgctgg ataccggcgt tattcaccag
    12121 cacatcaatg gggccgatgt tcttttcgat atgttcaacg gcagcatcaa tttcatgttt
    12181 atgagtaaca ttaaaaggtg cggcaacggc ctgaataccc tcctggtgga gtttttctac
    12241 agcaagttct gcgcgttcgg cagtaatatc attaataatt atttgtgcgc catatttacc
    12301 caggccggtt gccagtaaaa agccaatgcc ctgtgctgaa ccggtaatca agatattttt
    12361 tcctgccagt gaaaatagat cgttcattgt attattcctt tatttatctc ttagaaaaca
    12421 agctggactt ttgctgcctg ggttttatca ccggcgaaac gtagcgcctc ttccaggtca
    12481 gtgaagggat attcagcact cagtaaaggc agtggattga taacgccatt cgccagccat
    12541 gacactgcgg tattaaattc gctggtaaaa cggaaagagc ctttgagtga aatctcctta
    12601 ccaatcaatg tcatcattgg gaattctgcc atcgcgcctc ccatacctac ctgcaccatt
    12661 acgccgcgtg cacgagtgac ctccagacag gtattcactg atgaaggatg accggacact
    12721 tcaaagctga catcgaaata gcctttttcc gctttccagt gatccatgtc gtcgttttgt
    12781 gggtttacga gcacctccgc ccccatctct ttgcccagcg aaagggaacg gggactcaca
    12841 tcggcacaga caatttccgc ggcccccagt gttttcacag cactgacaat caggcagcca
    12901 atgggcccaa caccagaaat aaatactcgc ttgccctgta actcgccggc ctgatgtgcg
    12961 gcatgaatcg cgacggctaa aggttcggca aaagccataa ccttttcgtc agctttggcc
    13021 ggataaggga cacattgcga cgtttcgacc attttataac gggtaaaacc accatcaaca
    13081 tgagggaaat acatggcact gccaaaaaaa cgcatatctg tacactgatt ctcgttatgt
    13141 tcaatgcagt atttgcagtg accgcacggt ttagacggat taatggctac cgtttgccct
    13201 tcatgtaatt ctgatgagtc gctatgaata actttaccga taacttcatg acctaacacc
    13261 atcggtgcct ttatcatgaa attacctact tttccttcct gataataatg taaatcggaa
    13321 ccgcaaattc cacctcgggt tatttgtact aatgttccat tattattcca atctatcgtc
    13381 tgctcggtaa cggcaacagt tttcttgccc gcaacaacgc aggactgtgt tttcacttgc
    13441 ataaattcct cactggtcag gtagacacct cggaagcatt taagcggttt taattgtcat
    13501 tatttgtgat gaagatcacg tcagaaaatt gttacattac tatgttacgc ataacgtgat
    13561 gtgccttgta attcttatca gcagaaataa aaaaacgtga aattattatg ccaccaggcg
    13621 tagtatcgca gcaggtaaga tgattcagga gattttaaat ggcgggtgaa agctttattt
    13681 tgatgggcgt ttcagggagt ggtaaaacat taattggtag caaggttgcc gcgttattat
    13741 ctgctaaatt tattgatggt gacgatcttc atccagccaa aaatatagat aaaatgtcgc
    13801 agggtattcc attatctgat gaagatcgac ttccctggct ggaacgctta aatgatgctt
    13861 catacagtct ttataaaaag aatgaaacag gatttattgt ctgttcatca ttaaaaaaac
    13921 agtatcgtga tattttacgc aagggtagcc cccatgttca tttcctctgg ttagatggcg
    13981 actatgaaac tatcctcgcg cgaatgcagc gtcgggctgg gcattttatg ccggtagcgt
    14041 tactaaaaag tcagtttgag gcgctggagc gtccacaagc agatgaacag gatattgtgc
    14101 gcattgatat caaccatgat attgccaatg tcaccgaaca atgtcggcag gctgtgctgg
    14161 cgatacgaca aaacagaata tgtgcgaaag agggcagcgc ctcagatcag cgctgcgaat
    14221 gattttcaaa aatcggcttt caacaccacg cggtaacgcg ccttaccgtc gcgcacatgc
    14281 tggatggcgt cgttaatttt cgacatcggg aacagttcgg tagtcggcgc aaccttgctg
    14341 cgggcggcaa agcgcatcag cttacgcaac tcataaggtg taccggtagc agagccagag
    14401 acactgcgat cgcccgcaat cagcgtaaag gccggaaccg gcaacggcgt gagaaccgca
    14461 ccgacggtgt ggaaattacc gccatacgtc agcgcctcaa aataaggctg ccagtcgagg
    14521 ctgacgttga cggtgttgat aatgagatca aactgccccg ccagtgcttt cagtgcctgt
    14581 ggatcgcggc tattcaccac tttatcggca cccatcgcca gcacttcctg ctctttcgcc
    14641 ggattagaac taaaggctgt cacctcgcat cccattgcgt gcagaagttt tatagctata
    14701 tgccccagcc cgccaatacc aattacccca acgcggctgg tggcagtgat atggtgcatc
    14761 aacagcggtt taaagaccgt gataccgccg cacaacagcg gcccggcgga ctcgatatca
    14821 atattttctg gcagtggaat cacccattgc cagtccgcac gcaacttctc ggcaaagcca
    14881 ccgcgattca taatcgtcgg caccgcacct tgctcgcagt tgatctgatt accgctaata
    14941 caggcatcgc aatgcccgca gctgcgtgcc gtccagccaa tcccgacacg ctgaccgacc
    15001 tgcaaacctt tatcctgcgc ggcactcccg agcgccacta cgcgaccaat cacctcatgc
    15061 ccggcaacca gtggatattg tgaaaatccc cattcgttat cgatcattga cagatcggaa
    15121 tgacagatcc cgcagtaatc cacctgcact tcaacatctt gtggcttcag ttcaccggga
    15181 tcgtactcat aaacttccag ttcgccgccc gcttcttttg cggcatagct ttttatcatc
    15241 gacatttttt ggtccttctc tggtgttgtc tgggaagtgt agagcatggc agggcggggt
    15301 gcctggagtg tgacaaaggt tacacatcgc tgtatgcaat gctgaaaatt tcagcactta
    15361 gcgaggtgcg agcaagctgg cgcttgcatg gtggcgtgcg acaggtataa tccacaacgt
    15421 tttccgcata cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt gattcaaaat
    15481 caaccgtaga aatacgtgcc ggttcgagtc cggccttcgg caccattagt acttccaaga
    15541 ccatccgaga aagtccattt atccctttaa aatcaatgct tgaatcgatt tttaagtcct
    15601 gagtcgtccg agatagtccg ttgaaatccg gatgccattg ggggcataat tgggggcatc
    15661 ttaacttcga ttagaattgt gcccccaaat gaagctcaca gccagacagg tcgagaccgc
    15721 aaagccaaaa gaaaaaacct acaaaatggc cgacggcggc ggtttgtatc tcgagatttc
    15781 ggctaagggc tctaaatact ggcgcatgaa atacagacgc ccctcagaca aaaaagagga
    15841 tcgcctcgct tttggtgttt ggcctactgt gacgctagct caggcaagag taaagcgcga
    15901 tgaggctaaa aagctgctag tgcagggcat tgacccaaaa gccgaacaga aagaagttca
    15961 ggccgagaat tcgggggcat atacattcga aaccattgct cgtgaatggc acgccagtaa
    16021 caagcgctgg agtgaagacc atcgatcacg cgttcttcgc tatcttgagc tttatatatt
    16081 ccctcacatt ggttcgtccg acattcgtca gctcaagact agccacctat tagccccgat
    16141 taaaaaagtt gatgcaagtg gtaagcatga cgtcgcgcag cgtcttcagc agcgtgtcac
    16201 ggctatcatg cgttatgcag tacagaacga ttacatcgac tcaaatccag ccagtgatat
    16261 ggctggtgct ctatctacaa ccaaggtgcg acattacccc gctttaccct ctagccgatt
    16321 ccctgagttt cttgcacgtc ttgctgcata tcgtggccgt gtaatgacac ggatcgcggt
    16381 tgagctttcc ttactaactt ttgtgcgttc cagtgagttg cgtttcgcgc gttgggatga
    16441 attcgacttc gataaatctc tatggcgcat acctgcaaaa cgaaaagaaa ttaagggtgt
    16501 gcgttattcg tatcgtggta tgaagatgaa agaggagcat atcgttccgc tgagtcgtca
    16561 ggcgatgaat ttgttagacc agttaaagca gattagtggt gataaagagc tgcttttccc
    16621 gggggatcat gacgcaacta aggttatgag tgaaaacaca gtgaatagcg cattgcgtgc
    16681 gatgggatat gacacaaaaa ccgaagtctg cgggcatggg tttaggacga tggcacgtgg
    16741 tgcgctgggt gagtcagggt tatggagtga tgatgctata gaacgccaac tgagccattc
    16801 agaacgtaat aatgttcggg ctgcctacat tcacacttca gagcatttag atgagcgtag
    16861 gctgatggt
//
LOCUS       DARNVM010000105        16511 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.105, whole genome shotgun sequence.
ACCESSION   DARNVM010000105 DARNVM010000000
VERSION     DARNVM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.105"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..480)
                     /gene="yghD"
                     /locus_tag="SI621_003105"
     CDS             complement(<1..480)
                     /gene="yghD"
                     /locus_tag="SI621_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HEI4065784.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDVS
                     "
     gene            complement(482..1660)
                     /gene="gspL"
                     /locus_tag="SI621_003106"
     CDS             complement(482..1660)
                     /gene="gspL"
                     /locus_tag="SI621_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEI4065785.1"
                     /translation="MSSMLEIFFPLCASAPIRWQRRTADVECGIWPDVADECLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRAIVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFHAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKTASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1657..2634)
                     /gene="gspK"
                     /locus_tag="SI621_003107"
     CDS             complement(1657..2634)
                     /gene="gspK"
                     /locus_tag="SI621_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEI4065786.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLAFSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPIAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2631..3236)
                     /gene="gspJ"
                     /locus_tag="SI621_003108"
     CDS             complement(2631..3236)
                     /gene="gspJ"
                     /locus_tag="SI621_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEI4065787.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGPGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3233..3604)
                     /gene="gspI"
                     /locus_tag="SI621_003109"
     CDS             complement(3233..3604)
                     /gene="gspI"
                     /locus_tag="SI621_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEI4065788.1"
                     /translation="MKRGFALLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGNRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(3601..4164)
                     /gene="gspH"
                     /locus_tag="SI621_003110"
     CDS             complement(3601..4164)
                     /gene="gspH"
                     /locus_tag="SI621_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HEI4065789.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AVQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4168..4623)
                     /gene="gspG"
                     /locus_tag="SI621_003111"
     CDS             complement(4168..4623)
                     /gene="gspG"
                     /locus_tag="SI621_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEI4065790.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4640..5863)
                     /gene="gspF"
                     /locus_tag="SI621_003112"
     CDS             complement(4640..5863)
                     /gene="gspF"
                     /locus_tag="SI621_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEI4065791.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SYTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(5863..7356)
                     /gene="gspE"
                     /locus_tag="SI621_003113"
     CDS             complement(5863..7356)
                     /gene="gspE"
                     /locus_tag="SI621_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEI4065792.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYIRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7356..9416)
                     /gene="gspD"
                     /locus_tag="SI621_003114"
     CDS             complement(7356..9416)
                     /gene="gspD"
                     /locus_tag="SI621_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEI4065793.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9446..10405)
                     /gene="gspC"
                     /locus_tag="SI621_003115"
     CDS             complement(9446..10405)
                     /gene="gspC"
                     /locus_tag="SI621_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEI4065794.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRAM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVAVPVKQPEPAPVAETRLNVVLRGIAFGTRPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAISDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10423..10833)
                     /gene="gspS2"
                     /locus_tag="SI621_003116"
     CDS             complement(10423..10833)
                     /gene="gspS2"
                     /locus_tag="SI621_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HEI4065795.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(10899..11708)
                     /gene="pppA"
                     /locus_tag="SI621_003117"
     CDS             complement(10899..11708)
                     /gene="pppA"
                     /locus_tag="SI621_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HEI4065796.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(11848..16416)
                     /gene="sslE"
                     /locus_tag="SI621_003118"
     CDS             complement(11848..16416)
                     /gene="sslE"
                     /locus_tag="SI621_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HEI4065797.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRI
                     TGATCNGESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQ
                     ELAASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKK
                     LVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILS
                     EGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKST
                     IALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSN
                     GATLDEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTGGCNQARWFSLTTRNVNDGQI
                     QGVINKLWGVDTSYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDK
                     NYWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIG
                     NPHYNSILRCPNGYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKAS
                     MTVGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGF
                     EYVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESAS
                     GFVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDS
                     KTGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAK
                     IFEKFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQA
                     ADLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNKIEYRYENDKDDE
                     LGFKTFTEFLNCYANNAYSEGTQCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTG
                     LWAPAQKEVTIESTASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVT
                     FKVPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDSFV
                     YTAPKNNLNASNYKDKGGLKQFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHK
                     HRFTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVP
                     GATEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQ
                     LKEWAEKNFDIKTWYPDGNLPEFYSEREGMKGWNLFQLMHRKARGDEVGNNKFGSKNY
                     CAESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGATEMSFEGGVSQSAYN
                     TLASLDLPKPKQGPETINKVTEYSMPAE"
BASE COUNT         3652 a         4709 c         4179 g         3971 t
ORIGIN      
        1 actgacatca atttgtgtca cccgcagcgc atatttttca tccagtgcgt ttaaccagtt
       61 cagcagatca ttaaatacca caggatctat ccagacctgg atattctccc cgcgctcggc
      121 tattcgctta atgaccactg aatgcgccga agcactgtcg ctgatgaccc gcgatacctg
      181 tgctggcgtt gtcgtggcgg attttcgcgc cgcaataata tccggcgcag tgctcttcag
      241 tcgcgcgttc atcgccacga gctgctgcaa catcgtctcc tgttgttcta tccgttcgct
      301 caacggctgc cagatgagga cgtaatatcc ggcgctaaac aggaacacta ccgctgccag
      361 taacatgcct ttttcacgcg gagaacgtcc cgccaggtgt tgtgtcagcc agtgttcgcc
      421 acggcttaac tggcgttcac gccattgctg aaaatagtga ataaatttat cgcgtaacat
      481 gttatttcct ccgcaacgtt acgccgccgg aaaccgcatc accctctttc tgtaacgcgt
      541 cctgttgcac aacataatct gccgccagtg cactacgcag tttgtcgaaa ctggcaaagt
      601 tcgcggcccg cagctggagg tgaagcgtct ggcgtttttg atcaaaggtg aaaccacgca
      661 tctcgatgtc ggaaagtgac gccgttttca gagtactggc gatcgctgac aattctgcga
      721 gcagccgggt atcgtcggcc tgtgggcgat attttttcag cgccatcgtc acctgagagc
      781 gtaaattcac aatccgcttc tgctccggga atagcgttaa gaactgtttc tccgcctggg
      841 tgcggctttg cgccacctgt tcgctgacgc tccataacgt cacgccccgt tccactacca
      901 gcgcaaccag aatcagcaat atcggcagaa tcatcacccg ccagcgcgcc cactgttttc
      961 ggtagctgac acgaggctgc cacggccctg ttagcaggtt cccttccggt tcgccataag
     1021 tggtaatggc gggcagagcc gtaacggtca ggcgttcggc gtccgcacca gcccatgcag
     1081 acagttcttc cggtgcaatg ccgaccacgg ttagcgaaag cggtaaatcc tgctcattga
     1141 gctgggcgtg gaacatgacc ggagccagcg ccagcccggc actccatccc cggcattcat
     1201 cgatgcggca gataacccgt tgcgcatcgc cagccataaa cccacaagga atggacatcc
     1261 agtccggcgc gacgatagcg cgggtgatgc cgtttgcctg caaccactgc gcaatgttgc
     1321 gcatatgctg ctggtgaatc acagctacgg ttgccagttg ctggtcgatt ttcaacgggg
     1381 cgaaatgcag ttcatcgata tcctggttca gctcttcttc cagcaaggcg ggcagaatcg
     1441 tcggtatctg cttgcggggc acatcaggca gttcaacctg ccagacgctg atccattcgc
     1501 cgggaatgta gagtcgaatc gcatcagttt gcagccattg ctggagacat tcatcagcaa
     1561 cgtcaggcca gatgccgcac tccacgtcgg cggtacgacg ctgccaacgg atgggagcgg
     1621 aagcgcaaag cgggaaaaaa atctcaagca tggaactcac tcactttctc ctgtctgatg
     1681 ccagagaaca gaaaagtgtt gtgggcccat gcggacaatt aacgaattca tcgtcagttc
     1741 aatctcattc acggtgatat ctgaacgcag ccagaagtaa ttgctgtcca cgctcaggac
     1801 ggtttttagc tgttttttag tacgctcatc gacgtctgca agtagcggct gagcaagaaa
     1861 ctgatcgaca tcttcccagc ccttcgccgg acgttgttgt aataacgccc gcgcctgaac
     1921 agggcttaac cacgggtcaa acagcgcctc aagaatcaca ctttgcgtga catctaatgt
     1981 attgatgttg atttgctggc gggccatcgg cagcgcacag accagcggtt tcagtttttg
     2041 ataaagcccg gcgtccattc cctgtaccac gcgcatttcg ctgatatcag ccagcggttg
     2101 attagcggcg tagaacggca ccgagcgggc gagatactcg ctgtcttcac gccccagacg
     2161 cgtctgcacg ctgcggtcct cgtcaataaa ctcccacagg ctttcggcta tcagctcggc
     2221 ccgataagca ggcacatcca ggcgcgagat cagggcaatc agttgttgta ccgcgatagg
     2281 acgcgacgcc gttgtcggct gagcgagggc attcaggtta aagcaggcct gggcgtcacg
     2341 cagggtgacg gcaatttgcc cctgcggcag tgggaaaaaa cgcgggccag aagcccacgg
     2401 ctgtgccaga tgcacacgct tttcattttt caggctcaga ctgagtgcgc taaacgccag
     2461 gctttctgca ctggcgctgt accacagcgc ctgctggtac tcctgctggc tgcgcgttcg
     2521 cccaagttgt tgctgcattc gcccggaaag cgtgatggtc accagcatca taaccgccag
     2581 caataccagc accacgacca gtgccattcc gcgttttggt ggtgaggtga tcatgataat
     2641 tgcggcccgc gtaacaacca gatgcgttca atttcacccc attgcggcga atgcagggtt
     2701 atgcgtaccg ccacggggat cgcctgtact gacgaccagc tctcctgcca gcgcgtgccg
     2761 tcgtagaact gcaaacggag cgaatccgcc ggaatcaact tctgcattgt cggcttcacg
     2821 ctgcctgcgg catcggtcag cggccaggct aaccgttcga gataaccatc atgaatgcgg
     2881 taaccgacgg tgagcagatt actgcgtggc agacgcatca acgggttaac cacgccgcca
     2941 cgcacaaagc gcatcccttc actctcagac gccagcacgc cagggccagc cagtaacgct
     3001 ggttcacgct ggccctgatc gcctcttacc ggacgcggca tcatttgtgt cagatcgtgg
     3061 gtcagaaaac tcatcgtttg ctgcatgagg tttagttttt gatcgtgatc ggcgacggca
     3121 ctattcacgc gtgtgacgcc gtttgtcacc tgctgcgcca tcagtgccag tgaggcaaaa
     3181 atggcgattg ccaccagcat ttccagtaac gtgaaaccag cgcgagccct tctcactgtt
     3241 ggcctcccac ggcgctaaac caggcgcgtc gtgactgaat caccggcgaa aaatcttcgt
     3301 gacggctgac ttcaatatcc accgcctgga gcagcgcgtt acctgtattc agcggtatgg
     3361 tgcgccagta ccagcggttt ccagccatat cgctctcgcc ctgatgcctg accgcccgct
     3421 gctcgcgggt catcaggtag agcagcgcag tctggttttc agccacccag cctgctaccg
     3481 ttttctcctc aagaacgtgc tgattactca gcgcaccgct ggcaatctgt aacaccgccg
     3541 tggcagccag cgcaaaaatc gccagtgcga gcatcacttc cagcaaggca aatccgcgct
     3601 tcatggcatc ctctcatcac attgattgag ggataacgcg ccatcgtgtg ccagttttac
     3661 cgcccaacat gcgttttgca ccgctgagta gaagcgcagc gtaaacggcg tggcgggttc
     3721 aaaaggcgaa aaacggatct gcggcgtctt ctttttcgcc tctttttgca gctccagttc
     3781 aatatcgtgc agcgtcaggc gacgacgctg cagctccagc gcatattcct tctgccagat
     3841 atcactgccg ggttgtaaca gcatctgcac ctgtttcggc accgtaacct gtgccgataa
     3901 ccgggtcgca gaaacaggca accactgccc gtgacgacgc tgcataaact gatagcctgg
     3961 cgggtcgatt aacacaccga gtgtctgccc ttcgatcact gccctgtcct taaactgcgc
     4021 aaagcgagtc agaaaatcct gcgctgcttt tttcgcaggc ggttcagaat cggtcgcaaa
     4081 agtctgcacc acgcccgcac tggcaaggcc gataaggaaa atcaccagca tgatttccag
     4141 aagagtgaat ccgcgttcag gcactgatta ctgaaactcc tgcaaattcc aattaccgat
     4201 atctgcgcca gcgccttccc cattttcctg accatccgcc cccagggtgt aaacatcaaa
     4261 cagtcctttt tcacccgggc tgagatactg ataatcattg ccccacggat cttttggcag
     4321 tcgtttaatg tacccaccgg tacggtagtt acgggaatct gccatattgg ccggttgctg
     4381 gatcagcgcc tcaagcccct gttctgtggt cggataacgc ccgttatcca gtcggtacat
     4441 atccagcgca ttctccagcg ccacgatatc gctgatggct ttttgccgat ccgctttctc
     4501 tttattgccc aacaggttag gcaccaccag acttgccagg acgccaagaa taacaatcac
     4561 caccatcact tccagcaggg taaaacctgc ccgtggtttt tgtgtgcggg ataacgaatt
     4621 catgtgataa ctccgtaaat tacattccaa ccatattgtt cagttgcagc atcggctcga
     4681 ggatggcgat gacgataaac agcaccacgc ccgccatcat caccaccaga gcaggctcaa
     4741 acagccccaa cgccagcccc acctgggtat caaattctcg ttcctggttg actgcggcct
     4801 gttcaagcat ggtttccagt tcaccgctct gttcgccgga ggcgatcatg tacagcatca
     4861 tcggcgggaa caggcgcaga tccgccagcg cggcgcgcag actgcttcct tcgcggacgc
     4921 gatctgccgc cagcagcagt tgttgctcga catagcgatt tgccgacacg gcggcagcgg
     4981 tctgaatacc ttccagcagc ggaacactgc tggcggtgag gatgcttaag gtgcgggaaa
     5041 aacgcgccgt attcagcccg cgcgcaacac gccccgtcac gggcaaacgc agcaaggttt
     5101 tatcccagcg caggcgcatg gcaggatttt tgagtagccg ttgccccagc accagaagcc
     5161 ccagcaatcc cgccagccag taaacgccgc tggtctgtaa cgtgtagctc atggcgatga
     5221 gcatgcgggt ggaggcaggc agcgcgtgtc cgagatgatc aaactgttcg ataattttcg
     5281 gcactactgc cgtcagcaaa atagtgacca cgcccgttgc caccaccagc agtaccagcg
     5341 ggtagagcat ggcctgcagc aggcgtgatt tcagacgctg ccgctgttcg gtgtaatccg
     5401 ccaggcgatt aagcaccacg tcgagatgtc cggatttttc tccggcagcg accatcgaac
     5461 aaaacagaga atcaaagacg cggggatgtt cgcgcaggct gtccgacagg gtgtaacctt
     5521 cctgaatccg gctgcgtagc gccattccga ggctttttac atgcagtttt tcgctttgct
     5581 cactgaccgc ctgtaagcag gtttccagcg gcattgctgc ctgcaccagc gttgccagtt
     5641 ggcgcgtgaa cagcgccaga tctgccgccg ccacgcgacg atgtgcgtgc cgccgacgct
     5701 gcaacattcc ccctgcagaa gcattcatcc gggcttcaat gtgcacgggg ataagctctt
     5761 taccgcgcaa caactggcgg gcatgacgcg ctgaatccgc ctcaatcatg cctttggttt
     5821 tgcgaccatt acgctccagc gcctgatagt aaaacagtgc cattacgcct ccatggttac
     5881 ccgcagaact tcatcgagag aggtttctcc ggcgagcact ttctcaatgc cattgctgcg
     5941 gatgcccgca gagtgttgcc ggatataacg ttccagctcc agctccccgg cctgacggtg
     6001 gatcaaatca cgcaatgtgg cgtccaccac aatcagttca tggatggcag tacgtccgcg
     6061 aaaacctttg tgattacagg cggggcagcc ctgcggatgg tacagagtga cggcatgcgc
     6121 atcggtaatc cccagcaggc gtttttcttc gtcggtggca ggcgcggact ggcggcaatc
     6181 gggacacagc gtgcgaacca gtcgctgcgc catcacgccc gtcagactgg aagagagcag
     6241 gaaaggctca acgcccatat cctgcaaacg tgtgatcgcc cccaccgccg tgttggtatg
     6301 cagcgtggaa agtaccaggt gcccagtgag cgacgcctga acggcgattt ctgcggtttc
     6361 ggtatcgcgg atttcaccga ccatcaccac atccgggtcc tgacgcaaaa tcgcgcgcag
     6421 gccacgggca aaggtcatgc cgacgcgggt gttaacctgt gtctgaccga tcccttcaat
     6481 catgtattcg atggggtctt caaccgtgag aatgttacgc gaatggttgt tcagctcctg
     6541 caatccggcg tacagcgtgg tgcttttgcc ggaacccgtc ggccccgtca ccagaaagat
     6601 gccgtgcggt ttgtgcagta gctggcgcaa ctgcgcggtc agttcgtgac ttaaacccag
     6661 acgctccagc gtcaggcggg cctggttttt gtccagcagt cgcagcacca cgcgctcgcc
     6721 ccaggcggat ggcatggtgg agacacgcac gtcgatcgcc cgaccgccca gcagcagcgc
     6781 aatacggcca tcctgcggta cgcgcttttc ggcgatatcc agccgtgcca tcaccttaat
     6841 acgcgatacc agcagcgagg ccagtttgcg ccccggacgc agcatttcat gtaatgtgcc
     6901 gtcaacacga aaacggatca ccagactctt ttcaaacgtc tcgatgtgga tatccgaagc
     6961 gccttcttta atcgcctctg ccagcatggc gttgatcagt ttgatgatcg gcgcatcgtc
     7021 gtcactttcc agcagatctt ccgtttccgg cagctcttct gcgagggtaa agaagtcctc
     7081 ggcagaaccg agatcttcca tcagctggcg agcctcggaa gagtcccgct gccagacggc
     7141 attcagtcgc tgttcaaatt cggcctcgtc gatttgccgc agcgtaaagg gcgcgttcag
     7201 cccccgctgc agctcctgca aaacagagag cgacaacgga tgaacgtgga ggatctccag
     7261 cgacgcttcg caccatgcca ccaggctaaa ccgacggctg aaactgtagg gcagacgcac
     7321 ggtgttagcg gtggtttcct gtgctacagg caccattaac gcgttctccc ggcattgagg
     7381 aatgcgcgta cttccggcgg taaggcctga ttttgcgctg gcagtaccgg ctgcgcggtg
     7441 tgcggcatca ggcttaagcc ttgctcatcg cgatagatct gctcggcgcg catgtagtta
     7501 tatttgcgct gcgacacgcc gtctgccgcc ataccgtcac gcagaatggt cgggcggata
     7561 aacaccatca ggttacgttt ttctttttta tccgccgtcg atttaaacag gttaccaatc
     7621 aacgggatat cgcccagcag cggcactttc gccacgcttt ctcccgcctg gtcgtccatc
     7681 agaccgccaa gcacgatcag ttcaccatcg ttagccagca cggtggtctt cagtttgcgc
     7741 tcaccaaaca cgacgtcgag gctggtctgt ccttccacct tcgacacttc ctgctcaatc
     7801 accatctgta ccgcgttacc ttcgttaatc tgcggcgtaa ctttcagcat gatgccgact
     7861 tttttcctct ctacggtgtt gaaaggattg ctgttattgg agccaacggt agatccagtt
     7921 aataccggaa catcctggcc caccatgaag aaggcttcct ggttgtccag cgtggtgatg
     7981 ctcggcgtgg agagcacgtt cgagctggag tcgtttttga ccgcctgtac cagcgccatc
     8041 cagtcgcctt tcaccacgcc aaccgccgta ccgctaaagc cagaaagaag ctgagcgagc
     8101 gtggagagat cgccgttggt atccgggtta atggtggtgg caccgttttc gctgatcacc
     8161 gtggagcctt tctgcggttt cgcctgagaa atggctgcgc ccagcgtacc gatagggatc
     8221 tgcgtaccgt tagcaaactg cattaatccg gcatctttcg acgcccactg cacgccgaag
     8281 ttgatattgc tgccttcagc aacttccacg atcaacgcct cgacatgcac ctgagcacgg
     8341 cgaatatcca gttgttcaat cacgctttgc agcgactgca tgatgtcctg cggcgcggta
     8401 acaatcaggg cattactgtg tttgctggcg gcgatggaga caacctcacg cccgctaccg
     8461 accgtgcctt ccgcctcttc tttagccgcc gtgagcgtgc cgctgacctg cttcagtaca
     8521 tcgaccagat cttcggcttt gctgtatttg agatagaaaa cctggctgtt gccgctgcgc
     8581 tccatttctg agtccaggcg acggatcaga cggcgcattt tgtcccgcgt ggcggggtca
     8641 ccactgacaa tcacactgtt ggtgcgttca tcggcgacaa tttgagattt cagcgttgct
     8701 ggctggttct cgccgctgtt tttggtcagg ctttccagca cgcgggcgat ttccgaagcc
     8761 gaggcgttat ccagcgggat cacctcttca gtgcgattac ccgcgtgatc cacgcgctgg
     8821 atcacttctg tcaaccgctc cacgacggag gcgcgtccgg tgagcataat tacgttggag
     8881 ggatcgtaat taacaacgtt gcctgagcct gcgctgtcga tcatctggcg caaaatcggt
     8941 gccagttcgc ggaccgacac attacgtacc ggcactactt tggtgaccat ttcatcgccc
     9001 gcgtagttgt cgctgccttc accgaccaac ggcagtggct cgactttcgc ggcgctggat
     9061 ttcaccacct tcagcacgtc gttttccatc ggtacgacgg catacccctg tgcttccagc
     9121 aggttaagga atagctggta atactggcgt tcattgagcg gagtcatggt acgaatactc
     9181 actttcccct gtacgcccgg ccccataatg atggttttat taaggttagc gccgacggtt
     9241 tcgatgaacg atttcaggtc ggtatcttta aaattagcgg tgaaagtggc ttcttccgcc
     9301 cagaccggtg aactgcataa tgccgctgcc agcaccagcg gcagtaaacg cttttttgtt
     9361 ttgaggccag ttgtcttctt acgccagacc gacaacgtaa tatcacgcca aaacacgatg
     9421 aatgattctc ctggattaaa tgcggttagc gcagcgcgat ggaaatgtcg tggcgcgcac
     9481 ccttgcgtaa aaccgtaagt tgaatggaat ccattgaagg taactgccgc atcagagcaa
     9541 tcattgctcg tggatcagtg aaatcctgct gatttaacgc aatggcgata tcgccttcct
     9601 tgaaaccgct ggcatcgaac agagaacgat ctgcccccgg cttcactgca taaccgacaa
     9661 tcccctcctt acgcacaggc gtaagctgga tatagttaaa aattttctgc ggatctttcg
     9721 ccagtgcctg acgcacagca gtcgggatct caactggcgc actggcagca ggctcagcaa
     9781 cagcttgctt tgcttcgtca ctgatggctt ttttgttggt cacggctacg gtggaacgct
     9841 cctcttctgc caggctcagg cgctctattt ttccctgata gcgcagcatc acatggtcgc
     9901 ggttgatttc ctcaatcacc gcgttgtgag aaccaagccg ttccccctgc aaatagacct
     9961 gctgtttgcc gccttcttca ataaccgcgc caggtctggt accaaaggcg atcccgcgca
    10021 gcaccacatt aagacgtgtt tctgccacag gcgcaggttc aggttgtttt accggcacgg
    10081 cgacaggctg atatttgcca aaccagtttt gctggctgat taattgcacg tcatttttat
    10141 cgaacgtttt tgcatctgca cggggcggtt tattcgccga tggggaaacc gccgtatatt
    10201 ccgcagaaaa ggagaaatag cgccagagtg aatgcgccac ttttgcagaa ataataagca
    10261 gcatcagcca gaacatcgcg cgtacaattt ttcgcagatg ctctttgtct gtattaagag
    10321 attgtctctg gttaagagtg tgacgaattt ttgtcagcca ttgaatgaga taaattcgtg
    10381 cgtcacgaaa aacaacccgc gccaatttat atctacccga cgttatgctt tgactattcc
    10441 acaggtggta cgatccagtt tccgctgata ctggttacct gtacgtgtat cattaatcgt
    10501 tatgctgtaa ttaattccct cggtaatcat taatttcacc gtcgggtcag cgcacatttg
    10561 tcgttgatag ctggttaaaa aggcatcagg cgtctgcgtg gtctgagcac ccgcttcgct
    10621 gataatggtc atttttaccg tcgtgccgct actttgcgcc agcactaagg tatatcccgc
    10681 agatttaatc ggcaggtttt ggctgatatt ttgcgcctgt tttttcgcca gtaaactggc
    10741 attttcatta tgactggcac agccacttaa taatcctgta acgctcaaca atagcgatat
    10801 taataccctc cctggcattt gttttatcga catggtaaat aatctctaag gttattaata
    10861 agagtaaaaa tgtcacttcg ataatgacgt ggttatcatt aaaacaatgc ctgtagataa
    10921 agtgttgcta taccgcccag acttaaacac ggtccaaaag gcagtgtggt tgatcctctt
    10981 tttgtaataa cggcatatat caggccgcag catgaggcga ttaaagcaac gttgggcagt
    11041 gataacgccc ccacccaact acctaacgca gcgaacaata atacatcgcc catgcctaat
    11101 gcttctttac gcagaactat tccggctatc cagcgcagag agtaaaaagt gataaacccc
    11161 accaggacgc cggtaactgc atcttgtagt gttaacggac tctgttgcgc ccatgccgca
    11221 atcagcccgg tccacaatac gccctgagta aaaacatcgg gcagccattg gttgtcgagg
    11281 tcgatgacgc tcgcagcaat cagccaggcg gataatatca tcaccgccag cgcccatcca
    11341 ctttccggcc agaccagact cgccagcaaa aaagcgagtg ctgtcaataa ctcgaccagc
    11401 ggataacgct tgctgatttt cgcctgacaa tcacggcagc gccctttgag catcaaccag
    11461 gagagcagcg gaatattgtc acgaacgcgg atagtctgct ggcaatgcgg acagtgcgag
    11521 cgtggcagcg ccaggcttat ttttgactgc gtactcggca tttcaccgtg aaactccgcc
    11581 atttgttggc gcagcatgat ggggtaacgc caaatcacca cattcaaaaa actgccgata
    11641 atcaatcctc cgacggtagc caggacgggc atcgccgcgg ggtattgctg aaaaacatca
    11701 aaaaacatgg ttaaaggtta tttattgtaa cttgccggat gcgggcctgc aggtgtatgc
    11761 catacggctt tcctccaggc ccgataagct ggcgcaaccc gatgcgcctt atttcatgcc
    11821 ggatgcggcg cgaacgcctt atccgggtta ttcagcaggc atcgaatatt cggtaacctt
    11881 attaatggtt tccggccctt gcttcggttt cggcagatcg agtgacgcga gcgtgttgta
    11941 agccgactgg ctcacaccgc cttcgaagct catctccgtc gctcccggca actggtaagc
    12001 attcgcgccc ggattccatt tcttaaagaa ctccgaaaga tccgtctggg cgacccagga
    12061 ggcacacagc atcagcgtgt cggcagcgtt accgttagat tcagcacagt agttcttact
    12121 gccgaacttg ttattgccaa cctcatcgcc gcgcgcttta cggtgcatca actggaacag
    12181 gttccagcct ttcatccctt cacgctcgct gtagaactct ggcagattgc catctggata
    12241 ccacgtcttg atatcaaagt ttttctctgc ccactctttc agctgtgcgt acatcagcag
    12301 acggtccccc gcaccgccgc gcgcccatgc ctgaccgttg ctctcctcca gatattccgg
    12361 cgcgacggta atgtcgtcag cgacacggtt catcttgccg agatagcgat cctgcatgta
    12421 cagcgccagc acgttgttcg ctacttcagt tgcgccagga acagtcagcg gcgtttcggc
    12481 ggcgttgtga ccaacttcgt gccagatcag ccagtcgttc agcggcgtcg tcggcagcgt
    12541 ggtgctgttc gtcgagaagc tgctgttcat taccggataa ccagagtgcg catcaccgat
    12601 ggagatctgc acatcgttgg tgaaacgatg cttgtgcccc gtcaagtttt tataggtaaa
    12661 catccggtgc ttaccgtctt catcattacg accgtagaaa tcattcatcg agctggcaaa
    12721 ggtatccaga tctttagcga attgtttcag gccaccctta tccttgtaat tgctggcgtt
    12781 caggttgttc ttcggtgcag tgtagacgaa agaatctgac tccagctcac ccaacggcgc
    12841 aggggagttc agagcgtttt tccattcgcc gtctttatag aacggcgctt ttaccacacc
    12901 agtaaaggtg aattcggctg actcattctt tgggctgttg cccttgatat aaatcagacc
    12961 accgtaaggc accttgaagg tcaccttatc attggctttc agatcatagg ttttcgtcac
    13021 ttttggcgga cggttcagcg cgacttcatg cttctcgcgt ccggtcagat cgtcggccag
    13081 cgccacggtg acagtcacag gaactgatgc agtagactca atggtgacct ctttctgagc
    13141 cggagcccac aggccagtag actgcatgtt acccgcaaac catttggtcg gattcgagta
    13201 caggctgatg gtttcagtaa ccttctcacc ttctgccgat accgctcccg gatacttctc
    13261 gacatcaact ttgatgttca gatcccacca ggaacgaccc agcatcaggc gcgtcagcgg
    13321 tttttccata tagttgagcg gatagctcgg gttcatcatg cctgctttgc tgctaccgtc
    13381 accgtagatc atgttgttat cgacaagcga ttttttcaga tctgcagaac actgcgtgcc
    13441 ttcgctatag gcattgttgg cgtagcaatt caggaactca gtgaacgttt taaagcccag
    13501 ctcgtcatcc ttgtcgtttt catagcgata ttcaatttta ttccacagcc agaccgacat
    13561 gttctggtac agacgttcca gatcgacgct gctcagacgc tcacctttgc gaccattggt
    13621 ccggaagtag agctcatgct gatacagacg ctgaatgttg gtgcctaaat ccgcagcctg
    13681 caccatcgct tttgccgtgt cggcgttaag gcttagttgc gtatactgtg gaacatacat
    13741 gccaccagta accggaaccc ccgtgccagg acgatattcc agacagttga cctcgtagtg
    13801 gtaagttggg tccttacact cctttaatcc aggaaacttc tcaaagattt ttgccttcgc
    13861 agccttcaga gaatcctctg ttttatgatc ggcctcatca ataaaggcat aacgcgtttc
    13921 ctgtttgcca tctacatctt ccagccagct ggcaacttcc agcttcggtt tgtcatcagg
    13981 tttgttttct acctgatatt tccacttaac ttcccctgtc ttactatcga tggtgtacgg
    14041 cagcgcacca tctacggcag gataacgttc atagacccaa atgcccgttg cgcgctgctg
    14101 acgaacgcgg ttcggatacc cttgcggatc gttatttact accgacttgt tcagtgccat
    14161 cgacagacct gcggcatcaa gcagacgtac aaagccagac gcgctctctt ccttaagatt
    14221 gctcatcacg ttttccatga tcagcaccga tccacctttg ttcagatagg cgatcagatc
    14281 ggtcacatcc tgctgagtca gcttcggttt gctggtatct gctcgcagcg ggattgcata
    14341 aggatcgtta ccaacctgag tcacatactc aaagccgttg aggatcagca gcggcatttc
    14401 ctgcgggtcg agatcgccat agctacttaa atgctcaaca gagatacccg caaaatccgg
    14461 atgaaagccg aactcagcgc tatttcctgt aacctgacca tgacgtttga aatagacagt
    14521 atccaggttg gtgcctacgg tcatgctggc tttcgcgtcc ggagtccatt tatcgtcgga
    14581 cagatagcgc agtacgttct gcatgaagtg cttcatgtca tctgaatcac cgctgagcgt
    14641 acactgcccg tctttattaa cgcccccgtt ccagctgtaa ccgttcggac aacgcaaaat
    14701 gctgttgtag tgcgggttac cgataaccat cagtttgccc tcaccgactt gccccagcga
    14761 aataaacggc aggttaaagg tggcggtatc gcgcgtaacg ttttccggct caacaagaga
    14821 aggcgcttcc gtaatgtacg ccagctcgtt tttatcccag gcgcgtttct cgccgaaggc
    14881 cagccagtag tttttatcat tacgcgccat cagaatcggg aaggccgcgt tagagatatt
    14941 caccaccgcc tgaccgcgcg cattaccggt gctgccatag aagttggtgg agtcatggaa
    15001 tacatggaac ttgctgacag atttgtaact tgtatccacg ccccacagct tgttgataac
    15061 gccctgaatc tggccgtcat taacattgcg cgtcgtcagc gagaaccagc gtgcctggtt
    15121 gcaaccgcca gttttcgcac agattgcggt atcgatctct ttggcttgac ccgtcttaaa
    15181 ctgctcaata aattcgtttg gcagattaac aacttgctca ccttcatcca gcgtcgcacc
    15241 gttggataac gagagattga taatctcgtt gatcacgttg ggatattcgg caaagacctt
    15301 gcgtacatcg tccggaacaa cacgggtatt attttgcccg gtcgtcgaat agcgatgaat
    15361 aagctgatca atattcgccc cgcgaacttc atcacccagt tcagtcaatg caatggtcga
    15421 cttattgccg cgcactgagc ccagttcaaa ggtatcgata ccaaaggaga tggtttcgcc
    15481 ccagctaaag gaaaattcac cattttcccc tgtcacgcca cggcctgaat tggtgtagta
    15541 gttgacgcca gcaacaccat atccctggct atcgaccagt cggccttcgg agagaatgat
    15601 ttccgaaggt tgatactgat aaaactgttc cgcgttagcc gacacgaagg aagcgttcag
    15661 atccggtttt gttcccggcg tggtgacagg cacaaccggt gaggtatgag tggatggcgc
    15721 tttatcggtc gcagcattgt tttccacctc ttcattgacc agctttttga actcttccgg
    15781 tgccagatcg atttgcttat acagtgagtc gaaacgttta ctctcgatca cagaggagaa
    15841 cgtcaggcaa acctgttctg tattcgccgg acagctgtta ctggacgtta ccagcgaaac
    15901 cgcattgctt ttcttgtcat cggaagccgc cagttcttgc gcatcctcaa gactaaacga
    15961 cactttttca accgcacgca ggctacgcgc agcttctgac tgggtgtcga aggtagcaat
    16021 tgtcgtgttg ttccctgcca cacaggtgac tttgtcgcct ggcgtaaagg taaagccatc
    16081 gctggattca ccattacaag tagcaccagt tatccgctgg cttccgccca gggtcagata
    16141 acccgttttc gtaggaacag gttctggctc cggttctggt gttggctcag gatcaggtgt
    16201 cggttccggc gtaggttctg ggtccggcgt tggctcaggc gtcggctccg ggtttggtgt
    16261 tggatcaggt ttcacttccg gcaaagaccc tgttccagaa tctacaggcg gcgtatcgga
    16321 ggaagatccg gaaccaccgc catcacaacc ggctaacagg gttgcgctca aaatagccgc
    16381 taaaagcgat ttcttatatt taaatttctt attcattaat aacgcaagtg acaaaacaat
    16441 gttaaaaaat tcgtaactgg gagaaatagt tttatgcttt atcttctcta ataacttcct
    16501 ccattcttaa t
//
LOCUS       DARNVM010000106        16494 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.106, whole genome shotgun sequence.
ACCESSION   DARNVM010000106 DARNVM010000000
VERSION     DARNVM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.106"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(150..1574)
                     /gene="patD"
                     /locus_tag="SI621_003119"
     CDS             complement(150..1574)
                     /gene="patD"
                     /locus_tag="SI621_003119"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HEI4065798.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVLAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1596..2390)
                     /locus_tag="SI621_003120"
     CDS             complement(1596..2390)
                     /locus_tag="SI621_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4065799.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2380..3321)
                     /locus_tag="SI621_003121"
     CDS             complement(2380..3321)
                     /locus_tag="SI621_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4065800.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3322..4335)
                     /locus_tag="SI621_003122"
     CDS             complement(3322..4335)
                     /locus_tag="SI621_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4065801.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFHGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4353..5498)
                     /locus_tag="SI621_003123"
     CDS             complement(4353..5498)
                     /locus_tag="SI621_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEI4065802.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5743..7149)
                     /locus_tag="SI621_003124"
     CDS             complement(5743..7149)
                     /locus_tag="SI621_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEI4065803.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSNTATAMSVIENLPPGNVELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDHHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPAPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(7228..7644)
                     /gene="hicB"
                     /locus_tag="SI621_003125"
     CDS             complement(7228..7644)
                     /gene="hicB"
                     /locus_tag="SI621_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HEI4065804.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7690..7866)
                     /gene="hicA"
                     /locus_tag="SI621_003126"
     CDS             complement(7690..7866)
                     /gene="hicA"
                     /locus_tag="SI621_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HEI4065805.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8088..8318
                     /locus_tag="SI621_003127"
     CDS             8088..8318
                     /locus_tag="SI621_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HEI4065806.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8410..10371)
                     /gene="rlhA"
                     /locus_tag="SI621_003128"
     CDS             complement(8410..10371)
                     /gene="rlhA"
                     /locus_tag="SI621_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HEI4065807.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRTS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGHFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLAGY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10444..10980)
                     /gene="sutR"
                     /locus_tag="SI621_003129"
     CDS             complement(10444..10980)
                     /gene="sutR"
                     /locus_tag="SI621_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HEI4065808.1"
                     /translation="MENLARFLSTTLKQLRQHRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITLLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGKGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            11033..12244
                     /gene="ydcO"
                     /locus_tag="SI621_003130"
     CDS             11033..12244
                     /gene="ydcO"
                     /locus_tag="SI621_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HEI4065809.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTTQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     STLCGGMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(12279..12947)
                     /locus_tag="SI621_003131"
     CDS             complement(12279..12947)
                     /locus_tag="SI621_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEI4065810.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTNAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(13250..13843)
                     /gene="tehB"
                     /locus_tag="SI621_003132"
     CDS             complement(13250..13843)
                     /gene="tehB"
                     /locus_tag="SI621_003132"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HEI4065811.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFIIST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(13840..14832)
                     /gene="tehA"
                     /locus_tag="SI621_003133"
     CDS             complement(13840..14832)
                     /gene="tehA"
                     /locus_tag="SI621_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEI4065812.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            14956..15936
                     /locus_tag="SI621_003134"
     CDS             14956..15936
                     /locus_tag="SI621_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HEI4065813.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVCGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(15928..16467)
                     /gene="rimL"
                     /locus_tag="SI621_003135"
     CDS             complement(15928..16467)
                     /gene="rimL"
                     /locus_tag="SI621_003135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HEI4065814.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
BASE COUNT         4159 a         4290 c         3976 g         4069 t
ORIGIN      
        1 ggatgaaaaa tcaccaacat acatttagat cataataact gcctaatttt tccgctagtt
       61 attaatgtaa tcattctgat acttaatcac cattacactc agaacctaaa acgatagtgg
      121 ccgccctata acagccacta acatttaact taatgtttaa ccatgacgtg gcggacgacg
      181 gtgtaatcct ccagcccata aagtgacata tccttgccgt aaccagaaag tttctgccca
      241 ccgtgcggca tttcacttac cagcatgaaa tgggtattga cccaggtaca accatattgc
      301 agccgtgcgc tgacgcgatg cgccctgccc acatctttcg tccataccga agatgcaagt
      361 ccgtactggc tgtcattcgc ccagtttacc acctgttctt cgttgtcgaa gagtgtaaca
      421 ctcactactg gaccaaatac ctctttttgc acgatggcat cgtcctgtaa tgcgccagcc
      481 agcagcgtcg gcgcatagta atagccatta cccttgcgct tttcaccgcc agtgatcact
      541 ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc tgacgcgttc gagatgcgcc
      601 agcgagctta aaggtccaag ctccgtagac tcgtcatccg gcgcaccaga ttttaacgtt
      661 gccaccgcag cacccagttt ttccaccagc gtatcgtaaa tgcctttttg cgcgtagatc
      721 cgacaagccg ctgtacaatc ctgtccggca ttgtaatagc caaatgtacg tacaccttcg
      781 accactgctt caatatccgc atcatcaaaa acaatcactg gcgctttgcc accaagttcc
      841 atatgagtac gctttatgga cggcgcggta tggctgatga tgtgttcccc agtggcgata
      901 gagcccgtca gcgacaccat ccgcacttta ggatgtccgg tcagcggatc gcccaccgtt
      961 ttgcctctgc caaacagtac gttaatcacg cctgccggga agatatcttt cgccagctct
     1021 gccaacttca acgcggtcag cggggtaatt tctgatggtt taagcactac gcagtttcct
     1081 gccgccagcg ccggagccag tttccacgcg gccatcatca gcggataatt ccacggtgcg
     1141 atagaagcca cgacccccaa cggatcgcga cggatcatcg aagtatgacc ttcaagatat
     1201 tcacctgccg ccagaccatt cagacagcgc gccgcaccag cgaaaaagcg aaagacatca
     1261 acaatcgccg ggatttcatc attgaacgca ctatgcagcg gtttgccaca gttacgggac
     1321 tccagttcgg ctaaaacctg accattttct tcgataacat cagccagttt cagcaggcat
     1381 tccgcacgcg ctttcggcgt ggtttgcccc cattcggcaa atgctgcatc tgccgcgcga
     1441 acagcagcat ccacctgttc tgcggatgcc tcggcaatct ctagtaaaac gtcccccgtt
     1501 gccggattat agacaggctg tttttcccct tcgccgctaa ccagttctcc gttaatcagt
     1561 aacttatgtt gcatagcata ttcctgtatc agtggttatt gaccattgtc gccttcgcgg
     1621 gttagccacc aggcccccag gatcggcaag gttgttacca acataaccag cagtgccacc
     1681 acgttagtta ccggtacatc acgcggtcgc ccaagctgat tgagcaacca caacggtaac
     1741 gttcgttcat gacctgccgt aaaggtcgta acgatgattt catcgaacga caaggcaaac
     1801 gccagcattc ctcctgccag taacgccgaa ctgagattcg gcaacactac gtagcggaag
     1861 gtttgccagc cattggcccc aagatccatt gacgcctcaa ccagactcca cgaggtgcgg
     1921 cgaaaacggg cgatgacatt gttaaacacc accactacgc aaaaagtcgc atggccgacc
     1981 acgatggtga aaaatcccgg ctccagattg atggttttaa aggcggttaa tagcgccaga
     2041 ccagtgacaa tgcccggcag cgcaatgggc agcagcagta acagcgaaat ggcatttttg
     2101 ccgaaaaagt ctcgtcgcca cagcgcagca gctgccagcg tccctaacac cagcgccatt
     2161 aatgtcgcca gcgctgccac tttaagtgac agtgtcactg aatcaagaat atcactacgc
     2221 tgtgctgcca cgctaaacca gcgcagcgtc aggccttgcg gtggaaaact aaacgccgca
     2281 tcttcagtgt taaacgcata ggcggcgata atcaggatgg gaaaatgtag gaaaaccacg
     2341 ccgccccagg ccgccagttt gaggaaaaac ggtgcgcgtt cagagtgcat cgaacgctcc
     2401 cagacgtttc acgaacgcca ggtacagtgc gataagaaca atcggcacca gggtgaatgc
     2461 cgccgccatc ggcatattgc caatcgctcc ctgctgggaa taaaccatat tgccgataaa
     2521 atatcctggc gggccaacca gctgcgggac gataaaatcg cccagtgtga gtgagaaggt
     2581 aaagatagag ccagcggcaa tacccgggat cgccagcggc agcaccacat agcgaaaggt
     2641 ttgtcgtgga cgtgcgccga gatcagctga cgcctgcaac aatgacggcg gcaaacgctc
     2701 aagcgccgcc tgcacgggca ggatcatgaa cggcaaccag atatagagaa acaccagaaa
     2761 gcgccccagc ccggaagttg acagcgtatt gccgcccacc gcaggtaatg taaggaacgc
     2821 agtcagcagt ggttccagcc caagatgttg taaaaaccac tgagccacgc catcttttgc
     2881 cagcaataac gtccaggcat aggctttaac aatgtagctc gcccacatcg gcaacattac
     2941 cgcaatataa aaaaacgctt tcattttccc gctggtatag cgcgccatat accacgccat
     3001 tggaaaagcc agaatggcgc tggcgatagt gactgccaca gccatagtca gcgtgcggag
     3061 aatgatgtcg taattcgccg gattaaacag cgcacggata ttcgccagcg tcagttccgg
     3121 cgttaccgac atggtgaaat cgtcaaaggt ataaaatccc tgccataaca gtgtcagcag
     3181 cgagccgaaa tagacaatgc caaaccacat tagcgggcca agcagcagta aaaacagccc
     3241 cagccccgga ttacgccaga agaaaccgga caccttaccc agacctggac gtgaaggtga
     3301 ttgcaataca ttcatcgcca ttcacctctc ctcaaccagc ggcaccatca catcacgcga
     3361 ccaggaaacc atcacctgtt gtccgggcgt gagcgtggca ggcagttctt cgcctgtcat
     3421 attggcctga ctcacaagca gtttttcacc gccgttcaat ttcagttcaa aacgagttgc
     3481 cgcgccctga tattgcaccg cctggatcgt gccattggcc tgcagttcac caggggtgtt
     3541 gaggcggata tgttccggtc gcagggcgaa gcttcccgtc atgccacaaa gtttctctgc
     3601 catcagtcca tgaaaaacat tcgatgtacc aacgaacccg gcaacaaacg gcgtgcgcgg
     3661 gcgcatatag agatcacgcg gggaatcgac ctgctcaatg cgtccattat tgaaaaccgc
     3721 cacacgatcg gacatcgata acgcttcgcc ctgatcgtgg gtaacgaaga taaaagtgat
     3781 accgagagac tgttgcagct ttttcagttc cagttgcatc tgctcacgca atttgagatc
     3841 cagtgcgccg agcggttcat ccagcaataa cacacgcggt tcgttcacca gtgctctggc
     3901 gatggcgact cgctggcgct gaccaccaga aagttgtgac ggtttgcgtt gctgtacaaa
     3961 ccccaacgcc actttctcca gcgcctcttg cgccattgcg tgccgctgct ttttattcac
     4021 gcctttgacc atcagcccat aggcgacatt gtcaagaatc gacatatgcg gaaatagcgc
     4081 gtagtcctgg aagacagtat tcacgtcccg ctcccacggt ggcagattgc tggcgggttt
     4141 accaaagata gagatagccc cgccagaaag ctgttcgaag ccagcaatca ggcgcaggca
     4201 ggtggttttg ccggagccgg acggccccag catagagaag aactcaccat cttttatcgc
     4261 aatactgacg ccatctactg cgcgcacgtc accgtacaac cgcgagacgt tgtcaaactc
     4321 cactgcgtac gtcataaagc accccagcga agttagcgac cgcccataat ggcaatgtaa
     4381 tcctgcgtcc agcgactgta gggaacaaac ttgccccctt ctgctatagg cgttttccag
     4441 aaggcgattt tatcgaaata gttaaaaccg ttggtttcac aacctttttc gcctaataac
     4501 ggactggctt tacacccttc cggcactacc ggtaacgagc caaaccaggc cgccacatcg
     4561 ccctgcactt ttggcgttaa tgaccagttc atccatttgt aggcgcaaac cggatgtttc
     4621 gcttcactat gtagcatggt ggtgtcagcc caaccggtaa cgccctcttt cgggaatacg
     4681 gtcgcgacag gctggccttc ggctttcagg gcgttggcct gatagggcca tgcactggaa
     4741 gcaaccacac cctcgttttt gaaatcgctc atttgcacgg tagtgtcatg ccagtagcga
     4801 tggatcaaac tatgttgatc gcgcagcact ttcagcaccg cctggtactg ttcttcggtg
     4861 agttgatacg gatcgctgat gcccaactgc ggctgagtgg ctttaacgaa caacgcagcg
     4921 tccgcaatgt agatagggcc atcataagcc tggacgcggc ctttattgct cttgccgtcc
     4981 ggcagattct gctcaacaaa aaccacttgc cagctatccg gcggcgtcgg gaaggtttta
     5041 gtgttgtaca tcagcaggtt cggcccccat tggtaaggtg tgccgtaaac tttgccgcca
     5101 acgttaaacc agtcgccttt aaccacgcgc ggatcgagcg ctttccagtt gggaatcaag
     5161 gcagtattaa tcggctgcac gcgtttgccc ataatcaaac gcagcgaggc atcgccagat
     5221 gccgtaacca gatcgtaacc ccctttggtc atcagactga ccatttcatc ggaagtcgcg
     5281 gcggttttca cattcaccgc gcagcctgtc tctttttcga attgcgttac ccagtcgtat
     5341 tgtttatcag tttgtccgcg ttcgatgtat cccggccagg cgataatatc cagtcgccct
     5401 tccggtttat ctaaattggt aggcggttcg gcggcgtgag cggtcattat tgtcatggtg
     5461 agcgcacaca ggctgctgcg ggcaaatgtc ttgctcataa ggtcttactc ctgtcgtaat
     5521 taattgttca gccgccgtgc cgtaaaaata tctccttgtt taaccataga cggcggcttg
     5581 caggcacacc ttcgcccctg agaaaattta atcaggttgt gagctaccgc gcattatgcc
     5641 gtctgtaagg ttatcttctg gagtatagac aaggagttag gaaatatgac tgctattcga
     5701 aattcatatt acccaacctg agagaaattt aattaaacgc tattacagcc gttcttgaat
     5761 aagtttgcct aattgtttaa ccgcctgttc ttcacgttct ccccactgcc acgcggtatt
     5821 aaaacggaaa aaacgtgacc agttttcacc ggtagaaaac attttacccg gcgcaatact
     5881 gatatgatgc gtcagtgctg ccaggcttag ttcgcccgca tctaacggcg cggggagttc
     5941 cagccagaga aaataaccgc tgtcattatg atgaattttc acttccgcag gcagataacg
     6001 cagcagtgcc tgccaggcac gttgtttacg ctccgcaagc tggcgacgca agcgacgaag
     6061 atgggcgtcg tatcggcgcg tggaaaggta atccaccagc gcaagctgca tcggtgagct
     6121 ggtggaaagc gtactcatca actgcaagcg ttgaatttta cgtgcatgtt ttccggcggc
     6181 gacccaacca atacgaaaac caggcaccag acatttcgaa aacgaagagc aatgcaaaac
     6241 accatcgtgg tgatcccacg ctttcgcagg cagcggtttt tcccgtccaa aataaagttc
     6301 gctgtaaacg tcatcttcaa tcagcgttac gttgtactga ttgagcaacg ctaccagccg
     6361 cgcttttttc tgcggcgtta aggtaaatcc gagtggattc tggctattag tcatcagcca
     6421 gcacgctttc accggatact cctgcaacgc cagttccagc gccttgagat caatcccttc
     6481 tttaacgtcc gtcgccaccg ataacgcctt cagccgtaac cgctccagcg cctgcaacgc
     6541 gccgtagaaa caaggattct ctactatcac ccaatcgccc ggttcagtga ccgcttgtaa
     6601 actgaggttt aatgcctcta acgccccggc ggtaatgaca atttcatcag gagaaatagt
     6661 gatgccctgt aaggcatagc gacgagcaat agcctgacgc agttctacgt ttcctggcgg
     6721 taagttttca atcacgctca tcgccgtggc ggtattgctt acctgcgcca gcgagcggtt
     6781 tagttgttgg agggggaaaa gtcgcgggtc gggaaaggcc gaggcaaacg gaacgaccga
     6841 cggatcgcga ctagcctgca acatatcaaa aatataggtg ttgatatcga ctgcttcatc
     6901 tcgagtgaca ggaatgactg gcgctttcgg catttttatt gcctgtggcg caacgtaata
     6961 acccgactgc ggtcgagcga taatatgtcc ctgactttcg agcaactgat aggcatggct
     7021 gacagtcata aagctcatgc cagaaagcgc cacctggtca cgcaacgaag gcaaacgatc
     7081 gccaggttgc cagatacccg acgcaatctg ctcgcgtaat tgttctgcaa gctgctggta
     7141 ttttttcatt atcgaatcgt aattatgtgc agatgattcg gcagtctata tcagttgtta
     7201 aaaatgacaa cttttcgtta actgtaatta aaccatcacc agcgataact ctttgccaag
     7261 cgcctttgcc gcgagctgga cggcgtcgat ttttgtcgca tggtgcaaat taaataggcg
     7321 agtaatctcc tgtttaggtt tgccaattcg tctggctaac tcttgctgag tgatttctga
     7381 ctgtaaaaaa gcatttaaca gcaatacctt agaggcgacg ctcaaaggca cttcaataaa
     7441 gtgatcgtga ctatttaatg gcgaaggtaa cgggataagc tcgttatctt caaaataaaa
     7501 atcaaatgcg gtcagtaaag catcttttgc cgcttccatc gcttcagcaa ctgtttcgcc
     7561 ctgggtcaac gcttcaggga tatccacaaa agaaaccata taaccgcctt ccggcgcggg
     7621 tgtaagagtg acgggataac gcataatttc gactgtctct cgcatgtcat taaccttttt
     7681 aattggcgat taactcaaac cgagttgttt caggattgct ttacgcaatg gttctttaat
     7741 ctcatcgcag gggtgacgcg gcatgacact gcgcctcccg tgaaacctga gttttaaatg
     7801 gttgctgcca ttcgctacat cgacgccctg agattcgagc caacgtctga actcgctttg
     7861 tttcacatcg cctccctgcc gctgagtata tcaacaaagt aaacattagt gtttacctcc
     7921 gtgtgtaatg tgctcgaaaa tagcactaaa tcgccgcaac ggcagaatgc aatgcaaaat
     7981 caggaaggaa gattccagaa tttctttgcg ctaatttgca aaactgaata ataacctcaa
     8041 gaaacgatcg gcatcatagc ggcaaaaggt aagaaaaagg tggcaggatg tttacgaagg
     8101 cgttatcggt tgtcttatta acgtgtgcgc tgttctcagg acaactcatg gcagggcaca
     8161 aaggacatga atttatgtgg gtaaagaatg tggatcatca actgcgtcat gaagcggaca
     8221 gcgatgaatt gcgtgctgtg gcggaagagt cggcggaagg tttgcgcgag catttttact
     8281 ggcaaaaatc gcgcaaacca gaagcgggac aacgttgagt catttattgc cggatgcggc
     8341 gtgacgcctt acccggccaa caatatcatg cagattcaat aagttgtgag taaccagaaa
     8401 actggcgttt tacccttttc gcttcggcaa tgttttcagc agctcatccg gacttacgga
     8461 ggcgacaaca cttcccggta acggcatttt cagaatgtga tttttaattt tgccgatgac
     8521 gtgcatctca catgggcgac aatcgaactt tagcgttaat acttcatcgc cgttaaccag
     8581 ttgcattggc gtcgccttcc agcttttgat attgcctttc gcctgtttcg ggcacagatt
     8641 gaaggcaaag cgcagacaat gcttagtgat catcaccggg acttcgccct tctcttcatg
     8701 tgcctcatac gccgcgtcaa tcagctgcac accgtagcga tgataaaatt cacgcgcttt
     8761 ctggttgtat acgttcgcga ggaaactcag atgcgtttgc ggataaaccg gcgcaggatc
     8821 agcaaccggt ttacggctgc cccgctggta accggcaaga cgtgcagcat ccagcatgtc
     8881 ggcggcttca cggcggaact ggtttaacag actgtttggt acaaacagcg ccccgggcaa
     8941 attaatttgc acatcgcggg cgtaatagag ggtttgcccc agttttgcca gaccatcctt
     9001 tagattgttc attgcttttt cggcgttatt ggcttcgtcg aaatgcccgt ccagcgtatg
     9061 cgtgatgctg acaccctctt cactggtgag ggtcagaatc agttgttcct gccagccgcc
     9121 cagttcaata tctaccgcca cccgacgttc gctggatgtt tttgtcagtg cctgctgcca
     9181 gttatgatca agattacgat ttagtgggtg atgcgggcga attttgtgca actctgctgg
     9241 catttcattg ggccagacgc ggtactgatt ttctccggtt ttctcgaccg tattggcacg
     9301 aaagccgacg acttcacgtt taatcatcac gttcaggcca tcgccatttg ccagtggttc
     9361 ggtgacggca acatcgagat gatctttcgc cactttcaat acttcaccca ccggcaggcc
     9421 gataaatttc ggcgaatcga acgcgccaat atcgccttta cgggcattca caaaataatc
     9481 tgtgctacca cggtggaaag tcttttccgt cgatggaaca aagaaatgtt cagtacgacc
     9541 tgatgaagta cgcgtcagat cgccacgttc ttcaataatg gcatccagca tctggcgata
     9601 atgggcggtg atattcttca cgtagctcat atctttgtaa cgcccttcaa tcttgaagga
     9661 gcgtacgcca gcatcaatca gcgcgccgag gttggcagtc tgatcgttat ctttcatcga
     9721 cagcagatgt ttttcatagg aaaccacccg cccctgatcg tctttcaatg tgtatggcaa
     9781 acggcacgcc tgcgagcaat cgccacggtt ggcgctacgc cctgtttgcg catgagaaat
     9841 gtagcactga cccgaatagg ccacgcacag tgcgccatga ataaagaatt caatggtcgc
     9901 gtccgtggcc tggtgaatcg cgcggatctg ctcaagattc agctctcgcg ccagcacaat
     9961 ctgcgtgaag ccaacatcag agaggaactt cgctttttct actgtacgaa tgtcgcactg
    10021 cgtactggcg tgcagttcaa tcggcggaat atcaagttcc agaatcccca tatcctgaac
    10081 aatcagcgcg tcgacgccgg tctggtagag gtcggtaatc agccgttgcg caggttccag
    10141 ctcatcatca tgcaaaatgg tgttaagcgt gacgaaaatt tttgcaccat aacgatgggc
    10201 aaatggcacc agttcggcaa tatctttcag gctattactg gcattatgac gggcaccaaa
    10261 accagggccg ccgatataaa cagcatcggc accgtgcaaa atagcttcgc gggcaatggc
    10321 ggcatcgcgt gccggactta acagttcaag tcgatgagaa gatacggtca cattcttatc
    10381 cagtagctga aaaatggcgg ctattttagc cacaagcgcg acgttgcgaa atagttttcc
    10441 cgcttaactg cgcgggtaat ggatgaggga atgaaaatgt acgatttgct ccccaccatt
    10501 acgatagaca tgcgtaacgt ctgcagcaaa tcgagcgcct tttccacaat taaggctctg
    10561 ccattcacca tcaacgcaca gatcaagttg cccatcaatg acgacaacat gttcgattac
    10621 tcccttctca tggggcgtcg attcacttat tgcaccaggt gccatctgaa tcgagaagtg
    10681 ttcaaagcag agttgtggat cataaggaaa cagcaaagtg atcaccatcg cctgccgttg
    10741 tggatcataa actgaaggcg tagcggactg cggcggagaa ataaatgttg aaaatggcac
    10801 gttcaacccg gtggcaattt tccataatgt cgcgaccgtc gggctggatt cattacgctc
    10861 aatctgccct aacatcgctt tagaaacgcc cgtcgcttct gccagtcgcg aaagactcca
    10921 gccgcgatgc tgacgtagtt gtttaagtgt ggtggataaa aagcgagcga gattttccat
    10981 gcgactcctc cggcaaaacg gaagtttatc acttgtgcgt tataacggac aaatgctacg
    11041 gtacctgtac gttataacgc acgaggtgac tatgcgtctg ttttctattc ctccacccac
    11101 gctactggcg gggtttctgg cggtattaat tggctacgcc agttcagcgg caataatctg
    11161 gcaagcagcg attgtcgccg gagccaccac tacacaaatc tctggctgga tgacggcgct
    11221 ggggctggca atgggcgtca gtacgctgac tctgacatta tggtatcgcg tacctgttct
    11281 caccgcatgg tcaacgcctg gcgcggcttt gttggtcacc ggattacagg gactaacact
    11341 taacgaagcc atcggcgttt ttattgtcac caacgcgtta atagtcctct gcggcataac
    11401 gggactcttt gctcgtctga tgcgcattat tccgcactcg cttgcggcgg caatgcttgc
    11461 cgggatttta ttacgctttg gtttacaggc gtttgccagt ctggatggtc aatctacgtt
    11521 gtgtggaggt atgttgctgg tatggctggc aaccaaggcc gttgcgccgc gctatgcggt
    11581 aattgccgcg atgattattg gggtcgtgat cgttatcgcg caaggtgacg ttgtcacaac
    11641 tgatgttgtc tttaaacccg ttctccccac ttatattagc cctgattttt cgtttgctca
    11701 cagcctgagc gttgcactcc ccctttttct ggtgacgatg gcatcgcaaa acgcaccggg
    11761 tatcgcagca atgaaagccg ccggatattc agctcctgtt tcgccattaa ttgtatttac
    11821 tggattgctg gcactggttt tttccccttt cggcgtttat tccgtcggta ttgcggcaat
    11881 caccgcggct atttgccaaa gcccggaagc gcatccggat aaagatcaac gttggctggc
    11941 cgctgccgtt gcaggcattt tctatttgct cgcaggtctg tttggtagtg ccattaccgg
    12001 gatgatggct gccctgcccg taagttggat ccagatgctg gcaggtctgg cgctgttgag
    12061 taccattggc ggcagtttgt atcaggcgct gcataatgag cgtgagcgcg acgcggcggt
    12121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttttg
    12181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgcta acagaaaccg
    12241 ataataaaaa gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac
    12301 atggtggcat cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc
    12361 gtactttcgt tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta
    12421 ttagtcgctg catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg
    12481 cctgtagcca tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc
    12541 agcccttctt tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc
    12601 ccagcgttgg taaccagtgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta
    12661 tagttcaaaa ttttatccag aactttctgc cctacctggg ttgaaggttt cggtaccgga
    12721 taataagtga ttgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct
    12781 acccaacgta aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg
    12841 tttaaaaaac cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc
    12901 aacaagccgc ataaagctgc aactttcgca aatgatgtgg tacgcatgaa tagtttcctg
    12961 agatgattat attactttct aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa
    13021 aagtgttggt gaaaataaat tttttagccc cttttcagac gacatagtca atgccgaaaa
    13081 aggggctaag tcaatcatca gtagttaaat ttcagtaagt taagcggcta aaaaacttcg
    13141 actactgtga ttaatttcac atcccttcga taaaaaatat gcttaccgaa aagaagaaca
    13201 gtaaaccatc tcagcgtggg gaattttcac gcgctttgtt cttaccggat cattttttac
    13261 gtgccagcat cgtggcgaaa cgcagtttaa tacgattacc gttggcgtcg gtgcggtgca
    13321 gctcgccgac gtcttcattg tatttcacca tctcccagcc ttcgtaatat cgacgtaatt
    13381 ccccctcttt gaaggcaaac gggaagccga cggtacatgg ataatcagcg gtatccatcg
    13441 ccgccacaat caggttgtaa ccgccaggtt tagtgcaacg ttgcatattg gcaatcagcc
    13501 ctgggatggt tttagcctca aggaacatca gcaccacagt cgaaataata aaatcgtact
    13561 gtccatcaaa tgtgaggtta ttcagatcaa cgacccgggt gtgtaaatta tccagatttt
    13621 caatggactt aatgcgctcg acgttggcga tactcatggc atttttatcc catgcgtcaa
    13681 catcataacc attggctgcc aggtaaagac tgttacgacc attgccacag cccagatcca
    13741 gcgttttacc cggtttaacc actttcaccg cttccagcac ttcagagtgt gtgcgggtta
    13801 attcatattt atcagtaaaa tagttttcat cacgaatgat cattctttgt cctctgcttt
    13861 cattaaaacg gcgcgctcgg ttctgactaa caattttccc tgcatcagaa gcgcaaaagt
    13921 acggatgagc agtattgcaa taataaaatt ggtaaagata aacagcggca ccgccagcgt
    13981 atgaaaaaat ccattatcgc tgccactccc cagatgcaaa ccggtggttg ccagtgcaga
    14041 tacgccgaac gagaaactcc agaatgaagc attaaatggc tgggagagat accatggcat
    14101 cagacgtagc ataaacagca gttgcagcag tccataaccg aaaagcattt tcgccagcgt
    14161 gtcaccctcg ccgccgttga cgctcagcca ggcactacag gccaccagcg caggagcgag
    14221 ctgaatgccg agtgatgtcc gcagcgccgt gggtaattct cccgaactgc gcagacgctg
    14281 caagatcacc ggttcaaggc ttagccatga gaaaacgcct gcgcctaaaa acaccagacc
    14341 ggcgtcggtg tagcccaacg caccacaggc cattgcgctg ataaagttgt tggcaactgt
    14401 cggcagatac agtccaggcg tggtagcttc ttcagggtga gatccgcgcc ataatcccgc
    14461 agtttgccat gcggcataag ccagttgcac cacgacacca aaactgaaca ggcacaccgc
    14521 cagtgggcga aaccacggaa caaaaccaat cgccaccagc atcgtcgttg ccggaaacaa
    14581 actcacaaaa ctgctcagca ctggatggcg aacttccgcc agcacgctat gcggaaagcg
    14641 tatgagtcgg gtaataaatg cgctagtcaa taatccccag atgatcatcg ccagaatcac
    14701 cagcccatcc cctaaccagt ggctgacctg ccaaacctgg ctggcatagc gccaggcaaa
    14761 tcccatccct atcgtcccca acacaatacc aaagtagcct gccggcaaat tgagcacttt
    14821 atcgctctgc atcttcttac tcattttgtt taatttataa agtatatttg aaatgcattt
    14881 tatggaattt tctcgagcat agccagagcc gcagaatttg ctacggttat agtaagtcac
    14941 ctgtgggaaa acgctatgcg caaatatcgt ctaagtgaag aacagcgagc ctttagttat
    15001 caggaagatg gcactaaaaa aagtgtgtta ttacggcaga ttatcgccat tagcgatttt
    15061 aacgatgtga tagcaggaac cgctggcggc tggatcgatc gcgaaacggt gttagcacag
    15121 gaaggaaatt gctggattta cgaccagaat gctattgcgt tcggcggaac ggtgatatcc
    15181 gggaatacgc gtatcaccgg caccagcgtg ctgtggggag aggtttacgc aacggataat
    15241 gtctggatcg ataatagtga aatcagccag ggtgcatata taagtgacag cgtcaccatt
    15301 cgtgactcat tagtttgtgg tcaatgtaga atatttggtc acgccttaat tgatcaacat
    15361 tctatgatcg tcgccgcaca aggcttaacg cccgaccatc aactcctgct acaaatttat
    15421 gatcgcgcaa gggttagcgc ctcgcgtatt gttcaccagg cacaaattta tggcgatgcc
    15481 gtcgtccggt atgcttttat tgaacatcgc gccgaagttt ttgattttgc cagcgttgaa
    15541 ggtaatgaag agaacaacgt ctggctatgc gattgcgcta aagtctacgg tcacgcgcag
    15601 gtgaaagctg gcatagaaga agatgccatt ccgacgattc attacagttc gcaggtggcg
    15661 gaatatgcca ttgtggaagg taattgtgtg ttaaaacatc atgttttgat tggcggaaat
    15721 gccgtggtat gtggcggacc gattctgctc gacgagcacg ttgtcataca aggtgagagc
    15781 cgtattacag gtgcggtgat aattgaaaac catgtggagt taaccgacca cgccgtcgtt
    15841 gaagcctttg atggcgacac ggttcatgtc cgtggtccaa aagtcattaa cggcgaagag
    15901 cgcattacgc gaacgccact ggcagggtta ttgtgaatcg ataatacgcg cgtataagtt
    15961 cacatcatca taggcatcat tcaggaactc agcctgtttc aggcaacctt caaggataaa
    16021 accattgcgc aaagcgacct ggttgctttg cgcattgtcc acccgacatt tgatcacgaa
    16081 gcgtctaagt tcaccagact gggcgtaatg atgaatcaat gcctgcagcg cctgagaaat
    16141 gatcccctgc ccctgatgag attcgtccag ccagtagccg atttcagcgg ttttattcag
    16201 tggttcaata cgattaaacg agataacgcc gataagttca tcttctttga aaatcatgaa
    16261 cattttggca tatccgcgtt gatgcaacat tacattgccc tgcaccgttt ttcgcgtgtc
    16321 ctcttcactt tgaacaaact gcggccagtt tagcgactgc tgtaaccagg ttttattttt
    16381 acagattaac tgataaagag gtgtgacgtg attttctgca acagcatgta attcaagtga
    16441 ttcgcttact tttatcgttt cagtcatctt atctccagtc actccatgca gttg
//
LOCUS       DARNVM010000107        16470 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.107, whole genome shotgun sequence.
ACCESSION   DARNVM010000107 DARNVM010000000
VERSION     DARNVM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.107"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(34..852)
                     /gene="cof"
                     /locus_tag="SI621_003136"
     CDS             complement(34..852)
                     /gene="cof"
                     /locus_tag="SI621_003136"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HEI4065815.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGCVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            952..2652
                     /locus_tag="SI621_003137"
     CDS             952..2652
                     /locus_tag="SI621_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HEI4065816.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTV
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2717..3412
                     /gene="queC"
                     /locus_tag="SI621_003138"
     CDS             2717..3412
                     /gene="queC"
                     /locus_tag="SI621_003138"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEI4065817.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3464..3862)
                     /gene="fadM"
                     /locus_tag="SI621_003139"
     CDS             complement(3464..3862)
                     /gene="fadM"
                     /locus_tag="SI621_003139"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HEI4065818.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(3968..4339)
                     /locus_tag="SI621_003140"
     CDS             complement(3968..4339)
                     /locus_tag="SI621_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEI4065819.1"
                     /translation="MKHGIKALIITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4490..6361)
                     /gene="ppiD"
                     /locus_tag="SI621_003141"
     CDS             complement(4490..6361)
                     /gene="ppiD"
                     /locus_tag="SI621_003141"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEI4065820.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLE
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEEQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6553..6825)
                     /gene="hupB"
                     /locus_tag="SI621_003142"
     CDS             complement(6553..6825)
                     /gene="hupB"
                     /locus_tag="SI621_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HEI4065821.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7034..9388)
                     /gene="lon"
                     /locus_tag="SI621_003143"
     CDS             complement(7034..9388)
                     /gene="lon"
                     /locus_tag="SI621_003143"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEI4065822.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9576..10850)
                     /gene="clpX"
                     /locus_tag="SI621_003144"
     CDS             complement(9576..10850)
                     /gene="clpX"
                     /locus_tag="SI621_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HEI4065823.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(10976..11599)
                     /gene="clpP"
                     /locus_tag="SI621_003145"
     CDS             complement(10976..11599)
                     /gene="clpP"
                     /locus_tag="SI621_003145"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEI4065824.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11846..13144)
                     /gene="tig"
                     /locus_tag="SI621_003146"
     CDS             complement(11846..13144)
                     /gene="tig"
                     /locus_tag="SI621_003146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEI4065825.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13488..13805)
                     /gene="bolA"
                     /locus_tag="SI621_003147"
     CDS             complement(13488..13805)
                     /gene="bolA"
                     /locus_tag="SI621_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HEI4065826.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14110..14688
                     /locus_tag="SI621_003148"
     CDS             14110..14688
                     /locus_tag="SI621_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4065827.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTMTLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNIEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14732..16207
                     /gene="ampG"
                     /locus_tag="SI621_003149"
     CDS             14732..16207
                     /gene="ampG"
                     /locus_tag="SI621_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HEI4065828.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         3756 a         4429 c         3947 g         4338 t
ORIGIN      
        1 ccgggcaatt gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg
       61 atagtccagc cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac
      121 cggtaaatgc gggagctccg cgcgcaattg cggcatcgca ttgcccataa taaatccgct
      181 accgacgcag cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg
      241 caacgataaa cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac
      301 cggcagcact tcgaggcaat ccgtagcgga aaaacacaaa tgtgcacgct cgcctaatgc
      361 ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt
      421 ggtgacgctg ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata
      481 gacaaatgcc tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa
      541 gatatgcatg ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc
      601 aggtaaatca tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc
      661 ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg
      721 acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag
      781 ggttttctca cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc
      841 cagacgagcc atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg
      901 atatatacgg aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgactg
      961 ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt ccgccggaaa gccgcaaacc
     1021 gtcaccgtca gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg
     1081 ttacgccagg cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag
     1141 cgcgggcaac tacgctttct ggtcacgcca gaatcgctac gcaatgcgat gatggaacag
     1201 gcgctggaaa ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag
     1261 ctgcgcactc tgttacagcc gtttatgggc ggacaatggc aaaacgatac gcccacgttg
     1321 cgtattccct actatcgccc gctcgaaccg ctacaaccgg gctttttgcc cggccgtgcc
     1381 gagcagcatc tcgccggaca gatattttcc ggcctgaccc gcttcgataa taatacccag
     1441 cgcccgattg gcgatttagc gcatcactgg gaaacctctg ctgacgggtt acgctgggac
     1501 ttttatcttc gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta
     1561 caccagcgat tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg
     1621 aagcgtattg aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac
     1681 tggcttgcgc accggctggc gagctattgc agccatctgg cgcatccgca attccccctg
     1741 atcggcactg gtccttttcg cttaacacaa ttcacagtgg aactggtgcg cctggagagt
     1801 catgattatt accatttacg tcatccgctg cttaaagcgg ttgagtactg gataactccg
     1861 ccgcttttcg aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat cactatcggc
     1921 aaaccggagg agttgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc
     1981 tatttaacgt tgcgcaaaag tccccgactg tccctctggc aggcgcgaaa agtgatctcc
     2041 attattcatc aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc
     2101 agtcatgcat tactgccagg ctggactatt ccgcaatggc aagtaccgga tgaggtcaaa
     2161 ctaccgaaaa ccttgacact ggtttatcac ctaccgatag aacttcatac catggcagaa
     2221 cgtctacagg cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca
     2281 aaaaactggg acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta
     2341 attggcgaag caccggaata taccctggag caatggctgc gttgcgatcc actatggcca
     2401 catgttttcg acgctccagc atatgcacat ttgcaatcga cactggacgc ggtgcaagta
     2461 atgcctgatg aggaaaaccg atttaatgcc ctaaaagcgg tttttagcca gttaatggca
     2521 gacgcgacgc tgacgccgct attcaactat cactatcgca ttagtgcccc tcccggcgtg
     2581 aacggtgtgc gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg
     2641 ccgtcgcaat gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt
     2701 tcaagcagga ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac
     2761 cacctgtctg gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta
     2821 cggtcagcgg catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggggc
     2881 acgcgcgcat aaggtgctgg atgtcaccct gctcaacgag ctggcggtca gtagcctgac
     2941 gcgtgacagc attccggtgc ctgattatga acctgaagct gatggcatcc cgaatacgtt
     3001 tgtccctggg cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa
     3061 agcagaagcg gtgattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg
     3121 cgatgagttt gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg
     3181 ttttgaaacg ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta
     3241 cggcaaactg gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga
     3301 cggttgcggt cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc
     3361 cgataaaccg acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg
     3421 ggtgtcgccg gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct
     3481 cgagcttttc gcgcaattcc ccttccagag ctaacgcttt ctgcgtttta agatcaatac
     3541 aaacaaacgt aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct
     3601 ggcttaagat gccgctttta ccgtttaatt gctgcaactg actggtaatg gttaacaggt
     3661 cgcttaatac cgccgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt
     3721 tatgggccgt catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt
     3781 cgagaaactc cagatagcga gcgttgttta cgtgctggta aacgtcgaga tgatatccac
     3841 gaactttgat ttgtgtttgc atagcgcaat aaccttacgt tgtttttata aaaactgtta
     3901 taagtacaga ggccataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc
     3961 aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg cccatccctg
     4021 gcacctgctt gagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa
     4081 tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg
     4141 cattattaat gctgacccga gtgccttctt cgtcactggc tttcgccggt actgctgctt
     4201 tactttgtgc tgcaggagct tccgctttgg tttctaccgt tgtcggtttc gccgcaggag
     4261 ctgccgccag cgcgctatga gacattccgg cacaggccag agacagggta atgatcagtg
     4321 ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac
     4381 ggcagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac
     4441 cttttcttgt tacagctgca aagcattttt ttgcgaggcg gcttcaggat tattgctgtt
     4501 ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa
     4561 agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgttcttccg
     4621 gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg
     4681 tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc
     4741 taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag
     4801 cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca
     4861 ctttcgcctg ttgttcagct ttgttatgct gaaccagtgc cttaacttgt tcctgaacat
     4921 ctgccaacgg tttcaccgct tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt
     4981 cgccatcgac ggtgatgatg tcagagttga tgcccggcgc gccgttttca cctaccagac
     5041 cgccgttgaa gatagcgtca gcaaccggct tgaagttcaa ctcttccggc aggttatctt
     5101 tgctgaacca acccgtctga gtggctttaa cgccggctgc ttgctctgca ccggccagag
     5161 actcggtgtc gttgcttgcc gcatcgctca ctttctgttg cagtgcgtag tacgcatcga
     5221 gggctttttc gtgtttcact ttcgccgcga catcgtcacg cacttcgtct aacgacttca
     5281 ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga
     5341 caccagacag ttggcctttt tctttcaggc cagcattttt tagttcgtcc gggatagtgg
     5401 catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt
     5461 ctttggctaa cgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt
     5521 catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat
     5581 cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg
     5641 cggcatccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt
     5701 tttgttcgta gtagctggca atttcctgtt cggtcacagg ctgcttcgcc gccagcgcgt
     5761 taacatcgat agtcgcttca cgcaccacgc gttgttgagc taccagtgcc gccagctcgt
     5821 cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag
     5881 tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga
     5941 taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa
     6001 tcgcctgttt aacctgctca tcgctgatac ccagtttcag ctcacgagcg tactgctcca
     6061 gcagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt
     6121 cgttcgctgc aagctcagag tattgatcgc ccagctgttg ctgcatgcga ttacgctcgc
     6181 tgttgaaggc gttctcgaat tgcccacggc tgatttcctg gtcattcact tttgcggcgt
     6241 agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa
     6301 tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca
     6361 tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc
     6421 aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacgc agaaagtcac
     6481 cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac
     6541 tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac
     6601 tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc
     6661 acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag
     6721 agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat
     6781 atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt
     6841 tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc
     6901 agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgccatc taacttagcg
     6961 agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt
     7021 ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg
     7081 ccagagtcag aacttcctca atgcgtttca caggatgaat gtccagatcg gcaattacgt
     7141 tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattaacact gttttaatcc
     7201 cgccgcgatg cgctgccagt agtttttctt tcaaaccacc gataggcagt acctgaccac
     7261 gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag
     7321 aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt
     7381 ccggtacgtg gacgtggatg tcgcgttttt cgtaaaaatc agggttgatc cccagttttt
     7441 ccgcacgcgc acgaaccacc gttaacgccg cctgaatgga ctcctgcatc acttcaccga
     7501 gcgaaccggt ataggtcagt ttgcctttcc ccggaacaca tgcggtttca atggtcagca
     7561 agtcaccgcc cacttccgtc cacgccagac cggttacctg accgacacgg ttttcgttat
     7621 cagcgcgtcc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcaccgttaa
     7681 tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg cgacacagtt
     7741 tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtaataa cgaataatgc
     7801 cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct
     7861 gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga
     7921 ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg
     7981 cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta
     8041 cgttctgctc tggatccagc acttcaagca gtgcggaggc cggatcgcca cgcatgtcag
     8101 aagacatttt gtcgatctca tcaagcagga acagcgggtt tttcacgccc actttcgcca
     8161 ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt
     8221 ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg gttgctttgg
     8281 cgatggactg acccagggag gttttaccta cccccggcgg ccctaccagg cagaggatcg
     8341 gtcccttgat tttgttgaca cggctttgaa ccgcaagata ctcaaggatg cgatctttca
     8401 cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt
     8461 tgaccttgct acgcgcattc cacggcacct gtaccatcca gtcgatataa ccacgcacta
     8521 cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt
     8581 tctcttttgc ctctttcggc attttcgccg cgtcgatttt gcgcttcagg gcttcgtttt
     8641 cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca
     8701 gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct
     8761 caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt
     8821 taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag
     8881 caatggtatc cgccagacgc gccgggtcgt cgatgctatt cagcgacgtc agcacttctg
     8941 gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca
     9001 gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa
     9061 agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca
     9121 ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg
     9181 tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa
     9241 tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa
     9301 ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt
     9361 caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt
     9421 aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga
     9481 tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc
     9541 cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct
     9601 tccggcttgc cataaatcag caacggcttg ctttgaccat caattaccga ttcgtcgata
     9661 accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag cagtgcggct
     9721 tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata
     9781 gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga
     9841 tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc
     9901 agttcattca acgttgcgac aaccggcaga cgaccaataa actcagggat aagaccaaac
     9961 ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac
    10021 ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg atgggaaatc
    10081 actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc
    10141 tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gcggaacagc agctaccgta
    10201 ccttcgatca gtttcaacag tgcctgctgt acgccttcac cggaaacgtc tcgggtaatg
    10261 gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc
    10321 tgcgctttct gtacgtcgta atcgcacttc tgcaacagct tctgaatgat gttttcaacg
    10381 tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg
    10441 tccagcaggc gcgccagcgt ttcggccagc agcgttttac cggaaccggt cggaccgatc
    10501 agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt
    10561 ttgtagtggt tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg
    10621 taatcgtcca ggtggttacg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc
    10681 ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg
    10741 cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa
    10801 aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac
    10861 ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg
    10921 cttacagcgt tgtgccgccc tggataagta tagcggcaca gttgcgcctc tggcatcaat
    10981 tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga
    11041 agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca
    11101 taagttcatt catgcgtcct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg
    11161 tcgcctggcc ctggtagccg cccaacggct ggtgaatcat cacgcgcgaa ttcggcaggc
    11221 agaaacgttt accttttgcc cctgcagtca gcaagaaagc gcccatcgaa gccgcctggc
    11281 ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc
    11341 cggcagtaat caccccgcct ggggagttga tgtacagata gatatctttt tctgggtttt
    11401 ctgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc
    11461 cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac
    11521 cacgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc
    11581 gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaacct gctgtaccga
    11641 ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata
    11701 tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggtt
    11761 ccatgcaaaa ttcatgacaa aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg
    11821 aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg
    11881 tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag
    11941 caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt
    12001 tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag
    12061 ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcgacgtt
    12121 tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga
    12181 aacgctgtgc agcctgacga cgcagaacgt cgatttcact gtcgatcagc gcagccggta
    12241 cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga
    12301 tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg
    12361 aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct
    12421 cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt
    12481 ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga
    12541 taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg
    12601 ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt
    12661 cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat
    12721 ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg
    12781 cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag taggtgaagt
    12841 cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt
    12901 ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg tcctggcgta
    12961 cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt
    13021 caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt
    13081 cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt
    13141 gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt
    13201 tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc
    13261 atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga
    13321 acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg
    13381 ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa
    13441 atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt
    13501 cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta
    13561 atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta
    13621 ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc
    13681 acaactttaa aatggctttc agagccggct gggacattgt gacgatagct ttcatccact
    13741 acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc
    13801 atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg
    13861 cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttctg
    13921 tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct
    13981 tttaccaggg gcgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct
    14041 cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag
    14101 agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga
    14161 tgcgcaaaac cgccaacaac tattgaagtt tccccgacga tgacactgcc acagcaggat
    14221 ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg
    14281 ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgatctgcgt
    14341 ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggtcggtccg
    14401 aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgatgt gtcccagggc
    14461 aacgtgcgct ataacatcgc gaccaaagcg gatatcgcca tcatcgctac cgcgcagaat
    14521 ggcaacaaaa tgaccaagaa ctatcgtgcc agctacaaca ttgaaggtgc gttccaggcc
    14581 tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg
    14641 tctcaggaca ccagcatcca cgaattcatc aagcagaacg cgcgttaatt tctgccctct
    14701 ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca
    14761 acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct
    14821 gacatccggc accttacagg cctggatgac ggtcgagaat atcgacctca aaaccattgg
    14881 tttcttctcg ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga
    14941 ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct
    15001 gttattagtc gccattgcgg cgatgggttt tctcgaacct ggcacccaac tccgctggat
    15061 ggcggcactg gcagtggtga tcgctttttg ctctgcctcc caggatattg tcttcgatgc
    15121 gtggaaaacc gatgtgctcc cggcagaaga acgtggcgcg ggcgcggcaa tcagcgtgct
    15181 gggttaccgt ttagggatgc tggtttccgg cggcttagcc ctatggctgg cagataaatg
    15241 gctgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct gtattatcgc
    15301 gacgttgctt gcaccagaac caaccgacac cattcctgtg ccaaaaacgc tggaacaagc
    15361 ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct
    15421 tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat
    15481 tcgtggcgtc gggtttgatg cgggtgaagt aggcgtggtt aacaaaacgc ttggcttact
    15541 ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt cactgttccg
    15601 ggcgctgcta attttcggta ttttacaagg tgcgtctaac gccggttact ggctgctgtc
    15661 gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg
    15721 cgggatgggt acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc
    15781 cgctactcaa tttgccctgc tctcagcgct ttctgctgta gggcgagttt atgtcggccc
    15841 cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctgt tctccgtcgc
    15901 cgctgccgta ccagggctta ttttgctgct ggtttgccgc cagacgcttg aatatacacg
    15961 agtaaatgac aactttatct cccgtaccga atatcctgca ggttatgcct ttgccatgtg
    16021 gacactggcg gcgggcgtca gcctgttggc cgtgtggtta ctgctgttga cgatggacgc
    16081 gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc
    16141 cctttctggc gtcgtgcttg gtggtttact ggattatctg gcgctacgaa aaacgcatct
    16201 gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta ttacggctaa
    16261 ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg tctggaaatt
    16321 attgacgcca tttatgaatt ctatttttct tacacgattc agctaatgag tctttatttt
    16381 ctcactaccc agttgtcacc ttaatgataa ttatttgtta aataattgtt ttatttcgca
    16441 ttggttatac caattgcccg cccagactcc
//
LOCUS       DARNVM010000108        16375 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.108, whole genome shotgun sequence.
ACCESSION   DARNVM010000108 DARNVM010000000
VERSION     DARNVM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.108"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..205)
                     /gene="emrE"
                     /locus_tag="SI621_003150"
     CDS             complement(<1..205)
                     /gene="emrE"
                     /locus_tag="SI621_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HEI4065829.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGI"
     gene            complement(273..575)
                     /locus_tag="SI621_003151"
     CDS             complement(273..575)
                     /locus_tag="SI621_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HEI4065830.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRLLRVYKRTSMGTQ"
     gene            complement(572..1273)
                     /locus_tag="SI621_003152"
     CDS             complement(572..1273)
                     /locus_tag="SI621_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HEI4065831.1"
                     /translation="MKNIAAQMTNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1270..2289)
                     /locus_tag="SI621_003153"
     CDS             complement(1270..2289)
                     /locus_tag="SI621_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEI4065832.1"
                     /translation="MTGSKMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVEK
                     PDAAIQSGSRWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2286..2825)
                     /locus_tag="SI621_003154"
     CDS             complement(2286..2825)
                     /locus_tag="SI621_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HEI4065833.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAEDN
                     QDVVAALIVNEYRAQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2856..3083)
                     /locus_tag="SI621_003155"
     CDS             complement(2856..3083)
                     /locus_tag="SI621_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4065834.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            3194..3886
                     /locus_tag="SI621_003156"
     CDS             3194..3886
                     /locus_tag="SI621_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4065835.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPTVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            3953..4816
                     /locus_tag="SI621_003157"
     CDS             3953..4816
                     /locus_tag="SI621_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065836.1"
                     /translation="MDCDALQDIKISLRYDGKDALNHEIDLNCLGESLKGFSKVLSTA
                     ASFSVTQKYSKYINYQEVKVYAREAKANCFTLEAVLNFATQNQLFSGIAATILGAILQ
                     YIFARNSNKKDEMKALQQSLEKAIEALGNKDAGTIDKLISLIDRMAVELRPSVRQAVS
                     PIGNTCDQISVATNVDGCLLKVNERDKAEIDRLDDDEVLGLREYRAFLTEFDAQNMTA
                     KIILDGDDSKKRITAEISDPAAGKKNNPYIRALSAYISTKGDPSAVFTITAKATVKKG
                     QINRLFIVDAK"
     gene            5209..5499
                     /locus_tag="SI621_003158"
     CDS             5209..5499
                     /locus_tag="SI621_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065837.1"
                     /translation="MTTGGHPPPEYAQKSLYVQQPSLRAGVFMVHQHYGTQTVNRGAV
                     RPGMLVKHKDGTWTASANLRGRLYLHRGIERTYTKDLLVEVYLNGTGGGLSH"
     gene            complement(5504..5809)
                     /locus_tag="SI621_003159"
     CDS             complement(5504..5809)
                     /locus_tag="SI621_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065838.1"
                     /translation="MLIDKLKSSPMYPTAEMGKQSKKNHWYVREKGSDQPQDQTWRAW
                     WESRSLGKGHINWRSTCVAENVLDPFNPPSRFEVDFKAPDGSIYNLEFALAPHGPNK"
     gene            5948..6244
                     /locus_tag="SI621_003160"
     CDS             5948..6244
                     /locus_tag="SI621_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HEI4065839.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6250..7035
                     /gene="bet"
                     /locus_tag="SI621_003161"
     CDS             6250..7035
                     /gene="bet"
                     /locus_tag="SI621_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEI4065840.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7032..7712
                     /locus_tag="SI621_003162"
     CDS             7032..7712
                     /locus_tag="SI621_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEI4065841.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMANFDEIVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            7709..7891
                     /locus_tag="SI621_003163"
     CDS             7709..7891
                     /locus_tag="SI621_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HEI4065842.1"
                     /translation="MKHPRDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            7864..8055
                     /locus_tag="SI621_003164"
     CDS             7864..8055
                     /locus_tag="SI621_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HEI4065843.1"
                     /translation="MHKASPVKLRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            8132..8347
                     /locus_tag="SI621_003165"
     CDS             8132..8347
                     /locus_tag="SI621_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEI4065844.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGAFKER"
     gene            8446..8667
                     /locus_tag="SI621_003166"
     CDS             8446..8667
                     /locus_tag="SI621_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEI4065845.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHLAVSATHCCECGDPIDE
                     RRRLAVQVCRTCASCQQDLELIRKQRGIK"
     gene            8667..8999
                     /locus_tag="SI621_003167"
     CDS             8667..8999
                     /locus_tag="SI621_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEI4065846.1"
                     /translation="MANLQLPVKSEFFDVMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSDLP"
     gene            8977..9255
                     /locus_tag="SI621_003168"
     CDS             8977..9255
                     /locus_tag="SI621_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HEI4065847.1"
                     /translation="MFRVIYPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9454..10617
                     /locus_tag="SI621_003169"
     CDS             9454..10617
                     /locus_tag="SI621_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEI4065848.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKELPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESTEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(10633..10709)
                     /locus_tag="SI621_003170"
     tRNA            complement(10633..10709)
                     /locus_tag="SI621_003170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10673..10675),aa:Arg,seq:tct)
     gene            10951..11583
                     /gene="fimZ"
                     /locus_tag="SI621_003171"
     CDS             10951..11583
                     /gene="fimZ"
                     /locus_tag="SI621_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HEI4065849.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNNELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(11586..12101)
                     /gene="sfmF"
                     /locus_tag="SI621_003172"
     CDS             complement(11586..12101)
                     /gene="sfmF"
                     /locus_tag="SI621_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HEI4065850.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLSAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASQTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(12112..13119)
                     /gene="fimH"
                     /locus_tag="SI621_003173"
     CDS             complement(12112..13119)
                     /gene="fimH"
                     /locus_tag="SI621_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HEI4065851.1"
                     /translation="MKIICRLLLAMACLCLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13132..15741)
                     /locus_tag="SI621_003174"
     CDS             complement(13132..15741)
                     /locus_tag="SI621_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HEI4065852.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNAYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(15772..>16375)
                     /gene="fimC"
                     /locus_tag="SI621_003175"
     CDS             complement(15772..>16375)
                     /gene="fimC"
                     /locus_tag="SI621_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HEI4065853.1"
                     /translation="ATRIIYPADAKQTAVWIRNSHTNERFLVNSWIENSSGVKEKSFI
                     ITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDKNALNGRNVLQLAI
                     LSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNLQVGSQKLGNA
                     MAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
BASE COUNT         4525 a         3906 c         3776 g         4168 t
ORIGIN      
        1 caataccgac tcctgaccag atagcataag caatccctgt aggaatataa gccagcgtct
       61 gagctaataa ccagaatgat gcacaataac aaataattgt accaacagat ggccataacc
      121 gtgtaaaacc ttctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcac
      181 caccaagata aatataaggg ttcatagcat attctttcct gttcaaactg gagagaattg
      241 tactacagtt tgaaatcaac tcacctgttt catcattgtg tacccattga tgttctttta
      301 tataccctca ataagcgttt catcgcggca ctctggcgac actccttaaa aatcagattc
      361 gtgctcacct ttccttcccg ttcttctctg gtagcgaacc ggtaatacac cgttcgccag
      421 accttaccat caacgaccag gattcctgcc cgcgccattt tagccgcagc ctgatttatg
      481 ctggttacgg ttgcgcctgt taccgcggca acgtcctgtg cacagaagct cttatgcgtc
      541 cccaggtaat gaataattgc ctctttgccc gtcatacact tgctcctttc agtccgaact
      601 tagctttaat ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca
      661 taacaggaag ttgttttact ggttcaggga tcgcctcacc acggttaatt ctcgcagtca
      721 tatggacaag ctcatctgcg gccttacggc gtaattccgc atcagtaagc gcattggccc
      781 gcatgttctg atacaggttg gtaaccagcc agtagtgcgc gtttgatttc cacggataag
      841 actccgcatc cggatacagg cctcgcttcc ggcaatactc gtaaaccata tcaaccagct
      901 cgctgacgtt tggcagtccg gcgataacgg atgcttcttc ccggcaccat gcaacaaact
      961 gcccgggtga tggcagaaat ggtcgattct gccgacgggc tacgcgcatt ccagcgttaa
     1021 cctgttccat tgtggtgatc ccgttttccc ggaaagccag aacccactgg cggcggattt
     1081 cgttcagttc attctggtca cggttagcca ggctcgccgg gaaagttgcc agtaactggc
     1141 tgaacacacc attgatgatc tgcgctacct gctgtacctg aggcttttcg tcgtactgtt
     1201 ccggcatgtt gttggcgatc cgacgcatct gctcacggtc aaagttagtc atctgtgcgg
     1261 cgatgttttt catagatcca ccccgtaaat ccagtctgtg tttgtcaggt cgagttttgg
     1321 tttgctagct gtcacgcctg cctgttgctt gttacggttg atttcgagtt gggtccactt
     1381 gtcgcggagt ttggccggac ttagcacgtt accggaccag aagttgtcct ggcatgccca
     1441 gcggaacagc acgcacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat
     1501 atcgttagcc caccctgcaa aattcggttt tctggctgat ggcgcgatgg tcttcaccat
     1561 gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc ccccacctgc tgccgctctg
     1621 aattgcagca tctggtttct ccacaggaag gtcgttttct ggctggtcag aggattcgcc
     1681 agaattctcg gacgaaaaag gttttatatt gtcttttgtt agtttgtctt ttgtgtttac
     1741 ctgattcggg taaacgtctt tacctgattt gggtaaactt tttttacctg attcaggtaa
     1801 atttacctct ttcaggtaaa ctttattttt cttacctgat tcgggtaatg ttgaccattc
     1861 actgaccaca ttattaatgc cggtattccg cccgctctga ataagaatcc cacgctttac
     1921 cagaacactt tttgcagcag aacatttgtg cggcaatatc ccggtcaact cggaaagttg
     1981 ctcgttgctc acccaatcca gttttttatt aaagccatat gttttgcgca tgacagccag
     2041 gaagaccaga agctggtgct gtgttaatcc ggccagcatt acagcttcca gcaactcatt
     2101 tgcaatgcgc gtataaccat catcgagatc tgccacgcgc ggctcctttt gtgccgcatc
     2161 cggcactgga aaattgaata tctcagcagt gtttgccata attcctcccg caatgagtgt
     2221 gttacgattt gcacctgaaa gtcggttctg ttcccgcaga ccgactttcg ccatttttga
     2281 acctgtcata ttgcccccag catggtggtg accatcgcca tcaatggacc agccagatcc
     2341 gggtccactc gaaacatcga cacaatgcct tcactcatct ccttcagttt ctggtggcgt
     2401 ggtgcgttga gaatgaccgc ctgctttgcc tcactgagtt ccttttccat ttcagccagc
     2461 cgagccatga agctatcctg ctcaaccagg tggccgcgat attccagcgg taataccgcc
     2521 agaattgccg gggtcagttc acgcacgtta tttcggtatt tttcagaatc gaatttgtta
     2581 tcgaggaagc ggaacagctt ctggcgtgca cgactgacat catcaggaaa atcgatggtg
     2641 ccgccgccct gtgctcgata ctcattcaca atgagtgcgg caactacatc ctgattatct
     2701 tcagcagacc aggcgcggac ggcatcacga attttttcgt ggcctggcac ctgttttatt
     2761 tgagaacgat ttatcaccgc agtcgggcta aatccgctag tctgttggta tgtaagtggt
     2821 tgcataatca tcgccttatc agttaacgcc gcagattagg cggcagaatt actcgcgtta
     2881 aacaatggtg cgaggtcggg acgaatatct gctggcttaa tctttccacc agtggctgag
     2941 acaattttca ttacatagcg ggcatcaatt ccgccaccgt gtagccaacg ccaaacagtg
     3001 ggctgggcta caccgcatag atctgccagt cgtttttgac tacctgtaat actgattgcg
     3061 agttgaatgg tttgatttgt cattatcaat tcctattggt attacaatga atgaataata
     3121 gcaatgcgta ttaactatga caatagcaaa acgtgttttg accatcaata cgcaagcgta
     3181 taaattaaaa cttatgaaaa aagaaactct tgctgatcgc ttaaacctag cgatggaaca
     3241 atctgggatg tctcaaggcg ctcttgcaaa ggcgtctggc gtagctcaac caacaatctg
     3301 gagactgaca agcggcaacg cgcgcggctc aacaaaaatt gttgaaatag ctaatgcatt
     3361 gggtgttcga acagagtggc tctcatcagg cataggcccg atgagaaatg acggtcaaca
     3421 atcagggaag cctactgtca accattccaa atacttcaag attgacgttc ttgatataga
     3481 agtgagtgcc gggccgggag tcatcaaccg tgagtttgta gaagtcctac gctcggttga
     3541 gtactcgttt gacgatgctc gtcacatgtt cgatggcagg aaggcggaaa atatccgcat
     3601 cattaacgtg cgtggtgaca gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt
     3661 tgatatcacg gttaaatctt tcgacggtga tggcatctat gcgtttctgt acgacgacac
     3721 cgcccatgta aagcgcctgc aaatgatgaa ggataagctg ctggttatct ctgataacaa
     3781 gagctactca ccgtgggacc cgatcgagaa agacgagatg aaccgggtgt tcatcttcgg
     3841 taaggttatt gggagcatgc cccagacgta caggaaacat ggataatcag tactgtgctg
     3901 atgagtcgtt taggggatag taaatttaat tagaattaga cgagagcgat ttatggattg
     3961 tgacgcttta caggatataa aaatctctct taggtatgac ggaaaagatg ctttaaatca
     4021 tgaaatagat ttgaactgcc taggagaatc cctaaagggt ttttctaaag ttctctcaac
     4081 agcagcttct ttctctgtta cacaaaaata tagtaaatac attaattatc aggaagttaa
     4141 ggtttacgca cgcgaagcaa aagctaactg ctttactctt gaagcagttc ttaacttcgc
     4201 cactcagaac cagttgttct cggggatcgc cgcaactata cttggcgcaa tactacaata
     4261 tatttttgcg agaaattcta acaagaaaga tgaaatgaaa gctttgcagc agtcacttga
     4321 aaaggccata gaggcactag gaaacaagga tgctggaacc attgataagt tgatctcttt
     4381 gattgaccga atggctgtag agcttcgccc ctctgtaagg caggcagtat cacctattgg
     4441 taatacttgc gatcagataa gtgttgcaac aaatgttgac ggctgccttc taaaagttaa
     4501 cgagagagat aaagctgaaa ttgataggct tgatgatgat gaagttctcg gtcttcgtga
     4561 gtatcgtgct tttcttacag aatttgatgc acaaaatatg acagctaaaa taattttaga
     4621 tggtgatgac tcaaaaaaga gaatcactgc tgaaattagc gatccagctg caggaaagaa
     4681 gaataacccg tatattagag ctcttagtgc atatatatca accaaaggtg atccatctgc
     4741 agtattcact ataaccgcaa aggctactgt taaaaaaggc cagataaaca ggttatttat
     4801 tgtagatgcg aaatgatttc cccggccgtc gtgccgggtt ttcttttgcc tcccctcatc
     4861 acacaccgtt caaaaaacca ccacaacccc cgcttcagtt atcgctatgc gatgcaagtc
     4921 acaaaattaa ttccttttgc tatcaaacag ttaatatcaa aatgcatcaa tcaatagcaa
     4981 taagtattga tatcactaat agcaatagct attatcacca tgtcgcaaca acacaacgat
     5041 acggcaacca cctgattcgc cgttgcgatg accgcttaga tccgcagctt gaatttcagc
     5101 aggctccggg gagtgcgagg ggtgaagcgg acgcgtgaac gtcggtgtga ccagctgaaa
     5161 tcaactcaac acttcatacc tcagtcgctt caacgaggcg gcttagttat gacaaccggc
     5221 ggccatccac cgcctgaata cgcgcagaag tctctatatg ttcagcagcc cagcttacgg
     5281 gcaggagttt ttatggttca tcaacattac ggaactcaga ccgttaatcg cggtgccgtc
     5341 cggccaggta tgctggttaa acacaaagat ggtacctgga cggcatcagc taatttgcgc
     5401 ggtcggcttt acttgcatcg cggcatcgaa cgaacgtaca caaaagattt gctcgtggag
     5461 gtttacctga atggtactgg cggtggtctg agccactaac tactcacttg tttgggccgt
     5521 gtggagccaa agcaaattca aggttgtaga tactcccatc aggggctttg aaatcaacct
     5581 caaaccgaga tggcggattg aatggatcga gtacattttc tgctacgcat gtagatctcc
     5641 aattgatatg gcctttgcca agcgagcgag attcccacca agctctccag gtctggtctt
     5701 gcggttgatc actccccttt tcccttacat accaatgatt ctttttcgac tgcttaccca
     5761 tttctgcggt tgggtacatc ggagaagact ttaacttatc aatcagcatt ctagacacct
     5821 tatttatcag attttgagca agacatagtg tgatccaaca ttttgattaa tcaagcgagt
     5881 tcattcaaac aactatgcag ttaacgcact gtctggatcc gcacatcaaa atttcaggag
     5941 tttatccatg aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca
     6001 ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
     6061 cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag
     6121 caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
     6181 acacatccgg tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt
     6241 ataaaacgga tgagtaccgc actcgcaacg ctggctggga agctggctga acgtgtcggc
     6301 atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt
     6361 gatgccagcg atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta cggccttaat
     6421 ccgtggacga aagaaattta cgccttccct gataagcaga acggcatcgt tccggtggtg
     6481 ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt
     6541 gagcaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc
     6601 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga
     6661 gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacggcataa agccatgatt
     6721 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     6781 attgtcgaaa atactgcata cactgcagaa cgtcagccag aacgcgacat cactccggtt
     6841 aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat
     6901 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgcgc atcgtcagaa
     6961 ctgacacagg ccgaagcagt gaaagctctt ggattcctga aacagaaagc cactgagcag
     7021 aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg
     7081 tcgaacaggg ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     7141 ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct
     7201 acttccacac cctgctggct gaggtttgca ccggtgtggc tccggaagtt aatgctaagg
     7261 cgctggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgag ttcacttccg
     7321 gcgtgaatgt tattgaatcc ccgatcatct atcgcgacga aagtatgcgt accgcctgct
     7381 ctccggatgg tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc
     7441 gggatttcat gaaattccgg ctcggtggtt tcgaggccat aaaatcggct tacatggccc
     7501 aggtgcagta cagcatgtgg gtgacgcgaa aagatgcctg gtactttgcc aactatgacc
     7561 cgcgtatgaa gcgtgaaggc ctgcattatg tcgtggttga gcgggatgaa aagtacatgg
     7621 cgaattttga cgagatagtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa
     7681 ttggttttgt atttggggag caatggcgat gaagcatcct cgcgataata tccgggtagg
     7741 cgcaatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gtctttctgt
     7801 tatccgaaat cccctgaaag cacagcggct ggctgaggag ataaataata aacgggaggc
     7861 gatatgcaca aagcatctcc tgttaagtta agaacgagta ttgagatggc acatagcctt
     7921 gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga atttcatacg
     7981 ttagccacat ccctttcaca aaagctggaa atgatggtgg cgaaagcaga agcagatgag
     8041 agagaccagg tatgacaacc actgaatgca tttttctggc agcgggcttc aaattctgtg
     8101 tgcttatgct tgccgacatg ggacttgttc aatgacacct cagcaagaaa acgcccttcg
     8161 cagcattgcc cgtcaggcta attctgaaat caaaaaagcc agacagcagt ttccggataa
     8221 aaacgtcgat gacatttgcc gtagcgtact gaagaagcac cgcgaaacgg taacgctgat
     8281 gggattcaca ccgactcatt taagtctggc aatcggtatg ttaaacggcg cctttaagga
     8341 acggtgaaca tgaaaagcaa aatcctcagg gagctacagg ctcctttttt attgttcgca
     8401 ttcaccctca agcgtattaa ccaacaattc agggattaat ggaagatggc agacatcatt
     8461 gattcagcat cagaaataga agaattacag cgcaacacag caataaaaat gcgccgcctg
     8521 aaccacctgg ctgtatctgc cactcattgt tgtgagtgtg gtgatccgat agatgaacga
     8581 agacgcctgg ccgttcaggt ttgtcgaact tgcgccagtt gccagcaaga tctggagctt
     8641 atccgcaaac aaagggggat taagtgatgg ctaacctgca acttcctgtt aaaagtgaat
     8701 ttttcgatgt catgattcgc ggagagaaaa cggaggagta tcgcctgtgt aatgactatt
     8761 ggaataagcg aattatgttc cgggagtatg accgcctgat tatcacaaag ggatatccaa
     8821 agcgcgacga ttccagtcgc agaattgatg ttccgtatga cggatatgaa gtgaagacaa
     8881 tcacacatcc acactttggc gataaaccgg taaaggtgtt cgctataaag gtgaatatca
     8941 gcactgaata tcaatccgca caacacaagg tcaagaatgt tcagagtgat ttaccctaac
     9001 acctggtacg tcgaccacca cggcactccc tgcaaaatcc tgcgttctac ccacaacaaa
     9061 gttcactaca tccgaaaagg cagaacatgt atcgccagca tgttccgctt taatcatgac
     9121 tttgaacctg tgaataaagc tgatgcagat cggatagcag aagagatcga aacggcagaa
     9181 cacattaaga agttacgtga catgcgttca aaaagcagag gtaaccatgg aatcatacag
     9241 cctcacactc gatgaggcct gtcagtttct taagatatcc agaccaaccg ccaccaactg
     9301 gatacgaaca ggccgcctac aggcaacacg taaagatcca accaagccaa aatctcctta
     9361 cctcacaaca cggcaagcct gcattgcggc gcttcagtct ccgctgcata ctgtccaggt
     9421 gagcgcgggt gatggcataa cagaggaaag aaaatgtcac tcttccgcag aaatgaaata
     9481 tggtatgcct cgtattcgct cccgggcggg aaacgaatta aggaatctct tggcacaaag
     9541 gacaagcggc aagctcagga gttgcacgac aagcgaaaag cagaactctg gcgagtagaa
     9601 aagctagggg atttacctga tgtcactttt gaagaggcct gcctaagatg gcttgaggaa
     9661 aaagctgata aaaaatctct cgattcagat aaaagccgga ttgagttctg gcttgaacat
     9721 tttgagggta taaggcttaa agatatctcg gaggcaaaga tttactctgc tgtaagcaga
     9781 atgcataaca gaaagacgaa agaaatatgg aaacagaaag ttcaggccgc catcaggaaa
     9841 ggtaaagaac tgcctgttta tgaaccaaag ccagtatcaa ctcagacaaa ggcaaagcat
     9901 cttgccatga taaaggccat tctccgtgct gcagaacgcg actggaagtg gctggaaaaa
     9961 gcgcctgtca tcaagatacc agcggtcaga aacaagcgag tcagatggct ggaaaaggag
    10021 gaagcaaaac gccttattga tgagtgcccc gaaccactga aatctgtcgt caagtttgcg
    10081 ctggcaactg gtctgagaaa gtcgaacatc ataaatctgg aatggcaaca aatcgacatg
    10141 cagcgacgag ttgcctgggt gaatccagaa gagagcaaat caaaccgcgc cattggtgtg
    10201 gcgctgaacg ataccgcctg taaagtgttg cgtgatcaaa taggcaagca tcacaaatgg
    10261 gtgtttgtac ataccaaggc ggctaagcga gcagatggaa catcaacgcc tgcggtcagg
    10321 aagatgcgca tcgacagcaa gacatcatgg ctatcagctt gtcgtcgtgc aggaattgaa
    10381 gatttccgtt tccatgacct cagacacacc tgggcaagct ggctgattca gtcaggcgtc
    10441 ccattatcag tgcttcagga aatgggcgga tgggagtcca cagaaatggt tcgtaggtat
    10501 gctcaccttg cgcctaatca tttgacagag catgcgagga aaatagacga catttttggt
    10561 gataatgtcc caaatatgtc ccactctgga attatggagg atataaagaa ggcgtaactg
    10621 attgaattgt aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    10681 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    10741 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    10801 gaccgcgtct gattttctga tttattcgct atagcggcaa acaaacgcac atcgctgcgc
    10861 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttcccg ccctaacgga
    10921 aaattgtccg atccaatgag actggtaact atgaaaccaa cgtcggtgat cattatggat
    10981 actcatccta tcatcagaat gtctattgaa gttctgttgc aaaaaaacaa tgaattgcaa
    11041 attgtcctga aaacggatga ttatcgcata accatcgatt atctccgaac ccgtcctgtt
    11101 gatttaatta ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
    11161 atcaaacaaa tccagagcac agtgaaagta ttatttttat catcgaaatc agaatgcttt
    11221 tatgctggca gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag
    11281 aatgatattt ttcacgccgt tcagatgatc ctctccggat acactttttt tcccagcgaa
    11341 acgctcaact atataaaaag caataaatgc agtacgaata gttcaacggt cacggtgcta
    11401 tctaatcgtg aagtgaccat attacgttat ctggttagcg gattatccaa taaagaaatt
    11461 gccgataagt tattacttag caataaaaca gttagtgcgc ataaatctaa tatttatggc
    11521 aagctaggtt tgcattcaat tgtagagctt atcgactacg ccaaattata tgaattaata
    11581 taatattaat tataattgat cataaatatc gcatccgctt tcgccacacc tggctgaaca
    11641 ccgctggcta acgctcgata attggcaaaa aagtttagtg tgacattgcc atttgcatct
    11701 acttcctcag tctggctcgc ctcccccagt gcgagccggg agcgatcgct attacgtaat
    11761 tcgatggcga cggtttgtgc cattgcggga tcatccagag ccagcaggtt ggcatcagat
    11821 gccggcgttc ccgtaaataa aatcgcaact gaacccggag gacatccctc cagccgcagg
    11881 ctaaaaggga cgagtgccgt ggtatcgcca gcgctcagta attgtgtcgt aggccatctg
    11941 cctaaatcta ccgtcttatc cttatccgct gtgtttacgg tacaggagaa atcaacaacg
    12001 ttaccgtgca aattgatatt aatcgttcct aaagggtcaa ctgcccatcc actggaactc
    12061 cacagtagcc cgcagaaaca gctaaagagt actcttctca ttctccgtac ctcattgata
    12121 atcgacgcgt aaatacccca gcgcgctaaa cggcccttcg gtcggttttt gaccggtaat
    12181 actgataggc caggcgcgaa gtgtgacatt ggctgccgca gctgcatcca gacggaaagg
    12241 aataacgcta ttgagatcgt taggcgtgat cggcgtatcg ttctgatcgg ccacaataaa
    12301 acctaaatcc tgattgtccg acaccatcgc ctgaccagaa acggcactgg cttctagacg
    12361 cattgttaaa taagcctgcg cagcaacatt cgtacatttg atcgcaatgc tcttggtttg
    12421 cggcatgaca ccagctggtc gattccccgg ccctgccgca ctaaataacg atgcgccgat
    12481 atcaccaaaa tcaaattcaa caatttgccc ggcatttaat tcgcagtttt gcggtacttc
    12541 aacccggcca ccaaaactaa tggtataaac aggtgtaacc aacggatcgt taggcgttga
    12601 tgtcacatac acggtaaaca tcacctgtct ggggatctcc accatgttaa taaagggacg
    12661 aataaccttt aaacgaaata tcaaacgaga atccatcacc ccgaacggaa ggttttgcga
    12721 aacgttagga tctgttccca tacgaatata attttgcgga gggaattgaa tatccatcac
    12781 gctatcaacc agactcatcg cacctaatag ataatcattt aattgcatat atttataatt
    12841 atcgatagtt tcctgaacaa taaagttggt gacataacta cggtatgtat aattcaccag
    12901 cgtgccgggt ggacaaattg ctgacacacc tacccagccg gatttttccg gtaaaacaac
    12961 aatctgccct ggctggttat tggtgctatt aaagacattt gagagatcat agtgttcatc
    13021 ttctgctacg ccagtactat ttgcacaaac agtagcccag gatatgtttg tcagacacaa
    13081 acatgccatt gccaataata atctgcagat tatcttcatt gataatttcc tttatgagcc
    13141 aggatgtgtg caaactgccg aaataacagt gacggcttgc tgtaagcttt gcttcggcaa
    13201 tacatagtgc gcaatgcagc gtgagttcgc gccgtctccc cactgaacca gtaattcacc
    13261 agacaatggc gcaccgctta aataaacttg cccgtcatca cccaccatac tggttattcc
    13321 ggtactgttt tcccgtacca gtgatccaaa cggcaccggt tttccgccct gggtaacggt
    13381 aatgagcgcc cgcacgccta tgcgggtatc aaaattggca cgaaccaacg cgccttgcgt
    13441 cggcacaacg ctgctaatat ttttttcaac atcgatggaa ttccccatcg tattggtatc
    13501 aagcgcgata cggttatacc gataaaccgt ggcatacggc atcaccgcat agccgcgcca
    13561 gtcggttaaa atgccagttt gattttcaat gcgtacaccg cctgcgccag gcgctttaat
    13621 caaaacattg gtatccccta aaggctggct cagcgttata ccattttcat gcccgaccac
    13681 accgccagac agctgccagt taacgtcatg ttgatcgcgg tcgtagttat acccgacccc
    13741 cagcgtaccg taagcggcct gccagtttgc cgtggcgctg cccgtatacc cattattcga
    13801 ggtatcgccc tggcttacgt gataactcag gttgtggcct tccagtaagg tcccaccaac
    13861 gcctgccagc cagctatttt gcccgttgct gttacggttg gcgttaaaag aggcataagc
    13921 gcggtcgagc gcattttccc gggtgtagcg acgtttggtc agaacattga aaggcactga
    13981 aacattaagt ccgacaatac gttcgttatc ggggatccct acagattcat tccacgaaaa
    14041 tgagagtgaa taactgatgc caacccagct gctggtatac cccacctgat accaggtatc
    14101 tgaatccgaa gtattccagt atttttgatg ggtaccagaa atataaagcg agccaaagtc
    14161 attaagtgat tgtgaaatat ttaactgcaa acggtcttta cgactaaagc gtaaattgtg
    14221 gtaattcacg attatcggct catcgcgatg ctcaccgtca taatcgtaat catattcgta
    14281 cccctccata cgacgatacg caacatcatc aagggtataa aaaccttgtg tcgaatagcg
    14341 gtaacccatt aactgaaaat tggtgccgaa agtgttcatt gatttcgcat agaggaagcg
    14401 aatggaatcc ccctcatgac gactgtcgtc ggctaactga ctgcgcgcat gcgttacatc
    14461 cagcgacact gcgccccagt tcccgagatt gcgccccagc cctaataaaa aggcggtgta
    14521 atttgcagat aattgcgtcc cgccgtaggc agtaaattcc tgtggtaaac cgccgagtgc
    14581 cgtgccctga aaaaagaaag gcgacgattg ctgactatta ccgctacgaa aatcgcccgc
    14641 cgtcaggtca aatttgaaac gcccttcgcg ttgtaaaatt ggcactgttg aatacggaat
    14701 cgtgtaattc tgctgattac catcgcgctc gtcgatggtg acgtccagat cgccatttga
    14761 agatgtcggg tgcaaatctg taatttcaaa agcgccgggg gaaacatagc tttgatagat
    14821 aataaaacca ttttgccgaa tcgttagctg ggccgccgta cgggcaatcc cacgtaccgt
    14881 tggggcaaac ccttgctggc tatcaggata catattatcg gaagagtaaa gccgtacacc
    14941 acgaaatcca acgccatcga aaatatcact tcctgtattg ccgtctccca ttaccagttc
    15001 acttttcagc ggaataatgg cgcgctgtac ccaggtgcca atattattcc actgttctga
    15061 atgatatgca tttccgcgaa aatagttcca ggaaccattg ttgcgtaatc gccacgggcc
    15121 aatattaatc ccgctgagtt cactaaaaaa atagctatcg ttaccattac ctctgttacc
    15181 ggtgaaatta taattcagga gtaaagcagg aataccttca tcccactctt caggcggaat
    15241 gtaaccgtga gcgctactca acaacgctat ctgaggaata gtgatgttca ggcgcatcgc
    15301 tgcaaaatca aagttaattg tggcatcagg tacagctttc agtaaattaa tgcatttatt
    15361 attttgctgc tgggctaact cggggaatgc actgctatta aggccaattc gttcaaggag
    15421 tgcctgatta aaacaaggca ttaacccacc tgatttatca cctgtattta ctgtcgtcga
    15481 ttcaaatacg atatcctgtg aaccaatgaa ctcatcatta cgccagagat ccacccgata
    15541 cacgcccgca ggttgatgat tacctctctc aaagcgcgat aaatcagcaa cggatgtgcc
    15601 attctctaat aaaaatgccg ggttgaaata actttccgca tagcttaaaa aggagggtaa
    15661 aatggcaaga gatgaataac aaattccggc cagacaccag gtatacctct gcggaatatc
    15721 cgtagtagtg ggtattttca taattagctc tgatattgtc agatgattcg gttagtttaa
    15781 gttcacttct ctgaccgggg ttactgaacc ataatcatta acggtctgaa atttcagctt
    15841 tccctgcact cctgagggta agggaatttg tgaattaact ctgggtgcag ccatagcatt
    15901 ccccagtttt tggctgccca cttgcaagtt aaccagggtg acataaaaag gtgatggatt
    15961 atgaacattg atatagttac cggatcgcga aaacttgagt gtgtccggcg cttctgcggg
    16021 taattcttgt aattgaattg gacggagaaa taatttcatg cgcgataaaa tcgccagttg
    16081 caaaacattc ctgccgttca atgcattttt atctaccgaa gggatcgtct taacattcat
    16141 ccagaacaga gactcacgat ctgccgccag cggtggaccg gtgtaaataa tacgcaaagt
    16201 attttcgctt ttgggttcac taacaaacag tggcggtgta atgatgaatg acttttcttt
    16261 tacaccgctg ctgttttcaa tccacgaatt gaccagaaag cgctcattgg tatggctatt
    16321 tctaatccat accgcagtct gtttagcatc agcgggataa ataatacgcg tggca
//
LOCUS       DARNVM010000109        15934 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.109, whole genome shotgun sequence.
ACCESSION   DARNVM010000109 DARNVM010000000
VERSION     DARNVM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.109"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(304..819)
                     /gene="pgpA"
                     /locus_tag="SI621_003176"
     CDS             complement(304..819)
                     /gene="pgpA"
                     /locus_tag="SI621_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEI4065854.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(797..1774)
                     /gene="thiL"
                     /locus_tag="SI621_003177"
     CDS             complement(797..1774)
                     /gene="thiL"
                     /locus_tag="SI621_003177"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEI4065855.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADVKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGASIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(1852..2271)
                     /gene="nusB"
                     /locus_tag="SI621_003178"
     CDS             complement(1852..2271)
                     /gene="nusB"
                     /locus_tag="SI621_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEI4065856.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2291..2761)
                     /gene="ribE"
                     /locus_tag="SI621_003179"
     CDS             complement(2291..2761)
                     /gene="ribE"
                     /locus_tag="SI621_003179"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEI4065857.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2850..3953)
                     /gene="ribD"
                     /locus_tag="SI621_003180"
     CDS             complement(2850..3953)
                     /gene="ribD"
                     /locus_tag="SI621_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEI4065858.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMESGESQWITSPQARLDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQHNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     ILPGLEKLADALQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3957..4406)
                     /gene="nrdR"
                     /locus_tag="SI621_003181"
     CDS             complement(3957..4406)
                     /gene="nrdR"
                     /locus_tag="SI621_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEI4065859.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4557..4685
                     /locus_tag="SI621_003182"
                     /pseudo
     CDS             4557..4685
                     /locus_tag="SI621_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            complement(4730..5938)
                     /locus_tag="SI621_003183"
     CDS             complement(4730..5938)
                     /locus_tag="SI621_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEI4065860.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFADACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVSPAYTSQRC
                     TCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            5946..6473
                     /gene="tnpA"
                     /locus_tag="SI621_003184"
                     /pseudo
     CDS             5946..6473
                     /gene="tnpA"
                     /locus_tag="SI621_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            6434..6844
                     /locus_tag="SI621_003185"
                     /pseudo
     CDS             6434..6844
                     /locus_tag="SI621_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            7142..8026
                     /gene="tsx"
                     /locus_tag="SI621_003186"
     CDS             7142..8026
                     /gene="tsx"
                     /locus_tag="SI621_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HEI4065861.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(8203..8550)
                     /locus_tag="SI621_003187"
     CDS             complement(8203..8550)
                     /locus_tag="SI621_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HEI4065862.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(8679..9650)
                     /gene="secF"
                     /locus_tag="SI621_003188"
     CDS             complement(8679..9650)
                     /gene="secF"
                     /locus_tag="SI621_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEI4065863.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(9661..11508)
                     /gene="secD"
                     /locus_tag="SI621_003189"
     CDS             complement(9661..11508)
                     /gene="secD"
                     /locus_tag="SI621_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEI4065864.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(11536..11868)
                     /gene="yajC"
                     /locus_tag="SI621_003190"
     CDS             complement(11536..11868)
                     /gene="yajC"
                     /locus_tag="SI621_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEI4065865.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(11891..13018)
                     /gene="tgt"
                     /locus_tag="SI621_003191"
     CDS             complement(11891..13018)
                     /gene="tgt"
                     /locus_tag="SI621_003191"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEI4065866.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(13074..14144)
                     /gene="queA"
                     /locus_tag="SI621_003192"
     CDS             complement(13074..14144)
                     /gene="queA"
                     /locus_tag="SI621_003192"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEI4065867.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRDKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMSAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            14363..14944
                     /gene="acpH"
                     /locus_tag="SI621_003193"
     CDS             14363..14944
                     /gene="acpH"
                     /locus_tag="SI621_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEI4065868.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(14965..>15934)
                     /locus_tag="SI621_003194"
     CDS             complement(14965..>15934)
                     /locus_tag="SI621_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065869.1"
                     /translation="LKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFIAGK
                     VNADNFNVKEFKDDIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTNLEDAQRDLKT
                     TPVDIDGVSLKPKTREKIAEKIAKKTEEENAKKIAKQNAKTIEEKNSEKNKKETNPED
                     NFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLYNVARNGSPRYA
                     GLLRQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHIN
                     DNGELSRTVLMFEGVAESNFKDIANHSSECFSYSCYAAPLDANAKK"
BASE COUNT         3906 a         4235 c         3824 g         3969 t
ORIGIN      
        1 agacaaaata gatgaacttt ctaaaaattt attcaacact ataaaccaaa ccattaagga
       61 taactttgat gatatttttc ggcaacagtc agagaatatg agcaattatt atgagttcgt
      121 cgactgacga tatttgagtg tacgtgaatt atgagtgaca aagatacacc ttaaaatatc
      181 gccgtataat atattcggtg atgtcgggtt ttactttaac agagtgcccc aaaattgggg
      241 cactgttcat ttataaatca aaccgggtgg tctggaatcg cctacacctc ccggtcaatg
      301 gcattaaaga atacccagcg gccagtgatg accgataaag tacaggatgc ctgcggaaat
      361 taccccggcg acaatatcgt cgatcatgat ccccatgccg ccatgtacat tgcggtcaaa
      421 ccagcggatc ggccacggtt tccacatatc caggatacgg aaaatcacaa acccggcggc
      481 gacccactgc cagtcattgg tcggcagcgc catgagcgtg atccacatac caataaattc
      541 gtcccagaca atgctgccgt gatcgtgcac gcccatgtct ttcgccgttt gatgacaaag
      601 atagacgccg atacagatcc ccagcatcac caccagcgaa tagagctgcc agggcaaaaa
      661 ggtcatcaga taccagaacg gaatcgccgc cagcgatccc atcgtgccag gaacgattgg
      721 gcttaatcca cttccgaatc cgacagcaag cagatgccac ggattactca tcttcaggcg
      781 acttttcgcg acatctttat ggcgtggcaa aatggtcata tcctttccag tcgaacgtga
      841 caggttcgcc gtcacgaata aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac
      901 aggtaaacgg tacgcccaga tgtccgagag ccacatccag cgcgccacgg ttcagttccg
      961 gcaccgtgaa acacaactcg taatcttcac cgccagagag cgcccagcgc agcgcctgtt
     1021 ccggttcaac atggcgagaa agcgcatcag aaaacggcag caatgccagg tcaatacttg
     1081 cgccgcagtc gctggctttc acgatatgcc cgagatcgga aatcagaccg tcagagagat
     1141 cgatagctga atttgccaga tcgcgcagtg cctgtccctg taaaatacgc ggagatggac
     1201 ggagatgacg tttgatcaag tagtccgcat ctttaacatc ggcaacctgc aaacggtttt
     1261 gcaaaatcgc cagcccggcg gcgctatcgc ccggtgtacc ggtcacatag atccagtcac
     1321 ccggtttcgc cccagcgcgc gttaaggctc gtcccatcgg aacaaagccg tggataccca
     1381 gcgtcattga taatggccca cgcgtggtat cgccgccaat gagttgcata tcgtaataat
     1441 tgagaagatc aaacaaactg tcgctgaagg actcaagcca cgcttcgtcg acgtccggta
     1501 aggttaatgc cagcgtcagc caggccggat cggcccccat cgcagccaga tcgcttaggt
     1561 tcaccgccag tgctttataa gccagatcag caggatcgat atcagggagg aagtggttgc
     1621 ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt ctcggggata ttgagaagtg
     1681 cgcaatcgtc gccaatgccc agttcgacat caagacgaga acttcttaca cggtcaaaat
     1741 aacgggcaat cagggagaac tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag
     1801 aaaaggccgg cgatcgcgga acggtcttcc gtgaatctac cggcctggat atcacttttt
     1861 gttagggcga atcacaggtg ctgctttatc gagtacgccg ttgacgaact tatggctgtc
     1921 ttctgcgccg aacgatttcg ccagttcgat cgcttcgtta atggccactt tgtatggcac
     1981 atcgctacgt ttagacagct cgtacagcgc aatgcgcagt actgcttttt ctacctggcc
     2041 cagttcttcc agcaggcggg acaggtatgg cttcatcagt ccgtcgaggt atgcggtgtt
     2101 agtcgccacc ccggccagca gctcacggaa gtacaggacg tcaacgtctt ttacatcctg
     2161 ttcagccagg aactggtatt caacatcagc gatgtcgttc tgggacaact gccaggagta
     2221 gagcgcctgg acggcacact cacgagcgcg gcgacgagca gcaggtttca cggatttccc
     2281 cttactaatt tcaggccttg atggctttca atacattaat catttcaagc gcggtcagtg
     2341 cagcttctgc acctttgttg ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc
     2401 tttcagtggt cagaacccca aaagcaaccg gaatttcgct gtcctgggca acatgcgcca
     2461 ggccgttgct tgcaccacca gcgacatatt caaagtgggc agtgccacca cgaataaccg
     2521 tacccagcgc aatcaccgcg tcgtatttac cggttttagc cagtgcaccc gccgccagcg
     2581 gcagctcata ggcaccaggc acccaaacaa cggtaatgtt ttcatctttt acctgaccga
     2641 tacgtttcag tgcatcaatt gcaccttcca gcaggctgtc attgataaag ttgttgaaac
     2701 gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac gttagcttca ataatgttca
     2761 ttttcttcct tcgggttcga gtatggcccc gcaggggggc ggattttagc ataatatttc
     2821 gtgcgctgct tccctttcga gccgggagat catgcaccca ctaaatgcag gcaaacatcc
     2881 gggcctacat gacgtatctc tttgaattta aattggaggg cgtcagctaa tttctcaagc
     2941 cctggtagta tgcataatcc acgggcgtcg ctgcctaata gtttaggtgc gatatagaca
     3001 atcagctcat cgactaaacc cgcctgcagc aatgcgccag cgagcgttgg ccccgcttcc
     3061 acccagatgc tgttaatctg ctgtttaccc agttgcatca ttagtacaac cagatccaga
     3121 tgacctttat gctctggaat cagcaaggta cgcaccgttt ccggccactc acgagaatct
     3181 tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc
     3241 acgcgatttt ggctatcaat cacaatacgt accggctggc ggagattgtg ttgcgggtag
     3301 agcccctgag tttgttcatc cagttcagac caacgcaccg ttaaggccgg atcatccgcc
     3361 agcaccgtgg cactgctggt taaaatggca tgactttgtg cgcgcagtcg ttgtacatcg
     3421 agtcgcgcct gaggcgaagt gatccactgg ctttcgccac tctccatcgc cgtgcgacca
     3481 tcaagcgatg cgccaagttt taactgaata taaggaaagc cggtgcgcat ccgcttgaga
     3541 aagcctttat tcaattgctc ggcttcactc atcatcaggc cgtggctgac gtcaatacca
     3601 gcctgttgca ggcgatatag ccctcgccca gctacctgcg ggttaggatc ttgcatcgag
     3661 gcaaccacgc gcgccacgcc cgcagcaatt aatgcatcac aacacggtgg cgtacgacca
     3721 tgatggctac agggttcgag tgtgacatac gccgttgcgc ctttggcttt ttcgccagcc
     3781 atgcgcaacg cgtgtacctc ggcatgcggt tcacccgctc gttggtggta accttcaccg
     3841 acaatttcgc catctttgac aatgacgcac ccgacattcg ggttgggatg cgtggtaaaa
     3901 cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat actcgtcctg cacggcttag
     3961 tcctccaggc gcgcgatctc ttcgccaaat tctttgatat cttcgaaact gcggtagaca
     4021 gaggcaaaac ggatataggc gactttatcg agctttttca attgctccat caccagattg
     4081 ccaatcatct tgctcggcac ttcgcgctca cccgtggcgc gcagctgcga tttaatatga
     4141 ttgattgcca tttcgacatc atcggaactc accggacgtt tttccagcgc ccgcagcatt
     4201 ccgctacgca atttctcttc attaaacggt tcacgcacgt cgttgctttt tacaacacgc
     4261 ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt cattacacac cagacactgc
     4321 cgacggcggc gtacagatga gccctcgccc acgagacgag agtcaattac cttagtgtcc
     4381 acggcgaaac agaatgggca atgcatacgg cgtcctgacc aggtggttaa cagaaatcta
     4441 ttttaccctg aactgacgat acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc
     4501 tttcactgct tttaccctct accattagac taattgcgac gagaaaggga cgacctatga
     4561 atactaacgt ttttcgactg ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc
     4621 agcaaagcga agtgcgacag atgaaacaca gcgtcagcac gctgaaccag gagatgacgc
     4681 aactcattga catcctccac gccctgaagg acgtggatcc ctgctacgtt caggctgtcg
     4741 cctgaatcat ttcggtgggt tcctgcttca acgggcggcc tgactgcacc atccctccac
     4801 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg
     4861 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt tctttcgctg
     4921 tatgaccaca gcacgtgcaa cgctggcttg tgtacgctgg cgaaacagca agcacctgac
     4981 cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag ccctgatcca
     5041 gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg ctgaccgtac
     5101 ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc attgcgtggt
     5161 ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt gcgatacggg
     5221 aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat ttgaccttgc
     5281 ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa ctgtttacag
     5341 gctcaaagac tgtgccatct gacagcgtgg cgagtttagc cacgccagca tccagcccga
     5401 ccattgatgc tgaagggtga accggagttg atacttcact ttctgtctga atactgatgt
     5461 accacttacc gcaggactgg ctgaccgtga catttttcac aacacccgtg acctgacggc
     5521 tattccggta gcgcatccag cccagtttcg gcagaaaaat acggctgttt tcctgatcga
     5581 gcttaacgcc ctgcgggtag cggaatgcat cattctgtcc ccgctttttg aatcggggaa
     5641 aagcagcccg cttctggaag aagtttttgt aagcccgctc aaggtctttc agtgactgtt
     5701 gcaatggctg tgacggggca tctttaagcc attgcgtttc agtggcattt ttccactcaa
     5761 ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca tgattctcat
     5821 tctgaagcgc cagagcacga ttgaaaacga aacgacatgc gtctgcgaag cgcctcattt
     5881 cacactcctg ttgaccacct ggtcttaact ggaatttaaa tgcctgtaat cgtttcacag
     5941 gtcaaatgat actttggtct atgaaaaaag aaaccgatat ccggcgtgga aggcattgtg
     6001 ttttcctgat gcatgttcac ctggtctttg tcaccagata ccgacgccag atttttgacc
     6061 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
     6121 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac taacctccca
     6181 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
     6241 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
     6301 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
     6361 agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
     6421 ttacggcgca ccggataaag aaacagtcaa aatcactcag caaaacaggc tgaatgcaaa
     6481 atccagcagt ggggtttacc ttctgcctgg agcgaaaaca ccagcaagac tggaaagcca
     6541 gatcggtact ttacgtatgt cgctggtgaa tattacgcct gatacagatg gcaccaccct
     6601 gacgctacgt attcagggcg agtcgaatga tcccttgccc gcattcagcg gaactgttga
     6661 atatgggcaa attcagggaa caatagacaa ctttcaggaa atcaatgtgc agaatcaatt
     6721 gattaacgcc cctgccagcg tcctcgcccc cagcgatgtt gatattccgt tacagttaaa
     6781 gggtatttct gtggatcagt taggctttgt acgtatccac gacattcaac ctgttatgca
     6841 gtaaacgttt cgcggggcgt tttgtgcgtc ccgcaacatc tttccccgtc attttgttac
     6901 tctgcttaca tcacccagat tgatagtaaa agtttgcaac aagggtgaaa gtcagtacaa
     6961 tccccgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat agaactgtga
     7021 aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta tacgaaatat
     7081 ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca gtggcataca
     7141 tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt cttttactgt
     7201 caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc agagcgttaa
     7261 cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata cctaccttga
     7321 gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg atgcgccggt
     7381 attcttcggc ggtaactccg atgcaaaagg tatctggaac cacggttctc cgctgtttat
     7441 ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta gctttggtcc
     7501 gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta ataaagatgg
     7561 tcgccagagc acctggtaca tgggtctggg taccgatatc gatactggcc tgccgatgag
     7621 cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag cgaatgaaaa
     7681 cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg atctgtgggg
     7741 cggtcagctg agctacatcg gctttaccaa cttcgactgg ggttccgatt taggggatga
     7801 cagcggtaat gcaatcaacg gtattaagac ccgtaccaat aactccatcg cttccagcca
     7861 tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt actggcacga
     7921 cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact tcaacgttcg
     7981 ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgatgaa tgaaatgccg
     8041 gatcttgtat ccggcatttc agtttgttaa caacgtgcgc tttgttcatg ccggatgcgg
     8101 cgtaaacgcc ttatccggcc tacaaaagca cgtaaattca atacattgct gagattacgt
     8161 tggcctgata agcgtagcgc atcaggcaat tttgcgtttt tatcacttct tcttgttcat
     8221 catcgctttc aggtcagcga atgggttgta cttcgcttca ccgacatctt tctgcgcatc
     8281 ttcccctgcg ataacggtcg taccatactg atctgcttcg gtatattttg aatgctcgtg
     8341 atcgtggcaa taaagacaca ataactccca gttactgccg tcttccgggt tattggtatg
     8401 gtcgtgatca atgtggtgaa cggtaagttc acgcaggttg gaataaacaa actcgcgaga
     8461 acagcgaccg cagacccacg gatagatttt taatgctttt tcgcgatagc cgctttctaa
     8521 ccgcgcatag ttttttggga ttatagccat gaaaagttac ctggatgaaa tgtagggtta
     8581 cgcgcagttt cccaagatgc ggggattttc gcaatctcca tacatcaaaa aaaatcccga
     8641 tcttctgacc gggattttca acatcaacgg gaacttgatt acggcagaat tgacggctga
     8701 tccgcccctt ctttttccac tttctgctgc agcatgtgtt cgcgcttcat acccagtttc
     8761 agagccagcg ccgatgctac gtagatggaa gatgcagtac cgatggaaac accaatcagc
     8821 atggtcagcg agaagccttc cagtaccgga ccaccgaaga ggtacagcat caggataacc
     8881 atcaaggtag taccggatgt gatcaaggta cggtgcagcg tctgggtcaa ggacacgtta
     8941 aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt tttcacgaat acggtccgat
     9001 accacgatac tgtcgttaag cgagtaaccg ataactgaca tcaacgatgc cacaatggtc
     9061 aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac gtcgtgcgcc
     9121 agcgcaataa caacccctgc cgccagtcgc cactcaaagc ggaaacctac gtacacgagg
     9181 atagacagca gcgccgccat caacgccatc gcaccggttt gcgcaaggtc tgcccccacg
     9241 ctcggaccga cgaactcaat acgcttcact gccgcattct gattggtgga ttcgttaatc
     9301 accttcagga cctggctgcc cagcacctga ccgccggttt cgccttcagc aggcggcata
     9361 cggaccatga tgtcgtggct gctaccaaag ttttgcagca tcggctcttc aaaaccggct
     9421 ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt aatttcgata
     9481 accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat aataacgata
     9541 gcggcgatta acagcagacc agagatgcca aaagcccagt agtcccagcg cataaagtca
     9601 tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac atcgcactcc
     9661 tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga tggcacgcgt
     9721 accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa tcgcgaaccc
     9781 tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg tggtgatgtt
     9841 cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct gttgaacagt
     9901 acgtccgttg ctcaactctt ctttaatacg ttcgttgata agtacgttcg catcgaccgc
     9961 taccgcaaga gttaagacga tacccgcgat acctggcata ctcagcgttg cgcctggcaa
    10021 cagcgacata atgccgacaa tcaagatcaa gttggcaatc agagcactgg tcgcaatcag
    10081 accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac cggcaaggca
    10141 agcctccagc ccctgctcaa tgttctgcat acccagggtc gggccaattg tacgttcttc
    10201 aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca gctgacgggc
    10261 ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta cccagacgag actggatgtt
    10321 ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccgtttg catctttctt
    10381 accgctgtct ttgtactcca caaacagggt cgccatcggt ttgccgatgt tgtccttggt
    10441 gaagttagac ataatgttgc cacctgcgct gtcgagcgag atgttaacct gcggctggtt
    10501 gtattcgtcc tggctggaag tggagtcggt gatatggtca ccggtcagaa ttacgcgttt
    10561 gtacagcaca actggctgac cttcgcgggt ctgtttcact tcagagtcgc caggtacgcg
    10621 accggatgcc gctgcggcct gatcaacgtt agtgtttacc agacggaatt ccagcgttgc
    10681 cgtcgcaccc agaatctctt tcgcacgcgc ggtgtcctga atacctggca gttcaacaac
    10741 gatacggtca gcaccctgac gctgaaccac cggctccgcc acaccaagtt ggtttacacg
    10801 gttacgcagg atattaatgt tctgctgcac cgcatattca cgcgcttcac taaggcgagc
    10861 atcgctcatt accgcacgca gctggttgct gccctggcta ctaatcacca ggtccggatg
    10921 acgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga aagtgatgct
    10981 caggccgtag ttgttttctt tacgaacggt ggtatacggg atgccctttt cgcgcaggtc
    11041 gctgcgcagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt ccatatccac
    11101 ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg gctcagcgtg
    11161 aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga cgtatttgtc
    11221 acccataacg cccattaatg cttcacgagc gcgcagctgg gtgtcagtgg agtcgaagcg
    11281 cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt cttcttgtaa
    11341 cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg caccagtgat
    11401 ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac cgatgacaat
    11461 caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg caattccctt
    11521 agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg cagctacgaa
    11581 gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt agccgttttc
    11641 cgctactttg gttacgcgac caaccaggcc accgttcgtc agaacttcat cacctttggc
    11701 aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac gcaggatcat
    11761 gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg ggctaccttg
    11821 cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta aatttccctc
    11881 attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc tggtaaaaat
    11941 cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc gccatcaaac
    12001 gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc gcctaatatt tcgttgcaac
    12061 ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa tcacactcag
    12121 gatcgagtgg gccagtatcg ctcttatact tcgcattgcg gattttcacc acgccatcgg
    12181 tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca aacatatcga
    12241 taccgcgacg cacaccttca accaggtctt ctggtttacc aacgcccatc aggtaacgcg
    12301 gtttgtctgc cggaatttgc gggcatacat gctccagaat gcggtgcata tctgctttcg
    12361 gctcacccac agccagaccg ccgacagcgt agccatcaaa accgatatct accagacctt
    12421 taacagaaat atcacgtaaa tcttcgtaaa cgctgccctg aatgatacca aacagcgcat
    12481 ttttgtttcc gagactgtca aaacgctcac ggctacgctt cgcccaacgc agagacatct
    12541 ccatggagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca tcaaagatca
    12601 tgacgatatc cgaaccaaga tcgtactgaa tctccatcga cttttccggg tcgaggaaaa
    12661 tcgggtcgcc gttgatcggg ttacggaagt gcacgccctg ttcggtgatt ttacgaatat
    12721 caccaaggct gaagacctgg aagccgccgg agtcggtaag gattggtcct ttccactgca
    12781 taaaatcgtg cagatcgccg tgcagtttca tgatttcctg gcccgggcgc agccacaggt
    12841 ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttcc ggcgtcatcc
    12901 cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact acgccacgat
    12961 caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagctca aatttcattt
    13021 tttctccact acgtcagaaa aacagtccaa cgttttaaac cagcgccgcg gaattactcc
    13081 ccgacgcgct cattaattgc ctgcggattg tacgtgataa acatcgcatc accgtaacta
    13141 aaaaagcgat atttctcttc taccgctgct ttataggcgc tcatggtgtg ttgataaccg
    13201 gcaaaggccg aaaccaacat aatcagcgtc gactctggca agtggaagtt cgtcaccagc
    13261 gcatcgacca ctttgtactg aaagcccgga tagataaaga tttgggtgtc gtcaaagaac
    13321 ggttcgatga gatcgttttt cgccgcctga gccgcgcttt ccagtgaacg taccgaggtg
    13381 gtgccaaccg caatcacccg gttaccgcgc gctttcgccg ccagtaccgc gtctaccaca
    13441 tcctgcggta cttcagcgta ttccgagtgc atgatgtgat cttcaatggt gtcgacgcgc
    13501 accggctgga aggtgcccgc accaacgtgc agggtgacga acgccatctc cacgcctttg
    13561 tcgcgtaatt tttccagcaa aggttcgtca aaatgcagac cagcggtcgg ggctgcaacc
    13621 gcgcccggtt tttcgctata aacggtttga taaagttcgc ggtcagcgtc ttcgtccgga
    13681 cggtcgatat acggcggcag cggcatatgg ccgatgctgt tgaggatatc cagtaccgaa
    13741 cgatcatcat taaattcaac ttcaaacagt gcgccgtggc gcgcggtcat tgttgcgttg
    13801 atactttcgt catcgcccag taacagttct gcgccaggtt ttggcgcttt cgaggcgcga
    13861 atatgcgcaa gaatgcgttt gtcgtcgagc atccgctcaa ccagcacttc aatcttgccg
    13921 ccgctggctt tacgcccaaa caggcgcgcc gggatcacgc gggtattatt aaaaaccaga
    13981 agatcgccgg ggttgagctt atcgagtaaa tcggtgaaag taccgtgcgt cagcgcgccc
    14041 gtcggcccgt ccagcgacag taaacgacag ctactgcgtt caggcatggg atagtgggca
    14101 atcagggatt cgggcaattc aaaggagaaa tcggtaacgc gcatgacact gactcagact
    14161 aaaataagag gcgggtagtc tagtgccggg gcgctctccc tgcaaccttt accctccttg
    14221 atggatgaca taatctatac acaaaatcat tcaagatgca tcgagatggc aactgaatga
    14281 gtccccagga gcttacatta agtaagtgac tggggtgagt gaaggcagcc aacgaagagg
    14341 cagtttgaag gatgaagtgt atatgaattt tttagctcac ctgcatctag ctcacctggc
    14401 ggaaagctcg ctttctggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag
    14461 ttttccgccc gacgtcgtgg ctggcattca tatgcatcgg cgcattgatg tactaaccga
    14521 caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaaccc gccgcgttgc
    14581 gcctattacc ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc
    14641 gccggacttt ccgctacagg aatttgtctg ttatgcccgc gaacaagtga tgacgatttt
    14701 gccggactca ccgccgcgct ttatcaatct gaacaactac ttatggtcag agcagtggct
    14761 ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgtcg
    14821 cccacgtctg gatgccctgc gtgactcctg gtacgattta aacgcccatt atgccgccct
    14881 cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac gcaaggcgct
    14941 ctgaaacttc aagctccggc gtcactattt tttagcattc gcatccagcg gggcggcata
    15001 acaactgtaa gaaaaacatt cgctggaatg attggcgata tctttaaagt tgctttcagc
    15061 aaccccctcg aacatgagta ccgttctgga caactctccg ttgtcattta tatgtggaaa
    15121 atcaaattgt gtactgaatc gccatacatc tgtcttttct ggctcgctgt tcacagagat
    15181 acgtcgatgg aagactcctc ctgaaaacgt gcgccagaac aattgttgat caaaatcttg
    15241 taatatcgca cacttggatg ctatttgacg tagtaaacct gcataccgtg gactgccatt
    15301 acgagcaaca ttatacaaac cctgactaaa agcatcaaac aaacaataga tatgatcact
    15361 agtgaaaaaa ggtcttaatt ttttttgttc ttcatcgctg taatccagga gcatctcatt
    15421 gatttcttcc ggtgagaatt ttaagacaaa attatcctca ggatttgttt ctttcttgtt
    15481 tttttcacta ttcttctcct caatggtctt cgcattttgt ttcgcaattt ttttcgcatt
    15541 ttcttcctca gtttttttcg caattttttc cgcaattttt tcccgagttt ttggttttag
    15601 actaacacca tcgatgtcaa ccggtgttgt tttcagatct ctctgcgcgt cttccaaatt
    15661 tgtcttatag agatcgggta ctttatcggc agataagtat ccggatgaag atatctggtg
    15721 tgttcgcatg gggggctgtt cacaaagcat cgaggctata tcgtctttaa actcttttac
    15781 attaaagtta tcggcattaa ccttccctgc tataaacccc gcatcggcaa ttttcttctc
    15841 taattgtttt gtacgtttag aataataagc gttgttatca taaaactttt caagaagggc
    15901 ccactcctca agttgcctta tgtgattttt caac
//
LOCUS       DARNVM010000110        15921 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.110, whole genome shotgun sequence.
ACCESSION   DARNVM010000110 DARNVM010000000
VERSION     DARNVM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.110"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(37..1491)
                     /gene="xylB"
                     /locus_tag="SI621_003195"
     CDS             complement(37..1491)
                     /gene="xylB"
                     /locus_tag="SI621_003195"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEI4065870.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRLLRPAILWNDGRCA
                     QECALLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLNNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLTDISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1563..2885)
                     /gene="xylA"
                     /locus_tag="SI621_003196"
     CDS             complement(1563..2885)
                     /gene="xylA"
                     /locus_tag="SI621_003196"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEI4065871.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3251..4243
                     /gene="xylF"
                     /locus_tag="SI621_003197"
     CDS             3251..4243
                     /gene="xylF"
                     /locus_tag="SI621_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4065872.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKETVVKDGFHKESEL"
     gene            4321..5862
                     /gene="xylG"
                     /locus_tag="SI621_003198"
     CDS             4321..5862
                     /gene="xylG"
                     /locus_tag="SI621_003198"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HEI4065873.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5840..7021
                     /gene="xylH"
                     /locus_tag="SI621_003199"
     CDS             5840..7021
                     /gene="xylH"
                     /locus_tag="SI621_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HEI4065874.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7099..8277
                     /gene="xylR"
                     /locus_tag="SI621_003200"
     CDS             7099..8277
                     /gene="xylR"
                     /locus_tag="SI621_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HEI4065875.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8385..9209)
                     /locus_tag="SI621_003201"
     CDS             complement(8385..9209)
                     /locus_tag="SI621_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HEI4065876.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9529..11559
                     /gene="malS"
                     /locus_tag="SI621_003202"
     CDS             9529..11559
                     /gene="malS"
                     /locus_tag="SI621_003202"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEI4065877.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTINFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11737..12990
                     /gene="avtA"
                     /locus_tag="SI621_003203"
     CDS             11737..12990
                     /gene="avtA"
                     /locus_tag="SI621_003203"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEI4065878.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLRDKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPDKIEAGVKILAEEIERAWAESH"
     gene            complement(13141..13614)
                     /locus_tag="SI621_003204"
     CDS             complement(13141..13614)
                     /locus_tag="SI621_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEI4065879.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13716..14564)
                     /gene="yiaJ"
                     /locus_tag="SI621_003205"
     CDS             complement(13716..14564)
                     /gene="yiaJ"
                     /locus_tag="SI621_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HEI4065880.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWKSHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14765..15763
                     /gene="yiaK"
                     /locus_tag="SI621_003206"
     CDS             14765..15763
                     /gene="yiaK"
                     /locus_tag="SI621_003206"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HEI4065881.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15775..>15921
                     /locus_tag="SI621_003207"
     CDS             15775..>15921
                     /locus_tag="SI621_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEI4065882.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQI"
BASE COUNT         4008 a         4074 c         3940 g         3899 t
ORIGIN      
        1 gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg gcagaagttg
       61 ctgatagagg cgacggaacg tttctcgtcg tggctgataa gcggcataac gctgcgcatc
      121 tggtagatgc gactgttcta acggcagttg tggcaacaac tcaatgagcg atttctctgg
      181 attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat ccccccccgt
      241 gcggtaatcg agttgctgac cgctgatatc cgtcagcatc tgacgccagt actcactacg
      301 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
      361 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
      421 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
      481 acgctcgccg gaaagataag gcagaaacca aactggctcg gcactttcat cagcctgttg
      541 agcagccgcg attaaagctg ggacattgtt caggcccgtt aatttcgcag cccaatccag
      601 acacgacgct gcactcagca tcacagacat taaatgccaa cgctgcggta gcgcatggca
      661 aaagctgtgt acggcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
      721 tcccgacgtc cccagcgata acattgcctg attagcatca accattccta caccaaccgc
      781 accagctgca ttatcaccac cgcctgcgac aactggcacc gccgccatac cccacgcttt
      841 cgcaacttca ggtagcaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
      901 gtcacgagat aagtggcaag cctgcagcat gtcgtcactc cagtcacgct ttgcgacatc
      961 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1021 acgcaagtaa tctttcggta acaatacttt gtcgatttga cggaatatct ccggctcatg
     1081 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt taccggtaat
     1141 cacccgcgat tgcggaactc gcgcttccag caaagcgcac tcttgcgcac agcgcccgtc
     1201 gttccacaaa atggcagggc gtaataaccg ttgctgggca tccagtaagg ttgcaccgtg
     1261 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagggc
     1321 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     1381 atgcgggcgc gaaacggtca gcttttccgt ttgcgcagca accacctcgc cctgctcgtt
     1441 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     1501 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     1561 cgttatttgt cgaacagata atggtttacc agattttcca gttgctcctg gcgaccactc
     1621 tgatgcaccg gagacaaatt gtgttcctga gcatatttgg ctaaatctgc cagtgacatt
     1681 tggcctttca ggatttgctg gcctaattcg ctattccagc cggaataacg ctgcgcgatg
     1741 cgtttatcca gctcgccatc ttcaatcatg cgtgctgcaa ttttcagcgc caacgccatc
     1801 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     1861 actttggcat cgaagttcag accgccggtg gtgaaaccgc ctgctttgag aatttcatac
     1921 atcaccaacg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgt
     1981 gcatcgccac ggttagcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2041 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2101 tctttttcca gaccaaactg tttcaggaag ccatagactg tcgcagcatc gtaatcatat
     2161 tgatgtttgg tcggttcttg cggtttcggt tcgataagca acgtaccctg aaagccgatt
     2221 ttatgtttat gctcaaccac catttgcata aagcggccca gctgttcacg ctcctgacgc
     2281 aagtcggtat ttaacaacgt ttcgtaacct tcacgaccgc cccacaggac atagttttca
     2341 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     2401 acttctggat ctgggttcgt cgccgcaccc gcgccgtagc gagggtttgt aaagcagtta
     2461 gcggttcccc acagcagctt cacgccgctc tcttcttgct tgcctgccag gacatcaacc
     2521 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccacg
     2581 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     2641 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     2701 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     2761 aagtgctctt ccatacgctt gcccaacacc agttcgtcgg gattatagtg atggaatgct
     2821 aacgggttag aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     2881 tgcatattga actccataat caggtaatgc cgcgggtgat ggatgatgtc gtaatattgg
     2941 gcactccctt tcagttgctc aattatgtta tttcacactg ctattgagat aattcacaag
     3001 tgtgcgctcg ctcgcaaaat aaaatggaat gaggaaactg ggtaattcct cgaagagaaa
     3061 aatgcaataa gtacaattgc gcaacaaaag taagatctcg gtcataaatc aagaaataaa
     3121 ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt tcccctgttt agttgctaaa
     3181 aattggttac gtttatcgcg gtgattgtta cttattaaag ctgtcctcta cctacagaag
     3241 gccctacacc atgaaaataa agaacattct actcaccctt tgcacctcac ttctgcttac
     3301 caacgttgcg gcacacgcca aagaagtcaa aataggtatg gcgattgatg atctccgtct
     3361 tgaacgctgg caaaaagatc gagatatttt tgtgaaaaag gcagaatctc tcggcgcgaa
     3421 agtatttgta cagtctgcaa atggcaatga agaaacacaa atgtcgcaga ttgaaaacat
     3481 gattaaccgg ggcgtcgatg ttcttgtcat tattccgtat aacggtcagg tattaagtaa
     3541 cgttgtaaaa gaagccaaac aagaaggtat taaagtatta gcttacgacc gtatgattaa
     3601 cgatgcggat atcgattttt atatttcttt cgataacgaa aaagtcggtg aactgcaggc
     3661 aaaagccctg gtcgatattg ttccgcaagg taattacttc ctgatgggcg gctcgccggt
     3721 agataacaac gccaagctgt tccgcgccgg gcaaatgaaa gtgttaaaac cttacgttga
     3781 ttccggaaaa attaaagtcg ttggtgacca atgggttgat ggctggttac cggaaaacgc
     3841 attgaaaatt atggaaaacg cgctaaccgc caataataac aaaattgatg ctgtagttgc
     3901 ctcaaacgat gccaccgcag gtggggcaat tcaggcatta agtgcgcaag gtttatcagg
     3961 gaaagtagca atttccggcc aggatgcgga tctcgcaggt attaaacgta ttgctgccgg
     4021 tacgcaaact atgacggtgt ataaacctat tacattgttg gcaaatactg ccgcagaaat
     4081 tgccgttgag ttgggcaatg gtcaggaacc aaaagcagat accactctga ataatggtct
     4141 gaaagatgtt ccctcccgcc tgctgacacc gatcgatgtg aataaaaaca acatcaaaga
     4201 aacggtagtt aaagacggat tccacaaaga gagcgagctg taagcgttac gccccggcgg
     4261 ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg tggagtcgtt
     4321 atgccttatc tacttgaaat gaagaacatt accaaaacct tcggcagtgt gaaggcgatt
     4381 gataacgtca gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg ggaaaatggg
     4441 tctggtaaat caacgctgat gaaagtgctg tgtggtattt atccccatgg ctcctacgaa
     4501 ggcgaaatta tttttgcggg agaagagatt caggcgagtc acatccgcga taccgaacgc
     4561 aaaggtatcg ccatcattca ccaggaattg gccctggtga aagaattgac tgtgctggaa
     4621 aatatcttcc tgggtaacga aataacccac aatggcatta tggattatga cctgatgaca
     4681 ctacgctgtc agaagctgct cgcacaggtc agtttatcca tttcacccga tacccgcgtt
     4741 ggcgatttag ggcttgggca acaacaactg gttgaaattg ccaaggcact taataaacag
     4801 gtgcgtttgt taattctcga tgaaccgaca gcctcattaa ctgagcagga aacgtcggtt
     4861 ttactggata ttattcgcga tctgcaacag cacggtatcg cctgtattta tatttcgcac
     4921 aaactcaacg aagtcaaagc gatttccgat acgatttgcg ttattcgcga cggtcagcac
     4981 attggtacgc gtgatgctgc cggaatgagt gaagacgata ttatcaccat gatggtcggg
     5041 cgagagttaa ccgcgcttta ccctaatgaa ccacatacca ccggagatga aatattacgt
     5101 attgaacatc tgacggcatg gcatccggtt aatcgtcata ttaaacgagt taatgatgtc
     5161 tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg gactcgttgg tgccggacgt
     5221 accgagacca ttcagtgcct gtttggcgtg tggcccggac aatgggaagg aaaaatttat
     5281 attgatggca aacaggtaga tattcgtaac tgtcagcaag ccatcgccca ggggattgcg
     5341 atggtccccg aagacagaaa gcgcgacggc atcgttccgg taatggcggt tggtaaaaat
     5401 attaccctcg ccgcactcaa taaatttacc ggcggtatta gccagcttga tgacgcggca
     5461 gagcaaaaat gtattctgga atcaatccag caactcaaag ttaaaacgtc gtcccccgac
     5521 cttgctattg gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc tcgctgtctg
     5581 ttactcaacc cgcgcattct cattcttgat gaacctacca ggggtatcga tattggcgcg
     5641 aaatacgaga tctacaaatt aattaaccaa ctcgtccagc agggtattgc cgttattgtc
     5701 atctcttccg aattacctga agtgctcggc cttagcgatc gtgtactggt gatgcatgaa
     5761 gggaaactaa aagccaacct gataaatcat aacctgactc aggagcaggt gatggaagcc
     5821 gcattgagga gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat tggccgtacc
     5881 gacatccggt ggcttttccg ggttgaagtc actgaatttg caggtcttcg tgatgattgc
     5941 agctatcatc gcaatcatgc tgttctttac ctggaccacc gatggtgcct acttaagcgc
     6001 tcgtaacgtc tccaacctgt tacgccagac cgcgattacc ggcatcctcg cggtaggaat
     6061 ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc ggctcaatga tggggctatt
     6121 aggtggcgtc gcggcgattt gtgacgtctg gctaggctgg cctttgccac ttaccatcat
     6181 tgtgacgttg gttctgggac tgcttctcgg tgcctggaac ggatggtggg tcgcgtaccg
     6241 caaagtccct tcatttattg tcaccctcgc gggcatgttg gcatttcgcg gcatactcat
     6301 tggcatcacc aacggcacga ctgtttcccc caccagcgcc gcgatgtcac aaatcgggca
     6361 aagctatcta cccgccagca ccggcttcat cattggcgcg cttggcttaa tggcttttgt
     6421 tggctggcaa tggcgcggaa gaatgcgccg tcaggctttg ggtttgcagt ctccggcctc
     6481 taccgcagtt gtcggtcgcc aggcattaac ggcgattatc gtcttaggcg caatctggct
     6541 gttgaacgat taccgtggcg ttcccactcc tgttctgctg ctgacgttgc tgttactcgg
     6601 cggaatgttt atggcaacgc ggacggcatt tggacgacgc atttatgcca tcggcggcaa
     6661 tctggaagca gcacgtctct ccgggatcaa cgttgaacgc accaaacttg ccgtattcgc
     6721 tattaacgga ttaatggtcg ctatcgccgg attaatcctc agttctcgac ttggcgctgg
     6781 ttcaccttct gcgggaaata tcgccgaact ggacgcaatt gccgcatgcg tgattggcgg
     6841 caccagtctg gctggcggtg tgggaagcgt tgccggagcg gtaatggggg cattcatcat
     6901 ggcttcactg gataacggta tgagtatgat ggatgtaccg accttctggc agtatatcgt
     6961 taaaggtgcg attctgttgc tggcagtatg gatggactcc gcaaccaaac gccgttcttg
     7021 attttgataa aaattttctc aaagccggta acgtattacc ggttttgagt ttttgcatga
     7081 ttcagcagga aaagaaccat gtttactaaa cgtcaccgca tcacattact gttcaatgcc
     7141 aataaagcct atgaccggca ggtagtagaa ggcgtagggg aatatttaca ggcgtcacaa
     7201 tcggaatggg atattttcat tgaagaagat ttccgcgccc gcattgataa aatcaaggac
     7261 tggttaggag atggcgtcat tgccgacttc gacgacaaac aaatcgagca agcgctggct
     7321 gatgtcgacg tccccattgt tggggttggt ggctcgtatc accttgccga aagttaccca
     7381 cccgttcatt acattgccac cgataactat gcgctggttg aaagcgcatt tttgcattta
     7441 aaagagaaag gcgttaaccg ctttgctttt tatggtcttc cggaatcaag cggtaaacgt
     7501 tgggccactg aacgcgaata tgcatttcgt cagcttgtcg ccgaagaaaa gtatcgcgga
     7561 gtggtttatc aggggttaga aaccgcgcca gagaactggc aacacgcgca aaatcggctg
     7621 gcagactggc tacaaacgct gccaccgcaa accgggatta ttgccgttac tgacgcccgg
     7681 gcgcggcata ttctgcaagt atgtgaacat ctacatattc ccgtaccgga aaaattatgc
     7741 gtgattggca tcgataacga agaactgacc cgctatctgt cgcgtgtcgc cctttcttcg
     7801 gtcgctcagg gcgcgcggca aatgggctat caggcggcaa aactgttgca tcgattatta
     7861 gataaagaag aaatgccgct acagcggatt ttggtcccac cagttcgcgt cattgaacgg
     7921 cgctcaacag attaccgctc gctgaccgat cccgccgtta ttcaggccat gcattacatt
     7981 cgtaatcacg cctgtaaagg gattaaagtg gatcaggtac tcgatgcggt cgggatctcg
     8041 cgctccaatc ttgagaagcg ttttaaagaa gaggtgggcg aaaccatcca tgccatgatt
     8101 catgccgaga agttggagaa agcgcgcagt ctgctgattt caaccacctt gtcaatcaat
     8161 gagatatcgc aaatgtgcgg ttatccatcg ctgcaatatt tctactctgt ttttaaaaaa
     8221 gcatatgaca cgacgccaaa agagtatcgc gatgtaaata gcgaggtcat gttgtagcga
     8281 tgatgagaat tgtcggcgtc gcatcaggta actcagcaca accacctgat gcgagcaaaa
     8341 gccttatccg actgacaagt cggataaggc tcgaggaaat gcaattacat atgcgccgcg
     8401 attaaccgtt ggttatcctg gtacattgcg aacaggtagt tgttataact cttccctttg
     8461 gtcgagtagc ccttcagttt gtgaatcatc gctgtggcag tcacttcctg atccgcttta
     8521 cgcagctgtg cacgcgattt acggaacgaa gagtaagccg ggtgagtatt caggttagtg
     8581 acataggcgc tcaccgattc tttgacagaa ctaaactgtg agtacccctt cactttaccc
     8641 ggcgcattag tacaacgtcc tttcatgcat ttcatgccga acaggttgtt gttgttgcgc
     8701 gccagcttcg acgttcccca accgctttct gctgcagcca tcgtcgccac catactagtg
     8761 gggataatgt ctacgcgttc aagcaaggta ttccacggga ttttgcgcgt attaccagac
     8821 cacttcacct tgtagcgttt ggcgatgtct ttcagacgcg cacgctcagc aggtgaccat
     8881 tggccctgat actgttttga aatgagccag ttacgttccg cagtaatggc cgcattttgg
     8941 ctggtaatgt aaggcattac agtccggaga aacgcctttt tccttggtgt tccggaaggg
     9001 tattttcgca aatcaggaag tgaactactc tttgcactat tgcgagaata ctcttgttta
     9061 ctgcttacct gtttattact agctttagtt aagtgggact tttgactcgc tgttgttgtg
     9121 tgcgtcttcg ctaacacctc actcgaaaac accagagtga gtaacataag aatcatcgcc
     9181 ccatatcgtc gtatgggagt caaaatcatc aggtctcctg gtcggattta atcattccaa
     9241 caccttatat ttttcacaaa tttgagagtt gaatctcaaa tcatatcaaa aatagctgtc
     9301 aagagcaccc caaggaatag tccaaatctg aaactatgtc acgagttaac gattcagatt
     9361 ggcgctaaat cgcagaaaat gtggggttta tcgcaaaatt cagccgtttt ttgcgcgcga
     9421 tcgctcaccc ttgcttctca tcctgtggac ttaccgctca gggatgagtt ttgtctggct
     9481 tatcgctggc aaactgtctg aaatcgcagc aataaggact catccgccat gaaactcgcc
     9541 gcctgttttc tgacgctcct tcctggcttc gccgttgccg ccagctggac ttctccgggg
     9601 tttcccgcct ttagcgaaca ggggacggga acatttgtca gccacgcgca gttgcccaaa
     9661 ggtacgcgtc cactaacgat aaattttgac caacagtgct ggcagcctgc ggatgcgata
     9721 aaactcaatc agatgctttc cctgcaacct tgtagcaaca cgccacctca atggcgattg
     9781 ttcagggacg gcgaatatac gctgcaacta gacacccgct ccggtacacc aacattgatg
     9841 atttccatcc agaacgccgt cgaaccggta gcaagcctgg tccgtgaatg cccgaaatgg
     9901 gatggattac cgctcacact ggatgtcagc gccacttttc cggagggagc cgcagtccgg
     9961 gattattaca gccagcaaat tgcgatagtg aagaacggtc aaataacgtt acaacccgct
    10021 gccaccagca acggtttact cctgctggaa cgggcagaaa ctgacgcccc tgcccctttc
    10081 gactggcata acgccacggt ttactttgtg ctgacagatc gtttcgaaaa cggcgatccc
    10141 agtaatgacc agagttacgg acgtcataaa gacggtatgg cagaaattgg cacttttcac
    10201 ggcggcgatt tacgcggcct gaccaacaaa ctggattacc tccagcagct gggcgtcaat
    10261 gctttatgga taagtgcccc atttgagcaa attcacggct gggtcggcgg cggtacaaaa
    10321 ggcgatttcc cgcattatgc ctaccacggt tattacacac aggactggac gaatcttgat
    10381 gccaatatgg gcagtgaagc cgatctacgg acactggttg atagcgcgca tcagcgcggt
    10441 attcgtattc tctttgatgt cgtgatgaac cacaccggct atgccacact ggcggatatg
    10501 caggagtatc agtttggcgc gttatatctt tctggtgacg aagtgaaaaa aacgttgggt
    10561 gaacgctgga gcgactggaa acctgccgcc gggcaaacct ggcatagctt taacgattac
    10621 attaatttca gcgacaaaac aggctgggat aaatggtggg gaaaaaactg gatcagaacc
    10681 gatatcggcg attacgacaa tccgggattc gacgatctca ctatgtcgct ggcctttttg
    10741 ccggatatca aaaccgaatc aacgaccgct tctggtctgc cggtgttcta taaaaacaaa
    10801 acggataccc acgctaaagt catcgacggc tttacccctc gcgattactt aacccactgg
    10861 ttaagtcagt gggtccgcga ctatgggatt gatggttttc gggtcgacac cgccaaacat
    10921 gttgagttgc ccgcctggca gcaactgaaa accgaagcca gcgccgcgct tcgcgaatgg
    10981 aaaaaagcta accccgacaa agcattagat gacaaacctt tctggatgac cggtgaagcc
    11041 tggggccacg gcgtgatgca aagtgactac tatcgccacg gcttcgatgc gatgatcaat
    11101 ttcgattatc aggagcaggc ggctaaagca gtcgattgtc tggcgcagat ggatacgacc
    11161 tggcagcaaa tggcggagaa attgcaggat ttcaacgtgt tgagctacct ctcgtcgcat
    11221 gatacccgcc tgttccgtga agggggcgac aaagcagcag agttattatt attagcgcca
    11281 ggcgcggtac aaatctttta tggtgatgaa tcctcgcgtc cgttcggccc caccggttcg
    11341 gatccgctgc aaggtacgcg ttcggatatg aactggcagg atgttagcgg taaatctgcc
    11401 gccagcgtcg cgcactggca gaaaatcagc cagttccgcg cccgccatcc cgcaattggc
    11461 gcgggcaaac aaacgacact ttcgctgaag cagggctacg gctttgttcg tgagcatggc
    11521 gacgataaag tgctggtcat ctgggctggg caacagtgac ttttccggct tcccgttcgt
    11581 cagtacctcg ggaagccgcc aaccaggata aaatgtcaga gctaatcagc gttgcaggat
    11641 aaagcaccgc tcactcttca acagagcgat ttgcacccca gcaaatgtag cgttattgtt
    11701 accttccttg ctacagagtt cgacagatat cccgctatga cattctccct ttttggtgac
    11761 aaatttaccc gccactccgg cattacgctg ctgatggaag atctgaacga cggtttacgc
    11821 acgcctggcg cgattatgct cggcggcggt aatccagcgc agatcccgga aatgcaggac
    11881 tacttccaga cgctactgac cgacatgctg gaaagtggca aagcgactga tgcactgtgc
    11941 aattacgacg gtccacaggg gaaaacggag ctactcacac tgcttgccgg aatgctgcgc
    12001 gataagttgg gttgggatat cgaaccacag aatattgcac taacaaacgg cagccagagc
    12061 gcgtttttct acttatttaa tctgttcgcc ggacgccgtg ccgatggtcg ggtcaaaaaa
    12121 gtgctgtttc cgctggcacc ggagtacatt ggctacgctg acgccggact ggaagaagat
    12181 ctgtttgtct ctgcgcgtcc gaatattgaa ctgctgccgg aaggccagtt taaataccac
    12241 gtcgattttg agcatctgca tattggcgaa gaaaccggga tgatttgcgt ctcccggccg
    12301 acgaatccaa caggcaatgt gattactgac gaagagttgc tgaagcttga cgcgctggcg
    12361 aatcaacatg gcattccgct ggtgattgat aacgcttatg gcgtcccgtt cccgggtatc
    12421 atcttcagcg aagcgcgccc gctgtggaat ccgaatatcg tactgtgcat gagtctttcc
    12481 aagctgggtc tacctggctc ccgctgcggc attatcatcg ccaatgaaaa aatcatcacc
    12541 gccatcacca atatgaacgg cattatcagc ctggcacctg gcggtattgg tccggcgatg
    12601 atgtgtgaaa tgattaagcg taacgatctg ctgcgcctgt ctgaaacagt catcaaaccg
    12661 ttttactacc agcgtgttca ggaaactatc gccatcattc gccgctattt accggaagat
    12721 cgctgcctga ttcataaacc ggaaggagcc attttcctct ggctatggtt taaggatttg
    12781 cccattacga ccgagcagct ctatcagcgc ctgaaagcac gcggcgtgct gatggtgccg
    12841 gggcacaact tcttcccagg gctggataaa ccgtggccac atacacatca atgtatgcgc
    12901 atgaactacg tgccggagcc ggataaaatt gaggcgggtg tgaagattct ggcagaagag
    12961 atagaaagag cctgggctga aagtcactaa aaattgcctg atgcgctacg cttatcaggc
    13021 ctacatgatt tctgcaatat attgaatttg gtagaatttg taggccggat aaggcgtttt
    13081 agccgcatcc ggcataaaca aagcgcacta ttccggcgtt gagaaacgcc ggaaaacgtt
    13141 tcaaacaggc tgctgccgta gccgatgccg ctgcactttc tcgacatcca tgcactgcaa
    13201 cgcctttgtc gggcaggctt caacacaggc cggccccgtc tcccgatgcc agcacagatc
    13261 gcacttaatc gccctcgcct ttttgcgcga agaaacgacc tccatcgcac caaacgggca
    13321 agccagcata cagcttttac agccaatgca acgtgattgt tcaacgaaaa tatgcccatg
    13381 ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt cacactgatg
    13441 acaggctacc gccgtggtcc agctgtggtc tttaatgaca cgaatacggg aaataaactc
    13501 gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc aagccacttc
    13561 acaggtacgg caaccgatac atttcgtcgc atccgcaata ataaaccggt tcatgatcac
    13621 tcctttgcac taaaacagca gagtgccaga gagcgcgtct ctactgcttt gatccaacga
    13681 aggaaatgtt aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc ctggtcgtcc
    13741 ctgacagtaa atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg
    13801 agatttttct cccccacctg tttcagacgt gatgtcgaaa gagaaatcga cacggcgtac
    13861 ggcacccgcc catgaatatc aaacaccgga acagcaatac aggagacgcc gagttcgttt
    13921 tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg
    13981 ggcagctcgg taatggtatt gcgggttaac ggctggatct catgctgatg gcttttccag
    14041 tatgacttca cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat tgcggaacag
    14101 tagagcggca tatgctggcc tatataggca cgggttcgca gcatcccggt tgtgggttcc
    14161 agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat ggtttcacca
    14221 gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagac
    14281 agcgccttct gcccgacggc aataaatttg gtagtcaggc ggtaactccc tgcggcgggc
    14341 gcggtggtca catagccgca ggactgtaat ccctgcaata agcgatggac ggtactctta
    14401 tttaaaccag ccagctccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa
    14461 atctcaatca gcatcaaccc gcgaaacaga ctctggcttc cggctggacg ctctttttcc
    14521 tgcgccatct cgttctcttt ttttcccatc acttctttcc ccattttgtc gcgtcctgat
    14581 ggtagcgcaa agtgtgtcgt agttcacgat ctcgacagat aatttataac cacttgattt
    14641 ttaagttttt tgaaattcat caatcagatt gctttgttaa aaagcgatcg atatatttga
    14701 aatcagattt cgcatattga aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc
    14761 agggatgaaa gtgacatttg agcagttaaa agcagccttt aatcgagtct taatttcacg
    14821 cggcgttgac agcgaaacgg ctgatgcctg tgccgagatg ttcgcccgca ccaccgaatc
    14881 cggcgtttat tctcacggcg tcaaccgttt ccctcgtttc attcaacaac tggaaaacgg
    14941 cgatatcatt cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg
    15001 ggacgcccag cgttcgatcg gtaacctgac agcgaaaaag atgatggatc gcgccatcga
    15061 actggctgcc gatcatggta ttggtctggt ggcactacgt aatgccaacc actggatgcg
    15121 cggcggcagc tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa
    15181 ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt cgcatcggca ccaacccgct
    15241 gatcgtcgcc attccttcta ccccaatcac catggtcgat atgtcgatgt cgatgttctc
    15301 ttacggcatg ttagaagtta accgtctggc aggtcgtcag ctcccggttg atggtggctt
    15361 tgatgatgag ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt
    15421 gccgatgggc tactggaaag gttctggcat gtcgattgtg ctggatatga tcgccactct
    15481 cctttccgac ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcgt
    15541 ttcgcaaatc tttatcgcca tcgaagtgga taaattgatc gacggcccca cccgcgatgc
    15601 aaagctgcaa cgcatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc
    15661 catccgctta cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat
    15721 caccgttgat gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt
    15781 tttggacata tcgcgcaacc caatccgtgc cgtttgcctg ccgccattga aaaggcgctc
    15841 gattttctgc gcgctaccga tttcaacgcc ctggagccgg gtgttgtcga aatcgacggc
    15901 aagaatattt atgcgcaaat t
//
LOCUS       DARNVM010000111        15785 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.111, whole genome shotgun sequence.
ACCESSION   DARNVM010000111 DARNVM010000000
VERSION     DARNVM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.111"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1692
                     /locus_tag="SI621_003208"
     CDS             <1..1692
                     /locus_tag="SI621_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HEI4065883.1"
                     /translation="TPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADEDVR
                     KELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPGSSR
                     QAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDLWEG
                     SLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSLQSH
                     ITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSGKRK
                     EQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRREAL
                     ELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDE
                     GFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLG
                     WENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFRE
                     KVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            1689..2594
                     /locus_tag="SI621_003209"
     CDS             1689..2594
                     /locus_tag="SI621_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEI4065884.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLAGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2591..3661
                     /locus_tag="SI621_003210"
     CDS             2591..3661
                     /locus_tag="SI621_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEI4065885.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            3797..4480
                     /locus_tag="SI621_003211"
     CDS             3797..4480
                     /locus_tag="SI621_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065886.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            4496..4906
                     /locus_tag="SI621_003212"
     CDS             4496..4906
                     /locus_tag="SI621_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065887.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            5127..5948
                     /locus_tag="SI621_003213"
     CDS             5127..5948
                     /locus_tag="SI621_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HEI4065888.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGGINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            6024..6509
                     /locus_tag="SI621_003214"
     CDS             6024..6509
                     /locus_tag="SI621_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HEI4065889.1"
                     /translation="MKTVSHNATTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQYF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGITACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            6525..7001
                     /locus_tag="SI621_003215"
     CDS             6525..7001
                     /locus_tag="SI621_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HEI4065890.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            7064..7285
                     /locus_tag="SI621_003216"
     CDS             7064..7285
                     /locus_tag="SI621_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HEI4065891.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            7422..7727
                     /locus_tag="SI621_003217"
     CDS             7422..7727
                     /locus_tag="SI621_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HEI4065892.1"
                     /translation="MPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQA
                     FPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN"
     gene            7816..8189
                     /gene="cbtA"
                     /locus_tag="SI621_003218"
                     /pseudo
     CDS             7816..8189
                     /gene="cbtA"
                     /locus_tag="SI621_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            8186..8380
                     /locus_tag="SI621_003219"
     CDS             8186..8380
                     /locus_tag="SI621_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065893.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            8393..8506
                     /locus_tag="SI621_003220"
     CDS             8393..8506
                     /locus_tag="SI621_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HEI4065894.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(8795..8941)
                     /locus_tag="SI621_003221"
                     /pseudo
     CDS             complement(8795..8941)
                     /locus_tag="SI621_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            8995..9177
                     /locus_tag="SI621_003222"
     CDS             8995..9177
                     /locus_tag="SI621_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HEI4065895.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(9278..9607)
                     /locus_tag="SI621_003223"
     CDS             complement(9278..9607)
                     /locus_tag="SI621_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HEI4065896.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9779..10837)
                     /locus_tag="SI621_003224"
     CDS             complement(9779..10837)
                     /locus_tag="SI621_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEI4065897.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRLLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(11035..11508)
                     /gene="sbmC"
                     /locus_tag="SI621_003225"
     CDS             complement(11035..11508)
                     /gene="sbmC"
                     /locus_tag="SI621_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HEI4065898.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(11627..12793)
                     /gene="dacD"
                     /locus_tag="SI621_003226"
     CDS             complement(11627..12793)
                     /gene="dacD"
                     /locus_tag="SI621_003226"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HEI4065899.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            13002..14429
                     /gene="sbcB"
                     /locus_tag="SI621_003227"
     CDS             13002..14429
                     /gene="sbcB"
                     /locus_tag="SI621_003227"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEI4065900.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAQEIV"
     gene            complement(14472..14699)
                     /locus_tag="SI621_003228"
     CDS             complement(14472..14699)
                     /locus_tag="SI621_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEI4065901.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(14713..15771)
                     /gene="yeeE"
                     /locus_tag="SI621_003229"
     CDS             complement(14713..15771)
                     /gene="yeeE"
                     /locus_tag="SI621_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HEI4065902.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYVAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         4122 a         4027 c         3940 g         3696 t
ORIGIN      
        1 acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg ggatcaactg
       61 cagaaagcct ctgcggttct ggctgtgatg gattacaccc agatgaactc aaaagcggat
      121 gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg cctgtttgta
      181 ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc cgtacgccag
      241 aaagttccgg caatgctgaa cagcgatgtg ctgcccgcct cccgcgttta tcccggatcc
      301 tcacgccagg catacctggc taaccgtgcg cttcatgagt tacggaaaaa cggaaccctt
      361 cctgttgatg aagcctgggt cgatgatttt atcagaggtg ccttcggttg catgaaaaaa
      421 gaatacgtct gtaaagacag tgaactggca actgaagggg cgacagacct gtgggaaggc
      481 tcacttatcg atcaactgat aacggaagtc atacagagct cacattccag agctgcggca
      541 ctggcggttg actctgccgc cgcaaaactg atgcagaatg cagaaaatat cagtgaatac
      601 ctgttgttac gccatcaggg gctacagcaa agcattcagt cactgcagtc gcatatcacc
      661 agcctgcttg cggatatccg ggaaatcgcg gactgtcagg agcagatgac cactgatgtc
      721 agaatggcca tggaggagat cgatactaaa acccgggaat tactgacggg ggtctgcacc
      781 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
      841 ctggaggaag aagacgcaga acaacgaagg tctcagtccg gattatgggg gaaaatttct
      901 caatggtccg gtatcaacaa ccagggccgg gaggattaca ggaaacgaga ttttgccccg
      961 gacagccccg aaataaaatt cagtgatcgc agggaagccc ttgaactgat gacgcaaatc
     1021 gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac agttccggcc tgagctggag
     1081 aaaatcgtca gcgggattga aacaggtttt cgtggcacgg ccctgtacgc cacagaaaac
     1141 attgccggtc gcatcaatgc ccgcctggag gatgagggct ttaccgtaaa aatcagtttt
     1201 ccggcagtca gccagttaca gacccggctc gcggtaaaaa taaatctgag tgcgcttatg
     1261 gaggaaagaa cggagaccgt cacccgtcgc cgtcggcaga gtggcgtatg gggaaccgtt
     1321 tgtcgatggt ttggcaccag tgacctgggc tgggaaaact atgacgagga tgtgagtcgc
     1381 agcgtgatca atatcaacaa ggtcagagag gaagttatgt cactgacccg ggcatatttc
     1441 ggggagctgc aggcatccat tgagcaggat attaaccagc ccgtccgcca ggaaatcgat
     1501 gcctttttct gcgcattcag ggagaaagtt gaacaactgc gtaacacgct gattcagagc
     1561 tctgaagatc ataaacgcga tcagcaggcg caggaacggc ttaccgggcg acttcaggca
     1621 ttaaatgaaa gggttcctga gctcattact gacagtaagg cgctgaggga agagctggag
     1681 acaatgctgt gacttcacca tttattcaac aaattgctga taaccgggta tgtcaggtac
     1741 tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa cggaatcgat gttgcacagg
     1801 agcatatccg cacggccgga gagcgcacgt tcttccggcg tttaaaagag gggctgaccg
     1861 gcgaaggcgc tgcacggcag aatgccatca atgcgtcgct tgcacagggc gttgaggcgt
     1921 ccctgcgctg gctgacggag atgacaacgt cacttgccac cacaaactac gcgattaccc
     1981 gggtaaacga cagggtcagt tcactggtca gtgatacagc caggctggcg cattattccg
     2041 cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt tcaccataaa ctgaatcatc
     2101 ttgaagaaaa actccaccgt gttgaccagg ttcagcgggc gcagttacat cttgagcaga
     2161 tattctcatg gtggagcgcc gggcgatatg cgtcattttc ccctgccgga cgttgttatg
     2221 tggcacttga agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag ggcgaaacag
     2281 gccaggttaa ccagctactg gatattctca gacataaagc attaacgcag atggcacagg
     2341 agagtggcgg tagtgcaacc gtgcgtctga acactctgga ctggcttgct ggtcagggcc
     2401 gggagcaggc agataacgag tggcatgacg cgattaactg gctgggagac tggtgcagtg
     2461 aagagcagca tccggtgatc tggtcaacca cgcaggctgc agaacactta ccggttcgca
     2521 tgcctcgcct ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa atatttcaga
     2581 agggggctgc atgagtacag aaatgaaaac ggggctggtg ctgtccggtg gcggagcggt
     2641 gggcgcttat caggcgggag tggttaaggc actggcagag tgtggtacac agatcagcat
     2701 ggtttcaggg gccagcattg gcgcattcaa tggtgccatt atcgcggcct ctaccgatct
     2761 gtcagaagct gccgtacgcc tggaggcgct ctgggatcat ctggggaata atcaggtgct
     2821 gtcggtaaac agattggttt acttttcatt gctgaaaaaa ttgttccagg caatgaacct
     2881 ctgccagatc cccggacgtg caggagcact gcttacgacg cttcttcgcc atatatcgac
     2941 aatcaacggg tttgacaatc tgatggctca gccgttgttg tcagatgagc ccctgacagc
     3001 gctgatggat cattatcttg atactgatgc tctggcagac gggctaccgc tgtatgtgtc
     3061 gctgtacccc acagaagggg gcatgcagga tattattgac tgcattcgtg ctgaactggg
     3121 tgtcggaacc acgaaaaacg ccgtttttca gcatatccag agcctgcccc gcggacagca
     3181 gaaagaggct ctgcttgcgt cagccgcgct gcccctgctg ttccgtcccc gtgaggttca
     3241 ggggacaatg ttcggtgatg gtggtatggg aggatggcga aatatgcagg gaaatacccc
     3301 tgtgacgcct ctggtcgatg ccggatgcaa tatggtgatt gtgacgcatc tgagtgacgg
     3361 ttctttatgg gatcgccagg cttttccgga caccacaatc cttgagatcc gtccccggaa
     3421 aaggctgaaa tatgcaggtg atggtggcaa cagcggcggt ctgctcagtt ttacatcggc
     3481 acataccgac gcctggcgtc agcagggcta tgaagacacg atgctggcga tggagcatat
     3541 ccggaaaccg ctggcagcac gtcaggcact gacccggtca gaggcggtat tgcagaaaag
     3601 cctggatata acggaagagg cagatttggc actgagaaac gcgatggccc ggattaaata
     3661 aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt ttcctgtctg gtgttattga
     3721 aaatattttc agatatatat tacctgagag aaagttttac ccctcacctg tcaattcact
     3781 cactggaaat ttaattatga aattacctgt aaagttatta atgagcctta tatctctggt
     3841 cagcgttatt gcacgtgccg gggaatataa aaattacagc cgggatgaaa taaaatactg
     3901 gcgatataca tcatacaagg gggggaaact tccggaaggt ttcactgatg agaaattttc
     3961 cagcgccatt tacaacggaa gaatatttac aatgaaacgt ttacataccc ttatgttatt
     4021 tctggcggtt ctgttcacgg gctttaactt taacgcagaa gcggctaccg taaaacaagc
     4081 tcttagttgt aacccggaag cctgggctga acaacctgga gcgtgtccat caacgtacga
     4141 gttgtacgaa ggtgacgcta cctacaaagc cgctatcgac aaagcattaa aaccggtcgg
     4201 actgagcggc atgttcggta aaggcggcta tatggatggc cctggtggag gtatcacgcc
     4261 agtaaacatt aacggtacag tctggttcca gggcgacggt tgcaaagcca atacctgcgg
     4321 ctgggacttt atcgtaacac tctataaccc aaaaacccat gaagttgttg gctaccgtta
     4381 ctttggttta gatgacccgg cctacctggt ttggttcggt gaaattggcg tgcatgaatt
     4441 cgcgtatctg gtgaaaaact acgtagctgc ggttaactaa tttaaggagt ctatcatgaa
     4501 aacttttatc aaaactttac tcgttgctgt aactattctg ttctctgtct tcgctactgc
     4561 gaaacaagta aaactgccaa ataacatcaa atacgttaat actacagaag cgttttcctg
     4621 taccgaaatt gacggtatga attgccagac aaagaatcag ttcaactata aagataacag
     4681 ctatgttttc gtgcttgaac gtggtggtgc ctggtgctac gactacactg tctcggtagt
     4741 taacctgaaa accgggaaag cacaaatgat tgaatacggg gacaatcaac tgtgctcagg
     4801 tagcaacaaa ccgttcttcg aaatcaaaaa tggcgtaccg acggtaggag tcatcgacac
     4861 atccggaaaa cctgtcgttg tagcccagga caaacttaaa atctaaggag taaaccatgc
     4921 gtttctctgc acattattaa tatcaaaggt aaatacagcc ccagccattg ttaccggaaa
     4981 gccgcttaat gcgtggctcg ccagtgaaag gcctctcaga caataaacct tattcatttc
     5041 acccgtcagg ccgcgtcttc tccgggagac gcggcttttt tcatttattg caccagtaaa
     5101 tcttaaccac cgataaggag caaagtatgc gattagccag tcgttttggt tatgctgcaa
     5161 accagatacg ccgtgaccgt ccgctgacac atgaagaact gatacgccat gtacccagta
     5221 tttttgggga agaccggcac acctcccgca gtgaacggta tgcgtacatt cccaccatta
     5281 ccgtcctgga aaatctgcag cgggaaggct ttcagccgtt cttcgcctgc cagacccgtg
     5341 tgcgcgacca gagccgccgg gaatatacca aacacatgct gcgtctgcgg cgggccggag
     5401 ggataaacgg agaacatgtc cctgaaatta ttctgctcaa ctctcatgac ggtacctcca
     5461 gctaccagat gctgccgggt tacttcaggt tcgtctgcca gaacggctgc gtctgtggtc
     5521 agtctctggg ggaagtgcgt gttccacacc ggggaaatgt agtggagaaa gttatcgaag
     5581 gggcttacga ggtggtgggc gtgtttgacc ggatagagga gaagcgtgat gccatgcagt
     5641 cgctggtcct gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt
     5701 atggtgacga acatcagccc gtcaccaccg ccgacattct gacgccgcga cgccgggagg
     5761 attacggtaa ggacctgtgg agcgcatatc agaccatcca ggagaatatg ctgaaaggcg
     5821 gaatttctgg tcgcagcgct aaaggaaaac gtatccacac ccgtgccatt cacagcatcg
     5881 acacggacat taagctcaac cgggcgttgt gggtgatggc agaaacgatg ctggagagcc
     5941 tgcgctgatg ccgtttccct gagggaatca ttcagctttc gttcctgaaa aacacattta
     6001 tctgttattt aaggagttat ctcatgaaaa ccgtttctca taatgccact acaccttctg
     6061 tttccgtgac cactgcatca gggaataatc cgcctcagct ggttgccact cccgtccctg
     6121 atgaacagcg catcagcttc tggccgcagt attttggcct cattccacag tgggtgaccc
     6181 tggagccccg tgtcttcggc tggatggacc gtctgtgcga agactactgc ggtggtatct
     6241 ggaatctgta caccctgaac aacggcgggg catttatggc acccgaaccg gatgatgatg
     6301 atgacgaaac atgggtactg ttcaatgcca tgaacggtaa ccgcgcagaa atgagcccgg
     6361 aagccgccgg tattaccgcc tgtctgatga cgtacagtca tcatgcctgt cgtacggaga
     6421 attatgccat gacggtccat tattaccggt tgcgggatta cgccctgcag catccggaat
     6481 gcagcgccat tatgcgcatc atcgactgaa tggagagaag cacaatgcaa caactttcct
     6541 ttctgcccgg agagatgacg cccggcgagc gcagcctcat tcaacgggcc ctgaaaaccc
     6601 tagaccgcca tcttcatgaa cccggcgtgg ccttcacctc cacccgtgca gcacgggaat
     6661 ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg tatctgaaca
     6721 accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag
     6781 tccatccccg ggaagttatt aaacgcgccc tgtaccacaa tgccgctgcc gtggtactgg
     6841 cacacaatca cccgtccggt gaagtcacac ccagtaaggc agaccggctt atcaccgaac
     6901 gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg atagtcggtg
     6961 gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccttcaca accacatcac
     7021 acctgttttc acttttatct tctgtcttca gaggtatccc attatgaaaa ttatcacccg
     7081 tggtgaagcc atgcgtattc accaacaaca tccgacatcc cgtctttttc cgttctgtac
     7141 cggtaaatac cgctggcacg gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat
     7201 tcccggtgtg ctggccgtgt ttgctgaacg ccgtaaggac agttttggtc cgtatgtccg
     7261 gctgatgagc gtcaccctga actgagtggg aattctgatg agcagaatta tcgccactac
     7321 cggaccattc ttagccgatt ttctgtaagg attttatcgt gtcagacaca ctccccggga
     7381 caacacttcc cgacgacaat cacgaccgcc cctagtgggg gctgccctgc accgtgacgc
     7441 cctgtttcgg ggcacgtctg gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg
     7501 ccggtatcag aggcctgttc agcgatgcag atgcgtacca cctggaccag gcctttccgc
     7561 tgctgatgaa acaactggaa ctcatgctca ccagcggtga actgaatccc cgccatcagc
     7621 ataccgtcac gctgtatgca aaagggctga cctgcaaagc cgataccctc agcagttgtg
     7681 gttacgttta tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag
     7741 ccttctgcta cggcctggtg ttttcaccac gccacttttc catttttata tctgcatatc
     7801 aggaaaatct tcagtatgaa aacattacct gtattacccg ggcaggcggc cagttctcgc
     7861 ccgtctcctg ttgaaatctg gcagatactg ctgtcccgac tgctggacca gcactatggc
     7921 ctcacacaga atgacacacc ttttgccgat gaacgtgtga ttgagcagca tattgaggca
     7981 ggcatttcac tgtgtgatgc ggtgaacttt ctcgtggaaa aatacgcgct ggtgctaccg
     8041 accagccggg attcagcgcc tgtacccgct ctcagttaat aaacagcatc gatatcctcc
     8101 gggctcgcag ggcgaccggc ctgatgaccc gcgacaatta cagaacggta aataacatta
     8161 ccctgggtaa gtatccggag gcgaaatgat gacactggaa gccgacagcg ttaacgtaca
     8221 ggcgctggat atggggcaca ttgtcgttga cattgatggt gttaatatca ctgaactgat
     8281 taataaggcc gctgaaaacg gttattcact ccgcgtggtg gatgaccgtg actctaccga
     8341 aacaccggca acttatgcca gccctcacca gttgctgtaa gacaatgcag tgatgcaggg
     8401 tatagtgcca ttaaccacag aaacagcact gttgtcctgt attaagcagg caagagaaga
     8461 tgtccttacc ctgcgccatc tgcagcttct gcaccagaac aggtgaatct cactcaacag
     8521 gcttcatttc ccctgacgcc agcctgaata cagctggcgt tttcatttat aaacagaaag
     8581 gaaaaccgat aataatggaa tggacgcccc gaccatgaag caacggtagg atattactga
     8641 taaaccttcc agtggaggtg cgttatggcc gttttaacga ttagcattga cctgacaaaa
     8701 aatgaattcc aaatccacgg tctgggtagg aacagaaaaa tctaagctca gaaaacggat
     8761 taaagtaagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
     8821 atggcaggag ttcgtcaaca cggttggagg gccattccgg cagtatgctc agaatatggc
     8881 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
     8941 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
     9001 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
     9061 gtgaaatgtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcggttaat
     9121 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ccgctaattt
     9181 cgataggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
     9241 agggttgccg ggcaaccctc attgaataaa acaggaatta cttcgcttcg ccgtttttca
     9301 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
     9361 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
     9421 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     9481 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     9541 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     9601 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     9661 atagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     9721 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     9781 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgc gccgtttcca
     9841 tgttcagcca cacataacca tagattggtg tttccacaac cttcaggtca tggtggttat
     9901 taagcaacag acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     9961 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
    10021 gctgggtatc acgaagtttt tgcgcgttca ataacacgaa atggctgggg cgcgtggccc
    10081 agtatgcatt gatttgcaac tccagcatac aaaccagatt gcggttaatg gtctggatac
    10141 cttcatatat cgattttgga atacgggttt ctttgctggc gggcgcaatc agcccacgca
    10201 ttttcacggc atcggtcagc agtttttgta gatggctttc cagacgtggg cgttcgagta
    10261 agttcggtga gaatgcagat tgatagaccc gattatactc ggtcagactt ttcgccagtt
    10321 gaatgcgcca gtggatgaac gcccgttgtg gccagatacc ggtaaacaac attgccagta
    10381 aagagccgag gatcacatcg ccgcttcgcc ataacgccgt atcaatttca cctgtcgggg
    10441 aacccacaac aattgccagc gtcaccccaa tcaataatcc ttgatacggt ttcttgccca
    10501 gcgccagcca accgcaaaga aacatcgccg ccgcgcacca gactaacatc agcggtaacg
    10561 aaattaactc cagttgcaga gcgataagac ctaaaatcga acccaacacc gtaccgccaa
    10621 tacgttcgaa ggcacgaggg acaacgttcc cccagaacga gattggcccc ataatcacca
    10681 ccatggtgac cagcggccag gtgctttccg ggatagtaaa caggcggata atgagaaaag
    10741 tgagcaggaa tgccagcgcg acccgagtac catgcacaat gcggtaatgg cggtataccc
    10801 ggatttcaaa cgggcttaat gacttatcgg cacgcacgca gaaactccgt aatgaagatt
    10861 aaaagcgctc ataacacatc tgtcggccag aaggcgcgcc tctgagttat ccgacggaca
    10921 taccgggaac agtgtaattt cccggcagga gcgaaaaatt tgaaaggcgc atcttactct
    10981 tttcgctttc aaaaaaagag tggtcttcgc gtcaacacac cgccctgaga tgaattagtg
    11041 atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt ccgcgccatt
    11101 gttcaagtaa acctcgaagc agggctttgg taacatttca taggcactgt cctgcaagag
    11161 gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac gagccaccgc
    11221 caccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt tttcggggag
    11281 cgtaaagtta ttcggtaccg tcacgacggt gtcgcagcgt aatttttcgg cgggtgtttc
    11341 atccggattg tcgtaataga cagcaaccca ctccttcggc acaatatttt tgctatctat
    11401 ccacatcatc aactgctcaa aacctttctt taccgtctgc tcccacgggc caacaagatg
    11461 gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga cgcctccact
    11521 aatactgttt ttatatacag tatagttgca atattaaaac cacaaggaat gagtgttgat
    11581 tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag gccttatggt
    11641 ggaaataatc actcagccgg gaaaacatgc tgccttcccc gacagattcc agggtaacca
    11701 gcggccagtg cgccacctgt ttatcacggt cataaagttc aatttcccct acccgctgat
    11761 gggcgctaat tggcgcggtg agttctttac catcaagggt atatttggct ttgatatgtg
    11821 gaatttcggc tttcggtagc accatccaga actcttgttc cgttcccagg gcgatatttt
    11881 ctttatcgcc ataccagatg cgttccgttc cgaccttttt cccacggtgc aaaatttgca
    11941 ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc tcacgacctt
    12001 ttgcactgtc agcccccatt accactgcaa tgagacgacg ctgcccatcc acagccgaag
    12061 caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca acattcatgg
    12121 ttttatccca caataacccg ttacggtttt gctgagtgat accgttccag gtgaggcttt
    12181 tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga gaaagcacag
    12241 ctaaatcata agccgagcta tgctggccag gtgcatccag accatgcact gtttcaaaat
    12301 gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca aactgccgtt
    12361 gcccaccggc aatatagtca gccagagcaa cacaagcgtc atttccggaa tccacaatta
    12421 aaccacggct taaatcacgt accgatacgc gatcgccctc tttcagaaac atcagtgaag
    12481 aaccgacaaa caccggatta tctttcgccc acgcatcgcg ccccacggtg acaatatcgt
    12541 ctggcgtaat gcgatgacta tcgatagcac gatccacgac atagcccgtc atcagctttg
    12601 tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgagaatc tgaccggtgg
    12661 tgtaatccat caatacccag gacccggcat gaatctctgg aggctgaggt gaaaaaggaa
    12721 tgttttccgc cgcaaaacca gacgataaat taaaaacgaa caaagaagca gcaataataa
    12781 gacggcgttt caacagcaaa ccctcaggtg tttcaaatag ctgttctttt tacggaaata
    12841 cttatgaact ggctggaata aagtgcaaga aaatgtgact actacctcat ttttatctga
    12901 catgatctgt tgccactcgc tgccaaattg tcgcgctaaa gctgattagc acggtgatat
    12961 ttgatactct ggcagacagc agaaataacg gatttaacct aatgatgaat gacggtaagc
    13021 aacaatctac ctttttgttt cacgattacg aaacctttgg aacgcacccc gcgttagatc
    13081 gccctgcaca gttcgcagcc attcgcactg ataacgaatt caatgtcatc ggcgaacccg
    13141 aagtctttta ctgcaagccc gcggatgact atttacccca gcctggagca gtattaatta
    13201 ccggtattac cccgcaggaa gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc
    13261 gtattcactc gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt
    13321 tcgacgatga agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga
    13381 gctggcagca tgataactcg cgctgggatt tactggatgt tatgcgtgcc tgttatgccc
    13441 tgcgcccgga aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg
    13501 agcatttaac caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg
    13561 tgtacgccac tattgcgatg gcgaaactgg taaaaacgcg tcagccacgc ctgtttgatt
    13621 atctctttac ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga
    13681 aacccctggt tcacgtttcc ggaatgtttg gggcatggcg tggcaatacc agctgggtgg
    13741 caccgctggc gtggcatcct gaaaatcgca atgccgtaat tatggtggat ttggcaggag
    13801 acatttcgcc attactggaa ctggatagcg acacattgcg cgagcgttta tataccgcaa
    13861 aaaccgatct tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc
    13921 cggtactggc ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc
    13981 gtcagcattg cctcgataac ctaaaaattc tgcgtgaaaa tccgcaagtg cgtgaaaaag
    14041 tggtggcgat attcgcggaa gccgaaccgt ttacgccttc agataacgtg gatgcacagc
    14101 tttataatgg ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg
    14161 agccgcgtaa tttaccggca ctggatatca cttttgttga taaacgaatt gaaaaactgt
    14221 tgttcaatta tcgagcgcgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct
    14281 ggcttgagca ccgccgccag gtcttcacgc cagagttttt gcagggctac gctgatgaat
    14341 tgcagatgct ggcacaacag tatgccgatg acaaagagaa agtggcgctg ttaaaagcgc
    14401 tttggcagta cgcgcaagag attgtctaat gtgaagcctc cgccgctggt acggcggagg
    14461 acaatgttga gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc aatctgctga
    14521 taatcggtga tggcatgtcc ttcttctgcc gcccactgtg ggattgcttc agttgcctgg
    14581 gtgcaatcaa actcaattac caactcatcg ccgctggcca tttctgccag tgctgctttt
    14641 gcttctatga gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc
    14701 tttacacctt acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac
    14761 atacacaagc caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc
    14821 ctgccaggtc atcattgcag tcatgaccag tccattaccg atagagcaac cacctgcaat
    14881 gctggcaccg aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc
    14941 agcagcgcga acgcggaact cacggctcgc tttggcggca ataaatgacc ccacgaagat
    15001 ccctaacacc aggaaaacgc cccagttaat gtatttcacg tcacccgcga ccaaaaattg
    15061 cagaatattg gccgttgggg aagtgatgcc taacccaaac atacgtccgg ttgcttcact
    15121 caggggccag gctaaaagcg caatcaaacc gatgagtaca gctgtgacaa agggatgcca
    15181 gcgtttttca aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt
    15241 gagttttggc ttcttcaact ctttcatcac cacccagagc gttatcacca gcaaaacggc
    15301 aaccaacggc cacacagaca aattaaaagt atcagcaata gagttatgtt cagtagtgta
    15361 gtgctgcaag gtttgattta aaccactggc gtgtggagaa cgcatcaccg cactcatcac
    15421 catataagtg aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt
    15481 cccggtggca catccacccg ccagaacaat tcccagcccg aaaagatacc cacctataac
    15541 agtaccaagc cacgggaacg ccccggcttc gtaagtcagt aggcccgcct gaattaacgc
    15601 aaaaaccccc acgctttgta ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt
    15661 tttcgcgaca tacatatcgc gaaaaccacc tgtcaggcaa aaacgcccac gctgcatgac
    15721 aaatcccagc agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt
    15781 aacca
//
LOCUS       DARNVM010000112        15671 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.112, whole genome shotgun sequence.
ACCESSION   DARNVM010000112 DARNVM010000000
VERSION     DARNVM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.112"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            192..815
                     /gene="nfeR"
                     /locus_tag="SI621_003230"
                     /gene_synonym="yqjI"
     CDS             192..815
                     /gene="nfeR"
                     /locus_tag="SI621_003230"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HEI4065903.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(844..2364)
                     /gene="aer"
                     /locus_tag="SI621_003231"
     CDS             complement(844..2364)
                     /gene="aer"
                     /locus_tag="SI621_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HEI4065904.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2782..4161
                     /gene="ygjG"
                     /locus_tag="SI621_003232"
     CDS             2782..4161
                     /gene="ygjG"
                     /locus_tag="SI621_003232"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HEI4065905.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFDALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4203..4535)
                     /locus_tag="SI621_003233"
     CDS             complement(4203..4535)
                     /locus_tag="SI621_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEI4065906.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4754..5737
                     /gene="ebgR"
                     /locus_tag="SI621_003234"
     CDS             4754..5737
                     /gene="ebgR"
                     /locus_tag="SI621_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HEI4065907.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPSSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5921..9013
                     /gene="ebgA"
                     /locus_tag="SI621_003235"
     CDS             5921..9013
                     /gene="ebgA"
                     /locus_tag="SI621_003235"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HEI4065908.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISTMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERLVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVIPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPISGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9010..9459
                     /locus_tag="SI621_003236"
     CDS             9010..9459
                     /locus_tag="SI621_003236"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HEI4065909.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQVVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9522..10955
                     /locus_tag="SI621_003237"
     CDS             9522..10955
                     /locus_tag="SI621_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEI4065910.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            11089..12159
                     /gene="ygjJ"
                     /locus_tag="SI621_003238"
     CDS             11089..12159
                     /gene="ygjJ"
                     /locus_tag="SI621_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HEI4065911.1"
                     /translation="MKLITAPCRALLALPFCYAFSAVAEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALQVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GIMASAMYEALKGVYLSTAYTYAKHRHDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            12176..14527
                     /gene="ygjK"
                     /locus_tag="SI621_003239"
     CDS             12176..14527
                     /gene="ygjK"
                     /locus_tag="SI621_003239"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEI4065912.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNAINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     SKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYSATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLPNGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14819..>15671
                     /locus_tag="SI621_003240"
     CDS             14819..>15671
                     /locus_tag="SI621_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065913.1"
                     /translation="MVTPCHIKASAGMTLPLALENQESCRTRLEEMINLINRNTDCST
                     GVPDQVNALVATMADALQPYTVMTNELPEYPNMPERVLYIPEPFSFCFSGHTITFTNT
                     KRDYNGDNLVNIAVSKDGGNARGDADCALFHAFVTKMMSRSRQPGTYDFFPSLKEEKQ
                     GEGMHFMVTPRNSSISAGAIMSRPLLQTQHGCEILNKIINLINADTDEVNELVVAMTN
                     ALFLYQTVVRTELQRTEPFTEPFRFSFFFSSHTITFTKAESNSKLVQIEVSRGGVTVR
                     ECVGSARF"
BASE COUNT         3833 a         4128 c         4040 g         3670 t
ORIGIN      
        1 atttgtagaa ctgatatatc tgaagtatga gaagacattc agcgtcgtag caaggctttc
       61 ggatgcggcg tgaacgcatt atccggcctg gatgtgaggt gcatccacgt ttaaaaatca
      121 tttttatact tgcaagagcg ctcatatcag atataaataa gatatatcta atcaagcaaa
      181 aggagactga tatgagccat catcacgaag ggtgttgtaa acatgaaggc cagccacgcc
      241 atgaaggctg ctgcaaaggt gagaagtcag aacatgagca ctgcggacac ggtcaccagc
      301 atgaacacgg tcaatgctgc ggtggtcgcc acggtcgcgg cggcggtcgt cggcagcgtt
      361 tctttggtca cggtgaattg cgtctggtga ttctggatat tctctcgcgc gatgacagcc
      421 acggttacga gttgattaaa gcgattgaga atctaaccca ggggaattac accccaagcc
      481 cgggcgtcat ctacccgacg ctggattttc tccaggagca gtcgctgatt accatccgcg
      541 aagaggaagg tggtaagaag cagattgcgc tgaccgaaca gggcgcgcag tggctggaag
      601 aaaaccgcga acaggtggag atgatcgaag aacgcatcaa agcgcgttgc gttggcgctg
      661 cactccgcca gaacccgcaa atgaagcggg cactggataa ttttaaagcg gtgctggatt
      721 tacgcgtcaa ccagagcgat atcactgatg cgcagataaa aaagatcatt gcggtgatcg
      781 accgcgccgc ttttgatatt acgcaactgg attaatcacc gattgccgga tgcggcatat
      841 cgattaatgc agcaccgtca ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc
      901 cgacacctgc gcactctctt ccaccagctc ggcatttttc tgggtgatga ggttaagctc
      961 atccactgca cgggtcaggc tggaaagccc atcggcctgt tccagcgttg aatggctaat
     1021 ctgagctatc aactgggtga cgtttttcac ctgtgcaaca atatcttcca tcgtccgtcc
     1081 ggctgcgtgt acctgctgcg aaccggattg caccttatca gcactggcat caatcagctt
     1141 gcgaatatcg ttggcagcat tggcgctgcg gctggctaaa tgacgcactt cccctgccac
     1201 cactgcaaaa cctttgcctt gttcgccggc acgcgccgct tccaccgccg cattcagggc
     1261 cagaatattg gtctgaaacg caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg
     1321 ggtactgtcg gcgatatcgt ccattgtctt gatcaccgtg gtcatcgcct ccccgccctg
     1381 cactgccgca ttactggcag tgatagaaag tttatcggca gccgacgccg tggcagagtt
     1441 ctgtttcacc gacgccgcca tttggttcat ggtcgccacc gtttgctgaa cgttatcaac
     1501 tgtctgctgg gtatgttcgt tcagttcatc ggtacctttc gccagcgtct cactgccgtt
     1561 tctgacactg gacacctggc ttgagacatc gttaattagc caacggcaca tcaggccaag
     1621 ctgccctacc gcgcgtaatg tcagccccag ctcatcgctg cgattcagat gctcaacact
     1681 attacgctct ccggtcgcca ccttcagtgc ctgacgggcg acattttcta tcgggcggac
     1741 aatctgccat tcaaaacagg cgcttgccaa caataccact aacgcacaga ggatatacgt
     1801 caccaccggg gcagcaacaa accaaagcat gaccgccagc aagataaaca tcagggtcat
     1861 cacgccacgc gcccgccagc gaagcggtaa tgaaggcagt ttacccagcc agcctttacg
     1921 caccaccagg cctttatgaa tacgcttact ggtacgtccg gcgttcaacg ctttgtacag
     1981 cggctccacc gccgcgatct cttcatccgt cgcccgggta cgaatcgaca tatagccact
     2041 gatttttccc tcgcgcacca tcggtaccgc attggcccgc acccaataat ggtcgccatt
     2101 tttgcggcga tttttcacga tgccgctcca gggctcccct tttttcaggg tgaaccacat
     2161 atctgcaaac gccgcttttg gcatatccgg gtgacgcacc atgttgtgcg gctgcccttg
     2221 taactcttgc aaggtaaagc cgctcacctg cacaaaagtg tcattagcat gagtgatata
     2281 gctttgcaga tcggtagtgg acatcagagt ggtatcgtcc gccagcgggg tattttgctg
     2341 ggtgacatac ggatgagaag acatgttcgc gtcctgtgca ggttatatgg ttgttaactt
     2401 cttgtcagag tttatgtcgg ccccgctgcg gttatcttta actgattaat ttgatttaga
     2461 tcgcaatttg tgattaaagc ataaatctaa tcccttgatt taaaatactt tcactctgtt
     2521 actatacgaa aacgttaatt atcttgccca aaaatcaggc agttgttgcc ctgaaagtgt
     2581 gcatttgtga agcaatcgcc aaatccatac ccgacaaaaa ccgtgcaaaa taacaacata
     2641 tgttaacaaa tagcattaaa tattgcacaa atgataaccg aatttgtgtt tatcccgatt
     2701 ttcgcgatcg cagccggagt ggcgcaatcc ctgcaatact taaatcggta tcatgtgata
     2761 cgcgagcctc cggagcatat tttgaacagg ttaccttcga gcgcatctgc tttagcgtgc
     2821 agcgcccacg ccctgaatct cattgaaaag cgaacgctgg atcatgagga gatgaaagca
     2881 cttaaccggg aggtgattga atacttcaaa gagcatgtca atccggggtt tttagagtat
     2941 cgcaaatctg ttaccgccgg cggggattac ggagccgtag agtggcaagc gggaggttta
     3001 aatacgcttg tcgacaccca gggacaggag tttatcgact gccttggagg ttttggaatt
     3061 ttcaacgtgg ggcaccgtaa tccagttgtg gtttccgccg tacagaatca acttgcgaaa
     3121 caaccgctgc acagccagga gctgctcgat ccgttacggg cgatgctggc gaaaaccctt
     3181 gcggcgctaa cgcccggtaa actgaaatac agcttcttct gtaatagcgg caccgagtcc
     3241 gttgaagcag cgctgaagct ggcgaaggct tatcagtcac cgcgcggcaa gtttactttt
     3301 attgccacca gcggcgcgtt ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa
     3361 tcgaccttcc gcaaaccatt tatgccgctg ctgccagggt tccgtcatgt accgtttggc
     3421 aatatcgaag ccatgcgcac ggcccttagc gagtgcaaaa aaaccggtga tgatgttgct
     3481 gcagtgatcc ttgagccgat tcaaggtgaa ggtggcgtca ttctgccgcc gccgggctat
     3541 ctcaccgccg tgcgtaagct gtgcgatgag ttcgacgcac tgatgatcct cgatgaagtg
     3601 caaaccggta tggggcgcac gggcaagatg ttcgcctgcg agcatgagaa cgtacagccg
     3661 gacatcctgt gtctggcaaa agccctcggc ggcggcgtga tgccgattgg cgcaaccatc
     3721 gctactgaag aggtgttttc cgtcctgttc gacaacccgt tcctgcatac caccaccttt
     3781 ggcggcaacc cgctggcctg tgcggcggcg ctggcgacca tcaatgtgtt gctggaacaa
     3841 aacttaccgg ctcaggctga acaaaaaggc gatatgttgc tggacggttt ccgtcaactg
     3901 gcgcgggaat atcccgatct ggtacaggaa gcgcgtggta aagggatgtt aatggcgatt
     3961 gagtttgttg ataacgaaat cggctataac tttgccagcg agatgttccg ccagcgcgtt
     4021 ctggtggccg gaacgctcaa taacgccaaa acgatccgca ttgaaccgcc actgacactg
     4081 accattgaac agtgtgaact ggtgatcaaa gcggcgcgta aggcgctggc agccatgcga
     4141 gtaagtgtcg aagaagcgta atatcaaatc ggatggcgat acgacgtcgc catccgatct
     4201 ttttatacga tacgtacgcc cgcaggcatc atccgctccg gcgttaacag cacgctttca
     4261 ctgccgtcat cggtttccgc acacaacagc atgcattccg acgtttcacc gcgcattttc
     4321 gctttttgca gattgcataa gaccaccacc gtttttccca tcagctcttc ttcgctgtag
     4381 tacggcacca ggctggtcac ggtttgcagc gttttttcac ccacatcaac ctgtacgatg
     4441 tacagcttgt cggcgttttc atgacgtttc acttccacaa tctttccgac gcgcatttcc
     4501 agacgtgcaa aatcagcgta agccacggtt tccattgttt cctcccttcg ggtaaaattt
     4561 tactaaacta tagcaaaagt ttttctcaat cctgtaggct aaaaatggag aatgcaggcg
     4621 tgatcacatt cgcaagccgc tgtgttaccg ttacagcgtc aaagaaacgc gctttattta
     4681 ctgaaaacag gtgatccgat aagcacttcc tctacaatgg aggcgcacat cagggaaagt
     4741 aaaaaaggta aacatggcaa cactaaaaga catcgcaatc gaagctggcg tatccctggc
     4801 gacagtatcc agggtcttaa atgacgatcc gacattgaat gtgaaagaag agacgaaaca
     4861 tcgcattctc gagatcgccg aaaagctgga gtacaagacc agtagcgccc gtaaactcca
     4921 gacaggcgca gttaaccaac accatattct ggctatctac agctaccagc aggagctgga
     4981 gatcaacgat ccttactatc tggcgatccg ccacggcatt gaaactcagt gcgaaaagct
     5041 ggcgatcgaa ctgaccaact gttatgaaca caacggctta ccagacatta aaaacgtcac
     5101 cggtatttta attgtcggca aacccacgcc cgccctgcgc gccgccgcct gtgcgttgac
     5161 cgacaatatc tgttttatcg actttcacga acctagcagc ggttacgatg cggtggatat
     5221 cgatctggca cgcatcagta aagaaatcat cgacttctat atcaaccagg gcgttaatcg
     5281 tattggtttt attggcggtg aagatgagcc aggcaaggcg gatattcgtg aggtcgcctt
     5341 tgcggaatat ggccgactga aacaagtggt acgcgaagag gatatctggc gcggcggttt
     5401 ttccagttcg tcgggctatg aactggcaaa acagatgctg gcgcgggaag actatccgaa
     5461 ggcactgttt gttgcttccg attccattgc tatcggcgta ctgcgggcga ttcatgaacg
     5521 tggcctgaac atcccacagg atatttcact tatcagcgtt aatgatatcc ccaccgcgcg
     5581 atttaccttt ccgccgctct ccaccgtgcg catccattcc gaaatgatgg gaagccaggg
     5641 cgttaacctg gtgtatgaaa aagcccgcga tggtcgcgcg ctgccgctgt tagtcttcgt
     5701 tcccagcaaa ttaaaactgc gcggcacgac ccgttaaatc cccttacaca ctgtccggca
     5761 atcgtttttg ccggacagtg ctgccgttta ttttcgtgat ccagttaaag taaatgcatt
     5821 tacccgctac tttttagtaa aaattttact aaactcccca gcaattacac aaactaccat
     5881 caccataaat ggttccgatt tctctctacc gggaggccct atgaatcgct gggaaaacat
     5941 tcagctcacc cacgaaaacc gacttgcgcc gcgtgcgtac tttttttcat atgattctgt
     6001 tgcgcaagcg cgtacctttg cccgcgaaac cagcagcctg tttctgccct taagcggtca
     6061 gtggaatttc cacttttttg accacccgct gcaagtgcca gaagccttca cctctgagtt
     6121 aatggctgac tgggggcata ttaccgtccc cgccatgtgg caaatggaag gtcacggcaa
     6181 actgcaatat accgacgaag gttttccatt ccccatcgat gtaccgtttg ttcccagcga
     6241 taacccaacc ggtgcctatc aacgtatttt caccctcagc gacggctggc agggtaaaca
     6301 gacgctgatt aaatttgacg gcgtcgaaac ctattttgaa gtctacgtta acggtcagta
     6361 tgtgggtttc agcaagggca gtcgcctgac cgcagagttt gacatcagca cgatggttaa
     6421 aaccggcgac aacctgttgt gtgtgcgcgt gatgcagtgg gcggattcca cctacgtgga
     6481 agaccaggat atgtggtggt cggcggggat ctttcgcgat gtttatctga tcggaaaaca
     6541 actaacacat attaacgatt tcaccgttcg taccgacttt gacgaagcct attgcgatgc
     6601 cacgctttcc tgcgaagtgg tgctggaaaa tctcgccgcc tcccctgtcg taacgacgct
     6661 ggaatatacc ctgtttgatg gggaacgcct agtgcacagc agcgccattg atcatttggc
     6721 aattgaaaaa ctgaccagcg ccagctttgc ttttactgtc gaacagccgc agcaatggtc
     6781 agcggaatcc ccttatcttt accatctggt catgacgctg aaagacgccg acggcaacgt
     6841 tctggaagtg gtaccacaac gcgttggctt ccgtgatatc aaagtgcgcg acggtctgtt
     6901 ctggatcaat aaccgttatg tgatgctgca cggcgtcaac cgtcacgaca acgatcatcg
     6961 caaaggccgc gccgttggaa tggatcgcgt cgagaaagat ctccagttga tgaagcagca
     7021 caacatcaac tccgtgcgta ccgctcacta cccgaacgat ccgcgttttt acgaactgtg
     7081 tgatatctac ggcctgtttg tgatggcgga aaccgacgtc gaatcgcacg gctttgctaa
     7141 tgtcggcgat atcagccgta ttaccgacga tccgcagtgg gaaaaagtct acgtcgagcg
     7201 cattgttcgc catattcacg cgcagaaaaa ccatccgtcg atcatcatct ggtcgctggg
     7261 caatgaatcc ggctatggct gtaacatccg cgcgatgtac cacgcggcga aggcgctgga
     7321 tgacacgcga ctggtgcatt acgaagaaga tcgcgatgct gaagtggtcg atattatttc
     7381 caccatgtac acccgcgtgc cgctgatgaa tgagtttggt gaatacccgc atccgaagcc
     7441 gcgcatcatc tgtgaatatg ctcatgcgat ggggaacgga ccaggtgggc tgacggagta
     7501 ccagaacgtc ttctataagc acgattgcat tcagggacat tatgtctggg aatggtgcga
     7561 ccacgggatc caggcgcagg atgacaacgg caatgtctgg tataaattcg gcggcgacta
     7621 cggcgactat cccaacaact ataacttctg tcttgatggt ttgatctatt ccgatcagac
     7681 gccgggaccg ggcctgaaag agtacaaaca ggttattgcg ccggtaaaaa tccacgcgct
     7741 ggatctgact cgcggcgaac tgaaagtcga aaataaactg tggtttacca cgcttgatga
     7801 ctacaccctg cacgcagagg tgcgcgccga aggtgaaacg ctcgcgacgc agcagattaa
     7861 actgtgcgac gttgcaccga acagcgaagc ccccttgcag atcacgctgc cgcaactgga
     7921 cgctcgtgaa gcgtttctca acattacggt gaccaaagat tcccgcaccc gctacagcga
     7981 agccgggcat tctatcgcca cttatcagtt cccgctgaag gaaaataccg cgcagcctgt
     8041 acctttcgca ccgaataatg cgcgtccgct gacgctggaa gacgatcgtt tgagctgcac
     8101 cgttcgcggc tacaacttcg cgatcacctt ctcaaaaatg agtggcaaac cgacatcctg
     8161 gcaggtgaat ggcgagtcgc tgctgacccg cgagccaaag atcaacttct tcaagccaat
     8221 gatcgacaac cacaagcagg agtacgaagg gctgtggcag ccgaatcatt tgcagatcat
     8281 gcaggaacat ctgcgcgact ttgccgtaga acagagcgat ggtgaagtgt tgatcatcag
     8341 ccgcacggtt attgccccac cggtgtttga cttcgggatg cgctgcacct acatctggcg
     8401 catcgctgcc gatggccagg ttaacgtggc gctttccggc gagcgttacg gcgactatcc
     8461 gcacatcatt ccgtgcatcg gtttcaccat ggggattaac ggcgaatacg accaggtggc
     8521 gtattacggt cgtggaccgg gcgaaaacta cgccgacagc cagcaggcta acatcatcga
     8581 tatctggcgc agcaccgtcg atgccatgtt cgagaactat cccttcccac agaacaacgg
     8641 caaccgtcag catgtccgct ggacggcact gactaaccgc cacggcaatg gtctgctggt
     8701 tattccgcag cgcccaatta acttcagtgc ctggcactac acccaggaaa acatccacgc
     8761 tgcccagcac tgtaacgagt tgcagcgcag tgatgacatc accctgaatc tcgaccacca
     8821 gctgcttggc ctcggctcca actcctgggg cagcgaggtg ctggactcct ggcgcgtctg
     8881 gttccgtgac ttcagctacg gctttacgtt gctgccgatt tctggcggag aagctaccgc
     8941 gcaaagcctg gcgtcgtatg agttcggcgc agggttcttt tccacgaatt tgcacagcga
     9001 gaataagcaa tgaggatcat cgataactta gaacagttcc gccagattta cgcctctggc
     9061 aagaagtggc aacgctgcgt tgaagcgatt gaaaatatcg acaacattca gcctggcgtc
     9121 gcccactcca tcggtgattc attgacctac cgtgtggaaa atgactccgc gaccgatgcg
     9181 ttatttaccg ggcatcgacg ctattttgaa gtgcattact acctgcaagg gcagcaaaaa
     9241 attgaatatg cgccgaaaga gacattacag gtagtggaat attatcgtga tgaaactgac
     9301 cgtgaatatt taaaaggctg cggagaaacc gttgaggtcc acgaagggca agtcgttatt
     9361 tgcgatatcc atgaagcgta tcggtttatc tgcaataacg cggtcaaaaa agtggttctt
     9421 aaagtcacca tcgaagatgg ttatttccat aacaaataac aactacggcg gcaaaaggag
     9481 tttgccgcca ccgctaccct acacattttc ggagatgtgt tatgtctgat accaaacgta
     9541 atacaatcgg caaattcggc ttgctctcgc tgacttttgc cgccgtttac agctttaaca
     9601 acgttatcaa taataatatt gagcttggac tggcctcggc accgatgttt ttcctcgcga
     9661 cgatttttta ttttattccc ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa
     9721 actcagaagc tggtgtctac gcgtgggtaa aaagttcgct gggcgggcgt tgggcattta
     9781 ttactgccta tacctactgg ttcgtaaacc tgttcttttt cacctcgctg ttaccgcgcg
     9841 ttattgctta tgcttcgtat gccttcctcg gctacgaata tattatgaca ccggttgcca
     9901 ccaccattat cagtatggtg ctgttcgcct tctccacctg ggtttctacc aacggggcga
     9961 aaatgttggg gccaattacc tccgtcactt caacgctgat gctgctgtta acgctctcct
    10021 acattttact ggcaggcacg gcgctggttg gcggcgtaca gcctgccgat ccgatcactg
    10081 ttgacgcgat gatcccgaac ttcaactggg cgttcctcgg cgttaccacc tggatcttta
    10141 tggccgcagg tggcgcggag tccgtcgcgg tgtacgttaa cgacgtcaaa ggcggttcga
    10201 aatcgttcgt taaagtgatc atcctcgccg ggatttttat cggtgtgctg tattccgtct
    10261 cctcggtact gattaacgtc ttcgtcagca gcaaagagtt gaaatttact ggcggatcgg
    10321 tgcaggtatt ccacggcatg gcggcgtatt ttggtctacc ggaagcgttg atgaatcgct
    10381 ttgtcggtct ggtgtccttt accgcgatgt tcggttccct gctgatgtgg accgcaacgc
    10441 cggtgaaaat tttcttctcc gaaatcccgg aaggcatctt tggtaagaaa accgtcgaac
    10501 tgaacgaaaa cggcgttccg gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc
    10561 tgatgattat cccgatgctc ggttccaata ccgtgcagga tctgatgaat actattatta
    10621 atatgactgc cgcagcgtcc atgcttccgc cgttattcat catgctggct tacctgaatt
    10681 tacgcgccaa attagatcac ctgccacgtg atttccgtat gggttcccgc cgcaccggta
    10741 ttatcgttgt ttcaatgctg attgcgatat ttgccgtagg gtttgtcgct tcgacattcc
    10801 cgactggcgc gaatattctg accatcattt tttataacgt cggcggtatt gttatcttcc
    10861 tcggctttgc gtggtggaaa tacagtaaat atataaaggg attaacggct gaagagcgcc
    10921 atattgaagc gacgccagcc agcaatgttg attaagcaga ctaataaaaa atgagcaggc
    10981 attttccctc ttcctggatg gggagaggga aaatagtttc tgcctttata tctttataat
    11041 tacaacgata aaaggctgta ctttttcttt agctcatgga ttaacacaat gaaattaatc
    11101 actgcaccat gcagagcatt acttgctctg ccgttttgct acgcgttttc tgcggtcgct
    11161 gaagaagctc gtccggcaga acatgacgac acaaaaacac ccgccattac ctcgacatct
    11221 tctccttcat ttcgttttta cggcgaatta ggggttggtg gatatatgga tttagagggt
    11281 gagaataaac ataaatacag cgacggcacc tatattgaag gtggactgga gatgaagtac
    11341 ggctcctggt tcggcctgat ttacggcgaa ggctggaccg tacaggccga ccacgacggt
    11401 aatgcctggg tgcctgacca tagctggggt ggtttcgaag gtgggattaa ccgtttctat
    11461 ggcggttatc gaaccaatga tggcaccgaa atcatgctca gtctgcgtca ggattcctcg
    11521 ctggatgacc tgcaatggtg gggcgacttc acccccgatc tgggctacgt cattcccaat
    11581 acccgcgaca ttatgaccgc gctgcaggtg cagaacttaa gcggcaactt tcgttatagc
    11641 gtcaccgcca cgcctgccgg acatcatgac gaaagcaaag cctggctaca ttttggcaag
    11701 tacgatcgct acgacgacaa atacacctat ccggcaatga tgaacggtta catccagtat
    11761 gaccttgccg aaggcatcac ctggatgaac ggtctggaaa tcaccgacgg cacagggcaa
    11821 ctctatctga cgggactgct tacccctaac tttgccgctc gcgcctggca ccataccgga
    11881 cgcgccgacg ggctggacgt accgggaagt gaaagcggga taatggcgag cgccatgtat
    11941 gaagcgttaa aaggcgttta tctctccacc gcttacacct acgccaaaca tcgccatgac
    12001 cacgctgacg atgaaaccac ctctttcatg cagtttggta tctggtacga atacggcggc
    12061 ggacgtttcg caacggcttt tgatagccgc ttctacatga aaaatgcctc tcacgatccc
    12121 agcgaccaac tcttcctgat gcaatatttc tactggtaat aaggactgta atttcatgaa
    12181 aatcaaaaca attttaacgc cagtaacctg cgctctgctg ataagttttt ccgcccatgc
    12241 cgctaacgcc gacaattata aaaacgcgat taaccgtact ggcgcgccgc agtacatgaa
    12301 ggattacgat tacgacgatc accagcgttt taatccgttt ttcgatctcg gagcctggca
    12361 tggtcatctg ttgccagacg gcccgaacac catgggcggc tttccgggcg ttgcgctgct
    12421 gacggaagag tacatcaact ttatggccag caatttcgac cgcctgaccg tctggcagga
    12481 cagtaagaaa gtcgacttca cgctggaggc atacagtatt cccggcgcgc tggtgcaaaa
    12541 actgacagca aaagatgtgc aggttgaaat gactctgcgc ttcgccacgc cgcgcacgtc
    12601 actactggaa actaaaatca tcagcaataa accgctggat ctggtgtggg acggcgagct
    12661 gctggaaaaa ctggaagcga aagaagggaa accgctttcc gataaaacca ttgctggcga
    12721 gtaccctgac tatcagcgca aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg
    12781 caaagtgcgc gccacctggg atctgctgac ctccggtgaa tcagaatatc aggtgcataa
    12841 atccctgcca gtgcagactg aaatcaacgg taatcgcttt accagtaagg cgcatatcaa
    12901 cggttcgacg acgctctaca ccacctattc ccatctgctg accgctcagg aagttagcaa
    12961 agagcaaatg cagatccgcg atattctggc gcgtccggcg ttttatctca ccgcctcgca
    13021 gcaacgctgg gaagagtatc tgaagaaagg gttaaccaat ccggatgcga cgccggaaca
    13081 gacgcgcgtc gcggtgaaag cgatcgaaac gctcaacggt aactggcgct caccgggcgg
    13141 tgcggtgaaa tataacaccg tcacgccgtc ggtgaccggg cgctggttct ccggcaatca
    13201 gacctggccg tgggatacct ggaagcaggc gtttgcgatg gcgcatttca atccggacat
    13261 cgccaaagag aatatccgcg cggtcttctc ctggcagatc cagcccggcg atagcgtgcg
    13321 tccgcaggat gtgggctttg tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg
    13381 cggcgatggc ggcaactgga acgaacgtaa taccaaaccc agccttgccg cctggtcggt
    13441 gatggaagtg tacaacgtca cccaggataa agcctggttg gcagagatgt acccgaagct
    13501 ggtggcctat cacgactggt ggttacgtaa ccgcgatcac aacggcaacg gcgtgccgga
    13561 atatagcgcg acccgcgaca aagcccacaa caccgagagc ggcgagatgc tgtttacggt
    13621 gaaaaaaggc gacaaagaag agacgcagtc tgggctgaac aactacgccc gtgtggtgga
    13681 gaaaggccag tacgacagtc tggaaattcc ggcacaggtt gctgcgtcgt gggaatcagg
    13741 tcgtgatgac gccgccgtct ttgggtttat cgacaaagaa cagctggata aatatgtcgc
    13801 taatggcggc aaacgtagcg actggacggt gaaattcgcc gaaaaccgca gtcaggacgg
    13861 aacgttgctg gggtactcgc tattgcagga gtcggtggat caggccagct atatgtacag
    13921 cgataaccat tatctggcgg agatggcaac catactcggt aagccggaag aggccaaacg
    13981 ctatcgccag ttggcacagc agctcgcgga ctacatcaac acctgtatgt tcgacccgac
    14041 tacccagttc tactatgacg tgcgcattga agataaaccg ctgccgaacg gctgcgcggg
    14101 caaaccgatt gtcgagcgcg gtaaagggcc agaaggctgg tcaccgctgt ttaacggtgc
    14161 agcaacgcag gccaacgccg acgcggtggt gaaggtgatg ctcgatccta aagagttcaa
    14221 cacctttgtt ccgctgggaa cggcggcgtt aaccaatccg gcctttggcg ctgatatcta
    14281 ctggcgcggg cgcgtatggg tggatcagtt ctggtttggt ctgaaaggaa tggagcgtta
    14341 cggttatcgc gatgatgccc tgaagctggc ggatacgttc ttccggcacg ccaaagggtt
    14401 aaccgccgat ggaccaattc aggaaaatta caacccgctg acaggcgcac agcaaggcgc
    14461 acccaatttc tcctggagcg ccgcgcattt gtatatgttg tataacgatt ttttccgtaa
    14521 gcagtaattt gcaacagctg gcggatgcgg cgtgaacgcc ttatccgccc tggcccgcgg
    14581 catcgggggt atacgcactc ttttctttta aactgcggta aaaaccggaa tgcaactgac
    14641 cgaaattaat aaaataatac tgatttgccg tctgacgttt aatggtccga cggttatgcg
    14701 ccctgttcac tgactggtgc tggagctgtt gagttgaaca aacgggtcag ccgctctccg
    14761 gggggccagt gagcaaacac ggcactatct gcccccctga gagaaaggaa ttcattacat
    14821 ggtaacacca tgccacatca aagccagtgc aggtatgaca ttgcccctgg ccctggagaa
    14881 ccaggagagc tgcaggacac gtctggaaga gatgataaat ttaatcaaca ggaatacaga
    14941 ctgcagcacg ggtgttcctg accaggttaa cgcactggtg gctaccatgg cggacgctct
    15001 gcagccgtac acagtgatga cgaacgaact gccggagtat ccgaacatgc cggaacgtgt
    15061 cctgtacatt cctgagccgt tcagtttctg tttttccggc cacaccatca ccttcacgaa
    15121 cacgaaaagg gattacaacg gggacaatct cgtgaacatt gcggtcagca aggacggggg
    15181 gaatgcccgt ggcgatgctg actgtgccct gtttcacgcc tttgtcacaa aaatgatgtc
    15241 acgcagtcgc cagccaggaa catatgattt ctttccctca ctcaaggaag aaaaacaagg
    15301 ggaaggaatg catttcatgg taacaccacg caactccagc atcagcgcgg gcgcgataat
    15361 gtctcggccc ctgctgcaga cccagcacgg ctgtgaaatt ctgaataaga taataaattt
    15421 aatcaacgcg gatacggacg aggttaatga actggtagtt gccatgacga acgcgctgtt
    15481 tctgtatcag acggtggtga ggactgagct gcaacgcact gagccattca ctgagccgtt
    15541 caggttcagt ttcttttttt ccagccacac catcaccttt acgaaggcgg agagtaatag
    15601 caaattagtg cagattgagg tcagtagggg cggggtgacg gtccgtgaat gtgttggcag
    15661 tgcccggttt c
//
LOCUS       DARNVM010000113        15517 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.113, whole genome shotgun sequence.
ACCESSION   DARNVM010000113 DARNVM010000000
VERSION     DARNVM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.113"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..798)
                     /locus_tag="SI621_003241"
     CDS             complement(<1..798)
                     /locus_tag="SI621_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4065914.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKHDLNQNLIERLWNKIRDFF
                     LGSDKQKAFKSIHKYINTLSVLNYNSASSPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDLGNCNCSNVNFEHTYFDSVKFTNT
                     NCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQ"
     gene            complement(1029..2003)
                     /gene="yajO"
                     /locus_tag="SI621_003242"
     CDS             complement(1029..2003)
                     /gene="yajO"
                     /locus_tag="SI621_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HEI4065915.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEGIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2058..3920)
                     /gene="dxs"
                     /locus_tag="SI621_003243"
     CDS             complement(2058..3920)
                     /gene="dxs"
                     /locus_tag="SI621_003243"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEI4065916.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3945..4844)
                     /gene="ispA"
                     /locus_tag="SI621_003244"
     CDS             complement(3945..4844)
                     /gene="ispA"
                     /locus_tag="SI621_003244"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HEI4065917.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(4844..5086)
                     /gene="xseB"
                     /locus_tag="SI621_003245"
     CDS             complement(4844..5086)
                     /gene="xseB"
                     /locus_tag="SI621_003245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEI4065918.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5292..6740
                     /gene="thiI"
                     /locus_tag="SI621_003246"
     CDS             5292..6740
                     /gene="thiI"
                     /locus_tag="SI621_003246"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEI4065919.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(6794..7384)
                     /gene="yajL"
                     /locus_tag="SI621_003247"
     CDS             complement(6794..7384)
                     /gene="yajL"
                     /locus_tag="SI621_003247"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HEI4065920.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETIKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7347..8258)
                     /gene="panE"
                     /locus_tag="SI621_003248"
     CDS             complement(7347..8258)
                     /gene="panE"
                     /locus_tag="SI621_003248"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEI4065921.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8426..8917
                     /gene="yajQ"
                     /locus_tag="SI621_003249"
     CDS             8426..8917
                     /gene="yajQ"
                     /locus_tag="SI621_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HEI4065922.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9045..10409)
                     /locus_tag="SI621_003250"
     CDS             complement(9045..10409)
                     /locus_tag="SI621_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4065923.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(10558..11448)
                     /gene="cyoE"
                     /locus_tag="SI621_003251"
     CDS             complement(10558..11448)
                     /gene="cyoE"
                     /locus_tag="SI621_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEI4065924.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGRIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11460..11789)
                     /locus_tag="SI621_003252"
     CDS             complement(11460..11789)
                     /locus_tag="SI621_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEI4065925.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11789..12403)
                     /locus_tag="SI621_003253"
     CDS             complement(11789..12403)
                     /locus_tag="SI621_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEI4065926.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12393..14384)
                     /gene="cyoB"
                     /locus_tag="SI621_003254"
     CDS             complement(12393..14384)
                     /gene="cyoB"
                     /locus_tag="SI621_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEI4065927.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14406..15353)
                     /gene="cyoA"
                     /locus_tag="SI621_003255"
     CDS             complement(14406..15353)
                     /gene="cyoA"
                     /locus_tag="SI621_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HEI4065928.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMNMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3880 a         4035 c         3926 g         3676 t
ORIGIN      
        1 ttgatctgct tctggatatt gcatctcagg ttctttttct ttaccatata tcaccgcatt
       61 atcaagaatt gcgccagtgc aattcatatt tgttagatcg catttgataa atctacaact
      121 gttaaaattc gcatttgtac aattcgtatt tgtaaacttg acgctatcaa aataagtatg
      181 ttcgaagttg acatttgagc agttacagtt tcccagatct ataccgaaat ctatatcggg
      241 atctttattg ggatctttac cggaaatatt gctgtgtgct agatatagac cgctgaaatc
      301 gtaatatcca ttgtcatcct tttctattgg gagtgaataa gtctttacta cctcgttaac
      361 aatagagata gttccacgta tcatcagatt ttctccgact ttataaaaaa taatgttatg
      421 tttgatggtg ctgttgtatg gctctggcaa tgaggatgca attttattcc agaaacaaca
      481 aagcgtggtc tgcttttgtt tgggcgagag gttatcaaat ttcaggttta aatatgagtc
      541 taaatctgac gatgcatcta tattaaaatt gggatctgga gatgacgctg aattataatt
      601 tagaactgac aatgtattga tgtatttatg aatcgattta aatgcttttt gtttatcact
      661 gcccagaaaa aaatcacgaa ttttgttcca tagcctttcg ataagatttt gatttaaatc
      721 gtgcttcatt gagtttctta aatctgcaac cggtacatca gtaaacagga tcggttgttt
      781 ggtgatagtt ttcatcatga ttgtaatcct cttacgttga ctaaataaga cagttttagg
      841 gagtattacc tggagaaact atcaaaaatc gctcaaaggg atgaagatga tgaatagctg
      901 caaactcatt cacgaagaat ttctcttgca gaggtggatt atggttacag aagatgatgg
      961 gaggtacgtt gtccccatca tcgcagacga tggggaattc agcagataaa atggctgaaa
     1021 agcgaaattt acttaaatcc tactaccgga tgcggtttat acggcgtttc cagttcagca
     1081 atctgttccg gcttcaaggt gatatccacc gcgttcagca gctcatcaag ctgttcttcc
     1141 cgcgaagttc cgataatcgg tgcggcaatg cccggtttac tcaacaacca ggccagcgca
     1201 acttgtgctc gtgttgcacc cagttcttca ctgacgcccg ttaaccgctc tgcgatctgc
     1261 gcgtcatttt catcgctttc tttatagaga tttttcccca cctcatcaga caccagtcgt
     1321 gcggtagttt ctccccacgg acgtgtcaga cgaccccgcg ccagcgggct ccatggaatc
     1381 accgctacgc cctcctggta acacagtggc agcatctcgc gctcttcttc tcgataaatc
     1441 agattgtagt gatcctgcat actgacaaac tgcgcccagc cgtgctgttt ttgtagttcc
     1501 agtgcctgag caaactgcga agcgtgcatg gatgacgcgc cgatataacg cgctttcccg
     1561 gcttttacca cgtcgttgag ggcttccagc gtctcttcga tcggcgtgtt gtaatcccag
     1621 cgatgaattt gcaggatatc gacataatcc atgccgagac ggcgcaggct gtcgtcgata
     1681 gagcgcaata tttgcgcacg ggataatcct tccggcaaat caccaacgcg atggaacact
     1741 ttggtggcaa cgaccacgtc ttcacgacgg gcgaaatccc gcagtgcgcg accaacgatc
     1801 ccttcgctgc tgccatcgga atagctattg gcggtatcaa agaaatttat gccgccttcc
     1861 agcgcgcgtt taatgatggg acggctgctt tcttccggca gtgtccatgc gtgattacca
     1921 cgatctggct cgccaaaggt catacagccg aggcaaagtc gggaaacgcg aaggtcggtt
     1981 tttcctaagg ggttgtattg catgctgcca ctcctgccat gaataatttc ttaagcatag
     2041 caggagtaga gtagggatta tgccagccag gccttgattt tggcttccat accggcggca
     2101 tcgaggccga gttcggcgcg catttcttcc tgtgttcctt gtggaataaa gaagtcaggc
     2161 aggccaatgt tcagcacggg tactggttta cgatgggcca tcagcacttc gttcactccg
     2221 ctgcctgcgc cgcccataat ggcgttttct tctacggtga ccagcgcttc atggctggcg
     2281 gccatttcca gaattaacgc ttcatcaagc ggtttcacaa aacgcatatc gaccagcgta
     2341 gcgttcagcg attcagcgac tttcgccgct tctggcatca gcgtaccaaa gttaaggatc
     2401 gccagtttct cgccacgacg cttcacaatg cctttgccaa ttggcagttt ttccagcggc
     2461 gtcagttcca cgccaaccgc gttgccacgc gggtagcgta ccgctgacgg gccatcgtta
     2521 tagtgatagc cggtatagag catctggcga cattcgtttt catcgctcgg ggtcataatg
     2581 accatttccg gaatacagcg caggtaagag agatcaaaag cgccctggtg ggtttgacca
     2641 tcagcaccaa caatgcccgc gcggtcgatg gcgaacagga ccgggagttt ttgaatcgcc
     2701 acgtcatgca gcacctgatc ataggcgcgt tgcaggaagg tggagtagat cgcgacaatg
     2761 ggtttgtacc caccaatcgc cagacccgca gcaaaggtca ctgcgtgttg ctcggcaatt
     2821 gccacatcga agtagcgatc cgggaattta cgtgaaaact cgaccatgcc ggaaccttca
     2881 cgcatcgccg gagtaatcgc catcagcttg ttatctttcg ctgccgtttc gcacaaccag
     2941 tcgccaaaga tttttgaata gcttggtaaa ccgccgctac ttttcggcaa acaaccgctg
     3001 gagggatcaa atttaggcac ggcgtggaag gtgattgggt ctttttctgc cggttcataa
     3061 ccacgacctt ttttggtcat gatatgcagg aactgcgggc ctttcaggtc gcgcatgttc
     3121 ttaagcgtgg tgataagccc cagcacatcg tgaccgtcaa ccgggccgat gtagttaaag
     3181 cccagctctt caaacaacgt gccaggcacc accatgcctt taatatgttc ttcggtacgt
     3241 ttgagcagct ctttaatggg gggcacgcca gagaaaactt ttttcccgcc ttcgcgcagt
     3301 gaagagtaaa gcttaccgga aagcagctgc gccagatggt tgttgagcgc gccaacattt
     3361 tcggaaatcg acatttcatt gtcgttgaga accaccagca tatcaggacg gatatcgccc
     3421 gcgtgattca tcgcttcaaa cgccatgcca gcggtaatcg caccgtcgcc aatcacacaa
     3481 acggtgcggc gatttttgcc ttctttttcg gcagcgaccg caataccaat tccggcactg
     3541 atggaggttg atgaatgccc gacgcttaat acgtcatatt cgctttcgcc gcgccacggg
     3601 aacgggtgca gaccgccttt ctgacggatg gtgccgatct tgtcgcggcg tccggtcaaa
     3661 attttatgcg gataagcctg atgccccaca tcccaaatca attggtcaaa cggggtgttg
     3721 tagacatagt gcagcgccac ggtcagttcg accgtgccca gcccggaggc gaagtgcccg
     3781 ctggaacggc tcacgctgtc gagtaaatag cggcgcagtt cgtcgcagag tttcggtaaa
     3841 ctctctttcg gcaacagtcg taactcctgg gtggagtcga ccagtgccag ggtcgggtat
     3901 ttggcaatat caaaactcat caggggccta ttaatactta ttgtttattt attacgctgg
     3961 atgatgtagt ccgctagcgc ttccagtgcc gaggtatcga gtgactgctc agccagttgt
     4021 ttcagcgact gacgggcatc gtcgatcaga tcccgggctt tcttccgggc ttgctcaaga
     4081 cccagaagtg cagggtaggt acttttacca agttgctggt cggcaccctg gcgttttccc
     4141 aacgttgcag tatctcctac cacatccagg atgtcatcct gaacctggaa ggcaaggccg
     4201 atgctctctg cgtacttgtc gagtactggc agagcacgac gtcctttatc tccggcgctt
     4261 aatgcaccaa ggcgaacggc ggcgcgaatc aatgcgccgg ttttatgacg atgaatacgc
     4321 tcaagcgcgt ccagaggtac gtgtttgcct tccgcgtcta aatctaatgc ctgaccaccg
     4381 cacattccgg caataccgct ggcgctcgcc agttctgaaa tcatcgaaat tctgtcgcga
     4441 tccgacactt ccggcatatc ggcatcgctt aaaatcgaga acgccagcgt ttgtaaagcg
     4501 tcaccagcga gaatcgcgtt tgcttcgcca aacttcacat ggcaggttgg caaaccgcga
     4561 cgcagatcgt catcatccat tgccggtaaa tcatcatgaa ttaatgagta agcgtggata
     4621 cactctacgg cggcagcggg cgcgtccagc gtgtttgtgc taacgccgaa catatgaccg
     4681 gtggcataaa ccaggaaagg tcgcaggcgc ttaccaccta ataatgcgcc atactgcatg
     4741 gtttcgacca cgggagtgtt ctgaaagggc agtggggcga taaaacggct cagcgcctgg
     4801 ttggcctgct taacgcaggc ttcgagttgc tgcggaaagt ccattactca ttgtccggtg
     4861 taaaaggggt tagagaggcg tcttcattgt cagacagcag aatttgtacg cgctgttcgg
     4921 cttgttgtaa tttagcctgc ccctgacgtg ccagctgcac gccgcgttcg aactcgttca
     4981 gcgcctcttc cagcggcagg tcaccacttt ccagacgggt tacaatctgt tccagctcgc
     5041 tcagcgcctt ttcaaagctg gcgggcgcct catttttctt cggcataatg aatgtctgac
     5101 tctcaatatt tttcgccccg tcatggtaac ggactcaggg caaatagcaa ataacgcgca
     5161 atggtaaggt gatgtgcgca gcaaagcgat gttagtggta tacttccgcg cctggatgca
     5221 gccgcaggtg tgggctgctg tatttttccc tatacaagtc gcttaaggct tgccaacgaa
     5281 ccattgccgc catgaagttt atcattaaat tgttcccgga aatcaccatc aaaagccaat
     5341 ctgtgcgctt gcgctttata aaaatcctta ccgggaacat tcgtaacgtt ttaaagcact
     5401 atgatgagac gctcgctgtc gtccgccact gggataacat cgaggttcgc gcaaaagatg
     5461 aaaaccagcg tctggctatt cgcgacgccc tgacccgtat tccgggtatc caccatattc
     5521 tcgaagtcga agacgtgccg tttaccgaca tgcacgatat tttcgagaaa gcgttggttc
     5581 agtatcgcga tcagctggaa gggaaaacct tctgcgtacg cgtgaagcgc cgtggcaaac
     5641 atgattttag ctcgattgat gtggaacgtt acgtcggcgg cggtttaaat cagcatattg
     5701 aatccgcgcg cgtgaagctg accaatccgg atgtgactgt ccatctggaa gtggaagacg
     5761 atcgtctcct gctgattaaa ggtcgctacg aaggtattgg cggtttcccg atcggcaccc
     5821 aggaagatgt gctgtcgctc atttccggtg gtttcgactc cggtgtttcc agttatatgt
     5881 tgatgcgtcg cggctgccgc gtgcattact gcttctttaa cctcggcggc gcggcgcatg
     5941 aaattggcgt gcgtcaggtg gcacattatc tgtggaaccg ctttggcagc tcccaccgcg
     6001 tgcgttttgt cgctattaat ttcgagccgg tcgtcgggga aattctcgag aaaatcgacg
     6061 acggtcagat gggcgttatc ctcaaacgta tgatggtgcg tgccgcgtct aaagtggctg
     6121 aacgttacgg cgtacaggcg ctggtcaccg gcgaagcact gggccaggtg tccagccaga
     6181 cgctgactaa cctgcgcctg attgataacg tctccgacac gctgatcctg cgtccgctga
     6241 tttcttacga caaagagcac atcatcaacc tggctcgcca gattggcact gaagactttg
     6301 cccgcacgat gcccgaatat tgcggtgtga tctccaaaag cccgacggtg aaagcggtta
     6361 aatcgaagat tgaagcggaa gaagagaagt tcgacttcag cattctcgat aaagtggttg
     6421 aggaagcgaa taacgttgat atccgcgaaa tcgcccagca gaccgagcag gaagtggtgg
     6481 aagtggaaac cgtcaatggc ttcggcccga acgacgtgat cctcgatatc cgttctatcg
     6541 atgaacagga agataagccg ctgaaagtcg aagggattga tgtggtttct ctgccgttct
     6601 ataaactgag caccaaattt ggcgatctcg accagaacaa aacctggctg ctgtggtgtg
     6661 agcgcggggt gatgagccgc ctgcaggcgc tctatctgcg cgagcagggc tttaacaatg
     6721 tgaaggtata tcgcccgtaa tttgtgtttt ttacgtcgca tctggtcaga tgcgacgttt
     6781 gccgcatccg acactactcg taataattat aaatccctgc cgccatcacc agttgtgatg
     6841 ccacttcatg ggctttttca cgcccaacca gcaggtcgat aattttcaga ccaaagtcga
     6901 tagctgtacc cggcccctga ctggtcagca attttacccg tgcatcccag acgacgcgct
     6961 tgtcctgcca ttgttcggcg ggtattttgt ctttcagcgt cgggaagccg gtcatattgc
     7021 caatcgggaa gatatcgtgc gggaccagca cggtggctgg tgcggcgcaa atagccgcga
     7081 cgatacgccc ggaacggtgg aactgtttaa tggtttcaac cagcagggtg ctatcgcgaa
     7141 aacactctgc gcctttaatg cctccaggca gcacgatcac gtcatattcg ccatcagcta
     7201 cttcgaccag cggcgcatcc gccagcagct tcacgccgcg cgagcaggta atcgccaggt
     7261 taccatcgct ggcgacgctg gcagtggtga ctttgatacc gccgcgaacc agcagatcga
     7321 tagtggtgac ggcttcagtc tcttcactac caggggcgag gcaaaccagt gccgatgcgc
     7381 tcatattcac tctcctttct ttttaccatt tcaaacaggc gggtgttttc cggtacggca
     7441 atcccatgcg cgcgggcgcg gcgtaagaga aaaccattga tatagtcgat ttcggtgtgg
     7501 cgcagcgcgc ggatatcctg caacatcgac gagatatttt ccgctgtggc atcaatcacc
     7561 tgcatcacgt aatcacgcaa atcttctgct gaagtatgat gcccttcgcg ttcgattact
     7621 gccgcgactt cttcgcatat ctgcataatt tcttgcggat gatgacgtaa ttcaccattc
     7681 gggcaattcc agatggcggt cagtggatta atcacacagt tgactgccag cttgcgccac
     7741 agctcggcgc gaatattgtt atgccacgcg acgtcaggta acacggtttg caaaatatcc
     7801 gccagataac tgtaatcgcc gtcctgttgc cgtgccggac caatatgcgt gattccgttt
     7861 gccacatgaa taatgacatt gccgtcgcgg cgggcggcat gggtggtggt gcccatcagt
     7921 aatggctgct gaatgttttg caactcttcg atggtgccca tgccgttgtg aattaacagt
     7981 attggcgtag ttacaggcag tgtggacgcg aggcttttga cggcatcgga aacctgccat
     8041 gctttcagcg tcaccaggag cagatcgctg gtggcgagaa aatcgggatc gttggcggtc
     8101 agcgattcgt taaatatcga accatctgtc tcaaccagat tcacactaca ataaggttgt
     8161 ggtacgcgca gccagccctg aacctcgtga ccctgtttac aaagtgctgt aagccataat
     8221 tgccctaagg caccgcatcc caatacggta attttcattg ttcctcctca cccgcaacca
     8281 ctccgggtgt tcaataaggc tatcccttaa ttgtgcatgc tgttgcgact atgcacaatt
     8341 aagggatacg tcctggtgca ggactgttgg ttatttaact ttgcaggtat tatgcttcgc
     8401 atcaaaaatg aagggagagg aaaagatgcc atctttcgat attgtctctg aagttgatct
     8461 tcaggaagca cgtaacgcgg tcgataacgc gagccgcgaa gtggagtccc gttttgactt
     8521 ccgtaatgtt gaagcctcat ttgagctgaa cgacgccagc aaaaccatca aagtgttgag
     8581 cgagtccgat ttccaggtca atcagttgct ggatattctg cgtgccaagc tgctgaagcg
     8641 cggcattgaa ggcagttcac tggatgtacc ggaaaatatc gttcatagcg gtaaaacctg
     8701 gtttgtggaa gcgaaactga aacagggcat tgagagcgcg acccagaaga aaatcgtcaa
     8761 gatgatcaaa gacagcaaac tgaaagtgca ggcgcaaatt cagggtgatg agatccgcgt
     8821 aacgggtaaa tctcgtgatg atttgcaggc tgtcatggcg atggtacgtg gtggcgatct
     8881 cggtcagccg ttccagttca aaaacttccg cgattaatcg cgacaatgtg cgttttgttc
     8941 atgccgaatg cagcatgaac agctaattcg gcctacaacg atttgtaact caggaacttt
     9001 gtaggcctgg taagcgtagc gcagcaggca attcgcctcg ttttttatgc ctgacgaatt
     9061 gcctgttcaa cctcaaagcg attcgtcact ttgctgtcga ttttcacata agctgaatgt
     9121 tcttcttctg caatcaacac ttctttgacg cccgcggttt ccagcaaacg cacttttaac
     9181 gcctcgtttg cggcaatgtc cgccgggatt tcaatgcgca agctgctgac atacggcggt
     9241 tctttcatgg tactggcgac tgccagccac actgcggcca gcattgcgcc agcgagaaat
     9301 actccctgac cgtcaaacat gccgtcaatc cagccgccca gcgaaccgcc aatcgccacg
     9361 ccaagaaact ggctggtgga gtaaacaccc atcgccgtac ctttgtaacc tgctggcgac
     9421 tctttactga taagtgaagg cagaagggct tccatcaaat taaacgctac aaagaaaagc
     9481 tgcacgccga ccaccagttg ccagaactgc gtttgtgcgt tccacaacac aatttccgca
     9541 accacgatca acccaacgca gaagacaaag acttgcttca ttttgcgctt aacttcagcg
     9601 tagataatga aaggcacgac cgagccaaag gcgattagca ttgtcgccag atagaccttc
     9661 cagtgttcag ccgccgggaa ccccgcatca gccagttgtc cgggcagggc aacaaacgtc
     9721 gacatcagca aaatatgcag acacataatg ccaaagttga gtttcagcag ccgcggttcc
     9781 gccagcactt tactgaaact gcctttcacc attccggact cacgattaag tacgtgagtg
     9841 ctactgttgg gcacaaccca aatggtcaac gcaatgccgg tcgttgccag aatagcgatc
     9901 atccagaaca gcgcgtgcag cccaagtttg tgagtgatga tcgggccaag caccatcgca
     9961 atggcaaagg taatgccaaa gctcacgccg ataaacgcca ttgctttggt gcggttttgt
    10021 tcgcgcgtga gatcggaaag cagcgccata acggcagcgg caatcgcacc ggagccttgt
    10081 agcgcccggc ccagaataat tccccagatg gagtcagaga gcgcagcgat aacgctaccg
    10141 gcggcaaaca ctgccagccc accgacaatt aatggtttgc gaccaatgcg gtcggaaagc
    10201 aggccaaacg gaatctgaaa aacggcctga gtcagaccat aaataccaat ggcaataccg
    10261 attaatgctt cgctggcacc ttgcagagcc atgccgtacg tggtcagaac cggcagaacc
    10321 ataaacatgc ccagcatgcg caacgagaat acggtcccta aaccccaggt cgcgcgccgc
    10381 tcacctggcg tcattttata atcgttcatt accacctctg ttttaaattc gcgactagtg
    10441 taaagcggca aacgaatggg gtaaacatac ggatttttta gtaaatattt cgagagggat
    10501 atgtttctaa tgctaagaaa aaaggtgccg cagcaccttt ttaactgaga ggttgtatta
    10561 ccacacagca gccagcagcg tatgcgaatc cggcaccata aaatcaacgg acatcatcac
    10621 cgagagggca gtgatggcga tgatagagaa gccgaacagc ttgcgcgccc agattctgtc
    10681 atcagcaact ttataaccgc gcagggccat acctaaccac cagacgctaa ccgccgcggc
    10741 gactaccaga tatttatacc cagcgtaacc gccaagagag agcatcagcg tggcaacggc
    10801 aaaggcgatg atatacagcg tgatgtgatt cttcgccacc gaaatgcctt ttaccactgg
    10861 caataccgga atgtttgccg cctggtaatc cttaaagcgg aaaatggcga tggcatagga
    10921 gtgaggcatc tgccacagac tgaagatagc aagcaggatc gctgcgccac tatcgaactc
    10981 gccggttact gcacagtagc cgatcaccgg cggcgcagcg ccggagagcg aaccaatcaa
    11041 tgtgccgtag actgagtggc gtttcatgta caggctataa acgccgacgt aaaccacaaa
    11101 gcccatcacc cccagccagc aggccagcgg attcgcgcca aaccacagca gcataaagcc
    11161 agcaataccc agcaaggtgg cgtacaccag cgagacagca ggagagatca ggcctttaac
    11221 cagcacccga ttcttcgtcc tttccatctt tctgtcgata tccctgtcga tgtagttgtt
    11281 aaacacacaa cccgacgcca caaccagtga caccccaacc agcgtgtaga taaacagggg
    11341 ataatcaatg cggccctttg aggccagcag gaatccccca atcaccgaga tcaggttgcc
    11401 aaagatgatg cctggtttcg ttacttgcag gtattgctta aacatcataa ccgccgctct
    11461 tagtgcatca tcatgttgta gttaaggttc cacataatcc agatggagcc tacaaccagg
    11521 atagcgatga ttagcacggt gaagacaaac gccgtcatgt tccagccttc atctgatttg
    11581 gtattcatgt gcaggaagca caccagatgc accagaacct gtaccactgc cattgccagg
    11641 attgttccca gaattacggc cggagaggca gcccctgtca tcaccatcca gaacggaatc
    11701 accgtcagaa tgatcgacag gataaagcct gtcatgtagg tttttacgct gccatgggac
    11761 gcgccgctgt gatcggtaga atgactcatt acatcgcccc catcagataa acaacagtga
    11821 acacacagat ccaaaccaca tccaggaagt gccagaacag gctcaggcac atgatgcggg
    11881 tacggttagt gctggtcagg ccgcgacggg cgatttgcac catcaacacc gccatccaga
    11941 taagaccgga agtgacgtgc agaccgtgcg tgccgaccaa cgcaaagaac gctgacagga
    12001 agccgctgcg atccggaccc atgccgttaa caatcaggtg atggaattca tagatttcca
    12061 tcccgataaa tccggcacca aacaaccagg tcaacgccag ccaggagata acctggcttt
    12121 tgttgttttt gtacatggcg atagccgcca tgccgtaggt gatggagctg aacaacagca
    12181 agaaagtttc aaccagaacg aacggcagtt cgaaaatgtc cttacctgtc gggccgcctg
    12241 cggtgccgtt aaccagaacg gcataggtag caaacaagat agagaacaga atgcagtcgc
    12301 tcatcaggta gatccaaaat ccgaagattt tggttccgcc tgcatcgtgg tgcccgtgtt
    12361 cgtgcgcgtg ggcagtcgcg tgcgtcaaag tatcagttgc catttttcag ccctgcctta
    12421 gtaatctcat cgaaatgctg gttttccagt ttttcgattt ctgccaccgg cacgtagtaa
    12481 tccacgtcct cgtcgaagct tttcacgatc caggtgatga tcatgcctgc gaagccaaca
    12541 atcgccagcc accagatatg ccagatcatg gcgaaaccga agatggtgga gaaagctgca
    12601 atgacgatac ctgcaccgct gtttttcggc atatgaattt cttcatagtg gtcaggcttt
    12661 ttgtacgctt cgcctttctc tttcatttcc cagaatgcat cacgctcgtg aacgtgcggc
    12721 acaacggcaa agttatagaa cggaggcggg gaagaggttg cccactccag cgtacggcca
    12781 ccccacgggt cgccagtcag gtcacggttc tggtcgcggt cacgaataga aacgtacatc
    12841 tgaataacga ggcagagaat acccagcgca atcagtactg caccgctggc tgcaatcatc
    12901 agcatggtgt ggaactgcgg gtcaatctgc tggctcaaac gacgggtcat gcccatgaag
    12961 cccagcgcat acagcggcat aaaggcaacg aagaagccga tgatccagaa ccagaacgcg
    13021 cgtttacccc aggtttcgtt cagtttgaaa ccgaacgctt taggccacca gtaggtcatc
    13081 cctgcgaagc agccgaagac cacgccgccg atgatcacgt tatggaagtg cgcaatcagg
    13141 aacaggctgt tatgcagaac gaagtccgcg cccggtactg ccagcagcac gccagtcatc
    13201 ccgcccaccg agaaggtgac gataaaaccg atagtccaca gcatcgcaga atggaacacg
    13261 atgcggccct gatacatggt gaacagccag ttgaagatct tcaccccggt cgggatggcg
    13321 ataatcatgg tggtgatacc aaagaaggcg tttacgttcg cgcccgcacc catcgtaaag
    13381 aagtggtgca gccagacgat gaacgacagc acggtgatac agacggttgc ccataccagc
    13441 gaggtataac caaacagacg tttacgcgag aaggttgccg caatttcgga gaacacaccg
    13501 aaaacaggca ggatcaggat gtaaacttcc gggtggcccc aggcccaaat caggttgatg
    13561 tacatcatca tgttgccacc catatcgttg gtaaagaaat gggtgcccag atagcgatcc
    13621 agggtcaaca acgcgacggt aaccgtcaga attgggaagg aagcaataat cagaacgttc
    13681 gcgcacagtg atgcccaggt aaatactggc atcttgaaca tggtcatgcc cggtgcgcgc
    13741 atcttcagaa tggtaacgaa gaagttgata ccggttagcg tcgtacctat accggatagc
    13801 tggagactcc atatccagta atcgacaccg actcccggac tgtactctat tcccgatagc
    13861 ggtggatagg ccagccagcc ggtctgcgca aattcgccca cgccgagaga aacgttaacc
    13921 agaatcacac caacaacggt aaaccagaag cttaggttgt tgaggaacgg gaacgcaacg
    13981 tcacgcgcgc cgatctgcag cggaaccacc aggttcatca gaccgataac gaaaggcatc
    14041 gccacgaaga agatcataat cacgccgtgc gcggtgaaga tctgatcgta gtggtgaggt
    14101 ggcaggaagc ccgcttcgcc cgccgaggca agagcctgct ggctacgcat cataatggcg
    14161 tcagcaaaac cacgcagcaa catcacaatc gccacgatga tatacatgat accgaggcgt
    14221 ttatggtcga cggaggtcag ccactctttc cacaggtagg tccacttacc gaagtaagtg
    14281 atcaggccaa cgagcgccag acctcccaaa ataatgccag cgatcgtaac catgacgata
    14341 ggttcatgga acgggactgc atcaagtgat aattttccga acatctttat tcttcctcaa
    14401 cccctttaat gggcggattc cgcgtggctc atgtccatgc cttccatacc ttcgtgtgcg
    14461 ctgtgctcac cttctggctg ggtcatgttc atgctcttac cgtgagccat aaacttgtta
    14521 atcacatcgg caaacaagtc tggtttcacg ttggagaaat attccacctg gttgtattcg
    14581 ctaggcgcgg ccagtttttc gaacgctgcc atgtcagaca tggtgttcgg cgactgtttc
    14641 gcttttgcga cccactggtc gaatgcggcg cgatccggtg ttgcaatagc tttgaacttc
    14701 atgcctgaga agcccgggcc gctgtagctg gcggagatac cgtcataagt accgggttcg
    14761 ttggcgatca gatgcaggcg agtctgcata ccggccatgg cataaatctg gctacccaaa
    14821 cgcggaatga agaaggagtt catcacggag ttggaggtca ctttgaagta caccggagtg
    14881 ttcgccggga aagcgatttc attcacggta gcaatgccct gttccgggta gatgaagaac
    14941 catttccagt ccatggaaac cacttcgatg gtaatgggct tctcgtcgtg tgccagcggc
    15001 ttgctaggct caagagcgtg agtggttttc caggtcagta ccgcaaggaa gatgatgatt
    15061 aagataggta ccgtccagac cacagcttcc actttattgg agtgtgacca gttcgggctg
    15121 tacttagcat ctttattgct cgcacggtac ttccaggcga aaccaacagc catcaagatt
    15181 gcgggaataa cgacaatcaa catcaggcca aatgccgtca gtatcagtga acgttgctcc
    15241 agaccaatct gtcctttggg atctaacagc gcagaattac agccactgag caatacagtg
    15301 cctgcaaata atgacaacca tcccaaactt ttattgtatt tcctgagtct catttaacga
    15361 cctcaattcc acgggatctg gtggcgttta aagtgtgtgg gcattttacg ggaaggttac
    15421 attactgtaa acatgattaa attcctgtta cttggatttg gcttccaggt cacatatgtt
    15481 gcaaaacata tcaggttgat tttaagaagc tgttgca
//
LOCUS       DARNVM010000114        15395 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.114, whole genome shotgun sequence.
ACCESSION   DARNVM010000114 DARNVM010000000
VERSION     DARNVM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.114"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(117..1475)
                     /gene="plaP"
                     /locus_tag="SI621_003256"
     CDS             complement(117..1475)
                     /gene="plaP"
                     /locus_tag="SI621_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HEI4065929.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1465..1527)
                     /gene="yoeI"
                     /locus_tag="SI621_003257"
     CDS             complement(1465..1527)
                     /gene="yoeI"
                     /locus_tag="SI621_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HEI4065930.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1742..2671)
                     /locus_tag="SI621_003258"
     CDS             complement(1742..2671)
                     /locus_tag="SI621_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4065931.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKQYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASAWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2717..3541)
                     /locus_tag="SI621_003259"
     CDS             complement(2717..3541)
                     /locus_tag="SI621_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4065932.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIEGSRICNELGFEYQYPDPLVMPLE"
     gene            3968..4018
                     /gene="hisL"
                     /locus_tag="SI621_003260"
     CDS             3968..4018
                     /gene="hisL"
                     /locus_tag="SI621_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HEI4065933.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    3994..4117
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4164..5063
                     /gene="hisG"
                     /locus_tag="SI621_003261"
     CDS             4164..5063
                     /gene="hisG"
                     /locus_tag="SI621_003261"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEI4065934.1"
                     /translation="MTDNSRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5069..6373
                     /gene="hisD"
                     /locus_tag="SI621_003262"
     CDS             5069..6373
                     /gene="hisD"
                     /locus_tag="SI621_003262"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEI4065935.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTHN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKGQA"
     gene            6370..7440
                     /gene="hisC"
                     /locus_tag="SI621_003263"
     CDS             6370..7440
                     /gene="hisC"
                     /locus_tag="SI621_003263"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEI4065936.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIDNYAQYAGIKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERAVQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7440..8507
                     /gene="hisB"
                     /locus_tag="SI621_003264"
     CDS             7440..8507
                     /gene="hisB"
                     /locus_tag="SI621_003264"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEI4065937.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQINVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8507..9097
                     /gene="hisH"
                     /locus_tag="SI621_003265"
     CDS             8507..9097
                     /gene="hisH"
                     /locus_tag="SI621_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEI4065938.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9097..9834
                     /gene="hisA"
                     /locus_tag="SI621_003266"
     CDS             9097..9834
                     /gene="hisA"
                     /locus_tag="SI621_003266"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEI4065939.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPAGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            9816..10592
                     /gene="hisF"
                     /locus_tag="SI621_003267"
     CDS             9816..10592
                     /gene="hisF"
                     /locus_tag="SI621_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEI4065940.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10586..11197
                     /locus_tag="SI621_003268"
     CDS             10586..11197
                     /locus_tag="SI621_003268"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEI4065941.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11383..12363)
                     /gene="wzzB"
                     /locus_tag="SI621_003269"
     CDS             complement(11383..12363)
                     /gene="wzzB"
                     /locus_tag="SI621_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HEI4065942.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVVIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEREKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12509..13675)
                     /gene="ugd"
                     /locus_tag="SI621_003270"
     CDS             complement(12509..13675)
                     /gene="ugd"
                     /locus_tag="SI621_003270"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEI4065943.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPMLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(13924..15330)
                     /gene="gndA"
                     /locus_tag="SI621_003271"
     CDS             complement(13924..15330)
                     /gene="gndA"
                     /locus_tag="SI621_003271"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEI4065944.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         3907 a         3951 c         3896 g         3641 t
ORIGIN      
        1 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
       61 taaaaaaacc ggagccaaat attcattcga ctccggttga ttattagaaa agatgattat
      121 gctacgtctt cgtactgcgg aaccgggtta cggaagctct tggttacgca tgccaggtaa
      181 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
      241 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
      301 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaacg
      361 aaggtgaacg ccaccaacgc accaaagtta atcagcgccg ttgccattac caggtcaaag
      421 ttgattgcca gcagggcaat cgcgccaacc aggatgatat tcatcgccgg agtacgccat
      481 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
      541 atcagacgcg caacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
      601 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
      661 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
      721 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
      781 cgctctgcat ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc ggtaaaggag
      841 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
      901 ggacgagtgc tcgccagcgt acccgctcct tcaccttcaa atacgccata aacaaccatg
      961 cccaggatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gaagttcgct
     1021 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     1081 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     1141 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     1201 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcggacc
     1261 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     1321 ggcacatgac catccgtgag gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc
     1381 tgcatatagg caagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     1441 acgcgagagg tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac
     1501 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     1561 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     1621 agcaaaaaat aaccgacgct gatgaaacgt cggtttttag tcattttttg acagcggcgc
     1681 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     1741 tttaaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     1801 ctaaaatctc gtctcgccac caggcgctgg caagtcctgc ggtctgttcg ttccagccaa
     1861 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     1921 cgagaaaacg ttgcgccaga taacggggta aatagccgca gccgaggccg ctaatttgca
     1981 gctccagctt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgcg
     2041 aggccgtaga agcgccggcc tgcgcagtat cccctaccac aatcgcccta tattgcttaa
     2101 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatggggc gcgaccgcaa
     2161 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     2221 gcatcgcgcc aacaataata tccgctcgcc cctgagtcag ggcatcccat gaaccaccga
     2281 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     2341 gtggcgcaag cagagaaaaa gggaaggtgt cgtccacgcc aatcaccagc tcgttttccc
     2401 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     2461 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctgcgat
     2521 caagcaattg aatattaagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     2581 gcgtcttgta gagtttggcc gacgccgccg caaaactgcc ttctttttct aaggcatcga
     2641 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     2701 ctgctggcgt gatcgtttac tccagcggca ttaccagcgg atcgggatac tggtattcaa
     2761 atcccagttc attacaaatc cgactgccct caataatctt accttttccg ctgtccagac
     2821 tatttctgaa ctgcggaggt tccagtccca gtaaacgggc catctgcgga tagaaaacat
     2881 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     2941 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     3001 catcaggggc ggtttttccg gcaaagaaac gtccgggatg acgtcccggt ccgaccagac
     3061 ccgcaagacg tagaatatcg accgaagttc cgggtaaatt gtgcagccag tcttcgagtt
     3121 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtggtttct ttcaccgtgc
     3181 cttgcgcgtc gccatagaca gatgttgagc tggtgaaaat aatgcgggga atacgatggg
     3241 ccagcgcgct atctactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     3301 tacgacgtgc cggaagcgta atgaccagcg catcggcatc catcagggca tccagatcgt
     3361 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     3421 gggccgcttc gacaccatct tgtgtggttt tactcccggt aacttgccag cctcgcgctg
     3481 aaagtgacat cgccagcggc atgcctaacc accctaaccc gacaattgcg acctttttca
     3541 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     3601 attcattcta tgaatgaaat cgaaactgtt agcaaatata caattgtctg atgtgctccg
     3661 cttatcagtc ctacaaaaac atcaatatat tgaatttagg caatatttgt agacctgata
     3721 aggcattcac gccgcatccg gcaggaacaa agcccacttt agttacagaa cacaatgttc
     3781 ttgagggttt taaagacata ttttcacgat tacttttcta acgtagtcac tccaggcccc
     3841 ctcctttccc cgctcattca ttaaacaaat ccattgccat aaaatatata aaaaagccct
     3901 tgctttctaa cgtgaaagtg gtttaggtta aaagacatca gttgaataaa cattcacaga
     3961 gacttttatg acacgcgttc aatttaaaca ccaccatcat caccatcatc ctgactagtc
     4021 tttcaggcga tgtgtgctgg aagacattca gatcttccag tggtgcatga acgcatgaga
     4081 aagcccccgg aagatcatct tccgggggct tttttattgc gcggttgata acggttcaga
     4141 caggtttaaa gaggaataag aaaatgacag acaactctcg tttacgcata gctatgcaga
     4201 aatccggccg tttaagtgat gactcacgcg aattactggc gcgctgtggc attaaaatca
     4261 atcttcacac ccagcgcctg attgcgatgg cagaaaacat gccgattgat attctgcgcg
     4321 tgcgtgacga tgacattccc ggtctggtaa tggacggcgt ggtagacctt gggattatcg
     4381 gcgaaaacgt gctggaagaa gagctgctta accgccgcgc ccagggtgaa gatccgcgct
     4441 actttaccct gcgtcgtctg gatttcggcg gctgccgcct ttcgctggca acgccggttg
     4501 atgaagcctg ggacggtccg ctctccttaa acggtaaacg tatcgccacc tcttatcctc
     4561 acctgctcaa gcgttatctc gaccagaaag gtatctcttt taaatcctgc ttactgaacg
     4621 gttctgttga agtcgccccg cgtgccggac tggcggatgc gatttgcgat ctggtttcca
     4681 ccggtgccac gctggaagct aacggcctgc gcgaagtcga agttatctac cgctcgaaag
     4741 cttgcctgat ccagcgcgat ggcgaaatgg aagaatctaa acagcaactg atcgacaagc
     4801 tgctgacccg tattcagggc gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg
     4861 caccgaccga acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacta
     4921 ttctgccgct ggcaggtgac caacagcgcg tagcgatgca catggtcagc agcgaaaccc
     4981 tgttctggga aacgatggaa aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc
     5041 cgattgagaa gatgatggag tgatcgccat gagctttaac acaatcattg actggaatag
     5101 ctgtactgcg gagcaacaac gccagctgtt aatgcgcccg gcgatctccg cctctgaaag
     5161 cattacccgc actgttaacg atattctcga taacgtgaaa acgcgtggcg atgaggcctt
     5221 gcgggaatac agcgcgaagt ttgataaaac cacggttacc gcgctgaagg tgtctgctga
     5281 tgagatcgcc gccgccagcg aacgcctgag cgacgagcta aaacaggcga tggcggtggc
     5341 agtaaagaat attgaaacct tccacactgc gcaaaaactg ccgccggtag atgtagaaac
     5401 gcagccaggc gtacgttgcc agcaagtcac gcgtccggta gcttcagttg ggttgtatat
     5461 tcctggcggc tccgccccgc tcttctcaac ggtattaatg ctggcgactc cggcgcgtat
     5521 tgcgggctgt aaaaaagtgg tgctgtgctc accgccgccg atagccgatg agatacttta
     5581 cgcggcgcag ctgtgcggtg tgcaggacgt gtttaacgtc ggcggtgcac aggccattgc
     5641 cgcgctggcg tttggtacag aatccgtgcc gaaagtggac aaaatcttcg ggccaggtaa
     5701 cgcctttgtc accgaggcaa aacgtcaggt gagccagcgt ctggacggtg cggcgatcga
     5761 tatgcccgca ggcccgtcgg aagtgctggt gattgctgac agcggcgcta cgccggattt
     5821 cgtggcttct gatttgcttt ctcaggctga acacggcccg gactcacagg tgattttact
     5881 gacgcccgac gccgatatgg cgcgtcgcgt tgccgaggct gtcgaacgcc aactggcaga
     5941 actgccgcga gctgaaaccg cccgccaggc actgaacgcc agccgcctga tcgtgactaa
     6001 agatttagcg cagtgcgtag agatctccaa ccagtacggc ccggagcacc tgatcattca
     6061 gacccacaac gcccgcgaac tggtcgatgg catcaccagc gccggttcgg tatttcttgg
     6121 tgactggtca ccggaatcgg caggcgacta tgcctccggc accaaccacg ttctgccgac
     6181 ttacggttac accgccacct gttccagcct cgggctggcg gatttccaga agcgcatgac
     6241 cgtgcaggaa ctgtcgaaag taggtttctc cgctctggcg tcgaccattg aaacactggc
     6301 cgccgccgag cgcctgaccg cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa
     6361 ggggcaagca tgagcaccgt gactattacc gatttagcgc gtgaaaacgt ccgcaacctg
     6421 acgccgtatc agtcggcgcg tcgtctgggc ggtaacggcg acgtctggct gaacgccaac
     6481 gaatacccca cagccgtgga gtttcagctt actcagcaaa cgctcaaccg ctacccggaa
     6541 tgtcagccga aagcggtgat tgacaattac gcgcagtatg caggcataaa accggagcag
     6601 gtgctggtca gccgtggcgc ggacgaaggt attgaactac tgattcgcgc tttttgcgaa
     6661 ccgggtaaag acgccatcct ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct
     6721 gaaaccattg gcgtcgagtg ccgcacagtg ccgacgctgg acaactggca actggacttg
     6781 cagggcattt ccgacaagct ggacggcgta aaagtggtct atgtttgcag ccccaacaac
     6841 ccgacagggc aactgatcaa tccgcaggat tttcgcaccc tgctggagtt aacccgcggt
     6901 aaggcgattg tggttgccga tgaagcctat atcgagtttt gcccacaggc atcgctggct
     6961 ggctggctga cggaatatcc gcacctggct attttgcgca cactgtcgaa agcttttgct
     7021 ctggcgggcc ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa cctgctgatg
     7081 aaagtgatcg ccccctaccc gctctcgacg ccggttgccg acattgcggc ccaggcgtta
     7141 agcccgcagg gaatcgtcgc catgcgtgaa cgggcagtgc aaattattgc tgaacgcgaa
     7201 tacctgattg ccgcattgaa agaaatcccc tgcgtggagc aggttttcga ctccgaaacc
     7261 aactacattc tggcgcgctt taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag
     7321 ggcattatct tacgtgatca gaataaacaa ccctctttaa gcggctgcct gcgaattacc
     7381 gtcggaaccc gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga gcaagtttaa
     7441 tgagtcagaa gtatcttttt atcgatcgcg atggaaccct gattagcgaa ccgccgagtg
     7501 attttcaggt ggaccgtttt gacaaactcg cctttgaacc gggcgtgatc ccggagctac
     7561 tgaagctgca aaaagcgggc tacaagctgg tgatgatcac taatcaggat ggtctgggaa
     7621 cacaaagttt cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg cagatcttca
     7681 cctcacaagg cgtgcagttt gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt
     7741 gcgactgccg taagccgaaa gtaaaactgg tggagcgtta tctggctgag caagcgatgg
     7801 atcgcgctaa cagttatgtg attggcgatc gagcgaccga cattcaactg gcggaaaaca
     7861 tgggcattaa tggtttacgc tacgaccgcg aagccctgaa ctggccaatg attggcgagc
     7921 aactcactaa acgagaccgt tacgcccatg tagtgcgtaa caccaaagag acgcaaatta
     7981 acgtccaggt gtggctggat cgcgaaggcg gcagcaagat taacaccggc gttggcttct
     8041 ttgatcacat gctggatcag atcgctaccc acggtggttt ccgtatggaa atcaacgtta
     8101 aaggcgacct ctatatcgac gatcaccaca ctgtcgaaga taccggtctg gcgctgggcg
     8161 aagcgttaaa aatagctctt ggcgacaaac gcggtatttg ccgcttcggt tttgtgctgc
     8221 cgatggacga atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg cacctggaat
     8281 ataaagctga atttacctac cagcgtgtgg gcgatctcag cactgaaatg atcgagcact
     8341 tcttccgctc actctcttac accatgggcg tgacgttgca cctgaaaacc aaaggtaaaa
     8401 acgatcatca ccgtgtagag agcctgttca aagcttttgg tcgcaccctg cgccaggcca
     8461 tccgcgtgga aggcgacacc ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat
     8521 ccttgatacc ggctgcgcca atttgaactc ggtgaagtca gccattgcgc gtcacggtta
     8581 tgaacccaaa gtcagccgtg acccggacgt cgtgttgctg gccgataaac tgtttttacc
     8641 cggcgttggc accgcgcaag cggcgatgga ccaggtgcgc gagcgcgagc tgtttgatct
     8701 catcaaagcc tgtacccaac cggtgctggg catctgctta gggatgcaac tgctggggcg
     8761 gcgcagcgaa gagagcaacg gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa
     8821 aatgaccgac tttggtctgc cactgccgca tatgggctgg aaccgcgttt acccgcaggc
     8881 aggcaaccgc ctgtttcagg gaattgaaga cggtgcgtac ttttactttg ttcacagtta
     8941 cgcaatgccg gtcaatccgt ggaccatcgc ccagtgtaat tacggcgaac cgttcaccgc
     9001 ggcggtacaa aaagataact tctacggcgt gcagttccac ccggagcgtt ctggtgccgc
     9061 tggcgctaag ttgctgaaaa acttcctgga gatgtgatga ttattccggc attagattta
     9121 atcgacggca ccgtggtgcg tctccatcag ggcgattacg gcaaacaacg cgattacggt
     9181 aacgacccgc tgccgcgctt acaggattac gctgcgcaag gtgccgaagt gttgcatctg
     9241 gtggatctga ccggggcaaa agatccggct aaacgtcaaa tcccactgat taaaaccctg
     9301 gtcgcgggcg ttaacgttcc ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg
     9361 gcggcgttac tggaagcagg cgttgctcgc gtggtggttg gctccaccgc ggtgaaatca
     9421 ccagaaatgg tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt gctggcgctg
     9481 gatgtccgta ttgacgagca aggcaacaaa caggtggcag tcagcggctg gcaagagaac
     9541 tcgggcgttt cgctggaaca actggtggaa acctatctgc ccgccggcct gaaacatgtg
     9601 ctgtgtaccg atatctcgcg cgacggcacg ctggcaggct ctaacgtctc tttatatgaa
     9661 gaagtgtgcg ccagatatcc gcaagtggca tttcagtcct ccggcggcat tggcgacatt
     9721 aacgatgttg ctgccctgcg aggtactggc gtgcgcggcg taatagttgg tcgggcatta
     9781 ctggaaggta aattcaccgt gaaggaggct atcgcatgct ggcaaaacgc ataattccat
     9841 gtctcgacgt tcgtgatggt caggtggtga aaggcgtaca gtttcgcaac catgaaatca
     9901 ttggcgatat cgtgccactg gcaaaacgct acgccgaaga aggtgcagat gaactggtgt
     9961 tctacgatat caccgcttcc agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg
    10021 tggcggaagt aatcgacatt ccgttttgtg tggcgggtgg gattaagtct ctggaagatg
    10081 ccgcgaaaat tctttccttt ggcgcggata aaatttccat caactctcct gcgctggcgg
    10141 acccgacgtt aattactcgc ctggcggatc gctttggcgt gcagtgtatt gtggtcggta
    10201 ttgatacctg gtacgacgcc gaaaccggta aatatcatgt gaatcaatat accggcgatg
    10261 aaagccgcac ccgcgttact cagtgggaaa ctctcgactg ggtacaggaa gtgcaaaaac
    10321 gcggtgccgg agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt aacggttacg
    10381 acctcgagca gctgaaaaaa gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg
    10441 gcgcaggcac catggaacac tttctcgaag ccttccgcga tgccgacgtt gacggcgcgc
    10501 tggcagcttc tgtattccac aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg
    10561 caacacaggg cgtggagatc aggatatgtt aacagaacaa caacgtcgcg aactggactg
    10621 ggaaaaaacc gacggtctga tgccggtgat tgtgcaacac gcggtatctg gcgaagtgtt
    10681 aatgctgggc tacatgaacc cggaagcctt agacaaaacc ctcgaaagcg gtaaagtcac
    10741 cttcttctcg cgcactaaac agcgactgtg gaccaaaggc gaaacctcgg gcaatttcct
    10801 caatgtggtg agcattgcgc cggactgcga caacgacacg ttactggtgc tggcgaatcc
    10861 catcgggcca acctgccaca aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg
    10921 gctgttcctg tatcaactgg aacaactgct cgccgaacgt aaatctgccg acccggaaac
    10981 ctcctacacc gccaaactgt atgccagcgg caccaaacgc attgcgcaga aagtgggcga
    11041 agaaggcgtg gaaaccgcac tggcagcaac ggtacatgac cgctttgaac tgaccaacga
    11101 ggcgtctgat ttgatgtatc acctgctggt gctgttgcag gatcaggatc tggatttaac
    11161 aacggtaatt gagaacctgc gtaaacggca tcagtgagtg gcgtgctgag caggtgtgat
    11221 gttgttgtca cacccggagc cattacccga tgcgcaagct tatcgtgcct acacattatc
    11281 tcaacacacg taggccggat aagatgcgcc agcatcgcat ccggcaaaaa aaacgggcat
    11341 ggtgtcacca ccctgcccgt ttctcttaaa tgcacaataa tattatttcg cgttgtaatt
    11401 acgcagcgcg ttacgcccaa gcacaatccc cgcgccaacc atgccgccca gcagcactgc
    11461 cagaatcaag gtaattgcct ttttcggact atcgcgacga ataggtaacg tcggtttcat
    11521 cacataacgg taagcatgaa tatcaagatc atcaaccttt aagctttcga tatccagcag
    11581 gttttgacga gtctgatagt agtttggcga gaacaccaac ggacgggtcg cctcgtgctt
    11641 aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgtg tgatatcttc
    11701 gccagtctgt tgaatctgcg gttttgtcac ctgcgcctga ttcgcatact gcaacgcttc
    11761 ctgaatctga cggatacgca gatctttctg ctcctgcgcg actacttcct gcgttcttaa
    11821 agagtcctgc aagtttttcc gtcccagagc aatgttgtct ttgagatcct tttctagctc
    11881 ttgattcact ttatcgtcaa cttgctgaat gtattgggcc aacttcattt gtgcgccctc
    11941 tgcagtttgc ccaacataag aaacagtcaa tggtaattgc tggttcttaa cagaaggttc
    12001 gatggtaagt ttttctcgtt cttcctgatt atccagcgtt tctgctaatg cagagaaggc
    12061 agaactgaag cgaccaatta acgtctcctg caaatccgat actttcggtg cagcctgacc
    12121 atagataaca ttcatggcat tgttatagcc agcaatttgc cccacgtcgg gctgagtgat
    12181 aattgctgtt gacgtccatt tctccttcgc tactgccaaa tatccaatag ccagggcaat
    12241 aaccacaatg acggaaatga tgattgtcat cttgccacgc cacaactgca ctagtaaatc
    12301 aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat tttctactct
    12361 catcattacc ctaactgacg gaaatttcca agtccgcata gtttagcgat aatcctcgtg
    12421 aatgctatag gataagtgat aaaaataatg aattgttgtg taagtaacaa aagacaatca
    12481 gggcgtaaat agccctgata acaagatgtt agtcgctgcc aaagagatcg cgggtgtata
    12541 ccttatctgc cacatcctta agctcttctg ccattcggtt agagataatg acgtcggctt
    12601 gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt gaagaatgag tcttctttca
    12661 tcactggctc gtagatgatc acttcaacac ctttcgcctt gatacgtttc ataatcccct
    12721 gaatagaaga cgcacggaag ttatctgaac cgctcttcat aatcagacga taaataccca
    12781 ccacttgcgg cttgcgtgac aaaatggcat cggcaataaa atctttacgc gtgcggttag
    12841 catcgacaat tgccgagatc aggttattcg gcacagactg gtagttcgcc agtaactgct
    12901 tggtatcttt cggcagacaa taaccaccat aaccaaacga cggattgttg taatggttgc
    12961 caatacgtgg gtcgagacaa acgccttcga ttatttgacg ggaattcaga cctaaacttt
    13021 ctgcatagct atccagttcg ttaaagtacg ccacgcgcat cgccaggtag gtgtttgcaa
    13081 aaagtttaat cgcttctgct tcagtggagt cggtaaacag catcgggata ttttgcttaa
    13141 tcgcgccttc ctgtaacaga gcagcgaaac gttctgcgcg ttctgaacgc tcaccgatga
    13201 caatacgtga aggatggaga ttatcgtaaa gggctttacc ctcacggaga aattccgggg
    13261 agaatataat attttcagtg cgatatttct tatgcatcgc tgcggtaaaa ccaacgggaa
    13321 ccgttgattt gatgaccata accgcataag gatttatctc aactacgtct ttaattactg
    13381 attctacact ggatgtattg aaataattag ttttaggatc atagtcggtt ggagtggcga
    13441 tgatgacata atcagcatcc cggtaggctt catttttatc taatgtggca ttaaagtgta
    13501 ttttatctga ttgcaaaaac tgctgaattt ccttatcaac aataggagat atccgatcat
    13561 tcagcatagc aacgcgtgac ggtaaaatat ctaatgccac aacctcatga ttttgtgcga
    13621 ttagaagccc gtttgacaag cctacatagc cagtaccgga aatggtgatt ttcatttcat
    13681 gctctcagaa ttaacttaac tgtgaatcat gatgttttta gcatcctgat aagagctaaa
    13741 agttttaacg ctacgttgtt cgatggtcaa cgcaacgaaa aacaattcag attttcttta
    13801 taagaatagc tgatatttat taagttaata ttaagcagtg aaatttagtc tataagatat
    13861 ttggcaaaaa aaagcccggt gcaatatacg ccgggcctca attttattgt tggttaaatc
    13921 agattaatcc agccattcgg tatggaacac accttcttta tcaatacgct tataagtatg
    13981 cgcaccaaaa tagtcacgct gtgcctggat caggttcgca ggcagaacag cagcacggta
    14041 gctgtcgtaa taggcaaccg ctgcggagaa ggtcggaacc ggaataccgt tctgtactgc
    14101 ataagcaacg acatcacgca gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg
    14161 agccagcagc aggttagcga tctgcggatt ttcggcataa gcatcggtga ttttctgcag
    14221 gaactgcgca cggatgatgc agccagcacg gaaaatcttc gcgatttcgc cgtagttcag
    14281 atcccagttg tactcttcag acgcagcacg cagctgagag aagccctggg cgtaagaaac
    14341 gattttgccc agatacagcg cacgacgaac tttttcgatg aactcagcct tgtcgcctgc
    14401 tggctgtgct tgcggaccag agagaacttt agatgcggca acacgctgat ctttcagaga
    14461 agagatataa cgtgcaaaca cagactcggt aatcagcgac agcggttcgc cgagatccag
    14521 cgcgctctgg ctggtccatt taccggtacc tttgttagcc gcttcatcca ggatcacatc
    14581 aaccaggtag ttaccgtctt catctttttt ggtgaagata tctttggtga tgtcgatcag
    14641 gtaactgctc agttcaccgt tattccactc ggtaaaggtc tgcgccagtt cttcgttggt
    14701 gaggttcagg ccacctttaa gcagagaata ggcttcagca atcagttgca tatcaccgta
    14761 ttcgataccg ttgtgaacca tcttcacata atggcctgcg ccatcggcac caatataggt
    14821 aacgcatggc tccccgtctt cagctactgc ggcgattttg gtcaggattg gtgcaaccag
    14881 ttcataggct tctttctgcc caccaggcat aatagaagga cctttcagcg cgccttcttc
    14941 accaccggag acaccggtac caatgaagtt aaagccttcg gcagaaagtt cacggttacg
    15001 acgaatggtg tcctggaaga aggtattgcc accatcaatg atgatgtcac ctttatcgag
    15061 atatggtttg agggaatcaa tagcagcatc cgtgcctgca cctgctttca ccattaacag
    15121 gatgcgacga ggcgtttcca gagattcaac aaactctttc accgtatagt aaggaaccag
    15181 tttcttgcct ggattttcgg caatcacttc ttccgtcttt tcacgggaac ggttgaaaat
    15241 agagacggta taaccacgac tttcgatgtt gagcgcgagg ttgcgcccca tcactgccat
    15301 accgacgacg ccgatctgtt gctttgacat tgtttactcc tgtcaggggt gatcaccgcg
    15361 gggttgcggc ttattgaaga taaagaatag cttaa
//
LOCUS       DARNVM010000115        14949 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.115, whole genome shotgun sequence.
ACCESSION   DARNVM010000115 DARNVM010000000
VERSION     DARNVM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.115"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..546
                     /locus_tag="SI621_003272"
     CDS             <1..546
                     /locus_tag="SI621_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065945.1"
                     /translation="NSSDKSYKTSSEESIKSQLYSDQKKYTDLLLHSEKNTEWYKYAS
                     SVERYDKFKNSSKEIKNTHKQIVQAQKKLNQMKYINKLSGELIDIADKKLAPLINDSF
                     SYTRDFFAYSKQENNIFTFDNSKFEDPKEKKGLMVQHSNGQLVITGEYYPEGVQIAFT
                     QEQYNKLIRYINIFFTFPKCE"
     gene            complement(767..1843)
                     /gene="glpQ"
                     /locus_tag="SI621_003273"
     CDS             complement(767..1843)
                     /gene="glpQ"
                     /locus_tag="SI621_003273"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEI4065946.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1848..3206)
                     /gene="glpT"
                     /locus_tag="SI621_003274"
     CDS             complement(1848..3206)
                     /gene="glpT"
                     /locus_tag="SI621_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HEI4065947.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            3479..5107
                     /gene="glpA"
                     /locus_tag="SI621_003275"
     CDS             3479..5107
                     /gene="glpA"
                     /locus_tag="SI621_003275"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HEI4065948.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYS
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5097..6356
                     /gene="glpB"
                     /locus_tag="SI621_003276"
     CDS             5097..6356
                     /gene="glpB"
                     /locus_tag="SI621_003276"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HEI4065949.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDSQAVTDIHHGLESLCQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            6353..7543
                     /gene="glpC"
                     /locus_tag="SI621_003277"
     CDS             6353..7543
                     /gene="glpC"
                     /locus_tag="SI621_003277"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HEI4065950.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7736..8638
                     /locus_tag="SI621_003278"
     CDS             7736..8638
                     /locus_tag="SI621_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEI4065951.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMREQAIKIALRMLE
                     QGIDRDLVLAATQLSEADLAANNH"
     gene            complement(8679..9482)
                     /gene="yfaU"
                     /locus_tag="SI621_003279"
     CDS             complement(8679..9482)
                     /gene="yfaU"
                     /locus_tag="SI621_003279"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HEI4065952.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(9499..10788)
                     /locus_tag="SI621_003280"
                     /pseudo
     CDS             complement(9499..10788)
                     /locus_tag="SI621_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10845..12050)
                     /gene="rhmD"
                     /locus_tag="SI621_003281"
     CDS             complement(10845..12050)
                     /gene="rhmD"
                     /locus_tag="SI621_003281"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEI4065953.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIHKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(12065..12847)
                     /locus_tag="SI621_003282"
     CDS             complement(12065..12847)
                     /locus_tag="SI621_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEI4065954.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(13067..14269)
                     /locus_tag="SI621_003283"
     CDS             complement(13067..14269)
                     /locus_tag="SI621_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HEI4065955.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATARGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TSGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(14369..14911)
                     /locus_tag="SI621_003284"
     CDS             complement(14369..14911)
                     /locus_tag="SI621_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4065956.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
BASE COUNT         3812 a         4072 c         3653 g         3412 t
ORIGIN      
        1 aacagctctg ataaatctta taaaaccagc agtgaggaaa gtattaaaag tcaactttac
       61 agcgatcaaa aaaaatatac agacctgcta ctacattctg agaaaaatac agaatggtac
      121 aaatacgcat cctccgtgga acgctatgat aaattcaaga attcttctaa ggaaattaaa
      181 aatacccaca agcaaattgt acaagcgcag aaaaaattga accaaatgaa atacatcaat
      241 aaattaagtg gtgagcttat cgatatcgcc gacaaaaagt tagcaccatt aataaatgat
      301 agtttcagct atacacgaga tttctttgct tactcaaaac aggaaaataa catcttcacg
      361 tttgataatt ctaagtttga agatccaaag gaaaaaaaag gtttaatggt acaacacagc
      421 aatggacaac tggttattac tggtgaatat tatcctgaag gtgtgcaaat agcattcact
      481 caagaacaat ataataaatt aataagatac attaatattt tctttacttt tccgaaatgc
      541 gaataatact ttacagtata aactgcaata caatttatta ttgaacccaa ttcatactgt
      601 ttaaggaata acaatgccca gtgttagctt tcttaacgaa tgagttttac gcaaatatgt
      661 tggcatcttt tttaacagga tgcaccggtt aatgaataga acaaccggga gtagcattat
      721 ttcgctttag cagaggatgg cgcatgaacg ccatcctgca atagcattac tctttattaa
      781 ggaattttac tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat
      841 acagagcatc atataactga ttcacatcag tagtgtattc aggcagctta tctgaccgca
      901 cggtgtaagg atgcactacc agcttgttct gctgagcatc ttgcaccatg ccagtgagtt
      961 tgatattacc tggctgcgat gtctcctcaa tcaacatatg ataatccgga ccaataccat
     1021 cagcatattc cgccacctgt ttcatggccc ccggcttaaa catccagtcg tagttgtaat
     1081 taacccagct tccgtccggc tgtttctgct gagtttcgtt ccagtcggta taggcaatca
     1141 gctgtaccag attgaggtcc atgcccattt taggttccag ctcattctta atacgcttca
     1201 gttcatcggc atcaaaacat tgcaaataaa ctttgtcgtc cttaccgctg taaccatatt
     1261 tcttcagcac ttccagcgtt ttagccgcaa tatccttccc ttcctgatga tggaaccacg
     1321 gcgctttaat ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa
     1381 caaattcaat ctcttcttcg aaagtgtgta cccggaagtc agatttaccc atcgggaaac
     1441 gccccggata agtctgtact ttcttaccgt tttcaatatc gaaaccttcg gtaaatttca
     1501 gcgacttaat ttcatcgagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc
     1561 gatccgggaa acgatcggca acatcagtaa cacgatcgag ataatggtca tgcaggacaa
     1621 ccagatggtc gtccttcgtc atcaccaaat cctgttccag ataatccgct ccctgcgcat
     1681 acgccatcgc ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg
     1741 cgatgactat tttttcgttg ctatccgccg ccattgcact gcttcccatg atcattccac
     1801 tcatcatgat cgccatgcta aggtttttta gcgtcaattt catgccatta gcctccgttg
     1861 cgtttttgca gtaattcctg atggcgacgt ttttcgccaa tcatcacaac aatcaacaag
     1921 ataaccgcga gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag
     1981 tccacggtgt agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc
     2041 ccggtaaagc ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga
     2101 ccgatcagca tcacagggcc gtagatcagg aagccgataa caatcataca aatcatatct
     2161 acggttgggt tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc
     2221 ataaagaaga cgccggtggc cccacggttg ccacggaaga ctttatccga catccagccg
     2281 cacagcaggg tgcccggaat gcccgcatac tcatagaaga agtaggccca ggaggattta
     2341 tccagcgcga agtgtttcac ctctttcaga taagtcggtg accagtcgag gatgccgtaa
     2401 cgcagcagat aaacgaacac gttggcgata gcgatgtacc acagcagttt gttcggcagc
     2461 acgtactgca tgaagatttg cttcgccgtc agctcctgtt ccgctttttc gttatagtcg
     2521 tccggataat catttttgta ctcttcgatc ggcggcaggc cacaggattg cggggtatcg
     2581 cgcatcatcg caaaggcgaa cagtgccacc agaatggcgc agaaagcggg catatagagc
     2641 gccgcgtgcc agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca
     2701 ccaccgacgt tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac
     2761 caccagtgca ccatagtacg accgcacggc ggccatccca tcccctggaa ccaaccgcag
     2821 aggaacagca gcacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata
     2881 aacaacatca ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag
     2941 cgatccgata ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa
     3001 agggcaaaac ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcgagc
     3061 gcaaagttct tacgaaccaa atagtaagca gcatagccaa agaatatccc caggaaaatt
     3121 tgccagcgca atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttatgt
     3181 ggcgctggtt taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga
     3241 ttaacagcct gattcagtgg gagaacctgc cgtttcttga gttgccgcga tgttaagaaa
     3301 acattcataa attaaatgtg aattaccgca cacattatta aatacgatgg ttgaaatgtt
     3361 cagaatgacg catgaaatca cgtttcactt tcgaattatg agcgaatatg cgcgaaatca
     3421 aacaattcat gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat
     3481 gaaaactcgc gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc
     3541 cgggattgcc cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga
     3601 catcgcaacc ggtgccaccg ggcgtaacca cggcctgctg cacagtggtg cgcgctatgc
     3661 ggtaaccgat gcggaatcgg cccgcgaatg tattagtgaa aaccagatcc tgaaacgcat
     3721 tgcacgtcac tgcgttgaac cgaccaacgg cctgtttatc accctgccgg aagatgacct
     3781 ctccttccag gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat
     3841 agacccgcag caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt
     3901 gaaagttccg gatggcaccg ttgatccatt tcgtctgacc gcagcgaaca tgctcgatgc
     3961 caaagaacac ggtgcggtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg
     4021 cgcgacggtg tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaattc aggcacttca
     4081 tgcgcctgtc gtagttaatg ccgccgggat ttgggggcaa cacattgccg aatatgccga
     4141 tctgcgcatt cgcatgttcc cggcgaaagg atcgctgctg attatggatc accgcattaa
     4201 tcagcatgtg atcaaccgct gccgcaaacc ttccgacgcc gatattctgg tgcctggcga
     4261 taccatttcg ctgattggca ccacctcttt acgtattgat tacagcgaga ttgacgataa
     4321 tcgagtgacg gcagaagaag ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt
     4381 gatggcgaaa acacgcattt tgcgggccta ttctggcgtg cgtccgctgg ttgccagcga
     4441 tgacgacccg agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg
     4501 cgatggtctc gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat
     4561 ggcggaatgg gctactgacg cggtgtgtcg caagctgggc aacacgcgcc cctgtaccac
     4621 tgccgacacg ccgttgcctg gctcacagga accggcagaa gtcacgctgc gcaaagtgat
     4681 ctccctgccc gctccgctgc gcggttccgc agtttaccgg cacggcgatc gcacaccagc
     4741 ctggctgagc gaaggccgcc tgcaccgcag cctggtatgt gagtgcgaag cggtaactgc
     4801 gggtgaagtg cagtacgcag tagaaaattt aaacgttaac agcctgctgg atttacgccg
     4861 tcgtacccgt gtagggatgg gtacctgcca gggcgaactc tgcgcctgcc gggctgccgg
     4921 actgctgcaa cgttttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt
     4981 ccttaacgaa cgctggaaag gcgtgcaacc catagcatgg ggagacgcac tgcgcgaaag
     5041 cgaatttacc cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc
     5101 gctttgatac tgtcattatg ggcggcggcc tcgccggctt actctgtggc ctgcaactgc
     5161 aaaaacacgg cctgcgctgt gccattgtca cgcgtggtca aagcgcgctg catttctcat
     5221 ccggttcgct ggatttgctg agccatctgc cagatagtca agctgtgaca gacattcacc
     5281 acgggctgga atcgttatgt cagcaggccc ctgcccatcc ttactctctt ctcgggccac
     5341 aacgcgtact cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat
     5401 tgcagggcag cgtagaactg gctcaccagc ggattacgcc gctcggcact ctgcgttcta
     5461 cctggctaag ttcgccagaa gtccccgtct ggccactgcc cgcgaagaaa atatgtgtag
     5521 tgggaattag cggcctgatg gattttcagg ctcaccttgc ggcagcttcg ttgcgtgaac
     5581 tcggccttgc cgttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata
     5641 acgccaccga gttccgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc
     5701 cgctgttact tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg
     5761 cctgcttcgg cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt
     5821 cactgatgct tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt
     5881 tacagcgcca gtttgtgcgc cagggtggcg tgtggatgcc aggcgatgaa gtgaaaaaag
     5941 tgacctgtaa aaatggcgta gtgaatgaaa tctggacgcg caatcacgcc gatatcccgc
     6001 tgcgcccacg atttgcagtg cttgcgagcg gcagtttctt tagcggcgga ctggtggcag
     6061 aacgtgacgg cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg
     6121 gtgaatggta taagggcgat ttttttgcgc cgcaaccgtg gcagctgttc ggtgtaacca
     6181 ctgatgaggc actacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg
     6241 gttcggtgct gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg
     6301 tcagtgcttt acatgccgct caacagattg cccaacgcgc aggaggtcaa caatgaacga
     6361 caccagcttc gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg
     6421 ggtgaatccg ggatatccgg ggccaaaaca agccgggccg gatggcgagc gtctgcgttt
     6481 gaaggatggc gcactgtatg acgaggcact gaaatattgc atcaactgca aacgttgcga
     6541 agtcgcctgc ccgtccgatg tgaagattgg cgatatcatc cagcgcgcac gggcgaaata
     6601 tgacaccaca cgcccgtcgc tgcgcaattt tgtgttgagt cataccgacc tgatgggtag
     6661 cgtttccacg ccattcgcac caatcgtcaa taccgctacc tcgctgaaac cagtgcggca
     6721 gctgcttgat gcggcgttaa aaatcgatca tcgccgcacg ctaccgaaat actccttcgg
     6781 tacgttccgt cgctggtatc gcagcgtggc agcccaacaa gcgcaatata aagaccaggt
     6841 ggctttcttt cacggctgct tcgttaacta caaccatccg cagttaggca aagatttaat
     6901 taaagtgctc aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg
     6961 cgtaccgctg atcgccaacg gctttaccga taaagcacgt aaacaggcaa tcacaaatgt
     7021 agagtcgatc cgcgaagcag tgggagtaaa aggcattccg gtgattgcca cctcctcaac
     7081 ctgtacattt gccctgcgcg acgaataccc agaagtgctg aatgtcgaca acaaaggctt
     7141 gcgcgatcac atcgaactgg cgacccgctg gctgtggcgc aagctggacg aaggcaaaac
     7201 gttaccgctg aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa
     7261 aatgggctgg acgctctaca ccctggagct gttgcgtaaa atcccgggac ttgagttaac
     7321 ggtgctggat tcccagtgct gcggtattgc aggtacttac ggtttcaaaa aagagaacta
     7381 ccccacctca caagccatcg gcgcaccact gttccgtcag atagaagaaa gcggcgcaga
     7441 tctggtggtt accgactgcg aaacctgcaa atggcagatt gagatgtcca caagtcttcg
     7501 ctgcgaacat ccgattacgc tgctggccca ggcgctggct taaactcctt tctgatgccc
     7561 ggtaagcatg ttgttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt
     7621 gcaacgcagt gaaaattcct ctgaaaacgt ctcgcaaagg ctaaaactgg cagatgtcaa
     7681 aggcctggga taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac
     7741 cgaatcaaca acctcctccc cgcatgatgc ggtgtttaaa acctttatgt tcacacccga
     7801 aaccgcacgg gattttctgg aaatacatct accagagccg ctgcgcaagc tttgcaacct
     7861 gcaaacctta cgcctggaac ccaccagttt tattgaaaaa agtttacgcg cttactactc
     7921 ggatgttttg tggtccgtgg aaaccagcga cggtgacggt tatatctact gcgtgattga
     7981 acatcaaagc tctgcagaaa agaatatggc ttttcggcta atgcgctatg ccactgccgc
     8041 catgcagcgt cacctggata aaggctatga cagagttccg ctggtggtgc cattgctgtt
     8101 ttatcatggc gaaacctcgc cctacccgta ctcactcaac tggctggatg agtttgacga
     8161 tccgcaactt gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt
     8221 acctgacgat gagattatgc aacatcggcg tatagctctg cttgaactga ttcaaaagca
     8281 tattcgcgac cgcgatttaa tcggtatggt cgacaggatc accacgcttt tggttagagg
     8341 cttcactaat gacagccagc tacaaacgct gtttaattat ctgctgcaat gcggcgatac
     8401 ctcccgtttc acccgtttta ttgaggagat tgccgaacgt tcaccactac aaaaggagag
     8461 attaatgact attgctgaac ggctacggca ggaaggacac caaattggct ggcaggaagg
     8521 tatgcgtgaa caagccatta aaattgcctt gcgcatgctg gaacagggca ttgatcgtga
     8581 cctggtgctc gcggccaccc agctaagcga agccgatctg gcagcgaata accactaatt
     8641 aacacaggcc cacagccgat ccccatgggc ctttgatatc aataactacc ttttatgcgt
     8701 ggcccatttt tgcctgattt aaacatcgcc agtcgttgat ccagggcatc gctgtagagc
     8761 atcgtgtcaa caccaacagc gacaaagttc gctccccacg ccaggcattg ctgcgccata
     8821 tcaggagcca cagccagaaa accagccgct ttacccgcag cacggatccg ccgaatgctg
     8881 gtttcaataa ttcgctgcac ttccgggtgc ccggcgttat ccgggtagcc caacgaggca
     8941 gaaagatccg caggtccaat aaacacgccg tcaatccctt cgacgtcgag gatttcgtcc
     9001 aggttatcca gtgctgtttt actttccacc tgcaccaaca gacaaagcga atcgttgact
     9061 tgcgccatgt aattctcaat gcgtccccag cgcgcagccc gtgccacact ggccccgaca
     9121 ccacgctcac cgtagggagg atagcgcgtg gcagacacca cctgacgtgc ctgatcggca
     9181 gtatcgacca tcgggattag tagagtttgc gcgccaatat ccaggacttg tttaatcagc
     9241 gacttactgc cttccaccgg acggatcacg ggttggctgg catagggcgc taccgcctgt
     9301 agctggtgat aaagatcctg aatggtgttt ggcgcgtgct ccccgtcaat cagcaaccag
     9361 tcataaccgg aggtggcggc aatttctgcc atataagaag tagtcgagct taaccacaga
     9421 ccaatttgca cttcgccttt gcgtaaacgt tctttaaagg gattggttaa taatgcgttc
     9481 atggtgttcc ttaacctatt aatgatgtgc cacgtcggtc tgcgcaacag tgcgatttac
     9541 acgcagagtg aaaataatca gcgaaccgat gaccgccacc gctgccagcg tcagtaatcc
     9601 cgccgcatcg ctggcaaaca acgtttctgc tttcacgcgc aggatcgggg caataaaccc
     9661 gcccactgca ccaaacaggt ttacaaaacc aatccccgcc gccagcgccg taccggaaag
     9721 cagttgtgtc ggcatcgtcc agaacaccgg ctgcacggca ataaacccga tggctgcaac
     9781 acacagcgct acgatcgcca gtactggaga aagcagcccg gagagaccaa taccaatgcc
     9841 cgccgccagt aatgtcagcg ccgcgacatt acgccgttcg ccggttttat cggaatagcg
     9901 cggaataagc caggtcccaa acaaggccgc gacccacgga atggcggtga ccaccgacgc
     9961 cgtaaagccc acttttgtcc ccagcaatgc agcaacctgg gtcggcagga agaaaattaa
    10021 tccgtaaacc gccacctgaa tggtcaggta gataatcgcc agttgccaga ctcggccatt
    10081 acgcagcgca tcgctcaatc gagaagtcac tttctgttgt tcttcacttg ccagttgatt
    10141 gataagcaac gttttttctt gtttactcag aaaacgtgcc tgctccggtg tgtcatcaag
    10201 ccaaaagaat gtgaataccc cagcgccaac tgccagcaat ccttcaatca caaacatcca
    10261 gaatcagccg ggatgcccca taaatccatg catctccagc agcgcgccgg aaagcggtga
    10321 tcccagtgtt aacgccagcg gtgcgcccat atagaacaac cccataatgc tggcgcgatt
    10381 acgctgcgga aaccattgcg aggtgagata aatcatacca gggaagaacc cggcctccgc
    10441 agcaccaagc agagtacgaa cgatcagaaa tttcgcttca gtatcggccc atgccatggc
    10501 tgccgaaaga aatccccaca gcagtgttgt cgtaccaatc caggttctgg cccccatttt
    10561 gcgcatcaaa agattcgccg gaacacccag aaacgcatat accacaaaga aaatgcctgc
    10621 tcccagcgca taagcttcat tactcagccc ggtatcaatc tggtaggttt gtttggcaaa
    10681 accaatattc gaacggtcga gaaatgccag cacatacagc gccaacataa acggaattaa
    10741 acgcgctcgg tttttcttca ccacggcgtc aagcaaagcg gtgctcataa aaagctcctt
    10801 agaatatgtg ggcgttaccg ttgtcacagc aacgcccgga gtgattagtg gctgtagggg
    10861 cgtttcaaat tgcagtcacg attgagttcg acgccgaaac cgggtttatc aagcactgat
    10921 ttatgaatac gaccattcac cggcactggc tcattaagca gaatcggatc aaattgcgga
    10981 cgcatcgttg aacaatccgg gctggtcatc aggaattcgc tgaatggcgt attggtgaag
    11041 gtgatcaccg catggtgaga gtaaacagac gaaccgtgcg gcaccaccag ttgccctctg
    11101 gatttggcga ttgcggcaat ttccaccagc gtggttaagc caccgcacca gccaacatcc
    11161 ggctgcataa tgtcgatacc ggtttctgaa agcgtacgga aagattgcag tgtgccgtga
    11221 tgctcaccgc tggtgaccat cattccgact ggcgcgttgc gtttcagttc gcgataacct
    11281 tcatactgct gtggcggcag gcactcttcg atccatttca ggttataggg cgcgcaagcg
    11341 tgggccagtt tggtggcata gttcacgtcc tgactcatcc agcagtcgag cattaaccag
    11401 aaatcctcac cgcatttttc acgcatatcc gcgaccatag cggcatcttt gtggatcccc
    11461 gcatcgccat catgtggccc ccagtgcgtc ggcattttgc caccgataaa gcccatctct
    11521 tttgccagat ccggacgcgc acctgtggcg tagaactgaa tctcatcacg aactgcgccg
    11581 cctaaaagtt tataaaccgg aagcccgacc actttgccga acagatccca cagagccaga
    11641 tcgacacagg aaatcgtgtt catcaccagg ccaccagagc cggagtaata cagggtggca
    11701 ttgagcattt gatcgtggat cagtttgata tcactgacac atttaccctc aatgaaacgg
    11761 ttaagatgtt tttcgacaat aaaacagccc atttcaccgg ctgtcgaaac ggcgaatccg
    11821 gtctggccgt tttctgcttc gacttcaaca atcaacgtgc caagaacgtt aatgccaaat
    11881 gactggcgtg actgctcgta atcgcggtat ttactcatcg gggtggcaat atgatcatca
    11941 atccagtgat ttgccccctg gtcgtgataa tcaccgccgc cagcgccttt ttctgctgtc
    12001 gcaccgccag taaaccaggc gcgaacctgt ttgatttttg gtagggtcat aatgttctcc
    12061 attgttatga ggcctgtaag tcaaagggac ttttccatcc caacagacgt gaaatatccc
    12121 tggcgcaggc aatggccttg cccgccagat aatcacggta ttcttcattg atttgtaagc
    12181 gggtaccgac caccgaaatc gcagcggtaa gctcgttatt ggcgttaaac accggcgcag
    12241 cgacacaacg gacatcagca taatcttcgc cgttgtcata actccagccc tgacggcgaa
    12301 tacgcgccag ttcctcgtag agccgttgtg gatgggtaat ggtggtgggt gtcatctgct
    12361 cccagaccag ctcttcgata atactttgct gtacagctgc aggttgccag gcaagcaggc
    12421 atttacctat tccggagcga taaagcgaaa ggctttttcc ttcatgggaa cgcacgctga
    12481 tagtggctga tgactccact ttcaaaatgt aataagcgct gccgttatca ataatcccca
    12541 ggtgacataa cagcccggtg gtatccatca gttgcgtcaa acgcggtcga gccagttccc
    12601 ggagatccat cttgcttaac gcatgaccgg aaagctccac cagcttggtc cacaaacaaa
    12661 aattttcctg gtgatcaagg ctaagaaaac gctgacgcct gagttcatta agcagcaaat
    12721 aggccgtgct tttagggatc cccaatgtgt caatgatcgt cgcagcacta caggggccaa
    12781 tgcgggcaat caaattgaga atatcaatag cccgtgttag ggcgggaact ttgcttgatt
    12841 ccaacatact ggactccagt cttagatact ggaatcagtc tcgttgctca gggggagtaa
    12901 aattgtgaag cgcatcaaat cccgcacctc gtccagcgga ctggagaaat tacgcacaaa
    12961 tttgtgaatc agcgcacgga ttttttctta ttgagtacaa caggttggac aaattttgcc
    13021 ggagggggaa tgtgccggtg cagtaaaccg gcacagagtg gcggaatcag acggataagg
    13081 tcatggactc aacaacctca atccagccat gctcactggc aatgtcctgg ccgtttaacc
    13141 aacggcgcag catattcagt gccatcattg cgcacacttc ctgacggata gccaggctgt
    13201 agcgcgtagt gctgaaacgc acacgcagag cgaaagtgcc atctggcgtg gctagcgcaa
    13261 agttgagatg ctcgttctcg aaacccgaaa cagccagtgc cagcccggca aaatggttgg
    13321 cccgccgttc tgtaatccag tgcgcagttt gcgccagggt ttcttcctgt gaaggaacca
    13381 cttcacacgc cagcaatgga gcacctgcgc gagaaagttg caaagccaat aaaccgctgg
    13441 tgaattgctc gctcaacgtc aggctgaact ggcggttttg caattcgcga ctgatctgcg
    13501 cgggcagtcc ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca
    13561 gccacagttt ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga
    13621 taggcattga ggagcgatag cccattgtta cgcccggcgg cagttgtaga gtgtccaggc
    13681 tttgtgccag atcgctttcc gaacgaccaa aggtagttaa acgcagacaa accggcggct
    13741 gtggtaaaga aaaacgctcg cgcagacgcg gcaggatttc gtgctcgacc atcaccttaa
    13801 attctgacgg tacgccgggg gtaaagaaca tcaggcaacg attaagctgc acggcaaaac
    13861 cacaggcggt gcctaccggg ttattgataa attcagcact ggcaggtagc tccgcttgtt
    13921 tacggttgct cggtgccatt actcgtccac gttcgtggaa atagcgttcc atctctttga
    13981 gccaggcttc atgcaacacc aggccttcac ctcttgctgt cgcagcggcg agtgcgctta
    14041 aatcatcact ggtcggcccc agcccgccgt taacgatcag cacatcggcg tgctgactcc
    14101 gttcgcgaag aatggtcact aagtcatcaa ggttatcccc caccgtattg cggcgagata
    14161 atggcaaccc ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt
    14221 gcccgtgtaa cacttcatcc ccggtggata gcatttccac ttttaacatg gttatctcct
    14281 gcttttgtta tcgaatcact ataacgcaaa gggagataag gcgtagtaag aaactgaatg
    14341 gcgcggcaga atgccgcgcc ggaagggatc agaaactggc gttaacccca acatacgggc
    14401 cgtcagccac ggcgttgtcg cggttaccat ctttacccga cagattcagg tagcgataac
    14461 ccgcctcaat gctgactgga cgcataatgg tgtaacgcgc gccagcattc gcttcctcat
    14521 aactttgaat accgctggag agcgaatccg gagagtagta atactcacca aacaagcgga
    14581 agctgtcacc aatttgccac tgcaaaccac cgccaactgc cgctgcataa ccttcatcac
    14641 cgtaattcgg gttggtgtaa acgccttttc cgccaacggt cgccattaat ggcccgagag
    14701 gcagattcaa ccccagcccc acgcccgcgg catcaccgtc gtcgtcgtta tgtgtccagt
    14761 taccgcttaa cgccaggccc gtcgattcag tcccaaaacc gacaccaata ttggtgtatt
    14821 ctttacccgc ctggccgctg atgctcattg cattgaccga tgcagaaacc aacagcattc
    14881 cggccagacc tgctagcgca atttttttca ttatcctctt tcccacaagc aattaatcaa
    14941 aattcccaa
//
LOCUS       DARNVM010000116        14912 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.116, whole genome shotgun sequence.
ACCESSION   DARNVM010000116 DARNVM010000000
VERSION     DARNVM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.116"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(20..2299)
                     /gene="maeB"
                     /locus_tag="SI621_003285"
     CDS             complement(20..2299)
                     /gene="maeB"
                     /locus_tag="SI621_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HEI4065957.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERVPELMIDGEMHGDAALVEAIRSDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2588..3538
                     /gene="tal"
                     /locus_tag="SI621_003286"
     CDS             2588..3538
                     /gene="tal"
                     /locus_tag="SI621_003286"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEI4065958.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3558..5561
                     /gene="tkt"
                     /locus_tag="SI621_003287"
     CDS             3558..5561
                     /gene="tkt"
                     /locus_tag="SI621_003287"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEI4065959.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLTAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEQAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5664..6707)
                     /locus_tag="SI621_003288"
     CDS             complement(5664..6707)
                     /locus_tag="SI621_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEI4065960.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6833..7408)
                     /gene="nudK"
                     /locus_tag="SI621_003289"
     CDS             complement(6833..7408)
                     /gene="nudK"
                     /locus_tag="SI621_003289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HEI4065961.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7476..9455)
                     /gene="aegA"
                     /locus_tag="SI621_003290"
     CDS             complement(7476..9455)
                     /gene="aegA"
                     /locus_tag="SI621_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HEI4065962.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9661..11361
                     /gene="narQ"
                     /locus_tag="SI621_003291"
     CDS             9661..11361
                     /gene="narQ"
                     /locus_tag="SI621_003291"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HEI4065963.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11525..14638
                     /gene="acrD"
                     /locus_tag="SI621_003292"
     CDS             11525..14638
                     /gene="acrD"
                     /locus_tag="SI621_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HEI4065964.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14737..14796)
                     /gene="ypfM"
                     /locus_tag="SI621_003293"
     CDS             complement(14737..14796)
                     /gene="ypfM"
                     /locus_tag="SI621_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HEI4065965.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3467 a         3972 c         3921 g         3552 t
ORIGIN      
        1 gcgtgagagt taaaaaaaat tacagcggtt gggtttgtgc ttcaaccacg gctagcgcca
       61 ccatgttgac gatacgacgt accgatgcga tcggcgttaa cacgtgaacc ggtttcgcca
      121 cgcccatcag caccgggccg acagtcacac cttccgagct ggaaacgcgc aataagttgt
      181 aactaatgcg ggcagcttcc atgttcggca tcaccagaat attagcggaa cctttcaaag
      241 ggctgtccgg catacggtcg ctgcgaatcg cttccaccag cgctgcatcg ccgtgcattt
      301 caccatcaat catcagttct ggtacacgtt ccctgaccag ttccagcgcc tgacgcattt
      361 tgctcgacga cgggcagtca gaagaaccaa agttggagtg cgacaacaga gccacgcgcg
      421 gctcaatacc aaaacgacga acagtttctg ccgccatcaa ggtgatctcc gccagctctt
      481 ctgcgtctgg ttcgtcattg acgtatgtat cggcaataaa ggtgttacca ctcggcagca
      541 gcagcgcgtt catggcacct gcggtgtgaa cgccatcgcg ataaccaaag acatttttca
      601 ccacgctaaa atgttcatga taatcgccca ccgtaccgca gatcattgca tcggcttcgc
      661 cacgctgaac catgatcgcg ccaatcactg tcgggttact gatcaatgca cgctgcgcct
      721 gttcctgagt gacaccgcga cgcttcatga tctggaagta ttcggtccag tactctttaa
      781 agcgcggatc ggattcgtta ttgacgatct caaaatcaac acccgctttg atctgcaagc
      841 ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc gataaggatc ggtttcgcca
      901 gccccagcgt taccagttcc tgagtggcat gcagaacgcg cgcctcttcc ccttccggca
      961 gaacgacgcg cttcggcgct ttgcgagcct gggagaaaat cggcttcata aacaggttgg
     1021 ttttgtaaac gaactccgtc agcttgtcga tgtagacgtc gaaatcagca atcggacgcg
     1081 tcgccacgcc cgactccatc gccgctttag cgaccgcagg agcgatctta acgatcaagc
     1141 gcggatcaaa tggttttgga atgatgtatt ccggaccaaa gctcagatcc tgatcgccat
     1201 acgctgaagc cactacttca ctctgttccg catgggccag ttctgcaatc gcgcgtaccg
     1261 ccgccagttt catctcttcg ttgatggcgg ttgcgccaac gtccagcgcg ccacggaaga
     1321 tgaacgggaa gcaaagaacg ttgttcacct ggttcgggta gtcggaacgg ccggtacaga
     1381 tgatggcatc cggacgcact tctttcgcca gcggcggcag aatttccggt tccgggttcg
     1441 ccagcgccag gatcatcggt gcccgagcca ttttcttcac catttcctgg gtcagcactt
     1501 tcgggccgga acagccgagg aaaatatccg cgccttcaat cacatcatcg agggtacgtt
     1561 tgccgtcatc caccaccgca tatgcggctt tggtttccgc catgtttggc tcacggccct
     1621 gatagataac gccttttgaa tcgcaaacca cgatgttatg tttttgcagc cccagagcca
     1681 ccagcaggtt catacaggcg attgctgcgg cacccgcgcc ggaaaccacc attcgcacgt
     1741 cagagatgtt tttctccacc acgcgcaaac cgttgaggat ggcggcagtg ctgatgattg
     1801 ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc agtttctgtt
     1861 caatatagaa acattctggc gctttaatgt cttcgaggtt gatgccgccg aaggtcggtt
     1921 caagcgcggc gacgacttca ataaatttgt ccgggtcgag ttcatcaact tcaatgtcaa
     1981 atacatcaat cccggcgaat ttcttaaaca gaacgccctt gccttccatc accggtttgc
     2041 ccgccagcgc gccaatgttg cctaacccca gcaccgccgt accgttagag atcaccgcca
     2101 ccaggttacc gcgcgcggta tatttgtagg cttttaacgg gtctttttcg atttcaagac
     2161 aaggtgcggc aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt gccagaggct
     2221 tggttggaga aacctggatt ttccctggaa ctggaaattc atggaaatca agtgcacttt
     2281 gttttaactg gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa taaagtgtgt
     2341 gggcaacagc ccctgcccac aacgtggcgc acattattac cttgccggag tctacagact
     2401 ttgagcaagt ccaaactctc accattaata taatgttttg gtaataatcc tataacactg
     2461 atgttacctg cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta
     2521 gcgaaatttc tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt
     2581 atttcctatg aacgagttag acggcatcaa acagttcact actgtcgtgg cagacagcgg
     2641 cgatattgag tccattcgcc attatcatcc ccaggatgcc accaccaatc cttcgctgtt
     2701 actcaaggct gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg
     2761 taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca aactggcggt
     2821 caatttcggt gctgaaattc tcaaaatcgt acccggtcga gtgtcaacag aagttgatgc
     2881 acgcctctct tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta
     2941 tcagcaacaa ggcgttgaga aatcacgcat tctgatcaaa ctggcttcga cctgggaagg
     3001 aattcgcgcg gcagaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt
     3061 ttcttttgcc caggcgcggg cctgtgcaga agcaggcgta tttctgattt cgccgtttgt
     3121 cgggcgtatt tatgactggt atcaggcacg caagccgatg gacccgtatg tggttgaaga
     3181 agatccgggc gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga
     3241 aaccattgtg atgggcgcga gcttccgtcg caccgaacaa atccttgcct taaccggctg
     3301 cgatcgactg actatcgcac cgaatttgct gaaggagctg caggaaaaag tttcgccagt
     3361 ggtacgtaaa ttaatcccac cttctcagac gttcccacgc ccagctccca tgagcgaagc
     3421 ggagttccgt tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat
     3481 tcgtctgttc gccgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac
     3541 cagccacgga gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta
     3601 tggatgcggt acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata
     3661 ttgccgaagt gctgtggaac gattttctta aacataaccc caccgaccca acctggtatg
     3721 atcgcgaccg ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctgc
     3781 atctgaccgg ttacgacctg ccgctggaag agctgaagaa cttccgtcag ttgcattcaa
     3841 aaaccccagg tcacccggag attggttata cgccaggtgt tgaaaccacc accggtccac
     3901 ttggacaagg tttggcgaac gccgtcgggc tggcaatagc agagcgtaca ctgacggcgc
     3961 agtttaatca gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg
     4021 gctgtctgat ggaaggtatc tcccatgaag tttgttcgct ggcgggcacg ctggggctgg
     4081 gcaaactgat tggtttttac gatcacaacg gtatttccat cgacggtgaa accgaaggct
     4141 ggtttaccga cgatacagca aaacgttttg aagcctatca ctggcatgtg atccacgaga
     4201 tcgacggcca cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag
     4261 ataagccgtc gctgattatc tgtcgtacag tgattggctt tggatcgccg aataaagcag
     4321 gtaaggaaga ggcgcatggc gcaccgctgg gggaagaaga agtggcgctg gcacggcaaa
     4381 aactgggctg gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg
     4441 cccgcgaaaa aggcgaacaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa
     4501 aggctcatcc gcaactggca gaagagttta cccgtcggat gagcggcggt ttaccgaagg
     4561 actgggagaa aacgactcag aaatatatca atgagttgca ggcgaatccg gcgaaaatcg
     4621 ccacccgtaa ggcttcgcaa aatacgctta acgcttatgg ccccttgcta ccggagctgc
     4681 tcggcggttc ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtgtcgc
     4741 tgaaggaaga tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg
     4801 ctatcgccaa cggcattgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga
     4861 tgtttgttga atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga
     4921 ttatggttta tacccacgac tcaattggct taggggaaga tggtccgacc caccaggccg
     4981 ttgagcaact ggccagtctg cgcttgacgc cgaatttcag cacctggcga ccgtgcgatc
     5041 aggtggaagc ggcggtaggc tggaagcttg cggttgagcg ccacaacgga ccgacggcac
     5101 tgatcctctc aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga
     5161 ttgctcgtgg tggttatgtg ttgaaagaca gcggcggcaa gccggatatt attctgattg
     5221 ccaccggttc agagatggaa atcaccctgc aagcggcaga gaaattagca ggagaaggtc
     5281 gcaatgtacg cgtagtttcg ctgccctcga ccgatatttt cgacgcccag gatgaggaat
     5341 atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggcatcg
     5401 ccgattactg gtacaaatat gttggtctga aaggggcaat tgtcgggatg accggctatg
     5461 gggaatctgc tccggcagat aagctgttcc cgttctttgg ctttaccgcc gagaatattg
     5521 tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgcta
     5581 tatgttcccc tcaccctaac cctctcccca gaggggcgag gggacggtcc gtgcgctttt
     5641 taggggcgca tctgggattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc
     5701 ccatgccagt tatcgcaggt gggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg
     5761 tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc
     5821 accagttcac gcgatttttc atcgaatgtt gcgttcatca gttccagttc attcgtctcc
     5881 tgaccgctgt tgaacggcaa acgtaaccga accgggcgcg aggccagtgg ctttttacgc
     5941 gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc
     6001 agcgctttat catcggtcag cgcagtgaca ttcacctctc gacgcaatgg atcaagcgag
     6061 cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt
     6121 gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt
     6181 acgctgagcg gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt
     6241 tgctgagcat caataaacaa caacgccgct tttaagccgc tcagagagat ggtctgattg
     6301 ccgtcgcgta aggtgatcgc cttcccttcc tgaatcatct gcaaaaacgc ggtgatggtt
     6361 gccgtatcat cggttactag taaccatggt gaaatccgcc acttgtcacc acttaacgcc
     6421 aacggctcac catctaacag cagccgtggc gctatctccc cttcagacgc atccggcgac
     6481 ttcaacccgc cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc
     6541 agggtcatca ccagtccgtt atgatcgccc gtattacgcg ccacgcagaa gttttggtta
     6601 ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac
     6661 gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc
     6721 ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat
     6781 cggatttatc ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca
     6841 ttaaatgtga catttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc
     6901 cggttttgat catctccagc gcctggctga acggcagctc gagcacttca atatcttcat
     6961 cttcgacacc gccaccggcg ttagcgcgct ggctgtcact gtattcggcg ataaagaagt
     7021 ggattagctc agtcacaccg cctggcgaca tatacagttc aaataatttg cgcacttcgc
     7081 caacttcata acccgtctct tcaatcgctt ctttacgaat acacacttcc ggttcgtcgt
     7141 tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg
     7201 tagcgacacg gaactgacga atcagaacca cagtcttttt cttcgcgttg tacaggagga
     7261 tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt
     7321 tgcgcgtgag atcgtaggta atgttgtgca gggtgaaata gttatcggag agaattttgt
     7381 ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc
     7441 ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc
     7501 cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc
     7561 agatccgcac cacgcacggc gtcaccaccg gcgaagattt tcggattcgt ggtctggtaa
     7621 cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc
     7681 gactccagcc acggcatccc gtgtggattg aagccaaacg ccataatcac cgcgtcggct
     7741 ggcatgacaa attcactgcc ttccaccggt actggacgcc gacgcccctg ggcatccggc
     7801 tcgccaagac gcgtacgcaa gaaacgaatc ccgcaaacgt gaccttgctc attcagctca
     7861 agcgccaccg gctggacgtt aaattcaaag ttggccccct cttcgcgggc gttcttcacc
     7921 tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc
     7981 gcgccgtggc gcagcgcggt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc
     8041 acgacgttga gtccggcggt attgataaac ggctcttccg gtagctcttc gagtcccatc
     8101 acctgtttag tgttggcgat gaggaacggc agcgcgtcat aaacgcctgg cgcatcttca
     8161 ttgggtaaac ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat
     8221 tgttccaaaa gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga
     8281 atccccatcg cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa
     8341 gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc
     8401 gccacgccat tgcgggtcag aacatccgca caggccagcc ctgctggacc cgcaccgata
     8461 atcgccaccc gcttgtccac tttggtgaca tggcttaagt caggacgcca acctttcgcc
     8521 aacgcctgat ctgaaatgta gcgttcaatg ttgccgatag ttactgcacc gtgctcatcg
     8581 cgaatagtac aggctccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc
     8641 ggcagggtgt tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc
     8701 agttcaatcc actgtggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc
     8761 tcgccgcact taaggcagcg cgaggcttcc cgttgtgcct gatcggcacg gaatggcaga
     8821 taaatttcat caaaaccggt ttttcgcgct tcaatcgcca gtttgtccgg ctcgccacgc
     8881 gcgggcgttg cctgcatttg ctcgacttta ctcattaccg gcatttcttg tgccgcggta
     8941 ctggcatgcc acggttgatg ttcctgacgt gcggtgcgca ggcggcggga tttcgccatg
     9001 ccggagagtg cggcatcagt gaccagttgc agcgcgtctg ccgggcaatt ctcgacacag
     9061 gcaggaccgt tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttg
     9121 cccgccgcga cgggtgtcag gacgatttgc atcgtaccaa atggacaggc caccacgcag
     9181 gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt
     9241 gcgccattag ggcaactacg ggcgcagggc gcatcttcgc aatggtgaca ggtcactgca
     9301 ctacgttgct gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg
     9361 acatgttgct catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga
     9421 cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa
     9481 aaccttattc tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc
     9541 atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca
     9601 ctgactaaat tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt
     9661 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtcctg
     9721 ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct
     9781 gaggctatca atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgatttg
     9841 caaagtggca gtccacaact caatgcacat cgccagttat ttcagcaggc actgcattca
     9901 ccggtattaa ccaatctcaa cgtctggtat gtgccagaag cagtaaaaac ccgctatgcg
     9961 catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggtga tttgccgtgg
    10021 tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtact ggctttacag
    10081 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggcatcggt
    10141 attttcacgc tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg
    10201 aatcagctgg ttaccgccag tcagcgtatt gaacacggac agttcgactc gccaccgctg
    10261 gataccagcc tgccgaatga gcttggcctg cttgcaaaaa cctttaacca gatgtcgagc
    10321 gagctacata aattgtaccg ttcgctggag gcgtcagtag aagaaaagac ccgcgacctc
    10381 cacgaggcca agcgtcgtct ggaggtgttg tatcagtgtt cgcaggcgct gaacaccagc
    10441 cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct
    10501 gaatatctgg agttaaatgt cggtgaaaac tggcggatta gcgaagggaa accaaacccg
    10561 gaattgccga tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac
    10621 tggcaaaata gtcacgtttc atcatcagaa ccgctgctta acagtgtttc gtcgatgctg
    10681 ggacgcggtt tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa
    10741 gaacgtgcga ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta
    10801 cgtatccagt tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt
    10861 atcatggccg atttttccca agcgttgaat gatgcttatc ggcagttacg cgagctgttg
    10921 actactttcc gcctgacgct gcagcaggca gatctcccct ccgcgttgag ggaaatgctg
    10981 gatacgttac aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg
    11041 gcgctggatg cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat
    11101 gcgatgaagc acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc
    11161 aatcacacgg tctatatccg tgataacgga attggcatcg gtgaaccgaa agaacccgaa
    11221 ggtcattatg gcctgaatat catgcgcgag cgcgcggaac ggctgggtgg gacgctgact
    11281 ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc cgcggagggt
    11341 gaggaaagtc agctgatgta atgcctccga ctgaccaaag aatactggcc cttaaagttc
    11401 agtataaaag ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac
    11461 cttgccgcta cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct
    11521 tttaatggcg aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt
    11581 gtgtctgaca gggaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc
    11641 gccaccgaat gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa
    11701 caccgtgacc caggttatcg agcaaaatat gaccggcctc gataatttga tgtatatgtc
    11761 gtctcagagc agtggcaccg gccaggcatc tgtcacttta agttttaaag caggcaccga
    11821 tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc
    11881 gcaggcggtg caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac
    11941 cattgccttc gtctctaccg atggttcgat ggataaacag gatattgccg attacgttgc
    12001 cagtaatatt caggatccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg
    12061 ttcgcaatat tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac
    12121 ggctaaagat gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact
    12181 tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact
    12241 gctgcaaaca ccagaacagt tccgcgatat caccttgcgg gtgaatcagg acggctcaga
    12301 ggtaaggctg ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag
    12361 ccgcttcaat ggtaagccag cctccgggtt gggggtaaaa ctggcctccg gcgcgaacga
    12421 aatggcgaca gcggagctgg tgctcaatcg tcttgacgag ctggcgcagt atttcccgca
    12481 tggactggaa tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga
    12541 cgtggtgaaa acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct
    12601 gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac
    12661 cttctccgta ctttatgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt
    12721 gctggcgatc ggtctgctgg tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat
    12781 tatgagtgag gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca
    12841 gggggcactg gtcgggattg cgatggtgct ttcggcggta tttgtaccaa tggccttctt
    12901 cggcggcacc accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt
    12961 gctgtcagta ctggtagcga tgatcctcac tccggctctg tgtgccacgc tacttaagcc
    13021 actgaaaaag ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt
    13081 taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg
    13141 ctggattgtg atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc
    13201 gacgtcgttc ttgccacttg aagaccgtgg catgtttact acctcggtac agttgcccag
    13261 cggttcaacc caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac
    13321 ccatgaaaaa gacaacatca tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa
    13381 cgggcaaaac gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa
    13441 gaccggcacc tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaaca agattaaaga
    13501 agctcgcgtt atcgccagca gcccgccagc aattagcggt cttggcagtt ctgcaggttt
    13561 tgatatggag ttgcaggacc acgctggggc aggtcacgat gcgctgatgg cagcacgtaa
    13621 ccagttgctg gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct
    13681 cgatgacagt ccgcagttgc agatcgatat cgaccagcgt aaagctcagg cgctgggcgt
    13741 tgctatcgac gatattaacg acacgctgca aaccgcctgg ggttcgagct atgtgaatga
    13801 ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc catatcgcat
    13861 gctgccagat gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt
    13921 ctctgctttc gcgacctcac gctgggagac aggctcgccg cgtctggaac gctataacgg
    13981 ttattctgcg gtagagattg tcggggaagc cgcaccgggg gtcagtaccg gtacggcgat
    14041 ggatattatg gaatcgttag tgaagcagct gccaaacggc tttggtctgg agtggacggc
    14101 gatgtcgtat caggagcggc tttccggcgc gcaggctccg gcgctgtacg ccatttcctt
    14161 gctggtggta ttcctgtgtc tggcggcgct gtatgaaagc tggtcggtgc cgttctcggt
    14221 aatgctggtc gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct
    14281 ggaaaacgac gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa
    14341 cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga
    14401 agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt
    14461 tatcttcggc gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc
    14521 ggtgggtact ggcgtaatgg gcgggatgat ttcggccact attctggcta tttacttcgt
    14581 gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc
    14641 aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct
    14701 cagcgggttc ttgctgtttg tactttgtct caggaattac ttacgaagca taacttcgat
    14761 aaagtctttc cagttcccca gttcacgttc aatcataaca acctctctta taattatggg
    14821 tattctacgg aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac
    14881 ctccggggga caaaccttat gtaaatacta tg
//
LOCUS       DARNVM010000117        14821 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.117, whole genome shotgun sequence.
ACCESSION   DARNVM010000117 DARNVM010000000
VERSION     DARNVM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.117"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(227..1258)
                     /gene="rsmC"
                     /locus_tag="SI621_003294"
     CDS             complement(227..1258)
                     /gene="rsmC"
                     /locus_tag="SI621_003294"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HEI4065966.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPEFDADKFWG
                     EYNVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1361..1774
                     /gene="holD"
                     /locus_tag="SI621_003295"
     CDS             1361..1774
                     /gene="holD"
                     /locus_tag="SI621_003295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HEI4065967.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRLGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKISMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPMARAALWQQICTYEHDFFPRND"
     gene            1743..2189
                     /gene="rimI"
                     /locus_tag="SI621_003296"
     CDS             1743..2189
                     /gene="rimI"
                     /locus_tag="SI621_003296"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEI4065968.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2204..2881
                     /gene="yjjG"
                     /locus_tag="SI621_003297"
     CDS             2204..2881
                     /gene="yjjG"
                     /locus_tag="SI621_003297"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEI4065969.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2972..4561
                     /gene="prfC"
                     /locus_tag="SI621_003298"
     CDS             2972..4561
                     /gene="prfC"
                     /locus_tag="SI621_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEI4065970.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4954..5559
                     /gene="osmY"
                     /locus_tag="SI621_003299"
     CDS             4954..5559
                     /gene="osmY"
                     /locus_tag="SI621_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HEI4065971.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5686..5847
                     /locus_tag="SI621_003300"
     CDS             5686..5847
                     /locus_tag="SI621_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEI4065972.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5969..7042
                     /locus_tag="SI621_003301"
     CDS             5969..7042
                     /locus_tag="SI621_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEI4065973.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYCDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7039..7818
                     /locus_tag="SI621_003302"
     CDS             7039..7818
                     /locus_tag="SI621_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEI4065974.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(7852..8715)
                     /locus_tag="SI621_003303"
     CDS             complement(7852..8715)
                     /locus_tag="SI621_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HEI4065975.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKVLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8687..10237)
                     /locus_tag="SI621_003304"
     CDS             complement(8687..10237)
                     /locus_tag="SI621_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HEI4065976.1"
                     /translation="MPTSHEIALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLQEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFARTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10495..11274
                     /gene="deoC"
                     /locus_tag="SI621_003305"
     CDS             10495..11274
                     /gene="deoC"
                     /locus_tag="SI621_003305"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEI4065977.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKDACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11352..12674
                     /gene="deoA"
                     /locus_tag="SI621_003306"
     CDS             11352..12674
                     /gene="deoA"
                     /locus_tag="SI621_003306"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEI4065978.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLTSGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12726..13949
                     /gene="deoB"
                     /locus_tag="SI621_003307"
     CDS             12726..13949
                     /gene="deoB"
                     /locus_tag="SI621_003307"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEI4065979.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14006..14725
                     /gene="deoD"
                     /locus_tag="SI621_003308"
     CDS             14006..14725
                     /gene="deoD"
                     /locus_tag="SI621_003308"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEI4065980.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3525 a         3881 c         4000 g         3415 t
ORIGIN      
        1 cgcgtctacc aattccgcca ccttcgcata ccatcaattc ttaaaaagaa ttgctaccac
       61 ggaggcgcat tctagtggtt ttcagctttt cgtcaatagt taattatcgc cggaggtgta
      121 attgctggaa aaatgtccat cagcgaattg tggataattc gtttgtcggt cagagaaggc
      181 gtgtacgccg catccgacac ggtatgcggc gacagatatc agatactcaa cctttcttcg
      241 cctggcgggt cataatggcg cgatacacct tgaagcgccc ggtttgcgcg atcacttcgt
      301 ggaagccaaa tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc gctacaattc
      361 gcagctcgcc gccgctatta agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat
      421 ccaggctggt ttgcatccca tcgtggaacg gcgggttgga gatgatcata tcaaaacgac
      481 ctttcacctc ggaaaagacg ttactggcaa agacttcacc ttcaacaccg ttggccgcaa
      541 gtgttgcgcg gctggcttct accgctggcg cagagacatc gcacagagtg agacgaattt
      601 tcggcgaatg gcgcgcaaag gcaacagaaa gcacgcccgc gccacagccg acatccagca
      661 ctttaccttt cgtgtgcgga gttaacgtcg agagcagtaa ctggctaccg acatccagac
      721 cgtcgcggct aaacacgccc ggcagcgttt tgaccgtcag gccatccacg ttatattcgc
      781 cccagaattt atccgcatca aattccggct gtttttccag acgaccaaaa tagaggccgc
      841 agcgacgggc gctgtcgact ttattcaacg gcgcataatc tgccagcatc tgttcggcgc
      901 tgcgcacgcc gctgcggttc tcgccaacga caaaaatatc cgtgcccacc ggcagcagag
      961 aaagtaaatt catcaactgg aactgggctt ccggtttatt cttcggccag tagtagatca
     1021 gcgtatcgca atctgcgacg tcatctgccg ttgcgaccag actaaaacgg gcgttatccc
     1081 ccatctggcg gcttaacacc tgccaatggt ggaattgctg ggtatgagca cggctggccg
     1141 cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga atacggcttt
     1201 gttcgaaatc atcactgtga cgcagcaaga cttcacttgc cggggtaaat gcagacatgg
     1261 aatgctcctc aattgatact ggcggcgatt atagccatat gttggcgcgg tatcgacgaa
     1321 tttgctatat ttgcgcccct gacaacagga gcgattcgct atgacatccc gacgagactg
     1381 gcagttacag caactgggca ttacccagtg gtcgctgcgt cgccttggcg cgttgcaggg
     1441 ggagattgcc attgcgatcc cggcacacgt ccgtctggtg atggtggcaa acgatcttcc
     1501 cgccctgact gatcctttag tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca
     1561 agtgctgcaa ctgacgccag aaaaaatctc gatgctgcca caaggcagtc gctgcaacag
     1621 ctggcggttg ggtactgacg aaccgctatc actggaaggc gctcaggtgg catcaccggc
     1681 gctcaccgaa ttacgggcaa acccaatggc acgcgccgcg ttatggcaac aaatttgcac
     1741 atatgaacac gatttcttcc ctcgaaacga ctgatttacc agcggcttac cacattgaac
     1801 aacgcgccca cgcctttccg tggagtgaga aaacctttgc cagcaaccag ggcgagcgtt
     1861 atctcaactt tcagttaacg caaaacggca aaatggcggc gtttgcgatt acgcaagtgg
     1921 tgctggatga agctacattg ttcaatattg cggtcgatcc tgactatcag cgtcagggat
     1981 tgggaagggc gctgctggaa catctgatcg acgaactgga aaaacgcggc gtggcgacgc
     2041 tatggctgga agtccgtgcc tcaaacgctg ccgccattgc actgtacgaa agtttaggct
     2101 ttaacgaggc gacgattcgc cgcaattact accccaccac ggacggtcgc gaagacgcca
     2161 tcatcatggc gttgccaatc agtatgtaag acaaggtgga ataatgaagt gggactggat
     2221 tttctttgat gccgatgaaa cgctgtttac ctttgactca ttcaccggcc tgcagcggat
     2281 gtttcttgat tacagcgtca cctttaccgc tgaagatttt caggactatc aggccgttaa
     2341 caagccactg tgggtggatt atcaaaacgg cgcgatcact tcattacagc ttcagcacgg
     2401 gcggtttgag agctgggccg aacggctgaa cgtcgagccg ggtaaactca acgaagcctt
     2461 tattaatgcg atggcggaaa tctgtacgcc gctgccgggc gcggtttctc tgcttaacgc
     2521 cattcgtggc aacgctaaaa ttggcatcat caccaacggc tttagcgcct tgcagcaagt
     2581 gcgtctggaa cgcacgggcc tgcgtgatta tttcgatttg ctggtgattt ccgaagaagt
     2641 tggcgttgcc aaaccgaata agaaaatttt cgattatgcg ctggaacagg cgggcaatcc
     2701 tgaccgttca cgcgtgctga tggttggcga cactgccgag tccgatattc tcggtggcat
     2761 caacgccggg cttgcgacct gctggctgaa tgcgcacaat cgcgagcaac cagaaggcat
     2821 cgcgcctacc tggaccgttt cttcgttgca cgaactggag cagctcctgt gtaaacactg
     2881 attgcctccc ccccgttgat gggtaaaata gccgcaattt ttcgttttca acaagcgcgg
     2941 cgcgatgccg cttactcaag aagaaagaat tatgacgttg tctccttatt tgcaagaggt
     3001 ggcgaagcgc cgcacttttg ccattatttc tcacccagac gccggtaaga ctaccatcac
     3061 cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc ggtacagtaa aaggccgtgg
     3121 ttccaaccag cacgctaagt cggactggat ggagatggaa aagcagcgtg ggatctccat
     3181 tactacgtct gtgatgcagt ttccgtatca cgattgcctg gttaacctgc tcgacacccc
     3241 ggggcacgaa gacttctcgg aagataccta tcgtaccctg acggcggtgg actgctgcct
     3301 gatggttatc gacgccgcaa aaggtgttga agatcgtacc cgcaagctga tggaagttac
     3361 ccgtctgcgc gatacgccga tcctcacctt tatgaacaaa cttgaccgtg atatccgcga
     3421 cccgatggaa ctgctcgatg aagttgagaa cgagctgaaa atcggctgcg caccgatcac
     3481 ctggccgatt ggctgcggca agctgtttaa aggcgtttac cacctttata aagatgaaac
     3541 ctatctttat cagagcggta aaggccacac cattcaggaa gtccgtattg ttaaagggct
     3601 gaataacccg gatctcgacg ctgcggttgg tgaagatctg gcgcagcagc tgcgtgatga
     3661 actggagctg gtgaaaggcg cgtctaacga gttcgacaaa gaattgttcc ttgcgggcga
     3721 aatcactccg gtattctttg gtactgcgct gggtaacttc ggcgtcgatc atatgctgga
     3781 tggtctggtg gagtgggcac ctgcgccgat gccgcgtcag actgataccc gtaccgtaga
     3841 agcgagcgaa gacaaattta ccggcttcgt atttaaaatt caggccaaca tggacccgaa
     3901 acaccgcgac cgcgtggcgt ttatgcgcgt ggtatccggt aaatatgaaa aaggcatgaa
     3961 actgcgccag gtgcgcaccg cgaaagatgt ggtgatctcc gacgcgctga cctttatggc
     4021 gggcgaccgt tcgcacgttg aagaagcgta tccgggagac atcctcggcc tgcacaacca
     4081 cggcaccatt cagatcggcg acacctttac ccagggtgag atgatgaagt tcaccggtat
     4141 tccgaacttc gcaccagaac tgttccgtcg tatccgcctg aaagatccac tgaagcaaaa
     4201 acagctgctc aaagggctgg tacagctttc cgaagagggc gcagtgcagg tgttccgtcc
     4261 gatctccaac aacgacctga tcgttggtgc agttggtgtg ctgcagtttg atgtggtggt
     4321 agcgcgcctg aagagcgaat acaacgttga agcagtgtat gagtcagtca acgttgccac
     4381 tgcacgctgg gtagaatgtg cggacgcgaa gaaattcgaa gagttcaagc gtaagaacga
     4441 aagccaactg gcgcttgatg gcggcgataa cctcgcttac atcgctacca gcatggtcaa
     4501 cctgcgtctg gcgcaggaac gttatccgga cgttcagttc caccagaccc gcgagcatta
     4561 attcttgcct ccagggcgcg gtagccgctg cgccctgtca atttcccttc cttattagcc
     4621 gcttacggaa tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg
     4681 gtttcaaaat tgtgatctat atttaacaaa gtaatgacat ttctgacggc gttaaatacc
     4741 gttcaatgcg tagatatcag tatctaaagc cgtcgattgt cattctaccg atattaataa
     4801 ctgattcaga ggctgtaatg gtcgttattc atcacccatc gcttttgtga tggcgaccat
     4861 tgacttctgt agagggtgaa gtctctccct attcagcaat gcaacctcgt gttgccaggc
     4921 tcaaattacg agcaaacata caggaataaa tcgatgacta tgacaagact gaagatttcg
     4981 aaaactctgc tggctgtaat gttgacctct gccgtcgcga ccggctctgc ctacgcggaa
     5041 aacaacgcgc agactaccaa tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa
     5101 gtcggtaatt tcatggatga cagcgccatc accgcgaaag tgaaggcggc gctggtggat
     5161 catgacaaca ttaagagcac cgatatctct gtcaaaaccg atcaaaaagt cgtgaccctg
     5221 agcggtttcg ttgaaagcca ggctcaggcc gaagaggcag tgaaagtggc gaaaggcgtt
     5281 gaaggggtga cctctgtcag cgacaaactg cacgttcgcg acgctaaaga aggctcggtg
     5341 aagggctacg cgggtgacac cgccaccacc agtgaaatca aagccaaact gctggcggac
     5401 gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg acggcgtggt tcagctctcc
     5461 ggtaccgtcg attcccaggc acagagcgac cgtgctgaaa gtatcgccaa agcggtagat
     5521 ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc
     5581 gaactttgtc gggaggcgcg atgtgcacca cactaaaaat atcgcattga gtgattttca
     5641 cgctcatatc aagcggtgac attaactatg gtaaaggaga cgcttatgtt tcgttggggc
     5701 atcatatttc tggttatcgc gttaatcgcc gccgcacttg ggtttggtgg tctggccggt
     5761 accgctgcag gcgcagctaa aattgtcttt gtcgtcggga ttattctgtt cctggtgagt
     5821 ttgttcatgg gccgaaaacg accctagatt tcagaccatt aagaaaattt tatccaaagc
     5881 cagtccagcg gactggcttt tgcggtttta gcgaataata aattgcgtta ctttgtcatt
     5941 ctttcacaac aaaaacagga aggcagaggt ggggcagcga atacctgtaa cgcttggtaa
     6001 tattgcgccg ttgtcgctaa ggccgttcca gcctggacga atagctctgg tgtgcgaagg
     6061 cggcggacag cgtggaattt tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt
     6121 taatcctttc gatctttatc tcggcacatc tgccggggcg cagaacctct cggcgtatat
     6181 ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc tataccacaa aacgcgaatt
     6241 tttcgatcca ttgcgctttg tccgtggagg aaatcttatc gatctcgact ggctggtgga
     6301 ggccactgca agccagatgc cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg
     6361 caaatcgttt tatatgtgcg cctgtcgtca ggatgactat gcgccgaatt actttttacc
     6421 aaccaaacaa aactggctgg atgtgattcg cgcctccagt gcgatacctg gcttttatcg
     6481 tagcggagtg tcgctggaag gcattaacta cctggatggc gggatcagtg atgcgattcc
     6541 ggttaaagag gcggcaaggc agggagcaaa aaccttggtc gtcatccgca ccgtgccgtc
     6601 gcaaatgtac tacacgccgc agtggttcaa acgcatggaa cgctggctgg gtgacagtag
     6661 cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc agctattgtg atattcagca
     6721 atttattgag aaaccaccgg gcaaactgcg gatattcgaa atttatccgc cgaagccatt
     6781 gcatagtatc gcgcttggca gtcggattcc ggcgctgcgt gaagactata aacttgggcg
     6841 tttatgcggt cgttatttcc tggcaacggt tggcaagcta ttaactgaaa aagcgccgct
     6901 aacccgccat ctggtgccag tggtgacgcc ggaatcgatt gtcattccgc ctgcgccagt
     6961 cgccaacgat acgctggttg ccgaagtgag cgatgctccg caggcgaacg acccgacatt
     7021 taacaatgag gatctggctt gatttgccgt tttatcgaca cccactgcca ttttgatttc
     7081 ccgccgttta gtggcgatga agaggccagc ctgcaacgcg cggcacaagc gggcgtaggc
     7141 aagatcattg ttccggcgac agaggcggaa aattttgccc gcgtgcaggc gttagcggaa
     7201 aaatatcaac cgctgtatgc cgcattgggc ttgcatcctg gtatgttgga aaaacatagc
     7261 gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc gtccggcgaa ggtggtggcg
     7321 gtgggggaga tcggtctgga tctcttcggc gacgatccgc aatttgagag gcagcagtgg
     7381 ttactcgacg aacaactgaa actggcgaaa cgctacgatc tgccggtgat ccttcattca
     7441 cggcgcacgc acgacaaact cgcgatgcat cttaaacgcc acgatttgcc gcgtactggc
     7501 gtggttcacg gtttttccgg cagcctgcaa caggctgaac ggtttgtaca gctgggctac
     7561 aaaattggcg taggcggcac tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc
     7621 gcaaaactac cgctggcatc gttgttgctg gaaaccgacg cgccggatat gccgctcaac
     7681 ggctttcagg ggaagcctaa tcgcccggaa caggccgcgc gggtgtttgc cgtgctttgc
     7741 gagttacgcc cggaacccgc ggatgaaatt gccgaagtgt tgcttaataa cacgtatacg
     7801 ttgtttaacg tgccgtaggc cggacaaggc gtttacgcca catccggcat gtcacaaata
     7861 cagcgccgga aatatcaacc ggctcacccc gcgcaccttt aacgcatcag ccaactgctc
     7921 aacatcttcc ggtgtggcgc tcggccagct ttgcgcttcg ccatacacgc cgtgggcatg
     7981 aaacgcgttc aggcgtaccg gaacatcgcc aagtaccttg ataaacgccg ccagttcttc
     8041 gatgtgttgc aaataatcca cctggccagg gattaccagc aaacgcagtt ccgccagctt
     8101 gccgcgctct gccagcaaat agatgctgcg cttaatctgc tgattatcgc gtccggttaa
     8161 ctgttgatga cattcgctcc cccacgcttt gagatcgagc atcgcgccgt cgcacaccgg
     8221 gagcaatttt tcccagccgg tttcgctcaa cataccgtta ctgtccacca gacaggtgag
     8281 atggcacagt tgcggatcat ttttgatagc agtaaacagc gccaccacaa acggtagctg
     8341 ggtcgtggct tcgccgccgc ttaccgttat cccttcgata aacagcactg ctttgcggat
     8401 atggctaagc acctcatcca cgctcatgga ctgcgccatt ggcgtggcat gttgcggaca
     8461 catcttcagg caggtatcac attgctcgca gaccacagcg ttccacacca ctttgccgtc
     8521 aacaatctgc aacgcctgat gcggacactg tggcacgcac tccccgcagt cattgcaacg
     8581 tcccatcgtc cacggattgt gacagttttt gcagcgcaga ttgcagccct gcaaaaacag
     8641 agccagacga ctgcctggcc cgtcaacgca ggagaagggg ataatcttac tgactaaagc
     8701 gcatctgctg ttcatggctt atcacgcgcg gctggcgttc cagaatacga gtgttacgtg
     8761 cggcctcttc gcccagccag gtggtgttgg tacgtgaacc ttcggcgcga tatttttcta
     8821 aatccgacaa acgcaccata taaccggtaa cgcgaaccag atcgttaccg ctgacattgg
     8881 cggtaaattc acgcattccg gctttaaagg caccgaggca aagctgtacc agcgcctgcg
     8941 gattacgttt gatggtttcg tcgagcgtca gaatgtcgct gatgccggaa taataataag
     9001 catgatgcgg ggcgacggtt tgcagatggg tgatcggatc tggctcatca ccatacggca
     9061 aacgtgcgcc cggcgtggtg ccgatatcgg aacttatccc tgactgtgcg tgtaacatgg
     9121 cgcgtttttg ccagccatat ttcacggggg tattggcgac aaactccgcc agttgcgcgc
     9181 tgatgcgata acccacttca tttgcggcgg cttctttacc gtagcgcgcg gcaatccctt
     9241 ctttctcaca cagcaagtta accgcttccg ccagcccata catgccaaac attggcacaa
     9301 aacgttcagg gttaatcagc ccttctttca ccaggaagct attctcaaag aagtgtgatt
     9361 gttgatagag gaattcacac cgcgcatcga tgatggcgat ctgctgctgg cagtagtgcg
     9421 gtagagtgcg cgcaaagaag tcatccagcg attcgctgcg ctcggcaatg gctttcaggt
     9481 taaggcgtac cagcgtgctg ccaccacccg ccagcggcag tgagttgtaa cagctcacaa
     9541 tcccgtagcc cccttttgtg aaaattttat catgcaccgg accgttggcg atgtgcggtt
     9601 tgctacattc acagatgttc ttcgccactt cctgcagcag gtcatcaggg gtgatttcag
     9661 gatcgtagat aaaggtcagg ttcggtgaaa cctgcttcag ctctgcatct gcacgtaaga
     9721 ttgcacgggt gatgggcgaa tcagacgggc cgatattggc gtgcataaag gcgtctggca
     9781 gggttctgtc gaggtaacgc cagaaacgtt ttattcgaat atcgatctcg tcttgtgtta
     9841 gaattctaac atacggttgc aacaacgcat ccagttgccc caggtagacc ggcatcgatg
     9901 tgaccgacgg tacgtggtgg taaagaatgg tcagcagaga gagtgcatca tcaagatctt
     9961 tcgcgccttc cagctccagc cattcggaac cgttcgccag aaaacgggcg taatcgggta
    10021 agacatagcg cggtttgtac ggcgcatgac cttcaaacat atcgcagatt acaccctcat
    10081 ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg cagattgttt tctgcttcca
    10141 gcgccagaaa atggcgcttc tgctccgggc taagtaccgg actggtgaca atttgctggc
    10201 aacgttgttg cagtgcaatc tcatgagaag tgggcatctt cttttccttt tatgccgaag
    10261 gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg atcctgatgc gtttgccacc
    10321 actgacgcat tcatttgaaa gtgaattatt tgaaccagat cgcattacag tgatgcaaac
    10381 ttgtaagtag atttccttaa ttgtgatgta tatcgaagtg tgttgcggag tagatgttag
    10441 aatactaaca aactcgcaag gtgaatttta ttggcggcaa gccaggagaa tgaaatgact
    10501 gatctgaaag caagcagcct gcgtgcactg aaattgatgg acctgaccac cctgaatgac
    10561 gacgacaccg acgagaaagt aattgctctg tgtcatcagg ccaaaactcc ggtcggcaat
    10621 accgccgcta tctgtatcta tcctcgcttt atcccaattg ctcgcaaaac gctgaaagag
    10681 cagggtaccc cggaaatccg tatcgctacg gtaaccaact tcccacacgg taacgacgac
    10741 atcgacatcg cgctggcaga aacccgtgcg gcaatcgcct acggtgccga tgaagttgac
    10801 gtagtgttcc cgtaccgtgc gctgatggca ggtaacgagc aggttggttt tgacctggtg
    10861 aaagcctgta aagatgcttg tgcggcagcg aatgtactgc tgaaagtgat catcgaaacc
    10921 ggcgaactga aagacgaagc gctgatccgt aaagcgtctg aaatctccat caaagcgggt
    10981 gctgacttca tcaaaacctc taccggtaaa gtggcagtga acgcgacgcc ggaaagcgcg
    11041 cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa aaaccgttgg tttcaaaccg
    11101 gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc tcgccatcgc cgatgaactg
    11161 ttcggcgctg attgggccga tgcgcgtcac taccgctttg gcgcttccag cctgctggca
    11221 agcctgctga aggctctggg ccacggcgac ggtaagagcg ccagcagcta ctaaggcttg
    11281 atttgccgga tgacgcagcg atgctcatcc ggttccccga ttacctcgtc tcttttccac
    11341 gggaggttac cttgtttctc gcacaagaaa ttattcgtaa aaaacgtgat ggtcatgcgc
    11401 tgagcgatga agaaattcgt ttctttatca acggtattcg cgacaacact atctccgaag
    11461 ggcagattgc cgctctcgcg atgaccattt tcttccacga tatgacaatg cctgagcgtg
    11521 tctcgctgac catggcgatg cgagattcag gaaccgttct cgactggaaa agcctgcatc
    11581 tgaatggccc aattgttgat aaacactcca ccggtggcgt cggcgatgtg acttcgctga
    11641 tgttggggcc gatggtcgca gcctgcggcg gctatattcc gatgatctcc ggtcgcggcc
    11701 tcggtcatac tggcggtacg ctcgacaaac tggaatccat ccctggcttc gacattttcc
    11761 cggatgacaa ccgtttccgc gaaattatta aagacgtcgg cgtggcgatt atcggccaga
    11821 ccagctcact ggctccggcg gataaacgtt tctacgcgac ccgtgatatt accgcaaccg
    11881 tggactccat cccgctgatc accgcctcta ttctggcgaa gaaactggcg gaaggtctgg
    11941 acgcgctggt gatggacgtg aaagtgggta gcggcgcgtt tatgccgacc tacgaactct
    12001 ctgaagccct tgccgaagcg attgttggcg tggctaacgg cgctggcgtg cgcaccaccg
    12061 cgctgctcac cgatatgaat caggtactgg cctccagtgc aggtaacgcg gttgaagttc
    12121 gtgaagcggt gcagttcctg acgggtgagt atcgtaaccc gcgtctgttt gatgtcacga
    12181 tggcgctgtg cgtggagatg cttacctccg gcaaactggc gaaagatgac gccgaagcgc
    12241 gcgcgaaatt gcaggcggtg ctggacaacg gtaaagcggc agaagtcttt ggccgtatgg
    12301 tggcggcaca aaaaggcccg accgacttcg ttgagaacta cgcgaagtat ctgccgacag
    12361 cgatgctgac gaaagcagtc tatgctgata ccgaaggttt tgtcagtgaa atggataccc
    12421 gtgcgctggg gatggcagtg gttgcaatgg gcggcggacg ccgtcaggca tctgacacca
    12481 tcgattacag cgtcggcttt actgatatgg cgcgtctggg cgaccaggta gacggtcagc
    12541 gtccgctggc ggttatccac gcgaaagacg aaaacagctg gcaggaagcg gctaaagcgg
    12601 tgaaagcggc aattaaactt gccgataaag caccggaaag cacaccaact gtctatcgtc
    12661 gtatcagtga ataacggtat actgatctga tcatttaaat ttgaagcact gagtacggag
    12721 aacatatgaa acgtgcattt attatggtgc tggactcatt cggcatcggc gctacagaag
    12781 atgcagaacg ctttggtgac gtcggggctg acaccctggg tcatatcgca gaagcttgtg
    12841 ccaaaggcga agctgataac ggtcgtaagg gcccgctcaa tctgccaaat ctgacccgtc
    12901 tggggctggc gaaagcacac gaaggttcta ccggtttcat tccggcggga atggacggca
    12961 acgctgaagt tatcggcgcg tacgcatggg cgcacgaaat gtcatccggt aaagataccc
    13021 cgtctggtca ctgggaaatc gccggtgtgc cggttctgtt tgagtgggga tacttctccg
    13081 atcacgaaaa cagcttcccg caagagctgc tggataaact ggtcgaacgc gctaatctgc
    13141 cgggttacct cggtaactgc cactcttccg gtacggtcat tctggatcag ctgggcgaag
    13201 agcacatgaa aaccggcaag ccgattttct atacctccgc tgactccgtg ttccagattg
    13261 cctgccatga agaaactttc ggtctggata aactctacga actgtgcgaa atcgcccgtg
    13321 aagagctgac caacggcggc tacaacatcg gtcgtgttat cgctcgtccg tttatcggcg
    13381 acaaagccgg taacttccag cgtaccggta accgtcacga cctggccgtt gaaccgccag
    13441 caccgaccgt gctgcagaaa ctggttgatg aaaaacacgg ccaggtggtt tctgtcggta
    13501 aaattgcaga catctacgcc aactgcggta tcaccaaaaa agtgaaagcg actggcctgg
    13561 acgcgctgtt tgacgccacc atcaaagaga tgaaagaagc gggtgataac accatcgtct
    13621 tcaccaactt cgttgacttc gactcttcct ggggccaccg tcgcgacgtc gccggttatg
    13681 ctgcgggtct ggaactgttc gaccgccgtc tgccggagct gatgtctctg ctgcgcgatg
    13741 acgacatcct gatcctcacc gctgaccacg gctgtgatcc gacctggacc ggtactgacc
    13801 acacgcgtga acacattccg gtactggttt acggcccgaa agtaaaaccg ggctcactgg
    13861 gtcaccgtga aaccttcgcg gatatcggcc agactctggc aaaatatttt ggtacttctg
    13921 atatggaata tggcaaagcc atgttctgat ggatttgggc ggagcgttga ctccgccttt
    13981 gttatgtcac aaaaaggata aaacaatggc taccccacac attaatgcag aaatgggcga
    14041 tttcgctgac gtagttttga tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac
    14101 tttccttgaa gatgcccgtg aagtgaacaa cgttcgcggt atgctgggct tcaccggtac
    14161 ttacaaaggc cgcaaaattt ccgtaatggg tcacggtatg ggtatcccgt cctgctccat
    14221 ctacaccaaa gaactgatca ccgatttcgg cgtgaagaaa attatccgcg taggctcctg
    14281 tggcgctgtt ctgccacacg taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac
    14341 cgattccaaa gttaaccgca tccgttttaa agaccatgac tttgccgcta tcgctgactt
    14401 tgacatggtg cgtaacgcag tagatgcagc taaagcactg ggcattgatg ctcgcgttgg
    14461 taacctgttc tccgctgacc tgttctactc tccggacggc gaaatgttcg acgtgatgga
    14521 aaaatacggc atcctcggcg tggaaatgga agcggctggt atctacggcg tcgctgcaga
    14581 atttggcgcg aaagccctga ccatctgcac cgtgtctgac cacatccgta ctcacgagca
    14641 gaccactgcc gctgagcgtc agaccacctt caacgacatg atcaaaatcg cactggaatc
    14701 cgttctgctg ggcgataaag agtaattgtg tttcgctgaa aggcgattgc cttgtgaagc
    14761 cggagcggga gactgctccg gctttttagt atctattcat caatagcaac tgatttatag
    14821 a
//
LOCUS       DARNVM010000118        14682 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.118, whole genome shotgun sequence.
ACCESSION   DARNVM010000118 DARNVM010000000
VERSION     DARNVM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.118"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(156..908)
                     /gene="yecC"
                     /locus_tag="SI621_003309"
     CDS             complement(156..908)
                     /gene="yecC"
                     /locus_tag="SI621_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HEI4065981.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLEGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(905..1573)
                     /gene="tcyL"
                     /locus_tag="SI621_003310"
     CDS             complement(905..1573)
                     /gene="tcyL"
                     /locus_tag="SI621_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HEI4065982.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAGTIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1588..2574)
                     /gene="dcyD"
                     /locus_tag="SI621_003311"
     CDS             complement(1588..2574)
                     /gene="dcyD"
                     /locus_tag="SI621_003311"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEI4065983.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2679..3479)
                     /gene="tcyJ"
                     /locus_tag="SI621_003312"
     CDS             complement(2679..3479)
                     /gene="tcyJ"
                     /locus_tag="SI621_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4065984.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3567..4118)
                     /gene="fliZ"
                     /locus_tag="SI621_003313"
     CDS             complement(3567..4118)
                     /gene="fliZ"
                     /locus_tag="SI621_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HEI4065985.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTRTGLVQKSSSLPASDIY"
     gene            complement(4164..4883)
                     /gene="fliA"
                     /locus_tag="SI621_003314"
     CDS             complement(4164..4883)
                     /gene="fliA"
                     /locus_tag="SI621_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEI4065986.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5203..6873)
                     /locus_tag="SI621_003315"
     CDS             complement(5203..6873)
                     /locus_tag="SI621_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HEI4065987.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTGFNVNGEGETANTAATLKDMVGLKLDNTGVTTAGVNRYIADKAVASS
                     TDILNAVAGVDGSKVSTEADVGFGAAAPGTPVEYTYHKDTNTYTASASVDATQLAAFL
                     NPEAGGTTAATVSIGNGTTAQEQKVIIAKDGSLTAADDGAALYLDTNGNLSKTNAGTD
                     TQAKLSDLMANNAKAKTVITTDKGTFTANTTNDGFDGVDISVDASTFANAVKNETYTA
                     TVGVTLPATYTVNNGTAASAYLVDGKVSQTPAEYFAQADGTITSGENAATSKAIYVSA
                     NGNLTTNTTSESEATTNPLAALDDAIASIDKFRSSLGAIQNRLDSAVTNLNNTTTNLS
                     EAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7139..8545
                     /gene="fliD"
                     /locus_tag="SI621_003316"
     CDS             7139..8545
                     /gene="fliD"
                     /locus_tag="SI621_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEI4065988.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKEPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8570..8980
                     /gene="fliS"
                     /locus_tag="SI621_003317"
     CDS             8570..8980
                     /gene="fliS"
                     /locus_tag="SI621_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEI4065989.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESNDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8980..9345
                     /gene="fliT"
                     /locus_tag="SI621_003318"
     CDS             8980..9345
                     /gene="fliT"
                     /locus_tag="SI621_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HEI4065990.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVEPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSMQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9423..10910
                     /gene="amyA"
                     /locus_tag="SI621_003319"
     CDS             9423..10910
                     /gene="amyA"
                     /locus_tag="SI621_003319"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEI4065991.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10944..11357)
                     /locus_tag="SI621_003320"
     CDS             complement(10944..11357)
                     /locus_tag="SI621_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEI4065992.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11544..12749
                     /gene="yedE"
                     /locus_tag="SI621_003321"
     CDS             11544..12749
                     /gene="yedE"
                     /locus_tag="SI621_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HEI4065993.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12746..12979
                     /gene="yedF"
                     /locus_tag="SI621_003322"
     CDS             12746..12979
                     /gene="yedF"
                     /locus_tag="SI621_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HEI4065994.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13086..13754
                     /locus_tag="SI621_003323"
     CDS             13086..13754
                     /locus_tag="SI621_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEI4065995.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFVDG
                     WFEWKKEGDKKQPYFIYRADGQPVFLAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAAL
                     IQPV"
     gene            13865..14344
                     /locus_tag="SI621_003324"
     CDS             13865..14344
                     /locus_tag="SI621_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEI4065996.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            complement(14482..>14682)
                     /locus_tag="SI621_003325"
     CDS             complement(14482..>14682)
                     /locus_tag="SI621_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEI4065997.1"
                     /translation="EVVPAIHSNLKLLFIECYPIKEEFLMPWTLTDITYEISQRKYIV
                     TNSADYDKYSDIVKRGVIDGKN"
BASE COUNT         3616 a         3768 c         3604 g         3694 t
ORIGIN      
        1 atataattta tattattcag gcaatgaatt acttttgcaa gccatcgcat tctcttatgt
       61 tattaatgag ttatgctgat ttgttaagca gatttatcac gcttgaaatg gcgtcccgcc
      121 ccgacaggtg aatcgtcggg gctgattttt tcttattatt gcagcagaaa cttctcgagg
      181 aactggcggg tgcgaggctg ctgaggatcg gcaaataacg cttttgcggc cccctgctcg
      241 actatccgcc cctggtccat aaagatcgcc cggtccgcaa catcccgggc aaagctcatt
      301 tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca gctggcggat ggtgttcagg
      361 acttcgccca ccagctctgg atccagcgcc gacgttggct cgtcaaacaa aatcacctca
      421 ggacgcatag ccagcgctcg cgcaatcgcg acacgctgct gttgaccgcc agacaaacga
      481 cgtggatagc tggtttcttt accttccagc ccaactttcg ccagcagctc gcgagcgcgc
      541 gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat aatgttttcc
      601 agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac gtgctgacgc
      661 aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat ggtgatatcg
      721 ccaacggtga tggttcccgc ttccggttgt tccagcagat ttatgctgcg tagcaacgtg
      781 gttttgccgg aaccacttgg cccgataatt gccaccactt cgccaggctt cacctcaagg
      841 tcgataccgt gcagcaccgt ctgaccgtgg aattttttca ccaggttctt aacttcaatg
      901 gcactcattt tggttctctc tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg
      961 tcgataatac tgtcgccatg atccagtaga tgagcgaagc cgccaggtac atggtgaaca
     1021 cctccagcgt acgcgaggta atcaactgcg cctgacggaa cagctccggc acctggattg
     1081 tcccggccag cgacgtatct tttaccaggc tgataaagct gttcgacagc ggcggcaacg
     1141 ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt ctgccacggc gtcataccaa
     1201 tactggcagc agcttcccac tgacctttat caatagaaga aatggcggcg cgcagcgttt
     1261 cggcagcata ggcggcagtg tttagcgaca gaccaatcat tgctgacgga atgggatcta
     1321 gttcgatgcc aaactgcggc aagccgtaat agatcataaa cagttgggcg atgagcggcg
     1381 taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag attggcgaaa
     1441 ggcgcatcag cgcgagaata aacccgagca gtaagccaaa aaacatgccg ccgatactga
     1501 gttgcagcgt atacccagcc cctttcagca ggaacggcaa ggaatcaata accagttgta
     1561 tactttcttg catgagcgtt tccggagcta aacgtgagga tgataggcga acagcgcagg
     1621 cgcgccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt tctgactgat
     1681 accgtcaatc agccccgcca tcgcttttcc ggtatacaca ggatcaagca gaatgccttc
     1741 cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg
     1801 tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca gttctttcgc
     1861 aatcgcctgt tggaggttaa ccactttcgg caattgatcg gcaacggaac gcgacacggt
     1921 cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca gtccggcgtg
     1981 agttccggca ctgcccgatg cgaccaccac cgacgaaata ttaaccgccc cttcacactg
     2041 ttgcgcgatt tccagcgcac tctccacata acctagcgcg cccagagcat tagaaccgcc
     2101 aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca gctcttccag
     2161 ttgggcattg ggatcggtca gtgcgtcgca catttcaatc tgggtattga acagatccag
     2221 caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag gattttccag
     2281 cagcgccacg cagtgcagac cgagtttcgc agcgactgcg gcagtctggc gcacatggtt
     2341 agactggatc gccccagcag taatcagcgt atcggcacct tcacgcagag catctgccgc
     2401 gagaaattcc agcttacgta atttattgcc gcccattgcc atgggagtga cgtcatcccg
     2461 tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt cgagcggcgt
     2521 tggcgcgccg ataaactcca gccgtggaaa acgggttaaa ttatgcagtg gcataacagc
     2581 ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt aaataaaaaa
     2641 ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac atcagcacca
     2701 aaccattttt cggaaagcgc ttgcagagtg ccgtcttttt gcatttccgc aattgcatca
     2761 ttcactgctt tcagcaggtc ttcatttcct ttacgcaacg ccacgccaga ctcctgacgg
     2821 gagaatgctt caccggttac tgccagcgta tcgttggttt ttttcaccag atccagcgcc
     2881 gccagacggt caacgaggat cgcatcgata cggcctacgc ggagatcctg atatttggtc
     2941 gggtcatcat cataggtacg cacatcgacg ccctgaacat tctgacgaag ccactcttca
     3001 tagttggtgc ccagaccgac acccactttt ttgcctttca gatcagcagc cgttttaatg
     3061 gtgccttcgt tacctttttt caccagcgcc tgaataccag aaatggtgta cggggtagag
     3121 aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct gattaatcac cacatcaatg
     3181 cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag tgacgcctca
     3241 acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt taatttgccg
     3301 tcatccccct gaaaactgaa cggcggataa gttccttcca gccctaccag cagcgtgccg
     3361 cgctctttga ctttattaag cagaccttca tctgcaaaac ttttaacgct catgcccgca
     3421 accagcgcca cggccatcac acccatcaat gcctgacgtc ccagatgtgc taatttcata
     3481 ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt aaacacaaaa
     3541 acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag gctggaggat
     3601 ttctgcacaa gtccggtacg cgtttgacgc tgatagtgtt gatacttccg taacgcgatg
     3661 cggtaattgt tggtgctggt ggtgggcagc catggggcga ggatttcatc aaggaaacca
     3721 tcctgcagct gatcgaggga aatattttgc tcgtgtagat gattacccaa acggcgcaga
     3781 cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa aagaaattgc
     3841 ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc accgcagaat
     3901 cggcacaatg ccgcccatga tttttgttcc acttgccagc cattttcatc aagcagcatg
     3961 tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaacgag ggtaatgcgg
     4021 tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc tttaagatag
     4081 cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg gtagtcaaaa
     4141 ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa ccgtttaatg
     4201 gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc gccaatctct
     4261 ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg ctccggcaac
     4321 gtttcgatag cttccatcac ccgctggcgc agattactgt ccagcagctg ttgtagcggg
     4381 ttttctcgct gatgatcatc agtaaccagt tcaatgctat cgccgtgctc ttcgcgccac
     4441 tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg ataatcggca
     4501 atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttacggcc aagttcctgc
     4561 tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct gcgcggcacc
     4621 cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac tgcgtaagtt
     4681 gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa cccaatgccg
     4741 cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg caggcgcaat
     4801 gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc cattacacct
     4861 tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt atcggcatga
     4921 ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag gggttatttg
     4981 ggggttaccg gtaaattgcg gacagaaaaa accccgccgg tggcggggaa gcacgttgct
     5041 ggcaaattac cattcatgtt gccggatgcg gcgtaaacgc cttatccggc ctacaaaaat
     5101 gtgcaaattc aataaattgc aattcacctt gtaggcctga taagcgcagc gcatcaggca
     5161 atttggcgtt gccgtcagtc tcagttaatc aggttacgac gattaaccct gcagcagaga
     5221 cagaacctgc tgcggtacct ggttagcttt tgccagcacg gagttaccgg cctgctgaat
     5281 aatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga
     5341 ctgcgcttcg gacaggttgg tagtggtgtt gttcaggttg gtgaccgcgg aatccagacg
     5401 gttctggata gcaccaaggg atgaacggaa tttgtcgatg gacgcgatag cgtcatccaa
     5461 tgctgccagc gggttggtag tcgcttcaga ttcactcgtt gtgttagtcg ttaagttacc
     5521 attggcactt acatagatag ctttactggt agccgcattt tcaccactag taatagtgcc
     5581 atcagcttga gcaaaatact cggcaggagt ttggctcact tttccatcga ctaaatacgc
     5641 tgatgcagca gtgccattat tgactgtata tgtcgctggt aaagttacac caacagttgc
     5701 agtgtaagtc tcatttttaa cggcattagc aaacgttgaa gcatcaacag aaatatctac
     5761 cccatcaaaa ccatcgtttg tcgtattagc agtaaatgta cctttatctg ttgtaatgac
     5821 tgtttttgcc ttagcattgt ttgccattaa gtcagacagt ttagcttgag tatcagtgcc
     5881 tgcgttagtt ttacttaagt taccattagt atcaagatag agagcggcac cgtcatcagc
     5941 agcagttaaa gaaccatctt tagcaataat gactttttgc tcttgagctg ttgtaccgtt
     6001 gccaatactt actgttgcag cagtggtacc acccgcttca ggattcagga atgccgccag
     6061 ttgagtcgca tcaactgaag cagaagccgt atatgtgtta gtatctttat gataagtata
     6121 ttccactggc gtaccagggg cagccgcacc aaaaccaaca tctgcctccg tggaaacttt
     6181 actgccatca acaccagcta ccgcattcaa aatatccgtg ctacttgcga cggctttgtc
     6241 agcaatatat ctattaactc cagctgtagt gacccccgta ttatcgagtt ttaaaccaac
     6301 catatcttta agtgttgcag cagtattggc tgtttcacct tcgccattca cgttaaaacc
     6361 ggtcagcttc aatgtagaag agtcgatctt tttcagatca atcgtgatgg tttcaccatc
     6421 gttcgcgccg acctgaattt tcatcgagcc atcttttgac agtacgttca cgccgttgaa
     6481 ctgggtctgg ccggatacgc ggtcaatttc atcaagacgg gatttgattt cgtcctgaat
     6541 ggagtccagg tcagaggtgg agttagttcc ggtcgtcgcc tgaaccgtca gttcacgaat
     6601 acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg caacagaaat
     6661 accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag taaaacggtt
     6721 agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag
     6781 acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag
     6841 cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt
     6901 tgattacgta ttgggtttcc acccgtcggc tcaatcgccg tcaaccctgt tatcgtctgt
     6961 cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt cgccgaaata
     7021 ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc
     7081 aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag gaaatcgcat
     7141 ggcaagtatt tcatcgctgg gagtcgggtc aggcctggat ttaagttcaa tccttgatag
     7201 cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat cgtcgtttac
     7261 cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa
     7321 tactgcattg tctaaagccg atcttttttc cgccaccagc actaccagca gcaccaccgc
     7381 gttcagtgcc accactgcgg gtaacgccat cgccgggaaa tacaccatca gcgtcaccca
     7441 tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gacgatacga aaacggcgat
     7501 cgccaccagc gacagcaaac tcaccattca acaaggcggc gacaaagagc cgatcaccat
     7561 tgatatcagc gcggctaact cgtcgttaag cgggatccgt gatgccatca acaacgcaaa
     7621 agcaggtgta agcgcaagca tcattaacgt gggtaacggt gaatatcgtc tgtcagtcac
     7681 atcgaatgac accggccttg ataatgcgat gacgctctcg gttagcggtg atgatgcgct
     7741 acaaagtttt atgggctatg acgccagtgc cagtagcaac ggtatggagg tctcggtcgc
     7801 cgcccagaat gcgcagctga cggtcaacaa cgtcgccatt gagaacagca gcaacaccat
     7861 cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg gcaaccagac
     7921 gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact gggtgaatgc
     7981 ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg tagatgcggg
     8041 agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc tgcggacgat
     8101 tcagacgcag ttgaaatcga tgctgagtaa taccgtcagt tcttccaact ataaaacgct
     8161 ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggagctgg atgccgacaa
     8221 actcaccgct gcattgaaaa aagatgccag cggcgtaggt gcattgattg ttggcgatgg
     8281 taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc tttcgacaac
     8341 aggcattatt aaagccgcta ccgatggcgt tagtaagacc ctgaataagt taactaaaga
     8401 ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag aacaatttac
     8461 ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa cgcagcagtt
     8521 cgaaaacaac agtaattcca agtaatcaat attcatcggg agacaggtca tgtacgcggc
     8581 aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc gccgtaatga gcgccagcca
     8641 gcagcagctg gtcaccatgc tatttgatgg agtgctgagc gcactggtta gagcgcgcct
     8701 gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag cgatcaacat
     8761 tattgagaac ggactgcggg tgagtcttga tgaagagagc aatgacgaat taacccaaaa
     8821 cttgattgct ctttatagct atatggtcag gcgtttgctg caagccaatt tacgcaacga
     8881 tgtctccgca gtcgaagaag tggaagcatt aatgcgcaat attgccgatg cctggaaaga
     8941 gtcgttactc tccccttctt tgattcagga cccagtctga tgaaccacgc accgcatttg
     9001 tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct ggcaacggaa
     9061 gaacagtggg acgaactcat cgccagcgaa atggcgtatg tgaatgcggt gcaggagatt
     9121 gcacacctga ctgaagaggt tgagccgtcc accacgatgc aggagcaact ccgcccgatg
     9181 ctgcgcctga ttctcgataa cgaaagcaag gtgaagcagt tattacagat tcggatggat
     9241 gaactggcga aactggtcgg tcagtcatcg atgcaaaaat cggtgttaag tgcctatggc
     9301 gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt aacgagccga
     9361 tggctcgcga ataatccgat tacggctacg cttctaatgt tccccttgaa tggagtcgaa
     9421 gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa ggcggtaagc
     9481 tctggcctga actggccgag cgcgccgacg gttttaatga tattggtatc aatatggtct
     9541 ggttgccgcc cgcctataaa ggcgcatcgg gcgggtattc ggtcggctac gactcctatg
     9601 atttatttga tttaggcgag tttgatcaga aaggcagcat ccctactaaa tatggcgata
     9661 aagcacaact gctggccgcc attgacgctc tgaaacgtaa tgacattgcg gtgctgctgg
     9721 acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc gtgcagcgtg
     9781 taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgcgaa ggctggacgc
     9841 gttacacctt ccccgcccgt gccgggcaat actcgcagtt tatctgggat ttcaaatgtt
     9901 ttagcggtat cgaccatatc gaaaaccctg atgaagatgg catttttaaa attgttaacg
     9961 actacaccgg cgaaggctgg aacgatcagg ttgatgatga actcggtaat ttcgattatc
    10021 tgatgggcga gaatatcgat ttccgcaatc atgccgtgac ggaagagatt aaatactggg
    10081 cgcgctgggt gatggaacaa acgcaatgcg acggttttcg tcttgatgcg gtcaaacata
    10141 ttccggcgtg gttttataaa gagtggatcg aacacgtaca agaagttgca ccaaagccgc
    10201 tatttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg tatattgatc
    10261 aggtggaagg caaaaccatg ctgttcgatg caccgctgca gatgaaattc catgaagcat
    10321 cgcgcatggg gcgcgactac gacatgacgc agattttcac gggtacatta gtggaagccg
    10381 atccttttca cgccgtaaca ctcgttgcca accacgacac ccaaccgttg caagccctcg
    10441 aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc tctaattttg ttgcgggaaa
    10501 atggcgttcc ttcggtattc tatccggacc tctatggcgc gcattacgaa gatgttggcg
    10561 gtgacgggca aacctatccg atagatatgc caataatcga acaacttgat gagttaattc
    10621 tcgcccgtca gcgtttcgcc cacggtgtac agacgttatt tttcgaccat ccgaactgca
    10681 ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtt atgtcaaacg
    10741 gggatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa acctggcgtg
    10801 attttctggg gaatcggcaa gagagcgtag tgaccgacga aaacggcgaa gcgaccttct
    10861 tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa attcatcccc
    10921 ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc tttatccagc
    10981 gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc atcgtactca
    11041 atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac gttagtgaga
    11101 tcatctgaca tcaatgtcaa cttgcccggc acggcaatca cccgctgcca ctgacggcaa
    11161 tccagagtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg actgaccagc
    11221 gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc gggggttttc
    11281 acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag cgcgcctgca
    11341 attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt ggcattaaag
    11401 ccctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta ttatttttgt
    11461 gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcgactcct gtttcaaacc
    11521 ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc tggttgatta
    11581 aattctgggc gcccatccct gcggtcatcg cggcgggtat tctctccact tactattttg
    11641 gcatcactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc ggtcagctcc
    11701 tgcagctgtt cggcgtccat gctgaagagt ggggttattt taaaattatc catctggaag
    11761 gatcgccatt aacccgcatc gacgggatga tgatcctcgg tatgtttggc ggctgctttg
    11821 ctgcagcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt atccgcatta
    11881 tgcaagccat tattggcggc attatcgctg gttttggcgc gcgtctggca atgggctgta
    11941 acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg ttctttgcca
    12001 tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc atcttccgta
    12061 ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa ccggatcagg
    12121 cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc tgggcactgc
    12181 tgacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc ggctttggtt
    12241 tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg tggatcaccg
    12301 gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc atcgggatct
    12361 tcagttacgt gcagttaggc gttgaaccca aaatcatgtg ggcgggacca aacgcggtaa
    12421 ttggtggttt actgtttggt tttggcatcg tgctggcagg cggctgcgaa accggctgga
    12481 tgtatcgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc aacgtgatcg
    12541 gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc accgactggg
    12601 acaaaatcaa cctactgaaa acctttggtc cgatgggtgg cctgctggtg acatatttgc
    12661 tgttgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc cgccgtgcgg
    12721 caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc gccttgatat
    12781 ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc cgcaattaaa
    12841 aaaaggtgag atcctggaag ttgtgagcga ctgtccgcag tcgatcaata atattccact
    12901 ggatgcgcgt aatcacggct atacggtgct ggatattcag caagacgggc cgaccattcg
    12961 ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga acatcgttgc
    13021 ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga tctcaggagg
    13081 ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga agattacctt gcgcttctcg
    13141 cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga tacaacgtcg
    13201 cgccgggaac caaagttctt ctactcagtg aacgtgatga acaccttcat ctggatccgg
    13261 ttttttgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag
    13321 aaactgccgc aaccagtcgc atgtttaaac cgctctggca acatggtcgg gcaatctgtt
    13381 ttgtcgatgg ctggtttgag tggaaaaaag aaggcgacaa aaagcagcct tattttatct
    13441 atcgtgctga tggacaacct gtttttctgg ccgcgatagg tagcacaccg ttcgagcgcg
    13501 gtgacgaagc cgaaggattt ttgattgtca ctgcagcagc agaccagggt ctggtagata
    13561 ttcatgaccg ccgcccactg gtactgtcgc cagaagctgc gcgggaatgg atgcggcaag
    13621 atattggcgg taaagaagcc tcagaaatcg ctaccagtgg ctgtgtgcca gcaaaccagt
    13681 tcacctggca cccggtatcg cgcgcggtgg gtaatgtcaa aaaccagggc gcggcgttaa
    13741 ttcaacctgt ttgacaagcc tgaagattat tgatcaacaa tactgcgtca taagaaatct
    13801 ctattagaca aagatttcat tacctgttgg catattgcaa aaataacacc aatgcggaat
    13861 cgttatgttc acgattaaaa cagatgatct cacccatcca gcagtgcaag cattagtggc
    13921 ttaccatatt tccggcatgc tacagcagtc tccccccgaa agcagtcatg ctttagatgt
    13981 gcaaaaatta cgtaacccga cagtgacatt ctggtcagta tgggaaggcg aacaactcgc
    14041 aggaattggt gcgctgaagt tgctggatga taaacatggc gaactgaaat caatgcgtac
    14101 cgcgccaaat tatttacgtc gaggtgtcgc cagtctgatt ttacgccaca ttttgcaggt
    14161 cgcccatgac agatgccttc atcgcctgag cttagaaacg ggtacacagg ctggatttac
    14221 ggcctgccat caactttatt tgaagcatgg tttcgttgat tgcgaaccgt ttgctgatta
    14281 tcaacttgat ccacacagtc gatttttgtc attgacgcta tgcgaaaata atgaattgcc
    14341 atgagccaga cgcagcacat tcttgcattc gacgtgctgc gcctttattc atcaccagcc
    14401 gcatatgtct tgtccttaga cgctccttcc acatgcgcca caagaaacct ctattccagt
    14461 gacacaattg cgcctaacta tttaattctt accatctatt acgccccttt tgactatgtc
    14521 agaatattta tcataatcag cggaattcgt aacaatatat tttcgctgcg atatttcata
    14581 ggtaatgtct gtcaaagtcc acggcattaa aaactcctct ttaataggat aacattctat
    14641 aaaaagtaat tttaaattgc tatgtatcgc agggacaacc tc
//
LOCUS       DARNVM010000119        14650 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.119, whole genome shotgun sequence.
ACCESSION   DARNVM010000119 DARNVM010000000
VERSION     DARNVM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.119"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            371..1489
                     /locus_tag="SI621_003326"
     CDS             371..1489
                     /locus_tag="SI621_003326"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEI4065998.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1555..1803
                     /locus_tag="SI621_003327"
     CDS             1555..1803
                     /locus_tag="SI621_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HEI4065999.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1868..2236
                     /locus_tag="SI621_003328"
     CDS             1868..2236
                     /locus_tag="SI621_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEI4066000.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLSDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2330..2983
                     /gene="nfsB"
                     /locus_tag="SI621_003329"
     CDS             2330..2983
                     /gene="nfsB"
                     /locus_tag="SI621_003329"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HEI4066001.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAQWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3091..4338
                     /gene="ybdG"
                     /locus_tag="SI621_003330"
     CDS             3091..4338
                     /gene="ybdG"
                     /locus_tag="SI621_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HEI4066002.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4406..5782)
                     /gene="pheP"
                     /locus_tag="SI621_003331"
     CDS             complement(4406..5782)
                     /gene="pheP"
                     /locus_tag="SI621_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HEI4066003.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5884..9027)
                     /gene="cusA"
                     /locus_tag="SI621_003332"
     CDS             complement(5884..9027)
                     /gene="cusA"
                     /locus_tag="SI621_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HEI4066004.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPESVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9039..10262)
                     /gene="cusB"
                     /locus_tag="SI621_003333"
     CDS             complement(9039..10262)
                     /gene="cusB"
                     /locus_tag="SI621_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HEI4066005.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNTDEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10278..10610)
                     /gene="cusF"
                     /locus_tag="SI621_003334"
     CDS             complement(10278..10610)
                     /gene="cusF"
                     /locus_tag="SI621_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HEI4066006.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMGEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10634..12016)
                     /gene="cusC"
                     /locus_tag="SI621_003335"
     CDS             complement(10634..12016)
                     /gene="cusC"
                     /locus_tag="SI621_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HEI4066007.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPKQFSLSQNGLVN
                     AADNYQNAGWRTFFIDNQVKTLISEALVNNRDLRMATLKVQEAQAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12173..12856
                     /gene="cusR"
                     /locus_tag="SI621_003336"
     CDS             12173..12856
                     /gene="cusR"
                     /locus_tag="SI621_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HEI4066008.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12846..14294
                     /gene="cusS"
                     /locus_tag="SI621_003337"
     CDS             12846..14294
                     /gene="cusS"
                     /locus_tag="SI621_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HEI4066009.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3523 a         3939 c         3607 g         3581 t
ORIGIN      
        1 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
       61 aaagcttcag gcacccgccg ttatcttacc cttcctcagc aaaatagctg tttatttata
      121 cagtgagtcc tgtttcggac ttctcactcc taatttgatc aactgaagct gaacccgttt
      181 ttttctgcat gcctcaatcg tcaaaattct tcgccaccag ccagctctgc cataacttgt
      241 cacattgcaa aagtagccaa attgcttcag tcaccatcac caaaaaaaag aaacattcca
      301 gaacttccgc atctcgccca gccgctccga cttctatact gaatagaaaa cgccaacata
      361 agagaaacct atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga
      421 actggaaatg caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct
      481 gattgacgca gttaaaaata agatcacggc cggagaggta aagcacgata tcaccgaaag
      541 tatgctggag ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcaattttc
      601 agcgatgcag aaagtcgtat tgcaggcagc cgcagatcat catctggaaa tttgcggcgg
      661 tggcacgcac ccgtttcaga aatggcagcg tcaggaggta tgcgataacg aacgctatca
      721 acgaacgctg gaaaactttg gttatctcat tcaacaggcg accgtttttg gtcagcatgt
      781 ccatgttggt tgcgccagtg gcgatgatgc catttatttg ctgcacggcc tgtcacggtt
      841 tgtgccgcac tttatcgccc tttccgccgc gtcgccatat atgcaaggaa cggatacgcg
      901 ttttgcctcc tcacgaccga atattttttc cgcctttccc gataatggcc cgatgccgtg
      961 ggtcagtaac tggcaacaat ttgaagccct gtttcgctgc ctgagttaca ccacgatgat
     1021 cgacagcatt aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga
     1081 ggttcgggta atggataccc cgttaaccct tagccatgcg gtaaatatgg caggattgat
     1141 tcaggctacc gcccactggt tactgacgga acgcccgttt aaacatcagg agaaagatta
     1201 cctgctgtat aaattcaacc gtttccaggc ctgccgctat gggctggaag gcgtcattac
     1261 cgatccgcat actggcgatc gtcggtcact aacggaagac accttgcgtt tactggaaaa
     1321 aatcgcccct tctgcacata aaattggcgc atcgagtgcg attgaggccc tgcatcgcca
     1381 ggtcgtcagc ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggcggctc
     1441 gctgattggg ctggtgaaaa agcattgtga gatctgggct ggtgactaag ccgggaattg
     1501 ccttacagaa gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa
     1561 caccctttag aaaccttgac caccgcagca ggcattttgc tgatggcttt cctttcttgc
     1621 ctgctgctgc ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc
     1681 catctgatgg atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg
     1741 ggcgctattg agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat
     1801 taaacgtcga ttatcgccga acttcgcggc atactttgct tattctcctt cgcctaaagg
     1861 aacgtttatg gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc
     1921 ctttgtcgag ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa
     1981 gatttttatg ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc
     2041 acaaaaatcc ctgttaaatc agcagatata cccgagcatt aaaccaggat atcacatgaa
     2101 taaaaagcac tggatttcgg tttatcccgg cgaggaaatc tccgaagcgt tacttagcga
     2161 tctgatcaac gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag
     2221 agtgcgtcca ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt
     2281 gaaccttgta atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat
     2341 ttctgtcgcc ttaaagcgtc attccactaa ggcatttgat gccagcaaaa aacttacccc
     2401 ggaacaggcc gagcagatca aaacgctact gcaatacagc ccatccagca ctaactccca
     2461 gccgtggcat tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc
     2521 tgccggtaat tacgtgttca acgagcgtaa aatacttgat gcctcgcacg tcgtggtgtt
     2581 ctgtgcaaaa accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc
     2641 cgatggccgc tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt
     2701 cgccgatatg caccgtaaag atctgcatga tgatgcgcag tggatggcaa aacaggttta
     2761 tctcaacgtc ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtgcccat
     2821 cgaaggtttt gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac
     2881 cagtctggtg gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc
     2941 gaaatctcgt ctgccgcaaa acatcacctt aaccgaagtg taattctctc ttgccgggca
     3001 tctgcccggc tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat
     3061 catagtctgc tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat
     3121 ttagcgggta ttgagatcga tcacaccacc tcaatggtga tgattttcgg tattattttt
     3181 ctgaccgccg tcgtggtgca tatttttttg cattgggtgg tactgcggac cttcgaaaaa
     3241 cgtgccatcg ccagttcacg gctatggttg caaatcatta cccagaataa actcttccac
     3301 cgtttagctt ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa
     3361 aaaggcaccg aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat
     3421 gcgctgcttt cagtcttctc gttgctggat gttattttga atctggcgca gaaattcccg
     3481 gcggcatctc agttaccgct gaaagggatt tttcagggga ttaaactgat cggcgcgatt
     3541 ctggtcggca ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc
     3601 ggtcttggtg caatggctgc cgtgctgatg ttggtattta aagatccgat tcttggtctg
     3661 gtggcaggta ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg
     3721 ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt
     3781 aactgggaca ataccattac cactattccc acctggtctc tggtctctga ctcctttaaa
     3841 aactggagcg ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat
     3901 gtcaccagta ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcatttg
     3961 ttaaagcctt atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt
     4021 tctacggagt cggtattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat
     4081 ctgaacgaat atctgcgtaa ccatccgcgg attcgtaaag atatgacttt aatggtacgc
     4141 caactggctc caggtgataa cggtttaccg ctcgagatct atgcgtttac caacaccgtg
     4201 gtgtggctgg aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc
     4261 gaagagtttg gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg
     4321 ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg
     4381 ttttgtcgga tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca
     4441 ggaatacaat ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca
     4501 tcagcagcaa aatcatgccg aggaaggcaa tacaaagata gttgccgaac ggataaagca
     4561 gcgccttaaa ctgtgtttca cgtccctgac gtcgcatcgc cgcgcgaaaa cgcagatgcg
     4621 ccaggcagat cataatccag ttcaacagca gcgttgctac caccagcgcc atcagcagac
     4681 caaacgcttt ttgcggcagc agatagttga ttaacaccac cagcgaagtg atcgctccgg
     4741 aaagcatcag cgagttaatc ggcacgccgc gacggctgac gcgagtcaaa aacttcggcg
     4801 cattaccctg cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat
     4861 acactgacag cgatgctacc agaatgacga agttcagcgc agaagctacc acgttgctgt
     4921 cgagattatg gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat
     4981 agagcgccag taaaactacc agtgaaccga tataaaacag caggatgcga tacactacct
     5041 gatttaccgc tttcggaatg cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa
     5101 tcagctccag accgccgaag gagaacataa tcaccgccag cgacaaaatc agcccattcc
     5161 agccggtggc aaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc
     5221 cgtgcccaga aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt
     5281 taatcagcgc aaaccagaat tcggtttcgc catataagcg cacgtttacc aggttaacgg
     5341 cgttgatgat aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt
     5401 actgcatata gatgcccgca gcggtcagct ctgccattcc caccagcacg aacatcaccc
     5461 agtagttcca gccggagagg aacccggcga acggtcccca gtatttatag gcaaagtggg
     5521 caaatgaacc ggataccggc tcttcgacca ccatctcgcc gagctggcgc ataatcagga
     5581 aagcgatgat cccggcgacg ccgtagccca gtaatacagc cggacccgcc atctgaatcg
     5641 ccgggccaat gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa
     5701 tatgacggtt atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg
     5761 atacggttga cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct
     5821 tttaacattt agtgcccatc gtcgcaagac acaatccaca cggttaaacc gggtatcctg
     5881 cttttatttc cgtacccgat gtcggtgtag ccacatcagc ttatacgccg ccgggataat
     5941 aaacagcgac agcaaaggcg cggtgatcat gccgccaatc atcggcgcgg caatccggct
     6001 catcacctct gatcctgccc ccgttcccca cagaatcggc agcagaccag cgataatcac
     6061 cgccaccgtc atcgctttcg ggcgcacgcg cagtaccgcg ccgtgataca acgcctcatc
     6121 cagcttctgc tcgctgaatg tttgcggatt attcaacgac ggctcggcct ctatggcgtg
     6181 acgtaaatac atcagcatca ccacgccaaa ttcggcggca accccggcaa gggcaataaa
     6241 accggtgccc gtcgccacag aaagatgaaa gcccatccac cacagcaacc agatgccgcc
     6301 caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaacgc
     6361 cagatacaac agcacgaaga tgatcatcaa cgtcatcggc accatgagtt tcagcttatg
     6421 gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa
     6481 ctgcactttt tcggcaatcg ctttttgcag atcgtgaacc accgacacca tgtcacgatc
     6541 gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg
     6601 tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt
     6661 caggatcggc aactggcgca gcgcctgcgg actatcgcgc cagctttgcg gataacgcag
     6721 attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc cgcccaccgc
     6781 agaagtcaca aacaattgca catccgccac cgtcataccg taacgcgcgg ctttttcacg
     6841 gttaatctca acgttgatat agcgcccacc ttccagacgc tcagcaagcg cagaagccac
     6901 gcctggcacc gttcgcgcca cttcttcaat ttgctccgcc atcgcgtcga tatccgccag
     6961 cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga gcatatcgat
     7021 acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca cggtgttatc
     7081 cagttcctca atgattttgt ccatcgtcat gcctggccgc cactgatcct gcggcttaag
     7141 ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc
     7201 ggttttgcca aatacccgcg ccacttcagg tacgctcata atcagcttgt cggttttttg
     7261 cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacaa
     7321 gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac
     7381 cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca gtactttcag
     7441 caacagcgga tgataaacac gaatcaaaaa gcgattgagc gggttactgc tttccggcgg
     7501 aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg ccagcagcgc
     7561 cgcacccgcc atcgcatacg ttttggtgaa cgccagcggg ccaaacagac gcccttcctg
     7621 cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaacag
     7681 cgccggcccc acttcaacag atgcatcggt gatcacctgc cagcgcgttt tattatccag
     7741 cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgtgcat tctcgatcat
     7801 gacgatagcg gcatcgacca tcgccccgac ggcaatcgca ataccgccca gcgacataat
     7861 attggcgttc agtccctgaa agtgcatgac aataaaagca atacacaacc ccagcggcaa
     7921 cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcac agactaccgc
     7981 caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag
     8041 ctggctgcga tcgtatgttg taactatctc cacgctttcc ggcagactgc ttttcagcgt
     8101 ttccagtttg tctttcacgg cggcgatcac ttctcgggcg tttttgccgg agcgcaggat
     8161 caccaccccg cccgccactt cgccttcgcc gttcagttcg gcaatgcccc ggcgcatctc
     8221 cgggccaatc tggaccttcg caacatcgcg cagataaacg ggcacgccat tttcactggc
     8281 ttttaaaacg atgtgattaa agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat
     8341 atattccgct tccgccagtt cgatcgacga accgcccgct tcctggtttg aagcatccag
     8401 cgcgcttttt acttcggcga gactgatgcc atattgcgcc aggcgctggg gatcgataac
     8461 cacctgatac tctttcacca caccgcccac cgacgccact tccgcaacgt cagggatggt
     8521 ttttagctca tatttgagaa accagtcctg caatgagcgt aaatcggcca gatcgtgctt
     8581 accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg catctggccc
     8641 cagctcagcg ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac
     8701 ccgcgagcgc gcccagtatg gatcggtgcc atcttcgaaa atgacataca cataagagtc
     8761 gccaaactgc gagaaaccgc gcacagtctt cgcgccaggc accgacaaca tagtggtggt
     8821 tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tgaccgggat agctggtttt
     8881 aataatcacc tgcacatcag agagatccgg cagtgcatcc actggcgtgt taatgatggt
     8941 ccaggtgccc cagatactca gaaacaacgc gcccatcagc accagaaaac ggttcgccac
     9001 cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg ggtagcactt
     9061 tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat caggaacagg
     9121 ccgctggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt gacgccttgc
     9181 gatgcctgga aaacggcaac gcgtttcggt acaaagcgcc cgtcggcatc aacggtaatc
     9241 acccgctgtt cgctgccggt atcaatcagc gcttgtgacg gaatgagcag catcggttcg
     9301 ctggcggtgt tgagttgcaa ccaggcgttc attcccggct ttagcgcctc gtcggtgttg
     9361 tcgacttcca ggcgcagctg cagcgtgcgg gttgcggcat ccacgccggg tagcagcgtc
     9421 catttgcgga tggtgagtgt tttatcaggt cgtgccggaa cggtgagcgt aaactgcgag
     9481 gcatctttca ccagccaggc gatggactcc gggatcgcgg cagtgatcca caccggatcc
     9541 ataccctgaa ttttcgcgac cacgttatct ttggcgatat tcattcccgc gcgcagatca
     9601 aacgcggtga tcacgccatc aatgggcgct ttgagcgtaa agcgagtctg gattttttgc
     9661 gtggcgatca ggcggcgaat atccgcctcc ggcattcccg ccagtctcag ccgctcaaga
     9721 atgccttcag tctgggtcgc cgtaccgcca gtttcgcgca gcagtaaata ctcactctgc
     9781 gcttccaccc agtcgggaat ggtcaggtcg agaagcggcg cgcccttttg tactttatcg
     9841 cccacggtaa gcggatacac cttgtcgata aacccggcgg cgcgggcctg cacaatggca
     9901 tactgatact cgttgtaact gacattcgcc gggaaacttt gggcaaaagt cagcggcccg
     9961 cgcgtgacgg tcgccgtttt cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca
    10021 ccagacgcag aactctcttc atcggcatat ttcggcacca gatccatatc cataaacggc
    10081 gatttacctg gtttatcgaa ccgcgtattg ggatacatcg ggtcgtacca gaataagatt
    10141 ttacgttctg cggtcgacgt tttttctgcg ggcggttccg cctttgcaaa ccaggtaaaa
    10201 cctgccgcag aaataatacc gcccgcgatc atgctgccga taataagcgc gatttttttc
    10261 atctcattaa acctgggtta ctggctgact ttaatatcct gtaataaaga aaggttgccc
    10321 tgctggacaa aattaaacgc cactttgtcg ccggttttta tttcgcccat cttcgtctgc
    10381 ggggtgatgg taaagcgcat ggtcatctcc ggccagttca cggcagcaat cggatcgtga
    10441 tggatagtga tttttttgct ttccagatca atacctttta ccacccctgt ggcactaatg
    10501 acctgcggtt gtgcttcgct catggtttca tgatgatgtt cgttagcctg ggcattaaag
    10561 ccaataacgg taaacagact gaacattgcg acttgcagtg cttttttcat ttattctctc
    10621 ctggagttaa aaattattgc tgccaaccgc caccaagtgc ggtatataaa gaaatttcgt
    10681 taacctgacg ggcataattc aggtcaagta aagtttgtcg ggttgcaaat aaagaacgtt
    10741 cggcatccag cacttccaga taactgactg cgccgtgctg atataatgcc cgcgcccgtt
    10801 gcaaagtaat ttgcagcgat gccagataac gctgctgggc gctaatttga tcgttcaggc
    10861 tttgacgtaa tgccagcgca tctgccactt ctttaaaggc attctggatt ttctgttcat
    10921 aattcaccac cgactgctgc tggcgaattt cggcgatatc cagattggcc tggttgcgtc
    10981 cggcattaaa aatggggatc tcaattttgg gaataaaatt ccacatcccg ctgctggcgt
    11041 taaataagga cgacagatcg ctgctggcgg tcgatattcc gctggtcaga ctgatagacg
    11101 ggaaaaatgc cgcgcgtgca gcgccaatat tggcattagc cgccatcaac gcgtgttcag
    11161 cttccataat atccgggcgc tgcaataaga tttgcgacga caagcccgcc ggtaatttaa
    11221 cgctttgcag gctgtcgctg tttactgttt gcgcttgcgg cagcttgccg tagcttccca
    11281 gcaacagttg caatgcatta ttcgcctgcg ccagttcccc ctgacgttta gcgatgtcgc
    11341 tgcgggtact ttctatcacc ccgcgagcct gttccagcgc cagaacattg ctgctaccgg
    11401 tcaacagttg tttttcgaca aacgcatatg actgctgata attacgcagc gtttcttcgg
    11461 ctatttgcaa ttgcgcatac gccagttgct gattgaaata gctttgcgcg acattagaaa
    11521 ccagcagaat atgcaccgcg cgctgggctt cctcagtggc taaataattt tggcgctcgg
    11581 cttcgctcat gttctttaag cgaccgaaaa aatcgagatc aaagctggcg ttaaggccgg
    11641 tcgaaaactc ccgtgtcgtg gcggtattcc ctttaagatt accgctccag ctgccgctgc
    11701 cctcgccatt gagctgtggg tagcggtcgg catcggtcag acgatattgc gcctgtgcct
    11761 cctgcacttt cagcgtcgcc atgcgcaaat cccggttatt caccagcgcc tcgctaatca
    11821 gcgtcttcac ctgattatca ataaaaaagg tgcgccagcc cgcgttctgg tagttatctg
    11881 ctgcgttaac caggccgttc tggctgagtg agaactgctt cggtacgggc attgccggac
    11941 gctgataatc cggtgccaat gaacaaccgg ttagcgcaag ggccacacaa aatggcagaa
    12001 gtttacaagg agacataggc acataatttc tggtgatttt atgccgccaa ctttacccgc
    12061 caggctctga ttttccggtg acaggaaaat gacaaaattg tcattttacc aataagcgat
    12121 tgccatctga tcccgctact ctagaattgc ccgggcaaca tgtggaggaa atatgaaact
    12181 gttgattgtc gaagatgaaa agaaaaccgg agaatacttg accaaagggt taaccgaagc
    12241 cggttttgtg gtcgatttgg ccgacaacgg gctgaatggc taccatctgg cgatgaccgg
    12301 agattatgat ctgataatcc tcgatattat gctgccggac gtgaacggct gggatatcgt
    12361 gcgcatgttg cgttccgcca ataaagggat gccgattctg ttgcttaccg cgcttggtac
    12421 cattgaacat cgcgtcaagg ggctggagtt gggggcggat gactatctgg tgaagccgtt
    12481 cgcttttgct gaactgctgg cgcgggtgcg cactctactg cggcgcgggg cggcggtaat
    12541 tatcgaaagt cagtttcagg ttgccgatct gatggtcgat ctcgtcagcc gcaaagtcac
    12601 ccgcagcggc acgcgcatca ctctgaccag taaagaattt actctgctgg agtttttcct
    12661 ccgccatcaa ggcgaagtgc tgccccgctc gcttatcgcc tcgcaggtgt gggacatgaa
    12721 tttcgacagc gataccaacg ccattgatgt ggcagtgaag cggctgcgcg gcaaaatcga
    12781 caacgacttt gagccgaagc tgattcaaac cgtgcgcggc gtgggttaca tgcttgaggt
    12841 gccggatggt cagtaagcca tttcagcgcc cgttttcgct ggcaacccgc ctgacctttt
    12901 ttatcagcct ggccaccatc gcggcgtttt tcgcctttgc ctggatcatg attcactcgg
    12961 taaaagtgca ttttgctgag caggatatta atgatttaaa agagattagc gccacactca
    13021 agcgcgttct taatcatccc gaagagacac aagcccgacg cttaatgacg ctggaagata
    13081 tcgtcagtgg ttattccaat gtgttgattt ccctggcaga tagccagggt aaaacggtgt
    13141 atcactcccc cggtgcgccg gatatccgcg agtttacgcg tgacgccata cccgataaag
    13201 acacgcgggg cggcgaggtg tatctccttt ccggcccgac gattatgatg ccgggccacg
    13261 gtcacgggca tatggaacac agcaactggc ggatgattaa cttgccggtt ggcccgttgg
    13321 tggacggcaa accgatttat acgctctaca tcgcgctttc gatcgatttt catcttcatt
    13381 acataaatga tttgatgaat aaacttatta tgaccgcatc gatcatcagc atcctgatcg
    13441 tctttatcgt gctgttggcg gtacataaag gtcacgcacc gatccgcagc gtcagccgtc
    13501 aaatccagaa cattacctcg aaagatctcg acgttcgcct tgacccgcag accgtgccga
    13561 ttgagctgga acagctggta ctgtcgttca accatatgat cgagcgtatt gaggatgtct
    13621 ttactcgcca gtccaatttc tcagcggata tcgctcacga aattcgtaca ccaattacga
    13681 atctcataac gcaaaccgaa atcgccctca gccagtcacg cagccagaag gagctggaag
    13741 atgtgctcta ctctaatctc gaagagctga cgcgaatggc gaaaatggtc agcgatatgc
    13801 tgtttctcgc ccaggccgat aacaaccagc taatccccga aaagaaaatg ctcaacctgg
    13861 cggatgaaat cggcaaagtg ttcgattttt tcgaggcgct agcggaagat cgcggcgtgg
    13921 aactgcggtt tgttggtgac gagtgtcagg ttgcgggcga tccgctgatg ctgcgtcggg
    13981 cgttaagcaa cctgctttcg aacgccctgc gttatacgcc gcccggagag gcgattgtag
    14041 tgcgctgcca gacggtcgat caccaggtgc aagtttccgt cgaaaacccc ggtacgccca
    14101 ttgcgcccga gcacttaccg cgattgtttg accgtttcta ccgcgttgac ccctcccgcc
    14161 agcgaaaagg cgaaggtagc ggtattgggc tggcgatagt gaaatcgatt gttgtcgcgc
    14221 ataaaggcac ggttgcagta acgtcagatg ctcgggggac aaggtttgtg attatgctgc
    14281 cggagagaga gtgattatta agttcaaatt cagggcgtgg tgcatcacct cctggtattg
    14341 ttgatcaaca aattagagaa aaacttatta aacatatgaa atatcgaagg cttttatttt
    14401 tcaaagtttc acgagatgtt tgtatatgaa acttagttaa atctgtgttt cttccaattg
    14461 tagatcacaa tgccctttct aaattttcat cgttataaac aagccatcta tcatataaag
    14521 caatgaataa tttaatcgcg attaatcatc attaattgtt atattattca ctcaatctat
    14581 ttatcaagtg aatttgataa attcctaatt taaaatatcc actattgagc cagtatttaa
    14641 caacgttaag
//
LOCUS       DARNVM010000120        14602 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.120, whole genome shotgun sequence.
ACCESSION   DARNVM010000120 DARNVM010000000
VERSION     DARNVM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.120"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            1..603
                     /gene="evgA"
                     /locus_tag="SI621_003338"
     CDS             1..603
                     /gene="evgA"
                     /locus_tag="SI621_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HEI4066010.1"
                     /translation="MIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDI
                     VIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGM
                     NNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDI
                     AEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            608..4201
                     /gene="evgS"
                     /locus_tag="SI621_003339"
     CDS             608..4201
                     /gene="evgS"
                     /locus_tag="SI621_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HEI4066011.1"
                     /translation="MKFLPYIILLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALTEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKDSVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     NIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAPIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINLSHQLEITPVSDDSKPEILKLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(4257..5402)
                     /gene="yfdE"
                     /locus_tag="SI621_003340"
     CDS             complement(4257..5402)
                     /gene="yfdE"
                     /locus_tag="SI621_003340"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HEI4066012.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNAHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5476..6420)
                     /gene="yfdV"
                     /locus_tag="SI621_003341"
     CDS             complement(5476..6420)
                     /gene="yfdV"
                     /locus_tag="SI621_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HEI4066013.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6489..8183)
                     /gene="oxc"
                     /locus_tag="SI621_003342"
     CDS             complement(6489..8183)
                     /gene="oxc"
                     /locus_tag="SI621_003342"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEI4066014.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNDIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLEIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8237..9487)
                     /gene="frc"
                     /locus_tag="SI621_003343"
     CDS             complement(8237..9487)
                     /gene="frc"
                     /locus_tag="SI621_003343"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEI4066015.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHSRQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(9999..10631)
                     /locus_tag="SI621_003344"
     CDS             complement(9999..10631)
                     /locus_tag="SI621_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HEI4066016.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            10927..11202
                     /locus_tag="SI621_003345"
     CDS             10927..11202
                     /locus_tag="SI621_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066017.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPPFPTKANYLIHPVR"
     gene            complement(11279..11521)
                     /locus_tag="SI621_003346"
     CDS             complement(11279..11521)
                     /locus_tag="SI621_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HEI4066018.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11874..12794
                     /gene="lpxP"
                     /locus_tag="SI621_003347"
     CDS             11874..12794
                     /gene="lpxP"
                     /locus_tag="SI621_003347"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HEI4066019.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13150..13221
                     /gene="ypdK"
                     /locus_tag="SI621_003348"
     CDS             13150..13221
                     /gene="ypdK"
                     /locus_tag="SI621_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HEI4066020.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13286..14524)
                     /gene="alaC"
                     /locus_tag="SI621_003349"
     CDS             complement(13286..14524)
                     /gene="alaC"
                     /locus_tag="SI621_003349"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEI4066021.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         4130 a         3421 c         3123 g         3928 t
ORIGIN      
        1 attattgatg accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat
       61 attgaaatct tagcggagtt gactgaaggg ggaagtgccg ttcagcgggt ggaaacactt
      121 aaacctgata tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta
      181 gaaacgctga ggaagcgcca atatagcgga attattatta tcgtctccgc taaaaatgac
      241 catttttacg ggaaacattg tgctgatgct ggcgctaatg gattcgtgag taaaaaagaa
      301 ggcatgaaca atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc
      361 ttctctctca accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta
      421 tcgaaacaag aaattagtgt catgcgatat attcttgatg gcaaggataa taatgacatt
      481 gcggaaaaaa tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa
      541 aaattagaat gtaaatcact gatggatctt tacacattcg cacaacgtaa caaaatcggc
      601 taaccacatg aagtttttac cctatattat tcttctctgt tgtggtcttt ggtcgaccat
      661 aagtttcgca gacgaagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac
      721 gcttgatcca ctacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct
      781 tgtgattgca gtacacaagt cccaaacggc tactttgttg cataccgatt cgcagcaacg
      841 ggttcgtggt attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt
      901 aacactccgg gaatacgcag atcatcaaaa agcaatggac gcgcttacag aaggggaagt
      961 cgatatagtg ttatcacatt tagttgcttc gccgcctctt aatgatgaca ttgctgcaac
     1021 caaaccgctg ataattacct ttccggcgct ggtcaccacc cttcacgatt caatgagacc
     1081 gcttacctca tcaaaaccag taaatattgc tcgggtagca aattaccctc cagatgaggt
     1141 aattcatcaa tcatttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt
     1201 agcatccgtc tcagccggac agaatgatta ctttattggt agtaacatta ttaccagcag
     1261 tatgatttcc cgctatttca ctcactcctt aaatgtagtg aaatattata actcgccgcg
     1321 tcaatataac tttttcttaa ccagaaaaga ttcagtcgtt cttaatgaag tactcaatcg
     1381 gtttgttgat gctttaacaa atgaagttcg ctatgaagta tcacaaaatt ggcttgatac
     1441 aggaaacctg gcctttctga acaaaccatt agaactcact gaacatgaaa aacagtggat
     1501 taagcagcat cccgatttaa aggtgctgga aaatccttac tcgccaccct attctatgac
     1561 agatgaaact ggctcagttc ggggcgtgat gggtgatatt cttaatatta ttaccttgca
     1621 aacaggttta aatttttctc cgatcaccgt ttcacacaat attcatgcgg ggacacagct
     1681 taaccccggt ggatgggata tattgcccgc cgctatttat agtgaagata gagaaaataa
     1741 tgttttattt gctgaagcct tcataacaac gccttacgtt tttgtcatgc aaaaagcgcc
     1801 tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc attccctatt attatgagct
     1861 tcattcgcaa ttaaaagaga tgtatccgga ggtagagtgg ataaaagtcg ataacgccag
     1921 cgctgcattt cacaaggtca aggaaggcga acttgatgct ctggtcgcga cacagttaaa
     1981 ttcacgttac atgatcgacc attactatcc taatgaactt tatcattttc ttattcccgg
     2041 cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat
     2101 tattaataaa gcactgaatg caattccccc aagcgaagtt ctgcgcctga cggaaaaatg
     2161 gattaaaatg cccaatgtga ccattgacac atgggacctc tatagcgagc aattttatat
     2221 tgtcacgaca ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt
     2281 acgctcagtt cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa tatcattccg
     2341 aaaagcactc tcggattcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt
     2401 cattagtcat aatagtgctt ttgaacatta tttcactgcg gattactaca aaaatgcaat
     2461 gttaccatta gaaaacagtg actcaccctt taaagatgtt ttttctaatg cgcatgaagt
     2521 cacagcagaa acgaaagaaa atcgaacaat atacacacag gtatttgaaa ttgataatgg
     2581 catcgagaaa agatgcatta atcactggca tacattatgc aatctgccag ccagcgatca
     2641 tgctgtttat atttgtggtt ggcaagatat taccgagacg cgtgatttaa ttcatgcact
     2701 cgaagtagag agaaataaag cgatcaatgc aactgtcgca aaaagccagt ttctggcaac
     2761 aatgagtcac gaaataagaa caccaataag ctccattatg ggcttcctgg aacttctgtc
     2821 gggttctggt cttagcaagg agcaacgggt ggaggcgatt tcacttgcct acgccaccgg
     2881 acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa
     2941 ctatcaactt caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc
     3001 tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt tgcagcagta cgtttcctga
     3061 acattacctg gttaagatcg accctcaggc gtttaagcag gtcttatcta atttactgag
     3121 caatgctctc aaatttacca ccgagggggc agtaaaaatt acgacctccc tggttaacat
     3181 tgatgacaac cacgctgtaa tcaaaatgac gattatggat tctggaagtg gattatcaca
     3241 ggaagaacaa caacaactgt ttaaacgcta tagccaaaca agtgcaggtc gtcagcaaac
     3301 aggttctggt ttaggcttaa tgatctgcaa agaattaatt aaaaacatgc agggtgattt
     3361 gtcattagaa agtcatccag gcataggaac aacatttacg atcacaatcc cggtagaaat
     3421 tacccaacaa gtggcggctg tcgaggcaaa agcagaacaa cccatcacac tacctgaaaa
     3481 gttgagcata ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct
     3541 aaatctatta ggatatgatg ttgatgaagc cactgatggt gtgcaagctc tacacaaagt
     3601 cagtatgcaa cattatgatc tgcttattac tgacgttaat atgccgaata tggatggttt
     3661 tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta cccatctggg ggcttacagc
     3721 caacgcacag gctaacgaac gtgaaaaagg gttaaattgc ggcatgaact tatgtttgtt
     3781 caaaccgttg accctggatg tactgaaaac acatttaagt cagttacacc aggttgcgcc
     3841 tattgcacct cagtatcgcc accttgatat cgaggccctg aaaaataata cggcgaacga
     3901 tctacaactg atgcaggaga ttctcatgac tttccagcat gaaacgcata aagatctacc
     3961 cgctgcgttt catgcgttag aagctggcga taacagaact ttccatcagt gtattcatcg
     4021 catccacggt gcggctaaca tcctgaattt gcaaaagttg attaatctta gccatcagtt
     4081 agaaataacg cccgtttcag atgacagcaa gcctgaaatt cttaagttgc tgaactctgt
     4141 taaagaacac attgcagagc tggaccagga gattgctgtt ttctgtcaac aaaatgacta
     4201 aatagcggct cccacaatat tcaaatgtgg gagctattta ccagcacatc ttacatttat
     4261 gatgagaact cctggcgaat ttgttcccca tgctggtcga gcgttgccgc tcccggcata
     4321 acatgcgggt ccgcgcagcc gctgattttt atcggattac ctggcatcat tattcccccg
     4381 gcttcaatca acatatttct cgcctgagtt tgtggcaaat taatggcctc agccacactt
     4441 aataacggcg cgacgggcac accaacttca tgtattttgg ctaaccaaac ttcagctgcc
     4501 tgcgttttta acgttcgctc aatatattgt ttaagaatag cctggttttg tacgcgtaaa
     4561 atattgctgc taaatcgggg atcattaacc agttccgtaa gctccagtgc ctggcataac
     4621 gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtatcaaaa
     4681 acatcaaaag gtgccatgta gggatggcga tttcccaggc gttgtggtga cttccctgtc
     4741 gcaatatatg ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata
     4801 tcgacatgcg cccctctctg gctcttttcg cggccataaa gtgcactcac tattccgctg
     4861 aataaataga caccgccgca tagatccgca agagaggtac caacacgcac tggaggagca
     4921 tcagggtacc ctgtttccat cattatcccg ctcattgcct gaataatggt atcgtaggca
     4981 ggagcatctt ttaacggacc ggtatgcccg aaacccgacg atgaagcata tatgaggcgc
     5041 gggttgattt cttgtaacct ttcccatgaa aaccccagtt tttccattgt acctgggcga
     5101 aaattctcag ctaatacatc agcttgttta agcatattta taaatatact tttatcgtga
     5161 gcattcttta aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt
     5221 gactgcccat ccacataggg accaaatgtg cgggtatcat caccatgacc cggcggctca
     5281 actttaatta cccttgcgcc catattacaa agaagttgag ttccgaaagg tccattaagg
     5341 acatgtgtca tatcgataac taataagcct tcaaacggcc ctttgcattc attatttgtc
     5401 atttgcttat gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat
     5461 caataacatg attaattatg aaaccagtcg actgacataa atccacaagg gagccgtgac
     5521 gacaaagccc agtacgctca cagccaatga tgctgtacca gtgcgggtgt agacattaaa
     5581 ccggctggca ataatgattc cggagaatgc cggcggtaag gcgcctgcca gtaccatcat
     5641 ctgcaaatgt tcgctgttca aatgacatgc cataccaacg agaagcagcg ccagtggcat
     5701 cagaatcagc ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc
     5761 agccagcgtc aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt
     5821 aaaggttggg tcccatgctg ccggaatttt taccccaacc aacaccagga tcgttgccag
     5881 aacaggtgcc catactactg gctcctttgc cgcagaaatt aatgcgctca gattactatt
     5941 cttcttacca tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat
     6001 tgcgttaaca ataatagaaa taattgctac aactaaacct gttgatacgg aatcaccata
     6061 aataggatcg agaactgcaa atcccaggaa tccgatggta ggtgagcctg caattaatgc
     6121 acatactgct gcttctgcat gggtacgttt aaaaaatttg tagcaaccaa accaggagaa
     6181 aaagaaacat ccgacaataa ccacaagtga tacaagggtc aggcgagtgt ccgcaaaaat
     6241 catttccctg tttgcccggg taatagaaac aaatagggcc gcaggaagag cgtagtttaa
     6301 taccagttta ttaaatgccc gagcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa
     6361 ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat
     6421 aatagtattt ccgataaggg tcccctcacc atcgataatg agttggcaag aggagacgat
     6481 gtcagtattt aattaccagc gacttgtttt gggttaagtt tggtaatatg gccactttca
     6541 gttcctgctg cagggtcgat gaccacatta ataatggtcg gtttgcgcga ctgaataccg
     6601 gtagttaaag catgacgaag ttcatctgtc gtggtgacgt tataaccaac gccacgaaac
     6661 gcatccatta atttgtcata ccttgcatgg tgcaatagat ctgttggtga tggtgcacca
     6721 gcgccactga gatcaacacc gtctcctctg tagatgccgc cattattaaa aataacgatc
     6781 gtcaccggca ggttatatcg acaaatcgtt tcaatctcca tcccactgaa accaaaagca
     6841 ctatcacctt caatggcgac aaccggagaa ccagaggtca cgctagcacc gatggcatag
     6901 cccataccga tgcccatgac accccaggtg ccacaatcca gacgacgacg tggtttatac
     6961 atatcaataa tatttcgtgc attatccagg gtatttgccc cttcattaac taaataaata
     7021 tcctggttct cacgcaatac atcgcgcaca gcactcaatg cattaaagta atttaatggt
     7081 tgggtatctg tacttaactt ttcatgcatt ttttgcgcat tttgctgctt gtggatattt
     7141 aaaatatcgc gccacaccag cggagtcgta aatgtgtttt gtttcaattc tgccagcata
     7201 ccttgcatac tggatgcaat atcgccaacg actggcacag caatgggacg gttgctgtca
     7261 atttcctgcg gttcaatttc cagttgaata aactgtgtat ctgccgccca tcctttttta
     7321 ccgtgcgcca gtaaccaatt cagtcgtgca ccaacaagca tgacaacgtc agcatttgcc
     7381 agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg tatcttccag gatccctttc
     7441 gccatagaca ttggcaggaa tggaatctgg gcactttcaa taaattcacg aagctgttca
     7501 tccgcttgtg aatacgccgc gcctttgcca aggataacta atggtcgttc agcttttgct
     7561 aaaagcgaaa ttgcgctagt gactgacttc gggcatggca ataatgctgg cgacggattt
     7621 tcaactttaa caatcgtggt taacgcttcg tctttttcca tcgtcgcggc caggacattt
     7681 gctggcaaat caagataaac tccgccaggg cgacccgata ctgagacccg gatagcgcgt
     7741 gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa acgccgcttt ggcatacggt
     7801 tttgccgcat tcatttggtc cagctcttca taatcacctt gctgtaggtc gacgatcgcg
     7861 cggtcgctgg agccgctaat catgatcatc ggaaaaccat ttaccgttgc gttggccaat
     7921 gcggtcaaac cattgaggaa tcctggcgca gacactgtca ggcagatccc cggtttttgg
     7981 gtaagaaaac cgcttgccgc agcggcatag cctgccgact gctcatgacg aaaaccaata
     8041 taacgaatgc cttccgcctg ggcatggcgc gccatatccg tcacaggaat acctacaaca
     8101 ccataaatag tgtcaatatc attctgtttt aatgcttcaa cgatgatatg cataccatct
     8161 gtcatttgaa gttgatctga catgattaat aacctcgaaa tttttataaa ttgggagcca
     8221 gaagcccccg ttaatatcag atggcgtggt tttgcttcat tgcagcaatt tcatcgtcgc
     8281 tataacccag ctcctgcaat acagcagcgg tatgttcacc taatagcggc gcagctttaa
     8341 tatccggcgt aaaggcagag aatttcattg gacaaccaac tgtcagatat tttccacgca
     8401 acggttgttc cacttcgacg acactgccac tttggcgcaa agagggatcg agtgaaattt
     8461 ctttcatact taaaaccggt gcacaaggaa tatcaaattg agtcaaatag gccaccgctt
     8521 catgtttatc aatagtgaca gtgtattttt cgatttcagc aaaaatatcg aaaatatgtg
     8581 gctgtcggga atgggctgta ctgtatgccg gatcggtaat ccaatctggt ttgccgatgg
     8641 ctttacaggt gttttcccag ttttgctcct gaatagtgaa ataaatatag gcgttaggat
     8701 cggtttccca gcctttacat ttcaggatcc agccaggctg accgcccccc cccgcattac
     8761 ctccgcgggg aactgcatca ccaaatgtgc cattcggata ctgcgggtat tcttccagat
     8821 aacccaattt atcgagacgc tgctggtcgc gtaatttcac gcggcaaagg ttcaatacgg
     8881 catcctgcat tgacatggtg actcgttgcc cacgccccgt tttttcgcga tgcagcaaag
     8941 cagcaagtaa accgatcagt aaatgcattc cggtgttgct gtcacccaac gctgcagcgc
     9001 ttaccagcgg cggaccgtcc caaaaacccg tagtggatgc agcgccaccc gctgcctgag
     9061 caacgttttc ataggctttt acattcacat aaggcgaaca ctcatcaaac cctttgatcg
     9121 aaccaaaaat cagacgtgga ttgatttctt gaatatgctc ccaggtgaag cccatgtgat
     9181 caatggcccc aggatgaaag ttctcgacta agatatcagc ttcgcggatc agcttttcca
     9241 ttacctcttt gccttccgct gttttggtat ttaattcaat agaacgtttg ttactgttaa
     9301 gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg cagctggtga cgcgttacgt
     9361 caccaacgcc gggacgttca attttaatga cgtcagcgcc aaaccaggcc agcatttgag
     9421 tacaagatgg gccagattgc acaccggtga aatcgagaac tttaattcct tgaagtggag
     9481 ttgacataga aatacctatt agttaattaa acatgtgtta atttagcgtt tatttaagac
     9541 ataacgcctc tataaataat ccgcaaaata tcgtcgtagc tgcactcgat atttttaaat
     9601 tcctgtatca agtactgaaa atcatttcat cgctacttcc aattcctttc tgcatggtaa
     9661 gtagtgagga cgaaaagaag aatatgataa atattgattc gtggtaatca gagagagccg
     9721 cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatgtac aagtaagggt
     9781 atgattacgc tttttaaagc gtaccataaa acataacata ataaatgatt tatgatgaca
     9841 cgggattatt gcggtttact ttgaagaata taaaatgcga ctttatagaa ccgcgagcgc
     9901 caaataattt tatggccaca acctgtcgta ttgtggccat aaaaaattgt cggctggagg
     9961 gagtaaaatc ctttacctca atatattgcc tgtcagcttt agttcacaaa cagtgcttcg
    10021 ctatcgacga tgattccatc ttcagcacct ttaagcgcca ggtttgcctc gtagtattgc
    10081 tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt gagcggcatc
    10141 agatattggt tttccattac gccaacaccg gccaggcgaa gctcttccat tgcgcccttt
    10201 ttatcccctt tcgccatttt ttcgttggca atttttatcg cggcttcttt ttcgggtgtc
    10261 gctacataac tctcagaaat tccgacagat gcattaatga caatatattg gtcatcattg
    10321 acattggtct ttttacctgg ctttgcgaac tttgcccatt cggtactatc atctgacagc
    10381 aaagcggaag cttcattggt cagttctttt gctttttccg gatcgccatg aaacagagcc
    10441 aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat acgctctgct
    10501 gctgccgttt tctgggtttg cttaacttgc tgagtttgct gttgtgccgc taccggtgca
    10561 ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt agccataatt
    10621 aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca tcatattggg
    10681 tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat cttacagttg
    10741 tagttattat cgcaagaagt attttagtct tacaattgta ggaatatgct tcctgtcaca
    10801 gagaactatt cttatatacc aaaaagagat gtcatttatt atatcgatgt aaatactgat
    10861 taattaaatg taaatacagc cataacatcg taggttctca gtgaaccatt ttcaggataa
    10921 ctttctatga aagtaaactt aatgcttttt agcttatttt tattggtctc tattatggca
    10981 tgcaatgttt ttgcattttc catttctggt ggtggaagtg agacgagcta taaagagaca
    11041 gaaaaaacgt cagtgatgac gacaactcat tctacaaaac ttcagccatc acaggcgatt
    11101 ttgtttaaga tgagagaaga tgcgccacca ttaaatctca cagaagacat cacgccccct
    11161 tttccgacaa aggcgaatta tcttattcat cctgtgcgat agcgtctgtc acatggcagg
    11221 tgaacaatca tatctgttga tgatgtgagc acctgcccaa atgatccatc ctgaactact
    11281 accgcctgcc ataccccacc ataaagcgaa tcatcaagcc acaagccggg gcaagaaaca
    11341 taaatatccc cgtcactagt tcattaccgc ctgttaacca catggtgaag tagtaaatga
    11401 gtgcagcgag gatcaccatg ccgaagaaag aagagaccgc cgagaccacg ttcagtacct
    11461 gaccgatgta tccgcttacg cagacaatac acagggcgag aaacatccat aaataaatca
    11521 tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa tagtaagaca
    11581 atccttaacc tctggcaatt tttgctaagc atcaatgaaa acagcatgtt aaatgcaaga
    11641 ctgttgcgta cggaaaaata tttactttgc acgattaata atcagtagct gaaagcagtc
    11701 agccagtgat aaagtacaga taccgcattc atgctcctcg tggttatgtc ctgactagtc
    11761 tttacactct ttacaggaac cattgtcgta catgatggcc caaccaattg agtgtaccgc
    11821 tgtcgacatg gaatcttctg cgatacaaca attcgtatct acagaaggta actatgtttc
    11881 cacaatgcaa attttcccgc gagtttctac atcctcgcta ctggctcaca tggtttgggc
    11941 ttggtgtact ctggctttgg gtacagcttc cttatcctgt tctctgcttt ctcggcacgc
    12001 gtattggcgc aatggcgcga ccattcctga aacggcgtga atctatcgcc cgtaaaaacc
    12061 tggaactttg cttcccgcag cattctgcgg aagaacgtga gaagatgatt gccgaaaact
    12121 ttcgttcact cggcatggcg ctggtagaaa ccggcatggc gtggttctgg cccgacagtc
    12181 gcgtacgtaa atggtttgat gttgaagggt tggataacct taaacgcgca caaatgcaaa
    12241 atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc ggccgcgtga
    12301 tgggactgtg ccaaccaatg atggctacct atcgtccaca taataatcag ctgatggaat
    12361 gggtgcagac ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga aataatctgc
    12421 gcggcattgt cggtgcactg aagaaaggtg aagcggtatg gtttgctccc gatcaggatt
    12481 atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc gccacaacca
    12541 atggtacgta tgtcctttca cgtctctctg gcgcagccat gttaaccgta acgatggtaa
    12601 gaaaagcgga ttacagcgga tatcgtttgt acatcacccc agagatggaa ggctacccgg
    12661 cagatgaaaa tcaagccgct gcctatatga acaagattat cgaaaaagag atcatgcgcg
    12721 caccggagca gtacctctgg atccaccgtc gctttaaaac gcgcccggtg ggagaatcgt
    12781 cgctatacat ttaacaaact gctcttactc tttccctcct caaatcagcc ataataacct
    12841 catggttttt atggcttttt tcttaaggtt ttttccttcg ctgaatctct aattattacc
    12901 gtcagaaatt aaactgtcgc cgatatgaga cacctgtaag ttactaaaag taaataagaa
    12961 aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctgga gcagtttagc
    13021 tcagaattct ggggtgatgc ccacggtaaa atacaggact tttaaggtcg ttttgcccgg
    13081 tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa ttacgcggag
    13141 agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt ttgggccggt
    13201 acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc catcaggctc
    13261 ctgtttgcat ttttgaggtc ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctgg
    13321 cgggtagtaa accatcggca cggaacatcg ctttaatccc acgaatcgcc tgacgaatac
    13381 ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtgtc gccgtagtcg ccaaagccaa
    13441 tccccggaga gacacagacc ttcgcttcgt tcagcagctt cttggcaaat tccagcgagc
    13501 ccatggcagc atacggttcc gggattttcg cccagacata catcgaagcc ttcggcattt
    13561 cgaccatcca gcccgcttca tgcagccctt ttaccagtac atcacggcgg cgtttgtact
    13621 gttcagcaat gtcgcgcacg cactgttgat cgccctccag cgccgcaatc gctgccacct
    13681 gcaacggcgt aaaggtgccg taatcgtgat agcttttaat acgtgccaac gcgctgacca
    13741 gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtaa cttttcgaca
    13801 gggtaaagaa ctcgaccgcc acgtcgcgtg cgcccggcac ctgcatgatt gacggcgctt
    13861 tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc
    13921 gttttgccag cgccacaacc ttctcaaaga attccagttc cacgcattgc gcggttgggt
    13981 tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcgcga atggcgcgtt
    14041 ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgcgccccgg
    14101 caatcaccgc gccgtaaata tgaatcgggt agcttggatt aggcactagt acagtgtcac
    14161 catgatccag cgtcgccagc atcagatgcg ccagtccctc tttcgaacca atggtgacga
    14221 tggcttctga ttccgggtcg atctcgacgt cgtagcgatc ctgataccag cgggaaatgg
    14281 cgcgacgtag ccgcggaatg ccgcgtgacg tggagtaacc atgcgtgtcc gggcgctggg
    14341 ccaccgtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg ttacccatac
    14401 tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtaatat
    14461 taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag
    14521 ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct gccacgatgg
    14581 tggctgtttt gaaaatagcc tg
//
LOCUS       DARNVM010000121        14477 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.121, whole genome shotgun sequence.
ACCESSION   DARNVM010000121 DARNVM010000000
VERSION     DARNVM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.121"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..880)
                     /locus_tag="SI621_003350"
     CDS             complement(<1..880)
                     /locus_tag="SI621_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4066022.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQ"
     gene            1037..1963
                     /locus_tag="SI621_003351"
     CDS             1037..1963
                     /locus_tag="SI621_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4066023.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            2163..3056
                     /locus_tag="SI621_003352"
     CDS             2163..3056
                     /locus_tag="SI621_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4066024.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLHVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3087..4076)
                     /locus_tag="SI621_003353"
     CDS             complement(3087..4076)
                     /locus_tag="SI621_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4066025.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFATQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(4103..4954)
                     /locus_tag="SI621_003354"
     CDS             complement(4103..4954)
                     /locus_tag="SI621_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4066026.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            5520..9770
                     /gene="fdeC"
                     /locus_tag="SI621_003355"
     CDS             5520..9770
                     /gene="fdeC"
                     /locus_tag="SI621_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HEI4066027.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVAADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLQKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKEQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTLTAIWSTKDANDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASQAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVCVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPANLALDPDGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(9895..10752)
                     /locus_tag="SI621_003356"
     CDS             complement(9895..10752)
                     /locus_tag="SI621_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066028.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            11000..11869
                     /locus_tag="SI621_003357"
     CDS             11000..11869
                     /locus_tag="SI621_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4066029.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAKAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(12029..12622)
                     /gene="rclC"
                     /locus_tag="SI621_003358"
     CDS             complement(12029..12622)
                     /gene="rclC"
                     /locus_tag="SI621_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HEI4066030.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(12634..12870)
                     /locus_tag="SI621_003359"
     CDS             complement(12634..12870)
                     /locus_tag="SI621_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4066031.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(12979..14304)
                     /locus_tag="SI621_003360"
     CDS             complement(12979..14304)
                     /locus_tag="SI621_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEI4066032.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSIGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3876 a         3267 c         3530 g         3804 t
ORIGIN      
        1 cctgtttcac aggttctggc ttatcataca ggtcgtaatg tgaataacct tcaaccacga
       61 ccagctcttt atgttttgag gcagcgcggc caataatttc gcagccgtca cggtaggcac
      121 caaatgcgcc cactctatcg cccaccacaa ccattattgg ttgagttaac aggacttctg
      181 caagatgaaa tgcatcccaa cccacggcaa cagtctgatg tgagaatagc gaacgattta
      241 ccccattagg tgcacagcct cgtggcgaac gataatattc tgtggcttca aaaaggtcta
      301 tttctgttaa tcccgcttca agagctgcat ccggagaaga ggggagtaaa tcatctacgc
      361 gaagttttgc gccgttagct tcgtctgttc tttgttgcgc catcgcttcg agtgctccga
      421 taggattaaa cgctgtaaat ccctcacgca taagacgccc gtagtttgca cccgtgacag
      481 taccaatagc tttaattcga cgttctgtca ttgcggcatt aatggcatat ccacctccac
      541 cacaaatacc cagtacacca atacgcccgg catcaacgta aggtaaggtg gtaaggtaat
      601 caacgacaat gctgaagtct tttactcgca ttgttggatc ttccagataa cgcggttctc
      661 caccgctgct tccctgaaaa ctggcatcaa aagcaatgac gataaaacca gcttttgcca
      721 gggcggcacc atacacacta cccgatgtct gttctttgca gcttccaatt ggatgggcgc
      781 tgataatggc aggatacttt ttttcactat taaaatcgtc agggaaatag atatcagcag
      841 ctatatccca gtaagtatgt tttatcgaaa cagttttcat ttcaagctcc ggggatttgc
      901 ctaaacagat ttaaatatca tttctattat ccgcaactgg cattcgtgtt catatcgaga
      961 acggcgtaat agtatgactt gtattggtta tcatcaatgg tctaatatct atcacagtgt
     1021 taagttaagg tttacaatga tgaaaataga gccttcaatt ttgccttctc ttgcctggtt
     1081 tgcgcttatt gttcgtgccg gcagtttttc cagagccgct tctgaaatgg gtattacgcg
     1141 ggccgcattg tcccagaatt taaaatctct tgaggaacgt cttaacacca agctgatata
     1201 ccgtacgacc cgcaatatgt cgcttactga ggaaggacag cgtctgtatg aagtgctggt
     1261 gtcggctttg gggcaaattg atgatgcttt gaaagatgtt ggtgataccc aacttgaacc
     1321 tacaggcctg ttgagaataa attcctcccg tgttgcagca cggatgcttg ttgagcctca
     1381 tattggcgag tttcttactc gttaccccaa aacaaagata gaactcatta tggatgacgg
     1441 tttatctaac attattgcgg acggctgtga tgtgggcata cgtcttgagc aaggactgga
     1501 tgagcatatg actgccgttc ctgtttctcc gcttattaaa ctggttactg tggcatcgcc
     1561 agattattta aaagaacacg gtattcctga aacgcctcag gaactgagta accataattg
     1621 ccttcggttg cggcataaaa gtagcggtgc actctctgca tgggagtttt ctaatgttgt
     1681 ggggggtaat gaagagttcg aaatagaggt ttcaggtaag tatatttcta atgatgacga
     1741 aagcatgata cgaatggcgc tgaatggaac gggtatcatt caacatctgg attttgcaat
     1801 cgctgaacat atcaatgcgg gcaagttaca gccgattctt gaggattggg ccgtttcatt
     1861 tcctggattt tatatatatg tttcatcacg ggtgagaatg ccctctaaag ttcgtgcttt
     1921 tattgatttt atggtggaga aaagagtaaa aatagaaagt taatgatgtg tgtcagaata
     1981 tcggtaagaa gaatggcgca atcgtgagga tacgtttatt cataataaat ctcctgaaag
     2041 tttagaatta ttttcgatac agccagatta tcattgttac taataatgtg gttaaaataa
     2101 tagcatctat gccccccttc atgagtgtct gtgaagagga tgcttctaca aggaaatcgc
     2161 caatgctcaa agaaaacttc aacgaactgc aaatctttct tgtggtggca agagagcgaa
     2221 gttttaccaa agcagcgggc aaactcggcg tctctcagtc tgcacttagc catgcaatga
     2281 aggctctgga ggaaaggctc aatatccgcc tcctgacacg gactacccgc agtgtggctc
     2341 ccacagaagc gggggagaga atcattgcct gccttgaacc gcgtattgac gagcttgaac
     2401 aggaactgga gtcacttatt caacagaacg gcacgccctc cgggaatatt cggttatctg
     2461 ccggggaaca tgccgcgcga agtctggtct ggccgaagct aaaacctttt ctcagagagt
     2521 atcccgaaat caatgtagaa ctggtggtgg ataacggttt tgtcgatatt gttgaaggcc
     2581 gttttgatgc cgggatacga ctgggtgaaa atgttgataa agatatggtt gctgtcagga
     2641 ttggaccaga tatgcggatg gctgtggtgg gagctccggc gtattttgct gcaaaccctg
     2701 cgcctgaaac gcctcacgag ctacaaaatc atcgctgcat caacatgcgt ttgccaactg
     2761 ccggtgggct ttaccactgg gagttcgagc gagaagggaa accgcttcac gttaaggttg
     2821 atgggcagct gacgtgtagt ccgttgccag aaagaattga tgccgcctta tcagggtttg
     2881 ggatcgcttg tgtccctgaa gacatggttc aggagtatat aaagtcaggc aagcttattc
     2941 aggtgttaca ggagtggtgt ccaacttttc ctgggtatta tctttactac cccagccgca
     3001 agcagcatcc gccagctttt gcgctgttga tcgatgcgct tcgctacacc gaataacggg
     3061 tcctttatcc tggacccgtt tgggtattag cggcctacgc gtgcctgatg ttcaggcgag
     3121 taacgttcac cgacgatttt aattgtttcc agcgcctcgg ttatctgctg cgtatctttc
     3181 tgcgaaagaa caatgtcagc agcggccagg ttttcttcca gccggttcag tttagtggtg
     3241 ccagggatgg gaacaatcca cggcttttgt gctaatagcc atgccagtgc gatttgtgca
     3301 gacgttaccc ctttctccgc agccagttca cctaacaagg tgaccagttt ttcattggct
     3361 tcaatggctt gtgttgcgaa acgcggtacg gtgctgcggt aatcatcttt gccgaaagtg
     3421 gttcccggct taatcgcccc tgtcaggaaa cctttaccta gtgggctgaa ggggacaaaa
     3481 ccaattccca gttcctccag caacggcaga atttcctgtt caggctcacg ccaccacatg
     3541 gaatattcgc tttgcagggc ggtgacaggt tgtaccgcat gtgcacgacg aatggtttgt
     3601 gctcctgctt cagacaggcc gaaatgtttg accttacctt ctgcgatgag gtctttcacc
     3661 gttcctgcaa catcttcaat aggaacatcc ggatcaacac ggtgttgata cagcaggtca
     3721 ataacatctg ttttcagacg acgtaatgat ccttcaacgg cttcacggat atgttccgga
     3781 cggctgttta aaatctgctg cttattatca tcgccgaagg taaaaccgaa tttagtggca
     3841 attaccacgc ggtcacgaaa cggttttaag gcttcgccga ccacttcttc attcagatac
     3901 ggaccataca cttcggcggt atcgaagaag gtgacaccac gttcaaccgc agcacgaata
     3961 agttcgatag cctgacgggt atcggtcgcc gggccgtagc cgtggcttaa gcccatgcaa
     4021 ccgagcccaa gggcggagac ttcgagtccg gatttaccca gataacgttt ttgcattgaa
     4081 tgaatacctc ttttatcgcg gcttaaacat caagtttgcg gccagtcagc cattccacca
     4141 ttgcgggatc gcggtgagag aagaacgcgc tggttgcggt atccagggcg gtaatttgca
     4201 gcatatcttc agggctgagt tcaaaatcga gaatgttgat gttctcttcc atgcgttctt
     4261 ttcgcactga ttttgccagt gaaacaatgc ctcgttggta gatccagcgc agaacaacct
     4321 ggcccacgct tttgccatac ttctcgccaa ttgccgtgag aacaggatgc tggaacagac
     4381 catttttccc ttccgcaaac ggtgcccagg cttctggctg aatgccacga ctttgcatcc
     4441 agggaacggc ctgtagctgc tgattgaaag ggttaacttc aacctggttt accgccggaa
     4501 cgacgttatt aaaggcgata aggtcagcca gtcggtcagg atggaagtta ctgacgccaa
     4561 tagcgcgaat tttaccggcc tgttgcagct cttccatggc acgccaggct ccgtggacat
     4621 cgccatatgg ctggtgaatc aagtacaggt caacgtaatc aagttgcagt cgattcagag
     4681 agcgttcgaa ctgggctttg gccccttcgt aactggtatc ctgcaaccag agtttcgtgg
     4741 tgacaaagag ttcgttacgg gcgataccgc tttgtttcag tgcgtttccg acctggattt
     4801 cattttgata agaggcggcg gtgtcaatca gtcgataacc gctgttaata gcgtcaatga
     4861 ccgctctttc gcattcggcg gcatcggtca tctgaaatac accaaagccc aacaggggca
     4921 tttcaatacc gttgttcagt tttacttttt gcatggtgtt atccttccgt tatcgttcat
     4981 gagaagcata acgtaaaggg aaaagctcga ttagacggca ggatttgtca ggggttatga
     5041 acaaaattca taaatctgtt tgagggttga gattagtcgt gcaagttcga tatccccgga
     5101 atttacagtc cgatgaatat aaatgaatgt ccataaaaaa tatattggtt tatcctggcg
     5161 tatatactca cttcgacgta ctcccaatag cagttaatta aagacgggtc attattattc
     5221 actcttaata atgaattatt cactgcccgc agagtaagaa atataactta agtatctatt
     5281 taataacttg cacaaaaagc taaattttta cccataaata aaaatataat cccgcgccca
     5341 accacctgat gagtggctac agacactgga tatattaggt ggcggtgtac tttcttacat
     5401 aaaggtattt ccttttctgc ggaaaaggaa accgggaaat ccccggtttt tctgacaagc
     5461 agacgccatt atttgtgtct gcctatgttc gttaattcgt tcatcaggaa attatctcaa
     5521 tgtcacgtta taaaacaggt cataaacaac cacgatttcg ttattcagtt ctggcccgct
     5581 gcgtggcgtg ggcaaatatc tctgttcagg ttctttttcc actcgctgtc acctttacgc
     5641 cagtaatggc agcacgtgcg cagcatgcgg ttcagccacg tttgagcatg gaaaatacca
     5701 cagtagctgc tgataataat gtggagaaaa atgtcgcgtc gtttgccgca aatgccggga
     5761 catttttaag cagtcagcca gatagcgatg cgacacgtaa ctttattacc ggaatggcca
     5821 ccgctaaagc taaccaggaa attcaggagt ggctcgggaa atacggtact gcgcgcgtca
     5881 aactgaatgt cgataaagat ttctcgctga aggattcttc gctggaaatg ctttatccga
     5941 tttatgatac accgacaaat atgttgttca ctcagggggc aatacatcgt accgacgatc
     6001 gtactcagtc aaatattggt tttggctggc gtcatttttc aggaaatgac tggatggcgg
     6061 gggtgaacac ctttatcgac catgatttat cccgtagtca tacccgcatt ggtgttggtg
     6121 cggaatactg gcgtgattat ttgaaattga gcgccaatgg ttatatccgg gcttctggct
     6181 ggaaaaaatc gccggatgtt gaggattatc aggaacgccc ggcgaatggc tgggatattc
     6241 gtgctgaggg ctatttaccc gcctggccgc agcttggcgc aagcctgatg tatgagcagt
     6301 attatggcga tgaagtcggg ctgtttggca aagataaacg ccagaaagat ccacatgcga
     6361 ttaccgctga agtgaattac acgccagtgc ctcttctgac cctgagtgcc gggcataagc
     6421 agggcaagag tggtgagaat gacactcgct ttggcttgga agttaattat cggattggcg
     6481 aacctctgca aaaacaactc gatacagaca gcattcgcga gcgtcgaatg ctggcaggca
     6541 gccgctatga cctggttgag cgtaataaca acatcgttct tgagtaccgc aaatctgaag
     6601 tgatccgtat tgctctgcct gagcgtattg aaggtaaggg cggtcagacg gtttccctgg
     6661 ggcttgtggt cagcaaagca acacacggac tgaaaaatgt gcagtgggaa gcgccgtcat
     6721 tactggctga aggtggcaaa attaccggtc agggtagtca gtggcaagta acgctcccgg
     6781 cttatcgtcc aggcaaagac aattattatg cgatttcagc agttgcctac gataacaaag
     6841 gcaatgcctc gaaacgtgtg cagacagaag tggttattac cggagctggt atgagcgccg
     6901 atcgcacggc gttaacgctt gacggtcaga gccgtattca aatgcttgct aacggtaatg
     6961 agcaaaagcc gctggtgctg tctctgcgcg acgccgaggg tcagccagtc acgggcatga
     7021 aggagcagat caagactgaa ctaaccttca aaccggctgg aaatattgtg actcgtaccc
     7081 tgaaggccac taaatcacag gcacagccca cactgggtga gttcaccgaa actgaagcag
     7141 gggtgtacca gtctgtcttt acgaccggaa cgcagtcagg tgaggcaacg attactgtta
     7201 gcgttgatgg catgagcaaa accgtcactg cagaactgcg ggccacgatg atggatgtgg
     7261 caaactccac cctgagcgct aacgagccgt caggtgacgt ggttgctgat ggtcagcaag
     7321 cctacacgct gacgctgact gcggtggact ccgagggtaa tccggtgacg ggagaagcca
     7381 gccgcctgcg acttgttccg caagacacta atggtgttac cgttggtgcc atttcggaaa
     7441 taaaaccagg ggtttacagc gccacggttt cttcgacccg tgccggaaac gttgttgtgc
     7501 gcgccttcag cgagcagtat cagctgggca cattacaaca aacgctgaag tttgttgccg
     7561 ggccgcttga tgcagcacat tcgtccatca ccctgaatcc tgataaaccg gtggtcggcg
     7621 gtacgcttac ggcaatctgg tcgacaaaag atgccaatga caaccctgtg accagcctca
     7681 cgccggaagc gccgtcatta gcgggtgccg ctgctgttgg ttctacggca tctggctgga
     7741 caaataatgg tgatgggacg tggactgcgc agattactct cggctctacg gcgggtgaat
     7801 tagaagttat gccgaagcta aatggacagg atgcggcagc aaatgcggca aaagtaaccg
     7861 tggtggctga tgcgttatct tcaaaccagt cgaaagtctc tgtcgcggaa gatcacgtaa
     7921 aagccggcga aagcacaacc gtgacgttgg tggcgaaaga tgcacatggc aacgctatca
     7981 gtggtctttc gttgtcggca agtttgacgg ggaccgcctc tgaaggggcg accgtttcca
     8041 gttggaccga aaaaggtgac ggttcctatg ttgctacgtt aactacaggc ggaaagacgg
     8101 gcgagcttcg tgtcatgccg ctcttcaacg gccagccagc agccaccgaa gccgcgcagc
     8161 tgactgttat tgccggagag atgtcatcag cgaattctac gcttgttgcg gacaataaaa
     8221 ctccaacggt taaaacgacg acggaactca ccttcaccgt gaaggatgcg tacgggaatc
     8281 cggtcaccgg gctgaagcca gatgcaccag tgtttagcgg tgccgccagc acggggagtg
     8341 agcgtccttc agcaggaaac tggacagaga aaggtaatgg ggtctacgtg tcgaccttaa
     8401 cgctgggatc tgccgcgggt cagttgtctg tgatgccgcg agtgaacggc caaaatgctg
     8461 ttgctcagcc actggtgctg aacgttgcag gggacgcttc tcaggctgag attcgtgata
     8521 tgacagtgaa ggttaataac caactggcta atggacagtc tgctaaccag ataaccctga
     8581 ccgtcgtgga cagctatggt aacccgttgc aggggcaaga agttacgctg actttaccgc
     8641 agggtgtgac tagcaagacg gggaatacag taacaaccaa tgcggcaggt aaagcggata
     8701 ttgagttgat atcaaccgtt gcaggggaac ttgaaatcgc ggccgcggtg aaaaactctc
     8761 agaagacggt cacggtgaaa ttcaacgcgg atgccagcac cggtcaggca aacctgcagg
     8821 ttgacaccgc cgttcaaaaa gtggcaaacg gcaaagatgc ctttacgctg acggcgaccg
     8881 ttgaggataa aaacggtaac cctgttccag gcactctggt gacctttaat ctgccccggg
     8941 gtgtcaagcc gcttactggc gataatgtct gtgtgaaagc caacgatgag gggaaagcag
     9001 agttgcaggt ggtttccgtg actgccggaa cctatgagat cacggcatcg gcaggaaatg
     9061 accagccttc ggatgcgcag actataacgt ttgtggccga taaggcgacc gcaaccgtct
     9121 ccggtattga ggtgattggc aactatgcac tggcggatgg caaagccaaa cagacgtata
     9181 aagttacggt gactgatgcc aataacaacc tgttgaaaga tagcgaggtg acgctgactg
     9241 ctagcccggc aaatttagct ctggatcccg atgggacggc gacaacgaat gagcaaggac
     9301 aggctatttt caccgccacg accactgtcg cagcgacata tacactcacg gcgaaagtgg
     9361 aacaggccga cggtcaggaa tcgacgaaaa ctgccgaatc taaattcgtc gcggatgata
     9421 aaaacgcgga gctcgctgca tctccagagc gtgtagattc tctggtggcg gacgggaaga
     9481 ctactgcaac actgacggtt actctgatgt cgggtgtcaa ccccgtagga ggaaccatgt
     9541 gggtcgacat tgaggctccg gaaggggtga cagaggcgga ttatcagttc ctgccgtcga
     9601 aaaatgacca tttcgcgagc gggaaaatca cgcgtacatt tagtaccaac aagccaggta
     9661 catacacatt cacattcaac tctttgacat atggagggta tgaaatgaaa ccagtgactg
     9721 tgacaattaa cgccgttcct gcagatactg aaggtgctga ggagaaataa aaacgacgat
     9781 gtaataaata ataatggcgt tgacatgcgc agcgccatta tttactgaat acctaccctg
     9841 gtaccgatta gacggtggca gggtagggta aatcttatgt gatggattta caggctaaac
     9901 cgggcgaacg gggataaaaa tttcgcaaac aatgttattg ctatcaattt cgacgaagtt
     9961 ttcattgtaa taaaatcgct caatatcact gccgttcact ctggctaacc ccctggcagg
    10021 taattccatc agatagaggt ttcgcgccca ttcagggtag tgctcccact caccttcata
    10081 gcggaaagag gcatacagtc ctcggttaat tgtccactct ttcgacatct ctgaatcggt
    10141 ttgtttgcat accgtaccgg caaaagtttc aactgtgagc aaatcgaccg attttgttga
    10201 cggttcgaat gtggagacga aatagatatc cgtcttaaaa ttcttcatac atctggctaa
    10261 atgatgacgt cgcagtttga cttcttcatc cggtgattta ttcgatactt caatatcata
    10321 tttaaatgaa ttgccaaaaa taggtgtctc aggaaagttg actaatctac actccgtctt
    10381 ttgctgttga cgtattaaaa aagaagggaa gatatttgcg agatcccaat aatcacggtg
    10441 gcggtattca cggggagagc agccgaataa ctttttaaat tcgcggctga atgactgctg
    10501 tgagtcaaaa tgtaaagaga gtgcaatatc aagcatagat ttcgcggtga gtcggacaag
    10561 aatggcggca cgacaaagcc ttcgtttgcg aatgtattct cccaaaggca catgcatgaa
    10621 atttcggaac agaagctgga tgttgcgtct gctgtagcca gatttctgtg cgatatcttc
    10681 gattgaaata gggtgctcaa gattgcactc aatccactcc aggagctgct gtagaatctt
    10741 ctgcctgatc atcacattct ccgttaattc tttgtcatat cgcacatttt cgttatgtcc
    10801 tgggccttaa gtgtatcaaa aacagcaagt cccaaggcaa aagcactgat agcgtctgct
    10861 tcattgcccc aaaaatagag aggtttcagt cctacattat taatgaattt tttgcataag
    10921 tgatatcaaa atccacatac taatttgagg ttacgtttta acgtagactc attgttcata
    10981 cctaatggag ggactgacag tggaattttc cgtattaagt aacaatctga aaatgccgat
    11041 gatgggattt ggtgtttttc aggttaccga taaagacgtg tgcaaacagt cagtgttgaa
    11101 tgctatccgc acgggttatc gactcatcga cactgcagcg gtatacggta atgaagatgc
    11161 cgtcggtgaa gccgttcgtg aagctatatc tgaaggttta tgtacccgag atgagttatt
    11221 tataacgtct aaattgtggg tgcaggatat gttgaatcag gatacggcaa aagcaggtat
    11281 tgaagcatca ttaaaaaaat cgggactaga atacttcgac ctctatttat tgcaccaggc
    11341 tatgcgcgat tatttcagtg cgtggcgtgc actcgaagat gcctatgaag agggcaaatt
    11401 aaaagcaatt ggggtttcca atttctatcc tcatgttctg gcgaactttt gtgaaacggt
    11461 aagagttaaa ccgatggtca accaggtcga gttgcatccc tattttgccc aaccagaggc
    11521 gctggcaacc atgaagtatt ataacgtgca gcctgaagca tgggctccgt taggtggtgg
    11581 acgacataaa ccctttgaaa ataatctgct tcagagtatt gcagatgccc atcaaaaatc
    11641 gatttctcaa gtcattctgc gttggaatat tcaacgggga gtggtcgtta ttccgaaatc
    11701 gacacatcaa cagcgtatcg aagaaaattt tgctatctgg gatttctcac tgacagagaa
    11761 agaaatggca caaattagtt cgcttgattt gggttatgtt ggggagtcgg taaaacattt
    11821 taatcctgaa tttgttcgtg gttgtcttgc tgtaaaaata catgattgat attaatcatg
    11881 tattttacct gagacgacaa gaatctttta acaggggagt gatattgatc ttcactctgt
    11941 catatctccg gtaatatggc gtcaggcttc atcatcagaa agggggcctg acgcctgaaa
    12001 aagtgaacaa cagacagtgt tcggattatc aatattcagt ttttaacgtt gaactggatt
    12061 cattactgcg ttgtttaaga atttcccgcg ccgaatctgc cattatcatg acagcacctg
    12121 ccagcatcag agtatctttc aataccaggc gaccagcacc agataaataa gggaaaccat
    12181 gatgagcgtc acccaatgcg ggtacccatg cctccggggt ggtgattaaa aatgagagtg
    12241 ttaccaacgg tgtggtaaat gccatcagcc ctcccaataa acctaaccag cgattgacag
    12301 gattagccaa aaccagcaga gcaataatca cctccacgac gccaagaccg ttggaaaaac
    12361 cataggtatt attggccgat tgccatgccc ttgcttctgg tttgtattcg ccttcgtgag
    12421 tcagatactg tttatagtct tccgggtgtt cataaaagaa cgacattagt ggactgtttg
    12481 cgacgaatgg tgtaatgctg tctgcctcgt aagggacaaa ctttaataac ccaatccaca
    12541 taaaaacaat cgcaatactc agacgaatca atgtcaggcc aattttatct ccccgactta
    12601 acaggtgaag gtatttttcc attgtgaaca tccttattta tacaatactg ctgttgcggt
    12661 tgaattatta tcaattcgca tcgatgtgac tttccaggat gaagcattat attttctggc
    12721 tatatcagca atggcatcct ccagaactgt cggtgatgct gatactgacg agacgctgat
    12781 atgtttcaga attattttat catctgcatt ggtggaaaat gccataacgc cagataatag
    12841 tgttgcaaat aaaacagatt ttttaaacat ataaaatacc atcaatgaag tgattaagaa
    12901 gttatagagt aacagagaat taataagatt cttttctctg agacgccaga atatttgttc
    12961 tggcgtctga ttttgagttt atttgactaa tgaaaataga tcattgagtg attcgctcat
    13021 cgacggatga gtaaatatct gatcgcgtaa tatgctataa ggcagcccgg catccatcac
    13081 cattttcact atattaatca tctcgtggga gtcaacacac agcagtgatg cccctaatat
    13141 acgttgggtt ttattatcaa caatcgcttt taatacccca cgggtatcgt tcatcactct
    13201 ggcgcgcgga attgcagcta caggcaatgt caccacctga atatcagcac cactctctct
    13261 ggcttgttct tctgtcatac caaccctgga caggggcggt gtcataaata cggaataagg
    13321 cacatttttc cgatcatcag tactacgttt gccttcaccc agtaactcat cacgtacaat
    13381 gcggtaatca tccagtgaaa tataagtaaa ttgcagcccg ccggtaacat ctcccatcgc
    13441 ccaaatattg tctgcggtgg tatgtaatcg cttgtcaacg acaattgccc cgcgctcgtt
    13501 tactgcgata ccggcatttt ctggatgtaa cgaagcggta gccggttgac gaccggaagc
    13561 tattaacagt gcatccaccg ccagttgggc gtgctcgcta tgcacttgca cttgattttc
    13621 atggtgactg attcgctcca catgggcatt gaggataata tcgacgccct gatcgcgtaa
    13681 aatcgtcgcg atattatcag caatatcccg atcttcccga ggcaaaaaca gcgaagctgc
    13741 ttctaaaatg gttaccttgc tgccaaaatt agcgaacata gaggcgaact caacgccaat
    13801 atatccgccg cccaaaatac ctaaatgccc aggcaattct tttagattaa gtaatccgat
    13861 gctgtcatat actcctggcg tggtggtaat tccaggaatt ggcggaacca cggtttgtgc
    13921 accggtatta ataaaaattt tctcgccatg aatttccaga tttccctcag gccgatgaac
    13981 acgcaggcta tgattattga taaactccgc ctggccgtcg atcacgtcga tattgggcat
    14041 atccgcaaga ttatgaaaat tcttattacg taaaaaatta accacttcat ttttacgctg
    14101 tatggcacgg acaaaatctg tgtgctgctg tgcgtcatga accaatgttt tggttgggat
    14161 acagccgata ttaatacagg tcccgccata cattgcattt gattgttcga tgagagccac
    14221 acgccaacct gcttttgcca gcgtgacggc taatgttttt ccagccttgc caaaaccaat
    14281 aataactgcc tgatatttat tcatgatgat ttcctgtcga aacgcgttca tttgacatca
    14341 tgactataga cgcaaaagag caaataacat ttcttcacaa cgcttgatgt tttgtcttat
    14401 cgtctggtat tttctttttc ttaatcagaa tcacaaaaca aaaatcatga ttaactattt
    14461 gatggtatta gattaat
//
LOCUS       DARNVM010000122        14458 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.122, whole genome shotgun sequence.
ACCESSION   DARNVM010000122 DARNVM010000000
VERSION     DARNVM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.122"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            53..1321
                     /locus_tag="SI621_003361"
     CDS             53..1321
                     /locus_tag="SI621_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEI4066033.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEDHANRVKKSLEDNIFPAIGSRNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     SKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANRERAITPFDYAKINRGNGE"
     gene            1537..1653
                     /locus_tag="SI621_003362"
                     /pseudo
     CDS             1537..1653
                     /locus_tag="SI621_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1660..2240
                     /locus_tag="SI621_003363"
                     /pseudo
     CDS             1660..2240
                     /locus_tag="SI621_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2311..5349)
                     /locus_tag="SI621_003364"
     CDS             complement(2311..5349)
                     /locus_tag="SI621_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEI4066034.1"
                     /translation="MSMKIQFDANLDYQRDAIESVTGVFAGQEICQTNFTVAPLHSWE
                     EGSLFHGIQEDTLGIGNRLRLLDEDIHANIRKIQLKNGLAPSESFDSKYGIHLTVEME
                     TGTGKTYVYLRTVFEMNRLYGFTKFIIVVPSIAIKEGVQKSLEITATHFKELYENVAF
                     DYFTYDSSKLSDVRNFATSPNIQIMVINIDAFRKSFSDPEQENKANIIHRAHDRMTGA
                     KPIEFIQQTNPIVIVDEPQSVDTTDKSKEAIASLNPMCTLRYSATHVEKHHMLYKLDS
                     VDAYEQKLVKQIEVAGIEVKDGHNKAFIKLVSVNNKKSPISAKIEIDARLKNGSIKRK
                     EVTVTSGADLLDAKYSGGRDVYDGYIIDDIYCEQGNEYISFTSKPDILKLGQALGEVD
                     PDEFKRLQIRKTIEEHLDKEMRLRPQGIKVLSLFFIDKVANYRWYDEDGNPQKGKFAL
                     MFEEEYDRAIRKPKYSQLFEGADLDTAAQGVHNGYFAIDKKKDSSGKEMLKESKIGKD
                     GKAASSESDGSAYNTIMKDKEWLLSFDCKLKFIFSHSALKEGWDNPNVFQICTLNETS
                     SVIKKRQEIGRGLRICVNQNGERVHGFAVNTLTVMANESYEQFAEQLQKEIENEEGIK
                     FGVVEKHLFANIVVPVDDHNHEYLGSEASKQLWEHLQSEGHIDGKGKVQDSLKASLKN
                     GTLELPEAFQPHTAQIVSVLKKVSGNLNIKNRDDKAKVSLNKAVYLSPEFQALWDRIK
                     YKTTFRVNFDTRALIEKCAEEIKINLQVGKARFTYRKAKTEIDRGGVHTEKTVEATSV
                     YESRSYDLPDLITYLQNETNLTRRTIVSIINKSGRLESFKNNPQKFIEQAGNIIKSQM
                     RLFIVDGIKYHKIGDDQFYAQELFQQNELFGYLKDNMVKAEKSVFDYVVYDSDIELEF
                     ASAFERNEDIKLYAKLPGWFKIDTPLGGYNPDWAVLIEKDGKEKLYFVVETKSTLFSV
                     ERRPEENAKIKCGYAHFEALGDEVKFTVSNSMDEFSSRYA"
     gene            complement(5351..7291)
                     /locus_tag="SI621_003365"
     CDS             complement(5351..7291)
                     /locus_tag="SI621_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEI4066035.1"
                     /translation="MKNVIPEMDSQSMDIVADNISKLKELFPEAFIEGKVDFDALKEV
                     LGDYIDGREERYSFTWNGKSKARMLAQTPSAGTLRPCKEESVDWDNTQNLFIEGDNLE
                     VLKLLQKSYHKKVKMIYIDPPYNTGKDFVYKDNFKDNIKNYKEITGQVDGEGRNLSNN
                     PETSGRYHTDWLNMMYPRLKLARNLLKDDGVIFISIDDNEFANIRKIADEIFGEENFI
                     ASIVWQGGRKNDSRRFSVVHDYILVYVKNENYLSAREVKWRERKEGLDQVYEKHKALL
                     KKHGSNYDEASKELKEWFNDLGENDPSKEHSHYSKMDEGGVYYGDNISSPNYRENLIF
                     EYKGYKPPKNGWRYNQEAMARLDREGLLIFPSSKESRIQYKRYLHHTETWAPSTFLYK
                     DRRAASKSLSALMGGEFFDFPKDISVLARLIDTFTGESDLVLDFFAGSSAFAHACIEL
                     NVRRSKANRFVMVQLPEITDEKSEAFKAGYKTISDISKERIRRAACQIKDENPDYQGD
                     LGFKVFKLDSTNIKPWELDFDLTEQDLEDQISNIKHGRKEEDVLYEVLLKYGLDLTLP
                     IAEHSVAGHKVFDIGMGALVICLSDDITLDVVEGIVKLKDELNPEIIRVVFKDAGFAD
                     DVVKTNAVQILKQAGIEDVRSL"
     gene            complement(7342..8196)
                     /locus_tag="SI621_003366"
     CDS             complement(7342..8196)
                     /locus_tag="SI621_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HEI4066036.1"
                     /translation="MSDMGLGLPQFEAFLEGLNVPNSCVLNKPLFKKMFQAHADLDAK
                     DKKALKDDVDKIRWLYTLKPSTINIAPFSDDLRDYGELAFLQVELSNTKSAERIGQLI
                     NRAIPYPLVIFFVHAENKGTEGTGDSDSNVREKQHDKEQLAICLAGKRINRADQAKWV
                     IEELQLSPWLGLGCKTQSEQIFFNSLNFTSLPHSNFWQFYQAFMCRVQALQCAEVSGR
                     FCLVDGLVNGEAAEQQRQQLQHYRQIEQEIKKLRAQIKQAEFSQQVVLNTQIKQQEQR
                     LRQIANTL"
     gene            complement(8189..11455)
                     /locus_tag="SI621_003367"
     CDS             complement(8189..11455)
                     /locus_tag="SI621_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEI4066037.1"
                     /translation="MLLDNKTSGHVGNELKKYTFSGSRLSVLSSLFTLYGFASLKHEL
                     KQLESARIIFSEWDSLNLQNLVGSDSDVLLLNQLQQKRIASEFSQWLANKAEVKASVR
                     PQPGQNIIHLEATEQSFAISGSATLSPTGLGDVRSDSLHMNMGLSDPESTRQLLTWFD
                     NVWADSQNFRDIKNNLIAKLDAIAVDQPASFIYFLTLFHLFKDFLEDIDEDNIIRSKT
                     GFKDTLVWNKLYKFQKDGVLGAIDKLEKHNGCIIADSVGLGKTFEALAVIKYYELRND
                     RVLVLCPKKLRDNWTMYTVNDKRNLLATDRFNYDVLNHTDLTRPKGYSGEINLETLNW
                     ANYDLVVIDESHNFRNTPSKAEGKSRYERLMNDIIRSGVKTKVLMLSATPVNNRMNDL
                     KNQVAFITEGRDDAFAEQGIKNIENTLRLAQKQFNQWVSEPVEKRTTASLLDIMSFDY
                     FKLLDIVTIARSRKHIEKYYGTEDIGRFPTRLPPKNIYANIDASSEFPPLKDVNKTIR
                     RLSLAGYSPLKFVREAKKAEYARRYDKAVGGGKGVFKQVDREESLIHLMRVNLLKRME
                     SSIHSFTLTATKLAQQVDRLLAKIDAHESEDLTELNIEDIHDFELESTELESYMVGNK
                     TKVLLQDMDLIRFRQELEADREFLTYIADTAQRVSAARDAKLAQLKQTIASKVANPLN
                     PGNKKVIVFTAFADTAQYLYAHLAQWAHSELGIHTALVTGGGTNKTTMAGLGSDLNNI
                     LTAFSPVSKERSKVDPTATTELDLLIATDCISEGQNLQDCDTLINYDIHWNPVRIIQR
                     FGRIDRLGSKNTQIQLINFWPNMELDEYINLEARVSGRMVLLDISATGEENVIDENAK
                     NMNDLEYRRRQLQQLKDAVVDLEDMSGGVSITDLTLNDFRMDLSGYMNDKARLATLEQ
                     APLGLYAAVSIDQNLAADSIKPGVIFCLKNIRSGKEAVQVDDNYPLAPYFLIYVTADA
                     EGSAVVELNFTQSKKILDLLKREASKHKAPEQPAITEVNRITRHGRDMSYFQHLLAVA
                     VDSIAGKSEEKGIESLFAKGGTVLTATSSQGIEDFMVVSYLILTAATQGTEGQGERDV
                     "
     gene            complement(11470..11649)
                     /locus_tag="SI621_003368"
     CDS             complement(11470..11649)
                     /locus_tag="SI621_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066038.1"
                     /translation="MSEQIMTVKDVASYLKLNERTVYRMAMSVKIPAFKVGTSWRFKR
                     EDIEKWIEEQKNDRQ"
     gene            12039..13247
                     /locus_tag="SI621_003369"
     CDS             12039..13247
                     /locus_tag="SI621_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEI4066039.1"
                     /translation="MDESKLKALAAELAKGLKTEADLNQFSRILTKLTVETALNAELT
                     DHLGHEKNAPKKGSNTRNGYSSKTLLCDDGEIELNTPRDRENTFEPQLIKKNQTRITQ
                     MDSQILSLYAKGMTTREIVATFKEMYDADVSPTLISKVTDAVKDQVTEWQNRQLNALY
                     PIVYMDCIVVKVRQNGSVINKAVFLALGINTEGQKELLGMWLAENEGAKFWLSVLTEL
                     KNRGLQDILIACVDGLKGFPDAINSVYPQTHIQLCLIHMVRNSLKYVAWKDYKAVTSR
                     LKTVYQAPTEEAALMALDSFADDLDEKYPQISKSWRAHWENLNTFFNYPPDIRKAIYT
                     TNAIESLNSVIRAAIKKRKVFPTDDSVRKVIYLAIKDASKKWSMPIQNWRLAMSRFII
                     EFGDRLSDHL"
     gene            13296..14102
                     /locus_tag="SI621_003370"
     CDS             13296..14102
                     /locus_tag="SI621_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066040.1"
                     /translation="MSLPSTFVYEGAIVPTKTTSEIAWHPWLARRITGQLTQPIKARL
                     QQLNTYLFRQTGGDKSLFPGLLGHRERSLLIFGDEKALDSLPPDGWKHVTLTLADMGA
                     FRRAPPLPYESSRCHDLPAIIVENSDVYYRLCQLNRAQTRWSLVIYGAGNKVSGQAEC
                     VSQLLEVEQVEQLLYFGDLDVAGLKIAHQLRCKLLNDYGISLHLDEWLYNKLILNGLM
                     TAEGNANNERFDFELQCGWMPEFVLREMKTLLAIHQRLPQEGVASLGLFI"
     gene            14229..>14458
                     /locus_tag="SI621_003371"
     CDS             14229..>14458
                     /locus_tag="SI621_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066041.1"
                     /translation="MALYNIANKELHALEKTTFTLEGLQERYDLQEAIKKNIDIIAAD
                     CLVISEEFSDWEDSRRRIDLLAIDKQANLVVI"
BASE COUNT         3564 a         3416 c         3425 g         4053 t
ORIGIN      
        1 cgattcgata ttgcttgtat actcacgaga taccaatgga aggaagacca ttatggccct
       61 aacggatact aaagttcgct ctgcgaaacc tgaggagaaa gagtattcac ttgttgacgg
      121 cgacggcatg tccttacttg taaaacctgg cggttcaaag tattggcgat ttcgtttccg
      181 ttttggcggt aaacaacatt tgatggcgtt cggcgtttac ccggatgttt cgctggcaga
      241 tgccaggaag aaaagggaag aggccagaaa gctggtagct gctggtatcg atcctcgtga
      301 gcataagcgt gctgtgaaag aagagcaggc gaaagagatt attactttcg agaaggttgc
      361 cagagagtgg ctcgtaacca accaaaaatg gtcagaagat catgctaatc gtgtaaaaaa
      421 gagcctggag gacaatatct tcccggcaat tggttctcgc aatattgccg aattgggcac
      481 ccgcgatctg ttaattccta ttaaagccgt ggagaaatcc gggcgtcttg aagtggcttc
      541 ccgccttcaa cagcgcacta cggccatcat gcgttatgca gtgcaaagcg ggttaattga
      601 ttacaatccg gcgcaagaga tggcgggggc ggttgcttcc agtaatcgac agcatcgtcc
      661 ggcgctggag ttaaagcgta tcccggaatt actccagaaa atcgatgaat acaccggcag
      721 gccactaacc cgttgggcaa ccgaacttac tctgcttatt tttattcgat ccagtgagct
      781 gcgttttgca cgctggtcag aaatcgattt tgaaacgtca atgtggacta tcccgcctga
      841 gcgagagcct attcccggcg tgaaacattc tcagcgagga tcaaagatgc gtacacctca
      901 tctggtgccg ctttcaaagc aggcgctagc gatcctgaag cagataaaac agttctgtgg
      961 cgaacatgag ctgattttta ttggcgatca cgatccgcgt aagcctatga gcgagaacac
     1021 ggtaaacagt gcgctacggg tgatgggtta tgacactaag gttgaagttt gcggccacgg
     1081 cttccgtaca atggcctgta gttcgttaat tgagtcagga ctatggtcga aggatgcggt
     1141 ggaaaggcag atgagccata tggaacgcaa ttcggtgagg gctgcgtata tccataaagc
     1201 ggagcatctg gatgagcgca agctgatgtt gcagtggtgg gctgattttc tggatgccaa
     1261 tcgggagaga gcgattacgc cgtttgacta tgcgaagatc aatcggggta atggcgagtg
     1321 agttgaatgc cgttattcct cggctgatgt ttaaatttaa aagaccaacc agcccgctta
     1381 ggactggttg gtcgctgtat tttttaccgt tgttatcaga attccatctg ccaggtacag
     1441 agtcgccaga caaaaaggac gtctgcaata atgccttgaa tgtacataaa gttgcgagcc
     1501 tcatcgcaaa tatgtaccca tggattcttc aacgtaatga cctctgacaa aaccctaaag
     1561 caagcgatat caaatatcac catttggcgc aaaggcgaac agcgtgcgcc acataagcca
     1621 ttattgctgc tgcacttcct ttcacgctat cggtagggtt ataaacgtgg ggggaatgag
     1681 cacgttgcta tcccgaatca tcttgagcga cagttcgccg taacagagtt aaatcaggtg
     1741 tggtgcggcg acgtgaccta tatctggaca ggtaaacgct gggcgaacct tgctgttgtt
     1801 ctcgacctat tcgcaagaaa accagttggc tgggcaatgt ctttctcacc ggacagcaga
     1861 ctgaccatca aagcgctgga tatggcgtgg gaaactcgct gtaaaccagc cggagtgatg
     1921 ttccgcagcg tccagggtag tcactataca atcagtcagt tccggaggtt actgtggggt
     1981 tgccggatca ggcagagtac gagccgacgt ggaaactgtt gggacaacag cccgatggaa
     2041 cgcttcttca gaagtctgaa aaacgagtgg gtgccagtga caggttatat aaactttagc
     2101 gaagctgctc atgcgatcac agactatatc gtcgcatatt acagctcgct aaggccgcat
     2161 gactatacgg tgggttaccc ccaaacgaat cggaaaaccg atactggaaa aactctaaag
     2221 cagtggccag ttttagttga ccactacaaa gtctcatttt gacgtccttt tcaaatcgtt
     2281 catttcaaaa aaattgtgaa ttaacactgc tcacgcatac ctgcttgaga actcatccat
     2341 cgaattagat accgtaaact tcacctcatc acccaatgct tcaaagtgtg cataaccaca
     2401 tttgattttg gcattttcct ccggacgtct ttccacggag aagagcgtgc ttttggtttc
     2461 gacaacgaag tagagttttt ccttgccatc cttttcgatt agaaccgccc agtcggggtt
     2521 atagccgcct aatggcgtat cgattttgaa ccaaccgggt agtttggcgt aaagtttgat
     2581 atcctcattt cgctcgaagg ccgaggcaaa ttccagctca atatcggagt cgtaaactac
     2641 atagtcaaaa acggacttct cagctttaac catgttgtct ttcaggtagc caaacaattc
     2701 gttttgttgg aataattcct gagcatagaa ctgatcatca ccaattttgt ggtacttgat
     2761 tccatcgacg ataaacagac gcatttggct tttgattatg ttacctgcct gttcgataaa
     2821 cttctgtggg ttgttcttga acgactccag gcgaccactt ttgttgataa tggaaacaat
     2881 ggtgcggcgc gtcaggttgg tttcgttctg taagtaagtg atcaaatcgg gtaaatcgta
     2941 cgagcgggat tcgtacacgc tggttgcttc aacggtcttc tctgtgtgca cgccaccccg
     3001 atcaatttca gtcttcgcct tgcggtaagt gaagcgagcc ttacccacct gaaggtttat
     3061 tttgatctct tcggcgcact tctcgatcaa tgccctggta tcaaagttca cgcggaacgt
     3121 agttttgtat ttaatacgat cccacagcgc ctgaaactcg ggactcagat acaccgcttt
     3181 gttcagcgaa accttggctt tgtcatcacg gttcttgatg ttcaggttac cggatacctt
     3241 tttcagcacc gacacaatct gtgctgtatg gggctggaat gcctcaggca actccagagt
     3301 accgttcttc aatgacgcct taaggctatc ctgcacttta cccttgccgt caatgtggcc
     3361 ttcgctctgc aagtgttccc acaattgctt cgacgcctcg ctacccaagt attcatggtt
     3421 gtggtcatca accggcacta cgatattggc aaacaggtgc ttttcaacca caccaaactt
     3481 gataccttct tcgttttcaa tttccttctg caattgctct gcaaattgct cgtaggattc
     3541 attggccatc acggtgagag tattcaccgc aaagccgtgt acccgctcac cattctgatt
     3601 tacacagata cgcaagcccc ggccgatctc ctggcgtttt ttgatcactg agctggtttc
     3661 gttcagggtg cagatctgga acacattagg gttgtcccag ccttccttga gcgctgaatg
     3721 ggaaaaaatg aatttcagtt tgcaatcaaa actcaacaac cattccttgt ctttcataat
     3781 ggtgttgtaa gcactgccat cagattcgct gcttgccgcc ttgccatctt ttccaatttt
     3841 agattctttg agcatttcct tgcctgacga atcttttttc ttatcgatcg caaaataacc
     3901 gttatgcacg ccttgtgcag ctgtatccag atcggcacct tcaaataact ggctgtactt
     3961 gggtttgcga atggcgcgat cgtattcttc ctcaaacatc agggcgaact tacccttttg
     4021 cgggttaccg tcttcgtcat accagcggta gttggcgacc ttgtcgataa aaaacaggct
     4081 cagcacctta atgccctgtg ggcgcaggcg catttcttta tcgagatgct cttcaatggt
     4141 tttgcggatc tgcaggcgtt taaattcatc cggatcaact tctcccaagg cctgacccag
     4201 tttcagaata tcgggcttac tggtaaagct gatgtattca ttgccctgtt cgcagtagat
     4261 gtcatcaata atgtagccgt catatacatc gcggccacca ctgtacttgg catccagcaa
     4321 atccgctccg ctggtcaccg tgacttcctt gcgcttaatg ctgccattct tcaggcgagc
     4381 atcaatttcg attttggcac tgatcgggct ttttttattg ttcacactga ccagcttgat
     4441 aaaggcctta ttgtggccgt ccttaacctc aatgcctgcc acttcaatct gcttaaccag
     4501 tttttgctcg taggcatcaa ctgaatcaag cttgtagagc atatggtgct tttccacatg
     4561 ggtagctgaa taacgcagtg tacacatggg attaagcgag gcgatggcct ctttactctt
     4621 atctgtggtg tcaacactct gcggctcatc gacaatcaca attgggttgg tttgctggat
     4681 aaattcgatc ggcttggcac cggtcatccg gtcgtgtgcc cggtgaatga tgttggcttt
     4741 gttctcttgc tccgggtcgc taaagctttt ccggaaggca tcgatattga tgaccatgat
     4801 ctgaatgtta gggctggtcg caaagttacg cacatccgac agcttactgg aatcgtaggt
     4861 gaaatagtcg aaggcaacgt tttcgtacag ctctttaaaa tgagtcgccg taatttccag
     4921 tgacttctgc actccttctt taatggcgat ggacggcacc acgataataa acttggtaaa
     4981 gccatagagc cggttcattt cgaaaactgt acgcaggtaa acataggttt tacccgttcc
     5041 ggtttccatt tccacggtta agtgaatgcc gtatttcgag tcaaatgact ctgacggcgc
     5101 gaggccattt ttcagctgta ttttgcggat attggcatgg atatcttcat ccagcaggcg
     5161 taggcggtta ccaataccca gggtatcttc ctgaatacca tgaaacagcg agccttcttc
     5221 ccaggaatgc agcggtgcta ccgtaaagtt ggtctggcag atttcctggc cggcgaatac
     5281 gcccgttact gactcaatcg catcacgctg ataatccaga ttggcatcga actgaatctt
     5341 catgctcata ttacaggctc cttacgtctt caattccggc ctgcttgaga atctgtaccg
     5401 cgttggtttt taccacgtcg tcggcaaacc cggcgtcttt gaaaaccacc cgtataattt
     5461 ctgggttgag ttcgtctttg agcttgacaa tcccttccac cacatcgagg gtaatatcat
     5521 ccgacaggca aatcacgagc gcgcccatac cgatatcaaa caccttatgg ccagccacac
     5581 tgtgctcggc aattggcaag gtcagatcca gaccgtactt cagcagcacc tcgtagagca
     5641 catcctcttc tttacgacca tgtttaatat tggatatctg atcttccaga tcctgctccg
     5701 taagatcgaa atccagctcc caaggtttga tattggtgga atcaagtttg aagactttaa
     5761 agccaaggtc accctgataa tctgggtttt catctttaat ttgacacgcc gctcggcgaa
     5821 tgcgttcttt gctgatatcc gagatggttt tataaccagc tttgaatgcc tctgattttt
     5881 cgtcagtaat ttccgggagt tgaaccatta caaatcgatt cgctttactt ctccttacat
     5941 tcaactcaat gcatgcatgc gcaaaagctg aagatccagc aaagaaatca agaaccaaat
     6001 cactctcccc tgtgaaagtg tcaatcagac gagcaagaac ggaaatgtct tttggaaagt
     6061 cgaaaaattc accacccatt agtgcagaca aactcttcga tgcagctcgt ctgtctttat
     6121 atagaaaagt gctaggcgcc catgtctccg tgtgatgcaa atatcttttg tactgaattc
     6181 ggctctcttt tgaggatgga aatatcaaaa gtccttcccg atctagacga gccattgcct
     6241 cttggttata cctccaaccg ttttttggtg gcttataacc tttgtattca aatatcaggt
     6301 tctctctata attcggacta ctgatattgt cgccataata aacgccgcct tcatccattt
     6361 ttgaataatg tgaatgttcc ttggaaggat cattttcacc caaatcattg aaccactctt
     6421 ttagttcctt agatgcttcg tcgtaattgg agccgtgctt ttttagcaga gctttatgct
     6481 tttcatatac ctgatcgagt ccttctttcc tctctcgcca tttgacttct ctcgctgata
     6541 gataattttc atttttaaca taaaccaaaa tatagtcatg tactacagag aatctacgtg
     6601 agtcattttt ccggccgcct tgccatacaa tgctagcgat aaagttttct tcgccaaaaa
     6661 tctcatcggc aattttcctt atattggcaa actcattgtc gtcaatactg ataaaaataa
     6721 ccccatcatc ctttagtaga ttacgagcaa gtttcaggcg tgggtacatc atgttcagcc
     6781 agtcagtgtg atagcgccca ctcgtctccg gattgttaga taagtttcgc ccctcaccat
     6841 ccacctgacc agtgatctct ttgtagtttt tgatattgtc tttaaagtta tctttataaa
     6901 caaaatcctt gcctgtgttg tagggcggat cgatatagat cattttgact tttttgtgat
     6961 agctcttttg cagtagcttg agtacttcga ggttatcgcc ttcgataaag agattttgag
     7021 tattatccca atcaacggat tcttctttac agggacgtaa ggttccagcg cttggtgttt
     7081 gtgccagcat gcgggctttg cttttgccat tccaggtgaa gctgtagcgt tcttcgcgac
     7141 catcgatata gtcaccgagc acttctttca gggcatcgaa gtcgactttg ccttcgataa
     7201 aggcttctgg aaacagctct ttgagtttac tgatattatc agcgacgatg tccatgctct
     7261 gtgaatccat ttctggtatg acgtttttca ctgtgtttcc tcttaattct tttgttttcg
     7321 gcttctactt tttcaattcg gttaaagcgt attagcgatc tgcctgagcc gctgttcctg
     7381 ctgtttaatc tgggtattca acacaacctg ctggctgaac tcggcctgtt ttatctgggc
     7441 gcgtagtttt ttaatttcct gctcgatttg acggtaatgt tgcaattgtt gccgctgctg
     7501 ctctgcagct tcgccattca ctaaaccatc caccaaacaa aagcgcccac tgacttcagc
     7561 acactgaagc gcttgtacac ggcacataaa tgcttggtag aactgccaga aattactgtg
     7621 cggcaagctg gtaaagttca ggctgttaaa aaaaatttgt tcactttgag ttttgcagcc
     7681 caaccctagc caaggtgata gctgtaactc ttcaatgacc cactttgctt gatccgctct
     7741 gtttatgcgc ttgcccgcca ggcagatagc cagttgttct ttgtcatgtt gtttttcgcg
     7801 tacgtttgaa tccgaatcgc cagtgccctc tgtgcccttg ttttcagcgt gcacaaagaa
     7861 aattactaat gggtaaggta tcgccctgtt gatcagctga ccaattcgtt ctgcgctttt
     7921 ggtattgctt aattccactt gcagaaaggc cagctcacca tagtcgcgca ggtcgtcact
     7981 aaaaggcgcg atattgatgg tgctgggctt tagggtgtag agccagcgga ttttatcaac
     8041 atcgtccttc agagcctttt tgtctttagc gtccaagtcg gcgtgcgcct gaaacatctt
     8101 tttgaacaga ggcttattga gtacacagct attgggcacg ttcaagcctt ccagaaaggc
     8161 ttcgaattgt ggcaagccta accccatatc agacatcacg ctctccttga ccttctgttc
     8221 cctgagtagc ggctgtcaat atcaggtagg acaccaccat aaagtcttca atgccctgac
     8281 tggaggtggc ggtaagtacg gtaccgcctt tggcaaatag gctttcaatg cctttttctt
     8341 cgctcttccc ggcaatgctg tcgacggcaa cggccagcaa atgttggaag tagctcatat
     8401 cacgcccgtg gcgcgttatg cgattaactt cggtgatggc gggttgttcg ggcgctttgt
     8461 gcttagaggc ttcacgcttg agtagatcga gtatcttttt actttgggtg aagttcagct
     8521 caaccacggc gctgccttcg gcatcagcgg tcacgtagat caggaagtac ggggccagcg
     8581 ggtagttgtc atctacctga acagcttctt taccactgcg gatattcttc agacagaaga
     8641 tgacgcccgg tttgatgcta tcggcggcca gattttggtc aatactgacg gcggcataca
     8701 gccctagcgg tgcttgctcc agcgttgcga ggcgggcctt gtcgttcata tagcctgaca
     8761 gatccatgcg gaagtcattg agggtaagat cggtaatgga tacaccaccg ctcatgtcct
     8821 ccagatcgac tactgcgtct ttgagttgct gtagttgtct gcgccggtat tcaaggtcat
     8881 tcatgttttt ggcgttttca tcgatgacgt tctcttcgcc agttgcagat atatccagca
     8941 acaccatacg ccccgagaca cgcgcttcca gattgatgta ttcatccagc tccatgttgg
     9001 gccagaagtt gatgagctgg atttgcgtgt ttttactgcc aagacggtcg atacggccaa
     9061 aacgctgaat gatacgtacc ggattccagt gaatatcgta gttgatgagc gtatcgcaat
     9121 cctgcaggtt ctgaccctca ctgatgcagt cagtggctat cagtaaatcc agttcggtcg
     9181 tggcagttgg gtctactttg ctgcgctctt tggataccgg cgagaaggcg gtaagaatat
     9241 tgttgaggtc agagcctaag cccgccatgg tagttttatt ggtaccgccg cccgttacca
     9301 atgcagtatg aatgcccagt tcggaatgtg cccattgcgc cagatgggca tacaggtatt
     9361 gggcggtatc ggcgaacgcg gtaaacacga tcaccttttt attgccggga tttagcgggt
     9421 tagccacctt gctggcaata gtttgtttaa gctgagccag ctttgcatca cgagcagcac
     9481 tcactcgttg tgcagtatca gcaatgtaag tcagaaactc gcggtcggcc tccagttcct
     9541 gccggaagcg aatcagatcc atatcctgca gcaacacttt ggtcttgttg cccaccatgt
     9601 aactttccag ttcggtggac tccagttcaa agtcgtggat atcctcgata ttcagctccg
     9661 tcagatcttc tgattcgtgg gcatcaattt tggccagcag tcggtcaacc tgctgagcaa
     9721 gcttggtggc cgtcagcgtg aacgagtgaa ttgagctttc cattcgcttg agcaagttga
     9781 cgcgcatgag gtgaatcagg ctttcttcac ggtctacttg tttgaataca cctttaccac
     9841 ctcccacggc tttatcataa cgacgggcgt attcggcttt tttggcttcg cgcacgaatt
     9901 tcaacggcga ataacccgcc agggatagcc gccggatggt tttattgacg tcttttaacg
     9961 gcggaaactc gcttgaggcg tcgatgttgg catagatgtt ttttggtggc aagcgagttg
    10021 ggaagcggcc gatatcttcg gtgccatagt acttttcgat gtgtttgcgg gaacgggcaa
    10081 tggtcacgat atcgagaagt ttgaagtagt caaagctcat gatgtcgagt aaagacgccg
    10141 tggtacgctt ttcgaccggc tcactgaccc actgattaaa ttgcttctgg gcaagccgca
    10201 gggtgttttc aatatttttg ataccctgtt cggcaaaggc atcatcccga ccttcagtaa
    10261 tgaaggcgac ctgatttttc agatcgttca tgcgattgtt gaccggcgtt gccgacaaca
    10321 tcagcacttt ggttttcacg cctgagcgga taatgtcgtt catcaggcgt tcatagcggc
    10381 ttttaccttc cgccttgctc ggtgtgttac ggaagttatg ggattcgtcg atgaccacca
    10441 ggtcgtaatt ggcccagttc agtgtctcca gattgatttc gcccgagtag cctttcggtc
    10501 gggtcagatc ggtatggttc agtacatcgt aattaaagcg gtcagttgcg agcagattgc
    10561 gcttatcgtt gaccgtgtac atggtccagt tgtcacgaag ctttttcggg catagaacaa
    10621 gcacccgatc gttacgcagt tcgtagtatt tgatcacagc caaggcttca aaggttttac
    10681 ccaaacccac gctgtcggca atgatgcagc cgttgtgctt ttcaagcttg tctattgcac
    10741 ccagcactcc gtctttctgg aatttgtaga gtttgttcca gactagggta tctttaaagc
    10801 cagttttact gcggatgatg ttgtcttcgt cgatgtcttc gaggaagtct ttgaacaaat
    10861 gaaatagcgt gaggaaatag ataaaactgg ccggttgatc gactgcgatt gcatccagtt
    10921 tggcaatcag attattctta atatcacgaa aattctgtga gtcagcccag acattatcaa
    10981 accacgtcaa aagctgccgg gtgctctcag ggtcagacaa gcccatgttc atgtgcagag
    11041 agtcggagcg cacgtcaccg agcccagttg gactgagcgt ggcactgccg ctgatggcaa
    11101 aactttgctc ggtagcctca agatgaataa tattctgacc cggctgaggc cgcacactgg
    11161 ctttcacttc agccttgttt gccagccatt gcgagaactc actggcgatg cgtttttgtt
    11221 gcagctgatt cagcaggaga acgtccgaat cagaaccaac taggttctgc agattgagcg
    11281 aatcccattc gctgaaaata atccttgcag attccagctg cttcaactcg tgcttcagcg
    11341 aggcaaaacc gtataaggta aacaggctgg acagaacaga gagcctggaa ccagaaaagg
    11401 tatatttttt cagttcgtta ccaacgtggc cgctggtttt gttatccagt aacacccaaa
    11461 aattccctat tattgtctgt cattcttttg ttcttctatc cacttttcga tgtcttcacg
    11521 cttgaaacgc caggatgtgc ccactttgaa ggcaggtatt ttcactgaca ttgccatacg
    11581 gtaaacagtt cgctcgttga gcttgaggta cgacgcgaca tccttgacgg tcattatttg
    11641 ctcactcatt tgcgttatcc gtactctgtt gagggtaatg tttcagtgtc gaaatagtac
    11701 aaggttgtca tcatacgatc aacattatca cccgttgtca agcagataga agccatccag
    11761 gtcaggggtg aatgactagg aattactacc tagggggagc atacatctac gccaatcgag
    11821 ttagctccag gacagggaca tcgtataatt cttgtgtaat gcaattatgg atactgtcat
    11881 cgtcaaggta cttcgccagc ttgttaatgg cgctcaccgg gtatcgcttc agttcaacag
    11941 ctatctgccg gacagtgcag ccataatgag actgtaattt aaattgcgta tctgcctgtt
    12001 tttgatatgt tcattccaac aacggagaca ggcaaattat ggacgaaagt aaactcaaag
    12061 cgctggctgc tgaactggct aaaggtctta aaaccgaagc tgaccttaac cagttttccc
    12121 gtatactgac gaagcttacc gtcgaaacgg cgctcaatgc tgagctgact gaccatctcg
    12181 ggcatgagaa aaatgcgccc aaaaaaggct caaacacccg caatggctac tcatcaaaaa
    12241 ccttgctttg cgatgatggc gagattgagc tgaacacgcc acgtgaccgc gaaaatacct
    12301 ttgaacctca gctgattaag aagaaccaga cgcgtattac gcagatggac agccagatct
    12361 tatctctgta tgccaaaggg atgaccacac gcgaaattgt cgccaccttc aaagaaatgt
    12421 acgacgccga tgtgtcgccc acgctgatat ctaaagtcac tgatgccgtt aaagatcagg
    12481 ttactgagtg gcaaaacaga caacttaatg cgctgtatcc cattgtttat atggactgta
    12541 tcgtcgtgaa agttcggcag aatggtagcg taataaacaa agccgttttt ctggcactgg
    12601 gcatcaacac cgaaggccag aaagagttac tgggtatgtg gctggccgaa aatgaaggcg
    12661 caaagttctg gctgagtgtg ctgacagagc ttaaaaatcg agggcttcag gacatcctga
    12721 ttgcctgcgt ggatggtctg aagggcttcc cggatgcgat aaacagtgtg tatccgcaga
    12781 cgcacatcca gctatgcctc atccatatgg tgcgcaacag tctgaaatat gtggcatgga
    12841 aagactacaa agctgtcacc agcaggctga aaacggtgta tcaggctcca acagaagagg
    12901 cggcgctgat ggcgctggac agcttcgcgg atgacttgga cgaaaaatac ccgcagatca
    12961 gtaaaagctg gcgtgcgcac tgggaaaacc tcaatacatt cttcaactat ccacccgata
    13021 tccgcaaggc gatctacacg acgaatgcca tcgagtcact gaacagcgtg atccgtgcag
    13081 ccataaagaa gcgcaaggtg ttcccgacag acgactcggt acgaaaagtt atttatctgg
    13141 cgatcaagga tgcgtcgaaa aaatggagca tgccgatcca gaactggcgg ctggcgatga
    13201 gtcgttttat tattgagttc ggtgaccgcc tgagcgatca cctttaacga gtgggcagtt
    13261 acacagaatt aaggacaggc tcctactggc ggggtatgag cctgccaagt acttttgttt
    13321 acgaaggtgc catcgttcct acgaagacaa catcagaaat agcctggcat ccctggttgg
    13381 cccgacgtat tacaggacaa cttacccagc caatcaaggc acgcttacaa cagctgaata
    13441 catatctttt tcgccaaacc gggggcgaca agtcgctatt cccgggcttg cttggacatc
    13501 gtgagcggtc tctgcttata tttggtgacg aaaaagctct ggactccttg ccacctgatg
    13561 gctggaagca tgtcactctg actttggctg atatgggcgc gtttcgacgt gcgccgcctt
    13621 taccgtatga gtcatctcgt tgccatgatc ttccggctat cattgttgaa aactccgatg
    13681 tgtattatcg gctatgccaa cttaatcgag cacagactcg ctggtcactg gtcatttatg
    13741 gcgcaggtaa caaagtttcc ggtcaggcgg agtgcgtgtc acaactcctt gaggtcgaac
    13801 aggtagaaca actactttat tttggcgatc tggatgttgc gggcttaaaa atcgctcatc
    13861 agttgcgttg taagttgctt aatgattacg gtatcagctt gcatttagat gaatggcttt
    13921 ataataaact gatcttaaat ggcctgatga cagcggaagg gaacgccaac aacgaacggt
    13981 ttgatttcga gttacagtgt ggctggatgc cagagtttgt tttacgagaa atgaagactc
    14041 ttctggcgat ccaccagcgt ctaccccaag aaggtgtggc ttctttaggt ttatttatat
    14101 agtcataatg tgctttttag gtgatatcat tcccattgaa tgcatcagct taatcgatta
    14161 cagctcagga aataaaattt ttactgtgtt aaaatctctg taaaatcagt taaaaaagga
    14221 agataaacgt ggcgctgtac aatatcgcaa ataaagaact acatgcttta gaaaaaacga
    14281 cctttactct ggaaggatta caggaacgtt atgatttaca ggaagcaatc aaaaaaaata
    14341 tagatattat tgctgctgat tgtctggtga tttccgaaga gttttctgac tgggaagata
    14401 gccgtcggcg aattgattta ttggcaatcg ataaacaggc aaatttagtg gttatcga
//
LOCUS       DARNVM010000123        14352 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.123, whole genome shotgun sequence.
ACCESSION   DARNVM010000123 DARNVM010000000
VERSION     DARNVM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.123"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            182..1126
                     /locus_tag="SI621_003372"
     CDS             182..1126
                     /locus_tag="SI621_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEI4066042.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            1312..2694
                     /locus_tag="SI621_003373"
     CDS             1312..2694
                     /locus_tag="SI621_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEI4066043.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            2731..3453
                     /gene="folM"
                     /locus_tag="SI621_003374"
     CDS             2731..3453
                     /gene="folM"
                     /locus_tag="SI621_003374"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HEI4066044.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(3450..3785)
                     /locus_tag="SI621_003375"
     CDS             complement(3450..3785)
                     /locus_tag="SI621_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HEI4066045.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            3914..4633
                     /gene="rstA"
                     /locus_tag="SI621_003376"
     CDS             3914..4633
                     /gene="rstA"
                     /locus_tag="SI621_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HEI4066046.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDHAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            4637..5938
                     /gene="rstB"
                     /locus_tag="SI621_003377"
     CDS             4637..5938
                     /gene="rstB"
                     /locus_tag="SI621_003377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HEI4066047.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     VAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKMVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            6015..6944
                     /gene="tus"
                     /locus_tag="SI621_003378"
     CDS             6015..6944
                     /gene="tus"
                     /locus_tag="SI621_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HEI4066048.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQRINQLKTTFERIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(6941..8344)
                     /gene="fumC"
                     /locus_tag="SI621_003379"
     CDS             complement(6941..8344)
                     /gene="fumC"
                     /locus_tag="SI621_003379"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEI4066049.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELAMGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSETEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(8487..10133)
                     /gene="fumB"
                     /locus_tag="SI621_003380"
     CDS             complement(8487..10133)
                     /gene="fumB"
                     /locus_tag="SI621_003380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HEI4066050.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10332..11507
                     /gene="manA"
                     /locus_tag="SI621_003381"
     CDS             10332..11507
                     /gene="manA"
                     /locus_tag="SI621_003381"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEI4066051.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPLDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            11608..13116
                     /locus_tag="SI621_003382"
     CDS             11608..13116
                     /locus_tag="SI621_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEI4066052.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYRGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(13161..>14352)
                     /gene="uidC"
                     /locus_tag="SI621_003383"
     CDS             complement(13161..>14352)
                     /gene="uidC"
                     /locus_tag="SI621_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HEI4066053.1"
                     /translation="LADDEAGLRIRLKNELRRADKPSAGAGRDIYAWVQGGLLDFNSG
                     YYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFALGAVKIKPSENSLLKLGRF
                     GTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTPNIDLWGMWRSRVFLWTDST
                     TGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLGASVQKDVSNQIQSILEKSI
                     PLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQLTWNAPWGSVFGSGGYLRHA
                     MNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQFTLTFAPIVTRGYESSKRD
                     VRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGREKRDGSALGDRLNYWDVKMSIQYD
                     FMLK"
BASE COUNT         3524 a         3667 c         3508 g         3653 t
ORIGIN      
        1 tgatgctgat acggctgttt tttaagcata gacggtcatt tgagcaggat taaaattggc
       61 ttaaggaatg tgatatgaaa aatgacgcag acagttacac cgtttaaatg caataatcag
      121 ccacgtttct cgttaataac aataccagta cctggtttgc gcaaggcgaa ggattatttt
      181 tatgaagctt aagaacaccc tcctggcgtc ggcactgctt tctgctacgg cattctccgt
      241 taacgcagca acagaactga caccggagca agcggcagcg gttaaacctt ttgaccgtgt
      301 agtggttacc ggtcgtttta atgctattgg cgaagcggtg aaagccgttt ctcgtcgcgc
      361 agataaagaa ggtgccgcct ctttttatgt tgtcgacact tctgattttg gtaacagcgg
      421 taactggcgt gtggtcgctg acctctataa agccgatgct gaaaaagcag aagaaacaag
      481 taatcgcgta attaacggtg ttgtcgaact gccgaaagat caggctgttc tgattgaacc
      541 gtttgacacg gtcaccgtcc agggcttcta tcgtagccag ccagaagtca atgatgccat
      601 caccaaagcg gcaaaagcga aaggtgccta ctctttctac atcgttcgtc aaatcgatgc
      661 caaccagggc ggcaaccagc gtattactgc attcatctat aaaaaagatg cgaagaaacg
      721 tatcgtccag agcccggatg tgatcccggc agattccgaa gcaggacgtg cagctctggc
      781 tgccggtggc gaagctgcga agaaagttga gatcccgggt gttgcgacta ccgcatcacc
      841 aagttctgaa gtcggtcgct tctttgaaac ccagtcatca aaaggcgggc gttacaccgt
      901 cacgctcccg gatggcacta aagtcgaaga actgaacaaa gcgaccgcag cgatgatggt
      961 cccgttcgac agcatcaaat tctctggcaa ctacggcaac atgaccgaag tctcctatca
     1021 ggttgcgaaa cgtgccgcga agaaaggtgc taagtattac cacatcaccc gccagtggca
     1081 ggaacgtggt aataacctga ccgtcagcgc agatctgtat aaataactat tcagttactc
     1141 ctataaggcg gcttgatgcc gcctttcgca tttttagtca caaatttccc cgcgaccatt
     1201 gcatgcagac acatctcttc gtaaaatccc gcgcttcagt ggcaaactcc atttttatgc
     1261 gttttgccct aatatttatt cttcttgtca cgttttaatc actggatatc gatggaaaag
     1321 aaactgggac tgagcgcact caccgcgctg gtattaagct caatgctggg cgcgggtgtt
     1381 ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg cagcactgct catcggctgg
     1441 ggtattactg gcgctggcat tttattgctg gcctttgcca tgctgatcct cacgcgcatt
     1501 cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg aagggtttgg cgagctaatc
     1561 ggcttttgtt ccgcatgggg atactggctg tgcgcagtca tcgccaacgt ctcctatctg
     1621 gtgatcgttt tttccgcgtt aagctttttt acggacacgc cggaattgcg cctgtttggt
     1681 gatggtaata cctggcaatc gatcgtcggt gcatcggcgt tattgtggat tgttcacttt
     1741 ttgatcctgc gcggagtgca aaccgctgcc agcattaacc tggtggcgac actggcaaag
     1801 ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga tgttcaaact ggataccttc
     1861 aagctcgact tcaccggact tgcacttggc gtacccgtgt gggaacaggt gaaaaacacc
     1921 atgctgatca ccctgtgggt tttcattggt gttgaaggtg ccgtggttgt ttctgcgcgt
     1981 gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg cggttctctc cgctctgggc
     2041 gtttatttgc tggtaacgct gctttcgctg ggcgtggtgg cacgtcctga actggctgaa
     2101 attcgtaacc cgtcaatggc cggtctgatg gtggaaatga tgggaccgtg gggcgaaatc
     2161 atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt acttaagctg gaccatcatg
     2221 gcggcagaag ttccgttcct ggctgccact cataaagcat tcccacgcat ttttgcgcgc
     2281 cagaacgcac aagccgcgcc atccgcctca ctgtggttga ccaacatctg cgtgcaaatt
     2341 tgtctggtgc tcatctggct gaccggttcc gattacaaca cgttgctgac catcgcctca
     2401 gaaatgattc tggtgcccta tttcctggtc ggcgcgttct tgctgaaaat cgcaacccgt
     2461 ccactgcata aagcggtagg tgtcggtgcc tgcatttatg gcttatggtt attgtacgcc
     2521 tctggtccaa tgcacttgct gttatccgtt gttctgtatg cgccggggct gctggttttc
     2581 ctgtatgcgc gtaaaacgca tacacatgat aacgtactga accgtcagga aatggtgcta
     2641 attggcatgc tgttaattgc ctcagttccg gcgacctgga tgctggtggg ataacgttca
     2701 tcccatcgtt ggatagcaaa ggagataacg atgggtaaag cccagccctt gccaatatta
     2761 attactggcg gaggtcgtcg catcggcctc gcactcgcct ggcatttcat taatcaaaag
     2821 caaccggtga ttgtcagcta tcggacgcac tatccagcca ttgatggact gattaaggca
     2881 ggtgcgcagt gtattcaggc tgatttttcg accaacgacg gtgtgatggc gtttgccgat
     2941 gaagtactaa aaagcaccca tggcctgcgt gctattttgc ataacgccag cgcgtggatg
     3001 gcggaaaaac cgggagcgcc actgaccgac gtactggctt gcatgatgca gatccacgtt
     3061 aataccccat atctactcaa ccatgccctg gaaagattac tgcgtgggca cggacacgcc
     3121 gccagcgata tcattcactt taccgattat gtggtggagc gcggtagcga caaacatatt
     3181 gcctatgctg caagcaaagc ggcactggat aatatgaccc gttcgtttgc ccgcaagctg
     3241 gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga tcctgtttaa tgaacatgat
     3301 gatgccgaat atcgacaaca ggcgctgaat aaatcactga tgaaaaccgc gcctggcgag
     3361 aaagaagtga tcgacctggt cgattactta cttaccagct gctttgtcac cggacgcagt
     3421 ttccctcttg atggcggtcg tcatctgcgt taatgcagct taatccagca tataatcaac
     3481 acccatgcgc ttatccccca gcatgcaaca gaaccaacaa aagcggcggg caagcgcatc
     3541 attccggtga aataccacag cgacgccagg taaacaaaat agggaataat cgaccacatg
     3601 ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt cgctggcaac aatataatgc
     3661 gcgatgagcg caaaggtcgg gaaaagtggg atcagcccgg cgatataata atttttcgtt
     3721 tttgctaaaa caccaatcaa cagcactacc agcgcaccga gcgcggcttt gattaccagc
     3781 cccatctttt taccttaaca cttccataac aagtcatcag tagaatacct gatgaaaact
     3841 tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg tattgtgacg tttttatatc
     3901 taccgtgaat aatatgaaca ctatcgtatt tgtggaagat gatgcggaag tcggttcact
     3961 gattgccgcg tacctggcaa aacatgatat gcaggttacc gtagagccgc gcggcgacca
     4021 tgccgaagaa accattttgc gagaaaatcc ggatttggtg ttactcgaca tcatgctacc
     4081 aggcaaggac ggcatgacca tttgtcgtga tttacgcgca aagtggtccg gaccaattgt
     4141 tcttctaacc tctctcgata gcgatatgaa ccacatcctg gcactggaaa tgggagcctg
     4201 cgactatatt ctcaaaacga cgccccctgc tgttttgtta gcgcgtttac gtttgcattt
     4261 gcgtcagaat gagcaagcca cgctgaccaa aggtcttcag gaaacgtcac tgactcccta
     4321 caaagccctg catttcggca cgttgaccat cgatcctatc aaccgcgtag tcaccctggc
     4381 taacactgaa atctcgctct cgacagctga tttcgaatta ttgtgggaat tagctaccca
     4441 tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat ttacgcggcg tcagttatga
     4501 cggcctggat cgtagcgtgg acgtggctat ttcgcggtta agaaaaaaac tgctcgataa
     4561 cgccgcagaa ccttatcgca ttaaaactgt gcgtaacaaa ggctatcttt ttgcgcctca
     4621 tgcatgggaa taagcgatga aaaaactgtt tatccagttt tacctgttat tgtttgtctg
     4681 cttccttgtg atgtctctgc tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg
     4741 caaacagtcg ctggatgatt tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg
     4801 tgagatcccc ccacacgatt ggggtaagac gctgaaggag atggatttaa atctctcttt
     4861 cgatctgcga gtcgagccac tgagtaaata ccatcttgat gatatttcca tgcaccggct
     4921 gcgtggcggc gaaattgtcg ccctggacga tcagtacacg tttttgcaac gtatcccgcg
     4981 cagccactac gtgctggcag ttggtcctgt tccttatctt tattacctcc atcagatgcg
     5041 attgctggat atcgccctga tcgcttttat tgccatttcc ctcgcctttc cggtgtttat
     5101 ctggatgcgt ccgcactggc aggatatgtt aaaactggaa gcagcggcgc aacgatttgg
     5161 cgatgggcat ctcaatgaac gtatccactt tgatgagggt tcgagctttg aacgccttgg
     5221 cgtcgcattt aaccagatgg cggacaatat caacgccttg atagccagca aaaaacagct
     5281 tattgacgga gtcgctcacg aactgcgaac gccgttagtg cgcttgcgtt atcgactgga
     5341 gatgagtgat aacctgagcg ccgccgaatc ccaggcattg aatcgtgata tcagtcaact
     5401 tgaagcttta attgaagagt tgctaactta tgcccgactc gatcgcccac aaaatgagct
     5461 tcatcttagc gaaccagacc tgccgttatg gctgtcaacg catctggcag atattcaggc
     5521 agtaacgccc gataaaatgg tacggataaa aacgctcatg caaggccatt atgcggcgtt
     5581 ggatatgcgc ttaatggagc gcgtgctgga taatttgctc aataacgccc tgcgctactg
     5641 tcactcaacg gttgaaacca gcctgctact gtcggggaat agagcgacat taattgttga
     5701 ggatgatggc ccagggattg cccctgaaaa ccgcgaacat atctttgaac cttttgttcg
     5761 cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggttgg cgattgtcca
     5821 ctctatagca ctagcaatgg gcggtacggt taattgtgac accagcgaac tgggtggtgc
     5881 ccgcttctcg tttagttggc cgttatggca taacatcccg caatttacct ctgcctgaca
     5941 ctatgcgcac ggtggtcagg ctgtcgatac tgtgctataa aataaagtat gttgtaacta
     6001 aagtggttaa tattatggcg cgttacgatc tcgtagaccg actcaacact acctttcgcc
     6061 agatggaaca agaactggct gcatttgccg ctgagctaga acagcacaaa ttgctggttg
     6121 cccgcgtgtt ctctttgccg gaggtaaaaa aagaggatga gcataatccg cttaatcgta
     6181 ttgaggtaaa gcaacatctc ggcaacgacg cgcagtcgct ggcgttacgt catttccgcc
     6241 atttatttat tcaacaacag tccgaaaatc gcagcagcaa ggccgctgtt cgtctacctg
     6301 gcgtattgtg ttaccaggtc gataaccttt cgcaagcagc gttggtcagt catattcagc
     6361 gcatcaatca actcaagact acgttcgagc gtatcgtcac ggttgagtca gaactcccca
     6421 cagcggcgcg ttttgaatgg gtgcatcgtc atttgccggg gctgatcacc cttaatgctt
     6481 accgaacgct cactgttctg cacgaccccg ccactttacg ttttggctgg gccaataaac
     6541 atatcattaa gaatttgcat cgtgatgaag tcctggcaca gctggaaaaa agcctgaaat
     6601 caccgcgcag tgtcgcaccg tggacgcgcg aggagtggca aagaaaactg gagagagagt
     6661 atcaggatat tgctaccctg ccacaggacg cgaagttaaa aatcaaacgt ccggtgaagg
     6721 tgcagccgat tgcccgcgtc tggtacaagg gagatcaaaa acaagtacaa cacgcttgcc
     6781 ctacaccgct tattgcgctg attaatcggg ataatggcgc gggcgtgccg gacgttgggg
     6841 agttgttaaa ttacgatgcc gacaatgtgc aacaccgtta taaaccacag gcgcagccgc
     6901 ttcgtttgat cattccccgg ctgcacctgt atgttgcaga ttaacgccct gctttcatac
     6961 tgccgaccat ctgttctggc cgtacccagc tgtcaaactc ggtttcgcta agatacccca
     7021 gcgcaagggc cgccgctttt aaggtcagcc cttctttatg cgcttttttg gcgatctcgg
     7081 cggctttgtc ataaccaatg tgggtgttga gcgcagtcac cagcatcagc gattcattga
     7141 gtaattgatt gattcgctca cgattcggtt caatacccac tgcgcagtgt ttgttaaaac
     7201 tttccatgcc atctgccagc aagcgcaccg attgcaggaa attgtggatc accatcggac
     7261 ggaagacgtt cagttcaaag ttaccggaag cgccccccat gttgattgcc acgtcgttcc
     7321 ccatcacctg acagcagagc atggttaatg cctcacactg ggttgggttc actttccccg
     7381 gcatgattga gctgcccggc tcattttccg ggattgagat ttcaccaatt ccgcagcgcg
     7441 ggccagaggc cagccagcgg acatcattgg caattttcat cagtgacgca gccaaccctt
     7501 tcaacgcgcc gtgcgcctga accagggcat cacaggtcgc cagcgcttca aatttgttcg
     7561 gcgcggtaac aaacggtgcg caggtaatga ctgccagttc atctgccacg cgacgcgcat
     7621 actccggatg ggtatttagt ccagtaccca cagctgtacc gcccatagcc agttccgcta
     7681 cgtgaggcag gctgtattca atatgtttga gattatgctc gagcatcgct acccagccgg
     7741 aaatctcctg ccccagtgtt agcggcgtgg cgtcctgcaa gtgggttcga ccgattttta
     7801 cgatatcggc aaatgcacgg gatttttcac tcagcgtctg tgtcagggtt ttaagctgcg
     7861 gaatgagttg cttgcgcagc gccagcagcg ccgcaacgtg catcgccgtt ggaaagacat
     7921 cgttagaact ttggcttttg ttcacgtcgt cgttagggtg aactttacgc tccatcccac
     7981 gcacgccgcc gagtaattca ctggcccggt tagccagcac ttcgttcatg ttcatgttac
     8041 tttgcgtgcc ggagccggtc tgccagatag ccaatgggaa ttcgtcgtca tgctgtcctg
     8101 ccagtacttc atccgccgcc tgccgaatgg cgctcgcttt ctcttcagac aacaagccta
     8161 aatcttcatt aacttttgcc gctgcgcgct tggttagcgc cagcgcatga atcagtgagg
     8221 tgggcatttt ctccgtcgaa atgcggaaat gctccagcga gcgttgagtt tgtgcgcccc
     8281 acagcttatc tgccgggaca tcaatcgccc ccatcgaatc tttttcgctg cgtactgtat
     8341 tcatggcctg ctcctcacct gattaatttt ttctttctgt tttgctttcg ttaagcaact
     8401 tttagctcac ttattattta ctatttgata acaaatgttt ggtctttcgt gccatgtaaa
     8461 aaaaccgccc cgaagggcgg ctctgtttat ttcacacagc gggtgcactg tgtgagttgt
     8521 atctgctgga agaagtcatt tcctttatca tccacaagga taaatgccgg gaaatcttcc
     8581 acttcaattt tccagatggc ttccattccc agttccggat attcaacaca ttccaggctc
     8641 ttaatacttc cctgcgccaa tacagctgcc ggaccaccga tactaccgag gtagaaaccg
     8701 ccgtgttttt tacaggcatc cgtcacctgc tggctgcggt tgcctttcgc cagcatgatc
     8761 atacttccgc cctgcgcttg cagttgatcg acataagaat ccatccgtcc ggcggtcgtt
     8821 gggccaagag aaccggaggc ataaccttcc ggcgttttgg ccggacccgc gtagtaaatc
     8881 ggatgatctt taatatactg cggcagccct tcgccgttat ccatccgctc tttcagtttg
     8941 gcgtgagcaa tatcacgacc aacgataatc gtgccattca gcgataagcg tgtagaaacg
     9001 ggatactgcg acaactgtgc gaggatctct ttcatcggac ggttaaggtc aacgcgcacc
     9061 gcttcgcctt ctcccgcttt gcgcagctct tccgggatat atttgcctgg attatgttcc
     9121 agtttttcga tccaaatccc ctgacggttg atctttgctt tgatattacg gtcagcagaa
     9181 caggagacgc ccataccgac cgggcaggat gcgccgtgac gtggcaggcg aatcacgcgg
     9241 atgtcgtgag cgaagtattt accgccaaac tgcgcaccca gaccaagatt ttgcgcttcg
     9301 atcagcaatt ctttttccag ttccacatcg cggaacgcct gtccgtgctc attcccttcc
     9361 gttggcagtt catcatagta tttagcggaa gccagtttca ccgttttaag gttcgtttct
     9421 gcagaagttc caccaataac gaacgcaata tgatacggag gacaggccgc cgtacccagc
     9481 gtgcgcatct tctcaaccag gtaatttttc agtttccccg gcgtcagtaa cgctttggtt
     9541 tcctgataga gatacgtctt gtttgccgaa ccgccacctt tggcgataca gaggaatttg
     9601 tactcgtcgc catcaacggc ataaagatcg atctgcgctg gtagattggt gccggtattc
     9661 acctctttat acatatccag cggcgcgttt tgcgagtagc gcagattatc ttcgatataa
     9721 gtgttataga caccgcgtgc cagcgccgct tcatcaccgc caccggtcca tacacgctgc
     9781 ccttttttac caacaataat cgcggtgccg gtatcctgac aggttggcag aacgcctttt
     9841 gcggcgatgt cggagttgcg caggaattgc agcgccacat atttatcatt ttcgctggct
     9901 tccgggtcac gcagaatgtc ggccacctgt tgttggtgag ccggacgcag cataaatgac
     9961 gcatcatgaa acgcctgacg cgccaacaaa gttaacgctt cgggtgcgac tttcaaaatc
    10021 tcctgccctt caaattcaga tacgctaacg tgttcgctgg ttagcaggta atactcagta
    10081 tcatcttttt tgagtggaaa aggagcctga taatgaaagg gtttgtttga cattgttctc
    10141 tcacttactg cctggtttgg ttatgctctg ggcgggtgtt ccgttgccct gttaaaagcg
    10201 agtaacaata tcctacacac ttttttaaca aaaactgaga ctagtacgac tttttgcggc
    10261 tccaggttac ttcccgtagg attcttgctt taatagcggg attaatttcc acattaaaac
    10321 agggattgat catgcaaaaa ctcattaact cagtgcaaaa ctatgcctgg ggcagcaaaa
    10381 cggcgttgac tgaactttat ggtatggaaa atccgtccag ccagccgatg gccgagctgt
    10441 ggatgggcgc acatccgaaa agcagttcac gagtgcagaa tgccgccgga gatatcgttt
    10501 cactgcgtga tgtgattgag agtgataaat cgactctgct cggagaggcc gttgccaaac
    10561 gctttggcga actgcctttc ctgttcaaag tattatgcgc agcacagccg ctctccattc
    10621 aggttcatcc aaacaaacgc aattctgaaa tcggttttgc caaagaaaat gccgcaggta
    10681 ttccgttgga tgccgccgag cgtaactata aagatcctaa ccacaagccg gagctggttt
    10741 ttgcgctgac gcctttcctt gcgatgaacg cgtttcgtga gttttccgag attgtctccc
    10801 tgctccagcc ggtcgcaggt gcacatccgg cgattgctca ctttttacaa cagcctgatg
    10861 ccgaacgttt aagcgaactg ttcgccagcc tgttgaatat gcagggtgaa gaaaaatccc
    10921 gcgcgctggc gattttaaaa tcggcccttg acagccagca gggtgaaccg tggcaaacaa
    10981 ttcgtttaat ttctgaattt tacccggatg acagcggtct gttctccccg ctgttgctga
    11041 atgtggtgaa attgaatcct ggcgaagcga tgttcctgtt cgctgaaaca ccgcacgctt
    11101 acctgcaagg cgtggcgctg gaagtgatgg cgaactccga taacgtgctg cgtgcgggtc
    11161 tgacgcctaa atacattgat attccggaac tggttgccaa tgtgaagttc gaagccaaac
    11221 cggctaacca gttgttgacc cagccggtga aacaaggtgc agaactggac ttcccgattc
    11281 ctgtggatga ttttgccttc tcgctgcatg accttagtga taaagaaacc accatcagcc
    11341 agcagagtgc cgccattttg ttctgcgtcg aaggcgatgc aacgctgtgg aaaggttctc
    11401 agcagttaca gcttaaaccg ggtgaatcag cgtttattgc cgccaacgaa tcaccggtga
    11461 ctgtcaaagg ccacggccgt ttagcgcgtg tttacaacaa gctgtaagag cttactgaaa
    11521 aaattaacat ctcttgctaa gcttattaaa ggcttataac accttcaggc ggccagtccg
    11581 cctgatttca ttttatggat aatcattatg aataaatcgc tggtagcggt aggcgtcatt
    11641 gttgcgctag gcgtagtctg gacaggcggc gcatggtata caggcaagaa gattgaaacc
    11701 catctcgaag acatggtcgc gcaggcgaac gcgcaactca aactgaccgc tcctgaatcc
    11761 aacctggaag tgagttatca aaactatcat cgcggcgtat tcagcagtca gctgcaacta
    11821 ttggtgaaac ccattgccgg gaaagaaaat ccgtggatta aaagcggtca gagcgtcatc
    11881 ttcaacgaat cagttgatca tggtcccttc ccccttgccc agctaaaaaa actgaacctg
    11941 atcccgtcga tggcatcaat tcaaaccacg ctggttaata acgaagttag caagccactg
    12001 tttgatatgg caaaaggtga aacgcctttt gagattaact cccgcattgg ttacagaggt
    12061 gattccagtt ccgatatttc gctcaagcca ctgaattacg agcaaaagga tgaaaaagtc
    12121 gcctttagcg gcggcgagtt ccagttaaat gcagacagag acggcaaagc tatctccctt
    12181 tccggggagg cgcaaagtgg tcggatagac gcggttaacg aatacaacca gaaagtacag
    12241 ttgaccttta ataatctgaa aaccgacggt tccagcacgc tggcaagttt tggtgagcgc
    12301 gtaggaaatc aaaaactgtc gctggaaaaa atgaccattt cagtggaagg caaagaactg
    12361 gcactgctgg aaggcatgga gatcagcggt aaatcggatc tggtcaatga cggtaaaacg
    12421 atcaatagcc aactggatta ctcgctaaac agcctgaagg tacagaatca ggatctgggc
    12481 agcggcaagc tgactttaaa agtcggccag attgatggtg aagcctggca tcagtttagc
    12541 cagcaatata acgcgcaaac tcaggcgctg ctggcacagc cagaaattgc caacaatccc
    12601 gaactttatc aggagaaagt gacggaagcc ttctttagcg ccctgccgct gatgttgaaa
    12661 ggcgatccgg tgattactat cgcgccgcta agctggaaaa acagtcaggg tgaaagtgcg
    12721 ctgaatctgt cgctgttcct gaaagatccg gcaacgacta aagaagcgcc gcaaacgctg
    12781 gcgcaggaag tagaccgctc ggttaaatct ctggatgcga aactgaccat tccggtggat
    12841 atggcaactg agtttatgac tcaggtagcg aagctggaag gttatcagga agatcaagca
    12901 aaaaaactgg cgaaacagca agttgaaggt gcgtcagcaa tggggcagat gttccgcctg
    12961 accaccttgc aggacaatac catcaccacc agcctgcaat atgctaacgg tcagatcacg
    13021 ttaaacgggc agaaaatgcc actggaagat tttgttggga tgtttgcaat gccggcatta
    13081 aacgttccgg ctgtacccgc tattccgcag cagtaattca cagccaaaac gccggagatt
    13141 tttctctccg gcgttatttg ttacttcagc ataaagtcat actgaatact cattttcaca
    13201 tcccagtaat tcaggcgatc gcccagcgca ctgccatcgc gtttttcccg tcctttatcg
    13261 gcagcaagaa agaaattcat cccttgtaac ggcccttcgc tgacccgata gttcatacca
    13321 cctaagatac ccgcgccttc aatccgcaca tctcgtttac tggattcata gccgcgagtc
    13381 acaatcggtg caaatgtcag cgtaaattgc ggcgttaaac gatagttgac acccaattgc
    13441 caggactgca ttccttcacg gttacgatca agacttaatg aaaagggata gccaatgtcg
    13501 gtatccacca cggcaccatt cattgcatgg cgcaaataac caccactgcc aaatacgctt
    13561 ccccacggcg cattccaggt caattgtcca ctaaccaacg ccgtttcatt gggctggctg
    13621 gtattacgac ttaaaccttc gagttgcgcg taaaagccga gcaactcccc tttcaacgta
    13681 taattcgggt caagcggtat gcttttctcg agaatacttt gtatctgatt ggatacatct
    13741 ttctgtaccg atgcccccag agaatagcga ctggtatcat catgccaact ggcggctaaa
    13801 aaagaacgtg cgcggtgttt atcgtatttt cccgtctggc tgttatacac cccttcatca
    13861 cgaataccgg ttgttgaatc ggtccataaa aatactcgtg aacgccacat tccccacaga
    13921 tcaatatttg gcgttaaagc ccaataacct aatgctcctt cagaaactgt cggtaatgta
    13981 cgttgcgaac tgccagccat taacggaata cgataaggta agctaccata actataatcc
    14041 gtcccgaagc gacctaattt aagtaggcta ttttcactgg gttttatttt tactgctccc
    14101 agggcaaagc caaaactttt atcaccatca agataccacc gggtactcat atcagcacga
    14161 gcacctaatt tataaacata atacgcccca ccttcaacgc caataatatt ggaataataa
    14221 ccactattga aatcgagcaa tcctccctgt acccatgcgt aaatatctct tcccgcgcca
    14281 gcactgggct tatcagccct gcgtaattca tttttcagtc tgatgcgtag tcccgcttca
    14341 tcgtcagcca gt
//
LOCUS       DARNVM010000124        14133 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.124, whole genome shotgun sequence.
ACCESSION   DARNVM010000124 DARNVM010000000
VERSION     DARNVM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.124"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..563
                     /locus_tag="SI621_003384"
     CDS             <1..563
                     /locus_tag="SI621_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4066054.1"
                     /translation="KGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQ
                     DDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFDSS
                     AKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAW
                     MEQIDQATPVTPGAVTANATYVLDYK"
     gene            614..3073
                     /locus_tag="SI621_003385"
     CDS             614..3073
                     /locus_tag="SI621_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEI4066055.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKEKGGIAPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVLPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDN
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDNDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVNIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSNHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVADNVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQLPPLGADIRQEDSGISVGIVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSIHLPEHMEDTANRLILPCH"
     gene            3088..3819
                     /locus_tag="SI621_003386"
     CDS             3088..3819
                     /locus_tag="SI621_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEI4066056.1"
                     /translation="MTFMKGLPLLLLVASLCSYAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQA
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGRQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3816..4877
                     /locus_tag="SI621_003387"
     CDS             3816..4877
                     /locus_tag="SI621_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4066057.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGSSGGSVEKSEVIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGGPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5029..6075
                     /locus_tag="SI621_003388"
     CDS             5029..6075
                     /locus_tag="SI621_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEI4066058.1"
                     /translation="MPILQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLTILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(6072..6863)
                     /gene="nei"
                     /locus_tag="SI621_003389"
     CDS             complement(6072..6863)
                     /gene="nei"
                     /locus_tag="SI621_003389"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HEI4066059.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVETGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLSAARLDALAHALLDIPRLSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6899..7633)
                     /gene="pxpA"
                     /locus_tag="SI621_003390"
     CDS             complement(6899..7633)
                     /gene="pxpA"
                     /locus_tag="SI621_003390"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEI4066060.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRRAMQLPPETVYAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALEQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7623..8555)
                     /gene="pxpC"
                     /locus_tag="SI621_003391"
     CDS             complement(7623..8555)
                     /gene="pxpC"
                     /locus_tag="SI621_003391"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HEI4066061.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8549..9205)
                     /gene="pxpB"
                     /locus_tag="SI621_003392"
     CDS             complement(8549..9205)
                     /gene="pxpB"
                     /locus_tag="SI621_003392"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEI4066062.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9228..9971)
                     /gene="ybgI"
                     /locus_tag="SI621_003393"
     CDS             complement(9228..9971)
                     /gene="ybgI"
                     /locus_tag="SI621_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HEI4066063.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGISVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10242..11723
                     /gene="dtpD"
                     /locus_tag="SI621_003394"
     CDS             10242..11723
                     /gene="dtpD"
                     /locus_tag="SI621_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HEI4066064.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDTSGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11766..13184)
                     /gene="phrB"
                     /locus_tag="SI621_003395"
     CDS             complement(11766..13184)
                     /gene="phrB"
                     /locus_tag="SI621_003395"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEI4066065.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLRIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGVPECVAAPKVRSSGSIDPALSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPTYLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEEFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13181..13690)
                     /locus_tag="SI621_003396"
     CDS             complement(13181..13690)
                     /locus_tag="SI621_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEI4066066.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMAFYPDAWLSEQRYFELWPRV
                     INLRHAGVL"
     gene            complement(13832..14133)
                     /locus_tag="SI621_003397"
                     /pseudo
     CDS             complement(13832..14133)
                     /locus_tag="SI621_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3396 a         3773 c         3446 g         3518 t
ORIGIN      
        1 ttaaaggaca aaaaacattg gccgcactgg ccgtatctct gctgttcact gcacctgttt
       61 atgcggctga tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt
      121 gtgaaataca tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc
      181 acattaatca gtcgcatcac agcgatgccg ttgctgtcga cctgcgctta gtcaactgcg
      241 atctggaaaa ctccaacaac ggttccggcg gcaagatttc taaagttgca gtcacctttg
      301 acagctcagc gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag
      361 cgaccggtgt cggcgtgcgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg
      421 ctactccaga tatcgacctg gcaccaacct ccagcgaaca aacgttaaat ttctttgcgt
      481 ggatggaaca aattgatcag gctacgcccg taacaccagg tgcagttacg gcgaatgcaa
      541 cctacgtgct ggattataaa taagtatcac tttggcggcg ctatcgccgc ctttcttcaa
      601 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttatat cctgcctagt
      661 catggcgata ccttccgcat tggcggtcga attcaacctg aatgtcctcg ataaatcgat
      721 gcgagatcgc attgatattt cattattaaa ggaaaaaggg ggcattgctc ccggtgaata
      781 ttttgttagc gttgcagtaa ataacaacca aatcagtaac ggacaaaaaa ttgactggaa
      841 aaagaacggt gaccaaacca ttccgtgtat taatgattta ctggtagata aatttggttt
      901 aaaacctgaa gttcgtcagt cgttaccacg gttaaaccag tgcgtcgatt ttagctcccg
      961 gcctgaaatt ctcttcattt ttgatcaggc tagccagcaa ctgaatatca ctattccaca
     1021 agcctggctg gcgtggcact cagataactg gactcccccc tcaacatgga aagaaggtgt
     1081 cgccggcgtc ctgatggact acaacctgtt tgccagcagc taccgcccac aggatggtag
     1141 caacagcact aacctgaacg cctatggaac agccggaatt aacgcgggag catggcgttt
     1201 acgcagtgat taccaattga atcagaccga tagcgatgat aaccatgaac agtcaggcga
     1261 aatatcacgc acctatcttt ttcgtccatt accgcaatta ggttcaaaat taacccttgg
     1321 tgaaacggat ttcagttcca atattttcga cggcttttct tataccggcg cggcactggc
     1381 aagtgacgat cggatgttac cgtgggagct acgcggctac gccccacaaa tcagcggcat
     1441 tgcacaaacc aacgccaccg tcaccgtgag tcactcaggg cgagttattt accagaaaaa
     1501 agtcctacca ggtccgttta tcattgatga ccttaatcag tccgtccagg gtacgttgga
     1561 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcgg cagcatcgac
     1621 gcctttcctt acgcgccagg ggcaggttcg ctataaactt gctgccggtc agccgcgaac
     1681 atctatgtct catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
     1741 gctgtcaaat acctcactgt acggcggcct gctgatttcg ggtgacaact accattctgc
     1801 cgcaatgggt atcgggcaaa atatgctctg gcttggtgca ttgtcgtttg atgtcacctg
     1861 ggccagtagt cagtttgata ctcagcagga cgagcggggc ttaagttacc gctttaatta
     1921 cagcaaacaa gtggatgcca ctaacagcac gatttcgctc gccgcttatc gcttctcaga
     1981 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca acgatgcgca
     2041 ggacgaaaaa cagacgatca gcttatccgt ggggcaaccg attaccccac tgaacctcaa
     2101 tctgtacgcc aacctgctgc atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
     2161 catcacggcg ggttttaatg ttaatattgg taactggaga gatatctcga tctcgacgtc
     2221 attcaataca acccattacg aagataaaga tcgcgacaat cagatttacc tgtcgatttc
     2281 gctccccttc ggtaacgggg ggcgggtcgg ttatgacatg caaaacagta atcacagcac
     2341 cacgcaccgc atgtcgtgga atgatacgtt agatgaacgt aatagctggg gcatgtctac
     2401 cggactgcaa tccgaccgtc cggataatgg agcccaggtg agcggtaact atcagcacct
     2461 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
     2521 cagcagcagc tggagcggtt ctttcacggc aactcaatat ggtgcggcgt tccatcgccg
     2581 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
     2641 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
     2701 ttaccagcct tccaccgtgg cggtgaacat gaatgattta cccgacggcg taacagtggc
     2761 agataacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cgctggcttc
     2821 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagc tcccccccct
     2881 cggtgcggat atccgccagg aggatagcgg cattagcgtg ggaatagttg gtgaggaagg
     2941 acacgcctgg ttaagtggag tcgctgaaaa tcaacaattt accgtgatct ggggtgatag
     3001 ccagcgttgc tcgatccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
     3061 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
     3121 ttggttgcca gtctgtgcag ctacgctgcg ctacaacccg atcgcactcg cattgtgttt
     3181 aacgctaatg ataaagccac cagcctgcga gtggataatc gtagcgataa actcccctac
     3241 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
     3301 ctgccaccga tacaacgtct ggaaccgaaa gcaacaacgc aggtgcggat agtcaagcaa
     3361 gcctcgaccg cgaaattacc tggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
     3421 attcctcccg ccccagaaaa aaatagcgac catgccgttc tgcaagtcgc cattcagagt
     3481 cgtatcaagg tattctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
     3541 ctgcagttac aggtcagcca gcaggccaac caactgacac tgaaaaatcc taccgcgtat
     3601 tatctgacca ttgcctatct ggggagaaat gaaaaaggcg ttctccccgg cttcaaaacc
     3661 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaagttatag cggcaggcag
     3721 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
     3781 ggacaatgcc gtttacaagc agtggaggcg aagaaatgag tgctggcaag ggagtgttgc
     3841 tcgtcatctg tctggtattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
     3901 gttcatccgg tggttcggta gaaaaatcag aagtgattca accttttgct gtgccaggta
     3961 atgccaaacc cggcgataag atctgggaat ctgacgatat taaaattccc gtctattgtg
     4021 acaacaatac caacggcaac tttgaaagcg aacatgtcta cgcatgggta aacccctacc
     4081 cgggggtaca ggatcgttat tatcaactcg gcgtgacata caatggtgtc gattacgatg
     4141 ccagtctggg gaaaagccgc atcgacacca accagtgtat cgacagtaag aatattaaca
     4201 tttatacccc tgagcagatt atcgcgatgg gctggcagaa caaaatatgc tcgggtggcc
     4261 ccgctaatat tcacatgtcg cgaacctttc tcgctcgtat gcggttatac gttaagattc
     4321 gcgaaatgcc gccgcatgat tatcaaagta cgcttagcga ctacatcgtc gtgcaatttg
     4381 atggtgccgg tagcgttaac gaagacccta ccgcccaaaa cctgaaatac catattactg
     4441 gtctggaaaa cattcgtgta ctggattgca gcgtcaattt ttctatttca ccagaaacac
     4501 aggtgattga ttttggtaaa tttaacctgc tggatattcg ccgccacaca atgtcgaaaa
     4561 cattcagtat taagacgaca aaatcacaaa atgaccagtg caccgacgga tttaaggtca
     4621 gctcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
     4681 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
     4741 ctgaatacgc cgatttcacc agcgatatgc tgatctacga aaaaacctat acggctgaac
     4801 tttcgtccat tccaggtacc cccatcgagg ctggcccctt cgatacagtg gtgcttttta
     4861 agattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
     4921 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
     4981 tttatgctgt atcgatacac caaagaaaaa ataatgaggg aacgaaggat gccaattttg
     5041 cagtggggaa tgttatgtgt gctctcactt ctcctttcta ttggtttcct cgcggtacat
     5101 ctcccggcgg cgctattact tggaccgatg atcgccggga tcatctttag tatgcgcgga
     5161 attactttac aactcccccg ctccgctttt ctcgccgcgc aggccattct tggctgtatg
     5221 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgtcaactg gccgatcgtg
     5281 cttacaattt tactggtgac gctgctttcc agcgccattg ttgggtggct actggtgcgc
     5341 tatagctcat taccaggaaa tactggtgcc tggggttctt ctcctggcgg cgcggcggca
     5401 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat
     5461 ctgcgagtgc tgttcgtcgc aggggccgct gttctggtca cccgcatgat gctgggcgat
     5521 aacgctgaag cggttaacca gcagatcgtc tggtttccgc cagtgagcat taatctcctg
     5581 cttacgattt tgctcgcggt cgttgctggt acggcgggat gcttgctacg tcttccttcc
     5641 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
     5701 atcgaactac cagaatggct gctggcgatg gcgtatatgg caattggttg gcggattggt
     5761 cttggtttcg ataaacaaat attactgcgg gcattgcgcc cactgccaca aatcctgctg
     5821 tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac ccggtttatg
     5881 catattgatt ttatgactgc ctacctcgcc accagccccg gcgggcttga taccgtggcg
     5941 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
     6001 ctgttcagta ttttgctgac gggacctgcc attgcgcggt ttatttcaac ctatgcgccg
     6061 aaacgtacgg cctagtgctg acagcctggg caccagtaaa acgggcgaga tgacagcgtg
     6121 gttttctcaa tgatgctgcc acagcgttca cacggttcgc catcacgatg aaaaacctta
     6181 aagcgaaaca acgccccatg atgtttattc tcatccacct gcccccgcgt agcgtaagaa
     6241 agtcgaggaa tatccagtaa cgcgtgtgcg agtgcatcta gtcgcgccgc actgagatct
     6301 ttcgctttat gattgccggt taacccaacc tgccagagga tctccacccg caaataattg
     6361 ccaagcccag ccagaaacgc ctgatcgagt agtaatccag caaactgacg gttacgaaag
     6421 cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
     6481 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggggtcaa catctcaata
     6541 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
     6601 cgcgtggtct gcggctcttc gccggtttca accacgcgcc agacgccgta aagctgatta
     6661 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
     6721 acatgggtaa cgtgttgacc gataagtcgt gattgataag attttaactg cgggaaggca
     6781 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
     6841 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt
     6901 atgctgcgac aacaatcccc ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca
     6961 gtgcgtgctc gccatcgcca tgcaggcaga ccgtttgcgc cgtgaccgtt gcccattcgc
     7021 cggtgatgct tttgactctg ccgtgttgca ccatctccaa cgtttgctcc agcgcctgtt
     7081 cttcgttttc aatcaacgcg cctggctggc ttcgcggcac cagcgaaccg tctgcctgat
     7141 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
     7201 tcagctcgct tcctgccagc ccgacgagaa tcaatgccgg atcgcaagcg tataccgctc
     7261 tggcgatggc gtctgccagt tgtgcctctt tcgccgcctg gttgtataac atgccgtgcg
     7321 gtttgacatg acacattacg ccgccttgcg cacgggtaat cgctgccagc gcgccaattt
     7381 gatacagcgt ctgggcgtaa acggtttctg gcggcagctg catggctctg cgaccaaaat
     7441 tttccctgtc gggaaaactc ggatgagcgc caatcgcaac accatttttt attgcttcac
     7501 gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca atattggcag
     7561 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
     7621 ggtcaatttt cattgtgcag ccgccacgct aattgttcga aataacgttg ttgatcttgc
     7681 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
     7741 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgcgg ataaccaccg
     7801 gtggtctgtg cgtcgttcat caatacaatc ggctggccgt tatgcggcac ttgcaccaca
     7861 cccggcaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
     7921 tgtaagcgat agcccattcg gttactttgc gagctaagct gccagggcga acgccagaat
     7981 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
     8041 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgctttgcca
     8101 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg
     8161 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
     8221 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
     8281 aatcgccagc cagtccagac ggcattatca tccagccgcg cttcgcaacc tgcccccgtc
     8341 agggcaaacc acccatcagt ttcgaactct accgttaact gaccgagcgt gatctccagc
     8401 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgtaacgc aggcatatcc
     8461 agtgcgccgc agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
     8521 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
     8581 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
     8641 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
     8701 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgacgtg
     8761 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
     8821 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccac
     8881 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcgcc accgtaaaca accggaattt
     8941 caataaaacg agactccggc tccagcgcct cactctcctc ccaccagcgt tgcaaacgct
     9001 ctatggcatc cagcgccagc gactcaggat tacgtaaaat caccgtgata ttgttcatgc
     9061 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgtgccagt cgccagatcc
     9121 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
     9181 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
     9241 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaacgcg
     9301 cgaataccac cacgttcagt ggcatggtga cccgcagcat aaaaatgcaa tccctgctcg
     9361 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
     9421 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcaac gcgctggacc
     9481 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
     9541 gaggctagtt ccagtcccgg cacaggcatg gtcagttcgc cccacggcac caacggctca
     9601 atttcgccca tgaccgagat ccccagtaac gccgccagct gtgcgttatt gcccagctca
     9661 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcatttgc cagcaacgtt
     9721 tttaaacggt tacgcttcat gccgcgaatg accggagatt cgcctttcca gaagtagcca
     9781 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
     9841 gcagtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
     9901 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
     9961 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    10021 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    10081 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    10141 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    10201 cgcaactatt acgtgacgtt gcagtcaatt gtggaattat tatgaataaa cacgcatcac
    10261 agccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    10321 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    10381 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    10441 ttttggcgga taaagttctc ggtaatcgca tggcggtgat gctgggggcg ttgttgatgg
    10501 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    10561 tggcgattat cgtttgtggc tatggtctgt ttaaatccaa cgtcagttgc ctgctcggcg
    10621 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg
    10681 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtatagct
    10741 gggcgatggg ctttggcctg gcggcggttg gcatgatcgc gggtctggtc attttcttat
    10801 gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa
    10861 actttctcct gcccaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga
    10921 tcaccgtact gttctggaaa gagtggtcgg tatacgcctt gattgtcgcg actattattg
    10981 gcctgggtgt attggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc
    11041 tggggctgat tgttaccctc accttcttca gcatgttgtt ctgggcattc gcacaacagg
    11101 ggggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    11161 ccgttccgac tgctatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    11221 tcctggcgtg ggtggttaaa gagagcgtcg caggtaatcg taccgtgcgc atctggggaa
    11281 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    11341 gctggtccgc aatgtatggt cactcttctc tgccactaat ggtgttaggc ctggcggtga
    11401 tgggctttgc ggaactgttt atcgacccgg ttgctatgtc gcagattacg cgcattgaaa
    11461 tccctggcgt gactggcgta ttaactggca tttatatgct gctttccggc gcgattgcga
    11521 actatctggc tggcgtgatt gccgatcaga catcgcaggc ttcgtttgat acttccgggg
    11581 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    11641 tggcgtgtgt aggagtggtg ttgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    11701 gcgcgctggc gctggagtct taacaggcca gcaccgatgc ctgataaaac gctcgctgcg
    11761 ttttatcagg ctcctttcct cgcttcttcg tacgctgcca gcgttcgcag ccttgcctct
    11821 ttgtgatcaa ctatcggttg cggataatcc agcgtcacac ctgctttctg cgcccacttc
    11881 cacggctcat gcaccacttt ccctggcaca tcgcgcagtt ccggtagcca ctggcggata
    11941 aactcgcctt cgcggtcaaa ttcctcgccc tgggttgtcg ggttaaaaat acgaaaatac
    12001 ggcgctgcat cggttccggt tgaagcggcc cactgccagc caccgttatt ggctgccaaa
    12061 tcaccatcaa tcagctgcga catgaaatat cgctcacctt cgcgccagtc gatcaataaa
    12121 tctttcacca aaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg
    12181 ttaagctgac gcatggcggc atcgacaatc gggtatccgg ttttaccttc ctgccaggcc
    12241 tttaaataag tgggattgct ttgccactgt acacgatccg tccaggcaat aaatggacga
    12301 tgtttacaca acgaagggta atacgtcatc agatgacggt agaactcgcg ccagatcagc
    12361 tcattaagcc agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacagg
    12421 cgatgcaaac actggcgagg cgataacccg cccgttgcca gactggcgga caaacgactg
    12481 gtgccttcca ctgccggaaa atctcgttgt tgctcatact ctccggcacc gttctggcaa
    12541 aactggcgta attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa
    12601 gactgacgag ggtaattcag cgtaattgat agcgcgggat ctatcgatcc actactacgt
    12661 acttttggcg cagcgacgca ctccggcacc ccttctcgca accgtttcag ccaggcgttt
    12721 ttaaatggcg taaagacttt gtacatctca tgattgccag tcatcaccgc ccccggcggc
    12781 aggatcacgc tgtcgtcaaa tccttcacac accacgttac gcagcgttct ctcaacctgc
    12841 acatcccgcg cccgctcatt cacttcatac tgatagttat aaaaaagatg agtaacgctg
    12901 ttttccgcgc acacctgttt aactatttcg acactggcgg caaagtcatc cacttcgcgg
    12961 aacaataaag gaatgccttt ttccgccagc gctattcgta gcccgttcag ttgtgcattg
    13021 ataaattcag cctgacgcgg cgacatatta tgcgccgccc actggcgtgg tgtagcgata
    13081 tacaacgcca gcacgcgtgc agacgaattg cggcaggcgg cagccagcgc gagattatcg
    13141 tgctgacgta aatcctggcg aaaccagacc agatgggtag tcataaaact cctgcatggc
    13201 gcaagttaat cacacgcggc caaagttcga aataacgctg ctctgaaagc caggcgtcag
    13261 gataaaacgc catatagtgt ttgatacgca tcagcggcgc aagaagcggt tgctcgccac
    13321 ggcgataact gtcgataagc gccgccagct gctggcgttc tgtggaatca atatgtgggc
    13381 gaaaatatcc ctgaacgtgc atcaaaacgt tagtatgatc acgccggttg gcagggtgag
    13441 aaagcaaaac aattacgcgt tggcggtact ggttgtaata gtcatccaga ttttgccact
    13501 ggtgaatatc agccacaaac gggccaagtt tgcggtactc cggctgggaa tgggccagaa
    13561 agacgagttt atagcggctg tgataatcgt tgagtgcccc gcgcgttaag ccatgttctt
    13621 tcagtcgatg taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt
    13681 gctgattcat tactgctcct gaacggttta caggagtaag tgtagacgtt gggaaatgat
    13741 atttgtgaag ttaatcctgt tgtagcaggg caagattacg aaagcccgct ccccgcaagg
    13801 actgacgtca ggtagtttct gtccatggct gccctttcca ttcaaacgtg aaatcaataa
    13861 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
    13921 cactcattgc gtaactgtca tgctctggat tatttaattc tttcagtggg aatttttcct
    13981 caaaggcttt atttagtcgt ccctgatttc cttttacagc gaatacataa tcaccgccct
    14041 gtttttgtat cttctctgca atatctttct ggcaacccat cgcatcagtt gtgatgattt
    14101 ttcctttaat atccaacatg ttaagaagtt cag
//
LOCUS       DARNVM010000125        14038 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.125, whole genome shotgun sequence.
ACCESSION   DARNVM010000125 DARNVM010000000
VERSION     DARNVM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.125"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            218..751
                     /locus_tag="SI621_003398"
     CDS             218..751
                     /locus_tag="SI621_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEI4066067.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVFPGMKLSKEKCDQVNAIERDKALVWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRMNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            968..1153
                     /locus_tag="SI621_003399"
     CDS             968..1153
                     /locus_tag="SI621_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HEI4066068.1"
                     /translation="MRKLKMKLCALMLPLVVSACGSMPVAPQPCVKPPAPPAWIMQPS
                     PDWQTPLNGIISPSETD"
     gene            complement(1513..1707)
                     /locus_tag="SI621_003400"
     CDS             complement(1513..1707)
                     /locus_tag="SI621_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEI4066069.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDDG"
     gene            2096..2641
                     /locus_tag="SI621_003401"
     CDS             2096..2641
                     /locus_tag="SI621_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HEI4066070.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2616..4541
                     /locus_tag="SI621_003402"
     CDS             2616..4541
                     /locus_tag="SI621_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEI4066071.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GACQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            4538..4744
                     /locus_tag="SI621_003403"
     CDS             4538..4744
                     /locus_tag="SI621_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HEI4066072.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4741..6342
                     /locus_tag="SI621_003404"
     CDS             4741..6342
                     /locus_tag="SI621_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEI4066073.1"
                     /translation="MKTPTTPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVETAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6323..7654
                     /locus_tag="SI621_003405"
     CDS             6323..7654
                     /locus_tag="SI621_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEI4066074.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNLASDTNKELLNTPEALPV"
     gene            7664..7996
                     /locus_tag="SI621_003406"
     CDS             7664..7996
                     /locus_tag="SI621_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEI4066075.1"
                     /translation="MATKEEFNHYQPLGNSDPAHTAIAPGGLSAKTPAMTPLMLDGTT
                     RKLVVWDGTTDGAAVGILAVAADQTSTTLTFYKSGSFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8052..9077
                     /locus_tag="SI621_003407"
     CDS             8052..9077
                     /locus_tag="SI621_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEI4066076.1"
                     /translation="MSVYTTAELLASTQHHFKFDPLFLRLFFRETYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIRQNMLDENLAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9119..9514
                     /locus_tag="SI621_003408"
     CDS             9119..9514
                     /locus_tag="SI621_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HEI4066077.1"
                     /translation="MTKDELIARLRSLGEQLNRDISLTGTKEELALRVAELEEELDDT
                     GDTAGQDTPLSPENVLTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            9526..9879
                     /locus_tag="SI621_003409"
     CDS             9526..9879
                     /locus_tag="SI621_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HEI4066078.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9891..10469
                     /locus_tag="SI621_003410"
     CDS             9891..10469
                     /locus_tag="SI621_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066079.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSRKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            10466..10861
                     /locus_tag="SI621_003411"
     CDS             10466..10861
                     /locus_tag="SI621_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066080.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10869..11609
                     /locus_tag="SI621_003412"
     CDS             10869..11609
                     /locus_tag="SI621_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066081.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASASVVKGQSTTLTVAFQPEGVTDKSFRAVSSDKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            11625..12047
                     /locus_tag="SI621_003413"
     CDS             11625..12047
                     /locus_tag="SI621_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEI4066082.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLAWLKEQEKKAESSGN
                     LQVSVEDLIRGGAFLVAMSLWHNHPQKTKLPSMNEAITQIEQEVLTTWPTEAIAQAEN
                     VVLRLSGMSEFVVNNSPEQADDAGPAEPVSAGKCSTVS"
     gene            12029..12463
                     /locus_tag="SI621_003414"
     CDS             12029..12463
                     /locus_tag="SI621_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEI4066083.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGQDGNEVIPASPYVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            12456..>14038
                     /locus_tag="SI621_003415"
     CDS             12456..>14038
                     /locus_tag="SI621_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEI4066084.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGITSLAVATGALAYAWYQGDSTLSNFNKTLVLSGNQA
                     GLTADRMLALSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDGAGALQAANEAA
                     TKGFDDQTRKLKDNMGTLETWADKTAQAFKSMWDAVLDIGRPDSSADMLAKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEKRENARLGLSAATLQADLEKASEMAAKD
                     RAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLS"
BASE COUNT         3265 a         3511 c         4265 g         2997 t
ORIGIN      
        1 tgggaattga taaggccaga tatgaaaatg caacagtgag ggcatttaca tcatcccgaa
       61 gcttggaaat gcagtcgagc aactcctgta gagaaatggt gttattgtcc ataaataatc
      121 ctcttgattg tctttacctt ttccccgcct gattcaacag gccgggacag ataaacatat
      181 ccagggttca gaaaccgata aatcctgata aatatccatg aacgcaaaaa tcagatacgg
      241 cctgtcggct gccgttctgg cactgattgc cgtcggtgcg cctgcgcctg atattctcga
      301 ccagtttctg gatgaaaaag aaggtaacca cacaacggca taccgcgatg gttccggcat
      361 ctggaccatc tgtcggggtg ccacggtggt ggatggaaaa cccgtttttc ccggtatgaa
      421 actgtcgaag gaaaaatgcg accaggttaa cgccattgaa cgggataagg cgctggtatg
      481 ggtggagcgc aatattaaag tgccactgac cgaaccacag aaagcgggta tagcgtcatt
      541 ttgtccctat aacattggcc ccggtaagtg tttcccgtcg acgttttata agcggatgaa
      601 tgctggtgat cgtaaaggtg catgcgaggc gattcgctgg tggattaagg acggtggccg
      661 tgattgccgc attcgttcaa ataactgtta cggtcaggtt attcgtcgtg accaggaaag
      721 cgcattagcc tgttggggaa tagatcagtg agcagagtcg ccgcgattat ttatgctctg
      781 gttatctgca ctatcgtctg cctgtcgtgg gcggtcaatc attaccgtga taacgccatc
      841 gcctacaaag aacaacgtga taaaaaagtc agtgagctga agcaggcgat cgccaccatc
      901 gctgacatgc agcagcgtca gcgtgatgtt gctgcgctcg atgcaaagta ctcgagagaa
      961 ttagccaatg cgaaagctga aaatgaaact ctgcgcgctg atgttgccgc tggtcgtaag
     1021 cgcctgcggg tcaatgccag ttgctccgca gccgtgcgtg aagccaccgg ccccaccagc
     1081 gtggataatg caaccatccc ccgactggca gacaccgctg aacgggatta tttcaccctc
     1141 agagaccgat tgatgacgat gcagaagcaa ctggaagggg cacagctata cattcgtgag
     1201 caatgcctca gataaaaacc ggccaaggat aatccgctga agatttgccg gcggcgaaag
     1261 agagccaagg gttcagccta cgccattact cctgtcgatg caatgcattc aatggatgct
     1321 tgatttattt ctgtgcgcta tatcgtcgct gtctcctgca ttaactatga ccgtaacttg
     1381 acggggaacc ccttctgcgt gagtttgcgg gaataatcaa aaacgatgca caccgggttt
     1441 tactgtgctg acagacgcag ggttaccctc atagtcgctt ttccggtgcg atggtggaag
     1501 aaaccgggat gttcatccat catcactttt gattgatgta tatgctctct tttctgacgt
     1561 tagtctccga cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgctc
     1621 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
     1681 tctgcgggtt ctgttcttcg ttgacatgag gttgccccgt attcagtgtc gctgatttgt
     1741 attgtctgaa gttgttttta cgttaagttg atgcaggtca attaatacga tacctgcgtc
     1801 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1861 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggcgacctcg
     1921 cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgccgt
     1981 aacttaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     2041 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     2101 agtcaataaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     2161 gcaggagcag ggaatgcccg ttctgcgagg cggtgggaag ggtaatgagg tgctttatga
     2221 ctctgccgcc gtcataaaat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     2281 gcgccgggag gttgaagaac tgcggcaggc cagcgagaca gatctgcagc cagggactat
     2341 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     2401 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     2461 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     2521 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     2581 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     2641 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     2701 ccgccgttga atgggcggat gccaattact atctcccgaa agaatccgca taccaggaag
     2761 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     2821 tccgtgaggt gaatgtggtg aagtctgccc gtgttggtta ttccaaaatg ctgctgggtg
     2881 tttatgccta ctttatagag cataagcaac gcaacacact tatctggttg ccgacggatg
     2941 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     3001 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc accatgaagc
     3061 gtttcaccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     3121 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgat gatattgaac
     3181 aggaaggctc tccgacgttc ctgggtgaca agcgtattga aggctcggtc tggccaaagt
     3241 ccatccgtgg ctccacgccc aaagtgagag gcgcctgtca gattgagcgt gcagccagtg
     3301 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtacc
     3361 ttaaatttgg tgataaagag acgccgtttg gcctcaaatg gacgccggat gatccctcca
     3421 gcgtgtttta tctctgcgag cataatgctt gcgtcatccg tcagcaggag ctggacttta
     3481 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     3541 tttcgtcatc cggtgaagag attgagccgc cggacagtgt gacctttcac atctggacgg
     3601 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaag acgaaagggg
     3661 atacgggaaa acgtaaaacc ttcgtgaaca ccacgctcgg tgagacatgg gaagcgaaaa
     3721 tcggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     3781 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccaactggac cgctacgaaa
     3841 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgaccgg cagattatta
     3901 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3961 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     4021 ttgacccgac cattgtgtat gaacgctcga aaaaacatgg gctgttccgg gtgatcccca
     4081 ttaaaggggc atccgtctac ggaaagccgg tggccagcat gccacgtaag cgaaacaaaa
     4141 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     4201 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     4261 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     4321 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaagcactcg
     4381 actgcttcgt ttatgcgctg gcagcgctgc gcatcagtat ttcccgctgg caactggatc
     4441 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     4501 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     4561 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cagtacagaa
     4621 agacggacgg cgagtggagt ttacggccac ttccgtgtct gatctgaaaa aatacattgc
     4681 tgagctggaa gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     4741 atgaaaacgc ccaccactcc cacccttctg gggccggacg gcatgacatc actgcgtgaa
     4801 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc atggaaccca
     4861 ccgagtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4921 gacgatctgg tacgcaataa cggctatgcc gccaatgcca tccagctgca tcaggatcat
     4981 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     5041 gaggaagaag cccgagcctt ttcccgcgag gttgaaacgg catggaaaga gtttgccgag
     5101 gatgactgtt gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     5161 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     5221 tcgtcgcggc tgttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     5281 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggcgcg
     5341 gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca
     5401 tggatacccc gtgaattacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     5461 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     5521 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     5581 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     5641 caggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     5701 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     5761 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     5821 tatatcgctg ccgggctggg tgtctcgtat gagcagcttt cccggaatta cgcccagatg
     5881 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5941 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     6001 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     6061 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     6121 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     6181 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     6241 cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cggactgcga
     6301 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     6361 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     6421 gcttgcaggc cagcttggga tcagccgcct gacggatgca gtatccggcg acagcctgac
     6481 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     6541 ccgcagttat caggtcatga acggcatcgc cgtgctgccg gtgtccggta cgctggtcag
     6601 ccggacgcgg gcgctgcagc cgtattcggg aatgaccggt tacaacggca ttatcgcccg
     6661 tctgcaacag gctgccagcg atccgatggt ggacggcatt ctgctggata tggacacacc
     6721 gggcgggatg gtggcgggag catttgactg tgctgacatc atcgcccgtg tgcgagacat
     6781 aaaaccggta tgggcgctgg ccaacgacat gaactgcagt gcaggtcagc tgcttgccag
     6841 cgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6901 ggctcacagt aattacggtg ctgcgctgga gaaacagggc gtggaaatca cgctgattta
     6961 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     7021 aacactgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     7081 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     7141 ggccattgat gccggactgg ctgatgaact tgtgaacagt accgatgcga tcaccgtcat
     7201 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     7261 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     7321 gacggagggc gagaacgcca gcgctgcgca gccggacgtg aacgcgcaga tcaccgcagc
     7381 ggttgcggca gaaaacagcc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     7441 cgaagaacag gcgcgcgttc tggcagaaac ccccggaatg accgtggaaa cggcccgccg
     7501 cattctggct gcagcaccac agagtgcaca ggcgcgcagt gacactgcgc tggatcgtct
     7561 gatgcagggt gcaccggcac cgctggctgc aggtaacctg gcatctgata ccaataaaga
     7621 attacttaat acacctgaag ctttaccggt ataagaggca gttatggcga caaaagaaga
     7681 gtttaaccat taccagccgc tgggtaacag tgatccggct catacagcaa ttgcgcctgg
     7741 cggattgagt gcgaaaacgc ctgcaatgac cccgctgatg ctggatggca ctacccgtaa
     7801 gctggttgtg tgggatggca ccaccgacgg tgcagccgtt ggcattctgg cggttgctgc
     7861 tgaccagacc agcaccacac tgacgttcta caagtccggc tcgttccgtt atgaggatgt
     7921 gctctggccg gaggctgcca gcgacgagac gaaaaaacgg accgcgtttg ccggaacggc
     7981 aatcagcatc gtttaatctt ccccttcatc aacaaaggcc gcctgtgcgg ctttttttat
     8041 ggaaataatt tatgtctgta tatacaactg cagaattact ggcatcgacc cagcatcact
     8101 ttaagttcga tccgctgttt ctgcgcctgt ttttccgtga aacctatcct ttcaccacgg
     8161 aaaaagtcta tctctcacaa attccgggac tggtaaacat ggcgctgtac gtttcgccga
     8221 ttgtttccgg tgaggttatc cgatcccgtg gcggctccac ctctgaattt acgccgggtt
     8281 atgtcaaacc caagcatgag gtgaatccgc agatgaccct gcgtcgcctg ccggatgaag
     8341 atccgcagaa cctggctgac ccggcttacc gtcgtcgccg tattattcgg cagaatatgc
     8401 tggatgaaaa tctggcgatt gcccaggtcg aagagatgca ggcagtttct gccgtgctta
     8461 agggcaaata caccatgacc ggtgaagcct tcgatccggt tgaggtggat atgggccgca
     8521 gtgcggcgaa taacatcacg cagtccggcg gcacggagtg gagcaagcgt gacaagtcca
     8581 cgtatgaccc gaccgacgat atcgaagcct acgcgctgaa cgccagcggt gtggtgaata
     8641 tcatcgtgtt tgatccgaaa ggctgggcgc tgttccgttc cttcaaggcc gtcagggaga
     8701 agctggatac ccgtcgcggc tctcattccg agctggagac agcggtaaaa gacctgggca
     8761 aagcggtgtc ttataaggga atgtatggcg atgtggccat cgtcgtgtat tccggacagt
     8821 acgtggaaaa cggcgtcaaa aagaacttcc tgccggacaa cacgatggtg ctggggaaca
     8881 ctcaggcacg cggtctgcgc acctatggtt gcattcagga tgcggacgca cagcgcgaag
     8941 gcattaacgc ctctgcccgt tacccgaaaa actgggtgac caccggcgac ccggcgcgtg
     9001 agttcaccat gattcagtca gcaccgctga tgctgctggc tgaccctgat gagttcgtgt
     9061 ctgtacaact ggcgtaatca tggcccttcg gggccattgt ttctctgtgg aggagtccat
     9121 gacgaaagat gaactgattg cccgtctccg ctcgctgggt gaacaactga accgtgatat
     9181 cagcctgacg gggacgaaag aagaactggc gctccgtgtg gcagagctgg aagaagagct
     9241 tgatgacacg ggcgacactg ccggtcagga tacccctctc agcccggaaa atgtgctgac
     9301 cggacatgaa aatgaggttg tatcagcgca gccggatacc gtgactgata cggctgatct
     9361 ggtcacggtt gtggcactgg tgacgctgca tactgatgca cttcacgcca cgcgggatga
     9421 ggctgtggca tttgtgctgc cgggaacggc gttccgtgtc tctgccggtg tggcagctga
     9481 aatgacagag cgcggcctgg ccagaatgca ataacgggag gcgctgtggc tgatttcgat
     9541 aacctgttcg atgctgccat tgcctgcgcc gatgaaacga tacgcgggta catgggaacg
     9601 tcagccacca tgacatccgg tgagcagtcc ggtgctgtga tacgtggtgt ttttgatgac
     9661 cctgaaaata tcagctatgc cggacagggc gtgcgcgttg aaggctccag cccgtccctg
     9721 tttgtccgga ctgatgaggt gcggcagctg cggcgcggcg acacgctgac catcggtgag
     9781 gaaaacttct ggatagaccg gatttcgccg gatgatggcg gaagctgtca tctctggctt
     9841 gggcggggcg taccgcctgc cgttaaccgt cgccgctgaa agggggatgt atggccataa
     9901 aaggtcttga gcaggccgtt gaaaacctaa gccgtatcag cagaacggcg gtgcccggtg
     9961 ccgccgcaat ggccattaac cgcgttgctt catccgcgat atcgcagtcg gtggcacagg
    10021 ttgcccgtga gacaaaggta cgccggaaac tggtaaagga aagggccagg ctgaaaaggg
    10081 ccacggtcaa aaatccgcag gccagaatca gggttaaccg gggggatttg cccgtaatca
    10141 agctgggtaa cgcgcgggtt gtcctttccc gccgcagacg tcgtaaaaag gggcagcgtt
    10201 catcccggaa aggtggcggc agcgtgcttg tggtgggaaa ccgtcgtatt cccggcgcgt
    10261 ttattcagca actgaaaaat ggccggtggc atgtcatgca gcgtgtggcc gggaaaaacc
    10321 gttaccccat tgatgtggtg aaaatcccga tggcggtgcc gctgaccacg gcgtttaaac
    10381 agaatattga acggatacgg cgtgaacgtc ttccgaaaga gctgggctat gcgctgcagc
    10441 atcaactgag aatggtaata aagcgatgaa acatactgaa ctccgtgcag ccgtactgga
    10501 tgcactggag aagcatgaca ccggggcgac gctttttgat ggtcgccccg ctgtttttga
    10561 tgaggcggat tttccggcaa ttgccgttta tctcaccggc gctgaataca cgggcgaaga
    10621 gctggacagt gatacctggc aggcggagct gcatattgaa gttttcctgc ctgctcaggt
    10681 gccggattca gagctggatt cgtggatgga gtcccggatt tatccggtga tgagcgatgt
    10741 cccggcactg tcagatttga tcaccagtat ggtggccagt ggctatgact accggcgcga
    10801 cgatgatgcg ggcctgtgga gttcagccga tctgacttat gtcattacct atgaaatgtg
    10861 aggacgatat gcctgtacca aatccagtaa tgccggtgaa aggtgccggg accacactgt
    10921 gggtttataa ggggaacggt gacccttatg cgaacccgct ttcagacgtt gactggtcgc
    10981 gtctggcaaa agttaaagac ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca
    11041 gctatctcga tgatgaggat gcggactgga ctgcgaccgg gcaggggcag aaatctgccg
    11101 gagataccag cttcacgctg gcgtggatgc ccggagagca ggggcagcag gcgctgctgg
    11161 cgtggtttaa tgaaggtgat acccgtgcct ataaaatccg ctttccgaac ggcacggtcg
    11221 atgtgttccg tggctgggtc agcagtatcg gtaaggcggt gacggcgaag gaagtgatca
    11281 cccgcacggt gaaggtcacc aatgtgggac gcccgtcgat ggctgaagat cgcagtacgg
    11341 tgacggcggc aaccggcatg acggtaacgc cagccagtgc gtccgtagtg aaagggcaga
    11401 gcaccacgct gaccgtggca ttccagccgg aaggcgtaac cgacaagagc ttccgtgcgg
    11461 tgtcttcgga taaaacaaaa gccaccgtgt cggtcagtgg tatgaccatc accgtgaacg
    11521 gcgttgccgc aggcaaggtc aacattccgg ttgtatccgg taatggtgag tttgctgcgg
    11581 ttgcagaaat caccgtcacc gccagctaat ccggagagtc agcgatgttc ctgaaaaccg
    11641 aatcatttga atataacggc gtgaccgtca cgctttctga actgtcagcc ctgcagcgta
    11701 ttgagcatct tgcctggttg aaagagcagg aaaaaaaggc tgaatccagc ggcaacctgc
    11761 aggtgtctgt agaggatctt atcagaggcg gggcgtttct ggtggcgatg tctctgtggc
    11821 ataaccatcc gcagaagaca aagctgccgt ccatgaatga agccattacg cagattgagc
    11881 aggaagtgct taccacctgg cccacggagg caattgctca ggctgaaaac gtggtgttac
    11941 gtctgtccgg aatgtctgag tttgtggtga ataattcccc tgaacaggca gatgacgccg
    12001 ggcctgcaga gcctgtttct gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa
    12061 actggcgcgt gagatggggc gacccgactg gcgcgccatg cttgccggga tgtcatccac
    12121 ggagtatgcc gactggcacc gtttttacag tacccattat tttcatgatg ttctgctgga
    12181 tatgcacttt tccgggctga cgtacaccgt gctcagcctg tttttcagcg atccggagat
    12241 gcatccgctg gatttcagtc tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga
    12301 tgtgctgatg cagaaagcgg cagggcttgc cggaggcgtt cgttttggcc aggacgggaa
    12361 tgaagttatc cccgcttccc cgtatgtggc ggacatgacg gaggatgacg taatgctgat
    12421 gacagtatca gaagggatcg caggaggagt ccggtatggc tgaaccggta ggcgatctgg
    12481 tcgttgattt aagtctggat gcggccagat ttgacgagca gatggccaga gtcaggcgtc
    12541 atttttccgg tacggaaagc gatgcgaaaa aaacagcggc agtcgttgaa cagtcaatga
    12601 accggcaggc gctggctgca cagaaagcgg ggatttccgt cgggcagtat aaagctgcca
    12661 tgcgtatgct gcctgcgcag ttcaccgacg tggccacgca gcttgcaggg gggcagaatc
    12721 cgtggctcat cctgctgcaa cagggtggtc aggtgaagga ctccttcggc gggatgatcc
    12781 ccatgttcag ggggcttgcc ggtgcgatca ccctgccgat ggttggtatc acttcgctgg
    12841 cggtggcgac cggtgcgctg gcgtatgcct ggtatcaggg tgactcaacc ctgtccaatt
    12901 tcaataaaac gctggtcctt tccggcaatc aggcgggact gacggcagat cgtatgctgg
    12961 ccctgtccag agccgggcag gcggcagggc tgacgtttaa ccagaccagc gagtcactga
    13021 cggcgctggt gaatgccggt gtgcgtggtg gtgagcagtt tgaggcaatc agccagagtg
    13081 tggcgcgttt ctcctctgca tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga
    13141 agctgaccac tgacccgacg tctgggttga cggcgatggc gcgccagttc cataacgtga
    13201 cggcggaaca gattgcgtat gttgctcagt tgcagcgttc cggcgatggg gccggggcat
    13261 tgcaggcggc gaacgaggcc gcaacgaaag ggtttgatga ccagacccgt aaactgaaag
    13321 ataacatggg tacgctggag acctgggcag acaagactgc acaggcattc aaatccatgt
    13381 gggatgcggt gctggatatt ggtcgcccgg actcatctgc agatatgctc gccaaagctg
    13441 aaaaggcttt tgatgaagcg gataaaaaat ggcagtggta tcagagccga agccaccggc
    13501 gcggtaaaac gtcagcattt cttgccaatc tccggggggc atgggagaag agagagaatg
    13561 cgcgacttgg gctttcagcc gccacgttgc aggccgatct tgaaaaggcc agtgagatgg
    13621 cagcaaaaga tcgggccgag tctgaggcat cacggctgaa atataccgaa gaggcgcaga
    13681 aggcttacga acgcctgcag acaccgctgg agaaatatac cgcccgccag gaggaactga
    13741 ataaggcact gaaagacgga aaaatcctgc aggcagatta caacacgctg atggcggcgg
    13801 cgaaaaagga ctatgaagcg acgctgaaaa agccgaagca gtccggcgtg aaagtgtctg
    13861 cgggcgatcg tcaggaagac agtgctcatg ctgccctgct gacgcttcag gcagaactcc
    13921 ggacactgga gaagcatgcc ggagcgaatg agaaaatcag ccagcagcgc cgggatttgt
    13981 ggaaggcgga aagtcagttc gcggtactgg aggaggcggc gcaacgtcgc cagctgtc
//
LOCUS       DARNVM010000126        13835 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.126, whole genome shotgun sequence.
ACCESSION   DARNVM010000126 DARNVM010000000
VERSION     DARNVM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.126"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            287..508
                     /locus_tag="SI621_003416"
     CDS             287..508
                     /locus_tag="SI621_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066085.1"
                     /translation="MGLWLKIFKIVVGENENYLKDVMMQLEEKNNEGKSVISQANGNP
                     VLKELFWKAVDEFNFPQEELNRLKQYRSL"
     gene            complement(657..2021)
                     /gene="mnmE"
                     /locus_tag="SI621_003417"
     CDS             complement(657..2021)
                     /gene="mnmE"
                     /locus_tag="SI621_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEI4066086.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2127..3773)
                     /gene="yidC"
                     /locus_tag="SI621_003418"
     CDS             complement(2127..3773)
                     /gene="yidC"
                     /locus_tag="SI621_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEI4066087.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3776..4033)
                     /gene="yidD"
                     /locus_tag="SI621_003419"
     CDS             complement(3776..4033)
                     /gene="yidD"
                     /locus_tag="SI621_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEI4066088.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3997..4356)
                     /gene="rnpA"
                     /locus_tag="SI621_003420"
     CDS             complement(3997..4356)
                     /gene="rnpA"
                     /locus_tag="SI621_003420"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEI4066089.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4373..4513)
                     /gene="rpmH"
                     /locus_tag="SI621_003421"
     CDS             complement(4373..4513)
                     /gene="rpmH"
                     /locus_tag="SI621_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEI4066090.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4743..4823)
                     /locus_tag="SI621_003422"
     CDS             complement(4743..4823)
                     /locus_tag="SI621_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066091.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5120..6523
                     /gene="dnaA"
                     /locus_tag="SI621_003423"
     CDS             5120..6523
                     /gene="dnaA"
                     /locus_tag="SI621_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEI4066092.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6528..7628
                     /gene="dnaN"
                     /locus_tag="SI621_003424"
     CDS             6528..7628
                     /gene="dnaN"
                     /locus_tag="SI621_003424"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEI4066093.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7628..8701
                     /gene="recF"
                     /locus_tag="SI621_003425"
     CDS             7628..8701
                     /gene="recF"
                     /locus_tag="SI621_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEI4066094.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8730..11144
                     /gene="gyrB"
                     /locus_tag="SI621_003426"
     CDS             8730..11144
                     /gene="gyrB"
                     /locus_tag="SI621_003426"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEI4066095.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGNIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            11384..11782
                     /locus_tag="SI621_003427"
     CDS             11384..11782
                     /locus_tag="SI621_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEI4066096.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11897..12709
                     /gene="yidA"
                     /locus_tag="SI621_003428"
     CDS             11897..12709
                     /gene="yidA"
                     /locus_tag="SI621_003428"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEI4066097.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12753..13409)
                     /locus_tag="SI621_003429"
     CDS             complement(12753..13409)
                     /locus_tag="SI621_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066098.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGGEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKKFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            13687..>13835
                     /locus_tag="SI621_003430"
     CDS             13687..>13835
                     /locus_tag="SI621_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEI4066099.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFR"
BASE COUNT         3381 a         3545 c         3714 g         3195 t
ORIGIN      
        1 tgttgccgcc tggatggaga cagctgatgt gatagccagc cattatgaaa atgataaaga
       61 tgtggtgaga gaactactgt cgctgagcag aaataatgcc gtctgctctt tgcatattgc
      121 aagctttaag aaaatgagta aacaggtcat tgacgtatat ctgaatgcgg cgattcgcct
      181 ggcgttgcaa cacgggttca ctttcgatga aattgtggag cagtttaccc gtgactttga
      241 tggcaagcca ttctctcatg tggtaaataa cggggatgat atacatatgg gactctggct
      301 gaaaatattc aaaattgtgg ttggtgaaaa tgaaaattac cttaaagatg tgatgatgca
      361 gctggaagaa aagaataatg aaggtaaatc tgtaataagt caggcaaatg gcaatcctgt
      421 acttaaagaa ctattctgga aagcagtaga cgaatttaac tttccgcaag aggagcttaa
      481 taggttaaag caatatcgtt ctctgtaaag acaggactgc aaggtgttta aagtcgctcc
      541 agccagtagc aaatgcagtt ttggtgcagg atgctcataa aaaaacaagc gctgttccgt
      601 tctgatagtt agtttaccca gagcggaaca gtgcccttta tatcaattta gacgacttac
      661 ttaccaatac agaagctgga gaaaatccgc cccagcaggt cgtcagaggt aaactccccg
      721 gtgatctcgc ttaagttctg ctgcgccagg cgcagctctt ccgccagcaa ttcacctgcc
      781 catgcaccca acagttgtgc tttgccctgt tgcagatgct ccgccgcttg ttccagcgcc
      841 tgtaggtgac gacgacgcgc caggaagccg ccttccatgt tggtgtcaaa gcccatgctc
      901 tgtttgagat ggttacgcag cacgtccacg ccttcgccag tccttgccga gagacgaatc
      961 aacgcatgac cgttcacttc actcattccc agcgtttcgc cggtgatatc ggctttattg
     1021 cgcaccacgg tgatcggcag tttcgcgggc agacgggcga taaattccgg ccagatctct
     1081 gccggatcaa cggcgtctgt cgtggtgcca tcgaccataa acagcacgcg gtcggcctgt
     1141 tcaatttcct gccacgcgcg ctcgatacca atacgttcta cttcgtcact ggcttcacgt
     1201 agtccggcgg tatcgatgat atgcagcggc attccgtcaa tgtggatatg ctcacgcagc
     1261 acgtcacgcg tggttccggc gatatcggtt acgattgccg cttcacggcc cgccagcgcg
     1321 tttaacaggc tcgatttacc ggcgttagga cgtccagcaa tcactacttt catcccttcg
     1381 cgcaacaaac taccctgacg tgcttcagca cgcactgcat caagatcggc aattacgtca
     1441 ttgagctggg cttcaatttt gccgtcggag aggaaatcga tctcttcatc agggaaatca
     1501 attgccgctt cgacgtaaat gcgcaagtgg gtgagggctt ccaccagatg gttgacccgt
     1561 gcggagaatg cgccttgcag cgagtttagc gccgaacggg ccgcctgttc cgagctggcg
     1621 tcgataagat ccgcaatcgc ctcagcctgg gctaagtcga gtttatcgtt aagaaacgcg
     1681 cgttcggaaa actcaccagg gcgggcaatc cgcaggccgg gaatggtcag aatgcgtttt
     1741 aacagcaggt cgaggatcac cggaccgcca tgaccctgca gttcaagcac atcttcgccg
     1801 gtgaacgagt tcgggccagg gaaccatagc gcaatcccct gatcgagcac gctgccgtcg
     1861 gcgtctttaa acggaagata atcggcgtag cgcggcttag gcagtttacc cagcacggtt
     1921 tcggcaactt cacgggcttt gaggccggag atgcgcagga tgccaacgcc gccacgtccc
     1981 ggaggcgtgg cctgggctac gatagtgtca ttatcgctca tgatgttcct gttgctttgt
     2041 gtggcggatg cgcggtgctt atccgcccta cgaaaagaaa aaaggcggtc aactgaccgc
     2101 ccttatttta gcgaaaactc accgaatcag gattttttct tctcgcggct atgcaggcca
     2161 cgtttttcca gaccacggta aatcagctgc tgctgaataa tggttaccag gttgctgacg
     2221 atatagtaca gcaccagacc tgacgggaac cacaggaaga acacggtgaa gatgaccggc
     2281 ataaaggtca tgatcttctg ctgcatcggg tcggtcacgg tggtcggcga catcttctga
     2341 atgaagaaca tcgttacgcc catcaggatc ggcaggatgt agtacgggtc ctgtgcagac
     2401 aggtcgtgga tccacagtgc aaacggtgcc tgacgcaatt caacggaacc catcagcatg
     2461 tagtacaacg ccaggaagat tggcatctgg atcagcagcg ggaagcagcc gcccagcggg
     2521 ttaaccttct cagctttgta cagcgccatc atttcctgac tgatacgctg tttgtcatcg
     2581 cccagacgct cacgcattgc ctgaatcttc ggctgcagca tacgcatctt cgccatggag
     2641 gtgtactgcg ctttggtcag cgggtacatg atgccacgaa cgataaaggt gatgatgata
     2701 atggagaagc cccagttacc cacaaagcta tggatccatt tcagcagttt gaacagcggc
     2761 tgagagatga accacaacca accgtaatca acggtcagat ccaggtgcgg agcaacagct
     2821 gccattttgt cctggatttc cgggccaacc cacagggtgc tgttcatcgc gccagtctga
     2881 ccaggctgaa ccagtaccgg ttgagacttg tagccgatag cggcgatgcc gttacccaga
     2941 ttagcggtat agaagttatt ggtaccgtcg ttatgcggga tccacgccgt cgcgaaatac
     3001 tgttgcagca ttgccaccca accacctttc gaagagatgt tcaggttttc gttatcggca
     3061 atggtatcga acttgtattt ctcatacttc tcgtcaggcg tggagtacgc cgcgccacgg
     3121 aaggtgtgca gtgcgaagtt gctgcttccg gtatcgagat gcggtggcag agtgatggat
     3181 tgcttcaact gaccaaaggt ggatatctcc agcggtttct cgccagcgtt ctgcacgttg
     3241 tagttgacgt tgacagcgta atcaccacgt ttcaggacaa acgttttggt aaacgtgttg
     3301 cctgccgcgt cggtatacgt catcggcacc tgcagttcgt tttgaccttc agccagcaca
     3361 taagcgtctt tttcaacgtt atacagcgga cgcgggccgt tagccgggtt atccgggcca
     3421 tcacgaccgg tcagaccgct ctgtgcctga taaataaact gcggtgaagt ttccagcagc
     3481 tggaacggct gggtagagtt cagctctttc gggtaagcag gcagcagagc ttgctcaaca
     3541 tcaccaccac gggtgttgat ggtcagatca agcacgtcgg tcttaaccga gatcagtttc
     3601 ccctggccac tggccggtac gccctggtcg gcggcgctac ccgctgcggt ggtcgttgtc
     3661 tgcgtggtct gttgggcctg aggttgcggg tttttatcct gctcccaggc ttgccagatc
     3721 atgaaagaca cgaacagcaa agcgatgact aaaagattgc gttgcgaatc catcgttagt
     3781 gttctctggt atcaaatggt ccgggcggga cgggatcgtc accaccaggg tgtaaagggt
     3841 ggcattttaa tacgcgtttc accgtcaacc aactgccttt tatcactcca aacctgcgca
     3901 atgcctcaat tccgtagctt gaacaggttg gagtgaaacg acaatgcggc ccgagtagcg
     3961 gactaatcag gcgttgatag acccgaataa gggctatcag gacccgcgag ccaggcgaca
     4021 gtggcggcgc cataattttt ccaacgcttc cgagagagca cggttatcga ggtcggcaac
     4081 cccttttttc gccaccacca cgaaatccat agccgggagt tcatgttggc gcagacggaa
     4141 gctttcacgc gtcagacgtt taatccgatt gcgttcatgg gcgcgtcgaa cgtttttctt
     4201 ggcgactgta agaccgatac ggggatgccc cagcgaattc aggcggccga gaatggtaat
     4261 ttgcggcgtg ccagcccgtt gtggctgctg gaagacgaat gtgaattgac tgggagttaa
     4321 caagcgtaac tccctgggaa atgcgagctt aaccactcag gggttagctt tattacttag
     4381 aaacggtcag acgagcgcgg cctttagcac gacgacgtgc cagaacctga cgaccatttt
     4441 tagtagccat acgagcacgg aagccgtgag aacggttgcg cttcagtaca gacggttgaa
     4501 aagtgcgttt catggcgatt tctacctaaa cttgaataaa ttcaatggct ttattggata
     4561 tccgccgaaa aatgaaacga tggacaccga agccatgggt gattaaagag gccggattgt
     4621 aataattgta cactccggag tcaattctct ttccttattt accgcgcttt tccgcacctt
     4681 ttcgcaggga aaatgtacgg cctcacacca gtgaaaacca gcatggcgcg ccgggtggag
     4741 gattatacgg gctgatgggt aaagcgcaag gatcgtcctg gatctttatt agatcgatta
     4801 agccaatttt tgtctatggt cattaaattt tccaatatgc ggcgtaaatc gtgcccgccc
     4861 tgcggcagga tcgtttacac ttagcgagtt ctggaaagtc ctgtggataa atcgggaaaa
     4921 tctgtgagaa acagaagatc tcttgcgcag tttaggctat gatccgcggt cccgatcgtt
     4981 ttgcaggatc ttgatcgggc atataaccgc agacagcggt tcgtgcgtca ccctcatgca
     5041 gggtcttttc gacgtacgtc aacaatcatg aatgtttcag ccttagtcat tatcgacttt
     5101 tgttcgagtg gagtccgccg tgtcactttc gctttggcag cagtgtcttg cccgattgca
     5161 ggatgagtta ccagccacag aattcagtat gtggatacgc ccattgcagg cggaactgag
     5221 cgataacacg ctggccctgt acgcgccaaa ccgttttgtc ctcgattggg tacgggacaa
     5281 gtaccttaat aatatcaatg gactgctaac cagtttctgc ggagcggatg ccccacagct
     5341 gcgttttgaa gtcggcacca aaccggtgac gcaaacgcca caagcggcag tgacgagcaa
     5401 cgtcgcggcc cctgcacagg tggcgcaaac gcagccgcaa cgtgctgcgc cttctacgcg
     5461 ctcaggttgg gataacgtcc cggccccggc agaaccgacc tatcgttcta acgtaaacgt
     5521 caaacacacg tttgataact tcgttgaagg taaatctaac caactggcgc gcgcggcggc
     5581 tcgccaggtg gcagataacc ctggcggtgc ctataacccg ttgttccttt atggcggcac
     5641 gggtctgggt aaaactcacc tgctgcatgc ggtgggtaac ggcattatgg cgcgcaagcc
     5701 gaatgccaaa gtggtttata tgcactccga gcgctttgtt caggacatgg ttaaagccct
     5761 gcaaaacaac gcgatcgaag aatttaaacg ctactaccgt tccgtagatg cactgctgat
     5821 cgacgatatt cagttttttg ctaataaaga acgatctcag gaagagtttt tccacacctt
     5881 caacgccctg ctggaaggta atcaacagat cattctcacc tcggatcgct atccgaaaga
     5941 gatcaacggc gttgaggatc gtttgaaatc ccgcttcggc tgggggctga ctgtggcgat
     6001 cgaaccgcca gagctggaaa cccgcgtggc gatcctgatg aaaaaggccg acgaaaacga
     6061 cattcgtttg ccgggcgaag tggcgttctt tatcgccaag cgtctacgat ctaacgtacg
     6121 tgagctggaa ggcgcgctga accgcgttat tgctaacgcc aactttaccg gacgggcgat
     6181 caccatcgac ttcgtgcgtg aggcgctgcg cgacttgctg gcattgcagg aaaaactggt
     6241 caccatcgac aatattcaga agacggtggc ggagtactac aagatcaaag tcgcggatct
     6301 cctttccaag cgtcgatccc gctcggtggc gcgtccgcgc cagatggcga tggcgctggc
     6361 gaaagaactg actaaccaca gtctgccgga gattggcgat gcgtttggtg gccgtgacca
     6421 cacgacggtg cttcatgcct gccgtaagat cgagcagctg cgtgaagaga gccacgatat
     6481 caaagaagat ttttccaatt taatcagaac attgtcatcg taaacctatg aaatttaccg
     6541 tagaacgtga gcatttatta aaaccgctac aacaggtgag cgggccgtta ggtggtcgtc
     6601 ctacgctacc gattctcggt aatctgctgt tacaggttgc tgacggtacg ttgtcgctga
     6661 ccggtactga tctcgagatg gaaatggtgg cacgcgttgc gctggttcag ccacacgaac
     6721 caggggcgac gaccgttccg gcgcgcaaat tctttgatat ctgccgtggt ctgcctgaag
     6781 gcgcggaaat tgccgtgcag ctggaaggtg agcgtatgct ggtgcgctcc gggcgtagcc
     6841 gtttttcgct gtctaccctg ccagcggcgg atttcccgaa cctcgatgac tggcagagcg
     6901 aagtcgaatt taccctgccg caggcgacga tgaagcgtct gattgaagcg acccagtttt
     6961 ctatggcgca tcaggacgtt cgctattact taaacggtat gctgtttgaa accgaaggcg
     7021 aagaactgcg caccgtggcg accgacggcc accgcctggc ggtctgctcc atgccaattg
     7081 gtcaatcttt gccaagccat tcggtgatcg tgccgcgtaa aggcgtgatt gaactgatgc
     7141 gtatgctcga cggcggcgac aatccgctgc gcgtgcagat tggcagcaac aacattcgcg
     7201 cccacgttgg cgactttatc ttcacctcga aactggtgga tggtcgcttc ccggattacc
     7261 gacgcgttct gccgaagaac ccggacaaac atctggaagc aggttgcgat ctcctcaagc
     7321 aggcgtttgc tcgcgcggca attctctcta acgagaaatt ccgcggcgta cgtctgtatg
     7381 tcagcgaaaa ccagctgaaa atcaccgcca acaacccgga acaggaagaa gcggaagaga
     7441 tcctcgacgt tacctatagc ggtgcggaga tggaaatcgg cttcaacgtc agctatgtgc
     7501 tggatgttct gaacgcgctg aaatgtgaaa acgtgcgcat gatgctgacc gattcggttt
     7561 ccagcgtgca gattgaagat gccgcatcac agtcggctgc ctatgttgtc atgccaatga
     7621 gactgtaatg tccctcaccc gcttgttgat ccgcgatttc cgcaatatcg aaaccgcgga
     7681 tctcgcttta tctcccggct ttaactttct ggtaggtgcc aacggtagtg gcaaaaccag
     7741 cgtgctggaa gccatctata cgcttggcca tggtcgggcg tttcgcagtt tgcagattgg
     7801 tcgcgtcatt cgccacgagc aggaggcgtt tgttctacac gggcgattac agggcgaaga
     7861 gcgcgagacg gcgattggct taaccaaaga caaacagggc gacagcaaag tccgcatcga
     7921 cggtacagac gggcataagg tcgcggaact ggcgcacctg atgccaatgc agttgataac
     7981 gccagaaggg tttactttac tcaacggcgg ccccaaatac agaagagcat tcctcgactg
     8041 gggatgcttt cacaacgaac ccggattttt caccgcctgg agtaatctca agcgattgct
     8101 caagcagcgc aatgcggcgc tgcgccaggt gacacgttac gaacagctac gcccgtggga
     8161 taaagaactg atcccgctgg cggagcaaat cagtacctgg cgcgcggagt atagcgccgg
     8221 tatcgcggct gatatggccg atacctgtaa gcaatttctc cctgagtttt ctctgacttt
     8281 ctctttccag cgcggctggg agaaagagac agaatatgct gaggtgctgg aacgtaattt
     8341 tgaacgcgat cgccagctaa cctacaccgc gcatggcccg cacaaagcgg acttacgcat
     8401 tcgcgccgac ggtgcgccgg tggaagatac cttatcgcgt gggcagctta agctgttgat
     8461 gtgcgcctta cgtctggcgc aaggagagtt cctcacccgt gaaagcgggc ggcggtgtct
     8521 ctacctgata gatgattttg cctctgagct tgatgatgag cgtcgcgggc tgcttgccag
     8581 ccgcttaaaa gcgacgcaat cacaggtctt tgtcagcgcg atcagtgctg aacacgttat
     8641 agacatgtcg gacgaaaatt cgaagatgtt taccgtggaa aagggtaaaa taacggatta
     8701 acccaagtat aaatgagcga gaaacgttga tgtcgaattc ttatgactcc tccagtatca
     8761 aagtcctgaa agggctggat gcggtgcgta agcgcccggg tatgtatatc ggcgacacgg
     8821 atgacggcac cggtctgcac cacatggtat tcgaggtggt agataacgct atcgacgaag
     8881 cgctcgcggg tcactgtaaa gaaattatcg tcaccattca cgccgataac tctgtctctg
     8941 tacaggatga tgggcgcggc attccgaccg gtattcaccc ggaagagggc gtatcggcgg
     9001 cggaagtgat catgaccgtt ctgcacgcag gcggtaaatt tgacgataac tcctataaag
     9061 tgtccggcgg tctgcacggc gttggtgttt cggtagtaaa cgccctgtcg caaaaactgg
     9121 agctggttat ccagcgcgag ggtaatattc accgtcagat ctacgaacac ggtgtaccgc
     9181 aggctccgct ggcggttacc ggcgagactg aaaaaaccgg caccatggtg cgtttctggc
     9241 caagcctcga aaccttcacc aatgtgaccg agttcgaata tgaaattctg gcgaaacgtc
     9301 tgcgtgagtt gtcgttcctc aactccggcg tttccattcg tctgcgagac aagcgcgacg
     9361 gtaaagaaga ccacttccac tatgaaggcg gcatcaaggc gttcgttgaa tatctgaaca
     9421 agaacaaaac gccgatccac ccgaatatct tctacttctc caccgagaaa gacggtattg
     9481 gcgtcgaagt ggcgttgcag tggaacgatg gcttccagga aaacatctac tgctttacca
     9541 acaacattcc acagcgtgac ggcggtactc acctggcagg cttccgtgcg gcgatgaccc
     9601 gtaccctgaa cgcctacatg gacaaagaag gctacagcaa aaaagccaaa gtcagcgcca
     9661 ccggtgacga tgcgcgtgaa ggcctgattg cggtcgtttc cgtgaaagtg ccggacccga
     9721 aattctcctc ccagaccaaa gacaaactgg tttcttctga ggtgaaatcg gcggttgaac
     9781 agcagatgaa cgaactgctg gcggaatacc tgctggaaaa cccaaccgac gcgaaaatcg
     9841 tggttggcaa aattatcgat gctgcccgtg cccgtgaagc tgcgcgtcgc gcgcgtgaaa
     9901 tgacccgccg taaaggtgcg ctcgacttag cgggcctgcc gggcaaactg gcagactgcc
     9961 aggaacgcga tccggcgctt tccgaactgt accttgtgga aggggactcc gcgggcggct
    10021 ctgcgaagca ggggcgtaac cgcaagaacc aggcgattct gccgctgaag ggtaaaatcc
    10081 tcaacgtcga aaaagcgcgc ttcgataaga tgctctcttc tcaggaagta gcgacgctta
    10141 tcaccgcgct tggttgtggt atcggtcgtg acgagtacaa cccggacaaa ctgcgttatc
    10201 acagcatcat catcatgacc gatgcggacg tcgacggctc gcacattcgt acgctgctgt
    10261 tgaccttctt ctatcgtcag atgccggaaa tcgttgaacg cggtcacgtc tacatcgctc
    10321 agccgccgct gtacaaagtg aagaaaggca agcaggaaca gtacattaaa gacgacgaag
    10381 cgatggatca gtaccagatc tctatcgcgc tggacggcgc aacgctgcac accaacgcca
    10441 gtgcaccggc attggctggc gaagcgttag agaaactggt atctgagtac aacgcgacgc
    10501 agaaaatgat caatcgtatg gagcgtcgtt atccgaaagc aatgctgaaa gagcttatct
    10561 atcagccgac gttgacggaa gctgacctct ctgatgagca gaccgttacc cgctgggtga
    10621 acgcgctggt cagcgaactg aacgacaaag aacagcacgg cagccagtgg aagtttgatg
    10681 tccacaccaa tgccgaacaa aacctgtttg aaccgattgt tcgcgtgcgt acccacggtg
    10741 tggatactga ctatccgctg gatcacgagt ttatcaccgg tggcgaatat cgtcgtatct
    10801 gcacgctggg tgagaaactg cgtggcttgc tggaagaaga tgcgtttatc gaacgtggcg
    10861 agcgtcgtca gccggtagcc agcttcgagc aggcgctgga ctggctggtg aaagagtctc
    10921 gtcgcggtct ctccatccag cgttataaag gtcttggcga gatgaacccg gaacagctgt
    10981 gggaaactac catggacccg gaaagccgtc gtatgttgcg cgttaccgtt aaagatgcga
    11041 ttgctgccga ccagttgttc accacgctga tgggcgacgc cgttgaaccg cgtcgtgcgt
    11101 ttatcgaaga gaacgctctg aaagcggcga atattgatat ttaatgacgt taaccatgcg
    11161 agcgtgcctg atgcgctacg tttatcaggc ctacgagact cctgcaatat attgaatttg
    11221 catgattttg taggccggat cagacgttaa cgccgcatcc ggcaagaagc tataaggaaa
    11281 gggcggagat catcttcgcc ctttttattt ctgcaatccg caattatcat cccgcagatc
    11341 tatacttttt gagtcaacac gtaacgcttt acaggtgatt atcatggggc tttttgatga
    11401 agttgtcggt gcctttctga aaggcgatgc ggggaaatat caggccattt tgagctgggt
    11461 tgaggagcag ggcggcattc aggtgctgct ggaaaaactg caaagtggcg gtttaggtgc
    11521 cattctctca acctggctga gtaatcaaca gggcaatcaa tcggttagcg gcgagcaact
    11581 ggaatcggcg ctcggcacaa atgcggtgtc cgatctcggg caaaaactgg gcgtggatac
    11641 cagtacggct tccagtttac tggcagaaca attgccgaag attattgatg cactctcacc
    11701 gcaaggtgaa gtgtcaccac aagccaataa cgatctgctt tccgcaggca tggaactgct
    11761 gaaagggaaa ctcttccgct aagcaaaagg ggagcacgca acgcgcgttg catccccctt
    11821 ttccctcggg tgttgtactg atttttgagc ggaatcgcgt tagcatgggt caggaaccaa
    11881 tctacctggg gaactcatgg ctatcaaact cattgctatc gatatggatg gcacccttct
    11941 gctgcccgat cacaccattt cacccgccgt taaaaatgcg attgccgcag ctcgcgcccg
    12001 tggcgtgaat gtcgtgctaa cgacgggtcg cccgtatgca ggtgtgcaca attacctgaa
    12061 agagctgcat atggaacagc cgggcgacta ctgcattact tataacggcg cgctggtaca
    12121 gaaggccgcc gatggtagca ccgtggcgca aaccgctctc agctatgacg actaccgttt
    12181 cctggaaaaa ctctcccgcg aagtcggttc tcatttccac gcgctggacc gcaccacgct
    12241 gtacaccgcc aaccgtgata tcagctacta cacggtgcat gaatccttcg ttgccactat
    12301 tccgctggtg ttctgcgaag cggagaaaat ggaccccaat acccagttcc tgaaagtgat
    12361 gatgattgat gaacccgcca tcctcgacca ggccattgcg cgtattccgc aggaagtgaa
    12421 agagaaatat accgtgctga aaagtgcgcc gtacttcctc gaaatcctcg ataaacgcgt
    12481 taacaaaggt acgggtgtga aatcactggc cgacgtgtta ggtattaaac cggaagaaat
    12541 catggcgatt ggcgatcagg aaaacgacat cgcaatgatt gaatatgcag gtgtcggtgt
    12601 ggcaatggat aatgctattc cttcggtgaa agaagtggcg aactttgtca ccaaatccaa
    12661 ccttgaagat ggcgtggcgt ttgctattga gaagtatgtg ctgaattaat cagtgaacgg
    12721 gcaaacggca tgaatgcgtt tgcccgcagc gattaacaat ttctggtcgt gcataaaatc
    12781 aggaactgga gtggtgacgt tactatatcc agcgtaagag tgactggcag cagcgcatta
    12841 acgaggccgc gcgggacgcc atctgttgct ttgtattcat aaaatcccac cgtaaagggt
    12901 tgatggaaaa ccatcacgtt tgagtaatcc gcttttttat ttttctggtg gattgagcct
    12961 ttcaaaccgt ccagcatcca tgtacaacgc cgggtatcac aaataaaacc atattgtgtt
    13021 gccagtgcct tatcttcttc gttgttccag tcgtaataga gcgtaatagc accagagaat
    13081 ttcttattac tgctgcttaa aataaatttc gcgttatcag aaacggtgat tctttcacca
    13141 tgaattgccg gatcgttcag cattttaacc acctcaccgc cgccggtggt caacagatag
    13201 tccaacgatt caccaacaaa aacatagcct ttcgtaccgt tactgtcttc agccagcgat
    13261 aaacccttaa tcgtgtctga ccgccattgc tcggcatgtg gacggcttgc agtattggaa
    13321 accaatcccc aggtgataca tccggtcaac gcaaatgcca aagaacacaa accggcagcc
    13381 ttaaaaaatc ccttaaaatt caacttcatg attaatcctt taaacgagta gctaagccgt
    13441 tgaaagcata ctcttctttc cctcctgttt agcgcctgac atcccttttc agatttctgc
    13501 ccgacgcatg tcattttttt atgcattgtt ctttttatga tctaaattgt agtacaacaa
    13561 tataagtttg tactacatta cacacacagc aaacgcgaac gtcatcacgc tggtactaca
    13621 aagttgccgc gttatgcatc gatcggggta aagtagagaa gaacatacag agcacaagga
    13681 ctctccatga ctctcaataa aaccgatcgc atcgttatta cgctgggtaa acagatcgtt
    13741 cacggcaaat acgtgccagg ctcgccgctt ccggctgagg cggaactctg tgaggagttt
    13801 gcaacctcgc gcaacatcat ccgtgaggtg ttccg
//
LOCUS       DARNVM010000127        13731 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.127, whole genome shotgun sequence.
ACCESSION   DARNVM010000127 DARNVM010000000
VERSION     DARNVM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.127"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            113..1222
                     /gene="livK"
                     /locus_tag="SI621_003431"
     CDS             113..1222
                     /gene="livK"
                     /locus_tag="SI621_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HEI4066100.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1270..2196
                     /gene="livH"
                     /locus_tag="SI621_003432"
     CDS             1270..2196
                     /gene="livH"
                     /locus_tag="SI621_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HEI4066101.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2193..3470
                     /gene="livM"
                     /locus_tag="SI621_003433"
     CDS             2193..3470
                     /gene="livM"
                     /locus_tag="SI621_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HEI4066102.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSISGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3467..4234
                     /gene="livG"
                     /locus_tag="SI621_003434"
     CDS             3467..4234
                     /gene="livG"
                     /locus_tag="SI621_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HEI4066103.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4236..4949
                     /gene="livF"
                     /locus_tag="SI621_003435"
     CDS             4236..4949
                     /gene="livF"
                     /locus_tag="SI621_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HEI4066104.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGNALLANEAVRSAYLGG"
     gene            5068..5814
                     /locus_tag="SI621_003436"
     CDS             5068..5814
                     /locus_tag="SI621_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEI4066105.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6174..7490
                     /gene="ugpB"
                     /locus_tag="SI621_003437"
     CDS             6174..7490
                     /gene="ugpB"
                     /locus_tag="SI621_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEI4066106.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7666..8553
                     /gene="ugpA"
                     /locus_tag="SI621_003438"
     CDS             7666..8553
                     /gene="ugpA"
                     /locus_tag="SI621_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEI4066107.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSFIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8550..9395
                     /gene="ugpE"
                     /locus_tag="SI621_003439"
     CDS             8550..9395
                     /gene="ugpE"
                     /locus_tag="SI621_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEI4066108.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9397..10467
                     /gene="ugpC"
                     /locus_tag="SI621_003440"
     CDS             9397..10467
                     /gene="ugpC"
                     /locus_tag="SI621_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HEI4066109.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10464..11207
                     /gene="ugpQ"
                     /locus_tag="SI621_003441"
     CDS             10464..11207
                     /gene="ugpQ"
                     /locus_tag="SI621_003441"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEI4066110.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGDLNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWADMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11194..11634)
                     /locus_tag="SI621_003442"
     CDS             complement(11194..11634)
                     /locus_tag="SI621_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HEI4066111.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11754..13496
                     /gene="ggt"
                     /locus_tag="SI621_003443"
     CDS             11754..13496
                     /gene="ggt"
                     /locus_tag="SI621_003443"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEI4066112.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLKK
                     GDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(13534..>13731)
                     /locus_tag="SI621_003444"
     CDS             complement(13534..>13731)
                     /locus_tag="SI621_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066113.1"
                     /translation="FSQGWYFCFESQEFLESGDEAARLAGNAPFIIDKDSGEIHSLGT
                     AKPLEEYLQVYEIKKATFGLP"
BASE COUNT         3044 a         3497 c         3927 g         3263 t
ORIGIN      
        1 gctaaatcct taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt
       61 gttaacacat aaaaacaaag caacacaaca tcacgaatgg ggatttttga ctatgaaacg
      121 gaatgcgaaa actatcatcg cagggatgat tgcactggca atttcacaca ccgctatggc
      181 tgacgatatt aaagtcgccg ttgtcggcgc gatgtctggc ccgattgccc agtggggcga
      241 tatggaattt aacggcgcgc gtcaggcgat taaagacatt aatgccaaag ggggaattaa
      301 aggcgataag ctggttggcg tggaatatga cgacgcctgc gacccgaaac aagccgttgc
      361 ggtcgccaac aaaatcgtta acgacggcat taaatacgtt attggtcatc tgtgttcttc
      421 ctctacccag ccagcgtcgg atatctacga agacgaaggt attctgatga tctcaccggg
      481 agcgaccaac ccggaactga cccaacgcgg ttatcaacac atcatgcgta ctgccgggct
      541 ggactcttcc caggggccaa cggcggcaaa atacattctt gagacggtga agccccagcg
      601 catcgccatc attcacgaca aacaacagta tggcgaaggg ctggcacgtt cggtgcagga
      661 cgggctgaaa gcggctaacg ccaacgttgt cttcttcgac ggtattaccg cgggtgagaa
      721 agatttctcc gcgctgatcg cccgcctgaa aaaagaaaat atcgacttcg tttactacgg
      781 cggttactac ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac
      841 tcagtttatg gggccggaag gtgtgggtaa tgcgtcgttg tcgaatattg ctggcgatgc
      901 tgccgaaggc atgttggtca ccatgccaaa acgctatgac caggacccgg caaacaaagg
      961 catcgttgat gcgctgaaag cagacaagaa agatccatcc gggccatatg tctggatcac
     1021 ctacgcggcg gtgcaatctc tggcaactgc ccttgagcgt accggcagcg atgagccgct
     1081 ggcgctggtg aaagatttaa aagctaacgg tgcaaatacc gtgattgggc cgctgaactg
     1141 ggatgaaaaa ggcgatctta agggatttga ttttggtgtc ttccagtggc acgccgacgg
     1201 ttcatccacg gcagccaagt gatcatccca ccgcccgtaa aatgcgggcg ggtttagaaa
     1261 ggttacctta tgtctgagca gtttttgtat ttcttgcagc agatgtttaa cggcgtcacg
     1321 ctgggcagta cctacgcgct gatagccatc ggctacacca tggtttatgg cattatcggc
     1381 atgatcaact tcgcccacgg cgaggtttat atgattggca gctacgtctc atttatgatc
     1441 atcgccgcac tgatgatgat gggcattgat accggctggc tgctggtggc ggcgggattc
     1501 gtcggcgcaa tcgtcattgc cagcgcctac ggctggagta ttgaacgggt agcctatcgc
     1561 ccggtgcgta actctaagcg cctgattgcg ctcatctcgg caatcggtat gtccatcttc
     1621 ctgcaaaact acgtcagcct gaccgaaggt tcgcgcgacg tggcgctgcc cagcctgttt
     1681 aacggtcagt gggtggtggg ccatagcgaa aacttctctg cctctattac caccatgcag
     1741 gcggtgatct ggattgttac tttcctcgcc atgctggcgc tgacgatttt cattcgctat
     1801 tcccgcatgg ggcgcgcctg tcgtgcctgc gcggaagatc tgaaaatggc gagtctgctt
     1861 ggcattaaca ccgaccgggt gattgcgctg acctttgtga ttggcgcggc aatggcggcg
     1921 gtggcgggtg tgttgctcgg ccagttctac ggcgtcatta acccctatat cggctttatg
     1981 gccgggatga aagcctttac cgctgcggtg ctcggtggga ttggcagcat tccgggagcg
     2041 atgattggcg ggctgatcct ggggattgcg gaggcgctct cttctgccta tctgagtacg
     2101 gaatataaag atgtggtctc attcgcccta ctgattctgg tgttgctggt gatgccgacc
     2161 ggtattctgg gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc
     2221 tgctttctgc cgcgatgttc tttgtcctcg ctggcgtctt tatgggcgtg caactggagc
     2281 tggatggcac caaactggtg gtcgacacgg cttcagacat ccgttggcag tgggtgttta
     2341 tcggcacggc ggtggtcttt ttcttccagc ttttgcgacc ggctttccag aaagggttga
     2401 aaagcatttc cggaccgaag tttattctgc ccgccattga tggctccacg gtgaagcaaa
     2461 agctgtttct cgtggcgctg ctggtgcttg cggtggcgtg gccgtttatg gtttcacgtg
     2521 ggacggtgga tatcgccacc ctgaccatga tctacattat tctcggtctc gggctgaacg
     2581 tggtggtagg tctttctggt ctgctggtgc tgggatacgg cggcttttac gccatcggcg
     2641 cttacacttt tgcgctgctc aatcactatt acggtttggg cttctggacc tgtctgccga
     2701 ttgccgggtt aatggcagcg gcggcgggct tcctgctcgg cttcccggtg ttgcgtttac
     2761 gtggcgacta tctggcgatc gttaccctcg gtttcggcga aattgtgcgc atattgctgc
     2821 tcaataacac cgaaattacc ggcggaccga acggtatcag tcagatcccg aaaccgacgc
     2881 tcttcggact cgagttcagc cgtaccgccc gtgaaggcgg ctgggacacg ttcagtaatt
     2941 tctttggcct gaaatacgat ccctccgatc gcgttatctt cctgtacctg gtggcgttgc
     3001 tgctagtggt gttaagcctg tttgtcatta accgcctgct gcggatgccg ctggggcgtg
     3061 cgtgggaagc gttgcgtgaa gatgaaatcg cttgtcgttc gctgggctta agcccgcgtc
     3121 gtatcaagct gactgccttt accatcagcg ctgcgtttgc cggttttgcc ggaacgctgt
     3181 ttgctgcgcg ccagggcttt gtcagccctg aatccttcac ttttgccgaa tcggcgtttg
     3241 tgctggcgat agtggtgctc ggcggtatgg gctcgcaatt tgcggtaatt ctggcggcaa
     3301 ttttgctggt ggtgtcgcgc gaattgatgc gtgatttcaa cgaatacagc atgttaatgc
     3361 tcggtggttt gatggtgctg atgatgatct ggcgtccgca gggcttgctg cccatgacgc
     3421 gcccgcaact caagctgaaa aacggcgcag caaaaggaga gcaggcatga gtcagccatt
     3481 attatctgtt aacggcctga tgatgcgctt cggcggcctg ctggcggtga acaacgtcaa
     3541 tcttgaactg tacccgcagg agatcgtctc gttaatcggc cctaacggtg ccggaaaaac
     3601 cacggtcttc aactgcctga ccggattcta caaacctacc ggcggcacca ttttactgcg
     3661 cgatcagcat ctggaaggtt taccggggca gcaaatcgcc cgcatgggcg tggtgcgcac
     3721 cttccagcat gtgcgtctgt tccgtgaaat gacggtgatt gaaaacctgc tggtggcaca
     3781 gcatcagcaa ctgaaaaccg ggctgttctc cggcctgttg aaaacaccct ctttccgtcg
     3841 cgcccagagt gaagcactcg accgcgccgc cacctggctt gagcgtattg gtttgctgga
     3901 acacgccaac cgccaggcga gcaacctggc ctatggcgac cagcgtcgtc tggagatcgc
     3961 ccgctgcatg gtgacgcagc cggagatttt aatgctcgac gaaccggcag caggtcttaa
     4021 cccaaaagag acgaaagagc tggatgagct gattgccgaa ctgcgtaacc atcacaacac
     4081 cactatcttg ttgattgaac acgatatgaa gctggtgatg gggatttcag acagaattta
     4141 cgtggtcaac caggggacgc cgctggcaaa cggtacgccg gagcagatcc gtaataaccc
     4201 ggacgtgatc cgtgcctatt taggtgaggc ataagatgga aaaagtcatg ttgtcctttg
     4261 acaaagtcag cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca
     4321 atcagggcga gattgtcacc ctcattggcg cgaacggcgc ggggaaaacc accttgctcg
     4381 gcacgttatg cggcgaccca cgcgccacca gcgggcgaat tgtgtttgat gataaagaca
     4441 ttaccgactg gcagacggcg aaaatcatgc gcgaagcggt ggcgattgtc ccggaagggc
     4501 gtcgcgtttt ctcgcggatg acggtggaag agaacctggc gatgggcggc ttttttgccg
     4561 aacgcgacca gttccaggag cgcataaagt gggtgtatga actgtttcca cgtctgcatg
     4621 agcgccgtgt tcagcgggca ggcaccatgt ccggcggtga gcagcagatg ctggcgattg
     4681 gtcgcgcgct gatgagcaac ccgcgtttgt tgttacttga tgagccatcg ctcggtcttg
     4741 cgccgattat catccagcaa attttcgaca ccatcgaaca actgcgcgag caggggatga
     4801 ctatcttcct cgtcgagcag aacgccaacc aggcgctgaa gctcgctgat cgcggctacg
     4861 tgctggaaaa cggccatgtt gtgctctccg atactggtaa tgcgctgctg gcgaacgaag
     4921 cagtgagaag tgcgtattta ggcgggtaaa ttatcgataa cccggcagct aagttctgcc
     4981 gggttattcg tccgtataca gacttgtttc aactgaccag gaggattaaa accagaccac
     5041 ttatttgcca cggaaaggtt ccctcccatg cattatgccg ttgttgtcat gcttgaactg
     5101 ctttgctgta ttcacgctta ccgtaccgga caagagcggt actggatttt tatcattttc
     5161 tgttttccgg tgattggctg cgtggcgtat ttcgtcatag tgatgctgcc tgaaacgggc
     5221 gctgaccgtc acgggcatac tttgctgatg cgcctgcagg acaaactcaa ccctgagcgc
     5281 cacctgcgca aattaaccga agaactggcg attgccgaga ccaatcaaaa ccattatgcg
     5341 ttggctaatg aactggcgcg tctggggcgt tatcacgaag ctgttcccca ttatcagcag
     5401 gcgttaagcg ggatattcgc ccatgaagcg gcaatgatgc taagcctggc gcaggcacag
     5461 tttgctattc aggagttcgc cgcctgtcag cagacgctgg aagatgtgat gcgttataac
     5521 cctgattttc agtcggcaga cgggcatttg ttatttgcca gaacgttagc cgcacaagaa
     5581 aaatacgctg atgcggaaag cgagtttgaa gtattgattt cttattaccc cggcccgcag
     5641 gcgcgtattt actatgcgga aatgttggcg aaaatgagtc ggttacgtga agccaacgag
     5701 cagtatgttg ccgtggttga tacggctaaa cgctcaagac ctcattatag aaaacaccac
     5761 cgggaatgga ttaagaccgc caatgagcgg ctgaagcaga gtgtggtgca atgaaaatct
     5821 tctttgccgt tagaccagtg tcgcggcaat cttttgttaa aattgaaaaa tgtagctctc
     5881 atcgggcagc aaaactggac gcagaatttg ctgccatttt tgctcttcat ggtaaggagt
     5941 tctggcgatt agctggccgt taatcgcctc ttttcagtgc attctcggga tatccgtgcc
     6001 ccatctccca ttttccctca cccgtaccgt caccgccttg tcatctttct gacaccttac
     6061 tatcttacaa atgtaacaaa aaagttattt ttctgtaatt cgagcatgtc atgttacccc
     6121 gcgagcataa aacgcgtgaa ttcgcgcatt cggtacaaca agagagataa acgatgaaac
     6181 cgttacatta tacagcttca gcactggcgc tcggactggc gttaatgggg aatgcacagg
     6241 cagtgacgac cattccgttc tggcattcta tggaagggga actgggtaaa gaggtggatt
     6301 ctctggccca acgttttaac gccgaaaacc cggattacaa aattgtaccg acctataaag
     6361 gcaactacga acagaattta agcgcgggga ttgccgcatt tcgtaccggc aacgcgcctg
     6421 ctattttgca ggtttatgaa gttggcaccg ccaccatgat ggcgtcgaaa gccattaaac
     6481 cggtatatga cgtgtttaaa gaggcgggga ttcagttcga tgagtcgcag tttgtgccga
     6541 cggtttcagg ctactactcc gacagcaaaa ccggccactt actctcccag ccgttcaaca
     6601 gctcgactcc cgttctctat tacaacaaag acgccttcaa gaaagccggt ttagacccgg
     6661 aacagccgcc gaaaacctgg caggatctgg cggactatgc cgcaaaactg aaagcctccg
     6721 gtatgaagtg cggctacgcc agcggctggc agggctggat ccaactggaa aactttagcg
     6781 cctggaacgg tctgccgttt gccagcaaaa acaacggctt tgacggcaca gacgcggtgc
     6841 tggagttcaa caagccggag caggtgaaac acatcgctat gctcgaagag atgaacaaga
     6901 agggcgattt cagctacgtc gggcgtaagg atgaatccac tgagaagttc tataacggtg
     6961 attgcgcgat gaccaccgcc tcttccggtt ctctcgccaa cattcgcgag tacgccaaat
     7021 ttaactacgg cgtaggcatg atgccttacg acgccgatgc gaaagatgcg ccacaaaacg
     7081 ccattatcgg cggagccagc ttgtgggtaa tgcagggtaa agataaagaa acttatacgg
     7141 gtgtggcgaa gttcctcgac ttcctcgcga agccagaaaa cgctgccgag tggcatcaga
     7201 aaactggtta tctgccaatc accaaagcgg cgtatgacct gacccgtgag cagggttttt
     7261 atgagaaaaa cccaggggcg gataccgcga cgcgtcagat gctgaataag ccgccgttac
     7321 cgttcaccaa agggctgcgt ctgggcaaca tgccgcagat ccgcgtgatt gtggatgaag
     7381 agctggagag cgtgtggacc ggtaagaaga caccacagca ggcgctggat accgccgttg
     7441 agcgtgggaa ccagttactg cgccgctttg agcagtctac gaagtcttaa ttctcttacc
     7501 ctcaccctaa ccctctccca aagggagagg ggaccgatcg gcgtggtatc caggtgccgt
     7561 gtcggtttgc aacttcgctc ggtgggaaag tatgcaccgc ctttctccct cgcccctttg
     7621 gggagagggc cggggtgagg ggaacacaac acgaagagtt taactatgtc atcatcccgt
     7681 ccggtgttcc gctcgcgctg gctgccctat ttgctggtcg cgccgcagct cattatcacc
     7741 gttatctttt ttatctggcc tgcgggcgaa gcgttgtggt actcgctgca aagcgtcgat
     7801 ccgtttggtt tctccagcca gtttgtcggc ctggataact tcgtcgcgct gtttcatgac
     7861 agctactatc tcgactcctt ctggacgacg ataaaattca gcacctttgt caccgtcagc
     7921 ggtttgctgg tgtcgctgtt ctttgcggcg ctggtggagt acatcgtgcg cggcagccgt
     7981 ttctatcaaa ccttaatgct gctgccttac gccgtggcac ccgccgttgc cgccgtattg
     8041 tggatcttcc tgtttaaccc tggtcgcggg ctgatcaccc attttctcgc tgagttcggc
     8101 tacgactgga accacgcgca aaacagcggt caggcgatgt ttctggtggt gtttgcctcg
     8161 gtgtggaagc aaattagcta caactttctg ttcttctatg ccgcgctgca atccattccc
     8221 cgttcgttta tcgaagccgc agccatcgac ggtgcaggcc cgattcgccg cttctttaag
     8281 attgcgctgc cgcttatcgc tccggtgagt ttcttcctgc tggtggtgaa cctggtgtac
     8341 gccttcttcg acaccttccc ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc
     8401 accacgacgc tgatttataa gatttaccgc gaaggcttta ccggactgga tctggcttcg
     8461 tctgccgcgc agtcggtggt gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc
     8521 cgctatgttg aaagcaaggt gcgttaccaa tgattgagaa ccgtccgtgg ctgacaatat
     8581 tcagccatac catgctgatc ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt
     8641 ttgtcgcggc gacgctggat aaacaggccg tctacgccgc gccgatgacg ctaatccccg
     8701 gcacacatct gctggaaaac atccacaaca tctgggtgaa cggggtaggc acgaatagtg
     8761 cgcctttctg gcggatgttg cttaacagct ttgtgatggc gttcagcatt acgctcggca
     8821 aaattaccgt ctcgatgctt tcggcatttg ccattgtctg gtttcgtttt ccgctacgta
     8881 acctcttctt ctggatgatt tttatcactc tgatgttgcc ggttgaagtg cgtattttcc
     8941 cgacggtgga agtgatcgcc aatctgaaaa tgctcgacag ctacgccggt ttaacgctgc
     9001 cgctgatggc ctcggcgacc gccacgtttc ttttccgcca gttctttatg acgctgccgg
     9061 atgagctggt ggaagcggcg cggatcgacg gtgcgtcacc aatgcgcttc ttttgcgaca
     9121 tcgtttttcc gctctccaaa actaatctgg cggcgctgtt tgtgatcacc tttatctacg
     9181 gctggaacca gtatttgtgg ccgttgttga ttattaccga tgtggatctc ggcaccaccg
     9241 tggcagggat taaagggatg atcgccactg gcgaaggcac cacggaatgg aactcggtga
     9301 tggcggcgat gttgttaacg ctgatccctc cggtggtgat tgttttagtg atgcagcgtg
     9361 cctttgtgcg cggcctggtc gatagtgaga aataagatgg caggactgaa attacaggca
     9421 gtaaccaaaa gctgggatgg caaaacccag gtcattaaac cgctgaccct tgatgtggcg
     9481 gatggcgaat ttatcgtgat ggtcgggccg tctggctgcg ggaaatcgac gttattgcga
     9541 atggtggccg gactggagcg ggtaacgacg ggcgatatct ggatcgaccg caagcgtgtc
     9601 accgaaatgg agccgaaaga tcgcgggatt gcgatggtgt tccagaacta cgcgctctat
     9661 ccgcatatga gcgtagagga gaatatggcg tgggggctga aaattcgcgg catgggcaag
     9721 cagcaaattg ccgaacgtgt taaagaagcg gcgcgcattc tggagctgga tggcctgctc
     9781 aaacgtcgcc cacgcgagct atccggcggt cagcgtcagc gtgtggcgat ggggcgtgcg
     9841 attgtgcgtg agccggcagt gttcctgttt gatgagccgc tctctaacct cgatgccaag
     9901 ttgcgtgtgc agatgcgtct tgaactgcaa cagctgcacc gtcgcctgaa aacgacttca
     9961 ctctacgtta cccacgatca ggttgaggcg atgacgctcg cccagcgagt aatggtgatg
    10021 aacggcggcg ttgccgaaca gattggcacg ccagttgaag tgtacgaaaa gcccgccagc
    10081 ctgtttgtgg cgagttttat cggcagtccg gcgatgaatc tgctggcagg gcgtgtgaat
    10141 aacgaaggca cgcatttcga actggacggc ggtatcgtgc taccgctaaa cggtggctac
    10201 cgtcagtatg ccgggcgtaa aatgactctc ggcattcgcc cggaacatat tgcgctaagc
    10261 tcgcaggcag aaggcggcgt accgctggtg atggacacgc tggagatcct cggcgctgat
    10321 aatctggcgc acggacgctg gggcgaacag aagctggtgg ttcggctggc gcatcaggag
    10381 cgcccgacag caggcagcac gctgtggctg catctgccag aaaatcagct gcatcttttt
    10441 gatggtgaaa caggacaacg agtatgagta actggcctta tccccgcatc gtcgctcatc
    10501 gtggtggcgg taagctggcc ccggaaaaca ccctggcggc aatcgacgtt ggggcaaaat
    10561 acggtcataa gatgatcgaa tttgacgcga agttatcgaa agatggcgag atcttcctgc
    10621 tccatgacga caatcttgaa cgtaccagca acggctgggg cgtcgcgggc gatctgaact
    10681 ggcaggattt actgcgcgtg gatgcaggca gttggtacag caaagcgttt aaaggtgagc
    10741 cgctaccgtt gctttcgcag gtggcggaac gctgtcgcga acacgggatg atggcgaata
    10801 tcgaaatcaa acccaccact ggcaccgggc cattaacggg caaaatggtg gcgctggcgg
    10861 cacgcgaact gtgggccgat atgacgccgc cgctgctgtc atcgtttgag attgatgctt
    10921 tagaagctgc acaacaggct gcaccggaac tgccgcgcgg tttgttgttg gatgagtggc
    10981 gcgacgactg gcgagaactg accgcgcgac tgggctgcgt ctctattcat ctcaatcata
    11041 agttgcttga taaagcgcga gtgatgcagt tgaaagacgc cggattacgg attctggttt
    11101 ataccgtcaa taaaccccag cgcgcggcag agttgctgcg ctggggcgtg gattgcatct
    11161 gcaccgatgc gattgacgtg attggtccga actttacggc ccaatagttt tcaacggaat
    11221 atcaggctgc ggcgtgctgg gctgattcag catgtcgccg tttctcctct ccggcaacat
    11281 atgctgctga ttcaacgaac tatccggatt acggttgctg tttaacatcc cgccgttggt
    11341 gttgggcagc atttgctgcc gggcgggatt tcgctccccc ggctgtgact gcaacacccg
    11401 ctgagaatta ttgttgatct ggttttctaa atgctgttgt tgcagctgcg tttgcgtttt
    11461 cagttgctga ttcagcatcc ctttttgctg gatttgctgc gtttgcatct gggtttgcat
    11521 ccgctgctga ctggggatct gataccccgg ctggttaggg ttgttcagag tattaatggg
    11581 ctgtgcaaag ccgacaaacg gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta
    11641 tcctcctctg aagatattct ttaagtttac tcctttcctg caatcatcag gattaattca
    11701 gagttatata ccaggcttag ctggggttgc cccttaatct ctggagaata acgatgataa
    11761 aaccgacgtt tttacgccgg gtggccattg ctgctctgct ctcaggaagt tgttttagcg
    11821 ccgccgccgc gcctcctccg ccacctgttt cttatggcgt ggaggaagat gtcttccatc
    11881 cggtacgcgc gaaacagggg atggtggcgt ctgtagatgc tactgccact caggtggggg
    11941 tggatatcct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg ggctacgcgc
    12001 tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg ctgatccgct
    12061 cgaaaaatgg caataccacc gctatcgatt tccgcgaaat ggcacccgcc aaagcgaccc
    12121 gcgatatgtt cctcgatgac cagggcaacc cggacagcaa aaaatcactc acttcgcatc
    12181 tggcctccgg tacgcccggt acggtagcag gtttctcgct ggcgttggat aaatacggca
    12241 ccatgccgtt gaacaaagtg gtgcaaccgg cgtttaaact ggcacgcgat ggttttatcg
    12301 ttaacgacgc gttggctgac gatctcaaaa cctacggtag cgaagtgttg ccgaatcacg
    12361 aaaacagcaa ggctatcttc tggaaagagg gcgcgccgct gaaaaagggc gacaagctgg
    12421 tgcaggcgaa cctggcaaag agcctggaga tgattgccga aaacggcccg gacgaattct
    12481 acaaaggcac gatagcggaa cagattgccc aggagatgca gaaaaacggt ggcttgatca
    12541 ctaaagaaga tttagcggcg tataaagcgg tcgaacgtac accgattagc ggcgattatc
    12601 gcgggtatca ggtttactcc atgccaccgc catcttccgg cgggatccat atcgtacaaa
    12661 tcctcaatat tctggaaaac ttcgatatga agaaatacgg ctttggcagc gccgacgcga
    12721 tgcaaatcat ggcagaagct gagaaatatg cctacgccga ccgctcggaa tatcttggcg
    12781 acccggattt tgtcaaagtt ccgtggcagg cgctgaccaa taaagcctat gccaaatcta
    12841 ttgccgatca aatcgatatc aataaagcga agccatccag cgaaattcgc cccggcaagc
    12901 ttgcgcctta tgagagtaat caaactaccc attactcagt ggtggataaa gacggcaacg
    12961 cggtagcggt gacctatacg ctgaacacca ccttcgggac gggcattgtc gcgggcgaga
    13021 gcggtattct gcttaataac cagatggatg atttctccgc caaacctggc gtaccgaacg
    13081 tttacgggct ggtgggtggt gacgccaacg cggtcgggcc gaataaacgc ccgctgtcgt
    13141 cgatgtcccc aaccattgtg gtgaaagacg gtaaaacctg gttagtgact ggtagcccag
    13201 gcggtagccg gatcatcact accgtgctgc aaatggtggt gaacagcatc gattatggta
    13261 tgaatgtcgc cgaagcgacc aatgcaccgc gtttccacca tcagtggttg ccggacgagc
    13321 tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca aaaggtcaga
    13381 aagtggcgct gaaagaggcg atgggtagta cacaaagcat tatggttggg ccggacggcg
    13441 agttgtacgg cgcatccgat ccgcgctcgg tggatgattt aacggcgggt tattaaggtt
    13501 agcggccctc ttcgtgggaa gagggccgta ttgtcagggc aagccgaagg tagccttttt
    13561 tatttcataa acctgtagat attcttccag cggctttgcc gttccaaggg aatgaatttc
    13621 accactgtct ttatcaataa taaaaggtgc gttaccggct aaacgcgcgg cctcatctcc
    13681 agattcaaga aattcttgtg attcgaaaca gaaataccag ccctggctaa a
//
LOCUS       DARNVM010000128        13724 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.128, whole genome shotgun sequence.
ACCESSION   DARNVM010000128 DARNVM010000000
VERSION     DARNVM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.128"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1115
                     /gene="hcp"
                     /locus_tag="SI621_003445"
     CDS             <1..1115
                     /gene="hcp"
                     /locus_tag="SI621_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEI4066114.1"
                     /translation="NITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSNSITNT
                     GKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNIQLRGA
                     LLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSYLAFPDNYNRLFIPRPKTKD
                     DNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNKGLLIAGA
                     SIVAGVVLGRMRFPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTIDGVTINREGIA
                     IVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYCNLGWETG
                     EPKGLDGYELWNNVHTATLEDFKIKDTDLFHPDATK"
     gene            1112..1369
                     /locus_tag="SI621_003446"
     CDS             1112..1369
                     /locus_tag="SI621_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEI4066115.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSRPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(1858..4119)
                     /gene="metE"
                     /locus_tag="SI621_003447"
     CDS             complement(1858..4119)
                     /gene="metE"
                     /locus_tag="SI621_003447"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEI4066116.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPIYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRGDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4237..5190
                     /gene="metR"
                     /locus_tag="SI621_003448"
     CDS             4237..5190
                     /gene="metR"
                     /locus_tag="SI621_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HEI4066117.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5078..5977)
                     /gene="bioP"
                     /locus_tag="SI621_003449"
     CDS             complement(5078..5977)
                     /gene="bioP"
                     /locus_tag="SI621_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HEI4066118.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6053..6853)
                     /gene="yigL"
                     /locus_tag="SI621_003450"
     CDS             complement(6053..6853)
                     /gene="yigL"
                     /locus_tag="SI621_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HEI4066119.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(6861..7883)
                     /gene="pldB"
                     /locus_tag="SI621_003451"
     CDS             complement(6861..7883)
                     /gene="pldB"
                     /locus_tag="SI621_003451"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HEI4066120.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7994..8614
                     /gene="rhtB"
                     /locus_tag="SI621_003452"
     CDS             7994..8614
                     /gene="rhtB"
                     /locus_tag="SI621_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HEI4066121.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8676..9296)
                     /gene="rhtC"
                     /locus_tag="SI621_003453"
     CDS             complement(8676..9296)
                     /gene="rhtC"
                     /locus_tag="SI621_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HEI4066122.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9360..11189)
                     /gene="recQ"
                     /locus_tag="SI621_003454"
     CDS             complement(9360..11189)
                     /gene="recQ"
                     /locus_tag="SI621_003454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEI4066123.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGIGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11322..12191)
                     /gene="pldA"
                     /locus_tag="SI621_003455"
     CDS             complement(11322..12191)
                     /gene="pldA"
                     /locus_tag="SI621_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEI4066124.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12356..12823
                     /locus_tag="SI621_003456"
     CDS             12356..12823
                     /locus_tag="SI621_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEI4066125.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12875..>13724
                     /gene="rarD"
                     /locus_tag="SI621_003457"
     CDS             12875..>13724
                     /gene="rarD"
                     /locus_tag="SI621_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEI4066126.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPISLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIF"
BASE COUNT         3423 a         3584 c         3429 g         3288 t
ORIGIN      
        1 tgaacataac cggagaacaa caaggatgta tttcctctcg ttgtggaacg agcgcatcaa
       61 taggtaatcg ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata
      121 gtattacgaa tacaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattgattg
      181 acaaaagctc cccgttactt attaacgcta ttaacaataa cgagcagttg ttcatggagt
      241 ttgacttcta tcggatcaat agatttggtc gatgggagaa atattacaac attcaacttc
      301 gcggcgcgct cttatcagca atcaatcacc tgtttactga gaacaatctg gatacagaaa
      361 ccatcactgt cagttatgaa tatatactct ccaggcatct gattgctaat actgaattta
      421 gttatttggc atttcccgat aactacaacc gtctgtttat accacggcca aaaactaaag
      481 atgataatgg cctcaaaaca ctcaatagta aagcggttgg cagactgctt gctgctggcg
      541 gcatatacaa tgggaatatc gaaggattca gggaaacagc aaacaagttg ggaggagatg
      601 cacctgctgg ttacgatcaa gttatggata acaaagggtt actcatcgct ggtgcttcaa
      661 tagttgctgg agttgttctg ggaagaatga gattccctga tttggaatca cttgagcatt
      721 tcggagccag aggagcagta tcaggaagac catttaatcc cgaccttgcc ggtggtccaa
      781 ttgaaaacct gacgatagat ggcgtaacaa tcaacaggga aggtatcgct attgttgaaa
      841 agcatatctc cagatttgat catgatccag ctaatgatgt aatgatttca aggctaaaga
      901 aaatagcaaa taaagaattg cttcctgaaa aatacgatct gaactactat actcatgaat
      961 gtcgcgagta tcagcgttat tgtaatttag gatgggaaac aggtgaaccg aaaggtttag
     1021 atggatatga attatggaat aatgtacata ctgcaactct agaagacttt aaaattaaag
     1081 atactgactt atttcatccg gatgcaacaa aatgacgatt acgatagttg aatttaacgt
     1141 gctgaaagtt atggctgaga aagatattga ctggagctgg atggtgctgg atcgtacttt
     1201 agctatacga aatattccag gatttggcaa cgtagcgaat atcgtaacaa agctggttaa
     1261 tcatgggttg gttgatatcg taaatggtga gggcaactct agaccacgct acagagtttc
     1321 tcaatatgga ttgaatctta tcaaagaaca acaagacaac ctcttctgat ccagccatta
     1381 caggagcatg atttacattg tgatctttca ataagatact catcaatttt ggtacagaat
     1441 atcgtgatca ctaaaaatga acttaacgtg ctgaacgtta tgactgagaa agatataaac
     1501 tggaactgga tgaatctcga tcgcacacta tccataaggc aaattccagg attcgggaat
     1561 gttgttaaca ttgttaacaa attagctaac gaaggtttgg ttattattga ggatagcggg
     1621 aataagtcta tgcctcacta ccacgttagt gaaaagggat acaatctcgt gcaacgtgag
     1681 aataaataac gtagcgttga ttactgaacg gaatcactca aaactgtgtt ttgcgaaaaa
     1741 aacgattcgg ttatttctgc tcctgaagat cagcttcaat ctggcactca tgcaaaatgg
     1801 cttccagtgc tggaagccat tatcgtactg caatttacag tgactcgtta atattcgtca
     1861 tccccgacgc aaattctgcg ccgcctgcac catgttcgcc agtgccgcgc gggtttctgg
     1921 ccagccgcgc gttttcaggc cacagtccgg gttgacccac agacgctctg ccggaatgcg
     1981 ttttgccgct ttcttcagca aggcttcaat ccattctacg ctcggtacgt ttggcgagtg
     2041 aatgtcatag acgccaggac cgatttcatt tggataatca aactcttcaa acgactccag
     2101 caactccatg tcggaacgcg aggtttcgat ggtgatgacg tctgcgtcca gcgccgcaat
     2161 cgaatccatg atgtcgttga actcgcagta acacatgtga gtgtggattt gtgtgtcatc
     2221 tttcgccacg gcggcgttga tacggaaagc ctccacgccc cactggagat aggcatccca
     2281 gtcgccgcga cgcagcggta aaccttcgcg cagtgccggt tcatcaatct ggatgatgcc
     2341 aattccagcg gcttccaggt ccgccacttc atcacgcagc gccagcgcaa tttgtttggc
     2401 gatggtttca cggctgacat cttcacgcgg gaacgaccag cagagaatag tcaccgggcc
     2461 ggtcaacatc cctttcaccg gtttatcagt cagggattgc gcatacttcg cccactcaac
     2521 ggtaatcggt gccgggcggc taacgtcacc aataacaatc ggtggcttca cgcagcgtga
     2581 accgtagctc tgtacccaac cgttttgcgt aaagacaaaa ccgtcgaggt gctcgccaaa
     2641 gtattccacc atgtcattac gctcagcctc gccatgtacc agcacatcca gtcccaaacg
     2701 ttcctgctca acaatggcct gcttgatatg ttccgcaatg cccgtgcggt agttattggc
     2761 gtcgagatta ccctttttaa aatccagacg cagggtacga atctccgtgg tttgcgggaa
     2821 ggaaccaatc gtggtggtcg gccacgcggg cagtttaaaa cgcgcacgct gggcttcagc
     2881 acgcacttca tagacattcg cacgctgact gtcctgggcg gtgatcgccg ccagacgctt
     2941 ttctactgcc ggattatgaa cacgagtaga gtgacgacgc gcctgaatcg gagcgctcca
     3001 ctctgccaga gcagccgtgt caccactgtt cagcgcatcg cgcagcagtg ccagttcatg
     3061 acatttttgc agggcgaagg caaaccagct tttcacttct gcatcaagac gcgtttccac
     3121 gctcaggtcg atggggctgt gcagcagtga gcaggaagat gccacccaca aatcgcgttt
     3181 gccgacaatg tccttaattt gcgcatactt ctcggtaaga tcggcgcgcc agacgttacg
     3241 accattgatc agacccgcag acagcagcca gtcagaaggc agacgcttgt gcagttcagc
     3301 aacgtcatct ttaccatgta caagatcgac atgcagaccc tgaacaggca gcgcagtaat
     3361 cgtgtcgaga tttggcgtta cgccttcaaa ataggtggtc agcagcagtt tcacctgtcc
     3421 ctggagcgcg tcgtaagcgg gtttgtatgc gtccagccac gcctgcggca gttccagtac
     3481 cagcgcgggt tcatcaatct gtacccactc aatgccgcgt tttgccagtt ctgccagcac
     3541 ttgctgataa atcggaagaa tgtcgttcag caggctcagg cgatcaaact gttcaccttt
     3601 cactttaccc agccacaggt aggtaaccgg ccccagcagc acaggtttca ccttgtggcc
     3661 cagcgccagc gcctcgtcca cttcgtccag cagctgcgtc caggtcagtt tgaactgttg
     3721 gcctttaacg aattccggca ccatgtagtg atagttggta ttaaaccatt tggtcatttc
     3781 cgctgccgcc gcaggttcgc cagtcggcgc acgtccacga ccaatacgga acagggtgtc
     3841 gatatctacc gaaccatctt tgttctgatg acgcgccgga acgttaccca gcagcagact
     3901 ggtggtcagt acatgatcgt accaggcaaa atcgcccacc ggcagcaggt cgatacccgc
     3961 ttgcttttgt tgatcccagt gacgagcacg caattcacgc cctaccgcca gcagttcttc
     4021 acgcgtggag ttccccgccc aataactctc ttgcgctttt ttcagctcgc gacgcaggcc
     4081 aacgcgaggg aaaccgaggg tgtgattaag aattgtcatt atatgcccct tatggaattt
     4141 ttagtattta gccgtccaga tgtttacaca tccataattg gcggttactg tatattcctc
     4201 aagcgcaaat tattcatgcc gaagtgaagg actttcatga tcgaagtaaa acatctgaaa
     4261 acgctacaag cgttgcggaa ctgcggctcg ctcgcagccg ctgcggcgac gttgcatcag
     4321 acgcaatccg ccctgtctca ccagtttagc gatctggaac aacgccttgg cttccggcta
     4381 tttgtgcgta agagccagcc gcttcgcttt acaccgcagg gagaaatcct gttgcaactg
     4441 gcaaaccagg tgctgccaca aattagccag gcactgcaag cctgcaatga accgcagcag
     4501 acgcgtctgc gcatcgccat tgagtgccat agctgtattc agtggctgac gcccgcgtta
     4561 gaaaatttcc ataagaactg gccgcaggtc gagatggact ttaaatcggg cgtgacgttt
     4621 gacccgcagc ctgcgttgca acagggcgag ctggatctgg tgatgacgtc agatattctg
     4681 ccgcgcagtg gcctgcatta ttcgccgatg ttcgattatg aagtgcgtct ggtgttggca
     4741 cctgaccatc cgctggcggc aaaaacgcgt attacaccgg aagatctcgc cagcgagacg
     4801 ctgttgattt atccggtgca gcgtagccga ctggatgtct ggcggcattt tcttcagccg
     4861 gcaggcgtca gcccgtcact gaaaagcgtc gataacacct tattgttgat tcagatggtt
     4921 gccgcgcgga tgggtattgc cgccctgccg cattgggtag tagagagttt tgagcgccag
     4981 ggtctggtgg tgaccaaaac cctgggcgaa ggcttgtgga gccgactgta cgccgccgtg
     5041 cgcgatggcg agcagcgtca gccgattact gaagcgttta ttcgctcagc gcgcaatcac
     5101 gcctgcgatc atctgccgtt tgtgaagagc gcggagcgac ccacttacga tgcacccaca
     5161 gtgaggccag gatcaccagc gcgcctgtaa tgaacgttgg ccagtgcggc tgttggtgcc
     5221 agatagccag gtttaccagc agccctgccg gaacgtgcat attattcata atacccagcg
     5281 ttccggcgtc tacttgggtt gcgccgtagt tccacataaa gtagccaatc ccggaagcca
     5341 ccacgccaag aaacaccaga atgccccatt gcaacgtggt ttgtggcatt ttctgcgcat
     5401 ttcccagcaa gaaccatgca atcaccgcca ccaggaacgc gccaagatag aaccacgcaa
     5461 aggcgttatg ctgcggcatc gggcgagttt ccatcagacg tttgtaaccc accatgccga
     5521 tggcaaaagt gatattggag agctgcacca gcagcaggcc aatccagaag tggtcggtga
     5581 cctgatcata gcgaataatc ccggcaccaa tcaccgccaa caaggcgcta aaagcatagc
     5641 cccagcgcag acggcgctta ctcatgatgt cataaatcaa cgtaatgtag agcggcgtca
     5701 gtacggtgaa cagcaacaat tcggagaccg tcaggtagag ataggcgcga aaactcagca
     5761 tatacatcac gccgagctgc atcgcgccca ccagcatata caggccgacc gttttcaggc
     5821 tattgccacg ggtacgcaga aacggcagaa aaacgagtgc cgccaggcca acgcgcacca
     5881 gcaccgcaaa atagctatcg acgtgccccg caaggtactc gccataaaag ctaaaggaga
     5941 aggcccacag aatcgtggtg atgataagta gcgccacaat ggaaaactct ttgattaacg
     6001 agtatccatt gtagcgaagt atcaggttga caactggcca aataaagaac gattacgata
     6061 aatagagctt acgcagataa tgcggcaccg cgtcgtcggc attagtacca atcacttcca
     6121 gctccggatg cagatctttc agacgctggt gcgcactgcc cataatgcag cctttccccg
     6181 ccatcgacag catttcggcg tcgttcatcc cgtcaccaaa ggcaatacaa tccttcaggc
     6241 tgtagcccag tttcttcgcc accgcttcca gcgcatgacc tttcgacacg ccgcccgcca
     6301 tcacttccag acaggttaag gtagagaaac taacgttgac gcgatcgccc cagcgagcgt
     6361 tgatcgcctg ctccagcggc agaagttgtt catgggaatc gcaggtgaag aacactttgc
     6421 tgacgccttc cggctccagt aatcccggct catacagcgc atatttgaac accgcttctt
     6481 taaaaaagcg catctcttcc gggcgatggc gattcataaa ccattcgtcg tcgcgataaa
     6541 cgttagtaat gatgtccgga ttgtcgttga ctacgccaaa cagatcgctg gcaatgtcgc
     6601 gatccaggtt atgagcaaaa atcagattgc catccagatc gtgaacgcgc gcgccattgg
     6661 aggtaatcat gtaagactta atctccagat tatcgcgaat ttgccccaca tcaacgtggt
     6721 gacgaccggt cgcaaacaca aagttgatgc cgcgcgcggt gagcagcttc agagtttctt
     6781 tggcgtaagg ggataacgta tggtcgggag aaagtaacgt gccatctaaa tcagacgcaa
     6841 caacctggta cataagaaat ttaaacctct gtagagcggt ttccgctggg tgagttatgc
     6901 ctgttgaaaa aatcaacgat ggcgtggagc gcgactgagc gcattgcgtc cttttcaaaa
     6961 aggatctcat ggtaagcacc tttaattacc aacggccgtc ctccttcgac aggatggccc
     7021 gcggcggtgc ggagttcaca aaaacggtcg tgcatgcggt tatccaccac gcgttcctct
     7081 tcagcttgta aaagaagcgt tggcgtggcg tcatcacccg caccggctaa cacctgttcg
     7141 ccagccagaa tactttcgcg tacccaatgg taggttggcc caccgacgcg aatcgttggg
     7201 tcatcagcat agaagcgtaa gttacgtcga tatcgctgtc tgctgtgggt cagtacgttg
     7261 atagcaaacg gcaacgcgcg ccagcgcccg gtgcctattg catagccatc acggaaacgt
     7321 ggatgcgctt cggcccaatt gaggatctgc cgtgccataa atgacggcat acgaatcacg
     7381 atcccaaaca ttggcgcagt tagcgcaata gcgtcacata cacctggatg gcgttgcaga
     7441 aataatgtgg agatcgcacc gcccatcgaa tgtgccagta tatagcgttt acgccacgga
     7501 ccgggctgga cctcctgttg ccagaatgcc gccagatcat caacataatc attaaagcga
     7561 ttaacatgcc cgagatgcgg atcggctaac aggcgaccgg aacgtccctg cccgcgatgg
     7621 tcgatgatta agacatcaaa ccccaaatgg aacaggtcgt aggccagttc cgcatatttt
     7681 acgtagctct caatacgccc cgggcagatg actaccaccc ggtcatggtg ctgtgcgcga
     7741 aaacggacaa agcgcaccgg aatgtcatcc acaccagtaa actctgcttc atctcgctga
     7801 cgccagaaat cagtcagcgg tcccatggta aaagcagcaa acgcgttttc tcttgtttcc
     7861 cagtcttttt gctgctgaaa catcgggtaa tctgcctctt aaaccacgta aaatcgtttt
     7921 tttaagcgtg cctgacacaa cgctgcgaca gtagcgtatt gtggcacaaa aatagacaca
     7981 ccgggagttc atcatgacct tagaatggtg gtttgcctac ctgctgacat cgatcatttt
     8041 aagcctgtcg ccaggctctg gcgcaatcaa caccatgacc acctcgctca accacggtta
     8101 tcgcggcgcg gtggcgtcta ttgctgggct tcagaccgga ctggcgattc atattgtgct
     8161 ggttggcgtg gggttgggga cgctattttc ccgctcagtg attgcgtttg aagtgttgaa
     8221 gtgggcaggc gcagcttact tgatttggct gggaatacag caatggcgcg ccgctggtgc
     8281 aattgacctt aaatcgctgg cctctactca atcgcgtcga catttgttcc agcgcgcagt
     8341 ttttgtgaat cttaccaatc ccaaaagtat tgtgtttctg gcggcgctat ttccgcaatt
     8401 catcatgccg caacagccgc agctgatgca gtatatcgtg ctcggcgtca cgactattgt
     8461 ggtcgatatt attgtgatga tcggttacgc cacccttgct caacggattg ctctatggat
     8521 taaaggacca aagcagatga aggcgctgaa taagattttc ggctcgttgt ttatgctggt
     8581 gggggcgctg ttagcatcgg cgaggcatgc gtgaaaaata atgtcggatg cggcgtaaac
     8641 gccttatccg acttactctg atgacgcgtc tggcatcaac gcgaaataat caaatgaatg
     8701 ccaaatccgg caaataacgc cccggcaaaa ccatcaatcc acttcgccag acgttgataa
     8761 ccacggcgca tttgcggcag ggcaaacagg ctggcaacga cggtaaacca cgccagtgtt
     8821 tcgataataa tcaacgcgaa aatgccccag cgctcagtgg tgccgacgtt atcgccgaca
     8881 aacagcgaga ataccgaacc aaagtagata atcgctttcg gattggcgag attggtcagt
     8941 aagcctttca ggaaactgcg tccgcttttt gccagttcta cctgtggcgc aggcgcagaa
     9001 acgacctctt ttttcagtgc gctacgcagc atctgataac ccatccagca gagatacagg
     9061 ccaccgccca ccataatcag cgtatgcagc caggccattt tttcgataat caaatgtagg
     9121 ccaagcaacg caatcccagc ccaaaccatt acgccgcagg taatgcccag cacgcccatc
     9181 atcgcttctt tacgggaacg actaacagcg gtctgagaga caaaaaagaa atcgggaccg
     9241 gggctcataa gcgccacaat gtgcaccatg gcgacggtga ggaataacat caacatacat
     9301 tagactcgcg ggggaaacgt ttacggagtc ttcatcctgg cactttttta tgctgctgac
     9361 tactcttcgt catcgccatc aacatgcgca cgaatcagcg ccataaacgg tttgccaaag
     9421 cgttccagct tgcgcatccc aatgccgtta acgctgagca tttcgctggc agtgatcggc
     9481 atctgttccg ccatctcaat caaggtcgcg tcgttaaaca ccacgtacgg cgggacattg
     9541 ctttcatcgg caatcgattt acgcagtttg cgtaatttgg cgaacagttt gcgatcgtag
     9601 ttgccgccga aggatttctg catcgctttc ggtttgagcg ccacgatacg tggcacggca
     9661 agttgcaaag aggattcgcc gcgcagcacc ggacgcgcag cctctgtcag ttgtagggca
     9721 gaatgctggg caatattttg cgtcaccagg ccgaggtgaa tcagctggcg gatcacgctc
     9781 acccaatgtt catggctttt atcacggccc atgccataga ctttcagttt gtcatgacca
     9841 tagtcgcgga tacgctggtt attagcgcca cgaatcactt ccaccacata acccatccca
     9901 aaccgctgat tcacacgacc aatggtggaa agggcaatct gagcatcggt tgaaccatcg
     9961 tattgtttcg gcggatcgag gcagatatcg cagttcccgc acggctcctg ccgcccttcg
    10021 ccaaaatagt ttagcagcac cagacggcga caggtttgcg cttcggcaaa cgcgcccatc
    10081 gcattgagtt tatgacgttc gatatcctgt aactgcccct gcggtttctc ttccagacaa
    10141 cgacgcagcc acgccatatc cgccggatcg taaaacagca tcgcttccgc gggcaggcca
    10201 tcacgcccgg cgcgtccggt ttcctgataa taggattcaa tgttgcgcgg aatatcaaaa
    10261 tgcaccacga agcgaacgtt tggtttattg atgcccatgc cgaacgccac cgtcgccacc
    10321 acaatttgca ggtcatcgcg ctggaatttc tcctgcacat cagcgcgaac attattttcc
    10381 agcccggcat gataggccgc cgcgctaatt cccttgcttt gcaggcgcgc agcggtgtct
    10441 tctactttcg cacgactatt acagtaaata atccccgact taccgcgttg ttcctgtaca
    10501 taacgcatca actgatcgag cggtttgaat ttctccatca gcatgtagcg aatattcgga
    10561 cggtcaaaac tgctgatttg aatcagcgga tcgttcagcc ccagcaggcg cacgatatcc
    10621 tggcgcgtgg tgtcatcggc tgtggcggtc agcgccataa acggcagcgt cgggaaccgc
    10681 tggcgcaact gaccgagcgc ggcatattcc gggcggaaat cgtggcccca ttgggagata
    10741 cagtgcgctt catcaacggc taataacacc ggattccagt gcgccagatg ctcaagaaag
    10801 ttatccagca tcaggcgttc cggcgcgata taaagcaggc ggatttgccc ggtgcgacag
    10861 cctgtcataa cttcaagttg ctgctcgcgg gtttgcgtcg agttaaggca cgccgccgcc
    10921 acgccgttgg cttgcagttg atccacctga tctttcatca acgaaatcag cggtgaaaca
    10981 accacggtaa ggccgtttag caataaagca ggaatttgat agcaaaggga ttttccgcca
    11041 ccagtgggca tgacgaccag gcaatcgcga ccggaaagca cagtgtcgat aatctcttcc
    11101 tggccggggc gaaactgttg gtagccaaag gtttcttgta aaacctgttt agctccggac
    11161 tccagattca acacttccgc ctgcgccaca ttcaccccga ttgcctgaaa taaaaacagg
    11221 cgctattttc agcgcctgcg cgagaaactg caatgctttt agtctctgag agggtaatcc
    11281 tggatactgg attccagatt tgccaaccac tcaaccgttc atcaaaacaa atcgtttagc
    11341 ataaccccca caccgacacg ggtctggttg aagttatagt cgatgagcga ttcgccatag
    11401 ccgctgtaaa cctgagtata aaggcgcaca tgtttggtga tcgggtaact taagcctaac
    11461 tccgcgccgc cgtagccggt gttccagttg tactgtcctt tcgcactcaa caccgcatca
    11521 ccgaggtgat agccgatttt aagctggtaa tagcccatat atttggtgat gtccgggtta
    11581 tcgtccgtat tccccaccac ataccacggc ttcacctcta ccagccagtt accgttttct
    11641 gccatcaggc gggtataaag gcggttccag ctgcgggaag tcgggtctga acgcccgttt
    11701 gagtcgtggt tgtagcccat ttccacatcg cgcagcgtcc agccagcaaa gttgtaatcc
    11761 gtggcaaaac cgaggaacaa ttgcggttcg tagttagttt cacgaaacgg tgaagactct
    11821 tcgctattgg acagttgcca ccaggatttt tgcgtataag acgcacccaa caccgagttc
    11881 gggcctaaaa tcccacgcca cagcggaaac gccaggctca actgaaactt tacttcatcc
    11941 ttacgcgcat tttccgccca gtcgtaactg gcaatcgctt ctttattcag atcgctggtt
    12001 tgggtataga tgatgtagtt ggtgtcataa ggataaaggg tgaacggatt gtcatgctcc
    12061 tgcagcatat tggcgataat actgccacgc accgctggcg cgtcatgcac ctctttcacc
    12121 gttgcctctt gtgcatatac tgccataggc aacataaaca ccggcaacaa ccagccctgc
    12181 agagtccgca tcgctcgtgt tctccgtaac gaattattga taaggtgaat atttttctgc
    12241 gggctatttt acatacttaa ctccatagcc gttagttatc ccttcctaaa gtagaaacca
    12301 acaacaaaga agcataaaat caacatttca ttaactaatg aagtgtaagg atgatatgtc
    12361 tgccgtactg accgctgaac aagccctgaa attagtgggt gagatgtttg tttatcacat
    12421 gccatttaac cgcgcattgg ggatggaact ggagcgttac gaaaaagagt tcgcacagct
    12481 ggcctttaaa aatcagccaa tgatggtggg taactgggcg caaagcattt tgcacggcgg
    12541 ggtcattgcg tcggcgctgg atgtcgccgc cggtctggtg tgcgtgggaa gtaccttaac
    12601 ccgccacgaa accatcagtg aagatgaact acgtcagcgg ctatcgcgaa tggggaccat
    12661 tgatcttcgc gtcgattatc tgcgcccagg cagaggcgag cgttttactg ctaccagtag
    12721 cctgttgcgg gcaggcaata aagtcgccgt cgcccgcgtt gaattacaca atgaagaaca
    12781 gctttatatt gccagcgcta cagccaccta tatggtaggt tgataacgta aaagcaggta
    12841 aactgctgtt accaattaaa aatgagtatt cccgatggat gcaaaacaaa cgcggcaggg
    12901 cgtattactc gctcttgccg cttattttat ttggggtatt gcgccagcgt acttcaagct
    12961 gatttactac gtgcccgccg atgagatcct gacgcatcgc gtgatctggt cgtttttctt
    13021 tatggtggtg ctgatgagca tctgccgcca gtggtcctat ttaaaaacgc tgattcagac
    13081 gccacagaaa atttttatgc tggcagtctc tgccgtgctg attggtggca actggctact
    13141 gtttatctgg gcggtgaaca atcaccatat gctggaagcg agccttggtt actttattaa
    13201 cccgctggtg aacattgtgc tggggatgat tttcctcggc gagcgattcc gccggatgca
    13261 atggctggcg gtgattctgg cgatatgtgg cgtgttggtt cagttgtgga cttttggttc
    13321 actaccgatt atcgcgctgg ggctggcgtt tagttttgcc ttctacggcc tggtacgcaa
    13381 gaagattgct gttgaagcgc aaaccggcat gttaatcgaa actatgtggt tgctgcccgt
    13441 ggcggcgatt tacctgtttg ctattgccga cagctcaacc agccatatgg ggcaaaaccc
    13501 gatttcactg aatttactgc tgatcgccgc cgggattgtc actaccgtac cgctgttgtg
    13561 ttttactgcg gctgcgacac gcctgcgtct ctcaacgtta ggcttttttc agtacattgg
    13621 cccgacgctg atgttcctgc tggctgtgac gttttatggt gaaaaaccgg gtgccgataa
    13681 gatggtgacc ttcgccttta tttgggtggc gctggcaatt tttg
//
LOCUS       DARNVM010000129        13705 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.129, whole genome shotgun sequence.
ACCESSION   DARNVM010000129 DARNVM010000000
VERSION     DARNVM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.129"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            287..964
                     /locus_tag="SI621_003458"
     CDS             287..964
                     /locus_tag="SI621_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HEI4066127.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLEKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            1036..1320
                     /gene="ppnP"
                     /locus_tag="SI621_003459"
     CDS             1036..1320
                     /gene="ppnP"
                     /locus_tag="SI621_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEI4066128.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            1468..1806
                     /locus_tag="SI621_003460"
                     /pseudo
     CDS             1468..1806
                     /locus_tag="SI621_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            1803..1886
                     /locus_tag="SI621_003461"
     CDS             1803..1886
                     /locus_tag="SI621_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066129.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(1964..2875)
                     /gene="rdgC"
                     /locus_tag="SI621_003462"
     CDS             complement(1964..2875)
                     /gene="rdgC"
                     /locus_tag="SI621_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEI4066130.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3000..3908
                     /gene="mak"
                     /locus_tag="SI621_003463"
     CDS             3000..3908
                     /gene="mak"
                     /locus_tag="SI621_003463"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HEI4066131.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGLRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYHRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4051..5235)
                     /gene="araJ"
                     /locus_tag="SI621_003464"
     CDS             complement(4051..5235)
                     /gene="araJ"
                     /locus_tag="SI621_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HEI4066132.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     VG"
     gene            complement(5361..8504)
                     /gene="sbcC"
                     /locus_tag="SI621_003465"
     CDS             complement(5361..8504)
                     /gene="sbcC"
                     /locus_tag="SI621_003465"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HEI4066133.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAVEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIHYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALQSQQQTLQQQE
                     ALAAQSLQKAQAQFDTALQDSIFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESMFAVK"
     gene            complement(8501..9703)
                     /gene="sbcD"
                     /locus_tag="SI621_003466"
     CDS             complement(8501..9703)
                     /gene="sbcD"
                     /locus_tag="SI621_003466"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEI4066134.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGAQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            9893..10582
                     /gene="phoB"
                     /locus_tag="SI621_003467"
     CDS             9893..10582
                     /gene="phoB"
                     /locus_tag="SI621_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HEI4066135.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            10640..11935
                     /gene="phoR"
                     /locus_tag="SI621_003468"
     CDS             10640..11935
                     /gene="phoR"
                     /locus_tag="SI621_003468"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEI4066136.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            12342..13661
                     /gene="brnQ"
                     /locus_tag="SI621_003469"
     CDS             12342..13661
                     /gene="brnQ"
                     /locus_tag="SI621_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HEI4066137.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
BASE COUNT         3085 a         3482 c         3632 g         3506 t
ORIGIN      
        1 aacgctatga ggaccctgac aaagagtgac cgcatcagac tgctcggaag ggattctgag
       61 tgccgccaca agggatctgc gtcacatttt tcacaattca tgtttttcaa ataattagaa
      121 tattaaacaa taacaatcca ttactggaat catttggaat ctttacatta tgccgtgcag
      181 gtctgctgct acgctttttg tcatttgtag cacaggtaag tgtcagcagt ggtgcttagc
      241 gcttgcccgg taattaacga cgaaataaaa gtaaggtgga agaacaatga gtgcgtcgtt
      301 ggcgatcctg accatcggca ttgtacctat gcaggaagtt ttgccgctcc tgacagaata
      361 cattgacgaa gataatattt cccatcatag cctgctgggg aagttaagcc gcgaagaagt
      421 gatggcggag tacgcgccag aagcaggcga agacacaatt ctcacattat taaatgacaa
      481 ccagctggcc catgtttcgc gtcgcaaagt ggagcgtgac ctgcaaggtg tggttgaagt
      541 gctcgataat cagggttatg acgtcatttt attaatgagt acagcaaaca ttagtagtat
      601 gactgcgcgt aatacgatct ttcttgagcc gtcgcgaata ttgcctccgc tggtttcttc
      661 tattgttgaa gatcatcagg tgggggttat cgttccggtt gaggagttgc tgaccgttca
      721 ggcgcaaaaa tggcaaattt tgcagaaacc gccagtattt tcattgggta accccattca
      781 tgattcggaa caaaaaatca ttgatgccgg aaaagaatta ctggaaaaag gtgcagatgt
      841 catcatgctg gattgtttgg gatttaacca gcgtcatcgc gatttactgc aaaaacagct
      901 cgatgttcct gttttgctct ctaacgtatt gattgcacgg ctggctgcgg aattactgat
      961 gtaattttgc gtgacagcca gcgcctctgg cccctatagt gaagtagatg ttcaactacc
     1021 agacagggcc agtttatgct tcaaagtaat gagtactttt ccggcaaagt gaaatcgatc
     1081 ggcttttcca gcagcagcac tggtcgcgcc agcgtgggtg ttatggttga aggtgaatac
     1141 accttcagca ccgctgagcc tgaagagatg acggtaatca gtggcgcgct gaatgtgtta
     1201 ctgcctgacg cgaccgactg gcaagtgtat gaagccggtt cggtgtttaa tgttcccggt
     1261 cacagcgagt ttcatctgca agttgccgaa cccacctctt atctgtgccg ctatctgtaa
     1321 ttcctcgcct tccccttgaa cgggagggca tttatctgaa atgtcctctc tttaacccat
     1381 aataatattt cctttgctgg gattttttcg atttccgata tattcataat tcatcaaggt
     1441 tgatataaat atcagcgaag atctccagat attgttgcgg aactggcgac gataaaagat
     1501 aaatcagatg atgaatggtg gcgtgcattg ctgcaaagtc gcaatgatca tttgcgccaa
     1561 acggcattgc gcaacgcaca taccccgatg ttgttaacga cattgacgga gtctcaggat
     1621 cggtcgctgg ctattaataa tccacagctg gctgccgatg tgaaaacggt gtggctaaaa
     1681 gaggagccat cattactttt atttgtcgat caacccgctc tttcgcagtt acgggattta
     1741 gtgaaaaccg gggcaacgcg gaaaattcgt agtgaagcgc gtcaccggct tgaggaaaaa
     1801 caatgacaca acgcccctgg agcaagttac agcgtaaaac gcataatatt gctgcgctaa
     1861 aaattattgc ccgccgtagc gaataattat atgcctggtg tagcttcgta cgccggataa
     1921 gacgcggcag gcgtcgcatc cggcattaaa ggtaaatcag caattaacgt tgtgcttcgc
     1981 cacctaatcc ttcaatcagg ttttgaatta acgctgccag ttcaccagtc atcaggatga
     2041 aatcggcatc aaaacgctgg gcgaaatctt cacggtcgat atcttcgttt tgatcgcgca
     2101 gctcgtcgca gaacttcaga cgcttgagcg aaccatcgtc gcacatcaca aactgaatgc
     2161 gctgctgcca gtcgagcgcc agtttagtca ccacttttcc ggcttcaatg tggttggtga
     2221 tctcttcgct ggtcagatct tgtttcttcg cgcggatcac gccgccatct tccagcagtg
     2281 atttcagctc ggcttcatca agcagctgga agccctgtgc tgtgctgccg gagcgtaccc
     2341 attcggtcag cgtcagttca atcggatttt ccatgctcaa tggtacaacc ggtaacgacc
     2401 ccaggctttt acgcagtaat gccagcgtat cttccgcttt tttggcactg gcgcagtcca
     2461 ccataatcaa accgttaacc gtgtcgatcc acatcattgt ctggctaaaa cggctgaaag
     2521 cacgcggcag cagagaatgc agcacttcgt ctttcagcga atctttttcg gttttcttca
     2581 gcttacgcgc ctgttccgct tccagtttgg cgattttcgc ttccagcgcc tgtttaatca
     2641 ccggagacgg gaggattttt tcttctttgc gcgcgcagat aacaatttga ccattggcaa
     2701 cgtgcgttaa cgcatcgctg tgcgatccca tcggaggaac ccagcccatc tttgccatgt
     2761 cctggctgcc gcatggggta aatgccatcg aggctagctg tttttccatc tcttctgcac
     2821 gcagcgaaat ctcgcggcta agacggtaaa ccattaaatt tttgaaccac agcatgataa
     2881 tttccacggc cttgtcgtta aatttagcgg gcatgataac gaattgtcgg cgggcttgca
     2941 ttgccaatcc ggttgtccgt ctctacgcta ttgatattga aaaaaataag gagagtaccg
     3001 tgcgtatagg tatcgattta ggcggcacca aaactgaagt gattgcactg ggcgatgcag
     3061 gggagcagtt gtaccgccat cgtctgccca cgccgcgtga tgattaccgg cagactattg
     3121 aaacgatcgc cacgttggtt gatatggcgg agcaggcgac agggctgcgc ggaacggtag
     3181 gtatgggcat tcctggctca atttcgcctt acaccggcgt ggtgaagaat gccaattcaa
     3241 cctggctcaa cggtcagcca ttcgataaag acttaagcgc gcggttgcag cgggaagtgc
     3301 ggctggcaaa tgacgccaac tgtctggcgg tttcagaagc agtagatggc gcggcagcgg
     3361 gagcgcagac ggtatttgcc gtgattatcg gcacgggatg cggcgcgggc gtggcattca
     3421 atgggcgggc gcatatcggc ggcaatggca cggcaggtga gtggggacac aatccgctac
     3481 cgtggatgga cgaagacgaa ctgcgttatc gcgaggaagt cccttgttat tgcggtaaac
     3541 aaggttgtat tgaaaccttt atttcgggca cgggattcgc gacggattat catcgtttga
     3601 gcggacatgc gctgaaaggc agtgaaatta tccgcctggt tgaagaaagc gatccggtag
     3661 cggaactggc attgcgtcgc tacgagctgc ggctggcaaa atcgctggca catgtcgtga
     3721 atattctcga tccggatgtg attgtcctgg ggggcgggat gagcaatgta gaccgtttat
     3781 atcaaacggt tccgcagttg attaaacaat ttgtcttcgg cggcgaatgt gaaacgccgg
     3841 tgcgtaaggc gaagcacggt gattccagcg gcgtacgcgg cgctgcatgg ttatggccac
     3901 aagagtaaaa aaaccaggca attggctcat catgcctgat gcgacgcttg ccgcgtctta
     3961 tcaggcctgc aaaatgtgcc agaaccgcgt agggcggata aggcgttcac gccgcatccg
     4021 gcaataagca acgtgctccg tgactgcaat ctaccccact ggtttcgcca gcaccggact
     4081 atccgccgct tgctggcgct tatagcgacc atacagcagc aacgacgaca tcgcagcaaa
     4141 cgaaagcagg gcggcaggta gcgccacgta attatatgcc agccccagcg tcagcatcat
     4201 accaccgcaa tatgcgccga cggcgctacc gaggttaaac gctatttgcc cacctgcggc
     4261 acctaataac tctccgcctt tggcattttg cagtaacaat atttgcagtg gtgctgaaag
     4321 ggcaaataat cccgcgcaac aaataaatgc aaaaataagc gacgttgttt tcatgccgcc
     4381 gaagaaaaag agcatcagca gtgccagcac aataataaag tcagttactg ccgcaatgcg
     4441 cagtggtgaa taacgtcctg aaatccggcc acttagcaca ttccccagca ccatccctag
     4501 cccaactaac atcataataa aagtcatcgc cgtttccgaa aaaccggaaa taaacatcat
     4561 gtatggcttt acgtagctga accaggcaaa tacacctgcg ttgccaaaca tggtggcggc
     4621 gaaaattaac cacggggccg ggctgcgcaa aaagtgaaat tgttcgcgta gcttcccttt
     4681 cgcctcgtcg cgaatatccg gcacccaaaa atagaccgat gccatcaccg caatattaaa
     4741 aacagcgatc aataaaaagg tgtaacgcca gctaaattcc tgacttaaat acgttcccag
     4801 cggaatgccc agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt
     4861 gactttgccg ggtttgataa tttttgataa cacgatcgcg ccgacgccaa aaaatgcgcc
     4921 atgcggaaag ccggatacca gtcgaccaat ggcgagcatc aggtaagacg aagagagcgt
     4981 gaacatggcg ttgccaatga cgcacaacgc caccagaaac aataagatat gtttgagtga
     5041 gtagcggctg gaaaagagtg cgatgattgg cgcaccgacc accaccccca gcgcataata
     5101 cgagatcata tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag
     5161 cacgcccata atgccaaatt cggccatccc caaaccaaac gtgcccagag ccaaagataa
     5221 aatgactttt ttcataccac catcacaacc accagaacgg ctaccaatca ccgtagccaa
     5281 taaagtgata gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta
     5341 ttcattatcc tgctgaatag ttatttcact gcaaacatac tttccagttt gctgtagccc
     5401 aggccgttga tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg
     5461 tggctaatca caccgatggt tttgccgctg gcgttcaggg catccagcgc atcaagggcg
     5521 gtatccagcg tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata
     5581 cgcgttttat ggctgaccag atctgaaagc gccagtgcca gcgccagact gacgaggaaa
     5641 ctttcgccgc cagaaagggt acgggtgtcg cgtaccgcat ctgcctgcca ggtatcaaca
     5701 acctcgactt ccagcgcctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg
     5761 gtaagttgct gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg
     5821 cggaatttat cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc
     5881 tgctgcgtca tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttgtctgca
     5941 tcctgcttca gctgctggcg aatctcgccc tgactggtgg tgttttcacg caacttttgc
     6001 tgagtttgcg ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca
     6061 ggtcggtgtt gctgatgctg tgccagcgtt tcagctgtct gagtgaccag cgtttgcgcc
     6121 tgacggcgct ggttttccag attctgtttc agctgttcca gctgcgttag cgtctgctca
     6181 tccattaacg ccgcaagaaa tgcctgctga tcgtcaaaaa tgctgtcctg tagcgcggtg
     6241 tcaaactgcg cctgggcttt ttgcagactt tgcgctgcca gagcctcctg ttgctgcaac
     6301 gtttgctgct ggctttgcaa cgcgaggcat tgttcgtgaa cttgccgcca gttttccagc
     6361 gctacagttt cttcgctgtg cgggagttca tcactttgcg gcaacgtttc cagaatcggc
     6421 gtcagttgct gcatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc
     6481 tgcgcttctt gctgacgcgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc
     6541 agtgcataac ccgccaatgc cgttaagagc tgctgctggc gttgttcaat ttgctgttga
     6601 taatggataa tttgctgatt atgcgcggca atctgccctt gtaattcatg ccgttggctg
     6661 agtaaccgca gctggcgttc gtgctcatct tgtgcatcca gccacggttg aatatcgtcc
     6721 tgtggctgca aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct
     6781 tgctcatctt gtcggaggct ttgcgcttcg ttttcatcac gctgaagttg ctttgttaag
     6841 gcgtccagtt gcccacgtag cgtcgcacct tcttcaccga gctttttaac ttcgttttcc
     6901 agcgccagta atcgagactg attaacgcca ggctccagcg cctgatacgc ctcgaccgcc
     6961 gggtggctgg tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgttga
     7021 gcttccagcg ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc
     7081 tgcgtctttt ctttataacg ctggcgcatt tcgttaagtg cggcgttacg ttgcgtctgt
     7141 tcttgcgtga cattctggat agcgacctgt aattgcgcca ggcgtttttg ttggggaaca
     7201 atctgtccat gcagcgcgac caggcgctga cgcagtgggc gttgctcagc atgttgcgcc
     7261 agggcgctag caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt
     7321 ttttgctctg catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt
     7381 tgttgggaga actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg
     7441 tgttcctgta accaggcatt cagactttgt tgctgctgct gtaattctgc tgactgcttc
     7501 gccgcgtggt ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact
     7561 tcttcaatct gctggcgagt atgcgccagc gccgcgctgt gttctgcgat gcgttcccag
     7621 tgcggacgaa gatttcgtgc cggttgtgcc agactgagcg ccgccagttg aggttgcgct
     7681 ttttcttcta cggctaacgc ctgttgcaag gcctgttgac ggcggctggc ttcttgctgc
     7741 aattcgtcca gacgcgttaa ccagtttagt gattgttgtt cttgctgctg cgcggtaagt
     7801 aactgttttt cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttct
     7861 ggcgtgagca acgcgacgcc gctggcctgc gcttgcagct tctccagctc tgtgcgggct
     7921 gatttgtgct gctcaaaaac catcgccgag atttgcccgt agatttcagt gccagttaac
     7981 tcctcgagca attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc
     8041 tgcgaaagca gcatcgaacg ggtgaagcgc ccgtaatcca gtccggttaa cgtcgccgtc
     8101 agttccagct tatctttcac tttgtcggcg agaattttgc catcggcgca gcgcgccagc
     8161 tctacgcgtg gcacctgcaa attaccgtcc ggctggttac gcgcccgatt ctggctccag
     8221 aacgcgcgat acgcttcacc tttcacttca aactccacct ccgccagaca ttcggcggta
     8281 tcgcgggtca tgagatcatt ttgcgattgt gaaacgttag aaaggcgcgg agtttcgtga
     8341 tacagcgcca gacaaatggc gtccagcagg gtggttttcc ccgcaccggt tgggccggta
     8401 atggcaaaca gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct
     8461 tttaatgagt tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg
     8521 cgagggtatg caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca
     8581 gttcttccag tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt
     8641 cacgctgttg gctggctaac actcgctcgc gctgttcacg actccggcgt accagcaata
     8701 cttcgacagg caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact
     8761 catcagtagt gatttcgata tccagccaga ccggtggctc ctgtgataca tcgcgccact
     8821 gttccagctg ttcagtaatc gacgccagat cgcctttcag caccgccatg ggttgcgtta
     8881 ccggtacgtt caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga
     8941 catatttact cttaccgcat tcatcaaaac tcagtggaat gggggagccg caatagcgaa
     9001 catgttccat gccgccaata atctgcgcgc ggtgaatatg cccaagcgcg atgtagtcgg
     9061 ctggcggaaa gttttgtgcc ggaaacgcgt ccagcgtgcc aatatagatg tcacgcacgg
     9121 catcactttt actggcaccc acagttgtta aatgtcccgt ggcgatgatg ggcagaggct
     9181 gagcgccgcg cagtttgcag gcatcggcat agtgttgttg ataataatca gtaatagccg
     9241 ccagcaaatg ctgctgtttt tcaataccgt taagccccgc ctggctggta ataatgtcac
     9301 gtgggcgtaa aaacggaatg gggcacagca ctgcccctgg cgtcccgtcg cgacgaggca
     9361 agatttgcgg cgcatgtccg gcgctggcga ccacggtcgt attgaggaac gccatgatat
     9421 cgcgcgattc attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac
     9481 agccagtttg ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactggggg
     9541 gcgagccggt atcgaaaaca tcaccggcaa caataatcgc atccacctga tgggtttgtg
     9601 ctgtctccag cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga
     9661 agttctggcc gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa
     9721 aaagcatggg cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga
     9781 aatcaataac ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc
     9841 ataatgacgt cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag
     9901 acgtattctg gtcgtagaag atgaagcacc aattcgcgaa atggtctgct tcgtgctcga
     9961 acaaaatggc tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa
    10021 tgaaccctgg ccggatttaa ttctccttga ctggatgtta cctggcggct ccggtatcca
    10081 gttcatcaaa cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac
    10141 cgccagaggg gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat
    10201 caccaagccg ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat
    10261 ttcgccaatg gcggtggaag aggtgatcga gatgcagggg ctaagcctcg acccgacatc
    10321 tcaccgggtg atggcgggcg aagagccact ggagatgggg ccgacagaat ttaaactgct
    10381 gcactttttt atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt
    10441 ctggggaacc aacgtgtatg tggaagatcg tacggtcgat gtccacattc gtcgcctgcg
    10501 taaagcactg gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata
    10561 tcgtttttca acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt
    10621 cttaactgga gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct
    10681 gctactttgc tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt
    10741 tttgctggca tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc
    10801 atggtggctg tgggtggatc gtagtatgac cccgccaccg ggacgtggta gctgggaacc
    10861 gctgctatac ggattacacc agatgcagtt gcgaaataaa aaacgccgcc gcgaactggg
    10921 caatctgatt aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac
    10981 cacggaagag ggcggtattt tctggtgcaa cggtctggcg caacagattc ttggcttgcg
    11041 ctggccggaa gataacgggc agaacatcct taacttgctg cgttacccgg agtttacgca
    11101 atatctgaaa acgcgtgatt tttctcgccc gcttaatctg gtgctcaaca ccgggcggca
    11161 tctggaaatt cgcgtcatgc cttataccca caaacagttg ctgatggtgg cgcgtgatgt
    11221 cacgcaaatg catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga
    11281 gttacgtacg ccattgactg tgttacaggg ttacctggag atgatggatg agcaaccgct
    11341 ggaaggcgcg gtacgtgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga
    11401 agggctggtg aagcaattgc tgacgctgtc gaaaatagaa gccgcaccga cgcatttgct
    11461 caatgaaaag gttgatgtgc caatgatgct gcgcgttgtt gagcgcgagg ctcagactct
    11521 gagtcagaaa aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa
    11581 cgaagatcag ctacgcagtg cgatttcgaa cctggtctat aacgccgtaa atcatacgcc
    11641 ggaaggcacg catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagcgt
    11701 tgaagataac ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta
    11761 tcgcgttgat aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt
    11821 gaaacatgcg gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg
    11881 aacacgtttc agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc
    11941 ctttgtcatc ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa
    12001 atacgggcgt tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct
    12061 ttttcacgga aatagtgttt tatactgttt ggtgatttct tatcgctata tacctctggt
    12121 ttttagatcc cttcttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt
    12181 catactgact gcattcacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga
    12241 gtatttcccg ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa
    12301 aatttaacgt tacaacacca catccacagg cagtatgatt tatgacccat caattaagat
    12361 cgcgcgatat catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca
    12421 ttattttccc tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg
    12481 gcttcctcat tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg
    12541 gcggcggtgt tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa
    12601 cggtgtgtta cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttctt
    12661 ttgaagtggg cattgcgccg ctgacgggtg attccgcact gccgctgttt atctacagcc
    12721 tggtttattt cgctatcgtt attctggttt cgctctatcc gggcaaactg ctggataccg
    12781 tgggcaactt ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgccgcga
    12841 ttgtctggcc ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcgtttt
    12901 ctaacggctt cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta
    12961 tcgttattgt taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt
    13021 ataccgtctg ggctggcctg atggcgggtg ttggtctgac tctgctgtac ctggcgctgt
    13081 tccgtctggg ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggt gcggcgatcc
    13141 tgcatgctta cgttcagcat acctttggcg gcggcggtag cttcctgctg gcggcgttaa
    13201 tcttcatcgc ctgcctggtc acggcggttg gcctgacctg tgcttgtgca gaattcttcg
    13261 cccagtacgt accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg
    13321 tggtgtctaa cctcggcttg agtcagctga ttcagatctc tgtaccggtg ctgaccgcca
    13381 tttatccgcc gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt
    13441 cgtcccgcgt gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga
    13501 tcaaagcatc tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg
    13561 aacaaggtct ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg
    13621 atcgcgcggc aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt
    13681 aaccacgggg ctgcgatgcc ccgtg
//
LOCUS       DARNVM010000130        13431 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.130, whole genome shotgun sequence.
ACCESSION   DARNVM010000130 DARNVM010000000
VERSION     DARNVM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.130"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..554)
                     /locus_tag="SI621_003470"
     CDS             complement(<1..554)
                     /locus_tag="SI621_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEI4066138.1"
                     /translation="MFPLNDLSLKAQSVQLNKITSNTESTIKQQDLVSDDAMINELSS
                     ELVSCLGNGRFTPISEDSKLLNMLSEFKLLHSEYFGWGDYSLWFQGFSIYDKTGFIMI
                     EKSQGAGNPPIRHTLEFISTNIAEFLDKLTKITDARLCKGFSDWANSVKEGASNDFKE
                     NVDIALVRLFKCVELHNSKLDLTDL"
     gene            complement(905..1219)
                     /gene="fliE"
                     /locus_tag="SI621_003471"
     CDS             complement(905..1219)
                     /gene="fliE"
                     /locus_tag="SI621_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEI4066139.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1434..3092
                     /gene="fliF"
                     /locus_tag="SI621_003472"
     CDS             1434..3092
                     /gene="fliF"
                     /locus_tag="SI621_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEI4066140.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3085..4080
                     /gene="fliG"
                     /locus_tag="SI621_003473"
     CDS             3085..4080
                     /gene="fliG"
                     /locus_tag="SI621_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEI4066141.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4073..4759
                     /gene="fliH"
                     /locus_tag="SI621_003474"
     CDS             4073..4759
                     /gene="fliH"
                     /locus_tag="SI621_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEI4066142.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4759..6132
                     /gene="fliI"
                     /locus_tag="SI621_003475"
     CDS             4759..6132
                     /gene="fliI"
                     /locus_tag="SI621_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEI4066143.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6151..6594
                     /gene="fliJ"
                     /locus_tag="SI621_003476"
     CDS             6151..6594
                     /gene="fliJ"
                     /locus_tag="SI621_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HEI4066144.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6591..7718
                     /gene="fliK"
                     /locus_tag="SI621_003477"
     CDS             6591..7718
                     /gene="fliK"
                     /locus_tag="SI621_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HEI4066145.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KAAPQLLVATDKPTTKGEPLVSDILSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TITLAAAADNNTAKDEKTDDLNEDVTASLSALFAMLPGFGNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTLAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIIPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFNGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7823..8287
                     /gene="fliL"
                     /locus_tag="SI621_003478"
     CDS             7823..8287
                     /gene="fliL"
                     /locus_tag="SI621_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HEI4066146.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8292..9296
                     /gene="fliM"
                     /locus_tag="SI621_003479"
     CDS             8292..9296
                     /gene="fliM"
                     /locus_tag="SI621_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEI4066147.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9293..9706
                     /gene="fliN"
                     /locus_tag="SI621_003480"
     CDS             9293..9706
                     /gene="fliN"
                     /locus_tag="SI621_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEI4066148.1"
                     /translation="MSDMNNPADNNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9709..10074
                     /gene="fliO"
                     /locus_tag="SI621_003481"
     CDS             9709..10074
                     /gene="fliO"
                     /locus_tag="SI621_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HEI4066149.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10074..10811
                     /gene="fliP"
                     /locus_tag="SI621_003482"
     CDS             10074..10811
                     /gene="fliP"
                     /locus_tag="SI621_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEI4066150.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10821..11090
                     /gene="fliQ"
                     /locus_tag="SI621_003483"
     CDS             10821..11090
                     /gene="fliQ"
                     /locus_tag="SI621_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEI4066151.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11099..11884
                     /gene="fliR"
                     /locus_tag="SI621_003484"
     CDS             11099..11884
                     /gene="fliR"
                     /locus_tag="SI621_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HEI4066152.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPTNDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12174..12797
                     /gene="rcsA"
                     /locus_tag="SI621_003485"
     CDS             12174..12797
                     /gene="rcsA"
                     /locus_tag="SI621_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HEI4066153.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12841..13029)
                     /gene="dsrB"
                     /locus_tag="SI621_003486"
     CDS             complement(12841..13029)
                     /gene="dsrB"
                     /locus_tag="SI621_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HEI4066154.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13192..13419
                     /gene="yodD"
                     /locus_tag="SI621_003487"
     CDS             13192..13419
                     /gene="yodD"
                     /locus_tag="SI621_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HEI4066155.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
BASE COUNT         3322 a         3446 c         3495 g         3168 t
ORIGIN      
        1 agatctgtta agtcaagttt actattgtgg agttcaacac atttaaacaa tctcaccaat
       61 gctatatcca cattttcttt gaagtcattc gatgcgcctt ctttgactga attagcccag
      121 tcactgaaac ctttgcaaag tctggcatct gtaattttcg ttaacttgtc tagaaactct
      181 gcaatattag tcgatataaa ctctaatgta tgccgaatgg gtggattacc agctccctga
      241 cttttttcta tcatgataaa acctgtctta tcataaatcg agaaaccttg aaaccagaga
      301 ctataatcac cccatccgaa atattctgag tgcaacaact taaattctga cagcatattc
      361 agtaatttgc tgtcttcact aattggtgta aacctaccat ttccaagaca actgactaac
      421 tcacttgata attcatttat cattgcatca tcagatacta aatcttgttg ttttatcgta
      481 gactcagtat tagatgtgat tttatttaat tgaaccgact gagcttttaa cgacagatca
      541 tttaatggaa acatttacac tcctgtttaa taaattatat tgcccaggga gcattctaaa
      601 acacaataaa attaccacta tcaaaaatcg ctcattttta taacgcagta tattaataaa
      661 aataagaacg aagattaaat tatccagagg ttactgttgc gtctgtggtc gtaacaacat
      721 ccgacgtggt gatgagtgtc aaatgagaca ctctgatggt caaagggttt attgcgttta
      781 cggatcgtgg atggcagaaa acgttcagga tcaggtatcc attttaaacc agaaatagag
      841 tgagtttgcc ccatccatgc cccacgcggt gagatcggat gtaattaaca acaggttaca
      901 caacctacac ctgcatgctc atcacttcct gatacgccgc caccagctta ttacgcacct
      961 gaatccccat ttgcattgaa actgaggctt tttgcatatc ggtcatcaca tcgtttaacg
     1021 ccacgccggg ttcaccgaga gtgaattttt ctgcctgcgt gcgggcggct gtttgtgtat
     1081 cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca
     1141 gtgattcctg cgcacgtgca ctcatcgccg tggtctgtaa ctggctgata accccttcaa
     1201 tcccctgtat cgctgacatt ttcatctccc ggataatttc tggtagcaaa gcctaccagt
     1261 aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg gcggatagaa
     1321 aaaaacgaaa gcacaaataa tggcagcgtc aatttttcga gtttgctgac ccgggagtga
     1381 gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc gcgatgaatg
     1441 cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa
     1501 ttccattgat tgttgccggt tccgcagcag tggcggtcat ggtcgcactg atcctgtggg
     1561 cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat ggtggcgcaa
     1621 ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc agcggcgcta
     1681 ttgaagttcc ggcagataaa gttcacgaac tgcgtctgcg cctggcacaa caaggtttgc
     1741 ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt
     1801 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa
     1861 ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
     1921 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
     1981 tcgatgaagg gcaaattagc gccattgtgc atctggtttc cagcgcggtt gctggtctgc
     2041 cgccggggaa cgtcacgctg gtggatcagg gtggacatct gttaacccag tccaatacca
     2101 gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccggattc
     2161 agcggcgtat tgaagcgatc ctgtcgccaa ttgttggtaa cggtaatatt cacgcccagg
     2221 ttacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
     2281 gtgatgaatc tcaggcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
     2341 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggctcct gcgaataacg
     2401 cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca
     2461 gcaatagtgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
     2521 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtccgtc gcggtcgtgg
     2581 tgaattacaa aaccttgcca gatggcaaac cgttgcctct cagcaacgaa cagatgaagc
     2641 aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca
     2701 atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagaactg ccgttctggc
     2761 aacagcaagc gtttatcgat cagttacttg ctgccggtcg ctggttgctg gtactgctgg
     2821 tggcgtggct gctgtggcgt aaagcggtac gcccgcagct aacacgtcgc gctgaggcga
     2881 tgaaagctgt acagcaacag gcacaggccc gcgaggaagt ggaagatgcg gtggaagtcc
     2941 gcctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgtctg ggggcagaag
     3001 tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca
     3061 ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc
     3121 ctactgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc
     3181 gaagtgcaaa ctctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta
     3241 accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact gaatatcaac
     3301 gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc
     3361 ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt
     3421 atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc
     3481 attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt
     3541 ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc tgccgcgctg
     3601 gcggagctga ccgaagttct gaatggcttg ctcgacggtc agaatctcaa gcgcagtaaa
     3661 atgggcggcg tgagaacagc agccgaaatt atcaacctga tgaaaactca gcaggaagaa
     3721 gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag
     3781 atgttcctgt tcgagaatct ggtggatgtc gacgatcgca gcattcagcg actgttgcag
     3841 gaagtggatt ccgaatcgct gttgatcgcg ctgaaagggg ccgagcagcc actgcgcgag
     3901 aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac
     3961 cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct gctgattgtg
     4021 cgccgccttg ccgaaactgg cgagatggta attggcagcg gcgaggatac ctatgtctga
     4081 taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg cagagtttgt
     4141 gcccatggtc gagccggaag aaaccatcat tgaagaggcc gaacccagcc ttgagcagca
     4201 actggcgcaa ctgcaaatgc aagcccatga acaaggttat caggcgggta ttgccgaagg
     4261 tcgccagcaa ggtcacgaac agggctatca ggaaggactg gcccaggggc tggagcaagg
     4321 tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag
     4381 cgaatttcaa actacccttg atgcacttga tagtgtgata gcgtcgcgcc tgatgcagat
     4441 ggcgctggag gcggcacgtc aggtgatcgg tcagacgcca acagtggata actcggcact
     4501 gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta aaccacagct
     4561 gcgcgtgcac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ccttaagttt
     4621 gcatggctgg cgcttgcggg gcgatcccac actccatcct ggcggctgta aagtctccgc
     4681 cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc
     4741 agcaccagga gtggtgtaat gaccacgcgt ctgactcgct ggctaaccac gctggataac
     4801 tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct
     4861 accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtgatt
     4921 gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt
     4981 caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgtt
     5041 tatgccaaaa acatttcggc tgaagggctg caaagcggca agcagttgcc gctcggtccg
     5101 gcgttattag gtcgcgttct ggacggcagc ggtaaaccgc tcgatggcct cccctccccc
     5161 gatacgacgg aaaccggtgc gctgattacc ccgccattta acccattgca acgtacaccg
     5221 attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcgggcgc
     5281 gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct gctggggatg
     5341 atggcccgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgagggcgc
     5401 gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg ctccgtggta
     5461 attgccgctc cagcggatgt ttctccgctc ctgcgaatgc agggtgccgc ctatgccacg
     5521 cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc
     5581 acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc ccccgccacc
     5641 aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat
     5701 ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga aggcgatgac
     5761 cagcaggacc ccattgccga ctccgcgcgg gccatcctcg acggtcacat tgtgctgtct
     5821 cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagtcgc
     5881 gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg
     5941 ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gtgcgtatgc caaaggcagc
     6001 gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta tttacaacaa
     6061 ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg
     6121 acagtgtcat aacccaggag ataacggcag atggcagaac atggtgcgct ggcgaccctg
     6181 aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc
     6241 ggatgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa tgaatatcgc
     6301 aataacctca atagcgatat gagtgccggg atgaccagca accgctggat caactatcag
     6361 cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact taatcagtgg
     6421 acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc
     6481 tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat
     6541 cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga atgattcgca
     6601 tagcgccctt gattaccgct gacgttgata acgccacatt gcctggcggc aaagccagcg
     6661 atgctgcaca agattttctc gcgttgttga gcgaagcatt aacaggcgag gcaacaaccg
     6721 acaaagcggc ccctcagttg ctggtggcaa cagataagcc cacgacaaaa ggcgagccgc
     6781 tggtcagcga tattctttca gacgcgcaac aagcagattt actgatccct gtggatgaaa
     6841 caccgcctgt catcaacgac gaacaatcca catcaacacc gttaaccacc gctcagacga
     6901 taacgttggc tgcggcggct gacaacaata cagcaaaaga cgaaaaaacg gatgatctga
     6961 atgaagacgt caccgccagc ctgagtgccc tttttgcgat gttgccgggt tttggcaata
     7021 cgcccaaagt gactgatgca ccgtcaaccg tgttaccgac agagaaacca acgcttttca
     7081 caaaactgac ttctgcgcaa ctcacaacag cacagcctga tgacgccccc ggcacactag
     7141 ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc atcagcacgc
     7201 cttcgccggt gaccgctgcc gccagcccgc taatcattcc acaccagaca cagccactgc
     7261 ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg caacaatcat
     7321 taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc
     7381 acccacagga tttaggtgaa gtgcaaattt ccctcaaagt ggatgataac caggcgcaga
     7441 tccagatgat ttcaccgcat cagcacgtac gcgccgccct ggaagcagcg ctgccggtac
     7501 tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatt agtggcgaaa
     7561 gctttaatgg tcagcagcag gccgcttccc agcaacagca aagccaacgc acagcaaacc
     7621 atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct ttacaagggc
     7681 gtgtaacagg taacagcggc gttgatattt tcgcctaacg tcagaggtag caccgtaatc
     7741 cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga taagcagtag
     7801 cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa gcaagcgatc
     7861 gctttggatc ccaattctgg tattcattac cctcgcggcc tgtgccagcg caggttacag
     7921 ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc
     7981 cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg atcgcgtgct
     8041 ttatatcggc ataaccctgc gactgaaaga tgaagcaacc cgctcgcggc tgagtgagta
     8101 tttgccggaa gtccgtagtc gcttgctgtt actgttttcg cgtcaggatg ctgccgtact
     8161 ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacac tttccacccc
     8221 gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg cttttattct
     8281 gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg ctgttgaatg
     8341 gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt gacattcgtc
     8401 cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca
     8461 ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg cgccgtagcc
     8521 cggatattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc cgcaacctgc
     8581 cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca gggctggtgg
     8641 tgttctcacc gagtctggtg tttatcgctg tggataacct gtttggcggc gatggacgct
     8701 tcccgaccaa agtggaaggc cgcgagttta cccataccga acagcgcgtc atcaaccgca
     8761 tgttgaaact ggcgcttgaa ggctatagcg acgcatggaa ggcgattaat ccgctggaag
     8821 ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc tcgccgaacg
     8881 acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc gaatttaata
     8941 tctgcctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac ccgccgctgg
     9001 aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag gtgcagcatt
     9061 cacagctgga actggtggcc aactttgccg atatctcgct acgcctgtcg cagattttaa
     9121 aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc gcccatgttg
     9181 acggcgtccc ggttctgacc agtcagtacg gcaccctcaa cggtcagtat gcgttacgga
     9241 tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc aaatgagtga
     9301 catgaataat ccggccgata acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt
     9361 gagcgagcaa aaatcaacca gcagcaaaag cgctgccgac gcggtgttcc agcaatttgg
     9421 cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata ttccggtcaa
     9481 gctgaccgtc gagctgggcc gcacacgaat gaccatcaaa gagttattgc gtctgacgca
     9541 agggtccgtc gtggcgctgg acggtctggc gggcgaaccg ctggatattc tgatcaacgg
     9601 ttatttaatt gctcagggcg aagttgtcgt agtcgctgat aaatatggcg tgcggatcac
     9661 cgatatcatt actccgtctg agcggatgcg tcgcctgagc cgttagtgat gaataaccac
     9721 gctactgtgc aatcttccgc gccggtatct gctgcgccac tgctgcaggt gagcggcgca
     9781 ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg gctgggattt
     9841 gcccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc actgggcgcg
     9901 cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca
     9961 gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga gataccgcag
    10021 accgattttc agtcggttat gaaaaacttg cttaagcgta gcgggagatc ctgatgcgtc
    10081 gtttgttgtc tgtcgcacct gtccttctct ggctggttac gcccctcgcc ttcgcgcaac
    10141 tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc
    10201 aaacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg atgatgacca
    10261 gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcgttggga acaccctccg
    10321 cgccacctaa ccaggtattg ctgggactgg cgctgttttt gacctttttt attatgtcgc
    10381 cggtgatcga taaaatttat gtcgatgcgt accagccatt cagcgaagag aaaatatcaa
    10441 tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc
    10501 gtgaggcgga tttagggttg tttgccagac tggcgaatac tggcccgttg cagggacctg
    10561 aagctgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat
    10621 ttcagatagg cttcacgatt ttcattcctt ttttgattat cgacctggtg attgccagcg
    10681 tgttgatggc attggggatg atgatggttc ccccagccac aattgctctg ccctttaaac
    10741 tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg gcgcagagct
    10801 tttacagcta gtgaggcaaa atgacacctg aatcggtcat gatgatgggg actgaagcga
    10861 tgaaagtcgc gctggcactg gctgccccac tgttgctggt ggcgctggtc acgggtctta
    10921 tcatcagtat tttgcaggcc gccacccaga ttaacgaaat gacgctgtcg tttattccga
    10981 aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc aatctgttgc
    11041 tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag gccgtacaat
    11101 gatgcaggta acaagcgatc aatggctttc ctggttaagc ctgtacttct ggccgttact
    11161 gcgcgtgctg gcgctcattt ctaccgcacc aattctgagc gaacgcagcg tgccgaaacg
    11221 ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat tacctaccaa
    11281 cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga tcctgatcgg
    11341 cattgcgctt ggttttacca tgcaatttgc ctttgccgct gtgcgaaccg ctggtgaaat
    11401 tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca gccatctcaa
    11461 tatgcccgtt ttagcgcgta tcatggatat gctggcgtta ctgctgttcc tgacatttaa
    11521 cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc caattggtgg
    11581 cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt tgattttcct
    11641 taacgggctg atgctggcat taccgctcat tactctgctg ctgaccctga atctggcatt
    11701 aggcttactt aatcgtatgg ccccgcaatt atccattttt gttattggat ttccattaac
    11761 tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcgc ctttttgcga
    11821 acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat tgccattaat
    11881 ataattccgt aacgtttatc atgttatcct aaggattatc cgaaaaataa tacctacgaa
    11941 catcttccag gatactcctg cggcgaaata tttgttttaa gctcactcac atatcgcaac
    12001 atttacttta ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt
    12061 ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg
    12121 atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa
    12181 cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta
    12241 gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt
    12301 gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc cacgatgctt
    12361 ctaacagtca gcgtatcaag ctcatcatta atcaacatcc caatacgtta tttatcgttt
    12421 ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattaa
    12481 tcagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa
    12541 aagagacaac gataacctcg tttttaaata tgccgacgtt atcattgagc cgaaccgaat
    12601 cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga
    12661 atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacgc
    12721 ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt
    12781 ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg ctgaattaga
    12841 ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat
    12901 gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac
    12961 tgccagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt
    13021 caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg
    13081 cagcgccagg cgcgcgcgag tgttgatttt ctcgacggtc tatacttaaa agatgccagg
    13141 cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc
    13201 gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg
    13261 gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac
    13321 gttagcgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg
    13381 gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa a
//
LOCUS       DARNVM010000131        13401 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.131, whole genome shotgun sequence.
ACCESSION   DARNVM010000131 DARNVM010000000
VERSION     DARNVM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.131"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..748)
                     /gene="yhaJ"
                     /locus_tag="SI621_003488"
     CDS             complement(<1..748)
                     /gene="yhaJ"
                     /locus_tag="SI621_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HEI4066156.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMV"
     gene            853..1554
                     /locus_tag="SI621_003489"
     CDS             853..1554
                     /locus_tag="SI621_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEI4066157.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGTAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGAMGSLQFRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1577..1741
                     /locus_tag="SI621_003490"
     CDS             1577..1741
                     /locus_tag="SI621_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066158.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1801..3111)
                     /locus_tag="SI621_003491"
     CDS             complement(1801..3111)
                     /locus_tag="SI621_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HEI4066159.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3139..4470)
                     /locus_tag="SI621_003492"
     CDS             complement(3139..4470)
                     /locus_tag="SI621_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEI4066160.1"
                     /translation="MEIVSNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4746..6110)
                     /gene="tdcG"
                     /locus_tag="SI621_003493"
     CDS             complement(4746..6110)
                     /gene="tdcG"
                     /locus_tag="SI621_003493"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEI4066161.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6182..6571)
                     /locus_tag="SI621_003494"
     CDS             complement(6182..6571)
                     /locus_tag="SI621_003494"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HEI4066162.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6585..8879)
                     /gene="tdcE"
                     /locus_tag="SI621_003495"
     CDS             complement(6585..8879)
                     /gene="tdcE"
                     /locus_tag="SI621_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HEI4066163.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8913..10121)
                     /gene="tdcD"
                     /locus_tag="SI621_003496"
     CDS             complement(8913..10121)
                     /gene="tdcD"
                     /locus_tag="SI621_003496"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HEI4066164.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVCNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNASAEFA"
     gene            complement(10147..11478)
                     /gene="tdcC"
                     /locus_tag="SI621_003497"
     CDS             complement(10147..11478)
                     /gene="tdcC"
                     /locus_tag="SI621_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HEI4066165.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11500..12489)
                     /gene="tdcB"
                     /locus_tag="SI621_003498"
     CDS             complement(11500..12489)
                     /gene="tdcB"
                     /locus_tag="SI621_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HEI4066166.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12588..>13401)
                     /gene="tdcA"
                     /locus_tag="SI621_003499"
     CDS             complement(12588..>13401)
                     /gene="tdcA"
                     /locus_tag="SI621_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HEI4066167.1"
                     /translation="NDIEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVN
                     EISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRD
                     GRLDFAIGTLSAEMKLQDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQT
                     NMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFI
                     TIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3460 a         3616 c         3170 g         3155 t
ORIGIN      
        1 cgaccatcgg atacggcatc gtcgccacgc caagccccgc cagtaacgcc tgacgtttat
       61 cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg
      121 ggcgctcacg agcggtatcc gctaccgcaa ttccacgata tttcacgcgc gtcacttcag
      181 ataacggttc tggctcctga tgaatcgggt gatcaggcgc ggcaacatag acgttcatta
      241 aggtatagag tttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga
      301 taacgatatc cgcccgcccc tgctccagcc gttcccatgc ccccgccagc acttcggtga
      361 tgattgccag ttgggtattc gcttttgccg ccagtttgtc aattaacggg aaaaaggcag
      421 gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag
      481 cttccgcatc ggtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca
      541 acatcctccc gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat
      601 ccagctcttc ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct
      661 catccgccgc cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt
      721 ccagcgttaa tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg
      781 ggcagaatat ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag
      841 aggtcaagaa ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga
      901 tggttgcagg cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc
      961 tatgcctccc tgcgcgtgct taaccaggaa gtgctggccc caggtaccgc ctttcagccg
     1021 cgaacctatc ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc
     1081 gacagcgaag gcaatcatgt tcaggccagc gctggtgagg tgttgctgct ctctacccag
     1141 ccgggtgtca gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag
     1201 ctttggctgg atgcctgccc gcagcgagag aatccactga ttcaaaagct ggcgcttaat
     1261 atggacaagc agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattccgc
     1321 cagcaagtgt ggctgcatca tattgtgctc gacaaaggcg aaagtgcgaa tttccagttg
     1381 catgggccac gcgcgtattt gcaatcgatt cacgggaaat ttcatgcact tacgcatcat
     1441 gaagagaaag cggcgctgac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt
     1501 acgctggttg ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt
     1561 taacagggag atgatgatga gtaaaaaatc ggccaaaaag cgtcagccag tgaagcccgt
     1621 ggtggcgaaa gaacctgctc gcaccgccaa taattttggc tatgaagaga tgttgagcga
     1681 gctggaagcc atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctactgcgta
     1741 aagacaggtg ccggatgcgg cgtaaacgcc ttatccggca cgatacccaa aacctggcga
     1801 ttatctggcc ttgctcgcca taatctcgat aatctgccga tccgtttgct gcatcgaatg
     1861 gcttgctaac gcacacaggt tggcaatcga ctgctcaaca tcatgcgcca caatcccttc
     1921 attgccggtc acggcggtat catccagcgc cattaacacc gctttccacg cagccgaagc
     1981 actggtcgaa accttcatcg cgcagctgtt cgacgctcca tcgcaaatca tgccgctgac
     2041 atcgccgatc atactgctga tcgccatcga aatggtttca taacgcccat ccaccagcca
     2101 tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg gcacaaagcg cagacaaacg
     2161 cggtaactgg ttatggatgt aaatcgcgct caaatgcgaa agcatcagcg cacgcgccag
     2221 ccgttcatca tccgctccga agtgttctgc taccaccacc acaggcattg ttgcggtgat
     2281 cccctggtta cccgagccgg agttactcat tgccggaagc gtagcgccgc ccatacgcgc
     2341 atcggatgcc gcgctggtac gaatcacaat ggatgaagag agatctttcg ccagcaaacc
     2401 gcgcgcgcac tgtttttcca gcgtcgcacc aatatgcagc ccccagttac cgctcaaacc
     2461 ttcctgtgat aacgcgcaat ttaacttcgc agaatcgaga ataaagcgga tcgccgcaaa
     2521 cgggacttca ttgacgaact tcaggatctc agccagcgtc gtcctggaaa gcaccgtcag
     2581 cggcgactct tgctcgccct ctgtcacaca cgcctgctga gtaaacacca caccattgtg
     2641 cgtctcaata tgcacaatgt tggtatgccc gccgacgatg gtgacacacg cccacttctc
     2701 accgttccag actttggcgc gtgagaagag gatttcatcg caaggttcct ggatcttaac
     2761 tgagactttc cccgccgcca gcagtgcttt ggcatcggca attgcctgcg cagttgcgtc
     2821 tttcagcact tccagcccgg cgttagcatt tccacctaac gccccaagcg ccgccgcaat
     2881 cggcagcccc accattcctg taccgggaac ggtgacaccc agaccgttct tcatcagatt
     2941 tggcgaaacc caggcttcta cacgttcaac cggaccttcc agttcagccg cagcaaccgc
     3001 cgccgccagc gccagtgaaa tcggttcagt acaccccagc gccggtttta cctcctcctg
     3061 aacggcgagg atgtaacgct gccataacgg atttaaagtc gaatcaaaca tattaaaaac
     3121 cttaaaattt caggtaaatc aagaaaatgc caggaacgga gaaacacaaa gcaacaaacc
     3181 agtgacgata atcaggtaca gagacatccc tttgtatttg tgcaatgccg gtactttgta
     3241 aaccagccac gccgggatca ggcaccctac catgccgaaa atcgggctac agatagaggt
     3301 gaagctcaac accggggcgt tcagtacgat ggcgctccag gccagcaaaa tggcgaaaat
     3361 catgatgccg cgctgaacga gattttcgtt aatcttctcg gcaggtatct tgcgacgcag
     3421 gatgttcatt acgatccctt gcgttgcttc gcgaaagcct aaatagacgc caaagaatgc
     3481 agtcattaca gcaaagatat tgagaatgac gctgaccact ttcacccacg ctgcgccgtc
     3541 accgctaata aactgtgcgg caatcgccag cgcggaaata ttctgctcgt aggcttttac
     3601 cgcttcgtca tgtcccatcg ccagcgtgaa cgacacggcg tagaaaaaga cggtgacaaa
     3661 caaaatgcca aacgcgatat tcattgcccg caatgcttta tgccgcgcta cttcaatcga
     3721 tttttcccgc gagcgataag aaatcaccat cgggcttaac gtctggataa acagaatcga
     3781 cgtcagggta aacggcagcg taataatggc gtttttcacc agcagcccca gcggcggtag
     3841 cgaaccgacg ttatacagat gccacattcc caccatcgac acgcccagcg ccgcgaccac
     3901 cagcagcttg gtcagcacca tccccgtcga aattttgaat aacaatttct cgccgcgtga
     3961 ggagatcgcc accagaatgc aaatcagtac cagaccataa aagggactgt ctgacagcaa
     4021 cccttccgtc acgccgaagg tatgcaggta ggaagcacta tcgttggtga tggcggtgga
     4081 atagacgaac atccaaatca ccagcattac gaaatagagt gcacctaaca ggatgcccca
     4141 gtttttacct aaataaccgc taatgacgct cgggtaatct ttacattctg gtgattctgc
     4201 cagcgtatta ataaacaacc gctgaaacag atacattgcc gggtaaccaa tcaccgatga
     4261 gagcaaaaat acccacaatc ccatcaaacc gacctgcacc gggagaaaaa caatccccgc
     4321 gccaatcgcc atcccgatac tcataatcac ccagccggtg tcagtactgt cgaatttgat
     4381 cgcttctcgc cactcgctct cactcattcc agcacgaccc gccggggtcg aagcgtctgc
     4441 aatgacgcct ttattcgata caatttccat aatttctcgc tcaatatttt gtagggctta
     4501 ttattttttt ccgagccgca tcaaggcgat atgcggtcta cgtttttgca ggcgagttat
     4561 tagaataaag aaatgatacg cgcgaggtca gagaaaatct tcacaatcaa gactttgaaa
     4621 taacggagat ggataagaat tttctactaa attaatcaca agagaatatt cattctctat
     4681 ttgcggcaga tcacaaaaaa aaaggtgcac atttgtgcac ccaaggatga aagctgacag
     4741 caatgtcagc cgcagaccac tttaatggcc agtcctccgc gtgatgtttc gcggtattta
     4801 tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag tgaaacacgc
     4861 ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta ctgctttcac ggcattaatg
     4921 gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca ggtcagccca
     4981 aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact gccgcccagg
     5041 agttcagtta accctgccgc cgccattgaa caggccacgc caatttcccc ctgacagccg
     5101 acttccgcgc cggagatgga ggcgttcatt ttatacagcg cgccaatagc ccccgcggcc
     5161 agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc gtaataagcc
     5221 agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac gcgcccgcca
     5281 gctgcgtttt cttcactaac cgccagcgcg tacatattga tccagtcgat gacattcatc
     5341 ggatcgttag agatgttatc gctggaaacc agctgacgac gcagcgctac ggcacgacgc
     5401 ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc aataccgtca
     5461 tgcatcactt gccagatacg ggcaaaaccg gcgtcaattt ccgctttgct acgcagcgcc
     5521 agctcgttgt gcatcatcag accagatata gacaggccgt tgtaatcaca cattttcagc
     5581 agttcacctg ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg cgacaggccg
     5641 aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt acttaatagc
     5701 gcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg
     5761 ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact
     5821 ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca
     5881 tcctgcggac tgtttcctgc cagtcccatg ataatggcga catccgtggc atggcctttg
     5941 cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag
     6001 ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc
     6061 gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc
     6121 tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg ctcacgaggc
     6181 tttacgcact acgtactgcg atggcttcaa tttccagctt cacatctttc ggcaaacgcg
     6241 cgacctgtac acagctccgg gtcggatagg tcgcctgatg ctcatcgaag aactgcttat
     6301 agacttcgtt gatggtggca aaatcattca gatcggtgat gaacacggtc atcttgatga
     6361 tatcgcccac gctcagcccg gcagcaacca cgatcgcttt gacgttttcg aggcttaaac
     6421 gcgcctgatc ttgcacatca gccgggatct caccggtttg tgggcaaacc ggaatttgcc
     6481 cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg attgcgcctg
     6541 gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag cgcctgggta
     6601 aaagtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa gcgcacggcg
     6661 tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat ggcatccagc
     6721 agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac atccgcttcg
     6781 tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg atcttctttg
     6841 cccagcgccg caggcacaat cgagaaggtg tacgagatcc cgtctttggc gtaggtgaac
     6901 ggcagtttcg ccaccgacgt caatgaggcc acggcacctt tgcgatcacg accatgcatc
     6961 gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt attaccggtt
     7021 ttctggccgt acaccacgtt agaagtgata gtcagaatcg actgggtagg gacggcgttg
     7081 cgataggttg gcagcgcttt aattttcttc ataaagcgtt caaccagatc gcaggcaatg
     7141 ctgtctacgc gctcgtcgtt gttaccgtac tgcggatatt cgccgtcgat ttcaaagtcc
     7201 accgccaggc cgttttcgtc acggattggt ttcacgcggg catatttgat ggcagacagg
     7261 gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata gacatcacga
     7321 tcgtgcagcg ccatcagcga agcttcgtag ctgtacttgt cgtgcatgta gtgaatgata
     7381 ttcagcgcac tgatgtactg caccgccagc cagtccatga agtgatcgag actgtccatc
     7441 actttgtcgt aatccagcac gtcgtccatc aacggtgctg ttttcggccc gacctgaatc
     7501 ttcagcttct cgtccacccc gccgttaatt gcgtacagca gtgttttcgc caggttagcg
     7561 cgtgcaccaa agaactgcat ttgcttacca atcaccattg ggctgacgca gcaggcaatc
     7621 gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt cgttttcata ctgcaaggaa
     7681 gaggtgacga tcgacacctg cgcggcatat tttttgaagg caatcggtaa ttcttccgac
     7741 caaagaatgg tcaggttagg ttccggtgca ggccccatag tgtgcagggt gtgcaaatag
     7801 cggaaggagt ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc gatcacttcc
     7861 gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt ccggtgtacg caagaagcgc
     7921 accatacgga tcttcataat gaagtggtcg atcagttcct gtgcctgctg ctcattgagt
     7981 acgccagctt taaagtcgcg ctcaatgtag atatcgagga acgatgccgt gcgacccagc
     8041 gacatcgcgc cgccgttctg tgatttcact gccgccaggt aagcgaagta gagccactgc
     8101 accgcttcct gcgcattctg cgccgggcga gagatatcaa agccatattt cgccgccatt
     8161 tcctgaatct gcaacagcgc atgacgatgc tctgccagct cctcacgcag acggatggtg
     8221 gcttccagat cctcgccttt ttccagacga gactggagat cggcaaattg cagttcgcgt
     8281 tcacgcacca gataactgat gccatacagc gctacgcggc gatagtcacc gataatgcgc
     8341 ccacgaccat agccatctgg taaaccggtc agcacgccag atttacggca gcgcagcata
     8401 tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc ggtaaacaga
     8461 tattcaaatt cactgtccat ttcacgacca taggcgtgga atgaactttt aatcatatta
     8521 atgccaccga acgggtgtaa cgcacgtttc aacggcgcat ccgtttgcag gccaacaatt
     8581 ttttccagcg gctggttaat atatcccgca tcatgagcgg taatggtggt ggcaatattg
     8641 gtatcgaaat caaccggcgc gtgggttgcg ttttcaatac gaatgccttc catcaccttt
     8701 tcccacaatt ccgtggtggc tggcgtcgct tcagcgagga aagattcatc gccttcatac
     8761 ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca gtccgtacct
     8821 ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc taccttcatg
     8881 aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcgga cgcgttcact
     8941 ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttggttgg gataacggcg
     9001 caaatgacac gggcattttc actggaaaca attcgctctc cacaggagtt agagcgatta
     9061 ttcatttctg tatcaatctc tacgcctaat acagccaaat gttccatgac cagacgacga
     9121 attaaacttg aattttctcc tattccgccg gtgaatataa ttccatccag gcgacgtaat
     9181 gaggctgcgt gtccggcaat atgacgggca attcggtgaa caaaggtttt aattgccagt
     9241 tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc cgaagaaaga
     9301 ccggaaatac ctaataatcc cgactcttta ttcactacgc gttccaggtc acccaggctc
     9361 tggttggttt ggctggcgac ccaggacatc gcaccaaagt cgacatcacc actgcgggta
     9421 cccatcatca agccttccag cggcgtcatt cccattgaag tgtcaacact ctgaccgttg
     9481 caaactgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc ggagtcatct
     9541 tccgccagat tcagcaaaga atgcgcgcgc tgggaaacat agcggtgcga cgtgccgtgg
     9601 aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc gtataaataa
     9661 gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgccacctg agttacgcca
     9721 ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg caggggtgcc
     9781 agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc ggactcggta
     9841 aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc atttaaattc
     9901 cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg gtgagccagc
     9961 ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat accgtcggca
    10021 atacctgaca ttaatacttc acagtcgctg gcgtcgagta cggaaaactt aatcgaagac
    10081 gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct catcctgagt
    10141 tacggattaa aacagtttgt atacgatgtt caggatggtc agcagaccaa tcacggtaac
    10201 aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac ggatggcata
    10261 catcggcaac aggcacagca gggatgcgat aatcggtgcg cccatggctt caatcaggtc
    10321 gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga agatcatgct
    10381 gagagtattc agtttaccca gcgacacttt ggttttgtca cctttataac cgaacttcag
    10441 aatcaggcca ttcaagcctt ccagtgtccc cagatagtga ccgaagaaag atttgaagat
    10501 agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg ttgttttggt
    10561 accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat tctgcgcttt
    10621 ggcttccgcc atgttggccg gagacagcgt aaacaggcag ctaaaggcaa agaacatcac
    10681 cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac gttcggtgaa
    10741 gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg aagagacgat
    10801 tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc agccagacag tgatcaggat
    10861 accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa ctgcagagtt
    10921 ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg gccataccag
    10981 gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag ccatcagcag
    11041 caacaggaac agcgccacaa agccgcgatt cagcggtgca aagccgagct ggttttccca
    11101 gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg ggcaaatcgc
    11161 gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct cttccaccgt
    11221 ttccgtaatg ttgccggaag ggttagagcc agaaagacac agacgcgcca gcgcccggtg
    11281 gcaataaaat gcgatggggt atgctaatac caacatcaga agaatcggga tcagtccgcc
    11341 aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac caaacaagcc
    11401 taacgtccat gtggtatctg atttacgcca tgacgattgt tttgtctggc tggatacaat
    11461 gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa cgaaaccggt
    11521 gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga cggtttttct
    11581 gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca gagcgcctgc
    11641 gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc tgatttcgtc
    11701 ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag ttaaattacc
    11761 cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg tggttatttc
    11821 tccggagtgg aaagaagccg ccatgccgtg aacgttttct gactgtacgc caataacacg
    11881 aatggtcggg ttaatagatt ttattgccac cgcaatacca gcaattaaac cgccaccacc
    11941 aattggcaca atcacattat cgacatcata gagatcttcc ataatttcca ggccaatcgt
    12001 tccctggcca gcaatcactt tcggatcatc ataaggtggg ataaaaatac ggccttccat
    12061 ttcgacaatt tcgctcactt tagcgatagt gtcgttgaag ttatcaccat gcagaacgac
    12121 ttctgcagag tagtcgcacg ttgccgctac tttggatttc ggcgcgcctt ttggcatcac
    12181 cactttaccg tcgataccca gcatcgcgca ggagagggaa accccttgcg catggttgcc
    12241 cgcagagcag gccactacac ctttacgttt ttccgcatcg gtcagtgaac ttaatttatt
    12301 aaatgcgcca cgaattttaa atgaaccggt acgctgcatg ttttcgaatt tgaggaatat
    12361 ttcacctttg caacgttcac taaaatagtt ggagcgaggc atgcctgttt tataaattcg
    12421 cccagccagt cgttgtttcg cttcaatgat gtcatcaata gcaaccggga gatcgtatgt
    12481 aatatgcatt ataaaacctc ttagcctgaa ttaaatatgt aggtaaccgt aaccgacacc
    12541 tgcaaaacag acaggtggat tatttatgtt taaataaaaa caaataacta accaacttca
    12601 attaattgcc ttcgtctaca cccattataa gatgaatact ctttggctaa ttccaccaaa
    12661 accgatgctg cttttttaat acgataattt ttcgaccata ccgcggcata ttgtgccaca
    12721 ggtaatgttt cttcaaccgg aatagtaata aattgatttg aaccaaaagg tgatgtcata
    12781 tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa gattataaat tgtcacgact
    12841 gagtcggttt taacgatgtt ttcaatactg atgccatttc tttgtaacgt agtaagtagc
    12901 tcgctgtagt accccatatt ggtttgtggc aacacccact gttcgttctt caacgactcc
    12961 agcgtggtgg tgccggtgca tgttcgggac ttactggcta ccagcacaaa ctcggactcg
    13021 aacagcggct caacatgtaa atcctgaagc ttcatttctg cgcttaacgt accaatcgca
    13081 aaatccagtc gaccgtcgcg gattgccggt aagaacgaag acagttgcgc ttcatacata
    13141 gaaacctgcg ctttcgggaa cacctctttg aacttgttga tcatcccgga cataaaagta
    13201 aaaccaatca atgaaggaaa accaaatgag acttccacca ccgcctcaga agacataccg
    13261 cttatctcat taaccatatt tttcatttca cgggtaatgg attcagaacg ggagagtaac
    13321 aattgaccgg caggtgttag tgttacaccg gtatttttcc gcaccactaa ttccacgcca
    13381 aaataatctt caatatcgtt a
//
LOCUS       DARNVM010000132        13215 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.132, whole genome shotgun sequence.
ACCESSION   DARNVM010000132 DARNVM010000000
VERSION     DARNVM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.132"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..763
                     /locus_tag="SI621_003500"
     CDS             <1..763
                     /locus_tag="SI621_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HEI4066168.1"
                     /translation="KVDNELTRRYEYARMDTENIYLLPLARGNNHNYDGKSVVEIRKL
                     NIGKQSWPFNYVTETCSEFNGITTTGRMLYRNLKITSALDEIYGGICKKAHAATELAE
                     GLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTNEQP
                     RIDIYIDQIMTVTGVANSSGFALAALLNANIGMGNDPIIGIEAYPGTAEIHAKMGYKV
                     ISGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(904..1677)
                     /gene="uxuR"
                     /locus_tag="SI621_003501"
     CDS             complement(904..1677)
                     /gene="uxuR"
                     /locus_tag="SI621_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HEI4066169.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1892..3352)
                     /locus_tag="SI621_003502"
     CDS             complement(1892..3352)
                     /locus_tag="SI621_003502"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HEI4066170.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3433..4617)
                     /gene="uxuA"
                     /locus_tag="SI621_003503"
     CDS             complement(3433..4617)
                     /gene="uxuA"
                     /locus_tag="SI621_003503"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEI4066171.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4957..6300
                     /gene="gntP"
                     /locus_tag="SI621_003504"
     CDS             4957..6300
                     /gene="gntP"
                     /locus_tag="SI621_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HEI4066172.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPVWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6473..7375)
                     /gene="fimH"
                     /locus_tag="SI621_003505"
     CDS             complement(6473..7375)
                     /gene="fimH"
                     /locus_tag="SI621_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HEI4066173.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGFYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7395..7898)
                     /gene="fimG"
                     /locus_tag="SI621_003506"
     CDS             complement(7395..7898)
                     /gene="fimG"
                     /locus_tag="SI621_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HEI4066174.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7911..8441)
                     /gene="fimF"
                     /locus_tag="SI621_003507"
     CDS             complement(7911..8441)
                     /gene="fimF"
                     /locus_tag="SI621_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HEI4066175.1"
                     /translation="MRNKPFYLLCAFLWLSVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8451..11087)
                     /gene="fimD"
                     /locus_tag="SI621_003508"
     CDS             complement(8451..11087)
                     /gene="fimD"
                     /locus_tag="SI621_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HEI4066176.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGSNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11154..11879)
                     /gene="fimC"
                     /locus_tag="SI621_003509"
     CDS             complement(11154..11879)
                     /gene="fimC"
                     /locus_tag="SI621_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HEI4066177.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11916..12455)
                     /locus_tag="SI621_003510"
     CDS             complement(11916..12455)
                     /locus_tag="SI621_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4066178.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12519..13067)
                     /gene="fimA"
                     /locus_tag="SI621_003511"
     CDS             complement(12519..13067)
                     /gene="fimA"
                     /locus_tag="SI621_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HEI4066179.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3198 a         3428 c         3216 g         3373 t
ORIGIN      
        1 aaaagtcgac aatgagttaa cacgtcgata tgaatatgca cgcatggata cggaaaatat
       61 ctatcttctg cctcttgctc gtggcaacaa ccataattac gatggaaaaa gcgttgtcga
      121 aattagaaaa cttaatattg gcaaacagtc ctggccattt aattatgtaa cagaaacatg
      181 cagcgaattc aatggcatta caacaacagg gcgtatgctc tatcgaaact tgaaaatcac
      241 ctcagcttta gatgaaatat atggtggcat atgtaaaaag gcgcatgctg ccacagagtt
      301 agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg ttgaggacta
      361 tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg ccggaaccac
      421 gataggatat atttcaaccc ttccggcttc tcaggcaaaa agatggacca atgagcagcc
      481 aagaattgat atctatattg atcagataat gacggtaacc ggggtagcaa attcttccgg
      541 attcgccctt gccgcattac ttaatgctaa tattggaatg ggaaatgacc caataattgg
      601 tatagaagcc tatccaggta ccgcggagat ccatgcaaaa atgggatata aagttatctc
      661 cggtgatgaa gatgcgccac tcaaacgcat gactctgcaa ccgtcatctc ttccagaact
      721 gtttgaatta aaaaatggtg aatggaatta tattggtaaa tgactactga gaagaatgag
      781 tactaacatt caggttatac agatatttaa acaataccct gcaatttaca aagtaatact
      841 cattgcgact tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat
      901 aagtcaggcg tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat
      961 atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat
     1021 tgccagcttc gccgcacggg catctttttt gattaacgcg gcgaggatct gtttgtgatc
     1081 gcccaaccac tctttgcgat acaggctgtc atcgagatgg ctgtgcaatt ggatccacat
     1141 tggattgttt tcccgccact gccaggactg acggaataac tccaccagca tgctgttatg
     1201 cgttgcttcg gcaatggcga gatgaaactg catgtcacca ctttcgctgc tgcccggcgc
     1261 gctggaagca agctcacgct cttccagttg caatgcctga cgcattttga cgatatcttc
     1321 gcgggtagcc tgcagggcgg caaattccgc gatattactt tccagtaact gacgcgcctg
     1381 taacagctca aaaggaccgg catcattgca gacgttggca tccggactgt ctgtgttgtg
     1441 gctgcctgag ctatcaagaa cataaatacc ggcaccccgg cgcacttcca ccagcccttt
     1501 gatctccagc atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc
     1561 aatttcacgc tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc
     1621 gcggatcatc gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt
     1681 gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgtcttatc
     1741 cggcctacaa cgggcaattt tacggcaccc gttaatctca aactatacca ttagcactgg
     1801 aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tgcgtttgat
     1861 tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac
     1921 cgcgttcgca cagctgctga tatgccgccg tcaccgcgcc aacaaagtcg gcattctgcg
     1981 gcagatcatc ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct
     2041 gatactgcgc gttaatcttc tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat
     2101 taccctgctc atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt
     2161 gacgccagct gccgccgttt tgcaggtgca gacgcaccgg atccaacaga cgctgcggta
     2221 acttctggct gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga
     2281 aacgctcgat cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg
     2341 ttggcgcttg ttcctgcatc atcagggcaa aggccgcttt gcgataatcc gggttagtca
     2401 tggtgtcggc aatggtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc
     2461 tgccgttcag catacgcagt ttcatcattt cgaacggcac aacgtctgca acgaactgtg
     2521 cgcccacttt atcccagtcc ggacgaccat taacgaagtt atcttcaatc acccactgac
     2581 ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct
     2641 gtaaggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga
     2701 cgttctcttc aatccatgcc gccagctgcg gatcacgtgc ctgagccagc cccagaaccg
     2761 cgaccttcgc cacatgaccg ttttcacgca cgttgtcgca ggacatcacc gtaaacgctt
     2821 tcagcccttt ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg
     2881 gcgcagtcgg gttttccaga tcgtgcttga tcagcgggtt attgagatcc aactgaccgc
     2941 ttgccgcatc ggcacagtag cctttttccg tcaccgtcag ggagacaatc gccgtctgag
     3001 gacgcgccat cgcgttgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt
     3061 tcatcgaacc gataattttc agctcggtgc tttctgcgcc tttttccgct acggtgtaca
     3121 gcagttgctg ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa
     3181 cttcgcagat gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct
     3241 ggtgcgcgcg gtgaaacgca ccgcaaccga gatgcacaat gcgtgattcc agacgagaat
     3301 gatcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact
     3361 ccagaatccg tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca
     3421 tgccggtgga tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac
     3481 ttcggccagg cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag
     3541 gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc
     3601 cgctttacga cggtgttctt cttcaacaat cgctttcacc acttcataca tatcaacgtc
     3661 accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg
     3721 cagatgggtg aagtaaatgc gcggaccaaa ctgcttgatc atatcaacca gatcgttgtc
     3781 agcacgcacg ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt
     3841 atcaaccatc cactgcatat cttcaatggt agaaacaatg cgcggcaggc cgaggattgg
     3901 gcgcggcgga tcgtccgggt gaacagccat acggacgcca acttcttcgg caaccggaat
     3961 aatggctttc aggaagacag caaagttttc gcgcagtttg gctttatcga tatctttgta
     4021 cagctccagg tgcttacgga actggtcgag ggtataccct tcttccgcac ctggcagacc
     4081 agcaatgatg ttacgggtca gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgaac
     4141 agctgcctga gcaatttctt cttcggtgta atccgcttcc gcgcctggac gcttcaggat
     4201 atgcatttca aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg
     4261 cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac
     4321 ggtgcgaatg ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg
     4381 ctcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggcacgcttt ctacgacaga
     4441 ccacaccagg cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca
     4501 tacttcgcca ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg
     4561 gacatcagct aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat
     4621 ttttcctctt tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta
     4681 ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata
     4741 agttctacct tttggtaaga catgtcaacc tgaattctaa aattggttaa ccacatcaca
     4801 agaatttcac tctcctggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg
     4861 atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca
     4921 aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg
     4981 gtggtattcg gcattggtct gatgctggta ctgaatttga agttcaaaat caattcaatg
     5041 gtggctttgt tggtggcagc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg
     5101 ctgctgcaca ccatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg
     5161 gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagca
     5221 catacgctgc tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc
     5281 cttattttcg gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt
     5341 attgttattg ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca
     5401 gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc gctggtgaat
     5461 gcttatggcg cagatatggg gatggtttat atctatggcg tactggtgac gatcccaagt
     5521 gtcatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca
     5581 tcattcctga aagcagatca accggttgat atgaataatc tgccctcttt cggcgtttcg
     5641 attctggtgc cgctgatccc agcgatcatt atgatctcca ccaccatcgc caatatctgg
     5701 ctggtaaaag atacccctgt ctgggaagtg gttaacttta tcggttcctc gccgattgca
     5761 atgtttattg cgatggtggt tgcattcgta ctctttggca ccgcgcgtgg tcatgacatg
     5821 cagtgggtga tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc
     5881 atcggtgcgg gtggcgtgct gaagcagacc atcatcgaca ccggcattgg cgacaccatc
     5941 ggcatgttga tgtcccacgg caatatctcg ccctacatca tggcatggct gatcaccgta
     6001 ctgattcgtc tggcgacggg tcagggtgtc gtttcggcga tgaccgctgc cgggattatc
     6061 agtgcagcaa tccttgatcc agcaactggt cagctggttg gcgtgaatcc ggcgctgctg
     6121 gtactggcga cggctgcggg ttccaacacc ctcacccaca ttaacgatgc atctttctgg
     6181 ttgttcaaag gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg
     6241 gagctggtca actccgtggt tgggctgatt attgtgctga ttattagcat ggtagcgtaa
     6301 aagaacacca gagcctgcca ttggcaggct ctgttcggat tgtcggtaaa gtgcgctttg
     6361 tttatgccag atgcgacgct gacgcgtctt atctggccta caaagggcta acgtgcaggt
     6421 tttagcttca ggtaatattg cgtacctgca ttagcaatgc gctgtaattt ttttattgat
     6481 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccggtac
     6541 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6601 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6661 ctggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg gttgtgccgg
     6721 agaggtaaaa ccccaggttt tggcttttcg cacaataaac ggtaagagga atcggcaccg
     6781 aaccgggata gtccggcaga gtaacggtga catcacgagc agaaacatca cagccgccag
     6841 taggcaccac cacatcatta ttggcgtaaa tattccacac aaactggaaa tcatcgctgt
     6901 tatagttgtt ggtctgtcgc aaaattagca cggcaattaa tgagccagct ttaatcgcca
     6961 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7021 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7081 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7141 gtgtgacata gtctgtaatg gtttccgggt aatcgttatg gcaaaagatt tgcgtcgaaa
     7201 gatctacgac caggttttgc cccacattca cggcgggcgc aaggtttaca taaacattgg
     7261 cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg aatgaccagg
     7321 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7381 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7441 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7501 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7561 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7621 tcggctgccc cgctgaagct ggcggtgacc ctcgacgttc ccaccggaca attagtcaac
     7681 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acatcagact gaaagaataa
     7741 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7801 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     7861 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     7921 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     7981 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8041 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8101 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8161 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8221 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8281 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8341 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgtgact taccgacaac
     8401 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8461 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8521 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8581 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8641 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8701 atcccaacgc gcgctttaaa ctctgctcgc actatcgccc cacgagtggg aacaacgtta
     8761 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     8821 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     8881 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     8941 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9001 ccataataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9061 ccaccgcgat aattcagcgt ggcgtagcct gtgcttccgc tattaccatc gcctcccccg
     9121 gcatagccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9181 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtagct ggcactggca
     9241 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9301 agcgctaaca tctgatcccg tcctttttgc caggcgtttt tcgtcaggct atagctgagc
     9361 gtccagttga tatcttcgaa tgcagtattt aaaccagcct ggaattgctc atcgacatta
     9421 ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatgt gcgcccgagt
     9481 tgctgagtaa cggtgagctg taatttcccg cgtttgttat aagcgaggtt gtaatagtcg
     9541 gtgaatttcg gcttaacctg aataactcca tcctgtgttt cgatgttgta gccattcatt
     9601 cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg gtaacccact
     9661 aactgaatat tcgtgcctga ctcattgagc gatttgttat agagaaaacg caccgattgt
     9721 ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc cacagacaga
     9781 gcgcccagtg cccccatgtt tttcccgata ccgaaattaa aagcacgata acgatccgcc
     9841 agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa tgtactctgg
     9901 aaaaagcgag gtttctcctg ctgcgcattt ccactacggt attctcctgc cgtaatggaa
     9961 taacgtgtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac ggtaaaaatc
    10021 tgcgtgctac cgtcagcctc tttaatcgtt acctgcaagt caccactatt acctgcggca
    10081 tagatatcgt tgatggtaaa aggccccggc ggcaccgtac tattataaat gtcataccca
    10141 ttttgtttaa tagtgacctg tgcagtacca cgcgcaatac cgtggatcac cggggcaaat
    10201 cctctttggc tatcgggtaa catattgtca tctgaggcca attgtgcgcc gcgaaagtta
    10261 ataccatcga aaatatcgcc ctgagtataa ccatcaccca gcgtcagccg ggaacgtaac
    10321 ggaattatgt ctcgctcaag ccaggtattg atatgctgcc atttattttt gctacctgat
    10381 gaactgtcgc tactgttata actccaggtg gtattgtcgc gtaaacgcca cgcaccaata
    10441 tttagcccac tctgtaggtt taaatatgca taatggctgt tactaccaat ccgattctgt
    10501 acactatttc cgctgaaatt ataattgagc aagccggcat taataccggg atcccacaac
    10561 tcaggaggaa tatatccacg cgcgcgatta ctcataaatg cctgagggat cgtcaggttg
    10621 agtcgctgct gaccaacatc cagatgcgca gtagcgtcat ggatcattga ggttaatggc
    10681 acgcaggcat catccgccag cagattcata ccggagacag aagccgtatt cagccccata
    10741 ctggcaagtt gcgcgcgtgt caggcaggga acaatccctt gttcactgtc gcccgtgtta
    10801 aatgtgacat cacgcgttgc catataacca ttattcaaat agatatcgac gcgatacgtc
    10861 cctggcggta attcttgccc attttcaaaa cgcgataaat cggccacagc ctggggatca
    10921 tccgctaaaa agcgcggatt aaaatagagt tcggcagatg acaaaggtgc ctgtgcggca
    10981 aaagcacagg cgacaaagag ccggacaaaa aaaccagcca aacgatgctt acgaatatgc
    11041 aagcattgtg tgtttcgctg gtaaagtctt aaattcagat atgacattat tcctccggtt
    11101 taaaatcacc cggcagtcaa ttctttttat tcaggcgaaa aattccccct gcgttattcc
    11161 attacgcccg tcattttggg ggtaagtgcg ccataatcat ttattgttcg gtaagtaata
    11221 ttgcttcctg catcagaagg caatttaacc gtgctttcgc ccattggagg caccaatgca
    11281 ttttcaagaa cccgggttcc ggcattcaac tctgttaccg tcaggtaata gggtgtcggg
    11341 ttaatcagcg tcagagaatt cgcgctacga cgaaatctta atttttctgc ggcctgatcg
    11401 ggtggcaacg ctaatttagc cgggcgatag tacagtttaa tgcggctgat aattgcgagc
    11461 tgtagcgtat tctcagtcaa ttttgattta tccattgacg gaatcgcttt aacgttcatc
    11521 cagaataaac tttcccgatc ctgtggcaat tggttatttg ttgcatcaag aatacgtaag
    11581 gtattctctt tttttccctt catcgcaaac agaggaggcg tcacgataaa acgaccatcc
    11641 tttacaccat cggcattttc cacccatgat tgaattaaat aggtgctatt ttcatcatta
    11701 tttgtcacgg caagttgcac ttgtttttgc cccgccggat aaattacgcg ggtcgcacct
    11761 aaggccactc ccgcttcagc gcgtccggca accatcattg ccatgaacat caggatacct
    11821 gccagcaagc agaatgttat ttcctgcgat ttccttacat tgacgttttt attactcact
    11881 gtcctgttcc tgttatcaca ttatctgctg aacaattact gataggttaa agagaaccag
    11941 gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg tagcacgata tttggcgatg
    12001 aaatgtagcg aagtagagcc tgagtaaagc cgtttccagt ttgctggagg acgattaatc
    12061 ggtacgaggt ttccttcatc atcaaataac gctacgccaa tatttgtggc tatccctggc
    12121 ccctctccca cggaaagcac atccggattt ttaccatccg cgacaccgtg aaacgccaca
    12181 cccacacgtt cactcaccac cgtgctacat tcccgtaaat gaataacaaa aggcaccggt
    12241 gcgctatctt cccctaccgc atgaaaccgg ttactgctga tttgccccat attgaccgtc
    12301 atttgtttat caccagcttc aactcggcaa gtttccgcaa taatgacgcc ctgaaattgc
    12361 atatttccgc cgggcagcgt ggtattccat ttatttccgg ccagagcaaa cattggcagt
    12421 aacgacgcta atagaaataa tctcttcctt ttcatcgtta tttttatcgc acaagggtgg
    12481 gcatccctgc ccgtaatgac gtccctgaac ctggtaggtt attgatactg aaccttgaag
    12541 gtcgcatccg cattagctgc gccaggggtt gcggcaccgg ttgcaaaata acgcgcctgg
    12601 aacggaatgg tgttggttcc gttattcagg gttgtttctg aactaaatgt cgcaccgtcc
    12661 agcgccagcg cagcacccgt tctgtccagg atctgcacac caacgtttgt tgcgctaccc
    12721 gcagctgaac tctgcagagc cagtacatca gtatgcgtag catcaattgc cgtacctaag
    12781 aaggcaacag cggcttttgt ggcaacagtg gtatcgcaat cattcagctg aatgttaaaa
    12841 ccaacggcag agctggttgc tccagcctgc ttcaggctag cggtacgaac ctggcccaac
    12901 tgaacggttt gatcaacaga gcctgcatca accgcgcaag cggcgttaac aacttcccct
    12961 ttaaagtgaa cggtcccacc atttaccgtc gtagtatcgg ccagagccgc tgcggaactg
    13021 agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt
    13081 aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
    13141 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccttttga
    13201 ctcatagagg aaagc
//
LOCUS       DARNVM010000133        13205 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.133, whole genome shotgun sequence.
ACCESSION   DARNVM010000133 DARNVM010000000
VERSION     DARNVM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.133"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1079)
                     /locus_tag="SI621_003512"
     CDS             complement(<1..1079)
                     /locus_tag="SI621_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066180.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPAVVLATRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARKG
                     LVQLSSATNSTSETLAATPKAVKAAYDLANGKYTAKNATTTQKGLVQLSSATNSTSET
                     LAATPKAVKAAYDLANGRQPANAILTALAGLATAADKLPYFTGVDRAALTALTSVGRA
                     ILSKPSTQGVLDYLGLGEGSALPVGV"
     gene            complement(1076..1687)
                     /locus_tag="SI621_003513"
     CDS             complement(1076..1687)
                     /locus_tag="SI621_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HEI4066181.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDDKWPEATKRALIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFQASAIHLIDNLRVNA"
     gene            complement(1680..2588)
                     /locus_tag="SI621_003514"
     CDS             complement(1680..2588)
                     /locus_tag="SI621_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HEI4066182.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLMLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(2593..2940)
                     /locus_tag="SI621_003515"
     CDS             complement(2593..2940)
                     /locus_tag="SI621_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEI4066183.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2937..3572)
                     /locus_tag="SI621_003516"
     CDS             complement(2937..3572)
                     /locus_tag="SI621_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEI4066184.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3639..4091)
                     /locus_tag="SI621_003517"
     CDS             complement(3639..4091)
                     /locus_tag="SI621_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEI4066185.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(4084..4551)
                     /locus_tag="SI621_003518"
     CDS             complement(4084..4551)
                     /locus_tag="SI621_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066186.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4514..4672)
                     /locus_tag="SI621_003519"
     CDS             complement(4514..4672)
                     /locus_tag="SI621_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HEI4066187.1"
                     /translation="MPGSDPETNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            complement(4659..5084)
                     /locus_tag="SI621_003520"
     CDS             complement(4659..5084)
                     /locus_tag="SI621_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HEI4066188.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRVNNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGAGLPDAVRRLHQRPACTDASDCPQRLPESEPLPDAGQ"
     gene            complement(5072..5488)
                     /locus_tag="SI621_003521"
     CDS             complement(5072..5488)
                     /locus_tag="SI621_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HEI4066189.1"
                     /translation="MKKLSLSRMLNVSLALMLALSLIYPQSVAVNFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLDVQIVTALFLQPVTWCRRAAGLVVTFISLVA
                     AGWIFTALIYLVASLFFRLMQTACRQRLEGRELCQS"
     gene            complement(5503..6000)
                     /locus_tag="SI621_003522"
     CDS             complement(5503..6000)
                     /locus_tag="SI621_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HEI4066190.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(6000..6281)
                     /locus_tag="SI621_003523"
     CDS             complement(6000..6281)
                     /locus_tag="SI621_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEI4066191.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6285..6488)
                     /locus_tag="SI621_003524"
     CDS             complement(6285..6488)
                     /locus_tag="SI621_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HEI4066192.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6488..6997)
                     /locus_tag="SI621_003525"
     CDS             complement(6488..6997)
                     /locus_tag="SI621_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HEI4066193.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(7097..7840)
                     /locus_tag="SI621_003526"
     CDS             complement(7097..7840)
                     /locus_tag="SI621_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HEI4066194.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVRLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7844..8917)
                     /locus_tag="SI621_003527"
     CDS             complement(7844..8917)
                     /locus_tag="SI621_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HEI4066195.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(8976..9830)
                     /locus_tag="SI621_003528"
     CDS             complement(8976..9830)
                     /locus_tag="SI621_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HEI4066196.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTNRADETSQAFTRLKNSLDSTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            10004..11776
                     /locus_tag="SI621_003529"
     CDS             10004..11776
                     /locus_tag="SI621_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HEI4066197.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFVANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11776..12810
                     /locus_tag="SI621_003530"
     CDS             11776..12810
                     /locus_tag="SI621_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEI4066198.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFLPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKIIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEVLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2813 a         3852 c         3599 g         2941 t
ORIGIN      
        1 acaccaacag gcaatgcaga gccttctccc aaaccaagat aatcaagaac tccctgtgtg
       61 ctgggcttac taagaattgc acgaccaaca cttgtcagtg cagttaacgc ggcacgatct
      121 acccctgtaa aataagggag tttatctgcc gctgtagcaa gccctgccag agcagtgagt
      181 atggcgtttg ccggttgtct tccattagcc aggtcgtatg cagcctttac cgcttttggc
      241 gttgcagcca gtgtttcaga tgtgctgttg gttgcactac tgagctgaac aagccctttt
      301 tgcgtcgtgg tggcgttctt ggcggtatat ttcccgttag ccaggtcata cgccgccttg
      361 accgcttttg gcgttgcagc cagtgtttca gacgtgctgt ttgtggcgct actgagttga
      421 acaaggcctt ttcgtgccgt agtcgcatcc tgagcggtat atttcccgtt agccaggtca
      481 tatgcagcct ttaccgcttt cggtgttgcg gccagtgttt cagatgtact gttagttgca
      541 ctgcttaact gagtaaaacc ttttgcggtc agcgaggcgt ccgggtggcg tcgtgactgt
      601 tcgtgctcag aaattttgtc atccacatat ttacgtgttg ccagtaccac agccgggtcg
      661 attttcagcg tgatggcttc agtgttcgtg acaaccagaa tcatgcggat agtctgggta
      721 cgaccactgc cttcctgcaa ctgcggtttg tacgtttccg ggcagttcgc cacagcaatg
      781 agtacgcctt catcatcata aagtccaatc tcacggatcc agaatcctcc ctcgttttca
      841 gggatgattt gctccgcaat aatctggctc tgattgttcg ggtcaacact cagaagattc
      901 agcggtgcaa tgcgtttctg gttaatcagt tttgtctgtg ccgggtctgg tgtcggcaag
      961 acaccattcg catcaccaac ggccatttgc gtcagattca gcttactgcc gagcatcgtc
     1021 gcgttagcca gccgtgctgc gccctgatta gtcagaatgg cgtagtattt cactgtcatg
     1081 cgtttactct caggttatca attaaatgaa tggccgaggc ctggaaataa tcccctccga
     1141 caataatggc ctccggggtg tagggataaa ccgtcagggc gtcaccgtga tagcatcccg
     1201 caccggcaaa aatgttgccg gttgtactta aactgatagc cagtcccgtc agatggcggc
     1261 ttgccggttt tgcatcagca acgaggcgct ccagctcctg atacatttcc tcggtaatac
     1321 cctgctcaag cacgccaaca acgatgcgga acgtccccgg ctcctcgttg agctgccacc
     1381 actccctcac ctcaatcaga tagccgagcg gctccaccac acgccggatt gcgcctatag
     1441 tgcccttatg gcagtgaatg aaatacgcat cgcggataag agcgcgtttt gttgcttccg
     1501 gccacttatc atcccagcgg tcaacagaaa acgcccacgc cagccacggc agcagatttg
     1561 ccggacaggt atccgggttc cacagctcac gaatactgac cggcgttttt tcaatttccg
     1621 cacaggcttt tgcggcggca acttcaagcg gtgatgagcc ggtcggcagc aggcgcgaat
     1681 cactcatccg agcctccggt cacgacgcgg tattcggtac agaaagacgc ctgcgtgctg
     1741 ttaagcacga tatcggccag cggtgcagcc agttcgacac gctgcacgcc ttccacatgc
     1801 aaagcggcat aaatggcaga cagacggatg tcgcgcccca gccggtgctg tgccgtgatg
     1861 tacgcttcca gttttttcac ggcagcagcg cggatgggtt cgctttcggg accagggtaa
     1921 aggtaaagcg tggcgtttat ctggtattca acgatggcag cagactgcac ggtcacgcgg
     1981 tcggccaccg gcctgacgtc ctcgccatta agggcgttac gcaccacggc cagcaggtct
     2041 tcggatgcga cgccgttatt ttcacgtgac agcacggaga tagtgacgca ggccggagac
     2101 ggactggtga cagagatatc cgcgacacgc ccgtcagcac tgcgaccatg atactgatag
     2161 gctcccaccg acccggcgac gcttaagccc tcaaacgcct gctgaatacg cagacgataa
     2221 tcggtgtcag actccatcac tgccggtgtc ggcgggatag tcgaatcatc tgccggggtg
     2281 ataatcaggc gcgtggtgtt gtaattggca ccaatcacat caaggtcatt accggcggca
     2341 caggccagca ttaccgcccg tgcggcctca ttcacacgct gacgccagat aagctcacga
     2401 taagcatttt cctccagcag tttgacgaga ggctcggatt ccagcatcag ggtacgggca
     2461 accgcctcct gctggtcttc cgggtaaagg gaaatcagtg tcgccttgcg ttcggcaaga
     2521 atggtttcaa agtccagctc ctcgaccaca tccggtgcgg gtagctggtt caggtcgata
     2581 atcggcatgg tttcaactca cagggatggt taacgaaagt ggctggccgg tgtcgttgtg
     2641 ctgaccggtt aacgtgactg tcattcgccc gtcaaaactg cgcgccgtgg tgacggatga
     2701 cagggtgacg cggggttccc atttcagcac ggccatgtaa caggcgacct taatctgcaa
     2761 ctcaagcgcc ggggtctgcg gctggtcaat cattgacgcc agcaacgagc cgtaatcacg
     2821 acgcatcacc cgtgagccga ccggcgtgcg caggatatcg ccgatactct ggctgatatg
     2881 ctcaaggtca gtgacagtca ggccatcact gcgattcatt ccgagataac gcgctgtcat
     2941 agaggactcc cggttgtgcc gccgctgtcg ccggggtgtt tatgggtatg cagtacctta
     3001 ccgtttgatg agagttcacc gccggtgtgt tcaatgttgc cgcgcatcgt cccgcccttc
     3061 tgcacttcca gcgtgccggt aatcagcctg ttggtgcaga ccacctccgg tgtgtccagg
     3121 gtgatgcggg ttgacgcttt caccatgacc accggcaccg tggcagtaac agaatcagaa
     3181 gccgtcacgc tggccgtttt aattccgctt accgtgagtg cactggtttc gggttcatac
     3241 tcaatcaccg ccccgtcagg gaaacggata tgcagggcat ccgccgacgc agacggcgca
     3301 gggttatcgc cggaataaat ccccggcaga acgaacgccg tgtcgagttc accgcccacg
     3361 gccagaatca gcacctgttc ccccacggaa ggtgcccacc atgtacgcga acgtccggca
     3421 cgatgggtca gccactgaag ccagtcggtg cacatgccgc cggtctgcac acggcagcga
     3481 ccggcgttaa ggtcggtttc gacgataatg ccggtgcgga tcatgttgcg cagtgcgcgc
     3541 gcgagttcct gaatatttgc gagagtgttc ataacgggaa ggatgccgcc gggtcatacc
     3601 ggcggcaatg tgacgatgag gtgtcgggaa tggcacaact aacggtcgag gtgagccaga
     3661 ataatctctt caatcatctg cacatcctca ccggtgaagc cgagcagagg gcgcgccgga
     3721 taatcaattt tcttaccgtc tttccggttt tcttccgaca gaccaaactg atgcacactg
     3781 gcgattttcg gtgactttcc gccgtaaaat tccattgatg cctgctccgg gctggcgcgg
     3841 atatgcaaaa aacgactggt gataagtttc gcaaacattt ttcgcttaac gcgaccggtc
     3901 ttttttctgg cgctctgctg ctggcgtggc gcgtagggtg tgccgtccgg ggctttctgt
     3961 gccatcaccc gacgctgctg actctgacgc agacgtttcg ccagttcggc gctcagtcgt
     4021 cgacgccctg acggtgacag cgattcaatc agtccggtca gccggtcttc aaaacgctta
     4081 aactcattca tcccacttgc tcaccagttc gccattgata taaagctcca tcgggcgggt
     4141 gaccggctcc ggcggcgggg gttccgggat attcttcaca tgcagtgcgc cgtccacctc
     4201 actgaccagc gtgcgctcgg tcagcatcag gctgatgctg atatcaaagc tgctgtcatt
     4261 gttgatgtct gcataaaacg tgaagccctt tttctggcct tcgtcggtgg tcatgatgtc
     4321 gggctgattt tcccgcagcc acgccagcac cggcacgatg agcaggtcaa aatcaccggt
     4381 aaagtcggtc acaatgacat tgagcgtgta acgcttttcg aatgacaacg acgtcgccag
     4441 tgtggaggca atactcccgt tatccacgaa tatccgcagc atctcgggac tggttttcag
     4501 caccgtgacg gcatcagtca gcgccctgcg caggctgtcg ggtttgagca tcgttttcgt
     4561 cctgacagtg tttaatcatt tttacctggc tggcacagcg tgccagcgcg ttctcaagct
     4621 gccggatatc ggcacttaaa tcgccgttcg tctccgggtc actgcccggc atcgggcaaa
     4681 ggctcacttt cgggcaggcg ttgtggacaa tcactggcgt cggtgcaggc cgggcgctgg
     4741 tgcaaccggc gcacagcatc aggcaggcca gcgccgtacc agcggcgaaa atcttcgttt
     4801 tcattgagta acctcgtgat ggttttctcg cgctgtgctt cacgcttcgc ggcgttttcc
     4861 agttcctgac gcagtgccac ctgcgccagc tcgtttttgt ctgccctggt gagtgcaaca
     4921 tgaagctggt ttttcagcat ggtgatggtc gtctgctgtt cactggcgac gttgttcacc
     4981 ctgtccagcg aggcgcgcag gctggcgttt tcatgcttcg ccagaaacag ccccgccacc
     5041 gccagcgata acaacacaac cagcacaatc atcagctttg acatagttcc cgcccctcaa
     5101 gacgctgacg gcaggcagtc tgcatcagcc ggaaaaacag cgatgccacg agataaatca
     5161 gcgcggtaaa aatccacccg gcagcgacca gcgagataaa cgtcaccacc agacccgccg
     5221 cccgtctgca ccacgtcacc ggctgcaaaa acagcgccgt gacaatctgc acatccagag
     5281 attctggcgg cagctcgcgc ccgtagcgtt ccagcacata ctcagtggca tacacgccga
     5341 caccaccggc aaccacacag ataaccgtcg ccagaatcgc ccaggcggcg acaaaattga
     5401 cggccacgct ctgcgggtaa atcagggaca gtgccagcat cagcgccagc gacacgttca
     5461 gcatccgtga aagggataat tttttcatgg tgtttactcc gtttaagccg gtacgccgcc
     5521 agcggtacgc cagacggtga ccagtttttc cagtgaatgc tcacgctgac cgtaaccggc
     5581 ccccggcagg gacgcccaga tattgcgaca gcgtgaaatg gcgcgctcaa tgcgtcccgc
     5641 ccggatgtca tccagtgcac cgcgttcgcg gatcaactga atggcgagtc tgtcctgcga
     5701 caacggactg aaatccggca gggcaagctg tttgcggtag tgcggccaga acaggtaaag
     5761 ctgctgatag cgaccggagg ccgtggattt ttcaccgcga cggttaaaca ccttcgccgg
     5821 tcggccatgt gcgaacgggt ggtcactgta gtcggtgaag atttccggct tcccgtccag
     5881 tccggtgact atcacgtcat agccccggtt tttcgtcagc ggatgattcg ccgtcccttc
     5941 ggacacggcc agcatgtcga gaaaggcagc gatattctga tgcgtattga ttaccggcat
     6001 tacggtttcc ccctgccctt aaagcggcgc tgaatggcaa tctcaatcac ctgataaccg
     6061 gcgataccca gcatggagcc gatgccgcac accgcaggca gtgacaggtc aggaaactgc
     6121 accagaacaa caccggcaac catcgagaca aaaccaccga gcaacatgcg cccgataaac
     6181 agacgcgggg tgatgggttc accaccggca agcaccttgc cgacaacaat cagcacccca
     6241 atcatgaaaa gcgacaggac gcttttttct tctgctgtca tgcgttactc ccacagattg
     6301 acagtttcag ccacgggcgc ggtctgaacg tcgggcagtt cgacggcggt gccgtgcggc
     6361 agcaccgcac ccagttcagc cagtcccgga tttgcggcga gcacggcctc aaccacgccc
     6421 tcagtgcgcc cgtaataccg gacacaaatg gcgtcgagcg tgtcgccctg tagcgcaaag
     6481 gtcttcatca gatttgactc acgatgcagc gcggcttgtc ctggatacgc gccaccgccc
     6541 agcgcatatc ccgccacagt tcatcaatgg tgctgtcaat gctgtcagcc ttcttgtcgc
     6601 ctttcgcact ggcatccacg ccgcgataac gctcataaag cgacgcggtc gccatcgcac
     6661 acacggcgcg ctcgtagtaa aaaactttga tactttcacc gtcgatgtcg tccgccggaa
     6721 cgtccgccag acgcgtaaaa ccggcggcaa ttttctgttc gcggtactcg tacagctccg
     6781 catttgtttc agccatgcct gacttgatgg cctcacgcag acgggcgggg gcgacggtct
     6841 gctcaaggcg catacgttcc cggacgcgct tcgggtcgat atcgggaaaa aagaacgtgt
     6901 ttttaatcac cggctcgtcg cctgccggtt gcgggatgac caccgtaccc tcaccggaca
     6961 cgggggcctc ctttcgcgga ataatcagcg tcatcatgac tacctctgaa aagtcgggcg
     7021 gtggacgccg gtgcagtgtc aggtgattca ccctcactga ccggcgtgcc gccctggcgc
     7081 ggggcgcatt cggttgttaa ctggctttct ttttcgggcg tccacgtttt gccggtgtca
     7141 cgctccgggt cttacgcggg gcgcgggtgg ccgctttggg ctgcggctcc ggcttcggtt
     7201 tcagctcccg ctccagtcgt tcaatctctt ttttgacgcc tgcctgacag tcgagctgtg
     7261 tcgcacgttg caggtgagcc agcgcaccgg cggcatcacc agcgtcacgc agaaacagac
     7321 cggtgatttt gtgcagcttt gcgcgcactt catcaggcat gtctgccgtg gcggtcagtg
     7381 caagggtctc cgtcagcagg cgggtatcca cagactcacc ggcagcgtgg gcgcgcatgg
     7441 ccgcgagcgc cacctcctcg gtgaacatgt acggcggggt gcggcggtgt ttacccggca
     7501 tggtcagacc gtacttcagg gcataacggg caatctccag cgcaccggca atatcgccgg
     7561 tatccagacg ccacagcatg accgtcatca gaatgtcatc ctgtgcacct ttgccctgct
     7621 ccagcacgcc gttcacccac ggcaaccaga acggcagcag ttcgcgtttt ttcgcggcct
     7681 tcagctcttt tgaataaatc gctttcagtg tgcgctggtc tgcggccagc ctgaccagca
     7741 tctgctcata gacagttgca tgtcgcagcg gggcggcgtc ccgctgcgcg gtcatcgctg
     7801 ccgagacccg catcatgtgg cgctgtgcgg gactcgtcat cggttacgct cccggctctg
     7861 cggtcacttt agtcggtgtg gagaagtcac cgaccttaat tttttccacc agacaaccgg
     7921 cggcgtagtc ttccaccacg taatcaatgt tcattgactc gtagttctcc acgcggtcga
     7981 gtttcgggtt ttccacaatc acgcggcgat ggctgtcatc catgtagtag atggacaggt
     8041 tttccagctt cgtgatgagc atcgcatccg ccgggaagta cgggacgcgt accgccggca
     8101 ggttaccgat gcgtttctgg ctgatgatga cgtcagcggc cagcatctcg ctgttgtcct
     8161 gctccttgtt gacgatgggg aaatacttgt ccgccagtag ctgacgcccc acaatcacca
     8221 caaggtcagg gtcttcctga taccacggct caatcaggtt gttggtcgca tccatcacca
     8281 gtgcatcgag gctggcataa tcaccgccct tacccacgcg gataacctca gaggtcgtgt
     8341 gaccttcctc gtcagtaacc ttactcatca cgcgcgccgg ggcttcattg cggtatttct
     8401 gcagccagcc gaccgccaca tcctgcagca tctggttact gctgcggtca gaggtttcgg
     8461 cacgcctcac gccgttaaaa ccggccatga ttaaatcaag ggactggcgt ttgataatgg
     8521 cgttacggac acggagctgg aaatcctgat aacgcgccca caggtcaagc gttttgtagc
     8581 ggatataaaa atcgaagtta atctggtcgc attcgtactt gtttgacgcc agcttcgaga
     8641 agtccttcgg ctgacgctcg gtgccaccgg cggtgtctgt ggtgctggcg atggagccgg
     8701 tgacaccaat accaattttt tcccctttca tttcgctgac cggcacaatg ttgatgcggg
     8761 tcagaaagtc agaggactcc tgcatggtgt tcatcagggt ctgggtgacc gacggttcaa
     8821 cggtgaattt tttcgacaca tcaccggcgt cgatgccgtt cagttcggca acacgggaca
     8881 ggtaggcatt aaatttaaag cgggtttcct ggcgcatatt ttttcctgaa attaagggtt
     8941 aatcgtgaag gtttgcccgg actggctgac gccggtcagc agttcgtcat cagggcgtca
     9001 ccaccaccgc cggtggcttt gctgcggcgc tgctgggtca gactttcggt gctgtcgaga
     9061 ctgtttttca ggcgggtgaa tgcctggctg gtttcatccg ccctgttagt cacatcctgc
     9121 ttaagtgcag aaaaggcggt ttccatctct gcgaggcgct gctcagtggc gctcagtttt
     9181 tcctgcacat gttcagcgac agcggtcacc gcttcatgca catcattcag acgggcgtca
     9241 tcgctggcct gtttgcggcc aaaaatggat ttcacctttt cggtcagggc ggtgaacacg
     9301 gtttcaggca ggtcttcaaa ttccagctca acaggcgttg ccactgaaat cagattttca
     9361 gggcttaatt tgaagcggtt cagggggttg tgttttgccg tgcggcagaa ttccaggtat
     9421 tccgtgccga ggcttgccgg gtcatcggtg acggccagcc ccaccagata acatttgccg
     9481 gtgttggcaa agttcggctg aatttccatt gaggtgtaga ccttctgcgc ggccttgttc
     9541 atcgcgataa ggtcatcggt cggggtgatt ttcgcaaaca gcgcccattt gcctttcagc
     9601 gccgaatcat cgtcaatctt ttcggccttc agttcaacca catcgccata acgtttaaaa
     9661 ataccgtcag gcaggatgcc gcgcagatgt tccaggttaa tacggcaacc atagactcgc
     9721 gggtcaaagg tttcggccat ttcctgaata tcctgcgcac tgatgacacg cccgtcacag
     9781 gtgtcaccct caacgccgat acgaaagaat tttgagactt tttttgccat tgtcaggagt
     9841 cctgaatagt gattagagga gtcacatgtc ggcatcagtt tcccgacgat gcgcatcctc
     9901 cgccatcagt cccggatggc ttatcactga cacaacagca ccttagcgaa tcgcggggcg
     9961 cgactcagta gccttgccgt gtattcatca cggcgaggta ttcatgacca tcaccacaga
    10021 caccactctt ttgcacgacc cgcgtcgtca ggcggcgctg ctgtactggc aggggttttc
    10081 cgtgccgcag attgccgcca tgttgcagat gaaacgcccg acggtgcaga gctggaaaca
    10141 gcgcgacggc tgggacagcg ttgcccccat cagccgtgtc gaaatgagtc tggaagcgcg
    10201 gctgacccag ctcatcatca aaccgcagaa aaccggcggt gacttcaagg aaattgacct
    10261 gctgggacgc cagattgaac gactggcacg ggtcaaccgt tacagtcaga ccggcaacga
    10321 ggcagacctt aatccgaacg tcgctaaccg caacaaaggc gggcgccgca aaccgaaaaa
    10381 gaattttttc agcgacgaag ccatcgaaaa gctggagcag attttctttg agcagtcttt
    10441 cgaatatcag ttgcactggt atcgcgccgg gcttgagcac cgcatccgcg atatcctgaa
    10501 atcccgccag attggcgcaa cgttttattt ttcccgcgag gcgctgctgc gcgccctgaa
    10561 aaccggccat aaccagattt ttctgtcggc cagtaaaacg caggcgtatg tgttccgcga
    10621 atacatcatc gcctttgccc gtctggttga cgttgacctg accggtgacc cgattgtcct
    10681 gggcaataac ggcgcaaaac tgatttttct cggcaccaac tccaacaccg cgcagagcca
    10741 taacggcgac ctgtacgtcg atgagatttt ctggatcccg aattttcagg tactgcgtaa
    10801 ggtggcatca ggtatggcct cacagagtca cctgcgttcg acctatttct ccaccccgtc
    10861 cacgctggcg cacgacgcct acccgttctg gtcgggtgaa ctgtttaacc ggggacgcgc
    10921 cagcgccgct gaacgcgtgg aaatcgacgt cagtcataac gcacttgccg gaggtcttct
    10981 ctgtgcggac ggccagtggc ggcagattgt caccattgag gacgccctga aaggcggctg
    11041 cacgctgttc gacattgagc agctcaaacg cgaaaacagc gccgacgatt ttaaaaacct
    11101 gttcatgtgt gaatttgttg acgacaaggc gtcggtgttc ccgttcgagg agctgcaacg
    11161 ctgcatggtc gacacgctgg aagaatggga agactatgcg ccgtttgtcg cgaatccgtt
    11221 cggctcccgc ccggtatgga ttggttacga cccgtcacac cgtggcgaca gcgccggatg
    11281 cgtggtactg gcaccgccgg tggtggccgg tggcaaattc agaatacttg agcgtcacca
    11341 gtggaaaggc atggactttg ccacccaggc ggaatccatc cgcaaactca ccgaaaaata
    11401 caacgtcgaa tacatcggta ttgatgccac cggccttggt gtcggcgtgt tccagctcgt
    11461 gcgctcgttc tatcccgccg cgcgcgatat ccgctacacg ccggaaatga aaaccgcaat
    11521 ggtgctcaag gcaaaagacg ttatccgccg tggctgtctg gaatatgacg tcagcgccac
    11581 cgacatcacc agctcgttta tggctatccg caagaccatg accagcagcg gacgcagcgc
    11641 cacctatgag gccagccgca gcgaggaagc cagccacgcc gacctcgcct gggcgaccat
    11701 gcacgccctg ttaaatgagc cactcaccgc cggtatcagc accccgctga catccaccat
    11761 tctggagttt tactgatgag caagaaaaaa gggaaaacac cgcaacctgc ggcaaaaaaa
    11821 atgaccgcca gcgccccgaa aatggaagca ttcacctttg gtgagccggt gccggtactc
    11881 gaccgccgtg acattctgga ttacgtcgag tgcatcagta acggcagatg gtatgagcca
    11941 ccggtcagct ttaccggtct ggcaaaaagc ctgcgtgctg ccgtgcatca cagctcaccg
    12001 atttacgtca aacgcaatat tctggcctcg acatttctcc cgcacccgtg gctttcccag
    12061 caggatttca gccgctttgt gctggatttt ctggtgttcg gtaatgcgtt tctggaaaag
    12121 cgttacagca ccaccggtaa gatcatcaga ctggaaacct caccggcaaa atatacccgc
    12181 cgtggcgtgg aggaggatgt ttactggtgg gtgccgtcct tcaacgagcc gacacctttc
    12241 gcgcccggct ccgtatttca cctgctggag ccggatatta atcaggagct gtacggcctg
    12301 ccggaatatc tcagcgccct taactctgcc tggctgaatg agtcggccac gctgttccgc
    12361 cgcaagtatt acgaaaacgg cgcacatgcc ggatacatca tgtacgtcac tgatgccgtg
    12421 caggatcgca acgatatcga agtgctccgc gaaaacatgg tgaagtcgaa aggccgcaac
    12481 aactttaaaa atctgtttct ctatgccccg caggggaaag ccgacggcat taaaattatc
    12541 ccgctcagtg aagtggcaac gaaggacgat ttttttaata tcaaaaaagc cagcgccgct
    12601 gacctgctgg acgcgcaccg catccccttt cagttgatgg gcggcaagcc ggagaacgtc
    12661 gggtcgctgg gtgatattga gaaagtggca aaggtctttg tccgcaatga acttatcccg
    12721 ttacaggaca ggattcggga aataaacggc tggctcggcc aggaggtcat ccgctttaaa
    12781 aactactcac tggacactga caacggctga acatcgccgc ctgcgggcgg cttttttaca
    12841 ccccgtcatc acgccctcac acgctcacca ccgcacaaaa caccccgcag acacaccaac
    12901 gccccggcgc acaatctaaa cgccatcaag acgcgctcag acgctgaaaa aataaaatca
    12961 gcaccaccgc cagcgcgcag tgctttcccc gcctcgcccg cccgcttcat gtggaggttt
    13021 taatgcggtt gcatgacatc tctgaatcca aactagctgt gatgtcggac gccgaaaacg
    13081 tataattctg atacatgcaa agccatgcac ctaatgcatg cataactctg atcttatgaa
    13141 atgacataaa gcagcatcac agaaggtgac tttaaagctt tgtgttgtca ctggaaggaa
    13201 atgaa
//
LOCUS       DARNVM010000134        13170 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.134, whole genome shotgun sequence.
ACCESSION   DARNVM010000134 DARNVM010000000
VERSION     DARNVM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.134"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..632
                     /locus_tag="SI621_003531"
     CDS             <1..632
                     /locus_tag="SI621_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4066199.1"
                     /translation="SLIIGAVFDYSNLSDKNLSDKDLTNISCMYTNFSNANLTKCKLF
                     NTNFSAAKFDNTNFTGTEGSNILFNHAWLFNTIFIDTIFKNACFFNAKVNNVSLKKAY
                     LYNDNSNKKANDSTEKQANDSTDKQANDSIEKQASDSTEKQASDSIEKQANDSTDKQA
                     KNSTDKQAKNSTEQQDSTSLNQARLKKEVNSSFSIPGLTSYQPTYIVDE"
     gene            888..1730
                     /locus_tag="SI621_003532"
     CDS             888..1730
                     /locus_tag="SI621_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HEI4066200.1"
                     /translation="MKKRITFIVFLCAIVAVSLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNTESEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTMTGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1798..2976
                     /locus_tag="SI621_003533"
     CDS             1798..2976
                     /locus_tag="SI621_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066201.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFTFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHIIAPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3187..8427
                     /locus_tag="SI621_003534"
     CDS             3187..8427
                     /locus_tag="SI621_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4066202.1"
                     /translation="MSSTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSPSPLIENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTLQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTPSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSIQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYTQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYTANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDAYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTATVTDFHNNPLKDMKVNFVAPGGSQLDNTSATTDQSGIVRVHLTSSKA
                     GSYSVDASLEADKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNTVILTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAHQQVTFIGDTNSAQLQPLTASLNTIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTAHITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNAQDELMPGQIITFSVTPEGATLSNTGEILTDQYGQAKVTLTSDKVNVYTVTA
                     TMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTVTSRVEDDYGFPVE
                     GVDVRYTLDTKGRPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSAAIT
                     LLASTADESKSLLKSDVGTLMADYQHSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIRSIKEMSG
                     HVTANNHTFSTAQFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTQGAYWTSESDDYNRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(8548..9132)
                     /gene="kptA"
                     /locus_tag="SI621_003535"
     CDS             complement(8548..9132)
                     /gene="kptA"
                     /locus_tag="SI621_003535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEI4066203.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEWPFMPAGVQKQ"
     gene            9382..10065
                     /locus_tag="SI621_003536"
     CDS             9382..10065
                     /locus_tag="SI621_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066204.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            10062..10523
                     /locus_tag="SI621_003537"
     CDS             10062..10523
                     /locus_tag="SI621_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HEI4066205.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10536..11708
                     /gene="iadA"
                     /locus_tag="SI621_003538"
     CDS             10536..11708
                     /gene="iadA"
                     /locus_tag="SI621_003538"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEI4066206.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGVCDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFAHKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11773..12684
                     /gene="hypT"
                     /locus_tag="SI621_003539"
                     /gene_synonym="qseD"
     CDS             11773..12684
                     /gene="hypT"
                     /locus_tag="SI621_003539"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HEI4066207.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     KLSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12677..13069)
                     /gene="iraD"
                     /locus_tag="SI621_003540"
     CDS             complement(12677..13069)
                     /gene="iraD"
                     /locus_tag="SI621_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HEI4066208.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(13066..13149)
                     /gene="idlP"
                     /locus_tag="SI621_003541"
     CDS             complement(13066..13149)
                     /gene="idlP"
                     /locus_tag="SI621_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HEI4066209.1"
                     /translation="MENEHQYSGARHSGQAAYVAKRQECAK"
BASE COUNT         3436 a         3281 c         3191 g         3262 t
ORIGIN      
        1 cgtcattaat tattggagca gtatttgatt attctaatct atcagataaa aatctttcag
       61 acaaagatct tactaatatt agctgcatgt ataccaattt ttcaaacgct aatttaacaa
      121 aatgcaaact ctttaataca aacttttcgg ctgcaaaatt tgataatact aatttcactg
      181 gtacagaagg ttcgaatatt ctatttaacc atgcatggtt gttcaatacg atatttatag
      241 atacgatttt taaaaatgcc tgttttttca atgccaaagt gaataatgtt tctcttaaga
      301 aagcatatct ttacaatgat aatagcaata aaaaagccaa tgacagtacc gagaaacaag
      361 ccaatgacag taccgataaa caagccaatg acagtatcga gaaacaagcc agtgacagta
      421 ccgagaaaca agccagtgac agtatcgaga aacaagccaa tgacagtact gataaacaag
      481 ccaagaacag taccgataaa caagccaaga acagtaccga gcaacaggac agtaccagtt
      541 taaatcaagc ccgtttaaag aaagaagtga atagcagttt ttccattccg ggtttaacgt
      601 cttatcagcc aacgtatata gttgacgaat agttcaataa tcacccaaac agagtgagta
      661 tggcagagga tataaattgc taaccgcctc atctatcatt catggatgag gcgcatttat
      721 cagtcgatgt aagctgaaaa tgttccgcca tccagagaca accgccgccc cctgcggcgt
      781 ttactatttc atcgcccgcg ttaagaattc attaagcttc atccgtatac tccttatctg
      841 tcgcactgaa tttgacgaga aaaaaagcaa taataaggag caacaagttg aaaaagcgca
      901 tcacctttat cgtttttctt tgcgccatcg tggcggtgag cctgtttttt gtgcaatcct
      961 gcgtccgtaa aagccagcat gtcgcagggt ttcagaatta tcaggccact atcgatggta
     1021 aagagattac aggagtaact aaaaatatct cctcactgac ctggtcagcg cagagcaata
     1081 ccttgtttag cactatcaac aaacctgcaa ccatcgttga aatgacgacc gagggtgacc
     1141 tgatccgcac cattccgctc gattttgtta aggatctcga gacgattgaa tacattggtg
     1201 ataataaatt tgtcatcagt gatgaacgtg actacgccat ttatgtgatt tcacttaaca
     1261 ctgagtcgga agtaagcatc ctcaaaaaaa ttaaaatccc attgcaggaa acaccaacta
     1321 actgtggctt tgaagggctg gcttactcct ctcaggacca taccttctgg ttttttaaag
     1381 agaaaaatcc gatagaggtc tacaaagtaa ccgggctatt gcgtagcgat gagttgcata
     1441 tcagcaaaga taaagcgttg cagcgtcagt ttactttgga tgatgtttct ggcgcggaat
     1501 ttaacccaca gaaaaatacg ttgctggtgt tgtcacatga gtcacgcgcc ctacaggaag
     1561 tgaccatgac aggtgacgtg atcggcgaaa tgtcgttaac caaaggcaag tatggccttt
     1621 cacacaacat caagcaggcg gaagggatag cgatggatga cagcggcaac atctatatcg
     1681 ttggcgaacc gaatcttttt tatcgcttta cgtcgacaaa atcacgataa cagtctggca
     1741 ggttctcctt ttttgttatt aacttgttaa ctcaaaataa tacaaaccag gcagaacatg
     1801 aattcgacat cgcatcccgt agaacgtttt tctttcagca ccgcgttatt cgggatgctg
     1861 gttctgacct taggtatggg tttaggccgc tttctctata cgccgatgct gccagtcatg
     1921 ctggcggaag gcgaatttac ctttagcgaa ctctcgtgga tcgccagcgg taactatgcc
     1981 ggatatctgg cggggagcct gctgttttca ttcggcgcgt ttcatttacc ctcacgcctg
     2041 cgcccgttct tgttagcttc cgctctcgca accggattat taatcctcgc gatggcgtgg
     2101 ctgccgccgt ttcttctggt tttcatcatt cgctttctgg cgggggtcgc cagcgccggg
     2161 atgttgattt tcggctcaac gctcatcatg caacataccc gccatccctt tgtccttgcg
     2221 gcgctatttt ctggtgttgg cgtcggcatc gctctgggca atgaatatgt gctggcaggc
     2281 ctgcattttg ccctctcttc acaaacgttg tggcaaggtg ccggagcact ttctgccatt
     2341 atattgcttg ctctggcgct gctcatcccg tcgaataaac acattatcgc gccagcgcca
     2401 ttggcaaaaa tcgcgcaaca acccatgagc tggtggttac tggcgatttt gtatggtctg
     2461 gcgggttttg gttatatcat cgtcgccacc tacctgccgc tcatggcgaa agacgcgggc
     2521 cagcctgtgc tgacggctca tctttggaca ctggttggct tgtcgattgt cccaggttgt
     2581 tttggctggc tgtgggcagc caaacggtgg ggagcattac cttgcctgac cgcgaatttg
     2641 ctggtgcagg cgatctgcgt gctgttaact ctcgccagca gctctccttt attactcatc
     2701 atcagcagta ttggttttgg cggcaccttt atggggacga cctcgctggt gatgaccatc
     2761 gcccgccagc ttagcgtgcc gggaaatctt aaccttttgg gctttgtgac actcatttat
     2821 ggtatcgggc aaattcttgg cccagcgctg accagcatgc tcggcaacgg aacgtcggcc
     2881 ctcgccagtg ccacgctctg cggcgcggcg gcgctattta tcgcagcatt aatctgcggg
     2941 atgcaaatat tcaaattgca tacgaattat tcttaagtaa tttaacgaat ttaacttttc
     3001 agtacatcac gtaaaataat taaaacatcc tttttctttg gaggtactca gccaccgccg
     3061 ttattgttcc tggtttagca attccacggc caattcatta tttctggtca cattatagtc
     3121 aggatcgttt tatatctccg tgctagaaaa aacaagttta attctgtttt ttcacggaag
     3181 gtcactatgt cttcaacaaa tgcgcatcaa ataaaaaata atgatcaaaa ctctttatgt
     3241 ggtctgggtg acaaaatacg ccgcctgacc gccggagttt gtttatttac ccaaattttt
     3301 ttccccgtca tggcgacggc acaaaatgtg gtacacgcta aacctcagac gacagtttca
     3361 tctccgtccc ctctaataga aaataatact gtgccttaca cgcttggtgc gctggaatca
     3421 gcgcaaagcg ttgctgatcg attcggtatt tcactggagg agcttcgtcg tcttaatcag
     3481 ttccgtactt ttgctcgcgg ctttgataac gtgcgccagg gtgaagagct ggatgttccg
     3541 gcaacaacct tgcagaaaag tcatgagcaa caaaatgccg taccgcctgc gaatggcgaa
     3601 aacacgctgg agaatcaaat agccagcact tcgcagcgag ttggcaccct gctttcacaa
     3661 gatatgaata gtgagcaggc cagcggcatg gcgcgtggtt gggcgtcttc agaagcctca
     3721 ggcgcgatga ctgattggtt aaataacttt ggcactgcga gaatctctct gggtgtggat
     3781 gaagatttca gcctgaaaaa ttcgcaattc gacttcctgc atccgtggta tgacacacct
     3841 gattatctgc tcttcagcca gcataccctt caccgaacag acgatcgtac ccagatcaac
     3901 accggtttgg gctggcgtca tttcaccccc agctggatgt caggcatcaa cctgtttttt
     3961 gaccacgacc tgagccgcta tcactcccgc gcgggtcttg gcgcagaata ctggcgtgat
     4021 tatctgaagt tgagcagcaa cgcttatatc ggcctgactg gctggcgtag cgcaccggaa
     4081 ttggataatg actacgaagc ccgcccggcc aacggctggg atttacgcgc ggaaggctgg
     4141 ttaccagcct ggccacaact gggtggaaaa ctggtctatg aacaatacta tggtgatgaa
     4201 gtggcgctgt ttgacaagaa tgatcgccaa agtaatcccc atgctattac cgcgggcctc
     4261 aactataccc ccttcccgct tctgactctc agtgcggaac agcgtcaggg gaaacaaggt
     4321 gaaaatgaca cacgttttgc cgttgatctg acctggcaac ccagcagttc aatgcagaaa
     4381 cagcttaacc cggacgaagt ggccggacgg cgcagtctgg ccggtagtcg ttatgacctg
     4441 attgatcgca acaacaacat cgttctggaa tatcgcaaga aagagctgat tcgcctgagt
     4501 ctactggatc cggtgaaagg aaagtctgga gaaataaaac cgctggtttc ctcgatacag
     4561 accaaatatg ccctgaaagg ctataacatc gaagccgctg cgctggaagc tgccggaggt
     4621 aaagtcagca cgtctggaaa agatatcacg gtcacgctgc caggttaccg cttcactaac
     4681 actccagaaa ccgataatac atggtcgata gacgttaccg ccgaggatgt aaaaggtaac
     4741 ctgtcacgtc atgaacaaag catggtcgtc attcaggctc cgacattaag ccagaaagat
     4801 tctctgttat ccgttaatcc gctaaccgtg gctgcagata aaaaatcgac gaccacattg
     4861 accgttactg cgcacgattc cgacggaact ccggtgccgg ggctggcgct gcaaacccgc
     4921 agtgaaggcg ttcaggatat caccctgtct gactggacag ataacggtga tggtagttac
     4981 acacagatgc tgaccgccgg aacgacatca ggttcagtaa cactgacgcc gcaaattaac
     5041 ggtgagagtg cggtaaaaga atccattgtc gttaatatcg tccctgttgt ctcatcccgc
     5101 gaccattcat caataacaat tgataacgta tcgtattatg ccggagacga catcaaggtt
     5161 agggtggaac tgaaagacga cagcaatcaa ccggttgcat atcaaaaaga ggaattggta
     5221 aaagccgtta ctgtcgaaaa cagcaaacct ggcgccacga ttgtctggca cgaagagcag
     5281 cctggcgttt ataccgcgaa ttatccggcc cataagcaag ggacagcact aagggcacaa
     5341 cttagccttc acaactggaa tgctccactg caatcacata tttataacat tgaggcaaac
     5401 cagaataagg ctcgcgttgc cacattatca gcgacaaata atgacgttta cgccgataaa
     5461 aagacattta ataccctcac gatcaacgtc actgatgaga gtgataatcc cctgacaaat
     5521 catcaggtta cctttaagaa tgaaaaagga agcgcggagt ttgtcgaacc gccgcagcaa
     5581 aatacggatg catatggtgt tgccacaata aacatggtaa gtcaggttgc ggaagaaaat
     5641 acgattagcg ccacgctgcc aaatggtttc tcacaacgga taattgcgaa attcgttagc
     5701 gattcgagta cgccaaaatt caaacaactg gttgccgatc cagataccat tattgctggc
     5761 aacagccagg gcagtactct gaccgccacc gtcacagact ttcataacaa cccgttaaaa
     5821 gatatgaaag tgaattttgt ggcacctggt ggctcgcaac tggacaacac gtccgccaca
     5881 acagaccagt ccggtattgt gcgagtgcac ctgaccagtt caaaagctgg tagctattcc
     5941 gtcgatgcct cgcttgaggc ggataaaaat attcaccagt cggtcacgat caccgtggtc
     6001 ccaaacaggg aacaatcggt aatgaccttg aatgccgggt cgggcagtgc gatcgctaac
     6061 aatacaaata ccgttatcct gacagccagt gtgaaagatg tttacggaca cccgttgccg
     6121 gatgaggatg tgaaatttac cttgccagcc tctatgaccg ggaacttcac gctaagtagt
     6181 gaaaccgccc gcaccgatgc aaacggcgat gccgtggtca cattgcgtgg cacaaaagcg
     6241 ggtgagttta cagttacggc gacgctgacc agaaataata ccgttgctca tcagcaagtc
     6301 acttttattg gggatacaaa cagtgcgcag ctccagccgc tgactgcctc attaaatacc
     6361 attgttgcgg gtaacagtac ggggagtacc ctgactgcaa cgatcctgga cgcttaccaa
     6421 aatccgctta aagaccagct ggtcactttc cagagtaacg atgtcactct aagtggaaca
     6481 gaagtcacca ccaatacact gggtcaggcg acggtaacaa tgaccagcaa tattgccggg
     6541 caacataacg tcgtggtgag ccggaaagcg caagcctccg ataataaaac gtttagttta
     6601 tcagtgctgc cggatgaaag ttcggcgaag gtaataagta taaccggagc cgaaaaaacg
     6661 ataacggttg gcgaaaacat cacgctacgg atactcgtcc aggacgcgtt taacaatgta
     6721 atagcgggtc aacgcgtcag attaagtgcg cagccaacag ctcacattac gataggcgat
     6781 acggcttaca ccgataataa cggttacgcg tacgttaacc ttctcagcac ccagcctggg
     6841 gtttatcagg tgacggcaac gctggacaat aacagtagta gtaaggttga tgtgaatgtg
     6901 gcaaatggca aactcgagtt aacatcatcg aagccagaaa ccacggtcca taacagtgaa
     6961 ggtattacgc tgaccgcaac ggcgagaaat gctcaggatg aattaatgcc tggacaaatt
     7021 atcaccttta gcgtaacgcc tgaaggtgca acgctaagca atacagggga aatacttact
     7081 gaccagtatg gtcaggccaa agtgacgctg accagtgaca aagtgaatgt ctataccgtt
     7141 acggccacaa tgggcaaaga tgttcccgtt cagagccagg taacggttgc ggttaaggca
     7201 gatgctaaaa cggcacatgt ggtgagcgtc gtggcttctc ctgacaccat aaccgccgac
     7261 ggcatcgata gcagcaccgt cacatcacga gtagaagatg attacggatt cccggttgaa
     7321 ggtgtcgatg ttcgttacac cttagacacc aaaggtcgcc cggtagttaa tattccaact
     7381 acgcgtaccg atcagtccgg gcaagtcacg gcgacaataa ccagtacatt ggcagaaacc
     7441 ttaacggtca atgtgcaagt tcctggtaca gccaaccaat ccgcagctat taccttgctt
     7501 gccagcacgg cggatgaaag taagtcatta ttgaaatccg acgttggcac tctgatggct
     7561 gactaccagc atagcgcgaa acttacgcta acattgcaag acaagtacgg taacccgata
     7621 gtgacgtctg atcatctgga atttgtccag tcaggtccct tcgtgaactt tctcaagttg
     7681 agcgatattg attacagcca aagaaattat ggcgagtaca ccgtgactgt cactggcgga
     7741 aaagagggaa cagcgactct cattcccatg ctgaacgggg ttcatcaggc aaacttaagc
     7801 gtatcgctga atctcatccg ctcgataaaa gaaatgtccg gtcatgtcac tgcaaacaac
     7861 cataccttct ccacggctca attcccgagc gaaggttttg caggagcgta ttacacactc
     7921 aacaatgata actttgaagc gggtaaaacc gttgatgatt atatgttttc aagttcacag
     7981 agttgggtgt cggtcgatgc ttcaggtaag gtttctttcg caaatatcgg cgatcaaacg
     8041 tcagtcacaa taagcgctgt tccccgacaa ggaggtacaa cctaccagac cttaattaag
     8101 ctgaaaggct ggtgggtgaa taatggaaat cataccaata tctggctagc tgccaatgcg
     8161 ctctgtcatg ctaaaaatga tggatataat cttcctggca tcacacattt gacgtctggc
     8221 gaaaacaaac gcacgcaggg atcactgtat ggtgaatggg ggaacgttgg agcgttttcc
     8281 agtaattcgc aatttacaca aggagcttac tggacaagtg aatctgatga ttacaatcgg
     8341 cactactatg tgcagatgct aacgggtatg actggaagcg acgctgattc cagcccccaa
     8401 ctgaccgcct gccgtaaatc actttaattt tagtaggtta ttaaggatac ccttgtgcga
     8461 gggtatcctt tccgactgat aacaaacgct acattacctg atgcgctgcg cttatcaggc
     8521 ctatataacc cctgcaatat attgaattta ttgtttttgt accccagccg gcatgaacgg
     8581 ccactcaaga aactcaacag caaccgttga tgtcagccag accccgtttt ccgcttgcca
     8641 gaaagggagg cctcgtttcg ccatttcctg tgctttgacg gttaaaataa ccggcgatcc
     8701 gtgtcttgct cccactttgc gtgccgttgc ttcatcagca gagagatgta cgtagtgacg
     8761 ctcacctgca atcagcccct gtttttttat ctcgtcaagg aaacggcttg ccgtaccgtg
     8821 gtagagaaac tgtggcggtg ttttttcggc aaaggaaatg gcgacctgcg aagtcgaatg
     8881 cccctgaacc gcgcggatgc acctaccatc actggaataa ctaaaacgct ttttatcgct
     8941 ggtggcaacc acagtatcca gcaatgcacg ggttaaccgc ttgcccgctt tttgggcaca
     9001 aagtatgagc ttatcgatat ccgcccatcc ttcacggtcc agtacgatac caatcgcctc
     9061 cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta tccgccagct ctttttcgtt
     9121 gtactttgcc atctcgtttt cgtccctgga ttttctgttt ttatttactt atgtcattgt
     9181 cactgggttt atgagtttac tgcaactcaa tcctgcgcag agccttttcc acacagcgta
     9241 atttttccgc tatgcaaaaa acgcatagcc agaaacatac ttgcattttc cccataccca
     9301 catggctgat aaggtttttc cgctccagga attttcctgg ctcaagcaac acacaaacac
     9361 aaaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg ggagctggcg
     9421 acagaaaaag tgggcattaa agggtatctg gctttttttc tcaccataat ctttttttcc
     9481 ggtgtttttt ccgggacaga cagttggtgg cgcgtttttg atttttccgt gctgaacggg
     9541 tcgtttgggc aactgcctgg tgcgaacgga gccaccacct cgtttcgcgg cgcgggtggc
     9601 gcgggggcta aagatggctt tctctttgcg ctggagctgg caccgtcggt cattctctct
     9661 ctggggatta tttcaattac cgatggtctt ggcggattac gcgccgcgca gcagttaatg
     9721 acgccagtgc taaaaccgtt gttgggcatt ccagggatct gctcgctggc gctgatcgct
     9781 aacctgcaaa acaccgatgc cgccgccggt atgaccaaag agctggcgca ggaaggtgaa
     9841 attaccgaac gcgataaagt gattttcgcc gcatatcaaa ccagcggtag cgccattatc
     9901 accaactact tctcttccgg cgtcgcggtg tttgcctttc tgggcacgtc cgtaatcgta
     9961 cctttagcag tcatcctggt gtttaaattt gtcggagcca acattttgcg cgtctggctt
    10021 aacttcgaag aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact caggtccgca
    10081 aaaatgtcat ggatatgttt atcgatggcg cacgccgtgg ctttactatc gccaccacca
    10141 acctgctgcc aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa attaccggcc
    10201 tgctcgactg ggtggggcat atctgtgaac cggtaatggc gctgtgggga ttgccaggtg
    10261 aagcggcaac cgtcctttta gccgcattaa tgagtatggg tggagcagtc ggtgttgccg
    10321 ccagtctggc aaccgctggc gcattaaccg gtcacgatgt caccgtttta ctgcccgcga
    10381 tgtatttaat gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca gccgaagtga
    10441 atgccaaata ttacccgcat atcattacgg tctgcgtgat taacgcattg ctgtcgatct
    10501 gggttatgca gcttattgtt taacaaggag ttaccatgat tgattatacc gcagccggtt
    10561 ttaccctgct gcagggagcg catttgtatg cgccggaaga tcggggagtt tgcgatgtcc
    10621 tcgtcgctaa cggcaaaatt atcgccgttg ccagtaatat cccttctgac attgtaccgg
    10681 actgcacggt tgtcgatctc agcgggcaga tcctctgccc aggttttatt gatcaacacg
    10741 ttcatttgat tggcggtggc ggtgaagcag gtcccacgac gcgcacgccg gaagtggcgt
    10801 taagtcgtct gacggaagcg ggcgtcacgt cagtggttgg tctgctcggc actgactcta
    10861 tctctcgtca cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa gaaggcatca
    10921 gcgcctggat gctgaccggc gcttatcatg tcccttcccg cactattacg ggttccgtgg
    10981 aaaaagacgt ggcaattatc gatcgcgtga ttggtgtgaa atgcgccatc tctgatcacc
    11041 gttctgccgc acctgacgtt tatcacctgg ctaatatggc ggcggaatcc cgcgttggcg
    11101 gtttgctcgg cggcaaacct ggcgtcaccg tgttccacat gggcgacagc aaaaaggcgt
    11161 tacagcctgt ctatgacctg ctggaaaact gcgatgtgcc gatcagcaag ctgctgccga
    11221 cccacgttaa ccgcaacgtt ccgttgtttg agcaggcgct ggaatttgcg cacaaaggcg
    11281 gcactatcga catcaccagc agcattgacg aaccggtcgc ccctgccgaa ggtattgccc
    11341 gcgccgttca ggccggtatt ccgctggcac gcgtcaccct cagctccgac ggcaacggta
    11401 gccagccgtt cttcgatgac gaagggaatt taacccatat cggtgttgca ggttttgaaa
    11461 cgttgctgga aaccgtgcag gtgctggtca aagactatga tttcagtatc agcgatgccc
    11521 tgcgcccgct caccagtagc gtagccggtt tccttaacct gagcgggaaa ggtgaaattc
    11581 tgccaggcaa tgacgcagac ttactggtca tgacgccaga attgcgcatt gagcaggtat
    11641 acgctcgcgg caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga acgtttgaaa
    11701 cggcttaaac ggttgtcagt cgcccgaatg gacgtagaat gcccgcaagg gcggctgaca
    11761 gagtaaaacg taatggatga ctgtggtgcg gttttgcata atattgaaac aaaatggctt
    11821 tatgattttc tgaccctgga aaaatgccgc aacttttccc aggcggcagt cagtcgcaac
    11881 gtctcgcaac cggcattcag ccgccgcatc cgcgcgctgg aacaggcgat tggcgttgaa
    11941 ttgtttaacc gccaggtgac gccgctgcaa ctctcggaac aagggaaaat ctttcattcg
    12001 cagatccgcc atctgttgca acagttagaa agcaacctgg cagagctgcg tggcggcagc
    12061 gattacgcgc aacgtaaaat caagattgcc gctgcacact ctctttccct cggtctgtta
    12121 ccgtccatta tcagccagat gccgccgctc tttacctggg cgattgaagc cattgatgtc
    12181 gatgaagcgg tcgataaact gcgtgaaggg caaagtgact gtattttttc ctttcacgac
    12241 gaagatttgc tggaagcacc gtttgaccac attcgcttat ttgaatctca attgttcccc
    12301 gtctgcgcca gtgacgaaca cggagaagca ctgtttaacc tcgcgcagcc acactttccg
    12361 ttactgaatt acagccgcaa ctcctacatg gggcgattga ttaatcgcac cctgacgcgc
    12421 cacagtaagt tgagtttcag cacctttttt gtctcttcga tgagcgagct tttaaagcag
    12481 gttgccctcg acggctgtgg gattgcctgg ctgccggagt acgccatcca acaagaaatt
    12541 cgcagcggac agctcgttgt actgaatcgg gaagaactgg tgatcccgat tcaggcctat
    12601 gcataccgaa tgaatacccg catgaatccc gttgccgaac gcttctggcg tgaactgcgc
    12661 gagctggaga ttgtgcttag ctgacattct ccagcgtcgc actacgccaa ccgccattat
    12721 cccagaccag atcaaacgtt agcggttcat cgtcgtggta cagcattatc tcaaggctga
    12781 tcgccagcgt gtggcagtac gcttctttca aattgacctg caacgccgca atccgtggct
    12841 catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctgaaga tgtttgtcgc
    12901 tctggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt aatgccgaat
    12961 gaggagaatt aaaaagtgtg aggagatcgc tgcataccga tttacgcagc aatgacgttt
    13021 tattgcctga aatttcaggt aaaacagcct gaagtgattg gcgcatcatt ttgcgcactc
    13081 ctgacgttta gcaacatatg cggcttgccc tgaatgccgg gcaccactgt attgatgctc
    13141 attttccata gccattccct tttgttcatt
//
LOCUS       DARNVM010000135        13161 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.135, whole genome shotgun sequence.
ACCESSION   DARNVM010000135 DARNVM010000000
VERSION     DARNVM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.135"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(7..83)
                     /locus_tag="SI621_003542"
     tRNA            complement(7..83)
                     /locus_tag="SI621_003542"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Met,seq:cat)
     gene            292..1389
                     /gene="mltA"
                     /locus_tag="SI621_003543"
     CDS             292..1389
                     /gene="mltA"
                     /locus_tag="SI621_003543"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEI4066210.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1465..2271
                     /gene="tcdA"
                     /locus_tag="SI621_003544"
     CDS             1465..2271
                     /gene="tcdA"
                     /locus_tag="SI621_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HEI4066211.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2322..2765)
                     /gene="csdE"
                     /locus_tag="SI621_003545"
     CDS             complement(2322..2765)
                     /gene="csdE"
                     /locus_tag="SI621_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HEI4066212.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTMAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAVAKQV"
     gene            complement(2765..3970)
                     /gene="csdA"
                     /locus_tag="SI621_003546"
     CDS             complement(2765..3970)
                     /gene="csdA"
                     /locus_tag="SI621_003546"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HEI4066213.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4162..4389
                     /locus_tag="SI621_003547"
     CDS             4162..4389
                     /locus_tag="SI621_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HEI4066214.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4740..5657
                     /gene="gcvA"
                     /locus_tag="SI621_003548"
     CDS             4740..5657
                     /gene="gcvA"
                     /locus_tag="SI621_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HEI4066215.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5676..6071
                     /locus_tag="SI621_003549"
     CDS             5676..6071
                     /locus_tag="SI621_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEI4066216.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6064..7164
                     /gene="rlmM"
                     /locus_tag="SI621_003550"
     CDS             6064..7164
                     /gene="rlmM"
                     /locus_tag="SI621_003550"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEI4066217.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7208..7939)
                     /gene="fucR"
                     /locus_tag="SI621_003551"
     CDS             complement(7208..7939)
                     /gene="fucR"
                     /locus_tag="SI621_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HEI4066218.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7997..8419)
                     /gene="fucU"
                     /locus_tag="SI621_003552"
     CDS             complement(7997..8419)
                     /gene="fucU"
                     /locus_tag="SI621_003552"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HEI4066219.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8421..9839)
                     /gene="fucK"
                     /locus_tag="SI621_003553"
     CDS             complement(8421..9839)
                     /gene="fucK"
                     /locus_tag="SI621_003553"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HEI4066220.1"
                     /translation="MKQEVILVLDCGATNVRTIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSKLTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9948..11723)
                     /gene="fucI"
                     /locus_tag="SI621_003554"
     CDS             complement(9948..11723)
                     /gene="fucI"
                     /locus_tag="SI621_003554"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEI4066221.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYSHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11756..13072)
                     /gene="fucP"
                     /locus_tag="SI621_003555"
     CDS             complement(11756..13072)
                     /gene="fucP"
                     /locus_tag="SI621_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEI4066222.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3161 a         3280 c         3480 g         3240 t
ORIGIN      
        1 tttaattggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag
       61 ctaccaggct gctccacccc gcgtccgtgg atgcgcacta tactccgctc gcgttttcat
      121 gcaacctttt ttttaccccg atcacggatt cggcatgatt ttgaacaaaa acaaatcact
      181 aaagcctatt ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc attaaacgcg
      241 gcggtttgtt atcttcgttg cgccttattt tttaacctga agaagagaac aatgaaagga
      301 cgttgggtaa agtaccttct tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc
      361 aaaccaaccg atcgcggaca gcaatataaa gacgggaaat ttacccagcc tttctctctg
      421 gtgaaccagc cagatgccgt tggcgcgccg attaacgccg gtgattttgc cgagcaaatt
      481 aaccatatcc gtaattcgtc accgcgtctg tatggcaacc agagtaatgt ttataacgcg
      541 gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt cggcattgat
      601 gcctggcaga tggaaggtgc cgacaactat ggtaacgtgc agtttacagg ctattacacg
      661 ccggtaattc aggcgcgcca tacccgccag ggcgagttcc agtatcctat ttaccgtatg
      721 ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct acgcgggggc attgagcgat
      781 aaatacatcc tcgcttacag caactccctg atggataact tcattatgga tgtgcagggt
      841 agcgggtata ttgactttgg tgatggcagt ccgcttaact ttttcagcta tgcagggaaa
      901 aacggtcatg catatcgcag cattggtaag gtgctgatcg accgtggcga agtgaaaaaa
      961 gaagatatgt cgatgcaggc gattcgtcac tggggcgaaa cacacagtga agccgaggtt
     1021 cgcgagctgt tggaacagaa cccgtctttc gtcttcttta aaccgcaatc ttttgcaccg
     1081 gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca
     1141 attattccgc caggtactac cttgctggca gaagtgccgc tgctggataa taacggcaaa
     1201 tttaacggtc agtacgaatt gcgtctgatg gtggcgctgg atgtcggtgg cgcaatcaaa
     1261 ggccaacact tcgatatcta tcaagggatc gggccggaag ccgggcaccg cgcaggttgg
     1321 tacaaccact atggacgtgt ctgggtgctg aaaacggctc cgggcgcagg taacgtcttt
     1381 agcggctgat gtgatattct gtatgccgtt tttacggata cgacagggtg agggataacc
     1441 tcgccctttt tattctgagg tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt
     1501 tttggtggca cagcgcgtct gtatggtgaa aaagcgttgc aactgtttgc tgacgcgcat
     1561 atttgtgtgg tcggtattgg cggtgtcggt tcctgggcgg cggaagcgct ggcgcgcaca
     1621 gggattggcg caatcacgct tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg
     1681 caaattcatg ccctgcgcga taacgttggg ctggcaaaag cggaagttat ggcggagcgt
     1741 attcgccaga ttaacccgga gtgccgtgta acggtggtgg atgatttcgt gacgccggat
     1801 aacgtagcgc agtatatgag cgtgggttat tcatacgtga ttgatgccat tgatagtgta
     1861 cggcccaaag cggcgctgat tgcttattgt cggcgcaata aaatcccgct ggtcacgact
     1921 ggtggcgcgg gtgggcagat tgacccgacg cagattcagg ttaccgatct ggcgaaaacg
     1981 attcaggacc cgctggcggc gaagttgcgc gagcgcctga aaagcgattt tggcgtagtg
     2041 aaaaacagca aaggtaagct cggcgtggat tgcgtgttct ctaccgaagc gctggtgtac
     2101 ccgcagtcag atggcacggt ctgtgcaatg aaagctacgg cggaagggcc aaagcggatg
     2161 gattgtgcat ctggatttgg tgccgcgacg atggtgaccg ccacctttgg ttttgttgcg
     2221 gtttctcatg cgctgaagaa gatgatggcg aaagcggccc gtcaggggta aacaatcagg
     2281 tcgaatgcga cgcgaacgtc ttatccgacc tggttctgtg cttaaacctg cttcgccaca
     2341 gcgataatcg cctcgcttaa cgcattcaac ccctggcttc gtgaggcgct aagctgcgca
     2401 cgcaatccca gctcatcaaa caatgccagc ggcgactgtg cctgcaattc ggcggcggtt
     2461 ttcccctcaa cggcagtcaa caacaccgcc agcaggccgc gcacaatgcg cccttcgctg
     2521 tcgccaaaga aatgcatctt gccgttttca gccatggtat atcccagcca gacgcggttt
     2581 tcgcatccgg caatctcttt agcctgcgct tttaactcgt ctggcaaagc aggaagctgt
     2641 ttccccagca tgatcaactg gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta
     2701 ttgcgtaacg tttctgcggt tacggttgtg ccgaacggat gtccggcgaa ttgcgggttc
     2761 gtcattaatc caccaataat tccagcgcgc ggtcaacggc attcaccagc gcatccacat
     2821 cactctttgt attatatggc gcaaaagagg cgcgcagtgt gccggttacg cctaattctg
     2881 ccagtagcgg ctgagcgcaa tgctgcccgg cccgcagggc aataccgtac tccgccagca
     2941 gcgtcaccat atcgctgtga tgaacgccag caaaatcaaa ggccagcagg ctggaatcct
     3001 ggcagcgaaa tgagcggaag ccaggacgtt ttgccagcgc atcttccgct agcgttgcta
     3061 agctacggct ccagctttcg gcctggttga tatcgtaatc tgccagccat tccagcgccg
     3121 cgcttaatcc tatgacacca gcgacatttg gcgttccggc ttccagtttc cacggcgcag
     3181 attgagtcgt gaagccgtca aaactcactt cgtgaatcat tttgccgcca cccagccagg
     3241 gtgacatcgc ttccagcagt tctgatttgc catacagcac gccgataccc gtcgggccat
     3301 acagtttgtg accagagaaa gcatagaaat caatatccag ttgctgaaca tccgcaggga
     3361 aatgcaccgc cccctgagca ccatcaacca tcaccaccat cccggctgaa tgagcaaagg
     3421 taatcgctcg cgccagatcc gggcaaccgc cagtcacgtt cgacatctga cccaacgcca
     3481 gaatccgact acggggagta atcagttctg gcaacaaatc gacatccggc agtcgctgcg
     3541 cattaagcgg caatttcacc actttggctc ccgtttgttg ggcgaccatc agccagggga
     3601 cgaggttggc gtggtgttct gccacgctga caataatctc atcgcccggt tgcagacgtg
     3661 gacgcgcata gcattgtgcc accatgttga tggattcagt ggtgccgcgc gtccagacga
     3721 tagttttgtc atctggtgca ttcagtaatt gcgccacttt ctctcgtgca gcttcataac
     3781 gcgcggtcag gcgttgggct tcggcaaact ggctgcgatg gacgtttccg gcgctcaggc
     3841 tgtaaaactg tcgggtggct tcaaccacgg cttcaggttt aagcgcggtc gcggcgctgt
     3901 cgagatagac gcccgcatcc tgaagtgcgg gaaactgggc gcgaaactgc gcgggattaa
     3961 aaacgttcat ggtactcctc ggcttgatga accgatcgtg acgcaaaatt cactttgata
     4021 caaggagagt gctaaccatc agaattatct ggatatcctg gcgaagccgc gtaagtaaca
     4081 tatgctgaat agtgttaggc gttaatttta gcctgttaat caatactatt aagatttatt
     4141 aatcgcttta aggagaagat gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg
     4201 acttttgccc tgagcgcctg ttccggttcg aactatgtga tgcacaccaa tgacggacgt
     4261 accatcgtct ctgacggcaa accacagact gataacgata ccggtatgat ttcgtataaa
     4321 gacgctaatg gcaacaaaca gcagatcaac cgtactgacg tgaaagagat ggtcgaactg
     4381 gatcagtaat tcgcgatcgc aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta
     4441 atcactatgg acagacaggg taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat
     4501 ggtctgaatc gcagaccaat tgcaaacaca acaacacaac atcacaaccg taagccaaaa
     4561 gttcaccaga acacgcattc cgataaaact tttcgttccg gctcaggaag tgccgccact
     4621 ataggtattt gctggtagaa gctcaacgga caatttataa tggctcagat taaaaaaact
     4681 aataggttac acagtgtgat ctaattgtta aattcattta acatcaaagt ttaaaagcca
     4741 tgtctaaacg attaccaccg ctaaatgcct tacgagtttt tgatgccgca gcacgccatt
     4801 taagtttcac tcgcgcagca gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa
     4861 tcaagtctct tgaggatttt ctggggctaa aactgttccg ccgccgtaat cgttcactcc
     4921 tgctgaccga ggaagggcaa agctatttcc tcgatatcaa agagatattt tcgcaattaa
     4981 ccgaagcgac gcgtaaactc caggcccgta gcgccaaggg ggcgttgacg gtcagtttac
     5041 tccccagttt cgccattcat tggttggttc cgcgactttc cagctttaat tcagcttatc
     5101 cgggaattga cgttcgaatc caggcggttg atcgtcagga agataagctg gcggatgatg
     5161 ttgatgtggc gatattttat ggtcggggca actggccggg gctacgggtg gaaaaactgt
     5221 acgccgaata tttattgccg gtgtgttcgc cgctactgct gactggcgaa aaacccttga
     5281 agaccccgga agatctggct aaacatacgt tattacatga tgcgtcgcgc cgtgactggc
     5341 agacatatac ccgacagttg gggttaaatc atatcaacgt tcagcaaggg ccaattttta
     5401 gccatagcgc catggtgctg caagcggcta tccacgggca gggagtggcg ctggcaaata
     5461 acgtgatggc gcaatctgaa attgaggctg gacgtcttgt ttgcccgttt aatgatgtac
     5521 tggtcagtaa aaacgctttt tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa
     5581 tagccgcctt tcgccaatgg atcctggcga aagccgctgc tgaacaagaa aaattccgct
     5641 ttcgttatga acaataattt acgtagggta cgaccatgac cagccgtttt atgctgattt
     5701 tcgccgccat tagcggcttc atttttgtgg ctctgggcgc ttttggcgcg catgtgttaa
     5761 gtaaaaccat gggggccgtt gagatgggct ggatccagac cggtctcgaa tatcaggcgt
     5821 tccatacgct ggcgatctta ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt
     5881 tttactggag tagcgttttc ctcgcgttag gcacggtgct gttcagcggc agcctttatt
     5941 gcctggcgct gtcccatctg cgtttgtggg catttgtcac accggttggc ggcgtgagct
     6001 tcctcgcggg ctgggcgtta atgttagttg gtgctatccg tttaaagcgc aagggcgtaa
     6061 gtcatgaata aggttgtatt gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa
     6121 attaccgata aagccggcca gcgggaaatt ttcggttttg cccgcgtgaa agagaatgcg
     6181 ggttatgtca tttatgaatg ttatcaacct gatgatggcg ataagttaat ccgtgagctg
     6241 ccgttcagtt cattaatttt tgcccgccag tggtttgtgg tgggggaact cctgcagcat
     6301 ttgccgccag aagatcgtat tactcccatt gtcggcatgt tacagggcgt agtagagaag
     6361 ggcggtgaac tgcgtgttga agttgccgat accaacgaaa gcaaagagtt gctgaaattc
     6421 tgccgtaaat tcaccgtgcc gctacgcgct gccttgcgcg atgcgggtgt gctggcgaac
     6481 tatgaaacgc cgaagcgtcc ggttgtgcat gtattcttta ttgcaccagg ctgctgctat
     6541 accggttact catacagcaa caataattcg ccgttctata tgggcattcc gcgcctgaaa
     6601 tttccggcag atgcgccgag tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt
     6661 attcctgcgg atgagtggga tgaacgcctg gcgaacggga tgtgggcggt ggatttgggc
     6721 gcttgccctg gtggctggac ctaccaactg gtgaagcgca acatgtgggt ttattccgtc
     6781 gacaacggcc cgatggcgca aagtctgatg gataccgggc aggtgacgtg gctgcgggaa
     6841 gatggtttca aattccgtcc gacacgcagc aatatttcct ggatggtatg cgatatggtt
     6901 gaaaaaccgg cgaaagttgc ggcattgatg gcgcagtggc tggttaatgg ctggtgccgt
     6961 gaaaccattt tcaacctcaa actgccgatg aaaaaacgct acgaagaagt gtcacacaat
     7021 ctggcgtata ttcaggcaca gcttgatgaa catggcataa atgctcagat tcaggcgcgg
     7081 cagttgtatc acgatcgcga agaagtgacg gtgcacgtcc gccgtatctg ggctgcggtg
     7141 ggtggtcgtc gcgacgagcg ataacaggca aggccggggc gtcgtctccg gcctgctacc
     7201 ctttccgtca ggctgttacc aaagaagttg caacctggcg ctcatcagaa ataatgtgcg
     7261 ttacctcatc cagatgcccg atgcgggctt cccctgaacg attaaattta cttttatcaa
     7321 tcaataacaa cgattgcgcg gcgcgtttta atagcattga tttgtaatca gcgttgatgg
     7381 cattggagtc ccacagtgcg ccgctgctat cgatcccttc acaagaaaaa ataaacagat
     7441 cgatttccag cgatttaagt tgggaaatca gcgagggatt gacgtaacag ccatatttgc
     7501 gctcaagtgt gccgccggaa ctgattagtt gaatgcgttc gcgtttaccg agttcatggc
     7561 aaatcggatg gctattggtg aagacctgga tgttgatgtc aggcaactgg cgtgccagat
     7621 accagcaggt tgaactggcg tctaaggcta ttaccatccc ttcttcaatc cacgcgagcg
     7681 cctcgcgcgc gatatctgct ttatgcgcat aatggctttt cagccggatg tgaaagggat
     7741 cgccactgtc ttgattttga cggtggatat atttagcgcg cccatgattg cgcagaattt
     7801 taccctgcgt ctgtaattca ttgagatcgc gacgaatggt ttctttactg acctttagct
     7861 gttcagagag agcttccgtg gtcaggctgg tatggttcag cagcaggtcg actatcgctt
     7921 gctggcgtgc cgctttcata tttttcaccc catcattcga taaccgcccg cgtcatgcgg
     7981 gcgtaccggt atgagattac ggtgttaccc ctttttttaa aagaatattc ccgtacttcg
     8041 ctcgttcgcc tgtgataacg atcgcaaagg ctttttgtgc ccgttcataa aacgcaaaac
     8101 gattgatgcg gatgatgtca ggacacgggg cttgtagtga aagcgcatta cgataacgtc
     8161 gttctacttc aggatcgaga gtgtcacctt ctaccgccgc catcatcacc agcggcggtg
     8221 cataactgtc cagttcaaat aacgggatta tcgcctggag caagtcgctc accaacaggc
     8281 catcagcgcg gatcacctgc ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa
     8341 taatttcatc tccatgtccc atctctgcca gcactttcaa tagttcggga gaaattaacg
     8401 gcgaaattgt tttcagcatt tcacacttcc tctataaatt caggttcagt ttgcgggtag
     8461 aaataacggt actgataatg aatctgcgcg cgagcttctt ccgggctgtt aaattcccct
     8521 acgccatacc aaccgaacag cgcagctcct gcgacggtcg tttcggcgtc gtcgagaact
     8581 tttaccggaa tatcaagcat attggcttta atctgattcc acaatgtgtt gcgacttcct
     8641 ccacctacta acaataattc agaggcttta aagtgcccga ttttttccag catctgtaga
     8701 ttgcgctgta attgcgcggt taacccttcc agcgccgcgc gatagaaatg cccccgcgtg
     8761 gtattaagcg tcactccttg ccagccagcg ttctgacacg acaataaatc acactgcatt
     8821 ttcacgccat ccgcgccagg cgcgatcaga cgagcctctt caatcaacat ttgccaggga
     8881 gtttcagccg tccagaacag ctttctcacc cattccagca cgccggatgc cagccattgc
     8941 atacctgggt tatacaaccc tgcctgacta tccagttcgc aggtcgaacc ggcgtactga
     9001 cttaacagcg aagtatcaac ctgggcgcta cggaccatta aaatttccca tgtaccggaa
     9061 gagagcacgg gttcattttg ttcggcacca gcgccaaaaa gggcgaactg ggtatcgtga
     9121 cctgcggaaa tcaccggtat gccaacgggt aagccgagca ttgctgcggc gctgttctgt
     9181 aacgtaccaa tctgttcacc cgcttccacc agacgaggga agagtcggcg tggaataccg
     9241 gtggcttgta aaatttgcgg actgaaatcg cgttgctgga tatccagcat ctggctggtt
     9301 ccggccatcg tgatatcagt agtgaattcg ccggttaaac ggtggttaat cagcgacgaa
     9361 ataaacagcc aggcgtgcgc gcgttccagc agttgtggat gattttcttt caaccacacc
     9421 aacttatata acgtattgaa actaaaggct ccgacgccag aaatagcctg caactgctgt
     9481 gcggagatca accgttcaat attgtccatt accgctgctg ttcgcggaca tttccagcta
     9541 atgatcggat agagcagatt gccttgctta tctaccagag cgccatccac accaaaggtg
     9601 gtgacggcga taccgcggat gtggcattca gtcagtttac tattgatttg ccgacagcaa
     9661 tcagcaaagc gttgcaaaat ggcgtctaaa gaccactggt gccaggtgtt gttttccatc
     9721 gcgatatcgc tggcattagg cgttgaggcg cgggcaacaa tttttccctg ccgattaacc
     9781 gcgatggtcc tgacattggt cgcgccacag tcgagtacca ggataacttc ttgtttcata
     9841 atcgctcctg caatatagcc ggataacatt gcttatccgg ctaaccactc ttgcttgttc
     9901 aggcccggta gccggagcga ccgggcatca catcagggag taatgtatta acgcttgtac
     9961 aacgggccgt agttctggca agcgcgataa tcctggcctt caatatccat gccgtgcgcg
    10021 gcccaggcag aaggacgata cactttggtc tcttcaacgt tgtgcataca taccgggata
    10081 cgcagcatgg aggcaagagt gataaagtct gcgccaacgt ggccgatggt tagaaccccg
    10141 tggttagcgc cccagttcgc cattaccgag tacacatccg taaacggccc tttaccggtg
    10201 aggcgcggtg caaaccaggt ggttggccag gttgagttgg tgcgtttgtt gaggatgtca
    10261 tgcacatcct tcggcaattc cacgctccag ccttccgcga tttgcagtac cggtcccagg
    10321 cctttgatga tgttgacacg agtcatggtg aacgggacgc cgccttcggt aaggaagcgg
    10381 gaagagtaac cgccgccacg gaagtattcg tggatcgccg ggcaccattc ggtagcggcg
    10441 aggcaagcgt cagcctcttg ctgagagatt tcccagtgcg gcttcatcgt cgggttacct
    10501 tcgctgtcgc gttgtttaca ggaaccgtcc agcgcagcag aaccggagtt gatcaaatgg
    10561 atgatgccgt gttctgccag tccgtccagt ttatgccccg ttacacgctc aattgcttct
    10621 ggtgaccagt aggtacgcac atcagcaaat acctgagcgg tgccggtgag ctggtgaccc
    10681 attagcattg ccacgccgtt aagactgtcg ttttcggtcg ccacgacaaa gggttcgcgc
    10741 acgccattcc agtcaaatga actgttaagg atcgcttcgg cggtgtcgcc attgggatat
    10801 tgatcggtcc agtgacgttg tccctggaag cccgcagcga tggcgttgta gccaagtgat
    10861 tcttccacgc gaccaatatc ggccagtttg ctgttgcctt gcatcatgtc gcggatacac
    10921 attgccatca gtaaactttc gcgcagaact gcgcggcttt gctcggcatt gcgttgatac
    10981 tgtttgttat tttcatcttc gccatagcgg aagtttttat cagcccaggc cagcgccatt
    11041 tccagttcgg cttcgtcgta aatcttctga tcgatacggc gacgcagttc ggtcatatcc
    11101 accgcctgga ctttcattcc cagccaggat tcaaagaagt tgtgatcaac aatagaaccg
    11161 gcgataccca tcgaaacgcc gcccagcgac agatagcttt tacctttcat gctggcgacg
    11221 gccaaaccgg cgcgggcaaa gcgcagcagt ttttcttcaa catcagcagg aatcgatgtg
    11281 tcatcggcat cctgaacgtc atgactgtaa atggagaatg ccgggatacc tttctggctg
    11341 tgagctgcca gagccgctgc caggtaaaca gcgccgggac gttcagtgcc gttaaagccc
    11401 caaatggctt tcgggcgggt tggatccatg tcgatggttt cactgccata gcaccagcaa
    11461 ggcgttaccg taatggtgag gcctacattc tggctgctga atttttcttc gcaagcagcg
    11521 gcttcagcca tacccgcgat acaggtatcg gaaatgacac actcgacggc agctccgcag
    11581 gcatggcgca gtttctcggt cagcagtgcg gccgtagctt tcgccatatt cattgtttgt
    11641 tcttcaagcg actcacgaac acccatgcga cgaccgtcaa taaccgggcg gataccaatt
    11701 ttcggtaagc tgattttttt cattacagat tcctcacttt attcggaaaa tatgttcagt
    11761 tagttgccgt ttgagaacgg aaacgggcaa agataaagat gactgcgaag cagagtgccg
    11821 ggatcagttc ggcagtgggg atgttgcccg ctgcatcact gacaaacccc atgaccggag
    11881 tgacaatacc gccgccaata atggtcataa cgatgaagga cgaaccgtat ttggtgtcct
    11941 ggccgagatt tttaatgccc agcgagaaga ttgttgggta ctgaatcgac ataaaggcac
    12001 tgcataaagt cagggctatt aagcccacat gaccgccagc gaaggctgag atcaggcaca
    12061 gtgccatagc gattaatgcg taggctgcca ggactttgtg tggtgcgaag cgactgatga
    12121 gccaggtacc ggtgaaacga ccaataaaga agcacaccat ggttccggtt aaatagttag
    12181 cggcaaagcc tgcggtcata cctggaattt cttctacagc gtagcgaatc aaatagctcc
    12241 agcaggccgt ttgcgcgccg acatagcaga attgcgctaa taccgcccag cgccagtgac
    12301 gaatacgcgc cagacgagaa agcgatgcgg agaacgatcc ttgtttggcg tcactgtgat
    12361 tatcactctg caatgccggg aatttcgtca gcatgatcag cagggcgacc agtaacacga
    12421 tagccacgat gatcatataa ggtgtctgta ccgataatac caggctgtgt ttatacgcac
    12481 tcaattgctc tggagacatt ttatcgagaa cgtcttgcga ttgatgtggc acgttagaca
    12541 aaataagact ttgtccaaag acaaccgcga taattgcgcc aaacgagtta aatgtttgcg
    12601 caagatttaa gcggaagtga ccactgcttt ccggccctaa taccgtaaca aaagggtttg
    12661 cggcagtttc cagacaacct aatccggctg caataataaa taggccaact aaaaataagg
    12721 tgtagttcat tatttctgca gcgggccaga ataatgcagc gcccaaggca tataaaaata
    12781 atccggtaat aatccctgct ttataactga gttttttcat caatatccca gcagggattg
    12841 ggataatgaa ataaccaaag taaaaggccg attggatcag gccagcctgg aaatttgtca
    12901 gcgtaaaagc ctgctggaat tgaggtaata aaatgtcgtt aaggttattg gctaccgccc
    12961 aaagaaaaaa cagtgagcac agcagcgcga atggaataat gtaacttctg ctttgccctg
    13021 catctttatc taccgcacgg taactctgcg tttgtattga tgtgtttccc atagcatcct
    13081 cttaggttca gaagcttaag cacgtaatcg gttatgtttt caaaagtgca aaaccagcga
    13141 gttcgggctt catatcagtg a
//
LOCUS       DARNVM010000136        13119 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.136, whole genome shotgun sequence.
ACCESSION   DARNVM010000136 DARNVM010000000
VERSION     DARNVM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.136"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..836
                     /locus_tag="SI621_003556"
     CDS             <1..836
                     /locus_tag="SI621_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4066223.1"
                     /translation="LKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKI
                     LFDNCILDDSNFAQIKMTDGTLNSCSAIHVQFYNAAMNRANIKNTFLDYSNFYMAYMV
                     EVNFYKVIAPYVNLFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTR
                     LTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKAWMFNTRLYRVNFDEANVQG
                     MGISILREEENIPINSDTLITLQKFFEEDCTSHTGMSQTEDNIHAVAMKITADIMRDA
                     D"
     gene            complement(990..2639)
                     /locus_tag="SI621_003557"
     CDS             complement(990..2639)
                     /locus_tag="SI621_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEI4066224.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2790..4139)
                     /gene="ghxP"
                     /locus_tag="SI621_003558"
                     /gene_synonym="yjcD"
     CDS             complement(2790..4139)
                     /gene="ghxP"
                     /locus_tag="SI621_003558"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HEI4066225.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4685..5149)
                     /gene="soxR"
                     /locus_tag="SI621_003559"
     CDS             complement(4685..5149)
                     /gene="soxR"
                     /locus_tag="SI621_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEI4066226.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5235..5558
                     /gene="soxS"
                     /locus_tag="SI621_003560"
     CDS             5235..5558
                     /gene="soxS"
                     /locus_tag="SI621_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HEI4066227.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5561..7147)
                     /gene="pdeC"
                     /locus_tag="SI621_003561"
     CDS             complement(5561..7147)
                     /gene="pdeC"
                     /locus_tag="SI621_003561"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HEI4066228.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVVVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7577..7858
                     /locus_tag="SI621_003562"
     CDS             7577..7858
                     /locus_tag="SI621_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HEI4066229.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7957..8493)
                     /gene="ssb1"
                     /locus_tag="SI621_003563"
     CDS             complement(7957..8493)
                     /gene="ssb1"
                     /locus_tag="SI621_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HEI4066230.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8748..11570
                     /gene="uvrA"
                     /locus_tag="SI621_003564"
     CDS             8748..11570
                     /gene="uvrA"
                     /locus_tag="SI621_003564"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEI4066231.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11605..11961)
                     /locus_tag="SI621_003565"
     CDS             complement(11605..11961)
                     /locus_tag="SI621_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEI4066232.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11965..12381)
                     /locus_tag="SI621_003566"
     CDS             complement(11965..12381)
                     /locus_tag="SI621_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEI4066233.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12492..>13119)
                     /gene="aphA"
                     /locus_tag="SI621_003567"
     CDS             complement(12492..>13119)
                     /gene="aphA"
                     /locus_tag="SI621_003567"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEI4066234.1"
                     /translation="PLNPGTNVARLAEQAPIHWVSVAQIENSLAGRPPMAVGFDIDDT
                     VLFSSPGFWRGKKTFSPESEDYLKNPVFWEKMNNGWDEFSIPKEVARQLIDMHVRRGD
                     AIFFVTGRSPTKTETVSKTLADNFHIPATNMNPVIFAGDKPGQNTKSQWLQDKNIRIF
                     YGDSDNDITAARDVGARGIRILRASNSTYKPLPQAGAFGEEVIVNSEY"
BASE COUNT         3418 a         3430 c         3177 g         3094 t
ORIGIN      
        1 gcctgaaagg tatcgtgatt aaagatgaag atttatccaa ttcaaatttt gcaggttgca
       61 gactacaaaa cgctattttc caggactgta atatgtataa aacgaatttt aatttcgcca
      121 taatggaaaa aatacttttt gataattgta ttctcgatga ctcaaatttc gctcagataa
      181 aaatgactga cggaactcta aattcatgct ccgctatcca tgttcaattc tacaatgcag
      241 caatgaatag agccaatatt aaaaatacct tccttgacta ttcaaatttt tatatggcgt
      301 acatggttga ggtaaatttt tataaagtaa tagcgccgta tgttaattta tttagagccg
      361 atcttagttt ctctaaactc gatttaatta actttgaaca tgctgatctg tctcgcgtca
      421 atctgaacaa agcaactctc cagaatataa acttaattga tagcaaactc ttttttacgc
      481 ggctgacaaa tacattcctt gaaatggtta tatgtaccgg ctctaatatg gctaatgtta
      541 attttaataa tgccaattta agcaattgcc atttcaactg ttctatttta acaaaagcct
      601 ggatgtttaa tacccgtctc tatcgggtta atttcgatga ggctaacgtg cagggaatgg
      661 gcatttccat tctccgtgag gaggaaaata tccccattaa tagtgatacc ctgataacac
      721 tacagaaatt ctttgaagaa gattgtacct ctcatactgg catgtcacaa actgaggata
      781 atattcatgc agtcgctatg aagattactg cagatattat gcgagatgca gattgaggtt
      841 aagccgaaga acagccaatg aattgacttt aaaagagaga taacttattt gttttagaat
      901 catgtatgtt agcccgagct aacctgttaa aggtgcaaaa gattgcacct ttttcaaatt
      961 acctcgggcc tggttacgtc gctttcggct tactgatttt cctcaatcaa caacgcttca
     1021 agcaaatcga gatcgtgcag taatttttgc agcgtttcat tgctgatctc ccgcgtggcg
     1081 cgcaggtggt acagttcagc acgttcagaa cgcaatgccg ccagacggaa gcgacgctca
     1141 aggttctctt cctgcacgga actttcaacg tcgttacgtc catcggcgcg acgacgcagg
     1201 ttaccaatga cgcgagaact gacctccgtg agcagctggt tatcgatatt ctcttcggta
     1261 tccgccgcca gacgctcctc cattttctgg atcgccacaa tcgccacttc tgccgttgcc
     1321 gctcgcgcaa tacgttcctc tttcaattgt tgcgaatggt ccgcgacttc aatgtgttgt
     1381 agcagaatag gcaacatcac cacgccgaca aacagcgaga agagaatgac accagccgcc
     1441 aggaatacca gctcatagcg cgccgggaag acgttaccat ccggcaagag cagcgggata
     1501 gagagcacac cagccagagt gatcgcccca cgcaccccgg cgaaagacgc gatcaggatt
     1561 tctcgtgtgg tccacgaacc aaactccatc ggcttctttt tcaggaagcg gttgctgaac
     1621 tttttcatcg tccacagcca gccgaaacgg accagcatca gcgccgcata aatcagaatg
     1681 atatcggtaa acagcatcca gatttcgacg ttagggtcga tttctgccgc catcagcgac
     1741 gtctccagaa tacccggcag ctgcagacct aacagcagga ataccatgcc gttaaagaca
     1801 aattccagca tcgcccaggt gctgtttgcg cgcaggcgca ttgccagcgg cgcacggcgc
     1861 atcacaccgg agcgggtgat ggtcatcccg gcagcaaccg cagcgaggat gccggaaacg
     1921 ccaatatgtt cggcaatcag ataagaagcg aatggcagca ggaacagcag gacgatctgc
     1981 gtcgcgggtt catcaccgcc ccagcggctg aggaatcgca gcgagcgacc gtacagccag
     2041 ctcaccacaa aaccggcgag aataccgcca atggctactt tcataaattc gaccgtcgca
     2101 ccgccaacgg tgaagatcat cgtccccatc gccactgcta cggcaaactt caacgacacc
     2161 aggccagagg cgtcattcat caacgcttcg ccctgcaaaa tgcccatgat ttttttcggg
     2221 atgcgccctt cgccaacaat cccggagagc gccacagcat cggtcggaga aagcaccgcc
     2281 gccagcgcaa aggcggggat cagcggaatg cccggcacca cccagtaaat aaggaagcca
     2341 atgccgacca cggtgaccac caccagcgcc agcgcgaggc cgaaaatctc tcgaccatgt
     2401 tcgagaaatt cacgggtcgg cgttttccag ccatcagcga acagcaacgg cgggataaac
     2461 aagactaaaa agagttcagg atcaaactcc acatgcaaac caaacgtcgg ccacgccagt
     2521 agcgcaccga tggcgatttg cataagcgga agcgggatct gaaagggcat gacacgagtg
     2581 accaccccgg acagcgagac cacgagggtc attatcagta tggtgaagaa gatttccatg
     2641 cgttccctgt ttgcgatgtt ttttatgatc tgagaaagac gttatgcctg aattctcatt
     2701 ttttcagaat atcgcaaaag ctcacacaat gtgcggagaa aataaaaacg ggcagccatt
     2761 gccacccgtt ttcagaatca tatgaaagat tagatagccc agccacccgc atagaaggtc
     2821 accagcgcca cagcgataac gaccgtaccg atgttcaact tgcgccattc gccggaaacc
     2881 agacgaccaa tcaccagagt cgcgaagccg atcatgatgc ctgttacgat gttacaggtc
     2941 agcacgatga ataccgccgt aaccagaccc gccatcgcat caacaaaatc agcaaagtcg
     3001 attttcgcca cgttactcag catcagcagg ccaacgtaca tcagcgccgg agccgttgca
     3061 taccccggaa cgaggtaaga gagcggagag aggaacagaa tcagcaggaa cagcacgcca
     3121 acggtgatag ccgtcaaccc ggttttaccg cccgccgccg tacccgccgc agactcgatg
     3181 tataccgctg ccggagctgc acccaccagg ccagagaaaa cgctgctcat ggagtcggtg
     3241 gtcagtgctt tcccaccgtc aatgatctgc ccatctttat ccagcaggtt cgcctgaccg
     3301 gcgacggcgc ggatagttcc ggtggcatca aatactgccg tcatcaccag cgccagaacg
     3361 cttggcagga ctacaggatt cagcgcgccc ataatgtcca ggctaccaat cagtgaattg
     3421 ccgttttcat cgctcaatga aggcatggcg aaaacgccgg agaaatggac gttaggatcg
     3481 aagatcaaac cgacaattga gataccgata atggtcagca gaatgccacc agggactttc
     3541 agtttttcca ggccgatgat caccgccaga cctaccagtg acataatcac cgggaaggtc
     3601 gcgaaatcac ccagcgcaac gggcagacca tcaagcgggt ttttaatcac cagaccgaca
     3661 ccattagcgg caatgagcag caggaacaga ccgataccga tccccgtgcc gtgcgccaca
     3721 ccgtgcggca agttgcgcaa aatccagcta cggatacccg tggcagaaat taccgtaaac
     3781 agtacaccca tcaggaacac agcacccagc gcgacaggta cgctaatatg ttgccccagc
     3841 accagactga atgcggtaaa cgccgtcagg gagatggcgc aaccaatcgc caacggcaga
     3901 ttagcccaca gacccatcac gatagaaccg agtccggcaa ccagacaggt cgcaacgaaa
     3961 actgccgcag gcgggaagcc tgctttaccc aacatgcctg gaacgacgat aaccgagtag
     4021 accatcgcca gaaatgttgt taacccggca accacttcct gacggacagt gcttccacgt
     4081 tgtgaaattt taaaccaggc gtcgagtgaa ccgccggtac gcgctgatgg cgtagacata
     4141 agaaaacatc ccctgagagt ttaattttcg tcaagatgcg tggcggacaa tcccctgcca
     4201 gcgaaacgtt atcttcctgc tccaggtttg cgacaaattg gtgcgtcgca aaaaagcaaa
     4261 cgtttagctc cgcgcataca aaacgggtgg taaaaaaagc aaacgattat ccggcgatca
     4321 tagcggcttt ttcaaccgaa gtttgctgct tttttctctt ttattgtctt gacgatgaaa
     4381 aaccaggcaa ttgatgcgca aacgttatcg tcgatgcgca agattgcagc gttatcgagc
     4441 catataccgt tcaggattgc gataaaaaat cgtcttagat gacaaaaata ttgcggctaa
     4501 aaagtgtact tttactctgg cgaagaaacg ccggtgacat acaattaaag catcaacacc
     4561 aaccggaacc tccaccacgt gctcgaatga ggtgtgttga cgtcggggga aaccctcctg
     4621 tgtaccagcg ggatagagag aaagacaaag accggaaaac aaactaaagc gcccttgtgg
     4681 cgctttagtt ttgttcatct tccagcaagc gtgcgcctgt gccttcttct cctaagcggt
     4741 cgcccgggtt acgcaatggg caatcactgc gcgaaaggca gccacaacca atacatccgt
     4801 ccagctcgtc acgcagcgcc actaaggtat gaatgcgccg atccaactct tctcgccatt
     4861 gggacgaaag ctgtttccac tctttcgcac ttaatgtatg cccttcgggc aacacgccga
     4921 atgcttcacg aatggtcgcc agcggaatgc caatacgctg agcaattttg atgattgcga
     4981 catatcgcaa cacatcacgt ttatatcgcc gctgattgcc actattacgg atactgctaa
     5041 tcaacccttt gctttcatag aaatgcagcg ccgataccgc cacaccgctg cgttttgcca
     5101 cttcgccggg ggttagcagc gctttaatgc ggggtaattt cttttccata aatcgcttta
     5161 cctcaagtta acttgaggaa ttatactccc caacagatga attaacgaac tgaacactga
     5221 aaagaggcag atttatgtcc catcagaaaa ttattcagga tcttatcgca tggattgacg
     5281 agcatattga ccagccgctt aacattgacg tcgtcgcaaa aaaatcaggc tattcaaagt
     5341 ggtacttaca gcggatgttc cgcacggtga cgcatcagac acttggtgat tacattcgcc
     5401 aacgccgcct gttactggcc gccgttgagt tgcgcaccac cgagcgtccg atttttgata
     5461 tcgcaatgga cctgggttat gtctctcagc agaccttctc ccgcgttttc cgtcggcagt
     5521 ttgatcgcac tcccagcgat tatcgccacc gcctgtagtt ttattgcccg cgcgttaact
     5581 cccgcgcggg taattgctcc atccattgca taaacacctg cggcggcatc gccttcgcaa
     5641 agaaccatcc ctgacaatag cgcacgccgc gtttgcgcag ccagttaacc tgctcctcag
     5701 tttcgacgcc ttcagcgatc gtttttaacc ccagactgtg cgccagctcg atgatgtgtt
     5761 ccgcaatcaa atgactggtt ttgtgggtgg tcagcgtctc aacaaacgat ttatcgattt
     5821 tcaaaatatc gacattcaat gatttaaggt tatgcaagtt agagtagcca ataccaaaat
     5881 catcaattgc cacttcgtaa ccagcctggc ggaacgccag aataattggt gtcattttgt
     5941 cgacatcaag aaatgcatgt tcagtcactt caaatttaat ttgctgtgga cgcaccgcgt
     6001 attgctctgt tttctgattg gttcgcgcta tcaaccgtga cgtatgaaaa tcggaggccg
     6061 acaggttaat agaaacatag cgatcggcat gtgttgccag gtacgcgccc agatcgcgga
     6121 agacattatc aataacataa tcagttacct gtccgatcat cccctccttt tctgccagcg
     6181 gaataaactc tgccggattc attacttgcc cctgctcacc aggccaacgt aacaatgctt
     6241 cagcgccgat acatttttct gttttgatat cgattattgg ctggtaataa aggcaaagtt
     6301 gatgtttttc gagggcgcgt tgcaatttgc gtttgggaga caaatagttt tgccgaatac
     6361 gtagccagag taataacaga acaagactcc ccaaaatacc ggcgggtaac gcaaaaataa
     6421 gatgattata aaaatgcgct ataagacgtt gatatgaagt cgcaacaatg gctgcaattg
     6481 ggcgttttgt cgaataaact gtcgcatata aatagccatt tctttgtacg gttaaatcat
     6541 tcaaatgaat cagtggagaa aacgttgctg ccgaggcctc gttgcttaac gagaaaaagg
     6601 ttttcgttac cgtatcatac acaccccatt gcaatgtcgg atcatcagac atcacttcac
     6661 tccagaagag agggttgatg accaccacat aatttccccg ctgcatatag gtcattttat
     6721 agccagagaa aaaaggcgta tcgcggtaat aatagataga aacgttaggt tcacgcttat
     6781 aatcggccgg tgcaatcgta tagccgttta caggcgctat cagcgatgag cataaaaaat
     6841 ggttatcacg ggcatagatc aattcattaa tataaagata gccacgaata atattcaaca
     6901 ttcgcttttg atgggctgga gtgcaaactt gcccctgata gcgttctgct gcatcgctca
     6961 ctaaatctgc ctggcgaatg accagctcgg atttatccag cgccagctga gcaaaagttt
     7021 ggagctgact attcacttct gattttgccc aaaggaaggc aatccatagc gacagtatga
     7081 tgggaaagag aactaaaaag ataatgcccg gcaacgccag taattggtgt cgtgcacgat
     7141 gactcattgc tatcaatttc ctgcctgttt atacccggtc attgcaaggc gagaaccctg
     7201 gttatcgacg tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat
     7261 tgataccttt ttaatcgtca cctaaataaa aataattcat tgatattcaa aactaatttc
     7321 ctacaggaat gcagaggcgg tgggaagata tccccttaat gccgctgaaa aaacacataa
     7381 tctgtacggc aataaaagtc gcggcctaat ctggctaaca aacaatcaat tgtgatatag
     7441 ttcacaaaat taatgaaaca aacagagtgt ttcttttttg tgttctgcaa gccccgcgat
     7501 agccagattg tatcacgcct cccgtgaaca acgccgcgct gtccgaggca tcggctcttt
     7561 tcagaggaat tgtttgatgg ctaccctcac cactggcgtg gttcttcttc gctggcaact
     7621 tcttagtgcc gtaatgatgt ttctggccag cacactcaac atccgttttc gtcggtctga
     7681 ttatgtcggg cttgcagtga tcagcagcgg tctgggcgta gtttccgcct gctggttcgc
     7741 aatggggttg cttggcatca caatggcgga tatcaccgcc atctggcaca acatcgagtc
     7801 ggtgatgata gaagagatga atcagacacc gccacaatgg ccaatgattt tgacttgata
     7861 tgtagaagcc tccaaaacgg aggcttcttt tttacggctg gcagatgttt taatcatcca
     7921 ccttaaaaca atataaccta ttgttttaat gaaaaatcag aacggaatat cgtcatcaaa
     7981 gtccatcggc ggctcattag acggtgctgc cggagcagac tgctgcgggc gagactgcgc
     8041 gccgccgctg aactgattgc cgccctgcgg ctgctgaggc tgaccccaac cgccctgcgg
     8101 ctggccacca ccgatattgc cacctgcagg agcgccacca ccctgacgac cacccagcat
     8161 ctgcatggtg ccgccaacgt tcaccaccac ttcagtggtg tagcgatcct gaccggattg
     8221 atcggtccat ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag
     8281 atattcgctg gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg
     8341 ctctttcatc tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt
     8401 ggcaacagcg ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac
     8461 gagaataacc ttgtttacgc ctctgctggc catgttcgtg tctcctgaaa aaaaatcgtt
     8521 ctgaataagt gtaaacgcgc gattgtacca ttaccaatag cgcttttact atgttgtgac
     8581 ctcggttccg ggaaacaaac ctggccagac attgttacac aacactccgg gtaatgcatt
     8641 ccaatactgt atattcattc aggtcaattt gtgtcataat taaccgtttg tgatcgccgg
     8701 tagcaccatg ccaccgggca aaaaagcgtt taatccggga aaggtgaatg gataagatcg
     8761 aagttcgggg cgcccgcacc cataatctca aaaacatcaa cctcgttatc ccccgcgaca
     8821 aacttattgt cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct
     8881 tatatgccga agggcagcgc cgttacgttg aatccctttc cgcctacgcg cggcagtttc
     8941 tgtcactgat ggaaaagccg gacgtcgatc atattgaggg gctttctcct gccatctcaa
     9001 ttgagcagaa atcgacgtct cataacccgc gttctacggt ggggacaatc accgaaatcc
     9061 acgactattt gcgtttgttg ttcgcccgcg tcggtgagcc gcgctgcccg gaccacgatg
     9121 taccgctggc ggcgcaaacc gtcagtcaga tggtggataa cgtgttgtcc cagccggaag
     9181 gtaaacgcct gatgctgctc gcaccaatca ttaaagagcg caaaggcgaa cacaccaaaa
     9241 cgctggaaaa tctggcaagc cagggttaca tccgtgctcg tattgatggc gaagtctgcg
     9301 atctttccga tccgccgaaa ctggaactgc aaaagaaaca taccattgaa gtggtggttg
     9361 atcgcttcaa ggtgcgtgac gatctcaccc aacgtctggc agagtcgttt gaaaccgcgc
     9421 tggagctttc cggtggtacc gcggtagttg ccgatatgga cgatccaaaa gcggaagagc
     9481 tgctattttc cgccaacttc gcctgcccaa tttgcggcta tagtatgcgt gaactggaac
     9541 cacgcctgtt ttcgtttaac aacccggcgg gggcctgccc aacctgtgac ggccttggcg
     9601 tgcagcaata tttcgatcct gaccgcgtga tccagaatcc ggaactgtcg ctggctggcg
     9661 gtgcgatccg aggctgggat cgccgcaact tctattactt ccagatgctg aaatcgctgg
     9721 cagatcacta taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata
     9781 aagtggtgtt gtacggttct ggcaaagaaa acattgaatt caaatacatg aacgatcgtg
     9841 gcgatacctc cattcgtcgt catccgttcg aaggcgtgct gcacaatatg gagcgccgtt
     9901 ataaagagac ggaatccagt gcggtacgtg aagaattagc caagtttatc agcaatcgcc
     9961 cgtgcgccag ctgcgaagga acccgtctgc gtcgggaagc gcgccatgtg tatgtcgaga
    10021 atacgccgct gcctgctatc tccgacatga gcatcggtca tgcgatggaa ttcttcaaca
    10081 atctcaaact cgcaggtcag cgagcgaaga ttgcggaaaa aattcttaaa gagattggcg
    10141 atcgcctgaa attcctcgtt aacgtcggcc tgaattacct gacactttcc cgctcggcag
    10201 agacactttc tggcggtgaa gcccagcgta tccgtctggc gagccagatt ggtgcgggcc
    10261 tggttggcgt tatgtacgtg ctcgatgagc cgtctatcgg cctgcaccag cgcgataacg
    10321 agcgcctgtt gggtacgctt atccatctgc gcgatctcgg taataccgtg attgtggtgg
    10381 agcacgacga agacgcgatt cgcgccgctg accatgtgat cgacattggt cctggtgcgg
    10441 gtgttcacgg cggtgaagtg gtcgcggaag gtccactgga agcgattatg gcggtgccgg
    10501 agtcgttgac cgggcagtac atgagcggta aacgcaagat tgaagtgccg aagaaacgcg
    10561 ttccggcgaa tccggaaaaa gtgctgaagc tgacaggcgc acgcggtaat aacctgaaag
    10621 acgtgacgct gacgctgcca gtcggtctgt ttacctgcat cacaggggtt tcaggttccg
    10681 gtaaatcgac actgattaac gacacactgt tcccgattgc ccaacgccag ttgaatgggg
    10741 cgaccatcgc cgaaccggca ccgtatcgcg atattcaggg actggagcat ttcgataaag
    10801 tgatcgatat cgaccaaagc ccaattggtc gtactccgcg ttctaacccg gcgacctata
    10861 ccggcgtgtt tacacctgtg cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgcg
    10921 gctatacgcc gggacgtttc agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg
    10981 gcgatggcgt gatcaaagtg gagatgcact tcctgccgga tatctacgtg ccgtgcgacc
    11041 agtgcaaagg taaacgctat aaccgcgaaa cgctggaaat taagtacaaa ggcaaaacca
    11101 tccacgaagt gctggatatg accatcgaag aggcgcgtga gttctttgat gcggtgccag
    11161 ctctggcgcg taagctgcaa acgttgatgg acgttggcct gacgtacatt cgcctcgggc
    11221 agtccgcaac cacgctttct ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt
    11281 caaaacgcgg caccgggcag acgctgtata ttcttgatga gccgaccacc ggtctgcact
    11341 tcgccgatat tcagcaactg ctcgacgtac tgcataaact gcgcgatcag ggcaacacca
    11401 ttgtggtgat tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg
    11461 gaccagaagg cggcagtggc ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg
    11521 cggagtgcga agcttcgcat acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc
    11581 cgctttccga gcggcctttt cctttcagag ttgcaccagc aatttacgtt tttcttccgg
    11641 cagcaaattc accgcctgct gataagacgc atccaccaga taatagattt gcgaatctgg
    11701 cagcgaaccg tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggcttggacg
    11761 cacatcgctg tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac
    11821 cgccgggcga ttttctactt ctttcaccat cgcaaacagt acatcttcca ctttgatctg
    11881 cgtcgctttc cagtcgttat gcacgctctg ttctgcgcct ggttttgcca tgcaatattg
    11941 tagcaactcc gaaatggtca ttttttactc cccttgtagt gtcgcgataa tgcgacgcga
    12001 tccgccgtgg atgcgatgtt cccccagcca gatgccttgc caggtgccgg tctgaatacg
    12061 ccctttatgc accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg
    12121 catatcgtct gctccctcat agtcatgctc ataatttccg ttgtcgggaa cggtgcggag
    12181 gaaaaaacgc tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga
    12241 ggcggaggta tgttgcagca acagatgcag taaaccgatg ttaacgcgcg gcatatcagc
    12301 cagctgattc agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt
    12361 gagcgtcttt tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag
    12421 caagacagag aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gtgtcacgct
    12481 ctcccgctct gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt
    12541 ggcaagggtt tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca
    12601 tcgcgtgcgg cggtaatatc gttatcggaa tcgccataaa aaattcggat atttttatcc
    12661 tgcagccatt gcgattttgt attttgccct ggtttatcac ccgcaaagat caccggattc
    12721 atattggtgg caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc
    12781 gtcgggctac gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc
    12841 agctggcgag cgacctcttt tggaatgctg aattcatccc agccattgtt cattttttcc
    12901 cagaacacag gatttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc
    12961 cagaagcccg gactggaaaa aagtaccgtg tcatcgatat caaaccccac cgccattggc
    13021 ggacgccctg cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt
    13081 tcagcaagcc tggcaacgtt agtaccaggg ttaagcggc
//
LOCUS       DARNVM010000137        12947 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.137, whole genome shotgun sequence.
ACCESSION   DARNVM010000137 DARNVM010000000
VERSION     DARNVM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.137"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..313)
                     /gene="yafO"
                     /locus_tag="SI621_003568"
     CDS             complement(<1..313)
                     /gene="yafO"
                     /locus_tag="SI621_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HEI4066235.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     IL"
     gene            complement(316..609)
                     /gene="yafN"
                     /locus_tag="SI621_003569"
     CDS             complement(316..609)
                     /gene="yafN"
                     /locus_tag="SI621_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HEI4066236.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(661..1716)
                     /gene="dinB"
                     /locus_tag="SI621_003570"
     CDS             complement(661..1716)
                     /gene="dinB"
                     /locus_tag="SI621_003570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEI4066237.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1787..2572)
                     /gene="lafU"
                     /locus_tag="SI621_003571"
     CDS             complement(1787..2572)
                     /gene="lafU"
                     /locus_tag="SI621_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HEI4066238.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISLSH
                     PPKPATVAVPEETEKKARDVNEKMALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2544..4256
                     /locus_tag="SI621_003572"
                     /pseudo
     CDS             2544..4256
                     /locus_tag="SI621_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            4361..4639
                     /locus_tag="SI621_003573"
     CDS             4361..4639
                     /locus_tag="SI621_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEI4066239.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            4632..4988
                     /locus_tag="SI621_003574"
     CDS             4632..4988
                     /locus_tag="SI621_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEI4066240.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(5045..5794)
                     /locus_tag="SI621_003575"
     CDS             complement(5045..5794)
                     /locus_tag="SI621_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEI4066241.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6095..6835
                     /locus_tag="SI621_003576"
     CDS             6095..6835
                     /locus_tag="SI621_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEI4066242.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6806..7573)
                     /locus_tag="SI621_003577"
     CDS             complement(6806..7573)
                     /locus_tag="SI621_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEI4066243.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7678..8256)
                     /gene="lpcA"
                     /locus_tag="SI621_003578"
     CDS             complement(7678..8256)
                     /gene="lpcA"
                     /locus_tag="SI621_003578"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEI4066244.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8496..10940
                     /gene="fadE"
                     /locus_tag="SI621_003579"
     CDS             8496..10940
                     /gene="fadE"
                     /locus_tag="SI621_003579"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HEI4066245.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPDKVRKVEAA"
     gene            complement(10983..11429)
                     /gene="ivy"
                     /locus_tag="SI621_003580"
     CDS             complement(10983..11429)
                     /gene="ivy"
                     /locus_tag="SI621_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HEI4066246.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11610..12380
                     /gene="yafV"
                     /locus_tag="SI621_003581"
     CDS             11610..12380
                     /gene="yafV"
                     /locus_tag="SI621_003581"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HEI4066247.1"
                     /translation="MSGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            complement(12475..12786)
                     /locus_tag="SI621_003582"
                     /pseudo
     CDS             complement(12475..12786)
                     /locus_tag="SI621_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12805..>12947)
                     /locus_tag="SI621_003583"
     CDS             complement(12805..>12947)
                     /locus_tag="SI621_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEI4066248.1"
                     /translation="FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
BASE COUNT         3039 a         3342 c         3426 g         3140 t
ORIGIN      
        1 tcagaatggc aatgagcaac catgcttgct catcaaacgc accctgacaa tataccaaat
       61 gcgcttcgtc attcgttctg ctgaattggc gcaactgtgg cggaaatgga ttattcacat
      121 ttgccagatg aatatgagca actcgctcaa atttgattaa tggccaggta aacgagtcgt
      181 cgtagagtgc atcgcgacca aatatatctg gcaaaacacc gtcacgctta taggaaataa
      241 aatccgccgt taacgcatca agttcctctg ctgtaagttg caggcgaata agttttgttt
      301 tgaatacccg catccttatt ccttaaagtc attgaaatca tcatccgtca tatcattaag
      361 atattgttca gcgcggcgtg taatttcctc taatcttgcg gcactcggac ggaagcgcgc
      421 cttcaccagt tttttctcct cttgttctgc aagcatgtcc attaacgctt cgaatgcgct
      481 ggcacttaag agatatcctg cggggcgatt attagaaaga accgcaaccg gttgatcaat
      541 aaagtatttt gctggatttt tacgtaactc agtgatattg accgattttt cagcgagaat
      601 tcgatgcata cagtgatacc ctcataataa tgcacaccag aatatacata atagtataca
      661 tcataatccc agcaccagtt gtctttccat ttgcgggtca agcaacgtca catgcagccc
      721 caccagccgc acaccgcgcc cgccgcgacg ttcatcccag gttttacgcg cggtggcaat
      781 taagtcagct ttattcagcc gcggccagac gtgctcctga gtggtttgct gaaaatcatc
      841 aaactttaat ttcaccccct ggcgagcaat cagtaaatca ggttttactt ttgccagacg
      901 gcgttcaagt tccgggtaca gccgctcgat aatcgcttca cattcagacc agtgatgaat
      961 atcttccgcc atcgtgcgtt ccacgccgac ggattttcgc aaccgttcgc tgttaacgtc
     1021 gcgctcgtca atcccctgac tacgctccca caaaatgcgg ccaaatttgc caaaacgttt
     1081 gagcagagtc accagatcac acttttgcac atcaccgcag gtccgcagcc ccatcgcttc
     1141 cagttttgcc gccgagactt tgccgacgcc ggggattttt gccagcggta aggtttgtaa
     1201 aaatgtcgga acttctgccg gcgtaatcac aaactggccg ttgggtttat tcatgtcgga
     1261 ggcgattttg gcgagaaact ttactggtgc cacgcccgca gacgccgtca gttgcagctc
     1321 gctgaaaatc gtctggcgga tttcctgggc gatgagggtc gcagaaccgt ggcaatggac
     1381 gctatcggtg acatcgagat aggcctcatc cagtgacaac ggttcaatgc gcgaggtgta
     1441 gcgcgagaag atttcacgga tatgatttga ggcttctttg taggcgtcaa agcgccccgg
     1501 aagcaaggtg agatgtgggc ataatttgag cgccatccca gtaggcatag cgctacgtac
     1561 gccaaattta cgcgcgggat aattggcggt gctgatcacc ccccgacgtt cgcggctgcc
     1621 gccaatagca atagggatat cgcgcagggc gggattgtcg cgcatctcca ctgcggcgaa
     1681 aaagcagtcc atatccacat gaatgatttt acgcattgct cacctctcaa cactggtaaa
     1741 gtatacagtg atttcagggt ttgagaaatg cgtaaagatt cagcatctag cgcgttcgct
     1801 gcgaaagcac ctgctgctta tccagctttt gcaccagcgg ctgcaccact ttatccccat
     1861 gctgaccaaa gtattgatac agcgtatcgg acgcactttt ggtcagcacc ataatctcaa
     1921 tgcgtcggtt gcccgcgctt tgcggatttt tcgcgtccag cagcatctgg tccgccatcg
     1981 cgctcacctg catcacttta tcttccggca ttccggcctc ttccagcacc cgacgagccg
     2041 aaagcgcgcg atcgcccgaa aggttccagt tgttgtagat attgtttttg taggccatcg
     2101 catcggtatg cccggtaata ataattttgt tatcgagcga gtcgaacact ggcgcgagct
     2161 ccaccagcaa agttttaaag aacggcataa tctgtgcgct gccgcgttca aacatattgc
     2221 ggttctggtc atctttaatc agcacgcgca gtccctgggg aacaatctcc atctccagat
     2281 tcgcttccat atgtgcatca cgggcaatgg tgttgatact ggtcgccagc tcgccaagct
     2341 cagtagcgga tttcttcttc agcagcgcca ttttctcatt gacgtcacgc gcttttttct
     2401 ccgtctcttc cggcaccgcc acggtggcag gttttggcgg atgagacagg ctgattttat
     2461 ttaacggcga caacccgccg ccattgaaaa tcgactgccc gtgcagcgcg gcgataatgc
     2521 tttcacgctc ggatttactg acgctgttga caatcaactt tatcgtcgtc accaaagggg
     2581 ccgagcgtat ttccgaggtt tctgcccgct ttaccctgga cgcgatgccc ggcaaacaga
     2641 tggcgattga cgccgatctt aacgccgggt tgatcaacca ggcgcaggcg caaacccggc
     2701 gtaaagatgt tgccagcgag gccgatttct acggcgcaat ggacggggca tcgaagtttg
     2761 tgcgcgggga tgccatcgcc gggatgatga ttctggcgat caacctgatc ggcggcgtct
     2821 gtatcgggat cttcaaatac aacctgagcg ccgatgccgc cttccagcaa tatgtgctga
     2881 tgaccatcgg cgacggcctg gtggcgcaga tcccgtccct gctgctttcc accgcggcgg
     2941 cgattatcgt cacccgcgtc agcgacaacg gcgatattgc ccatgacgtg cgtaaccagt
     3001 tgctggcaag cccgtcggtg ctctacaccg ccaccgggat catgtttgtg ctggcggttg
     3061 tgccggggat gccgcatctg ccgtttttgc tgttcagcgc cctgcttggt tttaccggct
     3121 ggcggatgag caaacgcccg caggcggcgg aggcggagga gaaaagcctc gaaacgctga
     3181 cccgcaccat caccgaaacc agcgagcaac aggtcagttg ggagactatt ccgctgatcg
     3241 aacccatcag tttaagcctc ggttacaaac tggtggcgct ggtcgataaa gcgcagggta
     3301 acccactcac ccagcgtatt cgcggcgtgc ggcaagtgat atccgacggc aacggcgtgc
     3361 tgctgccgga gatccgtatt cgggaaaact tccgcctcaa gcccagccag tacgccatat
     3421 tcatcaacgg cattaaggcc gatgaagcgg atattccggc ggataaactg atggccctgc
     3481 cctccagcga aacctacggc gagattgacg gcgtgctggg gaacgacccg gcgtacggga
     3541 tgccggtgac ctggatccag cccgcgcaga aagcgaaagc gctgaatatg gggtatcagg
     3601 tgatcgacag cgccagcgtg atcgctaccc atgtgaacaa gattgtgcgc agctatattc
     3661 ctgatttgtt taactatgat gacatcacgc agttgcataa ccgtttgtcg tcgatggcac
     3721 cgcgcctggc ggaagatttg agcgcggcgc tcaattacag ccaattgctg aaagtgtacc
     3781 gggcgctgct gaccgaaggc gtttccctgc gcgatattgt caccatcgcc accgtgctgg
     3841 tcgccagtag cacagtgacc aaagatcata tcctgctggc ggccgatgtg cgcctggcat
     3901 tgcggcgcag cattacccat ccgttcgttc gcaagcagga gctgacggtg tatacgctga
     3961 ataatgagct ggaaaatctg ctcaccaacg tggtgaatca ggcgcaacag ggcgggaaag
     4021 tgatgctcga cagcgtgccg gtcgatccga atatgctcaa ccagttccag agtacgatgc
     4081 cgcaggtaaa agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg gtgccgccgc
     4141 agttgcgtcc tttgctggcg cgttatgcaa ggttgtttgc gccggggctg catgtgctgt
     4201 cgtataacga agtgccggac gagctggagt tgaagattat gggggcgttg agttgattga
     4261 ggaatctttt ccatgctttg cagcacctga caccattaat tgacgtcaca ctgcaaagag
     4321 actacgatga caccatacaa tgacgtcatg gattgatagt atgattaagg tcagcgcgct
     4381 aggctctcgt catagaaaaa cattcgacga tgtaatgacg gagccgccaa aatctggaat
     4441 taaatgggat gatgttgttg cactcattaa agctgtgggt ggaacaataa aaaataacaa
     4501 cggctccaga cgaaaatttc agattggaac gaccaaattc agcacacatg agccacatcc
     4561 aaagaatgtg atggataaag gcgcagtagc aggtcttaag gagtggtttg ttaactgtgt
     4621 aggggtagat tatgaataaa gcatcgaaca ttatcacaat taaaggtcaa ccagccgcag
     4681 tgacatttga agccgatatc aacgcatttc gcggcaagtt cctcaatgta aacggttatt
     4741 gtgactttgt agccacaagt attgaggcat tatatcgtga aggcgaaagc gcgctggatg
     4801 agtggatggc ggattgtgaa gaggacggta ttgccccttt tcgtgaagaa gaagagcaaa
     4861 aacgcctgac gcttcgtgtt cctcatcaca ttgattcgcg gctgaccatc gtcgccagac
     4921 aacattccat ttcaaaaaac cagctcattg ttgatgtctt agaacgtgag ttttcagccc
     4981 agatgtaaac aggtcggatg ccgcgtggca tccgaccttt tgccagttcg tcaatattac
     5041 ccatttacaa aatcgtctct tccgtcaaaa tccggcgcgc gcctaaaaaa tggtcctgcc
     5101 agaaaggttc agctaaccga cttacccgta tggtttcccc ggtgcgcggc gactcaataa
     5161 actgtccatc gcccagatac acgcccatat ggtcggcaat ctcgcggctg tggatatgaa
     5221 aaaacagcaa atctccccgg cgcaggtcgt tattcgccac aatggttgcc cggcgatagt
     5281 gatacatctc attagccgtg cgtgggagct tcgcctcgag gatcttgttg taggcataaa
     5341 aaaccaaccc gctacagtcg aagccttgat cgggccgcgt gccgccccag acgtaaggct
     5401 tgccaagctg ctgctccagg cggtgaatcg ccacttcggt gatgttgtgt agatggtcgc
     5461 tgcttaagaa atggttgttc tccgccagcg cctgccagcg tctgtcactt gccttgagcg
     5521 gcgcaggaaa ccattctgga tgctgtttta tctgttcgcg gttgtgctgg cgcagcgcct
     5581 ttctgctttc ggcattgcct tccacaatat agctggcctg ttttttcaga tgggtttggt
     5641 actgttttaa taaacgcgca cggttttgca tccgctggcg ggcggcgtag ctgaaagaga
     5701 tatgtgatgt ggtggcgctg gcgagtaaag ggagcaaaac gcataacgaa aacacgcctg
     5761 gatgcagaaa tctgccgagc aagaacgaag acatgaagga cataaaccgg ctgaatgaag
     5821 gtgagaattt taaaagacca gaggagtaaa cgggattatt aattgcttat ttgcatatta
     5881 ctattgaggt ttcataatct taggaaataa atgatacatg cgcttttcgg actattgaaa
     5941 actgcccctg tgctaaattt gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg
     6001 tgataatgtg agccttccca atccagacta ttccgatttg ggatccattt tgccgtcctg
     6061 gcgtgtgtaa ccgttttatc aaggaataag cagtatgcgt aaaatcgcat taattcttgc
     6121 gatgttgttg atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta gctcgaccac
     6181 gccagtgagc aaagagtata agcagcagtt gatgggatcc cctgtctaca tccagatctt
     6241 caaggaagaa cgtacactcg atctctacgt caaaatgggc gagcaatatc aactgctcga
     6301 cagctataaa atctgtaaat attccggcgg cttagggcca aaacagcgtc agggcgattt
     6361 caaaagcccg gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag acagccgtta
     6421 ctacaaagcg attaatattg gattccccaa tgcctatgac cgtgcgcatg gttatgaagg
     6481 gaaatatctg atgatccacg gcgattgtgt ttccatcggc tgctacgcga tgaccaatca
     6541 gggtattgat gagatcttcc agttcgttac tggtgcgctg gtttttggtc agccgagcgt
     6601 gcaagtgagt atttacccgt tccgcatgac cgacgccaat atgaagcgcc ataaatattc
     6661 caactttaag gacttctggg agcaactgaa gccgggctac gactactttg agcaaacccg
     6721 taagccaccg accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc cgttaagcca
     6781 cgaagttgtg caaccacagc tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc
     6841 attcgcctgg cataatcttt tgccaggttt cattgcccgt cagcggctgt gtcgcaatca
     6901 ccgtgaccac atcattcggt gtggtctgcg agctgaagtc gatttccaca tcctgatcca
     6961 gcaacgttgc cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat
     7021 acgccattac atagcgcccg tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt
     7081 catccgccag tgaggcgata tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt
     7141 aacgctgcgt taatttatgc aggagccagc agaaggcttt ttcgctgtcg gtttcaccga
     7201 ccgggcggaa gttgccggtt tccagtgatt tgtatcccgt cagttgtccg ttatgggcat
     7261 aagtccagtt acgcccccat aactcgcggg taaacgggtg agtattttcc agcgccacct
     7321 cgccccgatt cgcctggcga atatgagcca ccaccgaaca ggattttatc gggtagtcct
     7381 ggacaagttt ggcgatgggg gaattaaagc tgggttgcgg atctttaaat gtgcgacagc
     7441 ctttaccttc gtaaaaggta atgccccagc catctttatg tggcccggtt ccaccaccac
     7501 gctgtacaag tccggtgaaa ctaaagcaga tatcggtagg gacgttggcg ctcatcccga
     7561 gcaattcgca cataggtaac ccccacaaca gcgggagcgc atggcccccg gcgaagattg
     7621 atttaagtat tttcaggtcg gatgcggcgt aaacgcatta ttcggcctac gccagactta
     7681 cttaaccatc tctttttcaa ttaactggat caggatatgg atcactttaa tgtggatctc
     7741 ctgaatgcgg tcggcataac caaagtgcgg tacgcgaatt tcgatatccg ctgtgccagc
     7801 cattttaccg ccgtctttac cggtcagggt gatcactttc atccccttct cacgcgccgc
     7861 cgcgatcgct ttgatcacgt ttgcagagtt accggaggtg gagatcccca gtagtacatc
     7921 gccttcgcga cctaccgctt caacgtaacg ggagaaaata tcgttgaaac caaaatcgtt
     7981 accgacgcag gaaatatgac taacgtcaga aatggcaatc gccggatagc ccggacggtt
     8041 ttcacggtag cgccccgtca actcttcggc aaagtgcata gcgtcgcaat gggagccgcc
     8101 gttgccacag gaaagcactt tgccaccagc tttaaaactg tctgctaaca ggaccgccgc
     8161 gcgctgaatg gcgtgaatat tggcgtcatc ttttaaaaag ttagccagcg tttccgccgc
     8221 ttcgttcagt tcgttacgaa taagatcctg gtacatgagg atatccttca gcataaatgt
     8281 aatagacaaa atgcagtgta ccggataccg ccaaaagcga gaagtacggg caggtgctat
     8341 gaccaggact ttttgacctg aagtgcggat aaaaacagca acaatgtgag ctttgttgta
     8401 attatattgt aaacacattg ctaaatattt ttacatccac tacaaccata tcatcacaag
     8461 tggtcagacc tcctacaagt aaggggcttt tcgttatgat gattttgagt attctcgcta
     8521 cggttgtcct gctcggcgcg ttgttctatc accgcgtgag cttatttatc agcagtctga
     8581 ttttgctcgc ctggacagcc gccctcggcg ttgctggtct gtggtcggcg tgggtactgg
     8641 tgccgctggc cattatcctc gtgccgttta actttgcacc tatgcgtaag tcgatgattt
     8701 ccgcgccggt atttcgcggt ttccgtaagg tgatgccgcc gatgtcgcgc actgagaaag
     8761 aagcgattga tgcgggcacc acctggtggg agggcgactt gttccagggc aagccggact
     8821 ggaaaaagct gcataactat ccgcagccgc gcctgaccgc cgaagagcaa gcgtttctcg
     8881 acggcccggt agaagaagcc tgccggatgg cgaatgattt ccagatcacc catgagctgg
     8941 cggatctgcc gccggagttg tgggcgtacc ttaaagagca tcgtttcttc gcgatgatta
     9001 tcaaaaaaga gtacggcggg ctggagttct cggcttatgc ccagtctcgc gtgctgcaaa
     9061 aactctccgg cgtgagcggg atcctggcga ttaccgtcgg cgtgccaaac tcattaggcc
     9121 cgggcgagct gctgcaacat tacggcactg acgagcagaa aaatcactat ctgccgcgtc
     9181 tggcgcgtgg tcaggagatc ccttgctttg cactgacaag ccccgaagcg ggttccgatg
     9241 cgggcgcgat tccggacacc gggattgtct gcatgggcga atggcagggc cagcaggtgc
     9301 tgggcatgcg tctgacctgg aacaagcgtt acattacgct ggcaccgatt gcgactgtgc
     9361 ttggcctggc gtttaaactc tccgacccgg aaaaactgct gggtggcgca gaagatttag
     9421 gtattacctg tgcgctgatc ccgaccacca cgccgggcgt ggaaattggt cgtcgccatt
     9481 tcccgctgaa cgtaccgttc cagaacggac cgacgcgcgg taaagatgtc ttcgtaccga
     9541 tcgattacat tatcggcgga ccaaaaatgg ccgggcaagg ctggcggatg ctggtggagt
     9601 gcctctcggt aggccgcggc atcaccctgc cttccaactc aaccggcggt gtgaaatcgg
     9661 tagcgctggc aacgggcgcg tatgcgcaca ttcgccgtca gttcaaaatt tctattggta
     9721 agatggaagg gattgaagag ccgctggcgc gtattgccgg taatgcctat gtgatggatg
     9781 ctgcggcatc gctgattacc tacggcatta tgctcggtga aaaacctgcc gtgctgtcgg
     9841 ctatcgttaa gtatcactgt acccaccgcg ggcagcagtc gattattgat gcgatggata
     9901 ttaccggcgg taaaggcatt atgctcgggc aaagcaactt cctggcgcgt gcttaccagg
     9961 gcgcaccgat tgccatcacc gttgaagggg ctaacattct gacccgcagc atgatgatct
    10021 tcggacaagg agcgattcgt tgccatccgt acgtgctgga agagatggaa gcggcgaaga
    10081 acaatgacgt taacgcgttc gataaactgc tgttcaaaca tatcggtcac gtcggcagta
    10141 acaaagttcg cagcttctgg ctgggcctga cgcgcggttt aaccagcagc acgccaaccg
    10201 gcgatgccac taaacgctac tatcagcacc tgaaccgcct gagcgccaac ctcgccctgc
    10261 tttctgatgt ctcgatggct gtgctgggcg gcagcctgaa acgtcgtgag cgtatctcgg
    10321 cccgcctggg ggatatttta agccagcttt acctcgcctc tgccgttctg aagcgttatg
    10381 acgacgaagg ccgtaatgaa gccgatctgc cgctggtgca ctggggcgta caggatgcgc
    10441 tgtataaggc tgaacaggcg atggacgatt tactgcaaaa cttcccgaac cgcgtggttg
    10501 ccgggctgct gaatgtagtg atcttcccga ccggacgtca ttatctggca ccgtctgaca
    10561 agctggatca taaagtggcg aagattttac aagtgccgaa cgccacccgt tcccgcattg
    10621 gtcgcggtca gtacctgacg ccgagcgagc ataatccggt tggcttgctg gaagaggcgc
    10681 tggtggatgt gattgccgcc gacccaattc atcagcggat ctgtaaagag ctgggtaaaa
    10741 acctgccgtt tacccgtctg gatgaactgg cgcataacgc gctggcgaag gggctgattg
    10801 ataaagatga agccgctatt ctggtgaaag ctgaagaaag ccgtctgcgc agtattaacg
    10861 ttgatgactt tgatccggaa gagctggcga cgaagccggt aaagttgccg gataaagtgc
    10921 ggaaagttga agccgcgtaa ttgaataacg gagccgaaag gctccgtttc tttatccgct
    10981 aattatttaa aattaaagcc atccggatgg ttttccaggc tgccggtcaa cgccgcgaac
    11041 agcaccgttt taccatcaat cgaaagcgca tcgttcacat tcagccaggt gagtttctct
    11101 tgcgacgttt tctcatcaat agtcgagaac agccccgtca tctgattaga tttctcggac
    11161 cacatcacag cgatacgttg cgagccacag tcatgcggtt tgcacgcgct catcacctga
    11221 tacgtctcat ctcccaacgt tacggtttgt gcgggagtat aagtaccgcc tttcatcacc
    11281 catgcaggta gcttatgccc ttgcaccatc tgattaaacg cagctttggt ggtttcgccc
    11341 tttgcaaggc tgctaatggt taaatcatcc tgcgccattg cactggtggc gatgaccaga
    11401 gcggcgactg tcgttattgc cttaaacatc attcctcccg agcttatcct gcccatgtta
    11461 ttaacctcct aaagcctggc gataacacga taatttgcaa atcagatatt tccaatatca
    11521 gcatttgtta tcaatttatt ctgcgctaag gcaactgcct gataaggtgt tagagttata
    11581 aaacccttcg cataaaggag ttgtacaccg tgtctggttt gaagattacg cttttacagc
    11641 aaccactggt gtggatggat ggtcctgcca acctgcgtca ttttgatcgt caactggaag
    11701 gtattaccgg acgcgatgtg atcgttctac cggagatgtt taccagcggc tttgccatgg
    11761 aagcggcagc ttcgtcgcta gcacaagatg acgtagtgaa ctggatgaca gccaaagcgc
    11821 agcagtgcaa tgcgctgatt gcaggcagtg ttgcattaca aacggagtct ggttcggtta
    11881 accgcttttt gctggttgag ccgggcggca cggtacattt ttatgataag cgtcatctgt
    11941 tccgcatggc agatgagcat ctacattata aagcgggcaa tgcgcgagtg attgtggaat
    12001 ggcgcggctg gcgtattttg ccgctggtgt gctacgactt acgttttcct gtgtggtcgc
    12061 gcaatctcaa cgattatgac ctcgccctat acgtcgccaa ctggcctgct ccgcgctctc
    12121 tgcactggca ggcattgctg acggcccgcg cgattgagaa tcaggcgtat gtggcgggat
    12181 gcaatcgcgt cggcagcgat ggcaacggct gccattatcg cggtgacagc cgggtgatta
    12241 atccacaggg cgagattatc gctactgccg acgcgcatca ggcaacgcgc attgatgcgg
    12301 agctgtcgat ggtggcgttg cgggaatata gagaaaagtt tccggcatgg cgggatgcgg
    12361 atgagtttag gttgtggtga gtgggtgttg tgtttagggg cggggtaaga ctacgggtca
    12421 gggcaagatt acgaaagccc ggagcctgtc catcattctg tgtaaatgcc tttctcagaa
    12481 gtgaccgtca aggcggtcac cgaactcgat aataaagcga ctcatcccta tccgccagtc
    12541 ctgaagtggc atcgtccatt tttgcgacgc tgactggaaa gccagccaca ccactttttt
    12601 caccgactca tctgtcggga acactttccg tttctttatc gcatgtgtga tgatgctatt
    12661 cagcgattcg attgcgttag tggtgtagat aaccttgcgg atcgcctccg ggtacacgaa
    12721 gaatgtggca acattcggcc agtttgcccg ccagctgcga cttatctgcg ggtaccgtgc
    12781 atcccattcc ttcccaggga aagattacga aagcccgctc cccgcaagga ctgacgccag
    12841 atagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    12901 atcattcgtc aaaatattaa tagcgatgtg ccgtatccct gaaaata
//
LOCUS       DARNVM010000138        12906 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.138, whole genome shotgun sequence.
ACCESSION   DARNVM010000138 DARNVM010000000
VERSION     DARNVM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.138"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            417..1106
                     /gene="mutH"
                     /locus_tag="SI621_003584"
     CDS             417..1106
                     /gene="mutH"
                     /locus_tag="SI621_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HEI4066249.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1175..1888
                     /locus_tag="SI621_003585"
     CDS             1175..1888
                     /locus_tag="SI621_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEI4066250.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2026..2244
                     /gene="ygdR"
                     /locus_tag="SI621_003586"
     CDS             2026..2244
                     /gene="ygdR"
                     /locus_tag="SI621_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HEI4066251.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2352..3392
                     /locus_tag="SI621_003587"
     CDS             2352..3392
                     /locus_tag="SI621_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEI4066252.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3424..4617)
                     /gene="lplT"
                     /locus_tag="SI621_003588"
     CDS             complement(3424..4617)
                     /gene="lplT"
                     /locus_tag="SI621_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEI4066253.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLVAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4610..6769)
                     /gene="aas"
                     /locus_tag="SI621_003589"
     CDS             complement(4610..6769)
                     /gene="aas"
                     /locus_tag="SI621_003589"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HEI4066254.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAIYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7355..8386
                     /gene="galR"
                     /locus_tag="SI621_003590"
     CDS             7355..8386
                     /gene="galR"
                     /locus_tag="SI621_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HEI4066255.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8393..9655)
                     /gene="lysA"
                     /locus_tag="SI621_003591"
     CDS             complement(8393..9655)
                     /gene="lysA"
                     /locus_tag="SI621_003591"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEI4066256.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9777..10712
                     /gene="lysR"
                     /locus_tag="SI621_003592"
     CDS             9777..10712
                     /gene="lysR"
                     /locus_tag="SI621_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HEI4066257.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHMPAGTER
                     TELLSLDEVCVLPPGHPLAAKEILTPEDFHSENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10699..11391)
                     /gene="ygeA"
                     /locus_tag="SI621_003593"
     CDS             complement(10699..11391)
                     /gene="ygeA"
                     /locus_tag="SI621_003593"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEI4066258.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11520..>12906)
                     /gene="araE"
                     /locus_tag="SI621_003594"
     CDS             complement(11520..>12906)
                     /gene="araE"
                     /locus_tag="SI621_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HEI4066259.1"
                     /translation="PRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIAGALPFITDHFV
                     LTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLGSIGSAFATSVE
                     MLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLGIVLAFLSDTAF
                     SYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVLRMLRDTSEKAR
                     EELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNIIMYYAPRIFKM
                     AGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVMALGTLVLGYCL
                     MQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKCRDFGITCSTTT
                     NWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETKNVTLEHIERKL
                     MAGEKLRNIGV"
BASE COUNT         3261 a         3481 c         3174 g         2990 t
ORIGIN      
        1 tcaatgcatt acatgtaatt tgagtaataa aaaagagtaa ttcgtgaccc aggtcacacc
       61 tctcatttac gggttggcga aagatcaatc aacgcggtat ttatccacag aatgtgccac
      121 taagttaagc actgagccac taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg
      181 aaacagtagt ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga
      241 tcctgtttat tttcagtgac cagatttgga aaacccgttg cagtgttgcg caactcgatt
      301 accgacaatc taaaaagcaa tataaatcag ataattgaac aacgcctgtg gaaaaagtta
      361 cactgcgaat attcggcaca gaaatgctgt ttgtttttta accaaggtat catgacatgt
      421 cccaacctcg cccactgctc tctcctcccg aaactgaaga acaattgtta gcgcaagcac
      481 aacaactttc tggttataca ttgggagaac tggcggcact tgctgggctg gtgacgccgg
      541 agaatttaaa acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca
      601 gcgcagggag taaacctgag caagattttg ctgccctggg cgtggaactt aaaactatcc
      661 ctgtggatag tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg
      721 gtaatagcgg ggtgacctgg gaaaccagcc acgtacgcca caagctcaaa cgcgtactgt
      781 ggataccggt tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt
      841 tactgtggag cccgaatgaa gaggaagacc ggcagctacg cgaagactgg gaggaattaa
      901 tggatatgat tgttctcggt caggttgagc ggattaccgc tcgtcacggg gaatatttac
      961 agatacgacc gaaagcagcg aatgcgaaag cgcttaccga agccattggt gcccggggcg
     1021 aacggattct gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac
     1081 tggcccgtca ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata
     1141 attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc
     1201 taacgcctgg cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa
     1261 tattattttt ctttctctgg tggtggcaaa gctccccaca gcacaacgtg ctcatgcgcg
     1321 gcgtctggga ttggcgggag ccatggttat gcgtctggcg ctgctggcat ccatcgcctg
     1381 ggttacgcgc ctgacgaatc cgctttttac aatattcagt caggaaattt ccgcccgtga
     1441 tttgattctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga
     1501 atccatcgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat
     1561 cgtgcagatt atgctgctgg atattatctt cagcctcgat tcagtgatta ccgctgtggg
     1621 tctgtcagat cacctgttta ttatgatggc agccgtggta attgccgtag gcgtgatgat
     1681 gttcgctgcg cgttcgattg gtgattttgt cgagcgccat ccttcggtaa aaatgctggc
     1741 gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt
     1801 accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaacct
     1861 tattcgcaac aaaaagaacc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac
     1921 tcactcctgt tcccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt
     1981 tataaacaga ctattatcat aggtgggcca aatgaggata accggatgaa aaaatgggca
     2041 gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc
     2101 atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat
     2161 accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat
     2221 gtttcgcaaa ttattgaacg ttaactaata aggtcagcat ccggctggcc ttaagatttt
     2281 tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa
     2341 aggaaacggc tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg
     2401 ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg
     2461 actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc
     2521 cgcgccccga aacgcaaggg ttgactgaaa cctacgtcgg caactggctg gcgaaacatg
     2581 gcagccgcga aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca
     2641 agggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca
     2701 gcctcaagcg tctgcagact gattacctcg atctctatca ggtgcactgg ccgcagcgcc
     2761 caaccaactg cttcggcaaa ctcggttata gctggacaga ttctgcgcct gcggtttcgc
     2821 tgctggatac gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg
     2881 gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg
     2941 atctgccgcg tattgtcacc attcagaacc cctacagcct gttaaaccgc agttttgaag
     3001 taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg
     3061 gtttcggcac actgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata
     3121 cgctctttag tcgcttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt
     3181 atgttgatat cgccagacgt catggcctgg atcctgctca gatggcgctc gcttttgtac
     3241 gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac cacgatggag cagctgaaaa
     3301 ctaacgtcga aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg
     3361 tacatcaggt ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa
     3421 atattaatga cggcgctgcc aaatccacag cgccgttatt gccagcgcaa acagcgcacc
     3481 gaagccaatg ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc
     3541 cagcatcaac aacatggcgc tgttttcgcc aaggttttgt actgcaattg cattccccgc
     3601 cccgacgctt tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc
     3661 ccccagcacg ccaatcagca tcaacaaggc ataggctggc aacagctcgt gttgcaggga
     3721 gaaaatcagt accaccacac caatcaaaat cccggctggc atacagcgtg acacggtttc
     3781 cagcgtaact aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc
     3841 gttgagatag gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac
     3901 caacaggaaa cgcagcgtga cacctgctcc ccagaataaa ctggtgccca ccagcgaaaa
     3961 acgcgtttca ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt
     4021 gatgagattc caggactgcc ccggacgcgc cgccaccagt ttgggaatgt agatattggc
     4081 aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg gcgaccagga catgccagtc
     4141 agccagcaca ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat
     4201 taaaccgtta gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt
     4261 cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat
     4321 accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg
     4381 gcctttggcg aagctatccg ccacctgtcc gacaaacggc gcaaaaagaa tgtaagcacc
     4441 tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa
     4501 cgccagagtg gcaaacagca gcgcattatc gccaaacgca gagagaaact gcgccacgat
     4561 aaccgctttc atccccttcg accacaacga agtattagtg tgcactgact cactcatcgt
     4621 gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa
     4681 gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt
     4741 gctcgcgggc atactgttgt aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca
     4801 ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg
     4861 aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg
     4921 caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat
     4981 cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt
     5041 ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt
     5101 gcaggcgtcc gccctcttcg ataccaggaa ccgacaacag gcgcgcgtcc atacctggta
     5161 gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg
     5221 cgcactcggt cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct
     5281 gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt
     5341 catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg
     5401 tgcaactgcg gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa
     5461 ggaacacttc tgcacctgtg agcagtggcg taaacaggcc taccgtcagc ccaaaggagt
     5521 gaaacagcgg taacgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa
     5581 tctgctcgac attcgccaga atgcttttat ggctatggac gacgcctttc ggatggcctt
     5641 cagaaccgga ggtaaaaagg atcagcgcct cttcttccgg ctgctgtttt acctgtgcca
     5701 gacgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt
     5761 ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt
     5821 tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag
     5881 cactggtcag cccttttacc ccggcggtgt agttcatcat tgcgggaatg cggcgacggg
     5941 cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc
     6001 gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca
     6061 atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga
     6121 agcggtatat tgcactcagt aaagattcgt acagcgtttc acgcgggcgc accgccatac
     6181 gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac
     6241 gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat atgcagagta atttgcggga
     6301 acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa
     6361 tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgca ccatcgtaga
     6421 ttttcatcag cgagcctgtg gtggtgattc gtccttcagg gaaaatcacc actggtcggc
     6481 cctgttccac cagacgtacc agatgtttaa tcgccatagg ttgcgtcggg tcgagaggaa
     6541 caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa
     6601 cggcaaatac cggacgtacg ggtaaaaaca atgccagcag tatgccatca ataaaagaga
     6661 cgtgattagg cgtgattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc
     6721 gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct
     6781 ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga
     6841 cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg
     6901 tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct
     6961 gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg
     7021 tgtaaattgt cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc
     7081 gcaggctggt gtaattcatg tgctcaaccc gaagttgact tcacctatca atacctctgg
     7141 gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc
     7201 gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc
     7261 acattcccag gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga
     7321 aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg
     7381 actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ccaaagccag
     7441 cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc
     7501 caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctggtcg ttggtgatgt
     7561 ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacactgg
     7621 taatttttta ttgattggca acggttacca caacgaacaa aaagagcgtc aggccattga
     7681 gcaactgatc cgccatcgct gtgctgcgct ggttgtccat gccaaaatga tcccggatgc
     7741 cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc
     7801 tggctttgaa aaccgttgta ttgctctgga cgatcgttac ggtgcctggc tggctacgcg
     7861 tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc
     7921 tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattgcggc
     7981 caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggtgaac aggcaatgac
     8041 cgagcttttg ggacgaggca gaaatttcac tgcggtagcc tgttataacg attcaatggc
     8101 ggcgggcgcg atgggcgtgc tcaatgataa tggtattgat gtaccgggtg agatttcgtt
     8161 aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg
     8221 ttacccaatc gtgacgatgg cgacgcaggc tgccgaactg gctttggcgc tggcggataa
     8281 tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc gccattcagt
     8341 gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccac agttaaagca
     8401 attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccga gcctgaccat
     8461 tatcaaacag aacttctggt aacagcggac gactgttgta gttggatgac attgatgcgc
     8521 catatgcccc cgtatcatgc aataccagat aatcacccgc cttcacttcc ggcaaggcgc
     8581 gggtttcgac atttccccct tcctgctggg taaagacatc gcccgattca cataacggtc
     8641 cggcgacgac ggtttccacc gttggtgcgt gttccagaga acgaccatca gctgccaggg
     8701 cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat
     8761 caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacttga gtaattaata
     8821 cgccagcctg cgctaccaga aagcgacccg gttcaatttc cagtttcaca ggatggccca
     8881 aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa
     8941 ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta
     9001 aatcctgacc gaattcgagg acctgacgca ccatagcacc acacacttgc tccagatggg
     9061 cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga tgatgacgtt
     9121 gtatcacgtc cagtgcggcg ggcagatcgg tataccagat accgtgcttg ctgttttcgc
     9181 caccagtatt ggttttttgg ctatgcccgt gaccaaaccc cggattaaca cgcagccata
     9241 cccgatgccc tggcgaaacc tgacccagtt ggtcgagcat atcaacagaa cccgcattca
     9301 ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa
     9361 aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt
     9421 cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa
     9481 tattggaaca ggctttctgt gcgaagcgca ccacatcaaa ctgtttcagc gctgcaatct
     9541 gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattct gcgggcaaac
     9601 gcagcagatt ttcggcggtg agatcggtat cagtgctgaa cagtgaatgt ggcataacaa
     9661 actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga
     9721 tttatgttga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg
     9781 ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga
     9841 ctgaggcggc acacctgcta cacacctcac agccaactgt cagccgcgaa ctggcgcgct
     9901 ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tggacgattg catcctaccg
     9961 tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg
    10021 tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc
    10081 cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg
    10141 atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc
    10201 agcgtcatga tttaggactc actgagacac tccatatgcc tgcgggaaca gaacgtaccg
    10261 aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggcaaaag
    10321 agatattaac gccagaagat tttcacagtg agaactacat cagcctttcc cgtactgaca
    10381 gctatcgcca gttgctggat caattgttta ctgagaatca ggttaaacga cgcatgatcg
    10441 tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg
    10501 tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg cggcggttca
    10561 gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc
    10621 tggtgcaggc gtttagtgag catttacaag cgggattacc gaaactggtc acttctcttg
    10681 acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc
    10741 cgcggtatca aacacaggca gaacactgcg ctcttctggc accagtaaac caatttctgt
    10801 gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata
    10861 ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt
    10921 aattttcgcc cgttcatccg cttcaggaat aagacagttg atggaaaatt gttccgtcag
    10981 ccgcccgcga taaaaatcct gttccatggt gtaacgtgta cccagcagcg ccacacgagt
    11041 cattcctgcc ccggtaattg cacgtccagt ggcatccgct atgtgtaaga aaggcagaga
    11101 gcaacgagac tcaatggcat ccgccacttt atgcatcgta ttggtgcata acacaatacc
    11161 ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatatccc cggttttgtc
    11221 ccactcaccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcac
    11281 ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta
    11341 aggaatagtg gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc
    11401 ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag
    11461 gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat
    11521 cagacgccga tatttctcaa cttttcgcct gccatcagtt tgcgctcgat atgttccagc
    11581 gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc aatgttcagc
    11641 gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggtc
    11701 gcgccgataa tcatattcga cacccagttt gtcgtcgttg agcaggtgat gccaaaatcg
    11761 cggcatttca gcggctgaat ttcagagcac aagatccaca ccactggcgc ggcgctcatc
    11821 gcataaccgg caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa
    11881 gccgtaccgt tatcaaactg catcaggcag taacccagca ccagagtgcc taacgccatc
    11941 acgctgaaac caattttcag agccggctta cgccctgctt tatctaccgt aaagaccgca
    12001 atgaaggtag cgaacataaa ggtcagcccc acgaccagag tcgcaatcat ctgttgttct
    12061 gtggtcgtaa agcccgccat tttgaagata cgtggcgcgt agtacatgat gatgttcata
    12121 ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa atacagcacg acggacgtta
    12181 cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc acgaatttcg
    12241 ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc
    12301 tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga
    12361 ataatcagca ggactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta
    12421 taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg
    12481 tacatgctga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atatagagga
    12541 gcggtgtaag acgcgatccc aacagcaatg cccaacacca cacgagcggc gattaacatc
    12601 tctacgctgg tcgcaaaagc ggaccctatc gaaccgagta caaacaggat ggcccccgcc
    12661 atcaggctgt atttacgccc caggcggaac gacagccacc cattaaacag cgcaccaatt
    12721 gctgcgccga gcatcatgct actgaccacc cattcctgca aacggctggt cagcacaaag
    12781 tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat
    12841 cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgtgtatc ccgcagagga
    12901 cgtggc
//
LOCUS       DARNVM010000139        12864 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.139, whole genome shotgun sequence.
ACCESSION   DARNVM010000139 DARNVM010000000
VERSION     DARNVM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.139"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            428..802
                     /gene="tomB"
                     /locus_tag="SI621_003595"
     CDS             428..802
                     /gene="tomB"
                     /locus_tag="SI621_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HEI4066260.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            828..1046
                     /gene="hha"
                     /locus_tag="SI621_003596"
     CDS             828..1046
                     /gene="hha"
                     /locus_tag="SI621_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HEI4066261.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1218..1769
                     /gene="maa"
                     /locus_tag="SI621_003597"
     CDS             1218..1769
                     /gene="maa"
                     /locus_tag="SI621_003597"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HEI4066262.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1885..2355
                     /locus_tag="SI621_003598"
     CDS             1885..2355
                     /locus_tag="SI621_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HEI4066263.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2519..4069
                     /gene="pdeB"
                     /locus_tag="SI621_003599"
     CDS             2519..4069
                     /gene="pdeB"
                     /locus_tag="SI621_003599"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HEI4066264.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4111..4464)
                     /locus_tag="SI621_003600"
     CDS             complement(4111..4464)
                     /locus_tag="SI621_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HEI4066265.1"
                     /translation="MKYVDGFVVAVPVDKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4584..4680)
                     /gene="ffs"
                     /locus_tag="SI621_003601"
     ncRNA           complement(4584..4680)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SI621_003601"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4843..5154
                     /locus_tag="SI621_003602"
     CDS             4843..5154
                     /locus_tag="SI621_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEI4066266.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5185..5757)
                     /locus_tag="SI621_003603"
     CDS             complement(5185..5757)
                     /locus_tag="SI621_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEI4066267.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5975..6835
                     /gene="tesB"
                     /locus_tag="SI621_003604"
     CDS             5975..6835
                     /gene="tesB"
                     /locus_tag="SI621_003604"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEI4066268.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6884..8170)
                     /gene="amtB"
                     /locus_tag="SI621_003605"
     CDS             complement(6884..8170)
                     /gene="amtB"
                     /locus_tag="SI621_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HEI4066269.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8200..8538)
                     /gene="glnK"
                     /locus_tag="SI621_003606"
     CDS             complement(8200..8538)
                     /gene="glnK"
                     /locus_tag="SI621_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEI4066270.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADESAL"
     gene            complement(8719..10500)
                     /locus_tag="SI621_003607"
     CDS             complement(8719..10500)
                     /locus_tag="SI621_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEI4066271.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVSM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQSRFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10493..12265)
                     /locus_tag="SI621_003608"
     CDS             complement(10493..12265)
                     /locus_tag="SI621_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEI4066272.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGTANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12295..12753)
                     /gene="decR"
                     /locus_tag="SI621_003609"
     CDS             complement(12295..12753)
                     /gene="decR"
                     /locus_tag="SI621_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HEI4066273.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
BASE COUNT         3261 a         3439 c         3077 g         3087 t
ORIGIN      
        1 ataaaattca taaaaaatac tgattgttag ttaatttata ttaagtagcg ctaataaatt
       61 taataatcca taatcattta gaggctattc ttaattattt gcggtaattc tttattcatt
      121 cctcggttat tacgtcatat tcagagcaat cctggtatta gtgtcaccaa tttcatctgg
      181 cgataatcct gaaatgttat gaatagttcg agcagactgc ttttacctac tgcgggttag
      241 tgctagtatg aaaaaatgag tcctgtcccg attccttcct aattgtaatt tttcgtaata
      301 atgcgatgaa aacctgcaaa gagtggctta tagttaagct aacaaacgag agggcaagtc
      361 caggtcagta agttttttcc atcccgaaag gtgtccgtta gttcaaccac taagaagggg
      421 acgcgttatg gatgaatact cacccaaaag acatgatatc gcacagctta agtttctctg
      481 tgaaaccctg tatcatgact gtcttgcaaa ccttgaagaa agcaatcatg gctgggttaa
      541 cgacccaacc tcggcgatca acctccagtt gaatgaactg attgagcata ttgcgacctt
      601 cgcacttaat tacaaaatta agtataatga agacaataag ctcattgagc agatcgacga
      661 atatctggat gacaccttta tgttgttcag cagttatggt attaatatgc aggatcttca
      721 gaaatggcgg aagtcaggta atcgactatt ccgttgtttt gtcaatgcga cgaaagagaa
      781 tcctgcgagt ttatcttgtt agaattatta caaccatggg tagaagtatg tccgaaaaac
      841 ctttaacaaa aaccgattat ttaatgcgtt tacgtcgttg ccagacaatt gacacgctgg
      901 agcgtgttat cgagaaaaat aaatacgaat tatcagataa tgaactggcg gtattttact
      961 cagccgcaga tcaccgcctc gccgaattga ccatgaataa actgtacgac aagatccctt
     1021 cctcagtatg gaaatttatt cgctaataaa taattcgctt tcggagctat aaccggctgt
     1081 ttattaagaa ttttatactt tttcgccatg aagacgtacc ctatgtgatc tttatcacac
     1141 agatgtaatg ggaacgttct cttcactgac ttttcgtctt actgtgttgc cgcattttca
     1201 gcaaccggag gtcagtaatg agcacagaaa aagaaaagat gattgctggt gagttgtatc
     1261 gctcggcaga tgagacgtta tctcgcgatc gcctgcgcgc tcgtcagctt attcaccgat
     1321 acaatcattc cctggcggaa gagcacacat tacgccagca aattctcgct gatttattcg
     1381 gtcaggtgac agaggcttat attgagccaa cgtttcgctg tgattatggc tataacattt
     1441 ttctcggcaa taattttttc gccaacttcg attgcgtgat gcttgatgtc tgccctattc
     1501 gcatcggtga taactgtatg ttggcaccag gcgttcatat ctacacggca acacatccca
     1561 tcgaccctgt agcacgtaat agcggtgctg aactggggaa acccgtcacc atcggtaata
     1621 acgtctggat tggcggacgc gcggtcatta accctggtgt gaccattggt gataacgtcg
     1681 tggtagcctc aggtgcagtt gtcacaaaag atgtcccgga caacgttgtc gtgggcggta
     1741 atccagccag aataattaaa aaattgtaat aggtttttcg caactgttat gcaaaattgt
     1801 ggtaaatctg ttactttccc tctactattc ccacgttaaa atagggtgtt ccctggaaag
     1861 ttgcagatac cacgaaggca aacgatgacc gaaatacaac gcctgctgac cgaaacaatt
     1921 gagtctctaa atacccgcga aaaacgcgac aacaaacccc gctttagtat cagttttatc
     1981 cgtaaacatc cggggctgtt tatcggtatg tatgttgctt tttttgccac cctggcggtg
     2041 atgttgcagt ccgaaacgct ttcaggctct gtctggctac tggttgtatt atttatcctg
     2101 cttaatggtt tcttcttttt cgatgtctac ccacgctacc gctatgaaga tatcgacgtg
     2161 ctggatttcc gcgtttgcta taacggcgaa tggtacaaca cgcgctttgt acctgccgcg
     2221 ctggttgaag ccatcttgaa ctctccgcgt gtcgcggatg ttcataaaga acaactgcaa
     2281 aaaatgatcg tccgtaaagg tgaactgtct ttttacgata tttttaccct cgctcgcgcc
     2341 gaatcaacat cttaagttag ggttacatac caggcgtaaa gctctgcgcc tggtcaaatg
     2401 acaacgttcg tttccaccca tcccttcatg aaatcccggc tctacctcct tatctcctgc
     2461 aagccttttt ccacaatcag atatactttc cctacactgt gttaataagg atatggtggt
     2521 gagaacacga catctggtcg gccttatttc gggagtactg attctttcag tattgctgcc
     2581 tgtcggctta agcatctggc tggcccatca gcaggtagaa acatcattta ttgaagagct
     2641 gaatacgtat tcctcccgcg tcgctattcg ggccaataag gtggcgacac aagggaaaga
     2701 tgcgctgcaa gagctggaaa gatggcaagg cgctgcctgt agcgaagccc atctcatgga
     2761 aatgcgtcgg gtatcttaca gttatcgcta tattcaggaa gtggtttata tcgataacaa
     2821 cgttccccag tgttcgtctc tggagcatga aagcccgccc gataccttcc ccgagccagg
     2881 taaaatttcg aaagatggtt atcgtgtctg gttaacatcg cataacgatt taggcattat
     2941 ccgttacatg gttgccatgg gaacggcaca ttatgtcgtc atgatcgacc ccgcttcctt
     3001 tattgatgtc attccctata gctcatggca aattgatgcc gccattattg gcaatgccca
     3061 taacgttgtg ataaccagca gcgatgaaat tgctcaggga attattacca gactacaaaa
     3121 aacacccggt gagcatatcg aaaataatgg aatcatttac gatatcctgc ccttcccgga
     3181 aatgaatatt tcgatcatca cctgggcttc aacgaaaatg ttgcagaaag gctggcatcg
     3241 gcaagtcttt atttggttac cgctcgggtt ggtgattggc ctgctggcag cgatgtttgt
     3301 gctgcgtatt ttgcgccgta tccagtcacc gcatcatcgg ctgcaggatg ctatcgaaaa
     3361 tcgtgatatt tgcgtgcact atcagccgat tgtctcctta gccaatggca aaattgtcgg
     3421 tgctgaggca ctggcgcgct ggccgcagac agacggtagt tggttgtcac cagatagttt
     3481 tattccgctg gcacagcaaa cgggcctttc tgagccattg acgctactga ttataagaag
     3541 cgtctttgaa gatatgggcg actggctgcg tcagcatccg cagcagcata tttcgatcaa
     3601 tcttgaatcc accgtgctca cctcggaaaa aatcccgcaa ttgctgcgtg aaatgatcaa
     3661 tcactatcag gttaatccca gacagatcgc gcttgaactc actgaacgcg agtttgccga
     3721 tccgaaaacc agcgccccga taatttctcg ctaccgggag gcgggccatg aaatttatct
     3781 tgatgatttt ggtacggggt attcaagttt aagttattta caggatctgg atgtcgacat
     3841 tctgaagatc gataaatctt tcgttgatgc gctggaatat aaaaatgtca cgccgcatat
     3901 catcgaaatg gcaaaaacac tgaaactgaa aatggtagcg gagggaatcg aaaccagtaa
     3961 acaagaagag tggttacgcc agcatggcgt gcactacggt cagggctggc tctacagcaa
     4021 ggcattaccg aaagaagatt tcttacgctg ggccgagcaa catttgtgaa ttgtagggct
     4081 gacggcacgc cgcacagccc gtcacgctac ctattcgtcg atgattgact cgaatccgcc
     4141 atagatcatt cgcttaccat caaacggcat ggactcgccg aactctttca tccgtgggtc
     4201 cgacatcatc ttttgattag cagcgtcgcg gacctctttt gaagggtatt caatccagct
     4261 aaagacaacc tcttcattct cttcagcttt taccgccata cgaaaatcgg tcactttgcc
     4321 atccggtaca tcgctggccc agcattcgac aatacgaagc gcgccaaact ctttaaacaa
     4381 tggcgcagcc ttagcagcca tttcccgata ggcatccttt ttatcgacag gaacggcaac
     4441 cacaaaacca tcaacatact tcatcagaca tacctccgaa gcgtactgcg cagccagaca
     4501 attcttgcga cgtcagttgt ggttaaagtt tagccatatt gggatttttt gacgaaacgg
     4561 ggggaggcag aaatgggtgg gggccctgcc agctacatcc cggcacacgc gtcatctgcc
     4621 ttggctgctt ccttccggac ctgacctggt aaacagagta gcgttgcggg agaaccaaca
     4681 gagcccccat tgagagcgtt gagaaccaac gcgcaggcgc attatcactg ctgccccggc
     4741 taattgcaac ccattgcgtg ccggatgctg gtttcttgcg caatgcggct tcactcgggc
     4801 gtttttccag attatgctga aaaattacct caggaagaaa agatggaaaa agaagattca
     4861 tttccccaac gcgtctggca aatcgtcgcc gctattcccg aaggctatgt caccacttac
     4921 ggtgatgtgg cgaaactggc aggatcgccc cgcgccgcgc gccaggtggg cggtgtgtta
     4981 aagcgtctcc ccgaaggcag caccttaccc tggcaccggg tggttaatcg ccacggcaca
     5041 atttcgctaa ccggaccgga tttacagcgt caacgacagg cattactggc agaaggtgtg
     5101 atggtatcgg gaagcgggca aatcgacttg cagcgttatc gctggaacta ctaaccctct
     5161 ccgggcggag agggttaact gcgcttaata ttgcgtagga gctggaactg ccgaagacgg
     5221 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
     5281 cggcacggcg gtttgctgca ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
     5341 gatcaccggc tgaacggtat cggtaataaa taccagtttg tcattcacgg taatcgccgc
     5401 actcaacaga atacgcgcat tcggctgaac atctgccggg ttaaatggca gaacaaagct
     5461 gaatggtgac tgtttgcctt cggtacgcac cgctttctgc gccagtactt ttgacggtgc
     5521 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
     5581 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
     5641 tgctgaaata gacgtatttg cagccggtgc tggcgtctga atatccgcgc ttttatctgc
     5701 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
     5761 cttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
     5821 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
     5881 attcagacca ttgcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
     5941 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
     6001 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
     6061 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
     6121 gaccgtccct gaagagcggc tggtacattc gtttcatagc tactttcttc gccctggcga
     6181 tagtaagaag ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     6241 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgactg cctctttcca
     6301 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     6361 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgctgaa
     6421 agataaattc atctgcgatc gtccgcttga agtccgtccg gtggagtttc ataacccact
     6481 gaaagggcac gtcgcagaac cgcatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     6541 ggatgatctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     6601 ggtagctcta cagccgcacg gcatcggttt tctcgaacca ggaattcaga tagcgaccat
     6661 cgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     6721 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccagga
     6781 cggcgtgctg gttgcctcga ccgttcagga aggagtaatg cgtaatcaca attaaaaaaa
     6841 tagccggagg tgaaaaccgt tcggctgttt tttgcagtgc ttgttacgcg ttataggcat
     6901 tttcgccgtg gctgttgacg tcaagccctt ctcgctcctg ttcttccggc acacgcaggc
     6961 caaccgtcag atccgccagt ttgtagccga taaatgccac cacaccggac cagacgatcg
     7021 taatggcgat gctttccagc tgtaccagca actgatggcc catcgtcaca ccttcagcga
     7081 agcccacgcc gcccagcgaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     7141 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     7201 gcatggtaac gccccacaag cccgccagac cagctaccac gccgataatc aacgcgccgc
     7261 caacaccaat gtagccacac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     7321 acgctcccag cagtgaaggc tttccgcgca gcgcccattc accgaagatc cagccaagaa
     7381 ttgctgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     7441 tgcccgctga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     7501 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcccacg cgtttgccta
     7561 tcagatatgc gcccaccaga ccggcgattg cggcgttaat gtgcaccacc gtgccacccg
     7621 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccagaccata tgcgcaatcg
     7681 gaatgtagga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     7741 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     7801 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg tttttcagca
     7861 tcaaccagtt aatgttgccg aagaagttgt ttccctcacc aaacgccagc gagtaaccgt
     7921 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     7981 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     8041 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     8101 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     8161 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg attcgtcggc
     8221 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     8281 aatttttccg gtgtaagccg ctttgctgac gatatcgatc acttcatcga gttggtcatc
     8341 agcaatcgcc acatcaattt ttacttttgg caggaaattg acgctgtatt ccgccccccg
     8401 gtacagctcg gcatgtcctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     8461 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     8521 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     8581 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaggagagc aggaagttaa
     8641 gaagattgaa aacaaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     8701 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     8761 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     8821 ttgctggtga gtaccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     8881 atcaacaatg gtcgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     8941 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     9001 aaggatcagg atttgcggcg tctccaccaa cacgcgtgcc agtgccagca gttgcttttg
     9061 cccgactgag agattattcc cctgctcgcc aagcggcgtg taaataccgt cgctcatgct
     9121 acgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     9181 atcacgcccc agcgtcacgt tggcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     9241 caccattgcc acgccctggc gcagcgcgct gtgacttagc gaacttaatg gacgaccatc
     9301 aaggcgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtgct
     9361 tttgccactg ccggtatgcc cgaccagtgc cacaaagtta cgcgaaggca cagagagatt
     9421 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     9481 ggtgccgctc tgtaacgggc gatcatcatt gccatattgc tggcgcggtc cgtccatcag
     9541 ttcaaacaca cgctcaccag caactacagc ctgttgcagc atcgcctgct gcgtggtcag
     9601 ttcgattaat ggttcgttaa gtcgcccaag atagctgata aatgcataca acacgcccac
     9661 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaac aacccacaaa gaatgagcga
     9721 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     9781 cgccatatag tgtgaacgac tggcctcacc catacgttcg ccaaatcgcg actgctgacg
     9841 gaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatctgc
     9901 cagataagcg cgtacacggc ggacgatcgg cgtgctgtaa cgctgatata tcaccattac
     9961 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
    10021 catcgacacc agcatcgcgc ccaccagtgc ggcgctacgc aggacagttg ccactacggt
    10081 aacgtagagg tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
    10141 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
    10201 caactgttgc actacgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
    10261 cccggcggca aacagttgca gcccaacata cgccgcagcc agccctgcaa ccactttcaa
    10321 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
    10381 gacttctgcc gctgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
    10441 cggcgaaccg tacgctaaca gacgcttgag agtcggccac agttggctaa aactacgcat
    10501 caacggcctc ctcgcgaatt tccggagcgt cgtcgagcgc cgcctccagt tgttgatagc
    10561 gatacatatc gcgataccag ccgctttgtt gcgccagctc atcatgattg ccacgctgag
    10621 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
    10681 gggcactgat gattaccgtt cttccctgcc cccattgacg caggttatgc aggatctggt
    10741 gctctgtgcg tccgtctact gccgaaagcg catcatcaag gatgaggatt tccgcgttga
    10801 ctaataacgc acgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
    10861 gctcgcccac ttctgtatcg taaccttgcg gtaaacgcaa aatatcgtca tgcacgctgg
    10921 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc aacgcgatgt
    10981 tgttcgctac ggtgtcggaa aaaaggaacg gtgtctggct aactaccgcc aggcggctac
    11041 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcggata tccccctcgc
    11101 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
    11161 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
    11221 ggtcagtctg cggatacgtg aactggcgaa tatttacatc cagttcgcca cgaccttccg
    11281 gcacgggttc gctaccatca ttcaccaccg gcgcttccgc cagcatcgcg cgaatgcggc
    11341 tgtacgcagc actaccacgt tccactatgt taaacatcca tgccagcgcc agcattggcc
    11401 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactaccct
    11461 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccgtgccg atggcgatgt
    11521 aaatggtcgg atcaaaacgg gcatcaatac gtgccacacg catgtttttc ttgccggtat
    11581 cttcggcatc ctcggcaaat aacgccgact ggcgatcttc cagaccaaaa gctttgatca
    11641 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actagaaaac gccgcctgtg
    11701 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt gatcatgatc gccatcactg
    11761 gcatcggcaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
    11821 acacagcgca gcccatcacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
    11881 acacgacgcg atcgacgtca ttggtcgcac gagccatgag gtcacccgtg cgatgacgca
    11941 ggtaaaactc aggatgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
    12001 ccagttgata agacgcacca aacagcaata cgcgccagac gtaacgcagg agataaacca
    12061 caacggcaat cagcaccatg gtggcgatcc acatcaggat ctgcccggta gtaaagtgtt
    12121 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
    12181 cgataatgac aagcaaggcg acagccccga gatagcgacg ccattcccga cggaaatacc
    12241 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
    12301 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
    12361 cagccccggc acgctgttta ccaggcgctt ataaaactcg tcgtagcgtt tcatgtcggc
    12421 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaacc ccagcacttc
    12481 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
    12541 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
    12601 tttgccgata agaataccgt cgtcctccag ccgtttcaga cgcttccagc agggggtagt
    12661 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgtagtaa
    12721 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
    12781 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
    12841 ggcacaaaat gacaaattca acgc
//
LOCUS       DARNVM010000140        12860 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.140, whole genome shotgun sequence.
ACCESSION   DARNVM010000140 DARNVM010000000
VERSION     DARNVM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.140"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            168..503
                     /locus_tag="SI621_003610"
     CDS             168..503
                     /locus_tag="SI621_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HEI4066274.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            663..1106
                     /gene="yjdN"
                     /locus_tag="SI621_003611"
     CDS             663..1106
                     /gene="yjdN"
                     /locus_tag="SI621_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HEI4066275.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AKNCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1239..2027
                     /gene="phnC"
                     /locus_tag="SI621_003612"
     CDS             1239..2027
                     /gene="phnC"
                     /locus_tag="SI621_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEI4066276.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2052..3068
                     /gene="phnD"
                     /locus_tag="SI621_003613"
     CDS             2052..3068
                     /gene="phnD"
                     /locus_tag="SI621_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4066277.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3129..3908
                     /gene="phnE"
                     /locus_tag="SI621_003614"
     CDS             3129..3908
                     /gene="phnE"
                     /locus_tag="SI621_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEI4066278.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3929..4654
                     /gene="phnF"
                     /locus_tag="SI621_003615"
     CDS             3929..4654
                     /gene="phnF"
                     /locus_tag="SI621_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEI4066279.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4655..5107
                     /gene="phnG"
                     /locus_tag="SI621_003616"
     CDS             4655..5107
                     /gene="phnG"
                     /locus_tag="SI621_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEI4066280.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRATVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5104..5688
                     /gene="phnH"
                     /locus_tag="SI621_003617"
     CDS             5104..5688
                     /gene="phnH"
                     /locus_tag="SI621_003617"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEI4066281.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5688..6752
                     /gene="phnI"
                     /locus_tag="SI621_003618"
     CDS             5688..6752
                     /gene="phnI"
                     /locus_tag="SI621_003618"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HEI4066282.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSAQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLITECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6745..7590
                     /gene="phnJ"
                     /locus_tag="SI621_003619"
     CDS             6745..7590
                     /gene="phnJ"
                     /locus_tag="SI621_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEI4066283.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7587..8345
                     /gene="phnK"
                     /locus_tag="SI621_003620"
     CDS             7587..8345
                     /gene="phnK"
                     /locus_tag="SI621_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEI4066284.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPSNRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8456..9136
                     /gene="phnL"
                     /locus_tag="SI621_003621"
     CDS             8456..9136
                     /gene="phnL"
                     /locus_tag="SI621_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEI4066285.1"
                     /translation="MINVQNVSKTFILHQQNGVHLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9133..10269
                     /gene="phnM"
                     /locus_tag="SI621_003622"
     CDS             9133..10269
                     /gene="phnM"
                     /locus_tag="SI621_003622"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEI4066286.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRRHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLTQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10269..10826
                     /gene="phnN"
                     /locus_tag="SI621_003623"
     CDS             10269..10826
                     /gene="phnN"
                     /locus_tag="SI621_003623"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HEI4066287.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGIGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10813..11247
                     /gene="phnO"
                     /locus_tag="SI621_003624"
     CDS             10813..11247
                     /gene="phnO"
                     /locus_tag="SI621_003624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HEI4066288.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11249..12007
                     /gene="phnP"
                     /locus_tag="SI621_003625"
     CDS             11249..12007
                     /gene="phnP"
                     /locus_tag="SI621_003625"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HEI4066289.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12166..12495)
                     /gene="yjdP"
                     /locus_tag="SI621_003626"
     CDS             complement(12166..12495)
                     /gene="yjdP"
                     /locus_tag="SI621_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HEI4066290.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12564..12776)
                     /locus_tag="SI621_003627"
     CDS             complement(12564..12776)
                     /locus_tag="SI621_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEI4066291.1"
                     /translation="MSEGIDKETRSSERTDYPHYASEVALAFGSRVVGLELAKMIVDA
                     WLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2794 a         3881 c         3633 g         2552 t
ORIGIN      
        1 gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat
       61 cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt ttcggcaatc
      121 tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg tcattaccac
      181 actgcccaaa atgcaactcc gaatacacct acgaagataa cggcatgtac atctgcccgg
      241 aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag ctgatcgtta
      301 aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt aaagatctga
      361 aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg
      421 ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg aaactgaaat
      481 ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa ggcgctcgcg
      541 ccgcatccgg caacgatgcc gactgcctga tgcgacgctg accgcgtctt atcaggcttc
      601 cctctcatct gtataaattt cgaactacac ttaagtggct tctcttaact gaggtcacca
      661 tcatgccgtt aagtccctac ctctcttttg ccggtaactg tgccgacgca attgcttatt
      721 atcaacgtac gttgggcgct gaactgctct ataaaatcag cttcggcgaa atgccaaaat
      781 cagcgcagga cagcgccaag aactgccctt ccggaatgca atttcccgat accgccatcg
      841 cccacgccaa tgtgcgcatt gccggaagcg atatcatgat gagcgatgct ataccgtcag
      901 gaaaagccag ctactccggc tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa
      961 aacgctggtt tgacaacctt gccgctaacg gaaaaatcga aatggcctgg caggaaactt
     1021 tctgggcgca tggcttcggc aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg
     1081 tcgtcaaaca acaaccaacg cactaacccg ccgggagggc cgccctcccg cactgtcatc
     1141 gaaatctcgt taactcttca tccgttagtc acttttagtt aacaaaatcg tcacaataat
     1201 ccgccacgat ggagccactt ttttagggag gctgcatcat gcaaacgatt atccgtgtcg
     1261 agaagctcgc caaaaccttc aatcagcatc aggcgctgca cgcggttgat ctgaacattc
     1321 atcacggtga aatggtggct ctgcttgggc cgtcgggttc cggcaaatcc acccttttac
     1381 gtcacttaag cggtttgatt accggcgata aatctgccgg tagccatatc gagctgctgg
     1441 gccgcacagt ccagcgcgaa ggccgcctgg cccgcgatat ccgcaaaagc cgcgccaaca
     1501 ccggctacat cttccaacaa ttcaacctgg tgaatcgcct gagcgtactg gagaacgtgc
     1561 tgattggcgc gctcggcagc acgccattct ggcgcacctg ttttagctac ttcactcgcg
     1621 agcagaaaca gcgcgcgtta caggcgctga cccgcgttgg catggtgcac tttgcccatc
     1681 agcgcgtttc caccctctcc ggcggacagc agcagcgtgt ggcgattgcc cgtgcgctga
     1741 tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc ctcgctggac ccggaatcag
     1801 cgcgcatcgt gatggacacc ctgcgcgaca tcaaccagaa cgacggcatc accgtggtcg
     1861 tcacgctgca tcaggtggat tacgccctgc gctactgcga acgcatcgtc gccctgcgtc
     1921 aggggcacgt cttctacgac ggcagcagcc aacagtttga taacgaacgc tttgatcatc
     1981 tctatcgcag catcaaccgc gtcgaagaga acgcgaaagc tgcctgacat ccccatcatt
     2041 gaggaaaacg aatgaacgct aagataattg cctcgctggc cttcaccagc atgttcagcc
     2101 tcagcaccct gttaagcccg gcgcacgccg aagagcagga aaaggcgctg aatttcggca
     2161 ttatttcaac ggaatcacag caaaacctga aaccgcaatg gacgccgttc ttacaggata
     2221 tggagaagaa gctgggcgta aaggtcaacg ccttctttgc cccggactac gcgggcatta
     2281 tccaggggat gcgcttcaat aaagtggata tcgcctggta cggcaatctg tcggcgatgg
     2341 aagcggtgga tcgcgccaac ggtcaggtct tcgcccagac ggtcgcagcg gatggatcgc
     2401 cgggttactg gagcgtgttg atcgtcaaca aagacagtcc gatcaacaac ctgaacgatc
     2461 tgctggcgaa gcgtaaagat ctcaccttcg gcaatggcga tcctaactcc acctctggct
     2521 tcctcgtccc cggctactac gtcttcgcca aaaacaatat ctccgccagc gacttcaagc
     2581 gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt cgccaacaag caggtggatg
     2641 tggcgaccaa caacaccgaa aacctcgaca agctgaaaac ctccgcgcca gagaagctca
     2701 aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg cgatccgatc gtctggcgca
     2761 agaatctttc cgaaaccacc aaagacaaga tctacgactt ctttatgaat tacggcaaaa
     2821 cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc cccgttccgc gcctccagcg
     2881 acctgcaact ggtgccgatt cgccagctcg cgctgtttaa agagatgcag ggcgtgaaag
     2941 gcaataaagg gctgaatgag caggacaagc tggcaaaaac tagcgccatt caggcgcaac
     3001 tggatgacct ggaccgcctg aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg
     3061 tgcagtaatg tgctcgatcg gtcccctctc ccacagggag agggagaaaa cattaaacgg
     3121 agcaatatat gcaaaccatc accatcgccc cacccaagcg cagctggttc tcgcttctga
     3181 gctgggccat tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa atggccccgc
     3241 tcacgctgat taaagacggc ggcaacatgg cgacgttcgc tgccgacttc ttcccgcccg
     3301 atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa atcgccgtct
     3361 ggggcaccgc gctggcggtg gttctctcca tccccttcgg tctgatgagc gccgaaaacc
     3421 tggtgccgtg gtgggtttac cagcccgtac gccgcctgat ggacgcctgc cgcgccatta
     3481 acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg ccttggcccg ttcgctggcg
     3541 tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc gaagcggtgg
     3601 aagcgattga acccggcccg gtggaaggca ttcgcgccac cggtgccaac aagctggaag
     3661 agatcctcta cggcgtgctg ccacaggtga tgccgctgct gatctcctac tccctctatc
     3721 gcttcgaatc caacgtccgt tcggccaccg ttgttggcat ggtcggcgct ggcgggatcg
     3781 gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc gccctgatgg
     3841 tgctgattat cgtcacggtc agcctgctgg atttcctctc tcaacggttg cgtaagcact
     3901 ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga ccagctaccc
     3961 aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac actaccgctg
     4021 cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga accgccacac
     4081 cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc gtcagggcgt
     4141 cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg cgcgttttag
     4201 ccagaacctg ctggatcagg gcagccatcc caccagcgaa aaactgctgt ctgtgctgcg
     4261 cccggcctcc ggtcacgtcg ctgacgcgct ggggataacc gagggcgaga acgttatcca
     4321 cctgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc actactttgc
     4381 ggacctcacc ctctggccga cgctgcaacg cttcgacagc ggctcgctgc acgattttct
     4441 gcgcgagcaa acggggattg cgctgcgccg cagccagacg cggatcagcg cccgccgcgc
     4501 ccaggccaaa gagtgccagc gtcttgaaat cccgaatatg tcgccgctgc tgtgcgtgcg
     4561 cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca gcctgacgcg
     4621 cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg cgactcgcca
     4681 gcactggatg tccgtactgg cacacagcca accggctgaa ctggcagccc gcctgaaggc
     4741 gctgaacatc actgccgact atgaagtaat ccgcgccgct gaaaccggcc tggtgcagat
     4801 tcaggcgcgg atgggtggca ccggcgaacg cttttttgct ggcgacgcca cgctcacccg
     4861 cgccaccgta cgcctgactg acggcacgct cggctacagc tgggtgctgg ggcgcgacaa
     4921 acagcacgcc gaacgctgcg cgctgattga cgcgctgatg cagcaaaccc gccattttca
     4981 aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac gcattgccgc
     5041 acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc gcggagataa
     5101 cgcatgaccc tggaaaccgc ttttatgctt cctgtgcagg atgcccagca cagttttcgt
     5161 cgcctgttaa aggccatgag cgagccgggc gtgattgtcg ccctgcatca gctcaaacgc
     5221 ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc cgataacgac
     5281 acgccggtgt ggctttctgc gccattaagt aacgatatcg tcagccagag cctgcgtttt
     5341 cataccaacg cgccgctggt caatcagccg gaactggcga ccttcgcggt aacggatgag
     5401 gcgatttcca gcgaacagct taacgccctt tccagcggca ccgctgttgc gccagaagcg
     5461 ggcgcgacgc tgattttaca ggtcgtcagc ctgagcggcg ggcgcatgct gcgccttact
     5521 ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg cattctgcac
     5581 gaacttaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct gacctgcggc
     5641 gagcgcctgc tggctatccc gcgaaccact catgtggagg tgtgctgatg tacgttgccg
     5701 tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc cgacgccggg
     5761 gcaataccga tttgcctgaa ctgagcgtcg cccagattga acagcagctt aatcttgcag
     5821 tagatcgcgt gatgaccgaa ggcggcattg ccgaccgcga actggcggcg ctggcgctga
     5881 aacaggccag cggcgataac gtcgaagcga ttttcctgct acgcgcctac cgcaccacgc
     5941 tggcgaagct ggcggtaagc gagccgctcg acaccacgga gatgcgtctc gaacggcgta
     6001 tttcagcggt ttataaagat attcccggcg gccagctgct tggcccaacc tacgactaca
     6061 cccatcgcct gctcgatttc accctgctgg caaacggcga agcgccgacg ctgaccaccg
     6121 ccgacagcgc acaacagccg tcgccgcacg ttttcagcct gctggcgcgt caggggctgg
     6181 cgaagtttga agaggatagc ggcgcacagc cggatgacat cacccgcacg ccgccggttt
     6241 acccctgctc acgttcttcc cgtttgcagc agctcatgcg cggagacgaa ggctatttgc
     6301 tggcgctggc ctactccacc caacgcggtt acggacgcaa tcatccgttc gcaggcgaaa
     6361 tccgcagcgg ctatatcgac gtgtcgattg tgccggaaga gctgggattt gcggtgaacg
     6421 tcggcgaact gctgataact gagtgtgaaa tggtcaacgg ttttatcgac ccgccggatg
     6481 agccgccgca cttcacgcgc ggctacgggc tggtgttcgg catgagcgag cgcaaagcga
     6541 tggcaatggc gctggttgac cgcgctctac aagccccgga gtacggcgag cacgcgacag
     6601 gcccggcaca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa gccgcaggct
     6661 ttgtctcgca cctcaaactc ccccactacg tcgatttcca ggccgaactg gagctactca
     6721 aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt tgcttacctc
     6781 gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc gatccccggt
     6841 tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccat acggctgggg aactggtggt
     6901 atccagctta ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat tgaccagggt
     6961 gcggatgaca ccaccaacgc cgtgtcgatt cgcaacttct ttaagcgcgt gaccggggta
     7021 aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgtcaccg catccccgaa
     7081 acgccgctga ccgaagatca gatcattatc ttccaggtgc caatcccgga accgctgcgc
     7141 tttatcgagc cgcgcgaaac ggagacccgc actatgcacg cgctggagga gtacggcgtg
     7201 atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac cacctatgct
     7261 tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc gaaattcgat
     7321 aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg cgagaagcgc
     7381 atctatgcgg tgccgccgtt tacccgcgtg gaaagcctcg atttcgacga tcacccgttc
     7441 accgttcagc agtggaatga gccatgcgcc atctgcggat cgacccacag ctatcttgat
     7501 gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac cgattattgc
     7561 cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc aataacctga
     7621 cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta tggccggggg
     7681 aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg aaatcaattt
     7741 ccgcgcgtct gacgccgcag caaggggaga ttcactatga aaaccgttcc ctctatggca
     7801 tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg catcagcatc
     7861 cactcgacgg cctgcgccgc caggtgtcgg caggcggcaa tatcggcgag cggctgatgg
     7921 cgaccggggc gcgtcattac ggcgatattc gcgccaccgc gcagaagtgg ctggaggagg
     7981 tggagattcc ctccaaccgg atcgacgacc tgccgaccac cttttccggc ggtatgcagc
     8041 aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt atggatgaac
     8101 cgaccggcgg gctggatgta tcggtacagg cgcgcctgct cgatctgctg cgcggtctgg
     8161 tggtggagct gaatctggcg gtggtgattg tcacccacga tctgggcgtc gcccgcttgc
     8221 tggcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg ttaaccgacc
     8281 gcgtgctcga cgaccctcac catccatata cccagctgct ggtgtcatcg gttttgcaga
     8341 attgagccag tgccggatac ggcgtaaacg ccttatccgg ccgacaaatg cgctccccgt
     8401 aggtcggata agacgcgtca gcgtcgcatc cgacacccga acaacgaggc gaaaaatgat
     8461 taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg tgcacctgcc
     8521 cgtcctcaac cgtgcctcgc tcaccgtcaa cgcaggcgaa tgcgtggtgc ttcacggcca
     8581 ctccggcagc ggaaaatcaa ccctgctgcg ctcgctgtac gccaactatc tgcccgacga
     8641 agggcaaatc cagatcaaac acggtgagga atgggtagac ctggtcaccg cgccagcccg
     8701 caaagtggtg gagatccgca aaaccaccat cggctgggtg agccagtttc tgcgcgtcat
     8761 cccgcgtatt tcggcactgg aagtggtgat gcagcctctg ctcgataccg gcgtaccgcg
     8821 tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc ctgaacgcct
     8881 gtggcacctg gcaccatcga ccttctccgg cggcgaacag cagcgcgtca acatcgcccg
     8941 tggctttatc gtcgactatc cgattctgct gctcgacgaa cctaccgcct cgctggacgc
     9001 taaaaacagc gccgccgtag tggaactgat ccgcgaagcc aaagcccgtg gcgcggccat
     9061 cgtcggtatc ttccatgacg aagccgtacg caatgacgtc gccgaccgcc tgcacccgat
     9121 gggagcctct tcatgattat caataacgtc aaactggtgc tggaaaatga agtggtgcac
     9181 ggttcactgg aagtacagga tggcgaaatc cgcgcctttg ccgaaagcca gagccgccag
     9241 ccggaagcga tggacggcga aggtggctgg ctactgccgg ggctgattga gctgcatacc
     9301 gataatctgg ataaattctt caccccgcgc ccgaaggtcg actggcccgc ccactcggcg
     9361 atgagcagcc acgatgcgct gatggtggcg agcggcatca ccaccgtgct ggacgcggtg
     9421 gcgattggcg acgtgcgcga cggcggcgat cggctggaga atctggagaa gatgatcaac
     9481 gccattgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgcct gcacctgcgc
     9541 tgcgaactgc cgcatcacac cacgctgcca ctgtttgaaa aactggtgca gcgcgagccg
     9601 gtgacgctgg tgtcgctgat ggatcactcg ccgggacagc gccagttcgc caaccgcgag
     9661 aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat gcagcagtac
     9721 gaagaagagc aactggcgct tgccgcacgc tggtcacagc ctaatcgcga atccatcgcc
     9781 gccctgtgcc gcgcgcgaca aattgccctc gccagccacg atgacgccac gcacgcccac
     9841 gttgccgaat cccaccagct tggcagcgtg atcgccgaat ttcccaccac gtttgaagcg
     9901 gcggaagctt cgcgcaggca tggtatgaac gtgctgatgg gcgcgccgaa tatcgtgcgc
     9961 ggcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg cctgctggat
    10021 atcctctctt ccgactacta ccccgccagc ctgctcgatg cggcatttcg cgtcgccgac
    10081 gacgagagca accgctttac gctgacgcag gcggtgaagc tggtgaccaa aaatccggcc
    10141 caggcgctca atcttcagga tcgcggggtg attggggagg gcaaacgcgc cgacctggtg
    10201 ctggcgcatc gcaagggcaa ccacattcat atcgaccacg tctggcgtca gggtaaaagg
    10261 gtgttctgat gacgggaaaa ctgatttggt taatgggggc ctctggctcc ggaaaagaca
    10321 gtctgttgac ggaactccgc cagcgggaac aaactcagct actggtagcg catcgctaca
    10381 tcacgcgcgc cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag caggagtttt
    10441 ttacccgcgc ggggcagaac cttctggcct taagctggca cgccaacggc ctgtattatg
    10501 gcatcggcgt cgagattgac ctctggctgc acgctggatt tgacgtggtg gtcaacggct
    10561 cacgcgccca tctgccgcag gcgcgggcgc gctatcaatc ggcgctgctg cccgtttgtt
    10621 tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt gaaaatgcca
    10681 gtgaaattaa cgcccgactg gcacgcgctg cccgctatac tcctcaggat tgtcttacgc
    10741 tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg attcatcaga
    10801 aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga caccgacgca
    10861 gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccacgc gtttcgcgtg
    10921 gggtttaacg ccaatctgcg cgacccgaac atgcgctacc atttggcgct gctggatggc
    10981 gaagttgtcg gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt caactggatc
    11041 ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacat cggcagtaaa
    11101 ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac cgagctttcc
    11161 accaacgtga agcgccacga cgcgcaccgt ttctatctgc gcgaaggcta cgagcagagc
    11221 catttccgct tcaccaaggc gctgtaacat gagcctgacc ctcacactta ctggcactgg
    11281 cggcgcacag ggcgttcccg catggggctg tgagtgcgcg gcctgcgcca gagcgcggcg
    11341 ctcaccgcag tatcgccgcc agccatgcag cggcgtcgtg aagtttaacg acgcgatcac
    11401 cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat cgttccagca
    11461 gtttttgctg acgcactacc atatggatca cgtgcagggg ctgtttccgc tgcgctgggg
    11521 cgtgggcgat cagatcccgg tctacggccc gccggatgaa cagggctgcg acgatctgtt
    11581 taaacatccg ggcctgcttg atttcagcca cacgatggaa ccgtttgtgg tgtttgattt
    11641 acaggggtta caggtcacgc ccctgccgct caaccactca aaactcacct tcggctattt
    11701 gctggaaacc gcgcacagcc gcgtggcgtg gctgtctgac accgcaggcc taccggaaaa
    11761 aacgctgaaa tttttacgca ataatcaacc gcaggtgatg gtgattgatt gcagccatcc
    11821 gccgcgcgag gatgcgccac gtaatcactg cgatttaaac accgtgctgg cactgaatga
    11881 ggttatccgc tcgccacggg tgattctgac gcatatcagc caccagtttg atgcgtggct
    11941 gatggaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg agattggggt
    12001 ggcgtgatgg gagggaatgt gcgcgctggc cccctcaccc taaccctctc cccagagggg
    12061 cgaggggacc gattgtgctc gatattgaat attgcgctgg ttttctccct cgccccattg
    12121 gggagagggc cggggtgagg ggcgatgcct gctccatacc caacatcaac gcccatactc
    12181 atcttccatt ctccgctctt catcttctag ctgccgacgc tcctgatcaa gctggcgctg
    12241 gcgatcgtcc agctgcctgc ggcgatcttc aaactggcgg cggcgatcgt catattgtct
    12301 gcgccgatcg tcgttcactt cacgctgcca tccgtcgtcg cgcgaatctt catagtctcg
    12361 cccacggtca gggttataag catcgttaat cgcctgctga atattgccaa tggtgtcgtc
    12421 gataatatcg gcctgggccg gaacggtgga cagcgtgagc agggtgaata aaagaaatag
    12481 cggaaagcgt ttcattagcc aacctcaaaa agaaactcta tccacattag tcattactca
    12541 tccatgcaag tagtggatga atctcaattt ctccgctgct ctattgccgt aatcgcctcc
    12601 acgcgttgtt gatgacgacc gccttcgtac tgcgcgccca gccacgcatc cacaatcatt
    12661 tttgccagtt cgaggccaac cactcgtgaa ccaaaagcca gtgcaacttc actggcgtaa
    12721 tgcggataat cagtgcgctc tgacgacctg gtttctttat caatcccctc actcattgac
    12781 catgaataaa aggctggcac agcaacgcag aatcctgcgg tgccagcgac atgaccgccc
    12841 attgctccgg tacgtttttt
//
LOCUS       DARNVM010000141        12750 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.141, whole genome shotgun sequence.
ACCESSION   DARNVM010000141 DARNVM010000000
VERSION     DARNVM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.141"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            210..1268
                     /gene="citC"
                     /locus_tag="SI621_003628"
     CDS             210..1268
                     /gene="citC"
                     /locus_tag="SI621_003628"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEI4066292.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1283..1579
                     /gene="citD"
                     /locus_tag="SI621_003629"
     CDS             1283..1579
                     /gene="citD"
                     /locus_tag="SI621_003629"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEI4066293.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1576..2484
                     /gene="citE"
                     /locus_tag="SI621_003630"
     CDS             1576..2484
                     /gene="citE"
                     /locus_tag="SI621_003630"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEI4066294.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2495..4027
                     /gene="citF"
                     /locus_tag="SI621_003631"
     CDS             2495..4027
                     /gene="citF"
                     /locus_tag="SI621_003631"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEI4066295.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVTPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4031..4582
                     /gene="citX"
                     /locus_tag="SI621_003632"
     CDS             4031..4582
                     /gene="citX"
                     /locus_tag="SI621_003632"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEI4066296.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4557..5435
                     /gene="citG"
                     /locus_tag="SI621_003633"
     CDS             4557..5435
                     /gene="citG"
                     /locus_tag="SI621_003633"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEI4066297.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEEVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSITPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5485..6948
                     /gene="citT"
                     /locus_tag="SI621_003634"
     CDS             5485..6948
                     /gene="citT"
                     /locus_tag="SI621_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HEI4066298.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7062..7868
                     /gene="rna"
                     /locus_tag="SI621_003635"
     CDS             7062..7868
                     /gene="rna"
                     /locus_tag="SI621_003635"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HEI4066299.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQFGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8098..8508
                     /gene="rnk"
                     /locus_tag="SI621_003636"
     CDS             8098..8508
                     /gene="rnk"
                     /locus_tag="SI621_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEI4066300.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8864..9292
                     /gene="uspG"
                     /locus_tag="SI621_003637"
     CDS             8864..9292
                     /gene="uspG"
                     /locus_tag="SI621_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HEI4066301.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9413..10978)
                     /gene="ahpF"
                     /locus_tag="SI621_003638"
     CDS             complement(9413..10978)
                     /gene="ahpF"
                     /locus_tag="SI621_003638"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEI4066302.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVIGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11149..11712)
                     /gene="ahpC"
                     /locus_tag="SI621_003639"
     CDS             complement(11149..11712)
                     /gene="ahpC"
                     /locus_tag="SI621_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEI4066303.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12084..>12750
                     /gene="dsbG"
                     /locus_tag="SI621_003640"
     CDS             12084..>12750
                     /gene="dsbG"
                     /locus_tag="SI621_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEI4066304.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDALVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYY"
BASE COUNT         3013 a         3363 c         3235 g         3139 t
ORIGIN      
        1 ttaaataaat aaaaaaccac taactctatg tgaaataaat caaaatttca cgctgcaata
       61 ctccttagga tgtatagcga aaggagaaaa agatatacct cgttcgcccc cctttctccc
      121 tagtgaaaat aaaaggttat cagtttgcaa catcgaacaa cattcgttgc aaatcgataa
      181 caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc acccgtgtaa
      241 aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga
      301 gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt
      361 gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg
      421 aaggactggc gctgacatta gccaccgagc taattaacct cgcctatgag cggcacagca
      481 cgcatctgtt tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt
      541 ccacgctgac cagtgtaccc ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga
      601 aacgctatgc cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt ggctgcattg
      661 tgatgaacgc caatcccttt acgaatggtc accgttatct gattcaacag gctgcagcac
      721 agtgcgactg gttacatctg tttttagtta aagaagattc ttcacgcttt ccctatgaag
      781 accggctgga tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg
      841 gctccgaata catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg
      901 tcattaacca ttgttacacc gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg
      961 cgctgggtgt aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt
     1021 acaaccagga tatgcgctac tggctggaaa cgccgactat ctccgcaccg cccatcgaac
     1081 tggttgaaat tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc
     1141 aactgctggc gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc
     1201 attatttgca gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc
     1261 ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga
     1321 gtctggggat gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat
     1381 caacagcagc gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct
     1441 cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggcg cactggactg
     1501 cattttacgt gcacgactgg aagccctgct ggcacgcgcc agcggtatcc cggctctgcc
     1561 atgggaggat tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc
     1621 agcatgttgt ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt catctacccg
     1681 gctgatgccc tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc
     1741 cgccgcatgg tttaccacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg
     1801 cgtgtaaacg cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt
     1861 ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc aggatgttct ggatatcgaa
     1921 aaagagatcc tgcgtatcga aaaagcctgt ggtcgtgaac ccggcagtac cggcttgctg
     1981 gcggcgattg aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag
     2041 cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc
     2101 tccccggaag gaactgaact gctgttcgct cgctgctcca ttttgcaggc cgcgcgctct
     2161 gcgggtattc aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg
     2221 caagaagccg cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt
     2281 cagattgatc tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccgc
     2341 cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac
     2401 ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa
     2461 ctttccggca tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca
     2521 acgacaagaa cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca
     2581 gaactcgcca aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa
     2641 cctggaagaa gcgattcgtc gctctggttt acaggacggc atgacggttt ccttccatca
     2701 cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg
     2761 ctttaaaaac ctgaccctgg cctccagctc cctgagtgat tgccatgcgc cgctggtaga
     2821 acacattcgc cagggcgtgg ttacccgcat ttatacctct ggcctgcgcg gtccactggc
     2881 ggaagaaatc tcccgcggtc tgctggcgga accggtgcaa atccactctc acggcggacg
     2941 tgtgcatctg gtacagagcg gcgaactgaa tattgatgtg gctttcctcg gcgtcccatc
     3001 ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg gctccctcgg
     3061 ctatgcaatg gttgatgccg acaacgcgaa acaggttgtg atgctcactg aagaactgct
     3121 gccttatccg cataatccgg caagcattga gcaagatcag gttgatctga tcgtcaaagt
     3181 tgatcgcgtt ggcgatgccg cgaaaatcgg cgctggcgcg acccgtatga ccactaaccc
     3241 gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt gtcaactcag gctactttaa
     3301 agaaggtttc tccatgcaga ccggcaccgg cggcgcatcg ctggcggtaa cccgtttcct
     3361 ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc gcccttggcg gtattaccgc
     3421 aacgatggtt gatctgcacg aaaaagggct gatccgcaaa ctgctggatg tacagagctt
     3481 tgacagccac gctgcacaat cgctggcccg taaccccaat cacatcgaaa tcagcgccaa
     3541 ccagtacgct aactggggtt caaaaggcgc atcggttgat cgtctcgacg tggtggtact
     3601 gagcgcgctg gaaattgaca cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt
     3661 actgcgtggt gcttccggtg gtcactgcga taccgcgatt gcctctgcgc tttccatcat
     3721 cgtcacgccg ctggtacgcg gtcgtattcc gactctggtg gataacgtgc tgacctgcat
     3781 caccccgggc tctagtgtcg atattctggt cactgaccac ggtatcgcag ttaacccggc
     3841 acgtccggag ctggcagaac gtctgcagga agcgggcatt aaagtggttt ccattgagtg
     3901 gctgcgtgaa cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg
     3961 cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa
     4021 ggaataagcc atgcacctgc ttcctgaact cgccagccac catgcggtat caattcccga
     4081 gctgctcgtc agccgggatg aaaggcaagc acggcaacac gtctggctca agcgccatcc
     4141 tgttccactg gtctccttta ccgtggttgc gcctgggccg attaaagaca gcgaggtcac
     4201 acgccgaatt tttaatcatg gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg
     4261 gcaaattcag gagcaggctg cactggtttc cgccagcggg ccggagggca tgttgagcat
     4321 tgccgccccg gctcgcgacc tcaagctcgc caccattgag cttgaacata gtcatcctct
     4381 cgggcggtta tgggatatcg atgtcctgac acccgaaggc gaaattctct cccgccgcga
     4441 ctattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcagctg tttgcgcgcg
     4501 tggaaaaacc catcaactga ccgatttact caaccgcatg gaggcgctgc tgaacgatgt
     4561 cgatgcctgc aacgtcaact aaaaacacaa agcttgcgac gtcattaatc gatgagtacg
     4621 ccctgctggg ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa ccaggcctcg
     4681 tggatcgcat taactgcggt gcgcataaag atatggcgct ggaagatttc caccgcagcg
     4741 cgctggcgat tcagggctgg ttaccccgtt tcattgaatt tggtgcctgt agtgctgaaa
     4801 tggcaccaga agaggtactc cacggattac gcccaatagg tatggcttgc gaaggtgata
     4861 tgttccgcgc cactgcgggc gtaaacacgc ataaaggcag cattttttct ttagggctac
     4921 tgtgtgcggc aattggccgt ttgcttcaac tcaaccagtc gataacgcca ataacaattt
     4981 gtgcgacggc ggcaagtttc tgccgtggcc tgaccgatcg cgaactgcgt accaataatt
     5041 cacaactgac ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc gcacgcggtg
     5101 aagccgaagc gggttatcca ctggtgatca accacgccct gccgcattac ctcactctgc
     5161 tggatcaggg gttagatcct gaactggcac tgctcgatac cttactccta ctgatggcga
     5221 tcaacggcga taccaacgtt gcatcgcgcg gtggcgaggg gggcctgcgc tggctacagc
     5281 gcgaggcgca aacattattg caaaaagggg gcatccgaac ccccgccgat ctcgattatc
     5341 tccggcagtt cgacagggag tgtatcgaac gaaatctcag tccaggcggc agtgccgacc
     5401 tgctgatcct tacttggttt ttagcacaga tttaattatt taagcacttg ataaaattgg
     5461 aatattaatt gtcggagaac ccgtatgtct ttagcaaaag ataatatatg gaaattattg
     5521 gcgcccctgg tggtgatggg tgtcatgttt cttatccctg tacctgacgg tatgccgccg
     5581 caggcgtggc attactttgc agtgtttgtg gcaatgattg tcggcatgat cctcgagcca
     5641 attccggcaa cggcgatcag ttttattgcg gttactattt gcgttattgg cagtaattac
     5701 ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa acaggcgctg
     5761 aaatggggtc tggctggttt ttccagcacc acggtatggc tggtatttgg cgcatttatt
     5821 tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa
     5881 ttcatgggca aacgcacgct gacgttgggt tatgcgattg tcattatcga cattctgctg
     5941 gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc ggtcattaaa
     6001 aacctgccgc cgctgtttaa atcattcccg aacgatccgt ccgcgcgtcg tattggcggc
     6061 tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt tgtcaccggt
     6121 gcggcaccaa acgtactggg tctggagttc gtcagcaaaa ttgccggtat ccagattagc
     6181 tggttgcagt ggttcctctg cttcctgccg gttggggtta tcttgcttat cattgcgccg
     6241 tggctttcct acgtgctgta caaaccggaa atcacacaca gtgaagaagt ggcaacctgg
     6301 gcgggtgatg aactaaaaac catgggtgcg ctgacacgca gagagtggac gctgattggt
     6361 cttgtattgc tcagcttagg tttgtgggta tttggcagtg aagtcattaa tgctactgcg
     6421 gtaggtctgc tggcagtttc gctaatgctg gccctgcacg ttgtgccgtg gaaagacatt
     6481 acccgctata acagcgcatg gaacacactg gtcaacctgg caactctggt tgtgatggct
     6541 aatggcctga ctcgttctgg ttttattgac tggttcgccg gtaccatgag tacgcacctg
     6601 gaaggattct caccaaacgc aacggtgatt gtactggttc tggtgttcta ctttgcgcac
     6661 tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat tctggccgtc
     6721 ggtaaaggta ttccgggcgt accaatggaa caactgtgta tcctgctggt gctgtctatc
     6781 ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat ttacggctgt
     6841 ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt gatttacatc
     6901 tctatgttgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata cgttcgccaa
     6961 acgctgaata aatcatcacg cccgccaggt aagcccacct ggcgggcttt tttatgattt
     7021 aatagatagt attcagtgta tgtatgagtt ccacacccat tatgaaagca ttctggcgta
     7081 acgccgcgtt gctcgcggtt tctctgcttc ccttctcttc tgccaacgcc gtagcgttgc
     7141 aggcaaaaca gtttggcgat tttgatcgct atgtcctggc cctctcctgg caaaccggat
     7201 tttgccagag tcaacacgat cgaaatcgta acgaacgaga tgaatgtcgc ctgcaaaccg
     7261 aaacgaccaa caaagctgat tttctgaccg tacatggtct gtggccagga ttgcctaaat
     7321 cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg cttcggttgc gctactcgcc
     7381 caatcccgaa tctaccagaa gcgcacgcca gccgaatgtg ttcatcgccg gaaaccgggt
     7441 tatcgctgga aacagccgct aaactaagtg aagtcatgcc aggagctggc ggacgttcct
     7501 gcctggaacg ctacgaatat gccaaacacg gtgcctgctt tggttttgat ccagacgcat
     7561 acttcggcac gatggtacgc ctgaatcaag aaattaaaga gagcgaagcc ggaaaattcc
     7621 ttgcggataa ctacggtaag acagtgagcc gccgtgactt tgacgccgcc tttgccaaaa
     7681 gctggggaaa agagaacgtg aaagcagtta agctaacgtg tcagggtaac cctgcgtatt
     7741 tgactgaaat tcagatctcg atcaaagctg acgccatcaa cgctccgctt tctgcaaact
     7801 cattcttgcc acaaccacac ccaggcaact gtggcaaaac ctttgtgatt gataaagcag
     7861 gttattaatc ctctgattta caaaagacgc taattcgtct tccataaacc atgacgatta
     7921 agatttatac ataatgcgct acattgagga attccctgaa tgtgacgcaa atcacttcaa
     7981 agagtgagca acagtcagta tcatcatgtg agttaaaccc tcgccgcctg acggtgaggg
     8041 ttttcttttg ggtatgttta tctgcgacac tcgcagtacc gacgacatgg agtaaaaatg
     8101 tccagaccaa ctatcatcat taacgacctg gatgccgaac gcatcgatat tctgctggag
     8161 caacccgcct atgctggttt gccaatcgcc gacgcgttaa acgcagagtt ggatcgcgcc
     8221 caaatgtgtt cgccagaaga gatgccacac gacgtggtga caatgaacag ccgggttaaa
     8281 ttccgcaatc ttagcgatgg cgaagtgcgt gtgcgcacgc tggtgtatcc ggcaaaaatg
     8341 accgatagca atactcagct ttccgttatg gctccggtag gtgccgcact gctggggctg
     8401 cgcgttggcg attccattca ctgggaactt ccgggcggcg ttgcaaccca ccttgaagtg
     8461 ctggaactcg aataccagcc agaagctgct ggcgactacc tgctttaatc ccatctgcac
     8521 aagcgataac tcgctttgcc cagaggatgc actcgcatcc tctttccgct caaatatcaa
     8581 ccagatttat cattttgctc ttaagcgcag cattctaatc tgaactggtt attcatctgc
     8641 ggaacctcat atgagcgaag ctctcggtat cactgcattt tatatattca tagccgcaat
     8701 tattgttgct gcagtgctat accttgaaca acgctggtag cgcctggttt tatttatttt
     8761 ccagctcaat agcaaattca gatcaaatca gcctcgcaaa ttatagattc ataaccaata
     8821 atgtgctgtt atgtatacat gcaaaaacaa ggagagaatg cttatgtaca aaacaatcat
     8881 catgccagtg gatgttttcg aaatggaatt gagcgacaaa gcggttcgcc acgctgaatt
     8941 cctcgcccag gatgacggag ttattcatct acttcacgta ctaccggggt cagccagcct
     9001 gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc
     9061 agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc gcattaagca
     9121 acatgtccgt tttggtagtg tgcgggatga agttaacgag ttagcgaaag aacttgatgc
     9181 agatgtagta gtgattggtt cgcgcaaccc atcgatttcg acccatctgt taggttctaa
     9241 cgcctcaagc gttatccgcc acgccaatct gccggtgctg gttgtgcgtt aattgccaga
     9301 ataaatatcc cgccctgccc ggagccatcc cggcggggct ttttattgcc ataaaaaagc
     9361 cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct tcttatgcag
     9421 ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct tcgccagtgg
     9481 cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac acgcctttca
     9541 cgttggtttc gcatttcgca tcaatgataa tctcgcccat gcggttacgt tcgactgcgc
     9601 cttcgagcca gttggtgttc ggcagcagac caatctgcac gaaaataccg gccagttcga
     9661 tgttgtgaat atcgccgctg acacgatctc gatattccag accaatgact ttgctgccgt
     9721 cgcctttcac ttccgtagtt tgcgcattca gaataatgtc gacgtttttc aggctgcgca
     9781 gtttgtcctg cagaacctgg tcggctttca tttctggcgc aaattccagc agcgttacgt
     9841 gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg ccgccgataa
     9901 ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt
     9961 tggtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca ccggtcgcca
    10021 cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctga tgcagaccac
    10081 cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt
    10141 attcatcaac gtgaactttc agcgctcctg ccagcttctg cccttcagtc ttcggtacag
    10201 aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt tcgcccatca
    10261 gaccagtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc gctgggccgg
    10321 aaccgacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc gcacgttttt
    10381 ccgcgccagt atcaattttg gcgacgattt cagtcaacgt catgcgaccc tgaccaaact
    10441 ctttcccgtt tacgaatact gccggaacgc ccatcacgtt gcgatcggtg atttcgttct
    10501 ggaaggtacc tccgtcaatt gcagtgtgct tgatgcgcgg gttcagtacg ctcatcaggt
    10561 tcagcgcctg caccacatcc gggcagttgt ggcaagagag cgagtaatag gtttcgaatt
    10621 caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct ttcgacggat
    10681 gaccaccggt ccacaacaac gccagtacca gcgaggtaaa ctcgtggccc agcggggaac
    10741 ctgcaaaacg tggcccctgg ttagaacctg ggttggtgat caggaaagac ggcttacgca
    10801 ccggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg atttcagcca
    10861 acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt aactcaacag
    10921 gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg tcgagcatgt
    10981 ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc tggatgcagc
    11041 ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat taggtgaatt
    11101 tccgaagtat ttcgcgccgg agttggtagc gcgaaagacg aaggaaattt agattttacc
    11161 aaccaggtcc agagacggag ccagagttgc ttcaccttct ttccatttag ccgggcaaac
    11221 ttcacctggg tgagaagcta cgtactgtgc tgctttgatt ttacgcagca ggtcagacgc
    11281 gtcacggcca atgccttcag cggtaacttc gattgcctgg atgataccct gcgggtcaac
    11341 aacgaaggtc gcacggtcag ccagaccttc atcttcacgc atgttgtcga agttacgggt
    11401 cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt ttagcgatgg tttcagagct
    11461 gctgtgccat gctttgtggg tgaagtgagt atcggtagat actgcgtata cgtctacgcc
    11521 cagtttctgc agttcttcgt agtggtcagc aacgtcaccc agttcggtcg ggcatacgaa
    11581 agtaaagtca gccgggtaga agaagaagac gctccagcgg ccttcggtat ctttttcggt
    11641 gatttcgatg aattcgccgt ttttgaatgc ctggttttta aaaggtttaa ttttggtgtt
    11701 aatcaaggac atctatactt cctccgtgtt ttcgatgaga tgtaaggtaa ccaatttttg
    11761 ccgattcggc taatgcgttt ccattatcaa atcgataagt tttaccttac aaccttcgta
    11821 agacaacttt gtgaactttg caccgccagc ggcgataaaa agtaaaatgg ccccctttcc
    11881 aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag cgcctgcctg caggctgagg
    11941 tgttgccctt acaaatgcaa caacgacatg gattacaaca ccctcataaa caaagggcaa
    12001 tcacctgatc taagctctta cctatggcag tgataggtta tgccttttac ccgacttttg
    12061 cactgactga aaaggacaaa ttaatgttaa aaaagatact tttactggct ctgcttcctg
    12121 caatcgcctt cgcagaggaa cttcctgccc cagtaaaagc gattgaaaaa cagggcatta
    12181 caatcatcaa aacgttcgat gctcccggag gaatgaaagg ttatctcgga aagtatcagg
    12241 atatgggcgt caccatctac ctgactccag atggtaagca cgctatctcc ggttacatgt
    12301 acaacgagaa aggtgaaaac ctgagtaaca cacttatcga aaaagaaatt tacgcaccag
    12361 ccggacgcga aatgtggcaa cggatggaac aatcccactg gctcctcgac ggtaaaaaag
    12421 acgcgctggt cattgtttac gtcttcgccg atccgttctg cccatattgt aaacagttct
    12481 ggcagcaggc gcgcccgtgg gtagattctg gcaaagtgca attaagaaca ttgttggttg
    12541 gggttatcaa gccagaaagc ccggcgacag cagcggcaat tcttgcctcc aaagatcccg
    12601 caaaaacctg gcaagaatat gaagcctctg gtggcaagct taagctaaac gtgcctgcaa
    12661 acgtaagtac agagcaaatg aaagtgttaa gcgacaacga gaaactgatg gacgatctgg
    12721 gggcaaatgt cacaccagct atctattaca
//
LOCUS       DARNVM010000142        12661 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.142, whole genome shotgun sequence.
ACCESSION   DARNVM010000142 DARNVM010000000
VERSION     DARNVM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.142"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(319..1410)
                     /gene="ychF"
                     /locus_tag="SI621_003641"
     CDS             complement(319..1410)
                     /gene="ychF"
                     /locus_tag="SI621_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEI4066305.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKTAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1527..2111)
                     /gene="pth"
                     /locus_tag="SI621_003642"
     CDS             complement(1527..2111)
                     /gene="pth"
                     /locus_tag="SI621_003642"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEI4066306.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2389..2667
                     /gene="ychH"
                     /locus_tag="SI621_003643"
     CDS             2389..2667
                     /gene="ychH"
                     /locus_tag="SI621_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HEI4066307.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2722..4401)
                     /gene="dauA"
                     /locus_tag="SI621_003644"
     CDS             complement(2722..4401)
                     /gene="dauA"
                     /locus_tag="SI621_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEI4066308.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4526..5473)
                     /gene="prs"
                     /locus_tag="SI621_003645"
     CDS             complement(4526..5473)
                     /gene="prs"
                     /locus_tag="SI621_003645"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEI4066309.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5624..6475)
                     /gene="ispE"
                     /locus_tag="SI621_003646"
     CDS             complement(5624..6475)
                     /gene="ispE"
                     /locus_tag="SI621_003646"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEI4066310.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6475..7098)
                     /gene="lolB"
                     /locus_tag="SI621_003647"
     CDS             complement(6475..7098)
                     /gene="lolB"
                     /locus_tag="SI621_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HEI4066311.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7312..8568
                     /gene="hemA"
                     /locus_tag="SI621_003648"
     CDS             7312..8568
                     /gene="hemA"
                     /locus_tag="SI621_003648"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEI4066312.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8610..9692
                     /gene="prfA"
                     /locus_tag="SI621_003649"
     CDS             8610..9692
                     /gene="prfA"
                     /locus_tag="SI621_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEI4066313.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDVVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9692..10525
                     /gene="prmC"
                     /locus_tag="SI621_003650"
     CDS             9692..10525
                     /gene="prmC"
                     /locus_tag="SI621_003650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEI4066314.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10522..10914
                     /locus_tag="SI621_003651"
     CDS             10522..10914
                     /locus_tag="SI621_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HEI4066315.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIAFDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10918..11727
                     /locus_tag="SI621_003652"
     CDS             10918..11727
                     /locus_tag="SI621_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEI4066316.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11763..12617
                     /gene="kdsA"
                     /locus_tag="SI621_003653"
     CDS             11763..12617
                     /gene="kdsA"
                     /locus_tag="SI621_003653"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEI4066317.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3151 a         3183 c         3297 g         3030 t
ORIGIN      
        1 gggatattac ccttcaggta agtccttttc tcgccagcat attggagcgt gatcaatatt
       61 gatcagctgt aaaaaggtgt ctgatgtgca gtggaacaca aacttacgtt atctcattgc
      121 taatgagata tccctcatct gaacacttat gtctttaatt attctcgtgg ttcactatag
      181 gcaataagca caaaagtgta ggatgttata agaatggtta ggactcggtg aaatgaaaaa
      241 tccacgcaat tgcgtggatt ttatatactt ttgcgctctt catgagattt agcgaaacct
      301 catgagacaa caaattaatt agacgttgaa caggaagttc attacatcgc catctttaac
      361 gatgtaatct ttgccttctg cacgcatttt gcctgcttct ttcgcgcctt gttcaccttt
      421 gtaagtgatg aaatcttcaa acgagatggt ttgtgcacgg ataaagcctt tttcaaaatc
      481 agtatggatt ttgccagcgg cctgcggcgc ggttgcacca accggaatgg tccatgcacg
      541 tacttctttc accccagcgg tgaagtaagt ttgcaggttc agcagcttat aaccggcacg
      601 gatcacacgg ttcaggcccg gctcttccag cccaagctcc tgcataaact cgtcacgttc
      661 ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca caaaccggaa ccacaacaga
      721 accttctttc gccgcgattt cacgcacctg gtcaagatac gggttgtttt caaaaccgtc
      781 ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc aggaagctca ggtaacgaat
      841 agccgttttc tcttcagcgc ttaaatccag cgcgcgcagc atacctgcgt tttccagctg
      901 cggcaggcat ttttccagta ccgccagctc ggctttcgcg tctttatcgc cacctttggc
      961 tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg aggtccgcca gcgccagttc
     1021 ggtgttgata acttcaatat catcagccgg gttaactttg ccggaaacgt gaatgatgtt
     1081 gtcattttca aagcagcgaa caacgtgacc gatcgcttca gtttcacgga tgttggtcag
     1141 gaactggtta cccagacctt cgcctttcga tgcgcctttt accagaccgg cgatatcgac
     1201 aaattccatg gtcgtgggaa gcgtacgctg cggttttacg atttcagcca gttgatccag
     1261 gcgagggtca ggcattggta cgacgcctgt gttcggctca atggtgcaga atggaaagtt
     1321 ggccgcttca ataccggctt tggtcagcgc gttgaacagg gtagatttcc cgacgttggg
     1381 caaaccgacg ataccgcatt tgaatcccat gatttaaatc accttaatat cttaataatc
     1441 aacctgttat tgctaacaga ttgcagaaat ggaaataact ttgcctatta tacacggcac
     1501 tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa aggcgtgcaa tcggttcgtt
     1561 gctttggtca agccatctgt aaaccacatt tcagtacaac gcgccgcttc gtcgatggct
     1621 tcatcaatta acttctgttc actaacaggc ggtttgccta atacaaaacc aacaacttta
     1681 tttttatcgc ccggatgacc gattccgatg cgtaaacggt gaaagttagg gttattaccc
     1741 aatttactga tgatgtcttt cagtccattg tgaccaccat ggccaccgcc caacttaaat
     1801 ttggcgacgc caggaggcag atccagttcg tcgtgggcca ccagaatttc gtccggatta
     1861 atgcggaaaa aactggccat cgccgcaacg gctttgccgc tgagattcat aaatgtagtc
     1921 gggactaaca ggcggacatc ttcgcctcca agggtgactc gcgaagtata accaaagaat
     1981 ttagcctctt cgcgcagcgg agcgcgcaat cgctctgcca gtaagtcaac gaaccaggca
     2041 ccagcattat gtcgcgttgc ggcgtattca gcgccggggt tcgccaggcc gacaatcaat
     2101 ttaatcgtca cgtttttttg tcctgagtgt gtacataact ggcgcgtagt ttactggttg
     2161 cggccccgct tgacaaaaaa ctgcgtatca aatgcagata acgcaataat tgcctgagtg
     2221 gactattaga aagtcaaggt gttcaggcgt ttatttgtaa agttttgttg aaataagggt
     2281 tgtaattgtg atcacgcccg cacataaccc acagggtgtt gtctatactt tacacataag
     2341 gaagaggggt attccctgtt acaacccaga aagttccgga ggtgacatat gaaacgcaaa
     2401 aacgcttcgt tactcggtaa cgtgctcatg gggttgggtc tggtggtaat ggtggtcggc
     2461 gtggggtatt caatcctcaa ccagttacca cagtttaata tgccccagta tttcgcacat
     2521 ggtgcagtgc taagtatttt cgtcggtgcc attctctggc tggcgggtgc ccgtgttggc
     2581 gggcatgaac aggtgtgcga ccgttactgg tgggttcgcc actatgacaa acgttgccgc
     2641 cgtagcgata atcgccgtca tagctaacaa ataatgcagt ttgctgacca gtcagtttgc
     2701 gctgactggt caatctcgta cttataaatc cgccatcgcc gcgcgacgat tcgggaagaa
     2761 tgcgaggcgt cccgggatcg gttgaatgcc tgcgcgagcc atagtgcgca gtggctggaa
     2821 ttccaggttg cacacgcgca gttcacatcc ttcgggcaga cgctttacaa aacgctggaa
     2881 cgcatcaaga ccaccagcat caagtaccgg aacggcatcc cacttcagaa tcacaatccg
     2941 tttgccttca agacgtgact ccaggtccgt gaacaagcct tcagcggcag caaaaaacag
     3001 cggaccaata acgcgcagaa ccagaacatc gtctggaacg tctacgacta ccggtgccag
     3061 gcgagtcata cgtgcgatac gacgcataaa cagtagcgat gccagcacga tccccacgct
     3121 gatggcaata accatatcaa acagcacagt cagcgacatg cacagcagca tgacgatgat
     3181 gtcatctttc ggcgcatgac gcagcaagtc gaccactttg tgcgcttcac tcatgttcca
     3241 cgccaccatc aacagcaggg cagccatggc ggaaagcggc agccaggaga gcagcggtgc
     3301 cagtaccagc agggcaagaa taaccagaat agagtggatc accgccgaga taggggaagt
     3361 tgccccggca cggacgttag cggcagaacg cgcgatggca gctgtagcgg taataccacc
     3421 aaagaacggg gcgatgatat tccccagtcc ctgtccaacc agttcgctgt tcgctttgtg
     3481 tttcgttccg gtcataccat ccagtaccac ggcgcagagc agagattcga ttgcgccgag
     3541 cattgccatt gagaatgccg caggcagcag tgtgcgaata gaatcccagg tgagggtaaa
     3601 ttctgaattg ggcaggtccc acggtagtac cagttgcggc agcagttgcg gaataccgtt
     3661 accctgagaa ccatctgcca gaacgtagtg gaattgcgat ccgatggtag caacatgccc
     3721 gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc agcaaggccg gaaggtgacc
     3781 aggcaaacga atgcccagac gcggccagaa aaccagaatg ccaagcgtca caatgccaat
     3841 ggcagcatca cccacattaa tggtcggcaa cgccataaat aatgcgccga ctttttgtag
     3901 ataatgctcc gggacatggg ccatttgcag accgagaaaa tctttaatct gcatggtacc
     3961 gatggtgatc ccgatacccg aggtgaaacc taaggtcacg gaaaccggaa tatattcaat
     4021 caagcgtcca aaacgtgcca gccccatcag gatcaaaaag acacctgaca tgagtgtcgc
     4081 gaccagcagt cctgccagtc caaactgttg cgaaacggga tagagaatta ccacaaatgc
     4141 cgcagtcgga ccggaaacgc taaagcgtga cccacccgtc agagcaatga caatccccgc
     4201 aacagctgcg gtatataaac cgtactgggg tgccacacca ctaccaatag ccaacgccat
     4261 tgccagcggg atagcaataa tcccgacggt tatcccggca atcaggtcac gggtaaaccg
     4321 tgcggcagta tatttttctt tccagcaagc gtcgatcaga gcgcggaaag gcatcacatg
     4381 tgaggaaaat attttgttca caataatgtt tcatccgtga gcgcatcatc tgtcaactaa
     4441 atggcaggtg aaggaggcat aggtcataca aatggatatt acagacaaaa aaacccgccg
     4501 cagcgggtct ttgagccggg ttcgattagt gttcgaacat ggcagagatc gattcttcgt
     4561 tgctgatacg acgaatcgct tcggccagca tacctgacag ggtcagagta cgcacgttcg
     4621 gcagtgattt gatttcatcg ctcagcggaa tggtatcgca gacaacgact tcatcaatta
     4681 cagagttacg caggttgttc gccgcgttgc cagagaagat cgggtgagtc gcgtacgcaa
     4741 atacacgttt agcaccacgt tctttcagag cttcagcagc tttacacagc gtaccgccag
     4801 tatcgatcat atcatcgacc agtacgcagt cacgacctgc aacgtcaccg atgatatgca
     4861 tcacctggga aacgttcgca cgcggacgac gtttgtcgat gattgccata tcggtatcgt
     4921 tcagcagctt agcgatagcg cgggcacgca caacgccgcc gatgtccgga gaaaccacaa
     4981 tagggttatc cagattcagc tgcagcatgt cttccagcag gatcgggcta ccaaatacgt
     5041 tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc gtgcagatcc actgtcagca
     5101 cacggtcaac accgacgctg gagaggaagt ctgcaaccac tttcgcagtg attggtacac
     5161 gagcggaacg gacgcgacgg tcctggcgcg catagccaaa gtaggggata acagcggtga
     5221 tacgacctgc ggaagcacga cgcagggcat caaccataac gactaattcc atcaggttgt
     5281 cgttagtagg ggcacaagtg gactggatga tgaaaatatc accaccgcgt acattttcgt
     5341 taatttgtac gctgacttcg ccgtcgctaa agcgacctac agcggcgtcg ccgagtgaag
     5401 tgtacaggcg gttggcaata cgttgtgcta gttccggggt ggcgttacca gcaaaaagct
     5461 tcatatcagg cacgagaaga acctcaggca tgcgtccatt ggtggaaaga atctgccgaa
     5521 aactgtgcgg gccaggcatg attctttcca ggcggtgtat taaagagcgc gatgcaacgt
     5581 ctggaacaag gtgacgttgt caccgaaact cagcttgccc ggcttaaagc atggctctgt
     5641 gcaatgggga aagattaacg cctttcgcca caaagccatt gagccattcc ggggcttgct
     5701 ctagcacctg gcgggcttca gactctgtat caaattcagc aaagacacag gcccctgtcc
     5761 cagtcaggcg cgacggggcg tattctaaca gccaggaaag caccgcatca acctcgcgaa
     5821 aacgttttct tgcgataacc tcgcaatcat tgctgaattc acattttagc aacgtttcta
     5881 ttgacctttt tggcgtattg cgcgggagtt caggatcttt aaaaatcact ggtgtcggaa
     5941 tacttacacc agggtgcgcc accagatacc acttctctgg cggatccacc ggcgttagta
     6001 tttcaccaac gccttcggca aacgcggcat gcccccgaac aaagacagga acatctgcgc
     6061 ccagcgtcag ccccatttcc gccagctcat ccatgcttag cccgcattgc cagagatgat
     6121 ttaatgccac caggaccgtc gcggcattgg atgaaccacc gccgagaccg ccgcccatcg
     6181 gcaaacgctt gtcaatgctg atatccgcac cgcttcccgt cggaagacgc ccgctgtctg
     6241 ccgcagtttt catcaacaat cgtgctgcgc gaacgatcag gttatcttca tgttccacgc
     6301 cttcaacggg cgttaacaga cgaatatccc catcgtcacg aagctcaatg ctgatggtgt
     6361 cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg gtaaccatcc gcacgctgac
     6421 cggtaatgta taaaaacaga ttaagttttg ccggagaggg ccactgtgtc cgcattattt
     6481 cactatccag ttatccattt ttaacttgat gcgttgacca ccgtcggtga gttccatatt
     6541 ggctggcatc gcaggttgag tttttgtgtc ataaccaccg taaacaactt tccagttttt
     6601 gccattctgg ctgtaggtga tttcgctcag gcggtactgg tcgtccagtt tgtagtcggt
     6661 tgcatcaccc ggtaaaccta aaatccactg gcgcaagctg ttgagcggaa ttggcattcc
     6721 ggtcaatttg ccaatcatct cttcggcgtc atcggcggta taacgctggc ctttattgtc
     6781 gactaactgc acgttacccg gttgagcatt cagctccagt tccgtgctgc ctaatgggtt
     6841 agtcagcagc agacggtagc gatcctggcc agtttgctgc cagaagaaac gggcgtacac
     6901 tttttgttgg tcagaaatat aagcgaacgc gccgcgagtt tgatactgat taagattgcg
     6961 cacgtcttgc tgatgctgac gccattgtgg tgaatccggg cttttgccag gacctttggg
     7021 tgtggtaacg gaacaggcag tgagcacaag agcagccagc ggtagcaggc ggataagacg
     7081 aaaatcgggc aggggcatag tgatgacaag tccttgagat acgttgcagt tataaccctt
     7141 aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt gtcttaattg ccagaatcta
     7201 acggctttcg gcaattactc caaaaggggg cgctctcttt tattgatctt acgcatcctg
     7261 tatgatgcaa gcagactaac cctatcaacg ttggtattat ttcccgcaga catgaccctt
     7321 ttagcactcg gtatcaacca taaaacggca cctgtatcgc tgcgagaacg tgtatcgttt
     7381 tcgccggata agctcgatca ggcgcttgac agcctgcttg cgcagccgat ggtgcagggc
     7441 ggcgtggtgc tgtcgacgtg caatcgcacg gaactttatc ttagcgttga agagcaggat
     7501 aacctgcaag aggcgttaat ccgctggcta tgcgattatc ataatcttaa tgaagaagat
     7561 ctgcgtaaaa gcctctactg gcatcaggat aacgacgcgg ttagccattt aatgcgtgtt
     7621 gccagcggcc tggattcact ggttctgggg gagccgcaga tcctcggtca ggtaaaaaaa
     7681 gcctttgctg attcgcaaaa aggtcatatg aaggccagcg aactggaacg catgttccag
     7741 aaatctttct ctgtagcgaa acgcgttcgc actgaaacag atatcggtgc cagtgctgtg
     7801 tctgtcgctt ttgcggcttg tacgctggcg cggcagatct ttgaatcgct ctctacggtg
     7861 acggtactgc tggtgggtgc gggcgaaacc atcgagctgg tggcgcgtca tctgcgcgaa
     7921 cataaagtac agaagatgat tatcgccaac cgcactcgcg aacgtgccca aattctggca
     7981 gatgaagtcg gcgcggaagt tattgccctg agtgatatcg acgaacgtct gcgcgaagcc
     8041 gatatcatca tcagttccac cgccagcccg ttaccgatta tcgggaaagg catggtggag
     8101 cgcgcattaa aaagccgtcg caaccaaccg atgctgctgg tggatattgc cgttccgcgc
     8161 gatgttgagc cggaagttgg caaactggcg aatgcttatc tttatagcgt ggacgatctg
     8221 caaagcatca tttcgcacaa cctggcgcag cgtaaagccg cagcggttga ggcggaaact
     8281 attgtcgctc aggaaaccag cgaatttatg gcgtggctgc gtgcacaaag cgccagcgaa
     8341 accattcgcg agtatcgcag ccaggcagag caagttcgcg atgagttaac cgccaaagcg
     8401 ttagcggccc ttgagcaggg cggcgacgca caagccatta tgcaggatct ggcatggaaa
     8461 ctgactaacc gcttgatcca tgcgccaacg aaatcacttc aacaggccgc ccgtgacggg
     8521 gataacgaac gcctgaatat tctgcgcgac agcctcgggc tggagtagca gtacatcatt
     8581 ttcttttttt acagggtgca tttacgccta tgaagccttc tatcgttgcc aaactggaag
     8641 ccctgcatga acgccatgaa gaagttcagg cgttgctggg tgacgcgcaa actatcgccg
     8701 accaggaacg ttttcgcgca ttatcacgcg aatatgcgca gttaagtgat gtttcgcgct
     8761 gttttaccga ctggcaacag gttcaggaag atatcgaaac cgcacagatg atgctcgacg
     8821 atcctgaaat gcgtgagatg gcgcaggatg aactgcgcga agctaaagaa aaaagcgagc
     8881 aactggaaca gcaattacag gttctgttac taccaaaaga tcctgatgac gaacgtaacg
     8941 ccttcctcga agtccgtgcc ggaaccggcg gcgacgaagc ggcgctcttc gctggcgatc
     9001 tgttccgtat gtacagccgt tacgccgaag cccgccgctg gcgtgtagaa atcatgagcg
     9061 ccagcgaggg tgaacatggt ggttataaag agatcatcgc caaaattagc ggtgatgttg
     9121 tatatggtcg tctgaaattt gaatccggcg gtcatcgcgt gcagcgtgtt cctgctacgg
     9181 aatcgcaggg tcgtattcat acttctgctt gtaccgttgc ggtaatgcca gaactgcctg
     9241 acgcagaact gccggacatc aacccagcag atttgcgcat tgatactttc cgttcgtcag
     9301 gggcaggtgg tcagcacgtt aacaccaccg attcggcaat tcgtattacc cacttgccga
     9361 ccgggattgt tgttgaatgt caggacgaac gttcacagca taaaaacaaa gctaaagcac
     9421 tttctgtgct tggtgctcgc atccacgctg cagaaatggc aaaacggcaa caggccgaag
     9481 cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag cgaccgtaac cgtacttaca
     9541 atttcccgca ggggcgcgtt accgatcacc gcatcaacct gacgctctac cgtctggatg
     9601 aagtgatgga aggtaagctg gatatgctga ttgaaccgat tatccaggaa catcaggccg
     9661 accaactggc ggcgttgtcc gagcaggaat aatggaatat caatactggt tacgtgaagc
     9721 aataagccaa cttcaggcga gcgaaagccc gcggcgtgat gctgaaatcc tgctggagca
     9781 tgttaccggc agagggcgta cttttattct cgcctttggt gaaacgcagc tgactgacga
     9841 acaatgtcag caacttgatg cgctactgac acgtcgtcgc gatggtgaac ccattgctca
     9901 tttaaccggg gtgcgagaat tctggtcgtt gccgttattt gtttcgccag cgaccttaat
     9961 tccgcgcccg gatacggagt gtctggtgga gcaggcactg gcgcggttgc ctgaacaacc
    10021 ttgccgtatt ctcgatctcg ggacgggtac cggggcgatt gcgctggcgc tggctagcga
    10081 gcgcccggac tgcgaaatta ccgctgtaga tcgtatgccc gatgctgtct ctctggcgca
    10141 acgtaatgcc cagaatctgg cgatcaaaaa catccatatt ctgcaaagcg actggtttag
    10201 cgcgctagcc gggcagcagt ttgcgatgat tgtcagtaat ccgccgtata ttgacgagca
    10261 ggacccacat cttcaacaag gcgatgttcg ctttgagccg ctcactgcgc tggttgcggc
    10321 agacagtgga atggcagaca tcgtgcatat catcgaacag tcgcgtaacg cgctggtatc
    10381 cggcggcttt ctgcttctgg aacatggttg gcagcagggc gaagcggtgc gacaggcatt
    10441 tatcctcgcg gggtatcatg acgtcgaaac ctgtcgggac tatggtgata acgagcgcgt
    10501 aacgctcggc cgctattatc aatgacaagt ttttctacac tgcttagcgt tcatctcatt
    10561 agcattgcgc tttctgtagg gctattaacc ttacgtttct ggctacgtta tcagaagcat
    10621 ccacaggcat ttgcgcgctg gacgcgcatt gtgccgccgg ttgtcgatac ggtgttattg
    10681 ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc cattttccgg gcaggcacag
    10741 tggctgactg aaaagctgtt tggagttatc atttatatcg ttttgggttt tattgcattc
    10801 gattatcgtc gtatgcacag tcagcaggcg cgcattattg ccttcccgct ggcgttggtg
    10861 gtgctgtaca tcatcattaa actcgccacc acaaaagtac cgttactggg gtaagtcatg
    10921 agatcgttag ctgatttcga atttaataaa gcgccattgt gcgaaggcat gatcctggct
    10981 tgcgaagcaa tccgccgcga ttttccctcg caagatgttt acgacgaact ggagcgtctc
    11041 gttagtctgg cgaaggaaga aatcagccag cttctgcctt tagaagagca gttggaaaaa
    11101 ctgatcgcgc tgttttacgg cgactgggga tttaaagcct cacgcggcgt ttatcgcctt
    11161 tccgatgcat tatggctgga ccaggtgtta aagaatcgac agggcagtgc ggtatcatta
    11221 ggtgccgttt tattatgggt cgcgaatcgt ctcgatttgc cgctattgcc ggtgattttc
    11281 ccgacgcagc tgatattgcg cattgaatgt ccggatggcg aaatttggct gattaatcct
    11341 tttaacggtg aatcgttaag cgaacatatg ctggatgtat ggttaaaggg aaatatcagc
    11401 ccgtcggcgg aactgtttta tgaagacctt gatgaagctg ataacattga ggtaatccgc
    11461 aaattgctgg atacactcaa agcttcgttg atggaagaaa atcagatgga gctggcgtta
    11521 cgcaccagcg aagctttatt acaattcaac ccggaagatc cctatgaaat tcgcgatcgc
    11581 gggttgattt atgcgcaact ggattgcgaa cacgttgcgt tgaacgattt aagttatttc
    11641 gttgaacagt gtccggaaga cccgatcagc gaaatgatcc gtgcgcaaat aaataacatc
    11701 gcgcataaac atattgtgct gcattaatta atcgacattt tactcaagat taaggcgatc
    11761 ctatgaaaca aaaagtggtt agcattggcg acatcaacgt agcaaatgac ctgccgttcg
    11821 tactgtttgg cggtatgaac gtgctagaat ctcgcgatct ggcgatgcgc atttgcgagc
    11881 actacgtaac cgtgacccag aaactgggta tcccttacgt gttcaaagcc tcttttgaca
    11941 aagccaaccg ctcctccatc cactcttatc gtggaccggg cctggaagaa gggatgaaaa
    12001 tcttccagga gttgaagcag acttttggcg tgaaaattat caccgacgtt cacgaaccaa
    12061 gccaggctca gcccgttgct gatgttgtgg atgtgattca gttgccggcg tttcttgctc
    12121 gccagactga cctggttgaa gccatggcga aaaccggtgc ggtaattaac gtcaagaaac
    12181 cgcagtttgt cagcccggga cagatgggta atatcgttga taaattcaaa gaaggcggca
    12241 acgaaaaagt gattctttgc gatcgcggtg ctaacttcgg ctatgacaac ctggttgttg
    12301 atatgctggg cttcagcatc atgaagaaag tgtctggtaa ttcaccggtg attttcgacg
    12361 tgacccacgc actgcaatgc cgcgatccgt ttggcgcagc ttccggcggt cgtcgtgctc
    12421 aggtggctga gctggcacgt gccggtatgg cggtaggtct ggcggggctg tttattgaag
    12481 cgcatccgga tccggaacat gcgaaatgtg atggtccatc cgcgctgccg ctggctaaac
    12541 tggaaccgtt cctcaagcag atgaaagcga ttgatgatct ggtgaaaggt ttcgaagaac
    12601 tggataccag caagtaatct tttttgcttg aaaactaaag ccttagcgtt ctgcgttaag
    12661 g
//
LOCUS       DARNVM010000143        12624 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.143, whole genome shotgun sequence.
ACCESSION   DARNVM010000143 DARNVM010000000
VERSION     DARNVM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.143"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..109
                     /locus_tag="SI621_003654"
     CDS             <1..109
                     /locus_tag="SI621_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066318.1"
                     /translation="SVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            223..861
                     /locus_tag="SI621_003655"
     CDS             223..861
                     /locus_tag="SI621_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HEI4066319.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     DVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEDVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            858..1595
                     /locus_tag="SI621_003656"
     CDS             858..1595
                     /locus_tag="SI621_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HEI4066320.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSSADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSKTPDALNADILQTLTQRIPQ"
     gene            1595..3691
                     /locus_tag="SI621_003657"
     CDS             1595..3691
                     /locus_tag="SI621_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEI4066321.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3738..4016)
                     /locus_tag="SI621_003658"
     CDS             complement(3738..4016)
                     /locus_tag="SI621_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066322.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKTDLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4288..4698
                     /gene="psiE"
                     /locus_tag="SI621_003659"
     CDS             4288..4698
                     /gene="psiE"
                     /locus_tag="SI621_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HEI4066323.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4792..5682)
                     /gene="malG"
                     /locus_tag="SI621_003660"
     CDS             complement(4792..5682)
                     /gene="malG"
                     /locus_tag="SI621_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEI4066324.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5697..7241)
                     /gene="malF"
                     /locus_tag="SI621_003661"
     CDS             complement(5697..7241)
                     /gene="malF"
                     /locus_tag="SI621_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEI4066325.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSTLFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7395..8585)
                     /gene="malE"
                     /locus_tag="SI621_003662"
     CDS             complement(7395..8585)
                     /gene="malE"
                     /locus_tag="SI621_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HEI4066326.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGHPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            8950..10065
                     /gene="malK"
                     /locus_tag="SI621_003663"
     CDS             8950..10065
                     /gene="malK"
                     /locus_tag="SI621_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HEI4066327.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10137..11477
                     /gene="lamB"
                     /locus_tag="SI621_003664"
     CDS             10137..11477
                     /gene="lamB"
                     /locus_tag="SI621_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HEI4066328.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11629..12549
                     /gene="malM"
                     /locus_tag="SI621_003665"
     CDS             11629..12549
                     /gene="malM"
                     /locus_tag="SI621_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HEI4066329.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3034 a         3319 c         3381 g         2890 t
ORIGIN      
        1 cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga
       61 tggttctaat accgggacca caacaaccac gaccaccagt acccagtaat aaagtatgta
      121 tccccaaaat aattcgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa
      181 ccacactccg gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc
      241 attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattcg
      301 ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat
      361 atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg
      421 gcctttgctg aagacggaca acaaaaatgg gtcactcagg atcaggccac tctcgtcaca
      481 caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga cgtgaataac
      541 cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc
      601 acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa
      661 tgggatggca ccgataccgt caaagtcggc agcgatgaaa ctccggttcg cgtgctggac
      721 gaagacgtct ccaccgacca ggcgcgctgg cataaccgct attggatcga cagcgaaggg
      781 caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc
      841 atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc attgattttg agtgtggggg
      901 cgtcatcggt cttcgcggcg ggaaccgtca aggtattcag caatggcagc agtgaggcca
      961 aaacgctgac gggggcagag catttaatcg atctggtagg ccaaccgcgg ctggcaaaca
     1021 gttggtggcc cggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc
     1081 agcaggcgtt gctgacacgg ctggcagaac tagccgcaga ttccagcgct gacgatgccg
     1141 ccgccattaa cgccttacgc cagcaaattc aggcgttgaa ggtgacgggc aggcaaaaaa
     1201 tcaatcttga tcccgatatc gtccgcgttg ctgaacgcgg taacccgccg ttgcagggca
     1261 actacacgct gtgggtcgga ccaccgccgt ccacggtcac gttattcggg cttatcagcc
     1321 gtcctggcaa gcagccattc actccaggtc gcgacgtcgc aagctatctc tccggccaga
     1381 acctgcttag cagtgcggat cgcagctacg cgtgggtggt ttacccggac ggacgcacgc
     1441 aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta
     1501 tttatgttgg cctcgcggac tccgtctgga gtaagacgcc tgatgccctg aacgccgaca
     1561 ttcttcagac tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt
     1621 actggcgctg ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc
     1681 gtcgcagtcg gatttcggtg gcgtaggatt attacaaacg cccaccgcac gtatggcacg
     1741 ggaaggggag ctgagcctga actatcgcga taacgatcag taccgttatt actcagcttc
     1801 agtgcaactc ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca
     1861 gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct
     1921 caaactgcgt ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga
     1981 tatcggcggt acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc
     2041 gttcgatttt acgctcggcc tgggatgggg atatctgggc accagcggta atgtgaaaaa
     2101 tccgctctgt tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg
     2161 atctatcgac ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata
     2221 ccagacgccc tggcaaccgc tgcgcctgaa actggagtat gaaggcaata attatcagca
     2281 ggattttgcc gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt
     2341 taccgattgg gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt
     2401 cacgctgcgc accaacttta acgatctgcg cccgtcctac aacgataacg cccgcccgca
     2461 atatcaaccg cagccgcagg atgccatttt gcagcattcg gtggtggcaa atcagttaac
     2521 gctgttgaaa tacaacgccg gactggccga tccgcagatc caggcgaaag gcgatacgct
     2581 gtatgtcacc ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa
     2641 tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggagaatcg
     2701 ccttaacatg ccgcaggtga cgacggaaac cgatgtcgcc agcctgaaaa atcatcttgc
     2761 cggagagccg ttgggccacg aaacgacgct ggcgcaaaaa cgcgtcgagc cagtggttcc
     2821 gaagtccacc gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc
     2881 ggtgctgaac cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat
     2941 gggaacggca gatttgtggc tgacggacca tctgctgacc actggaagcc tgtttgcaaa
     3001 tcttgccaac aactacgaca agtttaacta cactaatcca ccgcaggact cgcacttacc
     3061 gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca
     3121 agccaactac ttccagcatc tgggcaacgg cttctacggt caggtctacg gtggttatct
     3181 cgaaaccatg tttggcggtg cgggggcaga agtgttgtat cgcccgctgg acagcaactg
     3241 ggcgtttggt ctggatgcca actacgttaa acagcgcgac tggcgtagcg caaaagatat
     3301 gatgaaattc accgactaca gcgtgaaaac cggacatctg accgcatact ggacgccatc
     3361 tttcgctcag gatgtgttag tgaaagcgag cgtcgggcag tatctggcag gggataaagg
     3421 cggcacgctg gagatcgcca aacgctttga tagcggcgtg gtggtgggtg gctatgccac
     3481 gatcaccaat gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt
     3541 ctctgtaccg ctggatctct tctcgtctgg cccgacacgc agccgtgcgg caattggctg
     3601 gacgccgctg acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat
     3661 gaccagcgac cgtagcgtca atttccgcta agtcatggga aaggtgccag ttttcgcact
     3721 cactggcacc tttattctta atggcctgtc gagccatcag gaacgcagtt cacccccggt
     3781 ttgcgatctg gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tattggttgc
     3841 aggtctggtt gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg
     3901 tcggttttat cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac
     3961 atcaaagcag caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt
     4021 tccagaggaa cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg
     4081 tgatttttgc agtaccggct atggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg
     4141 cttatccagc ctacgggtag gccgcatccg gcgttgatgg gagatgctct ctaaatccag
     4201 gttgaacaaa acatacacaa aaaatataga tcaccgtcac atttttgcgt tatacaggaa
     4261 gctcgccatt gtgaaggagg tactgctatg acgtcactct ctcgtccgcg cgtggagttt
     4321 atctccacca ttttgcagac cgtactcaat cttggcctgc tgtgcctcgg cctgattttg
     4381 gttgtcttcc tcggcaaaga aacggtgcat ctggctgatg tgctgttcgc gccagaacaa
     4441 accagcaaat atgagctggt agaagggctg gtggtttact ttctctattt cgaatttatc
     4501 gcgctgattg tgaagtactt tcagtccggt tttcacttcc cgctacgtta ctttgtctat
     4561 attgggatca ccgccattgt gcgcttgatc atcgtcgatc ataaatcgcc actggatgtg
     4621 ttgatctact cggcagcgat cctgttgctg gtgatcaccc tgtggttatg taattcgaaa
     4681 cggttgaagc gggagtaaaa aaaggcgctc cgtggagcgc cgaataacag tcacaagttg
     4741 ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc tttaaccttt
     4801 cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcaaga agacgatggt
     4861 gatcggtaat gcagacatca ctgcagcggc ggcaaagtca ccccacaggt agttttgcgg
     4921 gttgaggtat tgctgcattc ccacggccag ggtgtagctg tttacgtcac gcagtaacag
     4981 cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca ccaccgccag
     5041 aatcggtact gacagcggta acaagacaag gcggaaggct tgccacggtg tcgcaccatc
     5101 cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt tgatggtcca
     5161 gacatgcagc gcaatcccac ccagatacgc gaaaattacg ccgccgtgag tattcaggcc
     5221 aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga ccagtgaaag
     5281 tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa
     5341 gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc caatcgcgga
     5401 aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg gcggtggcgt
     5461 aatgcgacca tcagcctgtt ccacactaaa acctaacgcc agtttccagt gatcccagga
     5521 gatttgctcc gggatcagac taccggtcgc gaagttcccc tgacgcagtg agatagcgac
     5581 gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca ggtgagtaat
     5641 aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta tctcccttaa
     5701 tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcgcccac cagcaggaag
     5761 atcagcgtgg caattgctgc tgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg
     5821 atgcggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga
     5881 cgatccgggc cgccgttggt taacagttga atcagcacga agttgttaaa gttaaaggca
     5941 aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt aatcttaaag
     6001 aagttctgga atggacctgc gccatccatt gctgaggctt catacaaatc gtccggaatc
     6061 gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca ggtgttgacg
     6121 ataatcagca tcgtgcgggc ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac
     6181 agcgtgctca acatcatgtt gatctcaccg aagctctggt taaacaaccc tttgaaaatc
     6241 aagattgaaa tgaacgatgg caccgcatag ggcagaatca gcaggacgcg atagaccgct
     6301 tttccgcgca acgcttccca ctgcaccaga cacgccagaa ccatgccgac cgccaccgtt
     6361 aaaaagacgg tgatcagcga gaacaccacg gtccagacga aaatggcgag gaacggtttc
     6421 tgaatgcctt cgtcggtgaa gacgcgggta aagtttttcc agccagtggt cacggtgtaa
     6481 ccggggctta gcttttcatc accccagttg ccgtcggcgg taatggactg gtaaaagcca
     6541 atttggttat tcggacgata tttcacgccg ctctgattat tcgtcaacgt gccgtcaccg
     6601 tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat catcactttg
     6661 ttgccatctg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg ggtaatcacg
     6721 cgcagattcg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg cagtttttgc
     6781 tcgccgccaa atttaaacgc gtcggagagg taatttttgc cggtttcgcc gtcgctgagc
     6841 gccagttgcc actcatcgcc cgccgggtaa agaccaaaat tataggtttt gcctgcttgc
     6901 caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt agtgctgctg
     6961 tagttggtga aggcaatggc gatggtgcag accaaaggga agaggacgaa taatcccatt
     7021 ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc gaaaatatac
     7081 agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc ttgtgcgtac
     7141 attaaaacaa caaggtaacc caccagcagg ccgagcagac ctaacactga ccatttcagc
     7201 gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt cctcattcca
     7261 ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac gctgacgcgc
     7321 cttaccaggc ctacatacgt ttcaatttcg taggccggat aaggcgttca cgccgcatcc
     7381 ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc ttcatcgaca
     7441 gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac
     7501 atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt ggcggcaata
     7561 cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg
     7621 tctttattaa ccgcttccag accttcgtca gtcagcagat agttttcgag gaactctttc
     7681 gccagctctt tgttcggact ggcggcgtta atacctgcgc tcagcacgcc aacgaacggt
     7741 ttggatggat gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg
     7801 tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc tttattaaag
     7861 gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt aatcaggtca
     7921 accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc tttaatgtcg
     7981 tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag cggccaggtg
     8041 aagtacggtt cttgcaggtt gaacatcagc gcgctcttgc ctttcgcttt cagttcttta
     8101 tccagcgccg ggatctcttc ccaggttttt ggcgggttcg gcagcagatc tttgttataa
     8161 atcagcgata acgcttcaac agcgatcggg taagcaatca gcttgccgtt gtaacgtacg
     8221 gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt gatttcagcc
     8281 aacaggccag attgagcgta gccaccaaag cggtcgtgcg cccagaagat aatgtcaggg
     8341 ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg atgctcaacg
     8401 gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag accgttatag
     8461 cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc gagagccgag
     8521 gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc tgtttttatt
     8581 ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac ggactctagt
     8641 ttctttatac agcaacctct ttccatcctc cttgccccta cgccccaccg ttgctttgtg
     8701 tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc gccacgattt
     8761 ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag aagccgcgtt
     8821 ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt aagccatctc
     8881 ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt gttacaaagg
     8941 gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg cgaggtcgtg
     9001 gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt tgtcggaccg
     9061 tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac gatcaccagc
     9121 ggcgacctgt tcatcggtga gaaacggatg aatgacactc cgccagcaga acgcggcgtt
     9181 ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga aaacatgtca
     9241 tttggcctga aactggcagg cgcaaaaaaa gaggtgatta accaacgcgt caaccaggtg
     9301 gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct ctccggtggt
     9361 cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg agccaagcgt atttttgctc
     9421 gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat cgaaatctcc
     9481 cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca ggtcgaagcg
     9541 atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca ggttgggaaa
     9601 ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat cggttcgcca
     9661 aagatgaact tcctgccggt aaaagtgacc gccactgcaa tcgatcaagt gcaggtggag
     9721 ctgccgatgc caaatcgtca gcaagtctgg ctgccggttg aaagccgtga tgtccaggtt
     9781 ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccaagtga tatcgctgac
     9841 gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac ccaaatccat
     9901 atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt ggtgttggta
     9961 gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct gttccgtgag
    10021 gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc ccacaaaaca
    10081 cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga gatagaatga
    10141 tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta atgtctgctc
    10201 aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg acaggtagcg
    10261 gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt cttggcaacg
    10321 aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagaa ggcgataaga
    10381 gcttctattt cgacactaac gtggcctatt ccgtcgcaca acagaatgac tgggaagcta
    10441 ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa tggctgccag
    10501 gctccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat atgatcgact
    10561 tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat gttggcttcg
    10621 gtaaactctc tctggcagca acccgctcct ctgaagctgg tggttcttcc tctttcgcca
    10681 gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgac gtgcgtttag
    10741 cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac ggtcgtgcca
    10801 acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggttgg ttattcactg
    10861 ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag tacgctactg
    10921 actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg tttgataacg
    10981 aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg
    11041 cgatctccat gggcgataac tgggacatga tgtacgtggg tatgtaccag gatatcaact
    11101 gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg tacaagtgga
    11161 cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg
    11221 gcgacaagaa caatcagtac aaaattaccc ttgcacaaca atggcaggct ggcgacagca
    11281 tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg
    11341 gttatgacta caccggtaac gccaatacca acactaactt cggcaaagcc gttcctgctg
    11401 atttcaacgg cggcagcttc ggccgtggtg acagcgacga gtggaccttc ggtgcccaga
    11461 tggaaatctg gtggtaatag caaaaactgg gccggataag gtgcttgcgc cgcatccggc
    11521 ataaaaacag gttgtcatta tctgaaaggg gcgcaagccc ctctgattat cgggtttagc
    11581 gcgctattgc ctggctaccg ctgagctcca gattttgagg tgaaaacaat gaaaatgaat
    11641 aaaagtctca tcgccctctg tttatcagca gggttgctgg caagcgcgcc tggaatcagc
    11701 cttgccgatg ttaactacgt accgcaaaac accagcgacg cgccagccat tccatctgcc
    11761 gcgctgcaac aactcacctg gacaccggtc gatcaatcta aaacccagac cacccaactg
    11821 gcgactggcg gccaacaact gaacgttccc ggcatcagtg gtccggttgc tgcgtacagc
    11881 gtcccggcaa acattggcga actgacccta acgctgacca gcgaagtgaa caaacaaacc
    11941 agcgtatttg cgccgaacgt gctgattctt gatcagaata tgaccccatc agccttcttc
    12001 cccagcagtt atttcaccta ccaggaacca ggcgtgatga gtgcagatcg tctggaaggc
    12061 gttatgcgcc tgacaccggc gttggggcag caaaaacttt atgttctggt ctttaccacg
    12121 gaaaaagacc tccagcagac gacccaactg ctcgacccgg ctaaagccta tgccaaaggc
    12181 gtcggtaact cgatcccgga tattcctgac ccggttgctc gtcataccac cgatggctta
    12241 ctgaaactga aagtcaaaac gaactccagt tccagcgtgt tggtcgggcc actatttggt
    12301 tcttccgctc cagctccggt tacggtaggc aacacggcgg caccagctgt ggctgcaccc
    12361 gctccggcac cggtgaagaa aagcgagccg atgctcaacg acacggaaag ttattttaat
    12421 accgcgatca aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa actgcttgat
    12481 gaagctgaac gcctggggtc aacatctgcc cgttccacct ttatcagcag tgtaaaaggc
    12541 aaggggtaat tacgccccac agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac
    12601 ctttttttat gcttcattcc aggg
//
LOCUS       DARNVM010000144        12618 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.144, whole genome shotgun sequence.
ACCESSION   DARNVM010000144 DARNVM010000000
VERSION     DARNVM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.144"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..574
                     /locus_tag="SI621_003666"
     CDS             <1..574
                     /locus_tag="SI621_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066330.1"
                     /translation="NGISKPTDLFTWHGKDKKYNTLAIVIKNTNGNDYLSLGYYDQDN
                     YHIKRGIRINGDSLTQYCSENARNASASFESNKALMAELFATGINHQVVNELNGERLK
                     EPNEVFKRYGRAIRYNFQVDNAKYRRNNVKEIVSTLFDNEVNTDHSQNIYVHYKKLEG
                     KIEERLQNCQTEYQNEINQLSALGVNFDDV"
     gene            complement(756..1511)
                     /gene="mlaA"
                     /locus_tag="SI621_003667"
     CDS             complement(756..1511)
                     /gene="mlaA"
                     /locus_tag="SI621_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HEI4066331.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1805..2737
                     /locus_tag="SI621_003668"
     CDS             1805..2737
                     /locus_tag="SI621_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEI4066332.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2813..2887
                     /locus_tag="SI621_003669"
     tRNA            2813..2887
                     /locus_tag="SI621_003669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2846..2848,aa:Arg,seq:cct)
     gene            3097..3249
                     /locus_tag="SI621_003670"
     CDS             3097..3249
                     /locus_tag="SI621_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066333.1"
                     /translation="MHSLMNSQARDTRDRSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(3329..4576)
                     /locus_tag="SI621_003671"
     CDS             complement(3329..4576)
                     /locus_tag="SI621_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066334.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4638..5561)
                     /locus_tag="SI621_003672"
     CDS             complement(4638..5561)
                     /locus_tag="SI621_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HEI4066335.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHAEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELESEK"
     gene            5777..7210
                     /gene="cscA"
                     /locus_tag="SI621_003673"
     CDS             5777..7210
                     /gene="cscA"
                     /locus_tag="SI621_003673"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEI4066336.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(7218..8276)
                     /locus_tag="SI621_003674"
     CDS             complement(7218..8276)
                     /locus_tag="SI621_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEI4066337.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPKDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            8456..8674
                     /locus_tag="SI621_003675"
                     /pseudo
     CDS             8456..8674
                     /locus_tag="SI621_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8692..10020
                     /gene="dsdA"
                     /locus_tag="SI621_003676"
     CDS             8692..10020
                     /gene="dsdA"
                     /locus_tag="SI621_003676"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HEI4066338.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRNAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(10128..11666)
                     /gene="emrY"
                     /locus_tag="SI621_003677"
     CDS             complement(10128..11666)
                     /gene="emrY"
                     /locus_tag="SI621_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HEI4066339.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVMSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11666..>12618)
                     /gene="emrK"
                     /locus_tag="SI621_003678"
     CDS             complement(11666..>12618)
                     /gene="emrK"
                     /locus_tag="SI621_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HEI4066340.1"
                     /translation="DTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQD
                     KQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQA
                     YKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVS
                     PGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGT
                     GNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAE
                     MPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3583 a         3015 c         2877 g         3143 t
ORIGIN      
        1 gaacgggata agcaaaccaa cggacttatt tacctggcac ggtaaagata aaaaatacaa
       61 cactctcgcc attgtaatta aaaatacaaa cggaaacgat tatttatctc tcggttacta
      121 cgatcaggac aactaccata ttaaaagagg aattcgtatt aatggtgata gtctcacaca
      181 atattgtagt gaaaacgcca ggaatgcttc agcgtcattt gaaagcaata aagctctcat
      241 ggcagaatta tttgcaacag gtataaatca tcaagttgta aacgaactca acggggaaag
      301 actgaaagaa ccaaacgaag tttttaaacg ttatggtcga gcaataagat ataattttca
      361 agtggacaat gcaaaatata gacgcaataa tgtaaaagaa atagtttcta ctttattcga
      421 taacgaagtt aacactgacc attctcaaaa tatatatgtg cactataaga aactcgaagg
      481 taaaattgaa gaaaggcttc aaaattgcca gacggaatat caaaatgaaa ttaatcaact
      541 atctgcgtta ggtgttaatt ttgatgatgt ttaaaattat ttttccagtt ttttgatttt
      601 tcaacgactg aatatctgaa atagtcaatg acgaacatca gtatttttac atcattacgt
      661 ccgggtcatt gtcggtttat ctccttttac ttgtggtaat tgagggataa caacaaaaaa
      721 ggtgagtttt gcgactcacc ttttttattt gtttcttatt cagaatcgat atcttttaaa
      781 tcatcctgaa tcgcttgcgc gttcggattt tcctgcggtt tgagttcgcc gccattagcg
      841 atgaaatcat gacgctggaa gtacgcttcg cgcaccataa tgtaaggatc ggacgactga
      901 cgcagcagac cgtcggaatc cagcagctgt gcgcgggttt cgataccttc aagcgtccat
      961 ttacccacag acatcggcca ggtcaaccag gaaagaaccg ggtaaagacc atccgccata
     1021 tcaccaccgt catcacgcag cgtgaagcta ccgtagaacg gtaactgaac gtatggccca
     1081 taacccacac cataatgacc aagcgtacta ccgaagcggt gaggttcagt ccgttgcagt
     1141 ttcgggttcg ccatccctgc aacatcaata aagccgccca tccccaaaat ggtgttcagg
     1201 aaaaagcggg taaagtggac catcccctga taagggtcgc cctgcaagaa gtagttaacc
     1261 atcaccgcag gttcttcaag gttgccagta aagttgctca aaccgttacg cgccggttgc
     1321 ggaacataat cacgccaggc gacagcgact ggtcgaacaa tatacgggtc taatacattg
     1381 aagttgaagt tgtacatggt gcggttgaac ccttctaacg ggtcagaacg cccttgctga
     1441 tctgtaccgg aactcgcaca ccccaccaga agcgtagttc ccagagcaag cgccgacagg
     1501 cgaagcttca taaatgtctc cctgtttttt tatggcttat gcagtttgcc atccatgacg
     1561 gaacgatacc gtatccgcct gtttaggtgt gggcgattgt aacagcacgt caactgatgt
     1621 ccagacgccc tgatttgctg atttgatcat agcctggtaa tcgccgccct gtaggctact
     1681 tgattctata gaaacagaaa aaggcaaacg cggccttttc tacattttca gagtaactcc
     1741 cgccagttgc gagcaaaaaa gccgctacgc tttagctata cgtgctaatt caagagaaga
     1801 gaccatggac aacgacaaaa ttgatcaaca cagcgacgaa attgaagttg agagcgaaga
     1861 aaaagagcgc ggcaaaaaaa tagaaataga tgaagatcga ctcccctccc gggcgatggc
     1921 aattcatgag catatccgcc aggatggtga aaaagagctg gaacgcgacg caatggcact
     1981 actgtggtca gccattgcgg cgggtctgtc gatgggcgct tcattactgg caaaagggat
     2041 atttcatgtc gaactggaag gcgtgccggg cagcttcttg ctggagaatc tcggttatac
     2101 ctttggtttt attatcgtca ttatggcccg ccagcaatta tttaccgaaa acaccgtgac
     2161 tgcggtacta cccgtcatgc aaaaaccgac aatgagcaac gttggcttac ttatgcggtt
     2221 atggggcgtc gtgctgctgg gtaatattct cgggacaggt attgcggcgt gggcatttga
     2281 atatatgcca atcttcaatg aagaaactcg cgatgcattt gtcaaaatcg gcatggatgt
     2341 gatgaagaac acccccagcg agatgtttgc caacgcgatc atttccggct ggctgatcgc
     2401 cactatggta tggatgtttc ctgcagcggg tgcggcaaag attgtggtga ttatattgat
     2461 gacctggctt attgccctgg gtgacaccac tcacatcgtt gtcggttctg ttgaaatcct
     2521 ctatctggtg tttaacggta cgctgcactg gagcgatttc atctggccct tcgcactacc
     2581 tactttagcg gggaacatct gcggcggcac ctttatcttc gcgttaatga gtcatgcaca
     2641 gatccgcaac gacatgagca acaagcgcaa agcagaagca cgccaaaaag cagaacgtgc
     2701 ggaaaacatt aagaaaaatg ataaaaaccc ggcataaatg gcgagggttt aagcaatcga
     2761 gcggcagcgt acttaccccg cagtccatta gcgggtatac tcatgccgca ttgtcctctt
     2821 agttaaatgg atataacgag cccctcctaa gggctaattg caggttcgat tcctgcaggg
     2881 gacaccagat acccttcaaa ccaaatctac cttcaccccg taaaagatgg gtttggcagc
     2941 acacttgccc tatatctact catttttact gcaacaggtt gaaatctcag cactgtcaga
     3001 aagcgctgat gactaaacag ccctgggccg ggcgatgtaa ccatcataca gaatcctgat
     3061 agcgaaatat ggcgtgactc gatacttcac tctgcaatgc attccttgat gaattcgcag
     3121 gcccgtgata ctcgggacag gtcgctgaat gacgacaatg tcctggaaat cagcgaaccg
     3181 cgtatccgga gtacatttga gcgactgtac cagaacatga atgaggcgtt tggattaggt
     3241 gattattagt tgggctaagc atttttgtat tattattttc cggttgaggg atatggagat
     3301 atcgacaaca accggaaaaa gtttacgtct atattgctga aggtacaggc gtttccataa
     3361 ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa tagcaacatc aggcagacaa
     3421 tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg gtcaaagagt atcccagtcg
     3481 gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg aaagccaatc agaaagatcg
     3541 tcgacgacag gcgcttatca aagtttgcca cgctgtattt gaagacggat atgacacaaa
     3601 gtggaacctc aatggcatgt aacagcttca ctaatgaaat aatccagggg ttaacgaaca
     3661 gcgcgcagga aaggatacgc aacgccataa tcacaacacc gataagtaat gcattttttg
     3721 gccctacccg attcacaaag aaaggaatga tcgccatgca cagcgcttcg agtaccacct
     3781 ggaatgagtt gagataacca tacaggcgcg ttcctacatc gtgtgattcg aataaacctg
     3841 cataaaagac aggaaagagt tgttgatcaa aaatgttata gaaagaccac gtccccacaa
     3901 taaatatgac aaaaacccag aagtttcgat ccttgaaaac tgcgataaaa tcctcttttt
     3961 ttacccctcc cgcatccgcc gctatgcact ggtgatcctt atctttaaaa cgcatgttga
     4021 tcatcataaa tacagcgcca aatagcgaga ccaaccagaa gttgatatgg ggactgatac
     4081 taaaaaatat gccggcaaag aacgcgccaa tagcatagcc aaaagatccc caggcgcgcg
     4141 ctgttccata ttcgaaatga aaatttcgcg ccattttttc agtgaagctg tcaagcaacc
     4201 cgcagcccgc cagataaccc aggccaaaaa agagcgcacc cagaattaaa cctacagaaa
     4261 aattgctttg cagtaacggt tcataaacgt aaatcataaa cggtccggtc aagaccagaa
     4321 tgaaactcat acaccagatg agcggtttct tcagaccgag tttatcctga acgatgccgt
     4381 agaacatcat aaatagaatg ctggtaaact ggttgaccga ataaagtgta cctaattccg
     4441 tccctgttaa tcctagatgt cctttcagcc aaatagcgta taacgaccac cacagcgacc
     4501 aggaaataaa aaagagaaat gagtaactgg atgcaaaacg atagtacgca tttctgaatg
     4561 gaatattcag tgccataatt acctgcctgt cgttaaaaaa ttcatgtcct atttagagat
     4621 aagagcggcc tcgccgttta cttctcactt tccagttctt gtcgacatgg cagcgctgtc
     4681 attgcccctt tcgctgttac tgcaagcgct ccgcaacgtt gagcgagatc gataattcgt
     4741 cgcatttctc tctcatctgt agataacccc gtagaggaca gacctgtgag taacccggca
     4801 acgaacgcat ctcccgcccc agtgctatcg acacaattca cagacattcc agcaaaatgg
     4861 tgaacttgtc ctcgataaca gaccaccacc ccttctgcac ctttagtcac caacagcatg
     4921 gcgatctcat actcttttgc cagggcgcat atatcccgat cgttctgtgt ttttccactg
     4981 ataagtcgcc attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc
     5041 aaacacaagc ggagcaaatg ctcgtcttgc catagatctt cacgaatatt gggatcgaag
     5101 ctgacaaaac ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa
     5161 ggctcggcag acaacgcaat tgaacagaga tgtaaccatt cggcatgtcg ccagcagggc
     5221 aagtctgtcg tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt
     5281 tctccttgat cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc
     5341 agatacgtga tatcgacacc ctcagttagc agcgttcttt gcattaacgc accaaaagga
     5401 tcatcaccga cccgacctat aaacccactt gttccgccta atctggcgat tcccaccgca
     5461 acgttagctg gcgcgccgcc aggacaaggc agtagccgcc cgtctgattc tggcaagaga
     5521 tctacgaccg catcccctaa aacccatact ttggctgaca tttttttccc ttaaattcat
     5581 ctgacttacg catagtgata aacctctttt tcgcaaaatc gtcatggatt tactaaaaca
     5641 tgcatattcg atcacaaaac gtcatagtta acgttaacat ttgtgatatt catcgcattt
     5701 atgaaagtaa gggactttat ttttataaaa gttaacgtta acaattcacc aaatttgctt
     5761 aaccaggatg ataaaaatga cgcaatctcg attacatgcg gcgcaaaacg cactagcaaa
     5821 acttcacgag cgccgaggta acactttcta tccccatttt cacctcgcgc ctcctgccgg
     5881 gtggatgaac gatccaaacg gcctgatctg gtttaacgat cgttatcacg cgttttatca
     5941 acatcacccg atgagcgaac actgggggcc aatgcactgg ggacatgcca ccagcgacga
     6001 tatgatccac tggcagcatg agcctattgc gctagcgcca ggagacgaga atgacaaaga
     6061 cgggtgtttt tcaggtagtg ctgtcgatga caatggtgtc ctctcactta tctacaccgg
     6121 acacgtctgg ctcgatggtg caggtaatga cgatgcaatt cgcgaagtac aatgtctggc
     6181 taccagtcgg gatggtattc atttcgagaa acagggtgtg atcctcactc caccagaagg
     6241 catcatgcac ttccgcgatc ctaaagtgtg gcgtgaagcc gacacatggt ggatggttgt
     6301 cggggcgaaa gatccaggca acacggggca gatcctgctt tatcgcggca gttcattgcg
     6361 tgaatggacc ttcgatcgcg tactggccca cgctgatgcg ggtgaaagct atatgtggga
     6421 atgtccggac tttttcagcc ttggcgatca gcattatctg atgttttccc cgcagggaat
     6481 gaatgccgag ggatacagtt atcgaaatcg ctttcaaagt ggcgtaatac ccggaatgtg
     6541 gtcgccagga cgactttttg cacaatccgg gcattttact gaacttgata acgggcatga
     6601 cttttatgca ccacaaagct ttgtagcgaa ggatggtcgg cgtattgtta tcggctggat
     6661 ggatatgtgg gaatcgccaa tgccctcaaa acgtgaaggc tgggcaggct gcatgacgct
     6721 ggcgcgcgag ctatcagaga gcaatggcaa acttctacaa cgcccggtac acgaagctga
     6781 gtcgttacgc cagcagcatc aatctatctc tccccgcaca atcagcaata aatatgtttt
     6841 gcaggaaaac gcgcaagcag ttgagattca gttgcagtgg gcgctgaaga acagtgatgc
     6901 cgaacattac ggattacagc tcggcactgg aatgcggctg tatattgata accaatctga
     6961 gcgacttgtt ttgtggcggt attacccaca cgagaattta gatggctacc gtagtattcc
     7021 cctcccgcag ggtgacatgc tcgccctaag gatatttatc gatacatcat ccgtggaagt
     7081 atttattaac gacggggagg cggtgatgag tagccgaata tatccgcagc cagaagaacg
     7141 ggaactgtcg ctctatgcct cccacggagt ggctgtgctg caacatggag cactctggca
     7201 actgggttaa cataatatca ggtggaacaa cggatcaaca gcgggcaagg gatccgcgtc
     7261 actcttcccc cttcacgacc ttcaataata tgcaatgcag cttcccgccc gataatgtca
     7321 tgtggaagct gaattgtggt cagcggcggt aaaaacagat gcccgacgcc aaccagatta
     7381 tcaaagccca ttacggcgac atccttcggg atacgtaccc ccttcgccaa aagaacctga
     7441 taagccacaa aggctgcgcg atcgttacca catatcagaa catcaaaatc tggtttgccc
     7501 gatttgaagt ggtcattgag taaacttgcg agatcggtgt agtgatcatc acctgttgcc
     7561 atgtgaaatt gtttcacctc agccagatct cgtccagcat cacgccaggc ctgctcaaat
     7621 ccctgccgac gataccctgt tgccaacgca ctttccggta gccagaagca taacggttga
     7681 cgatagcccg ccgcgagcaa atgctgtgtt gattcatatt gtgcagtgta atcatcaggg
     7741 atataactgg gtaacgctgg gtcatccgcc acacagttcg ccaatacaat attttcacca
     7801 tacagagact caggcagcgt gatatgtcgc agccccattg tagtatagat aatgccatcc
     7861 ggacggtggg caagcagctg acgtgccgcg cgggcagcgt catcttcaga aaaaatattg
     7921 attaaaaaac tattccagcc gaactcgctg gcggtttgct caatggcaag cagaatatca
     7981 acagagaaag gagtggtagc agtgtcctgc gccagcacgg cgagagtcga cggcttacgt
     8041 ccttgagcgc gcatcttacg ggcggaaaga tcaggaacat aattcagggt ctggattgcc
     8101 tgcaatacgc gatcacgcgt tgcaggacgc acagattctg cattatgcat cacccgggag
     8161 actgtcatca tcgacactcc cgccaggcgt gcgacatcct ttaatgaagc catacccaag
     8221 ccgtttgccg taaaacgggc actgtagcag aaacagacgt cactggcgag atccaacgat
     8281 attctcgacg ccctatcacc cgacacggca atacaataaa aaataacaat aattcccgga
     8341 caattgtacc caattccgcc tctgttctcg ccaacgagta accatgtagg caagtaggta
     8401 aaacatgcca actggcatcg ccaatctgga gaagaaaata taaatatgga tcattgtgct
     8461 gccgctgtat cccgacatca gcccggaaat tattgcgatt gctatcggtt caggtgcgat
     8521 tggctgcacg atcgttacgg actcgctttt ctggctggcg aagcaatatt gcggcgctac
     8581 gctcaatgaa acatttaaat actatacgac agcgacattt atcgcttcag tcgtcgctct
     8641 ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa agagacgtac tatggaaaac
     8701 gctaaaatga attcgctcat cgcccagtat ccattggtaa aggatctggt tgctcttaaa
     8761 gaaaccacct ggtttaatcc tggcacgacc tcattggctg aaggtttacc ttatgttggc
     8821 ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc accctatctg
     8881 gcaaaagcat ttcctgaaac tgctgctact ggggggatta ttgaatcaga actggttgcc
     8941 attccggcta tgcaaaaacg gctggaaaag gaatatcaac aaccgatcag cgggcaactg
     9001 ctactgaaaa aagatagcca tttgcccatt tccggctcca taaaagcacg cggcgggatt
     9061 tatgaagtcc tggcacatgc agaaaaactg gctctggaag cggggttgct gacgcttgaa
     9121 gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca atacagcatt
     9181 gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc ccgcattggc
     9241 tttaaggtga cagtacatat gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc
     9301 agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgcagt cgaggaagga
     9361 cgtaacgcag cgcagtctga cccgaactgt ttctttattg atgacgagaa ttcccgcacg
     9421 ttgttccttg ggtattccgt cgcaggccag cgtcttaaag cgcaatttgc cgagcaaggt
     9481 cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt
     9541 cctggtggcg tcgcattcgg gcttaaactg gcatttggcg atcatgttca ctgctttttt
     9601 gccgaaccaa cgcactcccc ttgtatgctg ttaggcgtcc atacaggatt acacgatcag
     9661 atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct tgcagttggt
     9721 cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctac tggatggctt ctataccctt
     9781 agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg cattcgtctt
     9841 gaaccttcgg cactggcggg tatggccggg cctcagcgcg tgtgcgcatc agtaagttac
     9901 caacagatgc acggtttcag cgcagaacaa ctgcgtaatg ccactcatct ggtgtgggcg
     9961 acgggaggtg gaatggtgcc ggaagaagag atggagcaat atctggcaaa aggccgctaa
    10021 ttaattttca attcagcatc gccatccttt ccctgggtga gcgatgctgc cgatggcgca
    10081 gacttaaggt ccctggtctt acctgctata accccctcct tttatcatca cccaacgcct
    10141 ttcgctgtaa acggcggttt cgcaaaccaa accaaaactg taagtaagat aaaagcaata
    10201 gccgccatac ggaaaatctc atttgcagaa attgaaagtg actgctgagt tatttcatta
    10261 ttaatttcat taagaacccc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat
    10321 gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat
    10381 tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga
    10441 aaaaaattac tcatactcga ggcattggca aatttattat ctggcaaacc tgaaaacgaa
    10501 atcgttgtta agggtaaaaa gaaacaggcg acggcgaatc cctgaaaaaa ctgtggcaaa
    10561 atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc
    10621 gcatacatta aaaaactaaa tgtcaccaac aaccgcatat caattttgtt gccataacgt
    10681 cctatcaaag gtgatattaa tagcggcatg atgccgatgg gcgcataggc aagtccggcc
    10741 catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac tatcgcccca
    10801 gagtaaaata aatatgcgca tgtgatactc acaataccaa tggtgaaatt acgggactta
    10861 aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac taaagaaatc
    10921 agagaaataa ctgacattac tgttagtatt attattgtac tcgagttgaa ccaatccaga
    10981 tcgcgccctt tatcaagcat tatctgcaag ccaccaacac cgagcactaa cagggtcagt
    11041 cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag taaggttaag
    11101 cataatgtca gaacgataat ccccataggg acattgatta aaaatatcca accccagcta
    11161 aagttatcac aaatataacc gcccaatatc ggcccacata tcggcgcgat aatcacggtc
    11221 atagaccata atgccagagc aaatgttctt ttttctggtg gataattcct taataataaa
    11281 ctctgtgaca gtggaattaa cggccccgcc attaatccct gaacgactct aaaaaatatc
    11341 agcacatcaa gattggtcga taaactacac attaaggaag acagcgaaaa aaaagtgact
    11401 gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca gcctgccggt aacagggatc
    11461 gcaatggcat ttgctacgcc aaacgaagtg ataacccagg tgccttcgtc tgttgatgct
    11521 cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc
    11581 ataaacgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc ggttaatggt
    11641 gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa
    11701 tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa gcaggcatgg
    11761 acgtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc gtatcaatag
    11821 ttgctgtcat cgataaacca atacgtaagg gatgttccat gagttctttt ggatcaagag
    11881 aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag
    11941 gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca
    12001 caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc cgtacatccg
    12061 tgagttgtgt ttctttaaag ttagcattaa cccacatttg acgtgccggt acgacagcca
    12121 ttaacgattg tccgggactc actgtttcgc cgacctgaac acttcgctgg gcaatgtagc
    12181 cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct ttggttgcat
    12241 ccgcagcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact aaagctttat
    12301 tcgctttata agcctgaata gcagcattca atgccgcttt gctacttatt aacgtatctt
    12361 tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaaaggt acacgacggt
    12421 tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt
    12481 gtttatcctg taagtacagt ttattcgttt gccgaacaat atttgccaga ttatttttag
    12541 ctttattgag tgcgatagtg gcatcagtct tatccagtga gactaaaatg tcaccctgtc
    12601 gaacgtagtt cgtatctt
//
LOCUS       DARNVM010000145        12281 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.145, whole genome shotgun sequence.
ACCESSION   DARNVM010000145 DARNVM010000000
VERSION     DARNVM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.145"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(210..1493)
                     /gene="gltA"
                     /locus_tag="SI621_003679"
     CDS             complement(210..1493)
                     /gene="gltA"
                     /locus_tag="SI621_003679"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEI4066341.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1699..1806)
                     /locus_tag="SI621_003680"
     CDS             complement(1699..1806)
                     /locus_tag="SI621_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066342.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGKQV"
     gene            2187..2591
                     /gene="sdhC"
                     /locus_tag="SI621_003681"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2187..2591
                     /gene="sdhC"
                     /locus_tag="SI621_003681"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HEI4066343.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2585..2932
                     /gene="sdhD"
                     /locus_tag="SI621_003682"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2585..2932
                     /gene="sdhD"
                     /locus_tag="SI621_003682"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HEI4066344.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2932..4698
                     /gene="sdhA"
                     /locus_tag="SI621_003683"
     CDS             2932..4698
                     /gene="sdhA"
                     /locus_tag="SI621_003683"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEI4066345.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNADGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4714..5430
                     /gene="sdhB"
                     /locus_tag="SI621_003684"
     CDS             4714..5430
                     /gene="sdhB"
                     /locus_tag="SI621_003684"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HEI4066346.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5780..8581
                     /gene="sucA"
                     /locus_tag="SI621_003685"
     CDS             5780..8581
                     /gene="sucA"
                     /locus_tag="SI621_003685"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEI4066347.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8596..9813
                     /gene="odhB"
                     /locus_tag="SI621_003686"
     CDS             8596..9813
                     /gene="odhB"
                     /locus_tag="SI621_003686"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEI4066348.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSTGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9907..11073
                     /gene="sucC"
                     /locus_tag="SI621_003687"
     CDS             9907..11073
                     /gene="sucC"
                     /locus_tag="SI621_003687"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEI4066349.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11073..11942
                     /gene="sucD"
                     /locus_tag="SI621_003688"
     CDS             11073..11942
                     /gene="sucD"
                     /locus_tag="SI621_003688"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEI4066350.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIIIITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEVLKTVLK"
BASE COUNT         2963 a         3132 c         3346 g         2840 t
ORIGIN      
        1 attttcattg ggttaacaat gctaccggca ggaaagtata aaaataaact tatcattctg
       61 ctcagtatgt gacttgtcac attatttcac cttattaatg ggtatttgca tctggtttaa
      121 tttttacaat ttattatgtt cagccatata aaaaagaacc cgctctatta aaggcgggtc
      181 cggaaagtaa acggcttagc aatcaaccat taacgcttaa tgtcgctttt aaagtcgcgt
      241 ttttcatatc ctgtatacag ttgacgcgga cgggcaatct tcataccgtc actgtgcatt
      301 tcgctccagt gggcgatcca gccaacggta cgtgccatag cgaagatcac ggtgaacata
      361 gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac gttcgggtag
      421 agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc catagccact
      481 tccagcaggt catctttggt acccagctct ttcagcactt catggcaggt ttcacgcatt
      541 acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat caggcggaaa
      601 gagtcatttt tgtctttcgc acgacgaaca aattccggaa tgtgtttaac ggagctgatt
      661 tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gcgcaggtcc ccacagtgaa
      721 gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc ggtacgcacg
      781 gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg gtccatagca
      841 cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat catattcagg
      901 aagttaccgg cataggagag atcgttgcgc gggtaaacaa atggctgacc aatggaatac
      961 ttgtaacaca tcgcggccat ggtcggcatt ttcgacagca ggcggaacgc ggcaatttca
     1021 cgatgacgag gattgttaac atccagcgag tcgtgataga acgccgccag cgcgccggta
     1081 ataccacaca tgactgccat tggatgcgag tcgcgacgga aagcatggaa cagacgggta
     1141 atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc atactgttcc
     1201 tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt agaatcggtc
     1261 gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc atcaataaaa
     1321 gtaattttag attcgcagga tgcggttgaa gtgaagcctg ggtcaaaggt gaacacacct
     1381 tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt cagcacatcc
     1441 agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc catttaaggt
     1501 ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg cacatcaacc
     1561 tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa acctgggtac
     1621 agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg cgaatcaagt
     1681 acttagcaat ccgaattatt aaacttgttt accactaata actgtcccga atgaattggt
     1741 caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt cataactttt
     1801 acgcattata tgcttttcct ggtaatgttt gtaacaactt tgttgaatga ttgtcaaatt
     1861 agatgattaa aaattaaata aatgttgtta tcgtgacctg gatcactgtt caggataaaa
     1921 cccgacaaac tatatgtagg ttaattgtaa tgattttgtg aacagcctat actgccgcca
     1981 ggtctccgga acaccctgca atcccgagcc acccagcgtt gtaacgtgtc gttttcgcat
     2041 ctggaagcag tgttttgcat gacgcgcagt tatagaaagg acgctgtctg acccgcaagc
     2101 agaccggagg aaggaaatcc cgacgtctcc aggtaacaga aagttaacct ctgtgcccgt
     2161 agtccccagg gaataataag aacagtatgt gggcgttatt catgataaga aatgtgaaaa
     2221 aacaaagacc tgttaatctg gacctacaga ccatccggtt ccccatcacg gcgatagcgt
     2281 ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc agtgggcatc ctgctgtggc
     2341 ttctgggtac cagcctctct tcccctgaag gtttcgagca agcttccgcg attatgggca
     2401 gcttcttcgt caaatttatc atgtggggca tccttaccgc tctggcgtat cacgtcgtcg
     2461 taggtattcg ccacatgatg atggattttg gctatctgga agaaacattc gaagcgggta
     2521 aacgctccgc caaaatctcc tttgttatta ctgtcgtgct ttcacttctc gcaggagtcc
     2581 tcgtatggta agcaacgcct ccgcattagg acgcaatggc gtacatgatt ttatcctcgt
     2641 tcgcgctacc gctatcgtcc tgacgctcta catcatttat atggtcggtt ttttcgctac
     2701 cagtggcgag ctgacatatg aagtctggat cggtttcttc gcctctgcgt tcaccaaagt
     2761 gttcaccctg ctggcgctgt tttctatctt gatccatgcc tggatcggca tgtggcaggt
     2821 gttgaccgac tacgttaaac cgctggcttt gcgcctgatg ctgcaactgg tgattgtcgt
     2881 tgcactggtg gtttacgtga tttatggatt cgttgtggtg tggggtgtgt gatgaaattg
     2941 ccagtcagag aatttgatgc agttgtgatt ggtgccggtg gcgcaggtat gcgcgcggcg
     3001 ctgcaaattt cccagagcgg tcagacctgt gcgctgctct ctaaagtctt cccgacccgt
     3061 tcccataccg tttctgcgca aggcggcatt accgttgcgc tgggtaatac ccatgaagat
     3121 aactgggaat ggcatatgta cgacaccgta aaaggttcgg actatatcgg tgaccaggac
     3181 gcgattgaat atatgtgtaa aaccgggccg gaagcgattc tggagctcga acacatgggc
     3241 ctgccgttct cgcgccttga tgatggtcgt atctatcaac gtccgtttgg cggccagtcg
     3301 aaaaacttcg gcggcgagca ggcggcacgc actgcggcgg cagctgaccg taccggtcac
     3361 gcactgttgc acacccttta tcagcagaac ctgaaaaacc acaccactat tttctccgaa
     3421 tggtatgcgc tggatctggt gaaaaacgcc gatggcgcgg tggtcggttg taccgcactg
     3481 tgcatcgaaa ctggtgaagt ggtttatttc aaagcccgcg ctaccgtgct ggcgactggc
     3541 ggggcagggc gtatttatca gtccaccacc aacgcccaca ttaacactgg cgacggcgtc
     3601 ggcatggcta tccgtgcagg cgtaccagta caggatatgg aaatgtggca gttccacccg
     3661 accggcattg caggagcggg cgtactggtc accgaaggtt gccgtggtga aggcggttat
     3721 ctgctgaaca aacatggcga acgctttatg gagcgttatg cgccgaacgc caaagacctg
     3781 gcgggccgtg acgtggtggc gcgttccatc atgatcgaaa tccgtgaagg tcgcggctgt
     3841 gatggtccgt gggggccaca cgcaaaactg aaacttgacc acctgggtaa agaagttctc
     3901 gaatcccgtc tgccgggtat cctggaactc tcccgtacct tcgctcacgt tgatccggtg
     3961 aaagagccga ttccggttat cccaacctgt cactacatga tgggcggtat tccgaccaaa
     4021 gtgaccggtc aggcgctgac tgtgaacgag aaaggcgaag atgtggttgt tccggggctg
     4081 tttgccgttg gtgaaatcgc ttgtgtatcg gtacacggcg ctaaccgtct gggcggcaac
     4141 tcgctgctgg acctggtggt ctttggtcgt gcggcaggtc tgcatctgca agagtctatc
     4201 gccgagcagg gcgcactgcg cgatgccagc gagtctgatg ttgaagcgtc tctggatcgt
     4261 ctgaaccgct ggaacaataa tcgtaacggt gaagatccgg tggcgattcg taaagcgctg
     4321 caagaatgta tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat ggcgaaaggg
     4381 cttgagcagt tgaaagtgat ccgcgagcgt ctgaaaaatg cccgtctgga tgacacttcc
     4441 agcgagttca acacccagcg cgttgagtgc ctggaactgg ataacctgat ggaaacggcg
     4501 tatgcaacgg ctgtttctgc caacttccgt accgaaagcc gtggcgcgca tagccgcttc
     4561 gacttcccgg atcgtgatga tgaaaactgg ctgtgccact ccctgtatct gccagagtcg
     4621 gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc attcccgccg
     4681 aagattcgta cttactaatg cggagacagg aaaatgagac tcgagttttc aatttatcgc
     4741 tataacccgg atgttgatga tgctccgcgt atgcaggatt acaccctgga agcggaagaa
     4801 ggtcgtgaca tgatgctgct ggatgcgctt atccagctga aagagaaaga tcccagcctg
     4861 tcgttccgtc gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa catgaacggc
     4921 aagaatggtc tggcctgtat taccccgatt tcggcactca accagccggg caagaagatt
     4981 gtgattcgcc cgctgccagg tttaccggtg atccgcgatt tggtggtaga catgggacaa
     5041 ttctatgcgc aatatgagaa aattaagcct tacctgttga ataatggaca aaatccgcca
     5101 gctcgcgagc atttacagat gccagagcag cgcgaaaaac tcgacgggct gtatgaatgt
     5161 attctctgcg catgttgttc aacctcttgt ccgtctttct ggtggaatcc cgataagttt
     5221 atcggcccgg caggcttgtt agcggcatat cgtttcctga tcgatagccg tgataccgag
     5281 actgacagcc gcctcgacgg tttgagcgat gcattcagtg tattccgctg tcacagcatc
     5341 atgaactgcg tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat cggccatatc
     5401 aagtcgatgt tgttgcagcg taatgcgtaa acccaggcct gataagacgc acaagcgtcg
     5461 catcaggcaa ccagtgccgg atgcggcgtg aacgccttat ccggcctaca aaagaaatgc
     5521 agaaatcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaacagc gccggatgcg
     5581 gcgtgaatgc cttatccggc ctacaaaaga aatgcaggaa atctttaaaa actgcccctg
     5641 acactaagac agtttttaaa ggttccttcg cgagccacta cgtagacaag agctcgcaag
     5701 tgaaccccgg cacgcacatc actgtgcgtg gtagtatcca cggcgaagta agcataaaaa
     5761 agatgcttaa gggatcacga tgcagaacag cgctttgaaa gcctggttgg actcttctta
     5821 cctctctggc gcaaaccaga gctggataga acagctctat gaagacttct taaccgatcc
     5881 tgactcggtt gacgctaact ggcgttcgac gttccagcag ttacctggta cgggagtcaa
     5941 accggatcaa ttccactctc aaacgcgtga atatttccgc cgcctggcga aagacgcttc
     6001 acgttactct tcaacgatct ccgaccctga caccaatgtg aagcaggtta aagtcctgca
     6061 gctcattaac gcataccgct tccgtggtca ccagcatgcg aatctcgatc cgctgggact
     6121 gtggcagcaa gataaagtgg ccgatctgga tccgtctttc cacgatctga ccgaagcaga
     6181 cttccaggag accttcaacg tcggttcatt tgccagcggc aaagaaacca tgaaactcgg
     6241 cgagctgctg gaagccctca agcaaaccta ctgcggcccg attggtgccg agtatatgca
     6301 tattaccagc accgaagaaa aacgctggat ccaacagcgt atcgagtctg gtcgcgcgac
     6361 tttcaatagc gaagagaaaa aacgcttctt aagcgaactg accgccgctg aaggccttga
     6421 acgttacctc ggtgcaaaat ttcctggtgc aaaacgcttc tcgctggaag gcggtgacgc
     6481 gttaatcccg atgcttaaag agatgatccg ccacgctggc aacagcggca cccgcgaagt
     6541 ggttctcggg atggcgcacc gtggtcgtct gaacgtgctg gtgaacgtgc tgggtaaaaa
     6601 accgcaagac ttgttcgacg agtttgccgg taaacataaa gaacacctcg gcacgggcga
     6661 cgtgaaatac cacatgggct tctcgtctga cttccagacc gatggcggcc tggtgcacct
     6721 ggcgctggcg tttaacccgt ctcaccttga gattgtcagc ccggtagtta tcggttctgt
     6781 tcgtgcccgt ctggacagac ttgatgagcc gagcagcaac aaagtgctgc caattaccat
     6841 ccacggtgac gccgcagtga ccgggcaggg cgtggttcag gaaaccctga acatgtcgaa
     6901 agcgcgtggt tatgaagttg gcggtacggt acgtatcgtt atcaacaacc aggttggctt
     6961 caccacctct aatccgctgg atgctcgttc tacaccgtac tgtactgata tcggtaagat
     7021 ggttcaggcc ccgattttcc acgttaacgc ggacgatccg gaagccgttg cctttgtgac
     7081 ccgtctggcg ctcgatttcc gtaacacctt taaacgtgat gtcttcatcg acctggtgtg
     7141 ctaccgccgt catggccata acgaagccga cgagccgagc gcaacccagc cgctgatgta
     7201 tcagaaaatc aaaaaacatc cgacgccgcg caaaatctac gctgacaagc tggagcagga
     7261 aaaagtcgcg acgctggaag atgccaccga gatggttaac ctgtaccgcg atgcgctgga
     7321 tgctggcgat tgcgtggtag cagagtggcg tccgatgaac atgcactctt tcacctggtc
     7381 accgtacctc aaccacgaat gggacgaaga gtacccgaac aaagttgaga tgaagcgcct
     7441 gcaggaactg gctaaacgca tcagcacggt gccggaagca gttgaaatgc agtctcgcgt
     7501 tgccaagatt tatggcgatc gccaggcaat ggcagccggt gagaaactgt tcgactgggg
     7561 cggcgcggaa aacctcgctt acgccacgct ggttgatgaa ggcattccgg ttcgcctgtc
     7621 gggtgaagac tccggtcgcg gtaccttctt ccaccgccac gcggtgatcc acaaccagtc
     7681 taacggttcc acttacacgc cgctgcaaca catccataac ggccagggcg cgttccgtgt
     7741 ctgggactcc gtactgtcag aagaagccgt gctggcgttt gaatacggtt atgccaccgc
     7801 agaaccacgc actctgacta tctgggaagc acagttcggt gacttcgcta acggtgctca
     7861 ggtggttatc gaccagttca tctcctctgg cgaacagaaa tggggccgga tgtgtggtct
     7921 ggtgatgttg ctgccgcacg gttacgaagg gcaggggccg gagcactcct ccgcgcgtct
     7981 ggaacgttat ctgcaacttt gcgctgagca aaacatgcag gtgtgcgtac cgtctacccc
     8041 ggcacaggtt taccacatgc tgcgtcgtca ggcgctgcgc gggatgcgtc gtccgctggt
     8101 cgtgatgtcg ccgaaatccc tgctgcgtca tccgctggcg gtttccagcc tcgaagaact
     8161 ggcgaacggc accttcctgc cagccatcgg tgaaatcgac gagcttgatc cgaagggcgt
     8221 gaagcgcgta gtgatgtgtt ctggtaaggt ttattacgac ctgctggaac agcgtcgtaa
     8281 gaacaatcaa cacgatgtcg ccattgtgcg tatcgagcaa ctctacccgt tcccgcataa
     8341 agcgatgcag gaagtgttgc agcagtttgc tcacgtcaag gattttgtct ggtgccagga
     8401 agagccgctc aaccagggcg catggtactg cagccagcat catttccgtg aagtgattcc
     8461 gtttggggct tctctgcgtt atgcaggccg cccggcctcc gcctctccgg cggtagggta
     8521 tatgtccgtt caccagaaac agcaacaaga tctggttaat gacgcgctga acgtcgaata
     8581 aataaaggat acacaatgag tagcgtagat attctggtcc ctgacctgcc tgaatccgta
     8641 gccgatgcca ccgtcgcaac ctggcataaa aaacccggcg acgcagtcgt acgtgatgaa
     8701 gtgctggtag aaatcgaaac tgacaaagtg gtactggaag taccggcatc agcagacggc
     8761 attctggatg cggttctgga agatgaaggt acaacggtaa cgtctcgtca gatccttggt
     8821 cgcctgcgtg aaggcaacag caccggtaaa gaaaccagcg ccaaatctga agagaaagcg
     8881 tccactccgg cgcaacgcca gcaggcgtct ctggaagagc aaaacaacga tgcgttaagc
     8941 ccggcgatcc gtcgcctgct ggctgaacac aacctcgacg ccagcgccat taaaggcacc
     9001 ggtgtgggtg gtcgtctgac tcgtgaagat gtggaaaaac atctggcgaa agccccggcg
     9061 aaagagtctg ctccggcagc ggctgctccg gcggcgcaac cggctctggc tgcacgtagc
     9121 gaaaaacgtg tgccgatgac tcgcctgcgt aagcgtgtgg cagagcgtct gcttgaagcg
     9181 aaaaactcca ccgctatgct gaccacgttc aacgaagtca acatgaagcc gattatggat
     9241 ctgcgtaagc agtacggtga agcgtttgaa aaacgtcacg gtattcgtct gggctttatg
     9301 tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt acccggaagt gaatgcttct
     9361 atcgacggcg atgatgtggt ttaccacaac tatttcgacg tcagcatggc ggtttctacg
     9421 ccgcgcggcc tggtgacgcc ggtactgcgt gatgtcgata ccctaggcat ggcagacatc
     9481 gagaagaaaa tcaaagagct ggcagttaaa ggccgtgacg gcaagctgac ggttgaagat
     9541 ctgaccggtg gtaacttcac catcaccaat ggtggtgtgt tcggttccct gatgtctacg
     9601 ccgatcatca acccgccgca gagcgcaatt ctgggtatgc acgctatcaa agatcgtccg
     9661 atggcggtga atggtcaggt tgagatcctg ccgatgatgt acctggcgct gtcctacgat
     9721 caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg taacgatcaa agagttgctg
     9781 gaagatccga ctcgtctgct gctggacgtg tagtagttta agcttcacct gcactgtaga
     9841 ccgacgtgga tagggtgtcg gtctacggtt taaaagataa cgattactga aggatggaca
     9901 gaacacatga acttacatga atatcaggca aaacaacttt ttgcccgcta tggcttacca
     9961 gcaccggtgg gttatgcctg tactactccg cgcgaagcag aagaagccgc ttcaaaaatc
    10021 ggtgccggtc cgtgggtagt gaaatgtcag gttcacgctg gtggccgcgg taaagcgggc
    10081 ggtgtgaaag tggtaaacag caaagaagac atccgtgctt ttgcagaaaa ctggctgggc
    10141 aagcgtctgg taacgtatca aacagatgcc aatggccaac cggttaacca gattctggtt
    10201 gaagcagcga ccgatatcgc taaagagctg tatctcggtg ccgttgttga ccgtagttcc
    10261 cgtcgtgtgg tctttatggc ctccaccgaa ggcggcgtgg aaatcgaaaa agtggcggaa
    10321 gaaactccgc acctgatcca taaagttgcg cttgatccgc tgactggccc gatgccgtat
    10381 cagggacgcg agctggcgtt caaactgggt ctggaaggta aactggttca gcagttcacc
    10441 aaaatcttca tgggcctggc gaccattttc ctggagcgcg acctggcgct gatcgaaatc
    10501 aacccgctgg tcatcactaa acagggcgat ctgatttgcc tcgacggcaa actgggtgct
    10561 gacggcaacg cactgttccg ccagcctgat ctgcgcgaaa tgcgtgacca gtcacaggaa
    10621 gatccgcgtg aagcacaggc tgcacagtgg gaactgaact acgttgcgct ggacggtaac
    10681 atcggttgta tggttaacgg cgcaggtctg gcgatgggta cgatggacat cgttaaactg
    10741 cacggcggcg aaccggctaa cttcctggac gttggcggcg gcgcaaccaa agaacgtgta
    10801 accgaagcat tcaaaatcat cctctctgac gacaaagtga aagccgttct ggttaacatc
    10861 ttcggcggta tcgttcgttg cgacctgatc gctgacggta tcatcggcgc ggtagcggaa
    10921 gtgggtgtta acgtaccggt cgtggtacgt ctggaaggta acaacgccga actcggcgcg
    10981 aagaaactgg ctgacagcgg cctgaatatt attgcagcaa aaggtctgac ggatgcagct
    11041 cagcaggttg ttgccgcagt ggaggggaaa taatgtccat tttaattgat aaaaacacca
    11101 aggttatctg ccagggcttt accggtagcc aggggacttt ccactctgaa caagccattg
    11161 catacggcac taaaatggtt ggcggtgtaa ccccaggtaa aggcggcacc acccacctcg
    11221 gcctgccggt gttcaacacc gtgcgtgaag ctgttgctgc tactggcgct accgcttctg
    11281 ttatctacgt accagcaccg ttctgcaaag actccattct ggaagccatc gatgcaggca
    11341 tcaaactgat tatcatcatc actgaaggca tcccgacgct ggatatgctg actgtgaaag
    11401 tgaaactgga cgaagctggc gttcgtatga tcggcccgaa ctgcccaggc gttatcactc
    11461 cgggtgaatg caaaatcggt atccagccgg gccacattca caaaccgggt aaagtgggta
    11521 tcgtttcccg ttccggtaca ctgacctatg aagcggttaa acagaccacg gattacggtt
    11581 tcggtcagtc gacctgtgtc ggtatcggcg gtgacccgat ccctggctct aactttatcg
    11641 acatcctcga aatgttcgaa aaagatccgc agaccgaagc gatcgtgatg atcggtgaga
    11701 tcggcggtag cgctgaagaa gaagcggctg cgtacatcaa agagcacgtt accaagccag
    11761 ttgtaggtta catcgctggt gtgactgcgc cgaaaggcaa acgtatgggc cacgcgggtg
    11821 ccatcattgc cggtgggaaa gggactgcgg atgagaaatt cgctgctctg gaagccgcag
    11881 gcgtgaaaac cgttcgcagc ctggcggata tcggtgaagt actgaaaact gttctgaaat
    11941 aaatatctgt aataagaaat agccctcgcc gcttccctct acaggaatgg cgaagggctg
    12001 tcggtttcga catggttggc catcgtatga tggccttttt tatgccttaa atgttaacca
    12061 cggtcgaaat ctgaccgctg gcttgactgc atgaccacag ccactgtgga ttgccgcaac
    12121 cgaccgcaag ccacgaataa gaacgacggc gaatttcgaa tcgttacctg atgcactggc
    12181 ttcccagcgt ttgaccgtag cgttaagtaa atcaacaaaa gcgatgttca ccgcttatcg
    12241 tcaagtcgtg ccatttgtca gccaggatcg ggtctttacc c
//
LOCUS       DARNVM010000146        12048 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.146, whole genome shotgun sequence.
ACCESSION   DARNVM010000146 DARNVM010000000
VERSION     DARNVM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.146"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            69..635
                     /gene="slp"
                     /locus_tag="SI621_003689"
     CDS             69..635
                     /gene="slp"
                     /locus_tag="SI621_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HEI4066351.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            788..1318
                     /locus_tag="SI621_003690"
     CDS             788..1318
                     /locus_tag="SI621_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4066352.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1379..2407)
                     /gene="chuS"
                     /locus_tag="SI621_003691"
     CDS             complement(1379..2407)
                     /gene="chuS"
                     /locus_tag="SI621_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HEI4066353.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRSERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     TPLELKAGDAPVVQMRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2456..4438)
                     /gene="chuA"
                     /locus_tag="SI621_003692"
                     /gene_synonym="shuA"
     CDS             complement(2456..4438)
                     /gene="chuA"
                     /locus_tag="SI621_003692"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HEI4066354.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSASDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            5121..6035
                     /gene="shuT"
                     /locus_tag="SI621_003693"
     CDS             5121..6035
                     /gene="shuT"
                     /locus_tag="SI621_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HEI4066355.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATYQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            6055..7392
                     /locus_tag="SI621_003694"
     CDS             6055..7392
                     /locus_tag="SI621_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HEI4066356.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIINILREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            7405..7899
                     /gene="hutX"
                     /locus_tag="SI621_003695"
     CDS             7405..7899
                     /gene="hutX"
                     /locus_tag="SI621_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HEI4066357.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            7899..8522
                     /gene="chuY"
                     /locus_tag="SI621_003696"
     CDS             7899..8522
                     /gene="chuY"
                     /locus_tag="SI621_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HEI4066358.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVVRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGQDALIISTMGGTQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            8607..9563
                     /gene="chuU"
                     /locus_tag="SI621_003697"
     CDS             8607..9563
                     /gene="chuU"
                     /locus_tag="SI621_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HEI4066359.1"
                     /translation="MGLSLLLLALVLFGASQGALRISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIMATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLISSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            9560..10330
                     /locus_tag="SI621_003698"
     CDS             9560..10330
                     /locus_tag="SI621_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEI4066360.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPASHSPLIVLDH"
     gene            complement(10382..11029)
                     /locus_tag="SI621_003699"
     CDS             complement(10382..11029)
                     /locus_tag="SI621_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEI4066361.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLNHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11093..11419)
                     /gene="hdeB"
                     /locus_tag="SI621_003700"
     CDS             complement(11093..11419)
                     /gene="hdeB"
                     /locus_tag="SI621_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HEI4066362.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(11535..11867)
                     /gene="hdeA"
                     /locus_tag="SI621_003701"
     CDS             complement(11535..11867)
                     /gene="hdeA"
                     /locus_tag="SI621_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HEI4066363.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         3100 a         2979 c         2832 g         3137 t
ORIGIN      
        1 cagaatcaga tgaaaactat aaagaaatat ctattatggt tttaatattt gttgataagg
       61 atagtaacat gaacatgaca aaaggtgcac tcatcctcag cctttcattt ttgcttgccg
      121 catgtagttc aattccgcaa aatatcaaag gcaataacca acctgatatt caaaaaagtt
      181 ttgttgctgt tcataaccag ccggggttat atgttggtca acaagcgcgc tttggtggga
      241 aggttatcaa cgttatcaat ggcaaaacgg atacgttgtt agaaatcgct gtattaccgt
      301 tggatagcta tgcgaagcct gatattgaag ccaactatca gggccgactg ctcgccagac
      361 aaagcggctt ccttgatcca gtgaactatc gtaatcactt tgttaccatc ctcggtacca
      421 ttcagggtga acaacctggc tttatcaata aagtcccgta taacttcctc gaagtgaata
      481 tgcagggcat ccaggtgtgg catttgagag aagtggttaa taccacctat aacctgtggg
      541 attacggcta tggtgcattc tggccggaac cgggctgggg cgcgccttac tacaccaatg
      601 cggtgagtca ggtaacacct gagctggtca aataacacca ccgagagatg cagatgcttt
      661 ctcagcatct gcatcatgca ttacatcaaa ttaatacaca gccagctaac tattattata
      721 agccctgtcc tgttaattac ctttggcaaa ctgattataa agttaatgtc cgcaccagga
      781 gtcggttatg tttcttataa ttaccaggga tacgatgttc ttcactgcga tgaaaaacat
      841 tctgagtaaa ggtaatgtcg ttcatataca gaacgaagaa gagatcgacg taatgttgca
      901 tcagcatgcc ttcgtcatta ttgatacatt aatgaataat gtatttcatt ctaatttgct
      961 cactcaaatt gaacgattaa aacctgtcca tgtgattatt ttctccccct ttaatattaa
     1021 acgctgcctg gggaaagtac cggtgacctt tgttccgcga actatcacca tcatcgactt
     1081 tgtcgcactc atcaatggca gttactgctc tgtgcctgaa gcggatgtgt ccctttcgcg
     1141 caagcagcat caggttctga gctgcattgc gaatcaaatg acaacggaag atattctgga
     1201 gaaactgaaa atatcgctaa aaacgttcta ctgccataaa cacaatatca tgatgatcct
     1261 caatcttaag cggatcaatg agttggtacg ccatcagcat attgattatc tggtgtgatc
     1321 gattgattaa tataaagagg cccagcaata aatagccaga cctcccgtta attatacgtt
     1381 atgcagtaac gccttccggt atcagcgaag caatttgctt acgccattgc gcttgctcct
     1441 gttcgccttc tgtacgttga ccataaagtt gcgctatctg cgtaccatca tgggcaaaca
     1501 attccagact ggtcacataa ccgtcactag caggtttacg ggttacccag gtttcagcaa
     1561 tgctctcttc taatagatga agagtaaacg tcgggttaaa aatattcagc caacctttca
     1621 ttggcaccac tttttctacc acgccagtga aaatctgtac gcagccacgg ttgccaacaa
     1681 acaccatgat ttcattgcca tcctgctgtg cagattcaag aatttgcgcc aacgcactgt
     1741 tggatacttt gcaggccaaa tcgtctgcca ccagattgaa cgcctgttgg cgcgtcaggt
     1801 tgtggcgctt gagcaacgta aaaaactgat gaacgtcggt catcgcccgc cactcttgct
     1861 caaccacact ggcatcggct cgcatttgaa cgactggcgc atcaccggct tttaactcaa
     1921 gcggcgtatt ctcatcggcg ataaaccgtg ccagaagctc actccatgcc gccatatcgg
     1981 tattgtcggt ggcataaact tttagtaatg catcgccctg atgatcaaag aactgaatac
     2041 tctggcgttc gctacgagtc gtattttctt taatgtgaaa aacactggcc cattgattga
     2101 gaaacagacg caaatccagc gcgcgtggat tgaggatcaa tccggcatgt ccgttcaaat
     2161 gctggtttgt gaacgcacca acttgctcat ggactgcata ttcattacga caaatacatt
     2221 tggtctcgcc aacactttcg agcgccgcca gaatgtcacg gatatcgccg cgcatccgcc
     2281 acgcatcgtg cgtgacgcgt gcaaatgcca gttctgcttc gctaatattc attaaacctg
     2341 cgatgtcacg cgcgtacttt ccgggatttt gttcttttaa ctcaagccag cgtgtgtagt
     2401 ggttcattgt ctctcttcct tccagataaa tgccccgaaa tatcggggca gatgattacc
     2461 attgataact cacgaaaatt tttccattac gaccatcctg tgggatgcct tgtggcgacc
     2521 agtactcttt gtcgaaagcg ttacccaaca ccaaagtagt ggtcatacct ttgagcgcct
     2581 gttgtccttg ataactgacg tagaaatcat tcacgccata gcctggttgt ttgctgtaac
     2641 tgctgctgat atgtgttgag cgatcggcaa acgtaccaac ccacccaaca gagaagccac
     2701 tgtgagcgat cggaatattc agggtgctgg taacggtatc cgggttaata ctggagatat
     2761 attcgccggt atcggtgtct ttgccgcggg tacggttata ggccacatca aggctaaaca
     2821 gatcagtggt atatttcgtc atcacatccc agccccagat tttggcgttc gggacgttat
     2881 acgacatagt tgtcgccgcc gcgaaatcga cggtcgtgga gatgtaatcc ttcgctttgg
     2941 tatcaaagta gctggcttta aattccaggg catcattgga caacatcagg tcatcaaaac
     3001 gcagcccaaa accgtactcc tgagtttcgt tagtttccgg acgtaagttc gggtttggca
     3061 cccaatagtt ggtatagaag cgaccaatcg agaagtgctt agaatcgtta tacatttcgc
     3121 ccatcgtcgg ggcgcggaat gcctgggcat atgagccaaa taacatcagc cagttagtcg
     3181 ggttgatagt catccccgcg cgagatgacc atttgtcggc atcaacatct ttgtagccgt
     3241 cgctgctacc gcgataactg tcatagcggg ttccgccaag cagagtaatc ggcagatctc
     3301 gtaaggtgat ctcatcctgt agccagccgg agctaaaatc gatttttgct tgcgggaagc
     3361 ccgtcgtcgc gccgcccgga tgttgttcct gacgataata ctcaccgcca tatgtcagta
     3421 agtgagaagc gaaactgtcg gcaaatagag tggaacggtt ctccagcctg gctccttttg
     3481 ttatctgttc acgatactcg ccggaactcc ccgtgttttg cgcattaata cggacttccg
     3541 accagtaaat ttttgcatct gcatttaacc agtcgttgcc ctgcggggcg agtttataag
     3601 aaagctgcgc atcgcgttga attgttgaac gatcaaccat cgggttgctg ctatcagaag
     3661 cttcaacggt ctgcggattt tttggttcac gcgcgtcgtt gttgtagtaa cgcactaaac
     3721 cgctcagaga ctgggctgaa tcaatttgcc aggtcccttt cgccagcatg ttattaatgg
     3781 actcgtcatt cggcgcggtt tcaccattgc tctggcgtaa atcaccccga tcgcgactgg
     3841 accaggccac aataccatcc agattttcag ttcgcccaaa cgcgctcgcg cctaatccca
     3901 ggctatggtc ccccgtgccg ccagtaccaa agacacgaaa accactgctt tgtccttcct
     3961 gcaataaatc ttttgcatcg accgtatcgt aggagatcac tccacccagc gcaccactgc
     4021 catacagtaa tgctgaaggt ccgcgaacaa tctcaacacg cttgatcagc gctggatcga
     4081 gaaaagtgcc attcaggtgt ccggtatccg ttccctggcg aacaccatcg acaagaacca
     4141 gcacgccgcg atgatcatag ccacgcatat ttacatcctg gccgttggtt cgtccggtac
     4201 catcaagagt aattccagga acatgacgca gcagatcgga ggctgaagta gccgtttgat
     4261 tttcaggagc ggaagtgtcg atgacgctga ccatcatagg cgcttcgaag gaactacggg
     4321 cattccccgt tgccgtaacg gtcatggttt cagtagcaaa agcaaacgtt ggcaaggtgg
     4381 cagaaacagc caaagccaac aaactcaaac gcaacgaggt aaattgcgga cgtgacatag
     4441 cgattctcca tgaggaaaat aaaaaatgct ggctgcctgg gttaataccg gggtggctgg
     4501 cgaaaaataa atttatttaa aattcagagc gataatattc gccgggtgag tttacaggcg
     4561 tttatatccc atgaaattgc ctggtatctc ggcaaatacg caaaagcacg ccctctttgt
     4621 caggcaaaac gatatgcccc atcaggagtg aagagcgctg tgcgttatcc cttgcaaatg
     4681 ctatggtttt gttatccata tggtcgtgtt ttgttaagcg caaacgtgat aatgagaatt
     4741 attatcgatc aattttttat gatcaagctt tttttaaccg ccattaacac aataaacagg
     4801 gggttatgaa acctatcccg cgccctgaaa accactttat ttttatggtt attcccccat
     4861 gacctcatca ctaaactttg tcgttaacag tgataatttc ctcttcgctt tgctctgcaa
     4921 gcctcgatat ctctgggttc tcagcttttt cccgcgctga aatttagtcg gtaacaagaa
     4981 cgttagctga cgaagcatct tgataaaaat caaaaataaa tacgcttgta acgccgtgcg
     5041 tattgaatcg acggttgtat ttcatatata actcaaatga taatcatgat cattctcaac
     5101 ttgataagag ttctcatatt atgccaagga tcatcaccag gccgtttctt ttttccccac
     5161 tgacgttatg tatttcagcc gtggcctctg ccgcgaagac cgcggtaaaa cgaaaaaaat
     5221 tgtttacggc agtactggct cttagctggg cttttagcgt aacggctgcc gaacgtatcg
     5281 tggtcgcagg aggatcgctg acggagctaa tctacgcgat gggcgctggc gaacgcgtgg
     5341 ttggtgtcga tgaaacgaca tcttatccac cagaaaccgc caaactgccg catattggct
     5401 actggaaaca actcagcagc gaaggcattt tgtcacttcg cccggatagc gtaattacct
     5461 ggcaggatgc cgggccacaa attgtgctcg accaattacg agcgcagaaa gtcaacgtcg
     5521 tcaccctgcc gcgtgtgcct gccacccttg agcagatgta cgccaacatt cgccaactgg
     5581 caaaaacgct acaggttcct gaacaaggcg aggcgctggt gacacagatc aaccaacgcc
     5641 tggagcgagt acagcaaaac gtggcggcca aaaaagcccc ggttaaagcc atgtttattc
     5701 tctccgcagg cggaagtgcg ccacaggttg ccggtaaagg cagcgtcgcg gacgccattc
     5761 tttcgcttgc cggagcagaa aacgtcgcaa cctaccagca gtacaaaagc tacagtgcag
     5821 aatcactgat tgcggccaat ccagaagtga ttgtcgtaac ctcacaaatg gtcgatggcg
     5881 atattaatcg gctacgttct attgccggaa ttacccacac tgccgcatgg aaaaaccagc
     5941 gcattatcac cgttgatcaa aacctgattc tgggaatggg tccgcgtatt gctgatgtcg
     6001 ttgagtcttt acaccagcag ctttggccgc aataaatgga tttaaatcag acgaatgaac
     6061 gcaaataaca ccctggatct cacgccacat tttgctttag acggtgatca gccatttaaa
     6121 gacaggcgcg ccatgatgcc gtttcgtggg gccattccgg ttgccaaaga gcaactggca
     6181 cagacctggc aagagatgct caatcaaacg gtatcaccac gtaagcgact ggtttatcta
     6241 cacattcctt tttgtgcaac acactgcacg ttttgtggtt tttatcagaa ccgttttaat
     6301 gaagatgcgt gtgctcatta taccgacgcc ctgattcgtg aaatagaaat ggaagcggac
     6361 agcgtattgc atcagtctgc ccccatccac gcggtctatt tcggtggcgg cacgccttcc
     6421 gcgctttctg cacacgattt agccagaatt atcaatatat tacgagaaaa gctgccactg
     6481 gcccctgatt gtgaaatcac tatcgaaggc cgggtactga attttgacgc tgagcgaatc
     6541 gatgcttgtc ttgatgcagg agcaaaccgc ttctcaattg gtatccagtc gtttaacagc
     6601 aaaatccgca agaaaatggc ccgcacctcg gatggcccaa ccgccattgc gtttatggaa
     6661 agcctggtca aacgtgaccg tgctgcggtg gtctgtgacc tgctgtttgg cctgccgggt
     6721 caggatgcgc aaacctgggg agaagatctg gctattgccc gcgatatcgg tctcgacggc
     6781 gtcgatctct atgcgctcaa tgtcctgccc aatacaccgc tgggcaaagc cgtggaaaat
     6841 gggcgtacta ccgtgccctc tccggcagaa cgtcgcgatc tttatctgca agggtgtgat
     6901 tttatggacg atgccggttg gcgctgcatc agtaacagcc actggggccg taccacacgc
     6961 gaacgcaatc tctataacct gctgattaaa caaggtgccg attgtctggc ctttggttcc
     7021 ggagccggcg gttcgattaa tggttactcc tggatgaacg aacgcaatct tcagacctgg
     7081 catgaatccg tcgcggcagg caaaaaaccg ctgatgatga tcatgcgtaa cgccgaacgt
     7141 aatgcgcaat ggcgtcatac cttgcagtca ggtgttgaaa cagcgcgtgt accgctggat
     7201 gaacttacgc cacatgccga aaaactcgcg ccgttactgg ctcaatggca ccaaaaaggc
     7261 ttaagccgcg atgcgtcaac ttgcctgcgg ctgactaatg aaggtcgttt ctgggcaagc
     7321 aatattttgc agtctcttaa tgaactaatt caggtactaa atgcgccagc gattgtgcgt
     7381 gaaaaaccat aacaggagtg atttatgagc catgtctcgt tacaagaatt tttgaaaacg
     7441 gagccggacg gtacgctgga ggtggtagcc gaacaataca acaccacact gctggaagta
     7501 gtgaaaaacc tgccctcctc aaccatagtg tcaggcgata aattcgatac tgtctgggat
     7561 accgtctgcg aatggggcaa cgtcaccacg ttagtacata ctgccgatgt tatcctcgaa
     7621 tttagcggcg aactgccttc gggtttccac cgccacggct atttcaacct acgcggaaag
     7681 cacggcatgt ccgggcatat caaagcagaa aactgcacac atattgcctt gatcgaacgt
     7741 aaatttatgg gcatggacac cgcatccatt ctctttttca acaaagaagg cagcgcaatg
     7801 ctgaaaatct tcctcggcag ggatgaccat cgtcagctcc tgagcgaaca agtcagcgcc
     7861 ttccatgctc tggcagcatc gttaaaggaa cacgcctgat gacgccgtgg ctactctttg
     7921 gcgcaggcgg aaaaggtgtg ggtgccagaa cgcttgaact ggcgctggcg gaacaacgtc
     7981 cggtcgttgc tgtcgttcgt catgctgatg ccgccacgaa actggcgcaa caaggcgtac
     8041 aggtttttac aggcgacgcc tgtgatgcca gcgtagtcgc ggctgcttgc cgcgcagctg
     8101 gccaggatgc gcttatcatc tcaacaatgg gcggaactca ggattatctg gcacacagaa
     8161 cagtgattga tgaggccgaa aaggccggca tcagtcgcat gattctggtg acctctttgg
     8221 gatgtggcga tagctggccg tttttatcgg agcgggcaaa ggctgcgttt ggtcaggcgg
     8281 tacgggaaaa aacgctggcg gaaagctggt tgcaaaccag ccagcttgat tacgccattc
     8341 tccgccctgg cggtctgctt gatggggcgg caacgggcaa agcacaacgg atacaaaatc
     8401 aggagtgtca tggttttgtt caccgtgccg atgtcgccgc acatatccat gaactggcaa
     8461 atgcgccagc acttaaccag caggtctaca gtctgattga accggacctg aaaccggcgt
     8521 gataccccgg cgcggcatgt ccgcgccgta atgtcgtaac ggagttttgc gtgctcaagg
     8581 attcactctc ttccgccagg gtctttatgg ggctatcact attattgcta gctctggtgc
     8641 tgttcggtgc cagtcaggga gcgttaagga tcagttttga tgctcttttt gatgaggaat
     8701 accgtgatat ctggctcaat attcgtctac cacgggtttt gctggcggtg ctggtaggtg
     8761 cagcgttggc aaccgccggg gtaattatgc aggggctttt tcgcaaccca atggctgacc
     8821 caggattact tggcgtcagt agcggttctg cattaatggt tggcgttgct attgtgctgc
     8881 ccttctcctt cccagtggtg ctggtgctct atgagcaaat ggtgtttgcc atcgccggaa
     8941 gtttagtggt ctgtaccatc atttttctca tcacgcagcg ccatcgcgat ggcagcatga
     9001 tgcaattgtt actcgccggt atcgccatca atgccctgtg cggcgcagcg atcggcatcc
     9061 tgagctatat cggcgatgag caacagctac gacaactcac gttgtggatg atgggcaatc
     9121 tcggtcaggc gcaatggcca acgttattgg ttgccagttc cttcattctg cccgccatta
     9181 tggcaacaac gtgtctcgcc ggaacgctga atttgctgca acttggtgat gaagaagccc
     9241 attacctcgg cgtgaacgta aagcgtaaac gccaacaatt actgttgatt agctcactgc
     9301 tcgttggtgc cgccgtatcg gtaagcggca ttatcggctt tattggcctg gtgatcccgc
     9361 atctgattcg tatgactacc ggggcaaatc accgctggct aatcccttgc tccgccctcg
     9421 ccggagcctg tttattgctg atggcagaca cgcttgcccg cacgctggta cagccagcag
     9481 aaatgcccgt gggattatta accagcctgc ttggcggtcc ttattttatg tggttgattc
     9541 tgcgcaaccg gaggatcaca tgatcagcgc ccagaatctg gtttatagcc ttcaggggcg
     9601 gcgtctgact gacaatgtct cgctgacttt tcccggtggg gaaattgttg ccattctcgg
     9661 ccctaatggt gcgggaaaat ctacgctgct tcgccagtta accggctatc ttcaacctga
     9721 tagcggcgaa tgtcggttgt ttaataagcc attaaatgaa tggtcgatta ctgaactggc
     9781 gaaacaccgg gccgtgatgc gtcaaaatag ccacatggcg tttccattca gcgtacaaga
     9841 agttatccag atggggcggc atccgcatcg cactggtaat caggataatg aaacagcaca
     9901 aataatggcg ttatgtgact gtcaggcgtt agcaaaccgt gactatcggc aattatccgg
     9961 tggtgaacaa cagcgcgtgc aattagcgcg cctgctggtg caactctggg aacctactcc
    10021 ctcaccgaaa tggttatttc ttgatgaacc aacttcggcg ctggatattc accatcagca
    10081 gcacttgttt cgtttattac gccagctagt tcacgaacgg cagttcaatg tttgctgcgt
    10141 attgcacgat ctaaatcttg ccgcgcgtta tgcagaccgt gttgtattaa tgcaaaaagg
    10201 aaaggttatc gcaaatggta aaccacagga cgtattgaca cagcaggcgc tgaccatgct
    10261 ttacggcgcg gatattaccg tacttaaaga tcctgccagc cattcacccc tgatcgtcct
    10321 cgatcattaa aaccaacagg agtaccgctc tggtactcct gtgaataaat aactataaag
    10381 attagctaat tgaaacgcct ttcacccctg ccataccttt taataatcgc aacaggtctt
    10441 ctatcgacgt tgtggcatga agctggatat caatggcgac cacttcatga tcttcatttt
    10501 cctgcaacga aactaaatca gtttttattt tttgctgctt aaaccagtcg agcattgata
    10561 ccgtattacc gttcactaat gtcagctgta aatggtaatt tttgttcatc aggcggaagg
    10621 ttaattgatg gaagacttcc agcaccagca aggtcatcac tgaaccataa atgcccagtt
    10681 cgtacatacc gctaccaata accataccaa tggcggcggt cacccagata tccgctgccg
    10741 tcgtcagacc tacaatattt tggttacgaa cgagaatgtt acctgcgccg ataaacccga
    10801 cgcccgtcac cacctgagca gcaatacggc tggggtccag tccgacgtga tttaaagaca
    10861 gcacgtcagc aaaaccatat ttcgaaacaa tcataaacag ggcgcttccc atgccaatta
    10921 atacatgtgt gcgtaaccct gctcctttgc cgcgcatttg cctttccatg ccaatagcgc
    10981 cacaggcaat tgctgcgagt ataagacgta taataaattc tgctgtcata taaaaaagca
    11041 aaaaggagca gcaagatggc tcaacttgct actccttttt acttgtacct cattaattcg
    11101 gcaagtcatt agatgcttga tttttgaagg tatacaaatt tttctgcggg tttttcttgc
    11161 agtattcgat cactttagga atttgagtga gatcggtttc atttaaagta acggtatcgc
    11221 cacctttata tactgtctct tcatgcagca tccaccatgc aaccggtgtc attgcttttg
    11281 gattcagatc aataaattcc tggcaggtca tatctttagc ggattcattg gctgccagcg
    11341 cagattgtgc gttcaccagt gacaaagccg ctacagcgcc cataaaaata aacgctttac
    11401 ggagagatga aatattcatt ttgtaaccca ttcaatatag agattatata tgaacaggag
    11461 gaatagctgc ttttaaagaa gatattccgg cctgttcaat atgcaaggaa gtacgatgta
    11521 acgcattccc ggaattacat atctttctta attttgtccc actcgccttt aactttatct
    11581 ttaaagttgg cttgtttatc ctgagtacaa gcctgaacga tagctggggt tacggttgca
    11641 ataccctgaa catctaaaac cgcatcttct ggtttatctt tgttgttcag cgcttcagca
    11701 aaaccaactg cagttggctg gaaggattcg tccacagcca agaaatcttc acaggtccag
    11761 gagttgaccg gttttttgtt atcggctgct ttttgcgcat ccgctgcatt gctcacaact
    11821 ggcagaagaa gcagaccacc aagaataacg cctaatactt ttttcatcgt aatatcctca
    11881 actataaagt gaaagagccg tcacgaatca atttcgacac tgaggttata acctggtttt
    11941 ctgtatatgt catgttgatg gaaaatatca aaatcagata tttttatttc aatacaatga
    12001 gttacagatg catcagatac tgtaattagg aaatttttat taaatcga
//
LOCUS       DARNVM010000147        11979 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.147, whole genome shotgun sequence.
ACCESSION   DARNVM010000147 DARNVM010000000
VERSION     DARNVM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.147"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            272..595
                     /locus_tag="SI621_003702"
     CDS             272..595
                     /locus_tag="SI621_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEI4066364.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            592..1422
                     /gene="amiD"
                     /locus_tag="SI621_003703"
     CDS             592..1422
                     /gene="amiD"
                     /locus_tag="SI621_003703"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HEI4066365.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1419..2432)
                     /locus_tag="SI621_003704"
     CDS             complement(1419..2432)
                     /locus_tag="SI621_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEI4066366.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPSLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2531..3961)
                     /locus_tag="SI621_003705"
     CDS             complement(2531..3961)
                     /locus_tag="SI621_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEI4066367.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTEL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(3972..4973)
                     /gene="ltaE"
                     /locus_tag="SI621_003706"
     CDS             complement(3972..4973)
                     /gene="ltaE"
                     /locus_tag="SI621_003706"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEI4066368.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5010..6728)
                     /gene="poxB"
                     /locus_tag="SI621_003707"
     CDS             complement(5010..6728)
                     /gene="poxB"
                     /locus_tag="SI621_003707"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEI4066369.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATSHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6861..7829)
                     /gene="hcr"
                     /locus_tag="SI621_003708"
     CDS             complement(6861..7829)
                     /gene="hcr"
                     /locus_tag="SI621_003708"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HEI4066370.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7841..9493)
                     /gene="hcp"
                     /locus_tag="SI621_003709"
                     /gene_synonym="priS"
     CDS             complement(7841..9493)
                     /gene="hcp"
                     /locus_tag="SI621_003709"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HEI4066371.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9637..10536)
                     /gene="lysO"
                     /locus_tag="SI621_003710"
     CDS             complement(9637..10536)
                     /gene="lysO"
                     /locus_tag="SI621_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HEI4066372.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITIILLCNIIALLWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11020..11715)
                     /gene="aqpZ"
                     /locus_tag="SI621_003711"
     CDS             complement(11020..11715)
                     /gene="aqpZ"
                     /locus_tag="SI621_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEI4066373.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGDYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT         2938 a         3198 c         3177 g         2666 t
ORIGIN      
        1 gcaagatggg gaagacttta ggaatcattt acaatataag ttgactcttc ccactcatta
       61 atgttgctgg tactacataa tacgacaggt aagcggtgct ccataataaa actaatatcg
      121 ggttatgcaa cggataattg tacaggctta tgtacctgtt tttgacacaa tgagatgatt
      181 tcctaaactg atcaaaacct tcttcccaac atggcatatc agtccgataa tgctttagag
      241 atcgaaggct taacaaacaa ggagatcgat catgcaattt tctacaaccc caactctgga
      301 aggccagacc atcgtcgaat attgcggtgt ggtgaccggc gaagcgattt taggtgccaa
      361 tattttccgt gatttctttg ccggtatccg cgatatcgtt ggcggacgct ccggggctta
      421 tgaaaaagaa ttgcgtaaag cgcgggagat tgcctttgag gagctcggcg atcaggcccg
      481 ggcgctgggg gctgatgccg tagtcggtat tgatatcgac tacgaaacgg tcgggcaaaa
      541 tggcagtatg ctgatggtta gcgtcagcgg tacggcggtg aaaacgcgtc gatgagaaga
      601 gtcttctggc tggtcgccgc cgctctgtta ctggcagggt gtgccggcga aaagggcatt
      661 gtcgagaaag agggatatca gcttgatacc cgacgccagg cgcaggcggc gtatccgcgc
      721 attaaagtgc tggttatcca ctacaccgca gatgattttg atagctcgct ggcgacactg
      781 accgataagc aggtcagttc gcattatctg gtgcctgctg tgccaccgcg atacaacggt
      841 aaaccgcgca tctggcaact ggtgccggaa caggagctgg cctggcatgc ggggattagc
      901 gcctggcgcg gggcaacgcg ccttaacgac acctctattg gcattgagct ggaaaaccgt
      961 ggctggcaga aatcatccgg agtgaaatat tttgccccgt ttgaacaggc acagattcag
     1021 gcgcttattc cgctggcgaa agatattatt gcccgttatc acatcaagcc ggaaaacgta
     1081 gtggcgcatg cggatattgc gccgcagcgc aaagacgatc cggggccgtt attcccctgg
     1141 caacaactgg cgcagcaggg gattggtgcc tggccggatg cgcaacgggt gaacttttac
     1201 cttgccgggc gcgcgccgca cacaccggta gataccgcat cattgctgga gcttttggcg
     1261 cgctacggtt atgacgttaa acctgatatg acaccgcgcg agcagcggcg cgtgattatg
     1321 gcgttccaga tgcatttccg cccgacgtta tacaacggcg aagcggatgc agaaactcag
     1381 gcgattgccg aagcgttgct ggagaaatac gggcaggatt agcgcggcag ttttccgtgg
     1441 tcgcgtagcc aggcggcagt tttctcgata ccttcatcca gggtgaggac tggttgataa
     1501 cctaactctt cctgcgcacg cgtaatatcc agcgtaaagt caaaattcaa cttggagacg
     1561 ccgtagtggg tcagcgacgg ctcttttgct gacttgcggc ctaaacgctc catgctgcgg
     1621 gcgatcatat ccagcatcgg gtaggggacg gaacgaatac gacagtcaat attcaactcg
     1681 tcgatcagct tctgcacgat gctgcgcagt gtgcgatgct cgccgttggt gatgttgtac
     1741 acacgcccgg aaggtagctt atcgcaggct tcctggcttg ccagccacat ggcgtgcacg
     1801 gcattttcat agtaggtcat atcgaccagc gcactgccgc cgtgcggtaa cagaatgctg
     1861 ccgtagtggt gcatcatatg cgccagacgg gggataaaga ctttatcgtg cggtccgaac
     1921 agactttgtg ggcgcagaat agtaaagcgc gtttgtggat tcgcctgcga aagcatattg
     1981 atcacttctt cgctggctgc tttgctgcgg gcaaactcgt tggcaaagcg gtgagggcga
     2041 aaatcttctt taatatcgcg atggtggtga taatcgaagt acagggaggg ggaagagata
     2101 tgaataaagt tacgcacacc ccaggcgaca gcccattcac ccaggcggcg agtggcgcga
     2161 acgttagcca gatcgaaagc ctgttgtgtc ccccagggcg aggtaaagct ggagcagtgc
     2221 cacagcgtat caatgcccgc gagcatcact ttagcttgtg atgagaccag ctcggtcaga
     2281 tccgccggaa caaactctgc gcccattttt tccagcaatt tacccattgc ctcgttgcga
     2341 ccggtcgctc gcacgctgat gcctttctgg cataaaaact ctaccgcgtt tcgacctaag
     2401 ccgctggtgg cgccggtaac cagtaccttc atatcaatcc actgttgttg agaaaatgac
     2461 gtgcgcattc ttccgtgatt tcccccatga tgcaatggga aacatgaaag aataacgcag
     2521 gttttgtcga ttaatctgtg ctttgttctg ccagtctggc gattcgtttt gccattccgc
     2581 gaaaaataaa cagatgcgcg gggatcatca ataaccagta aaacagcccc ggcataccgt
     2641 gcggatgcca gaatgcgcga acatcgatag tacgatagtc gcctttatct tccagggtaa
     2701 aacacagtcg tcccagcccc ggcgctttca tgccaaataa cagcgtaagt tgtttttccg
     2761 gttcaacgac aatcactttc cagctatcca ccgcatcgcc ggtctgcaaa tattcacgct
     2821 ccgggcggcc tttcgccagc ttatgaccga tcgcgcggtc catcaacgcc cgggtttgcc
     2881 acaaaatatt gccaaagaaa taacgctctt taccgccgat ttgattcacc acctgccata
     2941 aagccgcaag gctggcggac gttttaacgg taaaccccgc ctgtttggcg aaataaccgt
     3001 actccggtcg ccagcgggca aaggcctgag cgtcgtagcc ccagtcgctg gagttgacca
     3061 gcttttcttc ctctttcagg gtgcgacgta ccgcgtcatc gaaagcgatc agccgttgtg
     3121 ggatgagcgc acgtagctcg gtgtcatccg ccagcagatc gtgtttcagc ccctgaatca
     3181 acgccctggc ggtggtgggt ggtacggaag taatcacatt gagaaaccac accgaaatcc
     3241 agcgggtggg gagagggatg gggatcaacc agcgacgctt accgctcacc gccataaaat
     3301 gttcaaactg ttgctgataa ctgagcactt ctggtccggc ggcttcgaag atgcgatgtt
     3361 cgcaggccgg atgatctaac agcgccacca gatagtgcag caagttttcc agcgcgatgg
     3421 gcgtggtgcg tgaacgtacc cagcgtggcg gcgttaagac tggcaggttg tagaccatat
     3481 cgcgcatgac ttcgaacgcc gctgaacctg caccaacgat tattccggca cgcaattcgg
     3541 tcaccgggac acccgcttca cgaagaatat ccgccgtagc ctgacgggca cgcagatggt
     3601 ccgattgctc atgtggcggg gcctgcaacg aactgagaaa gattaattgc ttaactggga
     3661 cttcacgtag cgcatcgcgg acgttgagag ccacctggcg ctcctgagcg ataaaatcgc
     3721 cgccttcgcc catgctgtgc accagaaaat agaccgtatc gatatcctgc aacagggccg
     3781 gaaggttatc cggccagctg agatcgactt tatggcaact gacatttgcc agttgcagct
     3841 ttgcaagcct gtcgacatga cgtgccgccg ccaggatctg atgcccctgc tggctgagtg
     3901 ttcgcaccag atgctgacca atgtagccac tggcaccgag aactaaaatg cgttgcggca
     3961 cgtctccctc cttaacgcgc caggaatgcg cgccagtggg cggcgacttc agccagttgc
     4021 tcgcgcgaga cgtcgagatg cgtcaccagg cggacaatcg gcgaggcgtt aatcagcacg
     4081 tttctcgctt tcatgtattc gcctaacgcg gcagcatttt cttccccgac gcgaacaaac
     4141 agcatattgg tgtcctgacg catcacatcc gcgcctgctt cacgcaactg ctccgccatc
     4201 caggcggcgt tgtcgtggtc ttcctgtaag cgcgcgacgt tatttttcag ggcatacatc
     4261 ccggcggcag ccagaatgcc ggactggcgc atcccgccac cggtcatttt ccgccagcgg
     4321 atggcacgtt taatgtaatc acgattaccg accagtaacg agccgactgg cgtcccaagt
     4381 cctttcgaca ggcaaatggt gaacgaatca caatattgcg tgatctcttt cagttcgcag
     4441 ccgtaagcca ccacggcatt aaagatgcgc gcaccgtcaa catgcagcgc cagattgcgc
     4501 tcgcgagtaa attcccatgc ttctttcagg tattcccgcg gcagcacttt gccgttgtgg
     4561 gtgttttcca gactgagtaa ttttgtgcgg gcgaaatgga tatcgtcggg tttgattttc
     4621 atcgccactt tatccagcgg tagcgtgccg tcggcagccg cgtcgatggg ttgcggttga
     4681 atactgccca gcaccgccgc gccaccggct tcaaacagat agttatgcgc ggcctgaccg
     4741 acaatatact cttcgccgcg ttcacagtga ctgagcagag cgaccaggtt ggcctgagtg
     4801 ccggtaggca gaaaaatggc ggcttcttta ccggaaagct ctgctgcata gtcctgcaga
     4861 gcattaacgg tagggtcgtc tccgtaaacg tcgtccccaa ccggggcggc catcatcgct
     4921 tcgagcatgg cgcggctcgg tcgggtaacg gtatcactgc gtaaatcaat catggcatgt
     4981 ccttattatg acgggaaatg ccaccctttt taccttagcc agtttgtttt cgccagttcg
     5041 atcacttcat caccgcgtcc gctgatgatt gcgcgcaaca tatacaggct aaaacctttg
     5101 gcctgttcga gtttgatctg cggtggaatg gctaattctt ctttggcgac caccacatcc
     5161 accagcaccg gaccgtcgat ggaaaaggcg cgttgcaggg cttcatcgac ttcagaggct
     5221 ttttccacgc gaatacccgt aattccgcac gcttcggcaa tgcgggcaaa gttggtgtca
     5281 tgtagttcgg tgccgtcagt caaatagcca ccagctttca tctccatcgc cacaaagccc
     5341 agcacgctgt tattaaagac gataattttc accggcagtt tcatctgcac tactgagagg
     5401 aaatcgccca tcaacatgct aaaaccgcca tcgccgcaca tggcgaccac ctgacgttcc
     5461 ggctcggttg cctgagcacc cagcgcctgc ggcatggcgt tagccatcga accgtggtta
     5521 aacgaaccta agagacgacg cttgccgttc attttcagat aacgtgccgc ccacaccgtt
     5581 ggcgtaccaa cgtcacaggt aaagatggcg tcatcggcgg caaaatgact aatttgctgc
     5641 gccagatatt gcgggtgaat ggctttctcg ctcggtttag ctaaatcgtc cagccctttg
     5701 cgggcgtcgc ggtaatcttc cagcgcttta tccagaaact tgcgatcggc tttttcttcc
     5761 accaatggca gcaatgcacg cagagttgac ttgatatcgc cgaccagtgc catatccacc
     5821 ttgctgtgag cgccgatgct ggctgggttg atatcaatct gaatgatttt ggcatcggtc
     5881 gggtagaagg cgcggtaggg gaactgagta ccgagtagca ctaacgtgtc ggcgttcatc
     5941 atggtatgga aacctgacga gaagccaatt aatcccgtca ttccgacatc atacggatta
     6001 tcgtattcga catgttcttt accgcgcagg gcatgaacaa taggcgcttt aattttcccg
     6061 gcaaactcaa ctaactcttt atgcgccccc gcgcagccgc tgccacacat cagagcgata
     6121 ttgctggaat aacgcagcag ttgcgccagt ttgcgtaact cttcttcttc cggcgtcacg
     6181 actggctgtg gcgcatgata ccagtgcgag gttgcccctt ctggcgcagg ttttaacgcc
     6241 acgtcgcccg gtaacacgac aacggaaacg ccacggttta gcaccgcttt gcgcatggca
     6301 atcgccagca cttgtgggat ttgctccggg ctggaaacca gctcgcaata gtgactacat
     6361 tcgcggaata actcttgtgg gtgggtttcc tggaaatagc cgctgccaat ttcgctggag
     6421 ggaatatgag cggcaatcgc cagtaccgga acgtgattgc ggtggcaatc gaacaggccg
     6481 ttgattaagt gcaggttgcc ggggccgcac gatccggcac agaccgccag ttctcctgaa
     6541 agttgtgctt cagcaccagc ggcaaaggcc gccacttctt catggcgggt ggacatccac
     6601 tcgatggtgc ccatgcgatt aagactgtcg ctaagaccgt tcagagagtc gcccgtgact
     6661 ccccagatgc gtttcacgcc tgccgattcg agtgttttgg cgatataagc tgcaaccgtt
     6721 tgtttcatgg ttctccatct cctgaatgtg ataacggtta caagtttagt tcatctgacg
     6781 gagggggaag ggatgggaga aaaaggaggc actaacggtt aaatagcccg atgaaaggaa
     6841 tatcatcggg cataaggcga ttatgcgaga accaaatctc cctgcggatg gcaggagcag
     6901 gccagcacgt aaccttcagc gatttcggcg tcggtcagcg tcattgtgct gctcaccgta
     6961 tattcaccgg aaaccacttt cgtcttacag cagccgcaaa cacccgcacg gcaggcagcg
     7021 acaaccggaa cgttattgct ttccagcgcc tccagtagcg tggtgccaac cggggcgtaa
     7081 aattctcgtg ccggttgcag tttggtgaat ttcagaccgc tggtcgccgc ttccgctact
     7141 ggggtgaaga atttctcttt aaagaaacgc gtcacgccga gcgctttcac ttcctgctct
     7201 acccaatcca tatacggagc cgggccgcag gtcatcacgg tacgtgaagc taagtcagga
     7261 acgcgagtca gcagttcgcg agtgagacga ccagcgataa agccttcggt aacgttattt
     7321 tctgccacca gcgtcaccgg atagttacgc cactcatcgg cgaaaataac atcctgcggc
     7381 gaacgcacgt tgtagatcac ctgcacatcg gcctgcggac ggttcttcgc cagccagcga
     7441 cgcatcgaca taatcggcgt gacgccgcag cctgccgcca gcaacaggaa tttatcttct
     7501 gctttatcgt cgcaggtaaa ttcccccatc gcgtccgaaa gccagagata atcaccgcgt
     7561 tttacatcgc gcgtcagcca ctgggagccg acaccgtcat caatccgccg cacagtcagg
     7621 gtgatgtatt cactcacgcc tggcgtggag gaaatggtgt aagcacgcag cgtttcggct
     7681 gagttacgca cgctgaccag tgcatattgc ccggcgcgat atgggtagta atcgtggcaa
     7741 atcagggaaa tcgtccacac atccggcgtt tcttgcttaa tgtgatgaac ctgcatccgc
     7801 cacgggcatt gattcgttgg catcgtcatc gacaaactcc ttacgcgctc aacagttgct
     7861 tcatgtcttc ttcaacagtg gtgatagaac gcaggccgaa tttctcgttc agcaccgcca
     7921 gcaggtctgg tgtcaggaaa ccaggtgcag tcgggccggt gacgatattt ttcacaccca
     7981 gagaaagcag tgtcagcaga atgacaatcg ctttctgttc aaaccacgag agcaccagcg
     8041 acagcggcag atcgttgact ccgcagccca gtttctctgc cagcgttacc gccagaataa
     8101 tcgctgagta agcatcgtta cactgaccag catctaccag acgcggcaga ccttcgatat
     8161 cgccaaactc gagtttgtta aagcgatatt taccacaggc gagagtcagg atcagacagt
     8221 catccggcac gctggtggcg aaatcggtga agtagtgacg ctcgccgcgt gcgccgtcac
     8281 agccgccaag caggaagata tgacgcagtt tttcacgact taccagatca atcagcgtat
     8341 ccgctgcgcc aagcagtgtc tggcgaccaa agcccacggt gatcaggtgc ggaatttcgc
     8401 tgtacgggaa gcccgccatt tgttgcgcct gggcgatgac cgcagagaaa tcttcgccat
     8461 ccagatgacg cacacccggc cagccaacga tgctgcgggt ccagatacga tcgtcataag
     8521 cgcctacggt tgggtcgatg atgcagttcg aggtcatcac gatggggcca gggaaacgag
     8581 cgaactccac ttgctgattc tgccagccgc tgccgtagtt accgaccaga tgcttgaatt
     8641 tacgcagctc cgggtagcca tgcgcaggca gcatttcgcc gtgggtgtag acattaacgc
     8701 ccgtgccttc ggtctgttcc agcaggttgt agagatcttt gagatcgtga ccggaaatca
     8761 gaatgcattt acctgccgtc gctttgacgt tgacctgggt tggggtcggg tgaccgtatt
     8821 taccggtttc gcctgcgtcc agaatgctca tcactttgaa gttcatctgg ccgatttcca
     8881 ttgaacactc aagaagcgcg ttcatatcgg caggccaggt tcccagccac gccatgattt
     8941 tatggtactg ggcgtaaata tcgttgtcgt attgaccgag aacatgcgcg tgttccatat
     9001 aggccgccgc acctttcagg ccatacaggc acagcagacg caggccgaga atgttttcgc
     9061 caatcgccgc tttatctttg ttaggggtaa attctgctgc ctgacgttgc agctcgccga
     9121 gatcatcgct caccagttgc aggtcagcca tcgggttatc gacgcgggcg ttggcatcta
     9181 cagccaggca ttgtgctttc agcgcctcgc gcagggcaat cgcttcacga gcgtagccga
     9241 ctatacgcgg agaatcgaag ttaacgttgg tcagggttga gaaaaatgcg cgtggcgcaa
     9301 agctgtctac atcgtggttg atgatgccgt attcacgcgc ttttaccgcc caggccgaaa
     9361 gcccttgcag cgccgcgatg agtaaatcct gaaggtcaga ggtttccgcc gttttaccac
     9421 acatcccctg cgcgtatgag cagccgtttc ctgccggagt acggatagtt tgttcacatt
     9481 gcacacaaaa catgatcaca ccttttaaag ttatatttaa tatacatgtt taaggttaag
     9541 acgcttaacg cagggataaa agggattttt catgcaactt taagggagat tgatttagcg
     9601 caattttggc ggcagggatc taccgccaga gaggtattac gcagagaaaa aggcgatgag
     9661 gatcggcact aacaggctaa gaataaaacc gtgaacaatt gccgccggga ccatatccag
     9721 cccgccagta cgttgaagaa cgggcagggt gaaatccatc gacgtcgcac cgcataagcc
     9781 cagtgcggta gagcggctgc ggcgaatcag cccagggatc aacataattg caatcagctc
     9841 acgggccaga tcattaaaaa acgccgcgct cccgattacc ggaccaaaag attcagtcaa
     9901 taaaataccg gaaagagaat accagccgaa accagaggcc attgccagcg cggtattgat
     9961 gggaagatca agaataaagg cgttaattaa accaccaatt aatgaactgg caaccaccac
    10021 cacggcgaca atcattcccc ggcgattaag gacaatctgc tttaaggtca tgccattatt
    10081 gcgcaactga ataccgacga ggaaaagtag cagaattaag gtgtattcac tggcttcggt
    10141 cgcatgttgt aagaaagcca gtccactcag gccaatggca aaaccaatca ctactacgcc
    10201 gcacagtttt agcgactcca gcgccatcgc aatacgcgac gggagttttt cttgctggtg
    10261 gtggttgcgc cacggcagac cgcgctccag ccacaacaac gcaataatat tgcagagtaa
    10321 aataatggta atgctgacgg cggaataatg cagaatcgcc aacaggttac tggcgaggtt
    10381 atcgagaaac gccagactga tacccataaa aaagagaatg aggtaaacca tccagcttaa
    10441 tagctgatta ataactctta atgcagcttt ttggcgaaga ggaatgaggt aacccacaat
    10501 caggggaacc agaatgatta acagcccaga aaacatgaaa acccagtcct tgcaaagatg
    10561 aagtcgaaat gcgcgatgac acactactga aagcggaagg acgagtaaag ttgcaattaa
    10621 aaggaaatgt tatgcataag gatcggtagg gtattcattt tcatttaaag atattcttgc
    10681 gctttaatta caaaccgcac cggtgttggt ggcgtcaaaa tcgccgaggc gttccctgaa
    10741 ggccggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat gttacctcgc
    10801 gcccgacccg gtagccgtaa gggataagtc gagggcaccg cgcagcggcg attttgttcg
    10861 ccagagcccg ggggtgcagg gggcggcggc gattggccgc cccctgcgcg ctccctgcgc
    10921 cagtgactat atgtggctta acacatcaag gagcgcaaca ggaagatgaa tcaatatatt
    10981 taaaaaaggc ctgacattgc gccaggcctt ctgcgttaat taattacgct tttccagtag
    11041 agtccggtaa atcagaccac cgataatacc gccgacaatt ggcaccaccc agaagaacca
    11101 cagttgttct aacgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt
    11161 aacagaagtg ttagtcaccg gaatactgat taagtgaatc agggttaagg ccagaccaat
    11221 agcgatcggc gcaaaaccag caggagcgaa tttgtcggtt gcgccgtgga tcaccagcag
    11281 gaaacctgca ctcaatacca gttcaactac cagcgcggaa agcatggaat aatcgcctgg
    11341 cgaatgctcg ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt
    11401 accactggca attaaataca gcagcgccgc cgcaacaata ccgccgacaa cctgggcaat
    11461 tacgtagcca acgacttctt ttgccgggaa acgtccgcca gcccataaac caatagtgac
    11521 cgccgggtta aaatgaccac cagaaatatg accaacagca aaggccatcg tcagaacggt
    11581 cagaccgaat gccagcgcca cgccggcaaa accaatgcct aattccggga agcctgcggc
    11641 cagtacggca ctaccacagc caccaaaaac aagccagaaa gtaccaaaac attcagctgc
    11701 taattttctg aacatatcca cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt
    11761 tatagggtcg tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt aaaaacgaag
    11821 gcgagtcatt caccagataa ataaatccag taaatttgat ttagggcaac agcgggttgc
    11881 cccatatagt catttgtctg attgacagtg tagtgcaagc aaaagattta atcctttagg
    11941 cgtaataaaa aataatttat catgctaatt atttgattt
//
LOCUS       DARNVM010000148        11962 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.148, whole genome shotgun sequence.
ACCESSION   DARNVM010000148 DARNVM010000000
VERSION     DARNVM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.148"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..279
                     /locus_tag="SI621_003712"
     CDS             <1..279
                     /locus_tag="SI621_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEI4066374.1"
                     /translation="VHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDIIFYHD
                     SEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            397..870
                     /gene="yiaM"
                     /locus_tag="SI621_003713"
     CDS             397..870
                     /gene="yiaM"
                     /locus_tag="SI621_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HEI4066375.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            873..2150
                     /gene="yiaN"
                     /locus_tag="SI621_003714"
     CDS             873..2150
                     /gene="yiaN"
                     /locus_tag="SI621_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HEI4066376.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            2163..3149
                     /gene="yiaO"
                     /locus_tag="SI621_003715"
     CDS             2163..3149
                     /gene="yiaO"
                     /locus_tag="SI621_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HEI4066377.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSMAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKLLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            3153..4649
                     /gene="lyxK"
                     /locus_tag="SI621_003716"
     CDS             3153..4649
                     /gene="lyxK"
                     /locus_tag="SI621_003716"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HEI4066378.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRPLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            4646..5308
                     /gene="ulaD"
                     /locus_tag="SI621_003717"
     CDS             4646..5308
                     /gene="ulaD"
                     /locus_tag="SI621_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEI4066379.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVMLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFTAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            5301..6161
                     /locus_tag="SI621_003718"
     CDS             5301..6161
                     /locus_tag="SI621_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEI4066380.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTRPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            6155..6850
                     /gene="araD"
                     /locus_tag="SI621_003719"
     CDS             6155..6850
                     /gene="araD"
                     /locus_tag="SI621_003719"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEI4066381.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRCYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETLEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6882..7697)
                     /locus_tag="SI621_003720"
     CDS             complement(6882..7697)
                     /locus_tag="SI621_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4066382.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            7923..9323
                     /locus_tag="SI621_003721"
     CDS             7923..9323
                     /locus_tag="SI621_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066383.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            9334..11304
                     /locus_tag="SI621_003722"
     CDS             9334..11304
                     /locus_tag="SI621_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HEI4066384.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(11434..>11962)
                     /locus_tag="SI621_003723"
     CDS             complement(11434..>11962)
                     /locus_tag="SI621_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEI4066385.1"
                     /translation="EKNELSLTASWMPLEFDPADNDDYAMQQLDKRDSTAMAGVAWYH
                     HERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENFSDYYYG
                     ISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEITDSPMVD
                     RNESFTFVTGVSWRF"
BASE COUNT         2714 a         3183 c         3183 g         2882 t
ORIGIN      
        1 gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg gtgaagagaa aatcggcatt
       61 gctattgata cgggaaataa taaagtcagc gaatcactat tagagcagcg cgatattatt
      121 ttttatcacg atagtgaaca tgaatcattt atagaaatga taccgggcag ctacgctatt
      181 ttcttcccgc aggatgttca tcgaccaggt tgtattctgc aaacagcctc tgagatacga
      241 aaaattgtgg ttaaagtcgc attaacagcg ctgaattaat cgaataatcg tctacgccag
      301 aacgcctggg taatgttatt gctctaacta tattaataat ttaggttgct gcacatgcaa
      361 cttgaattat gacagacata gcctcaagga atagctatga aaaaaatact tgaagcaata
      421 ctggcgatta atctcgccgt actttcctgt attgtgttta taaacattat tttaagatat
      481 ggttttcaga caagcatttt atctgtagat gaattgtcac gttatttatt tgtctggtta
      541 acatttattg gcgcgattgt ggcttttatg gataacgccc acgttcaggt cacctttctg
      601 gtggagaaac tctcccccgc atggcagcga cgagtcgctc tggttaccca ttccttaatc
      661 ttatttattt gtggcgcact ggcctggggc gcaacgctaa aaacaattca ggactggagc
      721 gattattcac cgatcctcgg tttgcctatc ggcctgatgt atgccgcttg tctgcccacc
      781 agcctcgtta tcgcattctt tgaactgcgt catttatatc aactcatcac gcgtagcaat
      841 tctctaacgt cgccaccgca aggagcttaa tcatggctgt gctgattttt ctgggctgtc
      901 tgttgggtgg tattgctatc ggtttgccta ttgcctgggc actgttgttg tgcggagcgg
      961 cgttaatgtt ctggctggac atgtttgatg tccagatcat ggcacaaacg ctggtgaacg
     1021 gagccgacag cttctccctg ctggcgattc cgttctttgt gctggcgggt gaaatcatga
     1081 atgcgggcgg cttgtcaaaa cgcattgttg acctgccgat gaaactggtg gggcataaac
     1141 caggcgggct gggctacgtc ggcgtgctgg cggctatgat tatggcaagc ctttctggtt
     1201 ctgccgttgc ggataccgcc gccgttgccg ctctactggt gccgatgatg cgcagcgcca
     1261 actacccggt caaccgggcg gcggggctga ttgcctctgg cggcattatc gcgccaatta
     1321 ttcccccttc cattccgttt attattttcg gcgtttccag cggattatcc atcagtaagc
     1381 tgtttatggc gggcattgcc cctggcatga tgatgggcgc aacgctgatg cttacctggt
     1441 ggtggcaggc cagtcgcctt aatttacccc gccagcaaaa agcaacgatg caggaaatct
     1501 ggcactcttt tgtctccggt atctgggcgc tgtttcttcc agtcatcatt attggcgggt
     1561 ttcgctccgg tctgtttacc cctaccgaag ccggggccgt tgctgctttc tatgcgctgt
     1621 ttgtcgccac agttatttac cgtgaaatga ccttcgccac actttggcat gtgctcattg
     1681 gcgcagcgaa aacgacctca gtggtgatgt ttctggtggc ctcggcacaa gtttccgcct
     1741 ggctgatcac cattgctgaa ctgccgatga tggtttctga tttactgcaa ccgctggtcg
     1801 attcaccgcg tctgctgttt atcgtcatta tggtggctat cctgattgtc ggcatggtca
     1861 tggatttaac gccgacggta ttaattctta ccccggtatt aatgccttta gttaaagagg
     1921 caggaatcga tccgatttat ttcggtgtca tgtttatcat taactgctca atcggcttaa
     1981 ttacaccgcc tatcggtaat gtgcttaacg ttatttcagg ggtggcaaaa ctcaaattcg
     2041 atgatgcagt cagaggcgtt ttcccttacg tcctggtttt atattcatta ttagtcgtat
     2101 ttgtttttat tcccgatctc atcatcctcc ctttgaaatg gattaattaa aaggaaaata
     2161 ttatgaaatt acgctctgta acctacgcat tattcattgc tggcctggct gcatttagta
     2221 catcttcaat ggcggcgcag tctttacgtt tcggttatga aacctcacaa accgactcgc
     2281 aacatattgc ggcgaaaaaa ttcaatgatt tattgcagga gagaaccaaa ggcgagctga
     2341 aattaaaact gttcccggac agcactctcg gtaacgcgca ggcgatgatc agcggcgtac
     2401 gtggcggcac catcgatatg gaaatgtccg gctcgaataa ctttgctggg ttatcaccag
     2461 tgatgaactt gcttgatgtc cctttcctgt tccgcgatac cgcccacgcg cataaaacgc
     2521 tcgacggcaa agtcggtgat gatttgaaag cctcacttga aggtaaagga ctgaaattac
     2581 ttgcctactg ggaaaacggc tggcgcgatg tcaccaactc ccgcgcacca gttaaaaccc
     2641 ccgcagacct gaaagggttg aaaatccgca ccaacaatag cccgatgaat atcgccgcat
     2701 tcaaagtctt tggcgctaac ccgatcccga tgccgtttgc cgaagtctat accgggctgg
     2761 aaacgcgcac tatcgacgct caggaacacc cgatcaacgt cgtctggtca gcaaaatttt
     2821 tcgaagtaca gaagtacctt tctctgacac accacgccta ttcgccactt ctggtggtga
     2881 tcaacaaagc gaagtttgac ggcttaaccc cggagttcca gcaggcgctg atctcatctg
     2941 cgcaggaagc gggtaactac cagcgcaaac tggttgccga agatcagcaa aaaatcatcg
     3001 acggcatgaa agaagcgggc gtggaagtca tcaccgatct cgaccgcaaa gcctttagcg
     3061 acgcactggg gaatcaggtt cgcgacatgt ttgttaaaga tgtgccgcag ggagctgatc
     3121 tgctgaaagc cgtggatgag gtgcaataaa ccatgacgca atactggctg gggttagatt
     3181 gtggcggtag ctggctgaaa gccgggctgt atgaccgcga aggccgggag gcaggcgtgc
     3241 aacgcctgcc gctgtgcgca ttaagcccgc agccaggctg ggcagagcgc gatatggcag
     3301 aactgtggca atgctgcatg gctgtcattc gccccctgct tactcattct ggtgttagcg
     3361 gggaacaaat agtcggtatc ggcatctccg cacagggaaa gggcttgttt ttgctggata
     3421 aaaacgacaa gccgctcggg aatgctattt tgtcctcgga ccgccgggcg atggaaatcg
     3481 ttcgtcgctg gcaggaagat ggcatcccgg aaaaactcta cccgctgacc cgacaaacct
     3541 tgtggaccgg gcatccggtg tcgctgttac gctggctgaa agagcacgaa ccagaacgct
     3601 acgcgcaaat tggctgcgtg atgatgacgc acgactacct gcgctggtgt ttaactggcg
     3661 tcaaaggctg tgaagagagc aatatttccg agtccaatct ctacaacatg agtcttgggg
     3721 aatatgaccc gtgtctcacc gactggctgg ggatcgctga aatcaaccac gccctgccgc
     3781 ctgttgtcgg atctgccgaa atctgcgggg aaatcaccgc tcagacagcc gcactgaccg
     3841 gtctgaaagc gggtacgccc gttgtcggcg gcctgtttga tgtggtttcc accgcactct
     3901 gtgccgggat cgaagacgaa tttaccctta atgcggtgat gggcacctgg gcggtgacca
     3961 gcggaataac ccgtggttta cgtgacggtg aagcgcatcc gtatgtctat ggtcgctacg
     4021 ttaacgatgg tcaatttatc gttcacgaag ccagccccac ctcttccggc aacctcgaat
     4081 ggtttaccgc acagtgggga gaaatctcgt ttgatgagat taaccaggcc gttgccagct
     4141 tgccgaaggc cgggggcgat ctctttttcc tgccgttcct gtacggcagc aacgccggac
     4201 tcgagatgac cagcggtttc tacgggatgc aggccattca cacccgcgca cacctgttgc
     4261 aggccattta tgaaggcgtg gtgttcagcc atatgaccca tctcaaccga atgcgcgaac
     4321 gttttactga tgtgcacacc ctgcgcgtca ctggcggccc ggcgcattcc gatgtctgga
     4381 tgcaaatgct ggcggacgtc agcgggctgc gtatcgaact gccgcaggtg gaagaaaccg
     4441 gctgttttgg cgcggcgctt gccgcccgcg tcggcaccgg ggtttatcgc gatttcagcg
     4501 aagcccaacg tgatttacag cacccggtac gcaccctgct gccggatatg accgcccatc
     4561 agctttacca acaaaaatac caacgctatc agcatctcat tgccgcactt cagggctttc
     4621 acgcccgcat caaggagcac acattatgag ccgaccactt ctgcaactgg ccctcgacca
     4681 ctcatcactt gaagacgcac agcgcgacgt gatgctgtta aaagacagcg tcgatatcgt
     4741 cgaagcgggc accattctct gtttaaacga agggcttggc gcagtgaaag ccttgcgcga
     4801 acagtgcccg aacaaaatca tcgtcgctga ctggaaggtc gccgatgccg gtgaaacgct
     4861 cgctcaacaa gcatttaccg caggtgctaa ctggatgacc atcatctgcg ccgcaccgct
     4921 cgcgacggtg gaaaaaggcc acgcgatggc gcaacgctgc ggcggtgaaa ttcagataga
     4981 gctgttcggt aactggacgc tggacgacgc ccgcgactgg catcgtattg gcgtgcggca
     5041 ggcgatttat catcgcggtc gcgacgcgca agccagcggg caacagtggg gcgaagccga
     5101 tcttgcacgc atgaaggcgc tttccgatat cggccttgag ctttccatta ccggcggtat
     5161 tactcctgct gatctgccac tgtttaaaga tatccgcgta aaggcgttta ttgccggacg
     5221 ggcgctggca ggcgcagcaa atccggtgca ggtggctggc gatttccatg cgcaaatcga
     5281 cgctatctgg ggaggcgcgc gtgcgtaatc atccgttagg gatttatgaa aaagcgctgg
     5341 cgaaagatct ctcctggccg gagcggctgg tgctggcaaa aagctgcggc tttgattttg
     5401 tcgaaatgtc ggtggatgaa actgacgagc gtctgtcacg tcttgactgg agcgccgcac
     5461 aacgtgcttc gctggtcact gcgatgatcg aaacgggtgt tgctattccg tcaatgtgcc
     5521 tgtccgcaca tcgtcgtttt ccttttggta gccgggacga cgccgtgcgc cagcgggcgc
     5581 gggagatcat gagtaaagcg attcgcctgg cgcgcgatct cggcattcgt actattcaac
     5641 tggcaggtta cgacgtctat tacgaggacc atgacgaagg cacccagcaa cggtttgctg
     5701 aagggctggc gtgggcggtt gaacaggcag cggcatcaca agtcatgctg gcagtggaga
     5761 ttatggacac cgcgtttatg aactccatca gcaaatggaa aaaatgggac gagatgctcg
     5821 cctcgccgtg gttcaccgtc tacccggacg tcggcaacct cagcgcctgg ggcaatgatg
     5881 ttcccgccga actgaaactg ggcattgacc gtattgccgc aatccacctg aaagacaccc
     5941 ggccagtcac cgggcaaagc cccggacagt tccgtgatgt gccgtttggc gaaggctgcg
     6001 tcgatttcgt tggcatcttc aaaacgctgc atgaactgaa ctatcgcggt tctttcctga
     6061 ttgagatgtg gaccgaaaaa gccaaagagc cggtgctgga gattattcag gcgcggcgct
     6121 ggattgaagc gcgtatgcag gaggctggat ttatatgtta gagcaactga aagccgacgt
     6181 gctggcggca aatctggcac ttcccgctca ccatctggtg acgttcacct ggggcaatgt
     6241 cagcgcggta gacgaaacgc ggcaatggat ggtaatcaaa ccttccggcg tcgagtacga
     6301 cgtgatgacc gccgacgata tggtggtggt tgagatagcc agcggtaagg tggtggaagg
     6361 cagcaaaaag ccctcgtccg acacgccaac gcatctggcg ctctaccgtt gctatgccga
     6421 aatcggtggc attgtgcata cccactcgcg ccacgccacc atctggtcac aggccgggct
     6481 ggatctcccc gcctggggca ccacgcacgc cgattacttc tatggagcca tcccctgcac
     6541 gcgcctgatg accgcagaag agatcaacgg cgagtatgaa taccagactg gcgaagtgat
     6601 cattgaaacc ctcgaagaac gcggcaggag tccagcgcaa atcccggcgg ttctggtaca
     6661 ttctcacggc ccgttcgcct ggggaaaaaa tgccgccgat gcggtgcata acgccgtggt
     6721 gctggaagag tgtgcctata tgggcctgtt ctcgcgtcag cttgcgccgc agcttcctgc
     6781 gatgcaaaac gaactgttgg ataagcacta cctgcgtaag catggggcca atgcctatta
     6841 cgggcagtaa tccctcacgc cggggcttca ttgccccggc actacgaatt gatatgttcc
     6901 ttgctgtaac gtcgcttcca cgcagctggc gttaaaccgg tgtgtttgcg aaaaatctgc
     6961 cggaaatagc caacgtcatt aaacccgcac aacgccgcca cttcagtgag cgatcgcgct
     7021 tcgctaatta acagcttttc ggcttcgcgc actcgctggc gatgaattgc ttctgttaac
     7081 gtcagatgaa acacccgccg atagacacgc ccaagataat cggcattgca gtacagctcg
     7141 cgggctaaaa gcgccgtaga aagcgggcga tgatattgcg tgcggatcag tttctgcgct
     7201 ttccaggcaa gcgtggtacc tgcggcatcg gcctgttcat caagacgcgc gtcacgggtg
     7261 agctgctgca aaatcagcag cacaataaac tccagcgccg gagtgcgttg atgcttttcc
     7321 tgttcgctta agaactggcg aaacagggcc accagcgcct gcggatcggc aaccttcgtt
     7381 tgttgcggca cgctaagatg agtgagccag cgcgggtcgc tatccggcgc tttaacctca
     7441 aaatgcagcc agtaaaattt gagatcggcg ggaaaatcct ctaccccaac atgctgtcgg
     7501 ttcggccaga gtaataaact ctctcccgca tcgaccagaa atatccgctc ttcttcctga
     7561 atcgctaatc gacctttttc gacaaagata atttcccatg actccagccg ccttgccgga
     7621 tgcctcccta cgccacggga aataaataat ccaccatttt gcactttaat cggtaacgtc
     7681 atggataatt cgagcattga tatgtaattt cccgcttatt cattaatgat taaacacatt
     7741 accttttgat tattaatcaa aaacattaat ttcacctgat ccatttcaca cttttaaatt
     7801 caggtcggaa tcgtcacctt tttttactct tttctcgcct tgttgatatt cccgttcacg
     7861 gcagaattaa tcaggtcatt gcaataaaac taaaaattgc tccctattca cgaggaatat
     7921 ctatgacctc tacaccgatt actaccgctg atatcgctaa aggtaaaatt gacgatgcgt
     7981 tatctgtacg ggaaaaaata ggctacggcc tgggtgacgc aggcggcacc gtaataactt
     8041 gcctgatcat gaactttctc acctttttct acaccgacgt ttttggctta actccggcgc
     8101 tggtcggcac gctgtttatt gcactgcgcg tgtttgatgc catctccgac ccggtgatgg
     8161 gcgtcattgc cgaccggacg caaagtcgct gggggcgctt tcgtccgtgg cagttgtgga
     8221 ttgccattcc catcggcatt atcggcatcc tgacgttcac cgtgccagat gccagcatgg
     8281 gagtaaaaat cgcctgggcg ttcggcactt acctgctcct ttcagtcggt tataccgcca
     8341 tcaacgtgcc gtactgcgcg ctgatcaaca ccatgaccac ccgccacaat gaagtgatct
     8401 cctgccagtc ctggcgattc gttctctgcg gcgtggcggg atttctggtt tcggtaggct
     8461 taccgtggat ggtagatctc ttcggtcagg gtaatgccgc gcgcggctat caactgggcg
     8521 tcggcgtatt gtgcgccatt gccgtggtga tgttcctgtg ctgtttcttc tgggttcgtg
     8581 aacgggtgcc actctccaca atggggaaat ttaccctgcg cgaacatctt gccgggctgc
     8641 gaaacaacga ccaactactg ctgatgctgg tcatgtcttt cctgctgatt aacgtcttta
     8701 acattcgcgg cggtgggtat atgtacttca ttacctacgt cttacaaggc agtacgggct
     8761 acacgtcgct gttcttcacc atggtcacct tcgcctccat tatcggctcg gtgattgtca
     8821 gcccattaac gcggcgtttc gataccgtca aaatttacta ctacaccaac ctgctcctcg
     8881 ctgcgctggc ggtgttgatg tggttcctgc cctctggccc ggcttatcaa acgctgtggc
     8941 tggcggtgat cctcggtaat ggcgtgattc ttggcttcac attgccactg cacttctcat
     9001 tgatggcctt tgccgatgac tacggcgagt ggaaaacccg cgtacgttct tccggcatga
     9061 acttcgcctt caatctgttt ttcatcaagc tggcctgggc ctccagcgcc gggatcatca
     9121 gcctgctgtt tatttttgtc gcctaccagc caggcgtgga aaaccagacc gccagttcgc
     9181 ttggcgggat cacagcaatg gaaacattgc tgcctgcgct attccacctg ctgctggcga
     9241 tggcgatccg cttttgcaaa ctcaataacc ctatgatgtc acgcattgct agcgacctgc
     9301 gtcagcgtca tgtacagcct taaggagcac aagatgaaca tttcggaagt cgatctgcat
     9361 aaactgacgg tcagcgatcc gttcctcggt cagtaccaac aactggtccg cgatgtggtg
     9421 attccttatc agtgggacgc cttgaacgat cgtatcccag aagcggaacc cagccatgcg
     9481 attgaaaact ttcgcattgc cgcaggactg caagacggtg aattttacgg gatggtgttt
     9541 caggacagcg acgtcgccaa atggctggaa gcggtagcct ggtcgctgtg ccagaagccg
     9601 gatgccgaac tggaaaaaac tgctgacgag gtgattgaac tgatcgcctc cgcccagtgc
     9661 gaagatggct atctcaatac ttactttacg gtaaaagcgc ccgaagaacg ctggagcaat
     9721 ctggcggagt gtcatgaact ttactgcgcc ggtcatctga ttgaagccgg agtcgccttc
     9781 ttccaggcca cgggcaagcg gcgcttgctg gaagtggttt gccgtctggc cgatcatatc
     9841 gacagcgtat ttggtccagg tgaaagtaag ttacacggtt atcctggtca cccggaaatt
     9901 gaactggcac taatgcgcct gtatgaagtg accgaagagc cgcgctacct ggcgctgacg
     9961 aactattttg tcgaacagcg tggtgcgcaa ccgcactatt acgaccagga atatgaaaag
    10021 cgcgggcaga cctcgcactg gcacacctac ggcccggcgt ggatggtgaa agataaagcc
    10081 tacagccagg cgcatttgcc catcgcacag caacaaaccg ccatcggaca tgcagtacgc
    10141 tttgtctacc tgatgactgg cgtcgcacat ctggcgcgtt taagtcacga tgaaagcaag
    10201 cgtcaggatt gcctgcggct gtggaacaat atggcccagc gtcagttata tattaccggc
    10261 ggcattggct cacaaagcag cggcgaagcg tttagcagcg attacgatct gccgaatgac
    10321 acggtttacg ccgaaagttg tgcttccatc ggcctgatga tgttcgcccg acgaatgctg
    10381 gaaatggaag gcgacagtca atatgccgat gtgatggagc gcgcactgta caacactgtg
    10441 ctcggcggca tggcactgga tggcaaacat ttcttctatg tgaatccgct ggaagtacat
    10501 ccaaaatcgc tgaaattcaa ccatatctac gatcacgtta aaccgatccg ccagcgttgg
    10561 tttggctgcg cttgttgtcc gccaaatatc gcccgcgtgc tgacctcgat tggtcattat
    10621 ctctacacac cgcgtgaaga tgcgttgtat atcaacatct acgcaggaaa cagcatggaa
    10681 gtgccggtag aaaatggcac gttgcgcctg cgggttagcg ggaactatcc gtggcaggaa
    10741 caggtgacaa ttgcggttga atcgccccag ccggtgcgtc atacgctggc tttacgtctg
    10801 ccggactggt gcacacagcc gcagatcata ttgaatgggg aagaggtcga gcaggatatt
    10861 cgtaaagggt atttgcacat tacccgcgaa tggcaggagg gcgatacgct gaatctgact
    10921 ttgccgatgc cggtacgccg cgtttacggt aaccctctgg tgcgtcacgt cgccggaaaa
    10981 gtggcgattc agcgcggccc gctggtgtat tgcctggaac aggccgacaa cggcgagtca
    11041 ctgcataacc tgtggctgcc cgccgatgcg ccatttacga catttgaagg taagggattg
    11101 ttcagccata agatcttaat ccaggcaccg ggttaccggt atgaacagag caatccagag
    11161 cagcaaccgc tgtggcatta cgacagcgcg ccagccaaac gccagccgca aattctgacg
    11221 tttatcccgt ggtttagctg ggccaaccgg ggtgaaggcg aaatgcggat ctgggtgaat
    11281 gaggaaaagc attgccatcc gtaggttgga taaggcactc gcgccgcatc caccgtaggg
    11341 tgcacattgc ctgatgcgac gcttaacacg tcttatcagg cctacaagtc ggtgcgagag
    11401 cgtaggccag ccgcaccgac atccaccggt gaattaaaaa cgccagctca cgccagtgac
    11461 aaaggtaaaa ctctcattac gatcaaccat cgggctgtcg gtaatctctt ccggcagctc
    11521 gctgtatcct gcgctcgcca tcaatacgac gtgctctcct atcgggtatt ttgccgtcag
    11581 gctgacatag ggcacccagg catcctgcgc ggaataactt gccagaccgc tacgacggga
    11641 ctcactctct gaaatgccat agtaatagtc actgaaattc tcgtcataat agagaacgcc
    11701 cagcgcaggt gtcagcgaca ggcgacctat ctgcattttg tggaataccg atagctcccc
    11761 cacccagccg ttgctgttat ccagaacgtc cgcagccgca gaggctttca cggttcccca
    11821 acgctcgtgg tgataccagg caacccccgc catagccgta ctatcacgct tatcaagctg
    11881 ttgcatggca taatcgtcat tatcggcagg gtcaaattcc agcggcatcc aggatgcggt
    11941 caggctaagt tcattttttt ca
//
LOCUS       DARNVM010000149        11931 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.149, whole genome shotgun sequence.
ACCESSION   DARNVM010000149 DARNVM010000000
VERSION     DARNVM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.149"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            70..1404
                     /gene="adeQ"
                     /locus_tag="SI621_003724"
                     /gene_synonym="yicO"
     CDS             70..1404
                     /gene="adeQ"
                     /locus_tag="SI621_003724"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HEI4066386.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGIYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1457..1909
                     /locus_tag="SI621_003725"
     CDS             1457..1909
                     /locus_tag="SI621_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HEI4066387.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2025..2348)
                     /locus_tag="SI621_003726"
     CDS             complement(2025..2348)
                     /locus_tag="SI621_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEI4066388.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2332..2685)
                     /locus_tag="SI621_003727"
     CDS             complement(2332..2685)
                     /locus_tag="SI621_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4066389.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2918..4102
                     /gene="nepI"
                     /locus_tag="SI621_003728"
     CDS             2918..4102
                     /gene="nepI"
                     /locus_tag="SI621_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HEI4066390.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMLAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4304..>11931
                     /locus_tag="SI621_003729"
     CDS             4304..>11931
                     /locus_tag="SI621_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEI4066391.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINPAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDNRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKVVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLAGSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAETNGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSSVVEVSGTDKNETGNWSEESDGVYTSTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTAYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKINQSSDSKT
                     VNFIADVSTAQVAELVVTQDGSVADGSTANMLRVRVTDVFGNVLAGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGTSAVTASINNSSQSRNVTFIADVSTAQIADLVVTR
                     DNSVADGAMANTLRVRVTDAFGNALAGQTVSVLADNGATVTPTVITGQDGTVEISVTS
                     QTAGTSAVTATINSSSQSRDVTFVADVRTAKIADLVVIKDDSVADGAMANMLRARVTD
                     AFGNALAGQTVSVLAGNGATTAPTVTTQPDGTVEISVTSQTAGTSAVTASINSSSQSR
                     NVTFIADVSTAQIASLEVMQDNAVADGATANTLRVRVTDAFGNALGGQTVSVMADNGA
                     TVSPTVSTGPDGTVEISVTSQTAGTSAVTASINSSTLSRNVTFVADVRTAQIADLVVI
                     KDDSVADGAMANTLRVRVTDAFGNALAGQTVSVMAGNGATVAPTVITEPDGTVEISVT
                     SQTAGISAVTASINSSSQSRDVTFIADVRTAKIAELEVIRDNAVADGSTANTLQVKVT
                     DANDNTLAGQAVSVLAGNSATVASTVTTKPDGTVEISVTSQTAGTSTVTASINSSSLS
                     RNVTFVADVSTAKIADLVVIQDNSVADGAMANTLRMRVTDAFGNTLGGQTVSVTADNS
                     AMVASTVITGPDGTVEISVTSQTAGISIVTASINNSSLSRDVTFVADVRTAQIADLVV
                     IKDGSEADGLTANTLQVRVTDAFGNALAGQTVSVLADNGATVAPTVTTQPDGTVEISV
                     TSQTAGVSAVTATINSSSQSQNVTFIADVRTAKIADLVVIKDGSEADGSTANTLRARV
                     TDAFGNALAGQAVSVMAGNSATVTPTVTTQSDGTVEFSVTSQTAGTSTVTASINSSSL
                     SRDVTFIADVRTAQIAVLEVTQDYAVADGSTANTLRARVTDAFGNALAGQTVSVLGGN
                     GATVSPTVITGPDGTVEISVTSQTAGASTVTASINSSSLSRNVTFVADVRTAQIAVLE
                     VTQDYAVADGSTANTLRARVTDAFGNALAGQTVSVTAGNGATVSPTVITGPDGTVEIS
                     VTSQTAGVSAVTATINNSTASQNVMFIADVRTAKIADLVVIKDDSVADGAMANMLRAR
                     VTDAFGNALAGQTVSVMAGNGATTAPTVTTQPDGTVEISATSQTAGISTVTATINNSS
                     LSRNVMFVADVRTAQIADLVVIKDGSVADGSTANMLRVRVTDAFGNALGGQTVSVLAD
                     NGVTTAPTVITEPDGTVEISVTSQTAGVSAVTATINSSSQSQNVTFIADVSTAKIADL
                     VVIKDGSEADGSTANTLRVRVTDAFGNTLAGQTVSVTAGNGATTAPTVITEPDGTVEI
                     SVTSQTAGVSAVTASINSSSQSRNVTFVADVRTAQIADLVVIKDGSEADGATANTLRA
                     RVTDA"
BASE COUNT         2732 a         2933 c         3540 g         2726 t
ORIGIN      
        1 atcactgaac cgttgttgtc atttgtttta caaaagcaag ggattaaatc tcctccaatg
       61 gacaaaaaaa tgaataatga caataccgat tacgtgagta atgaatcagg gacgctttcg
      121 cgattattta aactacctca gcatgggacc accgtccgca cagaattgat tgcggggatg
      181 accacttttt taaccatggt gtacatcgtt tttgtgaacc cgcaaatcct cggcgcggca
      241 caaatggacc cgaaagtggt gtttgttacc acctgtttga ttgccggtat cggcagtatt
      301 gcgatgggga tatttgctaa cttacccgtg gcgctggctc cggcaatggg gctgaacgcc
      361 ttctttgcgt tcgtggtcgt gggggcgatg ggtatctctt ggcagaccgg gatgggcgcg
      421 atattctggg gcgcaattgg gctatttttg ctcacgctgt ttcgtatccg ttactggatg
      481 atctccaaca ttccattaag tttacgtatt ggcatcacca gcggaatcgg attatttatt
      541 gccttaatgg gattaaaaaa cactggcgtt attgtcgcca ataaagacac cctggtgatg
      601 attggcgatt taagttctca cggcgtgttg ttaggtattt tagggttttt tattataacc
      661 gtgttgtcat cacgtcattt tcatgccgcg gtgctggttt ctattgtggt gacgtcttgc
      721 tgtggattat ttttcggtga tgttcatttt agcggcatct attccattcc gcctgatatt
      781 agcggcgtca ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc
      841 attttctcct ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact
      901 gataaagctg gcttaataga tagtaacggt aaattcccca atatgaataa ggcgctatat
      961 gttgatagcg tcagttcggt ggcgggcgcg tttatcggca cctcgtctgt taccgcctat
     1021 attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg ggctgactgc ggttgtggtc
     1081 ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg tggcgatggt tcctccttac
     1141 gcaaccgccg gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt
     1201 aactgggatg attttaccga atcggtgccc gcatttatta ccacggtgat gatgcccttt
     1261 actttctcga ttaccgaagg gattgcactt ggctttatgt cgtactgcat catgaaagtg
     1321 tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca
     1381 ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga
     1441 ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt
     1501 tggttttcgg gtgctgacat ccggggccag aaaagcgatt cgccgtctca gcgatcggct
     1561 gaatatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga
     1621 cgaattttta cgttatttgc atcgtccgga tgagtcgcac ctgcaaaacg ccgcgcaggt
     1681 gttgctcatc tggcaaattg tcattgtcga tggtagcgaa cagaatctgc tgcaatggca
     1741 tcggatttta caaaaagccc gccttgccgc gccgattact gatgctcagg tcaggctggc
     1801 gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg
     1861 ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt
     1921 cttatcagcc tacagaccgc gcctgacctg tagggtggat aaggcgttca cgccgcatcc
     1981 ggtattggaa ctgacagtag gtgaatgcat tatctacgtt ctatttacag cagcacctcc
     2041 agcccgtgtt tttgcacaat gctgagcagt tttaatgaaa tgccattggg gcgtttagcg
     2101 ccactttccc atttttgtac agttgaaacg ctggtattga gatagtgagc gaagacgggc
     2161 tgactcactt ttaatttttc ccgcagtgct tttatctcaa caggttgtaa gtcttgcacg
     2221 ctggtcaggc aggctttgtc aaaagtacgc atcgtttcgg cagaaatagc atcgacgctg
     2281 gacaatccag ccgctgcgct gtgaatagcc tcaaatgcag gacttttaaa tttagttttt
     2341 gcagtcatag cagatctctg tcagttcttt agtgttaatc attgctgtta gttgggcttt
     2401 cgtcaggaag gcataatgct ttgcaatttc acgaaaccca gccagttcct gactattgat
     2461 gttcgacata tcctgttttg catataaaaa cgtgtaaaac caattgcttc ctcctttcgc
     2521 cagaataatt gcacgctcac gattttggtg aaggcgcttt ttaaaaacgc caccgccaag
     2581 tgcatcagct ttaccttgtt ccgtttctaa aatggcccgg cacaactctt catctgttat
     2641 tgcatgagac ctggcttccc ttgcaaacca tttggtttta aacacgcgca tatcgttgag
     2701 gtcctgttat taaagtatat caccaagtgc aatactttat cccttgttcc caacacaatc
     2761 gcgaaaatac atatcacctt gccctatgat ttttatttct tcgagcagcc tcgaactttc
     2821 ccacaacacc gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa tgttaaagtg
     2881 gtcggctttt cccctgaaac atgccacggg taacaccatg agtgaattta ttgccgaaaa
     2941 ccgcggcgcg gatgccatca cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg
     3001 cgtcgcctgt ctgattatcg ttgagttttt gcctgtcagt ttgttgacgc caatggccca
     3061 ggatttaggc atttcggaag gggttgccgg gcaatcggtg accgtgaccg cctttgtggc
     3121 aatgtttgcc agtttgttta ttacccagac aattcaggcc actgaccgcc gctacgttgt
     3181 tattttgttt gccgttttgc tgacgctctc ctgcttgctg gtttcctttg ctaactcatt
     3241 cagtttgctt ttaatcggtc gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat
     3301 gtcggcgtcg ctgaccatgc gtctggtacc gccgcgtacg gtgccgaagg cgctgtcggt
     3361 gatcttcggc gcggtttcta ttgcgctggt gattgctgcg ccgttgggca gttttttagg
     3421 cgagcttatc ggttggcgca atgtctttaa tgcggcggcg gcgatgggcg tgttgtgtat
     3481 tttctggatt atcaaatcat tgccttcgct gccaggcgaa ccctcgcatc agaaacaaaa
     3541 tactttccgc ttattacaac gtccgggtgt gatggcaggg atgctcgcca tcttcatgtc
     3601 tttcgccggg cagtttgctt tcttcacgta tattcgcccg gtgtatatga acctggcggg
     3661 attcggcgtg gatggcttaa cgctggtgct gttgagtttt ggtatcgcca gctttattgg
     3721 tacgtcgctt tcgtcgttca ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc
     3781 gttaatactg gctgtgagtg cgttggtgct gacgttgtgg ggaagcgata aaatcgttgc
     3841 taccggcgtg gcgattatct gggggctaac ttttgcattg gttcctgtcg gctggtcaac
     3901 gtggatcacc cgctcactgg ccgatcaggc agaaaaagcc gggtctattc aggtggcggt
     3961 tattcagctt gctaatacct gtggcgcggc aatcggcggt tatgcgctgg ataatattgg
     4021 tctgacttcg ccgttgatgt tgtccggcac attgatgttg ctgacggcgt tattggtcac
     4081 cgcaaaggtg aaaatgaagt aacgtattta caggaggggt tactgctctg gcgggaaagc
     4141 tctcctgttc ttcccctgat aaccattgat caggcattgt tcttatcttg ttgttatttc
     4201 cattattccg cctgtattca tcatttgcta atcatgaatc atcattttct ctcatgttaa
     4261 agaaaggtca tttaatctga acgcatagcg acagggatga caaatggcag ggaaagcgca
     4321 tgggaatggc gatcgtcggg gcgataacac catatgtgga ttgggggatc gtttacgtcg
     4381 tctgaccgcc ggtatttgcc tgataacaca aactattttc cctgttatgg ctgctgcccc
     4441 tacgcatatt aatcctgcgc actcggatac tgccgcgtca ctgatcctgc cgaacgttaa
     4501 aactattcca tataccctgg gtgcgttgga atcgccacca acggtcgcgg cacgctttgg
     4561 tatcaccgtt gatgaattac gtcgcctgaa tcagttccgt acctttgcgc ggggctttga
     4621 caatgtgcgt cagggcgatg aaatagatgt gcccctcatt aacagtaaca gccctgaagc
     4681 gcgcaacctg aaagcaatgc agatggagcg cgacggcaaa gatccgcaaa tgcaggtcgc
     4741 ggaaatggcg cagcaaagcg gaacactttt agctcgcgat atggacagcg aacaggcggc
     4801 gagtatggcg cgtggctggg tggcttcttc tgcatctgcg caggcaacgg actggctcag
     4861 ccgttgggga actgcccggg tatctcttgg tgtggatgaa gatttcagcc ttaaaagctc
     4921 gtcatttgaa tttctccatc cctggtacga aacgccggat aacctggtct tcagccagca
     4981 cacacttcac cgtacggata atcgcactca aaccaaccac ggtatcggct ggcgttattt
     5041 cacatcgagc tggatgtctg gcgtcaatat gtttatcgat catgacctga cccgctatca
     5101 cacccgaacc gggatgggcg ttgaatactg gcgcgattac ctgaaactga gcggcaacgg
     5161 ttatctgcgc ctcagtaact ggcgtagtgc accggaactc gacaatgact atgaagcacg
     5221 tccggcgaac ggttgggatc ttcgcgccga aggctggtta ccggcctggc cgcagctggg
     5281 cggcaaggtg gtctatgaac aatattatgg cgatgaagtg gcgctgtttg gtaaagacga
     5341 gcgtcaaaat gatcctcacg cgattaccgc aggtttgagt tatacccctg ttcctctgat
     5401 ctcctttagt gccgagcaac ggcagggcaa acagggagaa aacgacaccc gaattggtat
     5461 ggagttgacg ttgcaacccg gccattcact gcaaaaacag ctcgatccgg ctgaagttgc
     5521 agcacgacgt agccttgtgg gcagtcgcta tgacctggtc gatcgtaata acaatattgt
     5581 cctcgaatat cgcaagaaag agctggtgcg gctgacgctg accgatccgt tgaaaggcaa
     5641 gccgggtgag gttaaatcgc tggtttcttc cttacaaacc aaatatgcgc tgaaaggtta
     5701 tgacattgaa gccgcctccc tgcaatcagc gggcggaaaa gtagctgttt ccggcaagga
     5761 tattcaggtc actattcctc cctaccgatt cacggcaatg cctgaaaccg acaacactta
     5821 cccgatagcg gttacagcgg aagattcgaa aggaaatttc tcccggcgtg aagaaagtat
     5881 ggtggtcgtg gaaaaaccca cattaagcct cgccggttca acgctttctg tcgatctgca
     5941 aatcctgctt gctgatggaa aatcaacctc aacgctgacc tataccgcgc gagattccag
     6001 cggcaagcca atcccaggta tgacgttaaa aacacaggtg aaaggtttgc aggatttcgc
     6061 cttaagcgaa tggaaagaca acggtaacgg aacctatacc caaatagtta ccgccgggaa
     6121 gacgtctggc gcgttatcgc tgatgccgca atttaacggc gatgatatcg ctaagacgcc
     6181 agcgctgatt gctatcgtgg cgaatacagc atcccgcgct gactccacga ttgagacaga
     6241 ccaggataat tacgtcgcgg gtaagccgat agtcgttaaa gtgaccctga gagatgataa
     6301 cggcaacggg gttaccggac gaaaagaact tctcaaacaa accgttaaag ttgacaacac
     6361 gaaggctgat gccgttagtg cctggacgga agaaagtgag ggaatttaca aagcaagcta
     6421 tacggcccat ctcattggcg acaaattaac ggcccagctg acgatgcccg gctggcaaac
     6481 caaacattcg gatgcgttca gtattgcggg tgataaagat accgccaaaa tcgctgcgat
     6541 gcagatcacc gcgaataacg cagttgccag acgcgatcac aataccgtcg ctgtgacggt
     6601 cagggatgtg catcaaaatt tactgcaggg acaaaatgtt accttcacgg tcgtgaatgg
     6661 tgcggcggtt tttgccgatc caaatggcgg tattgtgacg acagataaag acgggattgc
     6721 cagcgttaac cttgccagtg accaggcggt aaacagtctt atcaaggccg agaccaatgg
     6781 cagtagtcag tctgtggaag tgagctttat tactggcgat atctcgcagc tgacctccac
     6841 catcaagaca gacgatgtgt catacaccgc gggtggcaag ataaaagtgt cagttaccct
     6901 tatggatgag cagaaaaacc tggtgaaagg gatggcttcg ctgctggcgg gtagcagtgt
     6961 ggtcgaggtg agcggtacag ataaaaatga aacgggtaac tggagcgaag agtctgatgg
     7021 cgtttacacc agcacccgaa ccgcgaagat tgctggcgat cgccattacg ccacgctgaa
     7081 actatcgaca tggagtagcg cgcagcaatc tgatgcgtat gccatacgcg aaagcggagc
     7141 tgtgttggct tattcctcca tcgttaccga caaaacggcc tacaccgcag gcggtgcgat
     7201 aaaagttacg gtaacactca aggatagcta tgaaaacctg gtcggggggc aacgagacgc
     7261 tatcaaccag gctatccagc tgccaaatac aaaagcggaa agcatcgcct ggaatgaaga
     7321 tcaaaaagga atttataccg caacgtatac cgccttactc cctggcaccg gtttaaaagc
     7381 acagctacag atgtccggct gggcgagcgc tctgacttca aatgattaca gtattagtgg
     7441 cgatgccgcc agcgcacaaa ttgtcgcgat gcaagtgaca acgggtaacc cggatgtgct
     7501 ggcgaatggc agcgatcgac atacggtgaa tgtccgggtg gaagatcagt ttggcaatgt
     7561 gctgccggag cagaccgtca cctttacggt gacgaagggg gcggctgtat ttgccaatgc
     7621 agggcagagc gcagatatca ggacggatgc gcatggcatg gcagaggttg atctgagcag
     7681 taccgtagcg gatgccagca ctgttgaggc taagatcaat caaagcagcg atagtaaaac
     7741 ggtgaacttt attgctgatg ttagcacggc gcaggtcgct gagctggtgg tgacgcagga
     7801 cggctcagtg gctgatggtt cgacggcgaa catgctgcgg gtgagggtga ccgatgtgtt
     7861 cgggaatgta cttgccggcc agacggtcag cgtgttggcg gacaacggcg caacggtcgc
     7921 tccgacggtg attacagagc cggacggcac ggtggagatc tcagtcacca gccagaccgc
     7981 cggaaccagt gcggtcaccg ccagcatcaa caacagcagc cagagccgga acgtgacgtt
     8041 tattgcggat gtcagcacgg cgcagatcgc ggatctggtg gtcacccggg ataactcggt
     8101 ggctgacggt gcaatggcga acacgctgcg ggtgagggtc acggatgcgt tcggtaatgc
     8161 gcttgccggg cagacggtca gcgtgttggc ggataacggc gcaacggtca ctccgactgt
     8221 catcacaggg caggacggga cggtggagat cagtgtcacc agccagacgg cgggcaccag
     8281 tgccgtcact gcaaccatca acagcagcag ccagagccgg gatgtgacgt ttgttgccga
     8341 tgtcaggacg gcgaagatcg ctgatctggt ggtcattaag gacgactcag tggctgacgg
     8401 tgcaatggcg aatatgctgc gggcgagggt caccgatgcg ttcggtaatg cgcttgccgg
     8461 gcagacggtc agcgtgttgg caggcaacgg tgccacgact gctccgacgg tcaccacgca
     8521 gccggacggc acggtggaga tctctgtcac cagccagacg gcgggcacca gtgcggtcac
     8581 tgccagcatt aacagcagca gccagagccg gaacgtgacg tttatcgccg atgtcagcac
     8641 ggcgcagatc gcgagtctgg aggtgatgca ggataacgcc gtggcagacg gtgcgacggc
     8701 gaacacgctg cgggtgaggg tgactgatgc attcggtaat gcgcttggcg gacagacggt
     8761 cagcgtgatg gcagacaacg gtgcaacggt gtctccgacg gtcagcacag ggccggacgg
     8821 cacggtggaa atcagtgtca ccagccagac ggcaggcacc agtgcggtca ctgccagcat
     8881 taacagcagc accctgagcc ggaacgtcac gtttgtcgcg gatgtcagga cggcgcagat
     8941 cgcggacctg gtggtcatta aggacgactc agtggcagac ggtgcaatgg cgaatacgtt
     9001 gcgggtgagg gtgaccgatg cgttcggtaa tgcgcttgcc ggacagacgg tcagcgtgat
     9061 ggcaggcaac ggcgcaacgg ttgccccgac ggtgatcacc gagccggacg gcacggtgga
     9121 aatcagtgtc accagtcaga cggcgggaat cagtgcagtc acagccagca tcaacagcag
     9181 cagtcagagc cgggacgtga catttatcgc cgatgtcagg acggcgaaga tcgctgaact
     9241 ggaggtcatc cgggataacg cggtggcaga cggttcgacg gcgaatacgc tgcaggtgaa
     9301 ggtcaccgat gctaacgata atacgcttgc cggacaggcg gtcagcgtgt tggcaggcaa
     9361 cagcgcaacg gttgcttcga cggtgaccac aaagccggat ggcacggtgg agatcagtgt
     9421 caccagtcag acggcaggca ccagtacagt cactgccagc atcaacagca gcagcctgag
     9481 ccggaacgtg acgtttgtcg ccgatgtcag cacggcaaaa atagcggatc tggtcgttat
     9541 ccaggataac tcagtggctg acggcgcaat ggcgaacacg ctgcggatga gggtcacgga
     9601 tgcattcggt aatacgcttg gcggacagac ggtcagcgtg acggcagaca acagtgcaat
     9661 ggtcgcttcg actgtcatca cagggccgga cggtacggtg gagatcagtg tcaccagcca
     9721 gacggcgggg atcagtattg tcactgcatc catcaacaac agcagcctga gccgggacgt
     9781 gacgtttgtt gccgatgtca ggacggcgca gatcgctgat ctggtggtca ttaaggatgg
     9841 ttcagaggcg gacggtttga cggcgaacac gctgcaggtg agggtcaccg atgcgttcgg
     9901 taatgcgctt gccgggcaga cggtcagcgt gttggcggac aacggcgcaa cggtcgcccc
     9961 gacggtcacc acgcagccgg acggtacggt ggagatctct gttaccagcc agacggcggg
    10021 agtcagtgca gtcactgcaa ccatcaacag cagtagtcag agtcagaacg tgacatttat
    10081 cgccgatgtc aggacggcga agatcgcgga tctggtggtc attaaggatg gttcagaggc
    10141 ggacggttcg acggcgaaca cgctgcgggc gagggtgacc gatgcgttcg gcaatgcact
    10201 tgccgggcag gcggtcagcg tgatggcagg caacagcgca acggtcactc cgacggtcac
    10261 cacgcagtcg gacggtacgg tggagttcag tgtcaccagc cagacggcag gaaccagtac
    10321 agtcaccgcc agtatcaaca gcagcagcct gagccgggac gtgacgttta ttgcggatgt
    10381 caggacggcg cagatcgcgg tgctggaagt gacgcaggat tacgccgtgg cagatggttc
    10441 gacggcgaac acgctgcggg cgagggtgac cgatgcgttc ggtaatgcgc ttgccgggca
    10501 gacggtcagc gtgttgggag gcaacggcgc aacggtgtct ccgacggtca tcacagggcc
    10561 ggacggcacg gtggagatca gtgtcaccag tcagacggcg ggagccagta ctgtcactgc
    10621 cagcatcaac agcagcagcc tgagccggaa cgtgacgttt gtcgcggatg tcaggacggc
    10681 gcagatcgcg gtgctggaag tgacgcagga ttacgccgtg gcagatggtt cgacggcgaa
    10741 cacgctgcgg gcgagggtga ccgatgcgtt cgggaatgca cttgccgggc agacggtcag
    10801 cgtgacggca ggcaacggcg caacggtgtc tccgacggtc atcacagggc cggacggcac
    10861 ggtggaaatc agtgtcacca gccagacggc gggagtcagt gcagtcactg caaccatcaa
    10921 caacagcacc gcgagccaga acgtgatgtt tatcgccgat gtcaggacgg cgaagatcgc
    10981 ggatctggtg gtcattaagg acgactcagt ggcagacggt gcaatggcga atatgctgcg
    11041 ggcgagggtc accgatgcgt tcggtaatgc gcttgccgga cagacggtca gcgtgatggc
    11101 aggcaacggt gccacgactg ctccgacggt caccacgcag ccggacggca cggtggagat
    11161 ctctgccacc agccagacgg cggggatcag tacagtcact gcaaccatca acaacagcag
    11221 cctgagccgg aacgtgatgt ttgtcgcgga tgtcaggacg gcgcagatcg ctgatctggt
    11281 ggtcattaag gacggctcag tggcagacgg ttcgacggcg aacatgctgc gggtgagggt
    11341 gacggatgcg ttcggtaatg cgcttggcgg ccagacggtc agcgtgttgg cggacaacgg
    11401 cgtaacgact gccccaacgg tgatcaccga gccggacggc acggtggaaa tcagtgtcac
    11461 cagccagacg gcgggagtca gtgcagtcac tgcaaccatc aacagcagta gtcagagtca
    11521 gaacgtgaca tttatcgccg atgtcagcac ggcgaagatc gctgatctgg tggtcattaa
    11581 ggatggttca gaggcggacg gttcgacggc gaacacgctg cgggtgaggg tgaccgatgc
    11641 gttcgggaac acgctagccg ggcagacggt cagcgtgacg gcaggcaacg gcgcaacgac
    11701 tgccccgacg gtcatcacag agccggacgg tacggtggag atctcagtca ccagccagac
    11761 ggcgggagtc agtgcagtca ctgccagcat caacagcagc agccagagcc ggaacgtgac
    11821 gtttgtcgcc gatgtcagga cggcgcagat cgcggatctg gtggttatta aggacggttc
    11881 agaggcggac ggtgcgacgg cgaacacgct gcgggcgagg gtgaccgatg c
//
LOCUS       DARNVM010000150        11747 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.150, whole genome shotgun sequence.
ACCESSION   DARNVM010000150 DARNVM010000000
VERSION     DARNVM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.150"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..213
                     /locus_tag="SI621_003730"
     CDS             <1..213
                     /locus_tag="SI621_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HEI4066392.1"
                     /translation="AVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPLQSVPVQYRGLY
                     QIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(210..443)
                     /locus_tag="SI621_003731"
     CDS             complement(210..443)
                     /locus_tag="SI621_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066393.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHSMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPYGC"
     gene            442..849
                     /locus_tag="SI621_003732"
     CDS             442..849
                     /locus_tag="SI621_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HEI4066394.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            846..1400
                     /locus_tag="SI621_003733"
     CDS             846..1400
                     /locus_tag="SI621_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066395.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSGRDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNVAGAESA
                     LNRAGTLAANGVRHYMTITGFTPLADSTVEARARRGRKGATLELARRAAGESPGTELA
                     NPLIDTGQYRRAITHVVRDKDADS"
     gene            1387..1776
                     /locus_tag="SI621_003734"
     CDS             1387..1776
                     /locus_tag="SI621_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HEI4066396.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSNSEVKKQF
                     AGVVTVDRSLENHRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            1751..2314
                     /locus_tag="SI621_003735"
     CDS             1751..2314
                     /locus_tag="SI621_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066397.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPAETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            2318..3463
                     /locus_tag="SI621_003736"
     CDS             2318..3463
                     /locus_tag="SI621_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HEI4066398.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAALVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLVIADDEDIPDADWLKVAAAIESSSVSRILSITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGNLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            3474..3914
                     /locus_tag="SI621_003737"
     CDS             3474..3914
                     /locus_tag="SI621_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HEI4066399.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            3918..4370
                     /locus_tag="SI621_003738"
     CDS             3918..4370
                     /locus_tag="SI621_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066400.1"
                     /translation="MEFEIKGVKYRVAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIETVKFEQLKPVFETMLPRIAEELSSLTEDDTEAIIHPCLAVVSRRHMDGWV
                     PVFTKGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            4548..6536
                     /locus_tag="SI621_003739"
     CDS             4548..6536
                     /locus_tag="SI621_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HEI4066401.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GTALSVVAFTAKIASGLDNLYWASQRTGATVHGIQSIGYAVSQVGGSADAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMASIFTGVGQKLSGMPYYRANQYAQMLG
                     IDENTLMAMRRGVGGFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSS
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKGSVNELVKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQYAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            6536..7123
                     /locus_tag="SI621_003740"
     CDS             6536..7123
                     /locus_tag="SI621_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066402.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSQLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            7123..7425
                     /locus_tag="SI621_003741"
     CDS             7123..7425
                     /locus_tag="SI621_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066403.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLVVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            7428..8492
                     /locus_tag="SI621_003742"
     CDS             7428..8492
                     /locus_tag="SI621_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066404.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSASDVGTVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGVTEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     TNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTSQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGASDIPSQLAMQRGA"
     gene            8495..8956
                     /locus_tag="SI621_003743"
     CDS             8495..8956
                     /locus_tag="SI621_003743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066405.1"
                     /translation="MKKQVIVAITMLISTTSFAEPGKFEFKAADLMEQQKFTEWQHYA
                     SVKPFFSMNGRRFTMTLDDFADIIRNTFQQCNDLDAYTNRKGTRDSCQEYIYNGMKEW
                     IKLSKDPSVSPQAWKIGARYAFNTNNPVPGSNVIDFNGWAGGIRVAKSKGF"
     gene            9130..9900
                     /locus_tag="SI621_003744"
     CDS             9130..9900
                     /locus_tag="SI621_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066406.1"
                     /translation="MARPKLKAEERFDQLINVINSGEPLDMWTFREVVSEYGSNHTPM
                     AEALVALAYIAKGEVLFGIESLEVILPHADVNFARIFCKLLERFSLLEKLDYYIYSLA
                     DKFPTKWFTYKAGGVAYLVGKLSKCVEYLGRHCKMLSEEEHRDDAETFLQEVINDMDE
                     AYKNSGCSSEQYRQVALAVHRVMAEFPSTEYRADINGASGGTYLVEVVKASPERVVAM
                     NMRLADEICSIDLLDDCNLIARFSVERNGLKECKYAYN"
     gene            9887..10282
                     /locus_tag="SI621_003745"
     CDS             9887..10282
                     /locus_tag="SI621_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066407.1"
                     /translation="MPITSSEFLSSAERCFAEDCEIGYRNAISRAYYALYHEIKDNLT
                     SLPAYTRDHHASLISYLKNKGENKLEPYDPRSLKSMAYKLEQQRLARNEADYDLSSCG
                     INKAMAQQSILEVKTIFSQWEQMKTDVAV"
     gene            10348..11100
                     /locus_tag="SI621_003746"
     CDS             10348..11100
                     /locus_tag="SI621_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEI4066408.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIVQSFDPDAVT
                     AVVQPAIRSVEKDNDGNRITKNYPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            11100..11453
                     /locus_tag="SI621_003747"
     CDS             11100..11453
                     /locus_tag="SI621_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066409.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWSQSVLGRQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            11453..>11747
                     /locus_tag="SI621_003748"
     CDS             11453..>11747
                     /locus_tag="SI621_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066410.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALTALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRK"
BASE COUNT         2943 a         2689 c         3295 g         2820 t
ORIGIN      
        1 gcggttaaat ggctgaaagg ccgtggtgtg gggaataagg atcgcatggt tgcgtacacc
       61 aacgataaaa aatacgtccg ctacccgctg gttccgcttc agagcgtgcc ggtgcagtat
      121 cgcggtctgt atcagatcgt cacttactac ggcaagctgg gtgcagtcga gccagtgtac
      181 aaagaaacca tttcgtacgt tgatggcatt taacagccat atggccccct ggcggggcca
      241 ttaaggatga cccgatggca aaaaatgatg cagtaataca cgtacatacc ccgtttgtgc
      301 tcacgcttcc cgacggttct cggcgcgagt ttgttaaagg ccgtcatgct gtggaggaag
      361 acgttgccac gcactggttc actcgtgcgc acgcggaagt atccgttggc aaagccacag
      421 acgcgcgtaa cgaggtaaaa aatgccaaag aatcaaagtc tgccagcggt aagtgatttt
      481 cgccgcgact tcccgcagtt tgctgaccct gccaaatatc ccgaagcgca aatccagttt
      541 cgtctgaatc tggccgatga actgctgagc gaaaacgtca ccggcaaaaa gttgtttccg
      601 tactttgccg ggttgttcgt tgcgcactac atgacgctct gggcggcaga cagcagagcg
      661 atgctggctg gtggtccggg cggttcaacc aatggtgttc agtcctcaaa gtccgtggat
      721 aaggtaagcg tcagttatga caccagcgcg acgctgaatc ctgatgcagg tttctggaat
      781 aacacccgat atggcgctga attttatcag ttgatcacga tgttcggtgc aggtggtcgc
      841 cagctatgag tttcaaaagc ggtgtaacaa cgagggtgga taacgctaag gccattctgg
      901 atgcgctcag gtcgttaacc aaaaaagatg tgctggtcgg catcccttcg gaagacagcg
      961 ggcgggatga tgttccgttt ggtaatgcgg gcatcggtta cctcaacgaa tacggctcac
     1021 cagagcagaa catcccgcca cgacctcacc tggtccccgg cgttaaatcg gcagaagagc
     1081 agacggtgcc gcagctcaaa gccgcggcgc aggctgctct tgatggtaat gttgcgggag
     1141 cagaaagcgc actcaaccgt gccggaacgc tggccgctaa tggcgtcagg cattacatga
     1201 ccattaccgg atttacgccg cttgctgaca gtactgttga agcccgggct cgtcgggggc
     1261 gcaagggggc gacactggaa cttgcccggc gtgctgctgg cgaatctccg ggaaccgaac
     1321 tggcgaaccc attaattgac accgggcaat atcgcagagc tattacccat gttgtgaggg
     1381 ataaagatgc cgactcttga tgtaacagat gtgctttttg accccgattt ttgcgacttc
     1441 aatttgtggg taacacgccg tgtgcaaacg gtggatgagg acgggatcgg cagcaacagc
     1501 gaagttaaaa agcagtttgc cggagtcgta actgttgatc gctctctgga aaaccatcgt
     1561 atgcaggccg ggcaggtaat cagtggtgca attctgattg tgacgactga gcgactgacg
     1621 cagggacaga ctggccgtga tgccgatatc gtgacgtatc agggccgtga ttatcgtgtg
     1681 actttcgtcg acccgtatac agcgtatggt gccggattcg ttcaggcgca ttgtgagttg
     1741 ctgccgtttg atgggggaat tccggttgag caataacacc agcacagagc gcggatggct
     1801 gataccaacc agtggcgatc cggattatga cgaagcgctc gacaggctgt taagccagtg
     1861 gatgcgtaac gtttccggtc tgtctgccgg gatggttcgt ccgcgctggc agaaagagca
     1921 gccgccactg ctaccggctg aaacgaactg gtgtgcgttt ggggttatcg gatggtcagg
     1981 tgatgacagt ccggcattca ccagacagac cgatgatggc tctcagctct ggcggcatga
     2041 aacgattgag tgtatggctt cattttatgg accggcgggg atggtgtatg cgtcccggtt
     2101 tcgtgacggt atatctgtac cgcagaacaa tgcagcactg aatgcgctgg ggctgtctct
     2161 tggcgattac acaggtctga cacccttccc tgaacttatt aatcagcaat gggtccgccg
     2221 ctacgatatg acggtgcgcc tgcgccggaa ggttgtgcgc gagtacggta ttaaatcgct
     2281 ggtggaagca ccagtcatct ttttcggaga ttaagctatg gcacagggct tgcctgtatc
     2341 aaacgttgtt aatgttgatg tgatcatgtc gccgcgtgca gcatcagggc gaaattttgg
     2401 tgcattactc attctcggcc cgtccacaat cattccggta agtgagcgca ttcgccgtta
     2461 ttctgctgcg gaagatattg gaaaagattt tggcgtggaa tcaccagaat ataaggctgc
     2521 gctggtgttt ttctcacaat caccgaaacc tcaggaggtt tttgttggtc gttgggtgaa
     2581 aacgaagggt gacagcgaac aggccacgcc tgagacgctg gagcaggctg tgaatgccat
     2641 gctcgattat acttcatggt atgggctggt gattgcagac gatgaagata ttccggatgc
     2701 agactggctg aaagtggctg cggcgatcga atcctcttct gtaagccgta ttctgtcgat
     2761 tacgacaagc gatgagaaat gcctgcagac tgcatccagc gatgatttgg catcaaaact
     2821 gaaaaccgcc ggatattcac gcagttttat tcagtattca tcgggtaata aatacgctgc
     2881 gttatctgca tttggccggg cattcacggt taatttcaat ggcagtaata ccgcgattac
     2941 gctcaagttt aagcaggagc cgggtgtcgg gtatgaaaca ctgacagtca gccaggcatc
     3001 ggcacttgat gcaaaaaact gcaatgtgtt cgtgtactac cagaatgata cagctatcct
     3061 ccagcaggga gtgatggcta acggcgattt ctttgatgaa cgccacggcc tggactggtt
     3121 acagaattat gtgcagacca acctctataa cctgctttat accagcacca cgaaagttcc
     3181 ccagactgaa gccggtatta cccgactgtt atcaaatgta gaaaaatcac tggatcaggc
     3241 tgttcagaat ggactgattg ctccgggcgt atggaacggt ggcaaccttg ggcagttgtc
     3301 atcaggtgac acgctgccca aaggttatta cgtatacgcc cagccgctgg atgaacaggc
     3361 acaatcagaa cgtgaagccc gtaaggctcc ggtgattcag gctgcaataa aacttgcagg
     3421 cgcggttcat tacgctgacg tacagattaa cgttgttcgc taaggggaag tgaatgtcta
     3481 cctattcttt tatggatgtc actgcgacgc tgaccgggcc gaccggttcg attgacctcg
     3541 ggtacggttc ggcaagttct gaggagggga ttgtggttgc gatgggcggt cctaaaaaca
     3601 ccatgaccat cggtgctgat ggcgaagtga tgcacagcct ccatgcagat aaaagcggga
     3661 cgattaccgt taaccttctg aagacatcac cgacaaataa aaaattgtcg ctggcgtata
     3721 acgcacagag ccagtcttct gccacatggg ggaataacgt tatcgtgatc cgcaacaagg
     3781 tcagcggcga catcatcacg gcacgcagtg ttgcgttcca gaaacaaccg gataacgcca
     3841 acgctaaaac cggtaatacg atgccgtggg tgtttgactg cggcaagatt gaccaggttc
     3901 tcggggagtt ttaatacatg gaattcgaaa ttaaaggcgt gaaatatcgc gtggcaaaac
     3961 tcagcgtttt tgaccagctg aaagtgaccc gcaaacttct gccggtactg gcgggaatga
     4021 tgtcagattt cgggagcatt cgctcccgtt tgcctgctga cggcaaaatc gaaaccgtga
     4081 aattcgagca gttaaaaccg gtgtttgaaa ccatgctccc gcgtatcgct gaggaactgt
     4141 cttccctgac cgaagatgac accgaagcga ttattcatcc ctgtcttgcg gtggtgtcgc
     4201 ggcgtcatat ggacggatgg gtgccggtat ttaccaaggg cgaactgatg tttgatgata
     4261 ttgacctgct ggtcatgctg cagctggtgg cgcgggtggt cgccgattcg ctgggaaatt
     4321 ttttgcctac accccttacc agcacgacgc agagtctgca acagggctga cgtttaacag
     4381 cctgccggac gggctgtcct accttctcaa tccggttgac gccgggttaa ttccttatac
     4441 agcacttaaa gatggctctg tcgatttgta cgacattgct ctcttgaatg accatctggc
     4501 ggtaaaagcg gataaccagc ggcgcattga gaaatggaga gaggataatg aacgctgaaa
     4561 ctattaaaga tttcctcgtc tcgcttggct tcagtgtgga tgatgcagga gcgaaaaagt
     4621 tcggttctgt cctcgccggt acaactgcaa atgtcatcaa aatggggctg gccgtcgaag
     4681 gaactgcact gtccgtggtg gccttcacgg ctaagatcgc ctccggtctg gataatcttt
     4741 actgggcgtc acagcgcacc ggcgcgacgg tccatggaat tcagtctatt ggctatgcgg
     4801 tttcgcaggt tggcggcagt gcagacgctg cgcgctcttc tctggaaagc ctctcccggt
     4861 ttattcgtaa caatcccggt gcagagggat ttctgaatcg cctaggggta cagacacggg
     4921 atgccagcgg taacatgcgt gacatggcct ctatttttac aggtgtaggc cagaagctca
     4981 gcggcatgcc gtattaccgg gctaaccagt atgcgcagat gctgggcatt gacgaaaata
     5041 cccttatggc gatgcgccgg ggtgtgggtg gcttctccgg gcagtacagc gcaatggcga
     5101 aagctatcgg cttcaatgct gacgaggcgg ccagaagctc caacaaattt atgacctccc
     5161 tgcgcgagtt cggcgcgatg gcaggcatgg cccgtgacaa aatcggctct aatcttgctg
     5221 gtggtctggc gggttcgctg gacacactgc gccgccacat cctcgataac ttcccgcgca
     5281 tcgagcagac cctgacgaaa gccataaaag gcattctggc gctcggagac atcatcgggc
     5341 ggctgttctt caggctaatt gagggaacat ccagccttat cacctggtgg caatcgctgg
     5401 ataagcaaac gcgggagctc atctcgctgt ttggcgcgct gacgattgcg ctgcgcattc
     5461 tgaacagtac gttctggatg tcgccgattg gcctcattac cgcgctggcg gcggggattg
     5521 cccttctgtg ggaggactat cagacctgga aggaaggcgg cgacagcctg attgactggg
     5581 gcaagtggaa gccggaggtc gatgccgcgc tgaagatggt tcgtgacctg aaagggtctg
     5641 ttaatgaact ggtgaaagcg ctggcgaaac tgctcaatat tgaccccaaa tcatggtccc
     5701 tgaagtggga tttcagcaac ttcatcgacc agatgggcga attcagcaaa atgctgaaca
     5761 tgatcgccga cctgctcaac gctatcaaag atggccgctg ggctgatgcc gtcagcatcg
     5821 gcaaacagat acttaatcag ggcagcgaaa atccgtcagc gatgccgatg gttacagaca
     5881 gcgctaacag tactgccgac tggattaaag agcactgggg attcgatccc cgcagtgtgg
     5941 gccggacggt acgcggctgg tttggtgatg atgagccgga acaatatgca caggctacga
     6001 aacgaggaga acggaataac aatccgggaa accttaattt tgctggtcag gcaggggctt
     6061 ctcttgaacg cccgggcggg cgatttgcca gatttgaaac tgcttttgat ggattacggg
     6121 ctcttgctcg tcagttaatg ctgtacgccg gacggggaat aaacagtgtg gagaaaatta
     6181 tctctacctg ggcacctgcg tctgataata acaacacaac tgcgtatatc agggctgtat
     6241 cgcaacgact gggagtggat ccccgggctg ccctgaatat gagcgatccg caaaccatgt
     6301 cagcattgat gagcagcatt atccagcatg aaaatggaag aaatatctat tctcgagagc
     6361 tgattaataa ggctgccgtg gcgggaatta gtggcaaaat gacagaggtt aaccagcaaa
     6421 atacttacca catttacggt ggcggagatc cgcacgctgt cggtaatgag gttgcacgtc
     6481 ggcaacagtc tgcaaatgct caggtcatgc gaagtaatca ggtgagggtg ggttagtgga
     6541 tattctctct acactttttc atcagcagag cagaaaaata ggaatgattg ttcccagtgt
     6601 tgttatttca gagaagcata cagatatgct tgaaataaca gagcatccgg tagaggtcgg
     6661 ggccgctgtc gctgatcatg cctataaaaa accgtcagaa gtggtgatgg aggttggttt
     6721 cgccggtggc ggcgcattgc tggattttgc cagtaatctg acggctacca gccagctcgg
     6781 cctgagtcct cagcagacgt atcaggagct actggatctg caggaaagcc gtatcccctt
     6841 cgatgtggta accggtaaac ggctgtacag caacatgttg atccgggcgc tggaagtgac
     6901 gacggacaag acaaccgaaa acgtcctgtc cgccgtcctc accctgaggg aggtcattat
     6961 ctcccggaca cagcagatta ccgtcgcgga taaaaccaac atgaaggaag gggccagcac
     7021 gtcggcggta cagaacagcg gcaacaaaac cacaaaacct ccagatactt cactgctgaa
     7081 aagcatcacg ggtaacgtgg cgtcattact ggggggcggc taatgacaat tcaggaaatt
     7141 ccgctgacag cggacaacca gcagttcagc atcgtcctgg gtggtgtcac ctggcggatt
     7201 agcatcatat ggcgcgatct gtactggatt atggacctgc agaacgacag aggggagccg
     7261 gtaatctccg gtattcctct cgtcactggt gctgacctgc tggcgcagta cgcctgtatg
     7321 gggcttggtt ttaagctggt ggtggtctgt gatgacaaca cacaggatta ccccacaaaa
     7381 actgacctgg gcggtcgcag ccatttactg gtatcaacgg agtaagcatg tcacagaact
     7441 ggatgagaca tttcgagctg cagcttgtgg acgggaacgg tcagggaatt gagctaagtg
     7501 attttaaagt cacctttacg atcgactggt tcaacatcag cagcgcgtcc cgggtaggga
     7561 ctatcaaaat ttataacctt tcggcagata ctgtgaaccg aattaccggg caggagtttt
     7621 cgaaagtgcg tctgattgcg ggttacgacg gtatcgcgcc ggaggtgtcg gcaagcgacg
     7681 tcgggacagt gcgggaagtt gacgcggcgg acgtgggcca gagtgatggg cgcaactacg
     7741 gactgatttt cagcggtgaa attcgctact cggtcacagg aaaagacagt ccggttgatt
     7801 cctacgtcct gattcaggca gcagatactg atctggcttt tgccaccagt ataacctcac
     7861 agacgctggc tgccggttac acggtcgctg atgtgaaccg tgcgctgatg aaagacttcg
     7921 aagccaaagg tgtgaccgaa ggcctgacgc ctgaaatgcc tgctactgta ttcccccggg
     7981 ggcgggtgct ctttggcatg acgcggcatc taatggataa cgtagccggg caatgtggcg
     8041 caacatggca attcgtggac ggtcagcgcc agatggtggc gactaatgaa tatgttcacg
     8101 aagcgattgt gctcaacagt gctaccgggc ttattggcat gccgcagcag accatcggca
     8161 acggcgtaaa cgtccgcgct cttattaatc cgaacatccg ggttaacggg ctcattcagc
     8221 tggatcaggc ttccgtctat cgtaccgcgt tgtcgaacaa cgatattgcg atggctggtg
     8281 ggcagatcac cgaccagaac acggacggaa atattacgct aagcggcacc acatcgcagc
     8341 ctgccagcat cgcaacggat ggcgtttata ttgttcgcgg gattatgtac actggcgaca
     8401 caaggggcca ggcgtggtac atggatatga tgtgcgaagc gcgtggggct agcgatatac
     8461 ctagccagtt ggcaatgcaa cggggagcgt aagcgtgaaa aaacaggtga tagttgcaat
     8521 aacgatgctg atttctacga cttcttttgc cgaaccaggt aagtttgaat ttaaagcagc
     8581 cgacctgatg gagcagcaaa agtttacgga atggcaacat tacgcatctg tgaagccttt
     8641 tttctcaatg aatgggcgac gttttacaat gaccttagat gatttcgctg acatcatcag
     8701 gaacacattc cagcaatgta atgatttgga cgcatacacc aaccgcaaag gaacccggga
     8761 ttcttgtcag gaatatattt acaacggtat gaaagagtgg ataaaactgt caaaggatcc
     8821 tagtgttagt ccgcaagcat ggaaaattgg tgctagatat gctttcaaca caaataatcc
     8881 tgtccccggt agtaatgtga ttgattttaa tggttgggct ggtggtattc gtgttgcaaa
     8941 atcaaaaggg ttctaatggc actagagtag aaaatcctcg atttttcagg gaaatgggct
     9001 cgtagtaaaa tctagagatt cattttctaa gggggatttt gtgcgaaact actaagtaga
     9061 tcagatgtgg attgactaaa ctgtatacgc gaacataaaa tctgacaaaa aataagtagt
     9121 gagtgaactg tggcgagacc aaaactaaaa gccgaagagc gttttgacca gttgattaat
     9181 gtaatcaaca gtggggagcc acttgatatg tggactttcc gagaagtggt ttcggagtat
     9241 ggttctaatc atactcctat ggctgaagca cttgtagcgt tggcttatat agctaagggt
     9301 gaggtgctct ttgggattga gtcgcttgag gtcatccttc cacatgctga cgttaatttc
     9361 gcacggatct tttgtaaact tctcgagcga ttttctctac tcgaaaaact ggattactac
     9421 atatattctt tagctgataa gtttcctaca aagtggttta cttacaaagc tggcggcgtt
     9481 gcgtacttgg tagggaaact atcgaaatgc gtggagtatt tagggcgtca ttgcaaaatg
     9541 ttatccgaag aagagcatag ggatgacgca gaaaccttct tgcaggaggt aattaacgat
     9601 atggatgaag cttataaaaa ttcagggtgc tcttcagagc aatataggca agttgccttg
     9661 gctgtgcaca gagttatggc tgaattcccg tcaactgaat atcgcgcgga cattaacggg
     9721 gcgtctggtg gaacgtattt ggttgaggtg gttaaggcgt ctccagagcg ggttgttgca
     9781 atgaatatgc gacttgctga tgaaatctgc tcgatcgatt tgcttgatga ttgtaacctg
     9841 atagcaagat tttccgttga aagaaatgga ttaaaagagt gtaaatatgc ctataactag
     9901 ttctgagttt ttgtcttcgg ccgaacgttg ttttgctgaa gattgtgaga ttggttatcg
     9961 taatgctatt tcacgagcat attacgctct ctaccatgaa attaaagata atctaactag
    10021 tcttcctgct tatactcggg atcatcatgc cagccttatc tcttatctta aaaataaggg
    10081 agaaaataaa ctggaaccct atgatcctcg aagtctaaaa tctatggctt acaaacttga
    10141 gcaacagagg ttagctcgga atgaggctga ttatgattta agtagttgtg gcataaataa
    10201 agcaatggca caacaatcaa tcttggaagt aaaaacaatt ttttctcaat gggaacaaat
    10261 gaagactgat gtagccgttt aggtctaact gatttattaa taaaaacccg ccatctggcg
    10321 ggttttttgc tttctggagc ctactaaatg gcagtatctg accagacccg cagcggcgac
    10381 cttgccgaaa cattcaaatc tgaacgggaa acaacaaaga accagatccg tgtcgccttg
    10441 cctggcattg ttcagtcatt cgatcccgac gcggtgacgg cggttgtgca gccagctatc
    10501 cgttcggttg aaaaggataa cgacggtaac cgcattacca aaaattaccc gttgctggtg
    10561 gatgtgccag tggtatttcc gcgcggcgga ggctgtacgt tgacttttcc ggttaaagcc
    10621 ggggatgagt gtcttgtcgt ttttgccgat cgttgtattg atttctggtg gcagagtggc
    10681 gggatacagg agccggtcga tgacagaatg catgatttat cggatgcgtt ttgtattgtc
    10741 ggtccccagt cgcaggcaag gaagattagc ggtattaata ccagtgccac acagttgcgt
    10801 agtgacgacg gcagcaccta ttttgagctt aatcctgata ccaggaaaat taaaattgtc
    10861 gctccggggg ggcttgatgt ggttgcccct ctggctgatt tttctgagaa agtaaccatt
    10921 catggcctgt taacctggat gggtggcatg gtggggtctg ttgtttctgg tgtggcttca
    10981 aaaatcactg gtgctgttga gtttttgggt agcgtgaagg ctaacggcaa gccaatcgat
    11041 gatacgcaca ctcatggtgg tgttcagcgc ggtggaagca ataccgacgg agtaaactga
    11101 tgcgatacag acgtgaagac gccgatggcg attacacctt tggcagcggt gatgacacct
    11161 ggctgattaa ctcaccggag gccgtggcgc aggcggtaaa aacgcgattc gaattgtggt
    11221 atgggcaatg gtttctcgac accaccgagg ggactccgtg gagccagtcc gtgctgggca
    11281 ggcaaaaacc ggaaacttac aacctggcga tcagaaaacg cattctggaa acgcggggcg
    11341 ttaaatcaat cctctctttc aatacgacgg tggataccac gacccgacgt gtcatgtttt
    11401 ccgctgaaat cgacactctc tatggaataa cgactgttac atcggaggcg taatggctct
    11461 gaaccttgat tctctcggtt tatctgcaaa ggtaaccgcg gaggggatca gtgcgcctga
    11521 ttatcagacg atactcagca ccctgattag ctattttcag cagatttatg gcagtgatgc
    11581 ctacctcgaa ccggacagca aagacggcca gatggtggct ctgacggcgc tggcgattca
    11641 tgatgccaat aatatggcga taactgtcta caactgtttt tcaccggcaa ccggctatgg
    11701 ggctgcactg accagtaacg tgaaaataaa tggtatttca cgtaaag
//
LOCUS       DARNVM010000151        11721 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.151, whole genome shotgun sequence.
ACCESSION   DARNVM010000151 DARNVM010000000
VERSION     DARNVM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.151"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1777
                     /locus_tag="SI621_003749"
     CDS             <1..1777
                     /locus_tag="SI621_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HEI4066411.1"
                     /translation="ELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGT
                     GAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPV
                     ILQERCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKI
                     FATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTHG
                     HMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANASDEPHIEQLVGECR
                     VMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQ
                     LLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEARE
                     ILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDI
                     ELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEV
                     IDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKK
                     EVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1817..2296)
                     /gene="xdhC"
                     /locus_tag="SI621_003750"
     CDS             complement(1817..2296)
                     /gene="xdhC"
                     /locus_tag="SI621_003750"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HEI4066412.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2293..3171)
                     /gene="xdhB"
                     /locus_tag="SI621_003751"
     CDS             complement(2293..3171)
                     /gene="xdhB"
                     /locus_tag="SI621_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HEI4066413.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3182..5479)
                     /gene="xdhA"
                     /locus_tag="SI621_003752"
     CDS             complement(3182..5479)
                     /gene="xdhA"
                     /locus_tag="SI621_003752"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HEI4066414.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVHVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5893..6648
                     /locus_tag="SI621_003753"
     CDS             5893..6648
                     /locus_tag="SI621_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEI4066415.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6727..6800
                     /locus_tag="SI621_003754"
     tRNA            6727..6800
                     /locus_tag="SI621_003754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6759..6761,aa:Gly,seq:ccc)
     gene            complement(7116..8345)
                     /locus_tag="SI621_003755"
     CDS             complement(7116..8345)
                     /locus_tag="SI621_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEI4066416.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            8600..9781
                     /locus_tag="SI621_003756"
     CDS             8600..9781
                     /locus_tag="SI621_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEI4066417.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            10068..10904
                     /gene="kduI"
                     /locus_tag="SI621_003757"
     CDS             10068..10904
                     /gene="kduI"
                     /locus_tag="SI621_003757"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEI4066418.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            10934..11695
                     /gene="kduD"
                     /locus_tag="SI621_003758"
     CDS             10934..11695
                     /gene="kduD"
                     /locus_tag="SI621_003758"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEI4066419.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         2990 a         2711 c         2991 g         3029 t
ORIGIN      
        1 ggagcttgcg actacgcagt cagtattgat gcaaattcaa ccgacaattc agcgttttgc
       61 cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa acttgtgtcg
      121 cgtcgccgga acgggcgcgt atgggaagtt tcttggccgc cagttgagcg gcaactcacg
      181 cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt ctcgcttcga
      241 tcccctttgt gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat ttctgggtac
      301 gcctgtcatt ttacaggaac gttgtgttgg ggtgataagt ttgattgccg ttacccacga
      361 gcaacaagaa catatcagtg ataatttacg cgaattttcc gattacgttc gccatatatc
      421 caccattttt gtttctaaac ttctggagga tcagggacca ggagataaca tcagtaaaat
      481 attcgcgacc atgatcgata atatggatca gggcgtgtta gtggttgatg ctgataatcg
      541 ggttcagttt gttaatcaga cagctttaaa aacactcggt gttgtacaaa ataatattat
      601 tggaaaacct gttcgtttca gaccattaac atttgagagt aattttaccc atggtcatat
      661 gcaacatatt gtttcgtggg atgataaaag tgaattaatc attggtcagt tgcataacat
      721 ccagggccga caactatttt taatggcgtt tcaccaatcg cataccagtt tttctgtagc
      781 aaatgcatct gatgaaccgc atattgaaca attggttggc gagtgccgtg ttatgcggca
      841 attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg tggttggtga
      901 aagcggcacg ggtaaagaag tcgtcgcccg tgcaatccat aagttgagcg gaagacggaa
      961 taaacccttt attgctatca actgtgccgc gattccggaa cagcttctgg agagcgaact
     1021 gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa cagggttgat
     1081 tcaggcggcg aatacgggca cgctgtttct cgatgaaatt ggtgatatgc cattaatgtt
     1141 gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg gtgccagtag
     1201 cccaatacaa gtcgacatcc gcatcatttc tgcaactaat cagaatttgg cccagttcat
     1261 tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc cgataactct
     1321 tccaccgctg cgtgaacgtc aggaagatat tgaattactg gtgcattact ttttacatct
     1381 gcatacccgt cgtctgggat cggtttatcc tggcattgct cctgatgtcg tcgaaatatt
     1441 gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg aatatctggt
     1501 taacgttgtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa atctgctgaa
     1561 taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc tgtcactcga
     1621 tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg aggcgctttc
     1681 acgtcataac agcaagaagg aagttgctga tgaactgggc atcggcattg ctacgctcta
     1741 tcgcaagatt aagaaatatg agttgttaaa cacataacct ctgagaatcc ggcctgaatt
     1801 caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt acaatcatct
     1861 gataccctgt gcagcgacaa agatttcccg ccagtccacg acgaatttcc gtaatggtta
     1921 atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg cccggcgtac
     1981 aaaacccgca ttgcactgca ccagatttcg cataagccag ttggacatga gaaagtttac
     2041 cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag gccgcaagga
     2101 ataagcaact gtctatcgca gtgccgtcga ccagcaccgt acaggcaccg cattcgccta
     2161 cgcagcaacc ttgtttgaca ctaagaagcc cttgttctcg gagtagttcc gaaagcggca
     2221 ttcctggcgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg atggtaattg
     2281 tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc taatcacttt
     2341 tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc atgaagaacg
     2401 cggggcgaca tcttgcagga ctgattcgct gatggcttcc agcgtttgca ggtttaatgg
     2461 cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc
     2521 aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc
     2581 aattgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg
     2641 ttctttcggc tgtggcggaa aatgaaaggc gacaaggatt tcgtcatgct caagagacac
     2701 tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc gtggggagtg
     2761 gagctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc tggtggcacc
     2821 gttgcaaata tttccgccgt aggtggcgac attacggatc tgcggcccgg cgattgatgc
     2881 agccgcagca cataacgccg ggagattgcg ttggattacg ggatcttcaa tgagctgagt
     2941 aaatgttgtc gcggagccga ttcgcagcgc gccatcttcc gcctgtgtta ttccctgaag
     3001 ctctgccaga ttgtggatat caacaatatg gcgatagcgg tcattgtgat ggtgaagctg
     3061 tatcagtacg tcagtgccac cggcaagcaa tttggcctgc ggattgtcag ccagcagggt
     3121 gatggcatcg gcaagggtgg ttgcgcgatg gtaagaagca aaatcaaaca tgatgttatc
     3181 ctcaaatcaa tcctgccaga tggaattctt catataatcg ttttggcgtt agcggcagtg
     3241 tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg gctacaggaa
     3301 ttatgggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca ttgatttcga
     3361 cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa tccagcagat
     3421 tggggttacg gaccacgccg cttttcgcat cgatgatcat ctcttcaaat agcgcccagc
     3481 caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagcgga ttaagaatat
     3541 ggcccgaatc atgaacgttg aggatgcggt tgatggtgac tttacacagt gcaatatcga
     3601 ccgtcagatc aacaaaggta cagccaaacg ccggtgggtt agtggtggtt ttgatggagc
     3661 tttcggcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt
     3721 ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct tttatcaggg
     3781 tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt ttctctttta
     3841 ataacagtgc cgcactgcgc agcgcaggcg cggcaacata gctctgacgt gaggcaaatg
     3901 cgccggggtc gaatggtgta acgtcggtgt cttgggttga aataacgtga acatcgctga
     3961 ccggaactcc cacggtttct gccaccattt gtgagaacac ggtgtcggca ccctggccga
     4021 tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaac aggcgcgcgc
     4081 ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa
     4141 cgccacgacg taaattgcct tgctggttct ggcactctgc acggcgtttt tcccattcaa
     4201 agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata cgttttcccg
     4261 tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa
     4321 tacctaacgc tgtcgcggca tcatcaagca tagactcaac ggcaaatacg acttgtggcg
     4381 cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc tttgaactgt
     4441 aagcgtaggc acaacgagga taaaggtaag cgactttatt ccccccagca gaagcaatgg
     4501 agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta taacctttca
     4561 atgttccgtc gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg cgggtacggg
     4621 ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg ccaagtttgc
     4681 tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca aaaccgccac
     4741 cgataaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc tgaccaacca
     4801 cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc gagtcatcct
     4861 ccatccatgc cagcgatgtc acgctttcca tatgacaatg ttgaataacg ggagtctgat
     4921 agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta cccgtcgaca
     4981 tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct tctgccagcg
     5041 ccgcttctgg cgaggtgata acgggtaatt cttgccactc aatgctgacc aattgcgccg
     5101 ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtcg ccatgatgac
     5161 gaacatggcg cgttagcagg gcacgatcgg cggtatcgcg cttgttttcg tcaagtgtcc
     5221 aggcatgccc tgccgtggcg aatgggattt ctggcacatc ttcccaggta aaaatcgcca
     5281 ggacgcccgg caaactcctg gcttgttcat cattaatact tacggcataa ccatgtgcga
     5341 tagggctacg tacatatttc gcgtaacaca tgcccgccat aacataatcg tcagtatatc
     5401 gtgctcgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca cccgtagcgg
     5461 ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct tatggcaaag
     5521 atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttcg gggaggaagg
     5581 aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg aatagggaca
     5641 gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattagga ttattacgca
     5701 tcaaggtggg aaatagggta aaagtcagga acttcgatac cactacagca aatattttgc
     5761 tgatgatact tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca
     5821 gaagcaagag attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag
     5881 cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg
     5941 ttatcggttg ggctgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg
     6001 tattccggct ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc
     6061 acgggaacca gcgtaaaaga actggcgcga ctgaacggca tttccccccc ttacaccatt
     6121 gaagtaggtc agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca
     6181 accgccaaat caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct
     6241 tcctggccgc cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg
     6301 ccgtattcga cagcagatgg cggcaataaa gggattgata tctcagctcc acgaggtaca
     6361 cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg gtaaccagct gcgtggctac
     6421 ggtaatctca tcatgattaa acacagtgaa gattacatta cggcttacgc tcataatgac
     6481 acgatgctgg taaataatgg gcaaagcgtg aaagctgggc aaaaaatcgc cactatgggg
     6541 agcactgatg cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt
     6601 gatccgctac gttacttgcc gcctcagggc agcaagccaa aatgctgatg gtgaattaat
     6661 cagcggtcag cagcgtggta cttgctaagg agagcgtaag gtttataatg ccttacgcat
     6721 ctcgaagcgg gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc
     6781 gattcccttc gcccgctcca atttatcttc acatcatcta cctgccattt ttgttgtatc
     6841 tgattttcta tattatctta attcaagcgc ttcagacttt tcgcaatcgc cgtaatactt
     6901 ccccattttt tgtaaataaa aagcgtttac gcaatgcaat cgtcatgtat cttaaatagt
     6961 cttatttaag atgttcagaa ctctttgtgt gtttaactag ctgataatta tataatatta
     7021 cgagagaagt gatagggcgg aataataaca aatgaacatt atgaaaactg ggatggttca
     7081 aaattgaagc aattttgaac catccacaac ggatattagc taaagaacat taccgatacg
     7141 cacaggatac ccacgatcag cgtaatcaga tttccgatgg aacgccaggg tttaagcgcc
     7201 gggatgagat acgttgacag cgtaggcatg atgaaaagta tcatggcaat gagcgggccg
     7261 ctgatcgcgt aaatcatcga aatcgcgttc gggttaatgc aacaaacaat gaaggtaatc
     7321 agcgatacca acataattga tagtgcgcgg ttaaatgcac gacttttctt tacaccaacc
     7381 tgctgtaatg ttgttttgac aacctctgtg gcaccttcaa taacgccaaa gtacgtaccc
     7441 aggaatgatt tcgacatcgc aactactgcg acaataatcc cggaaattga caaccatgct
     7501 ggggcgttcg gcagcataga aagcgccgat aaaatggtaa ccccttcttc tttagccgct
     7561 tcaatatacg aaggtggaat agaaagcagg cagctaaaga caaagaacaa tacacttatg
     7621 cagatgatga gataagcgac tttcataatt tttttgcatt tatccatagc atgttcgcca
     7681 tatttttcac gtctgtctat ggcaaacgta gaaataatgg gcgtatggct aaaagcgaaa
     7741 accatcacgg gaatcgatat ccatatctgg tgaagggtat tctgattgaa ctctacctgg
     7801 gttgttaata gatcaggttg ccaactaccg accaggtaaa tagaaagaaa taagaaatag
     7861 gcaatcaatg ggaataccaa aaatcccatt acccgaatgg ttgcatggcg tcccatcaga
     7921 aaaatgagat tcaggattaa tacaacaccc agactcacta gcatacggat gcgaagatca
     7981 ataaccatat gctttgccag ctgttccgta agtgagttgg taattgccac tgcatatatc
     8041 aacacgacga caaaaaaggc gatgaagtac agcgtggtaa tcagattacc aatcttcttg
     8101 ccatagtagt gtgttaccgc gcccgttatc ccttcacctg ctgatgtttt cgatgagagg
     8161 atgaactggc ataaggcttt atgtggccaa tatgttaaag gccaggcgac cagagcagta
     8221 ataaacagga ccacagcccc cgccgaacct aactgaatag gaaggaagag cgtgcctgca
     8281 ccaacggctg tgccgtagag cgcgaaactc cacagagttt cttcttttga ccaaatatta
     8341 gacattatct gaacgtatca tctacaaatt aaacaaaatg gagggcaatt taccataaac
     8401 caggttaagt atgggagatt aacgccttat ctacctctct attggtgggt tagtggttgc
     8461 aaaccttacg tgccagttat gtctctgact actactcagg cgtatttcct ttgtgataaa
     8521 aatcaccttt tttaaacaat taagataact gtaattaaca atcatttact attgcactgt
     8581 taattggtgc gaggttgtga tgaaagacgt tgtgattgtc ggggcgttac ggacacctat
     8641 cggctgcttt cgtggtgcgt tagcgggtca ttccgccgtg gaacttggca gcctggtcgt
     8701 caaagcgtta atagaacgta ccggcgttcc tgcatatgcg gtggatgaag tgattcttgg
     8761 tcaggtgttg actgcagggg cagggcagaa tccggcaagg caatcggcta ttaaaggtgg
     8821 tcttcctaat agcgtttctg caatcactat taatgacgtt tgtggttccg ggcttaaagc
     8881 actgcatctg gctactcagg cgatacagtg tggcgaggct gatattgtca tcgccggtgg
     8941 ccaggaaaac atgagccgcg caccacatgt tctgactgat agccgcaccg gcgcacagct
     9001 tggcaatagc cagttggttg atagtcttgt gcatgatggg ttgtgggatg ccttcaatga
     9061 ttatcatatt ggtgtcaccg ccgaaaatct ggctcgcgaa tatggcatca gccgtcagtt
     9121 gcaggatgct tacgcactta gctcgcaaca aaaagcgcga gcggcgattg acgccggacg
     9181 atttaaagat gagatcgtcc cggtaataac ccaaagtaac ggtcagacgt tggttgttga
     9241 taccgatgaa cagccacgca ctgacaccag cgcagaagcc ttagcccgtt taaatccttc
     9301 atttgatagt ctcggttctg tgacagcggg taatgcatca tccataaacg atggggcagc
     9361 tgcagtaatg atgatgagcg aagccaaagc acgagcgttg aatttacccg tgctggcccg
     9421 catccgcgca tttgccagcg ttggtgtgga tccggcattg atgggaattg cgccggtgta
     9481 tgcgacccgt cgttgcctgg agcgtgttgg ctggcagttg gctgatgtcg atcttatcga
     9541 ggctaatgaa gcgtttgctg cacaggcgct ttcggttggc aagatgcttg aatgggatga
     9601 gcgtcgggtc aatgtcaatg gtggtgcgat cgcactcggt catccgatag gcgcttccgg
     9661 ttgccgaatc ctggtttctc tggttcatga aatggtgaaa cgtaatgccc gcaaaggact
     9721 ggcaacgctt tgtatcggcg ggggccaggg tgtggcattg accattgaac gtgacgagta
     9781 gctctgcatt tcctccttac cgataaatat ctgccgtcat taatctgttg aataatgatg
     9841 gctggtgagt cgacatacct tttcagggct ccctgatttt tccattctca tgacatattc
     9901 gtgatcgaca ctgcactttt tttaatcctt aacttgaaaa tgaaacattg ttttattttt
     9961 aattgaaaac cctatcccat gggcatatca tcccctcata gagaaaacga aacagtgttt
    10021 cattgttgtt ttctattttc acgcttcact gattatcgga ggttgatgtg gacgtaagac
    10081 agagcatcca cagtgcgcac gcaaaaacgc tggataccca ggggctgcgc aatgaatttt
    10141 tggttgaaaa ggtatttgtc gccgatgagt acaccatggt ttacagccac attgaccgta
    10201 ttattgttgg cggcattatg ccggtaacta aaacggtttc cgttggcggg gaagttggta
    10261 aacaactcgg cgtaagctat ttcctcgaac gtcgcgagtt aggtgttatc aatattggcg
    10321 gtgccggtac gattactgtc gatggtcaat gctatgaaat cggtcaccgc gacgccctgt
    10381 atgttggtaa aggtgcaaaa gaagttgtct ttgccagtat tgataccgcc actccggcga
    10441 agttttatta caattgcgca cccgcgcata cgacgtatcc caccaaaaaa gtcacaccgg
    10501 acgaagtatc tccagtcacg ttaggcgata acctcaccag taaccgtcgc acgattaaca
    10561 aatattttgt cccggatgtg ctggaaacct gccaattgag tatggggctg acggagctgg
    10621 ctccaggtaa cttgtggaac accatgccgt gtcacaccca cgagcgccgg atggaagttt
    10681 atttctattt caatatggat gatgacgcct gcgttttcca catgatgggg caaccgcaag
    10741 aaacgcgtca cattgtgatg cataacgagc aggcggtgat ctccccgagc tggtcgatcc
    10801 attccggcgt cggaaccaaa gcttatacct ttatctgggg catggtcggt gaaaaccagg
    10861 tttttgatga tatggaccat gtggccgtta aagatttgcg ctagttgtgg gcataaacga
    10921 ataaggtatt gttatgattt taagtgcatt ttctctcgaa ggtaaagttg cggtcgtcac
    10981 tggttgtgat actgggctgg gccaggggat ggcgttgggg ctggcgcaag cgggctgtga
    11041 cattgttggc attaacatcg ttgaaccgac tgaaaccatc aagcaggtca cggcgctggg
    11101 gcgtcgtttt ttaagcctga ccgccgatct gcgaaagatt gatggcattc cagcactgct
    11161 ggatcgcgcg gtagctgagt ttggtcatat tgatatcctg gtgaataacg ccggattgat
    11221 tcgccgcgaa gacgctctcg aattcagcga aaaagactgg gacgatgtca tgaacctgaa
    11281 tatcaagagc gtattcttca tgtctcaggc agcggcgaaa cactttatcg cgcaaggcaa
    11341 tggcggcaag attatcaata tcgcgtcaat gctctccttc cagggcggga tccgtgtgcc
    11401 ttcttatacc gcatcaaaaa gcggtgtgat gggcgtgacg cgattgatgg cgaacgaatg
    11461 ggctaaacac aatattaatg ttaatgcgat agctccgggt tacatggcga ccaacaatac
    11521 tcaacaactg cgggcagatg aacaacgtag cgcggaaatt ctcgaccgta ttccagctgg
    11581 ccgttgggga ctgccgagtg acctgatggg gccggtggtg ttccttgcct ccagcgcttc
    11641 agattatgtg aatggttata ccattgccgt ggatggcggt tggctggcgc gttaattcat
    11701 tcctcttact tttatgatcc t
//
LOCUS       DARNVM010000152        11706 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.152, whole genome shotgun sequence.
ACCESSION   DARNVM010000152 DARNVM010000000
VERSION     DARNVM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.152"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..237
                     /locus_tag="SI621_003759"
     CDS             <1..237
                     /locus_tag="SI621_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEI4066420.1"
                     /translation="KGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELEN
                     AVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
     gene            complement(297..1640)
                     /gene="gdhA"
                     /locus_tag="SI621_003760"
     CDS             complement(297..1640)
                     /gene="gdhA"
                     /locus_tag="SI621_003760"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEI4066421.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMHVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            1876..2148
                     /locus_tag="SI621_003761"
     CDS             1876..2148
                     /locus_tag="SI621_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HEI4066422.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2114..2521)
                     /gene="nudG"
                     /locus_tag="SI621_003762"
     CDS             complement(2114..2521)
                     /gene="nudG"
                     /locus_tag="SI621_003762"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HEI4066423.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            2608..3228
                     /locus_tag="SI621_003763"
     CDS             2608..3228
                     /locus_tag="SI621_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEI4066424.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3237..4544)
                     /gene="ynjE"
                     /locus_tag="SI621_003764"
     CDS             complement(3237..4544)
                     /gene="ynjE"
                     /locus_tag="SI621_003764"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HEI4066425.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKANAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSNSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4611..5264)
                     /locus_tag="SI621_003765"
     CDS             complement(4611..5264)
                     /locus_tag="SI621_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEI4066426.1"
                     /translation="MLCVKNVSLRLPESRLLKNVNFTVSKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     VTLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5264..6799)
                     /locus_tag="SI621_003766"
     CDS             complement(5264..6799)
                     /locus_tag="SI621_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4066427.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQTL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIVFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(6772..7938)
                     /locus_tag="SI621_003767"
     CDS             complement(6772..7938)
                     /locus_tag="SI621_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEI4066428.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(7948..8496)
                     /locus_tag="SI621_003768"
     CDS             complement(7948..8496)
                     /locus_tag="SI621_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEI4066429.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8496..9203)
                     /locus_tag="SI621_003769"
     CDS             complement(8496..9203)
                     /locus_tag="SI621_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEI4066430.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGALWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSILTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(9218..9895)
                     /gene="ydjY"
                     /locus_tag="SI621_003770"
     CDS             complement(9218..9895)
                     /gene="ydjY"
                     /locus_tag="SI621_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HEI4066431.1"
                     /translation="MLQHYSASWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVAGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(9900..10610)
                     /locus_tag="SI621_003771"
     CDS             complement(9900..10610)
                     /locus_tag="SI621_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEI4066432.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(10777..11583)
                     /gene="xthA"
                     /locus_tag="SI621_003772"
     CDS             complement(10777..11583)
                     /gene="xthA"
                     /locus_tag="SI621_003772"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEI4066433.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSTLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2855 a         3096 c         3034 g         2721 t
ORIGIN      
        1 aaagggttag tgcagagcat gtcgcgcaag ggcaactgtc ttgataatgc ggtaatggag
       61 aatttctttg gtcacctgaa agaagaaatg tactatcgcc gggactacag aagtgtagaa
      121 gagctggaaa atgccgttaa cgaatacata acttactgga atcaaaaaag aataaaactc
      181 agtcttgggg gactcagccc ggtagaatac cggactgagt atcaaaaagc cggttaactg
      241 aaattgtcca ggttttgggg ttcagttcag tttgcgctgt atccggcaca attgtcttaa
      301 atcacgccct ggcttaacat cgcatccgca accttcacaa aaccggcaat gttcgcaccc
      361 tgcacgtagt tggtttgctc accttcacca ccatgctcaa cacaggcatg gtggatatcc
      421 agcatgatgt gatgcaaacg cgcatcaact ttctcggctt tccaccccag gcgcgcagcg
      481 ttttgcgcca tttccaggcc cgatgttgct acaccgccag cattagccgc tttacccggt
      541 gcaaacagta cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt cggcatattt
      601 gccccttcgg cgacggcttt aacgccattg gcgataagct gatgcgcggc gtctacatcc
      661 agttcattct gggtggcgca aggcagggcg atatcaaccg gtacagacca cggctgttgg
      721 ccttcgagat agaccagacc aaattctttg gcgtaatctg ccactcggcc atcgcggctg
      781 gctttgattt cgataagacg tgccagtttc tctttcgtga atccgctttc atcaactaca
      841 gtgccactgg agtctgacgc agtgatcaca cgagcaccaa attccatcgc tttttcgata
      901 gcgtactggg cgacgttgcc ggagccagaa acggaaacgt gcattccttc aaaacccata
      961 ccgtggcgtt ttagcattgc ttctgtgaaa taaaccagac cgtagccggt agcttccggg
     1021 cgaataagac tgccgccaaa tgaaaggccc ttaccggtga agacgcaggc ggtattgttg
     1081 gagagctttt tcatcatccc cgccataaag ccgacttcac gaccaccaac cccgatatca
     1141 cctgccggaa cgtcggtatc cgcgcccagg tggcgataca gttcagtcat cagcgcctgg
     1201 caaaaacgca tcacttcacc ttcgcttttt cctttcggat cgaaatcgct gccgccttta
     1261 ccaccgccca tcggcagagt agtcagggca tttttgaagg tttgttcgaa gccgaggaat
     1321 ttgagaatgg aaaggttaac tgacggatgg aagcgcatac cgcctttgta cgggccgatg
     1381 gcagagctga actgcacacg ccatgcacgg ttgacctgta cctggttgcg atcatcaacc
     1441 cataccacgc gaaactggat cacgcgctcc ggttcaacca gacgctccag taatgacatc
     1501 tggcgatatt ttggattttg ttcaagaaaa ggccagagtg tggtcattac ttcacgaacg
     1561 gcttgcgcga actcggtttg attcgggtcg cgcttttgga catggttgag gaatgactcc
     1621 agagaatatg tctgatccat agatataaaa cccttatata ttaatacgat tgtgcttatg
     1681 ttgtttatgt catgtgcttt tgcagttttc gactatacca tcaagaatgt gacggtaagc
     1741 aagaaaaaaa tgacccagga aagcaaatta ataacgagag taatctcata accaaatgcg
     1801 ataactcaga cgctaaagtt ttgccacccg gctaccgtta tcagtagcag agacaataac
     1861 gttaaggagt tccctgtgag tcgagcattg ttcgccgtag tattagcatt tccattaatt
     1921 gcactggcta acccgcatta tcgtccggac gtggaggtaa acgtaccgcc agaagtgttc
     1981 agttcaggcg ggcagtcagc gcagccctgt actcagtgct gcgtttatca ggatcaaaat
     2041 tattctgaag gggcggtaat taaagccgaa ggaatactgc tgcaatgcca gcgtgatgac
     2101 aaaaccctta gcactaatcc gctggtctgg cggcgcgtaa agccataaac gcctctaata
     2161 gtggaatgtc agcaggggcc agcggatatt gcagcgcctc ttcaggtgag caccagacca
     2221 gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa
     2281 gatggataat ccgccccgaa acttctcgct gatggctggc aacatattca cccacagctg
     2341 cttcgatgcc cagttcttcg cgtaactcac gcaccagcgc ctgccgctgg ctttcatccg
     2401 gctcgacttt accaccggca aactcccata atcccgcctg atcgctatgg gcggggcgtt
     2461 gcgcgagtaa aattttgcca tcacgttcga tgatggcggc aacaacttca atcattttca
     2521 taaattacac acgggcagat aagcgaaaaa tgccataata tccgcactgt tgctcaatca
     2581 gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa
     2641 ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg gcattacgcc agatggttta
     2701 acattagtgg gatttgccat cggtgtgctg gcactgcctt ttctggcgct gggctggtat
     2761 ctggcggcgc ttgtcgtcat tttgttgaac aggctgcttg atggtctgga cggtgcgctg
     2821 gcgcggcgca gagggcttac cgacgcgggc ggctttcttg atatttctct cgattttctt
     2881 ttttacgcgc tggtaccttt tgggtttatc ctcgccgcac cgcagcaaaa tgcgctggca
     2941 ggcggctggc tgttgtttgc gtttatcggt acgggcagca gttttctcgc ctttgctgca
     3001 ctggcagcaa aacatcagat tgataacccc ggttatgcac ataagtcgtt ttactatctg
     3061 gggggattaa ccgaaggcac tgaaacgatc ttactgtttg tgctgggatg tttattccct
     3121 gcatggtttg cgtggtttgc gtggatcttc ggtgcgttgt gctggatgac gacctttacg
     3181 cgggtgtgga gtgggtatct gacgctgaag tcgctccagc gtcagtaatt gcaatgttat
     3241 ttgctgctgt ccgggccgcg ttcaccggtt gcaaccggat tttttggatc gctgctccat
     3301 tcgtaccagc cgccgtcgta aacggagacg ttattccagc ccattgcgcg tgcgtacata
     3361 aaggtttcgg acgcgcgcca gccagtgccg cagtagaatg aaacttgctg atctggtttg
     3421 atattccatg ctttccacat agcggtaata tcatcggcgc tgcgcatggt gccatccggg
     3481 ttatggaaat cttccatatg cgtcgagttg ctacctgcgt gtccccaacg tgctccggct
     3541 atttcacctt ttggtttaat atagctgtaa ccgctggtcg taccgataaa ttctggccac
     3601 gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca gtccacgcgc ctgttccata
     3661 tcaagcatca gttgcggttg tgccgggatc ttcacgccga aatccggttc cgctttcact
     3721 ttcggtggcg ttccgcgctc aacgggcagt cccgcgtcgg accaggtttg ccagccgccg
     3781 tccagcaggc ggacatcttt cacgccagcg tagagcataa tttgtgccac acgcgctgca
     3841 gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat ggcgaatgcc gtgttttgcc
     3901 agcatcgctt ttagttgttc atcagaaact ttgttccaca gcggttcgct ttccacttcg
     3961 ttggtatcga tgtagtcagc gccgggaata tggctgataa ggtaaagctt aggagcgccc
     4021 caggccgctt caatgacttt ccagtcaccg gcaggtttcg ccgtaacctc tttaccttgt
     4081 tgcaggtcgt gcagccattg cggataaacc agctgctcaa agtgcggcag tttttgcaga
     4141 cgggaaggtt cgcttagcgc gtcactcaga atggagatat gcgtaaaacc tgctttttgc
     4201 agtcgcgttt tgacggcgtc gacatctttg ccattaccgt atagcgccac cggggcattg
     4261 gctttcaggt tatgttgctt gatccactcg ttgagctgtt cggtgctcat tttgtcgagc
     4321 cagctggcag agaggtttaa ggcggcaggt tcatgaccag aagggccatt taaggtttgt
     4381 ggccagccgt tataaaacgc gctggggcga gtatctatcg ctttgccatt ttgttgttga
     4441 agctggtcaa gtgtaagagg cttcgccagt tcagcggccc acgaagaagc gcaagccagc
     4501 cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt tcatcgaaaa tcccgacatt
     4561 gtagttttga gtgcgccatt ctgaactgac gcattgggaa aaactttgcg ttatcgcagt
     4621 ttgttgtaat tttctgacca ctgcgccata tccagaacag aactatcagc aggaacatcc
     4681 tggagatcgt gcgttacctg aacgactggg atcgcctggg cgcgaacttc gctgaacacc
     4741 cactggcgaa aattatcgcg cagagccaca tcaagacggc tgaatggctc atccagtagc
     4801 aacgcttttg gctgagcgag aagagcgcga agcagagcaa cgcgcgctcg ctgaccgcca
     4861 gacaaagtgg caggatcctg atggaaagct ccgtcaaggc ctgaacgctc aagtgcatca
     4921 ttcacggcat tacgtcgggc attcccctta agtgtcaccg gtagcgccag cagtaaattt
     4981 tgcccgacgc tgaactgatc aaataacagt gcatcctgga aaagaatgcc aatctgacgc
     5041 tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc atagctcacc tgtacaagaa
     5101 aactgtccgg ccagcgcacc aatcatccat gaaaacagag tggattttcc acagccagac
     5161 ggccccatta acgtgacaat gtcaccttta ctcaccgtaa agttaacgtt tttcagcaag
     5221 cggctttctg gtaaacgtag cgaaacattt ttcacgcaga gcattagcgg agtccttgtc
     5281 tgacataacc tacccatttt gcgactaacg cggtcagggc aaaaataata agcggcaaca
     5341 gtagttgcca taaagcctgg gcggcgagaa taccgttgct gccgccgctg cttaatgcca
     5401 ccgcttcagt ggtgagcgtc ggaaaacgcc ccgcgcccag ccacagcgtc ggcatatact
     5461 gtgcaatact gactgaaaat cccaccgcga agacaatcag cgcagggcgt agcatgagtg
     5521 ggcatttcac gtaaaagaag attttggccc gcgaccagcc cagtgtttgc gcaatcaaaa
     5581 ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat aaacagcatc cacggcatta
     5641 cccacagcag atgcccccag accaccgccg tccagcttcc atccaggttc agccatagcg
     5701 ccagcgtgta ctggcccgcc accagcggca gagcgggtaa taaaatgggc aaccatagcc
     5761 acaactggcg acgctgtggc ccccattcca gccacagcag taacaggagc aaagcgatga
     5821 atgccgccac cagccccatt gtcaggctgt tgatgagcgc ttcactattg atcgtcgact
     5881 gatccgcgag gatcgccagc agaaccacac acagcacacc ggttaagggt aaaaaactcg
     5941 ccagcgtatt gcctggcaat aaaggcgtgg caggcttacg aacgccatct acgcggggaa
     6001 tagtacgccg ccagctacgc catagcagat agcccagcaa aacgtaggcg gcgagtaaca
     6061 gcatcagcag caggctggcg agtgcgcctt tcgtttgttg atcggcgtcg ccctgagtta
     6121 accactgcca gctaattact gccagcgtcg ggggattacc tggcccaagg ataatcgcca
     6181 catccacgac cgacagcgac caggcgacaa tcgccagcat cgccattgcc agtgcaggtg
     6241 cgacggaggg caacaacagc cagttcaggc attgccagcg gctgtagccc agcgaatcca
     6301 gtacaataac ttgctgcaac agccgttttt cgctcaatac tgccgctaag atccacagca
     6361 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatgccaaat cggtccattg
     6421 gcggagtgaa atacgggaaa tagtcataaa gcagccctcc gtcagcaaag agcagaaggg
     6481 cgctggtggc aaaagccaca tggggaatgg cgagcagcca cggcagacgg gcgcacatac
     6541 gctgccattt cggcccaggc cacagcgcca caatcaccag cagggcaatc aacaacgccc
     6601 cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa tgcctgcggt aactgtggat
     6661 cggcaaataa cgtctgccag tgtgtcaacg acaaagcagg cgatatcagc gtgagagctg
     6721 ccgggatcag cggtgcataa atcaccgcca ccatcgccca cagcaaaaag attaatgcgt
     6781 accgtaacgg tgtagccatt cttgttccag cgcatttacc caacctgcgt gcggttcagc
     6841 cagtaccggc ggcagatcct gcggcattct tgattgcaat atttcgcgct gcccgtcagg
     6901 cagtttttgc ggatcgagaa ccgaaggatc gccccagaca gcagggtctg ctttacgcag
     6961 ttgtgcatcg ggtgagagca ggaaattggc aactaccttc gccgcagcac tggcattcgc
     7021 gttggcggga atggtgacga aatgcacgtt gccaatcatt ccctcgctaa agccaaagct
     7081 gtaactgctg gcgggtaaat cgccgctggc gattttttgc tgcgcatgcg cgggattaaa
     7141 ggtcagcgac aggcgcaatg tgccggcttt cagcagagca tccatccgcg cgggcgaagg
     7201 cgggaaatcc tttccttcgc gccacaaata cggatgcagc gcatcaagat attgccacaa
     7261 gggagcagtg acacgggcga aagtcgcatc gtccggcgct tcttttaatg ctgtgggatc
     7321 tggcgttagc ataatcagca actgttcaag aaacgccgtg ccggtaaagt ccggtgggcg
     7381 cggataggta accgtaccgg gattggcttt agcaaactcc agtaaggctt gcggcgtttg
     7441 tggtggctgc ggcgtaacat cgcggcgggc gataaacgtc agttgcgcgc ccccccaggg
     7501 cgattcagcc ccttctgtag gcactgaaaa atcttcccgc accggcagct gtgtgtcgac
     7561 atagcgccag ttgggcagag tctccgccca gcccgtttgt aataaattgg cctcttttaa
     7621 ggtgcggaag ttttcgccgt tcacccagag cagatccacc gagccgcctg ttttgcgtcc
     7681 ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg gcatccgcga gacgaacaat
     7741 cttcaggttt atagcgtaat gggttttcat ctcgccgcta acccagtcga gatagcggtt
     7801 aattgcggta tcgccgcccc aggcgttaaa ccagacggtt tgtcctttgg cctcattttt
     7861 tatttcctgc cagtcactgg catgtgttgc caaagaaaac agcgataaca atcccagcaa
     7921 ccacccacaa tggcgcatca tctctcctta agaacgtttt tcaggaatac ggcacagccc
     7981 ctgagcggga atgtccatgg cagaattaaa acgggctgac aggttttgca ggccgatcaa
     8041 tgccgtcagt tcggtgagcg cctgggcgtc aaaatgcgca gccagtcggg tacgcagggc
     8101 atcatcgacc gttggcggcg ttacgcttgc ggcttcggcg tactccagcg ccagccgttc
     8161 ttcatcgcta aagagcgggc tttgccgcca gtcagccact gccagcagtt tatcggtgct
     8221 gccagtgcgc tcggcgactt tcatactggt gatatccacg caaaactcac acagacacat
     8281 ttgcgcaatg cgcgcgctga caagcgatcg cactactgga tccagcggtg agcgtttgcg
     8341 ctccagccag ccgacaaaca tcgacaccag ataaaagata aacgggatcc gcccccacca
     8401 gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc agccacgcct gcggacgtaa
     8461 aatgaaagga attttgctaa gcggcggtaa acccatcagg cattcctctt ctggcgctca
     8521 agccatattt tcttcgccat aaaaatcacc acggtcaggg caaacagaat aaacagtccg
     8581 gtgacaaacc agaaagtacc gcctgttaat atgctgcccg cccaggaata aacaatagtc
     8641 gctggtaact gaccaagccc ggtggcgata aaaaacgagc gaaaacgtat tgaagtcaaa
     8701 ccggcagcat agcttacggg atcaaaaggc acaaaaggca ataatcggca taccagaatg
     8761 gtgtgtttac catagcgggt aaaaaagccg tccatactat tgagtaccgt tttacccgtt
     8821 aacttttcca ccacttcgcg gcccatcact ctggcgataa aaaagcacag cgccgcgccg
     8881 gccatcgaac tggtccacga cagcaagccg ccccagagcg cgccaaacag cgacgcattg
     8941 gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg cctgtaaaat catcaacaag
     9001 aacgagacaa ctgccgccag tgcgccgtaa gactgaataa agcgttctat accctgttgg
     9061 tccacggcgg caaacgccgc aacgctgcga ttaataaact catggacgcc gggaagcagc
     9121 gcccatgcca gcagcatagc gaacaacagg atgatgaggg ttatacggta gtaccagatt
     9181 tttcgcgatt gcatcttcat catcctttat cctggcttta ttcggtgatt ttaaaggtaa
     9241 ccgttgccag cgtgttatcc gccgggagaa ccgaggcatt gcctttgaat ttcacttcgc
     9301 cgcgtttttc caccgcacca taagtgtatg ttgcattgct gacgatgccg accgggcagc
     9361 tatccagaca caccaggcag cccgttttct tctcttctgc tgccgttaaa ttaccgccaa
     9421 agcgcatgtc cagtttcttg ccattactgt caacaatcac ttcatcgaag gaatacgctt
     9481 ttgccgcccc ttgccagttg accgaaatat ccagtttgct gcctgcgacg tgagtcgttt
     9541 ctttattatc catcgtcatg ttttcgcccg gtgtaccacc tgcctctttc agggcttcat
     9601 aaaatgcttt cggggtcgca taacccataa acagcgattt atgcccgttt gagccatctt
     9661 taaacacaat gccgtggcgg gtgtcgtcgg tcaggaaacg accattaatt tgcaccagca
     9721 tggttaccgt atggttattg gcatcgacac gcagcggttg gctgttcgaa agtccgtcgt
     9781 attccacttt tgcagccgca ttctcttttt gatcgcagcc gctaagcccg gcaacagcca
     9841 gtaagcagag tgcagccagt ccttttttcc atgacgctga ataatgttgc aacatcgttc
     9901 tatccttcat tttttgggtg agtaagtggg ctgcggcgcg aagcagacag atccacatgt
     9961 ttgtgtcggg cgtagagttt ggcgagctgg acgagaataa acagcgccag gcccgccaga
    10021 cagagttgta aaataaagcg cagcgtaatg ccttcattgg cgagatcgct tgccatcacg
    10081 gtataaataa caataccggg cagggtcgtg agtgccgaaa taagggtata aggccagaag
    10141 gcgatggtgg ttaatccgta agcgtaattt tgaatattgt aaggaaacaa cgggattaag
    10201 cgggtcagaa taagaaaatc aataccgtta cgcgcaatgc ctttttcaat ggcctggaag
    10261 gtatggctat ggccaacgta tttcagcagt aaatcacgtc ccagccagcg cgccagcagg
    10321 aatgagcacg aggaggccag cgtggcggca attaatgata gtagtgtccc taagagcggg
    10381 ccaaaaacta ttcctccagc gatcaccagt atgcttcctg gtaacagaaa gagggtggca
    10441 atgatgaata acagaatata gaggctataa ccgaacaatc cgctctggcg aatgagtgtc
    10501 tgtaagtggg gtaaatcggt gagcagatcg aataaaccga aagcgtggat agcgtaaatg
    10561 agcagcgcaa aaataagaca ggcaaaaaga aatttacgct cagcgttcat tgtggagttt
    10621 cctatatcaa acttgataat agagaagtgg tcagacaact aaaaacgcag tgacggtata
    10681 acaaagggcg gcaggcaaca aatcattact gacagtttgt tgatttttct caaggttaat
    10741 tctcctgacc cagtttgagc caggagaaca gctaaattag cggcggaagg tcgcccagac
    10801 gggggcatga tctgacggtt tttccatgct gcggatttca tagtcgatgc cggtttcaac
    10861 acaacattct gccagcggtt ggctggcgag aagcaggtcg atgcgcagac cacggttatc
    10921 gtcaaaacct tttgagcggt aatcaaacca tgagaaacga tcggctgttt gcggattcgc
    10981 atggcggaag gtatcgacca acccccagct catcagtctc tccatccatt cgcgttcttc
    11041 cggaaggaaa gagcattttc cggtacgcag ccagcgctta cggttctctt cgccaatgcc
    11101 aatatccagg tcgccaggac tgatattcat atcacccata atcagcaccg gattttcacg
    11161 cttgagttcg gtttccaggt agttttgcag attctgataa aactgcgcct ttgccgggaa
    11221 ttttatcgga tggtcgcggc tttcaccctg cgggaagtaa ccgttgatca cggtaacatt
    11281 gcccagcgtc gaggggattt cagccataat aattcgccgt tgcgcctctt cgtcatcacc
    11341 cggaaaaccg cgacgcacgg caatcggcgt ctctttggtc agcagcgcca cgccataatg
    11401 gcctttctgc ccgtgataaa acacgttgta gccgagcttc gccacctctt cgagtggaaa
    11461 catatcgtca tgaacttttg tctcctgcag gccaatcaca tccggttggt gcttttcgac
    11521 gatggcttca agctgatgag gtctggcgcg caggccgttg atattaaaag agacaaattt
    11581 catagtcgct gccatttatt cagataaaga gtgcgtggat ggtagcagaa tttcgctttg
    11641 cgtaccgcct gttgttaagt gatttgatcg atagttgtta atgatgtcaa cgatggcgca
    11701 aaaaat
//
LOCUS       DARNVM010000153        11688 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.153, whole genome shotgun sequence.
ACCESSION   DARNVM010000153 DARNVM010000000
VERSION     DARNVM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.153"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            3..566
                     /locus_tag="SI621_003773"
     CDS             3..566
                     /locus_tag="SI621_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HEI4066434.1"
                     /translation="MVFTCIGAALFGQISAASNCWSNHVGIIIGHNGEDFLVAESRVP
                     LSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGFKYESSR
                     QFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWERKTVTPA
                     SLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(568..1089)
                     /gene="ruvC"
                     /locus_tag="SI621_003774"
     CDS             complement(568..1089)
                     /gene="ruvC"
                     /locus_tag="SI621_003774"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEI4066435.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFEPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1124..1864)
                     /locus_tag="SI621_003775"
     CDS             complement(1124..1864)
                     /locus_tag="SI621_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEI4066436.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1893..2345)
                     /gene="nudB"
                     /locus_tag="SI621_003776"
     CDS             complement(1893..2345)
                     /gene="nudB"
                     /locus_tag="SI621_003776"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEI4066437.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2463..4235)
                     /gene="aspS"
                     /locus_tag="SI621_003777"
     CDS             complement(2463..4235)
                     /gene="aspS"
                     /locus_tag="SI621_003777"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEI4066438.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANNPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4545..5111
                     /locus_tag="SI621_003778"
     CDS             4545..5111
                     /locus_tag="SI621_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEI4066439.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAVKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5108..5926
                     /locus_tag="SI621_003779"
     CDS             5108..5926
                     /locus_tag="SI621_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEI4066440.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKS
                     EVVLRWREQTTDNFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5979..6374
                     /locus_tag="SI621_003780"
     CDS             5979..6374
                     /locus_tag="SI621_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEI4066441.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6415..7158
                     /gene="cmoA"
                     /locus_tag="SI621_003781"
     CDS             6415..7158
                     /gene="cmoA"
                     /locus_tag="SI621_003781"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEI4066442.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7155..8126
                     /gene="cmoB"
                     /locus_tag="SI621_003782"
     CDS             7155..8126
                     /gene="cmoB"
                     /locus_tag="SI621_003782"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HEI4066443.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8161..10590)
                     /gene="torZ"
                     /locus_tag="SI621_003783"
     CDS             complement(8161..10590)
                     /gene="torZ"
                     /locus_tag="SI621_003783"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HEI4066444.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAVDQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLAGKSDNTPKSAAWAAEISGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGRDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNRRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10615..11688)
                     /locus_tag="SI621_003784"
     CDS             complement(10615..11688)
                     /locus_tag="SI621_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEI4066445.1"
                     /translation="MFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQ
                     GTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFEAHRQ
                     EMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIA
                     HFPPEIKMDDNAAHELESQAATSVTNGVHIYPFKTSRIGELATVNPGTDLIVVDASGK
                     QPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQAS
                     LQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIAKGMGARTSM
                     SENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2649 a         3119 c         2870 g         3050 t
ORIGIN      
        1 atatcgtctt tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt
       61 gctggagtaa tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag
      121 aaagccgggt tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta
      181 atcaacgcta tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa
      241 ttgttgaaca ggttccctcc cggctacgca aactttacca caccggtttt aaatacgaat
      301 cttcgcgcca gttctgttca aaatttgttt ttgatattta taaagaggcg ctatgtattc
      361 cggtgggtga aatagagacg tttggagaat tgttaaatag caatccgaat gcaaaactca
      421 ctttctggaa attctggttc ctgggttcta ttccgtggga gcgtaaaacc gtcacgccag
      481 ccagtttgtg gcatcatccg ggtttggtgt tgattcatgc ggagggagtg gaaacgcctc
      541 agccggaact gaccgaggcg gtataactta acgcagtcgc cctctcgcca ggttcagccg
      601 cgattcgctc atctgcatcg cattctgact gacgtgacaa tgggtaatcg cgatagctaa
      661 tgcatcggca gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg
      721 ctgcacctgg cttttttcag cactaccaat acctaccacc gtttgcttta cctgacgtgc
      781 cgcatattca aataccggca attcctgatt caccgccgcc acaatcgcca cgccgcgcgc
      841 ctgacccagt ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa
      901 ataatcaggc tcgaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg
      961 agacggtaaa tcatccactt tggtgcgtat gcatccgcta cccaggtagg agagttgcct
     1021 gcctacctgg cggatgacgc catagccggt cacgcgcgaa cccgggtcaa tgccgagaat
     1081 aatagccatc acgcgtctcc gttttgctgt ttagcaggcc ccatcagaga gtcgctgcaa
     1141 cctcatcgga gatttcaccg ttatggtaga cttcctgcac gtcgtcgcaa tcttccagca
     1201 tatcgatcag acgcatcagt ttcggtgcgg tttcagcatc catatcagct ttggtggacg
     1261 ggatcatgga aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgta
     1321 ctttacccat ttcttcccat gcagtgtaga cgtcaatcgc gccgtcatca taggtcacaa
     1381 cgtcttcagc acccgcttcc agagctgctt ccatgatggt gtcttcatcg cctttctcga
     1441 aggagatcac gccttttttg ctgaacagat aggctacgga accatcagta ccgaggttac
     1501 caccacattt gctgaatgca tgacgcactt cagcaacggt acggttgcgg ttgtcagaca
     1561 gacattcaat catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca
     1621 tgtttgcatc atcatcaccg cccacaccac gtgcaattgc gcggttcaga gtgtcacggg
     1681 tcatgttgtt agacaatgct ttatcaattg ctgcacgcag acgcgggtta gcgtccgggt
     1741 caccaccgcc cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac
     1801 cgcgcttagc atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg
     1861 ccataaaaat atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca
     1921 atcgcctgcc ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac
     1981 ttgtaggcca gatgttcagt gaagacgacc tggcgttcgt gcgggagcgc aagacagaac
     2041 catgattccg tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt
     2101 tcaaattcta ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcgatg
     2161 gtgacctctt cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg
     2221 ctgccggtta ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt
     2281 ttcgtatctt gcgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc
     2341 ttcacgctgc ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc
     2401 atgggagttc actcagttgc cgcctcgatg caacgcgaat gatttcgtgt atttgagtca
     2461 tatcagttat tctctgcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg
     2521 ttagcaaagc tcggtgcttc agtcatcaga cacgccgccg ccgtggtttt cgggaaggcg
     2581 ataacgtcac ggatattgtc ggtgccggtc agcagcatcg tcagacggtc aagaccgaat
     2641 gccagacctg cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc
     2701 tcgcgctgtt cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca
     2761 ttatggatac gcacagaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg
     2821 ttcgccaccg cattttctgg tgcagctttc agttctgcag cagtcatgtc tttcggtgag
     2881 gtgaacggat ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaaa
     2941 tcgataaccc acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact
     3001 ttcaggcgca gtgcgcccat cgcgtcggca acaattttct tgttgtcggc accgaagaaa
     3061 atcatatcgc catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta
     3121 aggaacttcg ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg
     3181 atgtaagcca gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc
     3241 ttacgggtca gcgatgcgcc acccggaaca cgcagagcgg caacgcgccc tttcggattg
     3301 ttcgccggac ctgcaaatac tgcaaattca acagatttca gcagatcggc aacgtcagtc
     3361 agttccatcg ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca
     3421 aaggtcatta ccgggaaatc gcccagatcc acgcccttca cttccagcca cagatgacgc
     3481 accagcgctt ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg
     3541 atctgagtaa attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg
     3601 atctgatagt aacggtcaaa gccggacatc atcagcagct gtttgaacaa ctgtggggat
     3661 tgcggcagtg cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg
     3721 ccttccggcg tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc
     3781 ataaaacggc gcaccaggct ggtgatttta gcgcgggttt tcaggcgctg agccatttcc
     3841 gggcgacgca ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta
     3901 gagtcaagcg gcagaacatc tgcacggttg atgatagtca gcgaggacgc cagtacttcg
     3961 atttcgccag tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc
     4021 gtgacctgaa tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga
     4081 tccggatcga aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg
     4141 ctaccaagat cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg
     4201 gacaaacgga gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg
     4261 gcggatgccc cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga
     4321 atgaaaaaag gcggctatta tactggaaat tctgccgcac cgtaagagtc tggcccgcgc
     4381 tggaacgcct cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta
     4441 taaaggtgtt gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat
     4501 cgcattaagg tgagaggcac gattttgttt tgtcaggagt catcatgctt gaacttaatg
     4561 ctaaaaccac cgcgctggtg gtgattgatt tacaagaagg catcttacct tttgccggag
     4621 gtccacatac tgccgatgag gtggttaatc gcgccgggaa gctggcggtg aaatttcgcg
     4681 ccagcggtca gcccgtgttt ctggtgcgcg ttggctggtc tgccgattac gccgaagcat
     4741 taaaacagcc ggttgatgcc ccttcccccg ctaaagtgtt gcccgaaaac tggtggcaac
     4801 atcctgctgc attaggtgca actgacagtg atatcgaaat catcaaacgt cagtgggggg
     4861 cgttttacgg tacagatctg gagttgcaat tacgccgccg gggtatcgat acaatagtat
     4921 tatgtgggat ctcgaccaat atcggtgttg aatccaccgc ccgcaatgcc tgggaactcg
     4981 gttttaatct ggtgattgcc gaagacgcct gcagcgccgc cagcgccgag cagcacaata
     5041 acagcatcaa ccatatctac ccacgcatcg cccgtgtgcg cagtgtggaa gagatcctca
     5101 acgcattatg atttacatcg gtctgccgca atggtcgcat cctaaatggg tgcggttggg
     5161 gatcaccagc cttgaagagt atgcccgcca ctttaactgt gtggagggca acaccacgct
     5221 ttacgccctg ccgaaatccg aggttgtcct gcgctggcgt gagcagacca cagataattt
     5281 tcgcttctgt tttaagtttc cggcgaccat ttcgcatcag gcagcattac ggcattgcga
     5341 tgatttagtg actgaatttt tgacccgcat gtcaccgtta gcgccgcgca ttgggcaata
     5401 ctggctgcaa ctgcctgcca catttggccc acgggatctg cctgcgcttt ggcattttct
     5461 cgattccctt cccggcgaat ttaattatgg tgtggaagtc cgccatccac agtttttcgc
     5521 caaaggggaa gaggaacaaa cgcttaatcg cggtttacat cagcgcggcg ttaatcgggt
     5581 gattttagac agccgcccgg ttcatgcagc acgtccacac agtgaagcta ttcgcgacgc
     5641 tcaacgaaaa aaacctaaag ttccggtaca tgctgtactg acggcgacaa atccgctgat
     5701 ccgttttatc ggtagtgatg atatgacgca aaaccgggaa ttatttcagg tctggttaca
     5761 aaaattagcg cagtggcatc agaccactac gccttatctt tttttacata cgccagatat
     5821 tgcccaggcc ccggaactgg tacataccct gtgggaagac ttacgtaaaa cgcttccaga
     5881 gatcggagca gttccggcta ttccacagca atcttctctt ttctgaattt gccacctatc
     5941 atagacaggt gccatcggcc attttaaagg gagtttgtat ggtaagcgcg ctgtatgccg
     6001 ttttaagtgc gttgttatta atgaagttct cttttgatgt cgttcgcctg cgaatgcagt
     6061 accgcgttgc ctatggcgac ggcggtttta gcgaactgca aagcgctatt cgcattcatg
     6121 gtaacgcggt ggaatatatt cccatcgcga ttgtgttaat gctgtttatg gaaatgaatg
     6181 gcgcagaaac ctggatggtg catatttgcg gcatcgtttt gcttgctggt cgtctgatgc
     6241 attattacgg ttttcatcac cgtctgttcc gctggcgacg ttccggcatg agcgccacct
     6301 ggtgtgcgct gttgctgatg gtgctggcga atctttggta tatgccctgg gagttggttt
     6361 tctccctgcg ttagcgcaca atacgccact ttctttttcc cggattttta cgttatgtct
     6421 caccgcgaca cgctattttc tgcccctatc gccagactgg gcgactggac ctttgatgaa
     6481 cgggtagctg aagtcttccc ggatatgatc cagcgttccg ttcccggcta ttccaatatt
     6541 atttccatga ttggtatgtt agccgagcgc ttcgttcaac ctggtacgca ggtttacgat
     6601 ctgggttgtt ctctgggcgc ggcgacgctc tcggtgcgtc gcaacattca tcatgataat
     6661 tgcaaaatta ttgccatcga caactctccg gcgatgattg aacgctgccg tcgtcatatt
     6721 gacgcctata aagcccctac gccagtagac gttattgaag gtgatattcg cgatatcgcc
     6781 attgaaaacg catcgatggt ggtgctgaat tttaccctgc aattcctgga accttccgag
     6841 cgtcaggcgt tactggataa aatttatcaa gggctgaacc cgggcggtgc gctggtgctt
     6901 tcggaaaaat tcagtttcga agatgccaaa gttggtgaat tgctgtttaa catgcaccac
     6961 gactttaaac gtgccaacgg ttacagcgaa ctggagatca gccagaagcg cagcatgctg
     7021 gaaaacgtga tgctaaccga ttctgtggaa acccataaag cacgcctgca tcaagctggt
     7081 tttgagcata gcgagctgtg gttccagtgc tttaactttg gttccctggt ggcattaaaa
     7141 gcagaggacg ctgcatgatc gactttggta acttttattc tctgattgcc aaaaatcatc
     7201 tttcacactg gctggaaacg ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc
     7261 acgggctgtt taagcagtgg tccaacgcgg tggaatttct gcctgaaatt aaaccgtatc
     7321 gtctggattt attgcatagc gtgacggcag aaagcgaaga gccactgagc gccgggcaaa
     7381 ttaaacgtat tgaaacgctg atgcgcaacc tgatgccgtg gcgcaaaggg ccgttctcac
     7441 tgtatggcgt caacatcgat accgaatggc gttccgactg gaaatgggat cgcgttctgc
     7501 cccatctttc tgatttaacc gggcgcacca ttcttgatgt cggctgtggc agcggttatc
     7561 acatgtggcg catgattggc gcaggcgcgc acctcgcggt gggaatcgac cctacgcaac
     7621 tgtttctgtg ccagtttgaa gcggtgcgta aactactggg taacgatcag cgcgcacatt
     7681 tgttaccgtt aggcattgaa cagcttccgg cactgaaagc ctttgatacc gtcttttcga
     7741 tgggcgtgct ttatcatcgt cgttcaccgc tggagcatct ctggcagttg aaagatcaac
     7801 tggtcaatga aggtgaactg gtgcttgaaa cgctggttat tgatggtgat gagaacacgg
     7861 tgctggtgcc gggcgatcgt tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc
     7921 tggcgctgaa aaactggctg aagaagtgtg gttttgttga tattcgcatt gcggatgtga
     7981 gcgttaccac tacagaagag cagcgacgca ctgaatggat ggttaccgag tctctgtccg
     8041 atttcctcga cccacatgat ccgagtaaga cagttgaagg ttatccggca cctaaacgcg
     8101 cggtgctgat tgcgcgcaag ccgtaaagat tagcgcccgt ttccgggcgc ttttcacgtc
     8161 ttatgcctga acagctggct gatcaaacgc cgttaacttc ggtgcattgc ccgtatattt
     8221 ttcgatatac accagcgcag agttaccggc acaggcattt gccagctgag agctggggat
     8281 atccgccgtt aacacgttcg cactgccgtt tttacacaag ccattttcca gatctggcca
     8341 tgcaccttca tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt
     8401 cagaatctga ccacgtctgt tccagacacg caccagatcg ccattcgcaa taccaaagcg
     8461 agcagcatct tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg
     8521 cagttccgca tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg
     8581 caactgcttc tcgtcggcgg aacctttcca ctcatcaggc gcaagccagg ttgggtgtgc
     8641 cgggcaatcc ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct
     8701 tggcgtaccc agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg
     8761 ttcgttcttc tcgctgcggc gcatttcgat cagtttattt tgctgccaga aagcattaaa
     8821 catcggcata gtcacgcgtt gcgcacgcgc acctttctga gcggcatcat agaaaaattt
     8881 cagccacgcc atttcatctc taccttcggt atagatctct tttccgccag gtttgagtaa
     8941 ttcagcaaga tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg cagcgacagc
     9001 ctgcttcatc ggtacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg
     9061 ctcaaacgat gtggtgatcg gtaatacgat atctgcatgt ttagcagctg cggtccagta
     9121 gcattcagaa acgacgatca tctccggttt ctgccatgct ttaatcagac gattagtatc
     9181 ctggtgatgg gtaaagttac cgccacccgc ccaccagatc attttgatat ttgggtacgt
     9241 ttgctcttta ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg
     9301 ggcaacagga atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat
     9361 cgccgcagac atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta
     9421 atgataagag aaaccaaacc cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag
     9481 cgtcaccagc atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc
     9541 catcagtatt gtgcgatttg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg
     9601 tacgccagaa atttccgccg cccaggccgc gctcttcggc gtattatcgc tcttgcctgc
     9661 cagatactct tcaaactgcg gatatccggt agtgtatttt tcaaggaaca ctttatcgtg
     9721 tttgccttgt gtcatcaggg tatgcgcgat ccctaacatc agtgccacgt cggtgcccat
     9781 attcggcgcg atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcgaatagg
     9841 atcaatggca atcacgggtt tgccagattt tttcagctga tggaagtatt ccagcccttg
     9901 ctcatcggta ctgctccagg caattttcaa ggtattcagc gggttcatcc cccacagcac
     9961 cacaacctgg ctgttttcca gaatcagcgg ccaggaggtc tgctgttcat acacctctac
    10021 agaaccgacc acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg
    10081 cccggaataa ccgcccgcaa ggttcatata acgttgcagt aaggtttgcg ctttatgcaa
    10141 cacgccagaa gatcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt
    10201 acgaatacga tcgtgttgct catgaatcag ttttaatgct tgttcccagc tcacctgtac
    10261 ataggtatct tcgccacgac ctttcgccgg ttgcagtgga ttatcgagat agctttttct
    10321 caccatcgga tgctgaatac gcgccgtggt gtgtacctga tccaccgccg tagactgtaa
    10381 ggagttcggt atagttttcg ccagcgcgcc tgttgaagaa acaatcttgc cgtccttcac
    10441 ttctacgttc atcgctcccc aacggcccgc ggtgaggatt ttgccaccct tctcttctgc
    10501 ccatgcgggt agcggtgcag ccgatgtcac caccagcgcc ccagcggcaa taccgctgtg
    10561 tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg aggatcactg
    10621 tttctcggta atatctttgg cgttgtactg gaagtaccgc gtcaaaatgt ccagttcgtt
    10681 ttcgctcatg ctggttcgcg cccccattcc tttggcaatg gacggccatg cattgacggt
    10741 gtaatggtcg gaggcaatag gggcatggca accagcgcaa taggtatcgt caagtttttc
    10801 agcgtattgc catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa
    10861 agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag
    10921 cgccttgata ccttcttcac ttaatgtggc tagtgccagc cgttgacctg ccgccaggta
    10981 gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc
    11041 actggcatca acgacgatga gatcggtacc aggattcacg gtagccagct cgcctatgcg
    11101 agaagttttg aaaggataaa tatgtacgcc attagtcact gaagtagcgg cctgactttc
    11161 cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatgag caatgccttt
    11221 atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga
    11281 ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga
    11341 gtcagtggct tttaattctt tccatactgt ttcggccatt tcctgacgat gcgcttcgaa
    11401 cttatcgtca ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt
    11461 taatttagca aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc
    11521 acgtatccct ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact
    11581 catcgagtgg caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc
    11641 cagcaataac ccgccgccac caaccacaac cgctatcagc aaaaacat
//
LOCUS       DARNVM010000154        11590 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.154, whole genome shotgun sequence.
ACCESSION   DARNVM010000154 DARNVM010000000
VERSION     DARNVM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.154"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            51..350
                     /locus_tag="SI621_003785"
                     /pseudo
     CDS             51..350
                     /locus_tag="SI621_003785"
                     /inference="COORDINATES: protein motif:HMM:NF037007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(252..866)
                     /gene="tnpA"
                     /locus_tag="SI621_003786"
                     /pseudo
     CDS             complement(252..866)
                     /gene="tnpA"
                     /locus_tag="SI621_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            874..2082
                     /locus_tag="SI621_003787"
     CDS             874..2082
                     /locus_tag="SI621_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEI4066446.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSASMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKCKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRTILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEIIQATA"
     gene            2126..2233
                     /locus_tag="SI621_003788"
                     /pseudo
     CDS             2126..2233
                     /locus_tag="SI621_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2217..2855)
                     /gene="ytfB"
                     /locus_tag="SI621_003789"
     CDS             complement(2217..2855)
                     /gene="ytfB"
                     /locus_tag="SI621_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HEI4066447.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNALVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3073..3693
                     /gene="fklB"
                     /locus_tag="SI621_003790"
     CDS             3073..3693
                     /gene="fklB"
                     /locus_tag="SI621_003790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEI4066448.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4002..5414
                     /gene="cycA"
                     /locus_tag="SI621_003791"
                     /gene_synonym="dagA"
     CDS             4002..5414
                     /gene="cycA"
                     /locus_tag="SI621_003791"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HEI4066449.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPELSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5459..6121)
                     /gene="ytfE"
                     /locus_tag="SI621_003792"
     CDS             complement(5459..6121)
                     /gene="ytfE"
                     /locus_tag="SI621_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HEI4066450.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQASKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6229..7194)
                     /locus_tag="SI621_003793"
     CDS             complement(6229..7194)
                     /locus_tag="SI621_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEI4066451.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFVSLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLIEPVSES"
     gene            complement(7303..8163)
                     /gene="qorB"
                     /locus_tag="SI621_003794"
     CDS             complement(7303..8163)
                     /gene="qorB"
                     /locus_tag="SI621_003794"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEI4066452.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQSLTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8252..8632
                     /locus_tag="SI621_003795"
     CDS             8252..8632
                     /locus_tag="SI621_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEI4066453.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8750..10693)
                     /gene="cpdB"
                     /locus_tag="SI621_003796"
     CDS             complement(8750..10693)
                     /gene="cpdB"
                     /locus_tag="SI621_003796"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HEI4066454.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWSDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRVGKIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10883..>11590
                     /gene="cysQ"
                     /locus_tag="SI621_003797"
     CDS             10883..>11590
                     /gene="cysQ"
                     /locus_tag="SI621_003797"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEI4066455.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESF"
BASE COUNT         2751 a         2920 c         3023 g         2896 t
ORIGIN      
        1 tacaccatac attcttgttg tgcggtatgt aattattcta cgctgtcggt atcggtgcgt
       61 tcgttaatca gcaggttaac cagccacaaa aatccattga aaaaatcatc gattttacct
      121 ggggttatta tattgctaat ccctggtttt taaaaattgt gcatagtgaa aaccaaagta
      181 aaggtgtgca ttatgcaaaa tcacagcgtc tcttagaaat taatcatgcg cacttgcagt
      241 taatggaatc cttattggat gaagggaaga aacacaatat ttttaagcca gatatcgacc
      301 cattgcaggt gtatattaat attgctgcgc ttgggggata ttatttgatc aaccagcata
      361 cgcttggcct ggtttatccg gtgcgccgta aaaccccgtc cttcagggcg gggatataag
      421 gcgcggtttt ccacctgacc aggtgtttgc tgttgttcaa tgtattggcg gatgacggat
      481 attggcgcac ctccgcagct actggcaaaa tagccaggac tccacaaaac gcctttgtag
      541 taatacctga ctgcaatatc tggtcgatct cgtcgcagtc acctacccaa tacgcctttg
      601 aggctgttta ccagactgga tatcgccagt ttgggaggat agttaattaa caaatggacg
      661 tgatctggtt cgccatccat ttcaaccagt tcagcttcaa aatcagcaca tacctttgaa
      721 aagtaagtgc gtagtttttc tttcgcgtca tggtcaaaaa tctggcgtcg gtatctggtg
      781 acaaagacca ggtgaacatg catcaggaaa acacaatgct ttccacgccg gatatcggtt
      841 tcttttttca tagaccaaag tatcatttga cctgtgaaac gattacaggc atttaaattc
      901 cagttaagac caggtggtca acaggagtgt gaaatgaggc gcttcgcagg cgcatgtcgt
      961 ttcgttttca atcgtgctct ggcgcttcag aatgagaatc atgaggccgg taataaatac
     1021 atcccttacg ggaaaatggc ttcctggctg gttgagtgga aaaatgccac tgaaacgcaa
     1081 tggcttaaag atgccccgtc acagccattg caacagtcac tgaaagacct tgagcgggct
     1141 tacaaaaact tcttccagaa gcgggctgct tttccccgat tcaaaaagcg gggacagaat
     1201 gatgcattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg tatttttctg
     1261 ccgaaacttg gctggatgcg ctaccggaat agccgtcagg tcacgggtgt tgtgaaaaat
     1321 gtcacggtca gccagtcctg cggtaagtgg tacatcagta ttcagactga aaatgaagta
     1381 tcaactccgg ttcacccttc agcatcaatg gtcgggctgg atgctggcgt ggctaaactc
     1441 gccacgctgt cagatggcac agtctttggg cctgtaaaca gttttcagaa aaaccagaag
     1501 acgctggcga gacttcagcg gcagttaagc cgcaaggtca aattcagcaa caactggcag
     1561 aagcagaaat gcaaaataca gcgactgcat tcccgtatcg caaatatccg cagggactac
     1621 cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg
     1681 aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc
     1741 cgggcaaaat caggtttaaa ccgtacgata ctggatcagg gctggtatga aatgcgccgc
     1801 cagcttgagt acaagcagct ctggagtggc ggtcaggtgc ttgctgttcc gccagcgtac
     1861 acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaatcgcct gtcacaaagt
     1921 aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaacgg cgctcgtaac
     1981 attttagcgg cgggacacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgctcg
     2041 ttgaagcagg aacccaccga aataattcag gcgacagcct gaacgtagca gggaaccacg
     2101 tccttcaggg cgtggaggat gtcaacacat cagtatggtt tcaccccagg cactggaagc
     2161 cagacgtaag gtcatcaaag agacaatcct tagctggctt ttggttgacc cttcatctac
     2221 cgcgcacgaa taaaactgcc atccggctgg cgggtaaaca agacctgttg attattcccc
     2281 gtatcaatgg ttaagcccgt caccacgccg ctggcgttct ggcggatttt caccatctgc
     2341 ccgttttgca aattactcag cggcttaccc gcgccttcaa cctgcgccat tgcatagaca
     2401 tcggtagcgg gcagtccgtg gtcgcggaac agctgcgcca tcgttttacc gggttcgaca
     2461 cggtaagagc gccattgatt atcaattccg ctatctggct ggaatggttg cgtttgggtg
     2521 gtttgcggct gctcttctgg ctggccttcc tgtattggtt ctggagcgac cggggccacc
     2581 tgatcaggat cattttgtgg cgtaactagc tgcgcccgca gctgttcttc tgtcggtggt
     2641 tgtgattgcg actgaatatc taactgcgct tcacgcgtca ccaccagcgc gttgggcgta
     2701 tcatcagatg gaagcagaaa tccgaccacc aatacaatgg cggcaataat gatcccccga
     2761 cgatgcatcg gcggcagcgg gtccatgaag cgaaaattgt ctggcgcgtg ccagactttc
     2821 tccagggttg gttttagttc aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt
     2881 tcctcaatca tagcctatga ataagctaac gctatgatgt ccgtggtaaa cccgccttta
     2941 ttatattcat acgggatatt gctattgttt ctttttcccc gggatttgtc atcattcccg
     3001 cgacaaagtt ttacccaaag aagtgtggct gatatgctgc ccgctacttt ataccctaag
     3061 aaaggaaata cgatgaccac cccaactttt gacaccatcg aagcgcaagc aagctacggc
     3121 attggtttgc aggtagggca acaactgagt gaatctggcc tggaagggct gctgccagaa
     3181 gcactggttg caggcattgc tgatgcgctg gaaggcaaac atccggctgt tccggttgat
     3241 gtggtgcacc gcgcgctgcg tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt
     3301 ttccaggcga tggctgctga aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt
     3361 gtaaatagca ccgaatctgg cctgcaattc cgcgtgatca accagggcga aggcgcaatt
     3421 ccggcacgta ccgaccgcgt tcgtgttcat tacaccggaa aactgatcga cggcaccgtg
     3481 tttgacagct ccgttgctcg cggcgagcca gctgaattcc cggttaacgg tgtgatccct
     3541 ggctggattg aagcactgac tctgatgccg gtaggttcta aatgggaact gactatcccg
     3601 caggaactgg catatggcga gcgtggcgca ggcgcgtcca tcccaccgtt cagcaccctg
     3661 gtgtttgaag tcgaactgct ggaaatcctc taagcagcgc attctgttcc cctcgaacca
     3721 gaggggagca gacattcagc aataaaccct tcagtttgcc aaacggcgct attttgtgtt
     3781 gcaaagaccc cgtaagcgtg tatttttgtg agctgtttcg cgttatcacc gtgatatgac
     3841 actcacttta aacataaaat taacattaga tctaaatctt agtattcatc ccgcgtattg
     3901 ttacctaata tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg
     3961 tagagcctga acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag
     4021 tcgttgccga tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac
     4081 atattcagct tattgccatt ggcggtgcca ttggtacggg gttgtttatg gggtccggca
     4141 aaacgattag ccttgccggg ccgtcgatca ttttcgttta tatgatcatt ggttttatgc
     4201 tctttttcgt gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt
     4261 ttagtgactt cgcttccgat ttactcgggc cgtgggcagg atatttcacc ggctggacct
     4321 actggttctg ctgggttgta accggtatgg cagacgtggt ggcgatcacg gcttatgctc
     4381 agttctggtt ccccgagctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc
     4441 tgacgctcaa cctcgccacc gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga
     4501 tcaaaatcgt cgccatcgtg tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact
     4561 ttcagtcacc gaccggtgtg gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt
     4621 tcccgaaagg tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtgg
     4681 ggattgagtt ggtaggtaca acagctgcgg aaaccaaaga tccagagaaa tcactgccac
     4741 gcgcgattaa ctccattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtga
     4801 ttatgtccgt gacgccgtgg agttcggtag tcccggagaa aagcccgttc gttgaactgt
     4861 tcgtgttggt aggtttgcct gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg
     4921 cggcgtcttc cgctaacagc ggtgtcttct ctaccagccg tatgctgttt ggtctggcgc
     4981 aggaaggtgt ggcaccgaaa gcgttcgcta aactctctaa gcgcgcagta cccgcgaaag
     5041 ggctgacctt ctcttgtatc tgtctgctcg gcggcgtggt gatgttgtat gtgaatccca
     5101 gcgtgattgg cgcgttcacg atgattacaa ccgtttccgc gattctgttt atgttcgtct
     5161 ggacgattat cctttgctcg tacctggtgt atcgcaaaca gcgtccgcac ctgcatgaga
     5221 aatcaatcta caagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcattctttg
     5281 tgttcgtggt cgtgctgctg acactggaag atgatacccg ccaggcgctg ctggttaccc
     5341 cgctgtggtt tatcgctctg gggctgggct ggttgtttat tggtaagaag cgggctgctg
     5401 aactgcggaa ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatc
     5461 actcacccgc cagggcgcgt gggaacagta cattgttttc cagactgatg tgatccatca
     5521 ggtcatcaat cagttcatta ataccgttat acatcgcttt ccaggtggtg caggcttctg
     5581 gcggcggtgt gacgttattg gtggtgtgtt taatcacttc cagcagttcg cccgcttcat
     5641 cgtgctcgct ttccatcacg ctgattggtc ccattgcctg gctgcccatg ccttgtttga
     5701 tcatcgggaa gagaatttgc tcttctttca tcatgtggct ggaaagctct tcatgcagca
     5761 tagtcaggta ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgttcga
     5821 ctttgctggc ttgcagaatc agctccggca gttgctcgcg gtgacgatcg tggtagcgca
     5881 cgatgatatg gtcgatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt
     5941 gttcagcgag ctttgccagc tcagcttcaa tgacctcaac atccagctct ttacgtgccg
     6001 ccgcacgcgc cagcgtttgc ttaccgccgc agcagtaatc catatcatat ttacgaaaca
     6061 gagccgaagc gcgaggaata gagagcgcca gttcacctaa aggttggtcg cgataagcca
     6121 tagctgatac ctcattctta ataagataag atgcatttta aatgcatctt taaggcaaaa
     6181 gctataaccc ttacgtagca gaggcttatt taactcactg ccagcgtgtc aactttcaga
     6241 gacaggttca attaagggtt tttccggttt tgctctgacc gcaatgacca cgccaaccac
     6301 taacagcgcg ataccgctca gcgtcattag tggcggcatt tgctggcgta acaaaaaggt
     6361 gtacagcaaa cctgccagcg tttcgaagac aatcagtggc ccgagaatca ccgttggtaa
     6421 tcgctggctg gcgacgttcc agcaaagtgc gccgacccat gagcaaagca cagctatcgc
     6481 aatcatcagg ctaacaaata caagcggacg ggggccaaaa ggtagggaga agtccggcgt
     6541 ttgcgtattc agccagtaac aagcgacgag atagccgata agcgaaaccg gcagcgtgac
     6601 cagcgcctgc gccgtcgccc acatcatcgg atgtttgtcg ggattttccc gcagccagcg
     6661 ggcgttgcgc agggcatacc atgcccagca gaccacggaa actaacgcca gcacgatgcc
     6721 agaggtataa cgtgcccagt caaaatcggg gagtccgtgg tttaactcag caatattcac
     6781 actcgccagg ccgatgccaa tacaaatcag tgccggggcg agttttcccc acgcgagttt
     6841 gccgtcgcgc tggctataaa gcagattggc aaagacagga atgaccaccg gcagggtgcc
     6901 gataatcatc gtggaaacag gcgcgccagt acgttgaatg gcactggcaa ggcagaagta
     6961 ataaatgagg ttgcccatca tagtgagcat caaggcggta agccagtcgc gacgcgccaa
     7021 ctgacgcaga cgcacgcgcc ccagccaggc aatgggcagc gcaattaacc ctaacgccag
     7081 ataacgcccc atagactgca acatcgccgg gtattccggc acgatcaacg gcccgacaaa
     7141 aataagcccc cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg
     7201 catccattgg tctgtcagaa gaaacgttca ggaagcagac atgattgctc ccggcatccc
     7261 gtcattatca gagaggatgc gggaggatgc cactttaatt aactagttat taacattaaa
     7321 aagatggctt acgctttcgg ctaacgttgt cgttgggcgg ccaatcaatt tgctaagcgt
     7381 tttgctgtta tcaaacagac cacctttcga tgcgccaacg tcagaatccg ccagcatatc
     7441 cgccagtccg tcgggcagtc cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag
     7501 attttgatag gtaacggatt ttccgctctg tttggtcagc tctgccgcta actgtgtcaa
     7561 cgtccaggca ctatcgcccg ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat
     7621 cacgcgtgcc gcagctgccg cataatctgc ccgcgttgct gaggcgattt tgccatcgcc
     7681 cgccgcaccg ataaatacgc cgtgttccag tgctgccggg gcgctggcga ggtagttttc
     7741 ggtgtaccag ccgttgcgca gcagggtgta aacgatgcca gaatcagcca acattttctc
     7801 cgtctcaatg tgctcatcgg cgaggccgag cggagattta tccgcgtgaa gcagactggt
     7861 ataagcgata aatttcacac cagccgcctt tgcggcatta ataacattac gatgctgcgg
     7921 ggcacgttga cccacttcgc tggaagagat cagcagtagt ttttccactc cctgaagtgc
     7981 agatgtcagt gcggcttcat cgccgtagtc agcctgacgc acggtaatgc cttgtgctgt
     8041 cagtgactgg gcttttgccg gattacgaac gatagccact atttggctgg caggaaccgt
     8101 tttcatcaag gattcaataa catagtgacc aagttggcca gtggcaccag taattgcgat
     8161 cataggaagt ctccatcgtt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt
     8221 tagtaagtac gtacaaaaag gtaagtatga gatgagtcag gttagcctgt cgcaacaact
     8281 gaaagaggga aatctcttcg ctgaacagtg tccgtcgcgc gaggttttga aacacgtcac
     8341 cagccgttgg ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga
     8401 cctgcggcgc aaaatgggcg gggtgagtga aaagatgctt gcgcagtcgt tacaggcgct
     8461 agagcaggat ggttttctta accgtatcgc gtatccggtg gtgccgccgc atgtggaata
     8521 tagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat
     8581 tgagttgaat ttgcccgagg tgttggcggt gcgggatgaa cgtgcggcgt aactgcagga
     8641 tgcgcggcct acggtgtatg acttgtaggg cggataagat gcgccagcat cgcatccgcc
     8701 aataaatgcc ggatgcggtt gatgatttgc aggccgaaaa agaagtgtat tacttactca
     8761 aatccacctg ataaatcgca aacccgatat catcggtcgc gactttattc atcggatatt
     8821 gccctttctc tttaataaac gccgcggctt tatctgatgg agaggtttcg aaacggatat
     8881 ccagtttctt atcgccagct attggtgcta aacgccagtt gttatctgcc gccgggtgaa
     8941 ttttccccac acgtttcgac tcatcagcaa tccacgctgc cagcaccgag cggttctcat
     9001 ccggtgaagc aaaagcgata tggctgtcgc ccgtaccggc aaatttgccg ccgtaagcgc
     9061 gatagttatt ggtggcaacg aggaacatgg cgttcggatc aatcggtttg ccattaaagg
     9121 tcaggttctt aatcctttcc gcattggcat taatcatctg gcactcgccg tcataacggg
     9181 cgggttgggt aacatcaatc tgataattca caccatcaat aacatcaaag ttataggtgc
     9241 ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct gttaggatca atctggttaa
     9301 actgtccggc ggagcactcc agccactctt tcacctcttt accgctggct ttcaccacaa
     9361 tcagcgtatt ggggtagaga taaagatcgg cggcattacg gaaggtcaac tggccttttt
     9421 ccacctccac atagcttgcc gggtcatttt tgcgaccacc aactttaaac ggtgcggcag
     9481 ctgaaagcac cggcagtttt gccagatccg gatcgccctg aatgtaatgc tcgacatacg
     9541 ctttttgcgc gttgttcact acctgcacgg tcggatcgtc ttgcactagc gccagatagc
     9601 tatacatatt gtcggcggat ttaccgattg gcttgctgac gaactggcgt gtggcatcgt
     9661 gatcggcttt gagtgtttct accagcttgc tgtcttccgc cgcgagggat tttttattcg
     9721 cgatgtcgta aatcggccgt gcttccgctt tcgcctgcgt cacctgccat ttaccgctgt
     9781 cattactaag ttgtaagtcg accacaccaa gatgatcgct ccacatgcct ggcattaccg
     9841 ccggaacacc attcagcgtg cctttcgcga tatcagcccc ttcgatatca gcaaaatctt
     9901 tacctgggaa aacggcgtga gcatggccaa acataatggc gttaacgccc ggaatttcac
     9961 tgaggtaata aactgagttt tctgccatca ctttatacgg atcggcagac agaccagaat
    10021 gcgccagaac gacaacgaca tcggcacctt tctcgcgcat ttcaggcacg tacttgcgca
    10081 cggtttcggt aatatcattc accgtcactt tcccggataa attagcttta tcccagccca
    10141 tgatttgcgg cggcacgacg ccaatatagc caatcttcag cgtctgtttt tttccgtctt
    10201 tatcgaccac ttcggtgtct ttaattaaat acggtgtaaa cattggctgt ttggttctgg
    10261 cgtcaatgac gttggcattt acataaggga atttcgctcc cgccagcgca tttttcaggt
    10321 aatccagacc gtagttaaac tcgtggttgc caagcgtgcc gacggtatag tccagcgtat
    10381 ttaatgcctt atagaccggg tgaatatcac ctgcttttaa acctttcgcc gacatgtaat
    10441 cggccagcgg actcccctga atcaaatcgc cgttatcgac cagtacgctg tttttcactt
    10501 cattgcgggc atcgttaatc aggcttgcgg tacgtaccag tccgaatttt tccgtggcgg
    10561 tgtctttgta ataatcgaaa tccatcatgt tgctatgcag atcagtggtt tccatgatac
    10621 gcagatcgac cgtcgctgca ttcacactgg cggcaatcag cgtggctagg agcgttgcgc
    10681 taaacttaat catcagggac atccttttat catcgggaat acgaaagaaa agggagaata
    10741 aacgtcttac ttatagaaca gtgaagaatg ccacaatttt acgctttgaa aatgatgaca
    10801 ctatcacagt tggcgcattc attaacgata gggtataagt aaaacaataa gttaacaccg
    10861 ctcacagaga cgaggtggag aaatgttaga tcaagtatgc cagcttgcac ggaatgcagg
    10921 cgatgccatt atgcaggtct acgacgggac gaaaccgatg gacgtcgtca gcaaagcgga
    10981 caattctccg gtaacggcag cggatattgc cgcacacacc gttatcatgg acggtttacg
    11041 tacgctgaca ccggatattc cggtcctttc tgaagaagat cctcccggtt gggaagtccg
    11101 tcagcactgg cagcgttact ggctggtaga tccgctggat ggtactaaag agtttattaa
    11161 acgtaatggc gaatttaccg ttaacattgc gctcatcgac cacggcaaac cgattttagg
    11221 tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca aagcgtggaa
    11281 agaagagtgc ggcgtgcgca agcagattca ggtccgtgat gcgcgcccgc cgctggtggt
    11341 tatcagccgt tcccatgcag atgcggagct gaaagagtat ctgcaacagc ttggcgaaca
    11401 tcagaccacg tccatcggct cttcgctgaa attctgcctg gtggcggaag ggcaggcgca
    11461 gctgtacccg cgcttcggac caacgaatat ttgggacacc gccgctggac atgctgtggc
    11521 cgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt acactccgcg
    11581 cgagtcgttc
//
LOCUS       DARNVM010000155        11528 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.155, whole genome shotgun sequence.
ACCESSION   DARNVM010000155 DARNVM010000000
VERSION     DARNVM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.155"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..671)
                     /locus_tag="SI621_003798"
     CDS             complement(<1..671)
                     /locus_tag="SI621_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEI4066456.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNTAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMVTATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGTPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVT"
     gene            complement(671..1024)
                     /locus_tag="SI621_003799"
     CDS             complement(671..1024)
                     /locus_tag="SI621_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066457.1"
                     /translation="MRYRREDADGDYAFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            complement(1024..1776)
                     /locus_tag="SI621_003800"
     CDS             complement(1024..1776)
                     /locus_tag="SI621_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEI4066458.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VAVQPAIKGYEPDSNGINQSTILPLLVDVPVVFPRGGGCTLTFPVKAGDECLVIFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSSTDGVN"
     gene            complement(1885..2226)
                     /locus_tag="SI621_003801"
     CDS             complement(1885..2226)
                     /locus_tag="SI621_003801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066459.1"
                     /translation="MKKIFLLAVLTPTICVGGTLNDFFGLHTSLADNYIIKREITNRA
                     VSNAAMDAVSNGREADFGDELMKEHGDSYGEIALRQLASECGYSFSDSGQSLTTLKKE
                     ECRLVLSENKQ"
     gene            complement(2497..3537)
                     /locus_tag="SI621_003802"
     CDS             complement(2497..3537)
                     /locus_tag="SI621_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066460.1"
                     /translation="MSKNWMRHFELLLVDDKGDGIKISELKVTFNIQKMPATIFNGFV
                     GNFKVYNLSPTTQNRIMQKEFSRIQVIAGYKGQPDAAGNYPDENVGMIFNGDIRFTVT
                     GKDNATDSWIMLQCIDSWEGHLNASVKTTVAAGWKYSDLFSLGMKSFEPYGIESGAVP
                     DMPETVFPRGRVVYQNTSRLMNHIAGQCKANWWYENNLVNIVPEDKYIGVATVLNANT
                     GLIGMPQQTMGAGVNVRCLINPNIKLGGLIRLDQASVYRASLSNDQVAKSPARLDESE
                     SDGNLYVNGLPGMSQPASINTDGDYIVGSIDYTGDTRGQAWYMDLLCLAKGSKSLLNP
                     DTIAKISGVPNA"
     gene            complement(3540..3842)
                     /locus_tag="SI621_003803"
     CDS             complement(3540..3842)
                     /locus_tag="SI621_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066461.1"
                     /translation="MKAVEIPLVADNQTFATTINGSVYHLSVIWRGEYWVLDLADSNG
                     SAIISGIPMITGADLLAQYRYMDLGFSLVVLCDVAGQENPTQFDLGTLSHLYVFTE"
     gene            complement(3846..4496)
                     /locus_tag="SI621_003804"
     CDS             complement(3846..4496)
                     /locus_tag="SI621_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066462.1"
                     /translation="MDFLSVLLQQRTRSIGIIIPDVVITEKHTDALEITEHPVEQPTN
                     AGASGEGAGYISEHAFRRPSEVVMETGFSGGGSLLDFASNLTATSLLGLSPKELYQEL
                     LNLQRNRIPFDVTTGKRIYNNMLIKTLEVTTDKSSENVLLATLTLREVIITSTQSVRV
                     APKNNMTEGVGTSAVQNTGTKTTVPPNNSILKSLPQMAQEGISTVDGYLSNLFLGR"
     gene            complement(4496..6502)
                     /locus_tag="SI621_003805"
     CDS             complement(4496..6502)
                     /locus_tag="SI621_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase catalytic"
                     /protein_id="HEI4066463.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSSMPYYRANQYAQMLG
                     IDENTLMAMRRGVGQFSAQYSEMVKAIGFNADQAALSSNRFMTSLKSLGEMAGMARDK
                     IGSNLADGLAGQIDNLRKKIIENFPKIEVTITKVIKGILWLGEIVGRVAFRIVDGVGD
                     IIEWWGKLDAETKTLIEVIGGLVVAMRILNSTFWMSPIGLITGLIVALGLLWEDYKTW
                     KEGGNSLIDWEKWQPAIDKAKDAITWFRDKLLELKDSVGGWQKSLEILGTFIAGVWIS
                     KVLGAFGKISGLPIPPWLKLWALYAGYIVSDRENIADSAKSSLRYTKRIIGDTLAAIG
                     IKTDIGRRDVSEVREWPAWMDWLHGGPGKVIRQGQSNGVVHGSNVQPDIPGGGTLADR
                     NNNPGNIRPVSGKGFRFFESALEGWEAMKNQLMRYFTGKTTGRALQTIQDIVSTWAPA
                     GDNNDPKKYAQDVAKWMGVSPNAILNLTDPRTMGALMQSMARKEGYSNWNSPLAYKVA
                     AGSLNQQTVINVHGVNNPQEAANLIADKQGAVNARAVQQLKGPA"
     gene            complement(6680..7132)
                     /locus_tag="SI621_003806"
     CDS             complement(6680..7132)
                     /locus_tag="SI621_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066464.1"
                     /translation="MEFEIKGVKYRAAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTDAIIHPCLAVVSRRHMDGWV
                     PVFTRGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            complement(7136..7576)
                     /locus_tag="SI621_003807"
     CDS             complement(7136..7576)
                     /locus_tag="SI621_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HEI4066465.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            complement(7587..8732)
                     /locus_tag="SI621_003808"
     CDS             complement(7587..8732)
                     /locus_tag="SI621_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HEI4066466.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKAAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            complement(8736..9299)
                     /locus_tag="SI621_003809"
     CDS             complement(8736..9299)
                     /locus_tag="SI621_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066467.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(9274..9663)
                     /locus_tag="SI621_003810"
     CDS             complement(9274..9663)
                     /locus_tag="SI621_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HEI4066468.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(9650..10204)
                     /locus_tag="SI621_003811"
     CDS             complement(9650..10204)
                     /locus_tag="SI621_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066469.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATMELARRAAGESPGTELA
                     KPLIDTGQYRRAITHVVRDKNADS"
     gene            complement(10201..10608)
                     /locus_tag="SI621_003812"
     CDS             complement(10201..10608)
                     /locus_tag="SI621_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HEI4066470.1"
                     /translation="MSKNQSLPAVSDFRRDFPQFADPAKYPDAQIGFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            complement(10574..10942)
                     /locus_tag="SI621_003813"
     CDS             complement(10574..10942)
                     /locus_tag="SI621_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066471.1"
                     /translation="MAKEKLVTIHVHTPFTLTLGDQSKQEFGRGRHNVPEEVASHWFT
                     QAHSELSESVISDTDDLQPIIDGLQAQIADKDKLIADLKDALLKLQEQNDSLQAQITA
                     ARTGGNGAKDVKESKSASGK"
     gene            complement(10983..>11528)
                     /locus_tag="SI621_003814"
     CDS             complement(10983..>11528)
                     /locus_tag="SI621_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HEI4066472.1"
                     /translation="QMDNDEQVYVGDSALNLKGLVTLDGVPVNNAAKTWATSTPDEIR
                     ASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAGNQSLLTYLQTNTISYH
                     QNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPLQSVPVQYRGLYQIVTY
                     YGKLGAVEPVYKETLSYVDGI"
BASE COUNT         2824 a         3186 c         2625 g         2893 t
ORIGIN      
        1 gttacaccgg taacattaga aacagcacca tccagtgctt caaatggggt tattgatggc
       61 aacgcaacac tttgcgactg gcggatacgt aactccgcgt cagtttctgc cggagtgccc
      121 acggtagccg cagcaggatt agttaccgaa acccagccac gggttggcgt atttatttca
      181 gtgatagttc cagccagcgc cgccactgca ccactgacgg aacatgttgc agtcaccatc
      241 actgtaccat ccacgccgac caccactgaa gcaggcaaac gccatatcac attattactg
      301 tctttcacgc tgccattaat gatggttgtt ccggcagttc ctgtaagaag caaatcaacc
      361 gtagaattcg tcgcgccttt acgtgaaata ccatttattt tcacgttact ggtcagtgca
      421 gccccatagc cggttgccgg tgaaaaacag ttgtagacag ttatcgccgt attattggca
      481 tcatgaatcg ccaacgccat cagagccacc atctggccgt ctttgctgtc cggttcgagg
      541 taggcatcac tgccataaat ctgctgaaaa tagctaatca gggtgctgag tatcgtctga
      601 taatcaggcg cactgatccc ctccgcggtt acctttgcag ataaaccgag agaatcaagg
      661 ttcagagcca ttacgcctcc gatgtaacag tcgttattcc ataaagagtg tcgatttcag
      721 cggaaaacat gacacgtcgg gtcgtggtat ccaccgtcgt attgaaagag aggattgatt
      781 taacgccccg cgtttcgagg atgcgcttac ggatcgccag gttgtaggtt tccggcttct
      841 gcttaccgag cacggactgg atccacggtg ttccctctgt ggtatcaaga aaccattgcc
      901 cataccacaa ttcgaatcgc gttttcacag cctgcgccac ggcctcaggc gagttaatca
      961 gccaggtgtc atcaccgctg ccaaaggcgt aatcgccatc ggcgtcttca cgtctgtatc
     1021 gcatcagttt accccgtcgg tactgcttcc accgcgctga acaccaccat gagtgtgtgt
     1081 atcatcgatt ggcttgccat tagccttcac gctccccaaa aactcaacag caccagtgat
     1141 ttttgaagcc acaccagaaa caacagaccc caccatgccc cccatccagg ttaacaggcc
     1201 atgaatggtt actttctcag aaaaatcagc cagaggggca accacatcaa gaccacccgg
     1261 agcgacaatt ttaattttcc tggtatcagg attaagctca aaataggtgc tgccgtcgtc
     1321 actacgcaac tgtgtggcac tggtattaat accgctaatc ttccttgcct gcgactgggg
     1381 accgacaata caaaacgcat ccgataaatc atgcattctg tcatcgacag gctcctgtat
     1441 cccgccactc tgccaccaga aatcaataca acgatcggca aaaatcacca aacattcatc
     1501 accggcttta accgggaacg ttagcgtaca gcctccgccg cgcgggaata ccactggtac
     1561 atccaccagc aggggtaata tcgtcgactg gttgattcca tttgagtccg gctcatagcc
     1621 tttaatcgca ggttgaacag ccactgttac cgtgtccgga tcaaatgact ggacgatgcc
     1681 gggcatagaa acacgcagcg ccgacatgac agagccagca agtcttacat cggcctgttc
     1741 tttgctacca agttgagcgc ttaaagaaac gggcattcat atgtctccag caagtaaaaa
     1801 acccgccggg tggcgggttt tatgtagcta gttttctttc catggcgata gaagaaatta
     1861 ccgtggaaag ttaatttagc gaactcattg cttattttca gatagcacca atctacattc
     1921 ttcttttttt agagtcgtaa gagattggcc gctatcggaa aaagaataac cgcactcgga
     1981 agcgagttga cgcagagcta tttcaccata actatcacca tgttctttca ttaactcatc
     2041 accaaaatcc gcctcacgac cattagatac agcgtccata gccgcattag aaacagccct
     2101 atttgtgatc tctctcttga tgatgtagtt atcagccagt gaggtgtgca gcccaaaaaa
     2161 atcattcagc gttccaccta cacaaattgt tggagtcaac actgcgagaa ggaatatttt
     2221 cttcattaca aatgtctcct atagctgatt tcttaaaaaa attaattaca gtacgttgtg
     2281 ccagtattgg tcgtctggca ttcacgatgt actgcattcc cattaatcgt tcctgtggta
     2341 tacgtagtgc ctgtattggt tgtataagat tctgttctaa ctgtagaacc actactgtct
     2401 gtaccagcac agtaagttgt tcctgtattt gaagtggtgc atgttactgc agccatagaa
     2461 gacaaagtca caaaatatac cagtaataag atccatttaa gcatttggta ccccacttat
     2521 tttcgctatc gtatcaggat tgagtaaact tttactccct ttagccaggc aaagcaggtc
     2581 catataccac gcctgccctc gggtgtcgcc agtataatca atgctgccca caatgtaatc
     2641 accgtcagta ttaatgctgg caggctgtga catgcctggc agaccgttaa cgtagagatt
     2701 accgtcgctt tcagactcat ccagtcgtgc tggcgatttc gctacctggt cattactcaa
     2761 agaggcacgg tatacagatg cctgatccag acgaataagc ccaccgagct taatatttgg
     2821 attaatcagg catctgacat ttacgccagc tcccatcgtc tgctgtggca taccgataag
     2881 cccggtgtta gcattcaaca ccgtagcaac accaatatat ttatcttcag gaacaatatt
     2941 taccagatta ttttcatacc accagttagc tttacactgc cctgcgatat gattcatcaa
     3001 ccttgatgtg ttttgataaa caacgcgccc tcggggaaat accgtttcag gcatgtcagg
     3061 aactgctccg gattcgatgc catatggttc gaatgatttc atacccagac tgaaaagatc
     3121 gctgtacttc cagccagctg ccactgtggt tttcacactt gcgttcaggt ggccttccca
     3181 gctgtcaata cactgcaaca tgatccaact gtctgtggca ttatctttac cagtgacagt
     3241 aaaacggata tctccattga atatcatacc aacgttttca tcaggataat tacctgctgc
     3301 atccggttgc cccttgtatc cggcaataac ctgtatacgc gaaaactcct tctgcataat
     3361 ccggttctga gtggtaggag acaggttata aaccttaaaa tttccaacaa atccattaaa
     3421 tatggtcgca ggcattttct gaatattgaa agtgacttta agctcagaaa tttttatccc
     3481 gtcgccctta tcatcaacaa gcaataattc aaagtgacgc atccagtttt tcgacattgt
     3541 tactccgtga aaacatagag gtgtgagagc gttccaagat cgaattgcgt cggattctcc
     3601 tgccctgcca cgtcgcagag caccaccaga gaaaaaccca gatccatata tcgatactgt
     3661 gccagcaggt cagcccccgt aatcatcggt atacctgata taatggcgga gccattgctg
     3721 tcagcaagat ccagaaccca gtactcgcct cgccagatga cagacaggtg ataaaccgaa
     3781 ccgttaattg tggtggcaaa agtctgattg tcagcaacca gtgggatttc tacggctttc
     3841 atgaatcacc ttcccagaaa taaattgctc aaatacccat caacagtgga gataccttct
     3901 tgcgccatct gtggcagcga tttcagaatg gaattattcg gcggcaccgt tgttttggtg
     3961 cctgtattct gcacagcaga cgttccgact ccctcggtca tattgttttt cggggcaacc
     4021 ctgactgact gcgtggaggt aataattact tccctgaggg taagtgtcgc cagaagcaca
     4081 ttttcactac tcttatcggt cgtgacctcc agcgttttta tcaacatatt gttgtaaata
     4141 cgcttgccgg ttgtcacatc gaaaggaata cgattccgct gcaggttaag cagttcctga
     4201 tacagttctt tcgggctcag ccccagtaaa ctggtagccg tcaggttact ggcaaaatca
     4261 agcagcgatc cgcctccgga aaaaccggtt tccatcacaa cctcagaagg gcgcctgaat
     4321 gcgtgttctg atatataacc agcgccctca ccactggcac cagcattcgt gggctgttca
     4381 acaggatgtt ccgtaatttc cagggcgtca gtgtgctttt cggtaataac cacatcagga
     4441 ataatgattc ctattgagcg ggttcgctgc tgcagtaaaa cagataaaaa gtccattacg
     4501 caggtccttt caattgctgt accgccctgg catttacagc cccctgcttg tcagcgatca
     4561 gattagccgc ctcctgaggg ttgttaactc catgaacatt tataacagtc tgctggttaa
     4621 ggctgccagc ggcgacctta tacgccagcg ggctgttcca gtttgaataa ccttctttgc
     4681 gcgccatcga ctgcatcaat gctcccatag tccggggatc tgtaagattc aatattgcat
     4741 tcggtgatac tcccatccat ttcgcaacat cctgtgcata ctttttcgga tcgttgttat
     4801 cacctgccgg ggcccaggta ctgacaatat cctgaatagt ctgtaatgcc cgtccggttg
     4861 ttttcccggt aaagtaacgc atgagctggt ttttcatcgc ctcccagccc tcaagcgctg
     4921 attcgaaaaa acggaaccct ttaccgctca ccgggcgaat gttcccgggg ttattgttgc
     4981 gatcagcaag agtgccgccg ccggggatgt cgggctggac attggagcca tgcactacac
     5041 cgttactctg tccctgccgg ataactttac cgggtccccc gtgcaaccag tccatccacg
     5101 caggccattc acggacctca ctgacatccc tgcgtccgat atctgttttt ataccaatag
     5161 cagccagcgt gtcgccgata attcgcttcg tatacctcag tgaagatttt gcgctatcgg
     5221 caatattttc tctgtcacta acaatataac ccgcgtaaag cgcccacaat ttaagccagg
     5281 gtgggatcgg caaacctgat atttttccga aagcccccag aaccttggat atccagacac
     5341 ccgcgatgaa tgtaccgagg atttccaatg atttttgcca tccccccacg ctgtctttca
     5401 gctcaagtaa tttgtcgcgg aaccaggtga tcgcatcctt cgctttatct attgccggtt
     5461 gccatttttc ccagtcgata aggctgttac cgccttcttt ccatgttttg tagtcttccc
     5521 acaagagacc gagagccacg atcagaccag taatcagccc tataggtgac atccagaaag
     5581 tagagttaag tatccgcatg gcgacaacca ggccgccgat aacctctatc agggttttcg
     5641 tttcggcatc cagtttcccc caccactcga tgatatctcc gacaccatcg actatccgaa
     5701 atgctacccg cccgactatc tcacccagcc agaggatccc ttttatgacc tttgtgatgg
     5761 tgacttcaat tttgggaaaa ttttcaatta tctttttgcg caggttatca atctgccccg
     5821 ccagtccgtc cgcaagattc gatccgattt tgtcccgcgc catcccggcc atttcaccga
     5881 gcgatttcag cgaggtcata aaccggtttg acgataaggc agcctgatcg gcattaaatc
     5941 cgatcgcttt caccatttct gaatactgag cgctgaactg ccccactccg cgacgcatag
     6001 ccatcagggt attttcgtca atgcccagca tctgcgcata ctggttagcc cggtaatacg
     6061 gcatgctgct gagtttctgt ccaacgcccg taaagatagc agccatgtca cgcatgttac
     6121 cgctggcatc acgggtctgt acgcccaggc gattcagaaa gccttctgct ccgggattgt
     6181 tacgaataaa ccgggagagg ctttccagag aagatcgcgc agcgtccaca ctgccgccaa
     6241 cctgcgaaac cgcatagcca atagactgaa ttccctggac cgtcgcgccg gtgcgctgtg
     6301 acgcccagta aagattatcc agaccggagg cgatcttagc cgtgaaggcc accacggaca
     6361 gcgcagcccc ttcaacagcc agccccattt tgatgacatt tgcagttgta ccggcgagga
     6421 cagaaccgaa ctttttcgct cctgcatcat ccacactgaa gccaagcgag acgaggaaat
     6481 ctttaatagt ttcagcgttc attatcctct ctccatttct caatgcgccg ctggttatcc
     6541 gcttttaccg ccagatggtc attcaagaga gcaatgtcgt acaaatcgac agagccatct
     6601 ttaagtgctg tataaggaat taacccggcg tcaaccggat tgagaaggta ggacagcccg
     6661 tccggcaggc tgttaaacgt cagccctgtt gcaggctctg cgtcgtgctg gtaaggggtg
     6721 taggcaaaaa atttcccagc gaatcggcga ccacccgcgc caccagctgc agcatgacca
     6781 gcaggtcaat atcatcaaac atcagttcgc cccgggtaaa taccggcacc catccgtcca
     6841 tatgacgccg cgataccacc gcaagacagg gatgaataat cgcatcggtg tcatcttcgg
     6901 tcagggaaga cagttcctca gcgatacgcg ggagcatggt ttcaaacacc ggttttaact
     6961 gctcgaattt cacggtgtcg attttgccat cagcaggcaa acgggagcga atgctcccga
     7021 aatctgacat catccccgcc agtaccggaa gaagtttgcg ggtcactttc agctggtcaa
     7081 aaacgctgag ttttgccgcg cgatatttca cgcctttgat ttcgaattcc atgtattaaa
     7141 actccccgag aacctggtca atcttgccgc agtcaaacac ccacggcatc gtattaccgg
     7201 ttttagcgtt ggcattatcc ggttgtttct ggaacgcaac actacgtgcc gtgatgatgt
     7261 cgccgctgac cttgttgcgg atcacaataa cgttattccc ccatgtggca gaagactggc
     7321 tctgtgcgtt atacgccagc gacaattttt tatttgtcgg tgatgtcttc agaaggttaa
     7381 cggtaatcgt cccgctttta tctgcatgga gactgtgcat cacttcgcca tcagcaccga
     7441 tggtcatggt gtttttagga ccgcccatcg caaccacaat cccctcttca gaacttgcag
     7501 aaccgtaccc gaggtcaatc gaaccggtcg gcccggtcag cgtcgcagtg acatccataa
     7561 aagaataggt agacattcac ttccccttag cgaacaacgt taatctgtac gtcagcgtaa
     7621 tgaaccgcgc ctgcaagttt tattgcagcc tgaatcaccg gagccttacg ggcttcacgt
     7681 tctgattgag cctgttcatc cagcggctga gcgtatacgt aataaccttt gggcagtgtg
     7741 tcacctgatg acaactgacc aaggtcgccc ccgttccata cgcccggagc aatcagtcca
     7801 ttctgaacgg cttgatccag tgatttttca acatttgata acagtcgggt aataccggct
     7861 tcagtctggg gaactttcgt ggtgctggta taaagcaggt tatagaggtt ggtctgcaca
     7921 taattctgta accagtccag gccgtggcgt tcatcaaaga aatcgccgtt agccatcact
     7981 ccctgctgga ggatagctgt atcattctgg tagtacacga acacattgca gttttttgca
     8041 tcaagtgccg atgcctggct gactgtcagt gtttcatacc cgacacccgg ctcctgctta
     8101 aacttgagcg taatcgcggt attactgcca ttgaaattaa ccgtgaatgc ccggccaaat
     8161 gcagataacg cagcgtattt attacccgat gaatactgaa taaaactgcg tgaatatccg
     8221 gcggctttca gttttgatgc caaatcatcg ctggatgcag tctgcaggca tttctcatcg
     8281 cttgtcgtaa tcgccagaat acggcttaca gaagaggatt cgatcgccgc agccactttc
     8341 agccagtctg catccggaat atcttcatcg tctgcaatcc ccagcccata ccatgaagta
     8401 taatcaagca tggcattcac agcctgctcc agcgtctcag gcgtggcctg ttcgctgtct
     8461 cccttcgttt tcacccaacg accaacaaaa acctcctgag gtttcggtga ttgagagaaa
     8521 aacacctgcg cagccttata ttctggtgat tccacgccaa aatcttttcc aatatcttcc
     8581 gcggcagaat aacggcgaat gcgctcactt accggaatga ttgtggacgg gccgagaatg
     8641 agtaatgcac caaaatttcg ccctgatgct gcacgcggcg acatgatcac atcaacatta
     8701 acaacgtttg atacaggcaa gccctgtgcc atagcttaat ctccgaaaaa gatgactggt
     8761 gcttccacca gcgatttaat accgtactca cgcacaacct tccggcgcag gcgcaccgtc
     8821 atatcgtagc ggcggaccca ttgctgatta ataagttcag ggaagggagt cagacctgtg
     8881 taatcgccaa gagacagccc cagcgcattc agtgctgcat tgttctgcgg cacagatata
     8941 ccgtcacgaa accgggacgc atacaccatc cccgccggtc cataaaacga agccatacac
     9001 tcaatcgttt catgccgcca gagctgagag ccatcatcgg tctgtctggt gaatgccgga
     9061 ctgtcatcac ctgaccatcc gataacccca aacgcacacc agttcgtttc aaccggtagc
     9121 agtggcggct gctctttctg ccagcgcggg cgaaccatcc cggcagacag accggaaacg
     9181 ttacgcatcc actggcttaa cagcctgtcg agcgcttcgt cataatccgg atcgccactg
     9241 gttggtatta accatccgcg ctctgtactg gtgttattgc tcaaccggag ttcccccatc
     9301 aaacggcatc aactcacaat gcgcctgaac gaatccggcc ccataagctg tatacgggtc
     9361 gacgaaggtc acacgataat cacggccctg atacgtcacg atatcggcat cacggccagt
     9421 ctgtccctgt gtcagtcgct cagtcgtcac aatcagaatt gcaccactga ttacctgccc
     9481 ggcctgcata cgacggtttt ccagagagcg atcaacagta acgactccgg caaactgctt
     9541 tttaacttcg ctgtcgctgc cgatcccgtc ctcatccacc gtttgcacac ggcgtgttac
     9601 ccacaaattg aagtcgcaaa aatcggggtc aaaaagcaca tctgttacat caagagtcgg
     9661 catttttatc cctcacaaca tgggtaatag ctctgcgata ttgcccggtg tcaattaatg
     9721 gtttcgccag ttcggttccc ggagattcgc cagcagcacg ccgggcaagt tccattgttg
     9781 cccccttgcg cccccgacga gcccgggctt caacagtact gtcagcaagc ggcgtaaagc
     9841 cggtaatggt catgtaacgc ctgacgccat tagcggccag cgttccggca cggttgagtg
     9901 cgcgttctgc tcccgcagca ttaccatcaa gtgcagcctg cgccgcggct ttgagctgcg
     9961 gcaccgtctg ctcttctgcc gatttaacgc cggggaccag gtgaggtcgt ggcgggatgt
    10021 tctgctctgg tgagccgtat tcgttgaggt aaccgatgcc cgcattacca aacggaacat
    10081 catcccgctc gctgtcttcc gaagggatgc cgaccagcac atcttttttg gttaacgacc
    10141 tgagcgcatc cagaatggcc ttagcgttat ccaccctcgt tgttacaccg cttttgaaac
    10201 tcatagctgg cgaccacctg caccgaacat cgtgatcaac tgataaaatt cagcgccata
    10261 tcgggtgtta ttccagaaac ctgcatcagg attcagcgtc gcgctggtgt catagctgac
    10321 gcttacctta tccacggact ttgaggactg aacaccattg gttgaaccgc ccggaccacc
    10381 agccagcatc gctctgctgt cagccgccca gagcgtcatg tagtgagcaa cgaacaaccc
    10441 ggcaaagtac ggaaacaact ttttgccggt gacattttcg ctcagcagtt catcggccag
    10501 attcagacgg aacccaattt gggcgtcggg atattttgcc gggtcagcaa attgcgggaa
    10561 gtcgcggcga aaatcactta ccgctggcag actttgattc tttgacatct ttagccccat
    10621 taccgccagt ccgggcggca gtaatctgcg cctgcaggct gtcgttctgc tcctgcagtt
    10681 tgagcaatgc atctttcaga tcggcaatca gcttatcttt gtcggcaatc tgcgcttgca
    10741 ggccgtcgat aatgggttgc agatcatcgg tgtcgctaat cacgctttcg gaaagctcag
    10801 agtgcgcctg ggtgaaccag tgcgacgcga cctcttccgg tacgttatgc cgtccccggc
    10861 caaactcctg ttttgactga tcgccgagcg tcagcgtaaa cggggtgtga acatggatgg
    10921 taaccagctt ttctttcgcc atttcagttt ccttcaggcc cctttcgggg ccattctggt
    10981 tatcagatac cgtccacata ggacagagtt tctttataca ctggctcgac tgcacccagc
    11041 ttgccgtagt aagtgacgat ctgatacagg ccgcgatact gcaccggcac gctctgaagc
    11101 ggaaccagcg ggtagcggac gtatttttta tcgttggtgt acgcaaccat gcgatcctta
    11161 ttccccacac cacggccttt cagccattta accgcgcgga tattcagcgg aacaccgttc
    11221 tggtgatagc tgatggtgtt ggtctgaagg tacgtcaaca gggactggtt acccgcagat
    11281 gaaacgatga tgctggacaa cagagcaaac tgttcaggcg ggatcagcaa atcacgcggg
    11341 accacagagt aaccagaagc ggcccacgca tcagacagca cctggttaat gcttgcgcgg
    11401 atttcgtccg gtgttgaggt tgcccacgtt ttggcagcgt tgttgacagg cacgccgtcc
    11461 agggtaacaa ggcctttcag gtttaatgcg gaatcgccaa catatacctg ttcatcgtta
    11521 tccatctg
//
LOCUS       DARNVM010000156        11317 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.156, whole genome shotgun sequence.
ACCESSION   DARNVM010000156 DARNVM010000000
VERSION     DARNVM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.156"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            141..5561
                     /locus_tag="SI621_003815"
     CDS             141..5561
                     /locus_tag="SI621_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HEI4066473.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLHKSSGLTTKF
                     SRLTLGVLLALSGSVSGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNENSKGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRIGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAIVGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNPTGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGKVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSLFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGAGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMNVGTTGTGTLTLTNNGTLN
                     VEGGEVYLGVFAPAVGTLNIGAAHGEAAADAGFITNTTKVEFGLGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVMHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGITGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGTLTFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSAQTVETFTG
                     LMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRVVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNKDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTVGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5745..6011
                     /gene="hipB"
                     /locus_tag="SI621_003816"
     CDS             5745..6011
                     /gene="hipB"
                     /locus_tag="SI621_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HEI4066474.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6011..7333
                     /gene="hipA"
                     /locus_tag="SI621_003817"
     CDS             6011..7333
                     /gene="hipA"
                     /locus_tag="SI621_003817"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HEI4066475.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK"
     gene            complement(7731..8630)
                     /locus_tag="SI621_003818"
     CDS             complement(7731..8630)
                     /locus_tag="SI621_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4066476.1"
                     /translation="MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALKLLEE
                     YIGHPLFVRTTRRIELTETGQLLLEQTAAAVESLENSLESIRDQHQEPSGMVRITLSR
                     FAYLLILKPAMAEFCQQYPGIQLEISVYDGTVNIIKERFDLGIRFGDILEGGVVARPL
                     MKPFREGLYASSAYLSRHGTPEVPADLCHHQLIGYRFITNNRILPLLLNDRGEQLTVE
                     MPGQLISNDIDVMADGIRNGLGIGRLFEPVWQLQPDRERFIPVMESYWKTYPPVYLYY
                     PKNAGKTKRVKALIDFLISTTGR"
     gene            8770..9078
                     /locus_tag="SI621_003819"
     CDS             8770..9078
                     /locus_tag="SI621_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEI4066477.1"
                     /translation="MRILCLDIPAPGASLEKYAPHLNAEALHAWGLYKSGFIRDIYFR
                     QDRPGVAIFLECDSVDEAMNVMAEFPLAKAGLLSFECIPLGSFINWENLFADEFKNKE
                     "
     gene            9087..9776
                     /locus_tag="SI621_003820"
     CDS             9087..9776
                     /locus_tag="SI621_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEI4066478.1"
                     /translation="MKPAYKPVLCVVSSHPIKGASGVPTGFFLAELTHPLKVLEDAGI
                     KTTIASIRGGQPPVDGFDLSDPVNAWYWNETDFQQRLATTPALSELKGADYSAVFFAG
                     GHGTMWDFRDNQDAQRIIREVYESDGIVSAVCHGPAALVDAKLSSGEYLIKGKNVAAF
                     TNKEEEEVHTTDVVPFLLETALREHGALYHEAPNWSENVVTDDRLITGQNPASAHGVG
                     VALLNALRRTA"
     gene            9850..10797
                     /locus_tag="SI621_003821"
     CDS             9850..10797
                     /locus_tag="SI621_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4066479.1"
                     /translation="MKTLPPVALGTWSWGTGFAGGDTVFGNHLSDTQMAEVFTAAMSK
                     GLNLWDTAAVYGMGSSETALGALVRQFPRENIILSTKFTPQIADKQSAQPVSDMLEAS
                     LGRLGVDAIDIYWIHNPLDVEKWTPGLIPLLQSGKVKRVGVSNHNLAQIRRANEILNA
                     SGYSLSAVQNHYSLLYRASEEAGILDYCRQNNITFFAYMVLEQGALSGRYDSNHPMPA
                     GSGRAESYNAVLPQIERLTAAMKKMGAERNASVAQIAIAWAIAKGTLPLVGATKVHHV
                     LDAACASDIQLRDEEIILLEQLAAETRVDTRGAWEKPMV"
     gene            10900..11055
                     /locus_tag="SI621_003822"
     CDS             10900..11055
                     /locus_tag="SI621_003822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066480.1"
                     /translation="MKIICLEENYLDSELGRACMPVALEQAPFLGDWGKTVADGHNPD
                     RLPAFPL"
     gene            11015..>11317
                     /locus_tag="SI621_003823"
     CDS             11015..>11317
                     /locus_tag="SI621_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEI4066481.1"
                     /translation="MVIILTDSLLSRFNKLNVPLYLHPGLPLKSVQQAYFTGFSAEVN
                     SRLSMFAWGWHHEAGIHLLRLMLSGAFDKYPHLQVISGHWGEMLPFWLQRLDDSLPL"
BASE COUNT         2989 a         2588 c         3027 g         2713 t
ORIGIN      
        1 ttagcaaatt agccaaattg acaccttccc acctccaacc aacactcaat acagacctct
       61 gacaacttaa taagtttcat ttttaacgtc tgatgactca tctctttcgt ggctgtattg
      121 tttgtatatg gagcgtttat atgaatagaa tctatcgcgt gatatggaat tgcaccctac
      181 aggtatttca ggcctgctcg gaattaactc gcagggtagg taaaacatcg acggttaatt
      241 tgcataaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt gttttgctgg
      301 cactaagcgg ttcagtgtct ggtgcgagtc tggaagttga taatgatcag attaccaata
      361 ttgatactga tgttgcttat gatgcctacc tggttgggtg gtatggcact ggagtgctta
      421 atattttggc tggcggtaat gcctccttaa ccactattac taccagcgtc attggtggta
      481 atgagaactc gaagggcacc gttaatgttt tgggtggcac ctggcgattg tatgatagcg
      541 gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg aatattaaac
      601 agaagggtca cgttgacgga ggatatttaa ggataggttc ttcgacagga ggcgtcggga
      661 cggtcaatgt tgagggagag gactcggttc tgacgaccga attattcgaa atagggagtt
      721 acggtacagg ctcattaaat attacggata agggttacgt cacgagttca atagtcgcca
      781 ttgtaggata tcaggcgaac agtaatggta aggttgtcgt tgaaaagggt ggcgagtggc
      841 taataaaaaa taatgattcc tcaattgaat ttcaaattgg caatcaaggg acgggggaag
      901 cgactattcg cgagggggga ctgattacgg ctgaaaatac gattatcgga ggcaatccca
      961 ccggtatcgg aaccctgaat gtgcaggatc aagactctgt catcacggta cgcagactct
     1021 ataatggata tttcggtaat ggcacagtca atatttccaa taatggactg attaataata
     1081 aagaatattc attggtgggc gttcaggacg gttcccacgg tgtcgtcaac gtgaccgata
     1141 aagggcattg gaatttcctc ggaacgggcg aagctttccg ctatatctat atcggtgatg
     1201 ctggcgacgg tgaacttaat gtctcgagtg aaggcaaagt agattcggga attatcactg
     1261 cggggatgaa agaaacaggc acaggcaaca ttactgttaa ggataagaac tccgttatca
     1321 ctaatctcgg aactaatctt ggttatgacg gccacggcga aatgaatatc agtaatgagg
     1381 ggcttgttgt cagcaacgga ggaagctcac tcggttatgg agaaaccggc gtcgggaagg
     1441 ttagcatcac cacggggggg atgtgggagg tcaataagaa tgtctatacc actattggtg
     1501 ttgcgggcgt cggaaacctc aatatcagcg atggcggtaa gttcgtatcg caaaatatta
     1561 cttttttggg cgataaagca agcggtatcg gcacactgaa cctgatggat gcgacatcat
     1621 tgttcgatac tgtaggtatc aatgtcggta attttggtag cggcatcgta aatgtcagta
     1681 atggtgccac tcttaattca acgggctatg gatttatcgg aggaaatgcc tccggtaagg
     1741 ggatagttaa tatttcaacg gatagtctct ggaatttaaa aacgtcatct actaacgccc
     1801 aattgctaca ggtcggtgta ttaggcgcgg gtgaactgaa tattaccacc ggaggtatag
     1861 ttaaagcgcg tgatacacaa atagctctca atgacaaaag taagggcgac gtgcgggtgg
     1921 atgggcagaa ctctcttctt gaaacattca atatgaacgt agggacgact ggcacaggta
     1981 cgttaaccct gacgaataac ggtacgctga atgtcgaagg tggagaagtt tacttaggtg
     2041 tttttgcgcc tgctgtagga acgctaaaca ttggtgctgc tcacggtgag gcggcggcag
     2101 atgccggatt tatcaccaat acgacgaaag tggagtttgg tcttggcgaa ggcgtttttg
     2161 tctttaatca taccaataac agtgatgccg gctaccaggt cgatatgctg attacgggtg
     2221 acgataaaga cggaaaagtg atgcatgatg caggccatac ggtgttcaat gcagggaata
     2281 cttatagtgg taaaacgctg gtcaatgacg gcctcctgac tattgcgtca catacggcag
     2341 atgggataac gggcatgggt tcgagtgaag taaccattgc aagccccggt acgctcgaca
     2401 ttctcgcatc aacgaacagt gcaggagatt acacgctgac caatgcgctc aaaggcgatg
     2461 gcttgatgcg agtgcagctg tcatcctccg acaaaatgtt tggctttacc catgcaacag
     2521 gaactgaatt cgccggtgtt gcccaactga aagacagtac cttcacactg gaacgcgaca
     2581 acaccgctgc gcttactcac gcgatgcttc agtctgacag tgaaaatacc acatcggtaa
     2641 acgtgggaga gcaatccatt ggtggactag ccatgaatgg cggtacgctc actttcgata
     2701 cggatatacc tgctgcgacg ctcgcggagg gatatatcag cgtcgatacg ctggttgtcg
     2761 gcgcgggtga ctacacctgg aaaggccgta actatcaggt aaacgggacg ggcgacgtgc
     2821 ttatcgacgt gcctaaaccg tggaatgatc ccatggcgaa taaccctctg acgacgctca
     2881 atttgctgga acacgatgat agtcatgtcg gcgttcaact ggtgaaggcg caaacggtta
     2941 ttgggtcggg tggctcatta acgttacgtg atttacaggg cgacgaggtg gaagcggaca
     3001 aaacgttaca tattgcgcaa aacggaacgg tggtcgccga gggtgattat ggattccgcc
     3061 tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg ctgaacatcc
     3121 atggtgggca aaagctgacg ttagccgaac atggcggagc ctatggcgca acggccgata
     3181 tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa tacggtgcga caggtttcgc
     3241 tttccaacgg tcagaacgac tatcaggggg caacctacgt tcagatgggg acattacgta
     3301 ccgatgcgga tggtgcactt ggcaacaccc gggaactgaa catcagcaac gcggccatcg
     3361 tcgatcttaa tggatcggcc cagacggtag agacattcac cgggctgatg ggttcgactg
     3421 ttttgtttaa agaggggtcg ctgacggtaa ataaaggtgg gatcagtcag ggtgaactga
     3481 caggtggcgg aaacctgaat gttacagggg gaacgctggc tatcgagggg cttaatgcac
     3541 gctacaatgc gttaaccagc gttagcccaa atgcggaagt cagcctcgat aatacgcagg
     3601 ggttaggcag aggaaatatt gccaatgacg gtctgttaac gctaaaaaac gtgactggcg
     3661 aactgcgtaa tagcataagc gggaagggta tcgtgagcgc aacagccagg acagacgtag
     3721 agctggatgg cgataatagc cgcgttgtgg ggcaattcaa cattgataca ggcagcgcgc
     3781 tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt tatcaataat ggcctgctca
     3841 ccatctccac tgagcgtagc tgggcgatga cgcacagtat cagcggtagc ggtgatgtga
     3901 caaaactggg taccgggatc ctgactctta acaaagattc cgcggcgtat cagggtacga
     3961 cggatatcgt gggtggtgaa attgctttcg gttccgactc tgccattaat atggcaagtc
     4021 agcacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcaggtgatg
     4081 tgaacgttat gccagggggg acactgcgtg tcgctaaaac cactgtcggc ggtaacctgg
     4141 agaatggtgg cacggttcaa atgaacagcg aaggggggaa accggggaat gtactgaccg
     4201 ttaacggtaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg ctgggcggcg
     4261 ataattcgcc caccgataag atgaacgtga aaggcgatac ccaagggaat actcgcgttc
     4321 gggttgataa cattggcggc gtcggtgcac aaacggtcaa cggtattgaa ctcattgagg
     4381 tcggcggtaa ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa gctggggctt
     4441 acgtctacac gctggctaaa gggaagggga atgacgagaa aaactggtat ctgaccagta
     4501 aatgggatgg cgtaacgcca gcggatacac ccgatcccat caataatccc cctgttgtgg
     4561 atccggaagg cccatcagtt tatcgcccgg aggccggaag ctatatcagc aacattgccg
     4621 cagccaactc gctgtttagc catcgcttac acgaccgtct gggtgagccg cagtatacag
     4681 attcactgca ttctcagggt tcggcaagca gtatgtggat gcgtcatgtc ggggggcacg
     4741 aacgtttcag gaccggtgac ggccagctaa atactcaggc taaccgctat gtattgcagc
     4801 taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat cttggcgtga
     4861 tggcaggcta cgccaatcag cacagtaata ctcagagtaa tcgtgtgggt tataaatcgg
     4921 atgggcgcat cagcggttac agcgcaggac tgtacgcgac ctggtatcag aacgatgcga
     4981 ataagaccgg cgcttatgtt gacagctggg cgctgtataa ctggtttgat aacagcgtca
     5041 gttccgataa ccgttctgct gacgactatg attctcgcgg tgtgacggcc tctgttgagg
     5101 gtgggtatac ctttgaagcg ggaactttta gcggcagcga agggacgctg aatacctggt
     5161 acgtccagcc acaggtacaa atcacctgga tgggtgtgaa agattctgac catacccgga
     5221 aagacggaac acgcattgaa acggaaggcg acggaaacgt gcaaacgcga cttggggtga
     5281 aaacctacct gaacagccat caccagcgtg acgatggtaa acagcgtgag ttccagcctt
     5341 acattgaagc gaactggatc aacaacagca aagtctacgc agtgaagatg aatggtcaaa
     5401 ccgtaagccg tgatggtgcg cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag
     5461 taaataacaa ccttagcctg tgggggaatg tcggtgtgca actaggtgat aaaggctata
     5521 gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa gccgcatgtc
     5581 gagatggcat gcggcttaag ccttcctttt atccgcgatc gcggatatcg cagcgtttat
     5641 cccgtacagc ggataagatg tgtttcctga ttgactttat ccccactaaa ggataaaact
     5701 gataatatcc ccttgagcgg ataaatatgc tgtggacgta tgacatgatg agctttcaga
     5761 agatctatag cccaacgcaa ttagcgaatg caatgaaact ggttcgtcag caaaatggct
     5821 ggacgcagag cgagctggcg aaaaaaatcg gcattaagca ggcgacaatt tccaatttcg
     5881 aaaacaaccc tgacaatacc acgctgacga cattttttaa gattttacag tcgcttgaac
     5941 tctcaatgac gctatgcgac acgaaaaatg cctcgccaga atcaacagaa cagcaagatc
     6001 tggagtggta atgcctaaac ttgtcacttg gatgaacaac cagcgggtag gcgagttaac
     6061 gaagttagcc aatggcgcgc acacctttaa gtatgcaccg gagtggttag ccaaccgtta
     6121 tgccagaccg ttatcacttt cgctgccatt gcagaggggg aatatcacct ctgatgccgt
     6181 atttaacttc ttcgataacc tgttacccga tagcccgatt gtacgtgacc ggatcgttaa
     6241 acgttatcat gccaaatcca gacaaccgtt tgatttattg tcagaaatag ggcgagacag
     6301 cgttggtgcc gtgacgttaa tacccgaaga tgaaaccgta acgcatccga taatggcatg
     6361 ggaaaagctt actgaagcca gacttgaaga agtattaacg gcttataaag cagatatccc
     6421 tctgggcatg attagagaag aaaatgactt tcgcatctcg gttgctggcg cgcaggagaa
     6481 gacagcgctg ctcagaatag gcaatgactg gtgcattccg aaaggaataa cgccgacgac
     6541 gcacatcatt aaattaccga ttggcgaaat caggcagccc aatgcgacgc tcgatctcag
     6601 ccaaagcgtt gataatgagt attactgtct gctgctggcg aaagaacttg ggttgaatgt
     6661 gccggacgca gaaatcatta aagcggggaa tgtgcgcgcg ttagcggtcg aacgttttga
     6721 caggcgttgg aatgctgagc gaacggtttt acttcgcttg ccacaggagg atatgtgtca
     6781 gacattcggt ttaccttcat cggtgaaata tgaatcagat ggaggcccag gcatcgcgcg
     6841 gatcatggct tttttgatgg ggtccagcga ggcgctgaaa gatcgctatg attttatgaa
     6901 gtttcaggtg ttccagtggt tgattggcgc aacggatggc catgcaaaaa acttctccgt
     6961 atttattcag gctggcggca gttatcgact cacgccattt tacgacatca tttcagcatt
     7021 tccggtattg ggtggcgcag gaatacacat cagcgatctc aaactggcaa tgggggttaa
     7081 cgcatccaaa ggcaaaaaaa cggcaatcga taaaatttat ccgcgacatt ttctggcgac
     7141 agcaaaggtg ctgaaatttc cggaagtgca gatgcatgaa atcctgagtg actttgccag
     7201 aatgattccg gcagcactgg ataacgtgaa gacttcatta ccgacagatt ttccggagaa
     7261 cgtggtgacg gcagttgaaa ccaatgtgtt gaggttgcac cgtcgcttaa gccgagaata
     7321 cggtattaag taagatatgg ggtttggccg ttgttaatga gcaggacaat catgaccgcc
     7381 aacgactgga ttctcccttg gtttatgttg gtgcgcataa tgtatattat gttaaatatg
     7441 gtgggtttgt tcttaagttc atgtgttccg ctctttcagt agcttaactg atgaatcagt
     7501 cagcagcttt tgccgtgcaa gctacgcggc ggttcgtacc agattggcca cctccatctc
     7561 ctggttcgct ttcccctccc cccggtaaac ctcacccaac gttaattaat gatgtcagta
     7621 tgtgggcgct atccttgtat gtaaaatcca ctgacgatta cgcccatctg tgaaaaaaaa
     7681 ctcacaatgc cattcgatgc atctccatac cgaacgaggc gttatcgtct ttatcgcccc
     7741 gtagtggata tcagaaaatc aatcagggcc ttcactcttt tcgttttacc cgcgtttttg
     7801 gggtaataga gatacactgg cgggtaggtt ttccagtagc tctccatcac cggaataaag
     7861 cgctccctgt ccggctgtaa ctgccagaca ggctcaaaca agcgcccaat tcccagcccg
     7921 ttacggatcc cgtctgccat aacgtcaata tcgttgctga ttaactgccc cggcatctca
     7981 acggtcaact gctcaccgcg atcgttcagg agcagcggaa ggatgcggtt gttggtgata
     8041 aaacggtaac caatcagctg gtgatgacag agatctgccg gcacttcagg cgttccatgt
     8101 cgggagagat acgctgagga tgcatataat ccttcacgaa acggtttcat taacggtcgg
     8161 gccaccaccc ctccctcaag aatatcgcca aaacggatcc ccagatcaaa acgctctttg
     8221 atgatattca cggtaccgtc gtagaccgaa atttctaact gtatacccgg atattgctga
     8281 cagaattctg ccatcgcagg cttaagaatt aacaggtagg caaaacgcga gagcgtaatt
     8341 cgcaccatcc ctgagggctc ctgatgctgg tcgcgaatgc tttcaagtga gttttccagc
     8401 gactccaccg ccgcagcggt ctgttccagc aataattgac cggtttccgt cagctctata
     8461 cggcgggtcg tacgcacaaa aagcggatga ccgatatact cttccagcag ctttagtgca
     8521 ttgctgacgg agggaggcgt aatttccagt ttccttgcgg ctgccgaaat attgccctcc
     8581 cgcgcaatgc tctggaagat gcgaatctgg ttataaagag cgttattcat aatgttttcc
     8641 taaagcaatt ctcgcctgat tattaagtac agtctaaaaa tgcttaagtc aatgaccctc
     8701 taatcttcct gaaatacgcc tcttatactt tagtctcttt ctactacgac ctgagttcag
     8761 gagactatga tgcgaatttt atgtctggat atccctgccc ctggagcatc gctggagaaa
     8821 tatgctccgc acctcaacgc tgaagcgctg cacgcctggg gattgtataa atcaggcttc
     8881 atccgcgaca tctacttccg tcaggacaga cctggcgtcg ctatttttct tgaatgtgac
     8941 tctgtcgatg aagcgatgaa cgttatggcc gaattcccgt tggcaaaagc aggcttacta
     9001 agcttcgagt gtataccgct tggctccttt attaactggg aaaatctctt cgccgatgaa
     9061 tttaaaaata aagagtgagg accgatatga aacctgcata taaaccagtg ctatgtgtcg
     9121 tttccagcca tccgattaaa ggcgcatccg gtgtaccaac cggttttttc ctggccgagc
     9181 tgactcatcc gctgaaagta ctggaagatg ccggaataaa aacaacaata gccagtattc
     9241 gcggggggca gccaccggtg gatggatttg acctcagcga ccctgttaac gcctggtact
     9301 ggaacgaaac cgattttcag cagcgcctgg caacaacacc tgcgttatcc gagctgaaag
     9361 gtgccgatta cagcgcagtt ttctttgcag gtggtcatgg gaccatgtgg gatttccgtg
     9421 ataatcagga tgcccagcgt attatccgcg aagtgtatga aagtgatggg attgtctcag
     9481 ccgtctgcca cgggcctgcg gcattggttg acgcaaaact cagtagcggc gaatacctga
     9541 taaaagggaa aaatgtggcg gcctttacca acaaggaaga ggaagaagtt cacacaacag
     9601 atgtggttcc cttcctgctt gaaaccgcac ttcgcgagca tggtgcgctt tatcatgaag
     9661 ctccaaactg gtctgaaaac gtggttaccg acgatcgcct tatcacgggg cagaatcccg
     9721 cttcagcaca tggggttggt gtggcgctgt taaatgcact ccgccggaca gcttaattaa
     9781 ttcatcgtta ctcaccttac ttggccggtt taagtcagcc acgatccggc cttttttcag
     9841 gagctgaata tgaaaacact acctccagtc gcactaggta catggtcctg gggcacaggc
     9901 tttgccggcg gcgacaccgt ctttggtaat catctttctg atactcagat ggcagaagtg
     9961 ttcactgcgg ccatgagtaa gggcctcaat ctctgggata ccgcagcagt atatggtatg
    10021 gggagttccg agactgcgct gggagcatta gtccgtcagt ttccccgcga gaacataatt
    10081 ttatccacca aattcacgcc gcagattgcg gacaagcagt ctgcgcagcc tgtcagcgat
    10141 atgcttgagg ccagccttgg gcgcctgggc gtggacgcga ttgatatcta ctggatccat
    10201 aatcccctcg acgtcgaaaa atggacacca ggactgatcc cacttttaca aagcggtaag
    10261 gtaaaacgcg taggtgtttc caaccacaat ctggcacaaa tcagacgcgc caatgaaatc
    10321 cttaacgctt ccggttattc cctcagtgca gtacaaaatc actacagcct gctctatcgc
    10381 gcttctgaag aggccgggat ccttgattat tgccgacaaa ataacatcac gttctttgct
    10441 tacatggtcc tggagcaagg cgcgctcagt ggtcgttatg attcaaatca tcccatgcct
    10501 gccgggagtg gccgggccga aagttataac gcagtactgc cgcagataga aagactaacc
    10561 gccgccatga aaaaaatggg agccgaaagg aatgccagcg tggcacagat agccatcgca
    10621 tgggctattg cgaaaggtac ccttcccctc gttggtgcga ctaaagtcca tcacgttctg
    10681 gacgccgcct gcgcttcaga catccagtta cgcgatgagg aaatcatcct gctggaacaa
    10741 ctcgccgcag agaccagagt ggatacacga ggcgcctggg aaaaaccgat ggtgtaaagc
    10801 ccgtccggaa cagactgtca tgaatgacac acacgaacgg gctgttccgg gtgattctaa
    10861 tctgtatcac ttttactgct ttgtatgaat ggtgagaata tgaaaatcat ttgcctcgaa
    10921 gaaaattatc tcgacagcga gttagggcga gcgtgtatgc ctgttgcgct tgagcaggca
    10981 cctttccttg gcgactgggg gaaaaccgtc gctgatggtc ataatcctga ccgactccct
    11041 gctttcccgc tttaataaac tgaatgtgcc actctatctt catcccgggt taccgcttaa
    11101 aagcgtacag caggcctact tcacgggctt cagcgcagag gtcaactcca ggctttctat
    11161 gtttgcctgg ggctggcatc acgaagcggg gatccatttg ttgcggctga tgttatcggg
    11221 tgcatttgat aaatatcccc acttgcaggt aatcagtggc cactggggtg agatgttacc
    11281 cttctggctc cagcgtcttg atgacagcct gccactg
//
LOCUS       DARNVM010000157        11035 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.157, whole genome shotgun sequence.
ACCESSION   DARNVM010000157 DARNVM010000000
VERSION     DARNVM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.157"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            104..1246
                     /locus_tag="SI621_003824"
     CDS             104..1246
                     /locus_tag="SI621_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEI4066482.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1253..2017)
                     /gene="glcC"
                     /locus_tag="SI621_003825"
     CDS             complement(1253..2017)
                     /gene="glcC"
                     /locus_tag="SI621_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HEI4066483.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAACDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2268..3767
                     /gene="glcD"
                     /locus_tag="SI621_003826"
     CDS             2268..3767
                     /gene="glcD"
                     /locus_tag="SI621_003826"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HEI4066484.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLHVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARAQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3767..4819
                     /gene="glcE"
                     /locus_tag="SI621_003827"
     CDS             3767..4819
                     /gene="glcE"
                     /locus_tag="SI621_003827"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEI4066485.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPCDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4830..6053
                     /gene="glcF"
                     /locus_tag="SI621_003828"
     CDS             4830..6053
                     /gene="glcF"
                     /locus_tag="SI621_003828"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEI4066486.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPHPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6058..6462
                     /locus_tag="SI621_003829"
     CDS             6058..6462
                     /locus_tag="SI621_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HEI4066487.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6484..8655
                     /gene="glcB"
                     /locus_tag="SI621_003830"
     CDS             6484..8655
                     /gene="glcB"
                     /locus_tag="SI621_003830"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEI4066488.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDRVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGNAAAPSCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9010..10692
                     /gene="glcA"
                     /locus_tag="SI621_003831"
     CDS             9010..10692
                     /gene="glcA"
                     /locus_tag="SI621_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HEI4066489.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKLDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2566 a         2767 c         3083 g         2619 t
ORIGIN      
        1 actatttatt cgtcgtttgt attcattggg ttgattatta aggtgccgtt cgttgctgtt
       61 gcaacctgtt tgtcaccgtt aattatttgt tacccacaga actatggaat gcgatctgct
      121 gatgattaaa attgaaaaag taattaataa aaatgacctt aaggcattta tcgcttttcc
      181 gtcatcactt tatcccgacg atccaaactg gataccccct ttattcattg aacgcaacga
      241 gcatttgtct gcgaaaaatc cggggacgga ccatatcatc tggcaggcgt gggtggcaaa
      301 aaaagcaggg cagatagttg ggcgtattac cgcgcaaatc gataccttgc accgcgagcg
      361 ttacggcaaa gataccggtc atttcggcat gattgacgcc attgatgatc cgcaggtttt
      421 tgccgcgttg tttggcgcag cggaagcgtg gttgaagtca caaggtgcaa gtaagatcag
      481 cggtcctttc agcctgaata tcaatcagga aagcggatta ctgattgaag gtttcgatac
      541 accaccctgt gcgatgatgc cacacggcaa accgtggtat gccgcgcata ttgaacaatt
      601 gggttatcac aaaggcattg atttactggc gtggtggatg cagcgaaccg atctcacttt
      661 ctctccggcc ctaaaaaaac tgatggatca ggtgcgcaaa aaagtgacca ttcgctgcat
      721 caatcgccag cggtttgccg aagagatgca aatcctgcgt gagattttca actctggctg
      781 gcagcacaac tggggatttg tgccgtttac cgaacatgag ttcgcgacca tgggcgatca
      841 acttaaatat ctggtgccgg acgatatgat ctacatcgct gagattgatt ctgcaccctg
      901 cgcgtttatt gtcggcttgc cgaacatcaa cgaggcgatt gccgatctga acggatcgct
      961 ctttcccttc ggctgggcaa aattgctgtg gcgtttgaaa gtcagcggtg tgcgaactgc
     1021 gcgagtaccg ctgatgggcg tgcgggacga gtatcagttc agccgcatcg gcccggtgat
     1081 tgcgctgtta ttgattgaag ccttacgcga tccgtttgcc cgccggaaga tcgatgcgct
     1141 ggagatgtca tggatcctcg aaaccaatac aggcatgaat aacatgctgg aacgcattgg
     1201 ggcggagccg tacaagcgtt accgattgta tgaaaagcag atttgatggc agtcacccct
     1261 gcttcatctc cagcggcacc gccgagcgaa tcaaatggtg gccttccagc tcgatatcgt
     1321 ggagattctt tttcacggtc cgcacatgat cgcatgctgc gcgctgggcg acgtgcggca
     1381 gccgctgcaa caccgcgttg tagatccgcg catgctggcg atcgatctgc tttttttgct
     1441 gtggtcgatg gtagagatta tttacgctgg caaacactga attaaacatc agatcggtca
     1501 atgattgcag cgtaaacacc aacacctgat tgtgagaagc ctgacaaatg gcgagatgga
     1561 aagcgtgatc caactgcgca tgttcgatca gcgaaatctc tttgttgttc tcactggcgg
     1621 cgagcatttt ttcataacag cgggttatca caacaaaatc agcctgggtt cccagcgttg
     1681 ccgcaagcct tgccgattca ccctccagca atgcgcgaac gtcgagcaga tcgtacagcg
     1741 ttcgcggctg cgtgctgaac agatgaatta gcgggctggt atcctgcacc cgattaagcc
     1801 gtgcgacacg agaatcgcga ccctgcgccg tttcaataat cccgcgcccg cgcagcacgg
     1861 tcagcccttc acgcagtgcg gagcgtgaga agccgagctt ttcacacagt cgacgttccg
     1921 agggaagcgg ctgaccgacc ttcagtacgc cgtcgataat taaccgttcg atactctctg
     1981 caaccacttc gcaaataggg cgacgttcat ctttcattcc cggacctcgt gcacaggtag
     2041 gaccaatttt tctgttcttg actcgcaaaa cacgcacatc acgtaagtga ttatgttaca
     2101 tcaatttaac attaagttaa ccaagacaag gtcacagagc tggaaaaaaa atggtctgac
     2161 cggtagctaa agagatagac gaaaacgaaa agcccgctta ataactgttc acagaagcag
     2221 cgcgcaaaaa tcagctgcca cacaacacaa caaagcgaag cctactcatg agcatcttgt
     2281 acgaagagcg tcttgatggc gctttacccg atgtcgaccg cacatcggta ctgatggcac
     2341 tgcgtgagca tgtccctgga ctggagatcc tgcataccga tgaggagatc attccttacg
     2401 agtgtgacgg gttgagcgcg tatcgcacgc gtccattact ggttgttctg cctaagcaaa
     2461 tggaacaggt gacagcgatt ctggctgttt gtcaccgcct gcatgtaccg gtggtgaccc
     2521 gtggtgcagg caccgggctt tctggtggcg cgctgccgct ggaaaaaggt gtgttgttgg
     2581 tgatggcgcg ctttaaagag atcctcgaca ttaaccccgt tggtcgccgc gcgcgcgcgc
     2641 agccgggcgt gcgtaacctg gcgatctccc aggccgttgc gccgcataat ctctattacg
     2701 cgccggatcc ttcctcacaa atcgcctgtt caattggcgg caatgtggcg gaaaatgccg
     2761 gtggcgtcca ctgcctgaaa tatggtctga ccgtacataa cctgctgaaa attgaagtgc
     2821 aaacgctgga cggcgaggcg ctgacgctgg gatcggacgc gctggattcg cctggttttg
     2881 acctgctggc gctgttcacc ggatcggaag gtatgctcgg cgtgaccacc gaagtgacgg
     2941 taaaactgct gccgaagccg cccgtggcgc gggttctgtt agccagcttt gactcggtag
     3001 aaaaagccgg acttgcggtt ggtgacatca tcgccaatgg cattatcccc ggcgggctgg
     3061 agatgatgga taacctgtcg atccgcgcgg cggaagattt tattcatgcc ggttatccgg
     3121 tcgacgccga agcgattttg ttatgcgagc tggacggcgt ggagtctgac gtacaggaag
     3181 actgcgagcg ggttaacgac atcttgttga aagcgggcgc gactgacgtc cgtctggcac
     3241 aggacgaagc agagcgcgta cgtttctggg ccggtcgcaa aaatgcgttc cctgcggtag
     3301 gacgaatctc cccggattac tactgcatgg atggcaccat cccgcgccgc gccctgcctg
     3361 gcgtactgga aggcattgcc cgtttatcgc agcaatatga tttacgcgtt gccaacgtct
     3421 ttcatgccgg agacggcaac atgcacccgt taatcctttt cgatgccaac gaacccggtg
     3481 aatttgcccg cgcggaagag ctgggcggga agatcctcga actctgcgtt gaagttggcg
     3541 gcagcatcag tggggaacat ggcatcgggc gcgaaaaaat caatcaaatg tgcgcccagt
     3601 tcaacagcga tgaaatcacg accttccatg cggtcaaggc ggcgtttgac cccgatggtt
     3661 tgctgaaccc tgggaaaaac attcccacgc tacaccgctg tgctgaattt ggtgccatgc
     3721 atgtgcatca cggtcattta cctttccctg aactggagcg tttctgatgc tacgcgagtg
     3781 tgattacagc caggcgctgc tggagcaggt gaatcaggcg attagcgata aaacgccgct
     3841 ggtgattcag ggcagcaata gcaaagcctt tttaggtcgt cctgtcaccg ggcaaacgct
     3901 ggatgtgcgt tgtcatcgcg gcattgttaa ttacgacccg accgagctgg tgataaccgc
     3961 tcgtgccgga acgccgctgg tggcgattga agcggcgctg gagagcgccg ggcaaatgct
     4021 cccctgcgag ccgccgcatt atggtgaaga agccacctgg ggcgggatgg tcgcctgcgg
     4081 gctggcgggg ccgcgtcgcc cgtggagcgg ttcggtccgc gattttgtcc tcggcacgcg
     4141 catcattacc ggcactggaa aacatctgcg ttttggtggc gaagtgatga aaaacgttgc
     4201 cggatacgat ctctcacggt taatggccgg aagctacggt tgtctgggtg tgctcactga
     4261 aatctcgatg aaagtgttac cgcgaccacg cgccaccctg agtctgcgtc gggaaatcag
     4321 cctgcaagaa gccatgaatg aaatcgccca gtggcaactc cagccattac ccattagtgg
     4381 cttatgttac ttcgacaatg cgttgtggat ccgccttgag ggcggcgaag gatcggtaaa
     4441 agcagcgcgt gaactgctgg ggggcgaaga ggttgcgggt cagttctggc agcaattgcg
     4501 tgaacaacaa ctgccgttct tctcgttacc aggtacctta tggcgcattt ccttaccctg
     4561 cgatgcgccg atgatggatt tacccggcga gcaactgatc gactggggcg gggcgttacg
     4621 ctggctgaaa tcgacagccg aggacaatca aatccatcgc atcgcccgca acgctggcgg
     4681 tcatgcgacc cgctttagtg ccggagatgg tggctttgcc ccgctatcgg ctcctttatt
     4741 ccgctatcac cagcagctta aacagcagct cgacccttgc ggcgtgttta atcccggtcg
     4801 catgtacgcg gagctttaag gagcaggcta tgcaaaccca attaaccgaa gagatgcggc
     4861 agaacgcgcg ggcgctggaa gccgacagca tcctgcgcgc ctgtgttcac tgcggatttt
     4921 gtaccgcaac ctgcccaacc tatcagctcc tgggcgatga actggacggg ccgcgcgggc
     4981 gcatctatct gattaaacag gtgctggaag gtaacgaagt cacgcttaaa acacaggagc
     5041 atctcgatcg ctgtctcact tgccgcaatt gtgaaaccac ctgtccttct ggtgtgcgct
     5101 atcacaattt gctggatatc gggcgtgata ttgtcgagca gaaagtgaaa cgcccactgc
     5161 cggagcgaat gctgcgcgaa ggattgcgcc aggtagtgcc gcatccggct gtcttccgtg
     5221 cgctgacgca ggtagggctg gtgctgcgac cgtttttacc ggaacaggtc agagcaaaac
     5281 tgcctgctga aaccgtaaaa gctaaaccgc gtccaccgct gcgccataag cgtcgggttt
     5341 taatgctgga aggctgcgcc cagcctacgc tttcgcccaa caccaacgcg gcaactgcgc
     5401 gagtgctgga tcgtctgggg atcagcgtga cgccagctaa tgaagcaggc tgttgtggcg
     5461 cggtggacta tcatcttaat gcgcaagaga aagggctggc gcgggcgcgc aataatattg
     5521 atgcctggtg gcccgcgatt gaagcaggtg ccgaggctat tttgcagact gccagcggct
     5581 gcggcgcgtt tgtcaaagag tatgggcaga tgctgaaaaa cgatgcgttg tatgccgata
     5641 aagcgcgtca ggtcagtgag ctggcggtcg atttagtcga acttctgcgc gaggaaccgc
     5701 tggaaaaact ggcaattcgc ggcgataaaa aactggcctt ccactgcccg tgtaccctgc
     5761 aacatgcgca aaagctgaac ggcgaagtgg aaaaagtgtt acttcgtctt ggatttacct
     5821 taacggacgt tcccgacagc catctgtgct gcggttcagc gggaacatat gcgttaacgc
     5881 atcccgatct ggcgcgccag ctgcgggata acaaaatgaa tgcgctggaa agcggcaaac
     5941 cggaaatgat cgtcaccgcc aacattggtt gccagacgca tctggcgagc gccggtcgta
     6001 cctctgtgcg tcactggatt gaaattgtag aacaagccct tgaaaaggaa taacaaaatg
     6061 aaaactaaag tcattcttag ccagcaaatg gcgagtgcaa ttattgccgc aggtcaggaa
     6121 gaggcgcaga aaaataactg gtctgtttcc attgctgttg ccgatgacgg cggtcatctg
     6181 ctggcgttaa gtcgcatgga cgattgcgcg ccgattgcgg cttacatctc ccaggagaaa
     6241 gcgcgtaccg ccgcgctggg gcgtcgtgaa actaagggct atgaagagat ggtgaacaac
     6301 ggacgtaccg cgttcgtgac tgcgccgtta ttaacgtcgc tggaaggcgg cgtaccggtt
     6361 gttgttgatg ggcaaattat tggtgccgtg ggcgtttctg gtttaaccgg agcacaggat
     6421 gcgcaggtcg cgaaagcggc agcagcggtg ttggcgaaat aagcgaaaac gaggagagaa
     6481 acaatgagtc aaaccataac ccagggccgt ttacgcattg acgccaattt taaacgtttt
     6541 gtggatgaag aagttttgcc gggaacaggg ttggacgctg cggcgttctg gcgcaatgtt
     6601 gatgagatcg ttcacgatct ggcaccagaa aatcgtcagt tgctggcaga acgcgatcgc
     6661 attcaggcgg cgcttgatga gtggcatcgc agcaatccgg ggccggttaa agataaagcg
     6721 gcctataaat ctttcctgcg tgaactgggc tacctggtgc cacaaccgga tcgcgtgacg
     6781 gtggaaacca cgggcattga cagcgaaatc accagccagg cggggccaca gctggtggtt
     6841 ccggcaatga acgcccgcta cgcgctgaac gcggcgaacg ctcgctgggg ctcactgtac
     6901 gatgcgttat acggcagcga catcatcccg caggaagggg cgatggtcag cggctacgat
     6961 ccgcagcgcg gtgagcaggt tatcgcctgg gttcggcgtt tcctcgatga atcactaccg
     7021 ctggaaaacg gcagctatca ggatgtggtg gcgtttaagg tggttgataa acaattacgc
     7081 atccagttga aaaatggtaa agaaaccacg ttacgcaccc cggcgcagtt tgtcggttac
     7141 cgtggcaatg ccgctgcgcc gtcctgcatt ttgctgaaaa ataacggcct gcatattgag
     7201 ctgcaaatcg atgccaacgg gcggattggc aaagacgatc cggcgcatat caacgatgtt
     7261 atcgtcgaag cggccatcag taccattctc gactgcgaag attcggtcgc ggcggttgat
     7321 gcggaagata aaatcctgct gtaccgcaac ctgctgggcc tgatgcaggg gactctgcaa
     7381 gagaaaatgg aaaaaaacgg tcggcaaatc gtgcgtaaac tgaatgacga tcgtcattac
     7441 accgccgccg atggctctga aatttctctg cacggacgct cgctgctgtt tatccgcaac
     7501 gtgggtcatt tgatgaccat tcctgtgatt tgggacagcg aaggcaatga aatcccggaa
     7561 ggcattcttg atggcgtcat gactggcgcg attgccctct atgatttaaa agtgcagaaa
     7621 aactcgcgca ctggcagcgt ctatattgtg aaaccgaaaa tgcacggtcc gcaggaagtg
     7681 gcgttcgcca acaaactgtt tacccgcatt gagacaatgc tcggtatggc accgaatacc
     7741 ctgaaaatgg gcattatgga tgaagaacgt cggacctcgc tgaacttgcg tagctgtatc
     7801 gctcaggcgc gcaaccgcgt ggcgttcatc aataccggtt tcctcgaccg taccggcgat
     7861 gaaatgcatt cggtgatgga agctggcccg atgctgcgta aaaatcagat gaaatcgacg
     7921 ccttggatca aagcctacga gcgtaataac gtgctttccg gtctgttctg tgggctgcgc
     7981 ggtaaagcgc aaattggtaa aggcatgtgg gcaatgccgg acctgatggc agacatgtac
     8041 agccagaagg gcgaccaact gcgtgccggg gcaaacacag cctgggttcc gtcaccaacc
     8101 gctgctacgc tccatgcgct gcactaccac caaaccaacg tacagagcgt gcaggccaac
     8161 attgcccaga gcgagttcaa tgctgaattt gaaccgctgc tggacgatct gctgactatt
     8221 ccggtggctg aaaacgctaa ctggtcggtg gaagagatcc aacaagagct ggataacaac
     8281 gtgcagggga ttctggggta cgtggtgcgt tgggtggagc aggggattgg ttgttcaaaa
     8341 gtgccggata ttcacaacgt ggcgctgatg gaagaccgcg caacgctgcg tatctccagc
     8401 cagcatatcg ccaactggtt acgtcacggt attctgacca aagaacaggt gcaggcgtcg
     8461 ctggagaata tggcgaaagt ggttgatcag caaaacgctg gcgatccggc ttatcgtccg
     8521 atggcgggga atttcgctaa ctcgtgtgct tttaaagctg ccagcgattt aatcttcctc
     8581 ggcgtgaaac agccaaacgg ctataccgaa ccgttattac acgcctggcg tttacgcgaa
     8641 aaagaaagtc attaatagcg tcaaaaaagc cccggcaagt tttattgtcg gggctatgat
     8701 agtaactgcg gtagtacatt tatcccggaa ataaccggag cgattatcag ggtttaacaa
     8761 tattaaatac ccgtagcgca ttactagcgg gcctaaaaca acaatatgga catcttaacg
     8821 atactctgcg cctgcgtgtg cggaacagaa acccttaccc gatatacttg ttatgccaga
     8881 ggtagcaatt gcgttagcag cgtactgttg ctgtaaagta taacgcctat attttacagt
     8941 tcagtcctct tttgcttatt acaaccttaa gtatatcaag catataaaga taataagaga
     9001 ctgaacaata tggttacctg gacccaaatg tatatgccga tgggaggact ggggctatcc
     9061 gctctggtcg ccctgatccc gataatattc ttcttcgttg cactcgcggt attacgtctg
     9121 aaaggacatg tcgctggagc aataaccctt atattatcta tcctgattgc aatattcgcc
     9181 tttaaaatgc cgattgatat ggcatttgct gctgcgggct atggctttat ttatggttta
     9241 tggcctattg cgtggattat tgtcgcggcg gtattcttgt ataaattaac cgttgccagc
     9301 ggccagttcg atattatccg cagctcggtt atctccatca ccgacgatca gcgtttgcag
     9361 gtgttactga ttggtttctc ctttggcgcg ttgctggaag gggcggctgg ctttggtgcg
     9421 ccggtggcga ttaccggtgc gctgctggtg ggcctgggct tcaaaccgtt atacgcggcg
     9481 gggctgtgtc tgattgccaa cactgcgccg gtggcgtttg gtgcgttggg cgtgccgatt
     9541 ctggtcgccg gtcaggtaac gggaatcgat ccgttccaca ttggcgcaat ggcgggacgt
     9601 cagttaccgt tcctgtcggt tcttgtgccg ttctggctgg tagcaatgat ggacggctgg
     9661 aaaggggtga aagagacgtg gccagcggcg ctggttgctg ggggaagctt cgctgtcact
     9721 cagttcttta cctctaacta tattggtccg gaactgccgg atattacttc ggcgctggtg
     9781 agtatcgtct cactcgcttt attccttaaa gtctggcggc cgaaaaatac cgaaacggca
     9841 atcagcatgg gacaatccgc aggtgcgatg gtggtgaata aaccatcttc tggcggtccc
     9901 gtgccttcag aatatagtct ggggcaaatc attcgtgcgt ggtcaccgtt tttaatctta
     9961 acggtgctgg tcaccatctg gaccatgaag ccgtttaaag cgttatttgc tccgggcggc
    10021 gcgttttatt cactggtgat taatttccag atccctcatt tgcatcaaca agtgttgaaa
    10081 gcggcaccca ttgtcgccca accaacgcca atggatgcgg tgtttaaatt agaccctctc
    10141 tcggctggcg gcaccgctat ttttattgcg gcgattatct ctatcttcat cctcggcgtg
    10201 ggcatcaaga aagggattgg cgtctttgcc gaaacgctaa ttagtctgaa gtggccgata
    10261 ttgtcgattg gcatggtgct ggcgttcgcc ttcgtcacca actattccgg tatgtccacc
    10321 acgctggcgc tggtactggc aggtacaggc gtgatgttcc cgtttttctc accgtttctc
    10381 ggctggctgg gcgtattcct taccggctcg gacacctcct ctaacgccct gtttggttca
    10441 ctgcaatcga ccacggcgca gcaaatcaac gtctctgaca ctctgctggt ggcagcaaat
    10501 accagcggcg gcgtaactgg caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc
    10561 gcgacgggca tggtgggccg agaatctgaa ctgttccgct acaccgtgaa gcacagtctg
    10621 atttttgcca gcgttatcgg cattatcacg ctgttgcagg cgtatgtgtt taccgggatg
    10681 ttagtttcgt aagtcaataa tgccggatga tcgttaatat catccggcta catttacatt
    10741 attctgtctt attaattgaa aattatttcc ctctttatta tccatgcagc aataatgttt
    10801 tgcgttcaga taacctgcta aataattaag aacgtgagaa tatctatttt tttatcacgc
    10861 aaaacaagtt tgtactaatt atgttcgctt gatgagcaaa caccgtgcta tctgttgctt
    10921 ttgcaatcgc aatcgttaaa actatcaaca acttgggata gttacattgt gaataattgt
    10981 tacttttttg tttgttgaat ctttttataa ataccccgtc tgacatcgaa taggg
//
LOCUS       DARNVM010000158        11019 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.158, whole genome shotgun sequence.
ACCESSION   DARNVM010000158 DARNVM010000000
VERSION     DARNVM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.158"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..773
                     /locus_tag="SI621_003832"
     CDS             <1..773
                     /locus_tag="SI621_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4066490.1"
                     /translation="EFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMT
                     WQPTVKVIELAVNPTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIM
                     RENFCAPWAEVEYLFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEE
                     WFALSGVSSPLVNNGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVP
                     IRTGNRFIMTWQEEKSDSPHLQQFREWLLAKSVVPQAM"
     gene            complement(807..1409)
                     /gene="cobO"
                     /locus_tag="SI621_003833"
     CDS             complement(807..1409)
                     /gene="cobO"
                     /locus_tag="SI621_003833"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEI4066491.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1419..3071)
                     /locus_tag="SI621_003834"
     CDS             complement(1419..3071)
                     /locus_tag="SI621_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HEI4066492.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGNEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3179..4459)
                     /locus_tag="SI621_003835"
     CDS             complement(3179..4459)
                     /locus_tag="SI621_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEI4066493.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4620..4937)
                     /locus_tag="SI621_003836"
     CDS             complement(4620..4937)
                     /locus_tag="SI621_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEI4066494.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(4934..6304)
                     /locus_tag="SI621_003837"
     CDS             complement(4934..6304)
                     /locus_tag="SI621_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEI4066495.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALEFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDSEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6308..7549)
                     /locus_tag="SI621_003838"
     CDS             complement(6308..7549)
                     /locus_tag="SI621_003838"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HEI4066496.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7549..8994)
                     /locus_tag="SI621_003839"
     CDS             complement(7549..8994)
                     /locus_tag="SI621_003839"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HEI4066497.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAALGIKKSIEAGTSKLNGLPVVNHGVAACRRLTEALQKPLQIRHGTPDARLLAEI
                     SMSSGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEACGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9013..10401)
                     /locus_tag="SI621_003840"
     CDS             complement(9013..10401)
                     /locus_tag="SI621_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEI4066498.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVSVNFAKQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10401..10850)
                     /locus_tag="SI621_003841"
     CDS             complement(10401..10850)
                     /locus_tag="SI621_003841"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HEI4066499.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRECCIERGLGNILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFSPSHDLEDVCQLMAHDINQRHNVDTRILEEAI"
BASE COUNT         2637 a         2784 c         2745 g         2853 t
ORIGIN      
        1 aggaattttt acatactccg ttattttcac actctaaaaa acgtattttt cttaatgatg
       61 cagggaagta ttacctgggg atagtaaaag aaacattaaa taagctggaa cgcgatacca
      121 ataccattat gacctggcaa cctacagtta aggttattga acttgccgtg aatcctacgt
      181 ttagtaccca ctggttaatc ccaaatttac atgaattcac aaagttacat cccgatatta
      241 ttgttaatat tcactcttta gctaataacg gcgattttct taatcgggag tacgatgctg
      301 tcattatgcg cgaaaatttt tgcgcgccct gggcggaagt agaatatctc tttgatgagg
      361 agattttgcc ggtatgcagc ggcagtttgc ttgcaatgtc cgatcaaaaa ctgtcagtgg
      421 cagaactgct tacagagtta ccacttctgc atcaaagtac ccgtattact ggctgggaag
      481 agtggtttgc cttatctggc gttagcagtc cgctggtcaa caacggacca cgtttcgatt
      541 tgctttcaat gctgattgcc gccgtgcgct ctaatctcgg cgtggcgctg ttaccacggt
      601 tcgctattca acatgatttg gatagtggcg atatggtgat tccctgtgat gtgcctattc
      661 gtaccggtaa tcgttttatt atgacgtggc aggaagagaa gtctgactcg ccgcacttgc
      721 agcaatttcg tgaatggttg ctggcgaaat cggttgtacc gcaggcgatg taaaacctgc
      781 gcctggcggg atgccaggcg cagttattac cagtcgatac ccggctgcgc ctgaatgccg
      841 ttatcaaaag catgtttgac ggggcggatt tcgctgaccg tatccgccat ttttaatatc
      901 tgactgtggc aaccgcgtcc ggtgacaatg acactttgtt gtgccgggcg gttttgcaga
      961 gaagcgatca cttcttcggt atccagataa tggtaagcca gcatataggt cagctcatca
     1021 agcaccacta agtcgtaacg cttgtcagcc agcatccgtt tgctttccga ccagacctct
     1081 ttcgcagcgt cgatatccgc ttgccggttc tgtgtctccc aggtaaaacc ggttcccata
     1141 atatgaaact caaccccgag cggctggaga aggttgtatt cgccgttgtc ccattgcccc
     1201 ttgatgtact gcgcaacccc caccgttttg ccatgaccca ccgcgcgcgt cacagtacca
     1261 aacgcggcag tggatttccc tttgccattg ccggtgaaaa caatcaaaat cccttttttc
     1321 tctatcgccg ccgcgacgcg cgtatcgacc tgttccttca atttctgctg gcgttgcaca
     1381 tgccgttcag ttgaaattcg cgcttccatt cattttcctt aatgtcccgc agggcaactg
     1441 gcgcagcggt gttgctgctc aaactgacta aagaaattat tgcctttatc atctaccagg
     1501 atgaatgcgg gcagattttc cacttccatc atccacaccg cttccatacc cagctccggg
     1561 tattccagac aacgcagact tttgacatat tcttgcgcca gtaacgcagc tgcaccgcca
     1621 atactaccca gattgaagcc accatgtttg tgacaggcat cggtgacctg ctggctgcgg
     1681 ttacctttcg ataacattac cagactgccg ccagcagcct ggaaggtatc gacataaccg
     1741 tccattcgac cgcccgtggt tggacccagt gaaccgcagg ccatgttctc cggtgttttc
     1801 gccgggccag cgtaatagac aatatgatgc ttcaggtaat ccggcatcgg ttcgccgcta
     1861 tccagccgcg cttttatttt ggcgtgggcg atatcgcgcg ccactacgat gggaccactt
     1921 aacgacacgc gggtacctac cggtaatctg gcgagatcct gcatcacgtc acgcaacgga
     1981 cggtttagat ccagttgtac atgttgcgcg tgattttctt cccgcagtga ggctggaata
     2041 tattgccccg gattgtgttc gagtttttcc agccagatac cgtgcttgtt aatttttgct
     2101 ttgatattgc gatcggcaga acaggagagc gccatagcta tcgggcagga gccaccgtga
     2161 cgcggcagac gaatgacgcg aatatcatga gcgaaatatt tacctccaaa ttgcgcacca
     2221 ataccaaatt gctgactggc ctcgagcaat actttttcca gctcaatatc acggaatgct
     2281 tgtccttgct cgtttccgct ggtgggcaaa ttgtcgtaat atttcgtaga ggccagcttg
     2341 gcaactttta aggtttgatc ggctgaaagc cctccgacga caaaggcgat gtggtaaggc
     2401 gggcaggcag cggtgcccag cgatttcatt ttttcgatta aaaatgcggt cagtttttct
     2461 ggttgcaata aggatttggt ttcctgataa agtgcggctt tattggcgga gccgccccct
     2521 ttattaacac agagaaaatg gtactcatta ccagcgacag cgctgatatc aatctgcgcc
     2581 gggagattgg tttgcgtatt gacttcggta tacatatcca gcggcgcatt ttgtgaaaag
     2641 cgcagattat tttcctggaa agtactgtaa atgcctttgc ttaaggcttc agcgtcgtta
     2701 cctcccgtcc atatctgttg ccctttactg gcaacaatag ttgcggtgcc ggtatcctga
     2761 cagttgggta ataccccttt tgctgagact tcagcattac gtaataactg caacgcaacg
     2821 tatttatcat tactactggc ctgcggatcg ttaagaatgc tggcgacctg ttgcaaatga
     2881 gcggaacgta agaaaaaaga ggcttcataa aatgcctgcc gggcaagcag cgttaatgct
     2941 tcaggagcga tttttatcac ttcttcgccg tctaattccg ttactgtaat atgttgatcg
     3001 ctaattaatg tatattctgt accgtctttg ttttgtagaa acggttcttg ccagataaat
     3061 ggtttggaca tatttcactt ttcaccttaa acgttagttt tacttaattg ccgtctaact
     3121 tcatgatccg gcttataaaa atgcgtagtc attggcttct gattgcttaa gatccccctc
     3181 agggcagagc aacgttcgat tcattgggtt ttaatgcttt accccgacag ccctcgccga
     3241 aatgttcgcc ttcccaccga ctcaccatcg cagcgcccat tgcgttgctc atgacgtttg
     3301 tcgctgaacg ccccatatca aggaacggat caacgcccat cagcaagatc agccccgctt
     3361 ccggaatatt gaactggttg agcgtggcgg cgataaccac cattgaggcg cgtggtacgc
     3421 cagccattcc tttcgaggtc aacatcagga tcaacagcat ggtgatttgc tcaccgatgg
     3481 ataaatggat attgcaggcc tgggcgatga aaactgtggc gaaggagcag taggccattg
     3541 atccaacgag attaaatgag tagccaatgg gtaagacaaa gctggcaatt ttgggggaaa
     3601 cgccaaattg ctccagtttt tcaagcgttc ccggaaaagc cgcttcagaa ctggatgtgg
     3661 taaatgccag cagggcgggt tccgaaaggg cgcgggtcag gcgtctgatg caggggccaa
     3721 cataaacgat ggccagaccg ataagcagca cccaaagtaa taacatggtg aaataaaatt
     3781 cacccataaa gatcccggcg ctcaccataa ctgccagtcc tcgttcagca atcaatgctg
     3841 aaatagcagc gaatacggtc aggggagcga agagcatgac gtagccagtg agctttaaca
     3901 tggcatgtgc cagcgaatct aaggcgtgaa cgatggcgct gcctttctcg ccaatcgccg
     3961 tcaggctaca gccgaggaaa attgagaaca ccacgatttg caaaatttca ttgtgcgcca
     4021 tggcatcgac aatgctggtg gggaaagcat gcgaaataaa tacttttagc gtaaagggtt
     4081 ctgacgcgac cactccggtg gtttcggctc cgtgtgcaac aaaattaatt cctgtgcctg
     4141 gcatgaagaa atttactgtt atcaagccta aggcaattga cagcaatgag gcgcaaataa
     4201 ataaaaagag tgttttagaa aaaatacgac caagggcttt ggcatctccc attttagcta
     4261 tacctaccac cagggtagag accactaacg gagcgataat catttttatc agtcgtaaga
     4321 aaatcgtcgt gaatatcgat atttcttgcg catagagttt tgcggtttcc ggtgaagcag
     4381 tgttattgag cactacgccg ataatcattc caagtaccag cgccaaaaga atcatcgtgg
     4441 ttaaacttat tttcttcatt ttttcctcca tcaggtgcac cgcgataaat cagaatcagg
     4501 agaagtagat gcccgaatag agattcaggc atctacctgc tattacaacc ttacaaatag
     4561 ctgaagtgtt gaactgcaat aagtgacggg atgtcagttg gctaaaagtg ttcggatttt
     4621 tacaaatcga aactcatatt taataaaggt gcatgttgct gtgcgccata gacatcattg
     4681 tcacctacat tcccggagat aatatctcgt ggcatgacga tttttactgc gttagaagga
     4741 tcaaaccaga caatacgatg aataaagtca ggttcaacat gatataagcg cgcgatcagt
     4801 tctggggtta attgccctga tgatttaact cgctcatagt tttctttcgt tttaaataaa
     4861 atatctaaaa ccagttcgta aggtccggca tttttcgaac gaatgacttg cgccagtgag
     4921 acaattgact gtttcatgcc tgaactcctt ctggcgtcac ctgttcaata tggaaatcaa
     4981 aacgcagagc gtcgttggct tcaatcaggt gatatatgga aaattcgtat actggcccgc
     5041 cctggatatc agaaggggag aacgggaacg ccagattacc tgcggtagcg atgcggtttt
     5101 catagccgta gtgcagcatg gtagagcgca ccagcgagca aacgctgttg gcaatttcct
     5161 gggtcggtgc aactacatca aggacaatcc ccagctcatg cccggcagtt tgcatcggtt
     5221 catggtctcc catcaccccg tttttgccgt acaggtggaa attgatgcga atgctgtcat
     5281 cgtcgagcga caggttacgc gaaacgctgg ttttgacttc atcgatgatt ttatcgatcc
     5341 cggcgatcat gatcgggtcg cgcgttcctg cgatggtcag acagcggaag cccactcgtc
     5401 gcgcaccttc cagtttcagg gcatacggcg tttcttcatg cttagaaccg ctgacgtaca
     5461 cttcgctgtc attgaccgct ttaaatgtgc agcctttcag gttcaacacg ccgccaggtc
     5521 caggcaggaa gtagggatcg gatttctcat acagtgtgtg tgcagccgct gacgtttcgg
     5581 taaatttacg cttcggatta aatgtcttca gcgtaaagcc gttgtcatca atgatgccca
     5641 tcgcacagtc agagcctgag ccgggcgttg cggcgattgc cgcgcattca aggattttcc
     5701 cacaatgcag cgccagacct tcatcaaagc cttgcatgat cggaagcgca gcaaagcagg
     5761 ccgggtcgta agcgcgcccg cccagcacca cttgcgcgcc ttctttcagc gcccgctgga
     5821 agggttcgat gcccatttgc gcaacgatat acgtactttc gtcaatcgcg tcgtgggtta
     5881 acggcgggac aaactccagc gccgtgattt tgccgttatc cagcgcctgg tggacgatgg
     5941 ctttgtcaac gtctgccggg atcagcgcca ttgagaacga gaggtgctct tcctgggcaa
     6001 tctcatgaat tatttctcgg caccactcca ggtgtggtgc ggcaccagaa ccgccagccg
     6061 ttccgatcac caccggtata ttattttgca cgcctgcggt gatcatatag cgcagatcgc
     6121 gcttgacccc tgcgcgatcg gtaaacggtt tgcctgcccc gaggtagtgg gggcctggat
     6181 cggaagagcc tgcatcaacg gcgatcagat cgggcgattc tgccatcgct ttacggaagc
     6241 tctcttccgg gaatccatac ccgaggattg ccgttggcga taacacttta aaagaacgag
     6301 ccatttgtta atccttagcc tgcaacagcg cgatagtacg attcatttcg ttgaagacga
     6361 tatcgaggcc ttcgtcaaag cccatccctg gctttaccag catacgcata gggcgagcgg
     6421 caagggcgac gtggacacag gtgcgggcac tgacatcagt ttcattgcag gtgccgccct
     6481 ggtacgcttc catgctgtgg ctattgcagt aaagaaccgc atcgacgatg ttgtgaatac
     6541 tgcccagatc cggggttttg atttgcacca tgtgacaact ggcggcatca gtaaaatcaa
     6601 caatatcctg gtaggtgtta caccattcat cggccacaat tttcacgccg gaaccgaggc
     6661 gcgtcagctc tttggtaatc gcggtcagca ggcgaatttg atcgggcttg ttaccggcat
     6721 cgaccggccc ttcaatgtag agcggcaggc cttgcgcttc tttttccagg ctggcgatgt
     6781 attgcgcaca gcgaagcgga tccatatcga agatcagacc gatagtgccg tatacatcga
     6841 tgtgcagggt agggtggtag cgtgcgctgg tgcgcttgct cagaatacga tccgacagcc
     6901 agcggacata ttcgcgcagt ttttcacctt taaagcctag tttctcttcg acgttattaa
     6961 tcagtgcatg gggcagcacg tcgatgccct taaggatcat cttatcgacg gcgatatatc
     7021 gatcgtcacc gctctgacca aataatggaa tggattccgc cacgcgtggc aactgccatt
     7081 catcgcaaac tacttcagtt ttcagacggc cggttgccag cgcggtagca tcaagcagcg
     7141 cctgtgataa tccgtagcgc acggcggtat gcagcaagtt gccgtcaata cgcaatttgt
     7201 cgaagaaacg ggcattcggc aggaaagcat ccacatcgcg gcctaccagt aatggcttga
     7261 tatggtcgtt gagaaacgga ataaagtgct cagcgaggaa cagtggatcg cgaccacctg
     7321 ccccggaata ctgtacggca gcacagtcac ctacggcgac cgcgccgttt tccagaatca
     7381 actgtaccga tacgcactcc ccggcctgac gaacggcgtt aaacccctgt gttaccggct
     7441 ccccggtata aaaaaagccg tcatgacccg ctccgttttt tatcgcctgc tggtcatcga
     7501 aatagaatga ggagtagcca gcggtgaaca gggcctgttt tattttcatt atggtcttcc
     7561 tattaattta ctgtgggata cagcattgat gtcatctaca accatctgga atgtcggttg
     7621 gcgtccttca aagcgtgcgc gttcagcaac gtaatcgtgg tggaggtcga gaatatcttt
     7681 tggcaatgga acggctccag cttccagtac gcgtatcgcg ccggtgttgt cacggaccgg
     7741 aagaattttg cccgcgttac acgcggctgg tgcaaacggc acatccagta cgcccgcttc
     7801 aaatgccagc accgtaccgc gcgcaatatc accattaccc agttcgaaca ctttattgag
     7861 taccgcgcga acctcgctct taatcagttc aatttccagt tcaaccgcag ggcacggcgg
     7921 gaatttctgc tcattgacca tgttgagcat ctggcgggag gctttcagcc cctgaatgtt
     7981 ggcagcggca gtcgggattc cacaggcttc gtgcggactt ttggtgatga ctttggtggc
     8041 accagacatc cccgcgacgg ctgcgcccca ggagataata gcgaacgctt tcgattcgtc
     8101 ttccggaaag ccgcccatcc actggtgaaa cacggtactt aattcatagt cggtatagcc
     8161 atagctctga aaatattcgt gtgcaagttc acgcagagac tggatcgccg caacatcctg
     8221 cgtcaggctg ccgacctgtc cataaccaac ggtgatagat ttcacaccct gttccagcgc
     8281 cagcaagcct tcgattatcg cgatagagtg cgagataaac ggcgggatca gcgtacctgt
     8341 tagtgggcca aacggttcgc ggttgatgcg gatcccgtgt tcttcataca tccccattaa
     8401 acggtcgcag tactgccagt cgcggatcga tttttccagc gttacgcgtt tggcatacgg
     8461 aatgttgtag gaaatgccgc cgccttcata actggtaaag ccgctggaca tcgagatttc
     8521 tgccagcaga cgcgcatctg gcgtgccgtg gcggatctgt agcggttttt gcagggcttc
     8581 ggtgaggcga cggcaagctg cgacaccatg gttaactacc ggcaaaccgt tcagtttaga
     8641 ggttccggct tcgatggatt ttttaatccc taaagccgct tcttcgtacc gattcagacg
     8701 ggtatatgca tcgatagtgc tcggcagcag atcgcactct tcttgtagtg ttttcagtag
     8761 ttcgatgtgt tcgtccatca gcgccacacc tgcgcgcggt tggctaaggg ttttaccttc
     8821 cttgtctgct ttcaacagcg caagagagaa gcgtttatgc tcaggaatgg tttgttggta
     8881 tttaacgcca tcttcaaagt tttcaacatc cttgccggtt tcccaggttt tcagtacctg
     8941 ttgccgctcg gtcatgaatt cgtcatgggt taatttctta tttcgaagtt ccattgattg
     9001 cagccttaat tgttataggg ttaagcattg aatactggtg cgcgcggcgg ccagtggatc
     9061 gactctggcg acgtttgcca gcagtggtaa tagcccccgg gaatcgcggt aatattcaaa
     9121 ttcggtcggt aagagaatgc gtttaccgtc gtcgtttaac tcgcggtatt tcaaccagtg
     9181 atgcatatcg aactgactgg cacgcgagag ccaaccgccg gaacccacga ctttacgcac
     9241 ggttgtcagg tcacgtccca tctgtaaatc gacattgccg acgcaggtgc agacctcttt
     9301 tttggtgccc gcatgtcttt cggtggcata acccacacac agtccggcca ggacggtatc
     9361 gaaatatttt tcttctgcat tcgcgggtaa gtagtcggga tgagcgacta gatgacggag
     9421 gtaatgataa aaagcgtcca actgtgcggg ttgcttagca aaattcacac tgactaactc
     9481 tttcgcactt tcaccaacca ccattgccga gacgcgcatt cccagatcgc cttccaccgt
     9541 ccgttttacg aagggttccg gtacgccatg taaaacagtg tcgggcgaga gcgtgttggc
     9601 acaggcggaa tagacatcgg tggttgcacc gcccatgtcg atcagcatga actctttcca
     9661 gctgtgatcg acgttactga ttgccttaac cagttcaaag accgtccatg gtgtcggcat
     9721 tggttcttcg cctgttttat caacaatgac atccagccct ttccctttaa cgatgcgttt
     9781 aaggaatatg tcgcagatag cctggcgtgc agctaaaggg ttcggatggt caagatcggg
     9841 cagaacgttg tcgacgatgg tcaggtcttt atgcccgagg atctcctgca cctcgtcctg
     9901 aatatcccgg tttccggcat aaataatggc gcagtcgagt ttggattctg ccaacacacg
     9961 ggcgttattc agtccgtagc tttcttctcc gccatcggtg ccgccggtga ataagagaat
    10021 atcgggctgc gtttcttcca gtgcctggat atcacggcgg ttcagcttgt aggcgtaata
    10081 ttgcgcaatt ttagcacccg cagagtgagc ggttactttc gcggtttcca gggtgataga
    10141 aggcaccaat cccattgcgg caaccgccag cccacctttg gcagaggagg aatacttcaa
    10201 tgcaacctca ccactgttta acagggggag ggcatcatct acgtttaaca cctggtccag
    10261 gctggagaaa aaccccttcg ccagatgatg ggtggtggtt ggggtcagaa cgtggttaac
    10321 caacgttaac gcgtccccct cccgggtgaa gagggctgct ttggtccacg tcgaaccaat
    10381 atcgacagag acgatttgca tcagattgcc tcttcaagaa tgcgagtatc aacgttatga
    10441 cgttggttga tatcgtgggc catcagctga caaacatctt ctaagtcatg acttggggaa
    10501 aagacgcgat caaatcccat ctctttgaac ttggtttcca cgtcagcgaa gtcatgtttt
    10561 ccgaccacca ggttaccgcc cacatagagc aggatgttac ccagcccacg ctcgatgcag
    10621 cattcgcgca tgcccagaca atcgatatcg ccatgcccat agatagagga gacgacaatc
    10681 gcgtcagcac cagtttcaat ggcagcatca atatattcgt cctggctgac cattacgccc
    10741 agattaataa ctctgaagtc atggttgcta aaaacgcggt ccagaacttt attgcctact
    10801 gcatggcagt ccgcgccaat aacgccaatc acaagtgttg ctttcttcat gggtaatcct
    10861 ttgtaagaca gagagtaaaa taagttacac acgtcgtatt tatggccgct cataacggca
    10921 caggtttaat atcacaggag tgtctttatc tgaccgtaat gttgcaattg atctattttc
    10981 aaaatgtctt tcgggaaatg attgtttaaa gtgtgtttt
//
LOCUS       DARNVM010000159        10976 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.159, whole genome shotgun sequence.
ACCESSION   DARNVM010000159 DARNVM010000000
VERSION     DARNVM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.159"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            64..1278
                     /locus_tag="SI621_003842"
     CDS             64..1278
                     /locus_tag="SI621_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HEI4066500.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDANRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1345..2628
                     /locus_tag="SI621_003843"
     CDS             1345..2628
                     /locus_tag="SI621_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066501.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRSELGLSATEFGFLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRAMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2881..3798
                     /gene="oxyR"
                     /locus_tag="SI621_003844"
     CDS             2881..3798
                     /gene="oxyR"
                     /locus_tag="SI621_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HEI4066502.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPSERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3781..5181)
                     /gene="sthA"
                     /locus_tag="SI621_003845"
     CDS             complement(3781..5181)
                     /gene="sthA"
                     /locus_tag="SI621_003845"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEI4066503.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5513..6163
                     /gene="fabR"
                     /locus_tag="SI621_003846"
     CDS             5513..6163
                     /gene="fabR"
                     /locus_tag="SI621_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HEI4066504.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6163..6522
                     /locus_tag="SI621_003847"
     CDS             6163..6522
                     /locus_tag="SI621_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HEI4066505.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6562..7662)
                     /gene="trmA"
                     /locus_tag="SI621_003848"
     CDS             complement(6562..7662)
                     /gene="trmA"
                     /locus_tag="SI621_003848"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEI4066506.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7776..7966
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8031..9875
                     /gene="btuB"
                     /locus_tag="SI621_003849"
     CDS             8031..9875
                     /gene="btuB"
                     /locus_tag="SI621_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HEI4066507.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGEMIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9820..10677
                     /gene="murI"
                     /locus_tag="SI621_003850"
     CDS             9820..10677
                     /gene="murI"
                     /locus_tag="SI621_003850"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEI4066508.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIITAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         2538 a         2611 c         2941 g         2886 t
ORIGIN      
        1 tgtatggtag taggcgtaaa attaagtttg aagcgtctat ttacaacaca ggagattgca
       61 ataatgaaaa ttattgctgc agatgttttt gtcacttgcc cagggcgtaa tttcgttacg
      121 cttaaaataa ccactgaaag cggactttgt ggattgggag atgccacatt aaatggtcgc
      181 gaattgtcgg tcgcttctta tctgaaagat catctttgtc cacaattaat tggccgcgat
      241 gccaaccgta tcgaagatat ctggcaattc ttttataaag gtgcttactg gcgacgtggc
      301 cccgtcacga tgtcagccat ttctgccatt gatatggcgt tatgggatat caaagctaaa
      361 gcggcaaata tgccattgta tcaactgctt ggtggtgctt ctcgtgaagg cgtgatggtt
      421 tattgccaca ccaccgggcg tacgattgat gaagtccttg aagactatgc aaagcaccag
      481 cagatgggct ttaaggcgat tcgtgtgcag tgcggtgtac cgggtatgca aaccacttat
      541 ggcctggcga agggcaaagg actggcgtat gaaccggcaa ccaaaggcct gtggccagaa
      601 gagcagttgt ggtcttcaga aaagtatctc gattttacgc cgaagttatt cgaagcggtg
      661 cgtaataagt tcggttttaa cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc
      721 atcgaagcgg cgcgctttgg gaagagcatt gaagattacc gcctgttctg gatggaagat
      781 ccgacgcctg ccgagaatca ggaatgtttt cgtctgattc gtcagcatac cgtgacgccg
      841 attgcggtcg gtgaagtgtt taacagtatc tgggactgca agcagttaat cgaagagcag
      901 ctgattgact atattcgcac gacgattacc catgcaggcg gcattaccgg gatgcgacgg
      961 attgctgatt ttgcatcgct ttatcaggta cgtaccggtt cgcatggccc ttcagattta
     1021 tcgcctgttt gccatgcggc ggcgctgcat tttgacctct gggtgccaaa ctttggcgtt
     1081 caggagtaca tgggctattc cgaacaaatg cttgaagtct tcccgcatag ctggcgcttc
     1141 gatgaaggtt atatgcaccc aggcgatgaa ccggggctgg gtatttcttt cgatgagaaa
     1201 ctggcggcga aatatccgta tgacccggcg tatcttcccg tcgcacgact ggaagatggc
     1261 acattgtgga actggtaata acggttcgct taattatttt ctgaatagtc atcaaatcga
     1321 ttaattccgc tcaaggagct aaaaatggac gttgatgtgt caaccagtgt tgcgggtaat
     1381 aaaccgcaac ggattcgtcg tattcagaca gtgaccctgg ttttattatt tatggcgggg
     1441 atcgttaatt ttctcgaccg ttcgtcattg agcgtggcag gcgaagcgat tcgtagcgag
     1501 ttaggattat cggcgacaga gtttggtttt ttgctttctg cattttctct gtcttatggt
     1561 ttttcacaac ttccttccgg tattttgcta gaccgctttg gcccacgaat agttttaggc
     1621 gcaggcttaa tattctggtc attaatgcag gcattaacag gaatggttaa ttcttttagc
     1681 cactttattt taatgcgtat cggtctgggt attggcgaag cgccatttat gcctgcggga
     1741 gtaaagtcga tcaccgactg gtatgcacaa aaagaacgcg gcacggcgct ggggattttt
     1801 aactcatcta ccgttatcgg tcaggctatc gcgcctcctg ctcttgtgtt gatgcaactg
     1861 gcatggggct ggcgggcaat gttcgtcatc atcggcgtgg cggggattct ggttgggatc
     1921 tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc tgactgacga agaacggacg
     1981 tatctctccg cgccggttaa accgcgtcca cagctgcaat ttagcgagtg gctggcgctg
     2041 tttaagcatc ggacaacctg ggggatgatt ttaggtttct ctggcgtcaa ttataccggt
     2101 tggctctaca tcgcgtggtt acccggttat ttgcaggccg agcaaggttt cagtctggcg
     2161 aaaaccggtt gggtggcggc gattcctttc ctcgcggcgg cagtcgggat gtgggtaaac
     2221 ggtattgttg tcgatcgact ggcgaaaaaa ggctacgacc tggctaagac gcgtaaaacg
     2281 gctattgtct gcggtttgat gatgtcggca ttaggcacgt tgctggtcgt gcaatcttcc
     2341 tcgccagctc aggctgtagc gtttatctca atggcgctat tctgcgtgca tttcgcggga
     2401 acgtctgcat ggggactggt gcaggtgatg gtgtcagaaa caaaagtggc ttccatcgcc
     2461 gggattcaaa actttggcag ttttgttttt gcttcctttg ctccgatcgt aaccggttgg
     2521 gtagtggata ccacacactc gtttaatctg gcgttggtta tcgcggcctg tgtgacgttc
     2581 accggggcgt tgtgttactt ctttattgtc aaagatcgca ttgagtaacc gttactatca
     2641 ggctctcttg ttgtgggcct gtagaataaa aaaaaagcgg atcctggaga tccgcaaaag
     2701 ttcacgttgg ctttagttat tcgagttgag aaactctcga aacgggcagt gacttcaagg
     2761 gttaaaagag gtgccgctcc gtttctgtga gtaattatca gtcagaatgc ttgataggga
     2821 taatcgttcg ttgctatgct acctatcgcc gcgaactatc gtggcaatgg aggatggata
     2881 atgaatattc gtgatcttga gtacctggtg gcattggccg aacaccgcca ttttcggcgt
     2941 gcggcagatt cctgccacgt tagccagccg acgcttagcg ggcaaattcg taagctggaa
     3001 gatgagttgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt cacccaggcg
     3061 ggaatgctgc tggtggatca ggcgcgtacc gtactgcgtg aggtgaaagt ccttaaagag
     3121 atggcaagcc agcagggcga gacgatgtcc ggaccgctgc atattggttt gattcccacg
     3181 gttggaccgt acctgctacc gcatattatc ccgatgctgc accagacctt tccaaagctg
     3241 gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaag
     3301 ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccattg
     3361 tttgatgaac caatgctgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc
     3421 gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagacgg tcactgtttg
     3481 cgcgatcagg caatgggttt ctgctttgaa gccggggcgg atgaagatac acacttccgc
     3541 gcgaccagcc tggaaacact gcgcaacatg gtagcggcag gtagcgggat cactttattg
     3601 cctgcgctgg ctgtgccgtc ggagcgcaaa cgcgatgggg ttgtttatct gccgtgcatt
     3661 aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc gctgcgcagc
     3721 cgctatgagc agctggcaga ggccatccgc gcaagaatgg atggccattt cgataaagtg
     3781 ttaaaacagg cggtttaaac cgtttaacgc agctacccga taggcttccg ccatcgtcgg
     3841 gtagttaaag gtggtgttga cgaagtactc aatagtgttg ccgccacctt tctgttccat
     3901 aatcgcctga ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt gaatgcccag
     3961 aatctctttc gtttcccgat ggaacaaaat tttcagcgtg cccacgttca tgccgacgat
     4021 ttgtgcacgt gccagatgtt taaactgggc gcggcccact tcatatggca ctttcatcgc
     4081 ggtcagctgc tgttcggttt tgcccacaga gctgatttcc gggatggtgt aaatgccggt
     4141 agggatatct tcaatcagat gtgcggtggc ttcacctttc accagcgcct gcgcggcaat
     4201 gcgcccctgg tcataggccg ccgacgccag gctcggatag ccaatcacgt cgcccaccgc
     4261 gtaaacgtgc ggctgcgcgg tctgatacat gctgttgacc ttcagctgtc cgcgactgtc
     4321 agtttccagc ccaatgttct gtaacgccag cgaatcggta ttacccgtgc gaccgttggc
     4381 atagagcaga cagtcagctt tcagtttttt tcccgacttc agatgcatga tcacaccgtc
     4441 gtcacagcct tcgatcttct cgtactcttc gttgtgacga atcactacgc cactgttcca
     4501 gaagtgatag gagagagaat ctgacatctc ttgatcgaga aatgccagca ggcgatcgcg
     4561 ggtgttgatc agatccactt ttacatccat accgcggaag atcgacgcat attcacagcc
     4621 gatcactcca gcaccataga taagtacatg gcgcggttcg tggtgcatgc taagaattga
     4681 gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct gttggatgat atggacgaga
     4741 gccgcaagca ataacaaatt tttcagcggt tagtgtctcg acgctgccgt ccgggcaatc
     4801 cagcgccagc gtgtgctcgt caacaaagcg agcgtttccc tgcaatattt cacagtgatt
     4861 acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta atcacgttat cggcatggtt
     4921 aaggatatcg gcaaaagaag agcggagcag tcgcgaatgg tcgctgtaaa gtgggttttg
     4981 attgaattct ataatgcggc tgacggcgtg acggagagct ttcgacggga tggtgcccca
     5041 gtgggtgcaa ccgccgccaa cattttgata acgctcgata actgcgacgc gcgcaccttg
     5101 cttaaccagg cccattgcag cgccttcgcc gccggggccg gaacctatca ctatggcatc
     5161 gtaatcgtag gaatgtggca tggtagggct tacctgttct tatacataaa agcaacagaa
     5221 tggtaacatt ttatcgcggg taagccaatt gatccccgtc atttatctgg ctatatcgtg
     5281 agcggccttt gctttgtctg tttcttactt ttgacctgaa cgttttattg gatttttatc
     5341 gacgatactc tccgtttaag cggcaggttt ccgatgtacg taaaagaacc ggccaaagaa
     5401 ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca acactggagc aatccagtat
     5461 gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg
     5521 cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca
     5581 attaagtgct gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg
     5641 cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat
     5701 ggttgatgag agcggtttaa tgctacgcca actcatgcgc caggcgcgtc agcgtatcgc
     5761 caaaggcggg agtgtgatcc gcacctcggt ctccacattt atggagttca tcggtaataa
     5821 tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc
     5881 tgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga
     5941 aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt
     6001 cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat tagaagagcg
     6061 actggtactg caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca
     6121 agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa
     6181 gatagaggta cgctgctgct ggcgttagtt gctggcttat cgattaatgg tactttcgca
     6241 gcgctgttta gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg
     6301 gtttattgcc tgcatcaacg ttatcttaat cgcaccatgc cggtaggctt gccgggtctg
     6361 gctgctgcct gttttattct tggcgtactg ctgtacagca cggtagttcg tgcggaatat
     6421 ccggatatcg gctctaactt cttcccggca gtactgtccg tcattatggt gttctggatt
     6481 ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt aatcggtatc atgccggatg
     6541 ctggcgcacc cggcatggat tttacttcgc ggtcagtaat acgccgcact ccatatggtg
     6601 cgtgtagggg aactgatcaa acagagccag acgttcaacc ttgtgcgtct ggcttaatgt
     6661 ttccagattc ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc
     6721 ctgcaccatt ttctcggttt caccgtccag accgctgcgt ggtgggtcga caaaaatggt
     6781 ttcgcactga taactcttta aatcgatccc ttgcaggcgg ttaaactcgc gtacgccatt
     6841 catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt
     6901 agcggcaatg ttgtattgcg cggcagcgac cgacggctta gcgatttcgg tggctaatac
     6961 ccgatcaaaa ttgcgcgcca gtgctaatga aaagttaccg ttgccgcagt acagctccag
     7021 taaatcgcct tttgagcctt tggtgacgtc cagcgcccat tccagcatct gaatattcat
     7081 cgccgcgttc ggctgggtaa agctgttttc tacctgacgg tagatcatct ctttccctgc
     7141 gactggcaga cgttcatcga tgtaatcctg gtccagcgcg attttggttt tcgttgcccg
     7201 accaatcaga tgcacattca gattctgcgc tcgcagtgca tcgcgcaggg cttccgcttc
     7261 ctgacgccac tcatcatcca gcttcttatg gtacagcagg gaaaccaccg cctgattact
     7321 cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac
     7381 acccgcaatc atcgccgtca tcaactggtt gataagttca ctggcggcgg ggaagctatc
     7441 cacgcggatg cggcttttgg tttgttgatc gaaaatgatg tgatacaggt catcgccatc
     7501 gtgccagatg cggaactccg cacgcatccg gtaatgactg accggcgagc gaaacacttc
     7561 cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa
     7621 ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt
     7681 aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat gtattcctat
     7741 ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg
     7801 tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc
     7861 gatgattgcg ttatgcggac actgccatcc ggtgggaagt catcatctct tagtatctta
     7921 gatacccctc caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg
     7981 taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa
     8041 aagcttcgct gctgacggcg tgttccgtca cagccttttc tgcttgggca caggatacca
     8101 gcccggatac tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc actgtgcttg
     8161 caccaaccac cgttgtgacc cgtcaggata tcgaccgctg gcagtcgacc tcggtcaatg
     8221 atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca ggtcagctct
     8281 catctatttt tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt gatggcgtac
     8341 gcctgaatct ggcaggggtg agtggttctg ccgaccttag ccagttccct attgcgcttg
     8401 tccagcgtgt tgaatatatc cgtgggccgc gctccgccgt ttatggttcc gatgcgatag
     8461 gtggggtggt gaatatcatc acgacgcgcg atgaacccgg aacggaaatt tcagcagggt
     8521 ggggaagcaa tagttatcag aactatgatg tctccacgca gcaacaactg ggggataaga
     8581 cacgggtaac gctgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg
     8641 ggaataccgg aacgcaagcg cagacagata acgatggttt tttaagtaaa acgctttatg
     8701 gcgcgctgga gcataacttt actgatgcct ggagcggctt tgtgcgcggc tatggctatg
     8761 ataaccgtac caattatgac gcgtattatt ctcccggctc accgttgctc gatacccgta
     8821 aactctatag ccaaagttgg gacgccgggc tgcgctataa cggcgaaatg attaaatcac
     8881 aactcattac cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt
     8941 atgattcgtc cgcgacgctg gatgagatga agcagtacac cgtccagtgg gcaaataacg
     9001 ttaccgttgg ccacggtaat attggtgcgg gcgttgactg gcagaaacag actacgacgc
     9061 cgggtactgg ttatgttgag gatgggtatg atcaacgtaa cactggtatc tatctgaccg
     9121 gattgcaaca agtcggcgat tttacgtttg aaggcgcagc ccgcagcgac gataactcac
     9181 agtttggtcg tcatggaacc tggcaaacga gtgccggttg ggaattcatc gaaggctatc
     9241 gttttatcgc ttcttatggc acgtcgtaca aggcacctaa tcttgggcaa ctgtatggtt
     9301 tctacggaaa tccgaatctg aatcctgaga aaagcaaaca gtgggaaggc gcgtttgaag
     9361 gcttaaccgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgatttga
     9421 tcgattatga tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg
     9481 tcgaggcgac cgccaatttt gataccggac cactgacgca tactgtgagt tatgattatg
     9541 tcgatgcgcg caatgcgatt actgacacgc cgttgttacg ccgtgctaaa cagcaggtga
     9601 aataccagct cgactggcag ttgtatgact tcgactgggg tattacttat cagtatttag
     9661 gcactcgcta tgataaggat tactcatctt atccttatca aaccgttaaa atgggtggtg
     9721 tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc tcacctgaca gttcgtggta
     9781 aaatagccaa cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac
     9841 gggaatacac cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt
     9901 gactccggcg tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc
     9961 cattacattt atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagcgttt
    10021 attgttgagc gagtggtggc aattatcacc gcagtgcaaa aacgttatcc ccttgcgctg
    10081 gctgtggtcg cttgcaacac tgccagtacc gtttcacttc ctgcattacg cgaaaagttc
    10141 gacttcccgg ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat
    10201 ggcattgtcg gattactggc aacccgcgga acagttaaac gttcttatac tcatgagctg
    10261 atcgcgcgtt tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag
    10321 ttggctgaag cgaagctgca tggcgaagat gtttctctgg atgcgctaaa acgtatcttg
    10381 cgtccgtggt taagaatgaa agagccgcca gataccgttg tactggggtg tacccatttc
    10441 cctctactac aagaagaact gttacaggtg ctgccagagg gaacccggct ggtggattct
    10501 ggcgcagcga ttgctcgccg aacggcctgg ttgctagagc atgaagcccc ggatgcaaaa
    10561 tctgccgatg cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg
    10621 cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt agactgattt
    10681 ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac
    10741 tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg
    10801 gttctcctga gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag
    10861 cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt
    10921 tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca agacga
//
LOCUS       DARNVM010000160        10936 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.160, whole genome shotgun sequence.
ACCESSION   DARNVM010000160 DARNVM010000000
VERSION     DARNVM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.160"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(247..4149)
                     /gene="hrpA"
                     /locus_tag="SI621_003851"
     CDS             complement(247..4149)
                     /gene="hrpA"
                     /locus_tag="SI621_003851"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEI4066509.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPTLCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEDSGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQGLKDALKDKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4350..4955
                     /gene="azoR"
                     /locus_tag="SI621_003852"
     CDS             4350..4955
                     /gene="azoR"
                     /locus_tag="SI621_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEI4066510.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKSHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(5069..5929)
                     /gene="pdxI"
                     /locus_tag="SI621_003853"
     CDS             complement(5069..5929)
                     /gene="pdxI"
                     /locus_tag="SI621_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HEI4066511.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALHPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDDLARVGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(6137..>10936)
                     /locus_tag="SI621_003854"
     CDS             complement(6137..>10936)
                     /locus_tag="SI621_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEI4066512.1"
                     /translation="DSATVDNKGTMTVTDPESIGIQIDGDKAIVNNEGDSTISNGGTG
                     TQINGDDATANNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSAT
                     VDNKGTMTVTDPESMGIQIDGDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTV
                     DGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDISGDNAAVDNKGNMTVTDADSIGVL
                     INGDNATLANTGDVDVSNSATGFAITSDEGKISLAGSMQVGDFSTGLTLTGDNNSLTM
                     AMQDLNVVGQKATGINVSGDANTIDITGNILVDKDQSADNAAEYFYDASVGVNVSGHN
                     NNVTLDGKLTLVADGETTTRDVSNFDGSQENISGLIVSGDGNNINLNGGIAFVGEINA
                     LTDGSAIANKRTFFGNTPLIGIDGESKVYLNGDSTISGDLQLGYASLIQLKNKAAIEI
                     GSEATFNMRDIENYDHYYAQTPQIIKAESSSEVINNGNVDIWNISFAGIFGENTTGIN
                     NGNITLSLYDYASTNTPAPEPDNTAFLTSNGGSAVNKGVITSKVMEQHSVVNMAALTG
                     STDQRVFNNSVASMMGMEAYNKGSVLNAEDAVIDMYGRGSIGMLAIDNSTADNAGNIT
                     VDTLWIDDNDTTSLHTDLPGATAKDYGVGMATGTDTGGGARNNAIATNLEGGVITVYN
                     AGAGMAAYGNSNMVINQGIINLEKNADYDANLGSNTLVGMAVYKGATAINDQTGVINI
                     NVDTGQAFYNDGTGIILNYGEINLNGAEIDSTDSHYGAPAENLELLSELSASGENITK
                     TVIRDGFVTIKPLANYGTEILNGDVDANLWLYNEDKASLTVNGDLNIVQGLENSGSMD
                     ADKLTANASVYNRASGSMTTELLMLKGGSAFFNEGSFSGVISGDSYKQNVVNTGEMTT
                     VTDGSALINGSFVLYNEAGSTLTNSGNAIAGGENAIVNITRTSDSLSQVNRGKITATN
                     GYSAIKTASTASNSNGKWIWNTETGVINGINPDAPLIDLGRGYNFANAGTINVQGDGS
                     VAISGGTTSYTVQLVNSGTINVGTEQGKADGSNGEGLIGIKGNGSATTINNTKDGVIN
                     VYADNSWAFGGSTKAIVNNGIINLLCNIGCEIYAPNTTGTRNSQDGTADIIVPVASAT
                     PGQGNVPAAPVNAVSQQKLTNYTIGTNSDGSSGTLKANNLVISDNVKVNTGFSAGTAD
                     TTVVIDDVFKGENISGAENITSSSVVWNAKGSTDASGNVDVTMSKNAYTDVATDASVN
                     DVAKALDAGYTNNELYTSLNVGTTAELNSALKQVSGSQATTVFREARVLSNRFSMLAD
                     AAPKVGNGLAFNVVAKGDPRAELGNNTEYDMLALRKTIDLSESQTMSLEYGIARLDGD
                     GAQKAGDNGVTGGYSQFFGLKHQMSFDNGMNWNNALRYDVHNLDSSRSIAFSNTNKTA
                     DTDVKQQYLEFRSEGAKTFEPSEGLKVTPYAGVKLRHTLEGGYQERNAGDFNLNMNSG
                     SETAVDSIVGLKLDYAGKDGWSASATLEGGPNLSYAKSQRTASLAGAGSQHFNIDDGQ
                     KGGGINSLASVGVKYSSKESSLNLDAYNWKEEGISDKGVMLNFKKTF"
BASE COUNT         2522 a         2949 c         2532 g         2933 t
ORIGIN      
        1 tctggaagtt tttcatagaa agagattaaa gcctgaccga cgcaatcaag ctctattatt
       61 cgctcataat acgatctcat taaaatatca tgcttactca ttgcaatacg tcctgtttac
      121 catgggcaga gtatgaatgt aacttatgta aaatagtacc gcccttgcta tttttcagaa
      181 ttcataaaga aaaaacccgg cgctaaatgc ttaccgggtt ttagatttat caggcaaata
      241 gcagtgttaa ccgctaatct gctccatcgc ctgcaaaata cgcttatctg aaatcggata
      301 aggcgtacca agttgttgag cgaagtaact aacgcgcaac tcttctatca tccaacggat
      361 ctctttcacg tcttcatcct cacgacgtgc gggcggcagt ttgttgatcc attgctgcca
      421 cgcctgctgg acgttttcga ctttcagcat ctgagcacgg tcacgatgcg gatcaaccgc
      481 cagtttttcc agtcgttttt caatcgcctg caaataacgc agcgtgtcgc ccagccgttt
      541 aaaaccgttg ccagtgacaa aaccgcgata caccaacccg cccatctgcg ctttgatgtc
      601 agaaagcccc agcgccatag tcatatccac ccgccctttc aggcgtttgt tgatattgaa
      661 gactgcagta aggatttgct cgacctgctt cgcaatatcc accaccgtgt cgttcagttc
      721 agcgcggact ttttcatgca gcgcagcaaa gccttcttcc gtccagaccg ggccaccatt
      781 ggcgtcgatc agttgatcca caccgcagga gatacagtcg tcgatcagct ccagcacttt
      841 gccatacggg ttaaagtaca gccccagctt ggctttgttt ggcaacttct catgcaaata
      901 tttgattggc gagggaatat tcagcagcag tagacggcga agaccgttcc acattgcctg
      961 cttttgttcc agcggattat caaacagttt gatcgccacg ctgtcgcgtt catccactag
     1021 cgctggccac gccttcactt tatagttgcc acgcttctgt tcgtagcttt ccggcagctg
     1081 accaaaactc cagatatgta agccgctctg ctcgataccg tcatccgcca ccgcagacag
     1141 cgtttcctgc actttgtctt tcagcgcatc tttcagacct tgtagagagc gcccttcttt
     1201 tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt ttcaggtgat caggcacctg
     1261 atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc atccgccgta actcgcgctc
     1321 aaggctgtcg agcaacggta actccagcgg tgtgacgcgg cctaaaaacg cttcggcgta
     1381 gtttggtgca ggcacaaaat tacggcgtac cggtttcggc aacgatttaa tcagtgcgat
     1441 gatcagttca cgacgcaggc cggggatttg ccattcaaac ccgctatctt ctacctgatt
     1501 aagcagcggc agcggaatat gcacggtcac accgtccgcg tccgcgccgg gctcaaactg
     1561 atagctcaaa cgcagcttga gattgccctg atgccagaag ttcgggtaat ccagcttgct
     1621 gattttttcc gccccttctt tgatcaacat gctcttttca aagttgagca aatcaggcgt
     1681 ttcgcggctg acttttttcc accagctatc gaagtgacga gcggagatta catcgtggct
     1741 gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca accagaatat cgcggcgacg
     1801 tgatttgtgt tccagctctt ccacttcggc ccgcagtttc aggttttcac ggaagaatgc
     1861 gtgacgtgtc tgccagtcac cttccaccag agcgtggcga ataaagagtt cacgacataa
     1921 cgtcggatcg atctggctgt agttgacctt acgcgcagca acaatcggca aaccataaac
     1981 agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgcg gttcgctgta
     2041 ggtgcgttta atcaaatgct gagcaacggg ttctacccat tcagggtcga tgcgtgccgc
     2101 aatgcgcccc cacaggcggc tggtttctac cagttccgcc accattaccc atttcggcgg
     2161 ctttttaaat aagccggaac cggggaaaat ggagaaacgc gcgttacgtg cgccggtata
     2221 ttcttgttta tcggcatctt tcatgccgat atgggaaagc aaaccggtca gtaaggcgat
     2281 gtgaatttcg cgatactccg ccggttcgct gttaaccgga atgccaagtt ctttcactac
     2341 ctgacgcaac tgggtgtaga tatcctgcca ttcgcgcacg cgcagatagt tgagataatc
     2401 ggtacgacac aggcgacgga aggcgttaga agaaagcgcc ttttgctgct cgccgagata
     2461 attccacaga tttacaaacg cgaggaagtc ggattctttg tcgtggaagc ggcgatgttt
     2521 ttcgtccgat gcctgctgtt tatccatcgg acgctcgcgc ggatcctgaa tggagagcgc
     2581 ggacgtgata atcatcgcct cacgcacgca gccatgtttt tgcgcttcca gcaccatacg
     2641 cgccagacgt gggtcgacag gcaactgcga gagctggcga ccgagcggcg tcagtttata
     2701 agcgctggcc tgttcatcag tggtgatcgc gcccagttct tcgagcagac gcacgccatc
     2761 ctggatattg cgtttatccg gtgcttcgac aaacgggaac gccgcgatat cacccagccc
     2821 cagcgcggtc atctgcaaaa taaccgaggc caggttggta cgcagaatct ccggatcggt
     2881 aaactccggg cgcgagagga aatcgtcttc ggaatagaga cgaatacaga tcccttcaga
     2941 cacacgacca cagcggcctt tacgctgatt ggcagacgcc tgcgaaatcg gctcaatcgg
     3001 caaacgctgc actttggtgc gatagctgta gcggctgata cgtgctgtac cggggtcgat
     3061 aacgtattta atccccggta cggtcagcga ggtttccgcg acgttggtcg ccagcacaat
     3121 acgccgtccg ctgtgcgact ggaacacgcg gttctgttcg ctgttcgaaa gccgcgcata
     3181 aagcggcaag atttcggtat ggcgtaagtt cagcttgttc agcgcatcgg cggtatcgcg
     3241 aatttcccgc tcaccgctca taaagatcag aatgtcgccg ggactttcct ggctcagttc
     3301 gtctacggcg tcaaaaatcg cctgcaactg atcgcgctcg gtatcatcgg cttcttcaac
     3361 aatcgggcga tagcgcactt ccaccggata ggtccggccg gagacttcga taatcggcgc
     3421 attattaaag tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga taatgatttt
     3481 taggtcagga cgccgcggca gcaactcttt caaatagccg agcagaaaat cgatattcag
     3541 gctgcgttcg tgcgcttcgt caataatgat agtgtcgtac tgcatcagca ggcggtcttg
     3601 ctggatctcc gccagcagga taccgtcggt catcagcttg accatcgtgt tatcacttac
     3661 gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt tcagctcttc
     3721 cgcaatacgg ttggccaccg ttcgtgccgc cagacgacgc ggctgggtat ggccgatcag
     3781 ccctttaatc ccgcggccca gctccataca gattttcggt agctgagtcg ttttaccgga
     3841 acccgtttcc ccggcgacga tcactacctg gtgatcacga attgcttcga gaatgtcctg
     3901 ctttttctga ctaaccggca aattgtccgg ataagtaatt tccggacgtg ccgcttcacg
     3961 cagcaggact ttccctgccg cctggtcaat ctctttcgcc atctcctgga aaatggcctg
     4021 ttgtgcatca ggatttttaa ccttcttcac accgtgaaga cggcgtaaaa aacgctgtct
     4081 gtcgcgcagc atcagcgaat ccagccgctg ctgcaaggcc gtaaaggtca atttttgttg
     4141 ttctgtcata acgttagtgg gcagtaggct gcctgattaa ttttctttta aatgatagat
     4201 atagagtacc acatcgcggc tttctatgcg ttgttcaata aattcgaaca taggcttcga
     4261 tttattgcgc tatctctgtg gcaggattgt gaataaagtg tcaacaagca acagggcatc
     4321 gcccattcaa acatccataa ggaaacacca tgagcaaggt attagttctt aaatccagca
     4381 tcctggcagg gtactctcag tctaatcagt tgtccgatta ttttgttgaa caatggcgcg
     4441 aaaagcactc cgctgatgaa atcaccgtgc gtgacctggc tgcaaatccg attccggtac
     4501 tggatggcga actggttggt gcactgcgtc cgagcgatgc gccgctgact ccgcgtcagc
     4561 aggaagctct ggcgctttcc gatgagctga ttgccgagct gaaatcccac gacgttatcg
     4621 ttattgcggc accgatgtat aacttcaaca tctcgactca gttgaaaaac tattttgacc
     4681 tggttgcacg tgcaggcgtt actttccgct ataccgagaa tggtccggaa ggtctggtaa
     4741 cgggtaaaaa agccatcgtt attaccagcc gcggcgggat ccacaaagat ggaccaacgg
     4801 acctggtgac gccgtatctg tccacgttcc tcggctttat cggcattact gatgtgaaat
     4861 ttgtcttcgc cgaagggatc gcatacggtc ctgaaatggc agcgaaagcg cagtctgacg
     4921 cgaaagcagc tatcgacagt attgttgctg aataaccgga cacgatcttg atgtggactg
     4981 tagatattca gtccacatct caatccgctt acctgttttc tatcgctttg tgctcttcct
     5041 taagcacaat ctgcgagcgc ctgactcgtt attctcgcga aataccatcc aacgtagaca
     5101 acacttcctc agaaagatgc aatttttcag cagccatatt ctcccgtaaa tgcgcaacag
     5161 aagatgtccc tgggatcagc aaaatattcg gtgaacgttg taacagccac gccagcgcca
     5221 cctgcattgg tgttgcgccc aggctcgctg ctacatccga aagtgtggag gattgcagcg
     5281 gcgtaaagcc cccaagcggg aagaacggca cgtaagcaat gccaacgcgg gccaaatcat
     5341 caatcattgc gtcatcagca cgatgagcaa tgttgtattc gttctgcaca cagacgattt
     5401 cggtaatctt acgcgcctct gcgacctgcg tcggtgtgac gttgctcagg ccaatatgtt
     5461 ttaccaggcc ttgttgttgc atctctgcca gcacggtcag gctggcttca atcgatcctt
     5521 ccgccgggcc atgaccatcc cccatcatga cgcgcaggtt aaccacatcc agtacgtcca
     5581 ggccgagatt acgtagatta tcgtgtaccg ctttttgcag ctctgccgga gaaaatgcgg
     5641 gcaaccagga agcatcctca ccacgtcgtg caccaatttt agtgacaatt gtcaggtcgt
     5701 cagagtaagg atgtagcgct tcgcggataa tctgattggt gacgtgcgga ccataaaagt
     5761 cgctggtatc aatatgattg acgcccaatg ccagcgcctc gcgcagcacg gttatagcaa
     5821 cgtggcgatc tcttgggggg ccaaaaaccc cagggcctgc cagttgcatc gcgccataac
     5881 caagacggtt aacggatttt gtaccgagag taaatgtgtt gctgctcatc gcaccctgcc
     5941 ctgtaataaa acggattaag aaaaatcagt atagaaacga aaagagataa ataatgcgaa
     6001 ggcgtaaaca agattactgg atgagttgct ctattgtcct tgaagacacc aaaacaaaac
     6061 gcctaaagca tcgcatccgg caataaaaaa ccgcaacctg tatcagcatt gcggtttgtg
     6121 tatcacgtgc taaaaattag aacgttttct tgaagttcag catcacgcct ttgtcgctga
     6181 tgccttcctc tttccagttg tacgcatcca gattcagcga actttctttg ctgctgtact
     6241 tcacgccgac acttgccagg ctgttgatgc cgccgccctt ctgaccgtca tcgatgttaa
     6301 agtgctgact gcctgcacct gccaggcttg ctgtgcgctg gctcttcgcg tagctcaagt
     6361 tcggcccgcc ttccagcgta gcgctagcgc tccagccgtc tttacctgca tagtccagtt
     6421 tcagcccgac gatgctgtcc accgccgttt cgctaccact gttcatattc aggttaaagt
     6481 ctccggcatt gcgctcctga tagccacctt ccagtgtgtg acgcagtttc acacccgcat
     6541 atggtgtgac cttcagccct tcgctcggtt cgaaagtctt cgctccttcg ctgcggaact
     6601 ccaggtactg ctgtttcacg tcggtatcag cagttttgtt cgtgttgcta aatgcaatcg
     6661 aacggctgct gtcaaggttg tgaacgtcat aacgcaatgc gttgttccag ttcatgccat
     6721 tgtcgaagga catctgatgt ttcaggccaa agaactggct gtaaccgcct gtaacaccat
     6781 tatcgccagc tttctgcgca ccgtcgccat cgagacgagc gataccgtac tccagactca
     6841 tggtctgact ttcactcagg tcaatggttt tacgcagtgc cagcatgtcg tactcggtat
     6901 tgttccccag ctccgcgcgc ggatcgcctt tcgccaccac gttgaacgcc agaccgttac
     6961 ccactttcgg tgcggcatct gccagcatgc taaagcggtt gctgagcaca cgcgcttcgc
     7021 ggaataccgt ggtcgcctgg ctaccgctga cctgtttcag ggcgctgttc agttcggcag
     7081 tcgtgccgac gttcaggctg gtatacagct cgttgttggt gtaacccgca tccagtgctt
     7141 tcgcgacgtc attcaccgaa gcatcggtcg ccacatcggt ataagcgttt ttgctcatag
     7201 tgacgtcaac gttgccgctg gcatcggtac tgcctttagc gttccagaca actgaagagg
     7261 aagtgatatt ctccgcgccg ctgatatttt cacctttgaa aacatcatca atcaccacgg
     7321 tagtatcagc agttcctgca ctaaagccag tatttacctt gacgttgtca ctgataacaa
     7381 ggttattggc tttcaaggtg ccggaacttc cgtcgctatt agtaccaata gtgtaattgg
     7441 taagtttttg ctgacttaca gcattgactg gtgccgcagg aacattacct tgcccaggcg
     7501 tcgcagaagc gaccggtacg attatatccg cagttccatc ctgactgttt ctcgtccctg
     7561 tggtatttgg cgcataaatc tcgcaaccaa tattacacaa caggttgatt atcccattat
     7621 tcacaatcgc tttagtggaa ccaccaaacg cccatgaatt atctgcataa acgttaatga
     7681 caccgtcttt agtattatta atcgttgttg cagaaccatt tcctttgatg ccaatcagac
     7741 cttcaccgtt gcttccatct gctttgccct gttcagtacc cacgttaatt gtgccgctgt
     7801 tcactaactg aacagtatag ctggtggtgc ctccgctaat agcaactgaa ccatcaccct
     7861 gaacattaat tgttccggcg ttagcaaaat tatacccgcg acctaaatca atcagtggag
     7921 catctggatt gataccatta attacaccgg tttctgtatt ccaaatccat ttgccgttgc
     7981 tgttagaggc tgtgctggca gttttaatcg cactgtaacc gtttgtggcg gtaatttttc
     8041 cgcggttaac ctgtgaaaga gaatcactgg tgcgtgtgat attaacgatg gcattttccc
     8101 ctcccgcaat agcattgccg ctatttgtaa gcgttgaacc ggcttcatta taaagtacga
     8161 aactgccgtt aattaaggca ctaccatctg tgacagttgt catctccccc gtatttacaa
     8221 cgttttgttt ataactatca ccgctaatta ctccgctaaa gcttccctca ttgaagaagg
     8281 cagacccgcc ttttaacatt aacagttcgg ttgtcatact gcccgaggcg cgattgtaaa
     8341 cactggcatt ggcagtaagc ttgtcggcat ccatgctccc gctgttttcc agcccctgga
     8401 caatattcaa atcaccatta actgtcaggc tggccttatc ctcgttatat aaccataggt
     8461 tggcatcgac atcgccgttc agtatttctg taccataatt agccagcggt ttaatggtaa
     8521 caaagccatc tcggataact gtttttgtaa tgttctctcc actggcactc aattcagata
     8581 acagttccag attctctgca ggagcaccat aatgagagtc ggtgctatca atttccgctc
     8641 cgttgagatt aatctcacca tagttgagga taatgcctgt accatcatta taaaacgcct
     8701 ggcccgtatc aacattaata ttgattacac cagtttggtc gtttattgcc gtagcacctt
     8761 tatacactgc catccctacc agcgtattac tacccaggtt tgcatcgtaa tctgcatttt
     8821 tctcgaggtt aatgattccc tggttaataa ccatattact gttaccgtag gcggccatac
     8881 cggcacctgc gttatataca gtaattacac caccttccag gttggtcgca atagcgttgt
     8941 tacgagcgcc acccccggtg tctgtcccgg ttgccatacc aacgccataa tcttttgccg
     9001 ttgcccccgg aagatcggta tgcaaactgg tggtgtcatt gtcatcaatc cataacgtat
     9061 ccaccgtaat attgcctgca ttatccgccg ttgagttatc gatggctaac atgccaatgc
     9121 taccgcgtcc atacatatca atgacagcat cttcggcatt gagaacagag cctttattat
     9181 acgcctccat ccccatcatg cttgctacgc tgttattaaa cacgcgctga tccgttgacc
     9241 ccgtgagtgc agccatatta acaacgctat gttgctccat tacttttgag gtaattaccc
     9301 ctttgtttac tgcgctgccg ccgttagagg tcaagaaggc cgtgttatct ggttccggtg
     9361 ctggcgtatt agttgaggca taatcataca gagaaagtgt gatattcccg ttattgatac
     9421 ctgtggtatt ttcgccaaag ataccagcaa aactgatatt ccagatatca acattaccgt
     9481 tatttatgac ctcagagcta ctttctgcct ttataatctg tggtgtttgt gcatagtaat
     9541 gatcataatt ttcaatatcc cgcatattga aagtagcttc actgccaatc tcaatagctg
     9601 ctttattttt taactggata aggcttgcat atcctagttg cagatcacca ctgatcgttg
     9661 agtcaccatt cagatatacc ttcgattcac catcaatacc aataagtggc gtattaccga
     9721 agaaagtccg tttgttagca atggctgaac catcggtcag cgcatttatt tcaccaacaa
     9781 atgcaatccc gccattcaga ttgatgttgt taccatcacc ggaaacaata agaccagaaa
     9841 tattttcctg gctaccatca aaattcgaga catcacgtgt ggtagtttca ccatccgcaa
     9901 caagcgttaa tttaccatcc agtgtcacat tgttattatg accactgaca tttacgccca
     9961 cggaagcgtc ataaaaatac tctgccgcat tgtctgcgct ctgatcttta tcaaccagaa
    10021 tattaccagt tatatcaatt gtgttggcat caccggagac gttaatacct gtagctttct
    10081 gaccaacgac attaagatcc tgcattgcca ttgtcaggct attattatcg ccagttaatg
    10141 tcagccctgt tgaaaaatca ccgacctgca tacttcccgc gagggagatt ttcccctcgt
    10201 cgctggtaat agcaaatccc gtcgctgaat tactgacatc cacatcacca gtattggcaa
    10261 gtgtcgcgtt gtcgccattg atcagtacac cgatcgagtc tgcgtccgtg acggtcatat
    10321 tgcctttgtt atcgacggcg gcattatcgc cagaaatatc aatgccgtga ccgccgccgc
    10381 tgacatccag atcaccgtcc tggataactt ttccgttatt gccgttgatt tccgtgccgg
    10441 tggaatcctt gccgtcaacg gtagttttgc cgttgttgtt cgccgtggcg tcatcaccgt
    10501 taatctgcgt gccggtgccg ccgttggtga tggtgctctc gccttcgtta ttgacgatgg
    10561 ccttgtcgcc gtcgatctgg atccccatgg actccggatc ggtgacggtc atggtgccct
    10621 tgttatccac cgtcgcgctg tcaccggtaa tatcaatccc gtgaccgcca ccgctgacat
    10681 ccagatcgcc gtcctgaatc acttttccgt tattaccgtt gatttccgta ccggtggaat
    10741 ctttaccgtc aacggtggtt ttgccgttgt tgtttgccgt ggcgtcatca ccgttaatct
    10801 gtgtgccggt gccgccgttg ctgatggtgc tgtcgccttc gttattgacg atggccttgt
    10861 cgccgtcgat ctgaataccg atggactccg gatcggtgac agtcatggta cccttgttgt
    10921 ccaccgttgc gctgtc
//
LOCUS       DARNVM010000161        10923 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.161, whole genome shotgun sequence.
ACCESSION   DARNVM010000161 DARNVM010000000
VERSION     DARNVM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.161"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            61..822
                     /gene="adiY"
                     /locus_tag="SI621_003855"
     CDS             61..822
                     /gene="adiY"
                     /locus_tag="SI621_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HEI4066513.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRIAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            959..2296
                     /gene="adiC"
                     /locus_tag="SI621_003856"
     CDS             959..2296
                     /gene="adiC"
                     /locus_tag="SI621_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HEI4066514.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2400..4043
                     /gene="eptA"
                     /locus_tag="SI621_003857"
     CDS             2400..4043
                     /gene="eptA"
                     /locus_tag="SI621_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HEI4066515.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNETRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4040..4708
                     /gene="basR"
                     /locus_tag="SI621_003858"
     CDS             4040..4708
                     /gene="basR"
                     /locus_tag="SI621_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HEI4066516.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4709..5809
                     /gene="pmrB"
                     /locus_tag="SI621_003859"
     CDS             4709..5809
                     /gene="pmrB"
                     /locus_tag="SI621_003859"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HEI4066517.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="SI621_003860"
     CDS             complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="SI621_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HEI4066518.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5986..7488)
                     /gene="proP"
                     /locus_tag="SI621_003861"
     CDS             complement(5986..7488)
                     /gene="proP"
                     /locus_tag="SI621_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HEI4066519.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7752..8630)
                     /locus_tag="SI621_003862"
     CDS             complement(7752..8630)
                     /locus_tag="SI621_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HEI4066520.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8627..10855)
                     /gene="crfC"
                     /locus_tag="SI621_003863"
     CDS             complement(8627..10855)
                     /gene="crfC"
                     /locus_tag="SI621_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HEI4066521.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2718 a         2866 c         2694 g         2645 t
ORIGIN      
        1 tcccggtatc gctttgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt
       61 atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata
      121 agggtgaatg ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt
      181 gaaaataata ttatcgacgt ctcctctctt aataacactt tggttgctca tattagtcac
      241 gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg
      301 caacgtagcg ctacgcccat cctcactctg ccatgcctga cgccagacgt ctttcgcatt
      361 gccgcgcaac atagcatgat gcccgctgaa actgagtcag aaaaggaacg aacacgtgca
      421 ttattattca ctgtgctatc ccgttttctc gacagtaaaa aattcctttc actaatgatg
      481 tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatatccac
      541 aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag
      601 aaaaagctga aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt
      661 tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc
      721 ggctacaaca gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgcct
      781 ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag
      841 agctatatta ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc
      901 aggcgtatga tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat
      961 gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg
     1021 gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ctggcgggat
     1081 tgccatttat ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc
     1141 caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt
     1201 tgggccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa
     1261 tatcgccatg gtggtcattg gcgtaggtta tttaagttac ttcttcccga ttctgaaaga
     1321 tccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat
     1381 tgtcggtccg aaaatgatca cccgtgtgca ggccgttgcc accgtgctgg cgctgatccc
     1441 catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc
     1501 atggaacgtc agcggcctgg gcaccttcgg cgcgattcaa agtaccctta acgttacgct
     1561 gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa
     1621 acgcaacgtc cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtgct
     1681 ctccaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcctcgcc
     1741 attcggtgat gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg
     1801 cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc
     1861 gaaagccgct gccgatgacg gactgttccc accgattttt gcccgtgtaa ataaagcggg
     1921 tacgccagtg gcagggctga ttatcgtcgg tattttgatg accatcttcc agctcagtag
     1981 catttcgcca aacgcgacta aagagttcgg tctggtgtct tccgtgtcgg tcatctttac
     2041 actggtgcca tatctttaca cctgtgcggc gttactgctg ctcggccacg gtcactttgg
     2101 taaagcacgc ccggcatatc tggcagtgac taccattgcc ttcctctact gcatctgggc
     2161 cgtggtcggg tccggggcga aagaggttat gtggtcattt gtcaccctga tggtaatcac
     2221 cgccatgtat gcgctgaatt acaaccggct gcataaaaac ccgtatccct tagatgcacc
     2281 aataagcaaa gattaattcc ccttaatcaa gcaaacataa aagccaacct taagaactta
     2341 aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga
     2401 tgttgaagcg cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg
     2461 ctttttatat ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc
     2521 cgctggactc gctgcataac gtactggttt tcttgtcgat gccggtcgtc gccttcagcg
     2581 tgattaatat tgtcctgaca ctaagctctt tcttatggct taatcgaccg ctggcctgcc
     2641 tgtttattct ggtcggcgca gctgcacaat atttcataat gacttacggc atcgtcatcg
     2701 accgctcgat gatagccaat attattgata ccactccggc agaaagttat gcgttgatga
     2761 caccgcaaat gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct
     2821 ggattaaaat caaacctgcc acctcgcgcc tgcgcagtgt tcttttccgt ggagccaata
     2881 ttctggtgtc tgtactgctg attttgctgg tcgccgcact gttttataaa gactacgcct
     2941 cattgttccg caacaacaaa gagctggtga agtccttaag cccctctaac agcattgttg
     3001 ccagctggtc atggtactcc catcagcgac tggcaaatct gccgctggtg cgaattggtg
     3061 aagatgcgca ccgcaaccca ttaatgcaga acgagacacg taaaaatttg accatcctga
     3121 ttgtcggcga aacctcgcgg gcggagaact tctccctcaa tggctacccg cgtgaaacta
     3181 acccgcggct ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg
     3241 caacggcagt ttcagtaccg tgcatgttct cggatatgcc gcgagagcac tacaaagaag
     3301 agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc
     3361 tgtggaatga caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg
     3421 tcaccgcgct gaatctgcct ggtcagtgca tcaacggcga atgctatgac gaagtgctgt
     3481 tccacgggct ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca
     3541 ccatcggcag ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta
     3601 ccccaacctg cgacactaac gagatccaga cctgtaccaa agagcaactg gtgaacactt
     3661 acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag
     3721 aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt gagtcgttag
     3781 gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac
     3841 aggtgccaat gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga
     3901 actgcctgca aaaacaggcg caaacgcaac actattcaca agataattta ttctccacgc
     3961 tattgggatt aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa
     4021 cttgcaggag agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca
     4081 gggattgatt ctggcggcac aaaccgaagg ctacgcgtgc gatggcgtga caaccgcgcg
     4141 gatggcggaa caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt
     4201 acccgacgaa gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc
     4261 ggtactgatc ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg
     4321 tgccgatgac tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc
     4381 cctgctacga cgccataata atcagggcga aagtgagctg attgttggca atctgacgct
     4441 gaatatgggt cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga
     4501 atatgctctg ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct
     4561 ctacaacgac atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat
     4621 ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta
     4681 tatgctggtc gcgaatgagg aaaactaatt gaatctgatg cgttttctgc gccgaccaat
     4741 atcgctgcgc caacggctga tattgaccat tggggccatt ttgttggtgt ttgagctgat
     4801 cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct
     4861 acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag
     4921 cctgatcgtc cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt
     4981 acgccgcatc acccggccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga
     5041 caacttaacg cctattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc
     5101 gttgaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc
     5161 tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct
     5221 ggcgaaaacg catcacattg atgtagcacc gttagtggct cgacttgatc agatgatgga
     5281 gagtgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta
     5341 tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat
     5401 gctcgaccag cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca
     5461 gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta
     5521 cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc
     5581 cgttgaagat gaaggaccgg gtattgatga gagtaaatgc ggggagttga gtaaagcgtt
     5641 tgtacgtatg gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat
     5701 tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg
     5761 ggcctgggta cggctgaaga aagatcagta cgtggcaaac cagatataaa gaaagctgct
     5821 gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact
     5881 ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagacttc
     5941 aattggcctg ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg
     6001 cggatgttgc tgcaccaggc gagtacgttt cgcctgcaaa tcggcaatct cgtggtcaat
     6061 atcatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg
     6121 tatatctgac gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt
     6181 tacgccggtg attaaaccaa ccaccgccac taccatcagg taataggcag gcatcatcag
     6241 attctgcgag ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac
     6301 cgaaatatta aatgccgccg ccagcgcgct gtatcggata tgcgtcggga acatcgctgg
     6361 caaggtagaa gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc
     6421 ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac
     6481 aaacagtgca acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa
     6541 gcccatcacc ggctggacaa acagcatacc gatcataata gcgataataa tcagcacccc
     6601 gtggtcttcg gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat
     6661 gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg
     6721 tttggtggca atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc
     6781 ctgttccagt ttatcaacat gctgctggaa cgccggagtc tcttccagcg catggcgcag
     6841 gtaaagcccg ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc
     6901 gaggaagttc gcttcgccga caatggtcga aattaacacc accacgcccg cacccagcac
     6961 aaacccggca atagaaccga agtccagcca gctgcccata aatccacgtt tacggtcagg
     7021 tgagtattcc gcaacaaata tcgacgcccc ggtatattca ccaccaaccg agaaaccttg
     7081 tgccatctta cagatcaaca gcaaaatcgg tgcccaaata ccaatcgtgt cgtaggacgg
     7141 tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt
     7201 ctggcgacca tatttatcgc ccaacatacc aaagaagagt ccaccaagcg gtcgaatcag
     7261 aaagggaacg gagaaagtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg
     7321 gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat cgaaccattc
     7381 cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat
     7441 ggtgacatca cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc
     7501 agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc
     7561 gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt
     7621 aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc
     7681 taccagtgtt taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg
     7741 taactgatta tttacaccaa atttacagag aagatttcct cgaccattgc ccccgccaga
     7801 cgctgcgctg aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact
     7861 gaccagcaaa cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca
     7921 gcatgatccc cgcccgtcgg aatggtcgaa gaggcaaacc actgatggta atagtgacgc
     7981 gtggtcggtg ccgcattgat actctctgcc agatgttgtg tcgctttctc gcgcaaatca
     8041 cccagcaaca acgccaccgc ttgcgggctt cccagacggc aggcatcgcc aaatgccccc
     8101 cagcgcaact cctccagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc
     8161 ccagcacgcc agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac cagtaggcct
     8221 cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
     8281 gcaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc
     8341 agcgccagat gttgcgcgat ctgcgtcagc gccagatgac ctggatccag catcgaagcc
     8401 agtttttcca ccaatgtcag acgcaggcta agcggcgcat ttaacatccc gccgttcgtc
     8461 catgcctgaa gctgcacacg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc
     8521 tgatgggctt gtggatggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgcgga
     8581 tgaaccgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg
     8641 ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa atccagctgt
     8701 gagtcatcac agcctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt
     8761 gacgcagagc caggctttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact
     8821 cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
     8881 tagccatttg ttggcaataa agatcgactt ctgcgcttaa tgctcgttgc aacacactgt
     8941 aatcaaccgt ctctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
     9001 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcttcct
     9061 ggcgcgaaat gacttcactg aacagctggc gagtgttgaa gtggggaact gcgccgtgaa
     9121 aaacaggaaa atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgatttgtt
     9181 gctcaatggg gcgaataaca tcggttaaca cccggcaaag ggtggattcc agctcggcaa
     9241 aacgcagcgt aaagtcgcga ctaatggtgt tctgcgccgt ctgtaacagt gtctcacagc
     9301 gggtacgaat ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat
     9361 tcagcgcatc ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt
     9421 catgtttaat ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt
     9481 cgatctgatg gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa
     9541 aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgcg gcagatcgca
     9601 gagcatacaa cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga
     9661 acagcgaatc ttcccacaac tgatcggcag catgacgaat atgttcgagg tcagccagat
     9721 cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt
     9781 gctctggcgg tggtaactta ccgttgttgg ctaactcatg gcgcgcccga ttcgccaggt
     9841 agccccacat cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg
     9901 tcccggaaat cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga
     9961 acttattgac cagcacatac agcggcaccg attgccccac cgccaaaatc gcctcacgga
    10021 cctcttcatc ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg
    10081 cgcgagccag ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc
    10141 cgggggtatc cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa
    10201 actccacttc aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga
    10261 cgtccagcgc cttcgccagt cgcaccaaat cattcaaact tttcagacaa tgaaaaatag
    10321 gctgggcacc cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg
    10381 cacgcatatc tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca
    10441 ggcgctgttg taatttttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta
    10501 ccggttcctt ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat
    10561 tacgattagg caaaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt
    10621 tcatggtacc gacaatcgcc agcaccattt ccagtcggga aattttacgc aactcattat
    10681 tcagcatcgc gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg
    10741 catcgtctcc ggggacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa
    10801 gctgtaacag gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa
    10861 attcttcctt aaagcaaatt ttgttacttt tatttagcca gattgttttt gagttctgtt
    10921 ttc
//
LOCUS       DARNVM010000162        10908 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.162, whole genome shotgun sequence.
ACCESSION   DARNVM010000162 DARNVM010000000
VERSION     DARNVM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.162"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            29..790
                     /locus_tag="SI621_003864"
     CDS             29..790
                     /locus_tag="SI621_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HEI4066522.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEHPVPEEQPEEIATATVTKISECDLTATIEQRGPLTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKVESVAETDGKAFPQ
                     PAGVALPVQKDATQEDIKTETVADIVQSLPSFTETRANDLILPSLHLANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRSVASEK"
     gene            806..1225
                     /locus_tag="SI621_003865"
     CDS             806..1225
                     /locus_tag="SI621_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEI4066523.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARDLVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKTSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRRSEVMQRVLMFYRGNNGATI"
     gene            1222..1521
                     /locus_tag="SI621_003866"
     CDS             1222..1521
                     /locus_tag="SI621_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HEI4066524.1"
                     /translation="MRVYIAGPMTGYKNFNREAFHEAEEKLKQKGCTVLNPAVLPDGL
                     TQAQYMDICTAMLRCVDTIYMLKGWHRSAGAKAELALAAKLGHEVVFLEATSERD"
     gene            1508..2095
                     /locus_tag="SI621_003867"
     CDS             1508..2095
                     /locus_tag="SI621_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HEI4066525.1"
                     /translation="MSEINYQALREAAEAIKIAATPQKLLAFRMKVTPQVVLALLDER
                     ERNQQYIKRRDQENEEIALTVGKLRVELEAEKSKLNEQREYYEGVIADGSKRIAELEA
                     REIKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKAIGVPVLWTNGDAEKRMTGADWLT
                     AIEMYKPTVRIVEVNVPRAAGIRIKGEEHANKTRR"
     gene            2073..2429
                     /locus_tag="SI621_003868"
     CDS             2073..2429
                     /locus_tag="SI621_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066526.1"
                     /translation="MQIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCYHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKATTE"
     gene            2522..2740
                     /locus_tag="SI621_003869"
     CDS             2522..2740
                     /locus_tag="SI621_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HEI4066527.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMIPISPLLLVWFIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2742..3107
                     /locus_tag="SI621_003870"
     CDS             2742..3107
                     /locus_tag="SI621_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEI4066528.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFAGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            3104..3484
                     /locus_tag="SI621_003871"
     CDS             3104..3484
                     /locus_tag="SI621_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066529.1"
                     /translation="MITITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VGEFYEYKPGDWYQRSVGDKAPKWVPLYAAPPAPGNQVGELTMWVKRLVSQLKKANPD
                     CKLPEKAMDYLKRNELISAEDVLR"
     gene            3775..3930
                     /gene="hokD"
                     /locus_tag="SI621_003872"
     CDS             3775..3930
                     /gene="hokD"
                     /locus_tag="SI621_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HEI4066530.1"
                     /translation="MKQQKAMLIALIVICLTVIVTVLVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            4180..4353
                     /locus_tag="SI621_003873"
     CDS             4180..4353
                     /locus_tag="SI621_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066531.1"
                     /translation="MKIEELREIFSENGLYAVRVENGKVVYTTLIPDDHVILSIEAFI
                     EYLERLGFKVVRE"
     gene            4413..5012
                     /locus_tag="SI621_003874"
     CDS             4413..5012
                     /locus_tag="SI621_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HEI4066532.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPNGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNAVATRRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            5012..5302
                     /locus_tag="SI621_003875"
     CDS             5012..5302
                     /locus_tag="SI621_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HEI4066533.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            5299..5853
                     /locus_tag="SI621_003876"
     CDS             5299..5853
                     /locus_tag="SI621_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HEI4066534.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLVHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDSHPEWCYATCRNRIDAWL
                     KMAEFMLYLPMRDAFSSGDLKTVC"
     gene            6124..6735
                     /locus_tag="SI621_003877"
     CDS             6124..6735
                     /locus_tag="SI621_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEI4066535.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFRQKDPSAPASCQKSYDSRVLCYQRGGV
                     TVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVERLMQL"
     gene            6873..7145
                     /locus_tag="SI621_003878"
     CDS             6873..7145
                     /locus_tag="SI621_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066536.1"
                     /translation="MKFYELSSDSRSQAKITLGQLIVARANTGVDDFENLGHCIAAAF
                     IAMERHESAPEVGEGRSGNGIPVNGDQKLASNVVNVMIHYPCDSIA"
     gene            7202..7771
                     /locus_tag="SI621_003879"
     CDS             7202..7771
                     /locus_tag="SI621_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066537.1"
                     /translation="MNDLPDDYFLDADDDLVDFLEKQGEDCIGEIYRSNSVNKENGYK
                     LLSILIVGIGSSFLLLTQKTQIDFLSAGIAVFTVYWSVCAIYLVINVLTVHTYGLVST
                     SPADLYTEAYKGICESDFENLKARGFVGKNDRLSVMRRYRLKTLCVTADELLQVNETI
                     RTRLKKARIATILAPVYAIIISAITYLFF"
     gene            8116..8457
                     /locus_tag="SI621_003880"
     CDS             8116..8457
                     /locus_tag="SI621_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HEI4066538.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIIMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGAHKE"
     gene            8461..8937
                     /locus_tag="SI621_003881"
     CDS             8461..8937
                     /locus_tag="SI621_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEI4066539.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHRIGDLIARFKEAGGVVNEAEI"
     gene            8921..9313
                     /locus_tag="SI621_003882"
     CDS             8921..9313
                     /locus_tag="SI621_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HEI4066540.1"
                     /translation="MKLRYKLVIVAFFVTVIGSFIWYAGHYYSKYQHEKERADEAVRN
                     AESATAITRNVLQSLQIINTVIEANQHAKQQIALESQRIQKDIKVAVADDDCAVRIVP
                     SGAVKRLHEYANGIRVGAGGSVTSQSDG"
     gene            complement(9776..10390)
                     /locus_tag="SI621_003883"
     CDS             complement(9776..10390)
                     /locus_tag="SI621_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066541.1"
                     /translation="MRYSRQNISCLSVSAIDIPEEIMKLEDFAAYNRPQSKVSDERKF
                     LDYIHSRNRWVEFIKSIDNAKPVSIAMKNSFHSQWVESGAFIREKINDDSILLKLLTL
                     LLPTYDGDSLVLYRGENKDRFDKGLIGFCWTTDISVAEKFGRGLNAYKSPGLLLRAEA
                     PACSILAGPNAHSRYLGENEFTVNPSRLSNITVIETYPDNSFFK"
     gene            10344..10895
                     /locus_tag="SI621_003884"
     CDS             10344..10895
                     /locus_tag="SI621_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEI4066542.1"
                     /translation="MALTDKQEMFCREYLIDLNATQAAIRAGYSAKTANRTASENLSK
                     PDIQFRIAELKAQRNDLVGINAEYVLNRLIEIDQMDVLDILLQNGELKPIKDWPKVWR
                     TTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADGG
                     PVRTEITNLTPEQAAETYRKMMG"
BASE COUNT         3011 a         2357 c         2892 g         2648 t
ORIGIN      
        1 aagtgatttt taccgggagg aaattttaat ggaaaccgtt tttgatgcac tgaaagcaat
       61 gggaaaagcc acgtcggtag aactggctgc gcgacttgat atcagtcgtg aagaagtgct
      121 gaacgagctg tgggaactga aaaaggctgg attcgttgat aaaagcgtat acacctggcg
      181 tgtggccgat aacaacgttc agcaggaaca tccagtgccg gaagaacagc cggaagaaat
      241 cgccacggca acagtgacga aaatatcgga gtgcgattta accgcgacga ttgaacaacg
      301 tggtccacta actgcggatg aactggctac gttgttcggt accacatcac gcaaagtggc
      361 ttcaacgctg gcaatggcaa tcagcaaggg gcgtctgatt cgcgtaaatc agaacggtaa
      421 atttcgttac tgcataccag gtgataattt accagcagag ccgaaagttg aatcggtagc
      481 ggaaaccgat ggtaaagcct ttcctcagcc agccggtgtt gcgttaccgg tacagaaaga
      541 tgcaacacag gaagatatta aaacagaaac tgtggcggac attgtgcagt cgttgccatc
      601 gtttaccgaa acgcgtgcga atgacctgat tttaccatca ctgcatctgg caaaccgcga
      661 actgcgtcgg gcgaaaaatc atgtccagaa gtgggagcgt gtctgcgccg cgctgcggga
      721 gctgaacaag caccgggata ttgttcgaca gattactgat tcttcccgca gtgttgcatc
      781 ggaaaagtga ttaccggagg cgcttatggc aaaagtattt acacaagaag agcgggaaaa
      841 aattaaaggg caggttgttg aactcgtacg ccagagtggg cgcgagacgt tacgacaact
      901 ggaagctaaa accggggcga caagatattt aataagcgtt cttgccagag acctggttgc
      961 cagtggggat gtatacaact ctggctacgg gttattcccg tcagaacagg ctcgtaagga
     1021 ctggcaaaac gcccgcaaaa aactctcgag ggcaaagctg aagaaaacat ctgcggttga
     1081 tccggacctt atctggtcgt taccagacgg agaaatacgc cgctacgaca ggcgtcagaa
     1141 cataatctgt cgcgagtgcc ggagaagtga agttatgcag cgagttttga tgttttacag
     1201 gggtaataac ggagcaacga tatgagggtt tatatcgcag ggccgatgac cgggtataaa
     1261 aatttcaacc gtgaggcatt tcacgaggcg gaagagaagc tgaaacagaa agggtgcacg
     1321 gtgttgaatc cggcagtgct tcccgatggg ctgacacagg cgcagtacat ggatatttgt
     1381 actgcgatgc tccgctgtgt ggatacgatt tacatgctga aaggctggca tcggtcggca
     1441 ggcgctaagg cagaactggc gctggcggcg aaactggggc atgaggttgt atttctggag
     1501 gcaaccagtg agcgagatta actatcaggc tctgcgtgag gcggcagaag caataaaaat
     1561 agcagccaca ccacaaaaat tgctggcatt tcgtatgaag gtcacacctc aggttgtgct
     1621 ggcactgctg gatgaacggg aaagaaacca gcaatacata aaacgccgcg atcaggagaa
     1681 cgaggaaatt gcgctaacgg tagggaagct gcgtgttgag cttgaagcag aaaaatcaaa
     1741 actcaacgag cagcgtgagt attacgaggg agtaatcgcg gatggaagta agcgcatagc
     1801 agaactggaa gcacgggaaa taaaaccagc caaaggcgaa gttcttgtcg ttgtatctgg
     1861 ttttactggt tgcgggaaaa gcgccattgc cggggaaata gaaattgcga tgaaagctat
     1921 tggtgtgccg gttctatgga ctaatggcga tgcggaaaag cgcatgacag gagctgactg
     1981 gctgacagcg attgagatgt acaaaccaac tgtgcgcatc gtggaagtta atgtgccacg
     2041 cgccgctggc attcgcatca aaggagagga gcatgcaaat aaaaccagaa gatgagttaa
     2101 gtaatattgt tttatttccg gtaaaggagg atgacccacg taatcaggtt aattttcttt
     2161 atgagccatc ggaaaggcca tactgctatc acgcttctgt ccgggttgac gaaaaagagc
     2221 gtcaggtccg ctgtaaaatc tgcggtgcag ttgtggagcc gtttgactgg atgctctcag
     2281 tggcgaaaag agaaaccaga ctggcagatg atgtaaggct attgcgccag gaagaacagg
     2341 aaaggcggaa aaatatagaa aagttaattc agattgagcg taacgcgaaa gcgcggatac
     2401 gcagggcgac aaaagccacg actgaataat taaatttagc actataaaaa atttaatcct
     2461 taaccggagg gattccagca ctctcagaac atcaggaggc cgcctgaaag ggcggtaatg
     2521 aatggtcaca ttatttagaa aaaaatatcc gcgaaagagt agaacaacag aatttctgtt
     2581 tctcattctg tttatcgtgt tgatgatacc gatatccccg ctattactgg tctggtttat
     2641 cgggaaaata attgagcccg ttattgaatt gtataacgac gtggtatggg cgtcattcaa
     2701 cacactgcac aataaaatta atccgtataa ggaaagctga tatggcactg acgaaaaaac
     2761 aacgcgcaga actgcgcatg aagttcggtg gtcgctgtgc ttattgcggc tgtgaacttg
     2821 gcgaaaagtg gcatgcagac catgtaaaac cggtcattcg ttttgctgga aatatgcttc
     2881 accaggaacg tgacgatata tccaacatgg ttccggcatg ccacccatgc aatctgcaca
     2941 agcattgcag cagcctggaa gattatcggc gaattatcag tgatggtcgt cgtgaattcc
     3001 ttgcgtccgg gaaaggcaaa gcgctggttc gtatgggatt ggttgaaatg aaatctgacc
     3061 cggttgtgtt ctggtttgaa aaatatcaag aaggggctac ggcatgatca ctattaccaa
     3121 agagcgactg caatggctgg ctaacatttc tggccgcgat gacattgacg atatagacgg
     3181 cggtgaaatt cgtgaactgg cgcttatcgc actggcatcg ctggaggcag agccggttgg
     3241 tgagttttat gaatacaaac cgggtgactg gtatcagcgt tcggtcgggg ataaagcgcc
     3301 aaagtgggtg ccgttatatg cagctccacc agcacctggc aaccaggttg gcgaattaac
     3361 aatgtgggtt aaacgactgg tcagtcaact gaaaaaagct aatccagact gcaaattacc
     3421 ggagaaggcg atggactacc tgaagcggaa cgaactgata agcgcggagg atgttttgcg
     3481 atgacttggc ctgaagcatt cacaacggca ggaatcgcaa tggcggtggc gctggtggtg
     3541 tattcgattt gccgctgggg ataaaaacgg tttgcgggaa aaggggagtt aagtaaaatt
     3601 gctgcgggtg cttgaggctg tctgcctcgg gcatgccact gtaaggcaga cagagaaaag
     3661 ccccagttaa cattacgcgt cctgcaagac gcttaacatt aatctgaggc catatctatg
     3721 ctctacacac ataggttagc ctcttacgtg ccgaaaggca aggagaagca ggctatgaag
     3781 cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt taaccgtcat agtgacggta
     3841 ctggtaacga ggaaagacct ttgcgaggta cgaatccgaa ccggccagac ggaggtcgct
     3901 gtcttcacag cttacgaacc tgaggagtaa gagaccaggc ggggtaaacc cccgccacct
     3961 ctgatgtgtc aggcatcctc aacgcacccg cacttaaccc gcttcggcgg gttttttact
     4021 ttaacagatc aagttttagc cgattcacca attaaacaga ccttcgtttg ttgataagcg
     4081 ggtatgcctg actagaatcg atgcacttaa gtagcgcgca gggagaagag ggatggaccc
     4141 cgaacagggg agtgctattt atctggaagg attctgttga tgaaaatcga agaattgcgt
     4201 gaaattttta gtgaaaatgg cctctatgct gtgcgcgttg agaatgggaa agttgtctac
     4261 acaacgttaa tccctgatga tcatgtaatt ttatctatcg aggcattcat tgaatacctt
     4321 gaaaggctcg gtttcaaggt ggttcgcgaa tgagttataa ttcgtaagcc agcctgaaca
     4381 actggcaacc tacagcgcca ttggagacag caatggcgca tatacaactg gtcaaacaaa
     4441 cctcttccgg attacttctc ccggcgacgc cggagagttg cgattttttg catcaaatca
     4501 aaataggtga gtggatacac gcggacttta agcgagtgcg taactacgcg ttccacaagc
     4561 gttttttcaa actcctgcaa ctgggattcg attactggac tccgaacggt ggggcgatca
     4621 cgcctcgaga acgagaactg gtatcaggct tcgttgagta tctgtgcgaa tcagttggtc
     4681 gggaacacac tccagccctg agcgaagccg cagagcaata tctgaatgcc gttgcgacac
     4741 gcagaacccg ggatacggca ttgctaaagt catttgacgc tttccgcgag tgggtaacca
     4801 ttcaggccgg attttacacc gagcatattt atcctgatgg tagtcgtggg cgcagggcaa
     4861 aatctatcgc atttgcgaac atggacgaaa ccgagtttca gcaggtttat aaatctgtac
     4921 tgaatgtgct gtggaactgg atcctgttcc gtaaattttc ctctccggag gaagtcgaaa
     4981 atgtggccgc acagttactg gagtttgcgt aatggtggat ttacgtaaag cggcgcgggg
     5041 gcagatgtgc accgtcagaa ttcctggcta ctgcaatcac aatcccgaaa cttctgtgct
     5101 ggcgcattac aggctggcgg ggacgtgcgg aacagcgaca aaaccacacg atatgcaggc
     5161 agcgattgcc tgtagctcat gccacgatct aatcgacggg cgggtaaaaa ccagcgatta
     5221 caccaaagaa gaattacgcc tgatgcatgc agaaggtgtt tttcgcacac aagaaatctg
     5281 gagaaaggaa ggttatttat gatttaccca acaaatacag gcaaaagcgg ggaacacctt
     5341 cgtctcacca cgctggaaag tgtctggatt cagggaaaac tgcgcatgtg ggggcgctgg
     5401 tcgtatattg gcggcggtaa gacgggaaat atgttcaacc agttgctgac ctctaaaaag
     5461 ctgacaaaaa cggcaattaa cgaggcgctc cggaggatga aaaaagcagg tctggacaaa
     5521 cctgaacttg aggctttttt gcgggatatg atcaacggca agcaaaaaag ctggctggtg
     5581 cattgtactg atgcagaggc gttatgcatt gatcgggtga ttagtgaagt gctggcagaa
     5641 cacccaggat tgatttgtat cctccggcaa cgatatgaag ggagggggat gactaagcgc
     5701 aaaatggctg aattgctgaa tgattcacac cctgagtggt gttacgccac gtgccgtaat
     5761 cgcatagatg cgtggttgaa aatggcagag tttatgctct atctgccgat gcgtgatgca
     5821 ttctcttccg gggatctaaa aaccgtctgt tgactcaatc tgttatccgg ggctatattc
     5881 ctcacgcgcc agcaaaatct ggcgtcggga ttggcgtcct ggatagagac cgcgacagat
     5941 acacgccgcg agcgtgtttt ttattgtcgt atgcacgcgc acatctgaat tatggtgggc
     6001 tgtgtggggg cggagagatc cgcgccggtc ggtttcccgg ttacgccaac cctgcacagt
     6061 tcaccaccag atgattggcg tcgtcggtgg tgagttatta agaaaccacc agagggcgtc
     6121 attatgacaa ctcaaatttc tgttgaaact ctctccccga tcacccataa ccagattcct
     6181 gttattacca ccgaactttt ggcgcagctt tacggcactg agccggtgcg cattcgccag
     6241 aatcatcatg agaacaaagt acgcttcgtt gaagggaaac actttttcaa agttgttggt
     6301 aatgacctta aagaattgcg ggtagcttta aactactcac aaaatttgcg ggttacttta
     6361 agtaactcac aaaatttgca accatcttta agagggttac aaatttcccc gaaagcccgc
     6421 tccctcatcc tctggacaga acgcggagca gcccgtcatg ccaaaatgct cgaaaccgat
     6481 caggcgtggg atgtgttcga aaagctggaa gactgctatt tcagacaaaa ggatccgtca
     6541 gcgccagctt catgccagaa aagttacgac tcgcgagttc tctgttatca gcgaggcggt
     6601 gtcactgttt ccacaattca gttgcgggat gatgatattg ttatttccct tgagtcatgg
     6661 ctggaactgg cgagagccaa tggttggttt gttgttcgca gagataaact ggtggaaagg
     6721 ctgatgcagc tttaaaaaag ttcttgcaat tttagccata aactgcttca attccgttac
     6781 gcttcgcaaa gctgtatcgc gaggcgaacc aagcgcatga actttaccag aacccgccat
     6841 tgagcgggtt ttgttgtttc tggagaacac ccatgaaatt ttatgaatta tcttctgatt
     6901 cccgatctca ggccaaaatc acactgggtc agttgattgt ggctcgggct aacaccgggg
     6961 tggatgattt tgaaaatttg gggcattgca tcgctgccgc gtttattgcg atggagcgcc
     7021 atgaaagcgc ccctgaggtt ggtgaggggc gtagcgggaa cggaataccc gttaatggcg
     7081 atcaaaaact agcatccaac gtcgtgaacg tgatgattca ctatccgtgt gactctatag
     7141 cgtgacagcg acagtaaatt gggtaaaatt ttctacgctg caatatgcga aaggggatga
     7201 aatgaacgat ctgcccgatg actactttct tgatgctgac gatgatcttg tcgattttct
     7261 tgaaaaacag ggtgaagact gcataggtga aatctatcgg tcaaattcag taaataaaga
     7321 aaatggctat aagctgttga gcatcctaat cgtcggtatt ggatcttcat ttttgctgtt
     7381 aacgcaaaag acgcagattg actttcttag cgcaggcatt gccgttttca ccgtgtactg
     7441 gtcagtttgt gctatttatc ttgttattaa tgtgctgacg gttcacacat acggacttgt
     7501 ttccacatca cctgcagatt tgtataccga agcttataaa gggatttgcg agtccgattt
     7561 cgagaacctt aaggcaaggg gttttgtcgg taaaaatgac cgcctttcag tgatgaggcg
     7621 gtacagattg aaaacgttat gtgtgactgc tgatgagttg ctccaggtca atgaaaccat
     7681 aagaaccaga ctgaaaaaag caaggatagc gactatcctt gctcctgttt acgcgattat
     7741 tatttcagct atcacttacc tttttttctg attccttcag cggaatcacc aacgatcctt
     7801 ctaccgactg aaaaacggtc gggttgaact ggggccggtt tttgctgctg tgttggctgt
     7861 ggcttgcttt cacctgccgg cttgtttgac tggctcatct aattctccat atttgataag
     7921 gttatctttg gcgatttaac gatatcaaac acgaggatat accgccagaa gcttaatctg
     7981 gcactccatc tggccccggc atgtcccggg gctttttcgt tgttaggctc cggaaacatc
     8041 ctcgacttct tgtgagcaag cctgagggcc tgaatcttac acttagcacc atccgaactc
     8101 tcggaggtga ggcttatgaa aatgcacaat gcccctcatt cctggcctga cttactggaa
     8161 ctcttacaaa gttggtggcg tggagatacg ccgctgggcg cagtggttat gtcaattatt
     8221 atggctggtt tgcgcattgc ctattttggc ggtggcggcg gctggaaacg aaaaacgctt
     8281 gagattctgc tttgtggtgc tctgacgctg acctttgcat ccgcgcttga gtatgtcgga
     8341 tggcctaaat cactttctgt tgccattggt ggtggggtgg ggctgattgg tgttgatgct
     8401 attcgtggtg cggcaatgag ggtcatcggt aacaagttcg gtgcccataa ggagtaatta
     8461 atgcagacac tgaattccca gcgtaaagct ttccttgata tggttgcatg gtcagaagga
     8521 acggataacg gacggcagaa aaccagaaat cacggttatg atgttattgt cggtggcgaa
     8581 ctgttcactg attactccga tcacccccgc aaacttgtca cgctaaatcc gaaactcaaa
     8641 tcaacagccg ccggacgtta ccagcttctt tcacgctggt gggatgccta ccgcaagcaa
     8701 cttggcctga aagatttttc tccagaaagt caggatgctg tagcgctgca gcagattaaa
     8761 gagcgtggcg cattaccgat gattgaccgc ggcgatattc gtcaggcaat cgaccgttgc
     8821 agcaatatct gggcttcgtt gccgggcgct ggttacggtc agtatgaaca tagaatcggt
     8881 gacctgattg cccgatttaa agaagctggt ggggtggtaa atgaagctga gatataagct
     8941 ggttattgtt gccttctttg ttaccgtcat cggttctttt atctggtatg ccgggcatta
     9001 ctacagcaaa tatcaacacg aaaaggagcg cgctgatgag gctgtacgaa atgctgaatc
     9061 agcaacagcc attacccgta acgtcctgca atcactgcaa atcatcaaca cagttataga
     9121 ggctaaccag catgcaaaac agcagatcgc actggagtca cagagaatcc agaaagatat
     9181 caaagtggct gttgcggatg atgattgcgc tgttcgtatc gttccttctg gcgcagttaa
     9241 gcggttgcac gaatacgcga acggtatacg tgtcggtgcc ggtggttccg ttaccagcca
     9301 gtctgacggc tgaaacaccc cagccagatt tacccgaccc gtttacgtgg ggggcaagcc
     9361 ttaacctgaa tgttgcattg ttgtcagcgt tagcacagtg caacagggat aaggctgata
     9421 tcaggacttt tgagaaaaac agggcagcac aaaccaatgg cacgattaaa cgttgaagtt
     9481 atcccaccag acagcgaaac gatgaacggg attttttgca gtgattgaat gtaaatatgc
     9541 gcatcagccg atgacgccaa aagttatcga tgaaatgcaa cgcgaagcgg cgcgccttgt
     9601 acggcgagcg acaaacacga aggttacgtt cgttcgggac tgacattaca gaagctcctt
     9661 tgataagggg cttcgataat gtcactaaga ggaaaaattc atggcaaaac cggactggga
     9721 ggccatcgaa acggcgtacc agtggcgggt gaagtatagg gcatctattt attcatcatt
     9781 tgaaaaaaga gttgtccgga taggtttcaa taacggttat gtttgaaaga cgcgaggggt
     9841 taaccgtgaa ctcattttca ccaagataac gactgtgggc attggggcca gctaatatag
     9901 aacaggctgg agcttcagct cttaacagca aacctggtga tttgtatgca ttcaatccac
     9961 gaccaaactt ctcggcgact gaaatgtccg ttgtccagca aaatccaatg agccctttat
    10021 cgaacctgtc tttattttcc ccccgataaa gtacaaggct gtcaccatca tatgttggca
    10081 ataagagcgt taatagtttg aggagaattg agtcgtcgtt gatcttttca cgtataaagg
    10141 ccccagactc aacccattga ctatggaatg aatttttcat ggcaattgat actggctttg
    10201 cgttatcgat gcttttgata aactcaaccc atctgttccg actgtgaatg taatcaagaa
    10261 atttcctctc atcggacacc ttcgattgag ggcggttata agctgcgaaa tcttctaact
    10321 tcataatttc ctcaggtata tcaatggcac tcaccgacaa gcaagaaatg ttctgtcgcg
    10381 agtacctcat cgatttaaac gccacgcaag cggctattcg ggcggggtac agcgcaaaga
    10441 cagctaaccg taccgcatcc gaaaacctgt caaaacctga catacaattc agaatcgctg
    10501 aactgaaagc gcaacgcaat gatcttgtag gtattaatgc agaatatgta cttaatcgcc
    10561 ttattgaaat cgaccagatg gatgtgctcg acattctcct gcaaaacggt gagctaaagc
    10621 ccattaaaga ctggcctaag gtatggcgca caacgctatc aggaatggat gtcgtggaga
    10681 tggtatccgc agatagcgcc gcacttctga agaaaatcaa atggcctgat aaggttaaaa
    10741 accttgagtt gcttgggcgt catgtttctg ttcaggcgtt taaagacaac gtcaaaaatg
    10801 aagtgactgg cgctgatgga ggaccagtca gaacagaaat taccaactta acgccggagc
    10861 aggctgcaga gacgtataga aaaatgatgg gctaagtatg ccgttacc
//
LOCUS       DARNVM010000163        10886 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.163, whole genome shotgun sequence.
ACCESSION   DARNVM010000163 DARNVM010000000
VERSION     DARNVM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.163"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            64..1830
                     /gene="adeD"
                     /locus_tag="SI621_003885"
     CDS             64..1830
                     /gene="adeD"
                     /locus_tag="SI621_003885"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEI4066543.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1942..2400)
                     /gene="tnpA"
                     /locus_tag="SI621_003886"
     CDS             complement(1942..2400)
                     /gene="tnpA"
                     /locus_tag="SI621_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEI4066544.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            2832..3647
                     /locus_tag="SI621_003887"
     CDS             2832..3647
                     /locus_tag="SI621_003887"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HEI4066545.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(3644..5338)
                     /gene="yedQ"
                     /locus_tag="SI621_003888"
     CDS             complement(3644..5338)
                     /gene="yedQ"
                     /locus_tag="SI621_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HEI4066546.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(5509..5691)
                     /locus_tag="SI621_003889"
     CDS             complement(5509..5691)
                     /locus_tag="SI621_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HEI4066547.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(5770..6687)
                     /locus_tag="SI621_003890"
     CDS             complement(5770..6687)
                     /locus_tag="SI621_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEI4066548.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6860..7780
                     /gene="yedA"
                     /locus_tag="SI621_003891"
     CDS             6860..7780
                     /gene="yedA"
                     /locus_tag="SI621_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEI4066549.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFCIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(7769..8239)
                     /gene="vsr"
                     /locus_tag="SI621_003892"
     CDS             complement(7769..8239)
                     /gene="vsr"
                     /locus_tag="SI621_003892"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HEI4066550.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     DLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIN
                     RLQALGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(8220..9638)
                     /gene="dcm"
                     /locus_tag="SI621_003893"
     CDS             complement(8220..9638)
                     /gene="dcm"
                     /locus_tag="SI621_003893"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HEI4066551.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSVWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(9705..10400)
                     /locus_tag="SI621_003894"
     CDS             complement(9705..10400)
                     /locus_tag="SI621_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEI4066552.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKDHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(10440..10805)
                     /locus_tag="SI621_003895"
     CDS             complement(10440..10805)
                     /locus_tag="SI621_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEI4066553.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         2586 a         2970 c         2667 g         2663 t
ORIGIN      
        1 aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca aaatgagaaa
       61 aatatgaata attctattaa ccataaattt catcacatta gccgggcgga ataccaagaa
      121 ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa tgtctctatt
      181 ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt
      241 gctggtgttg gcgcagaata cactgatgct ccggctttgc agcggattga tgcccgcggc
      301 gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg
      361 ccggtcacct ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct
      421 catgaaatcg tcaacgtgat gggcgaagcc ggtttcgcct ggtttgcccg ctgtgccgaa
      481 caggcaaggc aaaaccaata cttgcaggtc agctcttgcg tacccgccct ggaaggctgc
      541 gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag
      601 gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg
      661 ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt
      721 ggtaaagaac ttaacgccta tattgctgcg ggtattgaaa actgccacga aagttatcag
      781 ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc
      841 gctgcccgca atctcaacgc actggcaccg ttgatcaacg aatttaacag cccgcaatgc
      901 atgctctgta ctgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc
      961 ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc
     1021 agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggtaag
     1081 caggctgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg
     1141 aaaggcgaac cgattgacgc gcaaacctta caggcggaag agtcggcgag actggcacaa
     1201 tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc
     1261 ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt
     1321 acgcactccc gctccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgtgcttt
     1381 attgccgtac ttgagcgtta cgggcaacgt ctggctccgg cttgtggttt gctcggcggc
     1441 tttggtctga atgaaggcgc gctggctgcg acggtcagcc atgacagcca taatattgtg
     1501 gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc
     1561 ggcgggctgt gcgtggtacg taacggacag gtccaaagtc atctaccgtt acccattgcc
     1621 ggcctgatga gcaccgacac ggcacagtca ctggcggagc aaattgacgc cttgaaagcc
     1681 gccgcccgtg aatgcggtcc gttaccggat gagccgttta ttcagatggc gtttctttcc
     1741 ctgccagtga tccccgcgct gaaactaacc agtcaggggc tgtttgatgg cgagaagttt
     1801 gccttcacca cgctggaagt cactgaataa taaataaata tcctccggca tagccggagg
     1861 tttttcaaat gcgcctataa ggctctctta ccagccgcgc cctaacaggc gcacacgatc
     1921 tgacatttgc atccaacttc gttacttacg gcccgtaaac gggctgcccg gatagggaat
     1981 cgataactgc tctcccattt tatcctcttc aagctggtgc tttatgtaat cctgtatctt
     2041 cgccgtgttc ttacccaccg tatcgacgta gtaccctctg caccagaact ccctgttcct
     2101 gtatttgaat ttcaaatcac caaactgctc gtaaagcatc agactgcttt tccctttcag
     2161 atatcccata aagccggata cgctcatttt gggcgggatc tccacaagca tatggatatg
     2221 atctgcacag cattcagctt ccagaatccg tacacttttc cactcacaca gctttctcaa
     2281 aatactgcct attgctctac gcttctctct gtagaacacc tgtcttcggt attttggcgc
     2341 aaaaactatg tgatatttac agttccatcg ggtgtgcgct aagctctttt cgttccccat
     2401 tgggaccccc ttttgatttc ttgtttgaca cttgcagttg ccagaccgca aggtgtttta
     2461 acaaatcaaa aggggtttta ataactggct taaagctgaa agctttccgg aacccccagc
     2521 ctagctgggg gttttctgtg cacaaaaaaa tcccgaccct gagggggtcg ggatgaaact
     2581 tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg tgttcatcac
     2641 cttatccgca atttttttcg ctgacaagaa aatattcgtg atatgaatga ttaacgctac
     2701 ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct ctggcgtcaa cgggatcgct
     2761 cccgaaaaaa tcatctttga taatttgtgc tgttttgaga atcatcctgg taaggggagt
     2821 actgcgcaac catgctttca attcaacaac cactactggt ttttagcgat cttgatggca
     2881 ccctgctgga cagtcatagt tatgactggc aaccagctgc cccctggctc agccgtttac
     2941 gcgaagcgaa tgttcccgtc attctctgta gcagtaagac atcagccgaa atgctgtact
     3001 tgcaaaaaac gttggggcta caaggtttac cgctgattgc cgaaaatggc gcagtgatcc
     3061 agcttgctga gcaatggcag gatatagacg gttttccacg catcatctca ggtattagcc
     3121 atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt aaattcacga
     3181 cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc cgtagccagg
     3241 cggcgctgac gcagctacat gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc
     3301 gtatggcaca atttaccgct cgtctgaacg aactgggctt gcagtttatg cagggtgcgc
     3361 gcttctggca cgtcctggat gcctctgccg gaaaagatca ggctgccaac tggattatcg
     3421 cgacctatca acaatcgtca ggcaaacgcc caaccacact tggcctgggc gatgggccaa
     3481 acgatgcgcc cttactggag gtaatggatt acgcggtgat tgtgaaaggg ttaaaccgtg
     3541 aaggggtgca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac
     3601 cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg
     3661 cgcatacccg attgcgcccg gcctgtttag cgagataaag ccgacggtcg gccagtgact
     3721 ggagttgttc aaaatcataa tcaccggttt cctcgctgct gcttaccccc agcgaggcac
     3781 tgatgcgtat cgtcgtactc ttggcgatca acatctcttt ttcatttagc ttcaggcgaa
     3841 tacgttctgc aacctccgca gcctgcgtca gactcgcgcc gggcagaatc acacaaaact
     3901 cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg
     3961 cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa
     4021 aatggtcaag gtcgacctga atgacagaaa aaggatgttg gtgcgtctga cacaatttag
     4081 cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt
     4141 gccacgcctg ccactgcaac gagctttgca aaacatacat gttgctaacc atccggcgaa
     4201 tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcaga gttaatgcaa
     4261 tgctgatact gccgaaatca ccacgcacgc cttcgcttag cgtatggacg cgcaccagca
     4321 caccgtcgaa atggtccaga cgttcccagc taacataacg actgttcata cgaatgccgc
     4381 cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat
     4441 tccctgttgg atggtcagga ttggaagagg tcaaaaatct cagcttactg tcatagagct
     4501 gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atagtacgca
     4561 cgggaatact catccccagc acgccatacc agtagttatt actgtctacc ggaacactga
     4621 cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg
     4681 cgcggtgacg attttctcgt tgcgaatggc cgataaacca gggttgggtg acatagccgt
     4741 aataacgcgt tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag
     4801 cacgcgagac atacatagcc tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca
     4861 agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca
     4921 ggagattccc ctcgctgact aatgcatccg atacgccatt gactggcagg gtgcgccgtc
     4981 ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat
     5041 tacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca ttacgcaaaa
     5101 agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca
     5161 gatgattacg ctggctggag atataggcat cttccagcac taccacttca cgccaggtca
     5221 gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgatatga ccaggcccca
     5281 ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc
     5341 tgacgactcc caacctgctt ttttatgatt ctggcataac ccggcgataa cgtaccgcaa
     5401 aacagcgata attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat
     5461 tggcacacca ggccggatgt gatccggcct ggtgttaatg ataccctttc aaacttcttc
     5521 cgcagaacga ctcccctccc ccggaacaag ctcggtttca tgaaaagctt cccgcttgac
     5581 cccgtacccg tcataccaac gacactcaac cataccgctg gagtatccag tgacaatcat
     5641 ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc
     5701 cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcct
     5761 gtgcgagtat taatgtgcct gaccgcgcat tttcgctacg gctttcactc ccagcaccac
     5821 gatgccgccg atgataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac
     5881 cactgcactt tgctgcccgg cgaaatgttc aatggcgtga tgcaacggcg caataccatg
     5941 taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg ataacgcttt
     6001 catcaaccag ggcgcaatga tcaataaccc tttacctaat gcctgcatca gcgcgctgga
     6061 tttttccgcc agccaatacc ccaggtcatc aatcttaacg ataaccccta ccagaccgta
     6121 gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg
     6181 cgcttctgcc acaatcccca gcgtgatggc gacgatttcc gcagacaata taaaatcggt
     6241 acgaatcgcc ccttttattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt
     6301 ctccagacgc tgctgactct gcgccggatc ttctttatgt ttacgcgcct ccagcatatg
     6361 cagcactttc tctacccctt caaagcagag aaacgcgccg ccaatcatca acagtggcgt
     6421 aatcgcccac gggataaacg cactgatgat cagcgccagc ggcaccagaa tcactttatt
     6481 gatcagcgaa cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc
     6541 tgaaacttgt tgcgcattga gcgataagtc atcccctaat acaccggcgg ttttcttcgc
     6601 cgccagtttg cccatcacgg agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt
     6661 cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt
     6721 atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacatttat ttaacaatca
     6781 agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt
     6841 atttcagccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt
     6901 tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg tggaaagctg
     6961 gcctccgtta atgatggcgg gcgttcgatt cctggcagcc ggtattttat tgctggcatt
     7021 tttgctactg cgcggacaca aacttccccc gctacgtccg ctgctcaatg ccgcgctgat
     7081 tggcctgtta ttgctggctg tcggtaatgg catggtgacg gttgccgaac atcaaaatgt
     7141 tccttccggc atcgccgccg tagtggttgc aaccgtgccc ctttttaccc tgtgcttcag
     7201 ccgcctgttt tgcattaaaa ctcgcaaact ggaatgggtg ggtatcgcca ttgggcttgc
     7261 cggaatcatc atgctcaata gcggtggaaa cttaagcggc aatccatggg gcgcgattct
     7321 gattttaatc ggctcgatta gctgggcgtt tggctcggtt tatggctcgc gcattacatt
     7381 acctgtaggg atgatggcgg gtgcgattga gatgctggcg gctggcgtgg tgttaatgat
     7441 tgcgtcgatg attgcgggtg aaaaactgac ggcgctccct tccctttcag gcttccttgc
     7501 ggtcggctat ctggcgctgt ttggttcgat tatcgccatt aacgcttata tgtatttaat
     7561 ccgcaacgtc agtccggctc tcgccaccag ctacgcctac gttaacccag tggtcgcggt
     7621 cttgctgggt acgggactgg gcggagagac gctgtcgaag attgaatggc tggcgctcgg
     7681 cgtaattgtc ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc cggcaaaacc
     7741 cgtagttgcg ccagttattc aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga
     7801 tctgcgcgct ggctccttcg ccgcagatcc actcttccag acgctcggta agcgcttcat
     7861 ccgtcagctt ctcgcgccca cgtaacgcac actcccagac aatcagtacg cgccagccga
     7921 gtgcctgcaa gcgattgata tcgcggcgat cgcgctcaac atttttacct atcttctcca
     7981 gccagaactc ggttcgggtc gcaggcactt taaacagata gcagtgatga tgatgccaga
     8041 agcagccgtg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc gggcgtccgg
     8101 ggagatccgc gtcctgaacg cgaaacgcaa ggccctgccc agttaacaaa ctggcgaggc
     8161 gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg cgggtagcct
     8221 tatcgtgaac gtcgaccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt
     8281 tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga gttaccgaac
     8341 tggcgataag cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca
     8401 aaacccatta agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg ttgctgattc
     8461 agcggatcgt caaagtcttt ttcacccgtg gccatatccc agccgcgatc gattaaaatt
     8521 tccgcgccat ctttgtaata acgcgcagac aacgtacgcg tgacgctttg cggattgttc
     8581 ggataaacca ttccataacc gaagccatta ccgcgcgcct gatgtttttt cgcataacga
     8641 tagaggtact tccacagcac cggcgtcagg atatatttcg cctcgaccat cgggtccagc
     8701 agctgggcca gcgtcactcg ctgcgcaggg aaacattcgc tgatatcacg cagggtaaaa
     8761 tcggctttca gattcagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc
     8821 ggcaggaaat gtttgccatc gatgattttc ggatcgtccg gcccgttatc ttctgcatca
     8881 gccacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt tttaccctgg
     8941 tcgtgacttt tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg
     9001 ataatgcgca ccacatcaaa gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc
     9061 cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca aggaaaaccc
     9121 gccagtaata catcgtgttc aggaatgtgt tgacgaatat gttccgccgc cgcctcatca
     9181 ctcacgcctt ctttatggct gagggtgatg tcgcggatat cttcattaaa atgatgcgtc
     9241 gccgggtcgc aataatgatt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg
     9301 gtaaacacgc actgtccgcc aatcgattca aaaccgcgac ggatgccgcc aattccggca
     9361 aatagatcga taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt gggtaacaac
     9421 gtttgcagat gggcgaactc tttctcactt aaacggtgcc ataccgagtc attcgccagc
     9481 gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag ctgcgccacc
     9541 agcgttttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg
     9601 gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg ccatctgaga
     9661 tggccgacag gttaacataa ttacagccat ttagggccac gcgctcagcc cgcgggtgaa
     9721 aacgcatcta tcaccttgtg atcgacacct tcattctccc ccgccagttc ggcactgagc
     9781 ttcgccataa actcgactaa aaattgcgcg ttgtgctgcg ccagctgctt gcctcttgcg
     9841 gtttgcatgg tttgcggtag cttcagcaac ttagtctgga aatgatccag cgcgtagcgt
     9901 ttatcatcaa gcggacgatg ctgtgcgaac ggatcttcac catcaaacag cgccacgccc
     9961 aatgcccctg aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc ttccagccga
    10021 tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc gctgaaactg
    10081 tgagcggcaa tggcatgaca aacggcctcg attttctccg ccggaaattg cacgaactct
    10141 tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg ccgttgtgga
    10201 tgatctttcg ccaggctcac aatatcgtga aaataacagg cggttaaaat caccagcata
    10261 tcgacatcgt catctgccgc cagcttctgt gccgtagccc agacgcggcg aaaatgacat
    10321 acgtcatggg ccgcgtcctg gtgctgatga tggtttttta accagttttc gaactgtgcc
    10381 tgccagtgtt gtaagtccat acgcgctccg ttgccaaaga gtcgcaacct tagcagtttt
    10441 tattcatagc gtctgctgcg tgcgggacga ggtcgggtgt accaggctat gctgcctaac
    10501 aatgccccga ccagagccat aaataaaaat ccgaccagtg cccctaacca ctttcccgcg
    10561 gtagatccta aatcgctttc aaatcccgac accggggcat cagggatccg actcatcacc
    10621 cagatgtaac gcgccattgc ccagacgaaa tagccacacc atgtatagaa tgcccacaaa
    10681 gccaatttgc cgccagggct gcgagagagt ctctcttcca tctttgtcga accgtattcc
    10741 attttgagat aacccggaaa attcagactt agaactatta tgtgataaaa atcacatttt
    10801 tccacattgg atgagccagt caagactcaa tcattttttg aacaaataga taaattttgc
    10861 gcaacggcag tgaatacagg aaaaat
//
LOCUS       DARNVM010000164        10720 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.164, whole genome shotgun sequence.
ACCESSION   DARNVM010000164 DARNVM010000000
VERSION     DARNVM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.164"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..530
                     /gene="ecpR"
                     /locus_tag="SI621_003896"
                     /gene_synonym="matA"
     CDS             <1..530
                     /gene="ecpR"
                     /locus_tag="SI621_003896"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HEI4066554.1"
                     /translation="RSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKDFVFIN
                     LNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAGLSRDI
                     RKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTVYTHRR
                     NAEAKLYSKIYKLVP"
     gene            605..1192
                     /gene="ecpA"
                     /locus_tag="SI621_003897"
                     /gene_synonym="matB"
     CDS             605..1192
                     /gene="ecpA"
                     /locus_tag="SI621_003897"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HEI4066555.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1249..1917
                     /gene="ecpB"
                     /locus_tag="SI621_003898"
                     /gene_synonym="yagY"
     CDS             1249..1917
                     /gene="ecpB"
                     /locus_tag="SI621_003898"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HEI4066556.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYRFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1943..4468
                     /gene="ecpC"
                     /locus_tag="SI621_003899"
                     /gene_synonym="yagX"
     CDS             1943..4468
                     /gene="ecpC"
                     /locus_tag="SI621_003899"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HEI4066557.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4458..6101
                     /gene="ecpD"
                     /locus_tag="SI621_003900"
     CDS             4458..6101
                     /gene="ecpD"
                     /locus_tag="SI621_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HEI4066558.1"
                     /translation="MRVNLLIAMIIVALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRSGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6070..6780
                     /gene="ecpE"
                     /locus_tag="SI621_003901"
     CDS             6070..6780
                     /gene="ecpE"
                     /locus_tag="SI621_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HEI4066559.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGEVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7093..7422
                     /locus_tag="SI621_003902"
     CDS             7093..7422
                     /locus_tag="SI621_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEI4066560.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7671..8285)
                     /locus_tag="SI621_003903"
     CDS             complement(7671..8285)
                     /locus_tag="SI621_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HEI4066561.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8691..9380
                     /gene="paoA"
                     /locus_tag="SI621_003904"
     CDS             8691..9380
                     /gene="paoA"
                     /locus_tag="SI621_003904"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HEI4066562.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLDVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPENLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9377..>10720
                     /locus_tag="SI621_003905"
     CDS             9377..>10720
                     /locus_tag="SI621_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEI4066563.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGVLAVITASNAGALGKGD
                     KNTARLLGGPTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVS
                     QPPEDTPDKNVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTS
                     NQMIDWCRTDLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRP"
BASE COUNT         2732 a         2826 c         2780 g         2382 t
ORIGIN      
        1 accgttctga taaatacatc tggtctcccc atgacgccta cttctataaa ggactatctg
       61 aactgattgt ggatatcgac agattaattt atctatcgct ggagaaaatc agaaaagatt
      121 tcgtgtttat caatctcaat acggattctt taactgagtt tataaaccgt gataatgagt
      181 ggttatccgc ggtaaagggg aaacaggtcg tattgattgc ggccagaaag tcagaagcct
      241 tagcaaatta ttggtattac aacagcaata ttaggggcgt ggtatacgct ggactgagtc
      301 gtgatattag aaaagaactg gcctatgtga ttaatggcag gttcctgaga aaagatatta
      361 agaaagataa aatcactgac cgggaaatgg aaattatccg catgacggct cagggaatgc
      421 tgcctaaatc gattgccaga attgaaaatt gtagtgtgaa gacagtgtat acccatcggc
      481 gtaatgctga ggccaagctg tactcaaaaa tatataagtt ggttccgtaa actccaggca
      541 agttagtttt aaaaaatgac tcacagggac atcacgtcct caattcaact cgggaagaaa
      601 agcaatgaaa aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gcatgggtgt
      661 ggcgcaggct gctgacgtaa cagctcaggc tgtagcgacc tggtcagcaa cagccaaaaa
      721 agacaccacc agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc agtatgccga
      781 aggcattaaa ggttttaact cacagaaagg tctatttgac gtagctatcg agggtgactc
      841 aacggctacc gcctttaaac tgacctcacg tcttatcacc aacaccttaa cccagttgga
      901 tacctcaggt tccacactga atgtgggcgt tgattataac ggcgcggcag tcgaaaaaac
      961 tggcgatacc gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccact
     1021 ggctaacggt tacaatgcca gcaatcgtac caccgcacag gatggtttca ctttctccat
     1081 catcagcggc accaccaatg gtaccaccgc agtaaccgat tacagcactc taccggaagg
     1141 catctggagc ggcgacgtta gcgtacagtt cgacgctacc tggaccagtt aatctctctg
     1201 atgtaccagc aggggtaccc ccctgcttta ttcctggacg gaattgttat gaaaaagcac
     1261 cttctgcctc tcgctctgct gttttccgga atatctccgg cccaggcgct ggatgtcggc
     1321 gatatatcat cgtttatgaa cagtgacagc agcacgctga gcaaaacgat ccaaaacagt
     1381 accgacagtg gccgcctcat caatatccgt ctcgaacggc tctcttcacc gcttgacgac
     1441 gggcaggtta tcgcaatgga caagccggat gagttgctac tcactcccgc tagcttgctg
     1501 ctacccgccc aagccagcga agtgatccgc ttcttctata agggaccagc agatgaaaaa
     1561 gagcgctact accgcattgt ctggttcgat caggccctca gtgatgcgca gcgcgataat
     1621 gccaaccgca gcgctgtagc cactgcttcc gcccgcatcg gcaccattct ggtcgtcgcc
     1681 ccccgccagg cgaactaccg ctttcagtac gccaacggct ccctgacgaa tacaggaaat
     1741 gcgacgctgc ggatcctcgc ctacggcccc tgcctgaaag ccgccaatgg taaggagtgt
     1801 aaagagaatt actacctgat gccgggtaag tcgcgtcgtt ttacccgcgt ggatacggcg
     1861 gataacaaag gacgggttgc actttggcag ggtgataagt tcattcccgt gaaatagata
     1921 gctgtgcaga tggataacga caatgccttt acgacggttc tccccaggac tgaaagccca
     1981 gtttgccttc ggcatggtct ttttgttcgt tcagcccgat gccagcgctg ctgacataag
     2041 tgcgcagcaa atcggtgggg tgattattcc gcaggccttc agtcaggcgc ttcaggacgg
     2101 catgagcgtc ccgctctata ttcatcttgc cggtagccag ggtcgccagg acgatcagcg
     2161 aatcggcagc gcttttatct ggttggatga tggacagcta cgcatccgga aaatacagct
     2221 ggaagagagt gaagataacg ccagtgtcag cgaacaaact cgacagcagc tgatggctct
     2281 ggccaacgcc ccgttcaatg aggcccttac catccccctg actgacaacg cgcagctgga
     2341 tctcagcttg cgccaactgc tgctgcagct ggtggtcaag cgcgaagcgc tgggcaccgt
     2401 actacgctca cgtagcgaag acatcgggca gtccagtgtt aacaccctca gcagtaatct
     2461 gagctataac ttcggcgtct ataacaacca gttgcgtaac ggcgggagca acacatccag
     2521 ctatctgtcg ctgaataacg ttactgcgct gcgcgaacat cacgtggtgc tcgacggctc
     2581 actgtacggg attggtagcg gtcaacagga cagtgaatta tataaagcga tgtatgaacg
     2641 cgattttgct ggtcaccgat ttgccggtgg aatgctcgac acctggaact tgcagtcctt
     2701 agggccgatg accgccattt cagcagggaa gatttacggc ctttcctggg gaaaccaggc
     2761 cagctccacc atcttcgaca gcagccagtc agccacgcca gtgatcgcct ttttaccggc
     2821 ggcgggcgaa gtacatctca cccgtgatgg gcgactacta agcgttcaga acttcaccat
     2881 gggcaatcat gaagtggata cccggggtct accgtacggt atttacgatg tggaagttga
     2941 ggtgatcgtt aacggtcgcg tgatcagcaa acgcacccag cgggtcaata agctgtttag
     3001 ccgggggcgc ggcgtcggtg caccactggc gtggcagata tggggcggta gctttcatat
     3061 ggatcgctgg tcggaaaacg ggaaaaagac gcgaccagct aaagagagtt ggctggcagg
     3121 tgcctcaacc tccggctcac tgagtacgct tagctgggcg gcaacgggat atggatacga
     3181 taatcaggcg gtgggtgaaa cccgtctgac gctgccgctt gggggggcga tcaacgttaa
     3241 cctgcaaaac atgctggcca gtgacagctc atggagcaac atcgccagca tcagcgccac
     3301 tctacctgga ggcttcagtt ctctgtgggt taaccaggaa aaaacccgca ttggcaatca
     3361 attgcgacgt agcgatgccg acaaccgtgc aatcggcggc acactcaacc tgaactcact
     3421 gtggtcgaag ctgggtacgt tcagcatcag ctacaatgat gaccgccgct acaacagcca
     3481 ttattacacg gcagattact atcaaaatgt ctacagcggt ccctttggtt cgcttggcct
     3541 gcgggccggt attcagcgct ataacaacgg cgacagcagc gccaatacag ggaaatatat
     3601 cgctctcgat ctctcgctac cactgggcaa ctggtttagc gcagggatga cccatcaaaa
     3661 cggctacacc atggcaaacc tgtcagcacg caaacagttt gatgaaggaa ccattcgcac
     3721 tgttggtgcc aatctgtcac gagccatctc cggcgatacc ggtgatgaca aaaccctcag
     3781 cggtggggcg tatgcacagt tcgacgctcg ttacgccagc ggaacgctga acgtcaatag
     3841 cgcggcggac ggctacatca ataccaattt gaccgccaac ggcagcgtcg gctggcaagg
     3901 taaaaacatc gctgccagcg ggcggaccga tggcaatgct ggggtgatat tcgacactgg
     3961 cctggagaac gatggtcaga tcagcgccaa aatcaacggg cggattttcc cgcttaacgg
     4021 caagcgtaac tatctcccgc tctctcccta tggaagatat gaggtggagt tacagaacag
     4081 caaaaactca ctcgacagtt acgatatcgt cagcggtcgc aaaagccatc tgactctcta
     4141 tccaggcaat gtcgctgtca ttgagccaga agtgaagcag atggttaccg tctccggtcg
     4201 tatccgtgcg gaagacggca cactgctggc taacgcacgg attaacaacc atatcggccg
     4261 aacccgaacc gatgaaaacg gcgagtttgt catggacgtg gataagaaat accccactat
     4321 cgattttcgc tacagtggca ataaaacctg cgaagtggca ctggaactca accaggcgcg
     4381 cggtgccgtc tgggtcggtg atgtggtctg cagcggcctc tcatcgtggg cggcggtgac
     4441 gcagacagga gaagagaatg agagttaacc tactgatagc gatgataatc gttgcgctaa
     4501 tctggcctgc aaccgcgctc agagcggcag tgagcaaaac cacctgggcg gatgctccgg
     4561 cacgcgagtt tgtgtttgtc gaaaacaact cagacgacaa ctttttcgtc actcctggcg
     4621 gggcgctgga tccgcgcctg accggtgcca accgctggac cggtttaaaa tacaatggtt
     4681 caggaaccat ctatcagcaa agcctcggct acattgataa cggttacaac accggccttt
     4741 ataccaactg gaagtttgat atgtggctgg aaaattcacc agtttcatct cctttaactg
     4801 gcttgcgctg catcaactgg tacgctgggt gtaatatgac caccagtctt atcctgccgc
     4861 aaaccaccga cgccagtgga ttttatggcg cgacggtgac cagcggcggc gcgaagtgga
     4921 tgcacggcat gttgtcagac gcgttttacc agtatctgca acaaatgccc gtcggcagca
     4981 gctttacaat gaccatcaat gcctgccaga cctctgtgaa ctatgacgcc agcagcggcg
     5041 cacgctgtaa ggatcaggcc tccggcaact ggtatgttcg caacgtcacc catacgaaag
     5101 cagcaaatct acggttgata aatacccact cgctggcgga ggtgtttatc aacagcgacg
     5161 gagtaccgac tctgggcgaa gggaacgccg actgccggac gcaaaccatc ggcagccgtt
     5221 caggattaag ttgtaagatg gttaactata ccctgcaaac aaacggactc agcaacacct
     5281 caatccatat attcccggcg atcgccaact cgtcgttagc ctcggccgtc ggggcgtacg
     5341 atatgcagtt cagtctgaat ggcagttcat ggaaaccggt gagcaatact gcctattact
     5401 acaccttcaa cgagatgaag agcgcagact cgatctatgt tttcttctcg agcaacttct
     5461 ttaagcagat ggtgaacctc gggatcagcg atatcaacac caaagatcta ttcaactttc
     5521 gctttcagaa caccacatca ccggagtctg gctggtatga attttctacc tccaacacgc
     5581 tgattatcaa accccgtgat ttcagcatca gtattatctc cgatgaatat actcagacac
     5641 cgtcgcggga gggatatgtt ggcagcggcg agtcggcact cgatttcggc tatatcgtaa
     5701 ccaccagcgg taaaacagct gccgacgaag tgctgatcaa ggtgaccgga cccgcgcagg
     5761 tgattggcgg gcgctcctat tgtgtcttca gctccgatga tggtaaggcg aaagtaccgt
     5821 tcccggcgac gctttccttt attacccgca gcggagctac aaaaacctac gatgccgggt
     5881 gcgatgatag ctggcgggat atgaccgatg cgctgtggct gaccacaccg tggactgata
     5941 tctctggcga agtggggcag atggataaga ccacagtcaa attttcgatt ccaatggata
     6001 acgccatttc gctgcgtacg gtagatgata acggctggtt tggcgaagtc agcgcttcag
     6061 gagagattca tgttcaggcg acgtggcgta acattaacta aggccctgct gacagtggtc
     6121 tgtatgctgg cggcaccttt gacacaggcg atttcggtcg gcaatctgac tttttcactg
     6181 ccgtccgaga ctgactttgt cagcaaacgt gtagtgaata acaacaaaag cgcacggata
     6241 taccgtattg ccatcagtgc tattgatagc ccgggcagca gtgaattgcg cacccgaccg
     6301 gtggatggtg aactgctttt cgccccccgc cagctggcgt tgcaggctgg tgagagcgag
     6361 tattttaaat tttactatca tggtccacgg gataaccgcg agcgctacta ccgggtctca
     6421 tttcgcgagg tccccactcg taacctgaca aaacgcagcc ctaccggcgg tgaggtcagc
     6481 acggagccgg tggtggtgat ggataccatt ctggtagtac gaccacgtca ggttcagttt
     6541 aaatggtcct tcgaccaggt gacaggaacg gtaagtaaca ctggcaacac atggtttaag
     6601 ctactgatta agccaggatg tgattcgacc gaagaggaag gcgatgcctg gtatctacgt
     6661 ccgggagagg tggttcatca gcctgagtta cgtcagccgg ggaatcatta tctggtctat
     6721 aacgacaaat tcattaagat tagcgattcc tgtccggcta agcccccttc ggcggactaa
     6781 gacttatcca tcggtcaagg gaagaactcc acctcagaga ctcaaaaatc gtttaaataa
     6841 tgtggaagcg atcgtcaatg tcacctgtaa cgatgggctt tctcatatgt acaccatagt
     6901 gttcaggaga cgcggaaagg taccaggcag cctgctaacg agtggtttat tcacgaatta
     6961 gtacgtaaaa tcggtaacgg ctggaaatca ttcaatactc acactatcga aagttcccca
     7021 gccaaccgcg gtacgttctt acatacgatg taccgctgtt ctctttacga tttatagctg
     7081 tactggtgaa ttatgagcaa tctgaatcca tgcatgacgt gtggtgcctg ttgtgcattt
     7141 ttccgcgtct ctttttactg ggccgaagcc gacgatgctg gcggtaccat tcccgccagg
     7201 ctcactgaac aaatatcccc ttttcaccga tgcatgagcg gtaccaatca gaaaaacccc
     7261 cgatgtattg cccttgcagg aacaccgggc aaaaatgcct gctgcacgat atataaaaat
     7321 cgatcgtcca cgtgcagaga attcgccatg tctggtgaga acggagtcgt caatgaggct
     7381 tgcaatcgtg caagagctaa atacgggctg acaccgttat aaatatgcaa taattaattg
     7441 cactgccccg ccagctataa tcgcggggct tcgtttttca gggataacaa aacccgaaac
     7501 ttcttccatc tgccagcaac aatgcctctt cctctatagt gttctccgga tctacagcat
     7561 cctgaagtag cgcgatgtac tcacgggcta tctgcttcag aaagtaaaaa gcaatgtttt
     7621 taacctataa aaatggcgct gcatttgcgc catttttatt attcaatgca ttatctgttt
     7681 gagcctaaag ggatctcagg gtctggctca tgagtaattc tgtttcgtaa atctctgcga
     7741 ataatttcaa tggaccagaa ccagactaaa tgtccaaaaa tttcagaaac attctcatac
     7801 cacgggagat caaacagagg tggcgtcagt cccatgagag ggaatgaaat catatgaaca
     7861 aaaagttggg ctaaagcacc tgccagtaag ccctgccaga gtttaatttt tggaaatact
     7921 tcagcgacca cacaataacc gacagcaaac actatcgaaa agataatgtg cgtaacacca
     7981 acccagttaa agacatgccc ggcaaaggta taaacagccg cattgggatc tgtcagcccc
     8041 aaccagtctc gaagaaaaat atacggagga ttgagaaaat tacgggagca atcaatttgc
     8101 ccagcagccc tgattaatga ttccgggcca cacgctgcat taaacatatc caccgggcta
     8161 cgtggcggca atggaacttc agccccccac ttcacaaatg cggaaacaac gccagcaatc
     8221 agcccaatga atgcagcaag accataacgt ctgcggttcg gtggagtttg ttcaaatata
     8281 ttcatatcta ccctgcttgt accattatgt tatacacctc ttcaggagta ttcataaaat
     8341 aagacaaatg taaagaactg tattgttttg tataatgcaa tggtgactaa tcgccaatga
     8401 atataagcac catcatttct ccctattttt atattaaaag tgacaacgat ttacagggtg
     8461 atgctgagct gacaatcaca ggtttcctta tcggtttttg catcgcacaa ctaaagcaat
     8521 aaactagctc ccgtcatcgg cgaatatgaa ttaattagcc gggctcatag ctgcattcgc
     8581 gttttaagaa taatcctcct gctctcaccg actatgctta acgtttaaaa aagcatcagc
     8641 actctcgcaa cgcactctta ttttcccctt tagaataccg gaggcctggt atgagcaacc
     8701 aaggcgaata ccccgaagat aatcgggttg ggaagcacga gccgcacgat tttagtttga
     8761 cccgtcgcga tctgattaaa gtcagcgccg caacggcggc gaccgccgtg gtttatcctc
     8821 attctacgct tgcggcaagc gttccggcag ctacacccgc gccagagata atgcccctga
     8881 cacttaaggt gaacggcaaa accgagcagt tggacgttga tacccgaacc acgctactgg
     8941 acgctttgcg tgaaaatctg catctgatcg gtaccaagaa aggttgcgat cacggacagt
     9001 gcggagcctg taccgtgctg gtcaatggtc gaaggcttaa tgcctgcctg acgcttgcag
     9061 tcatgcatca gggggccgag atcaccacca ttgaaggcct gggttcacca gagaatcttc
     9121 accccatgca ggcggccttt atcaagcatg atggcttcca gtgcggctac tgcacctccg
     9181 ggcaaatttg ctcatcagta gcggtgctaa aagagattca ggacggcatt cccagtcacg
     9241 tcacggtcga tttggtttcc cctccagaaa gaacggccga tgagatccgt gaacgtatga
     9301 gcggcaacat ctgtcgctgt ggtgcatacg ctaacatcct tgccgccatt gaagacgctg
     9361 cgggggagat aaaatcatga aggcgtttac ctatgaacgt gtgaacaccc cggccgaggc
     9421 ggcacttagc gctcagcgcg tacctggcgc aaaatttatc gctggcggga ccaatctgct
     9481 ggacctgatg aagctggaaa ttgaaacgcc aacccacctt atcgatgtga acgggctcgg
     9541 gctcgataag attgaagtga ccgacgcggg tgggctgcgc atcggcgcac tggtacggaa
     9601 caccgacctg gcggctcacg agcgcgtgcg tcgtgattac gcggtactct cccgcgccct
     9661 gctcgctggc gcgtctggtc agttacgcaa tcaggcaacc accgcaggta atctgctcca
     9721 gcgcacgcgc tgcccctatt tttacgacac caatcagccc tgcaataagc gcctgcccgg
     9781 gagcggctgc gcggcacttg aaggctttag ccgtcagcac gcggtggtag gcgtaagcga
     9841 agcctgcatt gccacccatc cgagcgatat ggcggtcgca atgcggttgc tggatgcggt
     9901 ggtggaaacc atcacgccgg agggaaagac tcgcagtatc acactggctg atttttatca
     9961 ccctccggga aaaacgccgc acattgaaac cgccctgctt cccggtgagc ttatcgttgc
    10021 ggtgacgtta cctccgccgc tcggcggaaa acatatctac cgcaaggtgc gcgatcgcgc
    10081 ctcctacgcc tttgccctgg tatcggtcgc ggcgattatt cagcctgacg gcagcgggcg
    10141 cgtcgcgctg ggcgtactgg ctgtcattac cgccagtaac gccggggcac tcggcaaagg
    10201 cgacaaaaac accgccaggc tgttaggcgg ccccactatt gaacactatc atcaggccat
    10261 tgcgctggta gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc
    10321 gcactatcgc cgtaataaag gagcttactc cctggcggac gagaaacagg ccgtcagtca
    10381 gccgccggaa gacacgcccg ataaaaatgt tggtgacttt gacggggctt tcacctccgc
    10441 tgcggtgaag attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc
    10501 gcatgcctcg atggccgtct gggatggaaa taagcttacc ctctggacct caaatcagat
    10561 gattgactgg tgccgcaccg atctggcaaa aacgctgaaa gtgcccgtgg agaatgtgcg
    10621 tattatctcc ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc
    10681 gctgctggcg gccctcgccg cccgtgcggt gaaacgtccg
//
LOCUS       DARNVM010000165        10709 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.165, whole genome shotgun sequence.
ACCESSION   DARNVM010000165 DARNVM010000000
VERSION     DARNVM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.165"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1221
                     /gene="garD"
                     /locus_tag="SI621_003906"
     CDS             <1..1221
                     /gene="garD"
                     /locus_tag="SI621_003906"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEI4066564.1"
                     /translation="YTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLL
                     PKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQ
                     PERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRETC
                     PASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPR
                     AVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKS
                     AIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPV
                     IKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQWG
                     LHNQLAVFNPAPVT"
     gene            1370..1705
                     /gene="prlF"
                     /locus_tag="SI621_003907"
     CDS             1370..1705
                     /gene="prlF"
                     /locus_tag="SI621_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HEI4066565.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDTQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            1705..2169
                     /gene="yhaV"
                     /locus_tag="SI621_003908"
     CDS             1705..2169
                     /gene="yhaV"
                     /locus_tag="SI621_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HEI4066566.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH"
     gene            complement(2224..3033)
                     /gene="agaR"
                     /locus_tag="SI621_003909"
     CDS             complement(2224..3033)
                     /gene="agaR"
                     /locus_tag="SI621_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HEI4066567.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3282..4562
                     /gene="kbaZ"
                     /locus_tag="SI621_003910"
     CDS             3282..4562
                     /gene="kbaZ"
                     /locus_tag="SI621_003910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HEI4066568.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4585..5058
                     /gene="agaV"
                     /locus_tag="SI621_003911"
     CDS             4585..5058
                     /gene="agaV"
                     /locus_tag="SI621_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HEI4066569.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5069..5848
                     /locus_tag="SI621_003912"
     CDS             5069..5848
                     /locus_tag="SI621_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEI4066570.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5838..6716
                     /locus_tag="SI621_003913"
     CDS             5838..6716
                     /locus_tag="SI621_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEI4066571.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            6734..7168
                     /locus_tag="SI621_003914"
     CDS             6734..7168
                     /locus_tag="SI621_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEI4066572.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7165..8298
                     /gene="nagA"
                     /locus_tag="SI621_003915"
     CDS             7165..8298
                     /gene="nagA"
                     /locus_tag="SI621_003915"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEI4066573.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRDGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTDVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            8649..9803
                     /locus_tag="SI621_003916"
     CDS             8649..9803
                     /locus_tag="SI621_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HEI4066574.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMVSCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            9816..10676
                     /gene="kbaY"
                     /locus_tag="SI621_003917"
     CDS             9816..10676
                     /gene="kbaY"
                     /locus_tag="SI621_003917"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HEI4066575.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2524 a         2797 c         2863 g         2525 t
ORIGIN      
        1 tacacctttg agggctatcg caatgccgat ggcagcgtgg gcactaaaaa cctgctcggc
       61 atcaccacca gcgtccactg tgtggcaggc gtggtggact atgtagtaaa aatcattgaa
      121 cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac
      181 ggttgtggcg tggcgattaa cgcaccggcg gcagttgtgc ctattcgtac cattcacaat
      241 atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag
      301 ttgcagcctg aacgcctgct gactggaacg gatgatgtgc aagctattcc agtagaaagc
      361 gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat
      421 attttgcagg tcgccgaacg ccatttgcac aaactgaatc aacggcagcg agaaacctgt
      481 ccggcttcag aactggttgt cggcatgcag tgcggcggta gcgatgcgtt ttctggcgta
      541 acggcaaacc cggcggttgg gtatgcgtct gatctactgg tgcgctgcgg cgcaacggtg
      601 atgttttcag aagtcacgga agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg
      661 aacgaagagg tcggtaaacg gctgctggag gagatggagt ggtacgataa ctatctcaat
      721 atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca acaagaaagg cggtctggca
      781 aacgtggtgg agaaagcgct cggctccatt gctaaatcgg gtaaaagtgc aattgttgaa
      841 gtgctgtcgc ctggtcaacg cccgactaaa cgcggattaa tttacgccgc gacgccagcc
      901 agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg
      961 accggtcgcg gtacgccgta cggcctgatg gcggtacccg tcattaaaat ggcgacccgc
     1021 accgagctgg cgaaccgctg gtttgattta atggatatta atgcgggcac catcgctacc
     1081 ggcgaagaaa ctattgaaga ggtgggctgg aagttgttcc actttattct cgacgtcgcc
     1141 agcgggaaga agaaaacctt ctcagatcaa tggggactgc ataaccagct ggcggtgttt
     1201 aacccggcac cggtgacctg atttcttttg gaacgtccct cgcaaaacat ggccttagtg
     1261 ccatgttttt attgtttaaa gcccccacgt acattaataa tgcatttgca ttaccttagt
     1321 tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtgaatca tgcccgctaa
     1381 tgctcgctct cacgctgtac tgaccactga atcaaaggtc acgatacgcg gacaaacaac
     1441 tatccccgcg ccagtgcgtg aggccttaaa actgaagcca ggccaggaca gcattcatta
     1501 cgaaattctg cctggtgggc aagtatttat gtgccgactg ggagatgaac aggaggatca
     1561 tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc cgcaaaaaac
     1621 tcgtccattc gacactcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga
     1681 tgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta
     1741 tatgctcatc cctgttttca ggaaacctac gacgctttag ttgccgaagt cgagacatta
     1801 aagggaaaag atcctgaaaa ttatcagaga aaagccgcca caaagttatt ggcggtagtc
     1861 cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc
     1921 aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg tgctggtcgt
     1981 tatcgtctct tctttcgtta cagcgaaaaa gagaaagtca tcattctggg atggatgaac
     2041 gatgaaaaca cgctgcgcac ctacggtaaa aaaacagatg cctataccgt attcagcaaa
     2101 atgttaaaaa gaggacatcc tcctgccgac tgggaaaccc tcacccgaga gacagaagaa
     2161 gcccattgat ggtgttcaca atgcccgccg aacgcattcg tgttgaccag cgggcaccct
     2221 ttctcactcc ccgaccagaa tcacttcaac cccagccttt cgcaggcctt ccagactatc
     2281 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     2341 taaactggaa cggttgaact tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     2401 gcacatccga cggtttaaac gggcttcatc ttcattatgc gtgctgacgc cgcgctccag
     2461 atcgatcgca tctacgccaa gaaacagcat atcgaagtgg taattttgca gcgattgttc
     2521 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
     2581 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     2641 tacgtcagtg tgcttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag
     2701 gatcacccga tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     2761 tgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
     2821 gcagatcaac gcgccgccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
     2881 atcgttgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     2941 acttccttgc tggcgcaggc gttgaatgat ctgctctcgt cgctcactgg tgcctgtcac
     3001 tcgcttctcg cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
     3061 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttacccat
     3121 tggcgcaagc tactctcgtt tcagtggctt tcattatgtt tcttttgtga atcagatcag
     3181 aaaaccatta tctttcgttt tatttttatc tcaccatggc gcagtatcaa ctgaaacaaa
     3241 acgaaagatt aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
     3301 tggtgagaca gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt tccgcacatc
     3361 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
     3421 aagcaacctc caatcaggta gaccagttcg gcggctatac cggaatgacg cccgccgatt
     3481 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgttgattc
     3541 tgggtggtga ccatctgggt ccaaaccgct ggcaaaacct gccagccgct caggcaatgg
     3601 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
     3661 attgcagcat gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac
     3721 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg
     3781 atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa
     3841 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
     3901 cctttgaaaa gcagggtctg agtgccatct ggccacgcat cattgctctg gtggttcaac
     3961 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgtcaaa gcgaccgcct
     4021 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
     4081 aaacgccaca atcgttgcgc cagctggtga ttgaccactt tgccatcctg aaagttggcc
     4141 cggcgctgac cttcgccctg cgtgaagctc tgttctctct ggcggcgatt gaagaagaac
     4201 tggtaccagc aaaagcctgt tctggtctgc gtcaggtgct ggaaaacgtg atgctcgacc
     4261 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcacgtcgc ctggcgcgtg
     4321 gttacagcta ctcagatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
     4381 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt
     4441 atttgccgct gcaatacgtg aaagttcgct ccggcgagct gcagccaaca ccacgggaac
     4501 tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
     4561 aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagccggat tgatgagcgt
     4621 ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg
     4681 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
     4741 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa catccaccgc
     4801 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ctgccgattt cctgacgctg
     4861 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
     4921 aagcaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
     4981 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
     5041 ctctttaaat tactttgagg gattcatcat ggaaatcagt ctgttgcagg cattcgcgtt
     5101 gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcttaaccc atatgcaccg
     5161 cccggtggtc ctcggcccgt tagtcgggct ggtacttggc gatctgcata ccggtatttt
     5221 aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctggcag gcgcacaacc
     5281 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
     5341 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
     5401 cttcctgttc tcggtgatgt ctggcgtaat gtcccgctgc gaccggatgg cggagaacgc
     5461 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
     5521 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
     5581 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
     5641 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct acttcatcct
     5701 gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg ccgccgcgct
     5761 ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
     5821 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agtgaaaacg
     5881 aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcagagc attggcgcag
     5941 agctgacaaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
     6001 ttaactacga acgtatgcag gcttccggct ggttgtacgg tctgctgcct gctctgaaaa
     6061 agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
     6121 atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta
     6181 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg ctcggcggga
     6241 ttggcgatgc aatgttctgg ctgacgctac tgccgatttg tggcgggatt ggtgcaagcc
     6301 tcgcgctaca aggctctatt cttggcgctg tcgtctttat tgtgctgttc aacgtggtac
     6361 acctggggct gcgttttggt ctggcgcatt atgcttatcg catgggcgtg gcggcgattc
     6421 cactcattaa agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt gggatgacgg
     6481 taatcggcgc gctggtagca acctatgttc gtttaagcac cacgctggaa atcaccgctg
     6541 gcgatgcagt ggttaagtta caggctgatg ttatcgacaa actgatgcct gccttcttac
     6601 cgctggtcta caccctgacc atgttctggc tggtacgccg cggctggagt ccgctgcgcc
     6661 tgattgcagt taccgtggtt ctcggtatcg tcggtaaatt ctgtcatttc ctttaaatac
     6721 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcggatttg ccagcggcat
     6781 ggaaaaagca atgaagcaaa tcctcggcga gcaatcgcag tttattgcca tcgattttcc
     6841 cgaaacctca agcaccgcgc agcttacatc gcagcttgaa gaggccatcg cccaactcga
     6901 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
     6961 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc accggcacca atttgcaact
     7021 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagagttcc gtgtgcaggc
     7081 gctggagtgt ggacaccgtg ggctgaccag tttggtggac gagttaggcc gctgccatga
     7141 agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
     7201 gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtaat cgcagcaatc
     7261 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc
     7321 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtgctc
     7381 gacaagctgg caatgcacaa agcacgcgaa ggtgtcggca gttggttacc gaccaccgta
     7441 accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
     7501 gacggacctg gtgcgcaagt gctggggagt tatctcgaag gaccatactt cacgccgcag
     7561 aataaaggcg cgcatccgcc ggagctgttt cgcgagctgg aaattgccga gctggatcaa
     7621 ttgattgccg tttctcagca caccttgcgc gtggtagcgc tggcaccgga aaaagaaggg
     7681 gcattgcagg ccatccgcca tcttaaacag caaaacgtac gggtgatgct ggggcatagc
     7741 gcggcgacct ggcaacaaac ccgtgccgcg tttgacgctg gtgccgacgg cctggtgcat
     7801 tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg cgcgggatta
     7861 acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca tccggcggcg
     7921 atgtcgctgt gttgttgctg tgcgaaagag agaatcgtgc tcatcaccga cgcgatgcag
     7981 gcagccggga tgccggatgg tcgctatacg ttatgtggcg aagaagtgca gatgcacggt
     8041 ggcgttgtcc gtaccgcgtc cggtggactg gcgggcagta cgctgtctgt tgatgcggca
     8101 gtgcgcaata tggtcgagtt gacggacgta acgcctgcgg aagccatcca tatggcgtcg
     8161 ctgcatccgg cgcgtatgct gggtgttgat gatgttctgg gatcgcttaa accgggcaaa
     8221 cgcgccagcg tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg gattcagggt
     8281 caattagctt cgttttgatg gtttgctcct ttattgggcc ttcacttccc ccgtaaggcc
     8341 tttctttttc tttctttttg atctgtacag cggtgtcgga tgcgacgctg acgcgtctta
     8401 tgcgacctac ggtacgccgc atccggcaat cggtgcacat agcaacgtca tcatgcaatt
     8461 ctttaatctt cccttgtctt ttctttcaac gatcacaaat ttcgttttat ttcttttttc
     8521 tccattgaac tttcagtttc ttttctatag attttaatca acgaaagaca tcaccaagtg
     8581 aaatgaaacg aaaggtaagc gaaagcgaca acgcccgacg tcaagttcat cagactaagg
     8641 attgagttat gccagaaaat tacacccctg ctgctgccgc aaccggtaca tggactgaag
     8701 aagagatccg ccatcagcct cgcgcatgga tccgttcact caccaacatc gacgcgctac
     8761 gttccgcgct caataacttc cttgaaccgt tactgcgcaa agagaatctg cgggtaatcc
     8821 tgaccggagc cggaacctcg gcatttatcg gtgacatcat cgcgccgtgg ctcgccagcc
     8881 atacaggtaa aaacttcagc gccgtaccga ccaccgatct ggtcaccaat ccgatggact
     8941 acctgaatcc agctcatcca ctgctgttga tctccttcgg tcgatccggc aacagcccgg
     9001 aaagcgtcgc cgccgtggaa ctggcaaatc aatttgtacc cgagtgctat cacctgccga
     9061 tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat caacagcgac aacgcgtttg
     9121 ccctgctgat gcccgcagaa acgcacgacc gcggctttgc gatgaccagc agcattacca
     9181 ccatgatggt cagctgcctc gcggttttcg cacctgagac gatcaacagc cagagcttcc
     9241 gcgacgtggc ggatcgttgc caggcgatcc tgacctcact gggcgatttc agcgaaggtg
     9301 tgtttggtta cgcaccgtgg aaacggatcg tttatctcgg cagcggtggc ttacagggcg
     9361 cagcacgcga gtcggcgctg aaagtactgg aactgacggc gggtaaactg gcggcctttt
     9421 atgattcccc gaccggattc cgtcatggcc cgaaatcgct ggtcgataac gaaacgctgg
     9481 tggtggtatt tgtctccagc cacccttaca cccgtcagta tgatcttgat ctgctggctg
     9541 aacttcgccg cgataaccag gcgatgcgcg taatcgctat cgccgcggaa agcaacgacg
     9601 ttattaccgc cggtccacat atcatcctgc cgccgtcccg tcactttatc gacgttgagc
     9661 aggcattttg cttcctgatg tacgcccaga cgtttgcact gatgcagtcg ctgcacatgg
     9721 gcaatacgcc ggatacccca tcagccagcg gcaccgttaa ccgcgtggtg caaggcgtaa
     9781 tcattcatcc gtggcaggca taagaggatc gcattatgag cattatctcc accaaatatc
     9841 tgttacagga cgcccaggcc aatggctacg cggtgcctgc ttttaacatt cataacgccg
     9901 agacgatcca ggcgatcctc gaagtgtgca gtgaaatgcg atcgccggtg atcctcgccg
     9961 ggacgccggg gacctttaaa catatcgcgc tggaagagat ctacgccctg tgtagtgcct
    10021 attccacaac ctacaacatg ccactggcgc tgcatctcga ccaccacgaa tcgctggatg
    10081 atattcgccg taaagtccac gcaggcgtgc gcagtgcgat gatcgacggc agccacttcc
    10141 cgtttgccga gaacgtaaag ctggtgaaat cggttgtcga cttctgccac tcacaggatt
    10201 gcagcgtgga agcagaactg ggccgcctgg gcggtgttga agatgacatg agcgttgacg
    10261 ccgaaagtgc attcctgacc gatccacaag aagctaaacg ctttgtcgaa ctgactggcg
    10321 tcgacagcct ggcggtagcg attggtacgg cgcacggctt atacagcaaa acgccgaaga
    10381 ttgatttcca gcggctggcg gaaattcgtg aagtggtgga tgttcctctg gtgctgcatg
    10441 gtgccagcga tgtcccggat gaatttgtcc gtcgcactat tgaacttggc gtcacaaaag
    10501 tgaacgttgc cacagaatta aaaatagcct tcgctggcgc ggttaaagcc tggtttgcgg
    10561 aaaatccgca gggtaatgat cctcgttatt atatgcgcgt cggtatggat gcgatgaaag
    10621 aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa tcgaatttca gcataatcat
    10681 tattttttta tgatttatcc caatgtact
//
LOCUS       DARNVM010000166        10639 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.166, whole genome shotgun sequence.
ACCESSION   DARNVM010000166 DARNVM010000000
VERSION     DARNVM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.166"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(14..586)
                     /locus_tag="SI621_003918"
     CDS             complement(14..586)
                     /locus_tag="SI621_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEI4066576.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(648..1172)
                     /locus_tag="SI621_003919"
     CDS             complement(648..1172)
                     /locus_tag="SI621_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066577.1"
                     /translation="MKLTYKIVIAAFFFSAFGALVWSANHYHSKYQAEKLRADKAEDE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            complement(1156..1632)
                     /locus_tag="SI621_003920"
     CDS             complement(1156..1632)
                     /locus_tag="SI621_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEI4066578.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            complement(1636..1977)
                     /locus_tag="SI621_003921"
     CDS             complement(1636..1977)
                     /locus_tag="SI621_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HEI4066579.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            complement(2381..2815)
                     /locus_tag="SI621_003922"
     CDS             complement(2381..2815)
                     /locus_tag="SI621_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HEI4066580.1"
                     /translation="MRNIQQVLERWGGWAADSNTTVSWAPIAAGFKGLVVSSSPGRLS
                     CCDDDGLIIDACVCRLQQVRKPEELDVIMLYYVYGLSKREIGRRRRCSEGFIRQQLQV
                     AEGFIEGCLCMLGVSLQMDPEVEIQRDEKSISALRKNCATLV"
     gene            complement(3102..5288)
                     /locus_tag="SI621_003923"
     CDS             complement(3102..5288)
                     /locus_tag="SI621_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEI4066581.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTAHLLPVVCNPSATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFAHWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGESPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSIEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRSDFVDIDIRFDQFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAITWLNGLEWDGVPRIECFYHTHFGTAE
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKKCKVWEPLEPRGTT"
     gene            complement(5292..5492)
                     /locus_tag="SI621_003924"
     CDS             complement(5292..5492)
                     /locus_tag="SI621_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEI4066582.1"
                     /translation="MSGLTPQEMVKSLIDSGYTQNQIAEIAGVKQSSISRLLTGVHSD
                     PRFSTVRAIEKLFQEVTARQKV"
     gene            5634..6308
                     /locus_tag="SI621_003925"
     CDS             5634..6308
                     /locus_tag="SI621_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4066583.1"
                     /translation="MIDTTDILSQNIKYLMDKAKISSITELARRLQLNQPTLHRLVSG
                     EVKDPKYATLKQIADYFHVSPIDLAERSLQEMEKEDATGIKTYISLRFNKVPVLGNTQ
                     LGVGGLWSDTQYSVGSSDGFIYWPTKDEDAYALKCVGDSMMPRIKEGEFVIVEPNHDY
                     TPGDEVLVVTREGEVMVKTFLFERDGLFHLMSVNEDHPPVRVPRENIEKIHYVAGIAK
                     SALRMY"
     gene            complement(6684..7253)
                     /locus_tag="SI621_003926"
     CDS             complement(6684..7253)
                     /locus_tag="SI621_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066584.1"
                     /translation="MSEEKGILSRITESLSGVGGAIKSAVGAAREIQNLTVDYAVKEK
                     THILLDKLMDVQMQQMSLQELLIAAKEKIVELENEKVKQENWAAEAASYELYQPMRGT
                     LVYRSKLSADADQSPVYICPNCYEQKRKSILQAEGLVTKPGLGRAVNMVCSYCRASYL
                     FNRNALEARLPSEDENPGKAITDYDPYNQ"
     gene            7478..7627
                     /locus_tag="SI621_003927"
     CDS             7478..7627
                     /locus_tag="SI621_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066585.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            7624..8526
                     /locus_tag="SI621_003928"
     CDS             7624..8526
                     /locus_tag="SI621_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066586.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAVFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            8529..9830
                     /locus_tag="SI621_003929"
     CDS             8529..9830
                     /locus_tag="SI621_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEI4066587.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDNTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            9846..10394
                     /locus_tag="SI621_003930"
     CDS             9846..10394
                     /locus_tag="SI621_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEI4066588.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWEPIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
BASE COUNT         2856 a         2772 c         2407 g         2604 t
ORIGIN      
        1 ggtaacggca tacttagccc atcatttttc tatacgcctc tgcagcctgc tccggcgtta
       61 agttggtaat ttctgttctg acgggtcctc catcagcgcc agtcacttca tttttgacgt
      121 tgtctttaaa cgcctgaaca gaaacatgac gcccaagcaa ctcaaggttt ttaaccttat
      181 caggccactt aatcttttta aggatcccga ccatttctct gtcatctcct cgcccctcaa
      241 acatttcagc gaggttaaat ccactcaggt accgacgcca cgattccggc cacgcagaca
      301 gaggcttaat acttaaatcg tcctccagga tgtcagccac atcgagcctg tcgatctcaa
      361 ccagtcgcat cagcacataa ttcgcatcaa tgcccagttg atcaatacgc tcctgcttta
      421 gctcgttaat acgggcgcgt atctcaggtt taccgtatag ttcagccccc gtaacatgtg
      481 ctcgcctgga gacgtagcct gcgcgaatag ctgcttgtgt agcattcaga tcgacaagaa
      541 actcgcgaca aaacacctcg tgttttgctt tcagcttctt agtcatttta ttttccagtt
      601 atcaggtcat tatcgaagcc cctcctggaa gagcttctgt aatgctatta ccgggactgt
      661 tctatttgtc ggacaccagc caactggtta ttcgccttct cgatggctgc caacagtggg
      721 ttaatccaca gaacagcctg gcaatatgtc aacgttctgg tggtagtggc acgatcaccg
      781 gctgagtcaa tgtccccgga atcggcgtgc aatgcgctgg cgcgtaaacg gttcgcgtag
      841 ttgagcaacc cgacagcaat atcaacagga acagggaaat cacagttctt ttcacggagc
      901 agaacctcac ggtatttgat gactgtcttc tcgtgaccga tgtcgaccag agaatttaat
      961 cggcttgcgt tttctgctat ctggttaaaa cgattgaagt ttaatgcctg attagctatc
     1021 actttccctt gatattcagc ttcatcttcc gctttatcag cccgcaactt ttctgcctga
     1081 tacttgctgt ggtaatggtt tgcagaccag acgagcgccc caaaggcaga gaagaaaaat
     1141 gccgcgatga caatcttata agtcagcttc atttaccacc ccaccagcat ctttaaacct
     1201 ggaaatcagg tcaccgattt tatgttcata ctgaccgtaa cctgcaccag gtaacgacgc
     1261 ccagatattg ctgcaacggt cgattgcctg acgaatatcg ccacggtcaa tcatcggtaa
     1321 agcgccacgc tctttaatct gctgcagcgc cacagcgtcc tgactttctg gcgaaaaatc
     1381 tttcaggcca agctgcttgc ggtaggcatc ccaccagcgt gaaagaagct gataccggcc
     1441 tgcggctgtt gatttaagct tcgggtttag cgtgacaagt ttgcgagggt gatcggagta
     1501 atcagtgaac aactcacctc cgacgataac gtcataaccg tgattacgtg tcggttgtcg
     1561 cccgttatcc gttccttctg accatgccac catatccagg aaagctttac gttgggaatt
     1621 tagtgtctgc atgaattact ccttagagcc accaaacttg ttaccgatta ctcgcattgc
     1681 agccccacga atagcatcga caccgatcag cccaacacca ccaccaatgg caacagaaag
     1741 agatttaggc catccaacat actcaagagc ggatgcaaag gtcagcgtca gagcaccaca
     1801 gagcaaaatc tcaagcgttt ttcgtttcca gccgccgcca ccgccaaaat aggcaatgcg
     1861 caagccagcc ataacaattg acataaccac tgcgcccaac ggcgtatctc cacgccacca
     1921 actttgtaag agttccagta agtcaggcca ggaatgagga gcattgtgca ttttcatact
     1981 tcccacctcc gccattacgg ggtgttgttg aaaggcgggc cctgcgtata cgcccgtagg
     2041 attgggttat gagccgtcct tcggtgggcc ctgaatacaa aaaaagttcg ccatagcgaa
     2101 ccttgttaaa tttgttaaaa aaggaagtta tttaacataa tgtacgttat aggaaccaca
     2161 cgatcaccgc tcgcaataga tttgcgatga aaggcctatt taatcaactt aagtggtcca
     2221 gaatgacgaa attcgagtga ataaaaggtg cataaaaaag ggcaaaaact gcatagcgtt
     2281 ttttcgcgac gaaaaccctg ttttattaac ttttactgag aatagagcat taaaaaagcc
     2341 ccaatgattt ggagctcttc gttgaggtca gaaattcaac tcataccagc gtagcgcagt
     2401 ttttgcgtag cgcactaata cttttttcat ccctttgaat ttcgacctca ggatccatct
     2461 gaaggctcac acccagcatg cagagacacc cctcaatgaa tccctctgct acctgcaact
     2521 gctggcggat aaatccttca gaacagcgac gccgccgccc tatctcacgt ttgcttaacc
     2581 cgtagacgta gtacagcata atgacgtcca gttcctcagg cttacggacc tgctgcagcc
     2641 gacagacgca agcatcgata atcaggccat cgtcgtcaca gcaactcagc ctgccaggcg
     2701 agctgctgac taccagccct ttaaatcccg ccgctattgg agcccagctc actgtggtgt
     2761 tgctatcggc agcccatcca ccccagcgct ctaaaacctg ttgaatatta cgcatagctc
     2821 tgacctctac gttttgctac gaaaaatttt tctgagaact acgtctgttt atccgtgggt
     2881 gtggataacg cagttccgat ccgcatattg catgtatcaa catgcaaaaa gttgctaatt
     2941 cggagtggaa ccacctgccc ccacctggaa ccaccttttc ctaacctttc cctcaatcga
     3001 cttatatata tatggggttt ctagtagaaa ggcggttcca gtggttccag tagttccgcc
     3061 ccgcttgcca caaggtctgc aaggtggaac caccttctct tctaggtggt tccgcgtggt
     3121 tccagcggtt cccaaacctt gcattttttc cccccaattc gcctttgggc acgcttatac
     3181 ccgcaatttt gcaaaacatt actaattcgc atttcttcgc gttttccgat gcggtcagga
     3241 tttagcccaa tcgcatcacg caaaacatca cttgcgcgta aaaattcgca atttcgcgga
     3301 agttcattag tcatcaggtc gggcgtgtcg agccatttct ctaccgtttc aagccaagca
     3361 tccttgatgg tgtactgttc atggacactc gctgccagtt gttcagcctc acggaactgg
     3421 atacccccca gacgctgaaa cacctcacga gcctcagccc aaagtaaaag aagatctctt
     3481 tttatcgctt tcacgtcgac tttcgacact tccacgggga gccagcgacg gttaccagtc
     3541 ttgtccgcaa ggaattcgtc ctcattagtg gtaccaacga acaccaggcg acgaggaaac
     3601 tgggtggcga actcccggta tttcgggatc cagttttcgt gagtacgcgt cacaaacgct
     3661 ttgattgact ccagctcttt ggtattaagt ccgcgcagtt caccaatctc tgccaccagc
     3721 cgaccacgca tttttcgagc gagatcatcg tctttttcgg caaaagagat ttcagtaaaa
     3781 aacgcaggat cagggctcag tgcctccact ccggaagact taccgcagcc ctgcggacca
     3841 acgaggatcg gcaccatatc cgctttgatg cctggctcca gtactcgccc cgccaacgcg
     3901 gtccacatgt acatagacac cgcacgggta taaggcgttt cggcggtacc gaagtgcgta
     3961 tggtagaaac attcgatgcg cggcacgcca tcccactcca gcccgttcag ccaggtgatc
     4021 gctgaatcga atggttgttc atcggctgca agtaacacca catcgcgaat aagttcacgg
     4081 ccaacaggtt taaatccccg cttttccatc gtgatgcgca ggcgcgcata atccgcatcg
     4141 gtgaatgccc gccattgtcc ggatcctgcc ggggcaaaca tgatttcgtc gcggaactgg
     4201 tcaaagcgaa tatcgatgtc cacaaaatca gagcgtacta ctgctttggc tgcattgctg
     4261 atggttgcct cgatacgccc ccatttatca cgctcgaaag caggcagcgg taaaggttcc
     4321 gccacttcga tgctggtcag atcttcgaaa tcgtcgttgc ggatcccgat ggcattcagg
     4381 aaatctccgt catcacgatg cgcgcaactg gcatgcaggc atttaaaatg cccctgctca
     4441 aagcctgcgg ttcccgcagg aaaataaact gtgcttgttg gatcgcctcc gctactgtgg
     4501 ccgtcttcaa acggacagcg gatatatcgt tcaccgtttg cgccatccag cagcgtccag
     4561 ccattggcat caagatattc agctgtatca tccgtcgcgc cgggcgtgaa tgctgaacgg
     4621 tcgcgcatct tcgtgttgcc cgcttcggtg gttaccgaca cagggagttg ttcagccagg
     4681 cgctgccaca gcgtttcaag ctgctcacct gtaatagccg gaggttcatc cggcaaacca
     4741 ccgtcccatt caatacgcgc gccgctgctg tgcgtaccac aggcaacgaa ctgctgcccg
     4801 ttcgccagca actcgataat gcccatatcc cccgctaggc gatggatacg cttacggaaa
     4861 tcaccatcaa cggccagcag atacagacac ttattactgt ttgctcgcca gcgtcgcggc
     4921 ggcgactcac ccagaagttg cacaagtgtt ttgcgaatat ctgcctgaat gtcttcatct
     4981 tcgctatcgc agtccagcgc cagccagcca tggcctgtac gcacgcagat gccataatcc
     5041 ggttcattcg accagtgggc aaaatcatgc tcagtaacaa catgcccggt ccattgagca
     5101 ataccggtga cctggcggtc ccggttatag cgactcggcg tcttacccaa cgctttcaat
     5161 ttactatcag gggatatggt cgcactgggg ttacatacaa ctgggagaag atgagcagtc
     5221 cgccccaata ccaggtcgaa atgaaaccac tcatcaggcg tagctcccca tggtttgcta
     5281 tcggacatgg gttacacctt ttgtctagct gtcacttcct gaaaaagttt ttctatcgca
     5341 cgtactgtag aaaagcgagg gtcagaatga accccggtaa gaagccgact aatagaagac
     5401 tgcttcacgc ctgcaatttc ggcaatttgg ttttgggtat accctgaatc aatcaagctt
     5461 ttgaccatct cttgtggtgt tagtccggac atatttaagt ctccactttc gtctatagcg
     5521 caaatttata cgaaaacgga ttactcagca atacagcaat tctatgtcat cctagattaa
     5581 aatatccgat aacgcataat caccgtccct gtatacaaca tggatgaatc caaatgattg
     5641 atactaccga tatcctttct caaaacatca agtacctgat ggacaaagcc aagataagct
     5701 cgataactga gctggcacgt cggttacagc tgaaccaacc aacgctacac aggctggtat
     5761 caggagaggt gaaagatccg aaatacgcaa cgttaaagca gatcgccgac tacttccacg
     5821 tgtcaccaat agacttggca gaaaggagcc ttcaagaaat ggagaaagag gacgcgacag
     5881 gtataaaaac gtatatttct cttaggttta ataaagtacc tgttttggga aacactcagc
     5941 taggcgttgg aggactttgg agcgacaccc aatattcagt aggtagcagt gatggtttca
     6001 tatactggcc gacaaaggat gaagatgcat acgccctgaa atgtgttggt gattcaatga
     6061 tgcctagaat caaagagggg gagttcgtaa tcgtcgaacc caaccacgac tacactcctg
     6121 gtgatgaagt acttgtggtt acccgggaag gtgaggtcat ggttaaaacc ttcttgttcg
     6181 aacgcgatgg cctgttccat ctaatgtctg taaatgaaga tcatccacca gtcagagtac
     6241 cacgcgaaaa cattgaaaaa atccattacg tggcggggat cgccaaatcc gcgctacgta
     6301 tgtactaacc tttacagtct gataacttac ttctgatcaa accgctctcc acaagggcgg
     6361 ttttcttatt tgcaaaataa ttcattttcg tattgacaca aaatcccata acgcatataa
     6421 atacgatatc gcataattaa tgcgtttttt aatactggct ctttaacaaa ctgaaccgcg
     6481 tgacaggtaa gccgcagtac tcctggcaaa acgaaatagc acccgatggg atcgaggtaa
     6541 gcgccgagtc cgtatgcgta cggtaagcgt agaggacaac accgcgacaa actgataagt
     6601 cacgcaagtt gaaacgcccc gacgatgggg cgcttagttc ctttaactct gggtggccgg
     6661 aagggtaccc atgcttaaag aaattactgg ttgtaaggat cgtaatccgt gattgccttc
     6721 cccggatttt catcttcaga aggcagtcgg gcctctaaag catttcggtt gaacagatag
     6781 gaagccctac agtacgaaca aaccatatta acggctcgcc caaggcctgg cttagtaacc
     6841 agaccttccg cttgcaatat agattttctc ttttgctcgt agcaattggg acaaatataa
     6901 accggagatt ggtcagcatc tgcagaaagc ttgctgcgat agaccagagt accccgcata
     6961 ggctggtaaa gttcatagct cgccgcttca gcggcccagt tctcttgctt tactttctca
     7021 ttttccagtt caacaatttt ctccttagcc gcaatcagca gctcctgaag cgacatttgc
     7081 tgcatctgaa catccattag cttatcgagt aagatgtgcg tcttctcttt aactgcatag
     7141 tcgacagtca gattctgaat ctctcttgct gctccaaccg cgcttttaat tgctcctccc
     7201 acgccggata acgattcagt tatccgggaa agaattcctt tttcttcaga catatctaat
     7261 ctctctcaaa ctgtaggggt gaagagatat tagacgattt ctcactgtag gggtacaagg
     7321 agaactacct cgcctgatgt ggttaaaagc aggcactcga tgatatccat gtaattgctg
     7381 tgtgtagtct ttgcccgcct caagtgacgg gcttttttat gtctgaaagc gcactcgcaa
     7441 cagcgtgctc cccgatatga aaaaaggaat acaaccgatg aaacctgaac acatccatcg
     7501 actgacgggg cgcgatgttc tccgttatcg ccgtaaaaac ttcgatttga tgaccggtct
     7561 ggccgttgcc actgcgctcg gtctgatcat cactttcatt ctccttgtag cgaggaccac
     7621 agtatgagtt tagaaaccag tctcgaactt aataatcaac ttctagcaca acataatgcg
     7681 ctgcttgaac gtcttatccg cacaatggca tcaggtattg ttatgcatcc tgacacgatt
     7741 tcacgagtgc aggaatgtcg ggatacagca actgaagctg aaaacatgtc agcggcaatg
     7801 acactggatg atctggagtt cagcgacgtt atcgcactgg ctgttttcta cccggtagcc
     7861 acccctatca cagaagacat gctgcaacgt gctgttgcct accgtgatgc tgaaggcgat
     7921 aaacgagtag ttcagattga tgctctcgac agcgcattac agggcgtcaa acgagccagg
     7981 gcgctgctta aacctgctct cctggacctg tcccgtaaca ttcttaagtt ctgggacgac
     8041 ctgccaacca tcggcgagcg acgtgctttt gccgagcagc tacttgatgc acctgcagat
     8101 gggcgtgatg aagttaagcc gaaaaaggcc agtaacaaag atggagaacg cacggggccg
     8161 ttttacgtca aaaatgtatc cggcacagca gccagtgaac tccatacctt acgcaagttg
     8221 aacgagatgc ttaaaaaagg ccatatcgag atcaaccgtg ttgagtacct tcagctgcag
     8281 gaagaatttg cacgtagaga cgcagcaaat tccagccaga ataatgacgc caaagatgac
     8341 catacaattg atttcgcggc actacgcaaa caagctgaag ggttgatcct ccagttagca
     8401 aaagggggtt accgggcaga agctattgca attctggaaa aacagggagc caggaaactt
     8461 ggtgaagtaa cggatgaaaa tctcgctgaa gtaatcaccc tggctgaaaa agcactggag
     8521 ggttaatcat gccagacgtt catgcacaac tttctccatc atcagcgcat cgatggatgc
     8581 gctgcccagg aagtctggcg ctggaggcca cacaaccgga taaagaaaca acttttgcaa
     8641 tcgagggcac tgcagcgcac gcgcttgctg aaaaagttct acgaaacagg caaagccacc
     8701 cggaacacta cgccggatgc aatgtttcta tgtttctcgg ctcatacccc cttcgcgaaa
     8761 atcctgataa tacatctggc ccacaggtgg atgatgaaat ggtcgaagcc gttggccggt
     8821 atgttgatac ggtctggact cttgcacaga ataatgaact attggttgaa caacgtgttg
     8881 atttctcaca tataacgggg gtggaagaat ctttcggaac tgccgacggc ataatcatcg
     8941 ctggtaacga attacaaatc cacgacctga aatatggcaa aggcgtccgc attgatgcag
     9001 aacaaaatga gcaactacaa ctgtatgctc tgggtgcgct cgaacaattc agcatgctgt
     9061 atgactttga gactgtaaga ttatttattc accagccaag gctcaaccac gtttcagaat
     9121 ggtcattaac cgtacaggaa cttcagtctt tcggtgaacg ggcacaggag gccgcaacca
     9181 gtgcgatcct tgttctcaat attgctgaat gcgaaggcat tgagacacta ccgctggaaa
     9241 acttcatacc tggagaaaaa cagtgccgct tctgtaaagc aaaagctatt tgcactgccc
     9301 agaaaatgca gcatttacaa acagcggcca gcgactttga agatctgaca aagcctgtca
     9361 gcgaaataat caccaatgcc agcgcacgtg tacctctgtt aaccattgag cagcttgcgg
     9421 agatctatag ccaggccgac tttattgaat cctggctaaa ggcagtacgg gaccgggttc
     9481 acaatgaact caatgccgga catccggtac cggggtttaa actggtaaca ggaaaacaag
     9541 gtaaccgggc ctggagtgat gaagaggcag ctcgcgcact tctgaaggac cagttcaggt
     9601 acaaaactga agaagtattc gactttaaac ttattagtcc cacaaaagcc gaaaaactta
     9661 tcaaaaaggc cagtccgcgc cgttggtcaa aagtcgaggc actgataaca cgagctgatg
     9721 gtaaacccac cgtcgttccc gagtcagacc cacgccccgc actcaatatc aaccctgtaa
     9781 atgatttcga cgacgtatcc gacgatacgc taaccgcaga cctcatctga tttaaggaaa
     9841 tacccatgaa actgaagctg aacaatgttc gtctggcctt cccgtctctg tttgaagcta
     9901 aaactgtaaa cggcgaaggc gatccacgtt tctccgcagt atttttaatg tcacccaaac
     9961 acccacaact ggaagaaatc cgtaaagcta tgaagcaggt agcgaaggaa aaatggggag
    10021 agaagtggga gcccatttat aaccagctgg agaaaaaact caatctgtgc ctgcatgatg
    10081 gtgatgaaaa agcagagtat gaaggcttcc ccggtaattt cttcctgaat gctgctaaca
    10141 aagcgcgccc agctgttctt gatcgcgatc gttcgccact tattcaggct gacggacgtc
    10201 cctatgcagg gtgctatgta aacgccgtta tcgatatctg ggcacaggac aataatttcg
    10261 gtaaacgcat taatgcctca ctcggcggag tccagttcct gcgagacggc gatgcattcg
    10321 ctggcggcgg agtggcaagc gctgacgatt tcgacgatat cagcgaaggt gctgatgctg
    10381 aagcactaat ttaaccctac ttcatagacg cccggttaaa caccgggcta ctgattaggc
    10441 gtataatcac tcaggaatct taatctttct caattcttta tttagttcac agatcttttt
    10501 cttagacggg aaagtatcat acatccaaaa accaacagtc tctaaaacta ttatcataac
    10561 catatacaca agataattta aaaggccttc attttgccct atggaaataa tgcagataat
    10621 agaaaaaagg gtaaacagc
//
LOCUS       DARNVM010000167        10572 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.167, whole genome shotgun sequence.
ACCESSION   DARNVM010000167 DARNVM010000000
VERSION     DARNVM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.167"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..631
                     /locus_tag="SI621_003931"
     CDS             <1..631
                     /locus_tag="SI621_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066589.1"
                     /translation="TLTASLVNELAARMPTEHIYLGQSSGRIGEWISQLPVIGAPFGR
                     YLRSKAAHVHEKPLTPPGNITALVIYLLSCWRAYKFRKMLCKSQQGYLLITDRYPQVE
                     VPGFRFDGPQLAKTTGGNGWIKMLRQRELKLYQWMASYLPVLLIRLGIDEQTAFSRKP
                     DHQLAALQEKIAVTPQLTFNGAKILELDGRQPADEIMQASLRAIHAALS"
     gene            663..1421
                     /locus_tag="SI621_003932"
     CDS             663..1421
                     /locus_tag="SI621_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066590.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKINQF
                     LAQRERQLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGSK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1467..2816
                     /locus_tag="SI621_003933"
     CDS             1467..2816
                     /locus_tag="SI621_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEI4066591.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVFSLIPTLKAFFQRHSLLYNSAGEKS"
     gene            2816..3640
                     /locus_tag="SI621_003934"
     CDS             2816..3640
                     /locus_tag="SI621_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066592.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKSRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            3652..4212
                     /locus_tag="SI621_003935"
     CDS             3652..4212
                     /locus_tag="SI621_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HEI4066593.1"
                     /translation="MSSRLIIALVIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     EDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTDYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4243..5313
                     /gene="lptF"
                     /locus_tag="SI621_003936"
     CDS             4243..5313
                     /gene="lptF"
                     /locus_tag="SI621_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEI4066594.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5310..6389
                     /gene="lptG"
                     /locus_tag="SI621_003937"
     CDS             5310..6389
                     /gene="lptG"
                     /locus_tag="SI621_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEI4066595.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6424..7596)
                     /locus_tag="SI621_003938"
     CDS             complement(6424..7596)
                     /locus_tag="SI621_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEI4066596.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7596..7844)
                     /locus_tag="SI621_003939"
     CDS             complement(7596..7844)
                     /locus_tag="SI621_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEI4066597.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(7876..8790)
                     /locus_tag="SI621_003940"
     CDS             complement(7876..8790)
                     /locus_tag="SI621_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEI4066598.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAADARCGSVRMVGIPLSVLTCLADISTTLSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8787..10517)
                     /locus_tag="SI621_003941"
     CDS             complement(8787..10517)
                     /locus_tag="SI621_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEI4066599.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLIAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNKPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDDIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPDAETRAVSTFVNCGKALP
                     EHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         2534 a         2636 c         2778 g         2624 t
ORIGIN      
        1 gaccctcacg gcaagcctgg taaatgaact ggcagcaaga atgccaacag aacacattta
       61 tctcgggcaa tcgtccgggc gaattggcga atggatttca cagctccctg ttattggcgc
      121 accttttggg cgttatttgc gaagtaaagc ggcacatgtg cacgaaaagc ccttaacacc
      181 gcctggcaat attactgcgc tggttatcta tctgctttcc tgctggcggg cgtacaagtt
      241 tcgcaaaatg ttgtgtaaaa gccagcaagg ctatctgctc atcaccgacc gctacccgca
      301 ggttgaagtg ccgggttttc gctttgatgg cccgcaattg gcaaaaacca cgggcggtaa
      361 cggctggata aaaatgttaa ggcagcgcga gctgaagctg taccaatgga tggcgtctta
      421 tttgcccgta ttgttgattc gtcttggcat tgatgaacaa accgcgtttt cgcgtaaacc
      481 tgaccatcaa ctggcggcgt tacaggaaaa aatcgccgtt acgccgcaac tgacattcaa
      541 tggcgcaaag atccttgaac tggatggtcg gcaaccagcc gatgaaatta tgcaagcgtc
      601 actacgcgca attcacgccg cgctttcctg atcaccaaaa tggatagggg atgatttaac
      661 gaatggatgc actacaaaca caaactgtta atagtacaac cgcgccgcag cccaactaca
      721 ttccggggct gattgcggtg gtcgggtgtg atggcaccgg taaatccacg ctgaccaccg
      781 acctggtgaa atcgctgcaa caacactggc aaaccgagcg ccgctatttg gggctgctct
      841 ccggcgaaga cggcgacaaa atcaaacgat tgccgttggt tggcgtctgg ctggaacggc
      901 gactggcggc caaatcctcg aaaacccaaa gcatgaaaac caaatctcca gcgctatggg
      961 cggcggtgat tatgtactgc ttctcgctgc gaagaatggc gaatctacgc aaggttcagc
     1021 gactggcgca aagtggcgtt ctggtggtca gcgatcgctt cccgcaggct gaaatttcgg
     1081 gcttttatta tgacggaccg gggattggcg tcgagcgcgc gaccgggaaa atcaaccaat
     1141 ttctggcgca gcgcgagcga cagttatacc aacaaatggc gcagtatcgt cctgaattaa
     1201 tcattcgttt ggggattgat attgatactg ccatctcccg caagcctgac catgactatg
     1261 ccgagctgca ggacaaaatc ggcgtcatgt cgacgattgg ctataacggc tcaaaaattc
     1321 ttgagattga ttccagagcg ccctacagcg aagtgctgga acaggcacag aaggcggttt
     1381 ctctggttgc catcgtttct gaccgccgaa gtttaactta aggctggcgg gatacttttt
     1441 actatcaacg ataaggtcag gctgaattgg cgggttttaa catcaaacat tggtttgcag
     1501 atggcgcgtt tcgcaccatt attcgcaata gcgcctggtt aggctccagt aatgtcgtga
     1561 gcgctttgct gggtctgttg gcgctctcgt gtgccggtaa agggatgacg cccgccatgt
     1621 ttggcgtact ggtgattgtg caatcgtacg ccaagtcgat cagcgatttt attaagtttc
     1681 agacatggca gttggtggtg cagtacggaa cgccagcctt gaccaacaat aatccgcagc
     1741 aatttcgcaa tgtcgtctca ttttccttct cgttggatat cgtcagcggc gcggtggcga
     1801 ttgtcggcgg cattgcctta ctcccttttc tttcccattc attaggtctg gatgaccaaa
     1861 gcttttggct ggcagcgctg tattgcacgc tcattccttc aatggcttcc tccacgccga
     1921 ccggcattct gcgtgcggta gatcgcttcg acttaattgc tgtacagcag gcgacgaaac
     1981 cttttctgcg cgcagcgggg agcgtcgttg cctggtattt tgactttggt tttgcgggtt
     2041 ttgttattgc ctggtacgcc tcgaatcttg ttggcggcac catgtactgg tggtttgccg
     2101 cacgtgaatt acgccgcaga aatatccata acgcgttcaa attgaatctg tttgagtctg
     2161 cccgacacat caaaggcgcg tggagttttg tctggtcaac caacattgcc cattccatct
     2221 ggtcggcgcg taattcgtgc agcacagtgt tggtggggat cgtgttagga cccgctgccg
     2281 ccgggttatt taaaatcgcc atgacattct tcgacgccgc cggaacgcca gcgggtttac
     2341 tgggtaaaag tttttaccca gaggtgatgc gtttagatcc gcgcaccacc agaccgtggt
     2401 tgttaggcgt gaagtccggt ttactggcgg gcggaatcgg tattctggtg gcgctcgcgg
     2461 tgttgatcgt cggcaagccg ctcatttcgc tggtgtttgg cgttaagtat ctcgaagcgt
     2521 atgacctgat tcaggtgatg ctgggcgcaa ttgtgatctc gatgctgggc ttccctcagg
     2581 agtcattgct attgatggcg ggaaaacagc gcgcttttct cgtggcgcaa accatcgcct
     2641 caatcggcta catcgtattg ctgttcatgt tctgtcatct gtttggcgtg ctgggggctg
     2701 catttgctta cttcggtggg caatgtctgg atgtggtgtt ctcgctaatt ccgaccttaa
     2761 aggcattttt tcagcgccat tccttgcttt ataactcagc cggagagaaa tcctgatgat
     2821 gagaaaatat ttcccgcttg aggcgagcga gcgtctattt gttgcgattg aagaagatga
     2881 tgttgttgat gcacaggtca gtttgccgcc gactatcgca ctaagttgta ccaccgaaat
     2941 catccatgat aattacgcgt tatgcctgca attctggctc aatggcgtca atcgccagga
     3001 gttactgcgt ttaatatgca aacaggccaa aggcgatgag ctgactgctg atgagcgcaa
     3061 acaatttaaa tacatgcgag cgcgctacaa gcacctgcgt tttgcgcaac ggctgtattt
     3121 aaaaaagcat caggccggat ttttattcgg caaaacgacc gtttttttgg ggcgttttca
     3181 ggacggcttt cgtaacggta agaaaaatat tgtgtcgtat tacggcaacc tgttacgggt
     3241 ttatttaagc tctccggtct ggtcgttggt gaactattca ttacgccata gccagttaga
     3301 aagcgtaagc ggttttattg cctatcggca aaagcaaatg cataccctga aagagataat
     3361 tgccaaatcg cgactaactg gcagggaatt tcatgatgtg cgaaaaatca tcagccagca
     3421 ggtttcttat tacgacaccc taagatcgct tgatcctgaa aataaagagg cgttacagat
     3481 ttcccgcttc ctcgccgcca ttaatggcct gatgggggat aaacacgacg acatggtggc
     3541 ggatgatatg gaaaaccgcc agtcgtatga tgcaccgatg gcgctggata gcgatattcg
     3601 tcagcgctta gaactgctga tttcgcgttt tccgctataa ccggagcaat gatgtcatct
     3661 cgtttgatta ttgcgctggt tatcatgctc ttagccccgg gtgtgcaggc gcacaatttc
     3721 gtcactggta aaacggttac ccctgtttat atccaggaag gcggcgagct gcttctgaat
     3781 agcgaagacg aaattcacta ccaaaagtgg aacagcacac agctggcggg aaaagtccgc
     3841 attattcagt atatcgccgg gcgtaagtcg gcgaagaaaa agaactcact gttaattaaa
     3901 gcggttgaag cggcaaactt tccgcaggat cgttttcagc ccaccaccat tgtgaacact
     3961 gatgatgcca tctttggtac cgactatttt gtcgttggca aaatcgaaaa aaataaacgc
     4021 cgctatccat gggcgcaatt cgttattgac ggcaatggac agggacgcgt tgcctggcac
     4081 ttgcaggagc agagttcaac cattctggtg ctgaataaaa ccgggcaaat tcagtgggca
     4141 aaggacggca gtctgacgcc ggaagaggta gatcatgtca ttgcgctggc gcagaaactg
     4201 atcaatgaat aaaaaattca aaggattgag ggcgttagtg agatgaagct ggttgaacac
     4261 tacatcatgc gtggtacgcg ccgtctggtg ctgattatcg tcggttttct gatctttatt
     4321 ttcgccagct actccgcaca gcgttatctg accgaagcgg caaatgggac attagctctt
     4381 gatgttgtgc tggatatcgt tttctacaag gtgctgattg cactggagat gttgttacct
     4441 gttggactgt atgtgtcagt tggcgtgacg ctagggcaga tgtataccga ctcggaaatt
     4501 accgctatct ctgcggcggg gggcagtccg ggacgcttgt acaaagccgt tctttatctg
     4561 gcgataccgc taagtatttt tgtcaccctt ctgtcgatgt atggtcgtcc gtgggcttat
     4621 gcgcagattt atcaactgga gcaacagtca cagtcggagc tggatgttcg ccagttgcgg
     4681 gcaaagaaat ttaacactaa cgataacgga cgaatgatcc tttcgcagac ggttgatcag
     4741 gataataatc gcctgactga cgcgctgatt tatacttcta ctgccaatcg aacccgcatt
     4801 ttccgcgccc gttcggttga tgtggttgac ccatcacctg agaaaccgac cgttatgttg
     4861 cataacggga ccgcctatct tctcgatcat caggggcgtg acgacaacga acagatctac
     4921 cgtaatctgc aattacatct gaatccgctg gatcaaagcc ctaacgtcaa acgcaaagca
     4981 aaatcggtca cggagctggc gcgctccgtc tttccagccg atcatgccga actgcaatgg
     5041 cgacaaagcc gtggcctgac agcgttgttg atggcgctgc tggccatttc attaagtcgg
     5101 gtaaaaccgc ggcaagggcg attttcaacg ttattgccac tgacgttgct gtttgttgcc
     5161 attttttatg gcggcgacgt ctgccgtacg ctggtggcta acggcgcgat tcccctcatt
     5221 cctggtttgt ggttagtacc cggactcatg ctaatgggcc tgctgatgct ggtcgcacgc
     5281 gacttctctt tgctgcagaa attttcccga tgaatgtttt cagtcgctat ttaatccgtc
     5341 atctctttct cggttttgcc gccgccgcag ggctattgct gccgcttttt actaccttca
     5401 acctgattaa cgaactggat gatgtcagcc cgggcggtta tcgctggact caggcggtgc
     5461 tggtggtgct gatgacctta ccgcgtacac tggtcgaact ttcgccgttt atcgccttat
     5521 tgggagggat tgttggcctg gggcagttat cgaaaaacag tgagcttacc gccattcgca
     5581 gcactgggtt ttctatcttc cgtattgcac tggtggcgct ggttgccggg atattgtgga
     5641 ctgtttcgtt aggcgcgata gatgagtggg tggcgtcgcc attgcagcaa caggcgctgc
     5701 aaatcaaatc gactgccacc gcgttggggg aggacgatga cattaccggc aatatgcttt
     5761 gggccaggcg cggtaatgaa tttgtgacgg tgaaatcgct gaacgagcag ggccagcctg
     5821 tgggcgtgga gatatttcat tatcgcgacg atctctcgct cgaatcctac atttttgcac
     5881 gcagtgcctc cattgaagac gacaaaacat ggatcctgca tggtgtgaat cataaaaaat
     5941 ggttgaatgg caaagaaacg ctggaaacat cagataatct tgcctggcaa tcggccttca
     6001 ccagtatgaa tcttgaagag ttatcgatgc cgggaaatac tttttctgtc cgtcaactta
     6061 atcattacat ccattatttg caggaaactg ggcaacccag cagcgaatac cgccttgcac
     6121 tgtgggaaaa actgggacaa cccatcctga ccctggcgat gattttgctg gctgtgccgt
     6181 ttacctttag tgccccgcgt tcgccaggga tgggtagccg tctcgctgta ggcgtcatcg
     6241 ttggcttact cacctggatc agctatcaaa tcatggtcaa tctggggttg ctatttgcgt
     6301 tgagcgcacc tgttaccgcg ctcggtttac cggtagcgtt tgtgctggtg gcgttgagcc
     6361 tggtgtattg gtatgacaga caacattaaa cccgccgtgg cgcagcgcca cagcgggctc
     6421 acattactgc ttcagagtag cgaacgcttg aatgatggca tcaatttgct cacgactgtg
     6481 ggcagcgttt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gtggcaggat
     6541 aagattcaca taaacgccca gcgaaataag ctcgcgccag atgcgtaaac cgtcctcttt
     6601 cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcgt aacccagttt
     6661 ttgcagaccg tcatacagat ggttggcatt atccatcagc ttttgccgca actctggttg
     6721 tgtagcaatg gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc
     6781 ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc atcgcttccg aaccgacagc
     6841 aaaaccgcca atcgaggcca gacttttact gaaagtacca acgataatat ccacatcatc
     6901 ttcaacgcca acggcttcag ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg
     6961 cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag
     7021 cggcgcaacg tcaccgagca tactgtagat gccttcaacg atgataatcg cctcttttgc
     7081 ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg
     7141 aataatttcc gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc
     7201 cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata
     7261 accggttgaa aaaacgatag cggtagggcg attaaaaaac tcggcgatct ctttctctaa
     7321 cgccagatgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg
     7381 ggccgctaac gctgcctgac cttcggcgat ggcatcatga ttgaaggtta atccgagata
     7441 attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata
     7501 cacttcatca atacaggtac caaacggcgt taacccgttg tcactaaatt gcagtcgctc
     7561 gccagcaaga cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt
     7621 agtgtttcca ttaattgcgc aggggttttg acatccagca gaatattaat aggaatggaa
     7681 atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca
     7741 agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt
     7801 aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac
     7861 ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc taatcccggg
     7921 aaaccaatta atatcttcag aaatacggtt attacttgct gaccagtcgg cgtgggttaa
     7981 ttcacggatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac tcaacgtggt
     8041 actgatatcc gcaaggcagg tgagtaccga cagaggaata ccaaccattc gaacggaacc
     8101 acaacgggcg tcggcggcaa gctgtcttac gcgttgccag tcatagccgc cggcgacgcc
     8161 atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact gccccacggc
     8221 ttgcgcgaaa tcggtgacgt gcaggaaaga gagctgtgta tctggcgcac caagtcgtgg
     8281 cagcaggccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc cgggaccata
     8341 cacggctgtc gggcgaaaaa tgcccagcgt aatttcgtca gccattgcgg tcagtcgttg
     8401 ttcggcgacg tgtttggagt ttgcatacca ggagagttcg ggatgacgcg ccgccagcga
     8461 agagataaac agaaaacgtt ggcaaaagcc gctctctttt gccgcttgca tcaggcgcag
     8521 gctgccgtca acgttacagc gggtaaaaat ctcttctttg tgtccgcgca cttgtccggc
     8581 gcaatggacc accgcgctgg ctccggcaac cagttcgcta agcgaatgtg tatcttccag
     8641 cgaaccgcgc acccagatga gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc
     8701 gcgaacgtga aagccgcggg cgagcaggtt atcgataata tatttaccga taaacccggt
     8761 agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatg aaggcttgca
     8821 gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc ggaggacgtt
     8881 cggggaatac tgtggggcgg caacagatcg atagccgcgg tcacgccaaa ttcgctttgg
     8941 atccgtgctg ccagcgcgtg gataagctgc ccgcgacgtt cttcatcgct gatccgacac
     9001 tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc gccagaatga
     9061 atttccggtt cctgttccgc aatatattca atatcctgcg gccagatatt gcggccacga
     9121 ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag cagataaccg
     9181 aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgtgaaat ctggtcgcca
     9241 aagtaaccgc tcatcaggct agggccggag atgcaaatat ggcctaccac acgttccgcg
     9301 acaggtatac ctgcttcatt gcggatttca atgccatgtt ccggcaacgc tttaccgcag
     9361 ttgacgaaag tcgatacggc gcgtgtttca gcatccggcg cgacggcttt gccctgatat
     9421 tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aagcttcatc agagaagctg
     9481 acagccagcg cattttctgc cagtccgtag cacggcatga acgttttatc gtcaaagtta
     9541 acctgacgaa aacattcagc gaattgatgg agttgttctg cggagatcgg ttctgcgcca
     9601 ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc attcacgcgg
     9661 cgctggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg atttttactg
     9721 atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa ataatctact
     9781 gagagctgcg tggcgacggg ggtcaggaga aagccgacca gtcccatatc atggtagaaa
     9841 ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tgtcatcgtg gcttatagca
     9901 cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa acgggtgctg
     9961 ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag tgcaacatcg
    10021 gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg tttgttatgc
    10081 gtcgcggcat tgaccagcgg taaccactca tcaccagtga taatggctgc gggctggcaa
    10141 cttgccagta aaccctgcag tttggcgctc caggaatccc gctgaccgac gcccattgga
    10201 atcgccaacg ggacggcgat taagccggca tactggcagg aaaaaaaagc ctctacgaac
    10261 ccgctactgg tttcggcaat cagcgccacg cgatcgcctt ttttcaggtt cagcgataac
    10321 aaccgcttcg caccagcttc ggcacgcgct tttaacgtct gatattccag ttgatcttca
    10381 agttggcaac gtctgtcata aaaattcatt ccggcgctac tcagagcggc gtagtccaaa
    10441 gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt aaagatttta
    10501 ttagacatat acaccacgaa aaaattggat atcaaaaata aaatgacaat aatgcggtgg
    10561 attatatagg ca
//
LOCUS       DARNVM010000168        10494 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.168, whole genome shotgun sequence.
ACCESSION   DARNVM010000168 DARNVM010000000
VERSION     DARNVM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.168"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            19..735
                     /locus_tag="SI621_003942"
     CDS             19..735
                     /locus_tag="SI621_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEI4066600.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1190..1265)
                     /locus_tag="SI621_003943"
     tRNA            complement(1190..1265)
                     /locus_tag="SI621_003943"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1230..1232),aa:Asn,seq:gtt)
     gene            complement(1366..3009)
                     /gene="yeeO"
                     /locus_tag="SI621_003944"
     CDS             complement(1366..3009)
                     /gene="yeeO"
                     /locus_tag="SI621_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HEI4066601.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFDGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            3014..3089
                     /locus_tag="SI621_003945"
     tRNA            3014..3089
                     /locus_tag="SI621_003945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3047..3049,aa:Asn,seq:gtt)
     gene            complement(3127..4077)
                     /gene="cbl"
                     /locus_tag="SI621_003946"
     CDS             complement(3127..4077)
                     /gene="cbl"
                     /locus_tag="SI621_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HEI4066602.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4179..5096)
                     /gene="nac"
                     /locus_tag="SI621_003947"
     CDS             complement(4179..5096)
                     /gene="nac"
                     /locus_tag="SI621_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HEI4066603.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5422..5497
                     /locus_tag="SI621_003948"
     tRNA            5422..5497
                     /locus_tag="SI621_003948"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5455..5457,aa:Asn,seq:gtt)
     gene            complement(5553..6488)
                     /gene="ldtA"
                     /locus_tag="SI621_003949"
     CDS             complement(5553..6488)
                     /gene="ldtA"
                     /locus_tag="SI621_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEI4066604.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQLIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(6550..7629)
                     /gene="cobT"
                     /locus_tag="SI621_003950"
     CDS             complement(6550..7629)
                     /gene="cobT"
                     /locus_tag="SI621_003950"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEI4066605.1"
                     /translation="MQTLANLLNTIPVIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(7641..8384)
                     /gene="cobS"
                     /locus_tag="SI621_003951"
     CDS             complement(7641..8384)
                     /gene="cobS"
                     /locus_tag="SI621_003951"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEI4066606.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAISRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(8381..8926)
                     /gene="cobU"
                     /locus_tag="SI621_003952"
     CDS             complement(8381..8926)
                     /gene="cobU"
                     /locus_tag="SI621_003952"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEI4066607.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2969 a         2673 c         2221 g         2631 t
ORIGIN      
        1 ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct
       61 aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgcc
      121 aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca
      181 aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggt
      241 ggagatgaaa cgttcgtaca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt
      301 attgctgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc
      361 aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatacg
      421 ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt gcttgaagct
      481 gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata ttgttattta tactgaacct
      541 actgaccttc ataaaggaat cgcggcgcta aaagcagctg gaatcactga gttctcaaca
      601 acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc
      661 tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt ttatcataac
      721 gtcgcaaatc tctaattata tgttaaagaa atctgtcttt atggcagatt tctttaatct
      781 catataatcc ttataaaaat ataatgttta actcgtcata ttgcttataa aaaatccccg
      841 ttcccagaga aataatattt attaaaatgg caggtcttct ttttctgatt accgaagaca
      901 aagtggcaat tacgtatagt ttcccgataa agacgcgaca gctacatccc gcataaggca
      961 tttttctctt tatctttgta cggtacttca tggaacagaa tttttgacct tgtgaatcgt
     1021 gatgtctgtt ggggaggggc aatttgctca ctgaagcgtg agactcgatt aagcgcacga
     1081 aacacagaaa tcaaaaaccc ggccactttt tttacaaagt gaccgggtaa aaataatttt
     1141 tattttttaa ctgtcctgag actcatagag atgtctcaaa actaaaattt ggctcctctg
     1201 actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca
     1261 gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt
     1321 ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacaac
     1381 tggctttttt tcacactttt gcggctcgga tcgagggtat ttccatagcc aacgtccagt
     1441 aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca
     1501 gacgccaacc acaccccagc caagcatgat tcccagcaca taaccgacta caacccgaca
     1561 accccacatg ctcaacatcg aaacccacat ggcgtaacgg gcatcacgag cacctttaaa
     1621 tccagcgggt agcacccatg aggcggacca aataggcata aataaagcat ttagccaaat
     1681 cagaatcaca acgacgtgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc
     1741 ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggaca gccagaacac
     1801 atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat
     1861 gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgagaa
     1921 cgcgataaaa tttccggcaa taacgctggt ccccatcccg gcaacgaaca tttgggttaa
     1981 taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac
     2041 ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc
     2101 aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat
     2161 ggttaaaccc aacccagccc cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat
     2221 caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca gtagcggtat
     2281 tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg cagctgctgg
     2341 ataactgagt acggtcagct ccagataagt caacgccagt gctttaacgt ctgtcgtggc
     2401 atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg ttgccaacag
     2461 tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg
     2521 atctcgctta ccgagactaa atgctacaac gacagtagta ccaagatcga tagctgcaaa
     2581 aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca tcgcatcttt
     2641 tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac aggcattctc
     2701 catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt tgtagctctt
     2761 gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg agatattcaa
     2821 agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag aatcagcaaa
     2881 gctgcaaaga ttttcgccat caaattgtct gcaaatgcaa caaactgttg atagaaacgg
     2941 caaacagttg ggaaaattaa aaatcgggtt tgacaaaaga tttttcgccg ttaagatgtg
     3001 cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     3061 cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt tatagcgggc
     3121 tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct gccgcttgat
     3181 atcctcaact gaaagtcctg cattacaaag ttccagaaag cgccagacat agttacgctg
     3241 aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat gccgcgtatc
     3301 caggcggatt aaattctttt cctcttgttc accgctggat tgttcggcaa ctaatccgat
     3361 cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac ttaatataat
     3421 atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc ccgtaatccc
     3481 ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg gtgtaatttg
     3541 cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc acgggaaggc
     3601 gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag cttcaccatt
     3661 atgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc gaacctccgg
     3721 gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct gagtatgcgt
     3781 cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa gccgacgaac
     3841 attactggct tcattgagaa tacgttctgc aatgaccagt aatgctttgc ccggttcagt
     3901 catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt catcctccag
     3961 ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg caacctctgt
     4021 caggttgtaa tcctgacgtg cagcttcgcg gattatcttt agttgttgga aattcacggt
     4081 aaactccggg cagttcagat atcccgttat tgttaaagtc taacgcccgg cataacaaat
     4141 aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa ttgccactgc
     4201 cttttttcca tcactggaga actaatcact gacattaaca actctttcac tgcctgtgcc
     4261 tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact catggaaggc
     4321 gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc ggccgattcg
     4381 ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtagc aatagactca
     4441 atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa acgcctcatc aacacgaagt
     4501 ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gtgcaatagc attaacatca
     4561 acgctttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt cagcaaagcc
     4621 tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc gagttggtga
     4681 tttatcagtt tttcgttaag cactgcaccg ctattttcat gaagatagat aacgacctcc
     4741 gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct
     4801 ggcgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
     4861 tgggcctgct cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
     4921 tctgttggcg taacgccccg ttttgtgcga atcaaaagtt gttgatttaa ctcaccttcg
     4981 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctga
     5041 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
     5101 cctccggttt ttgagaatcg gcccaagtgc tgccattact tacaaccaga ttgcaagatg
     5161 cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat aagagattat
     5221 gatagattcg tcattggctc ttttcaccag cagtcgcgtt cccctgccct ataaaaggag
     5281 ggtatgcacc acgatggttc attaccaata agattgaaat ctcatcactt tgttgaaatt
     5341 gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca ctgccattaa
     5401 tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     5461 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc ctgctttcta
     5521 gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga accagaacta
     5581 atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt cacatcaact
     5641 tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac ttttcggtca
     5701 gactcatatt cagcacgatt gcgcgacagc ggctcatgaa cttccagcca ctttgaacca
     5761 tccggttccg ttgtgtattt cactggctga tcaataagtt gcacacgagt acctacagga
     5821 acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc ctgacttacc
     5881 cggagcccaa taccaaaatt ggcattggta ccgtggatgg catatagcct gccaatataa
     5941 atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg caaactctcc
     6001 cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg tgcttcttgt
     6061 ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg gccgatacca
     6121 ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg catctcagcg
     6181 acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg cggaatggtg
     6241 agttgcgaac ctgacttcgg taagaagaca tcagcgcccg gattcgcttc cagcatgtta
     6301 cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt gtgatcagga
     6361 acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa tggataagtt
     6421 accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat atttacacga
     6481 cgcatcatgt ccctttccta agtcgcgaaa gctatcggtt aagtatagct tttatcagac
     6541 ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa ttttactggc
     6601 agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg gcatcgccag
     6661 agctgcgcca ctcccctcac ctaaacgcat atccatattg agaaaaggct ccagccccaa
     6721 atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa tgagatacgg
     6781 tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa gaaatccatc
     6841 cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca ttccgaccaa
     6901 atcaaatcca cccacttttg ccagaacatt aacgccatcc tgcggatttg gctgattcaa
     6961 cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg gcaggtttgc
     7021 gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta ttgctgctgc
     7081 cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac cgttttttgc
     7141 cagctcccgc gtataacata tgacgtccaa aagtaacttt tcagcctgac ggcgactcat
     7201 tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga taagcccggg
     7261 gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc ccgcttgtgc
     7321 tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa tggctgtcac
     7381 ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac acataaccag
     7441 taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg ccagttgtac
     7501 gccaagcgcc tccagccttc ccaagctacc aacaggtttg agtaacccgt caatatgccg
     7561 ttgtgcacgc gacatagcgg caggatcgat gacagggatc gtattcagta aattggcaag
     7621 tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc accaagctct
     7681 atggctgctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag aagctgaccg
     7741 aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat acccggcaat
     7801 aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt
     7861 ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg atacatcaat
     7921 aatgcggcag tcccgcggct gatcgcacat gctgccgcca gcgatgcaag gatcggttcg
     7981 ccacgcagag ccaactcgct taacaccaaa atctttgcca gtacaacaaa aatcaatgcc
     8041 agaccgccgt gggtacctaa acggctatca cgcatgattt ccaacatgcg atcgcggcta
     8101 cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa tcccccggtc
     8161 atcagcacca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc
     8221 agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa aggaaaagta
     8281 ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg cggtacaggc
     8341 agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt tttactccaa
     8401 tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga tttacccgcc
     8461 cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc attcccactt
     8521 cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc aacgactgaa
     8581 tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg tctttatcgc
     8641 cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc aacacaacct
     8701 cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc tccactgtgc
     8761 gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc tcatcatcaa
     8821 ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     8881 ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg actcctgatt
     8941 caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     9001 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt ctcaatccat
     9061 tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt ccgacaaaat
     9121 gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg aattaatgct
     9181 actgccggaa caaccagcaa acgggcattg tgttctgaaa tccaggctat tgattcaacg
     9241 tcagcgacag atctgcgctg taaaagaact tgtaaaacaa cgtaatagaa ttatccggaa
     9301 tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc actgtcacag
     9361 cattcaacag aatgtgactt gccagattta ttagcgccac cagatgttta accgggtaac
     9421 cacgacccag tcggggatat attccatctt ctcgctgaca tcatcaagaa taaaaaggtt
     9481 tatcacactc agagcaacgt tcttcagcag gcgggataat tcttcaacat ttagaagatg
     9541 tgttattaaa ggcatacaaa ctggacgtat tgtattttct tttggtggtt gacctaaggt
     9601 agcagtttat cctgatgcgc tgagatttct gtaatatcta cgtcaacatt ctcctcgaat
     9661 agaaaatgca gccccggcaa gtgattcaca ttttttaaca aaacgttgtt agcgtgccat
     9721 tttcagaagc tctccaagaa agcgaattct tttctcaaat tctgcattct cagacacagc
     9781 tttctactcc atggtaatgg cccaaatacg aagttgctca ggatcattag tggtgctgag
     9841 aagttaaata accatgcaga gtaccactcg tcatcacgta tttatatatc atgaagctat
     9901 tgttgtgttt tgtacatcag tagatatatt ctgatatact ccttttgcta gacataacct
     9961 ttcacctgct tgcaaagctt ctgtgttctg acattgccaa attgttgcaa ttctgtatcc
    10021 agccttcttt cagtcatagc ttcgggccgc gataagactc actgatctga ccctgattcc
    10081 tcttgcagac tttatagacc aattaaaatg cagtttctgc aggtcaacgt ctgaccatca
    10141 ttgtcatcac tctggccatt agagtaacct tctgcattca tccttttgta aaaagtttat
    10201 attagtatca gcaattaacc ggacctgata ctgatatgag tcttaccgca tatacggtca
    10261 atttcagcaa ttaattacat tatccacgcc aaagtatttg tcatcacaat gatggtacct
    10321 tctttcagac accatttttt caactccgtt ttccacggac cgcactctta tgtcaagagt
    10381 gcggtccgtg gatacaacca gagaccgact gacacgagtc agaggaaacg acggatatgt
    10441 tcagtcgtaa aatatctatc aaaaaacatg attaaggtca aaaatgtttg atat
//
LOCUS       DARNVM010000169        10462 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.169, whole genome shotgun sequence.
ACCESSION   DARNVM010000169 DARNVM010000000
VERSION     DARNVM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.169"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            75..2216
                     /locus_tag="SI621_003953"
     CDS             75..2216
                     /locus_tag="SI621_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEI4066608.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFSDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYGDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2280..4172)
                     /gene="parE"
                     /locus_tag="SI621_003954"
     CDS             complement(2280..4172)
                     /gene="parE"
                     /locus_tag="SI621_003954"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEI4066609.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4201..4782)
                     /gene="yqiA"
                     /locus_tag="SI621_003955"
     CDS             complement(4201..4782)
                     /gene="yqiA"
                     /locus_tag="SI621_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HEI4066610.1"
                     /translation="MSMLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4782..5609)
                     /gene="cpdA"
                     /locus_tag="SI621_003956"
     CDS             complement(4782..5609)
                     /gene="cpdA"
                     /locus_tag="SI621_003956"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HEI4066611.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5634..6056)
                     /locus_tag="SI621_003957"
     CDS             complement(5634..6056)
                     /locus_tag="SI621_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HEI4066612.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6057..6686)
                     /gene="nudF"
                     /locus_tag="SI621_003958"
     CDS             complement(6057..6686)
                     /gene="nudF"
                     /locus_tag="SI621_003958"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HEI4066613.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6891..8372
                     /gene="tolC"
                     /locus_tag="SI621_003959"
     CDS             6891..8372
                     /gene="tolC"
                     /locus_tag="SI621_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HEI4066614.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8520..9191
                     /locus_tag="SI621_003960"
     CDS             8520..9191
                     /locus_tag="SI621_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HEI4066615.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9197..10357
                     /locus_tag="SI621_003961"
     CDS             9197..10357
                     /locus_tag="SI621_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEI4066616.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2633 a         2792 c         2577 g         2460 t
ORIGIN      
        1 ggatagtgct tagtgagcaa caagaaacaa ttttcactca cgacaatccg tgagtttttg
       61 ggataacaga ctaaatggct aagttcacac cttcattctc aggaatcaaa ggtcgggcgc
      121 tcttttcact gctctttgca gcaccaatga ttcatgcaac cgacactgca acgaccaaag
      181 atggcgaaac aatcactgtt acagcggatg caaataccgc aactgaggcg accgatggtt
      241 atcaacctct gagcacttcc acggcgacat taaccgatat gccgatgctg gatatcccgc
      301 aggtggtcaa tacggttagc gatcaggttc tggaaaatca gaatgcgacg acgctggatg
      361 aagcgcttta taacgtcagt aacgtggtac agaccaatac attaggcgga actcaggacg
      421 cctttgtacg tcgtgggttt ggtgctaacc gggatggctc catcatgacc aacggcctgc
      481 gaactgtact tcctcgcagt ttcaacgccg ccacagaacg tgtggaagtg ctaaaaggtc
      541 cggcctccac gctgtatggc attctcgatc ctggtggatt gattaacgtc gtgaccaagc
      601 gcccggaaaa aacattccat ggttctgtct cagccacgtc ctccagtttt ggcggaggca
      661 ctgggcaact tgatatcaca ggtcccattg aaggcactca gctggcatac cgcctgacgg
      721 gggaagtgca ggatgaagat tactggcgaa acttcggtaa agagcgcagt acatttattg
      781 ccccgtcact cacctggttt agtgataatg caacagtaac catgctctat tcccatcggg
      841 actataaaac tccgttcgat cgtggaacga ttttcgacct tacgacgaaa cagcccgtaa
      901 acgttgatcg aaaaatacgt tttgacgaac cgtttaatat tacagatggt cagtccgatc
      961 tggcgcaact caacgcagaa tatcatctca atagccagtg gacagcgcgc tttgattaca
     1021 gctacagcca ggataaatac ggcgataatc aggctcgtgt taccgcgtat gatgcaacga
     1081 caggaacgct gacacggcgt gttgatgcaa ctcagggatc tacccagcgt atgcattcta
     1141 ctcgtgcgga tctgcaaggg aatgttgata ttgctgggtt ctataatgag attctgggtg
     1201 gggtgtcata tgaatattat gatcttctgc gcacagatat gattcgctgt aaaaaagcta
     1261 aagatttcaa tatctacaac cctgtttatg gtaataccag caaatgtaca acggtttcgg
     1321 cgtcggacag tgatcagacg atcaaacagg agagctactc agcgtatgca caggacgcgc
     1381 tctatctgac cgataactgg attgccgtcg ccgggatccg ctatcagtat tacacgcagt
     1441 atgcgggtaa aggccgtcct tttaatgtca atactgacag ccgcgatgaa caatggactc
     1501 ccaaactggg gttggtctac aaactgacgc catcggtatc cttatttgct aattattcgc
     1561 aaacatttat gccgcaatcg tcaattgcca gctacattgg cgatcttcca ccggaatcat
     1621 ctaatgctta cgaagtcggg gcaaaattcg agctgttcga tggtataacc gcagatattg
     1681 cgctgtttga tatccataaa cgtaacgtgt tgtataccga aagtattggt gatgaaacca
     1741 tcgccaaaac ggcaggccgc gttcgttcaa gaggggtaga agtcgacctt gcgggagcat
     1801 taactgaaaa cattaatatc attgccagct acggctatac cgatgctaag gttctggaag
     1861 atcctgatta tgcagggaaa ccattgccga atgttcctcg tcataccggt tcgctattcc
     1921 tgacctatga cattcataac atgccaggca ataacacact gacgtttggc ggtggtggac
     1981 atggcgtaag ccgtcgttcg gcaaccaatg gggctgacta ttatctgcca ggctatttcg
     2041 ttgccgatgc cttcgccgca tacaaaatga aattgcagta tccggtcaca ctgcaattaa
     2101 acgtcaaaaa cctgtttgat aaaacgtatt acacctcttc catcgccaca aataatctgg
     2161 ggaatcagat tggcgatccg cgtgaagtgc aattcacggt gaaaatggaa ttttgatcaa
     2221 aaaagcccgg tatacacttg tataccgggc tatgttttat tcttctggcg ggaggattat
     2281 tagacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag
     2341 cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg
     2401 atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc
     2461 gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt
     2521 aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg
     2581 agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg
     2641 cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc
     2701 gcatccgcga ggatacagat tttgccgtaa cgaagctggc tcagatcgtc gctgtcagga
     2761 tcgataccga tcgctaccga aatatcgtgc acttcctgcg aagccagcac ttcgtcggag
     2821 gagacttccc aggtgttaag gatcttacct ttcagtggca tgatagcctg atattcgcga
     2881 tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa cagctcggta
     2941 cggttaaggt cctgcgcggt acaatcagcc agtttgccag gcaacgccgg gccgctggtt
     3001 agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct ggaaatcgcc
     3061 atctctgcca acagctccgc cgcctgaacg ttctggttca gccacaggat gaaggcatct
     3121 ttcaccacgc cagaaacgaa tgccgcacac tgacgcgaag agagacgttc tttggtctgc
     3181 ccggcaaact gcggatcctg catttttact gacagcacat aggcgcagcg atcccagata
     3241 tcttccgccg acagcttcac gccgcgcggc agaatgttgc gatattcaca gaactcgcgc
     3301 atcgcatcca gcaggccctg acgcaggcca ttaacgtgcg taccaccctg catcgttggg
     3361 atcaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc
     3421 cagtccaccg cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc
     3481 ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc
     3541 tgttcggtgt tgttgatctc atctttaaag gtgatctcaa caccagggca cagtaccgct
     3601 ttggctttca gcacatgtgt caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt
     3661 tcatccggcc agaagtgcac gctggtaccg gtattgcgct taccgcaagt gccgacaacc
     3721 tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg
     3781 cgtacgttaa cttctacgcg cttcgacagg gcgttaacta ccgaaatccc cacgccatgc
     3841 aggccgccag agaactggta gtttttgtta gagaatttac cgcctgcatg cagacggcaa
     3901 agaatcagtt caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc
     3961 ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg
     4021 tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg
     4081 cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc
     4141 tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg
     4201 tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt
     4261 gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc
     4321 ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc
     4381 ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata
     4441 ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg
     4501 gcgcaccgcc gggtttacca ccactgcggg cagcataaaa cattgcgaca accaggtggc
     4561 gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac
     4621 aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat
     4681 ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg
     4741 cggcgagctg ttgaaaccgt gtaaataaag aagcatagac atcagtagcc ttctgaagcg
     4801 gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca
     4861 gcatgtaact cgagagtacg ccagccgggc gcgatggtat ccagcgtaaa gttggaacag
     4921 tgcggcttaa actgcacaca ggtcgatggc gttgccaaca ggcggcgacc gttccagtcg
     4981 agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc
     5041 agcacggtat ccagttcgcc cgcgttacgc aggctgtgtt ggtcaagcca actacaaccc
     5101 gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt
     5161 ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc
     5221 tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt cgccggggag
     5281 atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc
     5341 cagacgcagg gcgcacgaaa acttgcaatg ccttcagcga aatgctgata ggccgcagag
     5401 gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctggtgtggc
     5461 cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccccaa cagggcttcg
     5521 tgcttttgcg caaacaggtg agtgtcggta atttgtaaaa tcctgactct ggcctcacca
     5581 gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctaataa
     5641 accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactga
     5701 ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct
     5761 ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga
     5821 cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc
     5881 accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc
     5941 tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa
     6001 aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat
     6061 gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac
     6121 gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca
     6181 tgaacgcgaa tatcttcgtt ttcatcagcc agaccgtgaa taccgcttgc ggtcgtggcg
     6241 tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa
     6301 cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg
     6361 gcaacatctt ccacactttc accctcttca atcatcccgg caaccatctc cagtagccag
     6421 ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct caatcagcac aacttcatca
     6481 cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt
     6541 acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa
     6601 aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta
     6661 acgggcaggt tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg
     6721 tgaatttcag cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta
     6781 acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat
     6841 aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat
     6901 tgctccccat tcttatcggc ctgagcctct ctgggttcag ttcgttgagc caggccgaga
     6961 acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg
     7021 ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc
     7081 taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact
     7141 ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc
     7201 gtgcgttaac gctgcaggaa aaagcagctg ggattcagga cgtcacgtat cagaccgatc
     7261 aacaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg
     7321 ttctttccta tacacaggcg caaaaagaag cgatctaccg tcaattagat caaaccaccc
     7381 aacgttttaa cgtgggcctg gtagcgatca ctgacgtgca gaacgcccgc gcacagtacg
     7441 ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc
     7501 tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc gaaaacttta
     7561 aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt
     7621 cgctgttaca ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg
     7681 atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac acctcatata
     7741 gcggttcgaa aacccgaggt gccgctggta cccagtatga cgatagcaat atgggccaga
     7801 acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg
     7861 tgaaacaggc ccagtacaac tttgttggtg ccagtgagca actggaaagc gcccatcgta
     7921 gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcttctatc agtagcatta
     7981 acgcctacaa acaagccgta gtttccgctc aaagctcatt agatgcgatg gaagcgggct
     8041 attcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg ctgtacaacg
     8101 ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg aatattaagt
     8161 cagccctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac
     8221 cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg
     8281 atggttatgc gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca
     8341 ccagtaacgg tcataaccct ttccgtaact gacgacgacg acggggcttc ggccccgtct
     8401 gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca
     8461 atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa
     8521 tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc
     8581 tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg
     8641 atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa
     8701 gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga
     8761 aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac
     8821 cagcccaggc tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt
     8881 tctggatgcc gctgatggcc ggttacatga tgggtcgtct gatgggcggc ggcgcgggat
     8941 ttgcacagca gccgctgttc tcctcgaaaa acccggccag cccggcttac ggtaaatata
     9001 ccgacgcgac cggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga
     9061 agacggcaat ggcaccaaaa ccggcgacca ctactactgt tacccgtggc ggttttggtg
     9121 aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct tctcgttcaa
     9181 tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgcgaaaa
     9241 agcccacgaa tacggtttca attttcacac catgtacggc gaaccgtact ggtgtgaaga
     9301 tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact
     9361 gcaccagatg tgcctgaaag tggttgaaaa agtgatcgcc agcgatgagc tgatgaccaa
     9421 attccgcatt ccaaaacaca cctggagttt tgtgcgccag tcatggctga ctcaccagcc
     9481 atcgctttat tcgcgtcttg atctggcgtg ggatggtact ggcgagccta aacttctgga
     9541 aaataacgcc gatacgccaa cgtcactgta tgaagccgca ttctttcagt ggatctggct
     9601 ggaagatcag cttaacgccg gtaacctgcc ggaaggcagc gaccagttta acagtctgca
     9661 agagaagctg atcgatcgct ttgtcgagct gcgtgaacag tatggcttcc agttgctgca
     9721 tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg
     9781 cgcgacagaa gctgaaatcg ctactgaatt cctctacatc gacgatatcg gtttaggcga
     9841 aaaaggtcag ttcacggatt tacaggatca ggtgatttct aacctgttca agctgtatcc
     9901 gtgggaattt atgctgcgtg agatgttctc caccaagctg gaggatgcag gcgtacgctg
     9961 gctggaaccg gcgtggaaga gcattatctc caacaaagcg cttctgccgc tactgtggga
    10021 gatgttcccg aatcacccga acctgctgcc tgcttatttt gcggaagatg atcatccgca
    10081 aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat
    10141 tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt
    10201 tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct
    10261 ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcattga tcacccagga
    10321 tatgtctcgg ttttatccgc atatttttgt tgagtaatac tgttcaatat tgccccgttt
    10381 accggggcaa tttattatac ttcgcaaatc atcaccccca tctatatata atcaatcaca
    10441 tccatcactt ttatttaaca tt
//
LOCUS       DARNVM010000170        10407 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.170, whole genome shotgun sequence.
ACCESSION   DARNVM010000170 DARNVM010000000
VERSION     DARNVM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.170"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            175..1569
                     /gene="galP"
                     /locus_tag="SI621_003962"
     CDS             175..1569
                     /gene="galP"
                     /locus_tag="SI621_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HEI4066617.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1646..2143
                     /locus_tag="SI621_003963"
     CDS             1646..2143
                     /locus_tag="SI621_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEI4066618.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKYFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2238..2945
                     /gene="endA"
                     /locus_tag="SI621_003964"
     CDS             2238..2945
                     /gene="endA"
                     /locus_tag="SI621_003964"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HEI4066619.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3025..3756
                     /gene="rsmE"
                     /locus_tag="SI621_003965"
     CDS             3025..3756
                     /gene="rsmE"
                     /locus_tag="SI621_003965"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEI4066620.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3769..4719
                     /gene="gshB"
                     /locus_tag="SI621_003966"
     CDS             3769..4719
                     /gene="gshB"
                     /locus_tag="SI621_003966"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEI4066621.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4828..5391
                     /locus_tag="SI621_003967"
     CDS             4828..5391
                     /locus_tag="SI621_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEI4066622.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5391..5807
                     /gene="ruvX"
                     /locus_tag="SI621_003968"
     CDS             5391..5807
                     /gene="ruvX"
                     /locus_tag="SI621_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEI4066623.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5981..6961)
                     /locus_tag="SI621_003969"
     CDS             complement(5981..6961)
                     /locus_tag="SI621_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEI4066624.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6979..7683
                     /gene="yggS"
                     /locus_tag="SI621_003970"
     CDS             6979..7683
                     /gene="yggS"
                     /locus_tag="SI621_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HEI4066625.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7701..8267
                     /gene="yggT"
                     /locus_tag="SI621_003971"
     CDS             7701..8267
                     /gene="yggT"
                     /locus_tag="SI621_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HEI4066626.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8264..8554
                     /locus_tag="SI621_003972"
     CDS             8264..8554
                     /locus_tag="SI621_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HEI4066627.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8562..9155
                     /locus_tag="SI621_003973"
     CDS             8562..9155
                     /locus_tag="SI621_003973"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEI4066628.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDEQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9148..10284
                     /gene="hemW"
                     /locus_tag="SI621_003974"
     CDS             9148..10284
                     /gene="hemW"
                     /locus_tag="SI621_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEI4066629.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPEQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPNG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLEKAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2458 a         2721 c         2727 g         2501 t
ORIGIN      
        1 agataacagc ctttaatcta tgatgatata actcaattat tttcatgcac ttaaatcata
       61 actaagataa atgttagtgt aagcgattac actgatgtga tttgcttcac atctttttac
      121 gtcgtactca cctatcttaa ttcacaataa aaaataacca tattggaggg catcatgcct
      181 gacgctaaaa aacaggggcg gtcaaacaag gcaatgacgt ttttcgtctg cttccttgcc
      241 gctctggcgg gattactctt tggcctggat atcggtgtaa ttgctggcgc actgccgttt
      301 attgcagatg aattccagat tacttcgcac acgcaagaat gggtcgtaag ctccatgatg
      361 ttcggtgcgg cagtcggtgc ggtgggcagc ggctggctct cctttaaact cgggcgcaaa
      421 aagagcctga tgatcggcgc aatcctgttt gttgccggtt cactgttctc tgcggctgcg
      481 ccaaacgttg aagtactgat tctttcccgc gttctgctgg ggctggcggt gggtgtggcc
      541 tcttataccg caccactgta cctctctgaa attgcgccgg aaaaaattcg cggcagtatg
      601 atctcgatgt atcagttgat gatcactatc gggatcctcg gcgcttatct ttctgatacc
      661 gccttcagct acaccggtgc atggcgctgg atgctgggtg tgattatcat cccggcaatt
      721 ttgctgctga ttggtgtctt cttcctgcca gacagcccac gttggtttgc cgccaaacgc
      781 cgttttgttg atgccgaacg cgtgctgcta cgcctgcgtg acaccagcgc ggaagcgaaa
      841 cgcgaactgg atgaaatccg tgaaagtttg caggttaaac agagtggctg ggcgctattt
      901 aaagagaaca gcaacttccg ccgcgcggtg ttccttggcg tactattgca ggtaatgcag
      961 caattcaccg ggatgaacgt catcatgtat tacgcgccga aaatcttcga actggcgggt
     1021 tataccaaca ccaccgagca aatgtggggg acagtgattg tcggcctgac caacgtactt
     1081 gccaccttta tcgcaatcgg ccttgttgac cgctggggac gtaaaccaac gctaacgctg
     1141 ggcttcctgg tgatggctgc tggtatgggc gtactcggta caatgatgca tatcggtatc
     1201 cactctccgt cggcgcagta tttcgccatc gccatgctgc tgatgtttat tgtcggtttt
     1261 gccatgagtg ccggtccgct gatttgggta ctgtgctccg aaattcagcc gctgaaaggc
     1321 cgcgattttg gcatcacctg ctccactgcc accaactgga ttgccaacat gatcgttggc
     1381 gcaacgttcc tgaccatgct caacacgctg ggtaacgcca acaccttctg ggtgtatgct
     1441 gctctgaacg tactgtttat cctgctgaca ttgtggctgg taccggaaac caaacacgtt
     1501 tcgctggaac atattgaacg taatctgatg aaaggtcgta aactgcgcga aatcggcgct
     1561 cacgattaat ctcctcaggc ttcctcccgt cgtggaggaa gccacctctt gcagtcatct
     1621 tttcttcgct ctatcctctg ccgctatgaa aacatcccgt ctccctatcg ccatccaaca
     1681 ggccgttatg cgtcgcctgc gggaaaaact cgcccaggcc aacctgaagc tagggcgtaa
     1741 ctacccggag ccaaaactct cttacaccca gcgcggaacc tctgccggaa cagcctggct
     1801 ggaaagctat gaaattcgcc tcaatcccgt tttgctgttg gaaaacagtg aagcctttat
     1861 tgaagaagtg gtgccgcacg aactggcaca tttgctggta tggaaatatt tcggccgcgt
     1921 agcgccacat ggcaaagagt ggaagtggat gatggaaagc gtgctgggcg ttcccgcccg
     1981 ccgtacgcat cagttcgaac tgcaatccgt gcgccgcaac accttcccct accgctgcaa
     2041 gtgccaggag catcagctta ccgtacgccg tcataatcgc gtagttcgtg gcgaagccgt
     2101 ctatcgctgt gttcactgcg gcgaacagct ggttgcgaaa taaccacctg aactatcagg
     2161 aactttcctg atctggctga ttgcatacca aaacaacttt cgctacgttg ctggctcgtt
     2221 ttaacacgga gtaagtgatg taccgttatt tgtctattgc tgcggtggta ctaagcgcag
     2281 cattttccgg cccggcgttg gccgaaggta tcaatagttt ttctcaagcg aaagccgcgg
     2341 cggtaaaagt ccacgctgac gcgcccggta cgttttattg cggatgtaaa attaactggc
     2401 agggcaaaaa aggcgttgtt gatctgcaat cgtgcggcta tcaggtgcga aaaaatgaaa
     2461 accgtgccag ccgcgtagag tgggaacacg tcgttcctgc ctggcaattc ggtcaccagc
     2521 gccagtgctg gcaggacggt ggacgtaaaa attgcgccaa agatccggtc tatcgcaaga
     2581 tggaaagcga tatgcataac ctgcagccgt cagtcggtga ggtgaatggc gatcgcggca
     2641 actttatgta cagccagtgg aatggcggtg aaggccagta cggtcaatgc gccatgaagg
     2701 tcgatttcaa agaaaaagtt gccgaaccac cagcgcgtgc acgcggtgcc attgcgcgca
     2761 cctacttcta tatgcgtgac caatacaacc tgacactctc tcgtcagcaa acgcagctgt
     2821 tcaacgcatg ggacaagatg tatccagtta ccgactggga gtgcgagcgt gatgaacgca
     2881 tcgcgaaggt gcagggcaat cataacccgt atgtgcaacg cgcttgccag gcgcgaaaga
     2941 gctaacctac actagcggga ttctttttgt taacccctac cccacgcgta caaccgcgtg
     3001 gggagacgac gcggattttt aactatgcgt atcccccgca tttatcatcc tgaaccactg
     3061 accagccatt ctcacatcgc gctttgcgaa gatgccgcca accatatcgg gcgcgtactg
     3121 cgtatggggc cgggacaggc gctgcaattg tttgacggca gcaaccaggt ctttgacgcc
     3181 gaaattacca gcgccagcaa aaaaagcgtg gaagtgaagg tgctggaagg ccagatcgac
     3241 gatcgcgaat ctccgctgca tattcacctc ggtcaggtga tgtcgcgtgg tgaaaaaatg
     3301 gaatttacta tccagaaatc gatcgaactc ggtgtaagcc tcattacgcc acttttttct
     3361 gagcgctgcg gcgttaaact ggatagtgaa cgtctgaaca agaagctcca gcagtggcag
     3421 aagattgcaa ttgctgcctg tgagcagtgt ggccgtaacc gggtgccgga aatccgtcca
     3481 gcgatggatc tggaagcctg gtgtgcagag caggatgaag gactgaaact gaatcttcac
     3541 ccgcgcgcca gtaacagcat caatacgttg ccgttaccgg tcgaacgcgt ccgcctgctg
     3601 attggcccgg aaggcggttt atcggcagat gaaattgcca tgactgcccg ctatcaattt
     3661 actgatatcc tgttgggacc tcgcgttttg cgtactgaga caactgcgct caccgccatt
     3721 accgcgctac aagtgcgttt tggcgatttg ggctaacgga gaagaataat gatcaagctc
     3781 ggcatcgtga tggaccccat cgcaaacatc aacatcaaga aagattccag tttcgctatg
     3841 ttgctggaag cacagcgtcg tggttacgaa cttcactata tggagatggc cgatctctat
     3901 ttgatcaatg gtgaggctcg cgctcgtacc cgtactttga gcgtcgaaca gaactacgat
     3961 aaatggtacg agttcacggg tgagcaggat ctgccgctgg ccgatctcga tgtgatcctg
     4021 atgcgtaaag acccgccgtt tgataccgag tttatctacg cgacctatat tctggaacgt
     4081 gccgaagaga aagggacgct gatcgttaac aagccgcaga gcctgcgcga ctgtaacgaa
     4141 aaactgttca ctgcctggtt ctctgactta acgccagaaa cactggtcac gcgcaataaa
     4201 gcgcagttga aagcgttctg ggaaaaacac agcgacatca ttcttaagcc gctggacggt
     4261 atgggcggcg cgtcgatttt ccgcgtgaaa gagggtgatc caaacctcgg cgtgattgcc
     4321 gaaaccctga ctgagcatgg cactcgctac tgcatggcgc aaaattacct gccagccatt
     4381 aaagatggcg acaaacgcgt gctggtagtg gatggcgagc cggtgccgta ttgcctggcg
     4441 cgtattccgc aggggggcga aacccgtggc aatctggctg ccggtggtcg cggtgaacct
     4501 cgtccgctga cggaaagtga ctggaaaatc gcccgtcaga ttgggccgac gctgaaagaa
     4561 aaagggctga tttttgttgg tctggatatc atcggcgacc gtctgactga aattaacgtc
     4621 accagcccaa cctgtattcg tgagattgaa gcagagtttc cggtgtcgat caccggaatg
     4681 ttaatggatg ccatcgaagc acgtttacag cagcagtaac ccaccttagc gagaaggatc
     4741 tcgttgagac tctgagtgac agcgcccttc tttccacgca tactgggcgc tgttgctttt
     4801 ttgaaccagg aaacagaacc tctgacaatg aatttacagc atcactttct tattgccatg
     4861 cctgctctcc aggatccgat tttccgtcgt tccgtggtct acatttgcga acataatacc
     4921 aatggtgcaa tggggatcat cgtcaacaag ccgctggaaa atctcaaaat tgaagggatt
     4981 ctggaaaagc tgaagatcac gccggagccg cgtgatgaat caatccgtct ggataaaccg
     5041 gttatgctcg gcggtccgct ggctgaagat cgcgggttta ttttgcatac tccgccctcc
     5101 aattttgctt ccagcattcg catttcagac aacacagtga tgaccacttc ccgcgatgtg
     5161 ctggaaacgc tcggcaccga taaacaaccg tctgacgtat tggtggctct gggttatgcc
     5221 tcctgggaga aaggtcaact ggaacaagaa attctcgata acgcgtggct aacggccccg
     5281 gcagatctga atattctgtt caaaacgccg attgccgacc gctggcgcga ggcggcaaaa
     5341 ctgattggtg tggatattct caccatgcct ggtgtggcag gacacgcctg atgagtggaa
     5401 ccttactcgc cttcgacttc ggcaccaaaa gcattggcgt agcggtcggc caacgcatta
     5461 ccggcaccgc tcgccctttg cctgcaatta aagcgcagga cggtacgccg gactggaaca
     5521 ttatcgagcg tttactgaaa gagtggcagc cggacgaaat tatcgtcggt ttgccgctga
     5581 atatggacgg caccgaacag ccgttaaccg ccagagcgcg taaatttgcc aaccgtattc
     5641 atggtcgttt cggtgttgaa gtaaagctcc atgacgagcg tcttagcacc gtggaggcac
     5701 gttccggtct gtttgaacag ggcggctatc gggcgctcaa caaaggcaaa gtcgactctg
     5761 cctctgcggt tattattctc gaaagctatt tcgagcaggg atattaaggc gatttactag
     5821 cgcacagatt tctgacaatg tgctcgttgt ttcatgttgg atgcggcgta aacgccttat
     5881 ccaacctaca gtgagtgcga attcaaagtt acgcttccct tataggcccg ataagcttgc
     5941 gcatcgggca tatttacaat tcgtatcaga cacagcaacg tcacaagcgc ccttctttca
     6001 cccgctgctg ataactctgc tgaaacgtta tcatccccac ctgctgcccg gtttgaataa
     6061 catgcggtaa ctggtgggtt ttcccttcac gaatcaaatt ccccaccgca ggtgtgttaa
     6121 tcagcaattc aaacaacgcc acgcgtcctt cctgtttatc cacttccagc ttttgtgaca
     6181 acaccgcccg taaactacct gccagttgat tacgcacggg atctttttcc tgcgccggaa
     6241 acgaatccac cagtcgctca actgcctgcg ctgcgccgcg cgtgtgtaat gtcgccagca
     6301 ccagatgccc ggtttctgcc gccgtcagcg ccagacggat tgtctcgcta tcacgcagct
     6361 ctccgagcaa aatcacatca ggatcttccc gcaatgcagc ccgcaatccc gatgcgaaag
     6421 tcatacagtg caaaccaatc tcccgctgtt ggatcaaaca tcgctgactg gtatagagat
     6481 attccacagg atcttccagc gtcagaatat gcgcatcggc atgttgattg agatagccaa
     6541 ccatcgccgc cagcgtggta gatttgccgc tccccgtcgc ccccgtcacc agaatcaggc
     6601 cattctcgct cttgagtaat tccggcaata ccggtggtgc gccaagctgt tcgagctgcg
     6661 ggcagtgcga aggtaacagc cgtaacgcca gagaaatacc ttgccgttgt gcgaacgcac
     6721 tgccgcgcaa tcgctggttt tccgccagcg acacagcaaa atccagctga ccattctcca
     6781 gcaatattgc ccgctgatcg tcatccagcc actcccgcag tagctcttcg acgtccggcg
     6841 cctcaaacgg cgcagcttcc attctcccgc gaatacgcca tcgtgcgggc caggcgctgc
     6901 acaggtgtag atccgagacg ttatgcttta cactaagggc cacaatttct tccatattca
     6961 tactaagatc ctcggaaaat gaacgatatt gcgcataacc tggcacaggt ccgggacaaa
     7021 atctcagcgg ctgcaacgcg ttgcggccgt tctccagaag aaattacgct gcttgcagtc
     7081 agtaaaacaa aacctgcgag cgccatcgca gaagccattg atgccgggca gcgtcaattt
     7141 ggtgaaaact acgttcagga aggggtagat aaaattcgcc actttcagga actgggcgta
     7201 acaggattag aatggcattt tattggcccg ttgcagtcta ataaaagccg cctggtggca
     7261 gagcatttcg actggtgtca taccatcgac cgtttgcgca tcgctacccg cctgaacgac
     7321 cagcgcccgg cagaacttcc ccctcttaac gtactgattc aaattaacat tagtgatgaa
     7381 aacagtaagt ccgggattca actggctgaa ctggacgagc tggcagctgc ggtcgctgaa
     7441 ctaccgcgtt tacgtctgcg cgggctgatg gcaatccctg cgcctgagtc agaatatgta
     7501 aggcagtttg aagttgcacg ccaaatggct gtagcatttg ccggactgaa aacgcgctac
     7561 ccgcatatcg acacgctctc tctgggaatg tcggacgata tggaagccgc cattgcggca
     7621 ggtagcacga tggttcgtat cggcactgca atttttggtg cgcgtgatta ctctaaaaaa
     7681 taaggaatta aaggaacgcc atgaatacgt tgactttcct gctttcaacg gtcattgagc
     7741 tgtataccat ggtgctgtta ttacgcatct ggatgcagtg ggctcattgt gatttttaca
     7801 accccttctc acagtttgtg gtgaaggtga cgcagccgat tatcggacca ctgcgccgcg
     7861 ttattccggc aatgggaccg attgacagcg cctcgctgct ggttgcctat attctcagtt
     7921 ttatcaaagc catcgtgctg tttaaagtgg tgaccttcct gccaatcatt tggattgccg
     7981 gtttattgat tctgctgaaa accatcggcc tgctgatttt ctgggtcctg ctggtgatgg
     8041 cgattatgag ctgggtaagc cagggtcgta gtccgattga atacgtgctg attcagctgg
     8101 ccgatccgct gctgcgcccg attcgccgcc tgctaccggc aatgggtggg attgatttct
     8161 cgccgatgat cctcgttctg ctgctgtatg tcatcaatat gggtgtcgca gaagtattac
     8221 aggcaaccgg aaacatgctg ctgccggggc tgtggatggc gttatgagtg ccgtaacagt
     8281 taacgatgac ggtctggttt tacggcttta tattcagccg aaagccagcc gtgattctat
     8341 tgtcggttta catggcgacg aagtaaaagt cgccattacc gcgccgccgg ttgacggcca
     8401 ggccaacagc catctggtga agtttctcgg taagcaattc cgggttgcca aaagccaggt
     8461 ggtgattgaa aaaggcgaac ttggccgcca caaacaaatt aaaatcatta atccgcaaca
     8521 aatcccgcca gaaatcgcgg cgttaattaa ttaggtatcc tatgcaaaaa gttgtcctcg
     8581 caaccggcaa tgccggtaaa gtgcgtgagc tggcgtcgct gcttagcgac ttcggtcttg
     8641 atatcgtggc ccaaacagac ctcggcgtag attccgctga agaaaccggc ctgaccttta
     8701 ttgaaaacgc gattctgaaa gcgcgccatg cggcaaaagt gaccggttta ccggcaattg
     8761 ccgacgactc cggtctggcg gtagatgcgc tgggtggcgc gccgggtatt tactccgcgc
     8821 gttattccgg tgaagacgcg accgatcaaa agaatctgca aaaactgctg gaaacactga
     8881 aagacgtacc ggacgagcaa cgtcaggcgc gtttccactg cgtgctggta tatctgcgtc
     8941 acgcggaaga tccgactccg ctggtgtgcc acggtagctg gccgggcatg attactcgtg
     9001 aaccagcggg tactggcggc tttggttatg atccaatctt cttcgtacct tccgaaggga
     9061 aaaccgctgc cgaactgacc cgcgaagaaa agagcgccat ttcccaccgt ggtcaggcat
     9121 tgaaactgct gctggacgct ttacgtaatg gttaaattac cgccgctaag tctctacatt
     9181 cacatcccgt ggtgcgtgca gaaatgcccg tactgcgatt tcaactctca cgcgttgaaa
     9241 ggagaagtgc cgcacgacga ttatgttcag catctgctta acgatctgga caacgatgtg
     9301 gcttacgctc agggccgtga agtaaagaca atctttattg gcggtggtac gccgagcctg
     9361 ctttccggcc cggcgatgca aacgctgctg gacggcgtgc gtgcgcgttt gccgctggca
     9421 gcggatgcag aaattacgat ggaagcgaac cctggtacgg tagaagccga tcgctttgtc
     9481 gattatcagc gtgctggtgt gaaccgcatc tctattggtg tgcagagttt tagcgaagaa
     9541 aagctgaaac gacttgggcg cattcatggt ccgcaagaag cgaaacgcgc ggcgaagctg
     9601 gcgagcggtt tagggttacg tagctttaac ctcgatttga tgcacgggct accggagcaa
     9661 tcactggaag aggcgcttgg cgatctgcgc caggccattg aactgaatcc gccgcatctt
     9721 tcctggtatc aactgaccat cgaacccaat acgctgtttg gttcgcgacc accggtgctg
     9781 ccggacgacg atgcgttgtg ggatattttc gaacaggggc atcagttatt aaccgcagcg
     9841 ggttatcagc aatatgaaac ttccgcttac gccaaacccg gttatcagtg ccagcacaat
     9901 ctcaactact ggcgctttgg cgattacatc ggtattggct gcggcgcgca cgggaaagtg
     9961 accttcccga atgggcgcat tctgcgtacc accaaaacgc gtcatccgcg tggttttatg
    10021 caaggaaggt atctggaaag ccagcgtgat gtcgaagccg cagataagcc gtttgagttc
    10081 tttatgaatc gcttccgtct gctggaggcc gcgccgcgcg tggagtttag ccagtatact
    10141 ggcctttcag aagaggttat tcgcccacag ttagaaaagg ctatcgctca gggttatctc
    10201 actgagtgtg cggattactg gcagataacc gagcacggta aattattctt aaactcactt
    10261 cttgaattat tcctcgctga gtaatcatta atgcgcctgc gggcgcatta atcacttctt
    10321 ttaccccccg ttaattaact tcggcaaaaa tatatttctt actgaataaa tatcgacgat
    10381 atttattata tatatttagt gaatatt
//
LOCUS       DARNVM010000171        10236 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.171, whole genome shotgun sequence.
ACCESSION   DARNVM010000171 DARNVM010000000
VERSION     DARNVM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.171"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            126..701
                     /gene="ssuE"
                     /locus_tag="SI621_003975"
     CDS             126..701
                     /gene="ssuE"
                     /locus_tag="SI621_003975"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEI4066630.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPYLLSLRGNAHA"
     gene            694..1653
                     /gene="ssuA"
                     /locus_tag="SI621_003976"
     CDS             694..1653
                     /gene="ssuA"
                     /locus_tag="SI621_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HEI4066631.1"
                     /translation="MRNIIQLALAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEVLNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVPENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPTPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLMPKKVDIRQRIWQPTQLEGKQL"
     gene            1650..2795
                     /gene="ssuD"
                     /locus_tag="SI621_003977"
     CDS             1650..2795
                     /gene="ssuD"
                     /locus_tag="SI621_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEI4066632.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDFYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETSDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANEFIPRKVAQS"
     gene            2807..3598
                     /gene="ssuC"
                     /locus_tag="SI621_003978"
     CDS             2807..3598
                     /gene="ssuC"
                     /locus_tag="SI621_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HEI4066633.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3595..4362
                     /gene="ssuB"
                     /locus_tag="SI621_003979"
     CDS             3595..4362
                     /gene="ssuB"
                     /locus_tag="SI621_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HEI4066634.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGSTPLAQIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4404..7016)
                     /gene="pepN"
                     /locus_tag="SI621_003980"
     CDS             complement(4404..7016)
                     /gene="pepN"
                     /locus_tag="SI621_003980"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEI4066635.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     NGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7282..8484
                     /gene="pncB"
                     /locus_tag="SI621_003981"
     CDS             7282..8484
                     /gene="pncB"
                     /locus_tag="SI621_003981"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEI4066636.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8653..10053
                     /gene="asnS"
                     /locus_tag="SI621_003982"
     CDS             8653..10053
                     /gene="asnS"
                     /locus_tag="SI621_003982"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEI4066637.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2298 a         2666 c         2755 g         2517 t
ORIGIN      
        1 ctttagttta ttttcagaaa aagatacaca aaatttatat ttggcatttt cttgtctctc
       61 cgacagactg accgaattgt tatcaatgtt aacaaaacaa gaacaattgg ttataaggag
      121 agagtatgcg tgtcatcacc ctggcgggta gtcctcgttt tccttctcgc tccagctcct
      181 tgctggaata tgcgcgggaa aaactaaatg gcctggatgt agaggtttat cactggaatc
      241 tgcaaaactt tgccccggaa gatctgcttt atgctcgttt cgatagtccg gcactcaaga
      301 ccttcaccga acagctgcaa caggctgatg gactgatagt cgctacacct gtgtataaag
      361 ccgcctattc cggtgcgctg aaaaccctgc tcgacctgct gccagagcgc gctttgcaag
      421 gcaaagtggt actgccactg gcgacgggcg gcacggtggc ccatctgctg gcggtcgatt
      481 atgcccttaa gccagtttta agtgcgctga aagcccagga gatcctgcac ggcgtgtttg
      541 ccgatgactc acaggtgatt gattaccacc acaagccgca gttcacgcca aatctgcaaa
      601 cccgtcttga taccgcgctg gaaactttct ggcaggcgct gcaccgccgc gatgttcagg
      661 ttccctatct tctgtctctg cgaggtaatg cccatgcgta acatcattca actggcgctg
      721 gcgggattac ttagcgtctc tacgattgcg gttgctgcag aatcctctcc tgaagcgtta
      781 cgtataggct atcagaaagg cagtattggt atggtgctgg caaaaagcca ccagttactg
      841 gaaaaacgct atccgcaaac caaaatctcc tgggtggagt tccccgctgg cccacagatg
      901 ctggaggtgt taaacgttgg cagtattgat ctcggcagta ccggagatat tccgccaatc
      961 tttgcccagg ctgccggggc tgatttggtg tacgtgggcg tcgagccgcc gaagcccaaa
     1021 gccgaagtga ttctggtgcc agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt
     1081 cacaaagttg cctttcagaa aggttccagc tcgcacaatc ttttactacg cgcactacgc
     1141 caggccgggc ttaaattcac ggatatccag cccacttacc tgacgcctgc cgatgcccgc
     1201 gccgcgttcc agcaaggtaa cgtcgacgca tgggctatct gggatcccta ctactccgct
     1261 gcattattac agggcggcgt gcgggtgcta aaagacggta ccgatctcaa tcaaaccgga
     1321 tcgttttatc tggctgcccg tccgtatgca gaaaaaaacg gcgcttttat tcagggcgta
     1381 ctggcaacct ttagtgaggc cgatgcgtta acccgcagcc agcgcgagca aagcatcgct
     1441 ttactggcaa aaacgatggg cttaccaacg ccggtgattg cctcttattt agatcatcgc
     1501 ccccctacca ccatcaaacc ggttaacgcc gagattgccg ccttacagca gcaaacggca
     1561 gatctgtttt atgaaaaccg tctgatgccg aaaaaagtcg atattcgcca gcgtatctgg
     1621 cagcccactc aactggaagg aaaacaatta tgagtctgaa tatgttctgg tttttaccaa
     1681 cccacggtga cgggcattat ctgggaacgg aagaaggttc acgcccggtt gatcacggtt
     1741 atctgcaaca aattgcgcaa gcagcggatc gtctgggcta taccggcgtg ctgatcccaa
     1801 cggggcgctc ctgcgaagat gcgtggctgg ttgccgcgtc gatgatcccg gtgacgcaac
     1861 ggctgaagtt tcttgtcgcc ctgcgtccca gcgtaacctc tcctaccgtt gccgcccgcc
     1921 aggccgccac gcttgaccgt ctctcgaatg gtcgtgcgtt gtttaatctg gtcacaggca
     1981 gcgatccaca agagctggca ggcgacggcg tgttccttga tcatagcgag cgctacgaag
     2041 cctcggcgga atttacccag gtctggcggc gcttattgct gggggaaacc gtcaatttca
     2101 acggtaaaca tattcatgtg cgcggagcca aactactctt cccgccgatt caacagccgt
     2161 atcctccgct ttactttggc ggatcgtcag atgtcgcgca ggagctggcg gcagaacaag
     2221 tagatttcta ccttacctgg ggcgaaccgc cggaactggt taaagagaaa atcgaacaag
     2281 tgcgggcgaa agctgccgcg catggacgca aaattcgttt cggtattcgt ctgcatgtga
     2341 ttgttcgtga aaccagcgac gaagcgtggc aggccgccga gcggttaatc tcgcatcttg
     2401 atgatgagac tatcgccaaa gcacaggccg cattcgcccg gacggattcc gtaggacaac
     2461 agcggatggc ggcgttacat aacggcaagc gcgacaatct ggagatcagc cccaatttat
     2521 gggcgggcgt tggcttagtc cgcggcggtg ccgggacggc gctggtgggc gatggtccga
     2581 cggtcgccgc gcgaatcaac gaatacgctg cacttggcat cgacagtttt gtgctttcgg
     2641 gctacccgca tctggaagaa gcgtatcggg ttggcgagtt gctgttcccg catctggatg
     2701 tcgccatccc ggaaattccc cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcaa
     2761 atgaatttat cccccgtaaa gtcgcgcaaa gctaaggaac aaaatgatgg caacgccagt
     2821 gaaaaagtgg ttattgcgcg ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg
     2881 gcaactggcc tcctcagttg gctggctgtc gacgcgtatt ttgccctcac cggaaggggt
     2941 agtgatggcg ttctggacgc tctccgccag cggcgaattg tggcagcatc tggcgatcag
     3001 ctcctggcgg gcgctcattg gcttttcaat tggcggatcg ctgggcttga ttctgggact
     3061 gattagcggg ctgtcgcgct ggggagaacg gctgctggat acctcaattc agatgttgcg
     3121 caacgtgccg catctggcgc tgatcccgct ggtgattttg tggtttggca ttgatgaatc
     3181 cgcaaaaatc tttctggtgg cgcttggcac gctattcccc atttatatca acacctggca
     3241 tggcatccgt aatatcgatc gcgggctggt ggagatggcg cgtagctatg gattatccgg
     3301 cataccgctg tttatccatg tgatcctgcc tggcgccctg ccctcaatta tggtcggcgt
     3361 gcgttttgcg ttgggcctga tgtggctgac actgatcgtt gccgaaacta tttctgccaa
     3421 ttccggcatt ggttatctgg cgatgaatgc gcgggagttt ctgcaaacgg acgtggtggt
     3481 tgtcgctatt attctttacg ccctgctcgg caaacttgct gacgtcagcg cgcagctgtt
     3541 agagcgcctc tggttgcgct ggaacccggc ttatcatttg aaggaggcca cggtatgaat
     3601 actgctcgtc tgaaccaggg cacaccattg ttgctcaatg cagtaagcaa acattacgca
     3661 gaaaatatcg tcctgaacca actggattta catattccgg caggtcagtt tgtggcggtg
     3721 gtgggccgca gcggtggtgg caaaagtacc ttgctgcgcc tgctggcagg tctggaaacg
     3781 ccaaccgctg gcgatgtgtt agcgggttcc acaccgctgg ctcagattca ggaagacacg
     3841 cgaatgatgt ttcaggatgc gcgtctgctg ccgtggaaat cggtgattga taacgttggg
     3901 ttaggcctta aaggccagtg gcgcgatgcc gcgcgtcggg cgttggctgc agtaggactg
     3961 gagaatcgcg caggggaatg gcctgccgca ctttcaggcg ggcagaaaca gcgagtggcg
     4021 ctggcaagag cgttgattca tcgaccgggt ttattgttgc ttgatgaacc gctcggggcg
     4081 ctggacgcct taacgcgact cgagatgcag gatttgattg tgtcactttg gcaggagcac
     4141 ggctttaccg tactgttggt gacgcatgat gttagcgaag cggtggcaat ggctgaccgg
     4201 gtgttgttaa ttgaagaggg aaaaattggt ctggatttga cggtggatat cccccgtccg
     4261 cgccggttag ggtcggtgcg attagcggag ctggaagcgg aagtgttgca acgggtgatg
     4321 cagcgtggtg aatctgaaac tcgcctgaga aaacaggggt aaattcccga atggcggcgc
     4381 tattgccgcc attcggttat tttttaagcc agtgctttag ttatcttctc gtacagatcg
     4441 ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4501 ttggcatcat aacgtttcag gcgaatcagc ggctcaatca ggcgtgaagc cacctgcggg
     4561 ttacggctgt tgagatcggt tagcatttcc accaggaact ggtaaccgct gccatcttcg
     4621 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc
     4681 gggttgctca tggtaaatga gcgatgctgc aacaggccac gcaccgtctc cagcacattc
     4741 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc atcctgatgc
     4801 cacttgtcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     4861 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     4921 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga
     4981 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     5041 atagccagta gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5101 tcagcaatag caatcgggtc gatgatatcg aacagttctg ccatttcatt gacggaaggc
     5161 agcgtcagga tttctgccgc cagtgccgga tcaatcttct catcaagcag caccgcgcgg
     5221 aaagcgtcag ccacatgcac cggcagagac agcggctgcc cttgctgatg acgcgcgacg
     5281 ttcagcttga tgtaggttgc cagcaggctt tgcgccgcat cccagcggga gaaatcatta
     5341 cgcgcatgac gcatcaggaa ggtcagttgc tgatcgctcc acttatattc cagtttcact
     5401 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaagaca
     5461 aaggtctgtt ccgcctgggt gacgttcagc accgaattga ccggatgacc gcctttctgc
     5521 aacgggatca ctttgccttc gttatcatac agttcgatgg caaacggaat atgcagcggc
     5581 tgtttttctg cctgatccgg cgtagctggc gtgcgctggc tgatggtcag ggtgtactgc
     5641 tcggtttctg gattgtagtc gtctttgacg gtcacaaccg gcgtaccgga ctggctgtac
     5701 caacggcgga aatgagagag atcgacattc gatgcatctt ccatcgcctg cacaaagtcg
     5761 tcacaggtcg ctgcactacc atcatgacgc tcgaaataaa gctgcatccc tttctggaag
     5821 ttttcttcgc ccagcagggt gtggatcatg cgaatcactt ccgcgccctt ctcgtaaacg
     5881 gtcagggtgt agaagttgtt catctcaatg accatatccg ggcggatcgg gtgcgccatc
     5941 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacgttgtt gatacggttt
     6001 accgcgcggg aaccaaggtc agagctgaac tcctgatcgc ggaacacggt taaaccttct
     6061 ttcaggctga gctggaacca gtcgcggcag gtcacacggt taccggtcca gttgtggaaa
     6121 tattcgtgac caataacacg ctcaatatcg aggtaatctt tatcggtggc ggtgtcggta
     6181 cgggccagca catatttgga gttaaagata ttcagacctt tattctccat tgcgcccata
     6241 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt
     6301 tcttcatccc atttcatgga gtttttcagc gaggtcatcg cccacggcgc acgatcaagg
     6361 ttgccgcgat cgacgtacag ctccagcgca acttcgcgac cagaacgcgt ggtgaaggtg
     6421 tcgcgcagca cgtcgaagtc gcctgccacc aacgcaaaca ggtagcacgg tttcgggaac
     6481 gggtcctgcc actgtaccca atgacgtccg ttttccagtt cgccttgcgc aacgcggtta
     6541 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6601 gccagtacgt ccgggcggtc aagataatac gtaatatggc ggaaaccttc ggcttcacac
     6661 tgagtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcggtatt cgtcgccggg
     6721 ctaatttcat taacgatctt aagcgtaaaa cgctccggca aattactgat gaccagtgcg
     6781 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagttta
     6841 agatcttcgc catcaagacg aagcggagca tctgatgcac cattacggac agcctggctg
     6901 accgcggtaa cgaccgtctt ttgcgcgtcc aggtcaaagg tcaagtcaat atcagtaatc
     6961 tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     7021 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7081 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacatg
     7141 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7201 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7261 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7321 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg
     7381 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7441 attcgtgaac aggttcaggc gatgcagcac ctgcgcctgc aggatgatga atatcaatgg
     7501 ctctcagccc tgcctttctt taaggccgac tatcttaact ggctacgcga gttccgcttt
     7561 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg tttaagcggc
     7621 ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta
     7681 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     7741 ttagccgact tctcggcatt aaccgccggt cttgatatgt cgcgcttcca tctgatggat
     7801 tttggcaccc gccgacgttt ttctcgtgaa gtacaagaaa ccatcgtgaa acgtctccaa
     7861 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     7921 ccaatgggaa cacaagcaca cgaatggttc caggcgcatc agcaaatcag cccggatcta
     7981 gccaacagcc agcgagctgc acttgctgcc tggctggaag aatatcccga ccaacttggc
     8041 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc
     8101 gccagtcgct atcagggcct gcgtcatgac tccggcgacc cggttgaatg gggtgaaaaa
     8161 gccattgcac attatgagaa gctgggaatt gatccacaga gtaaaacgct ggttttctct
     8221 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8281 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8341 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8401 ggcaaaacta tctgccacga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8461 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8521 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8581 gtaacatagg tatcccccca tttacgggtg gacaagtgtt tattttttcc gactattaac
     8641 agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg cgtagccgtt
     8701 gacagcgaag tcaccgtgcg cggatgggta cgtacccgcc gagattcaaa agctggcatc
     8761 tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt catcaataat
     8821 tctctgccca attacaatga agacgtcctg cgtctgacca ccggttgctc ggtcattgtg
     8881 acgggtaaag tcgtggcgtc gccgggccag gggcaacaat ttgaaattca ggccagcaag
     8941 gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc aaaacgccac
     9001 agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc
     9061 gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa cgagcaggga
     9121 ttcttctggg tttcaacgcc actgattact gcgtctgata ccgaaggggc tggcgaaatg
     9181 ttccgcgttt ctacgctgga tctggaaaac ctgccgcgta acgatcaggg caaagtggat
     9241 ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca gttgaacggc
     9301 gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt ccgtgctgaa
     9361 aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga agtggcgttt
     9421 gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt cttcaaagcg
     9481 gttctcgaag aacgcgctga cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc
     9541 gtttcacgtc tggagcgctt cattgaagcc gattttgcgc aggtggatta caccgacgca
     9601 gtgaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg gggcgtcgat
     9661 ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc ggtagtggtt
     9721 aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga cggtaaaacc
     9781 gttgcggcta tggacgttct ggctccgggc atcggtgaga tcattggtgg ctcccagcgt
     9841 gaagaacgtc tggacgtgct ggacgagcgt atgctggaaa tgggcctgaa taaagaagat
     9901 tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattcagg tttcggtctt
     9961 ggttttgaac gcctgattgc ttacgtaact ggcgtgcaaa acgtgcgtga tgttattccg
    10021 ttcccacgta ctccgcgtaa cgccagcttc taatttagcg tcttcaagag ccagcgcccg
    10081 ctggctcttt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt
    10141 gcaaccctgc cataacgatc atcctgttac ggaatattac actgcaacat ttgcgcacaa
    10201 aaaataatcc gcattcttat tgcggattag tttttt
//
LOCUS       DARNVM010000172        10149 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.172, whole genome shotgun sequence.
ACCESSION   DARNVM010000172 DARNVM010000000
VERSION     DARNVM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.172"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1309
                     /gene="dcuC"
                     /locus_tag="SI621_003983"
     CDS             <1..1309
                     /gene="dcuC"
                     /locus_tag="SI621_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HEI4066638.1"
                     /translation="NYKPQVVLAAAGIFLMMCGVWLGFGGVLDPAKSSGYLIVDIYNE
                     ILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYIILSATYV
                     IGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIF
                     AAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKALDN
                     VPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEFFRKHNLR
                     ETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVM
                     IIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSP
                     ITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1353..1850)
                     /gene="sspB"
                     /locus_tag="SI621_003984"
     CDS             complement(1353..1850)
                     /gene="sspB"
                     /locus_tag="SI621_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEI4066639.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1856..2494)
                     /gene="sspA"
                     /locus_tag="SI621_003985"
     CDS             complement(1856..2494)
                     /gene="sspA"
                     /locus_tag="SI621_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HEI4066640.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2889..3281)
                     /gene="rpsI"
                     /locus_tag="SI621_003986"
     CDS             complement(2889..3281)
                     /gene="rpsI"
                     /locus_tag="SI621_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEI4066641.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3297..3725)
                     /gene="rplM"
                     /locus_tag="SI621_003987"
     CDS             complement(3297..3725)
                     /gene="rplM"
                     /locus_tag="SI621_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEI4066642.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3944..5071)
                     /gene="zapE"
                     /locus_tag="SI621_003988"
     CDS             complement(3944..5071)
                     /gene="zapE"
                     /locus_tag="SI621_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEI4066643.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQYCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPLMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5265..5663
                     /locus_tag="SI621_003989"
     CDS             5265..5663
                     /locus_tag="SI621_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HEI4066644.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5817..7184
                     /gene="degQ"
                     /locus_tag="SI621_003990"
     CDS             5817..7184
                     /gene="degQ"
                     /locus_tag="SI621_003990"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HEI4066645.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7274..8341
                     /gene="degS"
                     /locus_tag="SI621_003991"
     CDS             7274..8341
                     /gene="degS"
                     /locus_tag="SI621_003991"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HEI4066646.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8404..9342)
                     /gene="mdh"
                     /locus_tag="SI621_003992"
     CDS             complement(8404..9342)
                     /gene="mdh"
                     /locus_tag="SI621_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEI4066647.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEKNALEGMLDTLKKDIALGEEFVNK"
     gene            9777..>10149
                     /gene="argR"
                     /locus_tag="SI621_003993"
     CDS             9777..>10149
                     /gene="argR"
                     /locus_tag="SI621_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HEI4066648.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGT"
BASE COUNT         2477 a         2572 c         2571 g         2529 t
ORIGIN      
        1 aaactacaaa cctcaggtgg tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt
       61 ctggttaggg ttcggtggtg tgctcgatcc cgccaaaagc agcggctact tgatcgtcga
      121 tatttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      181 tatggcagtg ggcggttatg cccgctatat ggagcgcata ggggcaagtc gcgcgatggt
      241 gagcttgtta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      301 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      361 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      421 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      481 tgctgcccag gtcgcaggaa tgaaaattgc cacttacttc ttccactacc agcttccggt
      541 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgctttcga
      601 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaagctctcg ataatgtccc
      661 gccgctctat tacgccattt taccggtgat gccgttaatc ctgatgctcg gctcgctgtt
      721 ccttgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactgag
      781 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      841 tgatgtgcag gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt
      901 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgtcg atgcggttat
      961 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctggt
     1021 cattgccatt tgtgccattg tgatgggctc aggcaatgcg ccgtttatgt catttgccag
     1081 tcttattccg aatatcgcag ccggactaca tgtgccagcg gttgtaatga ttatgccgat
     1141 gcattttgcc acgacgctag cgcgcgctgt ttcgccgatt actgcggtgg tggtcgttac
     1201 gtcaggaatt gcaggcgttt cgccttttgc ggtagtgaag cggacagcga tccccatggc
     1261 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1321 gacaaaacag gccgccaggg cctgttttag tattacttca caacgcgtaa tgctggtcga
     1381 ccaccacgtg gtggctgcgg tggttcatcg tcaggatgag tgtcatcatc gtgatctggc
     1441 ttgtcgccat caataaccga cataacggtt tcgttgtctg ccgatgcctc ttcatcattc
     1501 atgatgctgg catcttcatc gtaggcagct tcaggttcaa acatcgtgcc tgcgccattt
     1561 tcacgggcgt agatagccag cacggcagcc agcggcacag aaacctgacg cggaatgcca
     1621 ccaaagcgcg cattaaagcg cacctcatca ttcgccagtt ccagattgcc gacagctcgc
     1681 ggagcaatgt tgagtacgat ttgcccgtca cgcgcatatt ccataggaac ctgcacgcca
     1741 gggagcgtca catccaccac caggtgcggc gtgagctggt tatccagcaa ccactcatag
     1801 aatgcacgca gcagataggg acgacgtggt gttagctgtg acaaatccat acagattaac
     1861 tccggcccag acgcatttca cgttcagctt cagttaaaga agcaaggaaa gagtcacgct
     1921 caaagacgcg ggtcatatag cctttcagct ctttcgcacc cgggccgctg aactcgatgc
     1981 ccagttgcgg caaacgccac agcagcggag caaggtagca atcgaccagg ctgaactcat
     2041 cgctcaggaa gtacggcttc tgaccgaaga ccggcgcaat cgccagcagt tcttcgcgca
     2101 gttgcttacg tgcggcatct gcttcagaag ctgaaccgtt gatgatggtg ttcatcagcg
     2161 tgtaccagtc tttttcgatg cgatgcatgt acagacggct ttcaccgcga gctaccgggt
     2221 aaacaggcat cagtggcgga tgcgggaaac gctcatccag atattccata atgatgcgag
     2281 attcccacag ggtcagctca cgatccacca gggtcggaac gctctgattc gggttgaggt
     2341 caatcagatc ctgaggcgga ttgtcctttt ccacgtgttc gatctcgaaa cttacacctt
     2401 tctcagccag cacaatgcgg acctgatggc tatagatgtc agtaggaccg gaaaacagcg
     2461 tcattaccga acgtttgttg gcagcgacag ccatgaaaac ctccaggtat agtcagaatt
     2521 tttactgcta ccagccacca ggtggccagt cagaagttgt gttacccagt aaggaacgac
     2581 tctctttgtt cgaaaatcaa acaaaaaatg agcaataccc gacatttggg cagaaaattg
     2641 gatgatagtt taccagattt tgcgaccttt gtggtgagtc gatgccggaa atggggaaaa
     2701 agagatgcgc tttagtctga aatagttgac ttagtccctt attggcgatg tggtttttgt
     2761 tttgcctgtc tgtcagacgg cagcaaaaag caactttcca ttttttacgc tgattcagaa
     2821 tttagccata aaaaaacccg ccgaagcggg ttttttcgca aattgttttc tgccggagca
     2881 gaagccaatt aacgtttgga gaactgcgga cgacgacgtg ctttacgcag accgactttc
     2941 ttacgttcaa cctgacgagc gtcacgagta acgaagccag ctttacgcag ttcagaacgc
     3001 agggactcgt cgtattccat cagagcgcgg gtgataccgt gacggatcgc accagcctga
     3061 ccagagatac caccaccttt aacggtgatg tacaggtcca gtttctcaac catgtcgacc
     3121 agttccagcg gctgacgaac taccatgcgg gcagtttcac gaccgaagta ctgttccaga
     3181 gaacgttggt tgattacgat tttaccgttg cccggtttga tgaaaacgcg agctgcggaa
     3241 cttttgcggc gaccagtgcc gtagtattga ttttcagcca ttgcctataa tcccgattag
     3301 atgtcaagaa cttgcggttg ctgtgccgcg tggttgtgct cgttacccgc gtaaactttc
     3361 agtttacgga acatagcacg acccagcggg ccttttggca acatgccttt aaccgcgatt
     3421 tcaatcacac gctcaggacg gcgagcaatc atctcttcaa aggtcgcttg tttgatacca
     3481 ccgatgtggc cggtgtggtg atagtacact ttgtcagtac gcttgttgcc ggttacagca
     3541 actttgtcag cgttcagaac gatgatgtaa tcaccggtat ctacgtgcgg agtgtattcc
     3601 gctttgtgct taccgcgcag gcgacgagcc agttcagtag ccagacggcc cagagtttta
     3661 ccggtcgcgt caacaacata ccagtcgcgt tttacggttt ctggtttagc tgtaaaagtt
     3721 ttcattaaaa gcttacccaa taaatagtta cacgttggtg aacacccaaa cgtcttcaat
     3781 tgttgaggtt cacacgacaa agtccggcaa acctacccct tcgaatagcc tatgccagca
     3841 cacaaaaagt tttgggaaaa aaactttctt gtaacgtggg gtcgcaggat tatagagaag
     3901 tcggggtcaa agatcgaccc ctttttgtga tttgtggcag gttttaaccc gccaaatgct
     3961 cgcgcttcag atactcttcg ctttgcatct cttgcagacg tgacaggcaa cgctggaact
     4021 caaacttcag ccgatcgccc tgataaattt catacagcgg cacttctgca ctcaccacta
     4081 atttgacatg gcgctcgtaa aactcatcca ccagcgcaat aaagcgccgc gcttcgctct
     4141 ccatcaaccg cgtcataagt ggtacatcaa acaacatgac tgtgtgaaag aggcgtgaga
     4201 gcgcaatata gtcatgctga ctgcgggcgt cgacgcacag cgtagtaaaa gagaccgcca
     4261 gcgtctggtt ctcgacgccc attgtcgcca atggccgatg gttgatttct aacgtcggtg
     4321 aattttctcg tttcgccccc gccagcgcca accatagttt atccatttgc gcccgggttt
     4381 catcgttgag gggcgaaagc cacagatgcg cctgagtgag tgtacgcagt cgataatcaa
     4441 caccagcgtc cacgttcatt acatcacaat actgtttaat ggcatcgatt gcaggcaaaa
     4501 aacgcgcacg ttgtaggcca tttcgataaa gttcgtccgg cggaatattt gacgtcgcta
     4561 ccagggtaat accgcgggcg aacagggctt tcatcagacc gccaagtagc atggcatcgg
     4621 taatatcaga aacaaaaaat tcgtcaaaac agagcacgtc agtttctgct ttaaagcgat
     4681 cggcaataat ttccagcgga tcggtctgcc cctgcaaagc agttagctct tcatgtaccc
     4741 gcagcataaa acggtgaaag tgcagacgct gtttccgctc tcccggcagg ctttgataga
     4801 aaaggtccat cagccaggtt ttcccgcgtc ctacaccgcc ccacatatat aagccacgca
     4861 ctggcgtatg ctttgtgtct tcgcgtttac cccacagttt accgacccgc gccattagcc
     4921 cactcgtcct gggggctgga ggcgtgctat tgatgagttc ctgataaata atttccaggc
     4981 ggctgacggc ctctttttga acgtcgtcgg gttgatggct gccttcattg agcgccttca
     5041 ggtattgcga tgttggggta atgctttgca tgatcttatt gttattcctt gaataatcgg
     5101 tgcgccgttg ttcacggttg acgaaaaaaa ggccgttcta cactacgcga tatgcagtcg
     5161 ggattccact tctgtggaat taacggttat agtggcataa tcagccgcag gcatggagcc
     5221 tgaagccaac accctacgga aacaaaagac aacgggagat gttcatgacc tgggaatatg
     5281 cgctaattgg gttagtcgtc ggcatcatta ttggtgctgt ggccatgcgt tttggtaatc
     5341 gtaaactacg ccagcaacag gcgttgcagt acgaactgga aaagaataaa gctgaactgg
     5401 acgagtatcg cgaagagctg gttagccact ttgcccgcag cgcggaatta ctggatacca
     5461 tggcgcacga ctatcgccag ctgtatcagc atatggcgaa aagctccagc agcctgctgc
     5521 cggaactgtc tgctgaagca aacccgttcc gtaatcgtct ggcagagtct gaagccagca
     5581 acgatcaggc accggtgcag atgcctcgcg actattctga aggtgcatcc ggcctgctgc
     5641 gtactggcgc gaagcgcgac taatttattt ttcgggcgca gccattgcgc cctcctcttc
     5701 tctccctccc cgactatcat ttaatctggt gtctcattgt tagccgtctg aaaattcaat
     5761 aacatcaaac tgttttgaat ctcttttctt atcattcagg tacgagagca ggaataatga
     5821 aaaaacaaac ccagctgttg agtgcattag cgttaagtgt cgggttaact ctctcggcgt
     5881 catttcaggc cgtcgcgtcg attccaggcc aggttgccga tcaggccccc ctccccagtc
     5941 tggctccaat gctggaaaaa gtgcttccgg cagtggtgag cgtacgagtg gaaggaacgg
     6001 ccagtcaggg acagaaaatc ccggaagaat tcaaaaagtt ttttggtgat gatttaccgg
     6061 atcaacctgc acaacccttc gaaggtttag gctccggtgt catcatcaac gccagtaaag
     6121 gctatgtgct gactaacaac catgtgatta atcaggcaca gaaaatcagt attcagctca
     6181 atgatgggcg cgagtttgat gcaaaactga ttggtagcga tgaccagagc gatatcgccc
     6241 tgttgcaaat tcaaaacccg agcaaattaa cgcaaatcgc tattgccgac tccgataaat
     6301 tgcgcgtcgg tgattttgcc gtggcggtcg gtaacccatt tggccttggg caaaccgcca
     6361 cctctggcat tgtgtcagca ttaggccgca gtgggttgaa tcttgaaggt ctggaaaact
     6421 ttatccagac agatgcttcc attaaccgcg gtaactccgg cggtgcactg ttaaatctta
     6481 acggtgagtt aattggcatc aacacggcaa tccttgcgcc tggtggcggg agcgtcggga
     6541 ttggatttgc catccccagt aatatggcgc gaacactggc gcagcagctt atcgactttg
     6601 gtgaaatcaa acgcggtctg ttaggcatca aaggcacaga gatgagtgcc gatatcgcca
     6661 aagccttcaa ccttgacgtg cagcgtggcg cgtttgtcag cgaagtgttg ccaggttctg
     6721 gctcggcgaa agcgggcgtc aaagcgggcg atattattac cagcctcaac ggcaaaccgc
     6781 tgaatagctt tgctgagttg cgctctcgta tcgcaaccac cgagccgggc acgaaagtga
     6841 agcttggcct gctgcgtaac ggcaaaccac tggaagtaga agtgacgctt gataccagca
     6901 cctcttcgtc ggccagcgct gaaatgatca cgccagcgct tgaaggtgca acgttgagcg
     6961 atggtcagct aaaagatggc ggcaaaggta ttaagatcga tgaggttgtc aaaggaagcc
     7021 cagctgctca ggctggcttg caaaaagacg atgtgatcat tggcgtcaac cgcgatcggg
     7081 tgaactcgat tgctgaaatg cgtaaagtgc tggcggcaaa accggccatc atcgccctgc
     7141 aaattgtacg cggcaatgaa agcatctatc tgctgatgcg ttaatgtcgt aaaccgggca
     7201 tcaggctgac gtgtgatgtc cggttaactc gtggtatgct gctgccgttc ccttttttaa
     7261 tgacgcctcc atcatgtttg tgaagctctt acgttccgtt gcgattggat taattgtcgg
     7321 cgctattctg ctggttgcca tgccttcgct gcgcagcctt aacccgcttt ccactccgca
     7381 atttgacagt accgatgaga cgcctgccag ctataatctg gcggttcgcc gcgccgcgcc
     7441 agcggtggtt aacgtttaca accgtggttt gaacaccaac tctcacaacc agcttgagat
     7501 ccgcaccctg ggatccggtg taatcatgga tcaacgcggt tatatcatca ccaataaaca
     7561 cgtcatcaac gacgccgatc agatcatcgt cgccttacag gatggacgtg tatttgaagc
     7621 attgctggtg ggatctgact ctctaaccga tctggcggta cttaaaatta atgccactgg
     7681 cggtttacct accattccaa tcaatgcacg tcgcgtaccg cacattggcg acgtggtact
     7741 ggcgatcggt aacccgtaca acctcgggca gaccattacc caggggatta ttagtgccac
     7801 gggtcgaatc ggtctgaacc cgaccgggcg gcaaaacttc ctgcaaacag atgcttccat
     7861 taaccacggt aactctggcg gcgcgctggt gaactcgctg ggcgaactga tgggcattaa
     7921 cacgctgtcg tttgataaga gtaacgatgg cgaaacgccg gaaggtatcg gctttgcgat
     7981 tcctttccag ttagcaacca aaattatgga taaactgatc cgcgatggtc gcgtgatccg
     8041 cggctacatt ggtatcggcg gacgtgagat cgcaccactg cacgcgcagg gcggtggtat
     8101 agatcaactg caagggatcg tggttaatga agtgtcacct gacggcccgg cggcgaatgc
     8161 gggtattcag gtcaatgatt tgattatttc ggtggataac aaaccggcca tctctgctct
     8221 ggagacgatg gatcaggtgg cggaaattcg ccctggctcg gtgatcccgg ttgtagtgat
     8281 gcgtgatgat aagcagttaa cgctgcaggt caccattcag gaatatccgg caaccaatta
     8341 agtcgtgcgc tcaaaacaaa aaaccggagt ctgtgctccg gttttttatt atccgctaat
     8401 caattactta ttaacgaact cttcgcccag ggcgatatct ttcttcagcg tatccagcat
     8461 accttccagc gcgttctttt caaatgcgct cagagtgccg atagatttac gctcttccac
     8521 gccgttttta cccagcagca gcggttgaga gaagaaacgt gcgtactgac cgtcaccttc
     8581 aacgtaggca cattcgacaa cgccttgttc accttgcagt gcgcgaacca gagacaggcc
     8641 aaaacgtgca gctgcctggc ccatagacag ggttgcagac ccgccaccgg ctttcgcttc
     8701 aaccacctca gtacccgcgt tctggatacg tttggtcaga tcagccactt cctgctcggt
     8761 aaagctaacg ccaggaacct gtgacagcag cggcagaatg gtaacaccag agtgaccgcc
     8821 aataaccggc acttcaactt cgcctggctg tttgcctttc agttccgcaa caaaggtgtt
     8881 ggaacgaatg atatccagcg tggtaacgcc gaacagtttg tttttgtcat aaacaccggc
     8941 ttttttcagc acttcagcag caatcgcaac tgtggtgtta accgggttag tgataatacc
     9001 aatgcacgct ttcgggcagg ttttcgcaac ttgctgtacc aggtttttca cgatgccggc
     9061 gttaacgtta aacaggtcgg aacgatccat acccggttta cgcgctacac ctgcagagat
     9121 aagaacgaca tctgcgcctt ccagcgccgg agtcgcatct tcaccagaaa aacctttgat
     9181 tttcacagca gtagggatat ggctcagatc gacagccaca ccgggagtca ctggagcgat
     9241 atcatacaga gagagttctg aacctgaagg cagttgggtt tttaacagta gtgcaagcgc
     9301 ctggccaata ccgccagcag cgccgaggac tgcgactttc atcctaaact ccttattata
     9361 ttgataaact aagatatgtt gctccgctgc cgcgacctta atccacaaaa ttgccgttta
     9421 caatgaccac atctcaagaa tgtgtagtca cgcaagttta gcgtttatgc atttaattgc
     9481 cgtaatcagg aacctaactt acgtaattaa cagtcgctta tgcattagcg caacattcca
     9541 acaggtggtg acaatatacc ctaccgttca gccaaaacaa catcaatttg ataacaatta
     9601 atttactttt aagcagaatt tgcatgccgt gacgcaggca tgtttctcaa taacgaaatt
     9661 tgataaaatc ccgctctttc ataacattat ttcagccttc ttcagggctg actgtttgca
     9721 taaaaattca tctgtatgca caataatgtt gtatcaacca ccatatcggg tgacttatgc
     9781 gaagctcggc taagcaagaa gaactagtta aagcatttaa agcattactt aaagaagaga
     9841 aatttagctc ccagggcgaa atcgtcgccg cgttgcagga gcaaggcttt gacaatatta
     9901 atcagtctaa agtctcgcgg atgttgacca agtttggtgc tgtacgtaca cgcaatgcca
     9961 aaatggaaat ggtttactgc ctgccagctg aactgggtgt accaactacc tccagtccat
    10021 tgaagaatct ggtgctggat atcgactaca acgatgcagt tgtcgtgatt cataccagcc
    10081 ctggcgcggc gcagttaatt gctcgcctgc tggactcact gggcaaagca gaaggtattc
    10141 tgggcacca
//
LOCUS       DARNVM010000173        10143 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.173, whole genome shotgun sequence.
ACCESSION   DARNVM010000173 DARNVM010000000
VERSION     DARNVM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.173"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(64..681)
                     /locus_tag="SI621_003994"
     CDS             complement(64..681)
                     /locus_tag="SI621_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEI4066649.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISAIAEAVCQLPKLQEVLKRNEII"
     gene            948..2447
                     /gene="ansP"
                     /locus_tag="SI621_003995"
     CDS             948..2447
                     /gene="ansP"
                     /locus_tag="SI621_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HEI4066650.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            complement(2560..3621)
                     /locus_tag="SI621_003996"
     CDS             complement(2560..3621)
                     /locus_tag="SI621_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEI4066651.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILSKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3863..5965
                     /locus_tag="SI621_003997"
     CDS             3863..5965
                     /locus_tag="SI621_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEI4066652.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRVDDASKLSLIFNSVDIKADDPGGLTNAEWKSNPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDISVMMYAGERETTQYQSIPMTPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRDDGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIVWRFE"
     gene            complement(6001..6666)
                     /gene="mcbR"
                     /locus_tag="SI621_003998"
     CDS             complement(6001..6666)
                     /gene="mcbR"
                     /locus_tag="SI621_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HEI4066653.1"
                     /translation="MPGTEKMKHVSLTLQLENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6864..7901)
                     /gene="curA"
                     /locus_tag="SI621_003999"
     CDS             complement(6864..7901)
                     /gene="curA"
                     /locus_tag="SI621_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HEI4066654.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISNGDELVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     VDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8082..8600
                     /gene="mddA"
                     /locus_tag="SI621_004000"
     CDS             8082..8600
                     /gene="mddA"
                     /locus_tag="SI621_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HEI4066655.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIEEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8597..9046
                     /locus_tag="SI621_004001"
     CDS             8597..9046
                     /locus_tag="SI621_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEI4066656.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(9047..9280)
                     /locus_tag="SI621_004002"
     CDS             complement(9047..9280)
                     /locus_tag="SI621_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HEI4066657.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDVELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9366..9539)
                     /gene="ortT"
                     /locus_tag="SI621_004003"
     CDS             complement(9366..9539)
                     /gene="ortT"
                     /locus_tag="SI621_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HEI4066658.1"
                     /translation="MSLYQHMLVFYAVMAAISFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9734..9829
                     /gene="yncL"
                     /locus_tag="SI621_004004"
     CDS             9734..9829
                     /gene="yncL"
                     /locus_tag="SI621_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HEI4066659.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2440 a         2477 c         2490 g         2736 t
ORIGIN      
        1 ttacgtccat cattgtggat aaagttactg aataataaat aacataatct tctggcatcc
       61 cactcaaata atttcattcc tttttaatac ttcttgtaat ttcggtaact gacatacagc
      121 ttcagcaatc gcactgatat tctgcgcatt atcgtgaaac cattcatgcc ccggccccca
      181 tgtgcgcatg gtgcagagat aacagtccac cagcgttagt tgctcgccaa aggcataagg
      241 ttcagcggtc aattgtgaat tcagccagat ataaagcgat ttacgatatt caataacatt
      301 attctttaac tgttcgggtg cgtcaggagc ccaacgctca gggtaatcgg cgaaagtgaa
      361 tgttggatag acattagcaa ccagccagac caatagccgt tgaaactgct gacgttcggc
      421 gcgcccaacc ggtggcgcaa ggtccgggcg acgatcgaga accatcaatg caatcgccgc
      481 tgtctccgtc ataatctcgt cattttccag cgccagtgtc ggaatctgac acaacgggtt
      541 cagggttttc aataactcgc gtgaggttcc ctcgtggtca aaaccactga catcaacgaa
      601 ctgataagga atatcagcca gtgttagcat cagctcactg attgtagagc cccatccggg
      661 tacgccatac accttaatca tatgcccctc acaatctttt aaagattaag tgtaggacag
      721 tacacaggtt aaccagcaaa aggatttgtc attttttcgt cagttgtatt attccccttc
      781 aggattgtaa ggggatgttt tgcatcgttt atcgataacg ggtaggatgc tgcgccgata
      841 gttttatacc cataaagaat aatggtgata actatcatcg ccaggatgaa taaacattgt
      901 tcatggcaac ttatatgact ttttcattaa agcaatcagg gagagcaatg agtaaacacg
      961 acaccgacac ttcagatcaa cacgccgcga aacgccgctg gcttaatgcc cacgaagagg
     1021 ggtatcacaa agcgatgggc aatcgccagg tacagatgat cgccattggc ggcgcgattg
     1081 gtacgggttt gtttttaggc gcgggagccc gactgcaaat ggcgggtcca gcactggcac
     1141 tggtctattt aatttgtggc ttgttttcgt tttttattct gcgtgcattg ggtgagctgg
     1201 tgttacaccg cccttccagt ggcagttttg tctcttatgc ccgtgagttt ttaggagaga
     1261 aagccgctta cgttgcaggc tggatgtact tcatcaactg ggcgatgacg gggattgtcg
     1321 atattaccgc cgttgcactg tatatgcatt actggggcgc atttggtggt gtaccgcagt
     1381 gggtctttgc gctcgctgca cttaccatcg ttggcaccat gaatatgatc ggtgtgaaat
     1441 ggtttgcgga gatggagttc tggtttgcgc ttattaaagt gctcgccatt gtgacctttt
     1501 tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct ggatggcaac accactggct
     1561 ttcatttaat caccgataat ggcggcttct tcccccacgg tttgctgcct gcgctggtgt
     1621 tgattcaggg cgtagtgttt gcttttgcct ccattgaaat ggtgggtaca gctgccggag
     1681 aatgtaaaga tccgcagacc atggtgccta aagcgattaa cagtgtgatt tggcgtattg
     1741 gcctgtttta cgtcggctcc gtggtgttgc tggttatgtt attgccgtgg agcgcgtatc
     1801 aggcggggca aagtccgttc gtgacgtttt tctctaaact gggtgtgcca tatatcggca
     1861 gcattatgaa cattgtggtg ctgaccgctg ccctctccag cctgaattca ggtctgtact
     1921 gcaccggacg tattctgcgc tcaatggcga tgggcggttc cgcaccgagt tttatggcga
     1981 aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc gacactggtt gtgtatgtcg
     2041 tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt tgagattgtg ttgaacttcg
     2101 cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt gtgccagatg cgcctgcgta
     2161 aagcgattaa agaaggcaaa gccgcggatg tcagttttaa actgcctggc gcgcccttca
     2221 cttcctggct gacattactg tttttgctga gcgtcctggt gctgatggcg ttcgattacc
     2281 cgaacgggac ttataccatc gcggcgctgc cgattatcgg tattctgctg gttataggct
     2341 ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac tgcgccagtc gtcgaggaag
     2401 atgaagaaca acaggaaatt gtgtttaagc ctgaaacggc gagttaattg caaaattgcc
     2461 cggctacgtg ccgggcaaat tcaaattact gacgaaggtg ccggatacgg cgtgataaaa
     2521 gtcaggcaag tctctccgtt ttcaccctcg ccatatctat tacagcgcaa tacgaatcac
     2581 atcgtccggc tgggtagctt cctgctgttt agtggatttt tgtttcacac tgacatacag
     2641 cgttttgcca tcggcagaca gcgccaggct gttcggatga gtcggcgtat cgaacgtttt
     2701 caccacttta tagcttttcg cgtcaatcac actgacttta cctgcctgac gatgcgttac
     2761 gtaggcttca ttacgcgccg ggttaaacag cacagccagt gattccggtg ccgcaacctt
     2821 cgacagaata ttgccattac gggtatcgac cactaacact tctgcggctt tagaatcggt
     2881 gataaatgca cgctgcctgg cggtatcaag gctaatgttg ataaagaagt gctctttgcc
     2941 gtcatccagc agctttttac ggctgaggat tttattgtcg gcggtgtcga tggtaatcaa
     3001 ttcgccgtca gcgttagtgg tgtaaagacg tttgcctttg ctatccagcg ccagaccggt
     3061 actcatttta ccggtgttct ggatggcggt tttcagtttg atattctcgc catcaacgac
     3121 ccaaatcacg ctctctttac caataccact gatgtaaacg gtgttcgtgg catcatcagc
     3181 taccagctca cgcggttgca gcgggcgcac ctcttccgtg cgcttacgat catccagcac
     3241 cagacggcct ttcacctcgc ccgttttggc atctatcgcc gtgaccgcgc tgtttacggt
     3301 gttaccaaac cacaacgtct gagtcgtgtt attgatggtg gcaccaaacg gcttaagatc
     3361 gttatggatc gcctgcgtca cttccagagt gaccggatca agacgataaa ccacgccacc
     3421 tttatccagt ttgcggcttt gcgaagtagc aagccacagc gcgttctcct gctggctata
     3481 agccatttcg taggcacctt tacctaccgc tttacgcagc atttcttctg cggcctgcgt
     3541 actgaatgat gaagcaacaa gcaatgaacc taacagtaat gaaccacgca ggcgcgatga
     3601 aaacagatga cgtaaatgca tgacgactcc ctttgatgaa ccgatatttt tgctgcttca
     3661 caatgcttcg ctaagcgtaa tcatcgttac gctcttgtta taaatgggaa tggtaatcat
     3721 tattttctca aatgtggagc atttcttgcc gttacgacta caattttctc aattacttaa
     3781 cgtattgtgc tgtttcgttt tccataaatt tacaatttta ttaccatgac ggcgttttcc
     3841 cattcttaac ctggcttttt tcatgaagat tttttccgtc cgacagaccg tttttcccgc
     3901 gctgcttgtc ctttccccca ccgtttttgc cgctgatgaa cagaccatga ttgtcagtgc
     3961 cgcgccgcag gtggtttcag aactggatac cccagcagcc gtgagcgtgg tggatggcga
     4021 ggagatgcgc ctggcaacac cgcgcattaa cttgtccgaa tcactgacca gcgtgcctgg
     4081 tttgcaggta caaaaccggc agaactatgc gcaagattta cagctgtcga ttcgcggatt
     4141 tggctcccgc tccacttacg gaattcgcgg tattcgcctg tatgttgacg gtattcccgc
     4201 taccatgccc gacggacaag ggcagacatc caatatcgat ttaagcagtg tgcaaaatgt
     4261 ggaagtgctg cgtggcccct tctctgccct gtatggcaac gcgtctggcg gtgtaatgaa
     4321 tgtcaccacc cagaccggac aacagccacc aaccattgaa gccagtagtt attacggcag
     4381 ttttggcagc tggcgctatg ggctgaaagc aacgggcgca acgggagacg gcacacagcc
     4441 cggcgatgtc gattacaccg tctcaaccac gcgttttacg acccacggct atcgtgacca
     4501 tagtggcgca cagaaaaatt tagccaatgc caaactgggc gtacgcgttg atgacgccag
     4561 caaattaagc ctgattttca atagtgtgga tatcaaagca gatgacccag gtgggctaac
     4621 caatgcagaa tggaagtcga atccgcaaca agcgccacgc gctgaacagt acgacacgcg
     4681 aaaaaccatc aagcaaactc aggctgggtt gcgctatgag cgtagcctga gctcgcggga
     4741 tgatattagt gtgatgatgt atgcaggcga gcgagaaacg acccagtacc agtcgatccc
     4801 gatgacacct cagcttaacc cgtcgcatgc gggcggcgta attactctgc aacgccatta
     4861 tcaggggata gacagccgct ggacacaccg tggcgagctg ggcgttccgg tcacgttcac
     4921 tactggcctg aactacgaaa acatgagtga aaaccgcaag ggctacaata acttccgcct
     4981 gaatagcggc gtgccggaat acgggcaaaa aggtgagttg cgccgcgacg aacgcaatct
     5041 gatgtggaac gtcgatccct atttacagac gcagtggcag ctgagcgaaa aactgtcgct
     5101 ggatgctggc gtgcgctaca gctccgtgtg gtttgattcc aacgaccatt acgttactcc
     5161 gggtaacggc gatgacagcg gtgatgccag ttatcacaaa tggctacctg ccggttcgtt
     5221 aaaatatgca atgaccgatg cctggaatat ctatctggca gccgggcgag gttttgaaac
     5281 gccgacgatt aatgagctgt cttatcgtga tgacggtcaa agcggtatga actttggttt
     5341 aaaaccatcc accaacgata caattgagat cggcagtaaa acgcgtattg gtgatgggct
     5401 gcttagtctc gcattgttcc agaccgacac cgatgatgaa attgttgtcg atagcagtag
     5461 cggtgggcgt acgacttaca aaaatgccgg aaagacccgt cgtcaaggcg ctgaactggc
     5521 atgggatcaa cgtttcgcgg gagattttcg cgtaaaagcg tcctggacct ggcttgatgc
     5581 gacctatcgc agcaatgttt gcaatgaaca ggattgtaac ggtaaccgga tgccagggat
     5641 cgcccgtaat atgggctttg cgtcgatagg ttatgtaccg gaagatggct ggtatgcagg
     5701 tacggaagcg cgttacatgg gcgatattat ggcagatgat gaaaatacgg caaaagcgcc
     5761 gtcttatact ctcgtcggct tattcaccgg gtataaatac aattaccaca atttaactgt
     5821 ggatttattt ggtcgtgtcg ataatttatt cgataaagaa tacgttggtt ctgttattgt
     5881 caatgagtca aacgggcgat attacgaacc tgcgcccggg cgaaattatg gtgtcggcat
     5941 gaatattgtg tggcgatttg agtaattacc ttgatgcccg gtatttgccg ggcatttaat
     6001 ttaacgattg tattgctggt atagaatagc aatattttgt tgcataattt cagcaagaca
     6061 atgtctgcat ccctctttgt cttttgcttt taacgcggcg agtaatagac gatagttttc
     6121 tatacgctcc cgtaattccg ctggattaat cgcttcataa agataatgta aaccaggtcc
     6181 catcctgacc cacagttgct caatcatctc acacaggatg ggcatatttg aacgatgata
     6241 aattgctaag cgaaatagcc tgttcgcatt aatgatttgt tccatgtcgc ccttttcttg
     6301 cgcctgctgt aatttctcga gcaattcctc gagttccgcc aggtcttgcg gggtgaggtt
     6361 ttcaacagcc agagtaactg ccattaattc cagctcgtag cggatccgat tgatttcgtc
     6421 taattgacgt ttccccactt ccggaactgt aaatgcttgc gctggtgcga cagaaagcgc
     6481 attcaccgaa accagacgta ataatgcttc acgcacaggt gtaatactca tacctaattg
     6541 ctccgccaga tttttagtaa tcaggcgtgc gccaggtttt agtgcgccaa tactaagctg
     6601 atgtttcagg tcattctcaa gctgcagagt caaactgaca tgtttcattt tttccgttcc
     6661 aggcatttta attccttcgc gtgttttctg tccaaccctg cctgcatggc agaaggagtt
     6721 cacctggggt attaaagtga ataatcataa accactcacc tttattgtta ttagcagcag
     6781 aacaatgacg tggttagtta attaaagctg aaaataagct ttttgcctgg atttttatgg
     6841 cggcttacgc cgccgtcgta tctttaatca tcacccgcca cgcggatcac cactttgccg
     6901 aagtttttac ccttcagcag gccgataaat gtctgtggcg cattctctaa accgtcagta
     6961 atttcttcgc ggtagtggat tttatcctct ttcacccatt gccccatctc cttctgaaac
     7021 tcatggatgc ggtgaccata atcctgagcg ataataaatc cttgcaagcg aatacgtttt
     7081 ttcagcaccg tagccatcag cagaggtaaa cgatccgggc cgggtggtag ctctgtagcg
     7141 ttatagctgc tcactaagcc gcagacggga atgcgcgcag atgtattaag taacggtagc
     7201 accgcatcaa atactttacc gcccacgttt tcataataga tatcaacgcc ttttgggcac
     7261 gctttcgcca gttgttcggc aaaatcatcc gcatggtgat caaggcaaac atcaaaacct
     7321 aacacctcgg tagcatggcg gcatttttcc gcgccaccgg caacccccac caccctgcaa
     7381 cctttgagtt tgccgatttg ccccaccgtt gctcccacag gacctgtcgc cgcagcaacc
     7441 accaatgttt cgccctcttt aggctgaccg atatccagca gccccatata agcggtaaaa
     7501 cctggcatcc ccagcacacc cagcgaccac gatggatttt gcggatgatc gccaagtttc
     7561 accagttcat caccattgga tatgtcatag tcctgccatc cactgtagcc cagcacccag
     7621 tcgccaggct gataatcagg atgattcgac tccacgacac ggctcaccgt accgccgacc
     7681 atcacgccgc caatatcaac aggcggtgaa taagatggct catcgctcat acgtccacgc
     7741 atatacgggt ccagggacaa ataaactgtg cgcagtaaca cctgtccttc acccggtgtg
     7801 gcgacatcat cttcttcaag acgaaaattc tccggaaccg gcgcgccatg cggacgcgag
     7861 gccagaaccc aacgtcgatt acgctgcttt tgttgcccca taatgctctc ctgtgctttt
     7921 ggcatgaatc catcaatagt agcaggctcg caggaatggt tttgtttgca gccgtcacat
     7981 cccctctgca acacgcaata atttcatcga ggaatccgtt gcttcgggct gttttcgata
     8041 gtattgataa cgtcatcatc attattttat cgaggccaga catgtccatc cgttttgccc
     8101 gcaaagccga ctgtgctgcc attgcggaaa tttataacca cgccgtgttg tatacggcgg
     8161 ctatctggaa tgaccaaacg gtggatgctg ataaccgcat tgcctggttt gaagcgcgga
     8221 ccatagcagg ttatccggtg ctggtaagtg aagaagatgg cgtagtgacg ggatatgcct
     8281 cgtttggcga ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat tcggtttatg
     8341 tccatcccga tcatcagggc aaaggtctgg ggcgtaaatt gttaagccga ttgattgagg
     8401 aagcgcggga ttgcgggaag catgtcatgg tcgccgggat cgaatcgcaa aatcaggcct
     8461 cgctgcatct ccaccagtcg ctgggatttg tcgtcaccgc acaaatgccg caggtgggca
     8521 ctaaatttgg gcgctggctg gatctgacat ttatgcagtt gcaactcgac gagcgcactg
     8581 aaccggacgc gattggatga atcagtcgct cacccttgcc tttttgattg ccgccggaat
     8641 tggtctggta gtccagaaca cgctgatggt gcgcatcacc cagacctcct ctaccattct
     8701 catcgccatg ttgctgaact cactggtggg gattgtgctc tttgtttcta ttttatggtt
     8761 taaacaaggt atggcggggt ttggcgaact ggtgtccagc gtgcgctggt ggacacttat
     8821 acctggcttg ctgggatcgt tttttgtctt cgccagtatc agcggatatc agaatgtggg
     8881 agcggcgaca actattgcgg tactggtcgc cagccagctg attggcggtc tggtgctgga
     8941 tatcttccgc agccatggcg taccactacg ggcgttgatc ggcccaattt gtggcgcaat
     9001 tttacttgtg gttggtgcct ggctggtggc caggcgcaca ttttaatcag aggatggtgc
     9061 cacctttggt taactgttcg tggcgcgctt ccatatcttc tttatgtttg cgaccgtgat
     9121 gggcgatcgc tgtgcgcaga cgctgctgct gggtatagcg ttcttcccga cttaactcca
     9181 catcatcgct cagggcgatt aataattcgt tcatatgggc aatgacgctc tcttcaatcg
     9241 cggcatccac gcgcgcgatg acttcatcca gatgcgacat actttctcct tagccacttg
     9301 tgaaatttat cgattcatcc aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt
     9361 aagatttaaa acagtgaaaa caacagcgcc accgggaaac tcatcggcca tgttgccccc
     9421 accagaaagg cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata
     9481 agaaatgaga ttgctgccat aaccgcataa aaaacaagca tatgttgata gagagacatg
     9541 tacgggcgtc cagccagatg aataaaacgc gcgcaaatat gcgtcatttt ttgacgtaca
     9601 tcaagttttt taaccggttt gagaaaaaaa catcaaacag caacttgccc ttatccatct
     9661 tcagaatgag tattattgcg gattttctta acccgtacta tacacatagg ggcaatgata
     9721 aaaggtggca aaaatgaatg tttccagtag aactgtagta ctgataaatg tctttgctgc
     9781 tgttggtttg tttactctta tctctatgag atttggctgg tttatttgat gtagtgattt
     9841 tgcacgcagc acgtactgtc agagaaatca gtgctctgac agtacgcaag atcatctcat
     9901 actctccagg tttacaacat tatccttaat atttacttct ttttgctgac atctacattg
     9961 aaataagtag aaaacatccc cctactgccg tctatacaga cagcttactg attcaaaggg
    10021 aaccttgttt tcacattttg ctatcatcaa tactttagac gcgcgggagt ctataaatgc
    10081 tttatacttt cagggaataa agtaaatata aaaagatggc gtaaattata tgcatggcag
    10141 gat
//
LOCUS       DARNVM010000174        10130 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.174, whole genome shotgun sequence.
ACCESSION   DARNVM010000174 DARNVM010000000
VERSION     DARNVM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.174"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(149..2692)
                     /gene="mdoH"
                     /locus_tag="SI621_004005"
     CDS             complement(149..2692)
                     /gene="mdoH"
                     /locus_tag="SI621_004005"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEI4066660.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDADHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKSWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2685..4220)
                     /gene="mdoG"
                     /locus_tag="SI621_004006"
     CDS             complement(2685..4220)
                     /gene="mdoG"
                     /locus_tag="SI621_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HEI4066661.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYDAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4614..5771
                     /gene="mdoC"
                     /locus_tag="SI621_004007"
     CDS             4614..5771
                     /gene="mdoC"
                     /locus_tag="SI621_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HEI4066662.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTFAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5779..7260)
                     /gene="clsC"
                     /locus_tag="SI621_004008"
     CDS             complement(5779..7260)
                     /gene="clsC"
                     /locus_tag="SI621_004008"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HEI4066663.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7202..7735)
                     /gene="ymdB"
                     /locus_tag="SI621_004009"
     CDS             complement(7202..7735)
                     /gene="ymdB"
                     /locus_tag="SI621_004009"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEI4066664.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENTHLYERLLTQQGDE"
     gene            complement(7830..8141)
                     /locus_tag="SI621_004010"
     CDS             complement(7830..8141)
                     /locus_tag="SI621_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4066665.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8262..8594)
                     /gene="csgC"
                     /locus_tag="SI621_004011"
     CDS             complement(8262..8594)
                     /gene="csgC"
                     /locus_tag="SI621_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HEI4066666.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(8652..9107)
                     /gene="csgA"
                     /locus_tag="SI621_004012"
     CDS             complement(8652..9107)
                     /gene="csgA"
                     /locus_tag="SI621_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HEI4066667.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(9148..9603)
                     /gene="csgB"
                     /locus_tag="SI621_004013"
     CDS             complement(9148..9603)
                     /gene="csgB"
                     /locus_tag="SI621_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HEI4066668.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         2570 a         2588 c         2336 g         2636 t
ORIGIN      
        1 accggcataa cgccggtatt tttattggct cgttgttttt gccagatgtg gcgtgaacgc
       61 cttatccgac ctacaaaatc ttgcaaaatc aacacattgc aggagcgatg taggcctgat
      121 aagcgtagcg catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg
      181 ccagtggatt gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctcagggg
      241 aattccagac gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc
      301 gacgatcgcg attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac
      361 gggcgatttc cagcacctta ctggcgcggt gacgcgcggt cgccattgcg gttgccagag
      421 cgttaaatga cgggttaaac actgcgtgca taaagccatc gtcaagggag cgttggcgat
      481 tcatctcaag gaaccgatcg gtatcaacca gcacctgcgg cggtgaatac tcttccggga
      541 tcaggaacag tttccagctt ttggtgcgca gaccaacggt ggcacggctg gaaatcaccg
      601 aaacaaacgg tgacaggatc aacgagaaga caatcggtgc cagccagaac aggaaacgca
      661 gatccaacca cgccatccca acagcccaca ctaaccctaa cagcagctgt gagccgtggc
      721 gtttgaacgc ttcaccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca
      781 cttcccagcc aaggaacgcg ctgacgacga agaccgtatg gaacagcatg cgtaccggag
      841 ccagcagcac ggaaaacagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc
      901 catattcttt cgttcctttg caccaaataa gcaaaatgct caatagcttt ggcaggaaca
      961 acagcaccat ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccaca
     1021 ccgggaacaa ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgcactacct
     1081 gcaatgcagt agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca
     1141 cgcccgtcag aaataccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga
     1201 agttcatcag gttaccgtgg caccagcggc ggtcacgttt tagctcatca agcaagttag
     1261 gcggtaattc ttcataagaa cccgggagat cgtaagcaat ccagactccc caacctgcac
     1321 ggcgcatcaa cgcagcttcc acgaagtcat gtgacaggat tgaaccggca aaggaacctt
     1381 cgcccggcag cggagccagt gcgcagtgct cgataaacgg tttcacgcgg ataatcgcgt
     1441 tatgtcccca gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg
     1501 gcccatacac gcgagtcgcg aactgctgac agcgtgcata cagcgtatcc ataccggacg
     1561 ctttcggcga cgactgaatg atcccggcgt tcgggttggc ttccatcaaa cgcaccaggc
     1621 cgcataaaca atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact
     1681 ggctgcccca gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcggcgac
     1741 ggcggcggcg atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg
     1801 ctttttgctc tgcgacgcag atatccgggt tataactgtc actcagaatg tagacatcaa
     1861 agtgtttggc attcccggtg gcttttactg attcccacgt tgcacgcaag ccagcaaaaa
     1921 cacggttcac gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta
     1981 atggttcatc gccaactgtt gacgcagata tactgtattt atcgcgacca ataagcagtt
     2041 gcaggaaccc catcaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa
     2101 agaggatcag gataccggtt tgcagcatat aaggcagaag ctgcataaag gaaacccaca
     2161 aatcctgacc aaccatatcc ataggattaa tcagcgccca cccctgataa ggaagaatgg
     2221 tcttcatata ccaggtcgcg acgacagttt gcgcgagcgt caggatcaac agaatgtaac
     2281 ggcggatggt accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac
     2341 gagccagata gcgcggcgtt acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg
     2401 ggttggtacg ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttaa
     2461 gctgatcgcg cccttcgtcg tctttaatta actgtccatc agcaagtgaa tctggccagg
     2521 cttgttccag acgcgccttt accgagcctt gcggggagtc atcctcccgc gcccaggtac
     2581 ggtggtcggc atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt
     2641 tctcgcttgc ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt
     2701 aactggtagc tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt
     2761 tcagtggttt tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta
     2821 accgggttat agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc
     2881 gctgtgaccg gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca
     2941 aaggcgatag taccgtcagg ctggcgaatc aggttcgact gcttcacatc ccccgttgaa
     3001 cgacgcgttt gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg
     3061 aaggtgatgg tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc
     3121 cagtaagcga cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg
     3181 ctgcctttgc cccactcccc tttcggagtc acccatgcgc ttggacgaag atcgtaacga
     3241 tcatcgagat cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc gaagccttgc
     3301 gggttttcca tggagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag
     3361 atccattcgc cattaccagc atggatagag agaccgttag agtcgtgcaa ctccggacga
     3421 tagttatttg caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca
     3481 acccccagtt tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg
     3541 tcacgccctg gcataactac gaatttgtaa gcacctgtcg cgcgcggcga gtcaagcaat
     3601 gcataaatgg ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag
     3661 cgtggaaatt cttcacccga tggcaaggcg gtatcaattg ccaggccgcg tgcagaaagg
     3721 ccataaacct gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt
     3781 tcatcgtttt tatctttgct gttgatcggg taaagcactt taaaaccggc aaaaccaagg
     3841 tcttttaccg tgtctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat
     3901 ttgattcgtt tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac
     3961 ataccctgat ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg
     4021 tcatgattaa actggatctg ctgatagtcc gcgtatttca tgtcgcggaa aacggagggc
     4081 aagttgcttt tgggcgcatc atagcctttc ccggctaagg attgagcttg ctttgcgaca
     4141 tcatcaatac tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc
     4201 aaccaacgca ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt
     4261 tgtgtgctta tatcgatccg atctatttta atggataacc agccattccg acaacagaat
     4321 cccggtatcg ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact
     4381 ttccgtgctt ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaaattta
     4441 tttatcaacc gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat
     4501 aaaaaccttg aagcctgcgc ctggcgcggt caggtcggtg tcgctatggc aacaagccta
     4561 aaggcataac ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaatc
     4621 cagtacccgc gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag
     4681 ggatcccttt tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg
     4741 aaccgtcatt atggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat
     4801 ttttcgttat atccgggtac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat
     4861 ggtggaaagt acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga
     4921 cattgccgca atttattatg ctgcaatacg tcaaagggaa agcggaaagt tggcctggac
     4981 tgtcattgta tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt
     5041 tactggtgtt agtggtcatg acgacgctgt gcgtctggat attcaaacgt atcagaaata
     5101 atttagaaaa ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt
     5161 ttttgtgcct cggcatcggt tatgcggtaa taagaagaac aatttttatt gtgtatccgc
     5221 ccattctgag taacggcatg ttcaatttta ttgtcatgca aacgctattt tatttgccgt
     5281 tctttatcct cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc
     5341 cgtctcgtgg ctgtaccttc gcagcagcat tggcatttgt cgcttattta ctcaaccagc
     5401 gctatggcag tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg
     5461 gtctgtggat ggtgaatgtg gtcttctcct tcggccaccg tttgcttaac ttccagtcag
     5521 cgcgggtgac ttactttgtt aatgcatcgc tgtttatcta tctggttcac cacccgttaa
     5581 cgctgttttt cggcgcgtac attacaccgc acatcacatc caactggctt ggttttctct
     5641 gtggcctgat attcgtagta gggattgcga taattctgta tgaaattcat ttacgcatcc
     5701 cgttactgaa gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac
     5761 cagcccgtta agccgcgttt ataataacca ttccacaggc aatatcgacg ccagtctgac
     5821 cataacccgc ttccagaaac tggtggcggg ttcttttttg agaacaattt ctttctttgc
     5881 atgacgatca acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc
     5941 atactggctc tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac
     6001 gaaccccatt tcagtattga gcaatgtcga acgcggatcg aaattgaaag agccgataaa
     6061 caccgtttta ccatcgatgc taaaggtttt agcatgcagg ctggcaccgg aattaccggt
     6121 tatgccgcga tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc
     6181 cacgccgtag cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac
     6241 atcgttagcg gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg
     6301 taagagttgc gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg
     6361 ttcactgggt gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc
     6421 cttgccctcc cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg
     6481 caacgttcca tcaaccagat gatttataaa cgggctggat tccattttgc gtaaataacg
     6541 atgcgtcatg gcatcgttgt gccaggatgc tggtaactcg atgcgatccg ccatttcacc
     6601 ttccgggaca tccagcacct gctgtagggg tgaaaccgat ttgcaatacc agtagcgggc
     6661 gaaatcatcg gcaacgtcct ctacaactgg tcctattgcc atgacatcta aatccgaaaa
     6721 aagtggctct tcccctgcac caaaataggc atcaccaata tttcgccccc ctaccagcgt
     6781 caccacgcca tcaacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc
     6841 ggtgatataa ccaagcggac gtagcaagcg aaacgagaaa ggattaaaaa gccggacttc
     6901 aatgcgggga tgactgtcaa gcaagcgtaa aatgtcgtca agtccgggtg tattgttgtc
     6961 gtccagcaac aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaagag
     7021 taaccgaccc gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc
     7081 catttcggcc agacgatacc gggcggcaaa cgcatccaga cttttctcca gagggaaaag
     7141 gccacactga ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa
     7201 atcattcatc tccttgttgg gtaaggagtc tttcgtagag gtgggtgttt tcttcatcat
     7261 aacagacaaa atatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt
     7321 ttactgcgat ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag
     7381 gaaatgccac tgacgtatag ctgtttgccg ccaccagtcg cagactattg agataggcat
     7441 cctgcaaaag ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca
     7501 ctacggcttt agcgggaaga tcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat
     7561 cgccctgctg ttgcctgact tttaaacagg catccagcag agccggaccc gctgcgcgat
     7621 gaatggcccc atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca
     7681 catcaacggc cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc
     7741 ctccttacac cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt
     7801 ctgctcagca aggtgagttg ttcagaaact tagttatatt cgatgttcat tacagccagc
     7861 tttttctgtt tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttgacattg
     7921 ccgctcatta acgctttgct ggaataggtt ctgcccggaa cagagccatt ctgtgggcag
     7981 ttcatttcta cgggtgatga ctgggtgctg acgtcacatg ctggttccac aatttggcca
     8041 taaaaatgga tcacgccccc ttgcgcgctg cttcctgcga tggcaataaa aggaaaaaac
     8101 aaactgccga gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg
     8161 attagtcatc cttgagggtt gtgttatcca tactttcagg ttggctgatc cgcagcagac
     8221 cattctctcc agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag
     8281 ggcggccatt gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc
     8341 acccgatcgt ccggggaaat atttaaactc aacttcgtca aagcaatggg ttgattagca
     8401 ggcaatgaga gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg
     8461 gacaatattt gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggtg
     8521 tacatatccc cttgctgggt cgtattaaag gttatctgac tggaaagtgc cgcaaggagt
     8581 aataacgcat tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctttaat
     8641 acagatgtgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtgacg
     8701 ttaacgctgg agttagatgc agtctggtca acagcagcac cgttaccgcc accgaactgt
     8761 ttaacagtca tagtagaatc tttaccattc cactgatcaa gagtagcgct gttgccgaaa
     8821 ccacgctgag tcagatcgat tgagctgtca tcagaacctt ggccaacatc tgcgccgtta
     8881 ccgccgccgt gctgggtaat ggtcagatca gagttacggg cgtcagcttg cagagcaagt
     8941 gcagagttac caccaccgta ctggtaaatg ttcagctctg aatttgggcc gctattatta
     9001 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
     9061 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
     9121 cggattgatt taaaagtcga atagtaatta acgttgtgtc acgcgaattg ccatttgcga
     9181 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
     9241 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
     9301 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
     9361 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
     9421 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
     9481 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
     9541 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
     9601 catgttgtca ccctggacct ggtcgtacac ttaagaaatt aaatcatttc aacttggttg
     9661 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
     9721 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
     9781 acccacgtaa atacgctgtt atctacgcaa aaaaatattt tgttttcttt taaatctccg
     9841 ttttccgcaa tcaaaaatca ccagacagcc attcttcttg cccgtcgctg attgctgcga
     9901 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg
     9961 aggtgtgaag aaaaaacaag aacgttttac atgacgaaag gactacaccg aaatattttt
    10021 tatatgcatt attagtaagt tagcaccatt tgtatgattt tttaaaattg tgcaataaaa
    10081 accaaatgta caacttttct atcatttcta aacttaataa aaccttaagt
//
LOCUS       DARNVM010000175        10045 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.175, whole genome shotgun sequence.
ACCESSION   DARNVM010000175 DARNVM010000000
VERSION     DARNVM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.175"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            508..1485
                     /gene="csiD"
                     /locus_tag="SI621_004014"
     CDS             508..1485
                     /gene="csiD"
                     /locus_tag="SI621_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HEI4066669.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1505..2773
                     /gene="lhgO"
                     /locus_tag="SI621_004015"
     CDS             1505..2773
                     /gene="lhgO"
                     /locus_tag="SI621_004015"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEI4066670.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2796..4244
                     /gene="gabD"
                     /locus_tag="SI621_004016"
     CDS             2796..4244
                     /gene="gabD"
                     /locus_tag="SI621_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEI4066671.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4258..5538
                     /gene="gabT"
                     /locus_tag="SI621_004017"
     CDS             4258..5538
                     /gene="gabT"
                     /locus_tag="SI621_004017"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEI4066672.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREFL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5776..7176
                     /gene="gabP"
                     /locus_tag="SI621_004018"
     CDS             5776..7176
                     /gene="gabP"
                     /locus_tag="SI621_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HEI4066673.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7197..7859
                     /gene="csiR"
                     /locus_tag="SI621_004019"
     CDS             7197..7859
                     /gene="csiR"
                     /locus_tag="SI621_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HEI4066674.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7860..8309)
                     /gene="kbp"
                     /locus_tag="SI621_004020"
     CDS             complement(7860..8309)
                     /gene="kbp"
                     /locus_tag="SI621_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HEI4066675.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8393..8551)
                     /locus_tag="SI621_004021"
     CDS             complement(8393..8551)
                     /locus_tag="SI621_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEI4066676.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8734..9033
                     /locus_tag="SI621_004022"
     CDS             8734..9033
                     /locus_tag="SI621_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4066677.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9043..9567
                     /gene="ygaP"
                     /locus_tag="SI621_004023"
     CDS             9043..9567
                     /gene="ygaP"
                     /locus_tag="SI621_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HEI4066678.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9614..10018)
                     /gene="stpA"
                     /locus_tag="SI621_004024"
     CDS             complement(9614..10018)
                     /gene="stpA"
                     /locus_tag="SI621_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HEI4066679.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2308 a         2571 c         2740 g         2426 t
ORIGIN      
        1 aaaaacatgg ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc
       61 cctggtaaag catttacgct tagaggcgga actctaaata tcaatattcc tgggcgtagc
      121 gctcttcttt tagggaaaac tggagaaccg ccgaactatc tctatttgta atttaatgtt
      181 atatctgccc cgctaaaacg gggcagataa tatgtttagt ttactaacgg tcattttgca
      241 gtgaagccat ttaccgtttt ttatcgacca gataatctgt tctctgatgg taatcctccc
      301 ctaacctgtt gctttagtta ttcatttcct gtctcacttt gccttaatac cctacgttaa
      361 atgttaccaa tttgttgctt ttgatcacaa taagaaaaca atatggcgca tttgtgcgca
      421 tttttcagaa atgtagatat ttttagatta tggctacgaa atgagcatcg ccatgtcacc
      481 ctacatctca taagaggatc gcttctgatg aatgcactga ccgccgtaca aaataacgct
      541 gtcgattcag gccaggacta tagcggattc actctcatcc cgtcggcgca atccccgcgt
      601 ctgctggaac tcaccttcac cgaacagacg accaaacagt ttctcgaaca ggttgccgaa
      661 tggcccgtgc aggcgctgga gtacaaatct tttctgcgtt ttcgggtagg caaaattctt
      721 gacgatctgt gtgcgaacca gctgcaacca ttgctgttga agaccctgct aaaccgtgct
      781 gaaggtgcgc tgttgatcaa tgcggtgggt gtcgatgatg tgaagcaagc ggatgagatg
      841 gtgaagctgg caacggcggt agcgcatctg attggtcgct caaatttcga tgcgatgagc
      901 ggtcagtatt acgcgcgatt cgtggtgaaa aatgtcgata actcagacag ctatctgcgt
      961 cagccgcacc gcgtaatgga gctgcacaac gacggcacct acgtcgaaga gatcaccgac
     1021 tatgtgctga tgatgaaaat cgacgagcag aacatgcagg gtggaaattc gttgctgctg
     1081 catctcgatg actgggaaca tctggaccac tatttccgcc acccgctggc gcgtcgcccg
     1141 atgcgctttg ccgcgcctcc gagcaaaaac gtcagcaaag atgttttcca tccagtgttc
     1201 gacgttgatc aacagggtcg cccggtgatg cgttatatcg accagttcgt ccagccaaaa
     1261 gacttcgaag aaggcgtgtg gctgagcgag ctttccgatg ccattgaaac cagcaaaggc
     1321 attctttctg tgcccgttcc cgttggcaaa ttcctgttga ttaacaatct attctggctg
     1381 cacggtcgcg accgctttac tccgcacccg gatctgcgcc gtgaactgat gcgtcagcgt
     1441 ggctatttcg cttacgcctc taaccactac cagacgcatc agtaagcgca aaggaattga
     1501 gcggatgtat gattttgtga ttattggcgg cggcatcatc ggcatgtcga ccgccatgca
     1561 actgattgat gtttatccgg atgcccgcat tgcgttgctg gaaaaagagt ccggtccggc
     1621 ctgtcatcag acaggccaca acagcggcgt gatccatgcc ggggtctatt acacgcccgg
     1681 cagcctgaag gcgcagtttt gcctggcggg aaaccgcgcc accaaagcct tttgcgatca
     1741 aaacggcatt cgctatgaca actgcggcaa gatgctggtc gccacctctg aacttgaaat
     1801 ggaacggatg cgcgcgttgt gggaacgcac ggcggcgaac ggtatcgagc gcgagtggtt
     1861 aaacgccgag gaactgcgcg agcgcgaacc gaatatcacc gggctcggcg gcatttttgt
     1921 gccgtccagc ggcattgtca gctatcgcga agtgacggcg gcgatggcaa aaatcttcca
     1981 ggccagaggc ggcgagatta tttataacgc cgaagtcagc gccctcagtg agcataaaaa
     2041 cggcgtggtg atacgtaccc gtcagggcgg tgaatatgaa gcatcgacgc tgattagctg
     2101 ttccgggctg atggctgacc ggctggtgaa aatgctcggc ctcgaaccgg gctttattat
     2161 ctgcccgttc cgtggcgagt atttccgcct tgcgccggag cataaccaga ttgttaacca
     2221 cctgatttac cccattcccg accccgcgat gccatttttg ggcgtgcatc tcacccgcat
     2281 gatcgatggc agcgtgaccg tcgggccaaa cgcggtgctg gcttttaaac gcgaaggcta
     2341 tcgcaagcgc gatttctcgt ttagtgacac gctggagatt ttaggctcgt cggggattcg
     2401 ccgggtgctg caaaaccatc tacgctcagg attgggcgag atgaaaaact cgctgtgcaa
     2461 aagcggctat ctgcggctgg tgcaaaagta ttgtccccgg ctttcgttaa gcgatctcca
     2521 gccctggcca gcaggtgtgc gggcgcaggc ggtatcgccg gacggcaagc tgattgacga
     2581 ttttctgttt gtcaccaccc cgcgcacgat ccacacctgc aatgcgccct ccccggcagc
     2641 gacatcagca attcctattg gtgcgcatat tgtcagcaag gtacaaacgc tgttggcaag
     2701 ccagagtaac cccggacgca cgctgcgagc ggcacgtagt gtggatgcct tacacgccgc
     2761 atttaatcaa taacctttga aaacaggacg taacaatgaa acttaacgac agtaacttat
     2821 tccgccagca ggcgttgatt aacggggaat ggctggacgc caacaatggc gaggtcatcg
     2881 acgtcaccaa tccggcgaac ggcgacaagc tgggtagcgt acccaaaatg ggcgctgatg
     2941 aaacccgcgc cgctatcgac gccgccaacc gtgctctgcc cgcctggcgt gcgctcaccg
     3001 ccaaagaacg cgccaccatt ctgcgcaact ggttcaattt gatgatggag catcaggacg
     3061 atttagcgcg cctgatgacc ctagaacagg gcaaaccgct ggctgaagcg aaaggcgaaa
     3121 ttagctacgc cgcctccttt attgagtggt ttgccgaaga aggcaaacgc atttatggcg
     3181 acaccattcc tggtcatcag gccgataaac gcctgattgt tatcaagcag ccgattggcg
     3241 tcaccgcggc tatcacgccg tggaacttcc cggcggcgat gattacccgc aaagccggtc
     3301 cggcgctggc ggcaggctgc accatggtgc tgaagcccgc cagtcagacg ccgttctctg
     3361 cgctggcgct ggcggagctg gcgattcgcg caggcgttcc ggctggcgta tttaacgtgg
     3421 tcaccggttc ggcgggcgcg gtcggtaacg aactgaccag caatccgctg gtgcgcaagc
     3481 tgtcgtttac cggctcgacc gaaattggcc gccagttaat ggaacagtgc gcgaaagaca
     3541 tcaagaaagt gtcgctggag ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg
     3601 acctcgacaa agccgtggaa ggcgcactgg cctcgaaatt ccgcaacgcc gggcaaacct
     3661 gcgtctgcgc caaccgttta tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat
     3721 tgcagcaggc ggtgagcaaa ctgcacatcg gcgacgggct ggagaaaggc gtcactattg
     3781 ggccgctgat tgatgaaaaa gcggtagcaa aagtggaaga gcatatcgcc gatgccctgg
     3841 agaaaggcgc gcgcgtggtt tgcggcggta aagcacatga acgcggcggc aacttcttcc
     3901 agccgaccat tctggtagac gttccggcca acgccaaagt gtcgaaagaa gagacgttcg
     3961 gccccctcgc cccgctgttc cgttttaaag atgaagccga tgtgattgca caagccaatg
     4021 acaccgagtt tggtcttgcc gcctatttct acgcccgtga tttaagccgc gtcttccgcg
     4081 tgggcgaggc gctggaatac ggcatcgtcg gcatcaatac cgggattatt tccaatgaag
     4141 tggccccgtt cggcggcatt aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca
     4201 tcgaagatta cttagaaatc aaatatatgt gcatcggtct ttaactggag aacgcgaatg
     4261 agcagcaata aagagttaat gcagcgccgt agtcaggcag ttcctcgtgg cgttgggcaa
     4321 attcacccga ttttcgctga ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt
     4381 gagtttcttg atttcgcggg cgggattgcg gtgctcaata ccgggcacct gcatccgaaa
     4441 gtggttgcgg cggtggaagc gcagttgaaa aagctgtcgc acacctgctt ccaggtgctg
     4501 gcttacgaac cgtatctgga gctgtgcgag attatgaatc agaaggtgcc gggagatttc
     4561 gccaaaaaaa cgctgctggt aacgaccggt tctgaagcgg tggagaacgc ggtgaaaatc
     4621 gcccgcgccg ccaccaaacg cagcgggacc atcgccttta gcggtgcata tcacgggcgc
     4681 acgcattaca cgctggcgct gaccggcaag gtgaatccgt actctgcggg catgggactg
     4741 atgcctgggc acgtttatcg cgcgctttat ccttgcccgc tgcacggcat cagtgaagat
     4801 gacgctatcg ccagcattca ccgaattttt aaaaatgacg ctgcaccgga agatatcgcc
     4861 gccatcgtga ttgagccggt tcagggcgaa ggcggtttct acgccgcgac gccagccttt
     4921 atgcagcgtt tacgcgcgct gtgtgacgag cacgggatca tgctgattgc cgatgaagtg
     4981 cagagcggcg cggggcgtac cggcacgctg tttgcgatgg agcagatggg cgttgcgccg
     5041 gatcttacca cctttgcgaa atcgatcgcg ggcggcttcc cgctggcggg cgtcaccggg
     5101 cgcgcggaag tgatggatgc cgtcgctcca ggcggtctgg gcggcaccta tgccggtaac
     5161 ccgattgcct gcgtggctgc gctggaagtg ttgaaggtgt ttgagcagga aaatctgctg
     5221 caaaaagcca acgatctggg gcagaagttg aaagacggat tgctggcgat agccgaaaaa
     5281 cacccggaga tcggcgacgt acgcgggctg ggagcgatga ttgccatcga gctgtttgaa
     5341 gatggcgatc acagcaaacc ggacgccaaa ctcaccgccg agatcgtggc gcgtgcccgc
     5401 gataaaggcc tgattcttct ctcctgcggc ccgtattaca acgtgctgcg catccttgta
     5461 ccgctcacca ttgaagacgc tcagatccgt cagggtctgg aaatcatcag ccagtgtttt
     5521 gctgaggcga agcagtagat ccgtacccat gccggaggcg acgctgcgcg tcttgtccgg
     5581 cctacgcgaa tccaggtcgg ataaagcgtt tacgctgcat ccgacaatct gtacgtgaac
     5641 aggaataaat ctatgtcggc cggataaggc ggagccgctc tccggtaaaa agaaccaata
     5701 acaattacac gcgtgacccg acgcgggaaa tgtcggggcg ctctcccaag tgacacactt
     5761 tcgagaggat tcaggatggg gcaatcatcg caaccacatg agttaggcgg cgggctgaag
     5821 tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt
     5881 tccagcgtcg ctatcgccga agcgggcccg gcggtattac tggcctatct gttcgccgga
     5941 ttactggtgg ttatgattat gcggatgttg gcggaaatgg cggttgccac gcccgacacg
     6001 ggttcgtttt ccacctatgc agataaagct attggtcgtt gggcgggcta taccatcggc
     6061 tggttgtact ggtggttttg ggtactggtt atcccgctgg aagccaacat cgccgccatg
     6121 atcctgcact cgtgggttcc aggcattccc atctggttat tttccctcgt cattaccctc
     6181 gccttaactg gcagtaattt attaagcgtt aaaaactacg gcgaatttga gttctggctg
     6241 gcgctgtgca aagtcatcgc tatcctggcc tttattttcc ttggtgcagt cgcaattagc
     6301 ggtttttacc cttatgccga agtgagcggg atctcaagat tgtgggatag cggcggcttt
     6361 atgcccaacg gcttcggcgc ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg
     6421 ggcgcagaaa ttgtcaccat tgccgccgcg gaatctgaca cgccggaaaa acatattgtc
     6481 cgcgccacca actcggttat ctggcgtatt tctatcttct atttgtgctc tatttttgtc
     6541 gtcgtggcat taataccgtg gaatatgccg gggctgaaag ccgttggatc ttatcgctcg
     6601 gttctggagt tgctcaatat tccccatgcg aaattaatca tggactgcgt gatattactt
     6661 tccgtaacca gttgcctgaa ctcggcgctg tataccgctt ccagaatgct ctactcctta
     6721 agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca accgcagtaa aaccccgtat
     6781 gtggcggtat tactctcgac cggtgcggca tttttaacgg tggtggtgaa ttattacgcg
     6841 cctgcgaaag tgtttaaatt tctgatcgac agctccggcg ctatcgccct gctggtttat
     6901 ttagtcatcg ccgtttcaca attgcggatg cgcaaaattc tgcgagcaga aggaagcgaa
     6961 attcgcctgc ggatgtggct ttatccgtgg ctcacctggc tggtcatcgg ctttattacc
     7021 tttgtgttgg tagtgatgct attccgcccg gcgcaacagt tagaagtgat ctccaccggc
     7081 ttattagcga tagggattat ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta
     7141 ttgtggcaaa aaacacccgt tcataatacg cgctgataat gatcaggagt cacaccatga
     7201 ccattacgtc tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc
     7261 aaccggatga aaaattacgg atgagtttgc ttacatcgcg ttatgcactt ggcgttgggc
     7321 cgttacggga agctctttcg caactggtgg cggaacggct ggtcacggtg gtgaatcaaa
     7381 aagggtatcg ggtggcgtct atgtcagagc aggagctgct cgatattttc gacgcccgcg
     7441 ccaatatgga agcgatgctg gtcagtctgg cgattgcccg cggtggcgat gaatgggagg
     7501 cagacgttct cgcaaaagcg catctgctca gtaagctgga ggcctgtgac gccagtgaga
     7561 aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca tacggcaatt gtggcgggct
     7621 gtggttctca ctatttgctg caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc
     7681 gatttatctg gctgcggcga acagtgcttt cggtggagat gctggaggat aaacacgatc
     7741 agcaccagac cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa
     7801 tgcgccagca tttactgacg ccaattccca ttatccagca ggcgatggct ggcaattaac
     7861 tactcttcag gaatacgcaa cacttgcccc gggtaaattt tatccgggct tttcagcatc
     7921 ggtttattcg cttcgaagat tttattgtac agattggcgt taccgtagac ctgtttagaa
     7981 atggcgctaa gagtgtcgcc agacttaacg gtataaaact ggctggcagc ggctggtgtc
     8041 gccgttttca cctgatcatc gacactggca ataccggaaa tattccccac cgcaacaagg
     8101 attttctcct tcgcctcctg acttaagccg tcacccgtga ccgtcgcttt gccgtcggcg
     8161 atttgaatgt tcactttatc ggcatcaggt ataccggttt tgttcagatg ctcctgcacc
     8221 ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag tttttctccg
     8281 gcatctttca caaaattgaa cagacccata gctacctcat tcgttaacgg aaacactcag
     8341 aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg gtgaacacag gtttaatcgc
     8401 gggtttgcac ccagaaagcg tgaatcaaac cggggatata cccaagcagt gtcagcagaa
     8461 tattaataat gaacgcccaa ccgaaccctt taccgagcag cacgccgagc ggcggcagaa
     8521 tgatggtgat gacgattctc cagaaaccca tatgtactcc ctataagaaa attactcatt
     8581 gtttaaaaag agattttatc tcttaagcgt agtaatttta gcggagcctg ccagtttcag
     8641 cttacgcttt tactctcatt tacgctgctg atgctggcat atgttatatt tttagtttag
     8701 agtttactta atttagaaaa tgcttaaata actatgactg aactcgcgca attacaggcc
     8761 agtgccgaac aggcagcggc cttattgaaa gcaatgagcc accctaaacg gttgctgatt
     8821 ctgtgcatgc ttagcggttc tcccggcacc agtgcgggag agctgacgcg cattaccgga
     8881 ctaagtgcct ctgcaacatc acagcatctc gctcgtatgc gggacgaagg gcttatcgac
     8941 agccaacggg atgcccaacg cattctatat tccattaaaa atgaggcggt aaatgccatt
     9001 atcgccaccc tgaaaaatgt ctattgtccg taaggagtca ctatggcttt gacaaccatt
     9061 tcgccgcatg atgcacaaga attaatcgca cgcggcgcaa agttaatcga tattcgtgac
     9121 gctgatgaat atcttcgtga gcatattcct gaagcagatc tggctccatt atccgtgctg
     9181 gaacagtcag gtcttccggc taaattacgt cacgagcaaa ttatattcca ctgccaggca
     9241 ggtaaacgca ccagtaataa cgccgataaa ttagcagcga ttgccgcccc cgcagaaatc
     9301 tttttactcg aagatgggat tgatggctgg aaaaaagcag gattaccagt agcggtaaat
     9361 aaatctcaac ccttgccgtt aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta
     9421 atcggcgtta tactcggtta taccgtaaat agcggtttct tcttattaag cggctttgtt
     9481 ggtgccgggt tactgtttgc aggaattagc ggtttttgtg ggatggcaag gttgttagat
     9541 aagatgccgt ggaaccaacg agcttgagaa gcgacgccgg acgcgccata gcggcggcat
     9601 ccggcctcag taattagatc aggaaatcgt cgagagattt accttctgcc agcgcctgag
     9661 cgattggctt cggtgtacgg ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg
     9721 tgaatttata tttcgccgga cgcggctggc gttttttacc agcgcgtggg gcggccgcgg
     9781 agctattacc caataactct tccgggttaa ttccgtcagc tttcatcagc tccagccagg
     9841 tgctaatttt ttcctggcgc tctgccagtt cacgctgctg ctgttcttct tcttcacgtc
     9901 tttctttagt gacaaccctg aatttttcga gcatttcttc aagaacgtca atggagaatt
     9961 cgcgagccat cgcacggagg gtgcgaatgt tatttaaact ttgtaacatt acggacataa
    10021 taaagaaaac cttttaacgc caaaa
//
LOCUS       DARNVM010000176         9997 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.176, whole genome shotgun sequence.
ACCESSION   DARNVM010000176 DARNVM010000000
VERSION     DARNVM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.176"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..267
                     /locus_tag="SI621_004025"
     CDS             <1..267
                     /locus_tag="SI621_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066680.1"
                     /translation="GDIWRRVTEGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRY
                     ALSYLAKREQKECGIILKCSGLPEKSNRGRPRLGSPLPETYSLV"
     gene            639..1022
                     /locus_tag="SI621_004026"
     CDS             639..1022
                     /locus_tag="SI621_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066681.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHSHTGHFSEEGVAE"
     gene            1019..1444
                     /locus_tag="SI621_004027"
     CDS             1019..1444
                     /locus_tag="SI621_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066682.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHVLSEQQKAACVVLNPEKSHPYIEDMEMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLYQDCGGALT"
     gene            1691..1888
                     /locus_tag="SI621_004028"
     CDS             1691..1888
                     /locus_tag="SI621_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HEI4066683.1"
                     /translation="MPQDDFCIVNPGGCIVYFPANGCDIQDIHDPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            2075..2482
                     /locus_tag="SI621_004029"
     CDS             2075..2482
                     /locus_tag="SI621_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HEI4066684.1"
                     /translation="MADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDT
                     TPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAA
                     NTYNAEPGVLEQTAQAYIESHGAIERHLGCSDF"
     gene            2597..2773
                     /locus_tag="SI621_004030"
     CDS             2597..2773
                     /locus_tag="SI621_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEI4066685.1"
                     /translation="MFFGSDYGSERTCCLNGIDPQAYRRHILSVLLKWLMAVKRDFTY
                     ICIKQDFTTSQHSL"
     gene            2828..3529
                     /locus_tag="SI621_004031"
     CDS             2828..3529
                     /locus_tag="SI621_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066686.1"
                     /translation="MFGHLLRYFSFCKKDLLVFHPESDYSDNLHKAFSFTKRLGFNEE
                     WNGVTKNKEYDYQDVFSHVCLQSGVTDFSASAGQCLKWSHYFQPYFENILECRVWVTV
                     GQLWKQERFIYNPSVADFQRWSEKGIQPEDFRHHSGFNFHAWLTTENGVIVDVSFMST
                     LSRRLPEHLSEVSGSVIIGTPETVLPEYKYVPMIVGQRIVEKIEKRSFIGFLAHDDID
                     LYTVPAILVPVWKEC"
     gene            complement(3723..3812)
                     /locus_tag="SI621_004032"
                     /pseudo
     CDS             complement(3723..3812)
                     /locus_tag="SI621_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            3893..4115
                     /locus_tag="SI621_004033"
                     /pseudo
     CDS             3893..4115
                     /locus_tag="SI621_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(4260..5879)
                     /locus_tag="SI621_004034"
     CDS             complement(4260..5879)
                     /locus_tag="SI621_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEI4066687.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKTAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAESLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGSPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDAPERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKGCSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESGNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFTRVHESGSAEFRRVAAGMEADAAAICEAISSHWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            complement(6265..6990)
                     /locus_tag="SI621_004035"
     CDS             complement(6265..6990)
                     /locus_tag="SI621_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4066688.1"
                     /translation="MHRNLPIYLQIREALRNGIAQNVYKQGERLPSENELAAQFNTTR
                     ATVVHAMQGLLTDGLIERIRGRGTFVKKPSIITVMDTQSLGFFEKDLVGVDKHLEYQL
                     VEFAPVSTSELLRTKLMLSGKESIHRLLRLRLINQQPIAFEIRYMPSILALQIDKQEL
                     EVHALQNIFEQALRIEIQTIANQVHVALPSRDAAQKLKIKRSRPILVRQHTYLTAENK
                     PVLWGETLYREEYEIHYSSHRQA"
     gene            7113..7910
                     /locus_tag="SI621_004036"
     CDS             7113..7910
                     /locus_tag="SI621_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEI4066689.1"
                     /translation="MIFDTHAHVFVRGLPLAEHCRYVPDYDATPESYLTHLDRFGIDV
                     GILVQPSFLGTDNHYMLEALRRYPTRFRGVAVVDPNITRCKLDEMAKLGVTGIRLNLV
                     GVEIPDLTQPAWQHLLEHIKALGWHVELHRAARDLPALITVLLKSGVKIVVDHFALPS
                     QEEKQNDPGFQFLLEHAETQQIWLKLSGAYRNGSTETLNDNVAPLIPLLLQHFGPAHL
                     LWGSDWPHTRYENVVNYQKTFELLTQQIPDKEMRNKILSVSAESLID"
     gene            7942..9291
                     /locus_tag="SI621_004037"
     CDS             7942..9291
                     /locus_tag="SI621_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066690.1"
                     /translation="MSIFYKKSWYKWIVLVVCILAYSSGNLVRWNYTGISQYLIGEWH
                     IGKEELGILGSAFFYAYAIGQSPWGTLTDILGGRRVISIGILITAIMFSLFAFAGGYH
                     AAIIIRVLMGFVGAATFVPCMAVLSRWFSKKERGMVLNIFSGTGGGLGELWSFLLMPL
                     ISLFMVGGATVLGLSSWRASTLIMAFVTLAIMILCFVGMRSDPSELGLPSVVEQETRK
                     QAKEHSYARTLLNALRDPWFWVITFAWQGFTVSLRLLPAWLPIYAAAYYKQTQGLSSV
                     EAMVAGGMIASVYVAGRILGTPVFGKLSDFLLVSYNVPRTLIIACFHIFITVSMFALS
                     HQMPSTFLFAVLVFVIGAAINMFPLVNAVVAEIWPVKTGGALMGMVNTVGQLIGASAL
                     AYSGFMAEKFSIAGAGYSFEYRGIWYLAMIFSSISILASLLAMHREKQSIRENALSH"
     gene            9297..>9997
                     /gene="citC"
                     /locus_tag="SI621_004038"
     CDS             9297..>9997
                     /gene="citC"
                     /locus_tag="SI621_004038"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEI4066691.1"
                     /translation="MFNHIFTQVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRD
                     EKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEA
                     LFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTQGH
                     RYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPHLTVHRGSEYIISRA
                     TFPCYFIKEQSVINH"
BASE COUNT         2485 a         2276 c         2448 g         2788 t
ORIGIN      
        1 ggagatatct ggaggcgggt taccgagggt gatggttact accactggtg ccggtttaat
       61 aaaaattatc cggtaaacat taaccacgtc attcactatt cagatcacaa agcggtgaat
      121 gccttacgct atgccctcag ctacctggcc aaacgagaac agaaagaatg tggcatcatc
      181 ctgaaatgca gcgggcttcc tgaaaaaagt aaccgaggtc gaccgcgact gggcagcccc
      241 ctgccggaga cttattctct ggtgtaagac cctgaacgac aactacgggc tggcgctaat
      301 tgttacagtc ggttccggag gccacttcag ggactctgcg cattttttac gacatggcta
      361 ttccgtatga accatacgga gatttaacca tggcacattc tttaccattc cttccggcaa
      421 ccgatacagc acatcctccg atgtccatcc ctgcctgctg acagtttttt acgccggagg
      481 gccattgtgt gacaaccaca gtgctgtaac agcaaccagc ctgctttacc caaactccag
      541 tccagatttt aactgaaccg ccattcacac cctgaccaca ctgcccgtac cgggacagct
      601 gttgcctgcg tgtcgtctgg ctgctgagga atatgacgat gtacgcaaaa tcctttattg
      661 catttgatgg caacgggcgt ctgacgggtg cccgtaccgc acagactgca ccttacgatc
      721 gctacacttg ccacctttgc ggcagtgcgc tcagatacca cccgcaatac gacactgagc
      781 gtccctggtt tgaacacact gacgacgggc tgacggagca cggtcagcag tgcccttatg
      841 tcagaccgga acgcagggag atacaactga ttaagcgtct gcagcaattc gtaccggatg
      901 ccttacccgt ggtgcgtaaa gccagccggc actgcagaca atgtcatcac gattattatg
      961 gggagcggta ctgcacgcac agccataccg ggcacttcag tgaggagggg gtggcagaat
     1021 gaacggtata aaaacggggc tggggatcac gccgggtgaa cacattatca gtgctgattc
     1081 tgcgctcagc agaaatatcc ggcagtgttt ctgcctttcc tgtcatggcc ggttgatttt
     1141 gcagacagat gctcaggggg cgtggtttga gcacgattta catgtcctga gcgagcaaca
     1201 gaaggcagcc tgcgtggtgc tgaatccgga aaagtcccac ccttacattg aggatatgga
     1261 gatgttttta tcgccattac cggtcgtgct ggagtggcat tgtgtgatgt gtgagcagtt
     1321 ttttcatggg aagaaattct gtgaggcctg tggtacgggg atttattgca gagcaacatg
     1381 tacgaagtct gtttacaggt accagccaga tttgtaccag gactgcggag gtgcactcac
     1441 ttaatccgaa atgcccgctc tgctcagggc gttcagagat atcgcaacct gcattttatc
     1501 cggtgtttcg cacctgcccc gttcgtgaca caaaggcgtg ataccggata tccgcagtcc
     1561 tgcttacctg aataaaaccc ggggtattga ttgtgtactg tggtgcgaaa tcgcaccgtg
     1621 atcgcggatt cgccgaatcc gcactgtggt gtcaaccaac catacgcgag agtcctctcc
     1681 gtatttatca gtgcctcagg acgatttctg catagttaat ccagggggct gcattgttta
     1741 tttccctgct aatggatgtg acatccagga tattcatgat cctgatttcc ctgagcagta
     1801 tcagcgctta ttggcacgtc tggattatct gacgaagccc gatgctaaag catcaggcca
     1861 gcgtaactct ggctacagcg gtttttgacc tgttgtaact gtaaggtgat ttattatgta
     1921 tacagaatca aacgttaagc tttcctgtaa cgccctctgg caaaagtgtg tccttgcaac
     1981 tctgtatcgg gctgcagcca cacatcctga aattaaatta ttacaattta aattaatacc
     2041 aggacagggt attaataagg ccattccctc tctgatggct gattttactg gtgaactgca
     2101 tagactcatt tctgaatatt tgcagtgtaa gcgacgtaaa gataaatctg ttgagcccac
     2161 ccctttatat tatctctgga gtagggaggc tgtggctaac gataccactc cttatcttat
     2221 ggtgctgcta cttaataacg atgtctggtg taagagtcgt gaggtggtgg ttctcagaaa
     2281 tctgattgag caggcgtggg gaaaagtatt gggtgaagat aactccatta cacatctggt
     2341 tgtgtatcct gaagtgctgt cgttggtatt tgatgcagct aacacataca acgctgagcc
     2401 tggtgtactg gaacagacgg cccaggctta cattgaatcg catggtgcca ttgaacgtca
     2461 tcttgggtgt agtgactttt aacgtcaggt gatgcataag ggcctttcgc tgagaggccc
     2521 tgtttacaca attaaataaa tcgagcccga ccaacaattt tgatgttaac agcaggatta
     2581 ttgaggggca atcaatgtgt tcttcggtag cgattacggt agtgaacgga cctgctgtct
     2641 gaatggcatc gatccgcaag cgtatcggcg ccatattctg agcgtactac tgaaatggct
     2701 aatggcagta aagagagact ttacgtatat ttgcataaag caggatttta cgactagtca
     2761 acattcttta taattcttca ggttcagagt atctgacata tagggttatg tattggggag
     2821 acatttaatg tttggccatc tgttacgata ctttagtttc tgtaaaaaag atttattagt
     2881 atttcatcct gaatctgatt actcagataa tttacataaa gccttctctt ttacaaaaag
     2941 gctaggattt aatgaagaat ggaatggggt cacaaaaaac aaagaatatg attatcagga
     3001 tgtattcagt catgtctgtc tgcagtctgg tgtgacagac ttttccgctt cagcaggaca
     3061 atgcttaaag tggagtcatt attttcagcc atattttgag aatatccttg aatgtcgggt
     3121 atgggttacc gttggacaac tctggaaaca ggaacgattt atttacaatc cgtcagttgc
     3181 ggattttcag cgctggtctg aaaaagggat tcagcctgaa gattttagac atcactcggg
     3241 gtttaacttt catgcgtggt tgacgacgga aaatggtgtg attgtggatg tttcctttat
     3301 gagtacgtta tcgcggcgat tacctgaaca cctgagtgaa gtcagtggaa gtgtgattat
     3361 tggcacgcca gagacggttc ttcctgagta taaatatgtt cccatgattg tcggtcagag
     3421 gattgttgag aaaatagaaa aacgctcttt cattggtttt ctggcgcacg acgatattga
     3481 tttatataca gtacctgcaa tcttggttcc ggtatggaaa gaatgttaaa gcacggaata
     3541 acactatgca cttaccgctt tccggaactc agcttgttac ctgagcggtc aacagagcag
     3601 tctttcagtg tctgcacttg gttgccacta aaccggagtc atttatcaga agtatatgtt
     3661 catggaagga tattgtcagc gccagtgata taagacggta atgaggatag ccaccccgca
     3721 tgggtttcct caacactggt cactgtgaca agcgcaccat gatcgtcttt gtatcgctga
     3781 ttgactttcg ataatagaac atcttctgac ataaatttta gtacggtaac aattaactaa
     3841 attttcatgt ggtagcactg aatcaggaga ctgcgtttcc tgatgtgtgc ttctgcagcc
     3901 gttaccggat acagacttcg ataccagtta cttcgacatc cgccatgtgt cctgggaccc
     3961 gctataacga agttggaggt aatcagtaca gcgttcccga aatgctgtgt ggtcagccag
     4021 tctcgatacg aatatcgctg aacgatgagt tgcggatcta cagtaatgag aaactggtgg
     4081 cctcacatcg cctctgctca gtgtcgtctg gctagcagat agtaccggaa cctcacgccc
     4141 cgctctggca gcagatcagt caggtggaac atcgaccact gagtgcctat gaggagctgt
     4201 tgtgatgcat gtgtcaacga cggatgaaag tggctcttcc gcaacctggg tggagatttt
     4261 catgccagtg gactcattac cctctgcctg agtagttcaa atcctgctcg accatacatc
     4321 tggcgtttca gcatcttcag gcgatttaca tgaccttcga caacaccatt actccagtga
     4381 ctactgattg cctcacatat agctgcagca tcagcttcca tccccgccgc cacgcgccga
     4441 aattctgctg aaccgctttc gtggactcga gtgaaccaac tgcttagctg tgatttattt
     4501 tgggttttca gaatacggta gaactcgagt gccagttgct gcgctatttt cagctcaggt
     4561 tctttttcac acatcagact aataaatcgg gaagcataat tttcttcatc cctggttatt
     4621 ctccagggca tcaaccagcg gcttacccgg gacactgaag gaagtctgac ggcagtagta
     4681 accggtgggt tccagccttt acgccatttg gcaaccgcat ccctgacgat ggtttcactg
     4741 cctgtaaatc cccgggccac catttcccgc catatccggc tggcgttata attaccactt
     4801 tctcgttgct cttttaacca ctccctccat gggtccagaa gccctcgctt tggcggtcgg
     4861 gttgacattt cagggaatgt tcctgaacgc acccagcgac tgactgtcac acgcgacagg
     4921 cctgtaatac gggatatttc cctgaaacta catcccttgt tatgcagggc cataacctct
     4981 gtccaacgct gatgccgttt tttgcgggtt tgttgtttta ggcgttccgg acgacggagc
     5041 gatgctgcag gcacagatgg ttctggatca ggtgatttct ttggtgacaa ctcactggca
     5101 acaagacgta tcagaggtat atgtctgtac atcattcgtt caggcgcatc gccaatattt
     5161 tttagcagat gccatcgatc ggccacctgt ctggcctgag gtgcaccttc acgtgctgct
     5221 gtcgcataga ctccactgcg atcacgcgag acaatctgta tttccggata ttttctgaac
     5281 caggtcgcca gcgtacgctg atcacggccg ggaagcagga cgaggggacg gtgagtatca
     5341 agattgacga ttaacgtacc gtaacggtgg ccccgatgcc acgcccactc atcaataccg
     5401 acatgaggcg agcctgactg tttcgtctcc ggggtattga taaccctgcg aaggagagta
     5461 tcagcactgc aacggagacc cactgccgtt gctgcccgtt tcccggcctc acctccggcg
     5521 attaatccca gttgacgttg taaattttgt aacgcctgtg aagactgttg gtgtgaaccg
     5581 gcgaagggag caagcgactc ggcaaaaatt ttacgtgaac aagccgggtt acggcagtac
     5641 cagtggcgga cagaaaatac aagccatagc gtatgtcctg agcagggtag atgctgtatc
     5701 cggcgccgac gacaactatg aacagagtcg ctacgcttaa agcattcagg acattgtgct
     5761 gttttgcgtt ttccgtggag atggagggtt ataccgtcag agctaataat ttgtcgactg
     5821 catcgccatc cgcaaggtaa ctgtagaagt gtcttaagag agtgcattga gttgcccatg
     5881 acttaaaaca gcttagatct ttgttcacca gaaagtttca tccattttct tccacccaag
     5941 ttgcggaaga acctgatttc aggcgttggg tgtggatcac tattgcaccg ttcgttacac
     6001 cagcgaaacc acacaggtag ctattaccat atgtggtaat acactagtta caggaaaaac
     6061 atgcaggttc cgcaatacgt tgtgtgttgc tgcttagtca ggatcttttc ttccatgcga
     6121 gatacgacaa cacttttgag gaaagatgtt cacaactggt ggggcgattg caacaggttt
     6181 gtgcagaact ccccccggat ggcaatgcag gggggaaagg atttttcagg ttgaccgccc
     6241 aggtacatca aatcagtatg ctgctcacgc ctgacggtgc gatgagtaat gtatttcata
     6301 ctcttcccgg tagagagttt ccccccaaag aaccggttta ttctcagcag tcaaataagt
     6361 atgttgtcgt accagaattg ggcgggaacg ctttattttc agcttctggg ccgcatcccg
     6421 gctgggcagc gccacatgaa cctggttggc aattgtctgt atctctatgc gaagtgcttg
     6481 ctcaaaaatg ttttgcaacg catggacttc cagttcctgc ttatctattt gcagggcaag
     6541 aattgaaggc atataacgaa tttcgaatgc aattggctgc tgatttatca agcgcaacct
     6601 taacagccga tggatgctct ctttgcctga taacatcagt tttgtccgta agagttctga
     6661 cgttgagaca ggtgcgaatt cgacgagttg atattcgagg tgtttgtcga caccaaccag
     6721 atctttttca aaaaatccaa gagattgcgt atccattacg gtgataatgg aaggtttttt
     6781 aacgaaggtt ccacgtcccc gaatgcgttc aatcaatcca tccgtaagaa gaccctgcat
     6841 cgcatggacc actgtcgctc gggtggtatt aaattgggct gcaagttcgt tttcagaagg
     6901 taagcgttcg ccttgtttat atacattttg tgctattcca ttgcgtaatg cttctctgat
     6961 ctgcaggtaa attggcaaat tgcgatgcat gtcaaattaa tccttcattc taagttgcat
     7021 agtcaactat acaacttcaa gggtgacggt atgtcatttt agcagatatc accttcaggt
     7081 gtggtcagtt aaacataaat gagttagaga aaatgatttt tgatacgcat gcgcatgttt
     7141 ttgttcgagg attaccatta gcggaacatt gccgatatgt tcctgattac gacgccacac
     7201 cggagagtta tctgactcac ttggatcggt ttggcattga tgttggtatt ctggtgcaac
     7261 ccagttttct ggggacggat aaccactata tgcttgaggc tcttcgccgt taccctaccc
     7321 ggtttcgtgg tgtagccgtt gtggatccaa acattacgcg ctgtaaactg gatgaaatgg
     7381 caaaattagg ggtcactggt atccgcttaa atttagtcgg cgtggaaatt cccgatctta
     7441 cgcaacctgc atggcaacac ctgttagaac atattaaagc cctgggatgg catgttgagc
     7501 tacacagagc tgcgcgcgac ttgccagcgt taattacggt attacttaaa tccggtgtaa
     7561 agattgtcgt tgaccatttc gcgttgcctt ctcaggaaga gaaacaaaac gatcccggat
     7621 ttcagtttct ccttgagcat gcagaaacgc agcaaatctg gttgaaactt tctggcgcct
     7681 atcgcaatgg cagtactgaa actctcaacg ataacgtggc accgttaatt cctctcctgc
     7741 tgcagcattt tggacctgca catttattgt ggggtagcga ctggccacat actcgatacg
     7801 aaaatgtcgt caattatcaa aaaacgttcg agttactgac gcagcaaata cctgataaag
     7861 aaatgagaaa taaaatttta tcggttagtg cagaatcact tatcgattag ttggattgag
     7921 aatatagtcg aaaggtatat catgagcata ttttataaaa aaagctggta caagtggatt
     7981 gtattggtgg tttgtattct tgcctattca tcaggaaacc tggtgcgctg gaattatacg
     8041 ggaatatcgc aatatcttat tggtgaatgg cacattggaa aagaagaatt ggggatcctt
     8101 ggttccgcat tcttttatgc ctacgcgatt gggcaaagtc cctggggaac acttaccgat
     8161 atattaggcg gacgcagagt tatttctatc ggtattttaa ttaccgctat tatgttctct
     8221 ctctttgctt ttgcgggtgg ctaccatgcc gctatcatca ttcgagtatt aatgggattt
     8281 gttggggcgg caacatttgt gccttgtatg gcggtacttt ctcgctggtt tagtaaaaag
     8341 gagcgaggta tggtgctgaa tatcttctct ggcacaggtg gtggattagg tgagctgtgg
     8401 tcctttttat taatgccatt gatttcatta tttatggtag gcggcgctac cgtgcttggc
     8461 ctgagctcct ggcgtgcatc tacgctaatt atggcgtttg tcacactggc gatcatgatc
     8521 ctctgttttg tcggtatgcg ctccgacccg tcggaattag gtcttccgtc cgttgttgaa
     8581 caggagacgc gtaagcaggc gaaagaacat agctatgcca ggacactgct taatgcgttg
     8641 cgcgatccgt ggttctgggt cattacattc gcctggcaag gttttaccgt ttctctgcgt
     8701 ctgctaccgg cctggttgcc tatctatgcg gcagcttact acaaacagac acagggatta
     8761 tccagcgttg aggcgatggt cgcaggaggt atgatagcat cagtgtatgt tgccggacgt
     8821 attttgggaa cgcctgtctt tggcaaatta tctgactttt tgcttgtcag ttataacgta
     8881 ccaagaacat tgattattgc ttgcttccat atctttatta ccgtctcaat gtttgctctt
     8941 agccatcaaa tgccttccac atttttattt gcagttctgg tttttgtgat tggtgccgcg
     9001 ataaatatgt tccctctggt taatgcagtc gtcgcagaaa tctggccggt taaaacgggg
     9061 ggcgcgttaa tggggatggt gaatacggtt gggcagttaa taggtgcctc agcgttggcc
     9121 tacagtggtt ttatggcaga aaaattttcc atagccggag caggatacag ttttgagtat
     9181 cgtggcattt ggtatttggc aatgatattc agttcaatat ctatcctggc gtctctgttg
     9241 gcgatgcatc gcgaaaaaca aagtattcgg gaaaatgcct tgagtcatta agcataatgt
     9301 ttaatcatat atttacccag gtcaaacgtt cagaaaataa aaaaatggcg gaaatcgccc
     9361 aattcctgca tgaaaatgat ttgagcgttg acaccactgt cgaagtattt attaccgtaa
     9421 cccgcgatga aaagcttatc gcgtgcggcg gaattgccgg aaatattatt aaatgcgttg
     9481 ctatcagtga atccgttcgc ggtgaaggtc tggcgctgac attagccacc gaactgataa
     9541 acctcgccta tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg
     9601 cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt ccccggcgtg atggtgctga
     9661 tggaaaacag cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc
     9721 cagggaacaa gattggctgc attgtgatga acgccaatcc ctttacacag ggtcaccgtt
     9781 atctgattca acaggctgcg gcacagtgcg actggctgca tctgttttta gttaaagaag
     9841 attcttcacg cttcccctat gaagaccggc tggatctggt attaaaaggc accgccgata
     9901 ttccacatct gactgtgcat cgcggctccg aatacatcat ctcccgtgcc acgttcccct
     9961 gctacttcat taaagaacag agcgtcatca accattg
//
LOCUS       DARNVM010000177         9964 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.177, whole genome shotgun sequence.
ACCESSION   DARNVM010000177 DARNVM010000000
VERSION     DARNVM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.177"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..577
                     /locus_tag="SI621_004039"
     CDS             <1..577
                     /locus_tag="SI621_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HEI4066692.1"
                     /translation="QKQIKESHKESATNVTFDYKELAPIFEGVQKKQIEALSNQLEHI
                     KSFKTDYDSRLNKFARDFQYLSSAFSINRQDLLKNYQEIRASITDYDDLLREIKELTI
                     SRDKSVDDKRTLFDKKRDNWNSQEVQDEVKALNEDIADSDNKIRSKLTIVQNHRRENQ
                     YKANKNISNAMLNILDWFERYPDIVKNITQA"
     gene            complement(714..1379)
                     /gene="yieH"
                     /locus_tag="SI621_004040"
     CDS             complement(714..1379)
                     /gene="yieH"
                     /locus_tag="SI621_004040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HEI4066693.1"
                     /translation="MTQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDQEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPELMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELGQLPALWKARSWN
                     ITR"
     gene            1546..2883
                     /gene="adeP"
                     /locus_tag="SI621_004041"
     CDS             1546..2883
                     /gene="adeP"
                     /locus_tag="SI621_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HEI4066694.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTECVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2937..3503)
                     /gene="yieF"
                     /locus_tag="SI621_004042"
                     /gene_synonym="chrR"
     CDS             complement(2937..3503)
                     /gene="yieF"
                     /locus_tag="SI621_004042"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HEI4066695.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKS"
     gene            complement(3525..4274)
                     /locus_tag="SI621_004043"
     CDS             complement(3525..4274)
                     /locus_tag="SI621_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEI4066696.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(4431..5390)
                     /gene="yidZ"
                     /locus_tag="SI621_004044"
     CDS             complement(4431..5390)
                     /gene="yidZ"
                     /locus_tag="SI621_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HEI4066697.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKHLYASMA"
     gene            complement(5365..6540)
                     /gene="mdtL"
                     /locus_tag="SI621_004045"
     CDS             complement(5365..6540)
                     /gene="mdtL"
                     /locus_tag="SI621_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HEI4066698.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTNSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6673..7920)
                     /gene="tnaB"
                     /locus_tag="SI621_004046"
     CDS             complement(6673..7920)
                     /gene="tnaB"
                     /locus_tag="SI621_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HEI4066699.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(8012..9427)
                     /gene="tnaA"
                     /locus_tag="SI621_004047"
     CDS             complement(8012..9427)
                     /gene="tnaA"
                     /locus_tag="SI621_004047"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEI4066700.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9648..9722)
                     /gene="tnaC"
                     /locus_tag="SI621_004048"
     CDS             complement(9648..9722)
                     /gene="tnaC"
                     /locus_tag="SI621_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HEI4066701.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT         2628 a         2411 c         2447 g         2478 t
ORIGIN      
        1 ccaaaagcaa ataaaggagt ctcataaaga atctgctaca aatgtaacat ttgattataa
       61 ggaacttgca cccatttttg aaggtgttca aaaaaaacaa attgaagcgc tatcaaacca
      121 actggaacat ataaaatctt ttaagacaga ttacgattca aggctcaata agtttgctcg
      181 cgattttcaa tatttatcca gtgcattttc aattaacagg caggatttat taaaaaacta
      241 tcaggaaata agagcttcca ttacagatta cgatgactta ttgcgagaaa ttaaagagtt
      301 gaccataagt cgggataaat cagtagatga taaaaggact ttatttgata aaaaaaggga
      361 caactggaat agtcaagaag tacaggatga agtaaaggca ttaaatgagg atattgctga
      421 ctctgacaat aagatacgtt ctaagcttac tattgttcaa aatcatagac gcgagaatca
      481 atataaagcg aataaaaata tcagtaacgc gatgcttaat attctggatt ggttcgaacg
      541 gtatcccgat attgtaaaaa atataaccca ggcttaacta tgctgagggt ggatgacagg
      601 acgaataacg acataaatta ttcataggga atagacgctt actcatcaga cacctggtat
      661 tctttatctc aaaccagcgt gactgatgtg taagcgttga agtaaggagg cgattatcgc
      721 gttatattcc agctgcgtgc cttccagagc gcaggcagct gccccagctc ggtaaaggtg
      781 gtgactttcg gatgaacgat cggcttattg tgcgggtcag cgcagaagta aaacacttcc
      841 ataccggcat cgattcctga ttgcgcaccg gcgctggagt catcgaccag aatgcagttt
      901 tcaacgttca cattcatcgc ttttgccgca tggaacatta actccgggtc gggcttccaa
      961 cgctgaatat cgtagccgct gaacagtttt tccgggaagt agtgcagcat attgagctta
     1021 ccaagcgaat gctgcatttt gctgaccgga ccgttagaga cgacgcacat cggcacgtta
     1081 atgctcgcca gcaattcgtt cgctcctgcg atgtccgcaa gttcggtatc aaacagacgt
     1141 gctacttctg cgcggtaaac gtgctcggca tcggcctgga ccatcgtcac gccctgttct
     1201 ttactgatga tgtcgatgat ttcgtagagt tttacgcctt taaaacgggt aaaaatctct
     1261 tcctggtcaa cgtgaatgcc aaattccctg aacatcgtga cataggcgcg agagcaaatg
     1321 acctcgctat cgaccagcgt accgtcgcag tcgaaaaata ccgcttctat ctgggtcatg
     1381 tctttccttt atcgttagtt taacgttttc gtcatgccaa ttcgcatcac gcaatcgttg
     1441 ccgtataagc aaatacaatg aaaaaaagtt atcttcagcc gtccatattg tctcattttg
     1501 gtatacgata gcgacggatt ttccctcctt gttcggaaat agataatgag tcaacaacac
     1561 acaacccagg cttctggcca ggggatgctg gaacgcgtgt ttaaactgcg cgaacatggc
     1621 acgacggcac ggaccgaagt gatcgccggt tttaccacct tcctgacgat ggtttacatc
     1681 gtttttgtta acccgcaaat tcttggcgtt gctggcatgg ataccagcgc cgtcttcgtt
     1741 actacctgtc tgattgctgc attcggcagt attatgatgg gactgtttgc taacctgcca
     1801 gttgcgctgg cacctgctat gggcctgaat gcgttcttcg cttttgtggt tgtacaggcg
     1861 atgggcttgc cgtggcaagt cgggatgggc gcaatcttct ggggcgcgat aggtctgctg
     1921 ttactgacga ttttccgcgt tcgctactgg atgatagcca acattccggt gagtctgcgt
     1981 gtgggcatta ccagcggtat cggcctgttt attggcatga tgggactgaa gaatgcaggt
     2041 gtgattgtcg ctaacccgga aacgctggtg agcatcggta atctgacttc tcacagcgta
     2101 ctgctgggga tcctcggctt cttcatcatt gctattctgg cctcacgcaa cattcacgcg
     2161 gcggtgctgg tttccatcgt ggtgacgacg ctgctgggct ggatgctggg tgatgtccac
     2221 tacaatggca tcgtttctgc gccgccgagc gtaatgactg tcgtgggtca tgtagattta
     2281 gccgggtcgt ttaaccttgg gctggcaggg gtgattttct ctttcatgct ggtcaacttg
     2341 tttgactcct ccggtacgct gattggcgtg acagataaag caggactggc ggatgaaaaa
     2401 ggtaaattcc cgcgcatgaa gcaggcactg tatgtcgaca gcatctcttc cgtgaccggt
     2461 tcgtttatcg ggacttcttc tgttacggcg tatatcgagt cttcttccgg cgtttccgtt
     2521 ggggggcgta ccggcctgac ggcagtcgtt gttggtctgc tgttcctgct ggttatcttc
     2581 ctgtcgccgc tggcggggat ggtgccggga tatgccgcag ctggcgcgct gatctacgtg
     2641 ggcgtactga tgacctcaag tcttgctcgc gtgaactggc aggatcttac tgaatgtgtt
     2701 ccggcgttta ttaccgctgt gatgatgccg ttcagcttct cgattaccga aggtattgcg
     2761 ctgggcttta tctcctactg cgtgatgaag attggtaccg gacgtctgcg tgatctaagc
     2821 ccgtgcgtaa tcatcgttgc gctgctgttt atcctgaaga ttgtgtttat cgacgctcac
     2881 taaaatcgat agcgcgttaa aaacccgcca tgcatgtttg ctggcgggtt ttttatttag
     2941 ctcttaactc gctgaataaa ctcaccaaat gcagtcaatt gcccggtcag gtggtccagc
     3001 gtaccctgat caatcacttc tccggtttgc ggatcaactt tgttctgaat cacgccgccc
     3061 ataaattccg gcttgttcat caccattgca tcgaggaaaa ccagaatctg gcgcaggtga
     3121 tactgacagc gcgcgccgcc aatcacgccc attgagctgg tctgaatcaa caccggttta
     3181 ccggccagag gttgatccgg caggcgggaa agccagtcga tggcattttt cagcccaccc
     3241 ggtaccgagt agttatattc cggcgtgacg atcaccacac cgtcagcctg acggatctgt
     3301 tccgccagag cttcaaccgt tgctggaaaa ccttcttcct gctgtacgtc agcgtcatac
     3361 aagggaatgt cggcaatgga aggtaacgca ttgacttcca tgctcgccgg agcaattttc
     3421 ggcagggtac gtgcaaccat gccattaaat gagcctttgc gcaggctccc cagtaacgta
     3481 accacctgca atttttcaga catgatgact cctgtggttg tctatcaatt tggcgaaaaa
     3541 gccttcacgg tagagagctg ggcaaaacgc atcatccggc tggtaggatt gttggcgcgg
     3601 ctgtgaatat cgggcaggct tttccagccg tgtttgccat ctaactccca gacactgagc
     3661 ttttcaatcg tgggcgtgac cgccacggac cacaccagca cgtcctccgc gtcgcataac
     3721 gcttctacat gatttacatg agcacatttc aggcgtccgg caatgggcag cagctgcaga
     3781 tcgcgcggat cgtcattcaa aacatcaccg gttagtttta gcacgcttcg gcgcagcgtg
     3841 atgagctgcg cccgcgcttc gtttggatca ttttgtttac tgatccatag cgattcattg
     3901 ctggaaaagg tgtggttatc gggcgcgtgt ggactatgaa acccgcgcgt cgcacgctgc
     3961 agttccatat cgaggccaca ttcaccttcg gttgttaacg ccacgccaac catattcccg
     4021 gcataggaaa tagaaaacga aggcaaattt ttatcgctga aaaccggttt acctttcggc
     4081 agggtgacga tttccggcaa ttcgccaatg ccatacagca tgaacatcag ttctgcgagt
     4141 aaacctctgg acgccagaaa acggctttga cgatgagggg gaatgtttcg ggcttcttga
     4201 tgacactggg aggggagacg aacagaaatc agatgtcctt ccgttaaaat ccctcgggca
     4261 aagtgagtcg ccatttttcg ctccatgctt aatggtccat tatgaataca agagtaatca
     4321 tttactggaa tgatggacag actaaagcgg ctaaaagttc gattcagaca agcttattac
     4381 aactttttta cattttattg tgaaattggg tcgcgtttca tacgattcgg ttatgccatc
     4441 gacgcgtaaa gatgtttaat ggtttcccgt aaccagacga tcttcggatt atggctgttc
     4501 cgtttatgcc acagcagggt aaaagggact tccagctttt tttgctggct ttcgtcaaac
     4561 gggagaggaa gagcaaccaa cggcagttgg tggagttgat tgtagtactg acagtagcgc
     4621 ggtgcggtcg ccagtagcag attgtcgggc tgtgctgcca taaacagtga ctgctcgaat
     4681 tccggcaggc tcatagcaat cgtgcgttcg cgccccagct cctgtaacac attgtccagc
     4741 gcccaggtat cgctctgttc ccagcaaatg ctgatatgcg gataacgtaa aaaggtgtcc
     4801 agattccacg tttcatgcag tgccggatga tcttttcgta accagacgca gggcacatca
     4861 ctaaacagca cttcataatc aatggctaac ggtagcgagc ttaacagctc ccgcgaacgg
     4921 ggatgacttt cgcgaccgga aaaaccaata tccacttcac cacgagtaat ggcatctaag
     4981 gaatcgtaat cccagttacg taatttgatg gtcgcctgcg ggtaacgttg gtagatccgt
     5041 ttcgacagcg cattaagcat gatcatcatc agcggcgatt ccgccgccag ctcaaacttc
     5101 aggccgcgcg gtgtctggtg atgcggttta tccagcagca ggttgctcat ttgcatccac
     5161 tccgccagat tttgctccat gctgaccatt agcggtgtgg gcgacagccc cagcggtgag
     5221 ttcacaaaga gcgggtcgtc aaaccacgct cttaactttg ccagcgactt actcaccgcc
     5281 gaaggggtca cgttcatccg cttcgccgct ttggtcacgc tgcgctcctg catcagcagt
     5341 tgcagacaga gcagcagatt gagatcaagc gtggtgatgg atttcttcat gagcggcaac
     5401 cgggcgtcca ggcgcgacga acataatcag caacaggctc actatgctac aggcaatcag
     5461 aatcccgatc agcatattcc atgcgccgat accaaccacc gctgccagcc aaatccacag
     5521 tgacgaaccg caaacctgcg caatacctaa ggtcgagctg gctacgcccg cgcgtaatga
     5581 aaacggccct aacgcctgac tcatcgctac gccaaaacct accgagaaac cggcgcaaat
     5641 cagcgtgata ccaaacagag aaaccgcatg ggaaggtgaa acggcaaggg tgatgcccgc
     5701 cgccaggaat aatacctgcg aggtgatcat caacgtacgt ggcttaaaaa ttcccagcgc
     5761 aaatggcgtg gagaatgaaa cggtcatgct gacgccagcg gtcaacgcca taatggtggc
     5821 gtattctccg cgctcaaaac ccatgatttc catcagcaat accggcgacg tgttgacgaa
     5881 ggtgaggatc accgaaacgc tgagggtggt gataacaaca cggctgagga aaaaccggtt
     5941 aagcagcgac tcgctatttt ctcgtggttt gtccgaattt gtgggggccg cagggcgcgt
     6001 ttcttttaaa ataaacaaag acaacatcag taccgcgatg cccatcgttg ccatcgtcca
     6061 gaacagactc tgccacggga atttaagcat aatcaggtgt ccgagcactg gcgctaacac
     6121 cggaatgatg caggtaatgc cgttgagtaa cgacagcact ttagcccgac gtcgatcatc
     6181 cagtgtgtcg cgtaaaatgg cgaacgcaac cacgtaacaa cagcccgcgc ccagcccctg
     6241 tagaaagcgc cctgcaagaa ataacgtgct ggtttcagcc agtgaacaga acaccgaggc
     6301 gataataaat agcgccgcgc cgggtatggc gacaggcttt ctccctgaac gatcggccac
     6361 tttaccggca aataacatcg cagctgccat gcccgccaga taaacggaga acgcaatatg
     6421 caactgcgct tcgctggcat tgagatcggc ggcgatgcgc ggtaaaccaa cgaggtacat
     6481 atcaatcccg gcgggataaa gtaaaaccag ggcaaaacta caaatcaaaa agcgggacat
     6541 gggctaaagc gtcctgatag taaggtgacg caaagcatac gtggtgaagg agaatcaggc
     6601 gagttgccat ttggacataa gtgatttcct gacaggcaat aagccagcga aatggctggc
     6661 ttcttgaagg gatcagccaa atttaggtaa cacgttaaag acgttaccga accagcataa
     6721 aattacggtt ataccaaaga gaatgacaat cgccggaata agattgccgc cccagaccgt
     6781 gaacatctga ttgggaaact tcttgcgagc tttgattgcc agcaccgcgg ggataatgac
     6841 cgcccagatg gtggcacaca gtccggcacc gccgatcccg taaatgaagc cgttcgggaa
     6901 gatcagatac aacaacgcag gtggcaggaa ggttaacagc acggttttga aacgcccgcc
     6961 gtgggagtta tcaatcttaa acagatccgc cagataatcg aacaacccca gcgtgacacc
     7021 aaagaacgaa ctggcaacag ctaagttgga gaacaccagc aggcaaaact cgataatgcc
     7081 gtgctgtttg gtgccgagga acgatttgac cagcgaatca acgttgccgc ctgaggagat
     7141 tatcgcctta aagctttctc gtgggatatt ccccatcgtg caatagagcc agaagagata
     7201 aatcaccagc gccagcagcg aaccgaagac cacgcttttg attagcttat ctttgcgttt
     7261 gccatagcaa ataatcaggc tgggaatatt gccgtggaaa ccaaatgacg ccagacacac
     7321 cggcaaagcc ataaagatat acgggaaata agatgtaccc gcagtggtgc tggtggaatc
     7381 gcgcagaatg gagtaatcga cctggaagaa gaaagaacca aaaacaatta caaaggagat
     7441 aatcttcagc ccaaggaaca atgaggtaat acgactggcg gctaacgagc ttatccacaa
     7501 tacgctggca acgaaaatgg ctgtgcagat cccgacaata cgtggattag cgtgataacc
     7561 caaattcatt gatatcgttt cactaatgat cgcaccatta gcagagatat aggcataagt
     7621 gaggatatag agaacgaagg caacggtaat accgctgata atgttccagg tgttaccgat
     7681 taaatctttg gtgatggtgt taaaactgga gccgacggga taatttaaat ttgcttctaa
     7741 taacaacaac ccggaatgaa gcattgaaaa ccaggcaatg ataaggataa aggcacccca
     7801 gaaaaaccag gcaccggcaa gatcaacagg taaagcaaac atacctccac caattactgt
     7861 acctgctata accataacac cccaaaatgc agagtgcttt ttttcagctt gatcagtcat
     7921 gatgccacct ttagaggaag gctatttttg ttattgagga tgtagggtaa gagagtggct
     7981 aacatcctta tagccactct gaatgtttta attaaacttc tttcagtttt gcggtgaagt
     8041 gacgcaatac tttcggttcg taggtaaagg ttaacccttt aatattcgcc gcgttctctt
     8101 tcacatgttt aaaggcttca ataatgaagt ccatatgtgt ttgagtatat gttgcacgcg
     8161 gaatggttaa acgcagcagt tcagccgggc atggcagttg tttaccggtt ttcggatcgc
     8221 ggcctaacag gaaagaacca atttctaccg cacggatacc ggcgacttta tacagctcgc
     8281 acgccagcgc ctgtgccggg aactggtctg ccgggatatg cggcagcagt ttaccggcat
     8341 caacgaatgc cgcgtgaccg cccgcctgct ggcagacaac gccaatctct tccagaccat
     8401 cgaccagata ctgtacctgc gcgatacgat aagccagcca gtccagattc atgccgtcat
     8461 acagacctac cgccagacgc tccatcgcgc cgccttccag gccgccatat gtcgggaagc
     8521 cttcctgtac cacgcaaagg gttctgcatt cggtgtacac atcaaagaag ctgtcgtctt
     8581 tcatgcacag caagccgccc atcggcacca tcgcatcttt cttggcggac atcgccagca
     8641 tatcggcata tttgtaggtt tcgcgggtga tctgctcgat ggtccagtct ttgtattctg
     8701 cttcacgctg cttgatgaaa taggcgtttt cagcaaagcg tgcggagtcc attaccaccg
     8761 gaatatcgta tttcttcgcg atgctgtaca tcgcttttaa gtttgccagt gaaaccggct
     8821 gaccacctgc agagttactg gtgatggtcg caacgatata cggcacgtta ttcgggccaa
     8881 cttcttcaat gccgcgttct aatccctcaa ggtcaaagtt gcctttaaag tcgtaacgca
     8941 cgcccgtatc gaaggcttct ttgatataga cgttacgcac ggtacagccg ttgatctggc
     9001 tatggccctg cgtggtatca aagaaatagt tagagaacgc caccattttg ctgcgatcca
     9061 ggcctttttc ctgctcgcgt tttttaatca gtaccggaat atagatttgt tctgcaccac
     9121 ggccctggtg agtcggaata gtgtattgat aaccaaagat attttttact gactcggcta
     9181 acgcatagta gctgcggctg ccgctgtaag cttcgtcgcc gcgcatcatc gcggcctgca
     9241 tactctgcgt caccgccccg gtgccgctgt cggtcagtaa atcgataaac acatcttcgc
     9301 tatccagcag gaacgggttc ataccggatt taataattgc ttcttcacga taagcgcgag
     9361 tggtacgttt tactggctca ataacacgga tgcggaacgg ttcagggaga tgtttaaagt
     9421 tttccattac ataatccttc atttatttta attacagtga tccctgtgaa tattacatct
     9481 gcaatatcca ttaattcgcc agatatatat gcaagcacca tcatttacta aaacaagtaa
     9541 ataaacgaga gatggccttt gcaaaaggca aaattaagat atagcaaaca aatagatcac
     9601 attgaatcta atcggtttgg ctctggtgat ggctacagaa gggcaaatca agggcgatga
     9661 tcaacaatct tgttgtcaat attgaaccat tttgaggtca cacatatatg taagatattc
     9721 ataatgcact tatcctcgaa agacacgagg ctacagtata atactcaaga aatttgtcta
     9781 ttctgaaatt gtttaaatgt gaatcgaatc acaattagat attggatgtg tttgtgatag
     9841 ctgccccatg caaaatatta gcaaaaacac atgaatggat aaatttttta atttttatca
     9901 ttattgttga aggtgatggc attgattata ggggttccat tacaaggagc ggtttccatc
     9961 gttg
//
LOCUS       DARNVM010000178         9960 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.178, whole genome shotgun sequence.
ACCESSION   DARNVM010000178 DARNVM010000000
VERSION     DARNVM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.178"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..180)
                     /locus_tag="SI621_004049"
     CDS             complement(<1..180)
                     /locus_tag="SI621_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066702.1"
                     /translation="MLQSSADKACNVLVKKARYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYKFTKRIYFI"
     gene            258..1352
                     /gene="ddlA"
                     /locus_tag="SI621_004050"
     CDS             258..1352
                     /gene="ddlA"
                     /locus_tag="SI621_004050"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEI4066703.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1376..1588)
                     /locus_tag="SI621_004051"
     CDS             complement(1376..1588)
                     /locus_tag="SI621_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HEI4066704.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1848..2156
                     /locus_tag="SI621_004052"
     CDS             1848..2156
                     /locus_tag="SI621_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HEI4066705.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHVPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2214..3308)
                     /gene="yaiW"
                     /locus_tag="SI621_004053"
     CDS             complement(2214..3308)
                     /gene="yaiW"
                     /locus_tag="SI621_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HEI4066706.1"
                     /translation="MSRVNHLSSLSLLAVLVLVGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3321..4541)
                     /gene="sbmA"
                     /locus_tag="SI621_004054"
     CDS             complement(3321..4541)
                     /gene="sbmA"
                     /locus_tag="SI621_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HEI4066707.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4893..6050
                     /gene="ampH"
                     /locus_tag="SI621_004055"
     CDS             4893..6050
                     /gene="ampH"
                     /locus_tag="SI621_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HEI4066708.1"
                     /translation="MKRCLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNIRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6051..6674)
                     /gene="iprA"
                     /locus_tag="SI621_004056"
     CDS             complement(6051..6674)
                     /gene="iprA"
                     /locus_tag="SI621_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HEI4066709.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6760..9708)
                     /gene="ehaB"
                     /locus_tag="SI621_004057"
     CDS             complement(6760..9708)
                     /gene="ehaB"
                     /locus_tag="SI621_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HEI4066710.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGKGWNADDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILNVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRTGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSINNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2583 a         2453 c         2367 g         2557 t
ORIGIN      
        1 tatgaaatat attctcttcg taaacttata attaagagaa aaaaactcac tgcatggcca
       61 tacaagccag gaataaatta gcttcccgtt catcattttt gtgatcttgc gcacatcatt
      121 gagataacgc gctttcttca ccagtacgtt gcacgctttg tcagcagaag attgcaacat
      181 tctgtcagaa ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga
      241 cgggataggt ttttaagatg gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag
      301 cggaacatga agtgtctctg caatcggcaa aaaacattgt cgatgccatt gataaaagtc
      361 gcttcgacgt tgtgctgctg ggcattgata aacaagggca atggcacgtc agcgatgcca
      421 gcaattatct gctaaatgca gacgatcctg cccatattgc gttgcgccct tcggcgacca
      481 gccttgcgca ggtgcctggt aaacatgaac atcagcttat cgacgcgcaa aacggtcagc
      541 cgttgccgac ggtggatgtc attttcccga ttgtccacgg tacgctgggc gaagatggtt
      601 ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt aggttctgat gttctggctt
      661 cagcggcctg tatggataaa gatgtcacca aacgtctgct gcgcgatgcc gggctgaaca
      721 ttgcgccatt tattaccctg acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg
      781 agtctaaact ggggttaccg ctgtttgtaa aaccggctaa tcagggctct tctgttggtg
      841 tcagcaaagt aaccagtgaa gaacagtacg caattgccgt cgatctggcg ttcgagttcg
      901 accataaagt gatcgttgag caagggatca aaggtcgtga gatcgaatgc gcagttctgg
      961 gcaacgacaa tccgcaagcc agcacctgtg gcgagatcgt actcaccagc gatttttatg
     1021 cctacgacac caagtacatt gacgaagatg gcgcgaaagt ggtagttccg gcagccattg
     1081 cgccagaaat caacgataag atccgggcga ttgccgttca ggcttatcag acgttgggat
     1141 gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg
     1201 agatcaacac actgcctggc ttcaccaaca tcagtatgta tccgaagctg tggcaagcca
     1261 gcggtctggg ttacaccgac ctgatcaccc gtttgattga gctggcgctg gagcgtcacg
     1321 ctgcggataa cgcattgaaa accacaatgt aatatttaaa cgcccgataa tattcttatt
     1381 gggcgttttc agtttcttca tcgcgacgac gaataatcag ccccgccagc cagaaactaa
     1441 tcacccacgt caccagaccg acggcatagg tctgccaacc ttttgcttca aatcccagta
     1501 accccaccac gccattaaga atgaatataa ggccaatggc gaacgcatag tagtgccagt
     1561 cacggcggat ttttacaggc aagttcatgg cagctccgtt aacgaaaaac aacatccttg
     1621 catattctgc aacatccgtc tgtggcgtag acaacatttc cgtaattgtt aataaattta
     1681 acttaaggtt gtcattttgt gacttacgac gaagattcac gcttcgggaa tattcccaac
     1741 atcaatttac catgattctg atattgacaa tcttaatgac ctgtcagact tgccataagc
     1801 cgctggtatc acaccagaag agaagcagat gacttccgga ggtttttatg gctgatttca
     1861 ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca
     1921 ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg ggcgcaattg ctgaacctgc
     1981 tagtgcatgt gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac
     2041 gggtggaaat tggtttaggt gtcggcacca ttttcgggct gatcccgttt ttagttggct
     2101 gcctcatttt tgcagtagtg gcgctatggc tgcactggcg acatcgccgc cagtaagatt
     2161 tacttgccag ataggaccta aaacgcttta tctggcatga cattcctgtt tgatcactgt
     2221 ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg
     2281 cgggtgattt tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg tagtgattta
     2341 cccgttttag cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag
     2401 aagctgtcac ctttctctaa ctgacggcga atttcgctgt cgctcatccc cagttttccc
     2461 gccagtttgc gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa
     2521 tcaccgtcca gtgccagctt cacgccgctg gccttactga ctgcgttctg aaacgcggca
     2581 ttacggctgg cgtaccagcc agcgttaaaa tcagcaaaac ggtatatcgg cgcgctatag
     2641 ctggcgggat aattcagtaa atggtaagta ccaaaccaca acccgccgcg acgactgaag
     2701 acttcctgac ggactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca
     2761 aaggcaatgc tgacctgcat cggcccaccg gtgcgcaccg gattaagcga accaaacaac
     2821 gtctgcccca ttggcaccat gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct
     2881 gtacgcaccg aatctaaccg ttcgctatag cttttgccgt ttggcgattt gatttttagc
     2941 gcggtatgca ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc
     3001 caggcgatct tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc
     3061 gccaccgcca gcaccgagca gacgttttcc agcgtcggtg ccagcccctg actttcaaac
     3121 gtggtggcga gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc
     3181 cgcacgacgc tcgccacatc tatcgctttt tcgccctttt tcaacggttg cggagcctga
     3241 ctactacacc ccactaaaac cagaaccgcc aacaatgaca gcgaagatag atgattgacg
     3301 cgcgacatta cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca
     3361 tccagctcat gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg
     3421 gtccatgagt taatcaggta ctggaaagct ccacgaacct gaccaaaaac gttggtaatc
     3481 tgcgtcatca ggccgagcgt aatcgtaccg gcgacaattg acggaaacag caagaacaaa
     3541 ccgaaaacgt tatcgacctg caaatagaga atgcgggcga tgttgaaata catatagtga
     3601 aaatagaggc ggaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc
     3661 gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt tacggtaggc agcctctaca
     3721 cgctggtttt taaactccag ccccggcagt ttgatcccta ccactgccag taatccggtc
     3781 cccatcagcg accagacgat agcggcaatc accagaccat acggaatgtg cccgacaatt
     3841 ggcagctccg gcacatgcgc ggagagcgtt accagcaccg gcaggaaggc gatcaacgtc
     3901 atgatggcgt tgataaaact gacgcccata ttctccagcg ttgaagcaaa acgcatggtg
     3961 tcttcctgca cacgctgtgc ggcaccttcg atatgacgca gttgttgcca gttcgccatg
     4021 taatattcgt tcatcgctgt acgccagcgg aacacgtagt gactgacaaa gaagttgttc
     4081 agcacactga tcaccacagc gatcagcgca atccccagaa agacgcccac ttcgcggtaa
     4141 aattgttcga tggtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac
     4201 ggcgcatacc aggcgttgac ggcgaccccg acttccacca aaaaccaggt gacgaagatg
     4261 atcagtgcag taccgagtat tgaccagtat tgccaacgat gcgggctgta gataaaccag
     4321 aacaatgcaa agagtcctac acaaacaatg tagtaagcgt aaaaaatcag gaaatccaac
     4381 gaccagaaac gcgcggcgct aatcgggatt tgcccggaag ctccggtgat acgcgccacc
     4441 caatcacccc cacctgcttg ccagaagata acggcaatca atgcccaaac aaaggccgag
     4501 agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc
     4561 ttatcgtttt aacaactgct gtgtattaac cgcatgaccg ctacaaagcg acagccaaat
     4621 gaaaccgccc attgtgaccc atagcaaggc gcttagttcg ccagcctgag cataagaatt
     4681 gtttcgacgg atttcacaaa ccaaagattg tttacataag cggattaaca cgcgcaggat
     4741 cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta
     4801 acactattat tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc
     4861 agttaccttc attcaatcta tggacaccac cgttgaaacg ttgtctgctt ttttctgccg
     4921 tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt
     4981 tcgcctctga tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg
     5041 ggatggcgct ggttgttatc gacggtaacc agcgcgtctt tcgcagttat ggcgaaacgc
     5101 gacctggtaa taacattcgc ccgcagctgg attcggtcat acgtattgct tccctcacca
     5161 agctaatgac cagtgagatg ctggtgaaat tgctcgacca ggggaccgtg aagttaaacg
     5221 atccgctaag taaatatgct ccgccaggcg cacgcgtacc gacctacaac gggacgccca
     5281 ttacgctggt gaatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg
     5341 cggcacatcg tccggtattt gtctggccaa cgcgcgagca acgctggaaa tacctttcca
     5401 ctgccaaact aaaagccgcg ccgggcagcc aggccgcgta ctccaacctc gcattcgatc
     5461 tgctggcaga tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc
     5521 agattacccg tccattgggg atgaaagaca ccacctacac cccctcgccg gatcagtgcc
     5581 gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg
     5641 gcagcggcgg agtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttgt
     5701 catctgattt ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc
     5761 gagcgcagtt tacaaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt
     5821 taggctgggt atatatggcc ccgaaagagg gtcgtccagg gattattcag aagacaggtg
     5881 gtggtggcgg tttcattacc tatatggcga tgatcccgca gaagaacatt ggcgcatttg
     5941 tggtggtcac tcgttcgcca ttgacgcgct ttaaaaatat gagcgatggc atcaatgacc
     6001 tggtgaccga actgagcggg aataaaccgt tggtcatccc cgcgtcctga ttaatactct
     6061 gaaggcaaac ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta
     6121 cgtaaagcag cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatgaata
     6181 taattcatca caccaatacg tgagcgtaat tcttcattcc agtcaatcat tgataataac
     6241 gttgcgcgga tttgttcgta ggaattatgc ccaatgagct gtacgtcacg taattccaga
     6301 attctatttt gccaggctaa ccagtaaaaa gcatctcgcc agagttgatt ttgttcaata
     6361 agggtaatgg tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat
     6421 ttgtatggta tatcgttttt cattaaacca tcagccagtc ccataatata aggagcctgg
     6481 gtaataccga taagtacgtt ttcttctcta cgcagagaga taacgccctc taaaataacg
     6541 aaagtatctt cgctattgac atcacttgaa aatataactt gcttttcatt attaaactcg
     6601 aagcgcgtgc catatctgga caaacattta tcgagcttac caaattcctg aagaggttta
     6661 actacagata acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt
     6721 atttagatta aataatctat ttcttcagag tgaagaatat taccaggtat atttaacacc
     6781 cacgttcgcg gaccagtctt gatctacgtc accaccaccg aggtagttag catcggtata
     6841 ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta ccgcagaccc
     6901 ttcagtaccg ttattgatgg aatcaccgtt cacatcgtta tcgttgttag agtcgtcgta
     6961 gacgtaagcc agtttgaagt acggagtcag agcctggtct tcgctgtagg tgaaggtata
     7021 acctgcatct acacccagtt cataacgcat gctgtcgtaa gactgaccgt caactttcat
     7081 gtcgttgctc agctggtagt catcaccaga ctggaacaga ccagaaacgc tgccgtaagg
     7141 agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca
     7201 agcgtcggag ttggtgctac cgtcaacgta agtaccgttg ctcatggttg cagacaggtc
     7261 gttgttgaag tgagagtagc ttaagctacc atcaacaaag acgttgttcg cgaagtgagc
     7321 agaagagtag atgtaggcag tctggctgtc ttgatccacc tgaccagtac ggtcattcat
     7381 gtcacctttc gcgaagccag cagccgcacc gacgatccac ttagcgttgt taccgtcaat
     7441 tttggtatca acaccgacca tgatgccgtt aacatcctga tcatagttga tggtgccgtt
     7501 gtcgccgttg aagttaccac cgaagtagct tacccatgcg ccgccgttat cagccaggcc
     7561 atgacgagag ttggtcagac gagtaccaac ggtgtcttgt tccaggttcc agatattggt
     7621 gttcgcagac gggatgctca gcgccatgtt agcgtagtcg gtcagctcca tctgttgcag
     7681 aacaacggtg ttaccacgct gttcagcctg ataggtgtat gcacccaggt cagctttgtt
     7741 agcagcagag aaggtcgcgt tgctgttttt gtcgttaacg tagatcagct cgttgccgtt
     7801 gtagtcagcg atagaaccag cacctgtagc gttgtcgata cgaactttgt agttaccggt
     7861 tgcagctgca acatggtcac cgttgttatc aacttcgttc tgaccagctt cgatagaagc
     7921 agtgttgtcg ttatcgccgt taccgttaat ggtcaggtga ccatcagagt tcattgcgat
     7981 taagccataa ccgtagtttg acttggtagt atcgttggta cggtcgttgg tcaggtcggc
     8041 atcaagtacg aagcggttgc tttcgatgtt gaacacacca gcgtgaatgt taccactagt
     8101 atcagtagtg ctggtcaggt tcagagtatc aacctgcaga gcattcgccg cagtgttacc
     8161 gacattcaga ataccgtcgt tagtcacttt gatgttgttg gcatacagag cagcatcacc
     8221 tacagcccaa ccagcagaaa cttcatcaga aatggttacg gtactgttgt cgacagtcag
     8281 tgaatcagtt gcaacatgac catcttcgtt gatgttcagc gcagaaagac ctgtcagaga
     8341 gatagagtca gaaatcagcg ttgaatcagc aacattaacc gttgaaccac tgttgatgct
     8401 cagagtatca atcagagaag ttttggtggt atcccactgt gaaccggtgt tcagggttac
     8461 attgaacaga ccgctctggt aaacttcgtt accgatgatg tgatcaaatt cgctatattc
     8521 agagtcgctg ttttctacac caacagttga tgaaggccac aggctgttag ctgcgatgtc
     8581 gatcagagtt gcagtggctt cagtattgat tccatatgta taggagtcat ttacaccatc
     8641 gtcatcagta tcaacaccaa tctgatgcgc agacattgcc gcaccgaccc acttgctgcc
     8701 attgttcagg gtcagatcca ggcgatcagt accatcccaa ccgttagtat ctacatcacc
     8761 gtcagcatca cgatagctat cacgaccatg cgggaagaag ttctcatcaa agttacttga
     8821 gaagaataca tcgcccatca gtgtggaatt atcgaatgtt gcagtggttt gcatggcgtt
     8881 atcagcagca ggatgtgcaa taacagctaa tgcgatatcg tcagcattcc aacctttacc
     8941 attgtaatcg ctggcattac ctgtattacc gaaccaacca gttgtacctt catcagtcca
     9001 tgaaccagag gtcacggttg aatctttaac agtgatggtg ttattaaaca cttcgctgct
     9061 gtttacacca gtgctgacag tgttgtcgtc gatatcttcc cactcataac cctgagtcag
     9121 cgtaatacct gctacatgag agttattaga gatggacaga tcaacttcct gatccagagt
     9181 gatggcagta ccaatgttaa atacatttac aacatgagtg tcagcggcat tattgtaggt
     9241 gccgttatat gtgtagtgtt cgtagttgtc atcgatcgtt gaattatcaa cggtcagcgc
     9301 cagacgatca tggtagtagt catccgcacg atcagcacaa tcagttgtca tgcactcgga
     9361 ataaatcatg ccgtgaatag tgctgttttt gattgtcagg ctattggcgt tggtattagt
     9421 agaaataccg tcatccagat agtaggtaga aatcacgccg ttaacggtag cgttattaat
     9481 gaccggatag atatcgccgt tgtaataact atcagcggca tagttattcc agcctacata
     9541 accatcatag taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta
     9601 agtttgacca gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca
     9661 agccaatgct aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc
     9721 ctcagggacg taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc
     9781 gcgttgacga ttatgcggcg ttatagagga atggttcaat tattctttcc cctaagtccg
     9841 attgcggaca tatataaagc acgtgaacac acacacagaa agaaataaca aaaacacaat
     9901 atttcaatac gttaatgaaa gcgttaattt taaaacatag accatgaaaa tataaaacaa
//
LOCUS       DARNVM010000179         9176 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.179, whole genome shotgun sequence.
ACCESSION   DARNVM010000179 DARNVM010000000
VERSION     DARNVM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.179"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(33..299)
                     /locus_tag="SI621_004058"
     CDS             complement(33..299)
                     /locus_tag="SI621_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HEI4066711.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDSEASTRQ"
     gene            complement(296..1063)
                     /locus_tag="SI621_004059"
     CDS             complement(296..1063)
                     /locus_tag="SI621_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066712.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVRPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRKRR"
     gene            complement(1073..2224)
                     /locus_tag="SI621_004060"
     CDS             complement(1073..2224)
                     /locus_tag="SI621_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HEI4066713.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVEDDASRSLWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTAHVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            complement(2340..3620)
                     /locus_tag="SI621_004061"
     CDS             complement(2340..3620)
                     /locus_tag="SI621_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEI4066714.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADVVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3661..4893)
                     /gene="mdtM"
                     /locus_tag="SI621_004062"
     CDS             complement(3661..4893)
                     /gene="mdtM"
                     /locus_tag="SI621_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HEI4066715.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGIIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5371..6327
                     /locus_tag="SI621_004063"
                     /pseudo
     CDS             5371..6327
                     /locus_tag="SI621_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(6570..7982)
                     /locus_tag="SI621_004064"
     CDS             complement(6570..7982)
                     /locus_tag="SI621_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEI4066716.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8159..8323
                     /locus_tag="SI621_004065"
     CDS             8159..8323
                     /locus_tag="SI621_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEI4066717.1"
                     /translation="MEFHENKAKAPFIGLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8420..>9176
                     /locus_tag="SI621_004066"
     CDS             8420..>9176
                     /locus_tag="SI621_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEI4066718.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVAFGQQGYS"
BASE COUNT         2377 a         2385 c         2330 g         2084 t
ORIGIN      
        1 agccaaatat ccgggcttta tatttaaagg tcttattgcc tggttgacgc ttctgaatcg
       61 taatgcacca cacactgcca gtcgctgccc tgctggcaat atatcgcttc ggtttgccca
      121 gggatcaccc attgtatttc ctcgccgggg ggacaggtca gccaaatgca taacccgcag
      181 ccgccgcgta aatcacgggg aatatcgctg acgcgaaaag tcatgcccgc tgcctgcaac
      241 gccttacggg tttgtatgac gccgaccgtg gagtgaaata aaaataaaaa ctctttcatc
      301 ggcgttttct cactcgttga ccaattaccg ctgcgccaat tgcgccagca aattgcgcat
      361 caggatgggt atttaccggc attcccagat gagattccag catccgggca aacgtctggc
      421 aatgactaac gccaccagta aacagaatcg gcgcttcaca ggagagacga gcaatgaaat
      481 tggcactccg ccgcgccatc gcgttaatca ctccggcgag aatcgcttct ggcgcgacgc
      541 ccgctgagcg caggctgatc acttctgatt cggcaaacac tgtgcacata ctcgtgatgg
      601 cgtgcggtct gacattttca gtaatgctgt cgagttgctc gacgctggtg ccaagcgtgc
      661 gcgagatcac ttccaggaaa cgcccggtgc ccgccgcgca tttgtcattc atcaggaaat
      721 cgcacaggtt accgtcatca tcaagctgaa tcactttgct gtcctgacca ccgatgtcga
      781 ttaccgcgcg cgttgctggc gcaagaaacc gtgcgcccag cccgtgacag gagatttccg
      841 tcacctgttt atcggcaaaa tccaccagtt gccgtccgta gccggtgagc gtcagaaacg
      901 gcgttgtttc taacccttcg cgcagagttt cccaggcttc agtaattgct gttgccgggc
      961 gaaagggggt tggaacgagg aaacggcgcg taatcacgcc gtctgccagt aagatccctt
     1021 tggtggctgt agaaccagaa tcaatgccaa tcgaatatgt cactgccact ccttacagca
     1081 tctcaataaa ggccgcgaca cgggtgctga gctgcccgat atctgaggtg gagtagtctg
     1141 tttcaatagc gatataagga atgttgtgct gatgacgcac atgacgttta atcgccagcg
     1201 attccaccgc gtaggtatgg cacgcctgca aaatcacatc aactacgcca tcgacctgat
     1261 attcttccac catctggctg agcatttgca ggcgctgatc gttcggcgaa acacaggagc
     1321 agccaatcgc cagatattta tccgccagcg cgtcgtagac atcgcccgtt tctgccacgc
     1381 attgctcggt cgctttcgcc ccggtgcagt tttcataacc gacaacccag ccgccattct
     1441 cttcaatcgc gcgcaccact ttttctgctg cgccgccaat cgggcagccg gtgattaaaa
     1501 tgcgcggacg cgggtccagt cgctggcctt cttcccactg ctgacgaacg tgggcggtca
     1561 ttgcatccag ttcattgatc aacgcctctt tatcgaaccg gaaggttgcg ccgtaaacca
     1621 ctttcagaat gtcgctgccg ctaagcgccg gaggatttaa ctgcccaaga tgataaaaat
     1681 tagccagtgc gcgacgttcg cggtttttca gcgcaatggc atcgcgcaga gcatcttcgc
     1741 taatttcgtg cccaaaacgt tcttctaccg ttttttgcaa gcgcagcatc tcggctttcc
     1801 ataacgagcg tgaggcatcg tcctcaacgc tgtttggcaa ctgcatcaca tgaacgggct
     1861 taaactccgc catgtattca tacattttct ttttgccgtc gcaggtggtt tcaccgacca
     1921 ccagatcaga aaagtagaag taggggcatt tatcggtttt gccgaagccg tagctgcttt
     1981 taatcagcgg gcagaggttg cgcggcagat ctttctccgc ttcttcaatg gtttcatcag
     2041 aggtggaaca gagcgaaacc acaactgcac cggctgccat cgggatctct tgcggcataa
     2101 aggtgcagta ggtgccaacc agcggaatgc cgcgctcctt gagatccatg acggtgagaa
     2161 agcctttctg gcgagcttca gagaactgat caaaaatggc gggtagatcg gtgacaagtg
     2221 acatgatttt ccttccccgt accacgggag ataatgaaaa gaggccacat tattaccatt
     2281 cttttagtgt ggtttatttg atctctctcg cggttagcca cttagttttt catggatttc
     2341 taaaaattgc cgagggggaa caaggccggg agctgcgaaa gcaaatacaa aattagccca
     2401 atacaaccgc caaccagcgt accgttgaca cggataaact gcagatcttt gccgatattt
     2461 aactcgattt gccgcgacat atcctgcgca tcccagcttt ttaccgtatc gctgatatgt
     2521 cgcgtcagga atgcggaaaa ctcaggcgcg acgcggtgcg cggcttgttc cagatgacca
     2581 tttaacgacg cccgcaaggc atcatcggca attaacgttt cgccaaacca ttgccccgcc
     2641 cgcgcgatac gttctttcac gcgagaatct tcactgttga tgtccgcttt cagccattcc
     2701 cgcaaatccc cccacaattc actgagatag cggttaaaag cttcgtcttc tttcagatag
     2761 ctttttacga catcggctcg cgctgccatt tcaggatcgt ttttcagctt gtcgatcagg
     2821 gcaaaggtgg cgcgatcaaa cgcatggcgg atctgatgcg cacgatcgcg gctgatatca
     2881 tcaagcaaag aattcaccgc gtcagaaacc aactccgcgc tatgttcgcc cagccattca
     2941 gtggggagaa ttttggcttt cagtggatgc tcactctcca gccagcgaac aatctgctgg
     3001 gcgataaact tgcgcgattt atcgcgctgg agaagggcga tcaactgtgc gatcagcgta
     3061 tccagcagca cctgatgacg atcgtttttg gtcatactct ccagcatcaa cgcactggtg
     3121 ccggaaagat cgaccttatc aatcgcccga tggactgcgc gcttaagcag gcgctgaata
     3181 cgcgcatcat cggtcagttc aagaaaaccg ctcatgatct gcaacagatg ctgaccaacg
     3241 cggcgggcgt tttctggctg actaaaccag ttgccaatca acaacgccgg ttcgtgacgt
     3301 cgaatcaatg ccaccagcga ttgggtatcg agaaattttt cctgcacgaa ctgaccgaga
     3361 ttttcgccaa tccggtcttt attacgcggg ataatcgccg tatgacgaga aatgatcgga
     3421 atcggcacgc ggcgaaacag cgccaccacc gcaaaccagt ccgccagcgc gccgaccatc
     3481 gccgcttcag caatcgcctt cacgccgctc acccaaaaat tgggcggcaa aaacagcgta
     3541 acgacaaagg tagcagcggc gataagcaat aaagagagcg ccaacatttt ggcgcgtctg
     3601 agttcaatga gtttattcat aacgttaaga atagagcctg acggtaggat cgcgcaaaaa
     3661 tcaccgctcc tccgccagct cggcagcctg atgttggcgc acgcgattga gcaatcccgc
     3721 cagggtgaaa acgacgataa tgcccgccac gacggctaac agatgaaacg gcaagcgacc
     3781 gccgttaaac cacagccagc ggccgatttc gactgagaca gacatcacca tcaggatcac
     3841 catattcagc gatgcggaga cggttccttt cggtaagtta ttggaaaaca gcgtaaagcg
     3901 gaataaggtc gggaagatca aaccaatccc gaaagcatac aaactggtgc ccagcaccga
     3961 ccacagccag acgtgcggtg acagcagatt gccgataatc aacagcgcga ggccaaccag
     4021 ttgaaggggt acggcacgcc agataaagcg cggttcggtc ggatctttaa cgaaacgcgc
     4081 cacgatggca ttcgcgacaa tcaccgcgcc gaacaccgga acctgtgtcc aggcgaactg
     4141 cgaagttgtt aagccgcccg cgtcgatcag gatcaccggc gagacagcca cccaactcat
     4201 catggggata tagcttaaag agatggttgc tgcgccaaag aggaacagcc gattgcaaaa
     4261 gacattacga aaatcgcgca agacgctttt ggcgctaaat ggaaccgcgc cgcgcttcac
     4321 cgtctctggc atcgccagca gtaagccaac aaatgagata aaacccataa ccgcaatgat
     4381 ggcaaaaagg actttccagt gcacaaagtg catcagagct gcgccggaaa gcgggccgat
     4441 aatcggcgca atcagtacga tggaggtgat aatcgccatc aacttgatcc cttttgtctg
     4501 tccgaacgcc tcctgcaccg tgacataacc aacggtggca ataaaacaga tactggtgcc
     4561 ctgaattgca cgcgcgataa gaaattgtgt cattgacgtg gtgaacattg tcgcggcgca
     4621 ggcaagggta aaaattaacg ccccggtaat cagcaccggc ctgcggccaa ttctgtcgga
     4681 aagcggcccc agcagccact gcaatgccat gcctccagcg agatagagac tgacggcagc
     4741 cggggccaga ctgacatcgg cattaaaatc acggaccaca ttaatgatcc ctggctggat
     4801 caggtccgtc gacagatacg cagcaaagtc atacaaaatc aacgccatcg ggaaaaacag
     4861 cgtggcggca tggcgagcaa aaaaacgtgg cataacaaac aactccttgt ccggagaacc
     4921 ccaacgggga aaacgaaatt caataccgtc ggtaatgacg aaaggcaggc gagaagggca
     4981 acgagactgc aaatgtactg aatttcatgt aatcaaccac actgcctgtg aacacacaga
     5041 cagagaaata ccttctgact tcgggtcaga atgtataacg attacaaata gtcatcagca
     5101 cattacacct cttgaattga tgggttgcat agaaaatata caccttaagt gtaattaaaa
     5161 tttgcggtta atcaaaagag tgcggtggaa aggggaatat ctgccgggta caggaaatgt
     5221 aaaacgcgag cctgtttccg gaaaaatgta tcgtttgcag aggaacaaaa atagcctgga
     5281 aagcgcctcg gggaatggga aattgccggg tgagaatggt tttgttagtc gctaaagtcg
     5341 ggccatctta tcaacaggtg acggatcgcc atgacaaact tcacgaccag cacaccgcac
     5401 gatgcactat ttaaatcttt tctcacacac cctgacaccg cgcgggattt tatggagatc
     5461 cacttaccaa tagatttacg tgaattgtgc gatctcgaca ccttaaaact ggaatccgcc
     5521 agttttgtcg atgaaaaatt gcgggcgcta cactctgata ttctgtggtc ggtaaagacc
     5581 cgttaaggcg atggctatat ttacgtagtg attgaacatc agagccgcga ggatatccat
     5641 atggcctttc gcctgatgcg atattccatg gcggtgatgc agcgccatat cgagcatgat
     5701 aaacgccggc cgctaccgct ggtcatcccg atgctgtttt atcacggtag ccgtagtcct
     5761 tatccctggt ccctgtgctg gctggacaaa tttgctgacc cgaccaccgc acggaagctt
     5821 tataccgcag cgttcccgct ggtggatgtc actgtcgtgc cagacgacga gattgtgcag
     5881 catcgcagag tcgccctgtt ggagttgatc caaaagcata ttcgccagcg cgatctgatg
     5941 gggcttatcg atcaactggt actattactg gttacagagt gtgctaatga cagccagata
     6001 accgcgctgt taaattacat tttactgact ggcgatgaag cgcgttttaa agagtttatc
     6061 agcgaactaa ccagtcgaat gccacaacaa agggagcgaa taatgacgat tgcagagcga
     6121 attcataatg atggatggct gttgggaagg gagaggggga ggaaagaagg gaaagtagaa
     6181 ggggaacgga gcctcctccg attgttgttg cagaatgggg cagatcctga atggatacaa
     6241 aagattaccg gactctcggc agagcaaatg caggcattag agcagccctt gcctgaaagc
     6301 aagcgcgatc cttggatcga gtactaatca gagacttatg acaaacgcaa agctgcctgg
     6361 tgcgctacgc ttatcaagtc tacataaccc attcaatata ttgaacttta aagattttgt
     6421 agacctggta aggcgttcac gccgcatccg gcatgaacga agcacacttt gcctgagcca
     6481 tgccagaacg tggcgatgtc gctataaaga taaaacgagt tacaggcgat tcgtgagagt
     6541 aaatcaggcg cttcgccact atttcgaatt cattccattg cccgatacac tgcctcgcca
     6601 atttgcttca gtgcttcgcg ataggtttcg ctgagcggca aagcgcagtt gatgcgcaga
     6661 caattacggt atttgccgga agctgagaaa atcgagcctg ccgccacctg gattttcatg
     6721 cggcacagct gccgcgcgac gcagaccata tcgacctgtt caggcaattc tatccacagt
     6781 aaaaatccgc ctttcgggcg cgtaatacag atttcgcagg gaaaatattc ccgtatccag
     6841 caggtataaa gcgccaaatt gcgctgataa gtctgccgca tccgccggat atggcgatga
     6901 tagtgacctt ccagcacaaa cgttgccgcc gccatttgcg tggacggtac attaaagctg
     6961 ctgatggcgt atttcatatg catcagttta tcgtgataac gtcccggcgc gacccagccc
     7021 acacgcaggc caggtgcaat acttttactg aacgagctgc acaacagcac tcgcccgtcg
     7081 atatcccagg aatgaatggt ccgcgggcgc ggatactccg ttgccagttc gccatagaca
     7141 tcatcttcaa aaatcacaat atcatgacgc tgagcgagag agagaacggc ccgtttgcgc
     7201 gcgtccggca taataaatcc cagcggatta ttacagtttg gcaccagaat gatgccttta
     7261 atcggccact gttccagcgc cagttccagc gcttcaacgc tgatgcctgt ttctggatcg
     7321 gttgggattt caatcacttt cacgcccatg ccacgcagca tctgcatcga accgtaataa
     7381 cagggggatt cgaccgcgac aatatcgccc ggtttacaca ccgccattaa cgccagcgac
     7441 atcgagttat ggcagccgct ggtaatgatg atgtcatcgg cggtgaccac cgagccgctg
     7501 tcgagcatca ggcgggcaat ctgctctcgc aacactcgct gaccggctaa caagtcataa
     7561 ccgagaacgg tttgcagatt atgctgcacc acccggctta gctcccgcca cagcggtttc
     7621 aggctgggcg tttcgacatc cggcgtgctt ttacttaacg gaacaatgga actgtcgcta
     7681 tgcgcctcca gcatatccag cacctgatcc cactgggtaa tttccaccgg gcgctgcacc
     7741 ggacgcgtca tcggcggcac tggcggctgg gctttacgtt gtgcgacaaa ataacccgaa
     7801 cgcggctgcg gagtgatgag cttcatcgtc tccagcgtct ggtacgcctg ctgcacggtg
     7861 ctaatactga cgccgtgctc ctgacttaag ctgcgcaccg acggcaattt ctccccgtga
     7921 cgatacagcc cttgctcaat ccgctcggca agcagagtcg ccagatgttg ataacgcgtc
     7981 atgctgtatt ccttctttgg accatacaga gagaaaaacc ggtacagatg aggctaaaaa
     8041 accggatgca gatgctgttt taacctgtct gtatgttaaa taaaactaat atttgcatct
     8101 gtattgtgaa ctgccagaag acggatgatg aagcctctga aagatgagga ggcagtcgat
     8161 ggaatttcat gaaaacaaag ctaaagcacc gtttattgga cttgtgcaac tctggcaggc
     8221 agtgaggcgt tggcggcggc aaatgcaggc ccggcgggtg ttacagcaga tgagtgatga
     8281 gcggttgagg gatattgggt tacgcaggga ggatgtggag tgagggggct gttcgtcgcg
     8341 gataaacagg atttctctat aacctccaca aggaagtggt tattattgcg aaatcatcgc
     8401 ttcacggaaa gacgtcagaa tgaaagctac agaagcccgc ctactcgatt ttttaaaacg
     8461 ttcgcagcag tttgttattc ccatttatca acggacttat tcatggacag aacaacaatg
     8521 tcggcaactt tgggacgaca tcattcgtgc cggaaagcgt gacgatatat cagcgcattt
     8581 tatcggttcg gttgtttata tagagcaggg attgtatcag gtttctggta tttctccgtt
     8641 gctggtcatt gatggtcagc agcggctgac gaccgcaatg ttgctgattg aggctttatc
     8701 gcgccatctt ggcgaagacg aagtttttga tggcttttcg gcaatgaaat tgcgtaatta
     8761 ttatttgctc aatccttatg agtccggcga gaaaggattt aaattactgc tgaccgagac
     8821 tgataaagac agtttgctgg cgttaataaa acaaagacca atgccagaaa actattccca
     8881 tcgaataatg gaaaacttta ctttctttga tgaacaaatt gccaaactcg gtgatgactt
     8941 gatcccctta tgtcgtgggt tagcaaagtt attaattgtc gatgtggcgc ttaatcgtgg
     9001 tcaggataat ccgcaactga tttttgaaag tatgaactcc accggtaagg cgttaagtca
     9061 ggccgatctg gtgcgcaatt ttattctgat gggcctcgaa ccagagcatc aaacccggtt
     9121 gtatgaagat cactggcgtc caatggaagt cgcttttggt cagcaaggtt acagcg
//
LOCUS       DARNVM010000180         9158 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.180, whole genome shotgun sequence.
ACCESSION   DARNVM010000180 DARNVM010000000
VERSION     DARNVM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.180"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..202)
                     /locus_tag="SI621_004067"
     CDS             complement(<1..202)
                     /locus_tag="SI621_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HEI4066719.1"
                     /translation="MKTITVLLTTVLFSMSASAALTPACEEYYKEIDNFVAKMKEMGT
                     PEAQVNTLKQQYEQSRKQIAALP"
     gene            648..1642
                     /locus_tag="SI621_004068"
                     /pseudo
     CDS             648..1642
                     /locus_tag="SI621_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1688..3060
                     /locus_tag="SI621_004069"
                     /pseudo
     CDS             1688..3060
                     /locus_tag="SI621_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(3217..3930)
                     /locus_tag="SI621_004070"
     CDS             complement(3217..3930)
                     /locus_tag="SI621_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEI4066720.1"
                     /translation="MNTLSFLLFSEEESLFITLGKICVAFILGGIIGLERESKGKPVG
                     FKTCVIISVASCVLTIVSIQSAEYYAEISMNIRSDPMRLAAQIISGVGFLGAGVILHR
                     HDDAISGLTTAAIVWASAGVGITCGAGFYMHALVTTALFMIAIKLSYFVLFLQAKNQF
                     PGKVKVHVTLDERSGLQALIECINQQKNIIEAFTIRDVKKGRIEVNLKVVIRKKMTLP
                     ELYHSLSKLEHVCAIALEH"
     gene            complement(3994..5013)
                     /locus_tag="SI621_004071"
     CDS             complement(3994..5013)
                     /locus_tag="SI621_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEI4066721.1"
                     /translation="MKFNPRKSENESMRIIIDCDPGNGIPGANIDDGLALALAIAAPQ
                     IALEMITTVAGNTPVDVGYAVARDLITQLDIPVAVYRGASRALLEDPQPWREKLDHGV
                     DQFGLRQLWSNVPAPALCQQVEPHAPEAIGELICRNPGEITLVATGPLTNVAIALQLY
                     PQIVHAVKNIVVMGGVFNVPGYLKDTNFGLDPEAAHAVLTSGAPVTLVPMDVTTQTQM
                     LHADLDRLAKTENGLSRYLAQTIRPWITYSMQTRNLPGCWIHDVLTIAWLLDPSLATT
                     AEDYLDVSLEGITRGMTCCYGRDTLRLNIGIPEPKGAQVTILQSIDNPRLISLIEHYI
                     QNYGA"
     gene            complement(4988..6019)
                     /locus_tag="SI621_004072"
     CDS             complement(4988..6019)
                     /locus_tag="SI621_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEI4066722.1"
                     /translation="MTQSHSQRVTRSDVAKEAGTSVAVVSYVINNGPRPVAEATRQRV
                     LQAIKKTGYRPNGIARALASGSTQTYGLVAPDISNPFIASMAHALQHEAFADGKVLLL
                     GDAGDSSCRERELINNMLHRQVDGLIYTSVDRHPYINLIQESGTPCVMLDRVDPGLNV
                     SVIQVDEQLAAMQVTQHLIDHGYRDIGIICGPREMLNTQDRIRGWQQALEASSLVVNP
                     SWIFSTNYTRAGGYEATKRMLEHQLPRALFATNEQQALGCLRALAEHGLRVPEDVALV
                     CFNATQESAYNVPSLTAVRQPVDKMARAAIDMLKNWDGEVRRVEFEFYLRTGESCGCQ
                     GHEVQPEKK"
     gene            complement(6124..7518)
                     /locus_tag="SI621_004073"
     CDS             complement(6124..7518)
                     /locus_tag="SI621_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HEI4066723.1"
                     /translation="MNTHSSTVSLTDNQQAAQERLETSEGRREFWRATVSCWLGTTME
                     YADFALYGLAAGIIFGDVFFPEATPVMALLSSFAAYSVGFIARPIGALLFGWIGDKHG
                     RKIVMVITIGLMGMSTMLIGLIPSYAQIGVWAPICLVILRFSQGLGAGAELSGGTVML
                     GEYAPVKRRGLVSSVIGLGSNSGTLLASLVWLIVLQMDKDDLLSWGWRIPFLCSILIA
                     AAALLIRRHIRETPVFERQKALLQAEREKVIREEKAQQQHDSRSFWKRTRAFWTMVGL
                     RIGENGPSYLAQGFIIGYVAKVLMVDKSVPTAAVLIASVLGFAIIPLAGWLSDRFGRR
                     IIYRWFCLLLILYAFPAFMLLDSREPWIVIPTIITGMGLASLGIFGVQAAWGVELFGV
                     TNRYTKMAFAKELGSILSGGTAPLIASALLSYYGHWWPIAIYFAFMAAIGLVTTFFAP
                     ETRGRDLNLPEDAI"
     gene            8517..8785
                     /locus_tag="SI621_004074"
                     /pseudo
     CDS             8517..8785
                     /locus_tag="SI621_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8769..8903)
                     /locus_tag="SI621_004075"
     CDS             complement(8769..8903)
                     /locus_tag="SI621_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4066724.1"
                     /translation="MKIVQTSLSESVTQHLAERILCGELPAGSVLPGENELATVSRGG
                     "
BASE COUNT         2524 a         2197 c         2211 g         2226 t
ORIGIN      
        1 caggtaaagc tgcaatttgt tttcgggatt gttcatattg ctgtttcagc gtattaacct
       61 gtgcttctgg tgttcccatt tccttcatct ttgctacaaa attatcgatt tctttataat
      121 actcttcaca tgccggagtc agtgctgcac tggctgacat actgaataat actgtagtaa
      181 gtaagactgt aattgttttc ataaatacac tccttttttg tacaaaaaca ctcaaccttc
      241 ctgagaaggc cataaaagca gaatgttatt ggaatatcag caaaacgagt attcccacag
      301 gttttttctt ggtatcagaa ttctgggata taaatccagt aaatgatgga gtaagtccga
      361 aagaatatgt ggtgtgatta cggactacca gtaaatatta ctgatttcat gccatgaaaa
      421 tactcattat aaaaagcgcc tgtatgctgc ttataaaata tcagaatccg atgtgtcaaa
      481 atgtgatata tattaaaaaa taaataagac tgacccaata taccaaaaga ggatgacaga
      541 agtgaaggat aaccgtatta ttctggacca gaaaggagcc tgtacataga tttgtgtaat
      601 tgcctgattt tgatatgttc aattcacctt caaatgaagg tgaatttatg gacgacaaac
      661 aattgcaggc tctggctaac gaactggcca aaaatctcaa aactcctgaa gatctcagtc
      721 agttcgatcg cctgctcaag aaaatcaaag ttgaggctgc tctcaacgct gaactgactc
      781 accatctggg acatgaaaaa aatcaaccca aatcgggaac caactcccgt aatggctatt
      841 ccacgaaaac cgtcccacta ccgacgggcc gatggaatta cgtactccac gtgatcgtga
      901 tggttccttc gaaccacaac tggtgaagaa aaataagacc tgcattatcg gcatgaacaa
      961 ccagatcctg gcgctgtatg ccagagggat gactactcgc gaaattacct cagtcttcaa
     1021 agaaatgtac gatgcagacg tttctccggc gctgatatcg aaaatcaccg atgccgtaat
     1081 cgaccaggtt gtagaatggc aaaaccgtcc tctggatgcg atttatccca ttgtttatct
     1141 tgactgtatt gtcctgaaag tacggcagga cagccgggtt atcagcaaag cggtgttcct
     1201 ggctctgggt attaaccttg atggtcagaa agagctgcca ggtatgtggc tggccgaaaa
     1261 tgaaggtgca aagttctggc tgaatgtact gacggaactg aaaaatcgcg gcctgaacga
     1321 tatccttatc gcctgcgtgg acggcctgaa aggcttcccg gaagcgatta atgccgtcta
     1381 cccggagacg cagctccagt tgtgcatagt acatatggta cgcaacagcc agcggttagt
     1441 ggcgtggaaa gactataaag cggtaacccg tgacctgaaa caggtttacc acacagccac
     1501 ggaagaaggc gcgttgcagg cgctggatgc gttcgggaag gaatgggatg cacggtaccc
     1561 gcagataagt cgcagctggc aggcaaactg gccgaatgtt gccaccttct tcgtgtaccc
     1621 ggaggcgagc cgcaaggtta taataaataa ccgtcaatac ggtgctccgt taacgcttac
     1681 gtctgttatg gtactttctg attgttattc atgggctaac gagcagtttg gacatgccag
     1741 tctcggtgat ccgcgtcgta cacgacgcct tgtgtcactg gcctcttctc tcgctcagca
     1801 tgcaggtctt tctatcgtga aatcatccca gtccactgct caggttgaag gtgcgtaccg
     1861 cctgatgcgt aacccgtcag tgtctccgga agccattgcc gaagccggat ttaccgccac
     1921 tgcaagggca tgtgaggcac atccgctgct cctcgcactg gaagacacca ccaccattag
     1981 tttcagccac agtacggcat ttgatgacct agggaatacc actactaacc cgaagacgcg
     2041 cgggctgctg gctcactctg tcctgatgta tgcccccgac agcgccctgc cagtcggtct
     2101 tatcgaacag cagcgctgga gtcgggccac ggacacctac ggtgttaagc accagcgaaa
     2161 agaacgtccc tgtgaagaaa aagaaagtta tcgctggcag caggcttcgg agcgtatggc
     2221 tgaacggctg ggggagatac agaaacgggt aatcactgtc tgtggcctgg aagcggatat
     2281 ctggcattac ctgcattata aagtcagcca cggacagcgc ttcgtggtgc gtgctgcaca
     2341 gaatagccgg caggaagaag cacccggcaa gctcttcgaa ctgccggaag tcctgacaac
     2401 cgcatgaagc cacatgctga atgtgatgta aaaaggcggg cgcgcagccc gccaggcccg
     2461 gatgttcacc agttacagcg aagtcggcat aaaaaatccc gacaacagcg gccaggcgct
     2521 tccgctcacg tatgtctgtt gccgggagca ggcagaggac ggtgcctgct ggcatctgct
     2581 gacatcagaa aaagtggcga gtgccgccga tgcgcgacgt atcgtcagcc attacgagcg
     2641 acgctggttg atagaggaat accacaaggc gtggaaaagt ggtggtacat gtgtggaatc
     2701 gctgcgtatg cagacccggg ataaccttga gcgcatagtg gttatcaagg cgtttatcgc
     2761 ggtacgggtg ctgggcctgc ggcagggagg tatcagtgaa gaaacacaaa atgacagctg
     2821 tgagaaaata ctgttgccga cagagtggaa actgctgtgg gttaagctgg aaggaaaacc
     2881 gcttccgtca cagacgccga cactgaaatg ggcctgtctg aaactggcga aactgaggcg
     2941 atggcatgac agcaaacgca caggtcgccc cggttgtgta gtcatgtgga tggctggttc
     3001 agacttcagg atatggttga gggttacctg gtgatggagt ctcttgatca ggagctctga
     3061 tcaagagaca gctagtacag tagggggtac cattatttaa ttcctgcagt accaattaac
     3121 acagagcatt ctgcctctgt gaacatatga tatccagact tgactaattg ttattgtgaa
     3181 tgtttttatt gctgttttag cctctaatcg atgctttcag tgttccagcg cgatagcgca
     3241 aacatgttca agcttagaga gtgaatggta tagttctggt aatgtcattt tttttctaat
     3301 cacgacctta aggttaactt ctattcttcc ctttttaaca tcgcgaatag tgaacgcttc
     3361 aataatattt ttttgttgat taatacactc tatcagggct tgtagccctg atctttcgtc
     3421 aagagtaaca tgaactttta ctttacccgg aaattgattc ttagcttgca aaaagagcac
     3481 aaaatagctg agttttattg caatcatgaa caaagcagtg gtaaccaaag catgcatata
     3541 aaacccagca ccacatgtaa ttcccacccc agccgaagcc caaacgatag ctgcagttgt
     3601 tagtccagat atagcatcgt catggcgatg aaggataaca cctgcgccga gaaaacctac
     3661 accgctgata atctgtgctg ccagtcgcat cggatcgcta cgtatattca tcgagatttc
     3721 agcgtaatat tctgcagact gaatcgatac aattgtcaga acacaactgg cgactgaaat
     3781 aatgacgcac gttttaaatc caacaggctt ccctttcgat tctctttcaa ggccaataat
     3841 gccaccaagg ataaacgcga cgcaaatttt gcccagtgta ataaagagag attcttcttc
     3901 actgaataat aagaaggata acgtattcat atttcctccg gaaaaaactc actccaaact
     3961 gtgtacgaag gtctcctttt ctgccccatg aggctacgcg ccgtagttct ggatatagtg
     4021 ctctatcagc gaaataagcc gcgggttatc gatgctctgc agaattgtga cctgtgcacc
     4081 ttttggttca gggatcccaa tattgaggcg taatgtgtca cgtccatagc aacaagtcat
     4141 tccgcgtgta atgccttcca gagatacatc cagataatct tcagccgttg ttgcaagaga
     4201 gggatccagt aaccaggcaa tggttaacac atcgtggatc caacacccag gcagattgcg
     4261 ggtttgcata gagtatgtaa tccatggtcg aatggtttgt gccaaataac ggctaagccc
     4321 gttttctgtt tttgctagac gatccaaatc ggcgtgaagc atttgggttt gcgttgtcac
     4381 atccatcggg accagcgtga ctggcgcacc gctggtgagc accgcatgag ccgcctcagg
     4441 gtccagacca aaatttgtat ctttcaggta gcctggaaca ttgaacacgc cacccataac
     4501 gacgatattt ttgaccgcgt gaacaatctg cgggtaaagc tgcagggcga tggctacatt
     4561 agtgaggggg ccggtagcaa ccaacgttat ttcgcctgga ttacgacaga tgagttcacc
     4621 tattgcttca ggcgcatggg gttctacctg ctggcacaat gctggagcag gaacattcga
     4681 ccagagttgc cgtaaaccaa actgatcgac accatgatcc agtttttcac gccagggttg
     4741 cggatcttcc aagagcgcac gcgaggcacc acggtagacc gcaacaggaa tatccaattg
     4801 tgtaatgaga tctctagcga ccgcatatcc cacgtctacc ggcgtatttc cggcaacagt
     4861 ggtgatcatt tccagcgcga tttgcggtgc ggcaatggcc agcgccagcg ccagaccgtc
     4921 gtcgatatta gcgccgggaa tgccgttccc cggatcgcag tcaataatga tacgcatcga
     4981 ttcattttca ctttttctcg ggttgaactt catgcccttg gcagccacac gactctcctg
     5041 ttcgtaaata aaactcaaat tcaacacggc gaacttctcc atcccaattt ttcagcatgt
     5101 caattgccgc acgggccatc ttatcgacag gttgtcgaac ggcggttaaa gaagggacgt
     5161 tataagccga ttcttgtgtt gcgttaaagc agaccagcgc aacatcttca ggaacgcgca
     5221 gcccatgttc tgccaaagcg cgtaaacagc cgagagcctg ttgttcattt gtcgcgaaca
     5281 gggcgcgtgg cagttggtgc tcaagcatgc gttttgtcgc ctcgtaaccg ccggcgcggg
     5341 tatagttggt cgaaaaaatc catgagggat tgaccactaa cgatgaggct tccagcgcct
     5401 gctgccagcc ccggatacga tcctgagtat tcagcatttc acgcgggccg cagatgatgc
     5461 cgatgtcgcg gtatccgtga tcaataagat gttgtgttac ctgcatcgcc gccagttgtt
     5521 catcaacctg gataacgctg acgttaagcc caggatccac acgatcgagc atgacgcagg
     5581 gggtaccgct ctcctgaatc aaattgatat aaggatggcg atcaacactg gtgtagatca
     5641 gcccgtcaac ctggcggtgt agcatattat taatgagttc acgttcacgg cagctactat
     5701 cgcctgcgtc gcccagcagg agtactttgc catcagcaaa ggcttcgtgt tgcagggcat
     5761 gagccataga ggcgataaac gggttcgaga tgtccggagc gactagccca taagtttgcg
     5821 tactccctga agccagcgca cgcgcgatgc cattgggtcg ataaccggtt ttcttaatgg
     5881 cctgtagtac acgttgtcgg gtcgcttcgg caacggggcg cgggccatta ttaataacgt
     5941 aactaacgac agcgacggat gtccctgctt cttttgccac gtcagaacgt gttacgcgtt
     6001 gtgaatgtga ttgagtcacg aagcacctac ctgattgcgt tcagagtgat tttttccaga
     6061 cagcataagg cgttatgacc caaaaatctt tggtaaagaa cgcgcatctc acgttctctg
     6121 gccttaaatt gcatcctcgg gtaagttgag atcccgtccg cgagtctctg gtgcaaagaa
     6181 agtggtcacc agtccaatcg cggccataaa ggcgaaatag atagcgattg gccaccagtg
     6241 cccgtaatac gagagtagcg cagaggcgat aagtggtgca gtcccgccag acagaatgga
     6301 accgagctct tttgcaaatg ccattttggt ataacgatta gtgacaccga aaagctcaac
     6361 gccccacgca gcctgaacac caaaaatacc cagtgaagcc agccccatcc cggtaatgat
     6421 cgtcgggata acaatccacg gctcacgaga atccagcaac ataaatgccg gaaaggcata
     6481 caggatcagt aacaagcaga accaacgata gatgatacgt ctaccgaatc tatcggacag
     6541 ccaacccgcc agaggaataa tggcaaatcc cagaacggat gcaataagta cagctgccgt
     6601 gggtacggac ttatccacca tcagtacttt cgcgacatag ccaatgataa atccctgagc
     6661 gagataagaa ggaccattct ctcctatgcg taatccgacc atggtccaga aggcacgggt
     6721 ccgtttccag aagctacgac tgtcatgttg ttgctgtgct ttttcctcac gaataacctt
     6781 ttctcgttca gcctgcagaa gggctttttg acgttcaaag actggtgttt cgcgtatatg
     6841 acgacgaatt aatagagctg cagcagcaat aagaatgctg caaagaaaag gaatacgcca
     6901 tccccagctt aataagtcat ccttgtccat ttgcaggacg atgagccaaa ccagcgaagc
     6961 cagtaatgtt ccgctgttcg aacccagacc aataacagat gaaaccagtc cacgtcgttt
     7021 aacgggagca tattcaccaa gcatcacagt accgcctgaa agttccgctc ctgctcccag
     7081 cccctgagaa aatcgaagga taaccagaca tatcggtgcc cagacgccta tctgggcgta
     7141 actggggatt aatcctatta gcatggtcga catgcccatc aatccaatag tgataaccat
     7201 gacaattttc cgaccatgtt tatcgcctat ccagccgaat agtaatgcac caatagggcg
     7261 ggcaataaac ccaacagaat aggcggcaaa gctggaaagt aatgccatga cgggtgtcgc
     7321 ctccggaaaa aagacatcgc caaagataat acctgcggca aggccgtaca gggcaaaatc
     7381 ggcatattcc atcgtggtgc ctaaccagca ggaaactgtt gctcgccaaa actcgcggcg
     7441 tccctcggag gtttccagcc gctcctgagc tgcctgttgg ttatctgtca gggaaacagt
     7501 ggatgaatgc gtgttcataa gaactccttg atattatccg tcagatatac atcacaggta
     7561 gatatctttc ttaaatctac tcatgtagat attgtgtttt attacatata aataaatcta
     7621 ctcgcgtaga taaaccattc aatggaagta aaatcgtgat agagatcact cataaggtgt
     7681 aagcgcaaag ctaacgttgt ctgttacctc gctcctagcg agctaaatta cgcttaaaat
     7741 atcagcaata cacgagcgcc ttgctcattt gagcagggtg ttttcttttg tcttacatac
     7801 agtcatgacc atgaaattag tcaactactc gggcagcgcg tttatgggcc gtgagtaacg
     7861 aagttggatg caaatgtcag atcatgtgcg cctgttaggg cgcggctggt aagagagcct
     7921 tataggcgca tttgaaaaac ctccggctat gccggaggat atttattttg ctaccacagt
     7981 gacagtcact gctggaaaat atacctctgt tcaaaacagt cctgttgtgg atccgaaaaa
     8041 tacacatcgc tcccgttatc tgtggctgta tgattaccat cactgagtaa aaaaaatccc
     8101 caggatgcag gggacggaag gtaaactatg ctgaatcttg tctgtttgtt ttaatattta
     8161 tctatccgtc ttgccaacgg acaaagaaaa aataacacga cgcagaacaa aagaaaatat
     8221 tattttcata tcttccggaa gactatggcc tcttccagat aattacctta tggttggcat
     8281 caatcaatga ttcactctcg ttctgcctcg gaactctata tggatgtaca atcatataat
     8341 gttctaatat tcacgagatg tgaataaaca aatccactta agaaacgggg gggtatttaa
     8401 ttaataacta agtgaagcgc cccggaaatc ttggagacta aattccctga gaaagaggta
     8461 aacaggatgg ctaaaaatac tcgttttccc ccgaagtccg tcaacggaca gttcgtatgg
     8521 ttctggaaaa tcagggcgaa tatgactccc aatgggcagc aatttgttcc atcgtcccaa
     8581 agattggctg tacaccggag actctgcgtg tctggtgtac aaccagcatg agtgggatac
     8641 cgggggcggt gatggtggac tcaccaccgc tgaacgtcag cgtctgaaag acgctggagc
     8701 gtgaaaatcg tgaactgcgc cacagtaacg atatccttcg ccaagctctt attttgcgaa
     8761 ggcggagttc aaccacccct ggaaactgtt gccagttcat tttcgccagg cagcacgcta
     8821 cctgctggca gttcgccgca caggatgcgc tcggcaagat gctgagtaac agattcggag
     8881 agactagtct gcacaatttt cattgcattt cctagctaaa aaacgtcaca gtgatgagcc
     8941 atataataag ctaaataaca gcaaacttac taattgtgaa gcaggtgaac tccaccccct
     9001 agtcagttct tatcgttgct ggataccctc aaagcagtgt aatgctatcc agaataggca
     9061 tgcttaagga agagctcaag ggtagaaccc cgacttcggc atcgcagagg atactgtagt
     9121 ggtcaagtaa tactggccac ggttttacag taaaaatg
//
LOCUS       DARNVM010000181         8921 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.181, whole genome shotgun sequence.
ACCESSION   DARNVM010000181 DARNVM010000000
VERSION     DARNVM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.181"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..182)
                     /gene="ralR"
                     /locus_tag="SI621_004076"
     CDS             complement(<1..182)
                     /gene="ralR"
                     /locus_tag="SI621_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HEI4066725.1"
                     /translation="MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGG"
     gene            complement(246..1334)
                     /locus_tag="SI621_004077"
     CDS             complement(246..1334)
                     /locus_tag="SI621_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEI4066726.1"
                     /translation="MQNTNIITTEQTPNTISASNTIFNVQALTQLQAVAGLMAQATVT
                     VPDHLRGNPADCMAIIMQAMQWGMNPYAVAQKTHLVNGVLGYEAQLVNAVISSSSAIV
                     GRFHYKYEGDWEKCSRTRVEIVKKTAKGGGIYEKKETIPCWTSEDEYGLSVRVGAVLR
                     GESEITWGEPVFLSSVITRNSPLWTSNPKQQLAYLALKYWARLYCPDVILGVYTPDEL
                     EEPQEKIINPVSVQNYSEVSEQRTETIEQRIDEAWIDEFRQRIESAATTEETTALRQE
                     IEDQKNQIGEFFAELKGKVVRRHHRLNAIASIEKMINDLPSSGDPEAEQKFTALENTL
                     NAARPHLGELYEAYKTTLTDMKPEYIGS"
     gene            complement(1349..4498)
                     /gene="recE"
                     /locus_tag="SI621_004078"
     CDS             complement(1349..4498)
                     /gene="recE"
                     /locus_tag="SI621_004078"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HEI4066727.1"
                     /translation="MSTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     SSAQDKPDSTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QTLHIARAEELPAVTALAVSHKTSLLDPLEIRDLHRLVRDTDKVFPNPGNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKYQKLRAE
                     LHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIETLDSVNQNQQETEQ
                     NDKKAEQNSPNTQQNEPETKQPEPEEQQEPEKVCTACGQIGGGNCPDCGAAMGDATYQ
                     ETFNEENLDESLEKDPEEMEGAEHQHNENADSDPHRNCSDETGEASAPVATEIMWPSY
                     FEPGRYENLPNEVYHSANGISSTMLKDARISLMYYHGRHIAGTIPNEESDALLRGRII
                     HSYVLETDKFADEYAIPVPVPEYVVTTSNELIAIIKKHNASLPALMTPEQMKEWIESY
                     NSTLIQPLSVSAGAEETGILYGSLPVEFRRIPEGEKHTASAMKACIKEYNASLPPLLK
                     TSGAREQLLDQIETVDPELAKKERAKSLPYNISGTKEQLTEIARKIRPELVTLEDWQK
                     RQQEENAGKTFISPDMYEQAKNIHAALQNNTDAARLLNHPDRKSEISYFGFDEETGLE
                     IRVRPDIEIRLPYESICADVKSVSLGYVRQERLKDRLHREIIERDYHLSAAMYCDVAN
                     LDKFFWIFVNKDAGYHWVAVVEASQELLELGRQEYRRTLRQINEALETNNWPAPITES
                     YTDELNDFDLRRLEALHLA"
     gene            complement(4598..4873)
                     /locus_tag="SI621_004079"
     CDS             complement(4598..4873)
                     /locus_tag="SI621_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066728.1"
                     /translation="MITSYEATVVTTDDIVHEVSLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            complement(4948..5124)
                     /locus_tag="SI621_004080"
     CDS             complement(4948..5124)
                     /locus_tag="SI621_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066729.1"
                     /translation="MVMAKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            complement(5118..5339)
                     /gene="kilR"
                     /locus_tag="SI621_004081"
     CDS             complement(5118..5339)
                     /gene="kilR"
                     /locus_tag="SI621_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HEI4066730.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            5735..5929
                     /locus_tag="SI621_004082"
     CDS             5735..5929
                     /locus_tag="SI621_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066731.1"
                     /translation="MDNKDKAWLLALAFSIRSQRETTHQEFFSEIENAENEFLSLLNE
                     RDAKKSADSLKAWAKLGSSK"
     gene            complement(5895..6113)
                     /locus_tag="SI621_004083"
     CDS             complement(5895..6113)
                     /locus_tag="SI621_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066732.1"
                     /translation="MVGEDLVVINGKLCSKDVAAMLISKVLPTVLEVIAEKVKAGRPD
                     KEVEEAAKTVVHAATEAIILKSLVSPTP"
     gene            complement(6362..6823)
                     /locus_tag="SI621_004084"
     CDS             complement(6362..6823)
                     /locus_tag="SI621_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066733.1"
                     /translation="MKKTREVIATPEASKNLKAAWNARKKELKLTQELAAELLGFESQ
                     GTVSQYLNGKIPVNTDAALKFAVLLKVKPEDIREDLKDLMNYVRSSDTYDDSFSGNGW
                     RLVNEEQAELLNLFEILPASEKAKLLNQLRGLNKLYEEAFENMLALKKRNQ"
     gene            6931..7206
                     /locus_tag="SI621_004085"
     CDS             6931..7206
                     /locus_tag="SI621_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066734.1"
                     /translation="MNLRDYLKEKHITQLQFGKLTGLSQVHVSRVLGGYERFSPEKAL
                     RVAEVTNFEVTPHELRPDIYPNPTDGLPVGCKANTQNAQELIHENQA"
     gene            7190..7612
                     /locus_tag="SI621_004086"
     CDS             7190..7612
                     /locus_tag="SI621_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066735.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQTLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            7690..8478
                     /locus_tag="SI621_004087"
     CDS             7690..8478
                     /locus_tag="SI621_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066736.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDVGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAAGVTQTDQKALPEEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            8485..>8921
                     /locus_tag="SI621_004088"
     CDS             8485..>8921
                     /locus_tag="SI621_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEI4066737.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHA"
BASE COUNT         2127 a         2084 c         2124 g         2586 t
ORIGIN      
        1 cctccattat tatttccagt ggttcgttta ttccatcgtt cgagtgcttc tttttcactt
       61 ccaccatagc cggttcggga ttcgcatccg ttacacttcg cgcggtaata tcctgaaatg
      121 gctttcaccg ttactgatgg acaaccacaa aaagggcatg gtttgacttt ttcataccgc
      181 attgtctttt ctctcatata ataaagtttt gtgatggcgg tgaggctaca ccgccaaagt
      241 caatatcagg agccgatata ttctggtttc atatctgtca gtgtcgtttt atacgcctca
      301 tataattcac ccagatgtgg tcgtgcagca ttcagcgtat tttccagagc agtaaatttt
      361 tgttctgctt ctggatcacc tgatgaaggc aggtcattta tcatcttctc gatactggca
      421 atagcattga gacggtgatg acgccgaacc acttttcctt taagctcggc aaagaattcg
      481 ccgatctggt ttttctgatc ctctatctct tggcgtaatg cagtggtttc ctcagtcgtg
      541 gccgcgcttt ctatacgctg ccggaattca tcaatccacg cttcgtcaat acgctgttcg
      601 atggtttctg ttcgctgctc gcttacctcg ctataattct gtaccgacac aggattgatg
      661 attttttcct gtggctcttc cagttcgtcc ggggtataca cgcccaggat gacgtcagga
      721 caataaaggc gagcccagta tttcaacgcc agataggcga gctgttgttt cgggtttgag
      781 gtccataaag gagaattacg cgtaatcacg ctggaaagaa acaccggttc tccccaggta
      841 atctcacttt caccgcgaag aactgcacca actcgaactg agagaccata ctcatcttca
      901 ctggtccaac atgggatcgt ttcttttttc tcatagattc cgcctccttt ggccgttttc
      961 ttaacgatct ccactcgggt gcgagagcat ttctcccagt ctccctcgta cttgtaatgg
     1021 aagcgaccta caattgcact tgagctagag atcacagcgt taaccagttg tgcttcataa
     1081 cccagaactc cgtttaccag gtgtgttttc tgagccacag cgtaggggtt catgccccat
     1141 tgcatagctt gcatgatgat tgccatgcag tcggcaggat ttccgcgaag gtgatcggga
     1201 actgtgacgg tggcctgtgc catcaacccg gctactgcct gaagttgcgt caacgcctga
     1261 acgttaaaaa tagtgttact ggcagaaatg gtatttggtg tctgctctgt cgtgatgata
     1321 ttggtatttt gcatggtcag gttctccatt aagccagatg cagtgcttca agacgacgaa
     1381 gatcaaagtc gtttaattcg tcggtataac tttcggtaat cggtgctggc cagttgtttg
     1441 tctctagagc ttcgtttatc tggcgtagcg tccggcgata ttcctgtcga ccaagttcaa
     1501 ggagttcctg cgaggcttcc acgactgcca cccagtgata gccagcatct ttgttgacga
     1561 agatccagaa aaatttgtcc aggtttgcca catcgcaata cattgcggcg ctgaggtgat
     1621 aatcacgctc aataatttca cggtgcaggc gatctttaag tcgttcctgc cgcacataac
     1681 cgaggctgac tgacttcacg tcagcgcaaa tgctttcgta tggcagccgg atttcgatat
     1741 caggacggac cctgatttcc agcccggttt cttcatcaaa cccgaaatag ctgatttcag
     1801 atttgcgatc cgggtggttg agtagccttg ctgcatcggt attgttttgc agtgccgcgt
     1861 gaatattttt tgcctgttca tacatatccg gactgataaa cgttttcccg gcgttttctt
     1921 cttgctggcg tttttgccag tcctccagtg tcaccagttc cgggcgaatt ttccgtgcga
     1981 tttcggttaa ttgctctttt gtgccactga tgttgtaagg caaagattta gcacgttctt
     2041 tttttgccag ttctggatct acagtttcaa tttgatccag aagctgctcc cgtgctccac
     2101 tggttttcaa cagaggaggg aggcttgcgt tgtattcttt aatacaggct ttcattgctg
     2161 atgctgtgtg tttttccccc tcaggaatac gccgaaattc caccggaagc gaaccgtaaa
     2221 ggatgcctgt ttcttcggcc ccagcactta cagacagtgg ctgtataaga gtgctgttgt
     2281 agctttcgat ccactctttc atctgctctg gtgtcatcag tgctggcaga ctggcattgt
     2341 gttttttaat gatggcgatc agttcgttag aagtagtaac cacatattca ggaaccggta
     2401 ccggaatggc atattcatct gcgaatttat ccgtttccag aacatagctg tgaatgatcc
     2461 gcccacgcag cagtgcgtca ctttcctcgt tcggaatagt tccggcaatg tgccgcccgt
     2521 ggtaatacat caggctgata cgggcatcct tcagcatcgt gctgcttatt ccgttggcgg
     2581 agtgataaac ctcgttcggg aggttttcat agcggcctgg ctcgaaatat gacggccaca
     2641 tgatttcagt tgctacagga gctgacgctt cgccagtttc atcactgcaa ttgcgatgcg
     2701 gatcgctgtc agcattctca ttgtgctgat gttcagcgcc ttccatttcc tccggatctt
     2761 tttccagaga ttcatccaga ttttcttcat taaatgtttc ctgatacgtt gcgtcgccca
     2821 tcgccgcgcc acaatcaggg cagttgcccc caccgatctg accgcaggcg gtgcagactt
     2881 tttccggttc ttgttgctct tctggttcag gctgtttcgt ttctggctcg ttttgttgcg
     2941 tatttgggct gttttgttcc gctttcttgt cgttctgttc cgtttcttgc tggttctggt
     3001 tcacagagtc gagggtttca atccccttaa cccatttcgg atcgttcggg tcgctaatcc
     3061 cttcaacaaa ttctccgcga gaggcagcca gtaatttgtc tgcatcgaca ggatttttgg
     3121 gcggaatgtt tttccgggct tcatggagtt ctgcccgcag tttctgatat ttcgcatcaa
     3181 cagaatttac ctgtgactga gcatccagcg gctgcgtgtt ctgatgatgt tcagttgcat
     3241 tcggttccac tgtttcagcc gttgcctgtt catctgccat tgcgccagat ggttgtggtt
     3301 tttcttcatc gtcctgtttt ccttcttctg ttactcgctg cggcatcggg gcagaggagc
     3361 gaccgcaggc aatatccacg atttccggat cagggttggc atgatcggtt tcagtcagta
     3421 ctttgttcag atattcagtg acgtgtgcgg ggatgacctc gatcccaatt ggtgcttctt
     3481 tcacggacgc aaccacgatg gcgcgggaat aatccagccc gccaggcatg gtgatgaatt
     3541 tgtcgcggaa aacagaaaag ggtggtttat tttcagcgat aatttcctca atgcgtttag
     3601 cgtgtgccgg atgaaggtta taaatgtcca cgtccattga acgggccagt acgccagtgg
     3661 ctacatcgcg tgccagtgac gtcagatcgt ggacgaaacc ttcgccgcga tcggtgaggt
     3721 tcccgccgcc agcattagca ccggaagccg tgcgagtgat gcgtgaaaca cgatttcctt
     3781 tcatccactc ttttgtcagc agaccgcgat cggtgtagtc tgcgtccagg tatgcttcga
     3841 aaaaagcagt catcagcccc agacttgaat tgcctggatt agggaaaact ttgtcagtat
     3901 cacgaaccag tctgtgaaga tcgcgaatct ccagcgggtc gagcaggctg gttttgtggg
     3961 aaacagctag ggcagtaaca gctggtagtt cttcggcccg tgcaatatgt aatgtctgga
     4021 gttcgtcgcg tgaaacgtgc gttaccggtt tttcgctgcc gtgttgagca agccagcgaa
     4081 tgggcagctc ttggccagaa atcgggagta gcatattctc ctcaatctca gtcatgtctt
     4141 cgccattaac attggtattg tcagtgctgt ctggtttgtc ctgcgcagag gatgagggcg
     4201 cgataaatac cattgtgatg ccatcttccc cgcctttttc gtaacggttg cagaattccg
     4261 tatcaaatac gccttctggt ggaaggtcat caacaacggg caaattgacg cgaacaggtt
     4321 ttttaaagtc atcttcatcg tagccagcat cgtcaatcgc aacagcacca cgggagatgg
     4381 caatggataa ttttttcgct tcagcccagt agaaaccgcc tttaataccg agacgttttc
     4441 ttactttgtc attttttgct tcgtaataca gtgggtaaac ttgtttatcg gtgctcattg
     4501 ttttttaacc tcaactcaga ttaaaattac tgcgagtgat gaataaatgt ccatggttct
     4561 tcactcaggc ctgcacagtg tgcaggcttt ctttttttca gatttcacct tttaatttca
     4621 ttgcaatcag agttgccaga aattcggctt ttttttctgc gggcagattc tttcctatgt
     4681 gcaccagaca cattttttta acaccatcgt taagtgtttt aacgttgcct gatggaccgt
     4741 cgatatcaac cacagtgaat ggggtttctt tattttctgt cttaatcacg tagccaatac
     4801 gctttccttc caggctgact tcgtgaacaa tgtcatcagt agtaacaaca gtggcttcat
     4861 aactggtaat catgtttttc tccttaatta aggttgagcg aatccctgcc attgctggca
     4921 taaattcagt ttcggatagt cagttaatta aagttcgtgt gccatctggt ctttttcggc
     4981 acagatttca ctacaatatt ttttcatttc cgtcgttggt ataactccac gcatgaaatg
     5041 aagtggtctt gtaatgattt tgctttcttc aatttcttta ttgcaaaggt gataagcaca
     5101 ttttattttc ttagccatta ccatgactcc gcctttacag gtaaaccatc acgaccgagg
     5161 aagactttaa tcatgcagtc agaaatgcat gtttttgtag tcaggctacg aatataaagt
     5221 tttcgctttt taatattgtt tgccgaggcg atatatgtcc gaccttcatg aagaacataa
     5281 tcgccagggg tcacacactg acgtggtatt tcatcagttc cgaagtgatg agcaatcata
     5341 attatctcca tttttacaaa tgaattttgt cgatgcggtg cctggtgcct ccaggtgacg
     5401 ttaaccagtt aacaattaac gccggataat ccacccataa cactgatgct tttaactgtg
     5461 ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag gggcggcagg
     5521 gcagccacgg agtagaactg atgccgccaa agactagaca cagcaatgtc gttatttaca
     5581 accggaggcg cactcccacc atttaaattt aacagacaag accgactctt tatgaatacc
     5641 ggaaatgcgc cttcgtgttg tgcgcctgtc tttttaccac ttcaggctcg gtggtatact
     5701 ggagttctca cacaaccagt aataaggtat tcctatggat aataaagaca aagcctggct
     5761 acttgcttta gctttcagca tcagatcgca gcgagaaaca actcatcaag aatttttctc
     5821 ggaaatcgag aatgctgaga atgagttttt gtcactactc aatgaacgtg atgctaaaaa
     5881 aagcgccgat tcacttaagg cgtgggcgaa actaggctct tcaaaataat tgcttctgta
     5941 gcggcatgaa caactgtttt tgccgcctct tcaacttcct tgtctggacg accagccttt
     6001 actttttctg caatgacctc cagcacagtt ggaagaacct tgctaataag catggctgcc
     6061 acatctttgc tgcacaattt accgttaata acaaccagat cttcaccaac catctttttt
     6121 ccccttaacg ccgggtggcg gaactaaaac ctacagcgcc gtgctgttct tgatggaaat
     6181 attagtaacg cggatatttt aagtcaacag tatggcgtat gatatttttg atttggtaac
     6241 tatgtaaatg ttttttcaag ggaaaaatat tagttataca gctgatttgc agaagttatg
     6301 gcacaaaaaa aaccgactaa gacgtcggtt tttttgttgt ggatggggta gtgagcggtg
     6361 gctactggtt acgtttcttt agtgccagca tgttctcgaa ggcttcctcg tagagcttgt
     6421 ttagtccacg tagctggtta aggagtttgg ctttttctga cgcaggtaga atctcgaaga
     6481 ggttaagtaa ctctgcctgt tcttcattga ccagcctcca tccgttgcct gaaaaactat
     6541 catcataagt atctgatgat cttacataat tcattaagtc tttaaggtct tctcgaatgt
     6601 cctctggttt tacctttaac agaaccgcaa attttagcgc agcgtcggta tttaccggta
     6661 tcttgccgtt cagatactgg ctaacggtgc cttgagattc gaatcccaac aactcagccg
     6721 ccagctcttg agtcagcttc agctcttttt ttcttgcatt ccatgcggct tttaaattct
     6781 tgctcgcttc tggagttgca atcacttcgc gtgttttttt catacataga gtttatttgt
     6841 tttaccaata ttatcaaaga tagtctgact attgatcttt aaaattagcg gagctaatat
     6901 ttgctcgagg cataacgtag aaggttggct atgaacttaa gagactattt aaaagagaaa
     6961 catatcaccc agctacagtt tgggaagcta acgggtttat ctcaggtgca tgtaagtcga
     7021 gtgctggggg gctatgaaag attcagccct gaaaaagcat tacgtgttgc tgaagtaacg
     7081 aatttcgagg ttacacctca tgaactccgg cctgatatct acccgaatcc gaccgatggt
     7141 ttacctgttg gatgtaaggc taacacacaa aatgcacagg agttgattca tgaaaatcag
     7201 gcatgagcac atcgaatcag tgctgttagc cctggccgct gaaaaagggc aggcatgggt
     7261 agccaatgca attactgaag aatatctgcg ccaggggggc ggcgaattgc ccttggtacc
     7321 aggcaaggac tggaataatc agcagaacat ctatcaccgt tggttaaaag gtgaaacgaa
     7381 agcgcaaagg gaaaaaattc agacactgat ccctgcggtt ctggcaattc ttccgcgcga
     7441 gctgcgtcac cgactctgca tcttcgatac cctggaacgc cgtgcattac tggcggcgca
     7501 ggaagcgttg agtacggcaa ttgatgcgca tgatgatgca gtccaggccg tttaccggaa
     7561 agcacatttc agcggtggtg ggtcgcccgg tgattctgtc gtagtgcatt gattgaaatt
     7621 aatcgtgccg gattgttttg ttcggtatca gttaaatgta acgctgcgag cgttacaagg
     7681 tgaaaacaaa tggcttcaaa ctggataaag ctcgaggtta ttacgccgga taagccggaa
     7741 atattcaggc ttgctgagat tctgaatatt gatccagatg ccgcattagg gaaggttatt
     7801 cgcttctggg catgggcgga tcaacaaatg atagacggta atgcagattg taacgctcgc
     7861 ggcgttacaa aaagtgcaat agatcgcatc acttttatgg ctggttttgc tgatgcgtta
     7921 attcaggttg gatggctggt cgaaaatgac gttgggcttt ctctacctaa ctttgaacgc
     7981 cataacggaa aaagctctaa aaaacgggcg gttacaaacg agcgagttac aaaaatacgt
     8041 gaactgaaac gaaaaggtaa cgctgccggc gttacacaaa cggatcaaaa agcgttacca
     8101 gaggaagagg aagaggaaga tctaaatact gatctccccc taaatccccc tcgccaaaaa
     8161 cgagcgtcta aaaaattcga gccggaggct attgagctgc ccgattggtt gccggaaaca
     8221 ctctggcatg agtgggtccg gttcagacag gcattgcgaa aaccgattcg aacggagcag
     8281 ggcgctaacg gggcgatacg ggaactggaa aaattccgtc agcagggttt tacacctgag
     8341 caggtgattc gacacagcat cgccaatgaa taccagggcc tgttcgcgcc gaaaggtgtt
     8401 cggcctgaga cgttgctccg acaggttaac accgtctcct tgccggacag tgcgatcccg
     8461 ccaggcttca gggggtaaca gaccatgaaa aatattgcga caggcggcgt tctggagcgt
     8521 atccgcagac tggccccacc acatgtaacc gccccattca gaacggttgc ggagtggcgc
     8581 gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga tcaaccgcct gaatcgtcag
     8641 ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc cgttgcaccg taaatgctcg
     8701 tttgcgaatt accaggtgca gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc
     8761 atcgccgatg aactgatgac cgggtgtaca aattttgcgt tcagcggaaa acctggtacc
     8821 gggaagaatc acttagcggc agctatcggg aatcgcctgc tgaaagacgg tcagacagtg
     8881 attgtggtta ccgtggctga tgtcatgagt gctctacacg c
//
LOCUS       DARNVM010000182         8896 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.182, whole genome shotgun sequence.
ACCESSION   DARNVM010000182 DARNVM010000000
VERSION     DARNVM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.182"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..124
                     /locus_tag="SI621_004089"
     CDS             <1..124
                     /locus_tag="SI621_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEI4066738.1"
                     /translation="VEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEHQKAG"
     gene            complement(224..1084)
                     /locus_tag="SI621_004090"
     CDS             complement(224..1084)
                     /locus_tag="SI621_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEI4066739.1"
                     /translation="MNIAIVTINQENAATASWQAAQDFSGCTLAHWKIEPQPAVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLVSIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHAECQ"
     gene            complement(1081..1860)
                     /locus_tag="SI621_004091"
     CDS             complement(1081..1860)
                     /locus_tag="SI621_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEI4066740.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAVGKAAIIRKTVAAESPA
                     MQCLQLARAEQQRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(1838..3247)
                     /locus_tag="SI621_004092"
     CDS             complement(1838..3247)
                     /locus_tag="SI621_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066741.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGIKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(3269..4723)
                     /locus_tag="SI621_004093"
     CDS             complement(3269..4723)
                     /locus_tag="SI621_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEI4066742.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(4793..5578)
                     /locus_tag="SI621_004094"
     CDS             complement(4793..5578)
                     /locus_tag="SI621_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEI4066743.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILINNA
                     GICKLNKVLNFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            5898..7175
                     /locus_tag="SI621_004095"
     CDS             5898..7175
                     /locus_tag="SI621_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066744.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPIAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            7202..8680
                     /locus_tag="SI621_004096"
     CDS             7202..8680
                     /locus_tag="SI621_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEI4066745.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKLLPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKELTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         2233 a         2296 c         2176 g         2191 t
ORIGIN      
        1 tgtagaagag ctggaaaatg ccgttaacga atacataact tactggaatc aaaaaagaat
       61 aaaactcagt cttgggggac tcagtccggt agaataccgg actgagcatc aaaaagccgg
      121 ttaactgaaa ctgtccaggt tttggggttc agttcattga cgcgtcttat caggcttaca
      181 agtccccagc ccgaaaccgt aggcaggatc atccggctgc acgttattgg cattctgcgt
      241 gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg
      301 cctgtgaaaa taccgctgcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc
      361 gtacgcccgc cattaacgct gctgcgccgg aagcgccaac aacaatacac acctcaggtg
      421 ccagcagatg tccagaaata ccaatcactt tttcggcatc aacgccaccg ttcatcactc
      481 gtgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg atttcctggt
      541 tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct aatgggtcgc
      601 gggtaacatt tttcaggttt tctatgctaa ccagccagtc gggaagcgca cctgggacaa
      661 tgttcagttg ctgcatacca gagggcaacg tggcgttttt atctgctccc gcctgacgtg
      721 ccagcgaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc
      781 agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg
      841 caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa
      901 agagaacaac gtccgctggc gttcgctgcc actgttccac cagcgcatcc agcacctgtt
      961 ccgccacagc gggctgaggt tcaatcttcc agtgcgccag ggtacaaccg ctgaaatcct
     1021 gcgccgcctg ccagctcgct gttgcggcat tctcctgatt tattgtcaca attgcgatat
     1081 tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgcgctt tttctgctac
     1141 cgtttgccca tcaatcagcg tcgctccgcg ttgttgctct gctcttgcca gttgcagaca
     1201 ctgcatcgct ggcgattcgg cagcaacagt tttacggata attgctgctt tccccaccgc
     1261 cattcgctga cgcattcccg gtactggcaa ggccacttca ccgcactgac gcacggcaat
     1321 caccgcaggc aagcgaaccc ggcaacagcg cagcccgttt tcagtacgct gttcaagggt
     1381 gataaacggc gcgtcgagag taaaacgctc cacctgggtg aagcagggcc agcccagcat
     1441 ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccagtaat
     1501 tatcagctcc agcggattct ggtactgcca ttcggcaata tggcgagcga caaattccgg
     1561 cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat
     1621 aagatagcgc agccagtgca acgcccgttc atcccccatg ctcaacgcgg ttaaagacat
     1681 cgacgtaccg ttttttctct gcgccaacag cagcgcggcg gcagcctgtt catcagcacc
     1741 gagcaaactt cgcagaagcg aaacatccgg tccgctatta ccctgagccg ccgcctgcca
     1801 ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat
     1861 tcgcacctcc aacatttccc gccgccacca gcgggagtgc tttggtttct ggtgcccata
     1921 gccaggtcac gacaaaacca accaacaaca ctgccgccag gagcaataag gtgacttgca
     1981 ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg cccaaccgac
     2041 tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag
     2101 gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca
     2161 gcagcgttaa tgaactgccg gaaggcaaac aagccatcac taccagcgtt gccgccagca
     2221 gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca
     2281 gcagcgcacc cacaattaac aacgcattaa gcatcaggct agcggtcaac gcatcttcca
     2341 gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga
     2401 ggcagacaaa gaagacgctg ttaaacgctg tgcgccgcca gtagcgcgaa gagaacaagg
     2461 ttttgatgtg tttatgggtc gccgttgcca cttcatcgcc cagtaaaaca tggggaccaa
     2521 aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg
     2581 gcgattctgg cgttccccag cgtaataacg taatcaacaa tgcgggcaga gctgccgatg
     2641 ccagcagcca gcgccaggcc tccgggcttt cgctaataaa gtgatgtccg gcaatacttg
     2701 ccagcacata gcctacagtc cacaccacgc tgaacgcgcc cagcaaaata ccgcggtggc
     2761 ggcgcgggga aaattcagcc agcaaggtgt gaccgactga gtaatcgcct cccagaccaa
     2821 tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgtaaaa
     2881 acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgtcgc ccaatatggt
     2941 cggagatcca gcccagaacc aggctgccaa gaaacaaacc aaggagtgct gagccgccaa
     3001 tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac
     3061 caatgacgcc gaggacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaatgc
     3121 ggcagtgaaa acggttaagt ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta
     3181 tcccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc
     3241 ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat
     3301 tcataatgcc gttaggatcg aactgctttt tcagaccttc cagcaacgtc cacgcgctgc
     3361 cgtgttccag cttgctccag tgaacgcgat gtttaccaat gccgtggtgg tgcaccatcg
     3421 aaccaccgag gcgaatggtt tcttcgcaga tgattttgtt gagcggattg tggtacttgt
     3481 cgatttcctc ttccggctta cagtcaacaa cgttgtagtc gtaaacgaag tacatgttgg
     3541 tgccgttctg gtagctgtga gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg
     3601 ggaactcggt acgaatacgg ttaataacgc tttcgtagat ttcgtgaatg cagctccagc
     3661 agccggacac ttcggtggta aagcccatgt tgccggtttt gaggatctgc acacgttcgg
     3721 cagctacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc agcttgctgt
     3781 ccactcgctg gcattgcggg taacgtgcca cgatttcagc aatcccttcg cccgtcgcct
     3841 tcgcaatgcg agggttacct tcagccataa agatcagcac gcattttccg tcagcaaaat
     3901 gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc
     3961 cttccaccat gatttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc
     4021 cgtagaagag gttgttttcc ggggtgaact taaagatttt cacagttact tcagtgatat
     4081 agcacaatgc accttcgttg ccgatgatga tgtgacgaat gtccgggcct gccgcgcggc
     4141 gtggtacgtt tttaatgcgt gttacggtgc catcagccaa tactgcttcc agaccaacga
     4201 ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca
     4261 ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt
     4321 cacgcaacgc gttttccagc acttccagcg gaactccgca ttgcgccgtg gcctgcatat
     4381 tctcaatatc aatattaatg atttgattca tggcagagcc gtcgagcacc accgagtttt
     4441 ccacaacggt ttccagccca ccttcggtag cggaagcacc ggtacgcggc acaccgttaa
     4501 ttttgtgcgc attcataaaa ttcagcacac gggatacttg ttctgtggaa ccgagtttta
     4561 cgaccgctgc cggaatcggc aaagtataaa tgccatgaat atccggaaat ttgcgaaaac
     4621 ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa
     4681 tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat
     4741 aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg gattagcgca
     4801 ctaaataacc accatcaacc actaataaat gtccgttgac ataattcgat gccggacttg
     4861 cgaggaatac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat
     4921 gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta atatctgttg
     4981 cataatagcc aggggcaata ccatttacct gaatattata ttgacctagt tcatcacagt
     5041 aagctttggt gaatccggca agagcatgtt tagtggcaga atatgcaggt gaccattgtc
     5101 cacctaagta agagaataat gaacagatat taatgatttt accgcttttc tgcgggatca
     5161 taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg
     5221 ggtcccagtc ggcacgaccg aagttcagca ccttattcag cttacaaata ccggcattgt
     5281 taatcagaat atcgacggta ccgaaacgtt cgcaacaggc ggcgattatc ttctgcggcg
     5341 cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt ttttcaatca
     5401 tttcctttgt ttcgccgtta tctttaacga agcttggaat aaagatattt gcgcctgctt
     5461 tggccagcgc catggcaaat gcctggccta aaccgctatt cccgccggta acaattgcgg
     5521 ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag
     5581 ttagctccaa tttccgtaag gcaaaaaaaa aagaaaggta acttcccccg cgagagggaa
     5641 gttacctttc tcattatctc tggtaactgg agataatttt tatcataagg gcataatcgc
     5701 taatgtgatc gtaatcacag tgtgatattc gaattgtcaa ataattttaa aaagcagaag
     5761 cgtgattact ctcacacaac accactgctt tccatgttat ttcttattgc aattactaga
     5821 gacctgagaa aagtaatcca cctgaaaggg cggcttactt ttctcttttt tttgtctatt
     5881 acaaatggga atacgatatg caacacaact catatcgccg ttggataacc ctcgcgataa
     5941 ttagttttag cggcggcgtt agtttcgacc tggcttattt acgttatatt tatcaaattc
     6001 ccatagcgaa atttatggga ttcagcaata ccgagatagg tttaataatg agtacatttg
     6061 gtattgcggc cattattctt tatgccccca gcggcgttat tgccgataaa ttttcacacc
     6121 gcaaaatgat tacttccgcg atgatcatta ccggattact gggtctgtta atggcaacat
     6181 atccaccgct gtgggtaatg ctctgtattc aggtcgcctt tgcgataacc actattttaa
     6241 tgctgtggtc ggtgtcgatt aaagccgcct cattgctggg cgatcatagc gagcaaggga
     6301 aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg gcggtgttta
     6361 ccatgtgggt cttttctcgc ttcgctccgg atgacagcgc cagtctgaaa acggtcatca
     6421 tcatctatag tgtggtttac atcttgttgg ggattctgtg ctggtttttt gttagcgata
     6481 acaacaatct gcgcagtgcc aataacgaag aaaaacagtc attccagctt agcgacatcc
     6541 tggccgtttt gcgcatcagc accacctggt attgcagcat ggtgattttt ggcgtcttca
     6601 ccatctacgc cattctgagt tactccacca actatctgac cgaaatgtac ggcatgtcgc
     6661 tggtggcggc gagctacatg gggattgtga tcaacaaaat cttccgcgcg ctgtgcggcc
     6721 cacttggcgg cattatcacc acctacagca aagtgaaatc ccctacccgc gtgatccaaa
     6781 tcctttccgt actcggcttg ctggcgttaa ctgccctgct cgtcacgaac tctaacccgc
     6841 aatcggtcgc gatggggatt ggcctgattt tactgctggg attcacctgt tacgcctcac
     6901 gcgggctgta ctgggcctgc cctggcgaag cgagaacacc gtcttacatt atgggcacca
     6961 cggtaggtat ttgttcggtg attggattcc tgccggatgt cttcgtttac ccgattatcg
     7021 gctactggca agacaccctg cccgctgcag aagcctaccg caatatgtgg ctgatgggca
     7081 tggcggcgct tgccatggtg atcgtcttta cctttttgct gttccaaaaa attcgtactg
     7141 ctgatagcgc ccctgctatg gctagcagca agtaagtcca gaaaattaca ggagaaaaat
     7201 catgtcgaaa aaatacatca tagggattga tggtggaagt cagagcacaa aagtggtgat
     7261 gtacgatctg gaaggtaatg tggtttgcga aggcaaaggc ttattgcagc cgatgcacac
     7321 gccagatgcc gataccgcag aacatcctga cgacgattta tgggcatcat tatgctttgc
     7381 cggtcacgat ttgatgagtc agtttgccgg aaataaagaa gatattgtcg gtattggtct
     7441 gggatccatc cgttgctgcc gtgcgttatt gaaagccgat ggcacgccag ctgcgccgtt
     7501 gattagctgg caggatgcac gcgttacacg cccttacgaa catacgaatc ctgacgtggc
     7561 atatgtcacc tctttttcgg gttatctgac gcatcgctta accggcgagt ttaaagacaa
     7621 tatcgccaac tattttggtc agtggccggt ggattataag acctgggcat ggagtgaaga
     7681 tgccgcagtc atggagaagt ttaatatccc ccgtcatatg ctgtttgatg tgcaaatgcc
     7741 gggcactgtt cttgggcata tcactcctca agccgcgctg gcgacacatt tcccggctgg
     7801 gctgccggtt gtttgtacca ccagtgataa accggtagaa gcactgggag ccgggttact
     7861 ggatgatgaa actgcggtaa tttctcttgg cacttacatc gcgttgatga tgaacggcaa
     7921 attactaccg aaagatccgg tagcgtactg gccgattatg tcttctattc cgcaaacctt
     7981 gctgtatgaa ggttacggta ttcgcaaagg catgtggacg gtgagctggc tacgcgacat
     8041 gttaggcgag tcgttaattc aggatgccaa agcacaggat ctttcaccgg aagatttgct
     8101 caacaaaaaa gcgtcttgtg tgccgccagg ctgtaatggt ctgatgacgg tgctggactg
     8161 gctgaccaat ccgtgggaac cgtacaaacg cgggattatg attggctttg attccagcat
     8221 ggattacgca tggatatatc gttcgatact ggaaagcgtg gcgctgacgc tgaagaacaa
     8281 ttacgacaat atgtgtaatg aaatgaatca ctttgcaaag catgtgatca ttactggcgg
     8341 cggttcgaac agcgatctgt ttatgcagat ttttgccgac gtgttcaacc ttccggcacg
     8401 ccgtaacgcc attaatggtt gtgcaagcct gggggcagcg attaatacgg cggtaggtct
     8461 ggggctatac ccggattacg caacggctgt cgacaaaatg gttcgcgtga aagatatctt
     8521 tatgcctgtt gagagcaacg ccaaacgcta caacgcgatg aataaaggca ttttcaagga
     8581 gttaaccaaa catactgatg taatcctgaa aaaatcgtat gaagtgatgc atggggaatt
     8641 ggggaatgcg gattcgatcc agagctggtc gaatgcgtaa gtgaggaaga ccgggcggga
     8701 aactgcccgg cctgaacata cctgaatggt tatccccgct ggcgcgggga acataagttc
     8761 gacaacttaa aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa
     8821 aaactttaac tagttcggtt tatccctgtt tttacaagga atattaacta cactcgatgt
     8881 ctttagaata aaagac
//
LOCUS       DARNVM010000183         8882 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.183, whole genome shotgun sequence.
ACCESSION   DARNVM010000183 DARNVM010000000
VERSION     DARNVM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.183"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            72..224
                     /gene="hokE"
                     /locus_tag="SI621_004097"
     CDS             72..224
                     /gene="hokE"
                     /locus_tag="SI621_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HEI4066746.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVRDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(346..966)
                     /gene="entD"
                     /locus_tag="SI621_004098"
     CDS             complement(346..966)
                     /gene="entD"
                     /locus_tag="SI621_004098"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HEI4066747.1"
                     /translation="MKTTHTALPFAGNTLHFVKFDPASFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGATALAVVSRQ
                     PIGIDIEEIFSAQTAAELTDNFITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQQIIIRLEDEQFAVHWQIKEKIVITLCQHD"
     gene            complement(1141..3381)
                     /gene="fepA"
                     /locus_tag="SI621_004099"
     CDS             complement(1141..3381)
                     /gene="fepA"
                     /locus_tag="SI621_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HEI4066748.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3624..4826
                     /gene="fes"
                     /locus_tag="SI621_004100"
     CDS             3624..4826
                     /gene="fes"
                     /locus_tag="SI621_004100"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEI4066749.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEITFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4829..5047
                     /gene="ybdZ"
                     /locus_tag="SI621_004101"
     CDS             4829..5047
                     /gene="ybdZ"
                     /locus_tag="SI621_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HEI4066750.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWVVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5044..>8882
                     /gene="entF"
                     /locus_tag="SI621_004102"
     CDS             5044..>8882
                     /gene="entF"
                     /locus_tag="SI621_004102"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HEI4066751.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGIAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQNLRADSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWTEQRRQLPPPASLSPAPLPGR
                     SVSADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRYQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYVQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALVDASYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTTALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFTRQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEHQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMNPERVWSPWIAEL
                     DIYRQDCAHVDIISPGA"
BASE COUNT         1896 a         2392 c         2459 g         2135 t
ORIGIN      
        1 ccctggggct acctacccaa acctgaacaa ctcggatggt agtcccttct tcgcatggag
       61 gcaatataaa catgctgacg aaatatgccc ttgtggcagt catagtgctg tgtttaacgg
      121 tgctgggatt tacgcttctg gtccgcgact cgctgtgtga gtttacggtg aaggaacgta
      181 atattgagtt taaggctgtt ctcgcttacg aaccgaagaa gtagccgttg tgcggggagc
      241 aatccccgca tatccggttg tcaggtcagg atggtaaggc acctgcttta cactttcgcc
      301 cgcggtcagt aatggctgcg ggcgaatcgt accagatgtt gtcaattaat cgtgttggca
      361 cagcgttatg actatttttt cttttatctg ccagtgcaca gcaaactgct catcctctag
      421 tcgaatgatg atctgctgtt tattccagct aattatctga taatccagaa aaccttgagc
      481 ggcctgtatc ttgcttgcct taaatgcgct ctctttggcg gaaaatgcca gtgtcagcgc
      541 cagagaaaag gttaaaccgc attctgcgag tcgctcgtgt tctgctggtg taataaagtt
      601 gtctgtcaat tctgccgctg tttgcgcaga aaaaatttct tcaatatcaa tgccaatcgg
      661 ttgacgagat accacggcta atgccgtagc cccacagtgg ctaatactgc cgtatacccc
      721 cgcaggccac acaggttggc gtagctcgcc gattgcgggc acacatttat agccatattc
      781 ccgcagcgca taaactgcag cgatccgtcc ggctaaatgc tctgttttac gtttacgtcc
      841 agcgtgttgc agttgcgcgt agtgtggcag ccagagtaaa tcctgctcac aaaaactcgc
      901 cggatcgaac ttaacaaaat gcagcgtatt tccggcaaag gggagggcgg tatgcgtagt
      961 tttcatgtcg accatattag agactgatga caaacgcaaa actgcctgat gcgctacgct
     1021 tatcaggcct acgtggtcct gcaatatgtt gaattggcaa gatttttgta ggccggataa
     1081 ggcgttcacg ccgcatccgg catgaactac gcgcactttg tcaacaatct gacgttagca
     1141 tcagaagtga gtattaatgc tcatatacca cgtacgtccc ggctcgttat aggtatacgc
     1201 cccggcaccg gcgatatagt tggctcctgc cagatcgccc gtggtctggg cattacccgc
     1261 acgccacaaa cgtttgtcga acaggttgtc cacgccgccg gtcagactga cattcttcgt
     1321 cacatcccag gtcgcgctca ggccaacaat gctgtacggg ctgatttctt tggtttccgg
     1381 tccaaccgct ggctgacctt tatagttgta cttcttcggc tgctgcttgc cgtaccaggt
     1441 gaaggttgtt tgcatcgaca aatcttcccg tgcctgccag ctcagcgttg agttcaacgt
     1501 atactccggg atgatcgaca aacggtcgcc cgtggttttg ttttcactct tcagcatata
     1561 agtgatgtta ttggtccaca tcaccgtttc gctaaccggt acgtttagcg atccttccag
     1621 accttcaacc accgctttcg gtacgttatc ccactgatag agatcggtgc cgactgcgtt
     1681 ttgccctaca gccacatagc ccgcttcaat cttattgcga taatcgttac ggaaccaggt
     1741 gacgcccgcc agccagccgt cgcgcttgaa ctccagacca atctctttgt taatactggt
     1801 ttccgctttc aggtcatcat taccttgcag atagcagccg cccgcgctgg catagcagcc
     1861 ctgaccttta ctatagagaa tgtagtttgg gttagtctgg tacagactcg gcgctttata
     1921 agcacgggca atgcccattt tcagagtgaa gtcatcgcct aaaccttgcg agatgttcag
     1981 cgccggactc cagttattgc caacaatgct gtgatgatcg aaacgcagcc ccggcgttac
     2041 gatggtgctg tcagtcagct ccatattgtt ttcggcaaac agcgagaaaa tttctgcttt
     2101 tgaatatggg ctacggtctg tcgcactcac accatcaata gcaccgccgg tattcgtccc
     2161 agtgagcgcc tgggtattgg aactcaagtc cttcatccgt tgctgattcc actccgtacc
     2221 cagcgtcagc gtctgattaa cgaggaaatc aatcggcagg ttaacttcgc tgtgcagcat
     2281 cacgtcatcg agatcgatat cgacgaaatc ctgtgtcgct ttttcgttaa atttcccttc
     2341 tgtgccgccc gccagacctt ccggaatacg cgagttacgg gtgtgttcgt actgcaccca
     2401 gttgctggtg gtcacgccgt tatcccagcc gccgttccat gtcagcgagt agttctggcg
     2461 atacagacgg ttggtttcat cgccatattt cgagcgggta taggagtcag agttggtgtt
     2521 ctgggtgtcg ccagcataca ggttaccctg gcggctgtag cccgcttcca gttcgagtga
     2581 ttgcagaggc gcgaagtccc agcgcactac gccattaata tctttgttaa ttaccccttc
     2641 gcgcccggct ggtaacgtcg tggcatacgt tccggcacgc gcggactgat ggccctggtt
     2701 gatatcccac gcgtcagcct gggttttgtc gaggttgcca tacaagcgga agctgaattc
     2761 gtcgcccagc ggaccggtca ggctgaagtt ggtgcgttta gtggcacctt cctctttatg
     2821 ttctggcgca ttgaaataag cgtcccagga gccgtgccac tcaccgctgc cttttttggt
     2881 aatgatgtta accacgccgc ccgccgcgcc gttgccataa cgcgcagctg ccggaccacg
     2941 cagaacttca atacgttcaa tcatttcagg tggcacccag gaagtatcac cacgggtatc
     3001 gcgctcgcca cgccagccct gacgcaccga gttacggctg cttaccggtt tgccgtcaat
     3061 caaaatcagc gtgttttccg gacccatgcc gcgaatatca atctggcggt tattaccacg
     3121 ctgaccactg gtggagttgc cggtcaggtt aacgcctggc atggtacgga tgatctccga
     3181 cacatcgcgg gcaaccgggt ttttgcggat ttcatctgcg gtgatggtcg aaacgccagg
     3241 tgcctgcaag ttctgctcgg cggcggtaac gacaatggta tcgtcatgtg aaacaggagt
     3301 atcggtcggc tcttgtgcct gcgctacccc ataaatcccc agattgacca acaaggccag
     3361 ggaatgaatc ttcttgttca ttgttttatt cctgcatttt tgccacgaat tgcaactgtc
     3421 gggcatggtc gtcatcaaca cgacgcatcc cgctaccgcg aaaacctttg atcctgaaag
     3481 acacgcagtg cagttggtta attaatgtcc gcgcttccca cggcgcgcca ttacgctatt
     3541 gcaaatgcaa atagttatca ataatattat caatatattt ctgcaatcaa taaaaaattg
     3601 cacagtaaac atggggttat ggtgtgacgg cgttaaaagt aggaagtgag agctggtggc
     3661 agtcgaaaca tggcccggaa tggcagcgtc tgaatgacga aatgtttgag atcactttct
     3721 ggtggcgtga tccccagggt tctgaagaat actcgacgat aaagcgcgta tgggtctaca
     3781 tcactggtgt gaccgatcac catcagaaca gccagcccca gtcgatgcag cgaattgcag
     3841 gtaccaacgt ctggcagtgg acgacacaac ttaatgccaa ctggcgcggc agctactgtt
     3901 ttattcccac cgaacgcgat gacatttttt ctgcaccatc ccccgatcgc ctcgaattac
     3961 gtgaaggctg gcgaaaacta ttgccccagg cgatagccga tccgctgaat ccacaaagct
     4021 ggaaaggcgg gcgagggcac gctgtttctg cactcgaaat gccgcaagcg cccctacaac
     4081 cgggatggga ttgtccgcaa gcgccagaaa cgcctgccaa agaaattatc tggaaaagtg
     4141 agcggttgaa aaattcacgc cgtgtatgga tttttaccac cggcgatgca acagcaggag
     4201 aacgcccgct ggcagttttg ctcgatggcg aattttgggc gcagagtatg cctgtctggc
     4261 ctgtgctgac ttcgttgacc catcgtcagc aacttcctcc cgccgtgtat gtgttgatcg
     4321 acgctattga caccacgcac cgcgcccacg aactgccgtg taatgcggat ttctggctgg
     4381 cagtacagca agagttatta cccctggtga aagctattgc cccttttagc gatcgcgccg
     4441 atcgcaccgt ggtcgccggg cagagttttg gtggactttc cgcgctgtat gccggactgc
     4501 actggcctga acgctttggc tgtgtattaa gccagtcagg atcgtactgg tggcctcatc
     4561 ggggcgggca gcaagaaggt gttttacttg agcaacttaa agctggtgaa gttagcgccg
     4621 aaggtctgcg cattgtgctg gaagcgggta ttcgcgagcc gatgatcatg cgggccaatc
     4681 aggcgctgta tgcgcaatta caccccataa aagaatccat tttctggcgt caggttgacg
     4741 gcggacatga tgcgctttgt tggcgcggtg gcttgatgca ggggctaatc gacctctggc
     4801 aaccactttt ccatgacagg agttgaatat ggcattcagt aatcccttcg atgatccgca
     4861 gggagcgttt tacattttgc gcaatgcgca ggggcaattc agtctgtggc cgcaacaatg
     4921 cgccttaccg gcaggctggg tcgttgtgtg tcagccgcag tcacaggcgt cctgccagca
     4981 gtggcttgaa gcccactggc gtactctgac accggcgaat ttcacccagt tgcaggaggc
     5041 acaatgagcc agcatttacc tttggtcgcc gcacagcccg gcatctggat ggcagaaaaa
     5101 ctgtcggatt taccctccgc ctggagcgtg gcgcattacg ttgagttaac cggagaggtt
     5161 gatgcgccac tactggcccg cgcggtggtt gccggaatag cgcaagcaga tacgctgcgg
     5221 atgcgtttta cggaagataa cggcgaagtc tggcagtggg tcgatgatgc gctgacgttc
     5281 gaactgccag aaattatcga cctgcgaacc aacattgatc cgcacggtac tgcgcgggca
     5341 ttaatgcagg cggatttaca gcaaaatcta cgtgccgaca gcggcaaacc gctggtcttt
     5401 catcagctca ttcaggtggc agataaccgc tggtactggt atcagcgtta tcaccatttg
     5461 ctggtcgatg gcttcagttt cccggccatt acccgccaga tcgccaatat ttactgcgca
     5521 tggctgcgtg gcgaaccaac gcctgcttcg ccgtttacgc ctttcgctga tgtcgtggaa
     5581 gagtaccagc aataccgcga aagcgaagcc tggcagcgtg atgcggcatt ctggacggaa
     5641 cagcgtcgtc aactgccgcc gcccgcgtca ctttctccgg cacctttacc ggggcgcagc
     5701 gtctcggcag atattctgcg cctgaaactg gagtttaccg acggggaatt ccgccagctg
     5761 gctacgcaac tttcaggtgt gcagcgtacc gatttagcac ttgcgttggc agccttgtgg
     5821 ctggggcgat tgtgcaatcg catggactac gccgccggat ttatctttat gcgtcgactg
     5881 ggctcggcgg cgctgacggc taccggaccc gtgctcaacg ttttgccgtt ggggattcac
     5941 attgcggcac aagaaacgct gccggaactg gcaacccgac tggcagcaca actgaaaaaa
     6001 atgcgtcgtt atcaacgtta cgatgccgaa caaattgtcc gtgacagcgg gcgagcggca
     6061 ggagaggaac cgctgttcgg tccggtactc aatatcaagg tatttgatta ccaactggat
     6121 attcctggtg ttcagacgca aacccatacc ctggcaaccg gtccggttaa tgaccttgaa
     6181 ctggccctgt tcccggatga acacggtgat ttgagtattg agatcctcgc caataaacag
     6241 cgttacgatg agccaacgtt aatccagcat gctgaacgcc tgaaaatgct gattgcccag
     6301 ttcgccgcgg atccggcgct gttgtgcggc gatgtcgata ttatgctgcc aggtgaatat
     6361 gtgcagctgg cgcagatcaa cgccactcag gttgagattc cagaaaccac gctgagcgcg
     6421 ctggtggcag aacaagcggc aaaaacaccg catgctccgg cgctggttga tgcgagttac
     6481 cagtttagct atcgggaaat gcgcgagcag gtggtggcgc tggcgaatct gctgcgtgag
     6541 cgcggcgtta aaccaggcga cagcgtggcg gtggcgctac cgcgctcggt ctttttgact
     6601 ctggcgctac atgcaattgt tgaggctggt gcggcctggt taccgctgga taccggctat
     6661 ccggacgatc gcctgaaaat gatgctggaa gatgcgcgtc cgtcgctgtt aatcaccacc
     6721 gacgatcaac tgccgcgctt tgccgatgtt ccagatttaa ccagcctttg ctataacgcc
     6781 ccgcttacac cgcagggcag tgcgccgctg aaactttcac aaccgcacca cacggcttat
     6841 atcatcttta cctctggctc caccggcagg ccgaaagggg taatggtcgg gcagacggct
     6901 atcgttaacc gcctgctttg gatgcaaaac cactacccgc ttacaggcga agatgtcgtt
     6961 gcccaaaaaa cgccgtgcag ttttgatgtc tcggtgtggg agtttttctg gccgtttatt
     7021 gccggggcta aactggtgat ggctgaaccg gaagcgcacc gcgacccgct cgctatgcag
     7081 caattctttg ccgaatatgg cgtaacgact acgcactttg tgccgtcgat gctggcggca
     7141 tttgttgcat cgctgacgcc gcaaaccgct cgccagaatt gcgcgacgtt gaaacaggtt
     7201 ttctgtagtg gtgaggcctt acccgctgat ttatgccgcg aatggcaaca gttaaccagc
     7261 gtgccgttgc ataatctata tggcccgacg gaagcggcgg tagatgtcag ctggtatccg
     7321 gcttttggcg aggaactggc agaggtgcgc ggcagtagtg tgccgattgg ttatccggtg
     7381 tggaatacgg gcctgcgtat tcttgatgcg atgatgcatc cggtgccgcc aggtgtggcg
     7441 ggtgatctct atctcactgg catacaactg gcgcagggct atctcggacg ccccgatctg
     7501 accgccagcc gctttattgc cgatcctttt gccccaggtg aacggatgta ccgtaccgga
     7561 gacgttgccc gctggctgga taacggcgcg gtggagtacc tcgggcgcag tgacgatcag
     7621 ctaaaaattc gcgggcagcg tatcgaactg ggcgaaatcg accgcgtgat gcaggcgctg
     7681 ccggacgttg aacaggccgt gacccacgcc tgcgttatta accaggctgc cgcaacggga
     7741 ggcgatgcgc gtcagttggt tgggtatctg gtgtcgcaat caggcctgcc gttggatacc
     7801 actgcattgc aggcgcagtt gcgcgaaaca ttgccaccgc atatggtgcc cgtggttctg
     7861 ctgcaacttc cgcagttgcc gcttagcgcc aacggcaagc tggatcgcaa agccttaccg
     7921 ttgcctgaac tgaaggcaca agcgccgggg cgtgcgccga aagcgggcag tgaaacgatt
     7981 atcgccgcgg cattctcgtc gttgctgggt tgtgacgtgc aggatgccga tgctgatttc
     8041 ttcgcgcttg gcggtcattc gctactggca atgaaactgg ctgcgcagtt aagtcggcag
     8101 tttacccgcc aggtgacgcc ggggcaggtg atggtcgcgt caactgtcgc caaactggca
     8161 acgattattg atggtgaaga agacagctcc cggcgcatgg gattcgaaac cattctgccg
     8221 ttgcgtgaag gtaatggccc gacgctgttt tgtttccatc ctgcgtccgg ttttgcctgg
     8281 cagttcagcg tgctctcgcg ttatctcgat ccacaatggt cgattatcgg cattcagtcg
     8341 ccgcgcccca atggccccat gcagaccgcg gcaaatctgg atgaagtctg cgaagcgcat
     8401 ctggcaacgt tacttgagca tcaaccgcac ggcccttatt acctgctggg ttattcgctg
     8461 ggcggtacgc tggcgcaggg tattgcggcg cggctgcgtg cccgtggcga acaggtggca
     8521 tttcttggct tgctggatac ctggccgcca gaaacgcaaa actggcagga aaaagaggct
     8581 aatggcctgg acccggaagt gctggcggag attaaccgcg agcgcgaggc cttcctggcg
     8641 gcacagcagg gaagtacttc aacggagttg tttaccacca ttgaaggcaa ctacgctgat
     8701 gctgtgcgcc tgctgacgac tgctcatagc gtaccgtttg acggtaaagc gacgctgttt
     8761 gttgctgaac gcacgcttca ggaaggtatg aatcccgaac gcgtctggtc gccgtggata
     8821 gccgagctgg atatctatcg tcaggattgt gcgcatgtgg atattatctc tccaggggca
     8881 tt
//
LOCUS       DARNVM010000184         8878 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.184, whole genome shotgun sequence.
ACCESSION   DARNVM010000184 DARNVM010000000
VERSION     DARNVM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.184"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            85..909
                     /gene="gadX"
                     /locus_tag="SI621_004103"
     CDS             85..909
                     /gene="gadX"
                     /locus_tag="SI621_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HEI4066752.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHNCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGNFYGTDRSTEGIRL"
     gene            1278..2006
                     /gene="gadW"
                     /locus_tag="SI621_004104"
     CDS             1278..2006
                     /gene="gadW"
                     /locus_tag="SI621_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HEI4066753.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2369..5482)
                     /gene="mdtF"
                     /locus_tag="SI621_004105"
     CDS             complement(2369..5482)
                     /gene="mdtF"
                     /locus_tag="SI621_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HEI4066754.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5507..6664)
                     /gene="mdtE"
                     /locus_tag="SI621_004106"
     CDS             complement(5507..6664)
                     /gene="mdtE"
                     /locus_tag="SI621_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HEI4066755.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6724..7002
                     /locus_tag="SI621_004107"
     CDS             6724..7002
                     /locus_tag="SI621_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066756.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLTSPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7003..7530)
                     /gene="gadE"
                     /locus_tag="SI621_004108"
     CDS             complement(7003..7530)
                     /gene="gadE"
                     /locus_tag="SI621_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HEI4066757.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8329..>8878)
                     /gene="hdeD"
                     /locus_tag="SI621_004109"
     CDS             complement(8329..>8878)
                     /gene="hdeD"
                     /locus_tag="SI621_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HEI4066758.1"
                     /translation="ILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTV
                     VGALLICSGVALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAF
                     IAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLV
                     GIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2379 a         2062 c         2073 g         2364 t
ORIGIN      
        1 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaata aacagtaata
       61 tgtttatgta atattaagcc aactatgcaa tcactacatg ggaattgtct aattgcgtac
      121 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggcggt
      181 gatctggttt ttgcggatgc aagccaaatt caagtggata agtgtgttga gaatttcgta
      241 ttagtttcaa gagacacgct ttcattattt ctcccgatgc tcaaggagga ggcattaaaa
      301 cttcatgcac ataaaaaagt tccttcatta ctcgttcata actgtaccag agatattcct
      361 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      421 cgtaaaaggg cattaatctt tgcgttgtta tctgtttttc ttgaggatac gcagtttata
      481 ccgttacttc tgaacgtgtt gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat
      541 aataatatcg cccatgagtg gacattagcc cgaatcgcta gcgaattgtt gatgagtcca
      601 agcctgttaa agaaaaaatt gcgcgaagaa ggtacgtcat attcacagtt gctcactgag
      661 tgtaggatgc agcgtgcttt acaacttatt gttatatatg gtgtttcaat taagcgagtt
      721 gcagtatcct gtggatatca cagcgtgtcg tatttcattt acgtttttcg aaattattat
      781 gggatgacgc ccacagagta tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg
      841 gcggcaagta tggctgcgca agggaatttt tacggcactg accgttctac ggaaggaata
      901 agattataga gttttactca gacataaaaa aaacccggca taggggaccg ggaagaggat
      961 agtctgccgt ctccagacta ataaaccgtt ataacactcc ctgttggcac gggaaacttt
     1021 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1081 acataagcta tacgctgtgc gaaaatatat tcgtgttgca tttacttatt atcaattaac
     1141 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1201 ctaagaaaca gtctggcatc atttcattag tatactgaaa ttgaaataat cgcagtaatg
     1261 aaatataagg gatagtcatg actcatgtct gctcggtgat cctcgttcgt cgttcattcg
     1321 atatttatca tgaacagcaa aaaatatcgt tgcacaacga gagtatcctg ttactggata
     1381 aaaatttggc agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg
     1441 agctgacagt ttgccattac ttacaaaata ttcgtcagat accacgcaat ttagggttac
     1501 atagcaaaga ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt
     1561 ttgatagttt caatgactcc ggggtaaatt caccgatact gagcaaaatg ctctatcttt
     1621 cctgtttatc gatgttttct cataagaaag aactgatccc cttacttttc aatagtatca
     1681 gtactgtttc aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc
     1741 tgcgcgatat cgcggaaaga atgtatacca gcgagagtct aatcaaaaaa aagttgcagg
     1801 atgaaaatac ctgtttcagt aaaatattac tcgcctccag gatgtcgatg gccagacgat
     1861 tactcgagtt acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta
     1921 catcgtactt tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc
     1981 aacattcgcc aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca
     2041 cacctgcttc atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct
     2101 ttcgcagttt gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga
     2161 ttattaagct gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgtttaaat
     2221 tgatgacgaa tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc
     2281 tgacggttag ctggttgttg agtggtgaat ggttagcggg aaagagtaag gctgaaccct
     2341 cacgttcaac cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt
     2401 ccactacaac gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc
     2461 cgcccatcac gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa
     2521 ccagcggcag cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca
     2581 tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa
     2641 cggcaaattc gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac
     2701 caacctggaa gtagacgtca ttacttaagc cgcgtagatc ggtggccagt aatgcgccaa
     2761 cgacgcctaa cggtacaacc aacatcaccg agaacggaat tgaccagctc tcataaagtg
     2821 cggcgagggc gaggaacacc acgaccagtg aaatggcata cagcgcggga gcctgatttg
     2881 aggataacgc ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt
     2941 tagcgaccag gtctgccata aatttcatgg cgtcaccggt acttttcccg gccgccgctt
     3001 cacctaaaat ctccattgac gggatgccgt tgtagcgttc cagtcgcggt gaaccatagg
     3061 tccattcggt agacgaatag gccgaaagcg gtgccatcgt gccagaggcg ttgcgtacat
     3121 accattggtt gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt
     3181 ttttcacccg cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa
     3241 ttgtctggtt gatatcagac agcgccacac ccatagcctc ggctttcgcg gcgttgacgt
     3301 tcactttgaa catcggcgta tcttccaggc cgttcgggcg tacaccggtg acttgattcg
     3361 gtgattgcgc tgccagtgat aacagctcgt ttcgcgcctg ggttagtttt tcgtgcccca
     3421 ggttaccgtt gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg
     3481 cgggtaagtt gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc
     3541 gctgaatgat cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac
     3601 tgataaacgc caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa
     3661 ataccgactg aacattatct ttctctttag tcagataata atccgtcacc tgttgcagca
     3721 ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc
     3781 cctgatcctc ttctggtaag aaagaggtcg gcgtgcgcag gaacagcacc gccatcccgg
     3841 cgcaaatcag caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtac
     3901 tgtcggtata gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt
     3961 taggtttgtg accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca
     4021 ggctcattgc cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc
     4081 ggtagatctc cccggttgca ccgctcataa aggccatcgg cataaacact gcggaaagaa
     4141 caacggcaat cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct
     4201 tcggcggtag tttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt
     4261 catccaccag taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga
     4321 aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg
     4381 ggatgattgt ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga
     4441 tagcctcaac cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt
     4501 cgtaaggata aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct
     4561 ctttgactgc ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc
     4621 cggcagcagg tttgccgtta tagcgcgcca cggtggaata gtcttccgct ccaagttcga
     4681 cgcgggcgac atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt
     4741 tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct
     4801 ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttctgcacct
     4861 taatctggga aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca
     4921 gccagatacg catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac
     4981 ggcttagcgg gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc
     5041 tgccgttatc agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc
     5101 taatcccctg ctgctgcacc gcttcaggta atgaaggcat agcgagctgc agtttatttt
     5161 gcacctgaac ctgtgcgata tcaggcgatg tcccagtctc gaaggtcaga gtgatagagg
     5221 cattgcccgc cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat
     5281 tttgctcaat cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg
     5341 ttgcgctgac ggttatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca
     5401 tgatcgccag accgcctgca agcatcataa taatggcaag tacccaggca aaaaccgggc
     5461 gatcaataaa atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt
     5521 cggtgctggc gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc
     5581 gttgcaaacc ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact
     5641 ggtcgccgat ggctttgctg gcttcgattt cgcgtagctg cacgacatcg tctttatcca
     5701 gaatgagcgc cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta
     5761 atacattctg gcggctacct tcatccacta atgccgtaac gtacatgcca ggcagcaagt
     5821 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg
     5881 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
     5941 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
     6001 tacgcaagaa atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
     6061 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
     6121 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcaaattg atcgtcgcct
     6181 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
     6241 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
     6301 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgtgcct ttggcggagt
     6361 tcagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
     6421 tatcgccttc gataaagttg cgtttaatga taataccgcc aacctgggga cgtatctcgg
     6481 caacttcata aggaacggtt ctaccgggca attcgctcaa tacattgacc gaaccggggg
     6541 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
     6601 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
     6661 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
     6721 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     6781 aggcatagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     6841 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     6901 aaagtcgttt taacctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     6961 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     7021 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     7081 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     7141 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     7201 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     7261 gctgacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     7321 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     7381 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     7441 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     7501 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     7561 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     7621 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atgcttcaga
     7681 tttatccatc acacactcca tatattgatc ctaagtaaaa ttcctggttg ttatcagttt
     7741 gtaaacggca aacaaaaact taacgtttac ggattactgc ccaagaataa gttatcaata
     7801 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     7861 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     7921 aaattacctg agtaaataac tcattcatgt cttcaggttt ttatatagcg tctggagaca
     7981 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     8041 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     8101 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     8161 ccatggaatg aaaaaaatta gatgaaattc agtaggttga aataatcatc agcaggtaat
     8221 tatttcaatg atagtgcgca attgatctac aacgctgtgt agcggagaga gtattaatcg
     8281 gatcatagtc acatcaagtg actatgatcc gggtgacaac aggaataatt attgctgctt
     8341 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caatacccac
     8401 cagtgtggaa accaaagtta cagacaccat cggcgttgca ccaaggaata tccatgcaat
     8461 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     8521 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     8581 aatggcaaaa atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc
     8641 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     8701 tccaacaata agcgcaacgc ccgagcagat taataatgca cccactaccg tgcttaaaat
     8761 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcaacacggc
     8821 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaatt
//
LOCUS       DARNVM010000185         8738 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.185, whole genome shotgun sequence.
ACCESSION   DARNVM010000185 DARNVM010000000
VERSION     DARNVM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.185"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            61..165
                     /locus_tag="SI621_004110"
     CDS             61..165
                     /locus_tag="SI621_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HEI4066759.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            476..2674
                     /gene="speF"
                     /locus_tag="SI621_004111"
     CDS             476..2674
                     /gene="speF"
                     /locus_tag="SI621_004111"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HEI4066760.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIIEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENGPGILVTQSVHKQQAGFSQTSQIHKKDNHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2671..3990
                     /gene="potE"
                     /locus_tag="SI621_004112"
     CDS             2671..3990
                     /gene="potE"
                     /locus_tag="SI621_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HEI4066761.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4047..5687)
                     /gene="pgm"
                     /locus_tag="SI621_004113"
     CDS             complement(4047..5687)
                     /gene="pgm"
                     /locus_tag="SI621_004113"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEI4066762.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASISGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5713..6258)
                     /gene="seqA"
                     /locus_tag="SI621_004114"
     CDS             complement(5713..6258)
                     /gene="seqA"
                     /locus_tag="SI621_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HEI4066763.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6443..7207
                     /gene="ybfF"
                     /locus_tag="SI621_004115"
     CDS             6443..7207
                     /gene="ybfF"
                     /locus_tag="SI621_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEI4066764.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWEQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7347..7640
                     /gene="ybfE"
                     /locus_tag="SI621_004116"
     CDS             7347..7640
                     /gene="ybfE"
                     /locus_tag="SI621_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HEI4066765.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7780..8310
                     /gene="fldA"
                     /locus_tag="SI621_004117"
     CDS             7780..8310
                     /gene="fldA"
                     /locus_tag="SI621_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HEI4066766.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8520..8606
                     /gene="uof"
                     /locus_tag="SI621_004118"
     CDS             8520..8606
                     /gene="uof"
                     /locus_tag="SI621_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HEI4066767.1"
                     /translation="MIRIISRANSVTSRNEVNRLVTGQIPHD"
     gene            8599..>8738
                     /locus_tag="SI621_004119"
     CDS             8599..>8738
                     /locus_tag="SI621_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEI4066768.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMG"
BASE COUNT         2104 a         2163 c         2201 g         2270 t
ORIGIN      
        1 tagccatttc atggatagaa taactctacc ttcaactgac acagcaagag gtaaaggtaa
       61 atggaaaata ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt
      121 gcccatcgta atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg
      181 gcaccccaac gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtatccctc
      241 ggtgctcata tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa
      301 cttgaggtat gacgagtata gccagttacc gggctggtct gggttattgc atctacaaaa
      361 agcaaactac tgatttattt atcagcggtg gggttttgct ttttttccgg cgtgatcgat
      421 ttctcctttg agaaattgag gacctgctat tacctgaaat aaagagatga acaaaatgtc
      481 agaattaaaa attgccgtta gtcgttcttg cccggattgt ttttcaactc atcgcacatg
      541 cgtgaatatt gacgaaagta attatattga cgttgccgcc attattttat cggtcaatga
      601 tgtggaacgt ggaaaactcg atgaaataga cgctactggc tatggcattc ctgtttttat
      661 tgcaacggaa aatgaagaac gtgtacccgc agaatatctg ccacgtattt ctggtgtctt
      721 cgaacattgc gaatcacgta aagagtttta tggacgtcag ttagaaaccg ctgcaagcca
      781 ttatgaaacg caactgcgcc cgcccttctt ccgcgcactg gtcgattatg ttaatcaggg
      841 caatagtgct tttgactgcc ctggtcatca ggggggtgag tttttccgtc gccatccggc
      901 aggcaatcag ttcgtggaat actttggtga ggcgctgttc cgtgctgact tgtgcaacgc
      961 cgacgtagcg atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca
     1021 tgcggctaaa gtctttaatg ccgataaaac ctatttcgtt ttaaacggta cttcatcttc
     1081 taataaagtg gtcttaaacg cattgctgac gccgggcgat ctggtgttgt ttgaccgcaa
     1141 taaccataaa tctaaccacc acggtgcgtt gctacaggct ggcgcgacac cggtttatct
     1201 ggaaaccgca cgtaacccgt atggttttat cggtggtatt gacgctcact gttttgaaga
     1261 aagttacctg cgtgagctga ttatagaagt tgcaccgcag cgggcaaaag aggctcgtcc
     1321 tttccgcctc gccgttattc agctaggcac ttacgacggt acgatttata acgcccgtca
     1381 ggtggtggat aaaattggtc atctgtgtga ctacatcctc tttgactccg catgggtcgg
     1441 ctatgagcag tttatcccga tgatggcgga ctgttcacca ctgttgctgg atcttaatga
     1501 gaacggtccg ggtattctgg ttacgcaatc cgtacataaa cagcaggctg gtttttctca
     1561 aacttcacaa atccataaaa aagacaacca catcaaaggg caacagcgtt atgtaccgca
     1621 caaacgcatg aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt
     1681 tgccgcactg gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat
     1741 ggattgtgtg gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat
     1801 tcgtccgttc gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca
     1861 aattgcggtt gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga
     1921 aggctatgca gagaatcaat actttgttga tccgtgcaaa ctgttgctga caaccccagg
     1981 tattgatgca cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa
     2041 tttcctgcgc gaaaatggcg tagtgccgga aaaatgcgat cttaactcca tcctcttcct
     2101 gctgactcca gcagaagata tggccaaact tcagcaactt gttgccctgc tggtacgctt
     2161 cgaaaaactg ctggaatctg atgcgccgct ggcggaagtg ctaccttcca tctacaaaca
     2221 gcatgaagag cgctacgccg gttatacgct tcgtcagttg tgccaggaaa tgcatgattt
     2281 gtatgcccgc cacaacgtga aacaactgca aaaagagatg ttccgtaagg aacacttccc
     2341 acgcgtcagc atgaatccgc aagaagctaa ctacgcctat ttacgcggtg aagtggagct
     2401 ggttcgtctg ccggatgcag aaggccgtat cgctgcagaa ggtgcgcttc cttatcctcc
     2461 gggtgtgctg tgtgttgttc cgggtgaaat ctggggtggt gctgttttgc gttacttcag
     2521 cgctctggaa gaagggatca acctgctgcc aggttttgca ccggagctgc agggtgtcta
     2581 tatcgaagaa catgatggtc gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc
     2641 gcaaagcacc ctgttgaaag gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc
     2701 gtcgttcagt taaccatact gacgatggtc aacatgatgg gctccggtat catcatgctg
     2761 ccgacaaagc ttgccgaagt tgggacaatt tcaattatct cctggctggt gacagccgta
     2821 ggctcaatgg cactggcatg ggcattcgca aaatgcggta tgttcagccg taaatcaggt
     2881 ggtatgggcg gttatgccga gtatgctttc ggtaaatccg gtaactttat ggcgaactat
     2941 acctacggcg tctcactgct gattgctaac gtcgcgattg ctatttcggc ggttggttac
     3001 ggcaccgaac tgctcggcgc gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg
     3061 gtgctgtgga tttgtaccgt ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt
     3121 agcattaccg tgtggggggt cattattccg gtggtaggcc tgtgcgttat cggctggttc
     3181 tggtttagcc cgacgcttta cgttgattcc tggaatccgc atcatgcacc gttcttcagt
     3241 gcggtaggtt cttccatcgc tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt
     3301 gcgaatactg atgtagtgga aaacccggaa cgtaatgtgc caatcgcggt actcggcggt
     3361 acgttaggtg cggcggtgat ttatatcgtc tccaccaacg tgattgccgg gattgtgcct
     3421 aatatggagc tggcaaattc aacggcacca tttggcctgg ccttcgcaca gatgttcacg
     3481 ccagaagtgg gtaaagtcat tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt
     3541 ggctggcagt tcaccattgc ccaggtgttt aaatcttcat ctgatgaagg ctacttccct
     3601 aaaattttct cccgtgtaac caaagtggat gcaccggtgc agggaatgtt gaccattgtg
     3661 attatccaga gcgggttggc gctgatgacg attagcccgt cgctaaacag tcagttcaac
     3721 gtgctggtta acctggccgt ggtgaccaat atcattccgt atattctgtc aatggcggca
     3781 ttagtcatta ttcagaaggt cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt
     3841 gttgcttttg ttggcgcgat gtacagcttt tatgcgctgt actcatccgg ggaagaagcc
     3901 atgctatacg gttccatcgt gaccttcctc ggttggacac tgtatggtct ggtctcacca
     3961 cgctttgaac tgaaaaataa acacggttaa ttaagcaaag caaaaaaggg cgatcttccg
     4021 accgcccttt ttcttattta atgtgtttac gcgtttttca gaacttcgct aacaatctca
     4081 accgcttctt tctcgatctg cttgcgatgc tcttcaccga ggaagctttc gcaataaatc
     4141 ttgtacgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact
     4201 ttcagaccgc tgatagaagc accgttgccc ggtgccgccg tcaggcgcgc ggtgatcggg
     4261 tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt
     4321 tgtgcggaag tcgcggctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc
     4381 agttcgttgt agtgttcctg cggattctta ccggtgacag cggtgatttc cgccgccagc
     4441 agacacataa tgatgccgtc tttatcggtg gaccacggcg tgccgtcgaa acgcaggaag
     4501 gaagcgcctg cactctcttc gccgccaaag ccgaagctgc catcaaacaa accatcaaca
     4561 aaccatttga aacctaccgg gacttctacc agcttacggc ccaaatcatt gaccacacgg
     4621 tcgatcatcg cagatgaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga
     4681 cggtgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct
     4741 gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac
     4801 ttatcacgca gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc
     4861 gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg
     4921 gtcaggttga gattgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc
     4981 agcggatcaa cgccaagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg
     5041 gccagccctt ccacgaacgg ctgcaccaga tcctgctctt tcacatgacc ggatgccatc
     5101 gcttcgtcga gggagatacg cttcacacct ttcaggccat cggccatcag tgcgttggcc
     5161 ctgtcttcca ccactttggt gacgttggta tcagccgggc caccatttgg cgggttgtac
     5221 ttgataccac catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc
     5281 gggccacctt ttttattgtg aaccaggatg gcattggaaa cggcaggcgt tggagtgaag
     5341 ccattgtttt cctgcacaat aacatcaacg ccgttcgctg ccagcacttc cagaacggaa
     5401 atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg
     5461 ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta
     5521 aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc
     5581 gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc
     5641 aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt
     5701 gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat
     5761 cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac
     5821 ccaatacggc gtgccaggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt
     5881 ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg ttgcctcggc
     5941 aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc
     6001 tcgcttttgc tctgcgtatt catccgaaag cagaagttca cgcattgcgc gaaccttgtc
     6061 tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt
     6121 cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc
     6181 ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc
     6241 aacttcaatc gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccagg
     6301 agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga
     6361 ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca
     6421 acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg cacaaaacca
     6481 gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggcagcctcg acaaccttgg
     6541 cgtactagct cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca
     6601 cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg atcttgttga
     6661 taccctggat gcactgcaga tcgataaagc aacatttatc ggtcactcca tgggcggtaa
     6721 agcggtaatg gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgattga
     6781 catcgcgccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg ctatcaacgc
     6841 agtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct
     6901 taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt
     6961 taacgtgccg gtattgtggg aacagtatcc gcatattgtg ggttgggaga aaatcccggc
     7021 atgggatcac cctgccctgt ttatcccagg cggcaattct ccgtatgtta gcgagcagta
     7081 tcgtgatgat ttactggcgc aatttccaca ggcgcgggcg catgtgattg ctggcgcggg
     7141 tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa
     7201 tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg
     7261 cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga
     7321 ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag
     7381 atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg
     7441 aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc
     7501 gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcagaatcgc
     7561 gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc
     7621 gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt
     7681 ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc
     7741 cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt
     7801 ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt
     7861 aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcatat
     7921 gacattctgc tgctgggcat cccaacatgg tattacggcg aagcgcagtg tgactgggat
     7981 gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc gctgtttggt
     8041 tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac
     8101 atcattgaac cgcgtggcgc aaccatcgtt ggtcactggc caactgcggg ctaccatttc
     8161 gaagcgtcaa aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac
     8221 cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag
     8281 ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca tgtctacgcc
     8341 gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta
     8401 cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc agtgccacgt
     8461 ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa
     8521 tgatacgcat tatctcaaga gcaaattctg tcacttctcg taatgaagtg aaccgcttag
     8581 taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa
     8641 gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc
     8701 agtgcggaag atttatacaa acgtctgatc gatatggg
//
LOCUS       DARNVM010000186         8687 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.186, whole genome shotgun sequence.
ACCESSION   DARNVM010000186 DARNVM010000000
VERSION     DARNVM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.186"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(23..3910)
                     /gene="purL"
                     /locus_tag="SI621_004120"
                     /gene_synonym="purI"
     CDS             complement(23..3910)
                     /gene="purL"
                     /locus_tag="SI621_004120"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEI4066769.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGLGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAVQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADRESVEAVLAQHGLADCVHYVGQAVSGDHFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4167..5723
                     /gene="mltF"
                     /locus_tag="SI621_004121"
     CDS             4167..5723
                     /gene="mltF"
                     /locus_tag="SI621_004121"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEI4066770.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5720..6256)
                     /gene="tadA"
                     /locus_tag="SI621_004122"
     CDS             complement(5720..6256)
                     /gene="tadA"
                     /locus_tag="SI621_004122"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEI4066771.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6281..6916)
                     /gene="yfhb"
                     /locus_tag="SI621_004123"
     CDS             complement(6281..6916)
                     /gene="yfhb"
                     /locus_tag="SI621_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HEI4066772.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7125..7973
                     /locus_tag="SI621_004124"
     CDS             7125..7973
                     /locus_tag="SI621_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEI4066773.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT         2134 a         2373 c         2179 g         2001 t
ORIGIN      
        1 gcaaaaccaa tgggctgacg acttacccca actgcttacg cgcattgcgg aaaatgcgca
       61 tccatgggcc atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa
      121 cacgttccgg gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa
      181 taccgttcgg tgaaccgttc gggttagccg ggtaggtttc agtgacttta ccgaagttat
      241 cgacatagcg cagtgccacc agccctttgc tttccagcgc cgccagatgc gccgcatcac
      301 gcacttccac gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc
      361 cctgcaacag cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc
      421 gatcggaggt attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca
      481 ggttagacat catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacgatgga
      541 agaaggttgc aaactcatcg cgtacacggt cattgaacag gatagacttc gcccaacctt
      601 caccggcacc cagcacatca ccgtaggaga aaccgccgca cgcgaccagg gcgtggaaat
      661 cctccaggcc cgtgcgtcca gccagcagat cgctcatatg cacgtcgata gcatcgaagc
      721 ctgcacggtg gaaagctgcc gccatttcaa catgcgagtt cacaccctgc tcacgcagca
      781 cggcaacttt cgggcgcgcg ccagtggcaa taaacggtgc tgccacatct tcgttgatat
      841 caaacgacag tttcacattc aggcccggat cggcgtcgtt ggatttcgcc tggtgctcct
      901 ggtcagcaca ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc
      961 agacacgcaa cgtggtgcgg ctttcgctaa atacagtctg cccgttggca gtaatcacaa
     1021 aatggtcacc ggaaaccgct tgcccaacat aatggacgca atcagcaagc ccatgctgtg
     1081 ccagtaccgc ttcaactgat tcacggtcag cggctcgaac ctgaatcacc gcacccagtt
     1141 cttcgctaaa caatgcagcc agacgatcgt cacccagagt ggcgatatct gcgtcaatgc
     1201 cacaatgacc agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt
     1261 cgtggtacgc gagcagctta cgctgtgcta ccagcgcctg aaccgcgtca tagaaacctt
     1321 tcagttgcgc gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg
     1381 ccagcgccgt tgcgcccagc gcgttattgc ctttgcccag atcaatcagc agtaatgcgt
     1441 tatcttcggt agaaagctgc ggcgtgatgg tgtgacgcac atcttccaca cgggcaaatg
     1501 cagaaatcac cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc
     1561 gggttttcat cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa
     1621 gttcttcgcc cacagcttta acggcttcat acaggcccgc atcttcacca gggtggcctg
     1681 ccgccgccat ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg
     1741 caatattggt taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg
     1801 caaccggcgc acgctcgcca atcgccatcg cttcgccgta gtagctgtcg aggctggcgg
     1861 tagtgaccgc gcagttagcg accggcacct gccacggacc caccatctga tcgcgcgcta
     1921 ccatgccggt tacgctgcgg tcaccaatgg tcaccaggaa agttttctcc gccacggtcg
     1981 gcaggtgcag cacgcgtttc accgcgtcag caatggtaat cccttcacga accagcgcat
     2041 cacccttcgc tttcagcgtt tgtacttcgc gggtcatctt cggcgtttta ccaagcagta
     2101 cgtccagcgg cagatcgatc ggctgattat caaaatgacg atcgtgcaaa gaaagatgca
     2161 gttcttcggt cgcttcacca atcaccgcgt atggcgcgcg ctcacgctta cacagttcgt
     2221 caaacagcgg taactgatcg gcagcaaccg ccagcacgta gcgttcctgg gattcgttac
     2281 accagatttc cagcgggctc atgcccggtt cgtcgctcag gatatcgcgc agttcgaatt
     2341 taccgccgcg cccgccgtcg ctcaccagtt ccggcatggc gttagaaaga ccgcctgcgc
     2401 caacgtcgtg gataaacagg attgggttgg catcaccaag ctgccagcaa cggtcgatca
     2461 cttcctggca gcgacgctcc atctccgggt tgtcgcgctg tacggaagcg aagtcgaggt
     2521 cggcatcaga ctgaccagac gccatggaag acgccgcgcc gccgccaaga ccgatgttca
     2581 ttgccgggcc gccgagaacg accagcttcg caccaacgtt gatctcgcct ttttgtacgt
     2641 gatcggcacg aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca
     2701 gctcttcgcc attgtggctg ttcacttttt cttcataagt acggaagtag ccgttcagtg
     2761 ccggacgacc aaattcgttg ttaaatgccg cgccacccag cgggccttcg gtcatgatgt
     2821 ccagcgcggt gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc
     2881 caggaattcg caggttggaa acggagaaac caaccaaacc agctttcggc ttcgcgccgc
     2941 gtccggtggc accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg
     3001 gagaaatcgc cgtcgggtgg ttgtgggttt caactttcat cagaatatgc gccggttcct
     3061 gatggaaatc gtagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaccctt
     3121 ccatgacggc ggcgttatct ttataagcag agagaacgtg atccggcgta gtttcgaagg
     3181 tgtttttgat catcttaaac agcgatttcg gctgctgttc accatcgata acccagtcgg
     3241 cgttaaaaat tttgtggcgg cagtgctcgg agttcgcctg ggcaaacata tacagttcga
     3301 tgtcgttcgg gttacgacca agctttgtga aagcatcctg caaatagtca atttcatctt
     3361 ccgccagtgc caggccaaga cgcaggttag cgtcgattag cgcctgacgg cccaacccca
     3421 gcaaatcaac gctggtgacc ggagtcggtt gatggtgtgc aaacagctgc tctgcatcat
     3481 ctaaagcaaa aaagaccgtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc
     3541 attgttcatt ggtcagcgta ccggcttcta tatagtaagc cacgccgcgc tcaagacggt
     3601 ttacctgttg cagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga
     3661 tggtgccagg acgcggggtc accagcagga gtttgccttg cggggcgtgg ctggcgagtg
     3721 ccgggccata tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat
     3781 tgaggtcagc aaaatggaca tactcggcgt aaatagtgtg aaccgggagc ctggcagcct
     3841 gaaaacgtgc cagcagtttg ttgattcgga atgccgacaa tgcaggcgaa ccacgcagaa
     3901 tttccatcat aagtctctcg tcttctaagc tttcggtgta cccaaggggg gaaacgggcg
     3961 tcattataaa gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg
     4021 cgaattagca acgcgtgccg ccaatggctg taataagttg ccaactggcg caggtttacg
     4081 caaaatgccg ctcatttatg agtaaacctt tcactattat tacgtttttt caagctggga
     4141 cgcgcacgac acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg
     4201 gcattctggc actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag
     4261 ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata
     4321 ctcccctgac gtataacgaa atcaacggga aaccttttgg cctggattac gaactggcga
     4381 aacagtttgc cgattacctc ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc
     4441 agctgtttga cgacctcgat aatggtaacg ccgacctgct ggcggcaggc ctggtctata
     4501 acagtgagcg tgtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac
     4561 tggtttataa agtgggtcag tatcgcccac gtacactggg caacctgacg gcggagcaac
     4621 tcaccgttgc accgggtcat gtggtggtta acgatctcca gaccctgaaa gaaacaaaat
     4681 tcccggaatt aagttggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg
     4741 tcatcgaagg aaaactcgat tacaccattg ctgattctgt tgccattagc ctgtttcagc
     4801 gcgttcatcc ggaactcgcc gtagcgctcg acattaccga tgaacaaccg gtgacctggt
     4861 ttagcccatt agatggcgat aatacccttt ccgccgctct gctcgatttc ttcaacgaaa
     4921 tgaatgaaga cggtacgctg gcacgcattg aagagaaata cctggggcat ggcgatgatt
     4981 ttgattacgt cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa
     5041 agcccctgtt tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt
     5101 atcaggaatc gcactgggat gcgcaggcca cttcaccgac gggtgtgcgc ggcatgatga
     5161 tgctaaccaa aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga
     5221 gcatcagcgg cggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc
     5281 cggagaacga acggatttgg tttgccctcg ccgcgtacaa tatgggctat gcgcatatgc
     5341 tggatgcccg cgctctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa
     5401 aacagcgtct gcctttactt agccagaaac cctattacag caagctgact tacggctacg
     5461 ctcgtgggca tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg
     5521 tgggttatct gcaggagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg
     5581 attatccggc ggtatcgcct acggagctgg gcaaagagaa atttcctttt ctctcgtttc
     5641 tttcccagtc gtcatcaaac tatttgaccc actctccctc tctgctgttt tccaggaaag
     5701 ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt
     5761 cctggcggcg catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc
     5821 cttcggtaat ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc
     5881 ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga
     5941 tcatcgctcc ggcacacatt acacagggtt caagcgtgac atacaacgtg gcgtcgatca
     6001 gacgataatt ttgcatcacc agaccgccct gccgcagagc catgatttct gcatgtgcgg
     6061 tgggatcatg tcgaccaatc ggacggttcc agccttcgcc aataacacga ttgttatgca
     6121 ctaataccgc gccgaccggc acttcccgct catcccaggc acgctttgcc agcgtcagcg
     6181 cgtgacgcat ccagtattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta
     6241 taaaagcgcg gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt
     6301 caccgcgcgg ggttactcgc caacgatgct gacagaaata aagcagcggg ttgtcctgat
     6361 tactgtcgct atagccactg tacaaccgca gtggcgtgcc gattttacgc tccagttgcg
     6421 cgaccttctc atgcccgagg caacgcatcg tcaataccca accaccatag ccacgctgaa
     6481 tctgactggc gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa
     6541 ccagcggctg cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg
     6601 tggttaatcg ctcctgaacc agcggaaagg cggtaacatt gtcacgaaac cagcgtacga
     6661 aatcggcctg caacgcctgc aaacgcgctt cattgtgacc aaaagtgcat ccccacagaa
     6721 gcagactcat cggccagcgt gccgcacggc cttttatcaa taacgcaatg gctatgatcg
     6781 gcaacaacgg caggacaagc aacgcattca gcggttggcg ccgcagtaaa taacgcaaaa
     6841 aactgccgaa catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac
     6901 gctcgtgagt tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac
     6961 tacatagcct aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa
     7021 ctggaatttt taattcatgg aaattagcgg caatggaata taaaattcac tcgcgtttgt
     7081 ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc
     7141 gtatccgtca gcgttaccag gggcttgccc agagtgataa aaaactggcg gattatctgc
     7201 tgctacaacc tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag
     7261 tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc
     7321 ttaagttggc gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc
     7381 ataaccaaat ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa
     7441 atactgccgc tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg
     7501 taacaatgtt gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg
     7561 tggcgcaaaa ctttgcctgg aagctgatga agattggctt caatgctgcc gcagtgcgcg
     7621 atatgcatgc gctactcgct accgtacagg cgtcgtcccc tgacgatctg ttattagcca
     7681 tttcttacac cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg
     7741 gcggaaaagt gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc
     7801 attgcctgta taccattgcc gaagaacaag cgacaaacag tgcttcaatc tctgcttgtc
     7861 acgcgcaggg aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac
     7921 tggctccaga acgtattcgt cacagtgaag cgctggtgaa aaaactggtc tgagtaaaga
     7981 atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgctgtta
     8041 atcactaaaa aatgcatcaa ttgtgatatg tgtgaaatca aataaaaaca catttatttt
     8101 catagtgata cagcaatgtg ttattgaatg tgttgttgga tttatggtgc aaaaaaattt
     8161 tgcataagtt cagcaacaca aagatccccc ccctttttct ataacggttc atttgagact
     8221 cccccttgac tccctcttcg aaattgaaga ccatcacctg tacataatta cagtcatgga
     8281 taatattatg cgcagaaaag agtcaaacgt tagcagtttg ccggaactca ccaattttga
     8341 ggtaagctat tctctggtta ccaatgaagt ttacttgtct gcatccttca ttgataacat
     8401 ggcctgtata ccaaactggc ccttacaaga atttccggat caactaatat gcatttctcg
     8461 agcaaaagct gttgccctaa tcgaagagct ccagaagacc atcaactata tggatgcagg
     8521 aatagatcga agttcaggta gccttctcca gtagctgacg agttttataa taatttgata
     8581 tatatatatc gttcaatatt agcgagtact tataatgagt gaatttagta atcttttaaa
     8641 tagcatacct ggatggttat cctcctccct cacagctcta gttggta
//
LOCUS       DARNVM010000187         8518 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.187, whole genome shotgun sequence.
ACCESSION   DARNVM010000187 DARNVM010000000
VERSION     DARNVM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.187"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            62..832
                     /locus_tag="SI621_004125"
     CDS             62..832
                     /locus_tag="SI621_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HEI4066774.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTVAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            823..1296
                     /locus_tag="SI621_004126"
     CDS             823..1296
                     /locus_tag="SI621_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HEI4066775.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1403..1942
                     /gene="dnaT"
                     /locus_tag="SI621_004127"
     CDS             1403..1942
                     /gene="dnaT"
                     /locus_tag="SI621_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HEI4066776.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1945..2682
                     /gene="dnaC"
                     /locus_tag="SI621_004128"
     CDS             1945..2682
                     /gene="dnaC"
                     /locus_tag="SI621_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HEI4066777.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2731..3225
                     /locus_tag="SI621_004129"
     CDS             2731..3225
                     /locus_tag="SI621_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HEI4066778.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3479..5770
                     /gene="opgB"
                     /locus_tag="SI621_004130"
     CDS             3479..5770
                     /gene="opgB"
                     /locus_tag="SI621_004130"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HEI4066779.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLFGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5910..6932)
                     /gene="lgoD"
                     /locus_tag="SI621_004131"
     CDS             complement(5910..6932)
                     /gene="lgoD"
                     /locus_tag="SI621_004131"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HEI4066780.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIANLKNGQQVAVIPYVACQQCPTCKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEYFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7071..7985
                     /locus_tag="SI621_004132"
     CDS             7071..7985
                     /locus_tag="SI621_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4066781.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8200..>8518
                     /locus_tag="SI621_004133"
     CDS             8200..>8518
                     /locus_tag="SI621_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066782.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGP"
BASE COUNT         2154 a         2203 c         2061 g         2100 t
ORIGIN      
        1 gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt
       61 tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg gattatttct
      121 tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc
      181 gttgggaatg gacagcgtcg aaagttctat ctcttcgaac gccatagtgc tgacaaccat
      241 taaagatggg caatgcctga catcgacacg aaaaaatcac gatcgcggca ttaatatgca
      301 tgtggtgact gaagtccagc acattgtgat tctggcggag catcatctgc tggattacaa
      361 aggtgtagag aaacgattta gccaaattca gccattacgt tacccgagat ggctggtagc
      421 cttaatggtc ggcctttctt gcgcctgttt ctgtaaactc aataaaggtg gctgggatgg
      481 tgccgtcatc accttctttg ccagtacggt cgcgatgtat atccgccagc tgctggcaca
      541 acgtcatctt catccacaga tcaacttttg cctcaccgct ttcgccgcca ccaccatttc
      601 cggtttgctt ttgcaacttc ccactttcag caatacaccc accatcgcga tggccgccag
      661 cgttctgctg ctggtgccgg gttttccgtt gattaatgcc gtcgccgata tgtttaaagg
      721 ccacatcaat accggactgg cacgctgggc gatcgccagt ctgctgacac tggctacctg
      781 cgtcggcgtg gtgatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt
      841 ctgttagcgt tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg
      901 gtgttcaacg ttcccgtgcg ggcgttacgc tggtgtgcgc tgcttggatc gataggtcat
      961 ggttcccgaa tgatcttaat gaccagcggg ctgaatattg agtggtcaac ttttatggct
     1021 tctatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa
     1081 gtgtttaccg tggcggctgt tattcctatg ttccctggca tatcggctta taccgcaatg
     1141 atttcggcgg taaaaatcag ccagctaggt tacagcgaac cgttgatgat taccctgtta
     1201 accaactttc ttacagcttc atcaattgtt ggtgcgttat ccatcggtct ttccattcct
     1261 ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta agcacacgga
     1321 tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct tctcgttcat
     1381 cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg tcgttggtat
     1441 tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc
     1501 cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc tggctgaact
     1561 gctagcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg acgatcaact
     1621 ctatcaggaa ccgcaagctg ctcccgttgc tgtacccatg gggaaattcg ccatgtatcc
     1681 ggactggcaa cccgatgccg actttatccg cctggcggcg ctatggggcg tggcactaag
     1741 agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg cggaaggtaa
     1801 agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc
     1861 cagcaacggc ggactgccga aacgagatgt gaatacggtc agtgaacctg acagccaaat
     1921 tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca acgcctgcaa
     1981 aaaatgatgc ctgcccatat caaacctgct ttcaaaacgg gtgaagaact actggcctgg
     2041 cagaaagaac aaggggcgat ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa
     2101 atgcagcgca cctttaaccg ctccggtatt cgtccactgc atcagaactg ctcctttgag
     2161 aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg ccagtatgtc
     2221 gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg aaccggcaaa
     2281 aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc
     2341 atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag cggtaccagc
     2401 gaagaacaac tacttaacga tctgagcaac gttgatctgc tggtgatcga tgagatcggc
     2461 gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct
     2521 tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat gaccaagttg
     2581 ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt gatcttcaac
     2641 tgggatagct accgtagccg ggtaacgggt aaagagtatt aatccccgtt tctggcggta
     2701 tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca tcttctgtgc
     2761 tgtgccattg ccgccagcgc attaatttcc accggggtgc atgctgcgtc ctggaaagat
     2821 gcgctctcca gcgccgccag cgaacttggc aaccaaaaca gcacgacaca ggaaggcggt
     2881 tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt aagcgcagat
     2941 aacatgaaca atgccgcagg cattctgcaa tactgcgcga agcaaaagct ggcttcggta
     3001 accgatgccg aaaacatcaa gaatcaggta ctggaaaaac tgggcctgaa cagtgaagag
     3061 caaaaagaag acaccaacta tctggatggc attcagggtt tgctgaaaac caaagatggt
     3121 cagcaactca atctggataa tattggaacg actccgctgg cagaaaaggt gaaaaccaaa
     3181 gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc gaaacgccgc
     3241 gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg
     3301 ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc
     3361 ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt
     3421 gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccgtt
     3481 gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc
     3541 gggacgtaac acctggtggt ttgcagccac attaacggta ctggggctat ttgtcgtttt
     3601 aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct
     3661 ctatacctta accaacagcc tgaccggtgc tggtgtcagt aaatacatcc tgcctggtat
     3721 cggcattgtg ctggggctga cggcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg
     3781 tcgccatcat ccgcaccatt ttggttacag cctgctggca ctcttactgg cgctgggctc
     3841 agtggatgcc agcccggcat ttcgtcagat aacggaacta gtgaaatccc agtcacgcga
     3901 cggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa
     3961 actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaggc
     4021 tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca
     4081 cacgcagcag ctgccgggaa cagattacac gattgctggc atggtggctt ctcagtgcgg
     4141 cataccgctg tttgccccct ttgaaggtaa cgcctccgcc tctgtctcca gcttcttccc
     4201 gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca
     4261 gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aaatcgcacg gcttcgacca
     4321 cttattcggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc gcaacgactg
     4381 gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg
     4441 ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt
     4501 tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga atcagtcatt
     4561 cagcgcggta agttgcagcc aggagaatat cgcgacgttt atcaacaaaa tcaaagcgtc
     4621 accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga tgaacaacac
     4681 ggcgtggaaa taccttaata aacaggatcg caataacctg ttttttgtca tccgtggcga
     4741 caagccgcag caagagacgc tggcggtgaa gcgtaacacg atggataacg gcgcgacggt
     4801 gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca
     4861 gtcaatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccggatat
     4921 catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa
     4981 catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa
     5041 acgcgtggaa ccgctgccgg aaagtgaata ctcagcaccg ctgcgtttcc agctggccga
     5101 tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tacaagatgg cacaactttg
     5161 ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caagggcagc ttggcggtca
     5221 gcaaattgtc cagcatgttg acaaaacaac atggaagggc aaaacggcat ttaaagatac
     5281 ggtcatcgac atggcgcgtt acaaaggcaa tgtcgatacg ctgaagattg ttgataacga
     5341 tattcgctac aaagccgaca gtttcatctt taacgtcgcc ggtgcgccag aagaggtgaa
     5401 acagtttagc gggatttccc gtccggagtc gtggggccgc tggtccaacg cgcaactggg
     5461 cgatgaagta aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac
     5521 tgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga
     5581 acaaactctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg ataacccaac
     5641 cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat
     5701 cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag ttgtggaacg
     5761 tgaagggtaa tttctgactg atgtgtaggt cgcataaggt gcgacctaca caccttctcc
     5821 ggcctacaaa acatcattga gaaacagaaa aaaaacagcg cccaacactt ccccggacgc
     5881 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt aatcaactca
     5941 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg gtagcgatgg
     6001 gttaacatca tgtcggcagt gatttttcct tccgccatca gtcgaccgac tttagcgaaa
     6061 tattccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg gaattccgga
     6121 tcggagaact gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc gccgtgacga
     6181 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6241 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc
     6301 agtacaggta attccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6361 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6421 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6481 ctaatagcga aaggttcaat taatgctgcc gcctgcgggt caataccatc tgcgggcaaa
     6541 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6601 actgaaattt tttcacagca attggtacgc ccgcttttac acgtcgggca ttgctgacag
     6661 gcaacataag ggatcaccgc tacttgctgt ccatttttaa gatttgcaat atttttaccc
     6721 agcccaacaa tctccccaca tatttcatgg cctaaaacgc gtggataact aaaaaatggt
     6781 tgattcccac cccaggcatg aatatcggta ccacaaatgc cgactgactt aatttttatt
     6841 aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc
     6901 tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt ttgttgcctt
     6961 agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac
     7021 gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt
     7081 cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata
     7141 tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta
     7201 cagtgcgtca tattctcagc cacttacgcg aatgcggcgt cttaacgcag gttggaaacg
     7261 actatgttat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa
     7321 tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgccagt
     7381 tacgccctgg ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg
     7441 ttgtagtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc
     7501 gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg tttgaactac
     7561 gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc
     7621 gctggctgca ggcgaaaacc atgctcgaac gtcatcggct actgcgtgac aacatcggca
     7681 atagctttcg catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg
     7741 ccgacaatat cttttttgat caatctcttg agatcatctc agtcatcttt cattttcact
     7801 atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gagcatatga
     7861 ccattctcag cgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc
     7921 atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc
     7981 attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa
     8041 ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga
     8101 gcggtagtaa agtcctaaaa ctttaacctg ccgttaatat caaccttgcg taatgcacaa
     8161 ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc
     8221 ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat
     8281 ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta
     8341 ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg
     8401 attcgtgaag aattgggatt aagtgccacc gaaatcggcg ctttgctctc cgtgttttca
     8461 ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa aggcccac
//
LOCUS       DARNVM010000188         8488 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.188, whole genome shotgun sequence.
ACCESSION   DARNVM010000188 DARNVM010000000
VERSION     DARNVM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.188"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(86..379)
                     /gene="cas2"
                     /locus_tag="SI621_004134"
     CDS             complement(86..379)
                     /gene="cas2"
                     /locus_tag="SI621_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HEI4066783.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGVRLVSFLPVDNQ"
     gene            complement(376..1299)
                     /gene="cas1e"
                     /locus_tag="SI621_004135"
     CDS             complement(376..1299)
                     /gene="cas1e"
                     /locus_tag="SI621_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HEI4066784.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1296..1946)
                     /gene="cas6e"
                     /locus_tag="SI621_004136"
     CDS             complement(1296..1946)
                     /gene="cas6e"
                     /locus_tag="SI621_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HEI4066785.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1928..2674)
                     /gene="cas5e"
                     /locus_tag="SI621_004137"
     CDS             complement(1928..2674)
                     /gene="cas5e"
                     /locus_tag="SI621_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HEI4066786.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNSHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYRLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(2685..3740)
                     /gene="cas7e"
                     /locus_tag="SI621_004138"
     CDS             complement(2685..3740)
                     /gene="cas7e"
                     /locus_tag="SI621_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HEI4066787.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3755..4291)
                     /gene="casB"
                     /locus_tag="SI621_004139"
     CDS             complement(3755..4291)
                     /gene="casB"
                     /locus_tag="SI621_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HEI4066788.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(4288..5850)
                     /gene="casA"
                     /locus_tag="SI621_004140"
     CDS             complement(4288..5850)
                     /gene="casA"
                     /locus_tag="SI621_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HEI4066789.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLDHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGVQTTKFNKDHFIKHGVTEHLCPQCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LIFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICDEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5948..>8488)
                     /gene="cas3"
                     /locus_tag="SI621_004141"
     CDS             complement(5948..>8488)
                     /gene="cas3"
                     /locus_tag="SI621_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HEI4066790.1"
                     /translation="KSNCFGLTDYFRQLGFADTEQAAQFFAWLLCWHDTGKFARSFQQ
                     LYLHPQLKVPEGARKNYEKISHSTLGYWLWHHYLSEYEELLPSSSLSPRKLKRVMEMW
                     MPMTTGHHGRPPDRIDELDNFLPEDKAAARDFLLEIKVLFPLIEIPAFWDDDEGIELL
                     KQLSWYISATVVLADWTGSSTRFFPRVAQAMDIKDYWQKALVQAQNALTVFPPKAQTA
                     PFTGINTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMA
                     AGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLW
                     SGDLVGSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLL
                     GLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFAR
                     GAEGQQEAPFLEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIE
                     SAVSQGKCIAWIRNSVDDAIKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLA
                     RFGKSCSLQRSSQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDING
                     QLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQ
                     GAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAA
                     NINDYLPEKLSTRLAEESVSLWLSTCIAGVVKPYANGAHAWEMSVVRVRRSWWKKHRD
                     EFSLLEGDAFRQWCIEQRQEPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT         2032 a         2343 c         2022 g         2091 t
ORIGIN      
        1 ataactgttt ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt
       61 tccttatttt taaagaacag ataacttatt gattatcaac aggaagaaaa gaaaccaaac
      121 gcaccccatc caaatccacc ggaatacgtc tattttcacc ccaggtctga aattcaaaac
      181 ccgactcggt attggtcgcc caggccatta ccacatttcc gcaacctgcc agttgagaga
      241 tttgctgcca gatcatctcc cgaatacgtt tggacgtatc tccgacataa acaccggcac
      301 gcacttccag gagccagatc gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa
      361 caacgacgac catactcatc cgccgcgtcc ccggtgaccg ctatcgccca gcgtttcagg
      421 ttcagggatg gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc
      481 ggcaagcact tcctcgatta acggaattaa tttgcccgtt aacttactgc tacggaaaat
      541 atcacgacag gccaacctga cttctttatc tggttctgcg ggttgcctcg ctgctatttc
      601 aaatgccttt ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga
      661 aagcggtttg ccactatgga taaatccaat agcgggcgca tatcccgcgg ctaatactgc
      721 cgcttcagaa ataccgtaca gacatgatgt ggcagcactg atgcagcgat tcacaacatc
      781 gcctttttcc cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt
      841 cgccagtaat gcatacgtct ggcgaacgcg ggatccctca attccacgta gctgctccac
      901 tgaacggcga gctggcggtg gctcacgaaa acgtaattca tacattttgc gcaccacctt
      961 cagacgtaga tcttccgtta aagccagctt tgcctggtag agtaacttat cagcacgcgc
     1021 cccaccgggt tgtccggaag agtagacgcg aacacccgct tcaccgaccc agaccagtag
     1081 cgtccccacc gtggcggcca gatgcaccgc cgcgtgggaa actctcgttc ccggttcgag
     1141 cataatgcag gcgaccgatc ccaccggaat gtgcgtgcgg atcccggttt tgtcgatcag
     1201 cacgaaagcg ccgtccagta cgtcgatttg accgtactgg aggaagatca tagaggtgcg
     1261 atcttttaac gggatcggac tcagtggtac aaacgtcacg cctctgctcc gggtttgatc
     1321 agcatcagac cacagccgaa cgcgcggctt ttgccatacc cctggctcag acgttgcaga
     1381 aataacccag gatcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc
     1441 agttgccggg agttttcccg ccgcagttgc tgttgtcggt aggcatcgac cgaggtatcc
     1501 agtagtgtaa aaccgcttct ttccccctgc gctgccagcc agtccagcgc cgcctgttgt
     1561 tgatgcaacc agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc
     1621 agcaagtcgt ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa
     1681 agggattgtc cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca
     1741 ctttccgccg ggcgttcctg cgacaggaca aaaaagcgaa acgccccctg gagttcttcc
     1801 cgacgataaa gaaattgcct ttctttgccg ccaggaaaaa gatcccacag ccactgatgc
     1861 attacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag ctggccggta
     1921 tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc ccctgattga
     1981 tggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg cgacgtaata
     2041 ttttgctcgc gaccaggccg tcgtgttccc cttcccacca gcattcatcc tgaagtttcg
     2101 ggagtgagac tttcagttcg cggaaactat cctgatactg ttggtatgcg ttacgtaaga
     2161 catcagacgc gttgccttcg agcagtaacg gcgcaagtgg taacgccagc ggatgacttt
     2221 ttcgccccag ataaagcgga aaaaccggat gacgtaaacc gtcctgcaac tgttcaagcc
     2281 tgtaaggcgc atcgggggtt gttgccaccg ccaccatcca ccaggcatcg gtgtagtagt
     2341 cgcgccggga gataatcgcg ctcagaagat caggggcgct caactcttcg cgacggctga
     2401 aataacgcgc tttacgcacc tcttttggca tctggaccgt gtgataatcc cgtgcccagc
     2461 gcgggttacg gctggcgcaa accaccagtg aatagtggct gttaaacgcg tttaatcgtt
     2521 cggtatcatc acgccgaatc cctaccccgg cagccagcag ccccagcaat gctgagcgcg
     2581 aaggcagttc atgggtatga cgcacttcgc cgggggcatc gacgccccag gatgccattg
     2641 gcccatgaag ctgaaaaatc aaatattggc tcattagccg ccccttacgc gcagataaag
     2701 tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaagcttgc gcattcggtc
     2761 ttctgttcat agaccgtatt catattttcg cgaagcgttg taatacgctg caccgccaca
     2821 tctaactgcc gggtgccatt aatgggttca tagaaagccg ccgccagaga gcgtggttgt
     2881 tcagtgcctt tttctgccag cgcccaggag gcgtaggcac ggctggcaaa gctgttctgt
     2941 ttgccggttg gggagacttt aagtgcggct tccgtaaagg cgcgcaaggt ctgattagct
     3001 aacgcttcgt caccgccgag gttttcgacc agcagatctt tatcgatgca gatataggtg
     3061 tagaacagcg cagaaccgaa tccggtttca ccaagatgcc cggcaccggc atcttcagaa
     3121 gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt ttcactgaca
     3181 ccaaatgcat gcgcgacctg gcaggcggct tcaacattaa attcgggttt attcgccagc
     3241 atacgaccaa acatagcgat atctaccgcc atgcgatctt tacgtaacaa agcgagatct
     3301 tcctcttttg gcgcgcgctt ttcttcggcc agttgatggg ccagcgcttt tacggcgtca
     3361 aattctgccg ggctgatatg gactagctgt tcagtttcgg cgttagtgag cggatctttt
     3421 ggttttttgt cgtttttagc tttcccaaga taatccgcaa tttttgccgc ccattcgatg
     3481 gcttttttct cttcgatgcc tttctcaatc aggatagttg ccgcctcacg cgcaatacgc
     3541 ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga agtgcgccac
     3601 gcacgtttca gactttgtga ggaaacgcgc agacgcgttg ctccacccag taccacagtt
     3661 tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt taacaaatga
     3721 agctgaataa atgtcgtcat gagatagtcc tttattaatt ttgttcgtta tcggcgtcgc
     3781 cagcctggta atattccagc gcccagcgga tacggataaa ttctgttggc cgctgctggc
     3841 ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaata tcttctgcca
     3901 aagagataag attgacggaa ccatttaata atttgaccgc gcgacgtaac tgacgcagta
     3961 aatcatcagg cgtttttact gccgacaggc gagtaaaacg cccctccgac attaccgccg
     4021 ccagctgcgc ggcaaaaggc tgccgttcat caatagcttt aacattggcg ctaactgcgg
     4081 cgaccagcgc cagtgcggta aaacgccact cttgtgcttc aattttccac aaagtgtggg
     4141 tctgcatcag cagagaacga aacccttctg acagacaaac atcattaacc gttgtgctac
     4201 gccgcagact ggcacgattg ccgcgttttt cctgcaactc ttcgtgccat ttacgcagcg
     4261 tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc
     4321 ggctttagca ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgac
     4381 gcgtttcaaa tcaacgggct cataaggatt agtgaataca cgctcgtcaa aatcctgacg
     4441 cgcgaataac caaatttcct tttgccattt gccgagtaat tcatccgcat cctgaccttc
     4501 ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc
     4561 cacaaaactg aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc
     4621 actccgtaac agactcagaa tccgtgaagc cgtttgcgca gccagccgca gcttcggtat
     4681 ctggccttct tttttattga gcagtagcgg gaaatggtgt tcgtaccaac agcgcgcttt
     4741 catgttgtca aaatcataac caaatcccca taggcccact tttgcctgtt tcagactgct
     4801 ggcattaaag agtttcacca ccagcgcggg aagttccgta ttgttttctg atttacccgt
     4861 ttcgataagg cctaaccagt cgcgccagat taaaccgccc ggttgtggtt taacggagta
     4921 aaactcgccc ccctctttaa gtggtacacg gtaaggcgtt aagggatgct gccacatggc
     4981 ataattggca ccataatttt tggtcgtcat caggctcagg agcgcgtcgt tctgctcgtc
     5041 gcaaatatcg cagttgccga ctgtcgtggt attaaaatca atacgtatac gccgcggcat
     5101 tccccagtac gcctggagtt tattgacctg atcatgggtt accaccgcac cggccagttc
     5161 gctggtacgc gtcgggccta gccaggggaa aatcagatcg tcaaattttt tgggtagcgg
     5221 taagtcggct tcatcctgcg gcatcacgtt gagccatagt ttgcgccaca agggagtttg
     5281 ttgattgccc tgatattcct gtaattcaat cagcgtggtc atcggcccac cgccgcgtaa
     5341 gccggtgcga tagcctttgc cacctgacgg cgcatttaac tgtagagaga acaaggctag
     5401 tgcagaacac tgtgggcaca ggtgttccgt tacaccatgc ttgataaagt ggtctttatt
     5461 aaactttgtt gtttgaacgc cgggaatctc aggcagtagc gaagcgaccg gaactttatc
     5521 tcccgtgagc gcctcaaaat cctgcataaa tgaaggtgaa tctggcccga actggaaagc
     5581 atggtctaat gacagcaatg cttcccgtag cttttcagct tccagtccgt cttcccagat
     5641 atcatcccaa cgacgttgat cttttggcgc gaagctggtt tgtaatagtc ccagcaaaaa
     5701 ctgccatgcc gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc
     5761 cgccagatcg actggcgcca gtttgcctgt cgttccgtct ttataacgaa cgggcaacca
     5821 cggggttgtc agaagtgaaa acgagttcat acgcatcctc tcgtaaaaaa gaccatccct
     5881 ggtcggttgt ccatcattcc atccttttcg gtgtatcaac tattcagaga gccctgtcgc
     5941 aggcatctca accaaccttg ccagtcaatc cttccatagc cgaatacccg caactttcgt
     6001 catcggtgac taaaatcacg tttgccatct ccggctcttg ccgctgttca atgcaccact
     6061 gcctgaacgc atccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc
     6121 gacgcactct gacaacgctc atttcccatg cgtgagcacc gttggcataa ggcttcacca
     6181 caccggcaat acaggtagac agccacaggg aaacagactc ctcagccaga cgtgtcgaca
     6241 gtttttccgg gaggtaatcg ttgatattgg cggcatagcc gggcctgaag ttcagcacaa
     6301 actttttagc cattgcgcga tcgcagtaat atttgcccac ttgttcctgc tcgctgcggg
     6361 caaatccttc cggcattacc acgtcctcac cgtagactga ttcaataaga aggcgggctg
     6421 cgtgtggcat ttgaatcgcg ccttgctcac gcagtacacg ctgcgtcagc cagattcgtc
     6481 catgatcggg atagacatat gcactgttac gcatggcact gccgaaccat tcgtcacctg
     6541 gagcgtcgtc ccagacgggg gccagaatca gcaattcagg aggggaacgc tcgtcttttc
     6601 cgtcacgctt taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgcccgtt
     6661 ggatcagcaa atcaacaggg gccaggtcgg atatcatttc gtccaggtca atatccacac
     6721 tttgttcaat tacttgtgta caaacaatga cttgcgatga tctctgtaaa ctacaacttt
     6781 taccgaagcg tgccagcgtt tccgtttcaa ttcgctggcg atcgctaaaa gcaaagcggc
     6841 tatgaaacag agaaaggctg gaagcaggaa tgacgccgcg ggcaagcaac tgacgataaa
     6901 ccttaatagc gtcatcgaca gaattccgga tccaggcgat gcatttcccc tgacttactg
     6961 ccgattcgat acgcgcaata cattcttgtt cgctatgaag ccaacccacg ctcacgctac
     7021 gctcaacgtc tttgcgcgtt gctacccggt gtgaatgcac atccgatttc gtaacgtgcg
     7081 tcagccaggg gtaatcatcc ttttccagga acggagcttc ttgctggccc tctgcaccac
     7141 gcgcaaaggc agcaacgagt ttgtcgcgct gctgttgaga taacgtggca gaaagcaaaa
     7201 taacgctgtt tcctccacgc gcctgccgct caatcaatcc ttcaagaatg cacgacatgt
     7261 aagcgtcaca ggcatggatc tcatcagcca gcaggatttt gttactcagt cccagaagcc
     7321 gcagattatt atgtttaaac ggcatcaccg ccatcatcgc ctgatccagc gtgccgacgc
     7381 caatttcagc cagcaacgcc ttcttgttac tgtcggcaaa ccaggccgca catccctgac
     7441 tgaacgcttg ttcatccggt tcttctgacc cgactaaatc accggaccag agtgattcat
     7501 taaagcggtc cattaatgtg cgggcactgt gtgccagcac caggctgggg cgggattctg
     7561 gcgaatagaa agcaagccag gtttttacca gccgatcgta catggcattg gccgttgcca
     7621 ttgttggcag gccaaaaaac aaaccctgtg ctttccccgc agccatcaac ctgtgggcca
     7681 ggataagcgc cgcttctgtt ttacctgcgc cagtcacatc ctccagaata aataactgtg
     7741 gcccaggctg gctgatatcc agatccagca ccttttgctg taacggtgtc gggtgctcaa
     7801 taaaaggaaa cagcgtatta attccggtga aaggcgcggt ttgtgctttt ggaggaaaga
     7861 cggttaaggc gttttgagcc tgaactaaag ccttctgcca gtaatcttta atatccattg
     7921 cctgtgcaac gcgtggaaaa aaccgcgttg acgatcccgt ccagtctgcg agtacgacgg
     7981 ttgcagagat ataccaggaa agttgtttta aaagttcaat gccctcgtca tcatcccaga
     8041 atgcggggat ctcgatgagc ggaaacagta ccttgatttc aaggagaaaa tctcgtgcgg
     8101 cagctttgtc ttcaggcaga aaattatcca gttcatcaat acggtcaggg ggccgaccat
     8161 gatgcccggt agtcatgggc atccacatct ccatcacccg tttaagtttg cgcggagaga
     8221 gtgaagatga aggaagcaat tcttcgtatt cacttaaata atgatgccac agccagtagc
     8281 ccagcgttga atgagagatc ttttcgtaat tctttcttgc gccttccgga accttgagtt
     8341 gtgggtgcag gtaaagttgc tgaaaagagc gggcaaattt tccagtatcg tgccagcaca
     8401 atagccaggc gaaaaattgt gctgcctgct ccgtatcagc gaaacctagt tgacgaaaat
     8461 agtcagtcag cccgaagcaa ttgctttt
//
LOCUS       DARNVM010000189         8400 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.189, whole genome shotgun sequence.
ACCESSION   DARNVM010000189 DARNVM010000000
VERSION     DARNVM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.189"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..548)
                     /locus_tag="SI621_004142"
     CDS             complement(<1..548)
                     /locus_tag="SI621_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEI4066791.1"
                     /translation="MKNIATGGVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALNQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCQVDLLVLDEIGIQRETKNEQV"
     gene            complement(555..1412)
                     /locus_tag="SI621_004143"
     CDS             complement(555..1412)
                     /locus_tag="SI621_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HEI4066792.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLREFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTAPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRRVNTVSLPD
                     SAIPPGFRG"
     gene            complement(1425..1847)
                     /locus_tag="SI621_004144"
     CDS             complement(1425..1847)
                     /locus_tag="SI621_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066793.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETEAQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(1844..2098)
                     /locus_tag="SI621_004145"
     CDS             complement(1844..2098)
                     /locus_tag="SI621_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEI4066794.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            2178..2597
                     /locus_tag="SI621_004146"
     CDS             2178..2597
                     /locus_tag="SI621_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066795.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            3033..3185
                     /locus_tag="SI621_004147"
     CDS             3033..3185
                     /locus_tag="SI621_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HEI4066796.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LARNTC"
     gene            3596..3817
                     /gene="kilR"
                     /locus_tag="SI621_004148"
     CDS             3596..3817
                     /gene="kilR"
                     /locus_tag="SI621_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HEI4066797.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHNGRTYIASANNIKKRKLYIR
                     NLTTKTCITDCMIKVFLGRDGLPVKAESW"
     gene            3811..3987
                     /locus_tag="SI621_004149"
     CDS             3811..3987
                     /locus_tag="SI621_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066798.1"
                     /translation="MVMTKKIKCAYHLCDKEVKESKSIKRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            4062..4337
                     /locus_tag="SI621_004150"
     CDS             4062..4337
                     /locus_tag="SI621_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066799.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLHEGVKKMCLVHTGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            4439..7039
                     /gene="recE"
                     /locus_tag="SI621_004151"
     CDS             4439..7039
                     /gene="recE"
                     /locus_tag="SI621_004151"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HEI4066800.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGKGFVHDLTSLARDVATGVLARS
                     MDLDIYNIHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEIIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGETETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDANKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGKVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            7032..7841
                     /gene="recT"
                     /locus_tag="SI621_004152"
     CDS             7032..7841
                     /gene="recT"
                     /locus_tag="SI621_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HEI4066801.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDASVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            7898..8092
                     /gene="ralR"
                     /locus_tag="SI621_004153"
     CDS             7898..8092
                     /gene="ralR"
                     /locus_tag="SI621_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HEI4066802.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            8085..8273
                     /locus_tag="SI621_004154"
     CDS             8085..8273
                     /locus_tag="SI621_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HEI4066803.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKYKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
BASE COUNT         2442 a         1951 c         1948 g         2059 t
ORIGIN      
        1 acctgctcgt tttttgtctc gcgctgaatg ccaatttcat caagaaccag cagatccact
       61 tggcacagtt cccgcaaaaa tttttcgcct gattgcccgt cgtcatagct agcgtgtaga
      121 gcactcatga catcagccac ggtaaccaca atcactgtct ggccatcttt cagcaggcga
      181 ttcccgatag ccgccgccag atggtttttt ccggtaccag gttttccgct gaacgcaaaa
      241 tttgtacacc cggtcatcag ttcatcagcg atagatttcg cctggttcaa cgcatatcgc
      301 tgaccgtcgt tctgcacctg gtaattcgca aacgagcatt tgcggtgcaa tggctggatg
      361 ccagagcgat tcagaatttt ttccacccgc aactgacgat tctgacggtt gatctcctca
      421 caacgtttct ggccttcgga aagttgccac tcacgccact ccgctaccgt cttgaatggc
      481 gcggttacat gtgacggggc cagtctgcgg atacgttcaa gaacgccgcc tgtcgcaata
      541 tttttcatgg tcagttaccc cctgaagcct ggcgggatcg cactgtccgg taacgaaacg
      601 gtgttaaccc gtcggagtaa cgtctcaggt cgaacacctt tcggcgcgaa caagccctgg
      661 tattcgttgg cgatgctgtg tcgaatcacc tgctcaggtg aaaaaccctg ctggcggaat
      721 ttttccagct cccgtatcgc cccgttagcg ccctgctccg ttcgaatcgg tttacgcaat
      781 gcctggcgaa attcaaccca ctcacgccaa agcgagacag aaatccagtt cggcaaagca
      841 atatccagag ggtcaaactt tttgacacct cgattccccc gggggggatt taggggggga
      901 gctgttttta gatctttatc tgtatcttta ttagttgcct ttgtgttgac atcatgttca
      961 aacaccactt caacatctgt ttgaacacct gttaaatttc tctcttgttt tgtttgaaca
     1021 tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc tgattttttg
     1081 gttaattttt ccctgactga tgccagatct tcctcaatcc gaagatgtac ccattcctcg
     1141 ccgttatcgc aaaaaaactc ccgcaaggat ggttcaacat cagcccatcg ctcgttagtc
     1201 agacgggcaa tttttgccag cctgtttttg ggtattggct ttcctgtttg ccagtaattg
     1261 aacatcagca acaaatacgc gccatgctcc tctgctgaca aatgcatggt gtcagccagg
     1321 taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc gtatgatgct
     1381 gcccggtggc ttagaataag cacaaacagc atggaaactt ttgcttaatg aacaatgaca
     1441 gaatcgtcgg aagacccgcc gccgctgaaa tgcgctttcc ggtaaacggc ctggactgca
     1501 tcatcatgcg catcaattgc cgtacttaac gcttcctgcg ccgccagtaa tgcacggcgt
     1561 tccagggtat cgaagatgca gagtcggtga cgcagctcgc gcggaaggat tgccagaatt
     1621 gcagggatca gtttctgaat tttttccctt tgcgcttccg tttcaccttt taaccaacgg
     1681 tgatagatgt tctgctgatt attccagtcc ttgcctggta ccaggggcaa ttcgccgccc
     1741 ccctggcgca gatattcttc agtaatggca ttggctaccc atgcctgccc tttttcggct
     1801 gccagggcta acaacactga ctcgatgtgc tcatgcttga ttttcatgaa tcaactccca
     1861 tcagcttttt cgtagtagtt ttatttctgc caatagttaa aatcacatcg gcagaaaata
     1921 atccgtttga tgcatgagcg attttttcag cgtaatttgt ttcgccggta tattctgtgc
     1981 gaggcaattt tccgttatcc atccatttgt agattgctct ttggctgaca ccacaaacgt
     2041 cggccacaac agaaacgcga acagttttga ttacatcttc aagtgttttc tggttcatat
     2101 caccctcaca atgtgaactt tgagtacatg ctataacaga actgacagta cattcaagag
     2161 cgaatatcat tgaacttatg gttcatgaag ataaagcgcg taaagagttc gccagtaggc
     2221 ttgcgctagc ctgtgaaaac gctggttatg aacaacatgg aaggcaggca gaaattgccc
     2281 gtcgaatgaa attaacacca aaagcggtta gcaaatggtt taatggcgaa acaattcctc
     2341 gccgagagaa attaagggaa ttagcaacac tcattggaac aacaccaacc tatcttttgg
     2401 gagaggatac agaagaaagt ggacaggtac gtttctatca ggagttaaat ccaagacaaa
     2461 aaatcatcat tgaccttctg gacgagctcc ctgacagtga gacagatgaa cttttaaaaa
     2521 ctcttgagga gaaaaaacag aagtacaatg caatttacga agaattagca cgaaagaaaa
     2581 aacaaaaagc ctcttaaacc agcataaatc cggtagcgtc cccctccggg tttgtgcttc
     2641 actttatccc atctcatttt tttacacaca agatgtactt aaagtacttt acatcaatga
     2701 acacaaagta cattatatac ctaccaaccc accccgcccc acagaacgca gggcaatact
     2761 tcgagttacc aggcagtggt caggggttaa gtagccagcc cgaggcgtat gaacatgacg
     2821 gcagggttca gattttgcag tgcagcaggt ttcagttccg ccacccggcg ttaaggggag
     2881 agataagatg gtgcattacg aagtagttca gtatttgatg gatagttgcg gtatcactta
     2941 caaccaggct gtgcaggctt tacgcagcaa cgactgggat ttctggcagg cagaagtcgc
     3001 tatacgcagc aacaagatgt gagattcgca aaatgcaaaa aatcgacctc ggcaacaacg
     3061 aatccctggt gtgcggcgtg ttccccaacc aggatggaac gttcactgcc atgacttata
     3121 ccaaaagcaa gacattcaaa actgaaacgg gcgcacgtcg ctggcttgcc agaaatactt
     3181 gctaatccat tatttggatt aattcaatat tctcgctgta ggggtatagc agaaaccacc
     3241 gaagcccgga ggtggtgaaa taaaattggg cgcaacacga aggcgcattt ccgatatcca
     3301 taaagagtcg gtcttgtctg ttaaatttaa atggtgggag tgcgcctccg gttgtaaata
     3361 acgacattgc tgtgtgtagt cttggcggca tcagtttttt cttgaagttc ggctgatgtc
     3421 cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac gcggaacagt
     3481 taaaagcatc agtgttatgg gtggattatc cggcgttaat tgttaactgg ttaacgtcac
     3541 ctggaggcac caggcaccgc atcaacaaag ttcatttgtg aaaatggaga taattatgat
     3601 tgctcatcac ttcggaactg atgaaatacc tcgtcagtgt gtgactcctg gtgattatat
     3661 tcttcataac ggccggacat atattgcctc ggcaaacaat attaaaaagc gaaaacttta
     3721 tattcgtaac ctgaccacaa aaacatgcat tactgactgc atgattaaag tcttcctcgg
     3781 tcgtgatggt ttacctgtaa aggcggagtc atggtgatga ctaagaaaat aaaatgtgct
     3841 taccatcttt gcgataaaga agttaaagaa agcaaaagca ttaaaagacc acttcatttc
     3901 atgcgtggag ttatcccaac gacggaaatg aaaaaatatt gtagtgaaat atgtgccgaa
     3961 aaagaccaga tggcacacga actttaatta actgactatg cgaaactgaa tttatgccag
     4021 caatggcagg gattcgctca accttaatta aggagaaaaa catgattacc agttatgaag
     4081 ccactgttgt aactactgat gacattgttc acgaagttaa tctggaagga aagcgtattg
     4141 gctacgtgat taaaacagaa aataaagaaa ccccattcac tgtggttgat atcgacggtc
     4201 catcaggcaa cgttaaaaca cttcacgaag gtgtcaaaaa aatgtgcctg gtgcataccg
     4261 gaaagaatct gcccgcagaa aaaaaagccg aatttctggc aactctaatt gcaatgaaat
     4321 taaaaggtga aatctgaaag aaatagcctg cgtatggcgc aggctatgaa cagtgtgtat
     4381 ccggcaagat cattcactga acaaaacgaa ttttaatctg agttgaggtt aaaaaacaat
     4441 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
     4501 cgtcctgtgg gcaagcgacg attttgaatc gacctgtgcc actctggact acctgatcgt
     4561 taagtcaggt aaaaaactga gcagctattt taaagctgtt gccacgaatt ttcctgtcgt
     4621 taatgacctg cccgctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
     4681 caaagactcc atgacatggg aactaaaacc gggagcagca ccagacaacg ctcactatca
     4741 aggcaatacc aacgtcaacg gcgaagacat gactgagatt gaggagaata tgctactccc
     4801 aatttctggc caggaactgc ccattcgttg gcttgctcaa cacggcagcg aaaaaccggt
     4861 aacgcacgtt tcacgcgacg gactccaggc attacacatt gctcgggctg aagaactacc
     4921 ggctgttact gccctggctg tttcccacaa aaccagcctg ctcgacccgc tggaaattcg
     4981 cgatctccac aaactggttc gtgacactga caaagttttc cctaatcctg gtaattcaaa
     5041 cctgggactg ataactgctt ttttcgaagc atacctgaac gctgactaca ccgatcgagg
     5101 actgctgaca aaagagtgga tgaagggtaa tcgtgtttca cacatcactc gcacggcttc
     5161 cggtgctaat gctggcggcg gaaacctcac cgatcgcggc aaaggtttcg tacacgatct
     5221 gacgtcactg gcgcgcgacg tagccactgg cgtactggcc cgttcaatgg atctggacat
     5281 ctataacatt catccggcac acgctaaacg cattgaggaa attatcgctg aaaataaacc
     5341 gcccttttct gttttccgcg acaaattcat caccatgcct ggcgggctgg attattcccg
     5401 cgccatcgtg gttgcttccg taaaagaagc accaattggg atcgagatca tccccgcgca
     5461 cgtcactgaa tatctgaaca aagtactgac tgaaaccgat catgccaacc ctgatccgga
     5521 aatcgtggat attgcctgcg gtcgctcctc tgccccgatg ccgcagcgag taacagaaga
     5581 aggaaaacag gatgatgaag aaaaaccgca accatctgga acaacggcag ttgaacaggg
     5641 agagactgaa acaatggaac cggacgcaac tgaacatcat caggacacgc agccgctgga
     5701 tgctcagtca caggtaaatt ctgttgatgc gaaatatcag gaactgcggg cagaactcca
     5761 tgaagcccgg aaaaacattc catcaaaaaa tcctgtcgat gccaataaat tgcttgctgc
     5821 atcacgtggt gaatttgttg acggaattag cgacccgaac gatccgaaat gggtaaaggg
     5881 gatccagact cgcgattctg tgtaccagaa ccagccagaa acggaaaaaa ccagcccgga
     5941 tatgaatcaa cctgagccag tagtgcaaca ggaaccggaa atagcctgca atgcctgcgg
     6001 ccagactggc ggggataact gccctgactg tggtgcggtg atgggcgacg caacatacca
     6061 ggaaacattc gatgaagaga gtcaggttga agctaaggaa aatgatccgg aggaaatgga
     6121 aggcgctgaa catccgcaca atgagaatgc tggcagcgat ccgcatcgcg attgcagtga
     6181 tgaaactggc aaagtcgcag atcccgtaat cgtagaagac atagagccag gtatttatta
     6241 cggaatttcg aatgagaatt accacgcggg tcccggtgtc agtaagtctc agctcgatga
     6301 cattgctgat actccggcat tgtatttgtg gcgtaaaaat gcccccgtgg acaccacaaa
     6361 gacaaaaacg ctcgatttag gaaccgcttt ccactgccgg gtacttgaac cggaagaatt
     6421 cagtaaccgc tttatcgtag cacctgaatt taaccgccgt acaaacgccg gaaaagaaga
     6481 ggagaaagcg tttctgatgg aatgcgcaag cacaggaaaa acggttatca ctgcggaaga
     6541 aggccggaaa attgaactca tgtatcaaag cgttatggct ttgccgctgg ggcaatggct
     6601 tgttgaaagc gccggacacg ctgaatcatc aatttactgg gaagatcctg aaacaggaat
     6661 tttgtgtcgg tgccgtccgg acaaaattat ccctgaattt cactggatca tggacgtgaa
     6721 aactacggcg gatattcaac gattcaaaac cgcttattac gactaccgct atcacgttca
     6781 ggatgcattc tacagtgacg gttatgaagc acagtttgga gtgcagccaa ctttcgtttt
     6841 tctggttgcc agcacaacta ttgaatgcgg acgttatccg gttgaaattt tcatgatggg
     6901 cgaagaagca aaactggcag gtcagctgga atatcaccgc aatctgcgaa ccctggctga
     6961 ctgcctcaat accgatgaat ggccagctat taagacgtta tcactgcccc gctgggctaa
     7021 ggaatatgca aatgactaag caaccaccaa tcgcaaaagc cgatctgcaa aaaactcagg
     7081 gaaaccgtgc accagcagca attaaaaata acgacgtgat tagttttatt aaccagccat
     7141 caatgaaaga gcaactggca gcagctcttc cacggcatat gacggctgaa cgtatgatcc
     7201 gtatcgccac cacagaaatt cgtaaagttc cggcgttagg aaactgtgac actatgagtt
     7261 ttgtcagtgc aatcgtacag tgttcacagc tcggacttga gcccggtagc gccctcggtc
     7321 atgcatattt actgcctttt ggtaataaaa acgaaaagag cggtaaaaaa aacgttcagc
     7381 taatcattgg ctatcgcggc atgattgatc tggctcgccg ttcaggtcaa atcgccagcc
     7441 tgtcagcccg tgttgtccgt gaaggtgacg agtttaattt cgaatttggc cttgatgaaa
     7501 agttaataca ccgcccagga gaaaacgaag atgcctcggt tacccacgtc tatgctgtcg
     7561 caagactgaa agacggaggt actcagtttg aagttatgac gcgcaaacag attgagctgg
     7621 tgcgcagcca gagtaaagct ggtaataacg ggccgtgggt aactcactgg gaagaaatgg
     7681 caaaaaaaac ggctattcgt cgcctgttca aatatctgcc cgtatcaatt gagatccagc
     7741 gtgcagtatc aatggatgaa aaggaaccac tgacaatcga tcctgcagat tcctctgtat
     7801 taaccgggga atacagtgta atcgataatt cagaggaata attcagcctg gcggtgtaat
     7861 gcaccgccaa cttgaaatat tttttatgag aaaaattatg agatatgaca atgttaaacc
     7921 atgtccattt tgtggttgtc catcagtaac ggtgaaagcc atttcaggat attaccgagc
     7981 gaagtgtaac ggatgcgaat cccgaaccgg ttatggtgga agtgaaaaag aagcactcga
     8041 aagatggaat aaacgaacca ctggaaataa taatggaggt gttcatgtat aaaattaccg
     8101 ctactattga aaaggaaggt ggcactccta ctaactggac aagatattca aaatataaac
     8161 taactaaatc agaatgcgaa aaaatgctct caggtaaaaa agaagcaggc gtttccagag
     8221 agcagaaagt aaaactgata aattttaatt gcgagaaact tcagtcctcg tgaattgcat
     8281 tgtattcaaa ttaaaacttc atagctgatt attaataatc aacatcgggc gtcaatctaa
     8341 gtctaacatt ggcgcctgcc agaggtgatg cgatggcaca agtaatcttt aatgaagagt
//
LOCUS       DARNVM010000190         8224 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.190, whole genome shotgun sequence.
ACCESSION   DARNVM010000190 DARNVM010000000
VERSION     DARNVM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.190"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            12..1127
                     /locus_tag="SI621_004155"
     CDS             12..1127
                     /locus_tag="SI621_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEI4066804.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSYRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1721..2572
                     /gene="hchA"
                     /locus_tag="SI621_004156"
     CDS             1721..2572
                     /gene="hchA"
                     /locus_tag="SI621_004156"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HEI4066805.1"
                     /translation="MTVQKSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESEDVAAALQWAIENDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2679..4037)
                     /gene="hprS"
                     /locus_tag="SI621_004157"
     CDS             complement(2679..4037)
                     /gene="hprS"
                     /locus_tag="SI621_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HEI4066806.1"
                     /translation="MKRLSITVRLTLLFILLLFVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSNGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNNEVENLLDYLEYLSDEKEICF
                     KVECNQHIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4037..4708)
                     /gene="hprR"
                     /locus_tag="SI621_004158"
     CDS             complement(4037..4708)
                     /gene="hprR"
                     /locus_tag="SI621_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HEI4066807.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSSLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLISTIRGMGYSF
                     VAVKK"
     gene            4841..5254
                     /gene="hiuH"
                     /locus_tag="SI621_004159"
     CDS             4841..5254
                     /gene="hiuH"
                     /locus_tag="SI621_004159"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEI4066808.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5363..6367
                     /gene="msrP"
                     /locus_tag="SI621_004160"
     CDS             5363..6367
                     /gene="msrP"
                     /locus_tag="SI621_004160"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEI4066809.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILNVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            6368..7003
                     /gene="msrQ"
                     /locus_tag="SI621_004161"
     CDS             6368..7003
                     /gene="msrQ"
                     /locus_tag="SI621_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEI4066810.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLAWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNQLRKQVHNKLSL"
     gene            7260..7910
                     /gene="zinT"
                     /locus_tag="SI621_004162"
     CDS             7260..7910
                     /gene="zinT"
                     /locus_tag="SI621_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HEI4066811.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRHNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDTQQSLLNEMENWPTYYPYQLSSKEVVEEMMSH"
BASE COUNT         2336 a         1880 c         1740 g         2268 t
ORIGIN      
        1 gggaatacat aatgaaaaga aaagttctgg caatgctggt cccggcgtta ttagttgctg
       61 gcgcagcaaa tgcggctgaa atttataata aagatggcaa taaagttgat ttctacggca
      121 agatggttgg cgaacgcatc tggtcaaata ctgatgataa taacagcgaa aacgaagata
      181 cctcctatgc tcgtttcggt gttaaaggtg aaacccaaat caccagcgaa ctgactggtt
      241 tcggtcagtt tgaatacaac ctcgacgcca gcaaacctga aggtgaaaac caggaaaaaa
      301 cccgtttaac cttcgccggt ttgaaatata acgagttagg atcatttgac tatggccgta
      361 actacggtgt tgcttatgat gccgcagctt ataccgatat gttagttgaa tggggtggcg
      421 attcctgggc ttccgctgac aacttcatga atggtcgtac caacggtgtt gcaacctacc
      481 gtaactatga tttctttggc ctggttgatg gtcttgattt tgcgattcag taccaaggca
      541 aaaacagcta ccgtagcact aagaaacaaa atggtgacgg ctatgcgttg tctgtagact
      601 ataacatcaa tggttttggt atcgttggtg catatagcaa atctgatcgc accaacgatc
      661 aagttgccga tggaaacggt tcaaatgctg aactgtggtc attggctgca aagtatgacg
      721 cgaacaatgt ctacgcagct gtaatgtacg gtgaaactcg taatatgact ccgggttcta
      781 ttgatacagg tgttgccgat cgagaaggca atacaataat gcgtgatcaa cttatcaatg
      841 aaacccagaa ctttgaagcg gttgttcagt atcaattcga ctttggtctg cgtccgtcct
      901 taggttatgt gtattctaaa ggtaaagata tcaaaggtgt acctggacat agatatgttg
      961 acgctgaccg tgtgaactac attgaagttg gtacctggta ctacttcaat aagaacatga
     1021 acgtttatac tgcatacaaa ttcaacatgc tggataaaga tgacgccgcc attactggcg
     1081 cagcagctga tgaccagttt gcagttggta tcgtctacca gttctaatca gccaacgcgc
     1141 cgcggcgtgt tactgtaaaa cttaacacta gcctgagttc gtccctcatc gaactcaggc
     1201 ttttttattg gcatttcatc tcgatctgcg gcacacgcaa ttgccaaagc atatcgatac
     1261 agcaccttac cccgcccccc cctggctcaa taaaatctac cagcgtctct tccagccaac
     1321 acccaaatgc atttctttat aaatttaatc tgtaacaaaa ccgcttccca tatagaaata
     1381 tttgcaaaaa tagtgcatta aaaaagcttt ttccctacca atccgggata taaatattca
     1441 tcagcaccat aatattccac ataactaaat ctctccctgc cgccccgtac ctctgataat
     1501 ggtctaaaat cattgaggcc acttgcgacg gcgttgctca ataaggaaaa tttggcgtga
     1561 ataatattca acaccagtgc agcatcatca ttacatactt acgcccgccg actaccgctt
     1621 tcgatatatt gactcaattt tccagcctgg ttaaaaatag cggccagctc agtcgcaaat
     1681 atagtgacta ccctaactaa gcaacaataa ggaatacact atgactgttc aaaaaagtaa
     1741 aaatccacag gtcgatattg ctgaagataa tgcattcttc ccttcagaat attcgcttag
     1801 ccaatatacg agtcccgtct ctgatcttga tggcgtggac tatccaaaac cgtatcgcgg
     1861 taaacataaa attctggtga tcgccgctga tgaacgctat ttgccgactg ataacggcaa
     1921 actcttctca acgggcaatc atccgattga aacgttgctg ccgttgtatc atctccatgc
     1981 tgcaggtttc gaattcgaag tggcgaccat ttccggtctg atgaccaagt ttgaatactg
     2041 ggctatgccg cacaaagatg aaaaagtaat gccattcttt gagcagcata aatcgttgtt
     2101 ccgcaatccg aagaaattag cggatgttgt cgccagcctg aacgcagaca gcgaatatgc
     2161 agcgatcttt gttcccggtg gtcatggtgc actcatcggt ttgccagaaa gtgaagacgt
     2221 agccgccgct ctgcagtggg ctattgaaaa tgaccgtttt gttatctccc tatgccatgg
     2281 tccggctgct tttctggcgc ttcgccacgg cgataaccca ctgaacggtt attccatttg
     2341 tgcattcccg gacgccgcag acaaacaaac gccagagata ggctatatgc cgggtcatct
     2401 cacctggtac ttcggcgaag agctgaagaa aatgggcatg aacatcatta atgacgacat
     2461 caccgggcga gtacataagg accgtaaagt tctcaccggc gacagtcctt tcgcagcgaa
     2521 tgcgttgggt aaactggcgg cacaggaaat gctggcagct tacgcgggtt aatccctgtt
     2581 acgtttgaat gtaagcgcat aatcaatcgc agccgcgtta gtactcacga cgtggctgcg
     2641 tttttcacgg ttaatttatg gcgtactgaa gccctatgtt aatttctttg cggtaacgta
     2701 atccggaaca cattatgctt actgagatag tgatacgtag cacttccccc atgtaattcg
     2761 gcaatcgctt tgactaaaga aagaccaagt ccctgaccta cggaatgacg cgaattatct
     2821 ccccgccaaa atctacggaa gagtttttca ggctcatgaa ttttcgttcc aggactggcg
     2881 atatcaatat taagagaacc gttggtatca agaaaactgg ttatctgaat acgcgatttt
     2941 tctggcgaat atcgaatagc gttaacaata agattcgata acattcgttg tagtaaaatt
     3001 ttatccgcaa agatgtgctg attgcactcg accttaaagc aaatctcttt ctcgtctgaa
     3061 aggtattcaa gatagtccaa caaattttcg acttcattat tgagagaaag cgagtccagt
     3121 ttcaccaaaa cattattttt atctgccctg gcaagaaaca gtatgttctc tgttaatcgc
     3181 gaaatatttt ccagctcttc aatgtttccg gcaattgttt tttgatattc agcgatactt
     3241 ctggtttgac tgagcgtaac ctgattctga cccagtaatg cattaattgg cgttctaagt
     3301 tcatgagcga gatcgtcagc aaactgactt agacgttcaa aatctttgac taaagcttgg
     3361 tgcattttat tcaacgcctg cccaagaggt tttagttctc tcggtaatgc gctaacctca
     3421 acaggctctc ggctatcgtt ataattcagc gcttccgtta caccactcaa ctttttgatc
     3481 tctcgtaacc ccgttctgat taacaacgga cttaatactg agcacagtat aatggcgaca
     3541 atgcaaatta taatgctatt aattttatac tgttcaagca tgttatgtct ggctgaagcc
     3601 aatttagcca cagtaaccgt taatgatggc gaaacttcat caatattaat tcgtaaagca
     3661 tctatctctg tgtcattaat aatgcttctg taaatgccag ctgcgctgat tgtctcacta
     3721 gcaggtatgt tatttaacat gccattactg acatttgtcc ggttaacaat tttattgatg
     3781 ccatcaccat gaatgatcaa gatatcctga ctaacatcca tcatccggtt aaagtacaca
     3841 ggtaacgtat ctggatttac cccatcaatt aacaactgct tgatctgcgc tgtccggtta
     3901 atgagtgttg tatcatcgcg ccatttcaac tcacttgcca ggccattata gagagtccag
     3961 acaattccag cgccagcaac aaacagcagc aatataaaaa gcaaggttaa acggacggtt
     4021 atagataatc ttttcattat ttttttaccg ctacgaatga atagcccatc ccccggattg
     4081 tggagattag cttttcagga aaaggatcat caacttttgc gcggagcctg cgaatggcga
     4141 cgtccaccgt attggtatca ctatcaaagt tgattcccca aatttcactc gcaataaccg
     4201 ttctggggat aatctcgcca gctctggagg ccagtagcca aagtaactga aactccttgc
     4261 gcgtcagtgt aatactgata ttgtccctgc tcacactttg actgacagag tccattctta
     4321 agccgctgat ttctaatgat gaattcagag cgtgatgttg ccttaattgt gcccgaaccc
     4381 ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata atcatttgcc ccactgtcca
     4441 gccctctgac tctgtcatcg acagaatccc ttgcagtaag gcaaataaca ggggtttgct
     4501 ttgctgttct taacgtttgt aagatctgcc agccatccat acccggaagc ataatatcca
     4561 gaatgatcaa tgcataatca tccttcagcg caagataaag cccatctctg ccatctgaaa
     4621 cggcatcaat gacataaccc gcttcggaaa gcccctgcgt tacccattcc tgggtccttt
     4681 gattatcttc aataagtaga atcttcatga aaataatatg ccataaatca tatgttatag
     4741 aaacagcctg gttcattaca aaattgtaat gttgctgtaa ggttaccctg gacgtttttt
     4801 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
     4861 actctccgta gtaacggctg cattttcatt accttctttg gtctatgctg cacaacaaaa
     4921 cattcttagc gtgcacattt tgaaccagca aacaggaaaa cccgctgccg acgtgacagt
     4981 cactcttgaa aagaaagcgg ataacggctg gttacaactt aataccgcca aaacagataa
     5041 ggatggacga attaaggcac tgtggcccga gcaaactgcg actactggcg attaccgtgt
     5101 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
     5161 ccccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tgttactcag
     5221 ccaatatggg tattcaacct atcgtggcag ttaatgcgta aatatgtaaa agctgatctc
     5281 tgctactgtt agggaggtga ctccctgaca gattaacagt aaacggctct tgttggctaa
     5341 cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac
     5401 ggccgagtcg gtattcttta tgaagcgtcg gcaggtgtta aaagcactgg gcatcagcgc
     5461 ggctgcactt tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
     5521 cgatcgccca cccgcccccg ccggaaaagc gctggagttc agcaagcctg cagcctggca
     5581 aaataacctg ccactgacgc cagcagataa agtttccggt tataacaact tctatgaatt
     5641 cgggttggat aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac
     5701 actgaaaatc agcggcgaag tggcaaaacc attgaccctc gatcacgatg atttaacccg
     5761 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtagaagcat ggtcgatggt
     5821 ggtgccgtgg attggttttc cgctgcacaa attgctggcg cttgccgaac ccaccagcaa
     5881 tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa cagatgccag gccagcagga
     5941 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     6001 aatgcatccg ctcacactga tgaccgtagg tgtttatggc aaggcgttac caccgcaaaa
     6061 tggcgcgccg gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     6121 agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     6181 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     6241 taccgaacga tttattggtt caggcggcat cctcaatgta cagcgccagc caacgctact
     6301 gtttaatggt tacgccgagc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     6361 tttctaaatg cgtctgacag caaaacaggt gacatggctg aaagtttgcc tgcatcttgc
     6421 cggattgttg ccgtttctct ggctggcctg ggcgatcaat cacggtggac tgggtgccga
     6481 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgctggcgac
     6541 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     6601 cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct
     6661 ggagctgggc gtgaataatc tggcgttatt aggtaaggag ttaattaccc ggccttattt
     6721 aacgttaggt attatcagct gggtaatttt gcttgcgtta gcgttcactt caacccaggc
     6781 gatgcagcga aaactcggca aacactggca acagttgcat aacttcgtct atcttgtcgc
     6841 gatcctggcc ccaatacatt atttgtggtc agtgaagatt atttcaccgc agccactcat
     6901 ctacgctggg ctggcggtac tgcttttagc cttacggtat aagaagttgc tttccctttt
     6961 caaccagtta cgcaagcagg ttcacaacaa attatctcta taacttcata ttgccaacaa
     7021 agtacgcgtt atcccggccg gatgcggcgc gagcgcccta tccggcccac acagatcgca
     7081 tcaagttatg tttcgtttgt catcaacctt tcatcaccag aataatttat tttatttcaa
     7141 taagatagga aaatacacct gagaaaacta tgctctcgtt tcctaagagt tattgcattt
     7201 cgccatatgt tacaatataa cattacacat catgtacatt aactctggag gaaactgttt
     7261 tggcgattcg tcttcataaa ctggctgttg ctttaggcgt ctttattgtt agcgctcctg
     7321 ccttttcgca tggtcatcac tcacacggca aacccttaac agaggtcgaa caaaaagctg
     7381 ctaatggtgt ttttgatgat accaatgtac aaaaccgaac gctcagtgac tgggatggag
     7441 tctggcaatc cgtttatcct ttactgcaaa gtggcaaact tgaccccgtc tttcagaaga
     7501 aagcggatgc agataaaacc aaaacatttg ctgaaattaa agattattat cgcaaaggtt
     7561 atgtgacaga tatcgagatg attggcattg aggacggcat tgttgaattc catagacata
     7621 atgaaacaac atcctgtaaa tatgattacg atggatacaa aatactcacc tataaatcag
     7681 gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc tgaaagcaaa gcccctaaat
     7741 atattcaatt tagcgatcat ataattgcac cacgaaaatc atctcatttt cacattttta
     7801 tgggtaatga tacccaacag tctttattga atgaaatgga aaactggcca acgtattatc
     7861 catatcagtt gagtagcaaa gaagtggtcg aggagatgat gtctcattga tttttttgcg
     7921 ctgacttcaa ttatttaaca gaacagtgcc tggtagtaac gacaataatt taccaagaca
     7981 aataaataag acaaactcag acacgctcat gcttgagacg tgtctttttt attatgtctg
     8041 tagaaaaaaa ctgagaaaac ggcgaacaat aaatacacaa ctcaccaata cagactggct
     8101 gcgccaatgt ctcccggagc aagtcatctg tcatggtaaa gctaaccaga aatataggtt
     8161 aattttggta tttaaacctt ttttacttgt tagtatgata caaaggcttt caaaaaagct
     8221 ggga
//
LOCUS       DARNVM010000191         8084 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.191, whole genome shotgun sequence.
ACCESSION   DARNVM010000191 DARNVM010000000
VERSION     DARNVM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.191"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..213)
                     /locus_tag="SI621_004163"
     CDS             complement(<1..213)
                     /locus_tag="SI621_004163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066812.1"
                     /translation="MEIVIYITISILILAIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQ"
     gene            complement(328..537)
                     /locus_tag="SI621_004164"
     CDS             complement(328..537)
                     /locus_tag="SI621_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066813.1"
                     /translation="MARRELELREIPYIKNSLHANYSYKSISIGSKQGWLISAKLKVP
                     ETFEPDMIFIEISDPEGFINIPDVL"
     gene            complement(576..5438)
                     /locus_tag="SI621_004165"
     CDS             complement(576..5438)
                     /locus_tag="SI621_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEI4066814.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPNDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREAAPDGSTTMYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERTGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEEHDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGWHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGL
                     REKFMMGLIN"
     gene            complement(5458..5919)
                     /locus_tag="SI621_004166"
     CDS             complement(5458..5919)
                     /locus_tag="SI621_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HEI4066815.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5947..7848)
                     /locus_tag="SI621_004167"
     CDS             complement(5947..7848)
                     /locus_tag="SI621_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEI4066816.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1587 a         2384 c         2128 g         1985 t
ORIGIN      
        1 ctggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggcagc tcagggcatc
       61 acggtcgaac agcaccaggt aatcatcgcg gcggcgcagg atgtactggg cgtgatacgg
      121 gttactggtt tcgatattgg ctaacatccg ctgttttatt aatgcgatag ctaaaatcag
      181 gatggaaata gtgatgtata tcactatttc catgatcttg ccttgcgttc atccaggcag
      241 gaatatccct gttagcagcc tctgtgcgat gtacgccagc ggtctgtaaa gaaaccgatt
      301 accttataac aaaacctcta acgtagttta taatacatca ggaatattaa taaaaccttc
      361 tgggtcagat atctcaataa atatcatatc aggctcaaaa gtttcaggga cttttaattt
      421 cgctgaaatt aaccaacctt gcttagaacc aatactaata gatttatagc tgtaattggc
      481 atgtagacta tttttaatat aaggaatttc ccttaactct aactcacgcc gagccaagtc
      541 aagagccatt tcgatagtta ttttgttatg ttttatcaat ttattagtcc catcatgaat
      601 ttttctctca atccctcaac atattttatg gcatgagcta tttccgcctc attgaataaa
      661 tttttacttt ttaatatttc atttagaacg tgttcctccc tagataaacg tcctagtgct
      721 tcgtatgctt ctcgtccaat tttcgcaaat tgcttagcat gaatcacttc atgatacacc
      781 tcatataagg tagcattttt cctaagataa atattgcgaa ggccataatc aaaagctgcc
      841 ctagcatttg gtggcaaata tttatctgct ttccttatta ctttaattcc tagcttctcc
      901 atttctttag caatattgtt tatttgacta cgtgataaac gaaccccatc caggaaaccc
      961 gtatgcccac taacacgacg accatcgcca atatagtcca tcaaactgct gattttagaa
     1021 cttgcagcca atcccagcgg atcaacccac ccacacggat ttggcacata ccggtaagtc
     1081 tgctcacccc ccagcaaccc cagcggatca ctcaccaggt acatcccggt ttccggctcg
     1141 taataccgga accggttata gcacagcccg gattctgcat cctgccactg tcccgcatac
     1201 agcaggccgg ggtcggcttc cgggtccagt tccccgcgcg ggtccgggta gccggtgtct
     1261 gcgggcaggc tgagtgccag cccccacagg ctggtctgtc ccggtcgcca gacggtttta
     1321 ccttcactgt taaagagcat cagcggacgg cccgtcaggt cgctcaccgc atgattcgtc
     1381 cgtgtcaccc actgcggggc caccgacgga tgcgcctgcc gtacccggct gaactgctgg
     1441 cttatcagct caaagccgtt aaacaccagg tgccgtacgc tgtacagttt atcatcgcgg
     1501 tattcccgga tttccgcaat actgtcgccg tcccacagat acgtgaagcg gattttcttc
     1561 cggtcgcagc gtttctctgt tcgtctgccg ctggcgtcgt ggcggtattc ccactgctca
     1621 ccctgtgcgc tgcaataccc ggtcagctgg tcacggctgt cccaccggaa ccgctctgtt
     1681 tccgggcgat agccgtcacg gtgtctggtg cggctgacca tccggcctgc ggcgtcatag
     1741 tcatactgcg tgtttccggc ctgtttcagc cggtggcctc cggcatactg gtcagtctct
     1801 tcacttatcc ggtgccagcc cgcagactgc gccttcaggt agccgcagct gtcatagcca
     1861 taggactcat cgccttcgcc atagcccagc ccggtgcctg aggctgtcac cgccgtgacc
     1921 tggccgttcc ggtccagccg gtattcccgc cgtgtttccc gtccgtaatc ttcccggctg
     1981 gtgacgcctg acacattacc cgcgctgtcg taacggtact cccgtaccag tgaggggagc
     2041 agcccgcccc atgtggcttc tgccgggaaa agtccggcgc gctgactgac aggctgaccg
     2101 gctgcattcc agccctgttc acaggcaaaa ccccggttac tgtgccggag cacttcacgt
     2161 cccgcctcat cacggctgaa cgtcagcgtc tcatcccccg gcagaatgac ctccgtcagt
     2221 tctccggcgg cgttgtagcg gtacgtggtt ttcagcaggg attcatcggg gtgtctgtcg
     2281 cgttcatctt ccggtggcag gatacagcgg atgacggtgt gttcgtcggg gtagtggtat
     2341 tcaatggccc gtcggccctg agtggtgaca ttcacccgcc cgcgttcgtc atagccggtg
     2401 cgctgtgatg tgccgtccgg gctgaagata cccgtcagcc tgccggattt gtcatacgca
     2461 tactgcgttt tgccttccgg cgtgatgact tctgccaggc ggcctgcggc gtcccggctg
     2521 tagaggatgg tttcctccag gccgttgacc ctggcggtac acaggccatc ggcgtcatag
     2581 cggtaacgcc agaccacgcc gccccagtca tgctcttccg tcacccggcc gcagttatcc
     2641 agaacataaa gatatgactc accctgcggg ttaatgactt ctgtcagttg cagcgtgtct
     2701 ttatcgtagc ggtactgcca gcggtggcct tcaccgtccg ttcgcgccac cggcaaatca
     2761 aacgggccgt actccattgt ccagacaaca ccgttaccgt ccgtgtacgc cagcaggttg
     2821 ccgtgacggt cgtagcggaa tccttccgcg ccggtgccag tgcgctcaag gccgctcagt
     2881 ttatggtgct gctgccactg caaccgcact gactgaccgc cggggccgcg catcttcacc
     2941 ggcaggtcgt gaccactgta ttcggtgtgg tgttcgcggc ccatcacgtc ggtgagtgtt
     3001 tccggccggt tgtggtggtc gtactctatg cccagacgcc gttcatcctg gcggaatatt
     3061 cccgacagca gcccctgagc ggtgtatgcc agccgggtct cttcgccctg tggcgacacc
     3121 agacttaccg gcaggccgcg ttcatcacac tgctgtgtcc acgtctgccc ggcagggtcg
     3181 gtgatgttgc tgagcaggcc gcgctcatca tagcggtact gccactccgc gccgtctgcc
     3241 gccgtgtaac ggcttaactg accgtgtgcg gcgttgtatt caaatttctc cacccgcccg
     3301 gcaggggagt gacgggccag caggtgatgg aattcatccc attcatacat ggtggtgctg
     3361 ccgtccggcg ctgcttcccg cagaatattg ccgtcaggat catagtaata acggtgaatg
     3421 cccttgccgt ccgtcacggt ggtgcagccg tcaccatagt ccagcgtggc gttgtaatag
     3481 ccctccgccc cggtgacatt cacgcaccgg ccctgttcat cgtaggtgaa acggctccac
     3541 gtctggtcgt tatcggacca gcggatgatc cggtccgcag cgtcgtactc ataaaacagg
     3601 tggtagtcca gccgcgcatc cgcttctgtc agccgccccc gcgcatcctg ttcgtaggtc
     3661 gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc gctggcgtgg
     3721 tacgtcagtt taagccggat cccgtcggtg tgactgacac ctgtgatccg gtactggctg
     3781 tcgcgctcaa acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag catgtacatc
     3841 cggtcattgg ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac cgggtggcgc
     3901 agacgcattc ccggaaaggc gtcataaaag aagcggctgc tcagtgcatc cctgtcgaac
     3961 agctccagat aatcatcgcg gcggcgcaga atgtactggg cgtgatacgg gttaaccgcc
     4021 gtatcactgt caccgtcgaa ggcgaagttc agcgtggctc cctggctgat gatatccacc
     4081 cgatttccct gttcacgcac ccaggcgtat tcgctccagg agtcgctcca gcccacgccg
     4141 gtcaggccat gcagcggcag tacagaactg tggaaacggg tgaaggagag ggggagggtc
     4201 tggcccagcg taaagtcggt acgttcgtcg cagtacgcgc cggttaccgg atcgacagga
     4261 tctgttaccc atctgatgac tttattggca atacttttta acgacttacc tgtttttccc
     4321 agcaacgcgc cgagctttgc ccccagcagc ttcccgcttt tccccagcag gttccccagt
     4381 ttgccgagtc tcgccagatt cttcagtttt cccagcagtc ccgctggcgg cagaagatag
     4441 ctggctatcg ttattccccc caggatcatt ctctgccacg gcgggaactc tggctggata
     4501 tccagatagg tcacctgttc accgccaata aagacgtccg gcgaaccttc cacgattgcc
     4561 gcgctgcact ccagtttgtc gcccttgcgc gcggcgggct gattgttgat aaagacgttg
     4621 ctgctgccct gggctatcct cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca
     4681 ccggcttccg ggaaatccac atcttttttg atttcgccac gcccgcccac catggtggca
     4741 aactccatta ataccggacc ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc
     4801 ataaaaccgg cattgctgtc cgtcagggtg ataacagcgt tcccccaggc cttcgccagt
     4861 ggtactgctt tttccgctgc cgattcaagc aggtcagtgg caatatcacc ccatgagggt
     4921 tccccctgtg ggatttcagg tattgctcca ggtgggggag gagggggaag ggtgacggcg
     4981 gccctggcag cgggctttcc gttaatgttt acattttcag aaccggtatt gatggcgccg
     5041 tccgtgtcac aaaagctgtt tatccatgat tcaagctggt tctgtttctc ctccacataa
     5101 tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc ataagggccg
     5161 gtggcacagg cggccataat caggcaacct tctgcgatgg tcactatcgc ccccagcacg
     5221 gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag cctggcggcg
     5281 ggggggccgg acttttttga tgccagcatc tgtgcaatgg ctttgtcgtt ggctgcccgt
     5341 ttcatctggc tggcgaccat cgcctgctgt gacagcattc gcattgccag cgtaccgcct
     5401 gcggtcgctc cctgtggccc gcctggtcct tcactcatgt gttatccctc gtttatgtca
     5461 gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc tctttatcac
     5521 ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga aagtgaaggt
     5581 cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc cctgattccc
     5641 ctcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct ctgccagagt
     5701 gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt
     5761 ttcctggtac agctctggtg tcagggttcc ctgtggaacg gcttcgcggg taatgaccag
     5821 gttgatgcca ctgttatctg tggacaccag gacctgcatt gactgatcct gccattgaga
     5881 cggcagttga atgtggcctt cattaaaagt gaactgcata aaatactccc tgataatctg
     5941 agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct tcgacagcct
     6001 gattaccgtc gattccccga cctgtagctg aatgccatcc ggcgtcagcg caattttgct
     6061 ttcctccacg gccagcaaaa tttccttaat cgccgtataa gcctgtatac cgtcgccttc
     6121 gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa
     6181 gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt tttccaccgt
     6241 aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg
     6301 atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc
     6361 gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat taaatccgct
     6421 gcccatgtac gttttggagc ggatggtcat ctgcgtcttt gttcccggca ggctgccggg
     6481 ggggcggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg gatcgccgtt
     6541 gaggaagtcc acaatgactt cctgacccac acgtggtatc gccaggtggc caaaaccggt
     6601 gcctgcccac gcctgtgcca cacggatcca gcacgaactg tcctggtttg acgggttata
     6661 gcggtcccag ttgaacttca cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc
     6721 tgccggtcct gtcacgacag cgctctgcgg gccgtcgacc agcggtttca gcaacggctg
     6781 tggtcgccat gttctgtctg ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc
     6841 tccctgccgt cctggcaccg cctgtggctg ttcgccgtgc agttcacttg ccaccacctg
     6901 ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgtc gtcccggcca
     6961 tatctccggc gagcgactca ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat
     7021 ctgccagcgg gcaaagttct gcccgtgggc acccttgaaa cgtccggggt agtcatacac
     7081 ttcatactgc gtgcgctggt agtcctggtg ctggccttcc tgttcaaaac gtccggccca
     7141 gccggggcgt ttaaaggtgt agtctttggt caccacggaa gaagggcgga tttgtgcgct
     7201 gtagcggaac tggctgatgc agagggtgct cacctcggta cgggtgttcg ggttccatgg
     7261 gatttcaaaa gattcgggca gatggcggac tgtgtcgcac agcaccaggc tctggtcggt
     7321 acttttgtaa gcatgctcct cataaaagaa gatgccttcc tccgccgcca tccggcacag
     7381 gaaatcgtaa tcagtctcac cgtactggac acaaaactca cgggaaggat gcggctcgct
     7441 gaacagcgga ctccattcgg tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt
     7501 gatgtcctcg ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg
     7561 gtgcaccttc atgctgtaca gcatctggtt tttgtcgttc tccccgagtt caaaccaggt
     7621 caccacgcct ttcacccggc gctgtacttc atcgccctgc catatcgtca ggtaggccat
     7681 tttgtccagc acctgcgcaa attcaaggga gagaaactgc tggctgacca gggagagatc
     7741 gagggaaaag agcgaagaga gtgactggtt cagatgaaag gaaaccaccg caaaagcatc
     7801 cggcggcagg ccgtccactt ccagtgtgaa acgtaatccg gttgacatag caacctccag
     7861 taaaatgaat aatgatccgt accgtacaac tgcggataat gtgataagcg ggacgacttc
     7921 agacgttatt agggaaggaa cagaaaatgc tatggttatg tgagaatttt gtttaaatat
     7981 tactttacag aaacgggtgt ctgttcagtc aaaaaaataa tcacaagctc aacctcttgt
     8041 ttcttggcgt tattcaggac aggtacgcat atcacaacga gtaa
//
LOCUS       DARNVM010000192         8049 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.192, whole genome shotgun sequence.
ACCESSION   DARNVM010000192 DARNVM010000000
VERSION     DARNVM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.192"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..140)
                     /locus_tag="SI621_004168"
     CDS             complement(<1..140)
                     /locus_tag="SI621_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2517 family protein"
                     /protein_id="HEI4066817.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RV"
     gene            453..542
                     /gene="kdpF"
                     /locus_tag="SI621_004169"
     CDS             453..542
                     /gene="kdpF"
                     /locus_tag="SI621_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEI4066818.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            542..2215
                     /gene="kdpA"
                     /locus_tag="SI621_004170"
     CDS             542..2215
                     /gene="kdpA"
                     /locus_tag="SI621_004170"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEI4066819.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTAPGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2238..4286
                     /gene="kdpB"
                     /locus_tag="SI621_004171"
     CDS             2238..4286
                     /gene="kdpB"
                     /locus_tag="SI621_004171"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEI4066820.1"
                     /translation="MSRKQLALFEPTLAVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCICIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVKAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKMLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSHVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4295..4867
                     /gene="kdpC"
                     /locus_tag="SI621_004172"
     CDS             4295..4867
                     /gene="kdpC"
                     /locus_tag="SI621_004172"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEI4066821.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFSGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4860..7544
                     /gene="kdpD"
                     /locus_tag="SI621_004173"
     CDS             4860..7544
                     /gene="kdpD"
                     /locus_tag="SI621_004173"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HEI4066822.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7541..>8049
                     /gene="kdpE"
                     /locus_tag="SI621_004174"
     CDS             7541..>8049
                     /gene="kdpE"
                     /locus_tag="SI621_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEI4066823.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDITVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKV"
BASE COUNT         1662 a         2093 c         2384 g         1910 t
ORIGIN      
        1 cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc
       61 agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag ccatgcagga
      121 tattctctgt agagttccat acttttctcc cccttttgta tctacccggt gaatggcacc
      181 ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact
      241 catccgacca cttatttttg cttattgatg gtttattgac attcacactg taattaagtt
      301 acacaaaagt taaattaata ctaaccatca gttaaatcat ggcttttgcc atttttatac
      361 tttttttaca ccccgcccgc agatttttgc gaaatctttg cagccagaat tctacccttc
      421 cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtgt
      481 tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcattct
      541 gatggctgcg caagggttct tactaattgc cacgttttta ctggtattaa tggtgctggc
      601 gcgtccctta ggtagcgggc tggcgcggct gattaatgat attcctcttc ctggtacgac
      661 gggcgttgaa cgcgtactct ttagcgcgct tggcgtctct gaccgcgaga tgaactggaa
      721 gcagtatctc tcagccattc ttggcctgaa tatactgggg ctggcggtgc tgttttttat
      781 gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccggggc tgtcgtggga
      841 tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat cttacagcgg
      901 tgaaaccacg ttgagctatt tcagccagat ggcgggctta acggtgcaaa actttctttc
      961 tgccgccagc gggattgcgg tgatttttgc cctcatccgt gcgtttaccc gccagagcat
     1021 gagcacactc gggaatgcct gggtcgatct gttacgcatc acgttatggg tgctagtccc
     1081 tgtggcgttg ttgattgcat tgttttttat tcaacaaggt gcgttgcaaa actttctgcc
     1141 ctatcaggca gtgaataccg ttgaaggagc gcaacagctg ttaccaatgg gtccggtggc
     1201 ttctcaggaa gcgatcaaga tgctcggcac taacggtggt ggcttcttta atgccaactc
     1261 gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc tggcgatctt
     1321 cttgatccca acagcgctgt gctttgcctt tggtgaagtg gcgggcgatc gccgccaggg
     1381 gcgcatgtta ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg tggtgatgtg
     1441 ggcagaagtt cagggtaatc ctcatctgct ggcactgggc gcggacagca gcatcaatat
     1501 ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac
     1561 ggcggcttcc tgcggcgcgg tgattgcgat gcatgattcg tttaccgccc ccggtggcat
     1621 ggtgccgatg tggctgatgc aaattggtga agtggtattc ggcggtgtcg gttctggtct
     1681 ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg
     1741 tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga ccgcactggc
     1801 gattctggtc accccgacgc tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc
     1861 cggacgtagc gccatcctca acccgggccc gcatggtttt agcgaagtgc tgtacgccgt
     1921 gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actccccatt
     1981 ctggaactgt ttgctggcgt tctgcatgtt tgttggtcgc ttcggggtga ttattccggt
     2041 gatggcaatt gctggttcgc tggtgagtaa aaagagccaa cccgccagct ccggcacact
     2101 gcctacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc
     2161 actgaccttt atccctgccc tggcgcttgg cccggtggcg gaatatctct cctgatgata
     2221 ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgccg
     2281 ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcacaatgg cgcaatccgg
     2341 tgatgtttat cgtctggatc ggcagtttgc tgaccacctg tatttgcatc gcaatggcaa
     2401 gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca
     2461 ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca
     2521 acagtctgaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg
     2581 gcgctgcggc ggacaaagtt cccgccgatc aactgcgtaa aggcgatatc gtactggtga
     2641 aagctggcga tattatcccc tgcgatggtg aagtcattga agggggcgca tcggtcgatg
     2701 aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc gattttgcct
     2761 ccgtcactgg cggcacgcgt attctttctg actggctggt gattgagtgc agcgttaacc
     2821 ccggcgagac atttctggat cggatgatcg cgatggtgga aggcgcgcag cgacgtaaaa
     2881 cgccaaacga gattgccctg accatcctgc tgattgccct gactatcgtc tttttactgg
     2941 caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgcagtcagt gtaacggtac
     3001 tggtggcgct gctggtctgt ctgatcccaa cgaccattgg cggtctgttg tcagcgatcg
     3061 gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc ggacgtgcag
     3121 ttgaagcggc gggtgacgtt gacgtcctgc tgctggataa aaccggcacc atcacgctag
     3181 gtaaccgtca ggcatcggag tttatccctg cgcagggcgt ggatgaaaaa atgctggctg
     3241 acgccgcaca actggcttca ctggctgatg aaacgccgga aggtcgcagt attgtgatcc
     3301 tcgccaagca gcgttttaac ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg
     3361 taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc atgatccgta
     3421 aaggttctgt cgatgccatt cgccgccatg ttgaggctaa tggtggtcac ttccctgccg
     3481 atgttgatca aaaagtcgat caggttgcgc gtcagggagc cacgccgctg gtagtagtag
     3541 aaggctccca tgtgcttggc gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag
     3601 agcgcttcgc gcagttgcgt aaaatgggca ttaaaacggt gatgattacc ggcgataacc
     3661 gtctgactgc cgccgcgatt gctgcggaag cgggcgtcga tgatttcctc gccgaagcga
     3721 caccagaagc caagctggca ttgattcgtc agtatcaggc agaaggccgt ttagtggcga
     3781 tgaccggcga cggcaccaac gatgctccgg cgctggcgca ggcagatgtc gcggtggcga
     3841 tgaactccgg tactcaggcg gcgaaagaag cgggcaatat ggtggatctc gactctaacc
     3901 cgaccaagtt gatcgaggtg gtacatattg gcaaacagat gctgatgacc cgtggctcgc
     3961 tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcat
     4021 tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact
     4081 ccgcaatcct cagtgcggtg attttcaacg ccttgattat cgtctttttg attcccctgg
     4141 cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat
     4201 ggatttacgg tctgggtggg ctgctggtgc cgtttatcgg tatcaaagtc attgatttac
     4261 tgctgaccgt ttgcggtctg gtgtgaggtt taccatgcgt ggattacgtc cggcattatc
     4321 gacatttctc tttttgttac tgattactgg cggcgtttac ccgctgctga ccaccgcgct
     4381 ggggcaatgg tggtttccct ggcaggccaa cggttcgttg attcgtgaag gtgatacggt
     4441 gcgcggttcg gcattaatcg ggcagaattt ttccggcaac ggctattttc atggtcgccc
     4501 gtcggcaacg gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcggt
     4561 cagtaaccct gagctggata aactaatagc cgcacgcgtt gctgcattac gggccgctaa
     4621 cccggatgcc agcgcgaacg ttccggttga actggtgacg gcatcggcaa gcgggctgga
     4681 caataacatc accccgcaag cggcggcctg gcagatccca cgcgtggcga aagcgcgtaa
     4741 tctcagcgtt gaacagctca cgcaactgat cgcaaaatac agccaacaac cgttggtgaa
     4801 atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcgctgg ataagcttga
     4861 tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact gccgcgccgc
     4921 atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg cgtcgggaag acctgggcga
     4981 tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag
     5041 aaacccacgg gcgaaaagat accgccgcca tgctggaagg gctggctgtt ctgccgttaa
     5101 aacgccaggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc
     5161 gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc
     5221 atcctaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa
     5281 ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc
     5341 aggttcggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg gtgctggtgg
     5401 acttgccccc agacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc
     5461 aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgcgaac
     5521 tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat gcgcgcctgg cgggggcatc
     5581 ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc gggcataaca
     5641 ccggcagcga aaaactggtc cgcgcggcgg cgcggctggc gtcacggctg ggaagcgtct
     5701 ggcacgcggt gtatgttgaa acccctgccc tgcaccgctt accggagaaa aaacgtcggg
     5761 caattctcag cgccttacgt ctggcgcagg agctgggcgc ggagacggca acactttctg
     5821 atccagcgga agagaaagcg gtagtgcgtt atgcccgtga acataatctc ggcaagatta
     5881 ttctcggtcg cccggcctcg cgccgctggt ggcgtcggga aacgtttgct gaccgactgg
     5941 cgcgaatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca
     6001 cgataaacaa cgcgccggat agccgctctt ttaaagacaa gtggcgggta cagattcagg
     6061 gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg cagtggctga
     6121 tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg gtggtggcgc
     6181 tgttttatgg acgctggcct tcggtggttg ccaccgttat taatgtggtg agtttcgatc
     6241 tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct
     6301 tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgcgggt gtgcgttatc
     6361 aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa atgtcgaaag
     6421 ctctggctgt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa tttattgcct
     6481 ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa ttgcagccgt
     6541 taacacatcc gcaaggaatg acgccgtggg acgatgccat cgcgcagtgg agttttgata
     6601 aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg tgtaccgtac cagattctgc
     6661 cgttaaaaag cggcgagaaa acctacggac tggtggtggt agaaccgggg aatctgcgtc
     6721 agttgatgat cccggaacag cagcgcctgc tggagacatt tacgctgtta gtcgccaatg
     6781 cgcttgagcg gctgacgctg accgccagcg aagaacaggc gcggatggca agcgaacgag
     6841 aacagatccg caacgccctg ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg
     6901 tgctgttcgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcgcctcacg
     6961 cccgccaggc cagcgagatt cgccagcatg tgctgaacac gacccgactg gtgaataatc
     7021 tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc
     7081 tggaagaagt ggtcggcagc gcgctgcaaa tgctggaacc gggtttatcg tcgcccatca
     7141 atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccgctc tttgaacggg
     7201 tgctgattaa tctgctggag aacgcggtga aatacgcggg ggcgcaggcc gaaattggta
     7261 tcgatgccca cgttgagggc gagaagctac aactggatgt ctgggataac ggccccggtc
     7321 ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg
     7381 cggtaccggg tgtcgggctt gggctggcaa tttgtcgggc gattgtggat gtacacggcg
     7441 gcaccattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc
     7501 agcaaactgc ccctgaactt gaagaatttc atgaggatat gtgacaaacg ttctgattgt
     7561 tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg acggtatgcg
     7621 cgtctttgag gccgaaacgc tgcaacgcgg cttgctggaa gcggcaaccc gtaagccaga
     7681 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct
     7741 gcgccagtgg agcgcggtgc cggtgattgt gctttccgca cgtagcgaag agagcgataa
     7801 aatcgccgcg ctggatgccg gagcggatga ttacctgagt aagccatttg gtattggcga
     7861 attgcaggcc cgtctgcgcg tcgctttacg tcgccactcc gccaccgctg cgcccgatcc
     7921 gctggtgaaa ttttccgata ttaccgtcga tttagccgcc cgcgtgatcc atcgcggtga
     7981 ggaagaggtg catctcaccc caattgagtt tcgcctgctg gcagtgttgc ttaataatgc
     8041 gggaaaagt
//
LOCUS       DARNVM010000193         8049 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.193, whole genome shotgun sequence.
ACCESSION   DARNVM010000193 DARNVM010000000
VERSION     DARNVM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.193"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..203
                     /locus_tag="SI621_004175"
     CDS             <1..203
                     /locus_tag="SI621_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066824.1"
                     /translation="QRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGTKSQD
                     KHALFPIFTASWRANRIKNKGI"
     gene            426..923
                     /gene="ubiC"
                     /locus_tag="SI621_004176"
     CDS             426..923
                     /gene="ubiC"
                     /locus_tag="SI621_004176"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEI4066825.1"
                     /translation="MSHPALTQLRALRYFTEIPALDTQLRDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            936..1808
                     /gene="ubiA"
                     /locus_tag="SI621_004177"
     CDS             936..1808
                     /gene="ubiA"
                     /locus_tag="SI621_004177"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEI4066826.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1963..4386)
                     /gene="plsB"
                     /locus_tag="SI621_004178"
     CDS             complement(1963..4386)
                     /gene="plsB"
                     /locus_tag="SI621_004178"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEI4066827.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4557..4925
                     /gene="dgkA"
                     /locus_tag="SI621_004179"
     CDS             4557..4925
                     /gene="dgkA"
                     /locus_tag="SI621_004179"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEI4066828.1"
                     /translation="MANNTTGLTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5035..5643
                     /gene="lexA"
                     /locus_tag="SI621_004180"
     CDS             5035..5643
                     /gene="lexA"
                     /locus_tag="SI621_004180"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HEI4066829.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5716..7041
                     /gene="dinF"
                     /locus_tag="SI621_004181"
     CDS             5716..7041
                     /gene="dinF"
                     /locus_tag="SI621_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HEI4066830.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7157..7366
                     /locus_tag="SI621_004182"
     CDS             7157..7366
                     /locus_tag="SI621_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEI4066831.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7408..7923)
                     /gene="zur"
                     /locus_tag="SI621_004183"
     CDS             complement(7408..7923)
                     /gene="zur"
                     /locus_tag="SI621_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HEI4066832.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
BASE COUNT         1802 a         1986 c         2218 g         2043 t
ORIGIN      
        1 cccagcgctt acatgatatg ttgaatccta caaaagatac caattggaat tccacgtata
       61 tctacaaatc cagacatgag atgttgcctg ttaatcttac tccggaaaca cttttcggca
      121 ccaaatctca ggataaacat gctcttttcc caatttttac tgcgagttgg cgagctaatc
      181 gtataaagaa taaaggtatt taagtaaagg aaaacatcat cgttcctggc atcctgggcg
      241 gtgatgtccc tatggttgcc ctcgccagca cgggcatcgg caaagcgtaa ggttcaacag
      301 cgttttacca cttcatgcga ttgttgcgtt tttgttgcgc attagatcac ttaatttgct
      361 ttacatctcc cgtaaacact tttctgcgat acaatgcctt tacgttatgt aacggagagt
      421 tcggcatgtc acaccccgcg ttaacgcaac tgcgtgcgct gcgctatttt acagagatcc
      481 ctgccctgga tacgcaactg cgcgactggc tgttgctgga agattccatg acaaaacgtt
      541 ttgaacagca gggaaaaacg gtgagcgtga cgatgatccg cgaagggttt gtcgagcaga
      601 atgaaatccc cgaagaactg ccgctgctgc cgaaagagtc tcgttactgg ttacgtgaaa
      661 ttttgttatg tgccgatggt gaaccgtggc ttgccggtcg taccgtcgtt cctgtgtcaa
      721 cgttaagcgg gccggagctg gcgttacaaa aattgggtaa aacgccgtta ggacgctatc
      781 tgttcacatc atcgacatta acccgggact ttattgagat aggccgtgat gccgggctgt
      841 gggggcgacg ttcccgcctg cgattaagcg gtaaaccgct gttgctaaca gaactgtttt
      901 taccggcgtc accgttgtac taagaggaaa aaaatatgga gtggagtctg acgcagaata
      961 agctgctggc gtttcatcgc ttaatgcgta cggataagcc aattggcgcg ttactgctgc
     1021 tctggccaac attatgggcg ttgtgggtgg cgacaccggg cgttccccag ctctggatcc
     1081 tggcggtgtt tgtcgcgggt gtctggctga tgcgcgctgc cggatgtgtg gtgaatgatt
     1141 atgctgaccg caagtttgat ggtcatgtta agcgcacggc gaaccgaccg cttcccagcg
     1201 gcgcggtaac ggagaaagag gcgcgcgcgc tgtttgtcgt gctggtactg atttcgtttt
     1261 tactggtgct gacgctgaat acgatgacca ttctgttgtc gattgccgcg ctggcgctgg
     1321 cgtgggtgta cccgtttatg aagcggtata cccatctacc gcaagtggtg ctgggcgcgg
     1381 cgtttggctg gtcgattcca atggcttttg ctgctgtgag tgagtcggtg ccattgagtt
     1441 gctggttaat gttcctcgcc aatattctct gggcggtggc ttacgacacg cagtatgcga
     1501 tggttgaccg cgatgatgat gtgaagattg gcattaaatc caccgcaatc ctgttcggcc
     1561 aatacgataa attgattatt ggtattttgc agattggcgt actggcgctg atggcgatca
     1621 tcggtgagtt aaatggctta ggctggggat attactggtc aattctggtg gctggcgcgc
     1681 tgtttgttta tcaacaaaaa ctgattgcca accgcgagcg tgaagcctgc tttaaagcat
     1741 ttatgaataa taactatgtt ggtctggtac tgtttttagg gctggcaatg agttactggc
     1801 atttctgatg atgtaaaaaa gccggatgat catccggctt ccttctgggt tgcctgatgc
     1861 gctgcgcttc tcaggcctac acaacatatc gcaatttatt gaatttgcag atcatggtag
     1921 gccgaataag gcgtttgcgc cgcatccggc aattctccct gattaccctt cgccctgcgt
     1981 cgcactctca atcgtcaaac gcacgtctga tgtaatcaac tccgccagca actgatacac
     2041 cttcatcgtt tctgccggtt cggcgtcgcc gctatcgctg atataccctt catcacgcag
     2101 tgttagcacc agagaactga acaccgcctt gtcgaagaac tccggcgcgt tgataccgtg
     2161 cagcacggag agacgttgcg cgacggtgcg gctctctttc tccagcgtac cgcggttgat
     2221 cgacgggttg gcgctcaaca gccagaaggt aatggcataa cgttgcagcg tttcgcgcgc
     2281 gcctgcggcc agcagctgta gcgtgcgaga atgcgccggg ttgatatgca actcatcatc
     2341 ttgcagggta atcagcccct gacgttgcat ctcatttgcc agtgcatcaa taacgtccgg
     2401 caactcgtcg cgatcccagc gcaggaacag ctccgctttc agcattgggt aaagcacatt
     2461 gacgtgctcc atcaatacgt cgcgggagat gtggcgatgc tgggtgacaa ttgccgccat
     2521 cagcgaaggc agcaccaaca tatgcgcaat gttgttgcga tagtaggtca tcagcaccgc
     2581 ttgctcgcgc ggcagaatga tgatgtcgcc gatagtgtct ttctcaactt caaacttgtt
     2641 catttgcagc gcgtgatcga taagctcgct ggcgctggct gaaggaacgg tagagtccgt
     2701 ggagtaaggc acgttgcgca tcagatccag gtagcagttg agttgctcgg ttaactgctc
     2761 gcgggtgagt gagcgctgac gtgacgccag aagcgcagta cagcacaggt tcatggcgtt
     2821 tgccgcgcct gcgttgttaa tgcgtaccat caggtcggca gcaatattat tgaccgtcgg
     2881 cgttaaccag gccggacgca ccgcttcgat gggatcgata gattcacgcc agtctggtac
     2941 gtgctggtta aggtaggtca tcaacggcat tggttcaccg aagttgacgt aaccctgacc
     3001 gagattacgc agcttgctta aaccgcgcag catctgcggc aggctctctt tctctttcgt
     3061 tgcaccgcgc agttctttgg cgtaagtacc cacttccatg acgtgctcat aaccgatgta
     3121 gatcggaatc agcgtaatcg gacgcgtgcc gccacgcagc atcgcctgaa tggtcatcga
     3181 tagcgttccg gttttcggat ccagcaaacg ccccgtacgg gaacgaccgc cttccacgaa
     3241 atactcgacg gaataaccac ggctgaacag ttcgccgaga tactcacgga aaacggtgga
     3301 atagagctta ttgcctttaa acgtacggcg aataaagaac gcccccagac ggcggaaaat
     3361 cggcccggca ggccagaaat tcaggttgat cccagcggcg atatgcggcg gcaccagccc
     3421 ctggtgatac agcacgtaag aaagcagcag gtagtccatg tgactgcggt ggcaaggcac
     3481 atacaccagc tcatgaccgt cgtgggccaa ctggcgaacg cgctcggcgt tatggacgtt
     3541 gataccctgg taaagtcggt tccaggtgaa gcccagaata cggtcagtca ggcgaatcat
     3601 ctcgtaagag aaattcgccg caatctcttc catcagtgca atcgcgttct gctgcgcttt
     3661 ttcatgggag atttttttgc tgcgcgcttc atcttctacc gctttcgcaa tggcgcggga
     3721 ggcgagcagc ttattaaaca gatcctgacg agcaggcaaa cgtgggccta cggcagccag
     3781 acgttgacgg gcaaagtgca tacgcgccac gcgcgccagt ttctgcgcga tagttttatc
     3841 cgtgccgtgt tcatccgcca tacggcgcag cgaaactgac ggtgagaaac gcacaaaact
     3901 gtcgcgaccg agccacagta cagcgaaaaa tttctgtacg ccgttaagca tacgcagcgg
     3961 cgggttcact tcgccttttt cacgccccgg cgcgcgacca aacatcaccg acactggcac
     4021 catctgcaca tccagatttg ggttgctacg gtgcaaatcg agatagtcgt ggaacagctt
     4081 aatagactct tctttcggcg tgtaataggt gaatacacgc ggcccgccgt gaatgaacac
     4141 atagcgcggc agtagcgtgc cgtcgatttc cagcggctct aacgggtcag gcaggtcatg
     4201 tgccagacac tgggcgcgca acgtcagcaa gtctgctttc gagttgtacg gtaaaacgta
     4261 cataattgga cgagaggtat ccagccccag ttccggggca ggatctgccg gaatagactt
     4321 gctttttacc aggatgctta atggtaaatt cagtaatttg tagtaaattc gtggccagcc
     4381 ggacataaac gatgtaaagc ctctggttaa taatgcaaat gcgcggcaag gatagcagaa
     4441 agtcatggga aattctgtgg tatccgctca tgtttcgcgc ggcgttacgc aaacccgaat
     4501 catcggattt aacggtacac tgatattgac gctcataatg taaaaaggtt ctttcaatgg
     4561 ccaataatac cactggactc acccgaatta tcaaagctgc tggctattcc tggaaaggtt
     4621 tacgcgctgc atggatcaac gaagcggcat tccgtcagga aggcgtagcg gtattgttgg
     4681 cggtggtcat cgcctgctgg ctggatgtgg acgcaattac ccgcgtgctg cttatcagct
     4741 ccgtgatgct ggtgatgatt gtggaaatcc tcaatagcgc catcgaagca gtggttgacc
     4801 gaattggctc tgaataccat gagctatccg gacgcgctaa agatatggga tccgccgcgg
     4861 tgctgattgc gattatcgtc gccgtgatta cctggtgcat cctgttatgg tcgcattttg
     4921 gataaccctt ccagatttcg ataaatctct ggtttattgt gcagtttatg gttccaaaat
     4981 cgccttttgc tgtatatact cacagcataa ctgtatatac acccaggggg cggaatgaaa
     5041 gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca
     5101 ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg
     5161 gctgaagaac atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca
     5221 tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg
     5281 gctgccggtg aaccgcttct ggcgcaacag catattgaag gtcattatca ggtcgatcct
     5341 tccttattca agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagat
     5401 atcggcatta tggatggcga cttgctggca gtgcataaaa ctcaggatgt acgtaacggt
     5461 caggtcgttg tcgcacgtat tgatgacgaa gttaccgtta agcgcctgaa aaaacagggc
     5521 aataaagtcg aactgttgcc agaaaatagc gagtttaaac caattgtcgt tgaccttcgt
     5581 cagcagagct tcaccattga agggctggcg gttggggtta ttcgcaacgg cgactggctg
     5641 taacatatct ctgagaccgc gatgccgcca ggcgtcgcgg tttgtttttc atctctcttc
     5701 atcaggcttg tctgcatggc attcctcact tcatctgata aagcactctg gcatctcgcc
     5761 ttacccatga ttttctccaa tatcaccgtt ccgttgctgg gactggtcga tacggcggta
     5821 attggtcatc ttgatagccc ggtttatttg ggcggcgtgg cggttggtgc aacggcgacc
     5881 agctttctct ttatgctgtt gctgtttttg cgcatgagca ctaccgggct gactgcgcag
     5941 gcttatggtg ccaaaaatcc tcaggcatta gcccgtgcgc tggtgcaacc gttgctgttg
     6001 gcgttggggg ccggggcgtt aattgcgctg ctgcgtacgc cgattatcga tctggcgctg
     6061 catattgttg gcggcagcga agcggtgcta gaacaggcgc ggcgctttct tgaaatccgc
     6121 tggttaagcg caccggcgtc gctggcgaat ctggtattac ttggttggtt actcggcgtg
     6181 caatatgccc gtgcaccagt aattttgtta gtggtcggca atatcctcaa cattgtgctg
     6241 gatgtctggc tggtgatggg gctgcatatg aacgtgcagg gcgcggcgct ggcgacggtt
     6301 attgcggaat atgcaacatt gctgattggt ctgctaatgg tgcgtaaaat cctcaaactt
     6361 cgcggaatat ccggcgaaat gctgaaaact gcctggcgag gaaacttccg tcgcttgctg
     6421 gcgcttaacc gcgatatcat gctgcgctcg ctgttgttgc aactctgttt cggcgcgatc
     6481 accgtacttg gcgcgcgact ggggagtgac attatcgctg ttaacgcggt tctgatgacg
     6541 ttactcacct ttaccgccta tgcgctggat ggttttgcct acgcggttga agcacattct
     6601 ggtcaggcgt acggtgcgcg cgacggtagc cagttactgg atgtctggcg ggcagcgtgc
     6661 cgccagtcgg ggattgtagc gttactgttt tcggtggttt atttgctggc aggggaacac
     6721 atcattgcgt tactgacgtc gttaacccag attcagcagc tggccgaccg ctatcttatc
     6781 tggcaggtga ttttgccgtt ggttggcgtc tggtgttatc tgctggacgg catgtttata
     6841 ggcgcaacgc gtgccgccga aatgcgtaac agtatggcgg tggccgccgc aggttttgcg
     6901 ctgacgctcc ttacgctgcc gtggctgggt aatcatggtt tgtggctggc attaaccgtc
     6961 tttctggctt tgcgcgggct ttctctggcg gctatctggc ggcgtcactg gcgcaatggt
     7021 acctggtttg ccgcaacgtg acggttaaaa attctgaata aataatccta agccaaattg
     7081 ctgactacac ttaatctcac gttcagaaga aaagtgaacg tactctcatt cacaacctaa
     7141 cgatgaggtc ttgattatga ataaagatga agccggcggt aactggaaac agtttaaagg
     7201 taaagtgaaa gagcaatggg gcaaactgac cgatgatgat atgacgatca ttgaaggtaa
     7261 acgtgatcaa ctggtcggta aaatccagga acgttatggt tatcagaaag atcaggcaga
     7321 aaaagaggtc gtggattggg aaacccgcaa tgagtatcgc tggtaattaa tccctcctgc
     7381 ccgacgtgta caaggatgta cgccctctta acgtgatttc tttttcacct gtacagagtg
     7441 atcatgctgg cactgttcag gatgacgaca cgcttccact tccacgcatg ccgcacagag
     7501 tccatgtgct tcaatcacat tatgccgcag ggcaaacccc attttggccg ccagcgtatg
     7561 cataatgtct tccacgcctt ctgcacattc ttctttcacc gcgccgcagc gatcgcaaat
     7621 aaacatggct gaagtatggg tgggctggtc gaacagatga cagagcacat agctgttggt
     7681 ggattccacc ttatgcacaa aaccttgctc aagaagaaaa tccagcgcgc gataaaccgt
     7741 tggcggcttg gcttgcggct cagcttcgcg cagcaaatca agcagatcat aagcgctgat
     7801 agcgccatcc tgcagactca tcaggcgcaa cacttccagg cgctgtgggg tcaggcgcac
     7861 attacgctgc gcgcagattt tttcagcctg cgctaataac tcctgcgttg tggtcttttc
     7921 catcgggcac ctcaaagtgg gggttaagaa tgtccttact ttaccatgtt ccaggaaaaa
     7981 caccgagaac ccttattgtt gccgtaatgt tgattttctg ttttgtacgt aaggtgttgt
     8041 tttgcctgg
//
LOCUS       DARNVM010000194         7942 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.194, whole genome shotgun sequence.
ACCESSION   DARNVM010000194 DARNVM010000000
VERSION     DARNVM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.194"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            3..686
                     /locus_tag="SI621_004184"
                     /pseudo
     CDS             3..686
                     /locus_tag="SI621_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
     gene            905..2233
                     /locus_tag="SI621_004185"
     CDS             905..2233
                     /locus_tag="SI621_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEI4066833.1"
                     /translation="MKIANIRLTNFRCFDEFSVDFDPRLTVLIARNGRGKSTLLDAVA
                     LALGPFLTRLPGIKGLSFKKTDFRIDENGDQPSYMRVSCTSTTGITWDRTERRDLTKK
                     TLGSIPQGAALKALNDYVDTFIDSHNENKHYTLPIFVYYGTGRGVFDIPLRKRAFNKH
                     FSRFEALDGALESKTNFKRFVQYFYFLEEKENRLQKEQRSFEVEIPELRAIRLAVEKL
                     LPEFSNIRSAVPAGIMVDWCKPIGNGETTIQKLRIEQLSDGYRTTLAMVMDIAARMAE
                     ANPYSSDPLSSEGIILIDEVDLHLHPEWQREFLPRLINVFPHLQFIVSTHSPFIIQSV
                     KEGMLVDLDKDDISDSPSLNKELSIEDIAEGVMGMEKVQRSELFNKQVAVSDRYYQLL
                     NEGRSESDPEVRKLSAELDKLEEFFGDNPAYVALLRAERRKIMGTQGSKQ"
     gene            2230..2841
                     /locus_tag="SI621_004186"
     CDS             2230..2841
                     /locus_tag="SI621_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEI4066834.1"
                     /translation="MRPVNRPPYNGTVYADYKSFLPSLLTAYGGYCSYCERPDKVDVE
                     HVVPKTHAPLLITDWNNFLLGCPRCNRDFKKSKNTQRIGYVWPDVENTFNLLTYHPDG
                     RVEPKQGLSAGLTAQVQATIDLVCLDDGNQVQKTLNLGRRRYFGLANKAKTLYLSNLL
                     SIDEVMDMAQLGYWSIWYNVFQGVPAVLSALENSYPNTAINRP"
     gene            3267..3932
                     /gene="yecA"
                     /locus_tag="SI621_004187"
     CDS             3267..3932
                     /gene="yecA"
                     /locus_tag="SI621_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HEI4066835.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERLEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3994..5205)
                     /gene="tyrP"
                     /locus_tag="SI621_004188"
     CDS             complement(3994..5205)
                     /gene="tyrP"
                     /locus_tag="SI621_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HEI4066836.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVISVQF
                     LIAAGLLPEVG"
     gene            5395..5634
                     /locus_tag="SI621_004189"
     CDS             5395..5634
                     /locus_tag="SI621_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HEI4066837.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     DGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            complement(5672..6169)
                     /gene="ftnA"
                     /locus_tag="SI621_004190"
     CDS             complement(5672..6169)
                     /gene="ftnA"
                     /locus_tag="SI621_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HEI4066838.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6340..6663)
                     /locus_tag="SI621_004191"
     CDS             complement(6340..6663)
                     /locus_tag="SI621_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HEI4066839.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVGEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNMEFTLSYQCHHSA
                     FPVFL"
     gene            6926..7012
                     /gene="azuC"
                     /locus_tag="SI621_004192"
     CDS             6926..7012
                     /gene="azuC"
                     /locus_tag="SI621_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HEI4066840.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7127..7378
                     /locus_tag="SI621_004193"
     CDS             7127..7378
                     /locus_tag="SI621_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HEI4066841.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7458..>7942)
                     /locus_tag="SI621_004194"
     CDS             complement(7458..>7942)
                     /locus_tag="SI621_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HEI4066842.1"
                     /translation="LKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLRAQAQSNV
                     TQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLAQLADEAK
                     ELNDDSTANFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLNVVSHQLH
                     "
BASE COUNT         2289 a         1733 c         1885 g         2035 t
ORIGIN      
        1 atatgcaaaa atccttccga ttcaccaatt catccatcaa agcccttccg gcaaacacag
       61 atacccgatc tacagagctt gaggtatctg ataccgaagt cattggcctc aaatgcctct
      121 caggtcgaac gggcaacaaa cgtttcctcc tgcgctacac attccacggt accaaaaaaa
      181 gtatcagtat tggcagattc cctgaaatcg atgtgggaac agcccgtcag atagctcgtc
      241 gtcacaagga ggcgattgca ctgggaaatg accctaaggc agagcgcgat aattatcggg
      301 cgatgccaac gcttagtgag ttttttcacc agacctatgt tccgttcatc aagcgccata
      361 aaaagtcctg ggataaggat gttcagcgtt tcacgcaata cattgaacct cgtctgggga
      421 agatacgtta ctgcgacctt caggcccggg aaatccagca ggtgcttttt gacatgcttg
      481 agggccgtat tcacggccag cagtatgcac cgtctacctg taacagggca ctggctattc
      541 tgaaaaccat gggccgttat gcgctacgac aggatgtcct ggagaagaat gaagccgata
      601 agatcccttt gctgaaggag aataatcacc ggacgcgctt ttttgacgct gatgagatca
      661 ggagaatcct tgatgctgcg tctcgataac ccaataaagc tgccggaggg ctgacgccgg
      721 aagagtcaga gaacagatac catttttact gtaaaaccgt ggccagtatt acttgaccac
      781 tacattctaa ggcttgcttt aatgctaagg ctaaatgatt ttgatatagt aagttcatac
      841 ttagcgggca gatgcccaca gacatagtac cgaaaataaa tcaggtattc tgcgagattt
      901 cagtatgaaa atagccaata ttagacttac gaattttaga tgttttgacg agtttagtgt
      961 tgattttgac ccaagactta cagttctcat tgctcgtaat ggtagaggga aatctactct
     1021 gcttgacgcg gtagcattag ctttaggacc atttttaact cgtttgccag ggatcaaagg
     1081 gctaagtttc aagaaaaccg atttccggat cgacgaaaat ggagaccagc cgtcctatat
     1141 gcgagtaagt tgtactagta caacgggtat aacttgggat agaactgagc gaagagatct
     1201 gacaaaaaaa acgttaggta gcatccccca aggggcagcc cttaaagcct tgaatgatta
     1261 cgtcgatacg ttcattgatt ctcacaatga gaataaacac tatacattgc caatttttgt
     1321 ttattacgga actgggcgtg gagtgtttga tataccactc agaaaacgtg catttaacaa
     1381 gcatttttct cgatttgagg cccttgacgg tgctcttgaa agcaaaacaa atttcaaaag
     1441 atttgtgcaa tatttttatt ttcttgaaga aaaagaaaat agattacaga aagagcaacg
     1501 atcatttgaa gtagaaatcc ctgaattaag ggcaatacgc ttggctgtgg aaaaattact
     1561 gccagagttt agcaatattc gctcggcagt acctgctgga attatggtcg attggtgtaa
     1621 gcctattgga aatggtgaaa caaccataca aaagctaagg attgaacaac ttagtgatgg
     1681 ctatagaaca actttagcta tggttatgga tattgcagca agaatggcag aagctaaccc
     1741 ctattcgagt gatcctctaa gttctgaggg gataattctc attgatgagg tagatctgca
     1801 tctgcatccg gaatggcaac gtgagtttct ccctagatta atcaacgttt tccctcatct
     1861 tcagttcata gtatcgacgc attcaccatt tatcatccag tctgtcaaag aggggatgtt
     1921 ggtcgatctt gataaagatg acatttcgga tagcccctct ttgaataaag aactcagcat
     1981 cgaagatatt gctgagggtg taatgggaat ggaaaaagtt caacgaagtg aattgtttaa
     2041 caaacaagtc gcagtttcag atagatatta tcagttactg aatgaaggaa gaagtgaaag
     2101 cgacccagag gtaaggaagc tctccgctga gcttgataaa ttggaagagt tttttggaga
     2161 taacccagct tatgtagcct tacttcgtgc tgagcgccga aaaataatgg ggacacaagg
     2221 aagtaaacaa tgagacctgt aaatcgacct ccatataatg gaactgttta tgcagattat
     2281 aaatcatttt taccttcgct attaactgca tatggcggat attgttctta ttgtgagcgg
     2341 ccggataagg ttgatgtgga gcatgttgtc ccaaaaacgc atgcaccact tttgattact
     2401 gattggaaca attttttact gggttgccct cgctgtaacc gagattttaa aaaaagtaag
     2461 aatactcaac gtataggcta tgtttggcca gatgttgaga atacatttaa tttattgaca
     2521 taccatccag atggtagagt agagcctaag caggggctgt ctgccggact aactgcgcag
     2581 gtacaggcta ctattgattt ggtatgtctt gatgatggta accaagtaca aaaaacttta
     2641 aatcttggta gaaggagata ttttggactc gcgaataagg ccaagactct ttatctgagt
     2701 aatttattga gtattgatga ggtcatggat atggctcagt taggatattg gtcgatctgg
     2761 tataatgtgt ttcaaggtgt acctgctgtc ctttcagctt tggagaattc ctatcctaat
     2821 accgctataa accgcccatg acataggtga aatgaattgg ctcaggtgcg tacaagggga
     2881 agacttacct gtgcagcttc tgtgttcaac gttatccatg tctatctatt tactgccaat
     2941 tacagtaaag ttggtggata attacagcaa agtgttgcaa tgggttgcta tgtaaatcat
     3001 tgatcctgcg tagaatttaa aatccctcgg cgttcgcgct gtgcgggttc aagtacccgc
     3061 tccgggtacc atgggaaaga taagaataaa atcaaagcaa taagcagtgt cgtgaaacca
     3121 ccttcgggtg gtttttttgt gcctgcaact tgtcgttaca ccctccttaa tttttaatca
     3181 ccggcaaagc cactcaaaaa gtatctctgt tacggaccga catgtaagat aggtgctggc
     3241 gagttgagat ccacaaggaa aagcgtatga aaacgggacc gttaaacgaa agtgagttgg
     3301 aatggctgga cgatattctg accaaataca acactgacca cgccattctt gatgtggcgg
     3361 agctggacgg tttattgacg gcggtgttga gttctccgca agagattgaa ccggcacagt
     3421 ggctggttgc cgtgtggggt ggggctgact atgtgccacg ctgggcgtca gagaaagaga
     3481 tgacgcgctt tatgaacctg gcttttcagc atatggctga taccgcagag cgtctgaacg
     3541 aattcccgga gcagtttgag ccgttattcg gcttgcgaga agtcgatggc agcgagctga
     3601 cgattgttga ggagtggtgc tttggctata tgcggggcgt ggcactttct gactggtcaa
     3661 cgttgcccga ttcgttaaaa ccagcgctgg aggcgattgc gctgcacggt actgaggaaa
     3721 acttcgagcg gttagagaag atgtcgccag aagcgtttga agagagcgta gatgccattc
     3781 gactggcggc gcttgatcta catgcgtact ggatggcgca tccgcaggaa aaagctgttc
     3841 agcaaccgat caaagcagaa gagaaaccgg ggcgtaatga tccttgcccg tgcggtagtg
     3901 gtaagaaatt taagcagtgc tgcctgcatt aagagatcaa attgggtgtc agcgttaatt
     3961 ctggcaccca ataaggaatt tgaggctatc tgatcacccc acttctggta acaagcctgc
     4021 tgcaatcaaa aattgcacgc taatcacagc aataccacag agaaacacca ccaccagcgc
     4081 cggacgacca cctttcaccc ggtaacccgc ctgaggattg tgctttctgc tttgccaggt
     4141 caacagcgaa gggataatca atgccagtac cgccagcgcc acaccggcgt aacccagcgc
     4201 catcacaaat cctcgtggat aaaacagtgc aaacgccaac ggcggcagaa aggtaattgc
     4261 accagtttgc aatcgtccac caacggtatt tgaacgctga aacaaatctg ccagataatc
     4321 aaataaacct aacgcaacgc cgagaaatga cgtggcgagg gctaaatcag caaataaatg
     4381 caccgccagc tcaacatgcg gagaggccac catttcgcgt aatgcctgta acagcccgtt
     4441 taatccggca tgattagcca gcaatcccat aaaggttgtt gaatcaatgc tgccaagcgt
     4501 cgccacctgc cagaaaatat atgccaccag ggggatcgca ctaccgatta taaacaccca
     4561 gcgtagctta cgaatgttgc catccatata gctgacaata ctcggcacgc taccgtgaaa
     4621 accaaacgac gtaaaaatca ccgggattgc agatagtgcg agcccctgtt gcaacggcag
     4681 ggttaaaaga ttcaccttat gaatatgcgg aagtagcagt accagcatca ccaccagaaa
     4741 aataattttg gcgctgaaca gaaaacggtt aaataaatcg accagcgaag ttccgacaca
     4801 aaccacgcca ccggcaacaa aagtgaacaa cagcacgcca gcggttgccg acattgaaat
     4861 acctgtccag tcgctgatgc tggaagccaa caattcacca gcaccgctga tgtatgccgc
     4921 agtcagagca tacattaaga acatcatact gaagcccgtc agccactgac cgtagcgtcc
     4981 cagatagcgt tttgccagcg tgccaagacc ggtatctgcc ggaacatgct ggtacacctc
     5041 cagcagtaat agcgccgtgt agcacatcaa cgcccaaagc ccaattaaca agattaacgt
     5101 gacgctaaaa ccaacacctg ccgcagccag cggcattgcc agcatgcctg cgccaattgt
     5161 ggttcccgcc acgataaaaa cacttcccag agttctgttt ttcacgcttt cttctgtcct
     5221 gacaatcttt atgagtgata tctgcggcgc aggttaaggc ataacggctg cttcgtcaaa
     5281 ccgacgttac atatagtgta aatataaatg tacaataaaa gcgtttagtt ctgcgctggc
     5341 gcaaagtctt cataagcggc aatcgctacg ctagcctttt tttgaggagg aacgatggac
     5401 tctattcacg gtcatgaagt gttaaatatg atgattgaat caggtgagca atatacgcat
     5461 gccagtctgg aagctgcgat taaagcgcgt tttggtgaac aggcacgttt tcacacttgc
     5521 tcggcagacg ggatgacagc gggagagctg gtagcgtttc tggcagcaaa aggcaaattt
     5581 ataccttcgg aagacggttt ttcgaccgat cagagtaaga tttgccgtca ctgatttgaa
     5641 acggcggcag aaaacctgcc gcctgagagc attagttttg tgtgtcgagg gtagagagtt
     5701 ctttgtcgat aaaatacaga ccttcgccgc ttttgcctgc caggcttaat ttatcaataa
     5761 tcgatttgaa cagtttctct tcttcatgct gctcagaaac ataccattgc aggaaattaa
     5821 atgttgggta gtcctgattg gtcattgcag catgagccag ttcgttaatt ttctgcgtga
     5881 ttaattgttc gtgtttatag gtttcctgga ataattcatc aagtgaggaa tattcagcaa
     5941 acggagattc aacggtatta atacgcggta agttgccggt atcagtcaga taatcaaaca
     6001 gacgctgcat atgcgtcatc tcttcctggg cgtggcggcg caggaacgcg gcagcacctt
     6061 cgaaggtatg atagctgcac caggcgctca tttgctgata aagcagtgaa gagtacagtt
     6121 ccaggttcat ctgctcatta agtttttcaa tcatttctgg tttcagcatg atagtgctcc
     6181 acaaaggtta tatttgttgc tgtggcgtca ctataatttg ttattttatt atttgcaaaa
     6241 ggtaaaatat aaacttcatt tattaaaaat acgaatggga ataaaacgca ttaccgctat
     6301 ttatttaatc agcgaagatc ccctcgctga ttaaaacatt tagagaaaga cagggaaggc
     6361 ggaatgatgg cactgatatg acaaagtgaa ttccatattc aaacataagg aacccgcaaa
     6421 aaggctacag tttcctactg gctgaccaaa ggggatcgcg tgggtatagc cttcctgctg
     6481 gcatgcgcga tcggcgatcc cgcgactgac ataacgatcg gtatgggcat gctgcaaaaa
     6541 agcctgatca taaaccaacc gaacaatacc tgttgccgcg tcaacttcgc ccacatgagc
     6601 ctggcgagta actgtgcacc ccgccagcgt tattaatgaa agagtcagaa tcagaagtct
     6661 catggtccag ccagaagagt aaaagaaagc actattttac gcgttacagc gctgcgacaa
     6721 tggccggaaa agacgcctgg caggcgctat ttctcgccat ctgtatgtgt cagaaatggg
     6781 ggggctgctt tttgattatc atttttataa cattaaaggg gtatttatgt gatttgcatc
     6841 acataaagat tgatttttgt gatggttgtc atattataag tgcaaagaca ataacacctg
     6901 cataacaaat aatcggagtg ccgcgatgaa actgcgcaag atcctgaaaa gtatgttcaa
     6961 taactattgc aaaacgttca aagacgtacc accaggcaat atgttccgat agcaaaaaac
     7021 ctgctccggc aggttttttt gtgtcctgat gacggtggat gtgactatcg ctgaagatgt
     7081 gaagccgcta ctataacggc tttcaaaatg ctatcaggag tttacgatgt cccagccgct
     7141 gaatgccgat caggaactgg tttctgatgt ggttgcttgc cagttggtta tcaaacaaat
     7201 tctggatgtt ctcgatgtaa tcgcgcccgt tgaagttcgt gaaaaaatgt ccagccagct
     7261 gaagaatatc gactttacgc accatcccgc cgccgcagac ccggtcacca tgcgggcaat
     7321 ccaaaaagcc atcgcgctga ttgaactcaa gtttacccca cagggcgaat cccattaaaa
     7381 aaaccgctgc ttcaaaaaga tgaggcagcg gtttctcaaa aacttcatcg gcgcaatgca
     7441 ttagcgccga tgtatgatca atgcagctga tgcgacacga cattcagaac atgttgatcg
     7501 gtctgcacag ggcacatacc cgcaagtttc gcactgcgca cttcatcaag aatggtttgc
     7561 agcaacaggc catcatgctg ctgttctttt tccagatcgc gcaggaaatt ggcggttgaa
     7621 tcatcattca gttctttcgc ttcatcggct aactgtgcca acgtactaga acgttgctcg
     7681 tattcttcca tcgttttttg gaaaagttct tccagtgagt tcagtttttc accgggaaca
     7741 tcaatggctt taacgatggg ggtagcgccg acactcttca taaagttaaa catgcgcatc
     7801 atttgggtca cattactctg tgcctgggcg cgaaggaaag tggcggtgcc gttcagactc
     7861 tgttcagaac accagttact caggtgaagg tagagattgg atgcgtaaaa ctcgcggttc
     7921 atttgagagt tgagtttgag aa
//
LOCUS       DARNVM010000195         7811 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.195, whole genome shotgun sequence.
ACCESSION   DARNVM010000195 DARNVM010000000
VERSION     DARNVM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.195"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..476
                     /locus_tag="SI621_004195"
     CDS             <1..476
                     /locus_tag="SI621_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066843.1"
                     /translation="GMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEQNYIHPVGSI
                     FAAIQKNESLLKFPSVISMLNVNLLFNPLNLPGMGSGVLEEIMSISDSSLRKRLGYEV
                     LSFSLQAHSLSQECIDKLAIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(480..1871)
                     /gene="tcyP"
                     /locus_tag="SI621_004196"
     CDS             complement(480..1871)
                     /gene="tcyP"
                     /locus_tag="SI621_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HEI4066844.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2004..2594)
                     /locus_tag="SI621_004197"
     CDS             complement(2004..2594)
                     /locus_tag="SI621_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEI4066845.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2757..3425)
                     /gene="hxpB"
                     /locus_tag="SI621_004198"
     CDS             complement(2757..3425)
                     /gene="hxpB"
                     /locus_tag="SI621_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEI4066846.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3572..4108
                     /locus_tag="SI621_004199"
     CDS             3572..4108
                     /locus_tag="SI621_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HEI4066847.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4149..5009)
                     /locus_tag="SI621_004200"
     CDS             complement(4149..5009)
                     /locus_tag="SI621_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEI4066848.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5115..5405)
                     /gene="ghoS"
                     /locus_tag="SI621_004201"
     CDS             complement(5115..5405)
                     /gene="ghoS"
                     /locus_tag="SI621_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HEI4066849.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(5506..6435)
                     /gene="pfkB"
                     /locus_tag="SI621_004202"
     CDS             complement(5506..6435)
                     /gene="pfkB"
                     /locus_tag="SI621_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HEI4066850.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6722..7480
                     /locus_tag="SI621_004203"
     CDS             6722..7480
                     /locus_tag="SI621_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HEI4066851.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7533..7640)
                     /locus_tag="SI621_004204"
     CDS             complement(7533..7640)
                     /locus_tag="SI621_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066852.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         2046 a         1896 c         1885 g         1984 t
ORIGIN      
        1 ttgggatgga ttttacctgt gaattccgac cagaaaaaat gaccaatttg atcaatataa
       61 aaaacaggtt ggttattcac tatttaagaa cgatatacta tgaacaaaat tatatacatc
      121 ctgttgggag catttttgct gcgattcaaa aaaatgagag tctcctaaag tttccttcgg
      181 taatatcaat gcttaatgtc aatcttcttt ttaatccact gaatcttccg ggaatgggaa
      241 gtggtgtgct tgaagagatt atgagtattt cagatagttc acttagaaaa cgtcttgggt
      301 atgaggtttt aagcttctcg ctacaagcac attcattgag tcaggaatgt atcgataagt
      361 tagccatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg
      421 aacatttaaa aagtaaggca acggccccta tacaaaacgg accgttgcca gcataagaat
      481 taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc
      541 agccactggc tggtcagcgt gccagcggtc atcgagccac taacgtttag cgccgtacgc
      601 cccatgtcga taagcggttc aacggagatt aacaatgcca ccagcgttac aggcaggccc
      661 atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct
      721 gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc catcgggtta
      781 atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcacagccg
      841 ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacaccc
      901 agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta
      961 aaggcaaacg tcagaactgg ccatacctta cggaagtact tcagcgggct cacgccatta
     1021 atacccagca gaaggccatg cactgcaaac ataatcagaa gaccgaggta ggacgcgaca
     1081 acgaaacttc ccagtttgat gatgtcttgc aggttagaac ctgcaaccac tttggtcatc
     1141 agcgccagaa cgccgtaagg ggtcagctgc atgaccaggc gaaccagttt catcacccag
     1201 ctttgcaggg tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc
     1261 agcttcagcg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc
     1321 ggattggctc cggtcagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc
     1381 ggaacgctca ggtcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca
     1441 gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc
     1501 acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg
     1561 attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag
     1621 actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac aatgttaaac
     1681 cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcgagg
     1741 ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta
     1801 tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat
     1861 ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt
     1921 gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt
     1981 ccagaaaggt gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac
     2041 tggatctgca aagtattgat catttgcgat gaccaacgaa cagcgctgtt ttcgggtaat
     2101 gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg
     2161 ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg
     2221 ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt
     2281 ccttgtagcg catcagccgg aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc
     2341 agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg
     2401 atcggttttg atatccactt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc
     2461 ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc
     2521 acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga
     2581 ccttccgccg tcatgataaa actcactaaa ctgtcaatac gtacagtata ggatttttat
     2641 acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca
     2701 gttgaatgca aattcagata aagaaccagg agcagtgtga tcactgcccc tggtcattaa
     2761 ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca
     2821 aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct
     2881 ttagaagcga tcatgccatt taccgagtct tccagcgcca cgcaggtcag tgggtcaacg
     2941 cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt
     3001 tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc
     3061 agcatatgta gtggtgacgc ggaggccagt cccactaata aaccttgttc tttgcataac
     3121 gcaacagctt cgcgcacgcc tggtaataat ggacgcgtct cttcaaccag tgaaatggca
     3181 cgagcaataa cccgttctac tacttcctga cggcttggcc cattccaggg ttgccgggcg
     3241 taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga
     3301 cgggagatat ccacccccag gcttgccatc acatccagtt cagctcgatc ccataaaggt
     3361 tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt
     3421 gacattacaa cctctccttt agagcattat gtgtaaagta ttaagccgtg cttatgatta
     3481 agcgcctatc atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag
     3541 tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc
     3601 tgttttgaaa aggattttgg gttgggtgat ttttatacct gcattgatct ctacactgat
     3661 ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta atgcggtcat
     3721 gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct
     3781 gttctggtat aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt
     3841 gattttcatc ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg
     3901 ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg
     3961 ggaagagggg ctaacgcgtg aacaaattga gtcacgtatt gttgtgccgc accatactat
     4021 tttcctgcag tttttctcgc tgtatatcct gccggttatt tgtattgctg caggttatgt
     4081 gttcttttct ctgctggggt ttatttaatc aaaaggccgc acggagcggc cattatcctc
     4141 accccatatt atgctgctaa taatctatcc agtgactgct gagcaataac caaatgctga
     4201 ccgccaaata accttgcacg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca
     4261 aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg tggcggctgt
     4321 tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta gcaggccggg
     4381 tcgaaaatgt acgggccatc cggacccagc gcacagttgc cggaccataa atcgccatgc
     4441 aacagagaag gctgcggttg atgcgaggcc agacgctgct gaatatgctc tacgatggcg
     4501 tcgatattgc cgaaagcgat ccctttctct gctgctagtt ccaactgcca gccaatccgt
     4561 tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgcgg agttgtggag
     4621 agcgagttat caaaatcgag gccaaattgt ggttggtcgc tccattgatg taaacgcgca
     4681 atttgctgac caagaataaa tgcgctatgc gcatccagcg ggcgaggtgg gagataatcc
     4741 atcaccagaa aactgtagtc acggtcagcg ccaactgccc aaaccttagg cacggtgacg
     4801 gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt
     4861 tccctttcat cacatttgac gaaaaagtca cgtcctgcat agcgcaaatg ccatgcggca
     4921 tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg
     4981 ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat
     5041 aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat
     5101 tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggcgatt
     5161 tcaggatctt taccggttgc actttgggta agccccgaaa ccagttctct aatttcttct
     5221 tcactttggg tactgataag tccaaaagtg ttagtcccca gctcatggat atttccatca
     5281 tcatccgtca tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt
     5341 tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg
     5401 gccatagttc acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc
     5461 cgacaggttg gtggtgattc ccccaatgca gggggaatgt ttcagttagc gggaaaggta
     5521 agcgtaaatt ttttgtgtat cgtcatggga gcacaggcgt gttccctgat tgagtgtggc
     5581 tgcactcccc gcagctacgc caaaacggac catctcttca agagaggcat tttctgccag
     5641 tttcagtgtc atcgcgccga ccatgctgtc accagcgcca acggtgctct ggcttttcac
     5701 cggtggtggc acaacctgaa tgcagttttc actatcaacg cccagcgccc cttgtggacc
     5761 cagggaaacg acaactcgtt tggccttgcc gctattaacg atttcctgcg cggctttgcg
     5821 gacatcgtct ggctgggtga gttcgcgatt cactagcgca ctgagttctt tttggttagg
     5881 cttaaccaac tcgatattac caatcgccag tgctgcactt aacgcttcgc cagaactgtc
     5941 gacgatgcag cgaatccctt gtttttgcgc agcggaaatc agttgggtta atttttccag
     6001 cttcacacct ggcggcaggc ttccgcttat gaccaggata gcaccggatt caatttccag
     6061 aacttgctct tcaagctggc gaaactcatc ttcacttaat gccgcgcctg gcatgacaaa
     6121 acgatactgc tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt
     6181 ggcttctaca gtagcgacgg ggacattttc atctgccaac agtgaaacca gatgttcgcc
     6241 ggtcgcacca cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg
     6301 ggcgacgttg atgccgccgc ctccgggttc gaataccggt gcggtacagc gcagctttcc
     6361 ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt
     6421 atagatacgt accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg
     6481 aaagggaaga aattaacaat atgatttatt agagctttat ttaaaatttt gcagataaat
     6541 atatataaat aaaaatctgt tttcttcaca tgcttattca aaaaatgaaa taagaataca
     6601 ttgctaagtt attcaagcct ttttataatt cgttaccttt ccagggacgc taatctttga
     6661 tccttaagaa agtttccggg accgcgatgg tctctttttt tgttgtacgg actcttgata
     6721 aatgaagctt ttgaagacag ttcctgccat cgttatgttg gcggggggca tgtttgcgtc
     6781 attgaatgca gctgccgatg attccgtttt tactgtcatg gacgatcctg ccagcgctaa
     6841 aaaacctttc gaaggtaatc tgaacgcagg atatcttgca caatcaggca atacgaaaag
     6901 ctcctcactt acggctgaca ccaccatgac ctggtatgga caaaccactg cctggtcgct
     6961 gtgggggaac gccagtaata cttcttctaa tgatgagcgt tcttcagaaa aatatgccgc
     7021 gggcgggcgt agccgtttta atttaactga ttatgactat ttatttgggc aggcaagctg
     7081 gctgacagac cgttataacg gctatcgaga gcgcgatgtg ttgaccgcgg gttatggtcg
     7141 tcagtttttg aatggcccgg tacacagttt ccgctttgaa ttcggtccag gtgtgcgtta
     7201 cgacaaatat accgacaatg ccagtgaaac ccagccgctg ggctatgcct ccggggctta
     7261 tgcatggcag ttgactgaca acgctaaatt tacgcagggt gtttcggtct tcggcgcgga
     7321 agacacaacg ctgaactccg aaagcgcatt aaatgttgct attaatgaac attttggact
     7381 gaaggtggct tacaacgtaa catggaactc tgaacctcca gagtctgcac cggaacatac
     7441 cgatcgtcgg acaaccttat cgctgggcta cagcatgtaa aattatctgc cggaccgtgt
     7501 tatgcggtct ggctgtcttc gtttgtcact gctcagcccc aaatcattgc tgcccaacgt
     7561 agcaacaaca tagcgccaca gattgccagt agcaccacca ccatcttcca gtgtttttta
     7621 tcaaagcgtt tagtcggcat accagtgtcc tgcatatctc ttgtgcgtga taacggagtg
     7681 taacaaaagg gacaggcatc aagggatttt ttgttattac agccgtcggg tggcgttgta
     7741 cacctgatca ctgtttttgc ggtttatgaa aaaaataaaa ttatgtatcg atatataaat
     7801 atctggttat a
//
LOCUS       DARNVM010000196         7795 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.196, whole genome shotgun sequence.
ACCESSION   DARNVM010000196 DARNVM010000000
VERSION     DARNVM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.196"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(360..1112)
                     /gene="envY"
                     /locus_tag="SI621_004205"
     CDS             complement(360..1112)
                     /gene="envY"
                     /locus_tag="SI621_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HEI4066853.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQK"
     gene            complement(1295..2185)
                     /locus_tag="SI621_004206"
     CDS             complement(1295..2185)
                     /locus_tag="SI621_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HEI4066854.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVNKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPNAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2186..5158)
                     /gene="nfrA"
                     /locus_tag="SI621_004207"
     CDS             complement(2186..5158)
                     /gene="nfrA"
                     /locus_tag="SI621_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HEI4066855.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDARARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKTRLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLESEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5145..7382)
                     /gene="nrfB"
                     /locus_tag="SI621_004208"
                     /pseudo
     CDS             complement(5145..7382)
                     /gene="nrfB"
                     /locus_tag="SI621_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            complement(7653..>7795)
                     /locus_tag="SI621_004209"
     CDS             complement(7653..>7795)
                     /locus_tag="SI621_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEI4066856.1"
                     /translation="FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
BASE COUNT         1827 a         1998 c         2042 g         1928 t
ORIGIN      
        1 tattgcggtt ttactgtgtg gttttttatg cttttgtggt gcttttatct atttaagtgc
       61 catgccttta gaggcatata agcgaaaata gcatgaggtt tatcctcaat tactatgttt
      121 tttagtacaa aaaagaggga caaaactgag acacataagg cctcacaatg gcttgcaagg
      181 ctttacatgt tttgaggtag tgggacgtgt gagcgcagag atggcgcggt aagttgttga
      241 cttaaaatgt cgttctagga acttctaagt cgtgggccgc aggttcgaat cctgcagggc
      301 gcgccatttc ttcttcattt atccccgtct tatccgtttc cgctttgccc ttcaccacat
      361 cacttttgtc gctgtttggc gagataattc aacggtgtca ggccgtaaaa cgccttaaaa
      421 acagagataa agtacgacgt gctgctatag ccacataatt gcgctacctg agtgatattt
      481 ttgttatcca tcaataacat ctgtacggcg taacgcatac gacactctgt gacaatctgg
      541 ctatagctgg tattttcgtt ttttaatttc tttttgagca ggctggggct taaacatagc
      601 gaactggcga caattcgcag attccagtaa tgctgaatat cgctttgaat aatgcggcag
      661 acgctgtcgc ggacgctgct gcgtaagata tacatcagta gggcaataaa ccgcgattgc
      721 tcaagaaagt tagacaatac ggtaaaaagc aatgcgcgcg tcaactccgc ttcgcagggt
      781 ttgctgggtt gctgcttgct gtggttggcg gctagccgga acacttccgg tgtacggcta
      841 tgacaagcga taaccggggt agccagccgc gaccagggct ttacgcaggt taagttctta
      901 tttaaaaaca acagataatc gttgatgatg ttgcggttga tgtgggtgat taatacgtga
      961 tttaacgttg agagttcaat gacgttattg ttgcacgcga aggcggccag gtaatttttc
     1021 ggaagggtaa acgtggcatg gttattaacg cttacctcta cctctttttc ggtcaggatc
     1081 accacgcaag gttcactgct gctcaattgc atttcgcact cctcagatat cagaaactcc
     1141 gctcaaagga tctatgcttc ctgcatgagt gatcggcccg ttcgccgata acgatcttct
     1201 ttctttagca cgctttttag caattaatct tgatggaatt ctgacgagag cgaaagaggt
     1261 aagccaggtc gtacccgact tacctggagg ggatttaata ctcgagaatg ccgtgtgcga
     1321 cgggcaaata gcgcagagag aaatagagcg tgtctttacc gcaagaggaa cgtttcgcta
     1381 acagcgaggc gatctctgca ttcggtttcg gtttcgctgt aaaggttttg cctttgccgg
     1441 cgacaaacag ttcataaaca atgccgcttg ctggggcgga actctggcaa tcggcgctgg
     1501 cctgtagata acgttcacgc tgttcagcgg taagtttatt cacgccgctg ccatcctgta
     1561 cccagacatt aacgccggtt gctttaacgt gttccagcag ttggcgatag ccatcgggcg
     1621 acatgtttcc ggcgaaaaaa ctactgatat aaaccggttt tgctgaaaca tcgctaatca
     1681 accgctgagc gttgtttaac catgtcagca aaggttgacg ggcggcttcg ctgcgccagt
     1741 tcaggtcgtc aatttccgcg ctgatatacc agccatccgg cgttacgcca ggcgcggcgc
     1801 tccataatct ggcttgctgg agatcggcag ccagcaggcg attaagatag ctttccagcg
     1861 ccgcggatga ctgtttctgg tgcataaaaa attccggatc ggcattcagc ccgacaatca
     1921 gcttcaggcc agcctgttgc gcagctgctg cacgcttaaa caataacgcg cgctgttctg
     1981 gctgggtgaa tgcatcgccg taacgggtcc attgcaaaac cagggtatcg aagccttgca
     2041 aacgtaactg actcatcagc ccctgccact gggtatcggt aacctgacta tcacggtttt
     2101 gtggttgcca gataatacct ttcatcgcaa aggaaaaagg gctgaccaaa agcagtgtca
     2161 gcaatacgaa aatgaactta cgcatttacc agtgcactcc aatggtgaga aacgcgttgt
     2221 tgcgctctcc gttacgttga ttaatcgcct taaaggtatg ttgatattcg acgccgagac
     2281 tgactttgtg cggccaggcg tcgtagtgcg tctcgccggt ccagatattc cagcggaccc
     2341 cgacaccgcc aagctgcgcg ccctgagtgc ctttatcacg atagccgttg tcctgaacgt
     2401 gagcgtaagg ctcaatagtc tgtccgttag ctaccttctg atgccagctt acgcgataat
     2461 ctgccgtcca cgcctgaata tcctggcgga tatattgcgc cgcatcgagg tacaggtttt
     2521 gggcaaacca gcctgaaccg ttcgggtgcc attcgtcgct gtatttgccg ccattaaaga
     2581 atgaggcgct ggcgcgcagc atggtatcgg atgcgccatt ttgcccgttc agcggcaact
     2641 gctgttcgac ggcgaggaaa aagatctgat cgcgcagcgg cttccagcgc agaccggtgc
     2701 cggacatcgg atttttcacc ggcatcatca ccccgttttc tccggtatcg gcaaagacgc
     2761 ggctataaac tgagagcagg tcgccttcca gcagcatatt gcgtccgatg cggtactcgg
     2821 attccagttg cccgtagcta cgatagcttt tccctggcgc tgcgccgccg acattattgt
     2881 tagcggtact catcgcgccg gaacgcaagc cgatggaaga atcgaaactg aacgtccagc
     2941 ggcgaccgac ctcctcatgc agacggcgga aattgaagcg ttgctgattt tgctctgggg
     3001 tcagtggggt tatcagcgcc tgattatcaa tgtcatcaat caccagccgg gcgtagtgct
     3061 gcgtcgcagg catgtcatcc agacgctggt tcacgtaggc cagttgtcgg ataagtgccg
     3121 gatcgtccgg tagcccttta tgcgcctgtt cgagcatttc ccgcgactgt gcgatatcac
     3181 cgctatccca caaggcgtaa ccgagcgctg cctgggtgtt gctattattc ggttccagtt
     3241 ccagcgcggc gcgcaaatca ctcaccgccg ccgggacatt atgacgttgg cgataaattg
     3301 tcgcccgcgc aacgtaagcg ttggcagaag gcgcaatatt gattgagcgc gtgagatcgt
     3361 tcagtgcgag ttccggctga ccaggaatgt aacgttgcgc atgtagccac cagtagaggg
     3421 cattgtttcc cagcccacgt tgttctgcct gctgaagcca gcgatcgcga gccgcaccat
     3481 ttcctgccgc ctgggcggta ttggcagcag caagcagatc ctcattgctc atgtcgtgaa
     3541 gactgatttt ctgccaggcc gccagtgcgg tggcgtagtc ctcaacctga tacgcctgat
     3601 aggctaccgc acgatgttgc caggcgttcg gttgtcgttg ttcggcctga agccatgcat
     3661 acaacgccac accgggtagc gtgtcccgat aacactttgc cagacggttc caggcggcgg
     3721 catcgtagga aggcgacata tcgcccagca agcgaactat tgccgggcaa ttatctgcaa
     3781 tacccggcaa ctgactttgc cactgacgtt gctccgccag cggtaagggt ttcgataaaa
     3841 tcgccacctt cgccggcgtt gccaggtaag gatgactttc cagcagagac gccagtcgcg
     3901 ccattaaagt ctggctgaca cgcgcatcgc cctggaaagg atagcgttgc agcaataaat
     3961 cggcagcttc gcgtgactgc tcgttctgca tcagttgcca ggtcagttga tccaggcggg
     4021 taagatttgc cggttcttgc tgatacagca atcgtgccag acgcagagct tcagccttgt
     4081 tatgggtcgc tacgctaacg gtataacgct cctcaagcat ttcattggcg gggagggtgg
     4141 cgagcagttt ttgcgctgcg tcgtactgac cttcttttaa cagcaccggt agcgtcgcgc
     4201 caacaacata ctggcggtta tcggcaaact gtaccgtata attcgccaac gcctgaacgg
     4261 ggttgccgct gtatttagat aacagataga gccaactttt ctcttgtgcg tccgtggtaa
     4321 atagcggcgt attttcaatg agataatgct ggaggcgtgt tttttcgcca cgataagcca
     4381 gcgcggtcgc gtaagtaata tatgactgag gatcggtgaa gatcccctgt gattgcagcg
     4441 ccaggatccg atcatccagc tgcccggcaa gaagcacgtc aaaccactga cggcgttctg
     4501 ccgcgcttaa tgtgttctgc tggcgtgctt cattgtatag cgtatctgcc tgggaccatt
     4561 gtttcaggta gattgcgcgt tgcagcagat cggttcgcag cgtttttcct tccggcgatg
     4621 cagcaaacgt cgcatcgttc agttgcgctc tggcgacagg taattgtgcc agccgcaggg
     4681 cattctgccc gacttcactg cgacaacgca gggtcggcgc agcatcgcac gctttttgct
     4741 gggcaagcag ttcttcaaca gtcgtaacgc ttttcacttc aactggaata gccgccagac
     4801 tgcgctcaag tcgggcatct cctgggtgac gtttcagttg atcctcaagc aacagccgcg
     4861 cccgggcatc atgaccaaaa tggcgatagg cttcagcaag gtataaagtt agcggaatat
     4921 tatccggcac ctgctggtgt atatattcaa attcgcggat ggcggttgct tcgtcgttat
     4981 ttttctgtgc cttcagcgcc ttgtcgagac ggggataaat aacaaaatgg cgataatcgc
     5041 tcagccccag ctcttctgcg ctggtgccga tattgtctgc gagtgcgctg gtactcaata
     5101 aagacgtcag cagtaaacca gaccatccga tgacgcgatt aaggttattc tccttcattt
     5161 tcggactcca gttgcgcaac ctgttctgtg tttaaacctg ctttgagtaa tagtgattgc
     5221 atcgaaactt gtaattcgcg ttgaattgtc aggacgcgat ccaacgtttc ctggctgata
     5281 acgccttcgg tgaccaaaaa cttgccgagc ggcagagaac tgcgttcatg gcgcaataac
     5341 aacacgttaa ttgctgaacg attaatatga ccgagcgtgg tcagtatttc ggcgaacagg
     5401 aactgatgcg gcacatattg ccgccagatt tcaccggcct gctgttccgt gagccactga
     5461 tgctgaaccg cattgtacaa cattgcccgc ggatcgtgac cgcgtcggcg cgcataccag
     5521 tggcgtaacc ccgtgacaat ttgtccccgc agaacaatga cgtaacgcac tttgcgtccg
     5581 actttacgcg tcagggccgc cagcgaaacc gggtcaatac catcttcact gccgacaatt
     5641 aactcatcat tttccagacg cagcggcagt actgcataat gcagcgccac ggaagccggc
     5701 atttcggcaa tcagtgatga agggatctgc caggcgtcga tcgactccca cgccacgccg
     5761 ttttgctctg ccagcgcctg tgccagctgt tcggcgctaa tcagcccctg catcagcatt
     5821 gaaccgccca ggcgtagacc ttcgacgcga ttacgcagtg ctgtatcgag ttgttcttca
     5881 gtgatgacct gattttccag caggatttga cctaacggac gcaacgagcg ggtatcgcca
     5941 gtcacgctgg ggaagtcatg cgttgtttta tcccacgcga cacgacgtgg atcgccgtgt
     6001 tgaagtacct gttttaacgc gcgccagttg gccatgaagt taatcaggtt gccccagaaa
     6061 agacgcagga cagatagcag cccctgcgtc aggccgtagt agccagtaac gaaaatcacc
     6121 cgctgcacga tgcggttaat catcaagcca aagtttagcc acagcagagt cattaaccat
     6181 gcgctgccgc tgaaaataga aaggaaatgc caggcatcgg gccacaaact ttcatacgcc
     6241 agcaacagca aaagctggat catcaccagc atcgcgagga agctgacaaa gttactgatt
     6301 gcccctttgc ggtcgcgcca gagaaagtag ttcagcgtca gactggaggt ccatttgtgg
     6361 gttttaaagc cctagaaaac aatgccgatg atccagcgag atttttgtcg aaccgcagtc
     6421 gaaaaggtat cggggaaata ttcgcgcacg cagatcatgt ttgacgtccg cgcgtgctgt
     6481 aaaaatttac gctgctcgcg ttctttggct tcgtccacca ccggaaaacg gacaaaaatt
     6541 tccgtcatac ctttttcttt caggcggaag ccaatgtcgt aatcttcagt aagactctgc
     6601 acgtcgaaag caataccgtc accgtcagct aacagtgcgg tcacggcgcg gcggctgaaa
     6661 caggtaccga cgcctgcgct gggcacttgt ccggcgaggg cttcacgcac cggaacatct
     6721 ttgccatgca gttctgaaaa ctcatcaatg taagtcatgc tggtgaagtg cgtccattcg
     6781 cgttcgaacg gatacaccgg gatctgaatc agatctttac gctcgaccag atagttgaac
     6841 agacgcaatt ccatcggtga aatcacatct tcggcgtcat gcagaataaa accagcaaaa
     6901 gcgaaattgg cgctacgctc aaactgggtg atggcgtcca gcacgttgtt caggcagtcg
     6961 gctttgctgg tggggccagg tcgcgcgcag actaccttat gcacattcgg gaagcgagcg
     7021 cacacttcgt caacatcacg ctgagtatcg gggtcgttgg ggtaggtgcc aacaaagata
     7081 tgatagtttt catagtcgag cgtggtcgcc gccagctcgg ccatattgcc gatgacgccc
     7141 gtttcattcc acgccggaac cataatcgct aacggttttt catctggttt atacagttcg
     7201 cggtaactca ttcgcgggta gcggcgataa acactcaact tgcgtttaat gcggcgtacc
     7261 cagtagacga catcaataaa aaaatcgtcc agcccgctga tgaacatgat gaccgctaac
     7321 gttatcgcga ttacttttaa gccgtagagc caggtagcaa aaacatcaag aagccagtcc
     7381 acacaaaaac cttacattaa cgctggttat gtttagggtg gcgtatatta aggtttttta
     7441 tgaattgtga cagcttttta ccattaatag gtatgactat tgcggcacgt tatttttaca
     7501 ctggttataa aagttgccgt ttgcgtagaa tgtagacaaa agcatggtgt cctgggacag
     7561 ggaggttaac gtcatagtga aaaggagcgg tggcaccgag caggtcatca tgtagtggaa
     7621 gtgcctggag gaggtgagca ggcagggcaa gattacgaaa gcccgctccc cgcaaggact
     7681 gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg
     7741 aataccttat cattcgtcaa aatattaata gcgatgtgcc gtatccctga aaata
//
LOCUS       DARNVM010000197         7663 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.197, whole genome shotgun sequence.
ACCESSION   DARNVM010000197 DARNVM010000000
VERSION     DARNVM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.197"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..124)
                     /locus_tag="SI621_004210"
     CDS             complement(<1..124)
                     /locus_tag="SI621_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HEI4066857.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFK"
     gene            complement(389..1123)
                     /gene="cysH"
                     /locus_tag="SI621_004211"
     CDS             complement(389..1123)
                     /gene="cysH"
                     /locus_tag="SI621_004211"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEI4066858.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRTNLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1197..2909)
                     /gene="cysI"
                     /locus_tag="SI621_004212"
     CDS             complement(1197..2909)
                     /gene="cysI"
                     /locus_tag="SI621_004212"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEI4066859.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2909..4708)
                     /gene="cysJ"
                     /locus_tag="SI621_004213"
     CDS             complement(2909..4708)
                     /gene="cysJ"
                     /locus_tag="SI621_004213"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HEI4066860.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVNVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5027..5389
                     /gene="queD"
                     /locus_tag="SI621_004214"
     CDS             5027..5389
                     /gene="queD"
                     /locus_tag="SI621_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEI4066861.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5467..6738
                     /locus_tag="SI621_004215"
     CDS             5467..6738
                     /locus_tag="SI621_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEI4066862.1"
                     /translation="MEDNCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6729..6989
                     /locus_tag="SI621_004216"
     CDS             6729..6989
                     /locus_tag="SI621_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEI4066863.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7006..7581
                     /locus_tag="SI621_004217"
     CDS             7006..7581
                     /locus_tag="SI621_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEI4066864.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
BASE COUNT         1674 a         2062 c         2023 g         1904 t
ORIGIN      
        1 ccttaaactc cataccacgt tctctgatac tcaactcaca caacgagtcg cctaccatca
       61 gcgtgaatcc cagtactgta caacacagta cgatgattgc cacaagggca tattttgtca
      121 gcatgtcttg cctccctgca aagaagagac taacattcga attgcttagg ttcgaacgct
      181 ggcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc
      241 ttaacgccag acagcctcaa gcacccaacg ccattctata cctgataatt cttccagcgg
      301 cgttcacgtt taaaatcagg aatgctgctc gcaaaataaa aacgccccca tcatttctga
      361 cagaggcgtt taatttatcc ggcaatattt acccttcgtg taacccacat tctcttttta
      421 agccaaagaa acgcgtttct tcttccgcca tgccgggttc ccatttacgg gttgtatggg
      481 tatcaccgac cgataaatat ccttcatccc ataatgggtg atatttcagg ccatgttttt
      541 gcaggtactg ataaatagtt cggttatccc agtcgataat cggcagcact ttaaatacgc
      601 cacgctgaat tgccagcacc ggtaaattgg tgcggctgcc ggattgttca cggcgcaggc
      661 cagcaaacca ggtttgcgca ttcagttctt tcagcgcccg gttcatcggt tcgactttgt
      721 tgatgtcatt gtacttttca atgccttcaa caccctgctc ccacagtttt ccgtaacgtg
      781 cttcctgcca ggcggcgctt tcggtagcgc ggtacacttt caggttgagc ttgagtttgt
      841 ccgttaactc gtcaataaag cggtaggttt ccgggaacaa gtaacccgta tcggtgagga
      901 tcactggaat atccgggcga atctgattca ccagatgcag gctcaccgcc gcctgaatgc
      961 cgaaactgga agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc
     1021 cttcagcgtc cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta
     1081 ccttcggcag ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg
     1141 ccttgcctga tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa
     1201 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
     1261 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
     1321 gacgccagga tttccggttc ggtgatgttt tctttataca tccgtgggat acgtgtccca
     1381 atgcggttgc cgccaagatg caggttgtag cgacccggcg ctttacccac caggcccact
     1441 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
     1501 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
     1561 aaacgctcgg cttcggccat cgccagcggg caagtcggga atgacacgca ggccattgag
     1621 ttttcacgct gcggcgtgac ggcattcatt aacccgctct ctttggcaat cttctcgatc
     1681 ttcgctttct cgctttccgg cacaccggcg atgatcagat tctggttcgc cgtaatgcgg
     1741 aaatcgcctt tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgcccagga
     1801 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgcct
     1861 ttaacccagc caatacgatc gccgcgtccg gtgaactcat atggacggat cggttcaaat
     1921 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc
     1981 gtgtatttgg ttttggcatt tttacgatcg gtacggttac cccagtcgcg ctgagtcgtc
     2041 acgacggctt ctgccaccgc cagcgtatgc tccagcggca gatagccaaa ctcactcgcc
     2101 gtgcgggcgt aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccaacagg
     2161 ttaaagccca ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc
     2221 agatcgatat cgttctgcgg cgggatcact accgtagttt tgaatttacg cggcaaataa
     2281 gtctgaccga ggatcggttc ttcatcggtg gtggcgactt tttcctgatc gagccagatc
     2341 tccgcatacg cgcgggtacg cggcagcaga tgttcagaga ttttcttcgc ccattcatac
     2401 gcttccgcgt gtagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
     2461 tcgttagcag tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
     2521 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
     2581 ccatagatgg tgttttcacc ggcaaattta tcgatcgcct gccactgttt ggtggtaatc
     2641 acgccacccg gcagacggca gcgcaacagc attgcatgac gtggctccag cttctgttca
     2701 gcacgttcgg cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
     2761 aggaagttat cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
     2821 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
     2881 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
     2941 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
     3001 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
     3061 aaatgtgggc accgtcattg atccagcgcc acagctctgc gccctgttcg cgcagtttgt
     3121 cttgtacgta aattttttct ttttgatcgc gcgaccaggc aagatcgata cgcgtcagca
     3181 cgccctcttt gacgtagcgc tgccattcca cctggtagag gaaatcttcc gtaaagtgcg
     3241 ggttgccaaa gaacagccag tttttccccg gcgcttcgtc ggcggcgcgt tgctgcataa
     3301 aggcgcggaa cggcgcgatg ccggtgcctg ggccaatcat aatcaccggc gtttgcgggt
     3361 tagcgggcag gcggaagtta tcgttatgtt cgataaagac gcggacttca ccctcttctt
     3421 ccacgcgatc cgcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt
     3481 aacgcaccac gccaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcga
     3541 tggaatacag gcgcggggtc agcgggcgca gcaggttaat cagcgcttcg gcatccagtt
     3601 gcgccggaga gaaacgcacc atgtcgacaa tcggcgtcgt cgcggcgtaa tgctgtaact
     3661 tcgctttatc gcccaccagc ggcagcagcg tttcgctgcg ggtaagcgtg gcatagttct
     3721 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagcg
     3781 gcagggtttt gccctcaacg ttgacaggtt catcaccttt cagccacagc agttcgacaa
     3841 gttctttcac cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt
     3901 aacgcaggcc cgagtcacct aagtcaattt cgatatggcg aacgtctttt tcagagttac
     3961 gcccggtaat tttctggtta acagagaggc tcgccaccag cggcgcgtct ttgctgtacg
     4021 ggctggtgtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
     4081 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgatggca gcagcctggt
     4141 attcaacgtc ggcatcgaca cggtcgagca ggcgttcacc gcccagttcc gccagcttgc
     4201 tgtcaaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
     4261 cggcaaatgc ggtgttttcc agcttcggcg cttttttgga gaacaggaac ttatgcagcg
     4321 cgacggcttc ttccggcggt tccccttctc cttgcgttga cgtcaccacg ataagcagtt
     4381 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
     4441 ttgccgccaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
     4501 aagcggagat aatagttata cccggcattt ctgcggcggg cgctggcgtc gctgcaagcg
     4561 cagctggctg ctggttgagc acgccccaga aatagccaga aacccaggca agctgggtgg
     4621 gagttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
     4681 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
     4741 ctgcgccctg tcagcgccca taaaacagaa gagaagataa ggttaacggg gtgaacggtg
     4801 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
     4861 gtctttaaca acaaaataga ttaaccaacc caatgaaaaa caaatgaata ttgcccatca
     4921 ataagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
     4981 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
     5041 atttaaagat ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa
     5101 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
     5161 tccgcatacg ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaaccta
     5221 cgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
     5281 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
     5341 gatggtaaaa gaaacctgta ctgcaggctg tatctatcgc ggcgaatgat aagagtgtgt
     5401 cggcggtcaa tttcccttaa gtaacgccat gttagggtgt tgtgttctgg atatctgggg
     5461 catgacatgg aagacaactg cgacattatt attattggtg ccggtatagc aggtaccgct
     5521 tgtgctttac gctgcgcgcg ggcaggtttg tccgttctgt tactggaacg cgctgaaatc
     5581 cccggcagca aaaatctttc cggcgggcgt ttatacaccc atgcgctcgc ggaactcctc
     5641 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttctctg
     5701 ctaacgccgg atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt
     5761 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa
     5821 tgcatccccg gtgcgacggt ggatgcgctg tatgaagaaa acggcagggt gtgtggtgtc
     5881 atttgtggtg acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc
     5941 gtcctggctg aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg
     6001 atcaaagaag tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat
     6061 aacgaaggcg cagcgttgct gttcagcggc gggatctgtg atgacttacc cggcggcgca
     6121 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
     6181 acgcaaagtc gtgttccggc aagcgagctg ctggctcgct ttaaaacgca tccggcagtg
     6241 cgcccgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt
     6301 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctgct gggcgatgcg
     6361 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctaactggc
     6421 gcgcaggcgg cggcacaaac gctgataagc gcctgccagc accgcgagcc gcaaaatctg
     6481 tttccgcttt atcatcacaa cgtcgagcgc agcctgctat gggatgttct acaacgttat
     6541 cagcatgttc cggcgctttt gcaacgccct ggctggtacc gtgcgtggcc tgcgttaatg
     6601 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
     6661 ttattctggc gtcatttacg tcgtcacggc ctgtggcatc tggcgggcga tgttatcagg
     6721 agtctgcgat gtctgtagcc cgtaatctct ggcgcgttgc cgatgcgccg cacattgttc
     6781 cggctgactc cgttgagcgc cagacggtac aacggttgat taacgcttgt ccggctggtc
     6841 ttttttcgct cacaccggaa ggtgacttac gcgttgatta tcgcggctgc ctggagtgtg
     6901 gcacctgccg tttgctgtgc gacgaatcaa cactacaaca gtggcgctat ccgccttccg
     6961 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
     7021 ctgctccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggca
     7081 atcgattctg aatgccagtt tatttcagtg ttgtacggca atatctgcac catcagtaat
     7141 atcgttaaaa agattaaaaa cgccggaaaa tatgccttta ttcatgttga tttgctggaa
     7201 ggcgcatcga acaaagaagt ggtgattcag tttctgaaac tggtgaccga ggcggacggc
     7261 attatcagca cgaaagcatc gatgctgaaa gcggcaagag cggaaggttt tttctgtatt
     7321 catcgcctgt ttattgttga ttcgatttcg tttcacaaca ttgataagca agttgcgcaa
     7381 tcgaatccgg attgtattga gatcctgcca ggctgtatgc ctaaagtgct gggctgggtg
     7441 acggagaaaa tccgccaacc gctgattgcc ggcgggctgg tgtgcgatga agaagatgcg
     7501 cgtaatgcga ttaacgcggg tgttgtggcg ctttccacca cgaataccgg ggtctggacg
     7561 ttagcgaaaa aattgctttg acgggatact cgtaacgagt tgaatttgtg tgatttgtag
     7621 gctgcacgcc acatccgaca ttcagcgcct gatgcgacgc ttg
//
LOCUS       DARNVM010000198         7646 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.198, whole genome shotgun sequence.
ACCESSION   DARNVM010000198 DARNVM010000000
VERSION     DARNVM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.198"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..486)
                     /locus_tag="SI621_004218"
     CDS             complement(<1..486)
                     /locus_tag="SI621_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HEI4066865.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMISGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LT"
     gene            756..956
                     /gene="glgS"
                     /locus_tag="SI621_004219"
     CDS             756..956
                     /gene="glgS"
                     /locus_tag="SI621_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HEI4066866.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            complement(991..1290)
                     /gene="ubiK"
                     /locus_tag="SI621_004220"
     CDS             complement(991..1290)
                     /gene="ubiK"
                     /locus_tag="SI621_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HEI4066867.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     regulatory      1412..1560
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1664..2317
                     /gene="ribB"
                     /locus_tag="SI621_004221"
     CDS             1664..2317
                     /gene="ribB"
                     /locus_tag="SI621_004221"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEI4066868.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRIMTVRAAIADDAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            2579..2749
                     /locus_tag="SI621_004222"
     CDS             2579..2749
                     /locus_tag="SI621_004222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066869.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(2807..3580)
                     /gene="zupT"
                     /locus_tag="SI621_004223"
     CDS             complement(2807..3580)
                     /gene="zupT"
                     /locus_tag="SI621_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEI4066870.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            3723..4511
                     /gene="ygiD"
                     /locus_tag="SI621_004224"
     CDS             3723..4511
                     /gene="ygiD"
                     /locus_tag="SI621_004224"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEI4066871.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVE
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(4557..5228)
                     /gene="dsbI"
                     /locus_tag="SI621_004225"
     CDS             complement(4557..5228)
                     /gene="dsbI"
                     /locus_tag="SI621_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HEI4066872.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRFLWLLMAIAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(5243..5911)
                     /locus_tag="SI621_004226"
     CDS             complement(5243..5911)
                     /locus_tag="SI621_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEI4066873.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYEVAKIQGVPAYVVNGKYLIYTKSIKSIDSMADLIRE
                     LASK"
     gene            complement(5929..>7646)
                     /locus_tag="SI621_004227"
     CDS             complement(5929..>7646)
                     /locus_tag="SI621_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEI4066874.1"
                     /translation="AGFKPAPPAGQLGAVIVDPYGNAPLTALVDLDSHVISDVKVTVH
                     GKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANKVTVEWKENGKVMKDDYVVHTSA
                     IVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTHTFTAQGSDLHWHGEKDKNAGIL
                     DAGPATGALPFDIAPFTFIVDTKGEYRWWLDQDTFYDGRDRDINKRGYLMGIRETPRG
                     TFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGKSNYRRDDG
                     VHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVDLAHAGQQA
                     KLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRDKQVKWILA
                     PSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFTYTQHTAWISSKGTLTIFD
                     NGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPITSIIEYQA
                     DRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEIDVLSDKPNQTHYRALLVRP
                     QQMFK"
BASE COUNT         1960 a         1771 c         1885 g         2030 t
ORIGIN      
        1 ggtgagtttt ccttcgcagg gattacctga agtggcctga tgaccggtaa ttaacgccat
       61 gctaccaata tattcttctt ctgtaatagc tgaactatgg tcgcgtggaa tccagggcgc
      121 aactatttta ccggtataat gcaccgcaat aatcgtaaac agtaaactga caggaaccac
      181 gaacaggttc gaaagtggtg attgccagac catgaggcag gcatgctgca acaggatgcc
      241 aataagacca aaaaaaccag ccagtaagca aagaacgacg agagctggca atcttccgat
      301 attgagataa tgaagtgcct ggctaatatg acctgtggtt atggaatcat aatggtcaag
      361 atgtgcatca agtgcaccgg agatcatgtg accgcagata agcgcaaaaa tttctaaaag
      421 gccgatcaac agtacaaagg aaatagcgaa aagataaggg gtgttatagt cggcgaataa
      481 aatcatatgt cactccaatg tccatttagt gacgcaatat ttactttggg atagtttcct
      541 tgcttacatt tatctttgtt aataataaca catgttagat aagttctgga agagaaagtc
      601 ttttaatatg gttttttatt ttttcactaa aaagtgtgat cggggacaat atatttacgc
      661 acgttatgtt taaaggcact acactgattg gggaatactg aaatcagaaa aagcatatgg
      721 ccttaaaagg tatgcgataa ccggaggatg tgcttatgga tcatagtctt aattctttaa
      781 ataatttcga tttcctggcg cgtagttttg ccagaatgca cgcagaaggt cgcccggtcg
      841 atattctggc cgttactggt aacatggatg aagaacatag aacctggttt tgcgcacgtt
      901 atgcctggta ttgccaacag atgatgcaaa ccagagaact tgaattagaa cactgatatt
      961 acgggccgtc tggcccgttt ttgtgttgct ttattctgct ttatcaatag gcgggatagc
     1021 cggaggagat tgaagatctg ctaccgaatt attgcgcgct tccagttcac tgatgcgctg
     1081 ctccagtaac gccagttttt cacgagtccg taacaggact tgcgtctgaa cgtcgaattc
     1141 ttcgcggctt accagatcga gacgcgtcag ctgcgcttgt agggtttggc ggattttttt
     1201 ctccacatct tccccgaact ccctgattcc tttaggcatt gattcgtgaa cctggcgagc
     1261 gatttgctca atttttttcg ggtcaatcat tgtagtttcc ctgtactgat aggtgttgag
     1321 tgccattgta gtgcgataag ggtaagtcat aaaccagaat tatgtgaagc tatgcgttgc
     1381 tgccgctaat cattagcgtt atagtgaatc cgcttattct cagggcgggg cgaaattccc
     1441 caccggcggt aaatcaactc agttgaaagc ccgcgagcgc tttgggtgcg aactcaaagg
     1501 acagcagatc cggtgtaatt ccggggccga cggttagagt ccggatggga gagagtaacg
     1561 attctgtcgg gcatggaccc gctcacgtta ttttggctat atgccgccac tcctaagact
     1621 gccctgattc tggtaaccat aattttagtg aggttttttt accatgaatc agacgctact
     1681 ttcctctttt ggtacgcctt tcgaacgtgt tgaaaatgca ctggctgcgc tgcgtgaagg
     1741 acgcggtgta atggtgcttg atgatgaaga ccgtgaaaac gaaggcgata tgatcttccc
     1801 ggcagaaacc atgaccgttg agcagatggc gctgaccatt cgccacggta gcggtattgt
     1861 ttgcctgtgc attactgaag atcgccgtaa acaacttgat ctgccaatga tggtagaaaa
     1921 taacaccagc gcctatggca ccggttttac cgtgaccatt gaagcggctg aaggtgtgac
     1981 taccggtgtt tctgccgctg accgtattat gaccgttcgc gcagcgattg ccgatgacgc
     2041 aaaaccttct gatctgaatc gcccgggcca cgttttccca cttcgcgctc aggctggtgg
     2101 tgtactgacg cgtggcggcc atactgaagc gactattgat ctgatgacgc tggcaggctt
     2161 taaaccggct ggtgtgctgt gtgagctgac taatgacgat ggcacgatgg cgcgtgcacc
     2221 agagtgcatt gagtttgcca ataaacacaa tatggcgctc gtgactattg aagacctggt
     2281 ggcttatcgt caggcacatg agcgtaaagc cagctgaaaa ccgctgctta atttactgcc
     2341 ttaatcaaga aaccgaagtt gtagcaggct tcggttttta tttttccctg ctatgcaaga
     2401 tgttaattcc gcttctcttt ctatgagaaa atttcattaa tatcaggcat tctttttcat
     2461 tatgatcaaa atcatataat tgttccgtta ttatgcgacc atatgaatat atccgaaaaa
     2521 ggatatgtga aatttatgcg ctaaatgttt agtattgtta aatattaagg gtaaaaggat
     2581 gtttatcgcc tggtactgga ttgtattgat tgctctggtt gtggtgggtt atttcctgca
     2641 tttgaaacgt tattgtcggg cgtttcgcca ggacagagac gcactgcttg aagcgcggaa
     2701 caaatactta aacagtacga gagaagagac agccgaaaag gtagaataag gctttcgtga
     2761 agctggcaga agttgtccat gcgctggaca acttctgatg ttgtttttaa ccaattcccg
     2821 ccgtttgtag cagcactaaa ctgaatccca tcactgacat tccacatagt acgccatagc
     2881 tggggttatt attagggtca atctctttgg cgagcggcat taattcatca accgagagcg
     2941 ccaccataat tcctgcaacc gccgccatga ttgccgccat gaccaccggg gaaatcatgc
     3001 taccgaggat taaccacgcc agcacaccac caagaatttc tgccagtcca gaaatccccg
     3061 cccaaagaat cgcggtgcgt ttagaccccg ttgccgcgta aaccgggcct gcaaccgcca
     3121 gaccttcagg aatattgtgt aacgcaacgg ccagtgcgat gccaaagccc agctccaggt
     3181 tgctgcttgc tgtaacaaag gtggcaatcc cttccgggaa gttatgcaga ctgataccga
     3241 gagtgagcag aatggctgtg cgcttgatcg attttggcaa cggctgcacc gatttttgca
     3301 ttaaatcctg cggatgcgca tgtggcaaca tgcggtccag gccaaaatag ccaagcagac
     3361 cgaagataaa cattccataa cccaacacag gcgacattcc ttcagctgct agtgcggcag
     3421 gaagcatttc cattaatgag atgagcaaca tgatccccgc cgcaaatcct agcgaaaacg
     3481 ccagtaagcg gttcgagggt ttttgcccga gaacgccgag aaacgcgcca ataaacgtgg
     3541 ctgcccccgc cagtatggtc agaatgagag gtactgacat cgacaactcc ttataaatct
     3601 tcaccttcag gcagatcgtc aaaataactg atgatcatca tcattgttat ccgggttctt
     3661 actccgccga acaagcgtat tgtgaggata tcaaaatgac acctttagtt aaggatatca
     3721 tcatgtcttc aacacgtatg ccagcattgt ttttaggtca cggtagtccg atgaacgtgc
     3781 tggaagataa tttgtatacc cgcagctggc agaagttggg gatgacattg ccacgcccgc
     3841 aagcgattgt ggtggtttcg gctcactggt ttacccgtgg aacaggagtg accgcgatgg
     3901 agacgccgcc cacgattcat gactttggtg gcttcccgca ggcgctgtac gacacgcatt
     3961 atcctgctcc gggttcgcct gcgctggcac agcatctggt tgagctgtta gcgccggtcc
     4021 cggtggagct ggataaagaa gcctggggct ttgaccacgg ttcctgggga gtgctgatta
     4081 agatgtaccc tgacgccgat atcccgatgg tgcagttgag tatcgacagt agcaaacctg
     4141 ccgcctggca tttcgaaatg gggcgcaaac tcgcggcgct gcgtgatgaa gggataatgt
     4201 tggtcgccag tggcaacgtg gtgcataacc tgcgtacagt gaagtggcac ggtgatagtt
     4261 caccgtatcc gtgggcgacg tcgtttaatg agtatgtgaa agcgaatctg acgtggcaag
     4321 ggccagtgga acaacatcct ctggtgaatt atctcgacca tgaaggtggc gcgttatcga
     4381 acccaacgcc agagcactat ctgccgttgt tgtatgtctt aggggcgtgg gatgggcagg
     4441 agccaattac cattcctgtc gatggtatag aaatgggcag tttaagtatg ctgtcggtgc
     4501 agataggtta ataaaagaac ccgaagccag aacgactggc ttcgggtgtt gctgagttag
     4561 cctctgatga ttttaagcgc ccatgcgcca ctcataatga ccagcagcac cagacacata
     4621 ccaaaagcca gcatacaagc ctgagccata ttcataaaat gccatggtgg tagcagatac
     4681 cagccttcag attgctgata catttccaca aaccactgtt gcgtgctgct cagtgtgacg
     4741 ccatcaggaa cgattggtgc gtcatagcca caatccccgg taggcttgaa ccaatttggt
     4801 gcccactgtg ccagcggcag gttaaaggga aaagttggat cggtagagca accctgtacg
     4861 ccaaacagtg aatcgggatc cggattatgt actgcatggt ggatatcgtt cagtttgagc
     4921 gaaaacttca gtcccaaaat gctgccgtaa aatgccatca cgcagccaat tagctttagg
     4981 atgatgttct ttgggttgat cgctgcaacc aatcctccaa taaccatcac aaacatggcg
     5041 tagcgaatgt atacacattg ctcacagggt gccatgtaga gatagatctg gaagaaagag
     5101 tgcgccagga taattaacgc gcccatcgca atggccatta acaaccacaa aaaacgttgt
     5161 tcttgccatc ttaccagcgt gtccaccggt gaagtgcgaa ggtctttcca cattccctta
     5221 atacccatag cctgtttccc tgttatttgc ttgctaactc gcggataagg tctgccattg
     5281 agtcaataga tttaatactc ttggtgtaga tcaggtattt accattgacg acatacgccg
     5341 gaacgccctg gatttttgca acttcataag aggctttcca tttttccagc gtttcctgaa
     5401 ctgcaggctc tttcagcgcg gcttcaaaat cggcctgact catacctgcg gcgtccagtc
     5461 cggttttgat aaaggcagcc ggatctttac catcagacca gcgttctttt ttgtcatgat
     5521 aagccgcgta ataagcaaac ttggctttct taaactgaga gttagcatca aataatgaaa
     5581 tacctgctgc tttatcttta ttgatcaaga ccgcaaagac ttcgcttgct tgtttgccgt
     5641 attcaccttt tgtttccaga tggaatggag taaaagcaac gatatctttc actttttccg
     5701 ataccggacc ggtaaccgct ttgtcgtact tgtaacagaa cgggcaggcg tagctaaata
     5761 ccttaatcag cgttttgtcg gcattcggaa taggtttttc cagaaccatg taatccgtgc
     5821 cttcggtaaa tgcagaagcg gtaaatgaaa cagccaaagc tgcggtaagc agtatggttt
     5881 taaatagtga tgagatccct aatttcgaca tttttattcc cttcttaatt atttgaacat
     5941 ctgttgtgga cggactaaca gtgcacgata gtgagtctga ttgggtttat ctgacagcac
     6001 gtcgatttcc actttcactt ctttggtttt gtaatcgatt tcgttcaact taccgacggt
     6061 tggctgcccg acatcgaaca aatgaataga accaccgaag ccaaacatgg tgttacggtc
     6121 ggcttgatat tcaatgatgg aggtaatcgg gctatagaaa tcgtagccac gttctttacc
     6181 gtattcccac acttgctgaa cggtgccttt cttctcatca atcttatatt ccacaaagcg
     6241 ggagtatttc atggttggta aggcaggttg ttccagatga cgcccatcgc cattatcaaa
     6301 aatggtgagc gttcctttgc tggaaatcca ggcggtatgc tgggtgtagg taaagtcgaa
     6361 gtctgagttt tcgcacaggc cattttcgtt acaggtaatt ggcttaccgt tagcatcaac
     6421 cggtttcagc agcttgctgg ccagcggttt ttcccaacct ttagagggcg caaggatcca
     6481 tttcacttgc ttatcacgac caattttcac aacaccctgg tggcgagagg agaggatgat
     6541 tgagtcatct tttgcgtcat aggcgattga gttaacgtgc gcccagttac gacctggccc
     6601 tacacccagc gcgtcgccga acggtgtatc tggttccagt tttgcctgtt gtcctgcatg
     6661 ggcaaggtca acgttaacgc aaactgcacc tgcatccagc gcgccgagca gtgcatcgcg
     6721 tttcggatcg aggatcttgg tcagatccca tacatcgaca acgcgaccag atttatcgac
     6781 ttcgagaata tggtcacgaa tggtggtgac gtgtacgccg tcatcgcgac ggtaattgct
     6841 cttacctacg cgcaacaata ctgtgccatt aggcgtctca atggactcat gagtggcgtc
     6901 agcaaatccg cgcggtagtt tgtgatcttc gagcacctgc cccatcatgt cgaactcgta
     6961 ccagtgctga ccttgtacag cggtaaaggt gccgcgtggc gtttcgcgga tacccatcag
     7021 ataaccacgc ttgttgatat cgcggtcacg accatcgtag aaagtgtctt gatccagcca
     7081 ccagcggtat tcacctttgg tatcgacgat gaaagtaaag ggcgcgatat caaaaggtag
     7141 tgcgccggtt gccggacccg catcaaggat accggcattt ttatctttct caccatgcca
     7201 gtggagatcg gaaccttggg cggtaaaggt gtgggtatta accagataga ggcgatcttc
     7261 aaaacccggt gcgactttaa taactttggt ctgttgtaaa tcggagatag agcggttatc
     7321 catgtaatta ttgacgatgg ccgaagtgtg caccacataa tcatctttca tgaccttgcc
     7381 gttttctttc cactcaacgg tcactttgtt agcaaatttc tgataaagac caaaaatcgg
     7441 tacaccatca taagttttta gtgattcctg acccacggga tagctgattt ctacgccttt
     7501 ttcgcccttc ccatggacgg taactttgac atcagaaatg acatggctat ctaagtcaac
     7561 caaagcggtc agtggtgcat tgccgtaggg atcgacaatg accgcaccca gttgcccggc
     7621 aggtggtgcg ggtttaaaac ctgctg
//
LOCUS       DARNVM010000199         7585 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.199, whole genome shotgun sequence.
ACCESSION   DARNVM010000199 DARNVM010000000
VERSION     DARNVM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.199"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            422..2707
                     /gene="nrdA"
                     /locus_tag="SI621_004228"
     CDS             422..2707
                     /gene="nrdA"
                     /locus_tag="SI621_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEI4066875.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2796..3926
                     /gene="nrdB"
                     /locus_tag="SI621_004229"
     CDS             2796..3926
                     /gene="nrdB"
                     /locus_tag="SI621_004229"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEI4066876.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            3926..4180
                     /gene="yfaE"
                     /locus_tag="SI621_004230"
     CDS             3926..4180
                     /gene="yfaE"
                     /locus_tag="SI621_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HEI4066877.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4234..4884)
                     /gene="inaA"
                     /locus_tag="SI621_004231"
     CDS             complement(4234..4884)
                     /gene="inaA"
                     /locus_tag="SI621_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HEI4066878.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4965..6155)
                     /locus_tag="SI621_004232"
     CDS             complement(4965..6155)
                     /locus_tag="SI621_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4066879.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLLVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNLAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6307..7185
                     /locus_tag="SI621_004233"
                     /pseudo
     CDS             6307..7185
                     /locus_tag="SI621_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         2041 a         1950 c         1765 g         1829 t
ORIGIN      
        1 cgacatcaac aatcgcttac attcgcttat atattgacca taactgatac atcagattat
       61 gtgatgactc gtgcttagat caatttttgc aatcattagc caaaagatta ataaaccatc
      121 tatatcaatt tatctaacct attcttccgt tcaagaaatc gccgaacagt tatttttaac
      181 aaatttttct cttcccattg actttcccgg acaccttgtc tgacctaagg tgcgcgaaag
      241 ccactttttc cttcctgagt tatccacaaa gttatgcact tgcaagaggg ccattttcac
      301 actatcttgc agtgaatccc aaacataccc cctatatata gtgttctaag cagcttccct
      361 tactacaggt agtctgcatg aaactattgc ggaaagaatt ccaaaaacag gtacgacata
      421 catgaatcag aatctgctgg tgacaaagcg cgacggtagc acagagcgca tcaatctcga
      481 caaaatccat cgcgttctgg attgggcggc agaaggactg cataacgttt cgatttccca
      541 ggtcgaactg cgctcccaca ttcagtttta tgacggtatc aagacctctg acatccacga
      601 aaccattatc aaggctgccg cagacctgat ctcccgtgat gcgccggatt atcagtatct
      661 cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc tacggccagt ttgagccgcc
      721 tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc aaatacgata atcatctgct
      781 ggaagactac acggaagaag agttcaagca gatggacacg tttatcgatc acgaccgtga
      841 tatgaccttc tcttatgctg ccgttaagca gctggaaggc aaatatctgg tacagaaccg
      901 cgtgaccggc gaaatctacg aaagcgccca gttcctttat attctggttg ccgcgtgcct
      961 gttctcgaac tacccgcgtg aaacgcgcct gcaatacgtg aagcgttttt acgacgcggt
     1021 ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg
     1081 tcagttcagc tcctgcgtac tgatcgagtg cggcgacagc ctggattcca tcaacgccac
     1141 ctccagcgcg attgtgaaat atgtttccca gcgtgccggg atcggcatca atgccgggcg
     1201 tattcgtgcg ctgggtagcc cgattcgcgg tggcgaagcg ttccataccg gttgtattcc
     1261 gttctataag catttccaga cagcggtgaa atcctgctct cagggcggtg tgcgtggcgg
     1321 tgcggctacg ctgttctacc cgatgtggca tctggaagtg gaaagcctgt tggtgttgaa
     1381 aaacaaccgt ggggtggaag gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa
     1441 caaactgatg tatacccgtc tgctgaaagg tgaagatatc accctgttca gcccgtccga
     1501 cgtaccgggg ctgtacgacg cgttcttcgc cgatcaggaa gagtttgaac gcctgtacac
     1561 caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc
     1621 gctgatgatg caggaacgtg cgtctaccgg tcgaatctat attcagaacg ttgaccactg
     1681 caatacccat agcccgtttg atccggccat cgcgccagtg cgccagtcta acctgtgcct
     1741 ggaaatcgcc ctgccgacca aaccgctgaa tgatgtcaac gacgagaacg gtgaaatcgc
     1801 gctgtgtacg ttgtctgctt tcaacctggg cgcaattaat agcctggatg aactggaaga
     1861 gctggcgatt ctggcagtac gcgcactcga cgcgctgctg gattatcagg attacccgat
     1921 cccggccgcc aaacgtggag cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt
     1981 cgcttactac ctggcgaagc acggtaaacg ctactccgac ggcagcgcca acaacctgac
     2041 acataaaacc ttcgaagcta ttcagtatta cctgctgaaa gcctctaatg agctggcgaa
     2101 agagcaaggc gcgtgcccgt ggtttaacga aaccacttac gcgaaaggga tcctgccgat
     2161 cgatacctat aagaaagatc tggatgccat cgctaatgag ccgctgcatt acgactggga
     2221 agcactgcgt gagtcaatca aaacgcacgg cctgcgtaac tccacgcttt ctgctctgat
     2281 gccgtcagag acttcttcgc agatctctaa cgccactaac ggtattgaac cgccgcgcgg
     2341 ttacgtcagc atcaaagcgt cgaaagacgg tattctccgc caggtggtgc cggactacga
     2401 gcatctgcac gacgcctatg agctgctgtg ggaaatgccg ggtaacgatg gctacctgca
     2461 actggtgggt atcatgcaga aatttatcga tcagtcgatc tctgctaaca ccaactacga
     2521 tccgtcacgc ttcccgtcag gaaaagtgcc gatgcagcaa ctgctgaaag acctgctcac
     2581 cgcctacaaa ttcggggtca aaacgctgta ttatcagaac acccgtgacg gtgctgaaga
     2641 cgcacaagac gatctggtgc cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgcaa
     2701 gatctgataa tcccccagcc tttgcaccaa accgtcccct ctccccttgc gggagagggt
     2761 tagggtgagg ggtaataaaa ccacaggaca cattcatggc atataccacc ttttcacaga
     2821 cgaaaaatga tcagctcaaa gaaccgatgt tctttggtca gccggtcaac gtggctcgct
     2881 acgatcagca aaaatatgac atcttcgaaa agctgatcga aaagcagctc tctttcttct
     2941 ggcgtccgga agaagttgac gtttctcgtg accgtatcga ttaccaggca ctgcctgaac
     3001 acgaaaaaca catctttatc agcaacctga aatatcagac gctgctggac tccattcagg
     3061 gtcgcagccc gaacgtggca ctgctgccgc tgatctcgat ccctgaactg gaaacctggg
     3121 tcgaaacctg ggcgttctca gaaaccattc actctcgttc ttatacccat atcattcgta
     3181 atatcgttaa cgatccgtcc gttgtgtttg atgatatcgt caccaacgag cagatccaga
     3241 aacgtgcgga agggatctcc agttattacg atgagctgat cgaaatgacc agctactggc
     3301 atctgctggg cgaaggtacc cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg
     3361 agctgaagaa aaaactgtat ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct
     3421 tctacgtcag ctttgcttgt tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg
     3481 ccaaaattat tcgcctgatt gcccgcgacg aagccctgca cctgaccggt actcagcata
     3541 tgctgaatct gctgcgcagc ggtgcggatg atccggagat ggcggaaatt gccgaagaat
     3601 gtaagcagga gtgctatgac ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg
     3661 attatctgtt ccgcgacggt tcgatgattg gtctgaataa agacattctc tgccagtacg
     3721 ttgaatacat caccaatatc cgcatgcagg cagtcggttt ggatctgccg ttccagacgc
     3781 gctccaaccc gatcccgtgg atcaatacct ggctggtgtc tgataacgtg caggttgctc
     3841 cgcaggaagt ggaagtcagt tcttatctgg tcgggcagat tgactcggaa gtggacaccg
     3901 acgatttgag taacttccag ctctgatggc tcgcgttacc ctgcgcatca ctggcacaca
     3961 actgctgtgc caggatgaac acccttccct tctggcggcg ctggaatccc acaatgtggc
     4021 ggttgagtac cagtgccgcg aaggttactg cggttcctgt cgcacgcgcc ttgttgcagg
     4081 ccaggtggac tggattgccg aaccgttagc ctttattcag ccgggggaaa ttttgccctg
     4141 ttgttgccgg gcaaaaggcg atattgaaat cgagttgtga tcgggtacta gcgccagata
     4201 cgacgcttac gcgtattatc tggcctacaa cgattacatg gcgtagtaat acgctttcac
     4261 ctgctcccag tccgctttgg ggatcggctc cagatatttt tccagctggc ggaagtcatg
     4321 attaatcgct ttatcgcgac gcaagcggcg acgacttttc tccagatcaa gaaatccagc
     4381 ttctgcctta ccttctgttt tcacatagat atggcgaaca tagcaacagc catgctgacg
     4441 attaacgcta tgcattttct taaacgccag cgccactgct ttcaacatgg cttgccgtac
     4501 ttcgtcagaa tagggcgtta ctgcatgctg ggcataccag tcagcaatgc tgatgaaccc
     4561 cgccatatct tcagtcacca gcaacgcgcg ccattccccc tcaattttca ccgcttcacc
     4621 aaaaacgatc ttcggcacga tgacaccggc ccgttccagt tctttaatta ccgcaacctc
     4681 acggacaatc gttggtcggc cgaacggata acgtacggaa tgaaacagat gatgcgtcat
     4741 gcgctttaca tatagctttt tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt
     4801 gcggcgatag ttaggctctt caacccagtc gccctctgtt gcccaccagt gattaaattc
     4861 gtcgtgcttt gctgaaactg ccatacccta tcgcctgtca ttttttatta acgacaatga
     4921 ctataggtgg ttacctgcga aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt
     4981 gccactacta gcgcaacacc gcttttaccc ttcatttgca gcgcatagac tccaccagcc
     5041 actaccacta atagtgcacc cagcagataa atcattcggt agccagtatg tgcaaccatc
     5101 atccccatca cacaggctcc cagcgccagg cctgcatcca tcgcattgaa gaagagggag
     5161 tttgcgacac cgattttatg cggttccact gaactgataa tctgcgtctg aaaaactggc
     5221 gtcacggaac catacccgat accaatcaat gcgccagaaa caaccatcat tatgcttccc
     5281 tgggtgtagc cgagaaccac cagtcctacc gtaaagaaca caaggcaagg gtaaaccaca
     5341 tatttcggtc cttttttgtc gcagacattg ccggtaaagg tacggcagat catcaggaag
     5401 atagcgtagc aaagcaggaa attactggct gccgacatca ggttaagttc gcgtgcataa
     5461 agcgccagaa aagcagaaac cccggcatac gaaaaggtca taaaaaaggt gaccatcgca
     5521 aatggcaacg ccgcgcgatc gaacatggcg gcgaagccca ttttcggcgt tgaaccatca
     5581 gcatggctga taaccggcgg cactgtcata atcagcgaca gaataatccc caccgctgcc
     5641 acgccggtgc atagccagaa agccagatta aaggcttccc agcgcgccat attcagacca
     5701 atccacggcc cgaccaccat tgccagcccc attgccagag agaaaaaact gatgccctcc
     5761 ccacgtcggg aggccgggat cagccgcgcg gaaatcgttc cttttaccgt agtgataacc
     5821 ccgaaggtaa taccatgaag cacccgaata aaaagcagtg attcaattga gtggcaaaaa
     5881 ggatacaacg ctgtgaccaa taaaaaggcc agagaagaga gcaccagaat agttttattc
     5941 gagtatttac ccacccattg cccggcaaat gggcgaataa caatggcggc aattaaaaat
     6001 aaagtcacca ataaaccggc tttatcagga gaggcatgca agcggtcaga gatataaatt
     6061 ggcaacaatg ttagtaaaac ataaaagacg aagaaaataa taaagctgat gatggttatt
     6121 gcccaaaaat ccttcgtcca taacttctct ttcataccct accgcctgtc aggatgacgc
     6181 acgcagacaa cactcccgtt actgcggttg cgtcaaattg tgatgtactt tgcagaacag
     6241 aagtataaat ggctgcatgc tattagagaa attaatcgtt ttaatattat tgattagcaa
     6301 actctaatga ctcttaccca aataaactct ttactcgccg tgctggatta cggtggcttt
     6361 actgaagcca gcaagcgata gttcatgacg cagtcggcgg taagtcaggc aattgctagc
     6421 ctcgaacaag aacttggtgt gaatatactg ttacgcgatc gccgtaaagc tatacaactg
     6481 accacagcag gtcatcgcat tgtgcaacat ctgcgcgcca ttaatcggga agttaacgca
     6541 gtaaaagaga tagccgagca ggaaaagcag aaccctcaaa gaacgctgag acttggttgt
     6601 ttccccagcg tctgcgcctg tatcttaccg gttgtgattc gctactttga aattcatcat
     6661 ccgaatataa aaattattcc ttttgaggaa aatagcacag cgattattga ttcgcttcag
     6721 aatgaatcca ttgatgccgg ttttgttcat tttccggtga gcggaatgta ctccgtgccc
     6781 atttatcagg ataaattcac cgtagtgtta ccgcccgacc atgctctggc aaaaaacagc
     6841 actattacag tggaagagtt aatgggtgaa ccattaatta ttagtaaagg acgctatgaa
     6901 ttaagcatta tggcactatt taaagaaaag aatattaccc cacagataaa atatgaattt
     6961 aatcacccgg atacagcgat cagttttatt cgacagggat taggcatcgc cttattacca
     7021 gagttaacgc tgaaaaccat tgcagatgaa ttgtgttccg ttcctctcga accaaccttc
     7081 tatcgacaga tttcgttgct ggctaaagaa aagccggtag aaggcagtcc actgttttta
     7141 ctacaaatgt gcacagaaca attagtggtg agtggaaaaa tttgatgtaa gacatgagat
     7201 tgatgacaaa tgcaagactg cctgattcgc cacacttagt tgtactatag cctttaccag
     7261 gcgtaataat tcgttttcac ctgattttgg cccactttgt gaattggcga tatatattta
     7321 tccagttggg agaagtgaca atgactccag gtagaaatcg gtatgaagcg cccgactcac
     7381 tgtcaaaacc gatcgttgag agtaatgaca ctccggcgaa cagtacacag tttactctgg
     7441 agccattctc aatcttcaag tgggatacct agtaactttt cagggaagtt atcaatatct
     7501 gtttgttact tattaccgta tttattaaca ataatgatac tttctaccat gcgtaaaggt
     7561 cttcgtaata aaatgagcta aattt
//
LOCUS       DARNVM010000200         7573 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.200, whole genome shotgun sequence.
ACCESSION   DARNVM010000200 DARNVM010000000
VERSION     DARNVM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.200"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            272..1021
                     /locus_tag="SI621_004234"
     CDS             272..1021
                     /locus_tag="SI621_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HEI4066880.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     FSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            948..3070
                     /locus_tag="SI621_004235"
                     /pseudo
     CDS             948..3070
                     /locus_tag="SI621_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3240..4292)
                     /locus_tag="SI621_004236"
     CDS             complement(3240..4292)
                     /locus_tag="SI621_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEI4066881.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQNKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDMPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYMAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVAVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4607..5923
                     /gene="shiA"
                     /locus_tag="SI621_004237"
     CDS             4607..5923
                     /gene="shiA"
                     /locus_tag="SI621_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HEI4066882.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            6025..7479
                     /gene="amn"
                     /locus_tag="SI621_004238"
     CDS             6025..7479
                     /gene="amn"
                     /locus_tag="SI621_004238"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEI4066883.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKYQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2028 a         1780 c         1724 g         2041 t
ORIGIN      
        1 attctgctgc aatcaatgca tacataacac aaatatcact caggtacact actcaaacca
       61 cgctgggatt tttcctcaag ttataatcat caccccgaaa atcattcggc atttactcat
      121 taaatagtca ccccataggc ctgtacatgt ttactcagaa atatacatcc ttttctctgt
      181 cataaaccct ctgattaatc ataaataaac acttgtgaca ccaatctttt tccttaacgg
      241 aacgaattgt tgtgtagaag gagataatat tatgtcaaaa aaatatcagc cattgcttat
      301 aactcattat atgtcaacat gggtcactat aacggaagca gttgagatca ctaccaaagc
      361 cataaaacaa aaaattactc ctagcgatat ttatcgtcat gcctttagtg gcaacatcct
      421 actatcggtc tatttccagt ctcctgtgat acttaagaag atacaaactt ttaatggaaa
      481 aataaaattc aggcagtttg tgggagacct acttgataaa ctatgcatgc ttgacaggga
      541 cggttttatt tatggtcaaa atttaagact ctgtacagaa gccagatata tttgtcctgt
      601 gcaacaaatt atagataccc ccctgttacg caaactcaac cagtttcgta cgtttgttcg
      661 taatgtccgc ccgggtgatg aactggatgt ccaggcacaa gttagtgaaa aaaatttaac
      721 cccgccgccg ggtaacagca gtggcaacct tgagcaacag atagccagta cttcacagct
      781 aattggatct ctgctcgccg aagatatgaa cagcgagcaa gcggcaaaca tagcacgtgg
      841 atgggcctct tctcaggctt caggggtaat gacagactgg ttaagccgct tcggtaccgc
      901 aagaatcacg ctgggcgtgg atgaagattt cagcctgaag aactcccgtg acggcaaccc
      961 tcgaaaatgg cgattcgatg caacaaacag tgaactatgt gccgaacgtc acgaatgctg
     1021 aaatcacgct ggcagcctcg aaggatccgg tgattgccga caataacgat ctcacgacac
     1081 taacagcacc gtcgctgata cagagggcaa tgcgatagcc aacactgagg taacatttac
     1141 tctaccggaa gatgtgaagg cgaacttcac gctgagcgat ggcgacaaag cggttactga
     1201 tgccgatggc aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc ataccgtgac
     1261 agcatcgatg gttggcggta agagtgagca gttggtggtg aactttactg cggatacgct
     1321 cactgcgcag gttaatctta acgttaccga ggacaatttc atcgccaata acattggaat
     1381 gaccaggcta caggcaacag tgactgatgg aaacggcaac ccagtagaag gtataaaagt
     1441 taatttccgt ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcaggt
     1501 tttcgctgaa attcttgtga caagcaccga ggtcggacta aaaacagttt cagcctctct
     1561 ggcagataaa cctactgaag tcatctcgcg attactgaat gccaaggtag atgttaattc
     1621 tgcgacgatt accagtcagg agatacctga aggtcaggtc atggtcgcac aagacatagc
     1681 agttaaagct cacgttaacg accagtttgg caacccggtg acgcatcaac ccgcgacatt
     1741 cagtgcagcg ccatcctcgc aaatgatcat cagccagaat acggtctcta ctaatacgca
     1801 gggagtagcc gaggtcacca tgacgcctga aagaaacggc tcgtatactg taaaagcatc
     1861 cctagcgaat ggagcttcac ttgagaaaca actggaggct attgatgaaa aactgacact
     1921 cacgtccagt ccgcttatcg gtgtcaatgc tcctaaaggc gctactctga cggcaacgct
     1981 aacctctgca aatggcactc cagtggaggg tcaggtcatc aactttagcg taacgctaga
     2041 aggtgcgacg ttaagtggcg gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt
     2101 tctgaccagc aataaagtcg gtacatatac ggtgactgca tcgttccata acggcgtcac
     2161 aatacagaca cagacaaccg tgaaggttac tggcaaccca agcaccgcac atgttgctag
     2221 ctttatcgct gatccgtcga ctatcgccgc caccaacagt gatttaagta ccttaaaggc
     2281 aacggttgag gatggcagtg gtaacctgat cgaaggtctc actgtgtact tcgccttaaa
     2341 aagcggctcc accacattaa cgtcattaac agcagtgacc gatcaaaacg gaatcgcgac
     2401 aacaagcgtg aaaggagcga taacggggag cgtcactgta agcacagtca caagcgcagg
     2461 tggaatgcaa acagtagata tatcgctagt ggcagtccca gcagacgcct cgcagtccat
     2521 ccttaagaac aaccagtcat cactgaaagg ggactttacc gatagtgctg agctacatct
     2581 tgttctgcac gatatatcag gcaatccgat caaagtttct gaagggatgg aatttgtgca
     2641 atcaggtaca aacgtgccct atatgaaaat tagcgcaatt gattacagtc aaaatatcaa
     2701 cggtgattac aaagctattg ttacaggaga cggagagggt atcgcaacgc tgatccccgt
     2761 attgaatggt gttcatcagg cgggcctgag taccacgata gaattcatta gcgcggaaac
     2821 cagaccaatg accggtacag tgtcagtgaa tggtgcaaac ctaccgacag cttcattccc
     2881 ttcgcaggga tttaccgggg cgtattatca gttgaataat gacaactttg caccaggaaa
     2941 aacggcggct gattattcgt tttcaagctc ggcctcctgg gtaggcgttg atgctactgg
     3001 taaagtgaca ttcaaaaatg acggtgatag caatactgta ataatcacag cgcccccaag
     3061 atccggtggt gctatttacc aaacagttcc tcctgaatcg cgttcggtgt gactatgatc
     3121 attttcggtg agtattgctt tgatccaaag gctggttttc tgtgatgatt gaccagcctc
     3181 agagtattga tggcaccacc gttcataaca gatcggcatt actatgcata agtactgcct
     3241 caaattctcg ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca
     3301 attttttgcc caacggcgac tgactccgaa acatcggcca ttgcgggcca tcgcgttttg
     3361 taatcagcat aaattgcaac aacaggggta ttataggcgc ttgccatatg taatattgag
     3421 gtgtcaggag ttattgccat ataagcatct tttataattg ctgctgaacg tttaatcgag
     3481 ggtgataagg ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct
     3541 tcccctttgg gtccataaac gatcactatt ggcatatctg tttcactttg aatttttgct
     3601 attagatttt ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg
     3661 agcgcaatgt aagatcctaa cgatcgcatt tcctcgcgta cctcatcgag tacatcatcg
     3721 cttaatggca attcatatat tgacctgact gccggaaaac ccgcctcacg catcataaat
     3781 gcccatgtca tgggtacagg agcccggaga ttctgatcca tccgggacgc gttcttacac
     3841 aaaggggaat agcacttcat ggttaaaccg acgacctgga aattcgtttt tgctttcaac
     3901 tgactgataa atatcatcgt tttcaggttc tttttacgca tcgcctctat acaaagatcc
     3961 ggcgtaccgt attgcagtgt tatgttcttc gctaaatctt ttatttcttt taatgttgcg
     4021 tgatcctgca tagtcattgt gactaatgtt aatttattct gttcaagttt gagcgcatta
     4081 aagacttcta aattaattgt cgacgtaaca attaatagat gcttaatttt atgcaattca
     4141 agcgcccgaa taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatgtatg
     4201 acaacaaagt ctgttttttc aatattaaaa ttataagctt tataatcgta gtaactaaat
     4261 gcaatacgtc tcaacaatga tactaaaaac ataactaacc tcgcctccct actggttata
     4321 atgcaatgca gtctatcaga atcatcaggg tgcaattttg tgcatatgcg gacttttatg
     4381 tttcatatct cgaacctgtg ggccctctgc ttaatcctta aacaacacca gcaactcctg
     4441 cgctttcatc ttccatcgaa tttttcatgt tgccgttaat cagccataaa atcatttgca
     4501 gattcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
     4561 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
     4621 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
     4681 aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc
     4741 cgcactggtg tttaatcgcg agtttttccc gcaggtaagc ccggcgatgg gaacgctcgc
     4801 cgcatttgcc acctttggtg tcggcttcct tttccgaccg ctcggcggtg tcattttcgg
     4861 tcactttggt gaccggctgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
     4921 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
     4981 tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
     5041 cgcagcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt acagtagcgg
     5101 tgtacaagtt ggctacggag taggtttact gctttcaacc ggactggttt cattgatcag
     5161 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
     5221 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     5281 tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct
     5341 gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac
     5401 gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
     5461 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     5521 ttttgcctgg cttgccgatc gttttggtcg gcgcagggtt tatatcacag gcgcgttgat
     5581 cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat
     5641 agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca
     5701 accgatgttt accgaaatgt ttggtgccag ttatcgctac agtggtgctg gagtcggtta
     5761 tcaggttgcc agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac
     5821 ttactttgcc gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc
     5881 tgcaatgacc gctttattga tgaaagacaa tcaacgctcg tgatagccta gcgaagatca
     5941 tccgatcttc gccttatact tttgtttcac attcctgtga catactatcg gatgtgcggt
     6001 aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag
     6061 gcactggata aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt
     6121 ggcaaatata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt
     6181 gtctatccat cactgaccgt aacctgggac ggtagcacaa ctaatccccc caaaacgcgc
     6241 gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc
     6301 tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc
     6361 tctgtgcaac cctcgcagca tgaaatccct tatccttatg tcattgatgg ctctgaattg
     6421 acacttgatc gctcaatgag cgctggttta actcgctact tcccgacaac agaactggcg
     6481 caaatcggcg atgaaactgc agacggcatt tatcatccaa cggaattctc cccgttatcg
     6541 cattttgatg ctcgccgggt tgatttttcc ctcgcacggt tgcgccatta taccggtacg
     6601 ccagtagaac attttcaacc gttcgtcttg tttaccaact acacacgtta tgtggatgaa
     6661 ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct
     6721 tgtgctggcg ggaactggat cactgccgaa accgaagcgc cagaagaagc catttccgac
     6781 cttgcatgga aaaaatacca gatgccagca tggcatttaa ttaccgctga tggtcagggt
     6841 attactctgg tgaatattgg cgtgggaccg tccaatgcta aaaccatctg cgatcatctg
     6901 gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagc
     6961 caggccattg gagattatgt ccttgcacac gcttatttac gcgatgacca cgttcttgat
     7021 gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat
     7081 gacgctacca agttagtcag cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca
     7141 ggtactgtgg taaccacaga tgacaggaac tgggagttac gttactcagc ttctgcactt
     7201 cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa
     7261 ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttacat
     7321 ggtgagatta agcttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacac
     7381 ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattca
     7441 cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttatgaa ccttcttatc
     7501 agaaatagat gtgaaggatt aagtaagaca tctggcaaat agcctgcaat aaacgcgggc
     7561 tgtttgcttc ctt
//
LOCUS       DARNVM010000201         7548 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.201, whole genome shotgun sequence.
ACCESSION   DARNVM010000201 DARNVM010000000
VERSION     DARNVM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.201"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(71..2116)
                     /gene="dcp"
                     /locus_tag="SI621_004239"
     CDS             complement(71..2116)
                     /gene="dcp"
                     /locus_tag="SI621_004239"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HEI4066884.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWMRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAIIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDNFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2253..2999
                     /gene="ydfG"
                     /locus_tag="SI621_004240"
     CDS             2253..2999
                     /gene="ydfG"
                     /locus_tag="SI621_004240"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HEI4066885.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3088..3774
                     /gene="ydfH"
                     /locus_tag="SI621_004241"
     CDS             3088..3774
                     /gene="ydfH"
                     /locus_tag="SI621_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HEI4066886.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            3951..4154
                     /gene="ydfZ"
                     /locus_tag="SI621_004242"
     CDS             3951..4154
                     /gene="ydfZ"
                     /locus_tag="SI621_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HEI4066887.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4190..5650)
                     /locus_tag="SI621_004243"
     CDS             complement(4190..5650)
                     /locus_tag="SI621_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEI4066888.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATKHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5740..7023)
                     /gene="ydfJ"
                     /locus_tag="SI621_004244"
                     /pseudo
     CDS             complement(5740..7023)
                     /gene="ydfJ"
                     /locus_tag="SI621_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         1969 a         1944 c         1727 g         1908 t
ORIGIN      
        1 cccagctgct gcaattacta ctctaaccag gctggcctgg cgatttctcc aggccagcca
       61 ttggtggtgt ttatacgttc aagccacgat gttgcagcat cggcataatc tgaggtgcct
      121 taccgcgcca ttgtcgatac aggcgttcca gatcctcgct gttacctctg gaaaggatcg
      181 cctcgcgaaa acgcagccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
      241 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgcg gcatatccgc
      301 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagcg ggaagatcca
      361 tattttccgc caccagcgcc cgcaattcga aattatcgac atcctgcatt gcttcgtttt
      421 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
      481 atcctttatt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
      541 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
      601 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
      661 cataacgctg gcgggcaaag aggccgtgta gcgtatgacc aaattcatgg aataaggtta
      721 tgacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
      781 cgttataaat taccggatgc gtttcattca gcgttgattg ctcaacaaaa ttgcccatcc
      841 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccaa
      901 cgccattatg atcaaaaatt tcccacacac gaacgtcagg atggtagaca ggaatatcaa
      961 aacgttcgac aaacttaata ccgaagagct gattcgcagt ccagaatacg ccttcattca
     1021 acaccgtgtt taattcgaaa taaggcttga gctgcgcttc atcgagatcg aatttctcac
     1081 gtcttacctg ctcggcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     1141 gcttatcaat aatcgcctgt atggaggcta actcatcgct cgcacgttga cgcgccgctg
     1201 gaacaatttc ccgcataaag ttgagcgctg cttctggcgt ttttgccatc tgatcggcga
     1261 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgcgcg cggatctcca
     1321 ccagacgttg aatgatagcg cgggtatcat tggcatcatt tttttccgct cgcatccagc
     1381 ccgcagtgaa cagtttttca cgcgtcgcgc gatcgcgcag ttcagcaagc gctggttgct
     1441 gggtggtatt cagcaacgga atcagccatt tgttatccag acctttctcg cgagccgcct
     1501 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgaaa tcgttcacaa
     1561 ccagaccgcc ggatttattt gctgccagta accgctggtt aaactggctg gtcagggtcg
     1621 cagcttctgt attcagtact tttaattttg ctttatcagc ttgtgcaagt ttggctccgg
     1681 caaggacaaa acgttgatga atgacctcca ccaggcggat ggattcacta tcaagtccca
     1741 gggattcacg gcgttgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     1801 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     1861 cattagtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     1921 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgcttgcggg ttaagcgcga
     1981 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     2041 gattggcaat ttgatcaaaa tggggagcca gatacggcag tgtgctttgc acaaggaaag
     2101 gattcattgt tgtcattttc ttctcctgaa cgcgaggtgt tccatagcgt aggcttactg
     2161 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     2221 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     2281 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     2341 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctttata
     2401 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     2461 ccgagtggag caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     2521 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aataaaggcc
     2581 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     2641 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     2701 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     2761 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     2821 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     2881 aagatgtcag cgaagccgtc tggtgggtat caacgctgcc tgctcacgtt aatatcaata
     2941 ccctggaaat gatgccggtt acccaaagct atgccggact aaatgtccac cgtcagtaat
     3001 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     3061 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     3121 cctgtcaatc agcagattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     3181 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     3241 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     3301 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca acggcagttt tatccgtcag
     3361 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     3421 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     3481 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     3541 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     3601 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgagatttt ctctgccctg
     3661 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     3721 gaatccgtgc gccagatccg ccaggaaaac agcgactggt ttagcgaaga gtaattcatt
     3781 tcctctcatc ccattcgggg tgagagtctc tccccccgcc ttatggctca tgcatgcatc
     3841 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     3901 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaacacct atgacgacct
     3961 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     4021 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     4081 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     4141 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     4201 aatatttcgc aacggtcgct ttcgcaccat gcgctaataa agacaagtac gcttccgtca
     4261 cttttgcagt aaacaagcta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     4321 gcaatgactg gacgcgcgct ttcccttcgg cactactttg tacagccttc tgaataacag
     4381 gtaacagtgg gtcactgatt tctatcggat ttccctgttc atcaacacca ccgacataac
     4441 gcatccaacc tgcgacgccc agcgccagca gatcgaactt gctgtcatgc gccagatgcc
     4501 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     4561 aggttcgatg acgtagcgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     4621 gcaaatcaac gccctgcact ttcaacgtcg gcgcttgttc ctgcaacatc aaggcatacg
     4681 ctgcatgacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccggcaagat
     4741 accccagata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     4801 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattccgga cgtccggcaa
     4861 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgcca gcaggatcgc
     4921 gcacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     4981 ggtccaccat tgttgatggg aaagtcacgt ttacttcgat ccattgtgcc agttttacat
     5041 caacagcttg cgcgtaggaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     5101 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcga gccagcgcct
     5161 caacaatcac ccctgttgca gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     5221 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     5281 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     5341 catctatctg tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc gtccacgcat
     5401 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     5461 gttcgccgcc gattaaattg acctcataat atccccaatc actgaaatgt tttgtagcaa
     5521 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc gccaaagcct aaatgaacaa
     5581 ttcttggggc caagttatta cgatcataaa cagggagcgt cgcttttgct gataacaaat
     5641 tatttcccat aacaattcct taaatttaaa tatggcaagc tgcatgtttt gttatatgaa
     5701 taaaaaatcc cctctccggt aagagaaggg atggaggatt tacagacttc ttgaaggttg
     5761 cgcagctgtt acaacacgcg gttgatcttc cgcggcatct tccagtgcac ttaaatcacg
     5821 gtctctcact tctggcattt tcagcgcaga gattaaacca atcactgaat atgccatgat
     5881 cataatggcg atcggatacc aggattccgt catggtgcag aaaatacccg ccaggatagg
     5941 accaaaaccg gaagcgataa gaccaccaat ttctttagaa atcgccatcc gggtaaagcg
     6001 gtttttacag ccgaacattt ctgccatggt aatgttttcc agagcaaata atcccagcac
     6061 cgcacagtta tgaatcacaa tcagtgcaac cataatggtg ctcggggcat agcttttatc
     6121 tacgatgata gaaagcattg gccacgccag cacaatcgca gaggtattca taataatata
     6181 cgggatccgg cgacctattt tatcggataa ccaaccaagg aacggaatgg tcataaagcc
     6241 gagaatcgaa ctgatcatca atgcatctgt tggaattgct ttgttaaaca ataacgtctg
     6301 cactaaatag cctgcaagga aagtctgaat taaaccggag ttaccagcct gaccaaaacg
     6361 cagccctgtt gccagccaga aggatttgct ctggaacatg ctaccagcag gtgcaggttt
     6421 tgctgtcggt tggttgctgt cgttaacctt ctcaaagacc gggctttctt tcagattcat
     6481 acgcaaccag atagcaaaga ccatcacgac aacgctcgcc aggaacggta tacgccatcc
     6541 ccacgcaagc agttcctctt tactgagaat gaagaacata aaggcccaga ttgccgttgc
     6601 gctcaaggtt ccgcagttag tccccatagc aacaaatgag gagataattc cgcgcttacc
     6661 ttttggtgca tattccgcca gcatcgtgcc ggcaccggaa atttccgcgc ctgcccccag
     6721 cccctgaata atacgcagcg tcaccagcaa aattggcgca aacacaccaa tttgtgcata
     6781 ggtcggcagc acaccaatta aggtagtaca gatccccatc atagtgatgg taataaagag
     6841 cactttttta cgcccgattc tgtcgcccat tttgccgaaa ataaatgctc cgacaatacg
     6901 cgccacataa cctgcaccgt aggttcccat tgccagaatt aacgccattg ccgttgatga
     6961 ttcaggaaaa aatatttcat gaaacactaa cgctgcgccg agcgaatata actggaaatc
     7021 cataattcac aggtgttttt tcccatcctg tggtttcctt ggcgttttct aggttttttc
     7081 agatagttgc attttttaaa aagcatccta agttcgatct cagtgtctat ctggggccta
     7141 tttctgtccc atatatgccc caaaaaaact cctcaacaga taagtagttt tttcatggag
     7201 ttacgcgtaa aatcaataac ggctggaaat cattcaatac tcgcactatc gaaaaattta
     7261 ccagccaacc gcagcacgtt cttgcataag gcgtgtctgc ggtttttcaa ctattcagat
     7321 acatcactcc catcacattc attcctccgc atcaaaggca tataggctat atcaccttga
     7381 tatttttctt cttcagataa aaactgttat ctatgtatac ttttaaaccc aatccgtgta
     7441 gagtctctgc ataagatagt ttgcagttgc cacttcagct tgcgccataa accgcctgat
     7501 ttttgccgcc atctgttagc attcctgtat acctgaaacg acaatgtt
//
LOCUS       DARNVM010000202         7429 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.202, whole genome shotgun sequence.
ACCESSION   DARNVM010000202 DARNVM010000000
VERSION     DARNVM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.202"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            243..473
                     /gene="chaB"
                     /locus_tag="SI621_004245"
     CDS             243..473
                     /gene="chaB"
                     /locus_tag="SI621_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HEI4066889.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            631..1326
                     /gene="chaC"
                     /locus_tag="SI621_004246"
     CDS             631..1326
                     /gene="chaC"
                     /locus_tag="SI621_004246"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HEI4066890.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1370..1723)
                     /gene="ychN"
                     /locus_tag="SI621_004247"
     CDS             complement(1370..1723)
                     /gene="ychN"
                     /locus_tag="SI621_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HEI4066891.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1909..3303
                     /gene="ychO"
                     /locus_tag="SI621_004248"
     CDS             1909..3303
                     /gene="ychO"
                     /locus_tag="SI621_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HEI4066892.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3304..3954)
                     /gene="narL"
                     /locus_tag="SI621_004249"
     CDS             complement(3304..3954)
                     /gene="narL"
                     /locus_tag="SI621_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HEI4066893.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDISVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3947..5743)
                     /gene="narX"
                     /locus_tag="SI621_004250"
     CDS             complement(3947..5743)
                     /gene="narX"
                     /locus_tag="SI621_004250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HEI4066894.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAEGDGQLAQLQGL
                     QDYWRNELIPTLMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVLVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            6082..>7429
                     /gene="narK"
                     /locus_tag="SI621_004251"
     CDS             6082..>7429
                     /gene="narK"
                     /locus_tag="SI621_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HEI4066895.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIAC"
BASE COUNT         1738 a         1814 c         1966 g         1911 t
ORIGIN      
        1 atcgaaaatg taaaaaatag gtaggaaaaa taacagaaag tgtctggata tcggtaacat
       61 tttacgaatt tttaccccgc tgtcgatttt ttactcatcg ggccataaaa ataagcaatg
      121 cgtttagaca atgtttgttt tagcgtttct taagaagagt ctgacatgaa aattcttatg
      181 ttttggcaag agtggatatt gttgaccaca cttaatgttc aactttgtaa aaggagtcaa
      241 cgatgccgta taaaacgaaa agcgatctgc cggaaagcgt aaagcacgtg ctaccgtctc
      301 atgctcagga tatctataaa gaagcgttca acagcgcatg ggatcaatat aaagataaag
      361 aagatcggcg tgatgacgcc agtcgcgaag aaaccgcgca taaagtggcc tgggctgctg
      421 tgaagcatga atatgccaaa ggggatgatg ataaatggca taaaaaatcg taaaaccggt
      481 agcttagtta aagcaattcg tgcggtgttg ccttgcaagt ggtccgtgga ttgcatattg
      541 tcccgttagt ggtttcaaaa tgagcagtaa aaatgtccgg aagacaccaa aaagttgtcg
      601 cagggaagta tgcagtggcg gaggtgtaag gtgataacgc gtgatttctt gatgaatgcc
      661 gattgtaaaa cggcatttgg tgccattgaa gaatcactct tatggtcagc agagcaacgg
      721 gcggcttcgc tggcggcgac gctggcttgt cgacctgatg agggaccggt gtggatcttc
      781 ggttatggat cgttgatgtg gaatccggca ctggagttta ccgagtcgtg caccggtaca
      841 ctggttggat ggcatcgtgc gttttgcctg cgtctgaccg ccgggcgcgg gactgcgcac
      901 cagccgggac gaatgcttgc actgaaagag ggcggacgca ccacaggcgt cgcctatcga
      961 ctgccagaag agacgctgga gcaggaacta accctgttgt ggaagcgcga gatgattacc
     1021 ggctgttatc tgccaacctg gtgtcagctt gatcttgatg atggacgcac agtaaacgcc
     1081 attgtgttta ttatggaccc gcgacatcca gaatatgaat ctgacactcg cgctcaggtc
     1141 atcgcgccgt tgattgcggc ggcgagcggt ccactgggaa ccaacgcaca atacctgttt
     1201 tcactggaac aggagcttat caaactggga atgcaggacg atgggctgaa tgatttgctg
     1261 gtatcggtaa aaaaactgct ggcggagaat tatccggatg gtgtgttacg gccgggattc
     1321 gcctgagtaa acttccctca tagtggggcg tcagacgccc cctcaaacac taaaatgtga
     1381 gcactttatc ggctgacagc gtccattgcg ccagttccac aagcgtaccg atttccaccc
     1441 catcaatcag aggaagtgtg ctaatcccgc gtccgtcggt acaggttttg cacaatttca
     1501 ccggtacatt ctgagcggta aggatctcca gcatttgctg aatgttgtag ccttcccctg
     1561 gtttttgccc gcgcaacccg gctgtgaccg catcagacat caggaacagg cgcagatcca
     1621 gattgctctc ctgctctcgt aacgcaatgg ccagccgcaa gctgttaaac aaggattcgc
     1681 tcccgtaagg tgcgccattg gcaacgatca cgattttttg cattatttac tcctgtattc
     1741 agggaattag acactcatct tctatcttac tgcttctgca gcgtctgacc aatcggtcac
     1801 atttttaagg attttcctga aagcgcgaga aaatacgaca aaagttgcca gtaatcgtta
     1861 ttctttaggg ctatggtttt tcatttttta ccggaagtta ccgacgtttt gagccgtttc
     1921 gttcctcgca ttattccgtt ttatttactc ttgctggcgg caggcggtac agctaacgca
     1981 caatctacct tcgagcaaaa agcggcaaat ccctttgata ataacaatga tggtctgccg
     2041 gatttaggca tggcacctga aaatcatgat ggggaaaaac actttgctga aattgtgaaa
     2101 gatttcggcg aaaccagtat gaatgataac gggctggata ctggcgagca ggcaaaagct
     2161 ttcgcattgg gaaaagtccg cgatgcgctt agtcaacagg ttaatcagca cgtagagtcc
     2221 tggctatcac cgtggggaaa tgccagtgtt gacgtcaaag tggataacga aggacatttc
     2281 accggcagtc gtggaagctg gtttgtgccg ttacaagata atgatcgtta tctcacctgg
     2341 agccagcttg gtcttactca gcaggatgat gggctggtga gcaatgtggg cgttgggcaa
     2401 cgctgggcgc gcggcagctg gctggtgggt tataacactt tttatgacaa cttgctggac
     2461 gaaaatcttc agcgagcggg cttcggtgcc gaagcgtggg gcgaatattt gcgactatcg
     2521 gcaaactttt atcagccgtt tgctgcatgg catgaacaga cagccacgca ggaacaacgg
     2581 atggcgcgcg ggtacgacct gacagcccgg atgcgcatgc cgttttatca acacctcaat
     2641 accagtgtca gcgtagaaca gtattttggt gatcgtgttg atttgtttaa ctctggtacg
     2701 ggttatcaca atcccgtcgc gttgagtctg ggattaaatt ataccccagt gccattagtc
     2761 actgtgacgg cccagcataa acagggtgaa agtggcgaga atcaaaataa cctcgggctg
     2821 aatcttaatt accgctttgg tgtaccgctc aaaaaacaac tttctgcggg cgaggttgcc
     2881 gaaagtcagt cgttacgtgg tagtcgctat gacaatccgc agcgaaataa tcttccgact
     2941 cttgagtacc gacagcgaaa aacgttaacg gtgtttctgg cgacaccgcc gtgggatcta
     3001 aaacctggcg aaacagtgcc gctgaaatta caaatccgca gtcgttatgg tattcggcaa
     3061 ctgatttggc agggcgatac gcagatatta agtttaacgc caggcgcaca agccaacagc
     3121 gcggagggct ggacgctgat catgcctgac tggcaaaacg gggaaggggc aagcaatcac
     3181 tggcgattgt cggtggtggt ggaagataac caggggcagc gtgtctcctc caatgagatc
     3241 acgctaacgc ttgtcgaacc gttcgacgca ttgtcaaacg acgaactgcg ctgggaaccg
     3301 taatcagaaa atgcgctcct gatgcaccca taccgctgct tccacgcgag acttgagctt
     3361 cattttcttc agcatgtgct tgacgtgcac ttttactgtg ctttcggtga tgtccaggcg
     3421 gcgggcaatc atcttgttcg gcaaaccctg ggcaatcagc ttgagaatat cgcgctcgcg
     3481 tggggttaac tggttaacat cgcgctcagt agtggcacgg ttagcgcgca agctggcggc
     3541 cagaacaggc gttaatgctt cgcttaatac catttcgcca gcagctgcct gatgcaatgc
     3601 tttcagcaga tcttccggtt ccatatcttt taacagatag ccatccgcgc cgcgtttcag
     3661 tgcggtgacc acatcttctt catggttaga gacgctgaat accacaatgc gccctgagag
     3721 ggacttttcg cggagtttat ccagcgtttc cagaccattc atgccgggca tattgagatc
     3781 taacaggatc agatcgggat caagagattc cgccagttca ataccctgtt cgccattact
     3841 cgcttcgcca accacgctga tatctggtgc catactgata agctgtttta cgccagttcg
     3901 cagcatcggg tgatcgtcga tcagcaggat agtagccggt tcctgattac tcatgggtat
     3961 ctccttggac gtctgtgaaa gttttttcgg gaataaaggt gacaaccact tcggtgccac
     4021 ctgattcacg acggcggacg cggcaatcgc ctcgtaaact ttgcgcacgg tcgcgcatta
     4081 ttatcatgcc gtagtgattg ctgcggatgg cattttcagg cacgccgcag ccgttatcct
     4141 ggacggtcag tttgacctga ttatcgtttt gcgccaccgt caccaagacc tcactcgctt
     4201 gcgaatgttt gagggcgtta cttaatgcct cacgggcaat ttgcaacaag tggattgcct
     4261 gatgcgaagg caccagacga ggtggcaatt gataatccag cttcaccggg aagccaaatt
     4321 tggcgctgta ctcttcgcaa ctcgcctcca gcgctggacg taatccaggc tcggtgagct
     4381 gcaagcggaa tgtggtgagc aattcacgca actgcgccca ggatgcattc agttcgttac
     4441 ggatctgact taacagttcg cggctgcttt ctggcagcgc atcgccctgc atctgtaaac
     4501 aactcacctg catcttcatg caagagagag attgggcaat agaatcatgc agttcgcgcg
     4561 caatggtggc acgctcttcc atcacgatca actgttgctg acgttcctga tggcgatcca
     4621 acgccagcgt ggcggtgagt tgttcaacca gggtatccac cagttgttgt tgatcatggc
     4681 taagatgacg cccctgcggt agggtcgcca gcaaaatgcc gtactgcgta tgagagtcag
     4741 ccagccgcca cttcagggtt gtgccgcgat cgccaacggg taatacgccg cgcgggcaga
     4801 gctggcagcc tttatcatca caagtcatat ctggctggca ggtaaactcc tgatgattct
     4861 cttcatcatc agtgtcatac acccgcagtt cgatatcacg tagcagggtc aaattctgta
     4921 agccgttgag tacaggtgac aggcgttcac acagcggggc gcgggaatgc aaacggcggt
     4981 tagcctgcca taaaaaagag aggatctgat ttttatgctc cagcccggca gttttctcct
     5041 gaacccgctg ctcaagtacg gcataacttt cggccagttc tgcagacata ttgttcaatg
     5101 cagttccaag catcgccatt tcgttgcgcc cgctgatgtt tgcgcgttgg gtaaaatcgc
     5161 gatggctgac ggcactcgcc attgccagca gttgccgcca cggttgtagc aatcgcgccc
     5221 gcaaccagat aatagtgaac accagtaaaa gtgccataaa taccgccatt acccgatgga
     5281 ccagcaccac tgtctcaatg cgcatttccg tggtgcggtc aaaaccagat accagctgat
     5341 caagcccggc aacaaactgg ctgacatccg ctgacaccgt ttcgcggttt tgtgcacgca
     5401 tcagcgtagg gatcagttca ttacgccagt aatcttgtaa accctgtaat tgcgccagtt
     5461 gtccgtctcc ttctgctgct cgggttaact cggcgctaaa tgccgtttgt tccatttctt
     5521 taattaaggg cttgtctttc tcgcttaatg gcactgctgc caacagacgg taactttgca
     5581 tgcgcagcga tcccgctttg ttgatcgcat gggcgctgcc ctgaacgcct tgcaccagcc
     5641 agccagaaac cgccatccct gccagtccaa tagcagtaga aagcaacaca ataagcgcaa
     5701 cctgattaac cagggtgagc ggagagagac aacgtttaag catgtaaacc tcttccttca
     5761 ggctttaaat gagcaataac cttaatgaat gtgacgatac attctggaat ggcagtattc
     5821 tcggctattg gctgaagtat acccataccc ggaaagagtt actccttatt tgccgtgtgg
     5881 ttagtcgctt tacatcggta agggtaggga ttttacagca ccgtgaaaaa tctcataatt
     5941 tttatgaaat cactgtactc actatgggta atgataaata tcaatgatag ataaagttat
     6001 cttatcgttt gatttacatc aaattgcctt tagctacaga cactaaggtg gcagacatcg
     6061 aaacgagtat cagaggtgtc tatgagtcac tcatccgccc ccgaaagggc tactggagct
     6121 gtcattacag attggcgacc ggaagatcct gcgttctggc aacaacgcgg tcaacgtatt
     6181 gccagccgca acctgtggat ttccgttccc tgtctgctgc tggcgttttg cgtatggatg
     6241 ttgttcagcg ctgttgcggt gaacttacca aaagttggtt ttaattttac gaccgatcag
     6301 ctatttatgt tgactgcgct gccttcggtt tctggcgcgt tattacgtgt tccatactcc
     6361 tttatggttc ctatcttcgg tggtcgtcgc tggacggcgt tcagcaccgg tattctgatt
     6421 attccttgcg tctggctggg ttttgccgtg caggatacct ccacgcctta tagcgtcttc
     6481 atcatcatct ctctgctgtg cggctttgct ggcgcgaact tcgcttccag tatggcaaac
     6541 atcagcttct tctttccgaa acagaagcag ggtggcgcgc tgggtctgaa tggtggtctg
     6601 ggcaacatgg gcgtcagcgt catgcagttg gttgctccgc tggtggtatc actgtcgatt
     6661 ttcgcagtat ttggtagcca gggtgtcaaa cagccggatg ggactgagct gtatctggcg
     6721 aatgcgtcct gggtatgggt gccgttcctt gccatcttca ccattgcggc gtggtttggc
     6781 atgaacgatc ttgctacctc gaaagcctcc atcaaggagc agttgccggt actcaaacgg
     6841 ggtcatctgt ggattatgag cctgctgtat ctggcaacct tcggctcctt catcggcttc
     6901 tccgcgggct ttgcgatgct gtcaaaaacg cagttcccgg atgttcagat tctgcaatac
     6961 gctttcttcg ggccgtttat tggtgcgctg gcgcgttctg caggtggtgc attatctgac
     7021 cgtctgggcg gaactcgtgt cacactggtg aactttatcc tgatggcgat tttcagcggc
     7081 ctgctgttcc tgaccttacc gactgacggg cagggcggaa gcttcatggc gttcttcgcg
     7141 gtcttcctgg cgctgttcct gacagctggg ctgggtagtg gttccacttt ccagatgatt
     7201 tccgtgatct tccgtaaact gacaatggat cgcgtgaaag cagaaggggg ttctgacgaa
     7261 cgtgcgatgc gtgaagcggc aaccgacacg gcggcggcgc tgggtttcat ctctgcgatt
     7321 ggcgcgattg gtggcttctt tatcccgaaa gcgttcggta gctcgctggc attaacgggt
     7381 tcgccagtcg gcgcaatgaa agtatttttg attttctata tcgcctgtg
//
LOCUS       DARNVM010000203         7423 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.203, whole genome shotgun sequence.
ACCESSION   DARNVM010000203 DARNVM010000000
VERSION     DARNVM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.203"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            148..1515
                     /locus_tag="SI621_004252"
     CDS             148..1515
                     /locus_tag="SI621_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEI4066896.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVVG
                     GPIVAHILSSHYESYNLHIAELTNENDQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDSLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVSIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            complement(1620..1707)
                     /locus_tag="SI621_004253"
     tRNA            complement(1620..1707)
                     /locus_tag="SI621_004253"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Ser,seq:gga)
     gene            1941..2879
                     /gene="ghrA"
                     /locus_tag="SI621_004254"
     CDS             1941..2879
                     /gene="ghrA"
                     /locus_tag="SI621_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HEI4066897.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWLHPRVAITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2934..3671
                     /gene="ycdX"
                     /locus_tag="SI621_004255"
     CDS             2934..3671
                     /gene="ycdX"
                     /locus_tag="SI621_004255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HEI4066898.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3695..4249
                     /gene="ycdY"
                     /locus_tag="SI621_004256"
     CDS             3695..4249
                     /gene="ycdY"
                     /locus_tag="SI621_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HEI4066899.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4351..4842
                     /locus_tag="SI621_004257"
     CDS             4351..4842
                     /locus_tag="SI621_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEI4066900.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLFS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAVRSTKTAHREQEIK
                     NKA"
     gene            complement(4906..5739)
                     /gene="csgG"
                     /locus_tag="SI621_004258"
     CDS             complement(4906..5739)
                     /gene="csgG"
                     /locus_tag="SI621_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HEI4066901.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5766..6182)
                     /gene="csgF"
                     /locus_tag="SI621_004259"
     CDS             complement(5766..6182)
                     /gene="csgF"
                     /locus_tag="SI621_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HEI4066902.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6207..6596)
                     /gene="csgE"
                     /locus_tag="SI621_004260"
     CDS             complement(6207..6596)
                     /gene="csgE"
                     /locus_tag="SI621_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HEI4066903.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6601..7251)
                     /gene="csgD"
                     /locus_tag="SI621_004261"
     CDS             complement(6601..7251)
                     /gene="csgD"
                     /locus_tag="SI621_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HEI4066904.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1919 a         1607 c         1792 g         2105 t
ORIGIN      
        1 tataaagcac taaggtaata ataattgcag tctgcaacta tcatcatcgg tattgtttgt
       61 gggcggaatt tcatattgac aacagtacag ttcttatatc tattaataat agaaagggat
      121 ctacaaccta cagattggtg tagctttatg gaaaaagact atttgagaat tagtagtact
      181 gtattagtga gcttattgtt tgggcttgct ttggttttgg tgaatagttg gtttaatcag
      241 ccaggcgttg aggaagttgt tccacgatca acctatctga tggtaatgat tgctttattt
      301 tttatcgata ctgttgcatt tatttttatg cagttgtatt tcatttatga ccgtaggcaa
      361 ttttcaaact gtatacttag tttggctttt ctgagttgtt tgatttactt tgttaaaacc
      421 gtcattatca ttcagcaaat tattgagggg cgtttgacaa gcagtgttgt tcaaaatgac
      481 attgcaatct attatttgtt tcgtcagatg agtttgtgca tattaatatt tctggcattg
      541 gtgaataaag ttagtgaaaa cacaaaacag cgcaatttat tttccaaaaa aatgactttg
      601 tgcataagtt tgttttttgt tgttgggggg ccgatagttg ctcacatact ttctagtcat
      661 tatgagagct acaatttaca cattgccgag ttaaccaatg aaaatgatca ggtggtttgg
      721 aaaacatcat atgttaccat aatgattttc atgtggttaa ccttgctttc agttaatcta
      781 tactttaatg gtttacgctg tgatatctgg aatggagtga cggtgattgc tttttgtgcc
      841 gtactgtaca atgtctctct tttatttatg agcaggtata gcgtgtccat ttggtatatt
      901 agtcgcacta ttgaagtagt cagtaagtta accgttatgg taatatttat gtgccatata
      961 ttcagtgcgc tacgagtaac aaaggacatt gcacatcgcg attccttaac caatatattt
     1021 aacagaaatt atttttttaa tgaactgaca gttcaatcag catcggccaa aaaaacgcct
     1081 tattgcgtca tgattatgga tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat
     1141 ccggttggcg atcaggtgat aaaaacagtg gtgagtatca ttgggaaaag catacggcca
     1201 gatgaccttt ttgcgcgcgt cggcggcgaa gagtttggcg tcttgctgac ggacatcgat
     1261 actgaacgtg cgaaagcttt agcggaaagg attcgggaaa atgttgagcg tttaactggt
     1321 gataatcctg aatacgccat accacaaaaa gtgacgatta gtattggcgc tgttgtaacg
     1381 cagaaaaatg agttaaaccc taaggagatc tatcaactgg ctgataacgc actttacgag
     1441 gcgaaagaga ccgggcgtaa taaggtggta gtaaaagagg cagaaattct tatcaaccgc
     1501 aaggatgatg aatgagtctg tcgctagttg tcgacctccg cctggggctt ctcatccccc
     1561 cggtctatgc aatatacgaa aaaaaagctc gtactttcgt acgagctctt ctttaaatat
     1621 ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg
     1681 ctccttcagc cactcggaca cctcaccaaa ttgttgttcc tgtcttgctg gaacgggcgc
     1741 taatttaggg aaatcatgac ctgaggtcaa caaacttttt taaaaaatcg cgcgtttatt
     1801 caaacttcaa tcaatatgca gttttaataa gcgaaatctg ctttttttgc caccgaccac
     1861 ggatttgtta tgctggtggc ttttgtagat cataacgata agtgcgaata aatttcgcgc
     1921 aacgcttttc gggagtcagt atggatatca tcttttatca cccaacgttc gatacccaat
     1981 ggtggattga ggcactgcgc aaagctattc ctcaggcaag agtcagagca tggaaaagcg
     2041 gagataatga ctctgctgat tatgctttag tctggcatcc tccagttgaa atgctggcag
     2101 ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt tgattctatt ttgagcaagc
     2161 tacaggcaca ccctgaaatg ctgaagcctt ctgttccact ttttcgcctg gaagataccg
     2221 gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt gctgcattgg tttcgacgtt
     2281 ttgacgatta tcgcatccag caaaatagtt cgcattggca accgcttcct gaatatcatc
     2341 gggaagattt taccatcggc attttgggcg caggcgtact gggcagtaaa gttgcacaga
     2401 gtctgcaaac ctggcgcttc ccgctgcgtt gctggagtcg aacccgtaaa tcgtggcctg
     2461 gcgtgcaaag ttttgccgga cgggaagaac tttctgcatt tctgagccaa tgccgggtat
     2521 tgattaatct gttaccgaat acccctgaaa ccgtcggcat tattaatcaa caattactcg
     2581 aaaaattacc ggatggcgcg tatctcctca acctggcgcg tggtgttcat gtcgtggaag
     2641 atgacctgct cgcggcgctg gatagcggga aagttaaagg cgcaatgttg gatgttttta
     2701 atcgcgaacc cttaccgccg gaaagtccgc tctggctaca tcctcgcgtg gcgataacac
     2761 cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga gtacatttct cgcaccattg
     2821 cccagctcga aaaaggggag agggtctgcg ggcaagtcga ccgcgcacgc ggctactaat
     2881 aaagcatcag gtttcctgct atccttggcg ggaattgaat acaggagaga gttatgtatc
     2941 ccgtcgacct tcatatgcat accgttgcca gcacgcatgc atatagcaca ttgagtgatt
     3001 acattgccca ggccaaacaa aagggcatta aactttttgc gatcaccgat catggcccgg
     3061 atatggaaga tgcgccgcat cactggcact tcattaacat gcgtatctgg ccgcgagtgg
     3121 ttgatggggt tgggatcctg cgcggcatcg aagctaacat taaaaatgtt gacggtgaaa
     3181 tcgactgcag tggcaaaatg tttgattcgc tggatctgat tattgccggt tttcatgagc
     3241 cggtgtttgc gccgcatgac aaagcgacta atacgcaagc gatgatcgcc actatcgcca
     3301 gcggcaatgt gcatataata agccatcccg gaaatccgaa atatgaaata gatgttaaag
     3361 ccgttgctga agcagctgcg aaacatcagg tggcgctgga aatcaataat tcctcatttt
     3421 tacactcacg taagggcagt gaagacaact gtcgggcggt agccgcagcg gtacgtgacg
     3481 ctggaggttg ggtggcatta ggctcggatt ctcacaccgc gtttaccatg ggtgaatttg
     3541 aagagtgtct taaaatcctc gacgcggtag attttccgcc agagcgcatt ttgaatgttt
     3601 ctccgcggcg cttactgaac ttccttgaat ctcgcggtat ggcaccgatt gcggaatttg
     3661 cagaccttta attactagta atggaaatat attaatgaac gagttttcta tcctctgtcg
     3721 tgtgctgggt tcgctctatt accgccaacc gcaagatcct ttactggtgc cgctgtttac
     3781 cctgattcgt gaggggaaac tggctgcgaa ctggccactg gagcaggatg agttactgac
     3841 gcgtttacag aaaagctgtg atatggcgca agtctctgcc gattacaatg cgttgtttat
     3901 cggcgatgaa tgtgctgtgc cgccatatcg tagcgcatgg gttgagggcg cgacggaagc
     3961 ggaagtgcgc gcttttcttt ccgaacgggg gatgccatta gcggatacgc cagccgatca
     4021 catcggcaca ttgctgctcg cggcttcctg gctggaagat cagtcaacgg aagatgagag
     4081 cgaagcactg gaaacactgt tcagtgagta tctgttaccc tggtgtggtg cgttccttgg
     4141 caaagtggag gcccatgcaa ctacgccttt ctggcgcacc atggcaccgc taacccgcga
     4201 tgctattagt gcaatgtggg acgagctgga agaagattct gaagagtaaa tgtgatctgc
     4261 atcactcata actgcaacag ataatttgtt gttgcataaa atgtgtgctc gatctcattc
     4321 atggccgcgt tttctgctat catgcgcggc atgaacatac ttctctctat tgcaatcaca
     4381 acaggcattc tctccggtat ctggggatgg gtggctgttt ccctgggctt actaagctgg
     4441 gcgggcttcc tgggctgtac ggcctacttt gcctgcccgc aaggtgggct gaaagggctg
     4501 gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg cgatggtcat tatttacggt
     4561 agtgcactgg caccacatct ggaaattctc ggttatgtca taaccggtat tgtcgcgttt
     4621 ctgatgtgta ttcaggccaa acagctgctg ttttcatttg ttccgggaac gtttataggc
     4681 gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg tgttaccttc gctggcgctc
     4741 gggttgatat ttggttacgc aatgaaaaac agcggccttt ggctggcggt gcgtagtacg
     4801 aagaccgctc accgtgagca ggaaatcaaa aataaagcgt gaggggcact cacgctttcg
     4861 cttaaacagt aaaatgccgg atgataattc cggcattttt atctatcagg attccggtgg
     4921 aaccgacata tggcggtatt tcaccagaat gtcattctgc cgttctgctt tgttctgcaa
     4981 atcccaaaga ccgcggtcga taccatcatt aatcaggaaa atgacgcctg tttcgatagc
     5041 cgacatcagg cacagcataa cgggttcgtt cgaggtgtaa cctacttccc cttccagcaa
     5101 gcgctggtag tcaataaagc ggaagacccc cgcctgaact tcataggaaa gtatcgtctt
     5161 actggtgttc accgaggaga ggatctcacc ggtactcaca ttgacgacgc gcaggttcac
     5221 ggcaatctga tcgagctggt attgcgtgtc ggcaccgatc ccaaaatatc ttgccccaac
     5281 accgcccgat ttaacgttgc tttcataacc gataatcgaa ccttcaacca tgatatttgc
     5341 cgccgttaaa gactgcagcg ggattcggtt attaatggca accgtaccgt tttcttgtgc
     5401 cgcacgaata atcttgcgct cgttaagcag gttttgtaag ccctggcgct ccagcggtat
     5461 aaaccagcga gaatctttca atgccgtgac cagcattgcc gtggcgcttt gcggaacagc
     5521 agtggagaag ttacttgccg ggtagggttt aaattgcccg gtttcgtcct gaatgttgta
     5581 taccgaaaca aagatcttac ccgtcggcgc tggcagatgg gtcaaatctt tgtagctctg
     5641 agcacgaggc attaatgtcg gtctggcggc ttctttaggc ggggcggtta agcatccgct
     5701 cagtaacatg acggcaacca aaagaaataa gcgctgcatg attattttcc ttatgaagct
     5761 ggggcttaaa aatcggttga gttattttgt aaacccgaaa cctggatggt cgaggtttgt
     5821 ccggttttac gatctgtcac gttcaactgc aattgaccat cgcggttagc aatatcaaca
     5881 atataatcgt tggtcaccat gcggcccggt ttaccggtat taatattcga cagtagccca
     5941 cctaaaattt gtgactggat ggcctgagta aagttatcta acgctgaggg tgtttcaata
     6001 ccatagtcat cgttatagct cggatcttta tacgagtttt gtgcctgggc gctatttaat
     6061 aaaaaagcgc cattatttgg gttaccacca aagtttggat tacggaactg aaaagtcatg
     6121 gttccagccc aacttaatgg cgaaataagc atgagtagaa ctactgcatg tttgacacgc
     6181 attgcagcct ccgaacaatt ttttatttag aattcatcat gcgccaaatc gcccgtactt
     6241 aataacgcct gatttatctg gcgacgattt agtgcttctt cagtttgaat cagtgcaaag
     6301 acgacagttt tttcgaagtc tcttttcaac gggaataaaa aagtctggaa aataacgtcc
     6361 tgattgaccg ttatagtgat ccagcttccc catcgtgcac tgggcctttc attaatcgtt
     6421 aagttacccg tatagtcact ttcccattta tcactaaagg ctcggtaaaa atcatggcca
     6481 atagatgaaa cagtatggtc agttaacaat cccgggactt ctacctcaac ggcgtgaaga
     6541 ttccctgcgg cgaacagaaa ttctgccgcc acaatccagc gtaaataacg tttcatggct
     6601 ttatcgcctg aggttatcgt ttgcccagga aaccgcttgt gtccggtttt ttacggctat
     6661 cttcttgaaa agattataaa gatgcgtttt aaccgtattt tcgctgatga acaacgaacg
     6721 agcgatctcg ttattagacg cgccgatacg cagcttattc aggatctctt tttcccgatg
     6781 agtaaggagg gctgattccg tgctgttata acgatagtta cctgaatgcg taatcaggta
     6841 actggcaagc ttttgcgtaa agtagcattc gccgcgcagg acgccttgca acccattgac
     6901 aacacgttct tgatcctcca tggcataaaa aacgccgttg atatgaggcc agttttcaat
     6961 gtcgcggtac gggtaatctt caggcgtatt tagcaacaat attttgatat tgttgttttt
     7021 cctgctcaaa gtatcctgcc aataatggat aagcttttta tccgcttcca tcatatccag
     7081 aagaataata gagcctgaag atatatcgtc cagagaacgt tgaatattat gtaattttcc
     7141 tgtaattgcc agcgattgtt taaggtgctg caagagagct gtcgcctgca aagaaggttt
     7201 ggtgatcaac aataatgtat gaccatgaat actatggact tcattaaaca tgatgaaacc
     7261 ccgctttttt tattgatcgc acacctgaca gctgcctcta aaatagaagc accagaagta
     7321 ctgacagatg ttgcactgct gtgtgtagta ataaatcagc cctaaatggg taaaatataa
     7381 aactaatgga ttacatctga tttcaatcta gccattacaa atc
//
LOCUS       DARNVM010000204         7378 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.204, whole genome shotgun sequence.
ACCESSION   DARNVM010000204 DARNVM010000000
VERSION     DARNVM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.204"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..429
                     /locus_tag="SI621_004262"
     CDS             <1..429
                     /locus_tag="SI621_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEI4066905.1"
                     /translation="GYAICRSMDLKSWEYVSDHRGEIVYSTETGESKEIKTPGDYPEN
                     ATTIAPSTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(401..475)
                     /locus_tag="SI621_004263"
                     /pseudo
     CDS             complement(401..475)
                     /locus_tag="SI621_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            491..646
                     /locus_tag="SI621_004264"
     CDS             491..646
                     /locus_tag="SI621_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001786072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4066906.1"
                     /translation="MWEQAGRLLAARESRQRVALIFDIDLSTLYKKFASSATKNKLCH
                     PSANRDK"
     gene            706..1896
                     /locus_tag="SI621_004265"
     CDS             706..1896
                     /locus_tag="SI621_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HEI4066907.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGEDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            1909..2427
                     /locus_tag="SI621_004266"
     CDS             1909..2427
                     /locus_tag="SI621_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HEI4066908.1"
                     /translation="MAMPRKLKYMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            2484..2759
                     /locus_tag="SI621_004267"
     CDS             2484..2759
                     /locus_tag="SI621_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEI4066909.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            2792..2911
                     /locus_tag="SI621_004268"
     CDS             2792..2911
                     /locus_tag="SI621_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HEI4066910.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            2904..5351
                     /locus_tag="SI621_004269"
     CDS             2904..5351
                     /locus_tag="SI621_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEI4066911.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMTALRRQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVARTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     TLRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTNAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            5366..5845
                     /locus_tag="SI621_004270"
     CDS             5366..5845
                     /locus_tag="SI621_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4066912.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMADEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKASSMATRFTGMTGAG"
     gene            5845..7008
                     /locus_tag="SI621_004271"
     CDS             5845..7008
                     /locus_tag="SI621_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HEI4066913.1"
                     /translation="MLDALTFDAGSTLMPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIATRNRLEASVASSLAEIKIPHIDQSQ
                     ESDVKFLTRLAERNGGEVSVKMGKLLFLKAGQGATASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            7090..7308
                     /gene="ogrK"
                     /locus_tag="SI621_004272"
     CDS             7090..7308
                     /gene="ogrK"
                     /locus_tag="SI621_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HEI4066914.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
BASE COUNT         1860 a         1869 c         2140 g         1509 t
ORIGIN      
        1 ggttatgcta tctgccgttc tatggattta aaatcatggg aatatgtgtc agaccatcgt
       61 ggtgaaattg tctatagcac cgaaacaggt gaatcaaaag aaatcaaaac tccgggtgat
      121 taccctgaaa atgcaaccac tatcgccccg tcaacgccat acgataaatg ggatggtgag
      181 aaatgggtga cggataccga ggcacagcat agcgccgcag tagatgcagc agaagcacag
      241 cgccagtcac tgattgatgc agcaatggct tccattagtc tgattcagct gaaattacag
      301 gccgggcgga aactgacgca ggcagaaaca acccggctta acgctgtgct ggattacatt
      361 gacgcggtga cggcaacaga taccagcacc gcgcctgatg ttatctggcc tgaactgccg
      421 gaggcgtagg ccattcaata tctggagcac tggaaatttc gacaagttcc agtgctccag
      481 ataatcgact gtgtgggaac aggcaggacg attgctggct gcgagggaat cacgtcaacg
      541 tgtggcgctg atttttgata ttgacctgtc cacgctctat aaaaaattcg cctcatcagc
      601 gacaaagaat aaattgtgcc acccatcagc caaccgggac aaatagcctg acatctccgg
      661 cacacatgaa aataccactc acccattaac cacggagtta aacggatgag tgactatcat
      721 cacggcgtgc aggtgttgga gattaacgac ggcacccgcg tcatttccac cgtatccact
      781 gccattgtcg gcatggtctg cacggccagc gatgcggatg cggaaacctt ccccctcaat
      841 aaacctgtgc tgattaccaa tgtgcagagc gcaattgcaa aggccggtaa aaaaggcacg
      901 ctggcggcat cgttgcaggc catcgctgac cagtcaaaac cggtcaccgt tgtcgtgcgt
      961 gtggaagacg gcaccggcga agacgaggaa acgaaacttg cgcagaccgt ttccaatatc
     1021 atcggcacca ccgacgaaaa cggccagtac accggactga aagccctgct ggcggcggag
     1081 tcggtaaccg gtgttaaacc gcgtattctt ggtgtgccgg ggctggatac caaagaggtg
     1141 gctgttgcac tggcatcagt ctgtcagaag ctgcgcgctt tcggatatat cagcgcatgg
     1201 ggctgtaaga ccatttccga ggtgaaagcc taccgccaga atttcagcca gcgtgaactg
     1261 atggttatct ggccggattt cctcgcatgg gatacggtca ccagtaccac cgccaccgcg
     1321 tatgccaccg cccgtgcgct gggtctgcgc gctaaaatcg accaggagca gggctggcat
     1381 aaaacgctgt ccaacgtcgg ggtaaacggt gttaccggca tcagcgcatc tgtattctgg
     1441 gatttgcagg agtccggcac cgatgctgac ctgcttaacg agtcaggcgt cactacgctg
     1501 attcgccgcg acggtttccg attctggggt aaccgtacct gctctgatga cccgctgttc
     1561 ctctttgaaa actacacccg caccgcgcag gtgctggccg acacgatggc tgaggcgcac
     1621 atgtgggcgg tggacaagcc catcaccgca acgctgattc gcgacatcgt tgacggcatc
     1681 aatgccaaat tccgtgagct gaaaacaaac ggctatatcg tggatgcgac ctgctggttc
     1741 agcgaagaat ccaacgatgc ggaaaccctc aaggccggaa aactgtatat cgactacgac
     1801 tataccccgg tgcctcctct cgaaaacctg accctgcgcc agcgtattac tgataaatac
     1861 ctggcaaatc tggtcacctc ggttaacagc aattaaggag cctgaccgat ggcaatgccg
     1921 cgcaaactca aatacatgaa tgtctttctg aacggctaca gctatcaggg cgttgcaaag
     1981 tccgtcacgc tgccaaaact gacccgtaag ctcgaaaact atcgcggtgc ggggatgaac
     2041 ggcagcgcac cggtagacct cggccttgat gacgatgcgc tgtcaatgga gtggtcgctt
     2101 gggggcttcc cggattcggt tatctgggag ctttacgctg caaccagtgc tgatgccgta
     2161 ccgattcgtt ttgctggttc ttaccagcgc gacgataccg gcgaaacggt ggccgtcgaa
     2221 gtggtcatgc gtgggcgtca gaaagaagtc gacacaggag agggtaaaca gggagaggat
     2281 accgaatcga aactcccggt gatttgtact tatttcaagc tgacgatgga cggtaaggag
     2341 ctggtcgaaa tcgacaccat caacatgatt gagaaggtga acggcgtcga ccggctggag
     2401 caacaccgcc gcaatatcgg cctgtgattt tcatccggtc agcctggctg accggttaac
     2461 cccgattcag aagtgagaaa accatgaaca aagaaaacgt cattaccctg gacaatccgg
     2521 tcaaacgtgg tgagcaggtt atcgaacagg tcacgctgat gaaacccaat gccgggacgc
     2581 tgcgcggtgt cagtctggct gcagtcgcga actccgaagt cgatgcactg attaaagtgc
     2641 tgccgcgcat gacggcaccg atgctgaccg agcaggaagt cgccgcgctg gaactgcctg
     2701 accttgtggc gctggccggt aaggtggtcg gttttttgtc gccgaactcg gtgcagtgac
     2761 gtttccgaaa aatctctcgg tcgatgacct gatggcggat gtggcagtga tatttcactg
     2821 gccgccatca gaactgtatc ccatgagcct gaccgaactc atcacatggc gcgaaaaggc
     2881 gctccggcga agcggaaaca cgaatgagta acaatgtaaa attacaggta ttgctcaggg
     2941 ctgttgacca ggcatcccgc ccgtttaaat ccatccgtac agcgagcaaa tcgctgtcgg
     3001 gggatatccg ggaaacacaa aaatcactgc gcgagctgaa cggtcaggca tcccgtattg
     3061 agggatttcg caagaccagt gcacagctcg ccgtgactgg tcatgcactt gaaaaggctc
     3121 ggcaggaagc agaagccctt gccacacagt ttaaaaacac cgaacgtccg acccgtgctc
     3181 aggcgaaagt gctggaatcc gcaaagcgtg cggcggagga cttacaggcg aaatataacc
     3241 gcctgacgga ttctgttaaa cgccagcagc gggaactggc cgctgtggga attaataccc
     3301 gcaatcttgc acatgatgag caggggctga aaaaccgtat cagtgaaacc accgcacagc
     3361 ttaaccgtca gcgtgatgcg ctggcgcgtg tcagtgcgca acaggcaaaa cttaacgcag
     3421 taaaacagcg ttatcaggcc ggaaaggaac tggccggaaa tatggcctcg gtgggcgctg
     3481 ccggtgtggg gattgcggcg gcgggaacga tggccggagt taagctgctg atgcccggtt
     3541 atgagtttgc gcagaaaaac tcagaattgc aggccgtgct gggtgtggca aaagactccg
     3601 ccgaaatgac cgcactccgc aggcaggcgc gccagctcgg cgacaatacc gcagcctcgg
     3661 cagatgatgc agccggtgca cagattatca ttgcgaaagc gggtggagat gctgcggcta
     3721 ttcaggcagc aacgccggtc acactgaata tggcactggc aaaccagcgg tcgatggaag
     3781 aaaacgcgca attgttgctg gggactaagg catcctttca actgtcaaat gatgatgtca
     3841 gccatgtggg cgacgtgttg tcggcaacga tgaataagtc ggcggctgat tttcagggtc
     3901 tcagtgatgc actgacttac ctcggcccgg ttgcgaggac ggcaggtgta agccttgagc
     3961 aggcagcggc catgaccggt gtgctgcatg acaataacat cagggggtca atggcgggta
     4021 cgggtagcag tgccgttgtc acccgattac aggcaccgac tggaaaagca tgggatgcac
     4081 tcaaagagct tggcgttaaa acctcggaca aaaagggaaa tatgcgtccg ttgttcacca
     4141 ttctgaaaga gattcaggcc agctttgata aacacaagct gggaacgtct cagaaggggg
     4201 aataccttaa aaccattttt ggtgaggaag ccctgaaatc agcgaacgtt ttactggcag
     4261 cggcagcaag cggaaaactg gataagctga ccgccacgct gaaagcctcg gacggtaaaa
     4321 cggaagagct ggttaaaatc atgcaggata acctcggtgg tgactttaag gagtttcagt
     4381 ccgcttatga ggcggtgggg actgacctgt ttgaccagca ggaaggcaca ctgcgtaagc
     4441 tcacgcagac ggccacaaag tatgtgttaa aactcgacgg ctggatccag aaaaacaaat
     4501 cactggcgtc aaccatcggc atcattgccg gtggcgcgct ggcgctgact ggcatcatcg
     4561 gtgcaattgg tcttgtagcc tggccggtta tcaccggcat caatgccatc atcgcggcag
     4621 caggcgcaat gggggcaatc ttcacgacgg ttggcagtgc tgttatgacc gccatcgggg
     4681 ctattagctg gccggttgtg gccgtggtgg ctgccattgt cgccggggcg ttgcttatcc
     4741 gtaaatactg ggagcctgtc agcgcattct ttggcggtgt ggttgaaggg ctgaaagcgg
     4801 catttgcgcc ggtgggggaa ctgttcacgc cactgaagcc ggtgtttgac tggctgggcg
     4861 aaaagttaca ggccgcgtgg cagtggttta aaaacctgat tgccccggtt aaagccacac
     4921 aggacaccct gaaccgttgc cgtgacacgg gcgtcatgtt cgggcaggca ctggctgacg
     4981 cgttgatgct gccgcttaat gcgttcaaca aactgcgcag tggtattgac tgggtactgg
     5041 aaaaactcgg tgttatcaac aaagagtcag acacacttga ccagaccgcc gcaaggacta
     5101 atgccgccac gtatggcacc ggtggttata ttccggcgac cagctcttat gcaggctatc
     5161 aggcttatca gccggtcacg gcaccggctg gccgctctta tgtagaccag agtaaaaacg
     5221 aatatcacat cagcctgacg ggtggtactg cgccggggac acagcttgac cgccagttac
     5281 aggatgcgct cgaaaaatac gagcgggata aacgtgcgcg cgcccgtgcc agcatgatgc
     5341 atgacggtta aggaggtgac gaaaaatgat gctcgcgtta ggtatgtttg tttttatgcg
     5401 ccagacgctg ccacaccaga ccatgcagcg tgaatcagat tatcgctggc cgtcaaattc
     5461 ccgtatcggt aaacgggatg cctaccagtt tctcggtgtt ggcgaggaaa acatgacgct
     5521 tgccggtgtg ctttatcccg aactgaccgg cggaaagctg acgatgacca cgctcaggct
     5581 gatggcagat gaaggccggg cgtggccgtt gctggatggc accggcatga tttacggcat
     5641 gtatgtcatc agcaaggtga gtgaaacagg gagtattttc tttgcagacg gcacaccccg
     5701 gaaaattgat tttacgctgt cgctcacccg cgttgatgaa tcactggccg cgctttatgg
     5761 cgatatcggt aaacaggcgg aatcgctcat tggtaaggct agcagtatgg cgaccagatt
     5821 cacgggtatg acgggggcgg gataatgctg gatgcactga catttgatgc aggcagtacg
     5881 ctgatgccag attacatgct gatgctcgac agcagggata ttaccggcaa tatcagcgac
     5941 cgtctgatga gcatgaccct gacggataac cggggctttg aggctgacca gcttgatatt
     6001 gaactgaacg atgccgacgg gcaggtcgga ctgccggttc gtggcgctgt tctgacggtg
     6061 tatatcggct ggaaaggttt tgccctggta tgcaaaggga aatttaccgt tgatgaggtt
     6121 gaacaccggg gcgcgccgga tgtggtcacc atccgcgccc ggagtgcaga ttttcgcggg
     6181 acgctcaatt cccgccggga aggctcctgg catgacacca cgcttggtgc gattgttgag
     6241 gcgatagcca cccgtaacag gctggaagcc agtgtcgctt cgtcgctggc cgaaattaaa
     6301 atcccgcaca tcgaccagtc gcaggagtcc gatgtgaaat tcctgacccg tcttgctgaa
     6361 cgcaacggcg gtgaggtgtc ggtaaaaatg ggaaaactgt tgtttctcaa agcggggcag
     6421 ggggcgacgg ccagcggtaa aaaaatcccg caggtcacca tcacccgcag cgacggcgac
     6481 cgtcatcatt ttgcgattgc tgatcgtgga gcctacaccg gcgtaacggc aaagtggtta
     6541 cacaccaaag acccgaagcc gcaaaagcag aaggtaaaac tgaaacgcaa aaagaaagag
     6601 aaacacctgc gcgcactgga gcacccgaaa gcgaaaccag tcacgcagaa gaaagcgcca
     6661 aaagtaccgg aagcgcgcga aggtgaatac atggccggtg aggctgacaa cgtttttgcc
     6721 ctgaccacgg tatatgccac gaaagcgcag gccatgcgcg ccgctcaggc gaagtgggac
     6781 aaactgcaac ggggcgttgc ggagttctcc atcagccttg ccactggtcg ggctgatatt
     6841 tacacggaaa cgccggtcag agtgtcaggc tttaagcgcg tcatagacga gcaggactgg
     6901 acaatcacta aggtgacaca ttttctgaat aatagcggct tcacgacgtc cttagagctt
     6961 gaggtcaggc tttctgatgt ggagtacgaa acagaagatg atgagtgatg tttttatttt
     7021 atctgtttgt tttataagga taaattaact aaaatggcac catcaacaaa accggaagag
     7081 gtgctcgcga tgtttcattg tcctttatgc cagcatgccg cacatgcgcg tacaagtcgc
     7141 tatatcactg acacgacaaa agagcgttat caccagtgtc agaacgtgaa ttgcagcgcc
     7201 acgttcatca cttatgagtc ggtacagcga tacatcgtga agccgggaga agtccacgcc
     7261 gtgaggccgc acccgttgcc gtcagggcag caaattatgt ggatgtaatt acaaacagaa
     7321 agcccctcag tcgaggggct ttctgttttg gggaatattc ttcattaaaa gtagtgga
//
LOCUS       DARNVM010000205         7350 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.205, whole genome shotgun sequence.
ACCESSION   DARNVM010000205 DARNVM010000000
VERSION     DARNVM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.205"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..856
                     /locus_tag="SI621_004273"
     CDS             <1..856
                     /locus_tag="SI621_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEI4066915.1"
                     /translation="DAELNSIRERLNIMKASPHNNNVWGATYNTRNNVTTDAGAGFEQ
                     TLIGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVGSYSLGGYASWEHES
                     GFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGLGGHIETGMRFTDGNWNLTPYAS
                     LTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKE
                     FVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSSLSGHLGVGYSHGAGVESPWNA
                     VAGANWSF"
     gene            complement(1221..1487)
                     /gene="minE"
                     /locus_tag="SI621_004274"
     CDS             complement(1221..1487)
                     /gene="minE"
                     /locus_tag="SI621_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEI4066916.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1491..2303)
                     /gene="minD"
                     /locus_tag="SI621_004275"
     CDS             complement(1491..2303)
                     /gene="minD"
                     /locus_tag="SI621_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEI4066917.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2327..3022)
                     /gene="minC"
                     /locus_tag="SI621_004276"
     CDS             complement(2327..3022)
                     /gene="minC"
                     /locus_tag="SI621_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEI4066918.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3542..3910
                     /locus_tag="SI621_004277"
     CDS             3542..3910
                     /locus_tag="SI621_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066919.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4013..4414)
                     /locus_tag="SI621_004278"
     CDS             complement(4013..4414)
                     /locus_tag="SI621_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEI4066920.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4655..4948
                     /locus_tag="SI621_004279"
     CDS             4655..4948
                     /locus_tag="SI621_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HEI4066921.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5020..5679
                     /locus_tag="SI621_004280"
     CDS             5020..5679
                     /locus_tag="SI621_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEI4066922.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5771..6217
                     /locus_tag="SI621_004281"
     CDS             5771..6217
                     /locus_tag="SI621_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEI4066923.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6424..7335)
                     /gene="hlyE"
                     /locus_tag="SI621_004282"
     CDS             complement(6424..7335)
                     /gene="hlyE"
                     /locus_tag="SI621_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HEI4066924.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1882 a         1641 c         1787 g         2040 t
ORIGIN      
        1 tgatgctgag ctaaacagta ttcgcgagcg gttgaacata atgaaagcga gtccacacaa
       61 caataatgtc tggggggcga cgtataacac ccgtaataat gtcaccaccg atgcgggtgc
      121 cgggtttgag cagacgctga tcggaatgac agtggggatc gacagccgta atgatattcc
      181 agaaggaatt gccacgctgg gcgcttttat gggttattcc cattcacata tcggttttga
      241 tcgcggaggt catggcagtg tgggcagtta ttctctgggc ggctatgcca gttgggaaca
      301 tgaaagtggt ttctatctgg acggtatcgt gaagctgaac cgctttgaaa gtaacgtagc
      361 cggtaaaatg agcagcggtg gagccgccaa cggcagttat cgcagcaacg ggctgggcgg
      421 tcacattgaa accgggatgc gatttaccga tggtaactgg aacctgacgc cgtatgcatc
      481 gttaacgggg ttcaccgctg ataaccccga atatcattta tccaatggta tggaatcgaa
      541 atcagtcgat acccgcagta tatatcgtga actgggcgca acgctgagtt acaacatgcg
      601 tctggggaac ggcatggaag ttgagccgtg gctgaaggcg gctgtgcgca aagaatttgt
      661 cgatgataac cgggtgaaag tgaataatga cggtaatttt gtcaatgatt tgtcgggcag
      721 acgtggaata tatcaggcag gtattaaagc ctcattcagc agttcgttaa gcgggcatct
      781 cggggtgggg tatagccatg gtgccggtgt ggaatccccg tggaacgcgg tggctggtgc
      841 gaactggtcg ttctgaccat caacgattaa actgcgcttc ggcgcagttt tcgtttacag
      901 ggtgttaaaa ggaaaaattc tgcggcaaaa aaagcccgcc agttacggcg ggaaacctca
      961 tcctatggga gaacaatgaa taatgaaatt gcggggttat catcacccag tatacccata
     1021 ctaacaataa ggttatttac tcagccaggt ataaacaatt tgatctgtgt ggggggaacg
     1081 gcattaaggt gtaaaggggg gaagcaatga gtagcatcag caagaatact cgccgctctt
     1141 atcaaacgtg gatgaggggg atgaaaaacg caaggcagag atgactctgc cttgaagaga
     1201 aatgcgcttt tacagcgggc ctatttcagc tcttctgctt ccggcaaggt cacgttcagc
     1261 tcaagaatag aaatatcgcc atctttttgc tcaagctgta cggttaccat ctcaggatca
     1321 atttgtacgt atttacaaat gacctcaaga atatctttac gcaactgcgg cagataatgc
     1381 ggttctgcat cgctgcgacg gcgttcagca acaataatct gcagccgttc ttttgcaatg
     1441 ttggccgtgt ttttcttccg cgagagaaag aaatcgagta atgccataac ttatcctccg
     1501 aacaagcgtt tgaggaagcc tttcttctct tcttcaatga agcggaaagg acgttcttct
     1561 cccaacagac gttctacggt atctgcgtag gctttacccg catcggcgtt aatgtcgaga
     1621 atgaccggtt caccctggtt agaggcgcgc aatactgatt gatcctctgg gatcacgccg
     1681 acgagtttga tgcgcaggat ctccagcaca tcttccatgc tcagcatgtc acctctgctt
     1741 acgcggcctg ggttatagcg cgttaacagc aggtgctctt taataggctc ttcgccattt
     1801 tctgcgcggc gtgatttcga cgccagaatg cctaaaatac ggtcagagtc gcgtactgag
     1861 gagacttccg ggttggtggt aataatggct tcgtctgcaa aatagagtgc cattagcgca
     1921 ccggtctcaa tccctgccgg ggagtcgcaa acaataaatt caaagtccat cgctttcaga
     1981 tcatcaagaa ctttggcgac cccttcacgg gtgagggcat ctttatcgcg agtttgcgat
     2041 gccggaagaa tatagagatt ttcagtacgc ttatctttaa ttaacgcctg atttagcgtt
     2101 gcatcgccct gaatgacgtt gacgaaatcg taaacgaccc gacgttcaca acccataatc
     2161 aggtcgagat tacgcaggcc gatatcaaaa tctatcacga cagttttctt tcccttctgg
     2221 gccaaaccag tggcgatggc cgcgctggag gttgtcttac caacaccccc tttgcccgaa
     2281 gtaacaacaa taatgcgtgc catagaaatt ccttgttaaa aagggatcaa tttaacggtt
     2341 gaacggtcaa agcgttttcg actaactgca gtcgcgccgc tttgccataa aattctgctg
     2401 ggatttgatc actcagccag tattcacctg cgatggacac caattccgcc atcaggttcg
     2461 tacaaaatat ttgcgtttcc cggtcaccac ttgcccctgc cagcgcacga ccgcgcatca
     2521 tgccatagac atgaatgttc ccatcggcaa tcaattcggc cccagcgcta acgtggcttg
     2581 taacaatcag atcacattgt ggagcataaa tacgctgacc ggaacgcacc ggggtatcta
     2641 ttaaacgcgt ttttgtgacc ggcgttgtat tttgcgctgg agcctgcggt gcgggagctg
     2701 gacgcggcgc tttttccttt ccttccgtca ggataggcag ccccattttt tcaatttcgg
     2761 ctttaagttg cgcatctttg cagccactta cgccaataac ccgcaaaccg gttgccgaaa
     2821 ccgccttatg catcgctgac cagtttaccg ggtcttccag tgcactgacg ttgagtacaa
     2881 cgggggcatg ttttaaaaat gcgggggcct gagcgatttt gtcttccagc gcctgatgga
     2941 taaccttagg ttctgcctca tgcagatgaa ccacagataa agtgaagcta ctgcctttaa
     3001 gctcgattgg cgtgtttgac atcctggcct tactcaatta gctattaatc atcgccagcg
     3061 cgcgatgatg ttccgaagac tataaggcat gttatagtct ggattatatt gaggcaagtc
     3121 accctcccat ttattcagag taaaagtcta ttctgtgata aatggcgctg attcatagct
     3181 taaaaaatac ccttgtcaat caacccattg ccgtcgtact tttgattctt cttatttacg
     3241 cttccttttt ccgcacccta atcgtgacag catctccaaa cctgtttaaa tatccagtat
     3301 tgaggcaagt gtattttttc accacgccag gctttcttaa ctttccattt tttgcggcat
     3361 cattaaagaa cagatacccg ttatcttaat atccgccaca gaattgctct atcatagaaa
     3421 ccgcagaaga cgtctgatac ttctgtcagg tcatcgtaac tcgtccaggt cagcggtttt
     3481 gattacggat tgatgacgaa atgagcataa gtgttgcacg gttggattat caggatgtgc
     3541 tatgaatatg atgcgtatta tttatatcgg attgtcaggt gtggggatga tgttctcatc
     3601 aatggcgtct ggacatgacg ctggcggact tcaatctcct gcgtgtggcg ttgtttgcga
     3661 cccgtatatt tgtgttaact cagatggcat ttccccagag ttaacaagga aatatctcgg
     3721 cgaaaaagcc gctgaaaact tacaatcagt acaaggctac gatcccagcg aatttacatt
     3781 cgctaacggt gtattttgcg atgttaaaga aaaattatgt cgtgatgatc gttattttgg
     3841 tgtggatggt aagcggagtg gaaaaatcaa tcaaaccacc acaaagatgt tatttatgtg
     3901 tcgtgagtga tgtcttgttt acattatgtt ctattcgtca ttaacctctt gagcgacaga
     3961 gaggagtggg gcgttaacgt ttatgacggg gagagtcccc gtcggttgac atttacttaa
     4021 tttgaatatc gacgttatat tttttagttt tgtttttacg ggcgtcgttt cgagtttgca
     4081 gcactcgcag ctcatatttg ccagaagcag ggagtgagta ttgtccgtga ctatcgagtt
     4141 ctggggaata tctggataaa tcaacagaat catcgattcc tggcccgaag aggtaagtat
     4201 cagcgccttc attagagata ctcacatgca ctttctgacc ttttttggca taaaaggtat
     4261 atgtatcgta atcgtatccc tttatttcgc cggagtactg agcgctgcta tggccttttc
     4321 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
     4381 ataatacagc cttactgatg ctcttgattt tcataggtag tctcctgttc agaatgagta
     4441 cgatgaactg ttataatata acaattcatc acaggagaat tccagtggct ttcctgtgtt
     4501 cggttaaatc ccggcgcggc atttttgccg ggggatcact tattaagggc gcatggactg
     4561 aacaaaccgc agaaatctac agctggctgg cttgtggcgc gggtttcatt gatgttagct
     4621 tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt atcgaagcag
     4681 caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc gtgtacctga
     4741 ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgc tggatgggcg
     4801 gaagaaactg gtcaatgccg atattgaaaa ggttaaacag gcattaaccg agcaaggtta
     4861 ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt ccgtcatggg
     4921 gcagaaaaca gacgacacga acaaataacc gatatccggc ggtggcatta tctttgtcgg
     4981 cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca tcacaactgg
     5041 caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag taactatgcc
     5101 aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt tattaaacca
     5161 gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt cggttcagtt
     5221 catcatgaag tcgaactggc ggtattgatt ggcgcgacgc tgcgtcaggc tacggaagag
     5281 catgtccgta aagccattgc cggttacggc gtggcgctcg atttgacatt gcgtgatgtt
     5341 cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ccaaagcgtt tgataactct
     5401 tgtccgcttt ccgggtttat tcccgcggcg gaattcaccg gcgatccgca aaatacaacg
     5461 ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga catgatccat
     5521 aaaatcgttc cgctgatcgc ttatatgagc aagttcttta ccctcaaggc cggtgacgtt
     5581 gtgctgacag gcacgcctga tggcgtcggc ccgttgcaaa gtggtgatga gctgacagtc
     5641 actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc gcctgaaagc
     5701 ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta acgtaatggc
     5761 ggaacacctg atgagcgatg tacctttctg gcaaagtaaa accctggacg aaatgagcga
     5821 tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata aactgatgga
     5881 tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca atattaaaac
     5941 ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cctgactgca ttaaattaac
     6001 ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc tgcgcttatc gcttgctggc
     6061 ggaaggtaaa gatttacctg cgtggcatcc gctgcttact ggttcgaaag cggcaatgca
     6121 tggcgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga ttgactggca
     6181 ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga tgttgatggg
     6241 ggctttctta atatacgtct ggatactcgc catttcgtag ccctgttttt gaaagcgctc
     6301 tgtcataatg agagttaccc gataccttat tatcaacagg taactctcaa tctttaaaaa
     6361 acggctgtgt ttttaaatgt gataaacctt atacctggag tacatggaga gaataatcgc
     6421 ttatcagact tcaggtacct caaagagtgt ctttttaccg tgtcttttct gatactcatt
     6481 acaggtgtta atcatttttt tggccgcttc ttttagcaaa gaaagcatta aatcatcata
     6541 atcaacgtag aatctggttg tttcagtttc cgtttttatc tccccgatgg cggctatttc
     6601 ggtggttaat ttcaatttgg cggcatcgat atctttattc gcttgtttaa ccgtgttaga
     6661 cagggtggta aagaaactct gcacagattt taacttgttc ttcaattctg ggatcagttt
     6721 cccttcaact acgcccgcag caatagaata ggaaatgatt aatccaaatg gaccggcgac
     6781 gacaccggct gcggcaccgg catacgcttc cttcctgatt ttatctacct gtgactggaa
     6841 atagctgctt ttttctgaaa aatcattggt taactggcta tctaacgcca gcagtttccc
     6901 ggaagcgttg ttgaaacttt gtgagcttac cagcagggat ttttgcgctt cattcagctt
     6961 cgtgatgccg tcatccagta ccttaatgag aatgtctttc tgggcggatg ctttcttctc
     7021 attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca
     7081 ttcatacacc gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt
     7141 aatatcgcca actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc
     7201 ttttatggtt tcatcgaagg tctgccaggg gatgacctga tcgagatatt tattataaag
     7261 gtctaatgct ccatctgcgg tttcgattgc gtttttaact acctctaccg ttttatctgc
     7321 aacgatttca gtcataatca ttcgcctctt
//
LOCUS       DARNVM010000206         7304 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.206, whole genome shotgun sequence.
ACCESSION   DARNVM010000206 DARNVM010000000
VERSION     DARNVM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.206"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            209..598
                     /locus_tag="SI621_004283"
                     /pseudo
     CDS             209..598
                     /locus_tag="SI621_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(797..1921)
                     /locus_tag="SI621_004284"
     CDS             complement(797..1921)
                     /locus_tag="SI621_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4066925.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1921..4656)
                     /gene="rbbA"
                     /locus_tag="SI621_004285"
     CDS             complement(1921..4656)
                     /gene="rbbA"
                     /locus_tag="SI621_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEI4066926.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPTRCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSSEAEAPPVVHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSALALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4653..5720)
                     /locus_tag="SI621_004286"
     CDS             complement(4653..5720)
                     /locus_tag="SI621_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEI4066927.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(6086..>7304)
                     /locus_tag="SI621_004287"
     CDS             complement(6086..>7304)
                     /locus_tag="SI621_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HEI4066928.1"
                     /translation="NNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQF
                     ADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLGQLLNI
                     EDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQ
                     TGDRFSNICGDQFIIDLLKRIMSINKNVHVLAGNHETNCNGNYMQNFTRMKPLGEDTY
                     AGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAII
                     NKKHHTIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNG
                     NGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNKNG
                     LMPLIRRRTMLQL"
BASE COUNT         1797 a         1960 c         1774 g         1773 t
ORIGIN      
        1 tcaggctctg cgcctccatc agaccaccgt tgatcaccat atccatgtgt ttatgaataa
       61 agggcaggga aagatcacgc aacgctgctt ttttgtacta aataattcgc actaaatatt
      121 caataattga gatattcctt attacctaaa gctgttttcc attgcttata catgatcaaa
      181 tactccttac ataattaagg agaacaaaat gaaacttaaa aaattgatgg aacatatttc
      241 tattatcccc gattacagac aagcctggaa agtggagcat aaattgtcag atattctact
      301 tttgactatt tgtgccgtta tttctggtgc agaaggttgg gaagatatag aggattttgg
      361 ggaaactcat ctcgattttt tgaagcaata tggtgatttt gaaaatggta ttcctgttca
      421 cgataccatt gccagagttg tatcctgtat cagtcctgaa aaatttcacg agtgctttat
      481 taactggatg cgtgactgcc attcttcaga tgataaagac gtcattgtaa ttgatggaaa
      541 aacgctccgg cactcttatg acaagagtcg ccgcagggga gcgattcatg tcatttctcg
      601 cgtcagtcct tgcggggagc aggctttcgt aatcttgccc tgaataccgg actgagtatc
      661 aaaaagccgg ttaactgaaa ctgtccaggt tttggtggtc agttcataaa cgccttatcc
      721 ggcctaaaaa aaactaaaat tcaataaatt gcaccaatgc gtagacccta taagcttacg
      781 catcgggcaa ctgtaattac gccattgtcc caatcgtctt cctaaatcgc agcagtgcga
      841 tggtgaaaaa cgcgccgcca attgccatca gcgtcaggaa ctgcggccag acgatttcga
      901 atccggcacc ccggtagagg atggcctgcg cgaggctaac aaagtgtgtc gtcggcatgg
      961 tcagcataat gtcctgcacc atctgcggca tactttcgcg cggcgtggat ccaccggaaa
     1021 gcatttgcag cggcagcagc accagaatca ccagcagccc cagttgcggc attgaacgcg
     1081 ctatcgtccc cataaaaatg ccgattgacg tggtggcaaa cagactgagc gccacgccca
     1141 gcataaacag cgggatcgag ccttcaatcg gtacgcccag cacgcctttt accatcagca
     1201 ccagcgataa tcccgatacc accagcacca ccagccccat cgaccagatc ttcgccatca
     1261 tgatctcaaa cggcgttatc ggcatcacca gtaagtgttc caccgtgccg tgttcacgct
     1321 cgcggatcag cgccgatccg gtcaatacaa tcgccagcat agtaatgttg ttgatgatcg
     1381 ccatcacccc gccaaaccac gcgggatcga ggttcgggtt aaagcgcatc cgggtttcca
     1441 gcgataccaa cggttcgcta ttatcacggt agcgcgcgac aaagctgttc acttcaccgt
     1501 tgataatatt ctggatatac ccattgccgg taaatgcctg gctcatgcgc gtggcatcga
     1561 cgttcacctg aatatctggc tggcgtccgg cgaggacatc acgctgaaaa ttaggcggaa
     1621 tatttatcgc gaaggtatag cgtccggcgt ccagcccggc atccatctca tcggcggtga
     1681 tcatctccgg tggcaaaaac cacgggcgat agaagctgtt aacgatcctg ttcgataact
     1741 gcgattgatc catatcggca atggcgatcg gcgcgaggtt caacgatcct ggcgtaacgg
     1801 tcgctgacga atacaccgac accgtaaacg agaagacaat cagcgtcagc atcgctttat
     1861 caccgagcaa actgcgcaac tctttgatac ccagattaaa aatattgcgt aaatggcgca
     1921 tcatccctcc tgttttttca gcagcaggat gcttaagccc atcaccagcg ggatggctat
     1981 cagcaacggg ataaaaagtt gccacaaatc agtcaaatcc agcgccttcg agaacgtccc
     2041 gcgggcaata gtcagaaaat gactggtcgg gtaaacctcg ccgatccagc gtcctggacc
     2101 ttccagcgaa gctaccggat caatcatccc ggaaaactgt gtcgccggga tcaacgtgat
     2161 aatcgccgta ccgaaaatag cggcaatctg gcttttcata aaggtggaga tcagcagccc
     2221 cattccggtg gcgatgatga tatacagcag cgccgccagg gtgagcgtca ggaaactgcc
     2281 tttatgcggt acgccaaaca caaacaccga caggccgcag agcaggaaaa agttcagcat
     2341 ccccagcgcg atgtacggta gctgtttgcc aagcaaaaat tcgctacggg tggtgggagt
     2401 cacgtaaagg ttgataatcg accccaactc tttttcccgc accacgctaa gggcgcttag
     2461 cattgacggg atcatcatca gcagaagcgg gatcaccgcc ggaacaatcg ctggcaggct
     2521 ttttacgtcc gggttatagc gatagcgcgt ctcaatgttc atcagcccgc tttggctgga
     2581 gggtgtggat tgtcggctcg ccacatcctg tagccagctc tggtgcatag cctgcacgta
     2641 accttttacc gtttcggcac gactgggcat cgcaccgtcg atccagacac cgagttccac
     2701 aggcgtacca cgcgcgatat cgcgcccgaa attgggtggg atctcaatcg ccaccgtgat
     2761 atcgcccgca cgcatccggc gatccagttc gtcataactg gtgagcggtg gttgttcgat
     2821 aaagtaacgg gaaccggaga gattgagcgt ccacgcctgg ctactgacgg tctggtcgcg
     2881 gtcaagcacc gcaaagcgca ggttttccac atccatactg atgccgtaac ccattatcag
     2941 catcaggatc accgttccca tcagcgccag cgccgaacgt accggatcgc gtcgcagttc
     3001 cagtgcttcg cgacggctgt agctaaacag acggcgcaga ctaaatccct gacgcggcgc
     3061 gtgggtggcg tcgtgtacca cgggcggcgc ttcggcttcg ctgctctgcc ctgccgcttc
     3121 ctgcaaatag gcgataaatg cctcttccag actggcggct ccgcgtttct caaccaactc
     3181 ctgcggtgta ccgctggcga gcacttttcc ggcgtgcatc agtgagatac ggtcgcaacg
     3241 ttccgcttcg ttcataaagt gggtggagat gaagatagtc actttgtcct gacgcgagag
     3301 atcgaccatc aactgccaga acatatccct cgccaccgga tcgacaccag aagtaggctc
     3361 atcgaggatt aacatctccg ggcgatgaat caccgccacc gccagcgaaa gccgctggcg
     3421 aatgccgagc ggtaatgact ccggcagaac atcttcaacg tcgttgagct taaaacgctc
     3481 gctcatttca gccactcgcg cgggaatttc cgcttccggg atgtgaaaca aacgggcatg
     3541 taactcaagg ttttgccgca cggtgagttc gttatagagc gaaaacgcct gcgacatata
     3601 gcctacccga cggcgagtat cgatatcttt tggatcaacc ggttgcccga acagccacgc
     3661 ctcaccttcg ctggcgggca gcaatccggt aagcattttc atggtggtgg atttaccgca
     3721 gccgttcgaa ccaagaaaac caaaaatctc cccgcgtgga atgcggaaat tgacgtgatc
     3781 aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc gcttcgatgg caatctctgc
     3841 gttttcaggt tgatatggtg ggatcactac cgcctgatgc gcctggcgtt gcgcttgcgg
     3901 taacagattt ataaatgctt cttccagcgt ggcgctttgc gtttgctgcc gcagctcttc
     3961 ggcgctgccg gttgccagca cttctccggc attcatcgct accagccagt cgaagcgttc
     4021 ggcctcttcc atgtaggcgg tggcgaccag cacgctcata ttgctctgcc gctggcgaat
     4081 actgtcgatc agatcccaga actgggcgcg ggagagcggg tcaacccccg ttgttggctc
     4141 atcaagaatc aacagttccg ggtcgtggat taacgcacag cacagcccca gtttttgctt
     4201 catcccgccg gagagtttcc ctgccggacg atcacgaaac ggtgctaacc cggtgctggt
     4261 cagcagctca ttaattcgca cttctcgctc cgctttgtcg tgaccgaaca ggcgggcgaa
     4321 aaaatcgacg ttttcataca ccgacaaggt gtggtagagg tttttaccca gcccctgcgg
     4381 catccaggcg atgcgcgggc aaacgtcgcg tcgatgcttc gggtcgcgca tatcgccgcc
     4441 cagcacgatc acattgccct gctcaatgac acgagcaccg gaaatcaatg acaataaact
     4501 cgacttcccg acgccatccg gaccaatcag cccaaccatg cagcgggtcg gaatatcgag
     4561 agtgatattg ttcagcgcaa cggtttttcc ataatgctgg ctcacgcccg ccagttgcgc
     4621 gacaggcggg acgggaacca gttccagatg cgtcattgcg gcaacctcac caccaggtcg
     4681 tcaggccacg gaagttcttc attcacccgc acccacgcta cgcccggcaa accggttttg
     4741 acatattcca gatgctgctg gagtaattcc ggtgggatac gcgctttgac gcggaacatc
     4801 agtttcagcc gctcatcgct ggtttcgacg gtttttggcg tgaactgggc gacgctggcg
     4861 acaaaactga tggttgcagg aatacgcaga tctggcgcgg catcgaggat cagccgggct
     4921 tcaccgccca gtttcagcgt gcccgcctgt tcggttggca ggaagaaggt catatagacg
     4981 tcgctcagat cgaccatatt cagcacccga ccgcctgccg ccagcacttc ccctggctcg
     5041 gcaacccgat actgcacgcg tccgtcacgc ggggctttca gttcgctgtc atcgatatct
     5101 gcggcaatgc gccgttcagt ggcttgtgcg gcttcgacgc gtgtttgcgc ctgaatgata
     5161 ttggtgcgtg ccgcttctat agcggcttta gaagccgaaa cctgggcttt tgctgattcc
     5221 agcgcagctc gggcgctctc agcggcggcg cgatcgtcat ccagctgttg cgcagaaata
     5281 gcccctcgtt gggcgagcga acgggaacgc gtatgacgtt tggcaacgga gtccagttcg
     5341 gcctggcgtt gattgaccag cgactgtgcg gcacgagttt cgctttggcg ttgctccagc
     5401 aaagcctgcg cggcggcaac ggcgctttgt gcttctttga tttgcgcgat ggcttccagt
     5461 cgctgttcct gcaacacgcg agtatccatc ttcgccagca cttcaccttc gcgaacaaac
     5521 tggccttctt tcaccagaat ggtgtcgata cgcccggcaa ttttgctggc gatatccact
     5581 tccgtcgctt caatgcgccc attgctgacg gcaaagcctt ccggcacacc cgctgggcgc
     5641 aacagccacc acgccacgat agcggccacc gccagtaacc cgacaaccca ccacgccaga
     5701 tggcgcttac tcttatccat aatcgacccg ccataatccc tgtaagcaaa cgacgcgtgt
     5761 tacaactgcc gccgaacgac gttcagcgga ttcttccatc cgggataaga aggaaacggc
     5821 aaatacgacc agcgcgcaga gcattgagcg ccaacgtgga aggcttagtg aaaacgggta
     5881 gtaatgttgt catcatcagc attcctggcc gtaaatgaaa cacgcatact cataagtgtg
     5941 atctataaaa ctggctgaca aaacggtatc catcctgttg gatagcgttt cacgcctgtt
     6001 cagttgcagg aacagagcat tccttattac gttttatcaa cagtctcaac cagatagtaa
     6061 cattaatatt catgttgtct atggttcaga gctgtaacat tgtgcgcctt ctaatcaaag
     6121 gcataagtcc atttttgttt ttttcatgct ggtaagactt ccagggagtg gttatctcag
     6181 gccccgcacc agggttaatt tggcaattat gcatgctgaa cattcttcca gcatgtttac
     6241 tgtcacgggc gttaagacct aaaactgtcc ccgtttcacc tatgcgttct ctcccaccat
     6301 ttccgttatg tgcaacaact tgatttatac ctaacatttg tgagcatgct aatagtgctt
     6361 caagtttagg tctgtaatct gtcgaaacag aaaaataacg attaaaggat cgacaagaga
     6421 tagcccggct agttttaaac tttttaccat taattatagt atgatgcttt ttattaataa
     6481 ttgccgctaa ttcgaccgga tcgagagcat tggtcatttc atcaatagag actgttatcg
     6541 gtcctataat gtaacgtttt cgctgatcat caaaagttat cccatgatga tttgccatta
     6601 ttttgtattt ggggtcatag aaacatacag gataatcttt tatcccagcg taggtatcct
     6661 caccaagtgg tttcatacgc gtgaagttct gcatatagtt accattgcaa ttcgtttcat
     6721 gattaccagc taatacatga acatttttat taatactcat tatgcgtttt aacaaatcga
     6781 tgataaattg atcgccacag atattactaa atcgatcgcc ggtttgatcg ccaagaaacg
     6841 tacatggcgt cacagcgtct ttattaataa ctaaataagg ctgaagactt tctaaaacta
     6901 tatctcttct gttcagaata ctgattattg tattaacatt agtgtcaagt tgctcattaa
     6961 agttctccct tagcaatacg ttgagaactt catcttctat attaagcaac tggccaagga
     7021 aattttctcc ttcagccatg attcccaaat gtcccgttgc gaaaagcgcg tacaaaactg
     7081 caccaactga gccatcggta tcgccaaaat aggttggtct ggtacttgtg ggatcaacag
     7141 gtgaacaatt ggagaaatag gttctgtcag cgaactgggg ttttatcatt gtttcgaatt
     7201 taatatcaga tacattgtat tgcgatattt tccagttcca gatgggtttg tacgactcag
     7261 taactgcaac atcattagca ttatcattta ctgtcatatt atta
//
LOCUS       DARNVM010000207         7300 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.207, whole genome shotgun sequence.
ACCESSION   DARNVM010000207 DARNVM010000000
VERSION     DARNVM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.207"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            4..678
                     /locus_tag="SI621_004288"
     CDS             4..678
                     /locus_tag="SI621_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEI4066929.1"
                     /translation="MTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEGEEQISLGVR
                     NTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGPPLAKDRETL
                     FFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLRCTSLADMVM
                     VSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFPLPKGAQNSSVTYRTINDYGAETP
                     QLNCKS"
     gene            703..3303
                     /locus_tag="SI621_004289"
     CDS             703..3303
                     /locus_tag="SI621_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HEI4066930.1"
                     /translation="MPRTHRQHSLLTSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFERGQKITPGVYRVDIVLNQTMVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFDVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSSDSDSTDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSINSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNDQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMLGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGDYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDSGSQQQLNY
                     ALSGSLVAHSRGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIVLDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINA
                     AITRMDAICR"
     gene            3294..4364
                     /locus_tag="SI621_004290"
     CDS             3294..4364
                     /locus_tag="SI621_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4066931.1"
                     /translation="MQIIYGEKCVSLLRLFFAAILMLWCAQTMAYTGQCHTAQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDSWYKINDYLAARVLLQVKGNSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFIGSVVIPPMTLARLFECYNIPAGDSCTSSGDPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVKVNLSL
                     TATSDPSYPQAIKTSRPGVGIVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4376..4918
                     /locus_tag="SI621_004291"
     CDS             4376..4918
                     /locus_tag="SI621_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4066932.1"
                     /translation="MKKKTIFQYVILFFSFLNTHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4926..5441
                     /locus_tag="SI621_004292"
     CDS             4926..5441
                     /locus_tag="SI621_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4066933.1"
                     /translation="MLKRIIWILCLLGLTGSCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGMQSEMLSI
                     ESGTDAASGLAIAILDDVKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5407..6144
                     /locus_tag="SI621_004293"
     CDS             5407..6144
                     /locus_tag="SI621_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HEI4066934.1"
                     /translation="MPARHLYFIMTNTWNRLTLSIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISISLTNTSQDSWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVSWRTINDYGGLSAKKEQNLL"
     gene            6255..7265
                     /gene="pyrD"
                     /locus_tag="SI621_004294"
     CDS             6255..7265
                     /gene="pyrD"
                     /locus_tag="SI621_004294"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEI4066935.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
BASE COUNT         1940 a         1651 c         1859 g         1850 t
ORIGIN      
        1 attgtgaccg taagtttaac ggcaatatta ctcagctgtt cgtccacatg ggcggcaggt
       61 aaaggcgggg ttggacttgc agcaacacgt cttgtttatt cggaaggtga agagcaaatt
      121 tcactgggtg tacgtaacac cagtccggat gttccttatc tgattcagtc atgggtgatg
      181 acacctgata ataaaaagtc agcggacttt attattacac caccgctatt tgtgctgaat
      241 ccggcaaacg agaatctgtt acgcattatg tatattggtc cgccgttggc gaaagacaga
      301 gaaactcttt tcttcaccag cgtacgggca gtcccttcaa caacgaagcg ggaagaaggc
      361 aataccctga agattgccac acaaagcgtc atcaaacttt tctggcgacc aaaaggttta
      421 gcatatccct tagccgaggc tccggcgaaa ctgcgttgca cttcgttagc tgacatggtt
      481 atggtcagta acccaacacc ttatttcatt accctgacag acctgaaaat aggtggaaaa
      541 gtagttaaaa atcaaatgat ctcccccttt gataaatacc aattccctct gccaaagggt
      601 gctcaaaaca gtagcgtaac gtaccgaacc atcaatgact acggggcgga aacgccgcaa
      661 ctcaactgta aatcgtaagc cgtcttcagt taagagagcg agatgcctag aactcaccga
      721 caacacagcc tgttaacctc tggtggagtg ccatcgttta ttggtgggct ggtggtgttt
      781 gtgtcggcgg cgttcaatgc acaagctgaa acctggttcg atcccgcctt tttcaaagat
      841 gatccctcaa tggtggccga tttgtcccgt ttcgaaagag ggcaaaaaat aacgccaggc
      901 gtttatcgtg tcgatattgt tctgaatcag acaatggtag atacgcgcaa cgttaatttt
      961 gttgagataa cgccagagaa ggggattgcc gcctgcttga ctactgaaag cctggatgca
     1021 atgggcgtga atactgatgc gtttccggct tttaaacaac tggacaaaca agcgtgtgcg
     1081 ccattggcag agattattcc ggatgccagc gtaacctttg atgtgaataa actccgtctg
     1141 gaaatttcag taccgcaaat tgctataaaa agtaacgccc gtggctatgt tccccctgaa
     1201 cgctgggatg aagggatcaa cgcactatta ctgggatatt cttttagcgg ggccaacagt
     1261 attcatagca gttcagatag tgattccaca gatagctatt ttttgaattt aaacagtggc
     1321 gtgaatttag gtccgtggcg attgcgcaat aattccacgt ggagtcgtag tagcggccaa
     1381 accgcagaat ggaagaatct cagcagctat ttgcagcggg cggttattcc tctgaaaggc
     1441 gaactgaccg tcggtgatga ttataccgca ggcgattttt tcgacagcgt cagctttcgt
     1501 ggcgtacagc tggcgtcaga tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct
     1561 gtggtgcgtg gtatcgccaa aagcaatgcc cagataacga ttaagcaaaa cggctacacc
     1621 atttaccaaa cttatgtctc gccaggcgct tttgagatta gtgatctcta ctccacgtcg
     1681 tcgagcggtg atttgctggt tgaaataaaa gaagctgacg gcagcatcaa tagttacagc
     1741 gtgccctttt ccagtgtgcc attactccag cgtcaggggc gaatcaaata cgcggtgacg
     1801 ctggcgaaat acagaaccaa tagtaatgat cagcaagaaa gtaaatttgc tcaggccacg
     1861 ctgcaatggg gcggaccgtg gggaacgacc tggtatggcg gtggacaata tgctgaatat
     1921 taccgtgccg ctatgttggg gctgggtttt aacctcggcg atttcggagc gatttcgttc
     1981 gacgcgaccc aggcgaaaag tacactggca gaccaaagtg aacataaagg gcagtcatat
     2041 cgttttctgt atgccaaaac gctcaaccaa ttgggcacta actttcaatt gatgggctat
     2101 cgctattcga cgtcaggctt ctacaccctt tccgacacca tgtataaaca catggatggc
     2161 tacgaattta atgacggtga tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg
     2221 ttttacacca aacgtggcaa actgcaggtc aatatctccc agcaattagg cgattacggt
     2281 tcgttttatt taagtggtag ccagcaaaca tactggcata ccgatcaaca ggatcggcta
     2341 ttacagtttg gctacaacac gcaaattaaa gatctctcac tgggcgtttc ctggaactac
     2401 agtaagtccc gtggtcaacc tgatgctgat caagtatttg cacttaattt ttccctgccg
     2461 ctcaatctgt tgctccccag aagtaatgat agctatacca ggaaaaaaaa ttacgcatgg
     2521 atgacctcta ataccagtat cgataacgaa gggcacacta cacaaaatct tggtttaacg
     2581 gagacactgc tcgatgacgg taacctgagc tacagcgtgc aacagggata taacagcgag
     2641 gggaaaacgg cgaatggtag cgccagcatg gactacaaag gggcgtttgc ggatgcccga
     2701 gtgggctaca actacagcga tagcggtagc caacaacaac ttaactacgc cctttcaggc
     2761 agtttagttg cccattcacg gggcattacc ctggggcaat cactggggga aaccaacgtt
     2821 ctgattgcag caccaggcgc ggaaaatact cgtgtggcga atagcaccgg gctgaaaact
     2881 gactggcgcg gatataccgt tgtcccctat gccacttctt atcgtgaaaa tcgaatcgta
     2941 cttgatgctg catcgttaaa acgtaacgtg gatcttgaaa atgcagtagt aaatgtagtt
     3001 cccaccaaag gggcgttggt tctggcggag ttcaatgccc atgcgggggc gagggtatta
     3061 atgaaaacat caaagcaggg tataccgctg cgatttggcg cgatagcaac gctggatggg
     3121 gtacagacca atagcggcat aattgatgac gatggctcgc tctatatgtc cggtttgccc
     3181 gctcagggag cgataactgt acgctggggt gaagctcccg atcaaatttg tcatatcagt
     3241 taccagctta ccgaacaaca aattaacgct gcgattacgc gaatggatgc catatgcaga
     3301 taatctatgg agaaaaatgc gtgtcattac tacgactatt ttttgccgcc atcttaatgc
     3361 tatggtgcgc tcaaactatg gcttataccg ggcagtgcca taccgctcag gggaatccgt
     3421 atattggcgt caattttggc gttaaaaccc tggatgaaga agaaaatacg gccggagtag
     3481 ttaaagacaa attttatcag tggaacgaat cgaatgatta ctatgtttct tgtgattgcg
     3541 ataaagacaa tgtcaggagt ggcagatggg cattcgctgc ggattcaccg ttagtctatt
     3601 taggcgacag ctggtacaaa attaatgact atcttgccgc cagggtttta ttacaggtta
     3661 aaggtaattc tcctacggcg gttccattcg aaaacgtggg caccgggggg gatacccgat
     3721 ggcatatttg cgatcccggc ggtcaacgtt taggcggcca gggggctagc ggtaatagcg
     3781 gtagcttttc cctgaaaata ttgcagccgt tcattggctc ggtcgtcatt cctcctatga
     3841 cgctggcacg attgtttgaa tgctacaaca tacccgcagg ggattcctgt acgtcgtcag
     3901 gcgacccggt tttagtgtat tacctgtctg gcacgatcaa ttcacttggc tcatgttccg
     3961 tcaatgccgg agagacaatc gaggtcgatc tgggcgatgt atttgcggct aattttcgtg
     4021 ttgtagggca taagcctctt ggggccagaa cggctgaact cgcaatccct gtcaggtgta
     4081 acactggaaa cgctgggtta gttaaggtca acctgagtct gacggcaact tcagacccca
     4141 gctatcccca ggcgattaag acttcacgtc ctggcgtggg cattgtggtg accgatagcc
     4201 agaacaacat tatttccccg gcgggtggaa cactaccgct ctctattcct gatgatgcag
     4261 acagtatcgc gcgaatgaat gtctatccgg ttagcacgac aggcgtacca ccagaaaccg
     4321 ggcgatttga agccacggca acggtgagaa taaattttga ttaacgcgtg aacgtatgaa
     4381 gaaaaaaaca atatttcagt acgttatctt gttctttagc tttcttaaca cccatgtcgg
     4441 gatggctggg cctgaacaag tcagtatgca tatttatggt aatgtggtcg atcagggctg
     4501 tgatgttgcc accaaaagcg cattacaaaa tattcatatt ggcgatttta atatcagtga
     4561 ttttcaggct gcgaataccg taagcacagc tgctgatttg aatatcgata tcaccggttg
     4621 tgccgctggt attactggcg cggacgtcct ttttagcggc gaggctgaca cccttgcgcc
     4681 gacactactc aaactaactg acacaggcgg aagcggtggt atggcaacgg ggattgccgt
     4741 gcaaattctt gatgcgcaaa gtcagcaaga aatcccgctc aatcaggtcc agcctcttac
     4801 gcccttaaaa gcgggggata acacactcaa atatcaactt cgttataagt ccacaaaggc
     4861 gggggctacg ggcggtaatg cgacggcggt tctctatttt gatctggttt accagtgaag
     4921 gagtgatgtt gaaacggata atctggattc tgtgcttact gggtttaacg gggagttgtg
     4981 agctatttgc ccatgatggc acggtcaaca tcagcggatc gtttcgccgt aatacatgcg
     5041 tgctggcaca ggatagcaag caaatcaatg tgcagttagg cgatgtctcg ctgacacgtt
     5101 tttctcatgg gaactatggc ccggagaaat ctttcatcat taatttgcag gattgcggaa
     5161 cagatgtcag cacggttgac gttacctttt caggaacacc cgacggaatg cagagcgaaa
     5221 tgctgtccat tgaaagtggt acggatgcag ccagtgggct ggcaattgct attctggatg
     5281 atgtgaaaat attgattccg cttaaccagg cgagtaaaga ctacagccta catagcggta
     5341 aggtgccatt aactttttat gcgcaattgc gacctgttaa tagcgatgtg cagtcgggta
     5401 aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa cagattgacg
     5461 ctctcgattt ttgccgtttt atcgctgctg gtggcggggg aattgcaggc aggagtggtg
     5521 gttggcggaa cgcgatttat cttcccggca gacagagaat cgatatctat ttcactgact
     5581 aataccagtc aggattcctg gcttattaac agtaaaatca atcgcccaac gcgttgggcc
     5641 gggggggaag cgtcgacagt gccagcacca ttactggccg ctccgccact tattctcctg
     5701 aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg
     5761 gatcgcgaaa cgctatttga gttaagcatt gccagcgtac catccggcaa agttgaaaat
     5821 cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc cgaaggtttg
     5881 ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc gcagggtgta
     5941 caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt aaatggtaaa
     6001 gcgttaagta atgcgggagt tgtgccacct aaaagccagc gtcagacaag ctggtgtcag
     6061 gcaatagcac cgtgtcatgt ctcctggcgg acgataaatg attacggcgg gttgagtgca
     6121 aagaaggagc aaaatctgct ctgaaacagg ctcggaaaac gtttgcgctt tttttgccac
     6181 aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg cacaccggga
     6241 atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag ctcgatccag
     6301 agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg ccgtttgaag
     6361 cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg acgtttaaaa
     6421 atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac gcgttaggcg
     6481 cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag ccaggtaatg
     6541 acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt atgggcttta
     6601 ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa agcccattat gatggcgtcc
     6661 tgggtattaa catcggcaaa aataaagata cgccggtgga gcagggtaaa gatgactatc
     6721 tgatttgtat ggaaaaaatc tatgcttatg cgggatatat cgccatcaat atttcatcgc
     6781 cgaatacacc gggattacgt actctgcaat atggcgaagc gctggatgat ctcttaaccg
     6841 cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg ccgatcgcag
     6901 tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat agtttagttc
     6961 gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct ctggttcagg
     7021 gaatgaaaaa ttgtgatcaa accggtggct taagtggtcg tccgcttcag ttaaaaagca
     7081 ccgaaattat tcgccgttta tcacaggaat taaatggtcg cttaccgatc atcggtgttg
     7141 gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggggcc tcactggtgc
     7201 aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc gttacccata
     7261 tctaattatt tcttcgaatt cctctttata agataatcag
//
LOCUS       DARNVM010000208         7277 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.208, whole genome shotgun sequence.
ACCESSION   DARNVM010000208 DARNVM010000000
VERSION     DARNVM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.208"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            1..496
                     /locus_tag="SI621_004295"
                     /pseudo
     CDS             1..496
                     /locus_tag="SI621_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            576..868
                     /locus_tag="SI621_004296"
                     /pseudo
     CDS             576..868
                     /locus_tag="SI621_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(908..1942)
                     /locus_tag="SI621_004297"
     CDS             complement(908..1942)
                     /locus_tag="SI621_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HEI4066936.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(1945..2910)
                     /locus_tag="SI621_004298"
     CDS             complement(1945..2910)
                     /locus_tag="SI621_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEI4066937.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(2967..3725)
                     /locus_tag="SI621_004299"
     CDS             complement(2967..3725)
                     /locus_tag="SI621_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEI4066938.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(3739..4953)
                     /locus_tag="SI621_004300"
     CDS             complement(3739..4953)
                     /locus_tag="SI621_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEI4066939.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(5149..5509)
                     /locus_tag="SI621_004301"
                     /pseudo
     CDS             complement(5149..5509)
                     /locus_tag="SI621_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5571..6261
                     /locus_tag="SI621_004302"
                     /pseudo
     CDS             5571..6261
                     /locus_tag="SI621_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6497..>7277)
                     /locus_tag="SI621_004303"
     CDS             complement(6497..>7277)
                     /locus_tag="SI621_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEI4066940.1"
                     /translation="NDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPDYPALAVKYLAQ
                     ITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASGPRCGLAEIN
                     LPQLQPGSSIMPGKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVMEPVITFA
                     RFTSLKVMTNACNTLRTKCIDGITANSDRTAEMVMHSCGIVTLLKPHLGYKVCSEMAH
                     EAYHTGKSLHQIVVVERKLLTQEEWEKTFNLDNLIAPKFEQ"
BASE COUNT         2022 a         1644 c         1678 g         1933 t
ORIGIN      
        1 caggggggtt acaggcagtg aaaccatcgt cagggatgct gttgccaaat ggcataaagg
       61 ctggatccca ccggttacta ctgccgcaag acttccttca gtgtcccggg taagccgctg
      121 gttgatgccc tggagaataa tcaggggtga agaaaattat gctttccgat ttattagtct
      181 gatgtgtgaa aaagaaccgg agttgaaaat agcgcagcaa ctggtactcg agttctaccg
      241 tattctgaaa acctaaaata aatcacagct aagcagctgg ttcactcggg tccacgaaag
      301 cggctcagca gaacttcggt gcgtagctgc ggggatggaa gctgatgctg cggctatatg
      361 tgaggcaatc agcagtcgct ggagtactgg tgttgtcgaa ggtcatgtaa atcgcctgaa
      421 ggtgctgata cgccagatgt atggtcgagc cggacttgaa ctacttaggc ggagggtaat
      481 gagtccactg gcatgaaaat cttcacctag gttgaggaag agccactttt gcactgttcg
      541 tgacagtatg gcctttatgt actcactttg ttaccggtag ctggatcatc actaccgtgc
      601 cgcaaatggt ggtgaacagc atcaattgtg gaatgaatgt tgccgaagcg accaatgcgc
      661 ctcgtttcca ccatcagtgg ttgccggacg agctgcgtgt tgaaaagggt ttagtccgga
      721 tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaatgagg cgatgggcag
      781 cacgcaaagc attatggttg ggtcggatgg tgagttgtac ggcgcatccg actcgcgctt
      841 ggtggatgat ttaacggcgg ggtattaatt gacatccccc ggcgcaatgt gccgggggat
      901 aatggtttta tgcggtcaat aatcgtccgg gattatcgat acagagtttt ttgaggatcg
      961 ttttatcgac gccgcgctcc gccagatagg ccagaaaaac atctggaaca aatccccagc
     1021 cattaccgcc atttttagcc cacatttgtt taaggaagac atcgtggctg agaacaagct
     1081 gatcggcgta tcccaactca atgagatgag cgacggcatc ggcagtctcc tgcacccctg
     1141 gcgctattcc ctctttcggg aaggtaatgt ctaggccaat catgtcgaat tccagccaga
     1201 cacctttatc aagcattttc cgctgatacg ccacgtcttt tcctgacgga tccgagtgtg
     1261 cgagagagac tttatttggc gagacgccca tttcacctaa cacaatgtcg agtacttcat
     1321 caccgcgacg aagccagccc ggcatgtgaa tattcatcgc cacatgagga ttgttaatct
     1381 gtgccagcga ggcagcccgc aagctgttat gctcggcttc ggtaaatgtc ggtgagacac
     1441 ctatttcacc gatcattccg gcacgaatat ccgtatcgcc aatcccttga ttcaattctt
     1501 tatcgatcgt tgtcgctagt tcatcaaccg ttttatgaat tctctggctt tcaaattttt
     1561 ccaggtaggg accggaggaa gcaacaatat tcagacctgt ttttaatgcg acttctcgta
     1621 atgcctgcgc atctcgtccg atagactcag accctgtggc atcaacaatg gtgcgaccac
     1681 cgagcgagat aaagttatta atttcgaaga tcacatcttc aataggtttg cggtccatat
     1741 tatcagcgca gcaataagga tcgtgtttta atgcccactg gatttctgcc gtgacttttt
     1801 tatcaacaag ccgttgtgaa aaaggatagc aaggagcatc cacaacagag gaaagatcgt
     1861 tgaacagatg ttcatgagga agtgttagcc ccatatcttc acgggcgacg gggcccgtca
     1921 cagtttgtag ataatctttc atgattatac ctccattttc ttcattgttt tcgaggctct
     1981 gtattccgga atagtggtaa caattgcacc gaccagtgca aacagtgttc cgatgatcgt
     2041 gaccagataa acggtattgc ccagcgatgg aattaatacg tcaatcaata ctgatcccag
     2101 taactggccc gccgttgaag caacccccag catcagtaac ccaagacctc tgaccaaaat
     2161 ggccattaaa ccgatagaca ttagtccaag cgggccgcca agatacatcc accagacatc
     2221 tggtaattga acagtgacgt gccctaatgc gacacgaata gctagcgctg tgcctaaaac
     2281 acaaaatcca acgatgaaat tccaggtgat tgaaaccagc atggatcccg tagcttcagc
     2341 tacttttgca tttccagcgg gctgccatcc tgcaagcaaa ccagctaaga acggaagaat
     2401 ggcaagtaaa atgaaagagg tagaatgcca ctgcggtgac acaacaaaaa tcgtagcaat
     2461 aaccgcaaat aatgcaccga tgatgcgata aggggtaaag tattttttct cgtcgacacc
     2521 gataccaaag cgatcgcaaa gtaaaccaga cagcaatagc gcggagatca gtgcggtctg
     2581 aaacgttgcg accccgagtg cgctggctga tgcgccttca gaaaatacca ccatcgctcc
     2641 acagagaccg gcaaaccagt tccagagagg aacttttcgg tttttaatca gagaaggaat
     2701 tgccgcaaat tgttgccggg tttcttttcg cgccataata ataaaaaaca tcacgactaa
     2761 accactggca aaggagataa ctgcgcatgc attaccgtcc tgtaaccagt gacctaattg
     2821 accattcacc gctgattgca taggggagag catacccgca agaatcgtgg caagcatcca
     2881 taagggagtt gagtacttat tctttttcat acatcaatcc ttctattata gtaaatggag
     2941 taactggtgt taccccatta ttattattat cttgcaatat gagtcagtat atttacccac
     3001 aacgcggtat agaacggcca ggacataaac tgctgtgtac cccagtgcgg tgaacagtca
     3061 ctcataaagc aggctatttt cccgttgtga tagttaccaa agaccagtaa tggatcgttg
     3121 tttattgtta ataccacctc agcattttct ttagcgatcg cccggttata acctaggaaa
     3181 aaaggatatt cagagaaccc tttaataacc ggatggtcag gttgtgatgg ctgtgcaata
     3241 acaccctctg gtttttcgac acgatcgtca ccatctaaca tcgtaaccgg gagcacatca
     3301 gccagaactg tatttttata attggctttt gcttcaattc ccatgaatga gagatagccg
     3361 ccaatcatca ataacccacc accgttattc acatattctt taatcagttc gagagcgttt
     3421 ggttttatgc gaagctgata aaaagtatca ttttgtagca ggaaagtatt actaccaata
     3481 tcactaataa cgatggcatc atattgttct aactgtgcca catcttcagg gaaggcaatt
     3541 tgtaccgtat gtgccggcat ataggtgaca tctacctgac tatttttcag acattggagt
     3601 aaccaggtcg caccttcttc atatttactt gaggtaaaac tgtcatatcc ttttgaatga
     3661 atcatatgaa tatgccagga ttctccaata aagagcacct ttagtgattt tttggtgtta
     3721 ttcattctct gtcccttctt aatgcgtaaa aatagtttgc tgataatgta tactctgaat
     3781 tcgatgtatc acagattcat gttcaggcat gtcggaagca ttgcttgtct ccaccgccag
     3841 ggatgcaaag gcgctggcat aacacagtgc tgattccagt gattttcctt tcgcaagtcc
     3901 tgatgccaga gcgccgttaa aagcatcccc ggctcctgcc gtatttttaa cgaccgctgg
     3961 aaacgctggc gaatgaatga attttttgcc atcaaatgcg agtgagccct tgctaccgag
     4021 cgtgataacc gtgtttttta cccctttatt gtggatagct tctgcagctc gtttagctga
     4081 ttcaagatcg tgtacgtcta tgccggacag taaaccagct tctgtttcat tgggggtgat
     4141 ataatcaaca tcctgaataa gttcgttgac gatatcatta tagggtgccg ggttaataat
     4201 gaccgggata ctgttttttt gcgccaatgt gatcgcttgt tgcagagcgg tataatttgt
     4261 ttcgagctgc agaagtatga catcagaatt aataatcgct tctttctgaa tagttatttc
     4321 atcggacgac attgtcatgt tggcaccggg atagatcgca ataacattat cgccagtacc
     4381 ttcattaaca aggatcgtgg cagttccggt ttgcgtttct ttcgtttgat aaattatact
     4441 tttataaatc ttagacgaat tcataaagtt gatcgcataa tcactaaagt gatctgtacc
     4501 tacttttgtt atgaaataaa catcagtatc ggcaaagctt gctgccagcg cctggttaca
     4561 accttttccc cccggtgaaa atataaaatt acttgccaga agcgattctc cgatagtggg
     4621 aagcctcggc aaataactga tgatatcaac attaaacgaa ccaataacgc atacttgact
     4681 tttcatagtt gagtggcctt tttttattcc ctgggctgta tcataatttt caagcgcata
     4741 agcgatttca tttttcgcaa tagtatcaag cgagtctagg acgataaaag caccgccatg
     4801 acatcgagtc agatagccaa gtctggcaag ttcattaaga tctgatcgaa tagtttcttt
     4861 cgttacattg aggctattgg acagatcgga tacttttaca gaaccgaatt tactgagtcg
     4921 tttcaatatt tcatgatggc gttcaaattt catgcttcac ctcacttcag ttacgttaat
     4981 atctgtcgat gtatattgat gtgataatca ccataaagtg gcggggaaaa gcaaaaaaaa
     5041 tcaaaaatag ccggagaaaa ggtaagcgtt aatggagaat catattgatg cttatttatt
     5101 ggtgagaact acgttccatg gcaggagttc atcaatacgg ttggaaggtt attccggcag
     5161 tacgctcagg atatggcgca gatactcttc cggatcaatg tcgttcagaa ggcaagtgcc
     5221 gataagccca tacaacagtg cactgcgctc gccgtcgaag aacataaagt ttttatttcc
     5281 gagacagatg gcacaaaggg ctttttccgc ggcattatgt ctgcctccgc ctgaccgtca
     5341 acactgtaat aacagagtgc gccccactga ttcagtacac agctgaacca ttctcccagt
     5401 ctggattttt ggacagcgtg gcatttttct ccaccatcca cgcatgcagt gagtccagta
     5461 acagtttgct cttcctctgc ctgaccacaa gatgttctgg ttctggcata ctgaatttgg
     5521 ccacctgagc ataggtaata tgctcacctt agaacaacac agatgctcca atgaaaaaag
     5581 gaaatttcag cgcagagttt aaacgcgaat ccactcaact agttgttaac cagaacttta
     5641 cggtggcagg taccatccaa agctatggat gtcggtcttt ccacaatgac aagataagtc
     5701 aaacaactgc gtgatgagca tcagagcaaa acaccaaaag cctctctgat gacaccagaa
     5761 caaatcgaaa tacgtgagct gaggaaaaag cggcaacgta ttgaaataga gaatgaaata
     5821 ttagaaaagg ctacggcgct cttgatgtca gactccctaa acagttctcg ataatcgaga
     5881 agctcagagc gcattatctg gtgatcgcac tttgccatgt gtgcaggatt catcgaagca
     5941 gctacaaaga ttggaaaaac cgtcctgaaa aaaagacggt agacgggctg tattacgtag
     6001 tcaggtaatt gagcttaatg gcggagcaag gaacatcgcc acaattgcaa cccggagaga
     6061 ctaccagatg ggacgcaagc ttgctggcag tctcatgaaa gaacaggggc tggtcagctg
     6121 tcagccgttg accctccggt acaggcatgg cagtcatgaa cttgtagcta tcctaagaac
     6181 ttgaagggcg cttagctgtg acacagctaa atcaagtatg gtgacgtgac ctatatctgg
     6241 actggtaaac gatggggata cccgcttacg cgagagcaat aagtggctgg ccaccattct
     6301 cgatacaaaa ctctgctgaa tagcagcgat tttcccagcg tggtgcgtct tttgcatttt
     6361 actggttctg agttatcaat tatctgttca ctaatgacta ctctaaaaag ttgtatcaat
     6421 ttaagtgata tagggagtct ggagattcag ggggccagcc taggaacatt ggttacattt
     6481 ttagtttcca aacattttat tgttcgaact tcggagcaat cagattatcc agattgaatg
     6541 tcttctccca ttcttcctgt gtgagtagct tacgttcgac aaccactatc tgatggaggg
     6601 atttgcctgt atggtatgct tcgtgcgcca tttcagaaca caccttatat cccagatgtg
     6661 gcttcaggag agtcacaata ccgcaggaat gcattaccat ctctgcagtt cgatcggaat
     6721 tagctgtaat gccatcaatg catttagttc ggagtgtgtt acaggcattc gtcatcacct
     6781 tgagtgaggt gaaccgcgca aaggtgatga ccggctccat aacgttaagc tcaagctgac
     6841 cagcagatgc cgccagcatc actgtggtgt ccagaccctg aaccaggaag caagactggt
     6901 ttgttacttc cgggataacg gggttaacct taccaggcat aatggaagaa cccggttgca
     6961 attgaggcag gttaatttca gccaggccgc aacgggggcc tgaagcaagc agtcgaatgt
     7021 cattacaaac cttggtaagc ttcacagaga ggctcttcat ggccgaactg agtgcaacat
     7081 atgcaccaca gtcgcttgtg gcagcgatga gatcttcact gttcctgaaa ttaatgcctg
     7141 tgatctgggc cagatatttc acggctaaag cgggataatc cgggtgagcg gtaacacagg
     7201 taccaatcgc agtagcacca aggtttacaa cccgcaggtg ttcctgtgca ttacggatga
     7261 cccgaatttc atcatta
//
LOCUS       DARNVM010000209         7155 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.209, whole genome shotgun sequence.
ACCESSION   DARNVM010000209 DARNVM010000000
VERSION     DARNVM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.209"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(142..1620)
                     /locus_tag="SI621_004304"
     CDS             complement(142..1620)
                     /locus_tag="SI621_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEI4066941.1"
                     /translation="MERYFIGVDLGGTETKAGIYTAEGREIGVSSQVLPLLSPCPGFC
                     ERDMEALWKVTCQVISLSMSENNITASQIDGISFSAHGKGLYLLDRQGKPLRHGIVSS
                     DSRAQQLVASWKEQGIDRESYHRSLQQLWPSHPVALLHWLKKHETDNYGRIGTILMVH
                     DYIRSRLTGKLACEETNISGSNFYNQYTSAYDAELFRLFEIKEVAGKVPDIIGSADLA
                     GVVTSEAASQCGLLAGTPVFGGVFDVVGAALTSGIYDSTTLSAVAGTWSIATRIFDRI
                     IPSDYPYVWGKFSIPGTFFAHEGSPTSASNLTWFLQQFLPDLPDSYRVINEWVERGYE
                     KQNDILFFPWLFGSNYHEALSGGFLGLCGHHKREDLVYAIYQGIVFSHLLHQDRLIEI
                     DKSLQTIRFSGGPTHSRIWMQMFCDASNMPLEIVDIEQSGCRAAAMCAAVGGGAYSGF
                     KQAMKATHLNVIRLEPNVEKAHMLRRRFARFKQVASALSSVV"
     gene            complement(1637..2500)
                     /locus_tag="SI621_004305"
     CDS             complement(1637..2500)
                     /locus_tag="SI621_004305"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEI4066942.1"
                     /translation="MRRHPLGIYEKALPRNTSWVEKLALAKSCGFDFVEMSVDENDER
                     LARLDWSLTERMELISAIQQTGIRIPTMCLSAHRRFPFGSHDRDTRQQARTIMLKALR
                     LAQDLGIRTIQLAGYDVYYEPQNEETIAWFEDAMAWTAEQAAAAQVMCAVEIMDTPFI
                     NSISKWKVLDTKINSPWFTVYPDVGNLTAWGNELESELVKGIDRIAAIHLKDTYPVTP
                     DSPGQFRDVPFGEGCVDFIKLFKILRNLNYRGTFLIEMWTEKATEPLLEIIHARQWIE
                     EKMRLAGWDNI"
     gene            complement(2500..3546)
                     /locus_tag="SI621_004306"
     CDS             complement(2500..3546)
                     /locus_tag="SI621_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4066943.1"
                     /translation="MNKRIFFPLDGTRSGLLSICFIVVVLFSLAMPGRFFTDNTFLSI
                     AFQLPELGLLTFAMFIPMLSGGLNLAIIGTANLTSLFMAWVLIQYVPADASTGSQLFW
                     LFLAIVGAMLIAIIIGAITGLIISHIGAHPILVTLGSMTIISGIGIYLTKGAALSGMP
                     PVVRSIGSEVIMGIPVPMMIFIVTTVALALFLGKTRTGKTIYMCGSNINATWFSGIRT
                     DRVLIAIYAISSMLCVLAGLIMLARFNSARMGYGDSYLLLTVLAIVLGGTDPFGGAGK
                     VSHVFCALLVLQVIATGLSLLGLSLHFNLAVWGITLIAALAFKFIKQKWQLQRDMNKS
                     RKIFQQQLITREGK"
     gene            complement(3560..4552)
                     /locus_tag="SI621_004307"
     CDS             complement(3560..4552)
                     /locus_tag="SI621_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4066944.1"
                     /translation="MVSFMRLRFPRSVEAWLGWVIVLMLVFFSIMSDEFLSIQNLLDL
                     SESYAVTGIFALGLFVVLVTGGIDISFAAVASVVQYVVASLLLNETLSNPFFCLVFAV
                     FFGIIFGLINAILIYYLNVVSIIITVSMQSLLFGMLMWLTNGHSIYDLPDWWITQRSF
                     LSFEFGGEDYQIGLPLVVMLGMAFLTWVIMNKTHIGRQLYAVGGNTESARRIGIRVSL
                     IYLFAYGYLGAAAAIGGMLQAYRMSEVVPNALVGGELDVLAAAVLGGASLSGGRGTVI
                     GTLMGVFLIGILKNGLNLIGVSSYFVNIVIGVVIVSAICVTHYKKRKETDVGFV"
     gene            complement(4545..6059)
                     /locus_tag="SI621_004308"
     CDS             complement(4545..6059)
                     /locus_tag="SI621_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEI4066945.1"
                     /translation="MPHIIEKNAEQPLVTLRSLTKSFGGHRALKNINLTLNKGEVHCL
                     AGANGCGKSTLIKTLCGVYVPDSGSEIIIDGKSWTRLSPDKARELGIQVIYQDLSLFP
                     NLSVAENIAFEYNLKGYLGLHRKKQLRDVSQKIIQELEFNLDPDALVQHLPIAQRQQV
                     AICRALIADARLVIMDEPTASLTRTEVNQLLRTVNYLRDKNITVVFVSHRLDEVKEIS
                     DRITVIRDGEIIGTWAANQLSPGDITELMTGKTIVHQKKLPNSVEGKVVLELKKLCRK
                     GQFEDVSLKLHQGEVLGLCGLLGAGRTELALSLFGITRADSGDIYLEGKPVTIKDNTH
                     AVKLGIAYVSEDRLTLGAVLPQSISDNMVLSILDRLKTPSGFIDENRKETLVKEWIQD
                     LDIKVTDPNNPLSTLSGGNQQKVVLAKWILTRPKVLILDAPTVGVDIGAKDSIYQLIH
                     RLSGVGISVILITDEAPEAYYNCDRIFHMKRGKIIHELLPSNYTEQQLAEVING"
     gene            complement(6126..7130)
                     /locus_tag="SI621_004309"
     CDS             complement(6126..7130)
                     /locus_tag="SI621_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEI4066946.1"
                     /translation="MKKLILPCLIATVLSSTAAWADQTVTSQKANKPFTMGVVVKVGG
                     IPWFNVMEQGIKDEGKLLGVDAWQVGPTTADPAEQVRAIEDLIARKVDVIGVVPNDAK
                     VLEPVLKRAQEAGIKVITHESPDQKNADWDFELLNTQTMGANHMKDMAKCMGEEGKYA
                     MFVGSLTVPLVNDWANAAIAYQKEHYPNMTLVEDRFGVAESVDDSMRTTNDLMSKYKD
                     LKGIMSFGSQGPIGAGRAIDKRKKNDAICVFGTFTPGQGIKLLEKGAIDGGYISNPMI
                     AGKVFVQVATAMMNGEPIKDGVKIGDMGEIKVNNNTIYSDNPEKLDLENTRHLVKLGL
                     "
BASE COUNT         2140 a         1808 c         1332 g         1875 t
ORIGIN      
        1 tggtcgcggt aagggtagcc gttcctccct tccgcaaccc attaaccctt gaagtcacag
       61 tctatttcag gcatttctca actcaaaata acgaaggcca atgtgaactt tgtggctctc
      121 cagaatccgc tttgttttcc atcaaacaac ggaactcagc gcactggcaa cctgtttaaa
      181 gcgagcaaac cgtcgcctga gcatgtgtgc tttttctaca ttcggttcaa gacgaatcac
      241 gttgagatgc gtggccttca tggcctgttt gaatccagaa taagcccctc ctcctactgc
      301 cgcacacatt gcagccgctc tacaacctga ctgttcaata tcgacaattt caagaggcat
      361 gttgctggca tcacaaaaca tctgcatcca gattctggaa tgcgttggcc cgccagagaa
      421 acgaatcgtt tgcaatgact tatcaatctc aataagacgg tcctgatgca acaaatgaga
      481 aaagacaatg ccctgataaa tcgcgtacac caaatcttct cttttatgat gcccacacag
      541 ccccaaaaat cccccgctta atgcctcatg gtaattcgag ccaaacaacc acgggaaaaa
      601 gagaatatca ttctgcttct cataccctcg ttccacccac tcattaatca cccgatagct
      661 gtcaggaaga tccggtaaaa attgctgcaa gaaccatgtc aaattactgg ctgatgtagg
      721 gcttccctca tgggcgaaaa atgtacctgg aatgctaaat ttcccccata catagggata
      781 atcagaagga ataatgcgat caaagattcg cgttgcaatt gaccaggtgc cagcaaccgc
      841 acttaacgtt gtactgtcat agatgccaga agtgagcgcc gcaccaacga catcaaaaac
      901 accaccaaaa acaggcgttc cggcgagaag cccacattgg ctggctgctt cagatgtcac
      961 aacccctgct aaatcagcgg agccaataat atcgggaacc ttacccgcaa cttccttaat
     1021 ctcaaatagg cgaaaaagtt cagcatcata agcactggta tattgattat aaaaattact
     1081 gcctgaaata ttggtttctt cacaagccag tttgccagta agccgtgaac gtatgtaatc
     1141 atgaaccatc aaaatggttc cgatgcggcc ataattgtca gtctcatgtt ttttcaacca
     1201 atgcaatagt gctacagggt gcgatggcca gagctgttgc agactacggt gatagctttc
     1261 gcggtcaatt ccttgttctt tccagcttgc aaccagttgt tgtgcgcgag aatcagagga
     1321 aacaatgccg tgtcgtaagg gcttaccttg ccggtcgagc aggtacaaac ctttaccatg
     1381 agcagaaaaa ctgatgccat caatctgact cgcagtaata ttgttctctg acatcgacag
     1441 tgaaatgacc tggcacgtta ccttccataa cgcctccatg tcgcgttcgc aaaaaccagg
     1501 acatgggctt aacagaggta acacttgcga agagacacct atttcacgtc cttctgccgt
     1561 ataaattccc gctttggttt ctgtaccacc taaatcgaca ccaataaaat accgctccat
     1621 gacccctcct tagtttctaa atattatccc agccggcaag gcgcattttc tcttcaatcc
     1681 attgacgtgc atgaataatt tctaatagag gttccgtcgc tttttctgtc cacatttcta
     1741 tcagaaacgt tccgcgataa ttaagattac gcagaatctt aaataattta ataaaatcta
     1801 cacacccttc accaaaagga acgtcacgaa attgccctgg agaatcaggc gttactgggt
     1861 acgtatcttt aagatggatt gcggcaattc gatcaatccc ctttaccagc tcactttcca
     1921 gttcattacc ccatgcggtc agattgccaa catccggata aacggtaaac caaggggaat
     1981 taatttttgt atccaggact ttccatttac taatagaatt aataaatggc gtatccatga
     2041 tttctacagc acacataact tgtgccgctg cggcctgttc agctgtccag gccatcgcat
     2101 cttcaaacca ggcgatagtt tcttcattct gcggctcata ataaacgtca tagcctgcca
     2161 gttggattgt tcgaatcccc aaatcctgcg ccaggcgtaa cgccttcaac ataatcgtac
     2221 gcgcctgctg acgagtatct ctatcatggc taccaaatgg aaaacgccgg tgagcagaca
     2281 ggcacattgt cggaatacgg atccccgttt gctgaatggc gctaataagc tccatacgct
     2341 cggtgagcga ccagtccagg cgggcgagtc gttcatcatt ctcatctacc gacatctcaa
     2401 caaaatcaaa accgcaagat ttagccaggg ctagtttctc tacccaactg gtattcctgg
     2461 gtagcgcttt ttcataaata cctaacgggt ggcgtcgcat tattttccct cacgagtgat
     2521 caattgttgt tgaaagattt tccggctctt attcatgtca cgctgcaact gccatttttg
     2581 tttgatgaat ttaaatgcca atgcggcgat caatgtgatc ccccataccg ccagattgaa
     2641 atgcaaacta agtcccaaca gactaaggcc ggtagcaata acctgaagca caagtaaggc
     2701 acaaaaaaca tgactgactt tacccgcccc accgaatgga tctgtgcctc ccaatacaat
     2761 tgcaagaacc gtcaagagca aataggaatc gccatagccc atacgcgccg aattaaatcg
     2821 cgcgagcata ataagacctg ctaatacaca caacatgctg gatatagcat atatagcaat
     2881 taatacgcgg tcggtgcgaa taccgctaaa ccatgtggcg ttaatattac tgccacacat
     2941 ataaatcgtt tttcccgtac gggttttgcc gaggaacaga gctaaagcaa cggtggtgac
     3001 gataaaaatc atcatcggta ctgggatccc catgataact tcagacccaa tactgcggac
     3061 tactggtggc ataccactta aagctgcccc cttagtcagg taaataccga taccactaat
     3121 aatggtcatg ctacccaatg tgaccaaaat agggtgagcg cctatatgag aaataattaa
     3181 cccagtaata gctccaatga tgatggcaat aagcatcgcc ccgacaatag ccaggaagag
     3241 ccaaaacaac tgactacctg tagatgcatc tgcgggtaca tattgaatca atacccaggc
     3301 cataaacagg ctggtcagat tcgcagtacc gatgattgcc agattaagcc ccccgctcaa
     3361 catcggtata aacatcgcaa acgtgagcaa cccaagctct ggcaattgaa atgcaatgct
     3421 cagaaaagta ttgtcggtga aaaaacgccc cggcattgcc agactaaata acaccaccac
     3481 aataaaacat attgataata agcctgaacg ggtaccatct agtggaaaaa atatccgctt
     3541 gttcatttcg atcctcccgt tagacgaaac caacatcagt ttctttacgc tttttgtaat
     3601 gggtcacaca aatcgccgag acaataacca ccccaataac gatattgacg aaataactcg
     3661 agacaccaat caaattaagg ccgtttttaa gaataccgat aagaaatacg cccataagcg
     3721 taccaatgac cgtaccacgt ccgcctgata aactggcacc gcccaatacc gctgcagcca
     3781 gcacatccaa ctctccgcca accaatgcat tcggcacaac ttcactcata cggtaagctt
     3841 gcagcattcc accaattgct gcagcggcac caagataacc gtaagcaaac agataaatta
     3901 gcgacacccg aatcccaata cgtcttgctg attcggtatt ccctcccacg gcataaagct
     3961 gacgcccaat atgggttttg ttcattatca cccacgtcag aaatgccatt cccagcataa
     4021 ccactaacgg taatccgatc tggtaatctt ctccgccaaa ttcaaaactc aggaaagaac
     4081 gctgcgtaat ccaccaatcc ggtaaatcgt aaatactgtg accattagtc aaccacatca
     4141 acataccgaa cagtaacgac tgcatactaa ccgtaatgat gatggaaacg acattaaggt
     4201 aataaataag aatagcgttt atcaaaccaa aaatgatgcc aaaaaaaaca gcaaacacca
     4261 ggcagaaaaa tggatttgat agcgtttcat taagcagtaa cgacgccaca acatattgta
     4321 caaccgaagc tactgctgca aaagagatat caattccgcc agtaaccaat acgacaaata
     4381 atccgagggc gaaaataccc gttacggcat aactttccga gagatcgagc aagttctgta
     4441 ttgataaaaa ctcatcgctc atgatggaaa aaaataccag catgagaaca atgacccatc
     4501 ctaaccaagc ttcgactgaa cgaggaaacc gcagccgcat aaagctaacc attaatcacc
     4561 tccgccaact gctgctcggt ataattcgaa ggcaacagct catgaataat ctttccgcgc
     4621 ttcatatgaa aaatacgatc gcagttataa taagcttctg gtgcttcatc agtaattaat
     4681 atcactgaaa tacccacacc tgaaagccga tgaataagct ggtaaatact atcttttgcg
     4741 ccaatatcta cacctaccgt tggcgcatcc agaattaaaa ccttaggtcg tgttaaaatc
     4801 catttagcca ggacaacctt ttgttgattg ccaccagata gcgttgaaag tggattattt
     4861 ggatcggtga ccttaatatc caaatcctgg atccactcct tgactaaggt ttcttttcga
     4921 ttttcatcaa taaaccccga tggcgtcttt aaacgatcca gaatcgagag caccatatta
     4981 tcgctgatag attggggtaa tacggccccc agtgtcaggc gatcttctga aacatatgct
     5041 attcccagtt ttaccgcatg cgtgttgtct ttaattgtaa cgggcttacc ttcaagataa
     5101 atatcaccac tatctgctcg agtaataccg aaaagactca atgctaattc ggttctcccc
     5161 gcaccaagta aaccacacaa tcccagaacc tcgccctggt gtagttttag agatacatct
     5221 tcaaattgcc cttttctgca taattttttt agttcaagaa ctactttacc ttcaacgcta
     5281 tttggtagtt ttttctggtg gactatagtt tttccggtca ttaactcagt aatatcgcct
     5341 ggagaaagtt gattggcagc ccacgttcca attatctctc catcacggat gaccgtaatt
     5401 cgatctgaga tttccttaac ttcatcaagg cggtggctaa caaaaactac ggtaatattt
     5461 ttatcgcgta agtaattcac tgtgcgtagt aattgattaa cttcagtgcg cgtcaatgac
     5521 gccgttggct cgtccatgat gactaaccgg gcatcggcaa tcagagcccg gcaaatagcg
     5581 acctgttggc gttgtgcaat cggcaaatgt tgtactaacg catcaggatc gagattgaat
     5641 tccagctcct gaattatttt ctgtgataca tcccgaagtt gtttttttct gtgcagccct
     5701 aaatatccct tcaggttgta ttcaaaagcg atattttcag ccacagaaag atttgggaat
     5761 aaagaaagat cctgatatat cacctgaata cccagttccc gagctttatc aggagaaaga
     5821 cgagtccagg acttaccatc tataataatt tcacttcccg aatcaggaac ataaacacca
     5881 cacagcgttt tgatcagagt acttttacca cacccattcg caccagcaag acaatgaacc
     5941 tcacctttat ttaaggtaag attgatattt tttagagccc tatggccacc gaaacttttc
     6001 gttagtgacc ttagcgtcac taatggttgc tccgcattct tctctataat atgaggcatc
     6061 ataatattcc tgaatacgac taaaggaatt ccgggtcaaa tttgacccgg tgatttacgg
     6121 taaaattaca agcctaactt cactaaatgt cgtgtatttt caagatcgag cttttctgga
     6181 ttatcactat aaatagtgtt gttattaacc ttgatctcgc ccatatcacc gatttttaca
     6241 ccgtctttaa taggttcacc gttcatcatt gccgtcgcaa cctggacaaa aaccttaccc
     6301 gcaatcatag ggtttgaaat atatccccca tcaatagcgc ctttttcgag tagcttaatt
     6361 ccctgacctg gagtgaaggt tccaaaaacg caaattgcgt catttttctt ccgcttatca
     6421 atagcacgcc ccgcaccaat tggtccttgt gaaccaaatg acataatgcc tttaagatct
     6481 ttatatttag acattaaatc gttcgtcgtg cgcattgaat catccaccga ttcagcaaca
     6541 ccaaaacgat cttcaaccaa cgtcatattt ggataatgtt ctttttgata agcaatcgcg
     6601 gcattggccc agtcatttac caaaggcacg gtcagactgc cgacaaacat ggcgtattta
     6661 ccttcttcgc ccatgcactt cgccatatct ttcatatgat tggcacccat tgtctgggta
     6721 tttaacaact caaaatccca atctgcattt ttttgatctg gtgattcgtg ggtaataact
     6781 ttaataccgg cttcttgtgc acgcttaagt acaggttcaa gaacctttgc atcgttagga
     6841 acaaccccaa taacatccac ttttcgtgca attaaatctt caatcgcccg aacctgttca
     6901 gctggatctg ccgttgtagg gccgacctgc catgcatcaa cccccaatag ttttccttca
     6961 tctttaatgc cttgttccat cacattaaac cagggaatgc caccgacttt tactactacc
     7021 cccatcgtaa aaggtttatt cgctttttgt gatgttacag tctgatcagc ccatgcagca
     7081 gtggaagata atacggtagc aataaggcag ggtaagatta attttttcat cgtaatttcc
     7141 ttaagaaata gttca
//
LOCUS       DARNVM010000210         7153 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.210, whole genome shotgun sequence.
ACCESSION   DARNVM010000210 DARNVM010000000
VERSION     DARNVM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.210"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            352..1389
                     /gene="dusA"
                     /locus_tag="SI621_004310"
     CDS             352..1389
                     /gene="dusA"
                     /locus_tag="SI621_004310"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEI4066947.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1523..1765
                     /gene="pspG"
                     /locus_tag="SI621_004311"
     CDS             1523..1765
                     /gene="pspG"
                     /locus_tag="SI621_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HEI4066948.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1931..2914)
                     /locus_tag="SI621_004312"
     CDS             complement(1931..2914)
                     /locus_tag="SI621_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEI4066949.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2997..4412
                     /gene="dnaB"
                     /locus_tag="SI621_004313"
     CDS             2997..4412
                     /gene="dnaB"
                     /locus_tag="SI621_004313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEI4066950.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4465..5544
                     /gene="alr"
                     /locus_tag="SI621_004314"
     CDS             4465..5544
                     /gene="alr"
                     /locus_tag="SI621_004314"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEI4066951.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGLRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5797..6990
                     /gene="tyrB"
                     /locus_tag="SI621_004315"
     CDS             5797..6990
                     /gene="tyrB"
                     /locus_tag="SI621_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HEI4066952.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GVGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGIYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1673 a         1770 c         1908 g         1802 t
ORIGIN      
        1 cttcgatagt atggaaggca ttatataaaa ggacccaatg cttattgggt tctttttttc
       61 tctatcagtg ctattagcag ggagagatat caccgtagtt taatgtgtga tttttattta
      121 tcgtcgaacc tggattgttt atcattggcc ttaacaaagc taacggctaa taagattatt
      181 tccatcactt cgtgagagct tcatgccttg agaggatctc aattttcttt tgcaatgaga
      241 caggcgcttc ctgttgttat ggtatagtac cccgctattg agcctcctga acagtgatgc
      301 tgaataacat aaccccatga tatatcgata aaataatctc tacatttaaa aatgcacggt
      361 aattctgaaa tgcaaaaaat caaccaaacc agcgcaatgc ctgaaaaaac tgacgttcac
      421 tggagtggtc ggtttagcgt tgcaccaatg ctcgactgga cggacagaca ttgccgctat
      481 ttcttgcgtc tgctttcccg caatacgttg ctgtataccg aaatggtgac tacaggggcg
      541 attattcacg gtaaaggtga ttacctggcg tacagtgaag aagaacatcc ggtagcattg
      601 caactgggcg gtagcgatcc ggcggcgctg gcgcagtgtg caaagctggc agaagcgcgc
      661 ggatatgatg agatcaacct gaatgtcggc tgcccgtctg accgggtgca gaacggcatg
      721 tttggggcgt gtctgatggg taatgcgcag ctggttgccg actgcgtgaa agcgatgcgc
      781 gatgtggtgt cgattccggt gacggtgaaa acgcgtattg gcatcgacga ccaggacagc
      841 tatgaatttc tctgcgattt catcaacacc gtttccggca aaggcgagtg tgagatgttc
      901 atcatccacg cacgtaaagc ctggctttcg gggttaagcc cgaaagaaaa ccgtgaaatc
      961 ccgccgctcg attatccgcg agtgtatcaa ttgaagcgtg actttccgca tctgacaatg
     1021 tcgattaacg gtggtatcaa gtcgctggaa gaggctaaag cgcatttgca acatatggat
     1081 ggcgtgatgg tcgggcgcga agcgtatcag aatccgggta ttctggcggc ggtagaccga
     1141 gagatctttg gttcctcgga taccgatgcc gatccggtgg cggtagtgcg cgccatgtat
     1201 ccgtacattg agcgtgaact cagccagggg acgtatcttg gccatattac ccggcatatg
     1261 ctgggcttgt tccagggtat tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat
     1321 gcccataaag cgggtgcaga cattaatgtg ctggaacacg cgctcaaact ggtggcggat
     1381 aagcgttaac ttttcaccaa aaaatagtca aattcaccac gccctgcgca ccgtcgcggg
     1441 gcgttttgct gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag
     1501 atttcatatt tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat
     1561 gttgatggtc accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc
     1621 cattatgttc ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactcct
     1681 ggcgattgcg gtggtgtggg ttattaaggc gattaaagca ccaaaagtgc cgaaatatca
     1741 gcgttatgac cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat
     1801 ccttagaaca aaataggaat tgataatcaa atctgtcact attgcgcctc taacagattc
     1861 atcgtgctgt accctacata cagccgaact ataaaaagaa agggcttccc aggtggaagc
     1921 cctatttctt ttatggaatc aacaggctgg aaccttgcgt cgcccggctt tccagaatct
     1981 catgcgcacg ctgcgcatcc ttcagcggat atttctgctg ctcggcgaca tcgaccttaa
     2041 tcacaccgct ggcaatcaaa gagaacagtt cattactggc ctcggttaat tcctcccgcg
     2101 tggtgatata gccttgcagg gaagggcgtg tcacatacaa cgagcctttc tgattgagaa
     2161 tgcctaagtt cacgccggta accgcacctg atgagttgcc aaaactcacc attaagccgc
     2221 ggcgttgcag gcaatccagc gaccgttccc aggtgtctct gcccacggaa tcgtacacca
     2281 cgcgcacttt cttgccaccg gtgatctctt ttaaccgctc gaccagattc tcttcgcgat
     2341 agttaataac ctgccatgcg cccgctttta gcgcactctg cgctttttgc gcggttccca
     2401 cagtgccgat aagtttcgcc ccaagggctt ttgcccactg gcaggcaatt aagccaacgc
     2461 cgccagccgc tgcgtggaac aggaactgct catcgggttt aatttcatag gttttgcgca
     2521 gcagataata aaccgttaag cctttcagga aggatgccgc agcttgctca aaagaaattg
     2581 ccgcaggcag aatcgccgct ttatccgcat taatgttatg caccgagctg taagcgccta
     2641 acgccgactg cgcatagact acacgatcgc ctgccttaat atgctttaca ccactgccga
     2701 ctttactcac gatgcctgcc gcttcggtac ctaatccgct gggtagtgat ggcggcgggt
     2761 aaaggccgct gcggatatac gtgtcgataa aattgatgcc gatggcttta ttttcgacct
     2821 ggatttcatt ctccgccgga tcggcaggag tgaactctac ggcttgaagt acttccgggc
     2881 caccgtgctt gtgaaattca attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg
     2941 tctgcttcat atggtaaagt ttcgacccat tctttatctc ggtaactcca ttcactatgg
     3001 caggaaataa acccttcaac aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg
     3061 ccgggctgaa agtgcctccg cactcgatcg aagcggagca gtcggtgttg ggcggtttaa
     3121 tgctggataa cgaacgctgg gacgatgtag ccgagcgtgt ggtggcagac gatttttaca
     3181 cccgcccaca ccgtcatatc tttactgaaa tggcgcgttt acaggaaagc ggtagtccta
     3241 tcgatctgat tacccttgcg gaatcgctgg aacgccaggg gcaactcgat agcgtcggtg
     3301 gtttcgctta tctggcggag ctgtcaaaaa atacgccaag tgcggcgaac atcagtgctt
     3361 atgctgacat cgtgcgtgaa cgtgccgttg ttcgcgagat gatttcggtt gcgaatgaga
     3421 ttgccgaagc cggttttgat ccgcaggggc gcaccagcga agatctgctg gacctggctg
     3481 aatcccgcgt ctttaaaatt gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca
     3541 tcgctgatgt gctcgacgcc acggtggcgc gtattgagca gttgtttcag cagccacacg
     3601 atggcgttac cggggtaaac accggttatg acgatctcaa caaaaaaacc gctggcttgc
     3661 agccgtcgga tttgatcatc gtcgccgcgc gtccgtcgat gggtaaaacc acatttgcga
     3721 tgaacctcgt cgaaaacgcg gcgatgttgc aggataaacc tgtacttatc ttctcgctgg
     3781 agatgccatc agaacagatt atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga
     3841 ctaaaatccg taccgggcag ctcgatgatg aagactgggc acgtatttcc ggcaccatgg
     3901 gtattttgct cgaaaaacgt aatatctata tcgatgattc ctccggcctg acgccaacgg
     3961 aagtgcgttc ccgcgcgcgc cgtattgccc gtgaacacgg cggcatcggg cttatcatga
     4021 tcgactacct gcaactgatg cgcgtaccgg cgctttccga taaccgtacg ctggaaattg
     4081 cagaaatctc ccgctcgctg aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc
     4141 tgtcccagtt gaaccgttct ctggaacaac gtgccgacaa acgtccggtc aactccgacc
     4201 tgcgtgaatc tggctctatc gagcaggatg cggacttgat catgtttatc tatcgtgatg
     4261 aggtgtatca cgaaaacagt gatttaaaag gcatcgcgga aattattatc ggtaaacaac
     4321 gtaacggccc aatcgggacg gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca
     4381 actatgcggg gccgcagtac gacgacgaat aataattatt ttatgaatta ggtaattaaa
     4441 gcaaacactt atcaaggaac acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct
     4501 ctgcgacaca acctgcaacg tcttcgtgaa ctggctcctg ccagtaaaat ggttgcggtg
     4561 gtgaaagcga acgcttatgg tcacggtctt cttgagaccg cgcgaacgct ccccgatgct
     4621 gacgcctttg gcgtagcccg tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc
     4681 aaacctgtac tgttactcga aggctttttt gatgccagag atctaccgac gatttctgcg
     4741 caacattttc ataccgccgt gcataacgaa gaacagctgg ctgcgctgga agaggctagc
     4801 ctggacgagc cggtaaccgt ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta
     4861 aggccggaac aggctgaggc gttttatcat cgcctgaccc agtgcaaaaa cgttcgtcag
     4921 ccggtgaata tcgtcagcca ttttgcgcgc gcggatgaac caaaatgcgg cgcaaccgag
     4981 aaacaactcg ctatctttaa taccttttgc gaaggcaaac caggtctacg ttccattgcc
     5041 gcatcgggtg gcattctgct gtggccacag tcgcattttg actgggtgcg cccgggcatc
     5101 attctttatg gcgtctctcc gctggaagat cgctccaccg gtgccgattt tggctgtcag
     5161 ccagtgatgt cactaacctc cagcctgatt gccgtgcgtg agcataaagc cggagagcct
     5221 gtcggttatg gtggaacctg ggtaagcgaa cgtgataccc gccttggcgt agtcgcgatg
     5281 ggctatggcg atggttatcc gcgcgccgcg ccgtccggta cgccagtgct ggtgaacggt
     5341 cgcgaagtgc cgattgtcgg gcgcgtggcg atggatatga tctgcgtcga cttaggtcca
     5401 caggcgcagg ataaagccgg ggacccggtc attttatggg gcgaaggttt gcccgtagaa
     5461 cgtatcgctg aaatgacgaa agtgagcgct tacgaactta tcacgcgcct gacttcaagg
     5521 gtcgcgatga aatacgtgga ttaatcgttc tgtaatattt gattgtttgt gccggatgcg
     5581 gcgtgaatgc cttatccggc caataaaatc ctaccaattc aataagttga tattctttca
     5641 tgctcttatg aaggccgtgc ctctggcgga tgtacgtttg tcatgagtct cactctgttg
     5701 ctaattgccg ttcgcccctg aacatccact cgatcttcgc cttcttccgg tttattgtgt
     5761 tttaaccacc tgcccgtaaa cctggagaac catcgcgtgt ttcaaaaagt tgacgcctac
     5821 gctggcgacc cgattcttac gcttatggag cgtttcaaag aagaccctcg cagcgacaaa
     5881 gtgaatttaa gtatcggtct gtactacaac gaagacggaa ttattcccca actgaaagcc
     5941 gtggcggatg cggaagcgcg cctgaatgcg cagcctcatg gcgcttcgct ttatttaccg
     6001 atggaagggc ttaatagcta tcgccatgcc attgcgccgc tgctgtttgg tgcggaccat
     6061 ccggtactgc aacaacagcg cgtagcaacc attcaaaccc ttggcggctc aggggcattg
     6121 aaagtgggcg cagatttcct gaaacgctac ttcccggaat caggcgtctg ggtcagcgat
     6181 cctacctggg aaaaccacgt agcaatattc gccggggctg gattcgaagt gagtacttac
     6241 ccctggtatg acgaagcgac taacggcgtg cgctttaatg acctgttggc gacgctgaaa
     6301 acattacctg cccgcagtat tgtgttgctg catccatgtt gccacaaccc aacgggtgcc
     6361 gatctcacta atgatcagtg ggatgcggtg attgaaattc tcaaagcccg cgagcttatt
     6421 ccattcctcg atattgccta tcaaggattt ggtgtcggta tggaagagga tgcctacgcc
     6481 attcgtgcca ttgccagcgc tggattaccc gctctggtga gcaattcgtt ctcgaaaatt
     6541 ttctcccttt acggcgagcg cgttggcgga ctttctgttc tgtgtgaaga tgccgaagcc
     6601 gctggccgcg tactggggca attgaaagca acagttcgcc gcaactactc cagcccgccg
     6661 aattttggtg cgcaggtggt ggctgcggtg ctgaatgacg aggcattgaa agccagctgg
     6721 ctggcggaag tagaagagat gcgtactcgc attctggcga tgcgtcagga actggtgaag
     6781 gtattgagca cagagatacc agaacgcaat ttcgattatc tgcttaatca gcgcggcatg
     6841 ttcagttata ccggtttaag tgccgctcag gttgaccgac tacgtgagga gtttggtatt
     6901 tatctcatcg ccagcggtcg catgtgtgtc gccgggttaa atacggcaaa tgtacaacgt
     6961 gtagcaaagg cgtttgctgc ggtaatgtaa tgcaggaaag caggctggag ttatccagcc
     7021 tgcagtgaaa ttaaactgtc gtcgctttca ctcttgcttt atagatgatt tttttgatgc
     7081 catcgttcta cgtgagaggt aataaaacgt tgttagttct aacttgttaa gtttatccca
     7141 atgttctgga gtg
//
LOCUS       DARNVM010000211         7100 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.211, whole genome shotgun sequence.
ACCESSION   DARNVM010000211 DARNVM010000000
VERSION     DARNVM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.211"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            127..369
                     /locus_tag="SI621_004316"
     CDS             127..369
                     /locus_tag="SI621_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066953.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQRNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVEYQRKLREEW"
     gene            complement(551..1480)
                     /gene="fdhE"
                     /locus_tag="SI621_004317"
     CDS             complement(551..1480)
                     /gene="fdhE"
                     /locus_tag="SI621_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEI4066954.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1477..2112)
                     /gene="fdoI"
                     /locus_tag="SI621_004318"
     CDS             complement(1477..2112)
                     /gene="fdoI"
                     /locus_tag="SI621_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HEI4066955.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2109..3011)
                     /gene="fdoH"
                     /locus_tag="SI621_004319"
     CDS             complement(2109..3011)
                     /gene="fdoH"
                     /locus_tag="SI621_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HEI4066956.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3024..6074)
                     /gene="fdnG"
                     /locus_tag="SI621_004320"
     CDS             complement(3024..6074)
                     /gene="fdnG"
                     /locus_tag="SI621_004320"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5487..5489),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEI4066957.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6268..>7100
                     /gene="fdhD"
                     /locus_tag="SI621_004321"
     CDS             6268..>7100
                     /gene="fdhD"
                     /locus_tag="SI621_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEI4066958.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1574 a         1885 c         1923 g         1718 t
ORIGIN      
        1 ctgttacatc atatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt
       61 tctttaaaga ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgaa
      121 atatttatga actctctggc aggtatagat atgggcagaa ttttactcaa tttatcgaat
      181 gagttgttta agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggagcta
      241 ttacgggaag cggtagatca atacctggta aatcaatcgc aaacagcaag agccagtgct
      301 tttggcatct ggcaagggag tgaggaagat ggtgtcgaat accagcgtaa gctgcgcgag
      361 gaatggtaat ggcaaaaaga tttggctatt tgatatcgat attgttatcg atttgttcag
      421 tgtgaaaatt gaggcaaaac aggcactggc agcatagctt gaggtggtaa cgcggaataa
      481 gagagatagt gtcgtcacac cctatgagct gtaatccggg cgaccagcgc cacccggaaa
      541 acgtcagaga ttactcccct tcacccggga acaggaacgg gttgatggaa ctgcgggcgt
      601 agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct gccacggctt
      661 caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag tcatcgcagc
      721 tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct
      781 gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac aggttgcagt
      841 gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc ggcatagagc
      901 cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg ccggggatca
      961 gattagccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc gctttatcgc
     1021 tgctgacgga cgagaaatca gaggcaaaca gtgcgctggc catatcttcc agctcctgag
     1081 tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt tcaggtttca
     1141 gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc agaacgtgaa
     1201 tatccagcgg gggcttgcct tgtgcgctgg cttctttaat gcgcgtggtc agatccatct
     1261 ccagcggatg gtcgtacagc accacttcct gggcgtgggc gataagcgca gcaaagcgca
     1321 ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg cggcggttgt
     1381 ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta cgtttctcgc
     1441 tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct tttccgttgt
     1501 cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc tggtaaccca
     1561 tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat
     1621 gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga atcggatcac
     1681 cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc tcaccagcag
     1741 cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt tataacgccc
     1801 ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc aaaagatatc
     1861 atcccgattg attaggttgt gatgccagta acggaaaaac atgatgatga acgaggcaaa
     1921 cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc ccatgatttg
     1981 catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa
     2041 gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc
     2101 acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc ttcctcatcc
     2161 gcacggttcg gaccgacacc cacgtagtgg aagatactgg ctgcgaaggt agccgcaaag
     2221 ccaacagctg cgagcggttt ccagatgcct ttccagaatt tcacggtttc gctgatttcc
     2281 gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag cacgtacata
     2341 acgtgtgtac caccaacgcc tgccggatcg tacagacccg cattgtcgta accgcgagtt
     2401 ttcagctccg ccacgcgctc gctcgccagc gttttcatcg actctttcgt accgaagtga
     2461 atcgcgcccg ttgggcaggt cttcacgcag gccggttctt gcccaaccac cacgcggtca
     2521 acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg aatgtcgaac
     2581 ggacagcccg caatgcaata accgcagccg atgcactgct cggactggaa gtcgacgata
     2641 ccgttggcat actgaataat tgctccttcc gccgggcacg ctttcaggca gcctggatcg
     2701 gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt ctgttccact
     2761 tccgagaagc gcatcaccgt ccacgatttg gcacttaaat cattggggtt gtcgtacacc
     2821 ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc cacctgacag
     2881 gctttacagc cgatacaggt ggtgacgtcg atgagtttcg ccacttcttc ctggaagtcc
     2941 cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat atcttgagat
     3001 tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa ggacttaaac
     3061 tctggcgtct gcgtgttcgc atcaccaacg aatggcgtta acgtattggc aataaagcct
     3121 tttttcgcaa caccttcata gccccagtga atcgggatac cgatggtatc gatatctttg
     3181 ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt tatatagcca
     3241 cggttggagg agactttcac tgtatcgccc tgggcaatgc caagtttatt cgccagcgac
     3301 tcaccgattt ccacaaactg ctctggttgc aaaatcgcgt tcaacagcgc gtgtttggtc
     3361 cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa cttatcggct
     3421 ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ccgggttcga gataacgttt
     3481 ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga
     3541 ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg catgataaac
     3601 ggcccaacgc cactgcccgg aggcgctgcg ctgtagtccg gaatatccca gccggtccac
     3661 ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc ctgcggatct
     3721 gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc
     3781 gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt gccctctggc
     3841 gtccagctac cggcgaaaat ccagcagcca caggaggtgg taccgtcatc gcgcagttgg
     3901 gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc cggatcggta
     3961 atatcggcca gcgccttacc gttgctctcc atcgccactt cttccgactt cggctcatgc
     4021 gggatggcgt agttccaggt catgttcagc acctggtccg ggttcgcgcc accctgttcg
     4081 gcatacatct tgcgcaagcg caggaagata ccggagagga tctcgccgtc agtcagcgca
     4141 atcccagggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga gttaacgatt
     4201 gaaccgttct cttccgcgaa gcaggtcgat ggcagacgga acacttcggt ctggatcttc
     4261 gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt ttcagtgttc
     4321 agcgggtcga tagtgacgag gaacttcagt ttcgacagac aactgatcac tttgtttttg
     4381 ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac cttgccttct
     4441 ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg caaccagtca
     4501 aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat catagagacg
     4561 aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg ttttggcgtg
     4621 ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt catgtaacct
     4681 ggcaggctct gcgacagcag ccccaggtcc gtcagcccct gaatattaga gtgaccgcgc
     4741 agggcgttaa cgccgccgcc tgccatcccc atgttgccga gcagcagctg gatcatcgcc
     4801 atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc atacaggaac
     4861 gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt caggaacgcg
     4921 tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga aacgtgctgt
     4981 ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt ggcgaagccg
     5041 ttttcgtcca gttcgtaagt ccaggaggat ttatcgtact tgcgtttttc cgcgtcatag
     5101 ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct ggcgttggta
     5161 taggcttcgg tgtattcgcg gttgaatttt tcattgttca gcaggtacag caatacgcct
     5221 gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc caccgccgcc
     5281 gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat tttggcttcc
     5341 atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat caccacgacg
     5401 aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     5461 gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata
     5521 cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga cgcggaagca
     5581 cacagcatcc cggtggagag ccagcggtta acggtcacgc cttcggcgtt ttgcgcaatg
     5641 tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa
     5701 ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt cagacggctt
     5761 tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc gcggttgacc
     5821 gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt tgctccgtca
     5881 ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt attacgggtt
     5941 tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct gggggcaaaa
     6001 cccagtgccg ccgccgtggt gcctgccata ccgccagcgc agatcttaaa gaactgcctt
     6061 ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt tacattcgct
     6121 tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc aaaggtccag
     6181 aattggctca atttccctcc atccaggaag ggtttgtaac agaataccat aatgttggtg
     6241 tgtgtgttct tatctggtta agagaaagtg aaaaaaacac agcaaaaaga aatcgaaaat
     6301 gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga tttacaacac
     6361 ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa cggcatttcg
     6421 catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg tttttcgctt
     6481 tccgagggga taatcgaaag tccgcgcgat atctttggca tggatgtcgt tccttcctgt
     6541 aatggtcttg aagttcaaat tgagctttcc agccgccgct ttatggggtt gaaggaacgc
     6601 cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gtgtagaaca gcttaacgat
     6661 atcgggaaac cggtgcagcc gctgccgttc acccagacgt ttgatctcaa caaactggat
     6721 gatgcattac gtcatctcaa tgatttccag ccagtggggc aactgactgg ttgtactcac
     6781 gccgctgcat ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc
     6841 catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag ctggcagcaa
     6901 ggtgcggtgc tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgt
     6961 ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacac ttgctgtaga agtggctgaa
     7021 cgctgtaatt tgacgctggt gggcttttgt aaaccgggtc gggcaacggt ttatacccat
     7081 ccgcagcgtt tgagcaatta
//
LOCUS       DARNVM010000212         6951 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.212, whole genome shotgun sequence.
ACCESSION   DARNVM010000212 DARNVM010000000
VERSION     DARNVM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.212"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            555..1544
                     /gene="araF"
                     /locus_tag="SI621_004322"
     CDS             555..1544
                     /gene="araF"
                     /locus_tag="SI621_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HEI4066959.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1614..3128
                     /gene="araG"
                     /locus_tag="SI621_004323"
     CDS             1614..3128
                     /gene="araG"
                     /locus_tag="SI621_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HEI4066960.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3143..4129
                     /gene="araH"
                     /locus_tag="SI621_004324"
     CDS             3143..4129
                     /gene="araH"
                     /locus_tag="SI621_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HEI4066961.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLVIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4296..5096
                     /gene="otsB"
                     /locus_tag="SI621_004325"
     CDS             4296..5096
                     /gene="otsB"
                     /locus_tag="SI621_004325"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEI4066962.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5071..6495
                     /gene="otsA"
                     /locus_tag="SI621_004326"
     CDS             5071..6495
                     /gene="otsA"
                     /locus_tag="SI621_004326"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HEI4066963.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6502..6930)
                     /gene="uspC"
                     /locus_tag="SI621_004327"
     CDS             complement(6502..6930)
                     /gene="uspC"
                     /locus_tag="SI621_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HEI4066964.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1734 a         1624 c         1814 g         1779 t
ORIGIN      
        1 gctttttgta tcttgttgat attaatcggt gaatttgaat tcatgatgaa gcaatgattt
       61 tttaaaacgt tttttcattt ttgcgatatg gctctcggta tacgtgttct gttaattaat
      121 gagcacggtg ataattaatg gcgaaatgaa tgacgcgctt tttccacatc tttgagttgc
      181 ggttattaca ccctttcaaa aactcaacgc caggtaatgt ggcctattga ctggttaaaa
      241 aaaagacatc ccgctgggta ccaaagacaa caaggatttc caggctaatc ttatggatta
      301 atctgctgtg cactagacaa tttgtcagac aaattggctt ttccttatgt cttttcccgc
      361 taaatttatg cacgttctca ctgtaattct gcgatgtgat attgctctcc tatggagaat
      421 taatttctcg ctaaaactat gtcaacacag tcacttatct tttagttaaa aggtaatgct
      481 ttgttttccg attaatttaa cgaatgtcat tcgtttttgc cctacacaaa acgacactaa
      541 agctggagag aaccatgcac aaatttacta aagccctggc agccattggt ctggcagccg
      601 ttatgtcaca atccgctatg gcggagaacc tgaagctcgg tttcctggtg aagcaaccgg
      661 aagagccgtg gttccagacc gaatggaagt ttgccgataa agctgggaag gatttagggt
      721 ttgaagttat taagattgcc gtgccggatg gcgaaaaaac attgaacgcg atcgacagcc
      781 tggctgccag tggcgcaaaa ggtttcgtta tttgtactcc ggacccgaaa ctcggctctg
      841 ccatcgtggc gaaagcgcgt ggctacgata tgaaagtcat tgccgtggat gaccagtttg
      901 ttaacgccaa aggcaagcca atggataccg ttccgctggt gatgatggca gcgaccaaaa
      961 ttggcgaacg tcagggccag gaactgtata aagagatgca gaaacgtggc tgggatgtca
     1021 aagaaagcgc ggtgatggcg attaccgcca acgaactgga tacggctcgt cgccgtacta
     1081 cggggtctat ggatgcgctg aaagctgcag gattcccgga aaaacaaatt tatcaggtac
     1141 ctaccaaatc taacgacatc ccgggggcat ttgacgctgc caactcaatg ctggttcaac
     1201 atccggaagt taaacattgg ctgatcgtcg gtatgaacga cagcaccgta cttggcggcg
     1261 tacgcgcgac ggaaggtcag ggctttaaag cggccgatat catcggtatc ggtattaacg
     1321 gtgtggatgc ggtgagcgaa ctgtctaagg cacaggcaac cggcttctac ggttccctgc
     1381 tgccaagccc ggacgtacat ggctataaat ccagcgaaat gctttacaac tgggtagcaa
     1441 aagacgttga accgccaaaa tttaccgaag ttaccgacgt ggtattgatc acgcgtgaca
     1501 actttaaaga agaactggag aaaaaaggtt taggcggtaa gtaatttgcc agaaaaattc
     1561 ccctctgcat gatgcagagg gggtgtgaac gaccagtgat tcacggagac gttatgcaac
     1621 agtctacccc gtatctctca tttcgcggca tcggtaaaac gtttcccggc gttaaggcgc
     1681 tgacggatat tagttttgat tgctatgccg gtcaggttca tgcgttgatg ggtgaaaatg
     1741 gcgcaggaaa atcaactctc ttaaaaatcc tcagcggcaa ctatgcgcca accacgggtt
     1801 ctgtagtgat taatgggcag gaaatgtcct tttccgacac gaccgcagca cttaacgcgg
     1861 gtgtggcgat tatttaccag gaactgcatc tcgtgccgga aatgaccgtc gcggaaaaca
     1921 tctatctcgg ccagctgccg cataaaggcg gcattgtgaa tcgctcattg ctgaattatg
     1981 aggcgggttt acaacttaaa catcttggta tggatattga cccggacacg ccgctgaaat
     2041 atctctccat tggtcagtgg cagatggttg aaatcgccaa agcgctggcg cgtaacgcca
     2101 aaattatcgc ctttgatgag ccaaccagct ccctctctgc ccgtgaaatc gacaatcttt
     2161 tccgcgttat tcgtgaactg cgaaaagagg ggcgggtgat cttatacgtt tctcaccgta
     2221 tggaagaaat atttgccctc agcgatgcca tcaccgtctt taaagatggg cgttatgtca
     2281 aaacctttac tgatatgcag caggttgacc acgacgccct ggtgcaggcg atggtcgggc
     2341 gcgacatcgg tgatatctac ggctggcaac cgcgtagtta tggcgaggag cgcctgcgtc
     2401 ttgatgctgt gaaagcacca ggcgtgcgta cgccaataag tctggcggtt cgcagtggag
     2461 aaattgtcgg tctgtttggt ctggtcgggg cagggcgtag cgaattaatg aaaggcatgt
     2521 ttggcggtac gcaaatcacc gccggtcagg tttatatcga ccaacagccg atcgatattc
     2581 gtaaaccgag ccacgctatt accgcaggca tgatgctctg cccggaagat cgcaaagcgg
     2641 aaggcattat tcctgtgcac tccgttcgcg acaatatcaa catcagtgcc agacgcaaac
     2701 atgtgctcgg cggttgtgta atcaacaacg gttgggaaga aaacaatgcc gatcaccaca
     2761 ttcgttcgct caacatcaaa acgccgggcg ctgagcaact gattatgaat ctctcaggcg
     2821 gaaatcagca aaaagccatt ctgggccgct ggttatcgga agagatgaag gtcattttgc
     2881 tggatgaacc tacgcgcggc attgatgttg gcgctaagca cgaaatatat aacgtaattt
     2941 atgcgctggc ggcgcagggt gtggcggtgc tgtttgcctc cagcgactta cctgaagtcc
     3001 tcggcgttgc cgaccggatt gtggtgatgc gggaaggtga aatcgccggt gaattgttac
     3061 acgagcaggc agatgagcgt caggcactga gccttgcgat gcctaaagtc agccaggctg
     3121 ttgcctgagt aaggagagta tgatgtcttc tgtttctaca tcggggtctg gcgcacctaa
     3181 gtcgtcattc agcttcgggc gtatctggga tcagtacggc atgctggtgg tgtttgcggt
     3241 gctctttatc gcctgtgcca tttttgtccc aaattttgcc accttcatta atatgaaagg
     3301 gttgggcctg gcaatttcca tgtcggggat ggtggcttgt ggcatgttgt tctgccttgc
     3361 ttccggtgac tttgaccttt ctgtcgcctc cgtaattgcc tgtgcgggtg tcaccacggc
     3421 ggtggttatc aacctgactg aaagcctgtg gattggcgtg gcagcgggtt tgttgctggg
     3481 tgttctctgt ggcctggtca atggctttgt tatcgccaaa ctgaaaataa atgctctgat
     3541 cacgacactg gcaacgatgc agattgttcg aggtctggcg tacatcattt cagacggtaa
     3601 agcggtcggt atcgaagatg aaagcttctt tgcccttggt tacgccaact ggttcggtct
     3661 gcctgcgcca atctggctca ccgtcgcgtg tctggttatc tttggtttgc tgctgaataa
     3721 aaccaccttt gggcgtaaca ccctggcgat tggcgggaac gaagaggctg cgcgtctggc
     3781 gggtgtaccg gttgttcgca ccaaaattat tatctttgtt ctctctggtc tggtatcagc
     3841 gatagccgga attattctgg cttcacgtat gaccagtggg cagccaatga cgtcgattgg
     3901 ttatgagctg attgttatct ccgcctgcgt tttaggtggc gtttctctga aaggtggcat
     3961 cggaaaaatc tcatatgtgg tggcgggtat cttaatttta ggcaccgtgg aaaacgccat
     4021 gaacctgctt aatatttctc ctttcgcgca atacgtggtt cgcggcttaa tcctgctggc
     4081 agcggtgatc ttcgaccgtt acaagcaaaa agcgaaacgc actgtctgat gcttttttcc
     4141 acaacaattt aacgtttttt cccaccacag ccagccgcca caacggttgg ctgttcttcg
     4201 ttacaaatgg cgacccccgt cacactgtct atacttacat gtctgtaaag cgcgttctgc
     4261 gcaacacaat aagaaaagag aaggaggaga accgggtgac agaaccgtta accgaaaccc
     4321 ctgaactatc cgcgaaatat gcctggtttt ttgatctcga tggaacgctg gcggaaatca
     4381 aaccgcatcc cgatcaggtc gtcgtgcctg acaatattct gcaaggactt cagctactgg
     4441 caaccgcaag tgatggtgca ttggcattga tatcagggcg ctcaatggtg gagcttgacg
     4501 cactggcaaa accttatcgc ttcccattag cgggcgtgca tggggcggag cgccgtgaca
     4561 tcaatggtaa aacgcatatc gttcatctgc cggatgcgat tgcgcgtgat attagcgtgc
     4621 aactgcatac ggctatcgcc cagtttcccg gtgcggagct ggagacgaaa gggatggcct
     4681 ttgcgctgca ttatcgtcag gctccgcagc atgaagacgc attaatgaca ttagcgcaac
     4741 gtattactca gatctggcca caaatggcgt tacagcaggg aaagtgtgtt gtcgagatca
     4801 aaccgcgagg caccagtaaa ggtgaggcga ttgctgcctt tatgcaggaa gctcccttta
     4861 tcgggcgaac gcctgtattt ctgggcgatg atttaaccga tgaatctggc ttcgcagtcg
     4921 ttaacaggct gggcggaatg tcagtaaaaa ttggcacagg cgcaactcag gcatcatggc
     4981 gactggcggg cgtaccggat gtctggagct ggcttgaaat gataaccacc gcattacaac
     5041 aaaaaagaga aaataacagg agtgatgact atgagtcgtt tagtcgtagt atctaaccgg
     5101 attgcaccac cagatgagca cgccgccagt gccggtggcc ttgccgttgg cattctgggg
     5161 gcactgaaag ccgctggtgg actgtggttt ggctggagtg gcgaaacagg gaatgaggat
     5221 cagccgctaa aaaaggtgaa aaagggtaac attacgtggg cctcttttaa cctcagcgaa
     5281 caggaccttg acgaatacta caaccaattc tccaatgccg ttctctggcc tgcttttcat
     5341 tatcgcctcg atctggtgca atttcagcgt cctgcctggg acggctatct acgcgtaaat
     5401 gcgttgctgg cagataaatt attgccgctg ttgcaagatg atgacattat ctggatccac
     5461 gattatcacc tgttgccatt tgcgcatgaa ttacgcaaac ggggagtgaa taatcgcatt
     5521 ggtttctttc tgcatattcc tttcccgaca ccggaaatct tcaacgcgct gccgacatat
     5581 gacaccttgc ttgaacagct ttgtgattat gatttgctgg gtttccagac agaaaacgat
     5641 cgtctggcgt tcctggattg cctttctaac ctgacccgcg tcacgacgcg tagcgcaaaa
     5701 agccatacgg cgtggggcaa agcgtttcga acggaagttt acccgatcgg cattgagccg
     5761 aaagaaatag ccaaacaggc tgccgggcca ctgccgccaa aactggcgca actcaaagcg
     5821 gaactgaaaa acgtacaaaa tatcttttct gtcgaacggc tggattattc caaaggtttg
     5881 ccggagcgtt ttctcgccta tgaagcgtta ctggaaaaat atccgcaaca tcacggtaaa
     5941 attcgttata cccagatcgc gccaacgtcg cgtggtgatg tgcaagccta tcaggatatt
     6001 cgtcatcagc tcgaaaatga agccggacga attaatggta aatatgggca attaggctgg
     6061 acgccgcttt attatttgaa tcagcatttt gaccgtaaat tactgatgaa aatattccgc
     6121 tactctgacg tgggcttagt gacgccactg cgtgacggga tgaacctggt agcaaaagag
     6181 tatgttgccg ctcaggaccc agccaacccg ggcgttcttg ttctttcgca atttgcggga
     6241 gcagcaaacg agttaacgtc ggcgttaatt gttaatccct acgatcgtga cgaagttgca
     6301 gctgcgctgg atcgtgcctt gactatgtcg ctggcggaac gtatttcccg tcatgcagag
     6361 atgctggacg ttatcgtgaa aaacgatatt aaccactggc aggagtgctt cattagcgac
     6421 ctaaagcaga tagttccgcg aagcgcggaa agccagcagc gcgataaagt tgctaccttt
     6481 cctaagcttg cgtaagtgcg gttaatctcc cgtaagtgga actaacagca catcgacctc
     6541 acttgaggca atcactcttt tcgccgagca ggatgctcgc gaaaagaaac tgtgattgtg
     6601 attaccgcaa atcaccaaat cgaaatgata tttgcgacaa acttccagaa tatgttcgct
     6661 taactcaccg taggcaataa acgtcttgtc aacgggatac cccgcatcct gaattaactt
     6721 atcaagaaag ctttgcgttt cttcctgcat cacgctgcgt aaatcttcaa gcatcggcgc
     6781 agctaattga ttgtacattt ccgggtcgga agcgagagta attaaactga tgtgtccctt
     6841 tactggcctg gcgatagata ccgcttttgc cagcagttgc tgactttccg gtgtaaccgc
     6901 aacggcgaca agaatattgc tatagctcat aacctgttcc ttattctgtg a
//
LOCUS       DARNVM010000213         6916 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.213, whole genome shotgun sequence.
ACCESSION   DARNVM010000213 DARNVM010000000
VERSION     DARNVM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.213"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(17..2800)
                     /locus_tag="SI621_004328"
     CDS             complement(17..2800)
                     /locus_tag="SI621_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEI4066965.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIIKDKENRV
                     NGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARANNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRKLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLAMRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2857..5229)
                     /locus_tag="SI621_004329"
     CDS             complement(2857..5229)
                     /locus_tag="SI621_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEI4066966.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5267..>6916)
                     /locus_tag="SI621_004330"
     CDS             complement(5267..>6916)
                     /locus_tag="SI621_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEI4066967.1"
                     /translation="AKYLCLLKPFWLRKNNKTSVLLIIIILAMILGVVKIQVWLNDWN
                     NDFFNALSQKETDQLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNR
                     WFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVIL
                     WQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATF
                     RTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKDNWHRLMNRQRWLDYWQNIYSRS
                     LSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLY
                     EFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSPGKWLLLKGYSGA
                     GKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPLPVDDKSLS
                     EVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIAVARLILRRPKWIFLDETTSHLEEQ
                     EAIRLLRLVREKLPSSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1777 a         1611 c         1554 g         1974 t
ORIGIN      
        1 gttgacgggg ctgttattat tttggcaata atactccggt ataggtattc accggatgag
       61 aaagatattg tttaacggca gtgttaacat tctctaccgt catttgtttc aacaattgct
      121 cctgctcagt ccatgctgca ggatcgtcat attgaataag actatttaca atggtgttcg
      181 ctaattgttg aacgctacgc tgttggatat cgaggctgcg ctgaacgttt tgctggtatt
      241 cgttcagttc ttgctcactg atccctttag ccagacgctt aaccatcact tcattcgcta
      301 acgttaacag ttcatcatgt cgttccggtt gacaagtaaa agccagcaaa tgactgatat
      361 ctttggcctg aggatcaacc gagaggcgag aagaaacgct gtatgcccca gaggcctgtt
      421 cacgaatatt aatgcgtaga tcttttgcca gtgcgacgtt aaaagcatcg agcgccatac
      481 gcatcgccag attaaccggt gtccgggaat cataacgctt ccactgtgaa acctgtgcca
      541 caggttcatt ttgttctttt acagtaaccg atgcgttgtc cgtcgcgcga gttaatggtt
      601 tacctgcggc taatggcgaa tcagagtgtt tgattgatcc taagtaacgc gtaattaacg
      661 ccacgagttt gtcttctgcg acattaccga caatgacaaa cgtgatatcc gctggagatg
      721 aaaacaactt gcgatcggca gccagcgcat ctgcggcagt aaactgtgca atctgatttt
      781 cttgcagtaa tttcgtgcgg tcatcagcat agcgcgtctc atacatctgc tgggcgaatt
      841 tctccgctgg acgctggtcg agtgttttca acgcctgaat ttgagcattt tgtagcgatg
      901 cccaaatatt atcgttaatc gtgctgtggg tgattcgctg gttaatcaac tgaaaaccag
      961 gttcagggtt attagcccgc gcgctaacgg agagcaacgt attcatgcca ctgactttac
     1021 tgctcatggt taccgagttt tccgcactcc agcgtttcag gctggaagag gagagttcac
     1081 caacgccgct tccgcttact gctttatttg ccagcgcgat aagtgatttt tgctgcgcag
     1141 ggaaacttaa atcgccttta ttcgatacgg cagtaatttg tagcttttgc tcttcacccg
     1201 cggattttgc cagaataacc ctggcaccat tggaaagtgt taatgatgtc agattctcag
     1261 ccagcgtttc tttgctgcta atttccgcct gtgggtcagc gtctactgtt aacgataaat
     1321 ttctgcctgg gaagatgtaa gccgccagct ttttgttggc gtactctttt tccagcgcca
     1381 ggatagccgc aggagacaat gcttttttgg cagcaacctc attgtttacc atttgctccc
     1441 aaaatgcatc ctggttcttt cttaactgct gccatttttc cgccagactt tgcacggtaa
     1501 tttgctgcca cagacgtttc gaaagttgat atgtctcttc cggcgacaag aacggcgtat
     1561 tatttaatga gctggatgcc aggcgactgg tcagcatacg taaatcacgt tcagcttgct
     1621 gatcaaccgc atttttcagc caggtgaggc gggtagattt gacatcatcg agttcttcag
     1681 cagaaaaacc atgctgatca atagttgcca actctgccat taatgcattc gcagcatcct
     1741 gcatattatc gtctcgtgca tttacacgga aaaacagcga ctgataatcg ggtgcaattt
     1801 taacgctgcg cgcagtgccg ccagaaatag tcttcaactc gcccgactgt atgcgttcct
     1861 gcagacgctg attgaacagc tgaaccaaca tgctccattc agcttgttcg ataaagcttt
     1921 gctcatcgct cacttgcacc attggcaggc gataatagag tgcgatgccg ttcacccggt
     1981 tttctttatc cttaatgata ttaaagcgca ggtggttttc ggcttttgtc ggccagacgc
     2041 gattttcagc tgctttgtta gccggaagct tacttaaatt gtcctttatc agcgccagcg
     2101 cttctttact gtcgatatcg ccgaccacga taaaggtcat attatttggt tgataccagc
     2161 gttgataaaa ttggcgcaat tgtgccggtg tgaccgtagc gacagtatcc atcaggccga
     2221 taggttcacg gtctaaatta cgggtattag ccagcaggaa agggcggcgc gcctgagagg
     2281 tgcgccattt cgcatcctga tgggcacgcc attcctcagt aattacgcca cgttcagcgt
     2341 ctacttcgag tttttcaaag gttgcggcat tactccattc actgaagatt gccatcactt
     2401 gttgcagatt ttgtttctgc gtagtcggca aactcacctg atacaccgtt tcgtcatagc
     2461 tggtataggc attaacatcg cgaccaaaac gcaggcccat tgactcaaat gtttcgatga
     2521 ctttattacc cggccatgtt tttgtgccgt taaacatcat atgttctaca aaatgagcca
     2581 cgccgcgctc attgtcctct tcctgcaatg aaccggtatg aatttgcagc cataaattta
     2641 cctgatcctt tggttgagca tgcggataaa tcatatatcg caagccattg tccagttgcc
     2701 cggtaattaa cttttcatcc tgcggtaagg cggcggcaat cagtcgcccg gggagcaaca
     2761 gagttgccac taacgtcagt aagaaacaga ggtttctcat aattatctcc atgcgaaaac
     2821 cgggcaaatt tacccggtta agtaaaatcc gaactattaa aatttcatgc tgacatcaag
     2881 ccagaaagta cggccactgg cgtaggtcgc cacaccggta ttcgttgtat caaccgcggt
     2941 tttgctatcc agtacattga gaatatcggc actgatcgtc aggttttgtt gtttcagaaa
     3001 ttgtggcgtc caggacaagc gggtgtccca ggtcaggctg ctatccagtt tttcgtcaac
     3061 atactgcttg taatctgaat attcgctgat gtattgcgca ttcgtcttac ccaggataat
     3121 gcgagcttta cgcgcttctt gccaggtcag cgtattcgcc cacaccaacc cgctcggttg
     3181 atgcgtgaaa tccatgttta aggagatctt tagtggatta ttaaaatccg ccactggaac
     3241 gctatcgtaa gagaccagat taccgttata aaccacccgg ttatcaccgg tattgctctc
     3301 ctcataacca ttgtttaacg acaagttgcc cttgctcttg atatagctaa agacaatttg
     3361 cgggttaata tctacctggc tgatatgcag gggttcggcc agttcaaaac tgaggttgaa
     3421 cgaatgcgtt ttggttttgc cgtcgttgtt atattcagta atggtggttt tagtcgcgct
     3481 gtcggtacga ctgcttttac tgatttgatc atgcgcttca cgataaacat agtttgcacg
     3541 cgcaataacg ttcttaccga ttttctgctg caaccccatt gccagttcat cgttataagg
     3601 cgttttcaaa tcctgataac gcgtcagggt tttattgcct gataccgatt ccgtccagct
     3661 attgcggata tcacgtaatc ccatatcaag aatattcccg ccatagtaac ggttataacc
     3721 tgcggtaatc attgaggttt gatcagcaaa aatatcccat tccgtcataa atcgcgggga
     3781 gatattgtgg tttgacagat agttgtcata gtcataccgc acaccgggca ttaacgacac
     3841 attccgccag ctaatgcgat ccgccatata cagcgtgtag ttgtcaattc ccagactgcc
     3901 tttaccttta tggtaaatgg tatggttagt tttctttcct gcagcattaa tcacatagga
     3961 ttcagactgg ttatggcgtt cagtccaagc atcggaatag atgtattccg cgccgaagta
     4021 gggttgatgc gaaacattac ccacggcgaa tttttgccag tccaggcgtg ttttgaaggt
     4081 gtaattatct acagcctggg aaatgtgtcc taatccgcca cgggtgcaac gccctgaaat
     4141 atcaccatat gtacatgaaa gttcggtgta ccagatgtca tgatcgtgac gggtataatc
     4201 actaatatga tcccaaccaa cggtggtacg cagtttggcc catgcgagct gggtatccat
     4261 atcccatgcc agaccatagg atttattacc catttcgcga tcagactccg ggaaggtgct
     4321 ggtattataa tcacggctgg agccggtata ttttaaggtt aaatcgtggg taaagcggtc
     4381 gctggcaaac caggtaaatt tgctcaatgc ggtatcgata acatttttat actgtgcccg
     4441 accggcgaca atgccgtcat tcgaaacata atccgcgcgg gtgatatcag actggcggcg
     4501 cgataaaccg gcagtaacgc caaagttatc agccagttcc tgattaaacg acaaggtata
     4561 aaagttcttt ttaaaatctg gagagaaata agtacttcct gaagaacctt gattaaatgc
     4621 gctcttgtta ttctcatcga tatgcgatgt taaccagtcc gaacgcgtag tgcggtaacc
     4681 cagtttcacg ctgctatcat cagcgttgaa gcgtttgatc tttgcatcaa ttaccccgcc
     4741 attgaagcga ccaaattcaa ccggcacaaa actgtcataa agcgtcacat tgtccagtaa
     4801 gctgacatca agataatacc cctgtgacat cccgctaata ttggttgcac tactggcatc
     4861 ggactcattc gctgggttca ggttattagt tgcactaata ccatcaatca aataggcatt
     4921 ctggtagggc gacgcaccgt gaatagagat tttttccggg cgaatatctc cctggttcaa
     4981 cgaggtactt tgcgttgaat ccatgcgcac cgcagggttt gttctcagca gatcgctgat
     5041 attgccattc ccggtcggta aacgctcgat gctttcactg gtgtaatgcg tgttgccgtt
     5101 tacgggtaca gggacaggag catagaccgt catctcttca aaaacatgca tgtacatgtt
     5161 tttgtcatcg acggcctgcg ccacatcagc gccacataaa atgacgccag gaataagaac
     5221 tcgcttcatt tatttttatc tccgtacagc agggctttta ttgtttttat aaaaccgcgc
     5281 taatgtcaca aatatcatcg gccaggttcc agacaccggg ttgatgtgta accataatga
     5341 cgccgcttga gggtagtttt tcacgcacta aacgcagtaa gcggatagcc tcttgttcct
     5401 caagatgaga ggtagtttcg tcaagaaata tccatttcgg acgtcgtaaa attaatcgtg
     5461 ccacggcgat acgttgtttt tcgccgctgg aaagaatatc tccccagcga tcgtggtcat
     5521 gaatacgcgc agccaatttc cccagaccaa cctgatgcag tacttcgctc aacgatttat
     5581 cgtctacggg caggggaagt gctttacaaa ttatctcttt cagtaagccg gttttgatta
     5641 acggtgtttg tgacacatac caactgtcag caggagacga aatatcacct ttaaaccacg
     5701 gccagcagtg ggataatgtt ttaagcagtg tggtttttcc cgcaccagag taacctttca
     5761 gtaatagcca tttgcctggc gaaacatgaa agtttaggtt ctctaatatg atcttattat
     5821 caggagtacg aatactcgcg tcagccactt gtaccgcatg ttggcaattt ttaggcttat
     5881 tcgtagggcg ctgttcggtg agttgatgga actcatacaa gcgatcgata accgcagcca
     5941 gttcagcaag ttcatcatat ttataaataa accagcttaa attgttcgat accagcataa
     6001 atgcctggcg cgatttcatc agtccgccca gattgatctg tccgctaata aactgcggca
     6061 ataacagaaa gtagggaaga acgctaagcg agcgcgaata aatattctgc cagtaatcaa
     6121 gccaccgctg acgattcatt aaacgatgcc agttatcttt aatggtatga aaattgtcgc
     6181 tcaactcctg gcgttgtaga gactcagcat tgcttagcgc aatcagttca gcctgcttat
     6241 tatgctgcac aagattagtt cgaaatgtcg cttcgctacg ttgtttttcc acattaagcg
     6301 gacgaatacg tttacccacc ttatgagtaa ataaagttcc accgatcaca atgagcacga
     6361 cggtatagac catatacccc tggatattcc attctgttcc accaacagtg aatgagagcg
     6421 tacccgcgct ttgccaaaga ataacggtaa aggtgatcaa catgctaagt gactggatga
     6481 agccaaatga aagactgagc gttttgctga tcagtaagag aatgtcttca gcgatacgtt
     6541 ggtcagggtt atctgtattt ttatgctcac cgtagatctg cgtgaagtag tagtttttat
     6601 ctgcaaacca gcgattaagg tagtaatctg ttaaccattc gcgccagcga atagtcagta
     6661 gtttaattaa ccatgttttg tttacggata ttaatacaaa gatccccagc agggcaggga
     6721 accaaaggac aagctgccaa agctgatcgg tttctttctg gcttagcgca ttgaaaaaat
     6781 cattattcca gtcgtttaac cagacctgaa ttttaaccac gccaaggatc atggcgagga
     6841 taataatgat taacagcact gaagttttat tattcttacg cagccaaaag ggcttcagca
     6901 gacagagata ctttgc
//
LOCUS       DARNVM010000214         6901 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.214, whole genome shotgun sequence.
ACCESSION   DARNVM010000214 DARNVM010000000
VERSION     DARNVM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.214"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..555)
                     /gene="fimB"
                     /locus_tag="SI621_004331"
     CDS             complement(<1..555)
                     /gene="fimB"
                     /locus_tag="SI621_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HEI4066968.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFY"
     gene            2008..2724
                     /gene="nanC"
                     /locus_tag="SI621_004332"
     CDS             2008..2724
                     /gene="nanC"
                     /locus_tag="SI621_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HEI4066969.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2744..3850
                     /gene="nanM"
                     /locus_tag="SI621_004333"
     CDS             2744..3850
                     /gene="nanM"
                     /locus_tag="SI621_004333"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HEI4066970.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGVIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3915..4895
                     /locus_tag="SI621_004334"
     CDS             3915..4895
                     /locus_tag="SI621_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEI4066971.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(4903..5553)
                     /locus_tag="SI621_004335"
     CDS             complement(4903..5553)
                     /locus_tag="SI621_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEI4066972.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQEIYVEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            5690..6562
                     /locus_tag="SI621_004336"
     CDS             5690..6562
                     /locus_tag="SI621_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEI4066973.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYILAEYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHQKEVFRG"
     gene            complement(6673..6894)
                     /locus_tag="SI621_004337"
     CDS             complement(6673..6894)
                     /locus_tag="SI621_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066974.1"
                     /translation="MNGLSEDFKKRTVTLVEQIPEKQKSRYHINEDALITLPSGEIVA
                     ISNQWGIANIELLIEFVRQNGFVVEKAEQ"
BASE COUNT         2054 a         1485 c         1493 g         1869 t
ORIGIN      
        1 gtaaaaacgc cctgcattgc tggcggtata ccagacagta tgacgaatat tgcgatgccc
       61 aagataatcc tggataagtc gcgtatctat tcccatattc gccaaagcaa aaccacacga
      121 atggcgtaac atatgcggat gaatctccag tgacaacccg gcattaccac cagaagtcga
      181 gataatatgg taaaactgtt gccgagaaag cggattcccc ttacgtgata aaaataccca
      241 ctcgctctca gcatgcggat aagaagtacg gatactcaac cagtttttta aagcctgaac
      301 ttctttgttc aatagcgggt gcgttgttga aaagcctttt tttaatcgat ggatatatat
      361 acactttgcc ttaagatcaa tatccgaaat cctcaatcga caaatttcac tcgcccggaa
      421 accatgaata aaacaaagca aagtcagaca ataattacgt gctgcatgag gcccggtatt
      481 tgctgcttta aggagtgatt cgatttcact atgggtcagg aagttccttt ttttgttatc
      541 agccttattc ttcatcattt ttcccttata attacagacg cgcactagct gtgctgggtt
      601 agcaataatc caacatttta atcctgatta tgtttataaa agcgaacgtt tgcttaataa
      661 ctaacgtaag tgaaccagtt cttctgagtg aacttaaatg gagtagcaac agttaattat
      721 aataacgttg ccatacaaac gctatgctct tgcatgctat gtacctttat atatttatca
      781 atcggatacg aatctaataa taaccctcat cattaatgca actaatctta tctatatatc
      841 atgtgatatg ttctgtaaca agtaaaatca acataaaaaa ttcagcgaaa tttaatattc
      901 gttcaactag ccgtctcaca tatgacatta ttagtcaact ccatttgcca caaaatggct
      961 atttcatgga acacagtaat ttcttagtgg ctattatcaa gctaattctt agatccctag
     1021 cgattattcc tggctggttc gtcagcttag gccatgagat tgttacattc ctttttatcc
     1081 tggagatatg ggggaacgca ggcgacgctt tttcatctac atccgtaggt aactatggct
     1141 ttggtcacta cgacttaaac cttcctcaat cacagctatt cgccgggcga tacggcaaac
     1201 aaactaagtg aaacatcatc ctgaaaacac caacatcaac aagcctctcc agatcgacat
     1261 caggagtgac ctgttacaag ccacaaacca agaaacatat ttcatgatga gaattatgct
     1321 cagcctgatg gcgggtaaac ccttattttc accaagctca ctgagttagc ttgcaaagct
     1381 ttctttgttt agattctaaa taaattattt ataaaacaga tagttaaaag aaaatcctta
     1441 gtttttgaag accaccgcaa gtgttcgtct ggcttcacat ggcatctgcc tcttagaaaa
     1501 agatcgacat attttgtgac acgaattgca aatctggttt tgttgtatgg attgcgtgat
     1561 ttttgatctg gtataacagg tataaaggtg caccacgata gtcaatgaga ctgggcatct
     1621 cgtaatctat ggcaaacatc acttcagttc tttcacatcg ggtgatgaaa atgcacttca
     1681 gtctgaaagg aatatgaaaa tgagatcaac agacactcta ttttatgact ctgggtaaaa
     1741 tgaattgagt aagtgatata gcttacgaac attcaaatca attaaacatc agaagagatt
     1801 ttatactcag gtatttaatc tggttctctg tttatttaaa taatgtggaa agagaatttt
     1861 cacaggagac cttatacaaa aaatataaaa taagctaccc gttgccaaag acactataag
     1921 cctggcaaaa aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt
     1981 tctgtatcct acaaatgagt gaaatttatg aaaaaggcta aaatactttc tggcgtatta
     2041 ttactgtgct tttcgtcacc attaatttct caggctgcga cactggacgt acgtggtgga
     2101 tatcgtagcg gaagccatgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat
     2161 ggatggtggg caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa
     2221 aatgccgcac tcaatgatgt tcaggttgaa gttaattacg cgattaaact tgatgatcaa
     2281 tggacggtgc gcccgggaat gttaacgcat tttagcagca acggcacacg ctacggaccc
     2341 tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac
     2401 gactggaaag cttaccgaca acaagactta tccggtgata tgtctcgtga taacgttcat
     2461 cgttgggatg gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg
     2521 acgctataca gcaaacagaa tgattatcgc tatgcaaacc acaagaaatg ggcgacggaa
     2581 aatgcctttg ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac
     2641 taccttgacc gccagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc
     2701 attggtgtgt catttaaact gtagtagaca ggagacagtc acaatgaata aaacaataac
     2761 ggcgcttact atcataatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc
     2821 tgtgccattt aaaagtggta ctggagtaat tgataacgac actgtctaca ttggtttagg
     2881 tagcgcaggt acagcatggt acaagctgga tacacaggcc aaagataaaa aatggacagc
     2941 gttagctgca ttccctggtg gaccgagaga tcaagcaacc tctgcattta ttgatggtaa
     3001 tctgtatgtg tttggcggca ttggcaaaaa cagcgagggc ttgactcagg tatttaatga
     3061 cgtacacaaa tacaacccca aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat
     3121 gggcatggcg ggccatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt
     3181 taaccagaat atcttcaatg gctattttga agatctcaac gaggctggaa aagattcaac
     3241 cgctatagat aaaatcaacg cccactattt tgacaaaaaa gcagaagatt atttcttcaa
     3301 taagtttctg ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatctcc
     3361 ctggtacgga acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa
     3421 tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt gaacttgact tcaccggtga
     3481 taatttaaaa tggaataagc ttgctcccgt cgcatcacca gatggcgtcg ctggcggttt
     3541 tgcggggata agcaatgatt ctcttatatt tgccggaggg gccggattca aaggttcgcg
     3601 ggaaaattac caaaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcac
     3661 tgatattcat ctttggcata acgggaaatg ggataaatct ggtgaattat cgcaaggtcg
     3721 ggcctacgga gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc
     3781 aggcggcaaa gcggtgacgg attcagtttt aatttctgtg aaggataata aagtcacagt
     3841 acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc tatcaatttg tcatttgtat
     3901 tacgcgaaaa ttatatgaac gcaataatat cgcccgatta ttactatgtt cttaccgttg
     3961 ctggtcagtc taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg
     4021 cgcctcatcc cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgt
     4081 catgtcactt taacgacatt attccactga ctcactgccc acacgatgtt caggatatgc
     4141 agggttatca ccatcctctg gcaacgaatc atcaaacaca gtacggcacc gttggccagg
     4201 cactgcatat agcacggaaa ttactgccct ttattcctga taatgcaggg gttctcatcg
     4261 ttccgtgttg ccgtggcgga tcggctttta ccgcgggcag cgaaggaaca tattcagaac
     4321 ggcacggagc cagccatgat gcttgtcgtt ggggatcgga tactccgcta taccaggatt
     4381 tagtcagcag aacgcgagcc gcactggcaa aaaatccgca gaacaaattc ctcggcgtat
     4441 gctggatgca aggcgaattt gacttaatga ccagtgacta cgcgtcacac cctcaacact
     4501 ttaatcatat ggttgaagcc tttcgtaggg atctaaaaca ataccattct cagcttaata
     4561 atattactga cgcaccgtgg ttttgcggcg ataccacctg gtactggaaa gaaaatttcc
     4621 ctcatgcgta tgaagcgatt tatggcaatt atcaaaataa tgttttagcc aatattattt
     4681 tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgat gaagatccgg
     4741 acgatttaag cacgggatat tacggttcag cgtaccggtc accggagaac tggacgacgg
     4801 cactgcgaag cagtcatttc agcgcggcag cccgtcgggg gattatttct gacaggtttg
     4861 tagaagcaat tttgcagttt tggcgcgaaa ggtgagcgtt cattaatttg atttattcgc
     4921 atccatcaac gcttttaact cctccggcgt aaacggtggt ttgcgtcggt gcagcatcgc
     4981 atggcaatta gggcacacag gaagcaaatc attaactgga tccagctggt aatcctcttt
     5041 aatggctgaa agcggcacca ggtgatgcac atgaataaag ccttcagcga tatcaccata
     5101 gatcttcacc agatcaacgc cgcatacgtt gcactggcat ccatgatgtt cgaccgcttt
     5161 atctcgggcc tttttatcgc gttcatagcg gtttactgtc acctttatcg ccgcaccttc
     5221 aacgtatatc tcctgcgacg gcaactcatc cgggaacaga acgtcggcta tgggagccgc
     5281 agcggcttta tcctgatgaa tatgaaatcc ctgctctatg agtagcttga cacatttaga
     5341 ttttattccg ccggtgaaat ccgcaggggt aaattgcgtc cccgtcatca atgtcgctgc
     5401 gatgccagca atcgccttcg aggggtaacg tcggccatga tattcaatct catacaaccg
     5461 cgcaggctgg aactgatgag tttctcccgc atcccataaa tggatagctt cgatgatata
     5521 ccgccgcgga agatgatcag ggagtttatt catggagaat gtccttatac gttctcagta
     5581 caggcagcaa aaaaacaaat gagatcaagg taatatatct tcacttgcag gcttgaacag
     5641 cagaaaattt gcgagcataa tcgcaattat ttcactgcca gggatatcaa tggcttccag
     5701 taaatcgcta cagcaggcaa ttgccaatat aaaaatctgg cacaagggtg aacagcgcgc
     5761 gccgcataag ccattgttat tgctatatat attagcggaa tacctgaatg gacatccgcg
     5821 ccttttcgat tatggctcgg aaatctacga acctctgcac agtttactgg aacgttttgg
     5881 gccacagcgt tcacagtacc gacctgatat gccgttctgg cgattacaag gcgacggatt
     5941 ctggcaatta cacaatgccg agctttgctc aacagcaggg agcagccgac aaccaccagt
     6001 gaaggaactg actgaatacc acgttgcagg cggtttcgat gaacagcact acgccctggt
     6061 aactggcaat aaaaaactca tcaatactct ggcgcaacag atactcgaag cgcacttcac
     6121 cgagagtatt caggaagaaa ttgctgacga gctgggtttt gatctccagc aaatccgtaa
     6181 acagcgcgac ccgttatttc gcaaaaatgt attacgcgct tataactacc agtgcgcgat
     6241 atgtggcttt aatatgcgcc acgacgacac caccgtcgcg ctggaagcgg cgcatataaa
     6301 atggaaacag cacggcggtc cttgtgaaat tcctaatggc ctggcgcttt gcgcaattca
     6361 ccataaagcc tttgataaag gatcgattgg cctggatgaa aatatgcggg tactggtatc
     6421 agatgcagta aacggcggag ggatcgttga gagattattc tgggattttg acgggaaaac
     6481 tattgcgttg ccgcaggtgc gtaaaaatta cccttatgag gtgtttgtag agtggcatca
     6541 gaaagaggtt tttagggggt gaggttgata ataccgtgcc cccataatat tgccatctct
     6601 tgcattgaga tattaaaagc tacaaatgtt ctccccattt ttcaatgtag acatttatag
     6661 caatatcaaa atttattgtt cagctttttc aacaacaaaa ccattttgcc gtacaaactc
     6721 aatcaagagt tcaatattag caatacccca ttgattagaa atagcaacaa tttcaccaga
     6781 aggaagtgta atcaatgcat cttcattgat atgatagcga ctcttttgct tctctggtat
     6841 ctgttcgaca agcgtaactg tacgtttttt aaagtcttca cttaatccat ttattaaatc
     6901 a
//
LOCUS       DARNVM010000215         6764 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.215, whole genome shotgun sequence.
ACCESSION   DARNVM010000215 DARNVM010000000
VERSION     DARNVM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.215"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(463..1803)
                     /gene="fadL"
                     /locus_tag="SI621_004338"
     CDS             complement(463..1803)
                     /gene="fadL"
                     /locus_tag="SI621_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HEI4066975.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPMLSQQLIARGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGPAAYSFESEGKAWLFGTNFNYAF"
     gene            2175..2459
                     /locus_tag="SI621_004339"
     CDS             2175..2459
                     /locus_tag="SI621_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HEI4066976.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2640..3950
                     /gene="fadI"
                     /locus_tag="SI621_004340"
     CDS             2640..3950
                     /gene="fadI"
                     /locus_tag="SI621_004340"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HEI4066977.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            3950..6094
                     /gene="fadJ"
                     /locus_tag="SI621_004341"
     CDS             3950..6094
                     /gene="fadJ"
                     /locus_tag="SI621_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HEI4066978.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            6297..>6764
                     /gene="sixA"
                     /locus_tag="SI621_004342"
     CDS             6297..>6764
                     /gene="sixA"
                     /locus_tag="SI621_004342"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEI4066979.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLK"
BASE COUNT         1677 a         1601 c         1796 g         1690 t
ORIGIN      
        1 actcttgtac taatccatta acttcatcat tgttattttc attgatggat tctgggtaag
       61 ctgcgctggt attgttgatt gaaatagatg gtagcatgat tttattccat gttgattgat
      121 ttgttacaaa tatatattac ctaataaaat agactatcaa aaaacgctca tttatttaga
      181 gcctatcttt aatttgagtg tatgagcgca caatagccag ggaggttgag tatcctggaa
      241 atgcagaaaa gtaaactctt tgagaaggac gagcagaacg cctgaaaata tgcgtttccc
      301 ttcaggtttg tctgtggata ccacttactg atttgaaagg gcaagctgcc atcaggctgt
      361 ttgcaaaact caggaggcaa gcaatgagga aactactttc ggtgaagtgg gggagttaaa
      421 gtcacctgca tagcaggtga ctttatccag gcgaacgcgt tatcagaacg cgtagttaaa
      481 gttagtaccg aacagccagg ctttaccttc agactcgaag gaataagctg ccggtccttc
      541 accttcagta aacttcactt tctgaccatg catataagat gcgccaacgt caatggatgc
      601 atcttcatta aatgcgtagg ttaacccagc acttaaccac aagcgatcct ggtcaggaat
      661 ggaaatagaa cgtttatctg ccggaactgg gctatcgtca taggcgatac cggtacggaa
      721 ggtccagttt ttatcgtaat agtaggttgt gcccaacgcg atacgatatg cgtcacggaa
      781 tccttcatct ttatagaaca gagtctggcc actatcgcct ttcgctttca actcctggaa
      841 ctggctccaa ctggtatagg ccatgctgta gtgaattgcc cactgcggcg caacacggtt
      901 ataaccagaa atctcccaca tctcaggcag gttgagcgtc agggagccat tttgtgttgc
      961 acccatcgtg ccatacggta agcccatgct acccaaaata ttgttcagat ttgggttaat
     1021 ggaacttttg taatcccctt cgaagtcgat tttaacttct gaacgatatg tcaggcccca
     1081 acggttatct ttatccagtt cataaagaat acccgcgttc cagccgaagc cccattcgtc
     1141 acctttcaga tgggcaattt gagagtccgg agtattaata ccattggtta atgaagcggg
     1201 tacaccccgg gcaatcaact gctgagacaa cataggcccc agcgaaccgg cgtaacgctc
     1261 aatttttgct ttggcgtaaa ccgcatcaaa accaagacca aaactccagc tattgttaag
     1321 gcgataagcc ccgctaaggt taaggttgat tgtctgcaga tcggttttac cgccatagat
     1381 accagcagcg tagttgtcgg taaattccgt agccaggccg tagttggaag ttattgacgc
     1441 tccccaacca aactgatcgt tgataggcgc gacaaagtgc aggttaggta cccacgctgt
     1501 cggtgcgata tttttagcat ttgcactctg accggttaaa tcagaatgtc cggtaatatc
     1561 tacatccgga tcaatataaa ccgcacccgc agaaaatgtc gggcggtcaa acatggtaat
     1621 caatgcgggg ttacggctaa cgttacctgc atcatcggca attgcacctt cccctgaata
     1681 agcccggccc aggccagagg aagaaaattc gtttaactga aagcctgccg accaggcctg
     1741 ggtggagata agtgccactg cgactgcgag agcagacttt gtaaacaggg ttttctggct
     1801 catgaccata acctcaatga tttattttta tacaaactat gttacgtgct gtaacaggag
     1861 cgcgaagtgt agggtctgag gtacatctta gaaatcagac cagtggcgag agtataggtc
     1921 ggaccagctg gaatgttgca agtatgtttc tatctttttt caaatatgat ctaagaaacg
     1981 cgatccagct ggcaaaattt ggaggtggcg cactcatgga ttcgggtgaa ttttgtttta
     2041 gatcattttt aagtgtgatt tcggtcactt atccgatttt gcagaattaa caactggagc
     2101 agcactttcc ggaagcgtaa aatatccaca acgtttatct ggcaccttgg gtgcacatac
     2161 agaggaaatc aacgatgagt aaatgtagtg ctgatgaaac cccggtttgc tgctgtatgg
     2221 atgttggcac cattatggac aactccgact gcaccgcgtc ttatagccgc gtgtttgcaa
     2281 accgcgcaga agcagaacaa acgctggcgg cgctgactga aaaagcccgt agcgtggaat
     2341 ccgaaccgtg caaaatcacc ccgactttca ctgaagagtc tgacggtgtg cgcctggata
     2401 tcgatttcac ttttgcctgc gaagctgaaa tgctgatctt ccagctgggt ctgcgttaat
     2461 tcctctcggg tacgtacgcc cctgtcagtt gttggcaggg gcgttttatt ttctcttcta
     2521 cttttctctc tgtgtcatag ctcccacttt cccttttctc cacttggcta aatgtaaaaa
     2581 aatggttaag actatgatca ggtcagacca ctttatttat tattttacag gggagtgtta
     2641 tgggtcaggt tttaccgctg gttacccgcc agggcgatcg catcgccatt gttagcggtt
     2701 tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg gttgatttag
     2761 ggaagatggt ggtaggcgaa ttgctggcac gcagcgagat ccccgccgaa gtgattgaac
     2821 aactggtctt tggtcaggtc gtacaaatgc cggaagcccc caacattgcg cgtgaaattg
     2881 ttctcggcac cggaatgaat gtgcataccg atgcttacag cgtcagccgc gcttgcgcta
     2941 ccagtttcca ggcgattgca aacgtcgcag aaagcctgat ggcgggaact attcgcgcgg
     3001 ggattgccgg tggggccgat tcctcttccg tattgccaat tggcgtcagt aaaaaactgg
     3061 cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg aaactcttct
     3121 ctcgcctgcg tttgcgcgac ttaatgcccg taccacctgc ggtagcagaa tattctaccg
     3181 gcttgcggat gggcgacacc gcagagcaaa tggcgaaaac ctacggcatc acccgagaac
     3241 agcaagatgc attagcgcac cgttcgcatc agcgagccgc tcaggcgtgg tcagacggta
     3301 aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg cttgtcgaag
     3361 acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc ccggcgtttg
     3421 atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag
     3481 cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg ctggggtatc
     3541 tgcgcagcta cgcatttact gcgatagatg tctggcagga catgttgctc ggtccggcct
     3601 ggtcaacgcc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg acattgatcg
     3661 atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg ggtagcgaac
     3721 gttttgctcg tgatgtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat
     3781 ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc ggcgcgcgga
     3841 tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt ttagtgaccg
     3901 cctgtgctgc gggtgggctt ggcgcggcaa tggttgtgga ggcggaataa tggaaatggc
     3961 atcagcgttt acccttaatg tccgtctgga caacattgct gttatcacca tcgacgtacc
     4021 gggtgagaaa atgaataccc tgaaggcgga gtttgcctcg caggtgcgcg ccattattaa
     4081 gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga aaccagacaa
     4141 cttcattgct ggcgcagaca tcaacatgat cgccaactgc aaaacggcgc aagaagcgga
     4201 agcactggcg cggcagggtc aacagttaat ggcggagatt catgctttgc ccgttccggt
     4261 tatcgcggct attcatggcg cttgcctggg cggtgggctg gagttggcgc tggcgtgcca
     4321 cggtcgcatg tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg
     4381 attattaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagcacagc
     4441 attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct
     4501 ggtggatgac gtcgttccgc aatccatttt actggaagcc gctgttgagc tggcaaagca
     4561 ggatcgtcca tcttcacgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg
     4621 tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc agggtaacta
     4681 tccggcgaca gaacgcatcc tggaggttgt cgaaacggga ttagcgcaag gcaccagtag
     4741 cggctatgac gctgaagccc gtgcttttgg cgaactggcg atgacgccgc aatcgcaggc
     4801 gctgcgtagt atcttttttg ccagtacgga agtgaagaaa gatcctggca gtgatgcgcc
     4861 gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc
     4921 ttacgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatta acccgcaggg
     4981 cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca
     5041 tctcaaagcc agcgaacgtg acagacagct ggcattaatc tccggaacga cggactatcg
     5101 cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg aattaaaaca
     5161 acaaatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg cttcgaatac
     5221 gtcatcttta ccgattggtg atatcgccgc tcacgctacg cgacctgagc aagttattgg
     5281 cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc ctcatgcggg
     5341 gacatcggcg caaaccatcg ctaccacagt aaaactggcg aaaaaacagg gtaaaacgcc
     5401 cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc attttagcgc cttacattaa
     5461 tgaagctatc cgcatgttga ccgaaggtga acgggtagag cacattgatg ccgcgctggt
     5521 gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa tcgacaccgg
     5581 gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa
     5641 tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta
     5701 tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca tttacccgct
     5761 gattggcaca caagggcagg ggcgactctc cgcaccgcag gtagctgaac ggtgtgtgat
     5821 gttgatgctg aatgaagcag tacgttgtct ggacgagcag gttatccgta gcgtgcgtga
     5881 cggagatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg gtccgttccg
     5941 ctacatcgat tctctcggtg cgggtgaagt ggttgcaata atgcaacgac tggccacgca
     6001 gtatggttcc cgttttaccc cttgcgagcg tttagtcgag atgagcaagc gtggggaaag
     6061 tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata tgaatccgcg
     6121 ctgaatggcg gagtattgct caaaatgtaa acgcatattg actatactta cgccattgag
     6181 gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt
     6241 tctctggttt cagatgaaag aaaacgggcg aatctggtta acaaaagcgg tgcaatatgc
     6301 aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat tcggttcgtc
     6361 ctctgactac taacggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa
     6421 aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag
     6481 aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct
     6541 gcggcgatgt tggtctggtc agtgcctatt tacaggcgtt gaccaatgaa ggtgttgctt
     6601 cggtactggt gatctcccac ttacccttag tcgggtatct ggttgctgag ttatgtccgg
     6661 gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg
     6721 gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaag
//
LOCUS       DARNVM010000216         6755 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.216, whole genome shotgun sequence.
ACCESSION   DARNVM010000216 DARNVM010000000
VERSION     DARNVM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.216"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(23..247)
                     /locus_tag="SI621_004343"
     CDS             complement(23..247)
                     /locus_tag="SI621_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEI4066980.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(387..2042)
                     /gene="opgD"
                     /locus_tag="SI621_004344"
                     /gene_synonym="mdoD"
     CDS             complement(387..2042)
                     /gene="opgD"
                     /locus_tag="SI621_004344"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HEI4066981.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(2267..3610)
                     /locus_tag="SI621_004345"
     CDS             complement(2267..3610)
                     /locus_tag="SI621_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEI4066982.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHSGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            3827..4750
                     /locus_tag="SI621_004346"
     CDS             3827..4750
                     /locus_tag="SI621_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4066983.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(4789..6429)
                     /gene="trg"
                     /locus_tag="SI621_004347"
     CDS             complement(4789..6429)
                     /gene="trg"
                     /locus_tag="SI621_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HEI4066984.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSARANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGEQVSFATV"
BASE COUNT         1683 a         1759 c         1583 g         1730 t
ORIGIN      
        1 ctggctggcg gcttaggaaa gtttacacct ctttgacgct ctcctgctgc aggattttga
       61 gcatctcatc ttttaactgt agcttttgtt ttttcatgcg gaccacttcc gcattgtacc
      121 ctcgaccgtc ggaaccttcc tttctggcaa tttcatgatc aagtttattg tgtttatcaa
      181 acaaggacat aaagcgagga ttttcgtttt tcagacggga tattaaatct cgatattctg
      241 gaaacatacg cactccctgt tagtggctga caaggtgtat ctgaaataca caccatcaga
      301 ttactcattt aacttaggga aaaatgcgag cgggatcgtg tttaaccaga cggcacctcc
      361 cgaagaaggt gccgaagaaa aaacgattaa ctcatcacgc ggtcgtcaac atactgacgt
      421 ttatccggcg ctggcgggaa atactgatac agccatgttt cactgatagc gtcgccctga
      481 caacgcagat acatccgcat atcgaccgga tcagtggagt ccgaagtcgg ataccagtca
      541 aactgaatac gataaccatc aatgggttca atatagagaa tttcgatttg cttcgcttcc
      601 ccactggaaa gcgtaatcac cggctcaatg ccttttggcg cggcagcttt cagatcacca
      661 ccaacgaaat cgacggcaaa acgacgcgcc catttttcgg gatagtgttc acctggcgcc
      721 caaccttccg ggaaaccgcc catgccggta cgcgtcgcca taacgcgcgc taatgggcaa
      781 tgaacaggcg gttgcgcact ccagtacaga cgatactgga atgcaaactc atcacccgct
      841 tttacagctt tttctggctg ccagaagcag acaatgttat ccagcgtttc acccgtagtt
      901 gggatttcca tcaggccgat ggtgccctta ccccacttgt tacgcggctc cacccacaga
      961 cttgggcgtt tgttatacca gcccataatg tcctgataat gggagaaatc acgatccagt
     1021 tgcaataaac caaacccttt cgggttgttg tcggtgtaag cattgaactg caatttttgc
     1081 ggattattca gcggacgaca aatccactcg ccgttgcccc gccacatgga caaacgatca
     1141 gagtcatgga tttgcggatg aatggtgtcg cacatccgac gttcattagt accgcagctg
     1201 aacatgctgg tcatcggcgc aatgcccagc tgtttaatgt ctttgcgcgc atacaggtga
     1261 ttttccacat ccataatcac ctgacttttc tcacaatgga tagtgaactt ataggcacca
     1321 gtaatgctgg cgctatcgag caacgcataa acggtaaagg tggttgcccc cggttttacc
     1381 gtatcaaacc agaaggcggt aaagtcgggg aactcttctt tactgtcggt gtaagtgtcg
     1441 atcgccaggc cgcgagccga tagaccgtat tgatatgtgt cgtcaacggc gcggaaataa
     1501 ctcgcaccga ggaatgatac tacatcacgg cgcgctagtt cgggggcttt aaacacgcga
     1561 aaaccggcaa aaccgagatc gctttgccct tctaattgtt tggtatcaac acccgcatcg
     1621 ttgtatttga acaactccgg gcgaaagtga atttcacgcg ccagatgtgt tgcgggatca
     1681 acagaaaaca tacgaacgcg gcgacggaat cccattccca tatggaagaa ctgagcgtcc
     1741 agttgacggt tctcaacgtt atgccagagc gatttttcgg cgtcgtattg aatactgtta
     1801 taagcctgcg gcgtcattgt cgccagagtg tcaggtaacg gacgcggagc accacgccac
     1861 gcggtttgcg ctaagtcgtg cgccattgac tgtagaatgg agaagtcaaa acgttgggtt
     1921 tgcccgtcgg caatatcaga atctgccgcg aatgccgcct gagaaaaaag agaagcaatg
     1981 ccgctggtac cgcacacggc ggccatagcc attgaacctt taataaatcg tctacgatcc
     2041 atacctgaaa gtgagtcctt ctggtgttgt gaatggtccc gcgctggtcg ttttcagcgg
     2101 caagaaagtg cttatttatg accgctcact ctagacaata tttattaata atccaattgt
     2161 tgaactgaga gaatatgcat aaatcggcaa tttttttatg tcgatgggat gagcctgatt
     2221 cgtttgtaaa atcaggcaaa tttttgcaaa ggcggatggt cagagtttaa agcaaaccac
     2281 agcgacgttt actgcgttct tccgcttcct gataaagctg gaactcatcc aaaaccgggc
     2341 agcccaacgc ctgctcaaat gcttcgcgac tggcattacc gtgactgcat gcctgcgttt
     2401 cattacccag atttgactgg aagatccccg ccgcgctaac aggcaagaaa tcttcatagg
     2461 taatgggttg cgccactacc caaccacgtt caattaaggg ctgtggatcg tctccggaat
     2521 gaatcgcctg acgatgcgcc tcacctgaag gcgtcagacg gtaccggaac catgccaacc
     2581 cttgctgacg cattaaaaac tcactgtcag gaaaagtgcg gaaggtttcc tgtaaatgca
     2641 tttggtgagt gagattatcc tgcccggttc cagcgttacg cagaagatca tcatacagtt
     2701 gtcgcccttt cggcgttaat gccacaccac gctgctctat ttcaccaaag cgcgcggtat
     2761 gcgtaccctg tttctgccct gcaaacaaca ccgtctcttc cagtgcttta aagctggtct
     2821 ggcgtagtaa aataggtacc tcgcggcgcg gcggcccttc gatcagaatt ttgggttcaa
     2881 tcccgcattc aggcatcatc gactgcaccc ggtcaatatc cagcgtacgt ggcgtcaggt
     2941 ggttgatatg gcatccagga aaacagacca catcagcaat caaccgatgt tcgttgtgca
     3001 aggcgcgata ggtttcttca tctaccgttg ctgactggtg ccaacgaaac gtttccaggg
     3061 cttcctgcac aaactcctgt gcctgtgttt cgttaaaacc gccccgctgc tcatattcct
     3121 ctaacagttg tcgacaacgt ggggtgaaga tatcgcgctg acgcagaatc tccgccgctt
     3181 tctggcgcaa aatttcgttc tcgataagct caaggcggag taatgaagta aaaacgcgga
     3241 agggattacg cgccagagaa gcatcatcaa tgggccgaaa tgctgtggaa tgtaccggca
     3301 cccccgcctg cgagagatcg taatagctca ccgggtacat ccccataatg gcaaacatcc
     3361 gccgaagagt agcaagctct tgtgcagtcc caacacgaat cgccccatga cgttcaacat
     3421 tcagtcgcgc cagctcgtct gcatttacca ttttttcgtg cagttgagga ttgttttcca
     3481 gcacagccag attcacatca gctaccagtt ccagcagcgt gccgtactgc ggaacttctt
     3541 gctggtacat ggctgacatt gcctgcgaaa actgttcccg aatctcatcc gccgtgatgc
     3601 tgttcgccat agtgtctgcc tccagtggtc agtaatctgg agtgtaggta accgcattca
     3661 ctccggcggg aagaatttac aaactgtgat ctcgccgcga aaacatcaat attatccatt
     3721 ttgctgtaac ataattgctt taattgttaa taatattttg caatcaagtt atcataatca
     3781 aacaacttca cttgtcagcg acaccgcttc gtttttaaca tcgcttatgg aaaaaaatag
     3841 tctgtttagt cagcgcatcc gtttgcgcca ccttcataca ttcgtagctg tcgcacaaca
     3901 aggaactttg gggcgcgcgg ctgaaaccct taatttgagt caacctgcgc tctctaaaac
     3961 attgaatgaa ctggagcaac tgacgggcgc tcgcttgttt gagcgtggtc gtcagggggc
     4021 gcaacttacc ttacccggcg aacaattttt aacgcatgca gtcagagttc ttgacgccat
     4081 caacactgcc ggacagtcgc ttcatcgtaa agaaggtctt aataatgatg tcgtcagggt
     4141 tggtgcacta cctactgcgg cactggggat attaccttcg gttataggtc agtttcatca
     4201 gcaacaaaaa gaaacgacct tgcaagttgc gacaatgagt aaccctatga ttcttgcggg
     4261 tttaaaaacc ggggaaatcg atatcggcat tggtcggatg tcagatcctg aactgatgac
     4321 cggacttaat tacgaactgc tgtttcttga atcgctgaag ctggttgtcc gccctaatca
     4381 tccgctactt caggagaacg taacgctaag ccgggtgctg gaatggccgg tcgttgtatc
     4441 accagaaggc actgcgccac gccagcattc agatgcatta gtgcagagcc agggatgtaa
     4501 aattccttcg ggttgtatcg aaacgctgtc tgcttcgcta tctcgtcaac ttacggttga
     4561 atacgattac gtgtggtttg tcccttctgg cgctgtaaaa gacgacctgc gtcatgccac
     4621 actggtggcc ctgcctgttc cgggacatgg tgcaggcgaa ccgattggaa tactgacccg
     4681 cgtagatgca acgttctctt ctggttgcca gttgatgatt aacgctattc gaaaatcaat
     4741 gccgttctga aaggtgaagg gatctgtcga tcccttcctt gaacattttc acaccgtagc
     4801 gaaactaact tgttcacccg ctccgcgagg ttctgccaac acagaatgtt tgttcagacg
     4861 gaatacgtcc accgcctcag ttaatcgtgc cgcctgttct tcaagggaca ccgccgccgc
     4921 tgaggcctct tctaccagag aagcattctg ttgcgtcacc ttatccattt cagaaatcgc
     4981 ctggctaacc tgagttatgc ctctactttg ttcatccgag gcggcggcga tttcctgcat
     5041 gatatgtgtg acactcgcga cggcatcaac aatagtgctc atggttttcc ctgccgttgc
     5101 cacctcatcc gaccccaggt caattaacct gactgattca ctgatcaagc cttcaatctc
     5161 tttcgccgcc tgagcgctgc ggcttgcgag tgtccgtact tcgctggcga caacggcaaa
     5221 tccacgccct tgctcacccg ctcgcgcggc ttcaacggca gcattcagtg ccagaatatt
     5281 cgtctggaaa gcaatactgt tgatgacggc ggtgatctca gaaattttct tcgaactcgt
     5341 ggagatagcg cccatcgttt ttactacacc ggaaaccgtc tgcccgccat cgctggcttt
     5401 aatagaagcc tcttgcgcca gtttgctggc atgatgcgcg ttatccgcat tctgtttcac
     5461 cgtcgcagta agttgctcca tgctggcggc agtttgttcg atagccgccg cttgttcttc
     5521 ggtgcgagat gacaggtccg cattgccagc tgaaatttcg ctggtgccac gataaatctc
     5581 ttccgcaccc tgtcgaacag tccctactgt catccccagt gaatgctgca tttgctgtaa
     5641 atgacgactt aagcgaccga tttcattacg acccgccggt tcatcattca tcgtcagatc
     5701 gccactggcg attttttcaa tccgttgtgc ggcatgttgc agtggacgaa tgacgatccg
     5761 acgtagcacc ataaatgtta tcagcgtcat gaccagggca agcacaaacg cgccaatcat
     5821 gaacatccca cccagacggg tgcgctgatg ggccagttcc gccagttggt tggctctggc
     5881 gctacgtatc ttaacggctt tgctcaaaat atcggtataa gcattatcca gcggtcggat
     5941 ctgctcactt tcatgattga taatcgcttc aaacatgcca tttttggcat atttcaacat
     6001 cggttgcata cccgtgatat aagcctggaa gcgttgattt aattcagtgt cgagggcttc
     6061 atcagcaggt gttttcaccg accgattctg ataagcacga taaccttgct gcgactgttt
     6121 aatctccgat tcggcttgcg caatatttcg cttcattgct tccatttcag caatacgact
     6181 cgcagcacca gcctgaatca tattgatccg ggctgaacgc aaaaaatctg aactgttcgc
     6241 taacccggtc cgaatctcaa tctccgcagt aacatcacgc tgatcgcgat cggcctgcca
     6301 gaggaaatag ccagccagag ctgaacttaa tgcgaataga actaagatac cgccaagaat
     6361 gcaggcaaat aacggaacca gcctgatgtg atgcaaaaaa cctaatcgct gtgagggagt
     6421 tgtattcatt accgttgtct ctcgtccagg tttactcctg atagaaagtc atcggcactt
     6481 gacgataatt acttatgacc aaaaacctct ttgcgtcaca tttttacaac aattattgca
     6541 gagaaaatta atgctattgg aatgaagaga gtaacgaatg gctaaactgg ctaacccgaa
     6601 tgacgaaaaa ttctggaaaa cagcacgcaa tttcgtcagg tgattatttt cgtccctaag
     6661 gaaaccacat aggatgctca cgggtgcctg agactttctg ttttcaggct aaccgggaat
     6721 cagaaagacg aaggccccga ccgatatttc tatca
//
LOCUS       DARNVM010000217         6506 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.217, whole genome shotgun sequence.
ACCESSION   DARNVM010000217 DARNVM010000000
VERSION     DARNVM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.217"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1731
                     /gene="espL4"
                     /locus_tag="SI621_004348"
     CDS             <1..1731
                     /gene="espL4"
                     /locus_tag="SI621_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HEI4066985.1"
                     /translation="IVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQ
                     TLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKN
                     TTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQ
                     SVAIIPALPKEQLLMLKGSVDEIPPPLSPATMNLLMAIGQNHQLTQLMTQLQKMPELH
                     RTEMLTAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDK
                     NGFSGLFLAISRKDKNVVTSILNVLPKLAATHHLDNEQVYKFLSAKNRTSSHVLYHVM
                     ANGDADMLKIVLDALPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKV
                     ILEALPCLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVIKTIFNALPTLFN
                     TFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIM
                     DFWQHKAPQKYSAFELAFELDHRVIAELILNTINKMAESFGFTDNPRYIAEKNYMEAL
                     LKKASPHTVR"
     gene            complement(1700..3436)
                     /gene="aceK"
                     /locus_tag="SI621_004349"
     CDS             complement(1700..3436)
                     /gene="aceK"
                     /locus_tag="SI621_004349"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEI4066986.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKENYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTITRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLIYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLAKRHISPALMEL
                     LLQEAPEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCAAPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3485..4789)
                     /gene="aceA"
                     /locus_tag="SI621_004350"
     CDS             complement(3485..4789)
                     /gene="aceA"
                     /locus_tag="SI621_004350"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEI4066987.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPCKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4819..6420)
                     /gene="aceB"
                     /locus_tag="SI621_004351"
     CDS             complement(4819..6420)
                     /gene="aceB"
                     /locus_tag="SI621_004351"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEI4066988.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWSKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADHLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSRGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1623 a         1640 c         1575 g         1668 t
ORIGIN      
        1 atagtatgcc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
       61 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      121 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      181 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      241 agtgttatgg cagccatatt attggtggat aaccatgctt tgacggtccg gctaagaata
      301 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc aaatgtaact
      361 aacgataaaa taagaatcat gagcgaaagt aaagaggata ttaaacacta ttcactgatg
      421 gactttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
      481 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgctaaa aggatctgtg
      541 gatgaaatac cccctccatt atcacctgca acgatgaatt tgctaatggc aattggtcag
      601 aatcatcaac ttacgcaact gatgactcaa ctccagaaaa tgccagaact acatagaaca
      661 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
      721 ggtaatgcgg atatcgttga gactattttc aattcattgt cagagccagg atatgaaggg
      781 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
      841 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
      901 ttacctaaac tggccgcaac acatcattta gataatgaac aggtgtataa attcctgagt
      961 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1021 atgctgaaaa ttgttttgga cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1081 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccagg actatacctg
     1141 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt accctgtctg
     1201 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1261 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaagact
     1321 atttttaacg cattacccac tctgtttaat acgtttaaat tcgataaaaa aaatatgaag
     1381 cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc gatacagcat
     1441 aaacaacaaa acgttgtaga gacggtttat cttgctttat ctgaccatgc acgcctgttt
     1501 ggatttactg ctgaagatat tatggatttt tggcaacata aagcgccaca aaaatactct
     1561 gcctttgagt tggcttttga attggatcac cgggttattg ctgaattaat ccttaatacg
     1621 ataaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat tgcggagaaa
     1681 aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta aatctttgcc
     1741 tgcgccgata cgcataaaca tcttccacat gtccttcacg aatacggttt tgtagtgcgc
     1801 gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaagagtggc ccaatgcggg
     1861 gggcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc gagacgctgt
     1921 accacggttc gctggcaagt tcgtcttctg gatagcgcgg cggcgggatg tcgcggaaat
     1981 ttacttccgt catgtagcaa atttcatcgt aatcataaaa caccacgcgc ccgtgacggg
     2041 tgacaccgaa gtttttaaac aacatgtcgc cagggaaaat attggcggcg gcaagctggc
     2101 gaatggcatt accgtactct tcaatggcgt cgcgcagctg ctgaccttcc acttgttcca
     2161 gccagatatt gagcggcacc attcgccgtt cgatgtagag atggcgaatc acaatctgtt
     2221 cgccgagatc ggtgattttt tccggcgcct cctggagcag taattccatt aatgccgggg
     2281 aaatatgccg cttcgccagt acaaagtttt caaattcctg ggtgtccgcc attcggccta
     2341 cacgatcgtg ctctttcacc agttggtagc aggcgcggac gtgagccgca gacatctctt
     2401 tctgcggcgc gaacttgtct ttaatcactt tgaatacccg atcaaagccc ggcagcgtga
     2461 acaccagcat caccattcca cgaatgcccg gcgcttcaat aaattgctca ttacagcctt
     2521 gcagataaat cagatattca cggtaacttt cggttttggc gtgcttctgg cagccgatag
     2581 ccatatacag ttctgcggtc gttttgcccg gtagaatttc ccgtagccac tcgaccagtg
     2641 cggcaggtag cggcgcgtaa accataaaat aggaacgcgc gaagccaaag acgatgctcg
     2701 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccatcgtcc gtctggtgga
     2761 tcggcagcaa aaagggcaat gtgccggaag gcgttattag tttgcctacc agccaggcgg
     2821 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     2881 tccccagcgt ttccgtcaga tggcgaacga tgtaatgaat gtcacggctt ttattctgcc
     2941 agcgcagacg caacggcagg tcgctgataa cacgcatcag cagagattcc cagccgtgat
     3001 cggggtgaaa gtctttcgcc agcgggcggg taatggtacg aaaacggcgc tctggctggg
     3061 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3121 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac aaccgggtgt
     3181 aattctcttt aacgcgcagt aaaaatgccg cgtccgtgct ttggccgtta gtaatgcagc
     3241 gcagttgctc caccaccaga cccacgtgat gatcgtaaag atggatacgg tttttcatcg
     3301 cctgctggac ggcgtgccag tcggcttgtt cgaaacgctg ctgcgccccg gaagtcactt
     3361 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgtgca atcaataatt
     3421 ccaggccacg cggcatcgcc tctccttaat tcgcccctcg ccttacggaa aggggcgttg
     3481 gtcgttagaa ctgcgattct tcagtggaac ctgtcagtgc ggtaaccgac gacgtgccgc
     3541 cctgaataat ggtcgtcact ttatcgaaat aacctgtacc cacttcctgc tggtgagata
     3601 cgaaggtata gccatctttc gcggcggcaa attccggctg ctgcactttc tcaacgtagt
     3661 gcttcatacc ttcgccctgc gcataagcgt ttgccaggtc gaacatgtta aaccacatgc
     3721 tgtggatacc tgccagggtg atgaactgga acttgtagcc catatccgac agctgctgct
     3781 ggaagctggc aatagttttg tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt
     3841 tataagccag cagtttgcac ggatatttcg cgtggatggc ttgtgcaaag cgacgcgcca
     3901 gttccaggtc cggcgtggag gtttcacacc ataccaggtc agcatatggc gcatacgcca
     3961 ggccacggct gatcgcttgt tcaatgcccg catgagtacg gaagaagcct tcgctggtac
     4021 gctcgccggt aataaattcg ctgtcatatg ggtcgcaatc ggaggtaatc agatccgccg
     4081 catcggcatc ggtacgagca accagcaggg ttggaacgcc cgtcacgtca gctgccagac
     4141 gcgccgcgac cagtttctga atagcttcct gagttggcac cagcactttg ccgcccatgt
     4201 gaccacattt cttcactgat gccagctgat cttcgaagtg aactgccgct gcaccggctt
     4261 caatcatcgc tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat
     4321 cagcaacgat cggcaggaag taatcgacat agcgcggatc gcccggctca atgcccgcgg
     4381 accattggat ctgatccgca cggcggaagg tattgttgat ccgctccacc accgctggca
     4441 ccgagtttgc cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg
     4501 ccgctacctg ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg
     4561 cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac tcgccgtgca
     4621 gcagacgcca cattttcgcc gcgcccagtt gcgccagggt gcattcagga ttgactgaac
     4681 cgcgtaattt caccacatct tccgcgctgt aagggcgagt aatgccttcc caacgcggtt
     4741 gagtccactc tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct
     4801 ccatagttat gtggtggttt acgctaacag gcggtagcct ggcagggtca ggaaatcaat
     4861 taactcatcg gaagtggtga tctgttccat caaacgtgcg gcatcgtcaa aacgcccccg
     4921 ggagaaacgt tcttcgccca gttcgctggc aatgactttc atctcttcgc ccagcatctg
     4981 gcggaacaag gctttggtca ccggtttgcc attgctcaac gttttttgat gatggatcca
     5041 ctgccagatt gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat
     5101 cggcacgcag ccgttgccgg agatccatgc ttcgatgtac tgcacagcca cgcgaatgtt
     5161 ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt gccagcagat gatcggcagt
     5221 aatcggcgcg tcttgttcgc gcatcacttc aagctgattt ttacgggagc cgagaatgtc
     5281 gttgaatacc gccattgccg tgtccgcaag gcctgggtga gcgatccatg tgccatcgtg
     5341 accgttgttg gcttccagcg atttatccgc ttttactttg ttgagcacct ggttattgcg
     5401 ctcttcatct ttgctcggaa taaacgccgc catgccgccc atcgcaaaag caccgcgttt
     5461 atggcaggtt ttaatcaaca ggcgtgagta agcattcaga aatggtttat ccatcgtcac
     5521 tgcctgtctg tctggcagga cgcgatcggg atagtttttc aacgttttga tataactgaa
     5581 gatgtaatcc caacgaccgc agttcagacc aacaatatgg tcacgcagcg cgtgaaggat
     5641 ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc
     5701 gcgcggcaga ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc
     5761 ctgccaggac tgggttttcg gcagatagaa atagggacca ctgccctttg ccaacagtgc
     5821 ctgatagttg tggaagaaat agagcgcaaa atcaaacagg ctaccgggga ttgcctcacc
     5881 acgccaggta acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaacaccgc
     5941 tggattgggc ttgagctgat aaattttgcc tgcttcattg gtatagctga tggtgccgtt
     6001 aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg ctccagtccg gggccagtga
     6061 atcttcgaaa tcggccataa agactttcac attggcgttc agtgcgttga tcaccatctt
     6121 gcgctcaacc gggccggtaa tctctacccg acgatcttgt aagtccgcag gaataccacg
     6181 aattttccag tcagcatcac gaatggaagc tgtttccgaa ataaaatcag gcaacgttcc
     6241 gttatcaata tcttgctgct gctgaatgcg cgctgccaga agtttattgc gttgtggcgt
     6301 aaaatgcgtc accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc
     6361 ctgctcgcca tacggcctta tgaaagccag ttcatcggtt gttgttgcct gttcagtcat
     6421 cgtgcagctc ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg atcgttgtgc
     6481 gcttttcgtt cagcacaact aagact
//
LOCUS       DARNVM010000218         6504 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.218, whole genome shotgun sequence.
ACCESSION   DARNVM010000218 DARNVM010000000
VERSION     DARNVM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.218"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(224..1360)
                     /locus_tag="SI621_004352"
     CDS             complement(224..1360)
                     /locus_tag="SI621_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEI4066989.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLSEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1363..1725)
                     /locus_tag="SI621_004353"
     CDS             complement(1363..1725)
                     /locus_tag="SI621_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEI4066990.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2262..4175
                     /gene="mtlA"
                     /locus_tag="SI621_004354"
     CDS             2262..4175
                     /gene="mtlA"
                     /locus_tag="SI621_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEI4066991.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4404..5552
                     /gene="mtlD"
                     /locus_tag="SI621_004355"
     CDS             4404..5552
                     /gene="mtlD"
                     /locus_tag="SI621_004355"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEI4066992.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5552..6139
                     /gene="mtlR"
                     /locus_tag="SI621_004356"
     CDS             5552..6139
                     /gene="mtlR"
                     /locus_tag="SI621_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HEI4066993.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6151..6360)
                     /locus_tag="SI621_004357"
     CDS             complement(6151..6360)
                     /locus_tag="SI621_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066994.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1607 a         1657 c         1678 g         1562 t
ORIGIN      
        1 gagccgtggc agaggaacaa ctcatatcat cattgcgatc tcgacaatgt atagtctgat
       61 ccttcggcag atcaagacgg ataaaaaatc caatgagttc acactcatcc cgaacttctt
      121 aacataatgg atattaacga aaaatcatca cagccgatga aataatatgc aaaaataatt
      181 agtccttgca gggagcaaag ttccataatc tcccccgccc ccctcaatga tccaaataaa
      241 gataatgcat ccagctggtc attcttagca gcactttccg catcaccgaa acatggctga
      301 aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat
      361 cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg
      421 attgcaatac cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atgctagtca
      481 gtttgccgtg gaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg
      541 gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg
      601 ggatgaaaac catcaccgga cgcaacggca aggcagctgc gtatgtacct gggcggatca
      661 gtacctgagt aacgtagcca ttgctcggcg cgcgaatgac agtctgctca aggttatatt
      721 ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac
      781 tgtcgagctg gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcgtcct
      841 gcgcgaggaa gttttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg
      901 cctggctgcc tttcagataa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa
      961 cctgggtggt gttggcctgc gcttcagaga gctgcgcgcg cagcgtcttt atattatgcg
     1021 tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga
     1081 gtttaaaaag cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa
     1141 caattcccgt cacctgtggc gtgatgggga tcgctatcac tgccttttgc gcggtaaaag
     1201 tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca
     1261 gcgccgccgt cgccagcgtc cactgattca ccggaatgcg gaagatttta aagaccgccc
     1321 acgccagcgc cacgtaagtt aaaacaatca atagatccat atttagatct ccgggaacgt
     1381 ggaatagtca gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga
     1441 aagtgcggcg atttccgggt cagtcgcttt ctcttgcgac gcaacatgtg actgcatgcc
     1501 ccagccgcgc tccggttgat agagcgtcgc ccagatccac aggaacggcc agataacatg
     1561 tagagtaaac aggctcaccc agcccgccgt atgaatagcg tcggcatggg gatggttgcg
     1621 ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag
     1681 aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc
     1741 acgtttatca gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac
     1801 aataatccgg gttcgcgatt ctcgccataa caccaaagaa taattttaag aggtgatgag
     1861 ttgcttagtt acataacgat ttcatgacga aggcataaca tgctgtagat cacatcaggt
     1921 gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaat
     1981 aaaaatgtga catcactcac atttaaatgc catttttagc gaaaatcgcc gccttgttgc
     2041 ttttttacac aagagttttg tgatgagcat cacgtcaatt acctctctac cccctatatt
     2101 tatgtgactg atatcacaca aaaggccgtc gactggacag ttaaccgatt cagtgccaga
     2161 tttcgcagta tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc
     2221 ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga
     2281 tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt
     2341 ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga
     2401 cgctggcgaa gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata
     2461 ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatt accaccatgg
     2521 gcgttatcgt cggcgcagac atgccgatgt tcctaggttc tatgattgca ggtccgctgg
     2581 gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg
     2641 agatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat
     2701 tcctcggcat tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact
     2761 tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc
     2821 tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggcatt cagcagtccc
     2881 atgaactggg taaatccatc ttcttcctga ttgaagctaa cccaggtccg ggtatgggcg
     2941 tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg
     3001 caatcatcca cttcctgggc ggtatccacg aaatctactt cccgtatgtg ctgatgaatc
     3061 cgcgtctgat tctggccgtt atcctcggtg gtatgactgg cgtgttcacg ctgactatcc
     3121 tgggcggtgg tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga
     3181 caccaaaagg tgcatacttc gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct
     3241 tcgttgtctc tgccattctg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag
     3301 cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa aggcgcgtct ccgctgtctg
     3361 cgggcgatgt gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgatgccg
     3421 gtatgggttc cagtgcgatg ggcgcaggtg tgctgcgtaa gaaaattcag gatgcaggtc
     3481 tgtcgcagat ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctgg
     3541 tcatcactca ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcacagcata
     3601 tttcgctgac caacttcctc gacagcggcc tgtacaccag cctgaccgaa cgtctggttg
     3661 ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg
     3721 atgattccag tgctaacctg ttcaagctag gcgcggagaa catcttcctc ggtcgtaaag
     3781 cggccaccaa agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg
     3841 ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg
     3901 gtgagtccat cgcggtaccg cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg
     3961 gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg
     4021 cccgtctggt gattggtatt gctgcccgta acaacgagca cattcaggtt atcaccagcc
     4081 tgaccaatgc actggatgat gagtctgtca tcgagcgtct ggcacacacc accagcgtgg
     4141 atgaagtgct ggaactgctg gcaggtcgta agtaatccaa tcccacctct ccacatggag
     4201 aaggtggggc ttattgcctg atgcgctacg cttatcaggc ctacaaaaca aagcgtagtt
     4261 tgttgaattt gcacgttctt gtaggccgga taaggcgttt acgccgcatc cggcgcggcc
     4321 cctctccaca tggagagggt ttgggtgagg gaaaagcctc accccagccc tctcgggtaa
     4381 aaacattgat gaaggttaat actatgaaag cattacattt tggcgcaggt aatatcggtc
     4441 gtggctttat cggtaaactg ctggcagacg cgggtatcca actgacgttt gccgatgtca
     4501 atcaggtggt acttgatgcc ctgaatgccc gtcatagcta tcaggttcat gtggtcggtg
     4561 aaaccgagca ggtagatacc gtttccggcg tcaatgccgt cagcagcatt ggtgatgatg
     4621 tcgttgatct gattgctcag gttgatttag tcactaccgc cgttggtccg gttgtgctgg
     4681 aacgtattgc cccggcaatc gccaaagggc tggtgaaacg taaagaacaa ggtaatgaat
     4741 ccccgctgaa catcatcgct tgtgaaaaca tggtacgcgg caccacgcag ctgaaaggcc
     4801 atgtgatgaa cgccctgccg gaagatgcca aagcgtgggt agaagaacac gttggctttg
     4861 tcgattccgc cgttgaccgc atcgtgccgc cttcggcttc tgcaaccaac gatccgctgg
     4921 aagtgacggt agaaacattc agcgaatgga ttgttgataa aacgcagttc aaaggcgcgc
     4981 tgccgaacat cccaggcatg gagttaaccg acaacctaat ggcatttgtc gaacgtaaac
     5041 tcttcaccct gaacacgggt catgctataa ccgcgtacct cggaaaactg gccggtcatc
     5101 agaccattcg tgatgcgatt ctcgacgaga aaatccgcgc ggtggtaaaa ggtgcgatgg
     5161 aagaaagcgg tgcggtactg atcaagcgct acggctttga cgcggacaaa catgcggcgt
     5221 acatccagaa aatcctcggt cgtttcgaga acccgtatct gaaagatgat gtagagcgcg
     5281 taggccgtca gccgctgcgt aaactgagtg ctggcgaccg tctgatcaag ccactgctcg
     5341 gaacgctgga atatggtctg ccacataaaa acctgattga aggtattgcc gctgcaatgc
     5401 acttccgcag tgaagatgat ccgcaggctc aggaactggc agcgctgatt gccgacaaag
     5461 gcccacaagc cgcgctggca cagatttccg gtcttgatgc caacagcgag gttgtatccg
     5521 aggcagtaac cgcttataaa gcaatgcaat aatggtggac caggcgcagg ataccctgcg
     5581 cccgaataac agattgtcag atatgcaggc aacaatggaa caaacccagg cctttgaaaa
     5641 ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga agctttctga tcaccgccgt
     5701 cgagcttttg accgaggcgg taaatcttct ggtgcttcag gtattccgca aagacgatta
     5761 cgcggtgaag tatgctgtag aaccgttact cgacggcgac ggtccgctgg gcgatctttc
     5821 tgtgcgttta aaactcattt acgggttggg tgtcattaac cgccaggaat acgaagatgc
     5881 ggaactgctg atggcattgc gtgaagagct aaatcacgac ggcaacgagt acgcgtttac
     5941 cgacgacgaa atccttggac cctttggtga actgcattgc gtggcggcgt taccaccgcc
     6001 gccacagttt gaacctgcag actccagttt gtatgcaatg caaattcagc gttaccaaca
     6061 ggctgtgcga tcaacaatgg tcctttcact gacagagctg atttccaaaa tcagcttaaa
     6121 aaaagccttc caaaagtaag caacgcctgc ttactgcccc tctacctgct tcggccgata
     6181 aagccgacga taatactcca gacgttgtag atataacggc acgctttcat caggtattcc
     6241 ggacggaatc gcgttacggg gaggtagttt tttcagatac tcccggaacg cctggctcga
     6301 tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta atttgcccat
     6361 gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt agtttagatc
     6421 ccggtataaa tgacattaag gaaaacgtgc tcaatcctca ataatcatcc gcttttgtta
     6481 actatctttt ttctttaaat ttct
//
LOCUS       DARNVM010000219         6425 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.219, whole genome shotgun sequence.
ACCESSION   DARNVM010000219 DARNVM010000000
VERSION     DARNVM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.219"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            134..1318
                     /locus_tag="SI621_004358"
     CDS             134..1318
                     /locus_tag="SI621_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HEI4066995.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1429..2352
                     /locus_tag="SI621_004359"
     CDS             1429..2352
                     /locus_tag="SI621_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEI4066996.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2356..3174)
                     /gene="nlpA"
                     /locus_tag="SI621_004360"
     CDS             complement(2356..3174)
                     /gene="nlpA"
                     /locus_tag="SI621_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HEI4066997.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            3384..3677
                     /locus_tag="SI621_004361"
     CDS             3384..3677
                     /locus_tag="SI621_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4066998.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            3979..4476
                     /locus_tag="SI621_004362"
     CDS             3979..4476
                     /locus_tag="SI621_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HEI4066999.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            4486..4668
                     /locus_tag="SI621_004363"
     CDS             4486..4668
                     /locus_tag="SI621_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067000.1"
                     /translation="MNINGKNASFTPEELIRKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            4724..>6425
                     /locus_tag="SI621_004364"
     CDS             4724..>6425
                     /locus_tag="SI621_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /protein_id="HEI4067001.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSNKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVVTQKSAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARSYTTWTKVLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLAGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMVTLMIGATLICSVAISEL
                     VKALSKTAAEEITKEITDSIKSTVESMIKSVSKKIMSALNFLGDKIKASSQFIMIFAK
                     ITILLEKIASGVCTIITSSMNQYAASKSADIEILQQDISNLSKTREQMLTVMQRIDKT
                     V"
BASE COUNT         1843 a         1410 c         1373 g         1799 t
ORIGIN      
        1 ctggccctat aatattgcgc tagcattgct ttctggttgt atcagcgata ctcaaaaatt
       61 cttaatacaa tactcattcg actggtactt atttgtaact cagttatatt ttgtcgcccg
      121 gtgattcaga agaatgcaaa aaacggctac cactccatca aaaatacttg atctcactgc
      181 cgcggcattt ttacttgtcg cctttctgac gggtattgcg ggcgctctcc agactcctac
      241 cctaagtata ttcctcgcag atgaactgaa agcccgtcct ataatggtag gttttttctt
      301 caccggtagc gctattatgg gaattcttgt cagtcaattt ctggcaagac actccgataa
      361 acaaggcgac cgtaaattac tgatcctgct atgttgctta tttggagtgc tggcctgcac
      421 gctttttgcg tggaatcgca actacttcat tctcctctct acgggcgtac ttctgagtag
      481 ttttgcttcc accgcaaacc cgcaaatgtt cgccctcgcc cgtgaacacg ccgacagaac
      541 aggccgtgag acggtaatgt tcagtacatt tttacgtgct cagatctcgc ttgcctgggt
      601 tatcgggcca ccgctcgctt atgagctggc aatgggattt agttttaaag tgatgtatct
      661 caccgcagcc atcgcatttg ttgtttgcgg gctgatagtc tggttgtttt tgccatcaat
      721 acaaagaaat attcctgtcg ttacccaacc cgtagaaatt ttaccctcca cccataggaa
      781 gcgggataca cggctacttt tcgtggtttg ttcaatgatg tgggcggcga ataatctcta
      841 catgataaat atgccgctat ttattattga tgaactgcat ctaaccgata aactggctgg
      901 agaaatgatt ggtattgctg ccggtctgga aattccgatg atgttaatcg caggctatta
      961 catgaaacgt attggcaagc ggctattaat gctcattgct atcgtgagtg gaatgtgttt
     1021 ttacgccagc gtactcatgg cgacgactcc ggcagttgag ctggaattgc aaattctaaa
     1081 tgccatcttc cttggtattc tctgtggtat cggcatgctt tattttcagg acctgatgcc
     1141 tgaaaaaata ggctctgcga caacgttata tgcaaatact tcacgcgtcg gctggattat
     1201 cgccggctct gttgacggaa ttatggttga aatctggagc taccacgcgt tgttctggct
     1261 ggcgataggg atgttgggta ttgcgatgat ttgcctgctg tttattaaag atatttagct
     1321 cacctgtctg ggctaagaaa aatcggagtg gcgcaaacag agccatcctc gccatttttg
     1381 ctatcatgcc tgcatacata aacgacaaaa cagtatgcag agggaaaaat gggttccacc
     1441 agaaagggga tgctgaacgt tctgattgcc gccgtgttgt ggggaagttc aggggtctgc
     1501 gcgcaataca tcatggagca aagccagatg tcgtcgcagt ttttgactat gacgcgtttg
     1561 atattcgccg gtttgattct gctgacgctg tcatttgttc atggcgataa aatcttttct
     1621 attattaaca atcacaaaga tgccattagt ctgctgattt tttccgtggt tggcgcgcta
     1681 accgtacagc tcactttttt gctaaccatt gaaaaatcga atgctgccac ggcaacggtg
     1741 ctgcaattcc tctcaccgac gattatcgtc gcctggttct cactggtgcg taaatctcgc
     1801 ccgggcattc tggttttctg cgctattttg acatcgctga tcgggacttt tttattggtg
     1861 acacacggta atccgacgtc attatcgatc tctcctgccg cgttattctg gggcattgcc
     1921 tcggcatttg ctgcggcatt ctataccacc tatccctcaa cgctaattgc ccgctatggc
     1981 acgttgcctg tcgtcggctg gagtatgctg atcggcggtc taattctgtt gcctttttat
     2041 gccagacaag ggacaaactt tgtcgttaac ggcagtttga ttctggcgtt tttttatttg
     2101 gtggtcattg gtacgtccct gacatttagt ctgtacctga aaggagcaca attaattggc
     2161 gggccaaaag ccagcatttt gagctgtgca gagccattaa gtagcgcgct gctctctttg
     2221 ctgttgctgg ggatcacatt cacattaccg gactggctgg gcacactgct gattctgtca
     2281 tcggtgattt tgatttcaat ggattcccgt cgccgcgcca gaaaaataaa tcgtccggcg
     2341 cgggatgagt gagaattacc agccaggcac cgcgccaccg ttaaagatgg tttctgccgc
     2401 tttagcgact tcggatgatt gataagattg cagaaattcc ttcacgtttt ctgcattctt
     2461 attatcttcc cgtgctacca gaatattcac atacggcgaa ttcttatctt caataaatac
     2521 gctgtcgtgc accggagaaa gcccggtctg ctgaatgtaa gtggtgctga taatcgccac
     2581 atcaactttg ggatcatcca gtacgcgcgg gagctgcgcc ccctcgagtt ccataatctg
     2641 caaatgacga ggattctcgg ttatatccag cgcggtaggt agtaaccctt ttccctcttt
     2701 cagagtaatt agcttctctt tttgcaacag taaaagcgcg cgaccaaggt tggtcgggtc
     2761 gttggggatc gccactgtcg cgccttcttt tatttgcgcc acggttttga tttttttgga
     2821 ataacccgcc atggggaaga caaacgtatt tcccaccgct accagtttat aaccatgcgc
     2881 ctgattatct tgttcaagaa agggtcgatg ctggaagacg ttcgcatcaa gctcgccatg
     2941 atttgtcgca tcgttcggta gcaacgaacc gctaaatcct accaattcaa catcgaggcc
     3001 atatttctct tttgccacct ttttagcgac ttccgcgaca tcttgttctg cgccattaat
     3061 aacgcccact ttaatgtgct tttcatcgct gctgctctgg tcgcaacctg ccagcagaat
     3121 tcctgccagt aataatgcgg cccccgcccg tagatgatgt gttgtcagtt tcactttttt
     3181 atcctttttt taatcgttaa ctgactataa tggcaagatc actccgattt aaaaaacgaa
     3241 aagctatcga atagaagaaa aaggtatata aaactggcac ttcttactga ccacgcaaac
     3301 acctttcgta cagcatgatg agagcggcaa aagcaaacta cacttatgct tcattcgatg
     3361 cacatttgca gaaggttgcc gctatgaagc caacgatgct actcatgatt accgtattcc
     3421 tgactttccc agccatttct caggccgaat cgccattcag ttctctacag tcggcaaaag
     3481 aaaagaccac ggtattgcaa gatttacgta aaatttgcac gccacaggcg tcattatcag
     3541 acgaagcgtg ggaaaaatta atgttgtctg atgagagtaa caaacaacat atccgcgaag
     3601 ctattgtggc gatggagcgt aataatcaga ataactactg ggaagcgctg ggtaaggtgg
     3661 aatgcccgga tatgtgaatt tcgcacacct ccgcgctcag gccagcagcc gctgacttaa
     3721 gctcgcacat cagtaaatca cctgcttttt caaattccaa tcaaaattgc accagttcat
     3781 caggattgtg gataaatttc cctctacatt gttttcattt catcacatag aaaatcttat
     3841 ctcattgaaa caatacttgt ttttaatgca gaaaaggaag cccaacggat gggcataaaa
     3901 atgagcgaat tttgatatca cccccctcta aaaaatggtg aagtctgaac aaagaatgtt
     3961 cgaaaggaga gagtagagat ggaaagcgca aatctggatt tagaagaaaa taaagaaatt
     4021 gccagtaagt tcgaacgtgc attaggtatg ggagcaacac ttgcagagct gcatggcatt
     4081 actccggata cccttgaagg cgtttatgct tacgcttata acttttatga aaaaggaaga
     4141 cttgacgaag ctgagctctt ttttaaattt ctctgtattt atgatttcca aaattataac
     4201 tatttaaaag gatatgccgc cgtttgtcag ttgaagaaag attatcaaaa ggcattcgac
     4261 atgtatcata tctgtttaat gctatctccc gataacgatt tctctttggt ttattatatg
     4321 ggccaatgcc agatgggctt aaaaaacatt aaaatggcca ctgagttatt taatacagtc
     4381 gtaacctaca gccaaaatga aaaagttaaa gagatggcta ctgcttatct tgaactcttg
     4441 acagccaata ccgaagagga agttcaaaca gagtgagtcg gcaaaatgaa cataaacgga
     4501 aaaaacgctt cttttacgcc tgaagagtta atcaggaaat atcatgagga agccccggtt
     4561 gatttaaaaa ataaccatgg cgataaaata aatgagaaga ataacaataa acacattact
     4621 tactttcccg ttgatgccat tttagatggg aataaacatc tgccatgagt taaataaaaa
     4681 agaattaatt acacccgatg atagcttaaa aaggaggtga attatgtcgg caccgattac
     4741 gggacaaacg atcacgtttg aacaaatcag cgaaacattg cgcacacagt atagcgatgc
     4801 ggaaaaaaga ctgcaggaca gcaacaaaac gcaagttgat cccatgcggc ttaataaaaa
     4861 cccgaaatcc cttgataatg atattcgcgc tcgtcttgaa aacaaaccga tgcttgctcc
     4921 gcctgcaatg caggtttcag attccgacaa cgccactaca gcaaagacga acgatgcccg
     4981 cctgacaatg attttgggta atctaacagg cattgccgat caggatatta caacacgttt
     5041 gcataataac cttgacagca ccctgcttcg acacgaaatg gcacataata agtttcgtga
     5101 attaagtgac gcttactcat cgtcattaga ttccgcgcaa aaagccgacg acatcatgca
     5161 tcaggccaat aataattaca atgcggtcga taaaaaggtg caatcgctgg agaaaaaagt
     5221 caacacattg aatcaagagc tctcgcaact gcaacctggc gatccgcaat ataataaagt
     5281 agtgacgcaa aaaagtgcgg ctgaaaaaac actcacgctg tcattacaaa aaaaatcgtt
     5341 agctgagaaa tcgttaaata cagccattat ggatgctgat gctgcgatcg gccaaagtat
     5401 ggaaattttt gacgaaattc aacagcaaga acagattaat aacttcacca ccaatatttg
     5461 cctgacgcaa gaaaaccaga aaaacagaaa cgcgacagcc acatttatcc ttttgattac
     5521 ctcagtaatg gaagttattg gtgataccaa ttgcgactct attaaaaatc aatctgaggt
     5581 aatgaaagag attaaccatg tcagggaaaa taaactcaat gaaacagcgc gtagctatac
     5641 tacatggaca aaagttttaa acattattaa tgaggtcgtt actgtagtta catttgccgt
     5701 aagtgcagcc ttaattgtga ttggtattgt cgcagcagtt ccaagtgctg gggcaagctt
     5761 tgctggagcg ctggcactaa ttggaggaat cgcgggggca gtagtattag gtgttgatat
     5821 tatttgtcaa atcactctgg gtaccacggc taccggttgg attttgggaa aactaactga
     5881 aggtcttgcg ggtattattt ctaaatatga ccccacacta ctcgcaatct gcgctcttct
     5941 ggacgccttg ggtgtccaac aaacgactat tgatttagta caagatattt actcatctgc
     6001 agccgcttct attgttatgg taacattaat gattggtgca acattaattt gttcagtagc
     6061 aataagtgaa cttgtaaagg cactctcaaa aactgccgcc gaagaaatca caaaagaaat
     6121 aactgacagt ataaaatcca ccgtggaatc tatgatcaag tcagtatcaa aaaaaatcat
     6181 gtcagcatta aattttctcg gtgacaaaat aaaagcatca tcacaattta taatgatatt
     6241 tgcaaaaata actattcttc ttgaaaaaat tgcctcgggc gtttgtacaa taattacatc
     6301 gagcatgaat caatacgctg ccagtaagtc tgcagatatc gaaattctac aacaagatat
     6361 aagcaatcta tcaaaaacgc gcgaacaaat gcttacagtg atgcaaagga tagataaaac
     6421 cgtcg
//
LOCUS       DARNVM010000220         6416 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.220, whole genome shotgun sequence.
ACCESSION   DARNVM010000220 DARNVM010000000
VERSION     DARNVM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.220"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            10..918
                     /locus_tag="SI621_004365"
     CDS             10..918
                     /locus_tag="SI621_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEI4067002.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1138..3129)
                     /gene="tkt"
                     /locus_tag="SI621_004366"
     CDS             complement(1138..3129)
                     /gene="tkt"
                     /locus_tag="SI621_004366"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEI4067003.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3407..4165
                     /gene="loiP"
                     /locus_tag="SI621_004367"
                     /gene_synonym="yggG"
     CDS             3407..4165
                     /gene="loiP"
                     /locus_tag="SI621_004367"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HEI4067004.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4595..5515)
                     /gene="speB"
                     /locus_tag="SI621_004368"
     CDS             complement(4595..5515)
                     /gene="speB"
                     /locus_tag="SI621_004368"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEI4067005.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5651..6382)
                     /locus_tag="SI621_004369"
     CDS             complement(5651..6382)
                     /locus_tag="SI621_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067006.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1554 a         1708 c         1682 g         1472 t
ORIGIN      
        1 aagattcata tgaaagcacc tgtttcaata acctcatttc gcccacaaaa atctattgct
       61 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      121 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgagccgggg
      181 ctgggtttta agatcccttt tatggaaagt gtagagaaaa tttccacgcg taatcaggct
      241 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacggta
      301 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacacc
      361 attgaagcat taaaagatcg tctgattgtc cgtcagttgc caactcaact ggagaacgtt
      421 ttcggccaat ataccgccat cagcgcggta caggatcgta caaaactggt tcaggatctg
      481 caaaatgcaa tgcgtaaagc cgttgttggc ccggtggtga ttgatggtgt ccagattgaa
      541 aatattgatt tttctgacgc ttatgaaaag tcgattgaag accgaatgaa agcagaagtc
      601 gccattgcca cacgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcagta
      661 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agctgagacc
      721 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
      781 gaagctatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt tgccctcacc
      841 actgctgagc gttgggacgg taaactgccc gataccatga tccccggcag tacagttccg
      901 tttatctcta ctaagtaaaa aaagggccgc tttcgcggcc cttgagactg atagcaaaga
      961 caaaactgcc tgatgcgcta cgcttatcag gcctacatac ttcctgcaat atgttgaatt
     1021 tgcaaaatct tgtaggacgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     1081 tttgccaaca atctgaaggg ccgcatttgc ggcccttctc acaaagcatc ttaccgatta
     1141 cagcagcgct tttgctttcg caacaacgtt gtcaacggtg aagccgaact cttcaaacag
     1201 ctgctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcac cgttcaggcc
     1261 aacatacttg taccagtagt cagcaatgcc agcttccacc gcaacacgag cggtaaccgc
     1321 tttcggcagt acggattcac ggtaagccgc gtcctgcttg tcgaatgcgt cggtagacgg
     1381 catggaaacc acgcgcgctt tcacgccttc ggcagtcaat ttttcgtatg ccgctacagc
     1441 cagttcaact tcagaaccgg tcgcgatgaa gatcagttcc ggctgaccag cacagtcttt
     1501 cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt cctgctgcgc
     1561 caggttctga cgggagagga taagcgcagt cgggccgtcc tgacgttcaa cgccgtattt
     1621 ccacgctacc gcggattcaa cctggtcaca cggacgccat gtagacatgt tcggggtcac
     1681 gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat
     1741 ggagtcgtgg gtgtaaacca tcacctgacg ctgtttcatc agcgcagcca tacgtacggc
     1801 attacgtgcg tattccacga acatcaggaa ggttgaggta tatggcagga aaccaccgtg
     1861 cagggagata ccgttagcaa tcgcggtcat accgaactcg cgaacaccgt agtggatgta
     1921 gttacccgca gcatcttcgt tgattgcttt agaaccagac cacagggtca ggttagacgg
     1981 cgccaggtct gcagaaccgc cgaggaattc cggcaacagc ggcccgaacg cttcgatagc
     2041 attctgagac gctttacggc tggcgatctt cgccgggtta gcctgcagtt tagcaatgaa
     2101 ctctttcgct ttcgcgtcga agtcagacgg catttcgcct ttcatacggc gggtaaattc
     2161 agcggcttcc tgcggataag ctttcgcgta agcagcgaat ttctcgttcc atgcggactc
     2221 tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg ggatttcgaa
     2281 cggcgcgtat ttccagccca gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg
     2341 cgcaccgtgg gagtcgtggg taccggcttt gttcggggaa ccgaaaccga tgatggtttt
     2401 gcacatcagc agggacggtt tgtcggtcac tgcgcgcgct tcttctactg cgcgtttgat
     2461 ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg
     2521 cattgcggtg tcgtcggtaa accagccttc aacgtgacca tcgatagaaa taccgttgtc
     2581 atcgtagaac gcaatcagtt tacccagctt caacgtaccc gccagagagc aaacttcgtg
     2641 ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt ggtcgacgat
     2701 gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg ccataccgac
     2761 tgcgttggca ataccctgcc ccagcggacc ggtggtggtt tctacacctg cggtgtaacc
     2821 cacttccggg tggcccggag ttttagagtg cagctgacgg aagtttttca gttcttccat
     2881 cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc cgtggccgtt
     2941 ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt gtttcaggaa
     3001 atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg ggtgaccgga
     3061 tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa gctctttacg
     3121 tgaggacatt ttgactccag atcggatgat gaagggcaca cccttaacga cttgacgaca
     3181 gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt
     3241 acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat gttgcgcaat
     3301 ctattcgccg gtccactgca cttttcctta tacttcgatt gagcgtcaat tcacctgcag
     3361 atggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc
     3421 cttattggta gcaatgagcg tggcaacggt actgaccggt tgccagaata tggactccag
     3481 tggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg atgcgcaggt
     3541 gaaaaccctg agcgatcagg catgtcagga tatggacagc aaggcgacga ttgcgccagc
     3601 caatagcgaa tacgctaaac gtctgacgac aatttctcgc gcgttaggcg ataacatcaa
     3661 tggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc
     3721 taacggctgt atccgcgtct atagcgggct gatggatatg atgacggata acgaagtcga
     3781 agcggtgctc ggtcacgaaa tggggcacgt ggcattaggc catgtgaaga aaggaatgca
     3841 ggtagcactt ggtacaaacg caattcgcgt tgcggcggcc tctgcaggcg gaattgtcgg
     3901 cagtttgtca caatcacaac taggagacct gggcgaaaag ctagtcaatt cgcaatttac
     3961 ccaacgtcag gaatcagagg ccgatgatta ctcctacgat ttgctgcgcc aacgcggcat
     4021 cagtccggct ggccttgcca ccagctttga aaaactggct aaactggaag aaggtcgcca
     4081 aagttcaatg tttgacgacc acccagcatc cgccgaacgc gcccaacata ttcgcgatcg
     4141 tatgagcgcg gatggagtta agtaaggcat ggtggtgcca aacgtaggtc agataaggcg
     4201 tttacgccgc atctgacatg aatggcacat ttgttatcct gtgcgcgttg ccggatgcga
     4261 tgctggcgca tcttatccag cctacgggtg ccgaacgtag gtcggataaa gcgctcacgc
     4321 cgcatccgac attaatggca catttgttat cctgtgcgcg ttgccggatg cgatgctgac
     4381 gcatcttatc cggcctacgg gtgccgaacg taggtcggat aaggcgctca cgccgcatcc
     4441 gacattaatg gcacatttgt tatcctgtgc gcgttgccgg atgcgatgct ggcgtatctt
     4501 atccggccta cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta
     4561 atggcacatc acccgtgcgc ctcttatctg gcgcttactc gccctttttc gccgcctgaa
     4621 tatacagcat ctccagcgcc agcgtcgccg cagccagagc ggtgatttcc gactgatcgt
     4681 atgccggagc cacttccact acgtccatcc caacgatgtt gagatctttc aggccgcgta
     4741 ccagtttaat ggcgcgatcg gaggtcaggc cgccaattac cggcgtaccg gtgcctggtg
     4801 caaaagcagg atccaggcag tcgatatcaa aggtcaggta aaccggcata tcacccacaa
     4861 tctgtttcac ctgggcgata atgtcatcca cgctgcgatc gttcacctgg caggcgtcca
     4921 gcacggtaaa gccgttgtct ttatcaaact cggtacgaat accaatctgc acggaatgat
     4981 tcgggtcgat cagaccttct ttcggcgcgg tatagaacat ggtgccgtgg tcaaattcac
     5041 aaccgttcgc ataggtatcg gtgtgggcgt caaagtgtac caacgccatt ttgccgaaat
     5101 gcttcgcatg agcgcgcagc agcggcagcg taacaaagtg gtcaccaccg aaggagagca
     5161 tacgcttacc ggcagccagc agcttctcgg cgtgcgcctg cagcttctcg ctcatctcac
     5221 gggcatcgcc gaaggcatat accagatcac cgcagtccac gacgttcagg cgctcacgca
     5281 tgtcgaaatt ccacgggaag cggttgtgtt cccaggccag attcgtcgaa acctgacgga
     5341 tcgctgccgg accgtgacga ccacccgcac gaccagaagt ggccatatcg aacggcacgc
     5401 cagtaatcac ccagtcagca tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa
     5461 aaccaaaggc gttggaaacc agtgagttat cgtattgatg acctaaggtg ctcatggatg
     5521 gactcctttt acaaagtcga tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa
     5581 gggttggatt tgcgaaacac aagtttgtgc taattatcgc cctaaatggg caaggcatca
     5641 agcaaggtta ctatttccga cacggtggcg gcgcatagcc attgttatct gccggaaacg
     5701 cttgtccaga gcacggcggc agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa
     5761 agccgattac accggtatta cgcacatcgc cttgcgccga attggctgcg taggaatcag
     5821 caacgttggc gaactgaaaa gccgcttcgg tatgtttatc tttgcggaat cctttaatcg
     5881 ctaacgaatc tccggcattc acaatataac cgttgttatt cagcgaacct tgcttgccgt
     5941 tcagcacatc caggccatca accgtagcaa cgatctcgta gttggtgtta cgactgtaat
     6001 tacggatata taattgataa ttcattccga ttctggcatg gacaatatag cgtggttcat
     6061 tattggatga atcataaacc cgcgtaatag gtagagaatt aaaattacca tcgcgaatag
     6121 catattccag ttcgttaata cgaatactat aaacgcggtc gtagccggaa ggaacattag
     6181 tcgaataact aataacgatc atctctgccg gatcgcgcca ggcgcgttca gcagatacgc
     6241 cctgaaccgt tgagtgcaca tccccacccc aggcggtgcc gacattgcct gatgcactgc
     6301 tattatactg cgcattactg gaacaaccgg ccagcagcac ggctaatgct atcaacgcgc
     6361 tacgtaattt ccattttttc ataaccgttc cctctgcatt taattaatgc agcgat
//
LOCUS       DARNVM010000221         6355 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.221, whole genome shotgun sequence.
ACCESSION   DARNVM010000221 DARNVM010000000
VERSION     DARNVM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.221"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(492..1349)
                     /gene="sitD"
                     /locus_tag="SI621_004370"
     CDS             complement(492..1349)
                     /gene="sitD"
                     /locus_tag="SI621_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HEI4067007.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNS
                     HTQDLLSPN"
     gene            complement(1346..2203)
                     /gene="sitC"
                     /locus_tag="SI621_004371"
     CDS             complement(1346..2203)
                     /gene="sitC"
                     /locus_tag="SI621_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HEI4067008.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2200..3027)
                     /gene="sitB"
                     /locus_tag="SI621_004372"
     CDS             complement(2200..3027)
                     /gene="sitB"
                     /locus_tag="SI621_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HEI4067009.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3027..3941)
                     /gene="sitA"
                     /locus_tag="SI621_004373"
     CDS             complement(3027..3941)
                     /gene="sitA"
                     /locus_tag="SI621_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HEI4067010.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4528..4962)
                     /gene="uspF"
                     /locus_tag="SI621_004374"
     CDS             complement(4528..4962)
                     /gene="uspF"
                     /locus_tag="SI621_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HEI4067011.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5103..6236)
                     /gene="ompN"
                     /locus_tag="SI621_004375"
     CDS             complement(5103..6236)
                     /gene="ompN"
                     /locus_tag="SI621_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HEI4067012.1"
                     /translation="MKSKVLAFLIPTLLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1749 a         1502 c         1503 g         1601 t
ORIGIN      
        1 taaccttcat aatctgacct attcaattga ttccagtaca ccaatgggta ttttggtgtt
       61 atggtatgtg actccaaata aaatggagcg tgaactgata gttgagcgaa caaaagtcgg
      121 actagaaacc gctcgggtac agtggcgaat tggagttggc gacccaaact gacctaggaa
      181 caatcgttgc agatcggacg attaatttca tcagaaattc ctcgctaaaa ggtggctatc
      241 actgtgatgt taacgtatct aatttgtaca agaaatttct gcgggggaaa atgaaaccgc
      301 agactcgttg tatgcaaaaa cgtgctgcgg ctaactggtg aactttcgat agtgcgggta
      361 ttgaatgatt tctaggcgtt atcgatttta cgtatttttt gcatgataga attcgtacct
      421 cctcccaacg accttccatg gctttccgga cttctgtagg ccggcggacg atgctgtcct
      481 cggatgttgt gctaattggg tgataacaga tcctgtgtat gggagtttcc ctttgtgcgt
      541 gcgttgatgc tggtaacggt aaacgtcatg atgaacacga ccgcgaataa gacgacaatc
      601 gtcggagctg gtgcgctgtc gataaaaaag gacaaccaga caccagtcat tgacactatt
      661 accgatatgc cagttgccag taggagcgca atatgaaagc gttgtgtcag gagtaccgca
      721 atggcgccag gcgcaatgag cagagaaatg gagagaatga tacctactgc tttcagcgtc
      781 gccacaatgg tcagagagac catgcacagc agcccataat gcagccatct cgtatgcaaa
      841 ccgcttacct gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg ccattttacg
      901 ctaataacaa gtgtaaccag cccggcaata atcatcgtct ggattatatc gccgatggtt
      961 atcccgagca tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt gactgcaata
     1021 tacaagatca gacctgcggc aaacatgcca gaaaaaacaa tccccatcac tgtatcttgc
     1081 ttgattcggc tgttgtcttt caggtatcca gtcgccaccg cgcagaatac tccggcgacg
     1141 aatgcccctg tagccaaagg taaccccaga atccaggcaa gcacgatgcc aggaaaaacc
     1201 gcatgactca tcgcgtcacc catcagtgcc caacctttca ggaccagaaa gacagacagc
     1261 agcgcacagg gtatggaaac taccagtgaa atcagcagtg cgtgactcat aaaggtgaac
     1321 tgcagtggct ccagtaataa tgccatcatg agcgatcctc cagcgcctta tgcgcacgcc
     1381 gacgatttgc caaaagccca tgggtgggcg caaagacaaa tgctaaaaga aacagcagtg
     1441 tctgcgccac cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc
     1501 cgaggaaact ggttacactg ccaatagtta cagcaatcat gagcaggcgg ggaaaacgat
     1561 cggttaacaa ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaaggccc
     1621 caaccgtttg cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggagtttta
     1681 atctgcccgg atgcaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa
     1741 gatctttcca tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca
     1801 ggatatccgc cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacag
     1861 atgtcgggtt caatgacacc ataaacagcc ccaggccaaa aaaggatgag aagatcaggc
     1921 cgatgatggc atcttctttt aagcgggtgc gttggttaag aaacagcatg ctgcccgcag
     1981 ccagtccgcc ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg
     2041 caacacccgg gacaatagag tgcgacaacg catcgccaat cagcgaccag ccttttagca
     2101 tcagatagca tgacaggaaa gcgcagagac cgccgaccat cgccgagacc cacatcgcat
     2161 tgagcatata ttcatagctg aagggttcca gtagcacgtt catctttctt ccctctgcac
     2221 cgctgacggt cgatgagcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga
     2281 gccgttaagt gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt
     2341 aaaagtggtg tctgtcggcc cgcttgccag gacggtgcct ttgaccatga ccgtataatc
     2401 gcaaaatgtc gtgacggatc cgagattgtg tgttgagaca agcatcgttt tgccttccgc
     2461 gcgtaactcc ctcaacaggc tgatgatttt ggcttcggtt ttcacatcaa cgccagtaaa
     2521 aggttcatcc agcaggatca catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg
     2581 tttcttttgt ccaccggaaa gctcgccgat ttgtcgatgg cgaaaattca ccatatcgac
     2641 acgttccagc gcatcggtga caatttgacg atccctcttt ttggcgatac gcaacatacc
     2701 catatgccca tagcggccca tcatcaccac atcttccacc agtacaggaa atgaccagtc
     2761 aacctcttct gactgaggga cgtaggcaac caggtttttc tgtaacgcct gtcgcgtggg
     2821 aatacccaga acagatatct ttccgctggt cagacgcaca aatcccataa tcgctttaaa
     2881 caacgtcgat tttccggaac cgtttacccc aaccagagcg gcaatagatc cacttggtac
     2941 tgtgaaggat gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt
     3001 aacgacaatg cctgcagatt gcatcattat ttctcccttt tcccggcttt aattccctgt
     3061 accagcgtac tggtagtaac cttaagaagg tcgatatacg tcggtaccgg gccgttttct
     3121 gtgctcaggg aatcgacata gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc
     3181 tgacgcgctg gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc
     3241 ttaactatat caacaacctt acgtacctgt tgcggtgttc cttgttgatc ggcattaatc
     3301 ggccacagat aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca
     3361 ctggtgacca tccaccgctg attctcaggg agttccgtaa tctgcttacg caggggggca
     3421 agggtttggg taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcatta
     3481 gcgggatcgt attttatcaa cgcatcacga atattatcga cgtaaatcag agcattatct
     3541 ggcgacatcc atgcatgggg gttaggtttg ccctcatagg ggccttcggt gatccctact
     3601 ggcgtcacac ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt
     3661 tggaaccaca attccagatt cataccattg gcgaggatca attgtgcccc ctgcgcacgt
     3721 ttaatatcgc caggggtagg ctgatactca tgaatttctg caccgggctt ggttatggat
     3781 gagacttctg cagcatctcc agccacgttt ttggccatat ccgcgatgat ggtaaatgtt
     3841 gtaatgacct tgaatttttc agttgcactt gcctgaaatg cgatcgagca ggtgagggcg
     3901 aggcacccca agagcatggt tacttttttt attgagtgca taatttagta cctatagtga
     3961 gcaaaattag aacaaacata gcaacggcta tgttttatag cacaacctat acaaaatgaa
     4021 aatgattatc attaacaact ttggggccca aaacagggat taggttgttt cctgctggcg
     4081 acatggtatt gaggaaaaaa gcagggcagc tattaggctt tgaaatgact ttatgaggaa
     4141 aatttttgat ggcaaaaatg gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa
     4201 agagtggatt atcgtagctt attgttgtcg ctgataatgt ttaacatatt gaaaaataaa
     4261 taaaatactt atgagtcaga tagttgtgat ttttgccctt acttgttcag gttgtattgt
     4321 tctttcttac taatttcttg attttgcgac atttaaaagc gactcaattc gttatatggc
     4381 atcagaagag tatgcgtcat gccggaacgc ccagcataag aaatctgata taaaaactgt
     4441 ggcgtgtatg gtacggatta gaggggaaaa tgtcagcaca tttgcgaaat gaatcaaaaa
     4501 gcccgcagca atgtgcgggc gttagtgtca gcgcacaacc agcacggagc actctgcgtg
     4561 acgcacgaca gctgcggcgt tggaaccgag cagataagtg gtgatatccg gtcgatggga
     4621 agcaatgatg atcatatgag cggggatctt cttcgccaat tccagaatgc ggtctttggg
     4681 cgagccttcc tcaacatgga catgcactct gtcggttggc agtttaaatt ttttaatgat
     4741 ctcttccagt tgcgatttgg cttccgcttt caggtcatcc attgccggta attctgcgga
     4801 atacgctaaa cccagagagg catagtaggg cagtgaaggt attaccgtca ggaaatgaac
     4861 ctctgcatca tcaatctttg cctctgcctc aacgtggcta atcacgcgtt gagttaattc
     4921 tgaatcggaa atatcgatag ggacaagaat cgttctgttc ataaaacctc ctgttttagt
     4981 atccgcataa agtgtaacgc cagatgacac tttttgtgta atgacgaagt tcacattttt
     5041 aatttagatc aaaggaggaa gaataagcag aaaaagcccg ccataacagc gggcaggagg
     5101 atttagaact gataaaccag acctaaagcg acaatatcat cggtagagat gccgttggca
     5161 gcgtagaagc tgtcatcttc atccaacagg ttgattttat agtcaacgta ggtggacata
     5221 tttttgttga aatagtaagt cgcgccaaca tcggcgtatt taaccagatc tttatcatca
     5281 acacctgccg ggttgtctgc accaccggca gcgtgcaggt cacggccttt agacatcagg
     5341 aaagagactg ccggacgcag accaaaatca aactggtact gtgcagtaac ttcaaagttc
     5401 tgggttttgt tagcaacagc ataatcgctg tcgccaaacg gggtcatatt acgcgtttct
     5461 gaatacatgg ttgccaggta aatattgtta gcatcgtatt ttagcccagc agtccacgcg
     5521 tctgctttat caccacccgc cgcagtatgg ttaacctggt cattggtgcg gtcagaagag
     5581 gtgtatgccg caccagcgct aaagcccatg cctaaatcat atgttgtgga aagaccccag
     5641 ccgtcaccgt tttcatggcg aacatcacgt ccgttgttgg tgccttcctg accattactg
     5701 gcaccttcgt tgttaccttg atactgcacc gcgaagttca gaccatttac cagaccgaag
     5761 aaatcagtat tacgataagt cgcgacgcca ttggcacgac cagtcataaa gttgtctgca
     5821 ttggtataag agtcaccgcc aaattcaggc agcatatcgg tccagccttc gatgtcgtac
     5881 attacgccat aattacgtcc gtaatcgaaa gaaccgtaat ctgcaaattt caatccggca
     5941 aatgccagac gggtccatga ctggtttttt gaagattcag tgttgtttgc ctgaatattg
     6001 tattcccatt gaccgtagcc agtaagttga tcgttaattt gggtttcgcc tttaaaaccc
     6061 agacgcgcat agctctggtc gccatctttc gctgaattat cagaaaaata atgtagtcca
     6121 tcaactttgc catacagatc taatttgttg ccgtctttat tataaacttc ggctgcatgt
     6181 gcagcacctg cggcgagcag ggtaggaatt aaaaatgcca gtactttgct tttcattgaa
     6241 taaatccttt agttatttta tttgcctttt catccccatt ttggggagtg aaaacatcct
     6301 aaaggagaag ttccgttagc cgataaatat catttgttac gccttaagta aaacc
//
LOCUS       DARNVM010000222         6194 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.222, whole genome shotgun sequence.
ACCESSION   DARNVM010000222 DARNVM010000000
VERSION     DARNVM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.222"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            378..878
                     /gene="tnpA"
                     /locus_tag="SI621_004376"
                     /pseudo
     CDS             378..878
                     /gene="tnpA"
                     /locus_tag="SI621_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1072..2409)
                     /locus_tag="SI621_004377"
     CDS             complement(1072..2409)
                     /locus_tag="SI621_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4067013.1"
                     /translation="MVSGFAMPKIWRKLAMDILVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVAAAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVTSTLWFFLT
                     WFPNYLTQEKGITALKAGFMTSVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFVGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILMVGDVKRVG"
     gene            complement(2484..3386)
                     /gene="dgoD"
                     /locus_tag="SI621_004378"
                     /pseudo
     CDS             complement(2484..3386)
                     /gene="dgoD"
                     /locus_tag="SI621_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(3388..3606)
                     /locus_tag="SI621_004379"
                     /pseudo
     CDS             complement(3388..3606)
                     /locus_tag="SI621_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(3603..4292)
                     /gene="dgoR"
                     /locus_tag="SI621_004380"
     CDS             complement(3603..4292)
                     /gene="dgoR"
                     /locus_tag="SI621_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HEI4067014.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            complement(4680..4978)
                     /gene="tnpB"
                     /locus_tag="SI621_004381"
                     /pseudo
     CDS             complement(4680..4978)
                     /gene="tnpB"
                     /locus_tag="SI621_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            5489..6194
                     /locus_tag="SI621_004382"
                     /pseudo
     CDS             5489..6194
                     /locus_tag="SI621_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1645 a         1577 c         1514 g         1458 t
ORIGIN      
        1 ctcgcgtaac ggtatttaat gactacagca cgttccagga aaagcagtgg aagcaaatat
       61 tggtcattat gtcgaaaaca ccggaaacgt gctctatatt gtgtagtcat ttattcaaat
      121 gacagcgtat aaacaaattg atcagaatca catttttttg tgaaaatcat tgtcgagagg
      181 caacatttca tagtaagtgt atcctacagg tcatgtataa aacctacatc atgtaaacaa
      241 actcatcgaa ttgcacctcc tgtttgtgga ggtgcttttt tgtgcacagc tccccagcca
      301 ggctgggggt tccggaaagc tttcagtttt gagccagtta ttaaaacctc ttttgatttg
      361 ttaaaacacc ttgcggtatg gcaacagcaa gtgtcaaaca aaaaatcaaa agggggaccc
      421 aatggggaac gaaaagagct agcgcacccc gatggaactg taaatatcac atagtttttg
      481 cgccaaaata ccgaagacaa gtgttctaca gagagaagcg tagagcaaca ggcagtattt
      541 tgagaaagct gtgtgagtgg aaaagcgtac ggattcttga agctgagtgc tgtgcagatc
      601 atatccatat gcttgtggag atcccgccca aaatgagcgc atccggcttt atgggatatc
      661 tgaaagggaa aagcagtctg atgctttacg agcagtttgg tgatttgaaa ttcaaataca
      721 ggaacaggga gttctggtgc agtgggtact aagtcgatac ggtgggtaag aacacggcga
      781 agatacagga ttacataaag caccagcttg aagaggataa aatgggagag cagttatcga
      841 ttccctacct gggcagcgcg tttatggacc gtgagtaacg aagttggatg caaatgtcag
      901 atcgtgtgcg cctgttaggg cgcggctggt aagagagcct tataggcgca tttgaaaaac
      961 ctccggctat gccggaggat atttatttct tcccaccagg tgagatggtt actgaatatt
     1021 tctatctgtt cagcacaggt atgagaaaaa agggcttcct cagataagag attacccaac
     1081 gcgcttcaca tcgcccacca tcagaatgta agagagcgcg ccaatcaacg cgacggcgga
     1141 gatataaacc agtgcaggtg cgaaaccgta accctgcgcc aggtacccca ccaccagcgg
     1201 aacggtgatg ccgcccagac cgccaacaaa gttaaatacg ccaccggtta aaccaatcag
     1261 gcgcatcggt gccagagaag agaccagcga ccaggtaatc gaagcaaaac cgttgccgaa
     1321 gaatgccagc gccatcaggc acataatcat catcggatcg ttagtgtagt tagcgcccat
     1381 aatgcaggtg gagatcagca agccgcagat aatcggcgtt ttacgcgcaa agcccagtga
     1441 aaagccctta cgtaccagca gatccgcgac ccagccagag agcaggacgc cgacaaacgc
     1501 cgcgaggaat ggtaccgagg tcataaagcc tgctttcagc gccgtgattc ctttttcctg
     1561 ggttaaatag ttcgggaacc aggtcaaaaa aaaccacagt gtagaagtca ccgcaaattg
     1621 tccaagatag acgccgatca gtttacgatg gaacaccagt ttccagtctt tggctgttaa
     1681 cggctgacgt gcctcttttt tcaccggcgc atcaccatcc accagaccgc cgccatcacg
     1741 aatgtaatcc agttcagctt tgctgatgcc tttggtcagg cgcggcggct gataaacctt
     1801 aaaccaaatc agcgaccaga taatgccgat accaccggtg acaatgaaca cccagtgcca
     1861 gctcaacatc tcctgaatcc agatcagcag cggagtcaga aacgccagac cgacaaactg
     1921 accagacgta taaaaaccaa cggcagaagc gcgttcatgt tccgggaacc agctggtcac
     1981 catccggtta ttggtcggga aagcgggcgc ttcgaaaata ccggttatcg cgcgcagacc
     2041 aattaatgac attaatcccg tggcaaagcc ctggaataac gtcgccactg accagccaaa
     2101 tatcgcaata aaataagtca cgcgtgaacc tacgcgatct aaaaaccaac cgccggggat
     2161 ctgacatagc gtataaagcc aggcgaaggc cgaaaataca tagcccattt ccgctttggt
     2221 aatgccgaac tcttcctgaa tatgggcggc agccacggcc aggttagcgc ggtcaacata
     2281 acaaataacc accgtaataa agatcatcac cagcgtcaga taacgccgac gccccggttt
     2341 tgctgcatta acgagaatat ccatagcgag ctttctccag attttgggca tagcgaagcc
     2401 gctcaccatg ccctgtaatt tacagagggt tatatttttg tattgctgtc ttagtgcccg
     2461 atgaggggct tacgtggcag gaattaccac tctgctacgc tgttatcttc atgacgccag
     2521 agcggattac gccagtccgg ggcattttta ctgaactcaa taactttagc ttcgtcgatt
     2581 tccacgccta agcccggttt cgttagcggt ttaaagaagc cgccgaccat gctgaagtcc
     2641 tctttgtttt tcacaaagtc gagtaactcc gcgcctttgt tgtaatgaat gcccatactt
     2701 tgttcctgaa gtacggcgtt ataggaaaca aagtcgatat gcaggcaagc tgccagtgca
     2761 atcggtccga gcggacagtg cggcgcaagg gtcacgtcat aggcttctgc cattccggca
     2821 attttgtagc attcggtaat accgcctgcg tgggagagat ccggttgcag aatcgaaata
     2881 ccacccgcct ccagcacacg tttaaaatcg aaacgtgaga acatacgctc acccgccgcc
     2941 agtggaatat gcgtttgtgc cgccagtttc gggtagtatt cggcctgttc tgccagcacc
     3001 ggctcttcaa taaacagcgg gcgatacggt tccagttctt taatcagtac tttcgccatc
     3061 ggcgcgctga cgcgaccgtg gaaatcaaga ccaaactcaa tctgattgcc aaaagcttca
     3121 cgaatttgtg ccacggtgtt gaccgccgca tctaccgcgc gggagttatc aattagcccc
     3181 agttcttcac aaccattcag tttgaaggta tcaaaaccga tttcgcgcag gatttttatt
     3241 ccgtcgataa cattcgccgg acgatcgccg ccgacccaac tgtaggcttt aattttgtca
     3301 cgcaccaggc cgcccatcag ttgccagacc ggcgaattca gcactttgcc tttgatatcc
     3361 cataacgcct ggtcgatccc ggcaattcca gccgacattg ctgccgacca ttcctgccat
     3421 caccactggc gtattttctt cacgccagtc ggccgtcact tctgctaaca ccgcagccgg
     3481 agatttttcg ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca ggcagtggtc
     3541 gccctgataa agccaggcgc gcagattggt cgatccccag tcaattgcga tgtagcgagc
     3601 tgtcatgtga tttcctttaa ccttcgtgtc gagctggcga tcatggtaag cgccgcctgc
     3661 tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg ttcctggagc
     3721 gtttgcggca tgttggcctc atcacccatc caggttcgtt caaataccgc ccgctgcagc
     3781 gaactgatcg caatgctaag ttgctgtaac accggattat gtaccgactg cagcaccgcc
     3841 tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt ggcaatcatc
     3901 tcgttcagcg ccgattcaat ctgcgccaga tcgctggaag tcgcgcgctc tgctgcccaa
     3961 cgggcaatcg ccggttccac cagatttcgc acttcgctca tggcactgat aagtcgtggg
     4021 tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt cgaggtaatt ccactggtta
     4081 cgcggtgcca caaacgcgcc gcgataacgt ttcatttcaa tcagccgctt cgccatcagc
     4141 gaacggaaca cctcacggat gatgttgcgc gaggttgcaa actcctcaca gagctccgcc
     4201 tcagccggaa gcggcgagcc tggcacgtat ttgccgtgaa cgatctgttt acccagcgta
     4261 atgacgatgc gatcggtttt attgagagtc atggagagtc cttgtgctct gtatgttctt
     4321 ctctacttta ccccgatcga tgcataacgc ggcaactttg tagtaccagc gtgataacgt
     4381 tcgcgtttgc cgtgtgtgta atgtagtaca aacttatatt gttgtactac aatttagatc
     4441 ataaaaagaa caatgcataa aaaaatgaca tgcgtcgggc agaaatctga aaagggatgt
     4501 caggcgctaa acaggaggga aagaagagta tgctttcaac gacttagtac tcagacagca
     4561 gcttttccag cgtcagcgat gcgaatgagg tagttctgat tcatgaccgt ttttataatc
     4621 cggctatgat atttttacca cattgtcagc gcgttaaggc gtgatgtttt tggctgacgc
     4681 tagcccagct tactaaggag cattgccagc tgcgggcggg taaaggatac cccgccgtca
     4741 cgtaccgcaa gccagataaa ttgccttcct cctggcgctt ggtgaaaaga cacagaccat
     4801 cagcccagag tattttaaca gtgtcacccc gtcggcctcg gaagataaac aggtggccga
     4861 agaagggatt ataattcagc acatgttgac cctgttttcc cagtccgttg aaggatttac
     4921 gcatatcagc taccccggca atgaggcaga tacgggtacc ggatggtagt gagatcatcg
     4981 tctcctcctg gctaactcac ggattaacac cttgagcagc tctggtgaag gattttccaa
     5041 cacatcatgt tactgagacg gaactcaacc ttacaggaac tgacactgac tgtgttatgg
     5101 ggggaaatgg ataaagatgg agcaatagac atcatcgtat ttttctgttc attcggcgtt
     5161 atctcaacag gtaataattt aacgccagcg tcagaagtag tcgttacagg aagacgccgc
     5221 gaaacaacac aggttcagga gaataaggta gagcgtctat agtcctgttt cctccatcta
     5281 ggatgtctga tggaaattca tcagatacac taaggatccc cataaagtat tttattggca
     5341 cacatcagac atcctttagg tatccagcaa gttattgaac tattgcgtcg tctcgttcaa
     5401 acttctcttt tattgcctca ataatatatg agctaaaatc cagagatgtt tttgtagtgg
     5461 cacagtaaat ttggccacct gattaaaggt gatatcctca ccacaacaca aaacaggtga
     5521 cttaatgaac aagaaaacca aacgtacttt cacccctgaa ttcaggctgg aatgtgcaca
     5581 gctaattgtt gataagggct actcatatcg acaagccagt gaagcgatga atgtcggttc
     5641 taccacgctt gagagttggg tgcgccagct caggcgagag cgtcagggga ttgcgccctt
     5701 tgccacacct attactccag accagcaacg tatccgcgaa ctggaaaagc aggttcgccg
     5761 cctggaggaa cacaatacga tattaaaaaa ggctaccgcg ctcttgatgt ccgactcgct
     5821 gaacggttca cgatagttgc cagactgagt gacagccact cagttgtcag cctctgttct
     5881 gctctggaaa tacaccgcag tagttaccgg tactggcgaa aacgacgcga tacggttaat
     5941 ccggcgcgag tcaggttgtg cagcgaaata cgccgggcgt ggaaccaaag taggggctct
     6001 gcgggggcgc gcacgctggc tgaaatgctg acccaaaacg gcgtcccgat gagccgttac
     6061 cgtgccgggc gtctgatgaa atatctgaac ctgagcagtt gtcagcccgg aaaacatcag
     6121 tacaaaaatg ctcgtcagga acatacctgc ctgccgaatc tgcttgagcg ccagttcgct
     6181 gtgcccgagc caga
//
LOCUS       DARNVM010000223         6105 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.223, whole genome shotgun sequence.
ACCESSION   DARNVM010000223 DARNVM010000000
VERSION     DARNVM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.223"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(184..1575)
                     /gene="uhpT"
                     /locus_tag="SI621_004383"
     CDS             complement(184..1575)
                     /gene="uhpT"
                     /locus_tag="SI621_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HEI4067015.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(1831..3150)
                     /gene="uhpC"
                     /locus_tag="SI621_004384"
     CDS             complement(1831..3150)
                     /gene="uhpC"
                     /locus_tag="SI621_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HEI4067016.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(3160..4662)
                     /gene="uhpB"
                     /locus_tag="SI621_004385"
     CDS             complement(3160..4662)
                     /gene="uhpB"
                     /locus_tag="SI621_004385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HEI4067017.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNIWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHSVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(4662..5252)
                     /gene="uhpA"
                     /locus_tag="SI621_004386"
     CDS             complement(4662..5252)
                     /gene="uhpA"
                     /locus_tag="SI621_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HEI4067018.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(5414..>6105)
                     /locus_tag="SI621_004387"
     CDS             complement(5414..>6105)
                     /locus_tag="SI621_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067019.1"
                     /translation="DASRHVGEASSFIACCGVKINSTIHELLRSKTMALSGHGGVRKY
                     KDFEKLFSEIVFTPDECNIVRNSLQKDGVEKKTRENIISCLSSISGIGNLYYACSQAF
                     PVALDRGDINAIQASNSISAGFNSNPGFCALMGKTNFLFADFSQHTFTQTHYIVKAEI
                     NEAGNDFNIANFIYRTYGEDYIHNDSVDDSLNERSAEDYRFLDDSLLPENDFRNRRRN
                     FSGNIAIATYK"
BASE COUNT         1633 a         1728 c         1472 g         1272 t
ORIGIN      
        1 accccctttt gatttcttgt ttgacacttg cagttgccag accgcaaggt gttttaacaa
       61 atcaaaaggg gttttaataa ctggcttaaa gctgaaagct ttccggaacc cccagcctag
      121 ctgggggttt tctgtgcaca aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta
      181 cgtttatgcc actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat
      241 caccgccact atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcagcgaa
      301 ggtgcctgcc cagccggtaa ggccgaatac tggcgtccca tcggcaatca ttcccagacc
      361 taacttggca aagctgtcgc caatcaggta agcaaaggtg cctttaatac catcggcagc
      421 gccaatcgct tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc
      481 aaagaccagg aaacccaacg caaagagaga agccaggtag atatattcgt tgctggcgtg
      541 ttgatacaca ccgagcgtgg cgataatcag cgccagcgcg atacaggcca ccaggccacg
      601 gcgaccgttc gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcgccagc
      661 ttcaaacagc gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc
      721 gtacacagtt gaccactggt cgataccaat acgcaccaca tagaggaaaa tgttggcgaa
      781 gcacagcagc cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt
      841 catatcggta gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagctcttc
      901 agctttgccg aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc
      961 gacaatcagc gcgataatcg acgggaaaat aaacatgccg atgacatgac catcgaacag
     1021 gtaatttgcg ccgaacagcg ccacacccgc tgcgcctgca ccgccaaggt tgtgagaaat
     1081 gttccagaaa ccgaggaatg tcccgcgttt acgacgcggc gtccatttcg tgatggtgga
     1141 gtaactgcac gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat
     1201 caggaacagg ctaaccgagc cgctgcccat actggcgctg aagcccagca tacaaatggc
     1261 agacaggatc agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga
     1321 aaccagcgtt ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg
     1381 cgtcatgctc aacccgtagg tcgagatcat gtcgttctgc gcgatgttaa agttcttgcg
     1441 aatcaggtac atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt
     1501 gaaccacatt ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt
     1561 taagaaagcc agcatgggtt actcctgatt tcccttcata cttcaacccg caggtgcctt
     1621 agctgcacgc attcctcccg gtcacatagt tcactatgct cccgggactc acgcgtttgc
     1681 tgcgttcctg caacttgaat tattttggga aagaattaat gaatgatggc attgtaaaaa
     1741 tcagcagcaa atcaggcctg agatagcgtc caggcaaaac ctggaaaatt tcttagtttt
     1801 gccggatatg aagtgaaaag gtgagatgca tcacgcttct cgcggtgtct gggcgttcaa
     1861 aaagggcagt aacagcagtg cggaaatccc ggcggcaatg gcgataacta caaaaaatcc
     1921 gctccagtgc caggtatcga gcactttcgc cagcggccaa ccagcaagcg acgcccccag
     1981 ataagcaaac aagccgacaa accccgtcgc cgcccctgcc gcctctttgt gggaacattc
     2041 tgccgccgcc atgccgatta acatctgcgg gccaaagaca aaaaaaccaa tggtgaagaa
     2101 gcaagttgcc tgcatcacgt agctggcaaa tggcatcagc cacagcgaac cgactgaaag
     2161 caaaattccg gcggcgaaaa tcaaattcat cggcccgcgg ttgccgttaa acagtttgtc
     2221 cgagccccaa cccgctacca gcgcaccgat aaatccgccc agttcaaaca tcgtcactgc
     2281 cgtattcgcc gtgaccagat cgacgcccag cgtctcggac atatacaaat tgccccagtc
     2341 gttgattgcc gcccggacca catagaccag cacatagcaa aacgaaagca gccagatata
     2401 cggattcaac aacacatact tggtgaggat ctctttacgc gtcagccctg ccccttcttg
     2461 ttgttgagca atctccagcg catcgtgccg ccattcacca accgcaggta aacctaacgc
     2521 ctgcgggcga tcgcgcagcc gccagcagag aaaaatcccc acgacgatcg ccatacaacc
     2581 agcaatcatc atcccggcac gccagccgta atgcagcgca gctgctgcca tcacaatggg
     2641 aatgagcgca ccaccaacgt tatgcgccgt gttccataat gcccaccaac caccgcgctc
     2701 ggtacgagaa taccaggccg ttaacagacg tgcacacacc ggtgaacccc agccctggaa
     2761 aaaggcgttc agcacccaga gtacggcaaa cgcccatagc gacgtcgaga agccaaacag
     2821 aatgttgatg atgcccgtgg cgataagccc tatccccata aaataacggg catttgagcg
     2881 gtcgctgaca atgccggaga caaacttcga cacgccgtag gtaatgtaaa acagggtcgc
     2941 taacaggccg atatcgctac ggctgagcac gccattagca aggatttctg gtacggcggc
     3001 gttaaaactt ttccgcgtga agtaaaacag cgcgtaaccc agccagatgg tcagcagaat
     3061 atgccgacgc cagtagcgat aacgggcatc aatttcatgt ttatcagtca ttaatggcgc
     3121 atcggcaggc gctttcagaa acggcaacat catcaaacct taaacataac gttgaggtag
     3181 agaaacgctg acacgcgtgc cgtgcagaca ggaaatggtc aaggtgccgc ccagcgccgt
     3241 tacgcgctcg cgcattccgg tgaggccaaa accttgttgc ccggaacccg gcggcaaacc
     3301 gctgccatcg tcttcaatca ccagcatcaa ccgttcatcc tgctgccagc cttgcagggt
     3361 gaccgcgctg gcatcggcat gtttcacaat gttgttcagc ccttcctggc agacgcgaaa
     3421 cagcgtcact cgctggtttt cacttaacgc tgattcatcg attcgccatt cgagatggct
     3481 gacaatgccg cgcccttcca gctccatttc ccgcatcagt gagcggatgg cctgctccag
     3541 ggtgagatca tccaactggc gcggacgtaa ccgacctaac aaacggcgca ccgcatcgta
     3601 aacgcccagc gacagttgtt cgatgagctg cccgctctgc ttcacactgg cgttatctgc
     3661 cgcaagtcgc tggacaatgc ccgcttgagt acgaatagca gtgatggtct gaccgatatc
     3721 atcgtgcagc tcacgcgcca cgtcgcggcg cacgctctct tcagtttcta acaaacgttc
     3781 agccagatgc tgattgcgcg ccagttcctt ttgcagcgac tggttaagtt cacgcaaccg
     3841 ctggatgcct gcgcccagta acaaccctgt cagactttgc accagcagcg agagcaataa
     3901 atccaccgaa tgatcgcgcc aggtttgact ggcgatcagc gcgatggcgt tcatcaacgt
     3961 cgcaatcagc gccccttgcc agccatagtg ccaggccagc gcgataatcg gcagcgccag
     4021 gcagaatggc gtaaagcgcg acagttcgtc cggcaatccc aactggagcc agagactaat
     4081 aacaaacagc agcaagtacc agaccagatg ccgcccgcgc cagttgatcg gttgagaaac
     4141 taacgacggc ccgagcggca gccagatgtt attggcgaga tagtgccaga acaccagaca
     4201 tatcggggcc agcgtcaagc cgccagttaa agtcagcaac agcgcattcc acgactcttt
     4261 gccgtgccaa agccagggca gcgactgcaa caacgccgcc gccgtcagcg ccgcaccctg
     4321 taacagcaag gtgcgccagt cacgttgatg gcgatagcgc gagatcagcg ccacgggcaa
     4381 taacgtcagt aaactaccga tcatcaataa ggagaaatgg gttaaaccga ccgcctgcgt
     4441 tagccagtaa atcagcagcc attccgcgcc cagcaatacg ggccagtatc cgcgcgggca
     4501 ttgcagcatt agccccagac gcagaccaaa cggaaataac agcaccgcca tatcagggcg
     4561 ctcaaccagg tgcaggctga tactccacag gcaaaaccat gcggcagaga agataaaaaa
     4621 gcaggcaata acggtaatta agcgggagaa caacgtcttc atcaccagcc atcaaacatg
     4681 cggcgcgcca gttcaacgtc gttactgacg cccagttttt ccatcagatt ggcgcgatgg
     4741 acgtgtaccg ttttcggtga caagcccagt tcggcggcaa tctctttcac cgccattcct
     4801 tgcgccagtt tttccgccac ctggcgttca cgtttggtta gcgggtcctg gcgaccggat
     4861 gccagtttaa tggcaatatc cggcgtcaga taacagccgc ccgtggcaac cgtatgcacc
     4921 gcagcaatca gttcatccgg gctacagcgt ttggagagaa agccgcgcgc ccccgcgtta
     4981 agcgcctgct caaccagcgc cggactgtca tgaacggaga gcataatcgt cgccatacct
     5041 ttcggcagct ggcttagcag ctccagaccg gagatatcgg gcatggagat atcgcaaata
     5101 cacacctgca caccgcgccc cggcagcccc gccagcgcct cgcgacccga accaaactcg
     5161 gcaactactt gcaaatcagg ttccagcccc agcaactgcg caaagccgga gcggacgatg
     5221 aggtgatcgt ctataagtgc aacggtgatc atgattgggt cctggcggat aaaaaacgcg
     5281 cataccttaa cgataagcgc gatgttgttc aagttggata tggggtatgt attggcctaa
     5341 tgatgttggg cttatcgtca aaacgcttac ctgaaaggta agcgtttagt gcgtgttatt
     5401 gagaaaaagc gaattatttg taggttgcga ttgcaatatt gcctgagaaa tttctcctcc
     5461 tgttgcggaa atcgttttca ggtaacaaag agtcatccag aaacctataa tcttcagcgg
     5521 aacgctcatt aagagagtca tccactgagt cgttatgaat ataatcctca ccgtatgttc
     5581 gataaatgaa attagcaata ttaaaatcgt taccagcttc atttatttcg gcttttacaa
     5641 tataatgagt ttgtgtgaat gtatgctgtg aaaagtcagc aaaaagaaaa tttgtttttc
     5701 ccatcaaagc acaaaaccct gggttactat taaacccggc agaaatagaa ttgcttgcct
     5761 gtattgcatt aatatccccc ctatcaagcg cgacaggaaa tgcctgtgaa caagcataat
     5821 acaggttacc tattcctgaa atactggaga ggcaagaaat aatattttct ctggtttttt
     5881 tctcaacacc atctttctgt agagagtttc tcacaatatt acattcatct ggcgtaaata
     5941 caatttcact aaaaagtttt tcgaaatctt tatattttct aacacctcca tgaccagaca
     6001 aagccatggt tttactcctc agtaattcat ggatagtgct atttatttta actccacaac
     6061 atgcaataaa tgagctagcc tctccaacat gtcttgatgc atcag
//
LOCUS       DARNVM010000224         6018 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.224, whole genome shotgun sequence.
ACCESSION   DARNVM010000224 DARNVM010000000
VERSION     DARNVM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.224"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..101)
                     /locus_tag="SI621_004388"
     CDS             complement(<1..101)
                     /locus_tag="SI621_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEI4067020.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNA"
     gene            379..2811
                     /locus_tag="SI621_004389"
     CDS             379..2811
                     /locus_tag="SI621_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEI4067021.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKHNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDIDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2878..4347
                     /locus_tag="SI621_004390"
     CDS             2878..4347
                     /locus_tag="SI621_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEI4067022.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4347..>6018
                     /locus_tag="SI621_004391"
     CDS             4347..>6018
                     /locus_tag="SI621_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEI4067023.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     QGWEWVTLATVGEIVGGGTPKSDNPQFWAKNGIKWITPADLYGLKGKYITSGARDISP
                     AGLSNSSARLMPKGSVLFSSRAPIGYVAIADAELSTNQGFKSCVPYIKESAEYIYYFL
                     LASAKKIDAEASGTTFKEVSGAIVSKILLPLPPLSEQLKIVSRANELMSLCDQLEQQS
                     LTSLDAHQQLVETLLGTLTDSQNAEELAENWTRISEHFDTLFTTEASVDALKQTILQL
                     AVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKSLPPISDEEKPFELPEGW
                     EWSYLSDIGILARGRSKHRPRNDPTLYADGTIPLVQTGDVARSNGCINTYSALYNQLG
                     LSQSKLWNKGTLCITIAANIADSGILNFDACFPDSVVGFTPYENEIPVLYFHYFMMTI
                     KSTLEKFAPSTAQKNINIDILSQLFFPCPPLEEFHRIVDKVQNLLSVCD"
BASE COUNT         1798 a         1431 c         1400 g         1389 t
ORIGIN      
        1 gcattatcta ccgtccaact gtaaggacgc tgataagaag gaataataaa ctttttgttt
       61 tcagtaagga tatcctgcac cttacattct ttagcttcca tttcatatac ccaatcaaac
      121 aatcaacaat aacattatct ttcttgctcg aaagaaacgc aactataaga tttttatata
      181 ttttgttaga gttgatatgg ttcctgtaac ttctgtttat ctgtctctga tatgcccaga
      241 ttaattcaag caagatataa cagagatatc aatgtgatct ctgctacgta ctttacgttg
      301 aaacaccgcc ctaaatcata gccaggctat tgattttatg tacaatcaga atcgtcctga
      361 taaaagcgag aataccccat ggcggaactg aacctaagta acctgacgga agcagacatc
      421 attaccaaat gcgttatgcc agccattctc aatgcaggct gggacaacac aacgcaaatc
      481 agacaggagg tcaaactccg ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc
      541 agaacggtaa aatctgctga catcgtgctg tatcacaaac caggcattcc cttagccgtg
      601 attgaagcaa aagccaacaa acatgaaatc ggcaaaggga tgcaacaggg cattgaatat
      661 gcgcgcctgc tggacgttcc ttttgttttt gccaccaacg gtgatggctt tatcttccgc
      721 gacgccaccg cagccgaagg tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc
      781 tcccccgctg aactctggca aaaattctgc ctctggaaag gatacacaca agctcagctt
      841 ccggtgatta ctcaggacta ttacgacgat ggcagcggta aatcaccacg ttattaccag
      901 cttcaggcaa tcaacaaaac cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg
      961 gtcatggcga ccggaacagg gaaaacctat accgcattcc agatcatctg gcgcctgtgg
     1021 aaatcaaaaa ataaaaaacg cattttgttc cttgccgatc gcaacattct ggtcgaccaa
     1081 accaaacata atgatttcca gccatttggt acggcaatga ccaaagtcag cggacgcacc
     1141 attgatcccg cttatgaaat tcacctcgcg ctctatcagg ctataactgg cccggaggaa
     1201 gaccaaaaag cgtttaaaca agtcgcacca gatttcttcg atctgatcgt gatcgacgaa
     1261 tgccatcgcg gcagcgcatc cgaagacagc gcctggcgag agatccttga ttatttcagt
     1321 tccgccaccc aaattggctt aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg
     1381 gattacttcg gcgatccggt ttacgtctac tcactaaaag aagggatcga agacggcttc
     1441 ctcgcccctt ataaagttgt ccgtgttgat attgatatag acctgcaagg ctggcgccca
     1501 accaaagggc aaactgactt aaacggcgaa gtgatcgacg atcgtatcta taaccagaaa
     1561 gatttcgatc gcacgatggt aatcgacgaa cgcacagaac tggttgccag aaccattacc
     1621 gactacctca agcgcaccaa cccgatggat aaaaccatcg tcttctgtaa cgacatcgat
     1681 catgcagaac gtatgcgccg cgccctggtt aatctcaacc cggagcaggt gaaaaagaac
     1741 gacaaatacg tcatgaaaat caccggcgat gatgaaattg gcaaagctca gttggataac
     1801 ttcatcaacc cgaaaaaacc gtacccggtt atcgcgacca cttcagagct gatgaccacc
     1861 ggtgtggatg ctaaaacctg caaactggta gtactggacc agaacatcca gtcgatgacc
     1921 aaattcaagc aaattatcgg tcgtggtaca cgcatcgacg aacgttacgg caaactctgg
     1981 tttaccatcc tcgactttaa aaaagccacc gaactgtttg ccgatgagcg tttcgatggc
     2041 attcccgaaa aagtcatgga taccacgcca gaggatatcg ccgacccaga atctgatttt
     2101 gaagagaaac tcgaagaaat cagcgaacat gacgacgaac aggtaacagg cgttgatgaa
     2161 ccgcctgcgc caccatacca ggttaccgat accgatgatg tcggcccact tccggaagaa
     2221 gacgagaaga aaatccgcaa gtttcacgtc aacggtgtag cggtgggcgt tattgcgcag
     2281 cgtgttcagt attacgatgc cgacggtaaa ctggttaccg aatcttttaa agattacacc
     2341 cgcaaaacac tgctcaaaga atatgcctcg ctggatgact ttacccgcaa gtggcaggac
     2401 gccgatcgca aagaagcgat cattcacgag ctggaacaac aggggatcat ctgggaagta
     2461 ctggcagaag aagtcggtaa agatctcgac ccgttcgaca tgctttgcca cgtagtgtat
     2521 ggtcagccgc cgttaacccg caaagagcgc gccgagaacg tgcgcaagcg gaactacttc
     2581 acaaaatact ctgaagcagc gcaagccgtg ctcgataatc tgctggataa atacgccgat
     2641 gcgggcgtac aggagatcga aagtattcag gtgctgaaac ttaagccatt cgacagcatg
     2701 ggcaccttac cggagattat taaaaccgga tttggcgacc gtaacgggta taatcaggcg
     2761 ctcagcgagc tggaaaacga aatctaccaa ttaccgcccc gctctgctta acaactctct
     2821 tttgcgcctt ctgcaaccat catgttgcag aaggccttgt ttttcatgga atacaacatg
     2881 tctatcagct cagtaatcaa atcattacaa gatattatgc gcaaagatgc cggtgtggac
     2941 ggcgatgcgc agcgtctcgg tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc
     3001 caggaagagg cgctggaact ggagcaggat aactatcaat atccgatccc acagcgttat
     3061 ttatggcgca gttgggccgc aaacgctcag ggcattaccg gtgattccct gctggaattc
     3121 gttaatgatg atctgttccc ggcgttgaaa aacctcactg cgcctatcga taaaaaccca
     3181 cgcggctacg tggtaaagca ggcgttcagc gatgcctata actacatgaa aaacggtacg
     3241 ctactgcgtc aggtgatcaa caagctgaac gaaattgact ttaccagcgc cagcgaacgg
     3301 catctgtttg gtgatattta cgagcagatc cttaaagatc tgcaatctgc gggcaatgcg
     3361 ggcgaattct atactccacg cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa
     3421 ctcggcgaat ccattatgga cccggcctgc ggtacgggcg gttttcttgc ctgcgcattt
     3481 gatcatgtaa agaacaaata cgtgaagagc gtcgccgatc atcagacgct gcaacaacag
     3541 atccacggtg ttgagaaaaa acagcttccg cacctgctgg cgaccaccaa tatgctgcta
     3601 cacggcattg aagtgccagt gcaaattcgt cacgacaaca ctctgaacaa accgctttcc
     3661 tcctgggatg agcaactgga tgtcattgtt accaacccgc cgtttggtgg cacggaagaa
     3721 gacggtattg agaagaactt tccagcagag atgcaaaccc gcgaaacggc ggatttgttc
     3781 ctgcaactga ttgtggaagt actggcgaaa aatggtcgcg cggcggtggt attgccggat
     3841 ggcacactat ttggcgaagg cgttaaaacc aaaatcaaaa agctacttac cgaagagtgc
     3901 aacctgcata ccatcgtgcg cttaccgaat ggtgtgttta acccctatac cggcattaaa
     3961 accaacctgt tgttctttac caaaggtcag ccaaccaaag agatttggtt ctatgagcat
     4021 ccgtatccgg cgggcgtgaa aaattacagt aaaactaagc cgatgaagtt tgaagagttt
     4081 caggcggaga ttgactggtg gggtaacgag gccgatggtt ttgccagccg cgtcgagaat
     4141 gagcaggcgt ggaaagtcag cattgatgac gtgattgcgc gcaacttcaa tctggatatt
     4201 aaaaacccac atcaggcgga aaccgttagc catgatccgg acgaactgtt agcgcagtat
     4261 gcaaaacagc aggcggagat ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct
     4321 gcgctgtctg gcaaggaggt taactaatga gtgtggaaaa gctgatcgtt gatcatatgg
     4381 aaacctggac ctcggcgttg caaacccgtt ccaccgccgg gcgcggcagt tccggaaaaa
     4441 ttgatttata tggcattaag aaattacgtg agctgattct ggaactggct gtgcgcggta
     4501 aactggtgcc gcaggacccg aacgatgaac cggcgtcgga gctgctgaag cgtattgcgg
     4561 cagaaaaagc ggaactggtg aaacagggaa aaattaaaaa acaaaaacca ctgccggaaa
     4621 ttagcgagga agagaagccg tttgaattgc cgcagggatg ggagtgggtc acactggcaa
     4681 ccgtaggtga aatagtcgga ggaggaactc caaagtccga taatccgcaa ttttgggcca
     4741 aaaatggtat taagtggatt actccagcag acctctacgg actcaaaggg aagtacatta
     4801 catctggagc ccgtgatatt tcacccgcag gactgtcaaa ctcatcagcc cggttgatgc
     4861 caaaggggag tgtgcttttc tctagccgag caccaattgg atatgtcgct atagcagatg
     4921 cagaactgtc aacaaaccag ggttttaaat cttgtgttcc atatatcaaa gaaagtgcag
     4981 aatatatata ttactttcta ctggcatctg caaaaaaaat agatgcagag gcaagtggga
     5041 ctacatttaa agaagtctct ggggcaattg tttcgaaaat attattacct ttaccgccat
     5101 tgagtgaaca actaaaaatc gtctcaaggg caaatgaact tatgtcccta tgcgaccaac
     5161 tggaacagca atccctgaca agtctggacg cacatcagca actggttgaa accctgttgg
     5221 gaacacttac agacagccaa aacgccgagg aactggctga aaactggacg cgtattagcg
     5281 agcatttcga cacactattt accaccgaag ccagcgtgga tgcgttaaaa cagaccattc
     5341 tgcaactggc cgtaatgggt aaacttgtgc cgcaggatcc gaatgacgaa ccagcctccg
     5401 aactgctcaa acgtattgcg caggaaaaag ctcaactggt gaaagaaggg aaaataaaaa
     5461 aacaaaaatc gttgccaccg attagcgatg aggaaaaacc gtttgaactt ccggaagggt
     5521 gggagtggag ttacctatca gatattggca tacttgctag aggaagatct aaacatagac
     5581 ctcgtaacga tcccactctt tatgctgatg gcacaatccc attagtacaa acaggtgatg
     5641 ttgcaagatc taatggatgt ataaatacat attccgcgct atataatcaa ctaggattaa
     5701 gtcaaagtaa attatggaat aaaggtactc tttgtattac gattgctgca aatattgcag
     5761 actctggtat tttaaacttt gatgcttgtt ttcccgatag tgtagttggc tttactccat
     5821 atgaaaatga aatacctgtt ctttattttc attatttcat gatgacaata aaatccactc
     5881 tggaaaaatt cgccccatca actgcgcaaa aaaacatcaa cattgatata ttaagtcaat
     5941 tgtttttccc atgtccacca ttagaagaat tccatcgtat tgttgataaa gttcaaaact
     6001 tattatcagt ttgcgatg
//
LOCUS       DARNVM010000225         5990 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.225, whole genome shotgun sequence.
ACCESSION   DARNVM010000225 DARNVM010000000
VERSION     DARNVM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.225"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..69)
                     /locus_tag="SI621_004392"
     CDS             complement(<1..69)
                     /locus_tag="SI621_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase"
                     /protein_id="HEI4067024.1"
                     /translation="MNFYKRTALAVLVMGFSGAALAL"
     gene            complement(558..1883)
                     /locus_tag="SI621_004393"
     CDS             complement(558..1883)
                     /locus_tag="SI621_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HEI4067025.1"
                     /translation="MTVLQIVVLLGAIFLGVRMGGIGIGYAGGIGVLILGLCLDMKPG
                     NIPWDVILIIASVISAISAMQLAGGLDYLVQVAERILRKNPKYINYLAPVVTYVLTIF
                     AGTGHTAFSMIPVIVEVSKEQNIKPSCPLSIAVVSSQIAITASPVSAAVIYMSGVLEG
                     FGWSYPVLLGIWLFTTFVGCMLTAFIISLISDMKLDNDPVYRERLSKGLVSAPVKSVN
                     KQLKPYARRSVAIFLIGVILVVLYASAISPTLGLIDNVVVSRDAAIMSLMLLVGGFIT
                     LFCKADINKIADSSVFKSGMVACICVLGVAWLGDTFVSGHSGEIKELARTTVSQYPAL
                     LAVVFFLAAMLLYSQAATAKAITPAIVTALGITAANPDDSYMLVASFAAVSALFVLPT
                     YPTLLGAVQMDDTGTTRIGKYVFNHAFFIPGVLAIVFSVLLGFLVVSMF"
     gene            complement(2048..3448)
                     /locus_tag="SI621_004394"
     CDS             complement(2048..3448)
                     /locus_tag="SI621_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEI4067026.1"
                     /translation="MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFF
                     IIAFGYVKKAAAMANKELGTIPADVADALIWACDQLIDGKYREQFVTDWLQGGAGTST
                     NMNCNEVICNLAAEKLGGVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLL
                     GAVEHLVGAFYKKADEFSRVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQ
                     EHLRVVNLGATAIGTCVTAHPDYPALAVKYLAQITGINFRNSEDLIAATSDCGAYVAL
                     SSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIMPGKVNPVIPEVTNQ
                     SCFLVQGLDTTVMLAASAGQLELNVMEPVITFALFTSLKVMTNACNTLRTKCIDGITA
                     NSDRTAEMVMHSCGIVTLLKPHLGYKVCSEMAHEAYHTGKSLHQIVVVERKLLTQEEW
                     EKTFNLDNLIAPKFEQ"
     gene            complement(3671..4969)
                     /locus_tag="SI621_004395"
     CDS             complement(3671..4969)
                     /locus_tag="SI621_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HEI4067027.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNFMRRNPKYINIIAPIITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSI
                     KPGATLSVGLFMLATIAIVILAAFPQLRPGFDISKPMETRDIIIICMLSAACLMVILC
                     KMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQTYPMLLAL
                     VLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV"
     gene            complement(5028..5747)
                     /locus_tag="SI621_004396"
     CDS             complement(5028..5747)
                     /locus_tag="SI621_004396"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEI4067028.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQRDQEHIPLIISSIP
                     DIPDRTDALMHNGLSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIIDTTVDEVRVCGKTRIGLLATDATLYMGLYQKGIESLGFNYITPDADGQKNVMKSI
                     YSLKAGNVTHAQKLMEEQAENLFSRGAEILILGCTEIPIILSQAVKDQPLRYIDSTAS
                     LVRAGIKWYENRIGKDQHLTQ"
BASE COUNT         1858 a         1366 c         1221 g         1545 t
ORIGIN      
        1 taatgccagt gctgcaccac taaaacccat aaccagtacg gcaagtgccg ttcttttata
       61 gaaattcata tctacctcct ggactgtgaa atatatttac cttgtatttt tatacagaac
      121 agttaatgcc ttataaattt gcttaataat gatataatta taataaatcc attctcttta
      181 acttgtcatc aactcatcca gaataagatg ataaacagtt atatttcttg atataatatc
      241 cctccttgac aggtgggcga acaacgttaa ctatcggtaa acagacaccg cattccgtga
      301 taacatcatt catctaaaat attcttgtcg atgtagagaa catcaatgta aatagaatgt
      361 ttaattacct ctcattcaga atagcaatga gcgtccatca tgttgtgtga ttagaatcgt
      421 ataataaatg ttgcactaaa atatttataa cgaagtagca atacgcacct caatactaat
      481 agattgaatg ggctaccaat caatgtggat acgtattgac gggtagccag gagatagaaa
      541 ctacgttcgg tcaagaatca gaacatactc accacaagaa atcccagaag cactgagaaa
      601 acaatagcca gcacacccgg aataaagaaa gcatggttga acacatactt accaatacgg
      661 gtcgttcctg tgtcatccat ttgcaccgcc cccagaaggg ttgggtaagt tggtaacaca
      721 aataaagcag acacagcagc aaaagaagct accagcatgt aactgtcatc cggattcgct
      781 gcagtaatac ccaacgcagt cacaatagcc ggagtgatag ctttggcagt tgcagcctgt
      841 gaatacagaa gcatcgcagc caggaaaaat accacagcca gaagagctgg atactgggat
      901 acagtagttc tggcaagctc cttaatttct cctgaatgac cagatacaaa agtgtccccc
      961 aaccatgcca cacccagtac acagatacag gcaaccatgc ctgacttgaa cacagaggag
     1021 tccgcaatct tgtttatatc tgctttacag aaaagggtaa tgaatccgcc aacaagtagc
     1081 atgagactca taatagccgc atcacgacta acaacaacgt tatcaatcag acccagcgtc
     1141 gggctaatgg ccgaagcgta aaggaccacc aggatgacgc caataaggaa aattgcgaca
     1201 gatcgtctgg cataaggctt gagctgtttg ttaacactct ttactggcgc gcttacgagt
     1261 cctttggaaa gacgctcccg gtaaaccgga tcattgtcta atttcatgtc agaaatcaga
     1321 ctgattataa atgcggtaag catgcaacca acgaatgtgg taaacaacca tatcccaagc
     1381 aatactggat aactccaccc aaagccttcc agtacgccag acatatagat aacggcagcc
     1441 gatacaggtg atgctgtaat tgcaatttga gatgatacta ctgcaattga gagcggacat
     1501 gagggcttaa tgttttgttc tttggaaact tcgacaatta cagggatcat tgaaaatgca
     1561 gtatgtcccg taccggcaaa gattgtcagc acataagtga caaccggagc caggtaatta
     1621 atatattttg gatttttacg aagaattcgt tcagcgacct gaacgagata gtccagtcct
     1681 cccgccagtt gcatggccga aatagcagaa attaccgatg caataattag aatcacatcc
     1741 caggggatat taccaggctt catatccaga caaagcccca gaatcaatac cccaatgcca
     1801 ccggcataac caataccaat cccgcccata cggacaccca aaaagattgc ccccagcaga
     1861 accactatct gaagaacagt gataatatcc atgattctct cccgaattag tgagacacct
     1921 ttacccctga gctcctctca acaaaggcct tatggaatgt atttttacaa acatccgggc
     1981 agaggactga tacgttttca ttcgcgccat cccctgccgg atctcccttc ttacagattc
     2041 tgaacaatta ttgttcgaat ttcggagcaa tcagattatc cagattgaat gtcttctccc
     2101 attcttcctg tgtgagtagc ttacgttcga caaccactat ctgatggagg gatttgcctg
     2161 tatggtatgc ttcgtgcgcc atttcagaac acaccttata tcccagatgt ggcttcagga
     2221 gagtcacaat accgcaggaa tgcattacca tctctgcagt tcgatcggaa ttggctgtaa
     2281 tgccatcaat gcatttagtt cggagtgtgt tacaggcatt cgtcatcacc ttgagtgagg
     2341 tgaacagcgc aaaggtgata accggctcca taacgttaag ctcaagctga cctgcagatg
     2401 ccgccagcat cactgtggtg tccagaccct gaaccaggaa gcaagactgg tttgttactt
     2461 ccgggataac agggttaacc ttaccaggca taatggaaga acccggttgc aattgaggca
     2521 ggttaatttc agccaggccg caacggggac ctgaagcaag cagtcgaatg tcattacaaa
     2581 ccttggtaag cttcacagag aggctcttca tggccgaact gagtgcaacg tatgcaccac
     2641 agtcgcttgt ggcagcgatg agatcttcac tgttcctgaa attaatgcct gtgatctggg
     2701 ccagatattt cacggctaaa gcgggataat ccgggtgagc ggtaacacag gtaccaatcg
     2761 cagtagcacc aaggtttaca acccgcaggt gttcctgtgc attacggatg acccgaattt
     2821 catcattaat tgtaaaaccc cagccatgaa actcctggcc gagggtcatt ggcactgcat
     2881 cctgcaggtg agtacgtccc atcttgagca ctctgctgaa ttcgtcagcc tttttgtaga
     2941 atgccccaac aaggtgttca acagccccaa gcaatacatt gcttcgcagc agcaatgcca
     3001 gatgtaatgc tgtcggatat gtatcattgg tggactgacc aaaattagca tggtcgtttg
     3061 gcgaaacgcg tttgtaatcc cctttaacgc caccaagttt ttctgcagca agattacaaa
     3121 tcacttcatt gcagttcata ttggttgacg ttccggctcc cccctgaagc cagtcggtca
     3181 caaactgttc ccggtactta ccatcaatta attggtcgca agcccagatt aaagcatccg
     3241 caacatcagc aggaattgta cctaactcct tattagccat cgcagctgct tttttcacat
     3301 aaccgaaggc aatgatgaag aaaggctcct gactcattgg catttcagtg atatggaaat
     3361 tatcttttcc acgaagtgtc tgaacaccat aatagcaatc atccggaatt tcacgctcac
     3421 caaggaaatc gctttcaata cgagacataa tattctaact ccattatttg aaaacaatgt
     3481 gacaataaca aattgtttaa ccggagctat gctctcggaa tttgaattct tacatcattg
     3541 aaataacatg tgatatcaat ctgtcagaat tcttctgaca gtcagtaact gaatgcagat
     3601 taactgcctg actaattatc aaatagtaac tctgtaacta cttccataaa gaatgaaaat
     3661 tatgattcta tcagaccatc attggtgcaa tcacaaaccc aaatgtcact cctgcaataa
     3721 caccacccaa cccgggtagc ataaatgggt gatttaataa ataattgccc acacgggttg
     3781 tgccggtagt atcaaattcc atcgcggcga gtgatgttgg atacgttgga agcacgaata
     3841 ctcccgtaac ggcaacataa gatgcaagaa ttgcccaggt tggtatgttg agtgctacag
     3901 ccagaggaat aattaacggc gtagtcgctc cctgtgagta aagtaaagcg caggtaaaaa
     3961 aaagaactaa tgctaaaagc atcggataag tttgtaaaat atcaccagcg atatctttga
     4021 tctcagttaa atgcgcatca ataaaggtgg tacctaaggt tacaatacca agaatcactg
     4081 ccagcgaact cattccagca cgaaatgtgg aggtcagaat aatgtcatca gtagacatct
     4141 tacacagtat taccatcagg caggcagcag agagcatgca aataataata atatcgcgcg
     4201 tttccatcgg tttgctgata tcaaaacccg gacgcaattg ggggaatgca gcaagaataa
     4261 caatggcgat cgtcgccagc atgaataatc cgacggaaag cgttgcacca ggtttgatgc
     4321 tgttattatt ctcgtatttt tgcaccagac ctttttgcag acgttcaaga tatacttcgt
     4381 catcttgtaa ttcacacccc tgtcgggagg cgataaaagc agcaaccatc gcggcgacaa
     4441 aagaggtggg aaggcatacc gacatcacct gaataaaact gacgccatta acctccataa
     4501 tagtgagcat agctgccatt gcggcactga ttggtgagcc agaaattgca acctgtgagg
     4561 cgacaactga gccagcgagt gtgcgggaag gccggatacc ggactctttt gctacttcag
     4621 caattaccgg cagtgtggag aaaacaataa acccggtacc ggccataatg gtcattaacc
     4681 aagtgataat cggtgcgatt atgttgatat attttgggtt acgtcgcatg aaattactgg
     4741 ctaaacgtac cagataatcc atcccacctg ccgcctgtaa ggctgaagct gccacaacta
     4801 cggtcataat aatcaggatc acatcgactg gcggtgatcc cataggcaga ccaaatccca
     4861 gagtcaggat cgcaaggcca agaccgccgc ataaaccaat gccgattcct ccctgacgaa
     4921 taccaaaaaa tattgcgcca agtacaacga taatttccag ccaaaccatg atgttctccg
     4981 ttgtaaatat aatctttgta aaataacttt ctggctgtga tttagaatta ttgagttaaa
     5041 tgttgatctt ttccgatacg attttcatac cattttattc ctgcgcgaac gagtgaagcg
     5101 gttgaatcaa tatagcgtaa aggttgatct ttaacggctt gtgacagaat aatgggaatt
     5161 tccgtacaac caagaatcaa gatctctgct ccgcgggaaa ataaattttc agcctgctct
     5221 tccatcaatt tttgcgcatg tgtaacattt cccgctttaa gtgaataaat gcttttcatc
     5281 acattttttt ggccatctgc atccggggtg atatagttaa accctaacga ttcaattcct
     5341 ttctggtata atcccatata cagcgtggca tcagtggcca ggaggccaat tctagtttta
     5401 ccacagacgc gaacttcatc gaccgtagta tctatgatac ttagtatatc aatatggcaa
     5461 gcatctttta gttctttaaa ccaatagtgt gcggtattac agggaataac aatacattct
     5521 gctcctgcat cttccagttt atgcatatag tcacgcataa cgggtaaagg cgagagcccg
     5581 ttatgcatta atgcatcggt acggtcagga atatccggga tagaagaaat aattaaaggt
     5641 atatgttctt gatctcgttg tgctgctgta aaggtgacaa atttattaaa taagtcgaca
     5701 gtggcggccg gccccattcc accgagcaca ccgattaaac ctttcacaat acttacctct
     5761 cagaaaatca atgctcataa acttaaaaaa ccatttctcc ttttttaaca gtctctaaag
     5821 gctatgagaa atgcaatgat gtacaatgca atgcaagact tgcatgatgt agttttgcaa
     5881 tatacatgaa ccatatacta ttgttgcatg aagacctatc ataatgatga aaataaacgc
     5941 ttttgggaga agtgttaaac tggtttagat acagaaaacg aatggattat
//
LOCUS       DARNVM010000226         5949 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.226, whole genome shotgun sequence.
ACCESSION   DARNVM010000226 DARNVM010000000
VERSION     DARNVM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.226"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(166..1125)
                     /locus_tag="SI621_004397"
     CDS             complement(166..1125)
                     /locus_tag="SI621_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEI4067029.1"
                     /translation="MIKKPVIGISGCLAGSAVRFDGGHKRADFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSTQGNIRMCFSYDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FVERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDKLMAILRKPASRKNHTNVLMHIQGYFSNYLSTRQRKELSEVILNYRSG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPDELALRLMVN"
     gene            1318..1842
                     /locus_tag="SI621_004398"
     CDS             1318..1842
                     /locus_tag="SI621_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEI4067030.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENRFERGLEQVSATYGKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTPTIPDKVKADYARTAQ
                     KLGFNVNELLWVKQ"
     gene            complement(1998..2375)
                     /locus_tag="SI621_004399"
     CDS             complement(1998..2375)
                     /locus_tag="SI621_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEI4067031.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKA
                     EGVVEGMLMVLDLRLEMDADCSK"
     gene            complement(2461..2601)
                     /locus_tag="SI621_004400"
     CDS             complement(2461..2601)
                     /locus_tag="SI621_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HEI4067032.1"
                     /translation="MMFEFYMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2598..2960)
                     /gene="rusA"
                     /locus_tag="SI621_004401"
     CDS             complement(2598..2960)
                     /gene="rusA"
                     /locus_tag="SI621_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HEI4067033.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2957..3247)
                     /locus_tag="SI621_004402"
     CDS             complement(2957..3247)
                     /locus_tag="SI621_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HEI4067034.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDDGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3240..3410)
                     /locus_tag="SI621_004403"
     CDS             complement(3240..3410)
                     /locus_tag="SI621_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067035.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3410..3865)
                     /gene="ybcN"
                     /locus_tag="SI621_004404"
     CDS             complement(3410..3865)
                     /gene="ybcN"
                     /locus_tag="SI621_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HEI4067036.1"
                     /translation="MNLSQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATVAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3862..3963)
                     /locus_tag="SI621_004405"
     CDS             complement(3862..3963)
                     /locus_tag="SI621_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067037.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            complement(4080..4877)
                     /locus_tag="SI621_004406"
     CDS             complement(4080..4877)
                     /locus_tag="SI621_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEI4067038.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            complement(4887..5438)
                     /gene="ybcL"
                     /locus_tag="SI621_004407"
     CDS             complement(4887..5438)
                     /gene="ybcL"
                     /locus_tag="SI621_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HEI4067039.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDALTGSGWWHWTVVNIPATITYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
BASE COUNT         1703 a         1396 c         1148 g         1702 t
ORIGIN      
        1 tattttgact gacatgttta ccaactttaa gttgcatctc aatggtttct tcagcgtaaa
       61 cagagttttt atacaaactg acactctggg tatcatagtg tagtttttac gattgtaaat
      121 atcctgcatg caggaactca tccttttgga tgatatcgca tacaattaat ttaccatcag
      181 tcttagagcc agttcgtccg gatagggatc gaagtaattc tgtgtaagca agtaatcatt
      241 aggatactca cccagataat gcttcagcag agtcaacggc gcaagaagag gcaatgtgcc
      301 agaacgatag ttaagtataa cctcactcaa ctctttacgc tggcgtgtac ttaagtagtt
      361 actaaaatac ccctgtatat gcatcagcac attcgtgtga tttttacgtg atgcaggttt
      421 tctgagaatc gccatcagct tatcacgata cacctcaaag tatgattcaa ggtccgccca
      481 ctcgtgtatt gcagccacaa atggtcccat atctttatag cctgcctgac tatgcgccaa
      541 caactgaagc ttataacgac tatgaaaagc taataactct cttcttgata atttctcctt
      601 gtaaaggtga ttgagctcat gcaaagcaaa aactctttca acaaaattct cacgaagcac
      661 tggatcatgt aatcgcccat cctcttcaac cggtagccag gaaaactttt ccatcaaagt
      721 gctcgtaaat agtcccactc catctttacg acctcgatta ccattttcat catagacacg
      781 cacgcgctcc atgccacagc tgggagattt agcacaaacc acaaaccccg atacatcctt
      841 taatttgtcc atataagaac gactaaactc tgtcattctc tctgtcacat cctcattctg
      901 gtcgtagctg aaacacatcc gtatatttcc ttgcgtcgag cgcacaagac gtagagcagg
      961 acgcggaact ggcagcccta tagccatttc cggacatact ggtctgaatg ttacccattc
     1021 cactaatttg tccattaaaa agtcagctct tttgtgacca ccatcaaaac gaacagcaga
     1081 accggccaaa caaccgctga ttccaatcac aggttttttt atcatattct cccccttgac
     1141 taattcatta acacataaac tgtgtagtgc acggaataaa ttgcctttct ggcgtcatca
     1201 ctgacaattt ttctgttatg gactattcct aatatagtat gaaagttctt taagtgatcg
     1261 gtcgtaatca tctatctttc atacttactc tcaactatca aaagtacagg atttattatg
     1321 aagttatggc ctgtgttgac tggcattgca ctctctttca ctcttatagc atgtaaggcc
     1381 ccgacaccac ctaaaggtgt gcagccgatt acaaattttg acgccaaccg ctacctcgga
     1441 aaatggtatg aaatagctcg cctcgagaac cggttcgaac gtggtctgga acaggtcagc
     1501 gctacttatg gaaaacggaa cgacggaggg attcgtgtac ttaaccgtgg atacgatcca
     1561 acgaaaaata aatggagcga gagcgaaggt aaagcatact ttactggaga tactaaaact
     1621 gcagcgttga aggtttcgtt ttttggcccc ttctatggtg gctataatgt aatcaaactg
     1681 gatgatgagt ataagtatgc tcttgtcagt ggtccgaaca gagaatacct atggattctg
     1741 gcaaggaccc caactattcc agataaagta aaagcagact atgcgcgaac cgctcaaaag
     1801 ttgggattca atgtcaatga attattatgg gttaaacaat aaaatcccta cccgaaataa
     1861 tacttattag aaaaaaacca gcctttgggg aggctggcta aatcaggaaa caagctgtta
     1921 tatgataata actacgttgc gattccaaca tttaaaatgt tagactaatg aaaatcagac
     1981 agcaactttt cctttaatta tttcgaacaa tcagcatcca tctccaatcg gagatccaac
     2041 accatcagca taccctccac tacgccctca gctttctgga gcatcctgcc aacccaacaa
     2101 tcagatcgcc catgcttacg tgcaagcgcc ataaaagtca tgccgccgac ataatagtcc
     2161 accaataaat catgcaaatc gctgttgttc tttttcagac gggccatgca cccgcaaatg
     2221 atcatcgcgt catcgtcaca acattgtggg cgagatttta cttttgaagg aattaatccc
     2281 ttaaaaccgg cggcaatgga cgaccaggtc acatcttcat gattattagc cgcccacgct
     2341 ccccaacgct caagaaccat ctgaatatca cgcatcaact tactccacaa aaatcagacc
     2401 agaacgccaa ttacaagcaa aaatcaacaa aacagtatta gttgattgtt atctctgact
     2461 tcatactcct gctcctgtca gggttttggc gtaattcttc agtattcggt aatcggtcaa
     2521 aacagaaccg gggaaacgat ataatcgcag acgcccccag cggtggcgaa gaagttctgc
     2581 catataaaac tcaaacatca ttcattcccc atttcggtga tggtcagttc cagcctccca
     2641 cctttggtaa caggcatctt cacaacgcgg taatcaacga cctgagcatc atccagccag
     2701 aaacctgctt tggtgagtgc gtcaaaagcg gctttttgca gattatccag gtcacggcga
     2761 cggcgatccg gcatgtggca ctcaatacgg attttcactg gcatagccag gccgatatcc
     2821 agcattgcgt ttttaatgat tcgggtgacg ttatcgcggt atgcctgccc ttctgcgctg
     2881 atgtgtgtgc gcccgcgatt atggcggtaa tagcggttat tgctcggcgg ccagggtagt
     2941 gtgatgcagt aagtattcac gccttaatta ccccctcttt cagccagata acctgtgttc
     3001 tcgccatacc ttccagcgca cattcttttg catatccatc gtcaacaaaa tgcgtgcgac
     3061 ggtcgatttc gtcgtggcag gcagaacatg caatggtggc aatcaggtct ggcggtttga
     3121 taccggtacc gcacaatcca gccagccgga tatgtgccag tactgacgtt tcaggattgc
     3181 cattacatac gccggggatt cttacctggc attcccgacc acgcgctgct tttctcaaat
     3241 cagccatgat tcctccttgc tgccagtcgc aaccattttt tatcaaccag gctggcggta
     3301 tatccgagca gtgttggtat ttcggatggt ttcagctcag gcttacgctt acgacgattt
     3361 ggtactctgt agatgtgtcc gttcatgaca cgaataagcg gtgtagccat tacgcctcct
     3421 gcttgtcgcg cagcagctgg aactcgcagc tctgcggaat agtcaggtgg cagccaatat
     3481 tcaccgccca ggcttcaacc ttacacagga agacatacat ctctccggta tcgagatcgg
     3541 aggtatggcg taacgactgg atagtagtga tttcgccggt tacgacatca accaggtcct
     3601 tggtttcata accgaggtat gtgtgtttga gagcatcttt tacccatgct acggtagcga
     3661 acgatttccc cctgctgatg aggtattcac tgatttcgct gtaccacatg tggctgagtg
     3721 cattctggga aagactgcgt ctctcgcgcc acggtttaag caccatgcga aagcatttgc
     3781 cgtcctccag ataaggctgg atctgctgac cgatagcggt gaagttgccg cgatgtaatt
     3841 tgatgccgtc ttgtgagagg ttcacgcttc acctccgcag aggtcaaacg ctagatgcaa
     3901 agaattgcag gtgcatttct gcatctgtga agggagaaga gagtttggat tgtatgtgcg
     3961 cataaacgtc cccgtttagc gcagaagtca ccggagttgt tcaggctccg gtgacataat
     4021 tatgccgtgt tgatttccca aaatcaaaat cgatagaatt gctccttctt aaaacacttt
     4081 tactctctgg aagcttttct tatctctctt ggtgttatat taaaacgatt atgaaatctt
     4141 tcagtaaaac gagaaggaca cttataacca ttttctctgg caatctcgct tataggtttt
     4201 accgtcgttt gtatagcaga caacgcatta tttaacctca catcgtccag tatactttgg
     4261 aaacttaccc cctcgcttgc tagacggcga tgtaatgtag aaacagaaat gtagagatat
     4321 cgagcaacct tgtttgctgt ccattttgtg ccgggttcgg atagcagcag gttataacaa
     4381 cgacttatca atgattgttt actatatgat aaaagtaaat gattaacatg attcactcct
     4441 aacgaaagta gaacgcccat tgctaagtgc tcctgaattt tagttgagaa gcctcgggaa
     4501 acagatgttt ttagttgctc ccaacaatat attaactcag gattctgagg taaaaagaaa
     4561 cttgttttgt tacgtatttg atcagttacc gtataaagtt tttggaaact ctcaattaaa
     4621 tcaatgggta agtaaagcat ttctgcaaga taaagccctg cttcaggata attctcaata
     4681 taaaattcat aaccacaagg aaataatatt atttgattat tatcaacagt taaagtatgc
     4741 gtctcccaat tgataacttt ctttccctga cggatacgac acaaagctgg cataagaggc
     4801 ttaaccctat gaatctcatg atgtttatgc atccgtattt cttcgatctt taagttagtc
     4861 ttacctcttg ccagcatact ctcaccctac tttatctcat aaactggtgt tatctcagcg
     4921 gttgcgattt tattagcatt aagcatataa ccaactaacg ctccgctgga gttagaatct
     4981 acaggaatct tttcagtttt tagagcccat actttaaact ggtaatgatg tggtttatct
     5041 cctttaggag gacatgcgcc accaaaccca gcatagccaa aatcatttcg gccttgaaca
     5101 gcaccagtcg gcagttttgt tccatcacgt ctccctgcat caacgggcaa atatgttatt
     5161 gttgctggaa tattaacaac agtccaatgc caccaaccac tgcctgtaag tgcatctgga
     5221 tcatatacag ttacggcaaa gcttttggta ccttcaggaa caccagacca ggttaatgag
     5281 ggcgatgtat taccaccttc acacccaaat ccagaaaaga catgagacgt tgtaagttgc
     5341 tctcctgttt ttatttcatt actagtgacc tgaaatgctg cagcctgcgc agaaaatgtt
     5401 atgaatgcca atacagttga aacgataagt gttttcataa aaacctcttt gttatgacct
     5461 atcgttattt tatttgatat tcctttatct cattatgcat aaaggcgcaa tttttatgca
     5521 aaagcaatca caattgtacc cccaacccaa ttatttgcca caatatacac aaagcacatt
     5581 gatactatct aaaaactctg ctttattatt agtaatacct acgaaagtcg gtgttatttt
     5641 ttaacctatc attcaaaata cgtgacatac accattttgc tcataataat ttgtcacgta
     5701 ttttcagtat ttgaatctgc gaccaagagt tctcacctaa caaatgatta agattgtata
     5761 gctcatttac taccccaata cagccgtaca aaactcgctt gtgggagcaa acaaagtaat
     5821 tacccattaa gtttcgtcaa agataattaa ttctgtcttg cactttatca ccatagcata
     5881 acttaaaatc cgagatcatt atttagaaat aaatctcacc atcaaccata tatttgagag
     5941 cacttatcg
//
LOCUS       DARNVM010000227         5939 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.227, whole genome shotgun sequence.
ACCESSION   DARNVM010000227 DARNVM010000000
VERSION     DARNVM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.227"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            245..826
                     /gene="mdaB"
                     /locus_tag="SI621_004408"
     CDS             245..826
                     /gene="mdaB"
                     /locus_tag="SI621_004408"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HEI4067040.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            857..1171
                     /locus_tag="SI621_004409"
     CDS             857..1171
                     /locus_tag="SI621_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEI4067041.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1216..2103)
                     /locus_tag="SI621_004410"
     CDS             complement(1216..2103)
                     /locus_tag="SI621_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEI4067042.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRTVASYINEHREVVFEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPTTALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2100..3047)
                     /locus_tag="SI621_004411"
     CDS             complement(2100..3047)
                     /locus_tag="SI621_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEI4067043.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3058..4095)
                     /locus_tag="SI621_004412"
     CDS             complement(3058..4095)
                     /locus_tag="SI621_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEI4067044.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4104..5087)
                     /locus_tag="SI621_004413"
     CDS             complement(4104..5087)
                     /locus_tag="SI621_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEI4067045.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5084..5893)
                     /locus_tag="SI621_004414"
     CDS             complement(5084..5893)
                     /locus_tag="SI621_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEI4067046.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINSLTGKPFFMPFRGVEKV"
BASE COUNT         1671 a         1629 c         1375 g         1264 t
ORIGIN      
        1 acaccacaat tatcaactcg cagaaagtcc acaccaacgt tgtaaattaa ccctgtgcta
       61 gttggaatga tgagaaattg ctatcagtat agtcattttc agaaaatatt tatcctgcat
      121 cggtgagtca gagcaagatc agacttttgc taaattcgca aaagactttg cacattttgc
      181 taatttcacc gtaccgctct gtgacgtact atagtcggca aacgtctcac cttgaggtta
      241 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
      301 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
      361 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
      421 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
      481 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggtcg
      541 tacccgcaaa gatccgtcga aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
      601 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcagttctt
      661 ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
      721 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
      781 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
      841 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
      901 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
      961 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
     1021 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca
     1081 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
     1141 gaatatccgt attctgcaac cggggattta acattcctga aaagaggccg gtgttgctac
     1201 cggcctcagg tcagattagc cattctttct tcccgttttc aaaccccgat ggaaatcgtt
     1261 aattcgctta atggttttaa ccgcacgctg cggcactgcg gaagccaccg accaaacaat
     1321 catttccagg cacaacaata ccgtaccatg tagtggcgtt ttcccctttt catccccgcg
     1381 cggaacatga atcacgatac tggcgtcttt gctaaaacgt gaatccagcg cgtttgtcag
     1441 caaaataaca gggatcccca gccgcctcgc ctcacgaagc gttgtcagcc cttcccggtg
     1501 cgcagatttt tgcgccatca tgatcagaac atcgccacgt tgaagcgcga tcagttgctc
     1561 ggcaaggcca atacccgtac ggtttagtgc cgtggtaggc aaacctatgc ggttgaatag
     1621 tcgtgcggta tagtccgcca ggatacccga agcgccaatg ccaaaaatag cgacttgccg
     1681 tgcctgcacc aacaacgcta cagcttgcgc aagcgcgtgg cggttttcag gctctgacag
     1741 gacattacaa gtatacaaat gcccttcaag aacaaagtcg attgcggtgt taacatcgct
     1801 cgtcaagtta ctcaccgtcg tagacatctt ttcgctggaa ctaagggctg gaccaagcca
     1861 ctgttccaga gtgcgtttca gatcacgcag cccggcaaac cccagagcct gtatagccct
     1921 gataactgtg gcatcggaag tgttcaacgt ggccgcaatt tccatcgccg tttgctcgaa
     1981 taccacttca cgatgctcat taatgtagct tgcgacagtt cttaacccag gcgttaactg
     2041 actcgcgcga gtgcgaaaac gctcaccata aatatcaacc cgcgttttgt ctttctggat
     2101 catttagcct ccggtagagg tcgtcccgca atttgtgatt gtatctgcgc agccatcagc
     2161 cccagagatg caaaggagtt agaaatcgcc tcttcacgag aaatcagcac atagcgcccg
     2221 gaacgacagg ccgtcagaaa ctgacaccat cccggcatga ctttttccat tgctgccagc
     2281 tcatcctgag gtttaccacc ggtgtcaccg cgccaggttg caaaaacaaa atcggcatcc
     2341 agttcaggta aacgctctgc gctcacatcc attcgcccac cttcaggaat gctctcaatc
     2401 agcggcggaa agcgaaaacc agcatcgcgt aatacccgcc ccagcgaatg atagctatgc
     2461 atcacattaa tttttccctg atttgcctga atcaccgata ccgttatttt ttggctgtcc
     2521 agcgtcgcct tcagcgcatt gatttgtgcc tgatagcggc gttcaagtat cgccagctgc
     2581 gactgcgttc ctgtaagttc tgccagcttg cggtagattt ctggcgcgcc tcctttgaga
     2641 tgatcaatgc tcaccgtggg cgcaattttt tccagttgtt caatgggcgt attacgggtc
     2701 ggttctgtga tgatcaggtc aggctttgcc gccacaatcg cttcaatatc aatatcagcg
     2761 gtgccaataa aagcgatcga tgaattatca aagtcgacac ctgtcaataa cgcgcctgat
     2821 cgaatgaaat ggctaccgtc aggccgggtt cgcccgtggc tggcgacagg aggtacaccg
     2881 agctcaatca gaggaatagt gatatcaaga tcgtgtaaag agacaattct ctttggatga
     2941 gcaggaacag tgactttgcg ccctaaatca tcagtaaaga cctgaacagg ttctgtcgcg
     3001 cgggcaaaga atgacaacag tatcagcaga gaaaaaaata aacgcatata actcccatta
     3061 aaaactgttc ctgcgctgcc agagcagtag caaaaagaat ggtccaccaa tcaatgaaat
     3121 aatgattccg gcaggcagtt gcaaaggcaa aaaagccaaa cgaccaatgc tatccgccag
     3181 gatgactaac aacgcccccg atacggcgct ccccagcagt aatgtcgtct gcccgccacg
     3241 tagaataaaa cgtgatatat ggggtgctat caatccgaca aaaccgatgt taccaacaca
     3301 cgaaacgcag acggctgtca ggattattgg tgcggcgaca cgtaacaacg ccagacgaga
     3361 actattcacc cctaacccgg ttgcaacctg atgaccaagc agagcgatat ccgccgtcct
     3421 ggcggtgaac agcaataaaa gagcggctgg caacatccaa caggctgaaa taccgactaa
     3481 catccagtta gctgcatgca ggcttcccga taaccacatc aatgcagttt gtacatctcg
     3541 aacatctgca gtggtcataa atacaccgat accggcggcg aaaaaccagg aaacaccaat
     3601 accaatcaga acaaatcgcg ggcgggaaat atcgcgtgca caaaaaatca cgattaaagc
     3661 caccaacaag ccaccagcaa gaccagccac tggacgccag aacattccca gcattggaaa
     3721 ctggaaaatc agccacagca ccgccacgct acatccttct ttgacgccga ttaaaccagg
     3781 atcggcaagg ccgttacgcg caatggattg catcgcggcc cccgccatac ctaacatggc
     3841 tccgcatagc aatgccatga tgactctcgg caagcgaata tcctgcacaa tataacgtgc
     3901 gtctgtgctt acgttttctg gagcaaacaa cgctcgtcct acttccgatg ccgggacaga
     3961 aaaagaacca tgcatcagac cgaatatcag tattccaacc gcaaacagaa acagcccacc
     4021 aagcaacacc agatttttag ggcgtaccag tgtcgaaaac ttaccaacac gtagtgggcg
     4081 tagcccgacg ttcattatcc gactcatttg aacatcctcg tcgccataat gacaaaaacg
     4141 ggcgcaccaa ctaacgccgt catgacaccg gtcgccagct catggggcgt gaaaagcgtc
     4201 cgggcaataa tatctgccag taatagcagt aaggcaccga cacaagctga aagagggagc
     4261 agaacgcgca gatccgcaga taccagacgc ctgacaatct gcggtactag cagaccgaca
     4321 aaaccaatgg ggccagcaat cgatacggct gcgccacaga gcaacgcgat cgcaagcaga
     4381 gtgaatgttc gcgtgcggag taccgagacg cccagcccct gcgccatgcg atcacccagt
     4441 gccaacatgt taagagaggg cgcaaggtaa atggctaaca caaccccccc cagagaaaac
     4501 caggcggacg tacccagcgt cgcccaatcc tgccccgcta aatcacctgc tagccatgtc
     4561 cgcatcgcca gcagtgtttg ttcgtcaaga atcaggattg cagccgttat tgaggacacg
     4621 aacgccgata gcgccacgcc gcataaagta actttcattg gcgttaaccc tgaacgccct
     4681 gcggaggaaa gcagcaatat caacaggaac aataacgcgc cgcccgtaga tgccagcagt
     4741 gggcgtccaa ccggaaaggc cagcccaagc gcactggcag caacgacagc aagagcggct
     4801 ccggcattta agcctaaaat atgtggctcc cccaatgggt tacgaatgac agcctgcaac
     4861 aaagcaccag caactccaag agaagcaccg gtaagcaatg ctgccgctaa acgcggcaaa
     4921 cggagtctta caattacgtt gtgatcgaag ttttgcggtt caaaatgaag aaatgcctgc
     4981 aacaccgttt gtggtgcaat aaatcgggca ccaatccctg gatataccac gcatcccacc
     5041 agcagtaagg cgaggaaaat tacagtaacc agccctgtgc gcatcatact ttttctacac
     5101 cacgaaacgg catgaagaac ggctttcccg tcagagaatt tattgatgcg tgaacatcaa
     5161 cgtcaaagac agcttttacc agctcaggcg tgcaatgctc cccctcattc aacacacgca
     5221 ccactttccc ctggcgtaaa aatatcaacg tatcaccata attgacggca aaattcagat
     5281 catgcaacac cacgacgact gtacgcccgt gatgacgcgt taaatcatgt aataactcaa
     5341 gaatttcaac ctgataacgt aaatcaagcc aggttgtggg ttcatccagc aggatataag
     5401 gcgtcttttg cgccaacacc atcgcaatcc agcaacgctg gcgctgccca ccagaaagtt
     5461 tttcaacagg taaatgtgca aactcctgcg taccggttaa tttcagggcc tcttctaccg
     5521 cctgctcgtc ggcgtcgctc cattgacgaa tgaaattctg ccagggaaaa cgcccacgcg
     5581 acaccagttc ataaacggtt aagccctccg gcaataatgg cgactgcggc aagatcccaa
     5641 gtctacggga taacgctttc gttggttgct cgtgaatcgc tttaccatcc agcaatatcg
     5701 agccgcccat aggttgcaag atacgtgcaa tggtgctgag caacgtcgac tttccacagc
     5761 cgtttgcccc aaccagaacg gtcatttttt ctgtgggaac ggagaaagaa acaccatcaa
     5821 caatgatttt cttgtgatac ccggcagaaa cattgtcgag gatcagcccc tgcccttttg
     5881 cgttatgtgc cacgtgtacg ccaacttaat caaagacaaa caaaaataaa actcattct
//
LOCUS       DARNVM010000228         5938 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.228, whole genome shotgun sequence.
ACCESSION   DARNVM010000228 DARNVM010000000
VERSION     DARNVM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.228"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..505
                     /locus_tag="SI621_004415"
     CDS             <1..505
                     /locus_tag="SI621_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEI4067047.1"
                     /translation="KCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVN
                     IISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVG
                     AAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPN
                     TKCPEKS"
     gene            521..1060
                     /locus_tag="SI621_004416"
     CDS             521..1060
                     /locus_tag="SI621_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HEI4067048.1"
                     /translation="MKKVFLCAILTSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="SI621_004417"
     CDS             complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="SI621_004417"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEI4067049.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     TRIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="SI621_004418"
     CDS             complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="SI621_004418"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEI4067050.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4429..5799
                     /gene="xseA"
                     /locus_tag="SI621_004419"
     CDS             4429..5799
                     /gene="xseA"
                     /locus_tag="SI621_004419"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEI4067051.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5796..>5938)
                     /locus_tag="SI621_004420"
     CDS             complement(5796..>5938)
                     /locus_tag="SI621_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEI4067052.1"
                     /translation="EFIEMKALCPDCHQPLQVLKACGAVDYFCQHGHGLISKKRVEFV
                     LA"
BASE COUNT         1506 a         1579 c         1536 g         1317 t
ORIGIN      
        1 aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat gccagtcaaa
       61 tgctgatgat catgccgccg atgtttatca aaccgatcaa ctgaatacca aacaagaaac
      121 taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa
      181 taaacggaac gcacaagcct tcggcgcgct tattggtgca gtcgctggcg gtgtaatcgg
      241 ccacaacgtg gggtctggca gcaattccgg aacgacggca ggtgcagttg gcggcggagc
      301 tgttggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag gtgtttctct
      361 aacgtataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag agtgccagtt
      421 tacgacaggt ttagccgttg ttattaccac gacgtataac gaaacgcgta ttcagccaaa
      481 tacgaaatgt cctgaaaaga gctaatgatc aggaggagtc atgaagaaag tttttctttg
      541 cgccatctta acctccttaa gctatccggc tatcgcctca tcattgcagg atcaactctc
      601 tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc atgacgaatg
      661 ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa acgcccaggc
      721 cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc aggataaact
      781 ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg agctacgcag
      841 cttagagatt cagaaacaaa aactggcgct ggcgaaagaa gaagcccgcg ttaagcgaga
      901 aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg tggtgcaatc
      961 tgaagctgac gcaaacagga atatgactga gggcggtcgc gatctgatga aaagcgtggg
     1021 caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgtaag taacttcaag
     1081 cctataattc ttgaagataa aaaaccctct gtagtaacag agggttttgt tcattcatag
     1141 tgcagggatc aaaatcattc ccactcaatg gtagctggtg gcttgccgct gatgtcatac
     1201 accacgcggg aaataccgtt cacttcattg ataatgcggt tggaaacgcg accgaggaaa
     1261 tcgtacggca gatgcgccca gtgtgcggtc ataaagtcga tggtttcgac agcacgcaga
     1321 gaaacaaccc agtcatactt acgaccatcg cccattacgc caacggaacg taccggcagg
     1381 aagacggtga atgcctggct gactttgtcg tacaggtccg ctttacgcag ttcttcaatg
     1441 aagatggcgt cagcacggcg cagcaggtca caatactctt tcttcacttc acccagaaca
     1501 cgaacgccaa ggcctggtcc cgggaacggg tgacggtaca gcatgtcgta cggcaggccc
     1561 agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg ttcaaccagg
     1621 cccatcttca tctctttcgg caggccgccc acgttgtggt gagatttgat gacgtgtgct
     1681 ttaccggttg cagacgccgc agattcgata acgtcagggt agatagtacc ctgcgccagc
     1741 cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa aacgcgcccg
     1801 atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacag gaagcgatct
     1861 tctgccggaa cgtgaacaat attgagacca aagtgatcgc caaacatatc cagaacctgc
     1921 tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag gtttttaccg
     1981 atagcgcggt gcagcagcat tgcggttacg gaggaatcca caccaccaga gaggccgagg
     2041 atgactttat cgtcgcctac ctgctcgcgg atgcgagtta cagcatcgtc gataattttc
     2101 gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc cagcatgcgc
     2161 ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa gcgtttttct
     2221 tcgttagcca taatggcaaa cgggcagctt tcggtgctgg ctacggtgac gaagtcggac
     2281 gggatagcgg taactttatc gccgtggctc atccagacat cgagcagagg tttaccgtct
     2341 gcggtcagag catcttcaat accgcgaacc agtgcgctgt cgtttacgac ttcaacctgc
     2401 gcgtagccaa attcacgttc gttagaggct tcaacgtgac cgcccaactg cattgccatg
     2461 gtctgcatgc catagcaaac gccgaatacc ggtacgcctg cttcaaagac atactgcggc
     2521 gcacgcgggc tgttttcttc ggtggtgctt tccgggccgc cggaaagaat aatgccgctt
     2581 ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc gcagtaaacg
     2641 cccagctcac gcacgcggcg cgcaaccagt tgagtatact gagaaccgaa gtccagaatg
     2701 aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc aagtgaaaca
     2761 gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc agacggtagt
     2821 tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaatg cccgcaccgc
     2881 tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag ccggtcagcc
     2941 ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg cgacctttat
     3001 aggctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgttg tcgctctgga
     3061 agtaacggtc agaagaacct ttggacatcg cgcccaggga acccatccca cggtaagatt
     3121 tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta cccgccagca
     3181 tggaacccac cattaccgcg cttgcgccag cggcgatagc tttggcgata tcgcctgaga
     3241 aacggatacc gccgtcagcg ataaccggaa taccggtgcc ttccagggct tctactgcat
     3301 cagcaacggc ggtgatctgt ggaacaccga cgccagtcac gatacgagtc gtacagatag
     3361 agcctgggcc gataccgact ttaaccgcgc tgcaaccagc ttctgccaga gcgcgtgcac
     3421 ctgcagctgt tgccacgttg ccgccgatga tttgcagatc aggatattta gcacgggttt
     3481 cacggatacg ttgcagaacg ccttctgagt gaccgtgaga ggagtcgatc agcagaacgt
     3541 caacgcctgc ggcaaccagc gcgtcaacac gctcttcgtt acccgcaccc gcgccaaccg
     3601 ctgcaccaac acgcagacgg ccttgctcgt ctttacaggc gttcggttta cgttccgctt
     3661 tctggaagtc tttcacggtg atcatgccga tcaggtggaa ttcgtcatca accaccagcg
     3721 ccttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct tcgccttcac
     3781 gcacggttac cagacgctct ttcggcgtca tgtaaacgct aaccggttga ttcagatcgg
     3841 tcacaaaacg cacgtcacga ccggtgatga tgccgaccag ttcgttttct tcggtaacca
     3901 ccgggtagcc cgcaaagccg ttacgctcgg tcagttcttt cacttcacgc agggtggttg
     3961 tcggcagcac ggtctgcgga tcagttacca cgccagattc gtgttttttc acacggcgaa
     4021 cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata ccgccttcct
     4081 gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg gaaagcatag
     4141 ggatattcag acgaatagtt ttcgtcagct gggtgctgag gtcagcagta ttcggcagaa
     4201 cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct ttagcgatac
     4261 gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc attatacaga
     4321 gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc ggagggcggg
     4381 ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat gttaccttct
     4441 caatcccctg caatttttac cgttagtcgc ctgaatcaaa cggttcgtct gctgcttgag
     4501 catgagatgg gacaggtttg gatcagcggc gaaatctcta atttcacaca accggcttcc
     4561 ggtcactggt actttacact caaagacgac accgcccagg tacgctgcgc gatgttccgc
     4621 aacagcaacc gccgggtgac cttccgccca cagcatgggc aacaggtttt agttcgcgcc
     4681 aatattacgc tctacgagcc gcgcggtgac taccagatta tcgtcgaaag tatgcagcct
     4741 gccggtgaag ggttgctgca acaaaaatac gaacagctta aagcgaaatt gcaggctgaa
     4801 ggtctgttcg atcagcaata caaaaaatca ctcccctctc ctgcgcattg cgttggtgtg
     4861 atcacctcaa aaaccggtgc tgcgctacat gatattttgc atgtattaaa acgtcgcgat
     4921 ccttctctgc cggtgatcat ctaccccacc gccgttcagg gcgatgacgc gccagggcaa
     4981 attgttcgcg ccattgaact ggcgaatcag cgcaacgagt gcgacgtgtt gatcgttggg
     5041 cgcggcggcg gttcgctgga agatttatgg agttttaacg acgaacgtgt agcgcgggcg
     5101 atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc atgagacgga tgtgacgatt
     5161 gccgactttg tcgctgatct gcgtgcgcca acgccgtctg ccgccgctga agtagtgagc
     5221 cgcaatcaac aggagttact gcgtcaggtg cagtctgcgc agcagcgcct cgaaatggca
     5281 atggattatt atctcgccaa ccgcacgcgt cgttttacgc agatccatca tcgcttacag
     5341 cagcagcatc cacagctccg gctggcacgc cagcaaacca tgcttgagcg cctgcaaaag
     5401 cggatgagct ttgcgctgga aaatcagctt aagcgtgccg ggcaacagca gcagcggtta
     5461 acacagcggc taaaccagca aaatccgcag ccgcgtattc accgcgcaca gacgcgaatt
     5521 caacaactgg aatatcgcct ggcagaaacg ttgcgcgcgc aactgagcgc aacgcgagaa
     5581 cggtttggta atgcagtaac gcacctggaa gcagttagcc cgctttcgac gctggcgcgt
     5641 ggatacagcg ttactactgc tactgacggc aatgtactga aaaaagtgaa gcaagttaaa
     5701 gcgggtgaaa tgctaaccac acgtctggaa gacggctgga tagaaagtga agtaaaaaac
     5761 atccagccgg taaaaaaatc gcgtaaaaag gttcattaag ccagcacgaa ttcaacgcgt
     5821 tttttcgata tcaagccatg cccgtgctga cagaagtaat ctactgcacc gcaggctttt
     5881 aacacctgca acggttgatg gcagtctgga caaagagctt tcatttcaat aaattccc
//
LOCUS       DARNVM010000229         5881 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.229, whole genome shotgun sequence.
ACCESSION   DARNVM010000229 DARNVM010000000
VERSION     DARNVM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.229"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            237..947
                     /locus_tag="SI621_004421"
     CDS             237..947
                     /locus_tag="SI621_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEI4067053.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            989..3640
                     /locus_tag="SI621_004422"
     CDS             989..3640
                     /locus_tag="SI621_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEI4067054.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFITSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQSFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGKNKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3654..4184
                     /locus_tag="SI621_004423"
     CDS             3654..4184
                     /locus_tag="SI621_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4067055.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4197..4700
                     /locus_tag="SI621_004424"
     CDS             4197..4700
                     /locus_tag="SI621_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEI4067056.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4759..5673
                     /locus_tag="SI621_004425"
     CDS             4759..5673
                     /locus_tag="SI621_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4067057.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT         1711 a         1260 c         1290 g         1620 t
ORIGIN      
        1 cggtatgaaa aaatcacata atatctaaac gatcaataaa caacagtagt taaacttact
       61 gttatttaaa atatttactt tgtagcaagg ttttcctatt aaaagagaac caacatttat
      121 tacaaacgca ttaagtctgg aaacccggcg agtcgccaat gtattagcaa tatttactga
      181 ttgtctgatg taacggtttt cccgcttatc ctcttaacac atggagaatg catcagatgc
      241 aaactacgcg taccccctac tctatttcct ttatggcgac agtgttactt ttattgctct
      301 ttgcttgcca tagcaccatt gccaacgcag ctgtcgcact gggtgcgacg cgggtaattt
      361 atccggcaaa ccagaaacag gttttattgc ctgtcacgaa taatgatcct gccagtgtct
      421 atctcattca gtcatggata gaaaatgcag gtgaccaaaa agacacccag tttgtcatta
      481 cgccaccgct tttttccatg caaggtaaaa aagagaatac gctgcggata atcaacgcca
      541 ctaatcatca gttaccaggc gaccgtgaaa gcctgttctg ggttaatgtt aaagctattc
      601 ctgctatgga aaaagatcag aagaatgaga atacattaca gctggcaatt attagccgca
      661 taaaaatgtt ttatcgcccc acgaacctgg caatggcacc tgaagaagcg cctgcaatgc
      721 ttcggtttcg tcgcagcggc agcaaactca ctcttattaa tccaacgccc tacttcatta
      781 ccgtaacaaa tatgaaagca ggaaatagca atctcccaaa caccatggtg ccgccaaaag
      841 gtgaagtatc ggtggatatc ccgcatgctg caaccgggga tatcagtttt cagacgatca
      901 atgattacgg tgcattaact ccgcgtatta aggcaaccat gcagtaagcc agtgacctaa
      961 ttaataataa actcaatcgg aattaataat gacagccttt cgtgccgcat ttaaggcata
     1021 tcgtatgcat caggtgttac tactgcctcg ctttgcacgg cttaccattg cccttggctt
     1081 agcaactgcg gtatttcccg ttgacgcaga gtattatttt aatccacggt ttttaagcaa
     1141 tgatcttgct gaatctgtcg atttatccgc ctttactaag ggacgcgagg caccacctgg
     1201 tacatatcgg gtcgacattt atttgaacga cgaatttatt actagtcggg atattacttt
     1261 tattgcagat gataataacg cagatctcat cccatgcctg agtacagatc ttctagtcag
     1321 tcttggcata aaaaaatcag cgttattaga caataaagaa cattcggcag aaaaacatgt
     1381 gccggacaac agcgcatgca caccacttca ggatcgtttg gccgacgcat caactgagtt
     1441 tgatgttggt cagcaacatc tgtcactctc cgttccgcaa atttatgttg gcagaatggc
     1501 tcgcggctat gtttctcctg atctgtggga agaaggaata aatgctgggc ttctaaacta
     1561 tagttttaat ggtaattcta ttaataatcg tagtaaccat aatgcaggaa aatccaacta
     1621 tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactgc gcgataactc
     1681 aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat ggcagcatat
     1741 caatacgtcg gctgaacgtg acattattcc cttacgctca cgcttaacgg taggtgatag
     1801 ttataccgat ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa taaattcaac
     1861 agaagcgatg ttgcccgata gccaacatgg tttcgctccg gtgattcatg gtattgcccg
     1921 cggcaccgca caagtgagtg taaaacagaa tggatacgat gtttatcaga ctactgtccc
     1981 acccggccct tttactattg atgatatcaa ctctgcggcc aatggtggtg atctgcaagt
     2041 aaccataaaa gaggcagacg gcagtattca gacattatat gttccttatt cgtctgttcc
     2101 ggttctccaa cgtgctggat atacgcgtta tgcgcttgcc atgggggaat atcgtagtgg
     2161 aaataacctg caaagctccc ccaagttcat acaaggtagc ttgatgcatg gactggaagg
     2221 aaactggaca ccttatggcg gaatgcaaat tgcagaagat tatcaggcct tcaaccttgg
     2281 tattggtaaa gatttaggac tttttggtgc cttttctttc gatatcacgc aggccaatac
     2341 gacacttgca gatggcaccc gtcacagcgg gcaatcgatt aaatccgtct acagcaaatc
     2401 cttctaccag acgggaacca atatccaggt cgcaggatat cgctattcta cgcaaagttt
     2461 ttataactta tccgacagtg cctacagtcg aatgagtggt tacaccgtca agcctcctac
     2521 cggagacagc aacgagcaga cacaatttat tgattatttt aatctgttct acagtaagcg
     2581 tggtcaggaa caaataagca tctctcagca gcttggaaat tacggtacga cattttttag
     2641 tgccagtcgc caaagttact ggaacacgtc acgcagcgac cagcaaatat catttggatt
     2701 aaatgtgccg tttggtgata ttacgacttc gctgaattac agctattcca ataatatatg
     2761 gcaaaacgat cgggatcatt tacttgcttt tacgcttaat gttcccttca gtcattggat
     2821 gcgtacagac agtcagtcgg catttcgtaa ttcaaacgcc agttacagta tgtcaaacga
     2881 tttgaaaggc ggcatgacca atctatcggg agtttatggc actctgctgc cggataataa
     2941 cctgaattat agtgttcagg tcggtaacac ccacgggggt aatacatcgt ctggcaccag
     3001 tggttacagt tctcttaatt atcgtggagc ttacggtaat acgaatgtcg gttacagtcg
     3061 gagtggtgac agcagccaga tttattacgg aatgagtggt ggcattattg ctcatgctga
     3121 tggcatcacc tttggacagc cgctgggcga cacaatggtt ctggttaagg ctcctggcgc
     3181 tgataatgtc aaaatagaga accagaccgg tattcatacc gactggcgtg gctatgccat
     3241 attaccattt gcgacagaat atagagaaaa tcgtgtcgct cttaacgcga attcccttgc
     3301 agataatgtt gaactggatg aaaccgtggt cactgtcatc ccaactcacg gtgctattgc
     3361 cagagcaaca tttaatgcac aaatcggcgg gaaagtatta atgacgttga agtacggtaa
     3421 taagagcgtt ccattcggtg caattgtcac tcacggaaag aataaaaatg gcagcattgt
     3481 cgcggaaaac ggtcaggttt atctgactgg acttccacag tcagggaaat tacaggtttc
     3541 atggggcaaa gataaaaact caaactgtat tgtcgagtac aagcttcctg aagtttctcc
     3601 tggtacctta ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag attatgaaat
     3661 acaataacat tattttcctc ggtttatgtc tggggttaac cacctattct gctttatccg
     3721 cagatagcgt tattaaaatt agcgggcgcg tcctcgatta tggctgcaca gtctcatcgg
     3781 attcgcttaa ttttaccgta gatctccaaa aaaacagtgc cagacaattt ccaacgaccg
     3841 gtagcacaag tccagccgtc ccttttcaga ttacgttaag tgaatgcagt aaagggacaa
     3901 cgggggttcg ggttgcattt aacgggattg aggatgcaga aaataatact ctgttgaaac
     3961 tggatgaagg aagcaatacg gcctccggtt tgggtataga aatactggac ggaaatatgc
     4021 gtccggtgaa attgaatgac cttcatgccg ggatgcagtg gatcccactg gtaccagaac
     4081 agaacaatat tttgccttac tccgctcgtc tgaagtcaac tcagaagtcc gtcaatccgg
     4141 gactggtgag ggcttcggca acctttaccc ttgaatttca ataatcagag tcagatatga
     4201 aaagacttca caagaggttc ctgttagcta cgttttgcgc gttattcaca gcaactctcc
     4261 aggccgccga tgtcactatc actgttaatg gtcgggtagt cgctaaaccc tgcactattc
     4321 aaaccaaaga agctaacgtt aatctcgggg atctttatac gcgcaatctg caacaacctg
     4381 gttctgcatc aggctggcac aatattactt tgtcattaac cgattgtccg gttgaaacaa
     4441 gtgcagtgac ggcaatcgtg acaggttcaa ctgacaatac gggttattac aaaaatgaag
     4501 gtactgccga aaatattcag atagagctaa gggatgacca ggatgcgacg ttaaaaaatg
     4561 gcgatagcaa aacggttatt gttgatgaga tcactcgtaa tgcacagttt ccacttaagg
     4621 caagagctat cacggtgaat ggaaacgcaa gccagggaac gatcgaggcg ctaatcaatg
     4681 tgatctacac ctggcaataa cgtcgattcg attttcttag cctcttttta aatcaatttt
     4741 ttaaccttag gatatatcat gggtaaaaca ataagtatga agcttctgtt cggtatttat
     4801 cttctattaa tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt
     4861 attggagcag gtacaacctc tgtttatgtt aatctcgacc ctgtaataca accgggccag
     4921 aatctggttg tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac
     4981 gacactgatc atataaacct ggtacaaggc tcagctttcg caggctctct acagtcatat
     5041 aaaggatcgc tgtactggaa taatgtgacc taccccttcc cgttgacgac aaatactaat
     5101 gtattagata ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca
     5161 gtcggtgcgg ccggtggagt tgtcataaaa gccggggaag ttattgcccg tattcatatg
     5221 tataaaattg ccacgttagg aagtggaaat ccgcgtaatt ttacgtggaa tattatttca
     5281 aataacagtg ttgttatgcc aacaggcggc tgcaccgttg atagtcgtaa tgtcaacgtg
     5341 aatctcccgg actttcctgg aagcgcagaa ataccccttg gcgtatattg ctcaagtgaa
     5401 caaaagctct cattttatct ttcgggtgct accactgaca gtgccagaca agtgtttgct
     5461 aacacagcac cagatgcaac aaaagcatcg ggagtgggtg tctctctgat gcgtaatgga
     5521 aaaatactgg ctacgggaga aaacgtctcc ctggggacag taaataaaag caaagtacct
     5581 ctgggtttaa gcgcaaccta tggacaaaca ggaaacaagg ttgcagccgg aaccgtccag
     5641 tcagtcattg gtgtcacttt tatctatgaa taattgtgat tttgacattg gtctatgcgc
     5701 tccattcacg aagattgcgc ggataattat tttgtgaagg ctattatcct acatctgtaa
     5761 gaaaatccgc gcacaattga agccagagtc ttatttttta tattgaaaaa tatgttaatt
     5821 taaacactat gagagatgat gcttaattta tcgtatttaa tcttttgttt aacgataata
     5881 g
//
LOCUS       DARNVM010000230         5816 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.230, whole genome shotgun sequence.
ACCESSION   DARNVM010000230 DARNVM010000000
VERSION     DARNVM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.230"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..798
                     /locus_tag="SI621_004426"
     CDS             <1..798
                     /locus_tag="SI621_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067058.1"
                     /translation="TYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQ
                     VYIHAQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQV
                     ETVGSNQIVKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLM
                     VGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNG
                     THIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            874..5374
                     /locus_tag="SI621_004427"
                     /pseudo
     CDS             874..5374
                     /locus_tag="SI621_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5374..5751
                     /locus_tag="SI621_004428"
     CDS             5374..5751
                     /locus_tag="SI621_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067059.1"
                     /translation="MNQYIANYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQI
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSIDDELATVVATGLIEAIVTATDSNSLLWK
                     DVSKFLGDKSKSHALVWKDFGQE"
BASE COUNT         1431 a         1411 c         1916 g         1058 t
ORIGIN      
        1 acctaccacc aggaaaaccg cacccccggc agcctgccgg gaacaaagac gcagatgacc
       61 attcgttcaa aaacctataa gggcagcggg tttaatgaac tgaagtttga cgatgcgaca
      121 gggaaagaac aggtctacat ccacgcgcag aagaacatga acaccgaggt gctgaataac
      181 cgcaccactg atgtgataaa caaccatgcc gaaaaaatag gtaacaacca ggcgatcacc
      241 gttaccaata accagatcca gaacattggc gttaatcaga tacagacggt tggtgtcaac
      301 caggtggaaa cggtaggcag caaccagatt gtcaaagtgg gttcaaacca ggttgaaaag
      361 gtggggatca ttcgtgcgct gacggtgggc gtggcttacc agacgacggt aggcggcatc
      421 atgaatacct cggtggcgtt gttgcagtcc tcacaggtag ggctgcataa atcactgatg
      481 gtgggaatgg gctacagcgt caatgtaggg aataacgtca ccttctcggt gggcaagacg
      541 atgaaggaaa acaccggaca aacagcagtt tattctgccg gtgagcatct ggaactctgc
      601 tgcggtaagg caaggttggt gctgacgaag gacggaagca tatttctcaa tggtacgcac
      661 attcatctgg aaggagagtc ggatgtgaac ggtgatgcgc cggtgattaa ctggaactgt
      721 ggtgccacac aacctgtacc ggatgcgcct gtgccgaaag atttaccccc cggaatgccg
      781 gatatgcggc aattttgagt caggtgcaaa aaattaaaac ggaactggtg cagtgaccag
      841 ataaaccata ttaaaagcag aaggaattga ttcatgggtg gaaaaccggc ggcgcggcag
      901 ggtgacatga cccgcaaggg actggatatt gtgcagggtt cagcaggggt gctgataggt
      961 gcgccaaccg gtgtggcctg ctcggtgtgt cccacagggg aagccacgcc gaaatatggc
     1021 aatccggtaa acccgctgct gggagcgaag gtgctgcctg ctgaaaccga cctggcactg
     1081 tccggcccgc tgccgtttgt tcttttccgc gcctacagca gctaccggac caggacgccc
     1141 gcgccggtgg ggggggttgg tcccggctgg aaagccccct tcgatatccg cttacagata
     1201 cgcgatgaag gcctgatact caacgacagc ggcggcagga gcattcactt tgaacccctg
     1261 tttcccggcg agataagcta cagccgcagc gagtcgttct ggctggcgcg gtgcggggtg
     1321 ctgaaacagc acaaaggcca cccgcttgcg cggctctggc gggcgttgcc ggaagcggtc
     1381 cgtctgagtc cacacatcta tatgatggct gccagcacaa ccgggcagtg gctggtgctc
     1441 ggctggccgg agcgggtgcc gggggcggac gaggtgctgc cgccggagcc gcccgcatac
     1501 cgggtgctga cgggcgtggt ggacggcttc gggcggacgc tggccttcca ccgggcggca
     1561 gagggtgacg tggcgggtgc ggtgacgggg gtgacggacg gcgcgggccg ctgttttcac
     1621 ctggcgctga gcacgcaggc gcagcgggcg gaagccttcc gtaaacagcg cgcctcttct
     1681 ttatcttctc cagccggtcc ccgttctgtt tcctcttctc aggttttccc cgatacgctg
     1741 cccgccggta cagaatacgg tgccgataac ggtatccggc tggaggcggt gtggctgaca
     1801 cacgacccgg catacccgga tgaacagccg actgcgccgc tggcgcgcta tacgtacacg
     1861 gccagcggag aactgcgggc ggtgtatgac cgcagcggga cgcaggtgcg cgggtttact
     1921 tatgatgcgg agcacgccgg gcggatggtg gcgcaccatt atgcagggag gccggagagc
     1981 cgctaccggt atgatgatac cgggcgggtg acggagcagg tcaacccgga ggggctggac
     2041 taccgctttg agtacgggca ggaccgtgtg accatcacgg acagcctgaa ccggcgggag
     2101 gtgctgtaca cggaaggcgg gggtggcctg aagcgtgtgg tgaagaagga gcatgccgac
     2161 gggagcatca cccgcagcga gtatgatgag gcgggggagg ctgaaggcgc agacggatgc
     2221 ggcaggacgg cggacggagt accggctgca tatggcgtct ggaaagctga cttcagtgat
     2281 actgccggac ggcaggacgg tacggtatgg ttacaacagc cagttgcaac tgacttcagt
     2341 gacatatccg gatggtttac gcagcagccg gaaatatgac cgtcagggcc gactggcaga
     2401 agagatctcg cgcaacggta acattacgcg ctggttttat gactcccccc gcagtagcct
     2461 gcccggcgcg gtggaagacg gaatcggggt gacgcggaga ataacgcgaa accggtacgg
     2521 tcagcttcgg gccttcacgg actgctcggg gtacatgacg cggtatgagt atgaccggta
     2581 cggtcaacaa atcgccgttc accgggagga aggcatcagc acttacagca gttataaccc
     2641 gcgaggccaa ctggtcagtc agaaggatgc gcaggggcgt gaaacccgtt atgagtacag
     2701 cgccgcaggc gacctgacag caacgatatc accggacggt aaacgcagca ccatcgaata
     2761 tgataaacgc gggcgtccgg tgagtgtgac ggaaggcggt ctgacgcgca gcatggggta
     2821 tgacgctgcc gggcgcatca ccgtgctgac caacgagaac ggcagccagt ccacgttccg
     2881 gtatgacccg gtggacaggc tgacggagca gcgcggcttt gacggcagga cgcagcgtta
     2941 ccggtacagc gccacagggc agcttgtcca cagtgaagac gagggactta tcaccctctg
     3001 gcattacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc cggcggagca
     3061 gtggcagtat gatgagcacg gctggctgac agaaatcagc catatcagcg aaggccaccg
     3121 tgtggcggtc cactacggcc acgacgataa aggccgcctg acgggcgaac ggcagacggt
     3181 ggagaacccg gagacggggg agatgctgtg ggagcatgag acgaagcacg cgtacagcga
     3241 acaggggctg gcgacccgtc aggagccgga cggtctgccg ccggtggagt ggctgacgta
     3301 tggcagcggt tatcttgcgg gaatgaagct gggtggtacg ccactggtcg agtacacgcg
     3361 ggaccggctg caccgtgaga cggcccgcag cttcggcggg gaggcatatg agcttgccac
     3421 cgcctggaat accagcggcc agctccggag caggcacctg aaccttccgc agcttgaccg
     3481 tgactacgac tggaacgaca acggacagct aattcgcatc agcggcccgc aggagagccg
     3541 ggagtaccgt tacagcgaca cgggaaggct gacgggcgtc cacaccacgg cagcgaacct
     3601 ggacattgat atcccgtatg cgacggaccc ggcaggaaac cggctgccgg acccggaact
     3661 gcacccggac agcaccctca cggcatggcc ggataaccgc atcacggaag atgcgcacta
     3721 tgtctaccgc tacgatgaat acggcaggct ggcggagaag acggaccgta tcccggaagg
     3781 ggtgatacgg atgcacgacg agcggacgca ccactaccat tacgacagcc agcaccgcct
     3841 ggtgttctac acgcggatac agcacggcga gccacaggtg gagagccgct acctctatga
     3901 cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg gagcgtgacc tgacggggtg
     3961 gatgtcgctg tcgcgtaaac cggaggtgac ctggtacggg tgggacggcg acaggctgac
     4021 gacggtacag accgacacca cgcgtatcca gacggtatac cagccgggga gcttcacgcc
     4081 gctcctgaga atcgaaacag agaatggcga acaggcgaag gcgcggcacc gtagcctggc
     4141 ggaggtgttg caggaggaca cgggtgtgac gctaccggcg gagctggcgg tgatgctggg
     4201 gaggctggag cgggaactgc ggcagggcag cgtgagtgaa gaaagccagc agtggcttgc
     4261 acagtgtggg ttgacggcgg aacagatggc ggcgcaactg gaggcggaat acatcccgga
     4321 gaggaaactt catctttacc actgtgacca ccgtgggctg ccgcaggcgc ttatcagccc
     4381 ggaaggggaa acggcgtggc gcggggagta tgacgagtgg ggaaacctgc tgggagagga
     4441 aagcacgcag caccttcaac agtcactccg tctgccgggg cagcagtatg atgaggagtc
     4501 ggggctgtac tacaaccgtc accggtacta tgacccgcta caggggaggt atatcaccca
     4561 ggatccgatt gggctgaagg ggggatggaa cctgtatgcg tatccactga acccggtgca
     4621 acggctggat ccgctgggat taagtgcggc gttgggcgca ggagcattaa ctgccatggg
     4681 aactggttct gaagtaggta cagagatcgg ttcggtagga ggccctccgg ggatgttagt
     4741 cggtgcggca gtcggtacgg tggttattgg tggtggtata ataatataca acaatcgcga
     4801 ggctgttggg gacgcgattg agaaaagtat ggagatgcag ggtgaagccg aattattaaa
     4861 tgcacaaagt caaatggaat tctggcgaga tccctatggt gctttttcca gcgtgtttgg
     4921 aaatgatgat tcatcaaaag agaatccaaa tgtcgggaag gatttgacgg aggaagagaa
     4981 gaactgcctg ggtggtggca gtacgggaag cccgggagga tggggaccag aagatgagaa
     5041 tagcgcaaga gatcgtatgc agcatgaacg gtataaagat gaattgcgac agaagatggg
     5101 aaaacctgac gttacagatt caaatttgaa aaatattata gacgatcttt atcggccaaa
     5161 tgcaaaagta ggaagtggaa gcacagcaga tgctgttcgt tatgaattgg ccacaggtga
     5221 aaaagttggt ggtagaggac atgtattgaa agcacaggat tatacaaggg ctttacagga
     5281 ttggttggat aagaatccaa cagcatctcc aggggatcga gcagcagcag aaaacgttct
     5341 tcaggattta cagaatgctt tgggaggcaa gtaatgaacc aatatattgc taattatata
     5401 gaaaaaatgg catcgttttc cactgttttg tctgagacag tctcattagc taatgaatac
     5461 tggcatccag aaactccacc ggtggttatg ttgttctctc agattggaaa aacgattgta
     5521 aaaaattttt atgaaatggg tgatattgaa agagaaaaaa tatttaaaca tgtcgaagat
     5581 ggtatggtct cgattgatga tgaattggcc acagttgtag ctacagggct tatagaggca
     5641 atagtcactg cgaccgatag caattcttta ctttggaaag atgtaagtaa atttttgggt
     5701 gataaatcaa agtctcatgc attagtgtgg aaagattttg gtcaggagtg atattcttta
     5761 agaagtatta agttcgctat tgaggcctta atacttcttg aagttaaatt aaagtt
//
LOCUS       DARNVM010000231         5813 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.231, whole genome shotgun sequence.
ACCESSION   DARNVM010000231 DARNVM010000000
VERSION     DARNVM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.231"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1528
                     /gene="tssI"
                     /locus_tag="SI621_004429"
     CDS             <1..1528
                     /gene="tssI"
                     /locus_tag="SI621_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEI4067060.1"
                     /translation="EIPWNPNTRTEVSTLCISQFLYSAQIRPSSVVTKDYTFKRPGWA
                     GRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFACWQMDGWRNNAEVARGTSRSPEI
                     WPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGRRGSGTTLNNHFAVIPADRTW
                     RPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQ
                     AWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIR
                     SKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIA
                     VTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVA
                     LMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKA
                     RLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY
                     "
     gene            1595..5377
                     /locus_tag="SI621_004430"
                     /pseudo
     CDS             1595..5377
                     /locus_tag="SI621_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1382 a         1581 c         1875 g          975 t
ORIGIN      
        1 tgagatcccc tggaatccga acacccgtac cgaggtgagc accctctgca tcagccagtt
       61 cctctacagc gcacaaatcc gcccatcttc cgtggtgacc aaagactaca cctttaaacg
      121 acccggctgg gcagggcgtt ttgatcagga aggccagcac caggattacc agcgcacaca
      181 gtatgaagtg tatgactacc ccggacgttt caagggtgcc cacgggcaga actttgcctg
      241 ctggcagatg gatggctggc gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc
      301 ggagatatgg ccgggacggc gaattgcgct gacggggcat ccgcaggcga acctgaaccg
      361 ggaatggcag gtggtggcaa gtgaactgca cggcgagcag ccgcaggcgg tgccgggacg
      421 cagggggtca ggtaccacgc tgaataacca ctttgcggta ataccggcag acagaacatg
      481 gcggccacag ccgttgctga aaccgctggt ggacggcccg cagagcgccg tcgtgacggg
      541 accggcagga gaagaaatct tctgtgatga acatggccgc gtgcgggtga aatttaactg
      601 ggaccgctat aacccgtcaa accaggacag ttcatgctgg atccgtgtgg cacaggcgtg
      661 ggcaggcacc ggctttggca acctggcgat accgcgtgtg ggtcaggaag tcattgtgga
      721 cttcctcaac ggcgatccgg accagccgat tattatgggg cgtacctacc accaggaaaa
      781 ccgcaccccc ggcagcctgc cgggaacaaa gacgcagatg accattcgtt cgaaaaccta
      841 taagggcagc gggtttaatg aactgaagtt tgacgatgcg acagggaaag aacaggtcta
      901 catccacgcg cagaagaaca tgaacaccga ggtgctgaat aaccgcacca cggatgtgat
      961 aaacaaccat gctgagacca ttggcaacaa tcagatgatt gcggttacca acaatcagat
     1021 acagacggtg ggcgttaacc agatagagac ggtgggcagt aaccagatca ttaaagtggg
     1081 ctctgttcag gtggaaacga ttggacttgt tcgtgcgctg actgtgggcg tggcgtatca
     1141 gacgacggta ggtggcatta tgaacacttc ggtggccctg atgcagtcct cgcagatcgg
     1201 tttgcataaa tcgttgatgg tcgggctggg ttatgacgtc aaagtcggaa ataacgttac
     1261 cttcaccgtt ggtaaaacga aaaaggatga taccgggcag accgcgattt actccgccgg
     1321 tgagcatctg gaactctgct gtggtaaggc aaggctggtg ctgactaagg acggacaaat
     1381 ttttctcaac ggcacaaaaa ttcatttgca gggtaaggag caggttaatg gtgactcact
     1441 gttgattaac tggaactgtg cagcctcgaa atccccaccg aagacccctg atgaaaagca
     1501 ggatacgccg gatatgagag agtactgatc ccgtacctgt tcggtattca gtaacaaaca
     1561 agcattaaaa ataaacagaa gaaggaattg aatcatgagc ggaaaaccag cggcgcgtca
     1621 gggagatatg actcagtatg gcggtcccat tgtccagggt tcggcaggtg taagaattgg
     1681 cgcgcccacc ggcgtggcgt gctcggtgtg tccgggcagg atgacttcgg gcaacccggt
     1741 aaatccgctg ctgggggcga aggtgctgcc cggcgagacg gaccttgcgc tgcccggccc
     1801 gctgccgttc attctctccc gcacctacag cagctaccgg acgaagacgc ctgcgccggt
     1861 gggaattttt ggccccggct ggaaagcgcc ttctgatatc cgcttacaga tacgtgatga
     1921 cggactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc tgctgccggg
     1981 ggaggcggtg tacagccgca gcgagtcaat gtggctggtt cgcggtggta aggcagcgca
     2041 gccggacggc cacacgctgg cacggctgtg ggcgtcgctg ccgccggata tccggttaag
     2101 cccgcatctt tacctggcga ccaacagcgc acagggaccg tggtggatac tggggtggtc
     2161 agagcgggtg ccgggcactg aggacgtact gccagcgccg ctgccgccgt accgggtgct
     2221 taccgggctg gcggaccgct tcgggcggac gctgacgtac cggcgtgagg ccgccggtga
     2281 cctggccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc gtctggtgct
     2341 gaccacgcag gcgcagcggg cggaagaggc ccgtaaacag cacaccgctt ccttattttc
     2401 ccctgacacc ccccgccctc tttcagcctc agcgttcccc gacacactgc ccggtaccga
     2461 atacggcccc gacaggggca tccgtctttc ggcggtgtgg ctgatgcacg acccggcata
     2521 cccggagagc ctgcccggtg cgccactggt gcggtacacg tatacggaag ccggtgaact
     2581 gctggcggta tatgaccgca gcaatacgca ggtgcgcgct ttcacgtatg acgcgcagca
     2641 cccgggccgg atggtggcgc accgttacgc gggaaggccg gagatgcgct accgctacga
     2701 cgatgcgggg cgggtggtgg agcagctgaa cccggcaggc ctgagttacc gctaccagta
     2761 tgagcaggac cgcatcaccg tcacggacag cctgaaccgg cgtgaggtgc tgcatacaga
     2821 aggcggggcc gggctgaggc gggtggtgaa aaaagaactg gcggacggca gcgccacgca
     2881 cagtgggtat gacgcggcag gaaggcttac ggcgcagacg gacgcggcgg gacggcggac
     2941 agagtacggt ctgaatgtgg tgtcaggcga tatcacggac atcaccacac cggacgggcg
     3001 ggagacgaaa ttttactata acgacgggaa ccaggtgacg gcagtggtgt ccccggacgg
     3061 gctggagagc cgccgggcat atgatgaacc gggcaggctg gtaacggaga catcgcgcag
     3121 cggggagaca gtacgctacc gctacgatga cgcgtacagt gagttaccgg cgacgacaac
     3181 ggatgcgacg ggcagcaccc ggcagatgac ctggagccgc tacgggcagt tgctggcgtt
     3241 caccgactgc tcgggctacc agacccgcta tgaatacgac cgctttggtc agatgacggc
     3301 ggtccaccgc gaggaaggta tcagccgtta ccgccgctat gacaaccgtg gccggttaac
     3361 ctcggtgaaa gacgcacagg gccgtgaaac gcagtatgag tacaacgccg caggcgacct
     3421 gactgccgtt atcaccccgg acggcaaccg gagcgagaca cagtacgatg cgtggggaaa
     3481 ggcggtcagc accacacagg gcgggctgac gcgcagtatg gagtatgacg cggcaggacg
     3541 tgtcatcagc ctgaccaacg agaacggcag tcacagcgac ttcagctacg atgcgctgga
     3601 ccggctggta cagcagcgcg gctttgacgg gcggacgcaa cgttaccact atgacctgac
     3661 cggaaaactc acgcagagtg aagatgaggg gcttgtcacc ctctggcact acgacgaatc
     3721 ggaccgtatc actcaccgca cggtgaacgg cgaaccggca gaacagtggc ggtatgatgg
     3781 ccacggctgg ctgagagaaa tcagccacct gagcgaaggc caccgtgttg ccgtccacta
     3841 tggctatgac gataagggcc gtctgaccgg ggagcgccag acggtggaga acccggagac
     3901 gggggaactg ctgtggcagc atgagacgaa acaggcatac aacgaacagg ggctggcaaa
     3961 ccgcgtcacg ccggacagcc tgccgccggt ggagtggctg acctacggta gcggttatct
     4021 ggcgggcatg aagctgggcg acacgccgct gctggagtac gcgcgcgacc gcctgcacag
     4081 agaaacggtg cgcagcttcg gcagcatggc aggcagtaat gccgcatacg aactgaccag
     4141 cacatacacc cccgcaggcc agttacagag ccagcacctg aacagcctgg tgtatgaccg
     4201 tgactacggg tggaatgaca acggcgacct ggtgcgcatc agcggcccgc gacagacgcg
     4261 ggaatacggc tacagcgcca cgggcaggct ggcgagtgtg cgcaccctcg caccggacct
     4321 ggacatccgc atcccgtatg ccacggaccc ggcgggcaac cggctgccgg acccggagct
     4381 gcacccggac agtacactca cagtgtggct ggataaccgc atcgcggagg atgcgcacta
     4441 tgtctaccgc cacgatgaat acggcaggct gacggagaag acggaccgca tcccggcggg
     4501 tgtgatacgg acggacgacg agcggaccca ccactaccac tacgacagcc agcaccgcct
     4561 ggagttctac acgcggatac agcatggcga gccgcaggtg gagagccgct acctctacga
     4621 cccgctgggc cgccggacgg gtaaacgggt gtggcggcgg gtacgtgacc tgacggggtg
     4681 gatgtcgctg tcgcgtaaac cggaggtaac gtggtacggc tgggacggtg acaggctgac
     4741 gacgatacag acacaacaga gccggatcca gacggtatac gagccgggaa gcttcacacc
     4801 actcatccgc atcgaaacag acaacggcga gcgggagaaa acgcagcgcc acagcctggc
     4861 ggagaagctg cagcaggaag ggagcgagga cgggcacggc gtggtgttcc cggctgaact
     4921 ggtacggctg ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag
     4981 ccgggcgtgg cttgcgcagt gcgggctgac ggtagagcaa ctggccagac aggtggagcc
     5041 ggaatacaca ccggcgcgaa aagttcatct ttaccactgc gaccaccggg gcctgccgct
     5101 ggcgctcatc agcgaagacg gcaatacggc gtggagcggg gagtatgatg agtggggaaa
     5161 cctgctgaat gaggaaaacc cgcatcacct gcaccagccg taccgtctgc cagggcagca
     5221 acacgatgag gagtcggggc tgtattataa ccgtcaccgg tactacgatc cgttgcaggg
     5281 gcggtatatc atccaggacc cgataggggg ggatggggga tggaatctgt accaatatcc
     5341 gctgaatcca gtgcaacacg ttgatccatt aggccttagt actatgataa taggtaatgg
     5401 ccccgttcct gataatcctt ttgggcatgc tgcggcggca aatcgatatg gattaatgag
     5461 ttcgggaact ggggatgaga tgggcgcctc tgtctctgat tattttaaaa agatgcaacc
     5521 ccgcagagat acctggattt ggataataga tacaacagat gaagaagaac aatgtatgat
     5581 gaataaggca atagacctta ataaaaaact aaaaactatt aacctcgggc ctcttcctgt
     5641 tgcaaataat tgttttagcc gaacgaacat gatttttgag gcgtgcgggt ttaacaaccc
     5701 ctctatgaat acaaatgcgc ctataagcct acaagtgctc ggtgagtttt atggatatca
     5761 aagatatatt atgcctaaag gtcctttcac tggattccca tttaaatttc aag
//
LOCUS       DARNVM010000232         5812 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.232, whole genome shotgun sequence.
ACCESSION   DARNVM010000232 DARNVM010000000
VERSION     DARNVM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.232"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            73..222
                     /gene="hokB"
                     /locus_tag="SI621_004431"
     CDS             73..222
                     /gene="hokB"
                     /locus_tag="SI621_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HEI4067061.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(294..467)
                     /locus_tag="SI621_004432"
     CDS             complement(294..467)
                     /locus_tag="SI621_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067062.1"
                     /translation="MKKLALILVIGALVSFFADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(712..1242)
                     /gene="cybB"
                     /locus_tag="SI621_004433"
     CDS             complement(712..1242)
                     /gene="cybB"
                     /locus_tag="SI621_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HEI4067063.1"
                     /translation="MGNKYSRLQISIHWLVFMLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1431..2432
                     /gene="gap"
                     /locus_tag="SI621_004434"
     CDS             1431..2432
                     /gene="gap"
                     /locus_tag="SI621_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEI4067064.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDIVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILEKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2474..3913)
                     /gene="aldA"
                     /locus_tag="SI621_004435"
     CDS             complement(2474..3913)
                     /gene="aldA"
                     /locus_tag="SI621_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEI4067065.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRKRASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGVRVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAIAMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(4110..4910)
                     /locus_tag="SI621_004436"
     CDS             complement(4110..4910)
                     /locus_tag="SI621_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEI4067066.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(5026..5403)
                     /locus_tag="SI621_004437"
     CDS             complement(5026..5403)
                     /locus_tag="SI621_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067067.1"
                     /translation="MDECFINITPTSLSFKNAELSDLSYKNARLELTLICPAIKSEVK
                     LSFDWIHSFRVTDEGDLLGMSGIHGINQTGIYRVVNSAYFAWFTMQSCEIYKNNVIEH
                     YVIATSNDVIDVLSTVSPQIQYD"
     gene            complement(5523..>5812)
                     /locus_tag="SI621_004438"
     CDS             complement(5523..>5812)
                     /locus_tag="SI621_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067068.1"
                     /translation="LVYWESEQRWTSVNGLEVMSMFDEYCHHIYLDDINKKIQAELPE
                     DKTLGFKRQLQFFILGDEKIRIWVPQGELYFALMNVLQMFPLKKMVDDWTR"
BASE COUNT         1565 a         1429 c         1287 g         1531 t
ORIGIN      
        1 accgaggcca acgaatgaac ctagcaattc agatcttagc ctcttacccg ccatccggca
       61 aggagaaagg ctatgaagcg caaccctctg gtggtgtgtc tgctcattat ctgcattacg
      121 attctgacat tcacactcct gacccgacaa acgctctacg aactgcggtt ccgggacggt
      181 gataaggagg ttgctgcgct catggcctgc acgtccaggt aagggcaagc gcggggattt
      241 tccccgcgca tctgactagc agattcattt tctcatggca ccccttttac acttcagtta
      301 gtgcatgttt ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc
      361 gtacagtgtg agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcaaaa
      421 aaggaaacaa gcgctcctat aaccaagatg agtgccagtt ttttcatata acctccatgg
      481 tttatcgaat cttatgttcg atttgaacgt tatacgctgg tttattttga taccgttgat
      541 gagatcaaag atcgggtgaa ttgattaaag ataagccaga tggacgtatg gataaggatt
      601 atggttttat ttgtcataca aataaatata ataggcgctt ccgatgtaga cccgtattct
      661 tcgcctgtac cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg
      721 tttacgcggc atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc
      781 agccgcagcg tgcaacccga tgacaaaata tcccagattc gccagcattt catgccacga
      841 ctttaagcta tcaacccgtt cgaaattggc ctctgatgca taaggcatcg tcaaaccaaa
      901 cgcaaaccac gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat
      961 aaacaggaga taaatcacca aatggcccaa atgcgccagt cccgtcatca tcggttttgg
     1021 cttaggtata atcggcgggg ttgggtattt cagccttaac aaaagacgaa cgaccatcag
     1081 cacgagaatt gatatgccac aggaaacatg aatcatgttg atgagtggcc gatcgctacg
     1141 tgggaagaaa ccccgaaact ccattgcgca atacgctgcg ataaccagca taaagaccag
     1201 ccagtgaatg ctgatttgta accttgagta tttatttccc ataacatttc ctgctttaac
     1261 ataattttcc gttaacataa cgggcttttc ttaaaatttc attaaatatt gttcacccgt
     1321 tttcagtacg ttacgtttca cggcaagaat gtagattgcc catcccgcca tcctggtcta
     1381 agcttggaaa ggatcaattt tcatccgaac gttcctgaca ggagaagacc atgagtaaag
     1441 ttggtattaa cggttttggt cgtatcggtc gactggtgtt gcgtcgatta cttgaagtca
     1501 aaagcaacat agacattgtc gctattaatg atctcacttc cccaaaaatt ctcgcctacc
     1561 tgctgaaaca tgattcaaac tacggaccgt tcccctggag cgttgatttt acggaagatt
     1621 cacttatcgt tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc
     1681 cgtggaaagc gaaaggggca gaaattattg tcgaatgtac tggcttttat acctccgccg
     1741 agaaatcgca ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gcccctgccg
     1801 gtgaaatgaa aactatcgtt tataacgtca atgacgacac tctggatggc aacgacacca
     1861 ttgtttccgt ggcgtcatgc accactaact gtcttgcacc gatggccaaa gccttgcacg
     1921 acagtttcgg aatagaagtc ggcacgatga cgaccattca tgcctatacc ggcactcagt
     1981 cactggtgga tggtccgcga ggtaaagatc tacgcgcttc acgtgcagcg gcagaaaata
     2041 tcattcccca cactacaggt gcggcaaaag ccattggtct ggtgatcccg gaactgagcg
     2101 gcaaactgaa aggtcatgcg caacgcgtac ccgtgaaaac aggttcggtg actgaactgg
     2161 tgtctattct cgaaaaaaaa gtgactgccg aagaggtgaa caacgcgctt aaaaaggcga
     2221 caaacaataa cgagtcattt ggttataccg atgaagaaat agtctcttct gatatcattg
     2281 gcagccattt cgggtcggtg tttgatgcca cgcaaaccga aattaccgcc gtgggcgatt
     2341 tacagctggt gaaaacggtc gcctggtatg ataacgaata tggcttcgtc acgcagctta
     2401 ttcgcaccct cgaaaaattc gctaaactct gacgcgcaaa ggcggagaga aaagcctccg
     2461 cctctttcac tcattacgac tgtaaataaa ccacctgggt ctgcaggtat tcatgcaagc
     2521 catgtttgcc atctgcgccg ccaataccgg atttacgcca tccggcgtgg aatccttgca
     2581 tagcttcgaa gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt
     2641 tcatcgcgac gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat
     2701 tagccattgc gatagcctct tccagcgtgt caaatgcgac cactggcagc accgggccaa
     2761 aggtttcctc gtgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat
     2821 aataatatcc tttcccctct accgctttgc cacccaacgc cactctcacc ccttcttcta
     2881 ctgcgcgcgc cactttttgc tcgacccttt ccagcgccgc ggcgttaatc aacggcccca
     2941 tcgcaatgtc gttgcgttca gcggggttac caaattgcac cgcctgcatc gcttcaccca
     3001 gccgattgac aaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac
     3061 acacttgccc actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat
     3121 cggcatcgtc cattacgata gccggtgctt taccccccag ttccaggcac actttggtga
     3181 tgtttttcgc cgcagtcgcc ataatctttt cacctgcaga gacgctgcct gtcatactga
     3241 ccattgcgac ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagaa
     3301 caaggttaaa cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg
     3361 cattgtttgg cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg
     3421 gagccatttt gcgggcaatg aggaagaacg ggaagttcca cggcagaatg ccggtagtca
     3481 caccaagcgc acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct
     3541 cgccctcgta acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca
     3601 cttcgacttc agctagctgc tggatcttac ccccttcttc aacaatcagc gtactgattt
     3661 cactggcgcg tttgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag
     3721 caggcaacgc ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat
     3781 cctcggcctg accatcggga atgcgggaaa tgacagcctc tgtagcaggg tttaccacat
     3841 caatccatgc gtctccacgc caggtaacaa actgtccatc aatatacata ggatgttgaa
     3901 cgggtactga catgagcgac tcctgtgatt tatatgtttg tttttggtaa atgcattgtt
     3961 aattaacatc aatcatcata gagacggcat catgaaagga caattctgtg aagggcttca
     4021 taaaaacaag tgtaaaagga ataagaggca gaaataaaag gcaggagtta tgcccgtttg
     4081 caaacaagtg gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg
     4141 atgagcaccg catcgtgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca
     4201 acgtgaacaa taaaatctcg cccgcgggga ccatagccat cgctatcatc gtgtaaacgc
     4261 ggcagctcgc cagtgagtag tgatagataa cgctcaaccg gccataatcc ttgcaacgga
     4321 ttaacaaaga ttacactgtc tgcgttgttt cctagctgag gaacgaatcc gggataactt
     4381 aaccagcgag gtgcatctgg attgtccccg gtcatacggc ggaacgtcgc catcgtgcgc
     4441 cgctgcatgg tggggtcctg aacaacgata gccgtatgaa ctcgttctac ggcctgattc
     4501 aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag
     4561 attttttcat gcggaatatg ccagaactga tgagcgatat ccgccaggat ggtcgcttct
     4621 gctctgccag tggtgcgaat agtgttgtag tgcggatgct gtgcgatggc gctatacaaa
     4681 aaagttgtcg agtgaccgat accaccactg atcagtaaag gaatttgctg atcgcgagca
     4741 atcttacatg ccgcgtcgat agtcggcata accgcattgc ctgcaaggat cacgcaatcg
     4801 gcctgatacg gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt
     4861 atggcatcta tggttgccgg cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct
     4921 tatcgttccc ttaagaatac tgcgtgagat aataatgaga agcggagcac tctgaaagga
     4981 agatgatgct ttttctacct cgccatcgcg aaccagacac tggttttagt catactgtat
     5041 ttgcggagaa actgttgata aaacatcaat aacatcattt gacgtggcaa tgacataatg
     5101 ctcgatcaca ttatttttat aaatttcaca actttgcata gtaaaccatg caaagtaggc
     5161 ggaattcact actctataga tccccgtttg gttaataccg tgaattcctg acattcctaa
     5221 caaatcacct tcgtcagtaa cacgaaatga atgtatccag tcaaatgata acttaacttc
     5281 tgacttgatc gccggacata tgagggtaag ttcaagcctg gcatttttat agcttaaatc
     5341 tgatagttcg gcatttttaa aagacaatga agtcggagtg atatttataa aacattcatc
     5401 catgttaata ccccttcata tatgagactg ctctcgatag acgacacatt aaccttctgc
     5461 aaaaacggtg cgagtctgtt tacgtaccat gtataatcga tggacattac tgtgatttga
     5521 aattaccttg tccagtcatc aaccattttt ttcaggggga acatctgcaa tacattcatt
     5581 aatgcgaaat ataattcccc ctgtggcacc catattctaa tcttctcatc ccctaaaatg
     5641 aaaaattgca attgacgttt gaaacctaat gttttatctt caggtaattc agcttgtatt
     5701 tttttattga tatcatcaag gtagatgtga tgacaatact catcaaacat actcattact
     5761 tccagaccgt ttacagatgt ccatctttgt tcactttccc aataaacaag aa
//
LOCUS       DARNVM010000233         5708 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.233, whole genome shotgun sequence.
ACCESSION   DARNVM010000233 DARNVM010000000
VERSION     DARNVM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.233"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            49..1038
                     /gene="ldhA"
                     /locus_tag="SI621_004439"
     CDS             49..1038
                     /gene="ldhA"
                     /locus_tag="SI621_004439"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEI4067069.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1149..1571
                     /gene="hslJ"
                     /locus_tag="SI621_004440"
     CDS             1149..1571
                     /gene="hslJ"
                     /locus_tag="SI621_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HEI4067070.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGKLTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1568..1834)
                     /locus_tag="SI621_004441"
     CDS             complement(1568..1834)
                     /locus_tag="SI621_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEI4067071.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2108..5632
                     /gene="nifJ"
                     /locus_tag="SI621_004442"
     CDS             2108..5632
                     /gene="nifJ"
                     /locus_tag="SI621_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEI4067072.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELIPQAEIDAHRARGLNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMVERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKTARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDTNGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEIRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1422 a         1471 c         1475 g         1340 t
ORIGIN      
        1 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
       61 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
      121 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac cgccaatggc
      181 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
      241 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gttttaataa cgtcgacctt
      301 gacgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaagcc
      361 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca tcgcgcgtat
      421 cagcgtactc gtgacgccaa cttctctctg gaaggtctga ccggctttac tatgtatggc
      481 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
      541 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcggcggc actggaactc
      601 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
      661 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
      721 aatggtgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcagcaatt
      781 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc
      841 gatctgttct ttgaagataa atccaacgac gtaattcagg atgacgtatt ccgtcgcctg
      901 tctgcctgcc acaacgtgct gtttaccggg catcaggcat tcctgacagc agaagctctg
      961 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg cgaaacctgc
     1021 ccgaacgaac tggtttaatc ttgccgctcc cctgcattcc aggggagctg attcagataa
     1081 tccccaatga cctttcatcc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     1141 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     1201 taagtaatga caaaattgct gtaaccccag aacagctaca gcatcatcgc tttgtgctgg
     1261 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     1321 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     1381 atggtaaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
     1441 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
     1501 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctagcggatt
     1561 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
     1621 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
     1681 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
     1741 gcgctacgtg cgccgcagtc gcctgctgaa caggttcact actacacgcc gacaacaata
     1801 aagcggcaca ccctacccaa aacgctgctc gcatcttttc ttcctctgat cttcaagcca
     1861 aacgacaccg ccataaataa taggcagcac agagggcgac gtcgagagct gtcctgcgcg
     1921 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
     1981 cattacgctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
     2041 tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
     2101 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
     2161 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
     2221 ctgggccgga aacggcttga aaaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
     2281 gtcggaagcg ggggctatcg ctaccgtgca tggcgctttg cagacgggtg ctctttcaac
     2341 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg
     2401 cgaactcaca ccgtttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactctc
     2461 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
     2521 tgcggcaaac gtccaggaag cgcaagactt tgctctcatt tcgcacatcg cgacgctgaa
     2581 gagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
     2641 aattgtcccg ctggccgatg acacgattct tgaactgata ccgcaggcag aaattgatgc
     2701 ccaccgcgcc cgcgggctga atccggagca tccggtgatt cgcggtacct ctgctaaccc
     2761 ggacacctac ttccagtcac gtgaagccac caacccgtgg tacaacgcag tttacgacca
     2821 tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt cgtcagtatc agccgtttga
     2881 gtattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
     2941 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagtcggcg tgctgaaagt
     3001 tcgcctgtac cgtcccttct ccgccaaaca cttgctacaa gctctgccgg gatccgtacg
     3061 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
     3121 ggatgtaatg accgcactgg cagaagcctt taataatggc gagcgcgaaa ctctgccccg
     3181 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
     3241 ggtatttgcc gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga
     3301 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact
     3361 ggaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcgacca aaaacaatat
     3421 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
     3481 aaaggcgggc ggcctgacag tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
     3541 ttatctcatt tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgacaaata
     3601 tcagatggtt gagcgtttaa aacctggcgg cattttcctg ctcaacacgc cgtacagcgc
     3661 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgctaaacc agaaaacagc
     3721 gcgcttctat gtgattaacg cagcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
     3781 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
     3841 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggcc aggatctggt
     3901 ggaacgcaac tggcaggctc tggcactggc gcgtgaatcc gtagaagaag ttccgttgca
     3961 gccggttaat ccgcacagcg ccaatcgacc gcctgtggtt tccgatgccg ctcctgattt
     4021 cgtgaaaacc gttaccgcgg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc
     4081 gctgccgcca gacggcacct ggccgatggg caccacacgc tgggaaaaac gcaatatcgc
     4141 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc
     4201 ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc
     4261 tgccagcctg cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
     4321 gcaggtggca ccggaagatt gcaccggctg taacctgtgc gtcgaagttt gcccggcgaa
     4381 agaccgtcag aatccagaga ttaaagctat caatatgatg tcgcgcctgg aacatgtcga
     4441 agaagagaaa atcaattacg atttcttcct caacctccca gaaatcgacc gtagcaaact
     4501 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attcaggtgc
     4561 ttgctccggt tgtggcgaga cgccgtatat taaattgctg actcagctgt atggcgaccg
     4621 gatgttgatc gctaatgcca ctggctgttc ttcaatatac ggcggtaacc tgccctctac
     4681 accgtatacc accgatacca acggtcgcgg gccggcatgg gcgaactcgc tgtttgaaga
     4741 taacgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgagtgct
     4801 gcgtctgctc gatcaatttg ccgataaaat cccggcagaa ttactgactg cgttgaaatc
     4861 agacgccacg ccagagattc gtcgagaaca ggtcgcagct ttacgccagc aactcaacga
     4921 tgttgccgaa gcacatgaac tgctacgtga tgcagatgca ctggtggaaa aatcaatctg
     4981 gctgattggt ggtgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
     5041 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaataccgg
     5101 tggtcaggcg tcaaaagcga caccgctggg tgcagtaact aagtttggcg agcacggcaa
     5161 acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg tacggccatg tttatgtggc
     5221 gcagatttct ctcggcgcgc agctgaacca gacggtgaaa gcaattcagg aagcggaagc
     5281 gtatccggga ccatcgctga tcatcgctta tagcccgtgt gaagagcatg gttacgatct
     5341 ggctctcagc cacgaccaga tgcgccaact cactgctacc ggcttctggc cgctatatcg
     5401 cttcgatcct cgtcgtgccg atgaaggcaa actgccgctg gcgctggatt cacgcccgcc
     5461 gtcagaagca ctggaagaaa cgttacttca tgagcaacgt ttccgtcgtc tgaattcgca
     5521 gcagccagaa gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta
     5581 tgacttcctg gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgcactg
     5641 gataaggatt atccaattct aaaaaaaagc ccggacgact gttcgggctt gtctcattat
     5701 ttgttgga
//
LOCUS       DARNVM010000234         5669 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.234, whole genome shotgun sequence.
ACCESSION   DARNVM010000234 DARNVM010000000
VERSION     DARNVM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.234"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            113..679
                     /gene="mepS"
                     /locus_tag="SI621_004443"
     CDS             113..679
                     /gene="mepS"
                     /locus_tag="SI621_004443"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HEI4067073.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            860..2401
                     /locus_tag="SI621_004444"
     CDS             860..2401
                     /locus_tag="SI621_004444"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HEI4067074.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQSLRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSEHGVNFMQGYWISRPLPLDDFVR
                     WLKKP"
     gene            2498..4312
                     /locus_tag="SI621_004445"
     CDS             2498..4312
                     /locus_tag="SI621_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEI4067075.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            4313..5407
                     /locus_tag="SI621_004446"
     CDS             4313..5407
                     /locus_tag="SI621_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEI4067076.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGASGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5407..>5669
                     /locus_tag="SI621_004447"
     CDS             5407..>5669
                     /locus_tag="SI621_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416684.1"
                     /note="part of the microcin C transport system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4067077.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRL"
BASE COUNT         1360 a         1396 c         1427 g         1486 t
ORIGIN      
        1 agatttggcc ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc
       61 atttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa
      121 atctcaaccg attttgagat atatcttgcg cgggatcccc gcgattgcag tagcggttct
      181 gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga cacgtgcagt
      241 gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc tggttcgtaa
      301 tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg tacgctatcg
      361 tctgggcggc agcactaaaa aaggtatcga ttgttctggt ttcgtacagc gtacattccg
      421 tgagcaattt ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa tgggtaaatc
      481 tgtttcccgc agtaatttgc gtacgggtga tttagttctg ttccgtgccg gttcaacggg
      541 acgccatgtc ggtatttata tcggcaacaa tcagtttgtc catgcttcca ccagcagtgg
      601 tgttattatt tccagcatga atgaaccgta ctggaagaag cgttacaacg aagcacgccg
      661 ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt
      721 aactgaaagc actgcttagg cagtgctttt ttgttttcat tcatcagaga aaatgatgtt
      781 tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt tgtcgtaatt
      841 tcaggaaatt aacggaatca tgttcatacg cgctcccaat tctggacgta agctcctgct
      901 tacctgcatt gttgcaggcg tgatgattgc gatactggtg agctgccttc agtttttagt
      961 ggcctggcat aagcacgaag tcaaatacga cacactgatt accgacgtac aaaagtatct
     1021 cgatacctat tttgccgacc tgaaatccac tactgaccgg cttcagccgc tgaccttaga
     1081 tacctgccag caggctaacc ccgaactgac cgcccgcgcg gcgtttagca tgaatgtccg
     1141 tacgtttgtg ctggtgaaag ataaaaaaac attctgttca tctgcgaccg gtgagatgga
     1201 cattccactc aatgaattga ttccggcgct cgacattaat aaaaacgtcg atatggcgat
     1261 cttacccggc acgccgatgg tgccgaacaa acccgcaatc gtcatttggt atcgcaaccc
     1321 gttgttgaaa aatagtggcg tgtttgccgc gctgaatctc aatctgacgc cttcactctt
     1381 ctatagctca cggcaggaag attacgatgg tgtcgccctc attattggta atactgcgct
     1441 atctaccttt tcgtcacgtt tgatgaatgt taatgaatta accgacatgc cggtccgtga
     1501 aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcagatgact ggacatggaa
     1561 cgatgtgtgg tacgcatttt tactgggcgg catgagtgga actgtcgttg gcctgctctg
     1621 ctattacctg atgagcgtac gtatgcgccc cggcagagaa atcatgaccg ccatcaagcg
     1681 cgaacaattt tacgtggcgt atcaaccggt ggtggataca caatctttgc gagtaacggg
     1741 cctggaagta ctactacgct ggcggcatcc tgtcgcggga gaaatacccc cggatgcctt
     1801 cattaacttt gccgaatcgc aaaagatgat tgtgccactg actcagcacc tgtttgagtt
     1861 gattgcccgc gatgccgcag aattagaaaa agtactgccg gtaggcgtca aatttggcat
     1921 taacattgcg ccggaccatc tgcacagcga aagctttaaa gctgatatcc agaaactgct
     1981 cacttccctg cccgctcacc atttccagat tgtgctggaa attaccgagc gcgatatgct
     2041 gaaagagcaa gaagccacgc aactcttcgc ctggctgcac tcggtcggcg tagaaattgc
     2101 tattgatgac tttggcaccg ggcacagcgc gcttatctat cttgagcgtt ttacgctcga
     2161 ttatctgaaa attgaccgtg gatttatcaa cgccatcggt acggaaacga tcacttcacc
     2221 cgtacttgac gcggtgctga cgctggcgaa acgtctcaat atgctgacgg ttgctgaagg
     2281 ggtcgaaacg ccggaacagg cgcgatggct aagcgaacac ggcgttaatt tcatgcaagg
     2341 ctactggatt agtcgcccgt taccgctgga cgattttgtc cgctggctga agaaaccgta
     2401 aacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa gcatgccgga
     2461 ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca tactgctgct
     2521 gtttatcgct ctgttcacct ttggtgcgca ggcgcaggct atcaaggaaa gctatgcctt
     2581 tgccgtgctg ggcgaacccc ggtacgcctt taatttcaac cattttgatt atgtgaaccc
     2641 cgccgcgcca aaaggtgggc agataacgtt gtcagccctc ggcaccttcg ataatttcaa
     2701 ccgctatgca ctgcgcggca acccgggcgc acgcaccgag cagctgtacg acacgctatt
     2761 tacgacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa gcgcacgcta
     2821 tgctgacgat tattcctggg tggaggtcgc tattaatccg cgcgcccgtt ttcatgatgg
     2881 ttctcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga ccgaaggcgt
     2941 gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcgc cgttaaccgt
     3001 gcgcattgag ttagctaaac ccggcaaaga agatatgctg agtctgtttt cgctgccggt
     3061 atttccagaa aagtactgga agaatcacaa acttagcgac ccgctcgcca cgcctccgct
     3121 tgccagtggt ccgtaccgaa ttacgtcctg gaaaatgggg caaaatattg tctattcccg
     3181 cgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga atttcgacac
     3241 cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta aagccggtgc
     3301 ctttgatttg cgtatggaaa acgacgccaa aaactgggcc acgcgctata ccggtaaaaa
     3361 tttcgataag aaatacatca tcaaagatga gcaaaagaac gaatcagccc aggatacgcg
     3421 ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtgc gggaagcgat
     3481 cactctcgcc tttgactttg aatggatgaa caaagcgttg ttttacaatg cctggagtcg
     3541 cacgaacagt tattttcaga ataccgaata cgcggccaga aattaccccg acgccgcgga
     3601 gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca cgcaaatcta
     3661 ccagccgccg gtatccaaag gcgatggcta cgatcgtgac aacctgttaa aagccgacaa
     3721 acttctcaac gaagcgggct gggtgctgaa gggtcagcaa cgcgtaaatg ccacaacggg
     3781 tcagccgctc agctttgagt tattgctacc ttcgagcagc aatagtcagt gggtattgcc
     3841 gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaaag tggataactc
     3901 gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcc tatggcgggc
     3961 gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata tcaattccac
     4021 ttataatgcc cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc aaattattgc
     4081 cgcgcaggga aataaagaaa aattactgcc gttggggcga gcactggatc gcgtattaac
     4141 gtggaattat tacatgctgc caatgtggta tatggcggaa gaccgtctcg cctggtggga
     4201 taaattctct caacccgcag tgcgccctgt ttacagcctg ggtatcgata cctggtggta
     4261 tgacgttaat aaagcggcca aactgccgtc agccagacaa cagggagagt agatgggcgc
     4321 ttatctgatt cgccgtctgt tgctggtgat cccaacgtta tgggcgatta tcactatcaa
     4381 ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggctatcg ccgccattga
     4441 gtttggtaat gccggagtat tacccggcgc aagcggtgaa ggtgttcgcg ccagccatgc
     4501 gcaaacgggc gtcggcaata tcagcgacag taattaccgt ggcggacgag gattagatcc
     4561 agaagtgatc gccgagatca ctcatcgcta cggtttcgat aagccgatcc acgaacgtta
     4621 cttcaaaatg ctctgggact acatccgctt tgattttggc gacagccttt ttcgcagcgc
     4681 ctcggtactg acgctgatta aagatagtct gccggtttcc atcaccctcg gattatggag
     4741 cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg tttataatgg
     4801 tagccgcttt gacgtctgga gtagtgcatt tatcatcatc ggctacgcca ttccggcctt
     4861 tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc agctatttcg acctattccc
     4921 tctgcgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga aaatcaccga
     4981 ttatctgtgg catatcacgc tgccggtgct ggcgacagtg attggcggct ttgcggcgct
     5041 gaccatgctg acaaaaaact cattccttga tgaagtgcgt aagcaatacg tggtgactgc
     5101 gcgtgcgaaa ggggtaagtg agaaaaatat tctctggaaa catgtgttcc gcaacgccat
     5161 gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta ccggctcgct
     5221 gctgattgaa gtaatgtttt cgctcaatgg tcttggctta ctgggctacg aagcgaccgt
     5281 ctcgcgcgat tatcctgtaa tgtttggtac tttgtatatt ttcaccctga ttggcctgct
     5341 gctgaatatt gtcagtgata tcagctatac gctggtcgat ccgcgtatag attttgaggg
     5401 acgttaatgt cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt tcgtcataac
     5461 cgtcgcggct actggtcgtt atggattttc ctcgttttgt ttggtttgag tttgtgttct
     5521 gaactgatcg ccaacgataa accgttgctg gtgcgttatg acggcagttg gtatttcccg
     5581 ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagcca ggctgattat
     5641 caggacccgt ggctgaaaca gcggttgga
//
LOCUS       DARNVM010000235         5646 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.235, whole genome shotgun sequence.
ACCESSION   DARNVM010000235 DARNVM010000000
VERSION     DARNVM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.235"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..553
                     /gene="ruvA"
                     /locus_tag="SI621_004448"
     CDS             <1..553
                     /gene="ruvA"
                     /locus_tag="SI621_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEI4067078.1"
                     /translation="VGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYGFN
                     NKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAE
                     RLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRM
                     VSKIARPDASSETLIREALRAAL"
     gene            562..1572
                     /gene="ruvB"
                     /locus_tag="SI621_004449"
     CDS             562..1572
                     /gene="ruvB"
                     /locus_tag="SI621_004449"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEI4067079.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(1722..2507)
                     /gene="znuB"
                     /locus_tag="SI621_004450"
     CDS             complement(1722..2507)
                     /gene="znuB"
                     /locus_tag="SI621_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HEI4067080.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2504..3259)
                     /gene="znuC"
                     /locus_tag="SI621_004451"
     CDS             complement(2504..3259)
                     /gene="znuC"
                     /locus_tag="SI621_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HEI4067081.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3338..4270
                     /gene="znuA"
                     /locus_tag="SI621_004452"
     CDS             3338..4270
                     /gene="znuA"
                     /locus_tag="SI621_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HEI4067082.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            4286..5608
                     /gene="mepM"
                     /locus_tag="SI621_004453"
     CDS             4286..5608
                     /gene="mepM"
                     /locus_tag="SI621_004453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HEI4067083.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
BASE COUNT         1477 a         1434 c         1467 g         1268 t
ORIGIN      
        1 agtgggcggc gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga
       61 agcgggtcag gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct
      121 gtacggtttt aacaataaac aagagcgcac attgttcaaa gagttgatca aaaccaacgg
      181 cgtcggcccg aagttagcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa
      241 tgccgttgag cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac
      301 cgccgaacgc ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt
      361 tacgccagcc gccgacctgg tactcacgtc tcctgccagc ccggcgaccg acgatgctga
      421 acaagaagcg gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat
      481 ggtgagcaaa atcgctcgcc ctgacgccag cagtgaaaca ttaattcgcg aagccctacg
      541 cgccgcgtta tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact
      601 ttgccggaag atgtggcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt
      661 cagccgcagg ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat
      721 gccctcgatc atctgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac
      781 attgtcgcca atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag
      841 gccggcgatc tggcagcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat
      901 gagatccacc gtctatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac
      961 caactggata tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg
     1021 ccgtttaccc tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgat
     1081 cgttttggta ttgtgcagcg tctggagttt tatcaggtgc cggatctgca atatatcgtc
     1141 agtcgcagcg cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct
     1201 cgtcgcgctc gcggtacgcc acgtattgcc aaccgtctgc tgcgtcgagt gcgtgacttc
     1261 gccgaagtga agcacgatgg caccatctcg gctgatatcg ctgctcaggc gctggatatg
     1321 ctgaatgtcg atgccgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc
     1381 gataagttct ttggtgggcc ggtaggtctg gataacctgg cggctgccat tggcgaagaa
     1441 cgtgaaacca ttgaggatgt gctggaacct tatttgattc agcaaggctt tttgcagcgt
     1501 acaccgcgcg ggcgtatggc gacagtgcgg gcgtggaacc acttcggcat cacgccgcca
     1561 gaaatgccgt aagtcggatc ggtcgctcgc ggcgcatccg acactggcag tgttgcctga
     1621 tgcgccgcta ttcgcgtctt atcaggcata taccagagat agcgaaccac aggtcggata
     1681 aggcgctcgc gccgcatccg acaaatgtgt tcagcgaaag attagctggc ctgttttttc
     1741 atcatactga gaataaataa cagtgccgcg catagcacca ccgacgggcc tgctggtgta
     1801 tcgtaaaatg cggaaaaggt taaaccgcca gtcactgcca ccatccccac caaaacagcg
     1861 acacctgcca tctgttccgg cgtgcgggca aagcgacgtg cagtagcagc aggaataatt
     1921 agcaacgaag taataatcaa cgcgccgaca aatttcatcg ctacaccaat cgtcaatgcc
     1981 gtcaccagca tcaacaacaa tttcacgcgc tgtaatttca caccatcaac aaacgccaga
     2041 tccgggctaa tcgtcatcga cagcaaattg cgccattgcc agaacaaaat cgccaccacg
     2101 atgaccacgc caattgcaat agagatgaga tcttctggcg tcactgccag caaatcaccg
     2161 aacaggtaag ccatcaaatc aacacgaata ttagacatca gactaacgac caccaggccc
     2221 agcgacaggg cactgtgcgc cattatccct aataacgtgt cgatcgccag ctgtggacgc
     2281 ttctccagcc ataccagacc gcccgccagc agcagcgtaa ctgcaatcac cgcatagaat
     2341 ggattcacgt ccagcaacaa accaaacgcg acaccaagta atgaggcatg ggccagcgta
     2401 tcaccgaaat aagacatacg acgccagacc acaaacgaac ccagcggacc cgcggcacag
     2461 gcgagcatga tcccggctaa ccaaccggga aataataatt caatcatgag cgatcatttc
     2521 cccgacgcaa aacaattcgt ccctgtaaat cgtgacgatg attatgatga tggcgataga
     2581 tacccagttg ttcagcacca cgaggaccaa acatagaaat aaactccgga tgcagggaaa
     2641 caacttccgg tgtaccggaa caacaaatgt ggtgattcag gcaaagcact tcatcggttt
     2701 ttgccattac cagatgcaga tcgtgagaga ccattaaaac gccacaatcc agttcgcgac
     2761 gcaactggtc aataaggtca tataacgcca cctgaccatt cacatccacg ccctgagtgg
     2821 gttcatccag cactaataat tgcggtcgat ttaacaatgc tcgcgctaac aatacacgct
     2881 gtgtttcgcc acccgagagc ttttgcatcg gtgcgttaat cagatgcccg gcctggacac
     2941 gtttcagtgc aggcaaaata tcttctttat gtgtgccagg gcgtaagcgt aaaaaacggt
     3001 ttacggtaag tggcaacgtg gtgtcgagat acagcttctg cggtacatag ccgatgcgca
     3061 gttttccgtt gcgcttgata accccttcat cgggtgttac cagcccgagc actacccgta
     3121 ccagtgtcga cttacctgcg ccatttggcc caagtaaagt caaaattttt ccaggtttaa
     3181 gttccagcga cacatcagag aggacgcggc gttggccaaa agaaaccgag acattttcca
     3241 gggaaaccag acttgtcatg ttaattttag tcttgcagta gtcatgaaat gttataatat
     3301 cacacttctc atattcatta cgatgattgg tcgcattatg ttacataaaa aaacgcttct
     3361 tttcgcagca ttatccgccg ctctctgggg cggtgcaaca caggccgcag atgctgccgt
     3421 tgtcgcttcg cttaaacccg ttgggttcat cgcttctgcc attgctgatg gggtaacaga
     3481 aacggaggtt ttactacctg acggcgcttc agaacatgat tattcactgc gtccatcaga
     3541 tgtaaaacgc ttacagaacg cggacttagt cgtttgggtt ggcccggaga tggaagcgtt
     3601 catgcaaaaa ccggtaagta aattacctga agcgaaacag gtaacgattg cgcagctaga
     3661 gaatgtgaaa ccgctgctga tgaaaagtat tcacggcgat gatgatgatc acgaccacgc
     3721 ggaaaaaagt gacgaagatc accatcacgg cgatttcaac atgcatcttt ggctttcccc
     3781 agagatagcg cgggctacag cggttgcaat ccatggaaaa ttagtggaac ttatgccgca
     3841 aagtcgagcc aaacttgacg ccaacctgaa ggattttgag gcacaattag cctcaaccga
     3901 aaagcaggtt ggtaacgagc tcgcgccgct caaggggaaa ggttatttcg tttttcacga
     3961 tgcttacggc tatttcgaaa aacagttcgg actgacaccg cttggtcatt ttaccgttaa
     4021 ccctgagatt caacctggcg cgcagcgttt acatgaaata agaacacagt tggttgagca
     4081 aaaagcaacc tgcgtttttg ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc
     4141 acgagggaca tccgttcgta tgggaacgtt ggatcccctg gggacgaata tcaaactggg
     4201 taaaacaagc tattcagaat tcctgaatca attagccaac cagtatgcga gctgcctgaa
     4261 aggagattaa tgaggaagtg attacgtgca acagatagcc cgctctgtcg ccctggcgtt
     4321 taataattta ccgcgaccac accgcgttat gttggggtcg ctcaccgttc ttactctggc
     4381 cgtcgctgtc tggcggccct atgtttatca ccgcgatgcc acgccaattg tcaaaaccat
     4441 tgagctggaa cagaacgaaa ttcgttcgct cttacctgaa gccagtgagc cgattgatca
     4501 agctgcacaa gaagatgaag ccattcccca ggatgaactg gatgacaaaa tcgccggtga
     4561 agcgggcgtg catgaatatg ttgtttccac tggcgatacg ctaagcagca ttctcaatca
     4621 gtatggtatt gatatgggtg atatcaccca actggctgct gccgacaaag aattgcgtaa
     4681 cctgaaaatc ggtcaacaac tctcctggac attaaccgcg gacggcgaac tgcagcgcct
     4741 cacctgggaa gtgtctcgtc gtgaaacccg aacctatgac cgtactgccg ctaacggttt
     4801 taaaatgacc agcgaaatgc agcaaggaga gtgggtcaac aatctgctga aaggtaccgt
     4861 cgggggaagc tttgttgcca gcgccagaaa cgccggttta accagcgccg aagtgagcgc
     4921 agtgattaaa gccatgcagt ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt
     4981 tgcggtgtta atgtcacgag aaatgcttga tggtaaacgt gagcaaagcc aactgctggg
     5041 cgtacgtttg cgttcagaag gtaaagatta ttacgcaatc cgcgctgaag atggcaaatt
     5101 ctatgatcgt aacggtactg gtctggcgaa aggattcttg cgattcccga cggcgaaaca
     5161 gttccgtatc tcgtctaact ttaacccgcg tcgtactaat ccggtgactg gtcgtgttgc
     5221 gccacacaga ggtgttgatt tcgccatgcc acagggtacg ccggtgcttt cagtgggtga
     5281 cggtgaagtg gtggttgcca aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca
     5341 tggtcgcagc tacaccacgc gttatatgca cttgcgcaag attctggtga aaccgggaca
     5401 gaaggtgaaa cgtggcgacc gtatcgcgct ttccggtaat accggacgtt caaccgggcc
     5461 gcatctgcac tatgaagtat ggataaacca gcaggccgta aacccgctga cggcaaaact
     5521 gccgcgtacc gaagggctga ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga
     5581 gattgtgccg cagctacggt ttgattaatt aacatccatt cgcagccggt acgcagtcag
     5641 taccgg
//
LOCUS       DARNVM010000236         5626 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.236, whole genome shotgun sequence.
ACCESSION   DARNVM010000236 DARNVM010000000
VERSION     DARNVM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.236"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            446..835
                     /locus_tag="SI621_004454"
     CDS             446..835
                     /locus_tag="SI621_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067084.1"
                     /translation="MKARIKELEPYRALFIEKPVLAEHDDVIKQSEESETYSCLLRAP
                     AGLFLSFCLLWHYPIFPMLNTDTPEDILSLLGTLWPRAVTVRLPDMPAVQTTGLPGYL
                     FDRVRGQRCEIVRYVTSELFVGARLCK"
     gene            880..1050
                     /locus_tag="SI621_004455"
                     /pseudo
     CDS             880..1050
                     /locus_tag="SI621_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            1097..1231
                     /locus_tag="SI621_004456"
     CDS             1097..1231
                     /locus_tag="SI621_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEI4067085.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRNVLSDNITLKPLEPVGKGNEQ
                     "
     gene            1221..2453
                     /locus_tag="SI621_004457"
     CDS             1221..2453
                     /locus_tag="SI621_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEI4067086.1"
                     /translation="MNSDFSAKKILTGEDVLCAAVHRIEWLFETFSSVCLSFSGGKDS
                     TVLLHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAG
                     NRCGVAILTGVRADESLNRFVGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKAR
                     DIWIYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            2438..3082
                     /locus_tag="SI621_004458"
     CDS             2438..3082
                     /locus_tag="SI621_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEI4067087.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVACINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPAWTVR"
     gene            complement(3453..5600)
                     /locus_tag="SI621_004459"
     CDS             complement(3453..5600)
                     /locus_tag="SI621_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEI4067088.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLHNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEIVKGPYSVLYGSQAIGGIV
                     NFITKKGGNKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVNQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT         1453 a         1190 c         1394 g         1589 t
ORIGIN      
        1 aatatattta ataaattcaa ttatgcaaaa acatgaaaac aagagttcaa atgggtcttt
       61 gataaaagtc aaaaattctc tcttttcttc tgcgtcagat aacatctaag ttatcaactc
      121 ttgtctggcg tcggttactt tacggttatg aattatctta tcctagcgat attgaattga
      181 ttcaaataac aggaaatggt cgtagtacaa aggacagaaa atgatgttga tagaagtgat
      241 ggacagcaca taaaattatc gtagggttgg cggcgatcgt ccggcaaatg ctatcgacgg
      301 tattaaaacc ctgcacgaaa tcggcttcga taccctcaaa ctgaatggtt gtaaagaact
      361 ggggctattc gtgaagcttt tggcaatcag attgagtctg gtcttgattt ccacgattgc
      421 accagcgtgc tgatagtggt tgatagtgaa agcacggatt aaagaactgg aaccgtatcg
      481 cgcgctgttt attgaaaagc cggtgctggc ggagcatgat gatgtgatta agcagtctga
      541 agagtcagaa acttacagtt gtcttttaag agcacctgca ggattgtttc tcagtttctg
      601 cctgctctgg cattacccta tttttcccat gttgaatacg gatacgcctg aggacatttt
      661 gtctctgttg ggcactcttt ggcctcgagc agttactgtt cgcttaccgg atatgcctgc
      721 cgtgcagacc acaggcttac ctggttattt gtttgatcgc gtccggggcc agcgatgcga
      781 aatagtacgc tatgttacca gtgagttatt tgtcggtgcg cgtctttgta aataaagact
      841 ggcgttcatt gttatgcatt gatcattatg gcgaacaatt tacgtttacg cgtacgtgac
      901 tatgtcatca ccctggcggt acccgtgttc gtgaccgcca tgctctgtat ggcagcggtt
      961 atactgtagc gttttatcct gatatggggt ccatcgagtg tgttggctat accgatagaa
     1021 attatcttgt tagctttcgt tctgacataa catcatcaaa ttaaacgaat tctattacac
     1081 aacaaggagt gggaggatga gtaatatcag tttgtattgt ttgccatatt caggtggttc
     1141 tgccgctatg tactataaat ggcgtaacgt gctgtcggac aatattactt tgaagccttt
     1201 agaacctgtg gggaagggga atgaacagtg atttttccgc gaaaaaaatc ctgacgggtg
     1261 aggatgtcct gtgtgcggcc gttcaccgta ttgagtggtt gtttgagaca ttttcttctg
     1321 tctgtttgtc tttttccgga ggaaaagact ctactgtgct gctccatctt acggccgatg
     1381 tggctcgcag gaagaaacgt cgtttctctg tattattcat tgactgggaa gctcagtacc
     1441 agtgcacaat tgcacacatt ctgaagatga gggagatgta tcgggatgtg acggagactt
     1501 tttactgggt ggcactcccc ctgactacgg taaacggtgt ctctcagttt cagccggaat
     1561 ggatatgctg ggaaccaggt gtggagtggg ttcgtcagcc accagatgac gctattacag
     1621 atatgtcgta cttcccattt tatcggtatg ccatgacgtt tgaagaattt gttccggcat
     1681 tttcttcctg gtttgccggt aaccggtgtg gagtggcaat actgactggt gttcgtgctg
     1741 atgaatcgct caatcgcttt gtgggactgg tgtctcagcg caaactgaga tatgcagatg
     1801 ataaaccctg gaccacagcg tcacctgaag ggttttatta caccttgtat ccgttgtatg
     1861 actggaaagc tcgtgatata tggatatata acgccagaac ccgagctatc tacaatcccc
     1921 tgtatgacct gatgtaccgt gccggcgtgc cgttacgcaa catgcgggtc tgtgagcctt
     1981 ttggcccgga acagcgtaag ggactgtggc tttaccatgt tctggagccg gaaacctggg
     2041 ccaggatgtg tgagcgggtg tcgggtgctg ccagcggggc gctttatgcc aatgagagcg
     2101 gtgcctattt tgccctgcgt aagcgtatca cgaagccacc tcatcatacc tggcgtagct
     2161 atgcgatgtt cctgctggat gtgatgccgg aaagaacggc agaacattac cgtaataaaa
     2221 ttgctgtcta cctgcgctgg tatcagacgc ggggcttccc ggatgacatc ccggatgaac
     2281 aggagaatga cctggggagc cgggatatcc cgtcctggcg acgtatctgt aagacactca
     2341 taaagaatga tttctggtgt cggaccctct ccttcagtcc gaacaaaccc cggcactatg
     2401 aacgttatct gcagcgtatg aaagaaagga ggaaggaatg ggggattctg tgacacctga
     2461 ggtggaagta ttgagcaaca tgattcgaca gtacttcagt caggaacgat ctgaagaaga
     2521 gaccatacga gcccttaatc atctgcgcag ggtgttgcat gaggtcagcc cttttgcaca
     2581 ggagcctgtg gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa
     2641 tcccaatgtc atgtcttcca gtgaaaaaaa actgcttaaa cattcactgg agcaggatgg
     2701 attcacgcag cctgtggtgg tttcggaaga gaaggagcat tatctggtag tggatggttt
     2761 tcatcgtcag ttgctgggca ggaaagcgga tacgagaaaa cgtctgaagg gctggctgcc
     2821 agtggcctgt attaatccag agagaaaggg acaggcatca cgtattgccg cgaccatacg
     2881 gcataaccgt gccaggggaa aacaccagat aacgtcaatg tctgacattg tcagggacct
     2941 ttcgcgtctg ggctggacag atgagcgtat tggcacagag ttgggcatgg atcaggatga
     3001 agtgctgagg ctcaaacaga ttagcgggct gacagagctg tttcaggaag agaatttcag
     3061 cccggcatgg acggttcgtt gatattgtaa aatatgatag ttggagtagg gggcaataga
     3121 aggagaatat ttattccacc ttcttccgcg tcgggataaa tcaaaaggta ttatccattg
     3181 attcagtatc tgggggagag taaataatct aaaaatagta ttgcgttgtg aactgatatc
     3241 acttttgaat tacaaaggta taggctcagt agccagcttt ataagtcatt gtcgtagcaa
     3301 tgagtataga atcatgctgg cgggaagaat aaatattcat ctacagatgg aaaacaacga
     3361 cgaaaaacct tcaacaaaca caatacgttc gttagcctgc ccgcaaagcg ggcagatcag
     3421 gacaaagaat tccataacga ttgtttttgt catcagaaat tccatacaaa acctatagct
     3481 gcattaaaac ctgctgcagg aatagtttca tgtgctgtac ggtagcgttt gtctgtcagg
     3541 ttattgagtg ccaggttaat ccggtactga tcctcgttac cgaattctgt atttactgca
     3601 aagttgagag tggcccagcc cggaacattt gtttcggtac cgttactgtc atcttttgca
     3661 ctagaggcag cacgaataaa aacatcagag attatgttgg cctgacccat cacaagagta
     3721 tgtttcagcc ctatacgtcc gtttatcgct ggttcacctg tattagttgt ttttaatgtt
     3781 gaagtttcat attgccgacg aattaaattg ccactgatat atggcgagaa aacccagcca
     3841 ttatattctg cacttatttc cagtccccat gtttttgccc gatcaatatt gtcataataa
     3901 tagtagctac tacgggagga gttggtgtta ccattgcaaa ctatactgcc atcacagatc
     3961 agacttgcaa tataatcttt agcttctgag tagtaaactg cgctgtcaat cagccactga
     4021 ttaccattat atcgtgcacc taattcaaag ttattggagt gttcagcctt aagatcagga
     4081 tttccgtatg tgacactgcc gcccgcagat gtctgcataa aaagctggga gagtgtggga
     4141 aatacgtagc cttgcgcgaa cgcagcgcgt aactccaggt tatcgaaacc tgaatagcgc
     4201 aggcttgtag atgttaccat ttcgtgatca ctcgcagact ctctggcaag agaggtatcg
     4261 cttataatgc ctgcggtata tgatactccg tcaccacgcg tcaactttga agaaagccag
     4321 tattggcgaa ctcccattgt ccatgtccag tgatcggcga attgccagtc attttgtgcg
     4381 aatagtgaga ctgtactttg ctctgactca taataggagc gagttcgtgt ttccttatta
     4441 atgaagccgg tcagagattt gcttgaggtc atgccaccgg acctttggtt gaccctgtct
     4501 tgtttgtact gtgcaccggt aacaagttca ttattagcag gcagcgaaaa gtgactctgc
     4561 aatgtgaccg cctgagtgta ttgcttatca tgggtgtcag tcttgttatg aacggtcaga
     4621 gcctgaatca tcggactggg aacaggctgt gtcgttttta cttcgttggc aaattggcgc
     4681 tggatggtct gctcatacgc gtcgaaatga atttttttta gatagtcacc gtccacgtct
     4741 gtgtcataga ataccccaac tttctctcgt tcaagtttag gtattttgac actaaatgcc
     4801 tcataacttc cgtctggatc ctcatagtaa gtttgtgtcg cgagtctgta gcgatcaagc
     4861 gagaggccaa aacgatggtt tccggagtta taacccaacc atacaccctg actattgtta
     4921 cgatagttgg tattcggcag acgtccatcc ggcgtatcac gattgccctg atcagaataa
     4981 ctaccgttga tgcgataatc aaatccaccg atgctcccct ggaccgcgat tgattcttcc
     5041 cagcctgctg ttgcggaatt ataaacagct ttcacaactc cagatgcaag tttgttacct
     5101 ccctttttgg tgatgaagtt aacaataccg ccaattgcct gtgaaccgta cagtacggaa
     5161 tatggacctt tcactatctc aacacgctcc agcgcagact catctatcaa cagtcccaca
     5221 ccataattat ctccggcgcg ctgataagtt acctcctgac catcaatgag aattaaaaca
     5281 cgggaggatg cttcgccacg aatgcggatt tgtttacggc ctgccaagga gttgtctgtt
     5341 atctctactc cggggatgtc ctgcaaatta tcggctatgg aggcattggt ggagttatgc
     5401 aatgtttgtt ggtcaataac ctgtatagtc gccgggcttt cccaaaggtt gcttgccgaa
     5461 cgattcgtgg tcacgacgag cgtctcttcg ttgctgattt ttgtttctga agaagttgcg
     5521 gttgcagtag aaactatccc agaaatgcat aatgacagag tggaaaggat ataaattcga
     5581 caaggtatat tatataacat ttcatccctc attaatacaa agtaca
//
LOCUS       DARNVM010000237         5495 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.237, whole genome shotgun sequence.
ACCESSION   DARNVM010000237 DARNVM010000000
VERSION     DARNVM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.237"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..140)
                     /gene="yaiA"
                     /locus_tag="SI621_004460"
     CDS             complement(<1..140)
                     /gene="yaiA"
                     /locus_tag="SI621_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HEI4067089.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTA"
     gene            complement(190..714)
                     /gene="aroL"
                     /locus_tag="SI621_004461"
     CDS             complement(190..714)
                     /gene="aroL"
                     /locus_tag="SI621_004461"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HEI4067090.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(897..1355)
                     /locus_tag="SI621_004462"
     CDS             complement(897..1355)
                     /locus_tag="SI621_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEI4067091.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1475..2284
                     /gene="proC"
                     /locus_tag="SI621_004463"
     CDS             1475..2284
                     /gene="proC"
                     /locus_tag="SI621_004463"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEI4067092.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGVMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2301..3416)
                     /gene="adrA"
                     /locus_tag="SI621_004464"
                     /gene_synonym="dgcC"
     CDS             complement(2301..3416)
                     /gene="adrA"
                     /locus_tag="SI621_004464"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HEI4067093.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVMLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3518..3838)
                     /gene="psiF"
                     /locus_tag="SI621_004465"
     CDS             complement(3518..3838)
                     /gene="psiF"
                     /locus_tag="SI621_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HEI4067094.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3957..5372)
                     /gene="phoA"
                     /locus_tag="SI621_004466"
     CDS             complement(3957..5372)
                     /gene="phoA"
                     /locus_tag="SI621_004466"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEI4067095.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPDNALEKGGRGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
BASE COUNT         1270 a         1478 c         1378 g         1369 t
ORIGIN      
        1 gcggtcggat gttcactgat caacgagtct ggtttaggat gatcggctct gagttggtac
       61 caggttaccg tctgtcctgg ttttcctttt tcaatcgtca ctatatatgc ttcacgagga
      121 taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt aagtataggc
      181 gctcgaaaat caacaattga tcgtctgtgc cagggcactg cgaatttcag aaatcacctg
      241 gctgggttcg tttgttgcgt cgatgataat atgcgcaact tcgcgataaa gcgcatcgcg
      301 ttcttccagc acttcctgaa cttcttcgct cagcggtttc cccgttaagg ttggccgtaa
      361 atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg cacacaaata
      421 aaccacgatc ccgttatttt gcataaagtg acgattaaat tccgtcagga taatgccgcc
      481 gcccgtggcg ataacggtgg atggcgcagt taccgcttcc agcgccgccg tttctctggc
      541 gcggaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat tgagctgtga
      601 ttgcaaccac tggtcggtat cgacaaatcg acggttaagc gaatcggcaa gggccattcc
      661 gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg
      721 ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc gatcatacca
      781 tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtgaa aattttaata
      841 catcccttac tataccaata aattttcaag aatgaagtgt aaataatata ttacattcag
      901 ccacgactac gttgcacttc cagccaccac ttctccagct ccgcggcaaa ggcctggcgg
      961 tcacgttgtg aaaggctatc tggtccgccg gtctggatcc cactggcacg taaggtatcc
     1021 ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg ttcgccgcgc
     1081 ggattaagcg ccgcagcgcc tttctcgatg gcttcagcag ccaaaggtat atctgcggtg
     1141 atcaccaaat cgcccgcttc acactgccgg acaatttcgt tatcggcaac gtcgaaacct
     1201 gccgcgacgc gcagcgtacg aataaatcgc gatggcggca cgcgtaaact ctgatttgct
     1261 accagtacca gcggcatctg catacgttcc gccgcgcgat acaaaatctc tttaattaca
     1321 ttgggacacg cgtcggcatc cacccaaatt gtcataaagt catcctttgt tgggtaatcc
     1381 tctattgtgt cgcgcttttg ccttccggca tagttctgtt tatgcttctg ccagcgatta
     1441 tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg gttttattgg
     1501 ctgcggcaat atgggaaaag ccattctcgg cggtctgatt gccagcggtc aggtgcttcc
     1561 agggcaaatc tgggtatata ccccctcccc ggataaagtc gccgccctgc atgaccagtt
     1621 cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc
     1681 cgccgttaaa ccaggcgtca tgattaaagt gcttagcgaa atcacctcca gcctgaataa
     1741 agactctctg gtcgtttcta ttgcagcagg tgtcacgctc gaccagcttg cccgtgcgct
     1801 gggccatgac cggaaaatta tccgcgccat gccgaacact cccgcgctgg ttaatgccgg
     1861 gatgacctcc gtaacgccaa acgcgctggt aaccccagaa gataccgctg atgtgctgaa
     1921 tattttccgc tgctttggcg aagcggaagt aattgctgag ccgatgatcc acccggtggt
     1981 cggtgtgagc ggttcttcgc cagcctacgt ctttatgttt atcgaagcga tggccgacgc
     2041 cgccgtgctg ggcgggatgc ctcgcgccca ggcgtataaa tttgccgctc aggcggtaat
     2101 gggttccgca aaaatggtgc tggaaacggg agaacatccg ggggcactga aagatatggt
     2161 ctgctcaccg ggaggcacca ccattgaggc ggtacgcgta ctggaagaga aaggcttccg
     2221 tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac tcagtaaatc
     2281 ctgatgactt ttgccggacg tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt
     2341 gctttgtaga gcgccaaatc tgccgatttc aaccactcgc gatagtgact catttgtggg
     2401 ttcagcggcg caacccccac actaatccgt aacatcacct gcggcgtatt cggcaggcgt
     2461 aacgtattta atccttcatg cactcgtaac atggcggtaa tggcgctctc agctggcgta
     2521 ccggacatga ttaccgcaaa ctcatcgccg ccaaaccgac caatcacatc gctaccgcgc
     2581 agggttattt gtaattgtcg ggtaagcgcc acaatcgctt catcgcccac atcatggccc
     2641 caggtatcgt tgatgctctt gaaatggtcg atatcgataa tcagtaatgt cgcatcgcgg
     2701 ttatgccgcc gacagttatc aaattcattg cgtaacatag tttcccaatg acgtcggtta
     2761 tacacgcccg tcataccgtc gcgggtactc atgacctgca acctgcgttt atgttccgcc
     2821 agtttagtcg ccgtctggta gctgacccag ccaaacagca gaggataaat gacgataatg
     2881 ggaagggaga gccaccattc cagcggcgca ctattgaacg acacggtgat gcccgtcagt
     2941 tcaagggtga caagacagga aaccaccatc aataccagac ccgcgacaaa cagacggggg
     3001 ccgcctgccc ccatcaaatt cagacacata atcatcaaca tcgcggtgga aggcagcacg
     3061 tttacgccca ttacgcctac ccacattccc gctaatactg catcggtttt taagttgtaa
     3121 atttcccggc taagcggatc gacggccctg ctcgctatct gccaggctaa atgcggccag
     3181 acgaacgccc agccgaccaa caccagccac caccagcccg gcggcgggtg cgaaaccagc
     3241 gttgaagcaa tcggtaagaa catgccagcc aggccaaccg cgcggggtag tctgacgcga
     3301 cgggcgaagc gcagcccgga tcgctgatgt tcgttttgag gagttaaagg aggttcctcc
     3361 ccatgcgccg ccgctttttt gaaaaagttt tcatcattca ttatttttgg gaacattcag
     3421 aaacaatttt cccaaattat agagacgggg ctaaaggcag aagtatgaaa tttcgggtga
     3481 gccattttcg tagctcaccc gttagcacta tcaggtatca ggcggctttc ttgaggcagg
     3541 cgctcataaa cttattacga tcatcacctt tcagagattg ttgcgttgct tgattattac
     3601 attcgcgcat cttttgctgc tgtggcgtca aacttttttc accaggcgca gacttgctgt
     3661 tcttcaggca atcactcatg taggtcttac gagcatcccc tttcaacgcc tgcgccgtcg
     3721 cctgctgatt acaggaggtc atacgctgtt gttgcggggt taaggttctc tcggcagcgc
     3781 cgacggtggt taaaaaaacc agaccgaaaa gcaaggtaac cagtaatgtt attttcatag
     3841 caccatccct cttcatgttt taaccatgag cgtatgcgcc cgtgatctgc cattaagtct
     3901 ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc actgccgggc gcggttttat
     3961 ttcagcccca gggcggcttt catggtgtag aagagatcgg tctggtcggt tagtccaaca
     4021 acattggcgg catgcgggcc atacgccgca atacgcaact gactgccggt atgttcttgt
     4081 gaatcctctt cggagttccc gtaactcatc accatcactg cgccatcttt ggtatttagt
     4141 gcctgggtga ggcccggcgc tttggtgtcc ggcgcgacaa tctggctggc gtgggcgtga
     4201 tcagcggtga ctatgaccag cgtgttgcca tcctttttag cgaattccag cgcacgttgt
     4261 acggcttcat cgagatcgac cgtctcgcca atttgcccac aaggattcgc agcgtgatcc
     4321 tgtttatcga tggatgcacc ttcaacttgc aggaaaaagc ctttctcatt tttactcaac
     4381 aattcaatgg ctttgtcggt catctgcgcc agggtcggta cgctgtcatt acgttgcgga
     4441 ttaggcgtac aggtaactgc gggcttgtcg atattgccgt ggtacgttgc tttcggtcct
     4501 agccagcgca ctggcatatt gccgtcagca aacaggccaa gcaggggttt ttgctgattc
     4561 gcttccgtca ccgcatttag cgatgcagca tcactcacca gctgataacc acgcgcctgt
     4621 gcctgttcac gcagcgtttt tccctgccat tcgccagcgg ttgccgtttc agcaaaggtt
     4681 tttgcgccgc cgccaagcgt aacatcggca cgggcgttaa gcagctgttc ggtaatcgat
     4741 cctctcccgc ctttttccag agcgttatcc ggacattttt cactggtcgc gctcggaccg
     4801 tagcatttgc gcgaggtcac atgcgccacc agcgcggcgg gcgtggcatc ctgcaactct
     4861 gcggtagaaa cgttaccggt cgccagacct gcggcttttg ccatttccag aatcgttggg
     4921 tgatcttttt cgtgaatatc gacgcccagc gcaccgttat aggttttgac accagttgac
     4981 caggcggttg ctgatgcagc cgagtcggta acgtagtccg gtttgccggt ttttttattc
     5041 agcgcatagt gtgtgtattg tccggtaagc ggtaaggcat cgataccttt aaaaaagccg
     5101 cccgcacctt cggcataatt gcgtgcagca gtaatttccg agtcccccat cccatcgcca
     5161 atcagcaaaa taatattttt ggcaggttta tcgctaagag aatcacgcag agcggcggtc
     5221 tgatcacccg ttaagcggcg agcaccgccg ggtgcagtaa tatctccctg agcagcccgg
     5281 ttttccagaa caggcatttc tggtgtccgg gcttttgtca caggggtaaa cagtaacggt
     5341 aagagtgcca gtgcaatagt gctttgtttc actttatttt ctccatgtac aaatacatta
     5401 aaaaataaaa acaaagcgac tataagtctc ggccgtgaca gctttatgac agctgttgaa
     5461 aagattaact ttttactgac gaggatgctt caata
//
LOCUS       DARNVM010000238         5474 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.238, whole genome shotgun sequence.
ACCESSION   DARNVM010000238 DARNVM010000000
VERSION     DARNVM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.238"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(61..2130)
                     /gene="glyS"
                     /locus_tag="SI621_004467"
     CDS             complement(61..2130)
                     /gene="glyS"
                     /locus_tag="SI621_004467"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEI4067096.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDDADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPAVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2140..3051)
                     /gene="glyQ"
                     /locus_tag="SI621_004468"
     CDS             complement(2140..3051)
                     /gene="glyQ"
                     /locus_tag="SI621_004468"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEI4067097.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3146..3454)
                     /locus_tag="SI621_004469"
     CDS             complement(3146..3454)
                     /locus_tag="SI621_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HEI4067098.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQVSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3629..4624
                     /gene="wecH"
                     /locus_tag="SI621_004470"
     CDS             3629..4624
                     /gene="wecH"
                     /locus_tag="SI621_004470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HEI4067099.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALSTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWGSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4666..5103)
                     /locus_tag="SI621_004471"
     CDS             complement(4666..5103)
                     /locus_tag="SI621_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HEI4067100.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQITQEEYSE"
     gene            complement(5149..5442)
                     /locus_tag="SI621_004472"
     CDS             complement(5149..5442)
                     /locus_tag="SI621_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067101.1"
                     /translation="MVYLVGLWISCPLLSGKGYFLGVLMTATFGNYAYLRAEKLGQLD
                     DFFIHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVCLFGQMRLFRSA"
BASE COUNT         1335 a         1419 c         1337 g         1383 t
ORIGIN      
        1 ctgtattatt tagcgataaa aaaagcctgc cagatggcag gctttttaat aacgacgtta
       61 ttactgcaac agcgaaatat ccgcaactcg caggaacagt tcgcgcagtt tctccagcat
      121 ggtcagacgg ttgagacgca attctttgtc atcaaccatg accatcactt tatcgaagaa
      181 agcatcaact ggctcacgca gctctgccag ttcgaccagc gcatcctggt aacgaccttc
      241 agcaaagtac ggctccagtt tatcgcgcag cacaaccacc tgcatcgcca gtttaatttc
      301 ttccggctct ttcagagtag aagcattcac gcgatcgctc agcacttcgt cagacttcgc
      361 cagaatgtta gatacacgct tgtttgccgc cgccagtgac gctgccgcat ccagagtacg
      421 gaagtgcgat actgccttca tacgggcatc aaaatctgcc ggacgcgtcg gacgacgtgc
      481 cagtaccgcc tggattgtgt caacggtgta accttcgtcc tgataccagg cgcggaagcg
      541 accgagcata aagtcgataa catcatcaac cacgttggca ttagtcagct tatcgccata
      601 cagacgcact gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct tctcaacgat
      661 aatacgcagt acgccaagtg cggcacgacg caacgcaaac gggtctttgt cgcctttcgg
      721 atgctggccg ataccgaaga tacccgccag agtatccatc ttgtcagcaa tcgccagcgc
      781 acaggctacc gggttagacg gcaggtcatc accggcaaag cgcggctgat actgctcgtt
      841 cagcgccact gcaacatctt ccgcttcgcc atcgtgacgc gcgtagtgca tccccataac
      901 gccctgggtg tcggtgaact cgaagaccat gttggtcatc aggtcgcact tagacagcag
      961 gcccgcacgg gtagcatggt taacgtcagc gccaatctgt tcagcaatcc agccagccag
     1021 tgcctggatg cggtcagttt tgtcacgcag tgtacccagc tgttgctgga acagcacggt
     1081 ttgcaggcgc ggcaggttat cttccagacg ttttttacgg tcggtattga agaagaactc
     1141 agcatccgcc agacgtggac gaacgacttt ctcgttaccg gagataatct gctgcggatc
     1201 tttcgattcg atgttggcaa cgaagataaa gttcggcaga agtttgccgt cgttcgcata
     1261 caccgggaag tacttctggt cacctttcat ggtgtaaacc agcgcttcag acggcaccgc
     1321 gaggaatttc tcttcgaatt ttgcggtcag cacagccggc cattccacca gcgaagcgac
     1381 ttcttccaac aggctttcgc ttaagtcagc gttaccgcca atcttacgcg ccgcttcttc
     1441 agcatcggct ttaatcttcg ctttacgttc ttcgtaatcg gcgatgactt taccgcgctc
     1501 acgcagaatt tccggatact gatcggcatc gtcgatagtg aattccggct cgcccataaa
     1561 gcggtggccg cgaatcacgc gatcggactg aatgcccaga atggtagccg gaatgacttt
     1621 gtcgcccagc agcagggtca cggtgtgcac cggacgcacg aagtgcacgt cgcttgcgcc
     1681 ccagcgcatc agtttcggga tcggcagttt cgccagcgaa gtcgcaacca tattcggcag
     1741 cagtgcttcg gtgctttcgc ccttcacgtg ggcgcgatac agcagccatt cacctttatc
     1801 ggtagtcaga cgctcagcct ggtctacggt aataccgcaa ccacgcgccc agccttctgc
     1861 cgctttgctc ggtttgcctt cagcgtcgaa cgcctgagca atcgccgggc cgcgtttttc
     1921 gatttcacga tccggttgcg cttccgccag gttagctact ttcagcgcca gacgacgcgg
     1981 agcagcaaac cattgaacgg tgccgtgtgc gaggccggcg ttatccagct ccgcagtgaa
     2041 gttcgcagca aaggactcag ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc
     2101 gatttccacc agaaaagttt tctcagacat agccgcctct tacttatctt tgttgcacat
     2161 cgggaagccg agggcttcac gggaagcgta gtatgcttca gccactgctt tggtcagggt
     2221 gcgaattcgc agaatgtagc gctgacgctc ggtgacggag atggctttac gcgcatccag
     2281 caggttgaag ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg gcagcggatt
     2341 ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc tcaaagcagg tgaacaggaa
     2401 gtccacatcc gcgtattcga agttgtaagt ggactgctcc acttcgttct gatggaacac
     2461 gtcgccgtag gtggttttac ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc
     2521 tacgccctga atgtacattg ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg
     2581 tttacactcc agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt
     2641 cagccacact tcccagccca gcccccaggc acccagcgtc gggttttccc agttatcttc
     2701 cacgaaacgg atgtcgtgga tggtcgggtc catgcccagc tctttcagag aaccaaggta
     2761 cagctcctga atattgtccg gcgatggctt aatgaccacc tggaactgat agtagtgctg
     2821 taaacggttg gggttttcgc cgtagcgacc atcggtcggg cgacgagaag gctgaacata
     2881 agcagccgcc atcggttctg gccccagcgc gcgcagacag gtcattgggt gagaggttcc
     2941 cgcgccgact tccatgtcca atggttgaac aatggtgcag ccctggcgag cccagtaatc
     3001 ctgtaaggtc aggatcaagc cctggaaggt cctggtatca aacttttgca tattatttcg
     3061 tgctggatac gtgtggattt aaaggaaggg atcagtatac ccgctggatg gaagatatac
     3121 agtacgaaac gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa tggccatctg
     3181 cgtcaaaaga acagacaaac tgctctttac ggaaggtata accgcgcatt tcatagctta
     3241 cctgaaattg ttcaaaagca gtgacgtcga ttttttgctc gccggtgtta taacgttttg
     3301 ccgcctgatc cttgcataac gcttccatat tcaacgaacg cagaggatca acctttaccc
     3361 gttgtgcttt ctgcacgggt ggcggtgtag aacaacccac tagcgccata agcaccataa
     3421 gcgccattgc cggaaagaaa gagttcatca tcattaaatt accgtctggt ttgcaggtga
     3481 gtcttattat ttatatcggt atagtggctg atatcttacg aatttcctta agcctctata
     3541 agctgtagtg ataaaactca gattattcca gtgcgccaac cgaatgattt atcggtcaga
     3601 ctttttttaa ccagatacag agacacagat gcagcccaaa atttactgga ttgataacct
     3661 gcgagggata gcgtgtttaa tggtggtgat gattcacacc actacctggt atgtgaccaa
     3721 tgctcatagt gttagccccg tcacctggga tatcgccaat gttctgaatt ctgcctctcg
     3781 tgtcagcgtg ccgctatttt tcatgatttc cggctatctc ttttttggcg aacgcagcgc
     3841 ccagccgcgc cattttttac gtatcggctt atgtctgctt ttttatagcg caatcgcact
     3901 gctctacatt gcgctgtcta cctccatcaa tgtggagtta gcgctgaaaa acctgctgca
     3961 aaagccagtg ttttaccact tgtggttttt cttcgcgatt gcggtgattt atctggtttc
     4021 accgctgatt caggtgaaga acgtcggcgg aaaaatgttg ctggtgctaa tggtggtgat
     4081 tggtattatc gctaacccaa acacagtgcc gcagaaaatt gacggttttg aatggctgcc
     4141 aattaactta tatatcaatg gcgatacttt ttactacatc ctttatggca tgttgggccg
     4201 cgctataggg atgatggaca cacagcataa agcactgtcg tgggggagcg ccgcactgtt
     4261 tgcgacgggg gtttttatta tctctcgcgg gacattatat gaattgcagt ggcgcggaaa
     4321 ttttgccgat acctggtatc tttactgtgg gccgatggtt tttatctgcg caatcacgct
     4381 attgactctg gttaaaaaca cgcttgatac gcgtaccgtt cgcggacttg gcttaatctc
     4441 ccgccattcg ttgggtatat acggattcca cgccttgatt atccatgcac tgcgcacccg
     4501 ggggattgag cttaaaaact ggccaatact cgatattatt tggatctttt gcgcgacgtt
     4561 ggcagcgagt ttgttacttt ctatgctggt acaacgaatc gacagaaaca gactagtgag
     4621 ttaaataaaa gcccggtcac attggactga ccgggcttat gtgaattatt cgctgtattc
     4681 ttcctgggta atctgtgttt cttttggtgg gtttattccg gcatcacgaa tattcttctg
     4741 caccgctact gcaccaaaca agcttaagaa gaaagccagt ccataaaaac ctttctcgct
     4801 gagtaataac gtcgcattcc acagacctac catcagtaac gcaacagaga taataaagac
     4861 agtcaggcag gtcatataat aaatggaggt ggtcggtatg ccttcatact tgtcccgaac
     4921 ggtcttttga taagacgccg cagaaaacag tcctaatacc agtacggcaa aataatatcc
     4981 tttttcattt aactgcatct ctgcattcca tagccctaac agataggtaa cgataccacc
     5041 aacgagagct atccatgaca caatactgaa tgccggagaa taggttgata ttttgttgtc
     5101 catcacagta ttcctttcat tcctgaatgt gtaagaggtt tatgttgctc atgccgagcg
     5161 aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg atgggataaa tcaccatttc
     5221 ataagcattg ataggtgcgt ttaaaacacc gataaacaag agaccgatag tgatcaacgc
     5281 aactaactgg cagatatgga taaaaaaatc atccagttgc ccgagttttt ctgcgcgaag
     5341 atatgcatag ttaccaaaag ttgctgtcat taacacgcca agaaaatagc cttttccact
     5401 taacaatggg catgagatcc atagcccaac cagataaacc agcgccccaa caacaaataa
     5461 tagttttgca acag
//
LOCUS       DARNVM010000239         5469 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.239, whole genome shotgun sequence.
ACCESSION   DARNVM010000239 DARNVM010000000
VERSION     DARNVM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.239"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..110
                     /locus_tag="SI621_004473"
     CDS             <1..110
                     /locus_tag="SI621_004473"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEI4067102.1"
                     /translation="LRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            182..1540
                     /gene="mltD"
                     /locus_tag="SI621_004474"
     CDS             182..1540
                     /gene="mltD"
                     /locus_tag="SI621_004474"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HEI4067103.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1587..2357)
                     /locus_tag="SI621_004475"
     CDS             complement(1587..2357)
                     /locus_tag="SI621_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEI4067104.1"
                     /translation="MTSQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIIVEAHKAA"
     gene            complement(2435..3235)
                     /locus_tag="SI621_004476"
     CDS             complement(2435..3235)
                     /locus_tag="SI621_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEI4067105.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3476..4390
                     /locus_tag="SI621_004477"
     CDS             3476..4390
                     /locus_tag="SI621_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4067106.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4387..5190)
                     /gene="dkgB"
                     /locus_tag="SI621_004478"
     CDS             complement(4387..5190)
                     /gene="dkgB"
                     /locus_tag="SI621_004478"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HEI4067107.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLQESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5352..5428)
                     /locus_tag="SI621_004479"
     tRNA            complement(5352..5428)
                     /locus_tag="SI621_004479"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5392..5394),aa:Asp,seq:gtc)
BASE COUNT         1442 a         1312 c         1357 g         1358 t
ORIGIN      
        1 ttttaagaac agaagatatt gatttaatta atgtaattaa tgaagaaaca ttattgcaac
       61 aacctgaaga acgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc
      121 ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca
      181 catgaaggca aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagtaccgg
      241 caacgttcaa cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt
      301 tacaagtcag gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg
      361 ggctttcatt ggcgacgagc taaagatggg aattccggaa aatgaccgga ttcgcgaaca
      421 gaaacagaaa tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc
      481 gtatatgtac tggatagccg ggcaagttaa aaaacgtaac atgcctatgg aactggtact
      541 actacccata gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgcagcagg
      601 catctggcag atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta
      661 tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg ctgaacatga tgcagcgtct
      721 gaacaagatg tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg
      781 tcgagtcatg aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc
      841 gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat
      901 tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct
      961 ggcgcgtgtg cacctgagca gcccagttga aatggcgaag gttgcagata tggcggggat
     1021 ttccgtcagc aagcttaaga cattcaacgc tggcgtgaaa ggctccacgc tgggcgcaag
     1081 tggtccgcag tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg aatctctggc
     1141 ttcgggcgaa attgctgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag
     1201 ccgtgtttac accgtacgct ctggcgacac gctttcaagt atcgcttcac gtctcggcgt
     1261 tagcaccaaa gatttgcagc agtggaacaa actgcgcgga tctaagctga agccaggcca
     1321 aagcttgacg attggcgcag gtagtagcgc acagcggttg gcaaacaaca gcgatagcat
     1381 tacatatcgt gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa
     1441 catcaaagat gtgatgcgct ggaacagcga tactgcgaat ctgcaaccag gcgataagct
     1501 gacgttgttt gtgaaaaaca acaacatgcc agattcctga caaatcagat aatgaaaggc
     1561 accgattccc ccggtgcctt ttttatttat gctgctttat gtgcttctac aatgatgata
     1621 tcactggtga aagagccatc attctgcaag gcaaaatacg ttttcacctc tgtcgatgcg
     1681 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcgcgacc
     1741 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     1801 tcattgatta acgtcaacca ctcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     1861 aatgcttcta ccgtctgtaa ccagacgtcg cgcactgggt gccccggaga cattacgtcc
     1921 atcacaatca gcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcacca
     1981 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     2041 aatggcagac tttcgacata accctggcgg gtggtgatat ttttcagttg ccgggcttcg
     2101 gcagcttgtg ccacgacatc cagcatttgg gcagataagt catacgccac caccgcgctc
     2161 acgttctgcg cagcgacaaa gctggcatgc cctgctccgc agcccatatc aagtacactt
     2221 gcatcaggat aatcagccag acgcaccgcc aggcgctgta aatcccgtcc ggatgcatgt
     2281 acggtactag tcagatattc gctagcttga gagctaaact gcttttctac gtggtcatgg
     2341 tgggattgtg atgtcattgc gctgtccttt tgttgtttga gtataaaagg gcagcgccaa
     2401 acgttggtct gccctgtggc agacctgaca taccttattt atcaggcttg ccgggactga
     2461 attcaacgag tagcggattg tgatcggaag cgcgcgtaac cagtacagaa gcttcgctga
     2521 cgttcagacc acggtaaaaa acaaaatcga gcgggcgacc aaacgcccga cggcgctgat
     2581 cgtcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat aacgcgttca
     2641 tccttctacg gctccaggca ttgaaatctc ccgccataat gaccgggccg ctgtgatgag
     2701 ctatctgatc gccaatagga agtaactgct tactataaac atccacgccc agactgaagt
     2761 tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga tagaccgtca
     2821 ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg cagcaataca
     2881 ctggatgtgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagc acgaaagcgg
     2941 gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg tactaactct ggcgttgtct
     3001 gcgcttcctg caataacacc agatgtgcat ctttgccgta gttctttaat accgacaacc
     3061 attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc tcttcggtac
     3121 ttaacggttc tccgggtggt aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga
     3181 tcctttccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt cgcacttttg
     3241 actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac tgttataggg
     3301 attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag tgcttacacc
     3361 ggtcagaaat ttgctcatta ttgagaacat taaatcggat gacatatcac gcatcctgat
     3421 ggtttaatga caaataaatt caaaatatct catctatttt gcaacaggag caaaaatgaa
     3481 agccacgtcg gaagaactcg ccatttttgt ttcggtcgtc gaaagcggca gctttagccg
     3541 ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcggtga agaagctgga
     3601 gatgaaactt ggcgttagcc tgcttaatcg gaccacgcgg caacttagcc tgacggaaga
     3661 aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcag cggcagaatc
     3721 agaaattatg gagacgcgta atacaccgcg tggactgttg cgcatcgatg ccgcaactcc
     3781 agtagtgctg cactttctga tgccgttgat taagcctttc cgtgaacgct atccggaagt
     3841 cactttatca ctagtctcct ccgaaacgat tattaatttg atcgaaagaa aagtggatgt
     3901 cgcgatacgc gccggtacgt taacggattc cagcttacgt gccaggccgt tatttaacag
     3961 ttatcgaaaa attatagcct cccccgatta tatttcccgc tatgggaagc cagaaacgat
     4021 cgacgattta aagcaacatg tttgcctggg attcactgaa cccgcttccc tcaatacctg
     4081 gccgatagcc tgtagcgatg gacaattaca tgaggtgaag tacggtttgt catccaatag
     4141 tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt tgtccgacta
     4201 catgatcgac agagaaatcg ctcgcggaga attggtggag ctaatggcag ataaagtgtt
     4261 gccagtggaa atgccattca gtgcagtcta ttacagcgac cgtgcggtaa gtacgcgcat
     4321 ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag gagctgtcag
     4381 agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt cgttgcaatc
     4441 cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg ccttaagatt
     4501 actttccagg tttttacgtt tagtagaaga aggaattact gagtaacctt cccccatagc
     4561 ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcgatac gagcaataac
     4621 ctcatctttc agggccttac catacgccag cgtcatatag gaagtaatat ggatgccgtg
     4681 ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt caatctggtt
     4741 agtagcgatg ttttcagcac caacagcagc aatcgctttt tccatcaatg ggatcgtgaa
     4801 gttggaaata ccgatctcac gcgtcagccc ttgttttttg gcttccagca gcgcctgcat
     4861 aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg tcagatcaac
     4921 ataatcagta cgcaattttt gcaggctctc ttgcagactc gggatcaatt tgtctttgct
     4981 gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc
     5041 tgcaatcgcc tgacctacgg cagcttcgtt atcatagatt tgtgcggtat caattgcgcg
     5101 ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt tgagacggaa
     5161 agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta
     5221 actgaggtga ttatgggctt cagactgttg cggaaaaaga gcgtaaaatg cagaggattt
     5281 ttgcgattct ggcaataata gatatacaga cgaaaaaaac ctcgagcgtt aactcgaggc
     5341 ttcttaataa gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
     5401 gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc ggttttatga
     5461 ccggcttac
//
LOCUS       DARNVM010000240         5449 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.240, whole genome shotgun sequence.
ACCESSION   DARNVM010000240 DARNVM010000000
VERSION     DARNVM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.240"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..178)
                     /locus_tag="SI621_004480"
     CDS             complement(<1..178)
                     /locus_tag="SI621_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEI4067108.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQ"
     gene            377..1075
                     /locus_tag="SI621_004481"
     CDS             377..1075
                     /locus_tag="SI621_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEI4067109.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQIYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1120..2019)
                     /gene="eamA"
                     /locus_tag="SI621_004482"
     CDS             complement(1120..2019)
                     /gene="eamA"
                     /locus_tag="SI621_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HEI4067110.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2214..3401
                     /gene="ydeE"
                     /locus_tag="SI621_004483"
     CDS             2214..3401
                     /gene="ydeE"
                     /locus_tag="SI621_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HEI4067111.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETVSVWTPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3528..3623
                     /gene="mgtS"
                     /locus_tag="SI621_004484"
     CDS             3528..3623
                     /gene="mgtS"
                     /locus_tag="SI621_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HEI4067112.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3821..4732)
                     /gene="dgcZ"
                     /locus_tag="SI621_004485"
     CDS             complement(3821..4732)
                     /gene="dgcZ"
                     /locus_tag="SI621_004485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HEI4067113.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRVSAPFNIH"
     gene            complement(4987..5379)
                     /gene="ydeI"
                     /locus_tag="SI621_004486"
     CDS             complement(4987..5379)
                     /gene="ydeI"
                     /locus_tag="SI621_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HEI4067114.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKKMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1502 a         1296 c         1133 g         1518 t
ORIGIN      
        1 cttgcgggcc aagtaaaaca acgtcaactt cgttattttt aattttccct ttagcctctg
       61 caatcgccaa tgcggaaata ttaacttcaa gtgaaatagc gttagcatga tcaatcatac
      121 gtttaaccag catactggtt gacatgcccg cagcgcaaac taacaatatc tttttcatat
      181 tttcccttac tggtatataa cagattacat tgtgtaaata tcatatcccg cagtagctat
      241 acgtcctggt acattgatca cagtaataca gcactaaagt tgttcaacat cagggtgata
      301 attaaacgac tcggccatac gataaaccaa tcaggactca actccttgtt ttcaatggct
      361 cttaaaaggt gcaaaagtga tttttcaaaa gattgcccgc ttacttaaat ccgagatcaa
      421 tggctattca tggcatgttg gtgatttgtt gccgtcagaa gctgaactcg ctgttcgtta
      481 taatgtttcg cgtaataccc tgcgtaaggc attatccttg ctggaaggcg aaggaattat
      541 tcatagaaag catggttcag gaacatatat tcaaaaaaag aattttgttg cacacattga
      601 tcacatgaac agcttcagtg aaattgcgca taaaagcggc aaggaggcag gaagtcagat
      661 tatgaaattt gaagtgcagg atgcttctcc ccctattgcg actgagctga atttagtgac
      721 tggtgagcaa atttattaca taaagagact gcgattcatt gaggataatg cagcacaatt
      781 agaagaaacg tggatgtcag tggcacgttt tcctgattta accgtagcac atatgcaaaa
      841 atccaaattt tcgtatattg agaacgaatg cgggatcaaa atcattggca cctttgaaac
      901 tttctcccct acttttccta ccccagaaat cgccagtatt ttacggatca gcccacggga
      961 tcccatactt aaaatccaga cccaggctgt ggacagtaac tctattccgc tggattattc
     1021 gttactttac agcaatattt tcgagttcca ggtaaagtac tttttcccgc gataaaagca
     1081 gacgcacaga gtgctgacat cacattctgt gcgtatggtt tagcctctaa cctttaccgc
     1141 tttacgccac cgcaagccaa atacattgat atacagcccg gtcataatga gcaccgcccc
     1201 gaaaaattgt agacccgtta agcgttcatc caacaatagt gccgcacttg ccagtcctac
     1261 tacgggcacc agtaacgata acggtgcaac ccgccaggtt tcataacgtc ccagtaacgt
     1321 cccccagatc ccataaccaa caattgtcgc cacaaacgcc agatacatca gagacaagat
     1381 ggtggtcata tcgatagtaa ccagactgtg aatcatggtt gcggaaccat cgagaatcag
     1441 cgaggcaaca aagaagggaa tgattgggat taaagcgctc cagattacca gcgacatcac
     1501 cgccggacgc gttgagtgcg acatgatctt tttattgaag atgttgccac acgcccaact
     1561 aaatgctgcc gccagggtca acataaagcc gagcatcgcc acatgctgac cgttcagact
     1621 atcttcgatt aacaccagta cgccaaaaat cgctaaggcg atccccgcca attgtttgcc
     1681 atgcagtcgc tcaccgaaag taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg
     1741 taacaccagc gaagccagtc cagcaggcat accgaagtta atggcacaaa aaagaaaagc
     1801 aaactgcgca aaactgatgg ttaatccata ccccagcagc aaattcagcg gtactttcgg
     1861 tcgtgcgaca aaaaagatag ccggaaaagc aaccagcata aagcgcaaac cggccaacat
     1921 cagcggtggc atgttatgaa gccccacttt gatgaccaca aaatttagcc cccatacgac
     1981 cactaccagt agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat
     2041 cttttgtaag caatcaactt agctgaattt acttttcttt aacagttgat tcgttagtcg
     2101 ccgattacga cggcattaat gcgcaaataa gtcgctatac ttcggatttt tgccatgcta
     2161 tttctttaca tctctaaaac aaaacataac gaaacgcact gctggacaga ctaatgaact
     2221 tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta accatcggac
     2281 gcggcgctac gctgccgttt atgaccattt acttgagtcg ccagtacaac ctgagtgtcg
     2341 atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt agtctcggtt
     2401 ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca attaccgcct
     2461 tcgccagcgg ttttattgcc attcctttag tgaataacgt gacgctggtg gtgctctttt
     2521 ttgccctcat taactgcgcc tattccgttt ttgctaccgt gttgaaagcc tggtttgccg
     2581 acaatctttc ctccaccagc aaaacgaaaa tcttctcaat caactacacc atgctcaaca
     2641 ttggctggac catcggtccg ccgctcggca cgctgttggt gatgcagagc atcaatctgc
     2701 ccttttggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa atttgggtaa
     2761 agcgcagcga gaaaatcatc gccacggaaa cagtcagtgt ctggacgccg aaagttttat
     2821 tacaagataa agcactgttg tggtttacct gctctggttt tctggcttcc tttgttagcg
     2881 gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggt gattttgccg
     2941 aaaaggtggt tgcggttgtt cttccggtta atgctgccat ggtagttacg ttgcaatatt
     3001 ccgtgggacg ccgacttaac ccggctaaca tccgcgcgct gatgacagca ggcaccctct
     3061 gtttcgtcat cggtctggtt ggttttattt tttccggcaa cagcctgcta ttgtggggta
     3121 tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag tatatgttga
     3181 ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttccgctcag tctttaggct
     3241 ggcttggtgc ggcgattaac ccattagtga gtggcatagt gcttaccagt ctgccgcctt
     3301 tctcgctgtt tatcatctta gcgttggtga tcattgctgc gtgggtgctg atgttaaaag
     3361 gaattcgtgc aagaccgtgg gggcagcccg cgctttgttg atttaagtcg aacacaataa
     3421 agatttaatt catccttcgt ttaggttacc tctgctaata tctttctcat tgagatgaaa
     3481 attaaggtaa gcgaggaaac acaccacacc ataaacggag ggaaataatg ctgggtaata
     3541 tgaatgtttt tatggccgta ctgggaataa ttttattttc tggttttctg gccgcgtatt
     3601 tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc ggccccttgt
     3661 tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg
     3721 gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag gcattttcct
     3781 gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg ttaaacggag
     3841 ctgaaactcg gttaatcaca ttttgttcgt caataaacat gcagcgattt cttccggttt
     3901 gcttaccctc atacattgcc cggtccgctc ttccaatgac cacatccaga ggctcttcag
     3961 gaaatgcgcg actcacacct gcagtcacgg taatgttgat atgcccttca gaatgtgtga
     4021 tggcatggtt atcgactaac tggcaaattc tgatacctgc acgacatgct tcttcatcat
     4081 tagccgcttt gacaataatg ataaattctt cgccaccgta gcgataaacc gtttcgtaat
     4141 cgcgcgtcca actggctaag taagttgcca gggtgcgtaa tactacatcg ccgattaaat
     4201 gcccgtaggt atcattaacc aatttaaatc ggtcaatatc caacaacatt aaataaagat
     4261 tcagaggctc agcgttgcgt aactgatgat caaaggattc atcaagaacc cgacgacccg
     4321 gcaatcccgt caaaacatcc atattgctac ggatcgtcag caaataaatt ttgtaatcgg
     4381 ttaatgccgc agtaaaagaa agcaaccctt cctgaaaggc gtcgaaatgc gcgtcctgcc
     4441 agtgattttc aacaatagcc aacattaatt cccgaccaca gttatgcata tgttgatggg
     4501 cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggtccc agatgatcaa
     4561 tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct ggcttactgg
     4621 catctctcgc gaccacgctg tgaaacatac tcaccagcca ctggtagtgg gcatcgatag
     4681 ccttattgag atttaacaag atggcatcaa tttccgttgt cttcttgatc attgccactc
     4741 ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat tacgtcaata
     4801 ttttcataga aatccgaagt tatgagtcat ctctgagata acattgtgat ttaaaacaaa
     4861 atcagcggat aaaaaagtgt ttaattctgt aaatcacctc tgcattatcg taaataaaag
     4921 gatgacaaat agcataaccc aataccctaa tggcccagta gttcaggcca tcaggctaat
     4981 ttatttttat ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt tcttatccag
     5041 actgccgcta atgttgatca tctggtccgg ctgaacttct cgtccatcaa agacggccgc
     5101 aggaataacg acattaattt caccgctctt atcgcgaaaa acgtaacggt cctctccttt
     5161 gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca tcgtgcattt tttttgcgaa
     5221 atcaacggtc attttttttg catcatcggt tccgcgatag ccatcttcta ttgcatgagg
     5281 cggcggtggc gctgcatcct gttttaatcc gccctggtca tctgccaacg cataaggcat
     5341 gacaagaaaa cttgctaata caatggcctg aaatttcata ctgactcctt aattgcgttt
     5401 ggtttgactt attaagtctg gttgctattt ttataattga caaataaga
//
LOCUS       DARNVM010000241         5424 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.241, whole genome shotgun sequence.
ACCESSION   DARNVM010000241 DARNVM010000000
VERSION     DARNVM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.241"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..508
                     /locus_tag="SI621_004487"
     CDS             <1..508
                     /locus_tag="SI621_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEI4067115.1"
                     /translation="SAIIVYGMFALGLWMVTLSYYDGWYHKAPELHKSIGILLMMGLV
                     IRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLFAIGISGYLISTADGKPISVF
                     GWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHGFMALKHHFIDKDDTLKRMLG
                     KSSSDYGV"
     gene            512..1087
                     /locus_tag="SI621_004488"
     CDS             512..1087
                     /locus_tag="SI621_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEI4067116.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PLATFTSTSVKKDGDDLDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1129..2181)
                     /locus_tag="SI621_004489"
     CDS             complement(1129..2181)
                     /locus_tag="SI621_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEI4067117.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2406..3326
                     /gene="lpxL"
                     /locus_tag="SI621_004490"
     CDS             2406..3326
                     /gene="lpxL"
                     /locus_tag="SI621_004490"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HEI4067118.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3498..4724
                     /gene="mdtG"
                     /locus_tag="SI621_004491"
     CDS             3498..4724
                     /gene="mdtG"
                     /locus_tag="SI621_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HEI4067119.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4807..5181
                     /locus_tag="SI621_004492"
     CDS             4807..5181
                     /locus_tag="SI621_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HEI4067120.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWGEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5182..5409)
                     /locus_tag="SI621_004493"
     CDS             complement(5182..5409)
                     /locus_tag="SI621_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HEI4067121.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT         1317 a         1377 c         1355 g         1375 t
ORIGIN      
        1 aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta
       61 ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat
      121 gatggggctg gttattcgcg ttctgtggcg cgtcatatct ccaccgccgg gtccgctgca
      181 gagttattcg cccatgactc gtcttggcgc aaaggctgga catctcgcat tgtacttatt
      241 gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag
      301 cgtttttggc tggtttgacg tccccgcgac cctcgccgac gctggcgcac aggctgactt
      361 cgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtccg ttatgcatgg
      421 atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc gcatgctggg
      481 aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt
      541 cgcttccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga
      601 aggtcagcac gcctttgtta acttccgcat ccagcatctt ggctatagct ggttatatgg
      661 cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg ctgccgataa
      721 agtgaatgtg acaattaaca ccaccagcgt cgatactaat cacgccgaac gcgataaaca
      781 tcttcgcagt gcagatttcc tcaatactgc aaaatatcca ctggcaacat tcacctccac
      841 cagcgtgaag aaagacggtg acgacctgga tattaccggc gatctgacgc tgaatggcgt
      901 aaccaaaccc gtcacgctgg aagcgaaatt aattggtcaa ggcgacgacc cgtggggtgg
      961 taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc aaggacttca atatcaaaac
     1021 agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag gcgtacagca
     1081 gaagtaagcg tgaaatgttg ccggatggca cgcatccggc aatgatattt attctgttgg
     1141 atcaggaatg cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc
     1201 attttcacgt cctgcccgac ggcgtcgctg ttcttcaggc ggtaacgcgc tttcttcgca
     1261 gcaaatctca ctacaacaac ctttgtattt ttccgcacat actggacact gaataaaaag
     1321 caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcac cgcactggtg
     1381 gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa aaacaaaatt
     1441 tttgccaata aaacgtaccg gtaaaccctg ctcgcgcgcc ttgcgggcgt attcaataat
     1501 tccgccctcg atatgccaga ctttattgaa tccgttatgt ttcatccagg cactggcctt
     1561 ttcacaacga atgccgccgg tgcagtacat aacgattttt ttatctttat gtgcctgcat
     1621 catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt ccagcgcgtt
     1681 ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaaca atgcatcggg
     1741 atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcacccacat tgctggcatc
     1801 aaagtgagga tcgtcaatac cgtcggcaac gatacgatcg cggaccttca tgcgcagtac
     1861 ccagaaggat ttcccgtcat catccaacgc gatattcagg cgtaagccct ctaaagccgg
     1921 gtcgaaggca taaagctgcg cgcgaaatgt ttcaacattg ctcgcaggta cgctgatttg
     1981 cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acatccagcg cggtaaacag
     2041 ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact tgtaaaacga
     2101 aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt cgttggaaat
     2161 gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc
     2221 ggcatgatat agcaattatc gataattaac atccacacat tttacgctac atttgcgcat
     2281 taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc cacaaatttt
     2341 ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gtataggttg aaaaacagga
     2401 ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa
     2461 cctggttggg tattggcgta ctttggttag tcgtgcaatt gccctacccg gttatctacc
     2521 gcctcggttg tggattagga aaacttgcgt tacgttttat gaaacgacgc gcaaaaattg
     2581 tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg
     2641 tggtgaagaa tttcgaatcc gttggcatgg gactgatgga aactggcatg gcgtggttct
     2701 ggccggaccg cagaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg
     2761 tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg
     2821 gtgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc
     2881 cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc
     2941 gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac
     3001 cggatcatga ttacggcccg cgctcaagcg ttttcgtccc gttgtttgcc gttgagcagg
     3061 ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct
     3121 tcgttccacg ccgtaagcca gatggcaaag ggtatcagtt gattatgctg ccgccagagt
     3181 gttctccgcc gctggatgat gccgaaacca ccgccgcgtg gatgaacaaa gtggtcgaaa
     3241 aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt aaaacacgcc
     3301 cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga agggtatccg
     3361 gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg
     3421 gaagtcgggc gcataattag tgtgcttatc ttttcttctt atgttcaccg cgcctggcgc
     3481 accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa
     3541 acctgatcgt cgcctggcta ggctgttttc ttacgggtgc cgccttcagc ctggtaatgc
     3601 ccttcttacc cctctacgtt gagcagcttg gcgttacggg ccactccgcc ttgaatatgt
     3661 ggtccggtat tgtcttcagc attacatttt tattttctgc catcgcctca ccgttttggg
     3721 gtggactcgc cgaccgtaaa ggccgcaaac tcatgctatt acgctctgcc ctcggcatgg
     3781 gcatcgtgat ggtgttgatg gggctggcac aaaatatctg gcagtttttg atcctacggg
     3841 cgcttcttgg gttacttggc ggatttgtcc ccaacgctaa tgctcttatc gccacacaag
     3901 taccgcgtaa taaaagcggc tgggcgctgg gtacgctctc cacaggcggc gttagtggtg
     3961 cgttgctcgg cccaatggct ggcggcctgc tcgccgatag ctacggctta cgtccggtat
     4021 tctttattac cgccagtgtg ctcatactct gctttttcgt caccctgttt tgcatcagag
     4081 aaaaattcca gccggtcagc aaaaaagaga tgctgcacat gcgtgaagtg gtgacatcac
     4141 ttaaaaaccc gaaactggta ctcagcctgt ttgtcactac gttaatcatc caggtggcaa
     4201 cgggctcaat tgcccccatt ctgacgctgt atgtccgcga actggcgggt aacgtcagta
     4261 acgtcgcctt tatcagtggc atgatcgcct cggtgccagg cgtggcggct ctgctaagtg
     4321 caccacgact cggcaaactt ggcgatcgaa tcggacccga aaagatcctg attacggcgc
     4381 tgatcttttc tgtactgctg ttgatcccaa tgtcttacgt tcagacgcca ttacaacttg
     4441 ggattttacg ttttttgctc ggtgccgccg atggtgcact actccccgcc gtacagacac
     4501 tgttggttta caactcgagc aaccaaatcg ccgggcgtat cttcagctat aaccagtcgt
     4561 ttcgtgatat tggaaacgtt accggaccat tgatgggagc cgcgatttca gcgaactacg
     4621 gtttcagagc ggtatttctc gtcaccgctg gcgtagtgtt attcaacgca gtctattcat
     4681 ggaacagtct acgtcgtcgt cgaatacccc aggtatcgaa ctgatttttc gcctttcata
     4741 cttgtaaaag cggagaatca gctatccttt tccctgaaac ctcatcaact caaagggaga
     4801 atcgtgatga ccatgtacgc aacgcttgaa gaagccattg acgctgcacg cgaagaattt
     4861 cttgcagaca accccggcat cgacgccgaa gatgcgaatg tgcaacagtt caatgcccaa
     4921 aaatacgttt tgcaggacgg cgacatcatg tggcaagttg agttttttgc cgacgaaggg
     4981 gaagaaggtg aatgtttacc tatgcttagc ggtgaagccg cgcaaagtgt ctttgatggc
     5041 gactatgatg agatagagat ccgccaggag tggcaggaag agaatacatt acatgaatgg
     5101 ggcgaggggg aatttcagct tgagccaccg ctggataccg aggaaggacg cgcagcagct
     5161 gatgagtggg atgaacgtta attactcata cgggccatga tgaatgtcga tcggcagcag
     5221 taaagtatca aaaaccaggg agaatggcag atcaaggatt gtgacataac gccaggcgga
     5281 gtcacgcaca tcccattgca caccgggata atattggttg ccatgcccct gccccggtat
     5341 agtgcggcta atgatgctgc cacagccact cagcaaggtc accatgatgc tcaccacaat
     5401 taaacgcatt tgatacctct tccg
//
LOCUS       DARNVM010000242         5414 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.242, whole genome shotgun sequence.
ACCESSION   DARNVM010000242 DARNVM010000000
VERSION     DARNVM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.242"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            71..262
                     /locus_tag="SI621_004494"
     CDS             71..262
                     /locus_tag="SI621_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEI4067122.1"
                     /translation="MLTLPHFQFIYALMGAERILFSVDYPYQTLDGVKTFIDSLPVNK
                     AEKEAIAFRNAERLLGITV"
     gene            413..985
                     /locus_tag="SI621_004495"
     CDS             413..985
                     /locus_tag="SI621_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEI4067123.1"
                     /translation="MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPD
                     GKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKL
                     ILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
                     KIAQQKTLAREHASRLVELLNSVVNNPEGE"
     gene            988..2007
                     /locus_tag="SI621_004496"
     CDS             988..2007
                     /locus_tag="SI621_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEI4067124.1"
                     /translation="MQYTRLGKSDLLVSRICMGCMGFGDPSTGQHSWTLDETASRDII
                     RYGLEKGINFYDTAIAYQNGSSERYVGRALREMAKRDDIVLATKFLPRTPAQIAGGIS
                     GKEAIAQSLDQSLKNLGMDYIDLYIYHIWDYNTPIIDVLEALHTAVTAGKVRAVGISN
                     CYAWQLAKANALAEREGLTPFVSVQSHYNLIMREDERELFGLCTEDNIAMTPYSALAS
                     GRLSRKEGYTRRATEDDYARGKYDSTAEQDRSIIERVAELAERHLVSMTEISLAWLLT
                     KVTSPVVGATKKAHVDGAVNAVNLQLSPEEIQFLEETYQPHVLTGIMAQNTPQTKDVK
                     QVWMR"
     gene            2153..2848
                     /locus_tag="SI621_004497"
     CDS             2153..2848
                     /locus_tag="SI621_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEI4067125.1"
                     /translation="MLISSPVFATKPDAPNKPEMNIFELGIRPDRDNDFADVARQTIS
                     ASVDHEAGTLAMYALQRSDNSGQAFMFELYENERAYRKHLNAAPYKAFAARAPDIIDR
                     KKKITLEPQFLGDKHIIPNERTINNLVIVEVKPEFQSEFRNIVLPEMVESLKVEKGVL
                     AMYAGTDSETPKRWYFYEIYASEEDYQLHRQTLHFLDYLRQTAHMSARKDAIPVKPVF
                     LRNKGGIKQEPQR"
     gene            2864..3667
                     /locus_tag="SI621_004498"
     CDS             2864..3667
                     /locus_tag="SI621_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEI4067126.1"
                     /translation="MKSVLAAAAMSLVISDFANAEETRGKAMMKIEPSAISEADIRSV
                     SPALARFGREAITEDLWTRDALSPRDRSVVTVAMLIARNQPAELKHYIDVALDSGVTP
                     AELSEIITHLAFYSGWPNAMSAVSVTKAVFETRGVTPDALPDASPDLLPLNQEAEKQR
                     SETVEKNVGRISPGLVKFTADPLFLDLWQRPALTPRDRSLITVSALIASGQSAQIGYH
                     LNRAMDNGLSAEEAGEIVTQAAFYAGWPNAFTAAPVVGEVLINRSSSKT"
     gene            4070..4336
                     /locus_tag="SI621_004499"
                     /pseudo
     CDS             4070..4336
                     /locus_tag="SI621_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            4770..5333
                     /gene="fimA"
                     /locus_tag="SI621_004500"
     CDS             4770..5333
                     /gene="fimA"
                     /locus_tag="SI621_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HEI4067127.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         1460 a         1277 c         1284 g         1393 t
ORIGIN      
        1 ctggctgcaa cgggtctgtc acgcacgcta acgagaacct tccaggaaca cgtttacgtt
       61 accccgtcat atgctaacac tgccgcactt ccagtttatc tacgcgttaa tgggtgctga
      121 aaggatcctg ttctccgtcg attatcccta tcagaccctg gacggtgtaa aaacctttat
      181 agacagtctg cccgtcaaca aggctgaaaa agaggccatc gcgtttcgca atgcagaacg
      241 tttactgggc atcacggttt agttcaccgt ttcatgatga ctgagcacat tgtgatcagc
      301 cgacaattat tagtttttgc ctaaaagtga atgagccttt actgctctaa aaaaataatg
      361 ttgtcactct atactggatt taatattcac caaagatgtt taactgattg aaatgaaaaa
      421 tatccttgtt atttcaggtc atcctgagct tacccattcc gtcgccaatg ccactattct
      481 tgatgaagtg gagaccgccc ttcctgatgt tgagattcgt cgtctggaca ggctctatcc
      541 ggatggcaaa ttcaacatcg cggcggagca ggaaagcctg ctcagggccg atgtgattgt
      601 ctggcagttc cctttttcct ggtatgggct gccaggatta atgaaaaaat ggctggacga
      661 ggtctttgtt cacggcttcg cgcatggctc aacggcgaaa ctgggcggta aaaagctgat
      721 cctctccttt acaacgggag cgccagagga gctctatacc gtcgacagtt tctttggcca
      781 taccattgaa gagtatctta ttccgttcga aaccacggca aggctgtgca atcttgagct
      841 gctggcgccg gtttatacct gcggtatcag ctatgccgac cgggatgccg acaaaatcgc
      901 gcagcaaaaa acgcttgccc gggaacacgc ttcaaggctt gtcgaactgc tcaattccgt
      961 cgtgaataat ccagagggag aataacaatg cagtataccc ggctcggtaa aagtgactta
     1021 cttgtttccc gcatctgcat gggatgcatg gggtttggcg atccgtcgac gggtcagcat
     1081 agctggacgc tggacgaaac agcaagccgc gacatcattc gctatggcct tgaaaagggc
     1141 atcaactttt acgataccgc tatagcctac cagaatggct ccagtgagcg atacgttggc
     1201 cgggcattac gggagatggc gaaacgcgat gatatcgtcc tggccaccaa gtttctgccg
     1261 cgaacccccg cacaaattgc cggagggatc agcggaaaag aggcaatcgc tcaatccctt
     1321 gaccagagcc tgaagaatct ggggatggac tatatcgacc tctacatcta ccacatctgg
     1381 gattacaaca cgcccattat cgacgtgctt gaagcgttac ataccgctgt tactgctggc
     1441 aaagtacgag ctgttggtat ttcaaactgc tatgcctggc agctggcgaa ggcgaacgcc
     1501 cttgctgaac gcgaagggct gacgcccttt gtttccgtac agagccacta caatctaatt
     1561 atgcgcgaag atgaacggga gctcttcggc ctgtgcactg aggataatat cgcaatgacc
     1621 ccctatagcg cgctggccag cggccgcctc tctcgtaaag aaggctatac acggcgagcc
     1681 acagaggatg actatgcgcg tggaaaatat gacagcacgg ctgaacagga tcgcagcatt
     1741 attgagcgtg tagccgaact ggccgaacgc catctggtgt caatgacgga aatatctctc
     1801 gcttggctgc tgacaaaagt cacatcacct gtcgtagggg ctacaaaaaa agctcatgtc
     1861 gatggcgcgg taaacgcagt aaatcttcaa ctaagtccgg aggaaattca gtttctggaa
     1921 gaaacctacc agccgcatgt tctgacgggg atcatggcgc aaaatacgcc gcaaacgaaa
     1981 gatgttaagc aggtctggat gcgatagccc ttattcactt ttcctcctct cgtctttgag
     2041 agcgccagta gcggtcggtt acgttcttac cagcaccagt tgatcgaacg tcttaaacag
     2101 taatgtcagt aaaaagatca tcgtgaaaaa gacttttatg cttttaattt gcatgctaat
     2161 aagcagccct gtctttgcaa caaaaccgga cgcgccgaat aagccggaga tgaatatctt
     2221 cgagctcggc atcagacccg atcgggacaa cgactttgca gacgtggcca gacagacgat
     2281 ttccgcttcg gttgatcatg aagcaggtac actggcgatg tatgccctgc aacgcagcga
     2341 caattcaggt caggcattca tgttcgaact ctatgaaaat gagcgtgctt atcgcaaaca
     2401 tctcaatgcc gccccataca aagcattcgc tgctcgtgca cctgacatta ttgacaggaa
     2461 gaagaaaatt accctggagc cacagtttct gggcgacaaa cacatcatac cgaatgaacg
     2521 aactatcaat aatctggtga tcgtcgaagt aaagcctgaa tttcagagcg aatttagaaa
     2581 catcgtcctt cctgaaatgg tcgagtcgct caaagtagag aaaggcgtgt tagccatgta
     2641 tgccggtaca gattcagaga ctccaaaacg ctggtatttc tatgagattt acgccagtga
     2701 ggaggattat caactgcacc gacaaacgct gcacttcctg gattacctca ggcaaacggc
     2761 gcatatgagc gccaggaagg acgcaatccc ggtaaaaccg gtatttcttc gtaacaaagg
     2821 cggaatcaaa caggagccgc aacgttgacc agtaaggatt aacatgaaaa gcgtacttgc
     2881 agcagcggct atgtcgcttg ttatttctga ttttgccaat gctgaggaaa caagaggtaa
     2941 agctatgatg aaaatagaac cgtcagcgat ttcggaagct gatattcgtt ccgtctcacc
     3001 ggctctcgcg cgctttggtc gcgaagctat tactgaagat ctgtggacac gcgacgccct
     3061 ctcaccgagg gatcgtagtg tggtgaccgt cgccatgctg attgcccgca accagcctgc
     3121 cgagcttaag cactatattg acgtcgctct cgatagcggc gtgacgccag cggaattatc
     3181 agaaatcatt acccatctgg cgttctactc aggttggcca aatgcaatgt cagccgtcag
     3241 cgtgaccaaa gcagtatttg aaactcgtgg cgtcacgcca gatgcgcttc ctgacgcttc
     3301 tccggacctg cttcctctca atcaggaagc agaaaagcag cgttcagaga cagtggaaaa
     3361 aaatgttggc cgaatatctc caggcttggt taaatttact gctgacccac tgttcctgga
     3421 tctctggcaa agacccgcgc taaccccccg tgatagaagc ctgattacgg tcagcgcgtt
     3481 aatcgcatct ggacagagcg cgcagattgg ctatcacctt aacagagcga tggataatgg
     3541 actttctgct gaggaagctg gggagatcgt tacccaagcc gcgttttacg ctggctggcc
     3601 aaacgcattt accgctgctc ctgttgtcgg agaggtatta atcaatcgct catcgagtaa
     3661 aacgtaatat tactgaacac caaaacctga cgcacatcac aacattagaa cgaacagttc
     3721 atgaaccaac agacgtgaca tcgagtgatt tatggacagc gtttaacaca gttatgaacc
     3781 aaaactgagt tcataactgt gagtttgaat cgtcatttga ttaatatcca tttaaaacag
     3841 tttgttatgc tgtttctggt gctgaagtca attatgtata tccctcaaac ccagaatagt
     3901 taaatggaat ttgagtgatg cgaagcgatt ttacggtcgt cgttattact tcagggttcc
     3961 agaacataaa gcctctcctc tttcatgatt ggcctgtgca taccggatat aggctggctg
     4021 gtatgcaggt ttgccgtttg ccgcatcttc cctgcgttga agagtcaggc tggcaaaccg
     4081 cgtgcaaatg aacacaccct ctttccatca acacttcaga cagcttgcgg gaatgagtcc
     4141 actccggtat cagaaatggc tacgcctcaa tgaagccagg cgactgatgc tgaatgaaca
     4201 ttacgatgtc accactgcag cctatgctgt cggttacgaa agcttatccc atttcagtcg
     4261 ggaatattcg cggatgtttg gagagtcacc taagagagat attgccaggt taagaaagtc
     4321 tgtcgatcag ctttaataaa ccctgcattt atctggttag agcaatttgg cgttagtttc
     4381 agaaagcaaa cattaaccat agctaatgat ttttagccat ataaaccatt agtaactctt
     4441 gacctttcct gacacttccc ttgttcgcaa taatattctg tttagtcgct gtttataaat
     4501 aacatgcata atgaaatcgt aatgttattt agcgacataa aatgtctttg caaatactct
     4561 ctacaacaag tgctgcaata aacttgttgt gattaggtgt ccttgtaatt taaagatatt
     4621 atcgttcttc gtgaaatgta ataaccctgg aaactatatt ctcagggcaa tatcccatca
     4681 agaatgattc ttgttttata agcaataaca gaatgacttg attgttaact ttctttatta
     4741 agctacttaa atataaattt tggagaatta tgaaactcaa acatgtcggt atgattgtcg
     4801 tttctgtgtt ggcgatgtcg tctgctgcgg taagcgcagc cgagggtgat gaatcagtaa
     4861 cgaccactgt taatggcggt gttattcatt ttaaaggtga agtggtaaat gccgcttgtg
     4921 cgattgattc agaatcaatg aaccaaacgg ttgagctggg tcaggtccgt tcttctcgtc
     4981 tggctaaagc gggtgacctc agctccgccg ttggcttcaa tatcaagctg aatgattgtg
     5041 ataccaatgt ttccagtaat gcagctgttg cattcctggg tactactgtc accagtaatg
     5101 acgatacgtt agcgctgcag agttcagcgg caggctctgc ccaaaatgtc ggtattcaaa
     5161 ttttggaccg tacgggtgag gtattaatac ttgatggtgc cacttttagt gctaaaactg
     5221 acttgattga tggcacgaat atattgccgt tccaggctcg atatattgct ctcgggcagt
     5281 cagtagctgg tactgcaaac gctgatgcga ccttcaaagt tcaatatcta taattcaatc
     5341 ttaaggtggc tatacgtcat ctgaaattga aacagggtgt gtgtggcatg gctacgcaca
     5401 cccgagcaat tcac
//
LOCUS       DARNVM010000243         5277 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.243, whole genome shotgun sequence.
ACCESSION   DARNVM010000243 DARNVM010000000
VERSION     DARNVM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.243"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..894
                     /locus_tag="SI621_004501"
     CDS             <1..894
                     /locus_tag="SI621_004501"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEI4067128.1"
                     /translation="ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTY
                     TGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGED
                     PGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASL
                     KYLSDHPKLQGIAQQNSFKHT"
     gene            1050..2585
                     /gene="gadC"
                     /locus_tag="SI621_004502"
     CDS             1050..2585
                     /gene="gadC"
                     /locus_tag="SI621_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HEI4067129.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSKNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2716..4035
                     /locus_tag="SI621_004503"
     CDS             2716..4035
                     /locus_tag="SI621_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HEI4067130.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNTTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPASRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4333..4374
                     /locus_tag="SI621_004504"
     CDS             4333..4374
                     /locus_tag="SI621_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067131.1"
                     /translation="MVQLVDLARCVSF"
     gene            4412..>5277
                     /gene="dosC"
                     /locus_tag="SI621_004505"
     CDS             4412..>5277
                     /gene="dosC"
                     /locus_tag="SI621_004505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HEI4067132.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFE"
BASE COUNT         1306 a         1284 c         1296 g         1391 t
ORIGIN      
        1 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
       61 gacccgaaac gcatgattga ggcctgcgac gaaaacacca tcggcgtggt gccgactttc
      121 ggcgtgacct acaccggtaa ctatgagttc ccgcaaccgc tgcacgatgc gttggataaa
      181 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgccgccag cggtggcttc
      241 ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg
      301 atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg
      361 cgtgatgaag aagcgctgcc gcaggaactg gtgttcaacg ttgactatct cggtggtcag
      421 attgggactt tcgccatcaa cttctcccgc ccggcgggtc aggtaattgc acagtactat
      481 gaattcctgc gcctcggtcg tgaaggctat accaaagtac agaacgcctc ttaccaggtt
      541 gccgcttatc tggcggatga aatcgccaaa ctggggccgt atgagttcat ctgtaccggt
      601 cgcccggacg aaggcatccc ggcggtttgc ttcaaactga aagagggtga agatccggga
      661 tacaccctct acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc
      721 actctcggcg gtgaagccac tgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc
      781 gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc
      841 gaccacccga aactgcaggg tattgcccag cagaacagct ttaaacatac ctgataacgt
      901 ttaacggtaa cggtgtcccg aaacgaaccc gtttcgggac aatttccaaa gtctgttcac
      961 tggcattagc aacggaaaat attgttctga atacgcttca gaacaaaaca ggtgcggttc
     1021 cgacaggaat accgttttag ggggataata tggctacatc agtacagaca ggtaaagcta
     1081 agcagctcac attactcgga ttctttgcca taacggcatc gatggtaatg gctgtttatg
     1141 aataccctac cttcgcaaca tcgggctttt cattagtctt cttcctgcta ttaggcggga
     1201 ttttatggtt tattcccgtg ggactttgtg ctgcggaaat ggccaccgtc gacggctggg
     1261 aagaaggtgg tgtcttcgcc tgggtatcaa atactctggg gccgaggtgg ggatttgcag
     1321 cgatctcatt tggctatctg caaatcgcca ttggttttat tccgatgctc tatttcgtgt
     1381 taggggcact ctcctacatc ctgaaatggc cagcgctgaa tgaagaccct attaccaaaa
     1441 ctattgcagc actcatcatt ctttgggcgc tggcattaac gcagtttggt ggcacgaaat
     1501 acacggcgcg aattgctaaa gttggcttct tcgccggtat cctgttacct gcatttattt
     1561 tgatcgcatt agcggctatt tatctgcact ccggtgcccc cgttgctatc gaaatggatg
     1621 cgaagacctt cttccctgac ttctctaaag tgggcaccct ggttgtattt gttgccttca
     1681 ttttgagtta tatgggcgtt gaagcttccg caacccacgt caatgaaatg agcaatccag
     1741 ggcgcgacta tccactggct atgttactgc tgatggtggc ggcaatctgc ttaagctctg
     1801 ttggcggttt gtctattgcg atggtcattc cgggtaatga aatcaacctc tccgcaggtg
     1861 taatgcaaac ctttaccgtt ctgatgtccc atgtggcacc ggaaattgag tggacggttc
     1921 gcgtgatctc cgcactgctg ttgctgggtg ttctggcgga aatcgcctcc tggattgttg
     1981 gtccttctcg cgggatgtat gtcacagcgc agaaaaacct gctgccagcg gcattcgcta
     2041 aaatgaacaa aaatggcgta ccggtaacgc tggtcatttc gcagctggtg attacttcta
     2101 tcgcgttgat catcctcacc aataccggtg gcggtaacaa catgtccttc ctgatcgcac
     2161 tggcgctgac ggtggtgatt tatctgtgtg cttatttcat gctgtttatt ggctacattg
     2221 tgttggttct taaacatcct gacttaaaac gcacatttaa tatccctggt ggtaaagggg
     2281 tgaaactggt cgtggctgtt gtcggtctgc tgacttcaat tatggcgttt attgtttcct
     2341 tcctgccgcc ggataacatc cagggtgact ctaccgatat gtatgttgaa ttactggtcg
     2401 ttagtttcct ggtggtactt gccctgccct ttattctcta tgctgttcat gatcgtaaag
     2461 gcaaagcgaa tactggcgtc actctggagc caatcaacag taagaacgca ccaaaaggtc
     2521 acttcttcct gcacccgcgt gcacgttcac cacactatat tgtgatgaat gacaagaaac
     2581 actaaccatg tctgaatggt tataagataa gggagcgata attcatcgct ccctttttcg
     2641 tgcttgctgt cgtattgact taaccgggat aagtacgaga atgagcgcac atctgtttac
     2701 cggaaaccag cacatatgga tatctgctcc cgaaacgaga aattagcgat tagaagacca
     2761 gcgatactgg ttgcactggc acttttactg tgtagttgta aaagcacgcc tccagagtcc
     2821 atggtgacac caccagcggg ttcaaagcca ccagccacga cgcaacaatc gtcacaaccg
     2881 atgcgtggca tctggctggc cacggtgtct cgcctcgact ggccaccggt ttcctcggtt
     2941 aacattagta acaccaccag ccgggcccgt gtacaacaac aggcgatgat cgacaaactg
     3001 gatcatctgc aacgtctcgg cataaacacg gtctttttcc aggtcaagcc agacggtacc
     3061 gccctgtggc catcgaaaat tttgccgtgg tccgatctta tgaccggtaa gattggtgaa
     3121 aatccgggtt acgatccgct gcaattcatg ctcgacgaag cccacaagcg tgggatgaaa
     3181 gtacacgcat ggtttaaccc ctatcgcgta tcggttaata cgaagcccgg tactatcagg
     3241 gaactgaata gcactctgtc tcaacaacca gcgagcgtct atgtgcaaca ccgcgactgg
     3301 atcagaacgt ccggcgatcg ctttgtcctc gacccgggca tccctgaagt tcaggactgg
     3361 atcacatcaa tagtcgcgga agtggtttcc cgctatccgg tagatggcgt gcagtttgac
     3421 gactatttct atactgagtc accagcttca cggctaaatg ataacgaaac gtaccgcaaa
     3481 tacggaggcg catttgcgtc aaaagcagac tggcggcgca acaatactca gcagttaatt
     3541 gcgaaggtat cgcacaccat taaaagcatt aagccggaag tcgaatttgg cgttagcccg
     3601 gcaggcgtgt ggcgtaaccg atcacacgat ccgctcggtt ccgatacccg aggcgcggca
     3661 gcctatgacg aatcctacgc tgacacccgt cgatgggtgg aacaaggatt gctggattac
     3721 attgctcccc aaatttactg gccgttctca cggagtgccg cacgttatga cgttttggca
     3781 aaatggtggg cagatgtcgt taaaccgacc aggacccgcc tgtatatcgg tatcgccttc
     3841 tataaagtgg gtgaaccttc aaagatagag ccagactgga tgattaacgg cggcgtaccg
     3901 gaactgaaaa aacagctcga tcttaacgat gccgtgccag aaattagcgg cacaatcttg
     3961 ttccgtgagg actatctgaa taaaccgcag actcaacaag cggttagcta tctgcaaagt
     4021 cgttggggca gttaaactcc cattcatact ttgtgacaag ccggtggttt acccaccggc
     4081 ttgctaccct cttgaaatat cccactagta atcatgctta cttaagtcaa attaactacg
     4141 caattaacca cacttagtta aacagtcata aatcccctaa taaaaatgta tgaacatgaa
     4201 aaaacatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     4261 aacagtagcg tagattgaaa ctgtcctaag tcgcttattt cttttcagta tatcttcata
     4321 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     4381 ctgctttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     4441 tgagtggacc gggcttgtcg aacaagcaga tccgctcatt cgtgctaaag ccgcggaaat
     4501 tgccgttgcg catgcccatt atctgagtat tgagttttac cgaattgtcc gtatcgaccc
     4561 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     4621 acgctggatt attaacgtgc tttctgccca ggtggacgat gtcgaaaggc taatacaaat
     4681 ccagcatacc gttgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     4741 ggggtttcgg gtgctgaaaa aaatcctcta tccagtcatc ttctcttcgg attattccgc
     4801 cgcagaaaaa cttcaggtct accatttctc tattaacagt attgatatcg ctatggaagt
     4861 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4921 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4981 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagtagttt
     5041 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     5101 cggcatagcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     5161 tcaaaccatg cgtaacacca ggaatttgaa taacagaagt ttacgggtga aatttttatt
     5221 acagataaga aataccgtat cgcaaattat taccttgttg cgtgaattgt ttgaaga
//
LOCUS       DARNVM010000244         5198 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.244, whole genome shotgun sequence.
ACCESSION   DARNVM010000244 DARNVM010000000
VERSION     DARNVM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.244"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..152
                     /locus_tag="SI621_004506"
     CDS             <1..152
                     /locus_tag="SI621_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067133.1"
                     /translation="PVYDDQGRLVHTNTCMKGTTQDNCETAGAIAGGAAAVAGLTMGI
                     IALSK"
     gene            583..1608
                     /locus_tag="SI621_004507"
     CDS             583..1608
                     /locus_tag="SI621_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4067134.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKEANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1676..2857
                     /locus_tag="SI621_004508"
     CDS             1676..2857
                     /locus_tag="SI621_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEI4067135.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFIVTVVIAIAISVGLFRFNKTYQIKTGQLRRTWP
                     VAVMLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            2867..3970
                     /locus_tag="SI621_004509"
     CDS             2867..3970
                     /locus_tag="SI621_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEI4067136.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            3978..4736
                     /locus_tag="SI621_004510"
     CDS             3978..4736
                     /locus_tag="SI621_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEI4067137.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLALHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(4967..5082)
                     /gene="rrf"
                     /locus_tag="SI621_004511"
     rRNA            complement(4967..5082)
                     /gene="rrf"
                     /locus_tag="SI621_004511"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5122..5197)
                     /locus_tag="SI621_004512"
     tRNA            complement(5122..5197)
                     /locus_tag="SI621_004512"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5162..5164),aa:Thr,seq:ggt)
BASE COUNT         1190 a         1230 c         1344 g         1434 t
ORIGIN      
        1 ttccggttta tgacgatcag ggtcgactgg ttcacactaa tacctgtatg aaaggcacga
       61 ctcaggataa ctgtgaaacg gctggggcta ttgctggcgg ggcagctgct gttgctggcc
      121 tgacgatggg gatcatcgct ctgtcgaaat aactgcacaa ggcgcggagt accgcgctct
      181 gtttaacgat acagtatctc tccataagat aaaatattgc tatattctgc tgggtaattc
      241 cctgctaatt tagcatgtta tatcgcgtta tttcgctgct ttcgaaataa cgcatcaatt
      301 taattttcac acttcctacc agaaatatac tcacgctaac aatcattatt ctgcttagta
      361 aaaaatgcgt aaatattttg cgccaaaatg tggcgcatgt ttcattttcg caccattgcg
      421 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
      481 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      541 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      601 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      661 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      721 gggttctctt atgccgatgc tgacggtaag ttttcaggta ttgatgtaga tgtttgtcgt
      781 ggtgttgcgg cagctgtatt tggtgacgac acgaaagtga aatatacccc gctcactgca
      841 aaagaacgct tcacggcttt acagtcaggg gaggtggatt tgctctcccg taatacgacc
      901 tggacttcat ctcgcgatgc cgggatggga atggcattta ctggcgtcac ttattacgac
      961 ggcattggct tcctgactca cgataaagcg ggattaaaaa gcgcgaaaga actggatggc
     1021 gctaccgtct gtattcaggc ggggactgat accgaactca acgttgccga ttacttcaag
     1081 gcaaacaata tgaagtacac accggtgact ttcgatcgct ctgacgaatc agcgaaggca
     1141 ctggaatctg gtcgctgcga tacgctggcc tcggatcaat cacaactgta tgccctgcgc
     1201 atcaaattaa gcaacccagc tgaatggatt gtcttaccgg aagttatctc aaaagaaccg
     1261 cttggtccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg ctggacgctt
     1321 ttcgccatgc tgaatgctga agagatgggc atcaattccc agaacgtcga tgaaaaagag
     1381 gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga ttacggcaag
     1441 gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg taactactcg
     1501 gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg tgggcaaaat
     1561 aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt cggtgtgagg
     1621 taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg tttctatgtc
     1681 tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg ttcgtgcttg
     1741 gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt tatttcacaa
     1801 cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc tggatcgcgg
     1861 cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca cctacggacg
     1921 cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta ttgtgttcgc
     1981 ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc tgctacgaaa
     2041 gctttccaca atttatattg agatcttccg taatattccg ccgctgctgc aaatcttctt
     2101 ctggtacttt gccgtgttgc gcaatttgcc cggaccacgc caggctgtga gcgcgtttga
     2161 tctggccttt ttgagcaatc gtgggcttta tattccgtca ccgcagctgg gagacggatt
     2221 tattgcgttt atcgtgactg ttgttattgc tatagccatt tctgttggac tattccgttt
     2281 taataaaacg taccagataa agaccggaca attgcgccga acctggccgg tcgccgttat
     2341 gttgatcata ggtttgcctt tactggcgca atggcttttt ggcgcagcac tacactggga
     2401 tgttccagcc ctacgaggct ttaacttccg tggcgggatg gttttaattc ctgaactggc
     2461 agccttaacg ctggcgcttt cggtttatac ctctgcattt atcgccgaga ttatccgcgc
     2521 tgggatccag gcagtgcctt atggtcaaca tgaagcggct cggtcactgg gattacccaa
     2581 tccggttacg ctacgccagg tcattattcc ccaggcactg cgagtgatta ttccgccgtt
     2641 aaccagccag tatctcaaca tcgtcaaaaa ctcctctctt gccgccgcga ttggctatcc
     2701 ggatatggtt tcgctgtttg ctggcaccgt gctgaaccag acggggcaag ccatcgagac
     2761 gatagccatg accatgtcgg tctatctgat tatcagcctg actatctcgc tgctgatgaa
     2821 tatctataac cgccgcatcg cgatcgttga acgctaagga gtcatgatga caaaagtatt
     2881 gctgtctcat cccccgcgcc cggcgagcca taactcaagc cgcgcgatgg tgtgggtgcg
     2941 aaaaaatctg ttctccagct ggagcaatag cctgctgact attggctgca tatggttgat
     3001 gtgggaactg attccaccgt tgctgaactg ggcatttttg caggctaact gggttggctc
     3061 aacgcgtacc gactgcacaa aagccggtgc ctgttgggtc ttcatccacg aacgatttgg
     3121 tcagtttatg tatgggcttt acccacacga ccaacgctgg cgaattaacc tcgcattact
     3181 gattgggctt gtgtcgatcg caccaatgtt ctggaaaata ctcccgcatc gcggtcgcta
     3241 tattgcggta tgggcggtga tttacccact gattgtctgg tggctgatgt atggcgggtt
     3301 tcttggtctt gagcgggttg aaacccggca atggggcggg ctgacgctaa ctttaattat
     3361 tgcatcagtt gggattgcgg gggcgctgcc gtgggggatc ttactggcgt taggtcgccg
     3421 ctcccatatg ccgattgtgc gtatcttatc ggtcattttt atcgagttct ggcgcggtgt
     3481 accgctgatt accgttctgt ttatgtcttc ggtcatgctg ccgttgttta tggcagaagg
     3541 caccagtatc gacaaattga tccgcgcgct ggttggcgtg atcctgtttc agtcagcata
     3601 tgttgcggaa gtcgtgcgag gcggattaca ggcgctgcct aaagggcaat atgaagcggc
     3661 agagtcgctg gcgttgggtt actggaaaac ccaggggctg gttattctgc cacaggcgtt
     3721 gaagctggtg attcctgggc tggtaaatac catcatcgca ctcttcaaag ataccagcct
     3781 ggtgatcatc atcgggttgt tcgatctttt cagtagcgtt cagcaggcaa ccgttgatcc
     3841 cgcctggttg ggtatgtcga cggaagggta tgttttcgcc gcactgatct actggatctt
     3901 ctgtttcagc atgtcacgtt atagccaaca tctggaaaaa cgttttaaca ccgggcgtac
     3961 accgcattga ggacactatg agccaaattt tactgcaacc tgctaacgcg atgattacgc
     4021 tggaaaacgt caataaatgg tatgggcaat tccatgtttt gaaaaatata aatttaaccg
     4081 tgcaaccggg agaacggatc gttctgtgtg gcccttctgg ttccggtaaa tcgacaacca
     4141 ttcgttgtat taatcatctg gaagaacatc aacagggacg gatcgtggtg gatggcatcg
     4201 aacttaatga agatatccgc aatattgagc gcgtcaggca ggaagtggga atggtctttc
     4261 agcatttcaa tctcttccct catctgaccg ttctacagaa ctgtaccctg gcaccgattt
     4321 gggtacgcaa gatgcctaag aaagaggctg aagatctggc gttgcattac cttgagcggg
     4381 tgagaattgc cgaacatgcc cagaagtttc ccggacagat ttctggtggt cagcagcaac
     4441 gcgttgccat agcacgttca ctttgtatga aaccaaagat catgctattt gatgaaccta
     4501 cgtcagcgct cgatccggag atggtgaaag aagtgctgga tacgatgatt gggctggcgc
     4561 agtcgggtat gacaatgttg tgtgtaacac atgagatggg gtttgcacga accgtcgctg
     4621 acagggtgat ttttatggat cgtggggaaa tagtggagca agctgcacct gatgaatttt
     4681 ttgcgcatcc taaatcagag cgtacgaggg catttttatc gcaggtaatc cattaattga
     4741 atgttagttc gaaaagcaaa aaggccatcc tttcggatgg cctttcgctt gatttgatgt
     4801 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
     4861 aaatccgctc ccggcggatt tgtcctactc gggagggtgt tcaccgacaa acagcagata
     4921 aaacaaaagg cccagtcttc cgactgagcc ttttgtttta tttgatgtct ggcagttccc
     4981 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
     5041 cggcatgggg tcaggtggga ccaccgcgct actgccgcca gacaaattct tttctaatct
     5101 gccgaacttt aacctaaaaa gtggtgctga tacccagagt cgaactgggg acctcaccct
     5161 taccaagggt gcgctctacc aactgagcca tatcagca
//
LOCUS       DARNVM010000245         5093 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.245, whole genome shotgun sequence.
ACCESSION   DARNVM010000245 DARNVM010000000
VERSION     DARNVM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.245"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..359)
                     /locus_tag="SI621_004513"
     CDS             complement(<1..359)
                     /locus_tag="SI621_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HEI4067138.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDTGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRP"
     gene            complement(371..877)
                     /locus_tag="SI621_004514"
     CDS             complement(371..877)
                     /locus_tag="SI621_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067139.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANG
                     NAEISWKI"
     gene            complement(881..2101)
                     /locus_tag="SI621_004515"
     CDS             complement(881..2101)
                     /locus_tag="SI621_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HEI4067140.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGELSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2116..2850)
                     /locus_tag="SI621_004516"
     CDS             complement(2116..2850)
                     /locus_tag="SI621_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEI4067141.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGHYDGSNDSITEIIE
                     ALERYSKIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNRATEAVVTGGRAEHFAKEIAASGDIAKSRVDLI
                     ARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGELPNCRCYKEIVFPTSHSYPA"
     gene            complement(2741..4207)
                     /locus_tag="SI621_004517"
     CDS             complement(2741..4207)
                     /locus_tag="SI621_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HEI4067142.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMIDPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPLSNQPTQDSAGGRRHRQWSLRWFK"
     gene            complement(4207..>5093)
                     /locus_tag="SI621_004518"
     CDS             complement(4207..>5093)
                     /locus_tag="SI621_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HEI4067143.1"
                     /translation="IPVFTFHWRDDPRKDEEWYRRECEKIDNPVVVAQELDLNYSASA
                     EGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAFSTRHGFLLENVREW
                     SGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAINELRNAARRPSILA
                     TPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLRKLFQNTWRAVVEGM
                     AYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPDGTRSPNLADSVMIN
                     YAPMNSALNIWELLGRQA"
BASE COUNT         1076 a         1450 c         1291 g         1276 t
ORIGIN      
        1 atcgggcggc cgacctgctg agcagcctgc aattctatga ccgtccagcc aagttccatc
       61 ccccacaggt tcagcgggtt accggatttg ccggtatcca cgttcacgcc agcaatagcg
      121 gttgagtctt tgcctaccca gtttttgcca ttcggatttg caccagtacc cgcagcggcg
      181 aagctggtat tcgtccagct ggaaatgtca tctgcgatag agacatcttc acgcaactga
      241 atatcgcggg tccaggtgta ccccaccagt ggcaggttca gcgtctggtc gagtcgctcc
      301 agctccccga tgagaaaggc accagagctg tcaacggttg cctgatcaaa agtaatcatt
      361 cgtctgttcc ttaaatcttc caggaaattt ctgcattgcc gttagcatca ccggcacctg
      421 tgaattcagc gttggtcagc accacatttt tgccactgac tgacgtggac atgaatccac
      481 ccagcggcac tttgatggat tcatcagtgg agacgacaac gtataccggg tcgccttttt
      541 tgatagtgct ggcatcaaaa tcagaaccga gattaacggt cacgtagcca cgcttcatgg
      601 cgtcgcccgg gaagttcttg cctgttccca cctggcgaac catgtccggc tgcgacgtgg
      661 tcggataagg gcgcacgtag atccccttca ccttgtctgc ggtatcacca tctgccagcg
      721 gcacgaaaaa accgtcatca tcgtatttac cagccagccc ataggcagcg aaggcgttat
      781 cggatttaag gaccaccggt tcgacggtta agtcctgcgg gcgagagaca gccccggcaa
      841 tgccaacagg catccggtac agaaatacat tattcatttt ttacccttta cggtttgccc
      901 agaattcagc gttttgtttg ttcagggaag cgatactggt catgcccatg tttaggcgct
      961 gtgcatcgcc ggtggtggcg cgggtgtttc gccctttggc aatctcagac acggcattaa
     1021 acgccatgtc gaccgattgt ttcggtaatt tgcggatatc cgcatcgccg actatctggc
     1081 gaaccagcgt tttgtcagca gaagccagaa cctcgcgttt gaacgcggtc ggtttcatct
     1141 tacggctcag atcgataccc ggaacgataa cttcggcacg ccaggctgag tcaccagtaa
     1201 tcgtggtttc ctcttcatcg tcctcgccgt caccggtcgg attatcgtca ggcttattgt
     1261 cgttatcgcc cgtcgcattt ccttccagct tagccagcag ggctttcagt aatgttttga
     1321 ggtcatcatc actgtcgccg gttggacctc cgcccatctc tggtgctttg tccggtagcg
     1381 gttgctgcgg ggacaggttg atgttgagat taacgccctg cggcaaatcc ccctcatctc
     1441 ctgtaaccga tgcgggagcc gactccacca gttcgttcat ggtgtcagcg tcacccgttt
     1501 tgatggccgt gcgcatgcgg gtccaccagc ttttcttttg atttgccatt gtgtctctgt
     1561 ctccaattgc acaacgattt ccggctctgc ctttagggac aagagccaca tggtttccgg
     1621 taatatcgac ctgctcggct ttacctggct cggtctgctc atactccgcg tcatagccgc
     1681 acgacacttc gcgcaggcca tcttcgataa gctgaatggc gctttcgtct ttgacgataa
     1741 ggtcagccag catcaaatca gacagctcac ccgtcccgcg ccggacattc tggaggtgcc
     1801 cgacagcaag ctctttccag ttctcgggat ttaccagccg cacattcccg ttttcatctt
     1861 caggatgcag aatcgtgatg ctcatccctt cgaatgaggc aagcgtggcc ggatggaata
     1921 cctgctcagg agaacgcgtg acgactattt caccgaactt atcgggtttc agttttggca
     1981 ggtcatcagc accatagagc tgcttacctg ttcgtcctat cggcacgtct ttgcacagca
     2041 acgagccgtc agccagctga tagcgggttt cccccagccg ggtattgaaa aaatatttca
     2101 tgtgttacct gcgattcagg cgggataaga atgggaggtg ggaaaaacga tttctttata
     2161 acagcgacaa ttcgggagct cgccagcgtg accggtcatg ccatcaagcg ttggaggttt
     2221 gccccattcg acaaatttac cttccatttc ccgatgagaa tgcctgacgt caccatcttc
     2281 ggctgtacgc cagatataac cattcgaacc aattgacagc gcacgcgcct gatccagcgc
     2341 gccggttgca cgtccaagtt cagtacgggc aatcaggtca actctggact ttgctatatc
     2401 acccgatgcg gctatttctt tagcaaaatg ttctgctctc ccgccggtca caacagcttc
     2461 tgtcgcccga ttctggatgt cgtacaccct gtcagccgcc tcgaggggga gcgatttgat
     2521 gtacttgacc tgttcggcga tgatggattg catcacctgg cccacaggag cgctttccac
     2581 aagattgcgg agctcgcgac tgatgttctt gctgtgttgc cgccaaactt tctcgttctg
     2641 ccgggttagg tccgcagtaa agttttccgc gacctttgtc gcccaggggg tgatgatttt
     2701 actgtagcgt tccagcgcct caataatttc cgtgatactg tcatttgaac catcgtagtg
     2761 accattgacg atgtctccga ccgcccgcgc tatcctgcgt aggctggttc gatagcggat
     2821 ttccgcctga cggttcctgc ggttcgtcat caggttcgcc gatgccgggc ggcgcttcat
     2881 cttcggcatt ctcgatgtcc tcgtcggtaa tggatgcccc gatgccggtt acgtcagaat
     2941 tttcgcgcaa atcagtcata gcggctttca gtgtcatcag accatcaccc agcgccgtac
     3001 tgattgcgtt ggtggtgttt aacgccaccg ttgagcgatc gacatcagac atttgccaga
     3061 gcgggttaaa ctcaaacgtg aaatcatccg ggagcggctt gccaagttcc gaacgatgca
     3121 tgatgtccag tatccgacgc accggaagac gtaaacgcct ctcctgcaac gaactgatgc
     3181 ggtcgtaata gttggcaagg tctgcatcac cggtagaaaa tcctttcggg gactgtccga
     3241 acaaccgcac cagtgggata ccaacagcgc cactaatctg ttctgcaaac tgtgatagga
     3301 tgtcatccag accactgaag ctgtactgat gcgtttcaaa cttatcccgc gagtccatga
     3361 gcgtcatgcc ttcattgctc tggaactgtc gaatcaggtc gatattcttc agcaacgctt
     3421 catacgcagg accaccaagt gcgataagct cgcgtagctt ctccacgctg taggtacgca
     3481 aatgcgcttt gtagaccagc tgcgccgcgc cgacagtagc gctgtcgaac gcggtaagac
     3541 gatcccagat acgctctaca accgacattc cccattcgtt ctcggtcatc ttctgctgaa
     3601 atggcagcgt gacgccatca aagcgaatca gtcgactgtg atgaatgcgc caggcaggaa
     3661 ttcccgttgc tgtggtcacc acatcgtaaa actcaggttt acccaggtcc ggccccatat
     3721 ctttaatgcg gcgggtcagt accgggtcaa tcatccagcg gtcgagcggg agaatcccct
     3781 taaacttgcc cttaccgatg gtttcgggtc gcagcggggt cattggtgcc tgcccctcaa
     3841 tcatgatgaa acccaccgcg ccgccgtaga ggcgcgacca tttcagcacg tcattcagcg
     3901 catcccagat ttgcaactca tccagttgtg attcgagaat gccgcgatct tttgcatcaa
     3961 tttccgaagt gatgcgaatg cctttgcggg tcatatcatc cgggatagca tcgaccgctt
     4021 cgccgataat ccaggacgaa cgataggacc attccaccag catgcggtta cgactggtga
     4081 aattagcccg gtaggtggat gctgagtgct ggttaggtgt ctgcatccct acgcgggcaa
     4141 taaaattctc ataaccatcg gctgtggcct gcacagttcg ccgcagggct tgtttgtttc
     4201 gtgccatcag gcctgtctcc ctagcaactc ccagatgttc agggctgaat tcattggggc
     4261 atagttgatc atcaccgagt cggcaaggtt tggcgaccgg gttccatcag gctgtttatc
     4321 aataacgatt tttcccacac cattaatgga ataggtcggc tgcgaaagct cgatgatgag
     4381 tttatctttg agtgccatgc tactgctgat tgagatgatt tcgtccgggt tgtaagccat
     4441 accttcaacc acggcgcgcc aggtattctg aaaaagttta cgtaaccgcc accagctctg
     4501 ggctttggcg ttagcgaaga agtccttgtt cagacgtgcg gcttgcccgt tgtccccgcg
     4561 aacagcttca tcatccggat caaataccgc gccactacct cgaaacggtg tggcgagtat
     4621 tgacggtcga cgcgcagcgt tacgcagttc gttgatagcg cgtgcatcgc cgcgaacgcc
     4681 agcgcccagt ccgtcctcgt caaagcgaaa ctcttcgagg ttatcctgtt cgcaaaagcc
     4741 gaaaaccttc tcgacggact gataaatgtc gctgcccaca ccggaccatt cccgcacatt
     4801 ttccaggagg aagccatgac gggtggaaaa ggcatttttg tccctgcctt cgtcggcgac
     4861 atccatcgcg ccaagtcgtt tgcctgttgg ctggataccc agtttgatat gcgcatcaac
     4921 ggcagcctgt acccattcgg atggaatcag gacgccttcc gctgatgcgc tgtagttcag
     4981 atcaagttcc tgtgccacca ccaccggatt atcgattttc tcgcattccc tgcgatacca
     5041 ctcttcatcc ttgcgaggat catcccgcca gtggaatgtg aataccggta tct
//
LOCUS       DARNVM010000246         4958 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.246, whole genome shotgun sequence.
ACCESSION   DARNVM010000246 DARNVM010000000
VERSION     DARNVM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.246"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            262..528
                     /locus_tag="SI621_004519"
     CDS             262..528
                     /locus_tag="SI621_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEI4067144.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            525..1124
                     /locus_tag="SI621_004520"
     CDS             525..1124
                     /locus_tag="SI621_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HEI4067145.1"
                     /translation="MMVPVLQKLPFSGLPFSGICGYSFSAAAKSAAGRGNPSMLSATY
                     DAPCVFFCVAINATERQIMVWCVVSRANGTPCILPASAHYTAESMVAQAGQPPGWPVS
                     DNAGISTPVWAIAIERGNSGDSVIYAVIGGCLMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1117..1404
                     /locus_tag="SI621_004521"
     CDS             1117..1404
                     /locus_tag="SI621_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HEI4067146.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1397..1852
                     /locus_tag="SI621_004522"
     CDS             1397..1852
                     /locus_tag="SI621_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067147.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            1988..2308
                     /locus_tag="SI621_004523"
     CDS             1988..2308
                     /locus_tag="SI621_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HEI4067148.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2323..4656
                     /locus_tag="SI621_004524"
     CDS             2323..4656
                     /locus_tag="SI621_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEI4067149.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGREATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRCLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLENHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT         1079 a         1446 c         1379 g         1054 t
ORIGIN      
        1 tgctgtttat tactttttat ttttattaat tcactgaaat aaataagaaa aaacaatttg
       61 tatttcacta taattttttc cagctccttc cagagcgttt taaagctatc tggcgctgat
      121 gtacaaaaaa tacacagcca ttgtaaggct gccggaacaa atcccccctg ttgcgtctgc
      181 cgaaaatatt cacaaaataa agcgctaccc gaagccggac ggacttatcc ggtgctgtat
      241 ggacattaac gaggtagccc gatgcaagct gttttttctt ccccgtctcc cgcccctgtg
      301 acgccactga tgccgctgcc ggacatcacg caggagcgtt ttttacgtct gccggaagtg
      361 atgcacctgt gcggcctgtc acgctcgacc atctacgaac tcatccgtaa gggggaattt
      421 ccgccgcagg tgagtcttgg cggtaaaaat gtggcctggc tgcactctga agtcaccgca
      481 tggatggccg ggcgcattgc cggacgcaaa cgggggtacg acgcatgatg gtgcctgttc
      541 tgcaaaaact tcctttttct ggcttgcctt tttccggcat ttgcggatat agtttttccg
      601 ctgccgcaaa atcggcagcc gggcgtggaa acccgagtat gttatcggcg acatatgacg
      661 cgccatgcgt ctttttttgt gtcgcaatca acgccacaga gcgccagatt atggtgtggt
      721 gtgtggtcag tcgagcaaat ggcactccgt gcattctgcc cgcgtccgct cattataccg
      781 cagagtcaat ggtagctcag gcggggcagc ctccgggctg gccggtttcc gataacgccg
      841 gtatttccac ccccgtctgg gctatcgcca tcgagcgtgg aaactccggc gatagcgtca
      901 tttacgctgt tatcggaggt tgccttatgg ctacgaccct taccccctca caccctgaat
      961 ttgtctttgt gtttgcggct gtccgtcgcg cagaccgtca tccccgtatc tgtatgcttc
     1021 gcaccgtcgc cggtgatgaa cgcagcgccc gccgttccct tgtccgtgac tatgtgctct
     1081 cccttgctgc ccgtctgccg gtagtggagg tgtcccgtgc gtaataaaaa agcccctcag
     1141 accgtctcag cgcgtcatga cgcccgtgaa cacctcagca ttgaggctta ccataagctc
     1201 aaccgcgcca gcgccgtatc ccggtttgtt gggggtgatt tgattcaccg tgaactctcc
     1261 ggcctgcatc agctctacat tccgcatatt ttcagctacc tgaatgaaga tattgatttt
     1321 gtgctgaatg agctgaaagc caaaggcctg tgccgcgatt ttctcgccca gcagaaagac
     1381 cggggagaca ggacgcatgt ttgattttcc ccagcccggt gagatttacc gttctgccgg
     1441 ttttccggat gtggccgtgg tcggcattct ggaagacggt attccgtggg aaatgccgta
     1501 ccgctgcccg gacattgtct ggaacccgta ccgccgtaaa ttcagtatcc ttgtgcgtat
     1561 cctcgccgac gggcgcacca cagacatccc gctggggcgt tttctgcggg aatttacctg
     1621 tgaccgtcct gacctgttta aacgcagccc cgtaaaccgg catgcggtac tgaaagaaat
     1681 ggccggagac ccggaattac agaaatggcg ggagaaatat ctggatattt acccgcagga
     1741 cactgttccg gtcagccggg cggcaccggt ggcgcgggaa tggcgggaaa ttccccgcac
     1801 ggagcctgac ccggaaatca caccggataa cagttaccgc aattatctgt aattaaaaaa
     1861 cgacacccga aaaattaaat gtgcgtattc gcgcagggat acgcacgtct tcaggagacg
     1921 cagatatgcc ttatcagtta atgcaaccgg cacggaatgc agtcatctgt cacagggagg
     1981 aaagaaaatg aaaacaccct taccgcccgt cttacgcgca gccctttacc gtcgtgctgt
     2041 cgcctgtgcc tggctgaccg tgtgcgaacg tcagcaccgc tacccgcatc tcacccttga
     2101 atcactggag gcggccatcg ccgctgagct ggaaggcttt tatctgcgcc agcacggtga
     2161 ggaaaaaggg cgtcagatag cctgtgccct gctggaagat ttaatggaat ccggccccct
     2221 gaaggccgcg ccgtcgctgt cctttctcgg gctggttgtg atggatgaac tctgtgcccg
     2281 tcacataaaa gcgccggtac tgcactgaag gagaacaaca ccatgaaaat gaacgtaacc
     2341 gccaccgtca gccatgcgct cggccactgg ccgcgtattc tcccggcgct ggggattcag
     2401 gtgctgaaga accgtcatca gccctgtccg gtctgtggcg ggagtgaccg cttccgtttt
     2461 gatgacaggg aggggcgcgg cacctggtac tgcaatcagt gtggtgccgg tgacggcctg
     2521 aaactggttg aaaaggtgtt tggtgtttcc ccgtccgacg cggccgcaaa ggtggctgcc
     2581 gtgaccggca gcctgccccc ggctgacccg gcagtgacag ctgccgccgg tgctgaaaca
     2641 gacgccgccc ggaagaacgc cgccgcactg gcacaaaccc tgatggcgaa aacccgtccc
     2701 ggaaccggta acgcctacct gacccgcaag ggctttcccg gccgggaatg ccggatgctg
     2761 accggcacac acagagccgg tggtgtgagc tggcgcgccg gtgaccttgt ggtgccactg
     2821 tatgacgaca gcggcgaact ggttaacctt cagttaatca gtgctgacgg ccgtaagcgc
     2881 accctgaaag gcggacaggt caggggcacc tgtcacaccc ttgaaggaca gaatcaggcc
     2941 ggaaaacgtc tgtggatagc ggagggatac gcgaccgcac ttaccgtgca tcacctgacc
     3001 ggtgaaacgg tgatggtggc gctttcttcc gtgaacctcc tttctctggc cagccttgcc
     3061 cggcagaagc atccggcctg tcagattgtc cttgccgcag accgtgacct cagcggtgac
     3121 ggccagaaaa aagccgccgc agccgcagat gcgtgtgagg gtgttgttgc cctgccgccg
     3181 gtcttcggtg actggaatga tgccttcacg cagtacggca gggaggccac ccgtaaggcc
     3241 atttatgatg ccatccggcc accggctgaa agcccgttcg acaccatgag cgaagcggag
     3301 ttttccgcca tgagtaccag cgaaaaggcc atgcgtatct atgagcatta cggcgaggcg
     3361 ctcgcggtcg atgccaacgg ccagcttctg tcccgctatg aaaatggtgt ctggaaggtg
     3421 ctgccgccac aggactttgc ccgggatgtg gccgggctgt ttcagcggct gcgcgcgccg
     3481 ttctcctccg ggaaggtggc ctccgtggtg gacaccctga agctgattat tccgcagcag
     3541 gaagccccct cccgctgcct gattggcttt cgtaacggcg tgctcgacac gcagaacggc
     3601 acgttccacc cgcacagtcc gtcacactgg atgcgtaccc tgtgcgatgt ggatttcacc
     3661 ccgccggtgg acggtgaaac gctggaaaac cacgcccccg cgttctggcg ctggcttgac
     3721 cgtgccgccg gtggccgtgc ggaaaaacgc gacgtgattc tggccgcact gtttatggtg
     3781 ctggcaaacc gctacgactg gcagctcttt ctggaggtga ccggtcccgg cggcagcggc
     3841 aaaagtatca tggccgaaat agccaccctg ctggccgggg aggataacgc cacgtcggcc
     3901 accattgaga cgctggaatc cccgcgtgaa cgtgccgcgt taactggctt ctcactgata
     3961 cgcctgccgg accaggaaaa atggagcggc gacggtgccg gactcaaggc catcaccggc
     4021 ggcgatgcgg tgtccgtgga cccgaaatac cgggatgcgt actccacgca tatcccggcg
     4081 gtgattctgg ccgtgaacaa taacccgatg cgcttcaccg accgcagcgg cggcgtgtca
     4141 cgccggcggg tgattattca cttcccggaa cagatagccc cgcaggagcg cgacccgcag
     4201 cttaaggaca aaatcacccg cgagctggcg gtcatcgtgc gtcacctgat gcagaagttc
     4261 agcgacccga tgctcgcccg gtcactgctt cagtcccagc agaactcaga cgaggcgctg
     4321 aacatcaaac gggatgccga cccgacgttt gattttatcg gctatctgga aaccctgccg
     4381 cagaccagcg gcatgtatat ggggaacgcc agtatcatcc cgcgtaatta ccgtaaatac
     4441 ctctatcacg cctatctggc ctacatggag gcaaacggct accggaatgt actcagtctg
     4501 aaaatgttcg ggctggggct accggtgatg ctgaaggaat acggactgaa ttacgagaag
     4561 cgccatacca aacaggggat acagaccaac ctgacactga aagaggaaag ctacggcgac
     4621 tggctgccaa aatgtgacga ccctgcgaca acctgaccat catgactcat ctgaccggca
     4681 tctgccggtc tttttttatc ccgacatccc ccgaaggtga acaatccact gttcaccctt
     4741 caccgtatat tcacccgtta tcacactgaa attaaaagag aaaaacgaaa ggtgaacagt
     4801 gtgaacaatc aaatcaaaaa aaactttttt ctccctgcgt gattttcagt gcaggggact
     4861 aatcgccggt atgagtcaca ccggcagaat gccggaggtg aagaatcgaa tgttcaccct
     4921 tcacccatta ttcaccacct atcactatga aataaaag
//
LOCUS       DARNVM010000247         4958 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.247, whole genome shotgun sequence.
ACCESSION   DARNVM010000247 DARNVM010000000
VERSION     DARNVM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.247"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..479
                     /locus_tag="SI621_004525"
     CDS             <1..479
                     /locus_tag="SI621_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067150.1"
                     /translation="SCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIKLEWSGD
                     KRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWS
                     LYPNGDGCVTISSPEIKNKYPAAICLALGFFISIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(722..1417)
                     /locus_tag="SI621_004526"
     CDS             complement(722..1417)
                     /locus_tag="SI621_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEI4067151.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTELNQQ
                     QRCDAFIQFASDVMLTRCELTSEAQAAEAAIRLCKELGDQSVVISDDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1410..2837)
                     /locus_tag="SI621_004527"
     CDS             complement(1410..2837)
                     /locus_tag="SI621_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEI4067152.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGSETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVCIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2848..3567)
                     /locus_tag="SI621_004528"
     CDS             complement(2848..3567)
                     /locus_tag="SI621_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEI4067153.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSC"
     gene            complement(4094..4948)
                     /gene="rclR"
                     /locus_tag="SI621_004529"
     CDS             complement(4094..4948)
                     /gene="rclR"
                     /locus_tag="SI621_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HEI4067154.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVENESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1306 a         1309 c         1060 g         1283 t
ORIGIN      
        1 ttagctgtga tagcaataat aactttgcct gtcagaccac aatgactgaa gatgttattc
       61 agcgaattac tacatttttt cagaccagcc cagatgtcaa gaacagagaa ataaagctgg
      121 aatggtcagg agataagaga gatttaccaa ctgctgaagc ggaaatatct cgcgtgcagg
      181 cgtctattat caaatggtat gcgtcagaat atcataatgg caggcaagtt ctcgatgaga
      241 tacaaacacc ttcagcaatt aacagtgagc tttatacaaa aatgatatac ctgaccagga
      301 actggtcatt gtatccgaac ggtgatggct gtgtaactat aagctcacca gaaataaaaa
      361 ataaataccc tgctgccatt tgcctggctc tgggattttt tataagcatt gtaatttctg
      421 taatgttttg ccttgtcaaa aaaatggtag atgaatacca acaaaactct gggcagtaat
      481 aaacagcatt ctgacagaga gtgcctgtat aaaactatag tgacaatgac cgggaaggga
      541 gcccgtcttc aggaatggat tcctgtcttt ttcactgtaa atatcctggc attactaatg
      601 ccaggatatt tacagaagag cagcataacc atgacggaac ggggttaagt caggttgtcc
      661 ttaacgctat tcacaacctc gtatgccgcc ttcaggttta tgtccagact tcatatctct
      721 ctcagcaatc ctcaataatc agatacaccg ctttcaccgg gccatgaacc ccgacgactt
      781 tgataagctc aatatccgcc gttgaactag ggccgctaat gatgttaatg caggacggca
      841 ttcgttcacc ggcctgcgct ttctgatgca atttttctgc gagttgcgct acacgcggca
      901 ggattgtgct tttacgcagg ataaaaagag aagattccgg gagcaggctc aatgaacgcc
      961 cgcgctcggc ggcggaaaaa agaaccacgc ctcccgattc ggttaaacca tattcagcat
     1021 acacgacacc cactttagcc tgctctgcct gcgagatatt ctcggcacct ttcgccggat
     1081 cccaaacaac ggcattgcat tcctgctgca aacgttcgct aatccctaat tcctccagcc
     1141 tcgtgtcatc gctaatcacg accgactgat ctcccagctc tttacacagt cgtattgcag
     1201 cttctgccgc ctgcgcctcg ctggtcagtt cgcagcgcgt caacataaca tcgctggcaa
     1261 actggataaa cgcgtcacag cgctgctgtt ggttaagttc ggtaagccgc tcgttagcat
     1321 agttgttaag cggcgcatct tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct
     1381 gagcaacgtt attcaaaaat tcgctccgat tatccattct ttttctcctg cgcctgatgt
     1441 ttcttaaacc aactacggaa actctctccg tcagcttcag gaagatcgcg tgcttccatc
     1501 cagtcgctaa tcgcgccaaa tttgagtggt gttttgccgc cattgataaa ccagcttgcc
     1561 gcatgagcgc cggccatcat cccgactttc cacaatcctg gatgactatt ggcataagcg
     1621 aacattttta tcgcccgttg ctctgctttt gcggtgatcc ctttttcagc catcacccga
     1681 cgatgacgca aaatcagttt tgacagcgga atacacaccg gacacacgct gtcacaagcg
     1741 gtgcataaag agcaggcgta gggtaaatct ttaaaatctt tatagccgcc aagtagcgga
     1801 gaaatcaccg caccaattgg ccctggataa atagagccat atccatgacc gccaatatgg
     1861 cgatatgccg gacaagtatt catacaagcc ccgcagcgaa tacagcgcag cacatcccga
     1921 aattcagagg ccagcacctc agaacgcccg ttatcgacaa taaccagatg aaactcttca
     1981 ggaccatcaa cgtgccccgc ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa
     2041 cgtgcaccaa cggcactgcg cgccagcatg gtgatcaata catctacctc ggcaaacgtg
     2101 ggggcaatac gctccattcc catcactgca atatgcgttt taggcagcgt ggtacacatt
     2161 cgcgcattac cttcattggt caccaggcat accgaacctg tctctgccac cgcgaaatta
     2221 cagccggtaa tacctatttc agcactgagg aaatcttcgc ggattttttg ccggatgaat
     2281 aaggtcattg cttcaggcgt ttccgacccc tcatagccca gacgttcgtg tagcacccga
     2341 cggatctgat ggcgatcttt atgaattgcc gggaccacaa catgagaggg cggatcttga
     2401 tccagctgga gaatgtattc acccagatcg gtttcaatca cctgaatgcc agcatcctgc
     2461 aacacatgat tgacaccaat ctcttcggtc accatcgatt tagatttcac caccttccgg
     2521 gcatttttgc gttgggcaac ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca
     2581 aaatagacgt gaccgccgtt ttgcgtcact ttttctgaga gctggtacag ataagcgtcg
     2641 agattactca gaacatgatc acgtatctgg gcggcccgat cgcgccactc ctcccagtgc
     2701 cccaattcat cgaccatttt ttgccgattt gccccgatac gctgctgcgc gtttgccacc
     2761 gctttgcgca tgatcggatc ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta
     2821 ctggttttga tcgacatctt tatatcctca gcagctcatc aacacttcag caatatgcat
     2881 cactttgact ttctgccctt cccgttgtaa tcgcccactg atgttcagca ggcaactcac
     2941 gtcagcacct attaaatact cagggcggac ttccatcagg tgcgcaacct tttctttcac
     3001 catctcgccg gatatttcgg ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt
     3061 atcctgttca gcaaaggtca acagctccag tccacgcaca tttttcagca gcgtaagtgg
     3121 ctcgtccttc acccccagct tacgggccag gctacaagat gggtgataca ccgctctccc
     3181 ttgcaaactg gcacctacat cgactacccc taatgtatta acaataaaag aggtgagatc
     3241 ctgcatacgc gcggcaacct tttcggcacg tgatgcccat tcaggctcat ccgccagata
     3301 cgttgggtaa ctttttacgg cataggtaca ggagccagcc ggtgaaataa tgggatcgtc
     3361 gttatcctcc agtgcggcga tcagattttt catccctgga atcgcttctt tgatataacc
     3421 actattgatc gcaggctgac cgcagcatcc ttgtttctcc gggaaattta cgcgacagcc
     3481 gagtttttcc agtagcagca cggagtctcg tgccattctt gatttcaggg cgtcaccaat
     3541 acaggtgaca aagaaattga cattcacaac taatactcca ttacttcatg cccatttatg
     3601 cccgatggtt ccccccaccg ggggatcaat catcaataaa tatggcgttc ttttttaatt
     3661 ggctagcaac ataccacccc tcatttgtat gacaaatgat aatctttcat ccaaacttca
     3721 aatgcaataa ctaattttat ttctttcggc atcgctcaca ttttataggg aaatcgacga
     3781 tcatttttgg cataagaaat ccatttaaaa aggctacatc ccttatacct attcagtgct
     3841 acttattcag gggttattga gatatacact gatggtgtac aaacaaaatc accaacaata
     3901 attacgtatc aagtaataat cggcatttta tttaattgaa gcaatttagt ttgcattttt
     3961 atttaaaaac tgagctagag tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg
     4021 aaatagaaat agttgaaagg ctcacaactc ccggaatgcg gattatcttt gccctgattt
     4081 ctggcggttt tattcagggt gcaagctgtc tgaccctttc ccgatattct cccggagtac
     4141 aaccaaactc gcggacaaac gccttgtgaa aagatgattc actggcatag cctactgact
     4201 cagcgatcac cacaacaggg agcatttccc gggaaaacat ctgggccgct atttgtagac
     4261 gcaactttgt taatacagct agcggcgtgg ttccggaaac atcacggaaa agctgggcaa
     4321 aacttgcccg ggacatgtgg gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg
     4381 gcatttccag catttgctgt attaccgcac caagacgtgg atgcagaagc aaactgagaa
     4441 tgtttttctc tgtattaacc tgtgcaatcc actcacgcac cgcgagggta aagaatgtag
     4501 cgcagatctg gctacacaga gcatccactc ccggcattgc cgatctggat tcctgttgta
     4561 aaaacgggat cgcctccctc agccagttat attccacgct atggttcact ggtgctacaa
     4621 acagcgtttc cggcaaagac gttaaaaaat aacgcgccga atgttgcagc cgaagagtgc
     4681 cacagacaat acaggtcgat tcattttcga catgactcag gcgatgagct gaattttgtg
     4741 gtagcaggac cacatttccc gggcgtaatg taaaaatctc tcccgtcggc atttccagct
     4801 tcgccgctcc ttgcgttaac gcatgccaac gaataaccga caattccccg gcaccatgtg
     4861 gaagttgcca gtcacttcct aacacgcaat tcttatcgat cgttccttgt ggagcgttaa
     4921 gcatcaacaa acggctaaga gcatccatat cagactcc
//
LOCUS       DARNVM010000248         4956 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.248, whole genome shotgun sequence.
ACCESSION   DARNVM010000248 DARNVM010000000
VERSION     DARNVM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.248"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..460
                     /gene="espX1"
                     /locus_tag="SI621_004530"
     CDS             <1..460
                     /gene="espX1"
                     /locus_tag="SI621_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HEI4067155.1"
                     /translation="YGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSF
                     TNTKIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLIQTL
                     VRDNVKLNNDVYKELNKFMHDYDKTSPEMRKYLQSINECMLLMKNIVHQD"
     gene            762..1295
                     /locus_tag="SI621_004531"
     CDS             762..1295
                     /locus_tag="SI621_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEI4067156.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLSAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            1345..2028
                     /locus_tag="SI621_004532"
     CDS             1345..2028
                     /locus_tag="SI621_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEI4067157.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAEKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2041..4485
                     /locus_tag="SI621_004533"
     CDS             2041..4485
                     /locus_tag="SI621_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEI4067158.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDFYVNHQWRGKKDITIPESPAKPCLPKVLLTTLGVKTDNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFTAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLIIPHRENHLVLDIANSETELQGNRQIIAPYRGAVSYVQFTTDQRKPWY
                     IQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCT
                     ITFQHVIDENKTYICQ"
     gene            4498..>4956
                     /locus_tag="SI621_004534"
     CDS             4498..>4956
                     /locus_tag="SI621_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HEI4067159.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCAVTNVGEQRGTYILKPLSMKGNL
                     TTEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKLSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSS"
BASE COUNT         1643 a          970 c          942 g         1401 t
ORIGIN      
        1 atatgggtat tattttggtt gtgtattatc caatatatca tgttttgaaa gcgacttatc
       61 aaacaccatc ttttcaaatg gagaaataaa taacctcttc attaaaaaaa gcaacatatt
      121 tggaacatcg ttcactaata ccaagataaa aaatttgcgc tgtgaagata ttatgcctgg
      181 tcgatggacc actcaacttg tcaataagca cttaggttac agatatacag gagtattcaa
      241 aaccctggca agtattgatg acaaaccgtc ccgtttcgaa attttgatac cattgataca
      301 aactttagtt cgcgacaatg taaagctaaa taatgatgtg tataaagaac ttaataagtt
      361 tatgcacgat tacgacaaaa catccccaga aatgagaaaa taccttcaga gcatcaacga
      421 gtgtatgctc cttatgaaaa acatagttca ccaagattaa aatactcaaa gaaagaagta
      481 atttcagaat gaaattgtct gtgatgatta ttaatacact gcagagaaat aaaaaacaat
      541 ctgaagcatg ctctgaaaca tcctactctg tcactctaat gatggcacct tgctcttctt
      601 ataaagaagg gaacaattaa gaatattaaa ttttcaaagt ttcatcaact tcacatcaag
      661 aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata atcgggtttg
      721 aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag tatattattc
      781 ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat gtcgatcaag
      841 gaacgctaac cattagagga gaagttgttg atacaacgtg ttactttgtt aatggtgatt
      901 cgacaacaga tatcgttgtc gaagatgttg taaccagtgc aatgaatcaa cttatcaata
      961 atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt cagtgtgacg
     1021 gcaatacagc cccaagtatc gaagtgctag catcagagtt tgatgctaat aactttacgc
     1081 tgaataaagg aactgctgag aacattggct ttgccatttt cctcgatgat ggaactttga
     1141 ccagaatgag accggggtta aaaacaccgc tgaaagataa tggctctggt caatattttc
     1201 ttaatttaag tgcacaatat gcccgcacaa gtggcaatcc tgttacaaaa ggctccgtag
     1261 agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta gctctgctac
     1321 cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat gtcatttttt
     1381 attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta tccggaaaaa
     1441 aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt gattcaggca
     1501 tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtccc atttattctc
     1561 acaccgccag taagtcgtgt agcaggtggt tctggccaac aacttaaaat aagaaaaatg
     1621 ccaaacaatt tgcctcacaa taaagaaagt ttattttatc taaatgttct ggatatccct
     1681 ccaaacaacc cacaaaatgc agaaaaaaac aaaattaaac ttgcattgca aaaccgtata
     1741 aagttacttt ggcgtccaag cggaatcgct ccggtagata aaaaaagcct ttctcaatta
     1801 aatatcaaaa agaaaaataa cgccatctca ataaacaacg aaacagccaa ctggataacg
     1861 gttacaacta tcaaagcaca aaacgtgaaa gttaataatg aaagtatctt actgcctcca
     1921 ttttcgaata acgacattac attaaaaaat aaccacgcat cagaatatga actaacggta
     1981 gttgatgatt acgggaataa catccatagt aaaatcgccg cgagataaca ggaaaaagtc
     2041 atgttaagga cgacacgatg ggtcgctgct attatttttt tgtattcttt tccgggttat
     2101 gctgaagaaa cttttgatac gcacttcatg attggcggca tgagaggaga aaaagtttcc
     2161 gaatatcgtt tcgataataa acaaccgctt ccaggaaact acgaacttga tttttatgta
     2221 aatcaccagt ggcgtgggaa aaaagatatc acgatcccag agtcaccagc caaaccatgt
     2281 ctgcctaagg tgctgctaac cacactgggg gtaaagaccg ataacctcaa tacagaagat
     2341 aattgtattt tactggatga agcggttcat ggtggacaat atcagtggga tatcagtgaa
     2401 caccgactaa atttaacagt accacaggcg tatatcaatg aacttgaaag aggctacgtt
     2461 cccccagaaa gttgggatcg cggcattgat gctttttata cctcctataa tttaagtcag
     2521 tatcgctctt atgacagcaa taataacagt aatacagcca gttatggccg gtttaacagc
     2581 ggattaaatt tatttagttg gcagttacac tctgatgcca gttatagcaa acctgatgat
     2641 atgaaaggca aatggcaaag taatactctt tacctggaac gtggctggtc gcaaatatta
     2701 agcacagttc agataggaga aaattacaca tcttcactca tattcgactc tctgcgattt
     2761 agcggaatac gcttatttcg cgatatgcaa atgctacctg attctatgca aagctttaca
     2821 cctttagtac aaggtgttgc gcaaagtaac gccttgataa cggtttcgca aaatggctat
     2881 accatttacc aaaaagaggt tcccccagga ccatttacta tcgcagattt gcaactttcg
     2941 ggcagcggct ctgatctcga tgtcagtatt aaggaagcag atggtagtgt tcgttccttt
     3001 ttggtgccct actcttccgt gcccaatatg ctgcaacctg gtgtttcaaa tttcgatttt
     3061 accgccggac gcagtcagat atatggtgtc aagaatcagg aggactttct cgaggcaaat
     3121 tatatatatg gcctcaacaa tcttttgaca ctttatggcg gtacgatttt atccgataac
     3181 tataatgcta taactctggg aaatggttgg aatacaccgc tgggagcaat ctcttttgat
     3241 gctacccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
     3301 gtcgcctata ataaatatct attgcaaacg gctactcact ttagcgtggc cgcctggcgt
     3361 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaatga taaaataaat
     3421 catcagagtg actatgacga tttttatgat attggaagaa aaaatagcct ttctgccaat
     3481 atcatgcaac ctttatccaa taatctggga aatgtatcat taagtgcgct ttggcggaat
     3541 tactgggggc gaagtggaaa tgctaaagat taccaattca gttattccaa tagctggcaa
     3601 cgcatcagtt atactttctc tgccagccaa tcctatgatg aaaacgacaa agaagaggag
     3661 cgttttaatc tgtttatctc cattcctttc tactgggggg atgatattgc caaaacacgt
     3721 caccaaatta acttatcgaa ttcgacctca ttttccaaag atggctattc ctccaacaat
     3781 actggaatta ctggcatagc cggtgaacat gatcagttaa attatggtat atatgttaat
     3841 cagcaacaac aaaataatga tacctcgctt ggtacgaatt taagctggag aactcccatc
     3901 gccacaatag atggcagcta tagccattct aaaaacgcct ggcaaagtgg tggaagtatt
     3961 agtagtggat tagttgtctg gcccggtggt attaatataa ctaaccagtt atccgataca
     4021 tttgcaattc tggatgcgcc tgggttagaa ggcgcgcata ttaatggaca aaaatacaac
     4081 cgaacaaaca gcaaaggcca ggttgtttac gacctgatta tacctcatcg tgaaaaccat
     4141 ctggtgcttg atatagcaaa cagtgaaaca gaattgcagg gcaatcgtca aattattgcg
     4201 ccttaccgtg gagcagtttc ttatgtgcag tttacaactg accaacgtaa gccctggtat
     4261 atacaggcac tgcgtcccga tggttcgcca ttaacctttg gctatgacgt actggatctc
     4321 caggaaaaca atattggagt cgttggccag ggtagtcgcc tttttattcg cgtagatgaa
     4381 attcccactg gcataaaagt tgctctcaat gatgaacaga acttattctg tactattact
     4441 tttcaacacg ttatcgatga aaacaaaaca tatatatgcc agtaaaagga gtttactatg
     4501 aagtggcttt tattaatcac cttgagtcta tattctttta ttgtgcaaag tgctccctgt
     4561 gcagtgacca atgtcggtga acaacgtgga acatatatac tcaagccact gagtatgaaa
     4621 ggtaatctaa ccaccgaaaa aattgaaaac accgggaata tcattgagtg tgcagacttg
     4681 caagagacga ccaacgccat taaagttcat tttttaacac agaacgcttt aaggtttaaa
     4741 gtcggaagtc gtcattacat tattaatttc ccctttgaat ctgacggtaa aaaaatagag
     4801 cttccaggga agttatctta taatttagaa gaaatgctat ctgcgataaa tttcaccatt
     4861 ccatattcta tcgcatctgt atcagatagt tctaacaccg cagacatttt tccaggaact
     4921 ccttttcctt tgttgttcga aattgaagtg agttca
//
LOCUS       DARNVM010000249         4814 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.249, whole genome shotgun sequence.
ACCESSION   DARNVM010000249 DARNVM010000000
VERSION     DARNVM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.249"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..593)
                     /locus_tag="SI621_004535"
     CDS             complement(<1..593)
                     /locus_tag="SI621_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEI4067160.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="SI621_004536"
     tRNA            complement(708..783)
                     /locus_tag="SI621_004536"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="SI621_004537"
     tRNA            complement(790..864)
                     /locus_tag="SI621_004537"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="SI621_004538"
     tRNA            complement(981..1065)
                     /locus_tag="SI621_004538"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="SI621_004539"
     tRNA            complement(1074..1149)
                     /locus_tag="SI621_004539"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="SI621_004540"
                     /gene_synonym="panK"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="SI621_004540"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEI4067161.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3455)
                     /gene="birA"
                     /locus_tag="SI621_004541"
     CDS             complement(2493..3455)
                     /gene="birA"
                     /locus_tag="SI621_004541"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEI4067162.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAE"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="SI621_004542"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="SI621_004542"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEI4067163.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLKDYRGTVIINRIKGIEIHDELDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="SI621_004543"
     rRNA            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="SI621_004543"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1206 a         1225 c         1107 g         1276 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgggtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaatgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgcgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacagtgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgctgtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcacgactga tggcttcctt caccctaatc aggtactgaa
     1921 agaacgtggt ttgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcacctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaacggtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt tcctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagcg attaatactg ccatgacgtt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgcgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa gaggtcaggc tacgcaaata
     2461 attttcaggg gagctaatgc tcccctatct tattattccg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagca ataaagcgcc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccagcttttc ccagcgcgaa agataaggtg ccaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatg agcatggccg ccaacgtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccttgctcca gacgccagaa catcgacaaa
     3061 tataagtttg cgccaaaagg cgaaaaccat ttccgccccc ggcgaccacg gccagcctgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atctgttcag cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaatagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatggtgt gccagttgca ccacatcttc gctttttgca ttgtcttcat
     3601 taatgagaac taacgcctgt tgacggtgca ccgcagcccc acccatttgc atccctttta
     3661 gctggcactg atcgataagc caacctgctg ccagttttac tgaaccatcc gcctgggggt
     3721 aatttggtgc tgttggaaat tgtgacagta atgctttagc cgtttcggca gatacaacag
     3781 ggtttttgaa gaaactaccg gcattgccat tcacttttgg gtcaggaagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac tgttgtagga tccagacgag
     3901 ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcggtcctgg tattcatgtt taaaaatact gtcgcgatag ccaaaacggc
     4021 actctttggc agttaagcgc acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 cacaaccagg aattaacgcc agattttcca gacctggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaagttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tctttcagaa
     4321 aaagtacatt acttccttca cccagaataa gaacgggttg cccttctgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tccgtggggg gatggcttgt agtaatgacg acagggagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttctgcttaa tttgatgcct
     4621 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     4681 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct acggccgcca ggcaaattct
     4741 gtttcatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc
     4801 aatccgccaa aaca
//
LOCUS       DARNVM010000250         4800 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.250, whole genome shotgun sequence.
ACCESSION   DARNVM010000250 DARNVM010000000
VERSION     DARNVM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.250"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            8..1342
                     /gene="garP"
                     /locus_tag="SI621_004544"
     CDS             8..1342
                     /gene="garP"
                     /locus_tag="SI621_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HEI4067164.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1358..2128
                     /gene="garL"
                     /locus_tag="SI621_004545"
     CDS             1358..2128
                     /gene="garL"
                     /locus_tag="SI621_004545"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HEI4067165.1"
                     /translation="MNNNVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2158..3048
                     /gene="garR"
                     /locus_tag="SI621_004546"
     CDS             2158..3048
                     /gene="garR"
                     /locus_tag="SI621_004546"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEI4067166.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIAEVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3145..4290
                     /gene="garK"
                     /locus_tag="SI621_004547"
     CDS             3145..4290
                     /gene="garK"
                     /locus_tag="SI621_004547"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HEI4067167.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNVG"
     gene            4323..4699
                     /gene="rnpB"
                     /locus_tag="SI621_004548"
     ncRNA           4323..4699
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SI621_004548"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1115 a         1155 c         1388 g         1142 t
ORIGIN      
        1 aaataaaatg attctggaca ccgttgacgt aaaaaagaaa ggcgtgcata cccgctattt
       61 aatattactg attattttta ttgttaccgc cgttaactac gccgatcgtg caacgctgtc
      121 tattgctggt accgaagtgg caaaagagtt gcagttaagt gcggtttcga tgggttacat
      181 cttctccgct tttggctggg cctacctgct gatgcaaatc cccggcggct ggttgcttga
      241 taagtttggc tcgaaaaaag tttacaccta cagcctcttt ttctggtcgc tgttcacttt
      301 cctgcaaggc tttgttgata tgttcccgct ggcctgggca gggatctcca tgttctttat
      361 gcgctttatg ctcggcttct cggaagcgcc atcattcccg gcgaacgccc gtattgtcgc
      421 cgcctggttt ccgacaaaag aacgtggtac tgcctccgcc atctttaact cggcgcaata
      481 tttctcgctg gcgctctttt cgccgctgct tggctggctg actttcgcct ggggctggga
      541 gcacgtcttc accgttatgg gggtgattgg ttttgtgctg acggcgctgt ggatcaagtt
      601 gattcataac ccgacagatc acccacgtat gtctgcggaa gagctgaagt ttatctcaga
      661 aaatggcgcg gtggtcgata tggaccacaa aaagccgggc agtgcggcag caagcggtcc
      721 caaactgcat tacatcaagc aattgctctc taaccgcatg atgctgggcg tatttttcgg
      781 acaatatttt atcaacacca tcacctggtt cttcctcacc tggttcccaa tttatctggt
      841 gcaggaaaaa ggcatgtcga ttctgaaagt gggtctggtc gcctcgattc cggcactgtg
      901 tggctttgcg ggcggcgtgc tgggaggtgt cttctcggat tatctgatca aacgcggttt
      961 gtccctgacc ctggcacgta agctaccgat tgtgctggga atgttgctgg cttccaccat
     1021 catcttatgt aactacacca acaacaccac gctggtggtc atgctgatgg cgctggcttt
     1081 ctttggcaaa ggatttggtg cgctgggctg gccagtgatt tctgacaccg cgccgaaaga
     1141 gattgttggt ctctgcggcg gcgtctttaa cgtctttggc aatgttgcct ccattgtcac
     1201 tccactggtg attggctatc tggtaagtga actgcactcc ttcaatgcag cactggtttt
     1261 cgtgggatgt tcagcgctga tggcgatggt ctgctacctc ttcgtagttg gcgacattaa
     1321 acgtatggaa ttgcagaaat aagcaaaggt ataagcgatg aataacaatg ttttcccgaa
     1381 taaattcaaa gccgcactgg ctgcgaaaca ggtacaaatt ggttgctggt cagcactctc
     1441 taacccgatt agcactgaag ttcttggttt ggctgggttt gactggctgg tgctggatgg
     1501 cgaacatgcg ccaaacgata tctccacgtt tattccgcag ttaatggccc tgaaaggcag
     1561 cgccagcgcg ccagtagtgc gagtgccgac caacgagccg gtaattatta agcgtcttct
     1621 ggatatcggt ttttataact tcctgattcc ttttgtagaa acaaaagagg aagcagagca
     1681 ggcggtggca tcaacccgtt acccaccgga aggcattcgc ggcgtctccg tttctcaccg
     1741 cgccaatatg tttggcaccg tggcggatta tttcgctcag tcgaacaaga acatcaccat
     1801 tctggtccag atagaaagtc agcagggcgt agataacgtc gatgccattg ccgctaccga
     1861 aggcgtagac ggtatcttcg tcggccccag cgatctggct gcggcattag gccatctcgg
     1921 caatgcctcg cacccggatg tacaaaaagc aatccagcat atttttaacc gtgccagtgc
     1981 gcacggcaaa cccagcggta tcctcgcacc ggtcgaagcc gatgcgcgtc gttatctgga
     2041 atggggcgcg acgtttgtgg ctgtcggcag cgatctcggc gtcttccgct ctgccactca
     2101 gaaactggct gataccttta aaaaataacc accaccgcaa caagagagat gattgatatg
     2161 actatgaaag ttggttttat tggcctgggg attatgggta aaccaatgag taaaaacctt
     2221 ctgaaagcag gttactcgct ggtggttgct gaccgtaacc cagaagctat tgctgaagtg
     2281 attgctgcag gtgcagaaac agcttctacg gctaaagcga tcgctgaaca gtgcgacgtc
     2341 atcataacca tgctgccaaa ctcccctcat gtgaaagagg tggcgctggg tgagaatggc
     2401 attattgaag gcgcgaagcc aggtacggta ttgatcgata tgagttctat cgcaccgctg
     2461 gcaagccgtg aaatcagcga agcgctgaaa gcgaaaggca ttgatatgct ggatgctccg
     2521 gtgagcggcg gtgaaccgaa agctatcgac ggtacgctgt cagtgatggt gggcggcgac
     2581 aaggctattt tcgacaaata ctatgatttg atgaaagcaa tggcgggttc cgtggtgcat
     2641 accggggaaa ttggtgcagg taacgttacc aaactggcaa atcaggtcat tgtggcgctg
     2701 aatattgccg cgatgtcaga agcgttaacg ctggcaacca aagcgggcgt taacccggat
     2761 ctggtgtatc aggcaattcg cggtgggctg gcgggcagta ccgtgctgga tgccaaagcg
     2821 ccgatggtga tggaccgcaa cttcaagccg ggcttccgta ttgatctgca tattaaggat
     2881 ctggcaaatg cgctggatac ttcccacggc gtcggcgcgc aactgccgct cacagctgcg
     2941 gttatggaga tgatgcaggc actgcgagca gatggtttag gaacggcaga tcatagcgcc
     3001 ctggcgtgct actacgaaaa actggcgaaa gtcgaagtta ctcgttaatg acgtcgtgcc
     3061 cggatggcgt attgcaatcc gggcctggaa tccaggccga ataagagaaa cgccctccgg
     3121 cgtcatgtgg taacaggcat aggtatgaaa atcgtaatcg ccccagactc ttataaagaa
     3181 agtttatctg ccagcgaggt tgcgcaggcg atagaaaaag gatttcggga aatttttcct
     3241 gatgcacagt acgtttctgt tcctgttgcc gacggtggcg aaggaacggt ggaagcgatg
     3301 attgcagcca cccagggttc cgaacgtcac gcctgggtta cagggccgct gggcgagaaa
     3361 gtgaatgcca gttgggggat ctccggcgat ggcaaaaccg cgtttattga aatggcggcg
     3421 gccagtgggc tggagctggt acctgcggaa aaacgtgatc cactcgtgac cacttcacgc
     3481 ggcacaggcg agttaattct gcaggcgctg gagagcggcg cgacaaacat tattatcggc
     3541 attggcggta gcgctacaaa tgatggcggt gcaggcatgg tacaggcgct gggggcgaaa
     3601 ttatgcgacg ccaacggcaa tgaaattggt tttggcggcg gtagtcttaa tactctgaat
     3661 gatattgata tttccggcct cgatccgcgc ttaaaagatt gcgtcattcg cgtcgcttgt
     3721 gatgtcacca atccgctggt gggcgataac ggcgcatcgc gcatctttgg cccacaaaag
     3781 ggagccagtg aagcgatgat tgttgagctg gacaataacc tctctcacta tgccgatgtc
     3841 attaaaaaag cgctgcatgt tgatgtgaaa gatgtccccg gcgcaggagc tgcgggaggt
     3901 atgggcgcgg cgctgatggc gtttcttggt gcggaactga aaagtggtat tgaaatcgtc
     3961 acgacggcgc tgaatctgga ggaacatatt cacgattgta cgctggtgat caccggtgaa
     4021 gggcgtattg acagccagag tattcacggc aaggttccga ttggcgtcgc aaacgtggcg
     4081 aaaaagtacc ataaaccggt gattggcatt gcgggtagcc tgaccaatga tgttggcgtt
     4141 gtacatcagc atggcattga tgcggtcttc agcgtattga ccagcattgg tacgttggac
     4201 gaagcattcc gcggggctta tgacaatatt taccgtgctt cacgtaatat cgccgcgaca
     4261 ctggctattg gaatgcgcaa cgtggggtga caagggcgcg caaaccctct atactgcgcg
     4321 ccgaagctga ccagacagtc gccgcttcgt cgtcgtcctc ttcgggggag acgggcggag
     4381 gggaggaaag tccgggctcc atagggcagg gtgccaggta acgcctgggg gggaaaccca
     4441 cgaccagtgc aacagagagc aaaccgccga tggcccgcgc aagcgggatc aggtaagggt
     4501 gaaagggtgc ggtaagagcg caccgcgcgg ctggtaacag tccgtggcac ggtaaactcc
     4561 acccggagca aggccaaata ggggttcata aggtacggcc cgtactgaac ccgggtaggc
     4621 tgcttgagcc agtgagcgat tgctggccta gatgaatgac tgtccacgac agaacccggc
     4681 ttatcggtca gtttcacctg atttacgtaa aaacccgctt cggcgggttt ttgcttttgg
     4741 aggggcagaa agatgaatga ctgtccacga cactataccc aaaagaaagc ggcttatcgg
//
LOCUS       DARNVM010000251         4786 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.251, whole genome shotgun sequence.
ACCESSION   DARNVM010000251 DARNVM010000000
VERSION     DARNVM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.251"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            32..763
                     /locus_tag="SI621_004549"
     CDS             32..763
                     /locus_tag="SI621_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067168.1"
                     /translation="MERLLDAYKRILQEVDAQSFNLNEDKYSGVFLPVPFEEYWHSPV
                     KIMLVGRETAGWNTLNGKNTISRVLGLIPDVTIGQVVEEAVDRYRKHLPAQNDGTTNH
                     KSRSRFTQYHFRLARELNIPPQAIVYANLLAWDYDGLPPLNRPQNEVQEVILASLKLL
                     AVQIKHLEPDFTIFASGARRTDYIIKQVLTELGGYETSSVIPGKLWEFKTGHTICFRI
                     AHPRAMRGHQKYRDEVIARIKQLCA"
     gene            complement(1016..1102)
                     /locus_tag="SI621_004550"
     tRNA            complement(1016..1102)
                     /locus_tag="SI621_004550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1066..1068),aa:Leu,seq:taa)
     gene            complement(1115..1188)
                     /locus_tag="SI621_004551"
     tRNA            complement(1115..1188)
                     /locus_tag="SI621_004551"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1154..1156),aa:Cys,seq:gca)
     gene            complement(1243..1318)
                     /locus_tag="SI621_004552"
     tRNA            complement(1243..1318)
                     /locus_tag="SI621_004552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1283..1285),aa:Gly,seq:gcc)
     gene            complement(1470..2018)
                     /gene="pgsA"
                     /locus_tag="SI621_004553"
     CDS             complement(1470..2018)
                     /gene="pgsA"
                     /locus_tag="SI621_004553"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEI4067169.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2075..3907)
                     /gene="uvrC"
                     /locus_tag="SI621_004554"
     CDS             complement(2075..3907)
                     /gene="uvrC"
                     /locus_tag="SI621_004554"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEI4067170.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEDIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3904..4560)
                     /gene="uvrY"
                     /locus_tag="SI621_004555"
     CDS             complement(3904..4560)
                     /gene="uvrY"
                     /locus_tag="SI621_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HEI4067171.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1206 a         1260 c         1151 g         1169 t
ORIGIN      
        1 aggtattttt attacggggc aggattgaag aatggaaaga ctattagacg catacaaacg
       61 gatattacag gaagttgacg cccagtcatt taacctgaat gaggataaat actcaggcgt
      121 ctttttaccc gtgccctttg aagaatactg gcactcaccg gtaaaaatca tgctggttgg
      181 gcgtgaaacc gctggctgga atacgctgaa tggtaagaat acgatatcac gggttctggg
      241 acttatacct gacgtcacca tcgggcaggt ggttgaagag gctgtagatc gttacaggaa
      301 acatcttccg gcacaaaatg atggcaccac gaaccacaaa tcacgtagcc gcttcacgca
      361 gtatcacttc cggctggccc gggagcttaa tattcctcct caggctatcg tgtatgccaa
      421 tctactggcg tgggattacg acggactgcc ccctctcaat cgccctcaaa atgaagtgca
      481 ggaagtcata ttagcctcgc tgaaactgct ggcggtacaa attaaacacc ttgagcctga
      541 tttcaccatc tttgcatccg gagcccggag aacagactac atcataaaac aggtgcttac
      601 tgagctaggc ggctatgaga cttcttcagt cattccgggg aagctatggg agttcaaaac
      661 aggtcataca atctgtttca ggatagccca tccaagggct atgcgcggac accagaaata
      721 cagagatgag gtgattgcac gaattaagca actttgtgct tagggtggcc agagcattat
      781 agcttgctca gctggccccc cttcccggaa gagtaccgga aaacaggcaa agcggcctgg
      841 ttctctatgc tggaggcgac acgttcgatt cggcattgcc gggcttgaga ttgcagctca
      901 ttgccccccg tgagcacagg atatgcacag ggcgtgaatt tcaggcacaa aaaaaccacc
      961 cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta
     1021 cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt
     1081 ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg
     1141 gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg
     1201 cctttcagcc agacatccgc ttgacgccga tgccttttaa actggagcgg gaaacgagac
     1261 tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcat
     1321 tcatcaagca atcagttaat cacttgattt tattatcgtc tggcaatcag tgccgccgtt
     1381 cgatgcgttg cattctactt acctggcgcg atgagtcaac gatatttttc accacttttg
     1441 atcgtttgct gaaaattgcg ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg
     1501 ctcaaatatt gcaacattga ccacagagtc agtaccgcag ccacaaagaa aagtgcaata
     1561 ccggcgtact caacccaaat gttcggacgc cacagcagcc aggccagcgc caccatctgg
     1621 gcggtggttt tcactttccc aatccaggag acagccacgc tactgcgttt acccaactcc
     1681 gccatccatt cgcgtagcgc agaaataata atttcacggg cgatcatcgt tgctgccggt
     1741 aaggtcaccc accagctgtg ataatgctcg gttaccagca ccatggcgat agccacgaga
     1801 actttatctg ctacagggtc aaggaaagca ccaaaccggg tactctggtt ccagcggcgt
     1861 gccagaaaac catcgaacca gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca
     1921 aacggcgacc aggtgacagg cagataaaag accaatacaa agaatgggat aaggatgaca
     1981 cggaacagtg taagcaacgt agggatatta aattgcataa tgacgggtaa ctatctgttg
     2041 tcagtaagat tacccctatg ttgctacaga gacatcaatg tttcaacgac cagaagatct
     2101 tttctgccag accttgcgaa atacccggca cttttgcaat atcctcaacg ctggcgttac
     2161 gtaaaccttg caaaccgccc atatatttca acaacatttg ccgacgtttt ggcccgacgc
     2221 cttcaatggt ttccagggaa ctggtatttt tgaccttcgc ccgtttctta cggtgcccgc
     2281 caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat aacatgcagc gcgggagaat
     2341 ctggcggcaa actgaatccc tcaccttccg gctcaaagaa cagcgtttcc aaccctgcct
     2401 tcctatctgc acctttggca acgccaagaa gcagcggatg atttttatcc catgagacat
     2461 ccagttcggc gaagacattt ttcgcctgcg caagctggcc tttgccgccg tcaataagga
     2521 tcacatccgg gatcttactg tcgtcgatgg ctttaccata acgccgacgc agcacctgat
     2581 tcatcgccgc ataatcatcg cccggcgtga tgccagtaat gttatagcgc cgatactccg
     2641 cacgcagcgg gccgttagca tcaaacacca cacaggaagc gacggtttgt tcacccatgg
     2701 tatggctgat gtcaaagcac tccatccgct tcacttccgg caatttcaac acgctggcaa
     2761 gcgcggtcag tcgctggtga acggtagatt gctgcgaaag tttgctggtt aaggccgtcg
     2821 ccgcattggt gcgcgcgagt ttcagataac gcgcccgatc gccgcgaggt ttggtttgaa
     2881 cattaatctt gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa
     2941 gattaaaatc gagcaggatc tcacccggta aggtgcgcat ctggctgcct tgtaaataga
     3001 actgacctac gaaggtttct accacctcgc tcagttccgt accgccaggc actttcggga
     3061 aatagctgcg gctgccgagc actttgccct gacgaatgaa caatacatgg acacaagcca
     3121 tgcccgcatc gaacgccaca ccaataacgt cgaggtcgtc gccggtattg gacacaaact
     3181 gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgcc gcttcttcaa
     3241 actccagatt ctggctggca gtttccatac ggctaatgag ttgcgtaagc acctgatcat
     3301 ctttgccaga caaaaacagg cgcacatact cgacctgctg agcgtattct tcttcactca
     3361 ccagtccttc aacgcacggt cccagacagc gccctatctg atattgcaga cacggacgcg
     3421 agcgattgcg ataaacacta ttttcgcact ggcgaatggg gaatatcttt tgcagtagcg
     3481 ccagtgtttc acgtacggca tagccattcg ggaacgggcc gaaatattca cctttggcat
     3541 gcttcgcacc acgatgcatc gccagacgcg ggtgggtatc gccactcagg aagataaaag
     3601 gataagattt atcatcgcgt agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt
     3661 tgtgttccag caacagcgct tcggtttctg tatgagtaac cgttacatca atttgctgga
     3721 tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa
     3781 gccgtttttt caggtctttc gctttgccga catagataac cgtaccacca gcatcataca
     3841 tgcgataaac gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat
     3901 cactcactga cttgataatg tctccgcatt acacagacca tggcgaattg ccaggtgagt
     3961 cagctcaaca tcgccatgaa tgtttagttt actgaacata cgatagcggt agctgttcac
     4021 cgttttcgga ctgagattga gctgttctga gatctcattg accttctggc ccttggtgat
     4081 catcagcata atctgcaatt cacgttcaga caaactggca aatgggcttt ctgttttttc
     4141 tggttcgatc tggcttaacg ccatttgttg agcgatgtca gaagcaatgt aacgctgccc
     4201 tgaatagaca gaacggatcg cactcacgac ttcctgcgga gccgcgcctt tgctgaggta
     4261 gccagcagca ccggcctgca tgactttcgc tggtaaaggg ttttctgtat ggacggtaag
     4321 catgatgatt ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc
     4381 aatgcccggc atactcatgt ccattagcac cacgtcaacg gcatttgccc ggcaccactt
     4441 aacggcgtct tcaccgcacg atgcctcacc gacgactttt atacccttta tatcttccag
     4501 aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca acaagtagaa cgttgatcaa
     4561 aggaatatct ccagaaatag ggataacgct actgatagtt agtcattcgt atattagcgg
     4621 tttttattgc aactttgaaa cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg
     4681 taaattcgat tgtccacagt tatggtcacg cccgtcacca acggcttccg ggatcgtttc
     4741 actgtaagcc ctgcgatgaa atgtgacaaa aattgacttt attcag
//
LOCUS       DARNVM010000252         4769 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.252, whole genome shotgun sequence.
ACCESSION   DARNVM010000252 DARNVM010000000
VERSION     DARNVM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.252"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(479..925)
                     /locus_tag="SI621_004556"
     CDS             complement(479..925)
                     /locus_tag="SI621_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HEI4067172.1"
                     /translation="MMINEAHAQATAASGRGDGQYPSGLHDGAEISTAAGGQTKAEVP
                     LTMEAVITRENLMLAYQRVVENKGVAELKPWLKKNWRSVWQVLIDGNYQPRAIRRMDI
                     PKPDGGVRTLGIPTVVDRLIQQAIAQQLSAIVDTASAATQTTATST"
     gene            1291..1518
                     /locus_tag="SI621_004557"
     CDS             1291..1518
                     /locus_tag="SI621_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HEI4067173.1"
                     /translation="MEDFHLLVVLQPPQLKNSASHGATRHARRTYATSADVNGTRNIL
                     AAGHAVLACKEMVQSGLLLKQEPTEMIPAIA"
     gene            complement(1571..2932)
                     /gene="dcuC"
                     /locus_tag="SI621_004558"
     CDS             complement(1571..2932)
                     /gene="dcuC"
                     /locus_tag="SI621_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HEI4067174.1"
                     /translation="MIKVVIALLVIVIVARLILKGYRAEPVLFIAGLTLMICTWFTGW
                     GTVLPKGVSGTGISFLDPFEVMRNLFSTRAADLGLMIMTLMGFAHYMDHIGANEAVVR
                     VVTRPLRTLRSPYILLFFSYLFASLLQLAIPSATGLAVLLMGTMFPIMLGLGLSAASA
                     AGVIATSLGVAYTPTAIDAIRGSEAVNMDVVEYVVYHQGPAALATVLIVGISHFFWQK
                     HCDRKAGTLPHEIGTTTVMESGSTPAYYALLPMLPILMAVGSSEIFVTGINLNIITIV
                     LISMAICMLIEWVRKCDLKAVCDGFTHFLKGMGTAFTGVVGLLVAAGVFAHGIKSIGA
                     IDQLILMAEHVGLPPFAMGIVFALVTLAAAVIMGSGNAPFLAFVELIPQIAASMGVNA
                     ISMILPMQQASHMGRAMSPVSGVVIAVSSGANITPFEVVKRTALPLIVGFVFHSAIIG
                     IFY"
     gene            complement(2929..4185)
                     /gene="pepT"
                     /locus_tag="SI621_004559"
     CDS             complement(2929..4185)
                     /gene="pepT"
                     /locus_tag="SI621_004559"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEI4067175.1"
                     /translation="MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSEL
                     EALGMEDIILRENAIVTATLPANTDETIPVVSFFGHLDTSAEQTADTKAQRLPYNGGD
                     LCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHS
                     EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEI
                     IFTGQSAHPMSAKGKLKNSLLMAHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSART
                     VLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVTCQLSDRYANVLNSLQGKVHY
                     PIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRS
                     LRAASDVVVAVIQETFNEFTTENRES"
BASE COUNT         1268 a         1236 c         1021 g         1244 t
ORIGIN      
        1 tttttccatt atgtttattt gacttctatc aaaaaaaatt atgctaattc gattttgcaa
       61 aatttaattg cacgcaaata atatgcacat attttttagt gtaatgagtg acttttatgg
      121 atagattccc gctgacctct atcaccatac ttccccccgc tttcagtagg acatccgtgt
      181 ctgactttat atcgagattt catgtggaag agataaatgc cggaccttga gaaagaaata
      241 taactcccca gtctcgttga accctcttgt tgatccactt ttcaagtacg taacgatcca
      301 agttccgcct gccgtccaag gcgtttggtg aagcagcacg gtccgtcact gtcagcccac
      361 aacttccgct cccacgggcg cgccaccgcg ctcttcttct cattcacctt ctgtttcagt
      421 ttgctctcca cgaacgccct gatgtccttc agcagatggt cgcccgcttt ccggctgctt
      481 acgtagatgt tgcagtcgtc tgcgtagcgg cagaagctgt gtccacgatg gcgcttaact
      541 gctgcgctat cgcctgctgg ataagcctgt ccaccaccgt cgggatgcct aaggttctca
      601 cgccgccatc cggcttcggg atatccatcc ggcgtattgc ccgcggctgg tagtttccgt
      661 caatcaatac ctgccatacg ctgcgccagt tcttcttcag ccacggtttc aactccgcca
      721 cacctttgtt ttccaccacg cgctgataag ccagcatcag gttttctctc gttatcaccg
      781 cttccatcgt cagcggcact tccgctttcg tttgcccacc cgccgccgtg ctgatttcag
      841 cgccatcatg cagccctgac ggatactgtc cgtctcctct gccgctggcc gcagttgcct
      901 gcgcgtgtgc ttcattaatc atcatcgggt tcggatgtcc taatcacagt taatcatgtt
      961 cggcccttca tgctgccggt tttgcagcac tactacggcg tcggctgact tctgccgccc
     1021 catcccggcg cctcacggca ccggtagcac actggcaggg cgacagatct cccggggtaa
     1081 ttcacgcgac tttcctgctt atgcctgtcg gctttacgtc acagcgttcc gtgtgagtat
     1141 cgggctttgg tgatttgtgc caccttaccc tgctgcgccg ccttaaccgc ttcctgttcg
     1201 tcaggccagc attttgcatc cggcttcctt cagatttcac ctcgcgatga acacccttgc
     1261 cttccgctaa cacttcccct caccgggtgt gtggaggact ttcacctctt agtcgttctt
     1321 cagccaccac agttgaagaa cagcgccagt cacggcgcta cgcgccatgc ccggcgcaca
     1381 tatgcaacga gcgccgatgt aaacggcacc cgtaatattt tagctgcagg gcacgccgta
     1441 cttgcctgta aagagatggt gcagtcaggc cttttgttga agcaggagcc caccgaaatg
     1501 attccggcaa tagcctgatt agcaggaatc cacatccttc tgggcgtgga ggatgacaat
     1561 ctaaaacaag ttaataaaaa ataccgataa tcgcagagtg aaaaacaaaa ccaactatca
     1621 ggggtaaagc agtgcgtttt accacttcga agggagttat atttgctcca ctggaaaccg
     1681 ctataaccac gccagatacg ggagacatcg cacgccccat atgggaagcc tgctgcatag
     1741 gcaggatcat cgatatggca ttcacgccca tgctggcagc gatttgtggt atcagttcaa
     1801 caaaagccag aaatggcgca ttgcccgatc ccataatgac agccgcagcc agtgtgacca
     1861 gcgcaaaaac gatgcccatc gcaaaagggg gtaacccaac gtgctctgcc atcaaaatta
     1921 attgatctat cgcgccaatg cttttaatcc catgagcaaa cacaccagca gccaccagta
     1981 atcccaccac gccggtaaat gctgtcccca ttcctttcag gaaatgggta aacccgtcac
     2041 atacagcttt cagatcacat ttacggaccc attcgatcaa catgcaaatt gccattgaga
     2101 taagaacaat cgtaatgata ttgagattaa ttccggtgac aaaaatctcc gatgagccaa
     2161 cggccatcag gatcggtagc ataggtagca gagcataata agccggcgta ctgcctgatt
     2221 ccatgaccgt tgtcgtacca atttcatgag gtaatgttcc tgctttacga tcacagtgtt
     2281 tctgccagaa aaagtggctg ataccgacaa ttagaacagt cgcaagagca gcaggtccct
     2341 gatgatagac aacatactcc accacatcca tatttaccgc ttcagagcct cgtatggcgt
     2401 caatggctgt cggtgtgtac gccacaccaa gagaggtggc aattacccct gcggcagacg
     2461 ctgctgacag cccgagtccg agcataatag gaaacatggt tcccatcagt aaaacagcaa
     2521 gtccggtagc tgaggggata gccagttgaa gaagactggc gaaaagataa gagaaaaaaa
     2581 gcagaatata gggggaacgt aatgtccgta gtggccgggt taccacacgg acaaccgctt
     2641 cattcgcacc gatatggtcc atgtaatgcg caaatcccat cagggtcata atcatcagac
     2701 cgagatcagc cgcacgggta ctaaataaat tgcgcataac ttcgaaagga tcaagaaaac
     2761 taattcctgt accggacacc cccttcggta gcacggtgcc ccaaccggta aaccaggtac
     2821 aaatcatcaa tgtaagcccg gcaataaaaa gcacgggttc tgcacgatat cctttcagga
     2881 ttaagcgagc aactatcacg ataacaagaa gggcgataac aaccttaatc atgactcacg
     2941 attctctgtg gtaaattcat taaatgtctc ctgaatgacg gcaacaacca catcacttgc
     3001 cgcccgaagg gaacggactg gaagatattc atagatagaa tggaaattat gtgcgccggt
     3061 aaaaatgttc gggcagggta atcctttttg tgaaagaaca gcaccatcat atcctccacg
     3121 catcgccacc ggtgtcggag taataccaca tcgttgatag gcacggaggg caatgtcgat
     3181 gggataatgt acctttccct gcagactgtt taaaacattg gcgtaccgat cgctcagttg
     3241 gcaggtcacg cttccttctc cccataatgc acaagcactt tctgcaagct ggcgtacaaa
     3301 tgtcttccgg gcgtgatatc cttcctcgct gaaatctcgt atatccagtt ttagtacagt
     3361 tctggcactg tttccctgta actgtttcac ccaataatag ccttcgcgtc cttctgtata
     3421 ttcaggcgct tctcctcctg gcaacatcga aatgaatttg tgagccatca aaagagagtt
     3481 cttaagcttg cctttcgctg acatagggtg tgcagactgg ccggtaaaga taatttctgc
     3541 atcacccgca ttccagtttt cgtaaacaaa ttctccaata ccgcagcaat ccagagtgta
     3601 gccaaaatct gcgccaaact ctgaaacatc gaaggctttg gccccccgta acccctgctc
     3661 ttcatctggc acaaacccaa ctttaacttc tccatgcctg atttcagaat gagaaatcag
     3721 aaattgaagt gcgttcatta tggccgccag cgccgcttta tcatcggccc ccagaaggct
     3781 ggtaccatct gtcactataa ggtcatcgcc aatataattt ttgagctccg ggaattcact
     3841 ctcacgcaaa taaatattca gttcagggtt cagacagaga tcgcccccat tataaggtaa
     3901 tcgttgcgct tttgtatcgg ctgtctgttc ggcactggtg tccagatgtc caaaaaagga
     3961 aacgacgggg attgtttcgt cagtatttgc agggagtgtt gctgttacga tagcattctc
     4021 tctgagaata atatcctcca tacccaatgc ttcaagttca ctcaccaaca atttcgcaag
     4081 tttcatctga ccaggagatg aaggcatgat cccttttgcg ccgttttctc gtgaggtagt
     4141 ggtattaatt tttgtataat tgataaagcg ttcgacaata tccattgaga tgccttcttc
     4201 aacattttcc agattttagg gacagtttat taacaccttc tgactctgaa gaacgtgaat
     4261 ttatgctatg ttcaggctgt catttttcca agagagaaat gataattatg aagagccttt
     4321 gcatgattga acataaaata cagactccat tacagtatgg cgttttttta gcttgtgatg
     4381 attaatgccg ttacaccctg aaagacgatt tagtgatcaa atcatctaaa aataattaat
     4441 cataatggat gttaaaatcc atgatctaac tcaaattaca aaccattaaa gcggaagatg
     4501 gagtggaaac attacgtttt ctactctatt ccaagattta tcaattagaa gtgcaataac
     4561 attcccgtgt aaaaactctt atcttaataa gaatcaccat gagtgactat atctacaacg
     4621 ctggaaaacc tagcagagat ataggttttc tatggtggta aacaggttgt ttttctgaag
     4681 gatgtgacca aaccgggatg ctattataaa atagttctac ttaatcatga tttaaaaaat
     4741 cagcattaat aataagtttg attgcggaa
//
LOCUS       DARNVM010000253         4597 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.253, whole genome shotgun sequence.
ACCESSION   DARNVM010000253 DARNVM010000000
VERSION     DARNVM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.253"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            31..1005
                     /gene="hemB"
                     /locus_tag="SI621_004560"
     CDS             31..1005
                     /gene="hemB"
                     /locus_tag="SI621_004560"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEI4067176.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1045..1896)
                     /gene="tauD"
                     /locus_tag="SI621_004561"
     CDS             complement(1045..1896)
                     /gene="tauD"
                     /locus_tag="SI621_004561"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HEI4067177.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWSSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(1893..2720)
                     /gene="tauC"
                     /locus_tag="SI621_004562"
     CDS             complement(1893..2720)
                     /gene="tauC"
                     /locus_tag="SI621_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HEI4067178.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWAVAAL
                     HLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2717..3484)
                     /gene="tauB"
                     /locus_tag="SI621_004563"
     CDS             complement(2717..3484)
                     /gene="tauB"
                     /locus_tag="SI621_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HEI4067179.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESCELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVAFGLQLAGVEK
                     VQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFITMREYVLSRVFEQREAFS"
     gene            complement(3497..4459)
                     /gene="tauA"
                     /locus_tag="SI621_004564"
     CDS             complement(3497..4459)
                     /gene="tauA"
                     /locus_tag="SI621_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEI4067180.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1039 a         1340 c         1203 g         1015 t
ORIGIN      
        1 cccctcgatt ccacaaacat caggcagacc atgacagact taatccaacg ccctcgtcgc
       61 ctgcgcaaat ctcctgcgct gcgcgctatg tttgaagaga caacacttag ccttaacgac
      121 ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt tgaagccatg
      181 ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg catcgccaac
      241 gccggtattc gttccgtgat gactttcggc atctctcacc ataccgatga aaccggcagc
      301 gacgcctggc gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa gcagactgtg
      361 ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat acacttctca cggtcactgc
      421 ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt aggcaagcaa
      481 gccgtggttg cagctgctgc aggtgcagac ttcatcgccc cttccgccgc gatggacggc
      541 caggtacagg caattcgcca ggcgctggac gctgcgggct ttaaagacac ggcgattatg
      601 tcgtattcga ccaagttcgc ctcctccttt tatggcccgt tccgtgaagc tgccggcagc
      661 gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg tgaggcgatt
      721 cgtgagtcac tgctggatga agcccagggc gcagactgcc tgatggttaa acctgctgga
      781 gcgtacctcg acatcgtgcg tgagctgcgt gaacgtactg aattgccgat tggcgcgtat
      841 caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc tatagatgaa
      901 gagaaagtcg tgctcgaaag cttaggttcg attaagcgtg cgggtgcgga tctgattttc
      961 agctactttg cactggattt ggctgagaag aagattctgc gttaatgttc attatgccgg
     1021 gttatccaac ccggcatttc ccccttaccc cgcccgataa aacggtttat ccccaaggat
     1081 cgtcgcccga tgcataatcc gccgctgtgg caggtaatcc gcattggcat agtgctgagt
     1141 cacgcgatta tcccaaatcg caatatcatt cggttgccag cgccagcgca cctgaaactc
     1201 cggtttggtg atatgggcaa acaaaaaact taacaaggct tcgctctctt tctcgctgac
     1261 atcaacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac cgctcaccgg
     1321 atgcgttcgc accaccggat gtagcaacgg cgggtttttc gcgaccgcct cgcgccagcg
     1381 ttgatgctcc tcctcggttt tgcggtattt gtattccggg aacgatttac ggaaatcatg
     1441 ctccgcacgc agcccgctca gcagttggcg gaagggaaca gagagcgcct cataggccgc
     1501 aataccgctg ctccagagcg tatcgccgcc ggtcgagggt aactcttttg ccgccagaat
     1561 cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt cgttatccgg
     1621 cggattatcg ttatgagtat ccagcacgat gatctcgtca accccttcgg catgagggta
     1681 aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt
     1741 aatagcttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt aaagctgttc
     1801 aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg cgccaatata
     1861 cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg ccagggcgtc
     1921 aggcggcgct gtaacgcgcg caaacccagt tctaaaagaa aagcgataat cgcaatcacc
     1981 gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg aaccataaat
     2041 cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca gcccacaccc
     2101 agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac
     2161 agcacctgcg cacgactggc acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg
     2221 cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa aaatcgctaa atagatcagt
     2281 aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa atacgccagc
     2341 ggcggcaccg gacgataaag ctcgattatc ggatccagaa tgccgcgcac cgtcgggctt
     2401 agtcccatgg cgatcccgac cggaatgccg atcaacaccg ccgcaagtag cgccagcaca
     2461 atgcgcgtca ggctggctgc aagatgctgc cacagggtgg cgtccataaa tccctgcggc
     2521 ccggcaatgg tgagcagttt tgccagtacc tgttgcggtg gcggcaaaaa cagcgggcta
     2581 atcaggtgca gcgccgccac tgcccaccat acagtgagta aaatcgctaa cgtgccaatg
     2641 cttaaggtca cctgacgcga gagcggccag cgccatttca gccgatgcga atgcagtttt
     2701 tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg cttaaaacat
     2761 attcgcgcat ggtgatgaat tgtggatcgg acttgatgct gcggctcgac tctcccgcaa
     2821 caaagcggcg agcaaagttg agcggcagcc gctccagcac gcggccaggg ccgggtgaaa
     2881 gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta atcaacagca
     2941 cctgcttgcc cgtctcctgc cagagtttca gcagcagagt ttgcatctga tcgcgggtga
     3001 aggcgtccag cgcgccaaac ggttcgtcga gtaataacag ctggggattc gccgccagcg
     3061 cgcgggcaat ccctacccgc tgacgttgac cgccggaaag ctgccagata tagcgttttt
     3121 ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagg cgctgtactt
     3181 tctctacacc tgccagttgc aggccgaacg ccacgttgtc taccacatta cgccacggca
     3241 acagcccttc attctgaaaa accacgcccc gctctgcgcc cggtccctca atgcacttac
     3301 ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga ttcagcaagg
     3361 tggttttgcc gcagccggac ggccccagca ccaccagtag ctcacagctt tccagcgtca
     3421 ggttgatatc ttccagtgcc ggtttgccgc cataatcggc gtaaagatga gagatttgca
     3481 gcatctgcgc ctccttttat tgcacgaagc gcgaggtcac gtactggctg taatcattcg
     3541 ctacagccgg gactttgccc tgctctttca aaaactgcgc ggtgtcgatg atcgctttgt
     3601 tcaccggtcc ggtcagttct gccgtttgct gctgcggcgt cagataggta ttccccttca
     3661 ccagccccgg aacgtcacct tcaggtacgc cgcttaaacg tgccagtttg ctgatgtttt
     3721 ccggctgttt cagccacgcg tctggattag caatgtacgg ttgttgagca tcgatggcgc
     3781 ttttagcgaa cgctttcacg acctctggat gtttctcggc aaaatctttg cgcaccaccc
     3841 agacgtccag tgtcggcgcg ccccactgcc cgacctgttc agaatcggtc agcaccttgc
     3901 cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca atgtctcccc
     3961 gttgccatgc ggcgataatc gcgggcggct gcaggttcac tatctccact tgtccgggtt
     4021 ttatacccca gtgtttcagc gccgccagca ggctgtagtg ggtggtggag ataaacggta
     4081 cggcgatgcg tttgccaatg agatcttccg gtttgctgat agttttcttt accaccagcg
     4141 cttcggagtt acccagtttt gacgccagta agaagacttc aatcggcacc tgttggctgg
     4201 ctgccaccgc taatgggctg gaaccgaggt taccgatttg gacatcgcct gaagccagcg
     4261 cccgcacgat gctggctccg ctgtcaaact tacgccagtc caccgtcgcc ccgctctctt
     4321 tagcaaaggt gttatcagcc tgagccactt tcgccggttc ggcggaggtt tgatacgcca
     4381 cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt
     4441 tacgcgatga aattgccatg attgtctgct cccctgtctt gttataggag cagtattcag
     4501 gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt attcttagca
     4561 aaaaagcggc aaaggaaaga ttaaatatgc aattaca
//
LOCUS       DARNVM010000254         4527 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.254, whole genome shotgun sequence.
ACCESSION   DARNVM010000254 DARNVM010000000
VERSION     DARNVM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.254"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(438..716)
                     /locus_tag="SI621_004565"
     CDS             complement(438..716)
                     /locus_tag="SI621_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067181.1"
                     /translation="MSTLNTRKDRDRNGKAKLHNHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(768..989)
                     /locus_tag="SI621_004566"
     CDS             complement(768..989)
                     /locus_tag="SI621_004566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter ATP-binding protein"
                     /protein_id="HEI4067182.1"
                     /translation="MTRNRAERRHHMARLKKSAAVIALQAMEAKKHQVFAFVLPVFVL
                     VGCVGIDAIIMVPAYRKFVQKHAMRIKGK"
     gene            complement(1074..1364)
                     /locus_tag="SI621_004567"
     CDS             complement(1074..1364)
                     /locus_tag="SI621_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4067183.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRAS
                     GLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1365..1676)
                     /locus_tag="SI621_004568"
     CDS             complement(1365..1676)
                     /locus_tag="SI621_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEI4067184.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1905..2813
                     /locus_tag="SI621_004569"
     CDS             1905..2813
                     /locus_tag="SI621_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEI4067185.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMILRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QYRVIAVQYRLAPEQTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2981..3895)
                     /locus_tag="SI621_004570"
     CDS             complement(2981..3895)
                     /locus_tag="SI621_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEI4067186.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKTLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQLDISARNKYRPAFDKWNELV
                     DKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LLEKRLADGREIEKLFDQQGYFSCDSLTAKELMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(3908..4527)
                     /locus_tag="SI621_004571"
                     /pseudo
     CDS             complement(3908..4527)
                     /locus_tag="SI621_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1217 a         1046 c          932 g         1332 t
ORIGIN      
        1 aaaccgtatt catcgccatt accaatacct tacatatatt actcattaat gtatgtgcga
       61 aaattacgcc gaaaaatcat ttaaaaaaag cggatttttg catgctgttt tgtttttgcg
      121 agacggcatc caaagataat gtaacagttg cagttatttc aattgttact caaaaatggt
      181 ttagtaataa cccaggatgg tttattactt ttaattaagc ggggacacat ctacagaaaa
      241 gaaacaacaa ataataagga tgaattatgg gtgctataaa tattttatat tcaagaatat
      301 ttttttgatt gggtgttcat ttgcttcatc aggatggtgt acccgaaaaa gtcagcagct
      361 ttaccgcacc agggtttaag gtgaaatcta ccattcataa ctgaccggtc agtgccgcac
      421 caaccggtct ttaattgtta gtagtagtaa atttgtggat aacagtccgg gaaccagacg
      481 attccttctg gatcatcccc ctgcattacc cgatgacatt ggcgtcgatc atctgcgcga
      541 cggcgaagcg tagtatagag ccgcttccat tcatgatcgc ccggtgtact gtagtttacc
      601 caacaatcca ttgaagggtg acgccattta cctgtaagtg ttgggatccc tgaatgatta
      661 tgcagcttcg ctttaccatt acgatcccta tctttacgcg tattaagcgt ggacattttt
      721 agtctcctgt taagagttct aaaaatgtgc ttccttaaaa tagttcatca ttttccctta
      781 attcgcatag cgtgcttttg cacgaatttc cgatacgcgg ggaccattat gatagcgtcg
      841 atgcccacac atccaacaag aacaaataca gggagtacga aagcaaatac ctgatgcttt
      901 tttgcttcca tcgcctgcag tgcgataacg gcagcggatt tttttaatcg cgccatatga
      961 tggcgacgtt cagcacgatt acgggtcata gtaaaactcc tgagcaggcg gcactatgcc
     1021 gctttcatag gagttctgaa tgctggtgct ttctgtatgg cataaccatg cctctaacgg
     1081 ttaagtgcct gcagcacatg ttgcggatca ttggcaatcg ctcgcagcaa agcctttgca
     1141 ggcccagtcg gtgaacgtcg cccctgctcc cagttacgca acgtgtctac gcttacggaa
     1201 ataagttcgg caaatttaga ttgtgacaac cctgaagcgc ggcgtatttc tttgattttc
     1261 atagcatcaa cttcaaaagt ccgtgaaggg tgtcgcgttc cttcaataat ttcattcatc
     1321 tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat ccatttacca cctcgtattt
     1381 aacaaccgga gcattgcttt ttcctgggga gacagatcat ctttaatacc cttacgataa
     1441 ataagcagta atctgatctc ataatgttta acctgatgaa agtaaataac cctgacacca
     1501 gcacgtttcc cctttcctcc tgacacccat cgaacctttc ttaacccacc agtttcaggt
     1561 attacctcac catagtcagg attcaaggcc aggaaaaact gaaaacgact gaattcatcg
     1621 tcggtcagca gtttttgaac atcttcagta aaaatttccg tttcgatgaa gagcataatt
     1681 atacgccatt ggcttagagt aacaatcctt tttgtacgtc attgacgtac ccttgaaacc
     1741 actagcttac tgatagtgct ccagcaaaac cacgtctctg ccgggtttgt ctttacattt
     1801 gcgaggcgct ttccagattt ttcacgcccg tgaatttccc tgtatccatc cagcaaaaac
     1861 gggtgtataa aactgtacat tcatacagca aaatgagttc tactatggca ctggaaaaag
     1921 gaattgcaga gttagtcgaa gagtttatcg ctgcgggtcg cccctcatcg cgtcaacaaa
     1981 gtattgcgca gcgcagggag ggctatatcg ccagcgctgt ccttgcgggt gagacagaaa
     2041 cgcgggtaga tattcagact atcgaacttg aagggatgat cttacgaata gtgtcaccgc
     2101 tgaatgcccc gacattactc ccgacgataa tttattacta cggtggctgt tttgttagcg
     2161 gcgggttcgc aactcacgac aaccagctgc gccagttagc ctattacggg caatatcggg
     2221 ttattgccgt ccagtatcgg ctggcaccgg aacagacttt ccccgccgcc catgatgatg
     2281 cgcaacgagg tgcagaatta gtctggcaac acgccgaacg actcggcgtt gataaacaac
     2341 ggataactct tgccggtgac agtgcgggcg gacatctggc gctggtaact gctttgcgat
     2401 taaagcgtgc tggcgagtgg caaccggcac aactcatctt aatttatccc atgcttgatg
     2461 cgactgcgca tttcgaaagc tacatacgca atggtcacga ttacatcatc acacgcgaca
     2521 ctttactgag cggctttgag atgtatctgc caggcattga acgcaggcat cctgaagcca
     2581 gccctctttg gcgcgatgat ttcaatggct tgccacctgt acatatcatt accgctgaat
     2641 acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg catgacaggg caaggagtgc
     2701 aatgcacctg ccaacgctac cttggcgtta tccacggatt ttttcagtta ggtggtatca
     2761 gtgaggctgc gcgatcggcg ctgcgggatg tggcctggag agcgggaagg taactcctga
     2821 gcagcggcac tatgccgctt tcacaggagt tctgaatgct ggtgctttct gtatggcttc
     2881 gccatgcccc tcgcggctaa gcgcctgcac acatctctct ttgaatgtca tttttgcaat
     2941 gcattggccg aattctggta attctaatga gttttactct tcagactccc atccctacgg
     3001 tatacgagtt agattcatga atgaaatcga catttttaaa aaacgaatct tgtaaaacat
     3061 ccgtgttgat attaccttgc aacccaacat taaatttgtt agagagatga agaactcgaa
     3121 tatcaaaaag gtcttttaac atcattaatt ctttggctgt taatgaatcg caactaaaat
     3181 atccctgttg atcaaaaagc ttttctattt ctctaccatc ggcgaggcgt ttttcaagga
     3241 gtgtttgcaa catcgagttg ccaaggacaa tggatatcgt acgatctttc gcagatttac
     3301 accccatcac aacggcaaca ttcgtatccc cctcaggagt ttcatttatc gccgtcgcta
     3361 aaatggtggt caaacacgca aacggaactg tatttctttc gtgatttaat ttatctacca
     3421 gctcattcca tttatcaaaa gccgggcgat atttgttgcg cgcagaaata tccaactggt
     3481 taaacaattc agccatgcga ttttttatca tctcgatatc cggcgaacca gaaagacttt
     3541 gcgcataata cggtcctgat gatgttgcat acattaaagc cgatcccacg cctgtcgcca
     3601 tgatgggcgc agctacggtt ttggcaaggg cgacgctgcg atggctatgc gcttcacagg
     3661 ctaaaggaac atattccact gactgcggta attccttaca tgcctccctc atcattgcgg
     3721 tatgctttac aaacaacgtt ttttcactgc ccaaaaaaac tttctgctta attgcactaa
     3781 gaaaacgtaa atcaaaaatg attgtttttg gtttattatt ttttggcaca tttcctatta
     3841 tcccgctaag aacctttctt gctgcatgga gatttttttg cacagcacat aacacaattg
     3901 acgaagcctt ctctactgcg ttattgattt taattttaaa atctgcatga tatacgttga
     3961 cgggcaatgc aagtatatct tcccctacat ctttggctga gtatttattt gtgatcgatg
     4021 gtcgtgaatc cacgagaaaa cttagctgca aattatgctc tatattacca gctgtttttg
     4081 tagtgtgatt aaacatagag atgccacctc tcattctttc acacataccc tcaacttttt
     4141 cacttcgttc ttgaggcaat ttggctttaa cgttaatttg atgattttta cccgtgcatt
     4201 ccttaactac aagaggaatg ttatctaaag tgccatacag gctaataacg tcaccaggat
     4261 ttttatcatg taagtaagtt tgattaatac tcgaagcatt cacaaagagg tcaatattat
     4321 aactgtttat gacttcacct acatcaaatg tgtagtgctt actctggagg ctgccatcct
     4381 tgtttaattg tatatcaatc aatatatccc cggactttgt caccatatct ttgccataaa
     4441 ccatttgcat agccatttcg aggagatttt tatctaactg attgcgggcg ttgagtaagc
     4501 gactaccaaa taatttatat actgttc
//
LOCUS       DARNVM010000255         4419 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.255, whole genome shotgun sequence.
ACCESSION   DARNVM010000255 DARNVM010000000
VERSION     DARNVM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.255"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..359)
                     /locus_tag="SI621_004572"
     CDS             complement(<1..359)
                     /locus_tag="SI621_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HEI4067187.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRP"
     gene            complement(371..877)
                     /locus_tag="SI621_004573"
     CDS             complement(371..877)
                     /locus_tag="SI621_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067188.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            complement(881..2101)
                     /locus_tag="SI621_004574"
     CDS             complement(881..2101)
                     /locus_tag="SI621_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HEI4067189.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITVLHPEDENGDVRLVNPENWKELAVGH
                     LQNVRRGTGVQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYKQTEPGKAQQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEVGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRPNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2116..2850)
                     /locus_tag="SI621_004575"
     CDS             complement(2116..2850)
                     /locus_tag="SI621_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEI4067190.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADIVRKNDEQWRKHSKTISRELRNLVNSAPPGQVMKS
                     VVAEQVKYIKSLPLEAADRVYDIQNRAIEAVVTGGRAEHFAKEIAASGDIAKSRADLI
                     ARTELGRATGALDQARALAIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGEFPNCRCYKEIVFPTSHSYPA"
     gene            complement(2741..4207)
                     /locus_tag="SI621_004576"
     CDS             complement(2741..4207)
                     /locus_tag="SI621_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HEI4067191.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWPYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGTLEAQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPLSNQPTQDSAGGRRHRKWSLRWFK"
     gene            complement(4207..>4419)
                     /locus_tag="SI621_004577"
     CDS             complement(4207..>4419)
                     /locus_tag="SI621_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HEI4067192.1"
                     /translation="IISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPDGTRSPN
                     LADSVMINYAPMNSALNIWELLGRQA"
BASE COUNT          933 a         1261 c         1115 g         1110 t
ORIGIN      
        1 atcgggcgtc cgacctgctg agcagcctgc aattctatga ccgtccagcc aagttccatc
       61 ccccacaggt tcagcgggtt accggatttg ccgatatcca cgtttacgcc agcaatagcg
      121 gttgagtctt tgcctaccca gtttttgcca ttcggatttg caccagtacc cgcagcggcg
      181 aagctggtat tcgtccagct ggaaatgtca tctgcgatag agacatcttc acgcaactga
      241 atatcgcggg tccaggtgta ccccaccagt ggcaggttca gcgtctggtc gagtcgctcc
      301 agctctccga tgagaaaggc accagagctg tcaacggttg cctgatcaaa agtaatcatt
      361 cgtctgttcc ttaaatcttc caggaaattt ctgcattgcc gtcagcatca ccggcacctg
      421 tgaattcagc gttggtcagc accacatttt tgccactgac tgacgtggac atgaatccac
      481 ccagcggcac tttgatggat tcatcagtgg agacgacaac gtataccggg tcgccttttt
      541 tgatggtgct ggcatcaaaa tcagaaccga gattaacggt cacgtagcca cgcttcatgg
      601 cgtcgccagg gaagttcttg ccactcccca cctggcgaac catgtccggc tgcgaagtgg
      661 tcggataagg gcgcacgtag atccccttca ccttgtctgc tgtatcacca tctgccagag
      721 gcacgaaaaa accgtcatca tcgtatttac cagccagccc ataggcagcg aaggcgttat
      781 cggatttaag gaccaccggt tcgacggtta agtcctgcgg gcgagagaca gccccggcaa
      841 tgccaacagg catccggtac agaaatacat tattcatttt ttacccttta cggtttgccc
      901 agaattcagc gttttgtttg ttcagggaag cgatactggt catgcccatg tttgggcgct
      961 gtgcatcgcc ggtggtggcg cgggtgtttc gccctttggc aatctcagac acggcattaa
     1021 acgccatgtc gaccgattgt ttcggtaatt tgcggatatc cgcatcaccg actatctggc
     1081 gaaccagcgt tttgtcagca gaagccagaa cctcgcgttt gaacgcggtc ggtttcatct
     1141 tacgactcag atcgataccc ggaacgataa cttcggcacg ccaggctgag tcaccagtaa
     1201 tcgtggtttc ctcttcatcg tcctcgccgt caccggtcgg attatcgtca ggcttattgt
     1261 cgttatcgcc cgtcgcattt ccttccagct tagccagcag ggctttcagt aatgttttga
     1321 ggtcatcatc actgtcgccg gttggacctc cgcccacctc tggtgctttg tccggtagcg
     1381 gttgctgcgg ggacaggttg atgttgagat taacgccctg cggcaaatcc ccctcatctc
     1441 ctgtaaccga tgcgggagcc gactccacca gttcgttcat ggtgtcagcg tcacccgttt
     1501 tgatggccgt gcgcatgcgg gtccaccagc ttttcttttg atttgccatt gtgtctctgt
     1561 ctccaattgc acaacgattt ccggctctgc ctttagggac aagagccaca tggtttccgg
     1621 taatatcaac ctgctgagcc ttacccggct cagtctgctt atattccgcg tcatagccac
     1681 acgacacttc gcgcaggcca tcttcgatca gttgaatggc gttttcgtct ttgacgataa
     1741 ggtcagccag catcaaatca gactgcacac ccgtcccgcg ccggacattc tgaagatgcc
     1801 cgaccgcgag ctctttccag ttctctggat tcaccagccg cacatctccg ttttcatcct
     1861 cgggatgcaa caccgtgata ctcattcctt cgaatgaggc aagcgtggcg ggatggaata
     1921 cctgctcagg agaacgcgtg acgactattt caccgaactt atcgggtttc agttttggta
     1981 ggtcatcagc accatagagc tgcttacctg ttcgtcctat cggcacgtct ttgcacagca
     2041 acgagccgtc agccaactga tagcgggttt cccccagccg ggtattgaaa aaatatttca
     2101 tgtgttacct gcgattcagg cggggtaaga atgggaggtg ggaaaaacaa tttctttata
     2161 acagcgacaa ttcgggaact cgccagcgtg acctgtcatg ccgtcaagcg ttggaggttt
     2221 gccccattcg acaaatttac cttccatttc ccgatgagaa tgcctgacat caccatcttc
     2281 ggctgtacgc cagatataac cattcgaacc aattgccagc gcacgcgcct gatccagcgc
     2341 gccggttgca cgtccaagtt cagtacgggc aatcaggtca gctctggact ttgctatatc
     2401 acccgatgca gctatttctt tagcaaaatg ttccgctctc ccaccggtca caacagcttc
     2461 aatcgcccga ttctggatgt cgtacaccct gtcagccgcc tcgaggggta gcgatttaat
     2521 gtacttaacc tgttcagcaa cgacggattt catcacctgc cctggcgggg cactgtttac
     2581 cagattgcgt agctcacggc tgatggtttt gctgtgttta cgccactgct catcattctt
     2641 gcgcacaata tcggcggtaa agttttccgc gacctttgtc gcccaagggg tgatgatttc
     2701 actgtagcgt tccagcgcct caataatttc cgtgatactg tcatttgaac catcgtagcg
     2761 accatttacg atgtctccga ccgcccgcgc tatcctgcgt aggctggttc gatagcggat
     2821 ttccgcctga cggttcctgc ggttcgtcat caggttcgcc gatgccgggc ggcgcttcgt
     2881 cttcggcatt ctctatgtcc tcgtcggtaa tggatgcccc gatgccggtt acatcagaat
     2941 tttcgcgcaa atcggtcata gcggctttca gtgtcatcag accatcaccc agcgccgtac
     3001 tgattgcgtt ggtggtgttt aacgccaccg ttgagcgatc gacatcagac atttgccaga
     3061 gcgggttaaa ctcaaatgtg aaatcatccg ggagcggctt gccaagttcc gaacgatgca
     3121 tgatgtccag tatccgccgc accggaagac gtaaacgcct ctcctgcaac gaacttaccc
     3181 ggtcgtaata gttggcaagg tctgcatcgc cggtagaaaa tcccttcggg gactgtccga
     3241 acaaccgcac cagtgggata ccaacagcgc cactaatctg ttctgcaaac tgtgaaagga
     3301 tgtcatccag accactgaag ctgtactgat gggtttcaaa cttatcccgc gagtccatga
     3361 gcgtcatgcc ttcattgctc tggaactgtc gaatcaggtc gatattcttc agcaacgctt
     3421 catacgcagg accaccaagt gcgataagct cgcgtagctt ctccacgctg taggtacgca
     3481 aatgcgcctt gtagaccagc tgcgccgcgc cgacagtagc gctgtcgaac gcggtaagac
     3541 gatcccagat acgctctaca accgacattc cccattcgtt ttcggtcatc ttctgctgga
     3601 atggcagcgt caccccgtcg aagcgaatca ggcggctgtg atggatgcgc caggccggga
     3661 tgcccgttgc agtggtcacc acgtcgtaaa actcaggttt acccaggtcc ggccccatct
     3721 ctttaatgcg gcgggtcagc acagggttaa tcatccagcg gtcgagcggg agaatgccct
     3781 taaacttgcc ttctccaatg gtttcgagcc gcaacggggt cattggtgcc tgcccctcaa
     3841 tcatgatgaa gccaaccgcg ccgccgtaga gacgcgacca tttcagcacg tcgttcagcg
     3901 catcccagat ctgcaactca tccagctgcg cttccagggt gccacggtct ttggcgtcaa
     3961 tctccgaagt gatgcgaatg cctttccggg tcatatcgtc cgggatagcg tcgaccgctt
     4021 cgccgatgat ccaggacgaa cgatagggcc attccaccag catgcggttg cggctggtga
     4081 agttagcccg gtaggtcgat gctgagtgct ggttaggtgt ctgcatccct acgcgggcga
     4141 taaaattctc ataaccatca gctgtggcct gcgcagttcg ccgcagggct tgtttgtttc
     4201 gtgccatcag gcctgtctcc ctagcaactc ccagatgttc agggctgaat tcattggggc
     4261 atagttgatc atcaccgagt cggcaagatt tggcgaccgg gtcccatcag gctgtttatc
     4321 aataacgatt tttcccacac cattaatgga ataggtcggc tgcgaaagct cgatgatgag
     4381 tttatctttg agtgccatgc tactgctgat tgagatgat
//
LOCUS       DARNVM010000256         4313 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.256, whole genome shotgun sequence.
ACCESSION   DARNVM010000256 DARNVM010000000
VERSION     DARNVM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.256"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            54..1211
                     /gene="acrE"
                     /locus_tag="SI621_004578"
     CDS             54..1211
                     /gene="acrE"
                     /locus_tag="SI621_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HEI4067193.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLSRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESAHINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQSDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADSASK"
     gene            1223..>4313
                     /gene="acrF"
                     /locus_tag="SI621_004579"
     CDS             1223..>4313
                     /gene="acrF"
                     /locus_tag="SI621_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HEI4067194.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGVLAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     "
BASE COUNT         1030 a         1061 c         1159 g         1063 t
ORIGIN      
        1 acgcgttgcc tttttgggta aataacgcgc ttttggtttt ttgaggaata gtaatgacga
       61 aacatgccag gtttttcctc ctgccctcct ttattctgat ctccgcggct ttaatcgccg
      121 gttgtaacga taagggagaa gagaaagctc acgtcggtga accgcaggtt accgttcata
      181 ttgtgaaaac ggccccgtta gaagttaaga ctgaattacc aggccgcacc aatgcttacc
      241 gtatagccga agttcgccca caggttagcg ggatcgtact gagtcgcaat ttcactgaag
      301 gcagcgatgt gcaagcaggc cagtctctgt accagatcga tcccgcgacc tatcaggcaa
      361 gttatgacag cgcgaaaggc gaactggcga aaagtgaagc cgccgccgcc atcgcgcatt
      421 tgacggtaaa acgttacgtt ccgctcgtgg gtacgaaata catcagccag caggagtacg
      481 accaggccat tgctgatgct cgccaggccg atgccgccgt gattgccgca aaagccacag
      541 tcgaaagcgc tcacatcaat cttgcttata ccaaagtgac tgcgccaatt agcggacgta
      601 tcggcaaatc gactgtgacc gaaggcgctc ttgtcactaa tgggcaaacg actgaactgg
      661 cgactgtcca gcagctcgat cctatctacg ttgatgtgac ccaatccagc aacgatttta
      721 tgaggctgaa gcaatccgta gagcaaggaa atttgcataa ggaaaacgcc accagcaacg
      781 tagagttggt catggagaac ggtcaaacct atcccctgaa aggtacgctg caattctccg
      841 atgttaccgt tgatgaaagc accggctcca taaccctacg tgctgtcttc cctaacccgc
      901 aacatacgct tttacctggt atgtttgtgc gcgcacggat tgatgaaggc gtccaatctg
      961 atgccattct tatcccgcaa caaggcgtta gccgcacacc gcgtggtgat gcaaccgtgc
     1021 tgattgttaa cgataaaagt caggttgaag cgcgccctgt cgttgccagc caggcgattg
     1081 gcgataaatg gttgattagc gaagggctga aatctggcga tcaagtcatt gtcagcggcc
     1141 tgcaaaaagc gcgtccggga gagcaggtta aagccactac cgataccccc gcagattctg
     1201 catcgaagta aggcaatctg acatggcaaa cttttttatt cgacgaccga tatttgcatg
     1261 ggtgctggcc attattctga tgatggcggg tgtactggcg atcctacaat tgcccgtcgc
     1321 ccaataccca accattgcgc cgcctgcagt ttctgtttcg gcaaactatc cgggcgctga
     1381 tgcgcagacc gtgcaggata cggtgacgca ggttatcgaa cagaatatga atggtatcga
     1441 taacctgatg tatatgtcct ccaccagcga ttctgccggt agcgtgacaa ttacccttac
     1501 cttccagtcc gggaccgatc cagatatcgc gcaagtgcag gtgcagaaca aactccagct
     1561 cgccacgccg ttgctgccgc aggaagtaca gcagcagggg atcagtgttg aaaagtccag
     1621 tagcagctat ttgatggtgg cgggctttgt ctctgataac ccagacacca cacaggacga
     1681 tatctcggac tatgtggcct ctaacgttaa agatacgctt agccgtctga atggcgtcgg
     1741 tgacgtacag cttttcggcg cacagtatgc gatgcgtatc tggttggacg ccgatctgct
     1801 aaacaaatat aaactgacgc ctgttgatgt gattaaccag ttgaaggtac agaacgatca
     1861 gatcgctgcc ggacagttgg gcggaacgcc agcgttacca gggcaacagt tgaacgcctc
     1921 gattattgct cagacgcggt taaaaaatcc ggaagaattc ggcaaagtga ccttgcgcgt
     1981 aaacagtgac ggctcggtgg tacgcctgaa agatgtcgca cgggttgaac ttggcggtga
     2041 aaactataac gttatcgctc gcatcaacgg taaaccggcg gcgggcctgg ggattaagct
     2101 ggcaaccggc gcgaatgctc tcgataccgc gaaagccatt aaggcaaaac tggcggaatt
     2161 acagccattc ttcccgcagg gaatgaaggt tctctaccct tatgacacca cgccattcgt
     2221 ccagctttct attcacgaag tggtaaaaac gctgttcgaa gctattatgc tggtgttcct
     2281 ggtgatgtat ctgttcttgc agaatatgcg agcaacgctg atccccacca ttgcggtacc
     2341 cgtggtgttg ttagggacgt ttgccatcct cgccgctttt ggttactcca tcaacacact
     2401 aacgatgttc gggatggtgc ttgccatcgg gctgctcgtc gatgatgcga tagtggtggt
     2461 ggagaacgtc gagcgcgtga tgatggagga taagctcccg ccaaaagaag cgacggaaaa
     2521 atcaatgtcg caaatccagg gcgcactggt gggtatcgca atggtgctgt cagcggtatt
     2581 tattccgatg gcattcttcg gcggttctac tggggcaatt tatcgccagt tctctatcac
     2641 catcgtttcg gcaatggcgc tttctgttct ggtggcattg attcttaccc cagcgttatg
     2701 tgcaacactg ctcaaacccg tctctgctga gcatcacgaa aataagggcg gtttcttcgg
     2761 ctggtttaat accaccttcg atcatagcgt taaccactac accaacagcg tcggcaaaat
     2821 cctcggttcc acaggacgat atttactgat ctatgcacta attgttgctg gaatggtggt
     2881 gttgttttta cgtcttccgt cttccttctt acctgaagag gatcagggtg tctttctgac
     2941 tatgattcag ttacccgctg gcgcgacgca agagcgaacg caaaaagtgt tggatcaagt
     3001 gacggattac tatctgaaga acgagaaagc gaacgtagaa agtgtcttta cggttaacgg
     3061 ctttagcttc agcggccagg cacaaaacgc cggtatggct ttcgtcagtc tgaaaccgtg
     3121 ggaagagcgt agtggtgacg aaaacagtgc ggaagcggta atccatcgtg ccaaaatgga
     3181 attgggcaag atccgcgacg gttttgtcat tccattcaat atgccagcca ttgttgaact
     3241 gggcacggca acgggtttcg actttgagtt aattgatcag gctgggctgg gtcatgatgc
     3301 cctgacccag gcccgtaacc agttgcttgg catggcggcg caacatcctg ccagcttagt
     3361 cagcgtgcgt cctaatggtc tggaagacac cgcgcagttt aaactggaag ttgaccagga
     3421 aaaggcgcag gcattaggtg tttcactttc tgacatcaat cagaccattt caacggcgtt
     3481 aggtgggact tacgttaacg acttcattga ccgtggtcgc gtgaaaaagg tgtatgttca
     3541 ggcggatgcc aaattccgta tgctgccaga agatgtcgat aaactttatg tccgcagcgc
     3601 caacggcgaa atggtgccat tctcggcctt taccactgca cattgggtgt atggctctcc
     3661 gcgactggag cgctacaacg gtctgccgtc aatggagatt cagggggaag ccgcgccagg
     3721 aaccagttcc ggcgatgcca tggcgttgat ggaaaacctt gcatcacaat taccagctgg
     3781 cattggttat gactggacgg gtatgtcata tcaggaacgc ttatcgggaa accaggctcc
     3841 cgctctggta gcaatttcct ttgtggttgt tttcctgtgc cttgctgcac tctatgaaag
     3901 ctggtcaatt cctgtctcgg ttatgttggt cgtgccgtta gggattgtcg gcgtgctact
     3961 ggcggcgaca ctctttaatc aaaaaaatga cgtctacttt atggtgggct tgctaacgac
     4021 aattggcttg tcggctaaaa acgctatttt gattgttgag ttcgctaaag atctcatgga
     4081 gaaagagggt aaaggtgttg ttgaagcgac actgatggca gtacgtatgc gcctgcgtcc
     4141 tatcctgatg acctctctcg cctttattct cggcgtatta ccgctagcta tcagtaacgg
     4201 tgccggcagt ggcgcgcaga acgcagtggg tatcggggta atgggcggga tggtttctgc
     4261 aacgttgctg gcaatcttct tcgtgccggt gttctttgtg gtgatccgcc gtt
//
LOCUS       DARNVM010000257         4295 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.257, whole genome shotgun sequence.
ACCESSION   DARNVM010000257 DARNVM010000000
VERSION     DARNVM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.257"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1559
                     /locus_tag="SI621_004580"
     CDS             <1..1559
                     /locus_tag="SI621_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4067195.1"
                     /translation="AKASETAAKNSQDAAAQSESAAAGSATAAANSQKAAKTSETNAK
                     TSETKAAASETEATSAATRAENAARIAEDAADPASVPPLPDIWLPLNDSLEAITGYAP
                     GYKTITIGSDEITMPVNGICQFSRASSATYIDKSGHITVAGNNVPRFEKYGLLIENQR
                     TNMFVNSFNPDAWNKSGGISVTSSTDEFEFKYGRFTVGSDIAGTTTGRNICTVAGNKG
                     IDVTGDDQYSKGPYVTASFRVRSDLNVRARIRFERYNSEGYTFLCDAYLSLQTHELQI
                     TGGNAQLLTANFEIDPGSGWIYFQATLKCLPEWGMVGTQLQIAADRAVGSFATGDWIE
                     VTTPQFEYGACATSFIITTTEPATRASDLCKFPLMKNMYTMPFTFMVEVHKNWFISHN
                     AAPRVIDSENHQFGGPFIMGFGSSGTISQDGYSYCDIGGANRRVYESCGVRDLVMGFR
                     VKADGMTCSFANKHISTETKTVWKYIREAAVIRIGGQTTTGLRHLNGHIKNLRFWNRA
                     LSDTQLKEYV"
     gene            1559..1828
                     /locus_tag="SI621_004581"
     CDS             1559..1828
                     /locus_tag="SI621_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067196.1"
                     /translation="MRDITLRFDNREQFNAIVYDSGLFSLEEENGVLVDVIGCIIDYE
                     EPENERCTGIDRGGFFVNMRIVDGSKNISSLMPFITTDQHVRTWA"
     gene            1841..2524
                     /locus_tag="SI621_004582"
     CDS             1841..2524
                     /locus_tag="SI621_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEI4067197.1"
                     /translation="MVTKTVIPGDITTLKGDISSINGKVSTLQTEMTSAKQDISSRYT
                     KTEVDNKLKNKVEVNDLESGRYGGDFYPLTGREAFYLWNLGTTTAAANLYLNPDPAIS
                     SVLRSTSSIRYKHSVETIDSEHAALIFRMRPVWYRSQCENDRHDWGFYGLIAEEVGEI
                     APQFVHWRPANEDDAPEAISSNGLVAEGVMYERLVVPLIHHIQKLTERVDELESELKL
                     LSTSRSDIG"
     gene            2535..2828
                     /locus_tag="SI621_004583"
     CDS             2535..2828
                     /locus_tag="SI621_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067198.1"
                     /translation="MDITPFLHALCAVAAQVLIGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            2848..2964
                     /locus_tag="SI621_004584"
                     /pseudo
     CDS             2848..2964
                     /locus_tag="SI621_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(3162..>4295)
                     /locus_tag="SI621_004585"
     CDS             complement(3162..>4295)
                     /locus_tag="SI621_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEI4067199.1"
                     /translation="IMGILGVDTYLPSIPDIAKEFRQNIPSMQLSIMVYTLCIGFGQL
                     IFGPLSDSIGRRRIMLSGAIIYCVTTYCLTLSTGLGTFLTIRIIQGLSISITLVTAIS
                     AVRDVTRGSVAATLYAIIITIEGVVPIASPLLGGIINDVWGWRAIFLMILGYSVVTLT
                     YVYFNFPETLSHKKRITYSLKSSFLTYKEISKQPRFYLPCLSLGLSFSLIYCYVTASP
                     FILMVKYGYSSTQFGVISSIIGGFLLLGAALSGKLLKKRSAPQILSLWLKLIASFIVL
                     CTPLITFVHHVNVFILFFIVLMFAGGMFESLYTYLTMSSQRTSLGATSALMGAASLVI
                     PSVIGGIGSFLIERNITIWIVYLLSIILIMIFMLRKYRRFTAA"
BASE COUNT         1332 a          828 c         1018 g         1117 t
ORIGIN      
        1 atgccaaagc cagcgaaact gcggcgaaaa acagtcagga tgcagcagcc caaagcgaga
       61 gtgccgcagc tggttctgca acagcggcag ctaatagcca aaaagctgca aaaaccagtg
      121 aaactaacgc aaagacgagt gaaacaaaag cagcagccag cgagacagaa gcaacgtcag
      181 ccgcaacaag agcagagaat gcagcccgga ttgcagaaga tgccgctgat cctgcttctg
      241 tccctccgct tcctgatatc tggctaccct tgaatgattc tctggaagcg ataacagggt
      301 atgccccggg ctataaaaca ataaccatcg gcagtgatga aataacaatg cctgttaatg
      361 gcatatgcca atttagccgg gcttcatctg caacgtatat tgataagtcc gggcatatta
      421 ccgtggcagg gaataacgtt cctcgttttg aaaaatatgg tttgctgata gagaatcagc
      481 gaacaaacat gttcgtaaat agttttaatc ctgatgcctg gaataaaagc ggtggtatat
      541 ctgtaacatc atcaacagat gaatttgagt ttaaatatgg acgtttcacg gtaggaagcg
      601 atatagcagg aacgacaaca gggcgaaata tatgcacagt tgctggtaac aaaggcatag
      661 atgtgactgg cgatgatcag tacagtaaag gtccgtatgt taccgcgtcg ttcagggtaa
      721 gaagtgatct caatgttcgc gcacgtatcc gttttgaacg gtataactcg gaaggataca
      781 ccttcctttg cgacgcctat ttgtcattac agacccatga actacaaatt actggtggca
      841 atgcccagct attaacagca aactttgaaa tcgatccagg tagtggatgg atatattttc
      901 aggcaacact gaaatgtctg ccagaatggg gaatggttgg tacgcagctg caaattgcag
      961 ccgacagagc tgtggggtct tttgcaacag gtgactggat agaagtaacc accccgcaat
     1021 tcgagtatgg tgcttgtgca acttccttca tcataacgac aactgagcca gcgactcgtg
     1081 catcagattt atgtaaattt ccgctgatga aaaatatgta taccatgcct tttacgttca
     1141 tggtggaagt ccataaaaac tggtttattt ctcataatgc ggcaccgcgt gtcattgatt
     1201 cagaaaatca ccagttcgga ggcccattta ttatggggtt tggttcttct ggaactatta
     1261 gtcaggacgg ttattcgtat tgcgatatag gcggggctaa ccggcgtgta tatgagtcat
     1321 gcggagtaag agatcttgtt atgggattca gggttaaggc tgacggcatg acatgctcat
     1381 ttgcaaataa gcatataagc acagaaacaa aaacagtatg gaaatatatt cgtgaagctg
     1441 ctgtgattcg tatcggggga caaacgacga caggattacg acaccttaat ggtcatataa
     1501 aaaacctccg tttctggaac agagcattgt cagatacgca gcttaaggaa tacgtataat
     1561 gcgggatata acattacgat tcgataacag agaacagttt aacgcaattg tatatgacag
     1621 tggcctgttc agtcttgaag aagaaaacgg ggttcttgtt gatgttattg gctgcattat
     1681 tgattacgag gagccagaaa acgaaagatg tacaggcatt gatcgcggtg gttttttcgt
     1741 aaacatgagg attgttgatg gcagtaaaaa catatcttct ttaatgcctt tcattacgac
     1801 agatcagcat gtaaggacat gggcttaatg gagtaagaca atggttacaa aaacagtaat
     1861 tccgggagac atcaccacgc taaagggaga tatttcctca attaacggaa aagtatcaac
     1921 gcttcagact gagatgacca gtgcaaagca ggatatcagc tccagataca caaaaactga
     1981 agttgataat aagctgaaaa acaaagtgga agtgaacgat ctggaaagtg gtcgttatgg
     2041 tggagatttt tacccgctga ctggccgtga agcgttttat ttatggaatt tgggcacgac
     2101 tacagcggcg gcaaaccttt atcttaatcc tgaccctgca atttcgtctg tactgcggtc
     2161 aacatcgtct attcgctata aacattcagt agagacgata gattcagagc acgccgctct
     2221 cattttcagg atgcgccctg tgtggtacag gtcgcaatgc gaaaatgaca ggcatgactg
     2281 gggattctac ggattgattg ccgaggaagt aggagaaatt gcccctcagt ttgtacactg
     2341 gcgaccagct aacgaagatg atgctcctga agctatttcc agcaatggcc ttgttgccga
     2401 aggtgtaatg tacgaacgtc tggttgttcc actgattcac catatccaga aactgactga
     2461 aagagttgat gaacttgagt cagaattgaa gttgttatca acttcccgaa gcgatatcgg
     2521 ataaaggagg agtaatggat ataacacctt ttcttcatgc gctttgtgct gtggctgcgc
     2581 aggtactgat tggccttttt accggaaact gggcttacgg tgcgatagcc ggttgtacgt
     2641 tcttcattgc gcgtgaacac acccaggcag aatatcgctg gattgaaatg ttcgggcatg
     2701 gcaagcgaat gaatatgccg tggtggggcg gttttgatcc gcgcgcatgg gatgtggcaa
     2761 gcctgatgga ttttgctgtg ccggtggtgg cgtgtctgct ggtctggctg ttggttaatc
     2821 gtgggtgaaa aaaggtgagc tgtatatgca acggaggaag aaaactcgtt gctggaagcc
     2881 tggaagaagt atcgggtgtt gctgaaccgt gtggacactt ccgtagcacc agatatcgag
     2941 tggccagctg cacctatagg gtaaaaatat agttctatat gggagcgcta tttttacaat
     3001 ataaatgctg aacggaagaa tctaaccagt tacaaaaaac atagttatgt aatccagttg
     3061 cctgggcatg cgtgtgatgt tgatagctat tactgtatct tccgttcact tatcttattg
     3121 tgaacagtta tatataattt gatctgctct tattcagcct gctaggctgc tgtaaatctt
     3181 cgatactttc ttaacataaa gatcatgatt agaataattg aaagtaagta aacaatccaa
     3241 atggtgatat ttcgttcaat taaaaagcta ccaattccgc cgatcactga aggaatcacc
     3301 agagaagcgg cccccattaa cgcagatgtg gcacccagac tggtacgttg gctggacata
     3361 gttagataag tataaagtga ttcgaacata cctccggcaa acatcaaaac aatgaagaat
     3421 aatataaaga cattgacatg atgaacgaat gttattagcg gagtgcacaa tacgataaat
     3481 gaagcaatga gcttcagcca taaactcaaa atttgtggtg cagaccgctt cttaagtagt
     3541 ttacctgata gtgctgctcc cagcagaagg aatcctccaa taattgatga gataacacca
     3601 aattgcgtag atgagtatcc atacttaacc atcaaaataa atggggaagc tgtgacataa
     3661 caataaatta gagagaatga aagtccaaga gaaaggcaag gtagataaaa tcttggttgc
     3721 ttacttatct ctttgtacgt aagaaatgat gattttagtg aatatgtaat ccgtttttta
     3781 tgactgagtg tttcagggaa gttaaaatag acatacgtta atgttacaac agaatatcct
     3841 aatatcatta gaaaaattgc acgccatccc catacatcat taattattcc tcccagcagt
     3901 ggagaagcaa taggcacgac accttcaata gttatgataa tagcgtacag tgtagcagcc
     3961 actgaaccgc gtgttacatc acgaacagca ctaattgctg taaccagagt tattgatata
     4021 gaaagcccct gtatgatgcg tattgtaaga aacgtaccca atccggtcga gagtgtgagg
     4081 caatatgttg tcacacaata aataatagca cctgaaagca taattcttcg acgaccaata
     4141 gaatcagata atgggccaaa aattagctgt ccaaaaccta tgcaaagggt ataaaccatg
     4201 atagacaatt gcatggacgg gatgttttgt ctaaattctt ttgcgatgtc aggaattgaa
     4261 ggcaaataag tgtccacacc tagaatgccc ataat
//
LOCUS       DARNVM010000258         4288 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.258, whole genome shotgun sequence.
ACCESSION   DARNVM010000258 DARNVM010000000
VERSION     DARNVM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.258"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1192)
                     /locus_tag="SI621_004586"
     CDS             complement(<1..1192)
                     /locus_tag="SI621_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEI4067200.1"
                     /translation="MGKGSNKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHN"
     gene            complement(1253..1885)
                     /locus_tag="SI621_004587"
     CDS             complement(1253..1885)
                     /locus_tag="SI621_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEI4067201.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPSFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGTAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(1822..2565)
                     /locus_tag="SI621_004588"
     CDS             complement(1822..2565)
                     /locus_tag="SI621_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4067202.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLTCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVTLVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            complement(2571..3269)
                     /locus_tag="SI621_004589"
     CDS             complement(2571..3269)
                     /locus_tag="SI621_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEI4067203.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYNGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(3269..3598)
                     /locus_tag="SI621_004590"
     CDS             complement(3269..3598)
                     /locus_tag="SI621_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4067204.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEAAALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFKQVVN"
     gene            complement(3595..>4288)
                     /locus_tag="SI621_004591"
     CDS             complement(3595..>4288)
                     /locus_tag="SI621_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEI4067205.1"
                     /translation="WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGI
                     AQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGASASGGTAIQ
                     AAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGY
                     VGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQ
                     MRDGGLFSGGGR"
BASE COUNT          896 a         1400 c         1072 g          920 t
ORIGIN      
        1 cattatggcg gtccttcagg gcgctgaagc tgtagcggaa cggcgcgcca tcatccggca
       61 tcaccacatt actgcggtta taggtccaca ccttatccga cggtcggtcc tgcacgaacg
      121 tcagcgtctg cccgttccat accggcatac agcgcatcgc cgagcagaaa tcactgagca
      181 catcccacgc cttgcgctgc gtggtcagcc aggcattaca ggtgatgcgc ggctccgtgc
      241 caccaaagcc atccggcacc gactggtcgc aattctggcc gatgacatac agcgcccatt
      301 tgtccacatc cgccgcaccg agacgcttcc ccatgccgta gcgcggatgg gtcagcatat
      361 cccacagaca ccaggccatg ttgttgctgt atgccggttt aaacgtcccg tcccagatac
      421 cgctgtattg ccgcgtctgc gggttatagt tcgacggcac ctgcagaata cgcccgcgca
      481 gatgataatt acggctcacc tgctggctgc cgaactgctc cgagtccacc tgcacgccga
      541 ccagtgccgt gttcgggtag cactgtttca catcgatgat ttcggtgtat gacgaccaga
      601 gcgttttgtt ctgcagctgg tctgtggtgc tgtccggcgt catcctgcgc atccggatat
      661 taaacgggcg cggcggcagg ttatccacca ccactgaggc cagatactgc gaggtggttt
      721 tacccttaat ggtgatgtct ttttccgtca cccagccacc gttacgttgt atctgaacca
      781 gcaggcggac ttccgatgga ttcctgtccc cctttgaggt ggtttccacc agtgcctgca
      841 caccgaaggt aaagcgcaga cggtcgatgt ttgccgacgt gatggtgcgg gtgatcggcg
      901 tgtcatattt cacttccgta cccagcaccg tctcggaacc ggaggattca aacccctccg
      961 gcggagtctg ctcctgctca ccggcccgga acaccaccgt gacaccggcg atgttggtat
     1021 tcccttcact gtccagcacc ggcgtactgt tcagcagcac gctttttaat ccatccaccg
     1081 gaccttctat cggcccttcg ctgatggcat cgatcacact cagtaactgc gtggatttca
     1141 ggttgtcctt cgcttcgcgc ggggtatgcc ccttattgct gcctttaccc attcgtcatg
     1201 ctccataaac gacaaaaccg cccgcaggcg gtttcacata aaacattttg catcagcgac
     1261 caatcaccac aacctgacca ccatcccctt cgtctgccgt gctgatctcc tgagaaacca
     1321 cccgcgaccc cacacgcatt tcaccgtaca gaacaggcaa aacattgccc tgggcaacca
     1381 tgttatccag tgaggagaaa taggtgttct gcttaccgtt atccgttgtc tgtgtgcggg
     1441 gagttctggc tttcggtgcc agcatctgag ccacaccgcc gagcaccata ctggcaccga
     1501 gagaaaacag aatgccggtc ataccaccgg ccccaatggc tgtcccccat gctgcaaggg
     1561 tggctccggc ggtaaagaat gatccggcaa tggcggctgc ccccaggaca atctggaata
     1621 cgccacctga cttggccccg gcgactctgg gaacgatatg aatcacagcg ccgtcaggca
     1681 gagcctcatg taactgcgcc gttaatccgg acgtgctgac atcctgcccg gcaatacgta
     1741 cctgatacca gccgtcgctc agtttctgac gaaacgaagg gagctgtgtg gccagcgccc
     1801 ggatggcttc agcccccgtt ttcacacgaa ggtcgatgcg gcggccaaat cgttgtaaat
     1861 ccccgtaaag gcagatgcgt gccatgcccg gtgacgccag agggagtgtg tgcgtcgctg
     1921 ccatttgtcg gtatacctct ctcgtttgct cagttgttca ggaatatggt gcagcagctc
     1981 gccgtcacca cagtaaatgg cggcatgatt cggcacagat gaaccaaaac agcacagcag
     2041 cacatcgccc ggctgcgccg ctgacagcgg cacctgatac agccctgtgg cctccagatt
     2101 atccagatag agattctgac cgttacgcca ccagtcatcc tcacgatgaa aatccggcat
     2161 ctcaatcccc gccagatggt aagcatcccg gaacagcgtg taacagtccg tcaccccgtg
     2221 ctcaaagagc cgcccggtga gatgcggcac acagcggaac ttatgaatcg ccccccggca
     2281 gaccagccac cacggcaaat cactctgcac ctgcagtcgc cggtcggcct cactcagcca
     2341 gggcagacca ccgggatggc tgtgcaccag tgtcacaatc tcaccctgca tttctgcccg
     2401 cagccagtcc tccggcgaca tccggaaata ctcctccggc tcaccggaga tatttacgca
     2461 ggtaagatat ctgtctccct ccggcgttct caccacaaag ccgcacgact ccgctggcgc
     2521 acatcgccgg gcgtgcgcca gaatcgctga ttctgtctgt gtcatgggat ttactgcgaa
     2581 agtttgttaa tggaaaggaa gccgccaaag ttgccgacgt tattgcgaaa cttacagcca
     2641 ctcaggcatt tgctgcattt atccttcgtg atatcggatg taggctggtc atattcatca
     2701 gcgacagccg gaccgttata accgcactca tcaccgcgat aggtccaggt gcaggtatta
     2761 gccagcatga tgcgccccgg aaaaacggca ccatccgttt ccgtcggtgt ggacagtaca
     2821 aaagaggcac tgaccgcgct cagttcgctg cactgctcga tgcgccagcg gctgatcacc
     2881 tcctgctccg gatcggcgtc gctgtttccg ttgacgaagt tcactgcatc cagaaaacgg
     2941 gcgtaaacct tacgcctgac caccgttccg ccgaccagac tctgcagatc ttccgccatc
     3001 ccggtgacca tgccgtacag gttagaaacc gtcagcgtgg ggcgcgtact ggtgcctttg
     3061 ccattcagtt caaaaccact cccctgaatg ggatacgcct gatactgccg cccctgccag
     3121 gtgaccggct cacctttttc gttctgctca ttacagaaaa aataacgttc tccaccaacc
     3181 tctgtcagat cgatttccca gagcaccacg ctggccgact gctccgcacg ggtgcattca
     3241 ttcagtgttt cctgccggat atcctgcatc agttcaccac ctgtttaaac tctgcgctga
     3301 actcaacacg caacatactg acccgcgacg accattttgc gcaggtcacc tttatctgcc
     3361 ggtagccata aggcggcgtc cacagaaagg ccttccagcc cccgtgctca gccagaaacg
     3421 actccagcgc cgcggcctcc caacggggaa cagaaagcgt cacgctgtac gttttcaggt
     3481 cggcattcag cccggcaggc gctcgctggg aatagccatc accaaagcgc accttcctga
     3541 cggaaggggc cgaagccaca tccataccgg gtttcacttt ccagcggaag gttttcatcg
     3601 tccacctccg gagaacagac caccatcacg catctgcccg gtcacaacat ccattgccgc
     3661 cttacgggct acgtcataaa ccgccttcag cgcctgtggc cctatttgac cgttcgtgcc
     3721 gtcgttgtta atcaccacat ggttattctg ctcaaacgtc ccggacgcct gcgaccggct
     3781 gtccgccatg ctgcccggtg taccgacata accgccggtg gcatagccgc gcatcagccg
     3841 gtaaagattc cccacgccaa tccggctggt tgcctccttc gtgaagacaa attcaccacg
     3901 gtgaacaatc cccgctggct catatttgcc gccggttccc gtaaatcctc cggttgcaaa
     3961 atggaatttc gccgcagcgg cctgaatggc tgtaccgcct gacgctgatg caccgccaat
     4021 ggcgctgcct atacttccga caatccccac cattgcctgc ttaagcagaa tttctgtcat
     4081 catggacagc acggaacggg tgaagctgcg ccagttctgt tcactgccgg tcagcatcgc
     4141 cgccatattc tgtgcaatac catcaaaggt ctgcgtggct gcacttttta cctgcgacat
     4201 actgtccgtg gcgctctctt cccactcact ccagccggac ttcaggcctg ccatccagct
     4261 cccgcgaagc tggtcttcag ccgcccag
//
LOCUS       DARNVM010000259         4277 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.259, whole genome shotgun sequence.
ACCESSION   DARNVM010000259 DARNVM010000000
VERSION     DARNVM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.259"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            64..1722
                     /gene="dpiB"
                     /locus_tag="SI621_004592"
     CDS             64..1722
                     /gene="dpiB"
                     /locus_tag="SI621_004592"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HEI4067206.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1691..2371
                     /gene="dpiA"
                     /locus_tag="SI621_004593"
                     /gene_synonym="citB"
     CDS             1691..2371
                     /gene="dpiA"
                     /locus_tag="SI621_004593"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HEI4067207.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2412..3797)
                     /gene="dcuC"
                     /locus_tag="SI621_004594"
     CDS             complement(2412..3797)
                     /gene="dcuC"
                     /locus_tag="SI621_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HEI4067208.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1182 a          984 c         1079 g         1032 t
ORIGIN      
        1 attttgattc aacattttaa ttacatctgt caaagaggct cgggacaacc cgcaaggaaa
       61 acaatgttgc agcttaacga gaataaacag tttgcatttt tccaaagact ggcatttccg
      121 ctgcgtatct ttttgctgat tctggtgttc tcaatatttg tcattgcagc cctggcgcaa
      181 tattttacgg ccagttttga ggactattta acgcttcatg tgcgcgacat ggcaatgaat
      241 caggcaaaaa ttattgcttc taatgacagt atcatcagcg cagtgaaaac gcgtgactac
      301 aagaggctgg cgaccatcgc tgacaaatta caaagagata ctgatttcga ttatgtggtg
      361 attggcgatc ggcactcgat tcgtctttac catcctaatc cggagaaaat tggttatcct
      421 atgcagttca ccaaaccggg cgcgctggag aaaggggaga gctacttcat caccgggaaa
      481 gggtcaattg gcatggcgat gcgtgccaaa acgccaatct ttgatgacga tggaaaagtc
      541 atcggcgtgg tgtcgattgg ctacctggtg agtaaaatcg atagctggcg tgctgagttt
      601 ttattaccga tggcaggtgt gtttgtcgtg ctgttaggga ttctgatgtt gctatcgtgg
      661 ttcctggccg cgcatatccg tcggcagatg atgggcatgg agccaaagca aatcgcacgc
      721 gtggtccgtc agcaagaggc gctgtttagt tcggtttatg aagggctcat tgcggtggat
      781 ccgcatggtt acattaccgc catcaatcgt aacgcaagaa agatgctggg tctgagttcc
      841 cccggacggc aatggttggg taaacccatt gctgaagtgg tcaggcccgc cgatttcttt
      901 accgaacaga ttgatgaaaa acgtcaggat gtggtggcga actttaacgg tctgagcgtt
      961 attgccaacc gggaagctat tcgttctggt gatgatttgc tgggggccat tatcagcttt
     1021 cgtagtaaag acgaaatatc caccctcaat gcgcaactga cgcaaattaa acaatacgtc
     1081 gagagcctgc gcacattgcg acacgagcat ctcaattgga tgtcgacgct caatggtctg
     1141 ttgcagatga aagagtatga tcgcgtgttg gcgatggtgc agggggagtc tcaggcccag
     1201 caacagctta ttgatagcct gcgcgaggcg tttgccgatc gccaggtggc ggggctgctt
     1261 tttggtaaag tgcagcgcgc ccgcgaactg gggctaaaaa tggtcatcgt ccccggaagt
     1321 cagctttcgc aactgccgcc aggactggac agcaccgagt ttgcagccat tgttggcaat
     1381 ttacttgata acgccttcga agccagcctg cgtagcgatg aaggaaacaa gatcgttgaa
     1441 ttattcctca gcgatgaagg cgatgatgtg gtgattgaag tcgccgatca gggctgcggc
     1501 gttccagagt ctctacgaga caaaatattt gagcagggtg tcagtacgcg tgctgacgag
     1561 cccggcgaac atggcattgg gttgtacttg attgccagct acgtaacgcg ctgcggtggt
     1621 gttatcactc tcgaagataa tgatccctgc ggtaccttat tttcaatcta tattccgaaa
     1681 gtgaaaccta atgacagctc cattaaccct attgatcgtt gaggacgaaa cgccgctggc
     1741 agagatgcat gcggaatata ttcgtcacat tcccggattc agtcagatat tactggcggg
     1801 aaacctggcg caggcccgaa tgatgatcga gcgttttaag ccggggctaa tcttgctcga
     1861 taactatctt cctgacggta gagggattaa tttactgcat gaactggtgc aggcgcatta
     1921 tcccggcgac gtggtgttta ccactgcagc cagtgatatg gaaacggtgt ctgaagccgt
     1981 acgttgtggt gtatttgatt atctcattaa gccaattgcc tatgaacggc tggggcaaac
     2041 gctaacccgt ttccgccagc gtaaacatat gctggaaagt attgatagcg ccagccagaa
     2101 gcaaattgat gagatgttta atgcctatgc tcgcggtgaa cccaaagacg aactgccgac
     2161 cggcattgac cccttgacgc taaacgcggt gcgaaaactg tttaaagagc ctggtgtgca
     2221 acatacggca gaaacggtgg cgcaggcact gactatcagc cgcaccactg ccagacgtta
     2281 tcttgaatat tgcgccagcc gccatctgat tattgctgaa attgttcacg gcaaagttgg
     2341 cagaccgcaa cgcatatacc acagtgggtg acatgataaa gccggaggaa acttgcctcc
     2401 ggcatgacta tttacttgcc tgtgaccgcg gctgccgttc ctggcaccat cagctctgta
     2461 gcaacgataa caatcaccag cccaacaagc accggcaccg aggtgcgttt tacgacttca
     2521 aatggtgaga ttttcgccat cccggcaaca gcaacgacta cgccagaaac gggtgacagg
     2581 gtacgaccga ggtttgacgc ctgcagcatt gggatagtca aatacgccgg gttaatgccg
     2641 gaagagtgcg ccagtttcgg gatcatctca acaaacgcat aaaacggcgc attgcctgaa
     2701 ccggtcgtga ctgccgccag cattgtcagg atcactaata ccagcatcag gatgatactc
     2761 gccgaaccaa acgaggtggc gatagaaatc agactttgaa taaagccgat ggtgctaagc
     2821 ccctgagcga ataccccagc ggcaaccagc agcatcacca cgttagcaaa cgcatctgcc
     2881 atcccgcgat aagccacttc cagaccagag aaaactttct gggtattaaa gctgcggatg
     2941 aactccagaa tggaggcaat cagcatacaa atcaccagaa tagtgatgat gtgtaattgc
     3001 ggaccccatt tgccgtcaaa aatcagcact ccgatgatcg gcgtgaacgg caaaatggca
     3061 taaaacgcag gagcagtggt ggtgatttca ctgacatcta acatttcatg agagatgtgc
     3121 tcttttttat ccagataacg ttgccagaag aagtgggcga tcgccatgcc gataattgca
     3181 gcaattgaga taggcagcgt cgttttgaag gcgaagtcaa tcagcgacat ttcggaagcc
     3241 tgcgccgcca gcaccacatc ccctgaagtc ggtgcgagaa taatcgccgc cggggaggca
     3301 caaatagcag cagctgcacc acggctgata ccaacgttta ccatcaccgg aaacagggtt
     3361 gccatcagca aaacacccag accggtcgcg gaagagacgg ccagagacat cagacaggcg
     3421 acaaaatagg cggcaatcat cagcaggtaa ggggagttaa tatactgcaa tggttttgac
     3481 gccagcttga ccaccatatc attagcgccg atatgggtca tgtaagcggc aaagccacac
     3541 agcatcataa tcatcatgcc gaggtcgccg ccgcggctca ttagcaatat tttaacgtat
     3601 tcaacgatat ccgtggcgct gtagcctgtt gaagcctggc tggacggtaa cactttgtgc
     3661 cccataatgg cactgataat cagcaataac aggccaccga caaataacac gccagtggcg
     3721 gaataccctt taatgatgta gcgagctaca cccacaataa ccacaacccc aataaggagt
     3781 tcaatgaatg tcagcataat ttttcctgtc tccaggcccc aaagtaaata ataaaaaatt
     3841 cctaaagctt aaggaaaaaa atatgcccaa taaattggcg gtgaatgctg attaaaatca
     3901 agaaaaactg ccattaagac attgaagttg ctgtttttat acatagataa caaaacgcat
     3961 atttagcggt tggcaattat cacataataa tgtgtgtaaa ttgatggtgg attgatgcaa
     4021 atttgttaat tgcaatggtg ttagccgcgg gtgtgcgctc aaaaaataat ctaccatgat
     4081 catgggttga cgatatataa tatatttttc gttaagggat ttttgtatct tattaaaaat
     4141 aaagtaattt tttcttggtt gcattttgtc atcaaaaaag acttggtttt tcttttttga
     4201 ctattcccat cgcagaaaac gacgcatcat ctttaatcga tgcgcggaaa tatttaactt
     4261 aaacaagcgg aaataaa
//
LOCUS       DARNVM010000260         4263 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.260, whole genome shotgun sequence.
ACCESSION   DARNVM010000260 DARNVM010000000
VERSION     DARNVM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.260"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            390..998
                     /locus_tag="SI621_004595"
     CDS             390..998
                     /locus_tag="SI621_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEI4067209.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1096..2487
                     /gene="selA"
                     /locus_tag="SI621_004596"
     CDS             1096..2487
                     /gene="selA"
                     /locus_tag="SI621_004596"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEI4067210.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LENEQRFLEMLLK"
     gene            2484..>4263
                     /gene="selB"
                     /locus_tag="SI621_004597"
     CDS             2484..>4263
                     /gene="selB"
                     /locus_tag="SI621_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEI4067211.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLTDAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQVIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRI"
BASE COUNT          953 a         1098 c         1260 g          952 t
ORIGIN      
        1 ccgagcagcg gattgaccgg atggccggac gtcaccccgc cggggcacac cgaacaggcc
       61 acgccggtgg gggcaccgat gcgcaccccg gctgaaccct ggacaatgct accgccatac
      121 tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tctgctcatc cttgttttat
      181 cgggttattt gtctgctgac aggcgaaacc ctacagattt ataagaaagc cttaagtgag
      241 atatctgtca gataagaaca attaggaaaa tatttatatc accatgataa atatgtgatt
      301 tattgggaag taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact
      361 gatatttttc ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt
      421 ttgtacgcaa actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg
      481 aactgcccta taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg
      541 tgctggtgac cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg
      601 aattaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc
      661 gcaaaattga ggcgctggcg gatggcatta tggatgccgg actggtatcg gtgcgtgaac
      721 aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca
      781 accgcagtct ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg
      841 tcaatctggc gactatcgcc attgcctgtg ccgtcggata tctcaatttc cgccgcgttg
      901 cgccaggttg gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc
      961 gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc
     1021 catctcacgt tacaatccgt ggttatgtta aacgcccttc tccgtgtgag agggccttga
     1081 tcagccaggt ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt
     1141 gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc
     1201 gtggtggaat tgctgcgtca gatgcttgac gaagcgcgag aagtgattcg tgacagccag
     1261 acgctgcccg catggtgtga aaactgggcg caagaagtcg atgcccgact gacgaaggag
     1321 gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaatctt
     1381 gggcgagctt tacaggcaga agccgcagtg gaagccgttg cgcaggctat gcgttcgcca
     1441 gtgaccctcg agtacgatct ggacgacgcc ggacgcggac atcgcgatcg ggcgctggcg
     1501 cagctgctgt gccgcattac gggagcggaa gatgcctgta tcgttaataa caatgcggcg
     1561 gcggtgttat tgatgttggc ggccactgcc agcgggaaag aggtggtggt atctcgcggc
     1621 gaactggtgg agattggcgg cgcgtttcgt attcctgatg tcatgcgtca ggcaggctgc
     1681 accctacacg aagtggggac caccaaccgc acgcacgcta atgattatcg tcaggcggtg
     1741 aatgaaaata ccgcgctgtt gatgaaagta cataccagta actacagcat tcagggattc
     1801 accaaagcga tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta
     1861 gtgactgatt taggcagtgg ctcgctggtt gatcttagcc agtacggttt gccgaaagag
     1921 ccaatgccgc aggagttgat tgcggcgggt gtcagtctgg tgagcttctc cggcgacaag
     1981 ttgttaggcg ggccgcaggc aggaattatt gttggtaaaa aagagatgat cgcccgcctg
     2041 caaagccacc cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa
     2101 gccacgttgc gtctttattt acaccctgaa gcactgagtg aaaaattacc gaccctgcgc
     2161 ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggcccccctt
     2221 gccgcacatt acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc
     2281 agtggttcgc tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat
     2341 ggacgcggta gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg
     2401 attggtcgta tttatgacgg acgattgtgg ctggatttac gctgccttga aaatgagcaa
     2461 cggtttttgg agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca
     2521 aaacaacctt attgcaggcg attactggcg taaatgccga ccgtctgccg gaagaaaaaa
     2581 agcgcggcat gaccatcgat ctcggttatg cctactggcc gcagccggat ggtcgcgtgc
     2641 ctggttttat cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg
     2701 gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gctcagactc
     2761 gtgagcatct ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca
     2821 aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc
     2881 tgcgggaata cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg
     2941 gaattgatgc cctgcgcgag catttgcttc agttaccaga acgtgaacac gccagccaac
     3001 atagtttccg cctcgcgatc gaccgtgcat ttaccgtaaa aggtgccggg ctggtcgtca
     3061 ccggtacggc gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa
     3121 ataaaccgat gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg
     3181 ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg
     3241 gcgactggct gcttaccgat gcgccgccgg agccgttcac acgggtgatt gtcgagcttc
     3301 aaacccatac accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg
     3361 tcacgggacg cgtttcgctg ctggaagata accttgccga gctggtattc gacacgccgc
     3421 tgtggctggc ggataacgat cgcttagtgc tgcgcgatat ctcggcccgc aacacgctgg
     3481 cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg taagcgtaag ccggaatatc
     3541 tgcaatggct ggcgtctctt gcacgagcgc agagcgatgc cgatgcgtta tctgttcatc
     3601 tggaacgcgg agcggttaac cttgcagatt tcgcctgggc acggcagctc aacggcgaag
     3661 ggatgcgcga actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg
     3721 cgccggttgc cgcccgctgg cagcggaaaa ttctcgacac attagcgact tatcatgagc
     3781 aacatcgcga tgaacctggt cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg
     3841 aagatgaagc gctggtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca
     3901 gtcatcacgg ttggctgcat ctgccggatc ataaagcggg cttcagcgaa gagcagcagg
     3961 tcatctggca aaaagcagag ccgctgtttg gtgacgaacc gtggtgggtg cgtgacctgg
     4021 caaaagagac gggaaccgac gagcaggcaa tgcgcctgac gctacgccag gcggcgcagc
     4081 aggggataat taccgcgatc gttaaagatc gttattaccg taacgatcgg attgtcgagt
     4141 ttgccaatat gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc
     4201 gcgatcgctt aggtgtaggc cgaaagctgg caattcagat tttggaatat tttgaccgca
     4261 ttg
//
LOCUS       DARNVM010000261         4203 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.261, whole genome shotgun sequence.
ACCESSION   DARNVM010000261 DARNVM010000000
VERSION     DARNVM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.261"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..3397)
                     /locus_tag="SI621_004598"
     CDS             complement(<1..3397)
                     /locus_tag="SI621_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HEI4067212.1"
                     /translation="MNRTSPYYFRRSVLSLLISALIYAQPGMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVYKGGTSNDTTIKGGTSRIEGGTANGTIIDGGGQ
                     SVSTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTAIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVAENVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSEKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMVNGTAEGSIINGGSQVVNEGGLAENSVLNEGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQDAANNILLADGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAVKTTINEGGTL
                     TVNDNGKATDIIQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQALARAEDLLIAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGVVRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSP"
     gene            complement(3462..3581)
                     /locus_tag="SI621_004599"
     CDS             complement(3462..3581)
                     /locus_tag="SI621_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEI4067213.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(3735..3878)
                     /locus_tag="SI621_004600"
     CDS             complement(3735..3878)
                     /locus_tag="SI621_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067214.1"
                     /translation="MWHLVVLLEELCKRGINFRALAQSIFPQQWGDECCKSKTICDLK
                     VIV"
BASE COUNT          981 a         1100 c          895 g         1227 t
ORIGIN      
        1 gagggctgac gccggtatcc tgaacaaaga ttttaaaatt gccagtggcg ttgttgttga
       61 cgaccagttg atcgccccgg gagcctgcaa cgttggtatg caggtagaaa ttaccgctgc
      121 cggaaagttc actggtggtc agcgtattgt agataccgtt agttgtggca ggcgctgctg
      181 tttgtgctga taaataaaca tcaccgtcgt tgaggagtaa actgtttact ctatattcgc
      241 agttgctggt gcctgcacag gaattattgc tgttaagcca gacactgccc cgactggcag
      301 ccgtcaggtc ggcaagcgtc gtatcgacgc catttccgat agttaatgtc gcgctatcgg
      361 taatccggac caccccttca agtttaactg gcgtaacatt atcccgtggc gtcattaacg
      421 acaggcttcc tgtcgcgcca ctgaccgatg catccgccag cgtacctgcg tagacgattg
      481 ccgtcccgcc agcgatcagt agatcttctg cccgggccag agcctgaaac tcatcttttg
      541 accgaccaag ggtatattgc ccaccggaat taacctttgt ggcgaagtcc tgacccaggt
      601 tttgcattgc cccaccgttg ccaaccgtgg aatcgcgagc ttcggtacct gctaatacca
      661 ataaattacc gccattttcc agcagcacat tggtcgctaa atttccggca atggaaaagg
      721 tgccgtactg gtgagtaccg ctgatttcaa taccgttagc cgtgctcgtc tggagagcgg
      781 caccgctgtt ctggatgata tctgtcgctt tgccattatc gttgactgtc agcgtaccgc
      841 cttcattaat cgttgttttt actgcctcac cgttagccga aactgtttgt attccgccgt
      901 cgttaatcgt tgtctcattc gccaccccct cgacgatttg ttcgccgccg gtgagcgtcg
      961 tgcctgtcgc agtggctttt gttttgacga tctcccgccc gcccgcattg acctgtgttt
     1021 tggcggaagt ggtgtctgac tccacggtta acacgccgcc atctgccagc aggatattgt
     1081 tcgccgcatc ctgctcaatg ctgaacgcga cgccatcagc gcgtgtccct gtgacccgcg
     1141 tcgctctggt ggttgcaacc aacgcgccct ggctactttg ctgtatcccc gttgcgctgc
     1201 ctttctcccg cacatcgaga gtgccgcctt cattaagcac cgagttttca gccagaccgc
     1261 cctcattaac tacctgtgaa ccgccattaa taatggaacc ttccgctgtc ccgtttacca
     1321 taatttgttg taggccagag acgatatcgg tattgatcgc cttaccataa ttctgaacgg
     1381 tttgcgtacc gccattgatg tgcgttttgt ctgttgaccc accatcaaca atttgttcac
     1441 cactttcgat atttgcctca gtggctaaac catataccgt ttgcttgcca cctttgatat
     1501 ttgctttttc agaagtggca ctggcatata ttgtttgggt accagcacta ttaagtacag
     1561 taccaacatc ttttccataa acatccattt tgccgttggc attaataatc gtgtcaactg
     1621 cccgggaacc agtgacgact gttaatgagc cagcgttttc cagcactaca tttttagctt
     1681 ctgaattcct gatgtagaaa gcatcaccat aactgttggt tccttcgata agtgttccgg
     1741 aggtcgtgga agcaattaat gcgccgccgg attgttgctc aacatgctta gcctcaccac
     1801 cgtcctgaac ctccagaacg ccaccattat taagtctggt gctatctgtt ttagcctcct
     1861 tctggacaat cagcttaccg ccagcatcaa tggtagtatt tttcgccgag gttttagcca
     1921 ttaccgtcag ttcgccggta ttttccagca caacataatt agcctctcct ccggtaatag
     1981 tgaagtgaga gagcttgttg tatccttcga tatcagtccc tgcgcccgtg ttggcaacta
     2041 aagcactgcc cgtctcctgg ttaaccccat gtgcaatacc gccggtatag acaatcagcg
     2101 agcctccggc gctaatattg ctgccagttg ccgtaccatc tttctcaaca acctgccggc
     2161 tcccagagga tatatttgtc gtgtcagctt tcccgccgct cttgatattt tgcgttccac
     2221 tgttgatatt ggtgcccgtg gcaataccat gattattaat attctgtgtg ccaccattaa
     2281 ttatggtatt tgtcgcgttt ccggcaacat ccataacgcc gccattatct attcgggtcg
     2341 catcagcttt agcattcgtt aaaactgaca ttgttccttt atctttaata atcgtcttat
     2401 ttgccgaacc atatgcgttt atgtctaaat gaccaccgtt ttccagcaac acattctctg
     2461 ccacgttatt gtggatggag aatgcccctt cactattcgt accgctcacc gtcaaatcgt
     2521 aagtcgttac ttttaaagcg gctccatcgt gctgagtaat attagttgcc gtaccacctc
     2581 taacatcaag cacgccgccg gaataaactt caataacatc cgaggagctc gtgttatcaa
     2641 caatttgcgt accaccagaa tagatctgcg tattttttgc cgttgactta ctattcagag
     2701 actgagttcc gccttcaatc gtcgtgtcca gcgcacggct ctcatatact ctttgctcac
     2761 cgccattttt aatggcggtt gtttctactg tgctctgttc aacatactgt cgcccaccat
     2821 ttatggttgt gttcgtcgcc agacttcctt gcgtgatgtc ctgataacca gacttattta
     2881 tcgtcgtacc gtcaacatgc ccctgagttg atactgactg gcctccacca tcaatgattg
     2941 ttccatttgc agtccccccc tctatgcgtg aagtaccgcc cttaattgtc gtgtcattgc
     3001 tggtaccacc tttataaaca tattgattgc cactttcgat tatcgtgcca gtagaaattc
     3061 ctccgtactc aattgactgt ctgcctccat taatggttgt attattagct tgcccgttaa
     3121 tatcggcatg gattgatact agttgctcgc caccagtttc aataatacta ctattagata
     3181 tcccaccata gacctcctgc cggccatggt tgataatatg agtgttattt gttgtacctc
     3241 gttcatctac tcgttggttg ccatctacag tctcatcgtt taccacacca ataacattag
     3301 tagtgaaggc cgccatcccg ggctgggcat atattaaggc agatatcaat aaggaaagta
     3361 ctgagcggcg aaaataatag ggactggtcc tgttcataaa tttcatcctc tgaaaagtga
     3421 atactgagta gcgtttaagc gaccttagct ttgctgcaac atcagcccac aggcaccaga
     3481 ccaggggatt catcctgaag agacagcgca agtgtattgt gttcaccgct catcaaagac
     3541 atcatgatga aatgatgata ttccgcataa gaatgaggca tttttttaag acattgtgct
     3601 gaaggatgat tggataaaaa agtcaatctt ttcaggaaat acggcgtatt cttttctttc
     3661 gacattgaag ccgtcggaaa aataaaatga tttacataat cgtttctgat gaatatcttc
     3721 tgctcacata aaaatcacac aataactttg agatcgcaga ttgttttact tttacagcat
     3781 tcgtcccccc attgttgggg aaatatagat tgggccagag cacgaaagtt aataccacgt
     3841 ttgcacagct cctccaacag cacgacaaga tgccacatac tgcgccccag tcggttcagt
     3901 ttacagacta gcagtgcgta ccctgccgat aatgtcctga gcagtttttc agtccgggtc
     3961 tgctgccacg gttatgcgct gaagtggtaa ctctgagcac gcagttcatc agtcaggcgt
     4021 ggtacaccgt aacgctgtta ttgctgggta atatcaaaaa aactttcagg cagctaagga
     4081 aagttgaacc agacattaga tgaaatattt caaccaatta cagcaccaat tcagtcactg
     4141 ctagcccacc aaataaatca aggggttaca tgaaaacgta gccccttttt ctttggtagt
     4201 gac
//
LOCUS       DARNVM010000262         4141 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.262, whole genome shotgun sequence.
ACCESSION   DARNVM010000262 DARNVM010000000
VERSION     DARNVM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.262"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(49..2025)
                     /gene="speA"
                     /locus_tag="SI621_004601"
     CDS             complement(49..2025)
                     /gene="speA"
                     /locus_tag="SI621_004601"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEI4067215.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2034..2165)
                     /gene="yqgB"
                     /locus_tag="SI621_004602"
     CDS             complement(2034..2165)
                     /gene="yqgB"
                     /locus_tag="SI621_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HEI4067216.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2301..2516
                     /locus_tag="SI621_004603"
     CDS             2301..2516
                     /locus_tag="SI621_004603"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067217.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            2820..3974
                     /gene="metK"
                     /locus_tag="SI621_004604"
     CDS             2820..3974
                     /gene="metK"
                     /locus_tag="SI621_004604"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEI4067218.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1039 a         1132 c         1013 g          957 t
ORIGIN      
        1 cgggccgatt tacgtaaata aatcgccccg ccagcggaat aatcttaatt attcatcttc
       61 aagataggta taaccgtaca aacctgcctc gaactcttca aggaactgtt gttgcagttc
      121 agcatcaaga tcggttttct tcacctgatc gcggaactgg gttaacagcg ttttcggatc
      181 gagctgcaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca gttctacctc
      241 tacgctacca tcagggaaga cgaacacgtc aaccgcttcg gtatcaccga acaggttgtg
      301 catgttgccg aggatctcct gatatgcgcc aaccataaag aaaccgagca tcggcggatt
      361 ctccggatcg tactccggca taggcattgt cgtggcaata ccgtcaccat caatatagtg
      421 gtcgatagca ccgtctgagt cacaggtaat atccagcagc acagcgcggc gttccggcac
      481 ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc acgcatccgg
      541 catcgactgg aacagcgaga agttgacgta cattttgtcc gccatacgtt cctgcagctc
      601 gtcgataatc ggacgatgag cacggttttg cggatccagc tgcttttgca cttcatggca
      661 catgctcaaa taaagctgct cagcccatgc gcgttcttgc aggctaaagg tgccggaaga
      721 gtagccgata tgaatatcgt gcagatccat ctggctgtcg tgtaaccatt cacgcagaga
      781 acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt gcagggcgcg
      841 cggtgcgtct tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca cgccgatgat
      901 attcgacacc agcacggtgt gatgcgcagt caccgcacga cccgattcgg tgattaccgt
      961 tggatgtggc agaccatttt cttcacacgc atcaccaatc gcccagatga tgttgttggc
     1021 gtattcattg aggccgtagt ttacggagca gtcagactgc gaacgggtgc cttcatagtc
     1081 cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct tgtgcagttc
     1141 cacatagaaa cgcgcggatt cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg
     1201 cgaaccgagg tggaagtgca gcagttgcag actgtcgaga cgcccggctt cacgcagcgt
     1261 ttcaaccagt tgcagtacct gagtcgccgc caggccaaac ttcgattttt ccccgccgga
     1321 ggactgccat ttacccgagc cttgcgacgc cagacgtgca cgcacgccca gacgaggaac
     1381 aacattcaga cgttctgctt catccagcac aatggcaatt tctgacatct tctcaatgac
     1441 cagatagacc ttgtggccca tcttctcgcc aattaatgcc aggcggatat attcgcggtc
     1501 cttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca gcactgccat
     1561 caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa tcagggactc
     1621 aatcacgcga cggtgctggt taactttgat cggataaacg aggaagtaat cgccgttata
     1681 gccgtaggat tccctcgctc gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag
     1741 gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcgc gagttttcac
     1801 taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca cgctgatgtg
     1861 gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt aagtacgcag
     1921 catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta gtacaccgtg
     1981 ttcgcctgct gacgaaggca aacccataga catgtcgtca gacatagcga acctcaaatt
     2041 attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga taacaaccca
     2101 taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac cggtttcttt
     2161 ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg aaaccacgag
     2221 aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtagctgta accagctctt
     2281 gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc agagatgcag
     2341 agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag acaacggttc
     2401 attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc aaaactctgt
     2461 tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg
     2521 cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata cgcagaatgc
     2581 cagcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac gattcaaaaa
     2641 aaagtggaaa ttgggtgaag aactgaccta aaatagccat ccagatgtta atccatccat
     2701 accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta ggatccgcta
     2761 ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg atattaaata
     2821 tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac aaaattgctg
     2881 accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg
     2941 cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc accaccagcg
     3001 cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc tatgtacatt
     3061 ccgacatggg ctttgacgct aactcctgtg cggttctgag cgccatcggt aaacagtctc
     3121 ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgca ggtgaccagg
     3181 gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca cctatcactt
     3241 atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc
     3301 tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa atcgttggca
     3361 ttgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa tcgctgcaag
     3421 aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg acttctgcca
     3481 ccaaattctt cattaacccg accggtcgtt tcgttatcgg tggcccgatg ggtgactgcg
     3541 gtctgactgg tcgtaaaatc atcgttgata cctacggcgg catggcgcgt catggtggcg
     3601 gtgcgttctc cggtaaagat ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt
     3661 atgtcgcgaa aaacattgtt gctgctggcc tggccgatcg ttgtgaaatt caggtttcct
     3721 acgcaatcgg cgtggctgaa ccgacttcca tcatggtaga aactttcggt actgagaaag
     3781 tgccttctga acaactgact ctgctggtac gtgaattctt cgacctgcgc ccatacggtc
     3841 tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca tacggtcact
     3901 ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg cgtgatgctg
     3961 ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc tggccttttt
     4021 cttttacctc accgcagtgg acaggcgttc acgccgcatc cggcaaaaaa accgcccgca
     4081 caataacatc attcttcctc aacacgtttt accgcaaatt atcatcacta ctgaaaccga
     4141 t
//
LOCUS       DARNVM010000263         4053 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.263, whole genome shotgun sequence.
ACCESSION   DARNVM010000263 DARNVM010000000
VERSION     DARNVM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.263"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1344
                     /gene="argH"
                     /locus_tag="SI621_004605"
     CDS             <1..1344
                     /gene="argH"
                     /locus_tag="SI621_004605"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEI4067219.1"
                     /translation="MPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQD
                     EANTIIRGLRQVSKEIEDGQFIFDTVDEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQ
                     TTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQTGQPILLSHWIM
                     AYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDS
                     VSDRDHMVEFTAAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNP
                     DVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNTDMSEDKEQVYDSMDTLQASLRIMAP
                     MIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHKKML
                     EELTMEEFSTFDNKIEKDIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK"
     gene            1409..2725
                     /locus_tag="SI621_004606"
     CDS             1409..2725
                     /locus_tag="SI621_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HEI4067220.1"
                     /translation="MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGL
                     KPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVAERILRKKPQAITFVAPILTSVF
                     TLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
                     LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHT
                     EQKKEYVAAPGARKGVAIFAIGVVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAA
                     LLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAYQAELINVFGNLVND
                     APLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQ
                     IGCVAFDRTGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL"
     gene            complement(2841..>4053)
                     /locus_tag="SI621_004607"
     CDS             complement(2841..>4053)
                     /locus_tag="SI621_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HEI4067221.1"
                     /translation="ILTFGFGVPPDSPPITVISIILAVITCIAILQAAGGLDLFVTIA
                     EKILQKRPGAITFLGPAVAYLFTAICGTGYVAFSIYPVIAEIAADARVRPERAMSMSV
                     IAANFGLIASPVSAVVTGTIAVFSGLHVSALDILLITVPGTILGCLVGCLFVYKRGHD
                     LETDPEFQRRVAEGEFESVKAGERTISIISKTAKKALMIFISGIILVVVLGSVPELRP
                     VWNTSAGVERMSIPTALQIIMLTTACIIMMVCRISPSKLDSGSVFKAGLVGVVAIFGL
                     SWMMSSFFEAWQDLFNNTFNDFHNPVIFGVIVFVLSAVIYSPAATAVALFPAGVLMGY
                     STETLIALLPVTCGSFIIPGGAQIACVAFDRTGTTRVGKYVVNHSYMLPGLITVLAST
                     IFCFLFSTILV"
BASE COUNT         1217 a          808 c          898 g         1130 t
ORIGIN      
        1 atgccaacaa ataaactcgt tgaacaatat aatgcaacta tcacgctgga gcagcgccta
       61 tgcccctttg atattcaagg aagcatagtt catgccacga tgttaggacg tcagggaata
      121 atcacacagg atgaagccaa tacaattatt agaggtttga gacaagtaag taaagaaatt
      181 gaagacggcc aattcatttt cgatactgta gatgaagata ttcatatggc cattgaaaga
      241 cggatgactg aaattattgg ccctgttggt ggtaaactcc acacggggcg aagtcgtaat
      301 gatcaaacca ctgttgattc taaaatgcat atgcgagcaa ttatccgtga aattcaagag
      361 gatattacca acctgcaaaa aataataatt aacaaagcag aaaacaatat taatgtcatc
      421 atgccaggct atactcattt gcaaacaggt cagcccattc ttttatctca ctggattatg
      481 gcatattact ggatgttgcg tcgtgactgg aataggtttg aagatctgta tcaacggatg
      541 ggagaatgcc ctttgggggc agcggctctc gccggtacga cattccctat tgatcgtaat
      601 tttacggccc gtgaacttgg ttttgataag ccaactgaga atagtattga ttctgtcagt
      661 gaccgcgacc atatggtcga attcaccgcg gcagcagcga tgtgttttat gcatctaact
      721 cgcctttcag aggaactgat tttattctct agccaagact ttaaatttat tgaactttct
      781 gatgacttct gtacaggatc cagcatcatg ccgcagaaaa agaaccctga tgtggcggaa
      841 aaaatgcgtg gtaaaggtgg gagaatgtat ggaaatctga tggccatgct gactattatg
      901 aaaggcatac cgctagcgta taatacagac atgagcgagg ataaagagca ggtctatgac
      961 tcaatggata ctctacaggc cagcttaaga ataatggcac ctatgatcga aaaaatggtt
     1021 atccttgccg aaaatacgcg tgcagcagcc gctcgaggat tctcgaatgc aacagatatg
     1081 gccgattatc tggtccgtaa aggtattcct ttcagagaag ctcaccatat tgttggtagt
     1141 gcagtaaatt actgtattaa acataaaaaa atgttagaag agcttactat ggaagaattc
     1201 tccacatttg ataataaaat agaaaaagat atttatgaaa gtatttctct ggaggcttgc
     1261 attaaggcca ggatgtctta tggtggaacc ggacctgatg ctgtcaaaaa acaaatagag
     1321 attgcaaaat cacttttaaa atagttaaga attataatgt ttaagattcc ccggatatta
     1381 tatccgggaa tatctttgag gttgaaaaat ggacgctcta ttttttatac aactatttat
     1441 tgttctggcc tgtattggaa tcggcggaaa atacggcgga atggggcttg gtgctggagg
     1501 tgggttaggg gttgcaatat tggttttggg ctttggactg aaaccctcct ctccctccat
     1561 tactgctatg ttaattatta tttgtgttat tagtgctgtc agtatattac aagctgttgg
     1621 aggattagat tatttagtca gggtcgcaga acggatactc cgtaagaaac cacaggcgat
     1681 aacctttgta gcgccaatat taacttcagt atttacacta ttttgtggga cgacttatgt
     1741 cgctttctcc ttataccctg ttattgctga agtcgccgct gaagcaaaag tacgtccaga
     1801 acgtgcatta tctgcaacgg tcattgcagc cagtgtggca gtggctgcca gccctatgag
     1861 tgcagcaact gccgggatgc tggctatact tcatgagtat gcagggatca cgttagggca
     1921 aatattatct attgcccttc cttcgttttt tatggctgca attgttacca gcttttcagt
     1981 ctacaagcgt ggtaaagaac tggaagatga tcctgaattt caaaggcgtg ttgcagcagg
     2041 tgagtatgag ttcatgcaca ccgaacagaa aaaagaatat gtcgctgcac caggtgcaag
     2101 aaaaggggtt gctatttttg ccataggagt cgtgctggtc cttattctcg gatcttttac
     2161 tgagcttctt ccgtcgtggg atggaaaaag attatcaacc ccaatggtca tccagatgat
     2221 tatgttgact gcagctttat tgatcatgat tgtggggaaa gttccaagca ataaatttaa
     2281 cagtggctct gtgttccgtg ctggtttgat gggggttgtg gcaatacttg gtgtttcgtg
     2341 gatgactgca acattttttg atgcttatca ggcagagctg atcaatgttt ttggcaatct
     2401 tgtaaatgat gcgcctttgc tgtttggctt tatcgtattc ttgttttctc tggtgatcat
     2461 gagcccggct gcgacagtag ctgcgattat gccattagga gtcaccctgg gtattcctgc
     2521 accttatctt attgcaattt ttgcctgtac ttgtggtgat tttattatac ccggagcgaa
     2581 tcagattggt tgtgtggcgt ttgacagaac tggtactacc agaattgggc gctttgttat
     2641 caaccatagc tatatacgtc ctgggttcgt catggtcatt tcccaggttg tttttgccta
     2701 cttaattgcg caggttattc tgtaacatta aggaataaat gaagaggata acactgtggc
     2761 atgacgaatt agcccccttc agcgtttttg aggaaggggg attaagaaaa tattacacag
     2821 ctgtaagaat acttctctga ttacactaat atagtggaaa aaaggaagca gaagatagtt
     2881 gaagccagaa cggtaatcag accgggtaac atataactgt gatttactac atacttgcca
     2941 actctggttg ttcctgttct gtcaaacgcc acgcaagcaa tttgtgcacc accaggaata
     3001 atgaatgatc cgcacgttac cggaagcaaa gctatgagcg tctcagtcga atatcccatt
     3061 aatacgccag caggaaataa tgccacagca gttgctgcgg gactgtaaat cacagcagaa
     3121 agaacaaaca caatcacacc gaatataact gggttatgaa aatcgttgaa agtgttatta
     3181 aataaatctt gccatgcttc aaagaaagaa ctcatcatcc atgaaagacc aaatatggca
     3241 acaactccaa ccagaccggc cttaaaaacg gatcctgagt caagtttcga tggagaaatc
     3301 cgacatacca tcataatgat acatgctgta gtcaacatga tgatttgcaa tgctgttgga
     3361 atactcatcc gttctacacc ggcactggta ttccataccg gacgtaattc tggaacagag
     3421 cctaaaacaa caaccaaaat gatcccagaa ataaaaatca tcagggcttt cttcgctgtt
     3481 ttagatataa tactgattgt acgttcccca gcttttactg actcaaattc accttctgca
     3541 actctgcgtt gaaactctgg gtccgtttca agatcatggc cccgtttata aacaaacaaa
     3601 caaccaacaa gacaccccaa aatagttccc ggtacagtaa tcaataaaat atctaaagca
     3661 gaaacatgta atccggaaaa gaccgcaatc gttcctgtaa cgaccgcact tactgggctc
     3721 gctataagcc caaagttggc ggcaatgact gacattgaca tggcgcgttc tggcctcact
     3781 cttgcatcag ctgcgatttc agcaataaca ggataaatgg aaaaagcgac ataaccagtt
     3841 ccacatattg ccgtaaaaag ataagcaact gctggcccaa gaaacgtgat tgcaccaggc
     3901 ctcttttgca gtattttttc agctattgtc acgaaaagat caagacctcc cgctgcctgc
     3961 aggatagcaa tacatgtgat aacagcaaga ataatggaaa tgacagtgat aggcggggaa
     4021 tcaggcggga ctccaaaacc aaaagtcaga att
//
LOCUS       DARNVM010000264         3966 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.264, whole genome shotgun sequence.
ACCESSION   DARNVM010000264 DARNVM010000000
VERSION     DARNVM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.264"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            85..2058
                     /locus_tag="SI621_004608"
     CDS             85..2058
                     /locus_tag="SI621_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEI4067222.1"
                     /translation="MLFAWAINDGPVNVWDITAGGGIPHGLYEAIAAPETLLYFHNSH
                     FDRTVLRYAMPRLAPPVERWRDTMVQALAHGLPGSLGELCEVLGVPQDKAKDKEGKAL
                     IQLFCKPRPKNSKLRRATSKTHPEEWRRFVAYAGLDIEAMREVYKRLPKWNYQGTELA
                     LWHRDQQINDRGVCMDVELARAAIDAVDQEQKRLAKRTQEMTDGEVQAATQRDALIKH
                     IVESYGVELPDMQRSTLERRIADPDLPSAVKELLAIRLQASTTSTSKYKALMKGVSHD
                     GRLRGTLQFCGASRTGRWAGRLFQPQNLPRPSLKQEQIDEGIEALKAGCADLLFDNIM
                     ELTSSALRGCIIAPTGKKLVVSDLSNIEGRMLAWLAGEEWKLNAFREYDAGTGPDLYK
                     LAYAKAFDIAPDDVDKHMRQIGKVMELGLGYGGGVSAFITFALVYGLDLDELANAALP
                     NIPRDVIREAKSWYDESVKRKSTYGLSERVFIACDSLKRLWRRAHPATCDFWYELERT
                     VRTAIATPQKTLYCGYLKIRRDGAWLRIQLPSGRAVCYPSPVIEKGNITYMGVNSYSR
                     KWQRLKTYGGKLVENITQAAARDILAGNMPLIEDAGYSIVLTVHDEVITEAPDTEDFN
                     DKALSALLSTNPEWAPDIPLNAGGFEAYHYRKE"
     gene            complement(2066..3094)
                     /locus_tag="SI621_004609"
     CDS             complement(2066..3094)
                     /locus_tag="SI621_004609"
                     /inference="COORDINATES: protein motif:HMM:NF014567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEI4067223.1"
                     /translation="MSRILFPSSCNPEKIFNFCNEIEKHSGQQQITIDFSHMGRIEPF
                     TMVYVAKHIRDFHRKQKKNIDGFKLFCSGHENKDYAANMAFFRAFGLKHGREPGCTDG
                     NNNFVPFTILRVQTVIDEANDKYTEVQEIVEERSGKLAKILARQEEGDLIDALTFSIR
                     EIMRNVVEHSDSKVIEYCAQYWPSYDRVEITISDNGIGMKKSLSKNPYIEADNDSEAI
                     QLALMPSISSKNFKGARINTRNPWHNSGFGLYMISRICKLGGSFLICSGDHAIYLDEH
                     GKKHIELGHYHEGTVVRMVLNTKKLGSLSSMLTQFKEDGYKIAAEIKHTGVYTASAAS
                     QMLSRDFK"
     gene            3155..3442
                     /locus_tag="SI621_004610"
     CDS             3155..3442
                     /locus_tag="SI621_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEI4067224.1"
                     /translation="MSYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRLVFVECKAPGKAPRADQLREHERLRELGFTVVVLDSKNLEEIILSRNKR"
     gene            complement(3455..3652)
                     /locus_tag="SI621_004611"
     CDS             complement(3455..3652)
                     /locus_tag="SI621_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067225.1"
                     /translation="MVDGSNTKVLLNSFGSGFVVGDCESNSAKFEFVNDLKGMKFTVM
                     SSKKEIAKLKECFETRVKNTD"
BASE COUNT         1120 a          964 c          869 g         1013 t
ORIGIN      
        1 aatatactat ggggcgacct ggaaacctat tgcgaaatcc ctattacgaa cggtacccat
       61 gcgtatgccg aaggcgttga agtgatgctt ttcgcatggg ctatcaacga cggaccagta
      121 aacgtgtggg atatcactgc cggtggtggt attccacacg gcttatacga agcaatcgca
      181 gcccctgaaa ccctacttta ttttcataac tctcacttcg accgcaccgt cctgcgttat
      241 gcaatgccgc gcctagcacc gccagtcgaa cgttggcgcg acacaatggt gcaggcgctg
      301 gcgcacggtc tcccggggtc tctgggggaa ctctgcgaag tactaggcgt cccgcaagac
      361 aaagcgaagg acaaagaagg taaagcgctg atccagctgt tctgtaagcc acgcccgaaa
      421 aacagcaaac tgcgccgggc caccagcaaa acccacccgg aagaatggcg gcgctttgtt
      481 gcttacgccg gactggatat cgaggcaatg cgcgaagtct ataaacgtct gccgaagtgg
      541 aattatcagg ggacagaact ggcgctctgg catcgtgatc agcagatcaa tgaccggggc
      601 gtctgcatgg acgtggaact cgcacgcgct gcgatcgacg cggtagacca ggaacaaaag
      661 cgcctggcaa agcgtacaca ggaaatgact gatggcgaag tgcaggcagc cacacaacga
      721 gacgcgttga ttaagcacat tgttgaatcc tacggtgtgg agctaccaga catgcaacgc
      781 agtactctgg aacgtcgtat tgccgacccc gatttaccat ctgccgtgaa agaactgctg
      841 gctatccgcc tgcaggccag tactaccagc accagtaaat acaaggcact gatgaaaggc
      901 gtaagccacg acgggcgctt acgcggtacg ctacagttct gcggggcgtc acgtaccggt
      961 cgttgggccg gacggctatt ccagccccag aaccttcccc gcccttcact aaaacaggaa
     1021 caaatagacg aaggcatcga agcactgaaa gccggatgtg cagacctgct gtttgacaat
     1081 atcatggaac taaccagttc agcgttacgt ggctgcatta tcgcgccaac aggcaaaaag
     1141 ctggtggtaa gtgacttgtc gaacattgaa ggccgtatgc tggcatggct ggcgggagaa
     1201 gaatggaaac tgaatgcatt cagagagtac gacgccggaa cgggtccgga cttatataaa
     1261 ctggcgtatg caaaagcttt cgatattgca ccagatgatg ttgataaaca catgcgtcag
     1321 atcggtaaag tcatggaact cggtctgggt tatggtggtg gtgtgtcggc cttcatcact
     1381 tttgcactgg tttacggtct cgatctcgac gagctggcga acgccgcact accaaacatt
     1441 ccccgcgatg ttatccgcga ggcgaaaagc tggtacgacg aatcggttaa acgcaagtca
     1501 acctacgggc tttctgaacg ggtatttatc gcctgcgact cacttaaacg tctctggcgc
     1561 cgggcgcatc ccgcgacctg tgatttctgg tacgaactgg agcgcaccgt ccgcactgca
     1621 atcgccacac cgcaaaaaac attgtattgc ggttatctta aaatccgccg cgatggcgcg
     1681 tggctgcgca tacagctacc atccggacgc gctgtatgct acccgtctcc ggttatcgaa
     1741 aaagggaata tcacctacat gggggttaac tcttattcgc gtaaatggca acgactcaaa
     1801 acctacggcg gaaagctggt ggagaacatc acccaggcgg ccgcccgcga cattctggcc
     1861 ggaaacatgc cgcttatcga agatgccggt tacagcattg tgctgacggt acacgacgag
     1921 gtgatcaccg aagcacctga cacagaagat ttcaacgata aagcgctttc cgcgcttctc
     1981 tccactaacc ccgaatgggc acccgatatt ccgctgaatg ctggcggctt tgaggcgtac
     2041 cactaccgta aggagtaata caaagtcact taaaatccct agataacatc tgcgaagcag
     2101 ctgatgctgt ataaactcca gtatgtttta tttctgcagc tattttgtag ccatcctcct
     2161 taaactgcgt aagcattgag cttaaggaac caagtttttt agtattcaac accattctaa
     2221 cgacagttcc ttcatgataa tgacctaact ctatatgctt ctttccgtgt tcatctagat
     2281 atattgcatg gtcaccgcta caaataagaa aactaccgcc aagtttacat atacggctga
     2341 tcatataaag gccaaatccc gaattatgcc aaggatttct tgtattgatt ctcgccccct
     2401 taaaattttt actagaaata gatggcatta acgctaactg aatggcttca gaatcattat
     2461 cagcttctat ataagggttc ttcgatagag attttttcat tcctatacca ttatcagaaa
     2521 ttgttatttc aacacggtca taagaaggcc aatactgtgc acaatattca atcacctttg
     2581 aatcactatg ttccacaaca ttacgcataa tttccctaat agaaaatgtc aaagcatcaa
     2641 tgaggtcgcc ttcttcttgg cgagcaagaa tctttgctaa tttaccagat cgttcctcaa
     2701 ctatctcttg cacctcagta tatttatcgt tagcttcatc tataactgtt tgaacacgaa
     2761 gaattgtaaa tggaacaaaa ttattatttc catctgtaca tcccggttca cggccatgct
     2821 ttaacccgaa ggctcgaaag aatgccatat ttgcggcata atccttattc tcatgcccgc
     2881 tacaaaaaag tttaaaacca tcaatgttct ttttctgttt tcgatggaag tctcgaatat
     2941 gcttagcaac ataaaccatt gtaaatggtt caatccttcc catatgcgaa aaatcgattg
     3001 ttatttgctg ttgacctgaa tgtttttcaa tttcattgca aaaattaaat atcttttcag
     3061 gattacatga agagggaaat aagattcgtg acatattaat tccttatact tgttttattt
     3121 gatactaccc aataagtgtt aacaaggcaa atttatgtct tatgaacgtg agagccttat
     3181 cgaaaagcac ctcgtcgctg aagtgaaaaa ggctggcggt gtggccttta agttcgtatc
     3241 tcccggtcgc cgctcggtac cggatcgcat tgttctgcta cccggcggcc gtcttgtttt
     3301 cgttgaatgc aaagcacccg gcaaagcacc acgcgccgac cagttgcgcg agcacgagcg
     3361 gctgcgcgag ctgggcttta ccgtggtggt gctggatagc aaaaatctgg aggaaattat
     3421 tttaagccgc aataaaagat aaaagactta tgctttaatc cgtattttta acacgtgttt
     3481 cgaaacactc ttttaattta gctatttcct ttttagagga cataactgtg aatttcatcc
     3541 cttttaaatc attaacaaat tcaaattttg cgctattgga ctcacaatca ccaacaacaa
     3601 aaccactacc aaatgaattg agcaacactt tagtattact accatctacc atataatatt
     3661 cgttattttt aaatgcagta accttcccga aggtatagct caatatcaat aatgtcatca
     3721 cccctaaagg aaaagctgat tttaggattt ttaaatcgat ataataaaat acgtctttat
     3781 actctgcaag cctcccaaac tttctaatgc tatccatatg atgttcgtgt ctcttactat
     3841 ggattgacgc ggacaccgca ttgtggtaat tcaacaaaag cagagcaaat aataaaatat
     3901 ctccaacact catcacagag gtttcggaga aataaccaaa aggccaatga aatcctatta
     3961 tgtaaa
//
LOCUS       DARNVM010000265         3561 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.265, whole genome shotgun sequence.
ACCESSION   DARNVM010000265 DARNVM010000000
VERSION     DARNVM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.265"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(195..2012)
                     /gene="malZ"
                     /locus_tag="SI621_004612"
     CDS             complement(195..2012)
                     /gene="malZ"
                     /locus_tag="SI621_004612"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HEI4067226.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVAMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRILVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2168..3541)
                     /gene="proY"
                     /locus_tag="SI621_004613"
     CDS             complement(2168..3541)
                     /gene="proY"
                     /locus_tag="SI621_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HEI4067227.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
BASE COUNT          914 a         1029 c          859 g          759 t
ORIGIN      
        1 ttgtcatatc tttgctccac tacagggtta tcctggagca tgttgtacat aacatcatgg
       61 gcaaagaata ccaaaaatcg ctcatatcga tcgggacaaa tatttaacaa attcagccct
      121 gacattgagc agcttttttg cgctgcgtag tgtcagggct ggtgactaac gacgtaacaa
      181 ataaaacaga tgctttagtt catccacacc gtagccgaaa tggcaggcaa agccagaatc
      241 ccgtctgtca gttgcccatg gccttctttg cgttgccatt gcacgacatt aagaaacggt
      301 gacgcgggta gcaccacttc acaggcctcg ccacggttga ttgccaccag tatacgctgc
      361 tgattcagca cgcggacaaa taccaccacg ttatcttccg catacagcac ctgacagccg
      421 ccacgacgca gcgcctgact tttcttacgc agcgcaatca ttcgctggta caacgcgaat
      481 aacgccgtat cctgcttttc cacctgccag gggaacggct tacggcaaaa cggatcgttt
      541 ttaccatcca gtcccacttc atcaccgtaa taaatgcacg gtacaccagg ccaggtgaac
      601 aaccagacca ccgccagcgg caggcgcgca atatcccgac cgagcagcgt tttaaatcgc
      661 gcagtatcgt ggctatcgag ctgattaaac atacgcagtt gctgttgatg agaaagtccg
      721 gcgcggtaat tatccatcca ggccatacag gtttgggcgt caatttgctg cggatcataa
      781 gagatgtcgg tattggcaag aaatccccac aacgggaatg tgaagccgcg atagttcatg
      841 gcggcatctt ccacatcggc ctgtaaccat tgccgtgcat cgccaaaatg ctctccaact
      901 atatacgctt ccggctgggt ttctttcgcc gcttcggtga tcccggctac gtgctgcaaa
      961 ttattgcgcg ccccacccgc ttcccccagc atatgcacca catccagccg ccagccgtcc
     1021 atattccacg gcgctttcag ccagtggcgg acaatactgt cttccccgcg ataaatttca
     1081 ttcaccagac tttccgactg ataatccagc ttcggcaagc tggcatagcc cagccagtcg
     1141 agcgccgtgc catcgtcact aaacgagtac cagtcgcgcc agggcgattc ggggttgtga
     1201 caagcaccac ccgtcccgcg attgtgtctg tcaaaccagg catgggaatc gccactgtgg
     1261 ttaaacacgc cgtccagcac cagccgcatt cccagctgct gcgtattgtg tcgcaaacgc
     1321 aacaacgccc catcaccgcc aaactgcgga tcgacatggc gataatcctc ggtatcgtat
     1381 ttatgtacgc tgggagcttt aaacaccgga ttgagataca gcgccgtcac gccgagcttt
     1441 ttcagatacg gcagtttttc gctgataccg tccagatcgc cgccatagaa cgttgatccg
     1501 cccgcctgcg ccgtgaccgg ttcatcccag tcacgcaata tgatctcttg tccggctgcg
     1561 tgatggtaat aaacatgatc ctgttcagct tcacgaggaa gactacgggc gaaacgatca
     1621 gggaagatct gataaaaaat ctgatccgca gcccattgtg ggccgatatc cgggacatcg
     1681 acggcaaact gctccagtcg tgctggcggc attcggctga agccctgcgg tgtaaaccaa
     1741 cgctggcgat cgtgccacag caatttgaaa ctataacgcc gccggggttg cccgctggag
     1801 agatcgatcg ccgcccgcca tgcggtaacg ccaggctgcg gctgactgcg ctgcttatgc
     1861 atcgctactg acgtttcttc gttgtcgtgt tctgtacgca gcataatgcg ctgcggtggg
     1921 tcttcgcccg tcagccacag cgtaatgagc agttgatctt tgctttgttt aacaaatggg
     1981 ggcaccggca ggtgccatgc atttaacatc ataattcccc tgtgatgaaa atctgatcac
     2041 cttgccatag cctaatgcag gatcactcat catctgaagg atatttgggg gaggaggacg
     2101 gaggcggata actttcggaa tagccggatc aggatttcct cctgaaccgg cttacagaac
     2161 gcaaggatta gtggttttca gccagctgac gatcatggcg gcgcttaaac atccagccaa
     2221 tcaacagcac aacaatccac gcgaaaccga catacagcga gatacgcgta tcagggtgat
     2281 aaccaatcaa cccgataata aagagcagga aaatcaaacc gccgatggtc gttgctaccc
     2341 cacccggtac tttaaatttc agcgccttaa cttcttctgg cggtaaacgg cgacggaagg
     2401 cgatttgcga cagcaggatc ataatccaca cccacaccgt ggcgaaggtc gccagcgaag
     2461 cgatcaccag gaagacgttt tccggcatga tgtagttcag atataccgca aacaatagcg
     2521 cggtagtcat caccagcacc gtgacccacg gaataccacg acgcgacgtt ttgctgaaaa
     2581 tttttggcgc gctgccctgc tctgccatac catggagcat acggcctacg ccaaatacgt
     2641 cactgttaat tgccgaaagc gaagcggtca gcacaacaaa gttaaggatg ctggcggcaa
     2701 aggtaatccc catatgctgg aacgtcagta cgaacgggct accggcagtg ccaacctgat
     2761 tccacgggta gatagacata atgacgaaca gcgtaccgac gtagaacacc agaatacgca
     2821 tcggcacgga gttaatcgcg cgcggaatag atttctcagg atctttcgct tcaccggcgg
     2881 taatcccgat aatttcgatc ccaccgtaag caaacatcac catttgcaac gacatcacca
     2941 taccaagcca gccgttactg aagaagccgc cgttgctcca caggttatga ataccggtcg
     3001 gttgcccgcc gttgccaatc ccccagatga tgatgccgaa accggcgaca atcatgatga
     3061 tgatggtggc gactttaaag aacgagaacc agaactccag ctcaccgaat accttcacgc
     3121 tcatcaggtt tacggcgcaa atgatcagca ccacgctcag tacccaaatc cagtgcggca
     3181 ccgtcgggaa ccagacaccc atatagatac caaaagcggt cacatcggcg atggcgacaa
     3241 taaggatttc aaagcagtag gtccagccag taatgtaacc agccagcggg ccgaggtttt
     3301 cctgcgcata acgcgagaaa gagctggcgg ccgggttatg taccgacatt tcccccagcg
     3361 cgcgcataat gatatacgcc gcgacaccac cgataatata ggccaacaac acgctcggac
     3421 cggccatttt gatggcgtct gccgaaccgt aaaacaaccc ggtgccaatt gctgaaccca
     3481 gtgccataaa gcgtatgtgg cgggtactta gcccacgctt tagcttgttc ttactttcca
     3541 tcaattccta acccatcaac a
//
LOCUS       DARNVM010000266         3511 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.266, whole genome shotgun sequence.
ACCESSION   DARNVM010000266 DARNVM010000000
VERSION     DARNVM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.266"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            142..1536
                     /locus_tag="SI621_004614"
     CDS             142..1536
                     /locus_tag="SI621_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEI4067228.1"
                     /translation="MNNIFHPRLYQNLIINHQTDILRGNIWAGMGMGKTVATLTTLED
                     LFMAGAETHPALVLAPLRVAASTWPDEAVKWGHLRNIEVQPIIGNAKARSTALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVR
                     RWINLTGTPAPNGLVDLWGQSWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(1730..3127)
                     /locus_tag="SI621_004615"
     CDS             complement(1730..3127)
                     /locus_tag="SI621_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEI4067229.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKSTFGE
                     REVFSDVTYSRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            3342..>3511
                     /locus_tag="SI621_004616"
     CDS             3342..>3511
                     /locus_tag="SI621_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEI4067230.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIP"
BASE COUNT          913 a          882 c          876 g          840 t
ORIGIN      
        1 tatgccatcg cataagctaa cgctgaaaaa acagttaata aagaaacttg ttttgtaagt
       61 atttttgcca ggggcatacc cgcctccgtt agttatatat atagagagag aattgacaca
      121 tattctacca taggtgtatt aatgaataac atattccacc cccgacttta tcaaaacctc
      181 ataatcaatc accaaaccga cattctgcgc ggcaacatct gggcagggat ggggatgggt
      241 aaaaccgtag cgacactcac cacgctggaa gatcttttca tggctggagc agaaacacac
      301 cccgcgctgg tcctcgcgcc gctacgcgtt gcagcaagca cctggcctga tgaagctgtt
      361 aaatgggggc atctgcgtaa tattgaggta cagccgatca tcggtaacgc caaagcgcgc
      421 tctacggcgc tggcgaacag caacgcgagc gtgttcacca tcaactacga taaccttgtc
      481 tggctggttg aagaattggg agaacgatgg ccgttcggaa ctgtcattcc agatgaaagc
      541 acccggctaa aatccttccg gctgcgaggt ggtggtaagc gcgcggcggc gctgggcaaa
      601 gtggcgcata agtatgtccg gcgctggata aatctcaccg gtacgccagc accgaacggc
      661 ctggtagatt tgtggggaca atcgtggttt gtggaccaag ggcaacgtct cgggcgcact
      721 tacggcgcgt ttacctcacg ctggttcaac tcgatacagt ttccgggaca gagctggact
      781 aaactggaac cttttgctca ctcacagggt gaaatacagc gagcattagc cgatgtgacc
      841 ctctcgctgg atgcggccga ctggttcgat atcaaagacc ccatccataa cgtaatccgc
      901 gtggatatgc cgccgaaggc ccgtcagcag tatcgtgaaa tggaaaagga aatgttcctc
      961 gagctgaatg gcgaaggcat cgaagcaccg aacgccgcgg caaagacact gaagtgtctg
     1021 caaatcgcca gcggcgcagt atacacagat gacaccggaa gttggtcaga actgcatgac
     1081 accaaactac aggcgctgga cagcatactg accgaagcag ctggcgcacc tgtgctggtt
     1141 gcttatcact ggaaacacga tcttgaacgc ttgcttaaag catttcctcg cggtcgtcac
     1201 ctcgaccagg atccacagac actacgtgac tggaattccg gaaagattcc tgttctcttt
     1261 gcacacccag ccagcgcggg ccacggtctg aacatgcagg acggcggaaa catactggta
     1321 tttttctcac attggtggga cctggagcag tatcagcaaa ttattgaacg tatcggccca
     1381 accagacaga tacaggccgg acacaatcgt ccggtattta ttcaccacat tattgctgcc
     1441 gacactatgg acgaaatggt gatggagcgg cgcaactcaa aacgaacagt gcaggacatc
     1501 ctgctcgatg ccatgaaaaa gagaggtata gcatgagcga gaaacccgac gatttactca
     1561 ccccggatga agtatgccaa aagttaggta ttacacagaa aacgctatgt gagtggaata
     1621 ttaagcatcg tcatcgggct atcctggcac caattcgttt cagtgcaaaa gtagttcgtt
     1681 atgagcgccg aaatgtcgac gcttttattc aaaaatgtcg cagccagtat taacctcgcc
     1741 gccgtagcaa tgccacctgc gcaagtatgc tccgctcgtg agcctcgaaa gcttcgcgct
     1801 tcaacgcaat ctcttcctgt aaaatctcat cagaaaagtc gtaatgttct gccatcgggt
     1861 catctgactt gctggagtgg tgaaggcaaa ggaggctgat ttcccttcgg tctgagcggg
     1921 aatagcctct ttccttcatc aaggcaataa cattgctctt aaggaattta cggcacatcg
     1981 tattaaatgc accgtctttc cctttaacag tcccatcatg ttttattcct tttacagccc
     2041 cgtccgggct gtatgttttc accagcttat ccagtgatcg ttttgaaaat ggctgcattg
     2101 gatcacgtgg ctgcaaaaat acataatccc tgttgcactc aggaactgaa tcacgccagg
     2161 ctttctgctc gtcgataatc cgccggatct caggcgttat cggcaggcgg aaagcctttt
     2221 gtgttttcat agcccctcgc atgccgataa ccccttccgg ataaacaatt tcaccagcct
     2281 cctcgtgaac gtagtcccag cgcaggttat ggacattaat cggacgaaca ccagtgatga
     2341 tcatgaagcg aacagcattc ttctggtgta cagaggtgca ggcagcaaca ttgagccaga
     2401 gtcgggcgat tgattcaata tcggtaaaaa gccgtgtggg ggtaggtttc tgtacgcggg
     2461 aggaaacata atcatctggc agactggcgg caacattgcg gccgttgcaa agagtaggtg
     2521 cgcagaactt ccagaaccga cggagctcgg caaacaactc cagggcgtta ttgttcgagc
     2581 gagtggcgat ccactcgtcc agcacttcca ccagccgact gtatgttacg tcgctgaaca
     2641 cctcgcgctc gccgaacgtt gatttaatcc ggtcgatacg caccccgtag gttgtgaaac
     2701 tgtccgggct cagcttctga cgggctactt tggctttgag gtcatcccgg tacatttcca
     2761 gcgctgcatg tacggactct gcccgcagtc caccgtcagc catacctagt gctttttcgc
     2821 gcgccagctg gatagcgagc tccggccact cgccgagctt tttacctttg aggcccatct
     2881 tttttggaaa ctcggcgtaa aatgtaacct taccggcttt gctgaaatcg atacggagat
     2941 agttctcttt ttcgtatttg gaacggcgag ccacgccgga agcagcgagg atgattttgg
     3001 cggcagcaac acagattttc atgtgtgcgc tagtataggg gggtttacag gcgtcccatt
     3061 tttcagaagc ggctaaaaca tcgtcattat tggggctatc cggattatgt gttacagtgc
     3121 gcggcattct caatccttat ctgcgtaggc gcagaaaaca agctcacaca tacaagtctt
     3181 ttctacggga caaaatgcaa tgtgttgcgg ttttgtgttg ctggactgag tttatcaagg
     3241 ttaaatacac tggatcaaca tacagtaagt taatgacagt aaagcataca aactcgatac
     3301 aacttactga ttttaaaatg atttaacggt aattcattga aatgtcttta ctaattacta
     3361 aacgctgtat taattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
     3421 gcgatcatat ctatgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
     3481 caccaacctg ccagaaggtg tgcccgatcc c
//
LOCUS       DARNVM010000267         3475 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.267, whole genome shotgun sequence.
ACCESSION   DARNVM010000267 DARNVM010000000
VERSION     DARNVM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.267"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            39..800
                     /locus_tag="SI621_004617"
     CDS             39..800
                     /locus_tag="SI621_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HEI4067231.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEHPVPEEQPEEIATATVTKISECDLTATIEQRGPLTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAALVTKNDGKAFPQ
                     PAGAALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHIANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            816..1238
                     /locus_tag="SI621_004618"
     CDS             816..1238
                     /locus_tag="SI621_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEI4067232.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFTSEQARKDWNNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDGRLNIICSECRKSEVMKRVLMFYRGSIQEFIR"
     gene            complement(1375..1647)
                     /locus_tag="SI621_004619"
     CDS             complement(1375..1647)
                     /locus_tag="SI621_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067233.1"
                     /translation="MKIDIKNKNGNTQHLDVSSLIITLNNGETIEITDENKSRPIDIP
                     EGVTVWGGRAPDKEASIDQLKKTTRSIGIYPLASNMVHIFPYSLKK"
     gene            1918..2586
                     /locus_tag="SI621_004620"
     CDS             1918..2586
                     /locus_tag="SI621_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013096685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEI4067234.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKNENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGR
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDSGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EGQE"
     gene            complement(3199..>3475)
                     /locus_tag="SI621_004621"
     CDS             complement(3199..>3475)
                     /locus_tag="SI621_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HEI4067235.1"
                     /translation="ENVPSALLTHHNTAAGVFGQLCVMEGTVTYYGFADENTTEPEIK
                     VVINAGSFATSPPQYWHRVELSDDAQFNINFWVAPDFSGEKVYSTKK"
BASE COUNT          974 a          698 c          828 g          975 t
ORIGIN      
        1 aggaattgcg aagtgatttt taccgggagg aaattttaat ggaaaccgtt tttgatgcac
       61 tgaaagcaat gggaaaagcc acgtcggtag aactggctgc gcgacttgat atcagtcgtg
      121 aagaagtgct gaacgagctg tgggaactga aaaaggctgg attcgttgat aaaagcgtat
      181 acacctggcg tgtggccgat aacaacgttc agcaggaaca tccagtgccg gaagaacagc
      241 cggaagaaat cgccacagca acagtgacga aaatatcgga gtgcgattta accgcgacga
      301 ttgaacaacg tggtccacta actgcggatg aactggctac gttgttcggt accacatcac
      361 gcaaagtggc ttcaacgctg gcaatggcaa tcagcaaggg gcgtctgatt cgcgtaaatc
      421 agaacggtaa atttcgttac tgcatgccgg gcgataattt accagcagaa ccgaaagctg
      481 cattggtaac gaaaaatgat ggtaaagcct ttcctcagcc agccggtgct gcgttaccag
      541 tacaggaagc ggcaacacag gaagatatta aaacagaaac tgtggcggac attgtgcagt
      601 cgctgccatc gtttactgaa acgcaagcgg atgacttggt tttaccatcg ctgcatatag
      661 caaaccgcga actgcgtcgg gcgaaaaatc atgtccagaa gtgggagcgt gtctgcgccg
      721 cgctgcggga gctgaacaag caccgggata ttgttcgaca gattgtcgat tcctccagtc
      781 gtattgtgtc ggaaaagtga ttgccggagg cgcttatggc aaaagttttt acacaggaag
      841 agcgtgaaaa aattaaggag caggttgttg agctggtacg ccagagtggg cgcgagacgt
      901 taaggcaact ggaggccaag acaggggcga caagatatct gatgagcatt cttgccagag
      961 agttggttgc cagtggcgat gtatacaact ctggctatgg gttatttacc tctgaacagg
     1021 ctcgtaagga ctggaataac gcccgcaaaa aactatccag ggcaaaggtg aaaaaaacgg
     1081 ttgtggttga tcctgacctt atctggtcat taccagacgg agaaatacgc cgatatgacg
     1141 ggcgtctgaa tataatctgt agcgagtgcc gtaagagcga agttatgaag cgtgtactga
     1201 tgttttatcg aggaagtatc caggaattta ttcggtaagc aaaatcaaaa tgtaatcgct
     1261 caagtttaag ttggtgtatc tacggcgcag agccatcgcc tcatctggca ctgcgctaaa
     1321 atcaaattta gttccactct ttatttcgta tctgctaaaa cgctacataa aattttactt
     1381 ttttaaagaa taagggaata tgtgcaccat attggaggct agtggatata ttcctatgct
     1441 acgagttgtc ttttttagtt gatctatgct tgcttcttta tcgggagccc ggcctcccca
     1501 cactgtaact ccctcaggta tatcgattgg gcgacttttg ttttcatctg tgatttcaat
     1561 cgtttcccca ttattaagcg taataatcaa cgatgacacg tccaggtgtt gtgtattccc
     1621 atttttattt ttaatgtcta ttttcatggc tatttcatta tatgttatct gtgctggagt
     1681 aataagttta cagtattaag aagcattttg acgaaacaat caactgactt atcagctaag
     1741 gtggcattgt aatgcgatct gattggtatc atcaggacgc tgttcaaaaa agggggatta
     1801 gttgatcttg aaatcttgct caaaatgtgt agcctctcgt ttaactgcca actttttggg
     1861 gcagtacact ctgatgctac acaacttgca agtttcatta ccccgacgta aacatttatg
     1921 acagttgctg atttcaaacg tcctaagttg gagctcccaa acggggcaaa caaactacta
     1981 ttacactctt gttgtgcacc atgttccggt gaagtgatgg aggctcttca ggcctcagga
     2041 atcgactaca ccatcttctt ctataatccg aacattcatc ctcagaaaga gtatttgatt
     2101 cgtaagaatg agaatattcg atttgctgaa caacatggtg taccgtttat cgatgctgat
     2161 tacgacactg acaactggtt tgagcgagcc aaaggaatgg aatgggagcc cgaacgtggg
     2221 aggcgttgta ctatgtgttt tgacatgcgt tttgaacgga cggcgctgta cgccgctgaa
     2281 aatggtttca gtgtgatcag cagttcactg ggcatttcac gctggaaaaa tatgcagcag
     2341 gttaacgaca gcggacggcg agctgtcgca cattatccgg gcatggtgta ctgggattat
     2401 aactggcgca agcagggagg ctcgtcccgc atgattgaaa tcagcaaacg cgaacaattc
     2461 tatcagcagg aatattgtgg ttgcgtgtat tcacttcgcg ataccaattt acaccgcaaa
     2521 tcccagggac gccctcttat caaaattggt caactccact acggtaaaga agaagggcag
     2581 gaatgatttt tacattacgt caacagagtg tcacaacgtt tctatatatg gtatgccgac
     2641 cgtaattttc gaatcatcat gactaatagg caggtcacct atgccagcaa tgacctgctc
     2701 atcttgaaat tagcagaaat cttgtagcta tgatgtagct caaaatagtt tttcattctt
     2761 ttacctgatg tctgttcctg gctgaaaaca gactgtatta tttgattacg caccactgac
     2821 acttgtgtat tgaagtagtg agtacaaagc cacatattta tcagtgtatg tatacgtcaa
     2881 aaacaggaga ttgttgtgct ggttctgaag tgtgcgttag caatagtggc tgtggtggca
     2941 atttattgcc ttgttgttgt tcttatggag cgactttctg attgattaca tactggctaa
     3001 aaaaaagcgt ttgcctggct tatctttgtc cggcaaggtg cgctcattaa tctgaagtaa
     3061 atttatttct taagtattca gattacttct tatggtcgaa gtctcctggc aatgtagaac
     3121 caaaatattt acaaaaacga gacccgtcga ggatagctct gccgggtctg attgttaaag
     3181 tatttttaat atgatcatct attttttggt gctatagact ttttcgcctg agaagtctgg
     3241 agcgacccaa aagttaatat taaactgagc atcatcgctt agttcaacac ggtgccagta
     3301 ttgtggtggg cttgttgcaa aagagccagc attaataact acttttatct ctggttcagt
     3361 agtattctca tcagcaaaac cataatatgt tacagtgcct tccattacgc acaactgccc
     3421 aaaaacacct gctgctgtat tatgatgtgt taataacgct gaaggtacgt tctct
//
LOCUS       DARNVM010000268         3424 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.268, whole genome shotgun sequence.
ACCESSION   DARNVM010000268 DARNVM010000000
VERSION     DARNVM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.268"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            88..1608
                     /locus_tag="SI621_004622"
     CDS             88..1608
                     /locus_tag="SI621_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEI4067236.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARKRQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1634..1972)
                     /gene="maoP"
                     /locus_tag="SI621_004623"
     CDS             complement(1634..1972)
                     /gene="maoP"
                     /locus_tag="SI621_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HEI4067237.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2091..2930
                     /gene="hdfR"
                     /locus_tag="SI621_004624"
     CDS             2091..2930
                     /gene="hdfR"
                     /locus_tag="SI621_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HEI4067238.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYKNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3025..3100)
                     /locus_tag="SI621_004625"
     tRNA            complement(3025..3100)
                     /locus_tag="SI621_004625"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3065..3067),aa:Trp,seq:cca)
     gene            complement(3109..3185)
                     /locus_tag="SI621_004626"
     tRNA            complement(3109..3185)
                     /locus_tag="SI621_004626"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3149..3151),aa:Asp,seq:gtc)
     gene            complement(3240..3355)
                     /gene="rrf"
                     /locus_tag="SI621_004627"
     rRNA            complement(3240..3355)
                     /gene="rrf"
                     /locus_tag="SI621_004627"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          900 a          855 c          848 g          821 t
ORIGIN      
        1 taaaccggaa tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg
       61 cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc
      121 ctgggagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctacccggc
      181 ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag cccgcgatcg cgtgcgcagc
      241 gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa tctggctcca
      301 gccgatctgc caaaagaagg gggacgatat gatttaccta tcgccattgc gttgctggcg
      361 gcctcagaac agcttacagc caataaatta gatgaatatg aattagtcgg agaactggcg
      421 cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agctattaag
      481 tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt
      541 gaaggatgcc tgatagccga tcatctgcag gctgtctgtg cgtttctgga gggtaagcac
      601 gctctcgaac gcccgaaacc aactgatgca gtatcccggg cgctacaaca tgatctcagt
      661 gatgttgtcg gtcaggagca aggaaagcga ggactggaaa ttaccgccgc tggcgggcac
      721 aaccttttac tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat
      781 ggcctgttgc cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg
      841 gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac
      901 agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg
      961 ctggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg
     1021 gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa
     1081 ataacctatc cagcccgttt ccaacttgtt gcggcgatga atcccagccc taccggacat
     1141 tatcagggaa accataaccg ctgcacacca gagcaaacgc tgcgttatct caaccgactc
     1201 tctggtccct ttctcgaccg cttcgatctc tcactggaga tcccattacc gccacccggc
     1261 attttgagta aaacggtagt gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg
     1321 gccgccagaa agcgccaatt taagcggcag aataaactga acgcctggct ggataatccg
     1381 gaaatacgcc aattctgcaa gcttgagagc gaagatgcgc agtggttgga agaaacgctg
     1441 atccatctgg ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt
     1501 gctgatattg atcagtccga cattatcaca cgtcagcatt tgcaggaggc agttagctat
     1561 cgtgccattg accgtttgct catccatctg caaaaactac tgacataaaa aaaagggcat
     1621 ttcgcccttt ttattaatcg tcagaatcgg tgtagtcttc agcaccttca acctgcggtt
     1681 taccgccgga aagggtgtga aaacgttttg gacgcttgat acgcgtcata tacttggacc
     1741 acacgcgttc tgcttctgtc actggctcac gttcgccacg gcatactgct acgaagagtt
     1801 tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa ctcattgaag gcataaccat
     1861 gacgctcaag cagttgtgcc tctttgatgg tgaagtcacc atgacgagag aatccacgtg
     1921 gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg
     1981 ctcctaattc tttgaccgag ctagttatgg cgcggagtat tagttacgct tgacagagtg
     2041 taaaacaaaa catttaaatc ataacgacaa ataattttgc ggagagcact gtggatacgg
     2101 aattgttaaa aactttcctg gaagttagcc gaacgcgtca ctttggtcga gcggctgaat
     2161 cgctctatct gacccaatca gccgtgagct ttcgaatcag acaactggaa aatcaactgg
     2221 gtgtgaatct tttcacccgc cacagaaaca atatccgttt aaccgccgcc ggagaaaagc
     2281 ttctgcctta tgcagaaacg ctcatgagca cctggcaggc cgcccgtaag gaggtggcgc
     2341 atacctcacg acataacgag ttttctatcg gtgccagcgc ctcattgtgg gaatgtatgc
     2401 ttaatcagtg gctgggacgt ttgtataaaa atcaggatgc ccatacaggc ttacagttcg
     2461 aagcgcgaat tgcccaacgg cagtctctgg taaaacagct gcatgaacgc cagcttgatc
     2521 ttcttattac tactgaagcg cccaaaatgg acgaatttag cagtcagttg ctcgggtatt
     2581 tcactttagc gctttatacc agtgcccctt ccaaactaaa gggagatctt aactatctgc
     2641 gactggaatg ggggccagat tttcaacagc atgaggcagg tttgatcggt gctgacgaag
     2701 ttcccattct gacaaccagt tctgctgaac tggcacagca acagattgcg acgcttaatg
     2761 gttgcacctg gctacccgtc agttgggcgc gtaaaaaggg cggcctgcat accgttgtcg
     2821 atagcacaac actttcacgg ccgctttatg ccatatggct gcaaaatagc gataaaaatg
     2881 cgttgattcg cgatctattg aaaattaacg tgctggatga agtgtattaa tctgaatggc
     2941 tggcaaggat gccggtagaa ggatttactt cggagaggtt atttcagata aaaaaaatcc
     3001 ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg
     3061 gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga
     3121 cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
     3181 gctccaccga attcttttac aaccaccggt tttatgaccg gcttactgct taatttgatg
     3241 cctggcagtt ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt
     3301 tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg ccaggcaaat
     3361 tctgttttat taacccgctt accgccggtt aatttaatct gtatcaggct gaaaatcttt
     3421 ctct
//
LOCUS       DARNVM010000269         3329 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.270, whole genome shotgun sequence.
ACCESSION   DARNVM010000269 DARNVM010000000
VERSION     DARNVM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.270"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            81..196
                     /gene="rrf"
                     /locus_tag="SI621_004628"
     rRNA            81..196
                     /gene="rrf"
                     /locus_tag="SI621_004628"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(297..824)
                     /gene="mobB"
                     /locus_tag="SI621_004629"
     CDS             complement(297..824)
                     /gene="mobB"
                     /locus_tag="SI621_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEI4067239.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVSLNLDVALLDINDVG
                     GLADFVVEWMQKQNG"
     gene            complement(806..1390)
                     /gene="mobA"
                     /locus_tag="SI621_004630"
     CDS             complement(806..1390)
                     /gene="mobA"
                     /locus_tag="SI621_004630"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEI4067240.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQETGEWFLFCPCDT
                     PYIPPDLAARLNHQRKEAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1460..1729
                     /locus_tag="SI621_004631"
     CDS             1460..1729
                     /locus_tag="SI621_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HEI4067241.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1806..2792
                     /gene="srkA"
                     /locus_tag="SI621_004632"
     CDS             1806..2792
                     /gene="srkA"
                     /locus_tag="SI621_004632"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HEI4067242.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2809..>3329
                     /gene="dsbA"
                     /locus_tag="SI621_004633"
     CDS             2809..>3329
                     /gene="dsbA"
                     /locus_tag="SI621_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HEI4067243.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFV"
BASE COUNT          831 a          812 c          825 g          861 t
ORIGIN      
        1 tgttttggcg gatgagaaaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa acagaatttg cctggcggcc ttagcgcggt ggtcccacct gaccccatgc
      121 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
      181 tagggaactg ccaggcatca aattaagcga aaggccattc gaaaggatgg cctttttgcg
      241 ttggtgaaaa aaatgccgga tgcgacgctg gcgcgtctta tccagcctac tcttgcttat
      301 ccgttctgct tttgcatcca ctccaccaca aaatcagcca gccccccaac atcattaata
      361 tccagtaacg cgacatcaag attaagtgac acatcactgg ctacagcaat aacatgtctg
      421 tctatcacta attcttccgg tcgatgtccg gctccatcgc gaaacagcac aatctttgcg
      481 atctcttcat gcttaaaccc ttcgaccaga atcaaatcca gctttgaggt atccattcga
      541 cttgcgagaa aatgtagatc cagctcttct tcgtctggtg tttccgtcat caaggcccat
      601 cgctgctggc tggcaacgat ggtttgtgcc gcgccagcct ttcgcagctc atagctatct
      661 ttgcccggtt tatcaacatc catatcatgg tgcgtatgct taatcagccc tggacggatc
      721 cctctggcgc ataatgccgg gatcagtttt ttcaaaagcg tagtttttcc agtgccgctc
      781 cacgcggcaa aggcgagtaa cggtatcatc gtttttcctg ccatcgggca agctcctctg
      841 gcgtattcac gttaataaat gcatctttat tatcgctgaa atcaaccgca tgaccgccag
      901 ccagacgcat aaatgccatt actcggcgtt ctcctgcttg cagatattcc agtaataaag
      961 gctcaattgc gcggtttacc agagcaatcg tcgggtgatc gcgttcaccg tcgtggaccc
     1021 acacgacagg tgcctctttg cgctgatggt taagccgggc tgctaaatca gggggaatgt
     1081 aaggcgtatc gcacggacaa aacaaaaacc actcccccgt ttcctgctgc attactgaaa
     1141 gcattcctgc cagagggcct gggtaatccg ccagtgaatc ttcaatcact ttcagaccgc
     1201 ttgcctgata gatttcctga tgacgattgg cattaaccac gacgtgcgag agctgcgtca
     1261 taagcgcgtc agcgacatgt tgccacagcg gtttgccgtt taattcaagc aatcctttat
     1321 ctacgccgcc cattcgtctg gctttaccgc ccgccagcac aacgcctgtt atcgtcgtca
     1381 tcagattcac cgatatcgcc tcttttattg tgggattgac cctgctaacg tgtctgtctc
     1441 aagagtaagg agcattacta tgaaatgtaa acgtctgaat gaagttattg aactcctcca
     1501 gccagcctgg caaaaagagc cagaccttaa cctgctgcaa tttttgcaga aactggcgaa
     1561 agagtcaggt tttgacggcg aactggcgga tttgacggat gacattctga tctatcatct
     1621 gaaaatgcgt gattccgcga aagatgcggt tatcccgggt ttgcaaaaag attatgaaga
     1681 agatttcaaa acggcgctgt tacgcgctcg cggcgtaatt aaagagtaaa agcttgtaag
     1741 cggcgccacc aaaatcatcg tgaaatgata tccttcatca ttcgtaatgt tttccggatg
     1801 atgggatgaa caacagcgct tttactttcc agacactaca cccggatacc atcatggatg
     1861 ctctatttga gcaagggatc cgggtggatt ccggtcttac cccgcttaac agctatgaaa
     1921 accgtgtcta tcaatttcag gacgaagatc gtcgacgttt tgtcgtcaaa ttttatcgcc
     1981 ctgaacgttg gacagccgac caaatcctcg aagaacatca gtttgcgttg cagctggtaa
     2041 atgatgaagt tccggtcgca gcacctgtgg cctttaacgg tcagacttta ttgaatcatc
     2101 agggatttta tttcgctgtt tttccaagcg tcggtggtcg ccagttcgaa gctgataata
     2161 tcgatcagat ggaagcggtt gggcgttatt tagggcgtat gcaccagacg gggcgcaaac
     2221 agctttttat ccatcgcccg accatcggtc tgaatgaata tctcattgag cctcgcaagc
     2281 tgtttgagga cgctacactg ataccttccg ggttgaaagc ggcatttctg aaagcgacag
     2341 atgagctgat cgccgccgtt acagcacact ggcgggaaga tttcaccgtt ctgcggctac
     2401 atggagactg ccacgctggg aatattctct ggcgcgatgg tccaatgttt gttgatctgg
     2461 atgatgcacg taatggtcca gccgttcagg atttgtggat gttgctcaat ggcgataaag
     2521 ccgagcagcg gatgcaactg gaaactatta ttgaagctta tgaagaattt agcgagttcg
     2581 acaccgctga aatcggactg attgaacctt tacgcgccat gcgtttggtt tattatcttg
     2641 cctggctaat gcggcgttgg gctgatcccg cgttcccgaa aaatttcccg tggttaaccg
     2701 gggaagatta ctggctgcga cagacggcga cttttataga acaggcaaaa gttctacaag
     2761 aacccccttt gcaattaaca cctatgtatt aatcggagag agtagatcat gaaaaagatt
     2821 tggctggcgc tggctggttt agttttagcg tttagcgcat cggcggcgca gtatgaagat
     2881 ggtaaacagt acactaccct ggaaaaaccg gtagctggcg cgccgcaagt gctggagttt
     2941 ttctctttct tctgcccgca ctgctatcag tttgaagaag ttctgcatat ttctgataac
     3001 gtgaagaaaa aactgccgga aggcgtgaag atgactaaat accacgtcaa cttcatgggt
     3061 ggtgatctgg gcaaagatct gactcaggca tgggcagtgg cgatggcact gggcgtggaa
     3121 gacaaagtca ctgttccgct gtttgaaggc gtacagaaaa cccagactat tcgttcagca
     3181 tctgatatcc gcgatgtatt tatcaacgca ggtattaaag gtgaagagta cgacgcggca
     3241 tggaacagct tcgtggtgaa atccctggtc gctcagcagg aaaaagctgc agctgacgtg
     3301 caattgcgtg gcgttccggc gatgtttgt
//
LOCUS       DARNVM010000270         3252 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.271, whole genome shotgun sequence.
ACCESSION   DARNVM010000270 DARNVM010000000
VERSION     DARNVM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.271"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            92..1774
                     /locus_tag="SI621_004634"
     CDS             92..1774
                     /locus_tag="SI621_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEI4067244.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKESFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1826..2983
                     /locus_tag="SI621_004635"
     CDS             1826..2983
                     /locus_tag="SI621_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEI4067245.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIA
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2949..3071)
                     /locus_tag="SI621_004636"
     CDS             complement(2949..3071)
                     /locus_tag="SI621_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067246.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          997 a          692 c          650 g          913 t
ORIGIN      
        1 tatctcttta tgtgactaac ttcacttaca ttcacttatt tctcttcgta aaattacttt
       61 ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt
      121 aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg ctcatgcggc
      181 agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact ttacgccgac
      241 tgaatacagt accaaaggaa agccaaatat tatcgtactg accatggatg atcttggtta
      301 tggacaactt ccttttgata aggaatcttt tgacccaaaa acaatggaaa atcgtgaagt
      361 tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcgcaaa aatcaacgcc
      421 gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg tggcgcacgg
      481 tgtttccggc ccctcccgcg ccgcaataat gaccggtcga gcgcccgccc gctttggtgt
      541 ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct tgcctgaatt
      601 attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt caaaaatcag
      661 taatgtgcca gtaccggaag ataaacaaac gcgtgactat catgacaact tcaccacatt
      721 ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg gattccacgc
      781 tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac gcgtccccgc
      841 aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa
      901 aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc tgccaaatga
      961 taatcctgca ccggagcaat atcagaagca atttaatacc ggtagtcaaa cagcagataa
     1021 ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa
     1081 gaaaaacgga cagtatgaca atactattat tctctttacc tccgataatg gtgcggttat
     1141 cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga cttatcctgg
     1201 cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaaccag gtaattatga
     1261 caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg atatcagcat
     1321 tccaaaagac cttaagctgg atggcgtttc cttgctgccg tggttgcaag ataagaaaca
     1381 aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt ttgacgagga
     1441 aaatattcca ttctgggata attaccacaa atttgtccgc catcagtcag acgattaccc
     1501 gcataacccc aataccgagg acttaagcca attctcttat acggtgagaa ataacgatta
     1561 ttcgcttgtc tatacagtag aaaacaatca gttaggtcta tacaaactga cggatctaca
     1621 gcaaaaagat aaccttgccg ccgccaatcc acaggtcgtt aaagagatgc aaggcgtggt
     1681 aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg agaagtttaa
     1741 caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg cggatttttc
     1801 cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca gttttcaatg
     1861 taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa
     1921 atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa
     1981 tcaggtctat tttacgtggc aaggcggtga acccactctg gctggtctgg attttttccg
     2041 taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta atgcattaca
     2101 aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac atgagtttct
     2161 ggttggtatc tcgatcgatg gaccccagga gctacatgac cgttacagac gcagtaattc
     2221 tggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat catatcaagt
     2281 agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc ttgaggttta
     2341 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg
     2401 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
     2461 tgtgcctcct acggcttatg gcaagtttat gtcgaccatt tttatgcaat gggttaaaaa
     2521 cgatgtgggt gaaattttca tccgtcagtt tgaaagtttt gtcagccgtt ttttggggaa
     2581 tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
     2641 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
     2701 taaatctgaa cttaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
     2761 agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgcc ctaagcatcg
     2821 tattgctaaa gtaaacaatg agactgtttc ctatttttgc gaaggttata aaatcctttt
     2881 ttcaaccatg gtaccttata tgaacgccat ggtggagtta gccaagaaca gagtaccgct
     2941 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct ccttctttgc
     3001 tccagtctta gcctccgtct tatgacctta acaaagcacc cgacataaat tcctgttggg
     3061 tgtttcgtca tcatcatctt cccttgcgaa aattctggct tacgtggcat gcgtcagggc
     3121 attagcaata cccttgatta tcaatagcat aatcgtaacc attgaaaata aaaaatttct
     3181 ttatatttca aaatgttaaa tgacacttaa ataaatttca tcagtaaatc attgccatag
     3241 ataatcattg ga
//
LOCUS       DARNVM010000271         3223 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.272, whole genome shotgun sequence.
ACCESSION   DARNVM010000271 DARNVM010000000
VERSION     DARNVM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.272"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..2793
                     /locus_tag="SI621_004637"
     CDS             <1..2793
                     /locus_tag="SI621_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEI4067247.1"
                     /translation="ISAVTASINNSSLSRNVTFIADVSTAKIADLVVIKDGSEADGST
                     ANTLQVKVTDANGNTLAGQTVSVLAGNSATVTPTVTTKPDGTVEISVTSQTAGISAVT
                     ASINSSSQSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLRARVTDAFGNALAGQ
                     TVSVLAGNSATVAPTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQSQNVTFVPGDA
                     SQLTSIVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAADGVLTVAGTDPSEMG
                     SWVESGGVYTTTRMATIASTNQHANLQLQTWSDGVTSDRYDIQSGSPAQATSTIATDK
                     NAYTAGDTITVAVTLKDAHGNLVEGGESLLSGDNVIVEGAVRSGGWSENAGVYTATWS
                     AQMAGDSHHATLKLSEWGSSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTIT
                     LRDEFGNPALGLTSEVIESYIDSFAVGGATPDSMRWVEQNNGEYTIVWTAWVADENLV
                     ASLKLKTWATEIKSSLYGIQPGAAAKTQSTIVADKTIYIAGDSITVTVVLKDAQGNFI
                     TDGVVQLNEENVQVRNADPIQGNNWVYNGNGQYQRQYMAHFAEANLNAQLKMAGWSDA
                     NYSNNYTIKPGEVSPLGSQLRIREVLVVEGADLPVSALLVDDFGNPVDNGLDLLDDAV
                     YLQNVEKKEGEKWRYVGDGIYERTYMAYQEGENLTSFMEIKGWRIYGQPSYTILPFVE
                     VELLSVNGVKFRATDGFPETGFDGAKFTLLLTHNMKNTDYNWTAGIYGINVDSNGEVT
                     LSVLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGATSSNTWDIINTSCSYGQMPS
                     SLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSGTDYWTSTQLMGVHEKFNPETGI
                     SELGTGKSSGLCVEYY"
     gene            complement(2845..>3223)
                     /locus_tag="SI621_004638"
     CDS             complement(2845..>3223)
                     /locus_tag="SI621_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEI4067248.1"
                     /translation="IITSILAMFMALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKF
                     LIYGASIKNDIMKLLANINTNKINGVILPLIYTVYGKKEYEKRWQQLIKDNDLWSNVL
                     LHDPRLLSVKNEFNTIGVMRTSA"
BASE COUNT          932 a          653 c          850 g          788 t
ORIGIN      
        1 atcagtgcgg tcacagccag catcaacaac agcagtctga gccggaacgt gacatttatc
       61 gccgatgtca gcacggcgaa gatcgctgat ctggtggtca ttaaggatgg ttcagaggca
      121 gacggttcga cggcgaatac gctgcaggtg aaggtcaccg atgctaacgg taatacgctt
      181 gccggacaga cggtcagcgt gttggcaggc aacagcgcaa cggtcactcc gacggtgacc
      241 accaagccgg acggcacggt ggagatatct gtaaccagcc agacggcggg aatcagtgca
      301 gtcactgcca gcatcaacag cagcagtcag agccggaacg tgacgtttat cgccgatgtc
      361 aggacagcga agatcgcgga cctggtggtc attaaggacg actcagtggc agacggtgca
      421 atggcgaata tgctgcgggc gagagtcacc gatgcgttcg gtaatgcgct tgccgggcag
      481 acggtcagcg tgttggcggg caacagcgca acggttgctc cgacgatgac cacaaagccg
      541 gatggcacgg tggagatcag tgtcaccagc cagacggcgg gcaccagtac agtcaccgcc
      601 agcatcaaca acagcagcca gagccagaac gtcacctttg tacctggtga tgcatcgcaa
      661 ctcacttcaa tcgttgaaac aaataagtcg aactatacgg ttggagagac aatcaccatc
      721 acggtaacgc tccgggatgc gttcgataat ctggtaaccg gtgcagcttc acagttagcc
      781 gctgatggtg tgctgacggt ggctggcact gacccgtcag aaatgggaag ctgggttgaa
      841 tcaggtggcg tgtataccac aacccgaatg gcgacgattg ccagtaccaa tcagcacgcc
      901 aacctgcaat tgcagacgtg gagtgatggc gtaacgtcag atcgctatga catccagtcc
      961 ggttctccgg cgcaagccac atcaactatc gctacggata aaaacgccta caccgctggc
     1021 gacactataa ccgtcgccgt gacgctgaaa gacgcgcacg gtaacctggt tgaagggggc
     1081 gagtccttac tgtctggtga taacgtaata gtggaaggag ccgtacgttc aggaggatgg
     1141 tctgaaaacg ccggtgttta tactgctaca tggtcggcgc aaatggcggg agactctcac
     1201 cacgcgacgc tgaagttgtc tgaatggggc agcagtaaac aatcagagag ttattccatc
     1261 cacagcggtg ctccggtgca ggcaaattct gctatcagga cagataaatc ggcatatatc
     1321 gccggggagc cattaaccgt cacaataacg ttgcgggatg agtttggtaa ccctgcttta
     1381 gggctgacat cagaagtcat cgagtcttat attgatagtt tcgcggtagg cggcgcaact
     1441 cccgattcta tgcggtgggt cgagcagaat aatggtgaat ataccattgt ctggacagca
     1501 tgggttgccg acgaaaatct ggttgccagt ctgaaattaa aaacatgggc aacggagatt
     1561 aagtcctcgc tatacgggat acaaccaggt gcagcggcaa aaactcaatc aacgattgtc
     1621 gcggacaaaa cgatatatat cgctggggat agcataacgg tgaccgttgt tcttaaagat
     1681 gcccagggta actttattac tgatggtgtt gttcagctta atgaggaaaa cgtacaagtc
     1741 aggaatgcgg atcctatcca gggcaataat tgggtttaca acggcaatgg gcaatatcaa
     1801 agacagtata tggcgcattt tgcggaagcg aatttgaatg cgcaactgaa gatggctggt
     1861 tggagcgatg ccaattattc aaacaattac actattaaac caggtgaagt gagtccgctt
     1921 ggttcacagc ttcgtatacg agaagtttta gtagtggaag gggcggattt acctgtcagt
     1981 gctttattag ttgatgattt tggaaatccg gtggataacg gtctggattt gctggatgat
     2041 gcagtgtact tacaaaatgt agaaaaaaaa gaaggggaaa aatggagata tgtgggtgat
     2101 ggaatatatg aacgtacata tatggcctac caagaaggag aaaatttaac ttcatttatg
     2161 gagattaaag gttggcgtat atacggacaa ccatcctata ccatccttcc ttttgtggaa
     2221 gtcgagttac tgagtgttaa tggtgtaaaa ttcagggcga cagatggttt tccagaaaca
     2281 gggtttgatg gtgcaaaatt cacgctgtta ctaacccata atatgaaaaa tacggattat
     2341 aattggacgg ctgggattta tggaattaat gttgacagta atggagaagt aacattatct
     2401 gtgctgataa gaagtgaagt tactattact gggaaaccca agaatggtaa agggaatgat
     2461 gttgtattta aattcaagat taaaaaatgg tttactagtt taggtgctac cagtagtaat
     2521 acctgggata tcataaatac ttcatgtagc tatggtcaaa tgccgtcatc tttagaatta
     2581 gcgcagcggc caagcggtgg ggtagtaccg cgaaaagtag gtacgttgtg gggcgaatat
     2641 ggtaatttaa aaacttatgg gaacgctttt agcggtacgg attattggac aagcactcag
     2701 ctcatgggag tacacgagaa atttaatcct gaaactggta tatcagagct tgggacagga
     2761 aagtcttctg gcttgtgcgt agagtattat taacgaagta attactaaat tgccctgcat
     2821 cagcagggca atttatattc gttattacgc agaagtacgc atcacaccga tagtattaaa
     2881 ttcatttttt acactgagca aacgggggtc atgaagcaaa acatttgacc ataaatcatt
     2941 gtctttaatt aattgttgcc agcgcttttc atattctttt ttaccataca cagtgtaaat
     3001 caacggcaaa atcacaccat tgattttgtt ggtgttaata tttgccagta acttcataat
     3061 gtcatttttt atagatgcgc catagatcag aaatttcgta taaagcgagt taacacaggt
     3121 gtaatcaaga ccatgttcag gctcaagttt aagcaataaa cttttggctt tgtcattatg
     3181 tcctttcagt gccatgaaca tagccagaat agaggtaata att
//
LOCUS       DARNVM010000272         3191 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.273, whole genome shotgun sequence.
ACCESSION   DARNVM010000272 DARNVM010000000
VERSION     DARNVM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.273"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..2145
                     /locus_tag="SI621_004639"
     CDS             <1..2145
                     /locus_tag="SI621_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEI4067249.1"
                     /translation="EDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQT
                     VDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLDREITLPSSGTT
                     LISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIR
                     ENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEY
                     QVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLALGNYSLTVR
                     AVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEK
                     RIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSAFVEAVGRASD
                     DAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDANDKWNAMWAVKI
                     EQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFM
                     NDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGNVNANSGTLNNVTINENCR
                     VLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAF
                     SGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSV
                     SAWLVNDWYPTASISDLLVVVMKKATAGITIS"
     gene            2204..>3191
                     /locus_tag="SI621_004640"
     CDS             2204..>3191
                     /locus_tag="SI621_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HEI4067250.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEAMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAASSQT
                     ASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQVAA
                     AQSESAAAGSATSAAGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKTSETAAKN
                     SQDAAAQSESAAAGSASAAAASATASANSQTAAKTSETNAKTSETAAANSAKA"
BASE COUNT          832 a          815 c          959 g          585 t
ORIGIN      
        1 gaagatacgc aggccattct ccgttacggt cgtaacgtca cgaagatgga tgcctttggc
       61 tgtaccagcc gggggcaggc acaccgcgcc gggctgtggc tgattaaaac ggagctgctg
      121 gaaacgcaga ccgtggactt cagcgtgggt gcagaagggc ttcgccatgt accgggcgat
      181 gtcattgaaa tctgtgatga tgactatgcg ggtatcagca tcggcgggcg cgtgctggcg
      241 gtgaacagcc agacgcggac actgacgctc gaccgtgaaa tcacgctgcc atcctccggc
      301 accacgctga taagcctggt tgacggacag gggaatccgg tcagcgtgga ggtccagtcc
      361 gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc agaatacagc
      421 gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt gagtatccgt
      481 gagaacgatg acggcacgta tgccatcact gccgtgcagc atgtgccgga gaaagaagcc
      541 atcgtggata acggggcgca ctttgacggc gaccagagcg gcacggtgaa tggtgtcacg
      601 ccgccagcag tgcaacacct gaccgcagaa gtcaccgcag acagcgggga ataccaggtg
      661 ctggcgcgct gggatacgcc gaaggtggtg aagggcgtga gctttatgct tcgcctgacc
      721 gtggcagcgg atgacggcag tgagcgactg gtcagcacag cccggacgac ggaaaccaca
      781 taccgcttca ggcagctggc tctgggaaat tacagtctga cagtccgggc ggtaaatgcg
      841 tgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgccgcacc ggcagcgcca
      901 tcgcggattg agctgacgcc gggctatttt cagataaccg ccacgccgca tcttgcggtt
      961 tatgatccga ctgtacagtt tgagttctgg ttctcggaaa agcggattgc ggatatcagg
     1021 caggttgaaa ccacagcccg ctatcttggc acggcgctgt actggatagc cgccagtatc
     1081 aatatcaaac cgggccatga ttattatttt tacgttcgca gtgtgaacac cgtcggcaaa
     1141 tcggcatttg tggaggccgt cggtcgggcg agcgatgatg cggaaggtta cctggatttt
     1201 ttcaaaggcc agataaccga atcccatctc ggcaaggagc tgctggaaaa agtcgagctg
     1261 acggaggata acgccagcaa actggaggag ttttcgaaag agtggaagga cgccaacgat
     1321 aagtggaatg ccatgtgggc tgtcaaaatt gagcagacca aagacggcaa acattatgtc
     1381 gcgggtattg gcctcagtat ggaggacacg gaggaaggca aactgagtca gtttctggtt
     1441 gccgctaacc gtatcgcgtt tattgacccg gcaaacggga atgaaactcc gatgtttgtg
     1501 gcgcagggca accagatatt catgaacgac gtgttcctga agcgcctgac ggcccccacc
     1561 attaccagtg gtggcagtcc tccggtattt tccctgacat cagacggaaa gctgaccgct
     1621 aaaaatgcgg atatcagcgg taacgtgaat gcgaactccg ggacgctcaa caacgtcacg
     1681 attaacgaga actgccgggt tctgggaaaa ctgtccgcga accagattga aggcgatctc
     1741 gttaaaacag tgggcaaagc tttcccccgg gactcccgtg caccggaacg gtggccatca
     1801 gggaccatta ccgtcaggat ttatgacgat cagccgtttg accggcagat tgttattccg
     1861 gcggtggcat tcagcggcgc taaacatgag agagagcata ctgatattta ctcctcatgc
     1921 cgtctgatag tgcggaaaaa cggtgctgaa atttataacc gtaccgcgct ggataatacg
     1981 ctgatttaca gtggcgttat tgatatgcct gccggtcacg gtcacatgac gctggagttt
     2041 tcggtgtcag catggctggt aaatgactgg tatcccacag caagtatcag cgatttgctg
     2101 gttgtggtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt ataacccaga
     2161 tacgggcgcc agaaatggcg ccttttttat ttgtggagtg aatatggcag tacagatttc
     2221 aggtgtgctg aaagatggtg cgggaaaacc aatacagaac tgcactattc aactgaaggc
     2281 aaagcgtaac agcaccacgg tactggtgaa cacggtggcc tctgaaaatc cggatgaagc
     2341 cgggcgttac agcatggatg ttgagcatgg ccagtacagc gtcaccctgc tggttgaagg
     2401 ttttccgcct tcacatgccg gaaccattac cgtctatgaa ggttccagac caggtacgct
     2461 gaatgatttt ctcggtgcca tgacggaaga tgatgtcatg ccggaggcat tgcgtcgttt
     2521 tgaggcaatg gtggaagaag cggcacgcaa cgccgaagcc gcctctcaga gcgcagcggc
     2581 ggcaaagaaa tccgaaactg cagcggcatc atcgaagaac gcggcgaaaa cctcagaaac
     2641 gaatgcagct aacagcgcac aggcggcagc gtcctcgcag actgcatcgg caaactccgc
     2701 gacagcagcc aaaaaatcag aaaccaacgc gaaaaacagc gagacagccg caaagacgag
     2761 cgaaaccaac gcaaagtcca gccagacggc agcgaaaacc agcgaaacga atgccaaagc
     2821 cagtgaaact gcggcaaaaa acagccaggt tgcagcagcc caaagcgaga gcgcggcagc
     2881 cggttctgcg acttcagcag ctggatcagc aactgctgcg gctaacagcc agaaagctgc
     2941 gaagacgagt gaaactaacg caaagtccag ccagacggca gcgaagacca gcgaaacgaa
     3001 tgccaaaacc agcgaaactg cggcgaaaaa cagtcaggat gcagcagccc aaagcgagag
     3061 tgctgcagct ggttctgcaa gcgcggcggc tgcttctgcc actgcatcag ccaacagtca
     3121 aacagcagca aaaaccagtg aaaccaatgc aaagacaagc gagactgcag cggcgaactc
     3181 ggcgaaagca t
//
LOCUS       DARNVM010000273         3108 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.274, whole genome shotgun sequence.
ACCESSION   DARNVM010000273 DARNVM010000000
VERSION     DARNVM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.274"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            37..1179
                     /locus_tag="SI621_004641"
     CDS             37..1179
                     /locus_tag="SI621_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEI4067251.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1651..1752
                     /locus_tag="SI621_004642"
     CDS             1651..1752
                     /locus_tag="SI621_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067252.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2111..2374
                     /locus_tag="SI621_004643"
     CDS             2111..2374
                     /locus_tag="SI621_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEI4067253.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2374..2514
                     /gene="ykgO"
                     /locus_tag="SI621_004644"
     CDS             2374..2514
                     /gene="ykgO"
                     /locus_tag="SI621_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEI4067254.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2584..2774)
                     /locus_tag="SI621_004645"
                     /pseudo
     CDS             complement(2584..2774)
                     /locus_tag="SI621_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          925 a          675 c          649 g          859 t
ORIGIN      
        1 acgcattaaa atcagataaa catttgaggt attgttatga caaataaaca cccatcattg
       61 ttttctcctt ttatgctgac agaaaaaata aagctacgta atcgtatcgt tatggctcct
      121 atgacaacct ggtcagctaa tcctgatgga accatctctg agcaagaact cgaattttat
      181 aaacgtcgct cacaaaatgt tggtttagta ataacaggat gcacctatgt cacgccaagc
      241 ggcattgggt ttactcatga atttgccgcg tatgacgacc gtttcattaa cagccttgaa
      301 aagttagcag ctgcagccaa aagtggcggt gctccggcta ttttgcagat tttccacgct
      361 ggtaataaag ccatcccgga gcttgtgcca aataacgatg ttattagtgc cagcgcatca
      421 agcgtaaaat ctggtgactt tatgaagaga gtggtacaaa gcagggaaat gactgaaaac
      481 gaaattcagg aaaccattag agcattcgga gatgtcacaa aaagggcgat taaagcaggt
      541 tttgatggcg ttgaacttca tggtgcgcac ggattccttc tacagaattt cttctcccct
      601 ctatttaacc agcgtaatga ccgatgggga ggagatctcg aaagacgtat gcgattcccg
      661 ctggccgtat tacaggaagt aaaaaatgta gtctatgaat atgcgacaaa gccctttgcc
      721 atcggttaca gaatatctcc tgaagaatct gcaacagggg ggctgcggat agaggatact
      781 tataaactcc ttgatcggct cattgcatcc ggtatttcgt atatacatac ctcgctggtc
      841 agtattaacg atagctaccc tgttgagtcg ccaaatggcc cccgaactat tgaattgatt
      901 ttaaatcata ttgcaggcag agtgccggta atcgctgcag gtaaaatacg cacaccgtct
      961 caggctcaag aagcaatttc cactggcctg ccacttgttg cgatcggtaa agggctggtg
     1021 ataaatccag aatgggtgac tttagctgaa tcaggtagaa gccatgaaat tcaaacaacc
     1081 cttaatccac aacttgttcc tgaattaacc attccggata aactatggga tcaaattcag
     1141 gcatcgaaag gaactggctg gtttccgtta atggattaaa tatctattac tctcccgagt
     1201 gtaaaactat caaaccaatg agtaaccgat acagcctcgc aaattctttc agataattaa
     1261 cccgtcactg cggcacgttc tctcataagg cgtgcctgct gtgtaatcca gtatggatcc
     1321 agcatagttg acggcttaat agactcaatt cctttcccag gttaccaaca acgatatctt
     1381 gtataacacc ccagaagata cgacattatg atgagatgag gtatgctcct cagcgccaat
     1441 caacgccagg gctgggccag ttttgagatc agactcattc ccggactgac ttcaggcgac
     1501 atatatcaat gacgttgggg caataaaaga tagccgatgt aatacatcta ttcaaatttg
     1561 tgatacatgt caaaaaattg gttgaccaaa ctcgttattt atataagggc acttacgaag
     1621 tgcactcttt tttaaagcga ggaagtacca atgaaagaga ataaagtaca acaaatcagt
     1681 cataaactga ttaatatcgt tgtttttgtc gcaattgtag aatacgccta tttatttctc
     1741 catttctatt aataacggca ataaactgtt cacttcagtg atattaaaaa atatgcatcc
     1801 tctccctttt ttgtaagtaa ttattatatc cgtgggagag gaatacacgc tgtccggcaa
     1861 tcaatcaaac tgcgataaat catcggccag taaaatggag atagcctcca ttctcgaaaa
     1921 attcatactc tcagcgaaac catcactcat caaggcgttt atgggagcgt cgccaatggc
     1981 tgctaacaat gccagacttc cccgttgccg aaattccaca taccacaaat ggtcacaggg
     2041 attgggtgtt gaaatgatcc ggatgagcat gtatctttat ggttatgtta taacataaca
     2101 ggtaaaaatg atgaagccca atatccatcc tgagtatcgt actgtggtgt tccacgacac
     2161 cagtgttgat gagtacttta aaatcggctc gactatcaaa acagaccgtg agattgagct
     2221 ggatggcgta acgtatccgt acgtgacaat tgatgtctct tctaaatcgc acccgttcta
     2281 tacagggaag ctgagaacag tggcatcaga aggaaatgtt gcacgattca cccaacgttt
     2341 tggtcgtttt gttagcacga aaaagggggc atgatgaaag tccttaactc tctgcgtacc
     2401 gcaaaagaac gccatccaga ctgtcagatt gtgaagcgaa aaggacggct atatgtgatt
     2461 tgtaaatcta atccacgttt taaggccgtt cagggtcgta agaaaaaacg ttgattcaaa
     2521 attcgacgaa ttaacgatat ttgtctgatt aataatcaga ccggattaat gttggtgtat
     2581 ttattacacc agcattaatt ttcctgggga tgtatcttcc tgttcattta aagtcaactg
     2641 cctgacgttt ctctccgaat attccattat attaatgttg acttgttgac caggtgcgcc
     2701 ctgtatgctg gcatcaactc tcttttagac tgaacacgcc actcagtttc ctccctttgc
     2761 gacgcagcct gcatttacac tcaaacggtt aagatgataa atgtggtaaa tctgttggta
     2821 ctgacataaa aacgtttacg ccacaggaac ggcctgatcc accggtaacc ccatcgccga
     2881 cgttcgagtg ccagttagag taacgcgcac agatgactga atgcagtgcc ctggcaaaga
     2941 ggccatcgtt cctgtgacaa ctggcagtct tcgtttaact tcacttaatt tggctcttag
     3001 ggggcttacc ggacagatga cgtacttaca cctgtttaat ttttcatcac ttattgggat
     3061 gaacccccat aaccattttg tgcggcatgt taatccatta aaacacct
//
LOCUS       DARNVM010000274         3094 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.275, whole genome shotgun sequence.
ACCESSION   DARNVM010000274 DARNVM010000000
VERSION     DARNVM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.275"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            56..199
                     /locus_tag="SI621_004646"
     CDS             56..199
                     /locus_tag="SI621_004646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067255.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            complement(426..1964)
                     /gene="aldB"
                     /locus_tag="SI621_004647"
     CDS             complement(426..1964)
                     /gene="aldB"
                     /locus_tag="SI621_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HEI4067256.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMELVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2165..>3094)
                     /gene="yiaY"
                     /locus_tag="SI621_004648"
     CDS             complement(2165..>3094)
                     /gene="yiaY"
                     /locus_tag="SI621_004648"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HEI4067257.1"
                     /translation="ENVAAGLKLLKENNCDSVISLGGGSPHDCAKGIALVAANGGDIR
                     DYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMAIVDKHVTPLLSVND
                     SSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMIAENLPLAVEDG
                     SNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAVLLPHVQVFN
                     SKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAGLRDLNVKEEDF
                     AVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT          736 a          855 c          763 g          740 t
ORIGIN      
        1 ttttcaacag tgcgaggctt atcatattga aataatggaa ctattggcca aactaatgaa
       61 taactccagt aaaacatcca cagtacagat taagcgtatt aaaccttcaa ttatctaccg
      121 tttattgctg attggcctcg gaacgccaat ggtgatttat ggtctggttc gcccgctcac
      181 catcgaaacg cgagattaaa ctcctggcaa aacacgcccc agaaacgcac ttctgttggc
      241 gtagtctggg ttattgggtt attgcgcacg taggtttaaa acgtaaattg taacaacgtg
      301 cgctttgttt atgccggatg cggcgtgtac gccttatccg gcctacggtc aggtccccgt
      361 aggcatgata aaacgcgtaa gcgtcgcatc aggcaatgaa tacccaatgc gaccagcttc
      421 ttatatcaga acagccccaa cggtttatcc gaatagctca ccagcaggca cttggtttgc
      481 tggtaatgct caagcatcat cttatgggtt tcgcgtccga tgcccgactg tttgtagccg
      541 ccaaacgccg catgtgccgg gtaagcgtga taacagttgg tccacacgcg cccagcctga
      601 atgccgcgcc ccatcttata ggccagatta ccgttgcggc tccagacacc cgcgcccagg
      661 ccatattgcg tgtcgttcgc cagctccagc gcttcttcca tcgttttgaa ggtggtcacc
      721 gccagcaccg gaccaaaaat ttcttcctgg aacacgcgca tattattctg accaaacaga
      781 atcgtcggtt cgaggtagta gccgtctttc agttcgcctt ccagcagctt gcgccgcccg
      841 ccggtgagca cgtctgcgcc ctctttttta ccgatatcaa tgtagttgag gatggtttcc
      901 agttgcccgt gagaaacctg cgcccccatt tgcgtcacgc tgtcgagcgg gttaccgctg
      961 cgaatgcttt cgacacggcg gatggcgcgt tccataaagc gttcgtagat agactcctgc
     1021 actaaagcac ggcttggaca ggtgcaaact tcgccctggt taaaggcaaa cagtgcaaag
     1081 ccttccagcg ctttatcgaa aaaggcatct tcttcatcca tcacatcagc aaagaagata
     1141 tttggggatt tgccgcccag ctccagcgtc accggaataa tgttttgcgt ggcgtactgc
     1201 ataatttgtt ggcccacttc cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag
     1261 gtcgccagat attcaccaat ttccccacca gcgccgttaa ccacgttcac cacgcccggc
     1321 ggcagtaaat caccgacgag ttccattagc agcagtacag aaagcggggt aagacgtgcg
     1381 ggtttcagca ccacacagtt gcccgccgcc agcgcgggag ccattttcca gctcgccatc
     1441 agcagcggga agttccacgg aataatctgc cccaccacgc ctaacggttc gtggaagtga
     1501 taagccacag tttcgctatc cacttcacta ataccgcctt cctgcgcccg aatacacgag
     1561 gcaaaatagc gaaaatggtc aatcgccagc ggcacatcag cagcactggt ttcgcgaatg
     1621 ggtttgccgt tatcccaggt ttcagcggtc gctaacagtt cgaggttctg ctccatccga
     1681 tcggcaatct taaacagaat cgccgcgcga tcctgcaccg aagtatgcgc ccatttatct
     1741 ttcactttgt gcgcagcatc cagcgccaga tcgatgtctc gtttgcccga agacgccact
     1801 tcgcacagca gctgcccggt caccggcgtc aggttctggt aatactcacc atcggcaggg
     1861 gctacccatt cgccgccaat aaagttgtcg tagcgggctt ttaacttgag ggggaaacca
     1921 tactcgccgg gcttaatctg tgctgaaggg ggattattgg tcatgatcgt ctccttgcgg
     1981 tgtgaggtat aacaagggta gacgtgactg gcgaaatctt cgccagtcgg taacagcttt
     2041 acgacggcta tcacgaattt acgggcaagg attacagaga taactaaaac tctctcaaaa
     2101 atcgcaaatt ttgagatagt taaaaaaatg gctgacaatt agccagccat tgggggaatg
     2161 atgattacat cgcagcgcga taaattgcca caatctcttc gtgagttgcc tgaatcgggt
     2221 tagtaaaccc acacgcatct ttcagggcat tggtggccag taccgcgaaa tcctcttctt
     2281 tcacgttcag gtcacgcagg cctgccggga tatccacttt cttcgccagt tcacggatgg
     2341 cattgatgca cgcttctgcg ccttccgcgt catttttgcc tgtcacgtta acgcccatgg
     2401 cagcagcaca gtcacgcaga cgcgcagcag cgactttgct gttgaacacc tgaacgtgtg
     2461 gcagcagaac ggcgttacat acaccgtgcg gcagattgta gaaaccgccc agctggtgcg
     2521 ccatcgcatg aacataaccc agagaagcat tattgaacgc cataccggcg aggaactggg
     2581 cataagccat tgcttcacgc gctttcgcat tactgccatc ttcaacggct aacggcaggt
     2641 tttcggcaat catggtcacg gctttcagtg cacaagcgtc agtgatcggc gtggcggcaa
     2701 tagaaacata cgcttcgata gcgtgcgtta aggcatccat acccgttgcg gcggtcagtg
     2761 acttcggcat accaatcatc agagaggagt cattgacaga aagcagcgga gttacatgtt
     2821 tatccacaat cgccattttg atgtgacgcg cttcgtcagt gatgatgcag aaacgggtca
     2881 tttcagaggc cgtacccgcc gtggtgttga tggcgatcat cggcagctgc ggttttgcag
     2941 agcggtcaac gccttcataa tcgcgaatat cgccaccatt ggctgccacc agcgcaatac
     3001 cttttgcgca gtcgtgtgga gaaccaccgc ctaaggaaat cacgctatcg caattatttt
     3061 ctttaagtaa tttcaaacct gcagcgacgt tttc
//
LOCUS       DARNVM010000275         2940 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.276, whole genome shotgun sequence.
ACCESSION   DARNVM010000275 DARNVM010000000
VERSION     DARNVM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.276"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            66..2345
                     /gene="ydeP"
                     /locus_tag="SI621_004649"
     CDS             66..2345
                     /gene="ydeP"
                     /locus_tag="SI621_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HEI4067258.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLNWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2593..2790
                     /gene="safA"
                     /locus_tag="SI621_004650"
     CDS             2593..2790
                     /gene="safA"
                     /locus_tag="SI621_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HEI4067259.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2865..>2940
                     /locus_tag="SI621_004651"
     CDS             2865..>2940
                     /locus_tag="SI621_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="regulates the cellular response to acid resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEI4067260.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAF"
BASE COUNT          803 a          669 c          705 g          763 t
ORIGIN      
        1 ttgtatatac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
       61 agcaaatgaa gaaaaaaatt gaatcctacc agggagctgc cggtggttgg ggtgctgtta
      121 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccaggatgtt attgccatgt
      181 ttgacatgaa taagccagag ggctttgact gtccgggatg tgcatggcca gatcctaagc
      241 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
      301 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttaactggg
      361 gagatcacga gcttgaggct gcggggcgtc tcacccagcc tttgaaatat gatgccgtca
      421 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gaacgccttc
      481 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
      541 ctgcctttct ttatcagctt tttgctcgtg aatacgggag caataacttc cccgactgct
      601 ccaacatgtg ccatgaaccg accagtgtgg gtttggcagc gagtatcggt gtaggtaaag
      661 ggaccgtgtt gctggaagac tttgagaaat gcgatttagt catttgcatt gggcataacc
      721 ctggtacaaa ccaccctcgc atgttgactt cgttgcgcgc tttagtgaaa cggggagcga
      781 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
      841 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
      901 gcattggtgg tgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
      961 atgctgcaag cgccgcaggt cggccctcat tgctggatga cgaatttatt caaacgcata
     1021 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgagc
     1081 gtatttctgg actaagtcag actcaaatcg ccgaactggc tgatgcatat gccgctgccg
     1141 aacgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     1201 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     1261 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     1321 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggtttcacc ccacctcatg
     1381 cacctggaca tgcagcaatt gccagcatgc aagcaatatg tacggggcag gctcgagcat
     1441 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagca agcgctgtac
     1501 cgttaacaca attagatctg acggtacacg tagccactaa gcttaaccgc tctcatctgc
     1561 tgaccgcacg gcatagctat attctgccgg ttctgggacg tagcgagatt gacatgcaaa
     1621 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     1681 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggtatcg
     1741 cgcaggcggc actaccccag agcgtggtag cctgggagta tctggtggaa gattatgatc
     1801 gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgactataac cagcgcatcc
     1861 gacatcctgg aggttttcac ctgataaacg cagctgctga aaggcgctgg atgacgccgt
     1921 caggtaaggc taatttcatt accagtaaag ggctgttaga agatccctct tcagcgttta
     1981 acagtaagct agtcatggcg acagtacgca gtcacgatca gtacaacacg acgatttatg
     2041 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     2101 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     2161 cagacggtaa gcgaagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     2221 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca cttgataacc
     2281 acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta gaaccatcaa
     2341 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat tcctcattga
     2401 ccattgtcgc gcaatttact cacatgtgct aaattaaact gctcgttata agatatatac
     2461 ctccgcctac agctgtaaga aactccgctc agtactgaag cgccagtcct atttcctcta
     2521 ttctccagcc tgttatatta agcatactga ttaacgatga ttaacgtcat tcgtttaata
     2581 aacacatttg aaatgcatgc gaccatagtg aaaaataaaa tcacgcaaag agacaactat
     2641 aaagggatca tgtctgtaat tgtggttgtc ttattactga cccttacatt gatagccatt
     2701 ttttcggcaa ttgatcagct gggtatttca gaaatggggc gcattgcaag ggatcttaca
     2761 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     2821 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     2881 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
//
LOCUS       DARNVM010000276         2876 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.277, whole genome shotgun sequence.
ACCESSION   DARNVM010000276 DARNVM010000000
VERSION     DARNVM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.277"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            258..1424
                     /gene="nhaA"
                     /locus_tag="SI621_004652"
     CDS             258..1424
                     /gene="nhaA"
                     /locus_tag="SI621_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEI4067261.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1490..2389
                     /gene="nhaR"
                     /locus_tag="SI621_004653"
     CDS             1490..2389
                     /gene="nhaR"
                     /locus_tag="SI621_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEI4067262.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2427..>2876)
                     /locus_tag="SI621_004654"
     CDS             complement(2427..>2876)
                     /locus_tag="SI621_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HEI4067263.1"
                     /translation="NTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQ
                     GEQGELVFEPSDVEYYFEPVTIQDSGTSILKNDLENSEDGAGQIGFQLSNDGTHEMQY
                     GKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          691 a          631 c          716 g          838 t
ORIGIN      
        1 cgccgactga caacaaatta attattactt ttcctaatta atccctcaga aatcctgacc
       61 ttaagctatg attatcaggg tttagggtca ctcgtgagcg cttacagccg tcaaaaactc
      121 atctcatcgc tgatggcaca aatccttcaa tggttcgtaa aaaacgaatt attcttacac
      181 tataatctga ttttaacgat gattcgtgcg gggtaagata gtaaaaacga tctattcacc
      241 tgaaagagaa ataaaaagtg aaacatctgc atcgattctt tagcagtgat gcctcgggag
      301 gcattattct tatcattgcc gctatcctgg cgatgattat ggccaacagc ggcgcaacca
      361 gtggatggta tcacgacttt ctggagacgc cggttcagct ccgggttggt tcactcgaaa
      421 tcaacaaaaa catgctgtta tggataaatg acgcgctgat ggcggtattt ttcctgttag
      481 tcggtctgga agttaaacgt gaactgatgc aaggatcgct agccagctta cgccaggctg
      541 catttccagt tatcgccgct attggtggga tgattgtgcc ggcattgctc tatctggcct
      601 ttaactatgc cgatccgatt acccgcgaag ggtgggcgat cccggcggct actgacattg
      661 cttttgcact tggtgtgctg gcgctgttgg gaagtcgtgt tccgttagca ctgaagatct
      721 ttttgatggc tctggctatt atcgacgatc ttggggccat cattatcatc gcattgttct
      781 acactaatga cttatcgatg gcctctcttg gtgtcgcggc tgtagcaatt gcggtactcg
      841 cggtattgaa tctgtgtggt gtacgccgca cgggcgttta tattctggtt ggcgtggtgt
      901 tgtggactgc ggtgttgaaa tcgggggttc acgcaaccct ggcgggggta attgtcggct
      961 tctttattcc tttgaaagag aagcatgggc gttctccagc gaagcgactg gagcatgtgt
     1021 tgcacccgtg ggtggcgtat ctgattttac cgctgtttgc atttgctaat gctggcgttt
     1081 cactgcaagg cgtcacgctg gatggcttga cctccattct accattgggg atcatcgctg
     1141 gcttgctgat tggcaaaccg ctggggatta gtctgttctg ctggttggcg ctgcgtttga
     1201 aactggcgca tctgcctgag ggaacgactt atcagcaaat tatggcggtg gggatcctgt
     1261 gcggtatcgg ttttactatg tctatcttta ttgccagcct ggcctttggt agcgtagatc
     1321 cagaactgat taactgggcg aaactcggta tcctggtcgg ttctatctct tcggcggtaa
     1381 ttggatacag ctggttacgc gttcgtttgc gtccatcagt ttgacaggac ggtttaccgg
     1441 ggagccataa acggctccct tttcattgtt atcagggaga gaaatgagca tgtctcatat
     1501 caattacaac cacttgtatt acttctggca tgtctataaa gaaggttccg tggttggcgc
     1561 agcggaggcg ctttatttaa caccacaaac cattaccgga cagattcggg cgctggaaga
     1621 gcgcctgcaa ggcaaattat ttaaacgcaa gggacgtggt ctcgaaccca gcgagctggg
     1681 agagctggtc tatcgctatg ccgataaaat gttcacctta agccaggaaa tgctggatat
     1741 cgtgaactat cgcaaagaat ccaatttatt gtttgacgtt ggcgtggctg atgcactttc
     1801 taaacgcctg gtcagtagcg tacttaacgc cgcagtggta gaaggcgagc cgattcatct
     1861 tcgctgcttc gaatccaccc acgaaatgct gctggagcaa ttaagtcagc ataaactgga
     1921 tatgatcatt tccgactgtc cgatagattc tacgcagcag gaaggtctgt tctccgtgag
     1981 aattggcgaa tgtggcgtga gtttctggtg tacaaatcca ccaccggaaa aaccgttccc
     2041 ggcttgtctg gaagaacggc ggcttttgat tcctgggcga cgttcaatgt tagggcgcaa
     2101 attgcttaac tggtttaact cccagggatt aaacgtagaa atcctcggcg agtttgatga
     2161 tgccgcgttg atgaaagctt ttggtgcgat gcacaatgca atcttcgttg ccccaacgct
     2221 ttatgcatat gacttttatg ccgataaaac tgtcgtagaa attggtcgcg tcgaaaatgt
     2281 gatggaagag taccatgcta tttttgctga gcggatgatt cagcacccgg cggtacagcg
     2341 aatctgcaat acggattatt ctgcgctttt tagcccagcg gcacgttaat cggcagctcc
     2401 cccaaagtta aggtggggga gatagattag ttgtacatta ccacgatttt gactcggctc
     2461 attatttgcc cgcttgagac attatttcca tatgtacgcg ggcgaataaa tagaggaatc
     2521 tgattacttc cttcatgggg atgctgaaaa gagtagtaat tgcttttacc atactgcatt
     2581 tcatgcgtac catcattgga gagttgaaag cctatttgac ccgcaccgtc ctcactgttt
     2641 tctaaatcat tttttaatat gctggttcct gaatcctgta tcgtcactgg ctcaaagtaa
     2701 tattcaacat cactaggttc aaaaactaat tcaccttgct caccctggat acagtcaaat
     2761 cttatatgat gaaactgata attttcatta ttgatacgag acagtacaaa tcgtccagtg
     2821 tcgatggatt gatcttcaaa accacaggtc attaaattta cctgtaaaat agtatt
//
LOCUS       DARNVM010000277         2870 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.278, whole genome shotgun sequence.
ACCESSION   DARNVM010000277 DARNVM010000000
VERSION     DARNVM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.278"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            102..827
                     /locus_tag="SI621_004655"
     CDS             102..827
                     /locus_tag="SI621_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEI4067264.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            785..1462
                     /gene="bglJ"
                     /locus_tag="SI621_004656"
     CDS             785..1462
                     /gene="bglJ"
                     /locus_tag="SI621_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HEI4067265.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1500..2288)
                     /gene="fhuF"
                     /locus_tag="SI621_004657"
     CDS             complement(1500..2288)
                     /gene="fhuF"
                     /locus_tag="SI621_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HEI4067266.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSTNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2429..2665
                     /locus_tag="SI621_004658"
     CDS             2429..2665
                     /locus_tag="SI621_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HEI4067267.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLKQG"
     gene            complement(2704..2790)
                     /locus_tag="SI621_004659"
     tRNA            complement(2704..2790)
                     /locus_tag="SI621_004659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2754..2756),aa:Leu,seq:cag)
BASE COUNT          751 a          634 c          722 g          763 t
ORIGIN      
        1 gataatttat tcgcttaatc tattaatttg ctggaaacat ttaaggaatg aatatcctga
       61 ataaaccatc gtaattttta gacagtggat gtggaggaaa tatgttgcca ggatgctgca
      121 aaaatggaat tgttatcagt aaaatacctg ttatgcaagc agggttaaaa gaggtcatga
      181 ggactcactt ccctgaatat gaaataatat ccagcgcctc tgcggaggac cttaccttat
      241 tacaattacg tcgttccgga ttagtcattg ctgatttagc cggtgaaagt gaagatccac
      301 gttctgtttg tgaacattat tattctttaa tctcgcaata tcgggaaatt cactgggttt
      361 tcatggtgtc acgctcctgg tattcccagg cagtagaact gcttatgtgc cctacggcga
      421 cgttattgtc tgatgttgaa cccattgaga atctggtcaa gaccgtacgt tccggcaata
      481 cgcacgcaga gcgtatcagc gccatgctga cctctccggc aatgactgaa actcatgatt
      541 ttagctatcg ctccgtcatt ctcactcttt cagagcgcaa ggtactgcgg ctattaggta
      601 aaggatgggg catcaaccag atagcttcat tgcttaagaa aagtaataaa actatcagcg
      661 cccaaaaaaa cagtgcgatg cgtcgactgg caattcacag caacgctgaa atgtatgcat
      721 ggataaatag cgcgcagggt gcaagagaac ttaacttgcc ttctgtttat ggagatgccg
      781 cagaatggaa cacagccgaa ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat
      841 gagtagtatc ggtattgaga gtttattcag aaagtttgcg ggtaaccctt ataagctcca
      901 tacctatacc agtcaggagt catttcagga tgccatgtcg cggatctcgt ttgcggcggt
      961 cattttttct ttttctgcca tgagaagtga gcgcagagag ggattatctt gcctgactga
     1021 actggcgatt aagtttccgc gtacccggcg tttagttatt gcggatgatg atattgaagc
     1081 tcggctgatt ggttcattgt cgccatcacc gttggacggt gtattaagta aagcgtcaac
     1141 gctggagatt tttcatcagg aactcttttt gtcattaaat ggtgtacgtc aggcgaccga
     1201 ccgactgaac aatcagtggt acattaatca aagccgaacg ctaagcccga cggagagaga
     1261 aatattgcgc tttatgtcgc gtggctactc aatgacacaa attgccgagc agcttaaacg
     1321 caatatcaaa acgatccgtg cacataaatt taatgtgatg tcgaaactgg gcgtcagttc
     1381 tgatgcaggg ttgttggagg ccgcagatat tctgttatgt atgcggcatt gcgaagcaag
     1441 taatgtgttg catccctatt aatccgcatg atgccgggtt tacttccccg gcagtgcttt
     1501 catttcagcg tacaatcgcc acattgctgc acatccggta agcgataacg ctggcagcaa
     1561 gtgcggcgca ccagcaggcc gtcgcgcagt accacggtac gccagagtgg attatcttca
     1621 ccgttcgtga gcattttctc aaaaaagagg gcatggcgca gcgattcaac cgtcgcctcg
     1681 ccaagcagct gcttcatctc agtgagatac cagttgatca aataaccggt attactccag
     1741 ataagtttgc cgttgatctc tcccgtcgct tctaatgcct gcacaaccgg aaccagcgcc
     1801 tggctgatta acgtttccat tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca
     1861 cacacatcga cccagaaaca ggcggcgcgt ccggtttcgt gaaactcaac gtggaaatgt
     1921 tccggcgaca catctaatgc tttttcctgc gtcagcagcg ccaacattaa tggtggcacc
     1981 atcaggccga tataccattg cgcccatagt gagatcagcg gtttgttctc gcggatcatc
     2041 gttggttggt tgcgatagat atgatcggaa tagaccgcga gtagagaact tagcgcattc
     2101 gttgatgacc attgcgccag cgtcatggcg ttaagcggag cgggttcatc caggcggata
     2161 aactcgagca aatgttcacg atgttcggcg atcgtcgccc gtacggcttg tgcaagccca
     2221 gcatcctgcg gctggagatg cgttcgccag ataacatctt catagagcgg tgcggaacga
     2281 taggccataa tcgggatagt aatctaaatg ataatgattg ctaatcctag cgataggttt
     2341 acccgatagc aagggatttc tctggcttgc aaatgataaa aattatcata tgatattggt
     2401 tatcattatc aatgaaagag atgaaaccat gttgcaacgt acgctgggca gtggctgggg
     2461 agtgttgctg ccgggattgc taattgcagg gctgatgtat gcggatttat cgccggatca
     2521 gtggcggatt gtcattctga tgggattagt attgacgccg gtgatgctgt atcacaaaca
     2581 gttgcggcat tacattttgc tgccatcgtg cctggcgctt attgctggca tcatgctgat
     2641 gataatgaat ttgaagcagg gatgataaaa cagggaagca acaagaaagg aagtaaagat
     2701 aattggtgcg agggggggga cttgaacccc cacgtccgta aggacactaa cacctgaagc
     2761 tagcgcgtct accaattccg ccaccttcgc acagtcatct tacttttttt gatatcgcct
     2821 cgtttggtgc gagggggggg acttgaaccc ccacgtccgt aagaacacta
//
LOCUS       DARNVM010000278         2688 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.279, whole genome shotgun sequence.
ACCESSION   DARNVM010000278 DARNVM010000000
VERSION     DARNVM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.279"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="SI621_004660"
     CDS             complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="SI621_004660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEI4067268.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            918..1385
                     /gene="rnhA"
                     /locus_tag="SI621_004661"
     CDS             918..1385
                     /gene="rnhA"
                     /locus_tag="SI621_004661"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEI4067269.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1382..2104)
                     /locus_tag="SI621_004662"
     CDS             complement(1382..2104)
                     /locus_tag="SI621_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEI4067270.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2138..>2688
                     /gene="gloB"
                     /locus_tag="SI621_004663"
     CDS             2138..>2688
                     /gene="gloB"
                     /locus_tag="SI621_004663"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEI4067271.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLS"
BASE COUNT          705 a          664 c          640 g          679 t
ORIGIN      
        1 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
       61 ttttgaatcg tttgctgcaa aaatcgccca agtcgctatt tttagcgcct tttacaggta
      121 tttatgcccg ccagaggcag cttccgccct ttttctgcac cagatcgaga cgggcttcat
      181 gtgccgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgcacaa
      241 tgcgctgaat tgttgtttca ccttgttgct gttgtgtctc tccttccatc gcaaaagcca
      301 tcgacgtttg accaccagtc atcgccagat aaacttccgc aaggatctgg gcatcgagta
      361 atgccccgtg caacgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      421 ggctgttgcg cttaccggga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      481 agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc
      541 cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcgaatatag tccatgaact
      601 catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      661 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      721 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
      781 agtgcgcgcc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
      841 ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta
      901 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
      961 gtaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct
     1021 ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1081 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1141 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca
     1201 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcgctg gggcagcatc
     1261 aaatcaaatg ggaatgggtt aaaggccatg ccggtcatcc agaaaacgaa cgttgcgatg
     1321 aactggctcg tgccgcagcg atgaatccca cactggaaga tacgggctat caagtcgaag
     1381 tttaagcctg tggtttacga cattgccggg tggctccaac cgcctggcga attcgtggct
     1441 tgtttttact ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcgacaa
     1501 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcca ccgtgtttgt
     1561 tccacgggag aacgtggaaa cggctggcgt gtagcacttc aaaattcaac aaagagagcc
     1621 agtccagctg ccgcatcaga gtaaacatcc ggctattata gggcgaggtt ttgcgcaata
     1681 ccggcacaag ttttcgtaat cccattaaac tgatgggatt gaagccacta atcaccagcc
     1741 agccatcatc aatcaatacc cgatcggctt cacgcaataa acgatgcgga tcggtgcacc
     1801 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     1861 gtgggtccgc ctgcacctgg acgggcagtc cttgcgcaga aacattcact tgatgagaaa
     1921 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac
     1981 catacatttt agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc
     2041 cccagggcaa atcgccccag caatcaggag ccacgacagt ttgagggact cttgccggtt
     2101 tcatcacaac cttccgtttc acactgagag gtaatctatg aatcttaaca gtattcccgc
     2161 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga
     2221 tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc
     2281 catatttctc acccaccatc accacgatca cgttggcggc gtaaaagaac tggtgaaaaa
     2341 gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt
     2401 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc
     2461 gggtcacact ttaggacaca tctgttactt cagtaaacct tatctatttt gcggcgacac
     2521 gctgttttct ggtgggtgtg gtcggttgtt tgaagggaca ccatcacaaa tgtatcaatc
     2581 acttaaaaag ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac
     2641 cttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtc
//
LOCUS       DARNVM010000279         2611 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.280, whole genome shotgun sequence.
ACCESSION   DARNVM010000279 DARNVM010000000
VERSION     DARNVM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.280"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            483..2006
                     /locus_tag="SI621_004664"
     CDS             483..2006
                     /locus_tag="SI621_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEI4067272.1"
                     /translation="MRNTLIPIFIAICLFITGVTILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2138..2356
                     /locus_tag="SI621_004665"
     CDS             2138..2356
                     /locus_tag="SI621_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067273.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITILVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQQ"
BASE COUNT          723 a          535 c          567 g          786 t
ORIGIN      
        1 attgatcagg ctttacgctt gcgtttaaaa gatgaaacta gtgtgattct ttctgatctc
       61 tatctgcaaa tcttgcagta cattgaaatg cataagacta cactaacgga tatcattatt
      121 aatgacagtg aatcagtgct ctcttagaac ttgagtaagg ttatagaata aacatcagct
      181 gtatcatcat gctggtgcaa agtgagcatt tcaggcgtta tgctttctta atatatccgc
      241 aatatcaggg ggcaaacagg aatggagagc gctcgttttc cattcttgac gcctgatatc
      301 ccacctaatt tgtttaattc tgactacatt gtgctcaata attaataggt tagatgaaga
      361 tggagaaaat tttatcttcg gcgtggatat ttcttatgct ctctaccatg ctctctgtag
      421 atgccagtgc gagcgaatga atggcatttt catgtattaa accctttggc cgggacaagc
      481 aaatgcgcaa tacactcata cccatcttca ttgcgatatg cttgtttata accggtgtca
      541 ctattttgaa catccagctc tggtattccg caaaagcaga gtacctggcg ggagcaagat
      601 atgcagccaa caatatcaat catatacttg aagaagcgtc acaggcgact caaacggcgg
      661 ttaacattgc cgggaaggaa tgcgacctcg aggagcaata tcagcttggc actgaagcag
      721 ctctgaaacc tcacctgcgc acaatcatca ttctcaaaca gggaacggtc tggtgtacat
      781 ccttaccggg gaatcgggtc ctactgtctc gtattcctgt tttcccggac agtaatttac
      841 tgctggctcc ggcaatcgac accgttaata gattacctat cctgctctat cagagccaat
      901 ttgcagatac gcgcattttg gttacgataa gtgatcagca tattcgcggg gcgcttaatg
      961 tacccttgaa aggggtaagg tatgtattac gcgtggcaga tgacattatt ggacctacgg
     1021 gtgatgtgat gacgcttaat ggacactatc cctataccga gaaggttcac tccacaaaat
     1081 atcatttcac tattatcttt aacccgccgc cactctttag cttctacaga cttatcgata
     1141 aaggctttgg gattttgata tttattctgt taatcgcctg cgccgctgcc ttcctgcttg
     1201 ataggtattt caataaaagc gcaacgcctg aagagatcct gcgacgggct ataaataatg
     1261 gggagatcgt cccattttac caacctgtgg taaatggtcg ggaagggaca ttgcggggag
     1321 ttgaggtgtt agcccgctgg aaacaacctc acggtggata tatatcaccc gcggcattta
     1381 ttccacttgc tgaaaaatcc ggattaatcg ttccgcttac gcaaagcctg atgaatcagg
     1441 ttgccagaca gatgaacgct atcgcgagta aactgccgga aggttttcat attgggatta
     1501 attttagcgc ctcgcatatt atttcgccga catttgtcga cgagtgctta aattaccgtg
     1561 acagttttac ccgtcgcgat ttaaacctcg ttctggaagt taccgagcgt gagccattaa
     1621 atgttgatga aagtctggtt cagcggttga acattctgca tgaaaatggt tttgtcatcg
     1681 cgctggatga tttcggtact ggctactcag ggctttctta tctgcatgac ctgcatattg
     1741 attatatcaa aattgatcac agtttcgttg gccgcgtaaa cgcagaccca gaatcaactc
     1801 ggattctgga ttgtgtattg gatctggcgc gtaaactttc gatcagtatc gtcgctgaag
     1861 gtgtcgaaac gaaagaacaa cttgactatc tgaatcaaaa taatatcaca tttcagcagg
     1921 gttattattt ctataaaccg gtaacataca tcgacctggt caaaattatc ctttctaaac
     1981 cgaaggtgaa ggttgtggtt gagtaaaaat cgatcagtaa ggccatagtg cggtgtaatt
     2041 atagacagct aattagctcg ttgcctcttg ttactattgt tcattatttt gtttgctata
     2101 attgtttgaa agttttgaca ggattgccat tagtagcatg aacaatagta ataatctgga
     2161 ttatttcact ctctatatca tcttttccat tgcatttatg ctgatcacta tcctggtcat
     2221 ccttattgca aaacccagta ccgggctggg agaagtgctt gtgacgataa atttgcttaa
     2281 tgcccttgtt tggctggcga tcaatctgat taatcgatta agggaaagac tcgtcagcca
     2341 cagggatcag caataatctt tcagtttctc actgtcagta tgcggctgaa tggggtgctg
     2401 acagtgaacg cctggatcat tgaaggaaag gaattattgc gcaaatagtt gtcaaccctg
     2461 gtgttatcac agttggtttt atatatcatc gaaataattt ctatggcaac tattaataat
     2521 tttgatgtcg aagagttatt tgttaaacaa aatcgtcacc ccaaagtgat caatgtcatg
     2581 aaaataaggt gaaaaatgat aatgccgcct t
//
LOCUS       DARNVM010000280         2605 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.281, whole genome shotgun sequence.
ACCESSION   DARNVM010000280 DARNVM010000000
VERSION     DARNVM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.281"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            439..582
                     /locus_tag="SI621_004666"
     CDS             439..582
                     /locus_tag="SI621_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HEI4067274.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            595..804
                     /gene="cspE"
                     /locus_tag="SI621_004667"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             595..804
                     /gene="cspE"
                     /locus_tag="SI621_004667"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HEI4067275.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            970..1779
                     /gene="rlmA"
                     /locus_tag="SI621_004668"
     CDS             970..1779
                     /gene="rlmA"
                     /locus_tag="SI621_004668"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HEI4067276.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="SI621_004669"
     CDS             complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="SI621_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HEI4067277.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          688 a          609 c          623 g          685 t
ORIGIN      
        1 gtttctctct tgattttttt gcgcattcat caatttttct tattttaaat ttacaatacc
       61 tttggcgatt gacttctctt tggggtgatc gatagccgtt aactgactgt tttatgagaa
      121 aaagtgatat aactttttat tcattgcata gcagaaaatg tgatattgca cgcactatgt
      181 aataacttct cccactggcc tggaacaact gaacttattg aactatgtta gaaaatacgt
      241 cagtttaagt atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc
      301 cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga
      361 catgtttaaa aatggcttgc cataattaac gttgtatgtg ataacagatt tcgggttaaa
      421 cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt
      481 gacgttgaat accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca
      541 gttttatcta tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca
      601 aagattaaag gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg
      661 gctgatggca gcaaagacgt gttcgtacac ttctctgcaa tccagggtaa tggtttcaaa
      721 actctggctg aaggtcagaa cgttgagttc gaaattcagg acggtcagaa aggtccggca
      781 gctgttaacg taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa
      841 tctcgctata aagcctcgtc gaatgcgagg ctttttactt tgctttatct tcgctccagt
      901 cgttcggata tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgcc
      961 ggagttgtca tgtcgttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac
     1021 agctatatct gtccccagcg acatcagttt gatatggcga aagaagggta tgtcaatctg
     1081 ctgcccgttc agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca
     1141 cgccgcgcat tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgctcaa
     1201 ctgcgggaac ggcttgatga aaaggccaca gcggtgctgg atattggctg tggtgaaggg
     1261 tattacacac acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg cctggatgtc
     1321 tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc aggtcacttt ttgtgttgct
     1381 tccagccacc gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg
     1441 ccgtgtaaag cagaagaatt agcgcgagta gtgaagcctg gcggttgggt cattactgcc
     1501 acgccgggac cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt
     1561 catgcacctc atgcagaaca actggaaggt tttacattac agcagagtgc tgagttgtgt
     1621 tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc
     1681 tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag
     1741 acggacttta atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg
     1801 atctggacgc cgatgccgat cagcaccagc ccgccgagaa tttccgcttt tttcccaata
     1861 attgagccga taaagcgacc aaccatcatc cctaatgtcg acataatcaa ggttgcacaa
     1921 ccaatggcca atgcggtcgc gataatgttg acctgaagga aagcaagacc aacacccaca
     1981 gccatggcat ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgtcga
     2041 cggcgcggct cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg
     2101 ccgaggaata tcagcagcac aaacgcaatc cagtggttcc attcaagaac aaaccggctg
     2161 gctaacatgc ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata
     2221 aggccggttc gcaacgcttc agaaaatttc ggtttatgga gggtggcacc tttaccgatt
     2281 gatgcagcaa atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc
     2341 atgacaacgt cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat
     2401 acatgaacaa caagccgcga tggttttcgc gttcatgcag gcatagcgat tgatgtgcat
     2461 atggtctcgc ctgactgaaa gtgttcagtc cgtacgtgcc acgttttgca acgagtatgt
     2521 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
     2581 acatattttg agaatataaa acaac
//
LOCUS       DARNVM010000281         2555 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.282, whole genome shotgun sequence.
ACCESSION   DARNVM010000281 DARNVM010000000
VERSION     DARNVM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.282"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1344
                     /locus_tag="SI621_004670"
     CDS             <1..1344
                     /locus_tag="SI621_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4067278.1"
                     /translation="AEPYKYVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDDEITM
                     PGDKVVKFKRASTATYINKSGVFSVAKIDEPRFEKEGLLIEGQRTNYFVKSNTPAEWT
                     STSNIDKTNNGVDEFGFSYAKMRTKDNMTGQSSALSLHTCSASRGIDVSGDNKYCTVS
                     CRVKAPDGLRCRLRFEKYDGSVYTFLGDAYLTFGTLIIEKTGGAANRIAATATKDPVT
                     GWIFYEATIEAVEGETLIGAMIQYAPKKGGITEAGDYIYLATPQFENGGCASSFVITT
                     TAPATRSSDMVTIPTENNIYNRPLTCLVEVNRNWGDIPPNVAPRIFDFSGVPPIESIT
                     YAFNTTEKYYGQLYMQTYKASTSTYVSSVFAGRTDVRKFIGGFNIYSDGTKRVVSNGE
                     ATKTMKTEWTGVKTRTFIRIGGQATSGTRHLFGHLRNLRLWHKELTDAQMGESIK"
     gene            1341..1622
                     /locus_tag="SI621_004671"
     CDS             1341..1622
                     /locus_tag="SI621_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067279.1"
                     /translation="MKDLTLKFADRADFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDSQISSLFDEHAVAVEHQLRSWM"
     gene            1632..2336
                     /locus_tag="SI621_004672"
     CDS             1632..2336
                     /locus_tag="SI621_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEI4067280.1"
                     /translation="MATSTVIPDDIKTLKGDVSKAKEDISSINVKVSTLQTDMDSAKQ
                     DISTRYTKTEVDNKLKNKVEVNDLESGRYGGDFYPLTGREAFYLWGLGTTTAAANLYL
                     NPDPAISSVLRSTSSIRYKHSVETIDSEHADLIFRMHPVWYRSQCENDRRDWGFYGLI
                     AEEVGEIAPQFVHWRPANEDDAPETISSNGLVAEGVMYERLVVPLIHHIQKLTERVDE
                     LESELKLLSVSRSDIG"
     gene            2347..>2555
                     /locus_tag="SI621_004673"
     CDS             2347..>2555
                     /locus_tag="SI621_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067281.1"
                     /translation="MDITPFLHALCAVAAQVLVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPR"
BASE COUNT          751 a          470 c          619 g          715 t
ORIGIN      
        1 gcagagccgt ataaatatgt cttacagcca ctgcctgatg tgtggatacc gtttaacgat
       61 tcactggata tgattacggg cttttcgccg tcatataaaa aaattgttat tggtgatgat
      121 gaaataacga tgcctggcga caaggttgtt aagtttaaac gcgcatcaac tgccacatat
      181 atcaataaat caggcgtatt tagtgttgct aaaattgatg aaccacgatt tgaaaaagaa
      241 ggtttattga ttgaaggaca gcgcactaac tattttgtta aatccaatac tcccgctgaa
      301 tggacgagta ccagcaatat cgataaaact aataatggtg ttgatgaatt tggtttttca
      361 tatgccaaaa tgcgaacaaa agataatatg acaggacaat catctgcact tagtctgcat
      421 acatgcagtg catcccgggg gattgatgtt agtggcgata ataagtattg cactgtttca
      481 tgcagggtta aagctcctga tggtcttcgt tgtcgtttgc gttttgaaaa atacgatggg
      541 tcggtttata catttttagg agatgcttat ttaactttcg gaactctgat aatagaaaaa
      601 actggcggag cagccaatag aatagcagct accgcaacta aagatccggt tacagggtgg
      661 attttctatg aggcaactat agaagctgtt gaaggtgaaa ccttaattgg cgcaatgatt
      721 cagtatgcgc cgaaaaaagg tggtataact gaagcgggag attatattta ccttgcaaca
      781 ccacaatttg aaaacggcgg atgtgcttca tcttttgtta ttacgacaac tgcacccgca
      841 acccgctcca gtgatatggt gacgatccca actgaaaata atatctataa tagaccgctt
      901 acgtgtcttg tcgaggttaa tagaaattgg ggcgatattc ctcctaatgt agcaccgcgt
      961 atttttgatt tttctggtgt gccacctatt gagtcaatta catacgcttt taacacaact
     1021 gagaaatatt acggtcagct ttatatgcaa acttataaag cgtcgacaag tacttacgtt
     1081 tctagtgtgt ttgctggtcg aactgatgtt cgaaaattca ttggtggttt taatatttat
     1141 tctgatggta ctaaacgagt agtttctaac ggtgaggcta ctaaaactat gaaaacggag
     1201 tggacgggcg taaaaacacg gacctttatt cgaattggag gtcaagccac atcgggaact
     1261 cgtcatctat tcggccattt gaggaatctt cgtctctggc ataaagaatt aactgatgcg
     1321 caaatggggg agagtattaa atgaaagatt taacactcaa atttgcagac agggccgact
     1381 tttcggcctt tatggagagc attggctatt atgatgacga gtcgatgcag gatgatattc
     1441 ttattgacgt gataggtaat gtgtacaaag aaaccggaga acttactgaa gatggcgagc
     1501 cggtatgtgt taaggaggac ggatattttg taaatgtgcg catcattaat gattcgcaaa
     1561 tatcgtcatt attcgatgaa cacgcggttg ctgttgagca tcaactccgt agctggatgt
     1621 gaggaagaaa aatggctaca tcgacagtaa ttcctgatga catcaaaacg ctaaagggag
     1681 atgtcagtaa ggcaaaggaa gatatttcct caattaacgt aaaagtatca acgcttcaga
     1741 ctgatatgga cagtgcaaag caggatatca gcaccagata cacaaaaaca gaagtggata
     1801 ataagctgaa aaacaaagtg gaagtgaacg atctggaaag tggtcgttat ggcggagatt
     1861 tttacccgct gactggccgt gaagcgtttt atttatgggg attgggcacg actacagcgg
     1921 cggcaaacct ttatcttaat cctgaccctg caatttcgtc tgtgctgcgg tcaacatcgt
     1981 ctatccgcta taaacattca gtagagacga tagattcaga gcacgccgat ctcattttca
     2041 ggatgcaccc tgtgtggtac aggtcgcaat gcgaaaatga caggcgtgac tggggattct
     2101 atggattgat tgccgaggaa gttggagaaa ttgcccctca gtttgttcac tggcgaccag
     2161 ccaacgaaga tgatgcaccg gaaaccattt ccagcaatgg ccttgttgcc gaaggtgtaa
     2221 tgtacgaacg tctggttgtt ccactgattc accatatcca gaagctgact gaaagagttg
     2281 atgaacttga gtcagaatta aagttgttat ccgtttcccg aagcgatatc ggataaagga
     2341 ggcataatgg atataacacc tttccttcat gcactttgtg ctgtggctgc gcaggtactg
     2401 gttggtcttt ttaccggaaa ctgggcttac ggggcgatag ctggttgtac gttcttcatt
     2461 gcgcgtgaac atacccaggc agaatatcgc tggattgaaa tgttcgggca tggcaagcgt
     2521 atgaatatgc cgtggtgggg cggttttgat ccgcg
//
LOCUS       DARNVM010000282         2446 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.283, whole genome shotgun sequence.
ACCESSION   DARNVM010000282 DARNVM010000000
VERSION     DARNVM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.283"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(197..>2446)
                     /gene="adiA"
                     /locus_tag="SI621_004674"
     CDS             complement(197..>2446)
                     /gene="adiA"
                     /locus_tag="SI621_004674"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEI4067282.1"
                     /translation="ESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDGFAILSS
                     NEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMDRDLLEL
                     VDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAPGHQGGV
                     GFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVFGADRSW
                     SVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSRNRYGII
                     GPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQDLLEKT
                     SDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALSQASYIH
                     VREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEVIDEAVD
                     FRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQDCWVMHP
                     GESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRHGIVPTR
                     TTDFQIMFLFSMGITRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPDTYANMG
                     IHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSIENLPGR
                     IAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEGTEIIDG
                     IYHIMCVKA"
BASE COUNT          561 a          600 c          673 g          612 t
ORIGIN      
        1 atttctcatg tacatatttt tttacaggct aaaaaaagga taaataacag gcaaaaaaaa
       61 gcgcggggtg ggaccgcgca aaaagcatga cattttttgt ttgcagcaat ttttatgaaa
      121 acgttgccat acttaaaagg cctcaagcca ggcggcgtaa tgttatttaa caattacgcc
      181 ttcagcggaa tagtggttac gctttcacac acataatgtg gtaaataccg tcaataattt
      241 ctgtcccttc tgtttcgtgt tcaaacccag ggaaatgatg gtcccaggat tgcagcgagc
      301 gtaaataact tacttgcgga ctgtttttat cgccgaagtt ttcaccagac agcagcatcg
      361 ggattcctgg cggatacggg ataactgagt ttgccgcgat gcgtcctggc agattttcaa
      421 tggataccag ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgt ggggtgattt
      481 ccgccaccgg caggccggaa taggcttcgt tcaaccgtgc gccagggttg ttttctttca
      541 gccaggcaaa catggtgtca cccagatcgt gaatccccat gttcgcgtaa gtgtcaggat
      601 attgttcaac aagttccggc atcacctgcg ccagcggtgt gttggcgtca tagtggcgtt
      661 tgaaggagca aagggtgtta accagagtcc cccatttccc acgggttatg cccatagaga
      721 acaggaacat gatttggaag tcagtggtgc gggtaggcac aatgccgtgg cgaccaagcc
      781 aggcagtgac cagcgccgcc ggaacaccgg tttcttccag ttcaccatct tcacccattc
      841 ccggagcaag gatgctgact ttaattgggt cgagcatact ccagttatcc ggaatatctt
      901 tgaagccgtg ccagctttcg cccggatgca tgacccagca gtcctgaacg gtggtcagca
      961 gtttggttgg tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg gtgacgactt
     1021 ctttgttcca cggtttgaag aaccagctac cgtcagcggt gaactcttta tacagccgcg
     1081 ccatcgcctg acggaaatca accgcttcgt cgatcacttc ctgagtcagt gacaggccgc
     1141 tgttgccgtc catcatcgac accgccacgt cgttggatgc gcagatggca tacagcgggg
     1201 aggtggtggc atgcatcatg taggcctggt tgaagcggga gaagttaatc gccccacgac
     1261 cttcacgtac atgaatataa gaggcctgtg acagcgcatt cagcagtttg tgggtggagt
     1321 gggtggcgaa aacggtaggg ccgttgtgat cgccaggttc gccgcgcatg gcatagtgat
     1381 cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag tgcagacgat
     1441 cggaggtttt ttccagcaga tcctgcgctt ctttagcgtt ataacacacg ccgtcatagg
     1501 tgcagttggt caccacgcag taagacggtt tttgcccggc tttgtcttta gtcagcgggc
     1561 tttcactgat tttcttctgc aaggtttcag gttgcatttc ctgcggatag attggcccga
     1621 taatgccgta gcggttgcgg cttggcacca tgtagaccgg tttcgcgcct gtcagcatca
     1681 agccttgttc gatggattta tggcagttac ggtcaacgac cacgacatcg ttatcggtca
     1741 tgcaagcctg catgatggtg cggttagagc cggaagtacc gacgactacc gaccaggagc
     1801 gatcggcacc aaatacgcgt gcggcatatt tttcgctttc gccaaatgcg ccagtatggt
     1861 caagcaaaga accgagggaa gtgcgttcga tgcccatgtc ggtgcggaac aggttttcac
     1921 catagtagtc atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg ccaccctggt
     1981 ggcctggcgc tgcccaggaa tattcatgga tgtcgctata tttcatcagc gcgctgaaca
     2041 gtggcggcaa cagctgctgg cggtagcggg tcatcgcggc aacggcgcgt ccggcgataa
     2101 agtcggcggt atcttccaga atccaggcga attcatcgac aagctccagc agatcccggt
     2161 ccattgcggc gagggctttt tcccgatcgc ccaacaggaa gaccggcacg ttttgttggc
     2221 gctcatgaag cttaccgatc aattgtctga cgttttgatg ttcgtccgga tgttccattt
     2281 gatagctgaa catcaggcag tcaatggctt cgtttgaaga gagaatggca aaaccatcat
     2341 caaaggaggt ggatttaatc acggtaacat tttgctggct taaagcatct gccagacgct
     2401 caacggcgtt accgacccag gtgtcttgat ggagaaactc gctttc
//
LOCUS       DARNVM010000283         2429 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.284, whole genome shotgun sequence.
ACCESSION   DARNVM010000283 DARNVM010000000
VERSION     DARNVM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.284"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            32..724
                     /locus_tag="SI621_004675"
     CDS             32..724
                     /locus_tag="SI621_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEI4067283.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(773..2272)
                     /gene="pitB"
                     /locus_tag="SI621_004676"
     CDS             complement(773..2272)
                     /gene="pitB"
                     /locus_tag="SI621_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HEI4067284.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          673 a          616 c          573 g          567 t
ORIGIN      
        1 ttaatggata tttaaattaa aggatatatt catgcagtca ataacacctc cattaattgc
       61 cgttattggt agcgatggtt caggcaagtc aacggtgtgt gaacatctca ttaccgttgt
      121 cgaaaaatat ggtgcagccg aaagagttca tttaggaaaa caggccggaa atgtcggtcg
      181 tgcagtgaca aaattaccgt tgatgggaaa atccttacat aaaacaattg aacgaaatca
      241 ggtgaaaaca gcaaaaaaat tgcctggccc tgttccggcg ctggtaatta cagcgtttgt
      301 cgcccgtcgc ttactgcgct ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt
      361 tctaaccgac cgttatcctc aggaccaaat tcctggcgct tacgatggta cagtgttccc
      421 acctaacgtt gaaggcggtc gttttgtctc atggctggca agccaggaac gtaaagcgtt
      481 tcactggatg gcgagccata aacctgatct ggtcatcaaa ctcaatgtcg atctagatgt
      541 ggcctgcgcg cgtaagcctg accacaaaag ggaatcgctg gcaagaaaga tagccataac
      601 gccacagtta acctttggtg gtgcacaact ggttgatatc gacgccaatc agccactgga
      661 aaaggtactg gttgatgcag aaaaagcgat tacggatttt atgaccgcgc gtggttatca
      721 ctagtcaaaa atggaaatgc ccgatcgcca ggaccgggca ttttcaggaa gattaaatca
      781 actgcaatgc tatccagtac aacccaccag aaagaaaaat cgccgccggt aaagtaaaca
      841 cccacgccat caggatgctg gttaccgttt tacgctgtaa cccaccgccg tccaccacca
      901 tcgtccctgc taccgcagac gagaggacgt gtgttgtgga gacgggcatc ccaatataac
      961 tggcaaggcc gatagatacc gccgccgtca tctgcgccgc catcccttgc gcatacgtca
     1021 tgccgcgctt accaatcttc tcaccgatgg tcatcgctac acgacgccag ccaatcatgg
     1081 taccaatgcc gagcgccagt gctaccgcca tgatgatcca caccggagcg tactcaatgg
     1141 tgcttaacat atcgctgcgc aattttttca gcaggttctg atcttcttta ctgacacctg
     1201 gcagtttcgc cagcttcgca gaggtatcag agatgcacag cataatgcgg cgcagctggc
     1261 tgcgctgact cacgcttaac ggctcgtagc tttccatatt gcctggcagc atcgttttaa
     1321 cgcgcgcaat cgcatcaaag gtatttgccg gatgacagtg aaactctgtt acttgcgtgc
     1381 catcagtcga tgtcgcaggc aatggaggtt ccatcgtaat caacttctgc ggcagttcag
     1441 gatgctgttg caggtagtgt tcgaagttgg tgacggcatc gcgggtacgg gtaatttcat
     1501 agccggacgc gttcatattg acgacgaagc cagcaggggc aatccccacc agtaccagca
     1561 tcaccaggcc gatccctttc tgcccgtcgt tagcaccgtg cgaaaacgcc acgcccgcag
     1621 cggaaacaat cagcgcaata cgcgtccaga atggtggctt acgtttgcct tttttctttt
     1681 tgcgatcttc cggaatgcgg tgaatacggt cacgcttttt cgtcccgctc cagtagcggc
     1741 gcagcaggaa tatcaggcct cccgcaatga ccaggccgac gataggggaa acaatcagcg
     1801 aggagaaaat tttggtcact tcacgcaggt ttaacgcatc catcaccgat gagccggtta
     1861 acagcgcgtt ggttaaaccg atgccgataa tcgcaccaat caaggtatgc gaactggagg
     1921 ccggtaaacc aaagaaccac gttcccaggt tccagataat cgccgccagc agcatggaaa
     1981 agaccatcgc caggccgtga gttgacccca tattcagcaa caaatcggtt ggcaacatat
     2041 ggacaatggc ataggcaacg ctaagtccgc ctagcaatac gccaaaaaag ttaaaaaatg
     2101 ccgccatcac cacagcaagt tgtggttgca tggcacgagt ataaataacg gttgccaccg
     2161 cattcgccgt atcatgaaaa ccattgattg cttcgtagaa caacacaaat gccagagcaa
     2221 gtaataacaa aagccccgtg tatatatcaa ggccaacaaa taaatttagc atatattaga
     2281 ttacgccatt ttgaatttac gaacggacgc attatcagtg actttaacgg cgtgggcaaa
     2341 gtgaaatatc attttttaac gataaaaaga gtgttatttc ttgtgtatca gataataagc
     2401 attcattcaa aacctttaga aataatcaa
//
LOCUS       DARNVM010000284         2384 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.285, whole genome shotgun sequence.
ACCESSION   DARNVM010000284 DARNVM010000000
VERSION     DARNVM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.285"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1392
                     /locus_tag="SI621_004677"
     CDS             <1..1392
                     /locus_tag="SI621_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HEI4067285.1"
                     /translation="AVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKKGEHAYDMTLSYK
                     NFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPITMLMRGNVIPALA
                     AAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGSSFSTTLDVAPAEYGKFSFGKGQF
                     TFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESESKF
                     NQINIINHGEDVAQIDTFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSL
                     IAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQKSEP
                     TIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQL
                     NLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMS
                     EEEFMSRAGRFVH"
     gene            complement(1433..2365)
                     /locus_tag="SI621_004678"
     CDS             complement(1433..2365)
                     /locus_tag="SI621_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEI4067286.1"
                     /translation="MANFLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT          743 a          505 c          529 g          607 t
ORIGIN      
        1 gccgtaatct ggggtggtgg cacatggtac acaggtacgc aaattcagcc tggtgtcgaa
       61 aagttcatta aagattttaa cgatgctaaa aagaaaggtg aacatgccta cgatatgacg
      121 ttaagttata aaaactttga caaaggcttt tttaattctc attttcaaat gcaaataaca
      181 tttgataacg gtgcacccga tctcaatatc aagccaggcc agaaagttgc atttgatgtg
      241 gatgttgagc acggtccgtt gcccatcaca atgttaatgc gtggtaatgt catccccgca
      301 ctggcagcgg caaaagtgaa cttagtgaat aatgaactga cacaaccgct atttatcgcc
      361 gcgaaaaata aatcgcccgt ggaagcgaca ttgaggttcg cgtttggtag ctcattctcc
      421 acgacattag atgttgcccc tgcagagtat ggaaagtttt cctttggtaa gggccagttt
      481 acttttaatg gtgatggtag ttcattgtct aacctggata ttgaaggcaa agtcgaagat
      541 attgttctgc aattatcacc aatgaacaaa gtaacggcga aaagctttac cattgattct
      601 ctggcgcgat tagaagaaaa gaaatttccg gttggtgaaa gcgagtcgaa atttaatcag
      661 attaacatta tcaatcacgg ggaagacgtt gcccaaatcg atactttcgt tgcaaaaacc
      721 aggctggatc gcgttaaaga caaagattat atcaatgtca atctgaccta cgaacttgat
      781 aagttaacaa aagggaatca gcaactcggt agtggtgagt ggtcattgat tgctgaatct
      841 attgatcctt cagcggtgcg ccaatttatc atccagtata acattgcgat gcagaagcag
      901 cttgctgcac accctgagtt agcaaacgat gaagttgctc tgcaagaagt gaatgctgca
      961 ttgttcaaag agtatttacc gttattacaa aaaagtgagc cgaccattaa acaaccggta
     1021 agatggaaga acgcactcgg cgaactaaat gccaatctgg atatcagtat tgccgaccca
     1081 gccaaatcct catcatccac aaacaaagat attaaatcgc tcaattttga tgtgaagtta
     1141 ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt tatctgaagg aatggatgct
     1201 gaaaaagcgc aaaagcgggc tgataaacaa atcagcggga tgatgacatt aggtcagatg
     1261 tttcagttaa tcacgattga caacaatacc gcctcgctgc aactgcgtta tacaccgggt
     1321 aaagttgttt ttaacggaca ggaaatgagc gaagaagaat ttatgtctcg tgccggacgt
     1381 tttgttcatt aaaatactca ctcgcctctg ctcattcaga ggcgagtaaa acttacttat
     1441 tctgacgctg tgatgacatc aacgacgtca gaagtcggtc tttttcctgc cacagtgagt
     1501 tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt gatgtagtcg ccatgtaact
     1561 catcggaaat aggctgcaaa tccacatgta cgacaattcg cgtcagttta ccgcttaaca
     1621 tatcgaagaa cggctgacgg ttattgtccg gataacacag cgtaacatta agcagtttat
     1681 cgaattgttt acccagtaca tttagcgcca tcgcaatgcc tgccgccttt ggtggcaaca
     1741 agttttgaaa agtggagtgg gtttgcagat gtttttcttg cgtgaaacgg gagccttcaa
     1801 cgaaattgac aatggtggtg ggatgcaggc gaaacttttc acaagaacgc cgagtggttt
     1861 cgacatcttt accacgtcgc tccggatgac gtaataaata agcgcgggaa taacgcttca
     1921 taaacggcat atccagcgcc cagcacgcca ggccaagaaa aggcacccag gccagctgct
     1981 gcttgaggaa atatttattc atcggaatgt gcttacgaaa cagcacgcac agtacgacaa
     2041 tatctgccca actacggtgg ttacaaataa gcagatacca gttcttctta cttaaccctt
     2101 ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg cagtaatacc gccagacctt
     2161 cgcaccagca atacatcata aaatcacaaa aacgcgaaac ctttcgccaa atgactggca
     2221 caggaagcaa tagttttaca atcccggcaa tgatgatcgg gacggagcaa aaaatagtga
     2281 ccaaaatggt caatacgata ctcagcaaaa gggttatcgc agcgagtatt ctcgtcataa
     2341 tgaatttatt caaaaagtta gccatagaca gtgcgctaaa gaag
//
LOCUS       DARNVM010000285         2361 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.286, whole genome shotgun sequence.
ACCESSION   DARNVM010000285 DARNVM010000000
VERSION     DARNVM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.286"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            47..370
                     /locus_tag="SI621_004679"
     CDS             47..370
                     /locus_tag="SI621_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEI4067287.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            376..1512
                     /locus_tag="SI621_004680"
     CDS             376..1512
                     /locus_tag="SI621_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEI4067288.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQILSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1509..>2361)
                     /locus_tag="SI621_004681"
     CDS             complement(1509..>2361)
                     /locus_tag="SI621_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEI4067289.1"
                     /translation="ADFESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNN
                     FIADYKHEKRGRVTIYAPTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVE
                     FPDDCDVLISYAQPKDESLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILID
                     SMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSD
                     LQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPE
                     QTAESED"
BASE COUNT          584 a          547 c          596 g          634 t
ORIGIN      
        1 tcataatatg cgttaattgt tcttttctat attctctgag ggaaatatgt tcctggatta
       61 ttttgcattg ggagtgctta tttttgtatt tctggtgata ttctatggga ttattatttt
      121 acatgatgtt ccctacctga ttgctaaaaa acgtaatcat ccacatgccg acgccattca
      181 tgttgctggt tgggtaagtc tttttacgtt acatgttatc tggccatttt tgtggatttg
      241 ggccacgctt taccgcccgg agcggggatg gggaatgcaa aaccatgact cctccgttgt
      301 gcaactacaa cagcgtattg ccgggctgga gaaacagctc gccgacatca aatcctcttc
      361 tgccgagtaa tacttatgga tttactgatt attttaacct atgtggcttt cgcatgggca
      421 atgtttaaga tcttcaaaat tcctgtaaat aaatggacca ttcccacagc ggccctgggt
      481 ggcatattta ttgtcagcgg tttaattctg ttaatgaact ataaccatcc gtataccttt
      541 aaagcgcaaa aagcggttat ttctattcct gttgtcccac aggtgacagg cgtggtgatc
      601 gaagtgacgg acaagaaaaa tacgctgatt aaaaaaggtg aggtgctatt tcgactggac
      661 ccgacgcgtt atcaggcgcg ggtggatcgg ctgatggcgg atatcgttac cgcagaacat
      721 aaacagcggg cgttgggcgc agagttagat gagatggcgg cgaatactca gcaggcaaag
      781 gccacgcggg ataaattcgc taaagagtat cagcgttacg cacgcggcag tcaggcgaaa
      841 gtaaacccgt tttcagaacg tgatatcgat gtggcgcggc aaaattatct ggcacaggaa
      901 gcctccgtaa aatcagcggc ggcggaacaa aaacagatcc tgagccagct ggatagcctg
      961 gtgttgggtg aacattctca aatcgccagc ctgaaagcac agctcgcgga agcaaaatat
     1021 aaccttgagc agacgatagt gcgtgcgccg agcgatggtt atgtgaccca ggtactgatt
     1081 cgtccgggta cctatgccgc gtcgctgccg ctacgtccgg tgatggtgtt tatacccgat
     1141 cagaaacgac aaatcgtggc gcagttccgt cagaactcct tgctgcgact ggctcctggc
     1201 gacgatgcgg aagtggtgtt taatgctctg ccaggtaagg tattcagcgg taagctggca
     1261 gccattagtc cagccgttcc cggcggagct tatcagtcga ccggcacctt acagacgtta
     1321 aacacagcgc cgggttcaga tggcgttatc gcgaccattg aactggatga gcatactgat
     1381 ttgagcgcat taccagacgg tatttacgcc caggtggcgg tctactctga tcatttcagc
     1441 catgtctcgg tgatgcgcaa agtactgtta cgcatgacca gctgggtgca ttacctttat
     1501 ctcgatcatt aatcttcgct ttccgccgtt tgttcaggcc gcagttgctg cgactgagca
     1561 agctggtggc gaatttcacg cagcatttca tccagcacat actggacccg ccagggctgg
     1621 tattcccgtt tacggaagat agccaccaga tccagccacc attcatacgg gtgctgaaag
     1681 cagggcacca gcgtgccgtc ttgcaggtcg ctctgcacac ttttgtctgg cgcaaacaca
     1741 atccccaggt gatttcgtgc cagctccagc gcagattgcg tgttgtcgca aacgtaattt
     1801 cccttcacgc gataatcccg cacctcttta ctcccggcga cgttgaagcg ccagatattc
     1861 gcgtcgtcga tcatcatcga gtcgatcaaa atacaggagt gatgttcgag ttcgtcagga
     1921 cggctaatgg gatgtttctc aagatagcgc tggctggcat aagcggttac ggcatattgg
     1981 gtaataaaac tggcaaccag cgattcatct tttggttggg cgtagctgat taaaacatca
     2041 caatcatcgg gaaattcgac gccttcataa aaggcattac gctcaagatt gcaggttttt
     2101 agcgataagg tgatatcacc gatatcttta attttatcga ttacatgttt ggataaatag
     2161 gtaattatgc cggttggtgc atagatagtg acccgaccac gtttctcatg cttataatct
     2221 gcaataaaat tattaagctg ctcgtttctg tccagcatgt cgttgatgta cggtaacagc
     2281 acggtgccaa aaggtgtgag catcagctgt cgggtggttc ggtcaaagac ttttaaaccc
     2341 acttttgatt caaaatcagc a
//
LOCUS       DARNVM010000286         2271 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.288, whole genome shotgun sequence.
ACCESSION   DARNVM010000286 DARNVM010000000
VERSION     DARNVM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.288"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            4..507
                     /locus_tag="SI621_004682"
     CDS             4..507
                     /locus_tag="SI621_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEI4067290.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQRGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(553..969)
                     /gene="rraB"
                     /locus_tag="SI621_004683"
     CDS             complement(553..969)
                     /gene="rraB"
                     /locus_tag="SI621_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEI4067291.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDYVDEDDDGVRH"
     gene            1131..2135
                     /gene="argF"
                     /locus_tag="SI621_004684"
     CDS             1131..2135
                     /gene="argF"
                     /locus_tag="SI621_004684"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEI4067292.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     IAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          578 a          548 c          556 g          589 t
ORIGIN      
        1 actatgaata acattgcgcc gcaatcacct gtaatgcgtc gcctgacgct gcaggataat
       61 cctgctatcg cccgcgtcat tcgtcaggta tccgccgaat acggtcttac cgctgataaa
      121 ggctacaccg tcgccgatcc gaatcttgac gagctgtatc aagtgtacag ccagcgtggc
      181 catgcttatt gggtggttga atacaatggc gaagtggtcg gcggcggcgg gattgcgcca
      241 ttaacaggta gtgaagcgga catttgcgaa ctgcagaaaa tgtactttct tcccgccatt
      301 cgcggtaaag ggctggcaaa aaaactggcc ttaatggcga tggagcaggc acgagaaatg
      361 ggcttcaaac gctgctatct ggaaacgacc gcttttttga aagaagcgat tggtttgtat
      421 gaacatttag gttttcaaca tatcgactat gcacttggct gcacgggcca tgttgattgt
      481 gaagtgcgga tgctacgcga actctgaaaa aatgcccgtt agtgtgtgct atcgggcatt
      541 ttaatcaatt aattagtggc gaaccccgtc atcgtcttca tcgacgtaat cttcatcgtc
      601 gccatcttcg ccattaggat cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa
      661 tttctccgcc agcgtcatca gttgttcaac ctgggcatcg atcagatcgg cattcaatgc
      721 gcactcgctg aggatgtcgc agcaaatcac gatgtcacca tcttcaactt ccagctcttc
      781 tggatcggtc acttcgtaac cgagtttaaa cgcttcaact gccgcttttt ccagagtttc
      841 cagatcgtct gcggaaagat ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc
      901 ttccagtaat tcttcaataa tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg
      961 gtttgccatg gctcattcct cttaatgtcc tgccgatact cttattgtca cacaccactg
     1021 acattgcctc cacctttgtg ataaagattt atgctttaga cttgcaaatg aataatcatc
     1081 catataaatt gaattttaat tcattgaggc gttagccaca ggagggatct atgtccgggt
     1141 tttatcataa gcatttcctg aaattactcg atttcacgcc agctgaactt aacagcctgc
     1201 tgcagttagc cgcgaagctg aaagccgata agaaaagcgg taaagaagaa gccaaactca
     1261 ctggtaaaaa catcgcgctc atcttcgaaa aagactcgac ccgtacccga tgctctttcg
     1321 aagttgccgc atatgaccag ggtgctcgcg ttacttatct cggcccaagc ggcagccaaa
     1381 ttggtcataa agagtcgatt aaagacactg cccgcgtgct tggtcgcatg tatgacggta
     1441 ttcagtatcg cggctatggt caggagattg tcgaaacact ggcggaatac gctggcgtgc
     1501 cggtatggaa cggcctgacc aatgagtttc acccaacgca gctgctggcg gatctcctca
     1561 ccatgcagga gcatttgccc ggcaaagcgt tcaacgaaat gacgctggtc tatgcaggtg
     1621 acgcacgtaa caacatgggc aattcgatgc tcgaagctgc agcgcttacc ggtctggatt
     1681 tgcgtctggt cgcgccacag gcgtgctggc cagaagctgc gctggttacg gaatgccgcg
     1741 ccctggcaca acaaaatggc gggaatatta cgctgacgga agatatagct aaaggagttg
     1801 aaggtgctga ctttatctat accgatgtat gggtgtcgat gggtgaagca aaagaaaaat
     1861 gggctgaacg cattgcattg ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga
     1921 ctggtaaccc tgaggtcaaa ttcctccact gcctgcccgc gtttcatgac gaccaaacga
     1981 cgcttggcaa gaaaatggcg gaagagtttg gcctacatgg cggaatggaa gtgactgatg
     2041 aggtcttcga atctgccgcc agcattgtat ttgatcaggc ggaaaaccgc atgcatacca
     2101 tcaaagcggt gatggtcgcg acgctcagta aataattgat atcgctataa agatgccggg
     2161 aaatacaaaa aatatcccgg catcatatta ttttatgcag tgtgagttct attcttccac
     2221 cccaattttt ctattactgt tcgtaaatct tccggaggat ttaatttttc t
//
LOCUS       DARNVM010000287         2264 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.289, whole genome shotgun sequence.
ACCESSION   DARNVM010000287 DARNVM010000000
VERSION     DARNVM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.289"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..184)
                     /gene="cspI"
                     /locus_tag="SI621_004685"
     CDS             complement(<1..184)
                     /gene="cspI"
                     /locus_tag="SI621_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HEI4067293.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKG"
     gene            complement(547..1044)
                     /locus_tag="SI621_004686"
     CDS             complement(547..1044)
                     /locus_tag="SI621_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HEI4067294.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(1041..1574)
                     /locus_tag="SI621_004687"
     CDS             complement(1041..1574)
                     /locus_tag="SI621_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEI4067295.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRNCRVRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(1571..1882)
                     /locus_tag="SI621_004688"
     CDS             complement(1571..1882)
                     /locus_tag="SI621_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HEI4067296.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(1887..2102)
                     /gene="essD"
                     /locus_tag="SI621_004689"
     CDS             complement(1887..2102)
                     /gene="essD"
                     /locus_tag="SI621_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HEI4067297.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          636 a          540 c          428 g          660 t
ORIGIN      
        1 gacctttagg tccgttctca ataccaaatt caacttcctg attctcagtt aatgttttga
       61 aatcgttgct ctgaattgct gagaaatgga caaacacatc tttgctgcca tctttcggcg
      121 tgatgaaacc aaaacctttt tcagggttaa accatttcac taaaccagtc attttgttag
      181 acataattat taccttttga agaaattagc ccttgggcag aatggtccga aaaaaaatat
      241 cagagagaaa aaccaacaag gaaatctcaa gaggtacaaa taataaaatt ataacaatga
      301 ctgcttcaga taaatttgta acaaaccaga acaccattaa cgcatgatta accacccata
      361 gcaaggatta cttttgtaaa gaaaaacaca gcaatgaaag aatggcttta tttattaata
      421 aaacgcgtca ttctgattaa gaccttttat cttaccctta agatttcaga aattttgact
      481 catggaagag tcctttttat ttaaatttta cattcagcga tgtaaatgtt ccgatttaat
      541 attaccctac atttgatgct ttttatctct taaagattca tagatctgtt gacaagtcac
      601 tcctgcgatg tagcgttcgt cagcaatttc agcataaagc tgagcttctg ctgcaatatc
      661 tccgagcatg ttggtgagca ttccttcggc ggttttggtt gttttgcctc tgacggcagc
      721 ggcaagatct gcggtatgct tcgctgcgtc aaggcgtatg gcatattttt ttgcttcggc
      781 acgcaactgg ttaacactat cagacagata agcagccctg gcagaaattt cagcagattt
      841 ctgttgtgca tctttaacag cctcatcacg ggctatagtt cgcccctgtt caattattcg
      901 agcagcaaat tgagcattta cctcttgtga taatgcggca gcatcacgtt ccgcccattt
      961 tttttgccat cctctgtcgc tccagacatt tccgacgata aatcctgaca acacgagaaa
     1021 aatcaccatg aatatctgat tcactgttct atcccccagc aggttaatgc gctctcctgg
     1081 tcacgacgaa taacctgacc gtaacagtta tttgaacgaa cgcggcaatt gcgtccgcca
     1141 tccttaatcc accagcgaat cgcttcgcat gcacctttac gatcaccagc attcagccgc
     1201 ttataaaacg tcgacgggaa acacttaccg ggaccaatgt tatagggaca aaatgacgcg
     1261 atacccgctt tttgtggttc ggtcagtggt actttaatat tgcgctccac ccatgccagc
     1321 gccttatcac gctcaatggc gttgacctgg tcgcattttt ccttcgacag tttcatattg
     1381 ggaaaaacgg tttttccatc caccactgtg gcaccccgac agatggtcca tatgccagaa
     1441 ccatcgcggt atgccattgt gtggttacct tctttttcgt ccagaaactg gtcaagtatc
     1501 tgaggagcag atgcgccagc accaatcagc gccagaacgg cagccgacag gccgtatctg
     1561 atttttgtgt tcatatatat ttatgatgag gacgctcgtg cttattggca ggattttcaa
     1621 tcttaaagga gtactgatgc tgcagataag actcaacttt ttctgacaat ttttctgcta
     1681 cttccaggaa gacttgccgg acgctccttc tggctgctgc ctcataaaac tccagcgcag
     1741 ctccttcaac acggtccatg gcgacatcca ggtcaaaaat ttcaccgtca aagcgttctt
     1801 tgtcctgtaa ggctacagtt accgtaactt tattctcaaa attacggact cctttcacaa
     1861 ccagttcata gtcttgagtc attggattac tctcctctcg cagccttacg cctgtcttct
     1921 ttaatcttga aataaagatt tgtcagatac gtcagcaggc caaaaaccag gctacccagc
     1981 acaccgattg cagcccactg tgacggagtt actttatcga gtaactgcaa tgcccagaaa
     2041 ccagcattac ccgccgatgt gccataggca acacctgttg ttaacttatc cattgatttc
     2101 atatcctcac cccgatgtac acggatggtg caatatgttt gaaaagatcg gagtctacgg
     2161 ggtagttttg acagcacacg ttgttctcaa cggcgctaaa aaacatacac attaaaaatg
     2221 tgggtaatta ttttgaaaga aaatcatata taaaataata atac
//
LOCUS       DARNVM010000288         2223 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.290, whole genome shotgun sequence.
ACCESSION   DARNVM010000288 DARNVM010000000
VERSION     DARNVM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.290"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..209)
                     /gene="fumD"
                     /locus_tag="SI621_004690"
     CDS             complement(<1..209)
                     /gene="fumD"
                     /locus_tag="SI621_004690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HEI4067298.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            766..2178
                     /gene="pykF"
                     /locus_tag="SI621_004691"
     CDS             766..2178
                     /gene="pykF"
                     /locus_tag="SI621_004691"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HEI4067299.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          601 a          554 c          494 g          574 t
ORIGIN      
        1 cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc actgcgctga
       61 atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg ctttggttcc
      121 tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat ttctcggtac
      181 agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg ccaactatca
      241 gcatatatca atctaaccaa ttagacaaaa atcaaacaaa atcagacaaa taacgcgata
      301 aattatttta actgtagcaa ttgagcgatg atgtatttat acaccggatg aactttcact
      361 tatcttcaca gtgacaaccc cggcaccaga cgttgcgcaa acagcgaagt ttttgcgtaa
      421 ccttttccct ggaacgttaa atctttgata acaatttatt gtcttacaag ttgtatattt
      481 tttgaaacgc tgtttttgtt ttccttttgg attaatttca gcgtataatg cgcgccaatt
      541 gactcttgaa tggtttcagc actttggact gtagaactca acgactcaaa aacaggcact
      601 cacgttgggc tgagacacaa gcacacattc ctctgcacgc tttttcgatg tcacctatcc
      661 ttagagcgag gcaccaccac tttcgtaata ccggattcgc tttccggcag tgcgcccaga
      721 aagcaagttt ctcccatcct tctcaactta aagactaaga ctgtcatgaa aaagaccaaa
      781 attgtttgca ccatcggacc gaaaaccgaa tctgaagaga tgttagctaa aatgctggac
      841 gctggcatga acgttatgcg tctgaacttc tctcatggtg actatgcaga acacggtcag
      901 cgcattcaga atctgcgcaa cgtgatgagc aaaactggta aaaccgccgc tatcctgctt
      961 gataccaaag gtccggaaat ccgcaccatg aaactggaag gcggtaacga cgtttctctg
     1021 aaagcgggtc agacctttac tttcaccact gataaatctg ttatcggcaa cagcgaaatg
     1081 gttgcggtaa cgtatgaagg tttcactact gacctgtctg ttggcaacac cgtactggtt
     1141 gacgatggtc tgatcggtat ggaagttact gccattgaag gtaacaaagt tatctgtaaa
     1201 gtgctgaaca acggcgacct gggtgaaaac aaaggtgtga acctgcctgg cgtttccatt
     1261 gccctgccag cactggctga aaaagacaaa caggacctga tctttggttg cgaacaaggc
     1321 gtagactttg tcgctgcttc ctttattcgt aagcgttctg acgttatcga aatccgtgag
     1381 cacctgaaag cgcacggcgg cgaaaacatc cacatcatct ccaaaatcga aaaccaggaa
     1441 ggcctcaaca acttcgacga aatcctcgaa gcctctgacg gcatcatggt tgcgcgtggc
     1501 gacctgggtg tagaaatccc ggtagaagaa gttatcttcg cccagaagat gatgatcgaa
     1561 aaatgtatcc gtgcacgtaa agtcgttatc actgcgaccc agatgctgga ttccatgatc
     1621 aaaaacccac gcccgactcg cgcagaagcc ggtgacgttg caaacgccat cctcgacggt
     1681 actgacgcag tgatgctgtc tggtgaatcc gcaaaaggta aatacccgct ggaagcggtt
     1741 tctatcatgg cgaccatctg cgaacgtacc gaccgcgtga tgaacagccg tctcgagttc
     1801 aacaatgaca accgtaaact gcgcattacc gaagcggtat gccgtggtgc cgttgaaact
     1861 gctgaaaaac tggatgcccc gctgatcgtg gttgctaccc agggcggtaa atctgctcgc
     1921 gcagtacgta aatacttccc ggatgccacc atcctggcac tgaccaccaa cgaaaaaacg
     1981 gctcatcagt tggtactgag caaaggcgtt gtgccgcagc tggttaaaga gatcacttct
     2041 actgatgatt tctaccgtct gggtaaagaa ctggctctgc agagcggtct ggcacacaaa
     2101 ggtgacgtcg tagttatggt ttctggtgca ctggtaccga gcggcactac taacaccgca
     2161 tctgttcacg tcctgtaata ttgcttttgt gaattaattt gtatatcgaa gcgccctgat
     2221 ggg
//
LOCUS       DARNVM010000289         2194 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.291, whole genome shotgun sequence.
ACCESSION   DARNVM010000289 DARNVM010000000
VERSION     DARNVM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.291"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1052
                     /gene="chaA"
                     /locus_tag="SI621_004692"
     CDS             <1..1052
                     /gene="chaA"
                     /locus_tag="SI621_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HEI4067300.1"
                     /translation="IFPVLALVVLFLWGSSQTLPVVIGINLLALIGILSSAFSVVRHA
                     DVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYSIIMIVTGGLV
                     GFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFSTGQALLVALI
                     SAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSSLWHAIWLIVHLIAVI
                     AVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALKAVLNNQVQRAMNLFFG
                     SVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLCHISFSTGRTNVLNGA
                     AHLALFAAYLMTIFA"
     gene            1457..1564
                     /locus_tag="SI621_004693"
     CDS             1457..1564
                     /locus_tag="SI621_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HEI4067301.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            1992..2099
                     /locus_tag="SI621_004694"
     CDS             1992..2099
                     /locus_tag="SI621_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEI4067302.1"
                     /translation="MTLTQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT          525 a          524 c          561 g          584 t
ORIGIN      
        1 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
       61 cagtggtcat tggcatcaat cttcttgctc ttattggtat tttaagtagc gcctttagtg
      121 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
      181 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
      241 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
      301 ggctggttgg cttttcatta ttgttgggcg gacgcaagtt tgccacccaa tatatgaatc
      361 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
      421 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
      481 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttat
      541 ttgtctacga gcacgaagat gacagtgatg atgacgaccc gcaccacggt aaaccgtctg
      601 cccatagcag cctttggcat gctatctggt tgattgtcca tctgattgcc gttattgcag
      661 tcaccaaaat gaacgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
      721 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
      781 aagcagtgtt gaacaaccag gtccagcgcg cgatgaatct gttctttggt tcagtgttag
      841 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
      901 tgcagtttgc acttggtgcg ccagaaatgg tagtgatggt ggcctcttta gtgctgtgcc
      961 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
     1021 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacagaaat
     1081 cgggggagat aatataaatt cctgcttatt gtatttcttc atactacctg aacaggttgt
     1141 taatcagaat tgcgagcctg taccagaacc tgcacgaaac cactgcaaca gctgttgaaa
     1201 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
     1261 tagtatccac accaagccgg cgttatccgg tgaggcgcaa tgttgcgggg gctttatccc
     1321 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
     1381 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgcaaaa tgttgtcatt
     1441 acaacaaggc ggctatataa cgctcgcgca gtttgccatg attttctggc acgacctggc
     1501 agcaccgatc ctggcgggaa ttattaccgc agcgattgtc agctggtggc gtaaccggaa
     1561 gtaaggtgaa aacgggcgtc aggctgccgt gatggcaata tgcgcccgaa accggccgca
     1621 ggggtaacag cctgcggcct tttttgtctt tatctcttcc acaagtaaat attggcaacc
     1681 cgagctggaa gcatcatcca gagcactcat gaaacgactg taacagctgt tgaaaaaccg
     1741 gaatgcactc ttcattgcat acatttcagg gatgaagtga agatgaattg agtcgtagta
     1801 tccacaccaa gccggcgtta tccggtgagg cgcaatgttg cgggggctct atccctggtg
     1861 gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtataacct gacaacagaa
     1921 cgggggctaa tcttgaacca gaacaattca agaatagccg caaaatgttg tcattacaac
     1981 aaggcggcta tatgacgctc acgcagtttg ccatgacttt ctggcacgac ttggcagcac
     2041 cgatcctggc gggaattatt accgcagcga ttgtcagctg gtggcgtaac cggaagtaag
     2101 gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggagt
     2161 aacagcctgc ggcctttttt gtatccggcc ccat
//
LOCUS       DARNVM010000290         2101 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.292, whole genome shotgun sequence.
ACCESSION   DARNVM010000290 DARNVM010000000
VERSION     DARNVM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.292"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..963
                     /gene="ansB"
                     /locus_tag="SI621_004695"
     CDS             <1..963
                     /gene="ansB"
                     /locus_tag="SI621_004695"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HEI4067303.1"
                     /translation="LATGGTIAGGGDSATKSNYTAGKVGVENLVNAVPQLKDIANVKG
                     EQVVNIGSQDMNDNVWLTLAKKINTDCDKTDGFVITHGTDTMEETAYFLDLTVKCDKP
                     VVMVGAMRPSTSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTT
                     DVATFKSVNYGPLGYIHNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIIYNYANAS
                     DLPAKALVDAGYDGIISAGVGNGNLYKTVFDTLATAAKNGTAVVRSSRVPTGATTQDA
                     EVDDAKYGFVASGTLNPQKARVLLQLALTQTKDPQQIQQIFNQY"
     gene            complement(1476..>2101)
                     /gene="dcuR"
                     /locus_tag="SI621_004696"
     CDS             complement(1476..>2101)
                     /gene="dcuR"
                     /locus_tag="SI621_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HEI4067304.1"
                     /translation="SLGQAKEIISENKKLADLILLDLYINKDNGLDLLPFLHNIHCKS
                     DVIVISAADDSATIKNSLHYGVSDYLIKPFHISRLVDSLTRWKHKKTLLESQKIFNQS
                     ELDLLTHKIIPNTEKIRHLPKGLSTDTLRIICRWIDEHQHKKFTLTELASGIKISRIS
                     CRKYIIWLVSCHILAFDVSCGVTGRPAYRYWIRHQYYPYIKKYSEKP"
BASE COUNT          614 a          462 c          465 g          560 t
ORIGIN      
        1 ttagcaaccg gcgggaccat tgccggtggt ggtgactccg caaccaaatc taactacaca
       61 gcgggtaaag ttggcgtaga aaatctggtt aatgcggtgc cgcaactgaa ggacattgcg
      121 aacgttaaag gcgagcaggt agtgaatatc ggctcccagg acatgaacga taatgtctgg
      181 ctgacactgg cgaaaaaaat taacaccgac tgcgataaaa ccgacggctt cgtcattact
      241 cacggtaccg acacgatgga agaaaccgct tacttcctcg acctgacggt gaaatgcgac
      301 aaaccggtgg tgatggtcgg cgcaatgcgc ccgtccacgt ccatgagcgc agacggtcca
      361 ttcaacctgt ataacgcggt agtgaccgca gctgataaag cctccgctaa tcgtggcgtg
      421 ctggtggtga tgaacgacac cgtactggac ggtcgcgatg tcaccaaaac caacaccacc
      481 gacgtagcga ccttcaagtc tgttaactac ggtcctctgg gttacattca taacggtaag
      541 attgactacc aacgtacccc ggcacgtaag cacactagcg acacgccgtt cgatgtctct
      601 aagctgaatg aactgccgaa agtcggcatt atttataact acgctaacgc atccgatctt
      661 ccggctaaag cactggtaga tgcgggctat gatggcatca tcagcgctgg cgtgggtaac
      721 ggcaacctgt ataaaaccgt atttgacacc cttgcaaccg ctgcgaaaaa cggcactgca
      781 gtagtgcgtt cttcccgcgt accgacgggc gctaccactc aggatgccga agtggatgat
      841 gcgaaatacg gcttcgtcgc ctctggcacg ttgaacccgc aaaaagcgcg cgttctgctg
      901 caactggctc tgacgcaaac caaagatccg cagcagatcc agcagatatt caatcagtac
      961 taatcacctc gccccggtac cgtaccgggg cttttatagt attggtttta gtcagtttca
     1021 gggataatgt gacagacatg aataatgggg accatttcaa tacgttgtgg ttttcttgta
     1081 cccataaaat gtgctgtgtc aaaaactgtg cagtattact tggcagacag acgaacgaat
     1141 tcacagttga atccagagaa ataatatctt ctgttcactg gtacaccaat gttgttccag
     1201 aacaaattca ttcacgataa aatggcggaa aaaacgggca ggattggtaa cgaagcatcc
     1261 gggagaatat actctttctg aacaatgggg gagtgccatg catcagtgta aatgtcaaaa
     1321 aatctgaaat cttccatatt taccgcacat aactattccc tgactatacg tttatcctct
     1381 taatatccgg aacacactct ccagaactta tctcacgatg agtttattgt gattaagtag
     1441 atgtcataat tatggtagat agatgattaa aaggcttacg gtttttcact atatttttta
     1501 atatatgggt aatattgatg tcttatccag taacgataag cagggcggcc agtgacacca
     1561 caggaaacat caaatgccaa tatatggcaa cttaccagcc agatgatata ttttctgcat
     1621 gagattctgg atatcttaat accactggcc agttcagtga gtgtgaattt tttatgttga
     1681 tgttcatcaa tccacctaca aataattcgc aatgtatctg ttgacagtcc tttgggtaaa
     1741 tgtcttattt tttctgtgtt cgggatgatc ttgtgagtta ataaatccag ttctgattga
     1801 ttgaatatct tctgactctc aagaagagtc tttttatgtt tccatcgtgt aagcgaatca
     1861 accagacgtg agatatgaaa tggttttatc agataatctg aaacaccata atgtaatgaa
     1921 tttttaattg tcgcagagtc atcagcagcg gaaattacaa taacatcact tttgcaatga
     1981 atgttatgaa gaaaagggag taaatcaagt ccgttatctt tattgatata aagatcaagc
     2041 aaaatcaagt cagccagttt tttattttca gatataattt ctttcgcctg accaagtgat
     2101 a
//
LOCUS       DARNVM010000291         2077 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.293, whole genome shotgun sequence.
ACCESSION   DARNVM010000291 DARNVM010000000
VERSION     DARNVM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.293"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            67..182
                     /gene="rrf"
                     /locus_tag="SI621_004697"
     rRNA            67..182
                     /gene="rrf"
                     /locus_tag="SI621_004697"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(466..909)
                     /locus_tag="SI621_004698"
     CDS             complement(466..909)
                     /locus_tag="SI621_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEI4067305.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRVELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            1066..1995
                     /gene="metA"
                     /locus_tag="SI621_004699"
     CDS             1066..1995
                     /gene="metA"
                     /locus_tag="SI621_004699"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEI4067306.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          513 a          569 c          488 g          507 t
ORIGIN      
        1 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaacag
       61 aatttgcctg gcggccttag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
      121 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
      181 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc ttttgtttta tctgttgttt
      241 gtcggtgaac gctctcccaa ccaggacaaa tccgccggga gcggatttga acgttgcgaa
      301 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      361 gcagaaggcc atcctgacgg atggcctttt tgcgctggtg aaaaaaaatg ccggatgcga
      421 cgctggcgcg tcttctccag cctgggcagg cacggcaggg gcagcttatt cccccacata
      481 cgccagattc agcaacggat acggtttccc caaatcgtcc acctcagagc gtcccgtaac
      541 cttaaaaccc atcttcttat agaacccaac agcctgctta ttttgctcat taacattggt
      601 tgtcagttcc ggtgccatcg agagcgcatg ttccaccagc atccgaccca cgccgcagcc
      661 gcgcacatca ggatcgataa acagcgcatc catatgctgc ccacttaaca acataaaccc
      721 aaccggccga tcccgctcat taaccgcgac ccacaacggt gcttccggca gaaaggaacg
      781 aaccaactct tccagctcga cccgatactc tgctgataaa aaatcgtggg tggcatcgac
      841 agatcgacac caaatcgcaa cgagttcctc cccttcctca tgccgcgaac ggcgaatact
      901 aataaccatt ttctctcctt ttcgtcattc ttatattcta acgtagcctt ttccttgaaa
      961 ctttctcacc ttcaacatgc aggctcgaca ttggcaaatt ttctggttat cttcagctat
     1021 ctggatgtct aaacgtataa gcgtatgtag tgaggtaatc aggttatgcc gattcgtgtg
     1081 ccggacgagc tacccgccgt caatttcttg cgtgaagaaa acgtttttgt gatgacaact
     1141 tctcgtgcgt ctggtcagga aattcgtcca cttaaggttc tgatcctaaa cctgatgccg
     1201 aagaagattg aaactgaaaa tcagtttctg cgcctgcttt caaactcacc tttgcaggtc
     1261 gatattcagc tgttgcgcat cgattcccgt gaatcgcgca acacgcccgc agagcatctg
     1321 aataacttct actgtaactt tgaagatatt caggaacaaa actttgacgg tctgatagtt
     1381 accggcgcgc cgctgggcct ggtggagttt aatgatgtcg cttactggcc gcagatcaaa
     1441 caggtgctgg agtggtcgaa agatcacgtc acctcgacgc tgtttgtctg ttgggcggtc
     1501 caggccgcgc tcaatatcct ctacggcatt cctaagcaaa cccgcaccga caaactctct
     1561 ggcgtttacg agcatcatat tctccatcct catgcgcttc tgacgcgtgg ctttgatgat
     1621 tcattcctgg caccacattc gcgctatgct gactttccgg cagcgttgat tcgtgattat
     1681 accgatctgg aaattctggc agagacggaa gaaggggatg catatctgtt tgccagcaaa
     1741 gataagcgca ttgcctttgt gacgggccat cccgaatatg atgcgcaaac gctggcgcag
     1801 gaatttttcc gcgatgtgga agccggactg gacccggatg taccgtataa ctattttccg
     1861 cataatgatc cgcaaaatac accgcgagcg agctggcgta gtcacggtaa tttgctgttt
     1921 accaactggc tcaactatta cgtctaccag atcacgccat acgatctacg gcacatgaat
     1981 ccaacgctgg attaatcttc tgtgatagtc gatcgttaag cgattcagca ccttacctca
     2041 ggcaccttcg ggtgcctttt ttatttccga aacgcac
//
LOCUS       DARNVM010000292         2038 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.294, whole genome shotgun sequence.
ACCESSION   DARNVM010000292 DARNVM010000000
VERSION     DARNVM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.294"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1357
                     /gene="chiP"
                     /locus_tag="SI621_004700"
     CDS             <1..1357
                     /gene="chiP"
                     /locus_tag="SI621_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HEI4067307.1"
                     /translation="VTAMSGFMAMPEARAEGFIDDSTLTGGIYYWQRERDRKDVTDGD
                     KYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKA
                     YDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLLAPHWSFMPGTYQGAEAG
                     ANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKVDYLHSIGAKYDFKNNFV
                     LEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRDKVDDRSVNDLYDGTAWL
                     QALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYASSNGRLDIWWDNRSDFNA
                     NGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQSNPDAYYDKNRTIEESAY
                     SLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQDERDVKFMVIAPFTI
                     F"
     gene            1407..1733
                     /locus_tag="SI621_004701"
     CDS             1407..1733
                     /locus_tag="SI621_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067308.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(1818..>2038)
                     /locus_tag="SI621_004702"
     CDS             complement(1818..>2038)
                     /locus_tag="SI621_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEI4067309.1"
                     /translation="KSVFELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGI
                     RLTNHSLYLYGHCAEGDCREDEHAHEGK"
BASE COUNT          529 a          500 c          529 g          480 t
ORIGIN      
        1 tgttacagca atgtcgggct ttatggcaat gccggaggct cgcgccgaag gattcatcga
       61 cgattcaacc ttaaccggcg gtatctatta ctggcagcgt gaacgcgacc gtaaagatgt
      121 taccgacggc gacaaataca aaaccaacct ttctcactcc acctggaacg ccaacctcga
      181 ttttcagtcc ggttatgctg ctgatatgtt cggccttgat attgccgcgt ttacggcgat
      241 tgaaatggcg gaaaacggtg acagctctca cccgaacgaa atcgcgtttt caaaaagtaa
      301 taaagcctat gacgaagact ggtccggcga taaaagcggt ataagcctgt ataaagcagc
      361 ggccaaattt aaatacggtc cggtttgggc gagggcaggt tacattcagc caactggtca
      421 aacgctgtta gcgccgcact ggagctttat gccgggtact tatcaggggg cggaagccgg
      481 ggcgaatttt gattacggcg atgcgggtgc gttgagtttc tcctacatgt ggaccaacga
      541 atacaaagca ccgtggcatc tggaaatgga tgagttttat cagaacgata aaaccaccaa
      601 agttgattat ctgcactcca tcggtgcgaa atacgacttc aaaaataact tcgtactgga
      661 agcagcattt ggtcaggcgg aagggtatat cgatcaatac tttgccaaag ccagctacaa
      721 atttgatatc gccggtagcc cgttaaccac cagctaccag ttctacggta cgcgcgataa
      781 agttgacgat cgcagcgtca acgaccttta cgacggcact gcctggctgc aagcgttgac
      841 ctttggttac cgggcggctg atgtagtgga tttgcgtctg gaaggcacct gggttaaagc
      901 cgacggtcag cagggatact ttctgcaacg tatgactcca acctacgctt cctcgaacgg
      961 tcgcctggat atctggtggg ataaccgttc tgacttcaac gccaacggcg aaaaagcggt
     1021 cttcttcggt gcgatgtatg acttgaaaaa ctggaatctt ccaggcttcg ccatcggcgc
     1081 ttcctacgtt tacgcatggg atgctaaacc cgctacctgg cagagcaatc cggatgcgta
     1141 ctacgacaaa aaccggacta ttgaagagtc tgcctacagc ctggatgcgg tctacaccat
     1201 tcaggacggt cgcgccaaag gcacgatgtt caaactgcac ttcaccgaat acgacaacca
     1261 ctccgacatc ccaagctggg gcggtggtta cggcaacatt ttccaggatg agcgtgacgt
     1321 gaaatttatg gtaatcgcac cattcaccat cttctgatgc ccgacgcggc aggttttgtg
     1381 cctgccgcac gtttgaggaa tttgctatga aaaaactgat tctcatcgcc atgatggcat
     1441 cggggctggt ggcttgtgcg caatcaaccg cgccacagga agacagccgt ctgaaagagg
     1501 catacagcgc ctgtatcaac accgcacaag gttcgccaga aaaaattgaa gcctgccaga
     1561 gcgtgttaaa cgtgctgaag aaagagaaac aacatcagca gtttgccgac caggaaagtg
     1621 tacgcgtact ggattatcag cagtgtctgc gtgcaacgca aaccggaaat gatcaggcgg
     1681 tgaaagccga ttgtgataaa gtttggcagg aaattcgcag taataacaaa taagtgagag
     1741 ctgtaactct cgcttttctt atttcccgtg tataaaaaag ccaacccgca ggttggcttt
     1801 ttctcattca ggctggctta tttgccttcg tgcgcgtgct catcttcgcg gcaatcgcct
     1861 tcggcacagt gaccgtaaag atagagactg tggttagtca ggcgaatgcc atgttttgcg
     1921 gcaatttcac gctgacgcgc ttcgatggag tcatcactaa attcgattac cttgccgcag
     1981 tcgaggcaga tcaggtgatc gtggtgatgt tgctgtgtca gttcaaatac ggatttac
//
LOCUS       DARNVM010000293         1948 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.295, whole genome shotgun sequence.
ACCESSION   DARNVM010000293 DARNVM010000000
VERSION     DARNVM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.295"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            19..1941
                     /gene="yjcS"
                     /locus_tag="SI621_004703"
     CDS             19..1941
                     /gene="yjcS"
                     /locus_tag="SI621_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HEI4067310.1"
                     /translation="MMVNTANAKEEAKAATQYTQQVNQNYAKSLPFSDRQDFDDAQRG
                     FIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETVNPSLWRQSQINGISGLFKVTD
                     KMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAALDLYFQHRPQKPIVAVIYTHS
                     HADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSRRALYSYGLLLP
                     HNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMIIDGLEFDFLMTPGSEAPAEMH
                     FYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPH
                     TWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASR
                     GYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVKALGGSARVINLAQEAN
                     KQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELRE
                     GVHKFSHGTTGSPDTLRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTL
                     NDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMADLVKAKKAKIIGNGAILEEII
                     ACLDNFDLWVNIVTPN"
BASE COUNT          509 a          519 c          502 g          418 t
ORIGIN      
        1 ttaactgccg ccagcagcat gatggtaaat accgctaatg cgaaagagga agcgaaagcc
       61 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
      121 cgtcaagatt ttgacgatgc ccagcgtgga tttatcgccc cgctgctgga tgaaggtatt
      181 ctgcgtgatg cgaacggtaa agtttactac cgcgcagacg attacaaatt tgatattaat
      241 gccgctgcgc cggaaaccgt aaaccccagc ctgtggcgtc agtcgcaaat caacggtatt
      301 tctggcctgt tcaaagtcac cgataaaatg tatcaggtgc gcggccagga tatctctaac
      361 attacgttcg ttgagggcga gaaaggcatt attgttatcg acccgctggt gacgccgcct
      421 gccgcaaaag ccgcacttga tctttacttc cagcatcgtc cgcaaaaacc gattgttgct
      481 gtgatttaca cccacagcca cgccgaccat tatggcggtg tgaaaggcat tatctctgaa
      541 gcggatgtta aatccggcaa agtgcaggtg atcgctccgg caggctttat ggacgaagcc
      601 atcagcgaaa acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt
      661 ctgttactgc cgcacaacgc gcaaggcaac gtgggcaacg gcctgggcgt gacgctggca
      721 acgggcgacc cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg
      781 attatcgacg gcctggaatt tgacttcctg atgactccgg gtagcgaagc gccagccgaa
      841 atgcacttct atattccggc tctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc
      901 ctgcacaact tctacactct gcgcggcgcg aaaacccgcg acaccagcaa gtggaccgag
      961 tatctgaacg aaacgctgga tatgtggggt aatgacgcgg aagtactgtt tatgccgcac
     1021 acctggccgg tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatact
     1081 atcaagtaca ttcacgacca gaccctgcac ctggctaacc agggctacac catgaatgaa
     1141 atcggcgaca tgattaagct gccgcctgcg cttgccaata actgggccag ccgcggctat
     1201 tacggttctg tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac
     1261 ggtaacccgg ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgaaa
     1321 gcgctgggcg gttctgcccg cgtcatcaac ctggcgcaag aagcgaacaa gcaaggtgat
     1381 taccgctggt cggcagaact gctgaaacag gtgattgccg ccaaccctgg tgaccaggtc
     1441 gcgaagaatc tgcaagcgaa taactttgaa cagctgggct atcaggccga gtccgccacc
     1501 tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg aaggggtgca taaattcagc
     1561 cacggcacca ccggttcccc ggacaccctt cgcggaatgt cggtcgaaat gctgttcgac
     1621 tttatgtccg ttcgcctcga tagtgcgaaa gccgcgggta aaaatatcag cctgaacttc
     1681 aatatgagca acggcgataa cctcaacctg acgctgaacg atagcgtgct taactaccgt
     1741 aaaacgctgc aacctgaagc caatgcctct ttctacatca gccgtgaaga tcttcacgcc
     1801 gtgctgaccg gacaagtcaa aatggcggat ctggtaaaag cgaagaaagc caaaattatt
     1861 ggcaatggcg cgatactgga agaaattatt gcctgtctgg ataatttcga tttgtgggtg
     1921 aatatcgtaa ccccaaatta atgccact
//
LOCUS       DARNVM010000294         1942 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.296, whole genome shotgun sequence.
ACCESSION   DARNVM010000294 DARNVM010000000
VERSION     DARNVM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.296"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            235..969
                     /locus_tag="SI621_004704"
     CDS             235..969
                     /locus_tag="SI621_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HEI4067311.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLSGEPVLRLAGLSAATLPHMDYEVAG
                     TPAQRKVWQDKMDQQGAELKARGLLS"
     gene            966..1211
                     /locus_tag="SI621_004705"
     CDS             966..1211
                     /locus_tag="SI621_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HEI4067312.1"
                     /translation="MMRCPFCRHSAHTRTSRYVSDNVKESYLQCQNIYCSATFKTHES
                     ICAVIRSPVTEEKPAPASTAPAVVRKVKGCYSSPFNH"
     gene            1226..1798
                     /locus_tag="SI621_004706"
     CDS             1226..1798
                     /locus_tag="SI621_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HEI4067313.1"
                     /translation="MTTMSLQQAFEVCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQTAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDIFTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
BASE COUNT          499 a          504 c          530 g          409 t
ORIGIN      
        1 cgattgttca ccctttaact tactgtatta cttatctttt ttcttatggt gaacagaggt
       61 gaacagtaaa atataaaaaa acaaacagta agccggtttt tcctgcgacc ttttcctggc
      121 ttgccggtct gaggatgagt ctcctgtgtc agggctggca catctgcaat gcgtcgtgtt
      181 gttgtccggt gtacgtcaca attttcttaa cctgaagtga cgaggagccg gaaaatgtct
      241 gacaacacca tccctgaata tctgcaaccc gcgctggcac aactggaaaa ggccagagcc
      301 gcccatcttg agaacgcccg cctgatggat gagaccgtca cggccattga acgggcagag
      361 caggaaaaaa atgcgctggc gcaggccgac ggaaacgacg ctgacgactg gcgcacggcc
      421 tttcgtgcag ccggtggtgt cctgagcgac gagctgaaac agcgccacat tgagcgcgtg
      481 gcacgccggg agctggtaca ggaatatgac aatctggccg tggtgctgaa ttttgaacgt
      541 gaacgcctga aaggggcgtg tgacagcacg gccaccgcct accggaaggc acatcatcac
      601 ctgctgagtc tgtatgcaga gcatgagctg gaacacgccc tgaatgaaac ctgtgaggcg
      661 cttgtccggg caatgcatct gagcattctg gtacaggaaa atccgctcgc caacaccacc
      721 ggccatcagg gctacgtcgc accggaaaag gctgtcatgc agcaggtgaa atcatcgctg
      781 gaacagaaaa ttaaacagat gcaaatcagc ctcagcggtg agccggttct ccggctggcc
      841 ggactgtcag cggcaacact cccgcacatg gattatgagg tggcaggcac accggcacag
      901 cgcaaggtgt ggcaggacaa aatggaccag cagggagcag agctgaaggc cagaggactg
      961 ctgtcatgat gcgctgccct ttctgtcgtc attcagcgca tacccgcacc agccggtatg
     1021 tgagtgacaa tgtcaaagaa agttatctgc agtgccagaa tatttactgt tcggcgacat
     1081 ttaaaacgca tgagtcaatt tgtgccgtga ttcgttctcc ggtcacggag gaaaaaccag
     1141 caccggcaag cacagcaccg gctgttgtcc gaaaagttaa aggctgttac agctcaccat
     1201 tcaaccatta atcaggagac ggggtgtgac cacaatgtca ttacagcagg cttttgaggt
     1261 ctgccagaat aacaaagcag catggctgca acgcaaaaat gagctggcag cggccgaaca
     1321 ggaatatctg cggcttctgt caggagaagg cagaaacgtc agtcgccttg acgaattacg
     1381 caatattatc gaagtcagaa aatggcaggt gaatcagacc gccggtcgtt atattcgttc
     1441 gcatgaagcc gttcagcaca tcagcatccg tgaccggctg aatgatttta tgcagcagca
     1501 cggcacagca ctggcggcgg cactggcacc ggagctgatg ggctacagtg agctgacggc
     1561 cattgcccga aactgtgcca tacagcgtgc cacagatgcc ctgcgtgaag cccttctttc
     1621 ctggcttgcg aagggggaaa aaattaatta ttccgcacag gatagcgaca tttttacgac
     1681 catcggattc aggcctgacg cggcttcggt ggatgacagc cgtgaaaaat tcacccctgc
     1741 gcagaacatg attttttcgc gtaaaagtgc gcaactggca tcacgtcagt ctgtgtaaaa
     1801 ttccccgaaa atccgcccgt ttttactgga aaaagccatg catcgataag gtgcatggct
     1861 ttgcatgcgt tttcctgcct cattttctgc agatcgcgcc acgcccggcg tggcctgagc
     1921 gtgtcagcgc aactgcatta aa
//
LOCUS       DARNVM010000295         1885 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.297, whole genome shotgun sequence.
ACCESSION   DARNVM010000295 DARNVM010000000
VERSION     DARNVM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.297"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            39..1061
                     /gene="istA"
                     /locus_tag="SI621_004707"
     CDS             39..1061
                     /gene="istA"
                     /locus_tag="SI621_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HEI4067314.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1061..1840
                     /gene="istB"
                     /locus_tag="SI621_004708"
     CDS             1061..1840
                     /gene="istB"
                     /locus_tag="SI621_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HEI4067315.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          490 a          485 c          488 g          422 t
ORIGIN      
        1 tccagggggt aatctgctct cctgattcag gagagtttat ggtcactttt gagacagtta
       61 tggaaattaa aatcctgcac aagcagggaa tgagtagccg ggcgattgcc agagaactgg
      121 ggatctcccg caataccgtt aaacgttatt tgcaggcaaa atctgagccg ccaaaatata
      181 cgccgcgacc tgctgttgct tcactcctgg atgaataccg ggattatatt cgtcaacgca
      241 tcgccgatgc tcatccttac aaaatcccgg caacggtaat cgctcgcgag atcagagacc
      301 agggatatcg tggcggaatg accattctca gggcattcat tcgttctctc tcggttcctc
      361 aggagcagga gcctgccgtt cggttcgaaa ctgaacccgg acgacagatg caggttgact
      421 ggggcactat gcgtaatggc cgctcaccgc ttcacgtgtt cgttgctgtt ctcggataca
      481 gccgaatgct gtacatcgaa ttcactgaca atatgcgtta tgacacgctg gagacctgcc
      541 atcgtaatgc gttccgcttc tttggtggtg tgccgcgcga agtgttgtat gacaatatga
      601 aaactgtggt tctgcaacgt gacgcatatc agaccggtca gcaccggttc catccttcgc
      661 tgtggcagtt cggcaaggag atgggcttct ctccccgact gtgtcgcccc ttcagggcac
      721 agactaaagg taaggtggaa cggatggtgc agtacacccg taacagtttt tacatcccac
      781 taatgactcg cctgcgcccg atggggatca ctgtcgatgt tgaaacagcc aaccgccacg
      841 gtctgcgctg gctgcacgat gtcgctaacc aacgaaagca tgaaacaatc caggcacgtc
      901 cctgcgatcg atggctcgaa gagcagcagt ccatgctggc actgcctccg gagaaaaaag
      961 agtatgacgt gcatcttgat gaaaatctgg tgaacttcga caaacacccc ctgcatcatc
     1021 cactctccat ctacgactca ttctgcagag gagtggcgtg atgatggaac tgcaacatca
     1081 acgactgatg gcgctcgccg ggcagttgca actggaaagc cttataagcg cagcgcctgc
     1141 gctgtcacaa caggcagtag accaggaatg gagttatatg gacttcctgg agcatctgct
     1201 tcatgaagaa aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc
     1261 cttcccggcg gtgaaaacgt tcgaagagta tgacttcaca ttcgccaccg gagcaccgca
     1321 gaagcaactc cagtcgttac gctcactcag cttcatagaa cgtaatgaaa atatcgtgtt
     1381 actggggcca tcaggtgtgg ggaaaaccca tctggcaata gcgatgggct atgaagcagt
     1441 ccgtgcaggt atcaaagttc gcttcacaac agcagcagat ctgttacttc agttatctac
     1501 ggcacaacgt cagggccgtt ataaaacgac gcttcagcgt ggagtaatgg ccccccgcct
     1561 gctcatcatt gatgaaatag gctatctgcc gttcagtcag gaagaagcaa agctgttctt
     1621 ccaggtcatc gctaaacgtt acgaaaagag cgcaatgatc ctgacatcca atctgccgtt
     1681 cgggcagtgg gatcaaacgt tcgccggtga tgcagcactg acctcagcga tgctggaccg
     1741 tatcttacac cactcacatg tcgttcaaat caaaggagaa agctatcgac tcagacagaa
     1801 acgaaaggcc ggggttatag cagaagctaa tcctgagtaa aacggtggat caatattggg
     1861 ccgttggtgg agatataagt ggatc
//
LOCUS       DARNVM010000296         1876 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.298, whole genome shotgun sequence.
ACCESSION   DARNVM010000296 DARNVM010000000
VERSION     DARNVM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.298"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..84
                     /locus_tag="SI621_004709"
     CDS             <1..84
                     /locus_tag="SI621_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEI4067316.1"
                     /translation="RGRSLVQMKSMRHLNSMPAARVLLKKA"
     gene            132..1295
                     /locus_tag="SI621_004710"
     CDS             132..1295
                     /locus_tag="SI621_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HEI4067317.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGDLPH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVIIKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMENALV"
     gene            complement(1342..1683)
                     /gene="symE"
                     /locus_tag="SI621_004711"
     CDS             complement(1342..1683)
                     /gene="symE"
                     /locus_tag="SI621_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HEI4067318.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQKQVREF
                     IGVIAGKQKVA"
BASE COUNT          489 a          399 c          481 g          507 t
ORIGIN      
        1 cgggggagat ccctcgtaca gatgaagtct atgaggcatt taaactccat gcccgcagcc
       61 agagtgttgc tgaaaaaggc gtagatcgcc tggttgaaga tattcacatc tacgcggagt
      121 attactgtgc aatggcattg ggtaaagaaa gtgacaaatc gcttgctacg gcttttcagg
      181 atttgcgcga gttaaaggtc gatgtggcgt atcctttctt actggcgctt tatcatgact
      241 ataaaaatgg cgatttgcct cacgaagatt tcctgagcat aattcgttta attgaatctt
      301 atgttttccg ccgtgcagta tgtgcgattc cgacaaattc tcttaacaag acgtttgcta
      361 ccttttataa ggtcattatt aaagaaaaat atctggaaag tattcaggta cattttctga
      421 atctgccttc atatcgtcgt ttccccaacg atgatgaatt taaacgggaa ttaaaagttc
      481 gcgatctcta taacttccgt agtcgcagct actggttacg acgactggaa aacgataaac
      541 gcagagagcg cgtggaagag tttacgattg agcatattat gccgcagaac gaaaatctgt
      601 cggctaaatg gcgcgaagag ttgggaagcg actggcagcg tgtgcataaa gaactgttac
      661 atacgttggg gaatctcact ttaacgcgct ataactcccg ctacagtgac agacctttcg
      721 cggaaaaacg cgatattgaa gacggcttta agcatagccc gctttatttg aatatcggtc
      781 ttggacagtg cgaaaaatgg gatgaagccg ccattcgcgc ccgtgccgat cgtctggccg
      841 atctcgcggt tcaggtctgg caagcgcctt ctcttcctga agaggtttta gctgtttatc
      901 gggggcagcc ggaaaacaaa accagttaca gcctgagtga ttatcctttt cttgctgatg
      961 gttcgcatag ccgggtgtta tttgatcatc ttcgcgatga agttatgcgc ctggacgcag
     1021 ggatcacgca ggaagtattg aagctctata ttgcgtttaa agctgaaacg aattttgttg
     1081 atgttgtgcc gcaaaaaagc cgactgcgat tgtcgcttaa tatgcagttt catgaactgg
     1141 tcgatccgaa aggtattgcc aaagatgtga caaatgttgg gcgctggggc aatggtgatg
     1201 tggaaattgg tttcagcgac ctcgcacaac ttccttacat tatgggatta attcgtcagg
     1261 catttgaaaa acagatggag aacgcgttgg tataacgtgg cgaataatac ccggtagcac
     1321 tggcgctacc gggtcaatgt atcaggcgac tttctgttta cccgcaatca ccccaataaa
     1381 ttcccgcacc tgcttttgtt tacgcgccga cagcttgcac acctggcgca gcgactgcat
     1441 tagctcgctc tcttcggcgg cgggtggttg ggcggtgagg acaatacagc cttccatcac
     1501 tttgacatct accgccgtgc cggtggcaaa accggcagct tccagccact gacctttcag
     1561 ggtgatggcg ggaatacggc tgtaatccgg gtagcggctc gcataactca cggttaattg
     1621 acggttattt gccggggaga cttctgcttc gaacggttgt gcaatagaat gcgtgtcagt
     1681 catgattact attctcgtga aatagggatt gtgatgagcg gtgtgggtgt gttggcgcac
     1741 atccgcaccg cgctaaatac ctggatatat catcagtaaa tacagggaaa gtccagctaa
     1801 aaattaatga aaataagatg aaaattagcg tgaaatataa tttttggtga tgttaataaa
     1861 gcggctattg agccgc
//
LOCUS       DARNVM010000297         1859 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.299, whole genome shotgun sequence.
ACCESSION   DARNVM010000297 DARNVM010000000
VERSION     DARNVM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.299"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            511..1536
                     /locus_tag="SI621_004712"
                     /pseudo
     CDS             511..1536
                     /locus_tag="SI621_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671526.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1593..1850)
                     /locus_tag="SI621_004713"
     CDS             complement(1593..1850)
                     /locus_tag="SI621_004713"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067319.1"
                     /translation="MFATSILIRTSDEYSIIDLHTNEIINKNVTIGKHVWICDNALVL
                     KGALIGSGSVIGARSVVTGTIPENSLCVGVVNRPVLVLGIF"
BASE COUNT          547 a          404 c          376 g          532 t
ORIGIN      
        1 gtaaaatatc atgtattaac aaccactcta tgtttttatt ctaaaaaaca tttggcaagt
       61 aatgtgtcac ataatactaa gggcttattt taagccctta gtccaatacg gttacatgac
      121 atatatatga aaccatcatt tctttatacc tcaagaaagc ctttagatgt tatttgttgg
      181 cgtcagcatt ttctttgtgg gcagaagcat tgtatacgat cattatcgta tttattgtaa
      241 tgttcaaacg tctaccccac aattatatta cacttatttc gttcattctt cgacatgtac
      301 actctttcat aatttttatg tcatgtagcc atttttccga catcttagtt ctatagaaaa
      361 attatcacaa aatattaaga attttagcta aaccaagtaa aacatgttct gtcagcaatt
      421 aatggtggac ttgtaagtat tccgcataat cgtgccattc atatttagag atcatccggc
      481 attataaatc tgccaacgaa ggagatcgct atgcgtaata tccgttttac tgaacatcag
      541 atcatcgctg tgattaaatc aattgaagcc ggacgaaccg tttaagatgt ctgccgggag
      601 gggaggccgg tatctctgaa gccacctact acaactggaa gtccagatac ggcggcatgg
      661 aagcttctga tattaaaaaa atcaaggatc ttgaggacga gaaccgccgt ctcaaaaaga
      721 tgtttgctga cctgagtctg gagaatcggg cgctgaaaga cattatcgaa aaaaagattt
      781 aaaatcagcc cttaagcgtg agctgccact aatctgataa cgatattcgg gttcagtatc
      841 catcaggcct gccggactct gaacctgagc agaacggttt accatcaccg cccggatact
      901 acgcgtgatg aacccgttat tgccgccctg taggccgcgg ctgagcgata tccgctatac
      961 ggctttccga agcttttcca gattctgcga cggcaaggat actcgtggaa tcacacgtat
     1021 ttactgtctg ctgaagctga attttagccg tagggcaaac aacgactgcc ggtacgcaat
     1081 cccccaccac tggctacatc ggaagcgccg aaccatagct ggtctgtcga ttttatgcat
     1141 gatgctctgg cctgtggccg tcgttttcat acgttcaatg tcgttgatgg ctttaatcgt
     1201 gaggcgttat cgattgaaaa tcgatcttaa tctgccagct ctgcgagtgg tccgtgtact
     1261 tggcaggatc gcggcaaact gcggttatcc agtcatgcag cgcatggaca gtggtctgga
     1321 atttatctct tggcgctggc tgaatggaca gagatatacg tagtaaaact gggagttcat
     1381 ccagccgggt aagccgacgt agaacgcttt catcgagcgt tctaacagga tgtaccgtac
     1441 agaaatactc gatttttatt tattcagaac gctgaatgaa acgcgggaaa tcacggagaa
     1501 aaggttatca gaatataact gtgaacgcag catgaataac tgaacaatat gacaccagag
     1561 gaatatcgac agcaccttat ttgggcggga tctcaaaaaa tgcctagaac taaaacgggt
     1621 ctatttacaa caccaacaca taagctattc tcaggtatag taccagtcac aacactcctt
     1681 gcacctataa ctgaaccact tccaattaat gcccccttca aaaccagagc gttatcacat
     1741 atccagacgt gttttccaat tgtaacattt ttattaatta tttcgttagt atgcaaatct
     1801 attattgagt actcatcaga cgttcttatt agaattgatg ttgcaaacat acaatcatc
//
LOCUS       DARNVM010000298         1830 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.300, whole genome shotgun sequence.
ACCESSION   DARNVM010000298 DARNVM010000000
VERSION     DARNVM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.300"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1830
                     /locus_tag="SI621_004714"
     CDS             <1..>1830
                     /locus_tag="SI621_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEI4067320.1"
                     /translation="KNDYKISLFPDTTYRELNKAAQRSITAPDRIGEGKTWVYQRDTM
                     VERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPTG
                     QQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRKNI
                     SEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIA
                     LVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQV
                     LIPFIIDPVTKERDQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTT
                     PKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNL
                     FNPKYQKKAVMNYMEYLVTCPLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDL
                     QALASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPF
                     SRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIW
                     SAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG"
BASE COUNT          632 a          359 c          350 g          489 t
ORIGIN      
        1 taaaaaacga ttataaaatt tccctttttc cagatacaac ttaccgagag ttgaataaag
       61 cagcacagcg tagcattaca gcgccagaca ggataggaga aggaaaaaca tgggtttatc
      121 aacgagatac aatggttgaa agaggtgata acagcggtgt tcatcagtat ggtccagctg
      181 aacattttac tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg
      241 ctattaacat tcctgactat gaactggcag ctgatgtata taatattaac gtaacgtcac
      301 ctaccggcca gcaagaaaca tttaaaatat taatcaatcc agaacatcta cggcaaacac
      361 ttgagcgtaa atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa
      421 aacctggcga agcgattctt catgctttta atgccaccta tcaacaaatc aggaaaaata
      481 tatctgagtt tgcacgttgt cattatgggt atatacaaat ccctcccgta accactttcc
      541 gcgccgacgg accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac
      601 ccgaagatct ttgtactatc cataatccaa tgggagattt gcaggatttt atcgcattgg
      661 ttaaagatgc caaaaaattt ggtatcgata tcatccctga ttatacattt aactttatgg
      721 gaatcggggg tagtggtaaa aatgatctgg attatccctc tgccgatata cgagcgaaga
      781 tcagtaaaga tatagaaagt ggtatccctg gctattggca aggtcaggtc ttgattccct
      841 ttattataga tccagtaaca aaagaacgtg accaaatcca tccagaagat atacatctca
      901 ctgcaaaaga cttcgaagca agtaaagata atatttctaa ggatgaatgg gaaaacctcc
      961 atgcattaaa agaaaagcgt ttaaatggaa tgcctaaaac aacacccaaa agtgaccagg
     1021 ttattatgtt gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac
     1081 gttatgatgc agcaaaacac tcaaaacatg aacaaataga gaggtcgata accccaccgc
     1141 ttaaaaatta taatgagcgg ttgcacaata caaatttatt taatccaaaa tatcaaaaaa
     1201 aagctgttat gaattacatg gaatacctgg taacttgtcc gttagatgaa caacaaatgt
     1261 catcgctgct ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga
     1321 cgataaaagc cttttcattc ggtggagatc tccaggccct tgcatcaaaa ccgggctcca
     1381 caatctcaag catcccatca gaaagacgga tattgattaa catcaaccac gatttcccta
     1441 acaatggtaa tcttttcaat gactttttat ttaaccatca gcaagatgaa caattagcaa
     1501 tggcatatat ggccgctctc ccgttcagca ggcctttagt ttactgggat ggtcaggtat
     1561 taaaatcaac gactgagatt aaaaatgatg atgggtctac tcgtgtcggc ggtgaggcgt
     1621 ggcttaataa aggttgctct acctatcagc agctctacaa tgaattccac gcattatata
     1681 tagataaagc aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg
     1741 cctttagtcg tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa
     1801 taaataaagg aaacgaagaa gttgaaggta
//
LOCUS       DARNVM010000299         1783 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.301, whole genome shotgun sequence.
ACCESSION   DARNVM010000299 DARNVM010000000
VERSION     DARNVM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.301"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..312)
                     /locus_tag="SI621_004715"
     CDS             complement(<1..312)
                     /locus_tag="SI621_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BapA prefix-like domain-containing protein"
                     /protein_id="HEI4067321.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVRDGNDLLIYMQDGTVIRCNGYFLQAANTSEQSELVFVDGQQLTHVTFADTAAGGL
                     AP"
     gene            complement(421..1776)
                     /locus_tag="SI621_004716"
     CDS             complement(421..1776)
                     /locus_tag="SI621_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEI4067322.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDIKLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHRTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT          408 a          464 c          431 g          480 t
ORIGIN      
        1 gggcgctaat ccacctgccg cggtatcggc aaatgttacg tgggtcagtt gttgaccatc
       61 gacaaacacc agttcggatt gttcgctggt atttgccgct tgcaggaaat aaccgttgca
      121 gcggatcacc gtgccgtcct gcatatagat caacaagtca tttccgtcac gaacataacg
      181 cgcaacggca gatgcctgag catgaacctg caccacagag ggctcataaa tgacaaccgt
      241 aacgtcgcca ggattgatca gcgtttgttt gacggatgtt tttcgcgaaa taacatcaat
      301 aatcaggctc ataatggcat tcctgataaa tggcgcacat ccttagcgag tccatttatc
      361 taaataagac aaaggtaatc actcaacaac gatgcatacc tgatattaat aatggcagga
      421 ttatttcaag tcgggtaagt cgttgtataa gccctgttta attcccgcta atggaataag
      481 gttattaacc gcagcggcat aattcactgc ggcgacccag ccatcataat tggcatttat
      541 ttcagccgat tgcgcctgaa agacatcttg ctcgacggtt aatagatcat tcaggctgcg
      601 tttacccaat ttatattcat tttggtagac atctcgcgtt ctgtgcgcac tttcagattg
      661 cgctaaccca gcctcttcac gaccccgtgc gcctgtccag ttggcatatg ccacagacgc
      721 tcgttgcagc acatccagtt ttgcctgttc gacctgcgag gcagagattt tttgttgccc
      781 ttcagcctgt tgcacctggg cagaaaccgc gccgccctga tacagcggcg cgttaacgtt
      841 cagttgtagc tgatcatccc aatacgagcg gtcgctggtc tggtagcgcg ttttgccccc
      901 ctgaatactc agcgttggcc agtattgcgc tttcgttttt tccacgccgt actgtgctga
      961 ctggcgtaag ttttctgccg ccagcaccag cgggatggac tggtaatcaa tatttttcag
     1021 cgataccggc tgctcggcta attcagcagg tggcgcggct atcgcctccg gctgtacgcc
     1081 agtgagcacc gccagttgcg ctttggcgct tgccatctgc gcctgatatt gctccagcgt
     1141 tgagcgcatt ccggcaatgc gcgtctgggc ctgtaattca tccgacgacg agttcaggcc
     1201 agcgttagca cgcaatgctg ccatgttgta gacgttttcc agcgagtgaa tattgcgttg
     1261 agccgcatcg cacaaagcct gataacgact gacttccata taggcagtcg ccgttttctc
     1321 tgcaacatcg gttaaggttg ccatcaattt aaagcggtag ctgtcacggg cggcagtttg
     1381 cagtttgata tcgttgttag ttttaccaaa gtcatacacc agctgtgtca gggttatgcc
     1441 atacgaaacg ttgttatcaa gcctgccact ggagtccgtt tgtcgggacg gtcccgcatt
     1501 gccggttaat ccaacgtgcg gataccaggc acttttggct tcatcaattt gcgcttcgcc
     1561 aatacctatc tgcgccgcct gttgagtgac gtcgggattt cgcgcaaagg cgcgtaatat
     1621 actttcctgt aaagataatt gggcaactgg cgttaccgct ggggcgttcg cccattgtgg
     1681 cagcggtgca gcaattgtag cttctcccat caccataaaa cttattaaaa aaataaaccc
     1741 attattttta cggcttttaa actggaatcg cgccatagat aag
//
LOCUS       DARNVM010000300         1757 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.302, whole genome shotgun sequence.
ACCESSION   DARNVM010000300 DARNVM010000000
VERSION     DARNVM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.302"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(92..634)
                     /locus_tag="SI621_004717"
     CDS             complement(92..634)
                     /locus_tag="SI621_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HEI4067323.1"
                     /translation="MIYPTNTGKSGEHLRLATLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKTA"
     gene            complement(631..921)
                     /locus_tag="SI621_004718"
     CDS             complement(631..921)
                     /locus_tag="SI621_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HEI4067324.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIAGSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(921..1520)
                     /locus_tag="SI621_004719"
     CDS             complement(921..1520)
                     /locus_tag="SI621_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HEI4067325.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSHGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSLEQVENVAAQLLEFA"
BASE COUNT          455 a          461 c          363 g          478 t
ORIGIN      
        1 cgggttctat taaagtttaa ttgcgcctga ttcgccacgc gatacagctt tgcgaagcgt
       61 agcaaaattg aagcagttta tgcgtaaaaa atcaagccgt tttttgagcg aatgattctc
      121 gcatgggaat gtatagcgca tactcagcaa cggccaacca attagcaatt cgcttttcgc
      181 atgtgctaaa acaccactct gggtgtgcat catttagcaa ttcagccatt ttgcgtttgg
      241 tcatcccccg cccctcatac cgttgccgaa ggacgctaat caatcctgga tgctctgcca
      301 gcacctcact tatgactcga tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      361 agctcttttg cttgccgttg atcatctctc tcaaaaacgc ttccagctca gctttctcta
      421 ttcccgcttt tttcattctg cgcagggctt cattgatggc tgttttcgtc aattttttgg
      481 atgccaacaa ctgattgaac atatttcctg acctgccacc gccaatatac gaccagcgcc
      541 cccacatacg cagtttgccc tgaatccaga cactttccag cgtggcgaga cgaaggtgtt
      601 ctccgctttt tcctgtattc gttgggtaaa tcacaaatat ccctcctttc tccagatttc
      661 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      721 ggtttttacc cgcccgtcga ttagatcgtg gcatgagcta ccggcaatcg ccgcctgcat
      781 atcgtgtggt tttgtcgctg ttccgcacgt tcccgccagt cggtaatgcg ccagcacaga
      841 cgtttccgga tcgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
      901 cgctttacgt aaatccacca ttacgcaaac tccagcagct gcgcggccac attttcgact
      961 tgttccagag aggaaaattt acggaacaga atccagttcc acagcacatt cagaacagat
     1021 ttataaacct gctgaaactc ggtttcgtcc atattcgcaa aagctatgga tttcgcccga
     1081 cgcccatggc taccgtccgg ataaaaatgt tcggtgtaaa atccagcctg aatggttacc
     1141 cactcgcgaa aagcctcaaa cgactttagc aatgccgtat cccgggttct gcgtgtcgca
     1201 actgtattca gatattgctc tgcggcatca ctcagggctg gcgtgtgttc ccgaccaact
     1261 gattcgcaca ggtaatcaac gaaaccagac agcagttctc gttcgcgagg cgtgatcgcc
     1321 ccaccgaccg gagtccagta atcgaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1381 aatgcgtagt tacgcacacg cttaaaatca gcgtgtatcc actcgcctat tttaatttga
     1441 tgcagaaaat cacaactctc cggcgtcgcc gggagaagta aaccagaaga agtttgtttg
     1501 accagttgta tatgcgccat ttctcaatct ctctatggcg cagtgcagca gatgccagtt
     1561 gttcaggctg acgaataaag tataaataaa ctggctatgg tgtaaagccc cacatagcat
     1621 gaacaaacac tacatatcaa atagctggta caaggataga aatacgacac ttattattaa
     1681 aaacgattag ctaaattaca ttttaatgtt atgaaaaagt tcttttttat cataacattt
     1741 caataaaagc attacag
//
LOCUS       DARNVM010000301         1709 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.303, whole genome shotgun sequence.
ACCESSION   DARNVM010000301 DARNVM010000000
VERSION     DARNVM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.303"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1709
                     /gene="rhsB"
                     /locus_tag="SI621_004720"
     CDS             <1..>1709
                     /gene="rhsB"
                     /locus_tag="SI621_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HEI4067326.1"
                     /translation="LGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSR
                     SESLWLVRGGVLKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVP
                     EADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTT
                     QAQRAEEARQQASSGGTEPSAFPDTLPDYTEYGRDNGIRLSAVWLTHDPEYPENLPAA
                     PLVRYGWTPRGELAAVYDRSGKQVRSFTYDDKYRGRMVAHHRAGRPEIRYRYDSDGRV
                     TEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGGLKRVVKKEHADGSVTQSQF
                     DAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGL
                     ELRREYDESGRLIQETAHNGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLT
                     VTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRTYDSRGQLIAVKDTQGHETRYEYNAA
                     GDLTAVIAPDGSRNGTQYDAWGKPVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFR
                     YDVL"
BASE COUNT          370 a          499 c          554 g          286 t
ORIGIN      
        1 ctcggccccg gctggaaaat gcctgcggat atccgcctac agctgcgcga taacacactg
       61 atactcagtg ataacggcgg caggagcctg tattttgagc acctgtttcc cggtgaggac
      121 ggttacagcc gcagcgagtc actctggctg gtgcgtggcg gtgtcctgaa actggatgaa
      181 ggtcaccggc tggccgcact ctggcaggcg ctgccggaag aactccgctt aagtccgcat
      241 cgttatctgg cgacaaacag tccgcagggg ccgtggtggc tgctcggctg gtgtgagcgg
      301 gtgccggaag cggatgaggt gctgcccgcg ccgctgccgc cgtaccgggt actgaccggg
      361 ctggtggacc gcttcgggcg cacacagacg tttcaccgtg aggctggcgg tgaattcagc
      421 ggcgaaatca ccggcgtgac ggatggtgcc gggcgtcact tccggctggt actgaccacg
      481 caggcgcagc gggcagaaga ggcccggcag caggcctctt ccggcgggac ggaaccgtcc
      541 gcttttcctg ataccctgcc ggactacacg gaatatggcc gggacaacgg tatccgtctg
      601 tctgccgtgt ggctgacaca cgacccggaa tacccggaga atttacctgc cgcgccgctg
      661 gtgcgctatg gctggacgcc acgcggcgaa ctggcggcgg tgtatgaccg cagtggcaaa
      721 caggtgcgca gctttactta cgatgataaa taccggggcc ggatggtggc gcaccaccgc
      781 gcgggccggc cggaaatccg ttaccgttac gacagcgacg ggcgggtgac agagcagcta
      841 aacccggcag gcttaagcta cacgtatcag tatgagaaag accgcatcac catcaccgac
      901 agcctggacc gccgtgaagt gctgcacacg cagggcgaag gcgggctgaa gcgggtggtg
      961 aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt ttgacgcggt gggcaggctt
     1021 aaggcacaga cggatgccgc aggccggacg actgagtaca gcccggatgt ggtgacggga
     1081 ctcatcacgc gcatcaccac accggatggc agggcatcgg cattttacta taaccaccac
     1141 agccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga atatgatgaa
     1201 tccggccgtc tgattcagga aaccgcacac aacggcgata tcacccgcta ccgttatgat
     1261 aatccacaca gtgacttacc ctgcgcaacg gaggatgcca ccggcagccg gaaaaccatg
     1321 acatggagcc gttacggtca gttgctgacc gtcactgact gttccggtta tgtaacccgt
     1381 tatgaccatg accgttttgg tcagatgacg gcggtgcacc gcgaggaagg gctgagtcag
     1441 taccgcacat acgacagccg tggacagtta attgcggtga aagacacgca gggccatgaa
     1501 acgcggtatg aatacaacgc cgccggtgac ctgaccgccg tcattgcccc ggacggcagc
     1561 agaaacggga cacagtacga tgcgtgggga aagcccgttc gtaccacgca gggcggtctg
     1621 acgcgcagta tggaatacga tgctgccggg cgggtcatcc gcctgaccag tgaaaacggc
     1681 agccacacca ccttccgtta cgatgtact
//
LOCUS       DARNVM010000302         1655 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.304, whole genome shotgun sequence.
ACCESSION   DARNVM010000302 DARNVM010000000
VERSION     DARNVM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.304"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..142
                     /locus_tag="SI621_004721"
     CDS             <1..142
                     /locus_tag="SI621_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HEI4067327.1"
                     /translation="NWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLR
                     PS"
     gene            249..530
                     /locus_tag="SI621_004722"
     CDS             249..530
                     /locus_tag="SI621_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEI4067328.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            496..1572
                     /locus_tag="SI621_004723"
     CDS             496..1572
                     /locus_tag="SI621_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HEI4067329.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     IAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRGN
                     QLETIKEKLGGILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDFLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT          577 a          324 c          360 g          394 t
ORIGIN      
        1 taactggaca agatattcaa aatctaaact aactaaatca gaatgcgaaa aaatgctctc
       61 ggggaaaaaa gaagcaggcg tttccagaga gcagaaagta aagctgataa attttaattg
      121 cgagaaactt cggccctcgt gagttgcatt atatccaaat tataacttca tagctgatta
      181 ttaaaaatca atcacgtccg ccagtattct gtatatttac tggcggtcat atcgtaagag
      241 gtatggcaat gaatcttgtg acactcaaaa cgtggggaaa actcagatat ccggataacc
      301 caccatcaat atcaacgctg agacgatggg caaggaatgg aaacatttat cctgcacctg
      361 aactacacgg gaggagttac agggtggttc cggaggcttt ctatatcaac ccaaataagg
      421 ttgataccga tataacacac catcagccta atgggcgaca agggagagac agtccgttac
      481 tggagaagtt aaaacatgca gcggaaaaaa tacgatccca atttgcctag gaacttaaca
      541 tatcgaagga gggacaaagc atattactgg cgcaaccctc ttacgaaaga agaatttaca
      601 ctaggtaaaa tttcaagaag agatgcaatc gcgcaggcaa ttgaagcaaa tcattatata
      661 tacaaaaact actctcctgc tgccttaatt gaaaaactta aagggttcga ctcatttact
      721 atggcagact ggattgaacg ttacaaaacg attcttataa ggagaaaagt gtccagaaat
      781 acttataaaa ttcggggaaa tcaactggaa acaataaaag aaaaattagg agggatctta
      841 ctgacagaaa taaccactcg ccatattgcc gagtttcttg atttgtggat tgaaggaggg
      901 aaaaacacga tggcaggatc aatgcgttct gtgttatctg atatgttccg cgaagccatt
      961 gttgaagggc gtatatctca aaatccagta acgccaacaa gagcaccgaa aatagtagtt
     1021 acaagagaac ggctgaaact aaagacatac aactgcatca gggaggcagc agatcaactt
     1081 ccggcatggt tcccattagc tatggactta gcccttgtaa caggacaacg tcgcgaagac
     1141 ataacgaata tgcggttcag tgatatttat gatgatcgtc ttcacatcag gcaaattaag
     1201 acaggaatga tgattgctat ccccctgtca ctcagccttc cggtcgctgg tttacgtctt
     1261 ggtacagtag ttgaacggtg ccgcctggta agccggggag attttctaat cagtgccggg
     1321 attagaaaaa acagccctga cggcagcatt cacccggacg gcctgacaaa aaaatttgtc
     1381 gcagccagaa aattaacagg tatccagttc agtgaaaacc caccaacttt tcacgagatc
     1441 agaagcctgg ctggacgatt atacaaagaa acatgtggag aagaatttgc tcagcgtcta
     1501 cttggccaca catcggagaa gacaacaaaa atgtatcttg atgagagaga aaaaacgtac
     1561 ttactgctct gattttaacg taaatggatt gttaaatgta ttttggttgt gatataacca
     1621 aaaaagaccg gaatacagaa attcgagtaa atttc
//
LOCUS       DARNVM010000303         1641 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.305, whole genome shotgun sequence.
ACCESSION   DARNVM010000303 DARNVM010000000
VERSION     DARNVM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.305"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            74..>1641
                     /locus_tag="SI621_004724"
     CDS             74..>1641
                     /locus_tag="SI621_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEI4067330.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECTPLVEQNLMPWLATGAAALITTLAVVELNDDDDHHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDVPDDTPTPPKPISFNNDVILDKTEKTLTIRDS
                     VFTYTENADGTISLQDNSGRKATINLWQIDEANNTVALEGVSADGTTKWRYNHNGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVD
                     NKGTMTVTDPESIGIQIDGDKAVVNNEGDSTISNGGTGTQINDDDATANNNGKTTVDG
                     KDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMGIQID
                     GDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGKVIQG
                     GDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQIDGDKAVVNNEGDSSISNGG
                     TGTQINGDDATANN"
BASE COUNT          487 a          404 c          431 g          319 t
ORIGIN      
        1 ttcaatgttt taaataacta attggtcggg ttagtgcatc cggctttctt tatattcgcc
       61 agaaggattt attatgcaaa ggaaaacttt attgtcggcc tgtattgcat tagctctgag
      121 tggtcagggt tgggcggcag atatcacaga ggtagaaacc accacaggtg aaaagaaaaa
      181 tactaatgtg acttgtccgg cagatccagg aaaactcagt ccggaagagc ttaaacgctt
      241 accctctgaa tgcacccctt tagtcgaaca aaacctgatg ccatggcttg ccacaggcgc
      301 tgctgcgtta atcacgacct tagccgtagt ggaactaaac gacgatgatg atcatcatca
      361 tcgcaacaat tctccactcc caccgacgcc ccctgatgat gattcagacg acactccagt
      421 gcccccaact cctggcggag atgagataat accggacgtt ccggatgata cgcctacacc
      481 tcccaaaccg atttcgttta ataatgacgt tattctcgat aaaacagaaa aaacgttaac
      541 tattcgcgat tcagttttta cttataccga gaatgctgac gggactattt ctctgcaaga
      601 taacagtggt cgtaaggcaa cgattaatct ttggcagatt gatgaagcga ataatactgt
      661 tgcccttgaa ggtgtgagcg cagatggcac aacaaagtgg cgatataatc acaacggtga
      721 gcttgttata acgggtgata atgccacagt aaacaacaat ggcaaaacca ccgttgacgg
      781 taaagactcc accggcacgg aaatcaacgg taataacgga aaagttatcc aggacggcga
      841 tctggatgtc agcggcggcg gtcacggcat tgatatcacc ggcgacagcg cgacggtgga
      901 taacaagggc accatgaccg tcaccgatcc ggagtccatc ggtattcaga tcgacggcga
      961 caaggcggtt gttaacaacg aaggcgacag caccatcagc aatggtggca ccggcacaca
     1021 gattaacgat gatgatgcca cggcgaataa caacggcaaa accaccgttg acggcaagga
     1081 ttccaccggt acagaaatca acggtaataa cgggaaggtt atccaggatg gcgatctgga
     1141 tgtcagcggt ggcggtcacg ggattgatat taccggcgac agcgcaacgg tggacaacaa
     1201 gggcaccatg accgtcaccg atccggagtc catggggatc cagatcgacg gcgacaaggc
     1261 catcgtcaat aacgaaggcg agagcaccat caccaacggc ggcaccggta cgcagattaa
     1321 cggtgatgac gccacggcga acaacaacgg caaaactacc gttgacggta aagattccac
     1381 cggtacagaa atcaacggta ataacggaaa agtgattcag ggcggcgatc tggatgtcag
     1441 cggcgggggc cacggcattg atatcaccgg tgacagcgcg acggtggata acaagggcac
     1501 catgaccgtt accgatccgg agtccatcgg tatccagatc gacggcgaca aggcggttgt
     1561 caataacgaa ggcgacagtt ccatcagcaa cggcggcacc ggtacgcaga ttaacggaga
     1621 tgacgccacg gcgaacaaca g
//
LOCUS       DARNVM010000304         1608 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.306, whole genome shotgun sequence.
ACCESSION   DARNVM010000304 DARNVM010000000
VERSION     DARNVM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.306"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            185..916
                     /gene="bluR"
                     /locus_tag="SI621_004725"
     CDS             185..916
                     /gene="bluR"
                     /locus_tag="SI621_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HEI4067331.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIEAPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1137..1541
                     /locus_tag="SI621_004726"
     CDS             1137..1541
                     /locus_tag="SI621_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HEI4067332.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHYRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          489 a          331 c          349 g          439 t
ORIGIN      
        1 atgatttcag taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tccacattaa
       61 aaataatgtt tcattcaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta
      121 tattgtacaa agtattattg ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg
      181 aattgtggct tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac
      241 tctccgggcc tggcaacgcc gctacggttt gttaaaacca cagcgcagtg aaggcggaca
      301 ccgactcttt gatgaagaag acatacaacg catcgaagag atcaagcgtt ggataagtaa
      361 tggcgtccct gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga
      421 tgactggagc cgcctgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa
      481 actacgcgcg agaattattt cactgggtcg agagtaccca gtagatcaat tgattaatca
      541 tgtttacctt cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag
      601 cagtatgttt gacggcgcat taattgaata cgcagcagcc tcgcttttcg aaatgcgccg
      661 taagcccggt aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg
      721 gctggaagca tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat
      781 tgaagcgccg cgcccggaac tgttcccaac gcaaacattg attgtctgga caggcatggc
      841 accaacgaga aggcaaaacg aacttttaca acactggggt gagcaaggtt ataaagtcat
      901 ctttcatgcc ccctaatttc acctttatcc cgccccttag acagaatctt ataatctgaa
      961 cccacaacag cgtttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag
     1021 gtaatccccc tttcacactt tgtacatatc acagccaaag gacagaccag attgaagtgg
     1081 taaaaccgat cgtgcacact acgcagtctc acaactaaca agaggttatt atcctgatgg
     1141 atcaggttgt tgtttttcaa aagatgtttg agcaagtgag aaaagagcaa aacttctcct
     1201 ggttttattc agaattaaaa cattaccgta ttgcacatta catttattat ctggctacgg
     1261 ataacatcag aattattact cacgatgaca cggttttgtt attaagagga accaggaacc
     1321 tgttaaaagt tagtacgacc aagaaccctg ctaaaataaa agaggccgca ttgcttcata
     1381 tttgcggaaa atcaacattt cgggaatact gttcaacact ggcagacgcg ggcgttttcc
     1441 ggtgggttac tgatgttaat cataacaaac gcagttacta cgccattgat aatacgcttt
     1501 tatatattga agatgtagaa aataataaac cattaatcta gctaaagttg gatgcttaaa
     1561 aaatgcttca taattcagta aggcattagc ataatggaaa taaaagtg
//
LOCUS       DARNVM010000305         1607 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.307, whole genome shotgun sequence.
ACCESSION   DARNVM010000305 DARNVM010000000
VERSION     DARNVM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.307"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            324..689
                     /locus_tag="SI621_004727"
     CDS             324..689
                     /locus_tag="SI621_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4067333.1"
                     /translation="MIRIAFLITNKFLEIDSTTLAGYDFSGMNLHRATLNGYCLDGAK
                     FEKTHLRNVMIQHTSTRNAVFHNAALMNAILNNSDFTGSDCSNSRSIGENFKAIDNHG
                     KDIINGKGLSNVCKIHDNV"
     gene            661..1037
                     /locus_tag="SI621_004728"
                     /pseudo
     CDS             661..1037
                     /locus_tag="SI621_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1142..>1607
                     /locus_tag="SI621_004729"
     CDS             1142..>1607
                     /locus_tag="SI621_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEI4067334.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCKDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVI"
BASE COUNT          579 a          243 c          340 g          445 t
ORIGIN      
        1 ggtgaatgtc aaattctcat caggtataaa gaccgctgga ttgagaataa actcaaagaa
       61 aaaataattg aataaccatg tcagttatag acattttaga tggaacgaac cagagaaggt
      121 catgcctccg agctgggtta tgtggacacg gagctttagc gtagtgaact caacgaagca
      181 agccggaaac ctgccagccc ttatcagcac gtaagggaca acagcgtagt acgcagaata
      241 atcctaccca cgtagtatgg acacaggcct aagcgaggtt ctggttttca aatttttcag
      301 gagttagatg attggaggaa cagatgataa gaatcgcatt tttaataacc aataaatttc
      361 ttgagataga tagtaccact cttgctggat atgattttag cgggatgaat ctacatcgtg
      421 ccaccctcaa tggttattgt cttgatgggg cgaaatttga aaaaactcat ttaagaaatg
      481 taatgattca acatacatca actagaaatg ctgtctttca caatgcggct ctgatgaatg
      541 ctattctgaa taactcagat ttcactggaa gtgattgtag taattcaagg tcaataggtg
      601 aaaattttaa agcaatagat aatcatggga aagatataat aaatggaaaa ggattgtcta
      661 atgtttgcaa aatacatgac aacgtataag aaaatattat tcagaaccta tgtgattgta
      721 tctttgtttt tacatacaac acatgggcgc agtgtaataa tattattata atgcgcaagt
      781 atgaatgtga ggggaaatac accgttagaa atttggtaaa aaataaagct atagcattgg
      841 aattagctga gatgtatgtt aaaaatcgtt atgggcatga tgccgcagaa gaagaaaaac
      901 catacaaaat catggaatta ccaacaagtt ggattgttga aggcaccatt gaatcagacc
      961 aaattgctgg tagggttttt attatagaaa taggcaaaaa tgacgggaga attctaaatt
     1021 ttggccacgg aaaataagta tgtaaggata aacaaacatc gtgtgtgctg atggcagagt
     1081 aggtggagga ctccagacag tcaaacgata gaaaaagata gtctttatgg aggttcctgc
     1141 aatgtcaaat acataccaga aaagaaaggc aagtaaagag tatggtttat ataatcaatg
     1201 taagaaacta aatgatgatg aattatttcg cttacttgat gatcacaatt ccttgaaaag
     1261 gatttcatct gccagagtat tacagttaag aggtggtcaa gacgctgtta gattggcaat
     1321 tgagttctgc tctgataaaa attatatccg tagagatatc ggagcattta tactcgggca
     1381 aataaaaatt tgcaaaaaat gcaaagataa tgtgtttaat attttgaaca atatggcatt
     1441 gaatgataag agcgcttgcg ttcgagctac ggcaatcgag tcaacggctc agcgatgcaa
     1501 gaaaaaccca atttattcac ctaaaatagt agaacaatct caaattactg cttttgataa
     1561 atcgactaat gtcagacgtg ctacagcatt tgctatttct gttatca
//
LOCUS       DARNVM010000306         1427 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.308, whole genome shotgun sequence.
ACCESSION   DARNVM010000306 DARNVM010000000
VERSION     DARNVM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.308"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..446
                     /gene="rfaY"
                     /locus_tag="SI621_004730"
     CDS             <1..446
                     /gene="rfaY"
                     /locus_tag="SI621_004730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HEI4067335.1"
                     /translation="NLIVETDRVRSAGLTFPNDFYFLAERKFFNYASVFIMLIEYVEG
                     VELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTA
                     ERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
     gene            472..>1427
                     /locus_tag="SI621_004731"
     CDS             472..>1427
                     /locus_tag="SI621_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEI4067336.1"
                     /translation="MDLLAESITEVTVSGKITNTDRVLNIAYGIDRNFLFGAAVSMQS
                     VIMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNG"
BASE COUNT          437 a          251 c          301 g          438 t
ORIGIN      
        1 agaatttgat cgttgaaacc gatcgtgtca gaagtgcagg tcttactttt cctaatgatt
       61 tttattttct ggctgagcgt aaatttttca actatgccag tgtttttatc atgcttatcg
      121 agtatgtcga gggcgtagag ctcaatgata tgccgattat tccagaaaat gttaaagcag
      181 aaattaaggc ttcaatggaa aaattgcatg ccttaaatat gctatcgggc gacccacacc
      241 ggggtaactt tatcgttagt aaagatggtg tcaggataat tgacctatcc ggtaaaagct
      301 gtacggcaga gcgtaaagct cgtgatcgtc tggccatgga acgtcacctg ggtattgcca
      361 acgaaataaa agattatggc tactattcag taatctacag aaccaaacta cgcaaattta
      421 ttaaaaaatt aaaaggcaaa gcgtaaacca cacagtcaaa acggaaccaa catggattta
      481 ttagctgaga gtattactga agtcactgtc tctgggaaaa ttactaacac cgatcgtgtg
      541 ttaaatatcg cttacggtat tgaccgcaac tttttatttg gtgcggcagt atctatgcaa
      601 tcagttatta tgcataaccc ggaccttgcg gttaagtttc atctctttac cgactacatt
      661 gatgaagatt atctacaacg tgttaacgct tttaccagca aaaatgctaa cgttgaagta
      721 agaatttata aagtctccag tgcctttatt gatatcttcc ccagcctgaa acagtggtct
      781 tatgcaacat tcttccgttt agttgcgttc cagtatctga gtgaagttat tgaaaatctg
      841 ttatatatcg atgctgatgt catctgtaaa ggctcattag ctggattgct tgatattaat
      901 tttgatggcg ataagttcgc agctgttatt aaagatgtgc cttttatgca ggaaaaacca
      961 gcgaagcgtc tggctataga gggacttcca ggtaattatt tcaacgccgg tgtagtatat
     1021 ctgcagcttg aagcatgggc gaaaaatgat tttatgaata aagccattgc tatgctggca
     1081 agtgacccgc agcatacgaa gtataaatgc cttgatcagg atattttaaa tattctgttc
     1141 tttggtcatt gtatttttat tagcggcgat tatgattgct tttatggcat tgactatgag
     1201 ttaaaaaata aaagcgatga agattataaa aagaccatta ccgatgatac taagctgatt
     1261 cattatgttg gcgtaacgaa gccctggaac gactggacga attatccctg ccagaagtat
     1321 tttaatgagg cttatcaggc ttcttgctgg aatgatgtgg cgtttattcc agccacgaat
     1381 gaaaagcagt atcaagtgaa atccagacat ttgaaaagaa atggtaa
//
LOCUS       DARNVM010000307         1425 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.309, whole genome shotgun sequence.
ACCESSION   DARNVM010000307 DARNVM010000000
VERSION     DARNVM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.309"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..226
                     /locus_tag="SI621_004732"
     CDS             <1..226
                     /locus_tag="SI621_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEI4067337.1"
                     /translation="EVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGD
                     KTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            254..723
                     /locus_tag="SI621_004733"
                     /pseudo
     CDS             254..723
                     /locus_tag="SI621_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          422 a          276 c          350 g          377 t
ORIGIN      
        1 ggaagtccgt attgaagcaa taatcggact ttcctacaga aaagataaaa gggttttatc
       61 tgttttatgc gatgagttaa aaaaaaatac tgtttatgat gatatcattg aagctgcggg
      121 tgaattaggt gataaaacgc tacttcctgt tttagatact atgttgtaca agtttgatga
      181 caatgaaatt ataacttccg ctattgataa gctgaagcgt tcatagcgtg agttgcctat
      241 gcacagtggg ggattcccgc cggcacggtg cagatgctcg accggctgga aagtgaaatc
      301 ctggctgacc gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgacggtg
      361 aagcagatgc aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac
      421 cactgcgacc atcgcggcct gccgctggtg cttatcagca cggaaggggc aacagaatgg
      481 tgcgcagaat acgatgaatg gggcaacctg ctgaatgaag agaacccgca acagctgcag
      541 caactcatcc gcctgccggg tcagcagtat gatgaggagt ccggcctgta ttacaaccgc
      601 caccgctatt atgacccgct gcaggggagg tatatcactc aggattcgat tgggctgaag
      661 gggggatgga atttttatga gtatccgttg aatccggttc agtatataga cccttttgga
      721 ttagctgata tgaatttcta tgatgagggg atacgcagaa atgtatcact gcatgctgca
      781 acagataaaa taaatattcc tggcgttttt actattggag gacatggata tcctggtttt
      841 attacagatg aaaatggaaa ctatgaaact gctgagggat tagccaagag aatcagagct
      901 aatccacgct ataaaaaggg aatgtctgtt tgattattct catgtggtac agccagagag
      961 ggagataaat cttttgccag caagttagcc agagcacttc atgcaaatgt cactggagct
     1021 gatacacaat ggcaagtatg gatggcgtgg gataataata ctcctctttt taatacgatc
     1081 caaacaggac ttacggataa tatctcggca attgacacaa gtaataatgt cataactggt
     1141 aataatatgg gccgctggat aacgtttgga ccttcaggtc aacccatttc ttccactcct
     1201 ggcaaagtta tgagaccaag tgatataaaa taacaaaggt ggcaacatga agtgcttttt
     1261 atttattctg attgcaaatg ttttcttttt ctcttgttgt tcatatggag gtgaaatccc
     1321 cccccctatt aagctccata acattgaaat aagctcaaca tctgaatcgt ttaatttaag
     1381 tgaaaatcaa agatatgaac ttgatattaa atctgagggc gggga
//
LOCUS       DARNVM010000308         1422 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.310, whole genome shotgun sequence.
ACCESSION   DARNVM010000308 DARNVM010000000
VERSION     DARNVM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.310"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..581)
                     /locus_tag="SI621_004734"
     CDS             complement(<1..581)
                     /locus_tag="SI621_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEI4067338.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHTSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFA"
     gene            complement(1285..>1422)
                     /locus_tag="SI621_004735"
     CDS             complement(1285..>1422)
                     /locus_tag="SI621_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEI4067339.1"
                     /translation="YNLNKAIYWMEFAVNNGNIDAKSKLQDLKKLKLKRMDRRKNKEN
                     P"
BASE COUNT          418 a          302 c          245 g          457 t
ORIGIN      
        1 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc
       61 atcgcatcag ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata
      121 gctgtaattt cattggattt ctcatccgtc ctgatctgtc cgatgaccag actgtgcatt
      181 gttgagaacg cactaatgac atgaatcgct cccttgcggc gactcttgtc ataagaatgc
      241 cggagtgttt ttccatcaat tgcgatgacg tctttatcat ctgaagtatg gcaatctcgc
      301 atccagttaa taaagcactc gtgaaatttt gcaggactga tacaggatac aactctggcg
      361 atggtatcgt gaacaggaat accattttca aaatcaccgt attgttttaa aaaatcgaga
      421 tgtgtttccc caaaatcctc tatatcttcc caaccttctg cacccgaaat aacggcacaa
      481 atagtcaaca gtagaatatc cgataattta tgttccactt tccaggcttg tctgtaatcg
      541 gggataatag aaatatgctc catcaatttt ttaagttcca ttttgttctc cttaattatg
      601 taaggagtat ttgatcatgt ataagcaata aaaaacagct ttaggtaatg aggaatatct
      661 caattttcaa acataaaatg cgaattattt agtacaaaaa agcagcgtta cgtgatcttt
      721 ccctggcatg tcattagatg aaccagttat acaaccctcg gaacagaatc gaggtcctaa
      781 agacggacca aaagcgttta aacaactatg tattgaaaat ccattacgat tataagttcc
      841 tgtattaact gacggtacta atcgtatgcc gtcaggtttt ctattgcttg ctcccgggtc
      901 attaacattc caactccata ggccttgagg tattggccct ctatttataa atgtgtacaa
      961 cggggattat ttttacattg catattatta ccagaagcga ctggaatact aagcacatca
     1021 taaaaattat catcacctgg tatatctgaa acacacgaaa gcatacctaa agttatgctg
     1081 tataaacagg tggctaatcc caaggggatc gattccagaa atcgggttca acggatactg
     1141 ataaaaattc cacctacctt ccagcccaat cggagcctga gtgttatacc gcccctgctg
     1201 cggttaatgt taccgataat agttatagta cagccctgat tcatcatcat actactgctg
     1261 cggcagtctg atgaattatt gcttttacgg attctcctta ttcttccttc tatccattcg
     1321 ctttagcttt agctttttga gatcttgaag tttgctcttt gcatcaatat taccgttatt
     1381 tacagcgaat tccatccaat aaatagcttt attcaaatta ta
//
LOCUS       DARNVM010000309         1403 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.311, whole genome shotgun sequence.
ACCESSION   DARNVM010000309 DARNVM010000000
VERSION     DARNVM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.311"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            207..1151
                     /locus_tag="SI621_004736"
     CDS             207..1151
                     /locus_tag="SI621_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067340.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSVP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
BASE COUNT          430 a          294 c          253 g          426 t
ORIGIN      
        1 accatatgcc cgttatgtta ttgtttcatt agtaataatc acttaaatgt atcgatgaaa
       61 caggtatgac ttagcgggag tgattgtttc tgagtgaaca gaggccactt tcgcctgata
      121 aatgaaaaat gagcgatttt tgcaagttag tttttctata gccgagtacg ctcagcgcct
      181 cgcttgatta caatgaggtt gctgctatga gtcctgataa tatcggttcg gctgtcaatc
      241 gcttctcagg ttggccatct aatgattctc taaaaacaga taaagaatca atgacatcac
      301 ccgtgactgt gcaggtaata aagatgattg ccgccatcct tgacccacat cggatagtcg
      361 ttggaatcga tacactttat acaacgcagc gcgaaacatt tacctcagag agtcaacagc
      421 tcattgaatc agcattgcaa acacttcatg tgatgaattt aaataacctg gaaacaaatc
      481 tcgatgagga aggtcactat ctggcttttg gatataacgt tgacactcct gacagaataa
      541 ccgtttctgc ttataatgaa aaaaagcctg gctgctatgg ccaatgtctt ttaatacagg
      601 atgacgaaac agacttattt aacctgataa aagttaaaat ttttgaagcc aggcataatt
      661 atgtcgctca attgatgagc gttccggaat ttatgttcaa atttagttat agcgctcaac
      721 aaaatagatt agaacctcat cttctggttc ccacttattt ccctctaaaa acaaacgaac
      781 cggttacgca agaggatgta ctgttacttt atcgtttctt taaaatggat ggtgatttta
      841 atatattaac atcagatgaa tacatgagta tcatgacacc tctaatgaag tgtgagcgta
      901 gcatccacga ccacaacagg tatgtgacgg gcaaagatac ccttcttctg gactatcctc
      961 cttctggtaa ccagatatat ttccatattt ttcctgacga atcagcaaca ctagtccttt
     1021 atttttcaaa caatgacatt gaatgtttcg tctttgaaag agatattccg agccagtatc
     1081 gctttttcaa aatgtttacc aatctggcac tggttattga tcagttaacc gatgccaatc
     1141 aaatacttta atttttatat attccctgtg gcaaatattg ccaaacaatg cccggggatt
     1201 aatttttatt actcacggaa agatttaaat aatgatccca tctcaaatct cgttcaacac
     1261 ccttcctgtc aatgcaacat acgcttcaga gacaacagtt gacacacaaa aattctctga
     1321 catcctctac tcagcggggt gttccctaaa ggatctggct tatctgagca gatgtctggc
     1381 atcaatacat ccgactatcg cca
//
LOCUS       DARNVM010000310         1401 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.312, whole genome shotgun sequence.
ACCESSION   DARNVM010000310 DARNVM010000000
VERSION     DARNVM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.312"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            27..1396
                     /locus_tag="SI621_004737"
     CDS             join(27..507,507..1396)
                     /locus_tag="SI621_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HEI4067341.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFVVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
BASE COUNT          427 a          285 c          333 g          356 t
ORIGIN      
        1 gacgatgaaa tggaatagcc cctaatatgt caaagccaaa ataccctttt gaaaagcgcc
       61 ttgaagtcgt gaatcactac ttcacaactg atgatggtta caggatcatc tcggcacgtt
      121 ttggtgtccc ccgaacccag gtcaggacat gggttgccct ctatgaaaaa catggagaaa
      181 aaggtttaat tcccaaacct aaaggcgtta gtgctgatcc agagttgcgt attaaggtcg
      241 tgaaagctgt gatcgagcag cacatgtccc ttaatcaggc tgctgctcac tttatgcttg
      301 ctggtagtgg ttctgtagcc aggtggctga aggtctatga agagcgcgga gaagctggtt
      361 tacgcgcgct caagattggc accaaaagaa acattgcaat atcagttgat ccagaaaaag
      421 cggcatcagc attggagctg tcaaaagacc gacgcattga ggatcttgaa aggcaagttc
      481 gatttcttga aacgcggctt atgtatctaa aaaagctgaa agccttagct catcccacga
      541 aaaagtaaaa gtactcaacg agctaaggca gttttatcct cttgatgagc ttctcagggc
      601 tgcggagata ccgcgcagta cgttttatta ccatctaaag gctctcagca agcctgacaa
      661 gtatgcggac gttaaaaagc gtattggtga gatttatcac gagaatagag gccgatacgg
      721 ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa cagattaacc ataaagctgt
      781 tcagcgcctg atgggaaccc tctcacttaa agcagcgatt aaggtcaagc gataccgctc
      841 ttacagagga gaggtagggc aaaccgcccc taatgttctc caaagagatt tcaaggctac
      901 gcggccaaac gagaagtggg ttaccgatgt tactgaattt gtagtcaatg ggcgcaagct
      961 gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca gcctttcgga
     1021 aagaccagtg atgaacatgg ttgagaatat gctcgatcag gcattcaaaa agcttaatcc
     1081 tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga gaagatatca
     1141 aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca attgtctgga
     1201 taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt atcttgatga
     1261 gttcagtaat ataagcgaac tgaaggatgc tgttacggaa tatattgaat actacaacag
     1321 cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga atcagaccta
     1381 tatgcctcgt gtttaactgt c
//
LOCUS       DARNVM010000311         1400 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.313, whole genome shotgun sequence.
ACCESSION   DARNVM010000311 DARNVM010000000
VERSION     DARNVM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.313"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            3..1034
                     /locus_tag="SI621_004738"
     CDS             3..1034
                     /locus_tag="SI621_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEI4067342.1"
                     /translation="MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTI
                     TPEQEKQYREKLTDALNAGYAVLNSGGSSLDAVQRAINVMEDSPLFNAGKGAVFTHDG
                     KNELDAAIMDGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGL
                     VMVEPAYFKTDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDK
                     DGNLAAGTSTGGMTNKRYGRVGDSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNI
                     AAQVKYKNTPLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSFNTEGMYRGTIGNDG
                     KAIVSIYEQ"
BASE COUNT          471 a          263 c          285 g          381 t
ORIGIN      
        1 ttatgaataa aataaaaggt ataacagtaa tggcagcctt cgcattgtgt ataggatatg
       61 ctcacgctga atctcaaacg ccaatacgcc ttgtaattca tggtggagct ggcaccataa
      121 caaaagatac tattacacct gaacaagaaa aacaatacag agaaaagtta acagatgcac
      181 taaatgctgg gtatgctgtg cttaatagtg gaggaagtag ccttgatgct gttcagcgtg
      241 caattaatgt tatggaggac tctcccctct tcaatgctgg aaaaggcgct gtctttactc
      301 atgatggcaa aaacgaactt gatgctgcaa ttatggatgg aaaaaccaaa atggccggag
      361 ccgttgcagg agtgacgacc ataaaaaacc ctataaacgc tgcttatgca gttatgacta
      421 aaagccctca tgtattaatg atctctaatg gtgcggaact atttgcaaaa gagcaaggct
      481 tagttatggt tgagccagca tatttcaaaa ctgatttccg ttggcaacag ctacagaacg
      541 ctttaaaaga cgaaaaaata acccttgatc ataatggtaa atcagcatct cttttactcc
      601 ccccaaagaa ctacgactat aaatatggaa ctgttggagc agtcgcgttg gacaaagatg
      661 gtaatctggc agctggaaca tcaacaggag gtatgacaaa taaacgttac ggacgcgtag
      721 gtgactcgcc aataattgga gcaggaacct atgccgataa taataccgtt gctgtttctg
      781 ctacaggtac tggagaaatg tttattagaa cttcaactgc atacaatatt gctgctcagg
      841 tgaaatataa aaatacacca ttgaaagaag cagctcaaaa tgcgcttgat gaagttaaaa
      901 acattaatgg tagtggtggt gtcattgttc tagataagaa tggtaattat actatgagtt
      961 tcaatacaga agggatgtat cgtggaacta tcggcaacga tggtaaagct atcgtatcaa
     1021 tttatgaaca atagataaaa tattttatac atcgtcttgc ccttaaggcc cattttaaaa
     1081 gcgggccttt atttaataaa tattcttaac gcctccttat ttcacagcta aaagaagagt
     1141 aattcttaga ctaattaacc gtctctctac cgagacgctt acccatatcc agcccgtacc
     1201 ctgcagaaat accgcgatgt atagcgtttc tgtgtcatgg tgaaaagtac aggaggaaat
     1261 tataaattct gggaataccc ctggttgtaa cattttgtaa tacattaagt gatatcatta
     1321 tttatgatgt aaaaaggggc ctgcaatagc ccctgaaaaa tcagaaaacc ttcattttat
     1381 acctgcagcg ctcattgact
//
LOCUS       DARNVM010000312         1396 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.314, whole genome shotgun sequence.
ACCESSION   DARNVM010000312 DARNVM010000000
VERSION     DARNVM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.314"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..652
                     /locus_tag="SI621_004739"
     CDS             <1..652
                     /locus_tag="SI621_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEI4067343.1"
                     /translation="KRKELFESIKDYLLDEANRKADGRIETETELATRFNTSRYKVRQ
                     ALSTLVQMGMLERAPRRGSLLKNVGQSSINDQLLMQFDLSGFDISEFTEARILVECAI
                     IPLATRRIFPAMLSKLENELRLIEENADNPQIADQHDRDFHLLLLQASGNRVLQVFSD
                     VLIAYFEKTRESLPDNDCQYFLDTASKQREILSYIKKGDAQMASELMRVHLLEKKI"
BASE COUNT          444 a          247 c          297 g          408 t
ORIGIN      
        1 taaaagaaaa gaactatttg aatctattaa agattatttg cttgatgaag ctaatcgtaa
       61 agccgatggc cgtatagaaa cagaaacgga acttgcaact cgttttaaca cctcccgata
      121 taaagtccgt caggcactta gtactctcgt ccaaatggga atgcttgaac gagcccctcg
      181 cagaggaagt ctgttaaaaa acgttggcca gagtagtatt aacgaccaat tgttgatgca
      241 gtttgatcta tctgggtttg atatctcgga atttacagag gccagaattt tagttgagtg
      301 cgcgatcatt ccactggcaa cacgccgaat tttcccagcg atgctctcca aacttgaaaa
      361 tgaattgaga ttgattgagg aaaatgcaga caatccacaa atagcagatc aacatgatcg
      421 ggattttcac ctgctcttgc ttcaggccag tggtaatcgt gttctgcagg ttttctctga
      481 tgtgttaatc gcttattttg agaaaacaag agagtcgtta ccggataatg attgtcaata
      541 cttccttgat actgcttcaa aacagagaga gatattgtcg tatatcaaaa aaggcgatgc
      601 ccaaatggct tcagagttaa tgcgagttca tttactggaa aagaaaatat agcattaaat
      661 ctttcaaaaa ctaaaactca accggaaaac ttatctggtg tgagtgctat gtgatatcac
      721 tgtcaagtta ttagctgtgt ttatcctaat aaggtatctt tgatatataa gcgagcttta
      781 ttggtaaaga gagcgctatc atagcagact atgttaagtt agagcaggga tgcgtaagtc
      841 tgagtgccac gtcttctgta cgtatcgacg ccttatacga tctggaaaat attatcaggc
      901 ataaacataa aggagaactt gcctgagtag ttgccttttg agaaatttat cttcactgga
      961 tgggagttac ctgccaacat gtgttcccgt ccaaggacag aaccaacagt tgggtgttga
     1021 ttacctggtg acgcggaacc aacagaagtt gtaattatgc aggtctgagg agatcagtat
     1081 cagagatatc cgaatgaact gtgataatgg tttgacaaaa acggataaca atacaacaga
     1141 acacgttctt agtaatctca gccatggaaa taaaaattag atgtatctcc aatcagtgag
     1201 ttgcgtggaa attacaaggt tctgctgtac gatctgagtg ggttaacttg atattttgta
     1261 ctcgtcacag aaaatataca gagatataca ctctggtgta atcactgttg taggttgatc
     1321 aggtgtcact ctttttacta taatgtttag tataagcaat atttcatttt caaaacgtac
     1381 ttaaaagcac aattgc
//
LOCUS       DARNVM010000313         1379 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.315, whole genome shotgun sequence.
ACCESSION   DARNVM010000313 DARNVM010000000
VERSION     DARNVM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.315"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            398..1258
                     /locus_tag="SI621_004740"
     CDS             398..1258
                     /locus_tag="SI621_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HEI4067344.1"
                     /translation="MASTKGTLNHAIASLDSSAPEVRSSATSSTLTASSSNEFKSDKY
                     ICSDIADLMSVLGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFD
                     VNAFQKALMHFYYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLP
                     VIPPDPKHRTPSPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISK
                     EFTGYEISTAEYQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITAL
                     FDSAKDYLRF"
BASE COUNT          460 a          293 c          269 g          357 t
ORIGIN      
        1 gcctcatcaa ctggaaaaca gcaaaagcga gggtaatttg tataacagcg ccagtatgga
       61 taataatatt ggcgctatgc tcaagaaaga tgttgaaaaa ctatcaactt tagtcagtga
      121 acgtagcccg cgaaatgaag actggaaatg ccagcatgac atacaactca acttcgttaa
      181 aaacatgctc agaaatttcc caactgagaa cctgtcagag aagggatctg cgctcttaca
      241 cgaaagttat tcgctgataa aaaacgttct gcagcttaca gaaaatgtta atactattga
      301 cggaaaatta aaggatcccg ctaaaatatt gaccttgcat aactctgtga gaaaaaccct
      361 ggctaacgtt gctgacaaca caaccgggtc aagtgcaatg gcatcgacca aaggtacctt
      421 aaatcacgct atcgcttctt tagatagttc tgcacctgaa gtgagaagtt cagcaaccag
      481 cagtacttta acggccagta gttcaaacga atttaaaagt gataaatata tatgcagtga
      541 tattgctgat ttgatgagtg tgctcggtag cgactacctc gaaatttatg cgaatagcgt
      601 tgagataatg agcgcatact ggcaggactt cagtgaacat atccaaagca atatgggaaa
      661 atggactcac tctaataaaa aaggcgatgc gatagttttt gacgtcaatg cattccaaaa
      721 agccttgatg catttttatt atatcgataa atatcctaat ggtgatttcc actatcatta
      781 caatccagac tatgttttat accctccggc acctgcggac aaaattggcg ttccactgga
      841 agaagcagaa aaatggtgcg ccgctttagg acttccggta attccacccg atcccaaaca
      901 ccgcacacct tctccgattg tcgaagttga accgcaaggt agcggtctgt atgttattat
      961 tcccaaccct cagattatag acagtatgag tcagtcttca gactcaatgg tgcataggga
     1021 cgataaaggg aaggaaaaaa atatatctaa agaattcacc ggctacgaaa taagcacggc
     1081 tgaataccag gcatggctgg cgggttataa cagccaggca gaaaacatga aaaccgacgt
     1141 gcaggttatc acgactaaat acagtacagc caactccacc tacgatacaa ttatcaaatt
     1201 attatcttca accattactg cgttgtttga ttcagcaaaa gattatttac gtttctgatt
     1261 aagtaacatc cacaattatc gcccactatc tggtcccgaa aaatgggacc agcgtttcat
     1321 attattaagc atcagctttc attcatttct tcatcacaat cttacaaaat gagcgtttt
//
LOCUS       DARNVM010000314         1358 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.316, whole genome shotgun sequence.
ACCESSION   DARNVM010000314 DARNVM010000000
VERSION     DARNVM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.316"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..334)
                     /locus_tag="SI621_004741"
     CDS             complement(<1..334)
                     /locus_tag="SI621_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067345.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLM"
     gene            complement(566..715)
                     /locus_tag="SI621_004742"
     CDS             complement(566..715)
                     /locus_tag="SI621_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HEI4067346.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
BASE COUNT          421 a          305 c          278 g          354 t
ORIGIN      
        1 ccatcaatct ggataccaga tacgaactcc tgtggctctg accgttcaga tagcccacaa
       61 aatgaatgtg aagcccttta cgttccgtat attccattac ccaggcatac ccgaccagtt
      121 tctctcccac ttcgttaatc agtcgcagta tctccgcgca catggcgtac ctgcaggcac
      181 atctgtagct gagggtgttt tttcggtaag caaaatccat tctgaacggt aacagtttcg
      241 aatatacccc gaataatgaa tccagatgct ggttaaagtg tgtcaggagc tggggatcca
      301 tttcgtaacc gggattggtg ttgtaagtct tcatgtttct tcctcgtgtt ctgtaacaca
      361 ggaagaagcg ctggcgtaat ttttgaagaa aacgttatca cacgaagacg tgcaggttca
      421 caactccccg ggcgccacag agaaagtggg accacacccg ggtatcagaa ccatacagag
      481 aacggcatac gtgacagtca atcacagcgc agataatatc tgaagtgatg tcattgtgac
      541 gactgtactt catcagaaac atttatcagg taacatattt ccagactgaa gcgggaacac
      601 gatcgtaaag tttgttcatg gtcagttctg ccagccggtg atcgaccgca gagttgaagg
      661 cttccatctc ctctggagta agcttataac gcgcgtggga aatcactttt tccagtgaac
      721 aacagtggaa ctactctggt ttaaaattcc attaccctct cgttatcttg tactgtgatt
      781 aacgtaatat cgtcataatg gcctgacgtt atcccgaaag cctttccact tcccagtttc
      841 tccggtaaaa gatgttttta atactgaagg cataacgcca taaccagata ttacaattaa
      901 cgtagctttc cgcaacgtac tacatgagta acagaggtac agggttaaac aacactccgg
      961 aagacttttt aatgacatga ggcatactct tttacgtatt actaccaaaa ctcatcaggt
     1021 gagctgcagg aacagatcat taacagtaat tccttaatca ccgttatcca cagtatcaac
     1081 actggcgaag tcatactctt agctataaag gtgatgacgg gttcagaata gttattacac
     1141 catacaacaa aatacagaaa acaacaggaa aaataacaga aaaaaatagc agacattact
     1201 taacaaagca cgttacagtc agtgtgtgaa cattacagat ttatattcag gtgttgcggg
     1261 actacattac tgaagagaga gttaaggaca gagttacaag tactactaca gagaatatca
     1321 ctgcacagat acttctatga gacagacttc tacaggac
//
LOCUS       DARNVM010000315         1346 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.317, whole genome shotgun sequence.
ACCESSION   DARNVM010000315 DARNVM010000000
VERSION     DARNVM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.317"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..845
                     /locus_tag="SI621_004743"
     CDS             <1..845
                     /locus_tag="SI621_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEI4067347.1"
                     /translation="EANGSRGNKTRLAYAGLKISEFGSLDYGRNYGVGYDIGSWTDML
                     PEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQGKNERTDNGHLYGA
                     DYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAELWATGI
                     KYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAYLKSKGR
                     DLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDNIVATGI
                     TYQF"
     gene            1009..>1346
                     /locus_tag="SI621_004744"
     CDS             1009..>1346
                     /locus_tag="SI621_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEI4067348.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLAQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLEKNCLTCLDFT
                     DNASLVNINL"
BASE COUNT          390 a          295 c          278 g          383 t
ORIGIN      
        1 atgaagccaa cggctcgcgc ggtaacaaga cccgtctggc ctatgcaggt ttaaaaatta
       61 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctatgac attggttcat
      121 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
      181 cataccgtac taccggtgtg gcaacctatc gcaactacga tttcttcggc ttaattgaag
      241 gtctgaactt tgccgcgcaa tatcaaggca aaaatgaacg cactgacaat ggccatcttt
      301 atggtgctga ctacacgcgt gccaatggtg acggtttcgg tatctcctca acttatgttt
      361 atgatggctt tggtatcggt gcggtgtata ccaaatccga tcggacaaat gcgcaggaaa
      421 gagccgctgc taatcctctc aatgcctccg gtaagaatgc agaactgtgg gctacaggta
      481 taaaatatga tgccaacaac atctactttg cagctaatta cgctgaaaca ttaaacatga
      541 ccacctatgg cgatggttat atctctaaca aagcacaaag ttttgaagta gtggcacaat
      601 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
      661 tgggccgcta cggcgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
      721 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
      781 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc aacgggcatt acctatcagt
      841 tctaacatcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
      901 atgactcctt aagtaaaaat gagcaatttt tgatatttct ggtcattcac ctcaattaat
      961 attattccat accctccggg gacatcttac tataatattt attgatgagt gattatgacc
     1021 aatattaata cagcttgtgt aaaaaataat gccagttatc aattaaataa cgcattacct
     1081 aacaaagaaa ccatctccag caatttttgt gaacgactgg cacaatgggg taataagtcg
     1141 cttaataatg gtgaagaaag agctattgcc gtagagcgaa ttaaagaagc ttacaattcg
     1201 aacatggcat ctcttgattt gtcatatctt gatttgagtg agttaccgcc tatcccctcg
     1261 acagttaaca cgttaaatct ggaaaaaaat tgtctcactt gtcttgactt tactgacaat
     1321 gccagcctcg tcaatatcaa cctcag
//
LOCUS       DARNVM010000316         1338 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.318, whole genome shotgun sequence.
ACCESSION   DARNVM010000316 DARNVM010000000
VERSION     DARNVM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.318"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     repeat_region   72..1320
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=133..161
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
BASE COUNT          268 a          390 c          372 g          308 t
ORIGIN      
        1 tacgtggata tgttgcttaa tacgagtact gctaatataa aaacttgaga aagagataac
       61 gggttatatg gtggtttatt cccgctggtg cggggaactc atcagtgata tccaacattg
      121 cggagctaaa cgcggtttat ccccgctggc gcggggaact cacgaacgcg gcacaccagg
      181 gcgtctcatc gtccggttta tccccgctgg cgcggggaac tcggcacatg cctgatcata
      241 tgtgtagcaa cggtcggttt atccccgctg gcgcggggaa ctcacgatgt atgaccggaa
      301 aacctcagac tggcgcggtt tatccccgct ggcgcgggga actcccattc tggaatttgt
      361 aatctggact cccatgcggt ttatccccgc tggcgcgggg aactcatcca gcgcattcct
      421 gtgtgccgta tctaaatcgg tttatccccg ctggcgcggg gaactcagca ccatttaccc
      481 accattgctc aacggtaccg gtttatcccc gctggcgcgg ggaactttgc cactggtttc
      541 atgcaggccg cgcaaaaatc ggtttatccc cgctggcgcg gggaactcca attcatatac
      601 tgataaatca tcaaaacaaa cggtttatcc ccgctggcgc gggaaactcc ccctgcgctt
      661 gatgaacgac aacaagaatg gcggtttatc cccgctggcg cggggaactc gcagtaccat
      721 tacatcaaag agtgcagaat cccggtttat ccccgctggc gcggggaact cggtgctgtc
      781 tttaccgtac attgcgcgaa tctcggttta tccccgctgg cgcggggaac tcatatcctt
      841 attggaatac atgagctgcg cccacggttt atccccgctg gcgcggggaa ctccgcgaca
      901 cgctggcaga gcagctatcc caccacggtt tatccccgct ggcgcgggga actctttttc
      961 cagctccagc attgctgttg tattgacggt ttatccccgc tggcgcgggg aactcttaat
     1021 caggtcgtcg tactcagcgc cgctggccgg tttatccccg ctggcgcggg gaactcagca
     1081 cggcaggcca tatgaaatac ctgttgctcg gtttatcccc gctggcgcgg ggaactcgca
     1141 cccatcaagg aataacgcgt ttattttccc ggtttatccc cgctggcgcg gggaactcta
     1201 atatccctgg cgataatcaa ccggcttact cggtttatcc ccgctggcgc ggggaactcg
     1261 agagcgaggg gagattccgg cagggcgtgc acggtttatc cccgctggcg cggggaactc
     1321 tcaataatct gattaccg
//
LOCUS       DARNVM010000317         1317 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.319, whole genome shotgun sequence.
ACCESSION   DARNVM010000317 DARNVM010000000
VERSION     DARNVM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.319"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(19..234)
                     /gene="essD"
                     /locus_tag="SI621_004745"
     CDS             complement(19..234)
                     /gene="essD"
                     /locus_tag="SI621_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HEI4067349.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(1013..1087)
                     /locus_tag="SI621_004746"
     tRNA            complement(1013..1087)
                     /locus_tag="SI621_004746"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1052..1054),aa:Gly,seq:tcc)
     gene            complement(1091..1165)
                     /locus_tag="SI621_004747"
     tRNA            complement(1091..1165)
                     /locus_tag="SI621_004747"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1130..1132),aa:Thr,seq:tgt)
BASE COUNT          383 a          325 c          266 g          343 t
ORIGIN      
        1 tagttttgag tcattgaatt actctccccg tgcagccttg cgcttatctt ctttaatctt
       61 gaaataaagg tttgtcaggt acgtcagcag gccaaatacc agactaccca gcacaccgat
      121 tgcagcccac tgtgacggag ttactctatc gagcaactgt aaaaaccagt agccagcact
      181 gcctgcggag gtgccatagg cgacacccgt tgttaactta tccatggatt tcataactcc
      241 cacctcgcag atgcgggcgc tgtgtaacgg aaacaaaaaa tggccaccag cggcccgtaa
      301 aaaacacccc gtcaaaagca ccggcatccg cagatgccct ttgcgtggcg ttatttgatg
      361 cgcgccagat gtggcgcaaa gaaatgaaat aagacttatc gaaaattaag gttaatttga
      421 tgatttaaac cacttctgaa gcttagtagt atggacatgt ccccagaagg gggccaatac
      481 ttattattct tcatggactt tgtcccgcgg tcttaatccg acgaccgcgc tacttttcac
      541 cctctcgcaa attgctatct aaaggacgtt gtcccacgag gattcctgga tgctcgtgtc
      601 tttttttgtc tgatgcaggt ataaaaaaaa ccgccagata tggcggttgg tcaatgtata
      661 agataaatca ttttaattgt aataaaaatc gaggtgtcgg gtgcctcccg aaatatctgt
      721 ccctacaaca aatattgtga ttccccgcta aaccactata taaaccaccc tcgcactgag
      781 gaacacctct gtggtgcttt tacaacacca gaatgatgca tcaccgaccc tgccaggaaa
      841 tacaaaatct ccaccgataa tgcaccattc tgctgtcgta aaaaaatcag cactgaggct
      901 acacctggcc tcaaattata gccagagaac agaatgcttt tccaaaacaa cctgctccca
      961 cgtaataaaa aatacgccag tgccgcaata caataaggct tgtttcaaat gctggagcgg
     1021 gtagcgggaa tcgaacccgc atcatcagct tggaaggctg aggtaatagc cattatacga
     1081 tacccgcata tggtgccgac taccggaatc aaactggtac ctactgatta caagtcagtt
     1141 gctctacctg ctgagctaag tcggcattgg ttcttcaggg gagcgatatc accgatcaaa
     1201 gaagagttcc ccctcagaac cgttttcgat aatacgattt aatattccaa tcgcaacaac
     1261 actttgcgtc aaattatgta aatttattta tgtattttta ttttatgtga acatttc
//
LOCUS       DARNVM010000318         1285 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.320, whole genome shotgun sequence.
ACCESSION   DARNVM010000318 DARNVM010000000
VERSION     DARNVM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.320"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..439
                     /locus_tag="SI621_004748"
     CDS             <1..439
                     /locus_tag="SI621_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067350.1"
                     /translation="AKRNVGNGENQIPDMSFWTVTGGNGNFVIRQPDGLITQMVTVSI
                     SGPVAMNGMTDNAYAITGSNKSYIATATLPFVFPNKVLGVIPLVSTTAYGGVSSNITG
                     SYATAVCSFAAVRGNNTIVFKVDKPLNAAFPSDTSVSALIIGR"
     gene            442..885
                     /locus_tag="SI621_004749"
     CDS             442..885
                     /locus_tag="SI621_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEI4067351.1"
                     /translation="MNSVFFSPGSKSFYLQELFPEYEDAGTLPDDVIEITRETYEQFL
                     GLHPEGKEIGADSSGRPIWINSPPPSKEDEVLMAEMKKISLVSEANTYINTHQWPGKA
                     AIGRLKGNELEQYNLWLDYLDALELVDPSSAPDIEWPTPPAVQAR"
     gene            complement(857..>1285)
                     /locus_tag="SI621_004750"
     CDS             complement(857..>1285)
                     /locus_tag="SI621_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEI4067352.1"
                     /translation="GYAICRSVDLNSWEYMPDHRGEIIYSTETGEAKEITAPGDYPEN
                     TTTIVPLTPYDKWDGEKWVTDSEAQHGAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTQLNAVLDYIDAVTATDTSTAPNVIWPELPEA"
BASE COUNT          327 a          287 c          306 g          365 t
ORIGIN      
        1 ggcaaaacgg aatgtgggca acggggaaaa ccaaatccct gatatgtctt tctggacggt
       61 tactggtggc aatggaaatt ttgtgattcg tcaacctgac gggctaatca ctcagatggt
      121 tactgtaagt ataagcggcc cagtggcgat gaatggaatg actgataatg cttatgccat
      181 tacaggttct aataagtctt atattgccac agccacattg ccctttgtat ttcctaataa
      241 ggtactgggc gttatccctc tggtatcaac aacagcctat ggtggtgtat ccagtaatat
      301 tacaggttca tacgcgacgg cggtttgttc ttttgccgct gtccggggga ataatacgat
      361 tgtattcaaa gtcgacaaac cactgaatgc agcctttcct tcagatacca gcgtctcagc
      421 gttaatcatt ggacggtaat aatgaactca gtattctttt cacccggaag taaaagtttt
      481 tatctacaag aattgtttcc agaatatgag gatgcgggaa cgcttccaga tgatgttatt
      541 gaaattaccc gagaaacata tgagcaattt cttgggctgc atccagaagg gaaagaaatt
      601 ggcgctgaca gttcaggacg gccaatatgg attaattccc caccgccttc aaaagaggat
      661 gaggtgttga tggctgaaat gaaaaaaata tctttggttt cagaagccaa tacctacatc
      721 aatacccatc agtggcctgg caaagctgct attggtcgtc tgaaaggtaa cgagctggag
      781 caatataatt tatggctgga ttatctggac gcactggaac tggttgatcc ctccagtgct
      841 ccagatattg aatggcctac gcctccggca gttcaggcca gatgacattc ggcgcggtgc
      901 tggtatctgt tgccgtcacc gcgtcaatgt aatccagcac agcgttaagc tgggttgttt
      961 ctgcctgcgt cagtttccgt ccggcctgca atttcaactg aatcagactg atggaggcca
     1021 tcgcagcatc aatcagtgac tggcgctgtg cttctgccgc ttctactgcg gcaccgtgtt
     1081 gtgcctcaga atctgtcacc catttctcac catcccattt atcatatggc gttaacggga
     1141 cgatagtggt tgtattttca gggtaatcac ccggagctgt gatttctttt gcttctcctg
     1201 tttcggtgct atagataatt tcaccgcgat ggtctggcat atattcccat gagtttaaat
     1261 ctactgaacg gcagattgca taacc
//
LOCUS       DARNVM010000319         1281 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.321, whole genome shotgun sequence.
ACCESSION   DARNVM010000319 DARNVM010000000
VERSION     DARNVM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.321"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            161..1105
                     /gene="leuO"
                     /locus_tag="SI621_004751"
     CDS             161..1105
                     /gene="leuO"
                     /locus_tag="SI621_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HEI4067353.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMGEQLVSICKR"
BASE COUNT          350 a          251 c          287 g          393 t
ORIGIN      
        1 aatggattct gtttttataa gaacccgtat ctttatgttt tcctaatttt actcattttc
       61 ctttttctta ttttatatgc atgataaatc atattctcga ggattatttc tctgcattcc
      121 aataagggaa agggagttaa gtgtgacagt ggagttaagt atgccagagg tacaaacaga
      181 tcatccagag acggcggagt taagcaagcc acagctacgc atggtcgatc tcaacttatt
      241 aaccgttttc gatgccgtga tgcaggagca aaacattacc cgtgccgctc atgttctggg
      301 aatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg aaggtgatgt ttaatgacga
      361 gctttttgtt cgttatggcc gtggtattca accgactgct cgcgcatttc aactttttgg
      421 ttcagttcgt caggcattgc aattagtaca aaatgaatta ccaggttcag gctttgagcc
      481 tgccagtagt gaacgtgtat ttcatctttg tgtctgcagc ccgttagaca gcattctgac
      541 ctcgcagatt tataatcaca ttgagcagat tgcgccaaat atacatgtta tgttcaagtc
      601 ttcattaaat cagaacactg aacatcagct gcgttatcag gaaacggagt ttgtgattag
      661 ttatgaagac ttccatcgtc ctgaatttac cagtgtgcca ttatttaaag atgaaatggt
      721 gctggtagcc agcaaaaatc atccaacaat taaaggcccg ttactgaaac atgatgttta
      781 taacgaacaa catgcggcgg tttcgctcga tcgtttcgcg tcatttagtc aaccgtggta
      841 tgacacagta gataagcaag ccagtatcgc gtatcagggc atggcaatga tgagcgtact
      901 tagcgtggtg tcgcaaacgc atctggtcgc tattgcgccg cgttggctgg cggaagagtt
      961 cgctgaatcc ttagaattac aggtattacc gctgccgtta aaacaaaaca gcagaacctg
     1021 ttatctctcc tggcatgaag ctgccgggcg cgataaaggc catcagtgga tgggagagca
     1081 attagtctca atttgcaaac gctaactgaa tgcagaatag gtcagacatc aatgtctggt
     1141 ttattctgca ttttttattg aatgtagaat tttattctga atgtgtgtgc tctctatttt
     1201 aggattaatt aaaaaaatag agaaattgct gtaagttgtg ggattcagcc gatttattat
     1261 caatttaatc ctctgtaatg g
//
LOCUS       DARNVM010000320         1262 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.322, whole genome shotgun sequence.
ACCESSION   DARNVM010000320 DARNVM010000000
VERSION     DARNVM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.322"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(4..1200)
                     /locus_tag="SI621_004752"
     CDS             complement(4..1200)
                     /locus_tag="SI621_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HEI4067354.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLIFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRHQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAVGMGTV
                     FFISTKMLHANSSSSVIISMVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLAGISRGVM
                     PSLPFL"
BASE COUNT          484 a          273 c          187 g          318 t
ORIGIN      
        1 ctcttacaga aatggtaaag aaggcataac tccccgagaa ataccggcta agaagccttt
       61 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
      121 attttttgcc tgccagtcaa tcatccatat tttcaacagt ggataagcca gaccgcccgt
      181 aatcccggat atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt
      241 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
      301 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat
      361 tatcatttta cgttgcattt ccataaattg ctgtaaattc tcaatgtcat catttgcata
      421 tacagaacta tcatacgcat ttaatatttg atcgaagata atataaccag caacagcgag
      481 aaaaggcaat aaagctaaaa atgcgagaat ggcatatttt atacaatacg tagtatctaa
      541 tttcaccttg aaacgatgta taccgaaact ggtattgctc cagagaaaac tcattaccag
      601 actatataaa gtaccattaa aaataccaat ggaaacaata ccaactattg ccatcagaac
      661 catagatata ataacactac ttgaactatt ggcatgtagc atctttgtcg agataaagaa
      721 aacagtcccc atcccaacgg ccattaaaat cggcaagaaa aaggtcaccc accagaaccc
      781 tttcatagag caattaaaac taaatcttac gccgttgaga ctggtcatta aggcctgatg
      841 acgcagtcct tttgctgcca taaatataag caaaaccaac agtaacaaag tcaaaacaca
      901 accaacaagc ggcatatctg ctgacactgt cattaagatt gcgaaataga aaaaaacaaa
      961 aataagacaa ctaacaaaaa cattaccacc ggttattcca taagaaaatc gttgtccgtt
     1021 aacttccata ttagcataaa gataacgctt acatttcatt aatgcccatg gtaaatagat
     1081 acctagcgtt ataatcgtta acaacacatt caccacacaa ataagaaaat atctctcacc
     1141 tgtaccatga aaaacaaacg aatgagacgg aacatccatt tcattaataa cttgagccat
     1201 aaaaattccc tgaattaaaa aatccattga tataatttat agatacagac agtaaaatgt
     1261 tc
//
LOCUS       DARNVM010000321         1251 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.323, whole genome shotgun sequence.
ACCESSION   DARNVM010000321 DARNVM010000000
VERSION     DARNVM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.323"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            9..746
                     /locus_tag="SI621_004753"
     CDS             9..746
                     /locus_tag="SI621_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEI4067355.1"
                     /translation="MALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDE
                     ESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNN
                     GGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCR
                     KWVKPNLTCTPQDDPQYPGMETKTASDYLPQTTWPTTQLPPGYTCAEPYLFPDINKPD
                     GPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            743..>1251
                     /locus_tag="SI621_004754"
     CDS             743..>1251
                     /locus_tag="SI621_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067356.1"
                     /translation="MKRALFFLLMIFVSFGVIANCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTMWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNK"
BASE COUNT          401 a          260 c          288 g          302 t
ORIGIN      
        1 gcctgccgct ggcgcttgtc agcacggaag gggcaacaga atggtgcgca gaatacgatg
       61 aatggggcaa cctgctgaat gaagagaacc cgcaccagct gcagcagctc atccgcctgc
      121 cgggtcagca gtatgatgag gagtcaggcc tgtattacaa ccgccaccgc tattatgacc
      181 cgctgcaggg gcggtatatc actcaggatc cgattggact gaagggggga tggaattttt
      241 atcagtatcc gttaaatccg gttcagtata tagattcaat gggactggca tctaaatatg
      301 gacacttaaa taatggcgga tatggagcga gacccaacaa accgcctacg cccgatccaa
      361 gtaagttgcc agacatagcg aaacaattaa gactgccata tcctattgac caggccagta
      421 gtgcgcctaa tgttttcaaa acattcttca gagcattaag tccttacgat tacacactgt
      481 attgcaggaa gtgggtaaaa cccaatctga cttgtacgcc gcaggatgat ccccagtatc
      541 cagggatgga gacaaagaca gcaagtgatt acctgccaca gacaacttgg ccaacaactc
      601 aattaccacc aggatatact tgtgcagaac cctatttatt cccagacatt aataaacccg
      661 atgggccagc aacagcaggg atagatgatt tgggtgaaat tttagctaaa atgaaacaga
      721 gaacatcgag aggaataaga aaatgaaaag agctttgttc tttttgctga tgatattcgt
      781 tagttttggt gttatagcta attgcgaaac acaagctaaa gatcaagatt gctttaccat
      841 tttcactaag ggtactattt tttccgcgtt tcctgtctta aataataaaa ccatgtggag
      901 atggtatcag aatgaggaca taggtgagta ttattggcaa acagaacttg gtatatgtaa
      961 aaataacaaa ttcacaccaa gcggggcgag actcttaatt cgcgtcggat cactacgcct
     1021 taatgaaaat catgcaacca aaggaacgtt gcaggagttg ctaaatacag cagaaaaaac
     1081 ggcgtttctc ggcgatcggt ttagaagtta tataagagcg ggtatctatc aaaaaaagag
     1141 cagtgatcca gctcagttgt tggcagtcct tgataattca atcatggtta aatattttaa
     1201 agatgagaag ccaacttatg ctcgaatgac agcacatctc ccgaacaaag a
//
LOCUS       DARNVM010000322         1219 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.324, whole genome shotgun sequence.
ACCESSION   DARNVM010000322 DARNVM010000000
VERSION     DARNVM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.324"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..298)
                     /locus_tag="SI621_004755"
     CDS             complement(<1..298)
                     /locus_tag="SI621_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067357.1"
                     /translation="MKLGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHI"
     gene            complement(560..>1219)
                     /locus_tag="SI621_004756"
     CDS             complement(560..>1219)
                     /locus_tag="SI621_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HEI4067358.1"
                     /translation="FGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYS
                     ARLANHKLSSGDTYDLIKTLDVCNYDSETKVLTSHHGIIRNEENECYCLGALQIPFNQ
                     MKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAIASTPVYDNYFNNTTAYRPKPEDI
                     FKCGQTLKKSKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQS
                     H"
BASE COUNT          367 a          225 c          192 g          435 t
ORIGIN      
        1 gaatatgggc acgcatgccc gatagcatac tatatttttc tgaatgattc gtttctatgg
       61 taaatgaaat tttgtcatct tgaaaagatt gttggaaacg ttctatttgc caatcatcaa
      121 ttttttccac aacttcaccg agcattgtcc tggcaatact tgatatatca tcacctatac
      181 catttgcaat atacccaaac agttctttag tgaagttagt aacatgctct tgcaccatat
      241 catttgttgc gattgtcgtt gtactgtcgt tagtgcctgc cacagttcca agtttcatct
      301 tttacctcat ataattcacc gcaaggaata catagttatc atttaatctt atgacataca
      361 cctcatgttc gacggggaaa taattatatt ttgcacaaat cattcagcct taaagcataa
      421 tatgaaattt cttatctcat agtagagaaa gtccctaaag attgtagagg cgtcatcagt
      481 acatgcctct acaatcgatt aagaaaaact atgaaatact ggcaggatta aaaaaatata
      541 ccaccgtatt catggagttt taatgagatt gaaaacaact aagccctggg acaccttttt
      601 tatcaccacg ttcgttaggt gctatcttcc aggaatttag ccccatggta taattatcaa
      661 ttttttgatg ctgatctacc cctaatccat aatggccata tttctgttta ctttttttga
      721 gcgtctgtcc acatttgaag atatcttcag gttttggtct gtaagcagta gtgttattaa
      781 aataattgtc atatacaggt gtggatgcta tagcattgct tctaattaga tggataagac
      841 gatcatccat gtgttgttta tgttttttat taaatatatt tgctaattcc tcaggatcaa
      901 ttgggtcttt catctgatta aatggaatct gcaaagcacc tagacaataa cattcatttt
      961 cttcatttct tattattccg tgatgactgg ttaaaacttt cgtttctgag tcataattac
     1021 aaacatcgag cgttttaatt aagtcatatg tatcaccgga agagagttta tgatttgcta
     1081 gtcgtgcact atagttacca ttaaaattga tttcatgatt tccagccaga acgacgacat
     1141 ttttatttat acgactatct ttattaccct ccatatttcg catagaattt agcaaagtaa
     1201 ggatatattt atcaccaaa
//
LOCUS       DARNVM010000323         1206 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.325, whole genome shotgun sequence.
ACCESSION   DARNVM010000323 DARNVM010000000
VERSION     DARNVM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.325"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>1206)
                     /locus_tag="SI621_004757"
     CDS             complement(<1..>1206)
                     /locus_tag="SI621_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HEI4067359.1"
                     /translation="SPSFMPELDGGKNKTQLIVDDIVAYLKNPSVYSLEKEGPLNYFF
                     NNCSEIEPWVLIGGGLIIIVSTSEQDPESLIVTVGDIEGKYATQIPVPPSLTTSLKDI
                     FIRPHTNADDESFTKEQKKANSKYDIVDYNCKVYFNEKPEDLVVCRHISLQYCIDSMN
                     ENTGKVPLKECYSTPEGIQQHFPYELDQQFDNLINNPPPGTCVVASDKFGEILSVFFH
                     RMEKEKLTHMAAIVKSQKHAMAVRLRIKQTPAGETEYVVSFYDPNATNTAVRYKAKNC
                     DSFGSLQSFINIELANIKWVKTEICSECVGIIPYLPREQAHLLSGIDNELQPPLSPSA
                     LYLLMQMGTYENIVLFFDKLRNSQEMTVSKVLEILAAKGPGGTYGLCMSLQHDNLNKI
                     NEYIKNLKE"
BASE COUNT          363 a          231 c          199 g          413 t
ORIGIN      
        1 aactctttta aattttttat atattcatta atcttattaa gattatcgtg ttgcagtgac
       61 atacataacc catatgttcc ccctggtcct ttagcagcaa gaatctcaag aacctttgat
      121 actgtcattt cttgggaatt tcttaactta tcgaaaaaaa gaacaatatt ttcatacgtc
      181 cccatttgca ttaatagata taatgctgat ggtgataatg gtggttgcaa ttcattgtct
      241 ataccactta ataaatgggc ctgctctcga gggagataag gaattatgcc tacacactcg
      301 gagcaaatct ctgtttttac ccattttata ttcgccaact caatatttat aaatgattgt
      361 aatgacccaa aagaatcaca gttttttgct ttatagcgta ctgcagtatt ggttgcatta
      421 ggatcataaa aggatacaac atattcagtt tctccggcag gcgtttgttt tatcctcaag
      481 cggacggcca tagcatgttt ttgagattta acgattgccg ccatatgcgt taacttctct
      541 ttttccattc tgtgaaagaa gacagataat atctccccga acttgtcact ggcaacgacg
      601 catgtacctg gtggtggatt gtttattaga ttatcaaatt gctgatcaag ttcgtatgga
      661 aaatgttgct gtatcccttc tggagttgaa tagcattcct ttaatggaac tttacctgta
      721 ttttcattca ttgaatctat acaatactgc agcgaaatat gcctgcacac gactaaatct
      781 tctggttttt cattaaaata aaccttacag ttataatcta caatatcata tttactatta
      841 gcttttttct gctctttagt aaaactctca tcatctgcgt tcgtatgagg acgaataaaa
      901 atatctttga gtgatgttgt aagcgagggt ggtacgggaa tctgtgtagc atactttcct
      961 tcaatatccc ccacagttac aatcagactt tcagggtctt gctcagatgt ggagacaata
     1021 ataataagtc caccaccaat taaaacccat ggttcaatct cgctacaatt attaaaaaaa
     1081 taatttaacg gaccctcttt ttcaagtgaa taaactgaag gatttttaag atacgccact
     1141 atatcgtcaa cgattaattg agttttattt tttcctccat ccaactcagg catgaaagaa
     1201 ggagat
//
LOCUS       DARNVM010000324         1200 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.326, whole genome shotgun sequence.
ACCESSION   DARNVM010000324 DARNVM010000000
VERSION     DARNVM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.326"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(2..328)
                     /locus_tag="SI621_004758"
     CDS             complement(2..328)
                     /locus_tag="SI621_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067360.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(433..1200)
                     /locus_tag="SI621_004759"
                     /pseudo
     CDS             complement(433..1200)
                     /locus_tag="SI621_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          352 a          271 c          196 g          381 t
ORIGIN      
        1 tttatttgtt tataatgcgc ttacttaata caaaccattg aatataccct cctgacagaa
       61 aataaagcca tattattatc gccataatat acttactatc aatccttacg ccaataatgt
      121 ctgaaacagt ccctataaat attagagcaa taaagaatac tatgcctgtt ggaaaacaga
      181 ttaccatcat tccataagac atgagactaa ttaactcgct cacatcacga gtggagtcta
      241 atagtgaaag tctatgcatg gaaaagaata gtattaaaag cgataaagcc agccatatag
      301 ctctgaataa aaaaataata tatttcatta catcactccg gtgaatacaa atctaataaa
      361 gcgtccaatg ttatttccgg agcacttcat cttacatgtt tctaaagaat cattagacca
      421 gtcagggcaa gattacgaaa gcccgctccc cgcaaggact gacgcgagat agtttctgtc
      481 catggctgct tttcgcatct tacgtcttaa ccctaccttg aataccttat cattcgtcaa
      541 aatattaata gcaatgtgcc gtatccctga aaataattct gctgcgtttc ctcttcttat
      601 tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacgtg
      661 ccagtggttt cggattgctg tggcgaactt ctcagcggtt aaatcagcag agctgatata
      721 atatctgacc gtcatttctg gctctttctt ttgttctgct attattgacc gaaaggagac
      781 tgccacgtat aatttttcag tcctttccat tcaaatgtaa aatcaataag ttcatcaggg
      841 acatcgcaaa caatatgaag acggatttct tctctgccgt gactcttttc actaattgcg
      901 taactgtcat gctctggatt aattaattct ttcagcggaa atgtttcctc aaaggcttta
      961 ttcagccgcc cctggtttcc ttttacagcg aataagtaat caccaccctg tttttgtatc
     1021 ttctctgcaa tatctttctg gcaacccatc gcatcagttg tgatgatttt ctctttaata
     1081 tacaaaatgt taagaagttc agggatagct gtgatctcat tagatttctc atccgtcctg
     1141 atctgcccca ggaccagaat gtgcattgtt gagaacgcac taatgacatg aatcgctccc
//
LOCUS       DARNVM010000325         1189 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.327, whole genome shotgun sequence.
ACCESSION   DARNVM010000325 DARNVM010000000
VERSION     DARNVM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.327"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1189
                     /locus_tag="SI621_004760"
     CDS             <1..>1189
                     /locus_tag="SI621_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEI4067361.1"
                     /translation="PVYAFYETAKLYYLQQTFKPSEKIGDAGKSFLKTSVFITPLQNN
                     NNFKGRNVIVIFAEGFSSEIIGNSKMAHFPITPNLDKLSTEALSFKNYYNHTAATFRG
                     LRGQLTSGYQFRDGLTDAGTGIAQLSNVEINNIYLHRQTSLPDILKEYGYKSYFIAST
                     EKNSPLNTMLKTLNFDKVLGMGDFIGYQRDRMTDKQTFNALQYFLRSQENKNERFFIG
                     VYPSGTHHGQDSPNEKYFDGSNPLYNKFYNYDFQLGKFVDFFRRSSFYGNTLLIITSD
                     HSTFPSTEYKKSFNSDSRYFVDKIPFLIIGKNITPEVLDAGGENSLSFAPTILHMLGI
                     QYSMNYFLGCSLLDKTCTSPFSHLSAIGSDYFNIGKENHVELIQNNSGNELIKLAERF
                     YNIS"
BASE COUNT          388 a          187 c          204 g          410 t
ORIGIN      
        1 accggtatat gctttttatg aaacagcaaa attgtactat ttacagcaaa cctttaagcc
       61 tagtgagaag atcggtgatg ccgggaaatc ttttttaaaa acaagtgttt ttattacccc
      121 tctacagaat aacaacaact tcaaagggcg taatgttatt gtaatctttg ctgagggatt
      181 ttcatcggag attattggta attcaaaaat ggcgcatttt ccaattacgc ctaatttaga
      241 taagttatct actgaagcac taagttttaa aaattattat aaccatacag cagcaacctt
      301 tcgaggttta agaggtcagt taacttctgg ctatcaattt cgtgatgggt taactgatgc
      361 tggtactggt attgcacaat tatctaacgt agaaattaat aatatttatc ttcatcgtca
      421 aacatctttg ccggatatat tgaaagagta tggctataaa agttatttta tagcgtccac
      481 agaaaaaaat agcccactca acactatgtt gaaaacgctt aattttgata aagttcttgg
      541 tatgggagat tttattggtt atcaacgcga cagaatgact gataagcaaa catttaatgc
      601 actgcaatat tttttacgtt ctcaagaaaa taaaaacgaa cgctttttta ttggtgtgta
      661 cccatctggt actcatcatg gtcaagatag tccaaatgaa aaatattttg atggtagcaa
      721 tcctctctac aataaattct ataactatga ttttcagttg gggaagtttg ttgatttttt
      781 tcggcgcagt tcattttatg gcaatacgtt gttgataatt acatcggatc attctacatt
      841 tccttcgaca gaatataaga aatcatttaa cagcgattct cgttattttg tggacaaaat
      901 accatttctg atcattggaa aaaatattac gcctgaagtt ttagatgcag gcggtgaaaa
      961 ttctttaagt ttcgcgccaa caatattgca catgttagga attcagtatt caatgaatta
     1021 ttttttgggg tgttctcttc ttgataagac gtgcacatca ccattctcac atctttctgc
     1081 tattggtagt gattatttca atataggcaa agaaaatcat gttgaactaa ttcaaaataa
     1141 tagtggtaat gaattaataa aattagccga acgtttttat aacattagt
//
LOCUS       DARNVM010000326         1186 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.328, whole genome shotgun sequence.
ACCESSION   DARNVM010000326 DARNVM010000000
VERSION     DARNVM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.328"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1186
                     /locus_tag="SI621_004761"
     CDS             <1..>1186
                     /locus_tag="SI621_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEI4067362.1"
                     /translation="MSGDSLNRQRRLIAEWLKVNSDYYLDTITYEDLGLSAFKGKHAQ
                     SGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLKLILNHGIDVITLCDN
                     TVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIMTASCP
                     RWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGKESAWG
                     PSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLAPFGIS
                     NSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRLHRCDRPSIKRDLV
                     DYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLIAALSVAPEVTAIAEK
                     IRV"
BASE COUNT          400 a          174 c          262 g          350 t
ORIGIN      
        1 gatgtctggc gactcattaa accgacagag aagacttatt gctgaatggt taaaggtaaa
       61 tagtgattat tatcttgata ccataacata tgaagattta ggattaagtg cattcaaagg
      121 aaagcatgca caatcaggag ctttttcgga atttttagat gctatagagc atggttatat
      181 attgccagga actacattgt tagttgaaag tctggacaga ctttcaagag aaaaagtcgg
      241 tgaagcgatt gagcgtctga aattgatttt gaatcacggt attgatgtta taactctttg
      301 cgataataca gtctataata ttgactcttt gaatgatcca tattcattaa taaaagccat
      361 acttatagca caaagggcaa atgaagaaag cgagataaag tcaagtcggg ttaaattatc
      421 atggaagaaa aaacggcagg atgcactgga gtcaggcacg attatgacgg cgtcttgtcc
      481 gagatggctc tccttagatg acaaaagaac ggcttttgtt ccagaccccg acagggtgaa
      541 aactattgag ctaattttta aactcaggat ggaaaggcgc tcattgaatg caatagccaa
      601 gtatttaaat gatcatgctg taaagaattt ctcaggaaaa gaaagtgcat ggggaccttc
      661 tgtaattgaa aaattattag cgaataaagc tctgataggt atatgcgtac cttcatatcg
      721 tgcaagaggt aaaggaataa gtgaaatcgc tggctattat cccagagtca tatcagatga
      781 tttgttttac gctgtgcagg aaattcggtt ggcacctttt ggtattagca atagtagcaa
      841 aaatcctatg ttgataaatc tacttcgaac agttatgaag tgcgaggctt gtggtaatac
      901 catgattgtt catgcggtat ctggaagttt gcatggctat tatgtttgtc cgatgagaag
      961 actgcatcga tgtgacaggc catcaataaa gagagatttg gttgattata atatcattaa
     1021 tgagttgctt tttaattgta gtaaaatcca accagttgaa aacaagaaag atgctaatga
     1081 aactttagag ttgaaaatta ttgagctcca gatgaaaatt aataatttaa ttgctgcatt
     1141 atctgttgcg cctgaagtta ccgctatagc agaaaaaatc agagta
//
LOCUS       DARNVM010000327         1175 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.329, whole genome shotgun sequence.
ACCESSION   DARNVM010000327 DARNVM010000000
VERSION     DARNVM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.329"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(19..234)
                     /gene="essD"
                     /locus_tag="SI621_004762"
     CDS             complement(19..234)
                     /gene="essD"
                     /locus_tag="SI621_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HEI4067363.1"
                     /translation="MKSMDKLTTGIAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(424..1155)
                     /locus_tag="SI621_004763"
     CDS             complement(424..1155)
                     /locus_tag="SI621_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEI4067364.1"
                     /translation="MPYICSIILVLNSFDVRIGKEDILFKKGSAVLIDYNLKDFFSPN
                     IDHVMIVDVEEKTVNDFFKSNTLSPFSVRRFYPAYLMVECEDFSLLKNLIACLNCDGR
                     TVDVVRNQISLACLAILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAAWRLCDISSR
                     LYLSESLLKRKLKHEGLSFSKLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFV
                     SVFRRYFGVPPHQYSSRFFLEKEMM"
BASE COUNT          401 a          250 c          178 g          346 t
ORIGIN      
        1 tagttttgag tcattgaatt actctccccg tgcagccttg cgcttatctt ctttaatctt
       61 gaaataaagg tttgtcaggt acgtcagcag gccaaatacc agactaccca gcacaccgat
      121 tgcagcccac tgtgacggag ttactctatc gagcaactgt aaaaaccagt agccagcact
      181 gcctgcggag gtgccgtagg caatgcccgt tgttaacttg tccatggatt tcatagcctc
      241 acctccgcaa ataacggatg gtgtacacgg ttcggaacga agagggaaag gtatagaagt
      301 tacattagcg taaggcttga acatctattc aaaaagaaaa acgccagcga ttattctggc
      361 gtagctgaaa gcatcataca attatcaaat acgaaaatca caaaatcatt aaaacgcatc
      421 acgttacatc atttcttttt ctaaaaaaaa tcttgatgaa tattgatggg gaggaacacc
      481 aaaatacctt ctgaaaacac ttacaaaata tgacgtgttt tcataaccgc atatctcagc
      541 aacttttcca acagaatata aattgtagct taataacctt tccgccatca ccattcgctc
      601 ttcaagaatt aatttactaa atgataagcc ttcgtgcttt aattttcttt ttaacagact
      661 ttcactcaga tacagccttg aagatatatc acaaagtctc catgctgcag atatatccgt
      721 gtgaataata gccttaactt tacttcctaa actattaaga catccaaata aaaaactttg
      781 cactattttc tctgaagata agatagcaag acatgcaagt gatatttgat ttctaacaac
      841 atccacagtt ctgccatcac aattcaagca tgcaatcaag ttctttaaca atgaaaaatc
      901 ttcacattcc accatcaagt atgccggata aaaccttctt acagaaaaag gtgagagtgt
      961 gttgctttta aagaaatcat taactgtttt ctcttcaaca tctacgatca ttacatgatc
     1021 tatatttggt gaaaaaaaat cttttaaatt gtaatcaatg agaacagcac ttcctttttt
     1081 aaacaaaata tcttctttac caattcggac atcaaacgag ttcaacacca aaatgataga
     1141 acatatgtat ggcatattat ccacctgata tcatt
//
LOCUS       DARNVM010000328         1146 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.330, whole genome shotgun sequence.
ACCESSION   DARNVM010000328 DARNVM010000000
VERSION     DARNVM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.330"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1146
                     /gene="bluF"
                     /locus_tag="SI621_004764"
     CDS             <1..>1146
                     /gene="bluF"
                     /locus_tag="SI621_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HEI4067365.1"
                     /translation="IYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQ
                     LLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQ
                     AVFDKGTSKFQLTYDDRALQFFRTFVLATEQAAYFEIPAEDSWLFIADGSDKELDSCT
                     LSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTADLKSKA
                     LAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEV
                     ISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSG
                     PRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLF"
BASE COUNT          321 a          243 c          261 g          321 t
ORIGIN      
        1 ttatttatcg tagccatata cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt
       61 cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg gatcttactg tttaatggat
      121 ctcatttttt ccagcttctg gaaggtccgg aagaacaggt taaaatgata tatcgggcta
      181 tatgccagga tccacggcac tataatattg ttgagctgct gtgcgattac gcgcctgctc
      241 gccgttttgg caaagcggga atggaattat ttgatttgcg cctgcacgag cgagatgacg
      301 ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat gatgacagag
      361 cgctacaatt ttttcgtact tttgtccttg caaccgaaca agcagcctat ttcgagatcc
      421 ctgccgaaga ctcctggctt tttatcgctg acggatctga taaagaactt gattcctgta
      481 ccctttcacc aactataaac gaccactttg cctttcatcc tattgtcgat cccttatcgc
      541 ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agacagcccg tcagctatag
      601 cggttgggca gcgtaaagaa ggggaaatct atacagcgga tctcaaaagt aaggcgcttg
      661 cattcgcgat ggcacacgca cttgacctcg gtgataaaat gatttcaatc aatctattac
      721 ctatgaccct ggttaacgaa cctgacgcag tctctttttt acttgatgaa ataaaggcca
      781 atgctctggt gcctgaacaa atcatcgttg aatttactga aagtgaagtc atatctcggt
      841 ttgatgagtt tgcagaagcg attaaatcgc ttaaggctgc cggtatcagt gtagcaattg
      901 atcattttgg cgcaggtttt gccggtttgt tactcctgtc acgcttccag cctgacagaa
      961 ttaaaatcag tcaggaattg attaccaatg ttcataaaag cgggccacgg caggcaatta
     1021 ttcaagcgat cataaaatgc tgtacatcac ttgaaattca agtcagtgct atgggcgtgg
     1081 caacaccaga agagtggatg tggcttgaat ccgcaggaat tgagatgttt cagggagatc
     1141 tgtttg
//
LOCUS       DARNVM010000329         1140 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.331, whole genome shotgun sequence.
ACCESSION   DARNVM010000329 DARNVM010000000
VERSION     DARNVM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.331"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            30..752
                     /gene="sdiA"
                     /locus_tag="SI621_004765"
     CDS             30..752
                     /gene="sdiA"
                     /locus_tag="SI621_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HEI4067366.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(819..1043)
                     /locus_tag="SI621_004766"
     CDS             complement(819..1043)
                     /locus_tag="SI621_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HEI4067367.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
BASE COUNT          295 a          252 c          277 g          316 t
ORIGIN      
        1 actcactctc aggggcgttg cggtttacta tgcaggatac ggattttttc agctggcgtc
       61 gcacgatgct gttgcgtttt cagaggatgg aggccgcaga agaggtctac catgaaatag
      121 agcttcaggc tcagcagctg gagtacgatt actattcgtt atgtgttcgc cacccggtac
      181 cattcacgcg acctaaagtg gctttctaca ccaattaccc tgaggcttgg gttagttatt
      241 atcaggcaaa aaactttctc gcaattgatc ctgtgctgaa ccctgaaaac tttagtcagg
      301 gccatttaat gtggaatgat gacttattca gcgaagcaca gccgttatgg gaagccgcgc
      361 gcgcacatgg tttacgccgc ggtgtcactc agtatttaat gctgccaaat cgggcgctgg
      421 gctttttgtc cttttcccgt tgcagcacgc gcgaaatacc cattcttagt gatgaactgc
      481 aattaaaaat gcagttactg gtgcgagaaa gtctgatggc tctgatgcgt ttaaatgatg
      541 aaatagtgat gacgccagag atgaatttca gcaagcgcga aaaagaaatt ctgaaatgga
      601 cggcggaagg gaagacatca gcagagatag cgatgatttt gtcaatctct gagaatacgg
      661 tcaatttcca tcagaaaaac atgcagaaaa aaatcaatgc accaaataag acccaggtgg
      721 cctgttacgc ggccgctact ggcttaattt gatcgctttt ctttctgcgt gccagatgca
      781 aaaaccggct gaaaggcacg ctctcagccg gttttatatt actgacggta ggcttgttta
      841 atttgcttaa ccgttttgga aaatactgca gcctgggttt cgtcttcgat cagcgcaagc
      901 tgtttttcca tctttaacat cacgcggccc gcgtcagctt gtcccatcgc ctgcagcatc
      961 agcgtcagca tcgctttcag gcaggagact tcatttgcca gttcttgatt attctcggca
     1021 gtagaaaaat caggcgtact catttatttt cctcgtcatg ttgcaatgaa aatttgcggt
     1081 gaaaaatgtt aaggcggcgg agtataccat aagctttgct aaaaatagca gtggttgttt
//
LOCUS       DARNVM010000330         1140 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.332, whole genome shotgun sequence.
ACCESSION   DARNVM010000330 DARNVM010000000
VERSION     DARNVM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.332"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..1118
                     /locus_tag="SI621_004767"
     CDS             <1..1118
                     /locus_tag="SI621_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067368.1"
                     /translation="SNINNTAHNKNLDKTTFDEQPAYETRVSQAKKILQSFAPNDIKV
                     DTNTKFISLIESVRALSPELQCKVLDGLNYAKNTLHNINQNSAIATKSSCQSTESIEK
                     TITFNILDMLDNLTMRQQNIMEAFLRVFTSMYNASAKADASMSIVFEQLVHLQSSETK
                     NASWANFAGALAGSAINATGAVFMAGAELAKPAGKIDDIAENQEQNTDKPDSQTRIKS
                     KEDMANAKARLAEIENQQKDLSNNKNIEDNDKQEQSSKLQKEKESITNKIKDKEKINS
                     HNLRYGITSSISHTNNLFQSIANLSQYGGTVAAEHTRAEKDLAEQAKNVMHSVMSSYL
                     DQINRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
BASE COUNT          450 a          198 c          196 g          296 t
ORIGIN      
        1 ttagcaatat aaacaataca gctcataata agaatctcga taaaacaaca ttcgatgaac
       61 aacctgctta tgaaacaaga gtatcacaag ctaaaaaaat actgcaaagt tttgcgccaa
      121 atgatattaa agttgataca aatactaaat ttattagtct gatagagtct gtccgtgctc
      181 tctctcctga attacaatgt aaggtactag atggtcttaa ttatgctaag aatacactac
      241 ataatataaa tcaaaattct gccatagcaa caaaatcatc ctgtcagtct accgagtcaa
      301 tagagaaaac cataacattt aatattctcg atatgttaga taatttgacg atgagacaac
      361 agaacattat ggaggccttt ctaagagtct ttacatcaat gtacaatgcc tctgccaagg
      421 ctgatgctag catgagcatc gtttttgaac agcttgtcca tcttcagagt agcgaaacaa
      481 aaaatgcttc ttgggcaaat tttgcaggtg cattagcagg ctcagctatt aatgctacgg
      541 gagctgtatt tatggcaggt gcagaacttg cgaagcccgc gggaaagata gatgatatcg
      601 cagaaaacca ggaacaaaat acagacaaac cagatagcca aactcggata aaatcaaaag
      661 aagatatggc aaatgcgaaa gccagacttg ccgaaataga aaatcaacaa aaagatttat
      721 caaataacaa gaatattgag gataatgaca aacaagagca atcaagtaag ttacaaaaag
      781 aaaaagaaag tatcactaat aaaattaaag acaaagaaaa aattaatagt cacaatctaa
      841 gatatggtat taccagttct atttctcata ccaataatct atttcagtct attgcaaatc
      901 tatctcaata cggcggaaca gtggcagcag aacatactcg tgcagaaaaa gacttggctg
      961 agcaagctaa aaatgttatg cattcagtta tgagtagcta tctggatcaa attaatcgtg
     1021 atgttcaagg aagagatcaa gcactcaatt tattacagca aataatacaa accattattg
     1081 atactgatag tgcaatcgtt caaggcattc gctcataaat tattgcaata atagttagtt
//
LOCUS       DARNVM010000331         1127 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.333, whole genome shotgun sequence.
ACCESSION   DARNVM010000331 DARNVM010000000
VERSION     DARNVM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.333"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(165..722)
                     /gene="rfbC"
                     /locus_tag="SI621_004768"
     CDS             complement(165..722)
                     /gene="rfbC"
                     /locus_tag="SI621_004768"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEI4067369.1"
                     /translation="MNIVKTDIPDLIVLEPKVFSDERGFFMESYNQIEFEKTIGRHVN
                     FVQDNHSKSSKGVLRGLHYQLAPYAQAKLVRCVVGQVFDVAVDLRKNSPTFKKWFGIT
                     LSAENKRQLWIPEGFAHGFLVTSDEAEFIYKTTNYYAPGHQQAIIYNDPILNIDWPFC
                     SSALSLSQKDQEAKLFSELLDSELF"
     gene            complement(743..>1127)
                     /locus_tag="SI621_004769"
     CDS             complement(743..>1127)
                     /locus_tag="SI621_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067370.1"
                     /translation="ERLIAAGFQVNFFPITSDLELASFYRSNDIMIAAGLIEDGAFHY
                     PCAESMACGCLVISNYAPLTETNSVLKLVKFDACKLGEAINLCLNLDLEEKSKEIQSN
                     ISVLNKYDWKIVGETFNSLLLDANK"
BASE COUNT          388 a          217 c          167 g          355 t
ORIGIN      
        1 cgataatgaa gcctacaata agtgtaaata taaaaaataa gtagataaaa cttattaagc
       61 ttttatcgtg tttatattta ggactatttt ctgtcattat acaaacatac tcaaaaaata
      121 taagcaacca cctatccttc agacggataa ggtggcacac tttattagaa cagttcactg
      181 tccaataatt ctgaaaataa ttttgcttct tgatcttttt gcgataatga cagagcacta
      241 ctgcagaaag gccaatcgat gtttaaaata ggatcattgt aaatgattgc ttgctgatga
      301 ccaggagcat agtagttcgt tgttttataa atgaactcag cttcatcact ggtcaccaag
      361 aaaccatgag caaatccttc aggtatccat aattgtcgtt tattttctgc ggaaagggtt
      421 attccaaacc attttttgaa cgttggtgaa ttttttctaa gatcaacagc aacatcaaat
      481 acctgaccta caacacatcg aactaattta gcctgtgcat acggtgctaa ttgataatgt
      541 aacccacgta gtacgccttt actagatttt gaatgattat cctgaacaaa atttacgtgc
      601 cttcctattg tcttctcaaa ttcaatctga ttataactct ccataaaaaa gccgcgttca
      661 tcactaaaca cttttggttc aagaacgatc agatctggaa tatcagtttt aacaatattc
      721 atattttccc catcaacgta tactatttat ttgcatctaa caataaacta ttgaaagttt
      781 caccaacaat tttccagtca tatttattca acacagaaat attagattgg atttctttgc
      841 ttttttcttc taggtcaaga ttgagacaaa gattaattgc ttcaccaagt ttgcaagcat
      901 caaacttgac taatttaagt acactgttag tttcagtaag tggcgcataa tttgaaataa
      961 caagacaacc acaagccatt gattcagcac aaggataatg gaaagcgcca tcttcaatta
     1021 acccagctgc aatcatgatg tcattgcttc gataaaagga tgccaattct aaatcagaag
     1081 taatcggaaa aaaattaacc tgaaacccgg cagcaattaa acgctcc
//
LOCUS       DARNVM010000332         1102 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.334, whole genome shotgun sequence.
ACCESSION   DARNVM010000332 DARNVM010000000
VERSION     DARNVM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.334"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(1..1079)
                     /locus_tag="SI621_004770"
                     /pseudo
     CDS             complement(1..1079)
                     /locus_tag="SI621_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041204891.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          297 a          250 c          177 g          378 t
ORIGIN      
        1 ctgtccatgg ctgcttttcg catcttacgt cttaatcctg ccttgaatac cttatcattc
       61 gtcaaaatat taatagcaat gtgccgtatc cctgaaaata attctgctgc atttcctctt
      121 cttattttac aatcgtcttc attcattact acgtccagac gccagtgcag cttattctcc
      181 acatgccagt gatttcgaat cgctgtcgca aacttctcag cggttaaatc agcagaactg
      241 atataatatc taaccatcat ttcgggctct ttcttttgct ctgctattat tgagcgaaag
      301 gagactgcca cgcataattt tttcagtcct ttccattcaa acgtgaaatc aataagttca
      361 tcagggtcat cgcaaacaat atgaagacgg atttcttctc tgctgtgact cttttcacta
      421 attgcataac tgtcatgctc tggattattt aattctttca gtgggaattt ttcttcaaaa
      481 gctttattaa gccgacgctg atttcctttt acagcgaata agtaatcacc tccctgtttt
      541 tgtatcttct ctgcaatatc tttctggcaa cccatcgcat cagttgtgat aatttttcct
      601 ttaatatcca acatgttaag aagttcaggg atagctgtga tctcattaga tttctcatct
      661 gtcttaatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
      721 gctcccttgc ggcgactctt gtcataagaa tgccggagcg tttttccatc aattgcaatg
      781 acgtctttat catttgatga atggcagtca cgcatccagt taataaagca ctcgtgaaat
      841 tttgcaggac tgatacagga tataactctg gcaatggtat cgtgaacagg aataccattt
      901 tcaaaatcac catattgctt caaaaaatcg agatgtgttt ccccaaaatc ctctatatct
      961 tcccatcctt ctgcaccaga aataacggca caaatagtca acagtagaat atccgataat
     1021 ttatgttcca ctttctaggc ttgtctgtaa tcgggaataa tagaatatgt tccatcaatt
     1081 ttttaagttc cattttgctc tc
//
LOCUS       DARNVM010000333         1072 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.335, whole genome shotgun sequence.
ACCESSION   DARNVM010000333 DARNVM010000000
VERSION     DARNVM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.335"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            22..624
                     /locus_tag="SI621_004771"
     CDS             22..624
                     /locus_tag="SI621_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEI4067371.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(663..>1072)
                     /gene="nudI"
                     /locus_tag="SI621_004772"
     CDS             complement(663..>1072)
                     /gene="nudI"
                     /locus_tag="SI621_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HEI4067372.1"
                     /translation="VCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEAL
                     RREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKIN
                     EEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          284 a          249 c          234 g          305 t
ORIGIN      
        1 ttgtctatag gtggagttta cgtgttagct ttttgccgct cttcgttgaa gtcaaaaaaa
       61 tatttcatca ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg
      121 agtagcaatg gtttgccacg tatcgacaat aaaacgctgg ccagactggc acagcagcat
      181 ccggttgtcg ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg
      241 tcagataaaa caggtattac ggtcaaaggt acccaggatg cccgtgaact gggcaacgct
      301 tttagtgctg atatccctga tttcgatctt tattccagta ataccgtccg gaccattcag
      361 tcggcgacct ggttttcagc gggtaaaaag ctgacggtag ataaacgact tcttcagtgc
      421 ggtaatgaga tttatagtgc aattaaggac ttacaaagca aagcgcctga taaaaatatc
      481 gttattttca cccataatca ttgcctgaca tatattgcta aagataagcg tgatgcgaca
      541 tttaaacctg attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg
      601 gatggggaat tcgttaacca ctaaattttc aatctgacag ccagtaatgg ctgtcatcat
      661 tattacagaa gacctttcaa acgtaacgtt tttcgggtgg cgacattcaa atcatagtgc
      721 gccagatctt caggttttac ccacgcgtag tcctgaaact cttcgtttat tttcacttct
      781 cggttggcag aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca
      841 tctgcatacg tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa
      901 agcagctgtt ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg
      961 ccaggctcca cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg
     1021 gccattttac acagcaaata agcaccatca ttttgaatca aagggcatac aa
//
LOCUS       DARNVM010000334         1054 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.336, whole genome shotgun sequence.
ACCESSION   DARNVM010000334 DARNVM010000000
VERSION     DARNVM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.336"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            40..378
                     /locus_tag="SI621_004773"
     CDS             40..378
                     /locus_tag="SI621_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067373.1"
                     /translation="MPFRIQTYSPVTKSKIENFAIKDGAQKLLQINREKLLMLLNRSS
                     NECYHFDDKNVRIYIIDGKDRYYIDKNGVVSYGVQDNVICSNNKINEDSIEKILLEGN
                     IDFWKKLKIK"
     gene            831..>1054
                     /locus_tag="SI621_004774"
     CDS             831..>1054
                     /locus_tag="SI621_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067374.1"
                     /translation="MADKAILWALISASTQEGRKACSLSYFSCKAAEAELGLAYMAAN
                     DNKAFLTSLSRIMMYKIDAGLSESYTCYLL"
BASE COUNT          371 a          162 c          207 g          314 t
ORIGIN      
        1 ttttcatctt gctgtatagc tgatgttttc cattatagga tgccttttcg catccaaaca
       61 tattcccctg tcactaaaag taaaattgaa aattttgcaa taaaagatgg tgcacaaaaa
      121 ttattacaga taaatcgtga gaagttatta atgttactga acaggtctag taatgagtgt
      181 taccattttg atgataaaaa tgttaggatt tatattattg atggtaaaga cagatattat
      241 attgataaaa acggggtggt gtcatatggc gttcaagata atgtcatatg ctctaataat
      301 aaaattaatg aagattccat agaaaaaatt ttattagaag ggaatattga cttttggaaa
      361 aaactgaaaa ttaagtgaaa tgaacgaaag agaaaattaa caatgagcgg aaaaccagcg
      421 gtatactaag gcgatgccat gttgaaagat agtccaatag tctaaagtgg agaactcaat
      481 gaagcaaacc ggacgaggct tgcttaacag tcctggagca accaatagaa aacctaatgg
      541 tatacgttta gtaccgtcag caaatacaga aacgtataat cgtgatggat ttttaacaca
      601 tagttgttta aacgcttttg gtccatcttt aggacctcga ttctgttccg agggttgtat
      661 aactggttca tctaatgaca tgcaaaaatt aaatgagcta atattttcag agccagatag
      721 cacattaaca gtgacggact agaaatatga caaggttatt aacaattgtg cttttcttct
      781 ctatttctgc gaatgcagtt actgtaaacc aatttcagag agagttggat ttggcagaca
      841 aggcaatatt atgggcacta atatcagcca gtacccaaga gggacgaaaa gcgtgttcac
      901 ttagctattt ttcatgtaaa gccgcagagg ctgaactcgg actggcatat atggctgcta
      961 atgataacaa ggcatttctc acatccttat ctagaataat gatgtacaaa atagatgctg
     1021 gactttccga atcctataca tgctatttat taag
//
LOCUS       DARNVM010000335         1024 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.337, whole genome shotgun sequence.
ACCESSION   DARNVM010000335 DARNVM010000000
VERSION     DARNVM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.337"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>1024)
                     /locus_tag="SI621_004775"
     CDS             complement(<1..>1024)
                     /locus_tag="SI621_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HEI4067375.1"
                     /translation="QLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAK
                     LDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPY
                     RVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAS
                     SGGAEPSVFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGEL
                     AAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPDIRYRYDSDGRVTEQLNPAGLSYTY
                     QYEKDRITITDSLDRREVLHTQGEGGLKRMVKKEHADGSVTQSQFDAVGGLKAQTDAA
                     GRTTEY"
BASE COUNT          169 a          358 c          293 g          204 t
ORIGIN      
        1 ctgtactcag tcgtccggcc tgcggcatcc gtctgtgcct taagcccgcc caccgcgtca
       61 aactgactct gcgtgacgct gccgtccgcg tgttcctttt tcaccatccg cttcagcccg
      121 ccttcgccct gcgtgtgcag cacttcacgg cggtccagac tgtcggtgat ggtgatgcgg
      181 tctttctcat actgatacgt gtagcttaag cctgccgggt ttagctgttc tgtcacccgc
      241 ccgtcgctgt cgtaacggta acggatatcc ggccggcccg tgtgacggtg cgccaccatc
      301 cggccccggt atttatcatc gtaagtaaag ctgcgcacct gtgtattgct gcggtcatac
      361 accgccgcca gttcgccgcg tggcgtccag ccatagcgca ccagcggcgc ggcaggtaaa
      421 ttctccgggt attccgggtc gtgtgtcagc cacacggcag acagacggat gccgttgtcc
      481 cggccatatt cggtgtaacc cggcagggta tcaggaaaaa cggacggttc cgccccgccg
      541 gaagaggcct gctgccgggc ctcttctgcc cgctgcgcct gcgtggtcag taccagccgg
      601 aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc accgccagcc
      661 tcacggtgaa acgtctgtgt gcgcccgaag cggtccacca gcccggtcag tacccggtac
      721 ggcggcagcg gcgcaggcag cacctcatcc gcctccggca cccgctcaca ccagccgagt
      781 acccaccacg gcccctgcgg actgttcgtc gccagataac gatgcggact caggcgaagc
      841 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccacg
      901 ccgccgcgca ccagccagag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg
      961 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
     1021 tgta
//
LOCUS       DARNVM010000336         1017 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.338, whole genome shotgun sequence.
ACCESSION   DARNVM010000336 DARNVM010000000
VERSION     DARNVM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.338"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>1017
                     /locus_tag="SI621_004776"
     CDS             <1..>1017
                     /locus_tag="SI621_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4067376.1"
                     /translation="QLQQFIKIMEQKAQLPIYIDKKNLKESIFSVLHNDPQHVDKDQH
                     LPCDKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIQDNNNKTETFSF
                     TNLQWENLLQHYRENHQQPNIAGSRNITDNRDKIKNTISTSEIIECASPEIISSVLND
                     LYSIANFLPDNNLTPNESWKRFCNTCERFYVAQKSITGDKSERLTRKLSISDAGITMT
                     FTIGDVVINTISTAIPEDSTGQRCIEGLNLAGMDLTGIDLSKMVLRNVNFNGSILRNA
                     KFSGTICEGVDFTDCDLRNAEFENASLEKIDFRKARHLLNINFTNANLRNSNFSGKVL
                     TGVN"
BASE COUNT          360 a          194 c          182 g          281 t
ORIGIN      
        1 agcaactgca gcagtttatt aaaataatgg aacaaaaagc gcaactacca atctatattg
       61 acaagaaaaa tttgaaagag agtattttct ctgttttgca caatgaccca caacacgtag
      121 ataaagatca acaccttccc tgtgataagt ttttaaaaca tgcctgcaaa agttcaaatt
      181 catttgaagt gaaattagat gccactcatc aatatcaaca cctgaataac ttcatgattt
      241 cttttgaccc agtagaaaat caattaacaa tacaggataa caataacaag actgaaactt
      301 tctcgtttac aaacttacaa tgggaaaatt tgctgcaaca ctatagagaa aaccaccagc
      361 agccaaatat agcaggatca cgaaatatca cggataatag agataaaatt aaaaatacaa
      421 tatccacctc tgaaattatt gagtgcgcct ctcctgaaat aataagtagc gtcctgaacg
      481 atctttatag cattgctaat ttcctcccgg acaataatct gaccccaaat gagagctgga
      541 aaagattttg taacacatgc gagcgctttt acgttgctca gaagagtatc actggagata
      601 agagtgaacg tcttacacga aaactctcta tctctgatgc aggaattaca atgaccttca
      661 cgataggtga tgttgtcatc aatactatta gcactgctat tcctgaagat tcaacggggc
      721 aacggtgtat cgaagggttg aatttagcgg gaatggattt aaccggcata gacttgtcta
      781 aaatggttct aaggaatgtc aattttaatg gcagcattct tagaaatgcc aagttctccg
      841 gtacgatctg tgaaggcgtg gattttaccg attgtgatct gcgtaatgca gaattcgaaa
      901 atgcctcatt agaaaaaatc gattttcgta aagctcgtca cttgcttaat ataaatttta
      961 caaatgcaaa tctacggaac agtaacttca gcggaaaagt tctcactggc gttaatt
//
LOCUS       DARNVM010000337         1012 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.339, whole genome shotgun sequence.
ACCESSION   DARNVM010000337 DARNVM010000000
VERSION     DARNVM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.339"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            2..955
                     /gene="ompT"
                     /locus_tag="SI621_004777"
     CDS             2..955
                     /gene="ompT"
                     /locus_tag="SI621_004777"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HEI4067377.1"
                     /translation="MRAKLLGIFLTPPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          319 a          216 c          227 g          250 t
ORIGIN      
        1 tatgcgggcg aaacttctgg gaatattcct gacaccccct attgcgatca gctcttttgc
       61 ttctaccgag actttatcgt ttactcctga caacataaat gcggacatta gtcttggaac
      121 tctgagcgga aaaacaaaag agcgtgttta tctagccgaa gaaggaggcc gaaaagtcag
      181 tcaactcgac tggaaattca ataacgctgc aattattaaa ggtgcaatta attgggattt
      241 gatgccccag atatctattg gggctgcggg ctggactact ctcggcagcc gaggtggcaa
      301 tatggtcgat caggactgga tggattccag taaccccgga acctggacag atgaaagtag
      361 gcaccctgat acacaactca attatgccaa cgaatttgat ctgaatatca aaggctggct
      421 cctcaacgaa cccaattacc gcctgggact catggccgga tatcaggaaa gccgttatag
      481 ctttacagcc agaggtggtt cctatatcta cagttctgag gagggattca gagatgatat
      541 cggctccttc ccgaatggag aaagagcaat cggctacaaa caacgtttta aaatgcccta
      601 cattggcttg actggaagtt atcgttatga agattttgag ctaggtggca catttaaata
      661 cagcggctgg gtggaagcat ctgataacga tgagcactat gacccaggaa aaagaatcac
      721 ttatcgcagt aaagtcaaag accaaaatta ctattctgtt gcagtcaatg caggttatta
      781 cgtaacacct aacgcaaaag tttatgttga aggcgcatgg aatcgggtta cgaataaaaa
      841 aggtaatact tcactttatg atcacaatga taacacttca gactacagca aaaatggtgc
      901 aggcatagaa aactataact tcatcactac tgctggtctt aagtacacat tttaagaacg
      961 ccaactaaaa tttccccgag gtgaaaatcg ccccggggaa taactagcca tt
//
LOCUS       DARNVM010000338         1006 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.340, whole genome shotgun sequence.
ACCESSION   DARNVM010000338 DARNVM010000000
VERSION     DARNVM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.340"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            309..1006
                     /locus_tag="SI621_004778"
                     /pseudo
     CDS             309..1006
                     /locus_tag="SI621_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          340 a          159 c          196 g          311 t
ORIGIN      
        1 atgcggtttc tgaaattaac ctgtcatcta ataaaaataa tgttgatcag tttattgaat
       61 gcgctaagag aaatgaacag accctattcg gcaatataag aaaaagtgat tttcatgttg
      121 cttcacttca gccaggtaga acgcgtagtg ttatttcaga aacgccgcca aatgactgta
      181 tggaatcaca taatttatat gaaaaccaca cagatacggt ttcaactgta actaaaaatt
      241 ctcagcaagt taaaggtcac tatggagata agttgaaaga aatgcagttg ttcctcaacc
      301 agatgagcaa tgcacttcaa caggattcat ctttgttaga gtcaaaggaa cacaccatag
      361 atattcaaga aaaaacgaat aagtttgtgc agcattttca gcgggtatta tttgataaaa
      421 atggaaggtc atcagagttt ctacttaatt tttatgagtg ttgctataag tttttaccaa
      481 gagcgcagcc tcaagataaa atcgatagct ataattcagc actgcaagct ttttccatct
      541 tttgttcatc tacgttgaca cataataatg tagggtttaa tttcaaatta tttccagaag
      601 tcaagctgtc tggaggagag cttgaaacgg tattcaaata caaaaatggt aattttgtct
      661 gggagatagc cagaattaaa attactctcc caaaagaaga gggtggttta tataatttac
      721 gtggattgga ttttaaggga tgcttctttt ctggacagaa cttcagtaac tatgatattc
      781 aatatgtgaa ctggggaacg tcattgtttg atcttgatac tccatgtatt tttaatgcgc
      841 ctgcttacaa caagagtaat gaaaaatcat tagaacgcgt cagcgaaaac ggtttaagtg
      901 gagtcttgtc tgatcgtaat aaaaaaataa aactaatcac gggtgtggca ccattcgatg
      961 atattttatt tatggatgat gactttgatg ataactcccc tgatga
//
LOCUS       DARNVM010000339         1000 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.341, whole genome shotgun sequence.
ACCESSION   DARNVM010000339 DARNVM010000000
VERSION     DARNVM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.341"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..293)
                     /locus_tag="SI621_004779"
     CDS             complement(<1..293)
                     /locus_tag="SI621_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067378.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPP"
     gene            complement(503..>1000)
                     /locus_tag="SI621_004780"
     CDS             complement(503..>1000)
                     /locus_tag="SI621_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEI4067379.1"
                     /translation="SITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFLHNVTVP
                     TDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTSVEGKQEH
                     YFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGTSGADDWR
                     APLEA"
BASE COUNT          232 a          272 c          270 g          226 t
ORIGIN      
        1 ggtgggcaca ccttcatgct gtacagcatc tggtttttgt cgttctcccc cagttcaaac
       61 caggtcacca cacctttcac ccggcgctgt acgtcatcgc cctgccatac cgtcaggtag
      121 gccattttgt ccagcacctg cgcaaattca agggagagaa actgctggct gaccagagag
      181 agatcgaggg aaaagagcga agagagtgac tggttcagat gaaaggagac caccgcaaac
      241 gcgtccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga catagcaacc
      301 tccgggttta aggaaaaaca gacagttcgc tgttaaagcg gctgtaacag tacagtgtta
      361 cgcttgcgtt caggcacgct accgccgaag tttccggcat ccgccatctc ccgtgaacgt
      421 aaccccttac tgacaacctt tctttttacg gcgtaataca aaaacagcat ccggcctaat
      481 agccggatgc tgttggctta acttatgctt ccagcggtgc gcgccagtcg tcggcaccgg
      541 aggtgccaga cttgacgtgt tcccactcta ttttgcggta ggccagtgaa acttcgagca
      601 actgggtgaa ctcgcgtttc gccggatcct ggcaatgcgg catatgcagt ttcatatcga
      661 caatcgtcga atcggtcagg cgggtggtga aataatgctc ctgtttgcct tccacagagg
      721 tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt gcgttgtaca
      781 tcagcggaac agctttgttc agcgccacgg taaaaatgaa cggcttgtga gcacgctgac
      841 ccgcaggctg gccggactga ggatcggtcg ggacagtaac gttatgcagg aattcctgca
      901 ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca gcggtaaatg
      961 cacctgcagt aatatttccc tgggtctgac cagtgatgga
//
LOCUS       DARNVM010000340          998 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.342, whole genome shotgun sequence.
ACCESSION   DARNVM010000340 DARNVM010000000
VERSION     DARNVM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.342"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>998
                     /locus_tag="SI621_004781"
     rRNA            <1..>998
                     /locus_tag="SI621_004781"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          265 a          211 c          324 g          198 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtggcctgt gagggttgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaa
//
LOCUS       DARNVM010000341          971 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.343, whole genome shotgun sequence.
ACCESSION   DARNVM010000341 DARNVM010000000
VERSION     DARNVM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.343"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..865
                     /gene="hypT"
                     /locus_tag="SI621_004782"
                     /gene_synonym="qseD"
     CDS             <1..865
                     /gene="hypT"
                     /locus_tag="SI621_004782"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HEI4067380.1"
                     /translation="LTLEACRNFCQAAKDRNLSQPAFSRRIKALESAIGVVLFDRTTS
                     PLQLTEEGRLFHSQTRSLVQQLECNLNELGGDNSFNLPNIKIAAAHSLSLSMLPKLLH
                     SLTTLGGDFIYHVEAIDVVQAVNTLREGKSDFIISFRDEEFMQSPFCCMKLFESELYP
                     VCAADQQGKPIFDLTNSQAPLLDYTPTSYMGRLVNRHLAEQGGIAGHTILVSSMSELL
                     KNMALNGLGIAWLPLWSIADELNTKRLVCPWPAKWAVPIQAYIYRMDTRLNKTAEHFW
                     TILRNNIKENS"
BASE COUNT          267 a          200 c          217 g          287 t
ORIGIN      
        1 cctgacactg gaagcctgtc gcaatttctg tcaggcagca aaagatcgta acctttcaca
       61 accagcattc agcagacgaa taaaagcgtt agagtcagct ataggtgttg ttttgttcga
      121 tcgtacaacc tctcctttac aattaactga agagggtagg ctttttcact cccagacacg
      181 cagtttagtg caacagcttg aatgcaatct gaatgagtta gggggggata attcattcaa
      241 tttgcctaat attaagattg cagctgcgca ctcattatcc ctcagcatgc tacctaaact
      301 tctgcactca ctcaccacct taggcggaga ctttatctat catgttgagg ctattgatgt
      361 cgtccaggcc gtaaatacgt tacgagaagg gaagagtgac tttattattt catttcgaga
      421 tgaggagttt atgcaatcgc cattttgttg tatgaaactg tttgaatctg agttgtatcc
      481 ggtatgtgct gctgatcaac agggaaaacc gattttcgat cttaccaatt cacaggctcc
      541 tttgctcgac tatactccaa cttcttatat gggcagactg gttaatcgtc atttggcaga
      601 acagggaggg atagccggac acacaatatt ggtatcttca atgagtgaac tactaaaaaa
      661 tatggcactc aatggacttg gtattgcctg gctccctctc tggtcaatcg cggatgaact
      721 gaacacaaaa cgacttgtgt gtccgtggcc tgctaaatgg gcagtgccta ttcaggctta
      781 tatttatcgt atggatactc ggctgaacaa aacggctgaa cacttttgga cgattttacg
      841 aaataacatt aaagagaata gctagaaaca tcgtggacag tttttgttga gcacgacaaa
      901 tccacgtttt tctgggggtg aaggggggga attcgttaaa ctggatgtct tttgctttaa
      961 tcagttcatc t
//
LOCUS       DARNVM010000342          945 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.344, whole genome shotgun sequence.
ACCESSION   DARNVM010000342 DARNVM010000000
VERSION     DARNVM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.344"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            13..774
                     /locus_tag="SI621_004783"
     CDS             13..774
                     /locus_tag="SI621_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067381.1"
                     /translation="MYVLFSLLAGCVSESLIDEKKKKAQTTQSNFNKNAPQQLTDKDL
                     FGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRKYYTVSTF
                     SGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIVYQDTLQTG
                     KYDTALKSTVWSDYKNEKLTNTISLRYLVRFTLVDVATGEWAAWSPVNYEYKVLPPFP
                     GKNEASTTDMTEQQIIQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          320 a          182 c          197 g          246 t
ORIGIN      
        1 aaggtttgtt gtatatatgt attattctcg ctgcttgcag gatgtgtctc tgaatctctt
       61 attgatgaaa agaagaaaaa agctcaaacc acacaaagta attttaataa aaacgctccg
      121 cagcaactaa cagacaaaga cttatttggt aatgaaacca cactagccgt atccgaggag
      181 gatattcaag ctgcgcttga tggagatgag ttccgcgttc cccttaattc acctgtaata
      241 cttgttcaat ccggcaaccg cgcaccggaa accattatgc aggaggagat gcgaaaatat
      301 tatactgttt ccacattctc tggtatcccg gacagacaaa agcctctgac ttgtaacaaa
      361 aacaaagata aaaatgagaa cgaggatgtt gccagtgctg aaaatatgaa ctggatgcag
      421 gcattacgtt ttgtggcggc aaaaggccat cagaaagcga ttattgttta ccaggatacg
      481 ttgcagacag gaaaatatga cactgcactg aaatcaacag tatggtccga ttataaaaat
      541 gaaaaactga ctaacacaat ctcactgcgc tatctggtgc gattcacgct ggtggatgta
      601 gctacaggtg agtgggctgc ctggtcgccg gtgaattatg aatataaagt gcttccacca
      661 tttcccggca agaatgaagc cagtacgact gatatgacag agcagcaaat catacaactt
      721 aagcaaaaga cctataaagc gatggtaaaa gatttggtta accgctatca ataataaatt
      781 atatctgccg ccaggaattt ctggcggcaa taatacaaaa ttctttgcat aagaaattgt
      841 tcctgacaaa gacagtgcaa gaaaggacat ggaatccggc gttaaaaact gtttgtttta
      901 gaatatttaa ttatttaact gcttaatctt ccttgcgaga aatat
//
LOCUS       DARNVM010000343          923 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.345, whole genome shotgun sequence.
ACCESSION   DARNVM010000343 DARNVM010000000
VERSION     DARNVM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.345"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(226..768)
                     /gene="fimA"
                     /locus_tag="SI621_004784"
     CDS             complement(226..768)
                     /gene="fimA"
                     /locus_tag="SI621_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HEI4067382.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          284 a          191 c          176 g          272 t
ORIGIN      
        1 catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataagaaata taaagaaatg
       61 attgctttgg tttcttacaa cacatcattc ccttttcatt taataaagcg gcatgataca
      121 aagtgaacat gatttcgatt tacattgcca ccataccgct tcagacaacc ggtacattac
      181 tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt cgtatctcat
      241 aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg tgcctttata
      301 acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg ttgagaagct
      361 attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac cgacacccgt
      421 tgctgttgtt gtattagtac tggatgcaat ggccaataaa ttatcattga ttgcatccgc
      481 ctgaccagaa aatgcaacgg cagcattagc ggcaacgaca ggatcgcagt cgttcaactg
      541 tatagtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat cggtacgata
      601 ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg cagcattcac
      661 cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac tacccgtagc
      721 ggcgaatagt gcggcggcca gcgcagacga aataaatctt aatttcatat atattccttc
      781 aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa gacactgata
      841 gtagtaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
      901 aaaaatagag agccccgtta gat
//
LOCUS       DARNVM010000344          921 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.346, whole genome shotgun sequence.
ACCESSION   DARNVM010000344 DARNVM010000000
VERSION     DARNVM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.346"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..551)
                     /locus_tag="SI621_004785"
     CDS             complement(<1..551)
                     /locus_tag="SI621_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HEI4067383.1"
                     /translation="MDNLIGTPPNYVVPHKYIDTEQMEYLCHLNRFSKSPDDFLITGQ
                     REDLFTYKYILDGSFSNLQHLFPKGQLQKIQQRLSSLMYKNMFHCFHVFLLKLCSIES
                     IPLPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHHNFS
                     TTIALTNDSFHISSINNHNAQVI"
BASE COUNT          314 a          141 c          158 g          308 t
ORIGIN      
        1 aatataacct gtgcgttatg gttgtttatt gaagatatat ggaagctatc atttgttaaa
       61 gctatggttg tgctaaaatt atggtggtga tcagcgctca tgaaatcaaa tgtaacaatg
      121 tcgttgtttc ctttcttttc tttgtgatat gcgtttaaaa aagaaatatt ttcgatctgt
      181 tcatcagtta atgtaaatgg catttcgtca tcaaagaaag cgtaatcggc atttgggaga
      241 ggaatactct caattgagca tagttttaat aaaaaaacat gaaaacaatg aaacatgttt
      301 ttatacataa gagaacttaa tcgctgttgt atcttctgta gttgtccttt gggaaataaa
      361 tgctgtaaat ttgaaaatga gccatctaaa atatatttat atgtaaaaag gtcctctctc
      421 tgaccagtta tcaaaaaatc gtcaggtgat ttagaaaaac gattgagatg acataaatat
      481 tccatttgct ccgtgtctat atacttatgg ggaactacgt agtttggtgg tgtgccaata
      541 agattatcca taaatcacct tgataaaagt taataaaatc tctttatttt aacctcgaca
      601 ataaaagcgg ctataaaaaa tcgctcattt tctgatgctg cggttaatcc gtcgaatgtg
      661 ccgttaaccc actaaaaatt gcaaatccag ggtaaacacc ctgggttaaa atggaaataa
      721 tgagctattt tttacagtat tcaccggtaa tataagtaat acttgttgct aacctaacta
      781 gcagtacagt taattaacaa catagaagtt attgtttttc aaacaactca acaactcaat
      841 attgcacgcc ggaggattta tggtcgctaa attaaaacca gatgttcttt ttaatactaa
      901 tcctttttta gaagcaatga a
//
LOCUS       DARNVM010000345          893 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.347, whole genome shotgun sequence.
ACCESSION   DARNVM010000345 DARNVM010000000
VERSION     DARNVM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.347"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            266..341
                     /locus_tag="SI621_004786"
     tRNA            266..341
                     /locus_tag="SI621_004786"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Ile,seq:cat)
BASE COUNT          272 a          164 c          190 g          267 t
ORIGIN      
        1 cttcaactaa tgtaataaag ttttattatt agatacaaaa tcattacaaa ctttcattga
       61 atcgatggta actataaata gtgctaagta ttagcatttg cttgttgact gagagtatcg
      121 actttgtgaa tggaagcggt gtcgggtttt atgtcgcagg taatgacgac ccaggatgaa
      181 tgagaacgca taaaaatctg ttatttacat cataagatca tcatgtttat tgcgtggttt
      241 ataagtattg ttattgcata ctcaaggccc tttagctcag tggttagagc agacgactca
      301 taatcgtccg gtcgctggtt caagtccagc aagggccacc aaatcatcat tcaggtatca
      361 ggaagagtta agggtacaag gggtactcga tggcggtcta tatttcacga ctaacgccat
      421 ttcaaggaag aagtgtggtg ggtggtttcg aatgagggct ggtgttttgg tacgtggcac
      481 gccactgcaa tgtatttatc catcagtact cattcaaaag tacctggaaa aaatgcccgc
      541 tatgccaagc gggcaaattg cttacatgtg gaattaagta attaacgata aagccagagt
      601 attatcattt atcatgcctc ttcatttaat caggttaatt actcaaatca ctgaactatt
      661 ctctgttcgc tcactgatga taagtgaagt gtcacaacca cgatttgtat gtggaccaat
      721 gctgtagagt aaggatgaaa tgtctctgcc acgccttaaa catctactac tgactagaac
      781 cgccgtcgaa tgcagaagag tcttgtgagc attacaggag tctggctttg gtcattaatc
      841 aacgctattt agatataaat ttctaacact caaaaaacga ttctcacgaa taa
//
LOCUS       DARNVM010000346          881 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.348, whole genome shotgun sequence.
ACCESSION   DARNVM010000346 DARNVM010000000
VERSION     DARNVM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.348"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            163..>881
                     /locus_tag="SI621_004787"
     CDS             163..>881
                     /locus_tag="SI621_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEI4067384.1"
                     /translation="MIRSGFEVQVAAVKALFFREIRTRFGKFRLGYLWAILEPASHLL
                     ILLGIFSYIMHRTMPDISFPIFLINGLIPFFIFSNISRRSVGAIEANQGLFNYRPVKP
                     IDTIIARTLLESFIYVYVYVFLMFIIWLAGEYFQIIRPLQLIGAWSLLIVLSYSIGVI
                     FMVIGKKSPEMQKILPILIKPLYFISCIMFPLHAIPKQYWSYLLWNPLIHVVELSREA
                     VMPSYVSEGVSINYLLIFSLAT"
BASE COUNT          267 a          128 c          176 g          310 t
ORIGIN      
        1 caaataagga tgatacaaac aactttttgg atttctaaga tttaataaag aggggtataa
       61 gaaggcgttt ttctaagtta atggggaaat gaattaacag tccattggta gctgtaaagc
      121 caagggcggt agcgtggaga aattttggga gcataatgtt aaatgataag aagtggattt
      181 gaagtgcagg tggcggctgt taaagcattg ttttttagag aaattcgaac tcggtttggt
      241 aaatttcgct tgggatattt atgggcaatt ttagaacctg catcgcattt gttaatattg
      301 ttagggatat tcagctatat catgcatcgc actatgccgg atatttcatt ccctattttc
      361 cttattaatg ggctgattcc attttttatt tttagtaata tcagtagacg ttctgtaggg
      421 gctatcgaag caaatcaggg gttatttaat tatcgacctg ttaaaccaat agatactata
      481 attgcacgta cattacttga gtcatttatt tacgtctatg tctatgtttt cctaatgttc
      541 atcatctggc ttgctggtga atattttcaa ataataagac ctttacaact gataggggct
      601 tggagtttac tgatcgttct ttcatatagt attggagtaa tatttatggt tatcggaaaa
      661 aaatcccctg aaatgcagaa aatattgcca atactaatta agcctttata ttttatatca
      721 tgtattatgt ttcctctaca tgcaatccca aagcaatatt ggtcatacct tctctggaac
      781 cctttaattc atgtagtaga attaagccga gaggctgtaa tgccgagtta tgtaagtgaa
      841 ggagtaagca taaattactt actaatattt agcttagcta c
//
LOCUS       DARNVM010000347          875 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.349, whole genome shotgun sequence.
ACCESSION   DARNVM010000347 DARNVM010000000
VERSION     DARNVM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.349"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..804)
                     /gene="rhtA"
                     /locus_tag="SI621_004788"
     CDS             complement(<1..804)
                     /gene="rhtA"
                     /locus_tag="SI621_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HEI4067385.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLIGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLA"
BASE COUNT          257 a          257 c          216 g          145 t
ORIGIN      
        1 cgccagcagc tgtatgggtg tcagtgtttc tccgaggaaa atcatcccgg aaacggcagc
       61 cagcgccggt tccatgctca tcagcgtacc aaatgtccgt gttggcaaac gggtgagggc
      121 aatcatttcc agcgaataag gcagagcggt cgagagaata gcgaccgcca gacccaatgg
      181 aataaccgac cagtgccaga gtgcttcacc agcctgaagc gctccaattg gcacgaaaat
      241 taacgctgca atcaacgaac caatagccac cgtcgcaggg ccatgttccg ctcctgcgcg
      301 ttgcccactt aaaatgtaaa tagcccaaca agccccggcc cccagtgcca gcgcacagcc
      361 gattaaatcg acatgggaaa cgtcttgccc cagcggtagc aggaaccaca gaccaagaac
      421 cgccagcaca acccagacga aatctaccgg gcgacgagaa gagaacagcg ccaccgccag
      481 tggtccggtg aactccagcg ccaccgcaat acccagcggt accgtctgaa tagaaagata
      541 aaaaagataa ttcatccccc ccagcgaaac gccgtaaaac aacagcggta accgttgctc
      601 tttggcaaag cgcagtcgcc atggcttaaa gaacgcgatg aggatcagcg ttcctaatgc
      661 cagacgcagc gcagtgacac ccggtgcgcc caccagagga aaaagtgact tagctaacga
      721 ggctccaccc tgaatagacg ccatggcaac gagcaatatg actattggta accagaccgg
      781 cattttacgt aatgaaccag gcatcctttc tcccacaaat atctagactt aagtaaagcg
      841 tggagtgtac tggatatacc caatgctggt tgagc
//
LOCUS       DARNVM010000348          864 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.350, whole genome shotgun sequence.
ACCESSION   DARNVM010000348 DARNVM010000000
VERSION     DARNVM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.350"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>864
                     /locus_tag="SI621_004789"
     CDS             <1..>864
                     /locus_tag="SI621_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HEI4067386.1"
                     /translation="ATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSAMRHRDIII
                     AKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDREFIASVVD
                     RAIFHFAINSICNPEDNKDATPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHL
                     QAIGSGFLTFVNACQEYDDNSLKLFASLLISLSYSSAYVDLAGAERVKINEYNENYLT
                     AQFEELSQRDMKKYLGEMKRLADGGEMNFDDYLDKMSLLVNEGKLEPDILSKMRDAAP
                     KLIDFAKSFDP"
BASE COUNT          298 a          124 c          176 g          266 t
ORIGIN      
        1 tgcaactgga tactttaata aaacactcaa ttcaaatcaa ttcgataatg aagatgcaat
       61 caaaggtaaa gtaaatagta tatatggtaa atctattgat tattcagcga tgcgtcatcg
      121 ggatataatt attgcaaaaa tagatttgtt tattcaaaga atcactcata atttatggac
      181 tgcacgtgaa aaaaatgtga cgttaattaa gcaaataaat gatctcaaaa tgtgtgtcaa
      241 tgagtatatt ggagattgta ctgatgagga attaaacgat cgtgaattta ttgcatcagt
      301 tgttgaccgg gctatttttc atttcgcgat taatagtata tgtaatcccg aggataataa
      361 agatgcgacg cccatagaac gatgtacttt tgacgtagaa actaagaatg gccttccctc
      421 cacggttcag cttttttatg aggaatctaa agataatgaa cctttagcga atatacattt
      481 gcaagcaata ggttctggtt ttttaacgtt tgttaatgca tgtcaggaat atgatgacaa
      541 cagcttaaaa ttattcgctt cgctgttaat ttcactttca tattctagtg cctacgtaga
      601 tttagcagga gcagaaagag tgaagattaa tgaatataat gagaactatc tgacagctca
      661 gtttgaagaa ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc
      721 agatggggga gaaatgaatt ttgatgacta tctggataaa atgtcacttc tggtgaatga
      781 aggaaagctc gaacctgata ttttaagcaa aatgcgagat gctgcaccga aattaattga
      841 ctttgctaag tcgtttgacc caaa
//
LOCUS       DARNVM010000349          853 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.351, whole genome shotgun sequence.
ACCESSION   DARNVM010000349 DARNVM010000000
VERSION     DARNVM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.351"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..285)
                     /locus_tag="SI621_004790"
     CDS             complement(<1..285)
                     /locus_tag="SI621_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEI4067387.1"
                     /translation="MAKNDFKAFATDRNANVISQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKN"
     gene            complement(285..>853)
                     /locus_tag="SI621_004791"
     CDS             complement(285..>853)
                     /locus_tag="SI621_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HEI4067388.1"
                     /translation="LITAFDIDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGL
                     GYDQGVWQGPYDPDSGYMYLSDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGL
                     RMQIVDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETP
                     SEGSKFFGFDMDNEFISGFDVGAWGVLL"
BASE COUNT          235 a          244 c          164 g          210 t
ORIGIN      
        1 attcttgcgg ctcaggtatt caacagcaaa ccccgatccc agcaattcaa caaaaccggg
       61 cagatcacca ttatcaagca catcccgttg cgttttatca cttacaaact gggccagagc
      121 tgcagcaata aagctggcct gccgaataac cttattgact tgcgcactgg aggctttccc
      181 tgctgtaaat ccggataaaa gcgcgggcaa cgcttcccat tcctcctgcg atataacatt
      241 ggcatttcga tccgttgcaa acgctttaaa gtcatttttc gccatcagag taatactccc
      301 catgctccta catcaaaacc actgatgaat tcgttatcca tatcaaaacc aaaaaatttt
      361 gagccttccg atggggtttc caccgaaggt gtttcaatgc cacccgccca taccccggcg
      421 gcttttactg tgagataccc ctgtttaatt gccgcaatta actcacgcga tacatctgaa
      481 atatcagtat caggaaagac ccagaccgat atcgtcatgt cctggttatc gactatctgc
      541 attcgcagcc cggatcctgc tgttgcagcg tcaagaattg ccggaagcga atcattccgt
      601 ccatcccagt tattaatcgc aatcttcgct ttaagaatga cacgataagt ttcatcgctg
      661 aggtacatgt atccggaatc aggatcgtat ggtccctgcc ataccccctg atcatatcca
      721 agcccgtcgg tatcccagct gaaatagaca cctgagatag gctggctgac aacacggcta
      781 cgtccgatcc acaatcccag aatgtcaagt tgcacaccaa ccgcagagtc aatatcaaat
      841 gcagtaatca gcc
//
LOCUS       DARNVM010000350          806 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.352, whole genome shotgun sequence.
ACCESSION   DARNVM010000350 DARNVM010000000
VERSION     DARNVM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.352"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..181
                     /locus_tag="SI621_004792"
     CDS             <1..181
                     /locus_tag="SI621_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067389.1"
                     /translation="KPYSITDDNNCWKIEGKQPKTLGGNFTILIAKKDGQALDVIHKI
                     IHCSDDSNILPRCVS"
BASE COUNT          274 a          140 c          170 g          222 t
ORIGIN      
        1 gaaaccatat agtattacag atgacaataa ttgttggaaa atcgagggaa aacaaccgaa
       61 gacgttagga ggaaatttta caatactaat agcgaaaaaa gatgggcaag cgttggatgt
      121 aatacacaaa ataatccact gttcagacga ttcaaatatt ttaccaaggt gtgtttcata
      181 actattttgt gtacaatatg actctacata tcgacgaaaa aaccacttga tttgacagga
      241 acacattcaa aaaaatttct ttttcatttc aggagcgatt ttcatctgtt acttgaagta
      301 tctgtgagat taagtgactc cccctaacgt cgtactaaat agtaattact ggccataccg
      361 ggagcagtat tcggcgcttc ccacatgcag cagaataacc gcatcagcga agatgtattg
      421 tatttttact atcacaatgt gtacggacgg tttatggaaa agaatgaaca ttagatacgc
      481 gatgcggcgg cagattacac ggttatcact acgatagcca atactggctg gtgcattacc
      541 gccgtgaaca ataggacgcc gtactgctgg aaatttctta cctttatgac tccccgcacc
      601 gccgtatcgg gaaacgggaa gcagtcaaga tgaggctgat ataatgtgtg gaatgcctga
      661 gtatttttag aatagagggt aatatgtttg atagaatata tcttggtgat cgaccaataa
      721 aaaaattgaa tttgatttat ggagcaaaca aattagaatt caggtgaatt taatctcaag
      781 gatagcctat ggtacaacag aatgga
//
LOCUS       DARNVM010000351          793 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.353, whole genome shotgun sequence.
ACCESSION   DARNVM010000351 DARNVM010000000
VERSION     DARNVM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.353"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            61..615
                     /gene="pinE"
                     /locus_tag="SI621_004793"
     CDS             61..615
                     /gene="pinE"
                     /locus_tag="SI621_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HEI4067390.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGVSTLYKKFPVGDK"
BASE COUNT          212 a          157 c          217 g          207 t
ORIGIN      
        1 tgaaaatgca gaaataacga gcaaatggca tcattcctgc ttttgtcagg gagatctacc
       61 atgcttattg gctatgtacg tgtgtcaaca aatgaccaga ataccgatct acaacgtaat
      121 gcgttgaact gtgcaggatg cgagctgatt tttgaagaca agataagcgg tacaaagtcc
      181 gaaaggccgg gactgaaaaa actgctcagg acattatcgg caggtgacac tctggttgtc
      241 tggaagctgg atcggctggg gcgtagtatg cggcatctgg tcattctggt tgaggagttg
      301 cgcgaacgtg gcgttaattt tcgcagcctg acggatgcta ttgataccag cacaccgatg
      361 gggcgttttt tctttcatgt gatgggggcc ctggctgaaa tggaacgaga actgattgtt
      421 gaacgaacaa aagctggact ggaagctgct cgcgcacagg gacgaattgg tggacgtcgt
      481 cccaaactta caccagaaca atgggcgcag gccgggcgac taattgcatc aggagttcct
      541 cgccagaagg tggcgatcat ttatgatgtt ggcgtatcga ctttgtataa gaagtttccg
      601 gtcggagata aatgaaaccg tagcacgtcg tatgcaagaa cgtgccacgg ctggctgata
      661 agcttgtaaa aatattgaac agggagtgga gcattctatg aagctaattt gtttaatttc
      721 atttgtagtg actttacttc ttggtcgata atgtcgaatc ccttattgac agcatctctt
      781 agctctgcat caa
//
LOCUS       DARNVM010000352          784 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.354, whole genome shotgun sequence.
ACCESSION   DARNVM010000352 DARNVM010000000
VERSION     DARNVM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.354"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..530)
                     /locus_tag="SI621_004794"
     CDS             complement(<1..530)
                     /locus_tag="SI621_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067391.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPTYFNKTSIP
                     SDCWQILFFSPDHFNN"
BASE COUNT          224 a          148 c          160 g          252 t
ORIGIN      
        1 tcattattaa aatgatcggg tgaaaaaaag agaatttgcc aacaatctga aggaatggag
       61 gttttattaa agtacgtagg gggggttaag atggcttctt ttcgggtgaa ttttgcacaa
      121 tacttagaaa tcgtctcctt aatacactcc agctcttttt tattgacggc tgagcaagta
      181 cattgttcgc caaaaggtgc aaaagtgcct acatctaatg tttgtatgat ataatcgtca
      241 tgttctcctg tagaaatatc tatgatgaat ggactcatat tttccggttg gattcggatc
      301 atgacagaag gaacatcgcc ttgatagaaa ttcaatgcag taattgcaga taatactctc
      361 cgcagttcat tacttatact gtatatttta ttaacgtcat tggtggagac aatttctgca
      421 aactttcttt ttaccgtcca ataatcaata tttctgttgt gattgttaac cactgcgtgc
      481 attggcggga ttgaagggtg aaaaatatgt gcaattggga agcctttcat tgatgcctgc
      541 cttgtcatca tgacaaacaa taatagatgc ttattttcct cgcagggttc tttcgcacta
      601 tcaaaaaacg ctcatttacc cggcgttaca gtaaacgaag caacattctg tggcctacgc
      661 cgatcaaaag ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat cgcggcgacc
      721 agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgtcgccta tgaggggaga
      781 ggaa
//
LOCUS       DARNVM010000353          777 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.355, whole genome shotgun sequence.
ACCESSION   DARNVM010000353 DARNVM010000000
VERSION     DARNVM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.355"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>777
                     /locus_tag="SI621_004795"
     CDS             <1..>777
                     /locus_tag="SI621_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HEI4067392.1"
                     /translation="NETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYL
                     SESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGR
                     YFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKD
                     FDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQS
                     PWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYK"
BASE COUNT          254 a          146 c          145 g          232 t
ORIGIN      
        1 ttaatgaaac agcaaaacaa tttaatacct caattattgt atatttaatt gaccccaaat
       61 actttgctga tctgccgacg tcacagtttt ggtcgtacgc gacatacttc agggtattgt
      121 cttttgaata tctgagtgaa agtatttcca cactgctgta tctggatgcc gatgttgttt
      181 gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa agatgagttt gctgcggtca
      241 ttcctgacaa tgatagtact caggcggcat gtgcaaaacg cctcaacatt cccgaaatga
      301 atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg catgaagcaa
      361 atttgacacc gtatttactc aaacttttac gaggggaaac taaatatggc tctcttaaat
      421 atttagatca ggatgcgttg aatatcgcat ttaatatgaa taatatctac cttgcgaagg
      481 attttgatac tatttatacc ctgaaaaacg aactttatga tcgtagtcat cgaaagtatc
      541 agcaaaccat taccgataaa acagtgttga ttcactatac agggataact aaaccatggc
      601 atagctgggc tggatatccg tctgcatcat actttaatat cgcgcgtgaa caatctccct
      661 ggaagaaata tcctcttaaa gaggcgcgga ctgttgcaga aatgcagaaa caatataagc
      721 atctgtttgc ccatggtgag tatataaaag gcataacttc attaattaag tacaagc
//
LOCUS       DARNVM010000354          765 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.356, whole genome shotgun sequence.
ACCESSION   DARNVM010000354 DARNVM010000000
VERSION     DARNVM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.356"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..233)
                     /locus_tag="SI621_004796"
     CDS             complement(<1..233)
                     /locus_tag="SI621_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEI4067393.1"
                     /translation="MKACLLLFFYFSFIFQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQ"
     gene            complement(245..>765)
                     /locus_tag="SI621_004797"
     CDS             complement(245..>765)
                     /locus_tag="SI621_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HEI4067394.1"
                     /translation="PVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPT
                     SIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVI
                     GPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISN
                     MQGTPEKPSDIR"
BASE COUNT          222 a          152 c          125 g          266 t
ORIGIN      
        1 aattgtttgt aaatgttatt tttagtaaag cagtaatatt gatatagtcg aaatgatgcc
       61 tctgaatcac catgttgtga tttatacttc agtttcatca acttttcttc acttaaatca
      121 taggtcattg ctgtagaccc catcatactt tcgttttgtt ttattttcac atcagcacca
      181 tgcaattgaa aaataaaaga gaaataaaaa aatagtaaca agcatgcttt catacatcta
      241 caacctatct tatatcactg ggtttttctg gcgtaccttg catattagaa atggggtgcc
      301 cagatggccc atatgttatc caattaccaa ggtcttttgt tgttatagcc attagatcct
      361 tatttgaatt aaggttcgtt ggtgctgtta gcactgtatc catttttaac ttaccattag
      421 ttcctcgccc ccaccacgtc catagcgtgt caggtcctat tacatttgta tgtaactctt
      481 tagctaattg gctagcaaat gaattttgtc ctttacctgt attacaagaa aataaccaaa
      541 ctgtcatccc atctttataa ttcccatcaa atttaattag atatgctaga tctttagctg
      601 tcatgatact gcgcgttgcg gattcaatag atgtgggggt accatgcccc ccgattgtga
      661 aaacgcctgg gatattaatc tcatcagcta cagaatggat aagatcactt tcggggtata
      721 aatttatatc aaccaaacct tgcggatcta catttatgac cggat
//
LOCUS       DARNVM010000355          761 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.357, whole genome shotgun sequence.
ACCESSION   DARNVM010000355 DARNVM010000000
VERSION     DARNVM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.357"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..106)
                     /gene="ariR"
                     /locus_tag="SI621_004798"
     CDS             complement(<1..106)
                     /gene="ariR"
                     /locus_tag="SI621_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HEI4067395.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLG"
     gene            complement(135..407)
                     /locus_tag="SI621_004799"
     CDS             complement(135..407)
                     /locus_tag="SI621_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HEI4067396.1"
                     /translation="MKTSDNESTKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGEPNRRTAIADNKSMFNGKKINRIH"
     gene            complement(450..686)
                     /locus_tag="SI621_004800"
     CDS             complement(450..686)
                     /locus_tag="SI621_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HEI4067397.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          215 a          159 c          124 g          263 t
ORIGIN      
        1 gccctaacac agctgactct tcttctaaca aattacctga actacgaaag tagcttgata
       61 acgctttatc agttattgca ttatgaattg tagtatcttc aagcatggta acctctcatc
      121 ttacttatga aattttaatg tattctgttt attttcttac cattgaacat acttttattg
      181 tcagctatcg ctgttcttct gtttggctct cctgtatcac tgttgctaaa atcacgaatt
      241 atgctgtcaa ttaaatctcg tcggaaagca ttttcctcag ccgatttcat tacgagtaat
      301 tgcttaataa gagtctgtaa cgaaaaattt atcttcatgc gcagtatatg gagcaccgcc
      361 tggccagtaa tttcatattt tgtactttca ttatcagatg tcttcataac gacttcatag
      421 ctaacctgtt aaataatcag gctgttgcat tattcaaaaa gcaacccaat tagtgcatta
      481 taatgtttct gttcctcttc ccctgtcgct tgttccagtc ggcatagaag tttggcacaa
      541 agcgactttc gactcagatt acgtccatct ttcaaaatct cagtcacaat ttccccgaga
      601 gtttcctgct gagtaggcaa gttagctttt gcaaagtaac gagtgattgc tcccgcagaa
      661 tctaaagtca ctgaattttg atgcatgcta cgcctctgtt aaaaatgtta atcaatgtta
      721 agtgaggtaa caatagcaaa cttgtacgaa atatgtgtac a
//
LOCUS       DARNVM010000356          720 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.358, whole genome shotgun sequence.
ACCESSION   DARNVM010000356 DARNVM010000000
VERSION     DARNVM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.358"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>720)
                     /locus_tag="SI621_004801"
     CDS             complement(<1..>720)
                     /locus_tag="SI621_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067398.1"
                     /translation="DISSDLARRKINDLPESPIVNNLEALAPGIEKLKQTSIEMVTLL
                     NALQPGGKCIITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSDNTCELTINE
                     PLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGGIVDLSSLDLRNVDLELYDFT
                     DKHMANTILNPFKLDDTDFTNANMFQVNFVSSTQNATISWDYLLKITPVITSISDMYS
                     EEKIKLVESCLNALGDITEEQ"
BASE COUNT          220 a          122 c          125 g          253 t
ORIGIN      
        1 attgttcttc ggtaatatct ccaagcgcat ttaaacaact ttcgacaagc ttaatttttt
       61 cttcagaata catatcgcta atgcttgtta taacaggcgt tatttttagt aaataatccc
      121 aggagattgt ggcgttttgt gttgaactaa cgaaatttac ctgaaacatg ttggcattag
      181 taaaatctgt gtcatccaat ttaaaaggat ttaaaattgt attggccata tgtttatctg
      241 taaaatcata aagttctaaa tcgacattcc gtaagtccag agaggataga tcaactatac
      301 ctccagtccc acaactaaag attacgttca acctcctgat ttcattgtaa atgtctttca
      361 atgggcaatt aacattgata ttgcctgtgg atatttcctg gttttttaac ggttcattaa
      421 tggtaagttc acaggtattg tctgatcgtt gaataattcg ggcgttataa ccaaaaaaat
      481 ccatacgaag agacgaatca ttataaagaa ttacattttg taagtacgct aattcttttt
      541 gaaaatcacc agtgataatg catttcccac ctggttgtag cgcgttaagt aaagtgacca
      601 tttcaataga ggtctgtttt agtttttcta tacctggtgc tagtgcttcg aggttattaa
      661 ctatggggct ttcaggaaga tcatttattt ttcgacgggc taaatcacta ctaatatcat
//
LOCUS       DARNVM010000357          718 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.359, whole genome shotgun sequence.
ACCESSION   DARNVM010000357 DARNVM010000000
VERSION     DARNVM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.359"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            1..661
                     /gene="espY4"
                     /locus_tag="SI621_004802"
                     /pseudo
     CDS             1..661
                     /gene="espY4"
                     /locus_tag="SI621_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY4"
BASE COUNT          250 a           89 c          144 g          235 t
ORIGIN      
        1 tttcgaggat tgtaaattca acaacataca gctttatgag actaatgatg atactgtggg
       61 gtcaggaaat aaaaaaccaa taaaaggtat gtttaaaggt tgttttattt ctaaatgcaa
      121 aattgaaaat tatagatgtg agacgtctaa aatctacacc gttacacaac ctgatagtat
      181 tcaagaaaaa ttaggttctt atctctttat gcagtctttc gttcaggatt gtataatcca
      241 gggaggatgt tgtcctggct cgagtatact tctgagtcat ttttataact gtaatataat
      301 agggcttgat gctcatggaa tggatttttt ggcaaattcg ttcaacaagt ctaattatga
      361 tgaaatcagg ggtccggata cggggactgt gttttataat tgtaatttga agcatattaa
      421 aataaacgat ggtcttcgtg aagatggttc agatagatct atgtttaacc ccaaagagta
      481 tttttctgat tacattgaaa gaagatcgag aaatcttaag ttagatgata atttttcgaa
      541 atgcgtagag agtataaaga aagggaccga ttcttcattt agcaagaaaa atacagagaa
      601 caataatgtc ttctttaaaa atagtaattt gattggggca aatttaggct attattacag
      661 ttgagatagg tgcaaagact gtgctattga tccaaataca aattattcaa aagatggt
//
LOCUS       DARNVM010000358          699 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.360, whole genome shotgun sequence.
ACCESSION   DARNVM010000358 DARNVM010000000
VERSION     DARNVM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.360"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>699)
                     /locus_tag="SI621_004803"
     CDS             complement(<1..>699)
                     /locus_tag="SI621_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4067399.1"
                     /translation="SNGKLERQKFLFPLDYENKLQHQLDKYFTIESHPLLYRHTMGSK
                     IANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIAKDAVTNNIALMST
                     FYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAF
                     QDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDA
                     TNLVNLKTLLNDGC"
BASE COUNT          226 a          136 c          104 g          233 t
ORIGIN      
        1 aagcaaccat cattgagtag tgttttaaga ttgactaaat tagttgcatc ttcacgtacc
       61 atcgtggcaa ctggaacagg caacgacatg aaaacttctt cattcaccat caagtggatc
      121 atgcattgcg ttccctttgt catatccaca cttacgctac aaatatcgtt gtaatgcgaa
      181 gagtgcattt caggaaacaa attaaacacg gtatcttgaa aggcactaaa tcgtgttaat
      241 aaatcctgat aatgaacagg ttttgtttca aaattatcat cagcaaatcc aagcactatt
      301 gcatgctcta catctaacgc agtttcaaat gactcttctt ctgtaaaatc attatttaaa
      361 acctcacctg acgtattatc actgtcataa aaagtgctca tcaatgcgat attgtttgtt
      421 acagcgtctt ttgcgatatt ctcactaatt gcatatcgcc tgctataatc ataaaaatga
      481 ataaacgcat cctttataaa ggatatatat tgctctttgt taaagtcaat acgactgtat
      541 aatttttcaa aaatcacatt cgcaatctta ctacccatag tatgcctgta taataatggg
      601 tgcgattcaa tcgtaaaata cttgtctaat tgatgttgta atttattctc atagtccagt
      661 gggaaaagaa atttttgtct ttcaagtttc ccattcgat
//
LOCUS       DARNVM010000359          678 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.361, whole genome shotgun sequence.
ACCESSION   DARNVM010000359 DARNVM010000000
VERSION     DARNVM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.361"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>678)
                     /locus_tag="SI621_004804"
     CDS             complement(<1..>678)
                     /locus_tag="SI621_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEI4067400.1"
                     /translation="AHVQKQGAVRVTELSRLFKASPETIRRDLTVLERNKKLIRSFGG
                     AVALDSSDSAPVMLADNAWQDNSIDRAESFRKRTEENPDIKARIAKAALQFIHPGDCI
                     LMDNSSTCWFLARQIPDIDVTVVTNSLRIIQALACRDRVRIIGIGGEYSERHDDFHGP
                     VAEGAIRNFQINSFFFSCQGLNLGNGVRDGSEINARLKQVMLQVSGQKILLVDSSKFE
                     QYAFSKIC"
BASE COUNT          199 a          170 c          121 g          188 t
ORIGIN      
        1 gcatatttta ctaaatgcat actgttcaaa cttactggag tcgaccagca atattttttg
       61 tcctgacacc tgaagcatca cctgtttaag tctggcatta atttcactgc catcgcggac
      121 accattcccc agattcaacc cctggcatga aaagaaaaaa ctgttgattt gaaaatttcg
      181 gatcgccccc tctgcaaccg ggccatgaaa atcatcatgt cgctcagaat actcaccacc
      241 gataccaata attcgaactc gatcacggca agccaacgcc tgaattatcc gtaatgaatt
      301 tgtaactaca gtaacatcta tatcgggaat ttgacgagct aaaaaccaac aagtactact
      361 gttatccatt aaaatacaat caccagggtg aataaattgc aatgcggcct tcgcaattct
      421 ggcttttata tcaggatttt cttcagtccg tttacggaat gattccgcac gatcgatact
      481 attatcttgc cacgcattat cggcgagcat aaccggagcg ctatcagatg aatccagagc
      541 gaccgcgcca ccaaagctac ggattaactt tttgttgcgc tccagtaccg ttaaatcccg
      601 acgaattgtc tcaggagacg ctttaaataa tcgtgataat tctgttaccc gtacagcacc
      661 ttgtttttgt acatgtgc
//
LOCUS       DARNVM010000360          674 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.362, whole genome shotgun sequence.
ACCESSION   DARNVM010000360 DARNVM010000000
VERSION     DARNVM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.362"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..454
                     /locus_tag="SI621_004805"
     CDS             <1..454
                     /locus_tag="SI621_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HEI4067401.1"
                     /translation="YPLNPISEIDPQGLNPLILSVVALMSCGLFSSGGVIQSGQQRRD
                     QYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNRLCNQSRACAPQMLRKEI
                     LRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAADCSGLIK"
     gene            451..>674
                     /locus_tag="SI621_004806"
     CDS             451..>674
                     /locus_tag="SI621_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067402.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDITGLEELDIVYNF
                     FQKKQWNEIANNFKIKDDSYALELGITFLP"
BASE COUNT          234 a          112 c          145 g          183 t
ORIGIN      
        1 gtatccgtta aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttag
       61 tgttgtagca ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca
      121 acaacgcaga gatcaatatg gccgtaaagt taatccagaa gccgacaaat taacagatga
      181 acataagtca ggtgtaaatc ctggtggtaa ctgtagtccc cagaacctga gtgacttaca
      241 aaatgaaaaa aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa
      301 agaaatattg agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa
      361 taatcagtgc tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa
      421 aacagcagct gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt
      481 gcagtctatg atgaacttat gattcatttc ccatgccagc cattacctca tatttcaaat
      541 gatattacag ggctggagga actggatatt gtatataatt ttttccaaaa gaaacaatgg
      601 aatgaaatag ccaacaattt taaaataaag gatgactctt atgctttaga gttaggaatt
      661 acattcctcc ctga
//
LOCUS       DARNVM010000361          671 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.363, whole genome shotgun sequence.
ACCESSION   DARNVM010000361 DARNVM010000000
VERSION     DARNVM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.363"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            197..>671
                     /locus_tag="SI621_004807"
     CDS             197..>671
                     /locus_tag="SI621_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEI4067403.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLV"
BASE COUNT          203 a          147 c          138 g          183 t
ORIGIN      
        1 acaacataaa acactttacc accaccaaaa aaattctcta ttgcattgtt ttttaatgaa
       61 aaaacattat tctcgtaata tttcacgata aataacatta ggattttgtt atttaaaccc
      121 gagtcctttg cacttgctta ctttatcgat aaatcctact tttttaatgc gatccaatca
      181 ttttaaggag tttaaaatgg ataagaagca agtaacggat ttaaggtcgg aactactcga
      241 ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa tcaaaacgat ttccgctgca
      301 cgaaatgcgc gatgatgtcg cattccagat tatcaatgac gaattatatc ttgatggcaa
      361 cgctcgtcag aacctggcca ctttctgcca gacctgggac gacgaaaatg tccataagtt
      421 gatggattta tccattaaca aaaactggat cgacaaagaa gaatatccgc aatctgcagc
      481 catcgacctg cgttgcgtaa acatggttgc cgatctgtgg catgcgcctg cgccgaaaaa
      541 tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc tcggcgggat
      601 ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc
      661 aaacctggtg t
//
LOCUS       DARNVM010000362          659 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.364, whole genome shotgun sequence.
ACCESSION   DARNVM010000362 DARNVM010000000
VERSION     DARNVM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.364"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>659
                     /locus_tag="SI621_004808"
     rRNA            <1..>659
                     /locus_tag="SI621_004808"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          147 c          220 g          138 t
ORIGIN      
        1 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
       61 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      121 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      181 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      241 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      301 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      361 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      421 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      481 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga
      541 gatgagttct ccctgactcc ctgagagtcc tgaaggaacg ttgaagacga cgacgttgat
      601 aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgagg
//
LOCUS       DARNVM010000363          657 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.365, whole genome shotgun sequence.
ACCESSION   DARNVM010000363 DARNVM010000000
VERSION     DARNVM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.365"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..475)
                     /locus_tag="SI621_004809"
     CDS             complement(<1..475)
                     /locus_tag="SI621_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEI4067404.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLV"
BASE COUNT          189 a          149 c          139 g          180 t
ORIGIN      
        1 acaccaggtt tggtttgttg gtcggcttac ctgcggcttc catacgcttg cgccaacgcc
       61 atttcatcgc catcccgccg agcatacagg cctcggaaga accaatggtg ttggtgccaa
      121 cggcctgacc atttttcggc gcaggcgcat gccacagatc ggcaaccatg tttacgcaac
      181 gcaggtcgat ggctgcagat tgcggatatt cttctttgtc gatccagttt ttattgatcg
      241 acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcaaaaa gtggccaggt
      301 tctgacgagc gttgccatca agatataatt catcattgat aatctggaat gcgacatcat
      361 cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg gcctttgcgc
      421 caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg tccatttcga
      481 actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat ggaagcaagg
      541 cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt ttataatata
      601 aacatttaac atgataatat taaaaacaga ctttattatc gcaataaaaa tcacccg
//
LOCUS       DARNVM010000364          651 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.366, whole genome shotgun sequence.
ACCESSION   DARNVM010000364 DARNVM010000000
VERSION     DARNVM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.366"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..171
                     /locus_tag="SI621_004810"
     CDS             <1..171
                     /locus_tag="SI621_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067405.1"
                     /translation="KIPNISKEILHLKDETERLEKEYQQENTLNDVVQNIRSWLKENE
                     NMVKAIKKTDTE"
BASE COUNT          229 a          108 c          133 g          181 t
ORIGIN      
        1 aaaataccaa atataagtaa agagattcta catcttaagg atgaaacaga gagattggag
       61 aaagagtatc aacaagaaaa tacacttaat gatgtagttc aaaatatcag gagttggcta
      121 aaagaaaatg aaaatatggt gaaagctatt aagaaaactg atactgaata acatggcata
      181 taaataaacg gatcaagtcg ttcctgtgat aatgaggtca tgcgaaaaga cgcttactca
      241 tcagtaatct ggtgtacttt aattaaacca gtgtagttga tgtgtaagcg ttgatgttgg
      301 gagacgatta aatggttata tcactactac gccctttcca gacggcgtat aagagtgcaa
      361 catgttgagt tttccgagtg aatggtgcat tttactaacc gagccatttg aatcaaccaa
      421 cgcaataaaa aagactggaa taataagcct gtatttatta ttcatcgcaa tcgcactcta
      481 cggttttatc ctgcagaaat gagactactt ccagcggttg tggattatcc ctgggcgata
      541 gtcatagtct tgtgtcagga ttcacaaatg acctgttacc tgacatggat ataaacacaa
      601 catagagtgc ggtagttaat ctgaatcatc tatttttcgg ctacacagca c
//
LOCUS       DARNVM010000365          650 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.367, whole genome shotgun sequence.
ACCESSION   DARNVM010000365 DARNVM010000000
VERSION     DARNVM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.367"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..620)
                     /locus_tag="SI621_004811"
     CDS             complement(<1..620)
                     /locus_tag="SI621_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HEI4067406.1"
                     /translation="MERLQRIRQNPEILPVLKQFYRTNPAQFIIDWGMTTDPRNIDYG
                     LPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSREMGLSWTAIGLACSLCLFNKEM
                     VIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFRGSWSEKKHAPYMRVEFPETGA
                     VIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAALSQTTRCRIDL"
BASE COUNT          164 a          182 c          155 g          149 t
ORIGIN      
        1 aggtcgatac ggcaacgcgt cgtttgtgac aacgccgcat caatcagcag aggacgctga
       61 aggaatgcag cctcatcaac cagataaagc gtggtacggt caccacgacc aatattatcg
      121 ccagcctcgc ctttgataac ggcaccagtt tcaggaaact caacacgcat atatggcgcg
      181 tgcttcttct cgctccacga accgcgaaac tctacaggca gtgtttccac gaacttgcgc
      241 gccttccaga acaatgcttt cgggtcaccg gtgctgtcga cgtattcctc tttacgggag
      301 ccgaaaccga taaccatttc tttgttgaag agacaaagcg agcaggccag tccgatcgcg
      361 gtccaactga gccccatttc acgggatttt tcggtaatac cattctcccg attgccccag
      421 cgttccataa tccagtggat ccactcctcc tgcttaggga agagtaaaaa cggaatggtc
      481 accggcaggc cataatcaat attacgcggg tccgttgtca tgccccagtc gatgatgaac
      541 tgagccggat tggttcggta aaactgcttc aatacgggca atatttcagg attctggcga
      601 atgcgctgta ggcgttccat ccgccattca aaaaccatct ggtaatcagg
//
LOCUS       DARNVM010000366          635 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.368, whole genome shotgun sequence.
ACCESSION   DARNVM010000366 DARNVM010000000
VERSION     DARNVM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.368"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            117..>635
                     /gene="acrS"
                     /locus_tag="SI621_004812"
     CDS             117..>635
                     /gene="acrS"
                     /locus_tag="SI621_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HEI4067407.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLWELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQNLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGI"
BASE COUNT          188 a          132 c          144 g          171 t
ORIGIN      
        1 gacgatttat agcaactcaa tattagcctc tcgtataaat atacattagg tgatagatta
       61 accttcgcta ttttctcatc ctgtgtcgaa tatatttatt tcctgaataa ttaatcatgg
      121 caaaaagaac caaagctgaa gccctgaaga cccggcaaga actgattgaa actgccatcg
      181 cccagtttgc gcagcatggc gtaagcaaga cgacgctcaa cgacattgcc gacgccgcta
      241 acgttacgcg tggcgctatc tactggcact tcgaaaacaa gactcaactg tttaatgaga
      301 tgtggttgca acagccttca ttgtgggagt taatccagga acacttgacg gctggattag
      361 agcatgaccc gtttcaacaa ttgcgtgaaa aattgattgt cggcttgcaa tatattgcca
      421 aaattccccg ccagcaggcg ttgctgaaaa tcttatatca caaatgtgaa tttaatgatg
      481 agatgctggc cgagggagtg atacgcgaaa agatgggctt taatccgcag aatctccgcg
      541 aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa taacctcgat ttagatgttg
      601 tgatgattat tattgatggt gccttcagcg gaatt
//
LOCUS       DARNVM010000367          624 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.369, whole genome shotgun sequence.
ACCESSION   DARNVM010000367 DARNVM010000000
VERSION     DARNVM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.369"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>624
                     /gene="ydeO"
                     /locus_tag="SI621_004813"
     CDS             <1..>624
                     /gene="ydeO"
                     /locus_tag="SI621_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HEI4067408.1"
                     /translation="HFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLN
                     EMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLA
                     HPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQ
                     CGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNKGNTMNALAI"
BASE COUNT          201 a          118 c          124 g          181 t
ORIGIN      
        1 gcatttcaaa gaaatcggat ttaatgaaaa taccattaaa aaatatctac aatgcactaa
       61 catccagaca gtgacggtgc ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac
      121 tggattgctt aatgaaatga ttgcttatct caactcggaa gaacgcaatc atcataattt
      181 ttcagaactt ttgctttttt cttgcctgtc tatttttgcc acatgcaaag gtttcattac
      241 actattaact aacggtgtgc tatccgtttc cgggaaagtg agaaatattg tcaacatgaa
      301 gctggcgcac ccatggaagc tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct
      361 gttgaagaaa aaactaaagc aagagcaaac gacattctca cagattcttt tagatgccag
      421 aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca
      481 atgtggttat gcgagtacat cttattttat ttatgcgttc cgcaaacatt ttggcaacag
      541 tccaaagaga gtttctaagg agtaccgttg tcaaaggcac acgggtatga ataagggcaa
      601 cacgatgaat gctttagcta tttg
//
LOCUS       DARNVM010000368          591 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.370, whole genome shotgun sequence.
ACCESSION   DARNVM010000368 DARNVM010000000
VERSION     DARNVM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.370"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            62..382
                     /locus_tag="SI621_004814"
                     /pseudo
     CDS             62..382
                     /locus_tag="SI621_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
BASE COUNT          166 a          134 c          142 g          149 t
ORIGIN      
        1 tgaaaaataa gatgacgttg gcatatagat cactgcgaat aatccacctc taccactgcg
       61 aataatccac ctctaccact gcgactatcg cggcctgccg ctgacgctta tcagccctga
      121 cggggcaaca gaatggtgcg cagaatatga tgaatggggc aacctgctga ctgaagagaa
      181 cccgcagcac ctgcagcagc ttatccgtct gccggggcag cagtatgatg aggagtccgg
      241 cctgtattac aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga
      301 accgattggg ctggaaggcg ggtggaatca gtatgtatat gcttccatac atccaaccta
      361 ttctattgat ccgctgggct taattgataa acctgctcca gtattcaatc gagaactaaa
      421 ttcagatgcg tattatcttg ccgtaaataa ttgctattca tatgctctaa ataggtatgg
      481 aaatcctggg agtaggattt ttggcggcgg aggattacaa cctggagaat tatctggaaa
      541 agagttttct aaactcactt gtagtagtat ttttgaagcg tcaaaaaatg a
//
LOCUS       DARNVM010000369          585 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.371, whole genome shotgun sequence.
ACCESSION   DARNVM010000369 DARNVM010000000
VERSION     DARNVM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.371"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(107..262)
                     /locus_tag="SI621_004815"
     CDS             complement(107..262)
                     /locus_tag="SI621_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067409.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAMRLLN"
     gene            complement(342..407)
                     /locus_tag="SI621_004816"
     CDS             complement(342..407)
                     /locus_tag="SI621_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067410.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(410..>585)
                     /locus_tag="SI621_004817"
     CDS             complement(410..>585)
                     /locus_tag="SI621_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEI4067411.1"
                     /translation="HFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITFDNISQ
                     YLNASRLSLDLKK"
BASE COUNT          198 a          112 c          102 g          173 t
ORIGIN      
        1 tataaaagat aagatgaaac atcgcaataa tcaacatacg acggtctaaa tttcacacaa
       61 acagataaag atgattatca tttattatca atgcattaga atcaaatcaa ttcaagagtc
      121 tcattgctgc ttccagaatt tcttctgaag ttacatgtcg atccgctgct acataaatga
      181 ctttatgatc tccggtcaga gatggaaacc ctgcggccat tacagtaagg tgtgtttttt
      241 cgccatttgg atattcacgc atgatggtgt taactccagt catcgctggc attaccactg
      301 ctggttcaga gttaaaaaaa actatgattt ttttcatgat gttaccgtag tatgtgagta
      361 tccatcgaat agacaccaag caaaaaagct cccgaaggag ccttcatttt cactttttta
      421 aatccaacga cagacggctg gcatttaagt attgtgaaat attatcaaat gtaatcatca
      481 ttgatttaca aaagatacat tttgccccga aaggattcat gtcagaaaca tcaaaagatg
      541 atgttctata ctgggaacca tgacaacacg ggcatctaaa gtgaa
//
LOCUS       DARNVM010000370          573 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.372, whole genome shotgun sequence.
ACCESSION   DARNVM010000370 DARNVM010000000
VERSION     DARNVM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.372"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>573
                     /locus_tag="SI621_004818"
     CDS             <1..>573
                     /locus_tag="SI621_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEI4067412.1"
                     /translation="QKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHP
                     SIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQ
                     IKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLIK
                     AILLRLDMEQQDESVIKTYTGRITIEHILP"
BASE COUNT          194 a           85 c          114 g          180 t
ORIGIN      
        1 tacaaaaagg ttttgaagct tttattgaaa atctccagca gcaatttgat ggagatgcta
       61 ttgctatgtc actgatgttg gttaattctg caaaaaatta tactaagatt ttagagaatg
      121 attttgaaca cccttcaata cgacgaaaaa tagcatcgct aagtaattta ggggtagatg
      181 agtggattcc tccggttatg gcattcatga atcgaatggc tcgtacagaa gattttaatt
      241 tagatgattt ttctcaattt attaccgcct ttgagaaggt atatatgcat gggtggttaa
      301 aaaaacaaat taaaagccaa agagaaatgg tttgttattc agctctggta gcaataaata
      361 atgatatgcc ttttgattct gtgataaatc agataaacca acatgcagat aattcaggtt
      421 ttatagctgc tctggatgaa gatttatatg agcctcgtcc gaatcaagtg aacttaataa
      481 aagcaatttt gcttcgctta gacatggagc aacaagatga gagcgtgatt aaaacctata
      541 cggggagaat cacaattgag catattcttc ctc
//
LOCUS       DARNVM010000371          560 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.373, whole genome shotgun sequence.
ACCESSION   DARNVM010000371 DARNVM010000000
VERSION     DARNVM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.373"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>560
                     /locus_tag="SI621_004819"
     rRNA            <1..>560
                     /locus_tag="SI621_004819"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          141 a          126 c          183 g          110 t
ORIGIN      
        1 ggggaggaag ggagtaaagt taataccttt gctcattgac gttacccgca gaagaagcac
       61 cggctaactc cgtgccagca gccgcggtaa tacggagggt gcaagcgtta atcggaatta
      121 ctgggcgtaa agcgcacgca ggcggtttgt taagtcagat gtgaaatccc cgggctcaac
      181 ctgggaactg catctgatac tggcaagctt gagtctcgta gaggggggta gaattccagg
      241 tgtagcggtg aaatgcgtag agatctggag gaataccggt ggcgaaggcg gccccctgga
      301 cgaagactga cgctcaggtg cgaaagcgtg gggagcaaac aggattagat accctggtag
      361 tccacgccgt aaacgatgtc gacttggagg ttgtgccctt gaggcgtggc ttccggagct
      421 aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga
      481 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacctta
      541 cctggtcttg acatccacag
//
LOCUS       DARNVM010000372          550 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.374, whole genome shotgun sequence.
ACCESSION   DARNVM010000372 DARNVM010000000
VERSION     DARNVM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.374"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>550
                     /locus_tag="SI621_004820"
     CDS             <1..>550
                     /locus_tag="SI621_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEI4067413.1"
                     /translation="DTETLTVSYEFILCQHLIANTEFSYLALPENYNCLFLPNSKNQT
                     NNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIA
                     TASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIETIYIKRTSKDLANLRKEFN
                     KTVRKNFLIKLAKTSEASERFNA"
BASE COUNT          217 a           93 c           93 g          147 t
ORIGIN      
        1 tggatacaga aacattaact gttagttatg aatttatcct ctgtcaacat cttatcgcaa
       61 ataccgagtt cagctatttg gcactccctg aaaattataa ctgtttgttt ttaccaaatt
      121 caaaaaacca aacaaataat cgtttcaaaa cgttaaacag caaagctatt ggcaggctac
      181 ttgctgctgg tggcgtatac aatgggaaca ttgaaggatt cagagatact gcggaaaaac
      241 tgggtggaga tgcaataaaa ggctatgatc aaatactaaa tgaaaaaaca gcgggcatag
      301 cgatagcaac agcatctatt cttttaacaa agcgttctaa tgttgataca tatacagaaa
      361 taaatagtta cttaggcaaa cttagaggtc aacaaaaact tcttgatggt atagacataa
      421 tagaaacaat atacattaag agaacttcaa aagacttagc taacttacga aaggagttta
      481 ataaaactgt aagaaaaaat tttcttatca aacttgcaaa aacctccgaa gcatctgaaa
      541 gattcaacgc
//
LOCUS       DARNVM010000373          542 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.375, whole genome shotgun sequence.
ACCESSION   DARNVM010000373 DARNVM010000000
VERSION     DARNVM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.375"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..481)
                     /gene="fimE"
                     /locus_tag="SI621_004821"
     CDS             complement(<1..481)
                     /gene="fimE"
                     /locus_tag="SI621_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HEI4067414.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     "
BASE COUNT          153 a          143 c          116 g          130 t
ORIGIN      
        1 cgagataatc ctgaattaaa cgggtatcgg caccacgctc cgccagttca taaccgcaag
       61 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata cctgcatcgc
      121 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag
      181 cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt
      241 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
      301 ttattcgacc ttcattaagg tcaagatcct gataatgcag atcaagcagt tcactaatac
      361 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
      421 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
      481 caatagtagt actcctgact gagatatatt tgaatgaata cctataggaa acctcaatcg
      541 gt
//
LOCUS       DARNVM010000374          530 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.376, whole genome shotgun sequence.
ACCESSION   DARNVM010000374 DARNVM010000000
VERSION     DARNVM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.376"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>530
                     /gene="tuf"
                     /locus_tag="SI621_004822"
     CDS             <1..>530
                     /gene="tuf"
                     /locus_tag="SI621_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEI4067415.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGR"
BASE COUNT          128 a          131 c          148 g          123 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggcc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg
//
LOCUS       DARNVM010000375          529 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.377, whole genome shotgun sequence.
ACCESSION   DARNVM010000375 DARNVM010000000
VERSION     DARNVM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.377"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>529
                     /gene="eilA"
                     /locus_tag="SI621_004823"
     CDS             <1..>529
                     /gene="eilA"
                     /locus_tag="SI621_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HEI4067416.1"
                     /translation="IILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLH
                     ENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELV
                     QGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIEL
                     IHARTHKLIDHQSID"
BASE COUNT          152 a           92 c          105 g          180 t
ORIGIN      
        1 ttattattct attgcttaat gctgacggtg aaattgttag caaagaagag attatcgata
       61 aggtctggag cgcgagtgtt gccagtgatg aatctctaac acgatgtatt tatgcattgc
      121 gcaaactttt gcatgagaat aaacagtgta agtatatcga aacggtctat ggtcgtggat
      181 accgttttac tgtacctatt gtgattgtga ccgataatga accggtaaag tcgaccacta
      241 ctcttgccgt ttttcccttt cgtactgaag gttcgatcaa tgttctaaaa ctccattacg
      301 aacttgtcca gggcctttca aaatatgctt tttgtggttt agatatttta cccgcttcgg
      361 taacaaatga ggccagtgat ttttccacta tccatcagtt tatcaataaa acaggtccag
      421 aatattatat tatgggtcag gtcgttcact atggtaaaaa ctggcgttta tttattgagt
      481 taattcatgc cagaacacat aaattgattg atcatcaaag tattgattt
//
LOCUS       DARNVM010000376          527 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.378, whole genome shotgun sequence.
ACCESSION   DARNVM010000376 DARNVM010000000
VERSION     DARNVM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.378"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>527
                     /gene="wzx"
                     /locus_tag="SI621_004824"
     CDS             <1..>527
                     /gene="wzx"
                     /locus_tag="SI621_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O2/O50 family O-antigen flippase"
                     /protein_id="HEI4067417.1"
                     /translation="VLFCLNKSWFRFKYWWGKTDKDKIIKIIHYTLMALVSVISMPTA
                     LMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALSTYFLPRLTIIKTSFLIKKE
                     VNSTILYIVSITSFMALSIYLFRDLVITVLFTEQFRSARELFLLQLVGDVIKIAGFLY
                     AYPLQSQGHTKLFIS"
BASE COUNT          162 a           75 c           98 g          192 t
ORIGIN      
        1 tagttttatt ttgtctcaat aaatcttggt ttagatttaa atattggtgg ggtaaaacgg
       61 ataaagacaa aattataaaa attattcatt atactctgat ggctttggtt tctgttatct
      121 ccatgcctac agcattgatg tgtattagaa aaatattgat tgctaaaact ggttgggagg
      181 atgcagggca atggcaggcc gtatggaaga tatctgaggt ctatcttggt gttgtgacaa
      241 ttgctttgtc aacatatttc ttaccaagat tgacaattat aaaaacaagt ttccttataa
      301 aaaaagaggt aaatagtact atattataca tcgtatctat tacttcattc atggcgttga
      361 gtatctattt attccgcgat ttggtaataa cagttttatt tactgaacag tttcgctcag
      421 ctcgtgaatt atttttatta caactcgtag gggatgtaat aaaaattgct gggtttcttt
      481 atgcataccc tcttcaaagt caggggcata ctaaactatt catcagt
//
LOCUS       DARNVM010000377          472 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.379, whole genome shotgun sequence.
ACCESSION   DARNVM010000377 DARNVM010000000
VERSION     DARNVM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.379"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..323
                     /locus_tag="SI621_004825"
     CDS             <1..323
                     /locus_tag="SI621_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEI4067418.1"
                     /translation="DYQDNIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNG
                     DATPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKRP
                     ALGY"
BASE COUNT          177 a           86 c          103 g          106 t
ORIGIN      
        1 tggactatca agataatata gccctcgagc aagtcgtaac atgggattct gataatatat
       61 tcacacctaa tatctttgaa cataatggaa gatgttttat gaagaaaaat gaaacatgga
      121 tgttattaaa tggagatgca acaccaaaca ttagaacaat gagcaataag aaaattgaat
      181 gcctggagaa gagtgaagta gaatgggcta gtagggtttg cgacagtatc acagacaagg
      241 aaagggaaaa tctgaaggca ttaatatgta gcaaaccagc taaatcagtt aatacaggca
      301 aaagaccagc gttaggatat tgaaaccggc actgcactat attactatta caatattctg
      361 agtgactaga gcaactgctc cgccatccgg gctagcagta tgacgacgaa tccgaactgc
      421 actacaactg gcaccggtac tacaatccgg gacagggacg atatatcaca ca
//
LOCUS       DARNVM010000378          449 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.380, whole genome shotgun sequence.
ACCESSION   DARNVM010000378 DARNVM010000000
VERSION     DARNVM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.380"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..>449
                     /locus_tag="SI621_004826"
     CDS             <1..>449
                     /locus_tag="SI621_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase"
                     /protein_id="HEI4067419.1"
                     /translation="WSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHENIAKNQLAREKLIIYNSEGNYNIVDS
                     VDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIV"
BASE COUNT          141 a           82 c           85 g          141 t
ORIGIN      
        1 catggtcggt ggcttcagat gactctaaca acagctggaa tccggcctac aattgctttg
       61 atcgtccggg taaaaaatgt atctctttta cgctacgcat caacggcaaa gatcacgatt
      121 tgttagcggt ggataaaata catgtcgatt taaactggcg atatctgaac gagtatcttg
      181 atcaaatcag cgctaatgat gaagttctat ttttgaaaca aggccatgag aacattgcca
      241 agaatcaact cgcgcgtgaa aaactgatta tttataatag cgaaggtaat tataatattg
      301 ttgattctgt cgatactgaa tatatcgaaa aaacatcagc ggtgccaaac aacgcattat
      361 tcgaaatcta tttttattat cctggcggta atttattgaa cgcatcagat aaactttttt
      421 atctgccgtt tgctttcatt attatcgta
//
LOCUS       DARNVM010000379          440 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.381, whole genome shotgun sequence.
ACCESSION   DARNVM010000379 DARNVM010000000
VERSION     DARNVM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.381"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..>440)
                     /locus_tag="SI621_004827"
     CDS             complement(<1..>440)
                     /locus_tag="SI621_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEI4067420.1"
                     /translation="TDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGDCNFT
                     TATVDNVIFKCRRLHNVIFIKASGEYVDFSQSILDTVDFSRSQLTHSNFRECQIRNSK
                     FNNCYLYASHFTRAEFLSDKEISFIKSNLTAVVFDHVRISTGNF"
BASE COUNT          156 a           81 c           68 g          135 t
ORIGIN      
        1 ttaaaattcc ctgtcgatat tcgcacatga tcaaacacga cagctgtcaa attcgattta
       61 ataaatgata tttctttgtc agaaagaaat tctgctctgg tgaagtgcga agcataaaga
      121 taacaattat tgaactttga atttctaatc tgacattcac gaaaattact atgagtaagc
      181 tggctccgcg agaagtcaac tgtatcaaga atactttggc taaaatcgac atattcgcca
      241 ctcgctttga taaaaatcac attgtgtaaa cgtcgacatt taaatatgac attatcaact
      301 gttgcagtag taaaattgca atcccctatt tctttacatt cgaatgttac atgttgtaga
      361 tttgacgata aaaaagacac cttcgaaata acgtcacctt taaaatgaca acttgttaaa
      421 tccaaatcag acaggtcagt
//
LOCUS       DARNVM010000380          431 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.denovo.382, whole genome shotgun sequence.
ACCESSION   DARNVM010000380 DARNVM010000000
VERSION     DARNVM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.denovo.382"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
BASE COUNT          138 a           62 c           73 g          158 t
ORIGIN      
        1 taaagaaaag tattttcttc tgttagaatg ttttttattt gaattaatct gttccactat
       61 tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat tcattcttca
      121 tatcaaacac agtgcatgtg ttgcacattg tcccaggaac gttggaggcc taatttaaat
      181 gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa ttcttacgca
      241 tgtaggatta gtgagaagac ttatagtgct aatgtgaaac tataaatcat cggtacaatc
      301 cctgatttta ttgttgacat ttcatttatg ccgactattt atatggtata cttgtcgaat
      361 tatcttaaag gaagctctaa ttttcttatt tttattgaga aaatgagatg atgccttatg
      421 tctgtattac t
//
LOCUS       DARNVM010000381         3369 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.guided.269, whole genome shotgun sequence.
ACCESSION   DARNVM010000381 DARNVM010000000
VERSION     DARNVM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.guided.269"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            complement(<1..1148)
                     /gene="pepD"
                     /locus_tag="SI621_004828"
     CDS             complement(<1..1148)
                     /gene="pepD"
                     /locus_tag="SI621_004828"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HEI4067421.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLG"
     gene            1409..1867
                     /gene="gpt"
                     /locus_tag="SI621_004829"
     CDS             1409..1867
                     /gene="gpt"
                     /locus_tag="SI621_004829"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEI4067422.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1959..3203
                     /gene="frsA"
                     /locus_tag="SI621_004830"
     CDS             1959..3203
                     /gene="frsA"
                     /locus_tag="SI621_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HEI4067423.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3261..>3369
                     /locus_tag="SI621_004831"
     CDS             3261..>3369
                     /locus_tag="SI621_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crl family RNA polymerase assembly factor"
                     /protein_id="HEI4067424.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFD"
BASE COUNT          809 a          855 c          864 g          841 t
ORIGIN      
        1 cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag agaacggatc
       61 aggcagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt cagggaggtt
      121 tcgaccacac cttttgccac gtcggagttg cggatcacac cgttcggggt ggcgttcagc
      181 agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt cgcaacagag
      241 tccagcagca atgccagatt tttctctttc tctgccagct cgtttttcag gatttcctga
      301 taggtattca ccagagattt cagggcgtcg actttatcag ctgcgacagc aatggtcgca
      361 aaggcttcac gcgggatggc gttacgcagt gtgccaccgt tgaaatcgat aaggcgcagg
      421 tccagctctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc attacccagg
      481 ccaacgtgga tttcaccgcc ggagtgaccg ccttttagac cttttaaggt taacttgaag
      541 gtttcaaaac cagctggaac tgcttcacga tctaaatgca ggttggaggt gaagtcgata
      601 ccccccgcgc aacccatgta gatttcacct tcctcttcgg agtcggtgtt aatcagaata
      661 tcagcctgca accagttgct ctgtaagcca aaagcaccat ccataccggc ttcttcggtc
      721 atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag aaccgccagc
      781 gcagacgcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc tttaacccat
      841 tcgccatcaa tataaggctg gataggatct ttagtgaagt catgcacggt gtcgttattt
      901 ttctgtggca ccatatcgag gtgggcctgc aagacgaccg gtttacgatt ttccatacct
      961 gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct
     1021 ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga
     1081 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
     1141 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
     1201 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     1261 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     1321 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     1381 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     1441 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     1501 cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc gtgaactggg
     1561 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     1621 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     1681 ggataccggt ggcactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     1741 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     1801 agatacctgg atcgaacaac cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     1861 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     1921 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     1981 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     2041 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     2101 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgt gaaatcctcg
     2161 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     2221 cggtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag gcgatggtgt
     2281 ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgtcac tggctgcatg
     2341 cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac ctggccgagc
     2401 aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc tcagcgtctg ccgggcacca
     2461 tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt ttgcatatgc
     2521 cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat gcgatgcaga
     2581 cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg atgctgacta
     2641 ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac tccagcctgt
     2701 tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtagatcac actcgcgtcg
     2761 cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac cttgaatcgc
     2821 cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg agtgatttta
     2881 agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagccgt ttggggatgc
     2941 atgatgcttc cgacgaagcg ttgcgcgtgg agctgaatcg ctattcatta aaagtgcaag
     3001 gattgctggg acgtcgctgc ccaacgccaa tgttatcagg ctactggaag aacgatccgt
     3061 tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt aaattgttag
     3121 agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa ataacagact
     3181 ggattgaaaa acgattgtgt taaaaatttg ctaaattttg ccaatttggt aaaacagttg
     3241 catcacaaca ggagattgca atgacgttac cgagtggaca cccgaagagc agattgatca
     3301 aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat aatcgattct
     3361 ttttcgatt
//
LOCUS       DARNVM010000382         2357 bp    DNA     linear   BCT 16-NOV-2023
DEFINITION  TPA_asm: Escherichia coli strain AB_FA15_PTC_1.4
            SAMN32739125-rid17909513.guided.287, whole genome shotgun sequence.
ACCESSION   DARNVM010000382 DARNVM010000000
VERSION     DARNVM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32739125
            Sequence Read Archive: SRR23081329
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718138.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/03/2023 22:13:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,744
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 56 of 88
            Pseudo Genes (internal stop)      :: 25 of 88
            Pseudo Genes (multiple problems)  :: 24 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32739125-rid17909513.guided.287"
                     /strain="AB_FA15_PTC_1.4"
                     /isolation_source="hypereutrophic water [ENVO:01001018]"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="42.7822 N 86.00261 W"
                     /collection_date="2015-08-25"
     gene            <1..305
                     /gene="frdD"
                     /locus_tag="SI621_004832"
     CDS             <1..305
                     /gene="frdD"
                     /locus_tag="SI621_004832"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEI4067425.1"
                     /translation="AGGMWSAIIAPVMILLVGILLPLGLFPGDALSYERVLAFAQSFI
                     GRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            368..1501
                     /locus_tag="SI621_004833"
     CDS             368..1501
                     /locus_tag="SI621_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HEI4067426.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1589..2122
                     /gene="blc"
                     /locus_tag="SI621_004834"
     CDS             1589..2122
                     /gene="blc"
                     /locus_tag="SI621_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HEI4067427.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(2119..>2357)
                     /gene="sugE"
                     /locus_tag="SI621_004835"
     CDS             complement(2119..>2357)
                     /gene="sugE"
                     /locus_tag="SI621_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEI4067428.1"
                     /translation="RLTPSVITVTAMIVSMALLAWAMKSLPVGTAYAVWTGIGAVGAA
                     ITGIVLLGESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT          567 a          617 c          618 g          555 t
ORIGIN      
        1 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
       61 tgctgccgct ggggctgttt ccgggtgatg cactgagcta cgagcgcgtt ctggcgttcg
      121 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
      181 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct gcgggcaaat
      241 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa
      301 tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
      361 agaccctatg ttcaaaatga cgctctgcgc cttattaatt accgcctctt gctccacatt
      421 tgctgcccct caacaaatca acgatattgt gcatcgcaca ataaccccgc ttatagagca
      481 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
      541 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
      601 aggctcggtc agcaaaacat ttacgggcgt acttggtggc gacgctattg cacgagggga
      661 aatcaagtta agcgatcccg cgacaaaata ctggcctgaa cttaccgcta aacaatggaa
      721 tgggatcaca ctattacatc tcgcaaccta caccgctggc ggcctgccat tgcaggtgcc
      781 ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc aacctgcatg
      841 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
      901 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
      961 caaactcact catacgtgga ttaatgtgcc gtccgcagaa gaaaagaatt acgcctgggg
     1021 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     1081 tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa aaccccttga
     1141 tatcactgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     1201 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacat
     1261 catcattaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa aagcgattac
     1321 gcccccagct cctgcagtgc gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     1381 tggtagctat gttgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     1441 aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta
     1501 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     1561 cttagaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
     1621 agctgcattt ctggtggttg cctgtagttc tcccacacca ccgcgtggcg tgaccgtggt
     1681 aaataatttc gataccaaac gctatctggg tacatggtat gagattgccc gttttgatca
     1741 ccgttttgaa cgcgggctgg agaaagtgac cgcgacgtat agcctgcggg atgacggcgg
     1801 cctgaatgtc attaataaag gctataaccc ggacagagag atgtggcagc agagtgaagg
     1861 aaaagcctac tttactggag atccgcgtcg cgcagccctg aaagtgtcgt tctttgggcc
     1921 attctacggc ggttataatg tcatcgcgct cgaccgggag taccgccacg cgctggtttg
     1981 cggcccggac cgcgactacc tgtggatact ctctcgcacg ccaaccattt ctgacgaagt
     2041 gaaacaggag atgctggcaa tcgcgacccg ggaagggttt gatatcagta agtttatttg
     2101 ggtacagcaa cccggtagtt aatgagtgct gagtttcaga ccaataatcc ccaatacgat
     2161 taacgccaga ctcgccaggc gcatcgggtt agcggactcg ccgagcagca caatgccggt
     2221 gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa
     2281 cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgccgtca cagtaataac
     2341 acttggcgtc aaacgac
//
